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Sample records for activity iii project

  1. SELENIUM TREATMENT/REMOVAL ALTERNATIVES DEMONSTRATION PROJECT - MINE WASTE TECHNOLOGY PROGRAM ACTIVITY III, PROJECT 20

    EPA Science Inventory

    This document is the final report for EPA's Mine WAste Technology Program (MWTP) Activity III, Project 20--Selenium Treatment/Removal Alternatives Demonstration project. Selenium contamination originates from many sources including mining operations, mineral processing, abandoned...

  2. Project ACE Activity Sets. Book III: Grades 8 through 12.

    ERIC Educational Resources Information Center

    Eden City Schools, NC.

    Eleven activity sets for students in grades 8 through 12 are designed to supplement courses in citizenship and U.S. history and government. "The Civil War That Could Have Been" creates a hypothetical situation which requires the participant to analyze the causes of the Civil War. In "History on TV -- Enemy or Ally of the Social Studies Program,"…

  3. The OPTX Project. IV. How Reliable is [O III] as a Measure of AGN Activity?

    NASA Astrophysics Data System (ADS)

    Trouille, L.; Barger, A. J.

    2010-10-01

    We compare optical and hard X-ray identifications of active galactic nuclei (AGNs) using a uniformly selected (above a flux limit of f 2-8 keV = 3.5 × 10-15 erg cm-2 s-1) and highly optically spectroscopically complete (>80% for f 2-8 keV > 10-14 erg cm-2 s-1 and >60% below) 2-8 keV sample observed in three Chandra fields (CLANS, CLASXS, and the CDF-N). We find that empirical emission-line ratio diagnostic diagrams misidentify 50% of the X-ray-selected AGNs that can be put on these diagrams as star formers. We confirm that there is a large (two orders of magnitude) dispersion in the ratio of the [O III]λ5007 (hereafter [O III]) to hard X-ray luminosities for the non-broad-line AGNs, even after applying reddening corrections to the [O III] luminosities. We find that the dispersion is similar for the broad-line AGNs, where there is not expected to be much X-ray absorption from an obscuring torus around the AGN nor much obscuration from the galaxy along the line of sight if the AGN is aligned with the galaxy. We postulate that the X-ray-selected AGNs that are misidentified by the diagnostic diagrams have low [O III] luminosities due to the complexity of the structure of the narrow-line region, which causes many ionizing photons from the AGN not to be absorbed. This would mean that the [O III] luminosity can only be used to predict the X-ray luminosity to within a factor of ~3 (1σ). Despite selection effects, we show that the shapes and normalizations of the [O III] and transformed hard X-ray luminosity functions show reasonable agreement, suggesting that the [O III] samples are not finding substantially more AGNs at low redshifts than hard X-ray samples. Some of the data presented herein were obtained at the W. M. Keck Observatory, which is operated as a scientific partnership among the California Institute of Technology, the University of California, and the National Aeronautics and Space Administration. The observatory was made possible by the generous financial

  4. The Decovalex III Project: A Summary of Activities and LessonsLearned

    SciTech Connect

    Tsang, Chin-Fu; Jing, Lanru; Stephansson, Ove; Kautsky, Fritz

    2005-03-21

    Initiated in 1992, the DECOVALEX project is an international collaboration for advancing the understanding and modeling of coupled thermo-hydro-mechanical (THM) processes in geologic systems. The project has made important scientific achievements through three stages and is progressing in its fourth stage. It has played a key role in the development of mathematical modeling and in situ testing of coupled THM processes in fractured rock and buffer/backfill materials, a subject of importance for performance assessment of radioactive waste geologic repositories. This paper summarizes studies under the most recent stage of the project, DECOVALEX III (2000-2003). These studies include those of two major field experiments: (a) the FEBEX experiment at Grimsel, Switzerland, investigating coupled THM processes in a crystalline rock-bentonite system, and (b) the Drift Scale Test (DST) experiment at Yucca Mountain, Nevada, investigating coupled THM processes in unsaturated tuff. These are two of the largest multiyear heater tests undertaken to date for the study of coupled THM processes in geological systems. In addition, three so-called benchmark tests are also studied to evaluate the impact of coupled THM processes under different scenarios and geometries. Within the DECOVALEX project, multiple research teams participated in each of the studies, using different approaches and computer codes. Comparisons of results have provided insight into coupled THM processes, which in turn has stimulated further development of our modeling capabilities. Lessons learned from these studies are discussed. The scientific advances and enhanced insight gained through this kind of international cooperation illustrate the effectiveness of the DECOVALEX project.

  5. The Megamaser Cosmology Project. III. Accurate Masses of Seven Supermassive Black Holes in Active Galaxies with Circumnuclear Megamaser Disks

    NASA Astrophysics Data System (ADS)

    Kuo, C. Y.; Braatz, J. A.; Condon, J. J.; Impellizzeri, C. M. V.; Lo, K. Y.; Zaw, I.; Schenker, M.; Henkel, C.; Reid, M. J.; Greene, J. E.

    2011-01-01

    Observations of H2O masers from circumnuclear disks in active galaxies for the Megamaser Cosmology Project (MCP) allow accurate measurement of the mass of supermassive black holes (BH) in these galaxies. We present the Very Long Baseline Interferometry images and kinematics of water maser emission in six active galaxies: NGC 1194, NGC 2273, NGC 2960 (Mrk 1419), NGC 4388, NGC 6264 and NGC 6323. We use the Keplerian rotation curves of these six megamaser galaxies, plus a seventh previously published, to determine accurate enclosed masses within the central ~0.3 pc of these galaxies, smaller than the radius of the sphere of influence of the central mass in all cases. We also set lower limits to the central mass densities of between 0.12 × 1010 and 61 × 1010 M sun pc-3. For six of the seven disks, the high central densities rule out clusters of stars or stellar remnants as the central objects, and this result further supports our assumption that the enclosed mass can be attributed predominantly to a supermassive BH. The seven BHs have masses ranging between 0.75 × 107 and 6.5 × 107 M sun, with the mass errors dominated by the uncertainty of the Hubble constant. We compare the megamaser BH mass determination with BH mass measured from the virial estimation method. The virial estimation BH mass in four galaxies is consistent with the megamaser BH mass, but the virial mass uncertainty is much greater. Circumnuclear megamaser disks allow the best mass determination of the central BH mass in external galaxies and significantly improve the observational basis at the low-mass end of the M-σsstarf relation. The M-σsstarf relation may not be a single, low-scatter power law as originally proposed. MCP observations continue and we expect to obtain more maser BH masses in the future.

  6. The Iceland Deep Drilling Project: (III) Evidence for amphibolite grade contact metamorphism in an active geothermal system

    NASA Astrophysics Data System (ADS)

    Marks, N.; Schiffman, P.; Zierenberg, R. A.; Franzson, H.

    2008-12-01

    One of the scientific goals of the Iceland Deep Drilling Project is to reach the depths of transition from greenschist to amphibolite grade metamorphism in an active geothermal system. The deepest borehole to date in the Reykjanes system is RN-17, which was drilled to a depth of 3082 m. This well had been considered as a candidate for deepening by the IDDP until it collapsed during a flow test in November 2005. Temperatures in the lower portion of the borehole were never recorded due to an obstruction at 2100 m depth, but are estimated to be approximately 340°C. Epidote, albite, and actinolite are ubiquitous within pillow basalt, hyaloclastite, and in veins, implying that greenschist grade conditions have been attained throughout much of the well below approximately 1200 m. Intrusive lithologies constitute approximately 50% of the observed cuttings between 2600 and 2700 m. These intrusive rocks have produced small, but recognizable contact metamorphic effects characterized by granoblastic hornfels consisting of amphibolite grade assemblages of quartz + anorthite + diopside + magnetite + titanite. These have, in turn, been locally cut by actinolite veins, presumably reflective of the present-day, thermal state of the hydrothermal system at these depths. Based on their siliceous bulk composition, we believe the hornfels represent the thermally- recrystallized products of earlier-formed, hydrothermal veins consisting of quartz, epidote, and actinolite. The metamorphic plagioclase is distinctly more anorthitic (An90 to An98) than igneous plagioclase in adjacent mafic intrusives (An33 to An80) and also exhibits consistently lower Mg content and higher iron (up to 2.07 wt.% as Fe2O3). Stoichiometry implies that much of the iron in hydrothermal anorthite is Fe3+, which may imply recrystallization from precursor epidote under relatively oxidizing conditions. Diopside compositions (average Wo0.48En0.27Fs0.25) are consistently less calcic than hydrothermal clinopyroxenes

  7. THE MEGAMASER COSMOLOGY PROJECT. III. ACCURATE MASSES OF SEVEN SUPERMASSIVE BLACK HOLES IN ACTIVE GALAXIES WITH CIRCUMNUCLEAR MEGAMASER DISKS

    SciTech Connect

    Kuo, C. Y.; Braatz, J. A.; Condon, J. J.; Impellizzeri, C. M. V.; Lo, K. Y.; Zaw, I.; Schenker, M.; Henkel, C.; Reid, M. J.; Greene, J. E.

    2011-01-20

    Observations of H{sub 2}O masers from circumnuclear disks in active galaxies for the Megamaser Cosmology Project (MCP) allow accurate measurement of the mass of supermassive black holes (BH) in these galaxies. We present the Very Long Baseline Interferometry images and kinematics of water maser emission in six active galaxies: NGC 1194, NGC 2273, NGC 2960 (Mrk 1419), NGC 4388, NGC 6264 and NGC 6323. We use the Keplerian rotation curves of these six megamaser galaxies, plus a seventh previously published, to determine accurate enclosed masses within the central {approx}0.3 pc of these galaxies, smaller than the radius of the sphere of influence of the central mass in all cases. We also set lower limits to the central mass densities of between 0.12 x 10{sup 10} and 61 x 10{sup 10} M{sub sun} pc{sup -3}. For six of the seven disks, the high central densities rule out clusters of stars or stellar remnants as the central objects, and this result further supports our assumption that the enclosed mass can be attributed predominantly to a supermassive BH. The seven BHs have masses ranging between 0.75 x 10{sup 7} and 6.5 x 10{sup 7} M{sub sun}, with the mass errors dominated by the uncertainty of the Hubble constant. We compare the megamaser BH mass determination with BH mass measured from the virial estimation method. The virial estimation BH mass in four galaxies is consistent with the megamaser BH mass, but the virial mass uncertainty is much greater. Circumnuclear megamaser disks allow the best mass determination of the central BH mass in external galaxies and significantly improve the observational basis at the low-mass end of the M-{sigma}{sub *} relation. The M-{sigma}{sub *} relation may not be a single, low-scatter power law as originally proposed. MCP observations continue and we expect to obtain more maser BH masses in the future.

  8. Innovative and Exemplary Projects in Missouri Schools [Title III, ESEA].

    ERIC Educational Resources Information Center

    Missouri State Dept. of Elementary and Secondary Education, Jefferson City.

    Forty-eight projects funded by the Elementary and Secondary Education Act Title III, and providing the funds to public school districts to demonstrate the feasibility of educational innovations, are described in this document about Missouri ESEA Title III exemplary programs. Nineteen projects completing the third year of operation, thirteen…

  9. A Systems Approach to the Development of an Evaluation System for ESEA Title III Projects.

    ERIC Educational Resources Information Center

    Yost, Marlen; Monnin, Frank J.

    A major activity of any ESEA Title III project is evaluation. This paper suggests evaluation methods especially appropriate to such projects by applying a systems approach to the evaluation design. Evaluation as a system is divided into three subsystems: (1) baseline evaluation, which describes conditions as they exist before project treatment;…

  10. Kenston Aerospace: Title III ESEA Project.

    ERIC Educational Resources Information Center

    Kenston Local School District, Chagrin Falls, OH.

    The objectives of a three-year comprehensive aerospace education program at Kenston High School, Chagrin Falls, Ohio, funded under Title III ESEA, were to provide marketable skills for non-College-bound students as well as counseling for the student planning on college or technical school education in the aviation field. Students also were taught…

  11. Space Processing Applications Rocket project SPAR III

    NASA Technical Reports Server (NTRS)

    Reeves, F.

    1978-01-01

    This document presented the engineering report and science payload III test report and summarized the experiment objectives, design/operational concepts, and final results of each of five scientific experiments conducted during the third Space Processing Applications Rocket (SPAR) flight flown by NASA in December 1976. The five individual SPAR experiments, covering a wide and varied range of scientific materials processing objectives, were entitled: Liquid Mixing, Interaction of Bubbles with Solidification Interfaces, Epitaxial Growth of Single Crystal Film, Containerless Processing of Beryllium, and Contact and Coalescence of Viscous Bodies.

  12. Breckinridge Project, initial effort. Report III, Volume 2. Specifications

    SciTech Connect

    1982-01-01

    Report III, Volume 2 contains those specifications numbered K through Y, as follows: Specifications for Compressors (K); Specifications for Piping (L); Specifications for Structures (M); Specifications for Insulation (N); Specifications for Electrical (P); Specifications for Concrete (Q); Specifications for Civil (S); Specifications for Welding (W); Specifications for Painting (X); and Specifications for Special (Y). The standard specifications of Bechtel Petroleum Incorporated have been amended as necessary to reflect the specific requirements of the Breckinridge Project and the more stringent specifications of Ashland Synthetic Fuels, Inc. These standard specifications are available for the Initial Effort (Phase Zero) work performed by all contractors and subcontractors.

  13. Modulation of active Cr(III) complexes by bath preparation to adjust Cr(III) electrodeposition

    NASA Astrophysics Data System (ADS)

    Li, Lei; Wang, Zhi; Wang, Ming-yong; Zhang, Yi

    2013-09-01

    The preparation process of the Cr(III) bath was studied based on a perspective of accelerating the formation of active Cr(III) complexes. The results of ultraviolet-visible absorption spectroscopy (UV-Vis) and electrodeposition showed that active Cr(III) complexes in the bath prepared at room temperature in several days were rare for depositing chromium. The increase of heating temperature, time, and pH value during the bath preparation promoted the formation of active Cr(III) complexes. The chromium deposition rate increased with the concentration of active Cr(III) complexes increasing. Increasing the heating temperature from 60 to 96°C, the chromium deposition rate increased from 0.40 to 0.71 μm/min. When the concentration of active Cr(III) complexes increased, the grain size of Cr coatings increased, and the carbon content of the coating decreased. It is deduced that Cr(H2O)4(OH)L2+ (L is an organic ligand, and its valence is omitted) is a primary active Cr(III) complex.

  14. Experimental and Theoretical Studies on Biologically Active Lanthanide (III) Complexes

    NASA Astrophysics Data System (ADS)

    Kostova, I.; Trendafilova, N.; Georgieva, I.; Rastogi, V. K.; Kiefer, W.

    2008-11-01

    The complexation ability and the binding mode of the ligand coumarin-3-carboxylic acid (HCCA) to La(III), Ce(III), Nd(III), Sm(III), Gd(III) and Dy(III) lanthanide ions (Ln(III)) are elucidated at experimental and theoretical level. The complexes were characterized using elemental analysis, DTA and TGA data as well as 1H NMR and 13C NMR spectra. FTIR and Raman spectroscopic techniques as well as DFT quantum chemical calculations were used for characterization of the binding mode and the structures of lanthanide(III) complexes of HCCA. The metal—ligand binding mode is predicted through molecular modeling and energy estimation of different Ln—CCA structures using B3LYP/6-31G(d) method combined with a large quasi-relativistic effective core potential for lanthanide ion. The energies obtained predict bidentate coordination of CCA- to Ln(III) ions through the carbonylic oxygen and the carboxylic oxygen. Detailed vibrational analysis of HCCA, CCA- and Ln(III) complexes based on both calculated and experimental frequencies confirms the suggested metal—ligand binding mode. The natural bonding analysis predicts strongly ionic character of the Ln(III)-CCA bonding in the- complexes studied. With the relatively resistant tumor cell line K-562 we obtained very interesting in-vitro results which are in accordance with our previously published data concerning the activity of lanthanide(III) complexes with other coumarin derivatives.

  15. [Plasma antithrombin III activity in patients with pulmonary thromboembolism].

    PubMed

    Vertun, B; Filipecki, S; Szczepański, M; Wawrzyńska, L; Rózycka, J

    A decreased plasma antithrombin III activity has been noted in 12 out of 20 patients. In 2 patients it was most probably congenital defect, whereas in the remaining 10 patients--acquired. The observed disorders in the activity of antithrombin III with particular reference to anticoagulant therapy have been discussed.

  16. 76 FR 81927 - Shiloh III Wind Project, LLC; Notice of Petition for Declaratory Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-29

    ... Energy Regulatory Commission Shiloh III Wind Project, LLC; Notice of Petition for Declaratory Order Take... III Wind Project, LLC filed a Petition for Declaratory Order, requesting that the Federal Energy... transaction for wind powered generation and related interconnection facilities located in Solano...

  17. Protein-water dynamics in antifreeze protein III activity

    NASA Astrophysics Data System (ADS)

    Xu, Yao; Bäumer, Alexander; Meister, Konrad; Bischak, Connor G.; DeVries, Arthur L.; Leitner, David M.; Havenith, Martina

    2016-03-01

    We combine Terahertz absorption spectroscopy (THz) and molecular dynamics (MD) simulations to investigate the underlying molecular mechanism for the antifreeze activity of one class of antifreeze protein, antifreeze protein type III (AFP-III) with a focus on the collective water hydrogen bond dynamics near the protein. After summarizing our previous work on AFPs, we present a new investigation of the effects of cosolutes on protein antifreeze activity by adding sodium citrate to the protein solution of AFP-III. Our results reveal that for AFP-III, unlike some other AFPs, the addition of the osmolyte sodium citrate does not affect the hydrogen bond dynamics at the protein surface significantly, as indicated by concentration dependent THz measurements. The present data, in combination with our previous THz measurements and molecular simulations, confirm that while long-range solvent perturbation is a necessary condition for the antifreeze activity of AFP-III, the local binding affinity determines the size of the hysteresis.

  18. Mostly Plants. Individualized Biology Activities on: I. Investigating Bread Mold; II. Transpiration; III. Botany Project; IV. Collecting/Preserving/Identifying Leaves; [and] V. Student Science Laboratory Write-Ups.

    ERIC Educational Resources Information Center

    Gibson, Paul R.

    Individualized biology activities for secondary students are presented in this teaching guide. The guide is divided into five sections: (1) investigating bread mold; (2) investigating transpiration; (3) completing a botany project; (4) collecting, preserving, and identifying leaves; and (5) writing up science laboratory investigations. The…

  19. National Project III, Elevating the Importance of Teaching. Fund Associate's Final Report.

    ERIC Educational Resources Information Center

    Seibert, Warren F.

    Purdue University's participation as a fund associate in National Project III (NP III) for elevating the importance of teaching has its origins in a flexible and diagnostic instructional evaluation system called "CAFETERIA." CAFETERIA services include test development, scoring, and analysis; social surveys on topics of importance in…

  20. Diverse intracellular pathogens activate type III interferon expression from peroxisomes.

    PubMed

    Odendall, Charlotte; Dixit, Evelyn; Stavru, Fabrizia; Bierne, Helene; Franz, Kate M; Durbin, Ann Fiegen; Boulant, Steeve; Gehrke, Lee; Cossart, Pascale; Kagan, Jonathan C

    2014-08-01

    Type I interferon responses are considered the primary means by which viral infections are controlled in mammals. Despite this view, several pathogens activate antiviral responses in the absence of type I interferons. The mechanisms controlling type I interferon-independent responses are undefined. We found that RIG-I like receptors (RLRs) induce type III interferon expression in a variety of human cell types, and identified factors that differentially regulate expression of type I and type III interferons. We identified peroxisomes as a primary site of initiation of type III interferon expression, and revealed that the process of intestinal epithelial cell differentiation upregulates peroxisome biogenesis and promotes robust type III interferon responses in human cells. These findings highlight the importance of different intracellular organelles in specific innate immune responses.

  1. ESCRT-III on endosomes: new functions, new activation pathway.

    PubMed

    Woodman, Philip

    2016-01-15

    The multivesicular body (MVB) pathway sorts ubiquitinated membrane cargo to intraluminal vesicles (ILVs) within the endosome, en route to the lysosomal lumen. The pathway involves the sequential action of conserved protein complexes [endosomal sorting complexes required for transport (ESCRTs)], culminating in the activation by ESCRT-II of ESCRT-III, a membrane-sculpting complex. Although this linear pathway of ESCRT activation is widely accepted, a study by Luzio and colleagues in a recent issue of the Biochemical Journal suggests that there is greater complexity in ESCRT-III activation, at least for some MVB cargoes. They show that ubiquitin-dependent sorting of major histocompatibility complex (MHC) class I to the MVB requires the central ESCRT-III complex but does not involve either ESCRT-II or functional links between ESCRT-II and ESCRT-III. Instead, they propose that MHC class I utilizes histidine-domain protein tyrosine phosphatase (HD-PTP), a non-canonical ESCRT interactor, to promote ESCRT-III activation.

  2. Project HEED, Title III, Section 306. Final Evaluation Report.

    ERIC Educational Resources Information Center

    Hughes, Orval D.

    Project HEED (Heed Ethnic Educational Depolarization) involves over 1,000 Indian children in grades 1-8 in Arizona. The project target sites are 48 classrooms at Sells, Topowa, San Carlos, Many Farms, Hotevilla, Peach Springs, and Sacaton. Objectives are to increase: (1) reading achievement, (2) affective behavior of teachers, (3) motivation by…

  3. An Evaluation of ESEA Title III Projects, Fiscal Year 1972. Interim Report.

    ERIC Educational Resources Information Center

    Wilson, Jerusa; And Others

    This report contains descriptions and progress of five projects in the District of Columbia partially or wholly funded by ESEA Title III: (1) The Columbia Road Preschool Pilot Project, a second-year experimental effort designed to serve as a model school providing an experimental setting for early childhood educational programs; (2) The Montessori…

  4. Special Education Music and Dance: An ESEA Title III Project Evaluation.

    ERIC Educational Resources Information Center

    Johnson, Dorothy H.; And Others

    Reported are the evaluation results on the 1969-70 segment (the first project period) of the Special Education Music and Dance Program in Shoreline School District 412 (Seattle, Washington), an ESEA Title III project. The program, which is presented as a pilot attempt to develop functional program objectives and evaluation tools, provides music…

  5. 77 FR 2968 - Pomperaug Hydro Project, Andrew Peklo III; Notice Establishing Deadline for Comments and Reply...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Pomperaug Hydro Project, Andrew Peklo III; Notice Establishing Deadline for Comments and Reply Comments On December 15, 2011, the Commission issued notice that Office of Energy Projects staff will hold a site visit...

  6. Scaled Vitrification System III (SVS III) Process Development and Laboratory Tests at the West Valley Demonstration Project

    SciTech Connect

    V. Jain; S. M. Barnes; B. G. Bindi; R. A. Palmer

    2000-04-30

    At the West Valley Demonstration Project (WVDP),the Vitrification Facility (VF)is designed to convert the high-level radioactive waste (HLW)stored on the site to a stable glass for disposal at a Department of Energy (DOE)-specified federal repository. The Scaled Vitrification System III (SVS-III)verification tests were conducted between February 1995 and August 1995 as a supplemental means to support the vitrification process flowsheet, but at only one seventh the scale.During these tests,the process flowsheet was refined and optimized. The SVS-III test series was conducted with a focus on confirming the applicability of the Redox Forecasting Model, which was based on the Index of Feed Oxidation (IFO)developed during the Functional and Checkout Testing of Systems (FACTS)and SVS-I tests. Additional goals were to investigate the prototypical feed preparation cycle and test the new target glass composition. Included in this report are the basis and current designs of the major components of the Scale Vitrification System and the results of the SVS-III tests.The major subsystems described are the feed preparation and delivery, melter, and off-gas treatment systems. In addition,the correlation between the melter's operation and its various parameters;which included feed rate,cold cap coverage,oxygen reduction (redox)state of the glass,melter power,plenum temperature,and airlift analysis;were developed.

  7. 75 FR 38645 - Standards Improvement Project-Phase III

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-02

    ...The Occupational Safety and Health Administration (OSHA) is continuing its efforts to remove or revise outdated, duplicative, unnecessary, and inconsistent requirements in its safety and health standards. This effort builds on the success of Standards Improvement Project (SIP)--Phase I published on June 18, 1998, and SIP--Phase II published on January 5, 2005. The Agency believes that the......

  8. DPI Equity Leadership Project--Phase III. Final Report.

    ERIC Educational Resources Information Center

    Riley, Linda L.

    During its third phase in 1993-94, the Wisconsin Department of Public Instruction (DPI) Gender Equity Leadership Project provided technical assistance and staff development experiences to the Wisconsin Vocational Equity Leadership Cadre (WVELC). The technical assistance/staff development component prepared the cadre to assist local districts in…

  9. Klondike III/Biglow Canyon Wind Integration Project; Record of Decision, October 25, 2006.

    SciTech Connect

    United States. Bonneville Power Administration

    2006-10-25

    The Bonneville Power Administration (BPA) has decided to implement the Proposed Action identified in the Klondike III/Biglow Canyon Wind Integration Project Final Environmental Impact Statement (FEIS) (DOE/EIS-0374, September 2006). Under the Proposed Action, BPA will offer PPM Energy, Inc. (PPM) contract terms for interconnection of the proposed Klondike III Wind Project, located in Sherman County, Oregon, with the Federal Columbia River Transmission System (FCRTS). BPA will also offer Portland General Electric (PGE)1 contract terms for interconnection of its proposed Biglow Canyon Wind Farm, also located in Sherman County, Oregon, with the FCRTS, as proposed in the FEIS. To interconnect these wind projects, BPA will build and operate a 12-mile long, 230-kilovolt (kV) double-circuit transmission line between the wind projects and BPA's new 230-kV John Day Substation in Sherman County, Oregon. BPA will also expand its existing 500-kV John Day Substation.

  10. Cosmetology; Glossary of Key Words. Vocational Reading Power Project, Title III, E.S.E.A.

    ERIC Educational Resources Information Center

    Premer, LaVerne

    The glossary is one of twenty in various subject areas of vocational education designed to assist the student in vocabulary mastery for particular vocational education courses. They are part of the Vocational Reading Power Project, Title III, E.S.E.A. This glossary is for a course in cosmetology. It is divided into two parts: one provides the…

  11. Evaluation Reports of ESEA, Title III Projects: Final Report, FY 1972.

    ERIC Educational Resources Information Center

    District of Columbia Public Schools, Washington, DC. Dept. of Research and Evaluation.

    Through Title III of the Elementary Secondary Education Act of 1965, six programs were funded in Washington, D.C. public schools to meet the educational needs of handicapped disadvantaged children. One of the programs, the Columbia Road Pre-School Pilot Project was designed to serve as an experimental model for early childhood education for…

  12. A Formal Proposal for an ESEA Title III Project for Emotionally and Learning Disabled Students.

    ERIC Educational Resources Information Center

    Yankton Independent School District 1, SD.

    Presented is a proposal for a 2-year project to identify and remediate needs of emotionally and learning disabled (LD) children in kindergarten through grade 3 in Yankton, South Dakota, to be funded through the Elementary and Secondary Education Act, Title III. Defined in the preface are areas of learning disabilities and associated skills to be…

  13. Eisenhower Pre-Service Teacher Education Project, Higher Education Consortium Region III. Final Report.

    ERIC Educational Resources Information Center

    Wozniak, Jacci

    The Eisenhower Pre-Service Teacher Education Project was developed by the University of Central Florida, the five community colleges in Region III of the Higher Education Consortium, and the private college and universities in the same region to design curriculum changes to improve the preparation of elementary and secondary math and science…

  14. Oregon Small Schools Program: A Title III Project. Independent Evaluation Report.

    ERIC Educational Resources Information Center

    Educational Coordinates Northwest, Salem, OR.

    An independent evaluation report (May 1971) by Educational Coordinates Northwest, this document examines the Oregon Small Schools Program (a Title III project of the Elementary and Secondary Education Act). Three major areas were considered: the extent to which member schools implemented methodological and organizational changes, the established…

  15. Application of the BISON Fuel Performance Code to the FUMEX-III Coordinated Research Project

    SciTech Connect

    R. L. Williamson; S. R. Novascone

    2012-04-01

    INL recently participated in FUMEX-III, an International Atomic Energy Agency sponsored fuel modeling Coordinated Research Project. A main purpose of FUMEX-III is to compare code predictions to reliable experimental data. During the same time period, the INL initiated development of a new multidimensional (2D and 3D) multiphysics nuclear fuel performance code called BISON. Interactions with international fuel modeling researchers via FUMEX-III played a significant and important role in the BISON evolution, particularly influencing the selection of material and behavioral models which are now included in the code. BISON's ability to model integral fuel rod behavior did not mature until 2011, thus the only FUMEX-III case considered was the Riso3-GE7 experiment, which includes measurements of rod outer diameter following pellet clad mechanical interaction (PCMI) resulting from a power ramp late in fuel life. BISON comparisons to the Riso3-GE7 final rod diameter measurements are quite reasonable. The INL is very interested in participation in the next Fuel Modeling Coordinated Research Project and would like to see the project initiated as soon as possible.

  16. Monolithic active pixel matrix with binary counters (MAMBO III) ASIC

    SciTech Connect

    Khalid, Farah; Deptuch, Grzegorz; Shenai, Alpana; Yarema, Raymond; /Fermilab

    2010-01-01

    Monolithic Active Matrix with Binary Counters (MAMBO) is a counting ASIC designed for detecting and measuring low energy X-rays from 6-12keV. Each pixel contains analogue functionality implemented with a charge preamplifier, CR-RC{sup 2} shaper and a baseline restorer. It also contains a window comparator which can be trimmed by 4 bit DACs to remove systematic offsets. The hits are registered by a 12 bit ripple counter which is reconfigured as a shift register to serially output the data from the entire ASIC. Each pixel can be tested individually. Two diverse approaches have been used to prevent coupling between the detector and electronics in MAMBO III and MAMBO IV. MAMBO III is a 3D ASIC, the bottom ASIC consists of diodes which are connected to the top ASIC using {mu}-bump bonds. The detector is decoupled from the electronics by physically separating them on two tiers and using several metal layers as a shield. MAMBO IV is a monolithic structure which uses a nested well approach to isolate the detector from the electronics. The ASICs are being fabricated using the SOI 0.2 {micro}m OKI process, MAMBO III is 3D bonded at T-Micro and MAMBO IV nested well structure was developed in collaboration between OKI and Fermilab.

  17. Klondike III/Biglow Canyon Wind Integration Project; Final Environmental Impact Statement, September 2006.

    SciTech Connect

    United States. Bonneville Power Administration

    2006-09-01

    BPA has been asked by PPM Energy, Inc. to interconnect 300 megawatts (MW) of electricity generated from the proposed Klondike III Wind Project to the Federal Columbia River Transmission System. Orion Energy LLC has also asked BPA to interconnect 400 MW of electricity from its proposed Biglow Canyon Wind Farm, located north and east of the proposed Klondike III Wind Project. (Portland General Electric recently bought the rights to develop the proposed Biglow Canyon Wind Farm from Orion Energy, LLC.) Both wind projects received Site Certificates from the Oregon Energy Facility Siting Council on June 30, 2006. To interconnect these projects, BPA would need to build and operate a 230-kV double-circuit transmission line about 12 miles long, expand one substation and build one new substation. The wind projects would require wind turbines, substation(s), access roads, and other facilities. Two routes for the transmission line are being considered. Both begin at PPM's Klondike Schoolhouse Substation then travel north (Proposed Action) or north and westerly (Middle Alternative) to a new BPA 230-kV substation next to BPA's existing John Day 500-kV Substation. BPA is also considering a No Action Alternative in which BPA would not build the transmission line and would not interconnect the wind projects. The proposed BPA and wind projects would be located on private land, mainly used for agriculture. If BPA decides to interconnect the wind projects, construction of the BPA transmission line and substation(s) could commence as early as the winter of 2006-07. Both wind projects would operate for much of each year for at least 20 years. The proposed projects would generally create no or low impacts. Wildlife resources and local visual resources are the only resources to receive an impact rating other than ''none'' or ''low''. The low to moderate impacts to wildlife are from the expected bird and bat mortality and the cumulative impact of this project on wildlife when combined with

  18. 77 FR 13316 - FFP Project 91, LLC, Riverbank Hydro No. 23, LLC, Lock+ Hydro Friends Fund III; Notice Announcing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-06

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission FFP Project 91, LLC, Riverbank Hydro No. 23, LLC, Lock+ Hydro Friends Fund.... 2. Riverbank Hydro No. 23, LLC: Project No. 14279-000. 3. Lock+ Hydro Friends Fund III: Project...

  19. NOVEL CONCEPTS RESEARCH IN GEOLOGIC STORAGE OF CO2 PHASE III THE OHIO RIVER VALLEY CO2 STORAGE PROJECT

    SciTech Connect

    Neeraj Gupta

    2005-05-26

    As part of the Department of Energy's (DOE) initiation on developing new technologies for storage of carbon dioxide in geologic reservoir, Battelle has been awarded a project to investigate the feasibility of CO{sub 2} sequestration in the deep saline reservoirs in the Ohio River Valley region. This project is the Phase III of Battelle's work under the Novel Concepts in Greenhouse Gas Management grant. The main objective of the project is to demonstrate that CO{sub 2} sequestration in deep formations is feasible from engineering and economic perspectives, as well as being an inherently safe practice and one that will be acceptable to the public. In addition, the project is designed to evaluate the geology of deep formations in the Ohio River Valley region in general and in the vicinity of AEP's Mountaineer Power Plant in particular, in order to determine their potential use for conducting a long-term test of CO{sub 2} disposal in deep saline formations and potentially in nearby deep coal seams. The current technical progress report summarizes activities completed for the January through March 2005 period of the project. As discussed in the report, the technical activities focused on development of injection well design, preparing a Class V Underground Injection Control permit, assessment of monitoring technologies, analysis of coal samples for testing the capture system by Mitsubishi Heavy Industry, and presentation of project progress at several venues. In addition, related work has progressed on a collaborative risk assessment project with Japan research institute CREIPI and technical application for the Midwest Regional Carbon Sequestration Partnership.

  20. 15 CFR Appendix B to Subpart R of... - Minor Projects for Purposes of § 922.193(a)(2)(iii)

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ....193(a)(2)(iii) B Appendix B to Subpart R of Part 922 Commerce and Foreign Trade Regulations Relating... Thunder Bay National Marine Sanctuary and Underwater Preserve Pt. 922, Subpt. R, App. B Appendix B to Subpart R of Part 922—Minor Projects for Purposes of § 922.193(a)(2)(iii) Pursuant to Michigan...

  1. The Hmong Resettlement Study. Volume III: Exemplary Projects and Projects with Unique Features of Programmatic Interest.

    ERIC Educational Resources Information Center

    Minnesota Univ., Minneapolis. Center for Urban and Regional Affairs.

    This document, part of a national study of the resettlement experience of the Hmong in the United States, describes a variety of exemplary resettlement projects and strategies. They cover six major aspects of resettlement: (1) employment; (2) education and training; (3) income supplementation and economic development; (4) housing; (5) provision of…

  2. Project GIST (45-70-026). ESEA, Title III, Project Termination Report.

    ERIC Educational Resources Information Center

    Belpre City Schools, OH.

    This study evaluated a 2-year in-service teacher training project in the Belpre Middle School, Belpre, Ohio. The objectives of the program included a) the change of teachers' roles, in terms of perceptions, procedures, and morale, from those in self-contained classrooms to those in cooperative teaching teams; b) the increase of teachers'…

  3. 78 FR 41785 - Agency Information Collection Activities; Comment Request; Implementation of Title I/II Program...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-11

    ... Agency Information Collection Activities; Comment Request; Implementation of Title I/II Program... notice will be considered public records. Title of Collection: Implementation of Title I/II Program... Estimated Number of Annual Burden Hours: 6,573. Abstract: The Implementation of Title I/II...

  4. Synthesis and spectroscopic studies of biologically active compounds derived from oxalyldihydrazide and benzil, and their Cr(III), Fe(III) and Mn(III) complexes.

    PubMed

    Singh, D P; Kumar, Ramesh; Singh, Jitender

    2009-04-01

    A new series of complexes have been synthesized by template condensation of oxalyldihydrazide and benzil in methanolic medium in the presence of trivalent chromium, manganese and iron salts forming complexes of the type [M(C(32)H(24)N(8)O(4))X]X(2) where M = Cr(III), Mn(III), Fe(III) and X = Cl(-1), NO(3)(-1), CH(3)COO(-1). The complexes have been characterized with the help of elemental analyses, conductance measurements, magnetic susceptibility measurements, electronic, NMR, infrared and far infrared spectral studies. On the basis of these studies, a five coordinate square pyramidal geometry has been proposed for all these complexes. The biological activities of the metal complexes have been tested in vitro against a number of pathogenic bacteria to assess their inhibiting potential. Some of these complexes have been found to exhibit remarkable antibacterial activities.

  5. Results of a Survey Software Development Project Management in the U.S. Aerospace Industry. Volume III. Major Problems.

    DTIC Science & Technology

    1979-12-18

    DEVELOPMENT PROJECT MANAGEMENT IN THE U.S. AEROSPACE INDUSTRY VOLUME III MAJOR PROBLEMS ZZ: RICHARD H. THAYER SACRAMENTO AIR LOGISTICS CENTER tAIR FORCE...by block numbr) Software Engineering Project Management, Software Development , Survey, Project Management, Major Issues 20. AESTRACT (Continue oun... development projects. The sample of the U.S. Aerospace Industry that was surveyed consisted of companies with membership in the ALAA Technical

  6. Innovative Project Activities in Science [From the NSTA Study of Innovative Project Activities

    ERIC Educational Resources Information Center

    Science Teacher, 1975

    1975-01-01

    Describes four projects chosen as innovative project activities in science which exhibited identification of unique or novel problems and creative approaches to their solutions. Projects included a study of fish in Lake Erie, a goat raising project, an analysis of terrestrial plant ecology and soil composition, and a study of marine and wetlands…

  7. Calculus: An Active Approach with Projects.

    ERIC Educational Resources Information Center

    Hilbert, Stephen; And Others

    1993-01-01

    Discusses a pedagogical approach to calculus based on the question: What kinds of problems should students be able to solve? Includes a discussion of types of problems and curriculum threads for such a course. Describes a projects-based calculus with examples of projects and classroom activities. (Author/MDH)

  8. Project WET: Curriculum & Activity Guide. K-12.

    ERIC Educational Resources Information Center

    Nelson, Dennis; And Others

    Project WET (Water Education for Teachers) is a nonprofit water education program for educators and young people. This curriculum and activity guide for kindergarten through twelfth grades is a collection of innovative, water-related activities that are hands-on, easy to use, and fun. The activities incorporate a variety of formats such as large…

  9. Functional Activation of the Flagellar Type III Secretion Export Apparatus

    PubMed Central

    Phillips, Andrew M.; Calvo, Rebecca A.; Kearns, Daniel B.

    2015-01-01

    Flagella are assembled sequentially from the inside-out with morphogenetic checkpoints that enforce the temporal order of subunit addition. Here we show that flagellar basal bodies fail to proceed to hook assembly at high frequency in the absence of the monotopic protein SwrB of Bacillus subtilis. Genetic suppressor analysis indicates that SwrB activates the flagellar type III secretion export apparatus by the membrane protein FliP. Furthermore, mutants defective in the flagellar C-ring phenocopy the absence of SwrB for reduced hook frequency and C-ring defects may be bypassed either by SwrB overexpression or by a gain-of-function allele in the polymerization domain of FliG. We conclude that SwrB enhances the probability that the flagellar basal body adopts a conformation proficient for secretion to ensure that rod and hook subunits are not secreted in the absence of a suitable platform on which to polymerize. PMID:26244495

  10. Tunable luminescence and enhanced photocatalytic activity for Eu(III) doped Bi2WO6 nanoparticles.

    PubMed

    Gu, Haidong; Yu, Lei; Wang, Juan; Ni, Min; Liu, Tingting; Chen, Feng

    2017-04-15

    A series of Eu(III) doped Bi2WO6 nanoparticles were synthesized by a hydrothermal process. The obtained Bi2WO6:Eu(III) nanoparticles were characterized by XRD, SEM, luminescence spectrophotometer and DRS. The XRD and TEM results indicate that the Eu(III) doping concentration has no influence on the phase and morphology. However, the Eu(III) doping can tune the luminescence and enhance the photocatalytic activity of Bi2WO6. With the increases of Eu(3+) doping concentrations, the emission intensity of WO6(6-) group decreases nut the photocatalytic activity increases. The tunable luminescence of Bi2WO6:Eu(III) nanoparticles results from the energy transfer from WO6(6-) group to Eu(III) ion. The enhanced performance can be ascribed to efficient separation of electron and hole pairs after doping Eu(III) into the Bi2WO6 lattice.

  11. Tunable luminescence and enhanced photocatalytic activity for Eu(III) doped Bi2WO6 nanoparticles

    NASA Astrophysics Data System (ADS)

    Gu, Haidong; Yu, Lei; Wang, Juan; Ni, Min; Liu, Tingting; Chen, Feng

    2017-04-01

    A series of Eu(III) doped Bi2WO6 nanoparticles were synthesized by a hydrothermal process. The obtained Bi2WO6:Eu(III) nanoparticles were characterized by XRD, SEM, luminescence spectrophotometer and DRS. The XRD and TEM results indicate that the Eu(III) doping concentration has no influence on the phase and morphology. However, the Eu(III) doping can tune the luminescence and enhance the photocatalytic activity of Bi2WO6. With the increases of Eu3 + doping concentrations, the emission intensity of WO66 - group decreases nut the photocatalytic activity increases. The tunable luminescence of Bi2WO6:Eu(III) nanoparticles results from the energy transfer from WO66 - group to Eu(III) ion. The enhanced performance can be ascribed to efficient separation of electron and hole pairs after doping Eu(III) into the Bi2WO6 lattice.

  12. Synthesis of catalytically active form III ribulose 1,5-bisphosphate carboxylase/oxygenase in archaea.

    PubMed

    Finn, Michael W; Tabita, F Robert

    2003-05-01

    Ribulose 1,5 bisphosphate carboxylase/oxygenase (RubisCO) catalyzes the biological reduction and assimilation of carbon dioxide gas to organic carbon; it is the key enzyme responsible for the bulk of organic matter found on earth. Until recently it was believed that there are only two forms of RubisCO, form I and form II. However, the recent completion of several genome-sequencing projects uncovered open reading frames resembling RubisCO in the third domain of life, the archaea. Previous work and homology comparisons suggest that these enzymes represent a third form of RubisCO, form III. While earlier work indicated that two structurally distinct recombinant archaeal RubisCO proteins catalyzed bona fide RubisCO reactions, it was not established that the rbcL genes of anaerobic archaea can be transcribed and translated to an active enzyme in the native organisms. In this report, it is shown not only that Methanococcus jannaschii, Archaeoglobus fulgidus, Methanosarcina acetivorans, and Methanosarcina barkeri possess open reading frames with the residues required for catalysis but also that the RubisCO protein from these archaea accumulates in an active form under normal growth conditions. In addition, the form III RubisCO gene (rbcL) from M. acetivorans was shown to complement RubisCO deletion strains of Rhodobacter capsulatus and Rhodobacter sphaeroides under both photoheterotrophic and photoautotrophic growth conditions. These studies thus indicate for the first time that archaeal form III RubisCO functions in a physiologically significant fashion to fix CO(2). Furthermore, recombinant M. jannaschii, M. acetivorans, and A. fulgidus RubisCO possess unique properties with respect to quaternary structure, temperature optima, and activity in the presence of molecular oxygen compared to the previously described Thermococcus kodakaraensis and halophile proteins.

  13. Solar Energy Project, Activities: Biology.

    ERIC Educational Resources Information Center

    Tullock, Bruce, Ed.; And Others

    This guide contains lesson plans and outlines of science activities which present concepts of solar energy in the context of biology experiments. Each unit presents an introduction; objectives; skills and knowledge needed; materials; methods; questions; recommendations for further work; and a teacher information sheet. The teacher information…

  14. Health Activities Project (HAP), Trial Edition II.

    ERIC Educational Resources Information Center

    Buller, Dave; And Others

    Contained within this Health Activities Project (HAP) trial edition (set II) are a teacher information folio and numerous student activity folios which center around the idea that students in grades 5-8 can control their own health and safety. Each student folio is organized into a Synopsis, Health Background, Materials, Setting Up, and Activities…

  15. Absolute stereochemistry of fungal beauveriolide III and ACAT inhibitory activity of four stereoisomers.

    PubMed

    Ohshiro, Taichi; Namatame, Ichiji; Nagai, Kenichiro; Sekiguchi, Takafumi; Doi, Takayuki; Takahashi, Takashi; Akasaka, Kazuaki; Rudel, Lawrence L; Tomoda, Hiroshi; Omura, Satoshi

    2006-09-29

    Fungal beauveriolide III (BeauIII, 1b), a cyclodepsipeptide inhibiting acyl-CoA:cholesterol acyltransferase (ACAT) and showing antiatherogenic activity in mouse models, consists of L-Phe, L-Ala, D-allo-Ile, and 3-hydroxy-4-methyloctanoic acid (HMA) moieties, but the stereochemistry of the HMA part has not until now been fully defined. To determine it, four HMA stereoisomers were synthesized and labeled with (S)-(+)-2-(anthracene-2,3-dicarboximido)-1-propyl trifluoromethane sulfonate (AP-OTf), a chiral fluorescent reagent. The derivatives were separated by HPLC and compared with the natural HMA derivative, which was thereby identified as (3S,4S)HMA in BeauIII. Furthermore, the four beauveriolide III isomers ((3S,4S)BeauIII (23a), (3R,4R)BeauIII (23b), (3R,4S)BeauIII (23c), and (3S,4R)BeauIII (23d)) were synthesized, and it was shown that all the spectral data for 23a were identical with those for natural 1b. Isomers 23a and 23d showed potent inhibitory activity of lipid droplet accumulation in macrophages, while the other two isomers caused weak inhibition. Thus, the 3S configuration of BeauIII is important for this activity. Furthermore, 23a and 23d showed rather specific inhibition against the ACAT1 isozyme.

  16. PREPARATION AIDS FOR THE DEVELOPMENT OF CATEGORY III QUALITY ASSURANCE PROJECT PLANS

    EPA Science Inventory

    Data collection activities performed for the Risk Reduction Engineering Laboratory (RREL) of the U.S. Environmental Protection Agency are divided into four categories, depending on the intended use of the data. uality Assurance (QA) Project Plans are written to ensure that projec...

  17. Extracellular assembly and activation principles of oncogenic class III receptor tyrosine kinases.

    PubMed

    Verstraete, Kenneth; Savvides, Savvas N

    2012-11-01

    Intracellular signalling cascades initiated by class III receptor tyrosine kinases (RTK-IIIs) and their cytokine ligands contribute to haematopoiesis and mesenchymal tissue development. They are also implicated in a wide range of inflammatory disorders and cancers. Recent snapshots of RTK-III ectodomains in complex with cognate cytokines have revealed timely insights into the structural determinants of RTK-III activation, evolution and pathology. Importantly, candidate 'driver' and 'passenger' mutations that have been identified in RTK-IIIs can now be collectively mapped for the first time to structural scaffolds of the corresponding RTK-III ectodomains. Such insights will generate a renewed interest in dissecting the mechanistic effects of such mutations and their therapeutic relevance.

  18. Galleria mellonella apolipophorin III - an apolipoprotein with anti-Legionella pneumophila activity.

    PubMed

    Zdybicka-Barabas, Agnieszka; Palusińska-Szysz, Marta; Gruszecki, Wiesław I; Mak, Paweł; Cytryńska, Małgorzata

    2014-10-01

    The greater wax moth Galleria mellonella has been exploited worldwide as an alternative model host for studying pathogenicity and virulence factors of different pathogens, including Legionella pneumophila, a causative agent of a severe form of pneumonia called Legionnaires' disease. An important role in the insect immune response against invading pathogens is played by apolipophorin III (apoLp-III), a lipid- and pathogen associated molecular pattern-binding protein able to inhibit growth of some Gram-negative bacteria, including Legionella dumoffii. In the present study, anti-L. pneumophila activity of G. mellonella apoLp-III and the effects of the interaction of this protein with L. pneumophila cells are demonstrated. Alterations in the bacteria cell surface occurring upon apoLp-III treatment, revealed by Fourier transform infrared (FTIR) spectroscopy and atomic force microscopy, are also documented. ApoLp-III interactions with purified L. pneumophila LPS, an essential virulence factor of the bacteria, were analysed using electrophoresis and immunoblotting with anti-apoLp-III antibodies. Moreover, FTIR spectroscopy was used to gain detailed information on the type of conformational changes in L. pneumophila LPS and G. mellonella apoLp-III induced by their mutual interactions. The results indicate that apoLp-III binding to components of bacterial cell envelope, including LPS, may be responsible for anti-L. pneumophila activity of G. mellonella apoLp-III.

  19. Cr(III), Fe(III) and Co(III) complexes of tetradentate (ONNO) Schiff base ligands: Synthesis, characterization, properties and biological activity

    NASA Astrophysics Data System (ADS)

    Keskioğlu, Eren; Gündüzalp, Ayla Balaban; Çete, Servet; Hamurcu, Fatma; Erk, Birgül

    2008-08-01

    A series of metal complexes were synthesized from equimolar amounts of Schiff bases: 1,4-bis[3-(2-hydroxy-1-naphthaldimine)propyl]piperazine (bappnaf) and 1,8-bis[3-(2-hydroxy-1-naphthaldimine)- p-menthane (damnaf) with metal chlorides. All of synthesized compounds were characterized by elemental analyses, spectral (UV-vis, IR, 1H- 13C NMR, LC-MS) and thermal (TGA-DTA) methods, magnetic and conductance measurements. Schiff base complexes supposed in tetragonal geometry have the general formula [M(bappnaf or damnaf)]Cl· nH 2O, where M = Cr(III), Co(III) and n = 2, 3. But also Fe(III) complexes have octahedral geometry by the coordination of two water molecules and the formula is [Fe(bappnaf or damnaf)(H 2O) 2]Cl. The changes in the selected vibration bands in FT-IR indicate that Schiff bases behave as (ONNO) tetradentate ligands and coordinate to metal ions from two phenolic oxygen atoms and two azomethine nitrogen atoms. Conductance measurements suggest 1:1 electrolytic nature of the metal complexes. The synthesized compounds except bappnaf ligand have the antimicrobial activity against the bacteria: Escherichia coli (ATCC 11230), Yersinia enterocolitica (ATCC 1501), Bacillus magaterium (RSKK 5117), Bacillus subtilis (RSKK 244), Bacillus cereus (RSKK 863) and the fungi: Candida albicans (ATCC 10239). These results have been considerably interest in piperazine derivatives due to their significant applications in antimicrobial studies.

  20. Hanford spent nuclear fuel project recommended path forward, volume III: Alternatives and path forward evaluation supporting documentation

    SciTech Connect

    Fulton, J.C.

    1994-10-01

    Volume I of the Hanford Spent Nuclear Fuel Project - Recommended Path Forward constitutes an aggressive series of projects to construct and operate systems and facilities to safely retrieve, package, transport, process, and store K Basins fuel and sludge. Volume II provided a comparative evaluation of four Alternatives for the Path Forward and an evaluation for the Recommended Path Forward. Although Volume II contained extensive appendices, six supporting documents have been compiled in Volume III to provide additional background for Volume II.

  1. Title III (PACE) Projects on Reading, 1968. ERIC/CRIER Reading Review Series, Volume 2, Bibliography 16.

    ERIC Educational Resources Information Center

    Harris, Larry A., Comp.

    Fifty-nine planning and operational grants related to reading were chosen for inclusion in this bibliography from "Pacesetters in Innovation, Fiscal Year 1968" in order to provide a complete listing of reading-related Title III (PACE) projects for 1968. Documents are listed by accession number. Each entry includes citation data, index terms, and a…

  2. Manual for Title III ESEA - PL89-10 as Amended. Guidelines for Proposed Development and Project Administration.

    ERIC Educational Resources Information Center

    Georgia State Dept. of Education, Atlanta. Office of Instructional Services.

    The Title III program of the Elementary and Secondary Education Act called PACE (Projects to Advance Creativity in Education), is designed to encourage school districts to develop imaginative solutions to educational problems; to utilize research findings more effectively; and to create, design and make intelligent use of supplementary centers and…

  3. 76 FR 6812 - Proposed Shiloh III Wind Plant Project, Solano County, CA; Proposed Habitat Conservation Plan and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-08

    ... Fish and Wildlife Service Proposed Shiloh III Wind Plant Project, Solano County, CA; Proposed Habitat... Area). The Plan Area is adjacent to existing energy-producing facilities, most notably wind turbine.../California Independent System Operator power grid. Up to 59 wind turbines would be built in the Plan...

  4. Dental Office Assisting; Glossary of Key Words. Vocational Reading Power Project, Title III, E.S.E.A.

    ERIC Educational Resources Information Center

    Kremer, Bonnie

    The glossary is one of twenty in various subject areas of vocational education designed to assist the student in vocabulary mastery for particular vocational education courses. They are part of the Vocational Reading Power Project, Title III, E.S.E.A. This glossary is for a course in dental office assisting. It is divided into two parts: one…

  5. 1967-1968 Project Reports by Faculty Members of San Joaquin Delta College: A Project under Title III, Higher Education Act of 1965.

    ERIC Educational Resources Information Center

    Bullard, Richard F., Ed.

    These 17 curriculum studies by faculty members of San Joaquin Delta Junior College were funded under Title III of The Higher Education Act of 1965. They were intended to help initiate new courses, improve existing ones, or plan for future ones. Each project report gave its objective(s), the general methods for its development and completion, the…

  6. CERAPP: Collaborative Estrogen Receptor Activity Prediction Project

    PubMed Central

    Mansouri, Kamel; Abdelaziz, Ahmed; Rybacka, Aleksandra; Roncaglioni, Alessandra; Tropsha, Alexander; Varnek, Alexandre; Zakharov, Alexey; Worth, Andrew; Richard, Ann M.; Grulke, Christopher M.; Trisciuzzi, Daniela; Fourches, Denis; Horvath, Dragos; Benfenati, Emilio; Muratov, Eugene; Wedebye, Eva Bay; Grisoni, Francesca; Mangiatordi, Giuseppe F.; Incisivo, Giuseppina M.; Hong, Huixiao; Ng, Hui W.; Tetko, Igor V.; Balabin, Ilya; Kancherla, Jayaram; Shen, Jie; Burton, Julien; Nicklaus, Marc; Cassotti, Matteo; Nikolov, Nikolai G.; Nicolotti, Orazio; Andersson, Patrik L.; Zang, Qingda; Politi, Regina; Beger, Richard D.; Todeschini, Roberto; Huang, Ruili; Farag, Sherif; Rosenberg, Sine A.; Slavov, Svetoslav; Hu, Xin; Judson, Richard S.

    2016-01-01

    Background: Humans are exposed to thousands of man-made chemicals in the environment. Some chemicals mimic natural endocrine hormones and, thus, have the potential to be endocrine disruptors. Most of these chemicals have never been tested for their ability to interact with the estrogen receptor (ER). Risk assessors need tools to prioritize chemicals for evaluation in costly in vivo tests, for instance, within the U.S. EPA Endocrine Disruptor Screening Program. Objectives: We describe a large-scale modeling project called CERAPP (Collaborative Estrogen Receptor Activity Prediction Project) and demonstrate the efficacy of using predictive computational models trained on high-throughput screening data to evaluate thousands of chemicals for ER-related activity and prioritize them for further testing. Methods: CERAPP combined multiple models developed in collaboration with 17 groups in the United States and Europe to predict ER activity of a common set of 32,464 chemical structures. Quantitative structure–activity relationship models and docking approaches were employed, mostly using a common training set of 1,677 chemical structures provided by the U.S. EPA, to build a total of 40 categorical and 8 continuous models for binding, agonist, and antagonist ER activity. All predictions were evaluated on a set of 7,522 chemicals curated from the literature. To overcome the limitations of single models, a consensus was built by weighting models on scores based on their evaluated accuracies. Results: Individual model scores ranged from 0.69 to 0.85, showing high prediction reliabilities. Out of the 32,464 chemicals, the consensus model predicted 4,001 chemicals (12.3%) as high priority actives and 6,742 potential actives (20.8%) to be considered for further testing. Conclusion: This project demonstrated the possibility to screen large libraries of chemicals using a consensus of different in silico approaches. This concept will be applied in future projects related to other

  7. Hunting the human DPP III active conformation: combined thermodynamic and QM/MM calculations.

    PubMed

    Tomić, Antonija; Tomić, Sanja

    2014-11-07

    Multiple choices of the protein active conformations in flexible metalloenzymes complicate study of their catalytic mechanism. We used three different conformations of human dipeptidyl-peptidase III (DPP III) to investigate the influence of the protein environment on ligand binding and the Zn(2+) coordination. MD simulations followed by calculations of binding free energy components accomplished for a series of DPP III substrates, both synthetic and natural, revealed that binding of the β-strand shaped substrate to the five-stranded β-core of the compact DPP III form (in antiparallel fashion) is the preferred binding mode, in agreement with the experimentally determined structure of the DPP III inactive mutant-tynorphin complex (Bezerra et al., Proc. Natl. Acad. Sci. U. S. A., 2012, 109, 6525). Previously it was proposed that the catalytic mechanism of DPP III is similar to that of thermolysin, which assumes exchange of five and four coordinated Zn(2+), and activation of Zn-bound water by a nearby Glu. Our QM/MM calculations, performed for a total of 18 protein structures with different zinc ion environments, revealed that the 5-coordinated metal ion is more favourable than the 6-coordinated one in only the most compact DPP III form. Besides, in this structure E451 is H-bonded to the metal ion coordinating water. Also, our study revealed two constraints for the broad substrate specificity of DPP III. One is the possibility of the substrate adopting the β-strand shape and the other is its charged N-terminus. Altogether, we assume that the human DPP III active conformation would be the most compact form, similar to the "closed X-ray" DPP III structure.

  8. GBM heterogeneity as a function of variable epidermal growth factor receptor variant III activity

    PubMed Central

    Lindberg, Olle R.; McKinney, Andrew; Engler, Jane R.; Koshkakaryan, Gayane; Gong, Henry; Robinson, Aaron E.; Ewald, Andrew J.; Huillard, Emmanuelle; James, C. David; Molinaro, Annette M.; Shieh, Joseph T.; Phillips, Joanna J.

    2016-01-01

    Abnormal activation of the epidermal growth factor receptor (EGFR) due to a deletion of exons 2-7 of EGFR (EGFRvIII) is a common alteration in glioblastoma (GBM). While this alteration can drive gliomagenesis, tumors harboring EGFRvIII are heterogeneous. To investigate the role for EGFRvIII activation in tumor phenotype we used a neural progenitor cell-based murine model of GBM driven by EGFR signaling and generated tumor progenitor cells with high and low EGFRvIII activation, pEGFRHi and pEGFRLo. In vivo, ex vivo, and in vitro studies suggested a direct association between EGFRvIII activity and increased tumor cell proliferation, decreased tumor cell adhesion to the extracellular matrix, and altered progenitor cell phenotype. Time-lapse confocal imaging of tumor cells in brain slice cultures demonstrated blood vessel co-option by tumor cells and highlighted differences in invasive pattern. Inhibition of EGFR signaling in pEGFRHi promoted cell differentiation and increased cell-matrix adhesion. Conversely, increased EGFRvIII activation in pEGFRLo reduced cell-matrix adhesion. Our study using a murine model for GBM driven by a single genetic driver, suggests differences in EGFR activation contribute to tumor heterogeneity and aggressiveness. PMID:27738329

  9. Potent anticancer activity of photo-activated oxo-bridged diiron(III) complexes.

    PubMed

    Chanu, S Binita; Banerjee, Samya; Roy, Mithun

    2017-01-05

    Cancer-specific anticancer drugs are still an elusive goal. Using light as the temporal control to generate cytotoxic species from photo-activated prodrug in the presence or absence of molecular oxygen has shown potential application targeted chemotherapy as in photodynamic therapy (PDT). In the present work we explored the chemistry of several photo-active (μ-oxo)diiron(III) complexes of the following formulation [{Fe(μ-O) (L-his)(B)}2](ClO4)2 (1a-1c), [Fe2(μ-O)(H2O)2B4](ClO4)4 (2b, 2c) and [Fe2(μ-O)(μ-O2CMe)B4](ClO4)3 (3b, 3c), L-his = l-histidine, B is 2,2'-bipyridine, 1,10-phenanthroline (phen) and dipyrido[3,2-d:2',3'-f]quinoxaline (dpq) complexes for tumor-specific anticancer activity. Facile redox chemistry and photochemical aspects of the complexes prompted us to investigate the cytotoxic as well as the photo-activated cytotoxic properties of the complexes to the cancer cells. In the present investigation we explored the cancer-specific condition of excess concentration of H2O2 for our approach to targeted chemotherapy. Cytotoxic effect of the complexes to the cancer cells was found to be significantly higher than in normal cells indicating tumor-specific anticancer activity of the complexes. Cytotoxic effect was even more pronounced when the cancer cells treated with the complexes were exposed to the visible light (400-700 nm). There was >12 fold increase in cytotoxicity of the photoactivated complexes in cancer cells (MCF-7) in comparison to the normal cells (MCF-10a). We have defined a factor viz. cancer cell specificity factor (f) describing the targeted photochemotherapeutic effect of the complexes at their specific concentration. The factor (f) > 1 indicated the cancer cell specificity of the complexes, while f > 2.5 for the complexes under the visible light exposure suggested photodynamic effect. DCFDA assay indicated the presence of excess of ROS in the treated HeLa cells. ROS concentration was found to increase even more on visible

  10. ZnCl2-activated biochar from biogas residue facilitates aqueous As(III) removal

    NASA Astrophysics Data System (ADS)

    Xia, Dong; Tan, Fen; Zhang, Chuanpan; Jiang, Xiuli; Chen, Zheng; Li, Heng; Zheng, Yanmei; Li, Qingbiao; Wang, Yuanpeng

    2016-07-01

    Biochars prepared from biogas residue using different chemical activators were investigated for their As(III) adsorption properties. The results indicated that the original biochars did not exhibit significant As(III) adsorption. However, ZnCl2-activated biochar, which possessed the largest specific surface area, 516.67 cm2/g, and exhibited a perfectly porous texture, showed excellent performance in a 500 μgL-1 solution of As(III). Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy were utilized to identify the mechanism of As(III) adsorption by ZnCl2-activated biochar. Adsorption was found to occur mainly through ligand exchange of the hydroxyl in Zn-OH to form Zn-O-As(III), as well as through porous adsorption. As a low-cost adsorbent, the adsorption process was well fitted using a pseudo-second-order model, with an R2 > 0.993. The adsorption process was fast, requiring nearly 90 min to reach adsorption equilibrium. Batch adsorption experimental results indicated that ZnCl2-activated biochar has a maximum adsorption capacity of 27.67 mg/g at pH 7.0, and the adsorption process followed the Freundlich isotherm model well, with an R2 > 0.994. In addition, the current work demonstrated the efficiency of using ZnCl2-activated biochar adsorbent to treat As(III)-contaminated water.

  11. Antitumoral activity of trisubstituted dihydrobenzo(a)carbazoles. Part III.

    PubMed

    Segall, A; Pizzorno, M T

    2000-10-01

    Two recently synthesized, trisubstituted dihydrobenzo(a)carbazoles were investigated regarding their anti-HIV and antitumoral activity. The compounds showed some activity against melanoma, renal cancer and breast cancer cell lines.

  12. Clean Coal III Project: Blast Furnace Granular Coal Injection Project Trail 1 Report - Blast Furnace Granular Coal Injection - Results with Low Volatile Coal

    SciTech Connect

    None, None

    1997-11-01

    This report describes the first coal trial test conducted with the Blast Furnace Granular Coal Injection System at Bethlehem Steel Corporation's Burns Harbor Plant. This demonstration project is divided into three phases: Phase I - Design Phase II - Construction Phase III - Operation The design phase was conducted in 1991-1993, Construction of the facility began in August 1993 and was completed in late 1994. The coal injection facility began operating in January 1995 and Phase III began in November 1995. The Trial 1 base test on C furnace was carried out in October 1996 as a comparison period for the analysis of the operation during subsequent coal trials.

  13. Clean Coal III Project: Blast Furnace Granular Coal Injection Project Trial 1 Report - Blast Furnace Granular Coal Injection - Results with Low Volatile Coal

    SciTech Connect

    None, None

    1997-11-01

    This report describes the first coal trial test conducted with the Blast Furnace Granular Coal Injection System at Bethlehem Steel Corporation's Burns Harbor Plant. This demonstration project is divided into three phases: Phase I - Design Phase II - Construction Phase III - Operation The design phase was conducted in 1991-1993. Construction of the facility began in August 1993 and was completed in late 1994. The coal injection facility began operating in January 1995 and Phase III began in November 1995. The Trial 1 base test orI C furnace was carried out in October 1996 as a comparison period for the analysis of the operation during subsequent coal trials.

  14. An Iridium(III) Complex Inhibits JMJD2 Activities and Acts as a Potential Epigenetic Modulator.

    PubMed

    Liu, Li-Juan; Lu, Lihua; Zhong, Hai-Jing; He, Bingyong; Kwong, Daniel W J; Ma, Dik-Lung; Leung, Chung-Hang

    2015-08-27

    A novel iridium(III) complex was synthesized and evaluated for its ability to target JMJD2 enzymatic activity. The iridium(III) complex 1 can inhibit JMJD2 activity and was selective for JMJD2 activity over JARID, JMJD3, and HDAC activities. Moreover, 1 suppressed the trimethylation of the p21 promoter on H3K9me3 and interrupted the JMJD2D-H3K9me3 interactions in human cells, suggesting that it could act as an epigenetic modulator. To our knowledge, 1 represents the first metal-based JMJD2 inhibitor reported in the literature.

  15. Induction of polyclonal B cell activation and differentiation by the AIDS retrovirus (HTLV-III/LAV)

    SciTech Connect

    Higgins, S.E.; Schnittman, S.M.; Lane, H.C.; Folks, T.; Koenig, S.; Fauci, A.S.

    1986-03-05

    The immune systems of individuals infected with HTLV-III/LAV are characterized by a profound defect in cellular immunity together with paradoxical polyclonal B cell activation. The present study examined the direct effects of HTLV-III/LAV on B lymphocytes. Peripheral blood B cells from healthy donors were incubated with a variety of HTLV-III/LAV isolates for 1 h and /sup 3/H-thymidine incorporation was measured at multiple time points. Responses ranged from 9000-28,000 cpm and peaked on day 4. This B cell activation was not enhanced by the addition of interleukin-2 to culture, was not synergistic with Staphylococcus aureus Cowan I, was not modulated by the addition of T lymphocytes to culture, and was not associated with B cell transformation. Supernatant Ig could first be detected in virus-activated cultures at day 4, plateaued by day 8, and yielded a mean of 12,500 ng IgG+IgM/ml/50,000 B cells. Thus, HTLV-III/LAV is a potent T cell independent B cell mitogen capable of inducing B cell activation, proliferation, and differentiation comparable in magnitude to that of the most potent B cell activators. This biological property of HTLV-III/LAV may help explain the profound polyclonal B cell activation observed in patients with AIDS and may provide investigators with another probe for investigating the mechanisms of B cell activation.

  16. Improvement of thermostability and activity of Trichoderma reesei endo-xylanase Xyn III on insoluble substrates.

    PubMed

    Matsuzawa, Tomohiko; Kaneko, Satoshi; Yaoi, Katsuro

    2016-09-01

    Trichoderma reesei Xyn III, an endo-β-1,4-xylanase belonging to glycoside hydrolase family 10 (GH10), is vital for the saccharification of xylans in plant biomass. However, its enzymatic thermostability and hydrolytic activity on insoluble substrates are low. To overcome these difficulties, the thermostability of Xyn III was improved using random mutagenesis and directed evolution, and its hydrolytic activity on insoluble substrates was improved by creating a chimeric protein. In the screening of thermostable Xyn III mutants from a random mutagenesis library, we identified two amino acid residues, Gln286 and Asn340, which are important for the thermostability of Xyn III. The Xyn III Gln286Ala/Asn340Tyr mutant showed xylanase activity even after heat treatment at 60 °C for 30 min or 50 °C for 96 h, indicating a dramatic enhancement in thermostability. In addition, we found that the addition of a xylan-binding domain (XBD) to the C-terminal of Xyn III improved its hydrolytic activity on insoluble xylan.

  17. Interaction of antithrombin III with bovine aortic segments. Role of heparin in binding and enhanced anticoagulant activity

    SciTech Connect

    Stern, D.; Nawroth, P.; Marcum, J.; Handley, D.; Kisiel, W.; Rosenberg, R.; Stern, K.

    1985-01-01

    Bovine antithrombin III (AT III) interaction with the luminal surface of bovine aortic segments with a continuous layer of endothelium was examined. Incubation of /sup 125/I-AT III with vessel segments, previously washed free of endogenous AT III, demonstrated specific, time-dependent binding to the protease inhibitor to the endothelium. Half-maximal binding was observed at an added AT III concentration of 14 nM. Binding of /sup 125/I-AT III to the vessel wall was reversible (50% dissociated in 4 min), and addition of either heparin or Factor Xa accelerated displacement of /sup 125/I-AT III from the vessel segment. Dissociation of /sup 125/I-AT III from the vessel segment in the presence of factor Xa coincided with the formation of a Factor Xa-/sup 125/I-AT III complex. Inactivation of Factor IXa and Factor Xa by AT III was facilitated in the presence of vessel segments. Pretreatment of vessel segments with highly purified Flavobacterium heparinase precluded the vessel-dependent augmentation of AT III anticoagulant activity as well as specific binding of /sup 125/I-AT III to the vessel endothelium. In contrast, pretreatment of the vessel segments with chrondroitinases (ABC or AC) had no detectable effect on /sup 125/I-AT III binding or on AT III anticoagulant activity. AT III binding to vessel segments was competitively inhibited by increasing concentration of platelet factor 4. Binding of the protease inhibitor to vessel segments was inhibited by chemical modification of AT III lysyl or tryptophan residues. These AT III derivatives retained progressive inhibitory activity. These data suggest that heparin-like molecules are present on the aortic vessel wall and mediate binding of AT III to the vessel surface, as well as enhancing the anticoagulant activity of AT III at these sites.

  18. Effects of Al(III) and nano-Al13 species on malate dehydrogenase activity.

    PubMed

    Yang, Xiaodi; Cai, Ling; Peng, Yu; Li, Huihui; Chen, Rong Fu; Shen, Ren Fang

    2011-01-01

    The effects of different aluminum species on malate dehydrogenase (MDH) activity were investigated by monitoring amperometric i-t curves for the oxidation of NADH at low overpotential using a functionalized multi-wall nanotube (MWNT) modified glass carbon electrode (GCE). The results showed that Al(III) and Al(13) can activate the enzymatic activity of MDH, and the activation reaches maximum levels as the Al(III) and Al(13) concentration increase. Our study also found that the effects of Al(III) and Al(13) on the activity of MDH depended on the pH value and aluminum speciation. Electrochemical and circular dichroism spectra methods were applied to study the effects of nano-sized aluminum compounds on biomolecules.

  19. Effects of Al(III) and Nano-Al13 Species on Malate Dehydrogenase Activity

    PubMed Central

    Yang, Xiaodi; Cai, Ling; Peng, Yu; Li, Huihui; Chen, Rong Fu; Shen, Ren Fang

    2011-01-01

    The effects of different aluminum species on malate dehydrogenase (MDH) activity were investigated by monitoring amperometric i-t curves for the oxidation of NADH at low overpotential using a functionalized multi-wall nanotube (MWNT) modified glass carbon electrode (GCE). The results showed that Al(III) and Al13 can activate the enzymatic activity of MDH, and the activation reaches maximum levels as the Al(III) and Al13 concentration increase. Our study also found that the effects of Al(III) and Al13 on the activity of MDH depended on the pH value and aluminum speciation. Electrochemical and circular dichroism spectra methods were applied to study the effects of nano-sized aluminum compounds on biomolecules. PMID:22163924

  20. Passive and active soil gas sampling at the Mixed Waste Landfill, Technical Area III, Sandia National Laboratories/New Mexico

    SciTech Connect

    McVey, M.D.; Goering, T.J.; Peace, J.L.

    1996-02-01

    The Environmental Restoration Project at Sandia National Laboratories, New Mexico is tasked with assessing and remediating the Mixed Waste Landfill in Technical Area III. The Mixed Waste Landfill is a 2.6 acre, inactive radioactive and mixed waste disposal site. In 1993 and 1994, an extensive passive and active soil gas sampling program was undertaken to identify and quantify volatile organic compounds in the subsurface at the landfill. Passive soil gas surveys identified levels of PCE, TCE, 1,1, 1-TCA, toluene, 1,1,2-trichlorotrifluoroethane, dichloroethyne, and acetone above background. Verification by active soil gas sampling confirmed concentrations of PCE, TCE, 1,1,1-TCA, and 1,1,2-trichloro-1,2,2-trifluoroethane at depths of 10 and 30 feet below ground surface. In addition, dichlorodifluoroethane and trichlorofluoromethane were detected during active soil gas sampling. All of the volatile organic compounds detected during the active soil gas survey were present in the low ppb range.

  1. Acceptor impurity activation in III-nitride light emitting diodes

    SciTech Connect

    Römer, Friedhard Witzigmann, Bernd

    2015-01-12

    In this work, the role of the acceptor doping and the acceptor activation and its impact on the internal quantum efficiency (IQE) of a Gallium Nitride (GaN) based multi-quantum well light emitting diode is studied by microscopic simulation. Acceptor impurities in GaN are subject to a high activation energy which depends on the presence of proximate dopant atoms and the electric field. A combined model for the dopant ionization and activation barrier reduction has been developed and implemented in a semiconductor carrier transport simulator. By model calculations, we demonstrate the impact of the acceptor activation mechanisms on the decay of the IQE at high current densities, which is known as the efficiency droop. A major contributor to the droop is the electron leakage which is largely affected by the acceptor doping.

  2. On Shape Normalization for Non-Uniformly Active Catalyst Pellets. III.

    DTIC Science & Technology

    1981-10-01

    ON SHAPE NORMALIZATION FOR NON-UNIFORMLY ACTIVE CATALYST PELLET--ETC(U) OCT Al M4 MORBIDELLI . A VARMA DAA629 RI C 001 UNCLASSIFIED MRC-TSR-2289 NL 7...RESEARCH CENTRR ON SHAPE NORMALIZATION FOR NON-UNIFORMLY ACTIVE CATALYST PELLETS - III M. Morbidelli and A. Varma Technical Summary Report #2289 October...lies with N4RC, and not with the authors of this report. ON SHAPE NORMALIZATION FOR NON-UNIFORMLY ACTIVE CATALYST PELLETS - III M. Morbidelli and A

  3. The magic school bus TV project. Final technical performance report, July 1, 1992--July 31, 1995. Season III

    SciTech Connect

    1997-03-01

    For Season III, our goal has been to produce 13 new episodes that can optimally benefit from our research and experience in developing this unique series. With a total of 39 episodes, MSB`s third season will move the production from a weekly to a daily series, airing Monday to Friday and Sundays on PBS. As we delivered the 13 Season II episodes to PBS during fall of 1995, we also completed 13 more scripts for Season III production. During the remaining portion of Season III, these 13 scripts are being animated and prepared for delivery to PBS for an October 7th, 1996 launch date. Based on staggered phases of production used since the project`s inception, the development of science topics and show scripting for Season IV occurs at the same time that Season III shows are in animation and post-production. Thus, topics for Season IV shows are being selected, and science research and scripting have also begun during this time period. Both the National Science Foundation and PBS have made a commitment to Season IV, and a proposal has been submitted to Microsoft for consideration.

  4. Project WILD K-12 Curriculum and Activity Guide

    ERIC Educational Resources Information Center

    Council for Environmental Education, 2011

    2011-01-01

    The "Project WILD K-12 Curriculum and Activity Guide" focuses on wildlife and habitat. It is organized in topic units and is based on the Project WILD conceptual framework. Because these activities are designed for integration into existing courses of study, instructors may use one or many Project WILD activities or the entire set of activities…

  5. Fighting Fenton Chemistry: A Highly Active Iron(III) Tetracarbene Complex in Epoxidation Catalysis.

    PubMed

    Kück, Jens W; Anneser, Markus R; Hofmann, Benjamin; Pöthig, Alexander; Cokoja, Mirza; Kühn, Fritz E

    2015-12-07

    Organometallic Fe complexes with exceptionally high activities in homogeneous epoxidation catalysis are reported. The compounds display Fe(II) and Fe(III) oxidation states and bear a tetracarbene ligand. The more active catalyst exhibits activities up to 183 000 turnovers per hour at room temperature and turnover numbers of up to 4300 at -30 °C. For the Fe(III) complex, a decreased Fenton-type reactivity is observed compared with Fe(II) catalysts reported previously as indicated by a substantially lower H2 O2 decomposition and higher (initial) turnover frequencies. The dependence of the catalyst performance on the catalyst loading, substrate, water addition, and the oxidant is investigated. Under all applied conditions, the advantageous nature of the use of the Fe(III) complex is evident.

  6. Helioseismology of pre-emerging active regions. III. Statistical analysis

    SciTech Connect

    Barnes, G.; Leka, K. D.; Braun, D. C.; Birch, A. C.

    2014-05-01

    The subsurface properties of active regions (ARs) prior to their appearance at the solar surface may shed light on the process of AR formation. Helioseismic holography has been applied to samples taken from two populations of regions on the Sun (pre-emergence and without emergence), each sample having over 100 members, that were selected to minimize systematic bias, as described in Paper I. Paper II showed that there are statistically significant signatures in the average helioseismic properties that precede the formation of an AR. This paper describes a more detailed analysis of the samples of pre-emergence regions and regions without emergence based on discriminant analysis. The property that is best able to distinguish the populations is found to be the surface magnetic field, even a day before the emergence time. However, after accounting for the correlations between the surface field and the quantities derived from helioseismology, there is still evidence of a helioseismic precursor to AR emergence that is present for at least a day prior to emergence, although the analysis presented cannot definitively determine the subsurface properties prior to emergence due to the small sample sizes.

  7. Connective tissue-activating peptide III (CTAP-III): cloning the synthetic gene and characterization of the protein expressed in E. coli

    SciTech Connect

    Johnson, P.H.; Castor, C.W.; Walz, D.A.

    1986-05-01

    CTAP-III, an ..cap alpha..-granule protein secreted by human platelets, is known to stimulate mitogenesis, extracellular matrix synthesis, and plasminogen activator synthesis in human fibroblast cultures. From its primary sequence, a synthetic gene was constructed to code for a methionine-free derivative (Leu substituted for Met-21), then cloned and expressed in E. coli using a new expression vector containing regulatory elements of the colicin E1 operon. Partially purified recombinant CTAP-III showed a line of identity with CTAP-III by immunodiffusion against rabbit antibody to platelet-derived CTAP-III. Immunodetection of the reduced protein after SDS-PAGE electrophoresis showed a molecular weight (mobility) in agreement with the natural form. Biologic activity of rCTAP-III eluted from an antiCTAP-III immunoaffinity column was measured in human synovial cell bioassay systems. rCTAP-III stimulated synovial cell synthesis of /sup 14/C-hyaluronic acid approximately 13-fold; significant (P < 0.001) mitogenesis was also observed. These studies indicate that a sufficient quantity of bioactive peptide can be obtained for a more comprehensive study of its biologic properties.

  8. Oral contraceptives, antithrombin- III activity, and postoperative deep-vein thrombosis.

    PubMed

    Sagar, S; Stamatakis, J D; Thomas, D P; Kakkar, V V

    1976-03-06

    Deep-vein thrombosis (D.V.T.) was detected by the fibrinogen-uptake test in six out of a total of thirty-one young women undergoing emergency abdominal surgery who gave a history of recent oral contraceptive intake. In contrast, no D.V.T. developed in nineteen similar patients who were not on oral contraceptives (P less than 0-01). Plasma-antithrombin-III activity was significantly lower preoperatively in patients taking oral contraceptives; postoperative D.V.T. subsequently developed in three out of five patients with preoperative antithrombin-III activity below 50%. In seventy-eight dental patients undergoing molar extraction, antithrombin-III activity was measured before, during, and after operation. Activity fell in all patients during operation, but the fall was significantly greater in women taking oral contraceptives (P less than 0-01). The intra-operative fall in antithrombin-III activity was prevented by a small preoperative dose of subcutaneous heparin.

  9. Effectiveness of phosphate removal during anaerobic digestion of waste activated sludge by dosing iron(III).

    PubMed

    Cheng, Xiang; Wang, Jue; Chen, Bing; Wang, Yu; Liu, Jiaqi; Liu, Lubo

    2017-05-15

    Phosphate-Fe(II) precipitation induced by Fe(III) reduction during the anaerobic digestion of excess activated sludge was investigated for the removal of phosphorus and its possible recovery. The experiments were conducted with three Fe(III) sources at 35 °C and 55 °C. The results show that ferrihydrite-Fe(III) was effectively reduced during the anaerobic sludge digestion by 63% and 96% under mesophilic and thermophilic conditions, respectively. Whereas FeCl3-Fe(III) was only mesophilically reducible and the reduction of hematite-Fe(III) was unnoticeable at either temperature. Efficient precipitation of vivianite was not observed although high saturation index values, e.g., >14 (activity reduction not considered), had been reached. This reveals the complexity of vivianite precipitation in anaerobic digestion systems; for example, Fe(II) complexation and organic interference could not be ignored. With ferrihydrite amendments at a Fe/TP of 1.5, methane production from sludge digestion was reduced by 35.1% at 35 °C, and was unaffected when the digestion temperature went up to 55 °C. But, acidic FeCl3 severely inhibited the methane production and consequently the sludge biomass degradation.

  10. Project WILD Aquatic K-12 Curriculum and Activity Guide

    ERIC Educational Resources Information Center

    Council for Environmental Education, 2011

    2011-01-01

    The "Project WILD Aquatic K-12 Curriculum and Activity Guide" emphasizes aquatic wildlife and aquatic ecosystems. It is organized in topic units and is based on the Project WILD conceptual framework. Because these activities are designed for integration into existing courses of study, instructors may use one or many Project WILD Aquatic activities…

  11. Project Talented and Gifted Second Evaluation Report: ESEA Title III Region II.

    ERIC Educational Resources Information Center

    Khatena, Joe

    Presented in the annual (1974-75) evaluation of Project Talented and Gifted are results of an appraisal of over 50 student participants (10- to 12-years-old) and the project staff and resource personnel. The project is described as a 3-month institute to provide experiences in areas such as learning to use creative thinking and problem-solving…

  12. Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments.

    PubMed

    Tang, Shaogeng; Henne, W Mike; Borbat, Peter P; Buchkovich, Nicholas J; Freed, Jack H; Mao, Yuxin; Fromme, J Christopher; Emr, Scott D

    2015-12-15

    The endosomal sorting complexes required for transport (ESCRTs) constitute hetero-oligomeric machines that catalyze multiple topologically similar membrane-remodeling processes. Although ESCRT-III subunits polymerize into spirals, how individual ESCRT-III subunits are activated and assembled together into a membrane-deforming filament remains unknown. Here, we determine X-ray crystal structures of the most abundant ESCRT-III subunit Snf7 in its active conformation. Using pulsed dipolar electron spin resonance spectroscopy (PDS), we show that Snf7 activation requires a prominent conformational rearrangement to expose protein-membrane and protein-protein interfaces. This promotes the assembly of Snf7 arrays with ~30 Å periodicity into a membrane-sculpting filament. Using a combination of biochemical and genetic approaches, both in vitro and in vivo, we demonstrate that mutations on these protein interfaces halt Snf7 assembly and block ESCRT function. The architecture of the activated and membrane-bound Snf7 polymer provides crucial insights into the spatially unique ESCRT-III-mediated membrane remodeling.

  13. Studies on chemistry, spectroscopy and antioxidant activities of chromium(III)-hydrazide complexes.

    PubMed

    Shamshad, Bushra; Jamal, Rifat A; Ashiq, Uzma; Mahrooof-Tahir, Mohammad; Shaikh, Zara; Sultan, Sadaf; Khan, Khalid M

    2015-01-01

    Acid hydrazides are vital chemical entities due to their biological activities. Upon complexation with certain metal ions, their biological activities are known to be positively enhanced. The present work describes the synthesis of Cr(III)-hydrazide complexes, and their structural, spectroscopic and antioxidant properties to reveal their chemistry and biochemistry. Physical (magnetic moment, conductivity measurements), analytical (C, H, N and Cr analysis) and spectral (EI-Mass, FTIR) techniques are used for the characterization of synthesized compounds. All Cr(III)-hydrazide complexes exhibit octahedral geometry with general formula [Cr(L)2(H2O)2]Cl3. In these complexes, the hydrazide ligands are coordinated via carbonyl oxygen and terminal amino nitrogen in a bidentate fashion. All Cr(III)-hydrazide complexes were screened for in vitro diphenyldipicryl hydrazine (DPPH), superoxide dismutase and nitric oxide radical scavenging activities. Majority of the Cr(III)-hydrazide complexes were found to be more potent scavengers than their uncoordinated hydrazide ligands. This study demonstrates an interesting structure-activity relationship (SAR) which is presented here.

  14. Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments

    PubMed Central

    Tang, Shaogeng; Henne, W Mike; Borbat, Peter P; Buchkovich, Nicholas J; Freed, Jack H; Mao, Yuxin; Fromme, J Christopher; Emr, Scott D

    2015-01-01

    The endosomal sorting complexes required for transport (ESCRTs) constitute hetero-oligomeric machines that catalyze multiple topologically similar membrane-remodeling processes. Although ESCRT-III subunits polymerize into spirals, how individual ESCRT-III subunits are activated and assembled together into a membrane-deforming filament remains unknown. Here, we determine X-ray crystal structures of the most abundant ESCRT-III subunit Snf7 in its active conformation. Using pulsed dipolar electron spin resonance spectroscopy (PDS), we show that Snf7 activation requires a prominent conformational rearrangement to expose protein-membrane and protein-protein interfaces. This promotes the assembly of Snf7 arrays with ~30 Å periodicity into a membrane-sculpting filament. Using a combination of biochemical and genetic approaches, both in vitro and in vivo, we demonstrate that mutations on these protein interfaces halt Snf7 assembly and block ESCRT function. The architecture of the activated and membrane-bound Snf7 polymer provides crucial insights into the spatially unique ESCRT-III-mediated membrane remodeling. DOI: http://dx.doi.org/10.7554/eLife.12548.001 PMID:26670543

  15. Short-term and long-term treatment outcomes with Class III activator

    PubMed Central

    Ryu, Hyo-kyung; Chong, Hyun-Jeong; An, Ki-Yong

    2015-01-01

    Objective The purpose of this retrospective study was to investigate short-term and long-term skeletodental outcomes of Class III activator treatment. Methods A Class III activator treatment group (AG) comprised of 22 patients (9 boys, 13 girls) was compared with a Class III control group (CG) comprised of 17 patients (6 boys, 11 girls). The total treatment period was divided into three stages; the initial stage (T1), the post-activator treatment or post-mandibular growth peak stage (T2), and the long-term follow-up stage (T3). Cephalometric changes were evaluated statistically via the Mann-Whitney U-test and the Friedman test. Results The AG exhibited significant increases in the SNA angle, ANB angle, Wits appraisal, A point-N perpendicular, Convexity of A point, and proclination of the maxillary incisors, from T1 to T2. In the long-term follow-up (T1-T3), the AG exhibited significantly greater increases in the ANB angle, Wits appraisal, and Convexity of A point than the CG. Conclusions Favorable skeletal outcomes induced during the Class III activator treatment period were generally maintained until the long-term follow-up period of the post-mandibular growth peak stage. PMID:26445717

  16. Project WET Curriculum and Activity Guide 2.0

    ERIC Educational Resources Information Center

    Project WET Foundation, 2011

    2011-01-01

    The "Project WET Curriculum and Activity Guide 2.0" continues Project WET's dedication to 21st-century, cutting-edge water education. Now in full color, Guide 2.0 offers new activities on topics such as National Parks and storm water, fully revised and updated activities from the original Guide and the very best activities gathered from all of…

  17. Selectively lighting up two-photon photodynamic activity in mitochondria with AIE-active iridium(iii) complexes.

    PubMed

    Liu, Jiangping; Jin, Chengzhi; Yuan, Bo; Liu, Xingguo; Chen, Yu; Ji, Liangnian; Chao, Hui

    2017-02-07

    Herein a series of mitochondria-targeted AIE (aggregation-induced emission)-active Ir(iii) complexes were designed to selectively exert one-/two-photon photodynamic activities in mitochondria to address the issues which current PDT are confronted with (i.e., shallow penetration depth of routinely used irradiation; systematic toxicity associated with effective drug concentration; concentration-quenched photodynamic activity at the target, etc.).

  18. Identification of an iridium(III) complex with anti-bacterial and anti-cancer activity.

    PubMed

    Lu, Lihua; Liu, Li-juan; Chao, Wei-chieh; Zhong, Hai-Jing; Wang, Modi; Chen, Xiu-Ping; Lu, Jin-Jian; Li, Ruei-nian; Ma, Dik-Lung; Leung, Chung-Hang

    2015-09-29

    Group 9 transition metal complexes have been widely explored as therapeutic agents due to their unique geometry, their propensity to undergo ligand exchanges with biomolecules and their diverse steric and electronic properties. These metal complexes can offer distinct modes of action in living organisms compared to carbon-based molecules. In this study, we investigated the antimicrobial and anti-proliferative abilities of a series of cyclometallated iridium(III) complexes. The iridium(III) complex 1 inhibited the growth of S. aureus with MIC and MBC values of 3.60 and 7.19 μM, respectively, indicating its potent bactericidal activity. Moreover, complex 1 also exhibited cytotoxicity against a number of cancer cell lines, with particular potency against ovarian, cervical and melanoma cells. This cyclometallated iridium(III) complex is the first example of a substitutionally-inert, Group 9 organometallic compound utilized as a direct and selective inhibitor of S. aureus.

  19. Identification of an iridium(III) complex with anti-bacterial and anti-cancer activity

    PubMed Central

    Lu, Lihua; Liu, Li-Juan; Chao, Wei-chieh; Zhong, Hai-Jing; Wang, Modi; Chen, Xiu-Ping; Lu, Jin-Jian; Li, Ruei-nian; Ma, Dik-Lung; Leung, Chung-Hang

    2015-01-01

    Group 9 transition metal complexes have been widely explored as therapeutic agents due to their unique geometry, their propensity to undergo ligand exchanges with biomolecules and their diverse steric and electronic properties. These metal complexes can offer distinct modes of action in living organisms compared to carbon-based molecules. In this study, we investigated the antimicrobial and anti-proliferative abilities of a series of cyclometallated iridium(III) complexes. The iridium(III) complex 1 inhibited the growth of S. aureus with MIC and MBC values of 3.60 and 7.19 μM, respectively, indicating its potent bactericidal activity. Moreover, complex 1 also exhibited cytotoxicity against a number of cancer cell lines, with particular potency against ovarian, cervical and melanoma cells. This cyclometallated iridium(III) complex is the first example of a substitutionally-inert, Group 9 organometallic compound utilized as a direct and selective inhibitor of S. aureus. PMID:26416333

  20. Pima Cultural Background Materials for an Elementary and Secondary Education Act Title III Project.

    ERIC Educational Resources Information Center

    Brook, Weston L., Comp.; And Others

    Produced with funds under Title III of the Elementary and Secondary Education Act, this unit on Pima cultural background and Middle American civilization was designed to help Pima students achieve a sense of identity and pride in their Indian background by (1) thinking of themselves as a people with a long history and (2) learning that their…

  1. Technical Reports (Part I). End of Project Report, 1968-1971, Volume III.

    ERIC Educational Resources Information Center

    Western Nevada Regional Education Center, Lovelock.

    The pamphlets included in this volume are technical reports prepared as outgrowths of the Student Information Systems of the Western Nevada Regional Education Center (WN-REC) funded by a Title III (Elementary and Secondary Education Act) grant. These reports describe methods of interpreting the printouts from the Student Information System;…

  2. Decentralized University Studies in Economics and English/The DUNE Project--An Evaluative Project Under Sub-Project III, an Alternative Form of Distribution for Higher Education. Educational Development 1976:3.

    ERIC Educational Resources Information Center

    Dahllof, Urban

    A teaching system practiced in the Swedish DUNE project, Subproject III, offered an alternative to the usual concentrated form of higher education. An attempt was made to solve the problem of educational distribution by cooperative efforts among municipal authorities, adult education associations, and two postsecondary establishments, the…

  3. Project WET: Facilitator Handbook for Implementation of Activities in Virginia.

    ERIC Educational Resources Information Center

    Sevebeck, Kathryn P.

    This handbook features ideas for implementing Project WET activities in Virginia. Project WET activities are designed for a variety of educational programs and can be used to complement existing curricula while addressing curricular objectives and educational standards nationwide. Activities include: (1) "Life Systems"; (2)…

  4. Help with Solving Technological Problems in Project Activities

    ERIC Educational Resources Information Center

    Herold, Jean-Francois; Ginestie, Jacques

    2011-01-01

    In France, project activities figure predominantly in technology education. The general idea behind learning based on project activity is to allow the pupil to get involved in the activity in question, with the pupil tackling real situations rather than ones of an abstract nature. But too often, we notice that the pedagogical strategies used by…

  5. Lafayette Parish Cooperative Jail Project--LPCJP. Phase III Final Report.

    ERIC Educational Resources Information Center

    Lafayette Parish School Board, LA.

    A three-phase project was conducted to further the education of inmates in the Lafayette (Louisiana) Parish Correctional Center. Phase I of the project was designed to prepare inmates to be eligible to take the General Educational Development (GED) test, and/or to teach life-coping skills so that they would better function in society upon their…

  6. Atomic data from the Iron Project. XVII. Radiative transition probabilities for dipole allowed and forbidden transitions in Fe III.

    NASA Astrophysics Data System (ADS)

    Nahar, S. N.; Pradhan, A. K.

    1996-11-01

    Transition probabilities are obtained for both the dipole allowed (E1) fine structure transitions and the forbidden electric quadrupole and magnetic dipole (E2, M1) transitions in Fe III. For the E1 transitions, ab initio calculations in the close coupling (CC) approximation using the R-matrix method are carried out in LS coupling with a 49-term eigenfunction expansion for Fe IV. The fine structure components are obtained through algebraic transformation of the LS line strengths, and the oscillator strengths and A-coefficients are computed using spectroscopic energies of the observed levels. Radiative transition probabilities for 9797 fine structure E1 transitions corresponding to 1408 LS multiplets among 200 bound states of Fe III are reported. Forbidden E2 and M1 transition probabilities are computed for 362 transitions among the 34 fine structure levels of all 16 LS terms dominated by the 3d^6^ configuration using optimised configuration-interaction wavefunctions from the SUPERSTRUCTURE program in the Breit-Pauli approximation. Comparison of the present results is made with previous calculations and significant differences are found. Theoretical line ratios computed using the present E2 and M1 A-coefficients show better agreement with observations for some prominent Fe III lines in the infra-red than those using the earlier data by Garstang (1957MNRAS.117..393G). This work is carried out as part of the Iron Project to obtain accurate radiative and collisional data for the Iron group elements.

  7. Ferrous-activated persulfate oxidation of arsenic(III) and diuron in aquatic system.

    PubMed

    Zhou, Lei; Zheng, Wei; Ji, Yuefei; Zhang, Jinfeng; Zeng, Chao; Zhang, Ya; Wang, Qi; Yang, Xi

    2013-12-15

    In situ chemical oxidation (ISCO) can be an effective technology for the remediation of soil and groundwater polluted by organic and inorganic contaminants. This study investigated the oxidation of arsenic(III) (As(III)) and diuron using ferrous activated persulfate-based ISCO. The results indicated that Fe(II)/persulfate oxidation could be an effective method to oxidize As(III) and diuron. Effects of pH, S2O8(2-) and Fe(II) amounts on the destruction of As(III) and diuron were examined in batch experiments. Acidic conditions favored the removal of As(III) and diuron. Four chelating agents, citric acid (CA), Na2S2O3, diethylene triamine pentaacetic acid (DTPA) and ethylene diamine tetraacetic acid disodium (EDTA-Na2) were used in attempt to maintain the quantity of ferrous ion in solution. In our experiments, CA and Na2S2O3 were found to be more effective than DTPA and EDTA-Na2. Our results also revealed a widely practical prospect of inorganic chelating agent Na2S2O3. Hydroxyl and sulfate radical were determined to play key roles in the oxidation process by using ethanol and tertiary butanol as molecular probes. Oxidation of As(III) yielded As(V) via the electron-transfer reaction. In the oxidation process of diuron, a stepwise nucleophilic substitution of chlorine by hydroxyl and a stepwise oxidation process of the methyl on the dimethylurea group by hydroxyl and sulfate radical were proposed.

  8. Wind River Watershed Project; Volume II of III Reports F and G, 1998 Annual Report.

    SciTech Connect

    Connolly, Patrick J.

    1999-11-01

    The authors report here their on-ground restoration actions. Part 1 describes work conducted by the Underwood Conservation District (UCD) on private lands. This work involves the Stabler Cut-Bank project. Part 2 describes work conducted by the U.S. Forest Service. The Stabler Cut-Bank Project is a cooperative stream restoration effort between Bonneville Power Administration (BPA), the UCD, private landowners, the U.S. Forest Service (USFS), and the U.S. Fish and Wildlife Service (USFWS). The Stabler site was identified by UCD during stream surveys conducted in 1996 as part of a USFWS funded project aimed at initiating water quality and habitat restoration efforts on private lands in the basin. In 1997 the Wind River Watershed Council selected the project as a top priority demonstration project. The landowners were approached by the UCD and a partnership developed. Due to their expertise in channel rehabilitation, the Forest Service was consulted for the design and assisted with the implementation of the project. A portion of the initial phase of the project was funded by USFWS. However, the majority of funding (approximately 80%) has been provided by BPA and it is anticipated that additional work that is planned for the site will be conducted with BPA funds.

  9. Copenhagen Campaigners: An Active Citizens Project

    ERIC Educational Resources Information Center

    Knox, Rohan

    2010-01-01

    In the lead-up to the Copenhagen Climate talks in December 2009, Islington Council's Sustainable Schools Officer involved seven local schools in an engaging citizenship project entitled "Copenhagen Campaigners". The aim of the project was to raise pupil's awareness of this historic global event and empower them to take action on a local…

  10. Project WILD: Aquatic Education Activity Guide.

    ERIC Educational Resources Information Center

    Memphis State Univ., TN. Tennessee Administrative Software Clearinghouse.

    Project WILD is an interdisciplinary, supplementary environmental and conservation education program which emphasizes wildlife. This document is one guide developed by Project WILD with the specific purpose of focusing on aquatic wildlife, or any wild animals that depend upon aquatic environments for survival. The book contains instructional…

  11. Inspection methods for physical protection Task III review of other agencies' physical security activities for research reactors

    SciTech Connect

    1980-01-01

    In Task I of this project, the current Nuclear Regulatory Commission (NRC) position-on physical security practices and procedures at research reactors were reviewed. In the second task, a sampling of the physical security plans was presented and the three actual reactor sites described in the security plans were visited. The purpose of Task III is to review other agencies' physical security activities for research reactors. During this phase, the actions, procedures and policies of two domestic and two foreign agencies other than the NRC that relate to the research reactor community were examined. The agencies examined were: International Atomic Energy Agency; Canadian Atomic Energy Control Board; Department of Energy; and American Nuclear Insurers.

  12. Adult Dyslexia and Attention Deficit Disorder in Finland--Project DyAdd: WAIS-III cognitive profiles.

    PubMed

    Laasonen, Marja; Leppämäki, Sami; Tani, Pekka; Hokkanen, Laura

    2009-01-01

    The project Adult Dyslexia and Attention Deficit Disorder in Finland (Project DyAdd) compares adults (n = 119, 18-55 years) with dyslexia, attention-deficit/hyperactivity disorder (ADHD), dyslexia together with ADHD (comorbid), and healthy controls with neuropsychological, psychophysical, and biological methods. The focus of this article is on the Wechsler Adult Intelligence Scale-Third Edition (WAIS-III). The clinical groups performed well compared to the norms, and they did not differ from each other. However, compared to the controls, all of them were slightly poorer in their Full IQ, and of the factors, processing speed was relatively difficult for all of them. In addition to the group comparisons, a cluster analysis based on subtest scores was conducted over the clinical groups. It did not suggest a solution that would differentiate between the clinical groups. Instead, four clusters emerged: above average, average, poor perceptual organization, and poor working memory. Thus, differentiating between these clinical groups with the WAIS-III was not possible. However, all of them shared a relative difficulty in processing speed, and group-independent clusters with perceptual or memory difficulties emerged.

  13. Low molecular weight heparin restores antithrombin III activity from hyperglycemia induced alterations.

    PubMed

    Ceriello, A; Marchi, E; Palazzni, E; Quatraro, A; Giugliano, D

    1990-01-01

    Alteration of antithrombin III (ATIII) activity, glycemia level dependent, exists in diabetes mellitus. In this study the ability of a low molecular weight heparin (LMWH) (Fluxum, Alfa-Wassermann S.p.A., Bologna, Italy), as well as unfractioned héparin, to preserve ATIII activity from glucose-induced alterations, both in vitro and in vivo, is reported. The subcutaneous and intravenous LMWH and heparin administration increases basal depressed ATIII activity in diabetic patients. Heparin shows an equivalent effect on both anti-IIa and anti-Xa activity of ATIII, while LMWH is more effective in preserving the anti-Xa activity. Similarity, heparin preserves ATIII activity from hyperglycemia-induced alterations, during hyperglycemic clamp, and LMWH infusion is able to preserve a significant amount of anti-Xa activity from glucose-induced alterations. Since diabetic patients show a high incidence of thrombotic accidents, LMWH appears to be a promising innovation for the prevention of diabetic thrombophylia.

  14. Cytotoxic activity of new neodymium (III) complexes of bis-coumarins.

    PubMed

    Kostova, Irena; Manolov, Ilia; Momekov, Georgi

    2004-09-01

    Complexes of neodymium (III) with bis-coumarins: 3,3'-benzylidene-bis(4-hydroxy-2H-1-benzopyran-2-one); bis(4-hydroxy-2-oxo-2H-chromen-3-yl)-piridin-2-yl-methane; bis(4-hydroxy-2-oxo-2H-chromen-3-yl)-piridin-4-yl-methane; bis(4-hydroxy-2-oxo-2H-chromen-3-yl)-(1H-pyrazol-3-yl)-methane were synthesized by reaction of neodymium (III) salt and the ligands, in amounts equal to metal:ligand molar ratio of 1:2. The complexes were prepared by adding an aqueous solution of neodymium (III) salt to an aqueous solution of the ligand subsequently raising the pH of the mixture gradually to ca. 5.0 by adding dilute solution of sodium hydroxide. The neodymium (III) complexes with bis-coumarins were characterized by different physicochemical methods-elemental analysis, IR-, (1)H- and (13)C-NMR-spectroscopies and mass-spectral data. The spectral data of neodymium (III) complexes were interpreted on the basis of comparison with the spectra of the free ligands. This analysis showed that in the Nd (III) complexes the ligands coordinated to the metal ion through both deprotonated hydroxyl groups. On the basis of the nu(C=O) red shift observed, participation of the carbonyl groups in the coordination to the metal ion was also suggested. Cytotoxic screening by MTT assay was carried out. The complexes were tested on HL-60, HL-60/Dox and SKW-3 cell lines. The overall results from the preliminary screening program revealed that all of the new Nd (III) complexes reach 50% inhibition of the malignant cells proliferation and thus could be considered as biologically active. On the basis of the IC(50) values obtained compounds Nd(L(1))(OH).H(2)O and Nd(L(3))(OH).2H(2)O were found to exert superior activity in comparison to the remaining complexes.

  15. Iron(III)-induced activation of chloride and bromide from modeled salt pans.

    PubMed

    Wittmer, Julian; Bleicher, Sergej; Zetzsch, Cornelius

    2015-05-14

    The photochemistry of halides in sea spray aerosol, on salt pans, and on other salty surfaces leads to a formation of reactive halogen species. We investigated the photochemical formation of atomic chlorine (Cl) and bromine (Br) in the gas phase in the presence of laboratory-modeled salt pans consisting of sodium chloride doped with iron(III) chloride hexahydrate (0.5 and 2 wt %). The samples were spread on a Teflon sheet and exposed to simulated sunlight in a Teflon smog chamber in purified, humidified air in the presence of a test mixture of hydrocarbons at the ppb level to determine Cl, Br, and OH formation by the radical clock method. Driven by the photolytic reduction of Fe(III) to Fe(II), the production rates of the Fe(III)-doped NaCl salt samples (up to10(7) atoms cm(-3) s(-1)) exceeded the release of Cl above a pure NaCl sample by more than an order of magnitude in an initially O3-free environment at low NOX. In bromide-doped samples (0.5 wt % NaBr), a part of the Cl release was replaced by Br when Fe(III) was present. Additions of sodium sulfate, sodium oxalate, oxalic acid, and catechol to NaCl/FeCl3 samples were found to restrain the activation of chloride.

  16. Angiotensin III stimulates ERK1/2 mitogen-activated protein kinases and astrocyte growth in cultured rat astrocytes.

    PubMed

    Clark, Michelle A; Tran, Hsieu; Nguyen, Chinh

    2011-10-01

    Angiotensin (Ang) III is a biologically active metabolite of Ang II with similar effects and receptor binding properties as Ang II. Most Ang III studies delineate physiological effects of the peptide but, the intracellular pathways leading to the actions are unknown and are a focus of these studies. We investigated in cultured brainstem and cerebellum rat astrocytes whether Ang III stimulates ERK1/2 mitogen activated protein (MAP) kinases and astrocyte growth. Ang III significantly stimulated ERK1/2 MAP kinases in a dose- and time-dependent manner. The maximal stimulation occurred with 100 nM Ang III (2.8±0.3 and 2.3±0.1-fold over basal, in brainstem and cerebellum astrocytes, respectively). This stimulation occurred as early as 1 min, and was sustained for at least 15 min. Moreover, inhibition of the ERK1/2 MAP kinase pathway by 10 μM PD98059 attenuated Ang III-induced ERK1/2 phosphorylation. Ang III induction of ERK1/2 occurred via stimulation of the Ang AT(1) receptor since pretreatment with 10 μM Losartan, a selective AT(1) receptor blocker, prevented Ang III-induced ERK1/2 phosphorylation. The selective AT(2) Ang receptor blocker PD123319 was ineffective. Comparable to Ang II, Ang III also stimulated astrocyte growth in a concentration-dependent manner, an effect that occurred via activation of the AT(1) receptor as well. These findings suggest that Ang III has similar effects as Ang II in astrocytes since it rapidly stimulates the phosphorylation of the ERK1/2 MAP kinases and induces astrocyte proliferation through activation of the AT(1) receptor. These studies are important in establishing signaling pathways for Ang III and provide validation of the central role of Ang III.

  17. Project-Based Language Learning: An Activity Theory Analysis

    ERIC Educational Resources Information Center

    Gibbes, Marina; Carson, Lorna

    2014-01-01

    This paper reports on an investigation of project-based language learning (PBLL) in a university language programme. Learner reflections of project work were analysed through Activity Theory, where tool-mediated activity is understood as the central unit of analysis for human interaction. Data were categorised according to the components of human…

  18. Leukocyte adhesion deficiency-III is caused by mutations in KINDLIN3 affecting integrin activation

    PubMed Central

    Svensson, Lena; Howarth, Kimberley; McDowall, Alison; Patzak, Irene; Evans, Rachel; Ussar, Siegfried; Moser, Markus; Metin, Ayse; Fried, Mike; Tomlinson, Ian; Hogg, Nancy

    2009-01-01

    Integrins are the major adhesion receptors of leukocytes and platelets. β1 and β2 integrin function on leukocytes is crucial for a successful immune response and the platelet integrin αIIbβ3 initiates the process of blood clotting through binding fibrinogen1-3. Integrins on circulating cells bind poorly to their ligands but become active after ‘inside-out’ signaling through other membrane receptors4,5. Subjects with leukocyte adhesion deficiency-1 (LAD-I) do not express β2 integrins because of mutations in the gene specifying the β2 subunit, and they suffer recurrent bacterial infections6,7. Mutations affecting αIIbβ3 integrin cause the bleeding disorder termed Glanzmann’s thrombasthenia3. Subjects with LAD-III show symptoms of both LAD-I and Glanzmann’s thrombasthenia. Their hematopoietically-derived cells express β1, β2 and β3 integrins, but defective inside-out signaling causes immune deficiency and bleeding problems8. The LAD-III lesion has been attributed to a C→A mutation in the gene encoding calcium and diacylglycerol guanine nucleotide exchange factor (CALDAGGEF1; official symbol RASGRP2) specifying the CALDAG-GEF1 protein9, but we show that this change is not responsible for the LAD-III disorder. Instead, we identify mutations in the KINDLIN3 (official symbol FERMT3) gene specifying the KINDLIN-3 protein as the cause of LAD-III in Maltese and Turkish subjects. Two independent mutations result in decreased KINDLIN3 messenger RNA levels and loss of protein expression. Notably, transfection of the subjects’ lymphocytes with KINDLIN3 complementary DNA but not CALDAGGEF1 cDNA reverses the LAD-III defect, restoring integrin-mediated adhesion and migration. PMID:19234463

  19. Detailed correlation of type III radio bursts with H alpha activity. I - Active region of 22 May 1970.

    NASA Technical Reports Server (NTRS)

    Kuiper, T. B. H.; Pasachoff, J. M.

    1973-01-01

    Comparison of observations of type III impulsive radio bursts made at the Clark Lake Radio Observatory with high-spatial-resolution cinematographic observations taken at the Big Bear Solar Observatory. Use of the log-periodic radio interferometer makes it possible to localize the radio emission uniquely. This study concentrates on the particularly active region close to the limb on May 22, 1970. Sixteen of the 17 groups were associated with some H alpha activity, 11 of them with the start of such activity.

  20. Shoreline Special Education. Music and Dance. An ESEA Title III Project. Final Evaluation.

    ERIC Educational Resources Information Center

    Shoreline School District 412, Seattle, WA.

    The goals of this project were to solve the educational problems of providing an instrumental music curriculum and achieving physiological and social changes through dance for children in special education classes (K-12). Innovative methods emphasized were: 1) neuromuscular skills set to music; and, 2) a modification of the Orff methods and…

  1. Dale Avenue School Early Childhood Education Center Project. Research Bulletin Volume III, No. 2, June 1973.

    ERIC Educational Resources Information Center

    Paterson Board of Education, NJ.

    Reported are results of an evaluation of the handwriting skills of first, second, and third level students who were part of an urban early childhood education project for culturally disadvantaged children in Paterson, New Jersey. Provided is a summary of A. Gesell and F. Ilg's recommendations for handwriting instruction for kindergarten through…

  2. Comparison of the electronic and vibrational optical activity of a europium(III) complex.

    PubMed

    Wu, Tao; Hudecová, Jana; You, Xiao-Zeng; Urbanová, Marie; Bouř, Petr

    2015-04-07

    The geometry and the electronic structure of chiral lanthanide(III) complexes are traditionally probed by electronic methods, such as circularly polarised luminescence (CPL) and electronic circular dichroism (ECD) spectroscopy. The vibrational phenomena are much weaker. In the present study, however, significant enhancements of vibrational circular dichroism (VCD) and Raman optical activity (ROA) spectral intensities were observed during the formation of a chiral bipyridine-Eu(III) complex. The ten-fold enhancement of the vibrational absorption and VCD intensities was explained by a charge-transfer process and the dominant effect of the nitrate ion on the spectra. A much larger enhancement of the ROA and Raman intensities and a hundred-fold increase of the circular intensity difference (CID) ratio were explained by the resonance of the λ = 532 nm laser light with the (7)F0 → (5)D0 transitions. This phenomenon is combined with a chirality transfer, and mixing of the Raman and luminescence effects involving low-energy (7)F states of europium. The results thus indicate that the vibrational optical activity (VOA) may be a very sensitive tool for chirality detection and probing of the electronic structure of Eu(III) and other coordination compounds.

  3. Extended risk-analysis model for activities of the project.

    PubMed

    Kušar, Janez; Rihar, Lidija; Zargi, Urban; Starbek, Marko

    2013-12-01

    Project management of product/service orders has become a mode of operation in many companies. Although these are mostly cyclically recurring projects, risk management is very important for them. An extended risk-analysis model for new product/service projects is presented in this paper. Emphasis is on a solution developed in the Faculty of Mechanical Engineering in Ljubljana, Slovenia. The usual project activities risk analysis is based on evaluation of the probability that risk events occur and on evaluation of their consequences. A third parameter has been added in our model: an estimate of the incidence of risk events. On the basis of the calculated activity risk level, a project team prepares preventive and corrective measures that should be taken according to the status indicators. An important advantage of the proposed solution is that the project manager and his team members are timely warned of risk events and they can thus activate the envisaged preventive and corrective measures as necessary.

  4. DNA interaction of europium(III) complex containing 2,2'-bipyridine and its antimicrobial activity.

    PubMed

    Jahani, Shohreh; Khorasani-Motlagh, Mozhgan; Noroozifar, Meissam

    2016-01-01

    The interaction of native fish salmon DNA (FS-DNA) with [Eu(bpy)3Cl2(H2O)]Cl, where bpy is 2,2'-bipyridine, is studied at physiological pH in Tris-HCl buffer by spectroscopic methods, viscometric techniques as well as circular dichroism (CD). These experiments reveal that Eu(III) complex has interaction with FS-DNA. Moreover, binding constant and binding site size have been determined. The value of Kb has been defined 2.46 ± .02 × 10(5) M(-1). The thermodynamic parameters are calculated by Van't Hoff equation, the results show that the interaction of the complex with FS-DNA is an entropically driven phenomenon. CD spectroscopy followed by viscosity as well as fluorescence and UV--Vis measurements indicate that the complex interacts with FS-DNA via groove binding mode. Also, the synthesized Eu(III) complex has been screened for antimicrobial activities.

  5. International Project Management Committee: Overview and Activities

    NASA Technical Reports Server (NTRS)

    Hoffman, Edward

    2010-01-01

    This slide presentation discusses the purpose and composition of the International Project Management Committee (IMPC). The IMPC was established by members of 15 space agencies, companies and professional organizations. The goal of the committee is to establish a means to share experiences and best practices with space project/program management practitioners at the global level. The space agencies that are involved are: AEB, DLR, ESA, ISRO, JAXA, KARI, and NASA. The industrial and professional organizational members are Comau, COSPAR, PMI, and Thales Alenia Space.

  6. Calculus: An Active Approach with Projects.

    ERIC Educational Resources Information Center

    Hilbert, Steve; And Others

    Ithaca College, in New York, has developed and tested a projects-based first-year calculus course over the last 3 years which uses the graphs of functions and physical phenomena to illustrate and motivate the major concepts of calculus and to introduce students to mathematical modeling. The course curriculum is designed to: (1) emphasize on the…

  7. VizieR Online Data Catalog: Hubble Tarantula Treasury Project (HTTP). III. (Sabbi+, 2016)

    NASA Astrophysics Data System (ADS)

    Sabbi, E.; Lennon, D. J.; Anderson, J.; Cignoni, M.; van der Marel, R. P.; Zaritsky, D.; de Marchi, G.; Panagia, N.; Gouliermis, D. A.; Grebel, E. K.; Gallagher, J. S., III; Smith, L. J.; Sana, H.; Aloisi, A.; Tosi, M.; Evans, C. J.; Arab, H.; Boyer, M.; de Mink, S. E.; Gordon, K.; Koekemoer, A. M.; Larsen, S. S.; Ryon, J. E.; Zeidler, P.

    2016-02-01

    Hubble Tarantula Treasury Project (HTTP; HST 12939, PI Elena Sabbi + HST 12499, PI Danny Lennon) was awarded 60 orbits of HST time in cycle 20 to survey the entire Tarantula Nebula (30 Doradus), using both the UVIS and the IR channels of the Wide Field Camera 3 (WFC3), and, in parallel, the Wide Field Channel (WFC) of the Advanced Camera for Surveys (ACS). See log of the observations (from 2011 Oct 03 to 2013 Sep 17) in table 1. (2 data files).

  8. Deflocculation of Activated Sludge by the Dissimilatory Fe(III)-Reducing Bacterium Shewanella alga BrY

    PubMed Central

    Caccavo, F.; Frolund, B.; Van Ommen, Kloeke F.; Nielsen, P. H.

    1996-01-01

    The influence of microbial Fe(III) reduction on the deflocculation of autoclaved activated sludge was investigated. Fe(III) flocculated activated sludge better than Fe(II). Decreasing concentrations of Fe(III) caused an increase in sludge bulk water turbidity, while bulk water turbidity remained relatively constant over a range of Fe(II) concentrations. Cells of the dissimilatory metal-reducing bacterium Shewanella alga BrY coupled the oxidation of H(inf2) to the reduction of Fe(III) bound in sludge flocs. Cell adhesion to the Fe(III)-sludge flocs was a prerequisite for Fe(III) reduction. The reduction of Fe(III) in sludge flocs by strain BrY caused an increase in bulk water turbidity, suggesting that the sludge was deflocculated. The results of this study support previous research suggesting that microbial Fe(III) respiration may have an impact on the floc structure and colloidal chemistry of activated sludge. PMID:16535299

  9. Mononuclear Ru(III) Schiff base complexes: Synthesis, spectral, redox, catalytic and biological activity studies

    NASA Astrophysics Data System (ADS)

    Priya, N. Padma; Arunachalam, S.; Manimaran, A.; Muthupriya, D.; Jayabalakrishnan, C.

    2009-04-01

    An octahedral ruthenium(III) Schiff base complexes of the type [RuX(EPh 3)(L)] (where, X = Cl/Br; E = As/P; L = dianion of the Schiff bases derived from acetoacetanilide with o-phenylenediamine and salicylaldehyde/ o-hydroxyacetophenone/ o-vanillin/2-hydroxy-1-naphthaldehyde) have been synthesized from the reactions of equimolar reactions of [RuX 3(EPh 3) 3] and Schiff bases in benzene. The new Ru(III) Schiff base complexes have been characterized by elemental analyses, FT-IR, electronic, 1H NMR and 13C NMR spectra, EPR spectral studies, powder X-ray diffraction (XRD) and electrochemical studies. The new complexes were found to be effective catalysts for aryl-aryl coupling and the oxidation of alcohols into their corresponding carbonyl compounds, respectively, using molecular oxygen atmosphere at ambient temperature. Further, the new Ru(III) Schiff base complexes were screened for their antibacterial activity against Pseudomonas aeruginosa, Vibrio cholera, Salomonella typhi and Staphylococcus aureaus.

  10. Arsenic (III) adsorption on iron acetate coated activated alumina: thermodynamic, kinetics and equilibrium approach

    PubMed Central

    2013-01-01

    The adsorption potential of iron acetate coated activated alumina (IACAA) for removal of arsenic [As (III)] as arsenite by batch sorption technique is described. IACAA was characterized by XRD, FTIR, EDAX and SEM instruments. Percentage adsorption on IACAA was determined as a function of pH, contact time and adsorbent dose. The study revealed that the removal of As (III) was best achieved at pH =7.4. The initial As (III) concentration (0.45 mg/L) came down to less than 0.01 mg/L at contact time 90 min with adsorbent dose of 1 g/100 mL. The sorption was reasonably explained with Langmuir and Freundlich isotherms. The thermodynamic parameters such as ΔG 0 , ΔH 0 , ΔS 0 and E a were calculated in order to understand the nature of sorption process. The sorption process was found to be controlled by pseudo-second order and intraparticle diffusion models. PMID:24359995

  11. Class III peroxidases are activated in proanthocyanidin-deficient Arabidopsis thaliana seeds

    PubMed Central

    Jia, Liguo; Xu, Weifeng; Li, Wenrao; Ye, Nenghui; Liu, Rui; Shi, Lu; Bin Rahman, A. N. M. Rubaiyath; Fan, Mingshou; Zhang, Jianhua

    2013-01-01

    Background and Aims It has previously been shown that proanthocyanidins (PAs) in the seed coat of Arabidopsis thaliana have the ability to scavenge superoxide radicals (O2−). However, the physiological processess in PA-deficit seeds are not clear. It is hypothesized that there exist alternative ways in PA-deficient seeds to cope with oxidative stress. Methods The content of hydrogen peroxide (H2O2) and its relevance to the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidases was investigated in both wild-type and PA-deficit mutant seeds. A biochemical staining approach was used to detect tissue localizations of peroxidase activities in PA-deficit mutant seeds. Key Results PA-deficient mutants possess significantly lower levels of H2O2 than the wild-type, despite their higher accumulation of superoxide radicals. Screening of the key antioxidant enzymes revealed that peroxidase activity was significantly over-activated in mutant seeds. This high peroxidase activity was mainly confined to the seed coat zone. Interestingly, neither ascorbate peroxidase nor glutathione peroxidase, just the guaiacol peroxidases (class III peroxidases), was specifically activated in the seed coat. However, no significant difference in peroxidase activity was observed in embryos of either mutants or the wild-type, although gene expressions of several candidate peroxidases were down-regulated in the embryos of PA-deficient seeds. Conclusions The results suggest that enhanced class III peroxidase activity in the seed coat of PA-deficient mutants is an adaptive strategy for seed development and survival. PMID:23448691

  12. Light-Activated Protein Inhibition through Photoinduced Electron Transfer of a Ruthenium(II)–Cobalt(III) Bimetallic Complex

    PubMed Central

    Holbrook, Robert J.; Weinberg, David J.; Peterson, Mark D.; Weiss, Emily A.; Meade, Thomas J.

    2015-01-01

    We describe a mechanism of light activation that initiates protein inhibitory action of a biologically inert Co(III) Schiff base (Co(III)-sb) complex. Photoinduced electron transfer (PET) occurs from a Ru(II) bipyridal complex to a covalently attached Co(III) complex and is gated by conformational changes that occur in tens of nanoseconds. Reduction of the Co(III)-sb by PET initiates displacement of the inert axial imidazole ligands, promoting coordination to active site histidines of α-thrombin. Upon exposure to 455 nm light, the rate of ligand exchange with 4-methylimidazole, a histidine mimic, increases by approximately 5-fold, as observed by NMR spectroscopy. Similarly, the rate of α-thrombin inhibition increases over 5-fold upon irradiation. These results convey a strategy for light activation of inorganic therapeutic agents through PET utilizing redox-active metal centers. PMID:25671465

  13. Light-activated protein inhibition through photoinduced electron transfer of a ruthenium(II)–cobalt(III) bimetallic complex

    DOE PAGES

    Holbrook, Robert J.; Weinberg, David J.; Peterson, Mark D.; ...

    2015-02-11

    In this paper, we describe a mechanism of light activation that initiates protein inhibitory action of a biologically inert Co(III) Schiff base (Co(III)-sb) complex. Photoinduced electron transfer (PET) occurs from a Ru(II) bipyridal complex to a covalently attached Co(III) complex and is gated by conformational changes that occur in tens of nanoseconds. Reduction of the Co(III)-sb by PET initiates displacement of the inert axial imidazole ligands, promoting coordination to active site histidines of α-thrombin. Upon exposure to 455 nm light, the rate of ligand exchange with 4-methylimidazole, a histidine mimic, increases by approximately 5-fold, as observed by NMR spectroscopy. Similarly,more » the rate of α-thrombin inhibition increases over 5-fold upon irradiation. Finally, these results convey a strategy for light activation of inorganic therapeutic agents through PET utilizing redox-active metal centers.« less

  14. Light-activated protein inhibition through photoinduced electron transfer of a ruthenium(II)–cobalt(III) bimetallic complex

    SciTech Connect

    Holbrook, Robert J.; Weinberg, David J.; Peterson, Mark D.; Weiss, Emily A.; Meade, Thomas J.

    2015-02-11

    In this paper, we describe a mechanism of light activation that initiates protein inhibitory action of a biologically inert Co(III) Schiff base (Co(III)-sb) complex. Photoinduced electron transfer (PET) occurs from a Ru(II) bipyridal complex to a covalently attached Co(III) complex and is gated by conformational changes that occur in tens of nanoseconds. Reduction of the Co(III)-sb by PET initiates displacement of the inert axial imidazole ligands, promoting coordination to active site histidines of α-thrombin. Upon exposure to 455 nm light, the rate of ligand exchange with 4-methylimidazole, a histidine mimic, increases by approximately 5-fold, as observed by NMR spectroscopy. Similarly, the rate of α-thrombin inhibition increases over 5-fold upon irradiation. Finally, these results convey a strategy for light activation of inorganic therapeutic agents through PET utilizing redox-active metal centers.

  15. Esmeralda Energy Company, Final Scientific Technical Report, January 2008. Emigrant Slimhole Drilling Project, DOE GRED III

    SciTech Connect

    Deymonaz, John; Hulen, Jeffrey B.; Nash, Gregory D.; Schriener, Alex

    2008-01-22

    The Emigrant Slimhole Drilling Project (ESDP) was a highly successful, phased resource evaluation program designed to evaluate the commercial geothermal potential of the eastern margin of the northern Fish Lake Valley pull-apart basin in west-central Nevada. The program involved three phases: (1) Resource evaluation; (2) Drilling and resource characterization; and (3) Resource testing and assessment. Efforts included detailed geologic mapping; 3-D modeling; compilation of a GIS database; and production of a conceptual geologic model followed by the successful drilling of the 2,938 foot deep 17-31 slimhole (core hole), which encountered commercial geothermal temperatures (327⁰ F) and exhibits an increasing, conductive, temperature gradient to total depth; completion of a short injection test; and compilation of a detailed geologic core log and revised geologic cross-sections. Results of the project greatly increased the understanding of the geologic model controlling the Emigrant geothermal resource. Information gained from the 17-31 core hole revealed the existence of commercial temperatures beneath the area in the Silver Peak Core Complex which is composed of formations that exhibit excellent reservoir characteristics. Knowledge gained from the ESDP may lead to the development of a new commercial geothermal field in Nevada. Completion of the 17-31 core hole also demonstrated the cost-effectiveness of deep core drilling as an exploration tool and the unequaled value of core in understanding the geology, mineralogy, evolutional history and structural aspects of a geothermal resource.

  16. Annex III-evaluation of past and ongoing enhanced oil recovery projects

    SciTech Connect

    Not Available

    1995-02-01

    The Infill Drilling Predictive Model (IDPM) was developed by Scientific Software-Intercomp (SSI) for the Bartlesville Project Office (BPO) of the United States Department of Energy (DOE). The model and certain adaptations thereof were used in conjunction with other models to support the Interstate Oil and Gas Compact Commission`s (IOGCC) 1993 state-by-state assessment of the potential domestic reserves achievable through the application of Advanced Secondary Recovery (ASR) and Enhanced Oil Recovery (EOR) techniques. Funding for this study was provided by the DOE/BPO, which additionally provided technical support. The IDPM is a three-dimensional (stratified, five-spot), two-phase (oil and water) model which uses a minimal amount of reservoir and geologic data to generate production and recovery forecasts for ongoing waterflood and infill drilling projects. The model computes water-oil displacement and oil recovery using finite difference solutions within streamtubes. It calculates the streamtube geometries and uses a two-dimensional reservoir simulation to track fluid movement in each streamtube slice. Thus the model represents a hybrid of streamtube and numerical simulators.

  17. Healthy Activity for Secondary Students. A Collaborative Project.

    ERIC Educational Resources Information Center

    McMillen, Brooks A.; Turman, Jo

    1996-01-01

    Describes a collaborative project designed to help high school students understand healthy exercise. The project involved preservice physical education majors who acted as fitness facilitators and motivators to the high school students who selected on and off campus, moderate intensity activities. Both groups of students tracked progress and…

  18. Project Destiny: Initiating Physical Activity for Nonathletic Girls through Sport

    ERIC Educational Resources Information Center

    Kyles, Carli; Lounsbery, Monica

    2004-01-01

    The purpose of this article is to emphasize the need to develop unique physical activity and sport programs that specifically target the participation of nonathletic and nonactive girls. In addition, the authors provide an overview of an example of one such program, Project Destiny. A description of Project Destiny is provided in terms of its…

  19. Enzymatic activity and proteomic profile of class III peroxidases during sugarcane stem development.

    PubMed

    Cesarino, Igor; Araújo, Pedro; Sampaio Mayer, Juliana Lischka; Paes Leme, Adriana Franco; Mazzafera, Paulo

    2012-06-01

    Class III peroxidases are present as large multigene families in all land plants. This large number of genes together with the diversity of processes catalyzed by peroxidases suggests possible functional specialization of each isoform. However, assigning a precise role for each individual peroxidase gene has continued to be a major bottleneck. Here we investigated the enzyme activity and translational profile of class III peroxidases during stem development of sugarcane as a first step in the estimation of physiological functions of individual isoenzymes. Internodes at three different developmental stages (young, developing and mature) were divided into pith (inner tissue) and rind (outer tissue) fractions. The rind of mature internodes presented the highest enzymatic activity and thus could be considered the ideal tissue for the discovery of peroxidase gene function. In addition, activity staining of 2DE gels revealed different isoperoxidase profiles and protein expression regulation among different tissue fractions. In-gel tryptic digestion of excised spots followed by peptide sequencing by LC-MS/MS positively matched uncharacterized peroxidases in the sugarcane database SUCEST. Multiple spots matching the same peroxidase gene were found, which reflects the generation of more than one isoform from a particular gene by post-translational modifications. The identified sugarcane peroxidases appear to be monocot-specific sequences with no clear ortholog in dicot model plant Arabidopsis thaliana.

  20. The Taiwanese-American occultation survey project stellar variability. III. Detection of 58 new variable stars

    SciTech Connect

    Ishioka, R.; Wang, S.-Y.; Zhang, Z.-W.; Lehner, M. J.; Cook, K. H.; King, S.-K.; Lee, T.; Marshall, S. L.; Schwamb, M. E.; Wang, J.-H.; Wen, C.-Y.; Alcock, C.; Protopapas, P.; Axelrod, T.; Bianco, F. B.; Byun, Y.-I.; Chen, W. P.; Ngeow, C.-C.; Kim, D.-W.; Rice, J. A.

    2014-04-01

    The Taiwanese-American Occultation Survey project is designed for the detection of stellar occultations by small-size Kuiper Belt Objects, and it has monitored selected fields along the ecliptic plane by using four telescopes with a 3 deg{sup 2} field of view on the sky since 2005. We have analyzed data accumulated during 2005-2012 to detect variable stars. Sixteen fields with observations of more than 100 epochs were examined. We recovered 85 variables among a total of 158 known variable stars in these 16 fields. Most of the unrecovered variables are located in the fields observed less frequently. We also detected 58 variable stars which are not listed in the International Variable Star Index of the American Association of Variable Star Observers. These variable stars are classified as 3 RR Lyrae, 4 Cepheid, 1 δ Scuti, 5 Mira, 15 semi-regular, and 27 eclipsing binaries based on the periodicity and the profile of the light curves.

  1. Human Activity Recognition in AAL Environments Using Random Projections

    PubMed Central

    Damaševičius, Robertas; Vasiljevas, Mindaugas; Šalkevičius, Justas; Woźniak, Marcin

    2016-01-01

    Automatic human activity recognition systems aim to capture the state of the user and its environment by exploiting heterogeneous sensors attached to the subject's body and permit continuous monitoring of numerous physiological signals reflecting the state of human actions. Successful identification of human activities can be immensely useful in healthcare applications for Ambient Assisted Living (AAL), for automatic and intelligent activity monitoring systems developed for elderly and disabled people. In this paper, we propose the method for activity recognition and subject identification based on random projections from high-dimensional feature space to low-dimensional projection space, where the classes are separated using the Jaccard distance between probability density functions of projected data. Two HAR domain tasks are considered: activity identification and subject identification. The experimental results using the proposed method with Human Activity Dataset (HAD) data are presented. PMID:27413392

  2. Human Activity Recognition in AAL Environments Using Random Projections.

    PubMed

    Damaševičius, Robertas; Vasiljevas, Mindaugas; Šalkevičius, Justas; Woźniak, Marcin

    2016-01-01

    Automatic human activity recognition systems aim to capture the state of the user and its environment by exploiting heterogeneous sensors attached to the subject's body and permit continuous monitoring of numerous physiological signals reflecting the state of human actions. Successful identification of human activities can be immensely useful in healthcare applications for Ambient Assisted Living (AAL), for automatic and intelligent activity monitoring systems developed for elderly and disabled people. In this paper, we propose the method for activity recognition and subject identification based on random projections from high-dimensional feature space to low-dimensional projection space, where the classes are separated using the Jaccard distance between probability density functions of projected data. Two HAR domain tasks are considered: activity identification and subject identification. The experimental results using the proposed method with Human Activity Dataset (HAD) data are presented.

  3. Two tridentate Schiff base ligands and their mononuclear cobalt (III) complexes: Synthesis, characterization, antibacterial and antifungal activities.

    PubMed

    Gungor, Elif; Celen, Selma; Azaz, Dilek; Kara, Hulya

    2012-08-01

    Two Schiff base ligands (HL1, HL2) and their Co(III) complexes, [Co(HL1)(L1)] (1) and [Co(HL2)(L2)] (2) [where HL1=2-((E)-(2-hydroxyethylimino)methyl)-4-chlorophenol and HL2=2-((E)-(2-hydroxyethylimino)methyl)-4-bromophenol] were synthesized and characterized using spectroscopic methods. The crystal structures of 1 and 2 have been re-determined by single crystal diffraction at 100K. The ligands and their Co(III) complexes were screened for antibacterial and antifungal activities by the disc diffusion, microdilution broth and single spore culture techniques. The antimicrobial activity of the Co(III) complexes and the free ligands exhibit antimicrobial properties and the Co(III) complexes show enhanced inhibitory activity compared with their parent ligand.

  4. It's More than an Art Project. Activity.

    ERIC Educational Resources Information Center

    Wright, Phyllis; Wright, Thomas

    1998-01-01

    Shows how a first-grade class explored the activities that are completed by people who design products, develop production systems, use materials and machines to make goods, and market the output from manufacturing plants. (JOW)

  5. High chemiluminescence activity of an Fe(III)-TAML activator in aqueous-organic media and its use in the determination of organic peroxides.

    PubMed

    Demiyanova, Alexandra S; Sakharov, Ivan Yu

    2015-05-07

    High activity of Fe(III)-TAML, peroxidase mimic, upon the catalytic oxidation of luminol in aqueous-organic media (ethanol, isopropanol and acetonitrile) was determined. Using Fe(III)-TAML the sensitive chemiluminescence assays for the determination of benzoyl peroxide and tert-butyl hydroperoxide in the presence of organic solvents were performed.

  6. Nanocrystalline brookite with enhanced stability and photocatalytic activity: influence of lanthanum(III) doping.

    PubMed

    Perego, Céline; Wang, Yu-Heng; Durupthy, Olivier; Cassaignon, Sophie; Revel, Renaud; Jolivet, Jean-Pierre

    2012-02-01

    Metastable TiO(2) polymorphs are more promising materials than rutile for specific applications such as photocatalysis or catalysis support. This was clearly demonstrated for the anatase phase but still under consideration for brookite, which is difficult to obtain as pure phase. Moreover, the surface doping of anatase with lanthanum ions is known to both increase the thermal stability of the metastable phase and improve its photocatalytic activity. In this study, TiO(2) nanoparticles of almost only the brookite structure were prepared by a simple sol-gel procedure in aqueous solution. The nanoparticles were then doped with lanthanum(III) ions. The thermal stability of the nanoparticles was analyzed by X-ray diffraction and kinetic models were successfully applied to quantify phases evolutions. The presence of surface-sorbed lanthanum(III) ions increased the phase stability of at least 200 °C and this temperature shift was attributed to the selective phase stabilization of metastable TiO(2) polymorphs. Moreover, the combination of the surface doping ions and the thermal treatment induces the vanishing of the secondary anatase phase, and the photocatalytic tests on the doped brookite nanoparticles demonstrated that the doping increased photocatalytic activity and that the extent depended on the duration of the sintering treatment.

  7. The ESO Slice Project (ESP) galaxy redshift survey. III. The sample

    NASA Astrophysics Data System (ADS)

    Vettolani, G.; Zucca, E.; Merighi, R.; Mignoli, M.; Proust, D.; Zamorani, G.; Cappi, A.; Guzzo, L.; Maccagni, D.; Ramella, M.; Stirpe, G. M.; Blanchard, A.; Cayatte, V.; Collins, C.; MacGillivray, H.; Maurogordato, S.; Scaramella, R.; Balkowski, C.; Chincarini, G.; Felenbok, P.

    1998-06-01

    The ESO Slice Project (ESP) is a galaxy redshift survey extending over about 23 square degrees, in a region near the South Galactic Pole. The survey is ~ 85% complete to the limiting magnitude b_J=19.4 and consists of 3342 galaxies with redshift determination. The ESP survey is intermediate between shallow, wide angle samples and very deep, one-dimensional pencil beams; the spanned volume is ~ 5 \\ 10(4) \\htre at the sensitivity peak (z ~ 0.1). In this paper we present the description of the observations and of the data reduction, the ESP redshift catalogue and the analysis of the quality of the velocity determinations. based on observations collected at the European Southern Observatory, La Silla, Chile. Table 3 is only available (and Table 2 is also available) in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsweb.u-strasbg.fr/Abstract.html

  8. Increased alpha 2-macroglobulin in diabetes: a hyperglycemia related phenomenon associated with reduced antithrombin III activity.

    PubMed

    Ceriello, A; Giugliano, D; Quatraro, A; Stante, A; Dello Russo, P; Torella, R

    1989-01-01

    Increased alpha 2-macroglobulin (alpha 2M) activity and concentration, and decreased antithrombin III (ATIII) plasma concentration are reported in diabetic subjects. In diabetes an inverse correlation between ATIII activity and blood glucose, HbA1, alpha 2M activity and alpha 2M concentration, and a direct correlation between both alpha 2M activity and alpha 2M concentration with blood glucose and HbA1 are found. Moreover, a direct correlation between alpha 2M activity and alpha 2M concentration fails. In both diabetic and normal subjects induced hyperglycemia increases alpha 2M activity and alpha 2M concentration reduces ATIII activity, while ATIII concentration is not affected. These data which show that hyperglycemia may increase alpha 2M molecule levels while altering only the biological function of ATIII, provide evidence that hyperglycemia may decrease, directly, the biological function of some proteins and may condition the levels of some risk factors for the development of diabetic complications such as alpha 2M.

  9. Subsurface Characterization and Seismic Monitoring for the Southwest Partnerships Phase III Demonstration Project at Farnsworth Field, TX

    NASA Astrophysics Data System (ADS)

    Will, R. A.; Balch, R. S.

    2015-12-01

    The Southwest Partnership on Carbon Sequestration is performing seismic based characterization and monitoring activities at an active CO2 EOR project at Farnsworth Field, Texas. CO2 is anthropogenically sourced from a fertilizer and an ethanol plant. The field has 13 CO2 injectors and has sequestered 302,982 metric tonnes of CO2 since October 2013. The field site provides an excellent laboratory for testing a range of monitoring technologies in an operating CO2 flood since planned development is sequential and allows for multiple opportunities to record zero CO2 baseline data, mid-flood data, and fully flooded data. The project is comparing and contrasting several scales of seismic technologies in order to determine best practices for large scale commercial sequestration projects. Characterization efforts include an 85 km2 3D surface seismic survey, baseline and repeat 3D VSP surveys centered on injection wells, cross-well tomography baseline and repeat surveys between injector/producer pairs, and a borehole passive seismic array to monitor induced seismicity. All surveys have contributed to detailed geologic models which were then used for fluid flow and risk assessment simulations. 3D VSP and cross-well data with repeat surveys have allowed for direct comparisons of the reservoir prior to CO2 injection and at eight months into injection, with a goal of imaging the CO2 plume as it moves away from injection wells. Additional repeat surveys at regular intervals will continue to refine the plume. The goal of this work is to demonstrate seismic based technologies to monitor CO2 sequestration projects, and to contribute to best practices manuals for commercial scale CO2 sequestration projects. In this talk the seismic plan will be outlined, progress towards goals enumerated, and preliminary results from baseline and repeat seismic data will be discussed. Funding for this project is provided by the U.S. Department of Energy under Award No. DE-FC26-05NT42591.

  10. Solar Energy Project, Activities: Chemistry & Physics.

    ERIC Educational Resources Information Center

    Tullock, Bruce, Ed.; And Others

    This guide contains lesson plans and outlines of science activities which present concepts of solar energy in the context of chemistry and physics experiments. Each unit presents an introduction to the unit; objectives; required skills and knowledge; materials; method; questions; recommendations for further work; and a teacher information sheet.…

  11. Microcomputer Activities of the Special Materials Project.

    ERIC Educational Resources Information Center

    Novick, Leonard

    1983-01-01

    FILMSHARE, an interdepository loan system of educational captioned films for hearing impaired students, and BICS, a booking and inventory control system, are described. Use of these two microcomputer activities is explained to have increased the use of educational films and to have helped expand the collection as well. (CL)

  12. Solar Energy Project, Activities: General Solar Topics.

    ERIC Educational Resources Information Center

    Tullock, Bruce, Ed.; And Others

    This guide contains lesson plans and outlines of activities which introduce students to concepts and issues relating to solar energy. Lessons frequently presented in the context of solar energy as it relates to contemporary energy problems. Each unit presents an introduction; objectives; necessary skills and knowledge; materials; method;…

  13. Active Center Control of Termination by RNA Polymerase III and tRNA Gene Transcription Levels In Vivo

    PubMed Central

    Rijal, Keshab; Maraia, Richard J.

    2016-01-01

    The ability of RNA polymerase (RNAP) III to efficiently recycle from termination to reinitiation is critical for abundant tRNA production during cellular proliferation, development and cancer. Yet understanding of the unique termination mechanisms used by RNAP III is incomplete, as is its link to high transcription output. We used two tRNA-mediated suppression systems to screen for Rpc1 mutants with gain- and loss- of termination phenotypes in S. pombe. 122 point mutation mutants were mapped to a recently solved 3.9 Å structure of yeast RNAP III elongation complex (EC); they cluster in the active center bridge helix and trigger loop, as well as the pore and funnel, the latter of which indicate involvement of the RNA cleavage domain of the C11 subunit in termination. Purified RNAP III from a readthrough (RT) mutant exhibits increased elongation rate. The data strongly support a kinetic coupling model in which elongation rate is inversely related to termination efficiency. The mutants exhibit good correlations of terminator RT in vitro and in vivo, and surprisingly, amounts of transcription in vivo. Because assessing in vivo transcription can be confounded by various parameters, we used a tRNA reporter with a processing defect and a strong terminator. By ruling out differences in RNA decay rates, the data indicate that mutants with the RT phenotype synthesize more RNA than wild type cells, and than can be accounted for by their increased elongation rate. Finally, increased activity by the mutants appears unrelated to the RNAP III repressor, Maf1. The results show that the mobile elements of the RNAP III active center, including C11, are key determinants of termination, and that some of the mutations activate RNAP III for overall transcription. Similar mutations in spontaneous cancer suggest this as an unforeseen mechanism of RNAP III activation in disease. PMID:27518095

  14. Purification, enzymatic properties, and active site environment of a novel manganese(III)-containing acid phosphatase.

    PubMed

    Sugiura, Y; Kawabe, H; Tanaka, H; Fujimoto, S; Ohara, A

    1981-10-25

    A new manganese-containing acid phosphatase has been isolated and crystallized from sweet potato tubers. The pure enzyme contains one atom of manganese per Mr = 110,000 polypeptide and shows phosphatase activity toward various phosphate substrates. The pH optimum of the enzyme was 5.8 and the enzyme activity was inhibited by Cu2+, Zn2+, Hg2+, AsO43-, and MoO42-. This stable metalloenzyme is red-violet in color with an intense absorption band at 515 nm (epsilon - 2460). Our electronic, circular dichroism, and electron spin resonance findings strongly indicate that the Mn-valence state of the native enzyme is trivalent. When the Mn-enzyme is excited by the 5145 A line of Ar+ laser, prominent Raman lines at 1230, 1298, 1508, and 1620 cm-1 were detected. This Raman spectrum can probably be interpreted in terms of internal vibration of a coordinated tyrosine phenolate anion. The tryptophan-modified enzyme showed a positive Raman band at 370 cm-1, which is preferentially assigned to a Mn(III)-S streching mode. The modification of the Mn-enzyme by N-bromosuccinimide led to a large decrease in the fluorescence intensity of 335 nm which was dominated by its tryptophan residues within a considerable hydrophobic environment. The acid phosphatase activity was significantly decreased by the tryptophan modification. With respect to the active site donor sets, the Mn(III)-containing acid phosphatase is distinctly different from the Zn(II)-containing alkaline phosphatase. Of interest is also the appreciable similarity of some enzymatic and spectroscopic properties between the present enzyme and uteroferrin.

  15. The GROUSE project. III. Ks-band observations of the thermal emission from WASP-33b

    NASA Astrophysics Data System (ADS)

    de Mooij, E. J. W.; Brogi, M.; de Kok, R. J.; Snellen, I. A. G.; Kenworthy, M. A.; Karjalainen, R.

    2013-02-01

    Context. In recent years, day-side emission from about a dozen hot Jupiters has been detected through ground-based secondary eclipse observations in the near-infrared. These near-infrared observations are vital for determining the energy budgets of hot Jupiters, since they probe the planet's spectral energy distribution near its peak. Aims: The aim of this work is to measure the Ks-band secondary eclipse depth of WASP-33b, the first planet discovered to transit an A-type star. This planet receives the highest level of irradiation of all transiting planets discovered to date. Furthermore, its host-star shows pulsations and is classified as a low-amplitude δ Scuti. Methods: As part of our GROUnd-based Secondary Eclipse (GROUSE) project we have obtained observations of two separate secondary eclipses of WASP-33b in the Ks-band using the LIRIS instrument on the William Herschel Telescope (WHT). The telescope was significantly defocused to avoid saturation of the detector for this bright star (K ~ 7.5). To increase the stability and the cadence of the observations, they were performed in staring mode. We collected a total of 5100 and 6900 frames for the first and the second night respectively, both with an average cadence of 3.3 s. Results: On the second night the eclipse is detected at the 12 -σ level, with a measured eclipse depth of 0.244-0.020+0.027%. This eclipse depth corresponds to a brightness temperature of 3270-160+115 K. The measured brightness temperature on the second night is consistent with the expected equilibrium temperature for a planet with a very low albedo and a rapid re-radiation of the absorbed stellar light. For the other night the short out-of-eclipse baseline prevents good corrections for the stellar pulsations and systematic effects, which makes this dataset unreliable for eclipse depth measurements. This demonstrates the need of getting a sufficient out-of-eclipse baseline. Appendix A is available in electronic form at http

  16. POISSON project. III. Investigating the evolution of the mass accretion rate

    NASA Astrophysics Data System (ADS)

    Antoniucci, S.; García López, R.; Nisini, B.; Caratti o Garatti, A.; Giannini, T.; Lorenzetti, D.

    2014-12-01

    Context. As part of the Protostellar Optical-Infrared Spectral Survey On NTT (POISSON) project, we present the results of the analysis of low-resolution near-IR spectroscopic data (0.9-2.4 μm) of two samples of young stellar objects in the Lupus (52 objects) and Serpens (17 objects) star-forming clouds, with masses in the range of 0.1 to 2.0 M⊙ and ages spanning from 105 to a few 107 yr. Aims: After determining the accretion parameters of the targets by analysing their H i near-IR emission features, we added the results from the Lupus and Serpens clouds to those from previous regions (investigated in POISSON with the same methodology) to obtain a final catalogue (143 objects) of mass accretion rate values (Ṁacc) derived in a homogeneous and consistent fashion. Our final goal is to analyse how Ṁacc correlates with the stellar mass (M∗) and how it evolves in time in the whole POISSON sample. Methods: We derived the accretion luminosity (Lacc) and Ṁacc for Lupus and Serpens objects from the Brγ (Paβ in a few cases) line by using relevant empirical relationships available in the literature that connect the H i line luminosity and Lacc. To minimise the biases that arise from adopting literature data that are based on different evolutionary models and also for self-consistency, we re-derived mass and age for each source of the POISSON samples using the same set of evolutionary tracks. Results: We observe a correlation Ṁacc~M*2.2 between mass accretion rate and stellar mass, similarly to what has previously been observed in several star-forming regions. We find that the time variation of Ṁacc is roughly consistent with the expected evolution of the accretion rate in viscous disks, with an asymptotic decay that behaves as t-1.6. However, Ṁacc values are characterised by a large scatter at similar ages and are on average higher than the predictions of viscous models. Conclusions: Although part of the scattering may be related to systematics due to the

  17. Photosynthetic activity and protein overexpression found in Cr(III)-tolerant cells of the green algae Dictyosphaerium chlorelloides.

    PubMed

    Pereira, M; Bartolomé, C M; Sánchez-Fortún, S

    2014-08-01

    Chromium is an important constituent in effluents obtained from chromium plating industries. Due to the highly toxic nature of Cr(VI), attention has been shifted to less hazardous Cr(III) electroplating processes. This study evaluated aquatic toxicity of Cr(III)-containing laboratory samples representative of effluents from chromium electroplating industries, on the photosynthetic activity exhibited by both Cr(III)-sensitive (Dc1M(wt)) and tolerant (Dc1M(Cr(III)R30)) Dictyosphaerium chlorelloides strains. Additionally, selected de novo-determined peptide sequences, obtained from Dc1M(Cr(III)R30), have been analyzed to evidence the possible Cr(III) toxic mechanism involved in the resistance of these cells to high Cr(III) levels in aquatic environments. Dc1M(Cr(III)R30) strain exhibited a gross photosynthetic balance of about five times lower than that exhibited by Dc1M(wt) strain, demonstrating that Dc1M(Cr(III)R30) has a photosynthetic yield significantly lower than Dc1M(wt). SDS-PAGE of Dc1M(Cr(III)R30) samples showed the presence of at least two protein bands (23.05 and 153.46 KDa, respectively) absent in wild-type strain samples. Although it has achieved a low coincidence between the lower molecular weight band and a GTPase identified from genome of the green alga Chlamydomonas reinhardtii, none of de novo peptide sequences obtained showed a significant MS-BLAST score, so that further studies will be required.

  18. Interactive lecture demonstrations, active learning, and the ALOP project

    NASA Astrophysics Data System (ADS)

    Lakshminarayanan, Vasudevan

    2011-05-01

    There is considerable evidence from the physics education literature that traditional approaches are ineffective in teaching physics concepts. A better teaching method is to use the active learning environment, which can be created using interactive lecture demonstrations. Based on the active learning methodology and within the framework of the UNESCO mandate in physics education and introductory physics, the ALOP project (active learning in optics and photonics) was started in 2003, to provide a focus on an experimental area that is adaptable and relevant to research and educational conditions in many developing countries. This project is discussed in this paper.

  19. NASA's University Program: Active projects, fiscal year 1981

    NASA Technical Reports Server (NTRS)

    1981-01-01

    Active university R and D activities funded by NASA which contribute to mission needs are documented. Technical rather than fiscal information is emphasized. A classification of government sponsored research is included. A cross index providing access to the project description is also included.

  20. LOCAL LUMINOUS INFRARED GALAXIES. III. CO-EVOLUTION OF BLACK HOLE GROWTH AND STAR FORMATION ACTIVITY?

    SciTech Connect

    Alonso-Herrero, Almudena; Hernan-Caballero, Antonio; Pereira-Santaella, Miguel; Rieke, George H.; Diamond-Stanic, Aleksandar M.; Wang Yiping; Rigopoulou, Dimitra

    2013-03-10

    Local luminous infrared (IR) galaxies (LIRGs) have both high star formation rates (SFR) and a high AGN (Seyfert and AGN/starburst composite) incidence. Therefore, they are ideal candidates to explore the co-evolution of black hole (BH) growth and star formation (SF) activity, not necessarily associated with major mergers. Here, we use Spitzer/IRS spectroscopy of a complete volume-limited sample of local LIRGs (distances of <78 Mpc). We estimate typical BH masses of 3 Multiplication-Sign 10{sup 7} M{sub Sun} using [Ne III] 15.56 {mu}m and optical [O III] {lambda}5007 gas velocity dispersions and literature stellar velocity dispersions. We find that in a large fraction of local LIRGs, the current SFR is taking place not only in the inner nuclear {approx}1.5 kpc region, as estimated from the nuclear 11.3 {mu}m PAH luminosities, but also in the host galaxy. We next use the ratios between the SFRs and BH accretion rates (BHAR) to study whether the SF activity and BH growth are contemporaneous in local LIRGs. On average, local LIRGs have SFR to BHAR ratios higher than those of optically selected Seyferts of similar active galactic nucleus (AGN) luminosities. However, the majority of the IR-bright galaxies in the revised-Shapley-Ames Seyfert sample behave like local LIRGs. Moreover, the AGN incidence tends to be higher in local LIRGs with the lowest SFRs. All of this suggests that in local LIRGs there is a distinct IR-bright star-forming phase taking place prior to the bulk of the current BH growth (i.e., AGN phase). The latter is reflected first as a composite and then as a Seyfert, and later as a non-LIRG optically identified Seyfert nucleus with moderate SF in its host galaxy.

  1. Activities of NICT space weather project

    NASA Astrophysics Data System (ADS)

    Murata, Ken T.; Nagatsuma, Tsutomu; Watari, Shinichi; Shinagawa, Hiroyuki; Ishii, Mamoru

    NICT (National Institute of Information and Communications Technology) has been in charge of space weather forecast service in Japan for more than 20 years. The main target region of the space weather is the geo-space in the vicinity of the Earth where human activities are dominant. In the geo-space, serious damages of satellites, international space stations and astronauts take place caused by energetic particles or electromagnetic disturbances: the origin of the causes is dynamically changing of solar activities. Positioning systems via GPS satellites are also im-portant recently. Since the most significant effect of positioning error comes from disturbances of the ionosphere, it is crucial to estimate time-dependent modulation of the electron density profiles in the ionosphere. NICT is one of the 13 members of the ISES (International Space Environment Service), which is an international assembly of space weather forecast centers under the UNESCO. With help of geo-space environment data exchanging among the member nations, NICT operates daily space weather forecast service every day to provide informa-tion on forecasts of solar flare, geomagnetic disturbances, solar proton event, and radio-wave propagation conditions in the ionosphere. The space weather forecast at NICT is conducted based on the three methodologies: observations, simulations and informatics (OSI model). For real-time or quasi real-time reporting of space weather, we conduct our original observations: Hiraiso solar observatory to monitor the solar activity (solar flare, coronal mass ejection, and so on), domestic ionosonde network, magnetometer HF radar observations in far-east Siberia, and south-east Asia low-latitude ionosonde network (SEALION). Real-time observation data to monitor solar and solar-wind activities are obtained through antennae at NICT from ACE and STEREO satellites. We have a middle-class super-computer (NEC SX-8R) to maintain real-time computer simulations for solar and solar

  2. RF thermal and new cold part design studies on TTF-III input coupler for Project-X

    SciTech Connect

    Pei, Shilun; Adolphsen, Chris E.; Li, Zenghai; Solyak, Nikolay A.; Gonin, Ivan V.

    2015-05-15

    An RF power coupler is one of the key components in a superconducting (SC) linac. It provides RF power to the SC cavity and interconnects different temperature layers (1.8 K, 4.2 K, 70 K and 300 K). The TTF-III coupler is one of the most promising candidates for the High Energy (HE) linac of Project X, but it cannot meet the average power requirements because of the relatively high temperature rise on the warm inner conductor, so some design modifications will be required. In this paper, we describe our simulation studies on the copper coating thickness on the warm inner conductor with RRR values of 10 and 100. Our purpose is to rebalance the dynamic and static loads, and finally lower the temperature rise along the warm inner conductor. Additionally, to get stronger coupling, better power handling and less multipacting probability, one new cold part design was proposed using a 60 mm coaxial line; the corresponding multipacting simulation studies have also been investigated.

  3. ESCRT-III activation by parallel action of ESCRT-I/II and ESCRT-0/Bro1 during MVB biogenesis

    PubMed Central

    Tang, Shaogeng; Buchkovich, Nicholas J; Henne, W Mike; Banjade, Sudeep; Kim, Yun Jung; Emr, Scott D

    2016-01-01

    The endosomal sorting complexes required for transport (ESCRT) pathway facilitates multiple fundamental membrane remodeling events. Previously, we determined X-ray crystal structures of ESCRT-III subunit Snf7, the yeast CHMP4 ortholog, in its active and polymeric state (Tang et al., 2015). However, how ESCRT-III activation is coordinated by the upstream ESCRT components at endosomes remains unclear. Here, we provide a molecular explanation for the functional divergence of structurally similar ESCRT-III subunits. We characterize novel mutations in ESCRT-III Snf7 that trigger activation, and identify a novel role of Bro1, the yeast ALIX ortholog, in Snf7 assembly. We show that upstream ESCRTs regulate Snf7 activation at both its N-terminal core domain and the C-terminus α6 helix through two parallel ubiquitin-dependent pathways: the ESCRT-I-ESCRT-II-Vps20 pathway and the ESCRT-0-Bro1 pathway. We therefore provide an enhanced understanding for the activation of the spatially unique ESCRT-III-mediated membrane remodeling. DOI: http://dx.doi.org/10.7554/eLife.15507.001 PMID:27074665

  4. ESCRT-III activation by parallel action of ESCRT-I/II and ESCRT-0/Bro1 during MVB biogenesis.

    PubMed

    Tang, Shaogeng; Buchkovich, Nicholas J; Henne, W Mike; Banjade, Sudeep; Kim, Yun Jung; Emr, Scott D

    2016-04-13

    The endosomal sorting complexes required for transport (ESCRT) pathway facilitates multiple fundamental membrane remodeling events. Previously, we determined X-ray crystal structures of ESCRT-III subunit Snf7, the yeast CHMP4 ortholog, in its active and polymeric state (Tang et al., 2015). However, how ESCRT-III activation is coordinated by the upstream ESCRT components at endosomes remains unclear. Here, we provide a molecular explanation for the functional divergence of structurally similar ESCRT-III subunits. We characterize novel mutations in ESCRT-III Snf7 that trigger activation, and identify a novel role of Bro1, the yeast ALIX ortholog, in Snf7 assembly. We show that upstream ESCRTs regulate Snf7 activation at both its N-terminal core domain and the C-terminus α6 helix through two parallel ubiquitin-dependent pathways: the ESCRT-I-ESCRT-II-Vps20 pathway and the ESCRT-0-Bro1 pathway. We therefore provide an enhanced understanding for the activation of the spatially unique ESCRT-III-mediated membrane remodeling.

  5. Concomitant Carboxylate and Oxalate Formation From the Activation of CO2 by a Thorium(III) Complex

    PubMed Central

    Formanuik, Alasdair; Ortu, Fabrizio; Inman, Christopher J.; Kerridge, Andrew; Castro, Ludovic

    2016-01-01

    Abstract Improving our comprehension of diverse CO2 activation pathways is of vital importance for the widespread future utilization of this abundant greenhouse gas. CO2 activation by uranium(III) complexes is now relatively well understood, with oxo/carbonate formation predominating as CO2 is readily reduced to CO, but isolated thorium(III) CO2 activation is unprecedented. We show that the thorium(III) complex, [Th(Cp′′)3] (1, Cp′′={C5H3(SiMe3)2‐1,3}), reacts with CO2 to give the mixed oxalate‐carboxylate thorium(IV) complex [{Th(Cp′′)2[κ2‐O2C{C5H3‐3,3′‐(SiMe3)2}]}2(μ‐κ2:κ2‐C2O4)] (3). The concomitant formation of oxalate and carboxylate is unique for CO2 activation, as in previous examples either reduction or insertion is favored to yield a single product. Therefore, thorium(III) CO2 activation can differ from better understood uranium(III) chemistry. PMID:27714966

  6. Synthesis, Characterization, DNA Interaction, and Antitumor Activities of La (III) Complex with Schiff Base Ligand Derived from Kaempferol and Diethylenetriamine

    PubMed Central

    Wang, Qin; Huang, Yu; Zhang, Jin-Sheng; Yang, Xin-Bin

    2014-01-01

    A novel La (III) complex, [LaL(H2O)3]NO3·3H2O, with Schiff base ligand L derived from kaempferol and diethylenetriamine, has been synthesized and characterized by elemental analysis, IR, UV-visible, 1H NMR, thermogravimetric analysis, and molar conductance measurements. The fluorescence spectra, circular dichroism spectra, and viscosity measurements and gel electrophoresis experiments indicated that the ligand L and La (III) complex could bind to CT-DNA presumably via intercalative mode and the La (III) complex showed a stronger ability to bind and cleave DNA than the ligand L alone. The binding constants (Kb) were evaluated from fluorescence data and the values ranged from 0.454 to 0.659 × 105 L mol−1 and 1.71 to 17.3 × 105 L mol−1 for the ligand L and La (III) complex, respectively, in the temperature range of 298–310 K. It was also found that the fluorescence quenching mechanism of EB-DNA by ligand L and La (III) complex was a static quenching process. In comparison to free ligand L, La (III) complex exhibited enhanced cytotoxic activities against tested tumor cell lines HL-60 and HepG-2, which may correlate with the enhanced DNA binding and cleaving abilities of the La (III) complex. PMID:25371657

  7. IAU Project and Research Activity in Nepal

    NASA Astrophysics Data System (ADS)

    Gautam, Suman

    2015-08-01

    The second half of the twentieth century has witnessed a tremendous development in the field of astronomy and space exploration. The large telescope both on the land and in the orbit, using the whole range of the electromagnetic spectra from radio waves to gamma rays are extending their range of exploration, right to the edge of the observable universe, and making astounding discoveries in the process. Many large international telescope facilities and global plans are accessible to all astronomers throughout the world, providing an inexpensive entry to cutting- edge international research for developing countries.Nepal is a mountainous country it has a wide range of climatic and altitude variations which varies from an elevation of 200 meter to ≥ 4000 meter. The average temperature varies from ≥ 25 o C to ≤ 0 to 5oC. Because of these diverse weather and climatic variation there is the potential for the establishment of sophisticated observatory/ data centre and link with each other. So, the future possible opportunity of astronomy in Nepal will be discussed. Besides Education and Research activities conducted in Tribhuvan University, Nepal under the support of International Astronomical Union (IAU) will also be highlighted. The importance brought by those two workshops conducted on data simulation supported by IAU under TF1 will also be discussed which is believed to play a vital role for the promotion and development of astronomy and astrophysics in developing countries.

  8. Bile salt receptor complex activates a pathogenic type III secretion system

    PubMed Central

    Li, Peng; Rivera-Cancel, Giomar; Kinch, Lisa N; Salomon, Dor; Tomchick, Diana R; Grishin, Nick V; Orth, Kim

    2016-01-01

    Bile is an important component of the human gastrointestinal tract with an essential role in food absorption and antimicrobial activities. Enteric bacterial pathogens have developed strategies to sense bile as an environmental cue to regulate virulence genes during infection. We discovered that Vibrio parahaemolyticus VtrC, along with VtrA and VtrB, are required for activating the virulence type III secretion system 2 in response to bile salts. The VtrA/VtrC complex activates VtrB in the presence of bile salts. The crystal structure of the periplasmic domains of the VtrA/VtrC heterodimer reveals a β-barrel with a hydrophobic inner chamber. A co-crystal structure of VtrA/VtrC with bile salt, along with biophysical and mutational analysis, demonstrates that the hydrophobic chamber binds bile salts and activates the virulence network. As part of a family of conserved signaling receptors, VtrA/VtrC provides structural and functional insights into the evolutionarily conserved mechanism used by bacteria to sense their environment. DOI: http://dx.doi.org/10.7554/eLife.15718.001 PMID:27377244

  9. Chromatin-dependent regulation of RNA polymerases II and III activity throughout the transcription cycle.

    PubMed

    Jordán-Pla, Antonio; Gupta, Ishaan; de Miguel-Jiménez, Lola; Steinmetz, Lars M; Chávez, Sebastián; Pelechano, Vicent; Pérez-Ortín, José E

    2015-01-01

    The particular behaviour of eukaryotic RNA polymerases along different gene regions and amongst distinct gene functional groups is not totally understood. To cast light onto the alternative active or backtracking states of RNA polymerase II, we have quantitatively mapped active RNA polymerases at a high resolution following a new biotin-based genomic run-on (BioGRO) technique. Compared with conventional profiling with chromatin immunoprecipitation, the analysis of the BioGRO profiles in Saccharomyces cerevisiae shows that RNA polymerase II has unique activity profiles at both gene ends, which are highly dependent on positioned nucleosomes. This is the first demonstration of the in vivo influence of positioned nucleosomes on transcription elongation. The particular features at the 5' end and around the polyadenylation site indicate that this polymerase undergoes extensive specific-activity regulation in the initial and final transcription elongation phases. The genes encoding for ribosomal proteins show distinctive features at both ends. BioGRO also provides the first nascentome analysis for RNA polymerase III, which indicates that transcription of tRNA genes is poorly regulated at the individual copy level. The present study provides a novel perspective of the transcription cycle that incorporates inactivation/reactivation as an important aspect of RNA polymerase dynamics.

  10. Bile salt receptor complex activates a pathogenic type III secretion system

    SciTech Connect

    Li, Peng; Rivera-Cancel, Giomar; Kinch, Lisa N.; Salomon, Dor; Tomchick, Diana R.; Grishin, Nick V.; Orth, Kim

    2016-07-05

    Bile is an important component of the human gastrointestinal tract with an essential role in food absorption and antimicrobial activities. Enteric bacterial pathogens have developed strategies to sense bile as an environmental cue to regulate virulence genes during infection. We discovered thatVibrio parahaemolyticusVtrC, along with VtrA and VtrB, are required for activating the virulence type III secretion system 2 in response to bile salts. The VtrA/VtrC complex activates VtrB in the presence of bile salts. The crystal structure of the periplasmic domains of the VtrA/VtrC heterodimer reveals a β-barrel with a hydrophobic inner chamber. A co-crystal structure of VtrA/VtrC with bile salt, along with biophysical and mutational analysis, demonstrates that the hydrophobic chamber binds bile salts and activates the virulence network. As part of a family of conserved signaling receptors, VtrA/VtrC provides structural and functional insights into the evolutionarily conserved mechanism used by bacteria to sense their environment.

  11. Myosin III-mediated cross-linking and stimulation of actin bundling activity of Espin.

    PubMed

    Liu, Haiyang; Li, Jianchao; Raval, Manmeet H; Yao, Ningning; Deng, Xiaoying; Lu, Qing; Nie, Si; Feng, Wei; Wan, Jun; Yengo, Christopher M; Liu, Wei; Zhang, Mingjie

    2016-01-19

    Class III myosins (Myo3) and actin-bundling protein Espin play critical roles in regulating the development and maintenance of stereocilia in vertebrate hair cells, and their defects cause hereditary hearing impairments. Myo3 interacts with Espin1 through its tail homology I motif (THDI), however it is not clear how Myo3 specifically acts through Espin1 to regulate the actin bundle assembly and stabilization. Here we discover that Myo3 THDI contains a pair of repeat sequences capable of independently and strongly binding to the ankyrin repeats of Espin1, revealing an unexpected Myo3-mediated cross-linking mechanism of Espin1. The structures of Myo3 in complex with Espin1 not only elucidate the mechanism of the binding, but also reveal a Myo3-induced release of Espin1 auto-inhibition mechanism. We also provide evidence that Myo3-mediated cross-linking can further promote actin fiber bundling activity of Espin1.

  12. Control of type III secretion activity and substrate specificity by the cytoplasmic regulator PcrG

    PubMed Central

    Lee, Pei-Chung; Zmina, Stephanie Elizabeth; Stopford, Charles Morgan; Toska, Jonida; Rietsch, Arne

    2014-01-01

    Pathogenic Gram-negative bacteria use syringe-like type III secretion systems (T3SS) to inject effector proteins directly into targeted host cells. Effector secretion is triggered by host cell contact, and before contact is prevented by a set of conserved regulators. How these regulators interface with the T3SS apparatus to control secretion is unclear. We present evidence that the proton motive force (pmf) drives T3SS secretion in Pseudomonas aeruginosa, and that the cytoplasmic regulator PcrG interacts with distinct components of the T3SS apparatus to control two important aspects of effector secretion: (i) It coassembles with a second regulator (Pcr1) on the inner membrane T3SS component PcrD to prevent effectors from accessing the T3SS, and (ii) In conjunction with PscO, it controls protein secretion activity by modulating the ability of T3SS to convert pmf. PMID:24778208

  13. Quality Assurance Project Plan for Facility Effluent Monitoring Plan activities

    SciTech Connect

    Frazier, T.P.

    1994-10-20

    This Quality Assurance Project Plan addresses the quality assurance requirements for the activities associated with the Facility Effluent Monitoring Plans, which are part of the overall Hanford Site Environmental Protection Plan. This plan specifically applies to the sampling and analysis activities and continuous monitoring performed for all Facility Effluent Monitoring Plan activities conducted by Westinghouse Hanford Company. It is generic in approach and will be implemented in conjunction with the specific requirements of the individual Facility Effluent Monitoring Plans.

  14. Carbonic anhydrase III regulates peroxisome proliferator-activated receptor-{gamma}2

    SciTech Connect

    Mitterberger, Maria C.; Kim, Geumsoo; Rostek, Ursula; Levine, Rodney L.; Zwerschke, Werner

    2012-05-01

    Carbonic anhydrase III (CAIII) is an isoenzyme of the CA family. Because of its low specific anhydrase activity, physiological functions in addition to hydrating CO{sub 2} have been proposed. CAIII expression is highly induced in adipogenesis and CAIII is the most abundant protein in adipose tissues. The function of CAIII in both preadipocytes and adipocytes is however unknown. In the present study we demonstrate that adipogenesis is greatly increased in mouse embryonic fibroblasts (MEFs) from CAIII knockout (KO) mice, as demonstrated by a greater than 10-fold increase in the induction of fatty acid-binding protein-4 (FABP4) and increased triglyceride formation in CAIII{sup -/-} MEFs compared with CAIII{sup +/+} cells. To address the underlying mechanism, we investigated the expression of the two adipogenic key regulators, peroxisome proliferator-activated receptor-{gamma}2 (PPAR{gamma}2) and CCAAT/enhancer binding protein-{alpha}. We found a considerable (approximately 1000-fold) increase in the PPAR{gamma}2 expression in the CAIII{sup -/-} MEFs. Furthermore, RNAi-mediated knockdown of endogenous CAIII in NIH 3T3-L1 preadipocytes resulted in a significant increase in the induction of PPAR{gamma}2 and FABP4. When both CAIII and PPAR{gamma}2 were knocked down, FABP4 was not induced. We conclude that down-regulation of CAIII in preadipocytes enhances adipogenesis and that CAIII is a regulator of adipogenic differentiation which acts at the level of PPAR{gamma}2 gene expression. -- Highlights: Black-Right-Pointing-Pointer We discover a novel function of Carbonic anhydrase III (CAIII). Black-Right-Pointing-Pointer We show that CAIII is a regulator of adipogenesis. Black-Right-Pointing-Pointer We demonstrate that CAIII acts at the level of PPAR{gamma}2 gene expression. Black-Right-Pointing-Pointer Our data contribute to a better understanding of the role of CAIII in fat tissue.

  15. NASA Desert RATS 2011 Education Pilot Project and Classroom Activities

    NASA Technical Reports Server (NTRS)

    Gruener, J. E.; McGlone, M.; Allen, J.; Tobola, K.; Graff, P.

    2012-01-01

    The National Aeronautics and Space Administration's (NASA's) Desert Research and Technology Studies (Desert RATS) is a multi-year series of tests of hardware and operations carried out annually in the high desert of Arizona, as an analog to future exploration activities beyond low Earth orbit [1]. For the past several years, these tests have occurred in the San Francisco Volcanic Field, north of Flagstaff. For the 2011 Desert RATS season, the Exploration Systems Mission Directorate (ESMD) at NASA headquarters provided support to develop an education pilot project that would include student activities to parallel the Desert RATS mission planning and exploration activities in the classroom, and educator training sessions. The development of the pilot project was a joint effort between the NASA Johnson Space Center (JSC) Astromaterials Research and Exploration Science (ARES) Directorate and the Aerospace Education Services Project (AESP), managed at Penn State University.

  16. Characterization and Properties of Activated Carbon Prepared from Tamarind Seeds by KOH Activation for Fe(III) Adsorption from Aqueous Solution.

    PubMed

    Mopoung, Sumrit; Moonsri, Phansiri; Palas, Wanwimon; Khumpai, Sataporn

    2015-01-01

    This research studies the characterization of activated carbon from tamarind seed with KOH activation. The effects of 0.5 : 1-1.5 : 1 KOH : tamarind seed charcoal ratios and 500-700°C activation temperatures were studied. FTIR, SEM-EDS, XRD, and BET were used to characterize tamarind seed and the activated carbon prepared from them. Proximate analysis, percent yield, iodine number, methylene blue number, and preliminary test of Fe(III) adsorption were also studied. Fe(III) adsorption was carried out by 30 mL column with 5-20 ppm Fe(III) initial concentrations. The percent yield of activated carbon prepared from tamarind seed with KOH activation decreased with increasing activation temperature and impregnation ratios, which were in the range from 54.09 to 82.03 wt%. The surface functional groups of activated carbon are O-H, C=O, C-O, -CO3, C-H, and Si-H. The XRD result showed high crystallinity coming from a potassium compound in the activated carbon. The main elements found in the activated carbon by EDS are C, O, Si, and K. The results of iodine and methylene blue adsorption indicate that the pore size of the activated carbon is mostly in the range of mesopore and macropore. The average BET pore size and BET surface area of activated carbon are 67.9764 Å and 2.7167 m(2)/g, respectively. Finally, the tamarind seed based activated carbon produced with 500°C activation temperature and 1.0 : 1 KOH : tamarind seed charcoal ratio was used for Fe(III) adsorption test. It was shown that Fe(III) was adsorbed in alkaline conditions and adsorption increased with increasing Fe(III) initial concentration from 5 to 20 ppm with capacity adsorption of 0.0069-0.019 mg/g.

  17. In vivo anti-radiation activities of the Ulva pertusa polysaccharides and polysaccharide-iron(III) complex.

    PubMed

    Shi, Jinming; Cheng, Cuilin; Zhao, Haitian; Jing, Jing; Gong, Ning; Lu, Weihong

    2013-09-01

    Polysaccharides with different molecular weights were extracted from Ulva pertusa and fractionated by ultrafiltration. Iron(III) complex of the low molecular-weight U. pertusa polysaccharides were synthesized. Atomic absorption spectrum showed that the iron content of iron(III)-polysaccharide complex was 27.4%. The comparison between U. pertusa polysaccharides and their iron(III) complex showed that iron chelating altered the structural characteristics of the polysaccharides. The bioactivity analysis showed that polysaccharide with low molecular weight was more effective than polysaccharide with high molecular weight in protecting mice from radiation induced damages on bone marrow cells and immune system. Results also proved that the anti-radiation and anti-oxidative activity of iron(III) complex of low molecular-weight polysaccharides were not less than that of low molecular-weight polysaccharides.

  18. Utilizing avidity to improve antifreeze protein activity: a type III antifreeze protein trimer exhibits increased thermal hysteresis activity.

    PubMed

    Can, Özge; Holland, Nolan B

    2013-12-03

    Antifreeze proteins (AFPs) are ice growth inhibitors that allow the survival of several species living at temperatures colder than the freezing point of their bodily fluids. AFP activity is commonly defined in terms of thermal hysteresis, which is the difference observed for the solution freezing and melting temperatures. Increasing the thermal hysteresis activity of these proteins, particularly at low concentrations, is of great interest because of their wide range of potential applications. In this study, we have designed and expressed one-, two-, and three-domain antifreeze proteins to improve thermal hysteresis activity through increased binding avidity. The three-domain type III AFP yielded significantly greater activity than the one- and two-domain proteins, reaching a thermal hysteresis of >1.6 °C at a concentration of <1 mM. To elucidate the basis of this increase, the data were fit to a multidomain protein adsorption model based on the classical Langmuir isotherm. Fits of the data to the modified isotherms yield values for the equilibrium binding constants for the adsorption of AFP to ice and indicate that protein surface coverage is proportional to thermal hysteresis activity.

  19. Apelin-13 impedes foam cell formation by activating Class III PI3K/Beclin-1-mediated autophagic pathway.

    PubMed

    Yao, Feng; Lv, Yun-Cheng; Zhang, Min; Xie, Wei; Tan, Yu-Lin; Gong, Duo; Cheng, Hai-Peng; Liu, Dan; Li, Liang; Liu, Xiao-Yan; Zheng, Xi-Long; Tang, Chao-Ke

    2015-10-30

    Apelin-13, an adipokine, promotes cholesterol efflux in macrophages with antiatherosclerotic effect. Autophagy, an evolutionarily ancient response to cellular stress, has been involved in atherosclerosis. Therefore, the purpose of this study was to investigate whether apelin-13 regulates macrophage foam cell cholesterol metabolism through autophagy, and also explore the underlying mechanisms. Here, we revealed that apelin-13 decreased lipid accumulation in THP-1 derived macrophages through markedly enhancing cholesterol efflux. Our study further demonstrated that apelin-13 induced autophagy via activation of Class III phosphoinositide 3-kinase (PI3K) and Beclin-1. Inhibition of Class III PI3K and Beclin-1 suppressed the stimulatory effects of apelin-13 on autophagy activity. The present study concluded that apelin-13 reduces lipid accumulation of foam cells by activating autophagy via Class III PI3K/Beclin-1 pathway. Therefore, our results provide brand new insight about apelin-13 inhibiting foam cell formation and highlight autophagy as a promising therapeutic target in atherosclerosis.

  20. Improving Immunization Rates Using Lean Six Sigma Processes: Alliance of Independent Academic Medical Centers National Initiative III Project

    PubMed Central

    Hina-Syeda, Hussaini; Kimbrough, Christina; Murdoch, William; Markova, Tsveti

    2013-01-01

    , particularly for the residents. The hospital has completed quality improvement projects in the past; however, this project was the first in which residents were actively involved. The didactic components and experiential learning were powerfully synergistic. This and similar projects can have far-reaching implications in terms of promoting patient health and improving the quality of care delivered by the healthcare systems and teaching hospitals. PMID:24052758

  1. Synthesis, structures and urease inhibitory activity of cobalt(III) complexes with Schiff bases.

    PubMed

    Jing, Changling; Wang, Cunfang; Yan, Kai; Zhao, Kedong; Sheng, Guihua; Qu, Dan; Niu, Fang; Zhu, Hailiang; You, Zhonglu

    2016-01-15

    A series of new cobalt(III) complexes were prepared. They are [CoL(1)(py)3]·NO3 (1), [CoL(2)(bipy)(N3)]·CH3OH (2), [CoL(3)(HL(3))(N3)]·NO3 (3), and [CoL(4)(MeOH)(N3)] (4), where L(1), L(2), L(3) and L(4) are the deprotonated form of N'-(2-hydroxy-5-methoxybenzylidene)-3-methylbenzohydrazide, N'-(2-hydroxybenzylidene)-3-hydroxylbenzohydrazide, 2-[(2-dimethylaminoethylimino)methyl]-4-methylphenol, and N,N'-bis(5-methylsalicylidene)-o-phenylenediamine, respectively, py is pyridine, and bipy is 2,2'-bipyridine. The complexes were characterized by infrared and UV-Vis spectra, and single crystal X-ray diffraction. The Co atoms in the complexes are in octahedral coordination. Complexes 1 and 4 show effective urease inhibitory activities, with IC50 values of 4.27 and 0.35 μmol L(-1), respectively. Complex 2 has medium activity against urease, with IC50 value of 68.7 μmol L(-1). While complex 3 has no activity against urease. Molecular docking study of the complexes with Helicobacter pylori urease was performed.

  2. World Energy Projection System Plus (WEPS ): Global Activity Module

    EIA Publications

    2016-01-01

    The World Energy Projection System Plus (WEPS ) is a comprehensive, mid?term energy forecasting and policy analysis tool used by EIA. WEPS projects energy supply, demand, and prices by country or region, given assumptions about the state of various economies, international energy markets, and energy policies. The Global Activity Module (GLAM) provides projections of economic driver variables for use by the supply, demand, and conversion modules of WEPS . GLAM’s baseline economic projection contains the economic assumptions used in WEPS to help determine energy demand and supply. GLAM can also provide WEPS with alternative economic assumptions representing a range of uncertainty about economic growth. The resulting economic impacts of such assumptions are inputs to the remaining supply and demand modules of WEPS .

  3. Group III alcohol dehydrogenase from Pectobacterium atrosepticum: insights into enzymatic activity and organization of the metal ion-containing region.

    PubMed

    Elleuche, Skander; Fodor, Krisztian; von der Heyde, Amélie; Klippel, Barbara; Wilmanns, Matthias; Antranikian, Garabed

    2014-05-01

    NAD(P)(+)-dependent alcohol dehydrogenases (ADH) are widely distributed in all phyla. These proteins can be assigned to three nonhomologous groups of isozymes, with group III being highly diverse with regards to catalytic activity and primary structure. Members of group III ADHs share a conserved stretch of amino acid residues important for cofactor binding and metal ion coordination, while sequence identities for complete proteins are highly diverse (<20 to >90 %). A putative group III ADH PaYqhD has been identified in BLAST analysis from the plant pathogenic enterobacterium Pectobacterium atrosepticum. The PaYqhD gene was expressed in the heterologous host Escherichia coli, and the recombinant protein was purified in a two-step purification procedure to homogeneity indicating an obligate dimerization of monomers. Four conserved amino acid residues involved in metal ion coordination were substituted with alanine, and their importance for catalytic activity was confirmed by circular dichroism spectrum determination, in vitro, and growth experiments. PaYqhD exhibits optimal activity at 40 °C with short carbon chain aldehyde compounds and NADPH as cofactor indicating the enzyme to be an aldehyde reductase. No oxidative activities towards alcoholic compounds were detectable. EDTA completely inhibited catalytic activity and was fully restored by the addition of Co(2+). Activity measurements together with sequence alignments and structure analysis confirmed that PaYqhD belongs to the butanol dehydrogenase-like enzymes within group III of ADHs.

  4. Visible light photocatalytic activity induced by Rh(III) modification on the surface of BiOCl

    NASA Astrophysics Data System (ADS)

    Hu, Jinli; Wu, Xin; Huang, Caijin; Fan, Wenjie; Qiu, Xiaoqing

    2016-11-01

    Using impregnation technique, a small amount of Rh(III) clusters has been grafted on the BiOCl microflowers. The samples are characterized by X-ray diffraction, scanning electron microscopy coupled with high energy distribution X-ray, transmission electron microscopy, X-ray photoelectron spectroscopy, and UV-vis diffuse reflectance spectroscopy. The photocatalytic activities are investigated by the decomposition of gaseous acetaldehyde under irradiation of visible light. The bare BiOCl microflowers exhibit the limited visible light photocatalytic activity because of its wide band gap. After surface modification of Rh(III) clusters, the Rh(III)-BiOCl samples show an enhanced photocatalytic activity for the decomposition gaseous acetaldehyde under visible light irradiation. It is found that the Rh(III) clusters play an important role for the visible light absorption, probably through the electron transfer between Rh(III) clusters and the BiOCl, as well as the redox reaction centers for the multi-electron reduction of O2.

  5. Monitoring Project CANAL Training Activities: Parent Day Workshops for 70 Project CANAL Schools (April 18 through July 3, 1991).

    ERIC Educational Resources Information Center

    Davidson, Mary E.; Kurtz, Norman R.

    An evaluation was done to assess the participation of parents from the 70 Creating a New Approach to Learning Project (Project CANAL) schools in Project CANAL training activities. Project CANAL offered workshops for parents during the months of April and July 1991. The parent day workshops were to provide parents of participating schools with data…

  6. Observation Targeting for the Tehachapi Pass and Mid-Columbia Basin: WindSENSE Phase III Project Summary Report

    SciTech Connect

    Hanley, D

    2011-10-22

    The overall goal of this multi-phased research project known as WindSENSE is to develop an observation system deployment strategy that would improve wind power generation forecasts. The objective of the deployment strategy is to produce the maximum benefit for 1- to 6-hour ahead forecasts of wind speed at hub-height ({approx}80 m). In Phase III of the project, the focus was on the Mid-Columbia Basin region which encompasses the Bonneville Power Administration (BPA) wind generation area shown in Figure 1 that includes Klondike, Stateline, and Hopkins Ridge wind plants. The typical hub height of a wind turbine is approximately 80-m above ground level (AGL). So it would seem that building meteorological towers in the region upwind of a wind generation facility would provide data necessary to improve the short-term forecasts for the 80-m AGL wind speed. However, this additional meteorological information typically does not significantly improve the accuracy of the 0- to 6-hour ahead wind power forecasts because processes controlling wind variability change from day-to-day and, at times, from hour-to-hour. It is also important to note that some processes causing significant changes in wind power production function principally in the vertical direction. These processes will not be detected by meteorological towers at off-site locations. For these reasons, it is quite challenging to determine the best type of sensors and deployment locations. To address the measurement deployment problem, Ensemble Sensitivity Analysis (ESA) was applied in the Phase I portion of the WindSENSE project. The ESA approach was initially designed to produce spatial fields that depict the sensitivity of a forecast metric to a set of prior state variables selected by the user. The best combination of variables and locations to improve the forecast was determined using the Multiple Observation Optimization Algorithm (MOOA) developed in Phase I. In Zack et al. (2010a), the ESA-MOOA approach was

  7. Collateral projections of neurons in laminae I, III, and IV of rat spinal cord to thalamus, periaqueductal gray matter, and lateral parabrachial area.

    PubMed

    Al-Khater, Khulood M; Todd, Andrew J

    2009-08-20

    Projection neurons in lamina I, together with those in laminae III-IV that express the neurokinin 1 receptor (NK1r), form a major route through which nociceptive information reaches the brain. Axons of these cells innervate various targets, including thalamus, periaqueductal gray matter (PAG), and lateral parabrachial area (LPb), and many cells project to more than one target. The aims of this study were to quantify projections from cervical enlargement to PAG and LPb, to determine the proportion of spinothalamic neurons at lumbar and cervical levels that were labelled from PAG and LPb, and to investigate morphological differences between projection populations. The C7 segment contained fewer lamina I spinoparabrachial cells than L4, but a similar number of spino-PAG cells. Virtually all spinothalamic lamina I neurons at both levels were labelled from LPb and between one-third and one-half from PAG. This suggests that significant numbers project to all three targets. Spinothalamic lamina I neurons differed from those labelled only from LPb in that they were generally larger, were more often multipolar, and (in cervical enlargement) had stronger NK1r immunoreactivity. Most lamina III/IV NK1r cells at both levels projected to LPb, but few were labelled from PAG. The great majority of these cells in C7 and over one-fourth of those in L4 were spinothalamic, and at each level some projected to both thalamus and LPb. These results confirm that neurons in these laminae have extensive collateral projections and suggest that different neuronal subpopulations in lamina I have characteristic patterns of supraspinal projection.

  8. Nitric Oxide Synthase Type III Overexpression By Gene Therapy Exerts Antitumoral Activity In Mouse Hepatocellular Carcinoma.

    PubMed

    González, Raúl; De la Rosa, Ángel J; Romero-Brufau, Santiago; Barrera-Pulido, Lydia; Gallardo-Chamizo, Francisco; Pereira, Sheila; Marín, Luís M; Álamo, José M; Rodríguez-Hernández, Ángeles; Padillo, Francisco J; Muntané, Jordi

    2015-08-01

    Hepatocellular carcinoma develops in cirrhotic liver. The nitric oxide (NO) synthase type III (NOS-3) overexpression induces cell death in hepatoma cells. The study developed gene therapy designed to specifically overexpress NOS-3 in cultured hepatoma cells, and in tumors derived from orthotopically implanted tumor cells in fibrotic livers. Liver fibrosis was induced by CCl4 administration in mice. Hepa 1-6 cells were used for in vitro and in vivo experiments. The first generation adenovirus was designed to overexpress NOS-3 (or GFP) and luciferase cDNA under the regulation of murine alpha-fetoprotein (AFP) and Rous Sarcoma Virus (RSV) promoters, respectively. Both adenoviruses were administered through the tail vein two weeks after orthotopic tumor cell implantation. AFP-NOS-3/RSV-Luciferase increased oxidative-related DNA damage, p53, CD95/CD95L expression and caspase-8 activity in cultured Hepa 1-6 cells. The increased expression of CD95/CD95L and caspase-8 activity was abolished by l-NAME or p53 siRNA. The tail vein infusion of AFP-NOS- 3/RSV-Luciferase adenovirus increased cell death markers, and reduced cell proliferation of established tumors in fibrotic livers. The increase of oxidative/nitrosative stress induced by NOS-3 overexpression induced DNA damage, p53, CD95/CD95L expression and cell death in hepatocellular carcinoma cells. The effectiveness of the gene therapy has been demonstrated in vitro and in vivo.

  9. Remarkable in vitro bactericidal activity of bismuth(III) sulfonates against Helicobacter pylori.

    PubMed

    Andrews, Philip C; Busse, Madleen; Deacon, Glen B; Ferrero, Richard L; Junk, Peter C; MacLellan, Jonathan G; Vom, Amelia

    2012-10-14

    Four new tris-substituted bismuth(III) sulfonates of general formula [Bi(O(3)SR)(3)] (R = phenyl 1, p-tolyl 2, 2,4,6-mesityl 3 and S-(+)-10-camphoryl 4) have been synthesised and characterised. Their synthesis by solvent-free (SF) and solvent-mediated (SM) methods has been explored and their activity against Helicobacter pylori has been investigated. The compounds 1-4 display a remarkable in vitro activity against three laboratory strains of H. pylori (B128, 26,695 and 251) with minimum inhibitory concentration (MIC) values as low as 0.049 μg mL(-1) for the strains B128 and 26,695, and 0.781 μg mL(-1) for the clinical isolate 251. This places most MIC values in the nano-molar region and demonstrates the strong influence of the sulfonate group on the bactericidal properties. The novel solid state structure [Bi(8)(O(3)SMes)(20)(SO(4))(2)(H(2)O)(6)]·(C(7)H(8))(7)5·(C(7)H(8))(7), derived from the SM reaction under reflux conditions, is presented and the incorporation of the two inorganic sulfate anions in the centre of the wheel-like bismuth sulfonate cluster explained.

  10. Global Art: Activities, Projects, and Inventions from Around the World.

    ERIC Educational Resources Information Center

    Kohl, MaryAnn F.; Potter, Jean

    This activity book contains over 130 art ideas from around the world, combining the fun and creativity of art with the mysteries of history, the lure of geography, and the diversity of the cultures of the world. These projects allow children to explore the world through art with a process, not a product, approach to artistic outcome; the process…

  11. Mapping International Cancer Activities – Global Cancer Project Map Launch

    Cancer.gov

    CGH’s Dr. Sudha Sivaram, Dr. Makeda Williams, and Ms. Kalina Duncan have partnered with Drs. Ami Bhatt and Franklin Huang at Global Oncology, Inc. (GO) to develop the Global Cancer Project Map - a web-based tool designed to facilitate cancer research and control activity planning.

  12. "Our Leaders Are Us": Youth Activism in Social Movements Project

    ERIC Educational Resources Information Center

    Marri, Anand R.; Walker, Erica N.

    2008-01-01

    In this article, we describe the development and implementation of a project, "Our Leaders Are Us: Youth Activism in Social Movements," that we undertook with New York City high school students exploring events leading up to and following the Supreme Court's landmark decision in Brown. As part of a 50th anniversary celebration of the…

  13. Critters: K-6 Life Science Activities. Project AIMS.

    ERIC Educational Resources Information Center

    Allen, Maureen Murphy; And Others

    Project AIMS (Activities to Integrate Mathematics and Science) has as its purpose the integration of subject matter in grades K-9. Field testing of the curriculum materials produced by AIMS indicates that this integration produces the following beneficial results: (1) mathematics becomes more meaningful, hence more useful; (2) science is…

  14. Stratospheric Aerosol and Gas Experiment, SAGE III on ISS, An Earth Science Mission on the International Space Station, Schedule Risk Analysis, A Project Perspective

    NASA Technical Reports Server (NTRS)

    Bonine, Lauren

    2015-01-01

    The presentation provides insight into the schedule risk analysis process used by the Stratospheric Aerosol and Gas Experiment III on the International Space Station Project. The presentation focuses on the schedule risk analysis process highlighting the methods for identification of risk inputs, the inclusion of generic risks identified outside the traditional continuous risk management process, and the development of tailored analysis products used to improve risk informed decision making.

  15. Synthesis of activated carbon-based amino phosphonic acid chelating resin and its adsorption properties for Ce(III) removal.

    PubMed

    Chen, Tao; Yan, Chunjie; Wang, Yixia; Tang, Conghai; Zhou, Sen; Zhao, Yuan; Ma, Rui; Duan, Ping

    2015-01-01

    This work aims to investigate the adsorption of Ce(III) onto chelating resin based on activated carbon (CRAC). The CRAC adsorbent was prepared from activated carbon (AC) followed by oxidation, silane coupling, ammoniation and phosphorylation, and characterized by Fourier transform-infrared spectrometry, nitrogen adsorption measurements and scanning electron microscopy. The effects of solution pH, adsorbent dosage and contact time were studied by batch technique. Langmuir and Freundlich isotherms were used to describe the adsorption behaviour of Ce(III) by CRAC, and the results showed that the adsorption behaviour well fitted the Langmuir model. The maximum uptake capacity (qmax) calculated by using the Langmuir equation for cerium ions was found to be 94.34 mg/g. A comparison of the kinetic models and the overall experimental data was best fitted with the type 1 pseudo second-order kinetic model. The calculated thermodynamic parameters (ΔG°, ΔH° and ΔS°) showed that the adsorption for Ce(III) was feasible, spontaneous and exothermic at 25-45 °C. The CRAC showed an excellent adsorptive selectivity towards Ce(III). Moreover, more than 82% of Ce(III) adsorbed onto CRAC could be desorbed with HCl and could be used several times.

  16. ACTIVE IMMUNIZATION OF MICE WITH THE POLYSACCHARIDES OF PNEUMOCOCCI TYPES I, II AND III

    PubMed Central

    Zozaya, José; Clark, Janet

    1933-01-01

    1. Pneumococcus polysaccharides Types I, II and III adsorbed on collodion particles, and Types I and III adsorbed on carbon (norit) are antigenic in mice. 2. Unadsorbed pneumococcus polysaccharide of Type I is antigenic in mice in proper dilution. One preparation of Type II polysaccharide was not antigenic, while another one immunized against Types I and II. Type III polysaccharide was only slightly antigenic against Type III but immunized against Type I. 3. The antigenicity of pneumococcus polysaccharide in optimal dosage is tentatively explained by an adsorption phenomenon taking place in the body in instances in which the polysaccharides had not been adsorbed before injection. 4. The aggressin-like action of large doses of pneumococcus polysaccharides Types I, II and III is further established. PMID:19870119

  17. Transcriptional activation of Xenopus class III genes in chromatin isolated from sperm and somatic nuclei.

    PubMed Central

    Wolffe, A P

    1989-01-01

    Xenopus sperm chromatin lacks class III transcription complexes and somatic histone H1. Inactive class III genes in sperm chromatin are easily programmed with transcription complexes de novo and transcribed in Xenopus oocyte nuclear extract. In contrast, repressed class III genes in somatic chromatin are not transcribed in the oocyte nuclear extract. Class III genes that are initially inactive or repressed in both types of chromatin can be efficiently transcribed in a cell free preparation of Xenopus eggs. Chromatin mediated repression of class III genes in somatic nuclei is reversible in Xenopus egg extract, but not in the oocyte nuclear extract. Any inhibition of transcription attributed to chromatin assembly onto a gene, will therefore depend on the extract in which transcription is assayed. Images PMID:2915929

  18. Project Choices: Update on the Effectiveness of Adventure Activities Used for Treating Addictions.

    ERIC Educational Resources Information Center

    Gillis, H. L.

    This paper describes an adventure-based treatment program for court-involved youth who display significant alcohol or drug abuse behavior. Project Choices' clients meet the criteria for drug abuse from the Diagnostic Statistical Manual-Revised (DSM-III-R). The clients reside in Project Choices placement homes during the 8-week treatment and 8-week…

  19. 34 CFR 426.4 - What activities does the Secretary fund under the Demonstration Projects?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Secretary supports the following types of projects: (a) Model projects providing improved access to quality... instruction provided by an eligible recipient; (iii) Placement services in occupations that the students are... institutional and on-the-job training, supportive services authorized by the Act, and other assistance as...

  20. 34 CFR 426.4 - What activities does the Secretary fund under the Demonstration Projects?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Secretary supports the following types of projects: (a) Model projects providing improved access to quality... instruction provided by an eligible recipient; (iii) Placement services in occupations that the students are... institutional and on-the-job training, supportive services authorized by the Act, and other assistance as...

  1. Mutation of Arg-115 of human class III alcohol dehydrogenase: a binding site required for formaldehyde dehydrogenase activity and fatty acid activation.

    PubMed Central

    Engeland, K; Höög, J O; Holmquist, B; Estonius, M; Jörnvall, H; Vallee, B L

    1993-01-01

    The origin of the fatty acid activation and formaldehyde dehydrogenase activity that distinguishes human class III alcohol dehydrogenase (alcohol:NAD+ oxidoreductase, EC 1.1.1.1) from all other alcohol dehydrogenases has been examined by site-directed mutagenesis of its Arg-115 residue. The Ala- and Asp-115 mutant proteins were expressed in Escherichia coli and purified by affinity chromatography and ion-exchange HPLC. The activities of the recombinant native and mutant enzymes toward ethanol are essentially identical, but mutagenesis greatly decreases the kcat/Km values for glutathione-dependent formaldehyde oxidation. The catalytic efficiency for the Asp variant is < 0.1% that of the unmutated enzyme, due to both a higher Km and a lower kcat value. As with the native enzyme, neither mutant can oxidize methanol, be saturated by ethanol, or be inhibited by 4-methylpyrazole; i.e., they retain these class III characteristics. In contrast, however, their activation by fatty acids, another characteristic unique to class III alcohol dehydrogenase, is markedly attenuated. The Ala mutant is activated only slightly, but the Asp mutant is not activated at all. The results strongly indicate that Arg-115 in class III alcohol dehydrogenase is a component of the binding site for activating fatty acids and is critical for the binding of S-hydroxymethylglutathione in glutathione-dependent formaldehyde dehydrogenase activity. PMID:8460164

  2. Template synthesis, spectroscopic studies, antimicrobial, nematicidal and pesticidal activities of chromium(III) macrocyclic complexes.

    PubMed

    Masih, Iffat; Fahmi, Nighat; Rajkumar

    2013-02-01

    A new series of Cr(III) macrocyclic complexes have been synthesized by template condensation of ligands 2-[4-chloro-2-(2-oxo-1,2-diphenyl-ethylideneamino)-phenylimino]-1,2-diphenyl-ethanone (ML(1)) and 2-[4-fluro-2-(2-oxo-1,2-diphenyl-ethylideneamino)-phenylimino]-1,2-diphenyl-ethanone (ML(2)) respectively, with appropriate diamines i.e. 1,2-phenylenediamine, 4- chloro 1,2-phenylenediamine and 4-fluro- 1,2-phenylenediamine in the presence of CrCl(3).6H(2)O. The ligands and their complexes have been characterized on the basis of elemental analyses, molecular weight determinations, conductance and magnetic susceptibility measurements and spectral studies including IR, ESR, electronic spectra and X-ray powder diffraction studies. On the basis of these studies, a six-coordinated octahedral geometry has been proposed for all these complexes. The newly synthesized ligands and their complexes have been screened for their antimicrobial, nematicidal and pesticidal activities. The results are indeed positive.

  3. Myosin III-mediated cross-linking and stimulation of actin bundling activity of Espin

    PubMed Central

    Liu, Haiyang; Li, Jianchao; Raval, Manmeet H; Yao, Ningning; Deng, Xiaoying; Lu, Qing; Nie, Si; Feng, Wei; Wan, Jun; Yengo, Christopher M; Liu, Wei; Zhang, Mingjie

    2016-01-01

    Class III myosins (Myo3) and actin-bundling protein Espin play critical roles in regulating the development and maintenance of stereocilia in vertebrate hair cells, and their defects cause hereditary hearing impairments. Myo3 interacts with Espin1 through its tail homology I motif (THDI), however it is not clear how Myo3 specifically acts through Espin1 to regulate the actin bundle assembly and stabilization. Here we discover that Myo3 THDI contains a pair of repeat sequences capable of independently and strongly binding to the ankyrin repeats of Espin1, revealing an unexpected Myo3-mediated cross-linking mechanism of Espin1. The structures of Myo3 in complex with Espin1 not only elucidate the mechanism of the binding, but also reveal a Myo3-induced release of Espin1 auto-inhibition mechanism. We also provide evidence that Myo3-mediated cross-linking can further promote actin fiber bundling activity of Espin1. DOI: http://dx.doi.org/10.7554/eLife.12856.001 PMID:26785147

  4. Rhodium(III)-Catalyzed Enantiotopic C-H Activation Enables Access to P-Chiral Cyclic Phosphinamides.

    PubMed

    Sun, Yang; Cramer, Nicolai

    2017-01-02

    Compounds with stereogenic phosphorus atoms are frequently used as ligands for transition-metal as well as organocatalysts. A direct catalytic enantioselective method for the synthesis of P-chiral compounds from easily accessible diaryl phosphinamides is presented. The use of rhodium(III) complexes equipped with a suitable atropochiral cyclopentadienyl ligand is shown to enable an enantiodetermining C-H activation step. Upon trapping with alkynes, a broad variety of cyclic phosphinamides with a stereogenic phosphorus(V) atom are formed in high yields and enantioselectivities. Moreover, these can be reduced enantiospecifically to P-chiral phosphorus(III) compounds.

  5. Idaho Water Rental Pilot Project Probability/Coordination Study Resident Fish and Wildlife Impacts Phase III, 1997 Annual Report.

    SciTech Connect

    Leitzinger, Eric J.

    1998-10-01

    Phase III began in 1995 with the overall goal of quantifying changes in resident fish habitat in the Snake River Basin upstream of Brownlee Reservoir resulting from the release of salmon flow augmentation water.

  6. Regulation of yeast ESCRT-III membrane scission activity by the Doa4 ubiquitin hydrolase.

    PubMed

    Johnson, Natalie; West, Matt; Odorizzi, Greg

    2017-03-01

    ESCRT-III executes membrane scission during the budding of intralumenal vesicles (ILVs) at endosomes. The scission mechanism is unknown but appears to be linked to the cycle of assembly and disassembly of ESCRT-III complexes at membranes. Regulating this cycle is therefore expected to be important for determining the timing of ESCRT-III-mediated membrane scission. We show that in Saccharomyces cerevisiae, ESCRT-III complexes are stabilized and ILV membrane scission is delayed by Doa4, which is the ubiquitin hydrolase that deubiquitinates transmembrane proteins sorted as cargoes into ILVs. These results suggest a mechanism to delay ILV budding while cargoes undergo deubiquitination. We further show that deubiquitination of ILV cargoes is inhibited via Doa4 binding to Vps20, which is the subunit of ESCRT-III that initiates assembly of the complex. Current models suggest that ESCRT-III complexes surround ubiquitinated cargoes to trap them at the site of ILV budding while the cargoes undergo deubiquitination. Thus our results also propose a mechanism to prevent the onset of ILV cargo deubiquitination at the initiation of ESCRT-III complex assembly.

  7. TATA-box DNA binding activity and subunit composition for RNA polymerase III transcription factor IIIB from Xenopus laevis.

    PubMed Central

    McBryant, S J; Meier, E; Leresche, A; Sharp, S J; Wolf, V J; Gottesfeld, J M

    1996-01-01

    The RNA polymerase III transcription initiation factor TFIIIB contains the TATA-box-binding protein (TBP) and polymerase III-specific TBP-associated factors (TAFs). Previous studies have shown that DNA oligonucleotides containing the consensus TATA-box sequence inhibit polymerase III transcription, implying that the DNA binding domain of TBP is exposed in TFIIIB. We have investigated the TATA-box DNA binding activity of Xenopus TFIIIB, using transcription inhibition assays and a gel mobility shift assay. Gel shift competition assays with mutant and nonspecific DNAs demonstrate the specificity of the TFIIIB-TATA box DNA complex. The apparent dissociation constant for this protein-DNA interaction is approximately 0.4 nM, similar to the affinity of yeast TBP for the same sequence. TFIIIB transcriptional activity and TATA-box binding activity cofractionate during a series of four ion-exchange chromatographic steps, and reconstituted transcription reactions demonstrate that the TATA-box DNA-protein complex contains TFIIIB TAF activity. Polypeptides with apparent molecular masses of 75 and 92 kDa are associated with TBP in this complex. These polypeptides were renatured after elution from sodium dodecyl sulfate-gels and tested individually and in combination for TFIIIB TAF activity. Recombinant TBP along with protein fractions containing the 75- and 92-kDa polypeptides were sufficient to reconstitute TFIIIB transcriptional activity and DNA binding activity, suggesting that Xenopus TFIIIB is composed of TBP along with these polypeptides. PMID:8756620

  8. Title III and Cultural Diversity.

    ERIC Educational Resources Information Center

    The Title III Quarterly, 1973

    1973-01-01

    Title III projects dealing with cultural diversity in the classroom are described in this issue of the Title III Quarterly. Major articles are devoted to the following projects: Two Arts Culture Three Project, developing the crafts and music of mountain whites, blacks, and Cherokees; the Rota Bilingual Project, the Marianas District, emphasizing…

  9. Influence of chirality using Mn(III) salen complexes on DNA binding and antioxidant activity.

    PubMed

    Khan, Noor-Ul H; Pandya, Nirali; Kumar, Manoj; Bera, Prasanta Kumar; Kureshy, Rukhsana I; Abdi, Sayed H R; Bajaj, Hari C

    2010-10-07

    Chiral Mn(iii) salen complexes S-1, R-1, S-2, R-2, S-3 and R-3 derived from the respective chiral salen ligands, viz., (1S,2S)-N,N'-bis-[3-tert-butyl-5-chloromethyl-salicylidine]-1,2-cyclohexanediamine S-1'/(1R,2R)-N,N'-bis-[3-tert-butyl-5-chloromethyl-salicylidine]-1,2-cyclohexanediamine R-1'/(1S,2S)-N,N'-bis-[3-tert-butyl-5-N,N'N'triethylaminomethyl-salicylidine]-1,2-cyclohexanediamine dichloride S-2'/(1R,2R)-N,N'-bis-[3-tert-butyl-5-N,N'N'triethylaminomethyl-salicylidine]-1,2-cyclohexanediamine dichloride R-2'/(1S,2S)-N,N'-bis-[3,5-di-tert-butylsalicylidene]-1,2-cyclohexanediamine S-3' and (1R,2R)-N,N'-bis-[3,5-di-tert-butyl-salicylidene]-1,2-cyclohexanediamine R-3', were synthesized. Characterization of the complexes was done by microanalysis, IR, LC-MS, UV-vis. and circular dichroism (CD) spectroscopy. Binding of these complexes with calf thymus DNA (CT-DNA) was studied by absorption spectroscopy, competitive binding study, viscosity measurements, circular dichroism measurements, thermal denaturation study and observation of their different antioxidant activities. Among all the complexes used, the best result in terms of binding constant (intercalative) (130.4 x 10(4)) was achieved with the complex S-1 by spectroscopic titration. The complex S-1 showed strong antioxidant activity as well.

  10. Fluorescence studies, DNA binding properties and antimicrobial activity of a dysprosium(III) complex containing 1,10-phenanthroline.

    PubMed

    Khorasani-Motlagh, Mozhgan; Noroozifar, Meissam; Moodi, Asieh; Niroomand, Sona

    2013-10-05

    Luminescence and binding properties of dysprosium(III) complex containing 1,10-phenanthroline (phen), [Dy(phen)2(OH2)3Cl]Cl2⋅H2O with DNA has been studied by electronic absorption, emission spectroscopy and viscosity measurement. The thermodynamic studies suggest that the interaction process to be endothermic and entropically driven, which indicates that the dysprosium(III) complex might interact with DNA by a non intercalation binding mode. Additionally, the competitive fluorescence study with ethidium bromide and also the effect of iodide ion and salt concentration on fluorescence of the complex-DNA system is investigated. Experimental results indicate that the Dy(III) complex strongly binds to DNA, presumably via groove binding mode. Furthermore, the complex shows a potent antibacterial activity and DNA cleavage ability.

  11. Synthesis and characterization of chromium(III) Schiff base complexes: antimicrobial activity and its electrocatalytic sensing ability of catechol.

    PubMed

    Kumar, S Praveen; Suresh, R; Giribabu, K; Manigandan, R; Munusamy, S; Muthamizh, S; Narayanan, V

    2015-03-15

    A series of acyclic Schiff base chromium(III) complexes were synthesized with the aid of microwave irradiation method. The complexes were characterized on the basis of elemental analysis, spectral analysis such as UV-Visible, Fourier transform infrared (FT-IR), nuclear magnetic resonance (NMR), electron paramagnetic resonance (EPR) spectroscopies and electrospray ionization (ESI) mass spectrometry. Electrochemical analysis of the complexes indicates the presence of chromium ion in +3 oxidation state. Cr (III) ion is stabilized by the tetradentate Schiff base ligand through its nitrogen and phenolic oxygen. From the spectral studies it is understood that the synthesized chromium(III) complexes exhibits octahedral geometry. Antimicrobial activity of chromium complexes was investigated towards the Gram positive and Gram negative bacteria. In the present work, an attempt was made to fabricate a new kind of modified electrode based on chromium Schiff base complexes for the detection of catechol at nanomolar level.

  12. Synthesis and characterization of chromium(III) Schiff base complexes: Antimicrobial activity and its electrocatalytic sensing ability of catechol

    NASA Astrophysics Data System (ADS)

    Praveen Kumar, S.; Suresh, R.; Giribabu, K.; Manigandan, R.; Munusamy, S.; Muthamizh, S.; Narayanan, V.

    2015-03-01

    A series of acyclic Schiff base chromium(III) complexes were synthesized with the aid of microwave irradiation method. The complexes were characterized on the basis of elemental analysis, spectral analysis such as UV-Visible, Fourier transform infrared (FT-IR), nuclear magnetic resonance (NMR), electron paramagnetic resonance (EPR) spectroscopies and electrospray ionization (ESI) mass spectrometry. Electrochemical analysis of the complexes indicates the presence of chromium ion in +3 oxidation state. Cr (III) ion is stabilized by the tetradentate Schiff base ligand through its nitrogen and phenolic oxygen. From the spectral studies it is understood that the synthesized chromium(III) complexes exhibits octahedral geometry. Antimicrobial activity of chromium complexes was investigated towards the Gram positive and Gram negative bacteria. In the present work, an attempt was made to fabricate a new kind of modified electrode based on chromium Schiff base complexes for the detection of catechol at nanomolar level.

  13. Polymer-cobalt(III) complexes: structural analysis of metal chelates on DNA interaction and comparative cytotoxic activity.

    PubMed

    Nehru, Selvan; Arunachalam, Sankaralingam; Arun, Renganathan; Premkumar, Kumpati

    2014-01-01

    A new series of pendant-type polymer-cobalt(III) complexes, [Co(LL)2(BPEI)Cl](2+), (where BPEI = branched polyethyleneimine, LL = dipyrido[3,2-a:2',3'-c](6,7,8,9-tetrahydro)phenazine (dpqc), dipyrido[3,2-d:2',3'-f]quinoxaline (dpq) and imidazo[4,5-f]1,10-phenanthroline (ip)) each with three different degrees of coordination have been synthesized and characterized. Studies to know the mode and strength of interaction between these polymer-metal complexes and calf thymus DNA have been performed by UV-Visible absorption and emission techniques. Among these series, each polymer metal complex having higher binding strength with DNA has been selected to test against human cancer/normal cell lines. On the basis of these spectral studies, it is proposed that our polymer-metal complexes bind with DNA mainly through intercalation along with some electrostatic binding. The order of binding strength for the complexes with ligand, dpqc > dpq > ip. The analysis of the results suggests that polymer-cobalt(III) complexes with higher degree of coordination effectively binds with DNA due to the presence of large number of positively charged cobalt(III) chelates in the polymer chain which cooperatively act to increase the overall binding strength. These polymer-cobalt(III) complexes with hydrophobic ligands around the cobalt(III) metal centre favour the base stacking interactions via intercalation. All the complexes show very good anticancer activities and increasing of binding strength results in higher inhibition value. The polymer-cobalt(III) complex with dpqc ligand possess two fold increased anticancer activity when compared to complexes with other ligands against MCF-7 cells. Besides, the complexes were insensitive towards the growth of normal cells (HEK-293) at the IC50 concentration.

  14. Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6

    PubMed Central

    Niewoehner, Ole; Jinek, Martin

    2016-01-01

    Prokaryotic CRISPR–Cas systems provide an RNA-guided mechanism for genome defense against mobile genetic elements such as viruses and plasmids. In type III-A CRISPR–Cas systems, the RNA-guided multisubunit Csm effector complex targets both single-stranded RNAs and double-stranded DNAs. In addition to the Csm complex, efficient anti-plasmid immunity mediated by type III-A systems also requires the CRISPR-associated protein Csm6. Here we report the crystal structure of Csm6 from Thermus thermophilus and show that the protein is a ssRNA-specific endoribonuclease. The structure reveals a dimeric architecture generated by interactions involving the N-terminal CARF and C-terminal HEPN domains. HEPN domain dimerization leads to the formation of a composite ribonuclease active site. Consistently, mutations of invariant active site residues impair catalytic activity in vitro. We further show that the ribonuclease activity of Csm6 is conserved across orthologs, suggesting that it plays an important functional role in CRISPR–Cas systems. The dimer interface of the CARF domains features a conserved electropositive pocket that may function as a ligand-binding site for allosteric control of ribonuclease activity. Altogether, our work suggests that Csm6 proteins provide an auxiliary RNA-targeting interference mechanism in type III-A CRISPR–Cas systems that operates in conjunction with the RNA- and DNA-targeting endonuclease activities of the Csm effector complex. PMID:26763118

  15. Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6.

    PubMed

    Niewoehner, Ole; Jinek, Martin

    2016-03-01

    Prokaryotic CRISPR-Cas systems provide an RNA-guided mechanism for genome defense against mobile genetic elements such as viruses and plasmids. In type III-A CRISPR-Cas systems, the RNA-guided multisubunit Csm effector complex targets both single-stranded RNAs and double-stranded DNAs. In addition to the Csm complex, efficient anti-plasmid immunity mediated by type III-A systems also requires the CRISPR-associated protein Csm6. Here we report the crystal structure of Csm6 from Thermus thermophilus and show that the protein is a ssRNA-specific endoribonuclease. The structure reveals a dimeric architecture generated by interactions involving the N-terminal CARF and C-terminal HEPN domains. HEPN domain dimerization leads to the formation of a composite ribonuclease active site. Consistently, mutations of invariant active site residues impair catalytic activity in vitro. We further show that the ribonuclease activity of Csm6 is conserved across orthologs, suggesting that it plays an important functional role in CRISPR-Cas systems. The dimer interface of the CARF domains features a conserved electropositive pocket that may function as a ligand-binding site for allosteric control of ribonuclease activity. Altogether, our work suggests that Csm6 proteins provide an auxiliary RNA-targeting interference mechanism in type III-A CRISPR-Cas systems that operates in conjunction with the RNA- and DNA-targeting endonuclease activities of the Csm effector complex.

  16. The Haida Gwaii Diabetes Project: planned response activity outcomes.

    PubMed

    Heffernan, Clare; Herbert, Carol; Grams, Garry D.; Grzybowski, Stefan; Wilson, Mary Ann; Calam, Betty; Brown, Diane

    1999-11-01

    A 1992 chart review in the Haida Village of Skidegate, Haida Gwaii/Queen Charlotte Islands, Canada, revealed that 17% of the unscreened population aged 35 or over have been diagnosed with diabetes. The Haida Gwaii Diabetes Project was designed to develop a culturally sensitive community-based participatory action approach to the management of noninsulin-dependent diabetes (NIDDM). Phase One included obtaining community support, conducting a chart review, holding clinics to measure biophysical indicators, conducting focus groups, and planning response activities with the communities. A list of activities was developed, based on the focus group results. In addition, the project team developed a set of operating principles that ensured and reinforced collaboration. Phase Two included implementing and monitoring planned response activities, and holding exit clinics. The best attended activities were trials of traditional herbal medicine and traditional diet, and an exercise programme. While participation levels were not high enough for causal conclusions, a significant decrease in total cholesterol (0.45; P = 0.005) and rise in HDL (-0.097; P = 0.05) was found for participants for whom paired values were available. Diabetes intervention research in First Nations settings involves small numbers of participants, making it difficult to quantitatively assess outcomes. To increase participation it is advisable to open activities to the whole community, to tie planned activities into other scheduled community events, and to share findings concerning managing illnesses of acculturation with other communities.

  17. The fenton activity of iron(III) in the presence of deferiprone.

    PubMed

    Devanur, Lakshmi D; Neubert, Hendrik; Hider, Robert C

    2008-04-01

    Hydroxyl radical production from a range of clinically relevant iron chelators in the presence of hydrogen peroxide was measured using the deoxyribose oxidation assay. Hydroxyl radical production from an iron complex is dependent on whether the ligand is able to completely surround the iron, thereby preventing access of reductants to the coordinated iron cation. The partially coordinated [(deferiprone)(2)Fe(III)](+) complex is able to generate hydroxyl radicals in the presence of oxidants, whereas the fully coordinated [(deferiprone)(3)Fe(III)](0) complex is not. Hydroxyl radical production from iron(III)deferiprone complexes is dependent on the molar ratio of iron to deferiprone, which, in turn, affects the speciation of the complex. Mass spectrometry data have confirmed the presence of the [(deferiprone)(2)Fe(III)](+) complex in aqueous solution. Hydroxyl radical production from the [(deferiprone)(2)Fe(III)](+) complex is maximal in the presence of equimolar ascorbate and hydrogen peroxide and is abolished in the absence of hydrogen peroxide. Under biological conditions, any [(deferiprone)(2)Fe(III)](+) complex formed intracellularly will be rapidly reduced by ascorbate. The resulting unstable iron(II) complex will dissociate to hexa-aquo iron(II), a major component of the endogenous intracellular labile iron pool.

  18. Receptor dimerization is not a factor in the signalling activity of a transforming variant epidermal growth factor receptor (EGFRvIII).

    PubMed Central

    Chu, C T; Everiss, K D; Wikstrand, C J; Batra, S K; Kung, H J; Bigner, D D

    1997-01-01

    The type-III deletion variant of the epidermal growth factor receptor (EGFRvIII) is frequently found in glioblastomas and other malignant human tumours. Although EGFRvIII confers ligand-independent oncogenic transformation of cell lines, the mechanism by which it promotes aberrant cellular proliferation is unknown. Using cell lines expressing comparable numbers of either wild-type receptor (EGFRwt) or EGFRvIII, we compared several parameters of receptor activation: dimerization, tyrosine phosphorylation and activation of intracellular signalling proteins. Like activated EGFRwt, EGFRvIII was phosphorylated and bound constitutively to the Shc adapter protein. Indeed, EGFRvIII-associated Shc had a higher phosphotyrosine content than Shc associated with stimulated EGFRwt. EGFRwt dimerized in response to either EGF or transforming growth factor alpha. Higher cross-linker concentrations and incubation at higher temperatures (37 degrees C) allowed detection of EGFRwt dimers even in the absence of exogenous ligand. In contrast, EGFRvIII failed to dimerize under any conditions studied. Moreover, neither mitogen-activated protein kinase nor phospholipase Cgamma were phosphorylated in EGFRvIII-expressing cells. We conclude that the deletion of 267 amino acids from the 621-amino-acid N-terminal domain of EGFR does not result simply in a constitutively activated receptor, but alters the spectrum of signalling cascades utilized. Furthermore the ligand-independent transforming activity of EGFRvIII is independent of receptor dimerization. PMID:9210410

  19. Projecting climate-driven increases in North American fire activity

    NASA Astrophysics Data System (ADS)

    Wang, D.; Morton, D. C.; Collatz, G. J.

    2013-12-01

    Climate regulates fire activity through controls on vegetation productivity (fuels), lightning ignitions, and conditions governing fire spread. In many regions of the world, human management also influences the timing, duration, and extent of fire activity. These coupled interactions between human and natural systems make fire a complex component of the Earth system. Satellite data provide valuable information on the spatial and temporal dynamics of recent fire activity, as active fires, burned area, and land cover information can be combined to separate wildfires from intentional burning for agriculture and forestry. Here, we combined satellite-derived burned area data with land cover and climate data to assess fire-climate relationships in North America between 2000-2012. We used the latest versions of the Global Fire Emissions Database (GFED) burned area product and Modern-Era Retrospective Analysis for Research and Applications (MERRA) climate data to develop regional relationships between burned area and potential evaporation (PE), an integrated dryness metric. Logistic regression models were developed to link burned area with PE and individual climate variables during and preceding the fire season, and optimal models were selected based on Akaike Information Criterion (AIC). Overall, our model explained 85% of the variance in burned area since 2000 across North America. Fire-climate relationships from the era of satellite observations provide a blueprint for potential changes in fire activity under scenarios of climate change. We used that blueprint to evaluate potential changes in fire activity over the next 50 years based on twenty models from the Coupled Model Intercomparison Project Phase 5 (CMIP5). All models suggest an increase of PE under low and high emissions scenarios (Representative Concentration Pathways (RCP) 4.5 and 8.5, respectively), with largest increases in projected burned area across the western US and central Canada. Overall, near

  20. Biochemical investigation of yttrium(III) complex containing 1,10-phenanthroline: DNA binding and antibacterial activity.

    PubMed

    Khorasani-Motlagh, Mozhgan; Noroozifar, Meissam; Moodi, Asieh; Niroomand, Sona

    2013-03-05

    Characterization of the interaction between yttrium(III) complex containing 1,10-phenanthroline as ligand, [Y(phen)2Cl(OH2)3]Cl2⋅H2O, and DNA has been carried out by UV absorption, fluorescence spectra and viscosity measurements in order to investigate binding mode. The experimental results indicate that the yttrium(III) complex binds to DNA and absorption is decreasing in charge transfer band with the increase in amount of DNA. The binding constant (Kb) at different temperatures as well as thermodynamic parameters, enthalpy change (ΔH°) and entropy change (ΔS°), were calculated according to relevant fluorescent data and Vant' Hoff equation. The results of interaction mechanism studies, suggested that groove binding plays a major role in the binding of the complex and DNA. The activity of yttrium(III) complex against some bacteria was tested and antimicrobial screening tests shown growth inhibitory activity in the presence of yttrium(III) complex.

  1. Characterization and Properties of Activated Carbon Prepared from Tamarind Seeds by KOH Activation for Fe(III) Adsorption from Aqueous Solution

    PubMed Central

    Mopoung, Sumrit; Moonsri, Phansiri; Palas, Wanwimon; Khumpai, Sataporn

    2015-01-01

    This research studies the characterization of activated carbon from tamarind seed with KOH activation. The effects of 0.5 : 1–1.5 : 1 KOH : tamarind seed charcoal ratios and 500–700°C activation temperatures were studied. FTIR, SEM-EDS, XRD, and BET were used to characterize tamarind seed and the activated carbon prepared from them. Proximate analysis, percent yield, iodine number, methylene blue number, and preliminary test of Fe(III) adsorption were also studied. Fe(III) adsorption was carried out by 30 mL column with 5–20 ppm Fe(III) initial concentrations. The percent yield of activated carbon prepared from tamarind seed with KOH activation decreased with increasing activation temperature and impregnation ratios, which were in the range from 54.09 to 82.03 wt%. The surface functional groups of activated carbon are O–H, C=O, C–O, –CO3, C–H, and Si–H. The XRD result showed high crystallinity coming from a potassium compound in the activated carbon. The main elements found in the activated carbon by EDS are C, O, Si, and K. The results of iodine and methylene blue adsorption indicate that the pore size of the activated carbon is mostly in the range of mesopore and macropore. The average BET pore size and BET surface area of activated carbon are 67.9764 Å and 2.7167 m2/g, respectively. Finally, the tamarind seed based activated carbon produced with 500°C activation temperature and 1.0 : 1 KOH : tamarind seed charcoal ratio was used for Fe(III) adsorption test. It was shown that Fe(III) was adsorbed in alkaline conditions and adsorption increased with increasing Fe(III) initial concentration from 5 to 20 ppm with capacity adsorption of 0.0069–0.019 mg/g. PMID:26689357

  2. Cytotoxic activity of new cerium (III) complexes of bis-coumarins.

    PubMed

    Kostova, Irena; Manolov, Ilia; Momekov, Georgi; Tzanova, Tzvetomira; Konstantinov, Spiro; Karaivanova, Margarita

    2005-12-01

    Complexes of cerium (III) with bis-coumarins: 3,3'-benzylidene-bis(4-hydroxy-2H-1-benzopyran-2-one) and bis(4-hydroxy-2-oxo-2H-chromen-3-yl)-(1H-pyrazol-3-yl)-methane were synthesized by reaction of cerium (III) salt and the ligands, in amounts equal to metal/ligand molar ratio of 1:2. The complexes were prepared by adding an aqueous solution of cerium (III) salt to an aqueous solution of the ligand subsequently raising the pH of the mixture gradually to ca. 5.0 by adding dilute solution of sodium hydroxide. The cerium (III) complexes with bis-coumarins were characterized by different physicochemical methods--elemental analysis, IR-, 1H- and 13C-NMR-spectroscopies and mass-spectral data. The spectral data of cerium (III) complexes were interpreted on the basis of comparison with the spectra of the free ligands. This analysis showed that in the Ce (III) complexes the ligands coordinated to the metal ion through both deprotonated hydroxyl groups. On the basis of the nu(C=O) red shift observed, participation of the carbonyl groups in the coordination to the metal ion was also suggested. Cytotoxic screening by MTT assay was carried out. In the present study we performed comparative evaluation of the cytotoxic effects of the two newly synthesized cerium complexes against the acute myeloid leukemia derived HL-60 and the chronic myeloid leukemia (CML)-derived BV-173. In addition the cytotoxic effects of Ce (III) complex with 3,3'-benzylidene-bis(4-hydroxy-2H-1-benzopyran-2-one) were evaluated on the CML-derived K-562 and LAMA-84 cells, characterized by relative low responsiveness to chemotherapy. The DNA isolated from the cytosolic fraction of BV-173 cells after 24 h treatment with the same complex (at 100 and 200 microM) demonstrated a laddering phenomenon that is indicative for apoptotic cell death.

  3. The OPTX Project. V. Identifying Distant Active Galactic Nuclei

    NASA Astrophysics Data System (ADS)

    Trouille, L.; Barger, A. J.; Tremonti, C.

    2011-11-01

    The Baldwin, Phillips, and Terlevich emission-line ratio diagnostic ([O III]/Hβ versus [N II]/Hα, hereafter BPT diagram) efficiently separates galaxies whose signal is dominated by star formation (BPT-SF) from those dominated by active galactic nucleus (AGN) activity (BPT-AGN). Yet this BPT diagram is limited to z < 0.5, the redshift at which [N II]λ6584 leaves the optical spectral window. Using the Sloan Digital Sky Survey (SDSS), we construct a new diagnostic, or TBT diagram, that is based on rest-frame g - z color, [Ne III]λ3869, and [O II]λλ3726 + 3729 and can be used for galaxies out to z < 1.4. The TBT diagram identifies 98.7% of the SDSS BPT-AGN as TBT-AGN and 97% of the SDSS BPT-SF as TBT-SF. Furthermore, it identifies 97% of the OPTX Chandra X-ray-selected AGNs as TBT-AGN. This is in contrast to the BPT diagram, which misidentifies 20% of X-ray-selected AGNs as BPT-SF. We use the Great Observatories Origins Deep Survey North and Lockman Hole galaxy samples, with their accompanying deep Chandra imaging, to perform X-ray and infrared stacking analyses to further validate our TBT-AGN and TBT-SF selections; that is, we verify the dominance of AGN activity in the former and star formation activity in the latter. Finally, we address the inclusion of the majority of the BPT-comp (sources lying between the BPT-SF and BPT-AGN regimes) in our TBT-AGN regime. We find that the stacked BPT-comp source is X-ray hard (langΓeffrang = 1.0+0.4 -0.4) and has a high X-ray luminosity to total infrared luminosity ratio. This suggests that, on average, the X-ray signal in BPT-comp is dominated by obscured or low accretion rate AGN activity rather than by star formation, supporting their inclusion in the TBT-AGN regime. Some of the data presented herein were obtained at the W. M. Keck Observatory, which is operated as a scientific partnership among the California Institute of Technology, the University of California, and the National Aeronautics and Space Administration

  4. THE UNITED PRESBYTERIAN NATIONAL EDUCATIONAL SURVEY, AN INTERDISCIPLINARY RESEARCH PROJECT. VOLUME III, RESEARCH INSTRUMENTS AND TABULATIONS OF RESEARCH DATA.

    ERIC Educational Resources Information Center

    WHITMAN, LAURIS B.; AND OTHERS

    THE DEPARTMENT OF RESEARCH OF THE NATIONAL COUNCIL OF CHURCHES CONDUCTED A SURVEY FOR THE UNITED PRESBYTERIAN CHURCH OF ITS MEMBERSHIP AND RELIGIOUS BELIEFS. VOLUME III OF ITS REPORT IS THE STUDY APPENDIXES. THERE ARE MARGINAL TABULATIONS ON THE SAMPLE CHURCHES AND COMMUNITY BACKGROUND, WHICH INCLUDE SUCH INFORMATION AS MEMBERSHIP, BUDGET,…

  5. The Design and Implementation of a Model Evaluation Capability. 1975-76 Final Report. Title III Project.

    ERIC Educational Resources Information Center

    Austin Independent School District, TX. Office of Research and Evaluation.

    The Austin Independent School District received an Elementary and Secondary Education Act Title III grant in 1973 to develop an internal research and evaluation capability. Funding was provided the resulting Office of Research and Evaluation (ORE) for three years. The foci of the original grant were (1) to develop a district evaluation model, (2)…

  6. [NiIII(OMe)]-mediated reductive activation of CO2 affording a Ni(κ1-OCO) complex

    DOE PAGES

    Chiou, Tzung -Wen; Tseng, Yen -Ming; Lu, Tsai -Te; ...

    2016-02-24

    Here, carbon dioxide is expected to be employed as an inexpensive and potential feedstock of C1 sources for the mass production of valuable chemicals and fuel. Versatile chemical transformations of CO2, i.e. insertion of CO2 producing bicarbonate/acetate/formate, cleavage of CO2 yielding μ-CO/μ-oxo transition-metal complexes, and electrocatalytic reduction of CO2 affording CO/HCOOH/CH3OH/CH4/C2H4/oxalate were well documented. Herein, we report a novel pathway for the reductive activation of CO2 by the [NiIII(OMe)(P(C6H3-3-SiMe3-2-S)3)]– complex, yielding the [NiIII(κ1-OCO˙–)(P(C6H3-3-SiMe3-2-S)3)]– complex. The formation of this unusual NiIII(κ1-OCO˙–) complex was characterized by single-crystal X-ray diffraction, EPR, IR, SQUID, Ni/S K-edge X-ray absorption spectroscopy, and Ni valence-to-core X-ray emissionmore » spectroscopy. The inertness of the analogous complexes [NiIII(SPh)], [NiII(CO)], and [NiII(N2H4)] toward CO2, in contrast, demonstrates that the ionic [NiIII(OMe)] core attracts the binding of weak σ-donor CO2 and triggers the subsequent reduction of CO2 by the nucleophilic [OMe]– in the immediate vicinity. This metal–ligand cooperative activation of CO2 may open a novel pathway promoting the subsequent incorporation of CO2 in the buildup of functionalized products.« less

  7. Hyperglycemia-conditioned increase in alpha-2-macroglobulin in healthy normal subjects: a phenomenon correlated with deficient antithrombin III activity.

    PubMed

    Ceriello, A; Quatraro, A; Dello Russo, P; Marchi, E; Barbanti, M; Giugliano, D

    1989-01-01

    Induced hyperglycemia in normal subjects increases alpha 2-macroglobulin (alpha 2M) activity and alpha 2M concentration and reduces antithrombin III (ATIII) activity, while it does not affect ATIII plasma concentration. Hyperglycemia-determined variations in ATIII activity and alpha 2M molecules are correlated in an inverse and parallel fashion. A compensatory role for the increase in alpha 2M in the regulation of the coagulation system may be hypothesized. Moreover, these data provide evidence that hyperglycemia may decrease, directly, the biological function of some proteins and may influence the levels of some risk factors for the development of complications in diabetes.

  8. Mechanistic Insight into the Rh(III)-Catalyzed C-H Activation of 2-Acetyl-1-Arythydrazines in Water.

    PubMed

    Wu, Weirong; Liu, Tao; Huang, Caiyun; Zhang, Jing; Man, Xiaoping

    2017-03-02

    A mechanistic study of the Cp*Rh(III)-catalyzed C-H functionalization of 2-acetyl-1-arythydrazines with diazo compounds in water was carried out by using density functional theory calculations. The results reveal that the acetyl-bonded N-H deprotonation is prior to the phenyl C-H activation. The mechanisms from protonation by acetic acid disagree with the proposal by the Wang group. Different from the Rh(III)-catalyzed C-H activation reported by experimental literature, the rate-determining step of the whole catalytic cycle with an overall barrier of 31.7 kcal mol(-1) (IV → TS12-P') is the protonation process of hydroxy O rather than the C-H bond cleavage step. The present theoretical study rationalizes the experimental observation at the molecular level.

  9. A thermochromic europium(iii) room temperature ionic liquid with thermally activated anion-cation interactions.

    PubMed

    Monteiro, Bernardo; Outis, Mani; Cruz, Hugo; Leal, João Paulo; Laia, César A T; Pereira, Cláudia C L

    2017-01-10

    We report the first example of an observable and reversible case of thermochromism due to the interaction of an alkylphosphonium (P6,6,6,14)(+) with a β-diketonate (1,1,1,2,2,3,3-heptafluoro-7,7-dimethyloctane-4,6-dionate-fod) of an europium(iii) tetrakis-β-diketonate room temperature ionic liquid. This thermochromism is characterized by the conversion of a light yellow viscous liquid, at room temperature, to a reddish substance close to 80 °C. The reversibility of this optical effect was highlighted by the thermal stability of the Eu(iii) complex.

  10. Student Organizations--FBLA Projects and Activities Relating to Free Enterprise.

    ERIC Educational Resources Information Center

    Western Kentucky Univ., Bowling Green.

    This guide for Future Business Leaders of America and Phi Beta Lambda chapters describes 25 economic awareness projects/activities to create awareness of the free enterprise economic system. Introductory materials include suggestions for project planning and management and a worksheet for planning activities and projects. The projects/activities…

  11. Cooperative activation of the T-type CaV3.2 channel: interaction between Domains II and III.

    PubMed

    Demers-Giroux, Pierre-Olivier; Bourdin, Benoîte; Sauvé, Rémy; Parent, Lucie

    2013-10-11

    T-type CaV3 channels are important mediators of Ca(2+) entry near the resting membrane potential. Little is known about the molecular mechanisms responsible for channel activation. Homology models based upon the high-resolution structure of bacterial NaV channels predict interaction between the S4-S5 helix of Domain II (IIS4-S5) and the distal S6 pore region of Domain II (IIS6) and Domain III (IIIS6). Functional intra- and inter-domain interactions were investigated with a double mutant cycle analysis. Activation gating and channel kinetics were measured for 47 single mutants and 20 pairs of mutants. Significant coupling energies (ΔΔG(interact) ≥ 1.5 kcal mol(-1)) were measured for 4 specific pairs of mutants introduced between IIS4-S5 and IIS6 and between IIS4-S5 and IIIS6. In agreement with the computer based models, Thr-911 in IIS4-S5 was functionally coupled with Ile-1013 in IIS6 during channel activation. The interaction energy was, however, found to be stronger between Val-907 in IIS4-S5 and Ile-1013 in IIS6. In addition Val-907 was significantly coupled with Asn-1548 in IIIS6 but not with Asn-1853 in IVS6. Altogether, our results demonstrate that the S4-S5 and S6 helices from adjacent domains are energetically coupled during the activation of a low voltage-gated T-type CaV3 channel.

  12. Photochemical and DFT studies on DNA-binding ability and antibacterial activity of lanthanum(III)-phenanthroline complex

    NASA Astrophysics Data System (ADS)

    Niroomand, Sona; Khorasani-Motlagh, Mozhgan; Noroozifar, Meissam; Jahani, Shohreh; Moodi, Asieh

    2017-02-01

    The binding of the lanthanum(III) complex containing 1,10-phenanthroline (phen), [La(phen)3Cl3·OH2], to DNA is investigated by absorption and emission methods. This complex shows absorption decreasing in a charge transfer band, and fluorescence decrement when it binds to DNA. Electronic absorption spectroscopy (UV-Vis), fluorescence spectra, iodide quenching experiments, salt effect and viscosity measurements, ethidium bromide (EB) competition test, circular dichroism (CD) spectra as well as variable temperature experiments indicate that the La(III) complex binds to fish salmon (FS) DNA, presumably via groove binding mode. The binding constants (Kb) of the La(III) complex with DNA is (2.55 ± 0.02) × 106 M-1. Furthermore, the binding site size, n, the Stern-Volmer constant KSV and thermodynamic parameters; enthalpy change (ΔH0) and entropy change (ΔS0) and Gibb's free energy (ΔG0), are calculated according to relevant fluorescent data and the Van't Hoff equation. The La(III) complex has been screened for its antibacterial activities by the disc diffusion method. Also, in order to supplement the experimental findings, DFT computation and NBO analysis are carried out.

  13. Structure and membrane remodeling activity of ESCRT-III helical polymers

    SciTech Connect

    McCullough, John; Clippinger, Amy K.; Talledge, Nathaniel; Skowyra, Michael L.; Saunders, Marissa G.; Naismith, Teresa V.; Colf, Leremy A.; Afonine, Pavel; Arthur, Christopher; Sundquist, Wesley I.; Hanson, Phyllis I.; Frost, Adam

    2015-12-18

    The endosomal sorting complexes required for transport (ESCRT) proteins mediate fundamental membrane remodeling events that require stabilizing negative membrane curvature. These include endosomal intralumenal vesicle formation, HIV budding, nuclear envelope closure, and cytokinetic abscission. ESCRT-III subunits perform key roles in these processes by changing conformation and polymerizing into membrane-remodeling filaments. Here, we report the 4 angstrom resolution cryogenic electron microscopy reconstruction of a one-start, double-stranded helical copolymer composed of two different human ESCRT-III subunits, charged multivesicular body protein 1B (CHMP1B) and increased sodium tolerance 1 (IST1). The inner strand comprises “open” CHMP1B subunits that interlock in an elaborate domain-swapped architecture and is encircled by an outer strand of “closed” IST1 subunits. Unlike other ESCRT-III proteins, CHMP1B and IST1 polymers form external coats on positively curved membranes in vitro and in vivo. In conclusion, our analysis suggests how common ESCRT-III filament architectures could stabilize different degrees and directions of membrane curvature.

  14. Structure and membrane remodeling activity of ESCRT-III helical polymers.

    PubMed

    McCullough, John; Clippinger, Amy K; Talledge, Nathaniel; Skowyra, Michael L; Saunders, Marissa G; Naismith, Teresa V; Colf, Leremy A; Afonine, Pavel; Arthur, Christopher; Sundquist, Wesley I; Hanson, Phyllis I; Frost, Adam

    2015-12-18

    The endosomal sorting complexes required for transport (ESCRT) proteins mediate fundamental membrane remodeling events that require stabilizing negative membrane curvature. These include endosomal intralumenal vesicle formation, HIV budding, nuclear envelope closure, and cytokinetic abscission. ESCRT-III subunits perform key roles in these processes by changing conformation and polymerizing into membrane-remodeling filaments. Here, we report the 4 angstrom resolution cryogenic electron microscopy reconstruction of a one-start, double-stranded helical copolymer composed of two different human ESCRT-III subunits, charged multivesicular body protein 1B (CHMP1B) and increased sodium tolerance 1 (IST1). The inner strand comprises "open" CHMP1B subunits that interlock in an elaborate domain-swapped architecture and is encircled by an outer strand of "closed" IST1 subunits. Unlike other ESCRT-III proteins, CHMP1B and IST1 polymers form external coats on positively curved membranes in vitro and in vivo. Our analysis suggests how common ESCRT-III filament architectures could stabilize different degrees and directions of membrane curvature.

  15. Structure and membrane remodeling activity of ESCRT-III helical polymers

    DOE PAGES

    McCullough, John; Clippinger, Amy K.; Talledge, Nathaniel; ...

    2015-12-18

    The endosomal sorting complexes required for transport (ESCRT) proteins mediate fundamental membrane remodeling events that require stabilizing negative membrane curvature. These include endosomal intralumenal vesicle formation, HIV budding, nuclear envelope closure, and cytokinetic abscission. ESCRT-III subunits perform key roles in these processes by changing conformation and polymerizing into membrane-remodeling filaments. Here, we report the 4 angstrom resolution cryogenic electron microscopy reconstruction of a one-start, double-stranded helical copolymer composed of two different human ESCRT-III subunits, charged multivesicular body protein 1B (CHMP1B) and increased sodium tolerance 1 (IST1). The inner strand comprises “open” CHMP1B subunits that interlock in an elaborate domain-swapped architecturemore » and is encircled by an outer strand of “closed” IST1 subunits. Unlike other ESCRT-III proteins, CHMP1B and IST1 polymers form external coats on positively curved membranes in vitro and in vivo. In conclusion, our analysis suggests how common ESCRT-III filament architectures could stabilize different degrees and directions of membrane curvature.« less

  16. Sacramento River Flood Control Project, California, Mid-Valley Area, Phase III. Design Memorandum, Volume 1 of 2

    DTIC Science & Technology

    1995-08-01

    1944 and May 1950 and incorporated under Sacramento River and Major and Minor Tributaries. Although construction of the project was initiated in 1918...many of the levees were originally constructed by local interests prior to that time and subsequently modified and adopted as part of the project...6-1 6.02. Reconstruction Plans .. ................................... 6-2 6.03. Construction Considerations

  17. Adult Dyslexia and Attention Deficit Disorder in Finland--Project DyAdd: WAIS-III Cognitive Profiles

    ERIC Educational Resources Information Center

    Laasonen, Marja; Leppamaki, Sami; Tani, Pekka; Hokkanen, Laura

    2009-01-01

    The project Adult Dyslexia and Attention Deficit Disorder in Finland (Project DyAdd) compares adults (n = 119, 18-55 years) with dyslexia, attention-deficit/hyperactivity disorder (ADHD), dyslexia together with ADHD (comorbid), and healthy controls with neuropsychological, psychophysical, and biological methods. The focus of this article is on the…

  18. Evaluation of Title I ESEA Projects, 1971-1972. Volume III, Instructional Practices and Student Cognitive Performance.

    ERIC Educational Resources Information Center

    Prusso, Kenneth W.; And Others

    In this volume, ESEA Title I projects related to instructional practices and student cognitive performance, carried out in Philadelphia during 1971-1972, are evaluated. The six projects in this cluster are: Class for Mentally Retarded/Emotionally Distrubed Children; English as a Second Language; Improvement of Reading Skills (Reading Skills…

  19. Project Talented and Gifted, End of Grant Report. (ESEA Title III) Appendix IV: Part 2 of Two Bindings.

    ERIC Educational Resources Information Center

    1977

    Presented is a collection of instructional program units, or mini-workshops, developed by the coordinators and resource personnel working in Project Talented and Gifted and used by students participating in the project at the elementary and secondary level. Sections on each topic usually cover objectives and outlines of each session in the…

  20. Tartrazine modified activated carbon for the removal of Pb(II), Cd(II) and Cr(III).

    PubMed

    Monser, Lotfi; Adhoum, Nafaâ

    2009-01-15

    A two in one attempt for the removal of tartrazine and metal ions on activated carbon has been developed. The method was based on the modification of activated carbon with tartrazine then its application for the removal of Pb(II), Cd(II) and Cr(III) ions at different pH values. Tartrazine adsorption data were modelled using both Langmuir and Freundlich classical adsorption isotherms. The adsorption capacities qm were 121.3, 67 and 56.7mgg(-1) at initial pH values of 1.0, 6.0 and 10, respectively. The adsorption of tartrazine onto activated carbon followed second-order kinetic model. The equilibrium time was found to be 240min at pH 1.0 and 120min at pH 10 for 500mgL(-1) tartrazine concentration. A maximum removal of 85% was obtained after 1h of contact time. The presence of tartrazine as modifier enhances attractive electrostatic interactions between metal ions and carbon surface. The adsorption capacity for Pb(II), Cd(II) and Cr(III) ions has been improved with respect to non-modified carbon reaching a maximum of 140%. The adsorption capacity was found to be a pH dependent for both modified and non-modified carbon with a greater adsorption at higher pH values except for Cr(III). The enhancement percent of Pb(II), Cd(II) and Cr(III) at different pH values was varied from 28% to 140% with respect to non-modified carbon. The amount of metal ions adsorbed using static regime was 11-40% higher than that with dynamic mode. The difference between adsorption capacities could be attributed to the applied flow rate.

  1. Computer-Based Testing System. Project STEEL. A Special Project To Develop and Implement a Computer-Based Special Teacher Education and Evaluation Laboratory. Volume III. Final Report.

    ERIC Educational Resources Information Center

    Frick, Theodore W.; And Others

    The document is part of the final report on Project STEEL (Special Teacher Education and Evaluation Laboratory) intended to extend the utilization of technology in the training of preservice special education teachers. This volume focuses on the third of four project objectives, the development and implementation of a computer-based testing…

  2. Comparative activities of the triterpene saponin maesabalide III and liposomal amphotericin B (AmBisome) against Leishmania donovani in hamsters.

    PubMed

    Maes, Louis; Germonprez, Nils; Quirijnen, Ludo; Van Puyvelde, Luc; Cos, Paul; Vanden Berghe, Dirk

    2004-06-01

    Maesabalide III (MB-III), an oleane triterpene saponin isolated from the Vietnamese plant Maesa balansae, is a new antileishmanial lead compound whose activity against Leishmania donovani (MHOM/ET/67/L82) in groups of five golden hamsters was evaluated after administration of a single subcutaneous dose on either day 1 (prophylactic treatment) or day 28 (curative treatment) after infection. Liposomal amphotericin B (AmBisome), administered intravenously at 5 mg/kg of body weight, was used as the reference drug. Amastigote burdens in liver, spleen, and bone marrow were determined either 7 days (early effects) or 56 days (late effects) after treatment. Prophylactic administration of MB-III at 0.2 mg/kg reduced liver amastigote burdens by 99.8 and 83% within 7 and 56 days after treatment, respectively. In the latter group, however, all animals became ill and some died. Both MB-III at 0.8 mg/kg and liposomal amphotericin B were 100% effective against liver stages, but clearance from the spleen and bone marrow was not achieved. Curative administration of MB-III at 0.2 and 0.4 mg/kg was not protective, as no survivors were left at the termination of the experiment on day 84. Despite the high level of reduction of the liver amastigote burden after treatment with MB-III at 0.8 mg/kg (94.2%) or liposomal amphotericin B (99.4%), clinical protection could not be obtained in either group, with two deaths occurring and the residual liver burdens persisting. It is concluded that administration of a single dose of MB-III at 0.8 mg/kg has efficacy potential comparable to that of a single dose of liposomal amphotericin B at 5 mg/kg and is therefore considered a promising new antileishmanial lead compound. However, multiple-dose pharmacological, toxicological, and pharmacokinetic studies are still needed before it can become a valid drug candidate for development.

  3. Synthesis of Succinimide-Containing Chromones, Naphthoquinones, and Xanthones under Rh(III) Catalysis: Evaluation of Anticancer Activity.

    PubMed

    Han, Sang Hoon; Kim, Saegun; De, Umasankar; Mishra, Neeraj Kumar; Park, Jihye; Sharma, Satyasheel; Kwak, Jong Hwan; Han, Sangil; Kim, Hyung Sik; Kim, In Su

    2016-12-16

    The weakly coordinating ketone group directed C-H functionalizations of chromones, 1,4-naphthoquinones, and xanthones with various maleimides under rhodium(III) catalysis are described. These protocols efficiently provide a range of succinimide-containing chromones, naphthoquinones, and xanthones with excellent site selectivity and functional group compatibility. All synthetic compounds were screened for in vitro anticancer activity against human breast adenocarcinoma cell lines (MCF-7). In particular, compounds 7aa and 7ca with a naphthoquinone scaffold were found to be highly cytotoxic, with an activity competitive with anticancer agent doxorubicin.

  4. Activity and regulation by growth factors of calmodulin-dependent protein kinase III (elongation factor 2-kinase) in human breast cancer

    PubMed Central

    Parmer, T G; Ward, M D; Yurkow, E J; Vyas, V H; Kearney, T J; Hait, W N

    1999-01-01

    Calmodulin-dependent protein kinase III (CaM kinase III, elongation factor-2 kinase) is a unique member of the Ca2+/CaM-dependent protein kinase family. Activation of CaM kinase III leads to the selective phosphorylation of elongation factor 2 (eEF-2) and transient inhibition of protein synthesis. Recent cloning and sequencing of CaM kinase III revealed that this enzyme represents a new superfamily of protein kinases. The activity of CaM kinase III is selectively activated in proliferating cells; inhibition of the kinase blocked cells in G0/G1-S and decreased viability. To determine the significance of CaM kinase III in breast cancer, we measured the activity of the kinase in human breast cancer cell lines as well as in fresh surgical specimens. The specific activity of CaM kinase III in human breast cancer cell lines was equal to or greater than that seen in a variety of cell lines with similar rates of proliferation. The specific activity of CaM kinase III was markedly increased in human breast tumour specimens compared with that of normal adjacent breast tissue. The activity of this enzyme was regulated by breast cancer mitogens. In serum-deprived MDA-MB-231 cells, the combination of insulin-like growth factor I (IGF-I) and epidermal growth factor (EGF) stimulated cell proliferation and activated CaM kinase III to activities observed in the presence of 10% serum. Inhibition of enzyme activity blocked cell proliferation induced by growth factors. In MCF-7 cells separated by fluorescence-activated cell sorting, CaM kinase III was increased in S-phase over that of other phases of the cell cycle. In summary, the activity of Ca2+/CaM-dependent protein kinase III is controlled by breast cancer mitogens and appears to be constitutively activated in human breast cancer. These results suggest that CaM kinase III may contribute an important link between growth factor/receptor interactions, protein synthesis and the induction of cellular proliferation in human breast

  5. Quality Assurance Project Plan for Facility Effluent Monitoring Plan activities

    SciTech Connect

    Nickels, J.M.

    1991-06-01

    This Quality Assurance Project Plan addresses the quality assurance requirements for the Facility Monitoring Plans of the overall site-wide environmental monitoring plan. This plan specifically applies to the sampling and analysis activities and continuous monitoring performed for all Facility Effluent Monitoring Plan activities conducted by Westinghouse Hanford Company. It is generic in approach and will be implemented in conjunction with the specific requirements of individual Facility Effluent Monitoring Plans. This document is intended to be a basic road map to the Facility Effluent Monitoring Plan documents (i.e., the guidance document for preparing Facility Effluent Monitoring Plans, Facility Effluent Monitoring Plan determinations, management plan, and Facility Effluent Monitoring Plans). The implementing procedures, plans, and instructions are appropriate for the control of effluent monitoring plans requiring compliance with US Department of Energy, US Environmental Protection Agency, state, and local requirements. This Quality Assurance Project Plan contains a matrix of organizational responsibilities, procedural resources from facility or site manuals used in the Facility Effluent Monitoring Plans, and a list of the analytes of interest and analytical methods for each facility preparing a Facility Effluent Monitoring Plan. 44 refs., 1 figs., 2 tabs.

  6. SERS Activity of Silver Nanoparticles Functionalized with A Desferrioxamine B Derived Ligand for FE(III) Binding and Sensing

    NASA Astrophysics Data System (ADS)

    Galinetto, P.; Taglietti, A.; Pasotti, L.; Pallavicini, P.; Dacarro, G.; Giulotto, E.; Grandi, M. S.

    2016-01-01

    We report the SERS activity of colloidal silver nanoparticles functionalized with a ligand, derived from the siderophore desferrioxamine B (desferal, DFO), an iron chelator widely used in biological and medical applications. The ligand was equipped with a sulfur-containing moiety to ensure optimal binding with silver surfaces. By means of Raman and SERS effects we monitored the route of material preparation from the modified DFO-S molecule to the colloidal aggregates. The results indicate that the functionalization of the chelating agent does not affect its binding ability towards Fe(III). The resulting functionalized silver nanoparticles are a promising SERS tag for operation in biological environments. The Fe-O stretching signature, arising when DFO-S grafted to silver nanoparticles binds Fe(III), could provide a tool for cation sensing in solution.

  7. Synthesis, spectroscopic characterization and antibacterial activity of antimony(III) bis(dialkyldithiocarbamato)alkyldithiocarbonates

    NASA Astrophysics Data System (ADS)

    Chauhan, H. P. S.; Bakshi, Abhilasha; Bhatiya, Sumit

    2011-10-01

    Some mixed sulfur donor ligand complexes of antimony(III) of the general formula [(R 2NCS 2)] 2SbS 2COR' where R = CH 3, C 2H 5 and R' = Me, Et, Pr n, Pr i, Bu n and Bu i have been synthesized by the reaction of antimony(III) bis(dialkyldithiocarbamate) chloride with potassium organodithiocarbonate in an equimolar ratio by stirring at room temperature in benzene/CS 2 mixture. These complexes have been characterized by physicochemical [elemental analysis, melting points and molecular weight determinations] and spectral [UV, IR, Far-IR, NMR ( 1H and 13C), FAB + mass and powder X-ray diffraction] studies. Free ligands and synthesized complexes have also been screened against different bacterial strains and results obtained made it desirable to delineate a comparison between free ligands, standard drug used and synthesized complexes.

  8. Synthesis of the Tolerance-Inducing Oligosaccharide Lacto-N-Fucopentaose III Bearing an Activated Linker

    PubMed Central

    Zhang, Junfeng; Zou, Lu; Lowary, Todd L

    2013-01-01

    A concise synthetic route to an immunomodulatory pentasaccharide, lacto-N-fucopentaose III (1) and its corresponding human serum albumin conjugate, is described. Key transformations of the strategy include two highly regio- and stereoselective glycosylations for the construction of disaccharide 10 and pentasaccharide 12, a Birch reduction for deprotection of benzyl ethers, and a UV-promoted radical addition of a thiol to an alkene for modification of the aglycone. PMID:24551556

  9. Amber light-emitting diode comprising a group III-nitride nanowire active region

    DOEpatents

    Wang, George T.; Li, Qiming; Wierer, Jr., Jonathan J.; Koleske, Daniel

    2014-07-22

    A temperature stable (color and efficiency) III-nitride based amber (585 nm) light-emitting diode is based on a novel hybrid nanowire-planar structure. The arrays of GaN nanowires enable radial InGaN/GaN quantum well LED structures with high indium content and high material quality. The high efficiency and temperature stable direct yellow and red phosphor-free emitters enable high efficiency white LEDs based on the RGYB color-mixing approach.

  10. 76 FR 73615 - FFP Project 91 LLC; Riverbank Hydro No. 23 LLC; Lock Hydro Friends Fund III; Notice of Competing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-29

    ... hydropower project at the U.S. Army Corps of Engineers' (Corps) Kentucky River Lock & Dam No. 10, located on... Support. Although the Commission strongly encourages electronic filing, documents may also be...

  11. An Overview of SAGE III Validation Activities during the SOLVE-2/VINTERSOL Campaigns

    NASA Astrophysics Data System (ADS)

    Trepte, C.

    2003-12-01

    A major goal of the SAGE III Ozone Loss and Validation Experiment (SOLVE) II and the Validation of International Satellites and Study of Ozone Loss (VINTERSOL) field experiments is to support validation studies for a new generation of satellite experiments that had been recently placed in orbit. The Stratospheric Aerosol and Gas Experiment (SAGE) III instrument was launched in December 2001 on board a Russian Meteor 3M satellite to provide information on the behavior of ozone, aerosols, water vapor and other trace species at high and mid latitudes in the upper troposphere and stratosphere. The instrument supports an advanced design from its predecessor with added spectral coverage and resolution and greater dynamic range that allows for observations during solar and lunar occultations as well as measurements of scattered light along the limb. During the SOLVE-2/VINTERSOL campaigns, a comprehensive set of correlative measurements was obtained from a network of ground-based instruments and the launch small balloon payloads from high latitude sites, the launch of several large balloon payloads and multiple rockets from Esrange, Sweden and flights of the NASA DC-8, the DLR Falcon, and the M55 Geophysica aircraft based from Kiruna, Sweden. These measurements provide an opportunity to assess biases between instrument techniques and to help interpret satellite observations in a variety of meteorological settings. This presentation will highlight comparison findings for the SAGE III satellite experiment from the array of observations collected during the SOLVE-2/VINTERSOL campaigns.

  12. Bipartite recognition of target RNAs activates DNA cleavage by the Type III-B CRISPR–Cas system

    PubMed Central

    Elmore, Joshua R.; Sheppard, Nolan F.; Ramia, Nancy; Deighan, Trace; Li, Hong; Terns, Rebecca M.; Terns, Michael P.

    2016-01-01

    CRISPR–Cas systems eliminate nucleic acid invaders in bacteria and archaea. The effector complex of the Type III-B Cmr system cleaves invader RNAs recognized by the CRISPR RNA (crRNA ) of the complex. Here we show that invader RNAs also activate the Cmr complex to cleave DNA. As has been observed for other Type III systems, Cmr eliminates plasmid invaders in Pyrococcus furiosus by a mechanism that depends on transcription of the crRNA target sequence within the plasmid. Notably, we found that the target RNA per se induces DNA cleavage by the Cmr complex in vitro. DNA cleavage activity does not depend on cleavage of the target RNA but notably does require the presence of a short sequence adjacent to the target sequence within the activating target RNA (rPAM [RNA protospacer-adjacent motif]). The activated complex does not require a target sequence (or a PAM) in the DNA substrate. Plasmid elimination by the P. furiosus Cmr system also does not require the Csx1 (CRISPR-associated Rossman fold [CARF] superfamily) protein. Plasmid silencing depends on the HD nuclease and Palm domains of the Cmr2 (Cas10 superfamily) protein. The results establish the Cmr complex as a novel DNA nuclease activated by invader RNAs containing a crRNA target sequence and a rPAM. PMID:26848045

  13. Global projects and Astronomy awareness activities in Nepal

    NASA Astrophysics Data System (ADS)

    Gautam, Suman

    2015-08-01

    Modern astronomy is a crowning achievement of human civilization which inspires teenagers to choose career in science and technology and is a stable of adult education. It is a unique and cost effective tool for furthering sustainable global development because of its technological, scientific and cultural dimensions which allow us to reach with the large portion of the community interact with children and inspire with our wonderful cosmos.Using astronomy to stimulate quality and inspiring education for disadvantaged children is an important goal of Nepal Astronomical Society (NASO) since its inception. NASO is carrying out various awareness activities on its own and in collaboration with national and international organizations like Central Department of Physics Tribhuvan University (TU), International astronomical Union (IAU), Department of Physics Prithvi Narayan Campus Pokhara, Nepal academy of science and technology (NAST), Global Hands on Universe (GHOU), EU- UNAWE and Pokhara Astronomical Society (PAS) to disseminate those activities for the school children and teachers in Nepal. Our experiences working with kids, students, teachers and public in the field of universe Awareness Activities for the school children to minimize the abstruse concept of astronomy through some practical approach and the project like Astronomy for the visually impaired students, Galileo Teacher Training program and International School for young astronomers (ISYA) outskirts will be explained which is believed to play vital role in promoting astronomy and space science activities in Nepal.

  14. Crossed and uncrossed projections to the cat sacrocaudal spinal cord: III. Axons expressing calcitonin gene-related peptide immunoreactivity.

    PubMed

    Ritz, L A; Murray, C R; Foli, K

    2001-10-01

    We have investigated the projection patterns of peptidergic small-diameter primary afferent fibers to the cat sacrocaudal spinal cord, a region associated with midline structures of the lower urogenital system and of the tail. Calcitonin gene-related peptide (CGRP)-immunoreactive (CGRP-IR) primary afferent fibers were observed within the superficial laminae, rostrally as the typical inverted U-shaped band that capped the separate dorsal horns (S1 to rostral S2) and caudally as a broad band that spanned the entire mediolateral extent of the fused dorsal horns (caudal S2 and caudal). Within the dorsal gray commissure, labeling was seen as a periodic vertical, midline band. CGRP-IR labeling was prevalent in an extensive mediolateral distribution at the base of the dorsal horn, originating from both lateral and medial collateral bundles that extend from the superficial dorsal horn. Some bundles, in part traveling within the dorsal commissure, conspicuously crossed the midline. In addition to the robust projection to the superficial dorsal horn, there was a more extensive distribution of CGRP-IR fibers within the deeper portions of the cat sacrocaudal dorsal horn than has been reported for other regions of the cat spinal cord. Presumably, these deep projections convey visceral information to projection or segmental neurons at the neck of the dorsal horn and in the region of the central canal. This deep distribution overlaps the reported projections of the pelvic and pudendal nerves. In addition, the contralateral projections of CGRP-IR fibers may form an anatomical substrate of the bilateral receptive fields for selective dorsal horn neurons. The density and variety of CGRP-IR projection patterns is a reflection of the functional attributes of the innervated structures.

  15. Activation of Type I and III Interferon Response by Mitochondrial and Peroxisomal MAVS and Inhibition by Hepatitis C Virus

    PubMed Central

    Bender, Silke; Reuter, Antje; Eberle, Florian; Einhorn, Evelyne; Binder, Marco; Bartenschlager, Ralf

    2015-01-01

    Sensing viruses by pattern recognition receptors (PRR) triggers the innate immune system of the host cell and activates immune signaling cascades such as the RIG-I/IRF3 pathway. Mitochondrial antiviral-signaling protein (MAVS, also known as IPS-1, Cardif, and VISA) is the crucial adaptor protein of this pathway localized on mitochondria, peroxisomes and mitochondria-associated membranes of the endoplasmic reticulum. Activation of MAVS leads to the production of type I and type III interferons (IFN) as well as IFN stimulated genes (ISGs). To refine the role of MAVS subcellular localization for the induction of type I and III IFN responses in hepatocytes and its counteraction by the hepatitis C virus (HCV), we generated various functional and genetic knock-out cell systems that were reconstituted to express mitochondrial (mito) or peroxisomal (pex) MAVS, exclusively. Upon infection with diverse RNA viruses we found that cells exclusively expressing pexMAVS mounted sustained expression of type I and III IFNs to levels comparable to cells exclusively expressing mitoMAVS. To determine whether viral counteraction of MAVS is affected by its subcellular localization we employed infection of cells with HCV, a major causative agent of chronic liver disease with a high propensity to establish persistence. This virus efficiently cleaves MAVS via a viral protease residing in its nonstructural protein 3 (NS3) and this strategy is thought to contribute to the high persistence of this virus. We found that both mito- and pexMAVS were efficiently cleaved by NS3 and this cleavage was required to suppress activation of the IFN response. Taken together, our findings indicate comparable activation of the IFN response by pex- and mitoMAVS in hepatocytes and efficient counteraction of both MAVS species by the HCV NS3 protease. PMID:26588843

  16. Computational Mechanistic Study of Redox-Neutral Rh(III)-Catalyzed C-H Activation Reactions of Arylnitrones with Alkynes: Role of Noncovalent Interactions in Controlling Selectivity.

    PubMed

    Xing, Yang-Yang; Liu, Jian-Biao; Tian, Ying-Ying; Sun, Chuan-Zhi; Huang, Fang; Chen, De-Zhan

    2016-11-23

    The mechanism of redox-neutral Rh(III)-catalyzed coupling reactions of arylnitrones with alkynes was investigated by density functional theory (DFT) calculations. The free energy profiles associated with the catalytic cycle, involving C(sp(2))-H activation, insertion of alkyne, transfer of O atom, cyclization and protodemetalation, are presented and analyzed. An overwhelming preference for alkyne insertion into Rh-C over Rh-O is observed among all pathways, and the most favorable route is determined. The pivalate-assisted C-H activation step is turnover-limiting, and the cyclization step determines the diastereoselectivity of the reaction, with the stereoselectivity arising mainly from the difference of noncovalent interactions in key transition states. The detailed mechanism of O atom transfer, Rh(III)-Rh(I)-Rh(III) versus Rh(III)-Rh(V)-Rh(III) cycle, is discussed.

  17. An Empirical Investigation of Pre-Project Partnering Activities on Project Performance in the Software Industry

    ERIC Educational Resources Information Center

    Proffitt, Curtis K.

    2012-01-01

    Project failure remains a challenge within the software development field especially during the early stages of the IT project development. Despite the herculean efforts by project managers and organizations to identify and offset problems, projects remain plagued with issues. If these challenges are not mitigated, to a successful degree,…

  18. Present status of some technological activities supporting the MOLCARE project

    SciTech Connect

    Torazza, A.; Rocchini, G.; Scagliotti, M.

    1996-12-31

    The development of MCFC stack technology is carried out at Ansaldo Ricerche in the framework of the MOLCARE project, a cooperation with Spanish companies under a partial UE funding, while a specific research program concerning the physico-chemical characterization of materials is performed jointly by CISE and ENEL. The project includes the development, the construction and the testing of a full scale 100 kW prototype, the assessment of stack technology on subscale stacks, the mathematical modelling of the MCFC based plants and the basic researches. The aim of the basic researches, carried out on single cells, is to improve the effectiveness and durability of both the active and the hardware materials. The Ansaldo stack technology is based on external manifolding. The full scale 100 kW prototype will be integrated with the sensible heat reformer and other ancillary equipments according to the {open_quote}Compact Unit (CU){close_quotes} concept. These technical choices stress requirements for manifold gasket configuration. electrolyte migration control, {Delta}p management and porous component compaction.

  19. Self-service for software development projects and HPC activities

    NASA Astrophysics Data System (ADS)

    Husejko, M.; Høimyr, N.; Gonzalez, A.; Koloventzos, G.; Asbury, D.; Trzcinska, A.; Agtzidis, I.; Botrel, G.; Otto, J.

    2014-05-01

    This contribution describes how CERN has implemented several essential tools for agile software development processes, ranging from version control (Git) to issue tracking (Jira) and documentation (Wikis). Running such services in a large organisation like CERN requires many administrative actions both by users and service providers, such as creating software projects, managing access rights, users and groups, and performing tool-specific customisation. Dealing with these requests manually would be a time-consuming task. Another area of our CERN computing services that has required dedicated manual support has been clusters for specific user communities with special needs. Our aim is to move all our services to a layered approach, with server infrastructure running on the internal cloud computing infrastructure at CERN. This contribution illustrates how we plan to optimise the management of our of services by means of an end-user facing platform acting as a portal into all the related services for software projects, inspired by popular portals for open-source developments such as Sourceforge, GitHub and others. Furthermore, the contribution will discuss recent activities with tests and evaluations of High Performance Computing (HPC) applications on different hardware and software stacks, and plans to offer a dynamically scalable HPC service at CERN, based on affordable hardware.

  20. [Various aspects of cognitive activities of schizophrenics. III. Maladjustment assimilation/accommodation and generalization of reasoning].

    PubMed

    Blein, G; Azorin, J M; Vollrath, J L; Andréoli, A; Tissot, R

    1987-05-01

    Paranoid schizophrenics are unable to balance affirmation and negation. Using the terms of Jean Piaget, they therefore only partially achieve the "équilibrations majorantes" of level II and never those of level III. In their thinking, they have access to the "généralisations inductives" (often excessive ones), but rarely to the "généralisations constructives complétives". They do not have access to the "généralisations synthétisantes" or to the feeling that logic is necessary. They oscillate between the positivist need to measure or verify and absolute beliefs issued from magical thinking.

  1. Symptomatic and neuroprotective effects following activation of nigral group III metabotropic glutamate receptors in rodent models of Parkinson's disease

    PubMed Central

    Austin, PJ; Betts, MJ; Broadstock, M; O'Neill, MJ; Mitchell, SN; Duty, S

    2010-01-01

    Background and purpose: Increased glutamatergic innervation of the substantia nigra pars reticulata (SNpr) and pars compacta (SNpc) may contribute to the motor deficits and neurodegeneration, respectively, in Parkinson's disease (PD). This study aimed to establish whether activation of pre-synaptic group III metabotropic glutamate (mGlu) receptors reduced glutamate release in the SN, and provided symptomatic or neuroprotective relief in animal models of PD. Experimental approach: Broad-spectrum group III mGlu receptor agonists, O-phospho-l-serine (l-SOP) and l-2-amino-4-phosphonobutyrate (l-AP4), were assessed for their ability to inhibit KCl-evoked [3H]-d-aspartate release in rat nigral prisms or inhibit KCl-evoked endogenous glutamate release in the SNpr in vivo using microdialysis. Reversal of akinesia in reserpine-treated rats was assessed following intranigral injection of l-SOP and l-AP4. Finally, the neuroprotective effect of 7 days' supra-nigral treatment with l-AP4 was examined in 6-hydroxydopamine (6-OHDA)-lesioned rats. Key results: l-SOP and l-AP4 inhibited [3H]-d-aspartate release by 33 and 44% respectively. These effects were blocked by the selective group III mGlu antagonist (RS)-α-cyclopropyl-4-phosphonophenylglycine (CPPG). l-SOP also reduced glutamate release in the SNpr in vivo by 48%. Injection of l-SOP and l-AP4 into the SNpr reversed reserpine-induced akinesia. Following administration above the SNpc, l-AP4 provided neurochemical, histological and functional protection against 6-OHDA lesion of the nigrostriatal tract. Pretreatment with CPPG inhibited these effects. Conclusions and implications: These findings highlight group III mGlu receptors in the SN as potential targets for providing both symptomatic and neuroprotective relief in PD, and indicate that inhibition of glutamate release in the SN may underlie these effects. PMID:20649576

  2. Active Spacecraft Potential Control: Results From the Double Star Project

    NASA Astrophysics Data System (ADS)

    Torkar, K.; Fazakerley, A.; Steiger, W.

    2006-10-01

    The ion emitter instrument "active spacecraft potential control" (ASPOC) has been used successfully in several magnetospheric missions including the European Space Agency Cluster Project. An improved version has been developed for the equatorial spacecraft of the Chinese-European Double Star mission (TC-1) launched in December 2003. The modifications include a new design of the ion emitter modules. As a result, higher currents than in previous missions can be achieved. The main objective of the investigation is the reduction of positive spacecraft potential in order to minimize perturbations to the plasma measurements onboard, in particular to the plasma electron instrument PEACE. These data show an almost complete suppression of photoelectrons when ASPOC is emitting at 30- to 50-muA beam current. The angular distribution of the electrons in the presence of the ion beam is investigated in detail. The measurement of ambient electron distributions is highly improved.

  3. Efficient active depth sensing by laser speckle projection system

    NASA Astrophysics Data System (ADS)

    Yin, Xuanwu; Wang, Guijin; Shi, Chenbo; Liao, Qingmin

    2014-01-01

    An active depth sensing approach by laser speckle projection system is proposed. After capturing the speckle pattern with an infrared digital camera, we extract the pure speckle pattern using a direct-global separation method. Then the pure speckles are represented by Census binary features. By evaluating the matching cost and uniqueness between the real-time image and the reference image, robust correspondences are selected as support points. After that, we build a disparity grid and propose a generative graphical model to compute disparities. An iterative approach is designed to propagate the messages between blocks and update the model. Finally, a dense depth map can be obtained by subpixel interpolation and transformation. The experimental evaluations demonstrate the effectiveness and efficiency of our approach.

  4. Construct Validity of the Chinese Version of the Activities of Daily Living Rating Scale III in Patients with Schizophrenia

    PubMed Central

    Chiu, En-Chi; Lee, Yen; Lai, Kuan-Yu; Kuo, Chian-Jue; Lee, Shu-Chun; Hsieh, Ching-Lin

    2015-01-01

    Background The Chinese version of the Activities of Daily Living Rating Scale III (ADLRS-III), which has 10 domains, is commonly used for assessing activities of daily living (ADL) in patients with schizophrenia. However, construct validity (i.e., unidimensionality) for each domain of the ADLRS-III is unknown, limiting the explanations of the test results. Purpose This main purpose of this study was to examine unidimensionality of each domain in the ADLRS-III. We also examined internal consistency and ceiling/floor effects in patients with schizophrenia. Methods From occupational therapy records, we obtained 304 self-report data of the ADLRS-III. Confirmatory factor analysis (CFA) was conducted to examine the 10 one-factor structures. If a domain showed an insufficient model fit, exploratory factor analysis (EFA) was performed to investigate the factor structure and choose one factor representing the original construct. Internal consistency was examined using Cronbach’s alpha (α). Ceiling and floor effects were determined by the percentage of patients with the maximum and minimum scores in each domain, respectively. Results CFA analyses showed that 4 domains (i.e., leisure, picture recognition, literacy ability, communication tools use) had sufficient model fits. These 4 domains had acceptable internal consistency (α = 0.79-0.87) and no ceiling/floor effects, except the leisure domain which had a ceiling effect. The other 6 domains showed insufficient model fits. The EFA results showed that these 6 domains were two-factor structures. Conclusion The results supported unidimensional constructs of the leisure, picture recognition, literacy ability, and communication tool uses domains. The sum scores of these 4 domains can be used to represent their respective domain-specific functions. Regarding the 6 domains with insufficient model fits, we have explained the two factors of each domain and chosen one factor to represent its original construct. Future users may

  5. Oxidative Stress and Antimicrobial Activity of Chromium(III) and Ruthenium(II) Complexes on Staphylococcus aureus and Escherichia coli

    PubMed Central

    Páez, Paulina L.; Bazán, Claudia M.; Bongiovanni, María E.; Toneatto, Judith; Albesa, Inés; Becerra, María C.; Argüello, Gerardo A.

    2013-01-01

    The prevalence of antibiotic resistance has resulted in the need for new approaches to be developed to combat previously easily treatable infections. The main aim of this work was to establish the potential of the synthetic α-diimine chromium(III) and ruthenium(II) complexes (where the α-diimine ligands are bpy = 2,2-bipyridine, phen = 1,10-phenanthroline, and dppz = dipyrido[3,2-a:2′,3′-c]-phenazine) like [Cr(phen)3]3+, [Cr(phen)2(dppz)]3+, [Ru(phen)3]2+, and [Ru(bpy)3]2+ as antibacterial agents by generating oxidative stress. The [Cr(phen)3]3+ and [Cr(phen)2(dppz)]3+ complexes showed activity against Gram positive and Gram negative bacteria with minimum inhibitory concentrations (MICs) ranging from 0.125 μg/mL to 1 μg/mL, while [Ru(phen)3]2+ and [Ru(bpy)3]2+ do not exhibit antimicrobial activity against the two bacterial genera studied at the concentration range used. When ciprofloxacin was combined with [Cr(phen)3]3+ for the inhibition of Staphylococcus aureus and Escherichia coli, an important synergistic effect was observed, FIC 0.066 for S. aureus and FIC 0.064 for E. coli. The work described here shows that chromium(III) complexes are bactericidal for S. aureus and E. coli. Our results indicate that α-diimine chromium(III) complexes may be interesting to open new paths for metallodrug chemotherapy against different bacterial genera since some of these complexes have been found to exhibit remarkable antibacterial activities. PMID:24093107

  6. Culture and Power in Practice: Cultural Democracy and the Family Support Movement. Best Practices Project Commissioned Paper III.

    ERIC Educational Resources Information Center

    Akinyela, Makungu M.

    The Best Practices Project of the Family Resource Coalition of American (FRCA) began in 1991 with the aim of meeting the need for better definition and articulation of what constitutes best practice in family support programs. This monograph, the third in a series of four, reports on some of the ideas about cultural democracy which have generated…

  7. Comprehensive Study of Educational Technology Programs Authorized from 1989-1992. Volume III: Level II Model Technology School Projects.

    ERIC Educational Resources Information Center

    Far West Lab. for Educational Research and Development, San Francisco, CA.

    This report, the third in a series of six, evaluates the 10 school districts that received grants from the California Department of Education to develop Level II Model Technology School (MTS) Projects intended to enhance instruction and student learning through a combination of curriculum improvement and integration of technology within a single…

  8. Pore-forming Activity of the Escherichia coli Type III Secretion System Protein EspD*

    PubMed Central

    Chatterjee, Abhishek; Caballero-Franco, Celia; Bakker, Dannika; Totten, Stephanie; Jardim, Armando

    2015-01-01

    Enterohemorrhagic Escherichia coli is a causative agent of gastrointestinal and diarrheal diseases. Pathogenesis associated with enterohemorrhagic E. coli involves direct delivery of virulence factors from the bacteria into epithelial cell cytosol via a syringe-like organelle known as the type III secretion system. The type III secretion system protein EspD is a critical factor required for formation of a translocation pore on the host cell membrane. Here, we show that recombinant EspD spontaneously integrates into large unilamellar vesicle (LUV) lipid bilayers; however, pore formation required incorporation of anionic phospholipids such as phosphatidylserine and an acidic pH. Leakage assays performed with fluorescent dextrans confirmed that EspD formed a structure with an inner diameter of ∼2.5 nm. Protease mapping indicated that the two transmembrane helical hairpin of EspD penetrated the lipid layer positioning the N- and C-terminal domains on the extralumenal surface of LUVs. Finally, a combination of glutaraldehyde cross-linking and rate zonal centrifugation suggested that EspD in LUV membranes forms an ∼280–320-kDa oligomeric structure consisting of ∼6–7 subunits. PMID:26324713

  9. Proton and gallium(III) binding properties of a biologically active salicylidene acylhydrazide.

    PubMed

    Hakobyan, Shoghik; Boily, Jean-François; Ramstedt, Madeleine

    2014-09-01

    Bacterial biofilm formation causes a range of problems in our society, especially in health care. Salicylidene acylhydrazides (hydrazones) are promising antivirulence drugs targeting secretion systems used during bacterial infection of host cells. When mixed with the gallium ion they become especially potent as bacterial and biofilm growth-suppressing agents, although the mechanisms through which this occurs are not fully understood. At the base of this uncertainty lies the nature of hydrazone-metal interactions. This study addresses this issue by resolving the equilibrium speciation of hydrazone-gallium aqueous solutions. The protonation constants of the target 2-oxo-2-[N-(2,4,6-trihydroxy-benzylidene)-hydrazino]-acetamide (ME0163) hydrazone species and of its 2,4,6-trihydroxybenzaldehyde and oxamic acid hydrazide building blocks were determined by UV-visible spectrophotometry to achieve this goal. These studies show that the hydrazone is an excessively strong complexing agent for gallium and that its antivirulence properties are predominantly ascribed to monomeric 1:1Ga-ME0163 complexes of various Ga hydrolysis and ME0163 protonation states. The chelation of Ga(III) to the hydrazone also increased the stability of the compounds against acid-induced hydrolysis, making this group of compounds very interesting for biological applications where the Fe-antagonist action of both Ga(III) and the hydrazone can be combined for enhanced biological effect.

  10. The Effect of Project-Based Activities on Intermediate EFL Students' Reading Comprehension Ability

    ERIC Educational Resources Information Center

    Shiraz, Mona Poorverdi; Larsari, Ebrahim Ezati

    2014-01-01

    The present study investigates the relationship between the use of Project-based activities and intermediate EFL students' reading comprehension. The study addresses the questions of whether students' reading comprehension differs after implementing Project-based activities, and whether different projects lead to different degrees of reading…

  11. Idaho Water Rental Pilot Project probability/coordination study resident fish and wildlife impacts, Phase III. Annual report

    SciTech Connect

    Leitzinger, E.

    1996-09-01

    Phase III began in 1995 with the overall goal of quantifying changes in resident fish habitat in the Snake River basin upstream of Brownlee Reservoir resulting from the release of salmon flow augmentation water. Existing data, in the form of weighted usable area versus flow relationships, were used to estimate habitat changes for white sturgeon (Acipenser transmontanus) and rainbow trout (Oncorhynchus mykiss)in the Snake River between C.J. Strike Dam and Brownlee pool. The increased flows resulted in increased white sturgeon habitat for most life stages. Rainbow trout adult and spawning habitat increased while juvenile and fry habitat generally decreased. Whether or not these short term increases in habitat result in long term benefits to the fish populations has yet to be determined.

  12. Idaho Water Rental Pilot Project Probability/Coordination Study Resident Fish and Wildlife Impact Phase III, 1995 Annual Report.

    SciTech Connect

    Leitzinger, Eric J.

    1996-09-01

    Phase III began in 1995 with the overall goal of quantifying changes in resident fish habitat in the Snake River basin upstream of Brownlee Reservoir resulting from the release of salmon flow augmentation water. Existing data, in the form of weighted usable area versus flow relationships, were used to estimate habitat changes for white sturgeon (Acipenser transmontanus) and rainbow trout (Oncorhynchus mykiss) in the Snake River between C.J. Strike Dam and Brownlee pool. The increased flows resulted in increased white sturgeon habitat for most life stages. Rainbow trout adult and spawning habitat increased while juvenile and fry habitat generally decreased. Whether or not these short term increases in habitat result in long term benefits to the fish populations has yet to be determined.

  13. Project 8, Phase III Design: Placing an eV-Scale Limit on the Neutrino Mass using Cyclotron Radiation Emission Spectroscopy

    NASA Astrophysics Data System (ADS)

    Oblath, Noah; Project 8 Collaboration

    2016-09-01

    We report on the design concept for Phase III of the Project 8 experiment. In the third phase of Project 8 we aim to place a limit on the neutrino mass that is similar to the current limits set by tritium beta-decay experiments, mν < 2eV . From the first two phases of Project 8 we move to a novel design consisting of a 100cm3 cylindrical volume of tritium gas instrumented with two 30-element rings of inward-facing antennas. Beam-forming techniques similar to those used in radioastronomy will be employed to search for and track electron signals in the fiducial volume. This talk will present the quantitative design concept for the phased-array receiver, and illustrate how we are progressing towards the Phase IV experiment, which will have sensitivity to the neutrino mass scale allowed by the inverted mass hierarchy. This work is supported by the DOE Office of Science Early Career Research Program, and the Laboratory Directed Research and Development Program at Pacific Northwest National Laboratory.

  14. Catalytic activity of ruthenium(III) on the oxidation of an anticholinergic drug-atropine sulfate monohydrate by copper(III) periodate complex in aqueous alkaline medium - decarboxylation and free radical mechanism.

    PubMed

    Byadagi, Kirthi S; Nandibewoor, Sharanappa T; Chimatadar, Shivamurti A

    2013-01-01

    Atropine sulfate monohydrate (ASM) is an anticholinergic drug, having a wide spectrum of activity. Hence, the kinetics of oxidation of ASM by diperiodatocuperate (DPC) in the presence of micro (10-6) amounts of Ru(III) catalyst has been investigated spectrophotometrically in aqueous alkaline medium at I = 0.50 mol dm-3. The reaction between DPC and ASM exhibits 1:2 stoichiometry (ASM:DPC) i. e., one mole of ASM require two moles of DPC to give products. The main oxidation products were confirmed by spectral studies. The reaction is first order with respect to [DPC] and [Ru(III)], while the order with respect to [ASM] and [OH-] was less than unity. The rates decreased with increase in periodate concentration. The reaction rates revealed that Ru(III) catalyzed reaction was about seven-fold faster than the uncatalyzed reaction. The catalytic constant (KC) was also determined at different temperatures. A plausible mechanism is proposed. The activation parameters with respect to slow step of the mechanism were calculated and the thermodynamic quantities were also determined. Kinetic experiments suggest that [Cu(H2IO6)(H2O)2] is the reactive Cu(III) species and [Ru(H2O)5OH]2+ is the reactive Ru(III) species.

  15. An Excerpt From Evaluation of Title III Programs in the Detroit Public Schools (A Report of the Third Years' Activities). Part One.

    ERIC Educational Resources Information Center

    Rosenfeld, Michael; Wilkes, Ronald

    The specific focus of this document is on the evolutionary development of the Neighborhood Educational Center Project supported under Title III, 1965 Elementary Secondary Act, which was marked by continual evaluation and appropriate modification. Application of the concepts of individualized instruction was one of the basic means by which the…

  16. Energy and Safety: Science Activities for Elementary Students, Level III (Grades (5-6).

    ERIC Educational Resources Information Center

    Westcott, Dale; And Others

    Thirteen activities are presented that focus on a common phenomenon of a child's world: energy. These activities relate energy, how it occurs, how it is used, and how to use it safely. Each activity includes the purpose, introduction, background, procedure, materials, estimated time for the activity, typical results, safety notes, and more ideas.…

  17. Mitochondrial Complex I Activity is Conditioned by Supercomplex I-III2-IV Assembly in Brain Cells: Relevance for Parkinson's Disease.

    PubMed

    Lopez-Fabuel, Irene; Resch-Beusher, Monica; Carabias-Carrasco, Monica; Almeida, Angeles; Bolaños, Juan P

    2017-02-14

    The assembly of complex I (CI) with complexes III (CIII) and IV (CIV) of the mitochondrial respiratory chain (MRC) to configure I-III- or I-III-IV-containing supercomplexes (SCs) regulates mitochondrial energy efficiency and reactive oxygen species (mROS) production. However, whether the occurrence of SCs impacts on CI specific activity remains unknown to our knowledge. To investigate this issue, here we determined CI activity in primary neurons and astrocytes, cultured under identical antioxidants-free medium, from two mouse strains (C57Bl/6 and CBA) and Wistar rat, i.e. three rodent species with or without the ability to assemble CIV into SCs. We found that CI activity was 6- or 1.8-fold higher in astrocytes than in neurons, respectively, from rat or CBA mouse, which can form I-III2-IV SC; however, CI activity was similar in the cells from C57Bl/6 mouse, which does not form I-III2-IV SC. Interestingly, CII-III activity, which was comparable in neurons and astrocytes from mice, was about 50% lower in astrocytes when compared with neurons from rat, a difference that was abolished by antioxidants- or serum-containing media. CIV and citrate synthase activities were similar under all conditions studied. Interestingly, in rat astrocytes, CI abundance in I-III2-IV SC was negligible when compared with its abundance in I-III-containing SCs. Thus, CIV-containing SCs formation may determine CI specific activity in astrocytes, which is important to understand the mechanism for CI deficiency observed in Parkinson's disease.

  18. Clean Coal Technology III: 10 MW Demonstration of Gas Suspension Absorption final project performance and economics report

    SciTech Connect

    Hsu, F.E.

    1995-08-01

    The 10 MW Demonstration of the Gas Suspension Absorption (GSA) program is a government and industry co-funded technology development. The objective of the project is to demonstrate the performance of the GSA system in treating a 10 MW slipstream of flue gas resulting from the combustion of a high sulfur coal. This project involves design, fabrication, construction and testing of the GSA system. The Project Performance and Economics Report provides the nonproprietary information for the ``10 MW Demonstration of the Gas Suspension Absorption (GSA) Project`` installed at Tennessee Valley Authority`s (TVA) Shawnee Power Station, Center for Emissions Research (CER) at Paducah, Kentucky. The program demonstrated that the GSA flue-gas-desulfurization (FGD) technology is capable of achieving high SO{sub 2} removal efficiencies (greater than 90%), while maintaining particulate emissions below the New Source Performance Standards (NSPS), without any negative environmental impact (section 6). A 28-day test demonstrated the reliability and operability of the GSA system during continuous operation. The test results and detailed discussions of the test data can be obtained from TVA`s Final Report (Appendix A). The Air Toxics Report (Appendix B), prepared by Energy and Environmental Research Corporation (EERC) characterizes air toxic emissions of selected hazardous air pollutants (HAP) from the GSA process. The results of this testing show that the GSA system can substantially reduce the emission of these HAP. With its lower capital costs and maintenance costs (section 7), as compared to conventional semi-dry scrubbers, the GSA technology commands a high potential for further commercialization in the United States. For detailed information refer to The Economic Evaluation Report (Appendix C) prepared by Raytheon Engineers and Constructors.

  19. Community Based Education Activities in Southeastern Region Teacher Corps Projects.

    ERIC Educational Resources Information Center

    White, Martha; Bonney, Nancy

    To gather information on the community councils mandated for each Teacher Corps project, this pilot study surveyed 34 project directors and council chairpersons in 24 southeastern projects initiated in 1978 and 1979. The study's questionnaires asked about community council elections, membership, training, leadership, meetings, procedures, interest…

  20. Biological effects of high-LET particles on corn-seed embryos in the Apollo-Soyuz Test Project--Biostack III experiment.

    PubMed

    Peterson, D D; Benton, E V; Tran, M; Yang, T; Freeling, M; Craise, L; Tobias, C A

    1977-01-01

    High-LET particle hits in embryos of Zea mays corn seeds, flown as part of Biostack III in the Apollo-Soyuz Test Project, were determined via plastic nuclear track detectors. Based on etched particle-track measurements, 41 embryos were hit in seed layer 1 which contained 80 seeds, and 49 hits occurred in layer 2 which contained 79 seeds. The mean LET value and range of atomic numbers of recorded hits is, respectively, 210 +/- 57 keV micrometers -1 and 9 < or approximately Z < or approximately 26. Detailed analysis of one particular seed showing marked growth anomalies revealed two hits in the central region of the embryo. These two hits had LET values in the region of 100-150 keV micrometers-1, and Z > or approximately 20.

  1. The efficacy of recombinant human activated protein C (rhAPC) vs antithrombin III (at III) vs heparin, in the healing process of partial-thickness burns: a comparative study

    PubMed Central

    Kritikos, O.; Tsagarakis, M.; Tsoutsos, D.; Kittas, C.; Gorgoulis, V.; Papalois, A.; Giannopoulos, A.; Kakiopoulos, G.; Papadopoulos, O.

    2012-01-01

    Summary This is an experimental study regarding the positive effect of recombinant human activated protein C (rhAPC) in the healing process of partial-thickness burns, in comparison to antithrombin III and heparin. On a porcine model we induced superficial partial-thickness and deep partial-thickness burns and performed intravenous administration of the elements of study during the first 48 h. The progress of the condition of the injured tissues was evaluated by histopathological examination at specific time intervals. The results showed an improved healing response of the specimens treated with rhAPC compared to those treated with antithrombin III, heparin, and placebo. PMID:23233823

  2. Mechanism for adhesion G protein-coupled receptor GPR56-mediated RhoA activation induced by collagen III stimulation.

    PubMed

    Luo, Rong; Jeong, Sung-Jin; Yang, Annie; Wen, Miaoyun; Saslowsky, David E; Lencer, Wayne I; Araç, Demet; Piao, Xianhua

    2014-01-01

    GPR56 is a member of the adhesion G protein-coupled receptor (GPCR) family. Despite the importance of GPR56 in brain development, where mutations cause a devastating human brain malformation called bilateral frontoparietal polymicrogyria (BFPP), the signaling mechanism(s) remain largely unknown. Like many other adhesion GPCRs, GPR56 is cleaved via a GPCR autoproteolysis-inducing (GAIN) domain into N- and C-terminal fragments (GPR56N and GPR56C); however, the biological significance of this cleavage is elusive. Taking advantage of the recent identification of a GPR56 ligand and the presence of BFPP-associated mutations, we investigated the molecular mechanism of GPR56 signaling. We demonstrate that ligand binding releases GPR56N from the membrane-bound GPR56C and triggers the association of GPR56C with lipid rafts and RhoA activation. Furthermore, one of the BFPP-associated mutations, L640R, does not affect collagen III-induced lipid raft association of GPR56. Instead, it specifically abolishes collagen III-mediated RhoA activation. Together, these findings reveal a novel signaling mechanism that may apply to other members of the adhesion GPCR family.

  3. Synthesis, molecular structure, theoretical calculation, DNA/protein interaction and cytotoxic activity of manganese(III) complex with 8-hydroxyquinoline.

    PubMed

    Thamilarasan, V; Sengottuvelan, N; Sudha, A; Srinivasan, P; Siva, A

    2015-01-01

    Manganese(III) complex (1) [Mn(8-hq)3] (where 8-hq=8-hydroxyquinoline) has been synthesized and characterized by elemental, spectral (UV-vis, FT-IR) and thermal analysis. The structure of complex (1) has been determined by single crystal X-ray diffraction studies and the configuration around manganese(III) ion was elongated octahedral coordination geometry. Density functional theory calculations were performed for ligand and its complex. Binding studies of ligand and complex 1 with calf thymus DNA (CT-DNA) was investigated by absorption, fluorescence, circular dichroic (CD) spectroscopy and viscosity measurements. Absorption spectral studies revealed that ligand and complex 1 binds to DNA groove and its intrinsic binding strength has been found to be 2.57×10(4) and 2.91×10(4)M(-1). A molecular docking study confirm that the complex 1 is a minor groove binder and was stabilized through hydrogen bonding interactions. Complex 1 exhibits a good binding propensity to bovine serum albumin (BSA) protein. The in vitro cytotoxicity study of complex 1 on breast cancer cell line (MCF-7) indicate that it has the potential to act as effective anticancer drug, with IC50 values of 3.25μM. The ligand and its complex have been screened for antimicrobial activities and the complex showed better antimicrobial activity than the free ligand.

  4. Active Control of Low-Speed Fan Tonal Noise Using Actuators Mounted in Stator Vanes: Part III Results

    NASA Technical Reports Server (NTRS)

    Sutliff, Daniel L.; Remington, Paul J.; Walker, Bruce E.

    2003-01-01

    A test program to demonstrate simplification of Active Noise Control (ANC) systems relative to standard techniques was performed on the NASA Glenn Active Noise Control Fan from May through September 2001. The target mode was the m = 2 circumferential mode generated by the rotor-stator interaction at 2BPF. Seven radials (combined inlet and exhaust) were present at this condition. Several different error-sensing strategies were implemented. Integration of the error-sensors with passive treatment was investigated. These were: (i) an in-duct linear axial array, (ii) an induct steering array, (iii) a pylon-mounted array, and (iv) a near-field boom array. The effect of incorporating passive treatment was investigated as well as reducing the actuator count. These simplified systems were compared to a fully ANC specified system. Modal data acquired using the Rotating Rake are presented for a range of corrected fan rpm. Simplified control has been demonstrated to be possible but requires a well-known and dominant mode signature. The documented results here in are part III of a three-part series of reports with the same base title. Part I and II document the control system and error-sensing design and implementation.

  5. Activation of Manganese Oxidants with Bisulfite for Enhanced Oxidation of Organic Contaminants: The Involvement of Mn(III).

    PubMed

    Sun, Bo; Guan, Xiaohong; Fang, Jingyun; Tratnyek, Paul G

    2015-10-20

    MnO4(-) was activated by HSO3(-), resulting in a process that oxidizes organic contaminants at extraordinarily high rates. The permanganate/bisulfite (PM/BS) process oxidized phenol, ciprofloxacin, and methyl blue at pHini 5.0 with rates (kobs ≈ 60-150 s(-1)) that were 5-6 orders of magnitude faster than those measured for permanganate alone, and ∼5 to 7 orders of magnitude faster than conventional advanced oxidation processes for water treatment. Oxidation of phenol was fastest at pH 4.0, but still effective at pH 7.0, and only slightly slower when performed in tap water. A smaller, but still considerable (∼3 orders of magnitude) increase in oxidation rates of methyl blue was observed with MnO2 activated by HSO3(-) (MO/BS). The above results, time-resolved spectroscopy of manganese species under various conditions, stoichiometric analysis of pH changes, and the effect of pyrophosphate on UV absorbance spectra suggest that the reactive intermediate(s) responsible for the extremely rapid oxidation of organic contaminants in the PM/BS process involve manganese(III) species with minimal stabilization by complexation. The PM/BS process may lead to a new category of advanced oxidation technologies based on contaminant oxidation by reactive manganese(III) species, rather than hydroxyl and sulfate radicals.

  6. Sonochemical synthesis, characterization, antimicrobial activity and textile dyeing behavior of nano-sized cobalt(III) complexes.

    PubMed

    Bala, Ritu; Behal, Jagriti; Kaur, Varinder; Jain, Subheet Kumar; Rani, Riveka; Manhas, Rajesh Kumari; Prakash, Vinit

    2017-03-01

    Using ultrasonic irradiations, nano-sized cobalt(III) coordination complexes, [Co(NH3)6]Cl3·2H2O (A), [Co(en)3]Cl3·3H2O (B) (en-ethylenediamine) and [Co(dien)2]Cl3·3.5H2O (C) (dien-diethylenetriamine) were synthesized. These complexes were characterized by spectroscopic studies like IR, UV/Visible and NMR. Morphology of these complexes was determined by SEM and particle size with the help of TEM & Zeta-sizer. The comparative thermal stability along with phase difference between nano structures and their respective bulk complexes has been studied by thermal gravimetric analysis (TGA) and X-ray powder diffraction (XRD) study respectively. The dyeing behavior of nano-sized Co(III) complexes and their respective bulks has also been studied (using both exhaust and pad dyeing methods) on cotton and wool fabrics and results shown rationalized dyeing behavior. All these complexes were further tested for antimicrobial activity (against B. subtilis, E. coli, K. pneumoniae, F. oxysporum and A. alternate) and it was observed that nano sized complexes enhanced the activity further.

  7. The role of hyperglycaemia-induced alterations of antithrombin III and factor X activation in the thrombin hyperactivity of diabetes mellitus.

    PubMed

    Ceriello, A; Quatraro, A; Marchi, E; Barbanti, M; Dello Russo, P; Lefebvre, P; Giugliano, D

    1990-05-01

    Factor X concentration and factor X activation, antithrombin III anti-Xa activity and plasma concentration, and fibrinopeptide A were measured in 20 diabetic patients and 20 normal subjects. Although factor X activation (81.3 +/- 2.2 vs 97.3 +/- 2.1%, p less than 0.01; mean +/- SE) and antithrombin III activity (76.5 +/- 2.2 vs 96.3 +/- 1.8%, p less than 0.01) were reduced in the diabetic patients, fibrinopeptide A concentration was increased (3.7 +/- 0.4 vs 1.7 +/- 0.2 ng ml-1, p less than 0.01). The ratio of factor X activation to antithrombin III anti-factor Xa activity was increased in the diabetic patients (1.10 +/- 0.01 vs 1.01 +/- 0.02, p less than 0.01). Induced hyperglycaemia was able to mimic all these abnormalities, without changing factor X or antithrombin III concentration. The results suggest that in vivo hyperglycaemia produces a decrease of factor X activation, but at the same time increases fibrinopeptide A formation due to a greater decrease of antithrombin III anti-Xa activity.

  8. The Mark III VLBI System

    NASA Technical Reports Server (NTRS)

    Rogers, A. E. E.; Whitney, A. R.; Levine, J. I.; Nesman, E. F.; Webber, J. C.; Hinteregger, H. F.

    1988-01-01

    Geodetic measurements have errors in centimeter range. Collection of three reports describes both equipment and results of some measurements taken with Mark III very-long-baseline interferometry (VLBI) system. Has demonstrated high accuracy over short baselines, where phase-delay measurements used. Advanced hardware, called Mark III A, developed to improve system performance and efficiency. Original Mark III hardware and III A subsystem upgrades developed as part of NASA Crustal Dynamics Project at Haystack Observatory.

  9. Project Real World: Economic Living Skills for High School Students. Module III, Resource Management Skills--What Money Can't Buy.

    ERIC Educational Resources Information Center

    Federal/Provincial Consumer Education and Plain Language Task Force (Canada).

    Project Real World, a self-contained, activity-based Canadian consumer science program, provides students with systematic instruction in economic living skills. It gives students in grades 10-12 an orientation to the economic realities and opportunities in society. The program helps students function effectively within the rapidly changing…

  10. Identification of a recombinant inulin fructotransferase (difructose dianhydride III forming) from Arthrobacter sp. 161MFSha2.1 with high specific activity and remarkable thermostability.

    PubMed

    Wang, Xiao; Yu, Shuhuai; Zhang, Tao; Jiang, Bo; Mu, Wanmeng

    2015-04-08

    Difructose dianhydride III (DFA III) is a functional carbohydrate produced from inulin by inulin fructotransferase (IFTase, EC 4.2.2.18). In this work, an IFTase gene from Arthrobacter sp. 161MFSha2.1 was cloned and expressed in Escherachia coli. The recombinant enzyme was purified by metal affinity chromatography. It showed significant inulin hydrolysis activity, and the produced main product from inulin was determined as DFA III by nuclear magnetic resonance analysis. The molecular mass of the purified protein was calculated to be 43 and 125 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration, respectively, suggesting the native enzyme might be a homotrimer. The recombinant enzyme showed maximal activity as 2391 units/mg at pH 6.5 and 55 °C. It displayed the highest thermostability among previously reported IFTases (DFA III forming) and was stable up to 80 °C for 4 h of incubation. The smallest substrate was determined as nystose. The conversion ratio of inulin to DFA III reached 81% when 100 g/L inulin was catalyzed by 80 nM recombinant enzyme for 20 min at pH 6.5 and 55 °C. All of these data indicated that the IFTase (DFA III forming) from Arthrobacter sp. 161MFSha2.1 had great potential for industrial DFA III production.

  11. Assessing braze quality in the actively cooled Tore Supra Phase III outboard pump limiter

    SciTech Connect

    Nygren, R.E.; Lutz, T.L.; Miller, J.D.; McGrath, R.; Dale, G.

    1994-12-31

    The quality of brazing of pyrolytic graphite armor brazed to copper tubes in Tore Supra`s Phase III Outboard Pump Limiter was assessed through pre-service qualification testing of individual copper/tile assemblies. The evaluation used non-destructive, hot water transient heating tests performed in the high-temperature, high-pressure flow loop at Sandia`s Plasma Materials Test Facility. Surface temperatures of tiles were monitored with an infrared camera as water at 120{degrees}C at about 2.07 MPa (300 psi) passed through a tube assembly initially at 30{degrees}C. For tiles with braze voids or cracks, the surface temperatures tagged behind those of adjacent well-bonded tiles. Temperature tags were correlated with flaw sizes observed during repairs based upon a detailed 2-D heat transfer analyses. {open_quotes}Bad{close_quotes} tiles, i.e., temperature tags of 10-20{degrees}C depending upon tile`s size, were easy to detect and, when removed, revealed braze voids of roughly 50% of the joint area. Eleven of the 14 tubes were rebrazed after bad tiles were detected and removed. Three tubes were rebrazed twice.

  12. TORC1-dependent sumoylation of Rpc82 promotes RNA polymerase III assembly and activity

    PubMed Central

    Chymkowitch, Pierre; Nguéa P, Aurélie; Aanes, Håvard; Robertson, Joseph; Klungland, Arne; Enserink, Jorrit M.

    2017-01-01

    Maintaining cellular homeostasis under changing nutrient conditions is essential for the growth and development of all organisms. The mechanisms that maintain homeostasis upon loss of nutrient supply are not well understood. By mapping the SUMO proteome in Saccharomyces cerevisiae, we discovered a specific set of differentially sumoylated proteins mainly involved in transcription. RNA polymerase III (RNAPIII) components, including Rpc53, Rpc82, and Ret1, are particularly prominent nutrient-dependent SUMO targets. Nitrogen starvation, as well as direct inhibition of the master nutrient response regulator target of rapamycin complex 1 (TORC1), results in rapid desumoylation of these proteins, which is reflected by loss of SUMO at tRNA genes. TORC1-dependent sumoylation of Rpc82 in particular is required for robust tRNA transcription. Mechanistically, sumoylation of Rpc82 is important for assembly of the RNAPIII holoenzyme and recruitment of Rpc82 to tRNA genes. In conclusion, our data show that TORC1-dependent sumoylation of Rpc82 bolsters the transcriptional capacity of RNAPIII under optimal growth conditions. PMID:28096404

  13. Substrate-Activated Conformational Switch on Chaperones Encodes aTargeting Signal in Type III Secretion

    PubMed Central

    Chen, Li; Ai, Xuanjun; Portaliou, Athina G.; Minetti, Conceicao A.S.A.; Remeta, David P.; Economou, Anastassios; Kalodimos, Charalampos G.

    2013-01-01

    SUMMARY Targeting of type III secretion proteins at the injectisome is an important process in bacterial virulence. Nevertheless, how the injectisome specifically recognizes TTS substrates among all bacterial proteins is unknown. A TTS peripheral membrane ATPase protein located at the base of the injectisome has been implicated in the targeting process. We have investigated the targeting of the EspA filament protein and its cognate chaperone CesAB to the EscN ATPase of the enteropathogenic E. coli (EPEC). We show that EscN selectively engages the EspA-loaded CesAB, but not the unliganded CesAB. Structure analysis revealed that the targeting signal is encoded in a disorder-order structural transition in CesAB that is elicited only upon binding of its physiological substrate, EspA. Abrogation of the interaction between the CesAB–EspA complex and EscN resulted in severe secretion and infection defects. We further show that the targeting and secretion signals are distinct and the two processes are likely regulated by different mechanisms. PMID:23523349

  14. Synthesis, characterization, antioxidant activity and DNA-binding studies of three rare earth (III) complexes with 1-(4-aminoantipyrine)-3-tosylurea ligand.

    PubMed

    Xi, Pin-xian; Xu, Zhi-hong; Liu, Xiao-hui; Chen, Feng-juan; Zeng, Zheng-zhi; Zhang, Xiao-wen; Liu, Ying

    2009-01-01

    1-(4-aminoantipyrine)-3-tosylurea (H2L) and its three lanthanide (III) complexes, M(H2L)3 3NO3 [where M=Nd(III), Sm(III) and Eu(III)], have been synthesized and characterized. In addition, the DNA-binding properties of the three complexes have been investigated by UV-vis (ultraviolet and visible) absorption spectroscopy, fluorescence spectroscopy, circular dichroism (CD) spectroscopy, cyclic voltammetry, and viscosity measurements. Results suggest that the three complexes bind to DNA via a groove binding mode. Furthermore, the antioxidant activity (superoxide and hydroxyl radical) of the metal complexes was determined by using spectrophotometer methods in vitro. These complexes were found to possess potent antioxidant activity and be better than standard antioxidants like vitamin C and mannitol.

  15. Erratum: The Extragalactic Distance Scale Key Project. III. The Discovery of Cepheids and a New Distance to M101 Using the Hubble Space Telescope

    NASA Astrophysics Data System (ADS)

    Kelson, Daniel D; Illingworth, Garth D.; Freedman, Wendy F.; Graham, John A.; Hill, Robert; Madore, Barry F.; Saha, Abhijit; Stetson, Peter B.; Kennicutt, Robert C., Jr.; Mould, Jeremy R.; Hughes, Shaun M.; Ferrarese, Laura; Phelps, Randy; Turner, Anne; Cook, Kem H.; Ford, Holland; Hoessel, John G.; Huchra, John

    1997-03-01

    In the paper ``The Extragalactic Distance Scale Key Project. III. The Discovery of Cepheids and a New Distance to M101 Using the Hubble Space Telescope'' by Daniel D. Kelson, Garth D. Illingworth, Wendy F. Freedman, John A. Graham, Robert Hill, Barry F. Madore, Abhijit Saha, Peter B. Stetson, Robert C. Kennicutt, Jr., Jeremy R. Mould, Shaun M. Hughes, Laura Ferrarese, Randy Phelps, Anne Turner, Kem H. Cook, Holland Ford, John G. Hoessel, and John Huchra (ApJ, 463, 26 [1996]), two of the tables are in error. The magnitudes in Tables B1 and B2, in Appendix B, are ordered incorrectly. As a result, the Julian dates are not associated with their correct Cepheid magnitudes. We have now corrected these data, and updated versions of the tables are available on the World Wide Web. The tables are available in ASCII format at our Key Project site (http://www.ipac.caltech.edu/H0kp/) and will appear in volume 7 of the AAS CDROM. PostScript and paper copies are also available from the first author (http://www.ucolick.org/~kelson/H0/home.html or kelson@ucolick.org).

  16. Learning Activity Packets for Grinding Machines. Unit III--Cylindrical Grinding.

    ERIC Educational Resources Information Center

    Oklahoma State Board of Vocational and Technical Education, Stillwater. Curriculum and Instructional Materials Center.

    This learning activity packet (LAP) is one of three that accompany the curriculum guide on grinding machines. It outlines the study activities and performance tasks for the third unit of this curriculum guide. Its purpose is to aid the student in attaining a working knowledge of this area of training and in achieving a skilled or moderately…

  17. Learning Activity Packets for Milling Machines. Unit III--Vertical Milling Machines.

    ERIC Educational Resources Information Center

    Oklahoma State Board of Vocational and Technical Education, Stillwater. Curriculum and Instructional Materials Center.

    This learning activity packet (LAP) outlines the study activities and performance tasks covered in a related curriculum guide on milling machines. The course of study in this LAP is intended to help students learn to set up and operate a vertical mill. Tasks addressed in the LAP include mounting and removing cutters and cutter holders for vertical…

  18. The Hubble Space Telescope quasar absorption line key project. III - First observational results on Milky Way gas

    NASA Technical Reports Server (NTRS)

    Savage, Blair D.; Lu, Limin; Bahcall, John N.; Bergeron, Jacqueline; Boksenberg, Alec; Hartig, George F.; Jannuzi, Buell T.; Kirhakos, Sofia; Lockman, Felix J.; Sargent, W. L. W.

    1993-01-01

    Absorption lines found near zero redshift due to Milky Way disk and halo gas in the spectra of 15 quasars observed with the Faint Object Spectrograph (FOS) of the HST at a resolution of about 230 km/s are reported. Results show that Milky Way absorption lines comprise about 44 percent of all absorption lines seen in the first group of Key Project FOS spectra. Milky Way lines were observed for 3C 273 and H1821 + 643. Limits to the Mg-to-H abundance ratio obtained for very high velocity Mg II absorption detections imply gas-phase Mg abundances for the very high velocity gas ranging from more than 0.059 to more than 0.32 times the solar abundance. In all cases where high-velocity H I emission is seen, corresponding high-velocity metal-line absorption is observed.

  19. Rift Valley fever dynamics in Senegal: a project for pro-active adaptation and improvement of livestock raising management.

    PubMed

    Lafaye, Murielle; Sall, Baba; Ndiaye, Youssou; Vignolles, Cecile; Tourre, Yves M; Borchi, Franc Ois; Soubeyroux, Jean-Michel; Diallo, Mawlouth; Dia, Ibrahima; Ba, Yamar; Faye, Abdoulaye; Ba, Taibou; Ka, Alioune; Ndione, Jacques-André; Gauthier, Hélène; Lacaux, Jean-Pierre

    2013-11-01

    The multi-disciplinary French project "Adaptation à la Fiévre de la Vallée du Rift" (AdaptFVR) has concluded a 10-year constructive interaction between many scientists/partners involved with the Rift Valley fever (RVF) dynamics in Senegal. The three targeted objectives reached were (i) to produce--in near real-time--validated risk maps for parked livestock exposed to RVF mosquitoes/vectors bites; (ii) to assess the impacts on RVF vectors from climate variability at different time-scales including climate change; and (iii) to isolate processes improving local livestock management and animal health. Based on these results, concrete, pro-active adaptive actions were taken on site, which led to the establishment of a RVF early warning system (RVFews). Bulletins were released in a timely fashion during the project, tested and validated in close collaboration with the local populations, i.e. the primary users. Among the strategic, adaptive methods developed, conducted and evaluated in terms of cost/benefit analyses are the larvicide campaigns and the coupled bio-mathematical (hydrological and entomological) model technologies, which are being transferred to the staff of the "Centre de Suivi Ecologique" (CSE) in Dakar during 2013. Based on the results from the AdaptFVR project, other projects with similar conceptual and modelling approaches are currently being implemented, e.g. for urban and rural malaria and dengue in the French Antilles.

  20. Inferring Earthquake Physics from Deep Drilling Projects of Active Faults

    NASA Astrophysics Data System (ADS)

    Di Toro, G.; Smith, S. A. F.; Kuo, L. W.; Mittempergher, S.; Remitti, F.; Spagnuolo, E.; Mitchell, T. M.; Gualtieri, A.; Hadizadeh, J.; Carpenter, B. M.

    2014-12-01

    Deep drilling projects of active faults offer the opportunity to correlate physical and chemical processes identified in core samples with experiments reproducing the seismic cycle in the laboratory and with high-resolution seismological and geophysical data. Here we discuss the constraints about earthquakes source processes at depth gained by fault cores retrieved from the deep drilling projects SAFOD (2.7 km depth, San Andreas Fault), J-FAST (0.9 km depth, following the Mw 9.0 Tohoku 2011 earthquake), TCDP (1.1 km depth, following the Mw 7.6 Chi-Chi 1999 earthquake) and WFSD (1.2 km depth, following the Mw 7.9 Wenchuan 2008 earthquake). Recovered samples were tested at room temperature with the rotary shear apparatus SHIVA installed in Rome (INGV, Italy). All the tested samples were made by clay-rich gouges (usually including smectite/illite), though their bulk mineralogy and modal composition were different (e.g., SAFOD samples included saponite, WFSD carbonaceous materials). The gouges were investigated before and after the experiments with scanning and transmission electron microscopy, X-Ray diffraction, micro-Raman spectroscopy, etc. A common behavior of all the tested gouges was that their friction coefficient was low (often less than 0.1) under room-humidity and wet conditions when sheared at slip rates of ca. 1 m/s (seismic deformation conditions). Moreover, when the natural fault rocks next to the principal slipping zones were sheared from sub-seismic (few micrometers/s) to seismic slip rates, the experimental products had similar microstructures to those found in the principal slipping zones of the drilled faults. This included the formation of mirror-like surfaces, graphite-rich materials, foliated gouges, nanograins, amorphous materials, etc. In most cases the mechanical data were consistent with several seismological (> 50 m of seismic slip for the fault zone drilled by J-FAST) and geophysical observations (absence of a thermal anomaly in the fault

  1. Mechanism of catalase activity in aqueous solutions of dimanganese(III,IV) ethylenediamine-N,N prime -diacetate

    SciTech Connect

    Rush, J.D.; Maskos, Z. )

    1990-03-07

    Manganous ions, ligated by ethylenediamine-N,N{prime}-diacetate (edda = L) decompose hydrogen peroxide with a rate law {minus}d(H{sub 2}O{sub 2})/dt = k{sub 17}(Mn(edda))(H{sub 2}O{sub 2}) where k{sub 17} = 5.4 M{sup {minus}1} s{sup {minus}1} at pH 7. The reduction of peroxide to water is initiated by the reaction of Mn{sup II}L with a dinuclear Mn{sup III,IV}L{sub 2}. A subsequent fast reaction between the transient product of this reaction and hydrogen peroxide or tert-butyl hydroperoxide effectively oxidizes Mn(II) to Mn(IV) in a concerted step without formation of the hydroxyl radical. The green mixed-valence complex, which is probably a bis({mu}-oxo)-bridged structure, is stable in neutral aqueous solution and exhibits a 16-line ESR signal in frozen solution. The basis of catalase activity is the autocatalytic formation of this complex when hydrogen peroxide is reduced by manganese(II). The catalase cycle is independent of the formation of oxy radicals. Mononuclear Mn{sup III}edda and Mn{sup II}edda react with superoxide radicals, but the decomposition of peroxide is virtually independent of these reactions. In unbuffered solutions, with a moderate excess of hydrogen peroxide, an oscillation in the concentration of the dinuclear complex is detected. 28 refs., 10 figs., 5 tabs.

  2. Gallium(III), cobalt(III) and copper(II) protoporphyrin IX exhibit antimicrobial activity against Porphyromonas gingivalis by reducing planktonic and biofilm growth and invasion of host epithelial cells.

    PubMed

    Olczak, Teresa; Maszczak-Seneczko, Dorota; Smalley, John W; Olczak, Mariusz

    2012-08-01

    Porphyromonas gingivalis acquires heme for growth, and initiation and progression of periodontal diseases. One of its heme acquisition systems consists of the HmuR and HmuY proteins. This study analyzed the antimicrobial activity of non-iron metalloporphyrins against P. gingivalis during planktonic growth, biofilm formation, epithelial cell adhesion and invasion, and employed hmuY, hmuR and hmuY-hmuR mutants to assess the involvement of HmuY and HmuR proteins in the acquisition of metalloporphyrins. Iron(III) mesoporphyrin IX (mesoheme) and iron(III) deuteroporphyrin IX (deuteroheme) supported planktonic growth of P. gingivalis cells, biofilm accumulation, as well as survival, adhesion and invasion of HeLa cells in a way analogous to protoheme. In contrast, cobalt(III), gallium(III) and copper(II) protoporphyrin IX exhibited antimicrobial activity against P. gingivalis, and thus represent potentially useful antibacterial compounds with which to target P. gingivalis. P. gingivalis hmuY, hmuR and hmuY-hmuR mutants showed decreased growth and infection of epithelial cells in the presence of all metalloporphyrins examined. In conclusion, the HmuY protein may not be directly involved in transport of free metalloporphyrins into the bacterial cell, but it may also play a protective role against metalloporphyrin toxicity by binding an excess of these compounds.

  3. Project DAFNE - Drilling Active Faults in Northern Europe

    NASA Astrophysics Data System (ADS)

    Kukkonen, I. T.; Ask, M. S. V.; Olesen, O.

    2012-04-01

    We are currently developing a new ICDP project 'Drillling Active Faults in Northern Europe' (DAFNE) which aims at investigating, via scientific drilling, the tectonic and structural characteristics of postglacial (PG) faults in northern Fennoscandia, including their hydrogeology and associated deep biosphere [1, 2]. During the last stages of the Weichselian glaciation (ca. 9,000 - 15,000 years B.P.), reduced ice load and glacially affected stress field resulted in active faulting in Fennoscandia with fault scarps up to 160 km long and 30 m high. These postglacial (PG) faults are usually SE dipping, SW-NE oriented thrusts, and represent reactivated, pre-existing crustal discontinuities. Postglacial faulting indicates that the glacio-isostatic compensation is not only a gradual viscoelastic phenomenon, but includes also unexpected violent earthquakes, suggestively larger than other known earthquakes in stable continental regions. The research is anticipated to advance science in neotectonics, hydrogeology and deep biosphere studies, and provide important information for nuclear waste and CO2 disposal, petroleum exploration on the Norwegian continental shelf and studies of mineral resources in PG fault areas. We expect that multidisciplinary research applying shallow and deep drilling of postglacial faults would provide significant scientific results through generating new data and models, namely: (1) Understanding PG fault genesis and controls of their locations; (2) Deep structure and depth extent of PG faults; (3) Textural, mineralogical and physical alteration of rocks in the PG faults; (4) State of stress and estimates of paleostress of PG faults; (5) Hydrogeology, hydrochemistry and hydraulic properties of PG faults; (6) Dating of tectonic reactivation(s) and temporal evolution of tectonic systems hosting PG faults; (7) Existence/non-existence of deep biosphere in PG faults; (8) Data useful for planning radioactive waste disposal in crystalline bedrock; (9) Data

  4. Tl(I) and Tl(III) activate both mitochondrial and extrinsic pathways of apoptosis in rat pheochromocytoma (PC12) cells

    SciTech Connect

    Hanzel, Cecilia Eliana; Verstraeten, Sandra Viviana

    2009-04-01

    Thallium (Tl) is a highly toxic metal though yet its mechanisms are poorly understood. Previously, we demonstrated that rat pheochromocytoma (PC12) cells exposure to thallous (Tl(I)) or thallic (Tl(III)) cations leads to mitochondrial damage and reduced cell viability. In the present work we comparatively characterized the possible pathways involved in Tl(I)- and Tl(III)- (10-100 {mu}M) mediated decrease in PC12 cells viability. We observed that these cations do not cause cell necrosis but significantly increased the number of cells with apoptotic features. Both cations lead to Bax oligomerization and caused apoptosis inducing factor (AIF), endonuclease G (Endo G), and cytochrome c release from mitochondria, but they did not activate caspase dependent DNAse (CAD). Tl(I)- and Tl(III)-dependent caspases 9 and 3 activation followed similar kinetics, with maximal effects at 18 h of incubation. In addition, Tl(I) promoted phosphatidylserine (PS) exposure. Tl(III) induced 2- and 18-fold increase in Fas content and caspase 8 activity, respectively. Together, experimental results show that Tl(I) and Tl(III) induce PC12 cells apoptosis, although differential pathways are involved. While Tl(I)-mediated cell apoptosis was mainly associated with mitochondrial damage, Tl(III) showed a mixed effect triggering both the intrinsic and extrinsic pathways of apoptosis. These findings contribute to a better understanding of the mechanisms underlying Tl-induced loss of cell viability in PC12 cells.

  5. College Professors' and Instructors' Attitudes toward International Project Activity

    ERIC Educational Resources Information Center

    Ryzhkova, I. V.

    2010-01-01

    The Bologna process, the most successful European project in the field of higher education, calls for colleges and universities to take joint actions to create a unified European educational space. One possible way to accomplish this task is to implement international scientific research projects. In connection with this, it becomes necessary to…

  6. III. The importance of physical activity and aerobic fitness for cognitive control and memory in children.

    PubMed

    Chaddock-Heyman, Laura; Hillman, Charles H; Cohen, Neal J; Kramer, Arthur F

    2014-12-01

    In this chapter, we review literature that examines the association among physical activity, aerobic fitness, cognition, and the brain in elementary school children (ages 7-10 years). Specifically, physical activity and higher levels of aerobic fitness in children have been found to benefit brain structure, brain function, cognition, and school achievement. For example, higher fit children have larger brain volumes in the basal ganglia and hippocampus, which relate to superior performance on tasks of cognitive control and memory, respectively, when compared to their lower fit peers. Higher fit children also show superior brain function during tasks of cognitive control, better scores on tests of academic achievement, and higher performance on a real-world street crossing task, compared to lower fit and less active children. The cross-sectional findings are strengthened by a few randomized, controlled trials, which demonstrate that children randomly assigned to a physical activity intervention group show greater brain and cognitive benefits compared to a control group. Because these findings suggest that the developing brain is plastic and sensitive to lifestyle factors, we also discuss typical structural and functional brain maturation in children to provide context in which to interpret the effects of physical activity and aerobic fitness on the developing brain. This research is important because children are becoming increasingly sedentary, physically inactive, and unfit. An important goal of this review is to emphasize the importance of physical activity and aerobic fitness for the cognitive and brain health of today's youth.

  7. THE BANANA PROJECT. III. SPIN-ORBIT ALIGNMENT IN THE LONG-PERIOD ECLIPSING BINARY NY CEPHEI

    SciTech Connect

    Albrecht, Simon; Winn, Joshua N.; Carter, Joshua A.; Snellen, Ignas A. G.; De Mooij, Ernst J. W.

    2011-01-10

    Binaries are not always neatly aligned. Previous observations of the DI Her system showed that the spin axes of both stars are highly inclined with respect to one another and the orbital axis. Here, we report on a measurement of the spin-axis orientation of the primary star of the NY Cep system, which is similar to DI Her in many respects: it features two young early-type stars ({approx}6 Myr, B0.5V+B2V), in an eccentric and relatively long-period orbit (e = 0.48, P = 15fd3). The sky projections of the rotation vector and the spin vector are well aligned ({beta}{sub p} = 2{sup 0} {+-} 4{sup 0}), in strong contrast to DI Her. Although no convincing explanation has yet been given for the misalignment of DI Her, our results show that the phenomenon is not universal, and that a successful theory will need to account for the different outcome in the case of NY Cep.

  8. Replacement of the Project Manager Reflected Through Activity Theory and Work-System Theory

    NASA Astrophysics Data System (ADS)

    Vartiainen, Tero; Aramo-Immonen, Heli; Jussila, Jari; Pirhonen, Maritta; Liikamaa, Kirsi

    Replacement of the project manager (RPM) is a known phenomenon in information systems (IS) projects, but scant attention is given to it in the project management or IS literature. Given its critical effects on the project business, the organization, the project team, and the project manager, it should be studied in more depth. We identified factors which make RPM occurrences inherently different and we show that work-system theory and activity theory give comprehensive lenses to advance research on RPM. For the future research on RPM we identified three objectives: experiences on RPM, process model for RPM, and organizational culture's influence on RPM occurrences.

  9. Fibronectin type III5 repeat contains a novel cell adhesion sequence, KLDAPT, which binds activated alpha4beta1 and alpha4beta7 integrins.

    PubMed

    Moyano, J V; Carnemolla, B; Domínguez-Jiménez, C; García-Gila, M; Albar, J P; Sánchez-Aparicio, P; Leprini, A; Querzé, G; Zardi, L; Garcia-Pardo, A

    1997-10-03

    The region of fibronectin encompassing type III repeats 4-6 contains a low affinity heparin binding domain, but its physiological significance is not clear. We have studied whether this domain is able to interact with cells as already shown for other heparin binding domains of fibronectin. A computer search based on homologies with known active sites in fibronectin revealed the sequence KLDAPT located in FN-III5. A synthetic peptide containing this sequence induced lymphoid cell adhesion upon treatment with the activating anti-beta1 monoclonal antibody (mAb) TS2/16 or with Mn2+, indicating that KLDAPT was binding to an integrin. A recombinant fragment containing repeat III5 (FN-III5) also mediated adhesion of TS2/16/Mn2+-treated cells while the FN-III6 fragment did not. Soluble KLDAPT peptide inhibited cell adhesion to FN-III5 as well as to a 38-kDa fibronectin fragment and VCAM-1, two previously known ligands for alpha4beta1 integrin. KLDAPT also competed with the binding of soluble alkaline phosphatase-coupled VCAM-Ig to Mn2+-treated alpha4beta1. Furthermore, mAbs anti-alpha4 and anti-alpha4beta7, but not mAbs to other integrins, inhibited cell adhesion to FN-III5 and KLDAPT. These results therefore establish a cell adhesive function for the FN-III5 repeat and show that KLDAPT is a novel fibronectin ligand for activated alpha4 integrins.

  10. Adaptive organic nanoparticles of a teflon-coated iron (III) porphyrin catalytically activate dioxygen for cyclohexene oxidation.

    PubMed

    Aggarwal, Amit; Singh, Sunaina; Samson, Jacopo; Drain, Charles Michael

    2012-07-26

    Self-organized organic nanoparticles (ONP) are adaptive to the environmental reaction conditions. ONP of fluorous alkyl iron(III) porphyrin catalytically oxidize cyclohexene to the allylic oxidation products. In contrast, the solvated metalloporphyrin yields both allylic oxidation and epoxidation products. The ONP system facilitates a greener reaction because about 89% reaction medium is water, molecular oxygen is used in place of synthetic oxidants, and the ambient reaction conditions used require less energy. The enhanced catalytic activity of these ONP is unexpected because the metalloporphyrins in the nanoaggregates are in the close proximity and the TON should diminish by self-oxidative degradation. The fluorous alkyl chain stabilizes the ONP toward self-oxidative degradation.

  11. Operational Control Procedures for the Activated Sludge Process, Part III-A: Calculation Procedures.

    ERIC Educational Resources Information Center

    West, Alfred W.

    This is the second in a series of documents developed by the National Training and Operational Technology Center describing operational control procedures for the activated sludge process used in wastewater treatment. This document deals exclusively with the calculation procedures, including simplified mixing formulas, aeration tank…

  12. Ergosteroids III. Syntheses and biological activity of seco-steroids related to dehydroepiandrosterone.

    PubMed

    Reich, I L; Lardy, H; Wei, Y; Marwah, P; Kneer, N; Powell, D R; Reich, H J

    1998-10-01

    The unusual activity of some D-ring-seco estrogens led us to prepare several seco steroids related to dehydroepiandrosterone (DHEA) and to test for their ability to mimic thyroid hormone and 7-oxo-DHEA (1) as inducers of thermogenic enzymes in rats' livers. Only one, 3 beta-acetoxy-17a-oxa-androst-5-ene-7,17-dione (17), was capable of inducing both mitochondrial glycerophosphate dehydrogenase and malic enzyme. The closely related 3 beta-hydroxy-17a-oxa-androsta-5,15-diene-7,17-diones (both 14 alpha and 14 beta, 14 and 15) induce the formation of malic enzyme but not of glycerophosphate dehydrogenase. The 3 beta-propionyl ester of the above 14 alpha steroid was not active, presumably because it was not deacylated in vivo. The 16,17 dicarboxylic acid (9) produced by opening the D-ring also induced the formation of malic enzyme but not of glycerophosphate dehydrogenase. 3 beta-Acetoxyandrost-5-ene-7,16,17-trione, an intermediate in the synthesis of D-ring seco compounds enhanced the formation of both enzymes. Twelve other D-ring seco compounds were not active. Seco androstanes oxygenated at position 7 and with expanded A or B rings were not active.

  13. Extending the Diffuse Layer Model of Surface Acidity Behavior: III. Estimating Bound Site Activity Coefficients

    EPA Science Inventory

    Although detailed thermodynamic analyses of the 2-pK diffuse layer surface complexation model generally specify bound site activity coefficients for the purpose of accounting for those non-ideal excess free energies contributing to bound site electrochemical potentials, in applic...

  14. A reassessment of the role of activity in the formation of eye-specific retinogeniculate projections.

    PubMed

    Chalupa, Leo M

    2007-10-01

    In all mammalian species the projections from the two eyes to the dorsal lateral geniculate nucleus of the thalamus terminate in separate layers or territories. This mature projection pattern is refined early in development from an initial state where the inputs of the two eyes are overlapping. Here I discuss the results of studies showing that the formation of segregated eye-specific retinogeniculate projections involves activity-mediated binocular competition. I conclude that while retinal activity undoubtedly is involved in this process, the results of recent studies cast doubt on the prevalent notion that retinal waves of activity play an instructional role in the formation of segregated retinal projections.

  15. Consumer involvement in research projects: the activities of research funders.

    PubMed

    O'Donnell, Máire; Entwistle, Vikki

    2004-08-01

    This paper reports findings from a postal questionnaire survey and in-depth interviews with UK funders of health-related research that explored whether, why and how they promote consumer involvement in research projects. Many UK funders of health-related research are adopting a policy of promoting consumer involvement in research projects. Telephone interviews revealed they have several reasons for doing so, and that they vary in the ways they encourage and support researchers to involve consumers. For some, descriptions of consumer involvement in a research proposal are important for project funding decisions. They recognized a need for flexibility when assessing consumer involvement in different contexts. We suggest that funders should continue to work to clarify what they consider to be the parameters of acceptability in terms of consumer involvement and ensure that 'flexible' criteria are fairly applied. Researchers should be aware of particular funders' views when applying for project funding.

  16. Synthesis, characterization, antioxidant activity and DNA-binding studies of two rare earth(III) complexes with naringenin-2-hydroxy benzoyl hydrazone ligand.

    PubMed

    Li, Tian-Rong; Yang, Zheng-Yin; Wang, Bao-Dui; Qin, Dong-Dong

    2008-08-01

    Two novel rare earth complexes, Y(III) complex (1) and Eu(III) complex (2), with naringenin-2-hydroxy benzoyl hydrazone ligand were synthesized and characterized. The interaction of the two metal complexes and the free ligand with calf thymus DNA (CT DNA) was investigated by electronic absorption spectroscopy, fluorescence spectroscopy and viscosity measurement. All the experimental evidences indicate that these three compounds can strongly bind to CT DNA via an intercalation mechanism. The intrinsic binding constants of the Y(III) complex (1), Eu(III) complex (2) and the free ligand with CT DNA were 2.1 x 10(4), 8.5 x 10(4) and 1.6 x 10(4) M(-1), respectively. Furthermore, the antioxidant activity of the metal complexes was determined by hydroxyl radical scavenging method in vitro.

  17. Kinematics and ionization of extended gas in active galaxies. III. The extranuclear properties of NGC 1068

    SciTech Connect

    Baldwin, J.A.; Wilson, A.S.; Whittle, M.

    1987-08-01

    The kinematic and ionization structure of the extranuclear gas of the Seyfert 2 galaxy NGC 1068 has been investigated using a grid of 28 high- and seven low-dispersion long-slit spectra. The velocity field of most of the gas more than 15 arcsec from the nucleus is well described by a rotating disk with an approximately flat rotation curve. The kinematic line of nodes coincides with the major axis of the outermost continuum light, and the disk inclination derived from the axial ratio of the outer isophotes and the kinematic fit are broadly consistent. The progressive change of the position angle of the major axes of the brighter isophotes are evidently due to a superposition of oval or barlike distortions in the galactic plane. The observed ionization structure of the gas can be understood as a mixture of two disk components projected on top of each other. Much of the ionization pattern in the other disk may result from partial beaming of the ionizing continuum radiation as it escapes from the nucleus. 60 references.

  18. Human T cell activation. III. Induction of an early activation antigen, EA 1 by TPA, mitogens and antigens

    SciTech Connect

    Hara, T.; Jung, L.K.L.; FU, S.M.

    1986-03-01

    With human T cells activated for 12 hours by 12-o-tetradecanoyl phorbol-13-acetate (TPA) as immunogen, an IgG/sub 2a/ monoclonal antibody, mAb Ea 1, has been generated to a 60KD phosphorylated protein with 32KD and 28KD subunits. The antigen, Ea 1, is readily detected on 60% of isolated thymocytes by indirect immunofluorescence. A low level of Ea 1 expression is detectable on 2-6% of blood lymphocytes. Isolated T cells have been induced to express Ea 1 by TPA, mitogens and anitgens. TPA activated T cells express Ea 1 as early as 1 hour after activation. By 4 hours, greater than 95% of the T cells stain with mAb Ea 1. About 50% of the PHA or Con A activated T cells express Ea 1 with a similar kinetics. Ea 1 expression proceeds that of IL-2 receptor in these activation processes. T cells activated by soluble antigens (tetanus toxoid and PPD) and alloantigens in MLR also express Ea 1 after a long incubation. About 20% of the T cells stain for Ea 1 at day 6. Ea 1 expression is not limited to activated T cells. B cells activated by TPA or anti-IgM Ab plus B cell growth factor express Ea 1. The kinetics of Ea 1 expression is slower and the staining is less intense. Repeated attempts to detect Ea 1 on resting and activated monocytes and granulocytes have not been successful. Ea 1 expression is due to de novo synthesis for its induction is blocked by cycloheximide and actinomycin D. Ea 1 is the earliest activation antigen detectable to-date.

  19. [Research on Chinese medicine pairs (III)--Their bio-active components].

    PubMed

    Li, Wei-Xia; Tang, Yu-Ping; Liu, Li; Liu, Pei; Su, Shu-Lan; Qian, Da-Wei; Duan, Jin-Ao

    2013-12-01

    The total effect of Chinese medicine pair (CMP) was not the simply addition of two single herbs, but the interaction of their different components. Therefore, the research on the bio-active components of CMP is the basis of CMP compatibility study, and has important significance for revealing the compatibility effect and action mechanism, and creating traditional Chinese medicine (TCM) new drugs. This paper summed up the latest research progress of CMP on the basis of the bio-active components variation regularity of CMP from chemical solutions and content changes in vitro and the actions of CMP on bodies in vivo, in order to further drive the modern basic and applied research of CMP, and to reveal the scientific essence of CMP compatibility.

  20. American Issues Forum: Active Projects--Summary Report [And] Nationally Circulated Materials.

    ERIC Educational Resources Information Center

    National Endowment for the Humanities (NFAH), Washington, DC.

    These two reports briefly describe the active projects and nationally circulated mateirals associated with the American Issues Forum Bicentennial Programs. The summary report of active projects is designed to show how various national media, national and international organizations, corporations, and state and community groups are actively…

  1. 4-H Horticulture Project Activity Guides. Leader's Guide and Units 1-3.

    ERIC Educational Resources Information Center

    Illinois Univ., Urbana. Cooperative Extension Service.

    This document, concerning the 4-H horticulture project, includes a leader's guide and three youth activity guides. The leader's guide can be used to plan group project meetings that are both fun and educational. Activities can be adapted to various age groups. The leader's guide includes basic information for growing plants indoors and outdoors,…

  2. 78 FR 18617 - Recreational Boating Safety Projects, Programs and Activities Funded Under Provisions of the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-27

    ... SECURITY Coast Guard Recreational Boating Safety Projects, Programs and Activities Funded Under Provisions of the Transportation Equity Act for the 21st Century; Accounting of ACTION: Notice. SUMMARY: In 1999... that a detailed accounting of the projects, programs, and activities funded under the...

  3. Antimicrobial and antioxidant activities of Flammulina velutipes polysacchrides and polysacchride-iron(III) complex.

    PubMed

    Dong, Ya-Ru; Cheng, Shu-Jie; Qi, Guo-Hong; Yang, Zhi-Ping; Yin, Shi-Yu; Chen, Gui-Tang

    2017-04-01

    FVP is polysacchrides obtained from Flammulina velutipes. A polysacchride named FVP2 was isolated from FVP by DEAE cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography. FVP-Fe and FVP2-Fe were synthesized by neutralization of FeCl3 carbohydrate solution. The antibacterial and antifungal activities of FVP, FVP2, FVP-Fe, FVP2-Fe were investigated and their antioxidant effects on hydroxyl, 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion, 2,2'-azobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, reducing power, inhibition of malondialdehyde (MDA) were assessed in vitro. The results suggested that FVP-Fe and FVP2-Fe significantly suppressed the growth of bacteria Staphylococcus aureus, Escherichia coli, and Bacillus subtilis, and have relatively strong antioxidant activity to scavenge superoxide anion radical. In addition, FVP exhibited strong antioxidant activity to eliminate hydroxyl, DPPH, ABTS radicals, had high reducing power and inhibited the MDA production of health mice liver homogenate induced by auto-oxidation and Fe(2+)-H2O2 system.

  4. Genetic Dissection of the Signaling Cascade that Controls Activation of the Shigella Type III Secretion System from the Needle Tip

    PubMed Central

    Murillo, I.; Martinez-Argudo, I.; Blocker, A. J.

    2016-01-01

    Many Gram-negative bacterial pathogens use type III secretion systems (T3SSs) for virulence. The Shigella T3SS consists of a hollow needle, made of MxiH and protruding from the bacterial surface, anchored in both bacterial membranes by multimeric protein rings. Atop the needle lies the tip complex (TC), formed by IpaD and IpaB. Upon physical contact with eukaryotic host cells, T3S is initiated leading to formation of a pore in the eukaryotic cell membrane, which is made of IpaB and IpaC. Through the needle and pore channels, further bacterial proteins are translocated inside the host cell to meditate its invasion. IpaD and the needle are implicated in transduction of the host cell-sensing signal to the T3S apparatus. Furthermore, the sensing-competent TC seems formed of 4 IpaDs topped by 1 IpaB. However, nothing further is known about the activation process. To investigate IpaB’s role during T3SS activation, we isolated secretion-deregulated IpaB mutants using random mutagenesis and a genetic screen. We found ipaB point mutations in leading to defects in secretion activation, which sometimes diminished pore insertion and host cell invasion. We also demonstrated IpaB communicates intramolecularly and intermolecularly with IpaD and MxiH within the TC because mutations affecting these interactions impair signal transduction. PMID:27277624

  5. Control of active nitrogen species used for PA-MBE growth of group III nitrides on Si

    NASA Astrophysics Data System (ADS)

    Ohachi, Tadashi; Yamabe, Nobuhiko; Yamamoto, Yuka; Wada, Motoi; Ariyada, Osamu

    2011-03-01

    A new spiral parallel mesh electrode (PME) is presented to control active nitrogen species in plasma-assisted molecular beam epitaxial (PA-MBE) growth of group III nitrides and their alloys. Direct flux of active nitrogen from radio frequency inductive coupled plasma (rf-ICP) discharge was able to be measured using a mesh electrode for filtering charge particles and electron emission due to the self-ionization of nitrogen atoms on a negatively biased electrode. In situ measurement of direct nitrogen atom fluxes using the spiral PME during PA-MBE growth of GaN and AlN on Si substrates is investigated. A linear rf power dependence of direct flux of active species on atoms such as nitrogen (N+N*), where N and N* were ground and excited atoms, respectively, from a rf-ICP was confirmed by the spiral PME. An indirect flux of nitrogen adsorbed (ADS) atoms (N+N*) during discharge was also monitored by the spiral PME and received influence of the wall surface of the growth chamber. ADS nitrogen atoms are able to be used for nitridation of Si surface to grow a double buffer layer (DBL) AlN/β-Si3N4/Si.

  6. The IACOB project . III. New observational clues to understand macroturbulent broadening in massive O- and B-type stars

    NASA Astrophysics Data System (ADS)

    Simón-Díaz, S.; Godart, M.; Castro, N.; Herrero, A.; Aerts, C.; Puls, J.; Telting, J.; Grassitelli, L.

    2017-01-01

    Context. The term macroturbulent broadening is commonly used to refer to a certain type of non-rotational broadening affecting the spectral line profiles of O- and B-type stars. It has been proposed to be a spectroscopic signature of the presence of stellar oscillations; however, we still lack a definitive confirmation of this hypothesis. Aims: We aim to provide new empirical clues about macroturbulent spectral line broadening in O- and B-type stars to evaluate its physical origin. Methods: We used high-resolution spectra of 430 stars with spectral types in the range O4 - B9 (all luminosity classes) compiled in the framework of the IACOB project. We characterized the line broadening of adequate diagnostic metal lines using a combined Fourier transform and goodness-of-fit technique. We performed a quantitative spectroscopic analysis of the whole sample using automatic tools coupled with a huge grid of fastwind models to determine their effective temperatures and gravities. We also incorporated quantitative information about line asymmetries into our observational description of the characteristics of the line profiles, and performed a comparison of the shape and type of line-profile variability found in a small sample of O stars and B supergiants with still undefined pulsational properties and B main-sequence stars with variable line profiles owing to a well-identified type of stellar oscillations or to the presence of spots in the stellar surface. Results: We present a homogeneous and statistically significant overview of the (single snapshot) line-broadening properties of stars in the whole O and B star domain. We find empirical evidence of the existence of various types of non-rotational broadening agents acting in the realm of massive stars. Even though all these additional sources of line-broadening could be quoted and quantified as a macroturbulent broadening from a practical point of view, their physical origin can be different. Contrarily to the early- to

  7. Class III PI3K-mediated prolonged activation of autophagy plays a critical role in the transition of cardiac hypertrophy to heart failure.

    PubMed

    Yu, Peng; Zhang, Yangyang; Li, Chuanfu; Li, Yuehua; Jiang, Surong; Zhang, Xiaojin; Ding, Zhengnian; Tu, Fei; Wu, Jun; Gao, Xiang; Li, Liu

    2015-07-01

    Pathological cardiac hypertrophy often leads to heart failure. Activation of autophagy has been shown in pathological hypertrophic hearts. Autophagy is regulated positively by Class III phosphoinositide 3-kinase (PI3K). However, it is unknown whether Class III PI3K plays a role in the transition of cardiac hypertrophy to heart failure. To address this question, we employed a previously established cardiac hypertrophy model in heat shock protein 27 transgenic mice which shares common features with several types of human cardiomyopathy. Age-matched wild-type mice served as control. Firstly, a prolonged activation of autophagy, as reflected by autophagosome accumulation, increased LC3 conversion and decreased p62 protein levels, was detected in hypertrophic hearts from adaptive stage to maladaptive stage. Moreover, morphological abnormalities in myofilaments and mitochondria were presented in the areas accumulated with autophagosomes. Secondly, activation of Class III PI3K Vacuolar protein sorting 34 (Vps34), as demonstrated by upregulation of Vps34 expression, increased interaction of Vps34 with Beclin-1, and deceased Bcl-2 expression, was demonstrated in hypertrophic hearts from adaptive stage to maladaptive stage. Finally, administration with Wortmaninn, a widely used autophagy inhibitor by suppressing Class III PI3K activity, significantly decreased autophagy activity, improved morphologies of intracellular apartments, and most importantly, prevented progressive cardiac dysfunction in hypertrophic hearts. Collectively, we demonstrated that Class III PI3K plays a central role in the transition of cardiac hypertrophy to heart failure via a prolonged activation of autophagy in current study. Class III PI3K may serve as a potential target for the treatment and management of maladaptive cardiac hypertrophy.

  8. Balanced activity in basal ganglia projection pathways is critical for contraversive movements

    PubMed Central

    Tecuapetla, Fatuel; Matias, Sara; Dugue, Guillaume P.; Mainen, Zachary F.; Costa, Rui M.

    2014-01-01

    The basal ganglia, and the striatum in particular, have been implicated in the generation of contraversive movements. The striatum projects to downstream basal ganglia nuclei through two main circuits, originating in striatonigral and striatopallidal neurons, and different models postulate that the two pathways can work in opposition or synergistically. Here we show striatonigral and striatopallidal neurons are concurrently active during spontaneous contraversive movements. Furthermore, we show that unilateral optogenetic inhibition of either or both projection pathways disrupts contraversive movements. Consistently, simultaneous activation of both neuron types produces contraversive movements. Still, we also show that imbalanced activity between the pathways can result in opposing movements being driven by each projection pathway. These data show that balanced activity in both striatal projection pathways is critical for the generation of contraversive movements and highlights that imbalanced activity between the two projection pathways can result in opposing motor output. PMID:25002180

  9. Spectroscopic, structure and antimicrobial activity of new Y(III) and Zr(IV) ciprofloxacin

    NASA Astrophysics Data System (ADS)

    Sadeek, Sadeek A.; El-Shwiniy, Walaa H.; Zordok, Wael A.; El-Didamony, Akram M.

    2011-02-01

    The preparation and characterization of the new solid complexes [Y(CIP) 2(H 2O) 2]Cl 3·10H 2O and [ZrO(CIP) 2Cl]Cl·15H 2O formed in the reaction of ciprofloxacin (CIP) with YCl 3 and ZrOCl 2·8H 2O in ethanol and methanol, respectively, at room temperature were reported. The isolated complexes have been characterized with elemental analysis, IR spectroscopy, conductance measurements, UV-vis and 1H NMR spectroscopic methods and thermal analyses. The results support the formation of the complexes and indicate that ciprofloxacin reacts as a bidentate ligand bound to the metal ion through the pyridone oxygen and one carboxylato oxygen. The activation energies, E*; entropies, Δ S*; enthalpies, Δ H*; Gibbs free energies, Δ G*, of the thermal decomposition reactions have been derived from thermogravimetric (TGA) and differential thermogravimetric (DTG) curves, using Coats-Redfern and Horowitz-Metzeger methods. The proposed structure of the two complexes was detected by using the density functional theory (DFT) at the B3LYP/CEP-31G level of theory. The ligand as well as their metal complexes was also evaluated for their antibacterial activity against several bacterial species, such as Staphylococcus aureus ( S. aureus), Escherichia coli ( E. coli) and Pseudomonas aeruginosa ( P. aeruginosa) and antifungal screening was studied against two species ( Penicillium ( P. rotatum) and Trichoderma ( T. sp.)). This study showed that the metal complexes are more antibacterial as compared to free ligand and no antifungal activity observed for ligand and their complexes.

  10. Proteomics for everyday use: activities of the HUPO Brain Proteome Project during the 5th HUPO World Congress.

    PubMed

    Hamacher, Michael; Stephan, Christian; Eisenacher, Martin; van Hall, Andre; Marcus, Katrin; Martens, Lennart; Park, Young Mok; Gutstein, Howard B; Herberg, Friedrich; Meyer, Helmut E

    2007-04-01

    Long Beach hosted this year's annual congress of the Human Proteome Organisation (HUPO). In addition to the numerous sessions, talks and poster presentations organized by HUPO itself, several events were arranged by the HUPO initiatives. The Brain Proteome Project (HUPO BPP) was very active, initiating three pre-congress workshops: (i) the kick-off meeting of the EU-funded ProDaC consortium (Proteomics Data Collection) that is aiming at the bioinformatics Standardization in the proteomics field; (ii) the workshop "Standardization Issues in Proteomics: Perspectives from Vendors" giving an overview about the lessons learned by proteomics industrial partners; (iii) the 6th HUPO BPP Workshop "New Proteomics Approaches for further HUPO BPP Studies" offering new concepts for brain-related proteomics studies.

  11. Project-focused activity and knowledge tracker: a unified data analysis, collaboration, and workflow tool for medicinal chemistry project teams.

    PubMed

    Brodney, Marian D; Brosius, Arthur D; Gregory, Tracy; Heck, Steven D; Klug-McLeod, Jacquelyn L; Poss, Christopher S

    2009-12-01

    Advances in the field of drug discovery have brought an explosion in the quantity of data available to medicinal chemists and other project team members. New strategies and systems are needed to help these scientists to efficiently gather, organize, analyze, annotate, and share data about potential new drug molecules of interest to their project teams. Herein we describe a suite of integrated services and end-user applications that facilitate these activities throughout the medicinal chemistry design cycle. The Automated Data Presentation (ADP) and Virtual Compound Profiler (VCP) processes automate the gathering, organization, and storage of real and virtual molecules, respectively, and associated data. The Project-Focused Activity and Knowledge Tracker (PFAKT) provides a unified data analysis and collaboration environment, enhancing decision-making, improving team communication, and increasing efficiency.

  12. Anticancer Activity of Small Molecule and Nanoparticulate Arsenic(III) Complexes

    PubMed Central

    Swindell, Elden P.; Hankins, Patrick L.; Chen, Haimei; Miodragović, Ðenana U.; O'Halloran, Thomas V.

    2014-01-01

    Starting in ancient China and Greece, arsenic-containing compounds have been used in the treatment of disease for over 3000 years. They were used for a variety of diseases in the 20th century, including parasitic and sexually transmitted illnesses. A resurgence of interest in the therapeutic application of arsenicals has been driven by the discovery that low doses of a 1% aqueous solution of arsenic trioxide (i.e. arsenous acid) leads to complete remission of certain types of leukemia. Since FDA approval of arsenic trioxide (As2O3) for treatment of acute promyelocytic leukemia (APL) in 2000, it has become a front line therapy in this indication. There are currently over 100 active clinical trials involving inorganic arsenic or organoarsenic compounds registered with the FDA for the treatment of cancers. New generations of inorganic and organometallic arsenic compounds with enhanced activity or targeted cytotoxicity are being developed to overcome some of the shortcomings of arsenic therapeutics, namely short plasma half-lives and narrow therapeutic window. PMID:24147771

  13. Half-sandwich rhodium(III) transfer hydrogenation catalysts: Reduction of NAD(+) and pyruvate, and antiproliferative activity.

    PubMed

    Soldevila-Barreda, Joan J; Habtemariam, Abraha; Romero-Canelón, Isolda; Sadler, Peter J

    2015-12-01

    Organometallic complexes have the potential to behave as catalytic drugs. We investigate here Rh(III) complexes of general formula [(Cp(x))Rh(N,N')(Cl)], where N,N' is ethylenediamine (en), 2,2'-bipyridine (bpy), 1,10-phenanthroline (phen) or N-(2-aminoethyl)-4-(trifluoromethyl)benzenesulfonamide (TfEn), and Cp(x) is pentamethylcyclopentadienyl (Cp*), 1-phenyl-2,3,4,5-tetramethylcyclopentadienyl (Cp(xPh)) or 1-biphenyl-2,3,4,5-tetramethyl cyclopentadienyl (Cp(xPhPh)). These complexes can reduce NAD(+) to NADH using formate as a hydride source under biologically-relevant conditions. The catalytic activity decreased in the order of N,N-chelated ligand bpy > phen > en with Cp* as the η(5)-donor. The en complexes (1-3) became more active with extension to the Cp(X) ring, whereas the activity of the phen (7-9) and bpy (4-6) compounds decreased. [Cp*Rh(bpy)Cl](+) (4) showed the highest catalytic activity, with a TOF of 37.4±2h(-1). Fast hydrolysis of the chlorido complexes 1-10 was observed by (1)H NMR (<10min at 310K). The pKa* values for the aqua adducts were determined to be ca. 8-10. Complexes 1-9 also catalysed the reduction of pyruvate to lactate using formate as the hydride donor. The efficiency of the transfer hydrogenation reactions was highly dependent on the nature of the chelating ligand and the Cp(x) ring. Competition reactions between NAD(+) and pyruvate for reduction by formate catalysed by 4 showed a preference for reduction of NAD(+). The antiproliferative activity of complex 3 towards A2780 human ovarian cancer cells increased by up to 50% when administered in combination with non-toxic doses of formate, suggesting that transfer hydrogenation can induce reductive stress in cancer cells.

  14. Electron-transfer reactions of cobalt(III) complexes. 1. The kinetic investigation of the reduction of various surfactant cobalt(III) complexes by iron(II) in surface active ionic liquids.

    PubMed

    Nagaraj, Karuppiah; Senthil Murugan, Krishnan; Thangamuniyandi, Pilavadi; Sakthinathan, Subramanian

    2015-05-15

    The kinetics of outer sphere electron transfer reaction of surfactant cobalt(III) complex ions, cis-[Co(en)2(C12H25NH2)2](3+) (1), cis-[Co(dp)2(C12H25NH2)2](3+) (2), cis-[Co(trien)(C12H25NH2)2](3+) (3), cis-[Co(bpy)2(C12H25NH2)2](3+) (4) and cis-[Co(phen)2(C12H25NH2)2](3+) (5) (en: ethylenediamine, dp: diaminopropane, trien : triethylenetetramine, bpy: 2,2'-bipyridyl, phen: 1,10-phenanthroline and C12H25NH2 : dodecylamine) have been interrogated by Fe(2+) ion in ionic liquid (1-butyl-3-methylimidazoliumbromide) medium at different temperatures (298, 303, 308, 313, 318 and 323K) by the spectrophotometry method under pseudo first order conditions using an excess of the reductant. Experimentally the reactions were found to be of second order and the electron transfer as outer sphere. The second order rate constant for the electron transfer reaction in ionic liquids was found to increase with increase in the concentration of all these surfactant cobalt(III) complexes. Among these complexes (from en to phen ligand), complex containing the phenanthroline ligand rate is higher compared to other complexes. By assuming the outer sphere mechanism, the results have been explained based on the presence of aggregated structures containing cobalt(III) complexes at the surface of ionic liquids formed by the surfactant cobalt(III) complexes in the reaction medium. The activation parameters (enthalpy of activation ΔH(‡) and entropy of activation ΔS(‡)) of the reaction have been calculated which substantiate the kinetics of the reaction.

  15. Interleukin-8 (IL-8) over-production and autocrine cell activation are key factors in monomethylarsonous acid [MMA(III)]-induced malignant transformation of urothelial cells.

    PubMed

    Escudero-Lourdes, C; Wu, T; Camarillo, J M; Gandolfi, A J

    2012-01-01

    The association between chronic human exposure to arsenicals and bladder cancer development is well recognized; however, the underlying molecular mechanisms have not been fully determined. We propose that inflammatory responses can play a pathogenic role in arsenic-related bladder carcinogenesis. In previous studies, it was demonstrated that chronic exposure to 50 nM monomethylarsenous acid [MMA(III)] leads to malignant transformation of an immortalized model of urothelial cells (UROtsa), with only 3 mo of exposure necessary to trigger the transformation-related changes. In the three-month window of exposure, the cells over-expressed pro-inflammatory cytokines (IL-1β, IL-6 and IL-8), consistent with the sustained activation of NFKβ and AP1/c-jun, ERK2, and STAT3. IL-8 was over-expressed within hours after exposure to MMA(III), and sustained over-expression was observed during chronic exposure. In this study, we profiled IL-8 expression in UROtsa cells exposed to 50 nM MMA(III) for 1 to 5 mo. IL-8 expression was increased mainly in cells after 3 mo MMA(III) exposure, and its production was also found increased in tumors derived from these cells after heterotransplantation in SCID mice. UROtsa cells do express both receptors, CXCR1 and CXCR2, suggesting that autocrine cell activation could be important in cell transformation. Supporting this observation and consistent with IL-8 over-expression, CXCR1 internalization was significantly increased after three months of exposure to MMA(III). The expression of MMP-9, cyclin D1, bcl-2, and VGEF was significantly increased in cells exposed to MMA(III) for 3 mo, but these mitogen-activated kinases were significantly decreased after IL-8 gene silencing, together with a decrease in cell proliferation rate and in anchorage-independent colony formation. These results suggest a relevant role of IL-8 in MMA(III)-induced UROtsa cell transformation.

  16. Distinct catalytic activity and in vivo roles of the ExoIII and EndoIV AP endonucleases from Sulfolobus islandicus.

    PubMed

    Yan, Zhou; Huang, Qihong; Ni, Jinfeng; Shen, Yulong

    2016-09-01

    AP endonuclease cleaves the phosphodiester bond 5'- to the AP (apurinic or apyrimidinic) sites and is one of the major enzymes involved in base excision repair. So far, the properties of several archaeal AP endonuclease homologues have been characterized in vitro, but little is known about their functions in vivo. Herein, we report on the biochemical and genetic analysis of two AP endonucleases, SisExoIII and SisEndoIV, from the hyperthermophilic crenarchaeon Sulfolobus islandicus REY15A. Both SisExoIII and SisEndoIV exhibit AP endonuclease activity, but neither of them has 3'-5' exonuclease activity. SisExoIII and SisEndoIV have similar K M values on the substrate containing an AP site, but the latter cleaves the AP substrate at a dramatically higher catalytic rate than the former. Unlike other AP endonucleases identified in archaea, SisExoIII and SisEndoIV do not exhibit any cleavage activity on DNA having oxidative damage (8-oxo-dG) or uracil. Genetic analysis revealed that neither gene is essential for cell viability, and the growth of ∆SiRe_2666 (endoIV), ∆SiRe_0100 (exoIII), and ∆SiRe_0100∆SiRe_2666 is not affected under normal growth conditions. However, ∆SiRe_2666 exhibits higher sensitivity to the alkylating agent methyl methanesulfonate (MMS) than ∆SiRe_0100. Over-expression of SiRe_0100 can partially complement the sensitivity of ∆SiRe_2666 to MMS, suggesting a backup role of SisExoIII in AP site processing in vivo. Intriguingly, over-expression of SisEndoIV renders the strain more sensitive to MMS than the control. Taken together, we conclude that SisEndoIV, but not SisExoIII, is the main AP endonuclease that participates directly in base excision repair in S. islandicus.

  17. The Effect of the Electronic Nature of Spectator Ligands in the C-H Bond Activation of Ethylene by Cr(III) Silicates: An ab initio Study.

    PubMed

    Núñez-Zarur, Francisco; Comas-Vives, Aleix

    2015-01-01

    The Phillips catalyst, chromium oxides supported on silica, is one of the most widely used catalysts for the industrial production of polyethylene (PE). We recently synthesized a well-defined mononuclear Cr(III) silicate as active site model of the Phillips catalyst. The catalytic activity of this well-defined catalyst was similar to the industrial Phillips catalyst. We proposed that C-H bond activation of ethylene over a Cr-O bond initiates polymerization in this Cr(III) catalyst. Our results also showed that the presence of a second ethylene olefin in the coordination sphere of Cr decreases the intrinsic energy barrier of the C-H activation of ethylene. In order to understand the effect of this additional ligand in the C-H activation of ethylene by the Cr(III) catalyst, we evaluated the energetics of this step with different spectator ligands (C2H4, C2F4, N2 and CO) coordinated to the Cr center. The Charge Decomposition Analysis (CDA) of the bonding interactions between the Cr(III) catalyst and the ligands showed that the intrinsic energy barrier for the C-H activation of ethylene decreases with the increasing electron-donor properties of the spectator ligand.

  18. Bis(acridine-9-carboxylate)-nitro-europium(III) dihydrate complex a new apoptotic agent through Flk-1 down regulation, caspase-3 activation and oligonucleosomes DNA fragmentation.

    PubMed

    Azab, Hassan A; Hussein, Belal H M; El-Azab, Mona F; Gomaa, Mohamed; El-Falouji, Abdullah I

    2013-01-01

    New bis(acridine-9-carboxylate)-nitro-europium(III) dihydrate complex was synthesized and characterized. In vivo anti-angiogenic activities of bis(acridine-9-carboxylate)-nitro-europium(III) dihydrate complex against Ehrlich ascites carcinoma (EAC) cells are described. The newly synthesized complex resulted in inhibition of proliferation of EAC cells and ascites formation. The anti-tumor effect was found to be through anti-angiogenic activity as evident by the reduction of microvessel density in EAC solid tumors. The anti-angiogenic effect is mediated through down-regulation of VEGF receptor type-2 (Flk-1). The complex was also found to significantly increase the level of caspase-3 in laboratory animals compared to the acridine ligand and to the control group. This was also consistent with the DNA fragmentation detected by capillary electrophoresis that proved the apoptotic effect of the new complex. Our complex exhibited anti-angiogenic and apoptotic activity in vivo, a thing that makes it a potential effective chemotherapeutic agent. The interaction of calf thymus DNA (ct-DNA) with bis(acridine-9-carboxylate)-nitro-europium(III) dihydrate complex has been investigated using fluorescence technique. A competitive experiment of the europium(III)-acridine complex with ethidium bromide (EB) to bind DNA revealed that interaction between the europium(III)-acridine and DNA was via intercalation. The interaction of the synthesized complex with tyrosine kinases was also studied using molecular docking simulation to further substantiate its mode of action.

  19. The OCCUPAC Project (A Project to Develop Occupational Information Learning Activity Packages for Grades K-9). Final Report.

    ERIC Educational Resources Information Center

    Peterson, Marla; And Others

    During Phase I of a two-phase project, 15 OCCUPACS (learning activity packages) were developed, pilot tested in a university laboratory school, field tested in four public school systems, revised, and submitted to professionals for final review. Intended to provide occupational information to children in Grades K-9 at different levels of…

  20. Synthesis, characterization and anticancer activities of two lanthanide(III) complexes with a nicotinohydrazone ligand

    NASA Astrophysics Data System (ADS)

    Xu, Zhou-Qin; Mao, Xian-Jie; Jia, Lei; Xu, Jun; Zhu, Tao-Feng; Cai, Hong-Xin; Bie, Hong-Yan; Chen, Ru-Hua; Ma, Tie-liang

    2015-12-01

    Two isostructural acylhydrazone based complexes, namely [Ce(penh)2(H2O)4](NO3)3·4H2O (1) and [Sm(penh)2(NO3)2](NO3)·C2H5OH (2) (penh = 2-acetylpyridine nicotinohydrazone), have been obtained and characterized by physico-chemical and spectroscopic methods. The ten-coordinated lanthanide metal ion in each complex is surrounded by two independent tridentate neutral acylhydrazones with two ON2 donor sets. The other four coordination oxygen atoms are from four water molecules and two bidentate nitrate anions for complexes 1 and 2, respectively, thus giving distorted bicapped square antiprism geometry. Both complexes have excellent antitumor activity towards human pancreatic cancer (PATU8988), human colorectal cancer (lovo) and human gastric cancer(SGC7901) cell line. Furthermore, the cell apoptosis of complex 1 is detected by AnnexinV/PI flow cytometry.

  1. Laser-activated remote phosphor light engine for projection applications

    NASA Astrophysics Data System (ADS)

    Daniels, Martin; Mehl, Oliver; Hartwig, Ulrich

    2015-09-01

    Recent developments in blue emitting laser diodes enable attractive solutions in projection applications using phosphors for efficient light conversion with very high luminance levels. Various commercially available projectors incorporating this technology have entered the market in the past years. While luminous flux levels are still comparable to lamp-based systems, lifetime expectations of classical lamp systems are exceeded by far. OSRAM GmbH has been exploring this technology for several years and has introduced the PHASER® brand name (Phosphor + laser). State-of-the-art is a rotating phosphor wheel excited by blue laser diodes to deliver the necessary primary colors, either sequentially for single-imager projection engines, or simultaneously for 3-panel systems. The PHASER® technology enables flux and luminance scaling, which allows for smaller imagers and therefore cost-efficient projection solutions. The resulting overall efficiency and ANSI lumen specification at the projection screen of these systems is significantly determined by the target color gamut and the light transmission efficiency of the projection system. With increasing power and flux level demand, thermal issues, especially phosphor conversion related, dominate the opto-mechanical system design requirements. These flux levels are a great challenge for all components of an SSL-projection system (SSL:solid-state lighting). OSRAḾs PHASER® light engine platform is constantly expanded towards higher luminous flux levels as well as higher luminance levels for various applications. Recent experiments employ blue laser pump powers of multiple 100 Watts to excite various phosphors resulting in luminous flux levels of more than 40 klm.

  2. Summary of Session Activities: Coordination of Environmental Education Projects

    NASA Technical Reports Server (NTRS)

    Keeler, Michael; Mahootian, Farzad

    1995-01-01

    In this session, we address four fundamental questions related to environmental fields with emphasis on education. These are: What are the goals, objectives, and practical opportunities for coordinating our projects? How can we improve awareness of, interest in, access to, and support the products of our work? How can we build relationships between projects for scientific, educational, technical, and programmatic benefit? How can we evaluate the effectiveness of coordination efforts. In this working session, we produced answers to these questions and proposed a structure for future collaboration.

  3. Effects of heavy metals on the expression of a zinc-inducible metallothionein-III gene and antioxidant enzyme activities in Crassostrea gigas.

    PubMed

    Cong, Ming; Wu, Huifeng; Liu, Xiaoli; Zhao, Jianmin; Wang, Xuan; Lv, Jiasen; Hou, Lin

    2012-10-01

    Sequestration by metallothioneins and antioxidant defense are two kinds of important defense mechanisms employed by mollusks to minimize adverse effects caused by heavy metal contaminants in marine environment. In the present study, a novel metallothionein gene, CgMT-III, was cloned from Crassostrea gigas, consisting of eighteen conserved cysteine residues and encoding a MT III-like protein with two tandem β domains. The expression level of CgMT-III transcript induced by zinc was much higher than that induced by cadmium exposure. It suggested that CgMT-III was perhaps mainly involved in homeostatic control of zinc metabolism, which was distinct from previously identified MTs in C. gigas. Among the tested antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), SOD and GPx showed varying up-regulations in a tissue-specific manner, while CAT activities were inhibited in both gill and hepatopancreas from C. gigas exposed to heavy metals. It can be inferred that CgMT-III was mainly involved in zinc homeostasis, and CgMT-III gene together with CAT enzyme could be potential biomarkers to indicate heavy metal, especially zinc pollution in marine organisms.

  4. Dietary intake, physical activity, body mass index, and childhood asthma in the Third National Health And Nutrition Survey (NHANES III).

    PubMed

    Romieu, Isabelle; Mannino, David M; Redd, Stephen C; McGeehin, Michael A

    2004-07-01

    Childhood asthma may be affected by dietary changes and increased body mass related to a sedentary lifestyle, although the mechanisms are poorly understood. To test this hypothesis, we used data from the National Health and Nutrition Survey (NHANES III) from 1988-1994, including 7,904 children. We analyzed cross-sectional information on body mass index (BMI = weight/height2), physical activity (hr/day viewing television), dietary intake (24-hr recall), and vitamin C intake (60 mg/day). The probability of self-reported asthma or wheezing relating to risk factors was calculated by logistic regression. After controlling for dietary intake, physical activity, and sociodemographic variables, asthma risk was three times higher for children aged 6-16 years in the highest percentiles of BMI (>95th percentile) when compared to children in percentiles 25-49 (OR = 3.44; 95% CI, 1.49-7.96). No increase was observed in children aged 2-5 years. Low vitamin C intake was marginally related to self-reported current wheezing in children aged 6-16 years. Our results show that increased BMI may influence asthma prevalence in children, but further investigation is needed.

  5. Synthesis and in vitro antitumor activity of novel iridium(III) complexes with enantiopure C2-symmetrical vicinal diamine ligands.

    PubMed

    Yang, Qing; Chang, Jun; Song, Jiao; Qian, Meng-Ting; Yu, Jian-Ming; Sun, Xun

    2013-08-15

    Four novel iridium(III) complexes with enantiopure C2-symmetrical vicinal diamine ligands were designed, synthesized, and characterized by FT-IR, NMR, and MS. The cytotoxicities of all of the complexes against the human solid tumor cell lines A2780, A549, KB, and MDA-MB-231 were evaluated. Both R,R-configured complexes (R,R)-5a and (R,R)-5b exhibited more potent or similar activity compared with oxaliplatin, whereas their corresponding (S,S)-isomers (S,S)-5a and (S,S)-5b were found to be mostly inactive. As indicated by the activation of caspase-3, the cleavage of PARP, and the upregulation of p53, the preliminary mechanism studies revealed that the mode of cell death initiated by (R,R)-5a in A2780 cells was predominantly p53-mediated apoptosis. In addition, the structure of (R,R)-5a was unambiguously confirmed through single crystal X-ray structure determination.

  6. Synthesis and Assessment of Antibacterial Activities of Ruthenium(III) Mixed Ligand Complexes Containing 1,10-Phenanthroline and Guanide

    PubMed Central

    Hailemariam, Tizazu

    2016-01-01

    In this work, two complexes of ruthenium(III) ([Ru(phen)2Cl2]Cl·2H2O and [Ru(phen)2(G)Cl]2Cl·H2O) were synthesized from 1,10-phenanthroline alone as well as from both 1,10-phenanthroline and guanide. The synthesis was checked using halide test, conductance measurement, and spectroscopic (ICP-OES, FTIR, and UV/Vis) analysis. Their in vitro antibacterial activities were also investigated on two Gram-positive (Staphylococcus aureus (S. aureus) and methicillin resistant Staphylococcus aureus (MRSA)) and two Gram-negative (Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae)) bacteria. These complexes showed wide-range better activities than the commercially available controls (Chloramphenicol and Ciprofloxacin) against even the most drug resistant K. pneumoniae. [Ru(phen)2(G)Cl]2Cl·H2O inhibited S. aureus, MRSA, E. coli, and K. pneumoniae by 17.5%, 27.4%, 16%, and 52%, respectively, better than Chloramphenicol. It also inhibited these pathogens by 5.9%, 5.1%, 2.3%, and 17.2%, respectively, better than Ciprofloxacin. Similarly, [Ru(Phen)2(Cl)2]Cl·2H2O inhibited these pathogens by 11%, 8.7%, 0.1%, and 31.2%, respectively, better than Chloramphenicol. Therefore, after in vivo cytotoxicity investigations, these compounds can be considered as potential antibiotic drugs. PMID:27833473

  7. The age-mass relation for chromospherically active binaries. III. Lithium depletion in giant components

    NASA Astrophysics Data System (ADS)

    Barrado y Navascues, D.; de Castro, E.; Fernandez-Figueroa, M. J.; Cornide, M.; Garcia Lopez, R. J.

    1998-09-01

    We present a study of the lithium abundances of a sample of evolved components of Chromospherically Active Binary Systems. We show that a significant part of them have lithium excesses, independently of their mass and evolutionary stage. Therefore, it can be concluded that Li abundance does not depend on age for giant components of CABS. These overabundances appear to be closely related to the stellar rotation, and we interpret them as a consequence of the transfer of angular momentum from the orbit to the rotation as the stars evolve in and off the Main Sequence, in a similar way as it happens in the dwarf components of the same systems and in the Tidally Locked Binaries belonging to the Hyades and M67. Based on observations collected with the 2.2\\,m telescope of the German-Spanish Observatorio de Calar Alto (Almeria, Spain), and with the 2.56\\,m Nordic Optical Telescope in the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrof\\'\\i sica de Canarias (La Palma, Spain)

  8. Synthesis, characterization, antioxidative and antitumor activities of solid quercetin rare earth(III) complexes.

    PubMed

    Zhou, J; Wang, L F; Wang, J Y; Tang, N

    2001-01-01

    Eight rare earth metal(II) complexes with quercetin ML3 x 6H2O [L=quercetin (3-OH group deprotonated); M = La, Nd, Eu, Gd, Tb, Dy, Tm and Y] have been synthesized and characterized by elemental analysis, complexometric titration, thermal analysis, conductivity, IR, UV, 1HNMR and fluorescence spectra techniques as well as cyclic voltammetry. The quercetin:metal stoichiometry and the equilibrium stability constant for metal binding to quercetin have been determined. The antioxidative and antitumor activities of quercetin x 2H2O and the complexes were tested by both the MTT and SRB methods. The results show that the suppression ratio of the complexes against the tested tumour cells are superior to quercetin x 2H2O. The property of LaL3 x 6H2O reacting with calf thymus DNA was studied by fluorescence methods. The La-complex binding to DNA has been determined by fluorescence titration in 0.05 M Tris-HCl, 0.5 M NaCl buffer (pH 7.0). The results indicate that the interaction of the complex with DNA is very evident.

  9. A series of binuclear lanthanide(III) complexes: Crystallography, antimicrobial activity and thermochemistry properties studies

    NASA Astrophysics Data System (ADS)

    Zhang, Ying-Ying; Ren, Ning; Xu, Su-Ling; Zhang, Jian-Jun; Zhang, Da-Hai

    2015-02-01

    A series of novel lanthanide complexes with the general formula [Ln(3,4-DClBA)3phen]2 (Ln = Ho(1), Nd(2), Sm(3), Dy(4), Eu(5), Tb(6), Yb(7) and Er(8), 3,4-DClBA = 3,4-dichlorobenzoate, phen = 1,10-phenanthroline) were prepared at room temperature and characterized. The crystal structures of complexes 1-8 have been determined by single crystal X-ray diffraction. These complexes are isomorphous and lanthanide ions are all eight-coordinated to oxygen atoms and nitrogen atoms with distorted square-antiprism geometry. The thermal decomposition mechanism and TG-FTIR spectra of gaseous products of thermal decomposition processes for complexes 1-8 were acquired through TG/DSC-FTIR system. The heat capacities of complexes 1-8 were measured using DSC technology and fitted to a polynomial equation by the least-squares method. Complexes 3-6 display characteristic lanthanide emission bands in the visible region. Meanwhile, these complexes exhibit in good antimicrobial activity against Candida albicans, Escherichia coli, and Staphylococcus aureu.

  10. Simultaneous precipitation of orthophosphate in activated sludge systems with Al(III)

    SciTech Connect

    Gates, D.D.

    1991-01-01

    This dissertation examines the simultaneous precipitation of soluble orthophosphate (SOP) when alum is dosed to an activated sludge aeration basin. The results of batch and continuous flow experimental studies were used to develop a model of this process. This research identified three regions of chemical phosphorus removal, in each of which a different SOP removal mechanism appeared to apply: Region 1 extends to SOP residual concentrations as low as 1.0 mg P/1. In this region the stoichiometric precipitation of Al{sub 0.91}H{sub 2}PO{sub 4} (OH){sub 1.73(s)} is the predominate phosphate removal mechanism. Region 2 includes SOP residual concentrations in the range 0.1-1.0 mg P/1. Phosphate removal in this region is described on the basis of the adsorption of SOP on to aluminum hydroxide solid surfaces. Region 3 includes SOP residual concentrations as low as 0.02 mg P/1. The minimum SOP phosphate concentration that can be reached in this region is controlled by the presence of both aluminum-hydroxyphosphate and aluminum hydroxide solids.

  11. Update on project determining biologically active compounds in milk

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The added health value of raw and pasteurized milk from organic and grass-fed herds is strongly debated because of limited, and often conflicting, scientific data. The Dairy & Functional Foods Research Unit, USDA-ARS-NAA, Wyndmoor, PA has an ongoing project to identify and compare the levels of bio...

  12. Polyguluronate sulfate, polymannuronate sulfate, and their oligosaccharides have antithrombin III- and heparin cofactor II-independent anticoagulant activity

    NASA Astrophysics Data System (ADS)

    Zeng, Xuan; Lan, Ying; Zeng, Pengjiao; Guo, Zhihua; Hao, Cui; Zhang, Lijuan

    2017-04-01

    Cardiovascular disease is the leading causes of death. However, the complications can be treated with heparin and heparinoids, such as heparin pentasaccharide Fondaparinux, dermatan sulfate, and PSS made from alginate extracted from brown seaweeds by chemical sulfation. Alginate is composed of a linear backbone of polymannuronate (PM), polyguluronate (PG), and alternate residues of mannuronic acid and guluronic acid. It is unknown if heparin and sulfated PG (PGS)/PM (PMS) have the same or different anticoagulant molecular targets. In the current study, the anticoagulant activities of PGS, PMS, and their oligosaccharides were directly compared to that of heparin, Fondaparinux, and dermatan sulfate by the activated partial thrombinplastin time (aPTT) assay using normal, antithrombin III (ATIII)-deficient, heparin co-factor II (HCII)-deficient, and ATIII- and HCII-double deficient human plasmas. Our results showed that PGS, PMS, and their oligosaccharides had better anticoagulant activity than that of Fondaparinux in all four human plasmas tested. As expected, heparin was the best anticoagulant in normal plasma. Moreover, PGS, PGS6, PGS12, PGS25, PMS6, PMS12, and PMS25 were better anticoagulants than dermatan sulfate in HCII-deficient plasma. Most strikingly, PGS, PGS12, PGS25, PMS6, PMS12, and PMS25 were better anticoagulants than that of heparin in ATIII- and HCII-double deficient human plasma. The results revealed for the first time that sulfated alginate had ATIII- and HCII-independent anticoagulant activities. Therefore, developing PGS and PMS-based anticoagulants might require to discover their major molecular targets and to develop target-specific anticoagulant assays.

  13. Synthesis, antioxidant activities of the nickel(II), iron(III) and oxovanadium(IV) complexes with N2O2 chelating thiosemicarbazones.

    PubMed

    Bal-Demirci, Tülay; Sahin, Musa; Ozyürek, Mustafa; Kondakçı, Esin; Ulküseven, Bahri

    2014-05-21

    The nickel(II), iron(III) and oxovanadium(IV) complexes of the N2O2 chelating thiosemicarbazones were synthesized using 4-hydroxysalicyladehyde-S-methylthiosemicarbazone and R1-substitute-salicylaldehyde (R1: 4-OH, H) in the presence of Ni(II), Fe(III), VO(IV) ions by the template reaction. The structures of the thiosemicarbazone complexes were characterized by FT-IR, (1)H NMR, elemental, ESI-MS and APCI-MS analysis. The synthesized compounds were screened for their antioxidant capacity by using the cupric reducing antioxidant capacity (CUPRAC) method. Trolox equivalent antioxidant capacity (TEAC) of iron(III) complex, 1c, was measured to be higher than that of the other complexes. Other parameters of antioxidant activity (scavenging effects on •OH, O2(•-) and H2O2) of these compounds were also determined. All the compounds have shown encouraging ROS scavenging activities.

  14. Cr(III), Mn(II), Fe(III), Co(II), Ni(II), Cu(II) and Zn(II) new complexes of 5-aminosalicylic acid: Spectroscopic, thermal characterization and biological activity studies

    NASA Astrophysics Data System (ADS)

    Soliman, Madiha H.; Mohamed, Gehad G.

    2013-04-01

    The complexing behavior of mesalazine (5-aminosalicylic acid; 5-ASA) towards the transition metal ions namely, Cr(III), Mn(II), Fe(III), Co(II), Ni(II), Cu(II) and Zn(II) have been examined by elemental analyses, magnetic measurements, electronic, IR and 1H NMR. Thermal properties and decomposition kinetics of all complexes are investigated. The interpretation, mathematical analyses and evaluation of kinetic parameters of all thermal decomposition stages have been evaluated using Coats-Redfern equation. The free ligand and its metal complexes have been tested in vitro against Aspergillus fumigatus and Candida albicans fungi and Pseudomonas aeruginosa, Escherichia coli, Bacillis subtilies and Staphylococcus aureus bacteria in order to assess their antimicrobial potential. The results indicate that the metal complexes are also found to have more antimicrobial activity than the parent 5-ASA drug.

  15. C-H activation by a mononuclear manganese(III) hydroxide complex: synthesis and characterization of a manganese-lipoxygenase mimic?

    PubMed

    Goldsmith, Christian R; Cole, Adam P; Stack, T Daniel P

    2005-07-13

    Lipoxygenases are mononuclear non-heme metalloenzymes that regio- and stereospecifically convert 1,4-pentadiene subunit-containing fatty acids into alkyl peroxides. The rate-determining step is generally accepted to be hydrogen atom abstraction from the pentadiene subunit of the substrate by an active metal(III)-hydroxide species to give a metal(II)-water species and an organic radical. All known plant and animal lipoxygenases contain iron as the active metal; recently, however, manganese was found to be the active metal in a fungal lipoxygenase. Reported here are the synthesis and characterization of a mononuclear Mn(III) complex, [Mn(III)(PY5)(OH)](CF(3)SO(3))(2) (PY5 = 2,6-bis(bis(2-pyridyl)methoxymethane)pyridine), that reacts with hydrocarbon substrates in a manner most consistent with hydrogen atom abstraction and provides chemical precedence for the proposed reaction mechanism. The neutral penta-pyridyl ligation of PY5 endows a strong Lewis acidic character to the metal center allowing the Mn(III) compound to perform this oxidation chemistry. Thermodynamic analysis of [Mn(III)(PY5)(OH)](2+) and the reduced product, [Mn(II)(PY5)(H(2)O)](2+), estimates the strength of the O-H bond in the metal-bound water in the Mn(II) complex to be 82 (+/-2) kcal mol(-)(1), slightly less than that of the O-H bond in the related reduced iron complex, [Fe(II)(PY5)(MeOH)](2+). [Mn(III)(PY5)(OH)](2+) reacts with hydrocarbon substrates at rates comparable to those of the analogous [Fe(III)(PY5)(OMe)](2+) at 323 K. The crystal structure of [Mn(III)(PY5)(OH)](2+) displays Jahn-Teller distortions that are absent in [Mn(II)(PY5)(H(2)O)](2+), notably a compression along the Mn(III)-OH axis. Consequently, a large internal structural reorganization is anticipated for hydrogen atom transfer, which may be correlated to the lessened dependence of the rate of substrate oxidation on the substrate bond dissociation energy as compared to other metal complexes. The results presented here suggest

  16. Sunlight-driven photo-transformation of bisphenol A by Fe(III) in aqueous solution: Photochemical activity and mechanistic aspects.

    PubMed

    Pan, Meilan; Ding, Jie; Duan, Lin; Gao, Guandao

    2017-01-01

    Iron is one of the most abundant elements in aquatic environments, and plays important roles in the fate and transport of environmental contaminants. Previous studies on the photochemical properties of Fe(III) species have largely focused on complexes formed between Fe(III) and environmental ligands such as natural organic matter (NOM) under UV irradiation, whereas the potentially important roles of hydrolysis species of Fe(III) in Fe(III)-mediated photo-transformation of environmental contaminants under solar light are not fully understood. In this study, the solar light-driven photochemical activities of hydrolysis species of Fe(III) were further explored, using a system containing only 0.5 mM Fe2(SO4)3 and bisphenol A. The important role of colloidal [Fe(OH)3]m, formed from the hydrolysis of Fe(3+), as a core photochemical species of Fe(III) was proposed and verified. Interestingly, O2(-), rather than OH, was identified (via electron spin resonance) as the key active radical responsible for the degradation of bisphenol A. We propose that unlike Fe(OH)(2+), which under UV irradiation can yield OH (Fe(OH)(2+) + hv → Fe(2+) + OH), colloidal [Fe(OH)3]m produces O2(-) even in sunlight ([Fe(OH)3]m + 2O2 + hv → Fe(II) + 2O2(-) + H2O). The fact that Fe(III) can produce strong radicals in sunlight may have important environmental implications.

  17. National BioResource Project-Rat and related activities.

    PubMed

    Serikawa, Tadao; Mashimo, Tomoji; Takizawa, Akiko; Okajima, Ryoko; Maedomari, Naoki; Kumafuji, Kenta; Tagami, Fumi; Neoda, Yuki; Otsuki, Mito; Nakanishi, Satoshi; Yamasaki, Ken-ichi; Voigt, Birger; Kuramoto, Takashi

    2009-07-01

    In order to establish a system to facilitate the systematic collection, preservation, and provision of laboratory rats (Rattus norvegicus) and their derivates, the National BioResource Project-Rat (NBRP-Rat) was launched in July 2002. By the end of 2008, more than 500 rat strains had been collected and preserved as live animals, embryos, or sperm. These rat resources are supplied to biomedical scientists in Japan as well as in other countries. This review article introduces NBRP-Rat and highlights the phenome project, recombinant inbred strains, BAC clone libraries, and the ENU-mutant archive, named the Kyoto University Rat Mutant Archive (KURMA). The future direction of rat resources are also discussed.

  18. Interleukin-8 (IL-8) over-production and autocrine cell activation are key factors in monomethylarsonous acid [MMA(III)]-induced malignant transformation of urothelial cells

    SciTech Connect

    Escudero-Lourdes, C.; Wu, T.; Camarillo, J.M.; Gandolfi, A.J.

    2012-01-01

    The association between chronic human exposure to arsenicals and bladder cancer development is well recognized; however, the underlying molecular mechanisms have not been fully determined. We propose that inflammatory responses can play a pathogenic role in arsenic-related bladder carcinogenesis. In previous studies, it was demonstrated that chronic exposure to 50 nM monomethylarsenous acid [MMA(III)] leads to malignant transformation of an immortalized model of urothelial cells (UROtsa), with only 3 mo of exposure necessary to trigger the transformation-related changes. In the three-month window of exposure, the cells over-expressed pro-inflammatory cytokines (IL-1β, IL-6 and IL-8), consistent with the sustained activation of NFKβ and AP1/c-jun, ERK2, and STAT3. IL-8 was over-expressed within hours after exposure to MMA(III), and sustained over-expression was observed during chronic exposure. In this study, we profiled IL-8 expression in UROtsa cells exposed to 50 nM MMA(III) for 1 to 5 mo. IL-8 expression was increased mainly in cells after 3 mo MMA(III) exposure, and its production was also found increased in tumors derived from these cells after heterotransplantation in SCID mice. UROtsa cells do express both receptors, CXCR1 and CXCR2, suggesting that autocrine cell activation could be important in cell transformation. Supporting this observation and consistent with IL-8 over-expression, CXCR1 internalization was significantly increased after three months of exposure to MMA(III). The expression of MMP-9, cyclin D1, bcl-2, and VGEF was significantly increased in cells exposed to MMA(III) for 3 mo, but these mitogen-activated kinases were significantly decreased after IL-8 gene silencing, together with a decrease in cell proliferation rate and in anchorage-independent colony formation. These results suggest a relevant role of IL-8 in MMA(III)-induced UROtsa cell transformation. -- Highlights: ► IL-8 is over-expressed in human MMA(III)-exposed urothelial

  19. The ERECTA, CLAVATA and class III HD-ZIP Pathways Display Synergistic Interactions in Regulating Floral Meristem Activities

    PubMed Central

    Landau, Udi; Asis, Lior; Eshed Williams, Leor

    2015-01-01

    In angiosperms, the production of flowers marks the beginning of the reproductive phase. At the emergence of flower primordia on the flanks of the inflorescence meristem, the WUSCHEL (WUS) gene, which encodes a homeodomain transcription factor starts to be expressed and establishes de novo stem cell population, founder of the floral meristem (FM). Similarly to the shoot apical meristem a precise spatial and temporal expression pattern of WUS is required and maintained through strict regulation by multiple regulatory inputs to maintain stem cell homeostasis. However, following the formation of a genetically determined fixed number of floral organs, this homeostasis is shifted towards organogenesis and the FM is terminated. In here we performed a genetic study to test how a reduction in ERECTA, CLAVATA and class III HD-ZIP pathways affects floral meristem activity and flower development. We revealed strong synergistic phenotypes of extra flower number, supernumerary whorls, total loss of determinacy and extreme enlargement of the meristem as compared to any double mutant combination indicating that the three pathways, CLV3, ER and HD-ZIPIII distinctively regulate meristem activity and that they act in parallel. Our findings yield several new insights into stem cell-driven development. We demonstrate the crucial requirement for coupling floral meristem termination with carpel formation to ensure successful reproduction in plants. We also show how regulation of meristem size and alternation in spatial structure of the meristem serve as a mechanism to determine flower organogenesis. We propose that the loss of FM determinacy due to the reduction in CLV3, ER and HD-ZIPIII activity is genetically separable from the AGAMOUS core mechanism of meristem termination. PMID:25946150

  20. Heterolytic Activation of C-H Bonds on Cr(III)-O Surface Sites Is a Key Step in Catalytic Polymerization of Ethylene and Dehydrogenation of Propane.

    PubMed

    Conley, Matthew P; Delley, Murielle F; Núñez-Zarur, Francisco; Comas-Vives, Aleix; Copéret, Christophe

    2015-06-01

    We describe the reactivity of well-defined chromium silicates toward ethylene and propane. The initial motivation for this study was to obtain a molecular understanding of the Phillips polymerization catalyst. The Phillips catalyst contains reduced chromium sites on silica and catalyzes the polymerization of ethylene without activators or a preformed Cr-C bond. Cr(II) sites are commonly proposed active sites in this catalyst. We synthesized and characterized well-defined chromium(II) silicates and found that these materials, slightly contaminated with a minor amount of Cr(III) sites, have poor polymerization activity and few active sites. In contrast, chromium(III) silicates have 1 order of magnitude higher activity. The chromium(III) silicates initiate polymerization by the activation of a C-H bond of ethylene. Density functional theory analysis of this process showed that the C-H bond activation step is heterolytic and corresponds to a σ-bond metathesis type process. The same well-defined chromium(III) silicate catalyzes the dehydrogenation of propane at elevated temperatures with activities similar to those of a related industrial chromium-based catalyst. This reaction also involves a key heterolytic C-H bond activation step similar to that described for ethylene but with a significantly higher energy barrier. The higher energy barrier is consistent with the higher pKa of the C-H bond in propane compared to the C-H bond in ethylene. In both cases, the rate-determining step is the heterolytic C-H bond activation.

  1. Contrasting Anticancer Activity of Half-Sandwich Iridium(III) Complexes Bearing Functionally Diverse 2-Phenylpyridine Ligands

    PubMed Central

    2015-01-01

    We report the synthesis, characterization, and antiproliferative activity of 15 iridium(III) half-sandwich complexes of the type [(η5-Cp*)Ir(2-(R′-phenyl)-R-pyridine)Cl] bearing either an electron-donating (−OH, −CH2OH, −CH3) or electron-withdrawing (−F, −CHO, −NO2) group at various positions on the 2-phenylpyridine (2-PhPy) chelating ligand giving rise to six sets of structural isomers. The X-ray crystal structures of [(η5-Cp*)Ir(2-(2′-fluorophenyl)pyridine)Cl] (1) and [(η5-Cp*)Ir(2-(4′-fluorophenyl)pyridine)Cl] (2) exhibit the expected “piano-stool” configuration. DFT calculations showed that substituents caused only localized effects on the electrostatic potential surface of the chelating 2-PhPy ligand of the complexes. Hydrolysis of all complexes is rapid, but readily reversed by addition of NaCl. The complexes show preferential binding to 9-ethylguanine over 9-methyladenine and are active catalysts for the oxidation of NADH to NAD+. Antiproliferative activity experiments in A2780 ovarian, MCF-7 breast, A549 lung, and HCT116 colon cancer cell lines showed IC50 values ranging from 1 to 89 μM, with the most potent complex, [(η5-Cp*)Ir(2-(2′-methylphenyl)pyridine)Cl] (13) (A2780 IC50 = 1.18 μM), being 10× more active than the parent, [(η5-Cp*)Ir(2-phenylpyridine)Cl], and 2× more active than [(η5-CpxPh)Ir(2-phenylpyridine)Cl]. Intriguingly, contrasting biological activities are observed between structural isomers despite exhibiting similar chemical reactivity. For pairs of structural isomers both the nature and position of the functional group can affect the hydrophobicity of the complex. An increase in hydrophobicity resulted in enhanced cellular-iridium accumulation in A2780 ovarian cells, which generally gave rise to an increase in potency. The structural isomers [(η5-Cp*)Ir(2-(4′-fluorophenyl)pyridine)Cl] (2) and [(η5-Cp*)Ir(2-phenyl-5-fluoropyridine)Cl] (4) preferentially localized in the cytosol > membrane and particulate

  2. Toxicity of arsenic (III) and (V) on plant growth, element uptake, and total amylolytic activity of mesquite (Prosopis juliflora x P. velutina).

    PubMed

    Mokgalaka-Matlala, Ntebogeng S; Flores-Tavizón, Edith; Castillo-Michel, Hiram; Peralta-Videa, Jose R; Gardea-Torresdey, Jorge L

    2008-01-01

    The effects of arsenite [As(III)] and arsenate [As(V)] on the growth of roots, stems, and leaves and the uptake of arsenic (As), micro- and macronutrients, and total amylolytic activity were investigated to elucidate the phytotoxicity of As to the mesquite plant (Prosopis juliflora x P. velutina). The plant growth was evaluated by measuring the root and shoot length, and the element uptake was determined using inductively coupled plasma optical emission spectroscopy. The root and leaf elongation decreased significantly with increasing As(III) and As(V) concentrations; whereas, stem elongation remained unchanged. The As uptake increased with increasing As(III) or As(V) concentrations in the medium. Plants treated with 50 mg/L As(III) accumulated up to 920 mg/kg dry weight (d wt) in roots and 522 mg/kg d wt in leaves, while plants exposed to 50 mg/L As(V) accumulated 1980 and 210 mg/kg d wt in roots and leaves, respectively. Increasing the As(V) concentration up to 20 mg/L resulted in a decrease in the total amylolytic activity. On the contrary, total amylolytic activity in As(III)-treated plants increased with increasing As concentration up to 20 mg/L. The macro- and micronutrient concentrations changed in As-treated plants. In shoots, Mo and K were reduced but Ca was increased, while in roots Fe and Ca were increased but K was reduced. These changes reduced the size of the plants, mainly in the As(III)-treated plants; however, there were no visible sign of As toxicity.

  3. Detailed spectroscopic, thermodynamic, and kinetic studies on the protolytic equilibria of Fe(III)cydta and the activation of hydrogen peroxide.

    PubMed

    Brausam, Ariane; Maigut, Joachim; Meier, Roland; Szilágyi, Petra A; Buschmann, Hans-Jürgen; Massa, Werner; Homonnay, Zoltán; van Eldik, Rudi

    2009-08-17

    .1 cm(3) mol(-1). A detailed kinetic study of the effect of the buffer, temperature, and pressure on the reaction of hydrogen peroxide with [Fe(III)(cydta)(H(2)O)](-) was performed using stopped-flow techniques. The reaction was found to consist of two steps and resulted in the formation of a purple Fe(III) side-on-bound peroxo complex [Fe(III)(cydta)(eta(2)-O(2))](3-). The peroxo complex and its degradation products were characterized using Mossbauer spectroscopy. Formation of the purple peroxo complex is only observable above a pH of 9.5. Both reaction steps are affected by specific and general acid catalysis. Two different buffer systems were used to clarify the role of general acid catalysis in these reactions. Mechanistic descriptions and a comparison between the edta and cydta systems are presented. The first reaction step reveals an element of reversibility, which is evident over the whole studied pH range. The positive volume of activation for the forward reaction and the positive entropy of activation for the backward reaction suggest a dissociative interchange mechanism for the reversible end-on binding of hydrogen peroxide to [Fe(III)(cydta)(H(2)O)](-). Deprotonation of the end-on-bound hydroperoxo complex leads to the formation of a seven-coordinate side-on-bound peroxo complex [Fe(III)(cydta)(eta(2)-O(2))](3-), where one carboxylate arm is detached. [Fe(III)(cydta)(eta(2)-O(2))](3-) can be reached by two different pathways, of which one is catalyzed by a base and the other by deprotonated hydrogen peroxide. For both pathways, a small negative volume and entropy of activation was observed, suggesting an associative interchange mechanism for the ring-closure step to the side-on-bound peroxo complex. For the second reaction step, no element of reversibility was found.

  4. Functional Analysis of Plant Defense Suppression and Activation by the Xanthomonas Core Type III Effector XopX.

    PubMed

    Stork, William; Kim, Jung-Gun; Mudgett, Mary Beth

    2015-02-01

    Many phytopathogenic type III secretion effector proteins (T3Es) have been shown to target and suppress plant immune signaling but perturbation of the plant immune system by T3Es can also elicit a plant response. XopX is a "core" Xanthomonas T3E that contributes to growth and symptom development during Xanthomonas euvesicatoria infection of tomato but its functional role is undefined. We tested the effect of XopX on several aspects of plant immune signaling. XopX promoted ethylene production and plant cell death (PCD) during X. euvesicatoria infection of susceptible tomato and in transient expression assays in Nicotiana benthamiana, which is consistent with its requirement for the development of X. euvesicatoria-induced disease symptoms. Additionally, although XopX suppressed flagellin-induced reactive oxygen species, it promoted the accumulation of pattern-triggered immunity (PTI) gene transcripts. Surprisingly, XopX coexpression with other PCD elicitors resulted in delayed PCD, suggesting antagonism between XopX-dependent PCD and other PCD pathways. However, we found no evidence that XopX contributed to the suppression of effector-triggered immunity during X. euvesicatoria-tomato interactions, suggesting that XopX's primary virulence role is to modulate PTI. These results highlight the dual role of a core Xanthomonas T3E in simultaneously suppressing and activating plant defense responses.

  5. Equilibrium unfolding studies of the rat liver methionine adenosyltransferase III, a dimeric enzyme with intersubunit active sites.

    PubMed Central

    Gasset, María; Alfonso, Carlos; Neira, José L; Rivas, Germán; Pajares, María A

    2002-01-01

    The reversible unfolding of rat liver methionine adenosyltransferase dimer by urea under equilibrium conditions has been monitored by fluorescence spectroscopy, CD, size-exclusion chromatography, analytical ultracentrifugation and enzyme activity measurements. The results obtained indicate that unfolding takes place through a three-state mechanism, involving an inactive monomeric intermediate. This intermediate has a 70% native secondary structure, binds less 8-anilinonaphthalene-1-sulphonic acid than the native dimer and has a sedimentation coefficient of 4.24+/-0.15. The variations of free energy in the absence of denaturant [DeltaG(H(2)O)] and its coefficients of urea dependence (m), calculated by the linear extrapolation model, were 36.15+/-2.3 kJ.mol(-1) and 19.87+/-0.71 kJ.mol(-1).M(-1) for the dissociation of the native dimer and 14.77+/-1.63 kJ.mol(-1) and 5.23+/-0.21 kJ.mol(-1).M(-1) for the unfolding of the monomeric intermediate respectively. Thus the global free energy change in the absence of denaturant and the m coefficient were calculated to be 65.69 kJ.mol(-1) and 30.33 kJ.mol(-1).M(-1) respectively. Analysis of the calculated thermodynamical parameters indicate the instability of the dimer in the presence of denaturant, and that the major exposure to the solvent is due to dimer dissociation. Finally, a minimum-folding mechanism for methionine adenosyltransferase III is established. PMID:11772402

  6. Pre-clinical pharmacokinetics and anti-chlamydial activity of salicylidene acylhydrazide inhibitors of bacterial type III secretion.

    PubMed

    Ur-Rehman, Tofeeq; Slepenkin, Anatoly; Chu, Hencelyn; Blomgren, Anders; Dahlgren, Markus K; Zetterström, Caroline E; Peterson, Ellena M; Elofsson, Mikael; Gylfe, Asa

    2012-08-01

    Salicylidene acylhydrazides belong to a class of compounds shown to inhibit bacterial type III secretion (T3S) in pathogenic Gram-negative bacteria. This class of compounds also inhibits growth and replication of Chlamydiae, strict intracellular bacteria that possess a T3S system. In this study a library of 58 salicylidene acylhydrazides was screened to identify inhibitors of Chlamydia growth. Compounds inhibiting growth of both Chlamydia trachomatis and Chlamydophila pneumoniae were tested for cell toxicity and seven compounds were selected for preliminary pharmacokinetic analysis in mice using cassette dosing. Two compounds, ME0177 and ME0192, were further investigated by individual pharmacokinetic analysis. Compound ME0177 had a relatively high peak plasma concentration (C(max)) and area under curve and therefore may be considered for systemic treatment of Chlamydia infections. The other compound, ME0192, had poor pharmacokinetic properties but the highest anti-chlamydial activity in vitro and therefore was tested for topical treatment in a mouse vaginal infection model. ME0192 administered vaginally significantly reduced the infectious burden of C. trachomatis and the number of infected mice.

  7. Synthesis, structure, spectroscopic properties, and antiproliferative activity in vitro of novel osmium(III) complexes with azole heterocycles.

    PubMed

    Stepanenko, Iryna N; Krokhin, Artem A; John, Roland O; Roller, Alexander; Arion, Vladimir B; Jakupec, Michael A; Keppler, Bernhard K

    2008-08-18

    Reactions of (H 2azole) 2[OsCl 6], where Hazole = pyrazole, Hpz, ( 1), indazole, Hind, ( 2), imidazole, Him, ( 3) and benzimidazole, Hbzim, ( 4) with the corresponding azole heterocycle in 1:4 molar ratio in boiling isoamyl alcohol or hexanol-1 afforded novel water-soluble osmium(III) complexes of the type trans-[OsCl 2(Hazole) 4]Cl, where Hazole = Hpz ( 5a), Hind ( 6a), Him ( 7a), and Hbzim ( 9a) in 50-70% ( 5a, 7a, 9a) and 5% ( 6a) yields. The synthesis of 7a was accompanied by a concurrent reaction which led to minor formation (<4%) of cis-[OsCl 2(Him) 4]Cl ( 8). The complexes were characterized by elemental analysis, IR spectroscopy, UV-vis spectroscopy, ESI mass spectrometry, cyclic voltammetry, and X-ray crystallography. 5a, 7a, and 9a were found to possess remarkable antiproliferative activity in vitro against A549 (non-small cell lung carcinoma), CH1 (ovarian carcinoma), and SW480 (colon carcinoma) cells, which was compared with that of related ruthenium compounds trans-[RuCl 2(Hazole) 4]Cl, where Hazole = Hpz (5b), Hind (6b), Him (7b), and Hbzim (9b).

  8. Suppression of the barley uroporphyrinogen III synthase gene by a Ds activation tagging element generates developmental photosensitivity.

    PubMed

    Ayliffe, Michael A; Agostino, Anthony; Clarke, Bryan C; Furbank, Robert; von Caemmerer, Susanne; Pryor, Anthony J

    2009-03-01

    Chlorophyll production involves the synthesis of photoreactive intermediates that, when in excess, are toxic due to the production of reactive oxygen species (ROS). A novel, activation-tagged barley (Hordeum vulgare) mutant is described that results from antisense suppression of a uroporphyrinogen III synthase (Uros) gene, the product of which catalyzes the sixth step in the synthesis of chlorophyll and heme. In homozygous mutant plants, uroporphyrin(ogen) I accumulates by spontaneous cyclization of hydroxyl methylbilane, the substrate of Uros. Accumulation of this tetrapyrrole intermediate results in photosensitive cell death due to the production of ROS. The efficiency of Uros gene suppression is developmentally regulated, being most effective in mature seedling leaves compared with newly emergent leaves. Reduced transcript accumulation of a number of nuclear-encoded photosynthesis genes occurs in the mutant, even under 3% light conditions, consistent with a retrograde plastid-nuclear signaling mechanism arising from Uros gene suppression. A similar set of nuclear genes was repressed in wild-type barley following treatment with a singlet oxygen-generating herbicide, but not by a superoxide generating herbicide, suggesting that the retrograde signaling apparent in the mutant is specific to singlet oxygen.

  9. The type III effector EspF coordinates membrane trafficking by the spatiotemporal activation of two eukaryotic signaling pathways

    PubMed Central

    Alto, Neal M.; Weflen, Andrew W.; Rardin, Matthew J.; Yarar, Defne; Lazar, Cheri S.; Tonikian, Raffi; Koller, Antonius; Taylor, Susan S.; Boone, Charles; Sidhu, Sachdev S.; Schmid, Sandra L.; Hecht, Gail A.; Dixon, Jack E.

    2007-01-01

    Bacterial toxins and effector proteins hijack eukaryotic enzymes that are spatially localized and display rapid signaling kinetics. However, the molecular mechanisms by which virulence factors engage highly dynamic substrates in the host cell environment are poorly understood. Here, we demonstrate that the enteropathogenic Escherichia coli (EPEC) type III effector protein EspF nucleates a multiprotein signaling complex composed of eukaryotic sorting nexin 9 (SNX9) and neuronal Wiskott-Aldrich syndrome protein (N-WASP). We demonstrate that a specific and high affinity association between EspF and SNX9 induces membrane remodeling in host cells. These membrane-remodeling events are directly coupled to N-WASP/Arp2/3–mediated actin nucleation. In addition to providing a biochemical mechanism of EspF function, we find that EspF dynamically localizes to membrane-trafficking organelles in a spatiotemporal pattern that correlates with SNX9 and N-WASP activity in living cells. Thus, our findings suggest that the EspF-dependent assembly of SNX9 and N-WASP represents a novel form of signaling mimicry used to promote EPEC pathogenesis and gastrointestinal disease. PMID:17893247

  10. Recovery Efficiency Test Project: Phase 1, Activity report

    SciTech Connect

    Overbey, W.K. Jr.; Wilkins, D.W.; Keltch, B.; Saradji, B.; Salamy, S.P.

    1988-04-01

    This report is the second volume of the Recovery Efficiency Test Phase I Report of Activities. Volume 1 covered selection, well planning, drilling, coring, logging and completion operations. This volume reports on well testing activities, reclamation activities on the drilling site and access roads, and the results of physical and mechanical properties tests on the oriented core material obtained from a horizontal section of the well. 3 refs., 21 figs., 10 tabs.

  11. Physics and Science Education through Project Activities of University Students and Regional Collaboration

    NASA Astrophysics Data System (ADS)

    Hasegawa, Makoto

    A project team "Rika-Kobo" organized by university students has actively performed various science education activities at primary and secondary schools and other educational facilities as well as in science events in local areas. The activities of this student project team are related to various fields of physics and sciences. In order to provide more attractive activities, the student members prepare original experiment tools and easily-understandable presentation and explanation. Through such activities, the members can have opportunities of obtaining new knowledge and refreshing their already-obtained understandings in related fields of physics and sciences. They can also have chances of improving their skills and abilities such as presentation, problem-finding and solving, which are useful for realizing their career development. The activities of the student project team have been also welcomed by children, parents, teachers and other people in local areas because the activities provide them with opportunities of knowing and learning new knowledge in physics and sciences.

  12. The morphology of flare phenomena, magnetic fields, and electric currents in active regions. III - NOAA active region 6233 (1990 August)

    NASA Technical Reports Server (NTRS)

    De La Beaujardiere, J.-F.; Canfield, Richard C.; Leka, K. D.

    1993-01-01

    We investigate the spatial relationship between vertical electric currents and flare phenomena in NOAA Active Region 6233, which was observed 1990, August 28-31 at Mees Solar Observatory. The two flares studied are the 1N/M1.8 flare on August 28, 22:30 UT and the 1N/M1.6 flare on August 29, 20:35 UT. Using Stokes polarimetry we make magnetograms of the region and compute the vertical current density. Using H-alpha imaging spectroscopy we identify sites of intense nonthermal electron precipitation or of high coronal pressure. The precipitation in these flares is barely strong enough to be detectable. We find that both precipitation and high pressure tend to occur near vertical currents, but that neither phenomenon is cospatial with current maxima. In contrast with the conclusion of other authors, we argue that these observations do not support a current-interruption model for flares, unless the relevant currents are primarily horizontal. The magnetic morphology and temporal evolution of these flares suggest that an erupting filament model may be relevant, but this model does not explicitly predict the relationship between precipitation, high pressure, and vertical currents.

  13. Synthesis, characterization, cytotoxic and antitubercular activities of new gold(I) and gold(III) complexes containing ligands derived from carbohydrates.

    PubMed

    Chaves, Joana Darc Souza; Damasceno, Jaqueline Lopes; Paula, Marcela Cristina Ferreira; de Oliveira, Pollyanna Francielli; Azevedo, Gustavo Chevitarese; Matos, Renato Camargo; Lourenço, Maria Cristina S; Tavares, Denise Crispim; Silva, Heveline; Fontes, Ana Paula Soares; de Almeida, Mauro Vieira

    2015-10-01

    Novel gold(I) and gold(III) complexes containing derivatives of D-galactose, D-ribose and D-glucono-1,5-lactone as ligands were synthesized and characterized by IR, (1)H, and (13)C NMR, high resolution mass spectra and cyclic voltammetry. The compounds were evaluated in vitro for their cytotoxicity against three types of tumor cells: cervical carcinoma (HeLa) breast adenocarcinoma (MCF-7) and glioblastoma (MO59J) and one non-tumor cell line: human lung fibroblasts (GM07492A). Their antitubercular activity was evaluated as well expressed as the minimum inhibitory concentration (MIC90) in μg/mL. In general, the gold(I) complexes were more active than gold(III) complexes, for example, the gold(I) complex (1) was about 8.8 times and 7.6 times more cytotoxic than gold(III) complex (8) in MO59J and MCF-7 cells, respectively. Ribose and alkyl phosphine derivative complexes were more active than galactose and aryl phosphine complexes. The presence of a thiazolidine ring did not improve the cytotoxicity. The study of the cytotoxic activity revealed effective antitumor activities for the gold(I) complexes, being more active than cisplatin in all the tested tumor cell lines. Gold(I) compounds (1), (2), (3), (4) and (6) exhibited relevant antitubercular activity even when compared with first line drugs such as rifampicin.

  14. Development and Evaluation of an Undergraduate Multidisciplinary Project Activity in Engineering and Design

    ERIC Educational Resources Information Center

    Smith, David R.; Cole, Joanne

    2012-01-01

    The School of Engineering and Design Multidisciplinary Project (MDP) at Brunel University is a one week long project based activity involving first year undergraduate students from across the School subject areas of Electronic and Computer Engineering, Mechanical Engineering, Civil Engineering and Design. This paper describes the main aims of the…

  15. Investigation the Scientific Creativity of Gifted Students through Project-Based Activities

    ERIC Educational Resources Information Center

    Karademir, Ersin

    2016-01-01

    In this research, it is aimed to identify the scientific creativity of gifted students through project-based activities. In accordance with this purpose, a study has been carried out with 13 gifted students studying in third and fifth grade. In the study, students have been informed about the project development stages and they have been asked…

  16. 78 FR 52087 - Commercial Filming and Similar Projects and Still Photography Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-22

    ... Service 50 CFR Part 27 RIN 1024-AD30 Commercial Filming and Similar Projects and Still Photography... similar projects and certain still photography activities. DATES: The rule is effective September 23, 2013... of equipment present, and other factors. Authorizes commercial filming and still photography...

  17. Emergency Medical Services for Children: Abstracts of Active Projects FY 1995.

    ERIC Educational Resources Information Center

    National Center for Education in Maternal and Child Health, Arlington, VA.

    This publication provides abstracts of 43 active and 34 completed projects designed to improve pediatric emergency care. The projects were funded by the United States Department of Health and Human Services' Maternal and Child Health Bureau, in collaboration with the United States Department of Transportation's National Highway Traffic Safety…

  18. Emergency Medical Services for Children: Abstracts of Active Projects FY 1994.

    ERIC Educational Resources Information Center

    National Center for Education in Maternal and Child Health, Washington, DC.

    This publication provides abstracts of 32 active and 18 completed projects designed to improve pediatric emergency care. These projects are funded by the U.S. Department of Health and Human Services' Maternal and Child Health Bureau, in collaboration with the U.S. Department of Transportation's National Highway Traffic Safety Administration.…

  19. Emergency Medical Services for Children: Abstracts of Active Projects FY 1996.

    ERIC Educational Resources Information Center

    National Center for Education in Maternal and Child Health, Arlington, VA.

    This publication provides abstracts of 58 active and 42 completed projects designed to improve emergency medical services for children (EMSC). The projects were funded by the United States Department of Health and Human Services' Maternal and Child Health Bureau, in collaboration with the United States Department of Transportation's National…

  20. 40 CFR 47.125 - Eligible and priority projects and activities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 1 2014-07-01 2014-07-01 false Eligible and priority projects and activities. 47.125 Section 47.125 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE NATIONAL ENVIRONMENTAL EDUCATION ACT GRANTS § 47.125 Eligible and priority projects...

  1. 40 CFR 47.125 - Eligible and priority projects and activities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 1 2012-07-01 2012-07-01 false Eligible and priority projects and activities. 47.125 Section 47.125 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE NATIONAL ENVIRONMENTAL EDUCATION ACT GRANTS § 47.125 Eligible and priority projects...

  2. 77 FR 3784 - Recreational Boating Safety Projects, Programs and Activities Funded Under Provisions of the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-25

    ... SECURITY Coast Guard Recreational Boating Safety Projects, Programs and Activities Funded Under Provisions of the Transportation Equity Act for the 21st Century; Accounting of ACTION: Notice. SUMMARY: In 1999... notice to satisfy a requirement of the Act that a detailed accounting of the projects, programs,...

  3. Quality assurance project plan for ground water monitoring activities managed by Westinghouse Hanford Company. Revision 3

    SciTech Connect

    Stauffer, M.

    1995-11-01

    This quality assurance project plan (QAPP) applies specifically to the field activities and laboratory analysis performed for all RCRA groundwater projects conducted by Hanford Technical Services. This QAPP is generic in approach and shall be implemented in conjunction with the specific requirements of individual groundwater monitoring plans.

  4. Positional effects of hydroxy groups on catalytic activity of proton-responsive half-sandwich Cp*Iridium(III) complexes

    SciTech Connect

    Suna, Yuki; Fujita, Etsuko; Ertem, Mehmed Z.; Wang, Wan-Hui; Kambayashi, Hide; Manaka, Yuichi; Muckerman, James T.; Himeda, Yuichiro

    2014-11-12

    Proton-responsive half-sandwich Cp*Ir(III) complexes possessing a bipyridine ligand with two hydroxy groups at the 3,3'-, 4,4'-, 5,5'- or 6,6'-positions (3DHBP, 4DHBP, 5DHBP, or 6DHBP) were systematically investigated. UV-vis titration data provided average pK a values of the hydroxy groups on the ligands. Both hydroxy groups were found to deprotonate in the pH 4.6–5.6 range for the 4–6DHBP complexes. One of the hydroxy groups of the 3DHBP complex exhibited the low pKa value of < 0.4 because the deprotonation is facilitated by the strong intramolecular hydrogen bond formed between the generated oxyanion and the remaining hydroxy group, which in turn leads to an elevated pKa value of ~13.6 for the second deprotonation step. The crystal structures of the 4– and 6DHBP complexes obtained from basic aqueous solutions revealed their deprotonated forms. The intramolecular hydrogen bond in the 3DHBP complex was also observed in the crystal structures. The catalytic activities of these complexes in aqueous phase reactions, at appropriate pH, for hydrogenation of carbon dioxide (pH 8.5), dehydrogenation of formic acid (pH 1.8), transfer hydrogenation reactions using formic acid/formate as a hydrogen source (pH 7.2 and 2.6) were investigated to compare the positional effects of the hydroxy groups. The 4– and 6DHBP complexes exhibited remarkably enhanced catalytic activities under basic conditions because of the resonance effect of the strong electrondonating oxyanions, whereas the 5DHBP complex exhibited negligible activity despite the presence of electron-donating groups. The 3DHBP complex exhibited relatively high catalytic activity at low pH owing to the one strong electron-donating oxyanion group stabilized by the intramolecular hydrogen bond. DFT calculations were employed to study the mechanism of CO₂ hydrogenation by the 4DHBP and 6DHBP complexes, and comparison of the activation free energies of the H₂ heterolysis and CO

  5. Positional effects of hydroxy groups on catalytic activity of proton-responsive half-sandwich Cp*Iridium(III) complexes

    DOE PAGES

    Suna, Yuki; Fujita, Etsuko; Ertem, Mehmed Z.; ...

    2014-11-12

    Proton-responsive half-sandwich Cp*Ir(III) complexes possessing a bipyridine ligand with two hydroxy groups at the 3,3'-, 4,4'-, 5,5'- or 6,6'-positions (3DHBP, 4DHBP, 5DHBP, or 6DHBP) were systematically investigated. UV-vis titration data provided average pK a values of the hydroxy groups on the ligands. Both hydroxy groups were found to deprotonate in the pH 4.6–5.6 range for the 4–6DHBP complexes. One of the hydroxy groups of the 3DHBP complex exhibited the low pKa value of < 0.4 because the deprotonation is facilitated by the strong intramolecular hydrogen bond formed between the generated oxyanion and the remaining hydroxy group, which in turn leadsmore » to an elevated pKa value of ~13.6 for the second deprotonation step. The crystal structures of the 4– and 6DHBP complexes obtained from basic aqueous solutions revealed their deprotonated forms. The intramolecular hydrogen bond in the 3DHBP complex was also observed in the crystal structures. The catalytic activities of these complexes in aqueous phase reactions, at appropriate pH, for hydrogenation of carbon dioxide (pH 8.5), dehydrogenation of formic acid (pH 1.8), transfer hydrogenation reactions using formic acid/formate as a hydrogen source (pH 7.2 and 2.6) were investigated to compare the positional effects of the hydroxy groups. The 4– and 6DHBP complexes exhibited remarkably enhanced catalytic activities under basic conditions because of the resonance effect of the strong electrondonating oxyanions, whereas the 5DHBP complex exhibited negligible activity despite the presence of electron-donating groups. The 3DHBP complex exhibited relatively high catalytic activity at low pH owing to the one strong electron-donating oxyanion group stabilized by the intramolecular hydrogen bond. DFT calculations were employed to study the mechanism of CO₂ hydrogenation by the 4DHBP and 6DHBP complexes, and comparison of the activation free energies of the H₂ heterolysis and CO₂ insertion steps

  6. Project ACE Activity Sets. Book II: Grades 6 and 7.

    ERIC Educational Resources Information Center

    Eden City Schools, NC.

    The document contains eight activity sets suitable for grades 6 and 7. Topics focus on governmental, social, and educational systems in foreign countries. Each activity set contains background reading materials, resources, concepts, general objectives, and instructional objectives. Grade 6 sets are "Soviet Youth Organizations,""How…

  7. Albeni Falls Wildlife Mitigation Project : Annual Report of Mitigation Activities.

    SciTech Connect

    Entz, Ray D.

    2001-04-01

    The Albeni Falls Interagency Work Group was actively involved in implementing wildlife mitigation activities in 2000. The Work Group met each quarter to discuss management and budget issues affecting Albeni Falls wildlife mitigation. Members of the Work Group protected a total of 1,242 acres of wetland habitat in 2000. The total amount of wildlife habitat protected for Albeni Falls mitigation is approximately 4,190 acres (4,630 Habitat Units). Approximately 16% of the total wildlife habitat lost has been mitigated. Land management activities were limited in 2000 as protection opportunities took up most staff time. Administrative activities increased in 2000 as funding was more evenly distributed among Work Group members. As a result, implementation is expected to continue to increase in the coming year. Land management and monitoring and evaluation activities will increase in 2001 as site-specific management plans are completed and implemented.

  8. Rh(III)-Catalyzed Cyclopropanation Initiated by C–H Activation: Ligand Development Enables a Diastereoselective [2 + 1] Annulation of N-Enoxyphthalimides and Alkenes

    PubMed Central

    2015-01-01

    N-Enoxyphthalimides undergo a Rh(III)-catalyzed C–H activation initiated cyclopropanation of electron deficient alkenes. The reaction is proposed to proceed via a directed activation of the olefinic C–H bond followed by two migratory insertions, first across the electron-deficient alkene and then by cyclization back onto the enol moiety. A newly designed isopropylcyclopentadienyl ligand drastically improves yield and diastereoselectivity. PMID:25093811

  9. Activity-Dependent Callosal Axon Projections in Neonatal Mouse Cerebral Cortex

    PubMed Central

    Tagawa, Yoshiaki; Hirano, Tomoo

    2012-01-01

    Callosal axon projections are among the major long-range axonal projections in the mammalian brain. They are formed during the prenatal and early postnatal periods in the mouse, and their development relies on both activity-independent and -dependent mechanisms. In this paper, we review recent findings about the roles of neuronal activity in callosal axon projections. In addition to the well-documented role of sensory-driven neuronal activity, recent studies using in utero electroporation demonstrated an essential role of spontaneous neuronal activity generated in neonatal cortical circuits. Both presynaptic and postsynaptic neuronal activities are critically involved in the axon development. Studies have begun to reveal intracellular signaling pathway which works downstream of neuronal activity. We also review several distinct patterns of neuronal activity observed in the developing cerebral cortex, which might play roles in activity-dependent circuit construction. Such neuronal activity during the neonatal period can be disrupted by genetic factors, such as mutations in ion channels. It has been speculated that abnormal activity caused by such factors may affect activity-dependent circuit construction, leading to some developmental disorders. We discuss a possibility that genetic mutation in ion channels may impair callosal axon projections through an activity-dependent mechanism. PMID:23213574

  10. Front Range Infrastructure Resources Project: water-resources activities

    USGS Publications Warehouse

    Robson, Stanley G.; Heiny, Janet S.

    1998-01-01

    Infrastructure, such as roads, buildings, airports, and dams, is built and maintained by use of large quantities of natural resources such as aggregate (sand and gravel), energy, and water. As urban area expand, local sources of these resource are becoming inaccessible (gravel cannot be mined from under a subdivision, for example), or the cost of recovery of the resource becomes prohibitive (oil and gas drilling in urban areas is costly), or the resources may become unfit for some use (pollution of ground water may preclude its use as a water supply). Governmental land-use decision and environmental mandates can further preclude development of natural resources. If infrastructure resources are to remain economically available. current resource information must be available for use in well-reasoned decisions bout future land use. Ground water is an infrastructure resource that is present in shallow aquifers and deeper bedrock aquifers that underlie much of the 2,450-square-mile demonstration area of the Colorado Front Range Infrastructure Resources Project. In 1996, mapping of the area's ground-water resources was undertaken as a U.S. Geological Survey project in cooperation with the Colorado Department of Natural Resources, Division of Water Resources, and the Colorado Water Conservation Board.

  11. 78 FR 8192 - Agency Information Collection Activities: Comment Request; Education and Human Resources Project...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-05

    ... From the Federal Register Online via the Government Publishing Office NATIONAL SCIENCE FOUNDATION Agency Information Collection Activities: Comment Request; Education and Human Resources Project... study will assess the implementation of resources, models, and technologies to determine how and...

  12. The VPS-20 subunit of the endosomal sorting complex ESCRT-III exhibits an open conformation in the absence of upstream activation.

    PubMed

    Schuh, Amber L; Hanna, Michael; Quinney, Kyle; Wang, Lei; Sarkeshik, Ali; Yates, John R; Audhya, Anjon

    2015-03-15

    Members of the endosomal sorting complex required for transport (ESCRT) machinery function in membrane remodelling processes during multivesicular endosome (MVE) biogenesis, cytokinesis, retroviral budding and plasma membrane repair. During luminal vesicle formation at endosomes, the ESCRT-II complex and the ESCRT-III subunit vacuolar protein sorting (VPS)-20 play a specific role in regulating assembly of ESCRT-III filaments, which promote vesicle scission. Previous work suggests that Vps20 isoforms, like other ESCRT-III subunits, exhibits an auto-inhibited closed conformation in solution and its activation depends on an association with ESCRT-II specifically at membranes [1]. However, we show in the present study that Caenorhabditis elegans ESCRT-II and VPS-20 interact directly in solution, both in cytosolic cell extracts and in using recombinant proteins in vitro. Moreover, we demonstrate that purified VPS-20 exhibits an open extended conformation, irrespective of ESCRT-II binding, in contrast with the closed auto-inhibited architecture of another ESCRT-III subunit, VPS-24. Our data argue that individual ESCRT-III subunits adopt distinct conformations, which are tailored for their specific functions during ESCRT-mediated membrane reorganization events.

  13. UV and visible activation of Cr(III)-doped TiO2 catalyst prepared by a microwave-assisted sol-gel method during MCPA degradation.

    PubMed

    Mendiola-Alvarez, S Y; Guzmán-Mar, J L; Turnes-Palomino, G; Maya-Alejandro, F; Hernández-Ramírez, A; Hinojosa-Reyes, L

    2016-11-10

    Photocatalytic degradation of 4-chloro-2-methylphenoxyacetic acid (MCPA) in aqueous solution using Cr(III)-doped TiO2 under UV and visible light was investigated. The semiconductor material was synthesized by a microwave-assisted sol-gel method with Cr(III) doping contents of 0.02, 0.04, and 0.06 wt%. The catalyst was characterized using X-ray powder diffraction (XRD), scanning electron microscopy (SEM), nitrogen physisorption, UV-Vis diffuse reflectance spectroscopy (DRS), and atomic absorption spectroscopy (AAS). The photocatalytic activity for the photodegradation of MCPA was followed by reversed-phase high-performance liquid chromatography (HPLC) and total organic carbon (TOC) analysis. The intermediates formed during degradation were identified using gas chromatography-mass spectrometry (GC-MS). Chloride ion evolution was measured by ion chromatography. Characterization results showed that Cr(III)-doped TiO2 materials possessed a small crystalline size, high surface area, and mesoporous structure. UV-Vis DRS showed enhanced absorption in the visible region as a function of the Cr(III) concentration. The Cr(III)-doped TiO2 catalyst with 0.04 wt% of Cr(III) was more active than bare TiO2 for the degradation of MCPA under both UV and visible light. The intermediates identified during MCPA degradation were 4-chloro-2-methylphenol (CMP), 2-(4-hydroxy-2-methylphenoxy) acetic acid (HMPA), and 2-hydroxybuta-1,3-diene-1,4-diyl-bis (oxy)dimethanol (HBDM); the formation of these intermediates depended on the radiation source.

  14. EMSP project summary (Project ID: 60077): Development of nuclear analysis capabilities for DOE waste management activities

    SciTech Connect

    Parks, C.V.; Rearden, B.T.; DeHart, M.D.; Broadhead, B.L.; Hopper, C.M.; Petrie, L.M.

    2000-02-01

    The objective of this project is to develop and demonstrate prototypical analysis capabilities that can be used by nuclear safety analysis practitioners to: (1) demonstrate a more thorough understanding of the underlying physics phenomena that can lead to improved reliability and defensibility of safety evaluations; and (2) optimize operations related to the handling, storage, transportation, and disposal of fissile material and DOE spent fuel. To address these problems, this project has been investigating the implementation of sensitivity and uncertainty methods within existing Monte Carlo codes used for criticality safety analyses. It is also investigating the use of a new deterministic code that allows for specification of arbitrary grids to accurately model geometric details required in a criticality safety analysis. This capability can facilitate improved estimations of the required subcritical margin and potentially enable the use of a broader range of experiments in the validation process. The new arbitrary-grid radiation transport code will also enable detailed geometric modeling valuable for improved accuracy in application to a myriad of other problems related to waste characterization. Application to these problems will also be explored.

  15. Effects of mutations in the Exo III motif of the herpes simplex virus DNA polymerase gene on enzyme activities, viral replication, and replication fidelity.

    PubMed Central

    Hwang, Y T; Liu, B Y; Coen, D M; Hwang, C B

    1997-01-01

    The herpes simplex virus DNA polymerase catalytic subunit, which has intrinsic polymerase and 3'-5' exonuclease activities, contains sequence motifs that are homologous to those important for 3'-5' exonuclease activity in other polymerases. The role of one such motif, Exo III, was examined in this study. Mutated polymerases containing either a single tyrosine-to-histidine change at residue 577 or this change plus an aspartic acid-to-alanine at residue 581 in the Exo III motif exhibited defective or undetectable exonuclease activity, respectively, yet retained substantial polymerase activity. Despite the defects in exonuclease activity, the mutant polymerases were able to support viral replication in transient complementation assays, albeit inefficiently. Viruses replicated via the action of these mutant polymerases exhibited substantially increased frequencies of mutants resistant to ganciclovir. Furthermore, when the Exo III mutations were incorporated into the viral genome, the resulting mutant viruses displayed only modestly defect in replication in Vero cells and exhibited substantially increased mutation frequencies. The results suggest that herpes simplex virus can replicate despite severely impaired exonuclease activity and that the 3'-5' exonuclease contributes substantially to the fidelity of viral DNA replication. PMID:9311864

  16. Mono- and dinuclear manganese(III) complexes showing efficient catechol oxidase activity: syntheses, characterization and spectroscopic studies.

    PubMed

    Banu, Kazi Sabnam; Chattopadhyay, Tanmay; Banerjee, Arpita; Mukherjee, Madhuparna; Bhattacharya, Santanu; Patra, Goutam Kumar; Zangrando, Ennio; Das, Debasis

    2009-10-28

    Four side-off compartmental ligands L1-L4 [L1 = N,N'-ethylenebis(3-formyl-5-methyl-salicylaldimine), L2 = N,N'-1-methylethylenebis(3-formyl-5-methylsalicylaldimine), L3 = N,N'-1,1-dimethylethylenebis(3-formyl-5-methylsalicylaldimine) and L4= N,N'-cyclohexenebis(3-formyl-5-methylsalicylaldimine)] having two binding sites, N2O2 and O4, have been chosen to synthesize mononuclear and dinuclear manganese(III) complexes with the aim to study their catecholase activity using 3,5-di-tert-butylcatechol (3,5-DTBC) as substrate in the presence of molecular oxygen. In all cases only mononuclear manganese complexes (1-4) were obtained, with manganese coordination taking place at the N2O2 binding site only, irrespective of the amount of manganese salt used. All these complexes have been characterized by routine physico-chemical techniques. Complex MnL2Cl.4H2O (2) has further been structurally characterized by X-ray single crystal structure analysis. Four dinuclear manganese complexes, 5-8, were obtained after condensing the two pending formyl groups on each ligand (L1-L4) with aniline followed by reaction with MnCl2 to put the second Mn atom onto another N2O2 site. The catalytic activity of all complexes 1-8 has been investigated following the oxidation of 3,5-di-tert-butylcatechol (3,5-DTBC) to 3,5-di-tert-butylbenzoquinone (3,5-DTBQ) with molecular oxygen in two different solvents, methanol and acetonitrile. The study reveals that the catalytic activity is influenced by the solvent and to a significant extent by the backbone of the diamine and the behavior seems to be related mainly to steric rather than electronic factors. Experimental data suggest that a correlation, the lower the E(1/2) value the higher the catalytic activity, can be drawn between E(1/2) and Vmax of the complexes in a particular solvent. The EPR measurements suggest that the catalytic property of the complexes is related to the metal center(s) participation rather than to a radical mechanism.

  17. Kynurenine Aminotransferase III and Glutamine Transaminase L Are Identical Enzymes that have Cysteine S-Conjugate β-Lyase Activity and Can Transaminate l-Selenomethionine*

    PubMed Central

    Pinto, John T.; Krasnikov, Boris F.; Alcutt, Steven; Jones, Melanie E.; Dorai, Thambi; Villar, Maria T.; Artigues, Antonio; Li, Jianyong; Cooper, Arthur J. L.

    2014-01-01

    Three of the four kynurenine aminotransferases (KAT I, II, and IV) that synthesize kynurenic acid, a neuromodulator, are identical to glutamine transaminase K (GTK), α-aminoadipate aminotransferase, and mitochondrial aspartate aminotransferase, respectively. GTK/KAT I and aspartate aminotransferase/KAT IV possess cysteine S-conjugate β-lyase activity. The gene for the former enzyme, GTK/KAT I, is listed in mammalian genome data banks as CCBL1 (cysteine conjugate beta-lyase 1). Also listed, despite the fact that no β-lyase activity has been assigned to the encoded protein in the genome data bank, is a CCBL2 (synonym KAT III). We show that human KAT III/CCBL2 possesses cysteine S-conjugate β-lyase activity, as does mouse KAT II. Thus, depending on the nature of the substrate, all four KATs possess cysteine S-conjugate β-lyase activity. These present studies show that KAT III and glutamine transaminase L are identical enzymes. This report also shows that KAT I, II, and III differ in their ability to transaminate methyl-l-selenocysteine (MSC) and l-selenomethionine (SM) to β-methylselenopyruvate (MSP) and α-ketomethylselenobutyrate, respectively. Previous studies have identified these seleno-α-keto acids as potent histone deacetylase inhibitors. Methylselenol (CH3SeH), also purported to have chemopreventive properties, is the γ-elimination product of SM and the β-elimination product of MSC catalyzed by cystathionine γ-lyase (γ-cystathionase). KAT I, II, and III, in part, can catalyze β-elimination reactions with MSC generating CH3SeH. Thus, the anticancer efficacy of MSC and SM will depend, in part, on the endogenous expression of various KAT enzymes and cystathionine γ-lyase present in target tissue coupled with the ability of cells to synthesize in situ either CH3SeH and/or seleno-keto acid metabolites. PMID:25231977

  18. Behavior‐dependent activity patterns of GABAergic long‐range projecting neurons in the rat hippocampus

    PubMed Central

    Micklem, Ben; Borhegyi, Zsolt; Swiejkowski, Daniel A.; Valenti, Ornella; Viney, Tim J.; Kotzadimitriou, Dimitrios; Klausberger, Thomas

    2017-01-01

    ABSTRACT Long‐range glutamatergic and GABAergic projections participate in temporal coordination of neuronal activity in distributed cortical areas. In the hippocampus, GABAergic neurons project to the medial septum and retrohippocampal areas. Many GABAergic projection cells express somatostatin (SOM+) and, together with locally terminating SOM+ bistratified and O‐LM cells, contribute to dendritic inhibition of pyramidal cells. We tested the hypothesis that diversity in SOM+ cells reflects temporal specialization during behavior using extracellular single cell recording and juxtacellular neurobiotin‐labeling in freely moving rats. We have demonstrated that rare GABAergic projection neurons discharge rhythmically and are remarkably diverse. During sharp wave‐ripples, most projection cells, including a novel SOM+ GABAergic back‐projecting cell, increased their activity similar to bistratified cells, but unlike O‐LM cells. During movement, most projection cells discharged along the descending slope of theta cycles, but some fired at the trough jointly with bistratified and O‐LM cells. The specialization of hippocampal SOM+ projection neurons complements the action of local interneurons in differentially phasing inputs from the CA3 area to CA1 pyramidal cell dendrites during sleep and wakefulness. Our observations suggest that GABAergic projection cells mediate the behavior‐ and network state‐dependent binding of neuronal assemblies amongst functionally‐related brain regions by transmitting local rhythmic entrainment of neurons in CA1 to neuronal populations in other areas. © 2016 The Authors Hippocampus Published by Wiley Periodicals, Inc. PMID:27997999

  19. Wisconsin Technical College System Equity Staff Development Workshops and Services--Phase III. Final Report.

    ERIC Educational Resources Information Center

    Baldus, Lorayne; Nelson, Orville

    The Wisconsin Technical College System (WTCS) Phase III Equity Staff Development project was conducted to determine strategies to eliminate sex bias and sex role stereotyping throughout the WTCS. The following project activities were conducted: (1) the WTCS sex equity advisory committee was formed to provide continuity and direction for state…

  20. RNA Polymerase III transcription in higher plants: Annual performance report, December 20, 1986 through December 15, 1987

    SciTech Connect

    Hall, B.D.

    1987-01-01

    tDNA-dependent Pol III transcription is not observed directly in extracts of wheat embryo cells or nuclei. This project seeks to purify wheat germ RNA Polymerase III, purify wheat factor TFIIIC based upon tDNA binding activity, and by using the two above components plus a specific cloned tDNA template, to screen extracts for the one missing component.

  1. Self-face recognition shares brain regions active during proprioceptive illusion in the right inferior fronto-parietal superior longitudinal fasciculus III network.

    PubMed

    Morita, Tomoyo; Saito, Daisuke N; Ban, Midori; Shimada, Koji; Okamoto, Yuko; Kosaka, Hirotaka; Okazawa, Hidehiko; Asada, Minoru; Naito, Eiichi

    2017-04-21

    Proprioception is somatic sensation that allows us to sense and recognize position, posture, and their changes in our body parts. It pertains directly to oneself and may contribute to bodily awareness. Likewise, one's face is a symbol of oneself, so that visual self-face recognition directly contributes to the awareness of self as distinct from others. Recently, we showed that right-hemispheric dominant activity in the inferior fronto-parietal cortices, which are connected by the inferior branch of the superior longitudinal fasciculus (SLF III), is associated with proprioceptive illusion (awareness), in concert with sensorimotor activity. Herein, we tested the hypothesis that visual self-face recognition shares brain regions active during proprioceptive illusion in the right inferior fronto-parietal SLF III network. We scanned brain activity using functional magnetic resonance imaging while twenty-two right-handed healthy adults performed two tasks. One was a proprioceptive illusion task, where blindfolded participants experienced a proprioceptive illusion of right hand movement. The other was a visual self-face recognition task, where the participants judged whether an observed face was their own. We examined whether the self-face recognition and the proprioceptive illusion commonly activated the inferior fronto-parietal cortices connected by the SLF III in a right-hemispheric dominant manner. Despite the difference in sensory modality and in the body parts involved in the two tasks, both tasks activated the right inferior fronto-parietal cortices, which are likely connected by the SLF III, in a right-side dominant manner. Here we discuss possible roles for right inferior fronto-parietal activity in bodily awareness and self-awareness.

  2. Support of an Active Science Project by a Large Information System: Lessons for the EOS Era

    NASA Technical Reports Server (NTRS)

    Angelici, Gary L.; Skiles, J. W.; Popovici, Lidia Z.

    1993-01-01

    The ability of large information systems to support the changing data requirements of active science projects is being tested in a NASA collaborative study. This paper briefly profiles both the active science project and the large information system involved in this effort and offers some observations about the effectiveness of the project support. This is followed by lessons that are important for those participating in large information systems that need to support active science projects or that make available the valuable data produced by these projects. We learned in this work that it is difficult for a large information system focused on long term data management to satisfy the requirements of an on-going science project. For example, in order to provide the best service, it is important for all information system staff to keep focused on the needs and constraints of the scientists in the development of appropriate services. If the lessons learned in this and other science support experiences are not applied by those involved with large information systems of the EOS (Earth Observing System) era, then the final data products produced by future science projects may not be robust or of high quality, thereby making the conduct of the project science less efficacious and reducing the value of these unique suites of data for future research.

  3. Front Range Infrastructure Resources Project; energy resources activities

    USGS Publications Warehouse

    ,

    1998-01-01

    Oil, natural gas, and coal (energy resources) have been produced from rocks in the Front Range of Colorado and Wyoming for more than a century, and significant quantities of oil and gas continue to be developed in the study area of the U.S. Geological Survey (USGS) Front Range Infrastructure Resources Project (fig. 1). As an infrastructure resource, energy resources helped to fuel past development of (1) urban areas in the Front Range as well as (2) some of the manufacturing and mining upon which the urban centers were built and thrived. At present, much of the oil and gas extracted from rocks beneath the Front Range urban corridor is used locally; the people living in the urban area provide a need and viable marketplace for these commodities.

  4. 25 CFR 170.623 - How are IRR Program projects and activities included in a self-governance agreement?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 1 2011-04-01 2011-04-01 false How are IRR Program projects and activities included in a... Contracts and Agreements Under Isdeaa § 170.623 How are IRR Program projects and activities included in a self-governance agreement? To include an IRR Program project or activity in a self-governance...

  5. Subsurface mass migration at active volcanoes: what we learnt from the VOLUME project

    NASA Astrophysics Data System (ADS)

    Saccorotti, G.; Volume Team

    2009-04-01

    Movements of multiphase fluids beneath active volcanoes are generally detected at the surface in terms of changes in geophysical and geochemical observables. The prompt detection and interpretation of such signals thus represent a crucial step toward the short-term evaluation of volcanic hazard. Funded through the European 6th framework program, the VOLUME project joined 19 institutions from 6 EU and 5 extra-european countries under the common goal of improving our understanding of how subsurface mass movement manifests itself at the surface, in turn revealing the significance of such movements as precursors to impending eruptions. We integrated high-end experimental procedures with a robust modeling framework to address some of the most relevant issues of modern, quantitative volcanology. In particular, our studies focused on: (i) Unrevealing the complex interplay between hydrothermal and magmatic fluids in generating the observed geophysical / geochemical signals, (ii) Detailing the location, geometry and dynamics of magma pathways and storage zones (iii) Probing variations of the elastic parameters of volcanic media in response to stress changes induced by mass migration, and (iv) Developing a robust computational framework for forward-modelling the geophysical observables resulting from the dynamics of multiphase magmatic systems. VOLUME activities developed at both european and extra-european volcanoes. We present here the most striking results obtained at two italian test-sites, namely Etna and Campi Flegrei, for which we had available data sets of unprecedented sensitivity and temporal resolution. Results from Etna include a) mapping of the shallow plumbing system from Moment-Tensor inversion of broadband seismic signal, b) the detection of deep magma intrusion from inversion of joint gravity-tremor anomalies; c) the measurement of changes in both elastic anisotropy and seismic velocity concomitant to the waning stage of the 2002 NE flank lava effusion; and

  6. An Overview of Follow-On Testing Activities of the A-3 Subscale Diffuser Test Project

    NASA Technical Reports Server (NTRS)

    Ryan, James E.

    2009-01-01

    An overview of NASA Stennis Space Center's (SSC) A-3 Subscale Diffuser Test (SDT) Project is presented. The original scope of the SDT Project, conducted from April 2007 to January 2008, collected data to support mitigation of risk associated with design and procurement activities of the A-3 Test Stand Project, an effort to construct a simulated altitude test facility at SSC in support of NASA's Constellation Program. Follow-on tests were conducted from May 2008 through August 2009, utilizing the SDT test setup as a testbed for additional risk mitigation activities. Included are descriptions of the Subscale Diffuser (SD) test article, the test facility configuration, and test approaches.

  7. Design Activity in the Software Cost Reduction Project.

    DTIC Science & Technology

    1986-08-18

    PM Physical Model S G System Generation SS Shared Services SU System Utilities . NOV M N 1600SEP A 0 JUL TOTAL 14000 MAAR cc 100 FEB :IESGN 0o 10000...iy---- .... ;’ TESTING Jan 78 Jan 79 Jan 80 Jan 81 Jan 82 Jan 83 Jan 84 Jan 85 M3ITH Fig. 7 - Shared services activities A F 0 U E C 1600 G B T...DISCUSSING 200M Jan 78 Jan 79 Jan 80 Jan 81 Jan 82 Jan 83 Jan 84 Jan 85 Fig 13 - Shared services design activities 5.~ S% 12 ......,ooU7 . . NRL REPORT 8974 A

  8. Mixed ligand complexes of cobalt(III) and iron(III) containing N2O2-chelating Schiff base: Synthesis, characterisation, antimicrobial activity, antioxidant and DFT study

    NASA Astrophysics Data System (ADS)

    Pramanik, Harun A. R.; Paul, Pradip C.; Mondal, Paritosh; Bhattacharjee, Chira R.

    2015-11-01

    Six mixed ligand complexes, namely, [Co(acac)L1] (1), [Fe(acac)L1] (2), [Co(acac)L2] (3), [Fe(acac)L2] (4), [Co(acac)L3] (5), and [Fe(acac)L3] (6) (H2L1 = NN/-bis(salicylidene)-trans 1,2 diaminocyclohexane, H2L2 = NN/-bis(salicylidene)-1,2 phenylenediamine, H2L3 = NN/-bis(salicylidene)-4-methyl-1,2-phenylenediamine) were synthesised and characterized using elemental analysis, IR spectra, UV-Vis spectra, mass spectra, magnetic susceptibility measurements, 1H and 13C NMR spectroscopy, thermogravimetric analysis. The molar conductance measurement confirmed the non-electrolytic nature of the complexes in DMF solution. Antioxidant activity of the complexes was studied using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging method. Biological studies of the complexes have been carried out in vitro for antimicrobial activity against some selected gram-positive and gram-negative bacteria. DFT calculations were performed using GAUSSIAN 09 program to ascertain the stable electronic structure, HOMO-LUMO energy gap, chemical hardness and dipole moment of the complexes.

  9. The β2 clamp in the Mycobacterium tuberculosis DNA polymerase III αβ2ε replicase promotes polymerization and reduces exonuclease activity

    PubMed Central

    Gu, Shoujin; Li, Wenjuan; Zhang, Hongtai; Fleming, Joy; Yang, Weiqiang; Wang, Shihua; Wei, Wenjing; Zhou, Jie; Zhu, Guofeng; Deng, Jiaoyu; Hou, Jian; Zhou, Ying; Lin, Shiqiang; Zhang, Xian-En; Bi, Lijun

    2016-01-01

    DNA polymerase III (DNA pol III) is a multi-subunit replication machine responsible for the accurate and rapid replication of bacterial genomes, however, how it functions in Mycobacterium tuberculosis (Mtb) requires further investigation. We have reconstituted the leading-strand replication process of the Mtb DNA pol III holoenzyme in vitro, and investigated the physical and functional relationships between its key components. We verify the presence of an αβ2ε polymerase-clamp-exonuclease replicase complex by biochemical methods and protein-protein interaction assays in vitro and in vivo and confirm that, in addition to the polymerase activity of its α subunit, Mtb DNA pol III has two potential proofreading subunits; the α and ε subunits. During DNA replication, the presence of the β2 clamp strongly promotes the polymerization of the αβ2ε replicase and reduces its exonuclease activity. Our work provides a foundation for further research on the mechanism by which the replication machinery switches between replication and proofreading and provides an experimental platform for the selection of antimicrobials targeting DNA replication in Mtb. PMID:26822057

  10. Hepatitis B virus X protein induces RNA polymerase III-dependent gene transcription and increases cellular TATA-binding protein by activating the Ras signaling pathway.

    PubMed

    Wang, H D; Trivedi, A; Johnson, D L

    1997-12-01

    Our previous studies have shown that the hepatitis B virus protein, X, activates all three classes of RNA polymerase III (pol III)-dependent promoters by increasing the cellular level of TATA-binding protein (TBP) (H.-D. Wang et al., Mol. Cell. Biol. 15:6720-6728, 1995), a limiting transcription component (A. Trivedi et al., Mol. Cell. Biol. 16:6909-6916, 1996). We have investigated whether these X-mediated events are dependent on the activation of the Ras/Raf-1 signaling pathway. Transient expression of a dominant-negative mutant Ras gene (Ras-ala15) in a Drosophila S-2 stable cell line expressing X (X-S2), or incubation of the cells with a Ras farnesylation inhibitor, specifically blocked both the X-dependent activation of a cotransfected tRNA gene and the increase in cellular TBP levels. Transient expression of a constitutively activated form of Ras (Ras-val12) in control S2 cells produced both an increase in tRNA gene transcription and an increase in cellular TBP levels. These events are not cell type specific since X-mediated gene induction was also shown to be dependent on Ras activation in a stable rat 1A cell line expressing X. Furthermore, increases in RNA pol III-dependent gene activity and TBP levels could be restored in X-S2 cells expressing Ras-ala15 by coexpressing a constitutively activated form of Raf-1. These events are serum dependent, and when the cells are serum deprived, the X-mediated effects are augmented. Together, these results demonstrate that the X-mediated induction of RNA pol III-dependent genes and increase in TBP are both dependent on the activation of the Ras/Raf-1 signaling cascade. In addition, these studies define two new and important consequences mediated by the activation of the Ras signal transduction pathway: an increase in the central transcription factor, TBP, and the induction of RNA pol III-dependent gene activity.

  11. Tanshinone I Activates the Nrf2-Dependent Antioxidant Response and Protects Against As(III)-Induced Lung Inflammation In Vitro and In Vivo

    PubMed Central

    Tao, Shasha; Zheng, Yi; Lau, Alexandria; Jaramillo, Melba C.; Chau, Binh T.; Lantz, R. Clark; Wong, Pak K.

    2013-01-01

    Abstract Aims: The NF-E2 p45-related factor 2 (Nrf2) signaling pathway regulates the cellular antioxidant response and activation of Nrf2 has recently been shown to limit tissue damage from exposure to environmental toxicants, including As(III). In an attempt to identify improved molecular agents for systemic protection against environmental insults, we have focused on the identification of novel medicinal plant-derived Nrf2 activators. Results: Tanshinones [tanshinone I (T-I), tanshinone IIA, dihydrotanshinone, cryptotanshinone], phenanthrenequinone-based redox therapeutics derived from the medicinal herb Salvia miltiorrhiza, have been tested as experimental therapeutics for Nrf2-dependent cytoprotection. Using a dual luciferase reporter assay overexpressing wild-type or mutant Kelch-like ECH-associated protein-1 (Keap1), we demonstrate that T-I is a potent Keap1-C151-dependent Nrf2 activator that stabilizes Nrf2 by hindering its ubiquitination. In human bronchial epithelial cells exposed to As(III), T-I displays pronounced cytoprotective activity with upregulation of Nrf2-orchestrated gene expression. In Nrf2 wild-type mice, systemic administration of T-I attenuates As(III) induced inflammatory lung damage, a protective effect not observed in Nrf2 knockout mice. Innovation: Tanshinones have been identified as a novel class of Nrf2-inducers for antioxidant tissue protection in an in vivo As(III) inhalation model, that is relevant to low doses of environmental exposure. Conclusion: T-I represents a prototype Nrf2-activator that displays cytoprotective activity upon systemic administration targeting lung damage originating from environmental insults. T-I based Nrf2-directed systemic intervention may provide therapeutic benefit in protecting other organs against environmental insults. Antioxid. Redox Signal. 19, 1647–1661. PMID:23394605

  12. BIOPLUME III

    EPA Pesticide Factsheets

    BIOPLUME III is a two-dimensional finite difference model for simulating the natural attenuation of organic contaminants in groundwater due to the processes of advection, dispersion, sorption, and biodegradation.

  13. Understanding the sequential activation of Type III and Type VI Secretion Systems in Salmonella typhimurium using Boolean modeling

    PubMed Central

    2013-01-01

    Background Three pathogenicity islands, viz. SPI-1 (Salmonella pathogenicity island 1), SPI-2 (Salmonella pathogenicity island 2) and T6SS (Type VI Secretion System), present in the genome of Salmonella typhimurium have been implicated in the virulence of the pathogen. While the regulation of SPI-1 and SPI-2 (both encoding components of the Type III Secretion System - T3SS) are well understood, T6SS regulation is comparatively less studied. Interestingly, inter-connections among the regulatory elements of these three virulence determinants have also been suggested to be essential for successful infection. However, till date, an integrated view of gene regulation involving the regulators of these three secretion systems and their cross-talk is not available. Results In the current study, relevant regulatory information available from literature have been integrated into a single Boolean network, which portrays the dynamics of T3SS (SPI-1 and SPI-2) and T6SS mediated virulence. Some additional regulatory interactions involving a two-component system response regulator YfhA have also been predicted and included in the Boolean network. These predictions are aimed at deciphering the effects of osmolarity on T6SS regulation, an aspect that has been suggested in earlier studies, but the mechanism of which was hitherto unknown. Simulation of the regulatory network was able to recreate in silico the experimentally observed sequential activation of SPI-1, SPI-2 and T6SS. Conclusions The present study integrates relevant gene regulatory data (from literature and our prediction) into a single network, representing the cross-communication between T3SS (SPI-1 and SPI-2) and T6SS. This holistic view of regulatory interactions is expected to improve the current understanding of pathogenesis of S. typhimurium. PMID:24079299

  14. Sex hormone-binding globulin and antithrombin III activity in women with oral ultra-low-dose estradiol.

    PubMed

    Matsui, Sumika; Yasui, Toshiyuki; Kasai, Kana; Keyama, Kaoru; Yoshida, Kanako; Kato, Takeshi; Uemura, Hirokazu; Kuwahara, Akira; Matsuzaki, Toshiya; Irahara, Minoru

    2017-03-20

    Oral oestrogen increases the risk of venous thromboembolism (VTE) and increases production of sex hormone-binding globulin (SHBG) in a dose-dependent manner. SHBG has been suggested to be involved in venous thromboembolism. We examined the effects of oral ultra-low-dose oestradiol on circulating levels of SHBG and coagulation parameters, and we compared the effects to those of transdermal oestradiol. Twenty women received oral oestradiol (500 μg) every day (oral ultra-low-dose group) and 20 women received a transdermal patch (50 μg) as a transdermal group. In addition, the women received dydrogesterone continuously (5 mg) except for women who underwent hysterectomy. Circulating SHBG, antithrombin III (ATIII) activity, d-dimer, thrombin-antithrombin complex and plasmin-α2 plasmin inhibitor complex were measured before and 3 months after the start of treatment. SHBG was significantly increased at 3 months in the oral ultra-low-dose group, but not in the transdermal group. However, percent changes in SHBG were not significantly different between the two groups. In both groups, ATIII was significantly decreased at 3 months. In conclusion, even ultra-low-dose oestradiol orally increases circulating SHBG level. However, the magnitude of change in SHBG caused by oral ultra-low-dose oestradiol is small and is comparable to that caused by transdermal oestradiol. Impact statement Oral oestrogen replacement therapy increases production of SHBG which may be related to increase in VTE risk. However, the effect of oral ultra-low-dose oestradiol on SHBG has not been clarified. Even ultra-low-dose oestradiol orally increases circulating SHBG levels, but the magnitude of change in SHBG caused by oral ultra-low-dose oestradiol is small and is comparable to that caused by transdermal oestradiol. VTE risk in women receiving oral ultra-low-dose oestradiol may be comparable to that in women receiving transdermal oestradiol.

  15. Discovery of γ-Ray Emission from the Radio-intermediate Quasar III Zw 2: Violent Jet Activity with Intraday γ-Ray Variability

    NASA Astrophysics Data System (ADS)

    Liao, Neng-Hui; Xin, Yu-Liang; Fan, Xu-Liang; Weng, Shan-Shan; Li, Shao-Kun; Chen, Liang; Fan, Yi-Zhong

    2016-10-01

    III Zw 2 is the prototype of radio-intermediate quasars. Although there is the evidence of possessing strong jet, significant γ-ray emission has not been reported before. In this work, we carry out a detailed analysis of the latest Fermi-LAT Pass 8 data. No significant γ-ray signal has been detected in the time-averaged 7-year Fermi-LAT data of III Zw 2; however, we have identified two distinct γ-ray flares with isotropic luminosities of ˜1045 erg s-1. Multiwavelength data analysis (also including the optical photometric observations from Yunnan Observatories) are presented and the main finding is simultaneous optical and γ-ray flares of III Zw 2 appearing in 2009 November. Violent γ-ray variability with a doubling timescale of 2.5 hr was detected in another γ-ray flare in May 2010, for which the 3-hr γ-ray peak flux is ˜250 times of the average flux in 7 years. Rather similar behaviors are observed in blazars and the blazar model can reasonably reproduce the spectral energy distribution of III Zw 2 in a wide energy range, strongly suggesting that its central engine resembles that of blazars. In view of its core, which shares radio similarities with young radio sources, together with weak extended radio lobe emission, we suggest that III Zw 2 harbors a recurrent activity core and thus serves as a valuable target for investigating the fueling and triggering of the activity in radio-loud active galactic nuclei.

  16. Made in USA: Project Ethnic Heritage: Activity and Discussion Guide.

    ERIC Educational Resources Information Center

    Smith, Gary R.

    The teaching guide suggests a variety of discussion and activity strategies relating to ethnicity for secondary and continuing education students. Objectives include helping people understand the nature and significance of their own heritage as well as that of each ethnic group, and encouraging participants to realize and deal with the impact…

  17. Seeing the Sky: 100 Projects, Activities, and Explorations in Astronomy.

    ERIC Educational Resources Information Center

    Schaaf, Fred

    1990-01-01

    Fourteen astronomy activities are presented including classroom procedures and questions. Topics include different investigations of the moon, planets, stars, sunsets, light pollution, and rainbows and halos. Additional information on measurements used for observations in astronomy, and rainbow characteristics is included. (CW)

  18. Novel Ideas for Young Readers! Projects and Activities.

    ERIC Educational Resources Information Center

    Kuta, Katherine Wiesolek; Zernial, Susan

    This book offers 60 stimulating, classroom-tested activities to instill a love of literature and help young learners develop as readers, writers, and speakers. By using picture books, novels, or even nonfiction readings as starting points, the reproducible worksheets in the book can be implemented to strengthen students' entire spectrum of…

  19. Apollo 11 Facts Project: Earth Views and Crew Activities

    NASA Technical Reports Server (NTRS)

    1994-01-01

    The crewmembers of Apollo 11, Commander Neil Armstrong, Lunar Module Pilot Edwin Aldrin, Jr., and Command Module Pilot Michael Collins, are seen performing various on-orbit activities, including systems identification and Lunar Module checkout. The Earth is seen from space.

  20. Individualized Instruction in Science, Earth Space Project, Learning Activities Package.

    ERIC Educational Resources Information Center

    Kuczma, R. M.

    Learning Activity Packages (LAP) relating to the earth and space are presented for use in sampling a new type of learning for a whole year. Eighteen topics are incorporated into five units: (1) introduction to individualized learning, (2) observation versus interpretation, (3) chemistry in the space age, (4) the space age interdisciplines, and (5)…

  1. Key programmatic steps and activities for implementing the Uranium Mill Tailings Remedial Action Project. [UMTRA Project

    SciTech Connect

    Not Available

    1985-07-01

    The Uranium Mill Tailings Radiation Control Act of 1978 (UMTRCA) was enacted based upon findings by Congress that uranium mill tailings located at active and inactive hazard to the public, and that protection of the public health, safety and welfare, and the regulations of interstate commerce, require that every reasonable effort be made to provide for the stabilization, disposal, and control in a safe and environmentally sound manner of such tailings in order to prevent or minimize radon diffusion into the environment and to prevent or minimize other environmental hazards from such tailings.'' A general understanding of the steps leading to elimination of the hazards associated with designated uranium mill tailings sites, and the parties involved in that effort, are presented in this document. A representative schedule is also presented in this document to show both program sequence and activity interdependence. Those activities that have the most potential to influence program duration, because of the significant amount of additional time that may be required, include identification and selection of a suitable site, field data collection delays due to weather, actual acquisition of the designated or alternate disposal site, construction delays due to weather, and site licensing. This document provides an understanding of the steps, the sequence, the parties involved, and a representative duration of activities leading to remedial action and cleanup at the designated inactive uranium mill tailings sites. 10 refs., 5 figs., 1 tab.

  2. Activities of the Pilot Land Data System project

    NASA Technical Reports Server (NTRS)

    Sircar, J. K. (Principal Investigator)

    1985-01-01

    The University of Maryland's Remote Sensing Systems Laboratory submitted to NASA/Goddard an interim progress report on the work being conducted within its Pilot Land Data System IPLDS project. The Remote Sensing Systems Laboratory addressed the following tasks: (1) identify data types and data sources needed to describe the selected test sites in collaboration with Goddard's Hydrological Sciences Branch; (2) define the procedures necessary to access/acquire this data; (3) conduct meetings with the PLDS Systems Engineering Group to identify functional specification priorities for PLDS development; (4) assemble documentation on historical remotely sensed imagery and transfer of such information to the PLDS Data Management Group; (5) collect data identified by Goodard's Hydrological Sciences Branch for data set inventory in PLD; (6) develop a Workstation-PLDS system interface over high speed lines, (7) develop and test through a Phase 1 demonstration of a micro workstation to access PLDS; and (8) establish interdepartmental agreement of development of computer link for electronic access of water resources data from USGS.

  3. Recovery Efficiency Test Project: Phase 1, Activity report

    SciTech Connect

    Overbey, W.K. Jr.; Carden, R.S.; Kirr, J.N.

    1987-04-01

    The recovery Efficiency Test well project addressed a number of technical issues. The primary objective was to determine the increased efficiency gas recovery of a long horizontal wellbore over that of a vertical wellbore and, more specifically, what improvements can be expected from inducing multiple hydraulic fractures from such a wellbore. BDM corporation located, planned, and drilled a long radius turn horizontal well in the Devonian shale Lower Huron section in Wayne County, West Virginia, demonstrating that state-of-the-art technology is capable of drilling such wells. BDM successfully tested drilling, coring, and logging in a horizontal well using air as the circulating medium; conducted reservoir modeling studies to protect flow rates and reserves in advance of drilling operations; observed two phase flow conditions in the wellbore not observed previously; cored a fracture zone which produced gas; observed that fractures in the core and the wellbore were not systematically spaced (varied from 5 to 68 feet in different parts of the wellbore); observed that highest gas show rates reported by the mud logger corresponded to zone with lowest fracture spacing (five feet) or high fracture frequency. Four and one-half inch casting was successfully installed in the borehole and was equipped to isolate the horizontal section into eight (8) zones for future testing and stimulation operations. 6 refs., 48 figs., 10 tabs.

  4. Recovery efficiency test project, Phase 2 activity report

    SciTech Connect

    Overbey, W.K. Jr.; Salamy, S.P.; Locke, C.D.

    1989-02-01

    The Recovery Efficiency Test well project addressed a number of technical issues. The primary objective was to determine the increased efficiency of gas recovery of a long horizontal wellbore over that of a vertical wellbore and, more specifically, what improvements can be expected from inducing multiple hydraulic fractures from such a wellbore. This volume contains appendices for: (1) supporting material and procedures for data frac'' stimulation of zone 6 using nitrogen and nitrogen foam; (2) supporting material and procedures for stimulation no. 1 nitrogen gas frac on zone no. 1; (3) supporting material and procedures for stimulation no. 2 in zone no. 1 using liquid CO{sub 2}; (4) supporting material and procedures for frac no. 3 on zone no.1 using nitrogen foam and proppant; (5) supporting material and procedures for stimulation no. 4 in zones 2--3 and 4 using nitrogen foam and proppant; (6) supporting materials and procedures for stimulation no. 5 in zones 5 and 8; and (7) fracture diagnostics reports and supporting materials.

  5. Proposed scientific activities for the Salton Sea Scientific Drilling Project

    SciTech Connect

    Not Available

    1984-05-01

    The Salton Sea Scientific Drilling Project (SSSDP) has been organized for the purpose of investigating a hydrothermal system at depths and temperatures greater than has been done before. Plans are to deepen an existing well or to drill a new well for research purposes for which temperatures of 300/sup 0/C will be reached at a depth of less than 3.7 km and then deepen that well a further 1.8 km. This report recounts the Congressional history of the appropriation to drill the hole and other history through March 1984, gives a review of the literature on the Salton Sea Geothermal Field and its relationship to other geothermal systems of the Salton Trough, and describes a comprehensive series of investigations that have been proposed either in the well or in conjunction with the SSSDP. Investigations in geophysics, geochemistry and petrology, tectonics and rock mechanics, and geohydrology are given. A tabulation is given of current commercial and state-of-the-art downhole tools and their pressure, temperature, and minimum hole size limitations.

  6. Recovery Efficiency Test Project Phase 2 activity report, Volume 1

    SciTech Connect

    Overbey, W.K. Jr.; Salamy, S.P.; Locke, C.D.

    1989-02-01

    The purpose of Phase II operations of the Recovery Efficiency Test Project is to enhance the natural production of the well and evaluate the relative improvement as a function of the type of stimulation conducted. Another purpose is to compare the stimulated production performance of the horizontal well with vertical wells in the field. The objectives considered for Phase II operations and plans were: (1) Develop a rationale for a systematic approach to designing stimulations for the well. (2) Conduct a series of stimulations designed to optimize the fluids, injection rates, proppant volumes and general approach to stimulating a horizontal well with similar geologic conditions. (3) Develop and test a method or methods for determining the geometry of stimulation-induced fractures. (4) Conduct tests and analyze the results to determine the efficiency of stimulation operations. The technical approach pursued in developing plans to accomplish three objectives was to: (1) Review the data needs for all objectives and obtain that data first. (2) Identify the operating geologic, geomechanical, and reservoir parameters that need additional clarification or definition. (3) Investigate existing models which could be used to plan or evaluate stimulation on the well and the reservoir. (4) Plan for analysis and verification of models and approaches.

  7. Synthesis, structure, DNA/protein binding, and cytotoxic activity of a rhodium(III) complex with 2,6-bis(2-benzimidazolyl)pyridine.

    PubMed

    Esteghamat-Panah, Roya; Hadadzadeh, Hassan; Farrokhpour, Hossein; Simpson, Jim; Abdolmaleki, Amir; Abyar, Fatemeh

    2017-02-15

    A new mononuclear rhodium(III) complex, [Rh(bzimpy)Cl3] (bzimpy = 2,6-bis(2-benzimidazolyl)pyridine), was synthesized and characterized by elemental analysis and spectroscopic methods. The molecular structure of the complex was confirmed by single-crystal X-ray crystallography. The interaction of the complex with fish sperm DNA (FS-DNA) was investigated by UV spectroscopy, emission titration, and viscosity measurement in order to evaluate the possible DNA-binding mode and to calculate the corresponding DNA-binding constant. The results reveal that the Rh(III) complex interacts with DNA through groove binding mode with a binding affinity on the order of 10(4). In addition, the binding of the Rh(III) complex to bovine serum albumin (BSA) was monitored by UV-Vis and fluorescence emission spectroscopy at different temperatures. The mechanism of the complex interaction was found to be static quenching. The thermodynamic parameters (ΔH, ΔS, and ΔG) obtained from the fluorescence spectroscopy data show that van der Waals interactions and hydrogen bonds play a major role in the binding of the Rh(III) complex to BSA. For the comparison of the DNA- and BSA-binding affinities of the free bzimpy ligand with its Rh(III) complex, the absorbance titration and fluorescence quenching experiments of the free bzimpy ligand with DNA and BSA were carried out. Competitive experiments using eosin Y and ibuprofen as site markers indicated that the complex was mainly located in the hydrophobic cavity of site I of the protein. These experimental results were confirmed by the results of molecular docking. Finally, the in vitro cytotoxicity properties of the Rh(III) complex against the MCF-7, K562, and HT-29 cell lines were evaluated and compared with those of the free ligand (bzimpy). It was found that the complexation process improved the anticancer activity significantly.

  8. The MicroActive project: automatic detection of disease-related molecular cell activity

    NASA Astrophysics Data System (ADS)

    Furuberg, Liv; Mielnik, Michal; Johansen, Ib-Rune; Voitel, Jörg; Gulliksen, Anja; Solli, Lars; Karlsen, Frank; Bayer, Tobias; Schönfeld, Friedhelm; Drese, Klaus; Keegan, Helen; Martin, Cara; O'Leary, John; Riegger, Lutz; Koltay, Peter

    2007-05-01

    The aim of the MicroActive project is to develop an instrument for molecular diagnostics. The instrument will first be tested for patient screening for a group of viruses causing cervical cancer. Two disposable polymer chips with reagents stored on-chip will be inserted into the instrument for each patient sample. The first chip performs sample preparation of the epithelial cervical cells while mRNA amplification and fluorescent detection takes place in the second chip. More than 10 different virus markers will be analysed in one chip. We report results on sub-functions of the amplification chip. The sample is split into smaller droplets, and the droplets move in parallel channels containing different dried reagents for the different analyses. We report experimental results on parallel droplet movement control using one external pump only, combined with hydrophobic valves. Valve burst pressures are controlled by geometry. We show droplet control using valves with burst pressures between 800 and 4500 Pa. We also monitored the re-hydration times for two necessary dried reagents. After sample insertion, uniform concentration of the reagents in the droplet was reached after respectively 60 s and 10 min. These times are acceptable for successful amplification. Finally we have shown positive amplification of HPV type 16 using dried enzymes stored in micro chambers.

  9. Structure of solvation water around the active and inactive regions of a type III antifreeze protein and its mutants of lowered activity

    NASA Astrophysics Data System (ADS)

    Grabowska, Joanna; Kuffel, Anna; Zielkiewicz, Jan

    2016-08-01

    Water molecules from the solvation shell of the ice-binding surface are considered important for the antifreeze proteins to perform their function properly. Herein, we discuss the problem whether the extent of changes of the mean properties of solvation water can be connected with the antifreeze activity of the protein. To this aim, the structure of solvation water of a type III antifreeze protein from Macrozoarces americanus (eel pout) is investigated. A wild type of the protein is used, along with its three mutants, with antifreeze activities equal to 54% or 10% of the activity of the native form. The solvation water of the ice-binding surface and the rest of the protein are analyzed separately. To characterize the structure of solvation shell, parameters describing radial and angular characteristics of the mutual arrangement of the molecules were employed. They take into account short-distance (first hydration shell) or long-distance (two solvation shells) effects. The obtained results and the comparison with the results obtained previously for a hyperactive antifreeze protein from Choristoneura fumiferana lead to the conclusion that the structure and amino acid composition of the active region of the protein evolved to achieve two goals. The first one is the modification of the properties of the solvation water. The second one is the geometrical adjustment of the protein surface to the specific crystallographic plane of ice. Both of these goals have to be achieved simultaneously in order for the protein to perform its function properly. However, they seem to be independent from one another in a sense that very small antifreeze activity does not imply that properties of water become different from the ones observed for the wild type. The proteins with significantly lower activity still modify the mean properties of solvation water in a right direction, in spite of the fact that the accuracy of the geometrical match with the ice lattice is lost because of the

  10. Water-soluble oxoglaucine-Y(III), Dy(III) complexes: in vitro and in vivo anticancer activities by triggering DNA damage, leading to S phase arrest and apoptosis.

    PubMed

    Wei, Jian-Hua; Chen, Zhen-Feng; Qin, Jiao-Lan; Liu, Yan-Cheng; Li, Zhu-Quan; Khan, Taj-Malook; Wang, Meng; Jiang, Yan-Hua; Shen, Wen-Ying; Liang, Hong

    2015-07-07

    Complexes of yttrium(III) and dysprosium(III) with the traditional Chinese medicine active ingredient oxoglaucine (OG), namely [Y(OG)2(NO3)3]·CH3OH (1) and [Dy(OG)2(NO3)3]·H2O (2), were synthesized and characterized by elemental analysis, IR, ESI-MS, (1)H and (13)C NMR as well as single-crystal X-ray diffraction analysis. In vitro the complexes exhibited higher anticancer activity than the free ligand OG against the tested cancer cell lines. Among the tested cell lines, HepG2 is the most sensitive to the complexes. Complex 2 can trigger DNA damage in HepG2 cells, resulting in cell cycle arrest in the S phase and leading to cell apoptosis. The S phase cell-cycle arrest is caused via the ATM (ataxia-telangiectasia mutated)-Chk2-Cdc25A pathway. Chk2 is phosphorylated and activated in an ATM-dependent manner. It, in turn, phosphorylates Cdc25A phosphatise on serine124, causing the inactivation of Cdc25A in ubiquitin-mediated proteolytic degradation. The cyclin-Cdk complexes of the S phase could also be inhibited by limited supply of cyclins A and E. This irreversible cell cycle arrest process ultimately induces mitochondria-involved apoptotic cell death via the activation of Bcl-2 protein. Complex e2 ffectively inhibited tumour growth in the BEL-7402 xenograft mouse model and exhibited higher safety in vivo than cisplatin.

  11. Pea formaldehyde-active class III alcohol dehydrogenase: common derivation of the plant and animal forms but not of the corresponding ethanol-active forms (classes I and P).

    PubMed Central

    Shafqat, J; El-Ahmad, M; Danielsson, O; Martínez, M C; Persson, B; Parés, X; Jornvall, H

    1996-01-01

    A plant class III alcohol dehydrogenase (or glutathione-dependent formaldehyde dehydrogenase) has been characterized. The enzyme is a typical class III member with enzymatic parameters and substrate specificity closely related to those of already established animal forms. Km values with the pea enzyme are 6.5 microM for NAD+, 2 microM for S-hydroxymethylglutathione, and 840 microM for octanol versus 9, 4, and 1200 microM, respectively, with the human enzyme. Structurally, the pea/human class III enzymes are closely related, exhibiting a residue identity of 69% and with only 3 of 23 residues differing among those often considered in substrate and coenzyme binding. In contrast, the corresponding ethanol-active enzymes, the long-known human liver and pea alcohol dehydrogenases, differ more (47% residue identities) and are also in functionally important active site segments, with 12 of the 23 positions exchanged, including no less than 7 at the usually much conserved coenzyme-binding segment. These differences affect functionally important residues that are often class-distinguishing, such as those at positions 48, 51, and 115, where the plant ethanol-active forms resemble class III (Thr, Tyr, and Arg, respectively) rather than the animal ethanol-active class I forms (typically Ser, His, and Asp, respectively). Calculations of phylogenetic trees support the conclusions from functional residues in subgrouping plant ethanol-active dehydrogenases and the animal ethanol-active enzymes (class I) as separate descendants from the class III line. It appears that the classical plant alcohol dehydrogenases (now called class P) have a duplicatory origin separate from that of the animal class I enzymes and therefore a paralogous relationship with functional convergence of their alcohol substrate specificity. Combined, the results establish the conserved nature of class III also in plants, and contribute to the molecular and functional understanding of alcohol dehydrogenases by

  12. Multifunctionality in bimetallic Ln(III)[W(V)(CN)8]3- (Ln = Gd, Nd) coordination helices: optical activity, luminescence, and magnetic coupling.

    PubMed

    Chorazy, Szymon; Nakabayashi, Koji; Arczynski, Mirosław; Pełka, Robert; Ohkoshi, Shin-ichi; Sieklucka, Barbara

    2014-06-02

    Two chiral luminescent derivatives of pyridine bis(oxazoline) (Pybox), (SS/RR)-iPr-Pybox (2,6-bis[4-isopropyl-2-oxazolin-2-yl]pyridine) and (SRSR/RSRS)-Ind-Pybox (2,6-bis[8H-indeno[1,2-d]oxazolin-2-yl]pyridine), have been combined with lanthanide ions (Gd(3+), Nd(3+)) and octacyanotungstate(V) metalloligand to afford a remarkable series of eight bimetallic CN(-)-bridged coordination chains: {[Ln(III)(SS/RR-iPr-Pybox)(dmf)4]3[W(V)(CN)8]3}n ⋅dmf⋅4 H2O (Ln = Gd, 1-SS and 1-RR; Ln = Nd, 2-SS and 2-RR) and {[Ln(III)(SRSR/RSRS-Ind-Pybox)(dmf)4][W(V)(CN)8]}n⋅5 MeCN⋅4 MeOH (Ln = Gd, 3-SRSR and 3-RSRS; Ln = Nd, 4-SRSR and 4-RSRS). These materials display enantiopure structural helicity, which results in strong optical activity in the range 200-450 nm, as confirmed by natural circular dichroism (NCD) spectra and the corresponding UV/Vis absorption spectra. Under irradiation with UV light, the Gd(III)-W(V) chains show dominant ligand-based red phosphorescence, with λmax ≈660 nm for 1-(SS/RR) and 680 nm for 3-(SRSR/RSRS). The Nd(III)-W(V) chains, 2-(SS/RR) and 4-(SRSR/RSRS), exhibit near-infrared luminescence with sharp lines at 986, 1066, and 1340 nm derived from intra-f (4)F3/2 → (4)I9/2,11/2,13/2 transitions of the Nd(III) centers. This emission is realized through efficient ligand-to-metal energy transfer from the Pybox derivative to the lanthanide ion. Due to the presence of paramagnetic lanthanide(III) and [W(V)(CN)8](3-) moieties connected by cyanide bridges, 1-(SS/RR) and 3-(SRSR/RSRS) are ferrimagnetic spin chains originating from antiferromagnetic coupling between Gd(III) (SGd = 7/2) and W(V) (SW = 1/2) centers with J1-(SS) = -0.96(1) cm(-1), J1-(RR) =-0.95(1) cm(-1), J3-(SRSR) = -0.91(1) cm(-1), and J3-(RSRS) =-0.94(1) cm(-1). 2-(SS/RR) and 4-(SRSR/RSRS) display ferromagnetic coupling within their Nd(III)-NC-W(V) linkages.

  13. LUSI LAB: a multidisciplinary project in a natural active laboratory

    NASA Astrophysics Data System (ADS)

    Mazzini, Adriano; Lusi Lab Team

    2016-04-01

    The 29th of May 2006 several gas and mud eruption sites suddenly appeared along a strike-slip fault (Watukosek fault system) in the NE of Java, Indonesia. The eruption occurred almost two days after a 6.3 M earthquake striking the island of Java. Within weeks several villages were submerged by boiling mud. The most prominent eruption site was named Lusi. To date Lusi is still active. This disaster has forced 50.000 people to be evacuated and an area of ~7 km2 is covered by mud. The social impact of the eruption and its spectacular dimensions still attract the attention of international media reporting on the "largest mud eruption site on Earth". LUSI LAB (ERC grant n° 308126) focuses on five main aspects in order to complete a comprehensive regional investigation of this impressive event: 1) sampling and monitoring the active Lusi eruption site; 2) monitoring and sampling the neighbouring volcanic arc; 3) monitoring the local micro-seismicity and its relationship with regional seismicity; 4) monitoring the fault system originating from the volcanic arc, crossing Lusi and extending to the NE of Java island; 5) numerical modelling of Lusi activity and the strike-slip/magmatic complex system. We completed several field expeditions. Our studies investigated the mechanisms of reactivation of the Watukosek fault system that crosses Lusi locality and continues to the NE of Java. Results show that after the 27-05-2009 earthquake it was activated the lateral movement of this strike-slip system resulting in these several aligned eruptions sites including Lusi. Further, our geochemical studies of the erupted fluids reveal a mantle signature and point to a connection with the neighboring Arjuno-Welirang volcanic complex indicating that Lusi is a sedimentary hosted geothermal system. We have designed, developed and constructed the Lusi drone. This is a remote controlled hexacopter developed and assembled in order to complete multidisciplinary studies in extreme and

  14. Apollo 11 Facts Project [Pre-Launch Activities and Launch

    NASA Technical Reports Server (NTRS)

    1994-01-01

    The crewmembers of Apollo 11, Commander Neil A. Armstrong, Command Module Pilot Michael Collins, and Lunar Module Pilot Edwin E. Aldrin, Jr., are seen during various stages of preparation for the launch of Apollo 11, including suitup, breakfast, and boarding the spacecraft. They are also seen during mission training, including preparation for extravehicular activity on the surface of the Moon. The launch of Apollo 11 is shown. The ground support crew is also seen as they wait for the spacecraft to approach the Moon.

  15. Design requirements document for project W-520, immobilized low-activity waste disposal

    SciTech Connect

    Ashworth, S.C.

    1998-08-06

    This design requirements document (DRD) identifies the functions that must be performed to accept, handle, and dispose of the immobilized low-activity waste (ILAW) produced by the Tank Waste Remediation System (TWRS) private treatment contractors and close the facility. It identifies the requirements that are associated with those functions and that must be met. The functional and performance requirements in this document provide the basis for the conceptual design of the Tank Waste Remediation System Immobilized Low-Activity Waste disposal facility project (W-520) and provides traceability from the program-level requirements to the project design activity.

  16. Individualized Instruction in Science, Earth-Space Project, Self-Directed Activities.

    ERIC Educational Resources Information Center

    Kuczma, R. M.

    As a supplement to Learning Activity Packages (LAP) of the earth-space project, this manual presents self-directed activities especially designed for individualized instruction. Besides an introduction to LAP characteristics, sets of instructions are given in connection with the metric system, the earth's dimensions, indirect evidence for atomic…

  17. A Student Activity for the James Bay Hydro Project. The Geography Teacher.

    ERIC Educational Resources Information Center

    Green-Milberg, Patricia

    1999-01-01

    Provides activities for grades 6 to 8 that will promote student awareness and understanding of the use of hydropower in Canada, the benefits and drawbacks to hydropower, and also the drawbacks of electricity transmission lines. Explains that the activities focus on the James Bay Hydro Project in Canada. (CMK)

  18. Newspaper Content Analysis in Evaluation of a Community-Based Participatory Project to Increase Physical Activity

    ERIC Educational Resources Information Center

    Granner, Michelle L.; Sharpe, Patricia A.; Burroughs, Ericka L.; Fields, Regina; Hallenbeck, Joyce

    2010-01-01

    This study conducted a newspaper content analysis as part of an evaluation of a community-based participatory research project focused on increasing physical activity through policy and environmental changes, which included activities related to media advocacy and media-based community education. Daily papers (May 2003 to December 2005) from both…

  19. SOIL AND WATER CONSERVATION PROJECTS AND ACTIVITIES, A GUIDE FOR 4-H CLUB LEADERS.

    ERIC Educational Resources Information Center

    FOSTER, ALBERT B.; FOX, ADRIAN C.

    THIS PUBLICATION WAS PREPARED BY THE SOIL CONSERVATION SERVICE FOR USE WITH YOUTH GROUPS. VARIOUS ACTIVITIES AND PROJECTS ARE PRESENTED WHICH CAN BE USED TO DEVELOP CONCEPTS ABOUT SOIL AND WATER CONSERVATION. IN ORDER TO SIMPLIFY THE PROCESS OF DEMONSTRATING THESE ACTIVITIES, MANY OF THE CONCEPTS ARE PICTORIALLY ILLUSTRATED. THE ACTIVITIES…

  20. 12 Years of Action Learning at EM Normandie: Monitored Field Projects as Regular Pedagogical Activities

    ERIC Educational Resources Information Center

    Anger, Sophie Gay; Hachard, Virginie

    2011-01-01

    The Master Grande Ecole curriculum at EM Normandie School is organized around junior consulting projects and real problem solving activities aiming at bridging the gap between classroom knowledge and professional competencies. Since the 90's, students are involved in regular consulting activities for local and national companies following the…

  1. Focused Campaign Increases Activity among Participants in "Nature's Notebook," a Citizen Science Project

    ERIC Educational Resources Information Center

    Crimmins, Theresa M.; Weltzin, Jake F.; Rosemartin, Alyssa H.; Surina, Echo M.; Marsh, Lee; Denny, Ellen G.

    2014-01-01

    Science projects, which engage non-professional scientists in one or more stages of scientific research, have been gaining popularity; yet maintaining participants' activity level over time remains a challenge. The objective of this study was to evaluate the potential for a short-term, focused campaign to increase participant activity in a…

  2. A "turn-on" and label-free fluorescent assay for the rapid detection of exonuclease III activity based on Tb(3+)-induced G-quadruplex conjugates.

    PubMed

    Yang, WeiJuan; Ruan, YaJuan; Wu, WeiHua; Chen, PingPing; Xu, LiangJun; Fu, FengFu

    2014-07-01

    A "turn-on" and label-free fluorescent assay for the specific, rapid, and sensitive detection of 3' → 5' exonuclease III activity is reported in this study. The assay is based on the Tb(3+)-promoted G-quadruplex, which lead to the enhancement of Tb(3+) fluorescence due to the energy transfer from guanines. The proposed assay is highly simple, rapid, and cost-effective, and does not require sophisticated experimental techniques such as gel-based equipment or radioactive labels. It can be used for the rapid detection of exonuclease III activity with a detection limit of 0.8 U and a RSD (n = 6) <5 %. Notably, no dye was covalently conjugated to the DNA strands, which offers the advantages of low-cost and being interference-free.

  3. Nowcasting and forecasting of lightning activity: the Talos project.

    NASA Astrophysics Data System (ADS)

    Lagouvardos, Kostas; Kotroni, Vassiliki; Kazadzis, Stelios; Giannaros, Theodore; Karagiannidis, Athanassios; Galanaki, Elissavet; Proestakis, Emmanouil

    2015-04-01

    Thunder And Lightning Observing System (TALOS) is a research program funded by the Greek Ministry of Education with the aim to promote excellence in the field of lightning meteorology. The study focuses on exploring the real-time observations provided by the ZEUS lightning detection system, operated by the National Observatory of Athens since 2005, as well as the 10-year long database of the same system. More precisely the main research issues explored are: - lightning climatology over the Mediterranean focusing on lightning spatial and temporal distribution, on the relation of lightning with topographical features and instability and on the importance of aerosols in lightning initiation and enhancement. - nowcasting of lightning activity over Greece, with emphasis on the operational aspects of this endeavour. The nowcasting tool is based on the use of lightning data complemented by high-time resolution METEOSAT imagery. - forecasting of lightning activity over Greece based on the use of WRF numerical weather prediction model. - assimilation of lightning with the aim to improve the model precipitation forecast skill. In the frame of this presentation the main findings of each of the aforementioned issues are highlighted.

  4. Coal Ash Behavior in Reducing Environments (CABRE) III Year 6 - Activity 1.10 - Development of a National Center for Hydrogen

    SciTech Connect

    Stanislowski, Joshua; Azenkeng, Alexander; McCollor, Donald; Galbreath, Kevin; Jensen, Robert; Lahr, Brent

    2012-03-31

    The Energy & Environmental Research Center (EERC) has been conducting research on gasification for six decades. One of the objectives of this gasification research has been to maximize carbon conversion and the water–gas shift process for optimal hydrogen production and syngas quality. This research focus and experience were a perfect fit for the National Center for Hydrogen Technology ® (NCHT®) Program at the EERC for improving all aspects of coal gasification, which ultimately aids in the production and purification of hydrogen. A consortia project was developed under the NCHT Program to develop an improved predictive model for ash formation and deposition under the project entitled “Coal Ash Behavior in Reducing Environments (CABRE) III: Development of the CABRE III Model.” The computer-based program is now applicable to the modeling of coal and ash behavior in both entrained-flow and fluidized-bed gasification systems to aid in overall gasification efficiency. This model represents a significant improvement over the CABRE II model and runs on a Microsoft Windows PC platform. The major achievements of the CABRE III model are partitioning of inorganic transformations between various phases for specific gas cleanup equipment; slag property predictions, including standard temperature–viscosity curves and slag flow and thickness; deposition rates in gasification cleanup equipment; provision for composition analysis for all input and output streams across all process equipment, including major elements and trace elements of interest; composition analysis of deposit streams for various deposit zones, including direct condensation on equipment surfaces (Zone A), homogeneous particulate deposition (Zone B), and entrained fly ash deposition (Zone C); and physical removal of ash in cyclones based on D50 cut points. Another new feature of the CABRE III model is a user-friendly interface and detailed reports that are easily exportable into Word documents, Excel

  5. Iridium-mediated C-S bond activation and transformation: organoiridium(III) thioether, thiolato, sulfinato and thiyl radical compounds. Synthesis, mechanistic, spectral, electrochemical and theoretical aspects.

    PubMed

    Das, Ujjwal; Ghorui, Tapas; Adhikari, Basab; Roy, Sima; Pramanik, Shuvam; Pramanik, Kausikisankar

    2015-05-14

    An attractive methodology, single-electron transfer (SET) reductive cleavage of the C-S bond mediated by a metal in the presence of the external stimuli PPh3, has been applied to the kinetically inert IrCl3 in order to synthesize the thiolato complex [Ir(III)(L(S))Cl(PPh3)2] 3 from precursor thioether complexes [Ir(III)(L(SR))Cl2(PPh3)] (R = alkyl) 2. The aforesaid cleavage process in association with (arene)C-H activation furnishes a new class of organosulfur compounds of iridium(III). The thiolato chelate 3 displays a reversible oxidative wave at 0.75 V vs. Ag/AgCl signifying its remarkable nucleophilic character. The high electron density on the thiolato-S vis-à-vis superior nucleophilicity can be envisaged through the formation of a number of S-centered derivatives. This observation has been corroborated with the nature of HOMO in 3, which assumes 49% of S(3p). Notably, the facile oxidative nature of 3 makes it an apposite precursor for metal-stabilized thiyl radical species. Indeed, iridium(III)-stabilized 3˙(+) can be generated by chemical/electrochemical means. The axial EPR spectra with g ∼ 2.0 along with theoretical analysis of SOMO (S(3p) 24% + Ph(π) 43% + d(yz) 15%) and spin density (ρ(S) = +0.543, ρ(Ph) = +0.315, ρ(Ir) = +0.151) of one-electron oxidized 3˙(+) validate the iridium-stabilized thiyl radical description. This observation suggests that the CNS coordination mode in thiophenolato complex 3 is redox-active. Complex 3 is very prone to S-centered oxidation under normal aerobic conditions to yield metallosulfoxide [Ir(III)(L(SO2))Cl(PPh3)2] 4. The enhanced nucleophilicity of thiolato-S can also be manifested via the smooth S-C bond making process with alkyl halides (R'X, R' = Me and allyl; X = Br, I) and subsequent formation of thioether complexes of type [Ir(III)(L(SR'))ClX(PPh3)] 5. The organosulfur compounds of iridium(III) exhibit rich spectral properties including luminescence and the origin of these transitions is scrutinized with

  6. Hermes III endpoint energy calculation from photonuclear activation of 197Au and 58Ni foils

    SciTech Connect

    Parzyck, Christopher Thomas

    2014-09-01

    A new process has been developed to characterize the endpoint energy of HERMES III on a shot-to-shot basis using standard dosimetry tools from the Sandia Radiation Measurements Laboratory. Photonuclear activation readings from nickel and gold foils are used in conjunction with calcium fluoride thermoluminescent dosimeters to derive estimated electron endpoint energies for a series of HERMES shots. The results are reasonably consistent with the expected endpoint voltages on those shots.

  7. An unexpected cobalt(III) complex containing a Schiff base ligand: Synthesis, crystal structure, spectroscopic behavior, electrochemical property and SOD-like activity.

    PubMed

    Chai, Lan-Qin; Huang, Jiao-Jiao; Zhang, Hong-Song; Zhang, Yu-Li; Zhang, Jian-Yu; Li, Yao-Xin

    2014-10-15

    An unexpected mononuclear Co(III) complex, [Co(L2)2·(CH3COO)]·CH3OH (HL2=1-(2-{[(E)-3,5-dichloro-2-hydroxybenzylidene]amino}phenyl)ethanone oxime), has been synthesized via complexation of Co(II) acetate tetrahydrate with HL1 originally. The plausible reaction mechanism for the formation of quinazoline-type ligand was proposed. HL1 and its corresponding Co(III) complex were characterized by IR, as well as by elemental analysis and UV-vis spectroscopy. The crystal structure of the complex has been determined by single-crystal X-ray diffraction. Each complex links two other molecules into an infinite 1-D chain via intermolecular hydrogen bonding interactions. Moreover, the electrochemical properties of the cobalt(III) complex were studied by cyclic voltammetry and X-ray photoelectron spectrum (XPS). In addition, superoxide dismutase-like activities of HL1 and Co(III) complex were also investigated.

  8. A Single RNaseIII Domain Protein from Entamoeba histolytica Has dsRNA Cleavage Activity and Can Help Mediate RNAi Gene Silencing in a Heterologous System.

    PubMed

    Pompey, Justine M; Foda, Bardees; Singh, Upinder

    2015-01-01

    Dicer enzymes process double-stranded RNA (dsRNA) into small RNAs that target gene silencing through the RNA interference (RNAi) pathway. Dicer enzymes are complex, multi-domain RNaseIII proteins, however structural minimalism of this protein has recently emerged in parasitic and fungal systems. The most minimal Dicer, Saccharomyces castellii Dicer1, has a single RNaseIII domain and two double stranded RNA binding domains. In the protozoan parasite Entamoeba histolytica 27nt small RNAs are abundant and mediate silencing, yet no canonical Dicer enzyme has been identified. Although EhRNaseIII does not exhibit robust dsRNA cleavage in vitro, it can process dsRNA in the RNAi-negative background of Saccharomyces cerevisiae, and in conjunction with S. castellii Argonaute1 can partially reconstitute the RNAi pathway. Thus, although EhRNaseIII lacks the domain architecture of canonical or minimal Dicer enzymes, it has dsRNA processing activity that contributes to gene silencing via RNAi. Our data advance the understanding of small RNA biogenesis in Entamoeba as well as broaden the spectrum of non-canonical Dicer enzymes that contribute to the RNAi pathway.

  9. A Single RNaseIII Domain Protein from Entamoeba histolytica Has dsRNA Cleavage Activity and Can Help Mediate RNAi Gene Silencing in a Heterologous System

    PubMed Central

    Singh, Upinder

    2015-01-01

    Dicer enzymes process double-stranded RNA (dsRNA) into small RNAs that target gene silencing through the RNA interference (RNAi) pathway. Dicer enzymes are complex, multi-domain RNaseIII proteins, however structural minimalism of this protein has recently emerged in parasitic and fungal systems. The most minimal Dicer, Saccharomyces castellii Dicer1, has a single RNaseIII domain and two double stranded RNA binding domains. In the protozoan parasite Entamoeba histolytica 27nt small RNAs are abundant and mediate silencing, yet no canonical Dicer enzyme has been identified. Although EhRNaseIII does not exhibit robust dsRNA cleavage in vitro, it can process dsRNA in the RNAi-negative background of Saccharomyces cerevisiae, and in conjunction with S. castellii Argonaute1 can partially reconstitute the RNAi pathway. Thus, although EhRNaseIII lacks the domain architecture of canonical or minimal Dicer enzymes, it has dsRNA processing activity that contributes to gene silencing via RNAi. Our data advance the understanding of small RNA biogenesis in Entamoeba as well as broaden the spectrum of non-canonical Dicer enzymes that contribute to the RNAi pathway. PMID:26230096

  10. The activation patterns of embryonic chick motoneurones projecting to inappropriate muscles.

    PubMed Central

    Landmesser, L T; O'Donovan, M J

    1984-01-01

    Chick lumbosacral motoneurones were caused to innervate foreign muscles by surgically rotating or shifting the limb bud about the anterior-posterior axis in stage 17-18 embryos. The activation pattern of such wrongly projecting motoneurones was assessed at stages 35-38 by recording electromyographic activity from muscles in an isolated spinal cord/hind limb preparation. Muscle activity was classed as flexor- or extensor-like according to the characteristics of the patterned sequence of bursts elicited by a single shock to the thoracic cord. Wrongly projecting motoneurones did not have their activation pattern altered to one appropriate for the muscle innervated; therefore in some cases a particular muscle was activated with a pattern similar to its original one, and in other cases in an opposite manner. Mixed flexor-extensor-like activation of a single muscle was, however, rare. The identity of motoneurones projecting to a muscle was determined by their cord location following retrograde labelling with horseradish peroxidase. This allowed us to conclude that motoneurones could develop their normal pattern of activation even when projecting to foreign muscles. It is concluded that the cord circuits (presumably composed of local interneurones responsible for the activation of motoneurones in the isolated cord preparation are not altered by retrograde influences from the muscle. Wrongly projecting motoneurones, which were maintained throughout the normal cell death period, were activated during spontaneous embryonic movements, and in many cases were found to have a behaviourally inappropriate activation pattern. These observations are discussed in relation to proposed mechanisms by which developmental errors in connectivity are corrected. Images Fig. 1 PMID:6707957

  11. The activation patterns of embryonic chick motoneurones projecting to inappropriate muscles.

    PubMed

    Landmesser, L T; O'Donovan, M J

    1984-02-01

    Chick lumbosacral motoneurones were caused to innervate foreign muscles by surgically rotating or shifting the limb bud about the anterior-posterior axis in stage 17-18 embryos. The activation pattern of such wrongly projecting motoneurones was assessed at stages 35-38 by recording electromyographic activity from muscles in an isolated spinal cord/hind limb preparation. Muscle activity was classed as flexor- or extensor-like according to the characteristics of the patterned sequence of bursts elicited by a single shock to the thoracic cord. Wrongly projecting motoneurones did not have their activation pattern altered to one appropriate for the muscle innervated; therefore in some cases a particular muscle was activated with a pattern similar to its original one, and in other cases in an opposite manner. Mixed flexor-extensor-like activation of a single muscle was, however, rare. The identity of motoneurones projecting to a muscle was determined by their cord location following retrograde labelling with horseradish peroxidase. This allowed us to conclude that motoneurones could develop their normal pattern of activation even when projecting to foreign muscles. It is concluded that the cord circuits (presumably composed of local interneurones responsible for the activation of motoneurones in the isolated cord preparation are not altered by retrograde influences from the muscle. Wrongly projecting motoneurones, which were maintained throughout the normal cell death period, were activated during spontaneous embryonic movements, and in many cases were found to have a behaviourally inappropriate activation pattern. These observations are discussed in relation to proposed mechanisms by which developmental errors in connectivity are corrected.

  12. Experimental determination of water activity for binary aqueous cerium(III) ionic solutions: application to an assessment of the predictive capability of the binding mean spherical approximation model.

    PubMed

    Ruas, Alexandre; Simonin, Jean-Pierre; Turq, Pierre; Moisy, Philippe

    2005-12-08

    This work is aimed at a description of the thermodynamic properties of actinide salt solutions at high concentration. The predictive capability of the binding mean spherical approximation (BIMSA) theory to describe the thermodynamic properties of electrolytes is assessed in the case of aqueous solutions of lanthanide(III) nitrate and chloride salts. Osmotic coefficients of cerium(III) nitrate and chloride were calculated from other lanthanide(III) salts properties. In parallel, concentrated binary solutions of cerium nitrate were prepared in order to measure experimentally its water activity and density as a function of concentration, at 25 degrees C. Water activities of several binary solutions of cerium chloride were also measured to check existing data on this salt. Then, the properties of cerium chloride and cerium nitrate solutions were compared within the BIMSA model. Osmotic coefficient values for promethium nitrate and promethium chloride given by this theory are proposed. Finally, water activity measurements were made to examine the fact that the ternary system Ce(NO3)3/HNO3/H2O and the quaternary system Ce(NO3)3/HNO3/N2H5NO3/H2O may be regarded as "simple solutions" (in the sense of Zdanovskii and Mikulin).

  13. Reactions of the class II peroxidases, lignin peroxidase and Arthromyces ramosus peroxidase, with hydrogen peroxide. Catalase-like activity, compound III formation, and enzyme inactivation.

    PubMed

    Hiner, Alexander N P; Hernández-Ruiz, Josefa; Rodríguez-López, José Neptuno; García-Cánovas, Francisco; Brisset, Nigel C; Smith, Andrew T; Arnao, Marino B; Acosta, Manuel

    2002-07-26

    The reactions of the fungal enzymes Arthromyces ramosus peroxidase (ARP) and Phanerochaete chrysosporium lignin peroxidase (LiP) with hydrogen peroxide (H(2)O(2)) have been studied. Both enzymes exhibited catalase activity with hyperbolic H(2)O(2) concentration dependence (K(m) approximately 8-10 mm, k(cat) approximately 1-3 s(-1)). The catalase and peroxidase activities of LiP were inhibited within 10 min and those of ARP in 1 h. The inactivation constants were calculated using two independent methods; LiP, k(i) approximately 19 x 10(-3) s(-1); ARP, k(i) approximately 1.6 x 10(-3) s(-1). Compound III (oxyperoxidase) was detected as the majority species after the addition of H(2)O(2) to LiP or ARP, and its formation was accompanied by loss of enzyme activity. A reaction scheme is presented which rationalizes the turnover and inactivation of LiP and ARP with H(2)O(2). A similar model is applicable to horseradish peroxidase. The scheme links catalase and compound III forming catalytic pathways and inactivation at the level of the [compound I.H(2)O(2)] complex. Inactivation does not occur from compound III. All peroxidases studied to date are sensitive to inactivation by H(2)O(2), and it is suggested that the model will be generally applicable to peroxidases of the plant, fungal, and prokaryotic superfamily.

  14. The SPARX Project: R & D Activity Towards X-Rays FEL Sources

    SciTech Connect

    Alesini, D.; Bellaveglia, M.; Bertolucci, S.; Biagini, M.E.; Boni, R.; Boscolo, M.; Castellano, M.; Clozza, A.; Di Pirro, G.; Drago, A.; Esposito, A.; Ferrario, M.; Filippetto, D.; Fusco, V.; Gallo, A.; Ghigo, A.; Guiducci, S.; Incurvati, M.; Ligi, C.; Marcellini, F.; Migliorati, M.; /Frascati /ENEA, Frascati /INFN, Milan /INFN, Rome /INFN, Rome2 /Milan Polytechnic /UCLA /SLAC

    2005-08-05

    SPARX is an evolutionary project proposed by a collaboration among ENEA-INFN-CNR-Universita di Roma Tor Vergata aiming at the construction of a FELSASE X-ray source in the Tor Vergata Campus. The first phase of the SPARX project, funded by Government Agencies, will be focused on R&D activity on critical components and techniques for future X-ray facilities as described in this paper.

  15. Annual DOE active solar heating and cooling contractors' review meeting. Premeeting proceedings and project summaries

    SciTech Connect

    None,

    1981-09-01

    Ninety-three project summaries are presented which discuss the following aspects of active solar heating and cooling: Rankine solar cooling systems; absorption solar cooling systems; desiccant solar cooling systems; solar heat pump systems; solar hot water systems; special projects (such as the National Solar Data Network, hybrid solar thermal/photovoltaic applications, and heat transfer and water migration in soils); administrative/management support; and solar collector, storage, controls, analysis, and materials technology. (LEW)

  16. Global Positioning System III (GPS III)

    DTIC Science & Technology

    2013-12-01

    Global Positioning System III ( GPS III) As of FY 2015 President’s Budget...00-00-2013 to 00-00-2013 4. TITLE AND SUBTITLE Global Positioning System III ( GPS III) 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT...Responsible Office References Program Name Global Positioning System III ( GPS III) DoD Component Air Force

  17. [Investigation of biologically active compounds at the Department of Organic Chemistry of University of Debrecen 1992-2009. Part III].

    PubMed

    Antus, Sándor

    2010-01-01

    The author briefly reviews the beginning of the carbohydrate chemistry in Hungary with special regard to the results achieved at the Department of Organic Chemistry of University of Debrecen and summarizes the most important synthetic and pharmaceutical results obtained in this field between 1992-2009, part III.

  18. Structure-activity relationships for analogues of the phenazine-based dual topoisomerase I/II inhibitor XR11576.

    PubMed

    Wang, Shouming; Miller, Warren; Milton, John; Vicker, Nigel; Stewart, Alistair; Charlton, Peter; Mistry, Prakash; Hardick, David; Denny, William A

    2002-02-11

    As part of a programme to identify further analogues of the dual topo I/II inhibitor XR11576, we describe here the syntheses and SAR studies of various 'minimal' and 3,4-benzofused phenazine chromophores of the phenazine template of XR11576.

  19. Binding analysis of ytterbium(III) complex containing 1,10-phenanthroline with DNA and its antimicrobial activity.

    PubMed

    Moodi, Asieh; Khorasani-Motlagh, Mozhgan; Noroozifar, Meissam; Niroomand, Sona

    2013-01-01

    To evaluate the biological preference of [Yb(phen)₂(OH₂)Cl₃](H₂O)₂ (phen is 1,10-phenanthroline) for DNA, interaction of Yb(III) complex with DNA in Tris-HCl buffer is studied by various biophysical and spectroscopic techniques which reveal that the complex binds to DNA. The results of fluorescence titration reveal that [Yb(phen)₂(OH₂)Cl₃](H₂O)₂ has strongly quenched in the presence of DNA. The binding site number n, apparent binding constant K b, and the Stern-Volmer quenching constant K SV are determined. ΔH⁰, ΔS⁰, and ΔG⁰ are obtained based on the quenching constants and thermodynamic theory (ΔH⁰ > 0, ΔS⁰ > 0, and ΔG⁰ < 0). The experimental results show that the Yb(III) complex binds to DNA by non-intercalative mode. Groove binding is the preferred mode of interaction for [Yb(phen)₂(OH₂)Cl₃](H₂O)₂ to DNA. The DNA cleavage results show that in the absence of any reducing agent, Yb(III) complex can cleave DNA. The antimicrobial screening tests are also recorded and give good results in the presence of Yb(III) complex.

  20. ISS Internal Active Thermal Control System (IATCS) Coolant Remediation Project

    NASA Technical Reports Server (NTRS)

    Morrison, Russell H.; Holt, Mike

    2005-01-01

    The IATCS coolant has experienced a number of anomalies in the time since the US Lab was first activated on Flight 5A in February 2001. These have included: 1) a decrease in coolant pH, 2) increases in inorganic carbon, 3) a reduction in phosphate buffer concentration, 4) an increase in dissolved nickel and precipitation of nickel salts, and 5) increases in microbial concentration. These anomalies represent some risk to the system, have been implicated in some hardware failures and are suspect in others. The ISS program has conducted extensive investigations of the causes and effects of these anomalies and has developed a comprehensive program to remediate the coolant chemistry of the on-orbit system as well as provide a robust and compatible coolant solution for the hardware yet to be delivered. The remediation steps include changes in the coolant chemistry specification, development of a suite of new antimicrobial additives, and development of devices for the removal of nickel and phosphate ions from the coolant. This paper presents an overview of the anomalies, their known and suspected system effects, their causes, and the actions being taken to remediate the coolant.

  1. Advanced experimental analysis of controls on microbial Fe(III) oxide reduction. First year progress report

    SciTech Connect

    Roden, E.E.; Urrutia, M.M.

    1997-07-01

    'The authors have made considerable progress toward a number of project objectives during the first several months of activity on the project. An exhaustive analysis was made of the growth rate and biomass yield (both derived from measurements of cell protein production) of two representative strains of Fe(III)-reducing bacteria (Shewanellaalga strain BrY and Geobactermetallireducens) growing with different forms of Fe(III) as an electron acceptor. These two fundamentally different types of Fe(III)-reducing bacteria (FeRB) showed comparable rates of Fe(III) reduction, cell growth, and biomass yield during reduction of soluble Fe(III)-citrate and solid-phase amorphous hydrous ferric oxide (HFO). Intrinsic growth rates of the two FeRB were strongly influenced by whether a soluble or a solid-phase source of Fe(III) was provided: growth rates on soluble Fe(III) were 10--20 times higher than those on solid-phase Fe(III) oxide. Intrinsic FeRB growth rates were comparable during reduction of HF0 and a synthetic crystalline Fe(III) oxide (goethite). A distinct lag phase for protein production was observed during the first several days of incubation in solid-phase Fe(III) oxide medium, even though Fe(III) reduction proceeded without any lag. No such lag between protein production and Fe(III) reduction was observed during growth with soluble Fe(III). This result suggested that protein synthesis coupled to solid-phase Fe(III) oxide reduction in batch culture requires an initial investment of energy (generated by Fe(III) reduction), which is probably needed for synthesis of materials (e.g. extracellular polysaccharides) required for attachment of the cells to oxide surfaces. This phenomenon may have important implications for modeling the growth of FeRB in subsurface sedimentary environments, where attachment and continued adhesion to solid-phase materials will be required for maintenance of Fe(III) reduction activity. Despite considerable differences in the rate and pattern

  2. Synthesis, physicochemical characterization, DFT calculation and biological activities of Fe(III) and Co(II)-omeprazole complexes. Potential application in the Helicobacter pylori eradication

    NASA Astrophysics Data System (ADS)

    Russo, Marcos G.; Vega Hissi, Esteban G.; Rizzi, Alberto C.; Brondino, Carlos D.; Salinas Ibañez, Ángel G.; Vega, Alba E.; Silva, Humberto J.; Mercader, Roberto; Narda, Griselda E.

    2014-03-01

    The reaction between the antiulcer agent omeprazole (OMZ) with Fe(III) and Co(II) ions was studied, observing a high ability to form metal complexes. The isolated microcrystalline solid complexes were characterized by elemental analysis, X-ray powder diffraction (XRPD), Scanning Electron Microscopy (SEM), magnetic measurements, thermal study, FTIR, UV-Visible, Mössbauer, electronic paramagnetic resonance (EPR), and DFT calculations. The metal-ligand ratio for both complexes was 1:2 determined by elemental and thermal analysis. FTIR spectroscopy showed that OMZ acts as a neutral bidentate ligand through the pyridinic nitrogen of the benzimidazole ring and the oxygen atom of the sulfoxide group, forming a five-membered ring chelate. Electronic, Mössbauer, and EPR spectra together with magnetic measurements indicate a distorted octahedral geometry around the metal ions, where the coordination sphere is completed by two water molecules. SEM and XRPD were used to characterize the morphology and the crystal nature of the complexes. The most favorable conformation for the Fe(III)-OMZ and Co(II)-OMZ complexes was obtained by DFT calculations by using B3LYP/6-31G(d)&LanL2DZ//B3LYP/3-21G(d)&LanL2DZ basis set. Studies of solubility along with the antibacterial activity against Helicobacter pylori for OMZ and its Co(II) and Fe(III) complexes are also reported. Free OMZ and both metal complexes showed antibacterial activity against H. pylori. Co(II)-OMZ presented a minimal inhibitory concentration ˜32 times lower than that of OMZ and ˜65 lower than Fe(III)-OMZ, revealing its promising potential use for the treatment of gastric pathologies associated with the Gram negative bacteria. The morphological changes observed in the cell membrane of the bacteria after the incubation with the metal-complexes were also analyzed by SEM microscopy. The antimicrobial activity of the complexes was proved by the viability test.

  3. Synergistic Effect of Dual Electron-Cocatalysts for Enhanced Photocatalytic Activity: rGO as Electron-Transfer Mediator and Fe(III) as Oxygen-Reduction Active Site

    PubMed Central

    Yu, Huogen; Tian, Jing; Chen, Feng; Wang, Ping; Wang, Xuefei

    2015-01-01

    For a high-performance cocatalyst-modified photocatalyst, an effective interfacial separation of photogenerated electron from its corresponding holes and its following reduction reaction at the active sites are highly required. However, it is difficult for a single-component cocatalyst to simultaneously realize the crucial functions. In this study, an effective interfacial transfer of photogenerated electrons and its following rapid oxygen-reduction can be easily realized in a dual electron-cocatalyst modified Fe(III)/rGO-TiO2 photocatalyst, where the rGO nanosheets function as an electron-transfer mediator for the effective transfer of photogenerated electrons from the TiO2 surface while the Fe(III) cocatalyst serves as an electron-reduction active site to promote the following interfacial oxygen reduction. In this case, the rGO nanosheets were firstly loaded on the TiO2 nanoparticle surface by a hydrothermal method and then the Fe(III) cocatalyst was further modified on the rGO nanosheets by an impregnation method to prepare the Fe(III)/rGO-TiO2 photocatalyst. It was found that the dual electron-cocatalyst modified Fe(III)/rGO-TiO2 photocatalyst showed an obviously higher photocatalytic performance than the naked TiO2 and single-cocatalyst modified photocatalysts (such as Fe(III)/TiO2 and rGO-TiO2) owing to the synergistic effect of rGO and Fe(III) bi-cocatalysts. The present work can provide some new insights for the smart design of high-efficiency photocatalytic materials. PMID:26272870

  4. Antibacterial activity of peptides derived from the C-terminal region of a hemolytic lectin, CEL-III, from the marine invertebrate Cucumaria echinata.

    PubMed

    Hatakeyama, Tomomitsu; Suenaga, Tomoko; Eto, Seiichiro; Niidome, Takuro; Aoyagi, Haruhiko

    2004-01-01

    Several synthetic peptides derived from the C-terminal domain sequence of a hemolytic lectin, CEL-III, were examined as to their action on bacteria and artificial lipid membranes. Peptide P332 (KGVIFAKASVSVKVTASLSK-NH(2)), corresponding to the sequence from residue 332, exhibited strong antibacterial activity toward Gram-positive bacteria. Replacement of each Lys in P332 by Ala markedly decreased the activity. However, when all Lys were replaced by Arg, the antibacterial activity increased, indicating the importance of positively charged residues at these positions. Replacement of Val by Leu also led to higher antibacterial activity, especially toward Gram-negative bacteria. The antibacterial activity of these peptides was correlated with their membrane-permeabilizing activity toward the bacterial inner membrane and artificial lipid vesicles, indicating that the antibacterial action is due to perturbation of bacterial cell membranes, leading to enhancement of their permeability. These results also suggest that the hydrophobic region of CEL-III, from which P332 and its analogs were derived, may play some role in the interaction with target cell membranes to trigger hemolysis.

  5. Welding III.

    ERIC Educational Resources Information Center

    Allegheny County Community Coll., Pittsburgh, PA.

    Instructional objectives and performance requirements are outlined in this course guide for Welding III, an advanced course in arc welding offered at the Community College of Allegheny County to provide students with the proficiency necessary for industrial certification. The course objectives, which are outlined first, specify that students will…

  6. Synthesis of Isocoumarins from Cyclic 2-Diazo-1,3-diketones and Benzoic Acids via Rh(III)-Catalyzed C-H Activation and Esterification.

    PubMed

    Yang, Cheng; He, Xinwei; Zhang, Lanlan; Han, Guang; Zuo, Youpeng; Shang, Yongjia

    2017-02-17

    A mild and efficient Rh(III)-catalyzed C-H activation/esterification reaction for the synthesis of isocoumarins has been developed. This procedure uses readily available benzoic acids and cyclic diazo-1,3-diketones as starting materials and involves domino intermolecular C-H activation in combination with intramolecular esterification to give the corresponding isocoumarins in moderate to excellent yields. This process provides a facile approach for the construction of isocoumarins containing various functional groups that does not require any additives.

  7. Mechanistic insights on iodine(III) promoted metal-free dual C-H activation involved in the formation of a spirocyclic bis-oxindole.

    PubMed

    Sreenithya, A; Sunoj, Raghavan B

    2014-12-05

    The mechanism of a metal-free, phenyliodine(III) bis(trifluoroacetate) promoted, dual aryl C-H activation of an anilide to a spirocyclic bis-oxindole is examined using density functional theory (M06-2X). The most preferred pathway proceeds through the involvement of a novel iodonium ion intermediate and a pivotal trifluoroacetate counterion. The two sequential aryl C-H activations, assisted by trifluoroacetate as well as the superior leaving group ability of PhI, facilitate the formation of spirocyclic bis-oxindole.

  8. Comparison of the Antiproliferative Activity of Two Antitumour Ruthenium(III) Complexes With Their Apotransferrin and Transferrin-Bound Forms in a Human Colon Cancer Cell Line

    PubMed Central

    Keppler, B. K.; Hartmann, M.; Messori, L.; Berger, M. R.

    1996-01-01

    Two ruthenium(III) complexes, namely trans-indazolium[tetrachlorobis(indazole)- ruthenate(III)], HInd[RuInd2Cl4] and trans-imidazolium[tetrachlorobis(imidazole)- ruthenate(III)], HIm[RuIm2Cl4] exhibit high anticancer activity in an autochthonous colorectal carcinoma model in rats. Recently, it has been shown that both complexes bind specifically to human serum apotransferrin and the resulting adducts have been studied through spectroscopic and chromatographic techniques with the ultimate goal of preparing adducts with good selectivity for cancer cells due to the fact that tumour cells express high amounts of transferrin receptors on their cell surface. In order to investigate whether the cellular uptake of the complexes was mediated by apotransferrin or transferrin, we compared the antiproliferative efficacy of HInd[RuInd2Cl4] and HIm[RuIm2Cl4] with its apotransferrin- and transferrin-bound form in the human colon cancer cell line SW707 using the microculture tetrazolium test (MTT). Our results show that especially the transferrin-bound forms exhibit high antiproliferative activity, which exceeds that of the free complex, indicating that this protein can act as a carrier of the ruthenium complexes into the tumor cell. PMID:18472789

  9. YopN and TyeA Hydrophobic Contacts Required for Regulating Ysc-Yop Type III Secretion Activity by Yersinia pseudotuberculosis

    PubMed Central

    Amer, Ayad A. A.; Gurung, Jyoti M.; Costa, Tiago R. D.; Ruuth, Kristina; Zavialov, Anton V.; Forsberg, Åke; Francis, Matthew S.

    2016-01-01

    Yersinia bacteria target Yop effector toxins to the interior of host immune cells by the Ysc-Yop type III secretion system. A YopN-TyeA heterodimer is central to controlling Ysc-Yop targeting activity. A + 1 frameshift event in the 3-prime end of yopN can also produce a singular secreted YopN-TyeA polypeptide that retains some regulatory function even though the C-terminal coding sequence of this YopN differs greatly from wild type. Thus, this YopN C-terminal segment was analyzed for its role in type III secretion control. Bacteria producing YopN truncated after residue 278, or with altered sequence between residues 279 and 287, had lost type III secretion control and function. In contrast, YopN variants with manipulated sequence beyond residue 287 maintained full control and function. Scrutiny of the YopN-TyeA complex structure revealed that residue W279 functioned as a likely hydrophobic contact site with TyeA. Indeed, a YopNW279G mutant lost all ability to bind TyeA. The TyeA residue F8 was also critical for reciprocal YopN binding. Thus, we conclude that specific hydrophobic contacts between opposing YopN and TyeA termini establishes a complex needed for regulating Ysc-Yop activity. PMID:27446813

  10. Structural, EPR, and Mössbauer characterization of (μ-alkoxo)(μ-carboxylato)diiron(II,III) model complexes for the active sites of mixed-valent diiron enzymes.

    PubMed

    Li, Feifei; Chakrabarti, Mrinmoy; Dong, Yanhong; Kauffmann, Karl; Bominaar, Emile L; Münck, Eckard; Que, Lawrence

    2012-03-05

    To obtain structural and spectroscopic models for the diiron(II,III) centers in the active sites of diiron enzymes, the (μ-alkoxo)(μ-carboxylato)diiron(II,III) complexes [Fe(II)Fe(III)(N-Et-HPTB)(O(2)CPh)(NCCH(3))(2)](ClO(4))(3) (1) and [Fe(II)Fe(III)(N-Et-HPTB)(O(2)CPh)(Cl)(HOCH(3))](ClO(4))(2) (2) (N-Et-HPTB = N,N,N',N'-tetrakis(2-(1-ethyl-benzimidazolylmethyl))-2-hydroxy-1,3-diaminopropane) have been prepared and characterized by X-ray crystallography, UV-visible absorption, EPR, and Mössbauer spectroscopies. Fe1-Fe2 separations are 3.60 and 3.63 Å, and Fe1-O1-Fe2 bond angles are 128.0° and 129.4° for 1 and 2, respectively. Mössbauer and EPR studies of 1 show that the Fe(III) (S(A) = 5/2) and Fe(II) (S(B) = 2) sites are antiferromagnetically coupled to yield a ground state with S = 1/2 (g= 1.75, 1.88, 1.96); Mössbauer analysis of solid 1 yields J = 22.5 ± 2 cm(-1) for the exchange coupling constant (H = JS(A)·S(B) convention). In addition to the S = 1/2 ground-state spectrum of 1, the EPR signal for the S = 3/2 excited state of the spin ladder can also be observed, the first time such a signal has been detected for an antiferromagnetically coupled diiron(II,III) complex. The anisotropy of the (57)Fe magnetic hyperfine interactions at the Fe(III) site is larger than normally observed in mononuclear complexes and arises from admixing S > 1/2 excited states into the S = 1/2 ground state by zero-field splittings at the two Fe sites. Analysis of the "D/J" mixing has allowed us to extract the zero-field splitting parameters, local g values, and magnetic hyperfine structural parameters for the individual Fe sites. The methodology developed and followed in this analysis is presented in detail. The spin Hamiltonian parameters of 1 are related to the molecular structure with the help of DFT calculations. Contrary to what was assumed in previous studies, our analysis demonstrates that the deviations of the g values from the free electron value (g = 2) for the

  11. Evidence That the [beta] Subunit of Chlamydia trachomatis Ribonucleotide Reductase Is Active with the Manganese Ion of Its Manganese(IV)/Iron(III) Cofactor in Site 1

    SciTech Connect

    Dassama, Laura M.K.; Boal, Amie K.; Krebs, Carsten; Rosenzweig, Amy C.; Bollinger, Jr., J. Martin

    2014-10-02

    The reaction of a class I ribonucleotide reductase (RNR) begins when a cofactor in the {beta} subunit oxidizes a cysteine residue {approx}35 {angstrom} away in the {alpha} subunit, generating a thiyl radical. In the class Ic enzyme from Chlamydia trachomatis (Ct), the cysteine oxidant is the Mn{sup IV} ion of a Mn{sup IV}/Fe{sup III} cluster, which assembles in a reaction between O{sub 2} and the Mn{sup II}/Fe{sup II} complex of {beta}. The heterodinuclear nature of the cofactor raises the question of which site, 1 or 2, contains the Mn{sup IV} ion. Because site 1 is closer to the conserved location of the cysteine-oxidizing tyrosyl radical of class Ia and Ib RNRs, we suggested that the Mn{sup IV} ion most likely resides in this site (i.e., {sup 1}Mn{sup IV}/{sup 2}Fe{sup III}), but a subsequent computational study favored its occupation of site 2 ({sup 1}Fe{sup III}/{sup 2}Mn{sup IV}). In this work, we have sought to resolve the location of the Mn{sup IV} ion in Ct RNR-{beta} by correlating X-ray crystallographic anomalous scattering intensities with catalytic activity for samples of the protein reconstituted in vitro by two different procedures. In samples containing primarily Mn{sup IV}/Fe{sup III} clusters, Mn preferentially occupies site 1, but some anomalous scattering from site 2 is observed, implying that both {sup 1}Mn{sup II}/{sup 2}Fe{sup II} and {sup 1}Fe{sup II}/{sup 2}Mn{sup II} complexes are competent to react with O{sub 2} to produce the corresponding oxidized states. However, with diminished Mn{sup II} loading in the reconstitution, there is no evidence for Mn occupancy of site 2, and the greater activity of these 'low-Mn' samples on a per-Mn basis implies that the {sup 1}Mn{sup IV}/{sup 2}Fe{sup III}-{beta} is at least the more active of the two oxidized forms and may be the only active form.

  12. Ligand Design for Rh(III)-Catalyzed C–H Activation: An Unsymmetrical Cyclopentadienyl Enables a Regioselective Synthesis of Dihydroisoquinolones

    PubMed Central

    Hyster, Todd K.; Dalton, Derek M.

    2014-01-01

    We report the regioselective synthesis of dihydroisoquinolones from aliphatic alkenes and O-pivaloyl benzhydroxamic acids mediated by a Rh(III) precatalyst bearing sterically bulky substituents. While the prototypical Cp* ligand provides product with low selectivity, sterically bulky Cpt affords product with excellent regioselectivity for a range of benzhydroxamic acids and alkenes. Crystallographic evidence offers insight as to the source of the increased regioselectivity. PMID:25489470

  13. Cobalt(III) complexes as potential anticancer agents: Physicochemical, structural, cytotoxic activity and DNA/protein interactions.

    PubMed

    Thamilarasan, V; Sengottuvelan, N; Sudha, A; Srinivasan, P; Chakkaravarthi, G

    2016-09-01

    Cobalt(III) complexes (1-3) such as [Co(acac)(bpy)(N3)2·H2O] 1, [Co(acac)(en)(N3)2] 2, and [Co(acac)(2-pic)(N3)2] 3 (where, acac=acetylacetone, bpy=2.2'-bipyridine, en=ethylenediamine, 2-pic=2-picolylamine and NaN3=sodium azide) were synthesized and characterized. The structure of complexes (1-3) has been determined by single crystal X-ray diffraction studies and the configuration around cobalt(III) ion was distorted octahedral coordination geometry. Density functional theory calculations were performed to examine the molecular geometry and frontier molecular orbital properties of complexes (1-3). DNA binding properties of the cobalt(III) complexes with calf thymus DNA (CT-DNA) were investigated by UV-visible absorption, fluorescence, circular dichroism spectroscopy and viscosity measurements. The docking studies showed the preferred orientation of sterically acceptable Co(III) complexes (1, 2) inside the DNA through the mode of intercalation, whereas complex 3 exhibited minor groove binding modes. The intrinsic binding constants Kb of complexes (1-3) with CT-DNA were in the following order 1>3>2. Complexes (1-3) exhibit a good binding propensity to bovine serum albumin (BSA) and gel electrophoresis assay demonstrated that the complexes (1-3) promote the cleavage of the pBR322 DNA in the presence of 3-mercaptopropionic acid (MPA) and cleavage process was found to proceed by singlet oxygen cleavage mechanism. Further, the in vitro cytotoxicity studies of complexes (1-3) were tested on human breast cancer cell line (MCF-7).

  14. Expedient Access to 2,3-Dihydropyridines from Unsaturated Oximes by Rh(III)-Catalyzed C-H Activation.

    PubMed

    Romanov-Michailidis, Fedor; Sedillo, Kassandra F; Neely, Jamie M; Rovis, Tomislav

    2015-07-22

    α,β-Unsaturated oxime pivalates are proposed to undergo reversible C(sp(2))-H insertion with cationic Rh(III) complexes to furnish five-membered metallacycles. In the presence of 1,1-disubstituted olefins, these species participate in irreversible migratory insertion to give, after reductive elimination, 2,3-dihydropyridine products in good yields. Catalytic hydrogenation can then be used to convert these molecules into piperidines, which are important structural components of numerous pharmaceuticals.

  15. Abundance, Distribution, and Activity of Fe(II)-Oxidizing and Fe(III)-Reducing Microorganisms in Hypersaline Sediments of Lake Kasin, Southern Russia

    PubMed Central

    Emmerich, Maren; Bhansali, Ankita; Lösekann-Behrens, Tina; Schröder, Christian; Kappler, Andreas

    2012-01-01

    The extreme osmotic conditions prevailing in hypersaline environments result in decreasing metabolic diversity with increasing salinity. Various microbial metabolisms have been shown to occur even at high salinity, including photosynthesis as well as sulfate and nitrate reduction. However, information about anaerobic microbial iron metabolism in hypersaline environments is scarce. We studied the phylogenetic diversity, distribution, and metabolic activity of iron(II)-oxidizing and iron(III)-reducing Bacteria and Archaea in pH-neutral, iron-rich salt lake sediments (Lake Kasin, southern Russia; salinity, 348.6 g liter−1) using a combination of culture-dependent and -independent techniques. 16S rRNA gene clone libraries for Bacteria and Archaea revealed a microbial community composition typical for hypersaline sediments. Most-probable-number counts confirmed the presence of 4.26 × 102 to 8.32 × 103 iron(II)-oxidizing Bacteria and 4.16 × 102 to 2.13 × 103 iron(III)-reducing microorganisms per gram dry sediment. Microbial iron(III) reduction was detected in the presence of 5 M NaCl, extending the natural habitat boundaries for this important microbial process. Quantitative real-time PCR showed that 16S rRNA gene copy numbers of total Bacteria, total Archaea, and species dominating the iron(III)-reducing enrichment cultures (relatives of Halobaculum gomorrense, Desulfosporosinus lacus, and members of the Bacilli) were highest in an iron oxide-rich sediment layer. Combined with the presented geochemical and mineralogical data, our findings suggest the presence of an active microbial iron cycle at salt concentrations close to the solubility limit of NaCl. PMID:22504804

  16. Tungsten disulfide nanosheet and exonuclease III co-assisted amplification strategy for highly sensitive fluorescence polarization detection of DNA glycosylase activity.

    PubMed

    Zhao, Jingjin; Ma, Yefei; Kong, Rongmei; Zhang, Liangliang; Yang, Wen; Zhao, Shulin

    2015-08-05

    Herein, we introduced a tungsten disulfide (WS2) nanosheet and exonuclease III (Exo III) co-assisted signal amplification strategy for highly sensitive fluorescent polarization (FP) assay of DNA glycosylase activity. Two DNA glycosylases, uracil-DNA glycosylase (UDG) and human 8-oxoG DNA glycosylase 1 (hOGG1), were tested. A hairpin-structured probe (HP) which contained damaged bases in the stem was used as the substrate. The removal of damaged bases from substrate by DNA glycosylase would lower the melting temperature of HP. The HP was then opened and hybridized with a FAM dye-labeled single strand DNA (DP), generating a duplex with a recessed 3'-terminal of DP. This design facilitated the Exo III-assisted amplification by repeating the hybridization and digestion of DP, liberating numerous FAM fluorophores which could not be adsorbed on WS2 nanosheet. Thus, the final system exhibited a small FP signal. However, in the absence of DNA glycosylases, no hybridization between DP and HP was occurred, hampering the hydrolysis of DP by Exo III. The intact DP was then adsorbed on the surface of WS2 nanosheet that greatly amplified the mass of the labeled-FAM fluorophore, resulting in a large FP value. With the co-assisted amplification strategy, the sensitivity was substantially improved. In addition, this method was applied to detect UDG activity in cell extracts. The study of the inhibition of UDG was also performed. Furthermore, this method is simple in design, easy in implementation, and selective, which holds potential applications in the DNA glycosylase related mechanism research and molecular diagnostics.

  17. New bipyridine gold(III) dithiocarbamate-containing complexes exerted a potent anticancer activity against cisplatin-resistant cancer cells independent of p53 status

    PubMed Central

    Altaf, Muhammad; Monim-ul-Mehboob, Muhammad; Kawde, Abdel-Nasser; Corona, Giuseppe; Larcher, Roberto; Ogasawara, Marcia; Casagrande, Naike; Celegato, Marta; Borghese, Cinzia; Siddik, Zahid H.; Aldinucci, Donatella; Isab, Anvarhusein A.

    2017-01-01

    We synthesized, characterized and tested in a panel of cancer cell lines, nine new bipyridine gold(III) dithiocarbamate-containing complexes. In vitro studies demonstrated that compounds 1, 2, 4, 5, 7 and 8 were the most cytotoxic in prostate, breast, ovarian cancer cell lines and in Hodgkin lymphoma cells with IC50 values lower than the reference drug cisplatin. The most active compound 1 was more active than cisplatin in ovarian (A2780cis and 2780CP-16) and breast cancer cisplatin-resistant cells. Compound 1 determined an alteration of the cellular redox homeostasis leading to increased ROS levels, a decrease in the mitochondrial membrane potential, cytochrome-c release from the mitochondria and activation of caspases 9 and 3. The ROS scavenger NAC suppressed ROS generation and rescued cells from damage. Compound 1 resulted more active in tumor cells than in normal human Mesenchymal stromal cells. Gold compounds were active independent of p53 status: exerted cytotoxic effects on a panel of non-small cell lung cancer cell lines with different p53 status and in the ovarian A2780 model where the p53 was knocked out. In conclusion, these promising results strongly indicate the need for further preclinical evaluation to test the clinical potential of these new gold(III) complexes. PMID:27888799

  18. Challenges in mapping behaviours to activities using logs from a citizen science project

    NASA Astrophysics Data System (ADS)

    Morais, Alessandra M. M.; Guarino de Vasconcelos, Leandro; Santos, Rafael D. C.

    2016-05-01

    Citizen science projects are those which recruit volunteers to participate as assistants in scientific studies. Since these projects depend on volunteer efforts, understanding the motivation that drives a volunteer to collaborate is important to ensure its success. One way to understand motivation is by interviewing the volunteers. While this approach may elicit detailed information on the volunteers' motivation and actions, it is restricted to a subset of willing participants. For web-based projects we could instead use logs of volunteers' activities, which measures which volunteer did what and when for all volunteers in a project. In this work we present some metrics that can be calculated from the logs, based on a model of interaction. We also comment on the applicability of those metrics, describe an ongoing work that may yield more precise logs and metrics and comment on issues for further research.

  19. Integrating Field-Centered, Project Based Activities with Academic Year Coursework: A Curriculum Wide Approach

    NASA Astrophysics Data System (ADS)

    Kelso, P. R.; Brown, L. M.

    2015-12-01

    Based upon constructivist principles and the recognition that many students are motivated by hands-on activities and field experiences, we designed a new undergraduate curriculum at Lake Superior State University. One of our major goals was to develop stand-alone field projects in most of the academic year courses. Examples of courses impacted include structural geology, geophysics, and geotectonics, Students learn geophysical concepts in the context of near surface field-based geophysical studies while students in structural geology learn about structural processes through outcrop study of fractures, folds and faults. In geotectonics students learn about collisional and rifting processes through on-site field studies of specific geologic provinces. Another goal was to integrate data and samples collected by students in our sophomore level introductory field course along with stand-alone field projects in our clastic systems and sequence stratigraphy courses. Our emphasis on active learning helps students develop a meaningful geoscience knowledge base and complex reasoning skills in authentic contexts. We simulate the activities of practicing geoscientists by engaging students in all aspects of a project, for example: field-oriented project planning and design; acquiring, analyzing, and interpreting data; incorporating supplemental material and background data; and preparing oral and written project reports. We find through anecdotal evidence including student comments and personal observation that the projects stimulate interest, provide motivation for learning new concepts, integrate skill and concept acquisition vertically through the curriculum, apply concepts from multiple geoscience subdisiplines, and develop soft skills such as team work, problem solving, critical thinking and communication skills. Through this projected-centered Lake Superior State University geology curriculum students practice our motto of "learn geology by doing geology."

  20. ACFA 2020 - An FP7 project on active control of flexible fuel efficient aircraft configurations

    NASA Astrophysics Data System (ADS)

    Maier, R.

    2013-12-01

    This paper gives an overview about the project ACFA 2020 which is funded by the European Commission within the 7th framework program. The acronym ACFA 2020 stands for Active Control for Flexible Aircraft 2020. The project is dealing with the design of highly fuel efficient aircraft configurations and, in particular, on innovative active control concepts with the goal to reduce loads and structural weight. Major focus lays on blended wing body (BWB) aircraft. Blended wing body type aircraft configurations are seen as the most promising future concept to fulfill the so-called ACARE (Advisory Council for Aeronautics Research in Europe) vision 2020 goals in regards to reduce fuel consumption and external noise. The paper discusses in some detail the overall goals and how they are addressed in the workplan. Furthermore, the major achievements of the project are outlined and a short outlook on the remaining work is given.

  1. Design requirements document for Project W-465, immobilized low-activity waste interim storage

    SciTech Connect

    Burbank, D.A.

    1998-05-19

    The scope of this Design Requirements Document (DRD) is to identify the functions and associated requirements that must be performed to accept, transport, handle, and store immobilized low-activity waste (ILAW) produced by the privatized Tank Waste Remediation System (TWRS) treatment contractors. The functional and performance requirements in this document provide the basis for the conceptual design of the TWRS ILAW Interim Storage facility project and provides traceability from the program level requirements to the project design activity. Technical and programmatic risk associated with the TWRS planning basis are discussed in the Tank Waste Remediation System Decisions and Risk Assessment (Johnson 1994). The design requirements provided in this document will be augmented by additional detailed design data documented by the project.

  2. Thermodynamics, Kinetics, and Activation energy Studies of the sorption of chromium(III) and chromium(VI) to a Mn3O4 nanomaterial

    PubMed Central

    Cantu, Yvette; Remes, Abril; Reyna, Alejandra; Martinez, Denise; Villarreal, Jahaziel; Ramos, Hilda; Trevino, Samantha; Tamez, C.; Martinez, A.; Eubanks, T.; Parsons, J. G.

    2014-01-01

    In this study, a manganese oxide, Mn3O4 was used to remove chromium(III) and chromium(VI) from aqueous solutions. The Mn3O4 nanomaterial was synthesized through a precipitation method, and was characterized using XRD, which confirmed the material had a crystal structure similar to hausmannite. In addition, using Scherrer’s equation it was determined that the nanomaterial had an average grain size of 19.5 ± 1.10 nm. A study of the effects of pH on the binding of chromium(III) and chromium(VI) showed that the optimum binding pH was 4 and 3 respectively. Batch isotherm studies were performed to determine the binding capacity of chromium(III), which was determined to be 18.7 mg/g, 41.7 mg/g, and 54.4 mg/g respectively for 4°C, 21°C, and 45°C. Chromium(VI) on the other hand had lower binding capacities of 2.5 mg/g, 4.3 mg/g, and 5.8 mg/g for 4°C, 21°C, 45°C, respectively. Thermodynamic studies performed indicated the sorption process was for the most part controlled by physisorption. The ΔG for the sorption of chromium(III) and Chromium(VI) ranged from −0.9 to −13 kJ/mol, indicating a spontaneous reaction was occurring. The enthalpy indicated a endothermic reaction was occurring during the binding and show ΔH values of 70.6 and 19.1 kJ.mol for chromium(III) and Chromium(VI), respectively. In addition, ΔS for the reaction had positive values of 267 and 73 J/mol for chromium(III) and chromium(VI) which indicate a spontaneous reaction. In addition, the sorption process was found to follow pseudo second order kinetic and the activation energy studies indicated the binding process occurred through chemisorption. PMID:25097453

  3. The RAP: A Recreational Activities Project, Academic Service-Learning Course and Qualitative Research Study

    ERIC Educational Resources Information Center

    Parker, Kathlyn

    2009-01-01

    The author (a university instructor) and her community partner (a public school teacher) have collaborated in teaching an academic service-learning course in special education. This collaboration, the RAP (recreational activities project), was completed by university undergraduate students and young adults with cognitive impairment and/or…

  4. Design and Implementation of a Project-Based Active/Cooperative Engineering Design Course for Freshmen

    ERIC Educational Resources Information Center

    Abdulaal, R. M.; Al-Bahi, A. M.; Soliman, A. Y.; Iskanderani, F. I.

    2011-01-01

    A project-based active/cooperative design course is planned, implemented, assessed and evaluated to achieve several desired engineering outcomes. The course allows freshman-level students to gain professional hands-on engineering design experience through an opportunity to practise teamwork, quality principles, communication skills, life-long…

  5. Video: Animals; Electric Current; Force; Science Activities. Learning in Science Project. Working Papers 51-54.

    ERIC Educational Resources Information Center

    Bell, Beverley; And Others

    Four papers to be used in conjunction with video-tapes developed by the Learning in Science Project are presented. Topic areas of the papers focus on: (1) animals; (2) electric current; (3) force; and (4) science activities. The first paper presents transcripts of class discussions focusing on the scientific meaning of the word animal. The second…

  6. Using Sales Management Students to Manage Professional Selling Students in an Innovative Active Learning Project

    ERIC Educational Resources Information Center

    Young, Joyce A.; Hawes, Jon M.

    2013-01-01

    This paper describes an application of active learning within two different courses: professional selling and sales management. Students assumed the roles of sales representatives and sales managers for an actual fund-raiser--a golf outing--sponsored by a student chapter of the American Marketing Association. The sales project encompassed an…

  7. 34 CFR 647.4 - What activities and services may a project provide?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... programs. (g) Mentoring programs involving faculty members or students at institutions of higher education... 34 Education 3 2010-07-01 2010-07-01 false What activities and services may a project provide? 647.4 Section 647.4 Education Regulations of the Offices of the Department of Education...

  8. Using Interdisciplinary, Project-Based, Multimodal Activities to Facilitate Literacy across the Content Areas

    ERIC Educational Resources Information Center

    Hill, Archie E.

    2014-01-01

    Tour Across America is an interdisciplinary, project-based, multimodal (IPM) activity where students get the opportunity to apply what they learn to a real-life situation while illustrating the interdisciplinary nature of literacy. It provides students with a high-interest, creative platform to review, reinforce, and integrate learned literacy…

  9. Students' Network Project Activities in the Context of the Information Educational Medium of Higher Education Institution

    ERIC Educational Resources Information Center

    Samerkhanova, Elvira K.; Krupoderova, Elena P.; Krupoderova, Klimentina R.; Bahtiyarova, Lyudmila N.; Ponachugin, Alexander V.

    2016-01-01

    The purpose of the research is justifying didactic possibilities of the use of network services for the organization of information for the learning environment of college, where students carry out their project activities, and where effective networking between students and teachers takes place. The authors consider didactic possibilities of…

  10. Secondary English Activities of the North Dakota Exemplary Project in Career Education. Grades 7-12.

    ERIC Educational Resources Information Center

    North Dakota State Board for Vocational Education, Bismarck.

    The secondary English activities materials developed by the North Dakota Exemplary Project represent information that will be helpful to teachers in achieving the goals of a career education program. The guide provides a flexible framework to provide experiences that will support the learning principles needed to attain the required knowledge,…

  11. A Handbook of Projects and Activities for Marketing and Distributive Education.

    ERIC Educational Resources Information Center

    Montana State Office of the Superintendent of Public Instruction, Helena.

    This handbook has been designed to provide classroom teachers with relevant, ready-to-use materials geared to supplement classroom instruction in marketing and distributive education. The projects/activities have been organized using the major units of instruction of the Montana Curriculum Guidelines for Distributive Education, except for a…

  12. Can Activity Projects Improve Children's Wellbeing during the Transition to Secondary Education?

    ERIC Educational Resources Information Center

    Akister, Jane; Guest, Hannah; Burch, Sarah

    2016-01-01

    Promoting child mental wellbeing is an important part of UK early intervention policy. Children with poor physical or mental health have significantly lower educational attainment and lower social status as adults. "Activity" projects are one form of early intervention used to try and help vulnerable children. Evidence relating to the…

  13. Implementation of the Project "Including Disabled Senior Citizens in Creative Activities in 2013-2015"

    ERIC Educational Resources Information Center

    Ploch, Leszek

    2015-01-01

    This paper made an attempt to indicate the findings of the author's research from the experiences of the implementation of the project "Including disabled senior citizens in creative activities in 2013-2015". The issues of disabled senior citizens have been an object of interest over the recent years though it still has not had a proper…

  14. Formative Evaluation of Project "TEAM" (Teens Making Environment and Activity Modifications)

    ERIC Educational Resources Information Center

    Kramer, Jessica M.; Roemer, Kristin; Liljenquist, Kendra; Shin, Julia; Hart, Stacy

    2014-01-01

    Research documents the negative impact of physical and social environmental barriers on engagement in school, work, and the community for youth with intellectual and /or developmental disabilities (IDD). Project "TEAM" (Teens making Environment and Activity Modifications) was designed to teach youth to systematically identify…

  15. Innovative Educational Activities for Disabled and Gifted Students. Selected Projects from the Minigrant Program.

    ERIC Educational Resources Information Center

    Haim, Ytje Politiek

    Described is a selection of the 1981-1985 awards given by the Foundation for Exceptional Children's Bill Geer Minigrant Award Program, which supports the creation of innovative educational activities to benefit handicapped or gifted students from preschool through secondary levels. For each grant, information provided includes: project director,…

  16. 34 CFR 694.21 - What are required activities for GEAR UP projects?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 4 2013-07-01 2013-07-01 false What are required activities for GEAR UP projects? 694.21 Section 694.21 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION (CONTINUED) GAINING EARLY AWARENESS...

  17. 34 CFR 694.21 - What are required activities for GEAR UP projects?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 4 2012-07-01 2012-07-01 false What are required activities for GEAR UP projects? 694.21 Section 694.21 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION (CONTINUED) GAINING EARLY AWARENESS...

  18. Locating Active Plate Boundaries by Earthquake Data. Crustal Evaluation Education Project. Teacher's Guide [and] Student Investigation.

    ERIC Educational Resources Information Center

    Stoever, Edward C., Jr.

    Crustal Evolution Education Project (CEEP) modules were designed to: (1) provide students with the methods and results of continuing investigations into the composition, history, and processes of the earth's crust and the application of this knowledge to man's activities and (2) to be used by teachers with little or no previous background in the…

  19. Effects of Implementing STEM-I Project-Based Learning Activities for Female High School Students

    ERIC Educational Resources Information Center

    Lou, Shi-Jer; Tsai, Huei-Yin; Tseng, Kuo-Hung; Shih, Ru-Chu

    2014-01-01

    This study aims to explore the application of STEM-I (STEM-Imagination) project-based learning activities and its effects on the effectiveness, processes, and characteristics of STEM integrative knowledge learning and imagination development for female high school students. A total of 72 female high school students were divided into 18 teams.…

  20. NIF Title III engineering plan

    SciTech Connect

    Deis, G

    1998-06-01

    The purpose of this document is to define the work that must be accomplished by the NIF Project during Title III Engineering. This definition is intended to be sufficiently detailed to provide a framework for yearly planning, to clearly identify the specific deliverables so that the Project teams can focus on them, and to provide a common set of objectives and processes across the Project. This plan has been preceded by similar documents for Title I and Title II design and complements the Site Management Plan, the Project Control Manual, the Quality Assurance Program Plan, the RM Parsons NIF Title III Configuration Control Plan, the Integrated Project Schedule, the Preliminary Safety Analysis Report, the Configuration Management Plan, and the Transition Plan.