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Sample records for activity pa programs

  1. Accreditation and implications of clinical postgraduate PA training programs.

    PubMed

    Hussaini, Sobia S; Bushardt, Reamer L; Gonsalves, Wanda C; Hilton, Virginia O; Hornberger, Brad J; Labagnara, Frank A; OʼHara, Kevin M; Sasek, Cody; Smith, Benjamin J; Williams, Jennifer S

    2016-05-01

    No consensus definition exists for postgraduate physician assistant (PA) training. This report from the AAPA Task Force on Accreditation of Postgraduate PA Training Programs describes the types of clinical training programs and their effects on hiring and compensation of PAs. Although completing a postgraduate program appears to have no effect on compensation, PAs who complete these programs may be favored in the hiring process and frequently report greater confidence in their skills. More research is needed and program accreditation is key to monitoring the effectiveness of these programs.

  2. Proteasome activators, PA28γ and PA200, play indispensable roles in male fertility.

    PubMed

    Huang, Lin; Haratake, Kousuke; Miyahara, Hatsumi; Chiba, Tomoki

    2016-03-22

    Protein degradation mediated by the proteasome is important for the protein homeostasis. Various proteasome activators, such as PA28 and PA200, regulate the proteasome function. Here we show double knockout (dKO) mice of Psme3 and Psme4 (genes for PA28γ and PA200), but not each single knockout mice, are completely infertile in male. The dKO sperms exhibited remarkable defects in motility, although most of them showed normal appearance in morphology. The proteasome activity of the mutant sperms decreased notably, and the sperms were strongly positive with ubiquitin staining. Quantitative analyses of proteins expressed in dKO sperms revealed up-regulation of several proteins involved in oxidative stress response. Furthermore, increased 8-OHdG staining was observed in dKO sperms head, suggesting defective response to oxidative damage. This report verified PA28γ and PA200 play indispensable roles in male fertility, and provides a novel insight into the role of proteasome activators in antioxidant response.

  3. Proteasome activators, PA28γ and PA200, play indispensable roles in male fertility

    PubMed Central

    Huang, Lin; Haratake, Kousuke; Miyahara, Hatsumi; Chiba, Tomoki

    2016-01-01

    Protein degradation mediated by the proteasome is important for the protein homeostasis. Various proteasome activators, such as PA28 and PA200, regulate the proteasome function. Here we show double knockout (dKO) mice of Psme3 and Psme4 (genes for PA28γ and PA200), but not each single knockout mice, are completely infertile in male. The dKO sperms exhibited remarkable defects in motility, although most of them showed normal appearance in morphology. The proteasome activity of the mutant sperms decreased notably, and the sperms were strongly positive with ubiquitin staining. Quantitative analyses of proteins expressed in dKO sperms revealed up-regulation of several proteins involved in oxidative stress response. Furthermore, increased 8-OHdG staining was observed in dKO sperms head, suggesting defective response to oxidative damage. This report verified PA28γ and PA200 play indispensable roles in male fertility, and provides a novel insight into the role of proteasome activators in antioxidant response. PMID:27003159

  4. Tissue Plasminogen Activator (tPA) Mediates Neurotoxin-Induced Cell Death and Microglial Activation

    DTIC Science & Technology

    2001-07-01

    Alzheimer’s disease and stroke. Tissue plasminogen activator (tPA), a protease converting plasminogen to plasmin, is necessary for neurodegeneration. In mice lacking tPA (tPA-/1), neurons are resistant to neurotoxic death. Delivery of tPA into tpA-/- mice restores susceptibility to neuronal death, indicating that tPA is neurotoxic in the context of excitotoxic injury. Although tPA is synthesized by neurons, the increase in tPA upon injury derives primarily from activated microglia, the immune cells of the brain. Microglia in tPA-/- mice demonstrate reduced activation.

  5. Proteasome Activators, PA28α and PA28β, Govern Development of Microvascular Injury in Diabetic Nephropathy and Retinopathy

    PubMed Central

    Mahmoudpour, Ali

    2016-01-01

    Diabetic nephropathy (DN) and diabetic retinopathy (DR) are major complications of type 1 and type 2 diabetes. DN and DR are mainly caused by injury to the perivascular supporting cells, the mesangial cells within the glomerulus, and the pericytes in the retina. The genes and molecular mechanisms predisposing retinal and glomerular pericytes to diabetic injury are poorly characterized. In this study, the genetic deletion of proteasome activator genes, PA28α and PA28β genes, protected the diabetic mice in the experimental STZ-induced diabetes model against renal injury and retinal microvascular injury and prolonged their survival compared with wild type STZ diabetic mice. The improved wellbeing and reduced renal damage was associated with diminished expression of Osteopontin (OPN) and Monocyte Chemoattractant Protein-1 (MCP-1) in the glomeruli of STZ-injected PA28α/PA28β double knockout (Pa28αβDKO) mice and also in cultured mesangial cells and retinal pericytes isolated from Pa28αβDKO mice that were grown in high glucose. The mesangial PA28-mediated expression of OPN under high glucose conditions was suppressed by peptides capable of inhibiting the binding of PA28 to the 20S proteasome. Collectively, our findings demonstrate that diabetic hyperglycemia promotes PA28-mediated alteration of proteasome activity in vulnerable perivascular cells resulting in microvascular injury and development of DN and DR. PMID:27830089

  6. Model Program: Unionville High School, Kennett Square, PA

    ERIC Educational Resources Information Center

    Berkeihiser, Mike

    2008-01-01

    After attending a conference session about marketing, the author and his colleagues were inspired to start their own marketing program for the technology education program at Unionville High School in Kennett Square, Pennsylvania. When they started, they had no idea how much that simple marketing program would pay off. Over the past seven years,…

  7. Model Program: Southern Lehigh High School, Center Valley, PA

    ERIC Educational Resources Information Center

    Colelli, Richard

    2009-01-01

    In this article, the author describes the technology education program at Southern Lehigh High School, Center Valley, Pennsylvania. The school district is presently providing an educational program known for its excellence and forward-looking perspective, which is sensitive to the changing needs of its students. Within the technology education…

  8. Facing the PA team growth problem in space programs: The Hermes approach

    NASA Astrophysics Data System (ADS)

    Bernede, G.

    1991-08-01

    The increasing need for product assurance (PA) in space programs makes finding skilled and trained engineers willing to work in the PA field a top level difficulty. Few engineers are available and exchanging them from one project to another is not a solution. No reservoir of talent exists for the time being in Europe. Educational efforts have started in several countries to have product assurance techniques become part of basic engineering knowledge. These efforts will bring strong improvement compared to today's situation but not in time for programs such as Hermes. Peculiar solutions must be developed. Approaches used to solve this problem within the Hermes program are outlined.

  9. Crystal structure of an avian influenza polymerase PA[subscript N] reveals an endonuclease active site

    SciTech Connect

    Yuan, Puwei; Bartlam, Mark; Lou, Zhiyong; Chen, Shoudeng; Zhou, Jie; He, Xiaojing; Lv, Zongyang; Ge, Ruowen; Li, Xuemei; Deng, Tao; Fodor, Ervin; Rao, Zihe; Liu, Yingfang

    2009-11-10

    The heterotrimeric influenza virus polymerase, containing the PA, PB1 and PB2 proteins, catalyses viral RNA replication and transcription in the nucleus of infected cells. PB1 holds the polymerase active site and reportedly harbours endonuclease activity, whereas PB2 is responsible for cap binding. The PA amino terminus is understood to be the major functional part of the PA protein and has been implicated in several roles, including endonuclease and protease activities as well as viral RNA/complementary RNA promoter binding. Here we report the 2.2 angstrom (A) crystal structure of the N-terminal 197 residues of PA, termed PA(N), from an avian influenza H5N1 virus. The PA(N) structure has an alpha/beta architecture and reveals a bound magnesium ion coordinated by a motif similar to the (P)DX(N)(D/E)XK motif characteristic of many endonucleases. Structural comparisons and mutagenesis analysis of the motif identified in PA(N) provide further evidence that PA(N) holds an endonuclease active site. Furthermore, functional analysis with in vivo ribonucleoprotein reconstitution and direct in vitro endonuclease assays strongly suggest that PA(N) holds the endonuclease active site and has critical roles in endonuclease activity of the influenza virus polymerase, rather than PB1. The high conservation of this endonuclease active site among influenza strains indicates that PA(N) is an important target for the design of new anti-influenza therapeutics.

  10. A novel plasminogen activator from Agkistrodon blomhoffii Ussurensis venom (ABUSV-PA): purification and characterization.

    PubMed

    Liu, Shuqing; Sun, Ming-Zhong; Greenaway, Frederick T

    2006-10-06

    A plasminogen activator with arginine ester hydrolysis activity (ABUSV-PA) has been identified and purified to homogeneity from Chinese Agkistrodon blomhoffii Ussurensis snake venom. ABUSV-PA, a monomeric protein with molecular mass of 27815.2 Da, was purified 180-fold with 0.02% recovery for protein and 3.6% recovery for esterase activity. ABUSV-PA reacts optimally with its substrate N(alpha)-tosyl-l-arginine-methyl ester (TAME) at approximately pH 7.5 and at 51 degrees C. Measurement from inductively coupled plasma-atomic emission spectroscopy (ICP-AES) reveals that ABUSV-PA is a Zn(2+)-containing protein with a stoichiometry of 1:1 [Zn(2+)]:[ABUSV-PA]. Analyses of esterase hydrolysis and UV absorption and CD spectra indicate that Zn(2+) plays an important role in maintaining the structural integrity rather than the esterase activity of ABUSV-PA. Divalent metal ions, including Ca(2+), Mg(2+), Cu(2+), Ni(2+), Mn(2+), and Co(2+), increase the TAME hydrolysis activity of ABUSV-PA. A red-shift of the emission wavelengths of the synchronous fluorescence of ABUSV-PA, compared to those of free Tyr and Trp, indicates a conformation where the Tyr and Trp residues are in exposed hydrophilic environments. The presence of zinc increases the hydrophobicity of the conformational environments surrounding the Trp residues of ABUSV-PA and affects the secondary structure of ABUSV-PA, as proved by UV absorption and CD spectroscopy.

  11. Genomic structural characterization and transcriptional expression analysis of proteasome activator PA28α and PA28β subunits from Oplegnathus fasciatus.

    PubMed

    Kasthuri, Saranya Revathy; Umasuthan, Navaneethaiyer; Whang, Ilson; Kim, Eunmi; Park, Hae-Chul; Lee, Jehee

    2013-10-01

    Proteasomes are multicatalytic subunit complexes involved in the degradation of cytosolic proteins and antigen presentation. In this study, we have characterized the alpha and beta subunits of proteasome activator complex from rock bream at the molecular level. RbPA28α and RbPA28β possessed the characteristic features of the subunits identified from mammals and teleosts. The RbPA28α and RbPA28β proteasome subunits contained a proline-rich motif (Region A), subunit-specific insert in the region corresponding to the KEKE motif of the known PA28α (Region B), conserved activation loop (Region C), a potential protein kinase C recognition site (Region D) and a highly homologous C-terminal region (Region E) among all three PA28 subunits. Multiple sequence alignment and pairwise alignment revealed that RbPA28α and RbPA28β proteins shared high homology with the teleosts and mammals. RbPA28α and RbPA28β genome possessed 11 exons interrupted by 10 introns. In silico promoter analysis of RbPA28α and RbPA28β revealed various transcription factor-binding sites displaying their regulation under various stress conditions. Tissue distribution profiling showed a higher expression in blood and gills. Transcriptional expression analysis of RbPA28α and RbPA28β showed up-regulation in the immune tissues following LPS and poly I:C challenges, providing further evidence for the immunological role of RbPA28α and RbPA28β.

  12. Physical activity (PA) among middle-aged women: initial and current influences and patterns of participation.

    PubMed

    Codina, Nuria; Pestana, José V; Armadans, Immaculada

    2013-01-01

    This study investigated the initial and current influences for doing physical activity (PA), current levels of PA participation, and future plans for it. Participants were 200 women aged 45 to 64 years old. Factor loadings of influences were explored using Principal Components Analysis. Pearson bivariate correlations, t-test, and ANOVA were used to show the differences among the influences, sociodemographic characteristics, and present/future PA participation. Personal fulfillment was the main initial influence, while health benefits/self-care, and outdoor/family activities were the most important current influences. The results highlight the factors that best explain present PA participation and also plans for activity in the future.

  13. PA-EFL: A Phonological Awareness Program For Indigenous EFL Students with Hearing Disabilities.

    ERIC Educational Resources Information Center

    Yonovitz, L.; Yonovitz, A.; Palmer, Juan C.

    2000-01-01

    Australia's indigenous populations have endemic levels of otitis media with conductive hearing loss. PA-EFL is a phonological awareness program designed for indigenous Australians who are at high risk for hearing disabilities and who are learning English as a foreign language (EFL). Hearing support services were provided; remarkable literacy gains…

  14. Interaction between plasminogen activator inhibitor type 1 (PAI-1) bound to fibrin and either tissue-type plasminogen activator (t-PA) or urokinase-type plasminogen activator (u-PA). Binding of t-PA/PAI-1 complexes to fibrin mediated by both the finger and the kringle-2 domain of t-PA.

    PubMed Central

    Wagner, O F; de Vries, C; Hohmann, C; Veerman, H; Pannekoek, H

    1989-01-01

    Plasminogen activation is catalyzed both by tissue-type-(t-PA) and by urokinase-type plasminogen activator (u-PA). This reaction is controlled by plasminogen activator inhibitor type 1 (PAI-1) that is either present in plasma or bound to fibrin, present in a thrombus. We studied the mechanism of in vitro inhibition of both t-PA and u-PA activity by PAI-1 bound to fibrin. It is shown that activation of latent PAI-1 unmasks a specific fibrin-binding site that is distinct from its reactive site. This reactive site of activated PAI-1 bound to fibrin is fully exposed to form complexes with t-PA and u-PA, that are unable to activate plasminogen. Upon complex formation with either one of the plasminogen activators, PAI-1 apparently undergoes a conformational change and loses its affinity for fibrin. Consequently, complexes of u-PA and PAI-1 dissociate from the fibrin matrix and are encountered in the fluid phase. In contrast, t-PA/PAI-1 complexes remain bound to fibrin. By employing recombinant t-PA deletion-mutant proteins, that precisely lack domains involved in fibrin binding, we demonstrate that binding of t-PA/PAI-1 complexes is mediated by both the "finger" (F) and the "kringle-2" (K2) domain of t-PA. A model is proposed that explains inhibition of the fibrinolytic process, at the level of plasminogen activation by t-PA, directed by PAI-1 bound to fibrin. An implication of the proposed model is that t-PA/PAI-1 complexes and free t-PA compete for the same binding sites on fibrin. Images PMID:2503541

  15. Thrombolytic efficacy and enzymatic activity of rt-PA-loaded echogenic liposomes.

    PubMed

    Bader, Kenneth B; Bouchoux, Guillaume; Peng, Tao; Klegerman, Melvin E; McPherson, David D; Holland, Christy K

    2015-08-01

    Echogenic liposomes (ELIP), that can encapsulate both recombinant tissue-type plasminogen activator (rt-PA) and microbubbles, are under development to improve the treatment of thrombo-occlusive disease. However, the enzymatic activity, thrombolytic efficacy, and stable cavitation activity generated by this agent has yet to be evaluated and compared to another established ultrasound-enhanced thrombolytic scheme. A spectrophotometric method was used to compare the enzymatic activity of the rt-PA incorporated into ELIP (t-ELIP) to that of rt-PA. An in vitro flow model was employed to measure the thrombolytic efficacy and dose of ultraharmonic emissions from stable cavitation for 120-kHz ultrasound exposure of three treatment schemes: rt-PA, rt-PA and the perfluorocarbon-filled microbubble Definity(®), and t-ELIP. The enzymatic activity of rt-PA incorporated into t-ELIP was 28 % that of rt-PA. Thrombolytic efficacy of t-ELIP or rt-PA and Definity(®) was equivalent when the dose of t-ELIP was adjusted to produce comparable enzymatic activity. Sustained bubble activity was nucleated from Definity but not from t-ELIP exposed to 120-kHz ultrasound. These results emphasize the advantages of encapsulating a thrombolytic and the importance of incorporating an insoluble gas required to promote sustained, stable cavitation activity.

  16. Effects of a Classroom-Based Physical Activity Program on Children's Physical Activity Levels

    ERIC Educational Resources Information Center

    Goh, Tan Leng; Hannon, James; Webster, Collin Andrew; Podlog, Leslie William; Brusseau, Timothy; Newton, Maria

    2014-01-01

    High levels of physical inactivity are evident among many American children. To address this problem, providing physical activity (PA) during the school day within the CSPAP framework, is one strategy to increase children's PA. Thus, the purpose of this study was to examine the effects of a classroom-based PA program on children's PA. Two hundred…

  17. Plasma tPA-Activity and Progression of Cerebral White Matter Hyperintensities in Lacunar Stroke Patients

    PubMed Central

    van Overbeek, Ellen C.; Staals, Julie; Knottnerus, Iris L. H.; ten Cate, Hugo; van Oostenbrugge, Robert J.

    2016-01-01

    Introduction Tissue plasminogen activator (tPA)-activity and plasminogen activator inhibitor type 1 (PAI-1) antigen are considered to be haemostasis-related markers of endothelial activation and relate to presence of cerebral white matter hyperintensities (WMH) as was earlier shown in a cross-sectional study. We investigated whether tPA-activity and PAI-1 levels are associated with WMH progression in a longitudinal study. Methods In 127 first-ever lacunar stroke patients in whom baseline brain MRI and plasma levels of tPA-activity and PAI-1-antigen were available, we obtained a 2-year follow-up MRI. We assessed WMH progression by a visual WMH change scale. We determined the relationship between levels of tPA-activity and PAI-1 and WMH progression, by logistic regression analysis. Results Plasma tPA-activity was associated with periventricular WMH progression (OR 2.36, 95% CI 1.01–5.49, with correction for age and sex and baseline presence of WMH), but not with deep or any (periventricular and/or deep) WMH progression. PAI-1 levels were lower in patients with WMH progression, but these results were not significant. Conclusion We found a relationship between plasma tPA-activity and progression of periventricular WMH. More research is needed to determine whether there is a (direct) role of tPA in the development and progression of WMH. PMID:26942412

  18. The Association between Socio-Ecological Factors and Having an After-School Physical Activity Program

    ERIC Educational Resources Information Center

    Van Acker, Ragnar; De Bourdeaudhuij, Ilse; De Martelaer, Kristine; Seghers, Jan; De Cocker, Katrien; Cardon, Greet

    2012-01-01

    Background: After-school physical activity (PA) programs promote PA among youth. Few studies have used socio-ecological health models to identify barriers and facilitators of after-school PA programs. This study examined which socio-ecological factors are associated with having an after-school PA program. Methods: A questionnaire was administered…

  19. Impact of an After-School Physical Activity Program on Youth's Physical Activity Correlates and Behavior

    ERIC Educational Resources Information Center

    Huang, Chaoqun; Gao, Zan; Hannon, James C.; Schultz, Barry; Newton, Maria; Jenson, William

    2012-01-01

    The purpose of this investigation was to examine the effect of a sports-based, after-school physical activity (PA) program on youth's physical activity PA levels and PA correlates. After the pretest, 130 youth were assigned to the intervention group (i.e., after-school PA group) or the comparison (i.e., no after-school PA group) group.…

  20. Molecular and functional characterization of a putative PA28γ proteasome activator orthologue in Schistosoma mansoni.

    PubMed

    Soares, Cláudia Sossai; Morais, Enyara Rezende; Magalhães, Lizandra G; Machado, Carla Botelho; Moreira, Érika Bueno de Carvalho; Teixeira, Felipe Roberti; Rodrigues, Vanderlei; Yoshino, Timothy P

    2013-05-01

    PA28γ is a proteasome activator involved in the regulation of the cellular proliferation, differentiation and growth. In the present study, we identified and characterized a cDNA from Schistosoma mansoni exhibiting significant homology to PA28γ of diverse taxa ranging from mammals (including humans) to simple invertebrates. Designated SmPA28γ, this transcript has a 753bp predicted ORF encoding a protein of 250 amino acid residues. Alignment of SmPA28γ with multiple PA28γ orthologues revealed an average similarity of ~40% among the investigated organisms, and 90% similarity with PA28γ from Schistosoma japonicum. In addition, phylogenetic analysis demonstrated a close linkage between SmPA28γ to its sister group that contains well-characterized PA28γ sequences from Drosophila spp., as well as sharing the same branch with PA28γ from S. japonicum. Gene expression profiling of SmPA28γ using real-time quantitative PCR revealed elevated steady-state transcript levels in the eggs, miracidia and paired adult worms compared to other stages. In parallel with gene expression profiles, an affinity-purified anti-SmPA28γ antibody produced against recombinant protein exhibited strongest reactivity in Western blot analyses to endogenous SmPA28γ from miracidia, sporocysts and paired adult worms. Given its known regulatory function in other organisms, we hypothesized that the high level of SmPA28γ transcript and protein in these stages may be correlated with an important role of the PA28γ in the cellular growth and/or development of this parasite. To address this hypothesis, miracidia were transformed in vitro to sporocysts in the presence of SmPA28γ double-stranded RNAs (dsRNAs) and cultivated for 4 days, after which time steady-state transcript and protein levels, and phenotypic changes were evaluated. SmPA28γ dsRNA treatment resulted in gene and protein knockdown of ~60% and ~80%, respectively, which were correlated with a significant decrease in larval length

  1. Inhibition of PA endonuclease activity of influenza virus RNA polymerase by Kampo medicines.

    PubMed

    Shirayama, Riku; Shoji, Masaki; Sriwilaijaroen, Nongluk; Hiramatsu, Hiroaki; Suzuki, Yasuo; Kuzuhara, Takashi

    To find a novel influenza inhibitor targeting the endonuclease activity of influenza A virus polymerase acidic protein (PA), which is essential for the acquisition of primers for viral mRNA transcription, seven Kampo extracts were tested in vitro for their ability to inhibit endonuclease activity of the recombinant PA protein that was expressed and purified from Escherichia coli. The Kampo medicines Kakkonto, Shosaikoto, Saikokeishito, Keishito, Maobushisaishinto, and Maoto, but not Chikujountanto, inhibited PA endonuclease activity in a dose-dependent manner. Our results indicate that Kampo medicines are good sources providing a structural lead for optimization of an influenza endonuclease inhibitor.

  2. A novel plasminogen activator from Agkistrodon blomhoffii Ussurensis venom (ABUSV-PA): Purification and characterization

    SciTech Connect

    Liu Shuqing; Sun Mingzhong . E-mail: ming-zhong.sun@case.edu; Greenaway, Frederick T.

    2006-10-06

    A plasminogen activator with arginine ester hydrolysis activity (ABUSV-PA) has been identified and purified to homogeneity from Chinese Agkistrodon blomhoffii Ussurensis snake venom. ABUSV-PA, a monomeric protein with molecular mass of 27815.2 Da, was purified 180-fold with 0.02% recovery for protein and 3.6% recovery for esterase activity. ABUSV-PA reacts optimally with its substrate N {sub {alpha}}-tosyl-L-arginine-methyl ester (TAME) at {approx}pH 7.5 and at 51 {sup o}C. Measurement from inductively coupled plasma-atomic emission spectroscopy (ICP-AES) reveals that ABUSV-PA is a Zn{sup 2+}-containing protein with a stoichiometry of 1:1 [Zn{sup 2+}]:[ABUSV-PA]. Analyses of esterase hydrolysis and UV absorption and CD spectra indicate that Zn{sup 2+} plays an important role in maintaining the structural integrity rather than the esterase activity of ABUSV-PA. Divalent metal ions, including Ca{sup 2+}, Mg{sup 2+}, Cu{sup 2+}, Ni{sup 2+}, Mn{sup 2+}, and Co{sup 2+}, increase the TAME hydrolysis activity of ABUSV-PA. A red-shift of the emission wavelengths of the synchronous fluorescence of ABUSV-PA, compared to those of free Tyr and Trp, indicates a conformation where the Tyr and Trp residues are in exposed hydrophilic environments. The presence of zinc increases the hydrophobicity of the conformational environments surrounding the Trp residues of ABUSV-PA and affects the secondary structure of ABUSV-PA, as proved by UV absorption and CD spectroscopy.

  3. Parents' and Children's Perceptions of the Keep It Moving! After-School Physical Activity Program

    ERIC Educational Resources Information Center

    Behrens, Timothy K.; Wegner, Rebekah L.; Miller, Daniel J.; Liebert, Mina L.; Smith, Jennifer Howard

    2015-01-01

    After-school PA programs have been used as an outlet to help children increase PA levels. To attract children and their parents, it is important to understand perceptions about programs. With child and parent input, researchers and practitioners will better be able to increase PA with activities the children enjoy and encourage increased PA. A…

  4. Impacts of tissue-type plasminogen activator (tPA) on neuronal survival

    PubMed Central

    Chevilley, Arnaud; Lesept, Flavie; Lenoir, Sophie; Ali, Carine; Parcq, Jérôme; Vivien, Denis

    2015-01-01

    Tissue-type plasminogen activator (tPA) a serine protease is constituted of five functional domains through which it interacts with different substrates, binding proteins, and receptors. In the last years, great interest has been given to the clinical relevance of targeting tPA in different diseases of the central nervous system, in particular stroke. Among its reported functions in the central nervous system, tPA displays both neurotrophic and neurotoxic effects. How can the protease mediate such opposite functions remain unclear but several hypotheses have been proposed. These include an influence of the degree of maturity and/or the type of neurons, of the level of tPA, of its origin (endogenous or exogenous) or of its form (single chain tPA versus two chain tPA). In this review, we will provide a synthetic snapshot of our current knowledge regarding the natural history of tPA and discuss how it sustains its pleiotropic functions with focus on excitotoxic/ischemic neuronal death and neuronal survival. PMID:26528141

  5. Labeling of human clots in vitro with an active-site mutant of t-PA

    SciTech Connect

    Fry, E.T.; Mack, D.L.; Monge, J.C.; Billadello, J.J.; Sobel, B.E. )

    1990-02-01

    Prompt detection of acute thrombosis and its response to treatment with thrombolytic agents generally require angiography. Scintigraphic approaches with labeled antibodies to or components of the coagulation and fibrinolytic systems have been disappointing because of prolonged circulating half-lives of tracers and relatively slow or limited binding to thrombi. Accordingly, we developed and characterized a thrombolytically inactive, active-site mutant (Ser-478----Thr) of tissue-type plasminogen activator (t-PA) designed to detect thrombi in vivo. Binding of iodine-125-({sup 125}I) labeled Ser----Thr t-PA to thrombi in vitro was time- and concentration-dependent, and specific judging from inhibition by pre-incubation with anti-t-PA IgG. Clearance of 125I-labeled mutant t-PA in rabbits was rapid and biexponential (alpha t1/2 = 1.9 +/- 0.4 min, beta t1/2 = 39.8 +/- 11.2 min). Thus, the amidolytically inactive mutant of t-PA designed binds rapidly and specifically to human thrombi in vitro and is cleared rapidly from the circulation in vivo--properties rendering it attractive as a potentially useful clot imaging agent.

  6. Physical Activity Change through Comprehensive School Physical Activity Programs in Urban Elementary Schools

    ERIC Educational Resources Information Center

    Centeio, Erin E.; McCaughtry, Nate; Gutuskey, Lila; Garn, Alex C.; Somers, Cheryl; Shen, Bo; Martin, Jeffrey J.; Kulik, Noel L.

    2014-01-01

    The impact of Comprehensive School Physical Activity Programs (CSPAPs) on urban children's, educators', and parents' physical activity (PA) is relatively unknown. The purpose of this study was to explore overall changes in student, educator, and parent PA after an 8-month CSPAP-based program. This longitudinal, exploratory study implemented a…

  7. Psychological correlates of self-reported and objectively measured physical activity among Chinese children—psychological correlates of PA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study aimed to explore the associations among psychological correlates and physical activity (PA) in Chinese children and to further examine whether these associations varied by different PA measures. PA self-efficacy, motivation, and preference were reported in 449 8–13-year-old Chinese childr...

  8. PA28, an activator of the 20 S proteasome, is inactivated by proteolytic modification at its carboxyl terminus.

    PubMed

    Ma, C P; Willy, P J; Slaughter, C A; DeMartino, G N

    1993-10-25

    PA28, a protein activator of the 20 S proteasome, was previously identified in soluble extracts of bovine red blood cells (Ma, C.-P., Slaughter, C. A., and DeMartino, G. N. (1992) J. Biol. Chem. 267, 10515-10523). To determine whether this regulatory protein is as widely distributed as the proteasome, PA28 content and activity were examined in various eukaryotic tissues by immunoblot analysis and by functional assays of tissue extracts. PA28 protein was present in all sources examined. PA28 activity, however, was not detected in many of these sources, including those with the highest level of PA28 protein. To determine the biochemical basis of this result, PA28 was purified from extracts of rat liver, which had high levels of PA28 protein but no PA28 activity. The resulting purified PA28 had no detectable activity but had native and subunit molecular weights indistinguishable from the active PA28 of bovine red blood cells. Using the inactivation of purified PA28 as an assay, a protein that inactivated PA28 without altering its apparent molecular weight on SDS-polyacrylamide gel electrophoresis was identified, purified, and characterized from bovine liver. It had biochemical and catalytic characteristics similar to those of lysosomal carboxypeptidase B. When leupeptin, an inhibitor of lysosomal carboxypeptidase B, was included in the buffers used for the preparation of PA28, PA28 activity was detected in tissues which otherwise failed to demonstrate this activity. A similar result was obtained when extracts were prepared in a manner that minimized disruption of lysosomes. Other carboxypeptidases such as carboxypeptidase Y and pancreatic carboxypeptidase B also inactivated PA28 without altering its apparent molecular weight. Active PA28 binds to the proteasome to form a protease-activator complex that can be isolated after velocity sedimentation centrifugation through glycerol density gradients. Carboxypeptidase-inactivated PA28 failed to form such a complex

  9. Pyruvate and cilostazol protect cultured rat cortical pericytes against tissue plasminogen activator (tPA)-induced cell death.

    PubMed

    Kim, Ha Na; Kim, Tae-Youn; Yoon, Young Hee; Koh, Jae-Young

    2015-12-02

    Since even a brief ischemia can cause permanent brain damage, rapid restoration of blood flow is critical to limiting damage. Although intravenous tPA during the acute stage is the treatment of choice for achieving reperfusion, this treatment is sometimes associated with brain hemorrhage. Agents that reduce tPA-related bleeding risk may help expand its therapeutic window. This study assessed whether zinc dyshomeostasis underlies the toxic effect of tPA on brain vascular pericytes; whether pyruvate, an inhibitor of zinc toxicity, protects pericytes against tPA-induced cell death; and whether cilostazol, which protects pericytes against tPA-induced cell death, affects zinc dyshomeostasis associated with tPA toxicity. Cultured pericytes from newborn rat brains were treated with 10-200 μg/ml tPA for 24 h, inducing cell death in a concentration-dependent manner. tPA-induced cell death was preceded by increases in intracellular free zinc levels, and was substantially attenuated by plasminogen activator inhibitor-1 (PAI-1) or TPEN. Pyruvate completely blocked direct zinc toxicity and tPA-induced pericyte cell death. Both cAMP and cilostazol, a PDE3 inhibitor that attenuates tPA-induced pericyte cell death in vitro and tPA-induced brain hemorrhage in vivo, reduced zinc- and tPA-induced pericyte cell death, suggesting that zinc dyshomeostasis may be targeted by cilostazol in tPA toxicity. These findings show that tPA-induced pericyte cell death may involve zinc dyshomeostasis, and that pyruvate and cilostazol attenuate tPA-induced cell death by reducing the toxic cascade triggered by zinc dyshomeostasis. Since pyruvate is an endogenous metabolite and cilostazol is an FDA-approved drug, in vivo testing of both as protectors against tPA-induced brain hemorrhage may be warranted. This article is part of a Special Issue entitled SI: Neuroprotection.

  10. An Objective Assessment of Children's Physical Activity during the Keep It Moving! After-School Program

    ERIC Educational Resources Information Center

    Schuna, John M., Jr.; Lauersdorf, Rebekah L.; Behrens, Timothy K.; Liguori, Gary; Liebert, Mina L.

    2013-01-01

    Background: After-school programs may provide valuable opportunities for children to accumulate healthful physical activity (PA). This study assessed the PA of third-, fourth-, and ?fth-grade children in the Keep It Moving! (KIM) after-school PA program, which was implemented in an ethnically diverse and low socioeconomic status school district in…

  11. Gene therapy by proteasome activator, PA28γ, improves motor coordination and proteasome function in Huntington's disease YAC128 mice.

    PubMed

    Jeon, J; Kim, W; Jang, J; Isacson, O; Seo, H

    2016-06-02

    Huntington's disease (HD) is neurologically characterized by involuntary movements, associated with degeneration of the medium-sized spiny neurons (MSNs) and ubiquitin-positive neuronal intranuclear inclusions (NIIs). It has been reported that the proteolytic activities of the ubiquitin-proteasome system (UPS) are generally inhibited in HD patient's brain. We previously discovered that a proteasome activator (PA), PA28γ enhances proteasome activities and cell survival in in vitro HD model. In this study, we aimed to find whether PA28γ gene transfer improves the proteasome activities and pathological symptoms in in vivo HD model. We stereotaxically injected lenti-PA28γ virus into the striatum of mutant (MT) YAC128 HD mice and littermate (LM) controls at 14-18months of age, and validated their behavioral and biochemical changes at 12weeks after the injection. YAC128 mice showed a significant increase in their peptidyl-glutamyl preferring hydrolytic (PGPH) proteasome activity and the mRNA or protein levels of brain-derived neurotrophic factor (BDNF) and pro-BDNF after lenti-PA28γ injection. The number of ubiquitin-positive inclusion bodies was reduced in the striatum of YAC128 mice after lenti-PA28γ injection. YAC128 mice showed significant improvement of latency to fall on the rota-rod test after lenti-PA28γ injection. These data demonstrate that the gene therapy with PA, PA28γ can improve UPS function as well as behavioral abnormalities in HD model mice.

  12. Active Fire Mapping Program

    MedlinePlus

    Active Fire Mapping Program Current Large Incidents (Home) New Large Incidents Fire Detection Maps MODIS Satellite Imagery VIIRS Satellite Imagery Fire Detection GIS Data Fire Data in Google Earth ...

  13. Expression and Biological Activity of the Cystine Knot Bioinsecticide PA1b (Pea Albumin 1 Subunit b)

    PubMed Central

    Eyraud, Vanessa; Karaki, Lamis; Rahioui, Isabelle; Sivignon, Catherine; Da Silva, Pedro; Rahbé, Yvan; Royer, Corinne; Gressent, Frédéric

    2013-01-01

    The PA1b (Pea Albumin 1, subunit b) peptide is an entomotoxin extract from Legume seeds with lethal activity on several insect pests, such as mosquitoes, some aphids and cereal weevils. This 37 amino-acid cysteine-rich peptide has been, until now, obtained by biochemical purification or chemical synthesis. In this paper, we present our results for the transient production of the peptide in Nicotiana benthamiana by agro-infiltration, with a yield of about 35 µg/g of fresh leaves and maximum production 8 days after infiltration. PA1b is part of the PA1 gene which, after post-translational modifications, encodes two peptides (PA1b and PA1a). We show that transforming tobacco with the PA1b cDNA alone does not result in production of the toxin and, in fact, the entire cDNA is necessary, raising the question of the role of PA1a. We constructed a PA1-cassette, allowing for the quick “cut/paste” of different PA1b mutants within a conserved PA1 cDNA. This cassette enabled us to produce the six isoforms of PA1b which exist in pea seeds. Biological tests revealed that all the isoforms display similar activity, with the exception of one which is inactive. The lack of activity in this isoform led us to conclude that the amphiphilic nature of the peptide is necessary for activity. The possible applications of this expression system for other cysteine-rich biomolecules are discussed. PMID:24349099

  14. Expression and biological activity of the cystine knot bioinsecticide PA1b (Pea Albumin 1 Subunit b).

    PubMed

    Eyraud, Vanessa; Karaki, Lamis; Rahioui, Isabelle; Sivignon, Catherine; Da Silva, Pedro; Rahbé, Yvan; Royer, Corinne; Gressent, Frédéric

    2013-01-01

    The PA1b (Pea Albumin 1, subunit b) peptide is an entomotoxin extract from Legume seeds with lethal activity on several insect pests, such as mosquitoes, some aphids and cereal weevils. This 37 amino-acid cysteine-rich peptide has been, until now, obtained by biochemical purification or chemical synthesis. In this paper, we present our results for the transient production of the peptide in Nicotiana benthamiana by agro-infiltration, with a yield of about 35 µg/g of fresh leaves and maximum production 8 days after infiltration. PA1b is part of the PA1 gene which, after post-translational modifications, encodes two peptides (PA1b and PA1a). We show that transforming tobacco with the PA1b cDNA alone does not result in production of the toxin and, in fact, the entire cDNA is necessary, raising the question of the role of PA1a. We constructed a PA1-cassette, allowing for the quick "cut/paste" of different PA1b mutants within a conserved PA1 cDNA. This cassette enabled us to produce the six isoforms of PA1b which exist in pea seeds. Biological tests revealed that all the isoforms display similar activity, with the exception of one which is inactive. The lack of activity in this isoform led us to conclude that the amphiphilic nature of the peptide is necessary for activity. The possible applications of this expression system for other cysteine-rich biomolecules are discussed.

  15. Psychological Correlates of Self-Reported and Objectively Measured Physical Activity among Chinese Children—Psychological Correlates of PA

    PubMed Central

    Wang, Jing-Jing; Baranowski, Tom; Lau, Patrick W. C.; Chen, Tzu-An; Zhang, Shu-Ge

    2016-01-01

    This study aimed to explore the associations among psychological correlates and physical activity (PA) in Chinese children and to further examine whether these associations varied by different PA measures. PA self-efficacy, motivation, and preference were reported in 449 8–13-year-old Chinese children (252 males). Moderate- to vigorous- intensity PA (MVPA) was measured by the Physical Activity Questionnaire for Older Children (PAQ-C) and with an ActiGraph GT3X accelerometer. Correlations and hierarchical regressions were performed to explore their associations. The study psychological variables were all positively related to PAQ-C and objective MVPA (r: 0.22–0.63). The associations with PAQ-C were all substantially stronger than those with accelerometry. Beyond the explained variance accounted for by demographics and social desirability, the addition of the psychological correlates accounted for 45% of the variance of the PAQ-C score, while only 13% for accelerometry-based MVPA. The associations of specific variables with the PAQ-C score (age, PA self-efficacy, autonomous motivation and preference) were somewhat different from those associated with objective MVPA (PA self-efficacy, autonomous motivation, and negatively associated with female gender). This study demonstrated the importance of self-efficacy and autonomous motivation in association with PA and indicated the difference in level of their associations with different PA measures. PMID:27754396

  16. Induction of primary cutaneous melanocytic neoplasms in urokinase-type plasminogen activator (uPA)-deficient and wild-type mice: cellular blue nevi invade but do not progress to malignant melanoma in uPA-deficient animals.

    PubMed

    Shapiro, R L; Duquette, J G; Roses, D F; Nunes, I; Harris, M N; Kamino, H; Wilson, E L; Rifkin, D B

    1996-08-01

    Evidence suggests that the plasminogen activators (PAs), in particular urokinase-type PA (uPA), play a pivotal role in tumor invasion and metastasis. We studied the contribution of the PAs to the malignant phenotype through the chemical induction of melanocytic neoplasms in uPA-deficient mice. Primary tumors were induced and promoted concurrently in 35 uPA-/- deficient and 35 uPA+/+ wild-type mice using a single application of 7,12-dimethylbenz(a)anthracene followed by repetitive applications of croton oil. Animals were sacrificed at 60-day intervals for 1 year. At necropsy, the four largest pigmented lesions in each animal were excised, characterized histologically, and evaluated microscopically for evidence of invasion. The regional lymph nodes, lungs, and solid abdominal visceral organs were sectioned and examined microscopically for evidence of metastatic disease. Cellular blue nevi were induced in 100% of uPA-/- and uPA+/+ promoted animals. Although a reduction in the radial and vertical progression of these lesions was noted in the uPA-deficient mice compared with the wild-type group, more than 95% of cellular blue nevi induced in both groups of animals invaded the underlying tissues. These lesions did not metastasize to the regional lymph nodes. Malignant melanoma arose in 5 of 35 (14.3%) of promoted wild-type mice. These tumors were locally aggressive, produced tissue-type PA, but were not metastatic to the regional nodes, lungs, or abdominal viscera. These results indicate that the invasive capability of melanocytic lesions may depend more on tissue-type PA than uPA activity. No melanomas were induced in the uPA-/- mice. The resistance of the uPA -/- strain to melanoma induction suggests that uPA contributes to malignant progression. We propose that the absence of uPA negatively affects tumorigenesis by decreasing the liberation and availability of growth factors such as basic fibroblast growth factor.

  17. Spatial arrangement and functional role of α subunits of proteasome activator PA28 in hetero-oligomeric form

    SciTech Connect

    Sugiyama, Masaaki; Sahashi, Hiroki; Kurimoto, Eiji; Takata, Shin-ichi; Yagi, Hirokazu; Kanai, Keita; Sakata, Eri; Minami, Yasufumi; Tanaka, Keiji; Kato, Koichi

    2013-03-01

    Highlights: ► Homologous α and β subunits are alternatively arranged in the PA28 heptameric ring. ► The flexible loops of the three α subunits surround the site of substrate entry. ► The loops serve as gatekeepers that selectively hinder passage of longer peptides. - Abstract: A major form of proteasome activator PA28 is a heteroheptamer composed of interferon-γ-inducible α and β subunits, which share approximately 50% amino acid identity and possess distinct insert loops. This activator forms a complex with the 20S proteasome and thereby stimulates proteasomal degradation of peptides in an ATP-independent manner, giving rise to smaller antigenic peptides presented by major histocompatibility complex class I molecules. In this study, we performed biophysical and biochemical characterization of the structure and function of the PA28 hetero-oligomer. Deuteration-assisted small-angle neutron scattering demonstrated three α and four β subunits are alternately arranged in the heptameric ring. In this arrangement, PA28 loops surround the central pore of the heptameric ring (site for peptide entry). Activating the 20S proteasome with a PA28 mutant that lacked the α subunit loops cleaved model substrates longer than a nonapeptide with better efficiency when compared to wild-type PA28. Based on these data, we hypothesize that the flexible PA28 loops act as gatekeepers, which function to select the length of peptide substrates to be transported between the proteolytic chamber and the extra-proteasomal medium.

  18. Scale up and pharmacokinetic study of a novel mutated chimeric tissue plasminogen activator (mt-PA) in rats

    PubMed Central

    Raigani, Mozhgan; Rouini, Mohammad-Reza; Golabchifar, Ali-Akbar; Mirabzadeh, Esmat; Vaziri, Behrouz; Barkhordari, Farzaneh; Davami, Fatemeh; Mahboudi, Fereidoun

    2017-01-01

    Because of high mortality caused by cardiovascular diseases, various fibrinolytic agents with diverse pharmacokinetic and pharmacodynamic properties have been developed. A novel mutated chimeric tissue plasminogen activator (mt-PA) was developed by the removal of first three domains of t-PA, insertion of GHRP sequence and mutation towards resistance to plasminogen activator inhibitor-1 (PAI-1). Mt-PA protein was expressed in Expi293F cells. The expression level of mt-PA was found to be 5000 IU/mL. Following purification, the pharmacokinetic properties of mt-PA were evaluated in three doses in rats. Data related to mt-PA were best fitted to two compartment model. With the increase in dose, the Area Under the plasma concentration-time Curve (AUC0→∞) increased. The elimination half-life (t1/2) of mt-PA was in the range of 19.1–26.1 min in three doses while that of Alteplase was 8.3 min. The plasma clearance (CLp) of mt-PA ranged from 3.8 to 5.9 mL/min in three doses, which was several times lower than that of Alteplase (142.6 mL/min). The mean residence time (MRT) of mt-PA ranged from 23.3–31.8 min in three doses, which was 4–5 times greater than that of Alteplase (6 min). Mt-PA showed extended half-life and mean residence time and is a good candidate for further clinical studies. PMID:28223717

  19. Physical and Social-Motivational Contextual Correlates of Youth Physical Activity in Underresourced Afterschool Programs

    ERIC Educational Resources Information Center

    Zarrett, Nicole; Sorensen, Carl; Cook, Brittany Skiles

    2015-01-01

    Afterschool programs (ASPs) have become increasingly recognized as a key context to support youth daily physical activity (PA) accrual. The purpose of the present study was to assess the physical and social-motivational climate characteristics of ASPs associated with youth PA, and variations in contextual correlates of PA by youth sex. Systematic…

  20. Psychological Changes among Muslim Students Participating in a Faith-Based School Physical Activity Program

    ERIC Educational Resources Information Center

    Nicaise, Virginie; Kahan, David

    2013-01-01

    Purpose: Some religions espouse doctrines that (in)directly impact physical activity (PA) behavior. Yet limited PA interventions have been tailored to religious minorities. Thus, a formative study was conducted to examine the effect of a faith-based pedometer program (Virtual Umra) on psychological correlates of PA behavior and their contribution…

  1. Multifunctional roles of urokinase plasminogen activator (uPA) in cancer stemness and chemoresistance of pancreatic cancer

    PubMed Central

    Asuthkar, Swapna; Stepanova, Victoria; Lebedeva, Tatiana; Holterman, AiXuan L.; Estes, Norman; Cines, Douglas B.; Rao, Jasti S.; Gondi, Christopher S.

    2013-01-01

    Pancreatic ductal adenocarcinoma (PDAC) is almost always lethal. One of the underlying reasons for this lethality is believed to be the presence of cancer stem cells (CSC), which impart chemoresistance and promote recurrence, but the mechanisms responsible are unclear. Recently the poor prognosis of PDAC has been correlated with increased expression of urokinase plasminogen activator (uPA). In the present study we examine the role of uPA in the generation of PDAC CSC. We observe a subset of cells identifiable as a side population (SP) when sorted by flow cytometry of MIA PaCa-2 and PANC-1 pancreatic cancer cells that possess the properties of CSC. A large fraction of these SP cells are CD44 and CD24 positive, are gemcitabine resistant, possess sphere-forming ability, and exhibit increased tumorigenicity, known characteristics of cancer stemness. Increased tumorigenicity and gemcitabine resistance decrease after suppression of uPA. We observe that uPA interacts directly with transcription factors LIM homeobox-2 (Lhx2), homeobox transcription factor A5 (HOXA5), and Hey to possibly promote cancer stemness. uPA regulates Lhx2 expression by suppressing expression of miR-124 and p53 expression by repressing its promoter by inactivating HOXA5. These results demonstrate that regulation of gene transcription by uPA contributes to cancer stemness and clinical lethality. PMID:23864708

  2. Multifunctional roles of urokinase plasminogen activator (uPA) in cancer stemness and chemoresistance of pancreatic cancer.

    PubMed

    Asuthkar, Swapna; Stepanova, Victoria; Lebedeva, Tatiana; Holterman, Aixuan L; Estes, Norman; Cines, Douglas B; Rao, Jasti S; Gondi, Christopher S

    2013-09-01

    Pancreatic ductal adenocarcinoma (PDAC) is almost always lethal. One of the underlying reasons for this lethality is believed to be the presence of cancer stem cells (CSC), which impart chemoresistance and promote recurrence, but the mechanisms responsible are unclear. Recently the poor prognosis of PDAC has been correlated with increased expression of urokinase plasminogen activator (uPA). In the present study we examine the role of uPA in the generation of PDAC CSC. We observe a subset of cells identifiable as a side population (SP) when sorted by flow cytometry of MIA PaCa-2 and PANC-1 pancreatic cancer cells that possess the properties of CSC. A large fraction of these SP cells are CD44 and CD24 positive, are gemcitabine resistant, possess sphere-forming ability, and exhibit increased tumorigenicity, known characteristics of cancer stemness. Increased tumorigenicity and gemcitabine resistance decrease after suppression of uPA. We observe that uPA interacts directly with transcription factors LIM homeobox-2 (Lhx2), homeobox transcription factor A5 (HOXA5), and Hey to possibly promote cancer stemness. uPA regulates Lhx2 expression by suppressing expression of miR-124 and p53 expression by repressing its promoter by inactivating HOXA5. These results demonstrate that regulation of gene transcription by uPA contributes to cancer stemness and clinical lethality.

  3. Promoting Health-Related Fitness for Elementary Students with Intellectual Disabilities through a Specifically Designed Activity Program

    ERIC Educational Resources Information Center

    Davis, Kathryn; Zhang, Guili; Hodson, Patricia

    2011-01-01

    The Motivate, Adapt, and Play Program was specifically designed as an adapted physical activity (PA) program for students with intellectual disabilities (ID) to meet required school PA policies to combat childhood obesity. The policies commonly require a minimum of 30 min of PA per school day. A study was undertaken to test the efficacy of the…

  4. Recombinant tissue plasminogen activator (rtPA) for the treatment of hepatic veno-occlusive disease (VOD).

    PubMed

    Kulkarni, S; Rodriguez, M; Lafuente, A; Mateos, P; Mehta, J; Singhal, S; Saso, R; Tait, D; Treleaven, J G; Powles, R L

    1999-04-01

    Seventeen patients who developed hepatic veno-occlusive disease (VOD) following hematopoietic stem cell transplantation were treated with recombinant tissue plasminogen activator (rtPA) with or without heparin. rtPA was started a median of 13 days post transplant (range 4-35). All patients received rtPA at a dose of 10 mg/day as a starting dose, and 12 patients also received heparin (1500 U bolus; then 100 U/kg/day as a continuous i.v. infusion). The median number of days of rtPA therapy was 2.5 (1-12). The median total serum bilirubin level was 116 mmol/l (range 63-194) at the beginning of treatment. Six patients showed a response to rtPA treatment (29%). It was observed that by day 2 of rtPA therapy, bilirubin levels in responders showed a downwards trend as compared to those in nonresponders. In all except one patient this response was observed after two doses of rtPA. Seven out of the 11 non-responders had a past history of liver dysfunction, compared with none of the responders. There were no differences between the two groups in terms of day of onset of liver dysfunction, manifestations of disease, maximum bilirubin and creatinine levels, and day of commencing treatment. No patient experienced severe hemorrhagic complications during therapy. Four responders survived for more than 100 days compared to none of the non-responders. Probability of survival was 33% at day 100. It is difficult to unequivocally establish the role of rtPA in the treatment of VOD. The importance of bilirubin levels on days 2 or 3 of therapy in predicting outcome should be established, as should the optimum dose of rtPA and optimum duration of therapy.

  5. 77 FR 43136 - Approval of Noise Compatibility Program for Philadelphia International Airport, Philadelphia, PA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-23

    ... Program which sets forth the measures taken or proposed by the airport operator for the reduction of... with respect to which measures should be recommended for action. The FAA's approval or disapproval of Part 150 program recommendations is measured according to the standards expressed in Part 150 and...

  6. Geochemical data package for the Hanford immobilized low-activity tank waste performance assessment (ILAW PA)

    SciTech Connect

    DI Kaplan; RJ Serne

    2000-02-24

    Lockheed Martin Hanford Company (LMHC) is designing and assessing the performance of disposal facilities to receive radioactive wastes that are stored in single- and double-shell tanks at the Hanford Site. The preferred method of disposing of the portion that is classified as low-activity waste is to vitrify the liquid/slurry and place the solid product in near-surface, shallow-land burial facilities. The LMHC project to assess the performance of these disposal facilities is the Hanford Immobilized Low-Activity Tank Waste (ILAW) Performance Assessment (PA) activity. The goal of this project is to provide a reasonable expectation that the disposal of the waste is protective of the general public, groundwater resources, air resources, surface-water resources, and inadvertent intruders. Achieving this goal will require prediction of contaminant migration from the facilities. This migration is expected to occur primarily via the movement of water through the facilities, and the consequent transport of dissolved contaminants in the porewater of the vadose zone. Pacific Northwest National Laboratory assists LMHC in their performance assessment activities. One of the PNNL tasks is to provide estimates of the geochemical properties of the materials comprising the disposal facility, the disturbed region around the facility, and the physically undisturbed sediments below the facility (including the vadose zone sediments and the aquifer sediments in the upper unconfined aquifer). The geochemical properties are expressed as parameters that quantify the adsorption of contaminants and the solubility constraints that might apply for those contaminants that may exceed solubility constraints. The common parameters used to quantify adsorption and solubility are the distribution coefficient (K{sub d}) and the thermodynamic solubility product (K{sub sp}), respectively. In this data package, the authors approximate the solubility of contaminants using a more simplified construct

  7. A Framework for Physical Activity Programs within School-Community Partnerships

    ERIC Educational Resources Information Center

    Van Acker, Ragnar; De Bourdeaudhuij, Ilse; De Martelaer, Kristine; Seghers, Jan; Kirk, David; Haerens, Leen; De Cocker, Katrien; Cardon, Greet

    2011-01-01

    School-community partnerships have shown their potential as incubators for innovations and for contributing to comprehensive physical activity (PA) programs. However, implementation frameworks for school-community partnerships that allow local tailoring of PA programs remain scarce. The present paper aims at documenting the composition of a…

  8. Barriers to and Facilitators of Physical Activity Program Use Among Older Adults

    PubMed Central

    Bethancourt, Hilary J.; Rosenberg, Dori E.; Beatty, Tara; Arterburn, David E.

    2014-01-01

    Objective Regular physical activity (PA) is important for maintaining long-term physical, cognitive, and emotional health. However, few older adults engage in routine PA, and even fewer take advantage of programs designed to enhance PA participation. Though most managed Medicare members have free access to the Silver Sneakers and EnhanceFitness PA programs, the vast majority of eligible seniors do not utilize these programs. The goal of this qualitative study was to better understand the barriers to and facilitators of PA and participation in PA programs among older adults. Design This was a qualitative study using focus group interviews. Setting Focus groups took place at three Group Health clinics in King County, Washington. Participants Fifty-two randomly selected Group Health Medicare members between the ages of 66 to 78 participated. Methods We conducted four focus groups with 13 participants each. Focus group discussions were audio-recorded, transcribed, and analyzed using an inductive thematic approach and a social-ecological framework. Results Men and women were nearly equally represented among the participants, and the sample was largely white (77%), well-educated (69% college graduates), and relatively physically active. Prominent barriers to PA and PA program participation were physical limitations due to health conditions or aging, lack of professional guidance, and inadequate distribution of information on available and appropriate PA options and programs. Facilitators included the motivation to maintain physical and mental health and access to affordable, convenient, and stimulating PA options. Conclusion Older adult populations may benefit from greater support and information from their providers and health care systems on how to safely and successfully improve or maintain PA levels through later adulthood. Efforts among health care systems to boost PA among older adults may need to consider patient-centered adjustments to current PA programs, as

  9. The proteasome activator 11 S REG (PA28) and class I antigen presentation.

    PubMed Central

    Rechsteiner, M; Realini, C; Ustrell, V

    2000-01-01

    There are two immune responses in vertebrates: humoral immunity is mediated by circulating antibodies, whereas cytotoxic T lymphocytes (CTL) confer cellular immunity. CTL lyse infected cells upon recognition of cell-surface MHC Class I molecules complexed with foreign peptides. The displayed peptides are produced in the cytosol by degradation of host proteins or proteins from intracellular pathogens that might be present. Proteasomes are cylindrical multisubunit proteases that generate many of the peptides eventually transferred to the cell surface for immune surveillance. In mammalian proteasomes, six active sites face a central chamber. As this chamber is sealed off from the enzyme's surface, there must be mechanisms to promote entry of substrates. Two protein complexes have been found to bind the ends of the proteasome and activate it. One of the activators is the 19 S regulatory complex of the 26 S proteasome; the other activator is '11 S REG' [Dubiel, Pratt, Ferrell and Rechsteiner (1992) J. Biol. Chem. 267, 22369-22377] or 'PA28' [Ma, Slaughter and DeMartino (1992) J. Biol. Chem. 267, 10515-10523]. During the past 7 years, our understanding of the structure of REG molecules has increased significantly, but much less is known about their biological functions. There are three REG subunits, namely alpha, beta and gamma. Recombinant REGalpha forms a ring-shaped heptamer of known crystal structure. 11 S REG is a heteroheptamer of alpha and beta subunits. REGgamma is also presumably a heptameric ring, and it is found in the nuclei of the nematode work Caenorhabditis elegans and higher organisms, where it may couple proteasomes to other nuclear components. REGalpha and REGbeta, which are abundant in vertebrate immune tissues, are located mostly in the cytoplasm. Synthesis of REG alpha and beta subunits is induced by interferon-gamma, and this has led to the prevalent hypothesis that REG alpha/beta hetero-oligomers play an important role in Class I antigen

  10. pA2 values for antagonists of platelet activating factor on aggregation of rabbit platelets.

    PubMed Central

    O'Donnell, S. R.; Barnett, C. J.

    1988-01-01

    1. The relative potencies, and equilibrium dissociation constants, for nine antagonists of platelet activating factor (Paf) have been determined on rabbit platelets (in diluted platelet-rich plasma (PRP)) in experiments in which the aggregatory response to Paf was measured. 2. Log concentration-response (% maximum) curves to Paf were obtained in the absence (controls) and presence of different concentrations of each Paf antagonist drug. The antagonists shifted the Paf curves to a higher concentration range and the slopes of the Schild plots, constructed from these data, suggested that the drugs were competitive antagonists of Paf. The slopes of the Schild plots for CV-3988 and SRI 63-119 were greater than 1. 3. The pA2 values (pKB values in parentheses) were: WEB 2086 7.31 (7.63); SRI 63-119 6.95; L-652,731 6.71 (6.73); BN 52021 6.38 (6.47); SRI 63-072 6.36 (6.43); CV-3988 5.87; 48740 RP 4.97 (5.07); ketotifen 4.94 (4.95); thiazinamium 4.73 (4.76). 4. This study provides, for the first time, some functional response data for Paf antagonists (pKB values) which are in an appropriate form for use in classifying putative Paf receptors. The study also provides the comparative potencies of these Paf antagonists in inhibiting Paf-induced platelet aggregation. WEB 2086 was the most potent of the drugs examined. PMID:3293683

  11. Improved Antimicrobial Activities of Synthetic-Hybrid Bacteriocins Designed from Enterocin E50-52 and Pediocin PA-1

    PubMed Central

    Tiwari, Santosh Kumar; Sutyak Noll, Katia; Cavera, Veronica L.

    2014-01-01

    Two hybrid bacteriocins, enterocin E50-52/pediocin PA-1 (EP) and pediocin PA-1/enterocin E50-52 (PE), were designed by combining the N terminus of enterocin E50-52 and the C terminus of pediocin PA-1 and by combining the C terminus of pediocin PA-1 and the N terminus of enterocin E50-52, respectively. Both hybrid bacteriocins showed reduced MICs compared to those of their natural counterparts. The MICs of hybrid PE and EP were 64- and 32-fold lower, respectively, than the MIC of pediocin PA-1 and 8- and 4-fold lower, respectively, than the MIC of enterocin E50-52. In this study, the effect of hybrid as well as wild-type (WT) bacteriocins on the transmembrane electrical potential (ΔΨ) and their ability to induce the efflux of intracellular ATP were investigated. Enterocin E50-52, pediocin PA-1, and hybrid bacteriocin PE were able to dissipate ΔΨ, but EP was unable to deplete this component. Both hybrid bacteriocins caused a loss of the intracellular concentration of ATP. EP, however, caused a faster efflux than PE and enterocin E50-52. Enterocin E50-52 and hybrids PE and EP were active against the Gram-positive and Gram-negative bacteria tested, such as Micrococcus luteus, Salmonella enterica serovar Enteritidis 20E1090, and Escherichia coli O157:H7. The hybrid bacteriocins designed and described herein are antimicrobial peptides with MICs lower those of their natural counterparts. Both hybrid peptides induce the loss of intracellular ATP and are capable of inhibiting Gram-negative bacteria, and PE dissipates the electrical potential. In this study, the MIC of hybrid bacteriocin PE decreased 64-fold compared to the MIC of its natural peptide counterpart, pediocin PA-1. Inhibition of Gram-negative pathogens confers an additional advantage for the application of these peptides in therapeutics. PMID:25527560

  12. Improved antimicrobial activities of synthetic-hybrid bacteriocins designed from enterocin E50-52 and pediocin PA-1.

    PubMed

    Tiwari, Santosh Kumar; Sutyak Noll, Katia; Cavera, Veronica L; Chikindas, Michael L

    2015-03-01

    Two hybrid bacteriocins, enterocin E50-52/pediocin PA-1 (EP) and pediocin PA-1/enterocin E50-52 (PE), were designed by combining the N terminus of enterocin E50-52 and the C terminus of pediocin PA-1 and by combining the C terminus of pediocin PA-1 and the N terminus of enterocin E50-52, respectively. Both hybrid bacteriocins showed reduced MICs compared to those of their natural counterparts. The MICs of hybrid PE and EP were 64- and 32-fold lower, respectively, than the MIC of pediocin PA-1 and 8- and 4-fold lower, respectively, than the MIC of enterocin E50-52. In this study, the effect of hybrid as well as wild-type (WT) bacteriocins on the transmembrane electrical potential (ΔΨ) and their ability to induce the efflux of intracellular ATP were investigated. Enterocin E50-52, pediocin PA-1, and hybrid bacteriocin PE were able to dissipate ΔΨ, but EP was unable to deplete this component. Both hybrid bacteriocins caused a loss of the intracellular concentration of ATP. EP, however, caused a faster efflux than PE and enterocin E50-52. Enterocin E50-52 and hybrids PE and EP were active against the Gram-positive and Gram-negative bacteria tested, such as Micrococcus luteus, Salmonella enterica serovar Enteritidis 20E1090, and Escherichia coli O157:H7. The hybrid bacteriocins designed and described herein are antimicrobial peptides with MICs lower those of their natural counterparts. Both hybrid peptides induce the loss of intracellular ATP and are capable of inhibiting Gram-negative bacteria, and PE dissipates the electrical potential. In this study, the MIC of hybrid bacteriocin PE decreased 64-fold compared to the MIC of its natural peptide counterpart, pediocin PA-1. Inhibition of Gram-negative pathogens confers an additional advantage for the application of these peptides in therapeutics.

  13. Physical Activity Intensity, Lesson Context, and Teacher Interactions during an Unstructured Afterschool Physical Activity Program

    ERIC Educational Resources Information Center

    Behrens, Timothy K.; Miller, Daniel J.; Schuna, John M.; Liebert, Mina L.

    2015-01-01

    Background: Afterschool programs are promising arenas to improve youth physical activity (PA) levels. During the school year for 2012-2013, 5 elementary schools from a low-socioeconomic status (SES) school district in southern Colorado participated in evaluation of the afterschool program entitled Keep It Moving! (KIM). Methods: In this…

  14. Impact of Policy Environment Characteristics on Physical Activity and Sedentary Behaviors of Children Attending Afterschool Programs

    ERIC Educational Resources Information Center

    Beets, Michael W.; Huberty, Jennifer; Beighle, Aaron; Moore, Justin B.; Webster, Collin; Ajja, Rahma; Weaver, Glenn

    2013-01-01

    State and national organizations recently developed policies focused on increasing physical activity (PA) in afterschool programs (ASPs). These policies emphasize "activity friendly" environment characteristics that, when present, should lead to higher levels of PA and reduce the amount of time children spend sedentary during an ASP.…

  15. Two-photon activation and excitation properties of PA-GFP in the 720-920-nm region.

    PubMed

    Schneider, Marc; Barozzi, Sara; Testa, Ilaria; Faretta, Mario; Diaspro, Alberto

    2005-08-01

    This report covers the two-photon activation and excitation properties of the PA-GFP, a photoactivatable variant of the Aequorea victoria green fluorescent protein in the spectral region from 720 to 920 nm. It is known from this special form of the molecule that it has an increased level of fluorescence emission when excited at 488 nm after irradiation at lambda approximately 413 nm, under single-photon excitation conditions. Here, we show that upon two-photon irradiation, PA-GFP yields activation in the spectral region from 720 to 840 nm. After photoactivation, the excitation spectrum shifts maintaining the very same emission spectrum of the single-photon case for the native and photoactivated protein. Additionally, when comparing the conventional photoactivation at lambda = 405 nm with a two-photon one, a sharper and better controllable three-dimensional volume of activation is obtained.

  16. PACAP Interacts with PAC1 Receptors to Induce Tissue Plasminogen Activator (tPA) Expression and Activity in Schwann Cell-Like Cultures

    PubMed Central

    Castorina, Alessandro; Waschek, James A.; Marzagalli, Rubina; Cardile, Venera; Drago, Filippo

    2015-01-01

    Regeneration of peripheral nerves depends on the abilities of rejuvenating axons to migrate at the injury site through cellular debris and altered extracellular matrix, and then grow along the residual distal nerve sheath conduit and reinnervate synaptic targets. Considerable evidence suggest that glial cells participate in this process, although the mechanisms remain to be clarified. In cell culture, regenerating neurites secrete PACAP, a peptide shown to induce the expression of the protease tissue plasminogen activator (tPA) in neural cell types. In the present studies, we tested the hypothesis that PACAP can stimulate peripheral glial cells to produce tPA. More specifically, we addressed whether or not PACAP promoted the expression and activity of tPA in the Schwann cell line RT4-D6P2T, which shares biochemical and physical properties with Schwann cells. We found that PACAP dose- and time-dependently stimulated tPA expression both at the mRNA and protein level. Such effect was mimicked by maxadilan, a potent PAC1 receptor agonist, but not by the PACAP-related homolog VIP, suggesting a PAC1-mediated function. These actions appeared to be mediated at least in part by the Akt/CREB signaling cascade because wortmannin, a PI3K inhibitor, prevented peptide-driven CREB phosphorylation and tPA increase. Interestingly, treatment with BDNF mimicked PACAP actions on tPA, but acted through both the Akt and MAPK signaling pathways, while causing a robust increase in PACAP and PAC1 expression. PACAP6-38 totally blocked PACAP-driven tPA expression and in part hampered BDNF-mediated effects. We conclude that PACAP, acting through PAC1 receptors, stimulates tPA expression and activity in a Akt/CREB-dependent manner to promote proteolytic activity in Schwann-cell like cultures. PMID:25658447

  17. Programs, Activities & Directions.

    ERIC Educational Resources Information Center

    National Inst. on Alcohol Abuse and Alcoholism (DHHS), Rockville, MD.

    In response to the magnitude of alcohol-related problems, the National Institute on Alcohol Abuse and Alcoholism (NIAAA) was established in 1971 with authority to conduct a comprehensive program of research and research training and to foster improved treatment and prevention programs for these problems. This publication gives a brief description…

  18. Murine monoclonal antibodies against murine uPA receptor produced in gene-deficient mice: inhibitory effects on receptor-mediated uPA activity in vitro and in vivo.

    PubMed

    Pass, Jesper; Jögi, Annika; Lund, Ida K; Rønø, Birgitte; Rasch, Morten G; Gårdsvoll, Henrik; Lund, Leif R; Ploug, Michael; Rømer, John; Danø, Keld; Høyer-Hansen, Gunilla

    2007-06-01

    Binding of urokinase plasminogen activator (uPA) to its cellular receptor, uPAR, potentiates plasminogen activation and localizes it to the cell surface. Focal plasminogen activation is involved in both normal and pathological tissue remodeling processes including cancer invasion. The interaction between uPA and uPAR therefore represents a potential target for anti-invasive cancer therapy. Inhibitors of the human uPA-uPAR interaction have no effect in the murine system. To enable in-vivo studies in murine cancer models we have now generated murine monoclonal antibodies (mAbs) against murine uPAR (muPAR) by immunizing uPAR-deficient mice with recombinant muPAR and screened for antibodies, which inhibit the muPA-muPAR interaction. Two of the twelve mAbs obtained, mR1 and mR2, interfered with the interaction between muPAR and the amino-terminal fragment of muPA (mATF) when analyzed by surface plasmon resonance. The epitope for mR1 is located on domain I of muPAR, while that of mR2 is on domains (II-III). In cell binding experiments using radiolabelled mATF, the maximal inhibition obtained with mR1 was 85% while that obtained with mR2 was 50%. The IC(50) value for mR1 was 0.67 nM compared to 0.14 nM for mATF. In an assay based on modified anthrax toxins, requiring cell-bound muPA activity for its cytotoxity, an approximately 50% rescue of the cells could be obtained by addition of mR1. Importantly, in-vivo efficacy of mR1 was demonstrated by the ability of mR1 to rescue mice treated with a lethal dose of uPA-activatable anthrax toxins.

  19. High-level expression of a novel recombinant human plasminogen activator (rhPA) in the milk of transgenic rabbits and its thrombolytic bioactivity in vitro.

    PubMed

    Song, Shaozheng; Ge, Xin; Cheng, Yaobin; Lu, Rui; Zhang, Ting; Yu, Baoli; Ji, Xueqiao; Qi, Zhengqiang; Rong, Yao; Yuan, Yuguo; Cheng, Yong

    2016-08-01

    The human tissue-type plasminogen activator (tPA) is a key kinase of fibrinolysis that plays an important role in dissolving fibrin clots to promote thrombolysis. The recombinant human plasminogen activator (rhPA) has more thrombolytic advantages than the wild type tPA. To increase the half-life and thrombolytic activity of tPA, a mutant containing only the essential K2 fibrin-binding and P activating plasminogen domains of the wild type tPA was cloned. This fragment was then inserted into goat β-casein regulatory sequences. Then, a mammary gland-specific expression vector, PCL25/rhPA, was constructed, and the transgenic rabbits were generated. In this study, 18 live transgenic founders (12♀, 6♂) were generated using pronuclear microinjection. Six transgenic rabbits were obtained, and the expression levels of rhPA in the milk had a range of 15.2-630 µg/ml. A fibrin agarose plate assay of rhPA showed that it had strong thrombolytic bioactivity in vitro, and the highest specific activity was >360 (360 times more than that of alteplase). The results indicated that the rhPA containing only the K2 and P domains is efficiently expressed with higher thrombolytic bioactivity in the milk of transgenic rabbits. Our study also demonstrated a new method for the large-scale production of clinically relevant recombinant pharmaceutical proteins in the mammary glands of transgenic rabbits.

  20. Multiple Activities Program: An Evaluation.

    ERIC Educational Resources Information Center

    Omaha Public Schools, NE.

    This is an evaluation report of the Multiple Activities Program, an ESEA Title I Program conducted in the Omaha Public Schools from September 1969 to September 1970. The report is designed as an adaptation of the C.I.P.P. evaluation model (context, input, process, and product). The needs, objectives, activities, and success of each strand within…

  1. Defining Standards and Policies for Promoting Physical Activity in Afterschool Programs

    ERIC Educational Resources Information Center

    Beets, Michael W.; Wallner, Megan; Beighle, Aaron

    2010-01-01

    Background: National guidelines exist that define "quality" afterschool programs (3-6 pm, ASP). No widely adopted national standards/policies exist, however, for ASP providers for the promotion of physical activity (PA). To address this gap, state-level ASP organizations have developed or adopted standards/policies related to PA. The extent to…

  2. Static or dynamic predictors of physical activity (PA)? A tracking study based on 12- and 38-month follow-ups in older adults.

    PubMed

    de Souto Barreto, Philipe; Ferrandez, Anne-Marie

    2014-01-01

    Studies that investigated stability of PA in older populations are scarce. Moreover, no studies used dynamic indicators to predict PA trajectories. The purpose of the present study were to investigate PA stability overtime, and to examine if changes in self-reported physical function (dynamic indicator) are better predictors of trajectories of PA than baseline measures of physical function (static indicator). This is a prospective postal survey with two time-point follow-ups: 12 and 38 months. Participants were older adults aged ≥ 60 years, and members of the medical insurance scheme of the French national education system. They responded to a self-report questionnaire on PA and general health status at three different times: baseline, 12- and 38-month follow-ups (n=243 for the 12-month follow-up; n=164 for the 38-month follow-up). Overtime analyses of PA showed a moderate-to-good stability with regard to both duration and volume of PA; however, a decrease in stability for vigorous PA was found between 12- and 38-month follow-ups. Both baseline measure and changes in physical function predicted PA trajectories, but magnitudes of associations were stronger for the dynamic indicator. Moreover, change in physical function was the only predictor of both becoming active compared with Inactive (reduced probability) and becoming inactive compared to Active (increased probability). In conclusion, a dynamic indicator of physical function is a better predictor of PA variation than static indicators.

  3. Staff's perceptions of the use of evidence-based physical activity promotion strategies for promoting girls' physical activity at afterschool programs: a qualitative study.

    PubMed

    Dinkel, Danae; Huberty, Jennifer; Beets, Michael; Tibbits, Melissa

    2014-08-01

    There is a need to improve girls' physical activity (PA) in afterschool programs as girls' PA levels are consistently lower than boys'. An evidence-based professional development framework, the 5 Ms, has been effective in helping staff to improve PA in both girls and boys but further improvements in girls' PA are needed. Little is known about staff's perceptions of using PA promotion strategies to promote girls' PA. Therefore, the purpose of this study was to explore staff perceptions of the use of evidence-based PA promotion strategies for promoting PA in girls. Semi-structured interviews were conducted with staff from three community-based afterschool programs located within a school setting (n=18). Data were analyzed using the process of immersion/crystallization. A majority of staff had some knowledge of PA promotion strategies but few staff consistently utilized these strategies and a majority felt several strategies were unnecessary (i.e., having a PA policy). Newer staff reported depending on senior staff to promote PA in girls. Overall, findings suggest that staff's perceptions may impact their use of PA promotions strategies. The results of this study will contribute to the enhancement of an existing staff training framework (the 5 Ms) to improve girls' PA in afterschool programs.

  4. Evaluation of GoGirlGo!; A practitioner based program to improve physical activity

    PubMed Central

    2014-01-01

    Background GoGirlGo! (GGG) is designed to increase girls’ physical activity (PA) using a health behavior and PA-based curriculum and is widely available for free to afterschool programs across the nation. However, GGG has not been formally evaluated. The purpose of this pilot study was to evaluate the effectiveness of the GGG curricula to improve PA, and self-efficacy for and enjoyment of PA in elementary aged girls (i.e., 5-13 years). Methods Nine afterschool programs were recruited to participate in the pilot (within subjects repeated measures design). GGG is a 12-week program, with a once a week, one-hour lesson with 30 minutes of education and 30 minutes of PA). Data collection occurred at baseline, mid (twice), post, and at follow-up (3-months after the intervention ended). PA was assessed via accelerometry at each time point. Self-efficacy for and enjoyment of PA was measured using the Self-Efficacy Scale and the Short-PA enjoyment scale and was assessed at baseline, post, and follow-up. Fidelity was assessed at midpoint. Results Across all age groups there was a statistically significant increase in PA. Overall, on days GGG was offered girls accumulated an average of 11 minutes of moderate-to-vigorous PA compared to 8 minutes during non-GGG days. There was a statistically significant difference in girls’ self-efficacy for PA reported between baseline and post, which was maintained at follow-up. An improvement in enjoyment of PA for girls was found between baseline and follow-up. According to fidelity assessment, 89% of the activities within the curriculum were completed each lesson. Girls appeared to respond well to the curriculum but girls 5-7 years had difficulties paying attention and understanding discussion questions. Conclusions Even though there were statistically significant differences in self-efficacy for PA and enjoyment of PA, minimal increases in girls’ PA were observed. GGG curricula improvements are warranted. Future GGG programming

  5. Preclinical testing of the nitroimidazopyran PA-824 for activity against Mycobacterium tuberculosis in a series of in vitro and in vivo models.

    PubMed

    Lenaerts, Anne J; Gruppo, Veronica; Marietta, Karen S; Johnson, Christine M; Driscoll, Diane K; Tompkins, Nicholas M; Rose, Jerry D; Reynolds, Robert C; Orme, Ian M

    2005-06-01

    This study extends earlier reports regarding the in vitro and in vivo efficacies of the nitroimidazopyran PA-824 against Mycobacterium tuberculosis. PA-824 was tested in vitro against a broad panel of multidrug-resistant clinical isolates and was found to be highly active against all isolates (MIC<1 microg/ml). The activity of PA-824 against M. tuberculosis was also assessed grown under conditions of oxygen depletion. PA-824 showed significant activity at 2, 10, and 50 microg/ml, similar to that of metronidazole, in a dose-dependent manner. In a short-course mouse infection model, the efficacy of PA-824 at 50, 100, and 300 mg/kg of body weight formulated in methylcellulose or cyclodextrin/lecithin after nine oral treatments was compared with those of isoniazid, rifampin, and moxifloxacin. PA-824 at 100 mg/kg in cyclodextrin/lecithin was as active as moxifloxacin at 100 mg/kg and isoniazid at 25 mg/kg and was slightly more active than rifampin at 20 mg/kg. Long-term treatment with PA-824 at 100 mg/kg in cyclodextrin/lecithin reduced the bacterial load below 500 CFU in the lungs and spleen. No significant differences in activity between PA-824 and the other single drug treatments tested (isoniazid at 25 mg/kg, rifampin at 10 mg/kg, gatifloxacin at 100 mg/kg, and moxifloxacin at 100 mg/kg) could be observed. In summary, its good activity in in vivo models, as well as its activity against multidrug-resistant M. tuberculosis and against M. tuberculosis isolates in a potentially latent state, makes PA-824 an attractive drug candidate for the therapy of tuberculosis. These data indicate that there is significant potential for effective oral delivery of PA-824 for the treatment of tuberculosis.

  6. Increasing tPA Activity in Astrocytes Induced by Multipotent Mesenchymal Stromal Cells Facilitate Neurite Outgrowth after Stroke in the Mouse

    PubMed Central

    Xin, Hongqi; Li, Yi; Shen, Li Hong; Liu, Xianshuang; Wang, Xinli; Zhang, Jing; Pourabdollah-Nejad D, Siamak; Zhang, Chunling; Zhang, Li; Jiang, Hao; Zhang, Zheng Gang; Chopp, Michael

    2010-01-01

    We demonstrate that tissue plasminogen activator (tPA) and its inhibitors contribute to neurite outgrowth in the central nervous system (CNS) after treatment of stroke with multipotent mesenchymal stromal cells (MSCs). In vivo, administration of MSCs to mice subjected to middle cerebral artery occlusion (MCAo) significantly increased activation of tPA and downregulated PAI-1 levels in the ischemic boundary zone (IBZ) compared with control PBS treated mice, concurrently with increases of myelinated axons and synaptophysin. In vitro, MSCs significantly increased tPA levels and concomitantly reduced plasminogen activator inhibitor 1 (PAI-1) expression in astrocytes under normal and oxygen and glucose deprivation (OGD) conditions. ELISA analysis of conditioned medium revealed that MSCs stimulated astrocytes to secrete tPA. When primary cortical neurons were cultured in the conditioned medium from MSC co-cultured astrocytes, these neurons exhibited a significant increase in neurite outgrowth compared to conditioned medium from astrocytes alone. Blockage of tPA with a neutralizing antibody or knock-down of tPA with siRNA significantly attenuated the effect of the conditioned medium on neurite outgrowth. Addition of recombinant human tPA into cortical neuronal cultures also substantially enhanced neurite outgrowth. Collectively, these in vivo and in vitro data suggest that the MSC mediated increased activation of tPA in astrocytes promotes neurite outgrowth after stroke. PMID:20140248

  7. Protease-Activated Receptor 2 (PAR2) Is Upregulated by Acanthamoeba Plasminogen Activator (aPA) and Induces Proinflammatory Cytokine in Human Corneal Epithelial Cells

    PubMed Central

    Tripathi, Trivendra; Abdi, Mahshid; Alizadeh, Hassan

    2014-01-01

    Purpose. Acanthamoeba plasminogen activator (aPA) is a serine protease elaborated by Acanthamoeba trophozoites that facilitates the invasion of trophozoites to the host and contributes to the pathogenesis of Acanthamoeba keratitis (AK). The aim of this study was to explore if aPA stimulates proinflammatory cytokine in human corneal epithelial (HCE) cells via the protease-activated receptors (PARs) pathway. Methods. Acanthamoeba castellanii trophozoites were grown in peptone-yeast extract glucose for 7 days, and the supernatants were collected and centrifuged. The aPA was purified using the fast protein liquid chromatography system, and aPA activity was determined by zymography assays. Human corneal epithelial cells were incubated with or without aPA (100 μg/mL), PAR1 agonists (thrombin, 10 μM; TRAP-6, 10 μM), and PAR2 agonists (SLIGRL-NH2, 100 μM; AC 55541, 10 μM) for 24 and 48 hours. Inhibition of PAR1 and PAR2 involved preincubating the HCE cells for 1 hour with the antagonist of PAR1 (SCH 79797, 60 μM) and PAR2 (FSLLRY-NH2, 100 μM) with or without aPA. Human corneal epithelial cells also were preincubated with PAR1 and PAR2 antagonists and then incubated with or without PAR1 agonists (thrombin and TRAP-6) and PAR2 agonists (SLIGRL-NH2 and AC 55541). Expression of PAR1 and PAR2 was examined by quantitative RT-PCR (qRT-PCR), flow cytometry, and immunocytochemistry. Interleukin-8 expression was quantified by qRT-PCR and ELISA. Results. Human corneal epithelial cells constitutively expressed PAR1 and PAR2 mRNA. Acanthamoeba plasminogen activator and PAR2 agonists significantly upregulated PAR2 mRNA expression (1- and 2-fold, respectively) (P < 0.05). Protease-activated receptor 2 antagonist significantly inhibited aPA, and PAR2 agonists induced PAR2 mRNA expression in HCE cells (P < 0.05). Protease-activated receptor 1 agonists, but not aPA, significantly upregulated PAR1 mRNA expression, which was significantly inhibited by PAR1 antagonist in HCE cells

  8. Pyrrolnitrin and Hydrogen Cyanide Production by Pseudomonas chlororaphis Strain PA23 Exhibits Nematicidal and Repellent Activity against Caenorhabditis elegans

    PubMed Central

    Nandi, Munmun; Selin, Carrie; Brassinga, Ann Karen C.; Belmonte, Mark F.; Fernando, W. G. Dilantha; Loewen, Peter C.; de Kievit, Teresa R.

    2015-01-01

    Pseudomonas chlororaphis strain PA23 is a biocontrol agent able to suppress growth of the fungal pathogen Sclerotinia sclerotiorum. This bacterium produces an arsenal of exometabolites including pyrrolnitrin (PRN), phenazine (PHZ), hydrogen cyanide (HCN), and degradative enzymes. Production of these compounds is controlled at both the transcriptional and posttranscriptional levels by the Gac-Rsm system, RpoS, PsrA, and the Phz quorum-sensing system. Beyond pathogen-suppression, the success of a biocontrol agent is dependent upon its ability to establish itself in the environment where predation by bacterivorous organisms, including nematodes, may threaten persistence. The focus of this study was to investigate whether PA23 is able to resist grazing by Caenorhabditis elegans and to define the role played by exoproducts in the bacterial-nematode interaction. We discovered that both PRN and HCN contribute to fast- and slow-killing of C. elegans. HCN is well-established as having lethal effects on C. elegans; however, PRN has not been reported to be nematicidal. Exposure of L4 stage nematodes to purified PRN reduced nematode viability in a dose-dependent fashion and led to reduced hatching of eggs laid by gravid adults. Because bacterial metabolites can act as chemoattractants or repellents, we analyzed whether PA23 exhibited attractant or repulsive properties towards C. elegans. Both PRN and HCN were found to be potent repellents. Next we investigated whether the presence of C. elegans would elicit changes in PA23 gene activity. Co-culturing the two organisms increased expression of a number of genes associated with biocontrol, including phzA, hcnA, phzR, phzI, rpoS and gacS. Exoproduct analysis showed that PHZ and autoinducer signals were upregulated, consistent with the gene expression profiles. Collectively, these findings indicate that PA23 is able to sense the presence of C. elegans and it is able to both repel and kill the nematodes, which should facilitate

  9. Control of active nitrogen species used for PA-MBE growth of group III nitrides on Si

    NASA Astrophysics Data System (ADS)

    Ohachi, Tadashi; Yamabe, Nobuhiko; Yamamoto, Yuka; Wada, Motoi; Ariyada, Osamu

    2011-03-01

    A new spiral parallel mesh electrode (PME) is presented to control active nitrogen species in plasma-assisted molecular beam epitaxial (PA-MBE) growth of group III nitrides and their alloys. Direct flux of active nitrogen from radio frequency inductive coupled plasma (rf-ICP) discharge was able to be measured using a mesh electrode for filtering charge particles and electron emission due to the self-ionization of nitrogen atoms on a negatively biased electrode. In situ measurement of direct nitrogen atom fluxes using the spiral PME during PA-MBE growth of GaN and AlN on Si substrates is investigated. A linear rf power dependence of direct flux of active species on atoms such as nitrogen (N+N*), where N and N* were ground and excited atoms, respectively, from a rf-ICP was confirmed by the spiral PME. An indirect flux of nitrogen adsorbed (ADS) atoms (N+N*) during discharge was also monitored by the spiral PME and received influence of the wall surface of the growth chamber. ADS nitrogen atoms are able to be used for nitridation of Si surface to grow a double buffer layer (DBL) AlN/β-Si3N4/Si.

  10. Environmental health program activities

    NASA Technical Reports Server (NTRS)

    Bergtholdt, C. P.

    1969-01-01

    Activities reported include studies on toxic air contaminants, excessive noise, poor lighting, food sanitation, water pollution, and exposure to nonionizing radiation as health hazards. Formulations for a radiological health manual provide guidance to personnel in the procurement and safe handling of radiation producing equipment and Apollo mission planning. A literature search and development of a water analysis laboratory are outlined to obtain information regarding microbiological problems involving potable water, waste management, and personal hygiene.

  11. Critical Role of the PA-X C-Terminal Domain of Influenza A Virus in Its Subcellular Localization and Shutoff Activity

    PubMed Central

    Hayashi, Tsuyoshi; Chaimayo, Chutikarn; McGuinness, James

    2016-01-01

    ABSTRACT PA-X is a recently identified influenza virus protein that is composed of the PA N-terminal 191 amino acids and unique C-terminal 41 or 61 residues. We and others showed that PA-X has a strong ability to suppress host protein synthesis via host mRNA decay, which is mediated by endonuclease activity in its N-terminal domain (B. W. Jagger, H. M. Wise, J. C. Kash, K. A. Walters, N. M. Wills, Y. L. Xiao, R. L. Dunfee, L. M. Schwartzman, A. Ozinsky, G. L. Bell, R. M. Dalton, A. Lo, S. Efstathiou, J. F. Atkins, A. E. Firth, J. K. Taubenberger, and P. Digard, 2012, Science 337:199–204, http://dx.doi.org/10.1126/science.1222213, and E. A. Desmet, K. A. Bussey, R. Stone, and T. Takimoto, 2013, J Virol 87:3108–3118, http://dx.doi.org/10.1128/JVI.02826-12). However, the mechanism of host mRNA degradation, especially where and how PA-X targets mRNAs, has not been analyzed. In this study, we determined the localization of PA-X and the role of the C-terminal unique region in shutoff activity. Quantitative subcellular localization analysis revealed that PA-X was located equally in both cytoplasm and nucleus. By characterizing a series of PA-X C-terminal deletion mutants, we found that the first 9 amino acids were sufficient for nuclear localization, but an additional 6 residues were required to induce the maximum shutoff activity observed with intact PA-X. Importantly, forced nuclear localization of the PA-X C-terminal deletion mutant enhanced shutoff activity, highlighting the ability of nuclear PA-X to degrade host mRNAs more efficiently. However, PA-X also inhibited luciferase expression from transfected mRNAs synthesized in vitro, suggesting that PA-X also degrades mRNAs in the cytoplasm. Among the basic amino acids in the PA-X C-terminal region, 3 residues, 195K, 198K, and 199R, were identified as key residues for inducing host shutoff and nuclear localization. Overall, our data indicate a critical role for the 15 residues in the PA-X C-terminal domain in

  12. Antiviral Activity of Bay 41-4109 on Hepatitis B Virus in Humanized Alb-uPA/SCID Mice

    PubMed Central

    Brezillon, Nicolas; Brunelle, Marie-Noëlle; Massinet, Hélène; Giang, Eric; Lamant, Céline; DaSilva, Lucie; Berissi, Sophie; Belghiti, Jacques; Hannoun, Laurent; Puerstinger, Gherard; Wimmer, Eva; Neyts, Johan; Hantz, Olivier; Soussan, Patrick; Morosan, Serban; Kremsdorf, Dina

    2011-01-01

    Current treatments for HBV chronic carriers using interferon alpha or nucleoside analogues are not effective in all patients and may induce the emergence of HBV resistant strains. Bay 41-4109, a member of the heteroaryldihydropyrimidine family, inhibits HBV replication by destabilizing capsid assembly. The aim of this study was to determine the antiviral effect of Bay 41-4109 in a mouse model with humanized liver and the spread of active HBV. Antiviral assays of Bay 41-4109 on HepG2.2.15 cells constitutively expressing HBV, displayed an IC50 of about 202 nM with no cell toxicity. Alb-uPA/SCID mice were transplanted with human hepatocytes and infected with HBV. Ten days post-infection, the mice were treated with Bay 41-4109 for five days. During the 30 days of follow-up, the HBV load was evaluated by quantitative PCR. At the end of treatment, decreased HBV viremia of about 1 log(10) copies/ml was observed. By contrast, increased HBV viremia of about 0.5 log(10) copies/ml was measured in the control group. Five days after the end of treatment, a rebound of HBV viremia occurred in the treated group. Furthermore, 15 days after treatment discontinuation, a similar expression of the viral capsid was evidenced in liver biopsies. Our findings demonstrate that Bay 41-4109 displayed antiviral properties against HBV in humanized Alb-uPA/SCID mice and confirm the usefulness of Alb-uPA/SCID mice for the evaluation of pharmaceutical compounds. The administration of Bay 41-4109 may constitute a new strategy for the treatment of patients in escape from standard antiviral therapy. PMID:22162746

  13. Active Sites Environmental Monitoring Program: Program plan

    SciTech Connect

    Ashwood, T.L.; Wickliff, D.S.; Morrissey, C.M.

    1992-02-01

    The Active Sites Environmental Monitoring Program (ASEMP), initiated in 1989, provides early detection and performance monitoring of transuranic (TRU) waste and active low-level waste (LLW) facilities at Oak Ridge National Laboratory (ORNL) in accordance with US Department of Energy (DOE) Order 5820.2A. Active LLW facilities in Solid Waste Storage Area (SWSA) 6 include Tumulus I and Tumulus II, the Interim Waste Management Facility (IWMF), LLW silos, high-range wells, asbestos silos, and fissile wells. The tumulus pads and IWMF are aboveground, high-strength concrete pads on which concrete vaults containing metal boxes of LLW are placed; the void space between the boxes and vaults is filled with grout. Eventually, these pads and vaults will be covered by an engineered multilayered cap. All other LLW facilities in SWSA 6 are below ground. In addition, this plan includes monitoring of the Hillcut Disposal Test Facility (HDTF) in SWSA 6, even though this facility was completed prior to the data of the DOE order. In SWSA 5 North, the TRU facilities include below-grade engineered caves, high-range wells, and unlined trenches. All samples from SWSA 6 are screened for alpha and beta activity, counted for gamma-emitting isotopes, and analyzed for tritium. In addition to these analytes, samples from SWSA 5 North are analyzed for specific transuranic elements.

  14. Physical and Social-Motivational Contextual Correlates of Youth Physical Activity in Underresourced Afterschool Programs.

    PubMed

    Zarrett, Nicole; Sorensen, Carl; Cook, Brittany Skiles

    2015-08-01

    Afterschool programs (ASPs) have become increasingly recognized as a key context to support youth daily physical activity (PA) accrual. The purpose of the present study was to assess the physical and social-motivational climate characteristics of ASPs associated with youth PA, and variations in contextual correlates of PA by youth sex. Systematic observations of 7 ASPs serving underserved youth (minority, low income) was conducted using the System for Observing Play and Leisure Activity in Youth and a social-motivational climate observation tool founded on self-determination theory. For five program days at each site, teams of two coders conducted continuous observations of youth PA (sedentary, moderate, vigorous), five physical features (e.g., equipment availability), eight staff interactions (e.g., encourage PA), and seven motivational climate components (e.g., inclusive). Aligned with previous research, regressions controlling for variations by site indicated that organized PA, provision of portable equipment, and staff PA participation and supervision are key correlates of youth PA. Moreover, as the first study to systematically observe motivational-context characteristics of ASPs, we identified several key modifiable motivational features that are necessary to address in order to increase youth engagement in PA during the out-of-school hours. Among motivational features assessed, "relatedness" components (positive peer relations, inclusive/cooperative activities) were primary correlates of girls' PA. In contrast, all three motivational features specified by self-determination theory (support for autonomy, mastery/competence, and inclusion/relatedness) were correlated with boys' PA. Findings are discussed in terms of policy and practice for understanding strengths and needs of ASPs to effectively engage youth in PA.

  15. PA residues in the 2009 H1N1 pandemic influenza virus enhance avian influenza virus polymerase activity in mammalian cells.

    PubMed

    Bussey, Kendra A; Desmet, Emily A; Mattiacio, Jonelle L; Hamilton, Alice; Bradel-Tretheway, Birgit; Bussey, Howard E; Kim, Baek; Dewhurst, Stephen; Takimoto, Toru

    2011-07-01

    The 2009 pandemic influenza virus (pH1N1) is a swine-origin reassortant containing human, avian, and swine influenza genes. We have previously shown that the polymerase complex of the pH1N1 strain A/California/04/2009 (Cal) is highly active in mammalian 293T cells, despite the avian origin of both its PA and PB2. In this study, we analyzed the polymerase residues that are responsible for high pH1N1 polymerase activity in the mammalian host. Characterization of polymerase complexes containing various combinations of Cal and avian influenza virus A/chicken/Nanchang/3-120/01 (H3N2) (Nan) by reporter gene assay indicates that Cal PA, but not PB2, is a major contributing factor to high Cal polymerase activity in 293T cells. In particular, Cal PA significantly activates the otherwise inactive Nan polymerase at 37 and 39°C but not at the lower temperature of 34°C. Further analysis using site-directed mutagenesis showed that the Cal PA residues 85I, 186S, and 336M contribute to enhanced activity of the Cal polymerase. Recombinant A/WSN/33 (H1N1) (WSN) viruses containing Nan NP and polymerase (PA, PB1, PB2) genes with individual mutations in PA at residues 85, 186, and 336 produced higher levels of viral protein than the virus containing wild-type (WT) Nan PA. Interestingly, compared to the WT, the virus containing the 85I mutation grew faster in human A549 cells and the 336M mutation most significantly enhanced pathogenicity in a mouse model, among the three PA mutations tested. Our results suggest that multiple mutations in PA, which were rarely present in previous influenza isolates, are involved in mammalian adaptation and pathogenicity of the 2009 pH1N1.

  16. Formation of Raman Scattering Wings around H alpha, H beta, and Pa alpha in Active Galactic Nuclei

    NASA Astrophysics Data System (ADS)

    Chang, Seok-Jun; Heo, Jeong-Eun; Di Mille, Francesco; Angeloni, Rodolfo; Palma, Tali; Lee, Hee-Won

    2015-12-01

    Powered by a supermassive black hole with an accretion disk, the spectra of active galactic nuclei (AGNs) are characterized by prominent emission lines including Balmer lines. The unification schemes of AGNs require the existence of a thick molecular torus that may hide the broad emission line region from the view of observers near the equatorial direction. In this configuration, one may expect that the far-UV radiation from the central engine can be Raman scattered by neutral hydrogen to reappear around Balmer and Paschen emission lines, which can be identified with broad wings. We produce Hα, Hβ, and Paα wings using a Monte Carlo technique to investigate their properties. The neutral scattering region is assumed to be a cylindrical torus specified by the inner and outer radii and the height. While the covering factor of the scattering region affects the overall strengths of the wings, the wing widths are primarily dependent on the neutral hydrogen column density {N}{{H} {{I}}} being roughly proportional to {N}{{H} {{I}}}1/2. In particular, with {N}{{H} {{I}}}={10}23 {{cm}}-2 the Hα wings typically show a width ∼ 2× {10}4 {km} {{{s}}}-1. We also find that Hα and Paα wing profiles are asymmetric with the red part stronger than the blue part and an opposite behavior is seen for Hβ wings.

  17. Assessing sustainability of Lifestyle Education for Activity Program (LEAP).

    PubMed

    Saunders, R P; Pate, R R; Dowda, M; Ward, D S; Epping, J N; Dishman, R K

    2012-04-01

    Sustained intervention effects are needed for positive health impacts in populations; however, few published examples illustrate methods for assessing sustainability in health promotion programs. This paper describes the methods for assessing sustainability of the Lifestyle Education for Activity Program (LEAP). LEAP was a comprehensive school-based intervention that targeted change in instructional practices and the school environment to promote physical activity (PA) in high school girls. Previous reports indicated that significantly more girls in the intervention compared with control schools reported engaging in vigorous PA, and positive long-term effects on vigorous PA also were observed for girls in schools that most fully implemented and maintained the intervention 3 years following the active intervention. In this paper, the seven steps used to assess sustainability in LEAP are presented; these steps provide a model for assessing sustainability in health promotion programs in other settings. Unique features of the LEAP sustainability model include assessing sustainability of changes in instructional practices and the environment, basing assessment on an essential element framework that defined complete and acceptable delivery at the beginning of the project, using multiple data sources to assess sustainability, and assessing implementation longitudinally.

  18. Assessing sustainability of Lifestyle Education for Activity Program (LEAP)

    PubMed Central

    Saunders, R. P.; Pate, R. R.; Dowda, M.; Ward, D. S.; Epping, J. N.; Dishman, R. K.

    2012-01-01

    Sustained intervention effects are needed for positive health impacts in populations; however, few published examples illustrate methods for assessing sustainability in health promotion programs. This paper describes the methods for assessing sustainability of the Lifestyle Education for Activity Program (LEAP). LEAP was a comprehensive school-based intervention that targeted change in instructional practices and the school environment to promote physical activity (PA) in high school girls. Previous reports indicated that significantly more girls in the intervention compared with control schools reported engaging in vigorous PA, and positive long-term effects on vigorous PA also were observed for girls in schools that most fully implemented and maintained the intervention 3 years following the active intervention. In this paper, the seven steps used to assess sustainability in LEAP are presented; these steps provide a model for assessing sustainability in health promotion programs in other settings. Unique features of the LEAP sustainability model include assessing sustainability of changes in instructional practices and the environment, basing assessment on an essential element framework that defined complete and acceptable delivery at the beginning of the project, using multiple data sources to assess sustainability, and assessing implementation longitudinally. PMID:22156233

  19. Determining the Reach of a Home-Based Physical Activity Program for Older Adults within the Context of a Randomized Controlled Trial

    ERIC Educational Resources Information Center

    Harden, Samantha M.; Fanning, Jason T.; Motl, Robert W.; McAuley, Edward; Estabrooks, Paul A.

    2014-01-01

    Determining the reach of physical activity (PA) programs is challenging due to inconsistent reporting across studies. The purpose of this study was to document multiple indicators of program reach for a 6-month, Digital Versatile Disc (DVD)-delivered home-based PA program. Radio, newspaper and direct mailing advertisements were tracked to…

  20. Urinary-type plasminogen activator (uPA) and its receptor (uPAR) in squamous cell carcinoma of the oral cavity.

    PubMed

    Shi, Zonggao; Stack, M Sharon

    2007-10-15

    OSCC (oral squamous cell carcinoma) is the most common oral malignancy and is estimated to affect approx. 350000 new patients worldwide this year. OSCC is characterized by a high degree of morbidity and mortality, as most patients exhibit local, regional and distant metastasis at the time of diagnosis. Recent genome-wide screening efforts have identified the serine proteinase uPA (urinary-type plasminogen activator, also known as urokinase) as a strong biomarker for prediction of poor disease outcome and a key candidate for molecular classification of oral neoplasms using a 'gene signature' approach. The proteinase uPA binds a surface-anchored receptor designated uPAR (uPA receptor), focalizing proteolytic activity to the pericellular milieu. Furthermore, uPA-uPAR can interact with transmembrane proteins to modify multiple signal transduction pathways and influence a wide variety of cellular behaviours. Correlative clinical data show elevated uPA-uPAR in oral tumour tissues, with tumours exhibiting high levels of both uPA and uPAR as the most invasive. Combined in vitro, pre-clinical and clinical data support the need for further analysis of uPA-uPAR as a prognostic indicator as well as a potential therapeutic target in OSCC.

  1. GSK-3β inhibitor TWS119 attenuates rtPA-induced hemorrhagic transformation and activates the Wnt/β-catenin signaling pathway after acute ischemic stroke in rats

    PubMed Central

    Wang, Wei; Li, Mingchang; Wang, Yuefei; Li, Qian; Deng, Gang; Wan, Jieru; Yang, Qingwu

    2016-01-01

    Hemorrhagic transformation (HT) is a devastating complication for patients with acute ischemic stroke who are treated with tissue plasminogen activator (tPA). It is associated with high morbidity and mortality, but no effective treatments are currently available to reduce HT risk. Therefore, methods to prevent HT are urgently needed. In this study, we used TWS119, an inhibitor of glycogen synthase kinase 3β (GSK-3β), to evaluate the role of the Wnt/β-catenin signaling pathway in recombinant tPA (rtPA)-induced HT. Sprague–Dawley rats were subjected to a middle cerebral artery occlusion (MCAO) model of ischemic stroke and then were administered rtPA, rtPA combined with TWS119, or vehicle at 4 h. The animals were sacrificed 24 h after infarct induction. Rats treated with rtPA showed evident HT, had more severe neurologic deficit, brain edema, and blood–brain barrier breakdown, and had larger infarction volume than did the vehicle group. Rats treated with TWS119 had significantly improved outcomes compared with those of rats treated with rtPA alone. In addition, Western blot analysis showed that TWS119 increased the protein expression of β-catenin, claudin-3, and ZO-1 while suppressing the expression of GSK-3β. These results suggest that TWS119 reduces rtPA-induced HT and attenuates blood–brain barrier disruption, possibly through activation of the Wnt/β-catenin signaling pathway. This study provides a potential therapeutic strategy to prevent tPA-induced HT after acute ischemic stroke. PMID:26671619

  2. Clean Coal Program Research Activities

    SciTech Connect

    Larry Baxter; Eric Eddings; Thomas Fletcher; Kerry Kelly; JoAnn Lighty; Ronald Pugmire; Adel Sarofim; Geoffrey Silcox; Phillip Smith; Jeremy Thornock; Jost Wendt; Kevin Whitty

    2009-03-31

    Although remarkable progress has been made in developing technologies for the clean and efficient utilization of coal, the biggest challenge in the utilization of coal is still the protection of the environment. Specifically, electric utilities face increasingly stringent restriction on the emissions of NO{sub x} and SO{sub x}, new mercury emission standards, and mounting pressure for the mitigation of CO{sub 2} emissions, an environmental challenge that is greater than any they have previously faced. The Utah Clean Coal Program addressed issues related to innovations for existing power plants including retrofit technologies for carbon capture and sequestration (CCS) or green field plants with CCS. The Program focused on the following areas: simulation, mercury control, oxycoal combustion, gasification, sequestration, chemical looping combustion, materials investigations and student research experiences. The goal of this program was to begin to integrate the experimental and simulation activities and to partner with NETL researchers to integrate the Program's results with those at NETL, using simulation as the vehicle for integration and innovation. The investigators also committed to training students in coal utilization technology tuned to the environmental constraints that we face in the future; to this end the Program supported approximately 12 graduate students toward the completion of their graduate degree in addition to numerous undergraduate students. With the increased importance of coal for energy independence, training of graduate and undergraduate students in the development of new technologies is critical.

  3. Students' Daily Physical Activity Behaviors: The Role of Quality Physical Education in a Comprehensive School Physical Activity Program

    ERIC Educational Resources Information Center

    Chen, Wiyun; Hypnar, Andrew J.; Mason, Steve A.; Zalmout, Sandy

    2014-01-01

    The purpose of this study was to examine the contribution of quality physical education (QPET) in a Comprehensive School Physical Activity Program (CSPAP) which is intended to promote physical activity (PA) behaviors in and outside of schools. Participants were nine elementary physical education teachers and their fourth- and fifth-grade students…

  4. Production of a urokinase plasminogen activator-IgG fusion protein (uPA-IgG) in the baculovirus expression system.

    PubMed

    Kost, T A; Ignar, D M; Clay, W C; Andrews, J; Leray, J D; Overton, L; Hoffman, C R; Kilpatrick, K E; Ellis, B; Emerson, D L

    1997-04-29

    Numerous studies have demonstrated the importance of urokinase plasminogen activator (uPA) and its receptor, uPAR, in the processes of tumor progression and metastasis. Thus, the uPA/uPAR interaction may represent an important target for inhibiting metastatic disease. The baculovirus expression system was used to produce high levels of a secreted uPA-Immunoglobulin G fusion protein (uPA-IgG) which could then be used for displacing uPA from the surface of tumor cells. The recombinant uPA-IgG fusion protein was placed under the control of either the viral polyhedrin promoter or a copy of the viral basic protein promoter. Recombinant viruses were then used to infect Sf9 and BTI-Tn-5B1-4 cells. Infection of both cell types resulted in the production of secreted uPA-IgG. The molecular mass of the secreted protein as determined by SDS-PAGE was approximately 40 kDa. The highest level of secreted uPA-IgG, 444 microg/ml, was found in the culture medium of BTI-Tn-5B1-4 cells 72 h post-infection with the basic protein promoter-uPA-IgG virus. In the case of Sf9 cells, the highest level of secreted protein was 195 microg/ml. The amount of cell-associated uPA-IgG in infected BTI-Tn-5B1-4 cells was significantly less than that of infected Sf9 cells, reflecting the superior secretory capability of the BTI-Tn-5B1-4 cells. The uPA-IgG was readily purified using a combination of zinc chelate and sephacryl S-100 column chromatography. Routinely, greater than 100 mg of greater than 95% pure protein could be obtained per liter of culture medium collected at 72 h post-infection of BTI-Tn-5B1-4 cells with the basic protein promoter virus. BIAcore analysis and competition binding assays using LOX human malignant melanoma cells expressing uPAR indicated that the purified recombinant protein possessed similar ligand binding characteristics to that of human uPA.

  5. TGF-β2 promotes RPE cell invasion into a collagen gel by mediating urokinase-type plasminogen activator (uPA) expression.

    PubMed

    Sugioka, Koji; Kodama, Aya; Okada, Kiyotaka; Iwata, Mihoko; Yoshida, Koji; Kusaka, Shunji; Matsumoto, Chota; Kaji, Hiroshi; Shimomura, Yoshikazu

    2013-10-01

    Transforming growth factor-beta (TGF-β) is one of the main epithelial-mesenchymal transition (EMT)-inducing factors. In general, TGF-β-induced EMT promotes cell migration and invasion. TGF-β also acts as a potent regulator of pericellular proteolysis by regulating the expression and secretion of plasminogen activators. Urokinase-type plasminogen activator (uPA) is a serine protease that binds to its cell surface receptor (uPAR) with high affinity. uPA binding to uPAR stimulates uPAR's interaction with transmembrane proteins, such as integrins, to regulate cytoskeletal reorganization and cell migration, differentiation and proliferation. However, the influence of TGF-β and the uPA/uPAR system on EMT in retinal pigment epithelial (RPE) cells is still unclear. The purpose of this study was to determine the effect of TGF-β2, which is the predominant isoform in the retina, and the uPA/uPAR system on RPE cells. In this study, we first examined the effect of TGF-β2 and/or the inhibitor of uPA (u-PA-STOP(®)) on the proliferation of a human retinal pigment epithelial cell line (ARPE-19 cells). Treatment with TGF-β2 or u-PA-STOP(®) suppressed cell proliferation. Combination treatment of TGF-β2 and u-PA-STOP(®) enhanced cell growth suppression. Furthermore, western blot analysis, fibrin zymography and real-time reverse transcription PCR showed that that TGF-β2 induced EMT in ARPE-19 cells and that the expression of uPA and uPAR expression was up-regulated during EMT. The TGF-β inhibitor SB431542 suppressed TGF-β2-stimulated uPA expression and secretion but did not suppress uPAR expression. Furthermore, we seeded ARPE-19 cells onto Transwell chambers and allowed them to invade the collagen matrix in the presence of TGF-β2 alone or with TGF-β2 and u-PA-STOP(®). TGF-β2 treatment induced ARPE-19 cell invasion into the collagen gel. Treatment with a combination of TGF-β2 and the uPA inhibitor strongly inhibited ARPE-19 cell invasion compared with treatment with

  6. Coagulation alterations due to local fibrinolytic therapy with recombinant tissue-type plasminogen activator (rt-PA) in patients with peripheral arterial occlusive disease

    SciTech Connect

    Rauber, Klaus; Heidinger, Kathrin S.; Kemkes-Matthes, Bettina

    1997-05-15

    Purpose. To determine the systemic effects of local fibrinolytic therapy with low-dose recombinant tissue-type plasminogen activator (rt-PA). Methods. Ten patients received intrathrombal infusion of 20 mg rt-PA and heparin for local thrombolysis and had subsequent percutaneous transluminal angioplasty (PTA). Eight controls underwent PTA and received heparin alone. We measured t-PA, D-Dimer, and fibrinogen levels before, directly after, and 20, 40, and 60 min and 24 hr after therapy. Results. In the thrombolysis group the t-PA level peaked immediately after infusion and then declined within 1 hr. D-Dimer increased and remained elevated, whereas in the control group only t-PA levels increased, and only after 24 hr. Fibrinogen remained within the normal range in both groups. Eight of ten patients in the thrombolysis group and seven of eight with PTA had clinical improvement after the procedure. Conclusions. The increase in D-Dimer in the rt-PA group indicates a good local fibrinolytic effect. The fact that fibrinogen levels remained unchanged indicates that there is a lack of systemic fibrinogenolysis.

  7. Expression of the urokinase plasminogen activator receptor (uPAR) and its ligand (uPA) in brain tissues of human immunodeficiency virus patients with opportunistic cerebral diseases.

    PubMed

    Nebuloni, Manuela; Cinque, Paola; Sidenius, Nicolai; Ferri, Angelita; Lauri, Eleonora; Omodeo-Zorini, Elisabetta; Zerbi, Pietro; Vago, Luca

    2009-01-01

    The urokinase plasminogen activator receptor (uPAR) and its ligand (uPA) play an important role in cell migration and extracellular proteolysis. We previously described uPAR/uPA overexpression in the cerebrospinal fluid (CSF) and brain tissues of patients with human immunodeficiency virus (HIV)-related cerebral diseases. In this study, we examined uPAR/uPA expression by immunohistochemistry (IHC) in brains of HIV patients with opportunistic cerebral lesions and in HIV-positive/negative controls. uPAR was found in macrophages/microglia with the highest levels in cytomegalovirus (CMV) encephalitis, toxoplasmosis, and lymphomas; in cryptococcosis and progressive multifocal leukoencephalopathy (PML) cases, only a few positive cells were found and no positivity was observed in controls. uPA expression was demonstrated only in a few macrophages/microglia and lymphocytes in all the cases and HIV-positive controls without different pattern of distribution; no uPA immunostaining was found in cryptococcosis and HIV-negative controls. The higher expression of uPAR/uPA in most of the opportunistic cerebral lesions supports their role in these diseases, suggesting their contribution to tissue injury.

  8. 75 FR 43809 - Airworthiness Directives; Piper Aircraft, Inc. PA-28, PA-32, PA-34, and PA-44 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-27

    .... PA-28, PA-32, PA- 34, and PA-44 Series Airplanes AGENCY: Federal Aviation Administration (FAA), DOT... Aircraft, Inc. (Piper) PA-28, PA-32, PA-34, and PA-44 series airplanes. This AD requires you to inspect the... apply to certain Piper Aircraft, Inc. (Piper) PA-28, PA-32, PA- 34, and PA-44 series airplanes....

  9. Effects of locomotor skill program on minority preschoolers' physical activity levels.

    PubMed

    Alhassan, Sofiya; Nwaokelemeh, Ogechi; Ghazarian, Manneh; Roberts, Jasmin; Mendoza, Albert; Shitole, Sanyog

    2012-08-01

    This pilot study examined the effects of a teacher-taught, locomotor skill (LMS)-based physical activity (PA) program on the LMS and PA levels of minority preschooler-aged children. Eight low-socioeconomic status preschool classrooms were randomized into LMS-PA (LMS-oriented lesson plans) or control group (supervised free playtime). Interventions were delivered for 30 min/day, five days/week for six months. Changes in PA (accelerometer) and LMS variables were assessed with MANCOVA. LMS-PA group exhibited a significant reduction in during-preschool (F (1,16) = 6.34, p = .02, d = 0.02) and total daily (F (1,16) = 9.78, p = .01, d = 0.30) percent time spent in sedentary activity. LMS-PA group also exhibited significant improvement in leaping skills, F (1, 51) = 7.18, p = .01, d = 0.80). No other, significant changes were observed. The implementation of a teacher-taught, LMS-based PA program could potentially improve LMS and reduce sedentary time of minority preschoolers.

  10. Biochemical, thrombolytic and pharmacokinetic properties of rt-PA P47G, K49N, a substitution variant of human tissue-type plasminogen activator.

    PubMed

    Nelles, L; Li, X K; Vanlinthout, I; De Cock, F; Lijnen, H R; Collen, D

    1992-04-02

    rt-PA P47G, K49N, a substitution variant of recombinant human tissue-type plasminogen activator (rt-PA), in which proline at position 47 and lysine at position 49 were replaced by glycine and asparagine respectively, was previously described by Ahern et al. (J Biol Chem 1990; 265:5540-5) to have an extended in vivo half-life with unaltered in vitro fibrinolytic properties. Because this variant might possess an increased in vivo thrombolytic potency, we have constructed its cDNA, expressed it in Chinese hamster ovary cells and determined its biochemical, thrombolytic and pharmacokinetic properties relative to those of home-made rt-PA and of alteplase (Actilyse). The specific fibrinolytic activities on fibrin plates were 160,000 +/- 17,000, 210,000 +/- 88,000 and 460,000 +/- 72,000 IU/mg (mean +/- SEM) for rt-PA P47G, K49N, rt-PA and alteplase, respectively, while the catalytic efficiencies for plasminogen activation (k2/Km) in the absence of fibrin were comparable (1.1 to 1.7 x 10(-3) microM-1s-1). Fibrin enhanced the rate of plasminogen activation by rt-PA P47G, K49N 100-fold and by both wild-type molecules 390-fold. Binding of the variant rt-PA to fibrin was significantly reduced, but its affinity for lysine-Sepharose was unaltered. In an in vitro clot lysis system, consisting of a radiolabeled human plasma clot submersed in plasma, 50% clot lysis in 2 h required 0.67 +/- 0.14 micrograms/ml rt-PA P47G, K49N, 0.36 +/- 0.01 micrograms/ml rt-PA and 0.17 +/- 0.01 micrograms/ml alteplase, respectively (mean +/- SEM; n = 3 or 4). At these doses residual fibrinogen levels at 2 h were in excess of 80%.(ABSTRACT TRUNCATED AT 250 WORDS)

  11. Maduramicin Rapidly Eliminates Malaria Parasites and Potentiates the Gametocytocidal Activity of the Pyrazoleamide PA21A050

    PubMed Central

    Maron, Maxim I.; Magle, Crystal T.; Czesny, Beata; Turturice, Benjamin A.; Huang, Ruili; Zheng, Wei; Vaidya, Akhil B.

    2015-01-01

    New strategies targeting Plasmodium falciparum gametocytes, the sexual-stage parasites that are responsible for malaria transmission, are needed to eradicate this disease. Most commonly used antimalarials are ineffective against P. falciparum gametocytes, allowing patients to continue to be infectious for over a week after asexual parasite clearance. A recent screen for gametocytocidal compounds demonstrated that the carboxylic polyether ionophore maduramicin is active at low nanomolar concentrations against P. falciparum sexual stages. In this study, we showed that maduramicin has an EC50 (effective concentration that inhibits the signal by 50%) of 14.8 nM against late-stage gametocytes and significantly blocks in vivo transmission in a mouse model of malaria transmission. In contrast to other reported gametocytocidal agents, maduramicin acts rapidly in vitro, eliminating gametocytes and asexual schizonts in less than 12 h without affecting uninfected red blood cells (RBCs). Ring stage parasites are cleared by 24 h. Within an hour of drug treatment, 40% of the normally crescent-shaped gametocytes round up and become spherical. The number of round gametocytes increases to >60% by 2 h, even before a change in membrane potential as monitored by MitoProbe DiIC1 (5) is detectable. Maduramicin is not preferentially taken up by gametocyte-infected RBCs compared to uninfected RBCs, suggesting that gametocytes are more sensitive to alterations in cation concentration than RBCs. Moreover, the addition of 15.6 nM maduramicin enhanced the gametocytocidal activity of the pyrazoleamide PA21A050, which is a promising new antimalarial candidate associated with an increase in intracellular Na+ concentration that is proposed to be due to inhibition of PfATP4, a putative Na+ pump. These results underscore the importance of cation homeostasis in sexual as well as asexual intraerythrocytic-stage P. falciparum parasites and the potential of targeting this pathway for drug development

  12. Adherence to a Physical Activity Program by Older Adults in Brazil

    ERIC Educational Resources Information Center

    de Souza, Doralice Lange; Vendruscolo, Rosecler

    2010-01-01

    This paper discusses the results of a qualitative research project in which we investigated adherence factors to a physical activity (PA) program for older adults in Brazil named "Sem Fronteiras: Atividades Corporais Para Adultos Maduros e Idosos", which translated into English means "Without Borders: Physical Activities for Mature…

  13. Overexpression of Hsp27 in a human melanoma cell line: regulation of E-cadherin, MUC18/MCAM, and plasminogen activator (PA) system

    PubMed Central

    Aldrian, Silke; Kindas-Mügge, Ingela; Trautinger, Franz; Fröhlich, Ilse; Gsur, Andrea; Herbacek, Irene; Berger, Walter; Micksche, Michael

    2003-01-01

    Hsp27 is considered a potential marker for cell differentiation in diverse tissues. Several aspects linked to the differentiation process and to the transition from high to low metastatic potential were analyzed in melanoma cells transfected with Hsp27. E-cadherin plays a central role in cell differentiation, migration, and normal development. Loss of expression or function of E-cadherin has been documented in a variety of human malignancies. We observed by fluorescence-activated cell sorter (FACS) as well as immunofluorescence (IF) analysis a pronounced expression of E-cadherin in Hsp27-transfected A375 melanoma cells compared with control melanoma cells. The expression of the adhesion molecule MUC18/MCAM correlates directly with the metastatic potential of melanoma cells. In contrast to wild-type and neotransfected melanoma cells, in Hsp27-transfected cells the expression of MUC18/MCAM could not be detected by FACS and IF analysis. The plasminogen activator (PA) system plays a central role in mediating extracellular proteolysis and also in nonproteolytic events such as cell adhesion, migration, and transmembrane signaling. Hsp27 transfectants revealed elevated messenger ribonucleic acid expression of the urokinase-type PA (uPA) and its inhibitor, PA inhibitor type 1, which might indicate a neutralization effect of the proteolytic activity of uPA. Control cells failed to express both these molecules. The influence of Hsp27 expression on uPA activity and the involvement of E-cadherin could be demonstrated by use of anti–E-cadherin–blocking antibody. Our data provide evidence for an inhibitory-regulatory role of Hsp27 in tumor progression as found in our system. PMID:14984058

  14. Genome-Wide Association Study for Circulating Tissue Plasminogen Activator (tPA) Levels and Functional Follow-up Implicates Endothelial STXBP5 and STX2

    PubMed Central

    Huang, Jie; Huffman, Jennifer E.; Yamkauchi, Munekazu; Trompet, Stella; Asselbergs, Folkert W.; Sabater-Lleal, Maria; Trégouët, David-Alexandre; Chen, Wei-Min; Smith, Nicholas L.; Kleber, Marcus E.; Shin, So-Youn; Becker, Diane M.; Tang, Weihong; Dehghan, Abbas; Johnson, Andrew D.; Truong, Vinh; Folkersen, Lasse; Yang, Qiong; Oudot-Mellakh, Tiphaine; Buckley, Brendan M.; Moore, Jason H.; Williams, Frances M.K.; Campbell, Harry; Silbernagel, Günther; Vitart, Veronique; Rudan, Igor; Tofler, Geoffrey H.; Navis, Gerjan J.; DeStefano, Anita; Wright, Alan F.; Chen, Ming-Huei; de Craen, Anton J.M.; Worrall, Bradford B.; Rudnicka, Alicja R.; Rumley, Ann; Bookman, Ebony B.; Psaty, Bruce M.; Chen, Fang; Keene, Keith L.; Franco, Oscar H.; Böhm, Bernhard O.; Uitterlinden, Andre G.; Carter, Angela M.; Jukema, J. Wouter; Sattar, Naveed; Bis, Joshua C.; Ikram, Mohammad A.; Sale, Michèle M.; McKnight, Barbara; Fornage, Myriam; Ford, Ian; Taylor, Kent; Slagboom, P. Eline; McArdle, Wendy L.; Hsu, Fang-Chi; Franco-Cereceda, Anders; Goodall, Alison H.; Yanek, Lisa R.; Furie, Karen L.; Cushman, Mary; Hofman, Albert; Witteman, Jacqueline CM.; Folsom, Aaron R.; Basu, Saonli; Matijevic, Nena; van Gilst, Wiek H.; Wilson, James F.; Westendorp, Rudi G.J.; Kathiresan, Sekar; Reilly, Muredach P.; Tracy, Russell P.; Polasek, Ozren; Winkelmann, Bernhard R.; Grant, Peter J.; Hillege, Hans L.; Cambien, Francois; Stott, David J.; Lowe, Gordon D.; Spector, Timothy D.; Meigs, James B.; Marz, Winfried; Eriksson, Per; Becker, Lewis C.; Morange, Pierre-Emmanuel; Soranzo, Nicole; Williams, Scott M.; Hayward, Caroline; van der Harst, Pim; Hamsten, Anders; Lowenstein, Charles J.; Strachan, David P.; O'Donnell, Christopher J.

    2014-01-01

    Objective Tissue plasminogen activator (tPA), a serine protease, catalyzes the conversion of plasminogen to plasmin, the major enzyme responsible for endogenous fibrinolysis. In some populations, elevated plasma levels of tPA have been associated with myocardial infarction and other cardiovascular diseases (CVD). We conducted a meta-analysis of genome-wide association studies (GWAS) to identify novel correlates of circulating levels of tPA. Approach and Results Fourteen cohort studies with tPA measures (N=26,929) contributed to the meta-analysis. Three loci were significantly associated with circulating tPA levels (P <5.0×10−8). The first locus is on 6q24.3, with the lead SNP (rs9399599, P=2.9×10−14) within STXBP5. The second locus is on 8p11.21. The lead SNP (rs3136739, P=1.3×10−9) is intronic to POLB and less than 200kb away from the tPA encoding gene PLAT. We identified a non-synonymous SNP (rs2020921) in modest LD with rs3136739 (r2 = 0.50) within exon 5 of PLAT (P=2.0×10−8). The third locus is on 12q24.33, with the lead SNP (rs7301826, P=1.0×10−9) within intron 7 of STX2. We further found evidence for association of lead SNPs in STXBP5 and STX2 with expression levels of the respective transcripts. In in vitro cell studies, silencing STXBP5 decreased release of tPA from vascular endothelial cells, while silencing of STX2 increased tPA release. Through an in-silico lookup, we found no associations of the three lead SNPs with coronary artery disease or stroke. Conclusions We identified three loci associated with circulating tPA levels, the PLAT region, STXBP5 and STX2. Our functional studies implicate a novel role for STXBP5 and STX2 in regulating tPA release. PMID:24578379

  15. National Dam Inspection Program. Crabapple Dam (NDI Number PA 00907, PennDER Number 26-32) Ohio River Basin, Crabapple Run, Fayette County, Pennsylvania. Phase I Inspection Report,

    DTIC Science & Technology

    1980-07-01

    7 D-A091 529 ACKENHEIL AND ASSOCIATES INC PITTSBURGH PA F/6 13/13 NATIONAL DAM INSPECTION PROGRAM. CRABAPPLE DAM (NDI NUMBER PA 0-ETC(U) JUL B8 J P...HANNAN, J E BARRICK DAC 31- BOC-0026 UNCLASSIFIE NLE2IIIIIIIhIIIl EIIIIIIIIIIIII 0OHI0 RIVER BASIN CRABAPPLE RUN FAYETTE COUNTY PENNSYLVANIA NDI No. PA...00907 :E- 1rŘ PENN DER No. 26-32 me CRABAPPLE DAM STEPHEN VINCINSKI and GEORGE THOMAS PHASE I INSPECTION REPORT NATIONAL DAM INSPECTION PROGRAM J 1

  16. Iron oxide nanoparticles protected by NIR-active multidentate-polymers as multifunctional nanoprobes for NIRF/PA/MR trimodal imaging.

    PubMed

    Wu, Yayun; Gao, Duyang; Zhang, Pengfei; Li, Chuansheng; Wan, Qian; Chen, Chi; Gong, Ping; Gao, Guanhui; Sheng, Zonghai; Cai, Lintao

    2016-01-14

    We designed and synthesized new kinds of near-infrared catechol-based multidentate polymers which were intended to yield compact NIR-active iron oxide nanoparticles with excellent stability and biocompatibility. The resulted multifunctional nanoprobes showed great potential as multimodal contrast agents for NIRF/PA/MR trimodal imaging in vivo.

  17. Tissue-type Plasminogen Activator (tPA) Modulates the Postsynaptic Response of Cerebral Cortical Neurons to the Presynaptic Release of Glutamate

    PubMed Central

    Jeanneret, Valerie; Wu, Fang; Merino, Paola; Torre, Enrique; Diaz, Ariel; Cheng, Lihong; Yepes, Manuel

    2016-01-01

    Tissue-type plasminogen activator (tPA) is a serine proteinase released by the presynaptic terminal of cerebral cortical neurons following membrane depolarization (Echeverry et al., 2010). Recent studies indicate that the release of tPA triggers the synaptic vesicle cycle and promotes the exocytosis (Wu et al., 2015) and endocytic retrieval (Yepes et al., 2016) of glutamate-containing synaptic vesicles. Here we used electron microscopy, proteomics, quantitative phosphoproteomics, biochemical analyses with extracts of the postsynaptic density (PSD), and an animal model of cerebral ischemia with mice overexpressing neuronal tPA to study whether the presynaptic release of tPA also has an effect on the postsynaptic terminal. We found that tPA has a bidirectional effect on the composition of the PSD of cerebral cortical neurons that is independent of the generation of plasmin and the presynaptic release of glutamate, but depends on the baseline level of neuronal activity and the extracellular concentrations of calcium (Ca2+). Accordingly, in neurons that are either inactive or incubated with low Ca2+ concentrations tPA induces phosphorylation and accumulation in the PSD of the Ca2+/calmodulin-dependent protein kinase IIα (pCaMKIIα), followed by pCaMKIIα-mediated phosphorylation and synaptic recruitment of GluR1-containing α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors. In contrast, in neurons with previously increased baseline levels of pCaMKIIα in the PSD due to neuronal depolarization in vivo or incubation with high concentrations of either Ca2+ or glutamate in vitro, tPA induces pCaMKIIα and pGluR1 dephosphorylation and their subsequent removal from the PSD. We found that these effects of tPA are mediated by synaptic N-methyl-D-aspartate (NMDA) receptors and cyclin-dependent kinase 5 (Cdk5)-induced phosphorylation of the protein phosphatase 1 (PP1) at T320. Our data indicate that by regulating the pCaMKIIα/PP1 balance in the PSD tPA acts

  18. Effects of an Intensive Street-Level Police Intervention on Syringe Exchange Program Use in Philadelphia, Pa

    PubMed Central

    Davis, Corey S.; Burris, Scott; Kraut-Becher, Julie; Lynch, Kevin G.; Metzger, David

    2005-01-01

    Repeated measurements and mixed-effects models were used to analyze the effects of an intensive long-term street-level police intervention on syringe exchange program use. Utilization data for 9 months before and after the beginning of the intervention were analyzed. Use fell across all categories and time periods studied, with significant declines in use among total participants, male participants, and Black participants. Declines in use among Black and male participants were much more pronounced than decreases among White and female participants. PMID:15671455

  19. Dropout Prevention/Reduction Programs and Activities.

    ERIC Educational Resources Information Center

    Dade County Public Schools, Miami, FL.

    Seventeen activities or programs conducted in the Dade County (Florida) public elementary and secondary schools in order to reduce or prevent dropout are described in this resource guide. The programs activities include: (1) workshops to develop school-based dropout prevention programs; (2) "Students Working Intelligently to Combat High…

  20. Building Technologies Program Key Activities

    SciTech Connect

    2011-12-15

    The Building Technologies Program (BTP) employs a balanced approach to making buildings more energy efficient. The three pillars of our program, research and development (R&D), market stimulation, and building and equipment standards, help meet our strategic vision.

  1. Synergistic transcriptional activation of the mouse urokinase plasminogen activator (uPA) gene and of its enhancer activator protein 1 (AP1) site by cAMP and retinoic acid.

    PubMed Central

    Mira-Y-Lopez, R; Jaramillo, S; Jing, Y

    1998-01-01

    We have investigated the mechanism whereby all-trans retinoic acid (tRA) potentiates the 8-bromo-cAMP (8-BrcAMP)-dependent transcription of the urokinase plasminogen activator (uPA) gene in SC115 mouse mammary carcinoma cells. Photoaffinity labelling experiments showed that tRA did not alter the cellular content of cAMP-dependent protein kinase regulatory subunits I and II. In agreement with this, nuclear run-on analysis in the presence of the translational inhibitor puromycin demonstrated that the effect of 8-BrcAMP and its potentiation by tRA were independent of protein synthesis. A transiently transfected 6.6 kb uPA 5'-flanking region-chloramphenicol acetyltransferase (CAT) fusion gene mimicked the response of the endogenous uPA gene. Thus 1 mM 8-BrcAMP induced a 100-200% increase in CAT content, 100 nM tRA had no effect and 100 nM tRA+1 mM 8-BrcAMP induced a 300-500% increase in cells co-transfected with tRA receptor and/or 9-cis-RA receptor. Analysis of 5'-deleted constructs showed that the tRA effect required at least two cis regions: -2657 to -2186, encompassing the 100 bp uPA enhancer, and -709 to -324, which exhibited silencing activity. Neither region contained a tRA-response element-like motif. Because tRA receptor and 9-cis-RA receptor interact with activator protein 1 (AP1), we tested whether tRA regulated the uPA enhancer AP1 site in the presence of 8-BrcAMP. We found that a dimer of this site fused to a minimal uPA-CAT fusion gene was responsive to 1 mM 8-BrcAMP (100% CAT increase), not responsive to 100 nM tRA, and synergistically responsive to 100 nM tRA+1 mM 8-BrcAMP (240% CAT increase) in cells co-transfected with Fos and Jun. Synergistic activation of the same construct and of the 6.6 kb uPA-CAT fusion gene was also obtained using tRA and 100 nM PMA. We conclude that multiple cis elements, probably including the uPA enhancer AP1 site, mediate the tRA potentiation of uPA transcription. PMID:9560322

  2. PaR-PaR laboratory automation platform.

    PubMed

    Linshiz, Gregory; Stawski, Nina; Poust, Sean; Bi, Changhao; Keasling, Jay D; Hillson, Nathan J

    2013-05-17

    Labor-intensive multistep biological tasks, such as the construction and cloning of DNA molecules, are prime candidates for laboratory automation. Flexible and biology-friendly operation of robotic equipment is key to its successful integration in biological laboratories, and the efforts required to operate a robot must be much smaller than the alternative manual lab work. To achieve these goals, a simple high-level biology-friendly robot programming language is needed. We have developed and experimentally validated such a language: Programming a Robot (PaR-PaR). The syntax and compiler for the language are based on computer science principles and a deep understanding of biological workflows. PaR-PaR allows researchers to use liquid-handling robots effectively, enabling experiments that would not have been considered previously. After minimal training, a biologist can independently write complicated protocols for a robot within an hour. Adoption of PaR-PaR as a standard cross-platform language would enable hand-written or software-generated robotic protocols to be shared across laboratories.

  3. PaR-PaR Laboratory Automation Platform

    SciTech Connect

    Linshiz, G; Stawski, N; Poust, S; Bi, CH; Keasling, JD; Hilson, NJ

    2013-05-01

    Labor-intensive multistep biological tasks, such as the construction and cloning of DNA molecules, are prime candidates for laboratory automation. Flexible and biology-friendly operation of robotic equipment is key to its successful integration in biological laboratories, and the efforts required to operate a robot must be much smaller than the alternative manual lab work. To achieve these goals, a simple high-level biology-friendly robot programming language is needed. We have developed and experimentally validated such a language: Programming a Robot (PaR-PaR). The syntax and compiler for the language are based on computer science principles and a deep understanding of biological workflows. PaR-PaR allows researchers to use liquid-handling robots effectively, enabling experiments that would not have been considered previously. After minimal training, a biologist can independently write complicated protocols for a robot within an hour. Adoption of PaR-PaR as a standard cross-platform language would enable hand-written or software-generated robotic protocols to be shared across laboratories.

  4. Using the RE-AIM framework to evaluate physical activity public health programs in Mexico

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Physical activity (PA) public health programming has been widely used in Mexico; however, few studies have documented individual and organizational factors that might be used to evaluate their public health impact. The RE-AIM framework is an evaluation tool that examines individual and organizationa...

  5. National Dam Inspection Program. Lake of the Four Seasons Dam (NDS-ID Number PA-568, DER-ID Number 40-225) Susquehanna River Basin, Oley Creek, Luzerne County, Pennsylvania. Phase I Inspection Report,

    DTIC Science & Technology

    1980-09-01

    A0-AU91 266 KIMBALL (L ROBERT) AND ASSOCIATES EBENSBURG PA F/G 13/13 NATIONAL JAM INSPECTION PROGRAM. LAKE OF THE FOUR SEASONS DAM (--ETC(U) SEP 80 R...PENNSYLVANI LAKE OF THE FOUR SEASONS DA NDS ID NO. PA-568 DER ID NO. 40-225 DIVERSIFIED MORTGAGE INVESTORS INC. PHASE I INSPECTION REPORT v- NATIONAL DAM...OLEY CREEK LUZERNE COUNTY ~ IEPEN NSYL VAN IA-,, LAKE OF THE FOUR SEASONS DAM li0 L __NOS4D14. PA-56! DER 40. 4Y225) A:HASE JINSPE TIO LEP 5 NATIONAL

  6. Change in energy expenditure and physical activity in response to aerobic and resistance exercise programs.

    PubMed

    Drenowatz, Clemens; Grieve, George L; DeMello, Madison M

    2015-01-01

    Exercise is considered an important component of a healthy lifestyle but there remains controversy on effects of exercise on non-exercise physical activity (PA). The present study examined the prospective association of aerobic and resistance exercise with total daily energy expenditure and PA in previously sedentary, young men. Nine men (27.0 ± 3.3 years) completed two 16-week exercise programs (3 exercise sessions per week) of aerobic and resistance exercise separated by a minimum of 6 weeks in random order. Energy expenditure and PA were measured with the SenseWear Mini Armband prior to each intervention as well as during week 1, week 8 and week 16 of the aerobic and resistance exercise program. Body composition was measured via dual x-ray absorptiometry. Body composition did not change in response to either exercise intervention. Total daily energy expenditure on exercise days increased by 443 ± 126 kcal/d and 239 ± 152 kcal/d for aerobic and resistance exercise, respectively (p < 0.01). Non-exercise moderate-to-vigorous PA, however, decreased on aerobic exercise days (-148 ± 161 kcal/d; p = 0.03). There was no change in total daily energy expenditure and PA on non-exercise days with aerobic exercise while resistance exercise was associated with an increase in moderate-to-vigorous PA during non-exercise days (216 ± 178 kcal/d, p = 0.01). Results of the present study suggest a compensatory reduction in PA in response to aerobic exercise. Resistance exercise, on the other hand, appears to facilitate non-exercise PA, particularly on non-exercise days, which may lead to more sustainable adaptations in response to an exercise program.

  7. National Dam Inspection Program, Big Beaver Dam (Pa No Name Number 14, NDI Number PA 00258, PennDER Number 4-11), Ohio River Basin, Tributary of Clark’s Run, Beaver County, Pennsylvania. Phase I Inspection Report,

    DTIC Science & Technology

    1980-02-01

    the Allegheny Group , Pennsylvanian System. These members consist of cyclic sequences of shale, sandstone, limestone, and coal. It is estimated that the...4, GEOLOGIC MAP Blg Beaver Dom NDI No. PA 00258 Beaver County c Reproduced from Greater Pittsburgh Region Geologic Map. -A% 0 4 Compiled by W. R...Wagner and others, 1975 N Scale: One Inch Equals Approximately Two Miles See Legend, Next Page GEOLOGY MAP LEGEND GROUP FORMATION DESCRIPTION Alluvium

  8. Iron oxide nanoparticles protected by NIR-active multidentate-polymers as multifunctional nanoprobes for NIRF/PA/MR trimodal imaging

    NASA Astrophysics Data System (ADS)

    Wu, Yayun; Gao, Duyang; Zhang, Pengfei; Li, Chuansheng; Wan, Qian; Chen, Chi; Gong, Ping; Gao, Guanhui; Sheng, Zonghai; Cai, Lintao

    2015-12-01

    We designed and synthesized new kinds of near-infrared catechol-based multidentate polymers which were intended to yield compact NIR-active iron oxide nanoparticles with excellent stability and biocompatibility. The resulted multifunctional nanoprobes showed great potential as multimodal contrast agents for NIRF/PA/MR trimodal imaging in vivo.We designed and synthesized new kinds of near-infrared catechol-based multidentate polymers which were intended to yield compact NIR-active iron oxide nanoparticles with excellent stability and biocompatibility. The resulted multifunctional nanoprobes showed great potential as multimodal contrast agents for NIRF/PA/MR trimodal imaging in vivo. Electronic supplementary information (ESI) available: Details of the experiment and Fig. S1-S6. See DOI: 10.1039/c5nr06660c

  9. PtrA Is Functionally Intertwined with GacS in Regulating the Biocontrol Activity of Pseudomonas chlororaphis PA23

    PubMed Central

    Shah, Nidhi; Klaponski, Natasha; Selin, Carrie; Rudney, Rachel; Fernando, W. G. Dilantha; Belmonte, Mark F.; de Kievit, Teresa R.

    2016-01-01

    In vitro inhibition of the fungal pathogen Sclerotinia sclerotiorum by Pseudomonas chlororaphis PA23 is reliant upon a LysR-type transcriptional regulator (LTTR) called PtrA. In the current study, we show that Sclerotinia stem rot and leaf infection are significantly increased in canola plants inoculated with the ptrA-mutant compared to the wild type, establishing PtrA as an essential regulator of PA23 biocontrol. LTTRs typically regulate targets that are upstream of and divergently transcribed from the LTTR locus. We identified a short chain dehydrogenase (scd) gene immediately upstream of ptrA. Characterization of a scd mutant revealed that it is phenotypically identical to the wild type. Moreover, scd transcript abundance was unchanged in the ptrA mutant. These findings indicate that PtrA regulation does not involve scd, rather this LTTR controls genes located elsewhere on the chromosome. Employing a combination of complementation and transcriptional analysis we investigated whether connections exist between PtrA and other regulators of biocontrol. Besides ptrA, gacS was the only gene able to partially rescue the wild-type phenotype, establishing a connection between PtrA and the sensor kinase GacS. Transcriptomic analysis revealed decreased expression of biosynthetic (phzA, prnA) and regulatory genes (phzI, phzR, rpoS, gacA, rsmX, rsmZ, retS) in the ptrA mutant; conversely, rsmE, and rsmY were markedly upregulated. The transcript abundance of ptrA was nine-fold higher in the mutant background indicating that this LTTR negatively autoregulates itself. In summary, PtrA is an essential regulator of genes required for PA23 biocontrol that is functionally intertwined with GacS. PMID:27713742

  10. 26S and PA28-20S Proteasome Activity in Cytosolic Extracts from Embryonic Stem Cells.

    PubMed

    Hernebring, Malin

    2016-01-01

    The proteasome is a complex multisubunit protease that plays a major role in the degradation of proteins in eukaryotic cells. Proteasome function is one of the key players regulating the proteome and it is vital for many cellular processes. The method described here makes it possible to assay the proteolytic capacities of proteasome complexes separately in crude cytosolic extracts from ES cells. The method is based on hydrolysis of a fluorogenic peptide substrate in lysates prepared under conditions that favor the interactions of the 20S proteasomal catalytical core with either the 19S or the PA28αβ proteasome regulator.

  11. Wilderness Adventure Programs: An Activity Profile.

    ERIC Educational Resources Information Center

    Lowenstein, Daniel H.

    Focusing upon the adventure aspect of wilderness programs, this paper presents a profile of those program activities which create a number of challenges and often stressful situations as the means of attaining specified goals and which can best be incorporated under the term "Wilderness Adventure Program" (WAP). Providing information of…

  12. Human Development Program: Level V Activity Guide.

    ERIC Educational Resources Information Center

    Ball, Geraldine

    The curriculum guide presents the activities component of the Human Development Program for grade 5. The Human Development Program (HDP) is an affective curricular approach developed by psychologists to help teachers instill responsibility and self-confidence in children. The activity guide presents topics and directions for 180 sequential Human…

  13. Creating a Before-School Physical Activity Program: Pre-Service Physical Educators' Experiences and Implications for PETE

    ERIC Educational Resources Information Center

    McMullen, Jaimie; van der Mars, Hans; Jahn, Julie A.

    2014-01-01

    The purpose of this study is to describe the experiences of physical education teacher education (PETE) majors enrolled in an internship course that provided them with authentic experiences promoting and facilitating a before-school physical activity (PA) program and to examine the associated implications for PETE programs within the Comprehensive…

  14. Effects of a Movement Integration Program on Elementary School Children's Physical Activity, Fitness Levels, and On-Task Behavior and Teachers' Implementation Experiences

    ERIC Educational Resources Information Center

    Goh, Tan Leng

    2014-01-01

    Physical inactivity is an increasing problem among children and adolescents. The TAKE 10!® program, which integrates physical activity (PA) into academic subjects, was implemented in one elementary school in Magna, Utah to increase children's daily in-school PA. A three-study approach was used to examine: (a) effects of TAKE 10!® on elementary…

  15. Effects of a Physical Activity Program on Markers of Endothelial Dysfunction, Oxidative Stress, and Metabolic Status in Adolescents with Metabolic Syndrome

    PubMed Central

    Camarillo-Romero, Eneida; Dominguez-Garcia, Ma Victoria; Amaya-Chavez, Araceli; Camarillo-Romero, Maria del Socorro; Talavera-Piña, Juan; Huitron-Bravo, Gerardo; Majluf-Cruz, Abraham

    2012-01-01

    The metabolic syndrome (MetS) is a precursor of diabetes. Physical activity (PA) improves endothelial dysfunction and may benefit patients with MetS. Aims. To evaluate the effect of a physical activity (PA) program on markers of endothelial dysfunction and oxidative stress in adolescents with (MetS). Methods. We carried out a cohort study of 38 adolescents with and without MetS (18 females and 20 males). All participants completed a 3-month PA program. All variables of the MetS as well as markers of endothelial dysfunction and oxidative stress tests were evaluated. Results. Females with and without MetS showed significant differences for almost all components of the MetS, whereas males were significantly different in half of the components. After the PA program, components of the MetS were not different from baseline values except for HDL-C levels. Some baseline endothelial dysfunction markers were significantly different among adolescents with and without MetS; however, after the PA program, most of these markers significantly improved in subjects with and without MetS. Conclusion. PA improves the markers of endothelial dysfunction in adolescents with MetS although other changes in the components of the MetS were not observed. Perhaps the benefits of PA on all components of MetS would appear after a PA program with a longer duration. PMID:22888450

  16. Vehicle Technologies Program Educational Activities

    SciTech Connect

    2011-12-13

    Description of educational activities including: EcoCAR2: Plugging In to the Future, EcoCAR: The NeXt Challenge, Green Racing, Automotive X Prize, Graduate Technology Automotive Education (GATE), and Hydrogen Education.

  17. Feasibility Study of Comprehensive School Physical Activity Programs in Appalachian Communities: The McDowell CHOICES Project

    ERIC Educational Resources Information Center

    Jones, Emily M.; Taliaferro, Andrea R.; Elliott, Eloise M.; Bulger, Sean M.; Kristjansson, Alfgeir L.; Neal, William; Allar, Ishonté

    2014-01-01

    Increasing rates of childhood obesity has prompted calls for comprehensive approaches to school-based physical activity (PA). The purpose of this study was to evaluate the feasibility of comprehensive school physical activity program (CSPAP) development and related contextual issues within a rural Appalachian county using a Systems Approach. A…

  18. Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus

    PubMed Central

    Guzmán-Rodríguez, Jaquelina Julia; López-Gómez, Rodolfo; Suárez-Rodríguez, Luis M.; Salgado-Garciglia, Rafael; Rodríguez-Zapata, Luis C.; Ochoa-Zarzosa, Alejandra; López-Meza, Joel E.

    2013-01-01

    Antimicrobial therapy is a useful tool to control infectious diseases in general and rising antibiotic resistant microorganisms in particular. Alternative strategies are desirable, and antimicrobial peptides (AMP) represent attractive control agents. Mexican avocado (Persea americana var. drymifolia) is used in traditional medicine; however, the AMP production has not been reported in this plant. We obtained a cDNA library from avocado fruit and clone PaDef was identified, which has a cDNA (249 bp) encoding a protein (78 aa) homologous with plant defensins (>80%). We expressed the defensin PaDef cDNA (pBME3) in the bovine endothelial cell line BVE-E6E7. Polyclonal and clonal populations were obtained and their activity was evaluated against Escherichia coli, Staphylococcus aureus, and Candida albicans. E. coli viability was inhibited with 100 μg/mL of total protein from clones (>55%). Also, S. aureus viability was inhibited from 50 μg/mL total protein (27–38%) but was more evident at 100 μg/mL (52–65%). This inhibition was higher than the effect showed by polyclonal population (~23%). Finally, we did not detect activity against C. albicans. These results are the first report that shows antimicrobial activity of a defensin produced by avocado and suggest that this AMP could be used in the control of pathogens. PMID:24319695

  19. Defense activation triggers differential expression of phospholipase-C (PLC) genes and elevated temperature induces phosphatidic acid (PA) accumulation in tomato

    PubMed Central

    Abd-El-Haliem, Ahmed; Meijer, Harold J.G.; Tameling, Wladimir I.L.; Vossen, Jack H.; Joosten, Matthieu H.A.J.

    2012-01-01

    Recently, we provided the first genetic evidence for the requirement of tomato PLC4 and PLC6 genes in defense activation and disease resistance. The encoded enzymes were catalytically active as they were able to degrade phosphatidylinositol (PI), thereby producing diacylglycerol (DG). Here we report differential PLC gene expression following the initiation of defense signaling by the interaction between Cladosporium fulvum resistance (R) protein Cf-4 and its matching effector Avr4 in tomato hybrid seedlings that express both Cf-4 and Avr4. Furthermore, we observed that PLC3 and PLC6 gene expression is upregulated by elevated temperature in the control seedlings. This upregulation coincides with an increase in the levels of phosphatidic acid (PA) and a decrease in the levels of PI and phosphatidylinositol phosphate (PIP). The decrease in PI and PIP levels matches with the activation of PLC. In addition, the levels of the structural phospholipids phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) declined transiently during recovery after the exposure to elevated temperature., Further studies will be required to explain the mechanism causing the sustained accumulation of PA during recovery, combined with a reduction in the levels of structural phospholipids. PMID:22899083

  20. Antibacterial activity of defensin PaDef from avocado fruit (Persea americana var. drymifolia) expressed in endothelial cells against Escherichia coli and Staphylococcus aureus.

    PubMed

    Guzmán-Rodríguez, Jaquelina Julia; López-Gómez, Rodolfo; Suárez-Rodríguez, Luis M; Salgado-Garciglia, Rafael; Rodríguez-Zapata, Luis C; Ochoa-Zarzosa, Alejandra; López-Meza, Joel E

    2013-01-01

    Antimicrobial therapy is a useful tool to control infectious diseases in general and rising antibiotic resistant microorganisms in particular. Alternative strategies are desirable, and antimicrobial peptides (AMP) represent attractive control agents. Mexican avocado (Persea americana var. drymifolia) is used in traditional medicine; however, the AMP production has not been reported in this plant. We obtained a cDNA library from avocado fruit and clone PaDef was identified, which has a cDNA (249 bp) encoding a protein (78 aa) homologous with plant defensins (>80%). We expressed the defensin PaDef cDNA (pBME3) in the bovine endothelial cell line BVE-E6E7. Polyclonal and clonal populations were obtained and their activity was evaluated against Escherichia coli, Staphylococcus aureus, and Candida albicans. E. coli viability was inhibited with 100 μg/mL of total protein from clones (>55%). Also, S. aureus viability was inhibited from 50 μg/mL total protein (27-38%) but was more evident at 100 μg/mL (52-65%). This inhibition was higher than the effect showed by polyclonal population (~23%). Finally, we did not detect activity against C. albicans. These results are the first report that shows antimicrobial activity of a defensin produced by avocado and suggest that this AMP could be used in the control of pathogens.

  1. Technical Insights for Saltstone PA Maintenance

    SciTech Connect

    Flach, G.; Sarkar, S.; Mahadevan, S.; Kosson, D.

    2011-07-20

    The Cementitious Barriers Partnership (CBP) is a collaborative program sponsored by the US DOE Office of Waste Processing. The objective of the CBP is to develop a set of computational tools to improve understanding and prediction of the long-term structural, hydraulic, and chemical performance of cementitious barriers and waste forms used in nuclear applications. CBP tools are expected to better characterize and reduce the uncertainties of current methodologies for assessing cementitious barrier performance and increase the consistency and transparency of the assessment process, as the five-year program progresses. In September 2009, entering its second year of funded effort, the CBP sought opportunities to provide near-term tangible support to DOE Performance Assessments (PAs). The Savannah River Saltstone Disposal Facility (SDF) was selected for the initial PA support effort because (1) cementitious waste forms and barriers play a prominent role in the performance of the facility, (2) certain important long-term behaviors of cementitious materials composing the facility are uncertain, (3) review of the SDF PA by external stakeholders is ongoing, and (4) the DOE contractor responsible for the SDF PA is open to receiving technical assistance from the CBP. A review of the current (SRR Closure & Waste Disposal Authority 2009) and prior Saltstone PAs (e.g., Cook et al. 2005) suggested five potential opportunities for improving predictions. The candidate topics considered were (1) concrete degradation from external sulfate attack, (2) impact of atmospheric exposure to concrete and grout before closure, such as accelerated slag and Tc-99 oxidation, (3) mechanistic prediction of geochemical conditions, (4) concrete degradation from rebar corrosion due to carbonation, and (5) early age cracking from drying and/or thermal shrinkage. The candidate topics were down-selected considering the feasibility of addressing each issue within approximately six months, and

  2. Physical activity opportunities in afterschool programs.

    PubMed

    Weaver, R Glenn; Beets, Michael W; Huberty, Jennifer; Freedman, Darcy; Turner-Mcgrievy, Gabrielle; Ward, Diane

    2015-05-01

    Afterschool programs (ASPs) have potential to provide children moderate to vigorous physical activity (MVPA). The availability and types (e.g., free play or organized activities) of physical activity opportunities, their structure (e.g., presence of lines, elimination games), and staff behaviors (e.g., encouragement, engaged) can influence children's MVPA. This study explored these factors in 20 ASPs serving over 1,700 elementary-age children. The occurrence, types, and structure of physical activity opportunities, and staff behaviors were collected via the SOSPAN (System for Observing Staff Promotion of Physical Activity and Nutrition). A total of 4,660 SOSPAN scans were completed across 63 complete program days (1,733 during physical activity opportunities). Physical activity opportunities were observed on 60 program days across all 20 sites, with 73% of those opportunities classified as free play. ASPs scheduled an average of 66.3 minutes (range 15-150 minutes) of physical activity opportunities daily. Games played included basketball, tag, soccer, and football. Staff rarely engaged in physical activity promotion behaviors, and the structure of organized games discouraged MVPA. For example, staff verbally promoted physical activity in just 6.1% of scans, while organized games were more likely to involve lines and elimination. Professional development training may enhance staffs' physical activity promotion and the structure of activity opportunities.

  3. Physical activity opportunities in afterschool programs

    PubMed Central

    Weaver, R. Glenn; Beets, Michael W.; Huberty, Jennifer; Freedman, Darcy; Turner-Mcgrievy, Gabrielle; Ward, Diane

    2015-01-01

    Afterschool programs (ASPs) have potential to provide children moderate-to-vigorous physical activity (MVPA). The availability and types (e.g., free play or organized activities) of physical activity opportunities, their structure (e.g., presence of lines, elimination games), and staff behaviors (e.g., encouragement, engaged) can influence children’s MVPA. This study explored these factors in 20 ASPs serving over 1,700 elementary-age children. The occurrence, types and structure of physical activity opportunities, and staff behaviors were collected via the System for Observing Staff Promotion of Physical Activity and Nutrition (SOSPAN). A total of 4,660 SOSPAN scans were completed across 63 complete program days (1733 during physical activity opportunities). Physical activity opportunities were observed on 60 program days across all 20 sites, with 73% of those opportunities classified as free play. ASPs scheduled an average of 66.3 minutes (range 15-150min) of physical activity opportunities daily. Games played included basketball, tag, soccer and football. Staff rarely engaged in physical activity promotion behaviors, and the structure of organized games discouraged MVPA. For example, staff verbally promoted physical activity in just 6.1% of scans, while organized games were more likely to involve lines and elimination. Professional development training may enhance staffs’ physical activity promotion and the structure of activity opportunities. PMID:25586132

  4. Activity-Centric Approach to Distributed Programming

    NASA Technical Reports Server (NTRS)

    Levy, Renato; Satapathy, Goutam; Lang, Jun

    2004-01-01

    The first phase of an effort to develop a NASA version of the Cybele software system has been completed. To give meaning to even a highly abbreviated summary of the modifications to be embodied in the NASA version, it is necessary to present the following background information on Cybele: Cybele is a proprietary software infrastructure for use by programmers in developing agent-based application programs [complex application programs that contain autonomous, interacting components (agents)]. Cybele provides support for event handling from multiple sources, multithreading, concurrency control, migration, and load balancing. A Cybele agent follows a programming paradigm, called activity-centric programming, that enables an abstraction over system-level thread mechanisms. Activity centric programming relieves application programmers of the complex tasks of thread management, concurrency control, and event management. In order to provide such functionality, activity-centric programming demands support of other layers of software. This concludes the background information. In the first phase of the present development, a new architecture for Cybele was defined. In this architecture, Cybele follows a modular service-based approach to coupling of the programming and service layers of software architecture. In a service-based approach, the functionalities supported by activity-centric programming are apportioned, according to their characteristics, among several groups called services. A well-defined interface among all such services serves as a path that facilitates the maintenance and enhancement of such services without adverse effect on the whole software framework. The activity-centric application-program interface (API) is part of a kernel. The kernel API calls the services by use of their published interface. This approach makes it possible for any application code written exclusively under the API to be portable for any configuration of Cybele.

  5. Psychosocial Predictors of Changes in Adolescent Girls' Physical Activity and Dietary Behaviors over the Course of the "Go Girls!" Group-Based Mentoring Program

    ERIC Educational Resources Information Center

    Dowd, A. Justine; Chen, Michelle Y.; Schmader, Toni; Jung, Mary E.; Zumbo, Bruno D.; Beauchamp, Mark R.

    2016-01-01

    Changes in social cognitions targeted within a group-based mentoring program for adolescent girls were examined as predictors of changes in physical activity (PA) and dietary behavior (in two separate models) over the course of the 7-week program. Data were collected from 310 participants who participated in the program. Multilevel path models…

  6. Field Operations Program Activities Status Report

    SciTech Connect

    J. E. Francfort; D. V. O'Hara; L. A. Slezak

    1999-05-01

    The Field Operations Program is an electric vehicle testing and evaluation program sponsored by US Department of Energy and managed by the Idaho National Engineering and Environmental Laboratory. The Program's goals are to evaluate electric vehicles in real-world applications and environments, support electric vehicle technology advancement, develop infrastructure elements necessary to support significant electric vehicle use, support increased use of electric vehicles in federal fleets, and increase overall awareness and acceptance of electric vehicles. This report covers Program activities from fiscal year 1997 through mid-fiscal year 1999. The Field Operations Program succeeded the Site Operator Program, which ended in September 1996. Electric vehicle testing conducted by the Program includes baseline performance testing (EV America testing), accelerated reliability (life-cycle) testing, and fleet testing. The baseline performance parameters include accelerations, braking, range, energy efficiency, and charging time. The Program collects accelerated reliability and fleet operations data on electric vehicles operated by the Program's Qualified Vehicle Testing (QVT) partners. The Program's QVT partners have over 3 million miles of electric vehicle operating experience.

  7. Marine and estuarine protection: Programs and activities

    SciTech Connect

    Not Available

    1989-02-01

    The booklet describes: the mission of the current problems and threats to the coastal and marine waters of the US; the Office of Marine and Estuarine Protection of EPA; EPA's ocean dumping and plastics programs; EPA's point source control activities; near-coastal waters activities; and associated federal legislation.

  8. School Programs to Increase Physical Activity

    ERIC Educational Resources Information Center

    Lee, Amelia; Solmon, Melinda

    2007-01-01

    A quality physical education program is at the heart of any plan to promote lifelong participation in physical activity, but it has become evident at many schools that physical education specialists alone cannot address the physical activity needs of children. This is why a series of studies were conducted to develop strategies for the…

  9. Comprehensive School Physical Activity Programs. Position Statement

    ERIC Educational Resources Information Center

    National Association for Sport and Physical Education, 2008

    2008-01-01

    The National Association for Sport and Physical Education (NASPE) recommends that all PK-12 schools implement a Comprehensive School Physical Activity Program. Schools play an important role in public health, and the physical, mental, and social benefits of regular physical activity for youth are well documented. Leading public health, medical,…

  10. Comprehensive School-Based Physical Activity Program

    ERIC Educational Resources Information Center

    Heidorn, Brent D.; Hall, Tina J.; Carson, Russell L.

    2010-01-01

    A Comprehensive School-based Physical Activity Program (CSPAP) represents a commitment to support the health and well-being of students, faculty, staff, and the community. A CSPAP is a similar approach that specifically focuses on incorporating additional physical activity opportunities for youth within the school day and beyond physical education…

  11. [Afterschool physical activity programs: Literature review].

    PubMed

    Reloba-Martínez, Sergio; Martín-Tamayo, Ignacio; Martínez-López, Emilio José; Guerrero-Almeida, Laura

    2015-01-01

    The purpose of this review was to analyze the scientific production about extra-curricular physical activity (PA) in western children of 6-12 years. Medline / Pub-Med, Scopus and Google Scholar were used. This search collects articles published between January 1990 and May 2013. A total of 104 publications were analyzed. The body composition parameters are best used to assess the results of the studies, followed by those which estimate the maximum aerobic capacity. Articles of intervention are presented with very heterogeneous methodological features but there are clear trends in the use of certain aspects. As for the reviews, most are systematic and include meta-analysis. In this studies, body mass index (BMI) is the most used parameter.

  12. Acute stroke therapy with tissue plasminogen activator (tPA) since it was approved by the U.S. Food and Drug Administration (FDA).

    PubMed

    Zivin, Justin A

    2009-07-01

    Tissue plasminogen activator (tPA) for acute ischemic stroke was approved by the U.S. Food and Drug Administration (FDA) in 1996. Since then it has been severely underutilized. At the time when most practitioners were first being exposed to the literature concerning tPA, there were many concerns about safety and the restrictions on use were quite onerous. Since then a good deal of further work has been done to loosen the restrictions and allay concerns about the risks. The true risk to benefit ratio is far better than is generally realized. Now it is mostly economic problems related to the costs of constantly supplying emergency care that is limiting access. Furthermore, in the current litigious environment, failure to treat is likely to be a more hazardous course of action than legal exposure due to poor outcomes. It must be emphasized that the drug is quite safe and highly effective, and current utilization rates are unacceptably low. Ann Neurol 2009;66:6-10.

  13. Oxidation of gas-phase protactinium ions, Pa+ and Pa2+: formation and properties of PaO2(2+)(g), protactinyl.

    PubMed

    Santos, Marta; de Matos, António Pires; Marçalo, Joaquim; Gibson, John K; Haire, Richard G; Tyagi, Rajni; Pitzer, Russell M

    2006-05-04

    Oxidation reactions of bare and ligated, monopositive, and dipositive Pa ions in the gas phase were studied by Fourier transform ion cyclotron resonance mass spectrometry. Seven oxidants were employed, ranging from the thermodynamically robust N(2)O to the relatively weak CH(2)O-all oxidized Pa(+) to PaO(+) and PaO(+) to PaO(2)(+). On the basis of experimental observations, it was established that D[Pa(+)-O] and D[OPa(+)-O] > or = 751 kJ mol(-1). Estimates for D[Pa(+)-O], D[OPa(+)-O], IE[PaO], and IE[PaO(2)] were also obtained. The seven oxidants reacted with Pa(2+) to produce PaO(2+), indicating that D[Pa(2+)-O] > or = 751 kJ mol(-1). A particularly notable finding was the oxidation of PaO(2+) by N(2)O to PaO(2)(2+), a species, which formally comprises Pa(VI). Collision-induced dissociation of PaO(2)(2+) suggested the protactinyl connectivity, {O-Pa-O}(2+). The experimentally determined IE[PaO(2)(+)] approximately 16.6 eV is in agreement with self-consistent-field and configuration interaction calculations for PaO(2)(+) and PaO(2)(2+). These calculations provide insights into the electronic structures of these ions and indicate the participation of 5f orbitals in bonding and a partial "6p hole" in the case of protactinyl. It was found that PaO(2)(2+) catalyzes the oxidation of CO by N(2)O-such O atom transport via a dipositive metal oxide ion is distinctive. It was also observed that PaO(2)(2+) is capable of activating H(2) to form the stable PaO(2)H(2+) ion.

  14. Making Healthy Eating and Physical Activity Policy Practice: Process Evaluation of a Group Randomized Controlled Intervention in Afterschool Programs

    ERIC Educational Resources Information Center

    Weaver, R. Glenn; Beets, Michael W.; Hutto, Brent; Saunders, Ruth P.; Moore, Justin B.; Turner-McGrievy, Gabrielle; Huberty, Jennifer L.; Ward, Dianne S.; Pate, Russell R.; Beighle, Aaron; Freedman, Darcy

    2015-01-01

    This study describes the link between level of implementation and outcomes from an intervention to increase afterschool programs' (ASPs) achievement of healthy eating and physical activity (HE-PA) Standards. Ten intervention ASPs implemented the Strategies-To-Enhance-Practice (STEPs), a multi-component, adaptive intervention framework identifying…

  15. National Dam Inspection Program. Locklin Pond Dam (NDI ID Number PA-00139, DER ID Number 64-31), Delaware River Basin, Lakeville Creek, Wayne County, Pennsylvania. Phase I Inspection Report.

    DTIC Science & Technology

    1980-07-01

    from a vertical mortared- rockfill wall to a nearly-flat earthen slope. The upstream slope is nearly vertical above normal pool along this part of the dam ...history of the dam . In 1917, the dam was described as being constructed of stone and earth fill. At this time a 150-foot long embankment existed...PENNSYLVANIA NDI ID NO. PA-00139 DER ID NO. 64.31 CLIFTON AND LEWIS LOCKLIN PHASE I INSPECTION REPORT NATIONAL DAM INSPECTION PROGRAM Preae by GANNETT

  16. Discovering Community: Activities for Afterschool Programs

    ERIC Educational Resources Information Center

    National Institute on Out-of-School Time, Wellesley College, 2006

    2006-01-01

    The project activities highlighted in this publication were conducted within the framework of school-based afterschool programs operated by community-based organizations. The intention of the Discovering Community initiative, created by The After-School Corporation and MetLife Foundation, is to foster greater collaborations and mutual respect…

  17. A combination of desmopressin and docetaxel inhibit cell proliferation and invasion mediated by urokinase-type plasminogen activator (uPA) in human prostate cancer cells

    SciTech Connect

    Sasaki, Hiroshi; Klotz, Laurence H.; Sugar, Linda M.; Kiss, Alexander; Venkateswaran, Vasundara

    2015-08-28

    Background: This study was designed to assess the effectiveness of a combination treatment using both desmopressin and docetaxel in prostate cancer treatment. Desmopressin is a well-known synthetic analogue of the antidiuretic hormone vasopressin. It has recently been demonstrated to inhibit tumor progression and metastasis in in vivo models. Docetaxel is widely used for the treatment of castration resistant prostate cancer (CRPC) patients. However, durable responses have been uncommon to date. In this study, we investigated the anti-tumor effect of desmopressin in combination with docetaxel in vitro and in vivo. Methods: Two prostate cancer cells (PC3, LNCaP) were treated with different concentrations of desmopressin alone, docetaxel alone, and a combination of desmopressin and docetaxel. Cell proliferation was determined by MTS assay. The anti-invasive and anti-migration potential of desmopressin and in combination with docetaxel were examined by wound healing assay, migration chamber assay, and matrigel invasion assay. Results: The combination of desmopressin and docetaxel resulted in a significant inhibition of PC3 and LNCaP cell proliferation (p < 0.01). Additionally, cell migration and invasion were also inhibited by the combination when compared to that of either treatment alone in PC3 cells (p < 0.01). The anti-tumor effect of this combination treatment was associated with down-regulation of both urokinase-type plasminogen activator (uPA) and matrix metalloproteinase (MMP-2 and MMP-9) in PC3 cells. Conclusions: We are the first to elucidate the anti-tumor and anti-metastatic potential of desmopressin in combination with docetaxel in a prostate cancer model via the uPA-MMP pathway. Our finding could potentially contribute to the therapeutic profile of desmopressin and enhance the efficacy of docetaxel based treatment for CRPC. - Highlights: • Desmopressin inhibits cell proliferation in prostate cancer cells. • The expression of cyclin A and CDK2

  18. 10 CFR 1042.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Education programs or activities. 1042.400 Section 1042... EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 1042.400 Education programs or activities....

  19. 10 CFR 1042.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Education programs or activities. 1042.400 Section 1042... EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 1042.400 Education programs or activities....

  20. 10 CFR 1042.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Education programs or activities. 1042.400 Section 1042... EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 1042.400 Education programs or activities....

  1. 10 CFR 1042.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Education programs or activities. 1042.400 Section 1042... EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 1042.400 Education programs or activities....

  2. Reduction in ATP levels triggers immunoproteasome activation by the 11S (PA28) regulator during early antiviral response mediated by IFNβ in mouse pancreatic β-cells.

    PubMed

    Freudenburg, Wieke; Gautam, Madhav; Chakraborty, Pradipta; James, Jared; Richards, Jennifer; Salvatori, Alison S; Baldwin, Aaron; Schriewer, Jill; Buller, R Mark L; Corbett, John A; Skowyra, Dorota

    2013-01-01

    Autoimmune destruction of insulin producing pancreatic β-cells is the hallmark of type I diabetes. One of the key molecules implicated in the disease onset is the immunoproteasome, a protease with multiple proteolytic sites that collaborates with the constitutive 19S and the inducible 11S (PA28) activators to produce immunogenic peptides for presentation by MHC class I molecules. Despite its importance, little is known about the function and regulation of the immunoproteasome in pancreatic β-cells. Of special interest to immunoproteasome activation in β-cells are the effects of IFNβ, a type I IFN secreted by virus-infected cells and implicated in type I diabetes onset, compared to IFNγ, the classic immunoproteasome inducer secreted by cells of the immune system. By qPCR analysis, we show that mouse insulinoma MIN6 cells and mouse islets accumulate the immune proteolytic β1(i), β2(i) and β5(i), and 11S mRNAs upon exposure to IFNβ or IFNγ. Higher concentrations of IFNβ than IFNγ are needed for similar expression, but in each case the expression is transient, with maximal mRNA accumulation in 12 hours, and depends primarily on Interferon Regulatory Factor 1. IFNs do not alter expression of regular proteasome genes, and in the time frame of IFNβ-mediated response, the immune and regular proteolytic subunits co-exist in the 20S particles. In cell extracts with ATP, these particles have normal peptidase activities and degrade polyubiquitinated proteins with rates typical of the regular proteasome, implicating normal regulation by the 19S activator. However, ATP depletion rapidly stimulates the catalytic rates in a manner consistent with levels of the 11S activator. These findings suggest that stochastic combination of regular and immune proteolytic subunits may increase the probability with which unique immunogenic peptides are produced in pancreatic β-cells exposed to IFNβ, but primarily in cells with reduced ATP levels that stimulate the 11S

  3. Rates and determinants of uptake and use of an internet physical activity and weight management program in office and manufacturing work sites in England: Cohort Study

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Internet-based physical activity (PA) and weight management programs have the potential to improve employees' health in large occupational health settings. To be successful, the program must engage a wide range of employees, especially those at risk of weight gain or ill health. The aim of the study...

  4. School-Age Ideas and Activities for After School Programs.

    ERIC Educational Resources Information Center

    Haas-Foletta, Karen; Cogley, Michele

    This guide describes activities for school-age children in after-school day care programs. These activities may also be used in other settings. An introductory section discusses program philosophy, room arrangement, multicultural curriculum, program scheduling, summer programs and holiday care, field trips and special programs, age grouping,…

  5. Tumour-associated urokinase-type plasminogen activator (uPA) and its inhibitor PAI-1 in normal and neoplastic tissues of patients with squamous cell cancer of the oral cavity - clinical relevance and prognostic value.

    PubMed

    Hundsdorfer, Brigitte; Zeilhofer, Hans-Florian; Bock, Klaus Peter; Dettmar, Peer; Schmitt, Manfred; Kolk, Andreas; Pautke, Christoph; Horch, Hans-Henning

    2005-06-01

    The central role of the serine protease urokinase-type plasminogen activator (uPA) and its inhibitor, the plasminogen activator-inhibitor-1 (PAI-1), in tumour invasion and metastasis becomes more and more evident. In several studies, uPA and PAI-1 proved to be of prognostic relevance as shown for different types of cancer (e.g. breast, stomach, lung). Elevated antigen levels of uPA and/or PAI-1 predict poor outcome (relapse-free survival) for patients afflicted with cancer. For oral squamous cell carcinomas, however, the prognostic relevance of the tumour-associated proteolytic factors uPA and PAI-1 has still to be evaluated. In the present study, using tissue extracts of 79 oral cancer cases, 58 specimens of normal oral cavity mucosa and of 16 tumour positive lymph nodes taken from the same patients, uPA and PAI-1 antigen were determined by highly sensitive enzyme-linked immunosorbent assays (ELISA). A correlation was found between uPA and PAI-1 in tumour tissue, when compared with the normal mucosa of the same oral cavity. Median levels showed significant elevations in cancer tissue and in tumour positive lymph nodes versus normal oral mucosa. In patients with high levels of uPA or PAI-1, there were significantly more tumour relapses. There was no significant correlation between pathological TNM categories, grading, residual tumour category, tumour site and patient age. In summary, tumour uPA/PAI-1 content (as determined by ELISA) appears to be a strong independent prognostic factor for relapse-free survival in squamous cell cancer of the oral cavity. These observations might help to select patients with poor prognosis for additional adjuvant therapy in conjunction with complete surgical resection.

  6. Active Sites Environmental Monitoring Program: Program plan. Revision 1

    SciTech Connect

    Ashwood, T.L.; Wickliff, D.S.; Morrissey, C.M.

    1992-02-01

    The Active Sites Environmental Monitoring Program (ASEMP), initiated in 1989, provides early detection and performance monitoring of transuranic (TRU) waste and active low-level waste (LLW) facilities at Oak Ridge National Laboratory (ORNL) in accordance with US Department of Energy (DOE) Order 5820.2A. Active LLW facilities in Solid Waste Storage Area (SWSA) 6 include Tumulus I and Tumulus II, the Interim Waste Management Facility (IWMF), LLW silos, high-range wells, asbestos silos, and fissile wells. The tumulus pads and IWMF are aboveground, high-strength concrete pads on which concrete vaults containing metal boxes of LLW are placed; the void space between the boxes and vaults is filled with grout. Eventually, these pads and vaults will be covered by an engineered multilayered cap. All other LLW facilities in SWSA 6 are below ground. In addition, this plan includes monitoring of the Hillcut Disposal Test Facility (HDTF) in SWSA 6, even though this facility was completed prior to the data of the DOE order. In SWSA 5 North, the TRU facilities include below-grade engineered caves, high-range wells, and unlined trenches. All samples from SWSA 6 are screened for alpha and beta activity, counted for gamma-emitting isotopes, and analyzed for tritium. In addition to these analytes, samples from SWSA 5 North are analyzed for specific transuranic elements.

  7. 31 CFR 28.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 31 Money and Finance: Treasury 1 2012-07-01 2012-07-01 false Education programs or activities. 28... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 28.400 Education programs...

  8. 22 CFR 146.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Education programs or activities. 146.400... IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 146.400 Education programs or...

  9. 28 CFR 54.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Education programs or activities. 54.400... SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 54.400 Education programs or...

  10. 6 CFR 17.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 6 Domestic Security 1 2013-01-01 2013-01-01 false Education programs or activities. 17.400 Section... ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 17.400 Education programs...

  11. 31 CFR 28.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 31 Money and Finance: Treasury 1 2014-07-01 2014-07-01 false Education programs or activities. 28... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 28.400 Education programs...

  12. 45 CFR 618.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 3 2013-10-01 2013-10-01 false Education programs or activities. 618.400 Section... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 618.400 Education programs...

  13. 6 CFR 17.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 6 Domestic Security 1 2012-01-01 2012-01-01 false Education programs or activities. 17.400 Section... ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 17.400 Education programs...

  14. 15 CFR 8a.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Education programs or activities. 8a... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.400 Education programs...

  15. 45 CFR 86.31 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 1 2013-10-01 2013-10-01 false Education programs or activities. 86.31 Section 86... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 86.31 Education programs...

  16. 15 CFR 8a.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Education programs or activities. 8a... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.400 Education programs...

  17. 43 CFR 41.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Education programs or activities. 41.400... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 41.400 Education programs...

  18. 43 CFR 41.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 43 Public Lands: Interior 1 2013-10-01 2013-10-01 false Education programs or activities. 41.400... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 41.400 Education programs...

  19. 28 CFR 54.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Education programs or activities. 54.400... SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 54.400 Education programs or...

  20. 41 CFR 101-4.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 41 Public Contracts and Property Management 2 2014-07-01 2012-07-01 true Education programs or... EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 101-4.400 Education programs or activities....

  1. 6 CFR 17.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 6 Domestic Security 1 2014-01-01 2014-01-01 false Education programs or activities. 17.400 Section... ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 17.400 Education programs...

  2. 45 CFR 86.31 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 1 2011-10-01 2011-10-01 false Education programs or activities. 86.31 Section 86... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 86.31 Education programs...

  3. 6 CFR 17.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 6 Domestic Security 1 2010-01-01 2010-01-01 false Education programs or activities. 17.400 Section... ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 17.400 Education programs...

  4. 41 CFR 101-4.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 41 Public Contracts and Property Management 2 2013-07-01 2012-07-01 true Education programs or... EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 101-4.400 Education programs or activities....

  5. 15 CFR 8a.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Education programs or activities. 8a... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.400 Education programs...

  6. 22 CFR 146.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Education programs or activities. 146.400... IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 146.400 Education programs or...

  7. 49 CFR 25.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 1 2014-10-01 2014-10-01 false Education programs or activities. 25.400 Section... IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 25.400 Education programs or...

  8. 31 CFR 28.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 31 Money and Finance: Treasury 1 2013-07-01 2013-07-01 false Education programs or activities. 28... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 28.400 Education programs...

  9. 40 CFR 5.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 1 2012-07-01 2012-07-01 false Education programs or activities. 5.400... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 5.400 Education programs...

  10. 45 CFR 618.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 3 2012-10-01 2012-10-01 false Education programs or activities. 618.400 Section... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 618.400 Education programs...

  11. 6 CFR 17.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 6 Domestic Security 1 2011-01-01 2011-01-01 false Education programs or activities. 17.400 Section... ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 17.400 Education programs...

  12. 22 CFR 229.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Education programs or activities. 229.400... SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 229.400 Education programs or...

  13. 41 CFR 101-4.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 2 2010-07-01 2010-07-01 true Education programs or... EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 101-4.400 Education programs or activities....

  14. 49 CFR 25.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 1 2011-10-01 2011-10-01 false Education programs or activities. 25.400 Section... IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 25.400 Education programs or...

  15. 40 CFR 5.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 1 2013-07-01 2013-07-01 false Education programs or activities. 5.400... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 5.400 Education programs...

  16. 40 CFR 5.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 1 2011-07-01 2011-07-01 false Education programs or activities. 5.400... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 5.400 Education programs...

  17. 28 CFR 54.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Education programs or activities. 54.400... SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 54.400 Education programs or...

  18. 49 CFR 25.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 1 2012-10-01 2012-10-01 false Education programs or activities. 25.400 Section... IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 25.400 Education programs or...

  19. 45 CFR 618.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Education programs or activities. 618.400 Section... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 618.400 Education programs...

  20. 41 CFR 101-4.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 41 Public Contracts and Property Management 2 2012-07-01 2012-07-01 false Education programs or... EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 101-4.400 Education programs or activities....

  1. 45 CFR 86.31 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 1 2014-10-01 2014-10-01 false Education programs or activities. 86.31 Section 86... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 86.31 Education programs...

  2. 49 CFR 25.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Education programs or activities. 25.400 Section... IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 25.400 Education programs or...

  3. 43 CFR 41.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 43 Public Lands: Interior 1 2012-10-01 2011-10-01 true Education programs or activities. 41.400... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 41.400 Education programs...

  4. 22 CFR 229.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Education programs or activities. 229.400... SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 229.400 Education programs or...

  5. 31 CFR 28.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 31 Money and Finance: Treasury 1 2011-07-01 2011-07-01 false Education programs or activities. 28... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 28.400 Education programs...

  6. 31 CFR 28.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Education programs or activities. 28... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 28.400 Education programs...

  7. 43 CFR 41.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 Public Lands: Interior 1 2011-10-01 2011-10-01 false Education programs or activities. 41.400... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 41.400 Education programs...

  8. 22 CFR 146.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Education programs or activities. 146.400... IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 146.400 Education programs or...

  9. 28 CFR 54.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Education programs or activities. 54.400... SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 54.400 Education programs or...

  10. 15 CFR 8a.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Education programs or activities. 8a... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.400 Education programs...

  11. 41 CFR 101-4.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 41 Public Contracts and Property Management 2 2011-07-01 2007-07-01 true Education programs or... EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 101-4.400 Education programs or activities....

  12. 22 CFR 229.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Education programs or activities. 229.400... SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 229.400 Education programs or...

  13. 40 CFR 5.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 1 2014-07-01 2014-07-01 false Education programs or activities. 5.400... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 5.400 Education programs...

  14. 15 CFR 8a.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Education programs or activities. 8a... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.400 Education programs...

  15. 22 CFR 229.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Education programs or activities. 229.400... SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 229.400 Education programs or...

  16. 22 CFR 229.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Education programs or activities. 229.400... SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 229.400 Education programs or...

  17. 45 CFR 618.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 3 2011-10-01 2011-10-01 false Education programs or activities. 618.400 Section... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 618.400 Education programs...

  18. 22 CFR 146.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Education programs or activities. 146.400... IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 146.400 Education programs or...

  19. Degradation of Internalized αvβ5 Integrin Is Controlled by uPAR Bound uPA: Effect on β1 Integrin Activity and α-SMA Stress Fiber Assembly

    PubMed Central

    Wang, Lingyan; Pedroja, Benjamin S.; Meyers, Erin E.; Garcia, Angelo L.; Twining, Sally S.; Bernstein, Audrey M.

    2012-01-01

    Myofibroblasts (Mfs) that persist in a healing wound promote extracellular matrix (ECM) accumulation and excessive tissue contraction. Increased levels of integrin αvβ5 promote the Mf phenotype and other fibrotic markers. Previously we reported that maintaining uPA (urokinase plasminogen activator) bound to its cell-surface receptor, uPAR prevented TGFβ-induced Mf differentiation. We now demonstrate that uPA/uPAR controls integrin β5 protein levels and in turn, the Mf phenotype. When cell-surface uPA was increased, integrin β5 levels were reduced (61%). In contrast, when uPA/uPAR was silenced, integrin β5 total and cell-surface levels were increased (2–4 fold). Integrin β5 accumulation resulted from a significant decrease in β5 ubiquitination leading to a decrease in the degradation rate of internalized β5. uPA-silencing also induced α-SMA stress fiber organization in cells that were seeded on collagen, increased cell area (1.7 fold), and increased integrin β1 binding to the collagen matrix, with reduced activation of β1. Elevated cell-surface integrin β5 was necessary for these changes after uPA-silencing since blocking αvβ5 function reversed these effects. Our data support a novel mechanism by which downregulation of uPA/uPAR results in increased integrin αvβ5 cell-surface protein levels that regulate the activity of β1 integrins, promoting characteristics of the persistent Mf. PMID:22470492

  20. Combined Diet and Physical Activity Promotion Programs to Prevent Type 2 Diabetes Among People at Increased Risk: A Systematic Review for the Community Preventive Services Task Force

    PubMed Central

    Balk, Ethan M.; Earley, Amy; Raman, Gowri; Avendano, Esther A.; Pittas, Anastassios G.; Remington, Patrick L.

    2015-01-01

    Background Trials have demonstrated the efficacy of rigorous diet and physical activity promotion (D&PA) programs for adults at increased risk for type 2 diabetes to reduce diabetes incidence and improve measures of glycemia. Purpose To evaluate D&PA programs for individuals at increased risk for type 2 diabetes primarily to lower diabetes risk, lower body weight, and improve glycemia. Data Sources MEDLINE, Cochrane Central Register of Controlled Trials, CAB Abstracts, Global Health, and Ovid HealthStar from 1991 through 27 February 2015, with no language restriction. Study Selection 8 researchers screened articles for single group or comparative studies of combined D&PA programs with at least 2 sessions of at least 3 month duration in participants at increased risk for type 2 diabetes. Data Extraction 7 researchers extracted data—on study design, participant, intervention, outcome descriptions, and results—and assessed study quality. Data Synthesis 53 studies (30 D&PA vs. control, 13 more vs. less intensive, 13 in single programs) evaluated 66 programs. Compared with usual care, D&PA reduced type 2 diabetes incidence (RR = 0.59; 95% CI 0.51, 0.66; 16 studies), lowered body weight (net change = −2.2%; 95% CI −2.9, −1.4; 24 studies) and fasting blood glucose (net change = −0.12 mmol/L; 95% CI −0.20, −0.05; 17 studies), and improved other cardiometabolic risk factors. There was limited evidence for clinical events. More intensive programs were more effective. Limitations The wide variation in D&PA programs limited identification of features most relevant to effectiveness. Evidence on clinical outcomes and in children was sparse. Conclusions Combined D&PA promotion programs are effective to decrease diabetes incidence and improve cardiometabolic risk factors for patients at increased risk. More intensive programs are more effective. Primary Funding Source Centers for Disease Control and Prevention Community Preventive Services Task Force. PMID:26167912

  1. Space activity and programs at Sofradir

    NASA Astrophysics Data System (ADS)

    Bouakka-Manesse, A.; Jamin, N.; Delannoy, A.; Fièque, B.; Leroy, C.; Pidancier, P.; Vial, L.; Chorier, P.; Péré Laperne, N.

    2016-10-01

    SOFRADIR is one of the leading companies involved in the development and manufacturing of infrared detectors for space applications. As a matter of fact, SOFRADIR is involved in many space programs from visible up to VLWIR spectral ranges. These programs concern operational missions for earth imagery, meteorology and also scientific missions for universe exploration. One of the last space detectors available at SOFRADIR is a visible - SWIR detector named Next Generation Panchromatic Detector (NGP) which is well adapted for hyperspectral, imagery and spectroscopy applications. In parallel of this new space detector, numerous programs are currently running for different kind of missions: meteorology (MTG), Copernicus with the Sentinel detectors series, Metop-SG system (3MI), Mars exploration (Mamiss, etc….)… In this paper, we present the last developments made for space activity and in particular the NGP detector. We will also present the space applications using this detector and show appropriateness of its use to answer space programs specifications, as for example those of Sentinel-5.

  2. 32 CFR 701.123 - PA fees.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... duplication for free. (1) DON activities shall waive fees automatically if the direct cost for reproduction of... made on a case-to-case basis. (c) PA fee deposits. Checks or money orders shall be made payable to...

  3. A higher body temperature is associated with haemorrhagic transformation in patients with acute stroke untreated with recombinant tissue-type plasminogen activator (rtPA).

    PubMed

    Leira, Rogelio; Sobrino, Tomás; Blanco, Miguel; Campos, Francisco; Rodríguez-Yáñez, Manuel; Castellanos, Mar; Moldes, Octavio; Millán, Mónica; Dávalos, Antoni; Castillo, José

    2012-02-01

    Higher body temperature is a prognostic factor of poor outcome in acute stroke. Our aim was to study the relationship between body temperature, HT (haemorrhagic transformation) and biomarkers of BBB (blood-brain barrier) damage in patients with acute ischaemic stroke untreated with rtPA (recombinant tissue-type plasminogen activator). We studied 229 patients with ischaemic stroke <12 h from symptom onset. Body temperature was determined at admission and every 6 h during the first 3 days. HT was evaluated according to ECASS II (second European Co-operative Acute Stroke Study) criteria in a multimodal MRI (magnetic resonance imaging) at 72 h. We found that 55 patients (34.1%) showed HT. HT was associated with cardioembolic stroke (64.2% against 23.0%; P<0.0001), higher body temperature during the first 24 h (36.9°C compared with 36.5°C; P<0.0001), more severe stroke [NIHSS (National Institutes of Health Stroke Scale) score, 14 (9-20) against 10 (7-15); P=0.002], and greater DWI (diffusion-weighted imaging) lesion volume at admission (23.2 cc compared with 13.2 cc; P<0.0001). Plasma MMP-9 (matrix metalloproteinase 9) (187.3 ng/ml compared with 44.2 ng/ml; P<0.0001) and cFn (cellular fibronectin) levels (16.3 μg/ml compared with 7.1 μg/ml; P=0.001) were higher in patients with HT. Body temperature within the first 24 h was independently associated with HT {OR (odds ratio), 7.3 [95% CI (confidence interval), 2.4-22.6]; P<0.0001} after adjustment for cardioembolic stroke subtype, baseline NIHSS score and DWI lesion volume. This effect remained unchanged after controlling for MMP-9 and cFn. In conclusion, high body temperature within the first 24 h after ischaemic stroke is a risk factor for HT in patients untreated with rtPA. This effect is independent of some biological signatures of BBB damage.

  4. PA Discussion Topics

    DTIC Science & Technology

    2011-02-04

    Vehicle Power & Mobility PA Discussion Topics UNCLASSIFIED: Dist A. Approved for public releas Report Documentation Page Form ApprovedOMB No. 0704...0188 Public reporting burden for the collection of information is estimated to average 1 hour per response, including the time for reviewing instructions...Headquarters Services, Directorate for Information Operations and Reports , 1215 Jefferson Davis Highway, Suite 1204, Arlington VA 22202-4302

  5. National Dam Inspection Program. Butler’s Lake Dam (NDI Number PA-01067, PennDER Number 2-29) Ohio River Basin, Drennen Branch, Allegheny County, Pennsylvania. Phase I Inspection Report.

    DTIC Science & Technology

    1981-05-01

    PENNSYLVANIA 15216 MAY 1981 81 7 0 o 7 OHIO RIVER BASIN BUTLER’S LAKE DAM ALLEGHENY COUNTY, COMMONWEALTH OF PENNSYLVANIA NDI NO. PA 01067 PennDER NO. 2...been affected by small country banks. F1 )1’u nt Vemnur FPm Pmm Bun Vs PPm PIpw pm ’ c-AMST f ~.Indt / t0s. 3 0 -(Fran PPw PmPm c/ 13 vthedale...STRUCTURE CONTOUR MAP1975 DATE: MAY 1981 BUTLER’S LAKE DAM SCALE: 1"-:2000’ NATIONAL DAM INSPECTION PROGRAM I GEOLOGIC DR: JF ICK: j ACKENI4EIL & ASSOCIATES

  6. National Dam Inspection Program. Macham Dam (NDI I.D. Number PA-00043 Pennder I.D. Number 8-56) Susquehanna River Basin. Wolcott Creek, Bradford County, Pennsylvania. Phase I Inspection Report,

    DTIC Science & Technology

    1980-07-01

    INSPECTION PROGRAM ABSTRACT Macham Dam: NDI I.D. No. PA-00043 Owner: Manley and Afton Chamberlain State Located: Pennsylvania (PennDER I.D. No. 8-56...storage capacity at top of dam. d. Hazard Classification. High (see Section 3.l.e). e. Ownership. Manley and Afton Chamberlain Box 122 R.D. 2 Wellsburg...N.Y. 14894 1 f. Purpose. Recreation. h. Historical Data. Macham Dam is owned by Manley and Afton Chamberlain, a father and son partnership who conceived

  7. Short- and Long-Term Theory-Based Predictors of Physical Activity in Women Who Participated in a Weight-Management Program

    ERIC Educational Resources Information Center

    Wasserkampf, A.; Silva, M. N.; Santos, I. C.; Carraça, E. V.; Meis, J. J. M.; Kremers, S. P. J.; Teixeira, P. J.

    2014-01-01

    This study analyzed psychosocial predictors of the Theory of Planned Behavior (TPB) and Self-Determination Theory (SDT) and evaluated their associations with short- and long-term moderate plus vigorous physical activity (MVPA) and lifestyle physical activity (PA) outcomes in women who underwent a weight-management program. 221 participants (age…

  8. Playing by Programming: Making Gameplay a Programming Activity

    ERIC Educational Resources Information Center

    Weintrop, David; Wilensky, Uri

    2016-01-01

    Video games are an oft-cited reason for young learners getting interested in programming and computer science. As such, many learning opportunities build on this interest by having kids program their own video games. This approach, while sometimes successful, has its drawbacks stemming from the fact that the challenge of programming and game…

  9. National childhood diabetes program activities in Turkey.

    PubMed

    Hatun, Şükrü

    2015-03-01

    Recent census figures in Turkey show that out of a population of 76.6 million, 22.7 million (29.7%) are younger than 18 years old. The great majority (>95%) of pediatric cases of diabetes in Turkey are type 1 diabetes mellitus (T1DM). In recent years, with increase in number of pediatric endocrine centers around the country, the important issue of care for diabetic children and adolescents has been revived and major steps have been taken for improvement in pediatric care and its outreach to all diabetic children. The Childhood Diabetes Group continues its activities in cooperation with the Turkish Ministry of Health. A list of areas of interest of the Group include "School programs", "Incidence/prevalence studies and national registry system", "Educational guidelines for diabetes in children", "Increasing the numbers of camps and summer schools for diabetic children", "Organization of educational programs for the health team", "National guidelines for transition of diabetic children to adult clinics", "Improvement of school canteens", "Educational spots" to improve awareness of diabetes. The activities of the Childhood Diabetes Group will be discussed in detail in this article.

  10. After-School Physical Activity Programs for Adolescent Girls.

    ERIC Educational Resources Information Center

    Watson, Doris L.; Poczwaradowski, Artur; Eisenman, Pat

    2000-01-01

    Describes adolescent girls' responses to an after-school physical activity program, examining how it functioned as a listening tool within a social marketing approach to promoting physical activity. Focus groups and interviews indicated that girls enjoyed and valued the program. Though the program did not increase girls' physical activity levels,…

  11. Trends toward the Future in Physical Activity Programming

    ERIC Educational Resources Information Center

    Charles, John M.; Charles, P. Kelly

    2016-01-01

    In the wake of multiple activity program eliminations and frequent downgrading of activity programs nationwide, the question to be addressed is how we can draw upon our interdisciplinary, intergenerational, and intercultural understandings of human movement to construct physical activity programs for the future that might withstand the test of…

  12. 28 CFR 54.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 54.400 Education programs or activities... basis of sex, be excluded from participation in, be denied the benefits of, or be subjected...

  13. 45 CFR 86.31 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 86.31 Education programs or activities. (a) General. Except as provided elsewhere in this part, no person shall, on the basis of sex,...

  14. 45 CFR 86.31 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 86.31 Education programs or activities. (a) General. Except as provided elsewhere in this part, no person shall, on the basis of sex,...

  15. 14 CFR 1253.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 5 2013-01-01 2013-01-01 false Education programs or activities. 1253.400... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 1253.400 Education...

  16. 13 CFR 113.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Education programs or activities... ADMINISTRATOR Nondiscrimination on the Basis of Sex in Education Programs or Activities Receiving Federal Financial Assistance Discrimination on the Basis of Sex in Education Programs Or Activities Prohibited §...

  17. 13 CFR 113.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Education programs or activities... ADMINISTRATOR Nondiscrimination on the Basis of Sex in Education Programs or Activities Receiving Federal Financial Assistance Discrimination on the Basis of Sex in Education Programs Or Activities Prohibited §...

  18. 13 CFR 113.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Education programs or activities... ADMINISTRATOR Nondiscrimination on the Basis of Sex in Education Programs or Activities Receiving Federal Financial Assistance Discrimination on the Basis of Sex in Education Programs Or Activities Prohibited §...

  19. 13 CFR 113.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Education programs or activities... ADMINISTRATOR Nondiscrimination on the Basis of Sex in Education Programs or Activities Receiving Federal Financial Assistance Discrimination on the Basis of Sex in Education Programs Or Activities Prohibited §...

  20. 14 CFR 1253.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 5 2011-01-01 2010-01-01 true Education programs or activities. 1253.400... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 1253.400 Education...

  1. Functional Characterization of Phalaenopsis aphrodite Flowering Genes PaFT1 and PaFD.

    PubMed

    Jang, Seonghoe; Choi, Sang-Chul; Li, Hsing-Yi; An, Gynheung; Schmelzer, Elmon

    2015-01-01

    We show that the key flowering regulators encoded by Phalaenopsis aphrodite FLOWERING LOCUS T1 (PaFT1) and PaFD share high sequence homologies to these from long-day flowering Arabidopsis and short-day flowering rice. Interestingly, PaFT1 is specifically up-regulated during flowering inductive cooling treatment but is not subjected to control by photoperiod in P. aphrodite. Phloem or shoot apex-specific expression of PaFT1 restores the late flowering of Arabidopsis ft mutants. Moreover, PaFT1 can suppress the delayed flowering caused by SHORT VEGATATIVE PHASE (SVP) overexpression as well as an active FRIGIDA (FRI) allele, indicating the functional conservation of flowering regulatory circuit in different plant species. PaFT1 promoter:GUS in Arabidopsis showed similar staining pattern to that of Arabidopsis FT in the leaves and guard cells but different in the shoot apex. A genomic clone or heat shock-inducible expression of PaFT1 is sufficient to the partial complementation of the ft mutants. Remarkably, ectopic PaFT1 expression also triggers precocious heading in rice. To further demonstrate the functional conservation of the flowering regulators, we show that PaFD, a bZIP transcription factor involved in flowering promotion, interacts with PaFT1, and PaFD partially complemented Arabidopsis fd mutants. Transgenic rice expressing PaFD also flowered early with increased expression of rice homologues of APETALA1 (AP1). Consistently, PaFT1 knock-down Phalaenopsis plants generated by virus-induced gene silencing exhibit delayed spiking. These studies suggest functional conservation of FT and FD genes, which may have evolved and integrated into distinct regulatory circuits in monopodial orchids, Arabidopsis and rice that promote flowering under their own inductive conditions.

  2. Functional Characterization of Phalaenopsis aphrodite Flowering Genes PaFT1 and PaFD

    PubMed Central

    Jang, Seonghoe; Choi, Sang-Chul; Li, Hsing-Yi; An, Gynheung; Schmelzer, Elmon

    2015-01-01

    We show that the key flowering regulators encoded by Phalaenopsis aphrodite FLOWERING LOCUS T1 (PaFT1) and PaFD share high sequence homologies to these from long-day flowering Arabidopsis and short-day flowering rice. Interestingly, PaFT1 is specifically up-regulated during flowering inductive cooling treatment but is not subjected to control by photoperiod in P. aphrodite. Phloem or shoot apex-specific expression of PaFT1 restores the late flowering of Arabidopsis ft mutants. Moreover, PaFT1 can suppress the delayed flowering caused by SHORT VEGATATIVE PHASE (SVP) overexpression as well as an active FRIGIDA (FRI) allele, indicating the functional conservation of flowering regulatory circuit in different plant species. PaFT1 promoter:GUS in Arabidopsis showed similar staining pattern to that of Arabidopsis FT in the leaves and guard cells but different in the shoot apex. A genomic clone or heat shock-inducible expression of PaFT1 is sufficient to the partial complementation of the ft mutants. Remarkably, ectopic PaFT1 expression also triggers precocious heading in rice. To further demonstrate the functional conservation of the flowering regulators, we show that PaFD, a bZIP transcription factor involved in flowering promotion, interacts with PaFT1, and PaFD partially complemented Arabidopsis fd mutants. Transgenic rice expressing PaFD also flowered early with increased expression of rice homologues of APETALA1 (AP1). Consistently, PaFT1 knock-down Phalaenopsis plants generated by virus-induced gene silencing exhibit delayed spiking. These studies suggest functional conservation of FT and FD genes, which may have evolved and integrated into distinct regulatory circuits in monopodial orchids, Arabidopsis and rice that promote flowering under their own inductive conditions. PMID:26317412

  3. Nonproliferation and counterproliferation activities and programs

    SciTech Connect

    1994-05-01

    At least twenty countries-many of them hostile to the United States and its allies-have now or are seeking to develop the capability to produce nuclear, biological and/or chemical weapons of mass destruction and the means to deliver them. More than twelve countries have operational ballistic missiles, and others have programs to develop them. Weapons of mass destruction may directly threaten US forces in the field and, in a more perplexing way, threaten the effective force employment by requiring dispersal of those forces. Potential adversaries may use weapons of mass destruction to deter US power projection abroad. As President Clinton stated to `If we do not stem the proliferation of the world`s deadliest weapons, no democracy can feel secure.` Because of concern over this threat, the National Defense Authorization Act of 1994 (NDAA 94) required the establishment of an interagency review committee composed of representatives from the Departments of State, Defense, Energy, the Intelligence Community, the Joint Chiefs of Staff and the Arms Control Disarmament Agency and tasked the committee to report on nonproliferation and counterproliferation activities and programs. To ensure comprehensiveness, representatives of other departments and agencies were asked to participate. In accordance with NDAA 94, this report provides a top-down overview of existing, planned and proposed capabilities and technologies, as well as a description of priorities, programmatic options and other issues.

  4. Veno-occlusive disease (VOD) of the liver in Korean patients following allogeneic bone marrow transplantation (BMT): efficacy of recombinant human tissue plasminogen activator (rt-PA) treatment.

    PubMed

    Lee, J H; Lee, K H; Choi, J S; Zang, D Y; Kim, S B; Kim, S W; Suh, C; Lee, J S; Kim, W K; Lee, Y S; Kim, S H

    1996-04-01

    Veno-occlusive disease (VOD) of the liver is a clinical syndrome characterized by hyperbilirubinemia, painful hepatomegaly, and fluid retention. In the bone marrow transplantation (BMT) setting, VOD is caused by dose-intensive chemotherapy and/or radiotherapy used to prepare patients for transplant. VOD occurs in up to 50% of the patients who undergo BMT and is usually associated with a high mortality rate. Until recently, there was no proven effective medical therapy for this condition once it was clinically apparent. We report here on the frequency and treatment result of VOD with rt-PA in our allogeneic BMT patients. Eight patients (median age 28.5 years) underwent allogeneic BMT from December, 1993 to June, 1995 in Asan Medical Center. Six leukemia patients were prepared for BMT with busulfan and cyclophosphmide, while two aplastic anemia patients received cyclophosphamide and antithymocyte globulin. VOD was defined as having two of the following features before day 20 posttransplant: jaundice (bilirubin > or = 2 mg/dL), tender hepatomegaly and/or right upper quadrant pain, ascites and/or unexplained weight gain (> 2% from baseline). All patients who were diagnosed with VOD received rt-PA (10-20 mg/day) and heparin (10,000 U/day). Three (37.5%) of the eight patients developed VOD that occurred between 6 and 10 days posttransplant. All three patients developed jaundice, weight gain, and tender hepatomegaly. Ascites and renal insufficiency occurred in two patients and pleural effusion in one patient. rt-PA and heparin were begun 6 to 26 days posttransplant and rt-PA was administered for 7 to 14 days. All three patients responded to the therapy; bilirubin levels began to decrease at 4 to 13 days from the start of therapy. They are all alive at day 111, 316, and 548 days posttransplant. None of the patients had significant hemorrhagic complications after rt-PA treatment. Prolonged administration of rt-PA was feasible without bleeding episode and it seems that rt-PA

  5. RE/SPEC Inc. technical support to the Repository Technology Program; Summary of activities for September 1, 1988--June 30, 1992

    SciTech Connect

    Wagner, R.A.

    1992-06-01

    This report presents a summary of all RE/SPEC Inc. technical support activities to the Repository Technology Program (RTP) from September 1, 1988, through June 30, 1992. The RE/SPEC Inc. activities are grouped into the following categories: project management, project quality assurance (QA), performance assessment (PA), support of the Office of Civilian Radioactive Waste Management (OCRWM) through technical reviews and general assistance, participation in the Department of Energy (DOE) International Program, and code evaluation and documentation.

  6. 32 CFR 701.113 - PA exemptions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... DOCUMENTS AFFECTING THE PUBLIC DON Privacy Program § 701.113 PA exemptions. (a) Exempt systems of records. 5 U.S.C. 552a authorizes SECNAV to adopt rules designating eligible systems of records as exempt from... responsible for proposing an exemption rule. Exempt systems of records are identified at...

  7. 32 CFR 701.113 - PA exemptions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... DOCUMENTS AFFECTING THE PUBLIC DON Privacy Program § 701.113 PA exemptions. (a) Exempt systems of records. 5 U.S.C. 552a authorizes SECNAV to adopt rules designating eligible systems of records as exempt from... responsible for proposing an exemption rule. Exempt systems of records are identified at...

  8. 32 CFR 701.113 - PA exemptions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... DOCUMENTS AFFECTING THE PUBLIC DON Privacy Program § 701.113 PA exemptions. (a) Exempt systems of records. 5 U.S.C. 552a authorizes SECNAV to adopt rules designating eligible systems of records as exempt from... responsible for proposing an exemption rule. Exempt systems of records are identified at...

  9. Program activities, DOE state and local assistance programs, 1980 report

    SciTech Connect

    Chiogioji, Melvin H.

    1981-01-01

    Progress achieved by DOE State and Local Assistance Programs during FY 1980 and since they were established is summarized. These programs enable improved energy efficiency of industry, transportation, commercial establishments, public buildings, and residences. Eight programs (State Energy Conservation, Energy Extension Service, Weatherization Assistance, Institutional Buildings Grants, Energy-Related Inventions, Appropriate Technology Small Grants, Emergency Energy Conservation, Emergency Building Temperature Restrictions) are described. They provide the impetus for thousands of individual and organizational actions that have significantly affected national energy use patterns. (MCW)

  10. National Childhood Diabetes Program Activities in Turkey

    PubMed Central

    Hatun, Şükrü

    2015-01-01

    Recent census figures in Turkey show that out of a population of 76.6 million, 22.7 million (29.7%) are younger than 18 years old. The great majority (>95%) of pediatric cases of diabetes in Turkey are type 1 diabetes mellitus (T1DM). In recent years, with increase in number of pediatric endocrine centers around the country, the important issue of care for diabetic children and adolescents has been revived and major steps have been taken for improvement in pediatric care and its outreach to all diabetic children. The Childhood Diabetes Group continues its activities in cooperation with the Turkish Ministry of Health. A list of areas of interest of the Group include “School programs”, “Incidence/prevalence studies and national registry system”, “Educational guidelines for diabetes in children”, “Increasing the numbers of camps and summer schools for diabetic children”, “Organization of educational programs for the health team”, “National guidelines for transition of diabetic children to adult clinics”, “Improvement of school canteens”, “Educational spots” to improve awareness of diabetes. The activities of the Childhood Diabetes Group will be discussed in detail in this article. PMID:25800469

  11. Exploring Extension Involvement in Farm to School Program Activities

    ERIC Educational Resources Information Center

    Benson, Matthew C.

    2014-01-01

    The study reported here examined Extension professionals' involvement in farm-to-school program activities. Results of an online survey distributed to eight state Extension systems indicate that on average, Extension professionals are involved with one farm to school program activity, with most supporting school or community garden programs.…

  12. An example of activity based costing of treatment programs.

    PubMed

    Alemi, Farrokh; Sullivan, Thomas

    2007-01-01

    We introduce a new tool that can be used for estimating number, length of time, and nature of services patient receive in drug treatment programs. While the field has made significant progress in standardizing the collection of expenditure data, little progress has been made on creating a standard measure for estimating program activities and census. We report on a method of estimating program activities.

  13. Playtime Is Science: Implementing a Parent/Child Activity Program.

    ERIC Educational Resources Information Center

    Sprung, Barbara; And Others

    A program of science activities for children in the early childhood years and their parents is offered. The three different formats of the Playtime Is Science program are adaptable to a variety of settings and schedules. The Parent/Child Activity Program includes one parents-only session in which participants learn that routine chores involve…

  14. A summary of the active flexible wing program

    NASA Technical Reports Server (NTRS)

    Perry, Boyd, III; Cole, Stanley R.; Miller, Gerald D.

    1992-01-01

    A summary of the NASA/Rockwell Active Flexible Wing Program is presented. Major elements of the program are presented. Key program accomplishments included single- and multiple-mode flutter suppression, load alleviation and load control during rapid roll maneuvers, and multi-input/multi-output multiple-function active controls tests above the open-loop flutter boundary.

  15. 40 CFR 5.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 5.400 Education programs or... basis of sex, be excluded from participation in, be denied the benefits of, or be subjected...

  16. 43 CFR 41.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 41.400 Education programs or... basis of sex, be excluded from participation in, be denied the benefits of, or be subjected...

  17. 45 CFR 618.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 618.400 Education programs or... basis of sex, be excluded from participation in, be denied the benefits of, or be subjected...

  18. 10 CFR 5.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 1 2011-01-01 2011-01-01 false Education programs or activities. 5.400 Section 5.400 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs...

  19. 45 CFR 2555.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 4 2011-10-01 2011-10-01 false Education programs or activities. 2555.400 Section... COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  20. 29 CFR 36.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 1 2013-07-01 2013-07-01 false Education programs or activities. 36.400 Section 36.400 Labor Office of the Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs...

  1. 10 CFR 5.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 1 2013-01-01 2013-01-01 false Education programs or activities. 5.400 Section 5.400 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs...

  2. 18 CFR 1317.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 2 2012-04-01 2012-04-01 false Education programs or... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 1317.400 Education...

  3. 38 CFR 23.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2011-07-01 2011-07-01 false Education programs or... (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited §...

  4. 29 CFR 36.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Education programs or activities. 36.400 Section 36.400 Labor Office of the Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs...

  5. 10 CFR 5.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 1 2012-01-01 2012-01-01 false Education programs or activities. 5.400 Section 5.400 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs...

  6. 38 CFR 23.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Education programs or... (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited §...

  7. 24 CFR 3.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Education programs or activities. 3... Urban Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  8. 38 CFR 23.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2014-07-01 2014-07-01 false Education programs or... (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited §...

  9. 38 CFR 23.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2013-07-01 2013-07-01 false Education programs or... (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited §...

  10. 32 CFR 196.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 2 2011-07-01 2011-07-01 false Education programs or activities. 196.400... (CONTINUED) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  11. 24 CFR 3.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 1 2012-04-01 2012-04-01 false Education programs or activities. 3... Urban Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  12. 45 CFR 2555.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 4 2014-10-01 2014-10-01 false Education programs or activities. 2555.400 Section... COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  13. 18 CFR 1317.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 2 2013-04-01 2012-04-01 true Education programs or... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 1317.400 Education...

  14. 45 CFR 2555.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 4 2012-10-01 2012-10-01 false Education programs or activities. 2555.400 Section... COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  15. 7 CFR 15a.31 - Education programs and activities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 1 2012-01-01 2012-01-01 false Education programs and activities. 15a.31 Section 15a.31 Agriculture Office of the Secretary of Agriculture EDUCATION PROGRAMS OR ACTIVITIES RECEIVING OR BENEFITTING FROM FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs...

  16. 7 CFR 15a.31 - Education programs and activities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false Education programs and activities. 15a.31 Section 15a.31 Agriculture Office of the Secretary of Agriculture EDUCATION PROGRAMS OR ACTIVITIES RECEIVING OR BENEFITTING FROM FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs...

  17. 38 CFR 23.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2012-07-01 2012-07-01 false Education programs or... (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited §...

  18. 32 CFR 196.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 2 2013-07-01 2013-07-01 false Education programs or activities. 196.400... (CONTINUED) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  19. 32 CFR 196.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 2 2014-07-01 2014-07-01 false Education programs or activities. 196.400... (CONTINUED) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  20. 45 CFR 2555.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 4 2013-10-01 2013-10-01 false Education programs or activities. 2555.400 Section... COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  1. 32 CFR 196.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Education programs or activities. 196.400... (CONTINUED) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  2. 45 CFR 2555.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Education programs or activities. 2555.400 Section... COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  3. 24 CFR 3.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Education programs or activities. 3... Urban Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  4. 18 CFR 1317.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 2 2010-04-01 2010-04-01 false Education programs or... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 1317.400 Education...

  5. 32 CFR 196.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 2 2012-07-01 2012-07-01 false Education programs or activities. 196.400... (CONTINUED) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  6. 18 CFR 1317.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 2 2014-04-01 2014-04-01 false Education programs or... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 1317.400 Education...

  7. 18 CFR 1317.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 2 2011-04-01 2011-04-01 false Education programs or... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 1317.400 Education...

  8. 24 CFR 3.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false Education programs or activities. 3... Urban Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  9. 10 CFR 5.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 1 2014-01-01 2014-01-01 false Education programs or activities. 5.400 Section 5.400 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs...

  10. 7 CFR 15a.31 - Education programs and activities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 1 2011-01-01 2011-01-01 false Education programs and activities. 15a.31 Section 15a.31 Agriculture Office of the Secretary of Agriculture EDUCATION PROGRAMS OR ACTIVITIES RECEIVING OR BENEFITTING FROM FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs...

  11. 7 CFR 15a.31 - Education programs and activities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 1 2014-01-01 2014-01-01 false Education programs and activities. 15a.31 Section 15a.31 Agriculture Office of the Secretary of Agriculture EDUCATION PROGRAMS OR ACTIVITIES RECEIVING OR BENEFITTING FROM FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs...

  12. 10 CFR 5.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Education programs or activities. 5.400 Section 5.400 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs...

  13. 24 CFR 3.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 1 2013-04-01 2013-04-01 false Education programs or activities. 3... Urban Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  14. Short- and long-term theory-based predictors of physical activity in women who participated in a weight-management program.

    PubMed

    Wasserkampf, A; Silva, M N; Santos, I C; Carraça, E V; Meis, J J M; Kremers, S P J; Teixeira, P J

    2014-12-01

    This study analyzed psychosocial predictors of the Theory of Planned Behavior (TPB) and Self-Determination Theory (SDT) and evaluated their associations with short- and long-term moderate plus vigorous physical activity (MVPA) and lifestyle physical activity (PA) outcomes in women who underwent a weight-management program. 221 participants (age 37.6 ± 7.02 years) completed a 12-month SDT-based lifestyle intervention and were followed-up for 24 months. Multiple linear regression analyses tested associations between psychosocial variables and self-reported short- and long-term PA outcomes. Regression analyses showed that control constructs of both theories were significant determinants of short- and long-term MVPA, whereas affective and self-determination variables were strong predictors of short- and long-term lifestyle PA. Regarding short-term prediction models, TPB constructs were stronger in predicting MVPA, whereas SDT was more effective in predicting lifestyle PA. For long-term models, both forms of PA were better predicted by SDT in comparison to TPB. These results highlight the importance of comparing health behavior theories to identify the mechanisms involved in the behavior change process. Control and competence constructs are crucial during early adoption of structured PA behaviors, whereas affective and intrinsic sources of motivation are more involved in incidental types of PA, particularly in relation to behavioral maintenance.

  15. Making healthy eating and physical activity policy practice: process evaluation of a group randomized controlled intervention in afterschool programs

    PubMed Central

    Weaver, R. Glenn; Beets, Michael W.; Hutto, Brent; Saunders, Ruth P.; Moore, Justin B.; Turner-McGrievy, Gabrielle; Huberty, Jennifer L.; Ward, Dianne S.; Pate, Russell R.; Beighle, Aaron; Freedman, Darcy

    2015-01-01

    This study describes the link between level of implementation and outcomes from an intervention to increase afterschool programs’ (ASPs) achievement of healthy eating and physical activity (HE-PA) Standards. Ten intervention ASPs implemented the Strategies-To-Enhance-Practice (STEPs), a multi-component, adaptive intervention framework identifying factors essential to meeting HE-PA Standards, while 10 control ASPs continued routine practice. All programs, intervention and control, were assigned a STEPs for HE-PA index score based on implementation. Mixed-effects linear regressions showed high implementation ASPs had the greatest percentage of boys and girls achieving 30 min of moderate-to-vigorous physical activity (47.3 and 29.3%), followed by low implementation ASPs (41.3 and 25.0%), and control ASPs (34.8 and 18.5%). For healthy eating, high/low implementation programs served fruits and vegetables an equivalent number of days, but more days than control programs (74.0 and 79.1% of days versus 14.2%). A similar pattern emerged for the percent of days sugar-sweetened foods and beverages were served, with high and low implementation programs serving sugar-sweetened foods (8.0 and 8.4% of days versus 52.2%), and beverages (8.7 and 2.9% of days versus 34.7%) equivalently, but less often than control programs. Differences in characteristics and implementation of STEPs for HE-PA between high/low implementers were also identified. PMID:26590240

  16. How family support affects physical activity (PA) among middle-aged and elderly people before and after they suffer from chronic diseases.

    PubMed

    Yuan, Su-Chuan; Weng, Shuo-Chun; Chou, Ming-Chih; Tang, Yih-Jing; Lee, Shu-Hsin; Chen, Der-Yuan; Chuang, Ya-Wen; Yu, Chia-Hui; Kuo, Hsien-Wen

    2011-01-01

    The more support elderly people have from their family, the less likely they are to suffer from chronic diseases. The objective of this study is to investigate how family support affects the PA middle-aged and elderly people engage in before and after they suffer from chronic diseases. We interviewed 428 middle-aged and elderly people using a structured questionnaire to measure their aerobic PA. Eighteen percent of middle-aged and elderly people did participate in PA after suffering from chronic diseases. Using multivariate logistic regression models, we found that middle-aged and elderly people who rely on family members when they are sick (OR=1.87, 95%CI=1.08-3.25) and who are accompanied by family members (OR=2.09, 95%CI=1.20-3.62) when they are healthy are more likely to exercise. The more middle-aged and elderly people are supported by their family, the more likely they are to exercise. Strengthening family relationships should help reduce the prevalence of chronic diseases among middle-aged and elderly people.

  17. University Program Management Information System: NASA's University Program Active Projects

    NASA Technical Reports Server (NTRS)

    Gans, Gary (Technical Monitor)

    2003-01-01

    As basic policy, NASA believes that colleges and universities should be encouraged to participate in the nation's space and aeronautics program to the maximum extent practicable. Indeed, universities are considered as partners with government and industry in the nation's aerospace program. NASA's objective is to have them bring their scientific, engineering, and social research competence to bear on aerospace problems and on the broader social, economic, and international implications of NASA's technical and scientific programs. It is expected that, in so doing, universities will strengthen both their research and their educational capabilities to contribute more effectively to the national well being. This annual report is one means of documenting the NASA-university relationship, frequently denoted, collectively, as NASA's University Program. This report is consistent with agency accounting records, as the data is obtained from NASA's Financial and Contractual Status (FACS) System, operated by the Financial Management Division and the Procurement Office. However, in accordance with interagency agreements, the orientation differs from that required for financial or procurement purposes. Any apparent discrepancies between this report and other NASA procurement or financial reports stem from the selection criteria for the data. This report was prepared by the Office of Education/N.

  18. The Faith, Activity, and Nutrition Program

    PubMed Central

    Wilcox, Sara; Parrot, Allen; Baruth, Megan; Laken, Marilyn; Condrasky, Margaret; Saunders, Ruth; Dowda, Marsha; Evans, Rebecca; Addy, Cheryl; Warren, Tatiana Y.; Kinnard, Deborah; Zimmerman, Lakisha

    2013-01-01

    Background Faith-based interventions hold promise for promoting health in ethnic minority populations. To date, however, few of these interventions have used a community-based participatory research (CBPR) approach, have targeted both physical activity and healthy eating, and have focused on structural changes in the church. Purpose To report the results of a group randomized CBPR intervention targeting physical activity and healthy eating in African-American churches. Design Group RCT. Data were collected from 2007 to 2011. Statistical analyses were conducted in 2012. Setting/participants Seventy-four African Methodist Episcopal (AME) churches in South Carolina and 1257 members within them participated in the study. Intervention Churches were randomized to an immediate (intervention) or delayed (control) 15-month intervention that targeted organizational and environmental changes consistent with the structural ecologic model. A CBPR approach guided intervention development. Intervention churches attended a full-day committee training and a full-day cook training. They also received a stipend and 15 months of mailings and technical assistance calls to support intervention implementation. Main outcome measures Primary outcomes were self-reported moderate-to-vigorous-intensity physical activity (MVPA), self-reported fruit and vegetable consumption, and measured blood pressure. Secondary outcomes were self-reported fat- and fiber-related behaviors. Measurements were taken at baseline and 15 months. Intent-to-treat repeated measures ANOVA tested group X time interactions, controlling for church clustering, wave, and size, and participant age, gender, and education. Post hoc ANCOVAs were conducted with measurement completers. Results There was a significant effect favoring the intervention group in self-reported leisure-time MVPA (d=0.18, p=0.02), but no effect for other outcomes. ANCOVA analyses showed an intervention effect for self-reported leisure-time MVPA (d=0

  19. Melatonin induces cell apoptosis in Mia PaCa-2 cells via the suppression of nuclear factor-κB and activation of ERK and JNK: A novel therapeutic implication for pancreatic cancer.

    PubMed

    Li, Weimin; Wu, Jiansheng; Li, Zhiyin; Zhou, Zhibo; Zheng, Chao; Lin, Le; Tan, Binbin; Huang, Min; Fan, Mengdi

    2016-11-01

    Melatonin is synthesized by the pineal gland and is released into the blood. In the last several years, some studies have shown that melatonin has anticancer properties; however, the mechanisms behind the antitumour traits are unclear, especially in pancreatic cancer. Therefore, in the present study, we investigated the antitumour effects of melatonin on the human pancreatic carcinoma cell line MIA PaCa‑2 and explored its biological mechanisms. MIA PaCa‑2 cells were treated with melatonin, and we used a CCK‑8 assay to evaluate the cell viability. We also used flow cytometry to observe cell apoptosis and western blot analysis to assess the protein expression. Our study found that melatonin inhibited cell viability, suppressed colony formation and reduced cell migration and invasion and induced cell apoptosis in MIA PaCa‑2 cells. Our results showed that melatonin treatment inhibited NF‑κB p65 activation. Moreover, melatonin treatment activated the mitogen‑activated protein kinase pathways (c‑jun N‑terminal kinase and extracellular‑regulated kinase 1/2), which increased Bax protein expression and caspase‑3 cleavage and decreased Bcl‑2 protein expression. These new developments demonstrate that melatonin plays a potential role in anticancer treatment and may act as an effective therapeutic agent in the future.

  20. Active Duty (AD) Claims Payment Program

    DTIC Science & Technology

    1991-08-26

    Claims Payment Program References: (a) Sections 1073 and 1074(c) of title 10, United States Code (b) DoD 6010.8-R, "Civilian Health and Medical Program...Provider) 1. This payment was calculated under the CHAMPUS DRG-based payment system as directed by Sections 1073 and 1074(c) of title 10, United

  1. 20 CFR 631.51 - Allowable substate program activities.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Allowable substate program activities. 631.51 Section 631.51 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR PROGRAMS UNDER TITLE III OF THE JOB TRAINING PARTNERSHIP ACT Substate Programs § 631.51 Allowable...

  2. 44 CFR 19.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 44 Emergency Management and Assistance 1 2013-10-01 2013-10-01 false Education programs or..., DEPARTMENT OF HOMELAND SECURITY GENERAL NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs...

  3. 44 CFR 19.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Education programs or..., DEPARTMENT OF HOMELAND SECURITY GENERAL NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs...

  4. 36 CFR 1211.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false Education programs or... ADMINISTRATION GENERAL RULES NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  5. 36 CFR 1211.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Education programs or... ADMINISTRATION GENERAL RULES NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  6. 36 CFR 1211.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Education programs or... ADMINISTRATION GENERAL RULES NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  7. 44 CFR 19.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 44 Emergency Management and Assistance 1 2011-10-01 2011-10-01 false Education programs or..., DEPARTMENT OF HOMELAND SECURITY GENERAL NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs...

  8. 36 CFR 1211.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 3 2013-07-01 2012-07-01 true Education programs or... ADMINISTRATION GENERAL RULES NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  9. 44 CFR 19.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 44 Emergency Management and Assistance 1 2014-10-01 2014-10-01 false Education programs or..., DEPARTMENT OF HOMELAND SECURITY GENERAL NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs...

  10. 44 CFR 19.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 44 Emergency Management and Assistance 1 2012-10-01 2011-10-01 true Education programs or..., DEPARTMENT OF HOMELAND SECURITY GENERAL NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs...

  11. 36 CFR 1211.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false Education programs or... ADMINISTRATION GENERAL RULES NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or...

  12. Commercial/Industrial Activities Program: The Effect of Program Implementation at the Command Level.

    DTIC Science & Technology

    1986-12-01

    R,177 468 COMMERCIAL /INDUSTRIAL ACTIVITIES PROGRAM THE EFFECT OF 1/1 PROGRAM IMPLEMENTATION AT THE COMMAND LEVEL(U) NAVAL POSTGRADUATE SCHOOL...OLUTION 1ESI Chs,; o NAVAL POSTGRADUATE SCHOOL Monterey, California rMl , MA. 0 3 1987 ,, THESIS V E COMMERCIAL /INDUSTRIAL ACTIVITIES PROGRAM: THE...block number) ED GROUP SUB-GROUP Commercial Activities; L Effective Implementation 9 ,BS’RAC’ (Continue on reverie if necessary ard ident, fy by block

  13. Program Collaboration and Service Integration Activities Among HIV Programs in 59 U.S. Health Departments

    PubMed Central

    Toledo, Lauren; Dunbar, Erica; Aquino, Gustavo A.; Nesheim, Steven R.

    2014-01-01

    Objectives We identified the level and type of program collaboration and service integration (PCSI) among HIV prevention programs in 59 CDC-funded health department jurisdictions. Methods Annual progress reports (APRs) completed by all 59 health departments funded by CDC for HIV prevention activities were reviewed for collaborative and integrated activities reported by HIV programs for calendar year 2009. We identified associations between PCSI activities and funding, AIDS diagnosis rate, and organizational integration. Results HIV programs collaborated with other health department programs through data-related activities, provider training, and providing funding for sexually transmitted disease (STD) activities in 24 (41%), 31 (53%), and 16 (27%) jurisdictions, respectively. Of the 59 jurisdictions, 57 (97%) reported integrated HIV and STD testing at the same venue, 39 (66%) reported integrated HIV and tuberculosis testing, and 26 (44%) reported integrated HIV and viral hepatitis testing. Forty-five (76%) jurisdictions reported providing integrated education/outreach activities for HIV and at least one other disease. Twenty-six (44%) jurisdictions reported integrated partner services among HIV and STD programs. Overall, the level of PCSI activities was not associated with HIV funding, AIDS diagnoses, or organizational integration. Conclusions HIV programs in health departments collaborate primarily with STD programs. Key PCSI activities include integrated testing, integrated education/outreach, and training. Future assessments are needed to evaluate PCSI activities and to identify the level of collaboration and integration among prevention programs. PMID:24385647

  14. Medically related activities of application team program

    NASA Technical Reports Server (NTRS)

    1971-01-01

    Application team methodology identifies and specifies problems in technology transfer programs to biomedical areas through direct contact with users of aerospace technology. The availability of reengineering sources increases impact of the program on the medical community and results in broad scale application of some bioinstrumentation systems. Examples are given that include devices adapted to the rehabilitation of neuromuscular disorders, power sources for artificial organs, and automated monitoring and detection equipment in clinical medicine.

  15. Process Evaluation of an Intervention to Increase Child Activity Levels in Afterschool Programs

    PubMed Central

    Weaver, R. Glenn; Saunders, Ruth; Webster, Collin; Beets, Michael W.

    2014-01-01

    Background Identifying effective strategies in Afterschool programs (ASPs) to increase children's moderate-to-vigorous physical activity (MVPA) in the ASP setting is crucial. This study describes the process evaluation outcomes from an intervention to reduce child sedentary time and increase MVPA in ASPs. Methods Four ASPs participated in a quasi-experimental single-group pre-post study targeting child sedentary time and MVPA. The strategies implemented to help ASPs meet Physical Activity Standards consisted of detailed schedules, professional development trainings, on-site booster sessions, and technical assistance. Process evaluation related to staff behaviors was collected via systematic observation to identify the interventions impact on the physical and social environment of the ASP. Random-effects regression models examined the impact of the intervention on boys/girls observed sedentary behavior, MVPA, and changes in staff behaviors. Results Increases in MVPA and reductions in sedentary behavior were observed during enrichment, academics, organized and free-play physical activities (PA). Corresponding changes in staff behaviors were observed during these ASP contexts. For example, staff reduced child idle-time during organized PA (38.9%-1.8%) and provided energizers more often during enrichment (0.2%-11.5%). Conclusions This study identified changes in staff behavior during ASP contexts that led to increases in child MVPA and decreases in child sedentary behavior. PMID:24836999

  16. 32 CFR 701.109 - Privacy Act (PA) appeals.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 5 2014-07-01 2014-07-01 false Privacy Act (PA) appeals. 701.109 Section 701.109 National Defense Department of Defense (Continued) DEPARTMENT OF THE NAVY UNITED STATES NAVY... OF THE NAVY DOCUMENTS AFFECTING THE PUBLIC DON Privacy Program § 701.109 Privacy Act (PA) appeals....

  17. 32 CFR 701.109 - Privacy Act (PA) appeals.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 5 2013-07-01 2013-07-01 false Privacy Act (PA) appeals. 701.109 Section 701.109 National Defense Department of Defense (Continued) DEPARTMENT OF THE NAVY UNITED STATES NAVY... OF THE NAVY DOCUMENTS AFFECTING THE PUBLIC DON Privacy Program § 701.109 Privacy Act (PA) appeals....

  18. H-bonded supramolecular assembly via proton transfer: Isolation, X-ray crystallographic characterization and SOD mimic activity of [Cu(dipic)2]2[PA-H]4·5H2O

    NASA Astrophysics Data System (ADS)

    Siddiqi, Zafar A.; Khalid, Mohd; Shahid, M.; Kumar, Sarvendra; Sharma, Prashant K.; Siddique, Armeen; Anjuli

    2013-02-01

    The title proton transfer complex was isolated from the reaction of CuCl2·2H2O with pyridine-2,6-dicarboxylic acid (dipic-H2) in presence of 3-amino-1-propanol (PA) under reflux condition. It was thoroughly characterized by FAB-mass, FT-IR, electronic (ligand field), EPR spectroscopic and X-ray crystallographic techniques. The complex crystallizes in the space group C1c1 of the monoclinic system. The unit cell parameters are, a = 17.247(5), b = 20.058(5) Å, c = 15.320(4) Å and β = 109.235(5)°. Extensive H-bonding between the complex anion [Cu(dipic)2]2- and the surrounding cations [PA-H]+ results in a 3D network, supported with additional π-π interactions of the ligand (dipic)2- neighboring units. The SOD mimic activity of the present complex was also examined using NBT assay.

  19. Perceptions and Evaluation of a Physical Activity Program

    ERIC Educational Resources Information Center

    Smith, Gene A.

    2012-01-01

    This qualitative case study, conducted in the Midwestern United States, explored the perceptions of teachers at two different elementary schools as they implemented a physical activity program during the school day. The program engaged students in daily physical activity through brief, organized, structured physical exercise. Interviews and…

  20. Active Writing Plus Feedback: A Successful Elementary School Writing Program.

    ERIC Educational Resources Information Center

    Vockell, Edward L.; And Others

    The Highland (Indiana) public schools developed a writing program for elementary school students based on the premise that children learn to use language by actively generating language. The program features language production as the principal student activity in the English class and uses the students' own written work and oral expression as the…

  1. 49 CFR 25.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 25.400 Transportation Office of the Secretary of Transportation NONDISCRIMINATION ON THE BASIS OF SEX... Basis of Sex in Education Programs or Activities Prohibited § 25.400 Education programs or activities... basis of sex, be excluded from participation in, be denied the benefits of, or be subjected...

  2. 22 CFR 146.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Section 146.400 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF SEX... Basis of Sex in Education Programs or Activities Prohibited § 146.400 Education programs or activities... basis of sex, be excluded from participation in, be denied the benefits of, or be subjected...

  3. 34 CFR 106.31 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false Education programs or activities. 106.31 Section 106.31 Education Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING...

  4. 34 CFR 106.31 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 1 2011-07-01 2011-07-01 false Education programs or activities. 106.31 Section 106.31 Education Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING...

  5. 34 CFR 106.31 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 1 2012-07-01 2012-07-01 false Education programs or activities. 106.31 Section 106.31 Education Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING...

  6. 34 CFR 106.31 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 1 2014-07-01 2014-07-01 false Education programs or activities. 106.31 Section 106.31 Education Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING...

  7. 34 CFR 106.31 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 1 2013-07-01 2013-07-01 false Education programs or activities. 106.31 Section 106.31 Education Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING...

  8. Pseudomonas aeruginosa phage PaP1 DNA polymerase is an A-family DNA polymerase demonstrating ssDNA and dsDNA 3'-5' exonuclease activity.

    PubMed

    Liu, Binyan; Gu, Shiling; Liang, Nengsong; Xiong, Mei; Xue, Qizhen; Lu, Shuguang; Hu, Fuquan; Zhang, Huidong

    2016-08-01

    Most phages contain DNA polymerases, which are essential for DNA replication and propagation in infected host bacteria. However, our knowledge on phage-encoded DNA polymerases remains limited. This study investigated the function of a novel DNA polymerase of PaP1, which is the lytic phage of Pseudomonas aeruginosa. PaP1 encodes its sole DNA polymerase called Gp90 that was predicted as an A-family DNA polymerase with polymerase and 3'-5' exonuclease activities. The sequence of Gp90 is homologous but not identical to that of other A-family DNA polymerases, such as T7 DNA polymerases (Pol) and DNA Pol I. The purified Gp90 demonstrated a polymerase activity. The processivity of Gp90 in DNA replication and its efficiency in single-dNTP incorporation are similar to those of T7 Pol with processive thioredoxin (T7 Pol/trx). Gp90 can degrade ssDNA and dsDNA in 3'-5' direction at a similar rate, which is considerably lower than that of T7 Pol/trx. The optimized conditions for polymerization were a temperature of 37 °C and a buffer consisting of 40 mM Tris-HCl (pH 8.0), 30 mM MgCl2, and 200 mM NaCl. These studies on DNA polymerase encoded by PaP1 help advance our knowledge on phage-encoded DNA polymerases and elucidate PaP1 propagation in infected P. aeruginosa.

  9. Activities for a Perceptual Motor Program.

    ERIC Educational Resources Information Center

    Brinning, Dorothy; And Others

    Perceptual motor activities for physically handicapped children are presented in the areas of fine and gross motor skills. Also detailed are activities to develop body image, visual motor skills, and tactile and auditory perception. (JD)

  10. Conservation Seeds Activities Book. An Early Childhood Conservation Education Program.

    ERIC Educational Resources Information Center

    Griffin, Sherri

    This activities book is used with an early childhood conservation education program. The activities are presented in four color-coded sections, each section representing one of the four seasons. Each activity includes a statement of purpose, list of materials needed, instructional strategies, and a list of supplementary activities. In addition to…

  11. A Test of Social Cognitive Theory to Explain Men's Physical Activity During a Gender-Tailored Weight Loss Program.

    PubMed

    Young, Myles D; Plotnikoff, Ronald C; Collins, Clare E; Callister, Robin; Morgan, Philip J

    2016-11-01

    Physical inactivity is a leading contributor to the burden of disease in men. Social-cognitive theories may improve physical activity (PA) interventions by identifying which variables to target to maximize intervention impact. This study tested the utility of Bandura's social cognitive theory (SCT) to explain men's PA during a 3-month weight loss program. Participants were 204 overweight/obese men (M [SD] age = 46.6 [11.3] years; body mass index = 33.1 [3.5] kg/m(2)). A longitudinal, latent variable structural equation model tested the associations between SCT constructs (i.e., self-efficacy, outcome expectations, intention, and social support) and self-reported moderate-to-vigorous PA (MVPA) and examined the total PA variance explained by SCT. After controlling for Time 1 cognitions and behavior, the model fit the data well (χ(2) = 73.9, degrees of freedom = 39, p < .001; normed χ(2) = 1.9; comparative fit index = 0.96; standardized root mean residual = 0.059) and explained 65% of the variance in MVPA at Time 2. At Time 2, self-efficacy demonstrated the largest direct and total effects on MVPA (βdirect = .45, p < .001; βtotal = .67, p = .002). A small-to-medium effect was observed from intention to MVPA, but not from outcome expectations or social support. This study provides some evidence supporting the tenets of SCT when examining PA behavior in overweight and obese men. Future PA and weight loss interventions for men may benefit by targeting self-efficacy and intention, but the utility of targeting social support and outcome expectations requires further examination.

  12. Overview of the Novel Intelligent JAXA Active Rotor Program

    NASA Technical Reports Server (NTRS)

    Saito, Shigeru; Kobiki, Noboru; Tanabe, Yasutada; Johnson, Wayne; Yamauchi, Gloria K.; Young, Larry A.

    2010-01-01

    The Novel Intelligent JAXA Active Rotor (NINJA Rotor) program is a cooperative effort between JAXA and NASA, involving a test of a JAXA pressure-instrumented, active-flap rotor in the 40- by 80-Foot Wind Tunnel at Ames Research Center. The objectives of the program are to obtain an experimental database of a rotor with active flaps and blade pressure instrumentation, and to use that data to develop analyses to predict the aerodynamic and aeroacoustic performance of rotors with active flaps. An overview of the program is presented, including a description of the rotor and preliminary pretest calculations.

  13. USGS Mineral Resources Program: International Activities

    USGS Publications Warehouse

    Kropschot, S.J.

    1998-01-01

    The USGS is the country's leading earth science organization. Since 1879, USGS scientists have gathered and analyzed data and disseminated the results of their research on the geology, cartography, hydrology, and, more recently biology, of every continent and ocean on Earth. Multidisciplinary research both in the United States and in the international arena has been an important part of the USGS mission. The USGS Mineral Resources Program is the sole Federal agency program that provides high-quality scientific information, objective resource assessments, and unbiased research results on mineral issues

  14. 32 CFR 701.124 - PA self assessments/inspections.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... SECNAVINST 5210.8D). These reports shall be made available to PA program officials and to CNO (DNS-36)/CMC... until the next self-assessment is completed. Make these reports available, upon request, to CNO...

  15. MLS student active learning within a "cloud" technology program.

    PubMed

    Tille, Patricia M; Hall, Heather

    2011-01-01

    In November 2009, the MLS program in a large public university serving a geographically large, sparsely populated state instituted an initiative for the integration of technology enhanced teaching and learning within the curriculum. This paper is intended to provide an introduction to the system requirements and sample instructional exercises used to create an active learning technology-based classroom. Discussion includes the following: 1.) define active learning and the essential components, 2.) summarize teaching methods, technology and exercises utilized within a "cloud" technology program, 3.) describe a "cloud" enhanced classroom and programming 4.) identify active learning tools and exercises that can be implemented into laboratory science programs, and 5.) describe the evaluation and assessment of curriculum changes and student outcomes. The integration of technology in the MLS program is a continual process and is intended to provide student-driven active learning experiences.

  16. The relationship of physical activity (PA) and walking with sarcopenia in Korean males aged 60 years and older using the Fourth Korean National Health and Nutrition Examination Survey (KNHANES IV-2, 3), 2008-2009.

    PubMed

    Kim, Sang-Hwan; Kim, Tae-Ho; Hwang, Hee-Jin

    2013-01-01

    Although a variety of studies have reported the association between age related changes in muscle mass and the functional impairments of the elderly, few have reported on the modifiable lifestyle factors that are related to sarcopenia. Three thousand five hundred ninety-eight men in nationally representative cross-sectional, population-representative sampled survey using data from the KNHANES IV aged 19 years and older who completed a body composition using a dual energy X-ray absorptiometry (DXA) were measured. To determine whether there are any associations between physical activities, nutritional status and sarcopenia were examined in 1156 men among those over 60 years of age. Appendicular skeletal muscle mass (ASM) was calculated as the sum of skeletal muscle in the arms and legs. Sarcopenia was defined as the ASM/Ht(2) of less than two standard deviations (SD) below the sex-specific normal mean for the younger reference group. The prevalence of sarcopenia in Korean men aged 60 years and older was 9.9%. Sarcopenia was negatively associated with the third quartile (OR=0.40, 95% CI=0.20-0.80) and fourth quartile of PA (OR=0.24, 95% CI=0.11-0.52), and negatively associated with the highest quartile of protein intake (OR=0.32, 95% CI=0.14-0.76). In logistic regression models, sarcopenia was negatively associated with walking PA (OR=0.49, 95% CI=0.29-0.83) after an adjustment for age, smoking habits, alcohol drinking, family income, education status and protein intake. Sarcopenia was associated with PA, especially walking PA.

  17. Promoting Physical Activity in Afterschool Programs

    ERIC Educational Resources Information Center

    Beighle, Aaron; Beets, Michael W.; Erwin, Heather E.; Huberty, Jennifer; Moore, Justin B.; Stellino, Megan

    2010-01-01

    Children in the United States are not engaging in sufficient amounts of routine physical activity, and this lack is an emerging public health concern (Strong, Malina, Blimkie, Daniels, Dishman, Gutin, et al., 2005). Efforts to increase the physical activity levels of children and adolescents has become a national priority, attracting attention…

  18. Environmental Education Activities & Programs 1998-1999.

    ERIC Educational Resources Information Center

    Bureau of Reclamation (Dept. of Interior), Denver, CO.

    This document features descriptions of interactive learning models and presentations in environmental education concerning groundwater, geology, the environment, weather, water activities, and interactive games. Activities include: (1) GW-Standard; (2) GW-w/no Leaky Underground Storage Tank (No UST); (3) GW-Karst; (4) GW-Landfill Models--Standard…

  19. Active microwave users working group program planning

    NASA Technical Reports Server (NTRS)

    Ulaby, F. T.; Bare, J.; Brown, W. E., Jr.; Childs, L. F.; Dellwig, L. F.; Heighway, J. E.; Joosten, R.; Lewis, A. J.; Linlor, W.; Lundien, J. R.

    1978-01-01

    A detailed programmatic and technical development plan for active microwave technology was examined in each of four user activities: (1) vegetation; (2) water resources and geologic applications, and (4) oceanographic applications. Major application areas were identified, and the impact of each application area in terms of social and economic gains were evaluated. The present state of knowledge of the applicability of active microwave remote sensing to each application area was summarized and its role relative to other remote sensing devices was examined. The analysis and data acquisition techniques needed to resolve the effects of interference factors were reviewed to establish an operational capability in each application area. Flow charts of accomplished and required activities in each application area that lead to operational capability were structured.

  20. Department of Defense Offshore Military Activities Program

    DTIC Science & Technology

    1987-03-16

    joint use of offshore areas for military and mineral exploration or developmental purposes. (See enclosure 2.) In carrying out negotiations with elements...that from time to time and from place to place the requirements for mineral exploration /development and defense related activities may conflict. In...area, certain defense- related activities on the OCS may be irreconcilable with mineral exploration / development and will, under the procedures

  1. Effect of a school-based intervention on physical activity and quality of life through serial mediation of social support and exercise motivation: the PESSOA program.

    PubMed

    Quaresma, A M; Palmeira, A L; Martins, S S; Minderico, C S; Sardinha, L B

    2014-12-01

    The aim of this study was to explore the effects of social support and behavioral regulation of exercise on physical activity (PA) and quality of life (QoL), in a Portuguese school-based intervention. We hypothesized that serial mediation effects would be present leading to greater levels of PA and QoL. The sample comprised 1042 students (549 boys), aged 10-16 years, BMI = 19.31 ± 3.51, allocated to two groups of schools: control (n = 207) and intervention (n = 835). This study will report the 24 months results of the program, which aimed to develop healthy lifestyles. Questionnaires were used to measure PA, QoL, motivation to exercise and social support. There was no direct impact of the intervention on QoL or PA. Serial mediation analyses were conducted. Social support (P < 0.019) and intrinsic motivation (P = 0.085) increased more on intervention group. Indirect effects were observed in all serial mediation models. The positive indirect effects on PA and QoL were explained by the increase on peer/parent support in serial with the increase in intrinsic motivation (P < 0.01). Parental support led to an increase on external motivation (P < 0.05), which buffered the effects of the intervention. This school-based intervention promoted the development of social support and motivational mechanisms that explained higher levels of PA and QoL.

  2. Positive Impact on Physical Activity and Health Behaviour Changes of a 15-Week Family Focused Intervention Program: “Juniors for Seniors”

    PubMed Central

    Bronikowska, Małgorzata; Pluta, Beata; Maciaszek, Janusz; Tomczak, Maciej

    2016-01-01

    The promotion of physical activity (PA) in children and their parents requires effective planning and sometimes even interventions. This study shows the effect of PA during a 15-week intervention program “Junior for Seniors” by applying a socioecological model to the interpretation of the data. This comprehensive approach emphasizes the fact that health promotion should focus not only on intrapersonal factors but also on the multilevel factors that might be determinants and modulators of increased PA. In 2015, 24 children (“juniors,” 14 girls and 10 boys, aged M = 7.96 ± 0.69) and 22 parents (“seniors,” 14 mothers aged M = 38.86 ± 2.96 and 8 fathers aged M = 37.38 ± 2.97) were voluntarily enrolled in a study spread across three primary schools in the city of Poznań, Poland. The effectiveness of the intervention was determined according to postintervention behavioural changes in PA in comparison to preintervention levels, as reported by the parents and children. Overall, the study found increases in PA levels and reductions in sedentary time. Although the changes are modest, there are some unrecognized benefits of the intervention which may have occurred, such as improved sport and motor skills, more frequent family social behaviours (walks, meals, and visiting relatives), or simply improved quality of “do-together” leisure time PA. PMID:27766262

  3. Effects of an Individualized Activity Program on Elderly Patients

    ERIC Educational Resources Information Center

    Salter, Carlota de Lerma; Salter, Charles A.

    1975-01-01

    A combined program of Reality Orientation, Activities of Daily Living, and Recreational Activities, together with environmental stimulation, was applied on an individualized basis to 21 elderly patients suffering from both psychological disorders and long-term physical illness. The motivation to participate in the available activities increased…

  4. Mission Impossible? Physical Activity Programming for Individuals Experiencing Homelessness

    ERIC Educational Resources Information Center

    Gregg, Melanie J.; Bedard, Andrea

    2016-01-01

    Purpose: A pilot study was conducted to describe the physical activity experiences and perceived benefits of and barriers to physical activity participation for patrons of a homeless shelter. The resulting pilot data may be used to inform the creation of and support for physical activity and sport programs for those experiencing homelessness.…

  5. Participation in Extracurricular Physical Activity Programs at Middle Schools.

    ERIC Educational Resources Information Center

    Powers, Holly S.; Conway, Terry L.; McKenzie, Thomas L.; Sallis, James F.; Marshall, Simon J.

    2002-01-01

    Evaluated student participation in structured, on-campus extracurricular activities at 24 middle schools. Overall, boys and girls participated at similar rates (except for intramurals). All schools offered multiple extracurricular activity programs, but due to low participation rates, the amount of physical activity obtained was minimal.…

  6. Improving Nutrition and Physical Activity Policies in Afterschool Programs: Results from a Group-Randomized Controlled Trial

    PubMed Central

    Kenney, Erica L.; Giles, Catherine M.; deBlois, Madeleine E.; Gortmaker, Steven L.; Chinfatt, Sherene; Cradock, Angie L.

    2017-01-01

    OBJECTIVE Afterschool programs can be health-promoting environments for children. Written policies positively influence nutrition and physical activity (PA) environments, but effective strategies for building staff capacity to write such policies have not been evaluated. This study measures the comprehensiveness of written nutrition, PA, and screen time policies in afterschool programs and assesses impact of the Out of School Nutrition and Physical Activity (OSNAP) intervention on key policies. METHODS Twenty afterschool programs in Boston, MA participated in a group-randomized, controlled trial from September 2010 to June 2011. Intervention program staff attended learning collaboratives focused on practice and policy change. The Out-of-School Time (OST) Policy Assessment Index evaluated written policies. Inter-rater reliability and construct validity of the measure and impact of the intervention on written policies were assessed. RESULTS The measure demonstrated moderate to excellent inter-rater reliability (Spearman’s r=0.53 to 0.97) and construct validity. OSNAP was associated with significant increases in standards-based policy statements surrounding snacks (+2.6, p=0.003), beverages (+2.3, p=0.008), screen time (+0.8, p=0.046), family communication (+2.2, p=0.002), and a summary index of OSNAP goals (+3.3, p=0.02). CONCLUSIONS OSNAP demonstrated success in building staff capacity to write health-promoting policy statements. Future research should focus on determining policy change impact on practices. PMID:24941286

  7. Update on Program Review Activities. Report 10-11

    ERIC Educational Resources Information Center

    Jones, Jessika

    2010-01-01

    Review of new degree and certificate programs proposed by the public higher education systems is a major California Postsecondary Education Commission (CPEC) activity. The Legislature, Administration, and the Legislative Analyst's Office consider program review to be one of CPEC's most important responsibilities. In the past six months, staff have…

  8. Implications for Comprehensive School Physical Activity Program Implementation

    ERIC Educational Resources Information Center

    Karp, Grace Goc; Scruggs, Philip W.; Brown, Helen; Kelder, Steven H.

    2014-01-01

    As mentioned in the introduction, Physical Education Teacher Education (PETE) programs and physical education teachers will need to identify and resolve the tensions of shifting from a traditional role of a self-contained physical education program to that of an expanding role of physical education that supports lifelong physical activity in…

  9. Assessing Sustainability of Lifestyle Education for Activity Program (LEAP)

    ERIC Educational Resources Information Center

    Saunders, R. P.; Pate, R. R.; Dowda, M.; Ward, D. S.; Epping, J. N.; Dishman, R. K.

    2012-01-01

    Sustained intervention effects are needed for positive health impacts in populations; however, few published examples illustrate methods for assessing sustainability in health promotion programs. This paper describes the methods for assessing sustainability of the Lifestyle Education for Activity Program (LEAP). LEAP was a comprehensive…

  10. 29 CFR 36.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Labor Office of the Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or... these Title IX regulations, no person shall, on the basis of sex, be excluded from participation in,...

  11. 29 CFR 36.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Labor Office of the Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or... these Title IX regulations, no person shall, on the basis of sex, be excluded from participation in,...

  12. 29 CFR 36.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Labor Office of the Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or... these Title IX regulations, no person shall, on the basis of sex, be excluded from participation in,...

  13. 20 CFR 631.51 - Allowable substate program activities.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 3 2011-04-01 2011-04-01 false Allowable substate program activities. 631.51 Section 631.51 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR PROGRAMS... and English for non-English speakers training, retraining services provided with funds available to...

  14. Antagonistic Activity and Mode of Action of Phenazine-1-Carboxylic Acid, Produced by Marine Bacterium Pseudomonas aeruginosa PA31x, Against Vibrio anguillarum In vitro and in a Zebrafish In vivo Model.

    PubMed

    Zhang, Linlin; Tian, Xueying; Kuang, Shan; Liu, Ge; Zhang, Chengsheng; Sun, Chaomin

    2017-01-01

    Phenazine and its derivatives are very important secondary metabolites produced from Pseudomonas spp. and have exhibited broad-spectrum antifungal and antibacterial activities. However, till date, there are few reports about marine derived Pseudomonas and its production of phenazine metabolites. In this study, we isolated a marine Pseudomonas aeruginosa strain PA31x which produced natural product inhibiting the growth of Vibrio anguillarum C312, one of the most serious bacterial pathogens in marine aquaculture. Combining high-resolution electro-spray-ionization mass spectroscopy and nuclear magnetic resonance spectroscopy analyses, the functional compound against V. anguillarum was demonstrated to be phenazine-1-carboxylic acid (PCA), an important phenazine derivative. Molecular studies indicated that the production of PCA by P. aeruginosa PA31x was determined by gene clusters phz1 and phz2 in its genome. Electron microscopic results showed that treatment of V. anguillarum with PCA developed complete lysis of bacterial cells with fragmented cytoplasm being released to the surrounding environment. Additional evidence indicated that reactive oxygen species generation preceded PCA-induced microbe and cancer cell death. Notably, treatment with PCA gave highly significant protective activities against the development of V. anguillarum C312 on zebrafish. Additionally, the marine derived PCA was further found to effectively inhibit the growth of agricultural pathogens, Acidovorax citrulli NP1 and Phytophthora nicotianae JM1. Taken together, this study reveals that marine Pseudomonas derived PCA carries antagonistic activities against both aquacultural and agricultural pathogens, which broadens the application fields of PCA.

  15. Antagonistic Activity and Mode of Action of Phenazine-1-Carboxylic Acid, Produced by Marine Bacterium Pseudomonas aeruginosa PA31x, Against Vibrio anguillarum In vitro and in a Zebrafish In vivo Model

    PubMed Central

    Zhang, Linlin; Tian, Xueying; Kuang, Shan; Liu, Ge; Zhang, Chengsheng; Sun, Chaomin

    2017-01-01

    Phenazine and its derivatives are very important secondary metabolites produced from Pseudomonas spp. and have exhibited broad-spectrum antifungal and antibacterial activities. However, till date, there are few reports about marine derived Pseudomonas and its production of phenazine metabolites. In this study, we isolated a marine Pseudomonas aeruginosa strain PA31x which produced natural product inhibiting the growth of Vibrio anguillarum C312, one of the most serious bacterial pathogens in marine aquaculture. Combining high-resolution electro-spray-ionization mass spectroscopy and nuclear magnetic resonance spectroscopy analyses, the functional compound against V. anguillarum was demonstrated to be phenazine-1-carboxylic acid (PCA), an important phenazine derivative. Molecular studies indicated that the production of PCA by P. aeruginosa PA31x was determined by gene clusters phz1 and phz2 in its genome. Electron microscopic results showed that treatment of V. anguillarum with PCA developed complete lysis of bacterial cells with fragmented cytoplasm being released to the surrounding environment. Additional evidence indicated that reactive oxygen species generation preceded PCA-induced microbe and cancer cell death. Notably, treatment with PCA gave highly significant protective activities against the development of V. anguillarum C312 on zebrafish. Additionally, the marine derived PCA was further found to effectively inhibit the growth of agricultural pathogens, Acidovorax citrulli NP1 and Phytophthora nicotianae JM1. Taken together, this study reveals that marine Pseudomonas derived PCA carries antagonistic activities against both aquacultural and agricultural pathogens, which broadens the application fields of PCA. PMID:28289406

  16. Modulation of macrophage activation and programming in immunity.

    PubMed

    Liu, Guangwei; Yang, Hui

    2013-03-01

    Macrophages are central mediators of the immune, contributing both to the initiation and the resolution of inflammation. The concept of macrophage activation and program has stimulated interest in its definition, and functional significance in homeostasis and diseases. It has been known that macrophages could be differently activated and programmed into different functional subtypes in response to different types of antigen stumuli or different kinds of cytokines present in the microenvironment and could thus profoundly influence immune responses, but little is known about the state and exact regulatory mechanism of macrophage activation and program from cell or molecular signaling level in immunity. In this review, we summarize the recent finding regarding the regulatory mechanism of macrophage activation and program toward M1 and M2, especially on M2 macrophages.

  17. The Pilot Contracting Activities Program: A Management Review

    DTIC Science & Technology

    1988-12-01

    NAVAL POSTGRADUATE SCHOOL Monterey, California SI STA? RADO , THESIS THE PILOT CONTRACTING ACTIVITIES PROGRAM: A MANAGEMENT REVIEW by Robert John ...Approved for public release; distribution is unlimited The Pilot Contracting Activities Program : A Management Review by Robert John Palmquist...the degree of MASTER OF SCIENCE IN MANAGEMENT from the NAVAL POSTGRADUATE SCHOOL December 1988 Author: ’ Y / " Rober John Palmquist Approved by: g17 A c

  18. Activity Based Astronomy for Primary Science Programs.

    ERIC Educational Resources Information Center

    Ginns, Ian

    Print materials in astronomy such as books, journals, charts, and posters are typically the sources of information for teachers and children about the moon, the sun, lunar and solar eclipses, planetary sizes, distances of planets from the sun, planetary atmospheres, and so on. This paper describes and analyzes a number of activities designed to…

  19. Summary of NASA Aerospace Flight Battery Systems Program activities

    NASA Technical Reports Server (NTRS)

    Manzo, Michelle; Odonnell, Patricia

    1994-01-01

    A summary of NASA Aerospace Flight Battery Systems Program Activities is presented. The NASA Aerospace Flight Battery Systems Program represents a unified NASA wide effort with the overall objective of providing NASA with the policy and posture which will increase the safety, performance, and reliability of space power systems. The specific objectives of the program are to: enhance cell/battery safety and reliability; maintain current battery technology; increase fundamental understanding of primary and secondary cells; provide a means to bring forth advanced technology for flight use; assist flight programs in minimizing battery technology related flight risks; and ensure that safe, reliable batteries are available for NASA's future missions.

  20. Repository Technology Program activities, FY 1988

    SciTech Connect

    Yow, J.L. Jr.; Wijesinghe, A.M.; Thorpe, R.K.; Knapp, R.B.

    1989-07-01

    Our technical activities in FY 1988 included instrument selection and evaluation, calculational work, and simulator development. Near the end of the fiscal year, we began preparing several topical reports to document our results. This fiscal year, we continued developing three-dimensional numerical simulators to model coupled hydrologic-and mechanical-rock mass responses and, thus, to provide representative numerical tools for understanding and calculating these in situ processes. We also began scoping calculations in the second half of FY 1988 to evaluate ERE design criteria, but this work was redirected late in the year when the DOE/AECL Subsidiary Agreement was set aside. Our work in developing and evaluating experimental techniques focused on total pressure measurements, moisture content measurement, and tracer detection instrumentation for sealing experiments and for rock-mass-response field tests. At the end of the fiscal year, we completed a review of measurement technology for instrumenting migration/sorption tests to help define the technological requirements in these areas. By the end of FY 1988, we had completed a review of the existing codes for simulating reactive transport; we are using the results of this review to help formulate plans for future activities in this area. The following sections describe the major RTP tasks and activities at LLNL in more detail, and they include our FY 1988 accomplishments in these areas. 8 refs., 22 figs.

  1. Program Activity/Training Plans. STIP II (Skill Training Improvement Programs Round II).

    ERIC Educational Resources Information Center

    Los Angeles Community Coll. District, CA.

    Detailed operational guidelines, training objectives, and learning activities are provided for the Los Angeles Community College District's Skill Training Improvement Programs (STIP II), which are designed to train students for immediate employment. The first of four reports covers Los Angeles Southwest College's computer programming trainee…

  2. Nondiscrimination in Health Programs and Activities. Final rule.

    PubMed

    2016-05-18

    This final rule implements Section 1557 of the Affordable Care Act (ACA) (Section 1557). Section 1557 prohibits discrimination on the basis of race, color, national origin, sex, age, or disability in certain health programs and activities. The final rule clarifies and codifies existing nondiscrimination requirements and sets forth new standards to implement Section 1557, particularly with respect to the prohibition of discrimination on the basis of sex in health programs other than those provided by educational institutions and the prohibition of various forms of discrimination in health programs administered by the Department of Health and Human Services (HHS or the Department) and entities established under Title I of the ACA. In addition, the Secretary is authorized to prescribe the Department's governance, conduct, and performance of its business, including, here, how HHS will apply the standards of Section 1557 to HHS-administered health programs and activities.

  3. Report of the Integrated Program Planning Activity for the DOE Fusion Energy Sciences Program

    SciTech Connect

    2000-12-01

    This report of the Integrated Program Planning Activity (IPPA) has been prepared in response to a recommendation by the Secretary of Energy Advisory Board that, ''Given the complex nature of the fusion effort, an integrated program planning process is an absolute necessity.'' We, therefore, undertook this activity in order to integrate the various elements of the program, to improve communication and performance accountability across the program, and to show the inter-connectedness and inter-dependency of the diverse parts of the national fusion energy sciences program. This report is based on the September 1999 Fusion Energy Sciences Advisory Committee's (FESAC) report ''Priorities and Balance within the Fusion Energy Sciences Program''. In its December 5,2000, letter to the Director of the Office of Science, the FESAC has reaffirmed the validity of the September 1999 report and stated that the IPPA presents a framework and process to guide the achievement of the 5-year goals listed in the 1999 report. The National Research Council's (NRC) Fusion Assessment Committee draft final report ''An Assessment of the Department of Energy's Office of Fusion Energy Sciences Program'', reviewing the quality of the science in the program, was made available after the IPPA report had been completed. The IPPA report is, nevertheless, consistent with the recommendations in the NRC report. In addition to program goals and the related 5-year, 10-year, and 15-year objectives, this report elaborates on the scientific issues associated with each of these objectives. The report also makes clear the relationships among the various program elements, and cites these relationships as the reason why integrated program planning is essential. In particular, while focusing on the science conducted by the program, the report addresses the important balances between the science and energy goals of the program, between the MFE and IFE approaches, and between the domestic and international aspects

  4. Classroom-Directed Home Training Activities. Preschool Program: A Regional Demonstration Program for Preschool Handicapped Children.

    ERIC Educational Resources Information Center

    Jones, Jacquelyn O.

    One of 10 documents developed for preschool programs for handicapped children, the manual presents classroom directed home training activities. The activities are based on such principles as the effectiveness of home instruction by a parent and the need for a parent to feel responsibility for the child's learning. Intended to provide teachers of…

  5. Fidelity to Theory in PA Intervention Research.

    PubMed

    Keller, Colleen; Fleury, Julie; Sidani, Souraya; Ainsworth, Barbara

    2009-04-01

    Research using theory-based interventions to promote regular physical activity (PA) has increased substantially over the past decade. The purpose of this article is to provide a review and summary of PA intervention research specific to fidelity to intervention theory, providing an overview of the concept of fidelity to intervention theory, defining the evaluative components of fidelity: (a) conceptualization of the problem, (b) operationalization of the theory, (c) specification of mediating processes, and (d) specification of outcome variables. Using journal scans and computerized literature database searches, the authors identified 470 PA activity intervention studies that incorporated a theoretical perspective. A validity framework explicated by was used to summarize intervention research in light of fidelity to intervention theory. In all, 15 intervention studies met the inclusion criteria and were incorporated into the review. Theoretical models for health behavior change, including social cognitive theory (SCT), cognitive behavioral theory (CBT), the transtheoretical model (TTM, the TTM combined with SCT), the reversal theory (theory of psychological reversals), and the disconnected values theory, were used as a basis for intervention design and evaluation.

  6. 23 CFR Appendix F to Part 1200 - PLANNING AND ADMINISTRATION (P&A) COSTS

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... (P&A) costs are those direct and indirect costs that are attributable to the management of the... rental costs specific to the Highway Safety Agency. Program management costs are those costs attributable... salary and related costs may be programmed to P&A. If the employee works performing program...

  7. 23 CFR Appendix F to Part 1200 - Planning and Administration (P&A) Costs

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... (P&A) costs are those direct and indirect costs that are attributable to the management of the... rental costs specific to the Highway Safety Agency. Program management costs are those costs attributable... salary and related costs may be programmed to P&A. If the employee works performing program...

  8. Sliding friction study of the oscillating translational motion for steel on PA66 and PA46 type materials

    NASA Astrophysics Data System (ADS)

    Lates, M. T.; Velicu, R. G.; Papuc, R.

    2016-08-01

    The paper presents the study of the friction, by using tests, in the sliding translational oscillating motion of the steel on PA46 relative to the steel on PA66 type materials by considering as variables the testing parameters as: the load, the velocity and the operating temperature. The paper starts with a study of the literature and, according to that, presents the main conclusions regarding the sliding friction of the steel on PA66 and PA46 type materials and identifies the lacks of the results. The tests are performed on an oscillating motion type tribometer module. First, it is made a running-in program, for each of the materials, at 25 and 90°C, a load of 5 N and a frequency of 1 Hz for one hour; after that, there are performed tests at 90°C and 120°C, at loads of 3 N, 5 N, 7 N and at frequencies at 0.25 Hz, 5 Hz and 1 Hz. The results are presented for the PA46 type material relative to the PA66 material. The conclusions indicate in which conditions and with which advantages are used the PA66 and PA46 polyamides in the case of oscillating translational motions.

  9. Developing an active implementation model for a chronic disease management program

    PubMed Central

    Smidth, Margrethe; Christensen, Morten Bondo; Olesen, Frede; Vedsted, Peter

    2013-01-01

    Background Introduction and diffusion of new disease management programs in healthcare is usually slow, but active theory-driven implementation seems to outperform other implementation strategies. However, we have only scarce evidence on the feasibility and real effect of such strategies in complex primary care settings where municipalities, general practitioners and hospitals should work together. The Central Denmark Region recently implemented a disease management program for chronic obstructive pulmonary disease (COPD) which presented an opportunity to test an active implementation model against the usual implementation model. The aim of the present paper is to describe the development of an active implementation model using the Medical Research Council’s model for complex interventions and the Chronic Care Model. Methods We used the Medical Research Council’s five-stage model for developing complex interventions to design an implementation model for a disease management program for COPD. First, literature on implementing change in general practice was scrutinised and empirical knowledge was assessed for suitability. In phase I, the intervention was developed; and in phases II and III, it was tested in a block- and cluster-randomised study. In phase IV, we evaluated the feasibility for others to use our active implementation model. Results The Chronic Care Model was identified as a model for designing efficient implementation elements. These elements were combined into a multifaceted intervention, and a timeline for the trial in a randomised study was decided upon in accordance with the five stages in the Medical Research Council’s model; this was captured in a PaTPlot, which allowed us to focus on the structure and the timing of the intervention. The implementation strategies identified as efficient were use of the Breakthrough Series, academic detailing, provision of patient material and meetings between providers. The active implementation model was

  10. The aircraft energy efficiency active controls technology program

    NASA Technical Reports Server (NTRS)

    Hood, R. V., Jr.

    1977-01-01

    Broad outlines of the NASA Aircraft Energy Efficiency Program for expediting the application of active controls technology to civil transport aircraft are presented. Advances in propulsion and airframe technology to cut down on fuel consumption and fuel costs, a program for an energy-efficient transport, and integrated analysis and design technology in aerodynamics, structures, and active controls are envisaged. Fault-tolerant computer systems and fault-tolerant flight control system architectures are under study. Contracts with leading manufacturers for research and development work on wing-tip extensions and winglets for the B-747, a wing load alleviation system, elastic mode suppression, maneuver-load control, and gust alleviation are mentioned.

  11. Apollo Program Summary Report: Synopsis of the Apollo Program Activities and Technology for Lunar Exploration

    NASA Technical Reports Server (NTRS)

    1975-01-01

    Overall program activities and the technology developed to accomplish lunar exploration are discussed. A summary of the flights conducted over an 11-year period is presented along with specific aspects of the overall program, including lunar science, vehicle development and performance, lunar module development program, spacecraft development testing, flight crew summary, mission operations, biomedical data, spacecraft manufacturing and testing, launch site facilities, equipment, and prelaunch operations, and the lunar receiving laboratory. Appendixes provide data on each of the Apollo missions, mission type designations, spacecraft weights, records achieved by Apollo crewmen, vehicle histories, and a listing of anomalous hardware conditions noted during each flight beginning with Apollo 4.

  12. Anti-listeria activity of poly(lactic acid)/sawdust particle biocomposite film impregnated with pediocin PA-1/AcH and its use in raw sliced pork.

    PubMed

    Woraprayote, Weerapong; Kingcha, Yutthana; Amonphanpokin, Pannawit; Kruenate, Jittiporn; Zendo, Takeshi; Sonomoto, Kenji; Benjakul, Soottawat; Visessanguan, Wonnop

    2013-10-15

    A novel poly(lactic acid) (PLA)/sawdust particle (SP) biocomposite film with anti-listeria activity was developed by incorporation of pediocin PA-1/AcH (Ped) using diffusion coating method. Sawdust particle played an important role in embedding pediocin into the hydrophobic PLA film. The anti-listeria activity of the PLA/SP biocomposite film incorporated with Ped (PLA/SP+Ped) was detected, while no activity against the tested pathogen was observed for the control PLA films (without SP and/or Ped). Dry-heat treatment of film before coating with Ped resulted in the highest Ped adsorption (11.63 ± 3.07 μg protein/cm(2)) and the highest anti-listeria activity. A model study of PLA/SP+Ped as a food-contact antimicrobial packaging on raw sliced pork suggests a potential inhibition of Listeria monocytogenes (99% of total listerial population) on raw sliced pork during the chilled storage. This study supports the feasibility of using PLA/SP+Ped film to reduce the initial load of L. monocytogenes on the surface of raw pork.

  13. 25 CFR 170.115 - What activities may be funded with IRR Program funds?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false What activities may be funded with IRR Program funds? 170... RESERVATION ROADS PROGRAM Indian Reservation Roads Program Policy and Eligibility Eligible Uses If Irr Program Funds § 170.115 What activities may be funded with IRR Program funds? (a) IRR Program funds may be...

  14. 25 CFR 170.115 - What activities may be funded with IRR Program funds?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 1 2011-04-01 2011-04-01 false What activities may be funded with IRR Program funds? 170... RESERVATION ROADS PROGRAM Indian Reservation Roads Program Policy and Eligibility Eligible Uses If Irr Program Funds § 170.115 What activities may be funded with IRR Program funds? (a) IRR Program funds may be...

  15. Addressing Inclusion in Higher Education Physical Activity Programs

    ERIC Educational Resources Information Center

    Allar, Ishonté; Baek, Jun-Hyung; Taliaferro, Andrea

    2014-01-01

    The number of students with disabilities pursuing higher education has increased in the past decade. However, there is little information as to whether or not college basic instruction programs (CBIPs) are providing appropriate physical activity courses for individuals with disabilities. Those who teach CBIPs may have limited background or…

  16. Group Dynamics and Initiative Activities with Outdoor Programs.

    ERIC Educational Resources Information Center

    Zwaagstra, Lynn

    This paper focuses on group dynamics and introduces the use of initiative activities as a means of facilitating a more cohesive group experience in outdoor programs. Specific topics addressed and defined include: (1) curative factors of groups (universality, didactic learning, altruism, socialization, peer learning, group cohesiveness); (2) stages…

  17. 14 CFR 1253.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Education programs or activities. 1253.400 Section 1253.400 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION NONDISCRIMINATION ON...) Assistance administered by a recipient educational institution to study at a foreign institution. A...

  18. 14 CFR § 1253.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 5 2014-01-01 2014-01-01 false Education programs or activities. § 1253.400 Section § 1253.400 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION...) Assistance administered by a recipient educational institution to study at a foreign institution. A...

  19. 14 CFR 1253.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 5 2012-01-01 2012-01-01 false Education programs or activities. 1253.400 Section 1253.400 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION NONDISCRIMINATION ON...) Assistance administered by a recipient educational institution to study at a foreign institution. A...

  20. The Nature Specialist: A Complete Guide to Program and Activities.

    ERIC Educational Resources Information Center

    Miller, Lenore Hendler

    This manual contains a collection of approaches and activities for nature specialists at residential or day camps. It is organized so that the novice may use it to maximize the effectiveness of a camp program. The book is full of descriptions of various field experiments in which children can participate. Comments in section 1, "Getting Started,"…

  1. Aerobic Activity--Do Physical Education Programs Provide Enough?

    ERIC Educational Resources Information Center

    McGing, Eileen

    1989-01-01

    High school physical education curricula should concentrate less on sport skill development and competition, and more on health-related fitness and aerobic activity. Results are reported from a study of the type and amount of aerobic exercise provided in 29 high school physical education programs in a large metropolitan area. (IAH)

  2. Marketing Recreation and Physical Activity Programs for Females.

    ERIC Educational Resources Information Center

    Henderson, Karla A.

    1995-01-01

    Explores how marketing recreation and physical activity programs for females might be undertaken based on the growing body of research about female involvement in recreation, sport, and leisure. The paper addresses product, place, price, and promotion, discussing females as people who represent a growing market segment with unique characteristics.…

  3. 78 FR 41943 - Agency Information Collection Activities; Trusted Traveler Programs

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-12

    ... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities; Trusted Traveler Programs AGENCY: U.S. Customs and Border Protection, Department of Homeland Security. ACTION: 30-Day notice.... Customs and Border Protection (CBP) will be submitting the following information collection request to...

  4. 10 CFR 1042.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ....400 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex...) General. Except as provided elsewhere in these Title IX regulations, no person shall, on the basis of...

  5. Effectiveness of School-Initiated Physical Activity Program on Secondary School Students' Physical Activity Participation

    ERIC Educational Resources Information Center

    Gråstén, Arto; Yli-Piipari, Sami; Watt, Anthony; Jaakkola, Timo; Liukkonen, Jarmo

    2015-01-01

    Background: The promotion of physical activity and health has become a universal challenge. The Sotkamo Physical Activity as Civil Skill Program was implemented to increase students' physical activity by promoting supportive psychological and physical school environment. The aim of this study was to evaluate the effectiveness of the…

  6. Comprehensive School Physical Activity Programs: Helping All Students Achieve 60 Minutes of Physical Activity Each Day

    ERIC Educational Resources Information Center

    Elliot, Eloise; Erwin, Heather; Hall, Tina; Heidorn, Brent

    2013-01-01

    The American Alliance for Health, Physical Education, Recreation and Dance recommends that all schools implement a comprehensive school physical activity program. Physical activity is important to the overall health and well-being of everyone, including all school age children. The benefits of physical activity are well documented and include the…

  7. Community-Service Activities Versus Traditional Activities in an Intergenerational Visiting Program

    ERIC Educational Resources Information Center

    Marx, Marcia S.; Hubbard, Pamela; Cohen-Mansfield, Jiska; Dakheel-Ali, Maha; Thein, Khin

    2005-01-01

    The impact of traditional activities (e.g., playing board games) were compared with community-service activities (e.g., making first aid kits for a homeless shelter) during a monthly intergenerational visiting program. The participating seniors (n =19) gave high ratings to both types of activities. However, they felt that they had helped others…

  8. Elucidating Protactinium Hydrolysis: The Relative Stabilities of PaO2(H2O)(+) and PaO(OH)2(+).

    PubMed

    Dau, Phuong D; Wilson, Richard E; Gibson, John K

    2015-08-03

    It is demonstrated that the gas-phase oxo-exchange of PaO2(+) with water is substantially faster than that of UO2(+), indicating that the Pa-O bonds are more susceptible to activation and formation of the bis-hydroxide intermediate, PaO(OH)2(+). To elucidate the nature of the water adduct of PaO2(+), hydration of PaO2(+) and UO2(+), as well as collision induced dissociation (CID) and ligand-exchange of the water adducts of PaO2(+) and UO2(+), was studied. The results indicate that, in contrast to UO2(H2O)(+), the protactinium oxo bis-hydroxide isomer, PaO(OH)2(+), is produced as a gas-phase species close in energy to the hydrate isomer, PaO2(H2O)(+). CID behavior similar to that of Th(OH)3(+) supports the assignment as PaO(OH)2(+). The gas-phase results are consistent with the spontaneous hydrolysis of PaO2(+) in aqueous solution, this in contrast to later AnO2(+) (An = U, Np, Pu), which forms stable hydrates in both solution and gas phase. In view of the known propensity for Th(IV) to hydrolyze, and previous gas-phase studies of other AnO2(+), it is concluded that the stabilities of oxo-hydroxides relative to oxide hydrates decreases in the order: Th(IV) > Pa(V) > U(V) > Np(V) > Pu(V). This trend suggests increasing covalency and decreasing ionicity of An-O bonds upon proceeding across the actinide series.

  9. Nutrition Education--1973. Part 7--School Nutrition Education Programs. Hearings Held Pittsburgh, Pa., May 25, 1973. Hearings Before the Select Committee on Nutrition and Human Needs of the United States Senate, Ninety-third Congress, First Session.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. Senate Select Committee on Nutrition and Human Needs.

    The following witnesses testified before these hearings of the Select Committee on Nutrition and Human Needs: Mrs. G. Marsh, area consultant, Division of Food and Nutrition Services, Pennsylvania Department of Education; Mrs. G. Chegwidden, director, School Food Service, Franklin Regional School District, Murrysville, Pa.; Mr. S. Lympany, senior…

  10. Determining the reach of a home-based physical activity program for older adults within the context of a randomized controlled trial

    PubMed Central

    Harden, Samantha M.; Fanning, Jason T.; Motl, Robert W.; McAuley, Edward; Estabrooks, Paul A.

    2014-01-01

    Determining the reach of physical activity (PA) programs is challenging due to inconsistent reporting across studies. The purpose of this study was to document multiple indicators of program reach for a 6-month, Digital Versatile Disc (DVD)-delivered home-based PA program. Radio, newspaper and direct mailing advertisements were tracked to determine costs as well as the number and representativeness of older adults exposed and responding to recruitment. It was estimated that all older adults in the recruitment area (n = 105 515) may have been exposed to at least one of the recruitment strategies—563 responded and 383 were screened as eligible. Of those that enrolled (n = 307), the DVD reached between 81% and 97% of the participants over each month within the 6 month period. Newspaper advertisements were most effective (n = 222) at a cost of $78 per participant enrolled. Conclusion: Using multiple indicators of reach supports the accurate calculation and generalizability of recruiting older adults into PA programs. PMID:25122617

  11. Active Aging Promotion: Results from the Vital Aging Program

    PubMed Central

    Caprara, Mariagiovanna; Molina, María Ángeles; Schettini, Rocío; Santacreu, Marta; Orosa, Teresa; Mendoza-Núñez, Víctor Manuel; Rojas, Macarena; Fernández-Ballesteros, Rocío

    2013-01-01

    Active aging is one of the terms in the semantic network of aging well, together with others such as successful, productive, competent aging. All allude to the new paradigm in gerontology, whereby aging is considered from a positive perspective. Most authors in the field agree active aging is a multidimensional concept, embracing health, physical and cognitive fitness, positive affect and control, social relationships and engagement. This paper describes Vital Aging, an individual active aging promotion program implemented through three modalities: Life, Multimedia, and e-Learning. The program was developed on the basis of extensive evidence about individual determinants of active aging. The different versions of Vital Aging are described, and four evaluation studies (both formative and summative) are reported. Formative evaluation reflected participants' satisfaction and expected changes; summative evaluations yielded some quite encouraging results using quasi-experimental designs: those who took part in the programs increased their physical exercise, significantly improved their diet, reported better memory, had better emotional balance, and enjoyed more cultural, intellectual, affective, and social activities than they did before the course, thus increasing their social relationships. These results are discussed in the context of the common literature within the field and, also, taking into account the limitations of the evaluations accomplished. PMID:23476644

  12. Oxidation of Gas-Phase Protactinium Ions, Pa+ and Pa2+: Formation and Properties of PaO22+(g), Protactinyl

    SciTech Connect

    Santos, M.; de Matos, A. Pires; Marcalo, J.; Gibson, John K; Haire, Richard {Dick} G; Tyagi, R.; Pitzer, R. M.

    2006-01-01

    Oxidation reactions of bare and ligated, monopositive, and dipositive Pa ions in the gas phase were studied by Fourier transform ion cyclotron resonance mass spectrometry. Seven oxidants were employed, ranging from the thermodynamically robust N{sub 2}O to the relatively weak CH{sub 2}O - all oxidized Pa{sup +} to PaO{sup +} and PaO{sup +} to PaO{sub 2}{sup +}. On the basis of experimental observations, it was established that D[Pa{sup +}-O] and D[OPa{sup +}-O] {ge} 751 kJ mol{sup -1}. Estimates for D[Pa{sup +}-O], D[OPa{sup +}-O], IE[PaO], and IE[PaO{sub 2}] were also obtained. The seven oxidants reacted with Pa{sup 2+} to produce PaO{sup 2+}, indicating that D[Pa{sup 2+}-O] {ge} 751 kJ mol{sup -1}. A particularly notable finding was the oxidation of PaO{sup 2+} by N{sub 2}O to PaO{sub 2}{sup 2+}, a species, which formally comprises Pa(VI). Collision-induced dissociation of PaO{sub 2}{sup 2+} suggested the protactinyl connectivity, {l_brace}O-Pa-O{r_brace}{sup 2+}. The experimentally determined IE[PaO{sub 2}{sup +}] {approx} 16.6 eV is in agreement with self-consistent-field and configuration interaction calculations for PaO{sub 2}{sup +} and PaO{sub 2}{sup 2+}. These calculations provide insights into the electronic structures of these ions and indicate the participation of 5f orbitals in bonding and a partial '6p hole' in the case of protactinyl. It was found that PaO{sub 2}{sup 2+} catalyzes the oxidation of CO by N{sub 2}O - such O atom transport via a dipositive metal oxide ion is distinctive. It was also observed that PaO{sub 2}{sup 2+} is capable of activating H{sub 2} to form the stable PaO{sub 2}H{sup 2+} ion.

  13. National commercial solar heating and cooling demonstration: purposes, program activities, and implications for future programs

    SciTech Connect

    Koontz, R.; Genest, M.; Bryant, B.

    1980-05-01

    The Solar Heating and Cooling Demonstration Act of 1974 created a set of activities to demonstrate the potential use of solar heating within a three-year period and of combined solar heating and cooling within a five-year period. This study assesses the Commercial Demonstration Program portion of the activity in terms of its stated goals and objectives. The primary data base was DOE contractor reports on commercial demonstration projects. It was concluded that the program did not provide data to support a positive decision for the immediate construction or purchase of commercial solar systems. However, the program may have contributed to other goals in the subsequent legislation; i.e., research and development information, stimulation of the solar industry, and more informed policy decisions.

  14. Using active learning strategies to present bloodborne pathogen programs.

    PubMed

    Weaver, Mary G

    2003-06-01

    Every year, school nurses have the responsibility for developing and presenting a bloodborne pathogen presentation to the education and clerical staff of their buildings. Although the information is similar from year to year, the manner in which the information is presented can be altered. Teachers are using active learning strategies in a variety of learning environments, engaging students in the learning process by having them play an active role. With some planning, preparation, and imagination, active learning strategies can be incorporated into bloodborne pathogen presentations. The purpose of this article is to define active learning, describe how to develop a program using active learning strategies, and provide some examples of bloodborne pathogen presentations that have already been developed. Several sources are identified that can provide the school nurse with information regarding bloodborne pathogens. Information about how computers can be integrated into the bloodborne pathogen presentation is also presented.

  15. Current activities handbook: formerly utilized sites remedial action program

    SciTech Connect

    1981-02-27

    This volume is one of a series produced under contract with the DOE, by Politech Corporation to develop a legislative and regulatory data base to assist the FUSRAP management in addressing the institutional and socioeconomic issues involved in carrying out the Formerly Utilized Sites Remedial Action Program. This Information Handbook series contains information about all relevant government agencies at the Federal and state levels, the pertinent programs they administer, each affected state legislature, and current Federal and state legislative and regulatory initiatives. This volume is a compilation of information about the activities each of the thirteen state legislatures potentially affected by the Formerly Utilized Sites Remedial Action Program. It contains a description of the state legislative procedural rules and a schedule of each legislative session; a summary of pending relevant legislation; the name and telephone number of legislative and state agency contacts; and the full text of all bills identified.

  16. NASA safety program activities in support of the Space Exploration Initiatives Nuclear Propulsion program

    NASA Technical Reports Server (NTRS)

    Sawyer, J. C., Jr.

    1993-01-01

    The activities of the joint NASA/DOE/DOD Nuclear Propulsion Program Technical Panels have been used as the basis for the current development of safety policies and requirements for the Space Exploration Initiatives (SEI) Nuclear Propulsion Technology development program. The Safety Division of the NASA Office of Safety and Mission Quality has initiated efforts to develop policies for the safe use of nuclear propulsion in space through involvement in the joint agency Nuclear Safety Policy Working Group (NSPWG), encouraged expansion of the initial policy development into proposed programmatic requirements, and suggested further expansion into the overall risk assessment and risk management process for the NASA Exploration Program. Similar efforts are underway within the Department of Energy to ensure the safe development and testing of nuclear propulsion systems on Earth. This paper describes the NASA safety policy related to requirements for the design of systems that may operate where Earth re-entry is a possibility. The expected plan of action is to support and oversee activities related to the technology development of nuclear propulsion in space, and support the overall safety and risk management program being developed for the NASA Exploration Program.

  17. National Dam Inspection Program. Lower Hemlock Dam (NDI-ID Number PA-00756, DER-ID Number 52-117) Delaware River Basin, Pike County, Pennsylvania. Phase I Inspection Report.

    DTIC Science & Technology

    1980-06-01

    is covered with a large overhanging concrete slab and is accessible by a footbridge from the crest of the dam,(Photograph 5, Appendix C). Lower Hemlock...Valve house located in the reservoir accessible from the top of dam by concrete footbridge . B. OUTLET STRUCTURE 24" 0 concrete pipe discharging at...Concrete footbridge to intake. A-3 NDI NO. PA-O_ 756 VISUAL INSPECTION SPILLWAY OBSERVATIONS AND REMARKS A. APPROACH CHANNEL Approach is-directly from

  18. Motivation and Physical Activity Behaviors among Older Women: A Self-Determination Perspective

    ERIC Educational Resources Information Center

    Stephan, Yannick; Boiche, Julie; Le Scanff, Christine

    2010-01-01

    Drawing upon Self-Determination Theory, the purpose of our study was to examine the motivational determinants of older women's dropout and participation in physical activity (PA). Older women who dropped out (n = 242) or remained (n = 332) in an organized PA program completed the Sport Motivation Scale as well as health and PA measures. We found…

  19. The CELSS research program - A brief review of recent activities

    NASA Technical Reports Server (NTRS)

    Macelroy, R. D.; Tremor, J.; Bubenheim, D. L.; Gale, J.

    1989-01-01

    The history of the Controlled Ecological Life Support System program, initiated by NASA in the late 1970s to explore the use of bioregenerative methods of life support, is reviewed. The project focused on examining the process involved in converting inorganic minerals and gases into life support materials using sunlight as the primary energy source. The research, planning, and technological development required by the CELSS program and conducted at NASA field centers, at various universities, and by commercial organizations are reviewed. Research activities at universities have focused upon exploring methods of reducing the size of the system, reducing system power requirements, understanding issues that are associated with its long-term stability, and identifying new technologies that might be useful in improving its efficiency. Research activities at Ames research center have focused on the use of common duckweed as a high biomass-producing plant, which is high in protein and on waste processing.

  20. 25 CFR 170.116 - What activities are not eligible for IRR Program funding?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 1 2011-04-01 2011-04-01 false What activities are not eligible for IRR Program funding... Irr Program Funds § 170.116 What activities are not eligible for IRR Program funding? IRR Program... programs and projects; (e) Work performed on projects that are not included on an FHWA-approved...

  1. 25 CFR 170.116 - What activities are not eligible for IRR Program funding?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false What activities are not eligible for IRR Program funding... Irr Program Funds § 170.116 What activities are not eligible for IRR Program funding? IRR Program... programs and projects; (e) Work performed on projects that are not included on an FHWA-approved...

  2. United States Department of Energy Thermally Activated Heat Pump Program

    SciTech Connect

    Fiskum, R.J.; Adcock, P.W.; DeVault, R.C.

    1996-06-01

    The US Department of Energy (DOE) is working with partners from the gas heating and cooling industry to improve energy efficiency using advance absorption technologies, to eliminate chlorofluorocarbons (CFCs) and hydrochlorofluorocarbons (HCFCs), to reduce global warming through more efficient combustion of natural gas, and to impact electric peak demand of air conditioning. To assist industry in developing these gas heating and cooling absorption technologies, the US DOE sponsors the Thermally Activated Heat Pump Program. It is divided into five key activities, addressing residential gas absorption heat pumps, large commercial chillers, advanced absorption fluids, computer-aided design, and advanced ``Hi-Cool`` heat pumps.

  3. Geodetic activities of the Department of Defense under IGY programs

    SciTech Connect

    Williams, O.W.; Daugherty, K.I.

    1983-10-16

    Attention is given to the U.S. Department of Defence (DOD) activities that contributed to the International Geophysical Year's active, passive, and cooperative satellite programs. The DOD continues to support the deployment, enhancement, and application of novel technology in such areas as satellite altimetry, gravity radiometry, inertial surveying, interferometry, airborne gravimetry, inertial surveying, and CCD and laser methods for geodetic astronomy. Also noted are such major department initiatives as the Global Positioning System, which will become operational toward the end of this decade.

  4. Microfluidic manufacture of rt-PA-loaded echogenic liposomes

    PubMed Central

    Kandadai, Madhuvanthi A.; Mukherjee, Prithviraj; Shekhar, Himanshu; Shaw, George J.; Papautsky, Ian; Holland, Christy K.

    2016-01-01

    Echogenic liposomes (ELIP), loaded with recombinant tissue-type plasminogen activator (rt-PA) and microbubbles that act as cavitation nuclei, are under development for ultrasound-mediated thrombolysis. Conventional manufacturing techniques produce a polydisperse rt-PA-loaded ELIP population with only a small percentage of particles containing microbubbles. Further, a polydisperse population of rt-PA-loaded ELIP has a broadband frequency response with complex bubble dynamics when exposed to pulsed ultrasound. In this work, a microfluidic flow-focusing device was used to generate monodisperse rt-PA-loaded ELIP (µtELIP) loaded with a perfluorocarbon gas. The rt-PA associated with the µtELIP was encapsulated within the lipid shell as well as intercalated within the lipid shell. The µtELIP had a mean diameter of 5 µm, a resonance frequency of 2.2 MHz, and were found to be stable for at least 30 min in 0.5%bovine serum albumin. Additionally, 35 % of µtELIP particles were estimated to contain microbubbles, an order of magnitude higher than that reported previously for batch-produced rt-PA-loaded ELIP. These findings emphasize the advantages offered by microfluidic techniques for improving the encapsulation efficiency of both rt-PA and perflurocarbon microbubbles within echogenic liposomes. PMID:27206512

  5. CDC's Emergency Management Program activities - worldwide, 2003-2012.

    PubMed

    2013-09-06

    In 2003, recognizing the increasing frequency and complexity of disease outbreaks and disasters and a greater risk for terrorism, CDC established the Emergency Operations Center (EOC), bringing together CDC staff members who respond to public health emergencies to enhance communication and coordination. To complement the physical EOC environment, CDC implemented the Incident Management System (IMS), a staffing structure and set of standard operational protocols and services to support and monitor CDC program-led responses to complex public health emergencies. The EOC and IMS are key components of CDC's Emergency Management Program (EMP), which applies emergency management principles to public health practice. To enumerate activities conducted by the EMP during 2003-2012, CDC analyzed data from daily reports and activity logs. The results of this analysis determined that, during 2003-2012, the EMP fully activated the EOC and IMS on 55 occasions to support responses to infectious disease outbreaks, natural disasters, national security events (e.g., conventions, presidential addresses, and international summits), mass gatherings (e.g., large sports and social events), and man-made disasters. On 109 other occasions, the EMP was used to support emergency responses that did not require full EOC activation, and the EMP also conducted 30 exercises and drills. This report provides an overview of those 194 EMP activities.

  6. Impact of a walking school bus program on children's active commuting to school and physical activity

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Walking School Buses (WSB) are groups of students, led to and from school by adults, in which students are picked up at designated "bus stops". The impact of WSB programs on students' active commuting to school (walking/cycling to school, ACS), and moderate-to vigorous-physical activity (MVPA) has n...

  7. Crystal Structures of An F420-Dependent Glucose-6-Phosphate Dehydrogenase Fgd1 Involved in the Activation of the Anti-Tb Drug Candidate Pa-824 Reveal the Basis of Coenzyme And Substrate Binding

    SciTech Connect

    Bashiri, G.; Squire, C.J.; Moreland, N.J.; Baker, E.N.

    2009-05-11

    The modified flavin coenzyme F{sub 420} is found in a restricted number of microorganisms. It is widely distributed in mycobacteria, however, where it is important in energy metabolism, and in Mycobacterium tuberculosis (Mtb) is implicated in redox processes related to non-replicating persistence. In Mtb, the F{sub 420}-dependent glucose-6-phosphate dehydrogenase FGD1 provides reduced F{sub 420} for the in vivo activation of the nitroimidazopyran prodrug PA-824, currently being developed for anti-tuberculosis therapy against both replicating and persistent bacteria. The structure of M. tuberculosis FGD1 has been determined by x-ray crystallography both in its apo state and in complex with F{sub 420} and citrate at resolutions of 1.90 and 1.95{angstrom}, respectively. The structure reveals a highly specific F{sub 420} binding mode, which is shared with several other F{sub 420}-dependent enzymes. Citrate occupies the substrate binding pocket adjacent to F{sub 420} and is shown to be a competitive inhibitor (IC{sub 50} 43 {micro}m). Modeling of the binding of the glucose 6-phosphate (G6P) substrate identifies a positively charged phosphate binding pocket and shows that G6P, like citrate, packs against the isoalloxazine moiety of F{sub 420} and helps promote a butterfly bend conformation that facilitates F{sub 420} reduction and catalysis.

  8. National Dam Inspection Program. Kunkles Dam (NDS I.D. Number PA 00669, DER I.D. Number 54-67), Delaware River Basin, Pine Creek, Schuylkill County, Pennsylvania. Phase I Inspection Report.

    DTIC Science & Technology

    1980-06-01

    APPROVED BY: Date 48 4 04 ivi TABLE OF CONTENTS PAGE Preface i Assessment and Recommendations ii Overview Photograph iv SECTION 1- PROJECT INFORMATION 1.1...is performed. Included in this Appendix are the HEC-l, Rev. pertinent input values and a summary print-out tables . sV 󈧔’.1.. oA r azj/.3...3. 3. 3. 3. 3. 2. 2. 2. 2. 0 ENP-OF- PERID FLOV 40.0A HR.MN PERIOD RAIN EXES LOSS COMP 1t HO.PA 4R.Mo PERIOD RAIN (XCS LOSS COP SUM 6.54 0.42 .12

  9. National Dam Inspection Program. Flat Rock Dam (NDS I.D. Number PA 00896, DER I.D. Number 51-1), Delaware River Basin, Schuylkill River, Montgomery and Philadelphia Counties, Pennsylvania. Phase I Inspection Report,

    DTIC Science & Technology

    1980-08-01

    engineering characteristics of the dam include the Water Resources Bulletin, Bulletin No. 4, "Water Resources Survey, The Schuylkill River , Pennsylvania...2.8 12.5 iii,,1.0 .0 12 .0*2 1111 L A, 1 .2 1 1 MICROCOPY RESOLUTION TEST CHART 10AIIONA1 0111I AAil 0 IANDAR[1, 101,3 A DELAWARE RIVER BA(IN SNOYLKILL... RIVER UIUTllUERY AND PUILADELPEIA eoNuTIES PENNSYLVANIA lOS I PA. 06816 DER IB 51-1 . t0 II PHASE I INSPECTION REPORT I NATIONAL DAM INSPECTION

  10. Active Sites Environmental Monitoring Program: Mid-FY 1991 report

    SciTech Connect

    Ashwood, T.L.; Wickliff, D.S.; Morrissey, C.M.

    1991-10-01

    This report summarizes the activities of the Active Sites Environmental Monitoring Program (ASEMP) from October 1990 through March 1991. The ASEMP was established in 1989 by Solid Waste Operations and the Environmental Sciences Division to provide early detection and performance monitoring at active low-level radioactive waste (LLW) disposal sites in Solid Waste Storage Area (SWSA) 6 and transuranic (TRU) waste storage sites in SWSA 5 as required by chapters II and III of US Department of Energy Order 5820.2A. Monitoring results continue to demonstrate the no LLW is being leached from the storage vaults on the tumulus pads. Loading of vaults on Tumulus II began during this reporting period and 115 vaults had been loaded by the end of March 1991.

  11. Active objects programming for military autonomous mobile robots software prototyping

    NASA Astrophysics Data System (ADS)

    Cozien, Roger F.

    2001-09-01

    While designing mobile robots, we do think that the prototyping phase is really critical. Good and clever choices have to be made. Indeed, we may not easily upgrade such robots, and most of all, when the robot is on its own, any change in both the software and the physical body is going to be very difficult, if not impossible. Thus, a great effort has to be made when prototyping the robot. Furthermore, I think that the kind of programming is very important. If your programming model is not expressive enough, you may experience a great deal of difficulties to add all the features you want, in order to give your robot reactiveness and decision making autonomy. Moreover, designing, and prototyping the on-board software of a reactive robot brings other difficulties. A reactive robot does not include any matter of rapidity. A reactive system is a system able to respond to a huge pannel of situations of which it does not have the schedule. In other words, for instance, the robot does not know when a particular situation may occur, and overall, what it would be doing at this time, and what would be its internal state. This kind of robot must be able to take a decision and to act even if they do not have all the contextual information. To do so, we use a computer language named oRis featuring object and active object oriented programming, but also parallel and dynamic code, (the code can be changed during its own execution). This last point has been made possible because oRis is fully interpreted. However oRis may call fully compiled code, but also Prolog and Java code. An oRis program may be distributed on several computers using TCP/IP network connections. The main issue in this paper is to show how active objet oriented programming, as a modern extension of object oriented programming, may help us in designing autonomous mobile robots. Based on a fully parallel software programming, an active object code allows us to give many features to a robot, and to easily solve

  12. Active object programming for military autonomous mobile robot software prototyping

    NASA Astrophysics Data System (ADS)

    Cozien, Roger F.

    2001-10-01

    While designing mobile robots, we do think that the prototyping phase is really critical. Good and clever choices have to be made. Indeed, we may not easily upgrade such robots, and most of all, when the robot is on its own, any change in both the software and the physical body is going to be very difficult, if not impossible. Thus, a great effort has to be made when prototyping the robot. Furthermore, I think that the kind of programming is very important. If your programming model is not expressive enough, you may experience a great deal of difficulties to add all the features you want, in order to give your robot reactiveness and decision making autonomy. Moreover, designing, and prototyping the on-board software of a reactive robot brings other difficulties. A reactive robot does not include any matter of rapidity. A reactive system is a system able to respond to a huge panel of situations of which it does not have the schedule. In other words, for instance, the robot does not know when a particular situation may occur, and overall, what it would be doing at this time, and what would be its internal state. This kind of robot must be able to take a decision and to act even if they do not have all the contextual information. To do so, we use a computer language named oRis featuring object and active object oriented programming, but also parallel and dynamic code, (the code can be changed during its own execution). This last point has been made possible because oRis is fully interpreted. However oRis may call fully compiled code, but also Prolog and Java code. An oRis program may be distributed on several computers using TCP/IP network connections. The main issue in this paper is to show how active objet oriented programming, as a modern extension of object oriented programming, may help us in designing autonomous mobile robots. Based on a fully parallel software programming, an active object code allows us to give many features to a robot, and to easily solve

  13. An introprofessional geriatric medication activity within a senior mentor program.

    PubMed

    Shrader, Sarah; Hummel, Heather; Byrd, Lauren; Wiley, Kathy

    2013-02-12

    Objective. To determine whether a required interprofessional geriatric medication activity within a senior mentor program changed pharmacy and medical students' attitudes regarding interprofessional collaboration.Design. Interprofessional teams, consisting of 1 third-year pharmacy student and 2 second-year medical students, conducted an in-home interview and medication history with a senior mentor (geriatric patient). The team members then collaboratively analyzed and discussed the patient's medication use and wrote an essay in which they identified the patient's medication problems and reflected on the interprofessional experience.Assessment. Students completed a validated survey instrument to measure pharmacist-physician attitudes about interprofessional collaboration before and after the experience. Pharmacy and medical students' already generally positive attitudes regarding interprofessional relationships were maintained and, in some instances, significantly improved. Students found the activity enhanced their geriatric training and increased their understanding of an interprofessional team.Conclusion. Incorporation of a geriatric medication activity within a senior mentor program maintained or improved pharmacy and medical students' positive attitudes about interprofessional collaboration and enhanced geriatric training within the curriculum.

  14. Crystal structure of the flavoenzyme PA4991 from Pseudomonas aeruginosa

    SciTech Connect

    Jacewicz, Agata; Schnell, Robert; Lindqvist, Ylva; Schneider, Gunter

    2016-01-22

    PA4991 is a FAD-dependent oxidoreductase from the pathogen P. aeruginosa that is essential for virulence and survival in the infected host. The structure of this enzyme, determined to 2.4 Å resolution, reveals that PA4991 belongs to the GR{sub 2} family of flavoenzymes. The locus PA4991 in Pseudomonas aeruginosa encodes an open reading frame that has been identified as essential for the virulence and/or survival of this pathogenic organism in the infected host. Here, it is shown that this gene encodes a monomeric FAD-binding protein of molecular mass 42.2 kDa. The structure of PA4991 was determined by a combination of molecular replacement using a search model generated with Rosetta and phase improvement by a low-occupancy heavy-metal derivative. PA4991 belongs to the GR{sub 2} family of FAD-dependent oxidoreductases, comprising an FAD-binding domain typical of the glutathione reductase family and a second domain dominated by an eight-stranded mixed β-sheet. Most of the protein–FAD interactions are via the FAD-binding domain, but the isoalloxazine ring is located at the domain interface and interacts with residues from both domains. A comparison with the structurally related glycine oxidase and glycerol-3-phosphate dehydrogenase shows that in spite of very low amino-acid sequence identity (<18%) several active-site residues involved in substrate binding in these enzymes are conserved in PA4991. However, enzymatic assays show that PA4991 does not display amino-acid oxidase or glycerol-3-phosphate dehydrogenase activities, suggesting that it requires different substrates for activity.

  15. Impact of an activities-based adult dementia care program

    PubMed Central

    Higgins, Margaret; Koch, Kathleen; Hynan, Linda S; Carr, Sandra; Byrnes, Kathleen; Weiner, Myron F

    2005-01-01

    The investigators studied over one year the impact of a newly established once-a-week activity-based day care program for dementia patients combined with 17 educational sessions for caregivers held at the same facility. Outcome measures were patient and caregiver quality of life (QOL), patient behavioral disturbance, and use of community-based resources. Of the 37 enrollees, 3 chose not to start the program and 13 dropped out before the end of one year, largely due to health-related issues. Of the initial group, 21 attended for the entire year. The average patient Mini-Mental State Exam (MMSE) score at entry was 16, indicating a moderate level of dementia. Average score on the CERAD Behavior Rating Scale for Dementia (BRSD) was 30.1, indicating a mild level of behavioral disturbance. Attendance at day care was 91%; at the caregiver educational sessions, 74%. Patient and caregiver enthusiasm for the program was high and all wanted to continue attendance beyond the study period despite the fact that patients reported no change in QOL. Caregivers rated patients as having significantly less QOL, and rated their own QOL as unchanged. Symptomatic patient behaviors, as measured by the BRSD, increased significantly over the period of study. Caregivers reported greater use of community resources. PMID:18568062

  16. Cumberlandian Mollusk Conservation Program. Activity 1: mussel distribution surveys

    SciTech Connect

    Ahlstedt, S.A.

    1986-01-01

    The distribution of Cumberlandian mollusks in the Tennessee Valley is one of nine research activities developed as part of TVA's Cumberlandian Mollusk Conservation Program (CMCP). The name Cumberlandian refers to an endemic faunal assemblage that encompasses portions of 7 states bordering the southern Appalachian Mountains and the Cumberland Plateau Region. This geographic region is known as one of the major centers for mussel speciation and is considered the most prolific areas of the world for this particular group of organisms. Nine Tennessee Valley streams were selected for intensive qualitative and quantitative mussel surveys under Activity I of the CMCP. The surveys were designed to gather information on the present distribution of Cumberlandian mollusks. The streams chosen for surveys were based on the documented presence of diverse mussel fauna, endangered mussels, and/or sufficient information (diverse fish fauna, good water quality, etc.) to suggest potential for occurrence of diverse mussel fauna or endangered species.

  17. [Co-integration of BLG-LAtPA and WAP improved the expression of LAtPA in transgenic mouse milk].

    PubMed

    Zhou, J; Deng, J X; Cheng, X; Lu, Y F; Yang, X; Huang, P T

    2001-01-01

    In order to improve the expression of longer acting tissue plasminogen activator in the mammary epithelium of transgenic mice, the fragment of BLG-LAtPA hydrid gene was microinjected into mouse embryos with mice whey acid protein gene. Three mouse were tested as being Co-integration of BLG-LAtPA and WAP transgene by PCR and Southern blot. Milk obtained from lactating females contains biologically active tPA, and the concentration of tPA was calculated to be about 10 micrograms/mL.

  18. The Pediocin PA-1 Accessory Protein Ensures Correct Disulfide Bond Formation in the Antimicrobial Peptide Pediocin PA-1.

    PubMed

    Oppegård, Camilla; Fimland, Gunnar; Anonsen, Jan Haug; Nissen-Meyer, Jon

    2015-05-19

    Peptides, in contrast to proteins, are generally not large enough to form stable and well-defined three-dimensional structures. However, peptides are still able to form correct disulfide bonds. Using pediocin-like bacteriocins, we have examined how this may be achieved. Some pediocin-like bacteriocins, such as pediocin PA-1 and sakacin P[N24C+44C], have four cysteines. There are three possible ways by which the four cysteines may combine to form two disulfide bonds, and the three variants are expected to be produced in approximately equal amounts if their formation is random. Pediocin PA-1 and sakacin P[N24C+44C] with correct disulfide bonds were the main products when they were secreted by the pediocin PA-1 ABC transporter and accessory protein, but when they were secreted by the corresponding secretion machinery for sakacin A, a pediocin-like bacteriocin with one disulfide bond (two cysteines), peptides with all three possible disulfide bonds were produced in approximately equal amounts. All five cysteines in the pediocin PA-1 ABC transporter and the two cysteines (that form a CxxC motif) in the accessory protein were individually replaced with serines to examine their involvement in disulfide bond formation in pediocin PA-1. The Cys86Ser mutation in the accessory protein caused a 2-fold decrease in the amount of pediocin PA-1 with correct disulfide bonds, while the Cys83Ser mutation nearly abolished the production of pediocin PA-1 and resulted in the production of all three disufide bond variants in equal amounts. The Cys19Ser mutation in the ABC transporter completely abolished secretion of pediocin PA-1, suggesting that Cys19 is in the proteolytic active site and involved in cleaving the prebacteriocin. Replacing the other four cysteines in the ABC transporter with serines caused a slight reduction in the overall amount of secreted pediocin PA-1, but the relative amount with the correct disulfide bonds remained large. These results indicate that the pediocin

  19. LABORATORY DIRECTED RESEARCH AND DEVELOPMENT PROGRAM ACTIVITIES FOR FY2002.

    SciTech Connect

    FOX,K.J.

    2002-12-31

    and a means to address national needs within the overall mission of the DOE and BNL. The LDRD Annual Report contains summaries of all research activities funded during Fiscal Year 2002. The Project Summaries with their accomplishments described in this report reflect the above. Aside from leading to new fundable or promising programs and producing especially noteworthy research, the LDRD activities have resulted in numerous publications in various professional and scientific journals and presentations at meetings and forums. All Fy 2002 projects are listed and tabulated in the Project Funding Table. Also included in this Annual Report in Appendix A is a summary of the proposed projects for FY 2003. The BNL LDRD budget authority by DOE in FY 2002 was $7 million. The actual allocation totaled $6.7 million. The following sections in this report contain the management processes, peer review, and the portfolio's relatedness to BNL's mission, initiatives and strategic plans. Also included is a metric of success indicators.

  20. Crystal structure of the flavoenzyme PA4991 from Pseudomonas aeruginosa

    PubMed Central

    Jacewicz, Agata; Schnell, Robert; Lindqvist, Ylva; Schneider, Gunter

    2016-01-01

    The locus PA4991 in Pseudomonas aeruginosa encodes an open reading frame that has been identified as essential for the virulence and/or survival of this pathogenic organism in the infected host. Here, it is shown that this gene encodes a monomeric FAD-binding protein of molecular mass 42.2 kDa. The structure of PA4991 was determined by a combination of molecular replacement using a search model generated with Rosetta and phase improvement by a low-occupancy heavy-metal derivative. PA4991 belongs to the GR2 family of FAD-dependent oxidoreductases, comprising an FAD-binding domain typical of the glutathione reductase family and a second domain dominated by an eight-stranded mixed β-sheet. Most of the protein–FAD interactions are via the FAD-binding domain, but the isoalloxazine ring is located at the domain interface and interacts with residues from both domains. A comparison with the structurally related glycine oxidase and glycerol-3-phosphate dehydrogenase shows that in spite of very low amino-acid sequence identity (<18%) several active-site residues involved in substrate binding in these enzymes are conserved in PA4991. However, enzymatic assays show that PA4991 does not display amino-acid oxidase or glycerol-3-phosphate dehydrogenase activities, suggesting that it requires different substrates for activity. PMID:26841760

  1. Integrating health education and physical activity programming for cardiovascular health promotion among female inmates: A proof of concept study.

    PubMed

    Nair, Uma S; Jordan, Jeremy S; Funk, Daniel; Gavin, Kristin; Tibbetts, Erica; Collins, Bradley N

    2016-05-01

    Female inmate populations in the United States tend to be overweight, physically inactive, experience high stress, and have a history of nicotine and other drug dependence. Thus, they bear an elevated risk of cardiovascular (CV) disease than the general population. However, few evidence-based health interventions exist for this population. This study will test proof of concept, feasibility, and potential efficacy of a multiple health behavior change intervention that integrates CV-health promotion education delivered during a physical activity (PA) program (indoor cycling) tailored to this population. This study uses a quasi-experimental 2-group design with two measurement time-points: baseline and 8-week end of treatment. N=120 incarcerated women (18-59years of age) who are medically cleared for participation in PA will be enrolled. Indoor cycling instructors will be trained to deliver five health education topics over an 8-week period during twice-weekly cycling classes. Topics match the American Heart Association recommendations for CV health: (a) nutrition, (b) PA promotion, (c) weight management, (d) stress management, and (e) smoking cessation and relapse prevention. Modes of intervention include instructor advice, written materials and audio/video clips reviewed during class. CV-related and mental health measures will be assessed at both time-points. Results will guide a full scale efficacy study. Future research in this area has potential to impact the health of female inmates, a high-risk population. Moreover, this multiple health behavior change intervention model represents a community approach to health promotion that could generalize to other underserved populations who may benefit most from similar intervention efforts.

  2. Promoting Active Learning: The Use of Computational Software Programs

    NASA Astrophysics Data System (ADS)

    Dickinson, Tom

    The increased emphasis on active learning in essentially all disciplines is proving beneficial in terms of a student's depth of learning, retention, and completion of challenging courses. Formats labeled flipped, hybrid and blended facilitate face-to-face active learning. To be effective, students need to absorb a significant fraction of the course material prior to class, e.g., using online lectures and reading assignments. Getting students to assimilate and at least partially understand this material prior to class can be extremely difficult. As an aid to achieving this preparation as well as enhancing depth of understanding, we find the use of software programs such as Mathematica®or MatLab®, very helpful. We have written several Mathematica®applications and student exercises for use in a blended format two semester E&M course. Formats include tutorials, simulations, graded and non-graded quizzes, walk-through problems, exploration and interpretation exercises, and numerical solutions of complex problems. A good portion of this activity involves student-written code. We will discuss the efficacy of these applications, their role in promoting active learning, and the range of possible uses of this basic scheme in other classes.

  3. Psychological Well-Being (PWB) of School Adolescents Aged 12–18 yr, its Correlation with General Levels of Physical Activity (PA) and Socio-Demographic Factors In Gilgit, Pakistan

    PubMed Central

    KHAN, Yasmin; TAGHDISI, Muhammad Hussain; NOURIJELYANI, Keramat

    2015-01-01

    Background: Adolescence is a critical developmental stage marked by complex transitions. The purpose of study was to assess school adolescents’ PWB, examine the relationship of PA and socio-demographic factors with PWB. Methods: A cross sectional study conducted in five randomly selected schools with 345 adolescents (aged 12–18) from grade 6th–10th. A self-administered Well-Being index was adapted to measure PWB and questionnaire for adolescents PA (PAQ-A). Socio-demographic variables determined: age, gender, household income and parental education. Bivariate and multivariate regression analyses performed to examine the association between PWB, PA and covariates. Results: Findings indicated the mean age 14.64 (SD=1.275), 55.4% were female. Without gender difference the majority (43.4%) of adolescents showed moderate, while (23.2%) revealed low level of PWB. Participants with low level likely to have depression but scores were significantly not different between low, moderate and high PWB with PA. Socio-demographic trends of adolescents’ were observed significant (P <0.005) for PWB. In multivariable analysis the mean wellbeing in females adjusted for other covariates was significant (P =0.004) than males. PWB importantly (P <0.001) decreased by 3.36 units as its covariates increased and PA score in boys found 0.05 unit more than girls. Conclusion: The study results are invaluable in addressing low, moderate and high levels of PWB. Inadequate PA and PWB decreasing with some socio-demographic covariates is crucial health issue among female adolescents in Pakistan. Further studies need to find barrier, social indicators of PWB and implication of health among adolescents. PMID:26258093

  4. EDITORIAL: Adaptive and active materials: Selected papers from the ASME 2010 Conference on Smart Materials, Adaptive Structures and Intelligent Systems (SMASIS 10) (Philadelphia, PA, USA, 28 September-1 October 2010) Adaptive and active materials: Selected papers from the ASME 2010 Conference on Smart Materials, Adaptive Structures and Intelligent Systems (SMASIS 10) (Philadelphia, PA, USA, 28 September-1 October 2010)

    NASA Astrophysics Data System (ADS)

    Brei, Diann

    2011-09-01

    The third annual meeting of the AMSE/AIAA Smart Materials, Adaptive Structures and Intelligent Systems Conference (SMASIS) took place in the heart of historic Philadelphia's cultural district, and included a pioneer banquet in the National Constitutional Center. The applications emphasis of the 2010 conference was reflected in keynote talks by Dr Alan Taub, vice president of General Motors global research and development, 'Smart materials in the automotive industry'; Dr Charles R Farrar, engineering institute leader at Los Alamos National Laboratory, 'Future directions for structural health monitoring of civil engineering infrastructure'; and Professor Christopher S Lynch of the University of California Los Angeles, 'Ferroelectric materials and their applications'. The SMASIS conference was divided into six technical symposia each of which included basic research, applied technological design and development, and industrial and governmental integrated system and application demonstrations. The six symposia were: SYMP 1 Multifunctional Materials; SYMP 2 Active Materials, Mechanics and Behavior; SYMP 3 Modeling, Simulation and Control; SYMP 4 Enabling Technologies and Integrated System Design; SYMP 5 Structural Health Monitoring/NDE; and SYMP 6 Bio-inspired Smart Materials and Structures. In addition, the conference introduced a new student and young professional development symposium. Authors of papers in the materials areas (symposia 1, 2 and 6) were invited to write a full journal article on their presentation topic for publication in this special issue of Smart Materials and Structures. This set of papers demonstrates the exceptional quality and originality of the conference presentations. We are appreciative of their efforts in producing this collection of highly relevant articles on smart materials.

  5. Comprehensive School-Based Physical Activity Promotion: A Review

    ERIC Educational Resources Information Center

    Erwin, Heather; Beighle, Aaron; Carson, Russell L.; Castelli, Darla M.

    2013-01-01

    Physical activity (PA) participation levels among youth remain well below national recommendations. Thus, a variety of strategies to promote youth PA have been advocated, including multifaceted, school-based approaches. One identified as having great potential is a comprehensive school physical activity program (CSPAP). The goal of a CSPAP is to…

  6. Perspectives on Physical Activity and Exercise Among Appalachian Youth

    PubMed Central

    Swanson, Mark; Schoenberg, Nancy E.; Erwin, Heather; Davis, Rian E.

    2015-01-01

    Background Most children in the United States receive far less physical activity (PA) than is optimal. In rural, under resourced areas of Appalachian Kentucky, physical inactivity rates are significantly higher than national levels. We sought to understand children’s perceptions of PA, with the goal of developing culturally appropriate programming to increase PA. Methods During 11 focus groups, we explored perspectives on PA among 63 Appalachian children, ages 8–17. Sessions were tape recorded, transcribed, content analyzed, and subjected to verification procedures. Results Several perspectives on PA emerged among these rural Appalachian youth, including the clear distinction between PA (viewed as positive) and exercise (viewed as negative) and an emphasis on time and resource factors as barriers to adequate PA. Additional PA determinants expressed in the focus groups are similar to those of other populations. We include children’s recommendations for appealing PA programs. Conclusions Appalachian and other rural residents contend with the loss of rural health advantages (due to declines in farming/other occupational and avocational transitions). At the same time, Appalachian residents have not benefitted from urban PA facilitators (sidewalks, recreational facilities, clubs and organized leisure activities). Addressing low PA levels requires extensive community input and creative programming. PMID:22397810

  7. 20 CFR 632.173 - Allowable program activities.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... NATIVE AMERICAN EMPLOYMENT AND TRAINING PROGRAMS Job Training Partnership Act Programs Under Title IV... costs associated with special training projects where it is clear the benefits support the...

  8. Building a foundation for systems change: increasing access to physical activity programs for older adults.

    PubMed

    Lachenmayr, Sue; Mackenzie, Geraldine

    2004-10-01

    Although 25% of U.S. adults are physically inactive, this percentage increases dramatically for older adults. Organizational change theory guided a state health department in identifying system gaps and developing strategies to expand programming for seniors. A survey of provider agencies in New Jersey assessed (a) capacity for physical activity programs for older adults, (b) accessibility of programs, and (c) barriers to providing programs. One hundred sixty agencies provided physical activity programs to almost 184,000 individuals annually. Fewer than one half of the agencies provided exercise programs for people with disabilities, and only 44% provided in-home programs. Eighty-two percent of program providers wanted to expand programming but cited lack of trained instructors and peer leaders, inadequate facility space, insufficient funding, and limited transportation resources as barriers. Sustaining older adult behavior change requires infrastructure that will ensure access to diverse physical activities. This article provides strategies to expand access to physical activity programs for older adults.

  9. Laboratory Directed Research and Development Program Activities for FY 2008.

    SciTech Connect

    Looney,J.P.; Fox, K.

    2009-04-01

    with limited management filtering to encourage the creativity of individual researchers. The competition is open to all BNL staff in programmatic, scientific, engineering, and technical support areas. Researchers submit their project proposals to the Assistant Laboratory Director for Policy and Strategic Planning. A portion of the LDRD budget is held for the Strategic LDRD (S-LDRD) category. Projects in this category focus on innovative R&D activities that support the strategic agenda of the Laboratory. The Laboratory Director entertains requests or articulates the need for S-LDRD funds at any time. Strategic LDRD Proposals also undergo rigorous peer review; the approach to review is tailored to the size and scope of the proposal. These Projects are driven by special opportunities, including: (1) Research project(s) in support of Laboratory strategic initiatives as defined and articulated by the Director; (2) Research project(s) in support of a Laboratory strategic hire; (3) Evolution of Program Development activities into research and development activities; and (4) ALD proposal(s) to the Director to support unique research opportunities. The goals and objectives of BNL's LDRD Program can be inferred fronl the Program's stated purposes. These are to (1) encourage and support the development of new ideas and technology, (2) promote the early exploration and exploitation of creative and innovative concepts, and (3) develop new 'fundable' R&D projects and programs. The emphasis is clearly articulated by BNL to be on supporting exploratory research 'which could lead to new programs, projects, and directions' for the Laboratory. We explicitly indicate that research conducted under the LDRD Program should be highly innovative, and an element of high risk as to success is acceptable. To be one of the premier DOE National Laboratories, BNL must continuously foster groundbreaking scientific research. At Brookhaven National Laboratory one such method is through its LDRD Program

  10. Efficacy of histotripsy combined with rt-PA in vitro

    NASA Astrophysics Data System (ADS)

    Bader, Kenneth B.; Haworth, Kevin J.; Shekhar, Himanshu; Maxwell, Adam D.; Peng, Tao; McPherson, David D.; Holland, Christy K.

    2016-07-01

    Histotripsy, a form of therapeutic ultrasound that uses the mechanical action of microbubble clouds for tissue ablation, is under development to treat chronic deep vein thrombosis (DVT). We hypothesize that combining thrombolytic agents with histotripsy will enhance clot lysis. Recombinant tissue plasminogen activator (rt-PA) and rt-PA-loaded echogenic liposomes that entrain octafluoropropane microbubbles (OFP t-ELIP) were used in combination with highly shocked histotripsy pulses. Fully retracted porcine venous clots, with similar features of DVT occlusions, were exposed either to histotripsy pulses alone (peak negative pressures of 7-20 MPa), histotripsy and OFP t-ELIP, or histotripsy and rt-PA. Microbubble cloud activity was monitored with passive cavitation imaging during histotripsy exposure. The power levels of cavitation emissions from within the clot were not statistically different between treatment types, likely due to the near instantaneous rupture and destruction of OFP t-ELIP. The thrombolytic efficacy was significantly improved in the presence of rt-PA. These results suggest the combination of histotripsy and rt-PA could serve as a potent therapeutic strategy for the treatment of DVT.

  11. The active flexible wing aeroservoelastic wind-tunnel test program

    NASA Technical Reports Server (NTRS)

    Noll, Thomas; Perry, Boyd

    1989-01-01

    For a specific application of aeroservoelastic technology, Rockwell International Corporation developed a concept known as the Active Flexible Wing (AFW). The concept incorporates multiple active leading-and trailing-edge control surfaces with a very flexible wing such that wing shape is varied in an optimum manner resulting in improved performance and reduced weight. As a result of a cooperative program between the AFWAL's Flight Dynamics Laboratory, Rockwell, and NASA LaRC, a scaled aeroelastic wind-tunnel model of an advanced fighter was designed, fabricated, and tested in the NASA LaRC Transonic Dynamics Tunnel (TDT) to validate the AFW concept. Besides conducting the wind-tunnel tests NASA provided a design of an Active Roll Control (ARC) System that was implemented and evaluated during the tests. The ARC system used a concept referred to as Control Law Parameterization which involves maintaining constant performance, robustness, and stability while using different combinations of multiple control surface displacements. Since the ARC system used measured control surface stability derivatives during the design, the predicted performance and stability results correlated very well with test measurements.

  12. Influence of Friends on Children’s Physical Activity: A Review

    PubMed Central

    Maturo, Claire C.

    2013-01-01

    We examined evidence for friendship influences on children's physical activity (PA) through systematic searches of online databases in May 2012. We identified 106 studies (25 qualitative) published in English since 2000 that analyzed indicators of friendship influences (e.g., communication about PA, friends’ PA, and PA with friends) among persons younger than 19 years. Children's PA was positively associated with encouragement from friends (43 of 55 studies indicating a positive relationship), friends' own PA (30/35), and engagement with friends in PA (9/10). These findings are consistent with friends influencing PA, but most studies did not isolate influence from other factors that could explain similarity. Understanding friendship influences in childhood can facilitate the promotion of lifelong healthy habits. PA with friends should be considered in health promotion programs. PMID:23678914

  13. Synthesis of the SRM Fragmentation Activities Performed within VEGA Program

    NASA Astrophysics Data System (ADS)

    Jarry, A.; Meyer-Lasalle, F.; Le Falc'her, D.

    2013-09-01

    In the frame of VEGA program and especially the first flight on February 13, 2013, safety was a major concern. The default of the launcher and its impact on the close range were amongst the development phase topics because of the propellant masses at stake and the surrounding inhabited environment. A task group composed of members from ESA, CNES and industrial partners involved was formed for this matter.All SRMs are equipped with destruction chains. While P80 (first stage) is functioning, the Zefiri remain unpressurized. A scenario was set stating that the activation of Zefiri cutting chord creates an ignited gap inside the propellant. This ought to propagate till the inner bore, driving the explosion of the motor.This scenario was studied through small scale tests and numerical simulation, providing confidence on the feasibility of the destruction of the SRMs as well as inputs in terms of safety delays.

  14. 32 CFR 701.117 - Changes to PA systems of records.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... DEPARTMENT OF THE NAVY DOCUMENTS AFFECTING THE PUBLIC DON Privacy Program § 701.117 Changes to PA systems of... of all DON PA systems of records notices is available at http://www.privacy.navy.mil. (2) A DON... computer terminals at regional offices when the system was formerly used only at the headquarters would...

  15. 32 CFR 701.117 - Changes to PA systems of records.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... DEPARTMENT OF THE NAVY DOCUMENTS AFFECTING THE PUBLIC DON Privacy Program § 701.117 Changes to PA systems of... of all DON PA systems of records notices is available at http://www.privacy.navy.mil. (2) A DON... computer terminals at regional offices when the system was formerly used only at the headquarters would...

  16. 32 CFR 701.117 - Changes to PA systems of records.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 5 2013-07-01 2013-07-01 false Changes to PA systems of records. 701.117 Section 701.117 National Defense Department of Defense (Continued) DEPARTMENT OF THE NAVY UNITED STATES... DEPARTMENT OF THE NAVY DOCUMENTS AFFECTING THE PUBLIC DON Privacy Program § 701.117 Changes to PA systems...

  17. Pseudomonas aeruginosa PA1006 Is a Persulfide-Modified Protein That Is Critical for Molybdenum Homeostasis

    PubMed Central

    Tombline, Gregory; Schwingel, Johanna M.; Lapek, John D.; Friedman, Alan E.; Darrah, Thomas; Maguire, Michael; Van Alst, Nadine E.; Filiatrault, Melanie J.; Iglewski, Barbara H.

    2013-01-01

    A companion manuscript revealed that deletion of the Pseudomonas aeruginosa (Pae) PA1006 gene caused pleiotropic defects in metabolism including a loss of all nitrate reductase activities, biofilm maturation, and virulence. Herein, several complementary approaches indicate that PA1006 protein serves as a persulfide-modified protein that is critical for molybdenum homeostasis in Pae. Mutation of a highly conserved Cys22 to Ala or Ser resulted in a loss of PA1006 activity. Yeast-two-hybrid and a green-fluorescent protein fragment complementation assay (GFP-PFCA) in Pae itself revealed that PA1006 interacts with Pae PA3667/CsdA and PA3814/IscS Cys desulfurase enzymes. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) “top-down” analysis of PA1006 purified from Pae revealed that conserved Cys22 is post-translationally modified in vivo in the form a persulfide. Inductively-coupled-plasma (ICP)-MS analysis of ΔPA1006 mutant extracts revealed that the mutant cells contain significantly reduced levels of molybdenum compared to wild-type. GFP-PFCA also revealed that PA1006 interacts with several molybdenum cofactor (MoCo) biosynthesis proteins as well as nitrate reductase maturation factor NarJ and component NarH. These data indicate that a loss of PA1006 protein’s persulfide sulfur and a reduced availability of molybdenum contribute to the phenotype of a ΔPA1006 mutant. PMID:23409003

  18. Physical Activity Measures in the Healthy Communities Study

    PubMed Central

    Pate, Russell R.; McIver, Kerry; Colabianchi, Natalie; Troiano, Richard P.; Reis, Jared P.; Carroll, Dianna D.; Fulton, Janet E.

    2015-01-01

    The risk of obesity is reduced when youth engage in recommended levels of physical activity (PA). For that reason, public health organizations in the U.S. have encouraged communities to implement programs and policies designed to increase PA in youth, and many communities have taken on that challenge. However, the long-term effects of those programs and policies on obesity are largely unknown. The Healthy Communities Study (HCS) is a large-scale observational study of U.S. communities that is examining the characteristics of programs and policies designed to promote healthy behaviors (e.g., increase PA and improve diet) and determining their association with obesity-related outcomes. The purpose of this paper is to describe the methods used to measure PA in children and the personal and community factors that may influence it. The study used both self-reported and objective measures of PA, and measured personal, family, and home influences on PA via three constructs: (1) PA self-schema; (2) parental support; and (3) parental rules regarding PA. Neighborhood and community factors related to PA were assessed using three measures: (1) child perceptions of the neighborhood environment; (2) availability of PA equipment; and (3) attributes of the child's street segment via direct observation. School influences on children's PA were assessed via three constructs: (1) school PA policies; (2) child perceptions of the school PA environment; and (3) school outdoor PA environment. These measures will enable examination of the associations between characteristics of community PA programs and policies and obesity-related outcomes in children and youth. PMID:26384937

  19. Physical Activity Measures in the Healthy Communities Study.

    PubMed

    Pate, Russell R; McIver, Kerry L; Colabianchi, Natalie; Troiano, Richard P; Reis, Jared P; Carroll, Dianna D; Fulton, Janet E

    2015-10-01

    The risk of obesity is reduced when youth engage in recommended levels of physical activity (PA). For that reason, public health organizations in the U.S. have encouraged communities to implement programs and policies designed to increase PA in youth, and many communities have taken on that challenge. However, the long-term effects of those programs and policies on obesity are largely unknown. The Healthy Communities Study is a large-scale observational study of U.S. communities that is examining the characteristics of programs and policies designed to promote healthy behaviors (e.g., increase PA and improve diet) and determining their association with obesity-related outcomes. The purpose of this paper is to describe the methods used to measure PA in children and the personal and community factors that may influence it. The study used both self-reported and objective measures of PA, and measured personal, family, and home influences on PA via three constructs: (1) PA self-schema; (2) parental support; and (3) parental rules regarding PA. Neighborhood and community factors related to PA were assessed using three measures: (1) child perceptions of the neighborhood environment; (2) availability of PA equipment; and (3) attributes of the child's street segment via direct observation. School influences on children's PA were assessed via three constructs: (1) school PA policies; (2) child perceptions of the school PA environment; and (3) school outdoor PA environment. These measures will enable examination of the associations between characteristics of community PA programs and policies and obesity-related outcomes in children and youth.

  20. PA014-- Deception and Doubt --Strategies for Undermining and Supporting Global Climate Science--PA014

    NASA Astrophysics Data System (ADS)

    Nelson, C.

    2012-12-01

    DECEPTION AND DOUBT--STRATEGIES FOR UNDERMINING AND SUPPORTING GLOBAL CLIMATE SCIENCE--PA014 The fundamental strategy for undermining confidence in the now substantial scientific consensus about global warming is to sow doubt about the degree of consensus. Rather than mount an obvious anti-science stance, commercial interests seek to champion science, arguing for better science, more complete and definitive science. This strategy has a sixty-year history, beginning with the tobacco industry in the 1950s and proceeding through the chemical, energy, paint, and other industries. Thousands of faculty members have quietly sold themselves as public spokespersons or confidential consultants to industry in the service of this strategy. A multipart program--involving educating people about this history and exposing faculty collaboration--may help free climate science from those who aim to distort its conclusions.

  1. Increasing Physical Activity in Preschool: A Pilot Study to Evaluate Animal Trackers

    ERIC Educational Resources Information Center

    Williams, Christine L.; Carter, Betty Jean; Kibbe, Debra L.; Dennison, David

    2009-01-01

    Objective: This report describes a pilot study to evaluate Animal Trackers (AT), a preschool program designed to (1) increase structured physical activity (PA) during the preschool day; (2) increase practice of gross motor skills; (3) provide teachers with an easy-to-use PA program regardless of teacher experience; and (4) implement a teacher…

  2. 75 FR 69468 - Metlife Moosic, PA, Metlife Clarks Summit, PA; Notice of Affirmative Determination Regarding...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Employment and Training Administration Metlife Moosic, PA, Metlife Clarks Summit, PA; Notice of Affirmative Determination Regarding Application for Reconsideration By application dated August 2, 2010, the...

  3. An Emergent Language Program Framework: Actively Involving Learners in Needs Analysis.

    ERIC Educational Resources Information Center

    Savage, William; Storer, Graeme

    1992-01-01

    Relates the experience of the staff of an aquaculture outreach program in Northeast Thailand in implementing an English for special purposes program. By actively involving learners in both the needs analysis and program design, teachers were able to adapt the program content to the requirements of the students. (15 references) (JL)

  4. Creative Thinking Development Program for Learning Activity Management of Secondary School Teachers

    ERIC Educational Resources Information Center

    Pukdeewut, Sutinan; Chantarasombat, Chalard; Satapornwong, Pattananusorn

    2013-01-01

    The objectives of this research were: to design a creative thinking development program for learning activity management of secondary school teachers, and to study the program's efficiency and effectiveness of usage. The results of the study were as follows: the program includes the vision, principles, objectives, content, program development…

  5. Cultivating Leadership, Pedagogy and Programming for CSPAP and Healthy, Active Lifestyles at the University of Idaho

    ERIC Educational Resources Information Center

    Goc Karp, Grace; Brown, Helen; Scruggs, Philip W.; Berei, Catherine

    2017-01-01

    This article highlights processes for infusing comprehensive school physical activity programming (CSPAP) into the physical education teacher education (PETE) program at the University of Idaho (UI). The PETE program uses a modified leadership framework to target learning outcomes and activities pertinent to CSPAP. Student CSPAP knowledge and…

  6. Physical activity counseling in medical school education: a systematic review

    PubMed Central

    Dacey, Marie L.; Kennedy, Mary A.; Polak, Rani; Phillips, Edward M.

    2014-01-01

    Background Despite a large evidence base to demonstrate the health benefits of regular physical activity (PA), few physicians incorporate PA counseling into office visits. Inadequate medical training has been cited as a cause for this. This review describes curricular components and assesses the effectiveness of programs that have reported outcomes of PA counseling education in medical schools. Methods The authors systematically searched MEDLINE, EMBASE, PsychINFO, and ERIC databases for articles published in English from 2000 through 2012 that met PICOS inclusion criteria of medical school programs with PA counseling skill development and evaluation of outcomes. An initial search yielded 1944 citations, and 11 studies representing 10 unique programs met criteria for this review. These studies were described and analyzed for study quality. Strength of evidence for six measured outcomes shared by multiple studies was also evaluated, that is, students’ awareness of benefits of PA, change in students’ attitudes toward PA, change in personal PA behaviors, improvements in PA counseling knowledge and skills, self-efficacy to conduct PA counseling, and change in attitude toward PA counseling. Results Considerable heterogeneity of teaching methods, duration, and placement within the curriculum was noted. Weak research designs limited an optimal evaluation of effectiveness, that is, few provided pre-/post-intervention assessments, and/or included control comparisons, or met criteria for intervention transparency and control for risk of bias. The programs with the most evidence of improvement indicated positive changes in students’ attitudes toward PA, their PA counseling knowledge and skills, and their self-efficacy to conduct PA counseling. These programs were most likely to follow previous recommendations to include experiential learning, theoretically based frameworks, and students’ personal PA behaviors. Conclusions Current results provide some support for

  7. Activation of an adipogenic program in adult myoblasts with age.

    PubMed

    Taylor-Jones, Jane M; McGehee, Robert E; Rando, Thomas A; Lecka-Czernik, Beata; Lipschitz, David A; Peterson, Charlotte A

    2002-03-31

    Myoblasts isolated from mouse hindlimb skeletal muscle demonstrated increased adipogenic potential as a function of age. Whereas myoblasts from 8-month-old adult mice did not significantly accumulate terminal markers of adipogenesis regardless of culture conditions, myoblasts from 23-month-old mice accumulated fat and expressed genes characteristic of differentiated adipocytes, such as the fatty acid binding protein aP2. This change in differentiation potential was associated with a change in the abundance of the mRNA encoding the transcription factor C/EBPalpha, and in the relative abundance of PPARgamma2 to PPARgamma1 mRNAs. Furthermore, PPARgamma activity appeared to be regulated at the level of phosphorylation, being more highly phosphorylated in myoblasts isolated from younger animals. Although adipogenic gene expression in myoblasts from aged animals was activated, presumably in response to PPARgamma and C/EBPalpha, unexpectedly, myogenic gene expression was not effectively repressed. The Wnt signaling pathway may also alter differentiation potential in muscle with age. Wnt-10b mRNA was more abundantly expressed in muscle tissue and cultured myoblasts from adult compared with aged mice, resulting in stabilization of cytosolic beta-catenin, that may potentially contribute to inhibition of adipogenic gene expression in adult myoblasts. The changes reported here, together with those reported in bone marrow stroma with age, suggest that a default program may be activated in mesenchymal cells with increasing age resulting in a more adipogenic-like phenotype. Whether this change in differentiation potential contributes to the increased adiposity in muscle with age remains to be determined.

  8. Student Engagement in Research, Scholarship, and Creative Activity (SERSCA) Program: Sharing a Program Model from Design and Development through Evaluation

    ERIC Educational Resources Information Center

    Young, Shawna; Uy, Ana; Bell, Joyce

    2017-01-01

    The Student Engagement in Research, Scholarship, and Creative Activity (SERSCA) Program at California State University, Stanislaus provides support for student engagement in these areas from idea conception through dissemination. Through assistantships, mini-grants, the Student Research Competition, and travel grants, the Program is designed to…

  9. STEM after School: How to Design and Run Great Programs and Activities. A Guidebook for Program Leaders, Second Edition

    ERIC Educational Resources Information Center

    ExpandED Schools, 2014

    2014-01-01

    This guidebook was prepared by TASC (The After-School Corporation) and their Frontiers in Urban Science Education (FUSE) programs. FUSE is TASC's initiative to help more out-of-school-time programs and expanded learning time schools offer kids engaging, exciting and inspiring activities that promote science inquiry. The guidebook offers a a…

  10. National Dam Inspection Program. Robena Slurry Pond 6 (NDI Number PA 00197, Penn DER Number 30-26), Ohio River Basin, Tributary to Whiteley Creek, Greene County, Pennsylvania. Phase I Inspection Report

    DTIC Science & Technology

    1981-07-01

    not the dam constitutes a hazard to human life or property. 1.2 DESCRIPTION OF PROJECT a. General: Robena Slurry Pond 6 consists of an earth and...Surface Current Top of Dam 27.5 acres* Emergency Spillway Crest 24.7 acres g. Embankment Type Zoned Earth and Coarse Coal Refuse Length 1985 feet Height...CORPORATION RAW MATERIALS DIVISION FRICK DISTRICT PHASE : INSPECTION REPORT NATIONAL DAM INSPECTION PROGRAM liii JAN o 41982 -4 PREPARED FOR - "DPA

  11. National Dam Inspection Program. Laurel Hill Lake Dam (NDI I.D. Number PA-267, Penn.DER Number 56-66) Ohio River Basin. Laurel Hill Creek, Somerset County, Pennsylvania Phase I Inspection Report,

    DTIC Science & Technology

    1980-03-01

    AD-A GOG 1 73 ACKENH’EIL AND I ASSOCIATES INC BALTIMORE MO F /6 13/13NATIONAL DAM INSPECTION PROGRAM. LAUREL HILL LAKE DAM (NI I.D.--ETC(U) MAR...E2 APPENDIX F - REGIONAL GEOLOGY Regional Geology ...... ................ Fl Geologic Map.. ...... ................. F2 vi 4...Department of Environmental Resources, P. 0. Box 1467, Harrisburg, Pennsylvania 17120. F . PURPOSE OF DAM: The dam was constructed for use as a

  12. 7 CFR 272.5 - Program informational activities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... as specified at § 272.2(d)(2) to inform applicant and participant households about the importance of... program participants to participate in the Expanded Food and Nutrition Education Program (EFNEP) and... participant and applicant households of their Program rights and responsibilities. This information may...

  13. 7 CFR 272.5 - Program informational activities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... as specified at § 272.2(d)(2) to inform applicant and participant households about the importance of... program participants to participate in the Expanded Food and Nutrition Education Program (EFNEP) and... participant and applicant households of their Program rights and responsibilities. This information may...

  14. 7 CFR 272.5 - Program informational activities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... as specified at § 272.2(d)(2) to inform applicant and participant households about the importance of... program participants to participate in the Expanded Food and Nutrition Education Program (EFNEP) and... participant and applicant households of their Program rights and responsibilities. This information may...

  15. 7 CFR 272.5 - Program informational activities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... as specified at § 272.2(d)(2) to inform applicant and participant households about the importance of... program participants to participate in the Expanded Food and Nutrition Education Program (EFNEP) and... participant and applicant households of their Program rights and responsibilities. This information may...

  16. An investigation of commitment among participants in an extended day physical activity program.

    PubMed

    Schilling, T A

    2001-12-01

    This study examined underserved youth participants' perceptions of commitment to an extended day physical activity program using Hellison's (1995) responsibility model. Seven participants ranging in age from 12 to 15 years participated in the study. Two personal interviews and a focus group interview were used to obtain participants' perceptions ofprogram commitment. Results revealed that program organization, personal characteristics, development of interpersonal relationships, and the program environment influenced their program commitment. Participants described the nature of commitment in terms of program behavior, emotional involvement, and program history. While the type of activity was cited as a positive influence on program commitment, the specific activity could also serve as a barrier to program commitment. Implications for program development andfuture research are offered.

  17. 77 FR 50457 - Agency Information Collection Activities: Proposed Collection; Comment Request-WIC Program...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-21

    ... Supplemental Nutrition Program for Women, Infants and Children (WIC) Regulations (7 CFR part 246) for the... Special Supplemental Nutrition Program for Women, Infants and Children (WIC) is to provide supplemental... Food and Nutrition Service Agency Information Collection Activities: Proposed Collection;...

  18. Characteristics of Physical Activity Programs for Older Adults: Results of a Multisite Survey

    ERIC Educational Resources Information Center

    Hughes, Susan L.; Williams, Barbara; Molina, Lourdes C.; Bayles, Constance; Bryant, Lucinda L.; Harris, Jeffrey R.; Hunter, Rebecca; Ivey, Susan; Watkins, Ken

    2005-01-01

    Purpose: Although increased participation in physical activity by older adults is a major public health goal, little is known about the supply and use of physical activity programs in the United States. Design and Methods: Seven academic centers in diverse geographic areas surveyed physical activity programs for older adults. Five sites conducted…

  19. Exercise Level and Energy Expenditure in the Take 10![R] In-Class Physical Activity Program.

    ERIC Educational Resources Information Center

    Stewart, James A.; Dennison, David A.; Kohl, Harold W., III; Doyle, J. Andrew

    2004-01-01

    This study evaluated the effectiveness of an innovative, classroom-based physical activity prevention program designed to integrate academic curriculum elements along with a physical activity program in providing moderate-to-vigorous intensity physical activity. A convenience sample of three public school classrooms (one first, third, and fifth…

  20. 44 CFR 7.912 - To what programs or activities does this regulation apply?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... activities does this regulation apply? 7.912 Section 7.912 Emergency Management and Assistance FEDERAL... Financial Assistance From FEMA General § 7.912 To what programs or activities does this regulation apply? (a) The Act and this regulation apply to each FEMA recipient and to each program or activity operated...

  1. 25 CFR 170.152 - What transit facilities and activities are eligible for IRR Program funding?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 1 2013-04-01 2013-04-01 false What transit facilities and activities are eligible for... Eligibility Transit Facilities § 170.152 What transit facilities and activities are eligible for IRR Program funding? Transit facilities and activities eligible for IRR Program funding include, but are not...

  2. 25 CFR 170.152 - What transit facilities and activities are eligible for IRR Program funding?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 1 2011-04-01 2011-04-01 false What transit facilities and activities are eligible for... Eligibility Transit Facilities § 170.152 What transit facilities and activities are eligible for IRR Program funding? Transit facilities and activities eligible for IRR Program funding include, but are not...

  3. 25 CFR 170.152 - What transit facilities and activities are eligible for IRR Program funding?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 1 2012-04-01 2011-04-01 true What transit facilities and activities are eligible for... Eligibility Transit Facilities § 170.152 What transit facilities and activities are eligible for IRR Program funding? Transit facilities and activities eligible for IRR Program funding include, but are not...

  4. 25 CFR 170.152 - What transit facilities and activities are eligible for IRR Program funding?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 1 2014-04-01 2014-04-01 false What transit facilities and activities are eligible for... Eligibility Transit Facilities § 170.152 What transit facilities and activities are eligible for IRR Program funding? Transit facilities and activities eligible for IRR Program funding include, but are not...

  5. 25 CFR 170.152 - What transit facilities and activities are eligible for IRR Program funding?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false What transit facilities and activities are eligible for... Eligibility Transit Facilities § 170.152 What transit facilities and activities are eligible for IRR Program funding? Transit facilities and activities eligible for IRR Program funding include, but are not...

  6. 78 FR 45282 - Pennsylvania Disaster #PA-00058

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-26

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Pennsylvania Disaster PA-00058 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... determined to be adversely affected by the disaster: Primary Counties: Clearfield; Fayette;...

  7. Activity-induced regulation of myosin isoform distribution - Comparison of two contractile activity programs

    NASA Technical Reports Server (NTRS)

    Diffee, Gary M.; Caiozzo, Vince J.; Mccue, Samuel A.; Herrick, Robert E.; Baldwin, Kenneth M.

    1993-01-01

    This study examined the role of specific types of contractile activity in regulating myosin heavy chain (MHC) isoform expression in rodent soleus. A combination of hindlimb suspension (SN) and two programmed contractile training activity paradigms, either isometric contractile activity (ST-IM) or high-load slowly shortening isovelocity activity, were utilized. Both training paradigms increased muscle mass compared with SN alone. However, only ST-IM resulted in a partial prevention of the suspension-induced decrease in type I MHC. With the use of a fluorescently labeled antibody to type IIa MHC, the distribution of MHCs among fibers was examined immunohistochemically. In SN, the percentage of cells staining positive for type IIa MHC was increased but the staining intensity of the positively staining cells was unchanged compared with control cells. In the ST-IM soleus, the percentage of positively staining fibers was unchanged but the intensity of the positively staining cells was decreased compared with SN values. These results suggest that 1) isometric contractile activity is more effective than isovelocity activity in preventing suspension-induced shifts in soleus MHC distribution and 2) changes associated with both suspension and training occur in only a small number of fibers, with the majority of fibers apparently unresponsive to these interventions.

  8. UTSI/CFFF MHD Program Completion and Related Activity

    SciTech Connect

    1997-04-01

    Routine preventive maintenance of the DOE Coal Fired Flow Facility (CFFF) is being performed. Modernization programs, being funded under subcontract from Foster Wheeler Development by the DOE HIPPS Program, are being implemented on the coal processing system, the data acquisition and control system and control room. Environmental restoration actions continued with monitoring of groundwater wells and holding pond effluent. Actions are under way to dispose of spent seed/ash mixtures and excess coal remaining from the MHD POC program.

  9. Associations among social capital, parenting for active lifestyles, and youth physical activity in rural families living in upstate New York.

    PubMed

    Davison, Kirsten K; Nishi, Akihiro; Kranz, Sibylle; Wyckoff, Lynae; May, John J; Earle-Richardson, Giulia B; Strogatz, David S; Jenkins, Paul L

    2012-10-01

    While emerging research supports a positive relationship between social capital and youth physical activity (PA), few studies have examined possible mechanisms explaining this relationship and no studies have focused on rural youth. In this study, we examined parents' support of children's PA as an intermediary factor linking social capital and youth PA in a largely rural cross sectional sample of American children aged 6- to 19-years and their parents/guardians (N=767 families) living in upstate New York. Parents completed a self-administered survey assessing demographic factors, perceived social capital, support for children's PA, and children's PA including time spent outdoors and days per week of sufficient PA. Structural equation modeling was used to test the hypothesis that higher social capital is linked with higher parental support for PA and, in turn, higher PA in children. Analyses were conducted separately for younger (6-12 years) and older (13-19 years) children and controlled for demographic factors (child age, household education, participation in a food assistance program) and perceived neighborhood safety. Anticipated relationships among social capital, parents' activity-related support, and children's PA were identified for older, but not younger children. Findings suggest that parent support for children's PA is one possible mechanism linking social capital and youth PA and the parents of adolescents may rely more heavily on cues from their social environment to shape their approaches to supporting their children's PA than parents of younger children.

  10. National Dam Inspection Program. Montour Number 4 Refuse Bank (NDI number PA 00865, PENN DER Number 63-92), Ohio River Basin, Tributary to Chartiers Creek, Washington County, Pennsylvania. Phase I Inspection Report

    DTIC Science & Technology

    1981-07-01

    FLOOD HYDRORAPH PACKAGE (HEC-i) .- DAN SAFETY VERSION JULY 1978 I Al NATIIONAL PRlOGIAM FO TH :.INS•PEC’TION OF NON FIDER&•J DA 2 A2 H...TIME: 12.19.32 NATIONAL PROGRAM FOR TH INSPECTION OF NON FEDERAL DAMS HYDR1OLOGIC AND HYDRAULIC ANALYSIS OF MWlWOU #14 NW BANK PROBABLE •M FLOOD PMI...DATA m IUN TAREA SNAP TP.SA TRSPC RATIO INOWW S LOCAL 1 1 0.21 0.0 0.21 0.0 0.0 0 1 0 PRECIP DATA,. PFE PHS R6 R12 ]R24 R48 R72 R96 0.0 24.10 102.00

  11. 45 CFR 287.120 - What work activities may be provided under the NEW Program?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., alternative education, post secondary education, job readiness activity, job search, job skills training... employment, community service programs, entrepreneurial training, management training, job...

  12. The Feasibility of Using Nature-Based Settings for Physical Activity Programming: Views from Urban Youth and Program Providers

    ERIC Educational Resources Information Center

    Blanton, Jedediah E.; Oregon, Evelyn M.; Flett, M. Ryan; Gould, Daniel R.; Pfeiffer, Karin A.

    2013-01-01

    Background and Purpose: Given the urgency to design programs to increase physical activity, especially to combat obesity in children, the primary purpose of this study was to investigate perceptions and opinions of a nature-based physical activity intervention designed for low-income urban adolescents. Methods: Four focus groups of adolescents,…

  13. An Evaluation of Computer Based Activities in an Early Intervention Program. A Report to the Early Special Education Program.

    ERIC Educational Resources Information Center

    Elliott, Alison; Hall, Neil

    This study investigated ways of using computer-based learning activities to complement curriculum practices in preschool programs with an early intervention component through the use of computer-based learning activities. Particular attention was given to supporting the development of young children's early mathematical skills. The study took…

  14. Validity of a Questionnaire to Assess the Physical Activity Level in Coronary Artery Disease Patients

    ERIC Educational Resources Information Center

    Guiraud, Thibaut; Granger, Richard; Bousquet, Marc; Gremeaux, Vincent

    2012-01-01

    The aim of the study is to compare, in coronary artery disease patients, physical activity (PA) assessed with the Dijon Physical Activity Questionnaire (DPAQ) and the true PA objectively measured using an accelerometer. Seventy patients wore an accelerometer (MyWellness Key actimeter) throughout 1 week after a cardiac rehabilitation program that…

  15. Exploring Park Director Roles in Promoting Community Physical Activity

    PubMed Central

    Marsh, Terence; Derose, Kathryn Pitkin; Cohen, Deborah A.

    2013-01-01

    Background Parks provide numerous opportunities for physical activity (PA). Previous studies have evaluated parks’ physical features, but few have assessed how park staff influence PA. Methods We conducted semi -structured interviews with 49 park directors, focusing on perceptions of their role, park programs, marketing and outreach, external collaborations, and PA promotion. Directors also completed a questionnaire providing demographics, education and training, and other personal characteristics. Results Park directors’ descriptions of their roles varied widely, from primarily administrative to emphasizing community interaction, though most (70–80%) reported offering programs and community interaction as primary. Including PA in current programs and adding PA-specific programs were the most commonly reported ways of increasing PA. Also noted were facility and staffing improvements, and conducting citywide marketing. Many directors felt inadequately trained in marketing. Most parks reported community collaborations, but they appeared fairly superficial. An increasing administrative burden and bureaucracy were recurring themes throughout the interviews. Conclusions Staff training in marketing and operation of PA programs is needed. Partnerships with health departments and organizations can help facilitate the PA promotion potential of parks. As there are competing views of how parks should be managed, standardized benchmarks to evaluate efficiency may help to optimize usage and PA promotion. PMID:22733875

  16. Advanced Technological Education Program: 1995 Awards and Activities.

    ERIC Educational Resources Information Center

    National Science Foundation, Washington, DC. Directorate for Education and Human Resources.

    The Advanced Technological Education (ATE) program promotes exemplary improvement in advanced technological education at the national and regional level through support of curriculum development and program improvement at the undergraduate and secondary school levels, especially for technicians being educated for the high performance workplace of…

  17. An Argument for Including String Variables in Early Programming Activities.

    ERIC Educational Resources Information Center

    Winter, M. J.

    1982-01-01

    Suggests approach to teaching definitions and concepts of computer programs to learners at all age levels by using words (string manipulations) rather than numbers. An outline of a possible sequence for introducing the BASIC programming language is given. Two references are cited. (EJS)

  18. Biofuels Feedstock Development Program: 1995 activities and future directions

    SciTech Connect

    Ferrell, J.E.; Wright, L.L.; Tuskan, G.A.

    1995-12-31

    The Department of Energy`s (DOE`s) Biofuels Feedstock Development Program (BFDP) has led the nation in developing short-rotation woody crops (SRWC) and herbaceous energy crops (HEC) as feedstocks for renewable energy. Since 1978, approximately $60 million has been invested in research projects involving more than 100 federal, university, and private research institutions. The research has been highly leveraged with cost-sharing from USDA Forest Service, private industry, and state agencies. The performance of 154 woody species and 35 herbaceous species has been examined in field trials across the U.S. Results of this effort include the prescription of silvi-cultural systems for hybrid poplars and hybrid willows and agricultural systems for switchgrass. Selected clones of woody species are producing dry weight yields in research plots on agricultural land that are 3 to 7 times greater than those obtained from mixed species stands on forest land, and at least 2 times the yields of southern plantation pines. Selected switchgrass varieties are producing dry weight yields 2 to 7 times greater than average forage grass yields on pasture and crop land. Crop development research is continuing efforts to translate this potential to commercial enterprises over a more geographically diverse acreage. Environmental research on biomass crops is aimed at developing sustainable systems that will contribute to the biodiversity of agricultural landscapes. Systems integration and analysis aim to understand all factors affecting price and potential supplies of biomass crops at regional and national scales. Scale-up studies, feasibility analysis and demonstrations are establishing actual costs and facilitating the commercialization of integrated biomass systems. Information management and dissemination activities are facilitating the communication of results among a community of researchers, policy-makers, and potential users and producers of energy crops. 15 refs.

  19. Quality Assurance Program Plan for the radiological survey activities program --- Uranium Mill Tailings Remedial Action Project

    SciTech Connect

    Knott, R.R.; Little, C.A.

    1991-08-01

    The Pollutant Assessments Group (PAG) at the Grand Junction Office (GJO), Colorado, of Oak Ridge National Laboratory (ORNL) is responsible for surveying designated sites in the vicinity of 24 inactive mill sites involved in the Department of Energy's (DOE) Uranium Mill Tailings Remedial Action Project (UMTRAP). The purpose of these surveys is to provide a recommendation to DOE whether to include or exclude these sites from UMTRAP based on whether the on-site residual radioactive material (if any) originated from the former mill sites, and radiation levels on-site are in excess of appropriate Environmental Protection Agency (EPA) criteria. This report describes the Quality Assurance Plan (QAP) for the PAG in conducting all activities related to UMTRAP. All quality assurance provisions given by the DOE, DOE/UMTRA and ORNL organizations are integrated into this plan. Specifically, this report identifies the policies and procedures followed in accomplishing the PAG/UMTRA QA program, identifies those organizational units involved in the implementation of these procedures, and outlines the respective responsibilities of those groups. 11 refs., 6 figs., 3 tabs.

  20. Intravenous tPA Therapy Does Not Worsen Acute Intracerebral Hemorrhage in Mice

    PubMed Central

    Foerch, Christian; Rosidi, Nathanael L.; Schlunk, Frieder; Lauer, Arne; Cianchetti, Flor A.; Mandeville, Emiri; Arai, Ken; Yigitkanli, Kazim; Fan, Xiang; Wang, Xiaoying; van Leyen, Klaus; Steinmetz, Helmuth; Schaffer, Chris B.; Lo, Eng H.

    2013-01-01

    Tissue plasminogen activator (tPA) is the only FDA-approved treatment for reperfusing ischemic strokes. But widespread use of tPA is still limited by fears of inadvertently administering tPA in patients with intracerebral hemorrhage (ICH). Surprisingly, however, the assumption that tPA will worsen ICH has never been biologically tested. Here, we assessed the effects of tPA in two models of ICH. In a mouse model of collagenase-induced ICH, hemorrhage volumes and neurological deficits after 24 hrs were similar in saline controls and tPA-treated mice, whereas heparin-treated mice had 3-fold larger hematomas. In a model of laser-induced vessel rupture, tPA also did not worsen hemorrhage volumes, while heparin did. tPA is known to worsen neurovascular injury by amplifying matrix metalloproteinases during cerebral ischemia. In contrast, tPA did not upregulate matrix metalloproteinases in our mouse ICH models. In summary, our experimental data do not support the assumption that intravenous tPA has a deleterious effect in acute ICH. However, due to potential species differences and the inability of models to fully capture the dynamics of human ICH, caution is warranted when considering the implications of these findings for human therapy. PMID:23408937

  1. Intravenous tPA therapy does not worsen acute intracerebral hemorrhage in mice.

    PubMed

    Foerch, Christian; Rosidi, Nathanael L; Schlunk, Frieder; Lauer, Arne; Cianchetti, Flor A; Mandeville, Emiri; Arai, Ken; Yigitkanli, Kazim; Fan, Xiang; Wang, Xiaoying; van Leyen, Klaus; Steinmetz, Helmuth; Schaffer, Chris B; Lo, Eng H

    2013-01-01

    Tissue plasminogen activator (tPA) is the only FDA-approved treatment for reperfusing ischemic strokes. But widespread use of tPA is still limited by fears of inadvertently administering tPA in patients with intracerebral hemorrhage (ICH). Surprisingly, however, the assumption that tPA will worsen ICH has never been biologically tested. Here, we assessed the effects of tPA in two models of ICH. In a mouse model of collagenase-induced ICH, hemorrhage volumes and neurological deficits after 24 hrs were similar in saline controls and tPA-treated mice, whereas heparin-treated mice had 3-fold larger hematomas. In a model of laser-induced vessel rupture, tPA also did not worsen hemorrhage volumes, while heparin did. tPA is known to worsen neurovascular injury by amplifying matrix metalloproteinases during cerebral ischemia. In contrast, tPA did not upregulate matrix metalloproteinases in our mouse ICH models. In summary, our experimental data do not support the assumption that intravenous tPA has a deleterious effect in acute ICH. However, due to potential species differences and the inability of models to fully capture the dynamics of human ICH, caution is warranted when considering the implications of these findings for human therapy.

  2. Classroom Activities for the Interlakes Environmental and Outdoor Education Program.

    ERIC Educational Resources Information Center

    Chester Area Schools, SD. Interlakes Environmental and Outdoor Education Program.

    This teaching guide is a collection of environmental education activities written by various educators and environmentalists. The activities are designed for use in grades K-12, each activity being identified by grade level. The guide contains over 80 activities that are listed in a short introductory guide that precedes the actual activities. The…

  3. A snapshot of physical activity programs targeting Aboriginal and Torres Strait Islander people in Australia.

    PubMed

    Macniven, Rona; Elwell, Michelle; Ride, Kathy; Bauman, Adrian; Richards, Justin

    2017-01-19

    Issue addressed: Participation in physical activity programs can be an effective strategy to reduce chronic disease risk factors and improve broader social outcomes. Health and social outcomes are worse among Aboriginal and Torres Strait Islanders than non-Indigenous Australians, who represent an important group for culturally specific programs. The extent of current practice in physical activity programs is largely unknown. This study identifies such programs targeting this population group and describes their characteristics.Methods: Bibliographic and Internet searches and snowball sampling identified eligible programs operating between 2012 and 2015 in Australia (phase 1). Program coordinators were contacted to verify sourced information (phase 2). Descriptive characteristics were documented for each program.Results: A total of 110 programs were identified across urban, rural and remote locations within all states and territories. Only 11 programs were located through bibliographic sources; the remainder through Internet searches. The programs aimed to influence physical activity for health or broader social outcomes. Sixty five took place in community settings and most involved multiple sectors such as sport, health and education. Almost all were free for participants and involved Indigenous stakeholders. The majority received Government funding and had commenced within the last decade. More than 20 programs reached over 1000 people each; 14 reached 0-100 participants. Most included process or impact evaluation indicators, typically reflecting their aims.Conclusion: This snapshot provides a comprehensive description of current physical activity program provision for Aboriginal and Torres Strait Islander people across Australia. The majority of programs were only identified through the grey literature. Many programs collect evaluation data, yet this is underrepresented in academic literature.So what?: Capturing current practice can inform future efforts to

  4. The Federal Networking and Information Technology Research and Development Program: Funding Issues and Activities

    DTIC Science & Technology

    2004-07-01

    Information Technology Research and Development (NITRD) Program, to better reflect its expanded mission. The NITRD Program is composed of 12 agencies; its members work in collaboration to increase the overall effectiveness and productivity of federal information technology (IT) R&D. A National Coordinating Office coordinates the activities of the NITRD Program and reports to OSTP and the National Science and Technology

  5. Improvements to the FATOLA computer program including added actively controlled landing gear subroutines

    NASA Technical Reports Server (NTRS)

    Mall, G. H.

    1983-01-01

    Modifications to a multi-degree-of-freedom flexible aircraft take-off and landing analysis (FATOLA) computer program, including a provision for actively controlled landing gears to expand the programs simulation capabilities, are presented. Supplemental instructions for preparation of data and for use of the modified program are included.

  6. 231Pa and 233Pa Neutron-Induced Fission Data Analysis

    SciTech Connect

    Maslov, V.M.; Tetereva, N.A.; Baba, M.; Hasegawa, A.; Kornilov, N.V.; Kagalenko, A.B.

    2005-05-24

    The 231Pa and 233Pa neutron-induced fission cross-section database is analyzed within the Hauser-Feshbach approach. The consistency of neutron-induced fission cross-section data and data extracted from transfer reactions is investigated. The fission probabilities of Pa, fissioning in 231,233Pa(n,nf) reactions, are defined by fitting (3He,d) or (3He,t) transfer-reaction data. The present estimate of the 233Pa(n,f) fission cross section above the emissive fission threshold is supported by smooth level-density parameter systematics, validated in the case of the 231Pa(n,f) data description up to En =20 MeV.

  7. A Novel Antimicrobial Endolysin, LysPA26, against Pseudomonas aeruginosa

    PubMed Central

    Guo, Mingquan; Feng, Chunyan; Ren, Jie; Zhuang, Xuran; Zhang, Yan; Zhu, Yongzhang; Dong, Ke; He, Ping; Guo, Xiaokui; Qin, Jinhong

    2017-01-01

    The global increase in multidrug resistant (MDR) bacteria has led to phage therapy being refocused upon. A novel endolysin, LysPA26, containing a lysozyme-like domain, was screened against Pseudomonas aeruginosa in this study. It had activity against MDR P. aeruginosa without pretreatment with an outer-membrane permeabilizer. LysPA26 could kill up to 4 log units P. aeruginosa in 30 min. In addition, temperature and pH effect assays revealed that LysPA26 had good stability over a broad range of pH and temperatures. Moreover, LysPA26 could kill other Gram-negative bacteria, such as Klebsiella pneumonia, Acinetobacter baumannii and Escherichia coli, but not Gram-positive bacteria. Furthermore, LysPA26 could eliminate P. aeruginosa in biofilm formation. Our current results show that LysPA26 is a new and promising antimicrobial agent for the combat of Gram-negative pathogens. PMID:28289407

  8. Historically Black Colleges and Universities Nuclear Energy Training Program: Summary of program activities, fiscal year 1986

    SciTech Connect

    Not Available

    1987-04-27

    The Historically Black Colleges and Universities Nuclear Energy Training (HBCU NET) Program, funded by DOE, Office of Nuclear Energy and administered by ORAU, began in February 1984. The program provides support for training, study, research participation, and academic enrichment of students and faculty at designated HBCUs in nuclear science, nuclear engineering, and other nuclear-related technologes and disciplines. The program is composed of undergraduate scholarships, graduate fellowships, student and faculty research participation, and an annual student training institute.

  9. General Electric ATS Program technical review Phase 2 activities

    SciTech Connect

    Chance, T.; Smith, D.

    1995-12-31

    The Advanced Turbine Systems (ATS) Program Phase 2 objectives are to select a cycle, and to identify and resolve technical issues required to realize the ATS Program goals of 60% net combined cycle efficiency, single digit NOx, and a 10% electric power cost reduction, compared to current technology. The Phase 2 efforts have showns that the ATS Program goals are achievable. The GE Power Generation advanced gas turbine will use closed-loop steam cooling in the first two turbine stages and advanced coatings, seals and cooling designs to meet ATS performance and cost of electricity goals.

  10. The Adolescent Behavioral Activation Program: Adapting Behavioral Activation as a Treatment for Depression in Adolescence.

    PubMed

    McCauley, Elizabeth; Gudmundsen, Gretchen; Schloredt, Kelly; Martell, Christopher; Rhew, Isaac; Hubley, Samuel; Dimidjian, Sona

    2016-01-01

    This study aimed to examine implementation feasibility and initial treatment outcomes of a behavioral activation (BA) based treatment for adolescent depression, the Adolescent Behavioral Activation Program (A-BAP). A randomized, controlled trial was conducted with 60 clinically referred adolescents with a depressive disorder who were randomized to receive either 14 sessions of A-BAP or uncontrolled evidenced-based practice for depression. The urban sample was 64% female, predominantly Non-Hispanic White (67%), and had an average age of 14.9 years. Measures of depression, global functioning, activation, and avoidance were obtained through clinical interviews and/or through parent and adolescent self-report at preintervention and end of intervention. Intent-to-treat linear mixed effects modeling and logistic regression analysis revealed that both conditions produced statistically significant improvement from pretreatment to end of treatment in depression, global functioning, and activation and avoidance. There were no significant differences across treatment conditions. These findings provide the first step in establishing the efficacy of BA as a treatment for adolescent depression and support the need for ongoing research on BA as a way to enhance the strategies available for treatment of depression in this population.

  11. The Impact of a Community-Based Activity and Nutrition Program for Children.

    PubMed

    Valenzuela, Jessica M; Jacquez, Farrah; Pendery, Robin; Niemes, Lauren; Huddleston, David; Mitchell, Monica J

    2011-01-01

    There is consensus that community-wide integration of programs targeting pediatric obesity prevention is needed. The present article describes the growth, acceptability, and impact of a school- and community-based kids' marathon program targeting physical activity and nutrition. Youth in the program are encouraged to incrementally walk/run/jog a marathon and improve their diet through nutrition challenges. Participants track mileage and healthy eating over approximately 6 months, and complete a final marathon mile at the program's culminating event. Across 21 schools, 404 students between kindergarten and 8th grade reported on their experiences in the program. Also, 19 school coordinators rated their perceptions of the program's impact. While the data is preliminary and retrospective, it indicates that participants are highly engaged and that the program has potential to improve physical activity and other healthy lifestyle behaviors in many children. Strengths of the program, implications for obesity prevention, and future directions are also discussed.

  12. Ask Here PA: Large-Scale Synchronous Virtual Reference for Pennsylvania

    ERIC Educational Resources Information Center

    Mariner, Vince

    2008-01-01

    Ask Here PA is Pennsylvania's new statewide live chat reference and information service. This article discusses the key strategies utilized by Ask Here PA administrators to recruit participating libraries to contribute staff time to the service, the importance of centralized staff training, the main aspects of staff training, and activating the…

  13. New Insight into Metal Ion-Driven Catalysis of Nucleic Acids by Influenza PA-Nter

    PubMed Central

    Kotlarek, Daria; Worch, Remigiusz

    2016-01-01

    PA subunit of influenza RNA-dependent RNA polymerase deserves constantly increasing attention due to its essential role in influenza life cycle. N-terminal domain of PA (PA-Nter) harbors endonuclease activity, which is indispensable in viral transcription and replication. Interestingly, existing literature reports on in vitro ion preferences of the enzyme are contradictory. Some show PA-Nter activity exclusively with Mn2+, whereas others report Mg2+ as a natural cofactor. To clarify it, we performed a series of experiments with varied ion concentrations and substrate type. We observed cleavage in the presence of both ions, with a slight preference for manganese, however PA-Nter activity highly depended on the amount of residual, co-purified ions. Furthermore, to quantify cleavage reaction rate, we applied fluorescence cross-correlation spectroscopy (FCCS), providing highly sensitive and real-time monitoring of single molecules. Using nanomolar ssDNA in the regime of enzyme excess, we estimated the maximum reaction rate at 0.81± 0.38 and 1.38± 0.34 nM/min for Mg2+ and Mn2+, respectively. However, our calculations of PA-Nter ion occupancy, based on thermodynamic data, suggest Mg2+ to be a canonical metal in PA-Nter processing of RNA in vivo. Presented studies constitute a step toward better understanding of PA-Nter ion-dependent activity, which will possibly contribute to new successful inhibitor design in the future. PMID:27300442

  14. PA28 modulates antigen processing and viral replication during coxsackievirus B3 infection

    PubMed Central

    Respondek, Dorota; Voss, Martin; Kühlewindt, Ina; Klingel, Karin; Krüger, Elke

    2017-01-01

    The function of the proteasome is modulated at the level of subunit expression and by association with its regulatory complexes. During coxsackievirus B3 (CVB3) myocarditis, IFN-induced formation of immunoproteasomes (ip) is known to be critical for regulating immune modulating molecules. The function of the IFN-γ-inducible proteasome regulator subunits PA28 α and β, however, in this context was unknown. During viral myocarditis, we found an increased abundance of PA28β subunits in heart tissue. PA28α/β exists in PA28-20S-PA28 and PA700-20S-PA28 hybrid proteasome complexes in cells both with either predominant ip and standard proteasome (sp) expression. Being in line with reduced proteasome activity in PA28α/β-deficient cells, we observed increased levels of oxidized and poly-ubiquitinated proteins upon TLR3-activation in these cells. Moreover, PA28α/β is capable to interfere directly with viral replication of CVB3 and facilitates the generation of CVB3-derived MHC class I epitopes by the proteasome. In contrast to a distinct function of PA28α/β in vitro, gene ablation of PA28α/β in mice being on a genetic background with resistance towards the development of severe infection had no significant impact on disease progression. Other than reported for the ip, in this host PA28α/β is dispensable to meet the demand of increased peptide hydrolysis capacity by the proteasome during viral myocarditis. PMID:28278207

  15. PA28 modulates antigen processing and viral replication during coxsackievirus B3 infection.

    PubMed

    Respondek, Dorota; Voss, Martin; Kühlewindt, Ina; Klingel, Karin; Krüger, Elke; Beling, Antje

    2017-01-01

    The function of the proteasome is modulated at the level of subunit expression and by association with its regulatory complexes. During coxsackievirus B3 (CVB3) myocarditis, IFN-induced formation of immunoproteasomes (ip) is known to be critical for regulating immune modulating molecules. The function of the IFN-γ-inducible proteasome regulator subunits PA28 α and β, however, in this context was unknown. During viral myocarditis, we found an increased abundance of PA28β subunits in heart tissue. PA28α/β exists in PA28-20S-PA28 and PA700-20S-PA28 hybrid proteasome complexes in cells both with either predominant ip and standard proteasome (sp) expression. Being in line with reduced proteasome activity in PA28α/β-deficient cells, we observed increased levels of oxidized and poly-ubiquitinated proteins upon TLR3-activation in these cells. Moreover, PA28α/β is capable to interfere directly with viral replication of CVB3 and facilitates the generation of CVB3-derived MHC class I epitopes by the proteasome. In contrast to a distinct function of PA28α/β in vitro, gene ablation of PA28α/β in mice being on a genetic background with resistance towards the development of severe infection had no significant impact on disease progression. Other than reported for the ip, in this host PA28α/β is dispensable to meet the demand of increased peptide hydrolysis capacity by the proteasome during viral myocarditis.

  16. NCRP Vision for the Future and Program Area Committee Activities.

    PubMed

    Boice, John D

    2017-02-01

    The National Council on Radiation Protection and Measurements (NCRP) believes that the most critical need for the nation in radiation protection is to train, engage, and retain radiation professionals for the future. Not only is the pipeline shrinking, but for some areas there is no longer a pipe! When the call comes to respond, there may be no one to answer the phone! The NCRP "Where are the Radiation Professionals?" initiative, Council Committee (CC) 2, and this year's annual meeting are to focus our efforts to find solutions and not just reiterate the problems. Our next major initiative is CC 1, where the NCRP is making recommendations for the United States on all things dealing with radiation protection. Our last publication was NCRP Report No. 116, Limitation of Exposure to Ionizing Radiation, in 1993-time for an update. NCRP has seven active Program Area Committees on biology and epidemiology, operational concerns, emergency response and preparedness, medicine, environmental issues and waste management, dosimetry, and communications. A major scientific research initiative is the Million Person Study of Low Dose Radiation Health Effects. It includes workers from the Manhattan Project, nuclear weapons test participants (atomic veterans), industrial radiographers, and early medical workers such as radiologists and technologists. This research will answer the one major gap in radiation risk evaluation: what are the health effects when the exposure occurs gradually over time? Other cutting edge initiatives include a re-evaluation of science behind recommendations for lens of the eye dose limits, recommendations for emergency responders on dosimetry after a major radiological incident, guidance to the National Aeronautics and Space Administration with regard to possible central nervous system effects from galactic cosmic rays (the high energy, high mass particles bounding through space), re-evaluating the population exposure to medical radiation (NCRP Report No

  17. Pilot-Testing CATCH Early Childhood: A Preschool-Based Healthy Nutrition and Physical Activity Program

    ERIC Educational Resources Information Center

    Sharma, Shreela; Chuang, Ru-Jye; Hedberg, Ann Marie

    2011-01-01

    Background: The literature on theoretically-based programs targeting healthy nutrition and physical activity in preschools is scarce. Purpose: To pilot test CATCH Early Childhood (CEC), a preschool-based nutrition and physical activity program among children ages three to five in Head Start. Methods: The study was conducted in two Head Start…

  18. Case Study of an Institutionalized Urban Comprehensive School Physical Activity Program

    ERIC Educational Resources Information Center

    Doolittle, Sarah A.; Rukavina, Paul B.

    2014-01-01

    This single case study (Yin, 2009) compares an established urban physical education/ sport/physical activity program with two models: Comprehensive School Physical Activity Program/CSPAP (AAHPERD, 2013; CDC, 2013); and Lawson's propositions (2005) for sport, exercise and physical education for empowerment and community development to determine…

  19. Evaluating Active Parental Consent Procedures for School Programming: Addressing the Sensitive Topic of Suicide Prevention

    ERIC Educational Resources Information Center

    Totura, Christine M. Wienke; Kutash, Krista; Labouliere, Christa D.; Karver, Marc S.

    2017-01-01

    Background: Suicide is the second leading cause of death for adolescents. Whereas school-based prevention programs are effective, obtaining active consent for youth participation in public health programming concerning sensitive topics is challenging. We explored several active consent procedures for improving participation rates. Methods: Five…

  20. UWALK: the development of a multi-strategy, community-wide physical activity program.

    PubMed

    Jennings, Cally A; Berry, Tanya R; Carson, Valerie; Culos-Reed, S Nicole; Duncan, Mitch J; Loitz, Christina C; McCormack, Gavin R; McHugh, Tara-Leigh F; Spence, John C; Vallance, Jeff K; Mummery, W Kerry

    2016-06-09

    UWALK is a multi-strategy, multi-sector, theory-informed, community-wide approach using e and mHealth to promote physical activity in Alberta, Canada. The aim of UWALK is to promote physical activity, primarily via the accumulation of steps and flights of stairs, through a single over-arching brand. This paper describes the development of the UWALK program. A social ecological model and the social cognitive theory guided the development of key strategies, including the marketing and communication activities, establishing partnerships with key stakeholders, and e and mHealth programs. The program promotes the use of physical activity monitoring devices to self-monitor physical activity. This includes pedometers, electronic devices, and smartphone applications. In addition to entering physical activity data manually, the e and mHealth program provides the function for objective data to be automatically uploaded from select electronic devices (Fitbit®, Garmin and the smartphone application Moves) The RE-AIM framework is used to guide the evaluation of UWALK. Funding for the program commenced in February 2013. The UWALK brand was introduced on April 12, 2013 with the official launch, including the UWALK website on September 20, 2013. This paper describes the development and evaluation framework of a physical activity promotion program. This program has the potential for population level dissemination and uptake of an ecologically valid physical activity promotion program that is evidence-based and theoretically framed.

  1. Do Training Programs Work? An Assessment of Pharmacists Activities in the Field of Chemical Dependency.

    ERIC Educational Resources Information Center

    Brooks, Valerie G.; Brock, Tina Penick; Ahn, Jungeun

    2001-01-01

    Seeks to determine if pharmacists who attended a chemical dependency training program were performing more chemical dependency related activities. Results reveal that participants were more likely to perform the following activities: lecture to community groups about chemical dependency; participate in a pharmacists' recovery program; provide…

  2. 78 FR 46558 - Request for Information Regarding Nondiscrimination in Certain Health Programs or Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-01

    ..., sex, age, or disability in certain health programs and activities. Section 1557(c) of the Affordable... origin), Title IX of the Education Amendments of 1972 (Title IX), 20 U.S.C. 1681 et seq. (sex), the Age... prohibition of sex discrimination in Title IX applies only to education programs and activities of...

  3. 13 CFR 105.207 - Politically motivated activities with respect to the Minority Small Business Program.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Politically motivated activities with respect to the Minority Small Business Program. 105.207 Section 105.207 Business Credit and... activities with respect to the Minority Small Business Program. (a) Any employee who has authority to...

  4. 13 CFR 105.207 - Politically motivated activities with respect to the Minority Small Business Program.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Politically motivated activities with respect to the Minority Small Business Program. 105.207 Section 105.207 Business Credit and... activities with respect to the Minority Small Business Program. (a) Any employee who has authority to...

  5. 13 CFR 105.207 - Politically motivated activities with respect to the Minority Small Business Program.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Politically motivated activities with respect to the Minority Small Business Program. 105.207 Section 105.207 Business Credit and... activities with respect to the Minority Small Business Program. (a) Any employee who has authority to...

  6. 13 CFR 105.207 - Politically motivated activities with respect to the Minority Small Business Program.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Politically motivated activities with respect to the Minority Small Business Program. 105.207 Section 105.207 Business Credit and... activities with respect to the Minority Small Business Program. (a) Any employee who has authority to...

  7. 13 CFR 105.207 - Politically motivated activities with respect to the Minority Small Business Program.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Politically motivated activities with respect to the Minority Small Business Program. 105.207 Section 105.207 Business Credit and... activities with respect to the Minority Small Business Program. (a) Any employee who has authority to...

  8. 13 CFR 101.401 - What programs and activities of SBA are subject to this subpart?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What programs and activities of SBA are subject to this subpart? 101.401 Section 101.401 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION ADMINISTRATION Intergovernmental Partnership § 101.401 What programs and activities of SBA...

  9. 13 CFR 101.106 - Does Federal law apply to SBA programs and activities?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Does Federal law apply to SBA programs and activities? 101.106 Section 101.106 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION ADMINISTRATION Overview § 101.106 Does Federal law apply to SBA programs and activities? (a)...

  10. Evaluation of the Science Enrichment Activities (SEA) Program: A Decision Oriented Model.

    ERIC Educational Resources Information Center

    Linn, Marcia C.

    1978-01-01

    Three questions guided an evaluation of sixth and eighth grade science enrichment activities: (1) Does a free choice interactive program affect cognitive abilities? (2) Do students in a free choice program make predictable selections of activities based on their age, sex, or ability level? and (3) Are specific student choices associated with…

  11. 78 FR 18617 - Recreational Boating Safety Projects, Programs and Activities Funded Under Provisions of the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-27

    ... SECURITY Coast Guard Recreational Boating Safety Projects, Programs and Activities Funded Under Provisions of the Transportation Equity Act for the 21st Century; Accounting of ACTION: Notice. SUMMARY: In 1999... that a detailed accounting of the projects, programs, and activities funded under the...

  12. Exogenous t-PA Administration Increases Hippocampal Mature BDNF Levels. Plasmin- or NMDA-Dependent Mechanism?

    PubMed Central

    Rodier, Marion; Prigent-Tessier, Anne; Béjot, Yannick; Jacquin, Agnès; Mossiat, Claude; Marie, Christine; Garnier, Philippe

    2014-01-01

    Brain-derived neurotrophic factor (BDNF) through TrkB activation is central for brain functioning. Since the demonstration that plasmin is able to process pro-BDNF to mature BDNF and that these two forms have opposite effects on neuronal survival and plasticity, a particular attention has been paid to the link between tissue plasminogen activator (tPA)/plasmin system and BDNF metabolism. However, t-PA via its action on different N-methyl-D-aspartate (NMDA) receptor subunits is also considered as a neuromodulator of glutamatergic transmission. In this context, the aim of our study was to investigate the effect of recombinant (r)t-PA administration on brain BDNF metabolism in rats. In the hippocampus, we found that rt-PA (10 mg/kg) administration induced a progressive increase in mature BDNF levels associated with TrkB activation. In order to delineate the mechanistic involved, plasmin activity was assessed and its inhibition was attempted using tranexamic acid (30 or 300 mg/kg, i.v.) while NMDA receptors were antagonized with MK801 (0.3 or 3 mg/kg, i.p.) in combination with rt-PA treatment. Our results showed that despite a rise in rt-PA activity, rt-PA administration failed to increase hippocampal plasmin activity suggesting that the plasminogen/plasmin system is not involved whereas MK801 abrogated the augmentation in mature BDNF levels observed after rt-PA administration. All together, our results show that rt-PA administration induces increase in hippocampal mature BDNF expression and suggests that rt-PA contributes to the control of brain BDNF synthesis through a plasmin-independent potentiation of NMDA receptors signaling. PMID:24670989

  13. Exogenous t-PA administration increases hippocampal mature BDNF levels. plasmin- or NMDA-dependent mechanism?

    PubMed

    Rodier, Marion; Prigent-Tessier, Anne; Béjot, Yannick; Jacquin, Agnès; Mossiat, Claude; Marie, Christine; Garnier, Philippe

    2014-01-01

    Brain-derived neurotrophic factor (BDNF) through TrkB activation is central for brain functioning. Since the demonstration that plasmin is able to process pro-BDNF to mature BDNF and that these two forms have opposite effects on neuronal survival and plasticity, a particular attention has been paid to the link between tissue plasminogen activator (tPA)/plasmin system and BDNF metabolism. However, t-PA via its action on different N-methyl-D-aspartate (NMDA) receptor subunits is also considered as a neuromodulator of glutamatergic transmission. In this context, the aim of our study was to investigate the effect of recombinant (r)t-PA administration on brain BDNF metabolism in rats. In the hippocampus, we found that rt-PA (10 mg/kg) administration induced a progressive increase in mature BDNF levels associated with TrkB activation. In order to delineate the mechanistic involved, plasmin activity was assessed and its inhibition was attempted using tranexamic acid (30 or 300 mg/kg, i.v.) while NMDA receptors were antagonized with MK801 (0.3 or 3 mg/kg, i.p.) in combination with rt-PA treatment. Our results showed that despite a rise in rt-PA activity, rt-PA administration failed to increase hippocampal plasmin activity suggesting that the plasminogen/plasmin system is not involved whereas MK801 abrogated the augmentation in mature BDNF levels observed after rt-PA administration. All together, our results show that rt-PA administration induces increase in hippocampal mature BDNF expression and suggests that rt-PA contributes to the control of brain BDNF synthesis through a plasmin-independent potentiation of NMDA receptors signaling.

  14. Pre-Activity and Post-Activity Stretching Perceptions and Practices in NCAA Division I Volleyball Programs

    ERIC Educational Resources Information Center

    Judge, Lawrence W.; Bodey, Kimberly J.; Bellar, David; Bottone, Adam; Wanless, Elizabeth

    2010-01-01

    The purpose of this study was to determine if NCAA Division I women's volleyball programs were in compliance with suggested current pre- and post-activity stretching protocols. Questionnaires were sent to NCAA division I women's volleyball programs in the United States. Fifty six coaches (23 males & 33 females) participated in the study. Some…

  15. The Educational Value of High Risk Activities in the Physical Education Program: A Social Philosophical Perspective.

    ERIC Educational Resources Information Center

    Sparks, Robert E. C.

    A growing number of schools and institutions in North America have begun offering training in high risk activities such as high element rope courses, rock climbing, white water kayaking and canoeing, and scuba diving in conjunction with their regular physical education activity programs. High risk activities are those activities which occur in or…

  16. PA-30 Twin Comanche - NASA 808 in flight

    NASA Technical Reports Server (NTRS)

    1979-01-01

    Dryden Flight Research Center's Piper PA-30 Twin Commanche, which helped validate the RPRV concept, descends to a remotely controlled landing on Rogers Dry Lake, unassisted by the onboard pilot. A Piper PA-30 Twin Commanche, known as NASA 808, was used at the NASA Dryden Flight Research Center as a rugged workhorse in a variety of research projects associated with both general aviation and military projects. In the early 1970s, the PA-30, serial number 301498, was used to test a flight technique used to fly Remotely Piloted Research Vehicles (RPRV's). The technique was first tested with the cockpit windows of the light aircraft blacked out while the pilot flew the aircraft utilizing a television monitor which gave him a 'pilot's eye' view ahead of the aircraft. Later pilots flew the aircraft from a ground cockpit, a procedure used with all RPRV's. TV and two-way telemetry allow the pilot to be in constant control of the aircraft. The apparatus mounted over the cockpit is a special fish eye lens camera, used to obtain images that are transmitted to the ground based cockpit. This project paved the way for sophisticated, highly successful research programs involving high risk spin, stall, and flight control conditions, such as the HiMAT and the subscale F-15 remotely piloted vehicles. Over the years, NASA 808 has also been used for spin and stall research related to general aviation aircraft and also research to alleviate wake vortices behind large jetliners.

  17. Focus on Freshman: Basic Instruction Programs Enhancing Physical Activity

    ERIC Educational Resources Information Center

    Curry, Jarred; Jenkins, Jayne M.; Weatherford, Jennifer

    2015-01-01

    Physical activity sharply decreases after different life stages, particularly high school graduation to beginning university education. The purpose of this study was to investigate the effect of a specifically designed university physical activity class, Exercise Planning for Freshman (EPF), on students' physical activity and group cohesion…

  18. NASA/Aerospace Education Services Program. Classroom Activities.

    ERIC Educational Resources Information Center

    Nations, Jim, Comp.

    This document consists of a collection of classroom activities as they appeared in the "Aviation and Space Education News" from 1988 to 1991. The 45 activities in the document are organized in the following sections: (1) Aeronautics; (2) Earth Science; (3) Space Science; (4) Life in Space; (5) Rockets; and (6) Models. Each activity is…

  19. Health Promotion Efforts as Predictors of Physical Activity in Schools: An Application of the Diffusion of Innovations Model

    ERIC Educational Resources Information Center

    Glowacki, Elizabeth M.; Centeio, Erin E.; Van Dongen, Daniel J.; Carson, Russell L.; Castelli, Darla M.

    2016-01-01

    Background: Implementing a comprehensive school physical activity program (CSPAP) effectively addresses public health issues by providing opportunities for physical activity (PA). Grounded in the Diffusion of Innovations model, the purpose of this study was to identify how health promotion efforts facilitate opportunities for PA. Methods: Physical…

  20. Complementary interactions between command-like interneurons that function to activate and specify motor programs.

    PubMed

    Wu, Jin-Sheng; Wang, Nan; Siniscalchi, Michael J; Perkins, Matthew H; Zheng, Yu-Tong; Yu, Wei; Chen, Song-an; Jia, Ruo-nan; Gu, Jia-Wei; Qian, Yi-Qing; Ye, Yang; Vilim, Ferdinand S; Cropper, Elizabeth C; Weiss, Klaudiusz R; Jing, Jian

    2014-05-07

    Motor activity is often initiated by a population of command-like interneurons. Command-like interneurons that reliably drive programs have received the most attention, so little is known about how less reliable command-like interneurons may contribute to program generation. We study two electrically coupled interneurons, cerebral-buccal interneuron-2 (CBI-2) and CBI-11, which activate feeding motor programs in the mollusk Aplysia californica. Earlier work indicated that, in rested preparations, CBI-2, a powerful activator of programs, can trigger ingestive and egestive programs. CBI-2 reliably generated ingestive patterns only when it was repeatedly stimulated. The ability of CBI-2 to trigger motor activity has been attributed to the two program-promoting peptides it contains, FCAP and CP2. Here, we show that CBI-11 differs from CBI-2 in that it contains FCAP but not CP2. Furthermore, it is weak in its ability to drive programs. On its own, CBI-11 is therefore less effective as a program activator. When it is successful, however, CBI-11 is an effective specifier of motor activity; that is, it drives mostly ingestive programs. Importantly, we found that CBI-2 and CBI-11 complement each other's actions. First, prestimulation of CBI-2 enhanced the ability of CBI-11 to drive programs. This effect appears to be partly mediated by CP2. Second, coactivation of CBI-11 with CBI-2 makes CBI-2 programs immediately ingestive. This effect may be mediated by specific actions that CBI-11 exerts on pattern-generating interneurons. Therefore, different classes of command-like neurons in a motor network may make distinct, but potentially complementary, contributions as either activators or specifiers of motor activity.