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Sample records for activity soil respiration

  1. Soil CO2 constrain and distinction of root respiration and microbial activity by soil CO2 and CH4 profile

    NASA Astrophysics Data System (ADS)

    Ji, S.; Breecker, D.; Nie, J.

    2015-12-01

    Profiles of soil pore space CO2 and CH4 concentrations are rarely reported, especially from the same soils, yet are important for a number of applications. First, quantifying the component of respired CO2 in the soil pore spaces improves paleosol-based paleo-atmospheric CO2 estimates. Second, profiles can be used to estimate the average depth of biological activity (e.g. respiration and CH4 oxidation). Third, CH4 profiles, by identifying microbial activity, may help distinguish root/rhizosphere respiration from microbial decomposition. Here, we report soil CO2 and CH4 profiles measured at the Semi-Arid Climate Observatory and Laboratory (SACOL) on the Chinese Loess Plateau (CLP) at Lanzhou University, Gansu, China. Soil parent material on the site is mainly Quaternary aeolian loess and classifies as an Entisol. Soil respired CO2 (S(z) = soil CO2 - atmospheric CO2) is the most uncertain variable required to reconstruct ancient atmospheric CO2 concentrations from paleosol carbonates. Our direct soil pore space CO2 measurements show that S(z) values varied from ~100ppmV during the spring to ~2200ppmV during the summer. S(z) average 390 ± 30ppmV during May before the summer monsoon begins when soil temperature is increasing, soil water content is at a minimum and pedogenic carbonate may be forming. This value lies in the range of S(z) values previously estimated for surface Inceptisols (300 ± 100ppmV, Breecker 2013) and is lower than Pleistocene CLP paleosols (Da et al.,2015) in similar parent material. Our direct measurements of soil pore space CO2 thus support these previous independent S(z) estimates. We also investigate the average depth of CH4 oxidation and soil respiration, which range from 3-10cm and at least 20cm, respectively, using the shapes of soil gas profiles. Fitting observed soil CO2 and CH4 profiles with a production-diffusion model show that the average depth of CH4 oxidation was always at least 10 cm shallower than the average depth of respiration

  2. Soil Microbial Biomass, Basal Respiration and Enzyme Activity of Main Forest Types in the Qinling Mountains

    PubMed Central

    Cheng, Fei; Peng, Xiaobang; Zhao, Peng; Yuan, Jie; Zhong, Chonggao; Cheng, Yalong; Cui, Cui; Zhang, Shuoxin

    2013-01-01

    Different forest types exert essential impacts on soil physical-chemical characteristics by dominant tree species producing diverse litters and root exudates, thereby further regulating size and activity of soil microbial communities. However, the study accuracy is usually restricted by differences in climate, soil type and forest age. Our objective is to precisely quantify soil microbial biomass, basal respiration and enzyme activity of five natural secondary forest (NSF) types with the same stand age and soil type in a small climate region and to evaluate relationship between soil microbial and physical-chemical characters. We determined soil physical-chemical indices and used the chloroform fumigation-extraction method, alkali absorption method and titration or colorimetry to obtain the microbial data. Our results showed that soil physical-chemical characters remarkably differed among the NSFs. Microbial biomass carbon (Cmic) was the highest in wilson spruce soils, while microbial biomass nitrogen (Nmic) was the highest in sharptooth oak soils. Moreover, the highest basal respiration was found in the spruce soils, but mixed, Chinese pine and spruce stands exhibited a higher soil qCO2. The spruce soils had the highest Cmic/Nmic ratio, the greatest Nmic/TN and Cmic/Corg ratios were found in the oak soils. Additionally, the spruce soils had the maximum invertase activity and the minimum urease and catalase activities, but the maximum urease and catalase activities were found in the mixed stand. The Pearson correlation and principle component analyses revealed that the soils of spruce and oak stands obviously discriminated from other NSFs, whereas the others were similar. This suggested that the forest types affected soil microbial properties significantly due to differences in soil physical-chemical features. PMID:23840671

  3. Soil microbial biomass, basal respiration and enzyme activity of main forest types in the Qinling Mountains.

    PubMed

    Cheng, Fei; Peng, Xiaobang; Zhao, Peng; Yuan, Jie; Zhong, Chonggao; Cheng, Yalong; Cui, Cui; Zhang, Shuoxin

    2013-01-01

    Different forest types exert essential impacts on soil physical-chemical characteristics by dominant tree species producing diverse litters and root exudates, thereby further regulating size and activity of soil microbial communities. However, the study accuracy is usually restricted by differences in climate, soil type and forest age. Our objective is to precisely quantify soil microbial biomass, basal respiration and enzyme activity of five natural secondary forest (NSF) types with the same stand age and soil type in a small climate region and to evaluate relationship between soil microbial and physical-chemical characters. We determined soil physical-chemical indices and used the chloroform fumigation-extraction method, alkali absorption method and titration or colorimetry to obtain the microbial data. Our results showed that soil physical-chemical characters remarkably differed among the NSFs. Microbial biomass carbon (Cmic) was the highest in wilson spruce soils, while microbial biomass nitrogen (Nmic) was the highest in sharptooth oak soils. Moreover, the highest basal respiration was found in the spruce soils, but mixed, Chinese pine and spruce stands exhibited a higher soil qCO2. The spruce soils had the highest Cmic/Nmic ratio, the greatest Nmic/TN and Cmic/Corg ratios were found in the oak soils. Additionally, the spruce soils had the maximum invertase activity and the minimum urease and catalase activities, but the maximum urease and catalase activities were found in the mixed stand. The Pearson correlation and principle component analyses revealed that the soils of spruce and oak stands obviously discriminated from other NSFs, whereas the others were similar. This suggested that the forest types affected soil microbial properties significantly due to differences in soil physical-chemical features. PMID:23840671

  4. Changes in soil respiration after thinning activities in dense Aleppo pine forests

    NASA Astrophysics Data System (ADS)

    Llovet, Joan; Alonso, Macià; Cerdà, Artemi

    2015-04-01

    Forest fires are a widespread perturbation in Mediterranean areas, and they have tended to increase during the last decades (Pausas, 2004; Moreno et al, 1998). Aleppo pine (Pinus halepensis Mill) is dominant specie in some forest landscapes of western Mediterranean Basin, due to its capacity to colonize abandoned fields, and also due to afforestation practices mainly performed during the 20th century (Ruiz Navarro et al., 2009). Aleppo pine tends to die as consequence of forest fires, although it is able to disperse a high quantity of seeds which easily germinates. These dispersion and germination can result in dense forests with high inter and intra-specific competition, low diversity, low growth, and high fuel accumulation, increasing the risk of new forest fires. These forests of high density present ecological problems and management difficulties that require preventive treatments. Thinning treatments are common in these types of communities, but the management has to be oriented towards strengthening their functions. In the context of global change, better understandings of the implications of forest management practices in the carbon cycle are necessary. The objective of this study was to examine the evolution of seasonal soil respiration after treatment of selective thinning in dense Aleppo pine forests. The study area covers three localities placed in the Valencian Community (E Spain) affected by a forest fire in 1994. Thinning activities were done 16 years after the fire, reducing pine density from around 100,000 individuals per hectare to around 900 individuals per hectare. Soil respiration was measured in situ with a portable soil respiration instrument (LI-6400, LiCor, Lincoln, NB, USA) fitted with a soil respiration chamber (6400-09, LiCor, Lincoln, NB, USA). We installed 12 plots per treatment (control and thinned) and locality, being a total of 72 plots. We carried out 13 measurements covering a period of one year. We also estimated other related

  5. [Effects of Warming and Straw Application on Soil Respiration and Enzyme Activity in a Winter Wheat Cropland].

    PubMed

    Chen, Shu-tao; Sang, Lin; Zhang, Xu; Hu, Zheng-hua

    2016-02-15

    In order to investigate the effects of warming and straw application on soil respiration and enzyme activity, a field experiment was performed from November 2014 to May 2015. Four treatments, which were control (CK), warming, straw application, and warming and straw application, were arranged in field. Seasonal variability in soil respiration, soil temperature and soil moisture for different treatments were measured. Urease, invertase, and catalase activities for different treatments were measured at the elongation, booting, and anthesis stages. The results showed that soil respiration in different treatments had similar seasonal variation patterns. Seasonal mean soil respiration rates for the CK, warming, straw application, and warming and straw application treatments were 1.46, 1.96, 1.92, and 2.45 micromol x (m2 x s)(-1), respectively. ANOVA indicated that both warming and straw applications significantly (P < 0.05) enhanced soil respiration compared to the control treatment. The relationship between soil respiration and soil temperature in different treatments fitted with the exponential regression function. The exponential regression functions explained 34.3%, 28.1%, 24.6%, and 32.0% variations of soil respiration for CK, warming, straw application, and warming and straw application treatments, respectively. Warming and straw applications significantly (P < 0.05) enhanced urease, invertase, and catalase activities compared to CK. The relationship between soil respiration and urease activity fitted with a linear regression function, with the P value of 0.061. The relationship between soil respiration and invertase (P = 0.013), and between soil respiration and catalase activity (P = 0.002) fitted well with linear regression functions. PMID:27363163

  6. Predicting soil respiration from peatlands.

    PubMed

    Rowson, J G; Worrall, F; Evans, M G; Dixon, S D

    2013-01-01

    This study considers the relative performance of six different models to predict soil respiration from upland peat. Predicting soil respiration is important for global carbon budgets and gap filling measured data from eddy covariance and closed chamber measurements. Further to models previously published new models are presented using two sub-soil zones and season. Models are tested using data from the Bleaklow plateau, southern Pennines, UK. Presented literature models include ANOVA using logged environmental data, the Arrhenius equation, modified versions of the Arrhenius equation to include soil respiration activation energy and water table depth. New models are proposed including the introduction of two soil zones in the peat profile, and season. The first new model proposes a zone of high CO(2) productivity related to increased soil microbial CO(2) production due to the supply of labile carbon from plant root exudates and root respiration. The second zone is a deeper zone where CO(2) production is lower with less labile carbon. A final model allows the zone of high CO(2) production to become dormant during winter months when plants will senesce and will vary depending upon vegetation type within a fixed location. The final model accounted for, on average, 31.9% of variance in net ecosystem respiration within 11 different restoration sites whilst, using the same data set, the best fitting literature equation only accounted for 18.7% of the total variance. Our results demonstrate that soil respiration models can be improved by explicitly accounting for seasonality and the vertically stratified nature of soil processes. These improved models provide an enhanced basis for calculating the peatland carbon budgets which are essential in understanding the role of peatlands in the global C cycle. PMID:23178842

  7. Coupling aboveground and belowground activities using short term fluctuations in 13C composition of soil respiration

    NASA Astrophysics Data System (ADS)

    Epron, D.; Parent, F.; Grossiord, C.; Plain, C.; Longdoz, B.; Granier, A.

    2011-12-01

    There is a growing amount of evidence that belowground processes in forest ecosystems are tightly coupled to aboveground activities. Soil CO2 efflux, the largest flux of CO2 to the atmosphere, is dominated by root respiration and by respiration of microorganisms that find the carbohydrates required to fulfil their energetic costs in the rhizosphere. A close coupling between aboveground photosynthetic activity and soil CO2 efflux is therefore expected. The isotopic signature of photosynthates varies with time because photosynthetic carbon isotope discrimination is dynamically controlled by environmental factors. This temporal variation of δ13C of photosynthate is thought to be transferred along the tree-soil continuum and it will be retrieved in soil CO2 efflux after a time lag that reflects the velocity of carbon transport from canopy to belowground. However, isotopic signature of soil CO2 efflux is not solely affected by photosynthetic carbon discrimination, bur also by post photosynthetic fractionation, and especially by fractionation processes affecting CO2 during the transport from soil layers to surface. Tunable diode laser spectrometry is a useful tool to quantify short-term variation in δ13C of soil CO2 efflux and of CO2 in the soil atmosphere. We set up hydrophobic tubes to measure the vertical profile of soil CO2 concentration and its δ13C composition in a temperate beech forest, and we monitored simultaneously δ13C of trunk and soil CO2 efflux, δ13C of phloem exudate and δ13C of leaf sugars. We evidenced that temporal changes in δ13C of soil CO2 and soil CO2 efflux reflected changes in environmental conditions that affect photosynthetic discrimination and that soil CO2 was 4.4% enriched compared to soil CO2 efflux according to diffusion fractionation. However, this close coupling can be disrupted when advective transport of CO2 took place. We also reported evidences that temporal variations in the isotopic composition of soil CO2 efflux reflect

  8. Impact of different tillage treatments on soil respiration and microbial activity for different agricultural used soils in Austria

    NASA Astrophysics Data System (ADS)

    Klik, Andreas; Scholl, Gerlinde; Baatar, Undrakh-Od

    2015-04-01

    Soils can act as a net sink for sequestering carbon and thus attenuating the increase in atmospheric carbon dioxide if appropriate soil and crop management is applied. Adapted soil management strategies like less intensive or even no tillage treatments may result in slower mineralization of soil organic carbon and enhanced carbon sequestration. In order to assess the impact of different soil tillage systems on carbon dioxide emissions due to soil respiration and on soil biological activity parameters, a field study of three years duration (2007-2010)has been performed at different sites in Austria. Following tillage treatments were compared: 1) conventional tillage (CT) with plough with and without cover crop during winter period, 2) reduced tillage (RT) with cultivator with cover crop, and 3) no-till (NT) with cover crop. Each treatment was replicated three times. At two sites with similar climatic conditions but different soil textures soil CO2 efflux was measured during the growing seasons in intervals of one to two weeks using a portable soil respiration system consisting of a soil respiration chamber attached to an infrared gas analyzer. Additionally, concurrent soil temperature and soil water contents of the top layer (0-5 cm)were measured. For these and additional three other sites with different soil and climatic conditions soil samples were taken to assess the impact of tillage treatment on soil biological activity parameters. In spring, summer and autumn samples were taken from each plot at the soil depth of 0-10, 10-20, and 20-30 cm to analyze soil microbial respiration (MR), substrate induced respiration (SIR), beta-glucasidase activity (GLU) and dehydrogenase (BHY). Samples were sieved (2 mm) and stored at 4 °C in a refrigerator. Analyses of were performed within one month after sampling. The measurements show a high spatial variability of soil respiration data even within one plot. Nevertheless, the level of soil carbon dioxide efflux was similar for

  9. Modelling Soil respiration in agro-ecosystems

    NASA Astrophysics Data System (ADS)

    Delogu, Emilie; LeDantec, Valerie; Mordelet, Patrick; Buysse, Pauline; Aubinet, Marc; Pattey, Elizabeth

    2013-04-01

    A soil respiration model was developed to simulate soil respiration in crops on a daily time step. The soil heterotrophic respiration component was derived from Century (Parton et al., 1987). Soil organic carbon is divided into three major components including active, slow and passive soil carbon. Each pool has its own decomposition rate coefficient. Carbon flows between these pools are controlled by carbon inputs (crop residues), decomposition rate and microbial respiration loss parameters, both of which are a function of soil texture, soil temperature and soil water content. The model assumes that all C decompositions flows are associated with microbial activity and that microbial respiration occurs for each of these flows. Heterotrophic soil respiration is the sum of all these microbial respiration processes. To model the soil autotrophic respiration component, maintenance respiration is calculated from the nitrogen content and assuming an exponential relationship to account for temperature dependence (Ryan et al., 1991). Growth respiration is calculated assuming a dependence on both growth rate and construction cost of the considered organ (MacCree et al., 1982) A database, made of four different soil and climate conditions in mid-latitude was used to study the two components of the soil respiration model in wheat fields. Soil respiration were measured in three winter wheat fields at Lamasquère (43°49'N, 01°23'E, 2007) and Auradé (43°54'N, 01°10'E, 2008), South-West France and Lonzée (50°33'N, 4°44'E, 2007), Belgium, and in a spring wheat field at Ottawa (45°22'N, 75°43'W, 2007, 2011), Ontario, Canada. Manual closed chambers were used in the French sites. The Belgium and Canadian sites were equipped with automated closed chamber systems, which continuously collected 30-min soil respiration exchanges. All the sites were also equipped with eddy flux towers. When eddy flux data were collected over bare soil, the net ecosystem exchange (NEE) was equal to

  10. Influence of soil moisture on soil respiration

    NASA Astrophysics Data System (ADS)

    Fer, Miroslav; Kodesova, Radka; Nikodem, Antonin; Klement, Ales; Jelenova, Klara

    2015-04-01

    The aim of this work was to describe an impact of soil moisture on soil respiration. Study was performed on soil samples from morphologically diverse study site in loess region of Southern Moravia, Czech Republic. The original soil type is Haplic Chernozem, which was due to erosion changed into Regosol (steep parts) and Colluvial soil (base slope and the tributary valley). Soil samples were collected from topsoils at 5 points of the selected elevation transect and also from the parent material (loess). Grab soil samples, undisturbed soil samples (small - 100 cm3, and large - 713 cm3) and undisturbed soil blocks were taken. Basic soil properties were determined on grab soil samples. Small undisturbed soil samples were used to determine the soil water retention curves and the hydraulic conductivity functions using the multiple outflow tests in Tempe cells and a numerical inversion with HYDRUS 1-D. During experiments performed in greenhouse dry large undisturbed soil samples were wetted from below using a kaolin tank and cumulative water inflow due to capillary rise was measured. Simultaneously net CO2 exchange rate and net H2O exchange rate were measured using LCi-SD portable photosynthesis system with Soil Respiration Chamber. Numerical inversion of the measured cumulative capillary rise data using the HYDRUS-1D program was applied to modify selected soil hydraulic parameters for particular conditions and to simulate actual soil water distribution within each soil column in selected times. Undisturbed soil blocks were used to prepare thin soil sections to study soil-pore structure. Results for all soil samples showed that at the beginning of soil samples wetting the CO2 emission increased because of improving condition for microbes' activity. The maximum values were reached for soil column average soil water content between 0.10 and 0.15 cm3/cm3. Next CO2 emission decreased since the pore system starts filling by water (i.e. aggravated conditions for microbes

  11. BOREAS TE-5 Soil Respiration Data

    NASA Technical Reports Server (NTRS)

    Hall, Forrest G. (Editor); Curd, Shelaine (Editor); Ehleriinger, Jim; Brooks, J. Renee; Flanagan, Larry

    2000-01-01

    The BOREAS TE-5 team collected measurements in the NSA and SSA on gas exchange, gas composition, and tree growth. Soil respiration data were collected from 26-May-94 to 07-Sep-94 in the BOREAS NSA and SSA to compare the soil respiration rates in different forest sites using a LI-COR 6200 soil respiration chamber (model 6299). The data are stored in tabular ASCII files. The data files are available on a CD-ROM (see document number 20010000884), or from the Oak Ridge National Laboratory (ORNL) Distrobuted Activity Archive Center (DAAC).

  12. Agriculture intensification decreases soil C content and respiration activity in a Mediterranean Vertisol

    NASA Astrophysics Data System (ADS)

    Farina, Roberta; Francaviglia, Rosa; Felici, Barbara; Renzi, Gianluca; Troccoli, Antonio

    2016-04-01

    Adoption of intensive and non-conservative farming practices in Mediterranean areas, often causes a strong reduction of soil organic C, with major side effects on soil functioning and CO2 emissions to atmosphere. The purpose of our research was to evaluate the effect of durum wheat-(Triticum durum Desf.) (DW) based rotations, common in Southern Italy, on soil organic C content and soil potential respiration, after 19 years of cultivation. The rotation experiment was carried out since 1992 in Foggia (Apulia, Italy) at the experimental farm of the Cereal Research Centre in a clayey vertisol. Here we report results concerning two rotations, among seven: continuous durum wheat (CDW) and bare fallow-durum wheat-durum wheat- (BF-DW-DW) compared with an adjoining soil, covered with permanent grassland undisturbed, since 1972, considered at steady state. Results showed a negative trend of soil C in both rotations. The C reduction respect to the undisturbed soil (14.5 g C kg-1 of soil) were 0.15 and 0.13% for CDW and BF-DW-DW, respectively. Daily soil potential respiration was always higher in the undisturbed soil: it was 13.65, 10.46 and 8.64 mg C-CO2/kg soil day-1, for undisturbed soil, BF-DW-DW and DWC respectively. The cumulative respiration in 28 days for CDW and BF-DW-DW rotations compared with undisturbed soil was lower by 23 and 32%, respectively. Among the two rotations compared, BF-DW-DW showed to be slightly more conservative than the DWC rotation for soil C, even though none of the two rotations was able to keep the soil C level at values comparable to steady state, due both to soil disturbance and to lower C inputs respect to the permanent cover.

  13. Seasonal Variation in Soil Microbial Biomass, Bacterial Community Composition and Extracellular Enzyme Activity in Relation to Soil Respiration in a Northern Great Plains Grassland

    NASA Astrophysics Data System (ADS)

    Wilton, E.; Flanagan, L. B.

    2014-12-01

    Soil respiration rate is affected by seasonal changes in temperature and moisture, but is this a direct effect on soil metabolism or an indirect effect caused by changes in microbial biomass, bacterial community composition and substrate availability? In order to address this question, we compared continuous measurements of soil and plant CO2 exchange made with an automatic chamber system to analyses conducted on replicate soil samples collected on four dates during June-August. Microbial biomass was estimated from substrate-induced respiration rate, bacterial community composition was determined by 16S rRNA amplicon pyrosequencing, and β-1,4-N-acetylglucosaminidase (NAGase) and phenol oxidase enzyme activities were assayed fluorometrically or by absorbance measurements, respectively. Soil microbial biomass declined from June to August in strong correlation with a progressive decline in soil moisture during this time period. Soil bacterial species richness and alpha diversity showed no significant seasonal change. However, bacterial community composition showed a progressive shift over time as measured by Bray-Curtis dissimilarity. In particular, the change in community composition was associated with increasing relative abundance in the alpha and delta classes, and declining abundance of the beta and gamma classes of the Proteobacteria phylum during June-August. NAGase showed a progressive seasonal decline in potential activity that was correlated with microbial biomass and seasonal changes in soil moisture. In contrast, phenol oxidase showed highest potential activity in mid-July near the time of peak soil respiration and ecosystem photosynthesis, which may represent a time of high input of carbon exudates into the soil from plant roots. This input of exudates may stimulate the activity of phenol oxidase, a lignolytic enzyme involved in the breakdown of soil organic matter. These analyses indicated that seasonal change in soil respiration is a complex

  14. Soil Respiration in Response to Landscape Position

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Variations in soil type, due to landscape position, may influence soil respiration. This study was conducted to determine how landscape position (summit, side-slope, and depression) influences heterotrophic and autotrophic soil respiration. Soil respiration was determined at three landscape positio...

  15. Tillage Effects on Soil Properties & Respiration

    NASA Astrophysics Data System (ADS)

    Rusu, Teodor; Bogdan, Ileana; Moraru, Paula; Pop, Adrian; Duda, Bogdan; Cacovean, Horea; Coste, Camelia

    2015-04-01

    Soil tillage systems can be able to influence soil compaction, water dynamics, soil temperature and soil structural condition. These processes can be expressed as changes of soil microbiological activity, soil respiration and sustainability of agriculture. Objectives of this study were: 1) to assess the effects of tillage systems (Conventional System-CS, Minimum Tillage-MT, No-Tillage-NT) on soil compaction, soil temperature, soil moisture and soil respiration and 2) to establish the relationship that exists in changing soil properties. Three treatments were installed: CS-plough + disc; MT-paraplow + rotary grape; NT-direct sowing. The study was conducted on an Argic-Stagnic Faeoziom. The MT and NT applications reduce or completely eliminate the soil mobilization, due to this, soil is compacted in the first year of application. The degree of compaction is directly related to soil type and its state of degradation. The state of soil compaction diminished over time, tending toward a specific type of soil density. Soil moisture was higher in NT and MT at the time of sowing and in the early stages of vegetation and differences diminished over time. Moisture determinations showed statistically significant differences. The MT and NT applications reduced the thermal amplitude in the first 15 cm of soil depth and increased the soil temperature by 0.5-2.20C. The determinations confirm the effect of soil tillage system on soil respiration; the daily average was lower at NT (315-1914 mmoli m-2s-1) and followed by MT (318-2395 mmoli m-2s-1) and is higher in the CS (321-2480 mmol m-2s-1). Comparing with CS, all the two conservation tillage measures decreased soil respiration, with the best effects of no-tillage. An exceeding amount of CO2 produced in the soil and released into the atmosphere, resulting from aerobic processes of mineralization of organic matter (excessive loosening) is considered to be not only a way of increasing the CO2 in the atmosphere, but also a loss of

  16. Soil Respiration and Student Inquiry: A Perfect Match

    ERIC Educational Resources Information Center

    Hoyt, Catherine Marie; Wallenstein, Matthew David

    2011-01-01

    This activity explores the cycling of carbon between the atmosphere (primarily as CO[subscript 2]) and biomass in plants, animals, and microscopic organisms. Students design soil respiration experiments using a protocol that resembles current practice in soil ecology. Three methods for measuring soil respiration are presented. Student-derived…

  17. Short-Term Responses of Soil Respiration and C-Cycle Enzyme Activities to Additions of Biochar and Urea in a Calcareous Soil.

    PubMed

    Song, Dali; Xi, Xiangyin; Huang, Shaomin; Liang, Guoqing; Sun, Jingwen; Zhou, Wei; Wang, Xiubin

    2016-01-01

    Biochar (BC) addition to soil is a proposed strategy to enhance soil fertility and crop productivity. However, there is limited knowledge regarding responses of soil respiration and C-cycle enzyme activities to BC and nitrogen (N) additions in a calcareous soil. A 56-day incubation experiment was conducted to investigate the combined effects of BC addition rates (0, 0.5, 1.0, 2.5 and 5.0% by mass) and urea (U) application on soil nutrients, soil respiration and C-cycle enzyme activities in a calcareous soil in the North China Plain. Our results showed soil pH values in both U-only and U plus BC treatments significantly decreased within the first 14 days and then stabilized, and CO2emission rate in all U plus BC soils decreased exponentially, while there was no significant difference in the contents of soil total organic carbon (TOC), dissolved organic carbon (DOC), total nitrogen (TN), and C/N ratio in each treatment over time. At each incubation time, soil pH, electrical conductivity (EC), TOC, TN, C/N ratio, DOC and cumulative CO2 emission significantly increased with increasing BC addition rate, while soil potential activities of the four hydrolytic enzymes increased first and then decreased with increasing BC addition rate, with the largest values in the U + 1.0%BC treatment. However, phenol oxidase activity in all U plus BC soils showed a decreasing trend with the increase of BC addition rate. Our results suggest that U plus BC application at a rate of 1% promotes increases in hydrolytic enzymes, does not highly increase C/N and C mineralization, and can improve in soil fertility. PMID:27589265

  18. Biomass and respiration activity of soil microorganisms in anthropogenically transformed ecosystems (Moscow region)

    NASA Astrophysics Data System (ADS)

    Ivashchenko, K. V.; Ananyeva, N. D.; Vasenev, V. I.; Kudeyarov, V. N.; Valentini, R.

    2014-09-01

    In the forest, meadow, arable, and urban ecosystems (recreational, residential, and industrial zones) of Sergiev Posad, Shatura, Serpukhov, and Serebryanye Prudy districts of Moscow region, spatially separated sites (3-5 points per site) have been randomly selected and soil samples have been taken from the 0-10 (plant litter excluded) and 10- to 150-cm layers (a total of 201 samples have been taken). In the samples, the microbial biomass carbon (Cmic), the rate of the basal (microbial) respiration (BR), and the physical parameters (the particle size distribution (PSD), organic carbon (Corg), pH, heavy metals, and nutrients (NPK)) have been determined. High spatial variability has been revealed for Cmic and BR in all the ecosystems and the functional zones of the studied districts, and a clear tendency of a decrease in these parameters has been shown in the arable soils (by 1.4-3.2 times) and the industrial zone (by 1.7-3.3 times) compared to the natural analogues and other corresponding functional zones. It has been shown that the spatial distribution of the microbiological parameters is significantly ( p ≤ 0.05) affected by the physicochemical properties of the soil (Cmic by the PSD and PSD × Corg; BR by the pH and pH × NPK; contributions of 40 and 63%, respectively), as well as by the type of ecosystem and the region of study (the contribution of the sum of these factors to the Cmic and BR was 56 and 67%, respectively). A tendency toward the deterioration of the functioning of the microbial community under the anthropogenic transformation of the soil has been shown. The contribution of the urban soils as a potential source of CO2 emission to the atmosphere has been calculated and discussed.

  19. Dependence of soil respiration on soil temperature and soil moisture in successional forests in Southern China

    USGS Publications Warehouse

    Tang, X.-L.; Zhou, G.-Y.; Liu, S.-G.; Zhang, D.-Q.; Liu, S.-Z.; Li, J.; Zhou, C.-Y.

    2006-01-01

    The spatial and temporal variations in soil respiration and its relationship with biophysical factors in forests near the Tropic of Cancer remain highly uncertain. To contribute towards an improvement of actual estimates, soil respiration rates, soil temperature, and soil moisture were measured in three successional subtropical forests at the Dinghushan Nature Reserve (DNR) in southern China from March 2003 to February 2005. The overall objective of the present study was to analyze the temporal variations of soil respiration and its biophysical dependence in these forests. The relationships between biophysical factors and soil respiration rates were compared in successional forests to test the hypothesis that these forests responded similarly to biophysical factors. The seasonality of soil respiration coincided with the seasonal climate pattern, with high respiration rates in the hot humid season (April-September) and with low rates in the cool dry season (October-March). Soil respiration measured at these forests showed a clear increasing trend with the progressive succession. Annual mean (?? SD) soil respiration rate in the DNR forests was (9.0 ?? 4.6) Mg CO2-C/hm2per year, ranging from (6.1 ?? 3.2) Mg CO2-C/hm2per year in early successional forests to (10.7 ?? 4.9) Mg CO2-C/hm2 per year in advanced successional forests. Soil respiration was correlated with both soil temperature and moisture. The T/M model, where the two biophysical variables are driving factors, accounted for 74%-82% of soil respiration variation in DNR forests. Temperature sensitivity decreased along progressive succession stages, suggesting that advanced-successional forests have a good ability to adjust to temperature. In contrast, moisture increased with progressive succession processes. This increase is caused, in part, by abundant respirators in advanced-successional forest, where more soil moisture is needed to maintain their activities. ?? 2006 Institute of Botany, Chinese Academy of

  20. Autotrophic and heterotrophic components of soil respiration in permafrost zone.

    NASA Astrophysics Data System (ADS)

    Udovenko, Maria; Goncharova, Olga

    2016-04-01

    Soil carbon dioxide emissions production is an important integral indicator of soil biological activity and it includes several components: the root respiration and microbial decomposition of organic matter. Separate determination of the components of soil respiration is necessary for studying the balance of carbon in the soil and to assessment its potential as a sink or source of carbon dioxide. The aim of this study was testing field methods of separate determination of root and microbial respiration in soils of north of West Siberia. The research took place near the town Nadym, Yamalo-Nenets Autonomous District (north of West Siberia).The study area was located in the northern taiga with sporadic permafrost. Investigations were carried out at two sites: in forest and in frozen peatland. 3 methods were tested for the separation of microbial and root respiration. 1) "Shading"; 2) "Clipping"(removing the above-ground green plant parts); 3)a modified method of roots exclusion (It is to compare the emission of soils of "peat spots", devoid of vegetation and roots, and soils located in close proximity to the spots on which there is herbaceous vegetation and moss). For the experiments on methods of "Shading" and "Clipping" in the forest and on the frozen peatland ware established 12 plots, 1 x 1 m (3 plots in the forest and at 9 plots on frozen peatland; 4 of them - control).The criterions for choosing location sites were the similarity of meso- and microrelief, the same depth of permafrost, the same vegetation. Measurement of carbon dioxide emissions (chamber method) was carried out once a day, in the evening, for a week. Separation the root and microbial respiration by "Shading" showed that in the forest the root respiration contribution is 5%, and microbial - 95%. On peatlands root respiration is 41%, 59% of the microbial. In the experiment "Clipping" in peatlands root respiration is 56%, the microbial respiration - 44%, in forest- root respiration is 17%, and

  1. Separation of root respiration from total soil respiration using carbon-13 labelling during free-air carbon dioxide enrichment (FACE)

    SciTech Connect

    Andrews, J.A.; Harrison, K.G.; Matamala, R.; Schlesinger, W.H.

    1999-10-01

    Soil respiration constitutes a major component of the global carbon cycle and is likely to be altered by climate change. However, there is an incomplete understanding of the extent to which various processes contribute to total soil respiration, especially the contributions of root and rhizosphere respiration. Here, using a stable carbon isotope tracer, the authors separate the relative contributions of root and soil heterotrophic respiration to total soil respiration in situ. The Free-Air Carbon dioxide Enrichment (FACE) facility in the Duke University Forest (NC) fumigates plots of an undisturbed loblolly pine (Pinus taeda L.) forest with CO{sub 2} that is strongly depleted in {sup 13}C. This labeled CO{sub 2} is found in the soil pore space through live root and mycorrhizal respiration and soil heterotroph respiration of labile root exudates. By measuring the depletion of {sup 13}CO{sub 2} in the soil system, the authors found that the rhizosphere contribution to soil CO{sub 2} reflected the distribution of fine roots in the soil and that late in the growing season roots contributed 55% of total soil respiration at the surface. This estimate may represent an upper limit on the contribution of roots to soil respiration because high atmospheric CO{sub 2} often increases in root density and/or root activity in the soil.

  2. Forest thinning and soil respiration in a ponderosa pine plantation in the Sierra Nevada.

    PubMed

    Tang, Jianwu; Qi, Ye; Xu, Ming; Misson, Laurent; Goldstein, Allen H

    2005-01-01

    Soil respiration is controlled by soil temperature, soil water, fine roots, microbial activity, and soil physical and chemical properties. Forest thinning changes soil temperature, soil water content, and root density and activity, and thus changes soil respiration. We measured soil respiration monthly and soil temperature and volumetric soil water continuously in a young ponderosa pine (Pinus ponderosa Dougl. ex P. Laws. & C. Laws.) plantation in the Sierra Nevada Mountains in California from June 1998 to May 2000 (before a thinning that removed 30% of the biomass), and from May to December 2001 (after thinning). Thinning increased the spatial homogeneity of soil temperature and respiration. We conducted a multivariate analysis with two independent variables of soil temperature and water and a categorical variable representing the thinning event to simulate soil respiration and assess the effect of thinning. Thinning did not change the sensitivity of soil respiration to temperature or to water, but decreased total soil respiration by 13% at a given temperature and water content. This decrease in soil respiration was likely associated with the decrease in root density after thinning. With a model driven by continuous soil temperature and water time series, we estimated that total soil respiration was 948, 949 and 831 g C m(-2) year(-1) in the years 1999, 2000 and 2001, respectively. Although thinning reduced soil respiration at a given temperature and water content, because of natural climate variability and the thinning effect on soil temperature and water, actual cumulative soil respiration showed no clear trend following thinning. We conclude that the effect of forest thinning on soil respiration is the combined result of a decrease in root respiration, an increase in soil organic matter, and changes in soil temperature and water due to both thinning and interannual climate variability. PMID:15519986

  3. Forest Soil Respiration: Identifying Sources and Controls

    NASA Astrophysics Data System (ADS)

    Högberg, P.

    2008-12-01

    Most of the respiration in forests comes from the soil. This flux is composed of two components, autotrophic and heterotrophic respiration. In a strict sense the former should be plant belowground respiration only, but the term is used here to denote respiration by roots, their mycorrhizal fungal symbionts and other closely associated organisms dependent on recent photosynthate. Heterotrophs are organisms using organic matter, chiefly above- and belowground litters, as substrate (i.e. substrates of in general much higher ecosystem age). Because of the complexity of the plant-soil system, the component fluxes are difficult to study. I will discuss results of different approaches to partition soil respiratory components and to study their controls. The focus will be on northern boreal forests. In these generally strongly nitrogen-limited forests, the autotrophic respiration equals or exceeds the heterotrophic component. The large autotrophic component reflects high plant allocation of C to roots and mycorrhizal fungi in response to the low N supply. A physiological manipulation, girdling, which stops the flow of photosynthates to roots, showed that autotrophic respiration could account for as much as 70% in N-limited forests, but only 40% in fertilized forests. Also using girdling, we could show that a shift to lower summertime temperature leads to a decrease in heterotrophic but not in autotrophic activity, suggesting substrate (photosynthate) limitation of the latter. Physiological manipulations like girdling and trenching cannot be used to reveal the finer details of soil C dynamics. Natural abundance stable isotope (13C) and 14C approaches also have their limitations if a high resolution in terms of time, space and organism is required. A very high resolution can, of course, be obtained in studies of laboratory micro- or mesocosms, but the possibility to extend the interpretation of their results to the field may be questioned. In the CANIFLEX (CArbon NItrogen

  4. CORRELATIONS BETWEEN PESTICIDE TRANSFORMATION RATE AND MICROBIAL RESPIRATION ACTIVITY IN SOIL OF DIFFERENT ECOSYSTEMS

    EPA Science Inventory

    Cecil sandy loam soils (ultisol) from forest (coniferous and deciduous), pasture, and arable ecosystems were sampled (0-10 cm) in the vicinity of Athens, GA, USA. Soil from each site was subdivided into three portions, consisting of untreated soil (control) as well as live and s...

  5. Can the gradient method improve our ability to predict soil respiration?

    NASA Astrophysics Data System (ADS)

    Phillips, Claire; Nickerson, Nicholas; Risk, Dave

    2015-04-01

    Soil surface flux measurements integrate respiration across steep vertical gradients of soil texture, moisture, temperature, and carbon substrates. Although there are benefits to integrating complex soil processes in a single surface measure, i.e. for constructing soil carbon budgets, one serious drawback of studying only surface respiration is the difficulty in generating predictive relationships from environmental drivers. For example, the relationship between depth-integrated soil respiration and temperature measured at a single discreet depth (apparent temperature sensitivity) can bear little resemblance to the temperature sensitivity of soil respiration within soil layers (actual temperature sensitivity). Here we present several examples of how the inferred environmental sensitivity of soil respiration can be improved from observations of CO2 flux profiles in contrast to surface fluxes alone. We present a theoretical approach for estimating the temperature sensitivity of soil respiration in situ, called the weighted heat flux approach, which avoids much of the hysteresis produced by typical respiration-temperature comparisons. The weighted heat flux approach gives more accurate estimates of within-soil temperature sensitivity, and is arguably the most theoretically robust analytical temperature model available. We also show how soil drying influences the effectiveness of the weighted heat flux approach, as well as the relative activity of discreet soil layers and specific soil organisms, such as mycorrhizal fungi. The additional information provided by within-soil flux profiles can improve the fidelity of both probabilistic and mechanistic soil respiration models

  6. Partitioning Soil Respiration Between Autotrophic and Heterotrophic Components in a Mature Boreal Black Spruce Stand

    NASA Astrophysics Data System (ADS)

    Gaumont-Guay, D.; Black, T. A.; Barr, A. G.; Jassal, R. S.; Morgenstern, K.; Nesic, Z.

    2005-12-01

    A root-exclusion experiment conducted in mature boreal black spruce stand (125 year-old) in Saskatchewan, Canada, from September 2003 to December 2004 allowed the partitioning of soil respiration between autotrophic (roots, mycorrhizae and decomposers associated with the rhizosphere) and heterotrophic (free-living organisms) components using continuous automated chamber measurements of soil CO2 efflux. The exclusion of live roots caused a 25% reduction in soil respiration three weeks after the application of the treatment in September 2003, which suggested a strong link between tree photosynthesis and belowground respiration processes. Annual estimates of autotrophic and heterotrophic respiration were 324 and 230 g C m-2 y-1 in 2004, accounting for 53 and 38% of soil respiration, respectively, after correcting for the decomposition of roots killed by trenching (78 g C m-2 y-1). The remainder (57 g C m-2 y-1) originated from live-moss respiration. Over the course of the year, there was a gradual transition from heterotrophic to autotrophic-dominated respiration with three distinctive phases: (1) autotrophic respiration was negligible during winter when the trees were dormant; (2) heterotrophic respiration dominated soil respiration during the shoulder periods of April-May and October-November when soil temperature was low; (3) autotrophic respiration exceeded heterotrophic respiration from mid-July to mid-September when soil temperature was high and trees were active. Both components of respiration increased exponentially with soil temperature during the growing season but autotrophic respiration showed greater temperature sensitivity than heterotrophic respiration. The replenishment of soil water following spring snowmelt induced a sustained increase in heterotrophic respiration. Pulses in autotrophic respiration were observed during summer following large rainfalls that were attributed to rhizosphere priming effects. After normalizing autotrophic respiration for

  7. Invariant soil water potential at zero microbial respiration explained by hydrological discontinuity in dry soils

    NASA Astrophysics Data System (ADS)

    Manzoni, S.; Katul, G.

    2014-10-01

    Soil microbial respiration rates decrease with soil drying, ceasing below water potentials around -15 MPa. A proposed mechanism for this pattern is that under dry conditions, microbes are substrate limited because solute diffusivity is halted due to breaking of water film continuity. However, pore connectivity estimated from hydraulic conductivity and solute diffusivity (at Darcy's scale) is typically interrupted at much less negative water potentials than microbial respiration (-0.1 to -1 MPa). It is hypothesized here that the more negative respiration thresholds than at the Darcy's scale emerge because microbial activity is restricted to microscale soil patches that retain some hydrological connectivity even when it is lost at the macroscale. This hypothesis is explored using results from percolation theory and meta-analyses of respiration-water potential curves and hydrological percolation points. When reducing the spatial scale from macroscale to microscale, hydrological and respiration thresholds become consistent, supporting the proposed hypothesis.

  8. How Ecosystems Breathe: Measuring Respiration of Soil

    NASA Astrophysics Data System (ADS)

    McTammany, M. E.

    2005-05-01

    Curriculum for general ecology labs often uses in-lab exercises and computer simulations to demonstrate ecological principles rather than experimental field projects. In addition, ecosystem processes can be difficult to incorporate into general ecology labs because the techniques require sophisticated equipment or complex field designs. As an alternative to in-lab projects, I have integrated field measurement of soil respiration into my general ecology lab to teach students aspects of experimental design (sampling, replication, error, etc.) and to demonstrate how organism-level processes operate beyond single organisms in nature and are influenced by environmental conditions. In a program laden with biomedical interests, analogies between organisms and ecosystems are quite appealing to students. Students in my general ecology course complete a 2-week field project in which they measure soil respiration inside a dark microcosm chamber. We use 10% KOH to trap evolved CO2 and titrate unreacted KOH in lab using 1N HCl. The protocol is simple, only requires some chemicals, and can be used in many different habitats (including flower beds on campus) quite easily. Potential experiments could involve varying environmental conditions, such as soil moisture, nutrient availability, gaseous environment, carbon supply, or temperature, to affect soil respiration rate.

  9. [Effects of Tillage on Soil Respiration and Root Respiration Under Rain-Fed Summer Corn Field].

    PubMed

    Lu, Xing-li; Liao, Yun-cheng

    2015-06-01

    To explore the effects of different tillage systems on soil respiration and root respiration under rain-fed condition. Based on a short-term experiment, this paper investigated soil respiration in summer corn growth season under four tillage treatments including subsoiling tillage (ST), no tillage (NT), rotary tillage (RT) and moldboard plow tillage (CT). The contribution of root respiration using root exclusion method was also discussed. The results showed that soil respiration rate presented a single peak trend under four tillage methods during the summer corn growing season, and the maximum value was recorded at the heading stage. The trends of soil respiration were as follows: heading stage > flowering stage > grain filling stage > maturity stage > jointing stage > seedling stage. The trends of soil respiration under different tillage systems were as follows: CT > ST > RT > NT. There was a significant correlation between soil respiration rate and soil temperatures (P < 0.05), which could explain 35%-75% variability of soil respiration using exponential function equation. However, there was no significant correlation between soil respiration rate and soil moisture. Root respiration accounted for 45.13%-56.86% of the proportion of soil respiratio n with the mean value 51.72% during the summer corn growing season under different tillage systems. Therefore, root exclusion method could be used to study the contribution of crop growth to carbon emission, to compare effects of different tillage systems on the contribution of root respiration provides the bases for selecting the measures to slow down the decomposition of soil carbon. PMID:26387335

  10. Soil respiration, labile carbon pools, and enzyme activities as affected by tillage practices in a tropical rice-maize-cowpea cropping system.

    PubMed

    Neogi, S; Bhattacharyya, P; Roy, K S; Panda, B B; Nayak, A K; Rao, K S; Manna, M C

    2014-07-01

    In order to identify the viable option of tillage practices in rice-maize-cowpea cropping system that could cut down soil carbon dioxide (CO2) emission, sustain grain yield, and maintain better soil quality in tropical low land rice ecology soil respiration in terms of CO2 emission, labile carbon (C) pools, water-stable aggregate C fractions, and enzymatic activities were investigated in a sandy clay loam soil. Soil respiration is the major pathway of gaseous C efflux from terrestrial systems and acts as an important index of ecosystem functioning. The CO2-C emissions were quantified in between plants and rows throughout the year in rice-maize-cowpea cropping sequence both under conventional tillage (CT) and minimum tillage (MT) practices along with soil moisture and temperature. The CO2-C emissions, as a whole, were 24 % higher in between plants than in rows, and were in the range of 23.4-78.1, 37.1-128.1, and 28.6-101.2 mg m(-2) h(-1) under CT and 10.7-60.3, 17.3-99.1, and 17.2-79.1 mg m(-2) h(-1) under MT in rice, maize, and cowpea, respectively. The CO2-C emission was found highest under maize (44 %) followed by rice (33 %) and cowpea (23 %) irrespective of CT and MT practices. In CT system, the CO2-C emission increased significantly by 37.1 % with respect to MT on cumulative annual basis including fallow. The CO2-C emission per unit yield was at par in rice and cowpea signifying the beneficial effect of MT in maintaining soil quality and reduction of CO2 emission. The microbial biomass C (MBC), readily mineralizable C (RMC), water-soluble C (WSC), and permanganate-oxidizable C (PMOC) were 19.4, 20.4, 39.5, and 15.1 % higher under MT than CT. The C contents in soil aggregate fraction were significantly higher in MT than CT. Soil enzymatic activities like, dehydrogenase, fluorescein diacetate, and β-glucosidase were significantly higher by 13.8, 15.4, and 27.4 % under MT compared to CT. The soil labile C pools, enzymatic activities, and

  11. Soil Respiration under Different Land Uses in Eastern China

    PubMed Central

    Fan, Li-Chao; Yang, Ming-Zhen; Han, Wen-Yan

    2015-01-01

    Land-use change has a crucial influence on soil respiration, which further affects soil nutrient availability and carbon stock. We monitored soil respiration rates under different land-use types (tea gardens with three production levels, adjacent woodland, and a vegetable field) in Eastern China at weekly intervals over a year using the dynamic closed chamber method. The relationship between soil respiration and environmental factors was also evaluated. The soil respiration rate exhibited a remarkable single peak that was highest in July/August and lowest in January. The annual cumulative respiration flux increased by 25.6% and 20.9% in the tea garden with high production (HP) and the vegetable field (VF), respectively, relative to woodland (WL). However, no significant differences were observed between tea gardens with medium production (MP), low production (LP), WL, and VF. Soil respiration rates were significantly and positively correlated with organic carbon, total nitrogen, and available phosphorous content. Each site displayed a significant exponential relationship between soil respiration and soil temperature measured at 5 cm depth, which explained 84–98% of the variation in soil respiration. The model with a combination of soil temperature and moisture was better at predicting the temporal variation of soil respiration rate than the single temperature model for all sites. Q10 was 2.40, 2.00, and 1.86–1.98 for VF, WL, and tea gardens, respectively, indicating that converting WL to VF increased and converting to tea gardens decreased the sensitivity of soil respiration to temperature. The equation of the multiple linear regression showed that identical factors, including soil organic carbon (SOC), soil water content (SWC), pH, and water soluble aluminum (WSAl), drove the changes in soil respiration and Q10 after conversion of land use. Temporal variations of soil respiration were mainly controlled by soil temperature, whereas spatial variations were

  12. Investigation of soil carbon sequestration processes in a temperate deciduous forest using soil respiration experiments

    NASA Astrophysics Data System (ADS)

    Schütze, Claudia; Marañón-Jiménez, Sara; Zöphel, Hendrik; Gimper, Sebastian; Dienstbach, Laura; Garcia Quirós, Inmaculada; Cuntz, Matthias; Rebmann, Corinna

    2016-04-01

    Considering the carbon cycles of terrestrial ecosystems, soils represent a major long-term carbon storage pool. However, the storage capacity depends on several impact parameters based on biotic factors (e.g. vegetation activity, microbial activity, nutrient availability, interactions between vegetation and microbial activity) and abiotic driving factors (e.g. soil moisture, soil temperature, soil composition). Especially, increases in vegetation and microbial activity can lead to raised soil carbon release detectable as higher soil respiration rates. Within the frame of the ICOS project, several soil respiration experiments are under consideration at the temperate deciduous forest site "Hohes Holz" (Central Germany). These experiments started in May 2014. Soil respiration data acquisition was carried out using 8 automatic continuous chambers (LI-COR) and 60 different plots for bi-weekly survey chamber measurements in order to clarify the controlling factors for soil CO2 emissions such as litter availability, above- and belowground vegetation, and activation of microbial activity with temperature, soil moisture and root occurrence. Hence, several treatments (trenched, non-trenched, litter supply) were investigated on different plots within the research area. The data analysis of the 20-month observation period reveals preliminary results of the study. Obviously, significant differences between the trenched and the non-trenched plots concerning the CO2 emissions occurred. Increased soil carbon releases are supposed to be associated to the activation of microbial mineralization of soil organic matter by root inputs. Furthermore, depending on the amount of litter supply, different levels of activation were observed. The data of the continuous chamber measurements with a temporal resolution of one hour sampling interval can be used to show the dependence on above described biogeochemical processes due to abiotic controlling factors. Especially, soil moisture as a

  13. Soil respiration partition and its components in the total agro-ecosystem respiration

    NASA Astrophysics Data System (ADS)

    Delogu, Emilie; LeDantec, Valerie; Mordelet, Patrick; Buysse, Pauline; Aubinet, Marc; Pattey, Elizabeth; Mary, Bruno

    2013-04-01

    Close to 15% of the Earth's terrestrial surface is used for cropland. In the context of global warming, and acknowledged by the Kyoto Protocol, agricultural soils could be a significant sink for atmospheric CO2. Understanding the factors influencing carbon fluxes of agricultural soils is essential for implementing efficient mitigation practices. Most of the soil respiration modeling studies was carried out in forest ecosystems, but only a few was carried out in agricultural ecosystems. In the study, we evaluated simple formalisms to model soil respiration using wheat data from four contrasting geographical mi-latitude regions. Soil respiration were measured in three winter wheat fields at Lamasquère (43°49'N, 01°23'E, 2007) and Auradé (43°54'N, 01°10'E, 2008), South-West France and Lonzée (50°33'N, 4°44'E, 2007), Belgium, and in a spring wheat field at Ottawa (45°22'N, 75°43'W, 2007, 2011), Ontario, Canada. Manual closed chambers were used in the French sites. The Belgium and Canadian sites were equipped with automated closed chamber systems, which continuously collected 30-min soil respiration exchanges. All the sites were also equipped with eddy flux towers. When eddy flux data were collected over bare soil, the net ecosystem exchange (NEE) was equal to soil respiration exchange. These NEE data were used to validate the model. Different biotic and abiotic descriptors were used to model daily soil respiration and its heterotrophic and autotrophic components: soil temperature, soil relative humidity, Gross Primary Productivity (GPP), shoot biomass, crop height, with different formalisms. It was interesting to conclude that using biotic descriptors did not improve the performances of the model. In fact, a combination of abiotic descriptors (soil humidity and soil temperature) allowed significant model formalism to model soil respiration. The simple soil respiration model was used to calculate the heterotrophic and autotrophic source contributions to

  14. Soil and sediment bacteria capable of aerobic nitrate respiration.

    PubMed Central

    Carter, J P; Hsaio, Y H; Spiro, S; Richardson, D J

    1995-01-01

    Several laboratory strains of gram-negative bacteria are known to be able to respire nitrate in the presence of oxygen, although the physiological advantage gained from this process is not entirely clear. The contribution that aerobic nitrate respiration makes to the environmental nitrogen cycle has not been studied. As a first step in addressing this question, a strategy which allows for the isolation of organisms capable of reducing nitrate to nitrite following aerobic growth has been developed. Twenty-nine such strains have been isolated from three soils and a freshwater sediment and shown to comprise members of three genera (Pseudomonas, Aeromonas, and Moraxella). All of these strains expressed a nitrate reductase with an active site located in the periplasmic compartment. Twenty-two of the strains showed significant rates of nitrate respiration in the presence of oxygen when assayed with physiological electron donors. Also isolated was one member of the gram-positive genus Arthrobacter, which was likewise able to respire nitrate in the presence of oxygen but appeared to express a different type of nitrate reductase. In the four environments studied, culturable bacteria capable of aerobic nitrate respiration were isolated in significant numbers (10(4) to 10(7) per g of soil or sediment) and in three cases were as abundant as, or more abundant than, culturable bacteria capable of denitrification. Thus, it seems likely that the corespiration of nitrate and oxygen may indeed make a significant contribution to the flux of nitrate to nitrite in the environment. PMID:7487017

  15. Soil respiration in tropical seasonal forest of Southern Vietnam

    NASA Astrophysics Data System (ADS)

    Avilov, Vitaly; Anichkin, Alexandr; Descherevskaya, Olga; Evdokimova, Elena; Nguyen Van, Thinh; Novichonok, Artyom; Do Phong, Luu; Kurbatova, Julia; Lopes de Gerenyu, Valentin

    2013-04-01

    Soil respiration was monitored as a part of a complex research of carbon balance in Nam Cat Tien National Park in Southern Vietnam (NCT site in AsiaFlux index). The study area is described as a tropical monsoon valley tall-stand forest at altitude about 156 m above sea level, mean annual air temperature is 26.2°C, with fluctuations of monthly averaged temperatures within 4°C; mean annual precipitation is 2470 mm with a distinct alternation of wet and dry seasons (Dong Phu weather station, 1976-1990). Measurements were made every 10-15 days during year 2012 at 6 plots that differ in soil and forest type, mostly in Lagerstroemia- or Dipterocarpus-dominated tree stands. Five chambers Ø162 mm were installed at each plot. CO2 concentration was defined with LI-820 gas analyser and 20 ml syringes (three syringes/samples per chamber) up to August 2012, and by means of closed-loop continuous field analysis from August on. Our studies have shown significant temporal and spatial variability of soil respiration in tropical rainforest. Namely, highest annual CO2 efflux rates were calculated for cambisols under lagerstroemia-dominated tree stand and for light sandy fluvisols under dipterocarpus-dominated tree stand (1694.3±546.0 and 1628.1±442.7 gC•m-2•y-1 respectively). Noteworthy is that the content of organic carbon in these soils varies utterly. Lowest annual CO2 efflux rate was calculated for clay-slate leptosols under dipterocarpus-dominated tree stand (972.7±716.5 gC•m-2•y-1). We also observed a significant impact of termites activity on site-scale spatial variability of soil respiration. Seasonal patterns of soil respiration rates were conformed for all plots except one on sandy soils. The beginning of rainy season in April did not result in higher soil respiration rates, but rates did rise in August - October, at the end of rainy season. Apparently this pattern is related to the accumulation of decomposed organic matter in soil and to the deficient

  16. Species identities, not functional groups, explain the effects of earthworms on litter carbon-derived soil respiration

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Soil respiration is frequently measured as a surrogate for biological activities and is important in soil carbon cycling. The heterotrophic component of soil respiration is primarily driven by microbial decomposition of leaf litter and soil organic matter, and is partially controlled by resource ava...

  17. Soil Respiration in Semiarid Temperate Grasslands under Various Land Management

    PubMed Central

    Hou, Xiangyang; Schellenberg, Michael P.

    2016-01-01

    Soil respiration, a major component of the global carbon cycle, is significantly influenced by land management practices. Grasslands are potentially a major sink for carbon, but can also be a source. Here, we investigated the potential effect of land management (grazing, clipping, and ungrazed enclosures) on soil respiration in the semiarid grassland of northern China. Our results showed the mean soil respiration was significantly higher under enclosures (2.17μmol.m−2.s−1) and clipping (2.06μmol.m−2.s−1) than under grazing (1.65μmol.m−2.s−1) over the three growing seasons. The high rates of soil respiration under enclosure and clipping were associated with the higher belowground net primary productivity (BNPP). Our analyses indicated that soil respiration was primarily related to BNPP under grazing, to soil water content under clipping. Using structural equation models, we found that soil water content, aboveground net primary productivity (ANPP) and BNPP regulated soil respiration, with soil water content as the predominant factor. Our findings highlight that management-induced changes in abiotic (soil temperature and soil water content) and biotic (ANPP and BNPP) factors regulate soil respiration in the semiarid temperate grassland of northern China. PMID:26808376

  18. Soil Respiration in Semiarid Temperate Grasslands under Various Land Management.

    PubMed

    Wang, Zhen; Ji, Lei; Hou, Xiangyang; Schellenberg, Michael P

    2016-01-01

    Soil respiration, a major component of the global carbon cycle, is significantly influenced by land management practices. Grasslands are potentially a major sink for carbon, but can also be a source. Here, we investigated the potential effect of land management (grazing, clipping, and ungrazed enclosures) on soil respiration in the semiarid grassland of northern China. Our results showed the mean soil respiration was significantly higher under enclosures (2.17 μmol.m(-2).s(-1)) and clipping (2.06 μmol.m(-2).s(-1)) than under grazing (1.65 μmol.m-(2).s(-1)) over the three growing seasons. The high rates of soil respiration under enclosure and clipping were associated with the higher belowground net primary productivity (BNPP). Our analyses indicated that soil respiration was primarily related to BNPP under grazing, to soil water content under clipping. Using structural equation models, we found that soil water content, aboveground net primary productivity (ANPP) and BNPP regulated soil respiration, with soil water content as the predominant factor. Our findings highlight that management-induced changes in abiotic (soil temperature and soil water content) and biotic (ANPP and BNPP) factors regulate soil respiration in the semiarid temperate grassland of northern China. PMID:26808376

  19. Forest soil respiration rate and delta13C is regulated by recent above ground weather conditions.

    PubMed

    Ekblad, Alf; Boström, Björn; Holm, Anders; Comstedt, Daniel

    2005-03-01

    Soil respiration, a key component of the global carbon cycle, is a major source of uncertainty when estimating terrestrial carbon budgets at ecosystem and higher levels. Rates of soil and root respiration are assumed to be dependent on soil temperature and soil moisture yet these factors often barely explain half the seasonal variation in soil respiration. We here found that soil moisture (range 16.5-27.6% of dry weight) and soil temperature (range 8-17.5 degrees C) together explained 55% of the variance (cross-validated explained variance; Q2) in soil respiration rate (range 1.0-3.4 micromol C m(-2) s(-1)) in a Norway spruce (Picea abies) forest. We hypothesised that this was due to that the two components of soil respiration, root respiration and decomposition, are governed by different factors. We therefore applied PLS (partial least squares regression) multivariate modelling in which we, together with below ground temperature and soil moisture, used the recent above ground air temperature and air humidity (vapour pressure deficit, VPD) conditions as x-variables. We found that air temperature and VPD data collected 1-4 days before respiration measurements explained 86% of the seasonal variation in the rate of soil respiration. The addition of soil moisture and soil temperature to the PLS-models increased the Q2 to 93%. delta13C analysis of soil respiration supported the hypotheses that there was a fast flux of photosynthates to root respiration and a dependence on recent above ground weather conditions. Taken together, our results suggest that shoot activities the preceding 1-6 days influence, to a large degree, the rate of root and soil respiration. We propose this above ground influence on soil respiration to be proportionally largest in the middle of the growing season and in situations when there is large day-to-day shifts in the above ground weather conditions. During such conditions soil temperature may not exert the major control on root respiration. PMID

  20. Effects of Tillage Practices on Soil Organic Carbon and Soil Respiration

    NASA Astrophysics Data System (ADS)

    Rusu, Teodor; Ioana Moraru, Paula; Bogdan, Ileana; Ioan Pop, Adrian

    2016-04-01

    Soil tillage system and its intensity modify by direct and indirect action soil temperature, moisture, bulk density, porosity, penetration resistance and soil structural condition. Minimum tillage and no-tillage application reduce or completely eliminate the soil mobilization, due to this, soil is compacted in the first years of application. The degree of compaction is directly related to soil type and its state of degradation. All this physicochemical changes affect soil biology and soil respiration. Soil respiration leads to CO2 emissions from soil to the atmosphere, in significant amounts for the global carbon cycle. Soil respiration is one measure of biological activity and decomposition. Soil capacity to produce CO2 varies depending on soil, season, intensity and quality of agrotechnical tillage, soil water, cultivated plant and fertilizer. Our research follows the effects of the three tillage systems: conventional system, minimum tillage and no-tillage on soil respiration and finally on soil organic carbon on rotation soybean - wheat - maize, obtained on an Argic Faeoziom from the Somes Plateau, Romania. To quantify the change in soil respiration under different tillage practices, determinations were made for each crop in four vegetative stages (spring, 5-6 leaves, bean forming, harvest). Soil monitoring system of CO2 and O2 included gradient method, made by using a new generation of sensors capable of measuring CO2 concentration in-situ and quasi-instantaneous in gaseous phase. At surface soil respiration is made by using ACE Automated Soil CO2 Exchange System. These areas were was our research presents a medium multi annual temperature of 8.20C medium of multi annual rain drowns: 613 mm. The experimental variants chosen were: i). Conventional system: reversible plough (22-25 cm) + rotary grape (8-10 cm); ii). Minimum tillage system: paraplow (18-22 cm) + rotary grape (8-10 cm); iii). No-tillage. The experimental design was a split-plot design with three

  1. Seasonal Patterns of Soil Respiration and Related Soil Biochemical Properties under Nitrogen Addition in Winter Wheat Field

    PubMed Central

    Liang, Guopeng; Houssou, Albert A.; Wu, Huijun; Cai, Dianxiong; Wu, Xueping; Gao, Lili; Li, Jing; Wang, Bisheng; Li, Shengping

    2015-01-01

    Understanding the changes of soil respiration under increasing N fertilizer in cropland ecosystems is crucial to accurately predicting global warming. This study explored seasonal variations of soil respiration and its controlling biochemical properties under a gradient of Nitrogen addition during two consecutive winter wheat growing seasons (2013–2015). N was applied at four different levels: 0, 120, 180 and 240 kg N ha-1 year-1 (denoted as N0, N12, N18 and N24, respectively). Soil respiration exhibited significant seasonal variation and was significantly affected by soil temperature with Q10 ranging from 2.04 to 2.46 and from 1.49 to 1.53 during 2013–2014 and 2014–2015 winter wheat growing season, respectively. Soil moisture had no significant effect on soil respiration during 2013–2014 winter wheat growing season but showed a significant and negative correlation with soil respiration during 2014–2015 winter wheat growing season. Soil respiration under N24 treatment was significantly higher than N0 treatment. Averaged over the two growing seasons, N12, N18 and N24 significantly increased soil respiration by 13.4, 16.4 and 25.4% compared with N0, respectively. N addition also significantly increased easily extractable glomalin-related soil protein (EEG), soil organic carbon (SOC), total N, ammonium N and nitrate N contents. In addition, soil respiration was significantly and positively correlated with β-glucosidase activity, EEG, SOC, total N, ammonium N and nitrate N contents. The results indicated that high N fertilization improved soil chemical properties, but significantly increased soil respiration. PMID:26629695

  2. Hydrological controls on heterotrophic soil respiration across an agricultural landscape

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Water availability is an important determinant of variation in soil respiration, but a consistent relationship between soil water and the relative flux rate of carbon dioxide across different soil types remains elusive. Using large undisturbed soil columns (N = 12), we evaluated soil water controls...

  3. Soil Respiration and Organic Carbon Dynamics with Grassland Conversions to Woodlands in Temperate China

    PubMed Central

    Wang, Wei; Zeng, Wenjing; Chen, Weile; Zeng, Hui; Fang, Jingyun

    2013-01-01

    Soils are the largest terrestrial carbon store and soil respiration is the second-largest flux in ecosystem carbon cycling. Across China's temperate region, climatic changes and human activities have frequently caused the transformation of grasslands to woodlands. However, the effect of this transition on soil respiration and soil organic carbon (SOC) dynamics remains uncertain in this area. In this study, we measured in situ soil respiration and SOC storage over a two-year period (Jan. 2007–Dec. 2008) from five characteristic vegetation types in a forest-steppe ecotone of temperate China, including grassland (GR), shrubland (SH), as well as in evergreen coniferous (EC), deciduous coniferous (DC) and deciduous broadleaved forest (DB), to evaluate the changes of soil respiration and SOC storage with grassland conversions to diverse types of woodlands. Annual soil respiration increased by 3%, 6%, 14%, and 22% after the conversion from GR to EC, SH, DC, and DB, respectively. The variation in soil respiration among different vegetation types could be well explained by SOC and soil total nitrogen content. Despite higher soil respiration in woodlands, SOC storage and residence time increased in the upper 20 cm of soil. Our results suggest that the differences in soil environmental conditions, especially soil substrate availability, influenced the level of annual soil respiration produced by different vegetation types. Moreover, shifts from grassland to woody plant dominance resulted in increased SOC storage. Given the widespread increase in woody plant abundance caused by climate change and large-scale afforestation programs, the soils are expected to accumulate and store increased amounts of organic carbon in temperate areas of China. PMID:24058408

  4. Soil respiration and organic carbon dynamics with grassland conversions to woodlands in temperate china.

    PubMed

    Wang, Wei; Zeng, Wenjing; Chen, Weile; Zeng, Hui; Fang, Jingyun

    2013-01-01

    Soils are the largest terrestrial carbon store and soil respiration is the second-largest flux in ecosystem carbon cycling. Across China's temperate region, climatic changes and human activities have frequently caused the transformation of grasslands to woodlands. However, the effect of this transition on soil respiration and soil organic carbon (SOC) dynamics remains uncertain in this area. In this study, we measured in situ soil respiration and SOC storage over a two-year period (Jan. 2007-Dec. 2008) from five characteristic vegetation types in a forest-steppe ecotone of temperate China, including grassland (GR), shrubland (SH), as well as in evergreen coniferous (EC), deciduous coniferous (DC) and deciduous broadleaved forest (DB), to evaluate the changes of soil respiration and SOC storage with grassland conversions to diverse types of woodlands. Annual soil respiration increased by 3%, 6%, 14%, and 22% after the conversion from GR to EC, SH, DC, and DB, respectively. The variation in soil respiration among different vegetation types could be well explained by SOC and soil total nitrogen content. Despite higher soil respiration in woodlands, SOC storage and residence time increased in the upper 20 cm of soil. Our results suggest that the differences in soil environmental conditions, especially soil substrate availability, influenced the level of annual soil respiration produced by different vegetation types. Moreover, shifts from grassland to woody plant dominance resulted in increased SOC storage. Given the widespread increase in woody plant abundance caused by climate change and large-scale afforestation programs, the soils are expected to accumulate and store increased amounts of organic carbon in temperate areas of China. PMID:24058408

  5. Changes in soil respiration components and their specific respiration along three successional forests in the subtropics

    DOE PAGESBeta

    Han, Tianfeng; Liu, Juxiu; Wang, Gangsheng; Huang, Wenjuan; Zhou, Guoyi

    2016-01-16

    1.Understanding how soil respiration components change with forest succession is critical for modelling and predicting soil carbon (C) processes and its sequestration below-ground. The specific respiration (a ratio of respiration to biomass) is increasingly being used as an indicator of forest succession conceptually based on Odum's theory of ecosystem development. However, the hypothesis that specific soil respiration declines with forest succession remains largely untested. 2.We used a trenching method to partition soil respiration into heterotrophic respiration and autotrophic respiration (RH and RA) and then evaluated the specific RH and specific RA in three successional forests in subtropical China. 3.Our resultsmore » showed a clear seasonality in the influence of forest succession on RH, with no significant differences among the three forests in the dry season but a higher value in the old-growth forest than the other two forests in the wet season. RA in the old-growth forest tended to be the highest among the three forests. Both the specific RH and specific RA decreased with the progressive maturity of three forests. 4.Lastly, our results highlight the importance of forest succession in determining the variation of RH in different seasons. With forest succession, soil microbes and plant roots become more efficient to conserve C resources, which would result in a greater proportion of C retained in soils.« less

  6. Partitioning of soil respiration components in a Mediterranean maquis ecosystems

    NASA Astrophysics Data System (ADS)

    Sirca, C.; Carta, M.; Arca, A.; Duce, P.; Spano, D.

    2010-12-01

    Soil respiration is the sum of the CO2 fluxes from the soil produced by the autotrophic component (roots and the associated rhizosphere bacteria and mycorrhizal fungi) and the heterotrophic component (originating from soil micro-organisms that decompose the organic material, usually called basal respiration). Assessing CO2 flux from soil and its relationships with environmental factors is essential to better understand carbon budgets of terrestrial ecosystems. It has been widely recognized the need to improve our knowledge on variations of flux components over time and with climate. Therefore, separate estimations of autotrophic and heterotrophic components are required for analyzing and modeling soil respiration. However, field measurements of soil respiration are difficult. In addition, measurement methods are even more complicated when we attempt to estimate the contribution of the different components to the total CO2 flux (e.g., the trenching method of root and mycorrhizal hyphae exclusion). Moreover, relatively few experiments have been conducted to date on this topic and, to our knowledge, no studies have been focused on Mediterranean ecosystems. With the objective to collect information on soil respiration components in Mediterranean ecosystems, an experiment was set up in July 2008 and is still in progress. Trenching-plot technique and infrared gas exchange analyzer approaches are used for separating and quantifying the soil respiration components in a maquis ecosystem located in Sardinia, Italy. The contributions to the soil respiration by roots, arbuscular mycorrhizal hyphae and microbial organisms are quantified with trenched plot surrounded by a nylon mesh net of 41 and 1 µm and with control plots, where CO2 fluxes, soil moisture content and soil temperature are measured. Preliminary results showed that the three components of soil respiration had similar, coherent seasonal trends. The lowest values were recorded in winter months, and two peaks were

  7. [Differences in soil respiration between cropland and grassland ecosystems and factors influencing soil respiration on the Loess Plateau].

    PubMed

    Zhou, Xiao-Gang; Zhang, Yan-Jun; Nan, Ya-Fang; Liu, Qing-Fang; Guo, Sheng-Li

    2013-03-01

    Understanding the effect of land-use change on soil respiration rates becomes critical in predicting soil carbon cycling under conversion of arable into grassland on the Loess Plateau. From July 2010 to December 2011, CO2 efflux from the soil surface was measured between 08:00 to 10:00 am in clear days by a Licor-8100 closed chamber system (Li-COR, Lincoln, NE, US). Also, soil temperature and soil moisture at the 5-cm depth was measured using a Li-Cor thermocouple and a hand-held frequency-domain reflectometer (ML2x, Delta-T Devices Ltd, UK) at each PVC collar, respectively. We found marked differences (P < 0.05) in soil respiration related to different land-use: the mean cropland soil respiration [1.35 micromol x (m2 x s)(-1)] was 24% (P < 0.05) less than the paired grassland soil respiration [1.67 micromol x (m2 x s)(-1)] (P < 0.05) during the period of experiment and the cumulative CO2-C emissions in grassland (856 g x m(-2)) was 23% (P < 0.05) higher than that in cropland (694 g x m(-2)). Soil moisture from 0-5 cm depth was much drier in cropland and significantly different between cropland and grassland except for winter. However, there were no clear relationships between soil moisture and soil respiration. Soil temperature at 5-cm depth was 2.5 degress C higher in grassland during the period of experiment (P < 0.05). Regression of soil temperature vs. soil respiration indicated significant exponential relationships both in grassland and cropland. Besides, there were intrinsic differences in response of soil respiration to temperature between the cropland and grassland ecosystems: grassland and cropland respiration response was significantly different at the alpha = 0.05 level, also expressed by a higher temperature sensitivity of soil respiration (Q10) in cropland (2.30) relative to grassland (1.74). Soil temperature of cropland and grassland can explain 79% of the variation in the soil respiration in grassland, compared to 82% in cropland. Therefore, land

  8. Simple Laboratory Experiment for Illustrating Soil Respiration.

    ERIC Educational Resources Information Center

    Hattey, J. A.; Johnson, G. V.

    1997-01-01

    Describes an experiment to illustrate the effect of food source and added nutrients (N) on microbial activity in the soil. Supplies include air-dried soil, dried plant material, sources of carbon and nitrogen, a trap such as KOH, colored water, and a 500-mL Erlenmeyer flask. Includes a diagram of an incubation chamber to demonstrate microbial…

  9. Soil Respiration and Bacterial Structure and Function after 17 Years of a Reciprocal Soil Transplant Experiment

    PubMed Central

    Bond-Lamberty, Ben; Bolton, Harvey; Fansler, Sarah; Heredia-Langner, Alejandro; Liu, Chongxuan; McCue, Lee Ann; Bailey, Vanessa

    2016-01-01

    The effects of climate change on soil organic matter—its structure, microbial community, carbon storage, and respiration response—remain uncertain and widely debated. In addition, the effects of climate changes on ecosystem structure and function are often modulated or delayed, meaning that short-term experiments are not sufficient to characterize ecosystem responses. This study capitalized on a long-term reciprocal soil transplant experiment to examine the response of dryland soils to climate change. The two transplant sites were separated by 500 m of elevation on the same mountain slope in eastern Washington state, USA, and had similar plant species and soil types. We resampled the original 1994 soil transplants and controls, measuring CO2 production, temperature response, enzyme activity, and bacterial community structure after 17 years. Over a laboratory incubation of 100 days, reciprocally transplanted soils respired roughly equal cumulative amounts of carbon as non-transplanted controls from the same site. Soils transplanted from the hot, dry, lower site to the cooler and wetter (difference of -5°C monthly maximum air temperature, +50 mm yr-1 precipitation) upper site exhibited almost no respiratory response to temperature (Q10 of 1.1), but soils originally from the upper, cooler site had generally higher respiration rates. The bacterial community structure of transplants did not differ significantly from that of untransplanted controls, however. Slight differences in local climate between the upper and lower Rattlesnake locations, simulated with environmental control chambers during the incubation, thus prompted significant differences in microbial activity, with no observed change to bacterial structure. These results support the idea that environmental shifts can influence soil C through metabolic changes, and suggest that microbial populations responsible for soil heterotrophic respiration may be constrained in surprising ways, even as shorter- and

  10. Soil Respiration and Bacterial Structure and Function after 17 Years of a Reciprocal Soil Transplant Experiment.

    PubMed

    Bond-Lamberty, Ben; Bolton, Harvey; Fansler, Sarah; Heredia-Langner, Alejandro; Liu, Chongxuan; McCue, Lee Ann; Smith, Jeffrey; Bailey, Vanessa

    2016-01-01

    The effects of climate change on soil organic matter-its structure, microbial community, carbon storage, and respiration response-remain uncertain and widely debated. In addition, the effects of climate changes on ecosystem structure and function are often modulated or delayed, meaning that short-term experiments are not sufficient to characterize ecosystem responses. This study capitalized on a long-term reciprocal soil transplant experiment to examine the response of dryland soils to climate change. The two transplant sites were separated by 500 m of elevation on the same mountain slope in eastern Washington state, USA, and had similar plant species and soil types. We resampled the original 1994 soil transplants and controls, measuring CO2 production, temperature response, enzyme activity, and bacterial community structure after 17 years. Over a laboratory incubation of 100 days, reciprocally transplanted soils respired roughly equal cumulative amounts of carbon as non-transplanted controls from the same site. Soils transplanted from the hot, dry, lower site to the cooler and wetter (difference of -5°C monthly maximum air temperature, +50 mm yr-1 precipitation) upper site exhibited almost no respiratory response to temperature (Q10 of 1.1), but soils originally from the upper, cooler site had generally higher respiration rates. The bacterial community structure of transplants did not differ significantly from that of untransplanted controls, however. Slight differences in local climate between the upper and lower Rattlesnake locations, simulated with environmental control chambers during the incubation, thus prompted significant differences in microbial activity, with no observed change to bacterial structure. These results support the idea that environmental shifts can influence soil C through metabolic changes, and suggest that microbial populations responsible for soil heterotrophic respiration may be constrained in surprising ways, even as shorter- and

  11. Soil respiration and bacterial structure and function after 17 years of a reciprocal soil transplant experiment

    DOE PAGESBeta

    Bond-Lamberty, Benjamin; Bolton, Harvey; Fansler, Sarah J.; Heredia-Langner, Alejandro; Liu, Chongxuan; McCue, Lee Ann; Smith, Jeff L.; Bailey, Vanessa L.

    2016-03-02

    The effects of climate change on soil organic matter—its structure, microbial community, carbon storage, and respiration response—remain uncertain and widely debated. In addition, the effects of climate changes on ecosystem structure and function are often modulated or delayed, meaning that short-term experiments are not sufficient to characterize ecosystem responses. This study capitalized on a long-term reciprocal soil transplant experiment to examine the response of dryland soils to climate change. The two transplant sites were separated by 500 m of elevation on the same mountain slope in eastern Washington state, USA, and had similar plant species and soil types. We resampledmore » the original 1994 soil transplants and controls, measuring CO2 production, temperature response, enzyme activity, and bacterial community structure after 17 years. Over a laboratory incubation of 100 days, reciprocally transplanted soils respired roughly equal cumulative amounts of carbon as non-transplanted controls from the same site. Soils transplanted from the hot, dry, lower site to the cooler and wetter (difference of -5 °C monthly maximum air temperature, +50 mm yr-1precipitation) upper site exhibited almost no respiratory response to temperature (Q10 of 1.1), but soils originally from the upper, cooler site had generally higher respiration rates. The bacterial community structure of transplants did not differ significantly from that of untransplanted controls, however. Slight differences in local climate between the upper and lower Rattlesnake locations, simulated with environmental control chambers during the incubation, thus prompted significant differences in microbial activity, with no observed change to bacterial structure. Lastly, these results support the idea that environmental shifts can influence soil C through metabolic changes, and suggest that microbial populations responsible for soil heterotrophic respiration may be constrained in surprising ways, even

  12. Soil microbial respiration from various microhabitats in Arctic landscape: impact of soil type, environmental conditions and soil age

    NASA Astrophysics Data System (ADS)

    Biasi, Christina; Jokinen, Simo; Marushchak, Maija; Trubnikova, Tatiana; Hämäläinen, Kai; Oinonen, Markku; Martikainen, Pertti

    2014-05-01

    methods applied. It seems that the lower decomposability of peat is largely outweighed by higher C stocks at field conditions. Surprisingly, the bare surfaces (peat circles) with 3500 years old C at the surface exhibited about the largest soil microbial respiration rates among all sites as shown by both methods. This is likely due to the immature status of the peat which was during the bulk of its developmental time protected by permafrost, together with high C-densities. The observation is particularly relevant for decomposition of deeper peat at the permafrost-active layer interface in the large vegetated peat plateaus, where soil material similar to the bare surfaces can be found. The results suggest that the chemical nature and high age of the soil SOC in deep peat does not solely guarantee for resistance to decay. Thus, the study highlights risks for potential re-mobilization of C in deep peat soils following thawing. Soil microbial respiration rates need to be better known when predicting the overall carbon sink/source character of tundra ecosystems in a warming climate. Biasi C., Jokinen S., Marushchak M., Hämäläinen K., Trubnikova T., Oinonen M., Martikainen P. (2013). Microbial respiration in Arctic upland and peat soils as source of CO2. Ecosystems. DOI: 10.1007/s10021-013-9710-z.

  13. Soil texture drives responses of soil respiration to precipitation pulses in the sonoran desert: Implications for climate change

    USGS Publications Warehouse

    Cable, J.M.; Ogle, K.; Williams, D.G.; Weltzin, J.F.; Huxman, T. E.

    2008-01-01

    Climate change predictions for the desert southwestern U.S. are for shifts in precipitation patterns. The impacts of climate change may be significant, because desert soil processes are strongly controlled by precipitation inputs ('pulses') via their effect on soil water availability. This study examined the response of soil respiration-an important biological process that affects soil carbon (C) storage-to variation in pulses representative of climate change scenarios for the Sonoran Desert. Because deserts are mosaics of different plant cover types and soil textures-which create patchiness in soil respiration-we examined how these landscape characteristics interact to affect the response of soil respiration to pulses. Pulses were applied to experimental plots of bare and vegetated soil on contrasting soil textures typical of Sonoran Desert grasslands. The data were analyzed within a Bayesian framework to: (1) determine pulse size and antecedent moisture (soil moisture prior to the pulse) effects on soil respiration, (2) quantify soil texture (coarse vs. fine) and cover type (bare vs. vegetated) effects on the response of soil respiration and its components (plant vs. microbial) to pulses, and (3) explore the relationship between long-term variation in pulse regimes and seasonal soil respiration. Regarding objective (1), larger pulses resulted in higher respiration rates, particularly from vegetated fine-textured soil, and dry antecedent conditions amplified respiration responses to pulses (wet antecedent conditions dampened the pulse response). Regarding (2), autotrophic (plant) activity was a significant source (???60%) of respiration and was more sensitive to pulses on coarse- versus fine-textured soils. The sensitivity of heterotrophic (microbial) respiration to pulses was highly dependent on antecedent soil water. Regarding (3), seasonal soil respiration was predicted to increase with both growing season precipitation and mean pulse size (but only for pulses

  14. Land cover heterogeneity and soil respiration in a west Greenland tundra landscape

    NASA Astrophysics Data System (ADS)

    Bradley-Cook, J. I.; Burzynski, A.; Hammond, C. R.; Virginia, R. A.

    2011-12-01

    Multiple direct and indirect pathways underlie the association between land cover classification, temperature and soil respiration. Temperature is a main control of the biological processes that constitute soil respiration, yet the effect of changing atmospheric temperatures on soil carbon flux is unresolved. This study examines associations amongst land cover, soil carbon characteristics, soil respiration, and temperature in an Arctic tundra landscape in western Greenland. We used a 1.34 meter resolution multi-spectral WorldView2 satellite image to conduct an unsupervised multi-staged ISODATA classification to characterize land cover heterogeneity. The four band image was taken on July 10th, 2010, and captures an 18 km by 15 km area in the vicinity of Kangerlussuaq. The four major terrestrial land cover classes identified were: shrub-dominated, graminoid-dominated, mixed vegetation, and bare soil. The bare soil class was comprised of patches where surface soil has been deflated by wind and ridge-top fellfield. We hypothesize that soil respiration and soil carbon storage are associated with land cover classification and temperature. We set up a hierarchical field sampling design to directly observe spatial variation between and within land cover classes along a 20 km temperature gradient extending west from Russell Glacier on the margin of the Greenland Ice Sheet. We used the land cover classification map and ground verification to select nine sites, each containing patches of the four land cover classes. Within each patch we collected soil samples from a 50 cm pit, quantified vegetation, measured active layer depth and determined landscape characteristics. From a subset of field sites we collected additional 10 cm surface soil samples to estimate soil heterogeneity within patches and measured soil respiration using a LiCor 8100 Infrared Gas Analyzer. Soil respiration rates varied with land cover classes, with values ranging from 0.2 mg C/m^2/hr in the bare soil

  15. Temporal changes of soil respiration under different tree species.

    PubMed

    Akburak, Serdar; Makineci, Ender

    2013-04-01

    Soil respiration rates were measured monthly (from April 2007 to March 2008) under four adjacent coniferous plantation sites [Oriental spruce (Picea orientalis L.), Austrian pine (Pinus nigra Arnold), Turkish fir (Abies bornmulleriana L.), and Scots pine (Pinus sylvestris L.)] and adjacent natural Sessile oak forest (Quercus petraea L.) in Belgrad Forest-Istanbul/Turkey. Also, soil moisture, soil temperature, and fine root biomass were determined to identify the underlying environmental variables among sites which are most likely causing differences in soil respiration. Mean annual soil moisture was determined to be between 6.3 % and 8.1 %, and mean annual temperature ranged from 13.0°C to 14.2°C under all species. Mean annual fine root biomass changed between 368.09 g/m(2) and 883.71 g/m(2) indicating significant differences among species. Except May 2007, monthly soil respiration rates show significantly difference among species. However, focusing on tree species, differences of mean annual respiration rates did not differ significantly. Mean annual soil respiration ranged from 0.56 to 1.09 g C/m(2)/day. The highest rates of soil respiration reached on autumn months and the lowest rates were determined on summer season. Soil temperature, soil moisture, and fine root biomass explain mean annual soil respiration rates at the highest under Austrian pine (R (2) = 0.562) and the lowest (R (2) = 0.223) under Turkish fir. PMID:22828980

  16. Responses of soil respiration and its temperature/moisture sensitivity to precipitation in three subtropical forests in southern China

    NASA Astrophysics Data System (ADS)

    Jiang, H.; Deng, Q.; Zhou, G.; Hui, D.; Zhang, D.; Liu, S.; Chu, G.; Li, J.

    2013-06-01

    Both long-term observation data and model simulations suggest an increasing chance of serious drought in the dry season and extreme flood in the wet season in southern China, yet little is known about how changes in precipitation pattern will affect soil respiration in the region. We conducted a field experiment to study the responses of soil respiration to precipitation manipulations - precipitation exclusion to mimic drought, double precipitation to simulate flood, and ambient precipitation as control (abbr. EP, DP and AP, respectively) - in three subtropical forests in southern China. The three forest sites include Masson pine forest (PF), coniferous and broad-leaved mixed forest (MF) and monsoon evergreen broad-leaved forest (BF). Our observations showed that altered precipitation strongly influenced soil respiration, not only through the well-known direct effects of soil moisture on plant and microbial activities, but also by modification of both moisture and temperature sensitivity of soil respiration. In the dry season, soil respiration and its temperature sensitivity, as well as fine root and soil microbial biomass, showed rising trends with precipitation increases in the three forest sites. Contrarily, the moisture sensitivity of soil respiration decreased with precipitation increases. In the wet season, different treatments showed different effects in three forest sites. The EP treatment decreased fine root biomass, soil microbial biomass, soil respiration and its temperature sensitivity, but enhanced soil moisture sensitivity in all three forest sites. The DP treatment significantly increased soil respiration, fine root and soil microbial biomass in the PF only, and no significant change was found for the soil temperature sensitivity. However, the DP treatment in the MF and BF reduced soil temperature sensitivity significantly in the wet season. Our results indicated that soil respiration would decrease in the three subtropical forests if soil moisture

  17. Soil respiration is not limited by reductions in microbial biomass during long-term soil incubations

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Declining rates of soil respiration are reliably observed during long-term laboratory incubations, but the cause is uncertain. We explored different controls on soil respiration during long-term soil incubations. Following a 707 day incubation (30 C) of soils from cultivated and forested plots at Ke...

  18. ESTIMATING ROOT RESPIRATION IN SPRUCE AND BEECH: DECREASES IN SOIL RESPIRATION FOLLOWING GIRDLING

    EPA Science Inventory

    A study was undertaken to follow seasonal fluxes of CO2 from soil and to estimate the contribution of autotrophic (root + mycorrhizal) to total soil respiration (SR) in a mixed stand of European beech (Fagus sylvatica) and Norway spruce (Picea abies) near Freising, Germany. Matu...

  19. Variation in the temperature sensitivity of heterotrophic soil respiration in response to pulse water events and substrate limitation

    NASA Astrophysics Data System (ADS)

    Oikawa, P.; Grantz, D. A.; Jenerette, D.

    2011-12-01

    Heterotrophic soil respiration is not well constrained in current ecosystem models. In particular, there is difficulty parameterizing the temperature sensitivity of soil respiration, as respiration is known to depend on soil moisture and substrate availability. Davidson et al. (2006) proposed that when substrate concentrations are low, respiration can be nearly temperature-insensitive. Additionally, respiration can be negatively related to temperature if increasing temperature leads to lower diffusivity of substrates in soil. In order to aid the development of process-based models incorporating the temperature sensitivity of soil respiration, our goal is to experimentally test the theoretical relationships between water, temperature and soil respiration as outlined by Davidson et al. (2006). We are currently measuring soil respiration pulse dynamics in a fallow agricultural field in the low desert of California (UC Desert Research and Extension Center, El Centro, CA, USA). Heterotrophic soil respiration is being monitored using a flux-gradient soil respiration system with solid-state CO2 sensors at 3 depths. We are also measuring soil moisture, DOC, NH4, NO3, and temperature at 3 depths. Additional measurements include soil microbial biomass carbon, microbial enzyme activity rates, and microbial metabolic capacity. Soil temperatures at our site range from 25-55°C. We are particularly interested in evaluating high end temperature responses of respiration. We aim to develop a model to predict changes in heterotrophic soil respiration in response to pulses of water, decreasing substrate availability, and deactivation of enzymes at high temperatures. Using an irrigation regime of one water pulse every 2 weeks, we aim to test the following hypotheses: 1) At high temperatures, respiration is negatively related to temperature due to decreased substrate diffusivity 2) Longer lag times between rain pulses and maximum respiration rates are associated with substrate

  20. Impact of environmental factors and biological soil crust types on soil respiration in a desert ecosystem.

    PubMed

    Feng, Wei; Zhang, Yuqing; Jia, Xin; Wu, Bin; Zha, Tianshan; Qin, Shugao; Wang, Ben; Shao, Chenxi; Liu, Jiabin; Fa, Keyu

    2014-01-01

    The responses of soil respiration to environmental conditions have been studied extensively in various ecosystems. However, little is known about the impacts of temperature and moisture on soils respiration under biological soil crusts. In this study, CO2 efflux from biologically-crusted soils was measured continuously with an automated chamber system in Ningxia, northwest China, from June to October 2012. The highest soil respiration was observed in lichen-crusted soil (0.93 ± 0.43 µmol m-2 s-1) and the lowest values in algae-crusted soil (0.73 ± 0.31 µmol m-2 s-1). Over the diurnal scale, soil respiration was highest in the morning whereas soil temperature was highest in the midday, which resulted in diurnal hysteresis between the two variables. In addition, the lag time between soil respiration and soil temperature was negatively correlated with the soil volumetric water content and was reduced as soil water content increased. Over the seasonal scale, daily mean nighttime soil respiration was positively correlated with soil temperature when moisture exceeded 0.075 and 0.085 m3 m-3 in lichen- and moss-crusted soil, respectively. However, moisture did not affect on soil respiration in algae-crusted soil during the study period. Daily mean nighttime soil respiration normalized by soil temperature increased with water content in lichen- and moss-crusted soil. Our results indicated that different types of biological soil crusts could affect response of soil respiration to environmental factors. There is a need to consider the spatial distribution of different types of biological soil crusts and their relative contributions to the total C budgets at the ecosystem or landscape level. PMID:25050837

  1. Effect of biosolid waste compost on soil respiration in salt-affected soils

    NASA Astrophysics Data System (ADS)

    Raya, Silvia; Gómez, Ignacio; García, Fuensanta; Navarro, José; Jordán, Manuel Miguel; Belén Almendro, María; Martín Soriano, José

    2013-04-01

    A great part of mediterranean soils are affected by salinization. This is an important problem in semiarid areas increased by the use of low quality waters, the induced salinization due to high phreatic levels and adverse climatology. Salinization affects 25% of irrigated agriculture, producing important losses on the crops. In this situation, the application of organic matter to the soil is one of the possible solutions to improve their quality. The main objective of this research was to asses the relation between the salinity level (electrical conductivity, EC) in the soil and the response of microbial activity (soil respiration rate) after compost addition. The study was conducted for a year. Soil samples were collected near to an agricultural area in Crevillente and Elche, "El Hondo" Natural Park (Comunidad de Regantes from San Felipe Neri). The experiment was developed to determine and quantify the soil respiration rate in 8 different soils differing in salinity. The assay was done in close pots -in greenhouse conditions- containing soil mixed with different doses of sewage sludge compost (2, 4 and 6%) besides the control. They were maintained at 60% of water holding capacity (WHC). Soil samples were analyzed every four months for a year. The equipment used to estimate the soil respiration was a Bac-Trac and CO2 emitted by the soil biota was measured and quantified by electrical impedance changes. It was observed that the respiration rate increases as the proportion of compost added to each sample increases as well. The EC was incremented in each sampling period from the beginning of the experiment, probably due to the fact that soils were in pots and lixiviation was prevented, so the salts couldńt be lost from soil. Over time the compost has been degraded and, it was more susceptible to be mineralized. Salts were accumulated in the soil. Also it was observed a decrease of microbial activity with the increase of salinity in the soil. Keywords: soil

  2. Management Effects on Soil Respiration in North Carolina Coastal Plain Loblolly Pine Plantations

    NASA Astrophysics Data System (ADS)

    Gavazzi, M.; McNulty, S.; Noormets, A.; Treasure, E.

    2012-12-01

    Loblolly pine is the most widely planted tree for plantation management in the southern US. In the southern coastal plain, where much of the original longleaf pine and bottomland hardwood forests have been converted to loblolly pine plantations, inland areas are commonly characterized by deep organic soils that can store up to 80 kg C m-2. Intensive management activities on these sites disturb the forest floor and soil and their impact on soil respiration rates and long term soil storage capabilities is unclear. We measured soil respiration rates in three loblolly pine plantations being managed with a combination of ditching, bedding, clearcutting, thinning and fertilization. Sites and management regimes represented a wide range of real world conditions found in managed southern US forestry plantations. Soil efflux rates along with soil temperature and moisture were measured throughout the year at four to six plots on each site and best fit relationships were developed. Annual soil respiration rates where modeled using 30-minute soil temperature and moisture measurements recorded at a centralized meteorological station on each site. Soil efflux rates were highly correlated with soil temperature and moisture, but interaction between the two effects was uncommon. Soil temperature was the primary driver of soil respiration rates, but rates were suppressed under high soil moisture content. Modeled annual soil efflux rates were higher the first two years following clearcut harvest and thinning operations, but lower two years following fertilization. Rates were lower in the gaps, where entire tree rows were removed, compared to thinned areas, especially on the unfertilized site. Results indicate that soil respiration rates can be strongly impacted by forest management practices; however, the period of increased soil CO2 efflux due to site disturbance may last only a few years.

  3. Impact of Land Use on Soil Respiration in Southwestern Victoria

    NASA Astrophysics Data System (ADS)

    Teodosio, B.; Daly, E.; Pauwels, V. R. N.

    2015-12-01

    Land use management is one of the key contributors to the global environmental change. Considerable changes in landscapes have been experienced in Southwestern Victoria, Australia in the past two decades. Eucalyptus globulus (blue gum) plantations have expanded, resulting in possible changes in the water and carbon balances of catchments. The shift from pastures to plantations could have a significant impact on the local carbon balance with possible effects on atmospheric CO2 concentration and vegetation productivity. We present preliminary measurements from a field study comparing soil respiration in a plantation and a pasture. Adjacent catchments in Southwestern Victoria, near Gatum, were used as study areas; the prominent difference between the two catchments is the land use, with one catchment being used as a pasture for livestock grazing and the other catchment being mainly planted with blue gums. The variability of soil respiration in the pasture is governed by differences in soil moisture and substrate content due to local features of the topography and livestock grazing. Soil respiration measurements in the plantation were taken on mounds, access tracks, and open spaces. Most observations on mounds had higher soil respiration possibly due to root and mycorrhizal respiration. The measurements in open spaces had comparable values with mound measurements; this might be due to a less limited radiation. The soil respiration between trees had lower values, possibly because of radiation limitation due to the canopy cover. These preliminary measurements allow us to compare soil respiration variability across catchments with different land uses. This is important to estimate CO2 fluxes from soil to the atmosphere in large areas and will be valuable in estimating gross primary production from measurements of net ecosystem exchange.

  4. The Effects of Long Term Nitrogen Fertilization on Soil Respiration in Rocky Mountain National Park

    NASA Astrophysics Data System (ADS)

    Allen, J.; Denning, S.; Baron, J.

    2015-12-01

    Anthropogenic activities contribute to increased levels of nitrogen deposition and elevated CO2 concentrations in terrestrial ecosystems. The role that soils play in biogeochemical cycles is an important area of uncertainty in ecosystem ecology. One of the main reasons for this uncertainty is that we have limited understanding of belowground microbial activity and how this activity is linked to soil processes. In particular, elevated CO2 may influence soil nitrogen processes that regulate nitrogen availability to plants. Warming and nitrogen fertilization may both contribute to loss of stored carbon from mountain ecosystems, because they contribute to microbial decomposition of organic matter. To study the effects of long-term nitrogen fertilization on soil respiration, we analyzed results from a 25-year field experiment in Rocky Mountain National Park. Field treatments are in old growth Engelmann spruce forests. Soil respiration responses to the effects of nitrogen fertilization on soil carbon cycling, via respiration, were investigated during the 2013 growing season. Soil moisture, temperature, and respiration rates were measured in six 30 x 30 m plots, of the six plots three are fertilized with 25 kg N ha-1 yr-1 as ammonium nitrate (NH4NO3) pellets and three receives ambient atmospheric nitrogen deposition (1-6 kg N/ha/yr) in Rocky Mountain National Park. We found that respirations rates in the fertilized plots were not significantly higher than respiration rates in the unfertilized plots. We speculate that acclimation to long-term fertilization and relatively high levels of nitrogen deposition in the control plots both contribute to the insensitivity of soil respiration to fertilization at this site.

  5. Methodology for assessing respiration and cellular incorporation of radiolabeled substrates by soil microbial communities (journal version)

    SciTech Connect

    Dobbins, D.C.; Pfaender, F.K.

    1988-01-01

    A method is described for determining biodegradation kinetics of both naturally occurring and xenobiotic compounds in surface and subsurface soil samples. The method measures both respiration and uptake into cellular biomass of 14C-labeled substrates. After separation of the cells and the soil particles by centrifugation, the cells were trapped on membrane filters for liquid scintillation counting. Mass balances were easily obtained. The technique was used to measure metabolic activity in soil profiles, including unsaturated and saturated zones. First-order rate constants were determined for amino acid metabolism and for m-cresol metabolism. Saturation kinetics were observed for amino acids and m-cresol. m-Cresol values for uptake often exceeded those for respiration by greater than a factor of ten. Saturation was not observed in many horizons. Frequently, respiration obeyed saturation kinetics, whereas uptake was first order. It is concluded that measuring only kinetics of respiration may lead to severe underestimations of biodegradation rates.

  6. MEASUREMENT OF SOIL RESPIRATION IN SITU: CHAMBER TECHNIQUES.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Chamber methods depend exclusively on headspace gas concentration measurements or determining the unused capacity of a known chemical trap, they provide only an indirect measure of the CO2 flux across the soil surface, which is in turn equal to the soil respiration rate only under steady-state condi...

  7. Sub-10-Micron and Respirable Particles in Lunar Soils

    NASA Astrophysics Data System (ADS)

    Cooper, B. L.; McKay, D. S.; Riofrio, L. M.; Taylor, L. A.; Gonzalez, C. P.

    2010-03-01

    Grain size analyses of Apollo 11 soil 10084 by a laser diffraction technique shows that this soil contains roughly 2% by volume in the respirable (2.5 µm and below) grain size, in agreement with our prior estimates based on extrapolation of sieve data.

  8. [Factors influencing the spatial variability in soil respiration under different land use regimes].

    PubMed

    Chen, Shu-Tao; Liu, Qiao-Hui; Hu, Zheng-Hua; Liu, Yan; Ren, Jing-Quan; Xie, Wei

    2013-03-01

    In order to investigate the factors influencing the spatial variability in soil respiration under different land use regimes, field experiments were performed. Soil respiration and relevant environment, vegetation and soil factors were measured. The spatial variability in soil respiration and the relationship between soil respiration and these measured factors were investigated. Results indicated that land use regimes had significant effects on soil respiration. Soil respiration varied significantly (P < 0.001) among different land use regimes. Soil respiration rates ranged from 1.82 to 7.46 micromol x (m2 x s)(-1), with a difference of 5.62 micromol x (m2 x s)(-1) between the highest and lowest respiration rates. Soil organic carbon was a key factor controlling the spatial variability in soil respiration. In all, ecosystems studied, the relationship between soil respiration and soil organic carbon content can be described by a power function. Soil respiration increased with the increase of soil organic carbon. In forest ecosystem, the relationship between soil respiration and diameter at breast height (DBH) of trees can be explained by a natural logarithmic function. A model composed of soil organic carbon (C, %), available phosphorous (AP, g x kg(-1)) and diameter at breast height (DBH, cm) explained 92.8% spatial variability in soil respiration for forest ecosystems. PMID:23745410

  9. Autotrophic component of soil respiration is repressed by drought more than the heterotrophic one in a dry grassland

    NASA Astrophysics Data System (ADS)

    Balogh, J.; Papp, M.; Pintér, K.; Fóti, Sz.; Posta, K.; Eugster, W.; Nagy, Z.

    2015-10-01

    Summer droughts projected to increase in Central Europe due to climate change strongly influence the carbon cycle of ecosystems. Persistent respiration activities during drought periods are responsible for a significant carbon loss, which may turn the ecosystem from sink to source of carbon. There are still gaps in our knowledge regarding the characteristic changes taking place in the respiration of the different components of the ecosystem respiration in response to drought events. Here, we combined a physical separation of soil respiration components with continuous measurements of soil CO2 efflux and its isotopic (13C) signal at a dry grassland site in Hungary. The physical separation of soil respiration components was achieved by the use of inox meshes and tubes inserted into the soil. The root-excluded and root- and mycorrhiza-excluded treatments served to measure the isotopic signal of the rhizospheric, mycorrhizal fungi and heterotrophic components, respectively. In the dry grassland investigated in this study the three components of the soil CO2 efflux decreased at different rates under drought conditions. During drought the contribution made by the heterotrophic components was the highest. Rhizospheric component was the most sensitive to soil drying with its relative contribution to the total soil respiration dropping from 71 ± 4 % (non-stressed) to 36 ± 12 % under drought conditions. According to our results, the heterotrophic component of soil respiration is the major contributor to the respiration activities during drought events.

  10. Soil Respiration Declines Following Beetle - Induced Forest Mortality in a Lodgepole Pine Forest

    NASA Astrophysics Data System (ADS)

    Borkhuu, B.; Peckham, S. D.; Norton, U.; Ewers, B. E.; Pendall, E.

    2014-12-01

    Lodgepole pine (Pinus contorta var. latifolia) forests in northern Colorado and southeast Wyoming have been undergoing a major mortality event owing to mountain pine beetle (Dendroctonus ponderosae) infestation since 2007. We studied biotic and abiotic drivers of growing season soil respiration in four mature stands experiencing different levels of mortality between 2008 and 2012 in the Medicine Bow Mountains, southeastern Wyoming, USA. For five years, beetle infestation significantly altered forest structure. Stand mortality was 30% and more than 80% in stands with the lowest and highest mortality, respectively. Understory vegetation cover increased by 50% for five years following beetle infestation. Needlefall was increased by more than 50% during first two years of beetle infestation compared to the pre-disturbance period. We did not observe an immediate increase in soil respiration following beetle infestation as suggested by some researchers. Soil respiration rates in midsummer ranged from 1.4 ± 0.1 μmol m-2 s-1 in stands with highest mortality to 3.1 ± 0.2 μmol m-2s-1 in uninfested stand. Live tree basal area was the dominant factor controlling soil respiration, explaining more than 60% of the interannual and spatial variations in response to the disturbance. In addition, soil respiration was significantly correlated with fine root biomass, which explained 55% of variations, providing strong evidence that autotrophic respiration dominated the forest soil respiration flux. Furthermore, the seasonality of soil respiration was controlled mainly by mean monthly precipitation and mid-day photosynthetically active radiation. Each factor predicted from 30% to 50% of seasonal soil respiration variability with the highest correlation coefficients in stand with the lowest mortality. Our results clearly indicate that the reduction of photosynthesis in trees over the infestation period significantly reduced soil respiration. The remaining activity in dead stands may

  11. Sensing winter soil respiration dynamics in near-real time

    NASA Astrophysics Data System (ADS)

    Contosta, A.; Burakowski, E. A.; Varner, R. K.; Frey, S. D.

    2014-12-01

    Some of the largest reductions in seasonal snow cover are projected to occur in temperate latitudes. Limited measurements from these ecosystems indicate that winter soil respiration releases as much as 30% of carbon fixed during the previous growing season. This respiration is possible with a snowpack that insulates soil from ambient fluctuations in climate. However, relationships among snowpack, soil temperature, soil moisture, and winter soil respiration in temperate regions are not well-understood. Most studies have infrequently sampled soil respiration and its drivers, and most measurements have been limited to the soil surface. We made near-real time, continuous measurements of temperature, moisture, and CO2 fluxes from the soil profile, through the snowpack, and into the atmosphere in a deciduous forest of New Hampshire, USA. We coupled these data with daily sampling of snow depth and snow water equivalent (SWE). Our objectives were to continuously measure soil CO2 production (Psoil) and CO2 flux through the snowpack (Fsnow) and to compare Fsnow and Psoil with environmental drivers. We found that Fsnow was more dynamic than Psoil, changing as much as 30% over several days with shifting environmental conditions. Multiple regression indicated that SWE, air temperature, surface soil temperature, surface soil CO2 concentrations, and soil moisture at 15 cm were significant predictors of Fsnow. The transition of surface temperature from below to above 0°C was particularly important as it represented a phase change from ice to liquid water. Only air temperature and soil moisture at 15 cm were significant drivers of Psoil, where higher moisture at 15 cm resulted in lower Psoil rates. Time series analysis showed that Fsnow lagged 40 days behind Psoil. This lag may be due to slow CO2 diffusion through soil to overlying snow under high moisture conditions. Our results suggest that surface soil CO2 losses are driven by rapid changes in snow cover, surface temperature

  12. Plutonium hazard in respirable dust on the surface of soil.

    PubMed

    Johnson, C J; Tidball, R R; Severson, R C

    1976-08-01

    Plutonium-239 in the fine particulate soil fraction of surface dust is subject to suspension by air currents and is a potential health hazard to humans who may inhale it. This respirable particulate fraction is defined as particles less than or equal to 5 micrometers. The respirable fraction of surface dust was separated by ultrasonic dispersion and a standard water-sedimentation procedure. Plutonium concentration in this fraction of off-site soils located downwind from the Rocky Flats Nuclear Weapons Plant (Jefferson County, Colorado) were as much as 380 times the background concentration. It is prposed that this method of evaluation defines more precisely the potential health hazard from the respirable fraction of plutonium-contaminated soils. PMID:941018

  13. Impact of some selected insecticides application on soil microbial respiration.

    PubMed

    Latif, M A; Razzaque, M A; Rahman, M M

    2008-08-15

    The aim of present study was to investigate the impact of selected insecticides used for controlling brinjal shoot and fruit borer on soil microorganisms and to find out the insecticides or nontoxic to soil microorganism the impact of nine selected insecticides on soil microbial respiration was studied in the laboratory. After injection of different insecticides solutions, the soil was incubated in the laboratory at room temperature for 32 days. The amount of CO2 evolved due to soil microbial respiration was determined at 2, 4, 8, 16, 24 and 32 days of incubation. Flubendiamide, nimbicidine, lambda-cyhalothrin, abamectin and thiodicarb had stimulatory effect on microbial respiration during the initial period of incubation. Chlorpyriphos, cartap and carbosulfan had inhibitory effect on microbial respiration and cypermethrin had no remarkable effect during the early stage of incubation. The negative effect of chlorpyriphos, cartap and carbosulfan was temporary, which was disappeared after 4 days of insecticides application. No effect of the selected insecticides on soil microorganisms was observed after 24 or 32 days of incubation. PMID:19266909

  14. Comparative Assessment of the Effect of Synthetic and Natural Fungicides on Soil Respiration

    PubMed Central

    Stefani, Angelo; Felício, Joanna D’Arc; de Andréa, Mara M.

    2012-01-01

    As toxic pesticide residues may persist in agricultural soils and cause environmental pollution, research on natural fungicides to replace the synthetic compounds is currently increasing. The effect of the synthetic fungicide chlorothalonil and a natural potential fungicide on the soil microbial activity was evaluated here by the substrate-induced respiration by addition of glucose (SIR), as bioindicator in two soils (Eutrophic Humic Gley—GHE and Typic Eutroferric Chernosol—AVEC). The induced soil respiration parameter was followed during 28 days after soil treatment either with chlorathalonil (11 μg·g−1), or the methanolic fraction from Polymnia sonchifolia extraction (300 μg·g−1), and 14C-glucose (4.0 mg and 5.18 Bq of 14C-glucose g−1). The 14C-CO2 produced by the microbial respiration was trapped in NaOH (0.1 M) which was changed each two hours during the first 10 h, and 1, 3, 5, 7, 14 and 28 days after the treatments. The methanolic fraction of the plant extract inhibited (2.2%) and stimulated (1.8%) the respiration of GHE and AVEC, respectively, but the synthetic chlorothalonil caused 16.4% and 2.6% inhibition of the respiration, respectively of the GHE and AVEC soils. As the effects of the natural product were statistically small, this bioindicator indicates that the methanolic fraction of the Polymnia sonchifolia extract, which has fungicide properties, has no environmental effects. PMID:22737005

  15. Response of soil respiration to acid rain in forests of different maturity in southern China.

    PubMed

    Liang, Guohua; Liu, Xingzhao; Chen, Xiaomei; Qiu, Qingyan; Zhang, Deqiang; Chu, Guowei; Liu, Juxiu; Liu, Shizhong; Zhou, Guoyi

    2013-01-01

    The response of soil respiration to acid rain in forests, especially in forests of different maturity, is poorly understood in southern China despite the fact that acid rain has become a serious environmental threat in this region in recent years. Here, we investigated this issue in three subtropical forests of different maturity [i.e. a young pine forest (PF), a transitional mixed conifer and broadleaf forest (MF) and an old-growth broadleaved forest (BF)] in southern China. Soil respiration was measured over two years under four simulated acid rain (SAR) treatments (CK, the local lake water, pH 4.5; T1, water pH 4.0; T2, water pH 3.5; and T3, water pH 3.0). Results indicated that SAR did not significantly affect soil respiration in the PF, whereas it significantly reduced soil respiration in the MF and the BF. The depressed effects on both forests occurred mostly in the warm-wet seasons and were correlated with a decrease in soil microbial activity and in fine root biomass caused by soil acidification under SAR. The sensitivity of the response of soil respiration to SAR showed an increasing trend with the progressive maturity of the three forests, which may result from their differences in acid buffering ability in soil and in litter layer. These results indicated that the depressed effect of acid rain on soil respiration in southern China may be more pronounced in the future in light of the projected change in forest maturity. However, due to the nature of this field study with chronosequence design and the related pseudoreplication for forest types, this inference should be read with caution. Further studies are needed to draw rigorous conclusions regarding the response differences among forests of different maturity using replicated forest types. PMID:23626790

  16. Response of Soil Respiration to Acid Rain in Forests of Different Maturity in Southern China

    PubMed Central

    Chen, Xiaomei; Qiu, Qingyan; Zhang, Deqiang; Chu, Guowei; Liu, Juxiu; Liu, Shizhong; Zhou, Guoyi

    2013-01-01

    The response of soil respiration to acid rain in forests, especially in forests of different maturity, is poorly understood in southern China despite the fact that acid rain has become a serious environmental threat in this region in recent years. Here, we investigated this issue in three subtropical forests of different maturity [i.e. a young pine forest (PF), a transitional mixed conifer and broadleaf forest (MF) and an old-growth broadleaved forest (BF)] in southern China. Soil respiration was measured over two years under four simulated acid rain (SAR) treatments (CK, the local lake water, pH 4.5; T1, water pH 4.0; T2, water pH 3.5; and T3, water pH 3.0). Results indicated that SAR did not significantly affect soil respiration in the PF, whereas it significantly reduced soil respiration in the MF and the BF. The depressed effects on both forests occurred mostly in the warm-wet seasons and were correlated with a decrease in soil microbial activity and in fine root biomass caused by soil acidification under SAR. The sensitivity of the response of soil respiration to SAR showed an increasing trend with the progressive maturity of the three forests, which may result from their differences in acid buffering ability in soil and in litter layer. These results indicated that the depressed effect of acid rain on soil respiration in southern China may be more pronounced in the future in light of the projected change in forest maturity. However, due to the nature of this field study with chronosequence design and the related pseudoreplication for forest types, this inference should be read with caution. Further studies are needed to draw rigorous conclusions regarding the response differences among forests of different maturity using replicated forest types. PMID:23626790

  17. Temperature, Pulse, and Respiration. Learning Activity Package.

    ERIC Educational Resources Information Center

    Runge, Lillian

    This learning activity package on temperature, pulse, and respiration is one of a series of 12 titles developed for use in health occupations education programs. Materials in the package include objectives, a list of materials needed, information sheets, reviews (self evaluations) of portions of the content, and answers to reviews. These topics…

  18. Diffusive fractionation complicates isotopic partitioning of autotrophic and heterotrophic sources of soil respiration.

    PubMed

    Moyes, Andrew B; Gaines, Sarah J; Siegwolf, Rolf T W; Bowling, David R

    2010-11-01

    Carbon isotope ratios (δ¹³C) of heterotrophic and rhizospheric sources of soil respiration under deciduous trees were evaluated over two growing seasons. Fluxes and δ¹³C of soil respiratory CO₂ on trenched and untrenched plots were calculated from closed chambers, profiles of soil CO₂ mole fraction and δ¹³C and continuous open chambers. δ¹³C of respired CO₂ and bulk carbon were measured from excised leaves and roots and sieved soil cores. Large diel variations (>5‰) in δ¹³C of soil respiration were observed when diel flux variability was large relative to average daily fluxes, independent of trenching. Soil gas transport modelling supported the conclusion that diel surface flux δ¹³C variation was driven by non-steady state gas transport effects. Active roots were associated with high summertime soil respiration rates and around 1‰ enrichment in the daily average δ¹³C of the soil surface CO₂ flux. Seasonal δ¹³C variability of about 4‰ (most enriched in summer) was observed on all plots and attributed to the heterotrophic CO₂ source. PMID:20545887

  19. Short term effects of fire on soil respiration in Peruvian Amazon

    NASA Astrophysics Data System (ADS)

    Suarez, L. F.; Kruijt, B.

    2008-05-01

    Severe changes are affecting the role of Amazon in the Earth system. One of these possible effects could be the modification of the role of soils in the carbon cycle due to land use and land cover change activities mainly involving the change of forest by crops. In this sense, fire is the main tool used by farmers for land use and also is an important factor for mobilizing C from the soil to the atmosphere, mainly as CO2. This could have an important effect in the global warming. This proposal will evaluate the variation of the soil respiration related to the seasonality and the fire effects on soils in the Amazon of Peru and Brazil. In experimental locations of Peru with different vegetation cover (forest and pasture), we measured soil respiration along with the organic carbon and the microbial biomass of soils during campaigns covering wet and dry seasons. Complementary measurements of soil temperature, water and nutrient content were performed. Also, we reproduced a fire experiment simulating agricultural local activity by the technique of "slash and burn" to evaluate fire effects on soil respiration. Measurements were taken after the soil cooled and at least 3 days after the fire. Additionally, the carbon stocks of the subplots were evaluated. Evaluation of the variations of CO2 fluxes and the capacity of adaptation to fire and water content are discussed through the comparisons of the different locations, type of soils and concentration of available N (nitrate and ammonium) as an indicator of nutrient content.

  20. Effect of biochar produced at different pyrolysis temperature on the soil respiration of abandoned mine soil

    NASA Astrophysics Data System (ADS)

    Kim, Yong Seong; Kim, Juhee; Hwang, Wonjae; Hyun, Seunghun

    2015-04-01

    Contaminated soils near an abandoned mine site included the high acidic mine tailing have received great interest due to potential risk to human health, because leachable elements in low pH continuously release from mine site soil with ground water and precipitation event. Biochar, which is the obtained pyrolysis process of biomass, is used as a soil amendments and carbon storage. Especially, many researchers report that the biochar application to soil show increasing soil pH, CEC, adsorption capacity of various elements, as well as, enhanced microbial activity. Therefore, biochar application to contaminated soil near abandoned mine site is expected to have a positive effects on management of these site and soils through the decreased leachability of contaminants. However, effects of biochar application to these site on the soil respiration, as a common measure of soil health, are poorly understood. The objective of this study is to evaluate the effects of biochar application to abandoned mine site soil on the microbial activity with soil respiration test. Biochar was obtained from giant Miscanthus in a slow pyrolysis process (heating rate of 10° C min-1 and N2 gas flow rate of 1.2 L min-1) at the temperature of 400° C (BC4) and 700° C (BC7), respectively. All biochar samples were prepared with grinding and sieving for particle size control (150~500μm). Soil sample was collected from abandoned mine site at Korea (36° 58'N, 128° 10'E). Main contaminants of this soil were As (12.5 g kg-1), Pb (7.3 g kg-1), and Zn (1.1 g kg-1). Biochars were applied (5% by dry weight) to the soil (final mixture weight were 800g), and then moisture contents were adjusted to 100% field capacity (-0.33 bar) in the respirometer with vacuum pump. CO2 efflux of each samples was continuously assessed using continuous aeration system (air flow rate 25 cc min-1) using air cylinder during 130hr (at 20° C and darkness condition). The CO2 emitted from the samples were carried to the

  1. Soil Respiration of Three Mangrove Forests on Sanibel Island, Florida

    NASA Astrophysics Data System (ADS)

    Cartwright, F.; Bovard, B. D.

    2011-12-01

    Carbon cycling studies conducted in mangrove forests have typically focused on aboveground processes. Our understanding of carbon storage in these systems is therefore limited by the lack information on belowground processes such as fine root production and soil respiration. To our knowledge there exist no studies investigating temporal patterns in and environmental controls on soil respiration in multiple types of mangrove ecosystems concurrently. This study is part of a larger study on carbon storage in three mangrove forests on Sanibel Island, Florida. Here we report on eight months of soil respiration data within these forests that will ultimately be incorporated into an annual carbon budget for each habitat type. Soil respiration was monitored in the following three mangrove habitat types: a fringe mangrove forest dominated by Rhizophora mangle, a basin mangrove forest dominated by Avicennia germinans, and a higher elevation forest comprised of a mix of Avicennia germinans and Laguncularia racemosa, and non-woody salt marsh species. Beginning in June of 2010, we measured soil emissions of carbon dioxide at 5 random locations within three-100 m2 plots within each habitat type. Sampling was performed at monthly intervals and conducted over the course of three days. For each day, one plot from each habitat type was measured. In addition to soil respiration, soil temperature, salinity and gravimetric moisture content were also measured. Our data indicate the Black mangrove forest, dominated by Avicennia germinans, experiences the highest rates of soil respiration with a mean rate of 4.61 ± 0.60 μmol CO2 m-2 s-1. The mixed mangrove and salt marsh habitat has the lowest soil carbon emission rates with a mean of 2.78 ± 0.40 μmol CO2 m-2 s-1. Soil carbon effluxes appear to peak in the early part of the wet season around May to June and are lower and relatively constant the remainder of the year. Our data also suggest there are important but brief periods where

  2. [Effects of antimicrobial drugs on soil microbial respiration].

    PubMed

    Liu, Feng; Ying, Guang-Guo; Zhou, Qi-Xing; Tao, Ran; Su, Hao-Chang; Li, Xu

    2009-05-15

    The effects on soil microbial respiration of sulfonamides, tetracyclines, macrolides and so on were studied using the direct absorption method. The results show sulfamethazine, sulfamethoxazole, chlortetracycline, tetracycline, tylosin and trimethoprim inhibit soil respiration 34.33%, 34.43%, 2.71%, 3.08%, 7.13%, 38.08% respectively. Sulfamethoxazole and trimethoprim have the highest inhibition rates among all the antibiotics. In early incubation period (0-2 d), the concentrations above 10 mg x kg(-1) of sulfamethazine, sulfamethoxazole and trimethoprim remarkably decrease soil CO2 emission. The effects of these antibiotics vary with their concentrations too. Sulfamethoxazole and trimethoprim show good dose-response relationships. According to the standard of pesticide safety evaluation protocol, the six antibiotics pose a little risk to soil microbial environment. PMID:19558090

  3. Gap filling strategies and error in estimating annual soil respiration

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Soil respiration (Rsoil) is one of the largest CO2 fluxes in the global carbon (C) cycle. Estimation of annual Rsoil requires extrapolation of survey measurements or gap-filling of automated records to produce a complete time series. While many gap-filling methodologies have been employed, there is ...

  4. 2 D patterns of soil gas diffusivity , soil respiration, and methane oxidation in a soil profile

    NASA Astrophysics Data System (ADS)

    Maier, Martin; Schack-Kirchner, Helmer; Lang, Friederike

    2015-04-01

    The apparent gas diffusion coefficient in soil (DS) is an important parameter describing soil aeration, which makes it a key parameter for root growth and gas production and consumption. Horizontal homogeneity in soil profiles is assumed in most studies for soil properties - including DS. This assumption, however, is not valid, even in apparently homogeneous soils, as we know from studies using destructive sampling methods. Using destructive methods may allow catching a glimpse, but a large uncertainty remains, since locations between the sampling positions cannot be analyzed, and measurements cannot be repeated. We developed a new method to determine in situ the apparent soil gas diffusion coefficient in order to examine 2 D pattern of DS and methane oxidation in a soil profile. Different tracer gases (SF6, CF4, C2H6) were injected continuously into the subsoil and measured at several locations in the soil profile. These data allow for modelling inversely the 2 D patterns of DS using Finite Element Modeling. The 2D DS patterns were then combined with naturally occurring CH4 and CO2 concentrations sampled at the same locations to derive the 2D pattern of soil respiration and methane oxidation in the soil profile. We show that methane oxidation and soil respiration zones shift within the soil profile while the gas fluxes at the surface remain rather stable during a the 3 week campaign.

  5. Nitrogen-induced reduction in soil respiration of European forests

    NASA Astrophysics Data System (ADS)

    Berridge, Callum; Fleischer, Katrin; Bistinas, Ioannis; Ekici, Altug; Dolman, Albertus

    2014-05-01

    Soil heterotrophic respiration is parameterized in vegetation models as a temperature-dependent decay function, and is usually spatially constant. We test this fundamental assumption with chamber-based observations of the soil carbon efflux along a >1,100km transect throughout European forests, where the latitude is kept constant to control for insolation. We find a modest, but significant, inter-site linear correlation between air temperature and carbon efflux (r2 = 0.32, p=0.02), but not at 5cm depth soil temperature (r2 = -0.02, p=0.4). Average midday respiration increased West-East and correlates well with distance from the coast (r2 = 0.55, p<0.02). Since soil carbon content proved to be a poor explanatory variable (r2=0.04, p=0.5), we turn our attention to nitrogen deposition to find a strong inverse relationship (r2=-0.5, p<0.01; linear model). We are thus able to reject the null hypothesis that there is no spatial variation in soil efflux amongst comparable sites along the same latitude. Critically, we compliment our analysis with independently measured FLUXNET data that accord with our field observations. In both cases, nitrogen is a more reliable predictor of carbon efflux at the inter-site, grid-size scale than temperature. We explain the reduction in soil respiration as a result of nitrogen inhibition of decomposition.

  6. How switches and lags in biophysical regulators affect spatial-temporal variation of soil respiration in an oak-grass savanna

    NASA Astrophysics Data System (ADS)

    Baldocchi, Dennis; Tang, Jianwu; Xu, Liukang

    2006-06-01

    Complex behavior, associated with soil respiration of an oak-grass savanna ecosystem in California, was quantified with continuous measurements of CO2 exchange at two scales (soil and canopy) and with three methods (overstory and understory eddy covariance systems, soil respiration chambers, and a below-ground CO2 flux gradient system). To partition soil respiration into its autotrophic and heterotrophic components, we exploited spatial gradients in the landscape and seasonal variations in rainfall. During the dry summer, heterotrophic respiration was dominant in the senesced grassland area, whereas autotrophic respiration by roots and the feeding of microbes by root exudates was dominant under the trees. A temporal switch in soil respiration occurred in the spring. But the stimulation of root respiration lagged the timing of leaf-out by the trees. Another temporal switch in soil respiration occurred at the start of autumn rains. This switch was induced by the rapid germination of grass seed and new grass growth. Isolated summer rain storms caused a pulse in soil respiration. Such rain events stimulated microbial respiration only; the rain was not sufficient to replenish soil moisture in the root zone or to germinate grass seed. Soil respiration lagged photosynthetic activity on hourly scales. The likely mechanism is the slow translocation of photosynthate to the roots and associated microbes. Another lag occurred on daily scales because of modulations in photosynthesis and stomatal conductance by the passage of dry and humid air masses.

  7. [Effects of soil temperature and moisture on soil respiration in different forest types in Changbai Mountain].

    PubMed

    Wang, Miao; Ji, Lanzhu; Li, Qiurong; Liu, Yanqiu

    2003-08-01

    The effects of soil temperature (0, 5, 15, 25, 35 degrees C) and water content on soil respiration in three forest types in Changbai Mountain were evaluated in laboratory condition. The results indicated that the soil respiration rate was positively correlated to soil temperature from 0 to 35 degrees C and it increased with soil water content from 0.21 to 0.37 kg.kg-1. The soil respiration rate decreased with soil water content when water content was over the range. The result suggested the interactive effects of temperature and water content on soil respiration. There were significant differences in soil respiration among the various forest types and the highest was in broad leaf Korean pine forest, then in erman's birch forest, and it was the lowest in dark coniferous forest. The optimal condition for soil respiration in broad-leaved Korean pine forest was at 35 degrees C under 0.37 kg.kg-1 water content, and it was at 25 degrees C under 0.21 kg.kg-1 in dark coniferous forest and at 35 degrees C under 0.37 kg.kg-1 water content in erman's birch forest. Because the forests of broad leaf Korean pine, dark coniferous and erman's birch are located at various altitudes, the soil temperatures had 4-5 degrees C variation in different forest types during the same period. The soil respiration rates measured in brown pine mountain soil were lower than those in dark brown forest and they were higher in mountain grass forest soil than those in brown pine mountain soil. PMID:14655349

  8. [Response of Soil Respiration and Organic Carbon to Returning of Different Agricultural Straws and Its Mechanism].

    PubMed

    Cao, Zhan-bo; Wang, Lei; Li, Fan; Fu, Xiao-hua; Le, Yi-quan; Wu, Ji-hua; Lu, Bing; Xu, Dian-sheng

    2016-05-15

    Soybean, maize and rice straws were selected as raw materials to study the response of the soil respiration (SR) and soil organic carbon (SOC) to returning of different straws in the Chongming Dongtan area. The results showed that all of SR, SOC and the plant biomass of the lands with returning of different straws were higher than those of the controls. The soil with soybean straw returning possessed the lowest SR and highest SOC among the three kinds of straws, meaning its higher soil organic carbon sequestration capability than corn and maize straws returning. Straw returning significantly enhanced soil dehydrogenase, β-glycosidase activities and microbial biomass, and soil dehydrogenase activity was significantly correlated with soil respiration. The dehydrogenase activity of the soil with soybean straw returning was the lowest, thus, the lowest SR and highest SOC. Soybean straw had the highest cellulose and lignin contents and the lowest N content among the three kinds of straws, resulting in its lowest biodegradability. Therefore, when soybean straw was returned to soil, it was difficult to degrade completely by soil microorganisms, thus the lowest soil microbial activity, eventually leading to the lowest SR and highest SOC. PMID:27506047

  9. Riparian land-use and rehabilitation: impact on organic matter input and soil respiration.

    PubMed

    Oelbermann, Maren; Raimbault, Beverly A; Gordon, A M

    2015-02-01

    Rehabilitated riparian zones in agricultural landscapes enhance environmental integrity and provide environmental services such as carbon (C) sequestration. This study quantified differences in organic matter input, soil biochemical characteristics, and soil respiration in a 25-year-old rehabilitated (RH), grass (GRS), and undisturbed natural forest (UNF) riparian zone. Input from herbaceous vegetation was significantly greater (P < 0.05) in the GRS riparian zone, whereas autumnal litterfall was significantly greater (P < 0.05) in the RH riparian zone. Soil bulk density was significantly greater (P < 0.05) in the RH riparian zone, but its soil chemical characteristics were significantly lower. Soil respiration rates were lowest (P < 0.05) in the UNF (106 C m(-2) h(-1)), followed by the RH (169 mg C m(-2) h(-1)) and GRS (194 C m(-2) h(-1)) riparian zones. Soil respiration rates were significantly different (P < 0.05) among seasons, and were significantly correlated with soil moisture (P < 0.05) and soil temperature (P < 0.05) in all riparian zones. Soil potential microbial activity indicated a significantly different (P < 0.05) response of the microbial metabolic diversity in the RH compared to the GRS and UNF riparian zones, and principle component analysis showed a distinct difference in microbial activity among the riparian land-use systems. Rehabilitating degraded riparian zones with trees rather than GRS is a more effective approach to the long-term mitigation of CO2. Therefore, the protection of existing natural/undisturbed riparian forests in agricultural landscapes is equally important as their rehabilitation with trees, given their higher levels of soil organic C and lower soil respiration rates. PMID:25432450

  10. Riparian Land-Use and Rehabilitation: Impact on Organic Matter Input and Soil Respiration

    NASA Astrophysics Data System (ADS)

    Oelbermann, Maren; Raimbault, Beverly A.

    2015-02-01

    Rehabilitated riparian zones in agricultural landscapes enhance environmental integrity and provide environmental services such as carbon (C) sequestration. This study quantified differences in organic matter input, soil biochemical characteristics, and soil respiration in a 25-year-old rehabilitated (RH), grass (GRS), and undisturbed natural forest (UNF) riparian zone. Input from herbaceous vegetation was significantly greater ( P < 0.05) in the GRS riparian zone, whereas autumnal litterfall was significantly greater ( P < 0.05) in the RH riparian zone. Soil bulk density was significantly greater ( P < 0.05) in the RH riparian zone, but its soil chemical characteristics were significantly lower. Soil respiration rates were lowest ( P < 0.05) in the UNF (106 C m-2 h-1), followed by the RH (169 mg C m-2 h-1) and GRS (194 C m-2 h-1) riparian zones. Soil respiration rates were significantly different ( P < 0.05) among seasons, and were significantly correlated with soil moisture ( P < 0.05) and soil temperature ( P < 0.05) in all riparian zones. Soil potential microbial activity indicated a significantly different ( P < 0.05) response of the microbial metabolic diversity in the RH compared to the GRS and UNF riparian zones, and principle component analysis showed a distinct difference in microbial activity among the riparian land-use systems. Rehabilitating degraded riparian zones with trees rather than GRS is a more effective approach to the long-term mitigation of CO2. Therefore, the protection of existing natural/undisturbed riparian forests in agricultural landscapes is equally important as their rehabilitation with trees, given their higher levels of soil organic C and lower soil respiration rates.

  11. PHYSICOCHEMICAL PROPERTIES AS PREDICTORS OF ORGANIC CHEMICAL EFFECTS ON SOIL MICROBIAL RESPIRATION

    EPA Science Inventory

    Structure-activity analysis was used to evaluate the effects of 19 hazardous organic chemicals on microbial respiration in two slightly acidic soils (a Captina silt loam from Roane County Tennessee, and a McLaurin sandy loam from Stone County, Mississippi), both low in organic ca...

  12. Digging up your dirt. High school students combine small-scale respiration and soil carbon measurements with satellite imagery in hands-on inquiry activities.

    NASA Astrophysics Data System (ADS)

    Kemper, K.; Throop, H.

    2015-12-01

    One of the greatest impacts on the global carbon cycle is changes in land use. Making this concept relevant and inquiry-based for high school students is challenging. Many are familiar with reconstructing paleo-climate from ice core data, but few have a connection to current climate research. Many students ask questions like 'What will our area be like in 20 years?' or 'How much does planting trees help?' while few have the scientific language to engage in a discussion to answer these questions. Our work connects students to climate change research in several ways: first, teacher Keska Kemper engaged in field research with Dr. Heather Throop creating a 'teacher in the field' perspective for students in the classroom. Dr. Throop met with Keska Kemper's students several times to develop an inquiry-based field study. Students predicted and then measured rates of respiration between different soil types in an urban park close to their school. Students then could compare their results from Portland, Oregon to Throop's work across a rain gradient in Australia. Discussions about percent tree cover and soil carbon helped students see connections between land use changes and changes in carbon cycling. Last, students examined satellite imagery to determine percent tree cover and numberss of trees to compare to soil carbon in the same region. Students were able to examine imagery over the last 30 years to visualize land use changes in the greater Portland area.

  13. Temperature-independent diel variation in soil respiration observed from a temperate deciduous forest

    SciTech Connect

    Post, Wilfred M; Liu, Qing; Edwards, Nelson T; Gu, Lianhong; Childs, Joanne; Lenhart, Suzanne M

    2006-01-01

    The response of soil respiration (Rs) to temperature depends largely on the temporal and spatial scales of interest and how other environmental factors interact with this response. They are often represented by empirical exponential equations in many ecosystem analyses because of the difficulties in separating covarying environmental responses and in observing below ground processes. The objective of this study was to quantify a soil temperature-independent component in Rs by examining the diel variation of an Rs time series measured in a temperate deciduous forest located at Oak Ridge, TN, USA between March and December 2003. By fitting 2 hourly, continuous automatic chamber measurements of CO2 efflux at the soil surface to a Q10 function to obtain the temperature-dependent respiration (Rt) and plotting the diel cycles of Rt, Rs, and their difference (Ri), we found that an obvious temperature-independent component exists in Rs during the growing season. The diel cycle of this component has a distinct day/night pattern and agrees well with diel variations in photosynthetically active radiation (PAR) and air temperature. Elevated canopy CO2 concentration resulted in similar patterns in the diel cycle of the temperature-independent component but with different daily average rates in different stages of growing season. We speculate that photosynthesis of the stand is one of the main contributors to this temperature-independent respiration component although more experiments are needed to draw a firm conclusion. We also found that despite its relatively small magnitude compared with the temperature-dependent component, the diel variation in the temperature-independent component can lead to significantly different estimates of the temperature sensitivity of soil respiration in the study forest. As a result, the common practice of using fitted temperature-dependent function from night-time measurements to extrapolate soil respiration during the daytime may underestimate

  14. Effects of endosulfan on soil respiration

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Endosulfan (6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzadioxathiepin 3-oxide) is commonly applied to agricultural crops as a insecticide. However, little is known about its effect on soil microorganisms. A study was conducted to assess the fate and transport of endosulfan...

  15. Amplification and dampening of soil respiration by changes in temperature variability

    USGS Publications Warehouse

    Sierra, C.A.; Harmon, M.E.; Thomann, E.; Perakis, S.S.; Loescher, H.W.

    2011-01-01

    Accelerated release of carbon from soils is one of the most important feed backs related to anthropogenically induced climate change. Studies addressing the mechanisms for soil carbon release through organic matter decomposition have focused on the effect of changes in the average temperature, with little attention to changes in temperature vari-ability. Anthropogenic activities are likely to modify both the average state and the variability of the climatic system; therefore, the effects of future warming on decomposition should not only focus on trends in the average temperature, but also variability expressed as a change of the probability distribution of temperature.Using analytical and numerical analyses we tested common relationships between temperature and respiration and found that the variability of temperature plays an important role determining respiration rates of soil organic matter. Changes in temperature variability, without changes in the average temperature, can affect the amount of carbon released through respiration over the long term. Furthermore, simultaneous changes in the average and variance of temperature can either amplify or dampen there release of carbon through soil respiration as climate regimes change. The effects depend on the degree of convexity of the relationship between temperature and respiration and the magnitude of the change in temperature variance. A potential consequence of this effect of variability would be higher respiration in regions where both the mean and variance of temperature are expected to increase, such as in some low latitude regions; and lower amounts of respiration where the average temperature is expected to increase and the variance to decrease, such as in northern high latitudes.

  16. Estimation of autotrophic soil respiration in a boreal forest using three different approaches

    NASA Astrophysics Data System (ADS)

    Kulmala, Liisa; Pumpanen, Jukka; Heinonsalo, Jussi

    2016-04-01

    It is generally challenging to separate autotrophic and heterotrophic soil respiration. The reason for these difficulties is connected with the intimate interaction of the key processes in soil. Root-associated microbes practically colonize the whole soil volume while decomposition processes occur in the same matrix. Therefore, autotrophic and heterotrophic processes cannot be separated in natural systems. However, there are several methods that can be used to better understand the dynamics of these two. A classical method is called 'trenching' where a trench is dug around a known volume of soil and the roots entering the soil are cut from the living trees thus blocking the C flow from them. The second way to separate autotrophic and heterotrophic respiration relies on the difference in the isotopic signature (13C) of plant-derived or decomposition-derived CO2. The third way to separate the sources is to study the differences in the short- and long-term temperature dependencies in CO2 soil emissions. This is possible especially in boreal forests where the biological activity has a strong seasonal cycle. We compared these three methods in an experiment conducted in a southern boreal middle-aged Scots pine stand in Finland. Our data provides a unique possibility to critically evaluate current methods for estimating autotrophic and heterotrophic soil respiration. The knowledge is needed to study further plant physiology and plant-microbe interactions in soil.

  17. Soil organic matter content: a non-liner control on microbial respiration in soils

    NASA Astrophysics Data System (ADS)

    Schnecker, Jörg; Grandy, Stuart

    2016-04-01

    It is widely assumed that microbial activity and respiration rates respond linearly to substrate concentrations, irrespective of substrate chemical characteristics, but this assumption remains largely untested. We know that microbial decomposition of soil organic matter (SOM) and the amount of CO2 respired from soil depends on substrate availability. While soils with high SOM concentrations will have higher respiration rates than soils with low SOM concentrations, the specific relationship between substrate quantity and CO2 respired and its underlying mechanisms has robust theoretical, modeling, and management implications. In a lab incubation experiment, we amended a mixture of agricultural soil and sand with increasing amounts of one of three plant residues differing in their C/N ratio (clover C/N 14; rye C/N 23 and wheat straw C/N 110). Keeping the soil/sand mixture at a constant ratio, we obtained 9 levels of organic carbon (OC) content ranging from 0.25% to 5.7%. The sand-soil-residue mixtures were then incubated at constant temperature and water contents for a total of 63 days. Our results show that across substrates CO2 production increased with increasing OC content following a sigmoidal curve function instead of the expected linear one. A breakpoint analysis for the respiration curve of rye revealed two significant break points at 1.3 and 3.8 % OC. The three individual linear relations might be shaped by spatial separation of substrate and microbes and the interaction of the microbes themselves. In the first "survival" phase up to 1.3 % OC, more substrate leads to the survival of more microbes. However, microbial growth does not result in the discovery of new resources. In the "expansion" phase (1.3 % OC to 3.8 % OC), microbial growth is successful and microbes can exploit new resources. Finally, in the "competition" phase microbes start to compete for space and resources, which leads to a decrease in decomposition and respiration. While the results for

  18. [Variation characteristic in soil respiration of apple orchard and its biotic and abiotic influencing factors].

    PubMed

    Wang, Rui; Guo, Sheng-Li; Liu, Qing-Fang; Zhang, Yan-Jun; Jiang, Ji-Shao; Guo, Hui-Min; Li, Ru-Jian

    2014-05-01

    To evaluate the orchard variability of soil respiration and the response of soil respiration to its influencing factors is helpful for a deep understanding about the effects of converting cropland to apple orchard. A field experiment was conducted in the Changwu State Key Agro-Ecological Station. Soil respiration, soil temperature, soil moisture and roots biomasses were periodically measured in a mature apple orchard during 2011 and 2012. Soil respiration decreased as the distance from the trunk increased. The cumulative soil respiration in the 0.5 m-distance from the trunk was 20% and 31% higher than that in the 2 m-distance from the trunk, respectively in 2011 and 2012. The temperature sensitivity of soil respiration (Q10) was relatively lower in the 2 m-distance than that in the 0. 5 m-distance in both years. Soil temperature and soil moisture were slightly higher in the 2 m-distance, but there was no significant difference between the 2 m-distance and the 0. 5 m-distance. Soil respiration and soil temperature showed a significant exponential relationship, but there was no positive correlation between soil moisture and soil respiration. Soil temperature changes can explain seasonal variation of soil respiration well, but it could not explain its spatial variability. Root density was an important factor for the spatial variability of soil respiration and Q15. Variation of soil respiration coefficient was 23% -31%. Therefore, the distance from the trunk should be considered when estimating orchards soil respiration. PMID:25055686

  19. Heavy Metal Pollution Enhances Soil Respiration and Reduces Carbon Storage in a Chinese Paddy Soil

    NASA Astrophysics Data System (ADS)

    Pan, Genxing; Li, Zhipeng; Liu, Yongzhuo; Smith, Pete; Crowley, David; Zheng, Jufeng

    2010-05-01

    China's paddy soils are crucial both for food security through high cereal productivity, and for climate mitigation through high soil carbon storage. These functions are increasingly threatened by widespread heavy metal pollution, resulting from rapid industrial development. Heavy metal-polluted soils generally have a reduced microbial biomass and reduced soil respiration, as well as reduced functional diversity through changes in microbial community structure. Here we show that heavy metal pollution enhances soil respiration and CO2 efflux from a Chinese rice paddy soil, and leads to a soil organic carbon (SOC) loss, which is correlated with a decline in the fungal-to-bacterial ratio of the reduced soil microbial community. The pollution-induced SOC loss could offset 70% of the yearly SOC increase from China's paddy soils. Thus, heavy metal pollution impacts long term productivity and the potential for C sequestration in China's paddy soils.

  20. The moisture response of soil heterotrophic respiration: Interaction with soil properties.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Soil moisture-respiration functions are used to simulate the various mechanisms determining the relations between soil moisture content and carbon mineralization. Soil models used in the simulation of global carbon fluxes often apply simplified functions assumed to represent an average moisture-resp...

  1. A global database of soil respiration data

    SciTech Connect

    Bond-Lamberty, Benjamin; Thomson, Allison M.

    2010-06-16

    Soil respiration—RS, the flux of autotropically- and heterotrophically-generated CO2 from the soil to the atmosphere—remains the least well-constrained component of the terrestrial C cycle. Here we introduce the SRDB database, a near-universal compendium of published RS data, and make it available to the scientific community both as a traditional static archive and as a dynamic community database that will be updated over time by interested users. The database encompasses all published studies that report one of the following data measured in the field (not laboratory): annual RS, mean seasonal RS, a seasonal or annual partitioning of RS into its sources fluxes, RS temperature response (Q10), or RS at 10 °C. Its orientation is thus to seasonal and annual fluxes, not shorter-term or chamber-specific measurements. To date, data from 818 studies have been entered into the database, constituting 3379 records. The data span the measurement years 1961-2007 and are dominated by temperate, well-drained forests. We briefly examine some aspects of the SRDB data—mean annual RS fluxes and their correlation with other carbon fluxes, RS variability, temperature sensitivities, and the partitioning of RS source flux–and suggest some potential lines of research that could be explored using these data. The SRDB database described here is available online in a permanent archive as well as via a project-hosting repository; the latter source leverages open-source software technologies to encourage wider participation in the database’s future development. Ultimately, we hope that the updating of, and corrections to, the SRDB will become a shared project, managed by the users of these data in the scientific community.

  2. Soil Respiration in Different Agricultural and Natural Ecosystems in an Arid Region

    PubMed Central

    Lai, Liming; Zhao, Xuechun; Jiang, Lianhe; Wang, Yongji; Luo, Liangguo; Zheng, Yuanrun; Chen, Xi; Rimmington, Glyn M.

    2012-01-01

    The variation of different ecosystems on the terrestrial carbon balance is predicted to be large. We investigated a typical arid region with widespread saline/alkaline soils, and evaluated soil respiration of different agricultural and natural ecosystems. Soil respiration for five ecosystems together with soil temperature, soil moisture, soil pH, soil electric conductivity and soil organic carbon content were investigated in the field. Comparing with the natural ecosystems, the mean seasonal soil respiration rates of the agricultural ecosystems were 96%–386% higher and agricultural ecosystems exhibited lower CO2 absorption by the saline/alkaline soil. Soil temperature and moisture together explained 48%, 86%, 84%, 54% and 54% of the seasonal variations of soil respiration in the five ecosystems, respectively. There was a significant negative relationship between soil respiration and soil electrical conductivity, but a weak correlation between soil respiration and soil pH or soil organic carbon content. Our results showed that soil CO2 emissions were significantly different among different agricultural and natural ecosystems, although we caution that this was an observational, not manipulative, study. Temperature at the soil surface and electric conductivity were the main driving factors of soil respiration across the five ecosystems. Care should be taken when converting native vegetation into cropland from the point of view of greenhouse gas emissions. PMID:23082234

  3. Winter soil respiration from different vegetation patches in the Yellow River Delta, China.

    PubMed

    Han, Guangxuan; Yu, Junbao; Li, Huabing; Yang, Liqiong; Wang, Guangmei; Mao, Peili; Gao, Yongjun

    2012-07-01

    Vegetation type and density exhibited a considerable patchy distribution at very local scales in the Yellow River Delta, due to the spatial variation of soil salinity and water scarcity. We proposed that soil respiration is affected by the spatial variations in vegetation type and soil chemical properties and tested this hypothesis in three different vegetation patches (Phragmites australis, Suaeda heteroptera and bare soil) in winter (from November 2010 to April 2011). At diurnal scale, soil respiration all displayed single-peak curves and asymmetric patterns in the three vegetation patches; At seasonal scale, soil respiration all declined steadily until February, and then increased to a peak in next April. But, the magnitude of soil respiration showed significant differences among the three sites. Mean soil respiration rates in winter were 0.60, 0.45 and 0.17 μmol CO(2) m(-2) s(-1) for the Phragmites australis, Suaeda heteroptera and bare soil, respectively. The combined effect of soil temperature and soil moisture accounted for 58-68 % of the seasonal variation of winter soil respiration. The mean soil respiration revealed positive and linear correlations with total N, total N and SOC storages at 0-20 cm depth, and plant biomass among the three sites. We conclude that the patchy distribution of plant biomass and soil chemical properties (total C, total N and SOC) may affect decomposition rate of soil organic matter in winter, thereby leading to spatial variations in soil respiration. PMID:22576142

  4. [Dynamic changes in soil respiration components and their regulating factors in the Moso bamboo plantation in subtropical China].

    PubMed

    Yang, Wen-jia; Li, Yong-fu; Jiang, Pei-kun; Zhou, Guo-mo; Liu, Juan

    2015-10-01

    Dynamic changes (from April 2013 to March 2014) in soil respiration components were investigated by Li-8100 in the Moso bamboo plantation in Lin' an City, Zhejiang Province. Results showed that the average annual values for the soil total respiration rate, heterotrophic respiration rate, and autotrophic respiration rate in the Moso bamboo plantation were 2.93, 1.92 and 1.01 imol CO2 . m-2 . s-1, respectively. The soil respiration rate and its components exhibited strongly a seasonal dynamic pattern. The maximum appeared in July 2013, and the minimum appeared in January 2014. The annual cumulative CO2 emissions through soil respiration, heterotrophic respiration, and autotrophic respiration were 37.25, 24.61 and 12.64 t CO2 . hm-2 . a-1, respectively. The soil respiration and its components showed a close relation with soil temperature of 5 cm depth, and the corresponding Q10, values at 5 cm depth were 2.05, 1.95 and 2.34, respectively. Both the soil respiration and heterotrophic respiration were correlated to soil water soluble organic C (WSOC) content, but no significant relationship between autotrophic respiration and WSOC was observed. There were no significant relationships between soil respiration components and soil moisture content or microbial biomass C. The seasonal changes in soil respiration components in the Moso bamboo plantation were predominantly controlled by the soil temperature, and the soil WSOC content was an important environmental factor controlling total soil respiration and soil heterotrophic respiration. PMID:26995900

  5. Comparing how land use change impacts soil microbial catabolic respiration in Southwestern Amazon.

    PubMed

    Mazzetto, Andre Mancebo; Feigl, Brigitte Josefine; Cerri, Carlos Eduardo Pellegrino; Cerri, Carlos Clemente

    2016-01-01

    Land use changes strongly impact soil functions, particularly microbial biomass diversity and activity. We hypothesized that the catabolic respiration response of the microbial biomass would differ depending on land use and that these differences would be consistent at the landscape scale. In the present study, we analyzed the catabolic response profile of the soil microbial biomass through substrate-induced respiration in different land uses over a wide geographical range in Mato Grosso and Rondônia state (Southwest Amazon region). We analyzed the differences among native areas, pastures and crop areas and within each land use and examined only native areas (Forest, Dense Cerrado and Cerrado), pastures (Nominal, Degraded and Improved) and crop areas (Perennial, No-Tillage, Conventional Tillage). The metabolic profile of the microbial biomass was accessed using substrate-induced respiration. Pasture soils showed significant responses to amino acids and carboxylic acids, whereas native areas showed higher responses to malonic acid, malic acid and succinic acid. Within each land use category, the catabolic responses showed similar patterns in both large general comparisons (native area, pasture and crop areas) and more specific comparisons (biomes, pastures and crop types). The results showed that the catabolic responses of the microbial biomass are highly correlated with land use, independent of soil type or climate. The substrate induced respiration approach is useful to discriminate microbial communities, even on a large scale. PMID:26887228

  6. Comparing how land use change impacts soil microbial catabolic respiration in Southwestern Amazon

    PubMed Central

    Mazzetto, Andre Mancebo; Feigl, Brigitte Josefine; Cerri, Carlos Eduardo Pellegrino; Cerri, Carlos Clemente

    2016-01-01

    Land use changes strongly impact soil functions, particularly microbial biomass diversity and activity. We hypothesized that the catabolic respiration response of the microbial biomass would differ depending on land use and that these differences would be consistent at the landscape scale. In the present study, we analyzed the catabolic response profile of the soil microbial biomass through substrate-induced respiration in different land uses over a wide geographical range in Mato Grosso and Rondônia state (Southwest Amazon region). We analyzed the differences among native areas, pastures and crop areas and within each land use and examined only native areas (Forest, Dense Cerrado and Cerrado), pastures (Nominal, Degraded and Improved) and crop areas (Perennial, No-Tillage, Conventional Tillage). The metabolic profile of the microbial biomass was accessed using substrate-induced respiration. Pasture soils showed significant responses to amino acids and carboxylic acids, whereas native areas showed higher responses to malonic acid, malic acid and succinic acid. Within each land use category, the catabolic responses showed similar patterns in both large general comparisons (native area, pasture and crop areas) and more specific comparisons (biomes, pastures and crop types). The results showed that the catabolic responses of the microbial biomass are highly correlated with land use, independent of soil type or climate. The substrate induced respiration approach is useful to discriminate microbial communities, even on a large scale. PMID:26887228

  7. Continuous Monitoring of Soil Respiration in Black Spruce Forest Soils, Interior Alaska

    NASA Astrophysics Data System (ADS)

    Kim, Y.; Kim, S.; Kim, W.

    2009-12-01

    This research was carried out to estimate the continuous monitoring of soil respiration using automatic chamber system that was equipped with a control system, a compressor, and seven chambers (50 cm diameter, 30 cm high) set in sphagnum moss, feather moss, lichen, and tussock in black spruce forest soils, interior Alaska during growing season of 2008. The average daily soil respirations were 0.050±0.012 (standard deviation, CV 23%), 0.022±0.020 (91%), 0.082±0.035 (43%), and 0.027±0.010 mgCO2/m2/s (37%) in lichens, sphagnum moss, tussock and feather moss on black spruce forest soils with light chamber made by transparent material. The temporal variation of soil respiration in different vegetation types on black spruce forest soils during the growing season of 2008 is shown in Figure 1. The accumulative daily soil respiration was 5.2, 9.5, 2.3, and 2.8 mgCO2/m2/s in lichen, tussock, sphagnum moss, and feather moss of black spruce forest ground during the growing periods of 103 days, 2008 (Figure 2). Therefore, averaged regional soil respiration rate is 0.19±0.18 and 0.12±0.08 kgC/m2/(growing season) of 2007 and 2008 in black spruce forest soils, interior Alaska. The winter soil respiration was 0.049±0.013 gC/m2/(winter season), corresponding from 21±7% to 29±13% of the annual CO2 emitted from black spruce forest soils, interior Alaska.

  8. Soil Organic Matter Content: A Non-linear Control on Microbial Respiration in Soils

    NASA Astrophysics Data System (ADS)

    Schnecker, J.; Grandy, S.

    2015-12-01

    Decomposition of soil organic matter (SOM) and the amount of CO2 respired from soil largely depends on the amount of substrate available to microbes. Soils with high SOM concentrations will have higher respiration rates than soils with low SOM concentrations given similar environmental conditions. It is widely assumed that microbial activity and respiration rates respond linearly to substrate concentrations. This assumption remains however largely untested. In a lab incubation experiment, we amended a mixture of agricultural soil and sand with increasing amounts of one of three plant residues differing in their C/N ratio (clover 14; rye 23 and wheat straw 110). We used 9 levels of organic carbon (OC) content ranging from 0.25% to 5.7%. The mixtures were then incubated at constant temperature and water contents for 63 days. Our results show that across substrates CO2 production increased with increasing OC content following a quadratic function instead of the expected linear one up to 2.2% OC. Above that point CO2 production leveled off and increased linearly. We hypothesize that the probability that a microbe meets a substrate also increases with increasing amounts of plant residues. At all substrate concentrations, samples amended with clover had the highest carbon losses, followed by rye and straw. Differences between the three kinds of plant residue might have been caused by their C/N ratios and thus the amount of available N. High amounts of N might have led to an increase in microbial biomass, which could occupy more space and is thus more likely to meet new substrate. Additional analysis of microbial biomass, enzyme activities and N pools will help to understand the mechanism leading to the observed CO2 patterns. A non-linear relation of CO2 production and OC content indicates that spatial separation as an inherent property of SOM content is an important control on decomposition at low OC contents. Knowledge of this controlling effect could be used to enhance

  9. Soil respiration in a long-term tillage treatment experiment

    NASA Astrophysics Data System (ADS)

    Gelybó, Györgyi; Birkás, Márta; Dencsö, Márton; Horel, Ágota; Kása, Ilona; Tóth, Eszter

    2016-04-01

    Regular soil CO2 efflux measurements have been carried out at Józsefmajor longterm tillage experimental site in 2014 and 2015 with static chamber technique in no-till and ploughing plots in seven spatial replicates. The trial was established in 2002 on a loamy chernozem soil at the experimental site of the Szent István University nearby the city Hatvan, northern Hungary. At the site sunflower (Helianthus A.) and wheat (Triticum A.) was grown in 2014 and 2015, respectively. Ancillary measurements carried out at the site included weather parameters, soil water content, soil temperature. The aim of the investigation was to detect the effect of soil disturbance and soil tillage treatments on soil CO2 emission in agricultural ecosystems. Soil respiration measurements were carried out every week during the vegetation period and campaign measurements were performed scheduled to tillage application. In this latter case, measurements were carried out 1, 2, 3, 4, 6, 12, 18, 24, 48, 72, 96, 120 hours and 7 days after tillage operation. Results showed that during the vegetation season in the majority of measurement occasions emission was higher in the no-till plots. These differences; however were not found to be statistically significant. Due to the short term effect of tillage treatment, emissions increased following tillage treatment in the ploughed plots. Soil water content was also examined as main driver of soil CO2 fluxes. Soil water content sharply decreases in the surface layer (5-10 cm depth) after tillage treatment indicating a fast drying due to soil disturbance. This effect slowly attenuated and eventually extincted in approx. two weeks. CO2 emission measurements were associated with high uncertainties as a result of the measurement technique. Our further aim is to reduce this uncertainty using independent measurement techniques on the field.

  10. [Effects of Green Manure Intercropping and Straw Mulching on Winter Rape Rhizosphere Soil Organic Carbon and Soil Respiration].

    PubMed

    Zhou, Quan; Wang, Long-chang; Xiong, Ying; Zhang, Sai; Du, Juan; Zhao, Lin-lu

    2016-03-15

    Under the background of global warming, the farmland soil respiration has become the main way of agricultural carbon emissions. And green manure has great potential to curb greenhouse gas emissions and achieve energy conservation and emissions reduction. However, in purple soil region of Southwest, China, soil respiration under green manure remains unclear, especially in the winter and intercropping. Through the green manure ( Chinese milk vetch) intercropping with rape, therefore, we compared the effects of rape rhizosphere under straw mulching. The soil organic carbon and soil respiration were examined. The results showed, compared with straw mulching, root separation was the major influencing factors of soil organic carbon on rape rhizosphere. Soil organic carbon was significantly decreased by root interaction. In addition, straw mulching promoted while green manure intercropping inhibited the soil respiration. Soil respiration presented the general characteristics of fall-rise-fall due to the strong influence of rape growth period. Therefore, it showed a cubic curve relationship with soil temperature. PMID:27337908

  11. Biocrusts modulate warming and rainfall exclusion effects on soil respiration in a semi-arid grassland

    PubMed Central

    Escolar, Cristina; Maestre, Fernando T.; Rey, Ana

    2015-01-01

    Soil surface communities composed of cyanobacteria, algae, mosses, liverworts, fungi, bacteria and lichens (biocrusts) largely affect soil respiration in dryland ecosystems. Climate change is expected to have large effects on biocrusts and associated ecosystem processes. However, few studies so far have experimentally assessed how expected changes in temperature and rainfall will affect soil respiration in biocrust-dominated ecosystems. We evaluated the impacts of biocrust development, increased air temperature and decreased precipitation on soil respiration dynamics during dry (2009) and wet (2010) years, and investigated the relative importance of soil temperature and moisture as environmental drivers of soil respiration, in a semiarid grassland from central Spain. Soil respiration rates were significantly lower in the dry than during the wet year, regardless of biocrust cover. Warming increased soil respiration rates, but this response was only significant in biocrust-dominated areas (> 50% biocrust cover). Warming also increased the temperature sensitivity (Q10 values) of soil respiration in biocrust-dominated areas, particularly during the wet year. The combination of warming and rainfall exclusion had similar effects in low biocrust cover areas. Our results highlight the importance of biocrusts as a modulator of soil respiration responses to both warming and rainfall exclusion, and indicate that they must be explicitly considered when evaluating soil respiration responses to climate change in drylands. PMID:25914428

  12. Spartina alterniflora invasion alters soil microbial community composition and microbial respiration following invasion chronosequence in a coastal wetland of China.

    PubMed

    Yang, Wen; Jeelani, Nasreen; Leng, Xin; Cheng, Xiaoli; An, Shuqing

    2016-01-01

    The role of exotic plants in regulating soil microbial community structure and activity following invasion chronosequence remains unclear. We investigated soil microbial community structure and microbial respiration following Spartina alterniflora invasion in a chronosequence of 6-, 10-, 17-, and 20-year-old by comparing with bare flat in a coastal wetland of China. S. alterniflora invasion significantly increased soil moisture and salinity, the concentrations of soil water-soluble organic carbon and microbial biomass carbon (MBC), the quantities of total and various types of phospholipid fatty acids (PLFAs), the fungal:bacterial PLFAs ratio and cumulative microbial respiration compared with bare flat. The highest MBC, gram-negative bacterial and saturated straight-chain PLFAs were found in 10-year-old S. alterniflora soil, while the greatest total PLFAs, bacterial and gram-positive bacterial PLFAs were found in 10- and 17-year-old S. alterniflora soils. The monounsaturated:branched PLFAs ratio declined, and cumulative microbial respiration on a per-unit-PLFAs increased following S. alterniflora invasion in the chronosequence. Our results suggest that S. alterniflora invasion significantly increased the biomass of soil various microbial groups and microbial respiration compared to bare flat soil by increasing soil available substrate, and modifying soil physiochemical properties. Soil microbial community reached the most enriched condition in the 10-year-old S. alterniflora community. PMID:27241173

  13. Spartina alterniflora invasion alters soil microbial community composition and microbial respiration following invasion chronosequence in a coastal wetland of China

    NASA Astrophysics Data System (ADS)

    Yang, Wen; Jeelani, Nasreen; Leng, Xin; Cheng, Xiaoli; An, Shuqing

    2016-05-01

    The role of exotic plants in regulating soil microbial community structure and activity following invasion chronosequence remains unclear. We investigated soil microbial community structure and microbial respiration following Spartina alterniflora invasion in a chronosequence of 6-, 10-, 17-, and 20-year-old by comparing with bare flat in a coastal wetland of China. S. alterniflora invasion significantly increased soil moisture and salinity, the concentrations of soil water-soluble organic carbon and microbial biomass carbon (MBC), the quantities of total and various types of phospholipid fatty acids (PLFAs), the fungal:bacterial PLFAs ratio and cumulative microbial respiration compared with bare flat. The highest MBC, gram-negative bacterial and saturated straight-chain PLFAs were found in 10-year-old S. alterniflora soil, while the greatest total PLFAs, bacterial and gram-positive bacterial PLFAs were found in 10- and 17-year-old S. alterniflora soils. The monounsaturated:branched PLFAs ratio declined, and cumulative microbial respiration on a per-unit-PLFAs increased following S. alterniflora invasion in the chronosequence. Our results suggest that S. alterniflora invasion significantly increased the biomass of soil various microbial groups and microbial respiration compared to bare flat soil by increasing soil available substrate, and modifying soil physiochemical properties. Soil microbial community reached the most enriched condition in the 10-year-old S. alterniflora community.

  14. Spartina alterniflora invasion alters soil microbial community composition and microbial respiration following invasion chronosequence in a coastal wetland of China

    PubMed Central

    Yang, Wen; Jeelani, Nasreen; Leng, Xin; Cheng, Xiaoli; An, Shuqing

    2016-01-01

    The role of exotic plants in regulating soil microbial community structure and activity following invasion chronosequence remains unclear. We investigated soil microbial community structure and microbial respiration following Spartina alterniflora invasion in a chronosequence of 6-, 10-, 17-, and 20-year-old by comparing with bare flat in a coastal wetland of China. S. alterniflora invasion significantly increased soil moisture and salinity, the concentrations of soil water-soluble organic carbon and microbial biomass carbon (MBC), the quantities of total and various types of phospholipid fatty acids (PLFAs), the fungal:bacterial PLFAs ratio and cumulative microbial respiration compared with bare flat. The highest MBC, gram-negative bacterial and saturated straight-chain PLFAs were found in 10-year-old S. alterniflora soil, while the greatest total PLFAs, bacterial and gram-positive bacterial PLFAs were found in 10- and 17-year-old S. alterniflora soils. The monounsaturated:branched PLFAs ratio declined, and cumulative microbial respiration on a per-unit-PLFAs increased following S. alterniflora invasion in the chronosequence. Our results suggest that S. alterniflora invasion significantly increased the biomass of soil various microbial groups and microbial respiration compared to bare flat soil by increasing soil available substrate, and modifying soil physiochemical properties. Soil microbial community reached the most enriched condition in the 10-year-old S. alterniflora community. PMID:27241173

  15. Soil moisture effects on the carbon isotopic composition of soil respiration

    EPA Science Inventory

    The carbon isotopic composition ( 13C) of recently assimilated plant carbon is known to depend on water-stress, caused either by low soil moisture or by low atmospheric humidity. Air humidity has also been shown to correlate with the 13C of soil respiration, which suggests indir...

  16. Effects of myclobutanil on soil microbial biomass, respiration, and soil nitrogen transformations.

    PubMed

    Ju, Chao; Xu, Jun; Wu, Xiaohu; Dong, Fengshou; Liu, Xingang; Zheng, Yongquan

    2016-01-01

    A 3-month-long experiment was conducted to ascertain the effects of different concentrations of myclobutanil (0.4 mg kg(-1) soil [T1]; 1.2 mg kg(-1) soil [T3]; and 4 mg kg(-1) soil [T10]) on soil microbial biomass, respiration, and soil nitrogen transformations using two typical agricultural soils (Henan fluvo-aquic soil and Shanxi cinnamon soil). Soil was sampled after 7, 15, 30, 60, and 90 days of incubation to determine myclobutanil concentration and microbial parameters: soil basal respiration (RB), microbial biomass carbon (MBC) and nitrogen (MBN), NO(-)3-N and NH(+)4-N concentrations, and gene abundance of total bacteria, N2-fixing bacteria, fungi, ammonia-oxidizing archaea (AOA), and ammonia-oxidizing bacteria (AOB). The half-lives of the different doses of myclobutanil varied from 20.3 to 69.3 d in the Henan soil and from 99 to 138.6 d in the Shanxi soil. In the Henan soil, the three treatments caused different degrees of short-term inhibition of RB and MBC, NH(+)4-N, and gene abundance of total bacteria, fungi, N2-fixing bacteria, AOA, and AOB, with the exception of a brief increase in NO(-)3-N content during the T10 treatment. The MBN (immobilized nitrogen) was not affected. In the Shanxi soil, MBC, the populations of total bacteria, fungi, and N2-fixing bacteria, and NH(+)4-N concentration were not significantly affected by myclobutanil. The RB and MBN were decreased transitorily in the T10 treatment. The NO(-)3-N concentrations and the abundance of both AOA and AOB were erratically stimulated by myclobutanil. Regardless of whether stimulation or suppression occurred, the effects of myclobutanil on the two soil types were short term. In summary, myclobutanil had no long-term negative effects on the soil microbial biomass, respiration, and soil nitrogen transformations in the two types of soil, even at 10-fold the recommended dosage. PMID:26590854

  17. Redox Fluctuations Increase the Contribution of Lignin to Soil Respiration

    NASA Astrophysics Data System (ADS)

    Hall, S. J.; Silver, W. L.; Timokhin, V.; Hammel, K.

    2014-12-01

    Lignin mineralization represents a critical flux in the terrestrial carbon (C) cycle, yet little is known about mechanisms and environmental factors controlling lignin breakdown in mineral soils. Hypoxia has long been thought to suppress lignin decomposition, yet variation in oxygen (O2) availability in surface soils accompanying moisture fluctuations could potentially stimulate this process by generating reactive oxygen species via coupled biotic and abiotic iron (Fe) redox cycling. Here, we tested the impact of redox fluctuations on lignin breakdown in humid tropical forest soils during ten-week laboratory incubations. We used synthetic lignins labeled with 13C in either of two positions (aromatic methoxyl and propyl Cβ) to provide highly sensitive and specific measures of lignin mineralization not previously employed in soils. Four-day redox fluctuations increased the percent contribution of methoxyl C to soil respiration, and cumulative methoxyl C mineralization was equivalent under static aerobic and fluctuating redox conditions despite lower total C mineralization in the latter treatment. Contributions of the highly stable Cβ to mineralization were also equivalent in static aerobic and fluctuating redox treatments during periods of O2 exposure, and nearly doubled in the fluctuating treatment after normalizing to cumulative O2 exposure. Oxygen fluctuations drove substantial net Fe reduction and oxidation, implying that reactive oxygen species generated during abiotic Fe oxidation likely contributed to the elevated contribution of lignin to C mineralization. Iron redox cycling provides a mechanism for lignin breakdown in soils that experience conditions unfavorable for canonical lignin-degrading organisms, and provides a potential mechanism for lignin depletion in soil organic matter during late-stage decomposition. Thus, close couplings between soil moisture, redox fluctuations, and lignin breakdown provide potential a link between climate variability and

  18. Permafrost collapse alters soil carbon stocks, respiration, CH4 , and N2O in upland tundra.

    PubMed

    Abbott, Benjamin W; Jones, Jeremy B

    2015-12-01

    Release of greenhouse gases from thawing permafrost is potentially the largest terrestrial feedback to climate change and one of the most likely to occur; however, estimates of its strength vary by a factor of thirty. Some of this uncertainty stems from abrupt thaw processes known as thermokarst (permafrost collapse due to ground ice melt), which alter controls on carbon and nitrogen cycling and expose organic matter from meters below the surface. Thermokarst may affect 20-50% of tundra uplands by the end of the century; however, little is known about the effect of different thermokarst morphologies on carbon and nitrogen release. We measured soil organic matter displacement, ecosystem respiration, and soil gas concentrations at 26 upland thermokarst features on the North Slope of Alaska. Features included the three most common upland thermokarst morphologies: active-layer detachment slides, thermo-erosion gullies, and retrogressive thaw slumps. We found that thermokarst morphology interacted with landscape parameters to determine both the initial displacement of organic matter and subsequent carbon and nitrogen cycling. The large proportion of ecosystem carbon exported off-site by slumps and slides resulted in decreased ecosystem respiration postfailure, while gullies removed a smaller portion of ecosystem carbon but strongly increased respiration and N2 O concentration. Elevated N2 O in gully soils persisted through most of the growing season, indicating sustained nitrification and denitrification in disturbed soils, representing a potential noncarbon permafrost climate feedback. While upland thermokarst formation did not substantially alter redox conditions within features, it redistributed organic matter into both oxic and anoxic environments. Across morphologies, residual organic matter cover, and predisturbance respiration explained 83% of the variation in respiration response. Consistent differences between upland thermokarst types may contribute to the

  19. Wet meadow ecosystems contribute the majority of overwinter soil respiration from snow-scoured alpine tundra

    NASA Astrophysics Data System (ADS)

    Knowles, John F.; Blanken, Peter D.; Williams, Mark W.

    2016-04-01

    We measured soil respiration across a soil moisture gradient ranging from dry to wet snow-scoured alpine tundra soils throughout three winters and two summers. In the absence of snow accumulation, soil moisture variability was principally determined by the combination of mesotopographical hydrological focusing and shallow subsurface permeability, which resulted in a patchwork of comingled ecosystem types along a single alpine ridge. To constrain the subsequent carbon cycling variability, we compared three measures of effective diffusivity and three methods to calculate gradient method soil respiration from four typical vegetation communities. Overwinter soil respiration was primarily restricted to wet meadow locations, and a conservative estimate of the rate of overwinter soil respiration from snow-scoured wet meadow tundra was 69-90% of the maximum carbon dioxide (CO2) respired by seasonally snow-covered soils within this same catchment. This was attributed to higher overwinter soil temperatures at wet meadow locations relative to fellfield, dry meadow, and moist meadow communities, which supported liquid water and heterotrophic respiration throughout the winter. These results were corroborated by eddy covariance-based measurements that demonstrated an average of 272 g C m-2 overwinter carbon loss during the study period. As a result, we updated a conceptual model of soil respiration versus snow cover to express the potential for soil respiration variability from snow-scoured alpine tundra.

  20. Soil respiration in four different land use systems in north central Alberta, Canada

    NASA Astrophysics Data System (ADS)

    Arevalo, Carmela B. M.; Bhatti, Jagtar S.; Chang, Scott X.; Jassal, Rachhpal S.; Sidders, Derek

    2010-03-01

    This study compares soil respiration and its heterotrophic and autotrophic components in four land use types: agriculture, 2 and 9 year old hybrid poplar plantations, grassland, and a native aspen stand in north central Alberta, Canada, over a period of two growing seasons (2006 and 2007). The differences were examined with respect to substrate quality and quantity, fine root biomass, and nutrient availability, in addition to soil temperature and soil water content. Cumulative soil C loss via soil respiration averaged over the two growing seasons was (in decreasing order) 781, 551, 523, 502, and 428 g C m-2 for native aspen stand, 9 year old hybrid poplar plantation, grassland, agriculture and 2 year old hybrid poplar plantation, respectively. We found that ˜75% of soil respiration in the native aspen stand originated from the top 7.5-10 cm litter-fibric-humus layer. Seasonal heterotrophic and autotrophic respiration among the land uses ranged from 97 to 272 and 333 to 560 g C m-2, respectively, contributing up to 35% and 83% of total soil respiration, respectively. The variability in soil respiration across different land uses was explained mainly by site differences in soil temperature (88-94%). Soil respiration followed a pronounced seasonal trend: increasing during the growing season and converging to a minimum in the fall. Soil respiration under different land uses was influenced by (1) ecosystem C stock, (2) temperature sensitivity (Q10) of organic matter present, and (3) organic matter decomposability as indicated by the natural abundance of δ13C. Heterotrophic respiration was influenced by soil temperature, while autotrophic respiration was influenced by fine root biomass and nutrient (NO3- and P) availability. These results are useful in estimating potential responses of soil respiration and its components to future land management and climate change.

  1. Diurnal variation in soil respiration under different land uses on Taihang Mountain, North China

    NASA Astrophysics Data System (ADS)

    Liu, Xiuping; Zhang, Wanjun; Zhang, Bin; Yang, Qihong; Chang, Jianguo; Hou, Ke

    2016-01-01

    The aim of this paper is to evaluate the diurnal variation in soil respiration under different land use types on Taihang Mountain, North China, and to understand its response to environmental factors (e.g., soil temperature and moisture) and forest management. Diurnal variations in soil respiration from plantations (Robinia pseudoacacia, Punica granatum, and Ziziphus jujuba), naturally regenerated forests (Vitex negundo var. heterophylla), grasslands (Bothriochloa ischaemum), and farmlands (winter wheat/summer maize) were measured using an LI-8100 automated soil CO2 flux system from May 2012 to April 2013. The results indicated that land use type had a significant effect on the diurnal variation of soil respiration. The diurnal soil respiration from farmlands was highest, followed by Ziziphus jujube, R. pseudoacacia, P. granatum, the lower soil CO2 efflux was found from B. ischaemum and V. negundo var. heterophylla. The diurnal soil respiration across different land use types was significantly affected by soil temperature and moisture, and their interaction. Precipitation-stimulated soil respiration increased more in soil with low water content and less in soil with high water content. The lower diurnal soil respiration from naturally regenerated forests suggests that naturally regenerated vegetation is the optimal vegetation type for reducing global warming.

  2. Using O2 to study the relationships between soil CO2 efflux and soil respiration

    NASA Astrophysics Data System (ADS)

    Angert, A.; Yakir, D.; Rodeghiero, M.; Preisler, Y.; Davidson, E. A.; Weiner, T.

    2015-04-01

    Soil respiration is the sum of respiration processes in the soil and is a major flux in the global carbon cycle. It is usually assumed that the CO2 efflux is equal to the soil respiration rate. Here we challenge this assumption by combining measurements of CO2 with high-precision measurements of O2. These measurements were conducted on different ecosystems and soil types and included measurements of air samples taken from the soil profile of three Mediterranean sites: a temperate forest and two alpine forests. Root-free soils from the alpine sites were also incubated in the lab. We found that the ratio between the CO2 efflux and the O2 influx (defined as apparent respiratory quotient, ARQ) was in the range of 0.14 to 1.23 and considerably deviated from the value of 0.9 ± 0.1 expected from the elemental composition of average plants and soil organic matter. At the Mediterranean sites, these deviations are explained as a result of CO2 dissolution in the soil water and transformation to bicarbonate ions in these high-pH soils, as well as by carbonate mineral dissolution and precipitation processes. Thus, a correct estimate of the short-term, chamber-based biological respiratory flux in such soils can only be made by dividing the measured soil CO2 efflux by the average (efflux-weighted) soil profile ARQ. Applying this approach to a semiarid pine forest resulted in an estimated short-term biological respiration rate that is 3.8 times higher than the chamber-measured surface CO2. The ARQ values often observed in the more acidic soils were unexpectedly low (< 0.7). These values probably result from the oxidation of reduced iron, which has been formed previously during times of high soil moisture and local anaerobic conditions inside soil aggregates. The results reported here provide direct quantitative evidence of a large temporal decoupling between soil-gas exchange fluxes and biological soil respiration.

  3. Gap filling strategies and error in estimating annual soil respiration.

    PubMed

    Gomez-Casanovas, Nuria; Anderson-Teixeira, Kristina; Zeri, Marcelo; Bernacchi, Carl J; DeLucia, Evan H

    2013-06-01

    Soil respiration (Rsoil ) is one of the largest CO2 fluxes in the global carbon (C) cycle. Estimation of annual Rsoil requires extrapolation of survey measurements or gap filling of automated records to produce a complete time series. Although many gap filling methodologies have been employed, there is no standardized procedure for producing defensible estimates of annual Rsoil . Here, we test the reliability of nine different gap filling techniques by inserting artificial gaps into 20 automated Rsoil records and comparing gap filling Rsoil estimates of each technique to measured values. We show that although the most commonly used techniques do not, on average, produce large systematic biases, gap filling accuracy may be significantly improved through application of the most reliable methods. All methods performed best at lower gap fractions and had relatively high, systematic errors for simulated survey measurements. Overall, the most accurate technique estimated Rsoil based on the soil temperature dependence of Rsoil by assuming constant temperature sensitivity and linearly interpolating reference respiration (Rsoil at 10 °C) across gaps. The linear interpolation method was the second best-performing method. In contrast, estimating Rsoil based on a single annual Rsoil - Tsoil relationship, which is currently the most commonly used technique, was among the most poorly-performing methods. Thus, our analysis demonstrates that gap filling accuracy may be improved substantially without sacrificing computational simplicity. Improved and standardized techniques for estimation of annual Rsoil will be valuable for understanding the role of Rsoil in the global C cycle. PMID:23504959

  4. Modelling in situ enzyme potential of soils: a tool to predict soil respiration from agricultural fields

    NASA Astrophysics Data System (ADS)

    Shahbaz Ali, Rana; Poll, Christian; Demyan, Scott; Nkwain Funkuin, Yvonne; Ingwersen, Joachim; Wizemann, Hans-Dieter; Kandeler, Ellen

    2014-05-01

    The fate of soil organic carbon (SOC) is one of the largest uncertainties in predicting future climate and terrestrial ecosystem functions. Extra-cellular enzymes, produced by microorganisms, perform the very first step in SOC degradation and serve as key components in global carbon cycling. Very little information is available about the seasonal variation in the temperature sensitivity of soil enzymes. Here we aim to model in situ enzyme potentials involved in the degradation of either labile or recalcitrant organic compounds to understand the temporal variability of degradation processes. To identify the similarities in seasonal patterns of soil respiration and in situ enzyme potentials, we compared the modelled in situ enzyme activities with weekly measured soil CO2 emissions. Arable soil samples from two different treatments (4 years fallow and currently vegetated plots; treatments represent range of carbon input into soil) were collected every month from April, 2012 to April, 2013, from two different study regions (Kraichgau and Swabian Alb) in Southwest Germany. The vegetation plots were under crop rotation in both study areas. We measured activities of three enzymes including β-glucosidase, xylanase and phenoloxidase at five different temperatures. We also measured soil microbial biomass in form of microbial carbon (Cmic). Land-use and area had significant effects (P < 0.001) on the microbial biomass; fallow plots having less Cmic than vegetation plots. Potential activities of β-glucosidase (P < 0.001) and xylanase (P < 0.01) were significantly higher in the vegetation plots of the Swabian Alb region than in the Kraichgau region. In both study areas, enzyme activities were higher during vegetation period and lower during winter which points to the importance of carbon input and/or temperature and soil moisture. We calculated the temperature sensitivity (Q10) of enzyme activities based on laboratory measurements of enzyme activities at a range of incubation

  5. Arbuscular mycorrhizal fungi regulate soil respiration and its response to precipitation change in a semiarid steppe.

    PubMed

    Zhang, Bingwei; Li, Shan; Chen, Shiping; Ren, Tingting; Yang, Zhiqiang; Zhao, Hanlin; Liang, Yu; Han, Xingguo

    2016-01-01

    Arbuscular mycorrhizal fungi (AMF) are critical links in plant-soil continuum and play a critical role in soil carbon cycles. Soil respiration, one of the largest carbon fluxes in global carbon cycle, is sensitive to precipitation change in semiarid ecosystems. In this study, a field experiment with fungicide application and water addition was conducted during 2010-2013 in a semiarid steppe in Inner Mongolia, China, and soil respiration was continuously measured to investigate the influences of AMF on soil respiration under different precipitation regimes. Results showed that soil respiration was promoted by water addition treatment especially during drought seasons, which induced a nonlinear response of soil respiration to precipitation change. Fungicide application suppressed AMF root colonization without impacts on soil microbes. AMF suppression treatment accelerated soil respiration with 2.7, 28.5 and 37.6 g C m(-2) across three seasons, which were mainly caused by the enhanced heterotrophic component. A steeper response of soil respiration rate to precipitation was found under fungicide application treatments, suggesting a greater dampening effect of AMF on soil carbon release as water availability increased. Our study highlighted the importance of AMF on soil carbon stabilization and sequestration in semiarid steppe ecosystems especially during wet seasons. PMID:26818214

  6. Arbuscular mycorrhizal fungi regulate soil respiration and its response to precipitation change in a semiarid steppe

    PubMed Central

    Zhang, Bingwei; Li, Shan; Chen, Shiping; Ren, Tingting; Yang, Zhiqiang; Zhao, Hanlin; Liang, Yu; Han, Xingguo

    2016-01-01

    Arbuscular mycorrhizal fungi (AMF) are critical links in plant–soil continuum and play a critical role in soil carbon cycles. Soil respiration, one of the largest carbon fluxes in global carbon cycle, is sensitive to precipitation change in semiarid ecosystems. In this study, a field experiment with fungicide application and water addition was conducted during 2010–2013 in a semiarid steppe in Inner Mongolia, China, and soil respiration was continuously measured to investigate the influences of AMF on soil respiration under different precipitation regimes. Results showed that soil respiration was promoted by water addition treatment especially during drought seasons, which induced a nonlinear response of soil respiration to precipitation change. Fungicide application suppressed AMF root colonization without impacts on soil microbes. AMF suppression treatment accelerated soil respiration with 2.7, 28.5 and 37.6 g C m−2 across three seasons, which were mainly caused by the enhanced heterotrophic component. A steeper response of soil respiration rate to precipitation was found under fungicide application treatments, suggesting a greater dampening effect of AMF on soil carbon release as water availability increased. Our study highlighted the importance of AMF on soil carbon stabilization and sequestration in semiarid steppe ecosystems especially during wet seasons. PMID:26818214

  7. Arbuscular mycorrhizal fungi regulate soil respiration and its response to precipitation change in a semiarid steppe

    NASA Astrophysics Data System (ADS)

    Zhang, Bingwei; Li, Shan; Chen, Shiping; Ren, Tingting; Yang, Zhiqiang; Zhao, Hanlin; Liang, Yu; Han, Xingguo

    2016-01-01

    Arbuscular mycorrhizal fungi (AMF) are critical links in plant-soil continuum and play a critical role in soil carbon cycles. Soil respiration, one of the largest carbon fluxes in global carbon cycle, is sensitive to precipitation change in semiarid ecosystems. In this study, a field experiment with fungicide application and water addition was conducted during 2010-2013 in a semiarid steppe in Inner Mongolia, China, and soil respiration was continuously measured to investigate the influences of AMF on soil respiration under different precipitation regimes. Results showed that soil respiration was promoted by water addition treatment especially during drought seasons, which induced a nonlinear response of soil respiration to precipitation change. Fungicide application suppressed AMF root colonization without impacts on soil microbes. AMF suppression treatment accelerated soil respiration with 2.7, 28.5 and 37.6 g C m-2 across three seasons, which were mainly caused by the enhanced heterotrophic component. A steeper response of soil respiration rate to precipitation was found under fungicide application treatments, suggesting a greater dampening effect of AMF on soil carbon release as water availability increased. Our study highlighted the importance of AMF on soil carbon stabilization and sequestration in semiarid steppe ecosystems especially during wet seasons.

  8. Effects of Experimental Drought on Soil Respiration and Radiocarbon Efflux from a Temperate Forest Soil

    NASA Astrophysics Data System (ADS)

    Borken, W.; Savage, K.; Davidson, E. A.; Trumbore, S. E.

    2002-12-01

    Soil respiration is affected by the water content of both mineral soil and organic horizons. A throughfall exclusion experiment was established at the Harvard Forest in central Massachusetts to quantify the importance of this variation. Weekly measurements of soil respiration began in the spring of 2001, with 4 manual flux chambers installed in each of 6 plots (5 x 5 m). In July 2001, sub-canopy roofs with translucent plastic panels were installed in 3 of the plots, while the other 3 control plots were left open. Temperature probes, TDR probes, and gas tubes were buried at 4 depths in each plot. DC half-bridges for measuring water content of organic material were installed in the O horizons. The roofs were removed in the autumn to allow leaf-fall and snowfall and were reinstalled in the spring of 2002. Hourly automated flux measurements, with one chamber in each of the 6 plots, were also started in the spring of 2002. Radiocarbon contents of CO2 emissions and concentrations within the soil have been measured periodically. Soil respiration rates were similar in control and treatment plots throughout June 2001, but once throughfall exclusion began in July, the fluxes declined in the exclusion plots. During the 84 days of 2001 that the roofs were in place, 168 mm of throughfall was diverted, and the cumulative soil respiration was 30% lower in exclusion plots compared to control plots (241 and 341 g C m-2, respectively). The automated chamber system revealed an increase in soil respiration in the control plots within minutes of a precipitation event. Soil respiration declined to pre-wetting values at the same time that the litter layer dried, about 48 hours after wetting. The Δ14CO2 from soil respiration ranged from 95 to 141‰ in the control plots and from 88 to 263‰ in the exclusion plots. The Δ14CO2 from root respiration and recently fixed carbon (within 1 year) should be 77‰ (the value of the atmosphere in 2001), so the observed values indicate that the

  9. Soil microbial respiration (CO2) of natural and anthropogenically-transformed ecosystems in Moscow region, Russia

    NASA Astrophysics Data System (ADS)

    Ivashchenko, Kristina; Ananyeva, Nadezhda; Rogovaya, Sofia; Vasenev, Viacheslav

    2016-04-01

    The CO2 concentration in modern atmosphere is increasing and one of the most reasons of it is land use changing. It is related not only with soil plowing, but also with growing urbanization and, thereby, forming the urban ecosystems. Such conversion of soil cover might be affected by efflux CO2 from soil into atmosphere. The soil CO2 efflux mainly supplies by soil microorganisms respiration (contribution around 70-90%) and plant roots respiration. Soil microbial respiration (MR) is determined in the field (in situ) and laboratory (in vitro) conditions. The measurement of soil MR in situ is labour-consuming, and for district, region and country areas it is difficult carried. We suggest to define the MR of the upper highest active 10 cm mineral soil layer (in vitro) followed by the accounting of area for different ecosystems in large region of Russia. Soils were sampled (autumn, 2011) in natural (forest, meadow) and anthropogenically-transformed (arable, urban) ecosystems of Sergiev-Posad, Taldom, Voskresenk, Shatura, Serpukhov and Serbryanye Prudy districts in Moscow region. In soil samples (total 156) the soil MR (24 h, 22°C, 60% WHC) were measured after preincubation procedure (7 d., 22°C, 55% WHC). The soil MR ranged from 0.13 (urban) to 5.41 μg CO2-C g-1 h-1 (meadow), the difference between these values was 42 times. Then, the soil MR values (per unit soil weight) were calculated per unit soil area (1 m2), the layer thickness of which was 0.1 m (soil volume weight was equaled 1 g cm-3). The high MR values were noted for forests soil (832-1410 g CO2-C m-2 yr-1) of studied districts, and the low MR values were for arable and urban soils (by 1.6-3.2 and 1.3-2.7 times less compared to forests, respectively). The MR rate of urban soil in Voskresenk district was comparable to that of corresponding meadows and it was even higher (in average by 2.3 times) in Serpukhov district. The soil MR rate of studied cities was higher by 20%, than in corresponding arable soils

  10. Biochar has no effect on soil respiration across Chinese agricultural soils.

    PubMed

    Liu, Xiaoyu; Zheng, Jufeng; Zhang, Dengxiao; Cheng, Kun; Zhou, Huimin; Zhang, Afeng; Li, Lianqing; Joseph, Stephen; Smith, Pete; Crowley, David; Kuzyakov, Yakov; Pan, Genxing

    2016-06-01

    Biochar addition to soil has been widely accepted as an option to enhance soil carbon sequestration by introducing recalcitrant organic matter. However, it remains unclear whether biochar will negate the net carbon accumulation by increasing carbon loss through CO2 efflux from soil (soil respiration). The objectives of this study were to address: 1) whether biochar addition increases soil respiration; and whether biochar application rate and biochar type (feedstock and pyrolyzing system) affect soil respiration. Two series of field experiments were carried out at 8 sites representing the main crop production areas in China. In experiment 1, a single type of wheat straw biochar was amended at rates of 0, 20 and 40 tha(-1) in four rice paddies and three dry croplands. In experiment 2, four types of biochar (varying in feedstock and pyrolyzing system) were amended at rates of 0 and 20 tha(-1) in a rice paddy under rice-wheat rotation. Results showed that biochar addition had no effect on CO2 efflux from soils consistently across sites, although it increased topsoil organic carbon stock by 38% on average. Meanwhile, CO2 efflux from soils amended with 40 t of biochar did not significantly higher than soils amended with 20 t of biochar. While the biochars used in Experiment 2 had different carbon pools and physico-chemical properties, they had no effect on soil CO2 efflux. The soil CO2 efflux following biochar addition could be hardly explained by the changes in soil physic-chemical properties and in soil microbial biomass. Thus, we argue that biochar will not negate the net carbon accumulation by increasing carbon loss through CO2 efflux in agricultural soils. PMID:26950640

  11. Partitioning soil respiration: examining the artifacts of the trenching method.

    NASA Astrophysics Data System (ADS)

    Savage, K. E.; Davidson, E. A.; Finzi, A.; Giasson, M. A.; Wehr, R. A.

    2014-12-01

    Soil respiration (Rs) is a combination of autotrophic (Ra) and heterotrophic respiration (Rh). Several methods have been developed to tease out the components of Rs, such as isotopic analyses, and removing Ra input through tree girdling and root exclusion experiments. Trenching involves severing the rooting system surrounding a plot to remove the Ra component within the plot. This method has some potential limitations. Reduced water uptake in trenched plots could change soil water content, which is one of the environmental controllers of Rs in many ecosystems. Eliminating root inputs could reduce heterotrophic decomposition of SOM via lack of priming. On the other hand, the severed dead roots may temporarily increase available carbon substrate for Rh. At the Harvard Forest, MA, we used the trenching method to partition Rs into its components Ra and Rh. Pre-trenched Rs was measured from spring to fall of 2012. In late fall of 2012, a trench was excavated to 1m depth around a 5x5m area, severing all roots. Plastic tarp was placed along the trench walls and then backfilled. Four automated Rs chambers were placed in the trenched plot and four in an un-trenched plot. Respiration was measured hourly for each chamber along with soil temperature and moisture from spring through fall of 2013 and 2014. Eighty root decomposition bags were placed in the organic soil horizon of the trenched (40) and un-trenched (40) plots at the time of trenching in 2012 and measured in 2013 and 2014. These data are being used to estimate the size and duration of any artifact due to root death. As expected, Rs was lower in the trenched plot (Rh only) than in the un-trenched plot (Rh + Ra) in 2013, but the reverse was unexpectedly observed during a period of low precipitation in 2014. High rates of ET combined with below-average precipitation dried the un-trenched plot to a point where Rh was inhibited, whereas less ET allowed the un-trenched plots to remain measurably wetter.

  12. Influence of Disturbance on Soil Respiration in Biologically Crusted Soil during the Dry Season

    PubMed Central

    Feng, Wei; Zhang, Yu-qing; Wu, Bin; Zha, Tian-shan; Jia, Xin; Qin, Shu-gao; Shao, Chen-xi; Liu, Jia-bin; Lai, Zong-rui; Fa, Ke-yu

    2013-01-01

    Soil respiration (Rs) is a major pathway for carbon cycling and is a complex process involving abiotic and biotic factors. Biological soil crusts (BSCs) are a key biotic component of desert ecosystems worldwide. In desert ecosystems, soils are protected from surface disturbance by BSCs, but it is unknown whether Rs is affected by disturbance of this crust layer. We measured Rs in three types of disturbed and undisturbed crusted soils (algae, lichen, and moss), as well as bare land from April to August, 2010, in Mu Us desert, northwest China. Rs was similar among undisturbed soils but increased significantly in disturbed moss and algae crusted soils. The variation of Rs in undisturbed and disturbed soil was related to soil bulk density. Disturbance also led to changes in soil organic carbon and fine particles contents, including declines of 60–70% in surface soil C and N, relative to predisturbance values. Once BSCs were disturbed, Q10 increased. Our findings indicate that a loss of BSCs cover will lead to greater soil C loss through respiration. Given these results, understanding the disturbance sensitivity impact on Rs could be helpful to modify soil management practices which promote carbon sequestration. PMID:24453845

  13. Does declining carbon-use efficiency explain thermal acclimation of soil respiration with warming?

    PubMed

    Tucker, Colin L; Bell, Jennifer; Pendall, Elise; Ogle, Kiona

    2013-01-01

    Enhanced soil respiration in response to global warming may substantially increase atmospheric CO2 concentrations above the anthropogenic contribution, depending on the mechanisms underlying the temperature sensitivity of soil respiration. Here, we compared short-term and seasonal responses of soil respiration to a shifting thermal environment and variable substrate availability via laboratory incubations. To analyze the data from incubations, we implemented a novel process-based model of soil respiration in a hierarchical Bayesian framework. Our process model combined a Michaelis-Menten-type equation of substrate availability and microbial biomass with an Arrhenius-type nonlinear temperature response function. We tested the competing hypotheses that apparent thermal acclimation of soil respiration can be explained by depletion of labile substrates in warmed soils, or that physiological acclimation reduces respiration rates. We demonstrated that short-term apparent acclimation can be induced by substrate depletion, but that decreasing microbial biomass carbon (MBC) is also important, and lower MBC at warmer temperatures is likely due to decreased carbon-use efficiency (CUE). Observed seasonal acclimation of soil respiration was associated with higher CUE and lower basal respiration for summer- vs. winter-collected soils. Whether the observed short-term decrease in CUE or the seasonal acclimation of CUE with increased temperatures dominates the response to long-term warming will have important consequences for soil organic carbon storage. PMID:23504736

  14. Effects of a clear-cut harvest on soil respiration in a jack pine - Lichen woodland

    USGS Publications Warehouse

    Striegl, R.G.; Wickland, K.P.

    1998-01-01

    Quantification of the components of ecosystem respiration is essential to understanding carbon (C) cycling of natural and disturbed landscapes. Soil respiration, which includes autotrophic and heterotrophic respiration from throughout the soil profile, is the second largest flux in the global carbon cycle. We measured soil respiration (soil CO2 emission) at an undisturbed mature jack pine (Pinus banksiana Lamb.) stand in Saskatchewan (old jack pine, OJP), and at a formerly continuous portion of the stand that was clear-cut during the previous winter (clear-cut, CC). Tree harvesting reduced soil CO2 emission from ???22.5 to ???9.1 mol CO2??m2 for the 1994 growing season. OJP was a small net sink of atmospheric CO2, while CC was a net source of CO2. Winter emissions were similar at both sites. Reduction of soil respiration was attributed to disruption of the soil surface and to the death of tree roots. Flux simulations for CC and OJP identify 40% of CO2 emission at the undisturbed OJP site as near-surface respiration, 25% as deep-soil respiration, and 35% as tree-root respiration. The near-surface component was larger than the estimated annual C input to soil, suggesting fast C turnover and no net C accumulation in these boreal uplands in 1994.

  15. Using O2 to study the relationships between soil CO2 efflux and soil respiration

    NASA Astrophysics Data System (ADS)

    Angert, A.; Yakir, D.; Rodeghiero, M.; Preisler, Y.; Davidson, E. A.; Weiner, T.

    2014-08-01

    Soil respiration, is the sum of respiration processes in the soil, and is a major flux in the global carbon cycle. It is usually assumed that the CO2 efflux is equal to the soil respiration rate. Here we challenge this assumption by combining measurements of CO2 with high-precision measurements of O2. These measurements were conducted on different ecosystems and soil types, and included measurements of air-samples taken from the soil profile of three Mediterranean sites, a temperate forest, and two alpine forests. Root-free soils from the alpine sites were also incubated at the lab. We found that the ratio between the CO2 efflux to the O2 influx (which we defined as apparent respiratory quotient, ARQ) was in the range of 0.14 to 1.23, which strongly deviates from 0.9 ± 0.1, which is the ratio expected from the elemental composition of average plants and soil organic matter. At the Mediterranean sites these deviations were explained as a result of CO2 dissolution in the soil water and transformation to bi-carbonate in these high pH soils, and by carbonates dissolution and precipitation processes. Thus, correct estimate of the short-term, chamber-based biological respiratory flux in such soils can only be made by dividing the measured CO2 efflux by the average (efflux weighted) soil profile ARQ. We demonstrated that applying this approach to a semiarid pine forest resulted in estimated short-term respiration rate 3.8 times higher than the chamber-measured surface CO2 efflux (8.8 μmol CO2 m-2 s-1 instead of 2.3 μmol CO2 m-2 s-1, at the time of measurement). The ARQ values that were often found for the more acidic soils were lower than 0.7, and hence surprising. These values might be the result of the oxidation of reduced iron, which could previously form during times of high soil moisture and local anaerobic conditions inside aggregates. Further research is needed to confirm that low ARQ found in non-calcareous soils, is the result of this process, which can cause

  16. Effects of fire and harvest on soil respiration in a mixed-conifer forest

    NASA Astrophysics Data System (ADS)

    Dore, S.; Fry, D.; Stephens, S.

    2012-12-01

    Forest ecosystems, and in particular forest soils, constitute a major reservoir of global terrestrial carbon and soil respiration is the largest carbon loss from these ecosystems. Disturbances can affect soil respiration, causing physical and chemical changes in soil characteristics, adding both, above and belowground necromass, and changing microclimatic conditions. This could signify an important and long term carbon loss, even higher than the carbon directly removed by the harvest or during fire. These losses need to be included when quantifying the net carbon balance of forests. We measured the impacts of prescribed fire and clear-cut tree harvest on soil respiration in a mixed-conifer forest in the central Sierra Nevada. The prescribed fire treatment was implemented in 2002 and again in 2009. Four areas were clear-cut harvested in 2010. In half of these units the soils were mechanically ripped to reduce soil compaction, a common practice in the Sierra Nevada industrial forest lands. Soil respiration was measured using two different techniques: the chamber method and the gradient method. Soil respiration was affected by treatments in two different ways. First, treatments changed soil temperature and soil water content, the main abiotic factors controlling soil respiration. The clear cut and the prescribed fire treatments created higher maximum soil temperature and more available soil water content, environmental conditions favorable to soil respiration. However, the loss of trees and thus fine roots, and the decrease of soil litter and organic layers, because of their combustion or removal, had a negative effect on soil respiration that was stronger than the positive effect due to more favorable post disturbance environmental conditions. Soil respiration rates remained steady 1-2 years after treatments and no increase or spikes of soil respiration were measured after treatments. Continuous measurements of CO2 concentrations at different soil depths improved our

  17. Scaling short-term effects of soil wetness on soil respiration to long-term soil carbon storage (Invited)

    NASA Astrophysics Data System (ADS)

    Davidson, E. A.

    2009-12-01

    Soil CO2 efflux (often called “soil respiration”) responds quickly to short-term variation in climatic factors, including soil wetness. Generally, the highest rates of soil respiration have been observed at intermediate water contents, with declining rates observed toward the dry and wet extremes. Drought limits diffusion of substrates and extracellular enzymes in thin water films, thereby reducing rates of microbial respiration. Excess soil water limits diffusion of oxygen, thereby reducing rates of aerobic respiration. However, these short-term responses of CO2 production and flux should not be confused with longer-term effects of climate on soil C storage. While a short-term drought (weeks to years) reduces soil respiration and may cause a transient accumulation of undecomposed soil organic matter that may be important for understanding seasonal and interannual variation in net C sequestration, long-term dry conditions (decades to centuries) result in lower plant productivity and lower C inputs to soils, and the net effect is generally lower soil C storage. Across the grasslands of the American Midwest, soil C storage is generally positively correlated with precipitation. This trend is probably due to a combination of differential responses of primary productivity, microbial decomposition, and mineral weathering across this precipitation gradient. Similar interactions of plant, microbial, and soil weathering processes are important for understanding responses to variation in temperature. Hence, short-term responses of soil CO2 fluxes to climatic variation cannot be scaled to long-term inferences of soil C storage unless the long-term responses of plant productivity and C inputs to soils are also simultaneously considered. In the very long term (millennia), weathering processes also affect the soil properties that determine stabilization of soil C stocks.

  18. Effects of assimilate supply on root and microbial components of soil respiration in a mountain grassland.

    NASA Astrophysics Data System (ADS)

    Schmitt, M.; Siegwolf, R.; Ekblad, A.; Pfahringer, N.; Bahn, M.

    2012-04-01

    Soil respiration is the main source of carbon emitted from terrestrial ecosystems. Soil CO2 originates from multiple processes, comprising respiration by plant roots, mycorrhizae and microbes in the rhizosphere, as well as respiration due to soil organic matter (SOM) decomposition. Thus, components of soil respiration have different controls and show varying responses to changing environmental conditions and to the supply of fresh assimilates from photosynthesis. For grasslands there is still little information available as to what extent root and microbial respiration respond to reduced or enhanced assimilate supply. The aim of this study was to assess effects of assimilate supply on root and microbial components of soil respiration in a temperate mountain grassland. Root and microbial components were separated and quantified by applying the Substrate Induced Respiration method (SIR) in situ using a δ13C labelled sucrose solution, and analysing δ13C of the subsequently respired CO2. Assimilate supply was modified by clipping and shading treatments, which strongly reduced photosynthetic C supply, and by applying a sucrose solution 8 days after clipping and shading. We tested the hypotheses that (1) due to a reduction of assimilate supply, soil respiration would be lower in the clipped and shaded than in the control treatment, that (2) the microbial contribution to soil respiration would be lower in the assimilate-limited than in the control treatments, and that (3) priming effects following the addition of sucrose would be stronger in shaded and mowed treatments than in control plots. Our results showed that clipping and shading reduced soil respiration significantly. Whilst the microbial contribution to soil respiration was 61% in control plots, it amounted to only 50-48% in clipped and shaded plots, respectively. Sucrose application did not affect root respiration in any of the plots, but generally stimulated microbial respiration. The measured priming effect

  19. Estimating soil carbon, nitrogen, and phosphorus mineralization from short-term CO2 respiration

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The measurement of soil carbon dioxide respiration is a means to gauge biological soil fertility. Test methods for respiration employed in the laboratory vary somewhat, and to date the equipment and labor required have somewhat limited more widespread adoption of such methodologies. A new method to...

  20. Response of soil respiration to a subambient to elevated CO2 gradient in grassland ecosystems

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Despite the importance of soil respiration responses to atmospheric CO2 concentration ([CO2]) for the global carbon cycle and climate change, the relationship between soil respiration and [CO2] has not been well developed, mainly because previous studies included few CO2 levels. We designed a unique...

  1. Soil type interacts with soil respiration in prairie exposed to a gradient CO2

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Changes in soil respiration due to rising atmospheric CO2 have large implications for land-atmosphere carbon balance and consequently the greenhouse effect. Here we report results from prairie exposed to a gradient of CO2 spanning from preindustrial (250ppm) levels to levels expected mid-century ...

  2. Quantification of soil respiration in forest ecosystems across China

    NASA Astrophysics Data System (ADS)

    Song, Xinzhang; Peng, Changhui; Zhao, Zhengyong; Zhang, Zhiting; Guo, Baohua; Wang, Weifeng; Jiang, Hong; Zhu, Qiuan

    2014-09-01

    We collected 139 estimates of the annual forest soil CO2 flux and 173 estimates of the Q10 value (the temperature sensitivity) assembled from 90 published studies across Chinese forest ecosystems. We analyzed the annual soil respiration (Rs) rates and the temperature sensitivities of seven forest ecosystems, including evergreen broadleaf forests (EBF), deciduous broadleaf forests (DBF), broadleaf and needleleaf mixed forests (BNMF), evergreen needleleaf forests (ENF), deciduous needleleaf forests (DNF), bamboo forests (BF) and shrubs (SF). The results showed that the mean annual Rs rate was 33.65 t CO2 ha-1 year-1 across Chinese forest ecosystems. Rs rates were significantly different (P < 0.001) among the seven forest types, and were significantly and positively influenced by mean annual temperature (MAT), mean annual precipitation (MAP), and actual evapotranspiration (AET); but negatively affected by latitude and elevation. The mean Q10 value of 1.28 was lower than the world average (1.4-2.0). The Q10 values derived from the soil temperature at a depth of 5 cm varied among forest ecosystems by an average of 2.46 and significantly decreased with the MAT but increased with elevation and latitude. Moreover, our results suggested that an artificial neural network (ANN) model can effectively predict Rs across Chinese forest ecosystems. This study contributes to better understanding of Rs across Chinese forest ecosystems and their possible responses to global warming.

  3. Use of the Solvita® gel system to measure soil carbon dioxide respiration as a measure of soil fertility

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The measurement of soil carbon dioxide respiration is a means to gauge biological soil fertility. Test methods for respiration employed in the laboratory vary somewhat, and to date the equipment and labor required have somewhat limited more widespread adoption of such methodologies. A new method to ...

  4. Soil respiration in the cold desert environment of the Colorado Plateau (USA): Abiotic regulators and thresholds

    USGS Publications Warehouse

    Fernandez, D.P.; Neff, J.C.; Belnap, J.; Reynolds, R.L.

    2006-01-01

    Decomposition is central to understanding ecosystem carbon exchange and nutrient-release processes. Unlike mesic ecosystems, which have been extensively studied, xeric landscapes have received little attention; as a result, abiotic soil-respiration regulatory processes are poorly understood in xeric environments. To provide a more complete and quantitative understanding about how abiotic factors influence soil respiration in xeric ecosystems, we conducted soil- respiration and decomposition-cloth measurements in the cold desert of southeast Utah. Our study evaluated when and to what extent soil texture, moisture, temperature, organic carbon, and nitrogen influence soil respiration and examined whether the inverse-texture hypothesis applies to decomposition. Within our study site, the effect of texture on moisture, as described by the inverse texture hypothesis, was evident, but its effect on decomposition was not. Our results show temperature and moisture to be the dominant abiotic controls of soil respiration. Specifically, temporal offsets in temperature and moisture conditions appear to have a strong control on soil respiration, with the highest fluxes occurring in spring when temperature and moisture were favorable. These temporal offsets resulted in decomposition rates that were controlled by soil moisture and temperature thresholds. The highest fluxes of CO2 occurred when soil temperature was between 10 and 16??C and volumetric soil moisture was greater than 10%. Decomposition-cloth results, which integrate decomposition processes across several months, support the soil-respiration results and further illustrate the seasonal patterns of high respiration rates during spring and low rates during summer and fall. Results from this study suggest that the parameters used to predict soil respiration in mesic ecosystems likely do not apply in cold-desert environments. ?? Springer 2006.

  5. Soil heterotrophic respiration responses to meteorology, soil types and cropping systems in a temperate agricultural watershed.

    NASA Astrophysics Data System (ADS)

    Buysse, Pauline; Viaud, Valérie; Fléchard, Chris

    2015-04-01

    Within the context of Climate Change, a better understanding of soil organic matter dynamics is of considerable importance in agro-ecosystems, due to their large mitigation potential. This study aims at better understanding the process of soil heterotrophic respiration at the annual scale and at the watershed scale, with these temporal and spatial scales allowing an integration of the most important drivers: cropping systems and management, topography, soil types, soil organic carbon content and meteorological conditions. Twenty-four soil CO2 flux measurement sites - comprising three PVC collars each - were spread over the Naizin-Kervidy catchment (ORE AgrHys, 4.9 km², W. France) in March 2014. These sites were selected in order to represent most of the diversity in drainage classes, soil types and cropping systems. Soil CO2 flux measurements were performed about every ten to fifteen days at each site, starting from 20 March 2014, using the dynamic closed chamber system Li-COR 8100. Soil temperature and soil moisture content down to 5 cm depth were measured simultaneously. An empirical model taking the influence of meteorological drivers (soil temperature and soil water content) on soil CO2 fluxes was applied to each site and the different responses were analyzed with regard to site characteristics (topography, soil organic carbon content, soil microbial biomass, crop type, crop management,…) in order to determine the most important driving variables of soil heterotrophic respiration. The modeling objective is then to scale the fluxes measured at all sites up to the full watershed scale.

  6. Nutrient Enrichment Mediates the Relationships of Soil Microbial Respiration with Climatic Factors in an Alpine Meadow

    PubMed Central

    Zong, Ning; Jiang, Jing; Shi, Peili; Song, Minghua; Shen, Zhenxi; Zhang, Xianzhou

    2015-01-01

    Quantifying the effects of nutrient additions on soil microbial respiration (Rm) and its contribution to soil respiration (Rs) are of great importance for accurate assessment ecosystem carbon (C) flux. Nitrogen (N) addition either alone (coded as LN and HN) or in combination with phosphorus (P) (coded as LN + P and HN + P) were manipulated in a semiarid alpine meadow on the Tibetan Plateau since 2008. Either LN or HN did not affect Rm, while LN + P enhanced Rm during peak growing periods, but HN + P did not affect Rm. Nutrient addition also significantly affected Rm/Rs, and the correlations of Rm/Rs with climatic factors varied with years. Soil water content (Sw) was the main factor controlling the variations of Rm/Rs. During the years with large rainfall variations, Rm/Rs was negatively correlated with Sw, while, in years with even rainfall, Rm/Rs was positively correlated with Sw. Meanwhile, in N + P treatments the controlling effects of climatic factors on Rm/Rs were more significant than those in CK. Our results indicate that the sensitivity of soil microbes to climatic factors is regulated by nutrient enrichment. The divergent effects of Sw on Rm/Rs suggest that precipitation distribution patterns are key factors controlling soil microbial activities and ecosystem C fluxes in semiarid alpine meadow ecosystems. PMID:26347902

  7. Plant community structure regulates responses of prairie soil respiration to decadal experimental warming.

    PubMed

    Xu, Xia; Shi, Zheng; Li, Dejun; Zhou, Xuhui; Sherry, Rebecca A; Luo, Yiqi

    2015-10-01

    Soil respiration is recognized to be influenced by temperature, moisture, and ecosystem production. However, little is known about how plant community structure regulates responses of soil respiration to climate change. Here, we used a 13-year field warming experiment to explore the mechanisms underlying plant community regulation on feedbacks of soil respiration to climate change in a tallgrass prairie in Oklahoma, USA. Infrared heaters were used to elevate temperature about 2 °C since November 1999. Annual clipping was used to mimic hay harvest. Our results showed that experimental warming significantly increased soil respiration approximately from 10% in the first 7 years (2000-2006) to 30% in the next 6 years (2007-2012). The two-stage warming stimulation of soil respiration was closely related to warming-induced increases in ecosystem production over the years. Moreover, we found that across the 13 years, warming-induced increases in soil respiration were positively affected by the proportion of aboveground net primary production (ANPP) contributed by C3 forbs. Functional composition of the plant community regulated warming-induced increases in soil respiration through the quantity and quality of organic matter inputs to soil and the amount of photosynthetic carbon (C) allocated belowground. Clipping, the interaction of clipping with warming, and warming-induced changes in soil temperature and moisture all had little effect on soil respiration over the years (all P > 0.05). Our results suggest that climate warming may drive an increase in soil respiration through altering composition of plant communities in grassland ecosystems. PMID:25846478

  8. Respirator Performance against Nanoparticles under Simulated Workplace Activities.

    PubMed

    Vo, Evanly; Zhuang, Ziqing; Horvatin, Matthew; Liu, Yuewei; He, Xinjian; Rengasamy, Samy

    2015-10-01

    Filtering facepiece respirators (FFRs) and elastomeric half-mask respirators (EHRs) are commonly used by workers for protection against potentially hazardous particles, including engineered nanoparticles. The purpose of this study was to evaluate the performance of these types of respirators against 10-400 nm particles using human subjects exposed to NaCl aerosols under simulated workplace activities. Simulated workplace protection factors (SWPFs) were measured for eight combinations of respirator models (2 N95 FFRs, 2 P100 FFRs, 2 N95 EHRs, and 2 P100 EHRs) worn by 25 healthy test subjects (13 females and 12 males) with varying face sizes. Before beginning a SWPF test for a given respirator model, each subject had to pass a quantitative fit test. Each SWPF test was performed using a protocol of six exercises for 3 min each: (i) normal breathing, (ii) deep breathing, (iii) moving head side to side, (iv) moving head up and down, (v) bending at the waist, and (vi) a simulated laboratory-vessel cleaning motion. Two scanning mobility particle sizers were used simultaneously to measure the upstream (outside the respirator) and downstream (inside the respirator) test aerosol; SWPF was then calculated as a ratio of the upstream and downstream particle concentrations. In general, geometric mean SWPF (GM-SWPF) was highest for the P100 EHRs, followed by P100 FFRs, N95 EHRs, and N95 FFRs. This trend holds true for nanoparticles (10-100 nm), larger size particles (100-400 nm), and the 'all size' range (10-400 nm). All respirators provided better or similar performance levels for 10-100 nm particles as compared to larger 100-400 nm particles. This study found that class P100 respirators provided higher SWPFs compared to class N95 respirators (P < 0.05) for both FFR and EHR types. All respirators provided expected performance (i.e. fifth percentile SWPF > 10) against all particle size ranges tested. PMID:26180261

  9. [Effect of vegetation types on soil respiration characteristics on a smaller scale].

    PubMed

    Yan, Jun-Xia; Li, Hong-Jian; Tang, Yi; Zhang, Yi-Hui

    2009-11-01

    Soil respiration was measured from April 2005 to December 2007 using a LICOR-6400-09 chamber connecting a LiCor-6400 portable photosynthesis system at 3 sites with same elevation and soil texture but different vegetation types. The results indicated that seasonal trend of soil respiration showed a distinct temporal change with the higher values in summer and autumn months and the lower values in winter and spring. Annual means (March to December) of soil respiration for 3 the sampling sites were(3.58 +/- 2.50), (3.82 +/- 2.75) and (4.42 +/- 3.38) micromol x (m2 x s)(-1) (p > 0.05), respectively. Released annual amount (March to December) of CO2 efflux from 3 sites was from 854.9 to 1 297.2 g x (m2 x a)(-1) and the amount was no difference between sites and among years. The fitted exponential equations of soil respiration and soil temperature for 3 sites were all significant with the R2 from 0.61 to 0.81, and the Q10 and R10 calculated from fitted parameters of the equations ranged from 2.60 to 4.50, and from 1.70 to 3.02 micromol x (m2 x s)(-1). The relationships between soil respiration and soil water content were not significant for all 3 sites with a maximum R2 of the regression equations only 0.12 (p > 0.05). However, when the soil temperature was above 10 degrees C, the relationships between soil respiration and soil water content was significant (p < 0.05). Four combined regression equations including soil temperature and soil water content could be used to model relationships between soil respiration and both soil temperature and soil water content together, with the R2 most above 0.7, and maximum of 0.91. PMID:20063717

  10. Different soil respiration responses to litter manipulation in three subtropical successional forests

    NASA Astrophysics Data System (ADS)

    Han, Tianfeng; Huang, Wenjuan; Liu, Juxiu; Zhou, Guoyi; Xiao, Yin

    2015-12-01

    Aboveground litter inputs have been greatly altered by human disturbances and climate change, which have important effects on soil respiration. However, the knowledge of how soil respiration responds to altered litter inputs is limited in tropical and subtropical forests. We conducted an aboveground litterfall manipulation experiment in three successional forests in the subtropics to examine the soil respiration responses to different litter inputs from January 2010 to July 2012. The soil respiration decreased by 35% in the litter exclusion treatments and increased by 77% in the doubled litter additions across all three forests. The reduction in soil respiration induced by the litter exclusion was greatest in the early successional forest, which may be related to a decrease in the soil moisture and shifts in the microbial community. The increase in soil respiration produced by the doubled litter addition was largest in the mature forest, which was most probably due to its relatively high quantity and quality of litterfall. Our results suggest that the effect of reduced litter inputs on the soil respiration lessened with forest succession but that the doubled litter inputs resulted in a stronger priming effect in the mature forest than in the other two forests.

  11. Different soil respiration responses to litter manipulation in three subtropical successional forests

    PubMed Central

    Han, Tianfeng; Huang, Wenjuan; Liu, Juxiu; Zhou, Guoyi; Xiao, Yin

    2015-01-01

    Aboveground litter inputs have been greatly altered by human disturbances and climate change, which have important effects on soil respiration. However, the knowledge of how soil respiration responds to altered litter inputs is limited in tropical and subtropical forests. We conducted an aboveground litterfall manipulation experiment in three successional forests in the subtropics to examine the soil respiration responses to different litter inputs from January 2010 to July 2012. The soil respiration decreased by 35% in the litter exclusion treatments and increased by 77% in the doubled litter additions across all three forests. The reduction in soil respiration induced by the litter exclusion was greatest in the early successional forest, which may be related to a decrease in the soil moisture and shifts in the microbial community. The increase in soil respiration produced by the doubled litter addition was largest in the mature forest, which was most probably due to its relatively high quantity and quality of litterfall. Our results suggest that the effect of reduced litter inputs on the soil respiration lessened with forest succession but that the doubled litter inputs resulted in a stronger priming effect in the mature forest than in the other two forests. PMID:26656136

  12. Different soil respiration responses to litter manipulation in three subtropical successional forests.

    PubMed

    Han, Tianfeng; Huang, Wenjuan; Liu, Juxiu; Zhou, Guoyi; Xiao, Yin

    2015-01-01

    Aboveground litter inputs have been greatly altered by human disturbances and climate change, which have important effects on soil respiration. However, the knowledge of how soil respiration responds to altered litter inputs is limited in tropical and subtropical forests. We conducted an aboveground litterfall manipulation experiment in three successional forests in the subtropics to examine the soil respiration responses to different litter inputs from January 2010 to July 2012. The soil respiration decreased by 35% in the litter exclusion treatments and increased by 77% in the doubled litter additions across all three forests. The reduction in soil respiration induced by the litter exclusion was greatest in the early successional forest, which may be related to a decrease in the soil moisture and shifts in the microbial community. The increase in soil respiration produced by the doubled litter addition was largest in the mature forest, which was most probably due to its relatively high quantity and quality of litterfall. Our results suggest that the effect of reduced litter inputs on the soil respiration lessened with forest succession but that the doubled litter inputs resulted in a stronger priming effect in the mature forest than in the other two forests. PMID:26656136

  13. Diel patterns of soil respiration in a moist subtropical forest: key drivers and future research needs

    NASA Astrophysics Data System (ADS)

    Gutiérrez del Arroyo, O.; Wood, T. E.

    2014-12-01

    Moist tropical forests have the highest soil respiration rates (Rs) of any terrestrial ecosystem and account for approximately one third of the world's soil carbon (C) pool. Small increases in the magnitude of Rs in these ecosystems can result in high rates of soil C loss, with significant consequences for global climate change. Identifying the climatic controls of Rs in moist subtropical forests will improve our ability to predict how this large C flux will respond to climate change. Our objectives were (1) to determine whether Rs varies on diel timescales, (2) whether diel Rs patterns vary seasonally, and (3) identify biophysical drivers of this temporal variation. We measured hourly Rs in a secondary, moist subtropical forest in Puerto Rico for a 3-year period using an automated soil respiration system (LI-COR 8100/8150 with six chambers). Concomitant with Rs we measured hourly variation in several climatic drivers (air/soil temperature, soil moisture, relative humidity, and photosynthetically active radiation). Soil respiration showed significant diel variation, with the magnitude, amplitude, and shape of these curves varying throughout the year. Overall, diel Rs peaked in the late afternoon and reached a minimum in the early morning. Diel amplitudes ranged from 1 to 7 μmol CO2 m-2 s-1, with larger amplitudes occurring in warmer months that also have higher rates of Rs. In warmer months Rs exhibited a strong bimodal pattern, and a narrower diel range with a single peak in cooler and drier months. Diel Rs was positively correlated with soil temperature, but this relationship was non-linear during the day and linear at night (i.e., hysteresis). The bimodal pattern of Rs may be due to a mid-day depression of photosynthesis when humidity is low and air temperature is high, thereby reducing transport of photosynthate to the roots and decreasing rhizospheric respiration. The hysteresis between Rs and temperature suggests multiple controls on Rs on diel time

  14. Soil respiration sensitivities to water and temperature in a revegetated desert

    NASA Astrophysics Data System (ADS)

    Zhang, Zhi-Shan; Dong, Xue-Jun; Xu, Bing-Xin; Chen, Yong-Le; Zhao, Yang; Gao, Yan-Hong; Hu, Yi-Gang; Huang, Lei

    2015-04-01

    Soil respiration in water-limited ecosystems is affected intricately by soil water content (SWC), temperature, and soil properties. Eight sites on sand-fixed dunes that revegetated in different years since 1950s, with several topographical positions and various biological soil crusts (BSCs) and soil properties, were selected, as well as a moving sand dune (MSD) and a reference steppe in the Tengger Desert of China. Intact soil samples of 20 cm in depth were taken and incubated randomly at 12 levels of SWC (0 to 0.4 m3 m-3) and at 9 levels of temperature (5 to 45°C) in a growth chamber; additionally, cryptogamic and microbial respirations (RM) were measured. Total soil respiration (RT, including cryptogamic, microbial, and root respiration) was measured for 2 years at the MSD and five sites of sand-fixed dunes. The relationship between RM and SWC under the optimal SWC condition (0.25 m3 m-3) is linear, as is the entire range of RT and SWC. The slope of linear function describes sensitivity of soil respiration to water (SRW) and reflects to soil water availability, which is related significantly to soil physical properties, BSCs, and soil chemical properties, in decreasing importance. Inversely, Q10 for RM is related significantly to abovementioned factors in increasing importance. However, Q10 for RT and respiration rate at 20°C are related significantly to soil texture and depth of BSCs and subsoil only. In conclusion, through affecting SRW, soil physical properties produce significant influences on soil respiration, especially for RT. This indicates that a definition of the biophysical meaning of SRW is necessary, considering the water-limited and coarse-textured soil in most desert ecosystems.

  15. Soil respiration in northern forests exposed to elevated atmospheric carbon dioxide and ozone.

    PubMed

    Pregitzer, Kurt; Loya, Wendy; Kubiske, Mark; Zak, Donald

    2006-06-01

    The aspen free-air CO2 and O3 enrichment (FACTS II-FACE) study in Rhinelander, Wisconsin, USA, is designed to understand the mechanisms by which young northern deciduous forest ecosystems respond to elevated atmospheric carbon dioxide (CO2) and elevated tropospheric ozone (O3) in a replicated, factorial, field experiment. Soil respiration is the second largest flux of carbon (C) in these ecosystems, and the objective of this study was to understand how soil respiration responded to the experimental treatments as these fast-growing stands of pure aspen and birch + aspen approached maximum leaf area. Rates of soil respiration were typically lowest in the elevated O3 treatment. Elevated CO2 significantly stimulated soil respiration (8-26%) compared to the control treatment in both community types over all three growing seasons. In years 6-7 of the experiment, the greatest rates of soil respiration occurred in the interaction treatment (CO2 + O3), and rates of soil respiration were 15-25% greater in this treatment than in the elevated CO2 treatment, depending on year and community type. Two of the treatments, elevated CO2 and elevated CO2 + O3, were fumigated with 13C-depleted CO2, and in these two treatments we used standard isotope mixing models to understand the proportions of new and old C in soil respiration. During the peak of the growing season, C fixed since the initiation of the experiment in 1998 (new C) accounted for 60-80% of total soil respiration. The isotope measurements independently confirmed that more new C was respired from the interaction treatment compared to the elevated CO2 treatment. A period of low soil moisture late in the 2003 growing season resulted in soil respiration with an isotopic signature 4-6 per thousand enriched in 13C compared to sample dates when the percentage soil moisture was higher. In 2004, an extended period of low soil moisture during August and early September, punctuated by a significant rainfall event, resulted in soil

  16. Gap filling strategies for annual estimates of soil respiration

    NASA Astrophysics Data System (ADS)

    Gomez-Casanovas, N.; Anderson-Teixeira, K. J.; Zeri, M.; Bernacchi, C. J.; DeLucia, E. H.

    2012-12-01

    Soil respiration (Rsoil) is one of the largest CO2 fluxes in the global carbon cycle. Quantifying the contribution of Rsoil to the global carbon cycle requires calculating annual fluxes from measurements that often are made sporadically. Rsoil records generally contain gaps. Filling data gaps is therefore requisite to accurately predict Rsoil. However, the reliability of various strategies for filling gaps in Rsoil records and scaling survey respiration measurements to an annual time scale has not yet been assessed. Here, we: 1) conducted a literature survey for gap filling strategies used to estimate annual Rsoil, and 2) evaluated the performance of different gap filling methods by analyzing the errors introduced when filling artificial gaps in annual Rsoil datasets for various ecosystem types. Gap filling methods evaluated included linear and cubic interpolation, monthly average, and exponential temperature-dependence models assuming a) a single temperature sensitivity (E) and reference Rsoil (Rref, Rsoil at 10°C) over the entire year, b) constant E and varying Rref, and c) varying E and Rref, and soil temperature and moisture-dependence methods. Artificial gaps were introduced to the datasets at 11 gap fractions (0-95% of existing data) and in a pattern replicating bi-monthly survey measurements (>99% "gap") and filled using each method. In addition, we analyzed how the timing of survey measurements (>99% gap) affected gap-filling performance, considering two time frames for measurement (9AM-5PM and 9AM-12PM) and two portions of the year (entire year and growing season only). Our literature survey identified a wide variety of gap filling methods that have been used in Rsoil records. The linear interpolation method along with the temperature-dependence Rsoil model assuming a single E and Rref over the entire year were the gap filling methods most widely used. All methods performed best at lower gap fractions and had relatively high, systematic errors for

  17. Effects of elevated atmospheric carbon dioxide and temperature on soil respiration in Douglas fir seedling systems

    SciTech Connect

    Lin, G.; Ehleringer, J.R.; Rygiewicz, P.T.

    1995-06-01

    We investigated the effect of CO{sub 2} enrichment and temperature increase on root respiration, litter decomposition, consumption of soil organic matter, and overall soil respiration. Douglas fir (Pseudotsuga menziesii) seedlings were being grown in the US EPA global climate change facility in Corvallis at two atmospheric CO{sub 2} concentrations and two temperatures. Soil-respired CO{sub 2} was collected for carbon and oxygen isotope analysis. Litter and new roots were also collected for carbon isotope analyses, and litter and root water were collected for oxygen isotope analyses. Isotope data were used to distinguish the sources of CO{sub 2} between root respiration, litter decomposition, and consumption of soil organic matter. Our results indicated that elevated CO{sub 2} and temperature increased soil respiration, and there was an interaction between CO{sub 2} and temperature. Elevated temperature increased only litter decomposition rate at ambient CO{sub 2}, but both root respiration and litter decomposition at elevated CO{sub 2}. Consumption of soil organic matter was a significant source for soil-respired CO{sub 2}, especially at elevated CO{sub 2}.

  18. Different tree species affect soil respiration spatial distribution in a subtropical forest of southern Taiwan

    NASA Astrophysics Data System (ADS)

    Chiang, Po-Neng; Yu, Jui-Chu; Wang, Ya-nan; Lai, Yen-Jen

    2014-05-01

    Global forests contain 69% of total carbon stored in forest soil and litter. But the carbon storage ability and release rate of warming gases of forest soil also affect global climate change. Soil carbon cycling processes are paid much attention by ecological scientists and policy makers because of the possibility of carbon being stored in soil via land use management. Soil respiration contributed large part of terrestrial carbon flux, but the relationship of soil respiration and climate change was still obscurity. Most of soil respiration researches focus on template and tropical area, little was known that in subtropical area. Afforestation is one of solutions to mitigate CO2 increase and to sequestrate CO2 in tree and soil. Therefore, the objective of this study is to clarify the relationship of tree species and soil respiration distribution in subtropical broad-leaves plantation in southern Taiwan. The research site located on southern Taiwan was sugarcane farm before 2002. The sugarcane was removed and fourteen broadleaved tree species were planted in 2002-2005. Sixteen plots (250m*250m) were set on 1 km2 area, each plot contained 4 subplots (170m2). The forest biomass (i.e. tree height, DBH) understory biomass, litter, and soil C were measured and analyzed at 2011 to 2012. Soil respiration measurement was sampled in each subplot in each month. The soil belongs to Entisol with over 60% of sandstone. The soil pH is 5.5 with low base cations because of high sand percentage. Soil carbon storage showed significantly negative relationship with soil bulk density (p<0.001) in research site. The differences of distribution of live tree C pool among 16 plots were affected by growth characteristic of tree species. Data showed that the accumulation amount of litterfall was highest in December to February and lowest in June. Different tree species planted in 16 plots, resulting in high spatial variation of litterfall amount. It also affected total amount of litterfall

  19. Temperature sensitivity of soil respiration rates enhanced by microbial community response.

    PubMed

    Karhu, Kristiina; Auffret, Marc D; Dungait, Jennifer A J; Hopkins, David W; Prosser, James I; Singh, Brajesh K; Subke, Jens-Arne; Wookey, Philip A; Agren, Göran I; Sebastià, Maria-Teresa; Gouriveau, Fabrice; Bergkvist, Göran; Meir, Patrick; Nottingham, Andrew T; Salinas, Norma; Hartley, Iain P

    2014-09-01

    Soils store about four times as much carbon as plant biomass, and soil microbial respiration releases about 60 petagrams of carbon per year to the atmosphere as carbon dioxide. Short-term experiments have shown that soil microbial respiration increases exponentially with temperature. This information has been incorporated into soil carbon and Earth-system models, which suggest that warming-induced increases in carbon dioxide release from soils represent an important positive feedback loop that could influence twenty-first-century climate change. The magnitude of this feedback remains uncertain, however, not least because the response of soil microbial communities to changing temperatures has the potential to either decrease or increase warming-induced carbon losses substantially. Here we collect soils from different ecosystems along a climate gradient from the Arctic to the Amazon and investigate how microbial community-level responses control the temperature sensitivity of soil respiration. We find that the microbial community-level response more often enhances than reduces the mid- to long-term (90 days) temperature sensitivity of respiration. Furthermore, the strongest enhancing responses were observed in soils with high carbon-to-nitrogen ratios and in soils from cold climatic regions. After 90 days, microbial community responses increased the temperature sensitivity of respiration in high-latitude soils by a factor of 1.4 compared to the instantaneous temperature response. This suggests that the substantial carbon stores in Arctic and boreal soils could be more vulnerable to climate warming than currently predicted. PMID:25186902

  20. Seasonal variations in the carbon isotope composition of soil-respired CO2 and the dominance of root/rhizsophere respiration in desert soils (Invited)

    NASA Astrophysics Data System (ADS)

    Breecker, D.; Driese, S. G.; Nordt, L. C.; Beverly, E.; Huntington, K. W.

    2013-12-01

    Quantifying the sources of CO2 produced in soils is important for closing ecosystem scale carbon (C) budgets and predicting the response of soil C pools to global change. Sourcing soil-respired CO2 is also important for accurately using paleosol carbonates as paleoenvironmental indicators. Here we present ten records of seasonal change in C isotope compositions of soil-respired CO2 (δ13Cr) and examine their implications for soil respiration. Measured concentrations and δ13C values of soil CO2 below 30 cm were used to calculate all δ13Cr values reported here. Distinct seasonal cycles occur in all records and the lowest/highest δ13Cr values occur during the winter/summer in 9 of the 10 records. The magnitude of seasonal δ13Cr fluctuations varies inversely with mean annual precipitation (MAP), increasing from 3‰ at 500 mm to 8‰ at 200 mm. Values for two Vertisols in subhumid climates plot off the trend, perhaps in part because winter ponding induces a closed system resulting in calculated winter δ13Cr values that are lower than actual and therefore overestimated seasonal δ13Cr amplitudes. The large seasonal variation in desert soil δ13Cr values has been attributed to seasonal variation in the magnitude of photosynthetic discrimination expressed in soil-respired CO2. Seasonal changes in C3 versus C4 productivity do not explain the observations as some of the largest δ13Cr variations occur in nearly monospecific C3 shrublands (creosotebush). A number of other explanations involving heterotrophic respiration, including soil temperature- and moisture- induced changes in respiration depth and substrate, are also rejected based on observed soil temperatures and average depths of respiration, which frequently exceed 50 cm in the driest soils studied. The observed decrease of seasonal amplitude with increasing precipitation is consistent with a stomatal control on desert soil δ13Cr values and may be caused by 1) MAP-driven increase in the component of

  1. Variation in Soil Respiration Across an Alpine Soil Moisture and Vegetation Community Gradient at Niwot Ridge, Colorado

    NASA Astrophysics Data System (ADS)

    Knowles, J. F.; Blanken, P.; Williams, M. W.

    2014-12-01

    The alpine tundra is a mosaic of comingled vegetation communities that vary predominantly as a function of landscape position, micro-scale topography, subsurface permeability, and resultant soil moisture availability. We characterized the spatio-temporal variability of soil respiration from 17 alpine tundra sites across an irregular soil moisture gradient within the footprint of ongoing eddy covariance measurements over the 2011 (wet year) and 2012 (dry year) growing seasons. We then used a soil moisture threshold as a proxy to separate the sites into fellfield, dry/moist meadow, and wet meadow tundra vegetation communities. Soil moisture and soil respiration were significantly correlated across all communities (p << 0.001), but increasing soil moisture invoked a bidirectional response from fellfield and dry/moist meadow communities (directly proportional) relative to wet meadow communities (inversely proportional). Soil temperature and soil respiration were not significantly correlated. Linearly interpolating between sampling dates, the cumulative soil respiration flux over the two growing seasons ranged from 595 to 3177 g CO2 m-2, and median fluxes were 1114, 1679, and 1400 g CO2 m-2 for fellfield, dry/moist meadow, and wet meadow sites, respectively. Ecosystem respiration from nighttime eddy covariance measurements was 618 g CO2 m-2 over the same period, suggesting that soil respiration fluxes from very dry fellfield tundra disproportionately influenced the eddy covariance data. Overall, cumulative soil respiration was 50% greater in the wet year (2011) relative to the dry year (2012); therefore increased precipitation has the potential to increase soil respiration from alpine tundra as a whole.

  2. Influence of Soil Tillage Systems on Soil Respiration and Production on Wheat, Maize and Soybean Crop

    NASA Astrophysics Data System (ADS)

    Moraru, P. I.; Rusu, T.

    2012-04-01

    Soil respiration leads to CO2 emissions from soil to the atmosphere, in significant amounts for the global carbon cycle. Soil capacity to produce CO2 varies depending on soil, season, intensity and quality of agrotechnical tillage, soil water, cultivated plant, fertilizer etc. The data presented in this paper were obtained on argic-stagnic Faeoziom (SRTS, 2003). These areas were was our research, presents a medium multiannual temperature of 8.20C, medium of multiannual rain drowns: 613 mm. The experimental variants chosen were: A. Conventional system (CS): V1-reversible plough (22-25 cm)+rotary grape (8-10 cm); B. Minimum tillage system (MT): V2 - paraplow (18-22 cm) + rotary grape (8-10 cm); V3 - chisel (18-22 cm) + rotary grape (8-10 cm);V4 - rotary grape (10-12 cm); C. No-Tillage systems (NT): V5 - direct sowing. The experimental design was a split-plot design with three replications. In one variant the area of a plot was 300 m2. The experimental variants were studied in the 3 years crop rotation: maize - soy-bean - autumn wheat. To soil respiration under different tillage practices, determinations were made for each crop in four vegetative stages (spring, 5-6 leaves, bean forming, harvest) using ACE Automated Soil CO2 Exchange System. Soil respiration varies throughout the year for all three crops of rotation, with a maximum in late spring (1383 to 2480 mmoli m-2s-1) and another in fall (2141 to 2350 mmoli m-2s-1). The determinations confirm the effect of soil tillage system on soil respiration, the daily average is lower at NT (315-1914 mmoli m-2s-1), followed by MT (318-2395 mmoli m-2s-1) and is higher in the CS (321-2480 mmol m-2s-1). Productions obtained at MT and NT don't have significant differences at wheat and are higher at soybean. The differences in crop yields are recorded at maize and can be a direct consequence of loosening, mineralization and intensive mobilization of soil fertility. Acknowledgments: This work was supported by CNCSIS

  3. Temperature-associated increases in the global soil respiration record

    SciTech Connect

    Bond-Lamberty, Benjamin; Thomson, Allison M.

    2010-03-25

    Soil respiration (RS), the flux of CO2 from the soil surface to the atmosphere, comprises the second-largest terrestrial carbon flux, but its dynamics are incompletely understood, and the global flux remains poorly constrained. Ecosystem warming experiments, modelling analyses, and biokinetics all suggest that RS should change with climate. This has been difficult to confirm observationally because of the high spatial variability of RS, inaccessibility of the soil medium, and inability of remote sensing instruments to measure large-scale RS fluxes. Given these constraints, is it possible to discern climate-driven changes in regional or global RS fluxes in the extant four-decade record of RS chamber measurements? Here we use a database of worldwide RS observations, matched with high-resolution historical climate data, to show a previously unknown temporal trend in the RS record after accounting for mean annual climate, leaf area, nitrogen deposition, and changes in CO2 measurement technique. Air temperature anomaly (deviation from the 1961-1990 mean) is significantly and positively correlated with changes in RS fluxes; both temperature and precipitation anomalies exert effects in specific biomes. We estimate that the current (2008) annual global RS flux is 98±12 Pg and has increased 0.1 Pg yr-1 over the last 20 years, implying a global RS temperature response (Q10) of 1.5. An increasing global RS flux does not necessarily constitute a positive feedback loop to the atmosphere; nonetheless, the available data are consistent with an acceleration of the terrestrial carbon cycle in response to global climate change.

  4. A novel approach for identifying the true temperature sensitivity from soil respiration measurements

    SciTech Connect

    Gu, Lianhong; Hanson, Paul J; Liu, Qing; Post, Wilfred M

    2008-01-01

    We propose a novel approach, called the localized ratio fitting (LRF), to estimating the true temperature sensitivity from soil respiration measurements, a task crucial to modeling terrestrial carbon cycle and climate but so far hindered by the inadequate conventional regression approach. LRF takes advantage of the different timescales of the pool dynamics Cinduced and environmental variation Cinduced changes in soil CO2 efflux. It first transforms the expression for soil respiration into a form suppressing the influence of soil carbon pool dynamics and then uses the transformed expression to infer the parameters of environmental sensitivities. LRF works best for high-frequency soil respiration measurements and thus is particularly suitable for analyzing time series produced by automated soil chambers and from soil incubation experiments. We evaluated the validity of LRF with both simulated (with a multipool soil organic carbon model driven by realistic plant litter input scenarios) and measured (with automated soil chambers) time series of soil respiration. LRF accurately retrieved the true temperature sensitivity from the simulated heterotrophic soil respiration while the conventional approach failed to do so. The simulation also revealed that LRF performed better than the conventional approach when a direct photosynthetic signal existed in the time series of soil respiration although even LRF could not completely eliminate the interference of photosynthetic contribution for estimating the true temperature sensitivity. Importantly, the simulation on the photosynthetic influence reproduced a typical seasonal pattern of apparent temperature sensitivity reported in the literature: higher sensitivity in winter (dormant season) and lower sensitivity in summer (growing season). Such pattern has been interpreted as an indication of temperature acclimation of soil respiration by previous studies. Our simulation now indicated that that interpretation may be incorrect. The

  5. Variation in winter snowpack depth and duration influences summer soil respiration in a subalpine meadow

    NASA Astrophysics Data System (ADS)

    Arnold, C. L.; Ghezzehei, T. A.; Berhe, A. A.

    2012-12-01

    Subalpine meadows in the Sierra Nevada rely on the depth and duration of the winter snowpack to supply ample water to restore the water table in the meadow during the spring snowmelt. This study examines the role that interannual variability in the winter snowpack plays in the overall rate of summer soil respiration along a hydrologic gradient in a subalpine meadow. Carbon dioxide efflux from the meadow was measured from June through September in 2011 and 2012 using soil collars and a LICOR 8100A infrared gas analyzer. Preliminary results show that soil respiration rates are influenced by the hydrologic gradient across the meadow, with drier regions peaking earlier in the summer as compared to wetter regions. We also show that high snowpack years can suppress soil respiration in the meadow until late in the summer season as compared to low snowpack years, where soil respiration peaks early in the summer.

  6. Effects of Precipitation Increase on Soil Respiration: A Three-Year Field Experiment in Subtropical Forests in China

    PubMed Central

    Deng, Qi; Hui, Dafeng; Zhang, Deqiang; Zhou, Guoyi; Liu, Juxiu; Liu, Shizhong; Chu, Guowei; Li, Jiong

    2012-01-01

    Background The aim of this study was to determine response patterns and mechanisms of soil respiration to precipitation increases in subtropical regions. Methodology/Principal Findings Field plots in three typical forests [i.e. pine forest (PF), broadleaf forest (BF), and pine and broadleaf mixed forest (MF)] in subtropical China were exposed under either Double Precipitation (DP) treatment or Ambient Precipitation (AP). Soil respiration, soil temperature, soil moisture, soil microbial biomass and fine root biomass were measured over three years. We tested whether precipitation treatments influenced the relationship of soil respiration rate (R) with soil temperature (T) and soil moisture (M) using R = (a+cM)exp(bT), where a is a parameter related to basal soil respiration; b and c are parameters related to the soil temperature and moisture sensitivities of soil respiration, respectively. We found that the DP treatment only slightly increased mean annual soil respiration in the PF (15.4%) and did not significantly change soil respiration in the MF and the BF. In the BF, the increase in soil respiration was related to the enhancements of both soil fine root biomass and microbial biomass. The DP treatment did not change model parameters, but increased soil moisture, resulting in a slight increase in soil respiration. In the MF and the BF, the DP treatment decreased soil temperature sensitivity b but increased basal soil respiration a, resulting in no significant change in soil respiration. Conclusion/Significance Our results indicate that precipitation increasing in subtropical regions in China may have limited effects on soil respiration. PMID:22844484

  7. Microbial properties explain temporal variation in soil respiration in a grassland subjected to nitrogen addition

    PubMed Central

    Li, Yue; Liu, Yinghui; Wu, Shanmei; Niu, Lei; Tian, Yuqiang

    2015-01-01

    The role of soil microbial variables in shaping the temporal variability of soil respiration has been well acknowledged but is poorly understood, particularly under elevated nitrogen (N) deposition conditions. We measured soil respiration along with soil microbial properties during the early, middle, and late growing seasons in temperate grassland plots that had been treated with N additions of 0, 2, 4, 8, 16, or 32 g N m−2 yr−1 for 10 years. Representing the averages over three observation periods, total (Rs) and heterotrophic (Rh) respiration were highest with 4 g N m−2 yr−1, but autotrophic respiration (Ra) was highest with 8 to 16 g N m−2 yr−1. Also, the responses of Rh and Ra were unsynchronized considering the periods separately. N addition had no significant impact on the temperature sensitivity (Q10) for Rs but inhibited the Q10 for Rh. Significant interactions between observation period and N level occurred in soil respiration components, and the temporal variations in soil respiration components were mostly associated with changes in microbial biomass carbon (MBC) and phospholipid fatty acids (PLFAs). Further observation on soil organic carbon and root biomass is needed to reveal the long-term effect of N deposition on soil C sequestration. PMID:26678303

  8. Microbial properties explain temporal variation in soil respiration in a grassland subjected to nitrogen addition.

    PubMed

    Li, Yue; Liu, Yinghui; Wu, Shanmei; Niu, Lei; Tian, Yuqiang

    2015-01-01

    The role of soil microbial variables in shaping the temporal variability of soil respiration has been well acknowledged but is poorly understood, particularly under elevated nitrogen (N) deposition conditions. We measured soil respiration along with soil microbial properties during the early, middle, and late growing seasons in temperate grassland plots that had been treated with N additions of 0, 2, 4, 8, 16, or 32 g N m(-2) yr(-1) for 10 years. Representing the averages over three observation periods, total (Rs) and heterotrophic (Rh) respiration were highest with 4 g N m(-2) yr(-1), but autotrophic respiration (Ra) was highest with 8 to 16 g N m(-2) yr(-1). Also, the responses of Rh and Ra were unsynchronized considering the periods separately. N addition had no significant impact on the temperature sensitivity (Q10) for Rs but inhibited the Q10 for Rh. Significant interactions between observation period and N level occurred in soil respiration components, and the temporal variations in soil respiration components were mostly associated with changes in microbial biomass carbon (MBC) and phospholipid fatty acids (PLFAs). Further observation on soil organic carbon and root biomass is needed to reveal the long-term effect of N deposition on soil C sequestration. PMID:26678303

  9. Microbial properties explain temporal variation in soil respiration in a grassland subjected to nitrogen addition

    NASA Astrophysics Data System (ADS)

    Li, Yue; Liu, Yinghui; Wu, Shanmei; Niu, Lei; Tian, Yuqiang

    2015-12-01

    The role of soil microbial variables in shaping the temporal variability of soil respiration has been well acknowledged but is poorly understood, particularly under elevated nitrogen (N) deposition conditions. We measured soil respiration along with soil microbial properties during the early, middle, and late growing seasons in temperate grassland plots that had been treated with N additions of 0, 2, 4, 8, 16, or 32 g N m-2 yr-1 for 10 years. Representing the averages over three observation periods, total (Rs) and heterotrophic (Rh) respiration were highest with 4 g N m-2 yr-1, but autotrophic respiration (Ra) was highest with 8 to 16 g N m-2 yr-1. Also, the responses of Rh and Ra were unsynchronized considering the periods separately. N addition had no significant impact on the temperature sensitivity (Q10) for Rs but inhibited the Q10 for Rh. Significant interactions between observation period and N level occurred in soil respiration components, and the temporal variations in soil respiration components were mostly associated with changes in microbial biomass carbon (MBC) and phospholipid fatty acids (PLFAs). Further observation on soil organic carbon and root biomass is needed to reveal the long-term effect of N deposition on soil C sequestration.

  10. Complex terrain alters temperature and moisture limitations of forest soil respiration across a semiarid to subalpine gradient

    NASA Astrophysics Data System (ADS)

    Berryman, E. M.; Barnard, H. R.; Adams, H. R.; Burns, M. A.; Gallo, E.; Brooks, P. D.

    2015-04-01

    Forest soil respiration is a major carbon (C) flux that is characterized by significant variability in space and time. We quantified growing season soil respiration during both a drought year and a nondrought year across a complex landscape to identify how landscape and climate interact to control soil respiration. We asked the following questions: (1) How does soil respiration vary across the catchments due to terrain-induced variability in moisture availability and temperature? (2) Does the relative importance of moisture versus temperature limitation of respiration vary across space and time? And (3) what terrain elements are important for dictating the pattern of soil respiration and its controls? Moisture superseded temperature in explaining watershed respiration patterns, with wetter yet cooler areas higher up and on north facing slopes yielding greater soil respiration than lower and south facing areas. Wetter subalpine forests had reduced moisture limitation in favor of greater seasonal temperature limitation, and the reverse was true for low-elevation semiarid forests. Coincident climate poorly predicted soil respiration in the montane transition zone; however, antecedent precipitation from the prior 10 days provided additional explanatory power. A seasonal trend in respiration remained after accounting for microclimate effects, suggesting that local climate alone may not adequately predict seasonal variability in soil respiration in montane forests. Soil respiration climate controls were more strongly related to topography during the drought year highlighting the importance of landscape complexity in ecosystem response to drought.

  11. Complex terrain alters temperature and moisture limitations of forest soil respiration across a semiarid to subalpine gradient

    USGS Publications Warehouse

    Berryman, Erin Michele; Barnard, H.R.; Adams, H.R.; Burns, M.A.; Gallo, E.; Brooks, P.D.

    2015-01-01

    Forest soil respiration is a major carbon (C) flux that is characterized by significant variability in space and time. We quantified growing season soil respiration during both a drought year and a nondrought year across a complex landscape to identify how landscape and climate interact to control soil respiration. We asked the following questions: (1) How does soil respiration vary across the catchments due to terrain-induced variability in moisture availability and temperature? (2) Does the relative importance of moisture versus temperature limitation of respiration vary across space and time? And (3) what terrain elements are important for dictating the pattern of soil respiration and its controls? Moisture superseded temperature in explaining watershed respiration patterns, with wetter yet cooler areas higher up and on north facing slopes yielding greater soil respiration than lower and south facing areas. Wetter subalpine forests had reduced moisture limitation in favor of greater seasonal temperature limitation, and the reverse was true for low-elevation semiarid forests. Coincident climate poorly predicted soil respiration in the montane transition zone; however, antecedent precipitation from the prior 10 days provided additional explanatory power. A seasonal trend in respiration remained after accounting for microclimate effects, suggesting that local climate alone may not adequately predict seasonal variability in soil respiration in montane forests. Soil respiration climate controls were more strongly related to topography during the drought year highlighting the importance of landscape complexity in ecosystem response to drought.

  12. Environmental forcing does not induce diel or synoptic variation in the carbon isotope content of forest soil respiration

    NASA Astrophysics Data System (ADS)

    Bowling, D. R.; Egan, J. E.; Hall, S. J.; Risk, D. A.

    2015-08-01

    Recent studies have examined temporal fluctuations in the amount and carbon isotope content (δ13C) of CO2 produced by the respiration of roots and soil organisms. These changes have been correlated with diel cycles of environmental forcing (e.g., sunlight and soil temperature) and with synoptic-scale atmospheric motion (e.g., rain events and pressure-induced ventilation). We used an extensive suite of measurements to examine soil respiration over 2 months in a subalpine forest in Colorado, USA (the Niwot Ridge AmeriFlux forest). Observations included automated measurements of CO2 and δ13C of CO2 in the soil efflux, the soil gas profile, and forest air. There was strong diel variability in soil efflux but no diel change in the δ13C of the soil efflux (δR) or the CO2 produced by biological activity in the soil (δJ). Following rain, soil efflux increased significantly, but δR and δJ did not change. Temporal variation in the δ13C of the soil efflux was unrelated to measured environmental variables, and we failed to find an explanation for this unexpected result. Measurements of the δ13C of the soil efflux with chambers agreed closely with independent observations of the isotopic composition of soil CO2 production derived from soil gas well measurements. Deeper in the soil profile and at the soil surface, results confirmed established theory regarding diffusive soil gas transport and isotopic fractionation. Deviation from best-fit diffusion model results at the shallower depths illuminated a pump-induced ventilation artifact that should be anticipated and avoided in future studies. There was no evidence of natural pressure-induced ventilation of the deep soil. However, higher variability in δ13C of the soil efflux relative to δ13C of production derived from soil profile measurements was likely caused by transient pressure-induced transport with small horizontal length scales.

  13. Sustained stimulation of soil respiration and CO2 release from an agricultural soil after 10 years of experimental warming

    NASA Astrophysics Data System (ADS)

    Munch, Jean Charles; Graf, Wolfgang; Reichenstein, Markus; Reth, Sascha

    2010-05-01

    A number of forest and grassland studies indicated that stimulation of the soil respiration by soil warming ceases after a couple of years (Luo et al 2001). A long-term soil warming lysimeter experiment (soil monolythes from an agricultural field, 1m2 x 2 Meter depth, temperature = ambient + 3°C; with a regionally usual crop rotation with 5 crops) was conducted in southern Germany. It results in a sustained stimulation of soil respiration after 10 years. Moreover, both warmed and control treatments exhibited a similar temperature response of soil respiration indicating that adaptation in terms of temperature sensitivity was absent. Carbon dioxide concentration measurements within the profiles are supporting these findings. The increased soil respiration occurred although vegetation productivity in the warmed treatment was not higher than in the control plots. These findings strongly contrast current soil carbon modeling concepts, where carbon pools decay according to first-order kinetics, and thus a depletion of labile soil carbon pools leads to an apparent down-regulation of microbial respiration (Knorr et al 2005). Consequently, the potential for positive carbon-climate cycle feedback may be larger than represented in current models of soil carbon turnover and in general assessments. Literatur Knorr W, Prentice I C, House J I and Holland A 2005 Long-term sensitivity of soil carbon turnover to warming Nature 433 298-301 Luo Y, Wan S, Hui D and Wallace L L 2001 Acclimatization of soil respiration to warming in a tall grass prairie Nature 413 622 - 5 Reth S. Graf W, Reichenstein M, Munch J.C. 2009 Sustained stimulation of soil respiration after 10 years of experimental warming Environmental Research Letters 4(2) 024005

  14. Salinity and nutrient contents of tidal water affects soil respiration and carbon sequestration of high and low tidal flats of Jiuduansha wetlands in different ways.

    PubMed

    Hu, Yu; Wang, Lei; Fu, Xiaohua; Yan, Jianfang; Wu, Jihua; Tsang, Yiufai; Le, Yiquan; Sun, Ying

    2016-09-15

    Soils were collected from low tidal flats and high tidal flats of Shang shoal located upstream and Xia shoal located downstream with different tidal water qualities, in the Jiuduansha wetland of the Yangtze River estuary. Soil respiration (SR) in situ and soil abiotic and microbial characteristics were studied to clarify the respective differences in the effects of tidal water salinity and nutrient levels on SR and soil carbon sequestration in low and high tidal flats. In low tidal flats, higher total nitrogen (TN) and lower salinity in the tidal water of Shang shoal resulted in higher TN and lower salinity in its soils compared with Xia shoal. These would benefit β-Proteobacteria and Anaerolineae in Shang shoal soil, which might have higher heterotrophic microbial activities and thus soil microbial respiration and SR. In low tidal flats, where soil moisture was high and the major carbon input was active organic carbon from tidal water, increasing TN was a more important factor than salinity and obviously enhanced soil microbial heterotrophic activities, soil microbial respiration and SR. While, in high tidal flats, higher salinity in Xia shoal due to higher salinity in tidal water compared with Shang shoal benefited γ-Proteobacteria which might enhance autotrophic microbial activity, and was detrimental to β-Proteobacteria in Xia shoal soil. These might have led to lower soil microbial respiration and thus SR in Xia shoal compared with Shang shoal. In high tidal flats, where soil moisture was relatively lower and the major carbon input was plant biomass that was difficult to degrade, soil salinity was the major factor restraining microbial activities, soil microbial respiration and SR. PMID:27208721

  15. Contribution of orchardgrass and white clover roots to total soil respiration

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Understanding ecosystem carbon dioxide flux requires knowledge of component fluxes including photosynthetic uptake and respiratory loss. Experimental separation of soil respiration into its heterotrophic and autotrophic components has been difficult, complicating efforts to quantify management and e...

  16. Improving Estimations of Spatial Distribution of Soil Respiration Using the Bayesian Maximum Entropy Algorithm and Soil Temperature as Auxiliary Data.

    PubMed

    Hu, Junguo; Zhou, Jian; Zhou, Guomo; Luo, Yiqi; Xu, Xiaojun; Li, Pingheng; Liang, Junyi

    2016-01-01

    Soil respiration inherently shows strong spatial variability. It is difficult to obtain an accurate characterization of soil respiration with an insufficient number of monitoring points. However, it is expensive and cumbersome to deploy many sensors. To solve this problem, we proposed employing the Bayesian Maximum Entropy (BME) algorithm, using soil temperature as auxiliary information, to study the spatial distribution of soil respiration. The BME algorithm used the soft data (auxiliary information) effectively to improve the estimation accuracy of the spatiotemporal distribution of soil respiration. Based on the functional relationship between soil temperature and soil respiration, the BME algorithm satisfactorily integrated soil temperature data into said spatial distribution. As a means of comparison, we also applied the Ordinary Kriging (OK) and Co-Kriging (Co-OK) methods. The results indicated that the root mean squared errors (RMSEs) and absolute values of bias for both Day 1 and Day 2 were the lowest for the BME method, thus demonstrating its higher estimation accuracy. Further, we compared the performance of the BME algorithm coupled with auxiliary information, namely soil temperature data, and the OK method without auxiliary information in the same study area for 9, 21, and 37 sampled points. The results showed that the RMSEs for the BME algorithm (0.972 and 1.193) were less than those for the OK method (1.146 and 1.539) when the number of sampled points was 9 and 37, respectively. This indicates that the former method using auxiliary information could reduce the required number of sampling points for studying spatial distribution of soil respiration. Thus, the BME algorithm, coupled with soil temperature data, can not only improve the accuracy of soil respiration spatial interpolation but can also reduce the number of sampling points. PMID:26807579

  17. Improving Estimations of Spatial Distribution of Soil Respiration Using the Bayesian Maximum Entropy Algorithm and Soil Temperature as Auxiliary Data

    PubMed Central

    Hu, Junguo; Zhou, Jian; Zhou, Guomo; Luo, Yiqi; Xu, Xiaojun; Li, Pingheng; Liang, Junyi

    2016-01-01

    Soil respiration inherently shows strong spatial variability. It is difficult to obtain an accurate characterization of soil respiration with an insufficient number of monitoring points. However, it is expensive and cumbersome to deploy many sensors. To solve this problem, we proposed employing the Bayesian Maximum Entropy (BME) algorithm, using soil temperature as auxiliary information, to study the spatial distribution of soil respiration. The BME algorithm used the soft data (auxiliary information) effectively to improve the estimation accuracy of the spatiotemporal distribution of soil respiration. Based on the functional relationship between soil temperature and soil respiration, the BME algorithm satisfactorily integrated soil temperature data into said spatial distribution. As a means of comparison, we also applied the Ordinary Kriging (OK) and Co-Kriging (Co-OK) methods. The results indicated that the root mean squared errors (RMSEs) and absolute values of bias for both Day 1 and Day 2 were the lowest for the BME method, thus demonstrating its higher estimation accuracy. Further, we compared the performance of the BME algorithm coupled with auxiliary information, namely soil temperature data, and the OK method without auxiliary information in the same study area for 9, 21, and 37 sampled points. The results showed that the RMSEs for the BME algorithm (0.972 and 1.193) were less than those for the OK method (1.146 and 1.539) when the number of sampled points was 9 and 37, respectively. This indicates that the former method using auxiliary information could reduce the required number of sampling points for studying spatial distribution of soil respiration. Thus, the BME algorithm, coupled with soil temperature data, can not only improve the accuracy of soil respiration spatial interpolation but can also reduce the number of sampling points. PMID:26807579

  18. Partitioning of soil respiration at the PHACE experiment: A two-method comparison

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Elevated CO2 and warming are both known to stimulate soil respiration rates, leading to concerns regarding soil-related feedback effects on climate change. We investigated soil C cycling at the Prairie Heating and CO2 Enrichment (PHACE) experiment near Cheyenne, WY, a factorial experiment combining ...

  19. Shrub encroachment alters sensitivity of soil respiration to temperature and moisture 2115

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Shrub encroachment into grasslands creates a mosaic of different soil microsites ranging from open spaces to well-developed shrub canopies, and it is unclear how this affects the spatial variability in soil respiration characteristics, such as the sensitivity to soil temperature and moisture. This i...

  20. Soil respiration dynamics under reduced rainfall in a eucalypt forest ecosystem in SE-Australia

    NASA Astrophysics Data System (ADS)

    Hinko-Najera, N.; Arndt, S. K.; Livesley, S. J.; Fest, B.

    2011-12-01

    Soil respiration is the major pathway by which CO2 is released from a forest ecosystem to the atmosphere. Hence it is a central part of the carbon balance and carbon sink strengths of forests. Soil respiration shows high spatial and temporal variability and is dependent on soil temperature and soil moisture. Its component fluxes are controlled by different carbon sources: decomposition of soil organic matter or litter (heterotrophic respiration) or allocation of assimilated carbon belowground (autotrophic respiration). As such, these processes may respond differently to environmental change. Heatwaves, fires and reduced rainfall are predicted to increase in frequency and intensity in south-eastern Australia. It is not clear how soil respiration processes will respond to this changing climate and the implications for Australia's forest carbon balance. Soil respiration has been measured under reduced rainfall treatments and control plots using closed-dynamic manual chambers linked to a Cavity Ring-Down Spectrometer in a temperate dry eucalypt forest. In addition soil greenhouse gas dynamics are monitored using automated chambers linked to a Fourier Transform Infra Red spectrometer. Forest net carbon exchange is determined using eddy-covariance measurements as part of a long-term ecosystem research project. This presentation focuses on data collected using manual chambers where soil respiration and its component heterotrophic (root exclusion), autotrophic (indirectly) and litter decomposition (indirectly) fluxes have been measured for 1.5 years with regards to their 1) spatial variability, 2) environmental drivers and 3) response to reduced rainfall (40%). First results show total soil respiration (TSR) decreased on average by 16% under reduced rainfall treatment and varied with season according to soil temperature with lowest rates during winter/autumn and highest rates during spring/summer. The contribution of heterotrophic respiration (RH) was on average 28% and

  1. The diel patterns of soil respiration in four arid California ecosystems: fluxes, sources and hypotheses

    NASA Astrophysics Data System (ADS)

    Carbone, M.; Trumbore, S.; Winston, G.; Serio, D.

    2007-12-01

    Automated measurements provide the high-resolution information that enables us to analyze potential causes for diel variability in soil respiration. These diel patterns are the complex result of biological and physical processes that determine the production and diffusion of CO2 through the soil. We examined the diel patterns of soil respiration from four arid California ecosystems: (1) a pinon-juniper woodland in at the Burns Pinon Ridge Reserve near Joshua Tree National Park, (2) a cold desert shrub community and (3) a perennial grassland near the city of Bishop in the Owens Valley, and (4) a mixed oak-pine forest at the James Reserve in the San Jacinto Mountains. In addition to automated chamber and environmental measurements at these sites, we used isotopic (14C) partitioning techniques to separate the plant and microbial sources contributing to soil respiration at certain time points. Here we present the diel cycles of soil respiration and environmental variables, the source partitioning results, and hypotheses about what processes determine these diel patterns that both span, and are specific to the studied ecosystems. In these systems dominated by Mediterranean or desert climates, we observed that factors like relative humidity can dominate the diel variations in soil respiration for sites with very dry surface litter. At other sites and times of year, diel variation in soil respiration reflects photosynthetic and VPD influence on root respiration. The combination of automated chamber measurements with isotopes provides information useful for separating the plant and heterotrophic control on diel and seasonal soil respiration fluxes.

  2. Fractional contributions by autotrophic and heterotrophic respiration to soil-surface CO2 efflux in Boreal forests.

    PubMed

    Högberg, Peter; Nordgren, Anders; Högberg, Mona N; Ottosson-Löfvenius, Mikaell; Bhupinderpal-Singh; Olsson, Per; Linder, Sune

    2005-01-01

    Soil-surface CO2 efflux ('soil respiration') accounts for roughly two-thirds of forest ecosystem respiration, and can be divided into heterotrophic and autotrophic components. Conventionally, the latter is defined as respiration by plant roots. In Boreal forests, however, fine roots of trees are invariably covered by ectomycorrhizal fungi, which by definition are heterotrophs, but like the roots, receive sugars derived from photosynthesis. There is also a significant leaching of labile carbon compounds from the ectomycorrhizal roots. It is, therefore, more meaningful in the context of carbon balance studies to include mycorrhizal fungi and other mycorrhizosphere organisms, dependent on the direct flux of labile carbon from photosynthesis, in the autotrophic component. Hence, heterotrophic activity becomes reserved for the decomposition of more complex organic molecules in litter and other forms of soil organic matter. In reality, the complex situation is perhaps best described as a continuum from strict autotrophy to strict heterotrophy. As a result of this, and associated methodological problems, estimates of the contribution of autotrophic respiration to total soil respiration have been highly variable. Based on recent stand-scale tree girdling experiments we have estimated that autotrophic respiration in boreal forest accounts for up to 50-65% of soil respiration during the snow-free part of the year. Girdling experiments and studies of the delta(13)C of the soil CO2 efflux show that there is a lag of a few days between the carbon uptake by photosynthesis and the release by autotrophic soil respiration of the assimilated carbon. In contrast, estimates of 'bomb 14C' and other approaches have suggested that it takes years to decades between carbon uptake via photosynthesis and the bulk of soil heterotrophic activity. Temperature is normally used as a driver in models of soil processes and it is often assumed that autotrophic soil activity is more sensitive to

  3. Soil respiration under climate change: prolonged summer drought offsets soil warming effects

    PubMed Central

    Schindlbacher, Andreas; Wunderlich, Steve; Borken, Werner; Kitzler, Barbara; Zechmeister-Boltenstern, Sophie; Jandl, Robert

    2012-01-01

    Climate change may considerably impact the carbon (C) dynamics and C stocks of forest soils. To assess the combined effects of warming and reduced precipitation on soil CO2 efflux, we conducted a two-way factorial manipulation experiment (4 °C soil warming + throughfall exclusion) in a temperate spruce forest from 2008 until 2010. Soil was warmed by heating cables throughout the growing seasons. Soil drought was simulated by throughfall exclusions with three 100 m2 roofs during 25 days in July/August 2008 and 2009. Soil warming permanently increased the CO2 efflux from soil, whereas throughfall exclusion led to a sharp decrease in soil CO2 efflux (45% and 50% reduction during roof installation in 2008 and 2009, respectively). In 2008, CO2 efflux did not recover after natural rewetting and remained lowered until autumn. In 2009, CO2 efflux recovered shortly after rewetting, but relapsed again for several weeks. Drought offset the increase in soil CO2 efflux by warming in 2008 (growing season CO2 efflux in t C ha−1: control: 7.1 ± 1.0; warmed: 9.5 ± 1.7; warmed + roof: 7.4 ± 0.3; roof: 5.9 ± 0.4) and in 2009 (control: 7.6 ± 0.8; warmed + roof: 8.3 ± 1.0). Throughfall exclusion mainly affected the organic layer and the top 5 cm of the mineral soil. Radiocarbon data suggest that heterotrophic and autotrophic respiration were affected to the same extent by soil warming and drying. Microbial biomass in the mineral soil (0–5 cm) was not affected by the treatments. Our results suggest that warming causes significant C losses from the soil as long as precipitation patterns remain steady at our site. If summer droughts become more severe in the future, warming induced C losses will likely be offset by reduced soil CO2 efflux during and after summer drought.

  4. Updating soil CO2 emission experiments to assess climate change effects and extracellular soil respiration

    NASA Astrophysics Data System (ADS)

    Vidal Vazquez, Eva; Paz Ferreiro, Jorge

    2014-05-01

    Experimental work is an essential component in training future soil scientists. Soil CO2 emission is a key issue because of the potential impacts of this process on the greenhouse effect. The amount of organic carbon stored in soils worldwide is about 1600 gigatons (Gt) compared to 750 Gt in the atmosphere mostly in the form of CO2. Thus, if soil respiration increased slightly so that just 10% of the soil carbon pool was converted to CO2, atmospheric CO2 concentrations in the atmosphere could increase by one-fifth. General circulation model predictions indicate atmosphere warming between 2 and 5°C (IPCC 2007) and precipitation changes ranging from about -15 to +30%. Traditionally, release of CO2 was thought to occur only in an intracellular environment; however, recently CO2 emissions have been in irradiated soil, in the absence of microorganisms (Maire et al., 2013). Moreover, soil plays a role in the stabilization of respiration enzymes promoting CO2 release after microorganism death. Here, we propose to improve CO2 emission experiments commonly used in soil biology to investigate: 1) effects of climatic factors on soil CO2 emissions, and 2) rates of extracellular respiration in soils and how these rates are affected by environmental factors. Experiment designed to assess the effect of climate change can be conducted either in field conditions under different ecosystems (forest, grassland, cropland) or in a greenhouse using simple soil chambers. The interactions of climate change in CO2 emissions are investigated using climate-manipulation experiment that can be adapted to field or greenhouse conditions (e.g. Mc Daniel et al., 2013). The experimental design includes a control plot (without soil temperature and rain manipulation) a warming treatment as well as wetting and/or drying treatments. Plots are warmed to the target temperature by procedures such as infrared heaters (field) or radiant cable (greenhouse). To analyze extracellular respiration, rates of CO2

  5. [Respiration from density fractions of two cultivated soils and its temperature sensitivity].

    PubMed

    Cai, Jin-yun; Sun, Wen-juan; Ding, Fan; Hu, Xun-yu; Chen, Yue; Huang, Yao

    2015-09-01

    To investigate respiration from density fractions of cultivated soils and its temperature sensitivity, laboratory incubations of upland and paddy soils were carried out for a period of 63 days at four temperature levels of 5, 15, 25 and 35 °C. The upland and paddy soil samples were taken from Pingyi of Shandong Province and Taojiang of Hunan Province, respectively. CO2 efflux from light fraction (LF), heavy fraction (HF) and bulk soil (BS) was measured during the incubation. The results indicated that bulk soil respiration was significantly higher than either light or heavy fraction respiration regardless of soil type. Respiration from HF was higher than that from LF in the upland soil. In the temperature range from 5 to 25 °C, light and heavy fraction respiration in the paddy soil did not show significant difference, while the HF exhibited higher respiration than the LF at 35 °C. Over the 63-day incubation with various temperatures, cumulative respiration from the LF, the HF and the BS accounted for 0.3%-2.8%, 0.4%-3.7% and 0.6%-7.0% of the original LF, HF and BS carbon in the upland soil, and 0.4%-3.0%, 0.3%-3.8% and 0.7%-5.3% of their original carbon in the paddy soil. The temperature sensitivity of the CO2 efflux from the LF, HF and BS, which was expressed as Q10 value, declined as the incubation proceeded. The Q10 values for the HF were generally higher than the values for the LF in the paddy soil, while the difference of Q10 values between the HF and the LF was divergent in the upland soil. In the temperature range from 5 to 25 °C, the Q10 values for BS respiration were higher in the upland soil than in the paddy soil, but it was opposite in the temperature range from 25 to 35 °C. Our results using the site-specific soils suggested that the decomposition of organic carbon in the upland soil was faster and could be more sensitive to temperature change than in the paddy soil. PMID:26785546

  6. Soil respiration responses to variation in temperature and moisture availability under woody plants and grasses

    NASA Astrophysics Data System (ADS)

    Pravalprukskul, P.; Pavao-Zuckerman, M.; Barron-Gafford, G. A.

    2011-12-01

    Woody plant encroachment into grasslands, such as in the southwestern US, is thought to have altered regional carbon fluxes due to the differences in structure and function between grasses and woody plants. It is unknown how climate change predictions for such areas, particularly warmer temperatures and fewer but larger precipitation events, might further acerbate our ability to estimate flux dynamics. Soil respiration, a key flux affecting ecosystem carbon balance, has been increasingly studied, but the exact effects of temperature and precipitation changes on flux rates have not been fully determined, particularly their interactive effects. The goal of this study was to compare soil respiration responses to different temperatures in soils under native southwestern mesquites and grasses undergoing a precipitation pulse, whilst removing other confounding factors, such as soil history, through the controlled environments within Biosphere 2. Mesquites and grasses were transplanted into ground basalt within two environments maintained at a 4°C temperature difference, the projected temperature increase from climate change. Post-transplant soil samples were incubated between 10 and 40°C to determine the temperature sensitivities of soils from each microhabitat within a month of this transplant. A single-peak, best-fit model for grass soils suggested a weak temperature sensitivity, while mesquite soils showed little to no sensitivity. Additionally, all plants underwent a drought treatment prior to the precipitation event, and soil respiration rates were tracked over several days using the collar technique. This portion of the study allowed for an estimation of the sensitivity of soil respiration to precipitation pulses under a variety of antecedent moisture conditions. Initial results illustrate that soils under mesquites tend to respire significantly more than soil under grasses or in bare soils over the course of a precipitation event. Together, these results suggest

  7. Vegetation Types Alter Soil Respiration and Its Temperature Sensitivity at the Field Scale in an Estuary Wetland

    PubMed Central

    Han, Guangxuan; Xing, Qinghui; Luo, Yiqi; Rafique, Rashad; Yu, Junbao; Mikle, Nate

    2014-01-01

    Vegetation type plays an important role in regulating the temporal and spatial variation of soil respiration. Therefore, vegetation patchiness may cause high uncertainties in the estimates of soil respiration for scaling field measurements to ecosystem level. Few studies provide insights regarding the influence of vegetation types on soil respiration and its temperature sensitivity in an estuary wetland. In order to enhance the understanding of this issue, we focused on the growing season and investigated how the soil respiration and its temperature sensitivity are affected by the different vegetation (Phragmites australis, Suaeda salsa and bare soil) in the Yellow River Estuary. During the growing season, there were significant linear relationships between soil respiration rates and shoot and root biomass, respectively. On the diurnal timescale, daytime soil respiration was more dependent on net photosynthesis. A positive correlation between soil respiration and net photosynthesis at the Phragmites australis site was found. There were exponential correlations between soil respiration and soil temperature, and the fitted Q10 values varied among different vegetation types (1.81, 2.15 and 3.43 for Phragmites australis, Suaeda salsa and bare soil sites, respectively). During the growing season, the mean soil respiration was consistently higher at the Phragmites australis site (1.11 µmol CO2 m−2 s−1), followed by the Suaeda salsa site (0.77 µmol CO2 m−2 s−1) and the bare soil site (0.41 µmol CO2 m−2 s−1). The mean monthly soil respiration was positively correlated with shoot and root biomass, total C, and total N among the three vegetation patches. Our results suggest that vegetation patchiness at a field scale might have a large impact on ecosystem-scale soil respiration. Therefore, it is necessary to consider the differences in vegetation types when using models to evaluate soil respiration in an estuary wetland. PMID:24608636

  8. Effects of soil moisture on the temperature sensitivity of soil heterotrophic respiration: a laboratory incubation study.

    PubMed

    Zhou, Weiping; Hui, Dafeng; Shen, Weijun

    2014-01-01

    The temperature sensitivity (Q10) of soil heterotrophic respiration (Rh) is an important ecological model parameter and may vary with temperature and moisture. While Q10 generally decreases with increasing temperature, the moisture effects on Q10 have been controversial. To address this, we conducted a 90-day laboratory incubation experiment using a subtropical forest soil with a full factorial combination of five moisture levels (20%, 40%, 60%, 80%, and 100% water holding capacity--WHC) and five temperature levels (10, 17, 24, 31, and 38°C). Under each moisture treatment, Rh was measured several times for each temperature treatment to derive Q10 based on the exponential relationships between Rh and temperature. Microbial biomass carbon (MBC), microbial community structure and soil nutrients were also measured several times to detect their potential contributions to the moisture-induced Q10 variation. We found that Q10 was significantly lower at lower moisture levels (60%, 40% and 20% WHC) than at higher moisture level (80% WHC) during the early stage of the incubation, but became significantly higher at 20%WHC than at 60% WHC and not significantly different from the other three moisture levels during the late stage of incubation. In contrast, soil Rh had the highest value at 60% WHC and the lowest at 20% WHC throughout the whole incubation period. Variations of Q10 were significantly associated with MBC during the early stages of incubation, but with the fungi-to-bacteria ratio during the later stages, suggesting that changes in microbial biomass and community structure are related to the moisture-induced Q10 changes. This study implies that global warming's impacts on soil CO2 emission may depend upon soil moisture conditions. With the same temperature rise, wetter soils may emit more CO2 into the atmosphere via heterotrophic respiration. PMID:24647610

  9. Partitioning sources of soil-respired CO2 and their seasonal variation using a unique radiocarbon tracer

    SciTech Connect

    Cisneros-Dozal, Luz Maria; Trumbore, Susan E.; Hanson, Paul J

    2006-01-01

    Soil respiration is derived from heterotrophic (decomposition of soil organic matter) and autotrophic (root/rhizosphere respiration) sources, but there is considerable uncertainty about what factors control variations in their relative contributions in space and time. We took advantage of a unique whole-ecosystem radiocarbon label in a temperate forest to partition soil respiration into three sources: (1) recently photosynthesized carbon (C), which dominates root and rhizosphere respiration; (2) leaf litter decomposition and (3) decomposition of root litter and soil organic matter 41-2 years old. Heterotrophic sources and specifically leaf litter decomposition were large contributors to total soil respiration during the growing season. Relative contributions from leaf litter decomposition ranged from a low of 1 3% of total soil respiration (63 mgCm 2 h 1) when leaf litter was extremely dry, to a high of 42 16% (96 38mgCm 2 h 1). Total soil respiration fluxes varied with the strength of the leaf litter decomposition source, indicating that moisture-dependent changes in litter decomposition drive variability in total soil respiration fluxes. In the surface mineral soil layer, decomposition of C fixed in the original labeling event (3-5 years earlier) dominated the isotopic signature of heterotrophic respiration. Root/rhizosphere respiration accounted for 16 10% to 64 22% of total soil respiration, with highest relative contributions coinciding with low overall soil respiration fluxes. In contrast to leaf litter decomposition, root respiration fluxes did not exhibit marked temporal variation ranging from 34 14 to 40 16mgCm 2 h 1 at different times in the growing season with a single exception (88 35 mgCm 2 h 1). Radiocarbon signatures of root respired CO2 changed markedly between early and late spring (March vs. May), suggesting a switch from stored nonstructural carbohydrate sources to more recent photosynthetic products.

  10. Effects of elevated atmospheric carbon dioxide and temperature on soil respiration in Douglas fir seedling systems

    SciTech Connect

    Lin, C.; Ehleringer, J.R.; Rygiewicz, P.T.

    1995-09-01

    We investigated the effect of CO{sub 2} enrichment and temperature increase on root respiration, litter decomposition, consumption of soil organic matter, and overall soil respiration. Douglas fir (Pseudotsuga menziesii) seedlings were being grown in the US EPA global climate change facility in Corvallis at two atmospheric CO{sub 2} concentrations and two temperatures. Soil-respired CO{sub 2} was collected for carbon and oxygen isotope analysis. Litter and new roots were also collected from carbon isotope analyses, and litter and root water were collected for oxygen isotope analyses. Isotope data were used to distinguish the sources of CO{sub 2} between root respiration, and there was an interaction between CO{sub 2} and temperature. Elevated temperature increased only litter decomposition rate at ambient CO{sub 2}, but both root respiration and litter decomposition at elevated CO{sub 2}. Consumption of soil organic matter was a significant source for soil-respired CO{sub 2} especially at elevated CO{sub 2}.

  11. The impact of a large industrial city on the soil respiration in forest ecosystems

    NASA Astrophysics Data System (ADS)

    Smorkalov, I. A.; Vorobeichik, E. L.

    2015-01-01

    The rate of soil respiration was measured in situ under native pine stands in a large industrial city (Yekaterinburg, Russia) and beyond it. The compared sites differed significantly in the two factors affecting soil respiration, i.e., in the degree of urbanization (including air pollution, changes in the microclimate, fragmentation of the biotopes, the appearance of introduced species, etc.) and in the character of recreation loads (primarily, trampling loads). The difference between soil respiration rates in the city and in the suburbs was significant; it reached its maximum in the summer, when the soil respiration in the city was 1.9-3.5 times lower than that in the suburbs. However, this difference was virtually absent in the spring and fall seasons. The impact of recreation loads on the soil respiration was relatively low; moreover, it could have both positive and negative signs, i.e., lead to the increase or decrease in the soil respirarion rate. The particular mechanisms explaining the influence of the considered factors on the rate of the CO2 emission from the soils are discussed.

  12. Effects of Spartina alterniflora invasion on soil respiration in the Yangtze River estuary, China.

    PubMed

    Bu, Naishun; Qu, Junfeng; Li, Zhaolei; Li, Gang; Zhao, Hua; Zhao, Bin; Li, Bo; Chen, Jiakuan; Fang, Changming

    2015-01-01

    Many studies have found that plant invasion can enhance soil organic carbon (SOC) pools, by increasing net primary production (NPP) and/or decreased soil respiration. While most studies have focused on C input, little attention has been paid to plant invasion effects on soil respiration, especially in wetland ecosystems. Our study examined the effects of Spartina alterniflora invasion on soil respiration and C dynamics in the Yangtze River estuary. The estuary was originally occupied by two native plant species: Phragmites australis in the high tide zone and Scirpus mariqueter in the low tide zone. Mean soil respiration rates were 185.8 and 142.3 mg CO2 m(-2) h(-1) in S. alterniflora and P. australis stands in the high tide zone, and 159.7 and 112.0 mg CO2 m(-2) h(-1) in S. alterniflora and S. mariqueter stands in the low tide zone, respectively. Aboveground NPP (ANPP), SOC, and microbial biomass were also significantly higher in the S. alterniflora stands than in the two native plant stands. S. alterniflora invasion did not significantly change soil inorganic carbon or pH. Our results indicated that enhanced ANPP by S. alterniflora exceeded invasion-induced C loss through soil respiration. This suggests that S. alterniflora invasion into the Yangtze River estuary could strengthen the net C sink of wetlands in the context of global climate change. PMID:25799512

  13. Effects of Spartina alterniflora Invasion on Soil Respiration in the Yangtze River Estuary, China

    PubMed Central

    Bu, Naishun; Qu, Junfeng; Li, Zhaolei; Li, Gang; Zhao, Hua; Zhao, Bin; Li, Bo; Chen, Jiakuan; Fang, Changming

    2015-01-01

    Many studies have found that plant invasion can enhance soil organic carbon (SOC) pools, by increasing net primary production (NPP) and/or decreased soil respiration. While most studies have focused on C input, little attention has been paid to plant invasion effects on soil respiration, especially in wetland ecosystems. Our study examined the effects of Spartina alterniflora invasion on soil respiration and C dynamics in the Yangtze River estuary. The estuary was originally occupied by two native plant species: Phragmites australis in the high tide zone and Scirpus mariqueter in the low tide zone. Mean soil respiration rates were 185.8 and 142.3 mg CO2 m−2 h−1 in S. alterniflora and P. australis stands in the high tide zone, and 159.7 and 112.0 mg CO2 m−2 h−1 in S. alterniflora and S. mariqueter stands in the low tide zone, respectively. Aboveground NPP (ANPP), SOC, and microbial biomass were also significantly higher in the S. alterniflora stands than in the two native plant stands. S. alterniflora invasion did not significantly change soil inorganic carbon or pH. Our results indicated that enhanced ANPP by S. alterniflora exceeded invasion-induced C loss through soil respiration. This suggests that S. alterniflora invasion into the Yangtze River estuary could strengthen the net C sink of wetlands in the context of global climate change. PMID:25799512

  14. The responses of soil respiration to nitrogen addition in a temperate grassland in northern China.

    PubMed

    Luo, Qinpu; Gong, Jirui; Zhai, Zhanwei; Pan, Yan; Liu, Min; Xu, Sha; Wang, Yihui; Yang, Lili; Baoyin, Taoge-Tao

    2016-11-01

    Anthropogenic activities have increased nitrogen (N) inputs to grassland ecosystems. Knowledge of the impact of soil N availability on soil respiration (RS) is critical to understand soil carbon balances and their responses to global climate change. A 2-year field experiment was conducted to evaluate the response of RS to soil mineral N in a temperate grassland in northern China. RS, abiotic and biotic factors, and N mineralization were measured in the grassland, at rates of N addition ranging from 0 to 25gNm(-2)yr(-1). Annual and dormant-season RS ranged from 241.34 to 283.64g C m(-2) and from 61.34 to 83.84g C m(-2) respectively. High N application significantly increased RS, possibly due to increased root biomass and increased microbial biomass. High N treatment significantly increased soil NO3-N and inorganic N content compared with the control. The ratio of NO3-N to NH4-N and the N mineralization rate were significantly positively correlated with RS, but NH4-N was not correlated or negatively correlated with RS during the growing season. The temperature sensitivity of RS (Q10) was not significantly affected by N levels, and ranged from 1.90 to 2.20, but decreased marginally significantly at high N. RS outside the growing season is an important component of annual RS, accounting for 25.0 to 29.6% of the total. High N application indirectly stimulated RS by increasing soil NO3-N and net nitrification, thereby eliminating soil N limitations, promoting ecosystem productivity, and increasing soil CO2 efflux. Our results show the importance of distinguishing between NO3-N and NH4-N, as their impact on soil CO2 efflux differed. PMID:27396319

  15. Characterization of soil respiration rates along a temporal restoration gradient in a degenerated wetland of the arid region in NW-China

    NASA Astrophysics Data System (ADS)

    Wu, Z.; Behrendt, T.; Mamtimin, B.; Meixner, F.

    2012-04-01

    restoration period, (b)soil physical-chemical properties show a de-salinization trend, (c)annual course of soil respiration at the end-stage restoration site (only one year of restoration) is characterized by a single maximum occurring in autumn. The soil respiration at the medium- and long-term restoration sites is characterized by two maxima, one in spring and one in autumn, where the value in springtime was higher than that in autumn, (d)soil respiration rates gradually increase with the vegetation recovery, (e)near-surface air temperature (at 0.1 m above the soil) was closely correlated with soil respiration rates at the sites of different restoration periods. We found, that (1) the restoration project was useful for the degenerated Jinhe wetland, resulting in the enhancement of soil activity and soil quality, (2) near-surface air temperature seems to be the most important factor which impacts the soil respiration (and not soil moisture), (3) the degenerated wetland will return to its initial status, if sufficient water could be supplied, (4) along with the increase of soil respiration rates the eco-environment of the degenerated wetland obviously has been improved. However, with more CO2 the wetland will also emit more CH4 unfortunately from the perspective of climate change.

  16. Microbial respiration and organic carbon indicate nutrient cycling recovery in reclaimed soils

    SciTech Connect

    Ingram, L.J.; Schuman, G.E.; Stahl, P.D.; Spackman, L.K.

    2005-12-01

    Soil quality and the ability of soil to sustain nutrient cycling in drastically disturbed ecosystems will influence the establishment and maintenance of a permanent and stable plant community. We undertook research to evaluate a recently developed method to assess soil quality and nutrient cycling potential in a series of reclaimed soils. The method involves correlating the 3-d flush of microbial respiration after a soil is rewetted against a range of soil biological parameters. Soils were sampled from a number of reclaimed coal mines, a reclaimed uranium mine, and native, undisturbed prairie. All sites were located in semiarid Wyoming.

  17. Influence of Soil Moisture on Litter Respiration in the Semiarid Loess Plateau

    PubMed Central

    Zhang, Yanjun; Guo, Shengli; Liu, Qingfang; Jiang, Jishao

    2014-01-01

    Understanding the response mechanisms of litter respiration to soil moisture in water-limited semi-arid regions is of vital importance to better understanding the interplay between ecological processes and the local carbon cycle. In situ soil respiration was monitored during 2010–2012 under various conditions (normal litter, no litter, and double litter treatments) in a 30-year-old artificial black locust plantation (Robinia pseudoacacia L.) on the Loess Plateau. Litter respiration with normal and double litter treatments exhibited similar seasonal variation, with the maximum value obtained in summer (0.57 and 1.51 μmol m−2 s−1 under normal and double litter conditions, respectively) and the minimum in spring (0.27 and 0.69 μmol m−2 s−1 under normal and double litter conditions, respectively). On average, annual cumulative litter respiration was 115 and 300 g C m−2 y−1 under normal and double litter conditions, respectively. Using a soil temperature of 17°C as the critical point, the relationship between litter respiration and soil moisture was found to follow quadratic functions well, whereas the determination coefficient was much greater at high soil temperature than at low soil temperature (33–35% vs. 22–24%). Litter respiration was significantly higher in 2010 and 2012 than in 2011 under both normal litter (132–165 g C m−2 y−1 vs. 48 g C m−2 y−1) and double litter (389–418 g C m−2 y−1 vs. 93 g C m−2 y−1) conditions. Such significant interannual variations were largely ascribed to the differences in summer rainfall. Our study demonstrates that, apart from soil temperature, moisture also has significant influence on litter respiration in semi-arid regions. PMID:25474633

  18. Soil microbial community composition and respiration along an experimental precipitation gradient in a semiarid steppe

    PubMed Central

    Zhao, Cancan; Miao, Yuan; Yu, Chengde; Zhu, Lili; Wang, Feng; Jiang, Lin; Hui, Dafeng; Wan, Shiqiang

    2016-01-01

    As a primary limiting factor in arid and semiarid regions, precipitation strongly influences soil microbial properties. However, the patterns and mechanisms of soil microbial responses to precipitation have not been well documented. In this study, changes in soil microorganisms along an experimental precipitation gradient with seven levels of precipitation manipulation (i.e., ambient precipitation as a control, and ±20%, ±40%, and ±60% of ambient precipitation) were explored in a semiarid temperate steppe in northern China. Soil microbial biomass carbon and respiration as well as the ratio of fungal to bacterial biomass varied along the experimental precipitation gradient and peaked under the +40% precipitation treatment. The shifts in microbial community composition could be largely attributable to the changes in soil water and nutrient availability. The metabolic quotient increased (indicating reduced carbon use efficiency) with increasing precipitation due to the leaching of dissolved organic carbon. The relative contributions of microbial respiration to soil and ecosystem respiration increased with increasing precipitation, suggesting that heterotrophic respiration will be more sensitive than autotrophic respiration if precipitation increases in the temperate steppe as predicted under future climate-change scenarios. PMID:27074973

  19. Regulation of Boreal soil respiration: evidence from a Swedish forest fire chronosequence.

    NASA Astrophysics Data System (ADS)

    Mason, Kelly; Oakley, Simon; Ostle, Nicholas; DeLuca, Thomas; Arróniz-Crespo, María; Jones, Davey

    2014-05-01

    Globally, boreal forests occupy 14% of total land surface and are important regions for biogeochemical cycling of carbon (C) and nitrogen (N)1. They are recognised as stores of terrestrial C and reservoirs of uniquely adapted biodiversity. Like many forest biomes, boreal forests are under pressure from climate change and growing populations. C and N cycling in the boreal region is strongly influenced by the occurrence of forest fires, which return large amounts of stored N back into an otherwise N limited system2. The frequency and intensity of boreal forest fires is expected to increase in the next century as the global atmosphere warms and N deposition continues to increase due to human activities3,4. Despite the importance of these ecosystems, there is limited knowledge of the effects of interactions between climate and N limitation on soil respiration and feedbacks of carbon dioxide (CO2) and other greenhouse gases (GHGs) to the atmosphere. In this research we aimed to improve understanding of how changes in the frequency and intensity of fires might alter N and C dynamics in the boreal region. Specifically, we examined the degree of N limitation and the temperature sensitivity of GHG (CO2, N2O and CH4) fluxes from soils underlying carpets of Pleurozium schreberi, a feather moss known to form important symbiotic relationships with N-fixing cyanobacteria1, from a fire chronosequence of Swedish boreal forest stands. We hypothesised that: (1) soil respiration in late succession ecosystems is most N limited due to high soil C:N ratios and high microbial biomass; and (2) early succession forest soil respiration is most temperature sensitive due to higher N availability and higher bacterial biomass. To test these hypotheses, we took soil cores from a chronosequence of six sites in the northern boreal region of Sweden, including two early, two mid, and two late succession stands. These sites are dominated by mixed Pinus sylvestris and Picea abies, with an understory

  20. Microbial respiration per unit microbial biomass increases with carbon-to-nutrient ratios in soils

    NASA Astrophysics Data System (ADS)

    Spohn, Marie; Chodak, Marcin

    2015-04-01

    The ratio of carbon-to-nutrient in forest floors is usually much higher than the ratio of carbon-to-nutrient that soil microorganisms require for their nutrition. In order to understand how this mismatch affects carbon cycling, the respiration rate per unit soil microbial biomass carbon - the metabolic quotient (qCO2) - was studied. This was done in a field study (Spohn and Chodak, 2015) and in a meta-analysis of published data (Spohn, 2014). Cores of beech, spruce, and mixed spruce-beech forest soils were cut into slices of 1 cm from the top of the litter layer down to 5 cm in the mineral soil, and the relationship between the qCO2 and the soil carbon-to-nitrogen (C:N) and the soil carbon-to-phosphorus (C:P) ratio was analyzed. We found that the qCO2 was positively correlated with soil C:N ratio in spruce soils (R = 0.72), and with the soil C:P ratio in beech (R = 0.93), spruce (R = 0.80) and mixed forest soils (R = 0.96). We also observed a close correlation between the qCO2 and the soil C concentration in all three forest types. Yet, the qCO2 decreased less with depth than the C concentration in all three forest types, suggesting that the change in qCO2 is not only controlled by the soil C concentration. We conclude that microorganisms increase their respiration rate per unit biomass with increasing soil C:P ratio and C concentration, which adjusts the substrate to their nutritional demands in terms of stoichiometry. In an analysis of literature data, I tested the effect of the C:N ratio of soil litter layers on microbial respiration in absolute terms and per unit microbial biomass C. For this purpose, a global dataset on the microbial respiration rate per unit microbial biomass C - termed the metabolic quotient (qCO2) - was compiled form literature data. It was found that the qCO2 in the soil litter layers was positively correlated with the litter C:N ratio and negatively related with the litter nitrogen (N) concentration. The positive relation between the qCO2

  1. Response of soil respiration to experimental warming in a highland barley of the Tibet.

    PubMed

    Zhong, Zhi-Ming; Shen, Zhen-Xi; Fu, Gang

    2016-01-01

    Highland barley is an important dominant crop in the Tibet and the croplands of the Tibet are experiencing obvious climatic warming. However, information about how soil respiration will respond to climatic warming in the highland barley system is still lacking. A field warming experiment using infrared heaters with two warming magnitudes was conducted in a highland barley system of the Tibet in May 2014. Five daily cycles of soil respiration was measured using a CO2 flux system (Li-8100, Li-COR Biosciences, Lincoln, NE, USA) during the period from early June to early September in 2014. The high and low experimental warming significantly increased soil temperature by 1.98 and 1.52 °C over the whole study period, respectively. The high experimental warming significantly decreased soil moisture. Soil respiration and its temperature sensitivity did not significantly change under both the high and low experimental warming. The response of soil respiration to experimental warming did not linearly correlate with warming magnitudes because a greater experimental warming resulted in a higher soil drying. Our findings suggested that clarifying the response of soil CO2 production and its temperature sensitivity to climatic warming need consider water availability in the highland barley system of the Tibet. PMID:26933635

  2. Antecedent conditions influence soil respiration differences in shrub and grass patches

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Quantifying the response of soil respiration to past environmental conditions is critical for predicting how future climate and vegetation change will impact ecosystem carbon balance. Increased shrub dominance in semiarid grasslands has potentially large effects on soil carbon cycling. The goal of t...

  3. Soil respiration response to climate change in Pacific Northwest prairies is mediated by a regional Mediterranean climate gradient.

    PubMed

    Reynolds, Lorien L; Johnson, Bart R; Pfeifer-Meister, Laurel; Bridgham, Scott D

    2015-01-01

    Soil respiration is expected to increase with rising global temperatures but the degree of response may depend on soil moisture and other local factors. Experimental climate change studies from single sites cannot discern whether an observed response is site-dependent or generalizable. To deconvolve site-specific vs. regional climatic controls, we examined soil respiration for 18 months along a 520 km climate gradient in three Pacific Northwest, USA prairies that represents increasingly severe Mediterranean conditions from north to south. At each site we implemented a fully factorial combination of 2.5-3 °C warming and 20% added precipitation intensity. The response of soil respiration to warming was driven primarily by the latitudinal climate gradient and not site-specific factors. Warming increased respiration at all sites during months when soil moisture was not limiting. However, these gains were offset by reductions in respiration during seasonal transitions and summer drought due to lengthened periods of soil moisture limitation. The degree of this offset varied along the north-south climate gradient such that in 2011 warming increased cumulative annual soil respiration 28.6% in the northern site, 13.5% in the central site, and not at all in the southern site. Precipitation also stimulated soil respiration more frequently in the south, consistent with an increased duration of moisture limitation. The best predictors of soil respiration in nonlinear models were the Normalized Difference Vegetation Index (NDVI), antecedent soil moisture, and temperature but these models provided biased results at high and low soil respiration. NDVI was an effective integrator of climate and site differences in plant productivity in terms of their combined effects on soil respiration. Our results suggest that soil moisture limitation can offset the effect of warming on soil respiration, and that greater growing-season moisture limitation would constrain cumulative annual

  4. On the relative roles of hydrology, salinity, temperature, and root productivity in controlling soil respiration from coastal swamps (freshwater)

    USGS Publications Warehouse

    Krauss, Ken W.; Whitbeck, Julie L.; Howard, Rebecca J.

    2012-01-01

    Background and aims Soil CO2 emissions can dominate gaseous carbon losses from forested wetlands (swamps), especially those positioned in coastal environments. Understanding the varied roles of hydroperiod, salinity, temperature, and root productivity on soil respiration is important in discerning how carbon balances may shift as freshwater swamps retreat inland with sea-level rise and salinity incursion, and convert to mixed communities with marsh plants. Methods We exposed soil mesocosms to combinations of permanent flooding, tide, and salinity, and tracked soil respiration over 2 1/2 growing seasons. We also related these measurements to rates from field sites along the lower Savannah River, Georgia, USA. Soil temperature and root productivity were assessed simultaneously for both experiments. Results Soil respiration from mesocosms (22.7-1678.2 mg CO2 m-2 h-1) differed significantly among treatments during four of the seven sampling intervals, where permanently flooded treatments contributed to low rates of soil respiration and tidally flooded treatments sometimes contributed to higher rates. Permanent flooding reduced the overall capacity for soil respiration as soils warmed. Salinity did reduce soil respiration at times in tidal treatments, indicating that salinity may affect the amount of CO2 respired with tide more strongly than under permanent flooding. However, soil respiration related greatest to root biomass (mesocosm) and standing root length (field); any stress reducing root productivity (incl. salinity and permanent flooding) therefore reduces soil respiration. Conclusions Overall, we hypothesized a stronger, direct role for salinity on soil respiration, and found that salinity effects were being masked by varied capacities for increases in respiration with soil warming as dictated by hydrology, and the indirect influence that salinity can have on plant productivity.

  5. Soil respiration in pits and mounds following an experimental forest blowdown

    SciTech Connect

    Millikin, C.S.; Bowden, R.D.

    1996-11-01

    Extensive uprooting of trees by windthrow can create areas of severe soil disturbance in temperate forests. Specifically, uprooted trees leave shaded pits and mounds of exposed roots and mineral soil. To assess the contribution of pit and mound microenvironments to overall soil respiration in an experimental hurricane blowdown at the Harvard Forest Long-Term Ecological Research site (MA), summer CO{sub 2} effluxes were measured on pit, mound, and undisturbed microsites. Mean CO{sub 2} effluxes were 45.4, 80.1, and 99.0 mgC m{sup -2} h{sup -1} for pit, mound, and control microsites, respectively. Although soil respiration is lower in areas of disturbed soil than in undisturbed areas, the total efflux contribution (5.3%) form pits and mounds to the overall flux rate at the site was small. The area-weighted soil respiration estimate is 3.1% lower than the estimate obtained using flux measurements from control locations alone. Measurements taken from undisturbed plots represent a small but systematic overestimate of soil respiration across the site. 25 refs., 1 fig.

  6. Using Headspace Equilibration to Measure the d13C of Soil-Respired CO2

    NASA Astrophysics Data System (ADS)

    Robertson, M. A.; Powers, E.; Marshall, J.

    2007-12-01

    Soil respiration is an important component of the global carbon cycle and can account for as much as 70% of ecosystem respiration. Soil gas flux measurements have been combined with stable isotope analysis to examine ecosystem properties and processes such as water-use efficiency and the role of above ground weather in controlling soil respiration. However, current methods of measuring the δ13C of soil-respired CO2 are either inherently inaccurate or time-consuming and tedious. An alternative method of obtaining this value offers a potential solution to these problems. In this method, plastic chambers are fitted with rubber septa to allow for sample collection, then inverted and partially buried in soil. The chamber headspace is allowed to come to equilibrium with soil air. In this study we tested the viability of this method by examining whether frequent resampling of respiration chambers affected δ13C measurements, whether headspace CO2 concentration and δ13C values approached equilibrium asymptotically, and whether simulated and actual diel temperature cycles affected estimates of δ13C. All experiments were conducted on respiration chambers inverted in potting soil and placed in a Conviron growth chamber, with the exception of one field test that was conducted on respiration chambers installed in a Northern Idaho experimental forest. Samples were collected with a syringe and stored in glass vials for analysis by a ratioing mass spectrometer. We found that resampling respiration chambers as frequently as every 10 minutes had no significant effect on final δ13C values, that both chamber CO2 concentrations and δ13C values exhibited an asymptotic approach to equilibrium, and that the equilibrium value was offset from the initial flux by the amount we expected, approximately 4 ‰. However, we also found that diel temperature variation affected both headspace CO2 concentration and δ13C in the lab and in the field. We concluded that if this method is used in

  7. Bioirrigation impacts on sediment respiration and microbial metabolic activity

    NASA Astrophysics Data System (ADS)

    Baranov, V. A.; Lewandowski, J.; Romeijn, P.; Krause, S.

    2015-12-01

    Some bioturbators build tubes in the sediment and pump water through their burrows (ventilation). Oxygen is transferred through the burrow walls in the adjacent sediment (bioirrigation). Bioirrigation is playing a pivotal role in the mediation of biogeochemical processes in lake sediments and has the potential to enhance nutrient cycling. The present study investigates the impact of bioirrigation on lake sediment metabolism, respiration rates and in particular, the biogeochemical impacts of bioirrigation intensity as a function of organism density. We therefore apply the bioreactive Resazurin/Resorufin smart tracer system for quantifying the impact of different densities of Chironomidae (Diptera) larvae (0-2112 larvae/m2) on lake sediment respiration in a microcosm experiment. Tracer decay has been found to be proportional to the amount of the aerobic respiration at the sediment-water interface. Tracer transformation was in good agreement with Chironomidae density (correlation, r=0.9). Tracer transformation rates (and sediment respiration) were found to be correlated to Chironomidae density, with highest transformation rates observed in the microcosms with highest density of 2112 larvae/m2. This relationship was not linear though, with sediment respiration rates at the highest larvae densities declining from the linear trend predicted from lower and intermediate larvae density-respiration relationships. We interpret this effect as a density dependent suppression of the Chironomid's metabolic activity. The observations of this study have implications for eutrophied lakes with high densities of bioirrigators. Despite high density of bioirrigirrigating benthos, mineralization of the organic matter in such habitats would likely be lower than in lakes with intermediate densities of the bioturbators.

  8. Soil CO2 efflux in a sand grassland: contribution by root, mycorrhizal and basal respiration components

    NASA Astrophysics Data System (ADS)

    Papp, Marianna; Balogh, János; Pintér, Krisztina; Cserhalmi, Dóra; Nagy, Zoltán

    2014-05-01

    Grasslands play an important role in global carbon cycle because of their remarkable extension and carbon storage capacity. Soil respiration takes a major part in the carbon cycle of the ecosystems; ratio of its autotrophic and heterotrophic components is important also when considering their sensitivity to environmental drivers. The aim of the study was to estimate the contribution by root, mycorrhizal and basal components to total soil CO2 efflux. The study was carried out in the semi-arid sandy grassland dominated by Festuca pseudovina at the Kiskunság National Park in Hungary (Bugac site) where C-flux measurements have been going on since 2002. The soil CO2 effluxes were measured in the following treatments: a./ control, b./ root-exclusion, c./ root and mycorrhiza exclusion by using 80 cm long 15 cm inner diameter PVC tubes and micro-pore inox meshes. Inox mesh was used to exclude roots, but let the mycorrhiza filaments to grow into the tubes. 10 soil cores were excavated, sieved, then root-free soil was packed back layer by layer into the cores giving 6 and 4 repetitions in b and c treatments respectively. Basal respiration is referred to as the heterotrophic respiration without influence of roots or mycorrhiza. Difference between root-exclusion and root and mycorrhiza exclusion treatment gave the value of mycorrhizal respiration and control (non-disturbed) plots the total soil CO2 efflux. The contribution by the above components was evaluated. Soil CO2 efflux was measured continuously by using an automated open system of 10 soil respiration chambers. Data was collected in every two hours from each treatment (one of the chambers recorded basal respiration, 3 chambers were settled on root-excluded treatments and 6 chambers measured control plots). Chambers were moved in every 2 weeks between the repetitions of the treatments. Soil CO2 efflux (mycorrhiza-free, root free, control) data were fitted using a soil respiration model, where soil temperature, soil

  9. On-Line Isotopic Analysis of Soil-Respired CO2

    NASA Astrophysics Data System (ADS)

    Sulzman, E. W.; Rugh, W. D.; Crow, S. E.; Bowden, R. D.; Mix, A. C.; Lajtha, K.

    2004-12-01

    Soils from a replicated long-term litter manipulation study were collected from a Douglas fir stand in central Oregon and from a sugar maple-black cherry stand in northwestern Pennsylvania to determine if processing of labile and recalcitrant organic matter could be detected through changes in the isotopic signature of density-fractionated soil. Soils were density- fractionated at 1.6 g cm-3 using sodium polytungstate and incubated under controlled conditions (40% volumetric water content, 21\\deg C) for 65 days. The isotopic measurement of soil-respired CO2 was accomplished with a Finnigan Gas Bench II coupled to a Delta Plus XL Continuous Flow Mass Spectrometer; external precision was 0.06\\permil. We found significant isotopic (\\delta13C) differences in respired CO2 by density, but not among litter exclusion and addition treatments, even where the treatments have been in place for 11 years (PA). Microbially-mediated isotopic fractionation, expressed as the difference between substrate 13C and respired 13CO2, was large and positive the day of wet-up, but fell to near zero by day 5. Isotopic composition and patterns of lability (measured as respiration rate per gram C) varied dramatically between the two sites. The deciduous forest soil (Alfisol) yielded the expected pattern of higher lability and lower \\delta13CO2 values from the low density fraction. In contrast, the coniferous forest soil (Andisol) revealed a complicated pattern of higher lability in the high density fraction, suggesting organic matter composition, soil mineralogy, and microbiology are interacting in as yet unexplained ways in this soil. Overall, our findings suggest that density alone is not a clear indicator of the recalcitrance of soil organic matter, and that isotopes of respired CO2 may yield information about the processing of labile and recalcitrant organic matter.

  10. Comparison of soil respiration methods in a mid-latitude deciduous forest

    SciTech Connect

    Wayson, C. A.; Randolph, J. C.; Hanson, Paul J; Schmid, H. P.; Grimmond, CSB

    2006-01-01

    In forest ecosystems the single largest respiratory flux influencing net ecosystem productivity (NEP) is the total soil CO2 efflux; however, it is difficult to make measurements of this flux that are accurate at the ecosystem scale. We examined patterns of soil CO2 efflux using five different methods: auto-chambers, portable gas analyzers, eddy covariance along and two models parameterized with the observed data. The relation between soil temperature and soil moisture with soil CO2 effluxes are also investigated, both inter-annually and seasonally, using these observations/results. Soil respiration rates (Rsoil) are greatest during the growing season when soil temperatures are between 15 and 25 C, but some soil CO2 efflux occurs throughout the year. Measured soil respiration was sensitive to soil temperature, particularly during the spring and fall. All measurement methods produced similar annual estimates. Depending on the time of the year, the eddy covariance (flux tower) estimate for ecosystem respiration is similar to or slightly lower than estimates of annual soil CO2 efflux from the other methods. As the eddy covariance estimate includes foliar and stem respiration which the other methods do not; it was expected to be larger (perhaps 15-30%). The auto-chamber system continuously measuring soil CO2 efflux rates provides a level of temporalr esolution that permits investigation of short- to longer term influences of factors on these efflux rates. The expense of building and maintaining an auto chamber system may not be necessary for those esearchers interested in estimating Rsoil annually, but auto-chambers do allow the capture of data from all seasons needed for model parameterization.

  11. Estimating sources of winter soil respiration in a subalpine forest using a hierarchical Bayesian process-based stable isotope mixing model

    NASA Astrophysics Data System (ADS)

    Tucker, C.; Ogle, K.; Cable, J.

    2011-12-01

    Recent studies show that snow-covered soils in subalpine forest ecosystems may support high levels of biological activity and significant soil respiration. Most winter soil respiration has been attributed to soil microbial activity, but very little work has been conducted to quantify plant root activity during this time period. The lack of such data may reflect common assumptions about over-winter plant dormancy, leading to the expectation that plant roots are inactive during the winter. In this study, we quantify autotrophic (roots and root-associated microbes) and heterotrophic (free-living microbes and soil fauna) respiration, across four (2008-2011) winter seasons in a subalpine forest in Wyoming. We implement a novel hierarchical Bayesian (HB) model that combines a 13C-CO2 stable isotope mixing model with a process-based model of soil heterotrophic and autotrophic temperature responses to facilitate partitioning total respiration between these sources. In particular, the HB approach simultaneously integrates field data on snowpack CO2 concentration and isotope gradients, snowpack and soil physical characteristics (i.e., temperature, moisture, density), root and microbial biomass, soil carbon, and data obtained from laboratory incubations of roots and soils. The process model components include temperature constraints on root and microbial activity, a Michaelis-Menten-type model for microbial respiration in response to substrate limitation, and a diffusion driven model of CO2 transport through the snow. Averaging across years, soil respiration increased from 0.35 μmol CO2 m-2 s-1 in January to 0.6 μmol CO2 m-2 s-1 in May while snow depth increased and soil temperature remained stable over the same period. The increase in respiration appeared to be driven by a two- to four-fold increase in microbial biomass carbon as winter progressed. Carbon limitation of microbial activity during the winter appears to be negligible, and we suggest that high carbon use

  12. Decadal Effects of Elevated CO2 and O3 on Forest Soil Respiration and Belowground Carbon Cycling at Aspen FACE

    NASA Astrophysics Data System (ADS)

    Talhelm, A. F.; Pregitzer, K. S.; Zak, D. R.; Burton, A. J.

    2014-12-01

    Three northern temperate forest communities in the north-central United States were exposed to factorial combinations of elevated carbon dioxide (CO2) and/or tropospheric ozone (O3) for 11 years, advancing from open-grown seedlings <0.25 m in height at the beginning to the experiment to closed-canopy stands that were >8 m tall. Here, we report results from measurements of soil respiration that occurred during the experiment from 1999 to 2008. In order to better understand this flux, we compare changes in soil respiration to the effects of CO2 and O3 on net primary productivity (NPP), fine root biomass, and leaf litter production. Elevated CO2 enhanced soil respiration by an average of 28%. This stimulation of soil respiration varied from +19% to +44%, but did not change consistently during the 10 year measurement period (r2 = 0.04). The effect of elevated O3 on soil respiration was dynamic. In year two of the experiment (1999), elevated O3 decreased soil respiration by 7%. However, soil respiration consistently increased through time under elevated O3 (r2 = 0.71) and was 9% greater than under ambient O3 in the final year of the experiment (2008). Overall, elevated O3 had no meaningful effect on soil respiration (+0.3%). The annual effects of elevated CO2 on soil respiration were not correlated with NPP or fine root biomass, but was positively correlated with leaf litter production (r = 0.57). Annual leaf litter production was also related to the annual effects of elevated O3 on soil respiration (r = 0.78), but relationship was tighter between annual O3 effects on NPP and soil respiration (r = 0.83).

  13. Environmental forcing does not lead to variation in carbon isotope content of forest soil respiration

    NASA Astrophysics Data System (ADS)

    Bowling, David; Egan, Jocelyn; Hall, Steven; Risk, David

    2015-04-01

    Recent studies have highlighted fluctuations in the carbon isotope content (δ13C) of CO2 produced by soil respiration. These have been correlated with diel cycles of environmental forcing (e.g., soil temperature), or with synoptic weather events (e.g., rain events and pressure-induced ventilation). We used an extensive suite of observations to examine these phenomena over two months in a subalpine forest in Colorado, USA (the Niwot Ridge AmeriFlux site). Measurements included automated soil respiration chambers and automated measurements of the soil gas profile. We found 1) no diel change in the δ13C of the soil surface flux or the CO2 produced in the soil (despite strong diel change in surface flux rate), 2) no change in δ13C following wetting (despite a significant increase in soil flux rate), and 3) no evidence of pressure-induced ventilation of the soil. Measurements of the δ13C of surface CO2 flux agreed closely with the isotopic composition of soil CO2 production calculated using soil profile measurements. Temporal variation in the δ13C of surface flux was relatively minor and unrelated to measured environmental variables. Deep in the soil profile, results conform to established theory regarding diffusive soil gas transport and isotopic fractionation, and suggest that sampling soil gas at a depth of several tens of centimeters is a simple and effective way to assess the mean δ13C of the surface flux.

  14. A parameterization of respiration in frozen soils based on substrate availability

    NASA Astrophysics Data System (ADS)

    Schaefer, K.; Jafarov, E.

    2015-07-01

    Respiration in frozen soils is limited to thawed substrate within the thin water films surrounding soil particles. As temperatures decrease and the films become thinner, the available substrate also decreases, with respiration effectively ceasing at -8 °C. Traditional exponential scaling factors to model this effect do not account for substrate availability and do not work at the century to millennial time scales required to model the fate of the nearly 1700 Gt of carbon in permafrost regions. The exponential scaling factor produces a false, continuous loss of simulated permafrost carbon in the 20th century and biases in estimates of potential emissions as permafrost thaws in the future. Here we describe a new frozen biogeochemistry parameterization that separates the simulated carbon into frozen and thawed pools to represent the effects of substrate availability. We parameterized the liquid water fraction as a function of temperature based on observations and use this to transfer carbon between frozen pools and thawed carbon in the thin water films. The simulated volumetric water content (VWC) as a function of temperature is consistent with observed values and the simulated respiration fluxes as a function of temperature are consistent with results from incubation experiments. The amount of organic matter was the single largest influence on simulated VWC and respiration fluxes. Future versions of the parameterization should account for additional, non-linear effects of substrate diffusion in thin water films on simulated respiration. Controlling respiration in frozen soils based on substrate availability allows us to maintain a realistic permafrost carbon pool by eliminating the continuous loss caused by the original exponential scaling factors. The frozen biogeochemistry parameterization is a useful way to represent the effects of substrate availability on soil respiration in model applications that focus on century to millennial time scales in permafrost

  15. A parameterization of respiration in frozen soils based on substrate availability

    NASA Astrophysics Data System (ADS)

    Schaefer, Kevin; Jafarov, Elchin

    2016-04-01

    Respiration in frozen soils is limited to thawed substrate within the thin water films surrounding soil particles. As temperatures decrease and the films become thinner, the available substrate also decreases, with respiration effectively ceasing at -8 °C. Traditional exponential scaling factors to model this effect do not account for substrate availability and do not work at the century to millennial timescales required to model the fate of the nearly 1100 Gt of carbon in permafrost regions. The exponential scaling factor produces a false, continuous loss of simulated permafrost carbon in the 20th century and biases in estimates of potential emissions as permafrost thaws in the future. Here we describe a new frozen biogeochemistry parameterization that separates the simulated carbon into frozen and thawed pools to represent the effects of substrate availability. We parameterized the liquid water fraction as a function of temperature based on observations and use this to transfer carbon between frozen pools and thawed carbon in the thin water films. The simulated volumetric water content (VWC) as a function of temperature is consistent with observed values and the simulated respiration fluxes as a function of temperature are consistent with results from incubation experiments. The amount of organic matter was the single largest influence on simulated VWC and respiration fluxes. Future versions of the parameterization should account for additional, non-linear effects of substrate diffusion in thin water films on simulated respiration. Controlling respiration in frozen soils based on substrate availability allows us to maintain a realistic permafrost carbon pool by eliminating the continuous loss caused by the original exponential scaling factors. The frozen biogeochemistry parameterization is a useful way to represent the effects of substrate availability on soil respiration in model applications that focus on century to millennial timescales in permafrost regions.

  16. A multi-time scale, non-linear approach to understanding soil respiration

    NASA Astrophysics Data System (ADS)

    Nickerson, N. R.; Phillips, C.; Risk, D. A.

    2010-12-01

    To understand the processes that drive soil respiration and to make accurate predictions about global carbon cycling and potential climate feedbacks, it is critical that we develop accurate models that are useful on a range of timescales. There is, however, little agreement on the functional form and parameters that should be associated with modeling total soil respiration. Field data provides the most realistic platform for this assessment, but the environmental controls on soil respiration have been difficult to estimate in the field with good accuracy due to a combination of factors, including: (1) physical and biological uncertainties that are present in the field (ie. heat and gas diffusion, nutrient and substrate limitation); (2) the absence of a standardized and theoretically sound method for calculating model parameters using field data, and; (3) the absence of suitable long term, high temporal resolution respiration data from field studies, which is now becoming more available. This research focuses on multi-time scale non-linear analysis techniques, and their role in guiding the development of new soil respiration models that accurately predict respiration on a range of timescales. Using a physical model as a proxy of real world conditions, we focus on the confounding effect of physical factors, such as heat and gas diffusion and CO2 production depth, which have been found to be the cause of a considerable amount of error in past studies. Preliminary results show that for estimating temperature sensitivity, the non-linear approach is the best (compared to the typical log transform linear approach) in all circumstances, although caution should be exercised when analyzing short time series (i.e. diel) data because the lag and damping cause by gas diffusion may affect estimates. This work also examines moisture sensitivity parameters and the confounding effects of moisture on temperature sensitivity estimates. Finally we provide an evaluation of temporal

  17. Controls on Soil Respiration in a High Elevation Alpine System and the Implications For Soil Carbon Storage in a Changing Climate

    NASA Astrophysics Data System (ADS)

    Schliemann, S. A.

    2015-12-01

    The alpine ecosystem is a dynamic network of heterogeneous soil and vegetation patches. Microsite characteristics are controlled by site geomorphology, underlying bedrock, and landscape position. These microsite characteristics create a complex mosaic of soil moisture and temperature regimes across the landscape. To investigate the relative influences of soil moisture and soil temperature on soil respiration in these varied microsites, 12 study sites were established in June of 2015 in Rocky Mountain National Park, Colorado. Sites were distributed across 3 plots with distinct vegetation and soil regimes: 1) Conifer forest at the upper limit of the tree line 2) Tundra characterized by shallow soil and minimal vegetation consisting of herbs and lichen 3) Tundra characterized by organic-rich, deep soil and abundant vegetation consisting of grasses and sedges. Soil respiration, soil temperature, and soil moisture were measured weekly throughout the snow-free period of 2015. Soil moisture was negatively correlated with soil respiration and soil temperature was positively correlated with soil respiration across the study sites (p <0.001). Soil respiration rates were significantly different from one another in all plots and were highest in the forest plot (maximum 9.6 μmol/ m2/sec) and much lower in the two tundra plots (< 4.5 μmol/ m2/sec) (p < 0.001). These data suggest that as the alpine climate warms, an increase in soil temperature and a longer snow-free period may result in an overall increase in the rate of soil respiration, which could alter the soil carbon pool. In addition, as temperatures rise, the tree line may migrate to a higher elevation. The results of this study suggest that with such a movement, the soil respiration rate will also increase. However the net change in soil organic matter in the newly established forest would not only depend on the soil respiration rate, but on the overall capacity of the new forest soil to retain carbon, especially

  18. Soil carbon storage and respiration potential across a landscape age and climate gradient in western Greenland

    NASA Astrophysics Data System (ADS)

    Bradley-Cook, J. I.; Virginia, R. A.; Hammond Wagner, C.; Racine, P. E.

    2013-12-01

    The soil formation state factors proposed by Hans Jenny (climate, organisms, relief, parent material, time) explain many soil characteristics, yet geological controls on biological carbon cycling are not well represented in regional carbon models. Landscape age, for instance, can directly affect the quantity and quality of soil organic carbon, which are key determinants of the temperature sensitivity of soil organic matter (SOM) to decomposition. Temperature control of SOM decomposition is of particular importance in Arctic soils, which contain nearly half of global belowground organic carbon and have a permafrost thermal regime that straddles the freeze-thaw threshold. We investigated soil carbon storage and respiration potential across a west Greenland transect, and related the landscape carbon patterns to regional variation in climate and landscape age. The four study sites capture a range in: landscape age from 180 years on the inland Little Ice Age moraine near Kangerlussuaq to ~10,000 years at the coastal sites near Sisimiut and Nuuk, mean annual air temperatures from -5.7 to -1.4 °C, and mean annual precipitation from 149 to 752 mm. At each site, we collected surface and mineral samples from nine soil pits within similar vegetation cover and relief classes. We measured total organic carbon and nitrogen though elemental analysis, and incubated soils at 4 °C and field capacity moisture for 175 day to measure carbon dioxide production from which we derived soil respiration potential. We hypothesized that soil carbon storage and respiration potential would be greatest at the sites with the oldest landscape age. Soil carbon content was more than four times greater at the 10,000 year sites (Nuuk = 24.03%, Sisimiut = 17.34%) than the inland sites (Ørkendalen = 3.49%, LIA = 0.05%). Carbon quality decreased across the age gradient, as measured by a nearly two-fold increase in C:N ratio from the youngest and driest to the oldest and wettest soils (LIA = 12.2, Nuuk

  19. Seasonal and episodic moisture controls on plant and microbial contributions to soil respiration.

    PubMed

    Carbone, Mariah S; Still, Christopher J; Ambrose, Anthony R; Dawson, Todd E; Williams, A Park; Boot, Claudia M; Schaeffer, Sean M; Schimel, Joshua P

    2011-09-01

    Moisture inputs drive soil respiration (SR) dynamics in semi-arid and arid ecosystems. However, determining the contributions of root and microbial respiration to SR, and their separate temporal responses to periodic drought and water pulses, remains poorly understood. This study was conducted in a pine forest ecosystem with a Mediterranean-type climate that receives seasonally varying precipitation inputs from both rainfall (in the winter) and fog-drip (primarily in the summer). We used automated SR measurements, radiocarbon SR source partitioning, and a water addition experiment to understand how SR, and its separate root and microbial sources, respond to seasonal and episodic changes in moisture. Seasonal changes in SR were driven by surface soil water content and large changes in root respiration contributions. Superimposed on these seasonal patterns were episodic pulses of precipitation that determined the short-term SR patterns. Warm season precipitation pulses derived from fog-drip, and rainfall following extended dry periods, stimulated the largest SR responses. Microbial respiration dominated these SR responses, increasing within hours, whereas root respiration responded more slowly over days. We conclude that root and microbial respiration sources respond differently in timing and magnitude to both seasonal and episodic moisture inputs. These findings have important implications for the mechanistic representation of SR in models and the response of dry ecosystems to changes in precipitation patterns. PMID:21487825

  20. Rates of Litter Decomposition and Soil Respiration in Relation to Soil Temperature and Water in Different-Aged Pinus massoniana Forests in the Three Gorges Reservoir Area, China

    PubMed Central

    Zeng, Lixiong; Huang, Zhilin; Lei, Jingpin; Zhou, Benzhi; Li, Maihe

    2014-01-01

    To better understand the soil carbon dynamics and cycling in terrestrial ecosystems in response to environmental changes, we studied soil respiration, litter decomposition, and their relations to soil temperature and soil water content for 18-months (Aug. 2010–Jan. 2012) in three different-aged Pinus massoniana forests in the Three Gorges Reservoir Area, China. Across the experimental period, the mean total soil respiration and litter respiration were 1.94 and 0.81, 2.00 and 0.60, 2.19 and 0.71 µmol CO2 m−2 s−1, and the litter dry mass remaining was 57.6%, 56.2% and 61.3% in the 20-, 30-, and 46-year-old forests, respectively. We found that the temporal variations of soil respiration and litter decomposition rates can be well explained by soil temperature at 5 cm depth. Both the total soil respiration and litter respiration were significantly positively correlated with the litter decomposition rates. The mean contribution of the litter respiration to the total soil respiration was 31.0%–45.9% for the three different-aged forests. The present study found that the total soil respiration was not significantly affected by forest age when P. masonniana stands exceed a certain age (e.g. >20 years old), but it increased significantly with increased soil temperature. Hence, forest management strategies need to protect the understory vegetation to limit soil warming, in order to reduce the CO2 emission under the currently rapid global warming. The contribution of litter decomposition to the total soil respiration varies across spatial and temporal scales. This indicates the need for separate consideration of soil and litter respiration when assessing the climate impacts on forest carbon cycling. PMID:25004164

  1. Microbial biomass and basal respiration in Sub-Antarctic and Antarctic soils in the areas of some Russian polar stations

    NASA Astrophysics Data System (ADS)

    Abakumov, E.; Mukhametova, N.

    2014-03-01

    Antarctica is the unique place for pedological investigations. Soils of Antarctica have been studied intensively during the last century. Antarctic logistic provides the possibility to scientists access the terrestrial landscapes mainly in the places of polar stations. That is why the main and most detailed pedological investigations were conducted in Mc Murdo Valleys, Transantarctic Mountains, South Shetland Islands, Larsemann hills and Schirmacher Oasis. Investigations were conducted during the 53rd and 55th Russian Antarctic expeditions on the base of soil pits and samples collected in Sub-Antarctic and Antarctic regions. Soils of diverse Antarctic landscapes were studied with aim to assess the microbial biomass level, basal respiration rates and metabolic activity of microbial communities. The investigation conducted shows that soils of Antarctic are quite different in profile organization and carbon content. In general, Sub-Antarctic soils are characterized by more developed humus (sod) organo-mineral horizons as well as the upper organic layer. The most developed organic layers were revealed in peat soils of King-George Island, where its thickness reach even 80 cm. These soils as well as soils under guano are characterized by the highest amount of total organic carbon (TOC) 7.22-33.70%. Coastal and continental soils of Antarctic are presented by less developed Leptosols, Gleysols, Regolith and rare Ornhitosol with TOC levels about 0.37-4.67%. The metabolic ratios and basal respiration were higher in Sub-Antarctic soils than in Antarctic ones which can be interpreted as result of higher amounts of fresh organic remnants in organic and organo-mineral horizons. Also the soils of King-George island have higher portion of microbial biomass (max 1.54 mg g-1) than coastal (max 0.26 mg g-1) and continental (max 0.22 mg g-1) Antarctic soils. Sub-Antarctic soils mainly differ from Antarctic ones in increased organic layers thickness and total organic carbon content

  2. Influence of vestibular activation on respiration in humans

    NASA Technical Reports Server (NTRS)

    Monahan, Kevin D.; Sharpe, Melissa K.; Drury, Daniel; Ertl, Andrew C.; Ray, Chester A.

    2002-01-01

    The purpose of this study was to determine the effects of the semicircular canals and otolith organs on respiration in humans. On the basis of animal studies, we hypothesized that vestibular activation would elicit a vestibulorespiratory reflex. To test this hypothesis, respiratory measures, arterial blood pressure, and heart rate were measured during engagement of semicircular canals and/or otolith organs. Dynamic upright pitch and roll (15 cycles/min), which activate the otolith organs and semicircular canals, increased respiratory rate (Delta2 +/- 1 and Delta3 +/- 1 breaths/min, respectively; P < 0.05). Dynamic yaw and lateral pitch (15 cycles/min), which activate the semicircular canals, increased respiration similarly (Delta3 +/- 1 and Delta2 +/- 1, respectively; P < 0.05). Dynamic chair rotation (15 cycles/min), which mimics dynamic yaw but eliminates neck muscle afferent, increased respiration (Delta3 +/- 1; P < 0.05) comparable to dynamic yaw (15 cycles/min). Increases in respiratory rate were graded as greater responses occurred during upright (Delta5 +/- 2 breaths/min) and lateral pitch (Delta4 +/- 1) and roll (Delta5 +/- 1) performed at 30 cycles/min. Increases in breathing frequency resulted in increases in minute ventilation during most interventions. Static head-down rotation, which activates otolith organs, did not alter respiratory rate (Delta1 +/- 1 breaths/min). Collectively, these data indicate that semicircular canals, but not otolith organs or neck muscle afferents, mediate increased ventilation in humans and support the concept that vestibular activation alters respiration in humans.

  3. Effects of Forest Age on Soil Autotrophic and Heterotrophic Respiration Differ between Evergreen and Deciduous Forests

    PubMed Central

    Wang, Wei; Zeng, Wenjing; Chen, Weile; Yang, Yuanhe; Zeng, Hui

    2013-01-01

    We examined the effects of forest stand age on soil respiration (SR) including the heterotrophic respiration (HR) and autotrophic respiration (AR) of two forest types. We measured soil respiration and partitioned the HR and AR components across three age classes ∼15, ∼25, and ∼35-year-old Pinus sylvestris var. mongolica (Mongolia pine) and Larix principis-rupprechtii (larch) in a forest-steppe ecotone, northern China (June 2006 to October 2009). We analyzed the relationship between seasonal dynamics of SR, HR, AR and soil temperature (ST), soil water content (SWC) and normalized difference vegetation index (NDVI, a plant greenness and net primary productivity indicator). Our results showed that ST and SWC were driving factors for the seasonal dynamics of SR rather than plant greenness, irrespective of stand age and forest type. For ∼15-year-old stands, the seasonal dynamics of both AR and HR were dependent on ST. Higher Q10 of HR compared with AR occurred in larch. However, in Mongolia pine a similar Q10 occurred between HR and AR. With stand age, Q10 of both HR and AR increased in larch. For Mongolia pine, Q10 of HR increased with stand age, but AR showed no significant relationship with ST. As stand age increased, HR was correlated with SWC in Mongolia pine, but for larch AR correlated with SWC. The dependence of AR on NDVI occurred in ∼35-year-old Mongolia pine. Our study demonstrated the importance of separating autotrophic and heterotrophic respiration components of SR when stimulating the response of soil carbon efflux to environmental changes. When estimating the response of autotrophic and heterotrophic respiration to environmental changes, the effect of forest type on age-related trends is required. PMID:24282560

  4. [Effects of Reduced Water and Diurnal Warming on Winter-Wheat Biomass and Soil Respiration].

    PubMed

    Wu, Yang-zhou; Chen, Jian; Hu, Zheng-hua; Xie, Yan; Chen, Shu-tao; Zhang, Xue-song; Shen, Shuang-he; Chen, Xi

    2016-01-15

    Field experiments were conducted in winter wheat-growing season to investigate the effect of reduced water and diurnal warming on wheat biomass and soil respiration. The experimental treatments included the control (CK), 30% reduced water (W), diurnal warming (T, enhanced 2 degrees C), and the combined treatment (TW, 30% reduced water plus diurnal warming 2 degrees C). Soil respiration rate was measured using a static chamber-gas chromatograph technique. The results showed that in the winter wheat-growing season, compared to CK, T and TW treatments significantly increased shoot biomass by 46.0% (P = 0.002) and 19.8% (P = 0.032) during the elongation-booting stage, respectively. T and TW treatments also significantly increased the harvested shoot biomass by 19.8% (P = 0.050) and 34.6% (P = 0.028), respectively. On the other hand, W treatment had no significant effect on shoot biomass, and W, T, and TW treatments didn't significantly change the root biomass. T and W treatments had no significant effect on the mean respiration rate (MRR) of soil (P > 0.05). TW treatment significantly decreased soil MRR by 22.4% (P = 0.049). We also found T treatment decreased the temperature sensitivity coefficients of soil respiration (Q10). The results of our study suggested that compared to the single treatment (reduced water or diurnal warming), the combined treatment (reduced water plus diurnal warming) may have different effects on agroecosystem. PMID:27078968

  5. Root and microbial respiration from urban, agricultural and natural soils within the Moscow megapolis

    NASA Astrophysics Data System (ADS)

    Vasenev, Viacheslav; Castaldi, Simona; Vizirskaya, Marya; Ananyeva, Nadezhda; Ivashchenko, Kristina; Valentini, Riccardo; Vasenev, Ivan

    2015-04-01

    Urbanization is an important process of land-use change, which is increasing with the growth of population and abandonment of rural areas. Urbanization alters profoundly soil features and functions, among which soil respiration, which is one of the main carbon fluxes to the atmosphere. Soil respiration is the result of heterotrophic and autotrophic components, which are driven by biotic and abiotic factors. Little is known about soil respiration and its components in urban environments, which represent highly variable systems, characterized by different functional zones, types and intensities of urban management. In the present study we analyzed the spatial variability and temporal dynamics of total soil respiration (Rs) and its components, autotrophic (Ra) and heterotrophic respiration (Rh), from soils of different environments included in the Moscow megalopolis area. In particular we compared highly impacted areas urban green lawns with less anthropized ecosystems within the Moscow city: arable lands and urban forest sites. Experiments were set after snow melt and respiration fluxes were analyzed during the whole summer period till the beginning of the autumn. Data showed that Rs was significantly higher in the most disturbed sites, the green lawns, and showed the highest variability among the three analyzed land use types. Rh was the dominant component of soil respiration in all sites and did not vary significantly during the study period. However, significant differences was shown for the metabolic quotient qCO2, estimated as heterotrophic respiration ratio to microbial carbon (Rh/Cmic). The most disturbed sites showed the highest qCO2 within the lawn land use, followed by arable sites and forest sites, characterized by the lowest qCO2. Ra contributed to total Rs only at a minor extent (26%) and increased in all study sites along the season following the phenological cycle of the plant communities. Ra absolute values and relative contribution to Rs did not

  6. Contrasting diel hysteresis between soil autotrophic and heterotrophic respiration in a desert ecosystem under different rainfall scenarios

    PubMed Central

    Song, Weimin; Chen, Shiping; Zhou, Yadan; Wu, Bo; Zhu, Yajuan; Lu, Qi; Lin, Guanghui

    2015-01-01

    Diel hysteresis occurs often between soil CO2 efflux (RS) and temperature, yet, little is known if diel hysteresis occurs in the two components of RS, i.e., autotrophic respiration (RA) and heterotrophic respiration (RH), and how diel hysteresis will respond to future rainfall change. We conducted a field experiment in a desert ecosystem in northern China simulating five different scenarios of future rain regimes. Diel variations of soil CO2 efflux and soil temperature were measured on Day 6 and Day 16 following the rain addition treatments each month during the growing season. We found contrasting responses in the diel hysteresis of RA and RH to soil temperature, with a clockwise hysteresis loop for RH but a counter-clockwise hysteresis loop for RA. Rain addition significantly increased the magnitude of diel hysteresis for both RH and RA on Day 6, but had no influence on either on Day 16 when soil moisture was much lower. These findings underline the different roles of biological (i.e. plant and microbial activities) and physical-chemical (e.g. heat transport and inorganic CO2 exchange) processes in regulating the diel hysteresis of RA and RH, which should be considered when estimating soil CO2 efflux in desert regions under future rainfall regime. PMID:26615895

  7. Contrasting diel hysteresis between soil autotrophic and heterotrophic respiration in a desert ecosystem under different rainfall scenarios.

    PubMed

    Song, Weimin; Chen, Shiping; Zhou, Yadan; Wu, Bo; Zhu, Yajuan; Lu, Qi; Lin, Guanghui

    2015-01-01

    Diel hysteresis occurs often between soil CO2 efflux (R(S)) and temperature, yet, little is known if diel hysteresis occurs in the two components of R(S), i.e., autotrophic respiration (R(A)) and heterotrophic respiration (R(H)), and how diel hysteresis will respond to future rainfall change. We conducted a field experiment in a desert ecosystem in northern China simulating five different scenarios of future rain regimes. Diel variations of soil CO2 efflux and soil temperature were measured on Day 6 and Day 16 following the rain addition treatments each month during the growing season. We found contrasting responses in the diel hysteresis of R(A) and R(H) to soil temperature, with a clockwise hysteresis loop for R(H) but a counter-clockwise hysteresis loop for R(A). Rain addition significantly increased the magnitude of diel hysteresis for both R(H) and R(A) on Day 6, but had no influence on either on Day 16 when soil moisture was much lower. These findings underline the different roles of biological (i.e. plant and microbial activities) and physical-chemical (e.g. heat transport and inorganic CO2 exchange) processes in regulating the diel hysteresis of R(A) and R(H), which should be considered when estimating soil CO2 efflux in desert regions under future rainfall regime. PMID:26615895

  8. Winter soil CO2 efflux and its contribution to annual soil respiration in different ecosystems of Ebinur Lake Area

    NASA Astrophysics Data System (ADS)

    Qin, L.; Lv, G. H.; He, X. M.; Yang, J. J.; Wang, H. L.; Zhang, X. N.; Ma, H. Y.

    2015-08-01

    Arid and semiarid areas account for about one-third of the total land surface, and which play an important role in the global carbon cycle and climate system. However, up to now, compare with plenty knowledge information on winter soil efflux of forest ecosystems in mid-latitude ecosystems, winter soil efflux of arid areas at mid-latitude ecosystems is scare, Ebinur Lake Area, which is the study area of the present study, is located in arid regions of Northern China, with a vulnerable ecological environment suffering from extreme weather and climate. The objectives of this study were: (1) measure the winter soil respiration rate in our study area and determine its major environmental factors; (2) determine the winter soil CO2 efflux and its contribution to annual soil CO2 efflux in different ecosystems; and (3) discuss the estimated method of soil respiration that is most suitable to arid areas. We measured winter soil CO2 efflux and the associated environment factors in a farmland ecosystem (50a and 9a cotton fields), an abandoned land ecosystem (7a and 3a abandoned lands) and desert ecosystem ( Populus euphratica, Phragmites australis communities and sandy desert) in Ebinur Lake Area, China. The average winter soil respiration rate in the arid areas in the mid-latitude was 0.063 μmol m-2 s-1 to 0.730 μmol m-2 s-1. Specifically, the average winter soil respiration rate in the farmland ecosystems, abandoned land ecosystems and desert ecosystems were 0.686 μmol m-2 s-1, 0.443 μmol m-2 s-1 and 0.276 μmol m-2 s-1, respectively. Range of annual Q 10 (known as the increase in soil respiration rate per 10°C increase in temperature) in the three ecosystems were 0.989 to 4.962, 1.971 to 2.096 and 0.947 to 5.173, respectively. The relatively higher Q 10 values in the different ecosystems were all obtained in winter. We found that water (in the form of soil moisture or atmospheric humidity) was the primary factor that affected the change of soil respiration rate in the

  9. Respiration-to-DNA ratio reflects physiological state of microorganisms in root-free and rhizosphere soil

    NASA Astrophysics Data System (ADS)

    Blagodatskaya, E.; Blagodatsky, S.; Kuzyakov, Y.

    2009-04-01

    VCO2-to-total DNA ratios were lower than 0.1 µg CO2-C µg-1 total DNA h-1 whereas during exponential microbial growth these values increased consistently and exceeded 1 µg CO2-C µg-1 DNA h-1. Thus, the VCO2-to-total DNA ratio strongly changes along with the physiological state of soil microorganisms and can be used as valuable physiological parameter. In growing microorganisms the quantity of CO2 evolved per unit of newly formed DNA was identical in rhizosphere and root free soil and averaged for 13.5 ± 1.1 µg CO2-C µg-1 newly formed DNA. The CO2 yield per unit of newly formed DNA allows the estimation of microbial growth efficiency and validation of specific growth rates obtained during kinetic analysis of respiration curves. The study was supported by European Commission (Marie Curie IIF program, project MICROSOM) and by Alexander von Humboldt Foundation. References: Blagodatskaya EV, Blagodatskii SA, Anderson TH. 2003. Quantitative Isolation of Microbial DNA from Different Types of Soils of Natural and Agricultural Ecosystems. Microbiology 72(6):744-749. Blagodatsky SA, Heinemeyer O, Richter J. 2000. Estimating the active and total soil microbial biomass by kinetic respiration analysis. Biology and Fertility of Soils 32(1):73-81.

  10. Mountain pine beetle disturbance effects on soil respiration and nutrient pools

    NASA Astrophysics Data System (ADS)

    Trahan, N. A.; Moore, D. J.; Brayden, B. H.; Dynes, E.; Monson, R. K.

    2011-12-01

    Over the past decade, the mountain pine beetle Dendroctonos ponderosae has infested more than 86 million hectares of high elevation forest in the Western U.S.A. While bark beetles are endemic to western forests and important agents of regeneration, the current mountain pine beetle outbreak is larger than any other on record and the resulting tree mortality has significant consequences for nutrient cycling and regional carbon exchange. We established decade-long parallel disturbance chronosequences in two lodgepole pine (Pinus contorta) forests in Colorado: one composed of mountain pine beetle killed lodgepole stands and one consisting of trees where beetle mortality was simulated by stem girdling. Over the 2010 and 2011 growing season we measured plot level soil respiration fluxes, as well as soil extractable dissolved organic carbon, nitrogen, microbial biomass carbon and nitrogen, and pools of ammonium, nitrate and inorganic phosphorus. We show that soil respiration sharply declines with gross primary productivity after tree mortality, but rebounds during the next 4 years, then declines again from 6-8 years post-disturbance. Soil extractable dissolved organic carbon, microbial biomass carbon, and inorganic phosphorous pools follow the pattern observed in soil respiration fluxes across disturbance age classes for both sites, while patterns in total dissolved nitrogen exhibit site specific variation. Levels of detectable soil nitrate were low and did not significantly change across the chronosequence, while soil ammonium increased in a similar pattern with soil moisture in disturbed plots. These patterns in soil respiration and nutrient pools reflect the loss of autotrophic respiration and rhizodeposition immediately after tree mortality, followed by a pulse in soil efflux linked to the decomposition of older, less labile carbon pools. This pulse is likely controlled by the fall rate of litter, coarse woody debris and the relative impact of post-disturbance water

  11. Effects of Simulated Nitrogen Deposition on Soil Respiration in a Populus euphratica Community in the Ebinur Lake Area, a Desert Ecosystem of Northwestern China.

    PubMed

    He, Xuemin; Lv, Guanghui; Qin, Lu; Chang, Shunli; Yang, Min; Yang, Jianjun; Yang, Xiaodong

    2015-01-01

    One of the primary limiting factors for biological activities in desert ecosystems is nitrogen (N). This study therefore examined the effects of N and investigated the responses of an arid ecosystem to global change. We selected the typical desert plant Populus euphratica in a desert ecosystem in the Ebinur Lake area to evaluate the effects of N deposition on desert soil respiration. Three levels of N deposition (0, 37.5 and 112.5 kg·N·ha-1·yr-1) were randomly artificially provided to simulate natural N deposition. Changes in the soil respiration rates were measured from July to September in both 2010 and 2013, after N deposition in April 2010. The different levels of N deposition affected the total soil N, soil organic matter, soil C/N ratio, microorganism number, and microbial community structure and function. However, variable effects were observed over time in relation to changes in the magnitude of N deposition. Simulated high N deposition significantly reduced the soil respiration rate by approximately 23.6±2.5% (P<0.05), whereas low N deposition significantly increased the soil respiration rate by approximately 66.7±2.7% (P<0.05). These differences were clearer in the final growth stage (September). The different levels of N deposition had little effect on soil moisture, whereas N deposition significantly increased the soil temperature in the 0-5 cm layer (P<0.05). These results suggest that in the desert ecosystem of the Ebinur Lake area, N deposition indirectly changes the soil respiration rate by altering soil properties. PMID:26379186

  12. Effects of Simulated Nitrogen Deposition on Soil Respiration in a Populus euphratica Community in the Ebinur Lake Area, a Desert Ecosystem of Northwestern China

    PubMed Central

    He, Xuemin; Lv, Guanghui; Qin, Lu; Chang, Shunli; Yang, Min; Yang, Jianjun; Yang, Xiaodong

    2015-01-01

    One of the primary limiting factors for biological activities in desert ecosystems is nitrogen (N). This study therefore examined the effects of N and investigated the responses of an arid ecosystem to global change. We selected the typical desert plant Populus euphratica in a desert ecosystem in the Ebinur Lake area to evaluate the effects of N deposition on desert soil respiration. Three levels of N deposition (0, 37.5 and 112.5 kg·N·ha-1·yr-1) were randomly artificially provided to simulate natural N deposition. Changes in the soil respiration rates were measured from July to September in both 2010 and 2013, after N deposition in April 2010. The different levels of N deposition affected the total soil N, soil organic matter, soil C/N ratio, microorganism number, and microbial community structure and function. However, variable effects were observed over time in relation to changes in the magnitude of N deposition. Simulated high N deposition significantly reduced the soil respiration rate by approximately 23.6±2.5% (P<0.05), whereas low N deposition significantly increased the soil respiration rate by approximately 66.7±2.7% (P<0.05). These differences were clearer in the final growth stage (September). The different levels of N deposition had little effect on soil moisture, whereas N deposition significantly increased the soil temperature in the 0–5 cm layer (P<0.05). These results suggest that in the desert ecosystem of the Ebinur Lake area, N deposition indirectly changes the soil respiration rate by altering soil properties. PMID:26379186

  13. Effects of grazing on photosynthetic features and soil respiration of rangelands in the Tianshan Mountains of Northwest China

    PubMed Central

    Liu, Hua; Zang, Runguo; Chen, Han Y. H.

    2016-01-01

    Rangelands play a critical role in the global carbon cycle. However, the eco-physiological mechanisms associated with the effects of grazing on leaf photosynthesis and soil respiration remain poorly understood. To examine the impacts of grazing on leaf photosynthesis and soil respiration, we measured the photosynthetic parameters of the dominant species (Trifolium repens) and the soil respiration in grazed and ungrazed rangelands in the Tianshan Mountains of China. We found that grazing reduced the daily maximum net photosynthetic rate and soil respiration rates by 35% and 15%, respectively. The photosynthetic quantum yield, dark respiratory rate, and water use efficiency of T. repens leaves were reduced in grazed plots by 33.3%, 69.2%, and 21.5%, respectively. Our results demonstrated that grazing reduced carbon assimilation while increasing soil respiration within the rangelands in the Tianshan Mountains. PMID:27452980

  14. Effects of grazing on photosynthetic features and soil respiration of rangelands in the Tianshan Mountains of Northwest China.

    PubMed

    Liu, Hua; Zang, Runguo; Chen, Han Y H

    2016-01-01

    Rangelands play a critical role in the global carbon cycle. However, the eco-physiological mechanisms associated with the effects of grazing on leaf photosynthesis and soil respiration remain poorly understood. To examine the impacts of grazing on leaf photosynthesis and soil respiration, we measured the photosynthetic parameters of the dominant species (Trifolium repens) and the soil respiration in grazed and ungrazed rangelands in the Tianshan Mountains of China. We found that grazing reduced the daily maximum net photosynthetic rate and soil respiration rates by 35% and 15%, respectively. The photosynthetic quantum yield, dark respiratory rate, and water use efficiency of T. repens leaves were reduced in grazed plots by 33.3%, 69.2%, and 21.5%, respectively. Our results demonstrated that grazing reduced carbon assimilation while increasing soil respiration within the rangelands in the Tianshan Mountains. PMID:27452980

  15. Effects of grazing on photosynthetic features and soil respiration of rangelands in the Tianshan Mountains of Northwest China

    NASA Astrophysics Data System (ADS)

    Liu, Hua; Zang, Runguo; Chen, Han Y. H.

    2016-07-01

    Rangelands play a critical role in the global carbon cycle. However, the eco-physiological mechanisms associated with the effects of grazing on leaf photosynthesis and soil respiration remain poorly understood. To examine the impacts of grazing on leaf photosynthesis and soil respiration, we measured the photosynthetic parameters of the dominant species (Trifolium repens) and the soil respiration in grazed and ungrazed rangelands in the Tianshan Mountains of China. We found that grazing reduced the daily maximum net photosynthetic rate and soil respiration rates by 35% and 15%, respectively. The photosynthetic quantum yield, dark respiratory rate, and water use efficiency of T. repens leaves were reduced in grazed plots by 33.3%, 69.2%, and 21.5%, respectively. Our results demonstrated that grazing reduced carbon assimilation while increasing soil respiration within the rangelands in the Tianshan Mountains.

  16. [Soil heterotrophic respiration and its sensitivity to soil temperature and moisture in Liquidambar formosana and Pinus massoniana forests in hilly areas of southeast Hubei Province, China].

    PubMed

    Wang, Chuan-hua; Chen, Fang-qing; Wang, Yuan; Li, Jun-qing

    2011-03-01

    Field monitoring was conducted to study the annual dynamics of soil heterotrophic respiration and soil temperature and moisture in Liquidambar formosana and Pinus massoniana forests in hilly areas of southeast Hubei Province, China. At the same time, laboratory experiment was performed to study the heterotrophic respiration rate along soil profile, and the sensitivity of surface soil (0-5 cm) heterotrophic respiration to soil temperature and moisture. Then, a model was established to valuate the potential effects of warming change on the soil heterotrophic respiration in study area. In L. formosana and P. massoniana forests, the soil heterotrophic respiration rate in 0-5 cm layer was 2.39 and 2.62 times, and 2.01 and 2.94 times of that in 5-10 cm and 10-20 cm layers, respectively, illustrating that soil heterotrophic respiration mainly occurred in 0-5 cm surface layer. The temperature sensitivity factor (Q10) of soil heterotrophic respiration in 0-5 cm, 5-10 cm, and 10-20 cm layers was 2.10, 1.86, and 1.78 in L. formosana forest, and 1.86, 1.77, and 1.44 in P. massoniana forest, respectively. The relationship between surface soil heterotrophic respiration and temperature (T) well fitted exponential function R = alphaexp (beta3T), and that between surface soil heterotrophic respiration and moisture (W) well fitted quadratic function R = a+bW+cW2. Therefore, the relationship of surface soil heterotrophic respiration with soil temperature and moisture could be described by the model lnR = a+bW+cW2 +dT+eT2, which suggested that the response of soil heterotrophic respiration to soil moisture was depended on soil temperature, i.e., the sensitivity decreased with decreasing soil temperature. The calculation of the annual soil heterotrophic respiration rate in the two forests with the established model showed that the calculated respiration rate was a slightly higher in L. formosana forest but close to the measured one in P. massoniana forest, illustrating the applied

  17. Effects of aging herbicide mixtures on soil respiration and plant survival in soils from a pesticide-contaminated site

    SciTech Connect

    Kruger, E.L.; Anhalt, J.C.; Anderson, T.A.

    1996-10-01

    Three herbicides, atrazine, metolachlor, and pendimethalin, were applied individually and in all possible combinations to soil taken from a pesticide-contaminated site in Iowa. The rate of application for each chemical was 50 {mu}g/g, representative of contamination problems at mixing and loading areas of agrochemical dealer sites. Treated soils were incubated at 24{degrees}C in the dark for 0, 21, and 63 d, and soil moisture tension was maintained at -33 kPa. Soil respiration was measured daily by using an infrared gas analyzer for 10 d at the end of each incubation period. Subsamples of treated soils were used in plant germination and survival studies. Concentrations of each herbicide were determined by gas chromatography at day 0, 21, and 63. Soil respiration was elevated for the first 6 d immediately following treatment, and then declined to very low levels. At the end of day 21 and 63, soil respiration remained at very low levels. The half-lives for atrazine, metolachlor, and pendimethalin individually in soil or in combination with one and/or the other herbicide will be reported. The results of germination and survival studies with kochia, giant foxtail, birdsfoot trefoil, crown vetch, and soybean will also be reported.

  18. Long-term half-hourly measurement of soil CO2 concentration and soil respiration in a temperate deciduous forest

    NASA Astrophysics Data System (ADS)

    Hirano, Takashi; Kim, Honghyun; Tanaka, Yumiko

    2003-10-01

    We conducted a field experiment in a cool-temperate deciduous forest to investigate the dynamic behavior of soil CO2 and the vertical distribution of soil respiration. Soil CO2 concentration (C) was measured half-hourly at four depths for 6 months in 2000 with infrared gas analyzers installed below ground. Using C profiles, soil surface CO2 efflux (F0), CO2 production rates of the topsoil (PA), and CO2 flux from the subsoil to topsoil (FCA) were evaluated half-hourly by applying Fick's first law. Some remarkable short-term and long-term variations were found in C, F0, PA, FCA, and the contribution of topsoil respiration to total soil respiration (PA/F0), which include (1) rapid increase in C and decrease in F0 and PA due to rainwater infiltration, (2) diurnal variation in C coupled with that of the atmosphere, (3) diurnal variation in F0 and PA similar to that of topsoil temperature, (4) decrease in C, F0, and PA following soil drying in August, (5) linearly increasing FCA between late May and mid-September, and (6) decrease in PA/F0 from around 0.9 during summer to 0.3 in November. The variation of PA was mainly controlled by soil temperature at -0.07 m between 7° and 17°C, although PA did not respond well to soil temperature above and below this temperature range. Above 17°C, PA increased linearly with soil moisture, and moisture variation accounted for the PA decrease in August. Neither temperature nor moisture explained the PA behavior below 7°C. Subsoil respiration (FCA) showed an exponential relationship with soil temperature at -1 m.

  19. Responses of soil respiration to elevated CO2, air warming, and changing soil water availability in an old-field grassland

    SciTech Connect

    Wan, Shiqiang; Norby, Richard J; Childs, Joanne; Weltzin, Jake

    2007-01-01

    Responses of soil respiration to atmospheric and climatic change will have profound impacts on ecosystem and global C cycling in the future. This study was conducted to examine effects on soil respiration of the concurrent driving factors of elevated atmospheric CO2 concentration, rising temperature, and changing precipitation in a constructed old-field grassland in eastern Tennessee, USA. Model ecosystems of seven old-field species in 12 open-top chambers (4 m in diameter) were treated with two CO2 (ambient and ambient plus 300 ppm) and two temperature (ambient and ambient plus 3 C) levels. Two split plots with each chamber were assigned with high and low soil moisture levels. During the 19-month experimental period from June 2003 to December 2004, higher CO2 concentration and soil water availability significantly increased mean soil respiration by 35.8% and 15.7%, respectively. The effects of air warming on soil respiration varied seasonally from small reductions to significant increases to no response, and there was no significant main effect. In the wet side of elevated CO2 chambers, air warming consistently caused increases in soil respiration, whereas in other three combinations of CO2 and water treatments, warming tended to decrease soil respiration over the growing season but increase it over the winter. There were no interactive effects on soil respiration among any two or three treatment factors irrespective of testing time period. Temperature sensitivity of soil respiration was reduced by air warming, lower in the wet than the dry side, and not affected by CO2 treatment. Variations of soil respiration responses with soil temperature and soil moisture ranges could be primarily attributable to the seasonal dynamics of plant growth and its responses to the three treatments. Using a conceptual model to interpret the significant relationships of treatment-induced changes in soil respiration with changes in soil temperature and moisture observed in this study

  20. A Vineyard Agroecosystem: Disturbance and Precipitation Affect Soil Respiration under Mediterranean Conditions

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We investigated impacts of agricultural management practices on soil respiration (Rs) in a Cabernet sauvignon (Vitis vinifera) vineyard (Oakville, CA; November 2003 – December 2005). We determined 1) Rs’s response to cover cropping, mowing and tillage, 2) environmental drivers of Rs and 3) total ann...

  1. METHODOLOGY FOR ASSESSING RESPIRATION AND CELLULAR INCORPORATION OF RADIOLABELED SUBSTRATES BY SOIL MICROBIAL COMMUNITIES (JOURNAL VERSION)

    EPA Science Inventory

    A method is described for determining biodegradation kinetics of both naturally occurring and xenobiotic compounds in surface and subsurface soil samples. The method measures both respiration and uptake into cellular biomass of 14C-labeled substrates. After separation of the cell...

  2. Changes in soil respiration across a chronosequence of tallgrass prairie reconstructions

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Close relationships among climatic factors and soil respiration (Rs) are commonly reported. However, variation in Rs across the landscape is compounded by site-specific differences that impede the development of spatially explicit models and whose causes are not clearly known. Among factors that inf...

  3. Remote sensing-based estimation of annual soil respiration at two contrasting forest sites

    DOE PAGESBeta

    Gu, Lianhong; Huang, Ni; Black, T. Andrew; Wang, Li; Niu, Zheng

    2015-11-23

    Soil respiration (Rs), an important component of the global carbon cycle, can be estimated using remotely sensed data, but the accuracy of this technique has not been thoroughly investigated. In this article, we proposed a methodology for the remote estimation of annual Rs at two contrasting FLUXNET forest sites (a deciduous broadleaf forest and an evergreen needleleaf forest).

  4. Old soil carbon losses increase with ecosystem respiration in experimentally thawed tundra

    NASA Astrophysics Data System (ADS)

    Hicks Pries, Caitlin E.; Schuur, Edward A. G.; Natali, Susan M.; Crummer, K. Grace

    2016-02-01

    Old soil carbon (C) respired to the atmosphere as a result of permafrost thaw has the potential to become a large positive feedback to climate change. As permafrost thaws, quantifying old soil contributions to ecosystem respiration (Reco) and understanding how these contributions change with warming is necessary to estimate the size of this positive feedback. We used naturally occurring C isotopes (δ13C and Δ14C) to partition Reco into plant, young soil and old soil sources in a subarctic air and soil warming experiment over three years. We found that old soil contributions to Reco increased with soil temperature and Reco flux. However, the increase in the soil warming treatment was smaller than expected because experimentally warming the soils increased plant contributions to Reco by 30%. On the basis of these data, an increase in mean annual temperature from -5 to 0 °C will increase old soil C losses from moist acidic tundra by 35-55 g C m-2 during the growing season. The largest losses will probably occur where the plant response to warming is minimal.

  5. The effect of young biochar on soil respiration

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The low temperature pyrolysis of organic material produces biochar, a charcoal like substance. Biochar is being promoted as a soil amendment to enhance soil quality, it is also seen as a mechanism of long-term sequestration of carbon. Our experiments tested the hypothesis that biochar is inert in so...

  6. The effect of young biochar on soil respiration

    SciTech Connect

    Smith, Jeffery L.; Collins, Harold P.; Bailey, Vanessa L.

    2010-12-01

    The low temperature pyrolysis of organic material produces biochar, a charcoal like substance. Biochar is being promoted as a soil amendment to enhance soil quality, it is also seen as a mechanism of lomg-term sequestration of carbon. Our experiments tested the hypothesis that biochar is inert in soil. However, we measured an increase in CO2 production from soils after biochar amendment which increased with increasing rates of biochar. The ∂13C signature of the CO2 evolved in the first several days of the incubation was the same as the ∂13C signature of the biochar, confirming that biochar contributed to the CO2 flux. This effect diminished by day 6 of the incubation suggesting that most of the biochar C is slowly decomposing. Thus, aside from this short term mineralization increasing soil C with biochar may indeed be a long term C storage mechanism.

  7. Characteristics and origin of organic matter and basal respiration of soils from Majella massif (Central Apennines, Italy)

    NASA Astrophysics Data System (ADS)

    Basili, M.; Cioci, C.; Cocco, S.; Agnelli, A.; di Peco, D.; Ferraris, P.; Corti, G.

    2009-04-01

    The effects of the global climate change on the soil organic matter (SOM) are still open to debate. Many studies hypothesize an increase of the CO2 fluxes from the soil following the rise of air temperature, especially for the high latitude soils where the low temperatures have a protective effect on the SOM, holding the mineralization reactions back. We studied the feedback between soil and climate change in the Mediterranean environments, on patterned ground soils and soils developed from glacial lacustrine sediments found in the high-elevated areas (2500 m a.s.l.) of Majella massif (Central Apennines, Italy). Here, several profiles were opened and the soil described and sampled according to the recognized horizons. The samples were characterised according to the routine analyses and the SOM extracted according to the International Humic Substances Society protocol. The obtained humic and fulvic acids were characterised for elemental composition and by Fourier-transform infrared (FT-IR) spectroscopy. Further, the basal respiration at 5°C, 20°C and 30°C for 20 days was determined on the samples collected from the superficial horizon of each soil. The extracted humic substances showed a particular composition, being mostly comprised of proteinaceous residues (amides II and III), polysaccarides, and esters and aliphatic compounds. This unusual chemical structure and the paucity of vegetation in the study area could support the hypothesis of a mainly soil animal origin of the SOM, probably due to residues of insects, arachnids and arthropods. In fact, the species belonging to these Orders are abundant in these ecosystems and, further, are often characterised by the presence of compounds, such as glycerine and glycoproteins, in their organic fluids that act as antifreezing systems. The basal respiration experiments indicated that the soil microbial community was active at 5°C, while at 20°C or 30°C rather no respiration occurred; further, after 20 days at both

  8. Effect of vegetation of transgenic Bt rice lines and their straw amendment on soil enzymes, respiration, functional diversity and community structure of soil microorganisms under field conditions.

    PubMed

    Fang, Hua; Dong, Bin; Yan, Hu; Tang, Feifan; Wang, Baichuan; Yu, Yunlong

    2012-01-01

    With the development of transgenic crops, there is an increasing concern about the possible adverse effects of their vegetation and residues on soil environmental quality. This study was carried out to evaluate the possible effects of the vegetation of transgenic Bt rice lines Huachi B6 (HC) and TT51 (TT) followed by the return of their straw to the soil on soil enzymes (catalase, urease, neutral phosphatase and invertase), anaerobic respiration activity, microbial utilization of carbon substrates and community structure, under field conditions. The results indicated that the vegetation of the two transgenic rice lines (HC and TT) and return of their straw had few adverse effects on soil enzymes and anaerobic respiration activity compared to their parent and distant parent, although some transient differences were observed. The vegetation and subsequent straw amendment of Bt rice HC and TT did not appear to have a harmful effect on the richness, evenness and community structure of soil microorganisms. No different pattern of impact due to plant species was found between HC and TT. It could be concluded that the vegetation of transgenic Bt rice lines and the return of their straw as organic fertilizer may not alter soil microbe-mediated functions. PMID:23513447

  9. Land-use and climate effects on soil respiration quantified with a landscape sensor network

    NASA Astrophysics Data System (ADS)

    Crum, S.; Jenerette, D.

    2014-12-01

    Land-use change alters the magnitudes and variability of soil respiration (Rs). However, the importance of ecosystem and landscape drivers of Rs remain poorly understood from an empirical and mechanistic standpoint and likely vary across climate gradients. To address this knowledge gap we asked, what regulates the spatial and temporal variation of Rs across a human dominated region? From a landscape perspective, climate and land-use are hypothesized to be key drivers of Rs. From a soil physiological perspective, variability in temperature, moisture, and substrate availability regulate Rs. According to an inverse metabolic activity hypothesis, systems with higher metabolic activity will have less temporal variability in Rs than those with lower rates. Alternatively, soil substrate availability may drive sensitivity of Rs to water inputs. To quantify variability in Rs and test hypotheses of its regulation we deployed an Rs sensor network beginning in November 2013 with nodes distributed across three land-use types - lawn, agriculture, and wildland - at three sub-regions spanning a coastal to inland to desert climate gradient (total 9 sites, 3 land-uses x 3 sub-regions). At each node we are measuring soil Rs using the flux gradient approach, which includes soil state CO2 sensors, temperature, and moisture measured at three depths and at five minute intervals. We analyzed the data for the winter sampling period, which is southern California's rainy season. The mean of Rs was lowest at the coastal and highest in the desert sub-regions for both lawn (3.99 and 4.7 μmol CO2 m-2 s-1) and wildland (0.23 and 0.49 μmol CO2 m-2 s-1) land-uses. Rs was the highest in the inland sub-region for agricultural land-uses (4.1 μmol CO2 m-2 s-1). Lawn and wildland land-uses had increasing coefficient of variation in Rs across the coastal to desert climate gradient, while agriculture had decreasing variation. Sites that had higher mean fluxes and soil organic matter, a proxy for

  10. Soil Respiration Measurements in a Temperate and a Tropical Forest

    NASA Astrophysics Data System (ADS)

    Pitz, S. L.; Szlavecz, K. A.; Xia, L.; Chen, Y.; Gupchup, J. A.

    2009-12-01

    The development of low power sensor technology has allowed for long term, continuous measurements of environmental conditions in remote locations. We have deployed a wireless sensor network to monitor soil temperature, moisture, and carbon dioxide (CO2) concentrations at the Smithsonian Environmental Research Center (SERC). A sister pilot system was deployed at an upland tropical rainforest in Yasuni National Park, Ecuador. Here we compare soil CO2 efflux data from the two systems at similar temperature conditions. At both sites, the chamber and gas well methods were used to quantify soil CO2 efflux. Average soil CO2 efflux measured by the chamber method was 2.3 umol m-2 s-1 ± 0.8 and 2.8 umol m-2 s-1± 0.4 at SERC and Yasuni respectively. The Millington Quirk and the Penman models were used to calculate the efflux. Average effluxes generated by the Millington Quick gas well method were 3.6 umol m-2 s-1 ± 0.2 and 5.6 umol m-2 s-1± 0.4 at SERC and Yasuni, respectively. Soil temperatures varied from 20-23 °C during the time periods that were compared. Concentrations were higher and the variation was larger at shallow depths in the rainforest soil (between 2300 ppm and 5000 ppm versus 2000 ppm to 3000 ppm) than the temperature forest soil at weather conditions. Later, under different temperature and moisture regimes the CO2 concentrations at the shallow depth at SERC have reached values over 20,000 ppm. Rain events were captured in both ecosystems with a sharper pulse at SERC. At both sites soil CO2 efflux correlated with soil moisture strongly than with temperature over short time periods. Soil CO2 effluxes were calculated to be three times higher when using the Penman model versus the Millington Quirk model. Also, the latter model was more comparable to the chamber measurements but the values were still 30-40% greater. Gas well models need to be refined to more accurately reflect the actual efflux and need to incorporate potential for aqueous CO2 transport in

  11. Response of heterotrophic soil respiration to changes in moisture: what do data and theory tell us?

    NASA Astrophysics Data System (ADS)

    Moyano, Fernando; Manzoni, Stefano; Chenu, Claire

    2013-04-01

    Soil moisture strongly affects the dynamics of soil organic matter­­ and is central to predict changes in soil carbon stocks from site to global scales. Despite its importance in controlling soil carbon transformations, the mechanisms involved are still poorly represented in models, mostly as highly simplified empirical relationships. To improve such representations we approached the problem in two ways: First, a synthesis analysis of laboratory data was performed to explore the variability of moisture effects on heterotrophic respiration across soil types. Second, we used theory and established relationships to build a semi-mechanistic model that predicts the response of soil heterotrophic respiration to changes in moisture and its dependence on soil properties. With the first approach, statistical models of the response of soil heterotrophic respiration to moisture were obtained. The inclusion of soil properties (clay, bulk density and organic matter) as predictor variables improved the agreement between model results and observations. These models are useful to visualize the change in the response across different soil types. They thus improve over other commonly used empirical relationships, but because they remain a statistical approximation based on linear regressions they are potentially biased and could lead to systematic errors in predictions. In the second approach we explored the theory linking gas diffusivity and heterotrophic respiration in soils, as well as the effect of soil clay content, pore space, organic matter and temperature. The advantage of a mechanistically based model is that it can be modified or expanded to test different theories or processes, and extrapolation of predictor variables will not usually lead to unrealistic predictions. Observations and model predictions from the two approaches are shown to agree in many points, e.g. in the influence of soil clay content. But both the empirical and the more mechanistic model are unable to

  12. What Lies Within: Contributions of the Organic Horizon to Forest Soil Respiration

    NASA Astrophysics Data System (ADS)

    Julia, P.; Phillips, C. L.; Bond, B. J.

    2008-12-01

    Soil respiration rates can exhibit tremendous spatial variability, making it difficult to ascertain the proximal causes of CO2 production. In coniferous forest soils, a large proportion of CO2 is produced in the organic layer, and better characterizing the organisms and chemical composition of this heterogeneous horizon may contribute to a predictive understanding of soil respiration rates. In this study we sought to 1) identify characteristics of organic soil that could serve as predictors of soil respiration rates, including the presence/absence of fungal mats, and 2) determine the proportion of total surface efflux derived from the organic horizon seasonally. Working in an old-growth Douglas-fir stand in the Central Oregon Cascades (HJ Andrews LTER), we found that fungal mats of the Piloderma genus colonized over 56% of the forest floor, and organic horizons containing these mats had on average 10% higher surface efflux rates than neighboring non-mat soils. In addition to containing fungal mats, we found the organic horizon contained significantly more fine root biomass than the top 10cm of mineral soil. By measuring moisture and CO2 concentrations throughout mat and non-mat soil profiles, we evaluated CO2 production within soil horizons using the principles of Fick's First Law and the diffusion gradient method. We found that the organic layers produced roughly half of the net CO2 flux and that higher surface efflux rates are associated with higher percent contributions of production in the organic layer. We also examined correlations between respiration rate and a suite of soil biological, physical, and chemical characteristics. While none of these factors correlated directly with surface efflux rates, we found litter depth to be an important variable that correlated with soil water content and pH, and we found that mat soils tended to have deeper litter than non-mat soils. This work indicates that in a mature coniferous forest, the organic horizon serves as a

  13. Development of an NDIR CO2 Sensor-Based System for Assessing Soil Toxicity Using Substrate-Induced Respiration

    PubMed Central

    Kaur, Jasmeen; Adamchuk, Viacheslav I.; Whalen, Joann K.; Ismail, Ashraf A.

    2015-01-01

    The eco-toxicological indicators used to evaluate soil quality complement the physico-chemical criteria employed in contaminated site remediation, but their cost, time, sophisticated analytical methods and in-situ inapplicability pose a major challenge to rapidly detect and map the extent of soil contamination. This paper describes a sensor-based approach for measuring potential (substrate-induced) microbial respiration in diesel-contaminated and non-contaminated soil and hence, indirectly evaluates their microbial activity. A simple CO2 sensing system was developed using an inexpensive non-dispersive infrared (NDIR) CO2 sensor and was successfully deployed to differentiate the control and diesel-contaminated soils in terms of CO2 emission after glucose addition. Also, the sensor system distinguished glucose-induced CO2 emission from sterile and control soil samples (p ≤ 0.0001). Significant effects of diesel contamination (p ≤ 0.0001) and soil type (p ≤ 0.0001) on glucose-induced CO2 emission were also found. The developed sensing system can provide in-situ evaluation of soil microbial activity, an indicator of soil quality. The system can be a promising tool for the initial screening of contaminated environmental sites to create high spatial density maps at a relatively low cost. PMID:25730479

  14. Development of an NDIR CO₂ sensor-based system for assessing soil toxicity using substrate-induced respiration.

    PubMed

    Kaur, Jasmeen; Adamchuk, Viacheslav I; Whalen, Joann K; Ismail, Ashraf A

    2015-01-01

    The eco-toxicological indicators used to evaluate soil quality complement the physico-chemical criteria employed in contaminated site remediation, but their cost, time, sophisticated analytical methods and in-situ inapplicability pose a major challenge to rapidly detect and map the extent of soil contamination. This paper describes a sensor-based approach for measuring potential (substrate-induced) microbial respiration in diesel-contaminated and non-contaminated soil and hence, indirectly evaluates their microbial activity. A simple CO2 sensing system was developed using an inexpensive non-dispersive infrared (NDIR) CO2 sensor and was successfully deployed to differentiate the control and diesel-contaminated soils in terms of CO2 emission after glucose addition. Also, the sensor system distinguished glucose-induced CO2 emission from sterile and control soil samples (p ≤ 0.0001). Significant effects of diesel contamination (p ≤ 0.0001) and soil type (p ≤ 0.0001) on glucose-induced CO2 emission were also found. The developed sensing system can provide in-situ evaluation of soil microbial activity, an indicator of soil quality. The system can be a promising tool for the initial screening of contaminated environmental sites to create high spatial density maps at a relatively low cost. PMID:25730479

  15. The Temperature Optima and Temperature Sensitivity of Soil Respiration Explained By Macromolecular Rate Theory (MMRT).

    NASA Astrophysics Data System (ADS)

    Schipper, L. A.; O'Neill, T.; Arcus, V. L.

    2014-12-01

    One of the most fundamental factors controlling all biological and chemical processes is changing temperature. Temperature dependence was originally described by the Arrhenius function in the 19th century. This function provides an excellent description of chemical reaction rates. However, the Arrhenius function does not predict the temperature optimum of biological rates that is clearly evident in laboratory and field measurements. Previously, the temperature optimum of biological processes has been ascribed to denaturation of enzymes but the observed temperature optima in soil are often rather modest, occurring at about 40-50°C and generally less than recognised temperatures for protein unfolding. We have modified the Arrhenius function incorporating a temperature-dependent activation energy derived directly from first principles from thermodynamics of macromolecules. MacroMolecular Rate Theory (MMRT) accounts for large changes in the flexibility of enzymes during catalysis that result in changes in heat capacity (∆C‡p) of the enzyme during the reaction. MMRT predicts an initially Arrhenius-like response followed by a temperature optimum without the need for enzyme denaturation (Hobbs et al., 2013. ACS Chemical Biology. 8: 2388-2393). Denaturation, of course, occurs at much higher temperatures. We have shown that MMRT fits biogeochemical data collected from laboratory and field studies with important implications for changes in absolute temperature sensitivity as temperature rises (Schipper et al., 2014. Global Change Biology). As the temperature optimum is approached the absolute temperature sensitivity of biological processes decreases to zero. Consequently, the absolute temperature-sensitivity of soil biological processes depends on both the change in ecosystem temperature and the temperature optimum of the biological process. MMRT also very clearly explains why Q10 values decline with increasing temperature more quickly than would be predicted from the

  16. Influence of plant productivity over variability of soil respiration: a multi-scale approach

    NASA Astrophysics Data System (ADS)

    Curiel Yuste, J.

    2009-04-01

    To investigate the role of plant photosynthetic activity on the variations in soil respiration (SR), SR data obtained from manual sampling and automatic soil respiration chambers placed on eddy flux towers sites were used. Plant photosynthetic activity was represented as Gross Primary Production (GPP), calculated from the half hourly continuous measurements of Net Ecosystem Exchange (NEE). The role of plant photosynthetic activity over the variation in SR was investigated at different time-scales: data averaged hourly, daily and weekly were used to study the photosynthetic effect on SR dial variations (Hourly data), 15 days variations (Daily averages), monthly variations (daily and weekly averages) and seasonal variations (weekly data). Our results confirm the important role of plant photosynthetic activity on the variations of SR at each of the mentioned time-scales. The effect of photosynthetic activity on SR was high on hourly time-scale (dial variations of SR). At half of the studied ecosystems GPP was the best single predictor of dial variations of SR. However at most of the studied sites the combination of soil temperature and GPP was the best predictor of dial variations in SR. The effect of aboveground productivity over dial variations of SR lagged on the range of 5 to 15 hours, depending on the ecosystem. At daily to monthly time scale variations of SR were in general better explained with the combination of temperature and moisture variations. However, ‘jumps' in average weekly SR during the growing season yielded anomaly high values of Q10, in some cases above 1000, which probably reflects synoptic changes in photosynthates translocation from plant activity. Finally, although seasonal changes of SR were in general very well explained by temperature and soil moisture, seasonality of SR was better correlated to seasonality of GPP than to seasonality of soil temperature and/or soil moisture. Therefore the magnitude of the seasonal variation in SR was in

  17. Effects of manipulated above- and belowground organic matter input on soil respiration in a Chinese pine plantation.

    PubMed

    Fan, Juan; Wang, Jinsong; Zhao, Bo; Wu, Lianhai; Zhang, Chunyu; Zhao, Xiuhai; Gadow, Klaus V

    2015-01-01

    Alteration in the amount of soil organic matter input can have profound effect on carbon dynamics in forest soils. The objective of our research was to determine the response in soil respiration to above- and belowground organic matter manipulation in a Chinese pine (Pinus tabulaeformis) plantation. Five organic matter treatments were applied during a 2-year experiment: both litter removal and root trenching (LRRT), only litter removal (LR), control (CK), only root trenching (RT) and litter addition (LA). We found that either aboveground litter removal or root trenching decreased soil respiration. On average, soil respiration rate was significantly decreased in the LRRT treatment, by about 38.93% ± 2.01% compared to the control. Soil respiration rate in the LR treatment was 30.65% ± 1.87% and in the RT treatment 17.65% ± 1.95% lower than in the control. Litter addition significantly increased soil respiration rate by about 25.82% ± 2.44% compared to the control. Soil temperature and soil moisture were the main factors affecting seasonal variation in soil respiration. Up to the 59.7% to 82.9% seasonal variation in soil respiration is explained by integrating soil temperature and soil moisture within each of the various organic matter treatments. The temperature sensitivity parameter, Q10, was higher in the RT (2.72) and LA (3.19) treatments relative to the control (2.51), but lower in the LRRT (1.52) and LR treatments (1.36). Our data suggest that manipulation of soil organic matter input can not only alter soil CO2 efflux, but also have profound effect on the temperature sensitivity of organic carbon decomposition in a temperate pine forest. PMID:25970791

  18. Effects of Manipulated Above- and Belowground Organic Matter Input on Soil Respiration in a Chinese Pine Plantation

    PubMed Central

    Zhao, Bo; Wu, Lianhai; Zhang, Chunyu; Zhao, Xiuhai; Gadow, Klaus v.

    2015-01-01

    Alteration in the amount of soil organic matter input can have profound effect on carbon dynamics in forest soils. The objective of our research was to determine the response in soil respiration to above- and belowground organic matter manipulation in a Chinese pine (Pinus tabulaeformis) plantation. Five organic matter treatments were applied during a 2-year experiment: both litter removal and root trenching (LRRT), only litter removal (LR), control (CK), only root trenching (RT) and litter addition (LA). We found that either aboveground litter removal or root trenching decreased soil respiration. On average, soil respiration rate was significantly decreased in the LRRT treatment, by about 38.93% ± 2.01% compared to the control. Soil respiration rate in the LR treatment was 30.65% ± 1.87% and in the RT treatment 17.65% ± 1.95% lower than in the control. Litter addition significantly increased soil respiration rate by about 25.82% ± 2.44% compared to the control. Soil temperature and soil moisture were the main factors affecting seasonal variation in soil respiration. Up to the 59.7% to 82.9% seasonal variation in soil respiration is explained by integrating soil temperature and soil moisture within each of the various organic matter treatments. The temperature sensitivity parameter, Q10, was higher in the RT (2.72) and LA (3.19) treatments relative to the control (2.51), but lower in the LRRT (1.52) and LR treatments (1.36). Our data suggest that manipulation of soil organic matter input can not only alter soil CO2 efflux, but also have profound effect on the temperature sensitivity of organic carbon decomposition in a temperate pine forest. PMID:25970791

  19. Diel pattern of soil respiration in N-amended soil under maize cultivation

    NASA Astrophysics Data System (ADS)

    Ding, Weixin; Cai, Yan; Cai, Zucong; Zheng, Xunhua

    To understand maize- and N-induced diel variations in CO 2 emission, we examined hourly CO 2 emissions during the three typical growth stages of maize in sandy loam soil. There was a distinct diel pattern in soil CO 2 emissions, with the peak occurring between 14:00 and 18:00 and the trough occurring between 0:00 and 4:00. Maize presence delayed the time of the peak. The absolute amount and diel fluctuation of CO 2 emissions tended to diminish with time in the bare soil fertilized with 150 kg N ha -1 (BS). In contrast, N-fertilized maize (N150) significantly enhanced the total amount of CO 2 emissions and the peak-trough differences in CO 2 emissions, which reached a maximum at the pollination stage and then decreased. Control soil (CK) containing maize but no N fertilizer had highest overall CO 2 emissions but reduced diel fluctuation because rhizosphere respiration was elevated in the nighttime. Soil temperature accounted for 61-71% of diel variation in the BS treatment but for only 44-59% and 38-58% in the N150 and CK treatments, respectively. Photosynthesis rates affected diel variation at the seedling and pollination stages. Both temperature and photosynthesis rates together explained up to 67-84% of diel variation at the seedling and pollination stages in the N150 treatment, but only 61% at the seedling stage in the CK treatment due to more CO 2 released in the nighttime. The increased nighttime CO 2 release, in turn, decreased the effect of temperature and even reduced the influence of photosynthesis rate on diel variations in CO 2 release. Based on the present results, the best time for obtaining a representative daily CO 2 measurement was found to be approximately 8:00 at the seedling stage and 9:00-11:00 at the other growth stages. The current findings indicate that N addition reduces soil CO 2 emissions and its diel fluctuation.

  20. Soil moisture sensitivity of autotrophic and heterotrophic forest floor respiration in boreal xeric pine and mesic spruce forests

    NASA Astrophysics Data System (ADS)

    Ťupek, Boris; Launiainen, Samuli; Peltoniemi, Mikko; Heikkinen, Jukka; Lehtonen, Aleksi

    2016-04-01

    Litter decomposition rates of the most process based soil carbon models affected by environmental conditions are linked with soil heterotrophic CO2 emissions and serve for estimating soil carbon sequestration; thus due to the mass balance equation the variation in measured litter inputs and measured heterotrophic soil CO2 effluxes should indicate soil carbon stock changes, needed by soil carbon management for mitigation of anthropogenic CO2 emissions, if sensitivity functions of the applied model suit to the environmental conditions e.g. soil temperature and moisture. We evaluated the response forms of autotrophic and heterotrophic forest floor respiration to soil temperature and moisture in four boreal forest sites of the International Cooperative Programme on Assessment and Monitoring of Air Pollution Effects on Forests (ICP Forests) by a soil trenching experiment during year 2015 in southern Finland. As expected both autotrophic and heterotrophic forest floor respiration components were primarily controlled by soil temperature and exponential regression models generally explained more than 90% of the variance. Soil moisture regression models on average explained less than 10% of the variance and the response forms varied between Gaussian for the autotrophic forest floor respiration component and linear for the heterotrophic forest floor respiration component. Although the percentage of explained variance of soil heterotrophic respiration by the soil moisture was small, the observed reduction of CO2 emissions with higher moisture levels suggested that soil moisture response of soil carbon models not accounting for the reduction due to excessive moisture should be re-evaluated in order to estimate right levels of soil carbon stock changes. Our further study will include evaluation of process based soil carbon models by the annual heterotrophic respiration and soil carbon stocks.

  1. Effects of nitrogen fertilization on soil N2O emissions and soil respiration in temperate grassland in Inner Mongolia, China

    NASA Astrophysics Data System (ADS)

    Dong, Y.; Qi, Y.; Peng, Q.

    2012-04-01

    Nitrogen addition to soil can play a vital role in influencing nitrogen balance and the losses of soil carbon by respiration in N-deficient terrestrial ecosystems. The aim of this study was to clarify the effects of different levels of nitrogen fertilization (HN:200 kg N ha-1y-1, MN:100 kg N ha-1y-1 and LN:50 kg N ha-1y-1) on soil N2O emissions and soil respiration compared with non-fertilization(CK, 0 kg N ha-1y-1), from July 2007 to September 2008, in temperate grassland in Inner Mongolia, China. Several N fertilizer forms were included(CAN:calcium ammonium nitrate, AS:ammonium sulphate and NS:sodium nitrate) and a static closed chamber method was used as gas fluxes measurement. Our data showed that peak N2O fluxes induced by N treatments were concentrated in short periods (2 to 3 weeks) after fertilization in summer and in soil thawing periods in early spring; there were similarly low N2O fluxes from all treatments in the remaining seasons of the year. The three N levels increased annual N2O emissions significantly(P<0.05) in the order of MN>HN>LN compared with the CK(control) treatment in year 1; in year 2, the elevation of annual N2O emissions was significant (P<0.05) by HN and MN treatments but was insignificant by LN treatments (P>0.05). The three N forms also had strong effects on N2O emissions. Significantly (P<0.05) higher annual N2O emissions were observed in the soils of CAN and AS fertilizer treatments than in the soils of NS fertilizer treatments in both measured years, but the difference between CAN and AS was not significant (P>0.05). Annual N2O emission factors (EF) ranged from 0.060 to 0.298% for different N fertilizer treatments in the two observed years, with an overall EF value of 0.125%. The EF values were by far less than the mean default EF proposed by the Intergovernmental Panel on Climate Change(IPCC). Our results also showed that N fertilization did not change the seasonal patterns of soil respiration, which were mainly controlled by soil

  2. Basal respiration - a proxy to understand spatial variability of soil CO2 emissions in urban regions

    NASA Astrophysics Data System (ADS)

    Vasenev, Viacheslav; Stoorvogel, Jetse; Ananyeva, Nadezhda; Ivashchenko, Kristina; Vizirskaya, Marya; Valentini, Riccardo

    2015-04-01

    Soil respiration (Rs) is an important terrestrial CO2 efflux and received significant attention at different scale levels. However, the sampling density is limited and global Rs databases are biased towards natural ecosystems and towards north America and Europe. This limits our understanding of the spatial variability of Rs. The methodological constraints of direct Rs measurements in the field limit the number of observations. As an alternative approach to approximate the spatial variability of Rs, we used basal respiration (BR) as an indirect measurement. First, the direct Rs and indirect BR measurements were compared at a 10 km2 test area in Moscow city, which included adjacent forests, croplands and urban lawn plots. Rs was monitored by in situ chamber approach with an IR Li-820 gas analyzer at 50 points during the growing season (June-October 2013, 9 time repetitions per point). In the same area, 32 locations were sampled and BR was measured under controlled conditions. Rs was affected by anthropogenic disturbance with the highest values in urban lawns. BR was mainly controlled by soil organic carbon (SOC) with maximum rates in the forested area. Total variability reported by direct observations was 10% higher, than one for BR, although the spatial variability captured by both approaches was similar confirmed by significant correlation between variance coefficients (CV) of the values. This shows that BR is a relevant proxy to analyze the spatial variability of Rs. Subsequently, the sampling area was expanded to the Moscow region for which respiration was mapped using digital soil mapping techniques and BR as a proxy for Rs. Although the absolute levels of respiration remained uncertain, the spatial patterns of BR are likely to correspond well with Rs patterns. Land use largely determined the spatial heterogeneity of soil respiration. Most variation occurred in the urban areas. BR is a relevant and straightforward proxy to understand patterns of Rs especially

  3. Soil respiration and photosynthetic uptake of carbon dioxide by ground-cover plants in four ages of jack pine forest

    USGS Publications Warehouse

    Striegl, R.G.; Wickland, K.P.

    2001-01-01

    Soil carbon dioxide (CO2) emission (soil respiration), net CO2 exchange after photosynthetic uptake by ground-cover plants, and soil CO2 concentration versus depth below land surface were measured at four ages of jack pine (Pinus banksiana Lamb.) forest in central Saskatchewan. Soil respiration was smallest at a clear-cut site, largest in an 8-year-old stand, and decreased with stand age in 20-year-old and mature (60-75 years old) stands during May-September 1994 (12.1, 34.6, 31.5, and 24.9 mol C??m-2, respectively). Simulations of soil respiration at each stand based on continuously recorded soil temperature were within one standard deviation of measured flux for 48 of 52 measurement periods, but were 10%-30% less than linear interpolations of measured flux for the season. This was probably due to decreased soil respiration at night modeled by the temperature-flux relationships, but not documented by daytime chamber measurements. CO2 uptake by ground-cover plants ranged from 0 at the clear-cut site to 29, 25, and 9% of total growing season soil respiration at the 8-year, 20-year, and mature stands. CO2 concentrations were as great as 7150 ppmv in the upper 1 m of unsaturated zone and were proportional to measured soil respiration.

  4. Rapid rebound of soil respiration following partial stand disturbance by tree girdling in a temperate deciduous forest.

    PubMed

    Levy-Varon, Jennifer H; Schuster, William S F; Griffin, Kevin L

    2014-04-01

    Forests serve an essential role in climate change mitigation by removing CO2 from the atmosphere. Within a forest, disturbance events can greatly impact C cycling and subsequently influence the exchange of CO2 between forests and the atmosphere. This connection makes understanding the forest C cycle response to disturbance imperative for climate change research. The goal of this study was to examine the temporal response of soil respiration after differing levels of stand disturbance for 3 years at the Black Rock Forest (southeastern NY, USA; oaks comprise 67% of the stand). Tree girdling was used to mimic pathogen attack and create the following treatments: control, girdling all non-oaks (NOG), girdling half of the oak trees (O50), and girdling all the oaks (OG). Soil respiratory rates on OG plots declined for 2 years following girdling before attaining a full rebound of belowground activity in the third year. Soil respiration on NOG and O50 were statistically similar to the control for the duration of the study although a trend for a stronger decline in respiration on O50 relative to NOG occurred in the first 2 years. Respiratory responses among the various treatments were not proportional to the degree of disturbance and varied over time. The short-lived respiratory response on O50 and OG suggests that belowground activity is resilient to disturbance; however, sources of the recovered respiratory flux on these plots are likely different than they were pre-treatment. The differential taxon response between oaks and non-oaks suggests that after a defoliation or girdling event, the temporal response of the soil respiratory flux may be related to the C allocation pattern of the affected plant group. PMID:24337785

  5. MEASUREMENT OF SOIL RESPIRATION IN SITU: CHAMBER TECHNIQUES

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Chambers temporarily sealed to the soil surface are important and often the only means of measuring trace gas emissions to the atmosphere. However, such chamber measurements are not exempt from methodological problems. This review article identifies known sources of chamber-induced errors encounte...

  6. Soil respiration patterns and controls in limestone cedar glades

    USGS Publications Warehouse

    Cartwright, Jennifer M.; Hui, Dafeng

    2015-01-01

    Soil depth, SOM, and vegetation cover were important drivers of Rs in limestone cedar glades. Seasonal Rs patterns reflected those for mesic temperate grasslands more than for semi-arid ecosystems, in that Rs primarily tracked temperature for most of the year.

  7. Effects of land-use change and fungicide application on soil respiration in playa wetlands and adjacent uplands of the U.S. High Plains.

    PubMed

    Daniel, Dale W; Smith, Loren M; Belden, Jason B; McMurry, Scott T; Swain, Shella

    2015-05-01

    With the increased use of fungicides in cultivated regions such as the southern High Plains (SHP), U.S., unintentional runoff and drift as well as direct overspray during aerial application lead to environmental exposures that may influence soil microbial communities and related biogeochemical functioning. Our goal was to examine the effects of two popular fungicides Headline (pyraclostrobin) and Quilt (azoxystrobin/propiconazole) on respiration from soil microbial communities in playa wetlands embedded in cropland and native grassland and their adjacent watersheds. We monitored fungicide effects (at levels of 0, .1×, 1× and 10× the label rate) by measuring respiration from plant matter amended soils collected from 6 cropland and 6 grassland playas and uplands. In addition, differences in microbial community structure among land use types were determined by measuring ergosterol levels in cropland and native grassland playas and uplands. Native grassland playas and their associated watersheds had up to 43% higher soil respiration rates than cropland playas and watersheds, indicating higher soil microbial activity. Application of either fungicide had no effect on soil respiration at any concentration in either land use type or habitat type (playa/watershed). Native grassland playas and watersheds had 3 and 1.6 times higher ergosterol content than cropland playas and watersheds. The lack of soil respiration response to fungicide application does not necessarily suggest that fungicides used in this study do not affect non-target soil microbial communities due to potential compensation by other biota. Future studies should further elucidate existing microorganism communities in playas and their watersheds. PMID:25668281

  8. Nitrogen deposition may enhance soil carbon storage via change of soil respiration dynamic during a spring freeze-thaw cycle period.

    PubMed

    Yan, Guoyong; Xing, Yajuan; Xu, Lijian; Wang, Jianyu; Meng, Wei; Wang, Qinggui; Yu, Jinghua; Zhang, Zhi; Wang, Zhidong; Jiang, Siling; Liu, Boqi; Han, Shijie

    2016-01-01

    As crucial terrestrial ecosystems, temperate forests play an important role in global soil carbon dioxide flux, and this process can be sensitive to atmospheric nitrogen deposition. It is often reported that the nitrogen addition induces a change in soil carbon dioxide emission in growing season. However, the important effects of interactions between nitrogen deposition and the freeze-thaw-cycle have never been investigated. Here we show nitrogen deposition delays spikes of soil respiration and weaken soil respiration. We found the nitrogen addition, time and nitrogen addition×time exerted the negative impact on the soil respiration of spring freeze-thaw periods due to delay of spikes and inhibition of soil respiration (p < 0.001). The values of soil respiration were decreased by 6% (low-nitrogen), 39% (medium-nitrogen) and 36% (high-nitrogen) compared with the control. And the decrease values of soil respiration under medium- and high-nitrogen treatments during spring freeze-thaw-cycle period in temperate forest would be approximately equivalent to 1% of global annual C emissions. Therefore, we show interactions between nitrogen deposition and freeze-thaw-cycle in temperate forest ecosystems are important to predict global carbon emissions and sequestrations. We anticipate our finding to be a starting point for more sophisticated prediction of soil respirations in temperate forests ecosystems. PMID:27358164

  9. Nitrogen deposition may enhance soil carbon storage via change of soil respiration dynamic during a spring freeze-thaw cycle period

    PubMed Central

    Yan, Guoyong; Xing, Yajuan; Xu, Lijian; Wang, Jianyu; Meng, Wei; Wang, Qinggui; Yu, Jinghua; Zhang, Zhi; Wang, Zhidong; Jiang, Siling; Liu, Boqi; Han, Shijie

    2016-01-01

    As crucial terrestrial ecosystems, temperate forests play an important role in global soil carbon dioxide flux, and this process can be sensitive to atmospheric nitrogen deposition. It is often reported that the nitrogen addition induces a change in soil carbon dioxide emission in growing season. However, the important effects of interactions between nitrogen deposition and the freeze-thaw-cycle have never been investigated. Here we show nitrogen deposition delays spikes of soil respiration and weaken soil respiration. We found the nitrogen addition, time and nitrogen addition×time exerted the negative impact on the soil respiration of spring freeze-thaw periods due to delay of spikes and inhibition of soil respiration (p < 0.001). The values of soil respiration were decreased by 6% (low-nitrogen), 39% (medium-nitrogen) and 36% (high-nitrogen) compared with the control. And the decrease values of soil respiration under medium- and high-nitrogen treatments during spring freeze-thaw-cycle period in temperate forest would be approximately equivalent to 1% of global annual C emissions. Therefore, we show interactions between nitrogen deposition and freeze-thaw-cycle in temperate forest ecosystems are important to predict global carbon emissions and sequestrations. We anticipate our finding to be a starting point for more sophisticated prediction of soil respirations in temperate forests ecosystems. PMID:27358164

  10. Water Organic Pollution and Eutrophication Influence Soil Microbial Processes, Increasing Soil Respiration of Estuarine Wetlands: Site Study in Jiuduansha Wetland

    PubMed Central

    Zhang, Yue; Wang, Lei; Hu, Yu; Xi, Xuefei; Tang, Yushu; Chen, Jinhai; Fu, Xiaohua; Sun, Ying

    2015-01-01

    Undisturbed natural wetlands are important carbon sinks due to their low soil respiration. When compared with inland alpine wetlands, estuarine wetlands in densely populated areas are subjected to great pressure associated with environmental pollution. However, the effects of water pollution and eutrophication on soil respiration of estuarine and their mechanism have still not been thoroughly investigated. In this study, two representative zones of a tidal wetland located in the upstream and downstream were investigated to determine the effects of water organic pollution and eutrophication on soil respiration of estuarine wetlands and its mechanism. The results showed that eutrophication, which is a result of there being an excess of nutrients including nitrogen and phosphorus, and organic pollutants in the water near Shang shoal located upstream were higher than in downstream Xia shoal. Due to the absorption and interception function of shoals, there to be more nitrogen, phosphorus and organic matter in Shang shoal soil than in Xia shoal. Abundant nitrogen, phosphorus and organic carbon input to soil of Shang shoal promoted reproduction and growth of some highly heterotrophic metabolic microorganisms such as β-Proteobacteria, γ-Proteobacteria and Acidobacteria which is not conducive to carbon sequestration. These results imply that the performance of pollutant interception and purification function of estuarine wetlands may weaken their carbon sequestration function to some extent. PMID:25993326

  11. Changes in the balance of soil respired CO2 (root vs. soil organic matter) during the Younger Dryas event; evidence from three European cave sites

    NASA Astrophysics Data System (ADS)

    Rudzka, Dominika; McDermott, Frank

    2010-05-01

    The main goal of this study was to understand better the response of soil carbon to a major climate transition and to examine the influence of variable temperature sensitivity of different carbon sources. New radiocarbon measurements for portions of three speleothems from European cave sites (La Garma and El Pindal caves, N. Spain; Sofular cave, Turkey) deposited during the late-Glacial to early Holocene were used to investigate these processes. These data were used to improve the interpretation of δ13C in stalagmites, which can be influenced not only by the temperature and moisture changes (reflected in major climatic transitions), but also by several different processes (e.g. variable degassing and limestone dissolution, soil evolution, hydrological effects). Pollen data indicate that C3 plants persisted at all three sites since the late-Glacial. There is however a marked increase in δ13C during the Younger Dryas (YD) in all three stalagmites, indicating a climate driven change in carbon cycling dynamics. In principle this change could reflect stronger degassing due to drier conditions or more closed system behaviour (higher 'dead carbon proportion' (dcp) due to greater limestone dissolution). Closed system modeling indicates that greater limestone dissolution should result in higher δ13C and lower initial 14C activity as a result of dilution by 'dead' carbon. In practice, initial 14C activity of stalagmite carbonate during the YD in all three speleothems follows the atmospheric 14C age plateau, indicating open system behaviour, with little evidence for lowering of 14C activity that would be expected to result from enhanced limestone dissolution. This is interpreted as a change in the balance of soil-respired CO2. Soil CO2 is a combination of soil organic matter (SOM) and plant-root respiration. In the latter process, plants cycle only ambient atmospheric CO2 (relatively high 14C activity). By contrast, microbial decomposition of soil organic matter typically

  12. Post-Fire Soil Respiration in Relation to the Burnt Wood Management

    NASA Astrophysics Data System (ADS)

    Marañón Jiménez, Sara; Castro, J.; Kowalski, A.; Serrano-Ortiz, P.; Ruiz, B.; Sancez-Canete, Ep; Zamora, R.

    2010-05-01

    Wildfires are the main cause of forests and understory destruction in Mediterranean areas. One of the most dramatic consequences is the perturbation of carbon fluxes. A high percentage of the CO2 emitted by the ecosystem after a wildfire is due to soil respiration, which represents the most important uncertainty in the global carbon cycle. In this study we have quantified the soil respiration and its seasonal variability in reforested pine forests in the National and Natural Park of Sierra Nevada which were burned in September of 2005. Measurement campaigns were carried out along two years in two experimental plots at different altitudinal levels (1500 and 2200 m a.s.l.), in which three post-fire silvicultural treatments of burned wood were established: 1) "Non-Intervention" (NI), leaving all of the burnt trees standing. 2) "Cut plus Lopping" (CL), a treatment where most of the trees were cut and felled, with the main branches also lopped off, but leaving all the cut biomass in situ covering partially the ground surface 3) "Salvage Logging" (SL), all trees were cut and the trunks and branches were removed. Soil respiration was highly determined by the effects derived of the altitudinal level, with the highest values at the lowest altitude. The seasonal precipitation regime had also a key role. Soil respiration kept a basal level during the summer drought, during this period the response to the altitudinal level and post-fire treatments were reduced. On the other hand, soil respiration boosted after rain events, when the differences between treatments became more pronounced. In general, especially under these conditions of absence of water limitation, the post-fire burnt wood treatment with the highest CO2 fluxes was that in which all the burnt wood biomass remained covering partially the soil surface ("Cut plus Lopping") while the lowest values were registered in the treatment in which the soil was bared ("Salvage Logging"). Results of this study are especially

  13. Do traits of invasive species influence decomposition and soil respiration of disturbed ecosystems?

    NASA Astrophysics Data System (ADS)

    Wells, A. J.; Balster, N. J.

    2009-12-01

    Large-scale landscape disturbances typically alter the terrestrial carbon cycle leading to shifts in pools of soil carbon. Restoration of disturbed landscapes with prairie vegetation has thus been practiced with the intent of increasing carbon accrual in soils. However, since disturbed soils are prone to invasion by non-native invasive species, many ecological restorations have resulted in unexpected outcomes, which may be explained by differences in plant traits such as tissue quality and biomass allocation. Typically, the tissue of invasive species has lower C:N ratios relative to native species, and consequently, faster decomposition rates, which potentially can alter the balance in soil carbon. The primary objective of this research was to compare the effects of native prairie species versus non-native invasive species on the carbon cycling within a novel environment: a recently dewatered basin in southwestern Wisconsin following dam removal. We hypothesized that a higher invasive to native species ratio would result in faster litter decomposition and a higher rate of soil respiration. To test this hypothesis, we seeded newly exposed sediments with native prairie seeds in 2005, annually collected aboveground plant biomass (by species per plot), calculated decomposition rate of native and invasive litter (underneath both canopy types), and measured soil respiration during the growing season of 2009. After four years of seeding, the aboveground biomass of the native vegetation has increased significantly (p < 0.01) from 14.4 to 351 g m-2 while invasive species biomass has decreased from 459 to 296 g m-2. Senesced tissue from mixed native species had a higher C:N ratio, 27:1 (43% C: 1.6% N), than tissue from mixed invasive species, 24:1 (35% C: 1.5% N). However, after 7 months, we found that the rate of decomposition depended on both litter type and plant canopy type (p < 0.01); invasive plant tissue had a slightly faster decomposition rate than the native litter

  14. Parameters of microbial respiration in soils of the impact zone of a mineral fertilizer factory

    NASA Astrophysics Data System (ADS)

    Zhukova, A. D.; Khomyakov, D. M.

    2015-08-01

    The carbon content in the microbial biomass and the microbial production of CO2 (the biological component of soil respiration) were determined in the upper layer (0-10 cm) of soils in the impact zone of the OJSC Voskresensk Mineral Fertilizers, one of the largest factories manufacturing mineral fertilizers in Russia. Statistical characteristics and schematic distribution of the biological parameters in the soil cover of the impact zone were analyzed. The degree of disturbance of microbial communities in the studied objects varied from weak to medium. The maximum value (0.44) was observed on the sampling plot 4 km away from the factory and 0.5 km away from the place of waste (phosphogypsum) storage. Significantly lower carbon content in the microbial biomass and its specific respiration were recorded in the agrosoddy-podzolic soil as compared with the alluvial soil sampled at the same distance from the plant. The effects of potential soil pollutants (fluorine, sulfur, cadmium, and stable strontium) on the characteristics of soil microbial communities were described with reliable regression equations.

  15. Short Term Soil Respiration Response to Fire in a Semi-arid Ecosystem

    NASA Astrophysics Data System (ADS)

    Rozin, A. G.

    2015-12-01

    In the Intermountain West (USA), fire is an important driver of carbon cycling in the environment. Increasing frequency and severity of fires, either through management actions or wildfires, is expected with changing climates in the Western United States. When burning is used as a management tool, it may be beneficial and control the growth of nuisance vegetation, promote the regeneration of grasses and forage species, and reduce hazardous fuel loads to minimize the risk of future wildfires. However, high intensity wildfires often have a negative effect, resulting in a loss of carbon storage and a shift of vegetation communities. This delays recovery of the ecosystem for years or decades and alters the historic fire regime. A 2000 acre prescribed burn in the Reynolds Creek Critical Zone Observatory provided the opportunity to quantify pre and post-burn soil carbon stores and soil carbon losses by heterotrophic respiration. Pre and post-burn soil samples were collected for physical and biogeochemical characterization to quantify substrate availability and possible limitations for heterotrophic respiration. CO2 fluxes were continuously monitored in situ before and immediately after the fire to understand the short-term response of soil respiration to varying burn severities.

  16. Soil respiration contributes substantially to urban carbon fluxes in the greater Boston area.

    PubMed

    Decina, Stephen M; Hutyra, Lucy R; Gately, Conor K; Getson, Jackie M; Reinmann, Andrew B; Short Gianotti, Anne G; Templer, Pamela H

    2016-05-01

    Urban areas are the dominant source of U.S. fossil fuel carbon dioxide (FFCO2) emissions. In the absence of binding international treaties or decisive U.S. federal policy for greenhouse gas regulation, cities have also become leaders in greenhouse gas reduction efforts through climate action plans. These plans focus on anthropogenic carbon flows only, however, ignoring a potentially substantial contribution to atmospheric carbon dioxide (CO2) concentrations from biological respiration. Our aim was to measure the contribution of CO2 efflux from soil respiration to atmospheric CO2 fluxes using an automated CO2 efflux system and to use these measurements to model urban soil CO2 efflux across an urban area. We find that growing season soil respiration is dramatically enhanced in urban areas and represents levels of CO2 efflux of up to 72% of FFCO2 within greater Boston's residential areas, and that soils in urban forests, lawns, and landscaped cover types emit 2.62 ± 0.15, 4.49 ± 0.14, and 6.73 ± 0.26 μmolCO2 m(-2) s(-1), respectively, during the growing season. These rates represent up to 2.2 times greater soil respiration than rates found in nearby rural ecosystems in central Massachusetts (MA), a potential consequence of imported carbon amendments, such as mulch, within a general regime of landowner management. As the scientific community moves rapidly towards monitoring, reporting, and verification of CO2 emissions using ground based approaches and remotely-sensed observations to measure CO2 concentrations, our results show that measurement and modeling of biogenic urban CO2 fluxes will be a critical component for verification of urban climate action plans. PMID:26914093

  17. A comparison of trenched plot techniques for partitioning soil respiration

    SciTech Connect

    Bond-Lamberty, Benjamin; Bronson, Dustin; Bladyka, Emma; Gower, Stith T.

    2011-07-16

    Partitioning the soil surface CO{sub 2} flux (R{sub S}) flux is an important step in understanding ecosystem-level carbon cycling, given that R{sub S} is poorly constrained and its source components may have different responses to climate change. Trenched plots are a classic method of separating the R{sub S} source fluxes, but labor-intensive and may cause considerable disturbance to the soil environment. This study tested if various methods of plant suppression in trenched plots affected R{sub S} fluxes, quantified the R{sub S} response to soil temperature and moisture changes, and estimated the heterotrophic contribution to R{sub S}. It was performed in a boreal black spruce (Picea mariana) plantation, using a complete randomized design, during the 2007 growing season (May-November). Trenched plots had significantly lower R{sub S} than control plots, with differences appearing {approx}100 days after trenching; spatial variability doubled after trenching but then declined throughout the experiment. Most trenching treatments had significantly lower (by {approx}0.5 {mu}mol m{sup -2} s{sup -1}) R{sub S} than the controls, and there was no significant difference in R{sub S} among the various trenching treatments. Soil temperature at 2 cm explained more R{sub S} variability than did 10-cm temperature or soil moisture. Temperature sensitivity (Q10) declined in the control plots from {approx}2.6 (at 5 C) to {approx}1.6 (at 15 C); trenched plots values were higher, from 3.1 at 5 C to 1.9 at 15 C. We estimated R{sub S} for the study period to be 241 {+-} 40 g C m{sup -2}, with roots contributing 64% of R{sub S} after accounting for fine root decay, and 293 g C m{sup -2} for the entire year. These findings suggest that laborious hand weeding of vegetation may be usefully replaced by other methods, easing future studies of this large and poorly-understood carbon flux.

  18. In-pot evaluation of different composted and pelletized organic fertilizers on soil carbon dioxide efflux and basal respiration

    NASA Astrophysics Data System (ADS)

    Opsi, Francesca; Cavallo, Eugenio; Cocco, Stefania; Corti, Giuseppe

    2013-04-01

    Climate change is one of the most important environmental problems and it is closely related to concentration changes of greenhouse gases (GHG) in the atmosphere, mainly due to anthropogenic activities. As a consequence, measures have been taken to reduce GHG emissions, some of which are associated with agriculture, as well as to the enhancement of soil carbon storage. Modern intensive farming activities have also raised problems related to the safe disposal of large volume of animal waste, such as pig slurry, where the excessive land spreading can lead to water pollution and GHG evolution to the atmosphere. Composting is a great environmentally sustainable option for recycling agricultural by-products, and pelletisation is a promising technology to reduce the large volume of mature composted material in pelleted fertilizers, more suitable for long-distance transport. This study consisted of a pot-incubation experience carried out in a greenhouse of the National Research Council of Italy, under controlled conditions. The aim of the research was to investigate the effect of a composted swine solid fraction (CS, 13% w/w) and swine solid fraction blended with sawdust and composted (CSS, 9% w/w), both also as a result of pelletisation process (CSP, 12% w/w and CSSP, 8% w/w, respectively), on soil organic matter mineralization and basal respiration. Results were obtained by monitoring CO2 efflux, basal respiration and microbial biomass C on amended soil, freshly collected in a vineyard planted on a Typic Ustorthent, fine-loamy, mixed, calcareous, mesic. Samples, adjusted and maintained to about 50-60% of water holding capacity, were conditioned at 25±3 °C for 31 days of incubation. The CO2 fluxes showed a high production at the initial stage of incubation, where differences among treatments were well-rendered. CSSP produced the highest values, while CSS showed values as lower as about 45%. Intermediate values, and similar to those found in the soil sample used as

  19. Separating Autotrophic and Heterotrophic Contributions to Soil Respiration in Maize-Based Agroecosystems Using Stable Carbon Isotope Ratio Mass Spectrometry.

    NASA Astrophysics Data System (ADS)

    Amos, B.; Walters, D. T.; Madhavan, S.; Arkebauer, T. J.; Scoby, D. L.

    2005-12-01

    Any effort to establish a carbon budget for a growing crop by means of a thorough accounting of all C sources and sinks will require the ability to discriminate between autotrophic and heterotrophic contributions to soil surface CO2 flux. Autotrophic soil respiration (Ra) is defined as combined root respiration and the respiration of soil microorganisms residing in the rhizosphere and using root-derived carbohydrates as an energy source, while heterotrophic respiration (Rh) is defined as the respiration of soil microorganisms and macroorganisms not directly under the influence of the live root system and using SOM as an energy source. We partition soil surface CO2 flux into its autotrophic and heterotrophic components by combining root exclusion with stable carbon isotope techniques in production scale (~65 ha) maize-based agroecosystems. After flux measurements, small chambers are placed on collars in both root excluded shields and in non-root excluded soil, ambient headspace CO2 is removed using a soda lime trap, and soil-respired C is allowed to collect in the chambers. Soil respiration samples are then collected in 12mL evacuated exetainers and analyzed for δ13C by means of a Finnigan Delta-S isotope ratio mass spectrometer interfaced with a Thermo Finnigan GasBench II and a cryogenic trap to increase CO2 concentration. These δ13C measurements were made throughout the 2005 growing season in maize fields representing three agroecosystems: irrigated continuous maize, irrigated maize-soybean rotation, and rainfed maize soybean rotation. Estimates of autotrophic and heterotrophic soil respiration along with other results of this study will be presented.

  20. Remote sensing-based estimation of annual soil respiration at two contrasting forest sites

    SciTech Connect

    Gu, Lianhong; Huang, Ni; Black, T. Andrew; Wang, Li; Niu, Zheng

    2015-11-23

    Soil respiration (Rs), an important component of the global carbon cycle, can be estimated using remotely sensed data, but the accuracy of this technique has not been thoroughly investigated. In this article, we proposed a methodology for the remote estimation of annual Rs at two contrasting FLUXNET forest sites (a deciduous broadleaf forest and an evergreen needleleaf forest).

  1. Reduced temperature sensitivity of soil respiration after a 17-year climate change experiment

    NASA Astrophysics Data System (ADS)

    Bond-Lamberty, B. P.; Bailey, V. L.; Fansler, S.; Liu, C.; Smith, J. L.; Bolton, H.

    2012-12-01

    In 1994, a reciprocal soil transplant experiment was initiated between two elevations (310 m, warmer and drier, and 844 m, cooler and wetter) on Rattlesnake Mountain in southeastern Washington, USA, testing whether the microbial and biochemical dynamics that developed under cool, moist conditions would be destabilized under hot, dry conditions. In March 2012 we resampled the original transplanted soils to study longer-term changes in microbial community composition, soil C and N dynamics, and soil physical structure. These resampled cores were randomly assigned to climate-control chambers simulating the lower or upper site climates. We measured respiration throughout a 100-day incubation, coupled with biogeochemical analyses, to examine how these soils had responded to environmental changes over 17 years. Temperature and soil moisture were the primary drivers of CO2 evolution, but transplant source and destination both exerted significant effects. Most strikingly, respiration from cores originally from the hotter, low-elevation site that spent 17 years at the upper site exhibited almost no temperature sensitivity (Q10=1.07, 13-33 °C). Cores from the upper site had more carbon (~1.1% versus 0.8%), but equivalent C:N ratios, while soils incubated in the 'upper' chamber had greater N-acetylglucosaminidase and β-glucosidase potentials. Tomographic reconstructions revealed that porosity, moisture content, grain size distribution, and organic C were highly heterogeneous, consistent with the observed macro-scale variability. These results suggest that the upper-site soils were more resilient to the 1994 transplant, but that there is a significantly altered microbial community in the transplanted soils, particularly the lower-to-upper cores, that has not recovered almost two decades after the original experiment. This raises more general questions of how current climate change will affect soil resistance to future perturbations, and how confidently we can model this

  2. Response of soil respiration to climate across biofuel crops and land use histories

    NASA Astrophysics Data System (ADS)

    Su, Y.; Chen, J.; Shao, C.; Shen, W.; Zenone, T.; John, R.; Deal, M.; Hamilton, S. K.; Robertson, G. P.

    2013-12-01

    Land use change (LUC) due to the worldwide increasing production of biofuel crops creates carbon debt that would require decades to repay. The payback time depends on the net ecosystem exchange (NEE) of CO2 and more determined by the carbon loss, such as soil respiration, than photosynthesis offset. Soil respiration is not only an important part of ecosystem respiration, but is also highly correlated with ecosystem production, via substrate subsidies from plants. Both autotrophic and heterotrophic soil respiration were regulated by climated-induced factors (e.g. soil temperature and soil water content) and also affected by substrate supply. In 2009, three sites in conservation reserve program (CRP) and conventional corn-soybean rotation agricultural lands (AG), were converted to soybean production, in experimental sites at Kellogg Biological Station, MI. In 2010, the three sites of differential previous land uses were then converted to corn (Cr), switchgrass (Sw) and prairie mixture (Pr) production. A reference site has been maintained CRP status since then. We used chamber-based method to assess total and heterotrophic soil respirations rate (SRRt and SRRh) from control treatment (C) and root exclusion treatment (E) at all sites, in 2011 and 2012, to explore how soil respiration rate (SRR) respond to the change of abiotic and biotic factors. Our results show that soil temperature (Ts) are important factors that affect SRR patterns. At the beginning of growing season, SRRs are low (average SRRt and SRRh are 3.19 and 3.11 umol CO2/m2s, respectively, on April 10th, 2011) when soil temperature is low. SRRs in general increased over time in a year, peaked in late July- early August, 1-2 weeks after soil temperature arrive its peak (maximum average SRRt and SRRh are 8.64 and 5.68, respectively, on August 3rd/4th, 2011). Soil water content (VWC) did not affect the time of SRR peak but limited its amount; when VWCs were extremely low in 2012 (average VWC at C and E

  3. Soil CO2 respiration: Comparison of chemical titration, CO2 IRGA analysis and the Solvita gel system

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The purpose of this research is to compare the results of measured soil CO2 respiration using three methods: (1) titration method; (2) Infrared gas analysis (IRGA); and (3) the Solvita gel system for soil CO2 analysis. We acquired 36 soil samples from across the USA for comparison which ranged in pH...

  4. Patterns and Drivers of Soil Respiration under Long-Term Citrus reticulate in Southern China.

    PubMed

    Zhang, Yan-Jie; Zhang, Su-Yan; Yang, Jie; Yan, Yue; Fu, Xiang-Ping; Lu, Shun-Bao

    2015-01-01

    Soil respiration (Rs) is a major source of carbon emission in terrestrial ecosystems. Despite the fact that the influence of land use practice on Rs has been widely studied, the patterns and drivers on Rs of Citrus reticulata cultivation, a worldwide land use practice are unclear. In this current study, we investigated the influence of long-term cultivation of Citrus reticulata (CO) and of CO intercropped with soybean (CB) on soil nutrients, water availability, and Rs in southern China. Results indicated that after 21 years of cultivation, CO and CB significantly increased total soil carbon (TC), total soil nitrogen (TN), and soil organic matter (OM) at 0-20 cm and 20-40 cm, both at upslope and downslope compared with bare soil (CK). However, soil moisture (SM), dissolved organic carbon (DOC), and microbial biomass carbon (MBC) decreased under CB. In addition, no significant variation was found in soil pH between CK, CO, and CB. Across incubation time (56 days), Rs decreased exponentially with incubation time and CB showed the highest Rs rate irrespective of soil depth or topography. Linear regression further showed TC and TN as the two major factors influencing Rs upslope, while DOC was the dominant factor in regulating Rs downslope. These findings demonstrated that long-term cultivation of citrus significantly changed soil nutrients, water availability, and Rs rate. PMID:26368561

  5. Patterns and Drivers of Soil Respiration under Long-Term Citrus reticulate in Southern China

    PubMed Central

    Zhang, Yan-Jie; Zhang, Su-Yan; Yang, Jie; Yan, Yue; Fu, Xiang-ping; Lu, Shun-Bao

    2015-01-01

    Soil respiration (Rs) is a major source of carbon emission in terrestrial ecosystems. Despite the fact that the influence of land use practice on Rs has been widely studied, the patterns and drivers on Rs of Citrus reticulata cultivation, a worldwide land use practice are unclear. In this current study, we investigated the influence of long-term cultivation of Citrus reticulata (CO) and of CO intercropped with soybean (CB) on soil nutrients, water availability, and Rs in southern China. Results indicated that after 21 years of cultivation, CO and CB significantly increased total soil carbon (TC), total soil nitrogen (TN), and soil organic matter (OM) at 0–20 cm and 20–40 cm, both at upslope and downslope compared with bare soil (CK). However, soil moisture (SM), dissolved organic carbon (DOC), and microbial biomass carbon (MBC) decreased under CB. In addition, no significant variation was found in soil pH between CK, CO, and CB. Across incubation time (56 days), Rs decreased exponentially with incubation time and CB showed the highest Rs rate irrespective of soil depth or topography. Linear regression further showed TC and TN as the two major factors influencing Rs upslope, while DOC was the dominant factor in regulating Rs downslope. These findings demonstrated that long-term cultivation of citrus significantly changed soil nutrients, water availability, and Rs rate. PMID:26368561

  6. An isotopic investigation of the temperature response of young and old soil organic matter respiration

    NASA Astrophysics Data System (ADS)

    Burns, Nancy; Cloy, Joanna; Garnett, Mark; Reay, David; Smith, Keith; Otten, Wilfred

    2010-05-01

    The effect of temperature on rates of soil respiration is critical to our understanding of the terrestrial carbon cycle and potential feedbacks to climate change. The relative temperature sensitivity of labile and recalcitrant soil organic matter (SOM) is still controversial; different studies have produced contrasting results, indicating limited understanding of the underlying relationships between stabilisation processes and temperature. Current global carbon cycle models still rely on the assumption that SOM pools with different decay rates have the same temperature response, yet small differences in temperature response between pools could lead to very different climate feedbacks. This study examined the temperature response of soil respiration and the age of soil carbon respired from radiocarbon dated fractions of SOM (free, intra-aggregate and mineral-bound) and whole soils (organic and mineral layers). Samples were collected from a peaty gley soil from Harwood Forest, Northumberland, UK. SOM fractions were isolated from organic layer (5 - 17 cm) material using high density flotation and ultrasonic disaggregation - designated as free (< 1.8 g cm-3), intra-aggregate (< 1.8 g cm-3 within aggregates > 1.8 g cm-3) and mineral-bound (> 1.8 g cm-3) SOM. Fractions were analysed for chemical composition (FTIR, CHN analysis, ICP-OES), 14C (AMS), δ13C and δ15N (MS) and thermal properties (DSC). SOM fractions and bulk soil from the organic layer and the mineral layer (20 - 30 cm) were incubated in sealed vessels at 30 ° C and 10 ° C for 3 or 9 months to allow accumulation of CO2 sufficient for sampling. Accumulated respired CO2 samples were collected on zeolite molecular sieve cartridges and used for AMS radiocarbon dating. In parallel, material from the same fractions and layers were incubated at 10 ° C, 15 ° C, 25 ° C and 30 ° C for 6 months and sampled weekly for CO2 flux measurements using GC chromatography. Initial data have shown radiocarbon ages ranging

  7. Quantifying Components of Soil Respiration and Their Response to Abiotic Factors in Two Typical Subtropical Forest Stands, Southwest China

    PubMed Central

    Yu, Lei; Wang, Yujie; Wang, Yunqi; Sun, Suqi; Liu, Liziyuan

    2015-01-01

    Separating the components of soil respiration and understanding the roles of abiotic factors at a temporal scale among different forest types are critical issues in forest ecosystem carbon cycling. This study quantified the proportions of autotrophic (RA) and heterotrophic (RH) in total soil (RT) respiration using trenching and litter removal. Field studies were conducted in two typical subtropical forest stands (broadleaf and needle leaf mixed forest; bamboo forest) at Jinyun Mountain, near the Three Georges Reservoir in southwest China, during the growing season (Apr.–Sep.) from 2010 to 2012. The effects of air temperature (AT), soil temperature (ST) and soil moisture (SM) at 6cm depth, solar radiation (SR), pH on components of soil respiration were analyzed. Results show that: 1) SR, AT, and ST exhibited a similar temporal trend. The observed abiotic factors showed slight interannual variability for the two forest stands. 2) The contributions of RH and RA to RT for broadleaf and needle leaf mixed forest were 73.25% and 26.75%, respectively, while those for bamboo forest were 89.02% and 10.98%, respectively; soil respiration peaked from June to July. In both stands, CO2 released from the decomposition of soil organic matter (SOM), the strongest contributor to RT, accounted for over 63% of RH. 3) AT and ST were significantly positively correlated with RT and its components (p<0.05), and were major factors affecting soil respiration. 4) Components of soil respiration were significantly different between two forest stands (p<0.05), indicating that vegetation types played a role in soil respiration and its components. PMID:25680112

  8. A rapid in situ respiration test for measuring aerobic biodegradation rates of hydrocarbons in soil.

    PubMed

    Hinchee, R E; Ong, S K

    1992-10-01

    An in situ test method to measure the aerobic biodegradation rates of hydrocarbons in contaminated soil is presented. The test method provides an initial assessment of bioventing as a remediation technology for hydrocarbon-contaminated soil. The in situ respiration test consists of ventilating the contaminated soil of the unsaturated zone with air and periodically monitoring the depletion of oxygen (O2) and production of carbon dioxide (CO2) over time after the air is turned off. The test is simple to implement and generally takes about four to five days to complete. The test was applied at eight hydrocarbon-contaminated sites of different geological and climatic conditions. These sites were contaminated with petroleum products or petroleum fuels, except for two sites where the contaminants were primarily polycyclic aromatic hydrocarbons. Oxygen utilization rates for the eight sites ranged from 0.02 to 0.99 percent O2/hour. Estimated biodegradation rates ranged from 0.4 to 19 mg/kg of soil/day. These rates were similar to the biodegradation rates obtained from field and pilot studies using mass balance methods. Estimated biodegradation rates based on O2 utilization were generally more reliable (especially for alkaline soils) than rates based on CO2 production. CO2 produced from microbial respiration was probably converted to carbonate under alkaline conditions. PMID:1418936

  9. Landscape Soil Respiration Fluxes are Related to Leaf Area Index, Stand Height and Density, and Soil Nitrogen in Rocky Mountain Subalpine Forests

    NASA Astrophysics Data System (ADS)

    Berryman, E.; Bradford, J. B.; Hawbaker, T. J.; Birdsey, R.; Ryan, M. G.

    2015-12-01

    There is a recent multi-agency push for accurate assessments of terrestrial carbon stocks and fluxes in the United States. Assessing the state of the carbon cycle in the US requires estimates of stocks and fluxes at large spatial scales. Such assessments are difficult, especially for soil respiration, which dominates ecosystem respiration and is notoriously highly variable over space and time. Here, we report three consecutive years of measurement of soil respiration fluxes in three 1 km2 subalpine forest landscapes: Fraser Experimental Forest (Colorado), Glacier Lakes Ecosystems Experimental Site ("GLEES", Wyoming), and Niwot Ridge (Colorado). Plots were established following the protocol of the US Forest Service's Forest Inventory and Analysis (FIA) Program. Clusters of plots were distributed across the landscape in a 0.25 km grid pattern. From 2004 through 2006, measurements of soil respiration were made once monthly during the growing season and twice during snowpack coverage for each year. Annual cumulative soil respiration was 6.10 (+/- 0.21) Mg ha-1y-1 for Fraser, 6.55 (+/- 0.27) Mg ha-1y-1 for GLEES, and 6.97 (+/- 0.20) Mg ha-1y-1 for Niwot. Variability in annual cumulative soil respiration varied by less than 20% among the three subalpine forests, despite differences in terrain, climate, disturbance history and anthropogenic nitrogen deposition. We quantified the relationship between respiration fluxes and commonly-measured forest properties and found that soil respiration was nonlinearly related to leaf area index, peaking around 2.5 m2m-2 then slowly declining. Annual litterfall (FA) was subtracted from soil respiration (FR) to calculate total belowground carbon flux (TBCF), which declined with increasing tree height, density and soil nitrogen. This landscape analysis of soil respiration confirmed experimentally-derived principles governing carbon fluxes in forests: as trees age and get taller, and in high-fertility areas, carbon flux to roots declines

  10. Multi-Year Lags between Forest Browning and Soil Respiration at High Northern Latitudes

    SciTech Connect

    Bond-Lamberty, Benjamin; Bunn, Andrew G.; Thomson, Allison M.

    2012-11-26

    High-latitude northern ecosystems are experiencing rapid climate changes, and represent a large potential climate feedback because of their high soil carbon densities and shifting disturbance regimes. A significant carbon flow from these ecosystems is soil respiration (RS, the flow of carbon dioxide, generated by plant roots and soil fauna, from the soil surface to atmosphere), and any change in the high-latitude carbon cycle might thus be reflected in RS observed in the field. This study used two variants of a machine-learning algorithm and least squares regression to examine how remotely-sensed canopy greenness (NDVI), climate, and other variables are coupled to annual RS based on 105 observations from 64 circumpolar sites in a global database. The addition of NDVI roughly doubled model performance, with the best-performing models explaining ~62% of observed RS variability

  11. [Effects of simulated acid rain on respiration rate of cropland system with different soil pH].

    PubMed

    Zhu, Xue-zhu; Zhang, Gao-chuan; Li, Hui

    2009-10-15

    To evaluate the effects of acid rain on the respiration rate of cropland system, an outdoor pot experiment was conducted with paddy soils of pH 5.48 (S1), pH 6.70 (S1) and pH 8.18 (S3) during the 2005-2007 wheat-growing seasons. The cropland system was exposed to acid rain by spraying the wheat foliage and irrigating the soil with simulated rainwater of T1 (pH 6.0), T2 (pH 6.0, ionic concentration was twice as rainwater T1), and T3 (pH 4.4, ionic concentration was twice as rainwater T1), respectively. The static opaque chamber-gas chromatograph method was used to measure CO2 fluxes from cropland system. The results showed that acid rain affected the respiration rate of cropland system through crop plant, and the cropland system could adapt to acid rain. Acid rainwater significantly increased the average respiration rate in alkaline soil (S3) cropland system, while it had no significant effects on the average respiration rate in neutral soil (S2) and acidic soil (S1) cropland systems. During 2005-2006, after the alkaline soil cropland system was treated with rainwater T3, the average respiration rate was 23.6% and 27.6% higher than that of alkaline soil cropland system treated with rainwater T1 and T2, respectively. During March to April, the respiration rate was enhanced with the increase of rainwater ionic concentration, while it was dropped with the decrease of rainwater pH value in acidic soil cropland system. It was demonstrated that soil pH and crop plant played important roles on the respiration rate of cropland system. PMID:19968099

  12. Soil respiration following partial stand disturbance by tree girdling reveals a rapid rebound within a three-year period in a temperate forest

    NASA Astrophysics Data System (ADS)

    Levy, J.; Schuster, W.; Griffin, K. L.

    2012-12-01

    Disturbance events can greatly impact carbon cycling and subsequently alter the carbon storage service of a forest. The resulting atmosphere-biosphere feedback through CO2 makes understanding the carbon cycle response imperative for climate change research. The aim of this study was to examine the temporal response of soil respiration after a partial stand disturbance and to reassess the autotrophic contribution to soil respiration after three years at the Black Rock Forest (southeastern NY, USA). Tree girdling was used to initiate disturbance and create the following treatments: control (C), girdling all non-oaks (NO), girdling half of the oak trees (O50), girdling all the oaks (OG), and girdling all trees (ALL). Soil respiration was measured for three consecutive years. Respiratory rates on O50, OG, and ALL plots declined for two years following girdling before attaining a full rebound of belowground activity in the third year. Soil respiration on NO was similar to C for the duration of the study. The short lived respiratory response on O50, OG, and ALL aligns with patterns of reported NEP recovery after a pest or pathogen attack in other forested systems and suggests that belowground activity is resilient to disturbance. Respiratory responses among the various treatments were not proportional to the degree of disturbance experienced and varied through time. Based on the results from the first year of girdling, we previously estimated the autotrophic component to be 50 % of the total respiratory flux but continued declines in soil respiration rates into the second year provided an opportunity to make a more accurate estimate of 58 %.

  13. Water Consumption, Soil Temperature and Soil Respiration in Model Ecosystems of Young Oak Stands Treated by Air-warming and Drought

    NASA Astrophysics Data System (ADS)

    Kuster, Thomas; Arend, Matthias; Günthardt-Goerg, Madeleine S.; Schulin, Rainer

    2010-05-01

    , air-warming led to higher air temperatures at day time. But also the drought treatment increased air temperature due to reduced air chilling by evapotranspiration. Thus the highest temperatures were observed in the combined air-warming and drought treatment. Soil respiration was much lower under drought conditions than in control plots. No differences in soil respiration remained 10 days after rewetting of the soils, indicating that regeneration of biological soil activity was complete within short time. Air-warming had only little effect on soil respiration. We conclude that increased temperatures and extended drought periods, two important trends associated with global climate change, will have strong effects on forest ecosystems. Reduced soil water availability during drought periods will be a particular severe challenge for trees in temperate forests. In comparison to many other forest tree species in temperate humid regions, oaks have the advantage to sustain longer periods without rain and therefore may be well prepared to deal with future climate conditions. In a next step, we will use our results to model and simulate the potential future water regime of oak forests under various site conditions in different IPCC scenarios.

  14. Modeling soil respiration and variations of source components using a multi-factor global climate change experiment

    SciTech Connect

    Chen, Xiongwen; Post, Wilfred M; Norby, Richard J; Classen, Aimee T

    2011-01-01

    Soil respiration is an important component of the global carbon cycle and is highly responsive to changes in soil temperature and moisture. Accurate prediction of soil respiration and its changes under future climatic conditions requires a clear understanding of the processes involved. In spite of this, most current empirical soil respiration models incorporate just few of the underlying mechanisms that may influence its response. In this study, a new partial process-based component model built on source components of soil respiration was tested using data collected from a multi-factor climate change experiment that manipulates CO2 concentrations, temperature and precipitation. These results were then compared to results generated using several other established models. The component model we tested performed well across different treatments of global climate change. In contrast, some other models, which worked well predicting ambient environmental conditions, were unable to predict the changes under different climate change treatments. Based on the component model, the relative proportions of heterotrophic respiration (Rh) in the total soil respiration at different treatments varied from 0.33 to 0.85. There is a significant increase in the proportion of Rh under the elevated atmospheric CO2 concentration in comparison ambient conditions. The dry treatment resulted in higher proportion of Rh at elevated CO2 and ambient T than under elevated CO2 and elevated T. Also, the ratios between root growth and root maintenance respiration varied across different treatments. Neither increased temperature nor elevated atmospheric CO2 changed Q10 values significantly, while the average Q10 value at wet sites was significantly higher than it at dry sites. There was a higher possibility of increased soil respiration under drying relative to wetting conditions across all treatments based on monthly data, indicating that soil respiration may also be related to soil moisture at

  15. Soil microbial respiration and PICT responses to an industrial and historic lead pollution: a field study.

    PubMed

    Bérard, Annette; Capowiez, Line; Mombo, Stéphane; Schreck, Eva; Dumat, Camille; Deola, Frédéric; Capowiez, Yvan

    2016-03-01

    We performed a field investigation to study the long-term impacts of Pb soil contamination on soil microbial communities and their catabolic structure in the context of an industrial site consisting of a plot of land surrounding a secondary lead smelter. Microbial biomass, catabolic profiles, and ecotoxicological responses (PICT) were monitored on soils sampled at selected locations along 110-m transects established on the site. We confirmed the high toxicity of Pb on respirations and microbial and fungal biomasses by measuring positive correlations with distance from the wall factory and negative correlation with total Pb concentrations. Pb contamination also induced changes in microbial and fungal catabolic structure (from carbohydrates to amino acids through carboxylic malic acid). Moreover, PICT measurement allowed to establish causal linkages between lead and its effect on biological communities taking into account the contamination history of the ecosystem at community level. The positive correlation between qCO2 (based on respiration and substrate use) and PICT suggested that the Pb stress-induced acquisition of tolerance came at a greater energy cost for microbial communities in order to cope with the toxicity of the metal. In this industrial context of long-term polymetallic contamination dominated by Pb in a field experiment, we confirmed impacts of this metal on soil functioning through microbial communities, as previously observed for earthworm communities. PMID:26233741

  16. The immediate and prolonged effects of climate extremes on soil respiration in a mesic grassland

    NASA Astrophysics Data System (ADS)

    Hoover, David L.; Knapp, Alan K.; Smith, Melinda D.

    2016-04-01

    The predicted increase in the frequency and intensity of climate extremes is expected to impact terrestrial carbon fluxes to the atmosphere, potentially changing ecosystems from carbon sinks to sources, with positive feedbacks to climate change. As the second largest terrestrial carbon flux, soil CO2 efflux or soil respiration (Rs) is strongly influenced by soil temperature and moisture. Thus, climate extremes such as heat waves and extreme drought should have substantial impacts on Rs. We investigated the effects of such climate extremes on growing season Rs in a mesic grassland by experimentally imposing 2 years of extreme drought combined with midsummer heat waves. After this 2 year period, we continued to measure Rs during a recovery year. Two consecutive drought years reduced Rs by about 25% each growing season; however, when normal rainfall returned during the recovery year, formerly droughted plots had higher rates of Rs than control plots (up to +17%). The heat wave treatments had no effect on Rs, alone or when combined with drought, and during the growing season, soil moisture was the primary driver of Rs with little evidence for Rs temperature sensitivity. When compared to aboveground net primary production, growing season Rs was much less sensitive to drought but was more responsive postdrought. These results are consistent with the hypothesis that ecosystems become sources of CO2 during drought because carbon inputs (production) are decreased relatively more than outputs (respiration). Moreover, stimulation of Rs postdrought may lengthen the time required for net carbon exchange to return to predrought levels.

  17. SRF Vs. Rapeseed: Insights from soil respiration and combustion heat per area

    NASA Astrophysics Data System (ADS)

    Zurba, Kamal; Matschullat, Jörg

    2015-04-01

    Bioenergy crops may be important to mitigate global warming risks. They are a renewable energy source and have the potential to offset CO2 emissions by storing C in soils. In this study, a comparison between willow and poplar short rotation forestry (SRF) with rapeseed cultivation was made to estimate the ratio between the emitted quantities of carbon dioxide from soil (soil respiration) and the combustion heat obtained from the extracted products per hectare. This ratio is valuable because it delivers a three dimensional information: soil respiration (kg CO2), combustion heat values (GJ) and area of used land (ha). A manual static closed chamber (SEMACH-FG) was applied to measure CO2 emissions at the SRF and rapeseed sites during the growing season 2014 (April-October). Our results showed that poplar and willow SRF has a very low ratio comparing to rapeseed (157.78±12.03, 199.91±31.3 and 1128.14 kg CO2 GJ-1, respectively). We thus recommend poplar and willow SRF as renewable sources for bioenergy over the currently prevalent rapeseed production.

  18. Delayed recovery of soil respiration after wetting of dry soil further reduces C losses from a Norway spruce forest soil

    NASA Astrophysics Data System (ADS)

    Muhr, Jan; Borken, Werner

    2009-12-01

    This experiment investigated the effects of prolonged summer drought on soil respiration (SR) in a mountainous Norway spruce forest in south Germany. On three manipulation plots we excluded summer throughfall in the years of 2006/2007 and measured SR fluxes in comparison to three control plots. Using radiocarbon measurements we quantified the contribution of rhizosphere (RR) and heterotrophic respiration (HR) to total SR. In both manipulation years, mean CO2 emissions (±SE) from the throughfall exclusion (TE) plots were smaller than from the control plots with 5.7 t C ha-1 (±0.3) compared to 6.7 t C ha-1 (±0.2) in 2006 and 5.9 t C ha-1 (±0.3) compared to 7.0 t C ha-1 (±0.4) in 2007. Under control conditions, CO2 originated mainly from HR (60-95% of SR). Prolonged drought reduced HR, whereas RR was not affected or even increased slightly. Reduction of CO2 emissions on the TE plots was found up to 6 weeks after differences in matric potential conditions disappeared, possibly either because water repellency inhibited homogeneous rewetting of the organic horizons or because of severe damage to the microbial population. No evidence was found for the release of new, formerly protected substrates by preceding drought. Continuous measurements in 2008 (no manipulation) did not reveal increased CO2 emissions on the TE plots that could compensate for the reduction during the years 2006/2007. Based on our results, we postulate a negative feedback between increased frequency and magnitude of summer droughts and SR in Norway spruce stands.

  19. What determines the spatial variability of soil respiration and its temperature dependence (Q10) at catchment scale (Rur Catchment, Germany)?

    NASA Astrophysics Data System (ADS)

    Meyer, Nele; Welp, Gerhard; Amelung, Wulf

    2016-04-01

    Climate change is suspected to alter temperature, soil moisture, and nutrient inputs to the soil. These factors are supposed to strongly influence soil respiration. The degree by which respiration will respond to these changes is crucial for assessing future CO2 feedbacks to the atmosphere. We assume that the temperature sensitivity of soil respiration (Q10) differs spatially depending on land use, soil unit, and texture owing to their diverse properties of soil organic matter quantity and quality. We further hypothesize that the Q10 value is additionally regulated by soil moisture and nutrient status. On the basis of soil and land use maps we divided the Rur catchment (Western Germany, 2350 km²) into so called environmental soil classes (ESC) that combine each a unique combination of the factors land use, soil unit, and texture. We took nine samples from each of the 12 most common ESC's and incubated them at five temperatures (5-25°C), at four soil moisture levels (30-75% water holding capacity), and with an unfertilized and a fertilized treatment. So far, our results indicate that both soil respiration and the Q10 value are spatially highly variable with Q10 values ranging from 1 to 4. The Q10 value is altered by the level of soil moisture and decreases when soils are as moist as 75% water holding capacity. Fertilization has no effect on the Q10 value. Currently, we are processing the whole data-set to derive the effect of ESC's on the Q10 value. Recent data suggest that forest soils are more sensitive to warming than cropland soils.

  20. Integrating est.of ecosystem respiration from eddy covariance towers with automated measures of soil respiration: Exam. the dvlpt. and influence of hysteresis in soil respiratory fluxes along a woody plant gradient 2026

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The physiognomic shift in ecosystem structure from a grassland to a woodland may alter the sensitivity of CO2 exchange to variations in growing-season temperatures and precipitation inputs. One large component of ecosystem flux is the efflux of CO2 from the soil (soil respiration, Rsoil), which is ...

  1. The influence of soils on heterotrophic respiration exerts a strong control on net ecosystem productivity in seasonally dry Amazonian forests

    NASA Astrophysics Data System (ADS)

    Melton, J. R.; Shrestha, R. K.; Arora, V. K.

    2015-02-01

    Net ecosystem productivity of carbon (NEP) in seasonally dry forests of the Amazon varies greatly between sites with similar precipitation patterns. Correctly modeling the NEP seasonality with terrestrial ecosystem models has proven difficult. Previous modelling studies have mostly advocated for incorporating processes that act to reduce water stress on gross primary productivity (GPP) during the dry season, such as deep soils and roots, plant-mediated hydraulic redistribution of soil moisture, and increased dry season leaf litter generation which reduces leaf age and thus increases photosynthetic capacity. Recent observations, however, indicate that seasonality in heterotrophic respiration also contributes to the observed seasonal cycle of NEP. Here, we use the dynamic vegetation model CLASS-CTEM (Canadian Land Surface Scheme-Canadian Terrestrial Ecosystem Model) - without deep soils or roots, hydraulic redistribution of soil moisture, or increased dry season litter generation - at two Large-Scale Biosphere-Atmosphere Experiment (LBA) sites (Tapajós km 83 and Jarú Reserve). These LBA sites exhibit opposite seasonal NEP cycles despite reasonably similar meteorological conditions. Our simulations are able to reproduce the observed NEP seasonality at both sites. Simulated GPP, heterotrophic respiration, latent and sensible heat fluxes, litter fall rate, soil moisture and temperature, and basic vegetation state are also compared with available observation-based estimates which provide confidence that overall the model behaves realistically at the two sites. Our results indicate that representing the effect of soil moisture on heterotrophic respiration in terms of soil matric potential and constraining heterotrophic respiration when absolute soil matric potential is both low (wetter soils) and high (drier soils), with optimum conditions in between, allows %appropriately representing the influence of soil texture and depth, %through soil moisture, on seasonal patterns

  2. Respiration of 13C-Labeled Substrates Added to Soil in the Field and Subsequent 16S rRNA Gene Analysis of 13C-Labeled Soil DNA

    PubMed Central

    Padmanabhan, P.; Padmanabhan, S.; DeRito, C.; Gray, A.; Gannon, D.; Snape, J. R.; Tsai, C. S.; Park, W.; Jeon, C.; Madsen, E. L.

    2003-01-01

    Our goal was to develop a field soil biodegradation assay using 13C-labeled compounds and identify the active microorganisms by analyzing 16S rRNA genes in soil-derived 13C-labeled DNA. Our biodegradation approach sought to minimize microbiological artifacts caused by physical and/or nutritional disturbance of soil associated with sampling and laboratory incubation. The new field-based assay involved the release of 13C-labeled compounds (glucose, phenol, caffeine, and naphthalene) to soil plots, installation of open-bottom glass chambers that covered the soil, and analysis of samples of headspace gases for 13CO2 respiration by gas chromatography/mass spectrometry (GC/MS). We verified that the GC/MS procedure was capable of assessing respiration of the four substrates added (50 ppm) to 5 g of soil in sealed laboratory incubations. Next, we determined background levels of 13CO2 emitted from naturally occurring soil organic matter to chambers inserted into our field soil test plots. We found that the conservative tracer, SF6, that was injected into the headspace rapidly diffused out of the soil chamber and thus would be of little value for computing the efficiency of retaining respired 13CO2. Field respiration assays using all four compounds were completed. Background respiration from soil organic matter interfered with the documentation of in situ respiration of the slowly metabolized (caffeine) and sparingly soluble (naphthalene) compounds. Nonetheless, transient peaks of 13CO2 released in excess of background were found in glucose- and phenol-treated soil within 8 h. Cesium-chloride separation of 13C-labeled soil DNA was followed by PCR amplification and sequencing of 16S rRNA genes from microbial populations involved with 13C-substrate metabolism. A total of 29 full sequences revealed that active populations included relatives of Arthrobacter, Pseudomonas, Acinetobacter, Massilia, Flavobacterium, and Pedobacter spp. for glucose; Pseudomonas, Pantoea, Acinetobacter

  3. Biomarkers as Indicators of Respiration During Laboratory Incubations of Alaskan Arctic Tundra Permafrost Soils

    NASA Astrophysics Data System (ADS)

    Hutchings, J.; Schuur, E.; Bianchi, T. S.; Bracho, R. G.

    2015-12-01

    High latitude permafrost soils are estimated to store 1,330 - 1,580 Pg C, which account for ca. 40% of global soil C and nearly twice that of atmospheric C. Disproportionate heating of high latitude regions during climate warming potentially results in permafrost thaw and degradation of surficial and previously-frozen soil C. Understanding how newly-thawed soils respond to microbial degradation is essential to predicting C emissions from this region. Laboratory incubations have been a key tool in understanding potential respiration rates from high latitude soils. A recent study found that among the common soil measurements, C:N was the best predictor of C losses. Here, we analyzed Alaskan Arctic tundra soils from before and after a nearly 3-year laboratory incubation. Bulk geochemical values as well as the following biomarkers were measured: lignin, amino acids, n-alkanes, and glycerol dialkyl glycerol tetraethers (GDGT). We found that initial C:N did not predict C losses and no significant change in C:N between initial and final samples. The lignin acid to aldehyde (Ad:Al) degradation index showed the same results with a lack of C loss prediction and no significant change during the experiment. However, we did find that C:N and Ad:Al had a significant negative correlation suggesting behavior consistent with expectations. The failure to predict C losses was likely influenced by a number of factors, including the possibility that biomarkers were tracking a smaller fraction of slower cycling components of soil C. To better interpret these results, we also used a hydroxyproline-based amino acid degradation index and n-alkanes to estimate the contribution Sphagnum mosses to soil samples - known to have slower turnover times than vascular plants. Finally, we applied a GDGT soil temperature proxy to estimate the growing season soil temperatures before each incubation, as well as investigating the effects of incubation temperature on the index's temperature estimate.

  4. Exploratory Research - Using Volatile Organic Compounds to Separate Heterotrophic and Autotrophic Forest Soil Respiration

    SciTech Connect

    Roberts, Scott D; Hatten, Jeffrey A

    2015-02-09

    The initial focus of this project was to develop a method to partition soil respiration into its components (autotrophic, heterotrophic etc.) using the fingerprint of volatile organic compounds (VOCs) from soils. We were able to identify 63 different VOCs in our study; however, due to technical difficulties we were unable to take reliable measurements in order to test our hypotheses and develop this method. In the end, we changed the objectives of the project. Our new objectives were to characterize the effects of species and soil moisture regime on the composition of soil organic matter. We utilized the soils from the greenhouse experiment we had established for the soil VOC study and determined the lignin biomarker profiles of each of the treatments. We found that moisture had a significant effect on the carbon content of the soils with the low moisture treatments having higher carbon content than the high moisture treatments. We found that the relative yield of syringyl phenols (SP), ligin (Lig), and substituted fatty acids (SFA) were elevated in deciduous planted pots and reduced in conifer planted pots relative to plant-free treatments. Our results suggest nuttall oak preserved lignin and SFA, while loblolly pine lost lignin and SFA similarly to the plant free treatments. Since we did not find that the carbon concentrations of the soils were different between the species, nuttall oak probably replaced more native soil carbon than loblolly pine. This suggests that relative to loblolly pine, nuttall oak is a priming species. Since priming may impact soil carbon pools more than temperature or moisture, determining which species are priming species may facilitate an understanding of the interaction that land use and climate change may have on soil carbon pools.

  5. Effects of seagulls on ecosystem respiration, soil nitrogen and vegetation cover on a pristine volcanic island, Surtsey, Iceland

    NASA Astrophysics Data System (ADS)

    Sigurdsson, B. D.; Magnusson, B.

    2010-03-01

    When Surtsey rose from the North Atlantic Ocean south of Iceland in 1963, it became a unique natural laboratory on how organisms colonize volcanic islands and form ecosystems with contrasting structures and functions. In July, 2004, ecosystem respiration rate (Re), soil properties and surface cover of vascular plants were measured in 21 permanent research plots distributed among the juvenile communities of the island. The plots were divided into two main groups, inside and outside a seagull (Larus spp.) colony established on the island. Vegetation cover of the plots was strongly related to the density of gull nests. Occurrence of nests and increased vegetation cover also coincided with significant increases in Re, soil carbon, nitrogen and C:N ratio, and with significant reductions in soil pH and soil temperatures. Temperature sensitivity (Q10 value) of Re was determined as 5.3. When compared at constant temperature the Re was found to be 59 times higher within the seagull colony, similar to the highest fluxes measured in drained wetlands or agricultural fields in Iceland. The amount of soil nitrogen, mainly brought onto the island by the seagulls, was the critical factor that most influenced ecosystem fluxes and vegetation development on Surtsey. The present study shows how ecosystem activity can be enhanced by colonization of animals that transfer resources from a nearby ecosystem.

  6. The pulsed response of soil respiration to precipitation in an African savanna ecosystem: a coupled measurement and modeling approach

    NASA Astrophysics Data System (ADS)

    Fan, Z.; Neff, J. C.; Hanan, N. P.

    2014-12-01

    Savannas cover 60% of the African continent and play an essential role in the global carbon (C) cycle. To better characterize the physical controls over soil respiration in these settings, half-hourly observations of volumetric soil-water content, temperature, and the concentration of carbon dioxide (CO2) at different soil depths were continually measured from 2005 to 2007 under trees ("sub-canopy") and between trees ("inter-canopy") in a savanna vegetation near Skukuza, Kruger National Park, South Africa. The measured soil climate and CO2 concentration data were assimilated into a process-based model that estimates the CO2 production and flux with coupled dynamics of dissolved organic C (DOC) and microbial biomass C. Our results show that temporal and spatial variations in CO2 flux were strongly influenced by precipitation and vegetation cover, with two times greater CO2 flux in the sub-canopy plots (~2421 g CO2 m-2 yr-1) than in the inter-canopy plots (~1290 g CO2 m-2 yr-1). Precipitation influenced soil respiration by changing soil temperature and moisture; however, our modeling analysis suggests that the pulsed response of soil respiration to precipitation [known as "Birch effect (BE)"] is a key control on soil fluxes at this site. At this site, BE contributed to approximately 50% and 65% of heterotrophic respiration or 20% and 39% of soil respiration in the sub-canopy and inter-canopy plots, respectively. These results suggest that pulsed response of respiration to precipitation is an important component of the C cycle of savannas and should be considered in both measurement and modeling studies of carbon exchange in similar ecosystems.

  7. Soil respiration under mature deciduous forest trees after 7 years of CO2 enrichment

    NASA Astrophysics Data System (ADS)

    Bader, Martin; Körner, Christian

    2010-05-01

    The anthropogenic rise in atmospheric CO2 is expected to impact carbon fluxes not only at ecosystem level but also at the global scale by altering carbon cycle processes in soils. At the Swiss Canopy Crane (SCC), we examined how 7 years of free air CO2 enrichment (FACE) affected soil CO2 dynamics in a c. 100-year-old mixed deciduous forest. The use of 13C-depleted CO2 for canopy enrichment allowed us to trace the flow of recently fixed carbon (C). In the seventh year of growth at ~550 ppm CO2, soil respiratory CO2 consisted of 39% labelled C. During the growing season, soil air CO2 concentration was significantly enhanced under CO2-exposed trees. However, elevated CO2 failed to stimulate cumulative soil respiration (Rs) over the growing season. We found periodic reductions as well as increases in instantaneous rates of Rs in response to elevated CO2, depending on soil temperature and soil volumetric water content (VWC; significant 3-way interaction). During wet periods, soil water savings under CO2-enriched trees led to excessive VWC (>45%) that suppressed Rs. Elevated CO2 stimulated Rs only when VWC was ≤40% and concurrent soil temperature was high (>15 °C). Seasonal Q10 estimates of Rs were significantly lower under elevated (Q10 = 3.30) compared to ambient CO2 (Q10 = 3.97). However, this effect disappeared when 3 consecutive sampling dates of extremely high VWC were disregarded. This suggests that elevated CO2 affected Q10 mainly indirectly through changes in VWC. Fine root respiration did not differ significantly between treatments but soil microbial biomass (Cmic) increased by 14% under elevated CO2 (marginally significant). Our findings do not indicate enhanced soil C emissions in such stands under future atmospheric CO2. It remains to be shown whether C losses via leaching of dissolved organic or inorganic C (DOC, DIC) help to balance the carbon budget in this forest.

  8. Soil Organic Carbon Sources of Respired CO2 in a Mid-successional North Temperate Forest

    NASA Astrophysics Data System (ADS)

    Medina, N. L.; Hatton, P. J.; Le Moine, J.; Nadelhoffer, K. J.

    2015-12-01

    Given that soil organic matter (SOM) is the largest global terrestrial carbon (C) pool, some fractions of which have turnover times of centuries to millennia, it is critical to understand the mechanisms by which higher net primary productivity (NPP) and higher litter inputs, in the future, as predicted by some models, might alter the potentials of forest soils to serve as long-term C sinks. Here, we use a 10-year-old site in the DIRT (Detritus Input and Removal Treatments) network of litter manipulations to compare plots in a forested, northern-temperate sandy soil that were subjected to double-leaf-litter additions (DL) and both root- and leaf-litter removals (no inputs, NI) to non-manipulated controls. Previous data show that rather than increasing soil organic carbon (SOC) stocks, plots receiving doubled litter inputs lose SOC at rates similar to losses in Control soils. To trace the source of extra mineralized SOC, we analyzed field CO2 effluxes for δ13C and characterized SOC of varying degrees of organo-mineral association with sequential density fractionations. Soils in DL plots respired significantly faster (p=0.095) and proportionally more (p=0.015) than control soils over the course of July, August, and October 2014. This suggests a greater fresh litter contribution to soil efflux in DL than in Control plots after 10 years of treatment. Preliminary data show that intermediate (1.85 - 2.4 g/mL) and dense (>2.4 g/mL) fractions are relatively larger in DL than in Control soils. This suggests that the addition C from doubled litter could be more rapidly transferred into those more dense fractions, or that higher litter inputs prime the decomposition of lighter particulate SOC forms, leading to a relative increase of the dense organo-mineral associations. Using δ13C values to parameterize a multi-source mixing model, we partition the fate of both fresh litter and partially-decomposed SOC and will present on the modeled relative contributions of various

  9. The impact of corn stover removal on N2O emission and soil respiration: An investigation with automated chambers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Corn stover removal, whether for silage, bedding, or bioenergy production, could have a variety of environmental consequences through its effect on soil processes, particularly N2O production and soil respiration. Because these effects may be episodic in nature, weekly snapshots with static chambers...

  10. SEASONAL CHANGES IN ROOT AND SOIL RESPIRATION OF OZONE-EXPOSED PONDEROSA PINE (PINUS PONDEROSA) GROWN IN DIFFERENT SUBSTRATES

    EPA Science Inventory

    Exposure to(ozone 0-3)has been shown to decrease the allocation of carbon to tree roots. Decreased allocation of carbon to roots might disrupt root metabolism and rhizosphere organisms. The effects of soil type and shoot 0, exposure on below-ground respiration and soil microbial ...

  11. Impact of Organic Amendments with and Without Mineral Fertilizers on Soil Microbial Respiration

    NASA Astrophysics Data System (ADS)

    Gilani, S. S.; Bahmanyar, M. A.

    A field experiment was conducted to study the effects of Sewage Sludge (SS), Municipal Waste Compost (MWC) and Vermicompost (VC) with and without chemical fertilizer (Urea, 50 kg ha-1 + Potassium sulfate, 100 kg ha-1 + Triple super phosphate, 127.5 kg ha-1) on Soil Microbial Respiration (SMR) and Total Organic Carbon (TOC) in a soil cropped to soybean. Experiment was arranged in a complete block design with three replications. Organic amendments were added to soil at rate of 0 (control treatment), 20 and 40 Mg ha-1. Furthermore each level of organic fertilizers with ½ normal of chemical fertilizer was also enriched. Soil samples were taken after one year of fertilization. Results illustrated that application of organic amendments increased TOC and SMR and soybean yield compared to control and chemical fertilizer treatments. Sewage sludge amended soils showed higher SMR, TOC and soybean yield than that of other organic amendment treatments. An increasing trend was observed in all studied parameters, as rates of application increased. All parameters were greater in treatments receiving a combination of chemical fertilizers and organic amendments (enriched treatments) compared to soils receiving organic amendments alone. Results obtained by discriminate analysis indicated that rates of application were more effective to create discriminating among treatments. This study showed that TOC was significantly correlated with SMR. Significant correlation was also observed between SMR and soybean yield.

  12. Temperature sensitivity of soil microbial communities: An application of macromolecular rate theory to microbial respiration

    NASA Astrophysics Data System (ADS)

    Alster, Charlotte J.; Koyama, Akihiro; Johnson, Nels G.; Wallenstein, Matthew D.; Fischer, Joseph C.

    2016-06-01

    There is compelling evidence that microbial communities vary widely in their temperature sensitivity and may adapt to warming through time. To date, this sensitivity has been largely characterized using a range of models relying on versions of the Arrhenius equation, which predicts an exponential increase in reaction rate with temperature. However, there is growing evidence from laboratory and field studies that observe nonmonotonic responses of reaction rates to variation in temperature, indicating that Arrhenius is not an appropriate model for quantitatively characterizing temperature sensitivity. Recently, Hobbs et al. (2013) developed macromolecular rate theory (MMRT), which incorporates thermodynamic temperature optima as arising from heat capacity differences between isoenzymes. We applied MMRT to measurements of respiration from soils incubated at different temperatures. These soils were collected from three grassland sites across the U.S. Great Plains and reciprocally transplanted, allowing us to isolate the effects of microbial community type from edaphic factors. We found that microbial community type explained roughly 30% of the variation in the CO2 production rate from the labile C pool but that temperature and soil type were most important in explaining variation in labile and recalcitrant C pool size. For six out of the nine soil × inoculum combinations, MMRT was superior to Arrhenius. The MMRT analysis revealed that microbial communities have distinct heat capacity values and temperature sensitivities sometimes independent of soil type. These results challenge the current paradigm for modeling temperature sensitivity of soil C pools and understanding of microbial enzyme dynamics.

  13. Spatio-temporal variability of soil respiration in a spruce-dominated headwater catchment in western Germany

    NASA Astrophysics Data System (ADS)

    Bossa, A. Y.; Diekkrüger, B.

    2014-08-01

    CO2 production and transport from forest floors is an important component of the carbon cycle and is closely related to the global atmosphere CO2 concentration. If we are to understand the feedback between soil processes and atmospheric CO2, we need to know more about the spatio-temporal variability of this soil respiration under different environmental conditions. In this study, long-term measurements were conducted in a spruce-dominated forest ecosystem in western Germany. Multivariate analysis-based similarities between different measurement sites led to the detection of site clusters along two CO2 emission axes: (1) mainly controlled by soil temperature and moisture condition, and (2) mainly controlled by root biomass and the forest floor litter. The combined effects of soil temperature and soil moisture were used as a time-dependent rating factor affecting the optimal CO2 production and transport at cluster level. High/moderate/weak time-dependent rating factors were associated with the different clusters. The process-based, most distant clusters were identified using specified pattern characteristics: the reaction rates in the soil layers, the activation energy for bio-chemical reactions, the soil moisture dependency parameter, the root biomass factor, the litter layer factor and the organic matter factor. A HYDRUS-1D model system was inversely used to compute soil hydraulic parameters from soil moisture measurements. Heat transport parameters were calibrated based on observed soil temperatures. The results were used to adjust CO2 productions by soil microorganisms and plant roots under optimal conditions for each cluster. Although the uncertainty associated with the HYDRUS-1D simulations is higher, the results were consistent with both the multivariate clustering and the time-dependent rating of site production. Finally, four clusters with significantly different environmental conditions (i.e. permanent high soil moisture condition, accumulated litter amount

  14. Ragweed subpollen particles of respirable size activate human dendritic cells.

    PubMed

    Pazmandi, Kitti; Kumar, Brahma V; Szabo, Krisztina; Boldogh, Istvan; Szoor, Arpad; Vereb, Gyorgy; Veres, Agota; Lanyi, Arpad; Rajnavolgyi, Eva; Bacsi, Attila

    2012-01-01

    Ragweed (Ambrosia artemisiifolia) pollen grains, which are generally considered too large to reach the lower respiratory tract, release subpollen particles (SPPs) of respirable size upon hydration. These SPPs contain allergenic proteins and functional NAD(P)H oxidases. In this study, we examined whether exposure to SPPs initiates the activation of human monocyte-derived dendritic cells (moDCs). We found that treatment with freshly isolated ragweed SPPs increased the intracellular levels of reactive oxygen species (ROS) in moDCs. Phagocytosis of SPPs by moDCs, as demonstrated by confocal laser-scanning microscopy, led to an up-regulation of the cell surface expression of CD40, CD80, CD86, and HLA-DQ and an increase in the production of IL-6, TNF-α, IL-8, and IL-10. Furthermore, SPP-treated moDCs had an increased capacity to stimulate the proliferation of naïve T cells. Co-culture of SPP-treated moDCs with allogeneic CD3(+) pan-T cells resulted in increased secretion of IFN-γ and IL-17 by T cells of both allergic and non-allergic subjects, but induced the production of IL-4 exclusively from the T cells of allergic individuals. Addition of exogenous NADPH further increased, while heat-inactivation or pre-treatment with diphenyleneiodonium (DPI), an inhibitor of NADPH oxidases, strongly diminished, the ability of SPPs to induce phenotypic and functional changes in moDCs, indicating that these processes were mediated, at least partly, by the intrinsic NAD(P)H oxidase activity of SPPs. Collectively, our data suggest that inhaled ragweed SPPs are fully capable of activating dendritic cells (DCs) in the airways and SPPs' NAD(P)H oxidase activity is involved in initiation of adaptive immune responses against innocuous pollen proteins. PMID:23251688

  15. Invasion of a semi-arid shrubland by annual grasses increases autotrophic and heterotrophic soil respiration rates due to altered soil moisture and temperature patterns

    NASA Astrophysics Data System (ADS)

    Mauritz, M.; Hale, I.; Lipson, D.

    2010-12-01

    Shrub <-> grassland conversions are a globally occurring phenomenon altering habitat structure, quality and nutrient cycling. Grasses and shrubs differ in their above and belowground biomass allocation, root architecture, phenology, litter quality and quantity. Conversion affects soil microbial communities, soil moisture and temperature and carbon (C) allocation patterns. However, the effect of conversion on C storage is regionally variable and there is no consistent direction of change. In Southern California invasion by annual grasses is a major threat to native shrub communities and it has been proposed that grass invasion increases NPP and ecosystem C storage (Wolkovich et al, 2009). In order to better understand how this shrub <-> grassland conversion changes ecosystem C storage it is important to understand the partitioning of soil respiration into autotrophic and heterotrophic components. Respiration was measured in plots under shrubs and grasses from February when it was cold and wet to July when it was hot and dry, capturing seasonal transitions in temperature and water availability. Roots were excluded under shrubs and grasses with root exclusion cores to quantify heterotrophic respiration. Using total soil respiration (Rt) = autotrophic respiration (root) (Ra)+ heterotrophic respiration (microbial) (Rh) the components contributing to total soil respiration can be evaluated. Respiration, soil moisture and temperature were measured daily at four hour intervals using Licor 8100 automated chamber measurements. Throughout the measurement period, Rt under grasses exceeded Rt under shrubs. Higher Rt levels under grasses were mainly due to higher Ra in grasses rather than changes in Rh. On average grass Ra was almost double shrub Ra. Higher grass respiration levels are partially explained by differences in soil moisture and temperature between shrubs and grasses. Respiration rates responded similarly to seasonal transitions regardless of treatment although Ra

  16. Effects of experimental soil warming on soil, autotrophic and heterotrophic respirations in cool-temperate deciduous broad-leaved forests

    NASA Astrophysics Data System (ADS)

    Noh, N.; Kuribayashi, M.; Saitoh, T. M.; Nakamura, M.; Nakaji, T.; Hiura, T.; Muraoka, H.

    2013-12-01

    Global warming has the potential to impact on soil respiration (Rs), one of the major fluxes in the global carbon cycle. The different responses of autotrophic (Ra) and heterotrophic (Rh) components of Rs to increasing temperature are expected to have significant consequences for forest ecosystem carbon dynamics. Furthermore, clarification of site-specific difference in their temperature responses is also important for estimating future carbon dynamics in global scale. Here we report the results of open-field soil warming experiments to examine the effects of elevated temperature on the respiration rates in cool-temperate deciduous broad-leaved mature forests in Japan. The experiments were carried out in two JaLTER sites, Takayama in central Japan (TKY, 36○08'N, 137○25'E) and Tomakomai in Hokkaido island of northern Japan (TOEF, 42○40'N, 141○36'E). The dominant tree species (Quercus crispula) and annual mean air temperature (6.5-6.6○C) are similar between the sites. Our objectives were to quantify the effects of soil warming (+3○C in TKY and +4.7○C in TOEF) on the respiration rates, and to determine their sensitivities to given temperature condition. Artificial warming was conducted by installing heating cables into the soil. In addition, to assess how Rs, Ra, and Rh are affected by the treatment differently, we combined the soil warming treatment and trenching treatment in both sites. The warming treatments enhanced annual Rs by 15% (1.2 t C/ha/yr) in TKY and 34% (2.4 t C/ha/yr) in TOEF, and Rh in the first half-year after trenching treatment by 53% in TKY and 52% in TOEF, respectively. Temperature sensitivities of Rs acclimatized to the warming treatment in both sites, while Ra and Rh responded differently to temperature increase between TKY and TOEF. Our results indicate that the responses of those variables to experimental warming differ depending on soil conditions (e.g. soil properties and root distributions) even in the similar forest ecosystem

  17. Understanding environmental drivers in the regulation of soil respiration dynamics after fire in semi-arid ecosystems

    NASA Astrophysics Data System (ADS)

    Muñoz-Rojas, Miriam; Lewandrowski, Wolfgang; Erickson, Todd E.; Dixon, Kingsley W.; Merritt, David J.

    2016-04-01

    Keywords: Pilbara, soil CO2 efflux, soil C, soil moisture, soil temperature Introduction Soil respiration (Rs) has become a major research focus given the increase in atmospheric CO2 emissions and the large contribution of these CO2 fluxes from soils (Van Groenigen et al., 2014). In addition to its importance in the global C cycle, Rs is a fundamental indicator of soil health and quality that reflects the level of microbial activity and provides an indication of the ability of soils to support plant growth (Oyonarte et al., 2012; Munoz-Rojas et al., 2015). Wildfires can have a significant impact on Rs rates, with the scale of the impact depending on environmental factors such as temperature and moisture, and organic C content in the soil. Vegetation cover can have a significant effect on regulating organic C contents; and while advances are made into understanding the effects of fire on organic C contents and CO2 fluxes (Granged et al., 2011; Willaarts et al., 2015; Muñoz-Rojas et al., 2016), there is limited knowledge of the variability of Rs across ecosystem types, vegetation communities, and responses to fire. In this research we aimed to assess the impacts of a wildfire on the soil CO2 fluxes and soil respiration in a semi-arid ecosystem of Western Australia (Pilbara biogeographical region), and to understand the main environmental drivers controlling these fluxes in different vegetation types. The study has application for other arid and semi-arid regions of the world. Methods The study area was selected following a wildfire that affected 25 ha in February 2014. Twelve plots were established in the burnt site (B) within a 400 m2 area, and 12 plots in an adjacent unburnt control site. At each site, three plots were installed below the canopy of each of the most representative vegetation types of the areas: Eucalyptus trees, Acacia shrubs and Triodia grasses, and three on bare soil. Soil sampling and measurement of soil CO2 efflux, temperature and moisture were

  18. Strong resilience of soil respiration components to drought-induced die-off resulting in forest secondary succession.

    PubMed

    Barba, Josep; Curiel Yuste, Jorge; Poyatos, Rafael; Janssens, Ivan A; Lloret, Francisco

    2016-09-01

    How forests cope with drought-induced perturbations and how the dependence of soil respiration on environmental and biological drivers is affected in a warming and drying context are becoming key questions. The aims of this study were to determine whether drought-induced die-off and forest succession were reflected in soil respiration and its components and to determine the influence of climate on the soil respiration components. We used the mesh exclusion method to study seasonal variations in soil respiration (R S) and its components: heterotrophic (R H) and autotrophic (R A) [further split into fine root (R R) and mycorrhizal respiration (R M)] in a mixed Mediterranean forest where Scots pine (Pinus sylvestris L.) is undergoing a drought-induced die-off and is being replaced by holm oak (Quercus ilex L.). Drought-induced pine die-off was not reflected in R S nor in its components, which denotes a high functional resilience of the plant and soil system to pine die-off. However, the succession from Scots pine to holm oak resulted in a reduction of R H and thus in an important decrease of total respiration (R S was 36 % lower in holm oaks than in non-defoliated pines). Furthermore, R S and all its components were strongly regulated by soil water content-and-temperature interaction. Since Scots pine die-off and Quercus species colonization seems to be widely occurring at the driest limit of the Scots pine distribution, the functional resilience of the soil system over die-off and the decrease of R S from Scots pine to holm oak could have direct consequences for the C balance of these ecosystems. PMID:26879544

  19. Temperature Sensitivity and Basal Rate of Soil Respiration and Their Determinants in Temperate Forests of North China

    PubMed Central

    Zhou, Zhiyong; Guo, Chao; Meng, He

    2013-01-01

    The basal respiration rate at 10°C (R10) and the temperature sensitivity of soil respiration (Q10) are two premier parameters in predicting the instantaneous rate of soil respiration at a given temperature. However, the mechanisms underlying the spatial variations in R10 and Q10 are not quite clear. R10 and Q10 were calculated using an exponential function with measured soil respiration and soil temperature for 11 mixed conifer-broadleaved forest stands and nine broadleaved forest stands at a catchment scale. The mean values of R10 were 1.83 µmol CO2 m−2 s−1 and 2.01 µmol CO2 m−2 s−1, the mean values of Q10 were 3.40 and 3.79, respectively, for mixed and broadleaved forest types. Forest type did not influence the two model parameters, but determinants of R10 and Q10 varied between the two forest types. In mixed forest stands, R10 decreased greatly with the ratio of coniferous to broadleaved tree species; whereas it sharply increased with the soil temperature range and the variations in soil organic carbon (SOC), and soil total nitrogen (TN). Q10 was positively correlated with the spatial variances of herb-layer carbon stock and soil bulk density, and negatively with soil C/N ratio. In broadleaved forest stands, R10 was markedly affected by basal area and the variations in shrub carbon stock and soil phosphorus (P) content; the value of Q10 largely depended on soil pH and the variations of SOC and TN. 51% of variations in both R10 and Q10 can be accounted for jointly by five biophysical variables, of which the variation in soil bulk density played an overwhelming role in determining the amplitude of variations in soil basal respiration rates in temperate forests. Overall, it was concluded that soil respiration of temperate forests was largely dependent on soil physical properties when temperature kept quite low. PMID:24339966

  20. Microbial biomass and basal respiration of selected Sub-Antarctic and Antarctic soils in the areas of some Russian polar stations

    NASA Astrophysics Data System (ADS)

    Abakumov, E.; Mukhametova, N.

    2014-07-01

    Antarctica is a unique place for soil, biological, and ecological investigations. Soils of Antarctica have been studied intensively during the last century, when different national Antarctic expeditions visited the sixth continent with the aim of investigating nature and the environment. Antarctic investigations are comprised of field surveys mainly in the terrestrial landscapes, where the polar stations of different countries are situated. That is why the main and most detailed soil surveys were conducted in the McMurdo Valleys, Transantarctic Mountains, South Shetland Islands, Larsemann Hills and the Schirmacher Oasis. Our investigations were conducted during the 53rd and 55th Russian Antarctic expeditions in the base of soil pits, and samples were collected in Sub-Antarctic and Antarctic regions. Sub-Antarctic or maritime landscapes are considered to be very different from Antarctic landscapes due to differing climatic and geogenic conditions. Soils of diverse zonal landscapes were studied with the aim of assessing the microbial biomass level, basal respiration rates and metabolic activity of microbial communities. This investigation shows that Antarctic soils are quite diverse in profile organization and carbon content. In general, Sub-Antarctic soils are characterized by more developed humus (sod) organo-mineral horizons as well as by an upper organic layer. The most developed organic layers were revealed in peat soils of King George Island, where its thickness reach, in some cases, was 80 cm. These soils as well as soils formed under guano are characterized by the highest amount of total organic carbon (TOC), between 7.22 and 33.70%. Coastal and continental Antarctic soils exhibit less developed Leptosols, Gleysols, Regolith and rare Ornhitosol, with TOC levels between 0.37 and 4.67%. The metabolic ratios and basal respiration were higher in Sub-Antarctic soils than in Antarctic ones, which can be interpreted as a result of higher amounts of fresh organic

  1. Spatio-temporal variability of soil respiration in a spruce-dominated headwater catchment in western Germany

    NASA Astrophysics Data System (ADS)

    Bossa, A. Y.; Diekkrüger, B.

    2014-01-01

    CO2 production and transport from forest floors is an important component of the carbon cycle and is closely related to the global atmosphere CO2 concentration. If we are to understand the feedback between soil processes and atmospheric CO2, we need to know more about the spatio-temporal variability of this soil respiration under different environmental conditions. In this study, long-term measurements were conducted in a spruce-dominated forest ecosystem in western Germany. Multivariate analysis-based similarities between different measurements sites led to the detection of site clusters along two CO2 emission axes: (1) mainly controlled by soil temperature and moisture condition, and (2) mainly controlled by root biomass and the forest floor litter. The combined effects of soil temperature and soil moisture were used as a time-dependent rating factor affecting the optimal CO2 production and transport at cluster level. High/moderate/weak time-dependent rating factors were associated with the different clusters. The process-based most distant clusters were identified using specified pattern characteristics: the reaction rates in the soil layers, the activation energy for bio-chemical reactions, the water sorption and desorption constant, the root biomass factor, the litter layer factor and the organic matter factor. A HYDRUS-1D model system was inversely used to compute soil hydraulic parameters from soil moisture measurements. Heat transport parameters were adjusted based on observed soil temperatures. The results were used to adjust CO2 production and transport characteristics such as the molecular diffusion coefficient of carbon dioxide in air and water and the CO2 production by soil microorganisms and plant roots under optimal conditions for each cluster. Although the uncertainty associated with the HYDRUS-1-D simulations is higher, the results were consistent with both the multivariate clustering and the time-dependent rating of site production

  2. Basal respiration and composition of microbial biomass in virgin and agroforest-reclaimed semidesert soils of the Northern Caspian region

    NASA Astrophysics Data System (ADS)

    Prikhod'ko, V. E.; Sizemskaya, M. L.

    2015-08-01

    Virgin semidesert soils and their analogues subjected to agroforest reclamation 60 years ago were studied in the area of the Dzhanybek Research Station of the Institute of Forest Science of the Russian Academy of Sciences in the Northern Caspian region. The values of Cmic and soil basal respiration (BR) significantly vary among the separate plots. In the 0- to 10-cm layer, the BR rate is 0.28-2.44 μg C-CO2/(g h); the minimum values are typical for the arable soils of the interbelt area, and the maximum values are found in the meadow-chestnut soils of mesodepressions under the forest belt with a strong zoogenic effect. The content of Cmic increases from 415 to 1388 μg C/g soil in the following series: virgin solonetz-agro-afforested soils- virgin meadow-chestnut soils = their forest analogues. The fungi/bacteria ratio is 1.3-3.0; the fungal component of soils reaches 53-85% of Cmic, and its absolute values increase from 236 to 1040 μg C/g in the same soil series. Correlation was found between Corg and BR ( r = 0.89), between Corg and Cmic ( r = 0.87), and between BR and Cmic ( r = 0.89). The portion of Cmic in Corg is 3.2-8.6%; the minimum values are found for virgin solonetz and meadow-chestnut soil under forest belt with strong zoogenic effect. The values of qCO2 (ratio of BR to Cmic) are in the range of 0.7-2 μg C-CO2/(mg Cmic g h). At the afforestation of soils in natural and artificial mesodepressions, the activation of microbial community and humification processes is noted compared to the virgin analogues; unstable microbiological processes and a decreased Corg content because of deep tillage and the reduced input of plant residues in the permanent bare fallow between forest belts are revealed in the agro-afforested solonetzes and meadow-chestnut soils of microdepressions.

  3. In Situ Contribution of Old Respired CO2 from Soils in Burnt and Collapsed Permafrost in Canada

    NASA Astrophysics Data System (ADS)

    Estop-Aragones, C.; Fisher, J. P.; Cooper, M.; Thierry, A.; Williams, M. D.; Phoenix, G. K.; Murton, J.; Charman, D.; Hartley, I. P.

    2014-12-01

    Permafrost degradation is associated with an aggradation of the active layer thus exposing previously frozen soil carbon (C) to microbial activity. This may increase the generation of greenhouse gases and potentially increase rates of climate change. However, the rate of C release remains highly uncertain, not least because few in situ studies have measured the rate at which previously frozen C is released from the soil surface, post thaw. We quantified the contribution of this "old" C being released as CO2 from permafrost degraded soils in sporadic and discontinuous permafrost in Yukon and Northwest Territories, Canada. Firstly, we studied the effect of fire on black spruce forests as the removal of vegetation, especially mosses, may play a key role on thaw depth. Secondly, we investigated the collapse of peatland plateau after permafrost thaw which resulted in the formation of wetlands. We combined radiocarbon measurements of respired CO2 with a novel collar-design that either included or excluded CO2 released from deeper soil horizons. Our results show that, while excluding deeper layers did reduce the average age of the C being released from the soil surface, more than 90% of the CO2 came from contemporary sources, even after burnt and permafrost plateau collapse. Furthermore, soil cores dated using 210Pb show that the rapid accumulation of sedge peat after plateau collapse may more than compensate for any C losses from depth. Our results from the Canadian boreal contrast strongly with findings from other geographical areas emphasising the complexities of predicting the impact of permafrost thaw on the carbon balance of northern ecosystems.

  4. Respiration testing for bioventing and biosparging remediation of petroleum contaminated soil and ground water

    SciTech Connect

    Gray, A.L.; Brown, A.; Moore, B.J.; Payne, R.E.

    1996-12-01

    Respiration tests were performed to measure the effect of subsurface aeration on the biodegradation rates of petroleum hydrocarbon contamination in vadose zone soils (bioventing) and ground water (biosparging). The aerobic biodegradation of petroleum contamination is typically limited by the absence of oxygen in the soil and ground water. Therefore, the goal of these bioremediation technologies is to increase the oxygen concentration in the subsurface and thereby enhance the natural aerobic biodegradation of the organic contamination. One case study for biosparging bioremediation testing is presented. At this site atmospheric air was injected into the ground water to increase the dissolved oxygen concentration in the ground water surrounding a well, and to aerate the smear zone above the ground water table. Aeration flow rates of 3 to 8 cfm (0.09 to 0.23 m{sup 3}/min) were sufficient to increase the dissolved oxygen concentration. Petroleum hydrocarbon biodegradation rates of 32 to 47 {micro}g/l/hour were calculated based on measurements of dissolved oxygen concentration in ground water. The results of this test have demonstrated that biosparging enhances the biodegradation of petroleum hydrocarbons, but the results as they apply to remediation are not known. Two case studies for bioventing respiration testing are presented.

  5. Heterotrophic Soil Respiration in Warming Experiments: Using Microbial Indicators to Partition Contributions from Labile and Recalcitrant Soil Organic Carbon. Final Report

    SciTech Connect

    Bradford, M A; Melillo, J M; Reynolds, J F; Treseder, K K; Wallenstein, M D

    2010-06-10

    The central objective of the proposed work was to develop a genomic approach (nucleic acid-based) that elucidates the mechanistic basis for the observed impacts of experimental soil warming on forest soil respiration. The need to understand the mechanistic basis arises from the importance of such information for developing effective adaptation strategies for dealing with projected climate change. Specifically, robust predictions of future climate will permit the tailoring of the most effective adaptation efforts. And one of the greatest uncertainties in current global climate models is whether there will be a net loss of carbon from soils to the atmosphere as climate warms. Given that soils contain approximately 2.5 times as much carbon as the atmosphere, a net loss could lead to runaway climate warming. Indeed, most ecosystem models predict that climate warming will stimulate microbial decomposition of soil carbon, producing such a positive feedback to rising global temperatures. Yet the IPCC highlights the uncertainty regarding this projected feedback. The uncertainty arises because although warming-experiments document an initial increase in the loss of carbon from soils, the increase in respiration is short-lived, declining to control levels in a few years. This attenuation could result from changes in microbial physiology with temperature. We explored possible microbial responses to warming using experiments and modeling. Our work advances our understanding of how soil microbial communities and their activities are structured, generating insight into how soil carbon might respond to warming. We show the importance of resource partitioning in structuring microbial communities. Specifically, we quantified the relative abundance of fungal taxa that proliferated following the addition of organic substrates to soil. We added glycine, sucrose, cellulose, lignin, or tannin-protein to soils in conjunction with 3-bromo-deoxyuridine (BrdU), a nucleotide analog. Active

  6. Unifying soil respiration pulses, inhibition, and temperature hysteresis through dynamics of labile soil carbon and O2

    NASA Astrophysics Data System (ADS)

    Oikawa, P. Y.; Grantz, D. A.; Chatterjee, A.; Eberwein, J. E.; Allsman, L. A.; Jenerette, G. D.

    2014-04-01

    Event-driven and diel dynamics of soil respiration (Rs) strongly influence terrestrial carbon (C) emissions and are difficult to predict. Wetting events may cause a large pulse or strong inhibition of Rs. Complex diel dynamics include hysteresis in the relationship between Rs and soil temperature. The mechanistic basis for these dynamics is not well understood, resulting in large discrepancies between predicted and observed Rs. We present a unifying approach for interpreting these phenomena in a hot arid agricultural environment. We performed a whole ecosystem wetting experiment with continuous measurement of Rs to study pulse responses to wetting in a heterotrophic system. We also investigated Rs during cultivation of Sorghum bicolor to evaluate the role of photosynthetic C in the regulation of diel variation in Rs. Finally, we adapted a Rs model with sensitivity to soil O2 and water content by incorporating two soil C pools differing in lability. We observed a large wetting-induced pulse of Rs from the fallow field and were able to accurately simulate the pulse via release of labile soil C. During the exponential phase of plant growth, Rs was inhibited in response to wetting, which was accurately simulated through depletion of soil O2. Without plants, hysteresis was not observed; however, with growing plants, an increasingly significant counterclockwise hysteresis developed. Hysteresis was simulated via a dynamic photosynthetic C pool and was not likely controlled by physical processes. These results help characterize the complex regulation of Rs and improve understanding of these phenomena under warmer and more variable conditions.

  7. Biological soil crusts are the main contributor to winter soil respiration in a temperate desert ecosystem of China

    NASA Astrophysics Data System (ADS)

    He, M. Z.

    2012-04-01

    Aims Biological soil crusts (BSCs) are a key biotic component of desert ecosystems worldwide. However, most studies carried out to date on carbon (fluxes) in these ecosystems, such as soil respiration (RS), have neglected them. Also, winter RS is reported to be a significant component of annual carbon budget in other ecosystems, however, we have less knowledge about winter RS of BSCs in winter and its contribution to carbon cycle in desert regions. Therefore, the specific objectives of this study were to: (i) quantify the effects of different BSCs types (moss crust, algae crust, physical crust) on the winter RS; (ii) explore relationships of RS against soil temperature and water content for different BSCs, and (iii) assess the relative contribution of BSCs to the annual amount of C released by RS at desert ecosystem level. Methods Site Description The study sites are located at the southeast fringe of the Tengger Desert in the Shapotou region of the Ningxia Hui Autonomous Region [37°32'N and 105°02'E, at 1340 m above mean sea level (a.m.s.l.)], western China. The mean daily temperature in January is -6.9°C , while it is 24.3°C in July. The mean annual precipitation is 186 mm, approximately 80% of which falls between May and September. The annual potential evaporation is 2800 mm. The landscape of the Shapotou region is characterized by large and dense reticulate barchans chains of sand dunes that migrate south-eastward at a velocity of 3-6 m per year. The soil is loose, infertile and mobile and can thus be classified as orthic sierozem and Aeolian sandy soil. Additionally, the soil has a consistent gravimetric water content that ranges from 3 to 4%. The groundwater in the study area is too deep (>60 m) to support large areas of the native vegetation cover; therefore, precipitation is usually the only source of freshwater. The predominant native plants are Hedysarum scoparium Fisch. and Agriophyllum squarrosum Moq., Psammochloa cillosa Bor, which scattered

  8. The diel imprint of leaf metabolism on the δ13 C signal of soil respiration under control and drought conditions.

    PubMed

    Barthel, Matthias; Hammerle, Albin; Sturm, Patrick; Baur, Thomas; Gentsch, Lydia; Knohl, Alexander

    2011-12-01

    Recent (13) CO(2) canopy pulse chase labeling studies revealed that photosynthesis influences the carbon isotopic composition of soil respired CO(2) (δ(13) C(SR)) even on a diel timescale. However, the driving mechanisms underlying these short-term responses remain unclear, in particular under drought conditions. The gas exchange of CO(2) isotopes of canopy and soil was monitored in drought/nondrought-stressed beech (Fagus sylvatica) saplings after (13) CO(2) canopy pulse labeling. A combined canopy/soil chamber system with gas-tight separated soil and canopy compartments was coupled to a laser spectrometer measuring mixing ratios and isotopic composition of CO(2) in air at high temporal resolution. The measured δ(13) C(SR) signal was then explained and substantiated by a mechanistic carbon allocation model. Leaf metabolism had a strong imprint on diel cycles in control plants, as a result of an alternating substrate supply switching between sugar and transient starch. By contrast, diel cycles in drought-stressed plants were determined by the relative contributions of autotrophic and heterotrophic respiration throughout the day. Drought reduced the speed of the link between photosynthesis and soil respiration by a factor of c. 2.5, depending on the photosynthetic rate. Drought slows the coupling between photosynthesis and soil respiration and alters the underlying mechanism causing diel variations of δ(13) C(SR). PMID:21851360

  9. Distinct patterns in the diurnal and seasonal variability in four components of soil respiration in a temperate forest under free-air CO2 enrichment

    NASA Astrophysics Data System (ADS)

    Taneva, L.; Gonzalez-Meler, M. A.

    2011-10-01

    explained by only temperature and moisture variations. Our results indicate that the variation observed in the components of RS is the result of complex interaction between dominant biotic controls (e.g. plant activity, mineralization kinetics, competition for substrates) over abiotic controls (temperature, moisture). The interactions and controls among roots and other soil organisms that utilize C of different chemistry, accessibility and ages, results in the overall soil CO2 efflux. Therefore understanding the controls on the components of RS is necessary to elucidate the influence of ecosystem respiration on atmospheric C-pools at different time scales.

  10. [Temporal variation of soil respiration on sloping pasture of Heihe River basin and effects of temperature and soil moisture on it].

    PubMed

    Chang, Zongqiang; Shi, Zuomin; Feng, Qi; Su, Yonghong

    2005-09-01

    Employing LiCor 6400 gas exchange analyzer and soil respiration chamber attachment (LiCor Inc., Lincoln, NE, USA), this paper continuously measured the soil surface CO2 effluxes on the sloping pasture of Heihe River basin from early April to late October 2003 to investigate the soil CO2 efflux rate and its feedback to the changes of climate and land use. The results showed that from May to October, the diurnal variation of soil respiration was low at night, the lowest at 7:00, 6:30, 5:30, 6:00 and 7:00, raised rapidly at 7:00 - 8:30, and then descended at 16:00 - 18:30. The maximum soil CO2 efflux appeared at 15:00, 14:30, 14:30, 13:30, 14:00 and 15:00. The mean daily soil respiration rate was 0.31 - 6.98 micromol m(-2) s(-1), with the maximum in July and August, the second in May and September, and nearly consistent in April and October. Soil respiration rate had an exponential and power correlation with temperature and soil moisture, respectively. PMID:16358421

  11. Soil respiration shifts as drought-induced tree substitution advances from Scots pine to Holm oak forest

    NASA Astrophysics Data System (ADS)

    Barba, Josep; Curiel Yuste, Jorge; Poyatos, Rafael; Janssens, Ivan A.; Lloret, Francisco

    2014-05-01

    There is more and more evidences that the current global warming trend and the increase of frequency and intensity of drought events during the last decades in the Northern hemisphere are currently producing an increment of drought-induced forest die-off events, being the Mediterranean region one of the most affected areas. This drought-induced mortality could lead in a vegetation shift with unpredicted consequences in carbon pools, where soils are the most determinant factor in this carbon balance as they contain over two-thirds of carbon on forest ecosystems. There are several uncertainties related on the interaction between soil, environmental conditions and vegetation shifts that could modify their capability to be net carbon sinks or sources in a warming context. We studied soil respiration and its heterotrophic (RH) and autotrophic (Ra) (split in fine roots [Rr] and mycorrhizal respiration [Rs]) components in a mixed Mediterranean forest where Scots pine (Pinus sylvestris L.) are suffering from drought-induced die-off and replaced by Holm oak (Quercus ilex L.) as the dominant tree species. Soil respiration fluxes and its fractions were measured every two weeks during one year at four stages of the substitution process (non defoliated pines [NDP], defoliated pines [DFP], dead pines [DP] and Holm oak [HO]), using the mesh exclusion method. The aims were (i) to describe soil respiration fluxes in a drought-induced secondary successional process, (ii) to test whether the changes in vegetation affected soil respiration fluxes and (iii) to determine the influence of environmental and abiotic variables on the different soil respiration fractions. Total soil respiration was 10.10±6.17 TC ha-1 y-1, RH represented the 67% of the total, Ra represented the 34% of the total, and Rr and Rs were the 22 and 12%, respectively. Significant differences were found in total soil respiration and RH between NDP and HO, being lower in HO than in NDP (34% in total and 48% in RH). No

  12. Shifting resource availability, plastic allocation to exoenzymes and the consequences for heterotrophic soil respiration

    NASA Astrophysics Data System (ADS)

    Ballantyne, Ford; Billings, Sharon

    2014-05-01

    The rate of decomposition of soil organic matter (SOM) is influenced by the availability of substrates in the soil matrix, the chemical composition of organic matters substrates, and the reaction kinetics of exoenzymes secreted by microbes. Predicting carbon (C) flow from SOM into respired CO2 is predicated on knowledge of feedbacks between substrate availability and microbial resource allocation. It is critical to understand physiological responses of microbes to their environments because it is the feedbacks between the abiotic conditions and resource availability that govern exoenzyme synthesis. Without mechanistic knowledge, it is difficult to project how warming and changing edaphic characteristics will influence respiratory CO2 losses from soils. Here, we apply a general theoretical framework that describes the consequences of interactions between exoenzymes, SOM substrates, microbial resource allocation and microbial stoichiometry to explore how different edaphic conditions give rise to different microbial niches. Our approach incorporates the kinetics of exoenzyme-substrate interactions, the costs and benefits associated with producing different exoenzymes, regulation of biomass C:N, and substrate availability in the soil matrix. We explore how shifting resource availability forces microbes to alter their strategies for synthesizing exoenzymes to promote acquisition of C and N that satisfies demand. In particular, we study how changing relative C and N availability constrain the degree to biomass C:N can be maintained with plastic allocation to different exoenzymes. Using reaction rate data from purified enzyme-substrate experiments, we conclude that shifts in both the absolute and relative availability of substrates with different C:N give rise to clear niches in C and N allocation space. These niches correspond to environments that are typically associated with soil microbes exhibiting different biomass C:N. Finally, we show that the allocation changes

  13. Effect of moisture on leaf litter decomposition and its contribution to soil respiration in a temperate forest

    NASA Astrophysics Data System (ADS)

    Cisneros-Dozal, Luz Maria; Trumbore, Susan E.; Hanson, Paul J.

    2007-03-01

    The degree to which increased soil respiration rates following wetting is caused by plant (autotrophic) versus microbial (heterotrophic) processes, is still largely uninvestigated. Incubation studies suggest microbial processes play a role but it remains unclear whether there is a stimulation of the microbial population as a whole or an increase in the importance of specific substrates that become available with wetting of the soil. We took advantage of an ongoing manipulation of leaf litter 14C contents at the Oak Ridge Reservation, Oak Ridge, Tennessee, to (1) determine the degree to which an increase in soil respiration rates that accompanied wetting of litter and soil, following a short period of drought, could be explained by heterotrophic contributions; and (2) investigate the potential causes of increased heterotrophic respiration in incubated litter and 0-5 cm mineral soil. The contribution of leaf litter decomposition increased from 6 ± 3 mg C m-2 hr-1 during a transient drought, to 63 ± 18 mg C m-2 hr-1 immediately after water addition, corresponding to an increase in the contribution to soil respiration from 5 ± 2% to 37 ± 8%. The increased relative contribution was sufficient to explain all of the observed increase in soil respiration for this one wetting event in the late growing season. Temperature (13°C versus 25°C) and moisture (dry versus field capacity) conditions did not change the relative contributions of different decomposition substrates in incubations, suggesting that more slowly cycling C has at least the same sensitivity to decomposition as faster cycling organic C at the temperature and moisture conditions studied.

  14. Effect of moisture on leaf litter decomposition and its contribution to soil respiration in a temperate forest

    SciTech Connect

    Cisneros-Dozal, Luz Maria; Trumbore, Susan E.; Hanson, Paul J

    2007-01-01

    The degree to which increased soil respiration rates following wetting is caused by plant (autotrophic) versus microbial (heterotrophic) processes, is still largely uninvestigated. Incubation studies suggest microbial processes play a role but it remains unclear whether there is a stimulation of the microbial population as a whole or an increase in the importance of specific substrates that become available with wetting of the soil. We took advantage of an ongoing manipulation of leaf litter 14C contents at the Oak Ridge Reservation, Oak Ridge, Tennessee, to (1) determine the degree to which an increase in soil respiration rates that accompanied wetting of litter and soil, following a short period of drought, could be explained by heterotrophic contributions; and (2) investigate the potential causes of increased heterotrophic respiration in incubated litter and 0-5 cm mineral soil. The contribution of leaf litter decomposition increased from 6 3 mg C m 2 hr 1 during a transient drought, to 63 18 mg C m 2 hr 1 immediately after water addition, corresponding to an increase in the contribution to soil respiration from 5 2% to 37 8%. The increased relative contribution was sufficient to explain all of the observed increase in soil respiration for this one wetting event in the late growing season. Temperature (13 C versus 25 C) and moisture (dry versus field capacity) conditions did not change the relative contributions of different decomposition substrates in incubations, suggesting that more slowly cycling C has at least the same sensitivity to decomposition as faster cycling organic C at the temperature and moisture conditions studied.

  15. Not so hot: Rapid recovery of soil temperature and respiration following tornado damage, regardless of disturbance severity

    NASA Astrophysics Data System (ADS)

    Nagendra, U.; Peterson, C.

    2013-12-01

    Forest disturbances such as tornadoes are expected to raise soil temperatures and increase soil respiration. Opening canopy gaps allows solar radiation to heat the forest floor, and damaged plant roots provide fuel for decomposition. Patches of disturbed forest can range from low severity (some defoliation, broken branches) to high severity (uprooted or snapped trees). Disturbance severity affects plant population and community processes, such as regeneration mode, species diversity, and community structure. We expect disturbance severity to also affect ecosystem processes such as soil respiration. Severe disturbances cause more distinct, and often larger, canopy gaps than mild disturbances, and damage more standing biomass, both above- and below-ground. We would expect these larger gaps and greater litter amounts to increase soil temperature and respiration in more severely disturbed forest patches. In April 2011, a moderate (EF-3) tornado damaged portions of the Chattahoochee National Forest in NE Georgia, USA. Our lab has been characterizing the damage and regeneration in sections of the forest since summer 2011. In Spring 2013, we installed 4 iButton temperature sensors in each of 14 plots across a range of disturbance severity (for a total of 56 sensors). Severity was determined by percent of initial tree basal area downed by the tornado, and ranged from 8% to 100% basal area down. The iButtons monitored soil temperature at a depth of 5 cm every hour for 85 days. In July 2013, integrated 24-hour soil respiration was measured at the same locations using soda lime absorption in sealed PVC collars. Soil temperature at 5 cm averaged 12.66 °C. Contrary to expectations, average daily temperatures did not increase with greater plot damage severity (R2 = 0.001). Daily variation was only slightly higher in plots of very high severity. Overall, soil temperatures appeared to have returned to pre-disturbance temperatures more quickly than expected. Results for upcoming

  16. Learning about Cellular Respiration: An Active Approach Illustrating the Process of Scientific Inquiry.

    ERIC Educational Resources Information Center

    Johnson, Margaret (Peg)

    1998-01-01

    Details the active-learning approach to teaching cellular respiration in an introductory, one-semester course for nonmajors. Focuses on a laboratory exercise designed to answer the question of what happens to food when eaten. Contains 19 references. (DDR)

  17. An instrument design and sample strategy for measuring soil respiration in the coastal temperate rain forest

    NASA Astrophysics Data System (ADS)

    Nay, S. M.; D'Amore, D. V.

    2009-12-01

    The coastal temperate rainforest (CTR) along the northwest coast of North America is a large and complex mosaic of forests and wetlands located on an undulating terrain ranging from sea level to thousands of meters in elevation. This biome stores a dynamic portion of the total carbon stock of North America. The fate of the terrestrial carbon stock is of concern due to the potential for mobilization and export of this store to both the atmosphere as carbon respiration flux and ocean as dissolved organic and inorganic carbon flux. Soil respiration is the largest export vector in the system and must be accurately measured to gain any comprehensive understanding of how carbon moves though this system. Suitable monitoring tools capable of measuring carbon fluxes at small spatial scales are essential for our understanding of carbon dynamics at larger spatial scales within this complex assemblage of ecosystems. We have adapted instrumentation and developed a sampling strategy for optimizing replication of soil respiration measurements to quantify differences among spatially complex landscape units of the CTR. We start with the design of the instrument to ease the technological, ergonomic and financial barriers that technicians encounter in monitoring the efflux of CO2 from the soil. Our sampling strategy optimizes the physical efforts of the field work and manages for the high variation of flux measurements encountered in this difficult environment of rough terrain, dense vegetation and wet climate. Our soil respirometer incorporates an infra-red gas analyzer (LiCor Inc. LI-820) and an 8300 cm3 soil respiration chamber; the device is durable, lightweight, easy to operate and can be built for under $5000 per unit. The modest unit price allows for a multiple unit fleet to be deployed and operated in an intensive field monitoring campaign. We use a large 346 cm2 collar to accommodate as much micro spatial variation as feasible and to facilitate repeated measures for tracking

  18. Application of mesotrione at different doses in an amended soil: Dissipation and effect on the soil microbial biomass and activity.

    PubMed

    Pose-Juan, Eva; Sánchez-Martín, María Jesús; Herrero-Hernández, Eliseo; Rodríguez-Cruz, María Sonia

    2015-12-01

    The aim of this work was to estimate the dissipation of mesotrione applied at three doses (2, 10 and 50 mg kg(-1) dw) in an unamended agricultural soil, and this same soil amended with two organic residues (green compost (C) and sewage sludge (SS)). The effects of herbicide and organic residue on the abundance and activity of soil microbial communities were also assessed by determining soil microbial parameters such as biomass, dehydrogenase activity (DHA), and respiration. Lower dissipation rates were observed for a higher herbicide dose. The highest half-life (DT50) values were observed in the SS-amended soil for the three herbicide doses applied. Biomass values increased in the amended soils compared to the unamended one in all the cases studied, and increased over the incubation period in the SS-amended soil. DHA mean values significantly decreased in the SS-amended soil, and increased in the C-amended soil compared to the unamended ones, under all conditions. At time 0 days, respiration values were significantly higher in SS-amended soils (untreated and treated with mesotrione) than in the unamended and C-amended soils. The effect of mesotrione on soil biomass, DHA and respiration was different depending on incubation time and soil amendment and herbicide dose applied. The results support the need to consider the possible non-target effects of pesticides and organic amendments simultaneously applied on soil microbial communities to prevent negative impacts on soil quality. PMID:26188530

  19. A cross-biome synthesis of soil respiration and its determinants under simulated precipitation changes.

    PubMed

    Liu, Lingli; Wang, Xin; Lajeunesse, Marc J; Miao, Guofang; Piao, Shilong; Wan, Shiqiang; Wu, Yuxin; Wang, Zhenhua; Yang, Sen; Li, Ping; Deng, Meifeng

    2016-04-01

    Soil respiration (Rs) is the second-largest terrestrial carbon (C) flux. Although Rs has been extensively studied across a broad range of biomes, there is surprisingly little consensus on how the spatiotemporal patterns of Rs will be altered in a warming climate with changing precipitation regimes. Here, we present a global synthesis Rs data from studies that have manipulated precipitation in the field by collating studies from 113 increased precipitation treatments, 91 decreased precipitation treatments, and 14 prolonged drought treatments. Our meta-analysis indicated that when the increased precipitation treatments were normalized to 28% above the ambient level, the soil moisture, Rs, and the temperature sensitivity (Q10) values increased by an average of 17%, 16%, and 6%, respectively, and the soil temperature decreased by -1.3%. The greatest increases in Rs and Q10 were observed in arid areas, and the stimulation rates decreased with increases in climate humidity. When the decreased precipitation treatments were normalized to 28% below the ambient level, the soil moisture and Rs values decreased by an average of -14% and -17%, respectively, and the soil temperature and Q10 values were not altered. The reductions in soil moisture tended to be greater in more humid areas. Prolonged drought without alterations in the amount of precipitation reduced the soil moisture and Rs by -12% and -6%, respectively, but did not alter Q10. Overall, our synthesis suggests that soil moisture and Rs tend to be more sensitive to increased precipitation in more arid areas and more responsive to decreased precipitation in more humid areas. The responses of Rs and Q10 were predominantly driven by precipitation-induced changes in the soil moisture, whereas changes in the soil temperature had limited impacts. Finally, our synthesis of prolonged drought experiments also emphasizes the importance of the timing and frequency of precipitation events on ecosystem C cycles. Given these

  20. Nitrogen Addition Altered the Effect of Belowground C Allocation on Soil Respiration in a Subtropical Forest.

    PubMed

    He, Tongxin; Wang, Qingkui; Wang, Silong; Zhang, Fangyue

    2016-01-01

    The availabilities of carbon (C) and nitrogen (N) in soil play an important role in soil carbon dioxide (CO2) emission. However, the variation in the soil respiration (Rs) and response of microbial community to the combined changes in belowground C and N inputs in forest ecosystems are not yet fully understood. Stem girdling and N addition were performed in this study to evaluate the effects of C supply and N availability on Rs and soil microbial community in a subtropical forest. The trees were girdled on 1 July 2012. Rs was monitored from July 2012 to November 2013, and soil microbial community composition was also examined by phospholipid fatty acids (PLFAs) 1 year after girdling. Results showed that Rs decreased by 40.5% with girdling alone, but N addition only did not change Rs. Interestingly, Rs decreased by 62.7% under the girdling with N addition treatment. The reducing effect of girdling and N addition on Rs differed between dormant and growing seasons. Girdling alone reduced Rs by 33.9% in the dormant season and 54.8% in the growing season compared with the control. By contrast, girdling with N addition decreased Rs by 59.5% in the dormant season and 65.4% in the growing season. Girdling and N addition significantly decreased the total and bacterial PLFAs. Moreover, the effect of N addition was greater than girdling. Both girdling and N addition treatments separated the microbial groups on the basis of the first principal component through principal component analysis compared with control. This indicated that girdling and N addition changed the soil microbial community composition. However, the effect of girdling with N addition treatment separated the microbial groups on the basis of the second principal component compared to N addition treatment, which suggested N addition altered the effect of girdling on soil microbial community composition. These results suggest that the increase in soil N availability by N deposition alters the effect of

  1. Nitrogen Addition Altered the Effect of Belowground C Allocation on Soil Respiration in a Subtropical Forest

    PubMed Central

    He, Tongxin; Wang, Qingkui; Wang, Silong; Zhang, Fangyue

    2016-01-01

    The availabilities of carbon (C) and nitrogen (N) in soil play an important role in soil carbon dioxide (CO2) emission. However, the variation in the soil respiration (Rs) and response of microbial community to the combined changes in belowground C and N inputs in forest ecosystems are not yet fully understood. Stem girdling and N addition were performed in this study to evaluate the effects of C supply and N availability on Rs and soil microbial community in a subtropical forest. The trees were girdled on 1 July 2012. Rs was monitored from July 2012 to November 2013, and soil microbial community composition was also examined by phospholipid fatty acids (PLFAs) 1 year after girdling. Results showed that Rs decreased by 40.5% with girdling alone, but N addition only did not change Rs. Interestingly, Rs decreased by 62.7% under the girdling with N addition treatment. The reducing effect of girdling and N addition on Rs differed between dormant and growing seasons. Girdling alone reduced Rs by 33.9% in the dormant season and 54.8% in the growing season compared with the control. By contrast, girdling with N addition decreased Rs by 59.5% in the dormant season and 65.4% in the growing season. Girdling and N addition significantly decreased the total and bacterial PLFAs. Moreover, the effect of N addition was greater than girdling. Both girdling and N addition treatments separated the microbial groups on the basis of the first principal component through principal component analysis compared with control. This indicated that girdling and N addition changed the soil microbial community composition. However, the effect of girdling with N addition treatment separated the microbial groups on the basis of the second principal component compared to N addition treatment, which suggested N addition altered the effect of girdling on soil microbial community composition. These results suggest that the increase in soil N availability by N deposition alters the effect of

  2. Comparison of soil microbial respiration and carbon turnover under perennial and annual biofuel crops in two agricultural soils

    NASA Astrophysics Data System (ADS)

    Szymanski, L. M.; Marin-Spiotta, E.; Sanford, G. R.; Jackson, R. D.; Heckman, K. A.

    2015-12-01

    Bioenergy crops have the potential to provide a low carbon-intensive alternative to fossil fuels. More than a century of agricultural research has shown that conventional cropping systems can reduce soil organic matter (SOM) reservoirs, which cause long-term soil nutrient loss and C release to the atmosphere. In the face of climate change and other human disruptions to biogeochemical cycles, identifying biofuel crops that can maintain or enhance soil resources is desirable for the sustainable production of bioenergy. The objective of our study was to compare the effects of four biofuel crop treatments on SOM dynamics in two agricultural soils: Mollisols at Arlington Agricultural Research Station in Wisconsin and Alfisols at Kellogg Biological Station in Michigan, USA. We used fresh soils collected in 2013 and archived soils from 2008 to measure the effects of five years of crop management. Using a one-year long laboratory soil incubation coupled with a regression model and radiocarbon measurements, we separated soils into three SOM pools and their corresponding C turnover times. We found that the active pool, or biologically available C, was more sensitive to management and is an earlier indicator of changes to soil C dynamics than bulk soil C measurements. There was no effect of treatment on the active pool size at either site; however, the percent C in the active pool decreased, regardless of crop type, in surface soils with high clay content. At depth, the response of the slow pool differed between annual and perennial cropping systems. The distribution of C among SOM fractions varied between the two soil types, with greater C content associated with the active fraction in the coarser textured-soil and greater C content associated with the slow-cycling fraction in the soils with high clay content. These results suggest that the effects of bioenergy crops on soil resources will vary geographically, with implications for the carbon-cost of biocrop production.

  3. Forest age stands affect soil respiration and litterfall in a Black pine forest managed by a shelterwood system in the Central Spain?

    NASA Astrophysics Data System (ADS)

    Hedo de Santiago, Javier; Borja, Manuel Esteban Lucas; Candel, David; Viñegla Pérez, Benjamin

    2016-04-01

    This study aims to investigate the effects that stand age and forest structure generates on soil respiration and litterfall quantity. The effect of stand age on these variables was studied in a shelterwood system Spanish Black pine chronosequence in central Iberian Peninsula composed of 0-20, 20-40, 40-60, 60-80, 80-100-year-old. For each stand age, six forest stands with similar characteristics of soil type and site preparation were used. Also, a forest area ranging 80-120 years old and without forest intervention was selected and used as control. We also measured organic matter, C:N ratio, soil moisture and pH in the top 10 mineral soil at each compartment. Soil respiration measurements were carried out in three time points (3, 8 and 12 days). Results showed a clear trend in soil respiration, comparing all the experimental areas. Soil respiration showed the same trend in all stands. It initially showed higher rates, reaching stability in the middle of the measurement process and finally lightly increasing the respiration rate. The older stands had significantly higher soil respiration than the younger stands. Soil organic matter values were also higher in the more mature stands. C:N ratio showed the opposite trend, showing lower values in the less mature stands. More mature stands clearly showed more quantity of litterfall than the younger ones and there was a positive correlation between soil respiration and litterfall. Finally, the multivariate PCA analysis clearly clustered three differenced groups: Control plot; from 100 to 40 years old and from 39 to 1 years old, taking into account both soil respiration and litterfall quantity, also separately. Our results suggest that the control plot has a better soil quality and that extreme forest stand ages (100-80 and 19-1 years old) and the associated forest structure generates differences in soil respiration.

  4. The influence of soils on heterotrophic respiration exerts a strong control on net ecosystem productivity in seasonally dry Amazonian forests

    NASA Astrophysics Data System (ADS)

    Melton, J. R.; Shrestha, R. K.; Arora, V. K.

    2014-08-01

    Net ecosystem productivity of carbon (NEP) in seasonally dry forests of the Amazon varies greatly between sites with similar precipitation patterns. Correctly modeling the NEP seasonality with terrestrial ecosystem models has proven difficult. Previous modelling studies have mostly advocated incorporating processes that act to reduce water stress on gross primary productivity (GPP) during the dry season such as including deep soils and roots, plant-mediated hydraulic redistribution of soil moisture, and increased dry season leaf litter generation which reduces leaf age and thus increases photosynthetic capacity. Recent observations, however, indicate that seasonality in heterotrophic respiration also contributes to the observed seasonal cycle of NEP. Here, we use the dynamic vegetation model CLASS-CTEM - without deep soils or roots, hydraulic redistribution of soil moisture or increased dry season litter generation - at two Large-Scale Biosphere-Atmosphere Experiment (LBA) sites (Tapajós km 83 and Jarú Reserve). These LBA sites exhibit opposite seasonal NEP cycles despite similar meteorological conditions. Our simulations are able to reproduce the observed NEP seasonality at both sites. Simulated GPP, heterotrophic respiration, latent and sensible heat fluxes, litter fall rate, soil moisture and temperature, and basic vegetation state are also compared with available observation-based estimates which provide confidence that the model overall behaves realistically at the two sites. Our results indicate that appropriately representing the influence of soil texture and depth, through soil moisture, on seasonal patterns of GPP and, especially, heterotrophic respiration is important to correctly simulating NEP seasonality.

  5. Different responses of soil respiration and its components to nitrogen addition among biomes: a meta-analysis.

    PubMed

    Zhou, Lingyan; Zhou, Xuhui; Zhang, Baocheng; Lu, Meng; Luo, Yiqi; Liu, Lingli; Li, Bo

    2014-07-01

    Anthropogenic activities have increased nitrogen (N) deposition by threefold to fivefold over the last century, which may considerably affect soil respiration (Rs). Although numerous individual studies and a few meta-analyses have been conducted, it remains controversial as to how N addition affects Rs and its components [i.e., autotrophic (Ra) and heterotrophic respiration (Rh)]. To reconcile the difference, we conducted a comprehensive meta-analysis of 295 published studies to examine the responses of Rs and its components to N addition in terrestrial ecosystems. We also assessed variations in their responses in relation to ecosystem types, environmental conditions, and experimental duration (DUR). Our results show that N addition significantly increased Rs by 2.0% across all biomes but decreased by 1.44% in forests and increased by 7.84% and 12.4% in grasslands and croplands, respectively (P < 0.05). The differences may largely result from diverse responses of Ra to N addition among biomes with more stimulation of Ra in croplands and grasslands compared with no significant change in forests. Rh exhibited a similar negative response to N addition among biomes except that in croplands, tropical and boreal forests. Methods of partitioning Rs did not induce significant differences in the responses of Ra or Rh to N addition, except that Ra from root exclusion and component integration methods exhibited the opposite responses in temperate forests. The response ratios (RR) of Rs to N addition were positively correlated with mean annual temperature (MAT), with being more significant when MAT was less than 15 °C, but negatively with DUR. In addition, the responses of Rs and its components to N addition largely resulted from the changes in root and microbial biomass and soil C content as indicated by correlation analysis. The response patterns of Rs to N addition as revealed in this study can be benchmarks for future modeling and experimental studies. PMID:24323545

  6. An Experimental Comparison of Two Methods on Photosynthesis Driving Soil Respiration: Girdling and Defoliation

    PubMed Central

    Jing, Yanli; Guan, Dexin; Wu, Jiabing; Wang, Anzhi; Jin, Changjie; Yuan, Fenghui

    2015-01-01

    Previous studies with different experimental methods have demonstrated that photosynthesis significantly influences soil respiration (RS). To compare the experimental results of different methods, RS after girdling and defoliation was measured in five-year-old seedlings of Fraxinus mandshurica from June to September. Girdling and defoliation significantly reduced RS by 33% and 25% within 4 days, and 40% and 32% within the entire treatment period, respectively. The differential response of RS to girdling and defoliation was a result of the over-compensation for RS after girdling and redistribution of stored carbon after defoliation. No significant effect on RS was observed between girdling and defoliation treatment, while the soluble sugar content in fine roots was higher in defoliation than in girdling treatment, indicating that defoliation had less compensation effect for RS after interrupting photosynthates supply. We confirm the close coupling of RS with photosynthesis and recommend defoliation for further studies to estimate the effect of photosynthesis on RS. PMID:26177498

  7. Soil respiration at mean annual temperature predicts annual total across vegetation types and biomes

    PubMed Central

    Bahn, M.; Reichstein, M.; Davidson, E. A.; Grünzweig, J.; Jung, M.; Carbone, M. S.; Epron, D.; Misson, L.; Nouvellon, Y.; Roupsard, O.; Savage, K.; Trumbore, S. E.; Gimeno, C.; Yuste, J. Curiel; Tang, J.; Vargas, R.; Janssens, I. A.

    2011-01-01

    Soil respiration (SR) constitutes the largest flux of CO2 from terrestrial ecosystems to the atmosphere. However, there still exist considerable uncertainties as to its actual magnitude, as well as its spatial and interannual variability. Based on a reanalysis and synthesis of 80 site-years for 57 forests, plantations, savannas, shrublands and grasslands from boreal to tropical climates we present evidence that total annual SR is closely related to SR at mean annual soil temperature (SRMAT), irrespective of the type of ecosystem and biome. This is theoretically expected for non water-limited ecosystems within most of the globally occurring range of annual temperature variability and sensitivity (Q10). We further show that for seasonally dry sites where annual precipitation (P) is lower than potential evapotranspiration (PET), annual SR can be predicted from wet season SRMAT corrected for a factor related to P/PET. Our finding indicates that it can be sufficient to measure SRMAT for obtaining a well constrained estimate of its annual total. This should substantially increase our capacity for assessing the spatial distribution of soil CO2 emissions across ecosystems, landscapes and regions, and thereby contribute to improving the spatial resolution of a major component of the global carbon cycle. PMID:23293656

  8. Draft Genome Sequence of Anaeromyxobacter sp. Strain PSR-1, an Arsenate-Respiring Bacterium Isolated from Arsenic-Contaminated Soil.

    PubMed

    Tonomura, Mimori; Ehara, Ayaka; Suzuki, Haruo; Amachi, Seigo

    2015-01-01

    Here, we report a draft genome sequence of Anaeromyxobacter sp. strain PSR-1, an arsenate-respiring bacterium isolated from arsenic-contaminated soil. It contained three distinct arsenic resistance gene clusters (ars operons), while no respiratory arsenate reductase gene (arr) was identified. PMID:25977440

  9. Draft Genome Sequence of Anaeromyxobacter sp. Strain PSR-1, an Arsenate-Respiring Bacterium Isolated from Arsenic-Contaminated Soil

    PubMed Central

    Tonomura, Mimori; Ehara, Ayaka; Suzuki, Haruo

    2015-01-01

    Here, we report a draft genome sequence of Anaeromyxobacter sp. strain PSR-1, an arsenate-respiring bacterium isolated from arsenic-contaminated soil. It contained three distinct arsenic resistance gene clusters (ars operons), while no respiratory arsenate reductase gene (arr) was identified. PMID:25977440

  10. Forest soil respiration reflects plant productivity across a temperature gradient in the Alps.

    PubMed

    Caprez, Riccarda; Niklaus, Pascal A; Körner, Christian

    2012-12-01

    Soil respiration (R (s)) plays a key role in any consideration of ecosystem carbon (C) balance. Based on the well-known temperature response of respiration in plant tissue and microbes, R (s) is often assumed to increase in a warmer climate. Yet, we assume that substrate availability (labile C input) is the dominant influence on R (s) rather than temperature. We present an analysis of NPP components and concurrent R (s) in temperate deciduous forests across an elevational gradient in Switzerland corresponding to a 6 K difference in mean annual temperature and a considerable difference in the length of the growing season (174 vs. 262 days). The sum of the short-lived NPP fractions ("canopy leaf litter," "understory litter," and "fine root litter") did not differ across this thermal gradient (+6 % from cold to warm sites, n.s.), irrespective of the fact that estimated annual forest wood production was more than twice as high at low compared to high elevations (largely explained by the length of the growing season). Cumulative annual R (s) did not differ significantly between elevations (836 ± 5 g C m(-2) a(-1) and 933 ± 40 g C m(-2) a(-1) at cold and warm sites, +12 %). Annual soil CO(2) release thus largely reflected the input of labile C and not temperature, despite the fact that R (s) showed the well-known short-term temperature response within each site. However, at any given temperature, R (s) was lower at the warm sites (downregulation). These results caution against assuming strong positive effects of climatic warming on R (s), but support a close substrate relatedness of R (s). PMID:22684867

  11. Remote sensing-based estimation of annual soil respiration at two contrasting forest sites

    NASA Astrophysics Data System (ADS)

    Huang, Ni; Gu, Lianhong; Black, T. Andrew; Wang, Li; Niu, Zheng

    2015-11-01

    Soil respiration (Rs), an important component of the global carbon cycle, can be estimated using remotely sensed data, but the accuracy of this technique has not been thoroughly investigated. In this study, we proposed a methodology for the remote estimation of annual Rs at two contrasting FLUXNET forest sites (a deciduous broadleaf forest and an evergreen needleleaf forest). A version of the Akaike's information criterion was used to select the best model from a range of models for annual Rs estimation based on the remotely sensed data products from the Moderate Resolution Imaging Spectroradiometer and root-zone soil moisture product derived from assimilation of the NASA Advanced Microwave Scanning Radiometer soil moisture products and a two-layer Palmer water balance model. We found that the Arrhenius-type function based on nighttime land surface temperature (LST-night) was the best model by comprehensively considering the model explanatory power and model complexity at the Missouri Ozark and BC-Campbell River 1949 Douglas-fir sites. In addition, a multicollinearity problem among LST-night, root-zone soil moisture, and plant photosynthesis factor was effectively avoided by selecting the LST-night-driven model. Cross validation showed that temporal variation in Rs was captured by the LST-night-driven model with a mean absolute error below 1 µmol CO2 m-2 s-1 at both forest sites. An obvious overestimation that occurred in 2005 and 2007 at the Missouri Ozark site reduced the evaluation accuracy of cross validation because of summer drought. However, no significant difference was found between the Arrhenius-type function driven by LST-night and the function considering LST-night and root-zone soil moisture. This finding indicated that the contribution of soil moisture to Rs was relatively small at our multiyear data set. To predict intersite Rs, maximum leaf area index (LAImax) was used as an upscaling factor to calibrate the site-specific reference respiration

  12. Microbial respiration and kinetics of extracellular enzymes activities through rhizosphere and detritusphere at agricultural site

    NASA Astrophysics Data System (ADS)

    Löppmann, Sebastian; Blagodatskaya, Evgenia; Kuzyakov, Yakov

    2014-05-01

    detritivore communities in the soil. The kinetics (Km and Vmax) of four extracellular hydrolytic enzymes responsible for C- and phosphorous-cycle (β-glucosidase, β-xylosidase, β-cellobiohydrolase and acid phosphatase), microbial biomass, basal respiration (BR) and substrate-induced respiration (SIR) were measured in rhizosphere, detritusphere and control from 0 - 10 and 10 - 20 cm. The metabolic quotient (qCO2) was calculated as specific indicator for efficiency of microbial substrate utilization. We observed clear differences in enzymes activities at low and high concentrations of substrate. At substrate saturation enzyme activity rates of were significantly higher in rooted plots compared to litter amended plots, whereas at lower concentration no treatment effect could be found. The BR, SIR and qCO2 values were significantly higher at 0 - 10 cm of the planted treatment compared to litter and control plots, revealing a significantly higher respiration at lower efficiency of microbial substrate utilization in the rhizosphere. The Michaelis-Menten constant (Km) decreased with depth, especially for β-glucosidase, acid phosphatase and β-xylosidase, indicating higher substrate affinity of microorganisms in deeper soil and therefore different enzyme systems functioning. The substrate affinity factor (Vmax/Km) increased 2-fold with depth for various enzymes, reflecting a switch of predominantly occurring microbial strategies. Vmax/Km ratio indicated relative domination of zymogenous microbial communities (r-strategists) in 0 - 10 cm depth as compared with 10 - 20 cm depth where the K-strategists dominated.

  13. Impact of treated wastewater on growth, respiration and hydraulic conductivity of citrus root systems in light and heavy soils.

    PubMed

    Paudel, Indira; Cohen, Shabtai; Shaviv, Avi; Bar-Tal, Asher; Bernstein, Nirit; Heuer, Bruria; Ephrath, Jhonathan

    2016-06-01

    Roots interact with soil properties and irrigation water quality leading to changes in root growth, structure and function. We studied these interactions in an orchard and in lysimeters with clay and sandy loam soils. Minirhizotron imaging and manual sampling showed that root growth was three times lower in the clay relative to sandy loam soil. Treated wastewater (TWW) led to a large reduction in root growth with clay (45-55%) but not with sandy loam soil (<20%). Treated wastewater increased salt uptake, membrane leakage and proline content, and decreased root viability, carbohydrate content and osmotic potentials in the fine roots, especially in clay. These results provide evidence that TWW challenges and damages the root system. The phenology and physiology of root orders were studied in lysimeters. Soil type influenced diameter, specific root area, tissue density and cortex area similarly in all root orders, while TWW influenced these only in clay soil. Respiration rates were similar in both soils, and root hydraulic conductivity was severely reduced in clay soil. Treated wastewater increased respiration rate and reduced hydraulic conductivity of all root orders in clay but only of the lower root orders in sandy loam soil. Loss of hydraulic conductivity increased with root order in clay and clay irrigated with TWW. Respiration and hydraulic properties of all root orders were significantly affected by sodium-amended TWW in sandy loam soil. These changes in root order morphology, anatomy, physiology and hydraulic properties indicate rapid and major modifications of root systems in response to differences in soil type and water quality. PMID:27022106

  14. Soil carbon and soil respiration in conservation agriculture with vegetables in Siem Reap, Cambodia

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A balance between food production and environmental protection is required to sustainably feed a growing population. The resource saving concept of conservation agriculture aims to achieve this balance through implementing simultaneously three conservation practices; no-till, continuous soil cover, ...

  15. Temporal variations of soil respiration in a moso bamboo (Phyllostachys pubescens) forest in central Taiwan

    NASA Astrophysics Data System (ADS)

    Hsieh, I.-Fang; Kume, Tomonori; Ke, Po-Ju; Wang, Ya-Nan; Hung, Chih-Yuan

    2013-04-01

    Understanding soil respiration (Rs) in moso bamboo stands is crucial for accessing potential impact of bamboo invasion on terrestrial carbon cycle in Asian regions. We aimed to evaluate the seasonal and diurnal variations of Rs in a moso bamboo (Phyllostachys pubescens) forest in central Taiwan with their abiotic drivers. We selected a 200m2 plot in a conservatively managed moso bamboo stand in Natinal Taiwan University Forest, central Taiwan. The 20 measuring locations were set in a 200m2 plot, in which we inserted a 5-cm collar for Rs measurement. Rs rates were measured from April 2012 to February 2013 by using a closed dynamic chamber system. Once a month, we observed the Rs averaged over the 20 locations and the diurnal variation at 2 locations with soil temperature (Ts) and soil volumetric water contents (SWC) measurements. Over 10-month observation, results showed the highest Rs and the most significant spatial variations in Rs in the summer seasons with maximum Rs averaged over 20 locations of 4 - 8.36 μmol m-2s-1. Seasonal variations of the Rs were considerably corresponded to both Ts and SWC with different patterns. The relationship between Rs and Ts can be described with the exponential Q10 function, in which a comparatively higher Q10 value of 6.45 was estimated than that of previous averages (= 2 to 3). The Rs-SWC relationship shows that soil respiration increased with increases in SWC of below 45%, while the Rs declined with increases in SWC of over 45%. Also, Rs occasionally showed distinctive diurnal variations. In the measurement campaign of June, we found the maximum Rs rate exceed twice the minimum rate (from 3.43 to 9.91 μmol m-2s-1). Ts variation is likely to explain the diurnal variation of Rs rather than SWC. Based on the trenching and root exclusion methods, we also quantified partitioning Rs into autotrophic and heterotrophic sources. Further, we discussed characteristics of Rs in the moso bamboo forest by comparing with those of previous

  16. Synthesizing effects of precipitation manipulation on plant production and soil respiration - results and challenges

    NASA Astrophysics Data System (ADS)

    Vicca, Sara; Estiarte, Marc; Bahn, Michael; Peñuelas, Josep; Janssens, Ivan

    2013-04-01

    We compiled a database containing data from over 70 experimental sites where precipitation was manipulated. These experiments cover different biomes (mainly tropical forests, temperate forests and grasslands, temperate and Mediterranean shrublands), but the majority of experiments was performed in the temperate zone. From these experiments, we collected (among others) available data for plant biomass and biomass production, leaf gas exchange, leaf and soil chemistry and soil respiration. Because experiments differed largely in the timing, duration and magnitude of the manipulation, our aim was to first quantify the manipulation and bring all experiments to a common denominator reflecting the (plant) available water. The data needed for such quantification of the manipulation are, however, available for very few experiments. Analyses that go beyond a meta-analytical approach (in which the magnitude of the manipulation is typically neglected) are therefore hampered. In order to avoid problems related to the magnitude of the manipulation, we focussed the analyses of soil respiration (Rsoil) on within-experiment trends. We tested whether a simple temperature-soil moisture-model that fits well to the Rsoil measurements of the control plots can be used to predict the Rsoil measurements for the treatment plots. For several experiments we found that low predictability was not only related to extrapolation beyond the range of SWC in the control plots. Apparently, the manipulation had altered the response of Rsoil to temperature and/or SWC in the treatment plots to a degree which was not predictable from the controls. Besides Rsoil, we also analyzed responses of ANPP to reduced precipitation. A mixed effects modelling approach (which accounts for clustering of observations from sites with multiple years of data and/or multiple manipulations) revealed that ANPP was mainly determined by the site mean annual precipitation (MAP). Additional variation was explained by actual

  17. Temporal changes in soil water repellency linked to the soil respiration and CH4 and CO2 fluxes

    NASA Astrophysics Data System (ADS)

    Qassem, Khalid; Urbanek, Emilia; van Keulen, Geertje

    2014-05-01

    Soil water repellency (SWR) is known to be a spatially and temporally variable phenomenon. The seasonal changes in soil moisture lead to development of soil water repellency, which in consequence may affect the microbial activity and in consequence alter the CO2 and CH4 fluxes from soils. Soil microbial activity is strongly linked to the temperature and moisture status of the soil. In terms of CO2 flux intermediate moisture contents are most favourable for the optimal microbial activity and highest CO2 fluxes. Methanogenesis occurs primarily in anaerobic water-logged habitats while methanotrophy is a strictly aerobic process. In the study we hypothesise that the changes in CO2 and CH4 fluxes are closely linked to critical moisture thresholds for soil water repellency. This research project aims to adopt a multi-disciplinary approach to comprehensively determine the effect of SWR on CO2 and CH4 fluxes. Research is conducted in situ at four sites exhibiting SWR in the southern UK. Flux measurements are carried out concomitant with meteorological and SWR observations Field observations are supported by laboratory measurements carried out on intact soil samples collected at the above identified field sites. The laboratory analyses are conducted under constant temperatures with controlled changes of soil moisture content. Methanogenic and Methanotrophic microbial populations are being analysed at different SWR and moisture contents using the latest metagenomic and metatranscriptomic approaches. Currently available data show that greenhouse gas flux are closely linked with soil moisture thresholds for SWR development.

  18. [Spatial Heterogeneity of Soil Respiration in a Planted Larch Forest in Shanxi Plateau].

    PubMed

    Yan, Jun-xia; Li, Hong-jian; Li, Jun-jian; Wu, Jiang-xing

    2015-05-01

    Based on the data from a planted larch forest in Panquangou Natural Reserve of Shanxi Province, at three sampling scales (4, 2, and 1 m, respectively), soil respiration (Rs) and its affecting factors including soil temperature at 5 cm (T5), 10 cm (T10), and 15 cm (T15) depths, soil water content (Ws), litter mass (Lw), litter moisture (Lm), soil total carbon (C), and soil total nitrogen ( N) were determined. The spatial heterogeneities of Rs and the environmental factors were further analyzed and their intrinsic correlations were established. The results of traditional statistics showed that the spatial variations of Rs and the all measured factors were in the middle range; Rs were highly significantly positively correlated with T10, T15, and N (P < 0.01); significantly positively correlated with Lm (P < 0.05); highly significantly negatively correlated with C/N ratio (P < 0.01); and not significantly correlated with T5, Ws, Lw and C (P > 0.05). Multiple stepwise regression analysis indicated that the four factors of Lm, T10, N, and Ws together accounted for 36% of Rs heterogeneity. The results of geo-statistical analysis demonstrated that Rs was in a medium spatial autocorrelation; random and structural factors accounted for 39.5% and 60.5% of Rs heterogeneity, respectively. And the factors such as climate, landform, and soil played a leading role. The results also illustrated that the ranges for soil factors were different and the range for both Rs and T10 was 25 meters. The fractal dimension of the soil index was in the following order: Lw and C/N ratio (1.95) > N (1.91) > C (1.89) > Rs (1.78) > Lm (1.77 ) > Ws (1.69) > T10 (1.42). The spatial distribution of Rs was in consistent agreement with those of T10, Lm, C, and N; but different with those of Ws and C/N ratio. With a fixed cofidence level and certain estimated accuracy, the required sampling number of each item differed, corresponding to its spatial variation degree. PMID:26314132

  19. Understanding environmental drivers in the regulation of soil respiration dynamics after fire in semi-arid ecosystems

    NASA Astrophysics Data System (ADS)

    Muñoz-Rojas, Miriam; Lewandrowski, Wolfgang; Erickson, Todd E.; Dixon, Kingsley W.; Merritt, David J.

    2016-04-01

    Keywords: Pilbara, soil CO2 efflux, soil C, soil moisture, soil temperature Introduction Soil respiration (Rs) has become a major research focus given the increase in atmospheric CO2 emissions and the large contribution of these CO2 fluxes from soils (Van Groenigen et al., 2014). In addition to its importance in the global C cycle, Rs is a fundamental indicator of soil health and quality that reflects the level of microbial activity and provides an indication of the ability of soils to support plant growth (Oyonarte et al., 2012; Munoz-Rojas et al., 2015). Wildfires can have a significant impact on Rs rates, with the scale of the impact depending on environmental factors such as temperature and moisture, and organic C content in the soil. Vegetation cover can have a significant effect on regulating organic C contents; and while advances are made into understanding the effects of fire on organic C contents and CO2 fluxes (Granged et al., 2011; Willaarts et al., 2015; Muñoz-Rojas et al., 2016), there is limited knowledge of the variability of Rs across ecosystem types, vegetation communities, and responses to fire. In this research we aimed to assess the impacts of a wildfire on the soil CO2 fluxes and soil respiration in a semi-arid ecosystem of Western Australia (Pilbara biogeographical region), and to understand the main environmental drivers controlling these fluxes in different vegetation types. The study has application for other arid and semi-arid regions of the world. Methods The study area was selected following a wildfire that affected 25 ha in February 2014. Twelve plots were established in the burnt site (B) within a 400 m2 area, and 12 plots in an adjacent unburnt control site. At each site, three plots were installed below the canopy of each of the most representative vegetation types of the areas: Eucalyptus trees, Acacia shrubs and Triodia grasses, and three on bare soil. Soil sampling and measurement of soil CO2 efflux, temperature and moisture were

  20. A practical approach for uncertainty quantification of high-frequency soil respiration using Forced Diffusion chambers

    NASA Astrophysics Data System (ADS)

    Lavoie, Martin; Phillips, C. L.; Risk, David

    2015-01-01

    paper examines the sources of uncertainty for the Forced Diffusion (FD) chamber soil respiration (Rs) measurement technique and demonstrates a protocol for uncertainty quantification that could be appropriate with any soil flux technique. Here we sought to quantify and compare the three primary sources of uncertainty in Rs: (1) instrumentation error; (2) scaling error, which stems from the spatial variability of Rs; and (3) random error, which arises from stochastic or unpredictable variation in environmental drivers and was quantified from repeated observations under a narrow temperature, moisture, and time range. In laboratory studies, we found that FD instrumentation error remained constant as Rs increased. In field studies from five North American ecosystems, we found that as Rs increased from winter to peak growing season, random error increased linearly with average flux by about 40% of average Rs. Random error not only scales with soil flux but scales in a consistent way (same slope) across ecosystems. Scaling error, measured at one site, similarly increased linearly with average Rs, by about 50% of average Rs. Our findings are consistent with previous findings for both soil fluxes and eddy covariance fluxes across other northern temperate ecosystems that showed random error scales linearly with flux magnitude with a slope of ~0.2. Although the mechanistic basis for this scaling of random error is unknown, it is suggestive of a broadly applicable rule for predicting flux random error. Also consistent with previous studies, we found the random error of FD follows a Laplace (double-exponential) rather than a normal (Gaussian) distribution.

  1. Multi-Year Lags between Forest Browning and Soil Respiration at High Northern Latitudes

    PubMed Central

    Bond-Lamberty, Ben; Bunn, Andrew G.; Thomson, Allison M.

    2012-01-01

    High-latitude northern ecosystems are experiencing rapid climate changes, and represent a large potential climate feedback because of their high soil carbon densities and shifting disturbance regimes. A significant carbon flow from these ecosystems is soil respiration (RS, the flow of carbon dioxide, generated by plant roots and soil fauna, from the soil surface to atmosphere), and any change in the high-latitude carbon cycle might thus be reflected in RS observed in the field. This study used two variants of a machine-learning algorithm and least squares regression to examine how remotely-sensed canopy greenness (NDVI), climate, and other variables are coupled to annual RS based on 105 observations from 64 circumpolar sites in a global database. The addition of NDVI roughly doubled model performance, with the best-performing models explaining ∼62% of observed RS variability. We show that early-summer NDVI from previous years is generally the best single predictor of RS, and is better than current-year temperature or moisture. This implies significant temporal lags between these variables, with multi-year carbon pools exerting large-scale effects. Areas of decreasing RS are spatially correlated with browning boreal forests and warmer temperatures, particularly in western North America. We suggest that total circumpolar RS may have slowed by ∼5% over the last decade, depressed by forest stress and mortality, which in turn decrease RS. Arctic tundra may exhibit a significantly different response, but few data are available with which to test this. Combining large-scale remote observations and small-scale field measurements, as done here, has the potential to allow inferences about the temporal and spatial complexity of the large-scale response of northern ecosystems to changing climate. PMID:23189202

  2. Temperature-driven seasonal and diel variation in soil respiration in a moist subtropical forest in Puerto Rico

    NASA Astrophysics Data System (ADS)

    Gutiérrez del Arroyo, O.; Wood, T. E.; Lugo, A. E.

    2013-12-01

    Tropical forest soils are the largest natural source of carbon dioxide (CO2) to the atmosphere and have the highest soil respiration rates, globally. Currently, we have little understanding of how this large carbon (C) flux will respond to ongoing changes in climate. Identifying climatic controls and natural variability of soil respiration (Rs) in these ecosystems could improve our ability to predict feedbacks to future climate change. We measured hourly Rs in a secondary, moist subtropical forest in Puerto Rico for a 2-year period using an automated soil respiration system (LI-COR 8100) to determine at what time-scale Rs varies and whether this variability can be explained by abiotic factors such as temperature and moisture. Soil respiration varied significantly at both seasonal and diel time-scales. Mean monthly Rs ranged from 4 to 12 μmol CO2 m-2 s-1 and the seasonal variation was positively correlated with air temperature (p<0.0001, R2=0.69). In addition, Rs was notably reduced immediately following large precipitation events, possibly due to reduced diffusion rates out of the soil or low oxygen availability; however, precipitation was not related to Rs on a seasonal time-scale. Soil respiration also demonstrated significant diel variation, changing from 1.5 to 3.5 μmol CO2 m-2 s-1 throughout the day. As with seasonal variation, Rs was positively correlated to soil temperature (p<0.0001, R2=0.61) on a diel time-scale. Diel Rs was decoupled with soil temperature at midday possibly responding to a depression in photosynthesis, which may pause the transport of photosynthate to the roots. The shape of the temperature-Rs hysteresis effect changed seasonally in concert with air temperature. The significant positive effect of temperature on Rs in this forest, despite low intra-annual variability (<4°C), suggests that soil C loss from moist subtropical forests could increase as global temperatures rise. Diel hysteresis effects of Rs suggest that temperature has both

  3. Plant ecophysiological status and soil micro-organisms control the time-lagged response of ecosystem respiration to environmental changes

    NASA Astrophysics Data System (ADS)

    Salmon, Y.; Barnard, R. L.; Buchmann, N. C.

    2009-12-01

    Carbon stable isotopes are widely used tools to study the carbon exchange between atmosphere and biosphere. In particular, stable carbon isotopes have been used as a natural tracer in many ecosystem studies to investigate environmental controls on carbon turnover time. This tracer-function results from the changes of carbon isotopic signature (δ13C) of newly assimilated sugars due to plant physiological response to environmental changes through photosynthetic discrimination. Previous studies have shown response of δ13C in ecosystem respiration (δ13CR) to environmental changes with a time lag of 1 to 10 days. The observed time lag was attributed to the transfer of newly assimilated carbon from leaves to heterotrophic ecosystem components where it is respired. However, these time-lagged responses were not consistently observed. Therefore, a clear understanding of the mechanisms underlying time-lagged responses to environmental changes of δ13CR is still lacking. This study aims to improve our understanding of plant physiology and soil micro-organisms controls over these time-lagged responses. The first part of this study focus on the impact of plant physiology on the time-lagged response of δ13CR to environmental changes. Wheat plants with different physiological status were obtained by growing them under different nutrient and water availabilities. The response of δ13C of leaf and soil-respired CO2 to a 13C labeling was measured. We found that the measured carbon turnover time was related to plant physiology, in particular leaf conductance (gs). The second part aims to determine if time-lagged responses of δ13CR to environmental changes are only triggered by the transfer of newly assimilated sugars with different δ13C or if these responses are also due to activity of soil micro-organisms. We exposed a set of beech saplings suffering from drought at different temperature to a water pulse mimicking a rain event. To test the importance of carbon transfer from

  4. Salinity effect of irrigation with treated wastewater in basal soil respiration in SE of Spain

    NASA Astrophysics Data System (ADS)

    Morugan, A.; Garcia-Orenes, F.; Mataix-Solera, J.

    2012-04-01

    The use of treated wastewater for the irrigation of agricultural soils is an alternative to utilizing better-quality water, especially in semiarid regions where water shortage is a very serious problem. Wastewater use in agriculture is not a new practice, all over the world this reuse has been common practice for a long time, but the concept is of greater importance currently because of the global water crisis. Replacement of freshwater by treated wastewater is seen as an important conservation strategy contributing to agricultural production, substantial benefits can derive from using this nutrient-rich waste water but there can also be a negative impact. For this reason it is necessary to know precisely the composition of water before applying it to the soil in order to guarantee minimal impact in terms of contamination and salinization. In this work we have been studying, for more than three years, different parameters in calcareous soils irrigated with treated wastewater in an agricultural Mediterranean area located at Biar (Alicante, SE Spain), with a crop of grape (Vitis labrusca). Three types of waters were used for the irrigation of the soil: fresh water (control) (TC), and treated wastewater from secondary (T2) and tertiary treatment (T3). Three different doses of irrigation have been applied to fit the efficiency of the irrigation to the crop and soil type during the study period. A soil sampling was carried out every four months. We show the results of the evolution of basal soil respiration (BSR), and its relationship with other parameters. We observed a similar pattern of behavior for BSR between treatments, a decrease at the first eighteen months of irrigation and an increase at the end of study. In our study case, the variations of BSR obtained for all the treatments seem to be closely related to the dose and frequency of irrigation and the related soil wetting and drying cycles. However, the results showed a negative correlation between BSR and

  5. Mitochondrial impairment by PPAR agonists and statins identified via immunocaptured OXPHOS complex activities and respiration.

    PubMed

    Nadanaciva, Sashi; Dykens, James A; Bernal, Autumn; Capaldi, Roderick A; Will, Yvonne

    2007-09-15

    Mitochondrial impairment is increasingly implicated in the etiology of toxicity caused by some thiazolidinediones, fibrates, and statins. We examined the effects of members of these drug classes on respiration of isolated rat liver mitochondria using a phosphorescent oxygen sensitive probe and on the activity of individual oxidative phosphorylation (OXPHOS) complexes using a recently developed immunocapture technique. Of the six thiazolidinediones examined, ciglitazone, troglitazone, and darglitazone potently disrupted mitochondrial respiration. In accord with these data, ciglitazone and troglitazone were also potent inhibitors of Complexes II+III, IV, and V, while darglitazone predominantly inhibited Complex IV. Of the six statins evaluated, lovastatin, simvastatin, and cerivastatin impaired mitochondrial respiration the most, with simvastatin and lovastatin impairing multiple OXPHOS Complexes. Within the class of fibrates, gemfibrozil more potently impaired respiration than fenofibrate, clofibrate, or ciprofibrate. Gemfibrozil only modestly inhibited Complex I, fenofibrate inhibited Complexes I, II+III, and V, and clofibrate inhibited Complex V. Our findings with the two complementary methods indicate that (1) some members of each class impair mitochondrial respiration, whereas others have little or no effect, and (2) the rank order of mitochondrial impairment accords with clinical adverse events observed with these drugs. Since the statins are frequently co-prescribed with the fibrates or thiazolidinediones, various combinations of these three drug classes were also analyzed for their mitochondrial effects. In several cases, the combination additively uncoupled or inhibited respiration, suggesting that some combinations are more likely to yield clinically relevant drug-induced mitochondrial side effects than others. PMID:17658574

  6. Mitochondrial impairment by PPAR agonists and statins identified via immunocaptured OXPHOS complex activities and respiration

    SciTech Connect

    Nadanaciva, Sashi; Dykens, James A.; Bernal, Autumn; Capaldi, Roderick A.; Will, Yvonne

    2007-09-15

    Mitochondrial impairment is increasingly implicated in the etiology of toxicity caused by some thiazolidinediones, fibrates, and statins. We examined the effects of members of these drug classes on respiration of isolated rat liver mitochondria using a phosphorescent oxygen sensitive probe and on the activity of individual oxidative phosphorylation (OXPHOS) complexes using a recently developed immunocapture technique. Of the six thiazolidinediones examined, ciglitazone, troglitazone, and darglitazone potently disrupted mitochondrial respiration. In accord with these data, ciglitazone and troglitazone were also potent inhibitors of Complexes II + III, IV, and V, while darglitazone predominantly inhibited Complex IV. Of the six statins evaluated, lovastatin, simvastatin, and cerivastatin impaired mitochondrial respiration the most, with simvastatin and lovastatin impairing multiple OXPHOS Complexes. Within the class of fibrates, gemfibrozil more potently impaired respiration than fenofibrate, clofibrate, or ciprofibrate. Gemfibrozil only modestly inhibited Complex I, fenofibrate inhibited Complexes I, II + III, and V, and clofibrate inhibited Complex V. Our findings with the two complementary methods indicate that (1) some members of each class impair mitochondrial respiration, whereas others have little or no effect, and (2) the rank order of mitochondrial impairment accords with clinical adverse events observed with these drugs. Since the statins are frequently co-prescribed with the fibrates or thiazolidinediones, various combinations of these three drug classes were also analyzed for their mitochondrial effects. In several cases, the combination additively uncoupled or inhibited respiration, suggesting that some combinations are more likely to yield clinically relevant drug-induced mitochondrial side effects than others.

  7. [Effects of conversion of natural broad-leaved forest to Chinese fir plantation on soil respiration in subtropical China].

    PubMed

    Zhang, Rui; Bai, Yang; Liu, Juan; Jiang, Pei-kun; Zhou, Guo-mo; Wu, Jia-sen; Tong, Zhi-peng; Li, Yong-fu

    2015-10-01

    Soil CO2 effluxes in natural broad-leaved forest and the conversed Chinese fir plantation in Linglong Mountains Scenic of Zhejiang Province were evaluated by using static closed chamber and gas chromatography method. The results showed that soil CO2 efflux showed consistent seasonal dynamics in natural broad-leaved forest and Chinese fir plantation, with the maximums observed in summer and autumn, the minimums in winter and spring. Soil CO2 effluxes were 20.0-111.3 and 4.1-118.6 mg C . m-2 . h-1 in natural broad-leaved forest and Chinese fir plantation, respectively. The cumulative soil CO2 emission of natural broad-leaved forest (16.46 t CO2 . hm-2 . a-1) was significantly higher than that of Chinese fir plantation (11.99 t CO2 . hm-2 . a-1). Soil moisture did not affect soil CO2 efflux. There was a significant relationship between soil CO2 efflux and soil temperature at 5 cm depth. There was no significant relationship between soil CO2 efflux of natural broad-leaved forest and water soluble organic carbon content, while water soluble organic carbon content affected significantly soil CO2 efflux in Chinese fir plantation. Converting the natural broad-leaved forest to Chinese fir plantation reduced soil CO2 efflux significantly but improved the sensitivity of soil respiration to environmental factors. PMID:26995901

  8. Charcoal produced by prescribed fire increases dissolved organic carbon and soil microbial activity

    NASA Astrophysics Data System (ADS)

    Poon, Cheryl; Jenkins, Meaghan; Bell, Tina; Adams, Mark

    2014-05-01

    In Australian forests fire is an important driver of carbon (C) storage. When biomass C is combusted it is transformed into vegetation residue (charcoal) and deposited in varying amounts and forms onto soil surfaces. The C content of charcoal is high but is largely in a chemically stable form of C, which is highly resistance to microbial decomposition. We conducted two laboratory incubations to examine the influence of charcoal on soil microbial activity as indicated by microbial respiration. Seven sites were chosen in mixed species eucalypt forest in Victoria, Australia. Soil was sampled prior to burning to minimise the effects of heating or addition of charcoal during the prescribed burn. Charcoal samples were collected from each site after the burn, homogenised and divided into two size fractions. Prior to incubation, soils were amended with the two size fractions (<1 and 1-4.75 mm) and at two rates of amount (2.5 and 5% by soil dry weight). Charcoal-amended soils were incubated in the laboratory for 86 d, microbial respiration was measured nine times at day 1, 3, 8, 15, 23, 30, 45, 59 and 86 d. We found that addition of charcoal resulted in faster rates of microbial respiration compared to unamended soil. Fastest rates of microbial respiration in all four treatments were measured 1 d after addition of charcoal (up to 12 times greater than unamended soil). From 3 to 8 d, respiration rates in all four treatments decreased and only treatments with greater charcoal addition (5%) remained significantly faster than unamended soil. From 15 d to 86 d, all treatments had respiration rates similar to unamended soil. Overall, adding greater amount of charcoal (5%) resulted in a larger cumulative amount of CO2 released over the incubation period when compared to unamended soil. The second laboratory incubation focused on the initial changes in soil nutrient and microbial respiration after addition of charcoal over a 72 h period. Charcoal (<2 mm) was added at rate of 5% to

  9. Impaired ALDH2 activity decreases the mitochondrial respiration in H9C2 cardiomyocytes.

    PubMed

    Mali, Vishal R; Deshpande, Mandar; Pan, Guodong; Thandavarayan, Rajarajan A; Palaniyandi, Suresh S

    2016-02-01

    Reactive oxygen species (ROS)-mediated reactive aldehydes induce cellular stress. In cardiovascular diseases such as ischemia-reperfusion injury, lipid-peroxidation derived reactive aldehydes such as 4-hydroxy-2-nonenal (4HNE) are known to contribute to the pathogenesis. 4HNE is involved in ROS formation, abnormal calcium handling and more importantly defective mitochondrial respiration. Aldehyde dehydrogenase (ALDH) superfamily contains NAD(P)(+)-dependent isozymes which can detoxify endogenous and exogenous aldehydes into non-toxic carboxylic acids. Therefore we hypothesize that 4HNE afflicts mitochondrial respiration and leads to cell death by impairing ALDH2 activity in cultured H9C2 cardiomyocyte cell lines. H9C2 cardiomyocytes were treated with 25, 50 and 75 μM 4HNE and its vehicle, ethanol as well as 25, 50 and 75 μM disulfiram (DSF), an inhibitor of ALDH2 and its vehicle (DMSO) for 4 h. 4HNE significantly decreased ALDH2 activity, ALDH2 protein levels, mitochondrial respiration and mitochondrial respiratory reserve capacity, and increased 4HNE adduct formation and cell death in cultured H9C2 cardiomyocytes. ALDH2 inhibition by DSF and ALDH2 siRNA attenuated ALDH2 activity besides reducing ALDH2 levels, mitochondrial respiration and mitochondrial respiratory reserve capacity and increased cell death. Our results indicate that ALDH2 impairment can lead to poor mitochondrial respiration and increased cell death in cultured H9C2 cardiomyocytes. PMID:26577527

  10. Soil microbial respiration rate and temperature sensitivity along a north-south forest transect in eastern China: Patterns and influencing factors

    NASA Astrophysics Data System (ADS)

    Wang, Qing; He, Nianpeng; Yu, Guirui; Gao, Yang; Wen, Xuefa; Wang, Rongfu; Koerner, Sally E.; Yu, Qiang

    2016-02-01

    Soil organic matter is one of the most important carbon (C) pools in terrestrial ecosystems, and future warming from climate change will likely alter soil C storage via temperature effects on microbial respiration. In this study, we collected forest soils from eight locations along a 3700 km north-south transect in eastern China (NSTEC). For 8 weeks these soils were incubated under a periodically changing temperature range of 6-30°C while frequently measuring soil microbial respiration rate (Rs; each sample about every 20 min). This experimental design allowed us to investigate Rs and the temperature sensitivity of Rs (Q10) along the NSTEC. Both Rs at 20°C (R20) and Q10 significantly increased (logarithmically) with increasing latitude along the NSTEC suggesting that the sensitivity of soil microbial respiration to changing temperatures is higher in forest soils from locations with lower temperature. Our findings from an incubation experiment provide support for the hypothesis that temperature sensitivity of soil microbial respiration increases with biochemical recalcitrance (C quality-temperature hypothesis) across forest soils on a large spatial scale. Furthermore, microbial properties primarily controlled the observed patterns of R20, whereas both substrate and microbial properties collectively controlled the observed patterns of Q10. These findings advance our understanding of the driving factors (microbial versus substrate properties) of R20 and Q10 as well as the general relationships between temperature sensitivity of soil microbial respiration and environmental factors.

  11. Using 13C-labeled benzene and Raman gas spectroscopy to investigate respiration and biodegradation kinetics following soil contamination

    NASA Astrophysics Data System (ADS)

    Jochum, Tobias; Popp, Juergen; Frosch, Torsten

    2016-04-01

    Soil and groundwater contamination with benzene can cause serious environmental damages. However, many soil microorganisms are capable to adapt and known to strongly control the fate of organic contamination. Cavity enhanced Raman gas spectroscopy (CERS) was applied to investigate the short-term response of indigenous soil bacteria to a sudden surface contamination with benzene regarding the temporal variations of gas products and their exchange rates with the adjacent atmosphere. 13C-labeled benzene was spiked on a silty-loamy soil column (sampled from Hainich National Park, Germany) in order to track and separate the changes in heterotrophic soil respiration - involving 12CO2 and O2 - from the microbial process of benzene degradation, which ultimately forms 13CO2.1 The respiratory quotient (RQ) of 0.98 decreased significantly after the spiking and increased again within 33 hours to a value of 0.72. This coincided with maximum 13CO2 concentration rates (0.63 μ mol m-2 s-1), indicating highest benzene degradation at 33 hours after the spiking event. The diffusion of benzene in the headspace and the biodegradation into 13CO2 were simultaneously monitored and 12 days after the benzene spiking no measurable degradation was detected anymore.1 The RQ finally returned to a value of 0.96 demonstrating the reestablished aerobic respiration. In summary, this study shows the potential of combining Raman gas spectroscopy and stable isotopes to follow soil microbial biodegradation dynamics while simultaneously monitoring the underlying respiration behavior. Support by the Collaborative Research Center 1076 Aqua Diva is kindly acknowledged. We thank Beate Michalzik for soil analysis and discussion. 1. T. Jochum, B. Michalzik, A. Bachmann, J. Popp and T. Frosch, Analyst, 2015, 140, 3143-3149.

  12. Linking microbial exo-enzyme production to biomass stoichiometry, resource availability and soil respiration

    NASA Astrophysics Data System (ADS)

    Ballantyne, F.; Billings, S. A.

    2013-12-01

    The rate of decomposition of soil organic matter (SOM) is influenced by substrate composition, the diffusion of substrates and exoenzymes secreted by microbes to reaction sites, and the reaction kinetics of those exoenzymes. Predicting carbon (C) flow from SOM into respired CO2 thus requires an understanding of microbial resource allocation and physiological responses to their environment, as these are the factors governing exoenzyme synthesis. Without such an understanding, it is difficult to project how warming and changing edaphic characteristics will influence respiratory CO2 losses from soils. In essence, we need to know how changing environmental conditions directly influence microbial resource demands and reaction kinetics in the soil matrix, and how microbes alter their behavior to maintain metabolic function and balanced acquisition of resources. Here, we present elements of a general theoretical framework describing the consequences of interactions between exoenzymes, SOM substrates, microbial resource allocation and microbial stoichiometry. Our model incorporates the kinetics of exoenzyme-substrate interactions, the costs and benefits associated with producing different exoenzymes, regulation of biomass C:N, and substrate availability in the soil matrix. First, we articulate how resource limitation can become manifest during resource allocation to exoenzymes and acquisition from decaying SOM, and the feedbacks between these two types of limitation. Second, we explore how shifting resource availability forces microbes to alter their strategies for synthesizing exoenzymes to promote acquisition of C and N that satisfies demand. In particular, we study how increases and decreases in total SOM substrate availability influence biomass stoichiometry, how changes in relative exoenzyme-substrate reaction kinetics predicted with warming constrain strategies for regulating relative rates of C and N acquisition, and how strategies for stoichiometric regulation

  13. Draft Genome Sequence of Geobacter sp. Strain OR-1, an Arsenate-Respiring Bacterium Isolated from Japanese Paddy Soil

    PubMed Central

    Ehara, Ayaka; Suzuki, Haruo

    2015-01-01

    Here, we report a draft genome sequence of Geobacter sp. strain OR-1, an arsenate-respiring bacterium isolated from Japanese paddy soil. It contained two distinct arsenic islands, one including genes for a respiratory arsenate reductase (Arr) as well as for arsenic resistance (arsD-arsA-acr3-arsR-arrA-arrB) and the second containing only genes for arsenic resistance. PMID:25635012

  14. Draft Genome Sequence of Geobacter sp. Strain OR-1, an Arsenate-Respiring Bacterium Isolated from Japanese Paddy Soil.

    PubMed

    Ehara, Ayaka; Suzuki, Haruo; Amachi, Seigo

    2015-01-01

    Here, we report a draft genome sequence of Geobacter sp. strain OR-1, an arsenate-respiring bacterium isolated from Japanese paddy soil. It contained two distinct arsenic islands, one including genes for a respiratory arsenate reductase (Arr) as well as for arsenic resistance (arsD-arsA-acr3-arsR-arrA-arrB) and the second containing only genes for arsenic resistance. PMID:25635012

  15. Distinct patterns in the diurnal and seasonal variability in four components of soil respiration in a temperate forest under free-air CO2 enrichment

    NASA Astrophysics Data System (ADS)

    Taneva, L.; Gonzalez-Meler, M. A.

    2011-03-01

    Soil respiration (RS) is a major flux in the global carbon (C) cycle and its responses to changing environmental conditions may exert a strong control on the residence time of C in terrestrial ecosystems and in turn influence the atmospheric concentration of greenhouse gases. Soil respiration consists of several components returning C of different nature and age to the atmosphere, with root/rhizosphere respiration often assumed to be the dominant and variable one. Rates of RS vary greatly in time and space and the mechanisms underlying this temporal variability, or the RS components responsible for it, are poorly understood. It is often assumed the Rs and its components are under abiotic control at almost all time scales. In this study, we used the ecosystem 13C tracer at the Duke Forest Free Air CO2 Enrichment site to separate forest RS into four components: root/rhizosphere respiration (RR), litter decomposition (RL), and decomposition of soil organic matter (SOM) of two age classes - up to 8 years old and SOM older than 8 years. We then examined and found that diurnal and seasonal variability in the components of Rs occurred at different magnitudes and directions than total RS. Soil respiration was generally dominated by RSOM during the growing season (44% of daytime RS), especially at night. The contribution of heterotrophic respiration (RSOM and RL) to RS was not constant during the growing season, indicating that the seasonal variability seen in RR alone cannot explain the seasonal variability in RS. Although there was no diurnal variability in RS, there were significant compensatory differences in the contribution of individual RS components to daytime and nighttime rates. The average contribution of RSOM to RS was greater at night (54%) than during the day (44%) whereas the average contribution of RR to total RS was ~30% during the day and ~34% during the night. In contrast, RL constituted 26% of RS during the day and only 12% at night. Interestingly, the

  16. Microbial community composition explains soil respiration responses to changing carbon inputs along an Andes-to-Amazon elevation gradient

    PubMed Central

    Whitaker, Jeanette; Ostle, Nicholas; Nottingham, Andrew T; Ccahuana, Adan; Salinas, Norma; Bardgett, Richard D; Meir, Patrick; McNamara, Niall P; Austin, Amy

    2014-01-01

    1. The Andes are predicted to warm by 3–5 °C this century with the potential to alter the processes regulating carbon (C) cycling in these tropical forest soils. This rapid warming is expected to stimulate soil microbial respiration and change plant species distributions, thereby affecting the quantity and quality of C inputs to the soil and influencing the quantity of soil-derived CO2 released to the atmosphere. 2. We studied tropical lowland, premontane and montane forest soils taken from along a 3200-m elevation gradient located in south-east Andean Peru. We determined how soil microbial communities and abiotic soil properties differed with elevation. We then examined how these differences in microbial composition and soil abiotic properties affected soil C-cycling processes, by amending soils with C substrates varying in complexity and measuring soil heterotrophic respiration (RH). 3. Our results show that there were consistent patterns of change in soil biotic and abiotic properties with elevation. Microbial biomass and the abundance of fungi relative to bacteria increased significantly with elevation, and these differences in microbial community composition were strongly correlated with greater soil C content and C:N (nitrogen) ratios. We also found that RH increased with added C substrate quality and quantity and was positively related to microbial biomass and fungal abundance. 4. Statistical modelling revealed that RH responses to changing C inputs were best predicted by soil pH and microbial community composition, with the abundance of fungi relative to bacteria, and abundance of gram-positive relative to gram-negative bacteria explaining much of the model variance. 5. Synthesis. Our results show that the relative abundance of microbial functional groups is an important determinant of RH responses to changing C inputs along an extensive tropical elevation gradient in Andean Peru. Although we do not make an experimental test of the effects of climate

  17. Response of soil respiration and ecosystem carbon budget to vegetation removal in Eucalyptus plantations with contrasting ages

    PubMed Central

    Wu, Jianping; Liu, Zhanfeng; Huang, Guomin; Chen, Dima; Zhang, Weixin; Shao, Yuanhu; Wan, Songze; Fu, Shenglei

    2014-01-01

    Reforested plantations have substantial effects on terrestrial carbon cycling due to their large coverage area. Although understory plants are important components of reforested plantations, their effects on ecosystem carbon dynamics remain unclear. This study was designed to investigate the effects of vegetation removal/understory removal and tree girdling on soil respiration and ecosystem carbon dynamics in Eucalyptus plantations of South China with contrasting ages (2 and 24 years old). We conducted a field manipulation experiment from 2008 to 2009. Understory removal reduced soil respiration in both plantations, whereas tree girdling decreased soil respiration only in the 2-year-old plantations. The net ecosystem production was approximately three times greater in the 2-year-old plantations (13.4 t C ha−1 yr−1) than in the 24-year-old plantations (4.2 t C h−1 yr−1). The biomass increase of understory plants was 12.6 t ha−1 yr−1 in the 2-year-old plantations and 2.9 t ha−1 yr−1 in the 24-year-old plantations, accounting for 33.9% and 14.1% of the net primary production, respectively. Our findings confirm the ecological importance of understory plants in subtropical plantations based on the 2 years of data. These results also indicate that Eucalyptus plantations in China may be an important carbon sink due to the large plantation area. PMID:25179343

  18. SOIL RESPIRED D13C SIGNATURES REFLECT ROOT EXUDATE OR ROOT TURNOVER SIGNATURES IN AN ELEVATED CO2 AND OZONE MESOCOSM EXPERIMENT

    EPA Science Inventory

    Bulk tissue and root and soil respired d13C signatures were measured throughout the soil profile in a Ponderosa Pine mesocosm experiment exposed to ambient and elevated CO2 concentrations. For the ambient treatment, root (0-1mm, 1-2mm, and >2mm) and soil d13C signatures were ?24...

  19. The age of root and soil respired CO2 in a Pacific Northwest old-growth forest: Implications of seasonality and drought effects on carbon source use

    NASA Astrophysics Data System (ADS)

    Taylor, A.; Hopkins, F. M.; Lai, C.; Xu, X.; Randerson, J. T.; Bush, S.; Ehleringer, J. R.

    2013-12-01

    Abstract Climate change has the potential to impact the carbon (C) cycle in unknown ways. Factors such as temperature, light, and moisture can strongly influence whether forest ecosystems are net sources or sinks of CO2. In this study, we used radiocarbon (14C) to determine the age and source of soil- and root-respired CO2 using a combination of soil chamber and biomass incubation measurements in an old-growth forest at the Wind River Field Station, WA. We had two main goals for this study. The first was to determine if the contribution of recent photosynthate to root respiration changed between spring and summer seasons. 14C measurements were used to determine the average age of respired CO2, since respired CO2 fueled by recent photosynthates have Δ14C values similar to that of the current atmosphere (~ 25‰ in 2012), whereas C stored by trees from prior years would be 30‰ or higher. This study occurred over two growing seasons, examining the effects of seasonality and water stress on root/soil respiration. Because of the summer drought conditions consistently experienced by this old-growth forest, this study provides a new dataset to test the hypothesis that plants allocate their C resources in response to stress. Initial results showed soil organic matter components had Δ14C values 80-120‰ greater than that of the background atmosphere, suggesting turnover times on the order of years to decades. In contrast, root respiration was much lower in Δ14C (~40‰), but still elevated with respect to current atmospheric Δ14C values, suggesting that root respiration was at least partially composed of C stored for > 1 year. The second goal was to partition the contribution of autotrophic to heterotrophic respiration and to determine how this ratio differs on diurnal and seasonal timescales. We used the difference between autotrophic and heterotrophic Δ14C values to partition total soil respiration. Preliminary results for April 2013 showed that ~1/3 of soil

  20. Interactive effects of global change factors on soil respiration and its components: a meta-analysis.

    PubMed

    Zhou, Lingyan; Zhou, Xuhui; Shao, Junjiong; Nie, Yuanyuan; He, Yanghui; Jiang, Liling; Wu, Zhuoting; Hosseini Bai, Shahla

    2016-09-01

    As the second largest carbon (C) flux between the atmosphere and terrestrial ecosystems, soil respiration (Rs) plays vital roles in regulating atmospheric CO2 concentration ([CO2 ]) and climatic dynamics in the earth system. Although numerous manipulative studies and a few meta-analyses have been conducted to determine the responses of Rs and its two components [i.e., autotrophic (Ra) and heterotrophic (Rh) respiration] to single global change factors, the interactive effects of the multiple factors are still unclear. In this study, we performed a meta-analysis of 150 multiple-factor (≥2) studies to examine the main and interactive effects of global change factors on Rs and its two components. Our results showed that elevated [CO2 ] (E), nitrogen addition (N), irrigation (I), and warming (W) induced significant increases in Rs by 28.6%, 8.8%, 9.7%, and 7.1%, respectively. The combined effects of the multiple factors, EN, EW, DE, IE, IN, IW, IEW, and DEW, were also significantly positive on Rs to a greater extent than those of the single-factor ones. For all the individual studies, the additive interactions were predominant on Rs (90.6%) and its components (≈70.0%) relative to synergistic and antagonistic ones. However, the different combinations of global change factors (e.g., EN, NW, EW, IW) indicated that the three types of interactions were all important, with two combinations for synergistic effects, two for antagonistic, and five for additive when at least eight independent experiments were considered. In addition, the interactions of elevated [CO2 ] and warming had opposite effects on Ra and Rh, suggesting that different processes may influence their responses to the multifactor interactions. Our study highlights the crucial importance of the interactive effects among the multiple factors on Rs and its components, which could inform regional and global models to assess the climate-biosphere feedbacks and improve predictions of the future states of the

  1. [Soil respiration characteristics in the clear-cutting site of Quercus aliena var. acuteserrata forest in Xiaolong Mountain in Qinling Mountains].

    PubMed

    Kang, Yong-Xiang; Xia, Guo-Wei; Liu, Jian-Jun; Zhou, Wei; Chen, Guang-Ping

    2014-02-01

    By using Li-6400 portable photosynthesis system with soil chamber, the soil respiration rates (Rs) of Quercus aliena var. acuteserrata forest land (control site) and their clear-cutting site were measured in Xiaolong Mountain of Qinling Mountains from May 2011 to April 2012 to understand the diurnal and monthly dynamics of soil respiration rate and the influences from soil temperature, soil moisture, soil physical and chemical properties. The results showed that both diurnal and monthly dynamics of soil respiration rate presented a single-peak curve, similar to the variation of soil temperature at the clear-cutting and control sites. During the study period, the maximum monthly mean values of soil respiration rate at the clear-cutting and control sites occurred in July (4.63 and 4.01 micromol x m(-2) x s(-1), respectively) and the minimum values presented in February (0.10 and 0.30 micromol x m(-2) x s(-1), respectively). Soil respiration rate in 4-6 months after clear-cutting was higher than at the control site, and became lower afterwards. 89. 6% -90. 8% of soil respiration rate variation was interpreted by the multiple regression models of soil temperature, soil moisture and their interaction at the clear-cutting site, and 94.7%-95.5% at the control site. The Q10 values computed by exponential equations were 3.47-4.22 and 3.54-3.96 at the clear-cutting and control sites, respectively. The C fluxes at the clear-cutting and control sites were 344.8 and 512.9 g x m(-2) annually, and 24.2 and 40.9 g x m(-2) in winter, respectively. PMID:24830231

  2. Coupled soil respiration and transpiration dynamics from tree-scale to catchment scale in dry Rocky Mountain pine forests and the role of snowpack

    NASA Astrophysics Data System (ADS)

    Berryman, E.; Barnard, H. R.; Brooks, P. D.; Adams, H.; Burns, M. A.; Wilson, W.; Stielstra, C. M.

    2013-12-01

    A current ecohydrological challenge is quantifying the exact nature of carbon (C) and water couplings across landscapes. An emerging framework of understanding places plant physiological processes as a central control over soil respiration, the largest source of CO2 to the atmosphere. In dry montane forests, spatial and temporal variability in forest physiological processes are governed by hydrological patterns. Critical feedbacks involving respiration, moisture supply and tree physiology are poorly understood and must be quantified at the landscape level to better predict carbon cycle implications of regional drought under future climate change. We present data from an experiment designed to capture landscape variability in key coupled hydrological and C processes in forests of Colorado's Front Range. Sites encompass three catchments within the Boulder Creek watershed, range from 1480 m to 3021 m above sea level and are co-located with the DOE Niwot Ridge Ameriflux site and the Boulder Creek Critical Zone Observatory. Key hydrological measurements (soil moisture, transpiration) are coupled with soil respiration measurements within each catchment at different landscape positions. This three-dimensional study design also allows for the examination of the role of water subsidies from uplands to lowlands in controlling respiration. Initial findings from 2012 reveal a moisture threshold response of the sensitivity of soil respiration to temperature. This threshold may derive from tree physiological responses to variation in moisture availability, which in turn is controlled by the persistence of snowpack. Using data collected in 2013, first, we determine whether respiration moisture thresholds represent triggers for transpiration at the individual tree level. Next, using stable isotope ratios of soil respiration and xylem and soil water, we compare the depths of respiration to depths of water uptake to assign tree vs. understory sources of respiration. This will help

  3. Soil respiration, root biomass, and root turnover following long-term exposure of northern forests to elevated atmospheric CO2 and tropospheric O3.

    PubMed

    Pregitzer, Kurt S; Burton, Andrew J; King, John S; Zak, Donald R

    2008-01-01

    The Rhinelander free-air CO(2) enrichment (FACE) experiment is designed to understand ecosystem response to elevated atmospheric carbon dioxide (+CO(2)) and elevated tropospheric ozone (+O(3)). The objectives of this study were: to understand how soil respiration responded to the experimental treatments; to determine whether fine-root biomass was correlated to rates of soil respiration; and to measure rates of fine-root turnover in aspen (Populus tremuloides) forests and determine whether root turnover might be driving patterns in soil respiration. Soil respiration was measured, root biomass was determined, and estimates of root production, mortality and biomass turnover were made. Soil respiration was greatest in the +CO(2) and +CO(2) +O(3) treatments across all three plant communities. Soil respiration was correlated with increases in fine-root biomass. In the aspen community, annual fine-root production and mortality (g m(-2)) were positively affected by +O(3). After 10 yr of exposure, +CO(2) +O(3)-induced increases in belowground carbon allocation suggest that the positive effects of elevated CO(2) on belowground net primary productivity (NPP) may not be offset by negative effects of O(3). For the aspen community, fine-root biomass is actually stimulated by +O(3), and especially +CO(2) +O(3). PMID:18643941

  4. Satellite Remote Sensing of Pan-arctic Vegetation Productivity, Soil Respiration and net CO2 Exchange Using MODIS and AMSR-E Data

    NASA Astrophysics Data System (ADS)

    Nirala, M. L.; Heinsch, F. A.; Kimball, J. S.; Zhao, M.; Running, S.; Oechel, W.; McDonald, K.; Njoku, E.

    2005-05-01

    We have developed an approach for regional assessment and monitoring of land-atmosphere carbon dioxide (CO2) exchange, soil heterotrophic respiration (Rh) and vegetation productivity for arctic tundra using global satellite remote sensing at optical and microwave wavelengths. We use C- and X-band brightness temperatures from AMSR-E to extract surface wetness and temperature, and MODIS data to derive land cover, Leaf Area Index (LAI) and Net Primary Production (NPP) information. Calibration and validation activities involve comparisons between satellite remote sensing and tundra CO2 eddy flux tower and biophysical measurement networks and hydro-ecological process model simulations. We analyze spatial and temporal anomalies and environmental drivers of land-atmosphere net CO2 exchange at weekly and annual time steps. Surface soil moisture status and temperature as detected from satellite remote sensing observations are found to be major drivers spatial and temporal patterns of tundra net CO2 exchange and photosynthetic and respiration processes. We also find that satellite microwave measurements are capable of capturing seasonal variations and regional patterns in tundra soil heterotrophic respiration and CO2 exchange, while our ability to extract spatial patterns at the scale of surface heterogeneity is limited by the coarse spatial scale of the satellite remote sensing footprint. Our results also indicate that carbon cycle response to climate change is non-linear and strongly coupled to arctic surface hydrology. This work was performed at The University of Montana and Jet Propulsion Laboratory, California Institute of Technology, under contract with the National Aeronautics and Space Administration.

  5. After more than a decade of soil moisture deficit, tropical rainforest trees maintain photosynthetic capacity, despite increased leaf respiration.

    PubMed

    Rowland, Lucy; Lobo-do-Vale, Raquel L; Christoffersen, Bradley O; Melém, Eliane A; Kruijt, Bart; Vasconcelos, Steel S; Domingues, Tomas; Binks, Oliver J; Oliveira, Alex A R; Metcalfe, Daniel; da Costa, Antonio C L; Mencuccini, Maurizio; Meir, Patrick

    2015-12-01

    Determining climate change feedbacks from tropical rainforests requires an understanding of how carbon gain through photosynthesis and loss through respiration will be altered. One of the key changes that tropical rainforests may experience under future climate change scenarios is reduced soil moisture availability. In this study we examine if and how both leaf photosynthesis and leaf dark respiration acclimate following more than 12 years of experimental soil moisture deficit, via a through-fall exclusion experiment (TFE) in an eastern Amazonian rainforest. We find that experimentally drought-stressed trees and taxa maintain the same maximum leaf photosynthetic capacity as trees in corresponding control forest, independent of their susceptibility to drought-induced mortality. We hypothesize that photosynthetic capacity is maintained across all treatments and taxa to take advantage of short-lived periods of high moisture availability, when stomatal conductance (gs ) and photosynthesis can increase rapidly, potentially compensating for reduced assimilate supply at other times. Average leaf dark respiration (Rd ) was elevated in the TFE-treated forest trees relative to the control by 28.2 ± 2.8% (mean ± one standard error). This mean Rd value was dominated by a 48.5 ± 3.6% increase in the Rd of drought-sensitive taxa, and likely reflects the need for additional metabolic support required for stress-related repair, and hydraulic or osmotic maintenance processes. Following soil moisture deficit that is maintained for several years, our data suggest that changes in respiration drive greater shifts in the canopy carbon balance, than changes in photosynthetic capacity. PMID:26179437

  6. Microbial respiration and extracellular enzyme activity in sediments from the Gulf of Mexico hypoxic zone

    EPA Science Inventory

    This study explores the relationship between sediment chemistry (TC, TN, TP) and microbial respiration (DHA) and extracellular enzyme activity (EEA) across the Gulf of Mexico (GOM) hypoxic zone. TC, TN, and TP were all positively correlated with each other (r=0.19-0.68). DHA was ...

  7. Health Occupations--Respiration Therapy Technician. Kit No. 66. Instructor's Manual [and] Student Learning Activity Guide.

    ERIC Educational Resources Information Center

    Jackson, Janette

    An instructor's manual and student activity guide on respiration therapy technician are provided in this set of prevocational education materials which focuses on the vocational area of health occupations. (This set of materials is one of ninety-two prevocational education sets arranged around a cluster of seven vocational offerings: agriculture,…

  8. TOC, ATP AND RESPIRATION RATE AS CONTROL PARAMETERS FOR THE ACTIVATED SLUDGE PROCESS

    EPA Science Inventory

    This research was conducted to determine the feasibility of using TOC, ATP and respiration rates as tools for controlling a complete mix activated sludge plant handling a significant amount of industrial waste. Control methodology was centered on using F/M ratio which was determi...

  9. Activation of TRPV4 Regulates Respiration through Indirect Activation of Bronchopulmonary Sensory Neurons

    PubMed Central

    Gu, Qihai (David); Moss, Charles R.; Kettelhut, Kristen L.; Gilbert, Carolyn A.; Hu, Hongzhen

    2016-01-01

    Transient receptor potential vanilloid receptor 4 (TRPV4) is a calcium-permeable non-selective cation channel implicated in numerous physiological and pathological functions. This study aimed to investigate the effect of TRPV4 activation on respiration and to explore the potential involvement of bronchopulmonary sensory neurons. Potent TRPV4 agonist GSK1016790A was injected into right atrium in anesthetized spontaneously breathing rats and the changes in breathing were measured. Patch-clamp recording was performed to investigate the effect of GSK1016790A or another TRPV4 activator 4α-PDD on cultured rat vagal bronchopulmonary sensory neurons. Immunohistochemistry was carried out to determine the TRPV4-expressing cells in lung slices obtained from TRPV4-EGFP mice. Our results showed, that right-atrial injection of GSK1016790A evoked a slow-developing, long-lasting rapid shallow breathing in anesthetized rats. Activation of TRPV4 also significantly potentiated capsaicin-evoked chemoreflex responses. The alteration in ventilation induced by GSK1016790A was abolished by cutting or perineural capsaicin treatment of both vagi, indicating the involvement of bronchopulmonary afferent neurons. The stimulating and sensitizing effects of GSK1016790A were abolished by a selective TRPV4 antagonist GSK2193874 and also by inhibiting cyclooxygenase with indomethacin. Surprising, GSK1016790A or 4α-PDD did not activate isolated bronchopulmonary sensory neurons, nor did they modulate capsaicin-induced inward currents in these neurons. Furthermore, TRPV4 expression was found in alveolar macrophages, alveolar epithelial, and vascular endothelial cells. Collectively, our results suggest that GSK1016790A regulates the respiration through an indirect activation of bronchopulmonary sensory neurons, likely via its stimulation of other TRPV4-expressing cells in the lungs and airways. PMID:26973533

  10. Carbon balance of surfaces vs. ecosystems: advantages of measuring eddy covariance and soil respiration simultaneously in dry grassland ecosystems

    NASA Astrophysics Data System (ADS)

    Nagy, Z.; Pintér, K.; Pavelka, M.; Darenová, E.; Balogh, J.

    2011-02-01

    An automated open system for measurement of soil CO2 efflux (Rsc) was developed and calibrated against known fluxes and tested in the field, while measuring soil respiration also by the gradient method (Rsg) at a dry sandy grassland (Bugac, Hungary). Ecosystem respiration (Reco) was measured by the eddy covariance technique. Small chamber size (5 cm in diameter) of the chamber system made it possible to use the chambers also in vegetation gaps, thereby avoiding the necessity of removing shoots, the disturbance of the spatial structure of vegetation and the upper soil layer. Low air flow rates associated with small chamber volume and chamber design allowed the overpressure range to stabilize between 0.05-0.12 Pa. While the correlation between ecosystem and soil CO2 efflux rates as measured by the independent methods was significant, Reco rates were similar or even lower than Rsc in the low flux (up to 2 μmol CO2 m-2 s-1) range, probably due to the larger than assumed storage flux. The gradient method showed both up and downward CO2 fluxes originating from the main rooting zone after rains. Downward fluxes within the soil profile amounted to 15% of the simultaneous upward fluxes and to ~ 7.6% of the total (upward) effluxes during the 3 months study. The upper 5 cm soil layer contributed to ~ 50% of the total soil CO2 efflux. The continuously operated automatic open chamber system and the gradient system makes possible the detection of situations when the eddy system underestimates Reco, gives the lower limit of underestimation (chamber system) and helps in quantifying the downward flux component of soil respiration (gradient method) between the soil layers. These latter (downward) fluxes are expected to seriously affect (1) the Reco vs. temperature response functions and (2) the net ecosystem exchange of CO2 (NEE) vs. photon flux density response functions, therefore potentially affecting also the gap filling procedures and to led to a situation (3) when the

  11. Grazing exclusion reduced soil respiration but increased its temperature sensitivity in a Meadow Grassland on the Tibetan Plateau.

    PubMed

    Chen, Ji; Zhou, Xuhui; Wang, Junfeng; Hruska, Tracy; Shi, Weiyu; Cao, Junji; Zhang, Baocheng; Xu, Gexi; Chen, Yizhao; Luo, Yiqi

    2016-02-01

    Understanding anthropogenic influences on soil respiration (R s) is critical for accurate predictions of soil carbon fluxes, but it is not known how R s responds to grazing exclusion (GE). Here, we conducted a manipulative experiment in a meadow grassland on the Tibetan Plateau to investigate the effects of GE on R s. The exclusion of livestock significantly increased soil moisture and above-ground biomass, but it decreased soil temperature, microbial biomass carbon (MBC), and R s. Regression analysis indicated that the effects of GE on R s were mainly due to changes in soil temperature, soil moisture, and MBC. Compared with the grazed blocks, GE significantly decreased soil carbon release by 23.6% over the growing season and 21.4% annually, but it increased the temperature sensitivity (Q10) of R s by 6.5% and 14.2% for the growing season and annually respectively. Therefore, GE may reduce the release of soil carbon from the Tibetan Plateau, but under future climate warming scenarios, the increases in Q10 induced by GE could lead to increased carbon emissions. PMID:26865957

  12. Soil temperature synchronisation improves estimation of daily variation of ecosystem respiration in Sphagnum peatlands

    NASA Astrophysics Data System (ADS)

    D'Angelo, Benoît; Gogo, Sébastien; Le Moing, Franck; Jégou, Fabrice; Guimbaud, Christophe; Laggoun, Fatima

    2015-04-01

    Ecosystem respiration (ER) is a key process in the global C cycle and thus, plays an important role in the climate regulation. Peatlands contain a third of the world soil C in spite of their relatively low global area (3% of land area). Although these ecosystems represent potentially a significant source of C under global change, they are still not taken into account accordingly in global climatic models. Therefore, ER variations have to be accounted for, especially by estimating its dependence to temperature.s The relationship between ER and temperature often relies only on one soil temperature depth and the latter is generally taken in the first 10 centimetres. Previous studies showed that the temperature dependence of ER depends on the depth at which the temperature is recorded. The depth selection for temperature measurement is thus a predominant issue. A way to deal with this is to analyse the time-delay between ER and temperature. The aim of this work is to assess whether using synchronised data in models leads to a better ER daily variation estimation than using non-synchronised data. ER measurements were undertaken in 2013 in 4 Sphagnum peatlands across France: La Guette (N 47°19'44', E 2°17'04', 154m) in July, Landemarais (N 48°26'30', E -1°10'54', 145m) in August, Frasne (N 46°49'35', E 6°10'20', 836m) in September, and Bernadouze (N 42°48'09', E 1°25'24', 1500m) in October. A closed method chamber was used to measure ER hourly during 72 hours in each of the 4 replicates installed in each site. Average ER ranged from 1.75 μmol m-2 s-1 to 6.13 μmol m-2 s-1. A weather station was used to record meteorological data and soil temperature profiles (5, 10, 20 and 30 cm). Synchronised data were determined for each depth by selecting the time-delay leading to the best correlation between ER and soil temperature. The data were used to simulate ER according to commonly used equations: linear, exponential with Q10, Arrhenius, Lloyd and Taylor. Models

  13. Deconvolving temperature and substrate effects on soil heterotrophic respiration under multiple global change factors in mixed grass prairie

    NASA Astrophysics Data System (ADS)

    Tucker, C.; Nie, M.; Pendall, E. G.

    2013-12-01

    The temperature sensitivity of soil organic matter (SOM) decomposition exposed to elevated CO2 and warming represents a substantial source of uncertainty in predicting climate-carbon feedbacks. Here, we evaluated temperature responses of soil heterotrophic respiration via soil laboratory incubations at the Prairie Heating and CO2 Enrichment experiment. Soils were collected from plots with and without native vegetation so as to examine plant-mediated effects on temperature sensitivity of SOM decomposition under simulated climate change. Thus, soils were exposed to full factorial combinations of elevated CO2, warming and vegetation removal for four years prior to sampling. Incubations were conducted for 60 days at optimal water content (60% of field capacity) and 15, 22 or 30 °C. Total soil C content was measured prior to the start of incubations, and soil respiration was measured 11 times throughout the incubation. Data were analyzed in the context of a Bayesian model where respiration of the fast (aka ';labile') and slow (aka ';recalcitrant') soil C pools were determined by separate Arrhenius-type temperature sensitivity functions as well as by the pool size. We tested competing hypotheses that differences in soil heterotrophic respiration under the different treatments could be explained by 1) changes in the exponential temperature sensitivity (Q10), 2) changes in the base rate, or 3) changes in the size of the fast and slow pools. The model predictions fit the observed data well (r2 = 0.93) across all treatments. The Q10 of both the fast and slow pools decreased ~40% between the 15 and 30 °C incubation temperature across all treatment levels. The Q10 of the fast pool was lower in the warmed treatment than the control in both fallow and vegetated soils, consistent with thermal acclimation. The Q10 of the fast pool under elevated CO2 and warming was lowest in the fallow soil, but highest in the vegetated soil. This indicates that rhizosphere priming plays a role

  14. A STUDY OF NITRATE RESPIRATION IN THE ACTIVATED SLUDGE PROCESS

    EPA Science Inventory

    In an experimental, 570-cum/day (0.15-mgd) activated sludge plant treating domestic wastewater from a correctional facility, 76 to 87 percent nitrogen removal was obtained via sludge synthesis and biological denitrification using endogenous H-donors in a compartmentalized reactor...

  15. The two Drosophila cytochrome C proteins can function in both respiration and caspase activation

    PubMed Central

    Arama, Eli; Bader, Maya; Srivastava, Mayank; Bergmann, Andreas; Steller, Hermann

    2006-01-01

    Cytochrome C has two apparently separable cellular functions: respiration and caspase activation during apoptosis. While a role of the mitochondria and cytochrome C in the assembly of the apoptosome and caspase activation has been established for mammalian cells, the existence of a comparable function for cytochrome C in invertebrates remains controversial. Drosophila possesses two cytochrome c genes, cyt-c-d and cyt-c-p. We show tha