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Sample records for adapter mda flight

  1. Adaptive nonlinear flight control

    NASA Astrophysics Data System (ADS)

    Rysdyk, Rolf Theoduor

    1998-08-01

    Research under supervision of Dr. Calise and Dr. Prasad at the Georgia Institute of Technology, School of Aerospace Engineering. has demonstrated the applicability of an adaptive controller architecture. The architecture successfully combines model inversion control with adaptive neural network (NN) compensation to cancel the inversion error. The tiltrotor aircraft provides a specifically interesting control design challenge. The tiltrotor aircraft is capable of converting from stable responsive fixed wing flight to unstable sluggish hover in helicopter configuration. It is desirable to provide the pilot with consistency in handling qualities through a conversion from fixed wing flight to hover. The linear model inversion architecture was adapted by providing frequency separation in the command filter and the error-dynamics, while not exiting the actuator modes. This design of the architecture provides for a model following setup with guaranteed performance. This in turn allowed for convenient implementation of guaranteed handling qualities. A rigorous proof of boundedness is presented making use of compact sets and the LaSalle-Yoshizawa theorem. The analysis allows for the addition of the e-modification which guarantees boundedness of the NN weights in the absence of persistent excitation. The controller is demonstrated on the Generic Tiltrotor Simulator of Bell-Textron and NASA Ames R.C. The model inversion implementation is robustified with respect to unmodeled input dynamics, by adding dynamic nonlinear damping. A proof of boundedness of signals in the system is included. The effectiveness of the robustification is also demonstrated on the XV-15 tiltrotor. The SHL Perceptron NN provides a more powerful application, based on the universal approximation property of this type of NN. The SHL NN based architecture is also robustified with the dynamic nonlinear damping. A proof of boundedness extends the SHL NN augmentation with robustness to unmodeled actuator

  2. Digital adaptive flight controller development

    NASA Technical Reports Server (NTRS)

    Kaufman, H.; Alag, G.; Berry, P.; Kotob, S.

    1974-01-01

    A design study of adaptive control logic suitable for implementation in modern airborne digital flight computers was conducted. Two designs are described for an example aircraft. Each of these designs uses a weighted least squares procedure to identify parameters defining the dynamics of the aircraft. The two designs differ in the way in which control law parameters are determined. One uses the solution of an optimal linear regulator problem to determine these parameters while the other uses a procedure called single stage optimization. Extensive simulation results and analysis leading to the designs are presented.

  3. Flight Approach to Adaptive Control Research

    NASA Technical Reports Server (NTRS)

    Pavlock, Kate Maureen; Less, James L.; Larson, David Nils

    2011-01-01

    The National Aeronautics and Space Administration's Dryden Flight Research Center completed flight testing of adaptive controls research on a full-scale F-18 testbed. The testbed served as a full-scale vehicle to test and validate adaptive flight control research addressing technical challenges involved with reducing risk to enable safe flight in the presence of adverse conditions such as structural damage or control surface failures. This paper describes the research interface architecture, risk mitigations, flight test approach and lessons learned of adaptive controls research.

  4. Flight Test Approach to Adaptive Control Research

    NASA Technical Reports Server (NTRS)

    Pavlock, Kate Maureen; Less, James L.; Larson, David Nils

    2011-01-01

    The National Aeronautics and Space Administration s Dryden Flight Research Center completed flight testing of adaptive controls research on a full-scale F-18 testbed. The validation of adaptive controls has the potential to enhance safety in the presence of adverse conditions such as structural damage or control surface failures. This paper describes the research interface architecture, risk mitigations, flight test approach and lessons learned of adaptive controls research.

  5. Hybrid Adaptive Flight Control with Model Inversion Adaptation

    NASA Technical Reports Server (NTRS)

    Nguyen, Nhan

    2011-01-01

    This study investigates a hybrid adaptive flight control method as a design possibility for a flight control system that can enable an effective adaptation strategy to deal with off-nominal flight conditions. The hybrid adaptive control blends both direct and indirect adaptive control in a model inversion flight control architecture. The blending of both direct and indirect adaptive control provides a much more flexible and effective adaptive flight control architecture than that with either direct or indirect adaptive control alone. The indirect adaptive control is used to update the model inversion controller by an on-line parameter estimation of uncertain plant dynamics based on two methods. The first parameter estimation method is an indirect adaptive law based on the Lyapunov theory, and the second method is a recursive least-squares indirect adaptive law. The model inversion controller is therefore made to adapt to changes in the plant dynamics due to uncertainty. As a result, the modeling error is reduced that directly leads to a decrease in the tracking error. In conjunction with the indirect adaptive control that updates the model inversion controller, a direct adaptive control is implemented as an augmented command to further reduce any residual tracking error that is not entirely eliminated by the indirect adaptive control.

  6. Physiological adaptation to space flight

    NASA Technical Reports Server (NTRS)

    Nicogossian, Arnauld E.; Sulzman, Frank M.; Gaiser, Karen K.; Teeter, Ronald C.

    1990-01-01

    In space, adaptive physiological changes have been observed in virtually all body systems, but how far these changes progress with time is not known. Their time course demonstrates variable patterns; some systems show evidence of gradual and progressive change. Biomedical postflight data have shown that a compensatory period of readaptation to one gravity is required after space flight, with longer intervals required for longer missions. Consistent readaptation trends include orthostatic intolerance and neurovestibular difficulties. For the long-duration missions of the exploration era, it is critical to determine the extent to which deleterious changes (e.g., bone loss and possible immunological changes) can be reversed upon return to earth. Radiation protection is another critical enabling element for missions beyond low earth orbit. Radiation exposure guidelines have not been established for exploration missions. Currently our experience is insufficient to prescribe countermeasures for the stay times associated with a lunar base or a mission to Mars. Artificial gravity may provide a solution, but the level and duration of exposure necessary to prevent deconditioning must be determined. Central issues for medical care in remote settings are preventive, diagnostic, and therapeutic care and the minimization of risk.

  7. Rotationally Adaptive Flight Test Surface

    NASA Technical Reports Server (NTRS)

    Barrett, Ron

    1999-01-01

    Research on a new design of flutter exciter vane using adaptive materials was conducted. This novel design is based on all-moving aerodynamic surface technology and consists of a structurally stiff main spar, a series of piezoelectric actuator elements and an aerodynamic shell which is pivoted around the main spar. The work was built upon the current missile-type all-moving surface designs and change them so they are better suited for flutter excitation through the transonic flight regime. The first portion of research will be centered on aerodynamic and structural modeling of the system. USAF DatCom and vortex lattice codes was used to capture the fundamental aerodynamics of the vane. Finite element codes and laminated plate theory and virtual work analyses will be used to structurally model the aerodynamic vane and wing tip. Following the basic modeling, a flutter test vane was designed. Each component within the structure was designed to meet the design loads. After the design loads are met, then the deflections will be maximized and the internal structure will be laid out. In addition to the structure, a basic electrical control network will be designed which will be capable of driving a scaled exciter vane. The third and final stage of main investigation involved the fabrication of a 1/4 scale vane. This scaled vane was used to verify kinematics and structural mechanics theories on all-moving actuation. Following assembly, a series of bench tests was conducted to determine frequency response, electrical characteristics, mechanical and kinematic properties. Test results indicate peak-to-peak deflections of 1.1 deg with a corner frequency of just over 130 Hz.

  8. Research in digital adaptive flight controllers

    NASA Technical Reports Server (NTRS)

    Kaufman, H.

    1976-01-01

    A design study of adaptive control logic suitable for implementation in modern airborne digital flight computers was conducted. Both explicit controllers which directly utilize parameter identification and implicit controllers which do not require identification were considered. Extensive analytical and simulation efforts resulted in the recommendation of two explicit digital adaptive flight controllers. Interface weighted least squares estimation procedures with control logic were developed using either optimal regulator theory or with control logic based upon single stage performance indices.

  9. Adaptive Flight Control Research at NASA

    NASA Technical Reports Server (NTRS)

    Motter, Mark A.

    2008-01-01

    A broad overview of current adaptive flight control research efforts at NASA is presented, as well as some more detailed discussion of selected specific approaches. The stated objective of the Integrated Resilient Aircraft Control Project, one of NASA s Aviation Safety programs, is to advance the state-of-the-art of adaptive controls as a design option to provide enhanced stability and maneuverability margins for safe landing in the presence of adverse conditions such as actuator or sensor failures. Under this project, a number of adaptive control approaches are being pursued, including neural networks and multiple models. Validation of all the adaptive control approaches will use not only traditional methods such as simulation, wind tunnel testing and manned flight tests, but will be augmented with recently developed capabilities in unmanned flight testing.

  10. Adaptive Flight Control for Aircraft Safety Enhancements

    NASA Technical Reports Server (NTRS)

    Nguyen, Nhan T.; Gregory, Irene M.; Joshi, Suresh M.

    2008-01-01

    This poster presents the current adaptive control research being conducted at NASA ARC and LaRC in support of the Integrated Resilient Aircraft Control (IRAC) project. The technique "Approximate Stability Margin Analysis of Hybrid Direct-Indirect Adaptive Control" has been developed at NASA ARC to address the needs for stability margin metrics for adaptive control that potentially enables future V&V of adaptive systems. The technique "Direct Adaptive Control With Unknown Actuator Failures" is developed at NASA LaRC to deal with unknown actuator failures. The technique "Adaptive Control with Adaptive Pilot Element" is being researched at NASA LaRC to investigate the effects of pilot interactions with adaptive flight control that can have implications of stability and performance.

  11. L(sub 1) Adaptive Flight Control System: Flight Evaluation and Technology Transition

    NASA Technical Reports Server (NTRS)

    Xargay, Enric; Hovakimyan, Naira; Dobrokhodov, Vladimir; Kaminer, Isaac; Gregory, Irene M.; Cao, Chengyu

    2010-01-01

    Certification of adaptive control technologies for both manned and unmanned aircraft represent a major challenge for current Verification and Validation techniques. A (missing) key step towards flight certification of adaptive flight control systems is the definition and development of analysis tools and methods to support Verification and Validation for nonlinear systems, similar to the procedures currently used for linear systems. In this paper, we describe and demonstrate the advantages of L(sub l) adaptive control architectures for closing some of the gaps in certification of adaptive flight control systems, which may facilitate the transition of adaptive control into military and commercial aerospace applications. As illustrative examples, we present the results of a piloted simulation evaluation on the NASA AirSTAR flight test vehicle, and results of an extensive flight test program conducted by the Naval Postgraduate School to demonstrate the advantages of L(sub l) adaptive control as a verifiable robust adaptive flight control system.

  12. F-8C adaptive flight control laws

    NASA Technical Reports Server (NTRS)

    Hartmann, G. L.; Harvey, C. A.; Stein, G.; Carlson, D. N.; Hendrick, R. C.

    1977-01-01

    Three candidate digital adaptive control laws were designed for NASA's F-8C digital flyby wire aircraft. Each design used the same control laws but adjusted the gains with a different adaptative algorithm. The three adaptive concepts were: high-gain limit cycle, Liapunov-stable model tracking, and maximum likelihood estimation. Sensors were restricted to conventional inertial instruments (rate gyros and accelerometers) without use of air-data measurements. Performance, growth potential, and computer requirements were used as criteria for selecting the most promising of these candidates for further refinement. The maximum likelihood concept was selected primarily because it offers the greatest potential for identifying several aircraft parameters and hence for improved control performance in future aircraft application. In terms of identification and gain adjustment accuracy, the MLE design is slightly superior to the other two, but this has no significant effects on the control performance achievable with the F-8C aircraft. The maximum likelihood design is recommended for flight test, and several refinements to that design are proposed.

  13. HIDEC F-15 adaptive engine control system flight test results

    NASA Technical Reports Server (NTRS)

    Smolka, James W.

    1987-01-01

    NASA-Ames' Highly Integrated Digital Electronic Control (HIDEC) flight test program aims to develop fully integrated airframe, propulsion, and flight control systems. The HIDEC F-15 adaptive engine control system flight test program has demonstrated that significant performance improvements are obtainable through the retention of stall-free engine operation throughout the aircraft flight and maneuver envelopes. The greatest thrust increase was projected for the medium-to-high altitude flight regime at subsonic speed which is of such importance to air combat. Adaptive engine control systems such as the HIDEC F-15's can be used to upgrade the performance of existing aircraft without resort to expensive reengining programs.

  14. Full-Scale Flight Research Testbeds: Adaptive and Intelligent Control

    NASA Technical Reports Server (NTRS)

    Pahle, Joe W.

    2008-01-01

    This viewgraph presentation describes the adaptive and intelligent control methods used for aircraft survival. The contents include: 1) Motivation for Adaptive Control; 2) Integrated Resilient Aircraft Control Project; 3) Full-scale Flight Assets in Use for IRAC; 4) NASA NF-15B Tail Number 837; 5) Gen II Direct Adaptive Control Architecture; 6) Limited Authority System; and 7) 837 Flight Experiments. A simulated destabilization failure analysis along with experience and lessons learned are also presented.

  15. Behavioural adaptations to flight into thin air

    PubMed Central

    Weinzierl, Rolf

    2016-01-01

    Soaring raptors can fly at high altitudes of up to 9000 m. The behavioural adjustments to high-altitude flights are largely unknown. We studied thermalling flights of Himalayan vultures (Gyps himalayensis) from 50 to 6500 m above sea level, a twofold range of air densities. To create the necessary lift to support the same weight and maintain soaring flight in thin air birds might modify lift coefficient by biophysical changes, such as wing posture and increasing the power expenditure. Alternatively, they can change their flight characteristics. We show that vultures use the latter and increase circle radius by 35% and airspeed by 21% over their flight altitude range. These simple behavioural adjustments enable vultures to move seamlessly during their annual migrations over the Himalaya without increasing energy output for flight at high elevations. PMID:28120805

  16. Behavioural adaptations to flight into thin air.

    PubMed

    Sherub, Sherub; Bohrer, Gil; Wikelski, Martin; Weinzierl, Rolf

    2016-10-01

    Soaring raptors can fly at high altitudes of up to 9000 m. The behavioural adjustments to high-altitude flights are largely unknown. We studied thermalling flights of Himalayan vultures (Gyps himalayensis) from 50 to 6500 m above sea level, a twofold range of air densities. To create the necessary lift to support the same weight and maintain soaring flight in thin air birds might modify lift coefficient by biophysical changes, such as wing posture and increasing the power expenditure. Alternatively, they can change their flight characteristics. We show that vultures use the latter and increase circle radius by 35% and airspeed by 21% over their flight altitude range. These simple behavioural adjustments enable vultures to move seamlessly during their annual migrations over the Himalaya without increasing energy output for flight at high elevations.

  17. Closing the Certification Gaps in Adaptive Flight Control Software

    NASA Technical Reports Server (NTRS)

    Jacklin, Stephen A.

    2008-01-01

    Over the last five decades, extensive research has been performed to design and develop adaptive control systems for aerospace systems and other applications where the capability to change controller behavior at different operating conditions is highly desirable. Although adaptive flight control has been partially implemented through the use of gain-scheduled control, truly adaptive control systems using learning algorithms and on-line system identification methods have not seen commercial deployment. The reason is that the certification process for adaptive flight control software for use in national air space has not yet been decided. The purpose of this paper is to examine the gaps between the state-of-the-art methodologies used to certify conventional (i.e., non-adaptive) flight control system software and what will likely to be needed to satisfy FAA airworthiness requirements. These gaps include the lack of a certification plan or process guide, the need to develop verification and validation tools and methodologies to analyze adaptive controller stability and convergence, as well as the development of metrics to evaluate adaptive controller performance at off-nominal flight conditions. This paper presents the major certification gap areas, a description of the current state of the verification methodologies, and what further research efforts will likely be needed to close the gaps remaining in current certification practices. It is envisioned that closing the gap will require certain advances in simulation methods, comprehensive methods to determine learning algorithm stability and convergence rates, the development of performance metrics for adaptive controllers, the application of formal software assurance methods, the application of on-line software monitoring tools for adaptive controller health assessment, and the development of a certification case for adaptive system safety of flight.

  18. Hybrid adaptive ascent flight control for a flexible launch vehicle

    NASA Astrophysics Data System (ADS)

    Lefevre, Brian D.

    For the purpose of maintaining dynamic stability and improving guidance command tracking performance under off-nominal flight conditions, a hybrid adaptive control scheme is selected and modified for use as a launch vehicle flight controller. This architecture merges a model reference adaptive approach, which utilizes both direct and indirect adaptive elements, with a classical dynamic inversion controller. This structure is chosen for a number of reasons: the properties of the reference model can be easily adjusted to tune the desired handling qualities of the spacecraft, the indirect adaptive element (which consists of an online parameter identification algorithm) continually refines the estimates of the evolving characteristic parameters utilized in the dynamic inversion, and the direct adaptive element (which consists of a neural network) augments the linear feedback signal to compensate for any nonlinearities in the vehicle dynamics. The combination of these elements enables the control system to retain the nonlinear capabilities of an adaptive network while relying heavily on the linear portion of the feedback signal to dictate the dynamic response under most operating conditions. To begin the analysis, the ascent dynamics of a launch vehicle with a single 1st stage rocket motor (typical of the Ares 1 spacecraft) are characterized. The dynamics are then linearized with assumptions that are appropriate for a launch vehicle, so that the resulting equations may be inverted by the flight controller in order to compute the control signals necessary to generate the desired response from the vehicle. Next, the development of the hybrid adaptive launch vehicle ascent flight control architecture is discussed in detail. Alterations of the generic hybrid adaptive control architecture include the incorporation of a command conversion operation which transforms guidance input from quaternion form (as provided by NASA) to the body-fixed angular rate commands needed by the

  19. Methodologies for Adaptive Flight Envelope Estimation and Protection

    NASA Technical Reports Server (NTRS)

    Tang, Liang; Roemer, Michael; Ge, Jianhua; Crassidis, Agamemnon; Prasad, J. V. R.; Belcastro, Christine

    2009-01-01

    This paper reports the latest development of several techniques for adaptive flight envelope estimation and protection system for aircraft under damage upset conditions. Through the integration of advanced fault detection algorithms, real-time system identification of the damage/faulted aircraft and flight envelop estimation, real-time decision support can be executed autonomously for improving damage tolerance and flight recoverability. Particularly, a bank of adaptive nonlinear fault detection and isolation estimators were developed for flight control actuator faults; a real-time system identification method was developed for assessing the dynamics and performance limitation of impaired aircraft; online learning neural networks were used to approximate selected aircraft dynamics which were then inverted to estimate command margins. As off-line training of network weights is not required, the method has the advantage of adapting to varying flight conditions and different vehicle configurations. The key benefit of the envelope estimation and protection system is that it allows the aircraft to fly close to its limit boundary by constantly updating the controller command limits during flight. The developed techniques were demonstrated on NASA s Generic Transport Model (GTM) simulation environments with simulated actuator faults. Simulation results and remarks on future work are presented.

  20. Flight Test of L1 Adaptive Control Law: Offset Landings and Large Flight Envelope Modeling Work

    NASA Technical Reports Server (NTRS)

    Gregory, Irene M.; Xargay, Enric; Cao, Chengyu; Hovakimyan, Naira

    2011-01-01

    This paper presents new results of a flight test of the L1 adaptive control architecture designed to directly compensate for significant uncertain cross-coupling in nonlinear systems. The flight test was conducted on the subscale turbine powered Generic Transport Model that is an integral part of the Airborne Subscale Transport Aircraft Research system at the NASA Langley Research Center. The results presented include control law evaluation for piloted offset landing tasks as well as results in support of nonlinear aerodynamic modeling and real-time dynamic modeling of the departure-prone edges of the flight envelope.

  1. Adaptive Trajectory Prediction Algorithm for Climbing Flights

    NASA Technical Reports Server (NTRS)

    Schultz, Charles Alexander; Thipphavong, David P.; Erzberger, Heinz

    2012-01-01

    Aircraft climb trajectories are difficult to predict, and large errors in these predictions reduce the potential operational benefits of some advanced features for NextGen. The algorithm described in this paper improves climb trajectory prediction accuracy by adjusting trajectory predictions based on observed track data. It utilizes rate-of-climb and airspeed measurements derived from position data to dynamically adjust the aircraft weight modeled for trajectory predictions. In simulations with weight uncertainty, the algorithm is able to adapt to within 3 percent of the actual gross weight within two minutes of the initial adaptation. The root-mean-square of altitude errors for five-minute predictions was reduced by 73 percent. Conflict detection performance also improved, with a 15 percent reduction in missed alerts and a 10 percent reduction in false alerts. In a simulation with climb speed capture intent and weight uncertainty, the algorithm improved climb trajectory prediction accuracy by up to 30 percent and conflict detection performance, reducing missed and false alerts by up to 10 percent.

  2. Optimizing aircraft performance with adaptive, integrated flight/propulsion control

    NASA Technical Reports Server (NTRS)

    Smith, R. H.; Chisholm, J. D.; Stewart, J. F.

    1991-01-01

    The Performance-Seeking Control (PSC) integrated flight/propulsion adaptive control algorithm presented was developed in order to optimize total aircraft performance during steady-state engine operation. The PSC multimode algorithm minimizes fuel consumption at cruise conditions, while maximizing excess thrust during aircraft accelerations, climbs, and dashes, and simultaneously extending engine service life through reduction of fan-driving turbine inlet temperature upon engagement of the extended-life mode. The engine models incorporated by the PSC are continually upgraded, using a Kalman filter to detect anomalous operations. The PSC algorithm will be flight-demonstrated by an F-15 at NASA-Dryden.

  3. Stability and Performance Metrics for Adaptive Flight Control

    NASA Technical Reports Server (NTRS)

    Stepanyan, Vahram; Krishnakumar, Kalmanje; Nguyen, Nhan; VanEykeren, Luarens

    2009-01-01

    This paper addresses the problem of verifying adaptive control techniques for enabling safe flight in the presence of adverse conditions. Since the adaptive systems are non-linear by design, the existing control verification metrics are not applicable to adaptive controllers. Moreover, these systems are in general highly uncertain. Hence, the system's characteristics cannot be evaluated by relying on the available dynamical models. This necessitates the development of control verification metrics based on the system's input-output information. For this point of view, a set of metrics is introduced that compares the uncertain aircraft's input-output behavior under the action of an adaptive controller to that of a closed-loop linear reference model to be followed by the aircraft. This reference model is constructed for each specific maneuver using the exact aerodynamic and mass properties of the aircraft to meet the stability and performance requirements commonly accepted in flight control. The proposed metrics are unified in the sense that they are model independent and not restricted to any specific adaptive control methods. As an example, we present simulation results for a wing damaged generic transport aircraft with several existing adaptive controllers.

  4. Multiple Docking Adapter Illustration

    NASA Technical Reports Server (NTRS)

    1972-01-01

    This cutaway drawing details the major characteristics of the Skylab Multiple Docking Adapter (MDA). The MDA, built under the direction of the Marshall Space Flight Center, housed the control units for the Apollo Telescope Mount (ATM), Earth Resources Experiment Package (EREP), and Zero-Gravity Materials Processing Facility, and provided a docking port for the Apollo Command Module (CM).

  5. Adaptive integral dynamic surface control of a hypersonic flight vehicle

    NASA Astrophysics Data System (ADS)

    Aslam Butt, Waseem; Yan, Lin; Amezquita S., Kendrick

    2015-07-01

    In this article, non-linear adaptive dynamic surface air speed and flight path angle control designs are presented for the longitudinal dynamics of a flexible hypersonic flight vehicle. The tracking performance of the control design is enhanced by introducing a novel integral term that caters to avoiding a large initial control signal. To ensure feasibility, the design scheme incorporates magnitude and rate constraints on the actuator commands. The uncertain non-linear functions are approximated by an efficient use of the neural networks to reduce the computational load. A detailed stability analysis shows that all closed-loop signals are uniformly ultimately bounded and the ? tracking performance is guaranteed. The robustness of the design scheme is verified through numerical simulations of the flexible flight vehicle model.

  6. Flight data processing with the F-8 adaptive algorithm

    NASA Technical Reports Server (NTRS)

    Hartmann, G.; Stein, G.; Petersen, K.

    1977-01-01

    An explicit adaptive control algorithm based on maximum likelihood estimation of parameters has been designed for NASA's DFBW F-8 aircraft. To avoid iterative calculations, the algorithm uses parallel channels of Kalman filters operating at fixed locations in parameter space. This algorithm has been implemented in NASA/DFRC's Remotely Augmented Vehicle (RAV) facility. Real-time sensor outputs (rate gyro, accelerometer and surface position) are telemetered to a ground computer which sends new gain values to an on-board system. Ground test data and flight records were used to establish design values of noise statistics and to verify the ground-based adaptive software. The software and its performance evaluation based on flight data are described

  7. Parameter Estimation for a Hybrid Adaptive Flight Controller

    NASA Technical Reports Server (NTRS)

    Campbell, Stefan F.; Nguyen, Nhan T.; Kaneshige, John; Krishnakumar, Kalmanje

    2009-01-01

    This paper expands on the hybrid control architecture developed at the NASA Ames Research Center by addressing issues related to indirect adaptation using the recursive least squares (RLS) algorithm. Specifically, the hybrid control architecture is an adaptive flight controller that features both direct and indirect adaptation techniques. This paper will focus almost exclusively on the modifications necessary to achieve quality indirect adaptive control. Additionally this paper will present results that, using a full non -linear aircraft model, demonstrate the effectiveness of the hybrid control architecture given drastic changes in an aircraft s dynamics. Throughout the development of this topic, a thorough discussion of the RLS algorithm as a system identification technique will be provided along with results from seven well-known modifications to the popular RLS algorithm.

  8. Context-specific adaptation of saccade gain in parabolic flight

    NASA Technical Reports Server (NTRS)

    Shelhamer, Mark; Clendaniel, Richard A.; Roberts, Dale C.

    2002-01-01

    Previous studies established that vestibular reflexes can have two adapted states (e.g., gains) simultaneously, and that a context cue (e.g., vertical eye position) can switch between the two states. Our earlier work demonstrated this phenomenon of context-specific adaptation for saccadic eye movements: we asked for gain decrease in one context state and gain increase in another context state, and then determined if a change in the context state would invoke switching between the adapted states. Horizontal and vertical eye position and head orientation could serve, to varying degrees, as cues for switching between two different saccade gains. In the present study, we asked whether gravity magnitude could serve as a context cue: saccade adaptation was performed during parabolic flight, which provides alternating levels of gravitoinertial force (0 g and 1.8 g). Results were less robust than those from ground experiments, but established that different saccade magnitudes could be associated with different gravity levels.

  9. Flight Results of the NF-15B Intelligent Flight Control System (IFCS) Aircraft with Adaptation to a Longitudinally Destabilized Plant

    NASA Technical Reports Server (NTRS)

    Bosworth, John T.

    2008-01-01

    Adaptive flight control systems have the potential to be resilient to extreme changes in airplane behavior. Extreme changes could be a result of a system failure or of damage to the airplane. The goal for the adaptive system is to provide an increase in survivability in the event that these extreme changes occur. A direct adaptive neural-network-based flight control system was developed for the National Aeronautics and Space Administration NF-15B Intelligent Flight Control System airplane. The adaptive element was incorporated into a dynamic inversion controller with explicit reference model-following. As a test the system was subjected to an abrupt change in plant stability simulating a destabilizing failure. Flight evaluations were performed with and without neural network adaptation. The results of these flight tests are presented. Comparison with simulation predictions and analysis of the performance of the adaptation system are discussed. The performance of the adaptation system is assessed in terms of its ability to stabilize the vehicle and reestablish good onboard reference model-following. Flight evaluation with the simulated destabilizing failure and adaptation engaged showed improvement in the vehicle stability margins. The convergent properties of this initial system warrant additional improvement since continued maneuvering caused continued adaptation change. Compared to the non-adaptive system the adaptive system provided better closed-loop behavior with improved matching of the onboard reference model. A detailed discussion of the flight results is presented.

  10. Astronaut adaptation to 1 G following long duration space flight

    NASA Technical Reports Server (NTRS)

    Walker, John; Greenisen, Michael; Cowell, Lynda L.; Squires, William G.

    1991-01-01

    The paper reviews the results of studies of changes undergone by several physiological systems (including the cardiovascular system, the fluid and electrolyte characteristics, the red blood cells, the bone and the muscle tissues, and the exercise capacity) due to the exposures to microgravity and to the adaptation to 1 G after a long-duration space flight. Special attention is given to the effects of various training protocols and countermeasures used to attenuate the physiological problems encountered upon return from space.

  11. Flight Validation of a Metrics Driven L(sub 1) Adaptive Control

    NASA Technical Reports Server (NTRS)

    Dobrokhodov, Vladimir; Kitsios, Ioannis; Kaminer, Isaac; Jones, Kevin D.; Xargay, Enric; Hovakimyan, Naira; Cao, Chengyu; Lizarraga, Mariano I.; Gregory, Irene M.

    2008-01-01

    The paper addresses initial steps involved in the development and flight implementation of new metrics driven L1 adaptive flight control system. The work concentrates on (i) definition of appropriate control driven metrics that account for the control surface failures; (ii) tailoring recently developed L1 adaptive controller to the design of adaptive flight control systems that explicitly address these metrics in the presence of control surface failures and dynamic changes under adverse flight conditions; (iii) development of a flight control system for implementation of the resulting algorithms onboard of small UAV; and (iv) conducting a comprehensive flight test program that demonstrates performance of the developed adaptive control algorithms in the presence of failures. As the initial milestone the paper concentrates on the adaptive flight system setup and initial efforts addressing the ability of a commercial off-the-shelf AP with and without adaptive augmentation to recover from control surface failures.

  12. Nutrition and human physiological adaptations to space flight

    NASA Technical Reports Server (NTRS)

    Lane, H. W.; LeBlanc, A. D.; Putcha, L.; Whitson, P. A.

    1993-01-01

    Space flight provides a model for the study of healthy individuals undergoing unique stresses. This review focuses on how physiological adaptations to weightlessness may affect nutrient and food requirements in space. These adaptations include reductions in body water and plasma volume, which affect the renal and cardiovascular systems and thereby fluid and electrolyte requirements. Changes in muscle mass and function may affect requirements for energy, protein and amino acids. Changes in bone mass lead to increased urinary calcium concentrations, which may increase the risk of forming renal stones. Space motion sickness may influence putative changes in gastro-intestinal-hepatic function; neurosensory alterations may affect smell and taste. Some or all of these effects may be ameliorated through the use of specially designed dietary countermeasures.

  13. Flight control system development and flight test experience with the F-111 mission adaptive wing aircraft

    NASA Technical Reports Server (NTRS)

    Larson, R. R.

    1986-01-01

    The wing on the NASA F-111 transonic aircraft technology airplane was modified to provide flexible leading and trailing edge flaps. This wing is known as the mission adaptive wing (MAW) because aerodynamic efficiency can be maintained at all speeds. Unlike a conventional wing, the MAW has no spoilers, external flap hinges, or fairings to break the smooth contour. The leading edge flaps and three-segment trailing edge flaps are controlled by a redundant fly-by-wire control system that features a dual digital primary system architecture providing roll and symmetric commands to the MAW control surfaces. A segregated analog backup system is provided in the event of a primary system failure. This paper discusses the design, development, testing, qualification, and flight test experience of the MAW primary and backup flight control systems.

  14. Flight Research into Simple Adaptive Control on the NASA FAST Aircraft

    NASA Technical Reports Server (NTRS)

    Hanson, Curtis E.

    2011-01-01

    A series of simple adaptive controllers with varying levels of complexity were designed, implemented and flight tested on the NASA Full-Scale Advanced Systems Testbed (FAST) aircraft. Lessons learned from the development and flight testing are presented.

  15. Launch Vehicle Manual Steering with Adaptive Augmenting Control In-flight Evaluations Using a Piloted Aircraft

    NASA Technical Reports Server (NTRS)

    Hanson, Curt

    2014-01-01

    An adaptive augmenting control algorithm for the Space Launch System has been developed at the Marshall Space Flight Center as part of the launch vehicles baseline flight control system. A prototype version of the SLS flight control software was hosted on a piloted aircraft at the Armstrong Flight Research Center to demonstrate the adaptive controller on a full-scale realistic application in a relevant flight environment. Concerns regarding adverse interactions between the adaptive controller and a proposed manual steering mode were investigated by giving the pilot trajectory deviation cues and pitch rate command authority.

  16. Flight Test of an L(sub 1) Adaptive Controller on the NASA AirSTAR Flight Test Vehicle

    NASA Technical Reports Server (NTRS)

    Gregory, Irene M.; Xargay, Enric; Cao, Chengyu; Hovakimyan, Naira

    2010-01-01

    This paper presents results of a flight test of the L-1 adaptive control architecture designed to directly compensate for significant uncertain cross-coupling in nonlinear systems. The flight test was conducted on the subscale turbine powered Generic Transport Model that is an integral part of the Airborne Subscale Transport Aircraft Research system at the NASA Langley Research Center. The results presented are for piloted tasks performed during the flight test.

  17. Ambiguous Tilt and Translation Motion Cues after Space Flight and Otolith Assessment during Post-Flight Re-Adaptation

    NASA Technical Reports Server (NTRS)

    Wood, Scott J.; Clarke, A. H.; Harm, D. L.; Rupert, A. H.; Clement, G. R.

    2009-01-01

    Adaptive changes during space flight in how the brain integrates vestibular cues with other sensory information can lead to impaired movement coordination, vertigo, spatial disorientation and perceptual illusions following Gtransitions. These studies are designed to examine both the physiological basis and operational implications for disorientation and tilt-translation disturbances following short duration space flights.

  18. The Role of Adaptive Supplemental Visual Cuing in Flight Simulation.

    DTIC Science & Technology

    1987-01-01

    aircrif!t S-1 a3o em J) e by in .re vi Aer" "yne foris eoo -., Doto f the a .ro raf t before learnin of 1!; 3p~ 1 1 e t a 3K, part ic,:larly perceptual...77-A195 932 THE ROLE OF ADAPTIVE SUPPLEMENTAL VISUAL CUING IN t/1 FLIGHT 5IMULRTION(U) AIR FORCE INST OF TECH WRIGHT-PATTERSONAFA OH E RBBILLMAN 1987...UNCLASSIFIED AF T/CI/NR-8 -iB6T F/G 5/9 UL E ,7hEcE7hEhhhhEEohEohhE EhhhhmmhhohhE _ _ , -,,.ah. .- ’-, - .. - ’ . ’ -~ ..V .. _ .. ,,. . . , ,,VW Y W

  19. Experimental Validation of L1 Adaptive Control: Rohrs' Counterexample in Flight

    NASA Technical Reports Server (NTRS)

    Xargay, Enric; Hovakimyan, Naira; Dobrokhodov, Vladimir; Kaminer, Issac; Kitsios, Ioannis; Cao, Chengyu; Gregory, Irene M.; Valavani, Lena

    2010-01-01

    The paper presents new results on the verification and in-flight validation of an L1 adaptive flight control system, and proposes a general methodology for verification and validation of adaptive flight control algorithms. The proposed framework is based on Rohrs counterexample, a benchmark problem presented in the early 80s to show the limitations of adaptive controllers developed at that time. In this paper, the framework is used to evaluate the performance and robustness characteristics of an L1 adaptive control augmentation loop implemented onboard a small unmanned aerial vehicle. Hardware-in-the-loop simulations and flight test results confirm the ability of the L1 adaptive controller to maintain stability and predictable performance of the closed loop adaptive system in the presence of general (artificially injected) unmodeled dynamics. The results demonstrate the advantages of L1 adaptive control as a verifiable robust adaptive control architecture with the potential of reducing flight control design costs and facilitating the transition of adaptive control into advanced flight control systems.

  20. Verification and Validation of Adaptive and Intelligent Systems with Flight Test Results

    NASA Technical Reports Server (NTRS)

    Burken, John J.; Larson, Richard R.

    2009-01-01

    F-15 IFCS project goals are: a) Demonstrate Control Approaches that can Efficiently Optimize Aircraft Performance in both Normal and Failure Conditions [A] & [B] failures. b) Advance Neural Network-Based Flight Control Technology for New Aerospace Systems Designs with a Pilot in the Loop. Gen II objectives include; a) Implement and Fly a Direct Adaptive Neural Network Based Flight Controller; b) Demonstrate the Ability of the System to Adapt to Simulated System Failures: 1) Suppress Transients Associated with Failure; 2) Re-Establish Sufficient Control and Handling of Vehicle for Safe Recovery. c) Provide Flight Experience for Development of Verification and Validation Processes for Flight Critical Neural Network Software.

  1. Adaptive Augmenting Control Flight Characterization Experiment on an F/A-18

    NASA Technical Reports Server (NTRS)

    VanZwieten, Tannen S.; Gilligan, Eric T.; Wall, John H.; Orr, Jeb S.; Miller, Christopher J.; Hanson, Curtis E.

    2014-01-01

    The NASA Marshall Space Flight Center (MSFC) Flight Mechanics and Analysis Division developed an Adaptive Augmenting Control (AAC) algorithm for launch vehicles that improves robustness and performance by adapting an otherwise welltuned classical control algorithm to unexpected environments or variations in vehicle dynamics. This AAC algorithm is currently part of the baseline design for the SLS Flight Control System (FCS), but prior to this series of research flights it was the only component of the autopilot design that had not been flight tested. The Space Launch System (SLS) flight software prototype, including the adaptive component, was recently tested on a piloted aircraft at Dryden Flight Research Center (DFRC) which has the capability to achieve a high level of dynamic similarity to a launch vehicle. Scenarios for the flight test campaign were designed specifically to evaluate the AAC algorithm to ensure that it is able to achieve the expected performance improvements with no adverse impacts in nominal or nearnominal scenarios. Having completed the recent series of flight characterization experiments on DFRC's F/A-18, the AAC algorithm's capability, robustness, and reproducibility, have been successfully demonstrated. Thus, the entire SLS control architecture has been successfully flight tested in a relevant environment. This has increased NASA's confidence that the autopilot design is ready to fly on the SLS Block I vehicle and will exceed the performance of previous architectures.

  2. Adaptive aerostructures: the first decade of flight on uninhabited aerial vehicles

    NASA Astrophysics Data System (ADS)

    Barrett, Ronald M.

    2004-07-01

    Although many subscale aircraft regularly fly with adaptive materials in sensors and small components in secondary subsystems, only a handful have flown with adaptive aerostructures as flight critical, enabling components. This paper reviews several families of adaptive aerostructures which have enabled or significantly enhanced flightworthy uninhabited aerial vehicles (UAVs), including rotary and fixed wing aircraft, missiles and munitions. More than 40 adaptive aerostructures programs which have had a direct connection to flight test and/or production UAVs, ranging from hover through hypersonic, sea-level to exo-stratospheric are examined. Adaptive material type, design Mach range, test methods, aircraft configuration and performance of each of the designs are presented. An historical analysis shows the evolution of flightworthy adaptive aerostructures from the earliest staggering flights in 1994 to modern adaptive UAVs supporting live-fire exercises in harsh military environments. Because there are profound differences between bench test, wind tunnel test, flight test and military grade flightworthy adaptive aerostructures, some of the most mature industrial design and fabrication techniques in use today will be outlined. The paper concludes with an example of the useful load and performance expansions which are seen on an industrial, military-grade UAV through the use of properly designed, flight-hardened adaptive aerostructures.

  3. Simulation Based Evaluation of Integrated Adaptive Control and Flight Planning Technologies

    NASA Technical Reports Server (NTRS)

    Campbell, Stefan Forrest; Kaneshige, John T.

    2008-01-01

    The objective of this work is to leverage NASA resources to enable effective evaluation of resilient aircraft technologies through simulation. This includes examining strengths and weaknesses of adaptive controllers, emergency flight planning algorithms, and flight envelope determination algorithms both individually and as an integrated package.

  4. Flight Test of Composite Model Reference Adaptive Control (CMRAC) Augmentation Using NASA AirSTAR Infrastructure

    NASA Technical Reports Server (NTRS)

    Gregory, Irene M.; Gadient, ROss; Lavretsky, Eugene

    2011-01-01

    This paper presents flight test results of a robust linear baseline controller with and without composite adaptive control augmentation. The flight testing was conducted using the NASA Generic Transport Model as part of the Airborne Subscale Transport Aircraft Research system at NASA Langley Research Center.

  5. Internal Arrangement of the Multiple Docking Adapter Illustration

    NASA Technical Reports Server (NTRS)

    1970-01-01

    This cutaway drawing details the internal design of the Skylab Multiple Docking Adapter (MDA). The MDA, built under the direction of the Marshall Space Flight Center, housed various Skylab control and experiment units, and provided a docking port for the Apollo Command Module (CM).

  6. Aeroelastic Deformation: Adaptation of Wind Tunnel Measurement Concepts to Full-Scale Vehicle Flight Testing

    NASA Technical Reports Server (NTRS)

    Burner, Alpheus W.; Lokos, William A.; Barrows, Danny A.

    2005-01-01

    The adaptation of a proven wind tunnel test technique, known as Videogrammetry, to flight testing of full-scale vehicles is presented. A description is presented of the technique used at NASA's Dryden Flight Research Center for the measurement of the change in wing twist and deflection of an F/A-18 research aircraft as a function of both time and aerodynamic load. Requirements for in-flight measurements are compared and contrasted with those for wind tunnel testing. The methodology for the flight-testing technique and differences compared to wind tunnel testing are given. Measurement and operational comparisons to an older in-flight system known as the Flight Deflection Measurement System (FDMS) are presented.

  7. Physiological aeroecology: Anatomical and physiological adaptations for flight

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Flight has evolved independently in birds, bats, and insects and was present in the Mesozoic pterosaurians that have disappeared. Of the roughly 1 million living animal species, more than three-quarters are flying insects. Flying is an extremely successful way of locomotion. At first glance this see...

  8. [The adaptive-compensatory reactions in adjusting to space flights].

    PubMed

    Volozhin, A I

    1995-01-01

    In this paper an evolutionary approach is used to substantiate the steps of life evolution on Earth towards overcoming the gravitational forces with the formation of metabolic cycles controlling the energetic of anti-G processes. The step of an adaptation to hypogravity was similar to return of surface animals to an aquatic environment. The impossibility of coming back to land was the "price" of adaptation to the aquatic environment. This phenomenon was used by the author as a logical model of human adaptation to a weightless environment. The concept of adaptation is examined from two points of view: adaptation and compensation. The scheme contains 4 steps of adaptation to space mission environments: Step I-preadaptation (phase of primary reactions); Step II-compensation of body structures not being in line with the conditions of a novel environment; Step III-the formation of parameters of an organism corresponding to the norm of adaptation to weightlessness; Step IV-the return of cosmonauts to Earth under hypergravity conditions. In compliance with these steps we consider the tasks of supporting space missions to facilitate body readaptation after return to Earth, i.e., to decrease the "price" of adaptation.

  9. Implementation of an Adaptive Controller System from Concept to Flight Test

    NASA Technical Reports Server (NTRS)

    Larson, Richard R.; Burken, John J.; Butler, Bradley S.; Yokum, Steve

    2009-01-01

    The National Aeronautics and Space Administration Dryden Flight Research Center (Edwards, California) is conducting ongoing flight research using adaptive controller algorithms. A highly modified McDonnell-Douglas NF-15B airplane called the F-15 Intelligent Flight Control System (IFCS) is used to test and develop these algorithms. Modifications to this airplane include adding canards and changing the flight control systems to interface a single-string research controller processor for neural network algorithms. Research goals include demonstration of revolutionary control approaches that can efficiently optimize aircraft performance in both normal and failure conditions and advancement of neural-network-based flight control technology for new aerospace system designs. This report presents an overview of the processes utilized to develop adaptive controller algorithms during a flight-test program, including a description of initial adaptive controller concepts and a discussion of modeling formulation and performance testing. Design finalization led to integration with the system interfaces, verification of the software, validation of the hardware to the requirements, design of failure detection, development of safety limiters to minimize the effect of erroneous neural network commands, and creation of flight test control room displays to maximize human situational awareness; these are also discussed.

  10. Towards Validation of an Adaptive Flight Control Simulation Using Statistical Emulation

    NASA Technical Reports Server (NTRS)

    He, Yuning; Lee, Herbert K. H.; Davies, Misty D.

    2012-01-01

    Traditional validation of flight control systems is based primarily upon empirical testing. Empirical testing is sufficient for simple systems in which a.) the behavior is approximately linear and b.) humans are in-the-loop and responsible for off-nominal flight regimes. A different possible concept of operation is to use adaptive flight control systems with online learning neural networks (OLNNs) in combination with a human pilot for off-nominal flight behavior (such as when a plane has been damaged). Validating these systems is difficult because the controller is changing during the flight in a nonlinear way, and because the pilot and the control system have the potential to co-adapt in adverse ways traditional empirical methods are unlikely to provide any guarantees in this case. Additionally, the time it takes to find unsafe regions within the flight envelope using empirical testing means that the time between adaptive controller design iterations is large. This paper describes a new concept for validating adaptive control systems using methods based on Bayesian statistics. This validation framework allows the analyst to build nonlinear models with modal behavior, and to have an uncertainty estimate for the difference between the behaviors of the model and system under test.

  11. An Expert System Framework for Adaptive Evidential Reasoning: Application to In-Flight Route Re-Planning

    DTIC Science & Technology

    1986-03-21

    DECISION SCIENCE CON5ORKIUM, INK. YE AN EXPERT SYSTEM FRANIEWORK FOR ADAPTIVE EVIDENTIAL REASONING: APPLICATION T O IN-FLIGHT ROUTE RE-PLANNING...00-00-1986 to 00-00-1986 4. TITLE AND SUBTITLE An Expert System Framework for Adaptive Evidential Reasoning: Application to In-Flight Route Re...EXPERT SYSTEM FRAMEWORK FOR ADAPTIVE EVIDENTIAL REASONING: APPLICATION T O IN-FLIGHT ROUTE RE-PLANNING Marvin S. Cohen, Kathryn B. Laskey, James

  12. LaPlace Transform1 Adaptive Control Law in Support of Large Flight Envelope Modeling Work

    NASA Technical Reports Server (NTRS)

    Gregory, Irene M.; Xargay, Enric; Cao, Chengyu; Hovakimyan, Naira

    2011-01-01

    This paper presents results of a flight test of the L1 adaptive control architecture designed to directly compensate for significant uncertain cross-coupling in nonlinear systems. The flight test was conducted on the subscale turbine powered Generic Transport Model that is an integral part of the Airborne Subscale Transport Aircraft Research system at the NASA Langley Research Center. The results presented are in support of nonlinear aerodynamic modeling and instrumentation calibration.

  13. Highly integrated digital electronic control: Digital flight control, aircraft model identification, and adaptive engine control

    NASA Technical Reports Server (NTRS)

    Baer-Riedhart, Jennifer L.; Landy, Robert J.

    1987-01-01

    The highly integrated digital electronic control (HIDEC) program at NASA Ames Research Center, Dryden Flight Research Facility is a multiphase flight research program to quantify the benefits of promising integrated control systems. McDonnell Aircraft Company is the prime contractor, with United Technologies Pratt and Whitney Aircraft, and Lear Siegler Incorporated as major subcontractors. The NASA F-15A testbed aircraft was modified by the HIDEC program by installing a digital electronic flight control system (DEFCS) and replacing the standard F100 (Arab 3) engines with F100 engine model derivative (EMD) engines equipped with digital electronic engine controls (DEEC), and integrating the DEEC's and DEFCS. The modified aircraft provides the capability for testing many integrated control modes involving the flight controls, engine controls, and inlet controls. This paper focuses on the first two phases of the HIDEC program, which are the digital flight control system/aircraft model identification (DEFCS/AMI) phase and the adaptive engine control system (ADECS) phase.

  14. Approach for Structurally Clearing an Adaptive Compliant Trailing Edge Flap for Flight

    NASA Technical Reports Server (NTRS)

    Miller, Eric J.; Lokos, William A.; Cruz, Josue; Crampton, Glen; Stephens, Craig A.; Kota, Sridhar; Ervin, Gregory; Flick, Pete

    2015-01-01

    The Adaptive Compliant Trailing Edge (ACTE) flap was flown on the National Aeronautics and Space Administration (NASA) Gulfstream GIII testbed at the NASA Armstrong Flight Research Center. This smoothly curving flap replaced the existing Fowler flaps creating a seamless control surface. This compliant structure, developed by FlexSys Inc. in partnership with the Air Force Research Laboratory, supported NASA objectives for airframe structural noise reduction, aerodynamic efficiency, and wing weight reduction through gust load alleviation. A thorough structures airworthiness approach was developed to move this project safely to flight. A combination of industry and NASA standard practice require various structural analyses, ground testing, and health monitoring techniques for showing an airworthy structure. This paper provides an overview of compliant structures design, the structural ground testing leading up to flight, and the flight envelope expansion and monitoring strategy. Flight data will be presented, and lessons learned along the way will be highlighted.

  15. Implementation of an Adaptive Controller System from Concept to Flight Test

    NASA Technical Reports Server (NTRS)

    Larson, Richard R.; Burken, John J.; Butler, Bradley S.

    2009-01-01

    The National Aeronautics and Space Administration Dryden Flight Research Center (Edwards, California) is conducting ongoing flight research using adaptive controller algorithms. A highly modified McDonnell-Douglas NF-15B airplane called the F-15 Intelligent Flight Control System (IFCS) was used for these algorithms. This airplane has been modified by the addition of canards and by changing the flight control systems to interface a single-string research controller processor for neural network algorithms. Research goals included demonstration of revolutionary control approaches that can efficiently optimize aircraft performance for both normal and failure conditions, and to advance neural-network-based flight control technology for new aerospace systems designs. Before the NF-15B IFCS airplane was certified for flight test, however, certain processes needed to be completed. This paper presents an overview of these processes, including a description of the initial adaptive controller concepts followed by a discussion of modeling formulation and performance testing. Upon design finalization, the next steps are: integration with the system interfaces, verification of the software, validation of the hardware to the requirements, design of failure detection, development of safety limiters to minimize the effect of erroneous neural network commands, and creation of flight test control room displays to maximize human situational awareness.

  16. Adaptive support vector regression for UAV flight control.

    PubMed

    Shin, Jongho; Jin Kim, H; Kim, Youdan

    2011-01-01

    This paper explores an application of support vector regression for adaptive control of an unmanned aerial vehicle (UAV). Unlike neural networks, support vector regression (SVR) generates global solutions, because SVR basically solves quadratic programming (QP) problems. With this advantage, the input-output feedback-linearized inverse dynamic model and the compensation term for the inversion error are identified off-line, which we call I-SVR (inversion SVR) and C-SVR (compensation SVR), respectively. In order to compensate for the inversion error and the unexpected uncertainty, an online adaptation algorithm for the C-SVR is proposed. Then, the stability of the overall error dynamics is analyzed by the uniformly ultimately bounded property in the nonlinear system theory. In order to validate the effectiveness of the proposed adaptive controller, numerical simulations are performed on the UAV model.

  17. A Decentralized Adaptive Approach to Fault Tolerant Flight Control

    NASA Technical Reports Server (NTRS)

    Wu, N. Eva; Nikulin, Vladimir; Heimes, Felix; Shormin, Victor

    2000-01-01

    This paper briefly reports some results of our study on the application of a decentralized adaptive control approach to a 6 DOF nonlinear aircraft model. The simulation results showed the potential of using this approach to achieve fault tolerant control. Based on this observation and some analysis, the paper proposes a multiple channel adaptive control scheme that makes use of the functionally redundant actuating and sensing capabilities in the model, and explains how to implement the scheme to tolerate actuator and sensor failures. The conditions, under which the scheme is applicable, are stated in the paper.

  18. Energetic Metabolism and Biochemical Adaptation: A Bird Flight Muscle Model

    ERIC Educational Resources Information Center

    Rioux, Pierre; Blier, Pierre U.

    2006-01-01

    The main objective of this class experiment is to measure the activity of two metabolic enzymes in crude extract from bird pectoral muscle and to relate the differences to their mode of locomotion and ecology. The laboratory is adapted to stimulate the interest of wildlife management students to biochemistry. The enzymatic activities of cytochrome…

  19. Multiple Model Parameter Adaptive Control for In-Flight Simulation.

    DTIC Science & Technology

    1988-03-01

    dynamics of an aircraft. The plant is control- lable by a proportional-plus-integral ( PI ) control law. This section describes two methods of calculating...adaptive model-following PI control law [20-24]. The control law bases its control gains upon the parameters of a linear difference equation model which

  20. Integration of Online Parameter Identification and Neural Network for In-Flight Adaptive Control

    NASA Technical Reports Server (NTRS)

    Hageman, Jacob J.; Smith, Mark S.; Stachowiak, Susan

    2003-01-01

    An indirect adaptive system has been constructed for robust control of an aircraft with uncertain aerodynamic characteristics. This system consists of a multilayer perceptron pre-trained neural network, online stability and control derivative identification, a dynamic cell structure online learning neural network, and a model following control system based on the stochastic optimal feedforward and feedback technique. The pre-trained neural network and model following control system have been flight-tested, but the online parameter identification and online learning neural network are new additions used for in-flight adaptation of the control system model. A description of the modification and integration of these two stand-alone software packages into the complete system in preparation for initial flight tests is presented. Open-loop results using both simulation and flight data, as well as closed-loop performance of the complete system in a nonlinear, six-degree-of-freedom, flight validated simulation, are analyzed. Results show that this online learning system, in contrast to the nonlearning system, has the ability to adapt to changes in aerodynamic characteristics in a real-time, closed-loop, piloted simulation, resulting in improved flying qualities.

  1. [Adaptation of water-electrolytes metabolism to space flight and in its imitation].

    PubMed

    Noskov, V B

    2013-01-01

    50-years study of water-electrolytes exchange, the condition of water environments of the organism and the hormonal regulation in space flights, and also in postflight period or in its on ground modeling (hypokinesia, bed rest, immersion etc.) has shown the important role of the water-salt homeostasis in adaptation of the human and animal organisms to weightlessness. It has been revealed, that in weightlessness conditions for development of negative balance of a liquid (hypohydration) and the basic electrolytes are created. After the termination of long space flights attributes of development adaptive reactions compensating for extracellular liquid's volume come to light. In order to assess the state of the kidneys and water-electrolyte metabolism in cosmonauts and investigators, functional load tests and especial methods of diagnostic were developed. This is the basis for researches directed on improvement of the scheme of correction hydrogenous the status of an organism of the cosmonauts at the different stages of flight.

  2. Design and Flight Tests of an Adaptive Control System Employing Normal-Acceleration Command

    NASA Technical Reports Server (NTRS)

    McNeill, Water E.; McLean, John D.; Hegarty, Daniel M.; Heinle, Donovan R.

    1961-01-01

    An adaptive control system employing normal-acceleration command has been designed with the aid of an analog computer and has been flight tested. The design of the system was based on the concept of using a mathematical model in combination with a high gain and a limiter. The study was undertaken to investigate the application of a system of this type to the task of maintaining nearly constant dynamic longitudinal response of a piloted airplane over the flight envelope without relying on air data measurements for gain adjustment. The range of flight conditions investigated was between Mach numbers of 0.36 and 1.15 and altitudes of 10,000 and 40,000 feet. The final adaptive system configuration was derived from analog computer tests, in which the physical airplane control system and much of the control circuitry were included in the loop. The method employed to generate the feedback signals resulted in a model whose characteristics varied somewhat with changes in flight condition. Flight results showed that the system limited the variation in longitudinal natural frequency of the adaptive airplane to about half that of the basic airplane and that, for the subsonic cases, the damping ratio was maintained between 0.56 and 0.69. The system also automatically compensated for the transonic trim change. Objectionable features of the system were an exaggerated sensitivity of pitch attitude to gust disturbances, abnormally large pitch attitude response for a given pilot input at low speeds, and an initial delay in normal-acceleration response to pilot control at all flight conditions. The adaptive system chatter of +/-0.05 to +/-0.10 of elevon at about 9 cycles per second (resulting in a maximum airplane normal-acceleration response of from +/-0.025 g to +/- 0.035 g) was considered by the pilots to be mildly objectionable but tolerable.

  3. [Intensity of lipid peroxidation and antioxidant protection system indices during re-adaptation period after long-term space flights at the international space station].

    PubMed

    Zhuravleva, O A; Morukov, B V; Markin, A A; Vostrikova, L V; Zabolotskaia, I V

    2011-01-01

    In the blood serum of seventeen members of crews which participated in 14 orbital expeditions to the International Space Station with the duration of 125 to 217 days, during the pre-flight period and on the day of landing on the 1st, 7th and 14th days of the rehabilitation period (RP) the content of LPO products was determined, namely diene conjugates (DC), malon dialdehyde (MDA), shiffbases (SB) and the main lipid oxidant - tocopherol (TP). The group of astronauts who made landing in the Space Shuttle spacecraft (8 persons) and the group of astronauts who accomplished space mission in the Soyus TM spacecraft (9 persons) demonstrated a decrease in DC and MDA levels with a rise in TF concentration in the course of the rehabilitation period. Changes in the group of the American spacecraft astronauts were more pronounced. LPO inhibition during the rehabilitation period is recognized [treated] as an adequate reaction to the stress caused by re-adaptation to the ground conditions. Also are discussed probable mechanisms of intergroup differencies in LPO intensity degree: biomembrane phase state changing under the influence of overloads during de-orbiting and stress response intensity during landing in different types of spacecraft.

  4. Flight Test Results from the NF-15B Intelligent Flight Control System (IFCS) Project with Adaptation to a Simulated Stabilator Failure

    NASA Technical Reports Server (NTRS)

    Bosworth, John T.; Williams-Hayes, Peggy S.

    2007-01-01

    Adaptive flight control systems have the potential to be more resilient to extreme changes in airplane behavior. Extreme changes could be a result of a system failure or of damage to the airplane. A direct adaptive neural-network-based flight control system was developed for the National Aeronautics and Space Administration NF-15B Intelligent Flight Control System airplane and subjected to an inflight simulation of a failed (frozen) (unmovable) stabilator. Formation flight handling qualities evaluations were performed with and without neural network adaptation. The results of these flight tests are presented. Comparison with simulation predictions and analysis of the performance of the adaptation system are discussed. The performance of the adaptation system is assessed in terms of its ability to decouple the roll and pitch response and reestablish good onboard model tracking. Flight evaluation with the simulated stabilator failure and adaptation engaged showed that there was generally improvement in the pitch response; however, a tendency for roll pilot-induced oscillation was experienced. A detailed discussion of the cause of the mixed results is presented.

  5. Flight Test Results from the NF-15B Intelligent Flight Control System (IFCS) Project with Adaptation to a Simulated Stabilator Failure

    NASA Technical Reports Server (NTRS)

    Bosworth, John T.; Williams-Hayes, Peggy S.

    2010-01-01

    Adaptive flight control systems have the potential to be more resilient to extreme changes in airplane behavior. Extreme changes could be a result of a system failure or of damage to the airplane. A direct adaptive neural-network-based flight control system was developed for the National Aeronautics and Space Administration NF-15B Intelligent Flight Control System airplane and subjected to an inflight simulation of a failed (frozen) (unmovable) stabilator. Formation flight handling qualities evaluations were performed with and without neural network adaptation. The results of these flight tests are presented. Comparison with simulation predictions and analysis of the performance of the adaptation system are discussed. The performance of the adaptation system is assessed in terms of its ability to decouple the roll and pitch response and reestablish good onboard model tracking. Flight evaluation with the simulated stabilator failure and adaptation engaged showed that there was generally improvement in the pitch response; however, a tendency for roll pilot-induced oscillation was experienced. A detailed discussion of the cause of the mixed results is presented.

  6. Approach for Structurally Clearing an Adaptive Compliant Trailing Edge Flap for Flight

    NASA Technical Reports Server (NTRS)

    Miller, Eric J.; Lokos, William A.; Cruz, Josue; Crampton, Glen; Stephens, Craig A.; Kota, Sridhar; Ervin, Gregory; Flick, Pete

    2015-01-01

    The Adaptive Compliant Trailing Edge (ACTE) flap was flown on the NASA Gulfstream GIII test bed at the NASA Armstrong Flight Research Center. This smoothly curving flap replaced the existing Fowler flaps creating a seamless control surface. This compliant structure, developed by FlexSys Inc. in partnership with Air Force Research Laboratory, supported NASA objectives for airframe structural noise reduction, aerodynamic efficiency, and wing weight reduction through gust load alleviation. A thorough structures airworthiness approach was developed to move this project safely to flight.

  7. Launch Vehicle Manual Steering with Adaptive Augmenting Control In-flight Evaluations of Adverse Interactions Using a Piloted Aircraft

    NASA Technical Reports Server (NTRS)

    Hanson, Curt; Miller, Chris; Wall, John H.; Vanzwieten, Tannen S.; Gilligan, Eric; Orr, Jeb S.

    2015-01-01

    An adaptive augmenting control algorithm for the Space Launch System has been developed at the Marshall Space Flight Center as part of the launch vehicles baseline flight control system. A prototype version of the SLS flight control software was hosted on a piloted aircraft at the Armstrong Flight Research Center to demonstrate the adaptive controller on a full-scale realistic application in a relevant flight environment. Concerns regarding adverse interactions between the adaptive controller and a proposed manual steering mode were investigated by giving the pilot trajectory deviation cues and pitch rate command authority. Two NASA research pilots flew a total of twenty five constant pitch-rate trajectories using a prototype manual steering mode with and without adaptive control.

  8. Hybrid Decompositional Verification for Discovering Failures in Adaptive Flight Control Systems

    NASA Technical Reports Server (NTRS)

    Thompson, Sarah; Davies, Misty D.; Gundy-Burlet, Karen

    2010-01-01

    Adaptive flight control systems hold tremendous promise for maintaining the safety of a damaged aircraft and its passengers. However, most currently proposed adaptive control methodologies rely on online learning neural networks (OLNNs), which necessarily have the property that the controller is changing during the flight. These changes tend to be highly nonlinear, and difficult or impossible to analyze using standard techniques. In this paper, we approach the problem with a variant of compositional verification. The overall system is broken into components. Undesirable behavior is fed backwards through the system. Components which can be solved using formal methods techniques explicitly for the ranges of safe and unsafe input bounds are treated as white box components. The remaining black box components are analyzed with heuristic techniques that try to predict a range of component inputs that may lead to unsafe behavior. The composition of these component inputs throughout the system leads to overall system test vectors that may elucidate the undesirable behavior

  9. Human physiological adaptation to extended Space Flight and its implications for Space Station

    NASA Technical Reports Server (NTRS)

    Kutyna, F. A.; Shumate, W. H.

    1985-01-01

    Current work evaluating short-term space flight physiological data on the homeostatic changes due to weightlessness is presented as a means of anticipating Space Station long-term effects. An integrated systems analysis of current data shows a vestibulo-sensory adaptation within days; a loss of body mass, fluids, and electrolytes, stabilizing in a month; and a loss in red cell mass over a month. But bone demineralization which did not level off is seen as the biggest concern. Computer algorithms have been developed to simulate the human adaptation to weightlessness. So far these paradigms have been backed up by flight data and it is hoped that they will provide valuable information for future Space Station design. A series of explanatory schematics is attached.

  10. Adaptation of neuromuscular activation patterns during treadmill walking after long-duration space flight

    NASA Astrophysics Data System (ADS)

    Layne, C. S.; Lange, G. W.; Pruett, C. J.; McDonald, P. V.; Merkle, L. A.; Mulavara, A. P.; Smith, S. L.; Kozlovskaya, I. B.; Bloomberg, J. J.

    The precise neuromuscular control needed for optimal locomotion, particularly around heel strike and toe off, is known to be compromised after short duration (8- to 15-day) space flight. We hypothesized here that longer exposure to weightlessness would result in maladaptive neuromuscular activation during postflight treadmill walking. We also hypothesized that space flight would affect the ability of the sensory-motor control system to generate adaptive neuromuscular activation patterns in response to changes in visual target distance during postflight treadmill walking. Seven crewmembers, who completed 3- to 6-month missions, walked on a motorized treadmill while visually fixating on a target placed 30 cm (NEAR) or 2 m (FAR) from the subject's eyes. Electronic foot switch data and surface electromyography were collected from selected muscles of the right lower limb. Results indicate that the phasic features of neuromuscular activation were moderately affected and the relative amplitude of activity in the tibialis anterior and rectus femoris around toe off changed after space flight. Changes also were evident after space flight in how these muscles adapted to the shift in visual target distance.

  11. Intelligent adaptive nonlinear flight control for a high performance aircraft with neural networks.

    PubMed

    Savran, Aydogan; Tasaltin, Ramazan; Becerikli, Yasar

    2006-04-01

    This paper describes the development of a neural network (NN) based adaptive flight control system for a high performance aircraft. The main contribution of this work is that the proposed control system is able to compensate the system uncertainties, adapt to the changes in flight conditions, and accommodate the system failures. The underlying study can be considered in two phases. The objective of the first phase is to model the dynamic behavior of a nonlinear F-16 model using NNs. Therefore a NN-based adaptive identification model is developed for three angular rates of the aircraft. An on-line training procedure is developed to adapt the changes in the system dynamics and improve the identification accuracy. In this procedure, a first-in first-out stack is used to store a certain history of the input-output data. The training is performed over the whole data in the stack at every stage. To speed up the convergence rate and enhance the accuracy for achieving the on-line learning, the Levenberg-Marquardt optimization method with a trust region approach is adapted to train the NNs. The objective of the second phase is to develop intelligent flight controllers. A NN-based adaptive PID control scheme that is composed of an emulator NN, an estimator NN, and a discrete time PID controller is developed. The emulator NN is used to calculate the system Jacobian required to train the estimator NN. The estimator NN, which is trained on-line by propagating the output error through the emulator, is used to adjust the PID gains. The NN-based adaptive PID control system is applied to control three angular rates of the nonlinear F-16 model. The body-axis pitch, roll, and yaw rates are fed back via the PID controllers to the elevator, aileron, and rudder actuators, respectively. The resulting control system has learning, adaptation, and fault-tolerant abilities. It avoids the storage and interpolation requirements for the too many controller parameters of a typical flight control

  12. Adaptive estimation and control with application to vision-based autonomous formation flight

    NASA Astrophysics Data System (ADS)

    Sattigeri, Ramachandra

    2007-05-01

    Modern Unmanned Aerial Vehicles (UAVs) are equipped with vision sensors because of their light-weight, low-cost characteristics and also their ability to provide a rich variety of information of the environment in which the UAVs are navigating in. The problem of vision based autonomous flight is very difficult and challenging since it requires bringing together concepts from image processing and computer vision, target tracking and state estimation, and flight guidance and control. This thesis focuses on the adaptive state estimation, guidance and control problems involved in vision-based formation flight. Specifically, the thesis presents a composite adaptation approach to the partial state estimation of a class of nonlinear systems with unmodeled dynamics. In this approach, a linear time-varying Kalman filter is the nominal state estimator which is augmented by the output of an adaptive neural network (NN) that is trained with two error signals. The benefit of the proposed approach is in its faster and more accurate adaptation to the modeling errors over a conventional approach. The thesis also presents two approaches to the design of adaptive guidance and control (G&C) laws for line-of-sight formation flight. In the first approach, the guidance and autopilot systems are designed separately and then combined together by assuming time-scale separation. The second approach is based on integrating the guidance and autopilot design process. The developed G&C laws using both approaches are adaptive to unmodeled leader aircraft acceleration and to own aircraft aerodynamic uncertainties. The thesis also presents theoretical justification based on Lyapunov-like stability analysis for integrating the adaptive state estimation and adaptive G&C designs. All the developed designs are validated in nonlinear, 6DOF fixed-wing aircraft simulations. Finally, the thesis presents a decentralized coordination strategy for vision-based multiple-aircraft formation control. In this

  13. [Mechanisms of natural variability at adaptation of human physiological systems to conditions of space flight].

    PubMed

    Larina, I M; Nosovskiĭ, A M; Grigor'ev, A I

    2012-01-01

    This article analyzes the physiological data using the principle of invariant relationships, to reveal the mechanisms of adaptive variability. It was used physical-chemical, biochemical, and hormonal blood parameters of cosmonauts who have committed short-term and long space flights. These results suggest that application of the methods of fractal geometry to quantitative estimates of homeostasis allows to allocate the processes depending on the increase/decrease of adaptive variability and fix the state of stability or instability of certain physiological regulatory subsystems, due to mobility and to reduce the level of stability which remains stable internal structure of relationships throughout the body.

  14. Peculiarities of transformation of adaptation level of the astronaut in conditions of long-lasting flight

    NASA Astrophysics Data System (ADS)

    Padashulya, H.; Prisnyakova, L.; Prisnyakov, V.

    Prognostication of the development of adverse factors of psychological processes in the personality of the astronaut who time and again feels transformation of internal structure of his personality is one of cardinal problems of the long-lasting flight Adaptation to changing conditions of long-lasting flight is of particular importance because it has an effect on the efficiency of discharged functions and mutual relations in the team The fact of standard psychological changes emerging in the personality being in the state of structural transformations is the precondition for the possibility of prognostication Age-specific gender and temperamental differences in the personality enable to standardize these changes Examination of the process of transformation of adaptation level of the personality in the varied environment depending on the type of temperament and constituents age and gender is chief object of the report In the report it is shown that in the process of transformation of adaptation parameters - attitude to guillemotleft work guillemotright guillemotleft family guillemotright guillemotleft environment guillemotright and guillemotleft ego guillemotright - the changes can go in two directions - in the direction of increase and decline of indexes The trend of increase enables to accumulate them and form potentiality to reduce or increase the level of personality adaptation There is a hypothesis that the dynamics of the process of transformation of adaptation parameter is shown up in the orientation of increase of

  15. Case Study: Test Results of a Tool and Method for In-Flight, Adaptive Control System Verification on a NASA F-15 Flight Research Aircraft

    NASA Technical Reports Server (NTRS)

    Jacklin, Stephen A.; Schumann, Johann; Guenther, Kurt; Bosworth, John

    2006-01-01

    Adaptive control technologies that incorporate learning algorithms have been proposed to enable autonomous flight control and to maintain vehicle performance in the face of unknown, changing, or poorly defined operating environments [1-2]. At the present time, however, it is unknown how adaptive algorithms can be routinely verified, validated, and certified for use in safety-critical applications. Rigorous methods for adaptive software verification end validation must be developed to ensure that. the control software functions as required and is highly safe and reliable. A large gap appears to exist between the point at which control system designers feel the verification process is complete, and when FAA certification officials agree it is complete. Certification of adaptive flight control software verification is complicated by the use of learning algorithms (e.g., neural networks) and degrees of system non-determinism. Of course, analytical efforts must be made in the verification process to place guarantees on learning algorithm stability, rate of convergence, and convergence accuracy. However, to satisfy FAA certification requirements, it must be demonstrated that the adaptive flight control system is also able to fail and still allow the aircraft to be flown safely or to land, while at the same time providing a means of crew notification of the (impending) failure. It was for this purpose that the NASA Ames Confidence Tool was developed [3]. This paper presents the Confidence Tool as a means of providing in-flight software assurance monitoring of an adaptive flight control system. The paper will present the data obtained from flight testing the tool on a specially modified F-15 aircraft designed to simulate loss of flight control faces.

  16. In-flight adaptive performance optimization (APO) control using redundant control effectors of an aircraft

    NASA Technical Reports Server (NTRS)

    Gilyard, Glenn B. (Inventor)

    1999-01-01

    Practical application of real-time (or near real-time) Adaptive Performance Optimization (APO) is provided for a transport aircraft in steady climb, cruise, turn descent or other flight conditions based on measurements and calculations of incremental drag from a forced response maneuver of one or more redundant control effectors defined as those in excess of the minimum set of control effectors required to maintain the steady flight condition in progress. The method comprises the steps of applying excitation in a raised-cosine form over an interval of from 100 to 500 sec. at the rate of 1 to 10 sets/sec of excitation, and data for analysis is gathered in sets of measurements made during the excitation to calculate lift and drag coefficients C.sub.L and C.sub.D from two equations, one for each coefficient. A third equation is an expansion of C.sub.D as a function of parasitic drag, induced drag, Mach and altitude drag effects, and control effector drag, and assumes a quadratic variation of drag with positions .delta..sub.i of redundant control effectors i=1 to n. The third equation is then solved for .delta..sub.iopt the optimal position of redundant control effector i, which is then used to set the control effector i for optimum performance during the remainder of said steady flight or until monitored flight conditions change by some predetermined amount as determined automatically or a predetermined minimum flight time has elapsed.

  17. Design Process of Flight Vehicle Structures for a Common Bulkhead and an MPCV Spacecraft Adapter

    NASA Technical Reports Server (NTRS)

    Aggarwal, Pravin; Hull, Patrick V.

    2015-01-01

    Design and manufacturing space flight vehicle structures is a skillset that has grown considerably at NASA during that last several years. Beginning with the Ares program and followed by the Space Launch System (SLS); in-house designs were produced for both the Upper Stage and the SLS Multipurpose crew vehicle (MPCV) spacecraft adapter. Specifically, critical design review (CDR) level analysis and flight production drawing were produced for the above mentioned hardware. In particular, the experience of this in-house design work led to increased manufacturing infrastructure for both Marshal Space Flight Center (MSFC) and Michoud Assembly Facility (MAF), improved skillsets in both analysis and design, and hands on experience in building and testing (MSA) full scale hardware. The hardware design and development processes from initiation to CDR and finally flight; resulted in many challenges and experiences that produced valuable lessons. This paper builds on these experiences of NASA in recent years on designing and fabricating flight hardware and examines the design/development processes used, as well as the challenges and lessons learned, i.e. from the initial design, loads estimation and mass constraints to structural optimization/affordability to release of production drawing to hardware manufacturing. While there are many documented design processes which a design engineer can follow, these unique experiences can offer insight into designing hardware in current program environments and present solutions to many of the challenges experienced by the engineering team.

  18. Flight test results from a supercritical mission adaptive wing with smooth variable camber

    NASA Technical Reports Server (NTRS)

    Powers, Sheryll Goecke; Webb, Lannie D.; Friend, Edward L.; Lokos, William A.

    1992-01-01

    The mission adaptive wing (MAW) consisted of leading- and trailing-edge variable-camber surfaces that could be deflected in flight to provide a near-ideal wing camber shape for any flight condition. These surfaces featured smooth, flexible upper surfaces and fully enclosed lower surfaces, distinguishing them from conventional flaps that have discontinuous surfaces and exposed or semiexposed mechanisms. Camber shape was controlled by either a manual or automatic flight control system. The wing and aircraft were extensively instrumented to evaluate the local flow characteristics and the total aircraft performance. This paper discusses the interrelationships between the wing pressure, buffet, boundary-layer and flight deflection measurement system analyses and describes the flight maneuvers used to obtain the data. The results are for a wing sweep of 26 deg, a Mach number of 0.85, leading and trailing-edge cambers (delta(sub LE/TE)) of 0/2 and 5/10, and angles of attack from 3.0 deg to 14.0 deg. For the well-behaved flow of the delta(sub LE/TE) = 0/2 camber, a typical cruise camber shape, the local and global data are in good agreement with respect to the flow properties of the wing. For the delta(sub LE/TE) = 5/10 camber, a maneuvering camber shape, the local and global data have similar trends and conclusions, but not the clear-cut agreement observed for cruise camber.

  19. Flight test results of the fuzzy logic adaptive controller-helicopter (FLAC-H)

    NASA Astrophysics Data System (ADS)

    Wade, Robert L.; Walker, Gregory W.

    1996-05-01

    The fuzzy logic adaptive controller for helicopters (FLAC-H) demonstration is a cooperative effort between the US Army Simulation, Training, and Instrumentation Command (STRICOM), the US Army Aviation and Troop Command, and the US Army Missile Command to demonstrate a low-cost drone control system for both full-scale and sub-scale helicopters. FLAC-H was demonstrated on one of STRICOM's fleet of full-scale rotary-winged target drones. FLAC-H exploits fuzzy logic in its flight control system to provide a robust solution to the control of the helicopter's dynamic, nonlinear system. Straight forward, common sense fuzzy rules governing helicopter flight are processed instead of complex mathematical models. This has resulted in a simplified solution to the complexities of helicopter flight. Incorporation of fuzzy logic reduced the cost of development and should also reduce the cost of maintenance of the system. An adaptive algorithm allows the FLAC-H to 'learn' how to fly the helicopter, enabling the control system to adjust to varying helicopter configurations. The adaptive algorithm, based on genetic algorithms, alters the fuzzy rules and their related sets to improve the performance characteristics of the system. This learning allows FLAC-H to automatically be integrated into a new airframe, reducing the development costs associated with altering a control system for a new or heavily modified aircraft. Successful flight tests of the FLAC-H on a UH-1H target drone were completed in September 1994 at the White Sands Missile Range in New Mexico. This paper discuses the objective of the system, its design, and performance.

  20. Flight Test of an Adaptive Controller and Simulated Failure/Damage on the NASA NF-15B

    NASA Technical Reports Server (NTRS)

    Buschbacher, Mark; Maliska, Heather

    2006-01-01

    The method of flight-testing the Intelligent Flight Control System (IFCS) Second Generation (Gen-2) project on the NASA NF-15B is herein described. The Gen-2 project objective includes flight-testing a dynamic inversion controller augmented by a direct adaptive neural network to demonstrate performance improvements in the presence of simulated failure/damage. The Gen-2 objectives as implemented on the NASA NF-15B created challenges for software design, structural loading limitations, and flight test operations. Simulated failure/damage is introduced by modifying control surface commands, therefore requiring structural loads measurements. Flight-testing began with the validation of a structural loads model. Flight-testing of the Gen-2 controller continued, using test maneuvers designed in a sequenced approach. Success would clear the new controller with respect to dynamic response, simulated failure/damage, and with adaptation on and off. A handling qualities evaluation was conducted on the capability of the Gen-2 controller to restore aircraft response in the presence of a simulated failure/damage. Control room monitoring of loads sensors, flight dynamics, and controller adaptation, in addition to postflight data comparison to the simulation, ensured a safe methodology of buildup testing. Flight-testing continued without major incident to accomplish the project objectives, successfully uncovering strengths and weaknesses of the Gen-2 control approach in flight.

  1. Adaptive Failure Compensation for Aircraft Flight Control Using Engine Differentials: Regulation

    NASA Technical Reports Server (NTRS)

    Yu, Liu; Xidong, Tang; Gang, Tao; Joshi, Suresh M.

    2005-01-01

    The problem of using engine thrust differentials to compensate for rudder and aileron failures in aircraft flight control is addressed in this paper in a new framework. A nonlinear aircraft model that incorporates engine di erentials in the dynamic equations is employed and linearized to describe the aircraft s longitudinal and lateral motion. In this model two engine thrusts of an aircraft can be adjusted independently so as to provide the control flexibility for rudder or aileron failure compensation. A direct adaptive compensation scheme for asymptotic regulation is developed to handle uncertain actuator failures in the linearized system. A design condition is specified to characterize the system redundancy needed for failure compensation. The adaptive regulation control scheme is applied to the linearized model of a large transport aircraft in which the longitudinal and lateral motions are coupled as the result of using engine thrust differentials. Simulation results are presented to demonstrate the effectiveness of the adaptive compensation scheme.

  2. An implementable digital adaptive flight controller designed using stabilized single stage algorithms

    NASA Technical Reports Server (NTRS)

    Kaufman, H.; Alag, G.

    1975-01-01

    Simple mechanical linkages have not solved the many control problems associated with high performance aircraft maneuvering throughout a wide flight envelope. One procedure for retaining uniform handling qualities over such an envelope is to implement a digital adaptive controller. Towards such an implementation an explicit adaptive controller which makes direct use of on-line parameter identification, has been developed and applied to both linearized and nonlinear equations of motion for a typical fighter aircraft. This controller is composed of an on-line weighted least squares parameter identifier, a Kalman state filter, and a model following control law designed using single stage performance indices. Simulation experiments with realistic measurement noise indicate that the proposed adaptive system has the potential for on-board implementation.

  3. Changes in Jump-Down Performance After Space Flight: Short- and Long-Term Adaptation

    NASA Technical Reports Server (NTRS)

    Kofman, I. S.; Reschke, M. F.; Cerisano, J. M.; Fisher, E. A.; Lawrence, E. L.; Peters, B. T.; Bloomberg, J. J.

    2010-01-01

    results demonstrate astronauts adaptive capabilities and full performance recovery within days after flight.

  4. Adaptive Augmenting Control Flight Characterization Experiment on an F/A-18

    NASA Technical Reports Server (NTRS)

    VanZwieten, Tannen S.; Orr, Jeb S.; Wall, John H.; Gilligan, Eric T.

    2014-01-01

    This paper summarizes the Adaptive Augmenting Control (AAC) flight characterization experiments performed using an F/A-18 (TN 853). AAC was designed and developed specifically for launch vehicles, and is currently part of the baseline autopilot design for NASA's Space Launch System (SLS). The scope covered here includes a brief overview of the algorithm (covered in more detail elsewhere), motivation and benefits of flight testing, top-level SLS flight test objectives, applicability of the F/A-18 as a platform for testing a launch vehicle control design, test cases designed to fully vet the AAC algorithm, flight test results, and conclusions regarding the functionality of AAC. The AAC algorithm developed at Marshall Space Flight Center is a forward loop gain multiplicative adaptive algorithm that modifies the total attitude control system gain in response to sensed model errors or undesirable parasitic mode resonances. The AAC algorithm provides the capability to improve or decrease performance by balancing attitude tracking with the mitigation of parasitic dynamics, such as control-structure interaction or servo-actuator limit cycles. In the case of the latter, if unmodeled or mismodeled parasitic dynamics are present that would otherwise result in a closed-loop instability or near instability, the adaptive controller decreases the total loop gain to reduce the interaction between these dynamics and the controller. This is in contrast to traditional adaptive control logic, which focuses on improving performance by increasing gain. The computationally simple AAC attitude control algorithm has stability properties that are reconcilable in the context of classical frequency-domain criteria (i.e., gain and phase margin). The algorithm assumes that the baseline attitude control design is well-tuned for a nominal trajectory and is designed to adapt only when necessary. Furthermore, the adaptation is attracted to the nominal design and adapts only on an as-needed basis

  5. Improved methods in neural network-based adaptive output feedback control, with applications to flight control

    NASA Astrophysics Data System (ADS)

    Kim, Nakwan

    Utilizing the universal approximation property of neural networks, we develop several novel approaches to neural network-based adaptive output feedback control of nonlinear systems, and illustrate these approaches for several flight control applications. In particular, we address the problem of non-affine systems and eliminate the fixed point assumption present in earlier work. All of the stability proofs are carried out in a form that eliminates an algebraic loop in the neural network implementation. An approximate input/output feedback linearizing controller is augmented with a neural network using input/output sequences of the uncertain system. These approaches permit adaptation to both parametric uncertainty and unmodeled dynamics. All physical systems also have control position and rate limits, which may either deteriorate performance or cause instability for a sufficiently high control bandwidth. Here we apply a method for protecting an adaptive process from the effects of input saturation and time delays, known as "pseudo control hedging". This method was originally developed for the state feedback case, and we provide a stability analysis that extends its domain of applicability to the case of output feedback. The approach is illustrated by the design of a pitch-attitude flight control system for a linearized model of an R-50 experimental helicopter, and by the design of a pitch-rate control system for a 58-state model of a flexible aircraft consisting of rigid body dynamics coupled with actuator and flexible modes. A new approach to augmentation of an existing linear controller is introduced. It is especially useful when there is limited information concerning the plant model, and the existing controller. The approach is applied to the design of an adaptive autopilot for a guided munition. Design of a neural network adaptive control that ensures asymptotically stable tracking performance is also addressed.

  6. Complexity and Pilot Workload Metrics for the Evaluation of Adaptive Flight Controls on a Full Scale Piloted Aircraft

    NASA Technical Reports Server (NTRS)

    Hanson, Curt; Schaefer, Jacob; Burken, John J.; Larson, David; Johnson, Marcus

    2014-01-01

    Flight research has shown the effectiveness of adaptive flight controls for improving aircraft safety and performance in the presence of uncertainties. The National Aeronautics and Space Administration's (NASA)'s Integrated Resilient Aircraft Control (IRAC) project designed and conducted a series of flight experiments to study the impact of variations in adaptive controller design complexity on performance and handling qualities. A novel complexity metric was devised to compare the degrees of simplicity achieved in three variations of a model reference adaptive controller (MRAC) for NASA's F-18 (McDonnell Douglas, now The Boeing Company, Chicago, Illinois) Full-Scale Advanced Systems Testbed (Gen-2A) aircraft. The complexity measures of these controllers are also compared to that of an earlier MRAC design for NASA's Intelligent Flight Control System (IFCS) project and flown on a highly modified F-15 aircraft (McDonnell Douglas, now The Boeing Company, Chicago, Illinois). Pilot comments during the IRAC research flights pointed to the importance of workload on handling qualities ratings for failure and damage scenarios. Modifications to existing pilot aggressiveness and duty cycle metrics are presented and applied to the IRAC controllers. Finally, while adaptive controllers may alleviate the effects of failures or damage on an aircraft's handling qualities, they also have the potential to introduce annoying changes to the flight dynamics or to the operation of aircraft systems. A nuisance rating scale is presented for the categorization of nuisance side-effects of adaptive controllers.

  7. Dietary and Flight Energetic Adaptations in a Salivary Gland Transcriptome of an Insectivorous Bat

    PubMed Central

    Phillips, Carleton J.; Phillips, Caleb D.; Goecks, Jeremy; Lessa, Enrique P.; Sotero-Caio, Cibele G.; Tandler, Bernard; Gannon, Michael R.; Baker, Robert J.

    2014-01-01

    We hypothesized that evolution of salivary gland secretory proteome has been important in adaptation to insectivory, the most common dietary strategy among Chiroptera. A submandibular salivary gland (SMG) transcriptome was sequenced for the little brown bat, Myotis lucifugus. The likely secretory proteome of 23 genes included seven (RETNLB, PSAP, CLU, APOE, LCN2, C3, CEL) related to M. lucifugus insectivorous diet and metabolism. Six of the secretory proteins probably are endocrine, whereas one (CEL) most likely is exocrine. The encoded proteins are associated with lipid hydrolysis, regulation of lipid metabolism, lipid transport, and insulin resistance. They are capable of processing exogenous lipids for flight metabolism while foraging. Salivary carboxyl ester lipase (CEL) is thought to hydrolyze insect lipophorins, which probably are absorbed across the gastric mucosa during feeding. The other six proteins are predicted either to maintain these lipids at high blood concentrations or to facilitate transport and uptake by flight muscles. Expression of these seven genes and coordinated secretion from a single organ is novel to this insectivorous bat, and apparently has evolved through instances of gene duplication, gene recruitment, and nucleotide selection. Four of the recruited genes are single-copy in the Myotis genome, whereas three have undergone duplication(s) with two of these genes exhibiting evolutionary ‘bursts’ of duplication resulting in multiple paralogs. Evidence for episodic directional selection was found for six of seven genes, reinforcing the conclusion that the recruited genes have important roles in adaptation to insectivory and the metabolic demands of flight. Intragenic frequencies of mobile- element-like sequences differed from frequencies in the whole M. lucifugus genome. Differences among recruited genes imply separate evolutionary trajectories and that adaptation was not a single, coordinated event. PMID:24454705

  8. F-8C adaptive flight control extensions. [for maximum likelihood estimation

    NASA Technical Reports Server (NTRS)

    Stein, G.; Hartmann, G. L.

    1977-01-01

    An adaptive concept which combines gain-scheduled control laws with explicit maximum likelihood estimation (MLE) identification to provide the scheduling values is described. The MLE algorithm was improved by incorporating attitude data, estimating gust statistics for setting filter gains, and improving parameter tracking during changing flight conditions. A lateral MLE algorithm was designed to improve true air speed and angle of attack estimates during lateral maneuvers. Relationships between the pitch axis sensors inherent in the MLE design were examined and used for sensor failure detection. Design details and simulation performance are presented for each of the three areas investigated.

  9. Flight Test Comparison of Different Adaptive Augmentations for Fault Tolerant Control Laws for a Modified F-15 Aircraft

    NASA Technical Reports Server (NTRS)

    Burken, John J.; Hanson, Curtis E.; Lee, James A.; Kaneshige, John T.

    2009-01-01

    This report describes the improvements and enhancements to a neural network based approach for directly adapting to aerodynamic changes resulting from damage or failures. This research is a follow-on effort to flight tests performed on the NASA F-15 aircraft as part of the Intelligent Flight Control System research effort. Previous flight test results demonstrated the potential for performance improvement under destabilizing damage conditions. Little or no improvement was provided under simulated control surface failures, however, and the adaptive system was prone to pilot-induced oscillations. An improved controller was designed to reduce the occurrence of pilot-induced oscillations and increase robustness to failures in general. This report presents an analysis of the neural networks used in the previous flight test, the improved adaptive controller, and the baseline case with no adaptation. Flight test results demonstrate significant improvement in performance by using the new adaptive controller compared with the previous adaptive system and the baseline system for control surface failures.

  10. Rule-based mechanisms of learning for intelligent adaptive flight control

    NASA Technical Reports Server (NTRS)

    Handelman, David A.; Stengel, Robert F.

    1990-01-01

    How certain aspects of human learning can be used to characterize learning in intelligent adaptive control systems is investigated. Reflexive and declarative memory and learning are described. It is shown that model-based systems-theoretic adaptive control methods exhibit attributes of reflexive learning, whereas the problem-solving capabilities of knowledge-based systems of artificial intelligence are naturally suited for implementing declarative learning. Issues related to learning in knowledge-based control systems are addressed, with particular attention given to rule-based systems. A mechanism for real-time rule-based knowledge acquisition is suggested, and utilization of this mechanism within the context of failure diagnosis for fault-tolerant flight control is demonstrated.

  11. Digital adaptive model following flight control. [using fighter aircraft mathematical model-following algorithm

    NASA Technical Reports Server (NTRS)

    Alag, G. S.; Kaufman, H.

    1974-01-01

    Simple mechanical linkages are often unable to cope with the many control problems associated with high performance aircraft maneuvering over a wide flight envelope. One procedure for retaining uniform handling qualities over such an envelope is to implement a digital adaptive controller. Towards such an implementation an explicit adaptive controller, which makes direct use of online parameter identification, has been developed and applied to the linearized equations of motion for a typical fighter aircraft. The system is composed of an online weighted least squares identifier, a Kalman state filter, and a single stage real model following control law. The corresponding control gains are readily adjustable in accordance with parameter changes to ensure asymptotic stability if the conditions for perfect model following are satisfied and stability in the sense of boundedness otherwise.

  12. Sensory-Motor Adaptation to Space Flight: Human Balance Control and Artificial Gravity

    NASA Technical Reports Server (NTRS)

    Paloski, William H.

    2004-01-01

    Gravity, which is sensed directly by the otolith organs and indirectly by proprioceptors and exteroceptors, provides the CNS a fundamental reference for estimating spatial orientation and coordinating movements in the terrestrial environment. The sustained absence of gravity during orbital space flight creates a unique environment that cannot be reproduced on Earth. Loss of this fundamental CNS reference upon insertion into orbit triggers neuro-adaptive processes that optimize performance for the microgravity environment, while its reintroduction upon return to Earth triggers neuro-adaptive processes that return performance to terrestrial norms. Five pioneering symposia on The Role of the Vestibular Organs in the Exploration of Space were convened between 1965 and 1970. These innovative meetings brought together the top physicians, physiologists, and engineers in the vestibular field to discuss and debate the challenges associated with human vestibular system adaptation to the then novel environment of space flight. These highly successful symposia addressed the perplexing problem of how to understand and ameliorate the adverse physiological effects on humans resulting from the reduction of gravitational stimulation of the vestibular receptors in space. The series resumed in 2002 with the Sixth Symposium, which focused on the microgravity environment as an essential tool for the study of fundamental vestibular functions. The three day meeting included presentations on historical perspectives, vestibular neurobiology, neurophysiology, neuroanatomy, neurotransmitter systems, theoretical considerations, spatial orientation, psychophysics, motor integration, adaptation, autonomic function, space motion sickness, clinical issues, countermeasures, and rehabilitation. Scientists and clinicians entered into lively exchanges on how to design and perform mutually productive research and countermeasure development projects in the future. The problems posed by long duration

  13. Flight Test of an Adaptive Configuration Optimization System for Transport Aircraft

    NASA Technical Reports Server (NTRS)

    Gilyard, Glenn B.; Georgie, Jennifer; Barnicki, Joseph S.

    1999-01-01

    A NASA Dryden Flight Research Center program explores the practical application of real-time adaptive configuration optimization for enhanced transport performance on an L-1011 aircraft. This approach is based on calculation of incremental drag from forced-response, symmetric, outboard aileron maneuvers. In real-time operation, the symmetric outboard aileron deflection is directly optimized, and the horizontal stabilator and angle of attack are indirectly optimized. A flight experiment has been conducted from an onboard research engineering test station, and flight research results are presented herein. The optimization system has demonstrated the capability of determining the minimum drag configuration of the aircraft in real time. The drag-minimization algorithm is capable of identifying drag to approximately a one-drag-count level. Optimizing the symmetric outboard aileron position realizes a drag reduction of 2-3 drag counts (approximately 1 percent). Algorithm analysis of maneuvers indicate that two-sided raised-cosine maneuvers improve definition of the symmetric outboard aileron drag effect, thereby improving analysis results and consistency. Ramp maneuvers provide a more even distribution of data collection as a function of excitation deflection than raised-cosine maneuvers provide. A commercial operational system would require airdata calculations and normal output of current inertial navigation systems; engine pressure ratio measurements would be optional.

  14. Flight control design using a blend of modern nonlinear adaptive and robust techniques

    NASA Astrophysics Data System (ADS)

    Yang, Xiaolong

    In this dissertation, the modern control techniques of feedback linearization, mu synthesis, and neural network based adaptation are used to design novel control laws for two specific applications: F/A-18 flight control and reusable launch vehicle (an X-33 derivative) entry guidance. For both applications, the performance of the controllers is assessed. As a part of a NASA Dryden program to develop and flight test experimental controllers for an F/A-18 aircraft, a novel method of combining mu synthesis and feedback linearization is developed to design longitudinal and lateral-directional controllers. First of all, the open-loop and closed-loop dynamics of F/A-18 are investigated. The production F/A-18 controller as well as the control distribution mechanism are studied. The open-loop and closed-loop handling qualities of the F/A-18 are evaluated using low order transfer functions. Based on this information, a blend of robust mu synthesis and feedback linearization is used to design controllers for a low dynamic pressure envelope of flight conditions. For both the longitudinal and the lateral-directional axes, a robust linear controller is designed for a trim point in the center of the envelope. Then by including terms to cancel kinematic nonlinearities and variations in the aerodynamic forces and moments over the flight envelope, a complete nonlinear controller is developed. In addition, to compensate for the model uncertainty, linearization error and variations between operating points, neural network based adaptation is added to the designed longitudinal controller. The nonlinear simulations, robustness and handling qualities analysis indicate that the performance is similar to or better than that for the production F/A-18 controllers. When the dynamic pressure is very low, the performance of both the experimental and the production flight controllers is degraded, but Level I handling qualities are still achieved. A new generation of Reusable Launch Vehicles

  15. Launch Vehicle Manual Steering with Adaptive Augmenting Control:In-Flight Evaluations of Adverse Interactions Using a Piloted Aircraft

    NASA Technical Reports Server (NTRS)

    Hanson, Curt; Miller, Chris; Wall, John H.; VanZwieten, Tannen S.; Gilligan, Eric T.; Orr, Jeb S.

    2015-01-01

    An Adaptive Augmenting Control (AAC) algorithm for the Space Launch System (SLS) has been developed at the Marshall Space Flight Center (MSFC) as part of the launch vehicle's baseline flight control system. A prototype version of the SLS flight control software was hosted on a piloted aircraft at the Armstrong Flight Research Center to demonstrate the adaptive controller on a full-scale realistic application in a relevant flight environment. Concerns regarding adverse interactions between the adaptive controller and a potential manual steering mode were also investigated by giving the pilot trajectory deviation cues and pitch rate command authority, which is the subject of this paper. Two NASA research pilots flew a total of 25 constant pitch rate trajectories using a prototype manual steering mode with and without adaptive control, evaluating six different nominal and off-nominal test case scenarios. Pilot comments and PIO ratings were given following each trajectory and correlated with aircraft state data and internal controller signals post-flight.

  16. Flight Testing of the Space Launch System (SLS) Adaptive Augmenting Control (AAC) Algorithm on an F/A-18

    NASA Technical Reports Server (NTRS)

    Dennehy, Cornelius J.; VanZwieten, Tannen S.; Hanson, Curtis E.; Wall, John H.; Miller, Chris J.; Gilligan, Eric T.; Orr, Jeb S.

    2014-01-01

    The Marshall Space Flight Center (MSFC) Flight Mechanics and Analysis Division developed an adaptive augmenting control (AAC) algorithm for launch vehicles that improves robustness and performance on an as-needed basis by adapting a classical control algorithm to unexpected environments or variations in vehicle dynamics. This was baselined as part of the Space Launch System (SLS) flight control system. The NASA Engineering and Safety Center (NESC) was asked to partner with the SLS Program and the Space Technology Mission Directorate (STMD) Game Changing Development Program (GCDP) to flight test the AAC algorithm on a manned aircraft that can achieve a high level of dynamic similarity to a launch vehicle and raise the technology readiness of the algorithm early in the program. This document reports the outcome of the NESC assessment.

  17. Behavioural Adaptation to diminished Gravity in Fish - a Parabolic Aircraft Flight Study

    NASA Astrophysics Data System (ADS)

    Forster, A.; Anken, R.; Hilbig, R.

    During the micro gravity phases in the course of parabolic aircraft flights PFs some fish of a given batch were frequently shown to exhibit sensorimotor disorders in terms of revealing so-called looping responses LR or spinning movements SM both forms of motion sickness a kinetosis In order to gain some insights into the time-course of the behavioural adaptation towards diminished gravity in total 272 larval cichlid fish Oreochromis mossambicus were subjected to PFs and their respective behaviour was monitored With the onset of the first parabola P1 15 9 of the animals revealed a kinetotic behaviour whereas kinetoses were shown in 6 5 1 5 and 1 of the animals in P5 P10 and P15 With P20 the animals had adapted completely 0 swimming kinetotically Since the relative decrease of kinetotic animals was especially prominent from P5 to P10 a detailed analysis of the behaviour was undertaken Regarding SM a ratio of 2 9 in P5 decreased to 0 5 in P10 Virtually all individuals showing a SM in P5 had regained a normal behaviour with P10 The SM animals in P10 had all exhibited a normal swimming behaviour in P5 The ratio of LR-fish also decreased from P5 3 6 to P10 1 0 In contrast to the findings regarding SM numerous LM specimens did not regain a normal postural control and only very few animals behaving normally in P5 began to sport a LM behaviour by P10 Summarizing most kinetotic animals rapidly adapted to diminished gravity but few individual fish who swam normally at the beginning of the flights may loose sensorimotor control

  18. Improving Sensorimotor Adaptation Following Long Duration Space Flight by Enhancing Vestibular Information Transfer

    NASA Technical Reports Server (NTRS)

    Mulavara, A. P.; Kofman, I. S.; De Dios, Y. E; Galvan, R.; Goel, R.; Miller, C.; Peters, B.; Cohen, H. S.; Jeevarajan, J.; Reschke, M.; Wood, S.; Bergquist, F.; Seidler, R. D.; Bloomberg, J. J.

    2014-01-01

    Crewmember adapted to the microgravity state may need to egress the vehicle within a few minutes for safety and operational reasons after gravitational transitions. The transition from one sensorimotor state to another consists of two main mechanisms: strategic and plastic-adaptive and have been demonstrated in astronauts returning after long duration space flight. Strategic modifications represent "early adaptation" - immediate and transitory changes in control that are employed to deal with short-term changes in the environment. If these modifications are prolonged then plastic-adaptive changes are evoked that modify central nervous system function, automating new behavioral responses. More importantly, this longer term adaptive recovery mechanism was significantly associated with their strategic ability to recover on the first day after return to Earth G. We are developing a method based on stochastic resonance to enhance information transfer by improving the brain's ability to detect vestibular signals (Vestibular Stochastic Resonance, VSR) especially when combined with balance training exercises such as sensorimotor adaptability (SA) training for rapid improvement in functional skill, for standing and mobility. This countermeasure to improve detection of vestibular signals is a stimulus delivery system that is wearable/portable providing low imperceptible levels of white noise based binaural bipolar electrical stimulation of the vestibular system (stochastic vestibular stimulation). To determine efficacy of vestibular stimulation on physiological and perceptual responses during otolith-canal conflicts and dynamic perturbations we have conducted a series of studies: We have shown that imperceptible binaural bipolar electrical stimulation of the vestibular system across the mastoids enhances balance performance in the mediolateral (ML) plane while standing on an unstable surface. We have followed up on the previous study showing VSR stimulation improved balance

  19. Adapted ECC ozonesonde for long-duration flights aboard boundary-layer pressurised balloons

    NASA Astrophysics Data System (ADS)

    Gheusi, François; Durand, Pierre; Verdier, Nicolas; Dulac, François; Attié, Jean-Luc; Commun, Philippe; Barret, Brice; Basdevant, Claude; Clenet, Antoine; Derrien, Solène; Doerenbecher, Alexis; El Amraoui, Laaziz; Fontaine, Alain; Hache, Emeric; Jambert, Corinne; Jaumouillé, Elodie; Meyerfeld, Yves; Roblou, Laurent; Tocquer, Flore

    2016-12-01

    Since the 1970s, the French space agency CNES has developed boundary-layer pressurised balloons (BLPBs) with the capability to transport lightweight scientific payloads at isopycnic level and offer a quasi-Lagrangian sampling of the lower atmosphere over very long distances and durations (up to several weeks).

    Electrochemical concentration cell (ECC) ozonesondes are widely used under small sounding balloons. However, their autonomy is limited to a few hours owing to power consumption and electrolyte evaporation. An adaptation of the ECC sonde has been developed specifically for long-duration BLPB flights. Compared to conventional ECC sondes, the main feature is the possibility of programming periodic measurement sequences (with possible remote control during the flight). To increase the ozonesonde autonomy, the strategy has been adopted of short measurement sequences (2-3 min) regularly spaced in time (e.g. every 15 min). The rest of the time, the sonde pump is turned off. Results of preliminary ground-based tests are first presented. In particular, the sonde was able to provide correct ozone concentrations against a reference UV-absorption ozone analyser every 15 min for 4 days. Then we illustrate results from 16 BLBP flights launched over the western Mediterranean during three summer field campaigns of the ChArMEx project (http://charmex.lsce.ipsl.fr): TRAQA in 2012, and ADRIMED and SAFMED in 2013. BLPB drifting altitudes were in the range 0.25-3.2 km. The longest flight lasted more than 32 h and covered more than 1000 km. Satisfactory data were obtained when compared to independent ozone measurements close in space and time. The quasi-Lagrangian measurements allowed a first look at ozone diurnal evolution in the marine boundary layer as well as in the lower free troposphere. During some flight segments, there was indication of photochemical ozone production in the marine boundary layer or even

  20. [Individual peculiarities of adaptation to long-term space flights: 24-hour heart rhythm monitoring

    NASA Technical Reports Server (NTRS)

    Baevskii, R. M.; Bogomolov, V. V.; Gol'dberger, A. L.; Nikulina, G. A.; Charl'z, D. B.; Goldberger, A. L. (Principal Investigator); Charles, J. B. (Principal Investigator)

    2000-01-01

    Presented are results of studying 24-hr variability of the cardiac rhythm which characterizes individual difference in reactions of two crew members to the same set of stresses during a 115-day MIR mission. Spacelab (USA) cardiorecorders were used. Data of monitoring revealed significantly different baseline health statuses of the cosmonauts. These functional differences were also observed in the mission. In one of the cosmonauts, the cardiac regulation changed over to a more economic functioning with the autonomous balance shifted towards enhanced sympathetic activity. After 2-3 months on mission he had almost recovered pre-launch level of regulation. In the other, the regulatory system was appreciably strained at the beginning of the mission as compared with preflight baseline. Later on, on flight months 2-3, this strain kept growing till a drastic depletion of the functional reserve. On return to Earth, this was manifested by a strong stress reaction with a sharp decline in power of high-frequency and grow in power of very low frequency components of the heart rhythm. The data suggest that adaptation to space flight and reactions in the readaptation period are dependent on initial health status of crew members, and functional reserve.

  1. [Individual peculiarities of adaptation to long-term space flights: 24-hour heart rhythm monitoring].

    PubMed

    Baevskiĭ, R M; Bogomolov, V V; Gol'dberger, A L; Nikulina, G A; Charl'z, D B

    2000-01-01

    Presented are results of studying 24-hr variability of the cardiac rhythm which characterizes individual difference in reactions of two crew members to the same set of stresses during a 115-day MIR mission. Spacelab (USA) cardiorecorders were used. Data of monitoring revealed significantly different baseline health statuses of the cosmonauts. These functional differences were also observed in the mission. In one of the cosmonauts, the cardiac regulation changed over to a more economic functioning with the autonomous balance shifted towards enhanced sympathetic activity. After 2-3 months on mission he had almost recovered pre-launch level of regulation. In the other, the regulatory system was appreciably strained at the beginning of the mission as compared with preflight baseline. Later on, on flight months 2-3, this strain kept growing till a drastic depletion of the functional reserve. On return to Earth, this was manifested by a strong stress reaction with a sharp decline in power of high-frequency and grow in power of very low frequency components of the heart rhythm. The data suggest that adaptation to space flight and reactions in the readaptation period are dependent on initial health status of crew members, and functional reserve.

  2. Development and Flight Testing of an Adaptive Vehicle Health-Monitoring Architecture

    NASA Technical Reports Server (NTRS)

    Woodard, Stanley E.; Coffey, Neil C.; Gonzalez, Guillermo A.; Taylor, B. Douglas; Brett, Rube R.; Woodman, Keith L.; Weathered, Brenton W.; Rollins, Courtney H.

    2002-01-01

    On going development and testing of an adaptable vehicle health-monitoring architecture is presented. The architecture is being developed for a fleet of vehicles. It has three operational levels: one or more remote data acquisition units located throughout the vehicle; a command and control unit located within the vehicle, and, a terminal collection unit to collect analysis results from all vehicles. Each level is capable of performing autonomous analysis with a trained expert system. The expert system is parameterized, which makes it adaptable to be trained to both a user's subject reasoning and existing quantitative analytic tools. Communication between all levels is done with wireless radio frequency interfaces. The remote data acquisition unit has an eight channel programmable digital interface that allows the user discretion for choosing type of sensors; number of sensors, sensor sampling rate and sampling duration for each sensor. The architecture provides framework for a tributary analysis. All measurements at the lowest operational level are reduced to provide analysis results necessary to gauge changes from established baselines. These are then collected at the next level to identify any global trends or common features from the prior level. This process is repeated until the results are reduced at the highest operational level. In the framework, only analysis results are forwarded to the next level to reduce telemetry congestion. The system's remote data acquisition hardware and non-analysis software have been flight tested on the NASA Langley B757's main landing gear. The flight tests were performed to validate the following: the wireless radio frequency communication capabilities of the system, the hardware design, command and control; software operation and, data acquisition, storage and retrieval.

  3. Physiological Observations and Omics to Develop Personalized Sensormotor Adaptability Countermeasures Using Bed Rest and Space Flight Data

    NASA Technical Reports Server (NTRS)

    Mulavara, A. P.; Seidler, R. D.; Feiveson, A.; Oddsson, L.; Zanello, S.; Oman, C. M.; Ploutz-Snyder, L.; Peters, B.; Cohen, H. S.; Reschke, M.; Wood, S.; Bloomberg, J. J.

    2014-01-01

    Astronauts experience sensorimotor disturbances during the initial exposure to microgravity and during the re-adapation phase following a return to an earth-gravitational environment. These alterations may disrupt the ability to perform mission critical functional tasks requiring ambulation, manual control and gaze stability. Interestingly, astronauts who return from space flight show substantial differences in their abilities to readapt to a gravitational environment. The ability to predict the manner and degree to which individual astronauts would be affected would improve the effectiveness of countermeasure training programs designed to enhance sensorimotor adaptability. For such an approach to succeed, we must develop predictive measures of sensorimotor adaptability that will allow us to foresee, before actual space flight, which crewmembers are likely to experience the greatest challenges to their adaptive capacities. The goals of this project are to identify and characterize this set of predictive measures that include: 1) behavioral tests to assess sensory bias and adaptability quantified using both strategic and plastic-adaptive responses; 2) imaging to determine individual brain morphological and functional features using structural magnetic resonance imaging (MRI), diffusion tensor imaging, resting state functional connectivity MRI, and sensorimotor adaptation task-related functional brain activation; 3) genotype markers for genetic polymorphisms in Catechol-O-Methyl Transferase, Dopamine Receptor D2, Brain-derived neurotrophic factor and genetic polymorphism of alpha2-adrenergic receptor that play a role in the neural pathways underlying sensorimotor adaptation. We anticipate these predictive measures will be significantly correlated with individual differences in sensorimotor adaptability after long-duration space flight and an analog bed rest environment. We will be conducting a retrospective study leveraging data already collected from relevant

  4. Functional Task Test: 3. Skeletal Muscle Performance Adaptations to Space Flight

    NASA Technical Reports Server (NTRS)

    Ryder, Jeffrey W.; Wickwire, P. J.; Buxton, R. E.; Bloomberg, J. J.; Ploutz-Snyder, L.

    2011-01-01

    The functional task test is a multi-disciplinary study investigating how space-flight induced changes to physiological systems impacts functional task performance. Impairment of neuromuscular function would be expected to negatively affect functional performance of crewmembers following exposure to microgravity. This presentation reports the results for muscle performance testing in crewmembers. Functional task performance will be presented in the abstract "Functional Task Test 1: sensory motor adaptations associated with postflight alternations in astronaut functional task performance." METHODS: Muscle performance measures were obtained in crewmembers before and after short-duration space flight aboard the Space Shuttle and long-duration International Space Station (ISS) missions. The battery of muscle performance tests included leg press and bench press measures of isometric force, isotonic power and total work. Knee extension was used for the measurement of central activation and maximal isometric force. Upper and lower body force steadiness control were measured on the bench press and knee extension machine, respectively. Tests were implemented 60 and 30 days before launch, on landing day (Shuttle crew only), and 6, 10 and 30 days after landing. Seven Space Shuttle crew and four ISS crew have completed the muscle performance testing to date. RESULTS: Preliminary results for Space Shuttle crew reveal significant reductions in the leg press performance metrics of maximal isometric force, power and total work on R+0 (p<0.05). Bench press total work was also significantly impaired, although maximal isometric force and power were not significantly affected. No changes were noted for measurements of central activation or force steadiness. Results for ISS crew were not analyzed due to the current small sample size. DISCUSSION: Significant reductions in lower body muscle performance metrics were observed in returning Shuttle crew and these adaptations are likely

  5. Stability Assessment and Tuning of an Adaptively Augmented Classical Controller for Launch Vehicle Flight Control

    NASA Technical Reports Server (NTRS)

    VanZwieten, Tannen; Zhu, J. Jim; Adami, Tony; Berry, Kyle; Grammar, Alex; Orr, Jeb S.; Best, Eric A.

    2014-01-01

    Recently, a robust and practical adaptive control scheme for launch vehicles [ [1] has been introduced. It augments a classical controller with a real-time loop-gain adaptation, and it is therefore called Adaptive Augmentation Control (AAC). The loop-gain will be increased from the nominal design when the tracking error between the (filtered) output and the (filtered) command trajectory is large; whereas it will be decreased when excitation of flex or sloshing modes are detected. There is a need to determine the range and rate of the loop-gain adaptation in order to retain (exponential) stability, which is critical in vehicle operation, and to develop some theoretically based heuristic tuning methods for the adaptive law gain parameters. The classical launch vehicle flight controller design technics are based on gain-scheduling, whereby the launch vehicle dynamics model is linearized at selected operating points along the nominal tracking command trajectory, and Linear Time-Invariant (LTI) controller design techniques are employed to ensure asymptotic stability of the tracking error dynamics, typically by meeting some prescribed Gain Margin (GM) and Phase Margin (PM) specifications. The controller gains at the design points are then scheduled, tuned and sometimes interpolated to achieve good performance and stability robustness under external disturbances (e.g. winds) and structural perturbations (e.g. vehicle modeling errors). While the GM does give a bound for loop-gain variation without losing stability, it is for constant dispersions of the loop-gain because the GM is based on frequency-domain analysis, which is applicable only for LTI systems. The real-time adaptive loop-gain variation of the AAC effectively renders the closed-loop system a time-varying system, for which it is well-known that the LTI system stability criterion is neither necessary nor sufficient when applying to a Linear Time-Varying (LTV) system in a frozen-time fashion. Therefore, a

  6. Development and Flight Testing of an Adaptable Vehicle Health-Monitoring Architecture

    NASA Technical Reports Server (NTRS)

    Woodard, Stanley E.; Coffey, Neil C.; Gonzalez, Guillermo A.; Woodman, Keith L.; Weathered, Brenton W.; Rollins, Courtney H.; Taylor, B. Douglas; Brett, Rube R.

    2003-01-01

    Development and testing of an adaptable wireless health-monitoring architecture for a vehicle fleet is presented. It has three operational levels: one or more remote data acquisition units located throughout the vehicle; a command and control unit located within the vehicle; and a terminal collection unit to collect analysis results from all vehicles. Each level is capable of performing autonomous analysis with a trained adaptable expert system. The remote data acquisition unit has an eight channel programmable digital interface that allows the user discretion for choosing type of sensors; number of sensors, sensor sampling rate, and sampling duration for each sensor. The architecture provides framework for a tributary analysis. All measurements at the lowest operational level are reduced to provide analysis results necessary to gauge changes from established baselines. These are then collected at the next level to identify any global trends or common features from the prior level. This process is repeated until the results are reduced at the highest operational level. In the framework, only analysis results are forwarded to the next level to reduce telemetry congestion. The system's remote data acquisition hardware and non-analysis software have been flight tested on the NASA Langley B757's main landing gear.

  7. Self-organizing radial basis function networks for adaptive flight control and aircraft engine state estimation

    NASA Astrophysics Data System (ADS)

    Shankar, Praveen

    The performance of nonlinear control algorithms such as feedback linearization and dynamic inversion is heavily dependent on the fidelity of the dynamic model being inverted. Incomplete or incorrect knowledge of the dynamics results in reduced performance and may lead to instability. Augmenting the baseline controller with approximators which utilize a parametrization structure that is adapted online reduces the effect of this error between the design model and actual dynamics. However, currently existing parameterizations employ a fixed set of basis functions that do not guarantee arbitrary tracking error performance. To address this problem, we develop a self-organizing parametrization structure that is proven to be stable and can guarantee arbitrary tracking error performance. The training algorithm to grow the network and adapt the parameters is derived from Lyapunov theory. In addition to growing the network of basis functions, a pruning strategy is incorporated to keep the size of the network as small as possible. This algorithm is implemented on a high performance flight vehicle such as F-15 military aircraft. The baseline dynamic inversion controller is augmented with a Self-Organizing Radial Basis Function Network (SORBFN) to minimize the effect of the inversion error which may occur due to imperfect modeling, approximate inversion or sudden changes in aircraft dynamics. The dynamic inversion controller is simulated for different situations including control surface failures, modeling errors and external disturbances with and without the adaptive network. A performance measure of maximum tracking error is specified for both the controllers a priori. Excellent tracking error minimization to a pre-specified level using the adaptive approximation based controller was achieved while the baseline dynamic inversion controller failed to meet this performance specification. The performance of the SORBFN based controller is also compared to a fixed RBF network

  8. A Reusable and Adaptable Software Architecture for Embedded Space Flight System: The Core Flight Software System (CFS)

    NASA Technical Reports Server (NTRS)

    Wilmot, Jonathan

    2005-01-01

    The contents include the following: High availability. Hardware is in harsh environment. Flight processor (constraints) very widely due to power and weight constraints. Software must be remotely modifiable and still operate while changes are being made. Many custom one of kind interfaces for one of a kind missions. Sustaining engineering. Price of failure is high, tens to hundreds of millions of dollars.

  9. Implementation of an Adaptive Controller System from Concept to Flight Test

    NASA Technical Reports Server (NTRS)

    Larson, Richard R.; Burken, John J.; Butler, Bradley S.; Yokum, Steve

    2009-01-01

    tunable metrics for the FL are (1) window size, (2) drift rate, and (3) persistence counter. Ultimate range limits are also included in case the NN command should drift slowly to a limit value that would cause the FL to be defeated. The FL has proven to work as intended. Both high-g transients and excessive structural loads are controlled with NN hard-over commands. This presentation discusses the FL design features and presents test cases. Simulation runs are included to illustrate the dramatic improvement made to the control of NN hard-over effects. A mission control room display from a flight playback is presented to illustrate the neural net fault display representation. The FL is very adaptable to various requirements and is independent of flight condition. It should be considered as a cost-effective safety monitor to control single-string inputs in general.

  10. Evaluation of the Hinge Moment and Normal Force Aerodynamic Loads from a Seamless Adaptive Compliant Trailing Edge Flap in Flight

    NASA Technical Reports Server (NTRS)

    Miller, Eric J.; Cruz, Josue; Lung, Shun-Fat; Kota, Sridhar; Ervin, Gregory; Lu, Kerr-Jia; Flick, Pete

    2016-01-01

    A seamless adaptive compliant trailing edge (ACTE) flap was demonstrated in flight on a Gulfstream III aircraft at the NASA Armstrong Flight Research Center. The trailing edge flap was deflected between minus 2 deg up and plus 30 deg down in flight. The safety-of-flight parameters for the ACTE flap experiment require that flap-to-wing interface loads be sensed and monitored in real time to ensure that the structural load limits of the wing are not exceeded. The attachment fittings connecting the flap to the aircraft wing rear spar were instrumented with strain gages and calibrated using known loads for measuring hinge moment and normal force loads in flight. The safety-of-flight parameters for the ACTE flap experiment require that flap-to-wing interface loads be sensed and monitored in real time to ensure that the structural load limits of the wing are not exceeded. The attachment fittings connecting the flap to the aircraft wing rear spar were instrumented with strain gages and calibrated using known loads for measuring hinge moment and normal force loads in flight. The interface hardware instrumentation layout and load calibration are discussed. Twenty-one applied calibration test load cases were developed for each individual fitting. The 2-sigma residual errors for the hinge moment was calculated to be 2.4 percent, and for normal force was calculated to be 7.3 percent. The hinge moment and normal force generated by the ACTE flap with a hinge point located at 26-percent wing chord were measured during steady state and symmetric pitch maneuvers. The loads predicted from analysis were compared to the loads observed in flight. The hinge moment loads showed good agreement with the flight loads while the normal force loads calculated from analysis were over-predicted by approximately 20 percent. Normal force and hinge moment loads calculated from the pressure sensors located on the ACTE showed good agreement with the loads calculated from the installed strain gages.

  11. Current trends in the usage of the Adaptability Rating for Military Aviation (ARMA) among USAF flight surgeons.

    PubMed

    Verdone, R D; Sipes, W; Miles, R

    1993-12-01

    The Adaptability Rating for Military Aviation (ARMA) is that portion of the initial flight physical that assesses an aviator candidate's motivation for and potential adaptability toward an aviation career. A survey was mailed to all USAF operational flight surgeons in the continental U.S. to describe the frequency and distribution of ARMA usage and attitudes. Descriptive statistics suggest that the ARMA is used suboptimally in accordance with current USAF regulation. ARMA training, flight surgeon satisfaction and lack of regulation clarity are described and discussed. More flight surgeons are dissatisfied with the ARMA than are satisfied, and the regulation is perceived as unclear in the area of final disposition for candidates with equivocal ARMA's. A post-hoc analysis to rule out the influences of rank, gender, experience and residency training was performed. Residency training in Aerospace Medicine is beneficial in terms of doing an ARMA, when required, and covering recommended areas. Females and those with less than 1 year experience perform an ARMA more frequently than males and experienced flight surgeons. Despite the limitations of the current ARMA, it should not be abandoned. Recommendations to improve it are provided. Doing better ARMA's can lead to decreased illness, injury, accidents, and attrition.

  12. L1 Adaptive Control Law for Flexible Space Launch Vehicle and Proposed Plan for Flight Test Validation

    NASA Technical Reports Server (NTRS)

    Kharisov, Evgeny; Gregory, Irene M.; Cao, Chengyu; Hovakimyan, Naira

    2008-01-01

    This paper explores application of the L1 adaptive control architecture to a generic flexible Crew Launch Vehicle (CLV). Adaptive control has the potential to improve performance and enhance safety of space vehicles that often operate in very unforgiving and occasionally highly uncertain environments. NASA s development of the next generation space launch vehicles presents an opportunity for adaptive control to contribute to improved performance of this statically unstable vehicle with low damping and low bending frequency flexible dynamics. In this paper, we consider the L1 adaptive output feedback controller to control the low frequency structural modes and propose steps to validate the adaptive controller performance utilizing one of the experimental test flights for the CLV Ares-I Program.

  13. The three-dimensional flight of red-footed boobies: adaptations to foraging in a tropical environment?

    PubMed

    Weimerskirch, H; Le Corre, M; Ropert-Coudert, Y; Kato, A; Marsac, F

    2005-01-07

    In seabirds a broad variety of morphologies, flight styles and feeding methods exist as an adaptation to optimal foraging in contrasted marine environments for a wide variety of prey types. Because of the low productivity of tropical waters it is expected that specific flight and foraging techniques have been selected there, but very few data are available. By using five different types of high-precision miniaturized logger (global positioning systems, accelerometers, time depth recorders, activity recorders, altimeters) we studied the way a seabird is foraging over tropical waters. Red-footed boobies are foraging in the day, never foraging at night, probably as a result of predation risks. They make extensive use of wind conditions, flying preferentially with crosswinds at median speed of 38 km h(-1), reaching highest speeds with tail winds. They spent 66% of the foraging trip in flight, using a flap-glide flight, and gliding 68% of the flight. Travelling at low costs was regularly interrupted by extremely active foraging periods where birds are very frequently touching water for landing, plunge diving or surface diving (30 landings h(-1)). Dives were shallow (maximum 2.4 m) but frequent (4.5 dives h(-1)), most being plunge dives. While chasing for very mobile prey like flying fishes, boobies have adopted a very active and specific hunting behaviour, but the use of wind allows them to reduce travelling cost by their extensive use of gliding. During the foraging and travelling phases birds climb regularly to altitudes of 20-50 m to spot prey or congeners. During the final phase of the flight, they climb to high altitudes, up to 500 m, probably to avoid attacks by frigatebirds along the coasts. This study demonstrates the use by boobies of a series of very specific flight and activity patterns that have probably been selected as adaptations to the conditions of tropical waters.

  14. The three-dimensional flight of red-footed boobies: adaptations to foraging in a tropical environment?

    PubMed Central

    Weimerskirch, H.; Le Corre, M.; Ropert-Coudert, Y.; Kato, A.; Marsac, F.

    2005-01-01

    In seabirds a broad variety of morphologies, flight styles and feeding methods exist as an adaptation to optimal foraging in contrasted marine environments for a wide variety of prey types. Because of the low productivity of tropical waters it is expected that specific flight and foraging techniques have been selected there, but very few data are available. By using five different types of high-precision miniaturized logger (global positioning systems, accelerometers, time depth recorders, activity recorders, altimeters) we studied the way a seabird is foraging over tropical waters. Red-footed boobies are foraging in the day, never foraging at night, probably as a result of predation risks. They make extensive use of wind conditions, flying preferentially with crosswinds at median speed of 38 km h−1, reaching highest speeds with tail winds. They spent 66% of the foraging trip in flight, using a flap–glide flight, and gliding 68% of the flight. Travelling at low costs was regularly interrupted by extremely active foraging periods where birds are very frequently touching water for landing, plunge diving or surface diving (30 landings h−1). Dives were shallow (maximum 2.4 m) but frequent (4.5 dives h−1), most being plunge dives. While chasing for very mobile prey like flying fishes, boobies have adopted a very active and specific hunting behaviour, but the use of wind allows them to reduce travelling cost by their extensive use of gliding. During the foraging and travelling phases birds climb regularly to altitudes of 20–50 m to spot prey or congeners. During the final phase of the flight, they climb to high altitudes, up to 500 m, probably to avoid attacks by frigatebirds along the coasts. This study demonstrates the use by boobies of a series of very specific flight and activity patterns that have probably been selected as adaptations to the conditions of tropical waters. PMID:15875570

  15. Assessing Arboreal Adaptations of Bird Antecedents: Testing the Ecological Setting of the Origin of the Avian Flight Stroke

    PubMed Central

    Dececchi, T. Alexander; Larsson, Hans C. E.

    2011-01-01

    The origin of avian flight is a classic macroevolutionary transition with research spanning over a century. Two competing models explaining this locomotory transition have been discussed for decades: ground up versus trees down. Although it is impossible to directly test either of these theories, it is possible to test one of the requirements for the trees-down model, that of an arboreal paravian. We test for arboreality in non-avian theropods and early birds with comparisons to extant avian, mammalian, and reptilian scansors and climbers using a comprehensive set of morphological characters. Non-avian theropods, including the small, feathered deinonychosaurs, and Archaeopteryx, consistently and significantly cluster with fully terrestrial extant mammals and ground-based birds, such as ratites. Basal birds, more advanced than Archaeopteryx, cluster with extant perching ground-foraging birds. Evolutionary trends immediately prior to the origin of birds indicate skeletal adaptations opposite that expected for arboreal climbers. Results reject an arboreal capacity for the avian stem lineage, thus lending no support for the trees-down model. Support for a fully terrestrial ecology and origin of the avian flight stroke has broad implications for the origin of powered flight for this clade. A terrestrial origin for the avian flight stroke challenges the need for an intermediate gliding phase, presents the best resolved series of the evolution of vertebrate powered flight, and may differ fundamentally from the origin of bat and pterosaur flight, whose antecedents have been postulated to have been arboreal and gliding. PMID:21857918

  16. Assessing arboreal adaptations of bird antecedents: testing the ecological setting of the origin of the avian flight stroke.

    PubMed

    Dececchi, T Alexander; Larsson, Hans C E

    2011-01-01

    The origin of avian flight is a classic macroevolutionary transition with research spanning over a century. Two competing models explaining this locomotory transition have been discussed for decades: ground up versus trees down. Although it is impossible to directly test either of these theories, it is possible to test one of the requirements for the trees-down model, that of an arboreal paravian. We test for arboreality in non-avian theropods and early birds with comparisons to extant avian, mammalian, and reptilian scansors and climbers using a comprehensive set of morphological characters. Non-avian theropods, including the small, feathered deinonychosaurs, and Archaeopteryx, consistently and significantly cluster with fully terrestrial extant mammals and ground-based birds, such as ratites. Basal birds, more advanced than Archaeopteryx, cluster with extant perching ground-foraging birds. Evolutionary trends immediately prior to the origin of birds indicate skeletal adaptations opposite that expected for arboreal climbers. Results reject an arboreal capacity for the avian stem lineage, thus lending no support for the trees-down model. Support for a fully terrestrial ecology and origin of the avian flight stroke has broad implications for the origin of powered flight for this clade. A terrestrial origin for the avian flight stroke challenges the need for an intermediate gliding phase, presents the best resolved series of the evolution of vertebrate powered flight, and may differ fundamentally from the origin of bat and pterosaur flight, whose antecedents have been postulated to have been arboreal and gliding.

  17. Aerodynamic Flight-Test Results for the Adaptive Compliant Trailing Edge

    NASA Technical Reports Server (NTRS)

    Cumming, Stephen B.; Smith, Mark S.; Ali, Aliyah N.; Bui, Trong T.; Ellsworth, Joel C.; Garcia, Christian A.

    2016-01-01

    The aerodynamic effects of compliant flaps installed onto a modified Gulfstream III airplane were investigated. Analyses were performed prior to flight to predict the aerodynamic effects of the flap installation. Flight tests were conducted to gather both structural and aerodynamic data. The airplane was instrumented to collect vehicle aerodynamic data and wing pressure data. A leading-edge stagnation detection system was also installed. The data from these flights were analyzed and compared with predictions. The predictive tools compared well with flight data for small flap deflections, but differences between predictions and flight estimates were greater at larger deflections. This paper describes the methods used to examine the aerodynamics data from the flight tests and provides a discussion of the flight-test results in the areas of vehicle aerodynamics, wing sectional pressure coefficient profiles, and air data.

  18. Space physiology II: adaptation of the central nervous system to space flight--past, current, and future studies.

    PubMed

    Clément, Gilles; Ngo-Anh, Jennifer Thu

    2013-07-01

    Experiments performed in orbit on the central nervous system have focused on the control of posture, eye movements, spatial orientation, as well as cognitive processes, such as three-dimensional visual perception and mental representation of space. Brain activity has also been recorded during and immediately after space flight for evaluating the changes in brain structure activation during tasks involving perception, attention, memory, decision, and action. Recent ground-based studies brought evidence that the inputs from the neurovestibular system also participate in orthostatic intolerance. It is, therefore, important to revisit the flight data of neuroscience studies in the light of new models of integrative physiology. The outcomes of this exercise will increase our knowledge on the adaptation of body functions to changing gravitational environment, vestibular disorders, aging, and our approach towards more effective countermeasures during human space flight and planetary exploration.

  19. A review of adaptive change in musculoskeletal impedance during space flight and associated implications for postflight head movement control

    NASA Technical Reports Server (NTRS)

    McDonald, P. V.; Bloomberg, J. J.; Layne, C. S.

    1997-01-01

    We present a review of converging sources of evidence which suggest that the differences between loading histories experienced in 1-g and weightlessness are sufficient to stimulate adaptation in mechanical impedance of the musculoskeletal system. As a consequence of this adaptive change we argue that we should observe changes in the ability to attenuate force transmission through the musculoskeletal system both during and after space flight. By focusing attention on the relation between human sensorimotor activity and support surfaces, the importance of controlling mechanical energy flow through the musculoskeletal system is demonstrated. The implications of such control are discussed in light of visual-vestibular function in the specific context of head and gaze control during postflight locomotion. Evidence from locomotory biomechanics, visual-vestibular function, ergonomic evaluations of human vibration, and specific investigations of locomotion and head and gaze control after space flight, is considered.

  20. In-Flight Suppression of a Destabilized F/A-18 Structural Mode Using the Space Launch System Adaptive Augmenting Control System

    NASA Technical Reports Server (NTRS)

    Wall, John H.; VanZwieten, Tannen S.; Gilligan, Eric T.; Miller, Christopher J.; Hanson, Curtis E.; Orr, Jeb S.

    2015-01-01

    NASA's Space Launch System (SLS) Flight Control System (FCS) includes an Adaptive Augmenting Control (AAC) component which employs a multiplicative gain update law to enhance the performance and robustness of the baseline control system for extreme off nominal scenarios. The SLS FCS algorithm including AAC has been flight tested utilizing a specially outfitted F/A-18 fighter jet in which the pitch axis control of the aircraft was performed by a Non-linear Dynamic Inversion (NDI) controller, SLS reference models, and the SLS flight software prototype. This paper describes test cases from the research flight campaign in which the fundamental F/A-18 airframe structural mode was identified using frequency-domain reconstruction of flight data, amplified to result in closed loop instability, and suppressed in-flight by the SLS adaptive control system.

  1. In-Flight Suppression of an Unstable F/A-18 Structural Mode Using the Space Launch System Adaptive Augmenting Control System

    NASA Technical Reports Server (NTRS)

    VanZwieten, Tannen S.; Gilligan, Eric T.; Wall, John H.; Miller, Christopher J.; Hanson, Curtis E.; Orr, Jeb S.

    2015-01-01

    NASA's Space Launch System (SLS) Flight Control System (FCS) includes an Adaptive Augmenting Control (AAC) component which employs a multiplicative gain update law to enhance the performance and robustness of the baseline control system for extreme off-nominal scenarios. The SLS FCS algorithm including AAC has been flight tested utilizing a specially outfitted F/A-18 fighter jet in which the pitch axis control of the aircraft was performed by a Non-linear Dynamic Inversion (NDI) controller, SLS reference models, and the SLS flight software prototype. This paper describes test cases from the research flight campaign in which the fundamental F/A-18 airframe structural mode was identified using post-flight frequency-domain reconstruction, amplified to result in closed loop instability, and suppressed in-flight by the SLS adaptive control system.

  2. Impregnating magnetic components with MDA free epoxy

    SciTech Connect

    Sanchez, R.O.; Domeier, L.; Gunewardena, S.

    1995-08-01

    This paper describes the use of {open_quotes}Formula 456{close_quotes} an aliphatic amine cured epoxy for impregnating coils. Methylene dianiline (MDA) has been used for more than 20 years as the curing agent for various epoxy formulations throughout the Department of Energy. Sandia National Laboratories began the process of replacing MDA with other formulations because of regulations imposed by OSHA on the use of MDA.

  3. Adaptive Flight Control Design with Optimal Control Modification on an F-18 Aircraft Model

    NASA Technical Reports Server (NTRS)

    Burken, John J.; Nguyen, Nhan T.; Griffin, Brian J.

    2010-01-01

    In the presence of large uncertainties, a control system needs to be able to adapt rapidly to regain performance. Fast adaptation is referred to as the implementation of adaptive control with a large adaptive gain to reduce the tracking error rapidly; however, a large adaptive gain can lead to high-frequency oscillations which can adversely affect the robustness of an adaptive control law. A new adaptive control modification is presented that can achieve robust adaptation with a large adaptive gain without incurring high-frequency oscillations as with the standard model-reference adaptive control. The modification is based on the minimization of the Y2 norm of the tracking error, which is formulated as an optimal control problem. The optimality condition is used to derive the modification using the gradient method. The optimal control modification results in a stable adaptation and allows a large adaptive gain to be used for better tracking while providing sufficient robustness. A damping term (v) is added in the modification to increase damping as needed. Simulations were conducted on a damaged F-18 aircraft (McDonnell Douglas, now The Boeing Company, Chicago, Illinois) with both the standard baseline dynamic inversion controller and the adaptive optimal control modification technique. The results demonstrate the effectiveness of the proposed modification in tracking a reference model.

  4. Strain Gage Load Calibration of the Wing Interface Fittings for the Adaptive Compliant Trailing Edge Flap Flight Test

    NASA Technical Reports Server (NTRS)

    Miller, Eric J.; Holguin, Andrew C.; Cruz, Josue; Lokos, William A.

    2014-01-01

    This is the presentation to follow conference paper of the same name. The adaptive compliant trailing edge (ACTE) flap experiment safety of flight requires that the flap to wing interface loads be sensed and monitored in real time to ensure that the wing structural load limits are not exceeded. This paper discusses the strain gage load calibration testing and load equation derivation methodology for the ACTE interface fittings. Both the left and right wing flap interfaces will be monitored and each contains four uniquely designed and instrumented flap interface fittings. The interface hardware design and instrumentation layout are discussed. Twenty one applied test load cases were developed using the predicted in-flight loads for the ACTE experiment.

  5. Pilot Evaluation of Adaptive Control in Motion-Based Flight Simulator

    NASA Technical Reports Server (NTRS)

    Kaneshige, John T.; Campbell, Stefan Forrest

    2009-01-01

    The objective of this work is to assess the strengths, weaknesses, and robustness characteristics of several MRAC (Model-Reference Adaptive Control) based adaptive control technologies garnering interest from the community as a whole. To facilitate this, a control study using piloted and unpiloted simulations to evaluate sensitivities and handling qualities was conducted. The adaptive control technologies under consideration were ALR (Adaptive Loop Recovery), BLS (Bounded Linear Stability), Hybrid Adaptive Control, L1, OCM (Optimal Control Modification), PMRAC (Predictor-based MRAC), and traditional MRAC

  6. An adaptive dual-optimal path-planning technique for unmanned air vehicles with application to solar-regenerative high altitude long endurance flight

    NASA Astrophysics Data System (ADS)

    Whitfield, Clifford A.

    2009-12-01

    A multi-objective technique for Unmanned Air Vehicle (UAV) path and trajectory autonomy generation, through task allocation and sensor fusion has been developed. The Dual-Optimal Path-Planning (D-O.P-P.) Technique generates on-line adaptive flight paths for UAVs based on available flight windows and environmental influenced objectives. The environmental influenced optimal condition, known as the driver' determines the condition, within a downstream virtual window of possible vehicle destinations and orientation built from the UAV kinematics. The intermittent results are pursued by a dynamic optimization technique to determine the flight path. This sequential optimization technique is a multi-objective optimization procedure consisting of two goals, without requiring additional information to combine the conflicting objectives into a single-objective. An example case-study and additional applications are developed and the results are discussed; including the application to the field of Solar Regenerative (SR) High Altitude Long Endurance (HALE) UAV flight. Harnessing solar energy has recently been adapted for use on high altitude UAV platforms. An aircraft that uses solar panels and powered by the sun during the day and through the night by SR systems, in principle could sustain flight for weeks or months. The requirements and limitations of solar powered flight were determined. The SR-HALE UAV platform geometry and flight characteristics were selected from an existing aircraft that has demonstrated the capability for sustained flight through flight tests. The goals were to maintain continual Situational Awareness (SA) over a case-study selected Area of Interest (AOI) and existing UAV power and surveillance systems. This was done for still wind and constant wind conditions at altitude along with variations in latitude. The characteristics of solar flux and the dependence on the surface location and orientation were established along with fixed flight maneuvers for

  7. Evolution by flight and fight: diverse mechanisms of adaptation by actively motile microbes.

    PubMed

    Rendueles, Olaya; Velicer, Gregory J

    2017-02-01

    Evolutionary adaptation can be achieved by mechanisms accessible to all organisms, including faster growth and interference competition, but self-generated motility offers additional possibilities. We tested whether 55 populations of the bacterium Myxococcus xanthus that underwent selection for increased fitness at the leading edge of swarming colonies adapted by swarming faster toward unused resources or by other means. Populations adapted greatly but diversified markedly in both swarming phenotypes and apparent mechanisms of adaptation. Intriguingly, although many adapted populations swarm intrinsically faster than their ancestors, numerous others do not. Some populations evolved interference competition toward their ancestors, whereas others gained the ability to facultatively increase swarming rate specifically upon direct interaction with ancestral competitors. Our results both highlight the diverse range of mechanisms by which actively motile organisms can adapt evolutionarily and help to explain the high levels of swarming-phenotype diversity found in local soil populations of M. xanthus.

  8. Evolution by flight and fight: diverse mechanisms of adaptation by actively motile microbes

    PubMed Central

    Rendueles, Olaya; Velicer, Gregory J

    2017-01-01

    Evolutionary adaptation can be achieved by mechanisms accessible to all organisms, including faster growth and interference competition, but self-generated motility offers additional possibilities. We tested whether 55 populations of the bacterium Myxococcus xanthus that underwent selection for increased fitness at the leading edge of swarming colonies adapted by swarming faster toward unused resources or by other means. Populations adapted greatly but diversified markedly in both swarming phenotypes and apparent mechanisms of adaptation. Intriguingly, although many adapted populations swarm intrinsically faster than their ancestors, numerous others do not. Some populations evolved interference competition toward their ancestors, whereas others gained the ability to facultatively increase swarming rate specifically upon direct interaction with ancestral competitors. Our results both highlight the diverse range of mechanisms by which actively motile organisms can adapt evolutionarily and help to explain the high levels of swarming-phenotype diversity found in local soil populations of M. xanthus. PMID:27662568

  9. Development of Micro Air Vehicle Technology With In-Flight Adaptive-Wing Structure

    NASA Technical Reports Server (NTRS)

    Waszak, Martin R. (Technical Monitor); Shkarayev, Sergey; Null, William; Wagner, Matthew

    2004-01-01

    This is a final report on the research studies, "Development of Micro Air Vehicle Technology with In-Flight Adaptrive-Wing Structure". This project involved the development of variable-camber technology to achieve efficient design of micro air vehicles. Specifically, it focused on the following topics: 1) Low Reynolds number wind tunnel testing of cambered-plate wings. 2) Theoretical performance analysis of micro air vehicles. 3) Design of a variable-camber MAV actuated by micro servos. 4) Test flights of a variable-camber MAV.

  10. L(sub 1) Adaptive Control Design for NASA AirSTAR Flight Test Vehicle

    NASA Technical Reports Server (NTRS)

    Gregory, Irene M.; Cao, Chengyu; Hovakimyan, Naira; Zou, Xiaotian

    2009-01-01

    In this paper we present a new L(sub 1) adaptive control architecture that directly compensates for matched as well as unmatched system uncertainty. To evaluate the L(sub 1) adaptive controller, we take advantage of the flexible research environment with rapid prototyping and testing of control laws in the Airborne Subscale Transport Aircraft Research system at the NASA Langley Research Center. We apply the L(sub 1) adaptive control laws to the subscale turbine powered Generic Transport Model. The presented results are from a full nonlinear simulation of the Generic Transport Model and some preliminary pilot evaluations of the L(sub 1) adaptive control law.

  11. Request for Information Response for the Flight Validation of Adaptive Control to Prevent Loss-of-Control Events. Overview of RFI Responses

    NASA Technical Reports Server (NTRS)

    Bosworth, John T.

    2009-01-01

    Adaptive control should be integrated with a baseline controller and only used when necessary (5 responses). Implementation as an emergency system. Immediately re-stabilize and return to controlled flight. Forced perturbation (excitation) for fine-tuning system a) Check margins; b) Develop requirements for amplitude of excitation. Adaptive system can improve performance by eating into margin constraints imposed on the non-adaptive system. Nonlinear effects due to multi-string voting.

  12. Spatial perception changes associated with space flight: implications for adaptation to altered inertial environments.

    PubMed

    Parker, Donald E

    2003-01-01

    Preparation for extended travel by astronauts within the Solar System, including a possible manned mission to Mars, requires more complete understanding of adaptation to altered inertial environments. Improved understanding is needed to support development and evaluation of interventions to facilitate adaptations during transitions between those environments. Travel to another planet escalates the adaptive challenge because astronauts will experience prolonged exposure to microgravity before encountering a novel gravitational environment. This challenge would have to be met without ground support at the landing site. Evaluation of current adaptive status as well as intervention efficacy can be performed using perceptual, eye movement and postural measures. Due to discrepancies of adaptation magnitude and time-course among these measures, complete understanding of adaptation processes, as well as intervention evaluation, requires examination of all three. Previous research and theory that provide models for comprehending adaptation to altered inertial environments are briefly examined. Reports from astronauts of selected pre- in- and postflight self-motion illusions are described. The currently controversial tilt-translation reinterpretation hypothesis is reviewed and possible resolutions to the controversy are proposed. Finally, based on apparent gaps in our current knowledge, further research is proposed to achieve a more complete understanding of adaptation as well as to develop effective counter-measures.

  13. Falcon versus grouse: flight adaptations of a predator and its prey

    USGS Publications Warehouse

    Pennycuick, C.J.; Fuller, M.R.; Oar, J.J.; Kirkpatrick, S.J.

    1994-01-01

    Several falcons were trained to fly along a 500 m course to a lure. The air speeds of the more consistent performers averaged about 1.5 times their calculated minimum power speeds, and occasionally reached 2.1 times the minimum power speed. Wing beat frequencies of all the falcons were above those estimated from earlier field observations, and the same was true of wild Sage Grouse Centrocercus urophasianus, a regular falconer's quarry in the study area. Measurements of grouse killed by falcons showed that their wings were short, with broad slotted tips, whereas the falcons' wings were longer in relation to their body mass, and tapered. The short wings of grouse result in fast flight, high power requirements, and reduced capacity for aerobic flight. Calculations indicated that the grouse should fly faster than the falcons, and had the large amount of flight muscle needed to do so, but that the falcons would be capable of prolonged aerobic flight, whereas the grouse probably would not. We surmise that Sage Grouse cannot fly continuously without incurring an oxygen debt, and are therefore not long-distance migrants, although this limitation is partly due to their large size, and would not apply to smaller galliform birds such as ptarmigan Lagopus spp. The wing action seen in video recordings of the falcons was not consistent with the maintenance of constant circulation. We call it 'chase mode' because it appears to be associated with a high level of muscular exertion, without special regard to fuel economy. It shows features in common with the 'bounding' flight of passerines.

  14. Adapt

    NASA Astrophysics Data System (ADS)

    Bargatze, L. F.

    2015-12-01

    Active Data Archive Product Tracking (ADAPT) is a collection of software routines that permits one to generate XML metadata files to describe and register data products in support of the NASA Heliophysics Virtual Observatory VxO effort. ADAPT is also a philosophy. The ADAPT concept is to use any and all available metadata associated with scientific data to produce XML metadata descriptions in a consistent, uniform, and organized fashion to provide blanket access to the full complement of data stored on a targeted data server. In this poster, we present an application of ADAPT to describe all of the data products that are stored by using the Common Data File (CDF) format served out by the CDAWEB and SPDF data servers hosted at the NASA Goddard Space Flight Center. These data servers are the primary repositories for NASA Heliophysics data. For this purpose, the ADAPT routines have been used to generate data resource descriptions by using an XML schema named Space Physics Archive, Search, and Extract (SPASE). SPASE is the designated standard for documenting Heliophysics data products, as adopted by the Heliophysics Data and Model Consortium. The set of SPASE XML resource descriptions produced by ADAPT includes high-level descriptions of numerical data products, display data products, or catalogs and also includes low-level "Granule" descriptions. A SPASE Granule is effectively a universal access metadata resource; a Granule associates an individual data file (e.g. a CDF file) with a "parent" high-level data resource description, assigns a resource identifier to the file, and lists the corresponding assess URL(s). The CDAWEB and SPDF file systems were queried to provide the input required by the ADAPT software to create an initial set of SPASE metadata resource descriptions. Then, the CDAWEB and SPDF data repositories were queried subsequently on a nightly basis and the CDF file lists were checked for any changes such as the occurrence of new, modified, or deleted

  15. Investigation of the Multiple Method Adaptive Control (MMAC) method for flight control systems

    NASA Technical Reports Server (NTRS)

    Athans, M.; Baram, Y.; Castanon, D.; Dunn, K. P.; Green, C. S.; Lee, W. H.; Sandell, N. R., Jr.; Willsky, A. S.

    1979-01-01

    The stochastic adaptive control of the NASA F-8C digital-fly-by-wire aircraft using the multiple model adaptive control (MMAC) method is presented. The selection of the performance criteria for the lateral and the longitudinal dynamics, the design of the Kalman filters for different operating conditions, the identification algorithm associated with the MMAC method, the control system design, and simulation results obtained using the real time simulator of the F-8 aircraft at the NASA Langley Research Center are discussed.

  16. (A new time of flight) Acoustic flow meter using wide band signals and adaptive beamforming techniques

    NASA Astrophysics Data System (ADS)

    Murgan, I.; Ioana, C.; Candel, I.; Anghel, A.; Ballester, J. L.; Reeb, B.; Combes, G.

    2016-11-01

    In this paper we present the result of our research concerning the improvement of acoustic time of flight flow metering for water pipes. Current flow meters are based on the estimation of direct time of flight by matched filtering of the received and emitted signals by acoustic transducers. Currently, narrow band signals are used, as well as a single emitter/receptor transducer configuration. Although simple, this configuration presents a series of limitations such as energy losses due to pipe wall/water interface, pressure/flow transients, sensitivity to flow induced vibrations, acoustic beam deformations and shift due to changes in flow velocity and embedded turbulence in the flow. The errors associated with these limitations reduce the overall robustness of existing flow meters, as well as the measured flow rate range and lower accuracy. In order to overcome these limitations, two major innovations were implemented at the signal processing level. The first one concerns the use of wide band signals that optimise the power transfer throughout the acoustic path and also increase the number of velocity/flow readings per second. Using wide band signals having a high duration-bandwidth product increases the precision in terms of time of flight measurements and, in the same time, improves the system robustness. The second contribution consists in the use of a multiple emitter - multiple receivers configuration (for one path) in order to compensate the emitted acoustic beam shift, compensate the time of flight estimation errors and thus increase the flow meter's robustness in case of undesired effects such as the “flow blow” and transient/rapid flow rate/velocity changes. Using a new signal processing algorithm that take advantage of the controlled wide band content coming from multiple receivers, the new flow meters achieves a higher accuracy in terms of flow velocity over a wider velocity range than existing systems. Tests carried out on real scale experimental

  17. [The features of adaptation and disadaptation of the human cardiovascular system in the space flight conditions].

    PubMed

    Kotovskaia, A R; Fomina, G A

    2010-01-01

    The work was aimed at analysis and generalization of the hemodynamic data collected over 20 years from 26 cosmonauts flown 8 to 438 days aboard orbital stations Salyut 7 and Mir. The paper presents the results of ultrasonic investigations of the heart, arterial and venous peripheral vessels in different parts of human body, and measurements of leg veins capacity with the use of occlusive plethysmograpy. It was shown that in the resting condition such prime hemodynamic parameters as the pumping function of the heart and blood supply of the brain, and integral parameters, i.e. arterial pressure and heat rate, were best "protected" as they demonstrated stability throughout long exposure in microgravity. In the absence of gravitational stimulation, arterial resistance went down in essentially all vascular regions below the heart level; to put it differently, the anti-gravity distribution of the vascular tone was annulled gradually as unneeded in microgravity. Compared with the data about arteries, venous hemodynamics was found to be particularly sensitive considering the early advent and significance of changes. Venous return slowed down, resistance of the lower body vessels declined and capacity of the leg venous net increased. Functional testing with the lower body negative pressure revealed degradation of the gravity-dependent reactions that became more conspicuous as flight duration extended further. Cardiovascular deconditioning showed itself clearly on return to Earth's gravity by decreased g-tolerance during re-entry and orthostatic instability post flight. These investigations provided objective evidence for multifactorial genesis of orthostatic instability during space flight including blood redistribution, altered tone regulation of leg's venous and arterial vessels and hypovolemia.

  18. In-Flight Suppression of a De-Stabilized F/A-18 Structural Mode Using the Space Launch System Adaptive Augmenting Control System

    NASA Technical Reports Server (NTRS)

    Wall, John; VanZwieten, Tannen; Giiligan Eric; Miller, Chris; Hanson, Curtis; Orr, Jeb

    2015-01-01

    Adaptive Augmenting Control (AAC) has been developed for NASA's Space Launch System (SLS) family of launch vehicles and implemented as a baseline part of its flight control system (FCS). To raise the technical readiness level of the SLS AAC algorithm, the Launch Vehicle Adaptive Control (LVAC) flight test program was conducted in which the SLS FCS prototype software was employed to control the pitch axis of Dryden's specially outfitted F/A-18, the Full Scale Advanced Systems Test Bed (FAST). This presentation focuses on a set of special test cases which demonstrate the successful mitigation of the unstable coupling of an F/A-18 airframe structural mode with the SLS FCS.

  19. The Development Of Drosophila Melanogaster under Different Duration Space Flight and Subsequent Adaptation to Earth Gravity.

    PubMed

    Ogneva, Irina V; Belyakin, Stepan N; Sarantseva, Svetlana V

    2016-01-01

    In prospective human exploration of outer space, the need to preserve a species over several generations under changed gravity conditions may arise. This paper demonstrates our results in the creation of the third generation of fruit fly Drosophila melanogaster (third-stage larvae) during the 44.5-day space flight (Foton-M4 satellite (2014, Russia)), then the fourth generation on Earth and the fifth generation again in conditions of the 12-day space flight (2014, in the Russian Segment of the ISS). The species preserves fertility despite a number of changes in the level of expression and content of cytoskeletal proteins, which are the key components of the cleavage spindle and the contractile ring of cells. The results of transcriptome screening and space analysis of cytoskeletal proteins show that the exposure to weightless conditions leads to the increased transcription of metabolic genes, cuticle components and the decreased transcription of genes involved in morphogenesis, cell differentiation, cytoskeletal organization and genes associated with the plasma membrane. "Subsequent" exposure to the microgravity for 12 days resulted in an even more significant increase/decrease in the transcription of the same genes. On the contrary, the transition from the microgravity conditions to the gravity of Earth leads to the increased transcription of genes whose products are involved in the morphogenesis, cytoskeletal organization, motility of cells and transcription regulation, and to the decreased transcription of cuticle genes and proteolytic processes.

  20. The Development Of Drosophila Melanogaster under Different Duration Space Flight and Subsequent Adaptation to Earth Gravity

    PubMed Central

    Belyakin, Stepan N.; Sarantseva, Svetlana V.

    2016-01-01

    In prospective human exploration of outer space, the need to preserve a species over several generations under changed gravity conditions may arise. This paper demonstrates our results in the creation of the third generation of fruit fly Drosophila melanogaster (third-stage larvae) during the 44.5-day space flight (Foton-M4 satellite (2014, Russia)), then the fourth generation on Earth and the fifth generation again in conditions of the 12-day space flight (2014, in the Russian Segment of the ISS). The species preserves fertility despite a number of changes in the level of expression and content of cytoskeletal proteins, which are the key components of the cleavage spindle and the contractile ring of cells. The results of transcriptome screening and space analysis of cytoskeletal proteins show that the exposure to weightless conditions leads to the increased transcription of metabolic genes, cuticle components and the decreased transcription of genes involved in morphogenesis, cell differentiation, cytoskeletal organization and genes associated with the plasma membrane. “Subsequent” exposure to the microgravity for 12 days resulted in an even more significant increase/decrease in the transcription of the same genes. On the contrary, the transition from the microgravity conditions to the gravity of Earth leads to the increased transcription of genes whose products are involved in the morphogenesis, cytoskeletal organization, motility of cells and transcription regulation, and to the decreased transcription of cuticle genes and proteolytic processes. PMID:27861601

  1. Multivariate Dynamic Modeling to Investigate Human Adaptation to Space Flight: Initial Concepts

    NASA Technical Reports Server (NTRS)

    Shelhamer, Mark; Mindock, Jennifer; Zeffiro, Tom; Krakauer, David; Paloski, William H.; Lumpkins, Sarah

    2014-01-01

    The array of physiological changes that occur when humans venture into space for long periods presents a challenge to future exploration. The changes are conventionally investigated independently, but a complete understanding of adaptation requires a conceptual basis founded in integrative physiology, aided by appropriate mathematical modeling. NASA is in the early stages of developing such an approach.

  2. Multivariate Dynamical Modeling to Investigate Human Adaptation to Space Flight: Initial Concepts

    NASA Technical Reports Server (NTRS)

    Shelhamer, Mark; Mindock, Jennifer; Zeffiro, Tom; Krakauer, David; Paloski, William H.; Lumpkins, Sarah

    2014-01-01

    The array of physiological changes that occur when humans venture into space for long periods presents a challenge to future exploration. The changes are conventionally investigated independently, but a complete understanding of adaptation requires a conceptual basis founded in intergrative physiology, aided by appropriate mathematical modeling. NASA is in the early stages of developing such an approach.

  3. Elastic Proteins in the Flight Muscle of Manduca sexta

    PubMed Central

    Yuan, Chen-Ching; Ma, Weikang; Schemmel, Peter; Cheng, Yu-Shu; Liu, Jiangmin; Tsaprailis, George; Feldman, Samuel; Southgate, Agnes Ayme; Irving, Thomas C.

    2015-01-01

    The flight muscles (DLM1) of the Hawkmoth, Manduca sexta are synchronous, requiring a neural spike for each contraction. Stress/strain curves of skinned DLM1 showed hysteresis indicating the presence of titin-like elastic proteins. Projectin and kettin are titin-like proteins previously identified in Lethocerus and Drosophila flight muscles. Analysis of Manduca muscles with 1% SDS-agarose gels and western blots showed two bands near 1 MDa that cross-reacted with antibodies to Drosophila projectin. Antibodies to Drosophila kettin cross-reacted to bands at ~500 and ~700 kDa, but also to bands at ~1.6 and ~2.1 MDa, that had not been previously observed in insect flight muscles. Mass spectrometry identified the 2.1 MDa protein as a product of the Sallimus (sls) gene. Analysis of the gene sequence showed that all 4 putative Sallimus and kettin isoforms could be explained as products of alternative splicing of the single sls gene. Both projectin and Sallimus isoforms were expressed to higher levels in ventrally located DLM1 subunits, primarily responsible for active work production, as compared to dorsally located subunits, which may act as damped springs. The different expression levels of the 2 projectin isoforms and 4 Sallimus/kettin isoforms may be adaptations to the specific requirements of individual muscle subunits. PMID:25602701

  4. Adaptive Responses in Eye-Head-Hand Coordination Following Exposures to a Virtual Environment as a Possible Space Flight Analog

    NASA Technical Reports Server (NTRS)

    Harm, Deborah L.; Taylor, L. C.; Bloomberg, J. J.

    2007-01-01

    Virtual environments (VE) offer unique training opportunities, particularly for training astronauts and preadapting them to the novel sensory conditions of microgravity. Sensorimotor aftereffects of VEs are often quite similar to adaptive sensorimotor responses observed in astronauts during and/or following space flight. The purpose of this research was to compare disturbances in sensorimotor coordination produced by dome virtual environment display and to examine the effects of exposure duration, and repeated exposures to VR systems. The current study examined disturbances in eye-head-hand (EHH) and eye-head coordination. Preliminary results will be presented. Eleven subjects have participated in the study to date. One training session was completed in order to achieve stable performance on the EHH coordination and VE tasks. Three experimental sessions were performed each separated by one day. Subjects performed a navigation and pick and place task in a dome immersive display VE for 30 or 60 min. The subjects were asked to move objects from one set of 15 pedestals to the other set across a virtual square room through a random pathway as quickly and accurately as possible. EHH coordination was measured before, immediately after, and at 1 hr, 2 hr, 4 hr and 6 hr following exposure to VR. EHH coordination was measured as position errors and reaction time in a pointing task that included multiple horizontal and vertical LED targets. Repeated measures ANOVAs were used to analyze the data. In general, we observed significant increases in position errors for both horizontal and vertical targets. The largest decrements were observed immediately following exposure to VR and showed a fairly rapid recovery across test sessions, but not across days. Subjects generally showed faster RTs across days. Individuals recovered from the detrimental effects of exposure to the VE on position errors within 1-2 hours. The fact that subjects did not significantly improve across days

  5. Work, exercise, and space flight. 2: Modification of adaptation by exercise (exercise prescription)

    NASA Technical Reports Server (NTRS)

    Thornton, William

    1989-01-01

    The fundamentals of exercise theory on earth must be rigorously understood and applied to prevent adaptation to long periods of weightlessness. Locomotor activity, not weight, determines the capacity or condition of the largest muscles and bones in the body and usually also determines cardio-respiratory capacity. Absence of this activity results in rapid atrophy of muscle, bone, and cardio-respiratory capacity. Upper body muscle and bone are less affected depending upon the individual's usual, or 1-g, activities. Methodology is available to prevent these changes but space operations demand that it be done in the most efficient fashion, i.e., shortest time. At this point in time we can reasonably select the type of exercise and methods of obtaining it, but additional work in 1-g will be required to optimize the time.

  6. An implementable digital adaptive flight controller designed using stabilized single-stage algorithms

    NASA Technical Reports Server (NTRS)

    Alag, G.; Kaufman, H.

    1977-01-01

    An explicit adaptive controller, which makes direct use of on-line parameter identification, has been developed and applied to both the linearized and nonlinear equations of motion for the F-8 aircraft. This controller is composed of an on-line weighted least squares parameter identifier, a Kalman state filter, and a real model following control law designed using single-stage performance indices. The corresponding control gains are readily adjustable in accordance with parameter changes to ensure asymptotic stability if the conditions of perfect model following are satisfied, and stability in the sense of boundedness otherwise. Simulation experiments with realistic measurement noise indicate that the controller was effective in compensating for parameter variations and capable of rapid recovery from a set of erroneous initial parameter estimates which defined a set of destabilizing gains.

  7. Stability Metrics for Simulation and Flight-Software Assessment and Monitoring of Adaptive Control Assist Compensators

    NASA Technical Reports Server (NTRS)

    Hodel, A. S.; Whorton, Mark; Zhu, J. Jim

    2008-01-01

    Due to a need for improved reliability and performance in aerospace systems, there is increased interest in the use of adaptive control or other nonlinear, time-varying control designs in aerospace vehicles. While such techniques are built on Lyapunov stability theory, they lack an accompanying set of metrics for the assessment of stability margins such as the classical gain and phase margins used in linear time-invariant systems. Such metrics must both be physically meaningful and permit the user to draw conclusions in a straightforward fashion. We present in this paper a roadmap to the development of metrics appropriate to nonlinear, time-varying systems. We also present two case studies in which frozen-time gain and phase margins incorrectly predict stability or instability. We then present a multi-resolution analysis approach that permits on-line real-time stability assessment of nonlinear systems.

  8. Strain Gage Load Calibration of the Wing Interface Fittings for the Adaptive Compliant Trailing Edge Flap Flight Test

    NASA Technical Reports Server (NTRS)

    Miller, Eric J.; Holguin, Andrew C.; Cruz, Josue; Lokos, William A.

    2014-01-01

    The safety-of-flight parameters for the Adaptive Compliant Trailing Edge (ACTE) flap experiment require that flap-to-wing interface loads be sensed and monitored in real time to ensure that the structural load limits of the wing are not exceeded. This paper discusses the strain gage load calibration testing and load equation derivation methodology for the ACTE interface fittings. Both the left and right wing flap interfaces were monitored; each contained four uniquely designed and instrumented flap interface fittings. The interface hardware design and instrumentation layout are discussed. Twenty-one applied test load cases were developed using the predicted in-flight loads. Pre-test predictions of strain gage responses were produced using finite element method models of the interface fittings. Predicted and measured test strains are presented. A load testing rig and three hydraulic jacks were used to apply combinations of shear, bending, and axial loads to the interface fittings. Hardware deflections under load were measured using photogrammetry and transducers. Due to deflections in the interface fitting hardware and test rig, finite element model techniques were used to calculate the reaction loads throughout the applied load range, taking into account the elastically-deformed geometry. The primary load equations were selected based on multiple calibration metrics. An independent set of validation cases was used to validate each derived equation. The 2-sigma residual errors for the shear loads were less than eight percent of the full-scale calibration load; the 2-sigma residual errors for the bending moment loads were less than three percent of the full-scale calibration load. The derived load equations for shear, bending, and axial loads are presented, with the calculated errors for both the calibration cases and the independent validation load cases.

  9. Loss of RIG-I leads to a functional replacement with MDA5 in the Chinese tree shrew

    PubMed Central

    Xu, Ling; Yu, Dandan; Fan, Yu; Peng, Li; Wu, Yong; Yao, Yong-Gang

    2016-01-01

    The function of the RIG-I-like receptors (RLRs; including RIG-I, MDA5, and LGP2) as key cytoplasmic sensors of viral pathogen-associated molecular patterns (PAMPs) has been subjected to numerous pathogenic challenges and has undergone a dynamic evolution. We found evolutionary evidence that RIG-I was lost in the Chinese tree shrew lineage. Along with the loss of RIG-I, both MDA5 (tMDA5) and LGP2 (tLGP2) have undergone strong positive selection in the tree shrew. tMDA5 or tMDA5/tLGP2 could sense Sendai virus (an RNA virus posed as a RIG-I agonist) for inducing type I IFN, although conventional RIG-I and MDA5 were thought to recognize distinct RNA structures and viruses. tMDA5 interacted with adaptor tMITA (STINGTMEM173/ERIS), which was reported to bind only with RIG-I. The positively selected sites in tMDA5 endowed the substitute function for the lost RIG-I. These findings provided insights into the adaptation and functional diversity of innate antiviral activity in vertebrates. PMID:27621475

  10. The Skylab Airlock Module and Multiple Docking Adapter

    NASA Technical Reports Server (NTRS)

    1972-01-01

    This photograph shows the flight article of the mated Airlock Module (AM) and Multiple Docking Adapter (MDA) being lowering into horizontal position on a transporter. Although the AM and the MDA were separate entities, they were in many respects simply two components of a single module. The AM enabled crew members to conduct extravehicular activities outside Skylab as required for experiment support. Oxygen and nitrogen storage tanks needed for Skylab's life support system were mounted on the external truss work of the AM. Major components in the AM included Skylab's electric power control and distribution station, environmental control system, communication system, and data handling and recording systems. The MDA, forward of the AM, provided docking facilities for the Command and Service Module. It also accommodated several experiment systems, among them the Earth Resource Experiment Package, the materials processing facility, and the control and display console needed for the Apollo Telescope Mount solar astronomy studies. The AM was built by McDornell Douglas and the MDA was built by Martin Marietta. The Marshall Space Flight Center was responsible for the design and development of the Skylab hardware and experiment management.

  11. The Skylab Airlock Module and Multiple Docking Adapter

    NASA Technical Reports Server (NTRS)

    1972-01-01

    This photograph shows the flight article of the Airlock Module (AM)/Multiple Docking Adapter (MDA) assembly being readied for testing in a clean room at the McDornell Douglas Plant in St. Louis, Missouri. Although the AM and the MDA were separate entities, they were in many respects simply two components of a single module. The AM enabled crew members to conduct extravehicular activities outside Skylab as required for experiment support. Oxygen and nitrogen storage tanks needed for Skylab's life support system were mounted on the external truss work of the AM. Major components in the AM included Skylab's electric power control and distribution station, environmental control system, communication system, and data handling and recording systems. The MDA, forward of the AM, provided docking facilities for the Command and Service Module. It also accommodated several experiment systems, among them the Earth Resource Experiment Package, the materials processing facility, and the control and display console needed for the Apollo Telescope Mount solar astronomy studies. The AM was built by McDonnell Douglas and the MDA was built by Martin Marietta. The Marshall Space Flight Center was responsible for the design and development of the Skylab hardware and experiment management.

  12. Longitudinal Aerodynamic Modeling of the Adaptive Compliant Trailing Edge Flaps on a GIII Airplane and Comparisons to Flight Data

    NASA Technical Reports Server (NTRS)

    Smith, Mark S.; Bui, Trong T.; Garcia, Christian A.; Cumming, Stephen B.

    2016-01-01

    A pair of compliant trailing edge flaps was flown on a modified GIII airplane. Prior to flight test, multiple analysis tools of various levels of complexity were used to predict the aerodynamic effects of the flaps. Vortex lattice, full potential flow, and full Navier-Stokes aerodynamic analysis software programs were used for prediction, in addition to another program that used empirical data. After the flight-test series, lift and pitching moment coefficient increments due to the flaps were estimated from flight data and compared to the results of the predictive tools. The predicted lift increments matched flight data well for all predictive tools for small flap deflections. All tools over-predicted lift increments for large flap deflections. The potential flow and Navier-Stokes programs predicted pitching moment coefficient increments better than the other tools.

  13. Solid film lubricants and thermal control coatings flown aboard the EOIM-3 MDA sub-experiment

    NASA Technical Reports Server (NTRS)

    Murphy, Taylor J.; David, Kaia E.; Babel, Hank W.

    1995-01-01

    Additional experimental data were desired to support the selection of candidate thermal control coatings and solid film lubricants for the McDonnell Douglas Aerospace (MDA) Space Station hardware. The third Evaluation of Oxygen Interactions With Materials Mission (EOIM-3) flight experiment presented an opportunity to study the effects of the low Earth orbit environment on thermal control coatings and solid film lubricants. MDA provided five solid film lubricants and two anodic thermal control coatings for EOIM-3. The lubricant sample set consisted of three solid film lubricants with organic binders one solid film lubricant with an inorganic binder, and one solid film lubricant with no binder. The anodize coating sample set consisted of undyed sulfuric acid anodize and cobalt sulfide dyed sulfuric acid anodize, each on two different substrate aluminum alloys. The organic and inorganic binders in the solid film lubricants experienced erosion, and the lubricating pigments experienced oxidation. MDA is continuing to assess the effect of exposure to the low Earth orbit environment on the life and friction properties of the lubricants. Results to date support the design practice of shielding solid film lubricants from the low Earth orbit environment. Post-flight optical property analysis of the anodized specimens indicated that there were limited contamination effects and some atomic oxygen and ultraviolet radiation effects. These effects appeared to be within the values predicted by simulated ground testing and analysis of these materials, and they were different for each coating and substrate.

  14. Regulation of mda-7 gene expression during human melanoma differentiation.

    PubMed

    Madireddi, M T; Dent, P; Fisher, P B

    2000-03-02

    Induction of irreversible growth arrest and terminal differentiation in human melanoma cells following treatment with recombinant human fibroblast interferon (IFN-beta) and mezerein (MEZ) results in elevated expression of a specific melanoma differentiation associated gene, mda-7. Experiments were conducted to define the mechanism involved in the regulation of mda-7 expression in differentiating human melanoma cells. The mda-7 gene is actively transcribed in uninduced HO-1 human melanoma cells and the rate of transcription of mda-7 is not significantly enhanced by treatment with IFN-beta, MEZ or IFN-beta+MEZ. The high basal activity of the mda-7 promoter in uninduced melanoma cells and the absence of enhancing effect upon treatment with differentiation inducers is corroborated by transfection studies using the promoter region of mda-7 linked to a luciferase reporter gene containing the SV40 polyadenylation signal sequence. RT - PCR analysis detects the presence of low levels of mda-7 transcripts in uninduced and concomitant increases in differentiation inducer treated HO-1 cells. However, steady-state mda-7 mRNA is detected only in IFN-beta+MEZ and to a lesser degree in MEZ treated cells. We show that induction of terminal differentiation of HO-1 cells with IFN-beta+MEZ dramatically increases the half-life of mda-7 mRNA while treatment with cycloheximide results in detectable mda-7 mRNA in control and inducer treated cells. These observations confirm constitutive activity of the mda-7 promoter in HO-1 cells irrespective of differentiation status suggesting posttranscriptional processes as important determinants of mda-7 expression during terminal differentiation. The 3' UTR region of mda-7 contains AU-rich elements (ARE) that contribute to rapid mda-7 mRNA turnover during proliferation and reversible differentiation, a process controlled by a labile protein factor(s). Substitution of the SV40 polyadenylation signal sequence in the luciferase reporter plasmid with

  15. Adaptation.

    PubMed

    Broom, Donald M

    2006-01-01

    The term adaptation is used in biology in three different ways. It may refer to changes which occur at the cell and organ level, or at the individual level, or at the level of gene action and evolutionary processes. Adaptation by cells, especially nerve cells helps in: communication within the body, the distinguishing of stimuli, the avoidance of overload and the conservation of energy. The time course and complexity of these mechanisms varies. Adaptive characters of organisms, including adaptive behaviours, increase fitness so this adaptation is evolutionary. The major part of this paper concerns adaptation by individuals and its relationships to welfare. In complex animals, feed forward control is widely used. Individuals predict problems and adapt by acting before the environmental effect is substantial. Much of adaptation involves brain control and animals have a set of needs, located in the brain and acting largely via motivational mechanisms, to regulate life. Needs may be for resources but are also for actions and stimuli which are part of the mechanism which has evolved to obtain the resources. Hence pigs do not just need food but need to be able to carry out actions like rooting in earth or manipulating materials which are part of foraging behaviour. The welfare of an individual is its state as regards its attempts to cope with its environment. This state includes various adaptive mechanisms including feelings and those which cope with disease. The part of welfare which is concerned with coping with pathology is health. Disease, which implies some significant effect of pathology, always results in poor welfare. Welfare varies over a range from very good, when adaptation is effective and there are feelings of pleasure or contentment, to very poor. A key point concerning the concept of individual adaptation in relation to welfare is that welfare may be good or poor while adaptation is occurring. Some adaptation is very easy and energetically cheap and

  16. 40 CFR 721.10393 - Sodium bromide MDA complex (generic).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 31 2014-07-01 2014-07-01 false Sodium bromide MDA complex (generic... Specific Chemical Substances § 721.10393 Sodium bromide MDA complex (generic). (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified generically as sodium...

  17. Behavioral Health and Performance Operations at the NASA Johnson Space Center: A Comprehensive Program that Addresses Flight and Spaceflight Duty Adaptability

    NASA Technical Reports Server (NTRS)

    Beven, G. E.

    2017-01-01

    NASA astronauts on active status require medical certification for aircraft flying duties as well as readiness for long duration spaceflight training, launch to the International Space Station (ISS), and mission continuation during spaceflight operations. Behavioral fitness and adaptability is an inherent component of medical certification at NASA and requires a unique approach that spans the professional life-span of all active astronauts. TOPIC: This presentation will address the Behavioral Health and Performance (BHP) operations program at the Johnson Space Center. Components of BHP operations include astronaut selection, as well as annual, elective, preflight, inflight, and postflight BHP assessments. Each aspect of the BHP operations program will be discussed, with a focus on behavioral fitness determination and resultant outcomes. Specifically, astronaut selection generates a rating of suitability for long duration spaceflight as well as psychiatric qualification; annual, preflight and postflight BHP assessments provoke a decision regarding the presence of any aeromedical concerns; and inflight assessment requires a conclusion pertaining to mission impact. The combination of these elements provide for a unique, comprehensive approach to flight and spaceflight adaptability. APPLICATIONS: Attendees will understand the differing facets of NASA's comprehensive BHP operations program that occurs over the course of an astronaut's career and be able to compare and contrast this to the Adaptability Rating for Military Aviation (ARMA) and proposed models presented by others on this panel.

  18. Simulation evaluation of a low-altitude helicopter flight guidance system adapted for a helmet-mounted display

    NASA Technical Reports Server (NTRS)

    Swenson, Harry N.; Zelenka, Richard E.; Hardy, Gordon H.; Dearing, Munro G.

    1992-01-01

    A computer aiding concept for low-altitude helicopter flight was developed and evaluated in a real-time piloted simulation. The concept included an optimal control trajectory-generation algorithm based upon dynamic programming and a helmet-mounted display (HMD) presentation of a pathway-in-the-sky, a phantom aircraft, and flight-path vector/predictor guidance symbology. The trajectory-generation algorithm uses knowledge of the global mission requirements, a digital terrain map, aircraft performance capabilities, and advanced navigation information to determine a trajectory between mission way points that seeks valleys to minimize threat exposure. The pilot evaluation was conducted at NASA ARC moving base Vertical Motion Simulator (VMS) by pilots representing NASA, the U.S. Army, the Air Force, and the helicopter industry. The pilots manually tracked the trajectory generated by the algorithm utilizing the HMD symbology. The pilots were able to satisfactorily perform the tracking tasks while maintaining a high degree of awareness of the outside world.

  19. Integrated Flight/Structural Mode Control for Very Flexible Aircraft Using L1 Adaptive Output Feedback Controller

    NASA Technical Reports Server (NTRS)

    Che, Jiaxing; Cao, Chengyu; Gregory, Irene M.

    2012-01-01

    This paper explores application of adaptive control architecture to a light, high-aspect ratio, flexible aircraft configuration that exhibits strong rigid body/flexible mode coupling. Specifically, an L(sub 1) adaptive output feedback controller is developed for a semi-span wind tunnel model capable of motion. The wind tunnel mount allows the semi-span model to translate vertically and pitch at the wing root, resulting in better simulation of an aircraft s rigid body motion. The control objective is to design a pitch control with altitude hold while suppressing body freedom flutter. The controller is an output feedback nominal controller (LQG) augmented by an L(sub 1) adaptive loop. A modification to the L(sub 1) output feedback is proposed to make it more suitable for flexible structures. The new control law relaxes the required bounds on the unmatched uncertainty and allows dependence on the state as well as time, i.e. a more general unmatched nonlinearity. The paper presents controller development and simulated performance responses. Simulation is conducted by using full state flexible wing models derived from test data at 10 different dynamic pressure conditions. An L(sub 1) adaptive output feedback controller is designed for a single test point and is then applied to all the test cases. The simulation results show that the L(sub 1) augmented controller can stabilize and meet the performance requirements for all 10 test conditions ranging from 30 psf to 130 psf dynamic pressure.

  20. Flight Wing Surface Pressure and Boundary-Layer Data Report from the F-111 Smooth Variable-Camber Supercritical Mission Adaptive Wing

    NASA Technical Reports Server (NTRS)

    Powers, Sheryll Goecke; Webb, Lannie D.

    1997-01-01

    Flight tests were conducted using the advanced fighter technology integration F-111 (AFTI/F-111) aircraft modified with a variable-sweep supercritical mission adaptive wing (MAW). The MAW leading- and trailing-edge variable-camber surfaces were deflected in flight to provide a near-ideal wing camber shape for the flight condition. The MAW features smooth, flexible upper surfaces and fully enclosed lower surfaces, which distinguishes it from conventional flaps that have discontinuous surfaces and exposed or semi-exposed mechanisms. Upper and lower surface wing pressure distributions were measured along four streamwise rows on the right wing for cruise, maneuvering, and landing configurations. Boundary-layer measurements were obtained near the trailing edge for one of the rows. Cruise and maneuvering wing leading-edge sweeps were 26 deg for Mach numbers less than 1 and 45 deg or 58 deg for Mach numbers greater than 1. The landing wing sweep was 9 deg or 16 deg. Mach numbers ranged from 0.27 to 1.41, angles of attack from 2 deg to 13 deg, and Reynolds number per unit foot from 1.4 x 10(exp 6) to 6.5 x 10(exp 6). Leading-edge cambers ranged from O deg to 20 deg down, and trailing-edge cambers ranged from 1 deg up to 19 deg down. Wing deflection data for a Mach number of 0.85 are shown for three cambers. Wing pressure and boundary-layer data are given. Selected data comparisons are shown. Measured wing coordinates are given for three streamwise semispan locations for cruise camber and one spanwise location for maneuver camber.

  1. Flight control actuation system

    NASA Technical Reports Server (NTRS)

    Wingett, Paul T. (Inventor); Gaines, Louie T. (Inventor); Evans, Paul S. (Inventor); Kern, James I. (Inventor)

    2006-01-01

    A flight control actuation system comprises a controller, electromechanical actuator and a pneumatic actuator. During normal operation, only the electromechanical actuator is needed to operate a flight control surface. When the electromechanical actuator load level exceeds 40 amps positive, the controller activates the pneumatic actuator to offset electromechanical actuator loads to assist the manipulation of flight control surfaces. The assistance from the pneumatic load assist actuator enables the use of an electromechanical actuator that is smaller in size and mass, requires less power, needs less cooling processes, achieves high output forces and adapts to electrical current variations. The flight control actuation system is adapted for aircraft, spacecraft, missiles, and other flight vehicles, especially flight vehicles that are large in size and travel at high velocities.

  2. Flight control actuation system

    NASA Technical Reports Server (NTRS)

    Wingett, Paul T. (Inventor); Gaines, Louie T. (Inventor); Evans, Paul S. (Inventor); Kern, James I. (Inventor)

    2004-01-01

    A flight control actuation system comprises a controller, electromechanical actuator and a pneumatic actuator. During normal operation, only the electromechanical actuator is needed to operate a flight control surface. When the electromechanical actuator load level exceeds 40 amps positive, the controller activates the pneumatic actuator to offset electromechanical actuator loads to assist the manipulation of flight control surfaces. The assistance from the pneumatic load assist actuator enables the use of an electromechanical actuator that is smaller in size and mass, requires less power, needs less cooling processes, achieves high output forces and adapts to electrical current variations. The flight control actuation system is adapted for aircraft, spacecraft, missiles, and other flight vehicles, especially flight vehicles that are large in size and travel at high velocities.

  3. Adaptive infinite impulse response system identification using modified-interior search algorithm with Lèvy flight.

    PubMed

    Kumar, Manjeet; Rawat, Tarun Kumar; Aggarwal, Apoorva

    2017-03-01

    In this paper, a new meta-heuristic optimization technique, called interior search algorithm (ISA) with Lèvy flight is proposed and applied to determine the optimal parameters of an unknown infinite impulse response (IIR) system for the system identification problem. ISA is based on aesthetics, which is commonly used in interior design and decoration processes. In ISA, composition phase and mirror phase are applied for addressing the nonlinear and multimodal system identification problems. System identification using modified-ISA (M-ISA) based method involves faster convergence, single parameter tuning and does not require derivative information because it uses a stochastic random search using the concepts of Lèvy flight. A proper tuning of control parameter has been performed in order to achieve a balance between intensification and diversification phases. In order to evaluate the performance of the proposed method, mean square error (MSE), computation time and percentage improvement are considered as the performance measure. To validate the performance of M-ISA based method, simulations has been carried out for three benchmarked IIR systems using same order and reduced order system. Genetic algorithm (GA), particle swarm optimization (PSO), cat swarm optimization (CSO), cuckoo search algorithm (CSA), differential evolution using wavelet mutation (DEWM), firefly algorithm (FFA), craziness based particle swarm optimization (CRPSO), harmony search (HS) algorithm, opposition based harmony search (OHS) algorithm, hybrid particle swarm optimization-gravitational search algorithm (HPSO-GSA) and ISA are also used to model the same examples and simulation results are compared. Obtained results confirm the efficiency of the proposed method.

  4. Flow velocity profiling using acoustic time of flight flow metering based on wide band signals and adaptive beam-forming techniques

    NASA Astrophysics Data System (ADS)

    Murgan, I.; Candel, I.; Ioana, C.; Digulescu, A.; Bunea, F.; Ciocan, G. D.; Anghel, A.; Vasile, G.

    2016-11-01

    In this paper, we present a novel approach to non-intrusive flow velocity profiling technique using multi-element sensor array and wide-band signal's processing methods. Conventional techniques for the measurements of the flow velocity profiles are usually based on intrusive instruments (current meters, acoustic Doppler profilers, Pitot tubes, etc.) that take punctual velocity readings. Although very efficient, these choices are limited in terms of practical cases of applications especially when non-intrusive measurements techniques are required and/or a spatial accuracy of the velocity profiling is required This is due to factors related to hydraulic machinery down time, the often long time duration needed to explore the entire section area, the frequent cumbersome number of devices that needs to be handled simultaneously, or the impossibility to perform intrusive tests. In the case of non-intrusive flow profiling methods based on acoustic techniques, previous methods concentrated on using a large number of acoustic transducers placed around the measured section. Although feasible, this approach presents several major drawbacks such as a complicated signal timing, transmission, acquisition and recording system, resulting in a relative high cost of operation. In addition, because of the geometrical constraints, a desired number of sensors may not be installed. Recent results in acoustic flow metering based on wide band signals and adaptive beamforming proved that it is possible to achieve flow velocity profiles using less acoustic transducers. In a normal acoustic time of flight path the transducers are both emitters and receivers, sequentially changing their roles. In the new configuration, proposed in this paper, two new receivers are added on each side. Since the beam angles of each acoustic transducer are wide enough the newly added transducers can receive the transmitted signals and additional time of flight estimation can be done. Thus, several flow

  5. Non-destructive determination of Malondialdehyde (MDA) distribution in oilseed rape leaves by laboratory scale NIR hyperspectral imaging

    PubMed Central

    Kong, Wenwen; Liu, Fei; Zhang, Chu; Zhang, Jianfeng; Feng, Hailin

    2016-01-01

    The feasibility of hyperspectral imaging with 400–1000 nm was investigated to detect malondialdehyde (MDA) content in oilseed rape leaves under herbicide stress. After comparing the performance of different preprocessing methods, linear and nonlinear calibration models, the optimal prediction performance was achieved by extreme learning machine (ELM) model with only 23 wavelengths selected by competitive adaptive reweighted sampling (CARS), and the result was RP = 0.929 and RMSEP = 2.951. Furthermore, MDA distribution map was successfully achieved by partial least squares (PLS) model with CARS. This study indicated that hyperspectral imaging technology provided a fast and nondestructive solution for MDA content detection in plant leaves. PMID:27739491

  6. Non-destructive determination of Malondialdehyde (MDA) distribution in oilseed rape leaves by laboratory scale NIR hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Kong, Wenwen; Liu, Fei; Zhang, Chu; Zhang, Jianfeng; Feng, Hailin

    2016-10-01

    The feasibility of hyperspectral imaging with 400–1000 nm was investigated to detect malondialdehyde (MDA) content in oilseed rape leaves under herbicide stress. After comparing the performance of different preprocessing methods, linear and nonlinear calibration models, the optimal prediction performance was achieved by extreme learning machine (ELM) model with only 23 wavelengths selected by competitive adaptive reweighted sampling (CARS), and the result was RP = 0.929 and RMSEP = 2.951. Furthermore, MDA distribution map was successfully achieved by partial least squares (PLS) model with CARS. This study indicated that hyperspectral imaging technology provided a fast and nondestructive solution for MDA content detection in plant leaves.

  7. Intelligent flight control systems

    NASA Technical Reports Server (NTRS)

    Stengel, Robert F.

    1993-01-01

    The capabilities of flight control systems can be enhanced by designing them to emulate functions of natural intelligence. Intelligent control functions fall in three categories. Declarative actions involve decision-making, providing models for system monitoring, goal planning, and system/scenario identification. Procedural actions concern skilled behavior and have parallels in guidance, navigation, and adaptation. Reflexive actions are spontaneous, inner-loop responses for control and estimation. Intelligent flight control systems learn knowledge of the aircraft and its mission and adapt to changes in the flight environment. Cognitive models form an efficient basis for integrating 'outer-loop/inner-loop' control functions and for developing robust parallel-processing algorithms.

  8. Spacelab 3 flight experiment No. 3AFT23: Autogenic-feedback training as a preventive method for space adaptation syndrome

    NASA Technical Reports Server (NTRS)

    Cowings, Patricia S.; Toscano, William B.; Kamiya, Joe; Miller, Neal E.; Sharp, Joseph C.

    1988-01-01

    Space adaptation syndrome is a motion sickness-like disorder which affects up to 50 percent of all people exposed to microgravity in space. This experiment tested a physiological conditioning procedure (Autogenic-Feedback Training, AFT) as an alternative to pharmacological management. Four astronauts participated as subjects in this experiment. Crewmembers A and B served as treatment subjects. Both received preflight training for control of heart rate, respiration rate, peripheral blood volume, and skin conductance. Crewmembers C and D served as controls (i.e., did not receive training). Crewmember A showed reliable control of his own physiological responses, and a significant increase in motion sickness tolerance after training. Crewmember B, however, demonstrated much less control and only a moderate increase in motion sickness tolerance was observed after training. The inflight symptom reports and physiological data recordings revealed that Crewmember A did not experience any severe symptom episodes during the mission, while Crewmember B reported one severe symptom episode. Both control group subjects, C and D (who took antimotion sickness medication), reported multiple symptom episodes on mission day 0. Both inflight data and crew reports indicate that AFT may be an effective countermeasure. Additional data must be obtained inflight (a total of eight treatment and eight control subjects) before final evaluation of this treatment can be made.

  9. YF-17 in Flight

    NASA Technical Reports Server (NTRS)

    1976-01-01

    The Northrop Aviation YF-17 technology demonstrator aircraft in flight during a 1976 flight research program at NASA's Dryden Flight Research Center, Edwards, California. From May 27 to July 14, 1976, the Dryden Flight Research Center, Edwards, California, flew the Northrop Aviation YF-17 technology demonstrator to test the high-performance U.S. Air Force fighter at transonic speeds. The objectives of the seven-week flight test program included the study of maneuverability of this aircraft at transonic speeds and the collection of in-flight pressure data from around the afterbody of the aircraft to improve wind-tunnel predictions for future fighter aircraft. Also studied were stability and control and buffeting at high angles of attack as well as handling qualities at high load factors. Another objective of this program was to familiarize center pilots with the operation of advanced high-performance fighter aircraft. During the seven-week program, all seven of the center's test pilots were able to fly the aircraft with Gary Krier serving as project pilot. In general the pilots reported no trouble adapting to the aircraft and reported that it was easy to fly. There were no familiarization flights. All 25 research flights were full-data flights. They obtained data on afterbody pressures, vertical-fin dynamic loads, agility, pilot physiology, and infrared signatures. Average flight time was 45 minutes, although two flights involving in-flight refueling lasted approximately one hour longer than usual. Dryden Project Manager Roy Bryant considered the program a success. Center pilots felt that the aircraft was generations ahead of then current active military aircraft. Originally built for the Air Force's lightweight fighter program, the YF-17 Cobra left Dryden to support the Northrop/Navy F-18 Program. The F-18 Hornet evolved from the YF-17.

  10. Anti-MDA5 dermatomyositis mimicking psoriatic arthritis.

    PubMed

    Cabezas-Rodríguez, Iván; Morante-Bolado, Isla; Brandy-García, Anahy; Queiro-Silva, Rubén; Mozo, Lourdes; Ballina-García, Francisco Javier

    2016-12-28

    Dermatomyositis causes inflammation and damage of muscle and skin, and sometimes involves internal organs, especially lung parenchyma. Patients with dermatomyositis still represent a diagnostic challenge because of the rarity of this disease and the lack of specificity of some of its cutaneous manifestations. Herein, we describe the case of a patient with dermatomyositis, initially diagnosed as psoriatic arthritis, in which the performance of anti-melanoma differentiation-associated gene 5 (MDA5) antibodies was decisive to establish a definitive diagnosis.

  11. UV Decontamination of MDA Reagents for Single Cell Genomics

    SciTech Connect

    Lee, Janey; Tighe, Damon; Sczyrba, Alexander; Malmatrom, Rex; Clingenpeel, Scott; Malfatti, Stephanie; Rinke, Christian; Wang, Zhong; Stepanauskas, Ramunas; Cheng, Jan-Fang; Woyke, Tanja

    2011-03-18

    Single cell genomics, the amplification and sequencing of genomes from single cells, can provide a glimpse into the genetic make-up and thus life style of the vast majority of uncultured microbial cells, making it an immensely powerful and increasingly popular tool. This is accomplished by use of multiple displacement amplification (MDA), which can generate billions of copies of a single bacterial genome producing microgram-range DNA required for shotgun sequencing. Here, we address a key challenge inherent to this approach and propose a solution for the improved recovery of single cell genomes. While DNA-free reagents for the amplification of a single cell genome are a prerequisite for successful single cell sequencing and analysis, DNA contamination has been detected in various reagents, which poses a considerable challenge. Our study demonstrates the effect of UV irradiation in efficient elimination of exogenous contaminant DNA found in MDA reagents, while maintaining Phi29 activity. Consequently, we also find that increased UV exposure to Phi29 does not adversely affect genome coverage of MDA amplified single cells. While additional challenges in single cell genomics remain to be resolved, the proposed methodology is relatively quick and simple and we believe that its application will be of high value for future single cell sequencing projects.

  12. Cardiovascular adaptations in weightlessness: The influence of in-flight exercise programs on the cardiovascular adjustments during weightlessness and upon returning to Earth

    NASA Technical Reports Server (NTRS)

    Bennett, C. H.

    1981-01-01

    The effect of in-flight exercise programs on astronauts' cardiovascular adjustments during spaceflight weightlessness and upon return to Earth was studied. Physiological changes in muscle strength and volume, cardiovascular responses during the application of lower body negative pressure, and metabolic activities during pre-flight and flight tests were made on Skylab crewmembers. The successful completion of the Skylab missions showed that man can perform submaximal and maximal aerobic exercise in the weightless enviroment without detrimental trends in any of the physiologic data. Exercise tolerance during flight was unaffected. It was only after return to Earth that a tolerance decrement was noted. The rapid postflight recovery of orthostatic and exercise tolerance following two of the three Skylab missions appeared to be directly related to total in-flight exercise as well as to the graded, regular program of exercise performed during the postflight debriefing period.

  13. In-Flight System Identification

    NASA Technical Reports Server (NTRS)

    Morelli, Eugene A.

    1998-01-01

    A method is proposed and studied whereby the system identification cycle consisting of experiment design and data analysis can be repeatedly implemented aboard a test aircraft in real time. This adaptive in-flight system identification scheme has many advantages, including increased flight test efficiency, adaptability to dynamic characteristics that are imperfectly known a priori, in-flight improvement of data quality through iterative input design, and immediate feedback of the quality of flight test results. The technique uses equation error in the frequency domain with a recursive Fourier transform for the real time data analysis, and simple design methods employing square wave input forms to design the test inputs in flight. Simulation examples are used to demonstrate that the technique produces increasingly accurate model parameter estimates resulting from sequentially designed and implemented flight test maneuvers. The method has reasonable computational requirements, and could be implemented aboard an aircraft in real time.

  14. Design and flight test of the Propulsion Controlled Aircraft (PCA) flight control system on the NASA F-15 test aircraft

    NASA Technical Reports Server (NTRS)

    Wells, Edward A.; Urnes, James M., Sr.

    1994-01-01

    This report describes the design, development and flight testing of the Propulsion Controlled Aircraft (PCA) flight control system performed at McDonnell Douglas Aerospace (MDA), St. Louis, Missouri and at the NASA Dryden Flight Research Facility, Edwards Air Force Base, California. This research and development program was conducted by MDA and directed by NASA through the Dryden Flight Research Facility for the period beginning January 1991 and ending December 1993. A propulsion steering backup to the aircraft conventional flight control system has been developed and flight demonstrated on a NASA F-15 test aircraft. The Propulsion Controlled Aircraft (PCA) flight system utilizes collective and differential thrust changes to steer an aircraft that experiences partial or complete failure of the hydraulically actuated control surfaces. The PCA flight control research has shown that propulsion steering is a viable backup flight control mode and can assist the pilot in safe landing recovery of a fighter aircraft that has damage to or loss of the flight control surfaces. NASA, USAF and Navy evaluation test pilots stated that the F-15 PCA design provided the control necessary to land the aircraft. Moreover, the feasibility study showed that PCA technology can be directly applied to transport aircraft and provide a major improvement in the survivability of passengers and crew of controls damaged aircraft.

  15. Novel mechanism of MDA-7/IL-24 cancer-specific apoptosis through SARI induction

    PubMed Central

    Dash, Rupesh; Bhoopathi, Praveen; Das, Swadesh K.; Sarkar, Siddik; Emdad, Luni; Dasgupta, Santanu; Sarkar, Devanand; Fisher, Paul B.

    2014-01-01

    Subtraction-hybridization combined with induction of cancer cell terminal differentiation in human melanoma cells identified melanoma differentiation associated gene-7 (mda-7/IL-24) and SARI (Suppressor of AP-1, induced by IFN) that display potent antitumor activity. These genes are not constitutively expressed in cancer cells and forced expression of mda-7/IL-24 (Ad.mda-7) or SARI(Ad.SARI) promotes cancer-specific cell death. Ectopic expression of mda-7/IL-24 induces SARI mRNA and protein in a panel of different cancer cells leading to cell death, without harming corresponding normal cells. Simultaneous inhibition of K-ras downstream extracellular regulated kinase 1/2 (ERK1/2) signaling in pancreatic cancer cells reverses the translational block of MDA-7/IL-24 and induces SARI expression and cell death. Using SARI-antisense-based approaches we demonstrate that SARI expression is necessary for mda-7/IL-24 antitumor effects. Secreted MDA-7/IL-24 protein induces antitumor ‘bystander’ effects by promoting its own expression. Recombinant MDA-7/IL-24 (His-MDA-7) induces SARI expression, supporting the involvement of SARI in the MDA-7/IL-24-driven autocrine loop culminating in antitumor effects. Moreover, His-MDA-7 after binding to its cognate receptors (IL-20R1/IL-20R2 or IL-22R/IL-20R2) induces intracellular signaling by phosphorylation of p38 MAPK leading to transcription of a family of growth arrest and DNA damage inducible (GADD) genes, culminating in apoptosis. Inhibition of p38 MAPK fails to induce SARI following Ad.mda-7 infection. These findings reveal the significance of the mda-7/IL-24-SARI axis in cancer-specific killing, and provide a potential strategy for treating both local and metastatic disease. PMID:24282278

  16. Loquat (Eriobotrya japonica) leaf extract inhibits the growth of MDA-MB-231 tumors in nude mouse xenografts and invasion of MDA-MB-231 cells

    PubMed Central

    You, Mi-Kyoung; Kim, Min-Sook; Jeong, Kyu-Shik; Kim, Eun; Kim, Yong-Jae

    2016-01-01

    BACKGROUND/OBJECTIVES The present study was conducted to examine the inhibitory effect of loquat leaves on MDA-MB-231 cell proliferation and invasion. MATERIALS/METHODS Female athymic nude mice were given a subcutaneous (s.c.) inoculation of MDA-MB-231 cells and randomly grouped to receive a s.c. injection of either 500 mg/kg ethanol, water extract or vehicle five times a week. Tumor growth, mitotic rate and necrosis were examined. MDA-MB-231 cells were cultured with DMSO or with various concentrations of loquat water or ethanol extract. Proliferation, adhesion, migration, invasion and matrix metalloproteinase (MMP) activity were examined. RESULTS Tumor growth of xenograft nude mouse was significantly reduced by loquat extracts. The results of mitotic examination revealed that loquat extracts reduced tumor cell division. Both ethanol and water extracts significantly inhibited MDA-MB-231 cell proliferation. The protein expression of ErbB3 was significantly down-regulated by loquat leaf extracts. Loquat leaf extracts increased apoptosis of MDA-MB-231 cells following 24 hour incubation and the ethanol extract was more potent in inducing apoptosis than the water extract. Furthermore, loquat extracts inhibited adhesion, migration and invasion of MDA-MB-231 cells. MMP activity was significantly inhibited by loquat extracts. CONCLUSION Our results show that extracts of loquat inhibit the growth of tumor in MDA-MB-231 xenograft nude mice and the invasion of human breast cancer cells, indicating the inhibition of tumor cell proliferation and invasion. PMID:27087896

  17. Adaptive beam-width control of echolocation sounds by CF-FM bats, Rhinolophus ferrumequinum nippon, during prey-capture flight.

    PubMed

    Matsuta, Naohiro; Hiryu, Shizuko; Fujioka, Emyo; Yamada, Yasufumi; Riquimaroux, Hiroshi; Watanabe, Yoshiaki

    2013-04-01

    The echolocation sounds of Japanese CF-FM bats (Rhinolophus ferrumequinum nippon) were measured while the bats pursued a moth (Goniocraspidum pryeri) in a flight chamber. Using a 31-channel microphone array system, we investigated how CF-FM bats adjust pulse direction and beam width according to prey position. During the search and approach phases, the horizontal and vertical beam widths were ±22±5 and ±13±5 deg, respectively. When bats entered the terminal phase approximately 1 m from a moth, distinctive evasive flight by G. pryeri was sometimes observed. Simultaneously, the bats broadened the beam widths of some emissions in both the horizontal (44% of emitted echolocation pulses) and vertical planes (71%). The expanded beam widths were ±36±7 deg (horizontal) and ±30±9 deg (vertical). When moths began evasive flight, the tracking accuracy decreased compared with that during the approach phase. However, in 97% of emissions during the terminal phase, the beam width was wider than the misalignment (the angular difference between the pulse and target directions). These findings indicate that bats actively adjust their beam width to retain the moving target within a spatial echolocation window during the final capture stages.

  18. Integrated Resilient Aircraft Control Project Full Scale Flight Validation

    NASA Technical Reports Server (NTRS)

    Bosworth, John T.

    2009-01-01

    Objective: Provide validation of adaptive control law concepts through full scale flight evaluation. Technical Approach: a) Engage failure mode - destabilizing or frozen surface. b) Perform formation flight and air-to-air tracking tasks. Evaluate adaptive algorithm: a) Stability metrics. b) Model following metrics. Full scale flight testing provides an ability to validate different adaptive flight control approaches. Full scale flight testing adds credence to NASA's research efforts. A sustained research effort is required to remove the road blocks and provide adaptive control as a viable design solution for increased aircraft resilience.

  19. Orion Abort Flight Test

    NASA Technical Reports Server (NTRS)

    Hayes, Peggy Sue

    2010-01-01

    The purpose of NASA's Constellation project is to create the new generation of spacecraft for human flight to the International Space Station in low-earth orbit, the lunar surface, as well as for use in future deep-space exploration. One portion of the Constellation program was the development of the Orion crew exploration vehicle (CEV) to be used in spaceflight. The Orion spacecraft consists of a crew module, service module, space adapter and launch abort system. The crew module was designed to hold as many as six crew members. The Orion crew exploration vehicle is similar in design to the Apollo space capsules, although larger and more massive. The Flight Test Office is the responsible flight test organization for the launch abort system on the Orion crew exploration vehicle. The Flight Test Office originally proposed six tests that would demonstrate the use of the launch abort system. These flight tests were to be performed at the White Sands Missile Range in New Mexico and were similar in nature to the Apollo Little Joe II tests performed in the 1960s. The first flight test of the launch abort system was a pad abort (PA-1), that took place on 6 May 2010 at the White Sands Missile Range in New Mexico. Primary flight test objectives were to demonstrate the capability of the launch abort system to propel the crew module a safe distance away from a launch vehicle during a pad abort, to demonstrate the stability and control characteristics of the vehicle, and to determine the performance of the motors contained within the launch abort system. The focus of the PA-1 flight test was engineering development and data acquisition, not certification. In this presentation, a high level overview of the PA-1 vehicle is given, along with an overview of the Mobile Operations Facility and information on the White Sands tracking sites for radar & optics. Several lessons learned are presented, including detailed information on the lessons learned in the development of wind

  20. The effects of stress hormones on immune function may be vital for the adaptive reconfiguration of the immune system during fight-or-flight behavior.

    PubMed

    Adamo, Shelley A

    2014-09-01

    Intense, short-term stress (i.e., robust activation of the fight-or-flight response) typically produces a transient decline in resistance to disease in animals across phyla. Chemical mediators of the stress response (e.g., stress hormones) help induce this decline, suggesting that this transient immunosuppression is an evolved response. However, determining the function of stress hormones on immune function is difficult because of their complexity. Nevertheless, evidence suggests that stress hormones help maintain maximal resistance to disease during the physiological changes needed to optimize the body for intense physical activity. Work on insects demonstrates that stress hormones both shunt resources away from the immune system during fight-or-flight responses as well as reconfigure the immune system. Reconfiguring the immune system minimizes the impact of the loss of these resources and reduces the increased costs of some immune functions due to the physiological changes demanded by the fight-or-flight response. For example, during the stress response of the cricket Gryllus texensis, some molecular resources are shunted away from the immune system and toward lipid transport, resulting in a reduction in resistance to disease. However, insects' immune cells (hemocytes) have receptors for octopamine (the insect stress neurohormone). Octopamine increases many hemocyte functions, such as phagocytosis, and these changes would tend to mitigate the decline in immunity due to the loss of molecular resources. Moreover, because the stress response generates oxidative stress, some immune responses are probably more costly when activated during a stress response (e.g., those that produce reactive molecules). Some of these immune responses are depressed during stress in crickets, while others, whose costs are probably not increased during a stress response, are enhanced. Some effects of stress hormones on immune systems may be better understood as examples of reconfiguration

  1. Engineering study for pallet adapting the Apollo laser altimeter and photographic camera system for the Lidar Test Experiment on orbital flight tests 2 and 4

    NASA Technical Reports Server (NTRS)

    Kuebert, E. J.

    1977-01-01

    A Laser Altimeter and Mapping Camera System was included in the Apollo Lunar Orbital Experiment Missions. The backup system, never used in the Apollo Program, is available for use in the Lidar Test Experiments on the STS Orbital Flight Tests 2 and 4. Studies were performed to assess the problem associated with installation and operation of the Mapping Camera System in the STS. They were conducted on the photographic capabilities of the Mapping Camera System, its mechanical and electrical interface with the STS, documentation, operation and survivability in the expected environments, ground support equipment, test and field support.

  2. Lessons Learned and Flight Results from the F15 Intelligent Flight Control System Project

    NASA Technical Reports Server (NTRS)

    Bosworth, John

    2006-01-01

    A viewgraph presentation on the lessons learned and flight results from the F15 Intelligent Flight Control System (IFCS) project is shown. The topics include: 1) F-15 IFCS Project Goals; 2) Motivation; 3) IFCS Approach; 4) NASA F-15 #837 Aircraft Description; 5) Flight Envelope; 6) Limited Authority System; 7) NN Floating Limiter; 8) Flight Experiment; 9) Adaptation Goals; 10) Handling Qualities Performance Metric; 11) Project Phases; 12) Indirect Adaptive Control Architecture; 13) Indirect Adaptive Experience and Lessons Learned; 14) Gen II Direct Adaptive Control Architecture; 15) Current Status; 16) Effect of Canard Multiplier; 17) Simulated Canard Failure Stab Open Loop; 18) Canard Multiplier Effect Closed Loop Freq. Resp.; 19) Simulated Canard Failure Stab Open Loop with Adaptation; 20) Canard Multiplier Effect Closed Loop with Adaptation; 21) Gen 2 NN Wts from Simulation; 22) Direct Adaptive Experience and Lessons Learned; and 23) Conclusions

  3. Understanding Flight

    SciTech Connect

    Anderson, David

    2001-01-31

    Through the years the explanation of flight has become mired in misconceptions that have become dogma. Wolfgang Langewiesche, the author of 'Stick and Rudder' (1944) got it right when he wrote: 'Forget Bernoulli's Theorem'. A wing develops lift by diverting (from above) a lot of air. This is the same way that a propeller produces thrust and a helicopter produces lift. Newton's three laws and a phenomenon called the Coanda effect explain most of it. With an understanding of the real physics of flight, many things become clear. Inverted flight, symmetric wings, and the flight of insects are obvious. It is easy to understand the power curve, high-speed stalls, and the effect of load and altitude on the power requirements for lift. The contribution of wing aspect ratio on the efficiency of a wing, and the true explanation of ground effect will also be discussed.

  4. Digital Droplet Multiple Displacement Amplification (ddMDA) for Whole Genome Sequencing of Limited DNA Samples

    PubMed Central

    Rhee, Minsoung; Light, Yooli K.; Meagher, Robert J.; Singh, Anup K.

    2016-01-01

    Multiple displacement amplification (MDA) is a widely used technique for amplification of DNA from samples containing limited amounts of DNA (e.g., uncultivable microbes or clinical samples) before whole genome sequencing. Despite its advantages of high yield and fidelity, it suffers from high amplification bias and non-specific amplification when amplifying sub-nanogram of template DNA. Here, we present a microfluidic digital droplet MDA (ddMDA) technique where partitioning of the template DNA into thousands of sub-nanoliter droplets, each containing a small number of DNA fragments, greatly reduces the competition among DNA fragments for primers and polymerase thereby greatly reducing amplification bias. Consequently, the ddMDA approach enabled a more uniform coverage of amplification over the entire length of the genome, with significantly lower bias and non-specific amplification than conventional MDA. For a sample containing 0.1 pg/μL of E. coli DNA (equivalent of ~3/1000 of an E. coli genome per droplet), ddMDA achieves a 65-fold increase in coverage in de novo assembly, and more than 20-fold increase in specificity (percentage of reads mapping to E. coli) compared to the conventional tube MDA. ddMDA offers a powerful method useful for many applications including medical diagnostics, forensics, and environmental microbiology. PMID:27144304

  5. Digital droplet multiple displacement amplification (ddMDA) for whole genome sequencing of limited DNA samples

    SciTech Connect

    Rhee, Minsoung; Light, Yooli K.; Meagher, Robert J.; Singh, Anup K.; Kumar-Sinha, Chandan

    2016-05-04

    Here, multiple displacement amplification (MDA) is a widely used technique for amplification of DNA from samples containing limited amounts of DNA (e.g., uncultivable microbes or clinical samples) before whole genome sequencing. Despite its advantages of high yield and fidelity, it suffers from high amplification bias and non-specific amplification when amplifying sub-nanogram of template DNA. Here, we present a microfluidic digital droplet MDA (ddMDA) technique where partitioning of the template DNA into thousands of sub-nanoliter droplets, each containing a small number of DNA fragments, greatly reduces the competition among DNA fragments for primers and polymerase thereby greatly reducing amplification bias. Consequently, the ddMDA approach enabled a more uniform coverage of amplification over the entire length of the genome, with significantly lower bias and non-specific amplification than conventional MDA. For a sample containing 0.1 pg/μL of E. coli DNA (equivalent of ~3/1000 of an E. coli genome per droplet), ddMDA achieves a 65-fold increase in coverage in de novo assembly, and more than 20-fold increase in specificity (percentage of reads mapping to E. coli) compared to the conventional tube MDA. ddMDA offers a powerful method useful for many applications including medical diagnostics, forensics, and environmental microbiology.

  6. Digital droplet multiple displacement amplification (ddMDA) for whole genome sequencing of limited DNA samples

    DOE PAGES

    Rhee, Minsoung; Light, Yooli K.; Meagher, Robert J.; ...

    2016-05-04

    Here, multiple displacement amplification (MDA) is a widely used technique for amplification of DNA from samples containing limited amounts of DNA (e.g., uncultivable microbes or clinical samples) before whole genome sequencing. Despite its advantages of high yield and fidelity, it suffers from high amplification bias and non-specific amplification when amplifying sub-nanogram of template DNA. Here, we present a microfluidic digital droplet MDA (ddMDA) technique where partitioning of the template DNA into thousands of sub-nanoliter droplets, each containing a small number of DNA fragments, greatly reduces the competition among DNA fragments for primers and polymerase thereby greatly reducing amplification bias. Consequently,more » the ddMDA approach enabled a more uniform coverage of amplification over the entire length of the genome, with significantly lower bias and non-specific amplification than conventional MDA. For a sample containing 0.1 pg/μL of E. coli DNA (equivalent of ~3/1000 of an E. coli genome per droplet), ddMDA achieves a 65-fold increase in coverage in de novo assembly, and more than 20-fold increase in specificity (percentage of reads mapping to E. coli) compared to the conventional tube MDA. ddMDA offers a powerful method useful for many applications including medical diagnostics, forensics, and environmental microbiology.« less

  7. Cardiovascular function in space flight

    NASA Technical Reports Server (NTRS)

    Nicogossian, A. E.; Charles, J. B.; Bungo, M. W.; Leach-Huntoon, C. S.

    1990-01-01

    Postflight orthostatic intolerance and cardiac hemodynamics associated with manned space flight have been investigated on seven STS missions. Orthostatic heart rates appear to be influenced by the mission duration. The rates increase during the first 7-10 days of flight and recover partially after that. Fluid loading is used as a countermeasure to the postflight orthostatic intolerance. The carotid baroreceptor function shows only slight responsiveness to orthostatic stimulation. Plots of the baroreceptor function are presented. It is concluded that an early adaptation to the space flight conditions involves a fluid shift and that the subsequent alterations in the neutral controlling mechanisms contribute to the orthoststic intolerance.

  8. Phenotypic Switch Induced by Simulated Microgravity on MDA-MB-231 Breast Cancer Cells

    PubMed Central

    Masiello, Maria Grazia; Cucina, Alessandra; Proietti, Sara; Palombo, Alessandro; Coluccia, Pierpaolo; D'Anselmi, Fabrizio; Dinicola, Simona; Pasqualato, Alessia; Morini, Veronica; Bizzarri, Mariano

    2014-01-01

    Microgravity exerts dramatic effects on cell morphology and functions, by disrupting cytoskeleton and adhesion structures, as well as by interfering with biochemical pathways and gene expression. Impairment of cells behavior has both practical and theoretical significance, given that investigations of mechanisms involved in microgravity-mediated effects may shed light on how biophysical constraints cooperate in shaping complex living systems. By exposing breast cancer MDA-MB-231 cells to simulated microgravity (~0.001 g), we observed the emergence of two morphological phenotypes, characterized by distinct membrane fractal values, surface area, and roundness. Moreover, the two phenotypes display different aggregation profiles and adherent behavior on the substrate. These morphological differences are mirrored by the concomitant dramatic functional changes in cell processes (proliferation and apoptosis) and signaling pathways (ERK, AKT, and Survivin). Furthermore, cytoskeleton undergoes a dramatic reorganization, eventually leading to a very different configuration between the two populations. These findings could be considered adaptive and reversible features, given that, by culturing microgravity-exposed cells into a normal gravity field, cells are enabled to recover their original phenotype. Overall these data outline the fundamental role gravity plays in shaping form and function in living systems. PMID:25215287

  9. Multiple Diamond Anvil (MDA) apparatus using nano-polycrystalline diamond

    NASA Astrophysics Data System (ADS)

    Irifune, T.; Kunimoto, T.; Tange, Y.; Shinmei, T.; Isobe, F.; Kurio, A.; Funakoshi, K.

    2011-12-01

    for multianvil apparatus. Various versions of the Multiple Diamond Anvil (MDA) apparatus with NPD anvils (Fig.1), amalgamated forms of MA and DAC, are currently being tested for experiments under Mbar regimes without sacrificing the advantages of MA over DAC.

  10. Do birds sleep in flight?

    NASA Astrophysics Data System (ADS)

    Rattenborg, Niels C.

    2006-09-01

    The following review examines the evidence for sleep in flying birds. The daily need to sleep in most animals has led to the common belief that birds, such as the common swift ( Apus apus), which spend the night on the wing, sleep in flight. The electroencephalogram (EEG) recordings required to detect sleep in flight have not been performed, however, rendering the evidence for sleep in flight circumstantial. The neurophysiology of sleep and flight suggests that some types of sleep might be compatible with flight. As in mammals, birds exhibit two types of sleep, slow-wave sleep (SWS) and rapid eye-movement (REM) sleep. Whereas, SWS can occur in one or both brain hemispheres at a time, REM sleep only occurs bihemispherically. During unihemispheric SWS, the eye connected to the awake hemisphere remains open, a state that may allow birds to visually navigate during sleep in flight. Bihemispheric SWS may also be possible during flight when constant visual monitoring of the environment is unnecessary. Nevertheless, the reduction in muscle tone that usually accompanies REM sleep makes it unlikely that birds enter this state in flight. Upon landing, birds may need to recover the components of sleep that are incompatible with flight. Periods of undisturbed postflight recovery sleep may be essential for maintaining adaptive brain function during wakefulness. The recent miniaturization of EEG recording devices now makes it possible to measure brain activity in flight. Determining if and how birds sleep in flight will contribute to our understanding of a largely unexplored aspect of avian behavior and may also provide insight into the function of sleep.

  11. Active Listening in a Bat Cocktail Party: Adaptive Echolocation and Flight Behaviors of Big Brown Bats, Eptesicus fuscus, Foraging in a Cluttered Acoustic Environment.

    PubMed

    Warnecke, Michaela; Chiu, Chen; Engelberg, Jonathan; Moss, Cynthia F

    2015-09-01

    In their natural environment, big brown bats forage for small insects in open spaces, as well as in vegetation and in the presence of acoustic clutter. While searching and hunting for prey, bats experience sonar interference, not only from densely cluttered environments, but also from calls of conspecifics foraging in close proximity. Previous work has shown that when two bats compete for a single prey item in a relatively open environment, one of the bats may go silent for extended periods of time, which can serve to minimize sonar interference between conspecifics. Additionally, pairs of big brown bats have been shown to adjust frequency characteristics of their vocalizations to avoid acoustic interference in echo processing. In this study, we extended previous work by examining how the presence of conspecifics and environmental clutter influence the bat's echolocation behavior. By recording multichannel audio and video data of bats engaged in insect capture in open and cluttered spaces, we quantified the bats' vocal and flight behaviors. Big brown bats flew individually and in pairs in an open and cluttered room, and the results of this study shed light on the different strategies that this species employs to negotiate a complex and dynamic environment.

  12. Flight Simulation.

    DTIC Science & Technology

    1986-09-01

    PROCEEDINGS No.408 Flight Simulation DTIC !ELECTE NOVO505s ’ D -J DISTRIBUTION AND AVAILABILITY I I •k i nimy fle-"-- THE MISSION OF AGARI) The mission of...recherche. Ie d ~veloppement et lentrainement. Les objectifs du symposium de la commmission m~canique de vol de L’AGARD 6taient de fournir une description...tttbution Availjbiily CcodeS AvailI a.- d or Dist Spe~cial FLIGHT MECHANICS PANEL OFFICERS Chairman: Dr Ing. P.Hamcl Deputy Chairman: Dr Ing. A.Filisetti

  13. Space Flight Resource Management for ISS Operations

    NASA Technical Reports Server (NTRS)

    Schmidt, Larry; Slack, Kelley; O'Keefe, William; Huning, Therese; Sipes, Walter; Holland, Albert

    2011-01-01

    This slide presentation reviews the International Space Station (ISS) Operations space flight resource management, which was adapted to the ISS from the shuttle processes. It covers crew training and behavior elements.

  14. Neural Flight Control System

    NASA Technical Reports Server (NTRS)

    Gundy-Burlet, Karen

    2003-01-01

    The Neural Flight Control System (NFCS) was developed to address the need for control systems that can be produced and tested at lower cost, easily adapted to prototype vehicles and for flight systems that can accommodate damaged control surfaces or changes to aircraft stability and control characteristics resulting from failures or accidents. NFCS utilizes on a neural network-based flight control algorithm which automatically compensates for a broad spectrum of unanticipated damage or failures of an aircraft in flight. Pilot stick and rudder pedal inputs are fed into a reference model which produces pitch, roll and yaw rate commands. The reference model frequencies and gains can be set to provide handling quality characteristics suitable for the aircraft of interest. The rate commands are used in conjunction with estimates of the aircraft s stability and control (S&C) derivatives by a simplified Dynamic Inverse controller to produce virtual elevator, aileron and rudder commands. These virtual surface deflection commands are optimally distributed across the aircraft s available control surfaces using linear programming theory. Sensor data is compared with the reference model rate commands to produce an error signal. A Proportional/Integral (PI) error controller "winds up" on the error signal and adds an augmented command to the reference model output with the effect of zeroing the error signal. In order to provide more consistent handling qualities for the pilot, neural networks learn the behavior of the error controller and add in the augmented command before the integrator winds up. In the case of damage sufficient to affect the handling qualities of the aircraft, an Adaptive Critic is utilized to reduce the reference model frequencies and gains to stay within a flyable envelope of the aircraft.

  15. X-43A Final Flight Observations

    NASA Technical Reports Server (NTRS)

    Grindle, Laurie

    2011-01-01

    The presentation will provide an overview of the final flight of the NASA X-43A project. The project consisted of three flights, two planned for Mach 7 and one for Mach 10. The first flight, conducted on June 2, 2001, was unsuccessful and resulted in a nine-month mishap investigation. A two-year return to flight effort ensued and concluded when the second Mach 7 flight was successfully conducted on March 27, 2004. The third and final flight, which occurred on November 16, 2004, was the first Mach 10 flight demonstration of an airframe-integrated, scramjet-powered, hypersonic vehicle. As such, the final flight presented first time technical challenges in addition to final flight project closeout concerns. The goals and objectives for the third flight as well as those for the project will be presented. The configuration of the Hyper-X stack including the X-43A, Hyper-X launch vehicle, and Hyper-X research vehicle adapter wil also be presented. Mission differences, vehicle modifications and lessons learned from the first and second flights as they applied to the third flight will also be discussed. Although X-43A flight 3 was always planned to be the final flight of the X-43A project, the X-43 program had two other vehicles and corresponding flight phases in X-43C and X-43B. Those other projects never manifested under the X-43 banner and X-43A flight 3 also became the final flight of X-43 program.

  16. Optimum Strategies for Selecting Descent Flight-Path Angles

    NASA Technical Reports Server (NTRS)

    Wu, Minghong G. (Inventor); Green, Steven M. (Inventor)

    2016-01-01

    An information processing system and method for adaptively selecting an aircraft descent flight path for an aircraft, are provided. The system receives flight adaptation parameters, including aircraft flight descent time period, aircraft flight descent airspace region, and aircraft flight descent flyability constraints. The system queries a plurality of flight data sources and retrieves flight information including any of winds and temperatures aloft data, airspace/navigation constraints, airspace traffic demand, and airspace arrival delay model. The system calculates a set of candidate descent profiles, each defined by at least one of a flight path angle and a descent rate, and each including an aggregated total fuel consumption value for the aircraft following a calculated trajectory, and a flyability constraints metric for the calculated trajectory. The system selects a best candidate descent profile having the least fuel consumption value while the fly ability constraints metric remains within aircraft flight descent flyability constraints.

  17. Selected Flight Test Results for Online Learning Neural Network-Based Flight Control System

    NASA Technical Reports Server (NTRS)

    Williams-Hayes, Peggy S.

    2004-01-01

    The NASA F-15 Intelligent Flight Control System project team developed a series of flight control concepts designed to demonstrate neural network-based adaptive controller benefits, with the objective to develop and flight-test control systems using neural network technology to optimize aircraft performance under nominal conditions and stabilize the aircraft under failure conditions. This report presents flight-test results for an adaptive controller using stability and control derivative values from an online learning neural network. A dynamic cell structure neural network is used in conjunction with a real-time parameter identification algorithm to estimate aerodynamic stability and control derivative increments to baseline aerodynamic derivatives in flight. This open-loop flight test set was performed in preparation for a future phase in which the learning neural network and parameter identification algorithm output would provide the flight controller with aerodynamic stability and control derivative updates in near real time. Two flight maneuvers are analyzed - pitch frequency sweep and automated flight-test maneuver designed to optimally excite the parameter identification algorithm in all axes. Frequency responses generated from flight data are compared to those obtained from nonlinear simulation runs. Flight data examination shows that addition of flight-identified aerodynamic derivative increments into the simulation improved aircraft pitch handling qualities.

  18. [Whole-genome amplification by MDA to improve sensitivity of forensic expert examination of chromosomal DNA].

    PubMed

    Ivanov, P L; Fomichev, A A

    2008-01-01

    This pilot project has the objective to evaluate the possibility of application of the multiple displacement amplification (MDA) technique to whole-genome amplification with a view to improving sensitivity of molecular-genetic test-systems. Preparations of total cellular DNA were amplified by MDA and analysed to assess conserved specificity of chromosomal DNA and its enhanced template activity in the standard polymerase chain reaction (PCR) for typing allele variants of polymorphous DNA loci. DNA samples before and after MDA showed virtually identical genotypic combinations of alleles. Allele fragments were stably detected at a level of DNA 4-5 times lower than in the standard test. The results of the study indicate that the MDA technique provides a promising tool to improve reliability of forensic- expert examination of chromosomal DNA and imply the necessity to further develop forensic-medical aspects of this method.

  19. Use of MDA (the "love drug") and methamphetamine in Toronto by unsuspecting users of ecstasy (MDMA).

    PubMed

    Kalasinsky, Kathryn S; Hugel, John; Kish, Stephen J

    2004-09-01

    It has recently been reported that purity of illicit tablets of ecstasy (MDMA) is now high. Our objective was to confirm whether hair of drug users, who request only ecstasy from their supplier, contains MDMA in the absence of other drugs. GC-MS analysis of scalp hair segments disclosed the presence of MDMA in 19 of 21 subjects and amphetamine/methamphetamine in eight subjects. Surprisingly, seven subjects had hair levels of the MDMA metabolite, MDA, equal to or greater than those of MDMA, suggesting use of MDA in addition to that of MDMA. These amphetamine derivatives might be included by clandestine laboratories to enhance effects of the drug cocktail or because of a perception that MDA synthesis might be simpler than that of MDMA. Drug users and investigators examining possible brain neurotoxic effects of MDMA need to consider that "ecstasy" tablets can contain MDA and methamphetamine despite no demand for the drugs.

  20. Inhibition of radiation-induced glioblastoma invasion by genetic and pharmacological targeting of MDA-9/Syntenin.

    PubMed

    Kegelman, Timothy P; Wu, Bainan; Das, Swadesh K; Talukdar, Sarmistha; Beckta, Jason M; Hu, Bin; Emdad, Luni; Valerie, Kristoffer; Sarkar, Devanand; Furnari, Frank B; Cavenee, Webster K; Wei, Jun; Purves, Angela; De, Surya K; Pellecchia, Maurizio; Fisher, Paul B

    2017-01-10

    Glioblastoma multiforme (GBM) is an intractable tumor despite therapeutic advances, principally because of its invasive properties. Radiation is a staple in therapeutic regimens, although cells surviving radiation can become more aggressive and invasive. Subtraction hybridization identified melanoma differentiation-associated gene 9 [MDA-9/Syntenin; syndecan-binding protein (SDCBP)] as a differentially regulated gene associated with aggressive cancer phenotypes in melanoma. MDA-9/Syntenin, a highly conserved double-PDZ domain-containing scaffolding protein, is robustly expressed in human-derived GBM cell lines and patient samples, with expression increasing with tumor grade and correlating with shorter survival times and poorer response to radiotherapy. Knockdown of MDA-9/Syntenin sensitizes GBM cells to radiation, reducing postradiation invasion gains. Radiation induces Src and EGFRvIII signaling, which is abrogated through MDA-9/Syntenin down-regulation. A specific inhibitor of MDA-9/Syntenin activity, PDZ1i (113B7), identified through NMR-guided fragment-based drug design, inhibited MDA-9/Syntenin binding to EGFRvIII, which increased following radiation. Both genetic (shmda-9) and pharmacological (PDZ1i) targeting of MDA-9/Syntenin reduced invasion gains in GBM cells following radiation. Although not affecting normal astrocyte survival when combined with radiation, PDZ1i radiosensitized GBM cells. PDZ1i inhibited crucial GBM signaling involving FAK and mutant EGFR, EGFRvIII, and abrogated gains in secreted proteases, MMP-2 and MMP-9, following radiation. In an in vivo glioma model, PDZ1i resulted in smaller, less invasive tumors and enhanced survival. When combined with radiation, survival gains exceeded radiotherapy alone. MDA-9/Syntenin (SDCBP) provides a direct target for therapy of aggressive cancers such as GBM, and defined small-molecule inhibitors such as PDZ1i hold promise to advance targeted brain cancer therapy.

  1. Influence of strong electric field on MDA and SOD of rice under atmosphere pressure

    NASA Astrophysics Data System (ADS)

    Xiong, Jianping; Hu, Shengyong; Li, Jikai; He, Songqing; Feng, Lixin

    2013-03-01

    The content of MDA is measured by TBA method in the experiment. The results show that the MDA content of rice seedlings after being radiated in a strong electric field under atmosphere pressure decreases compared to that of those not being radiated while the SOD activity decreases. It indicates that radiated seeds' resistance against oxidative stress can be greatly enhanced. The mechanism and relation between them are analyzed in this paper.

  2. Calcium Kinetics During Space Flight

    NASA Technical Reports Server (NTRS)

    Smith, Scott M.; OBrien, K. O.; Abrams, S. A.; Wastney, M. E.

    2005-01-01

    Bone loss during space flight is one of the most critical challenges to astronaut health on space exploration missions. Defining the time course and mechanism of these changes will aid in developing means to counteract bone loss during space flight, and will have relevance for other clinical situations that impair weight-bearing activity. Bone health is a product of the balance between bone formation and bone resorption. Early space research could not clearly identify which of these was the main process altered in bone loss, but identification of the collagen crosslinks in the 1990s made possible a clear understanding that the impact of space flight was greater on bone resorption, with bone formation being unchanged or only slightly decreased. Calcium kinetics data showed that bone resorption was greater during flight than before flight (668 plus or minus 130 vs. 427 plus or minus 153 mg/d, p less than 0.001), and clearly documented that true intestinal calcium absorption was lower during flight than before flight (233 plus or minus 87 vs. 460 plus or minus 47 mg/d, p less than 0.01). Weightlessness had a detrimental effect on the balance in bone turnover: the difference between daily calcium balance during flight (-234 plus or minus 102 mg/d) and calcium balance before flight (63 plus or minus 75 mg/d) approached 300 mg/d (p less than 0.01). These data demonstrate that the bone loss that occurs during space flight is a consequence of increased bone resorption and decreased intestinal calcium absorption. Examining the changes in bone and calcium homeostasis in the initial days and weeks of space flight, as well as at later times on missions longer than 6 months, is critical to understanding the nature of bone adaptation to weightlessness. To increase knowledge of these changes, we studied bone adaptation to space flight on the 16-day Space Shuttle Columbia (STS-107) mission. When the brave and talented crew of Columbia were lost during reentry on the tragic morning

  3. Influence of environmental information in natural scenes and the effects of motion adaptation on a fly motion-sensitive neuron during simulated flight

    PubMed Central

    Ullrich, Thomas W.; Kern, Roland; Egelhaaf, Martin

    2015-01-01

    ABSTRACT Gaining information about the spatial layout of natural scenes is a challenging task that flies need to solve, especially when moving at high velocities. A group of motion sensitive cells in the lobula plate of flies is supposed to represent information about self-motion as well as the environment. Relevant environmental features might be the nearness of structures, influencing retinal velocity during translational self-motion, and the brightness contrast. We recorded the responses of the H1 cell, an individually identifiable lobula plate tangential cell, during stimulation with image sequences, simulating translational motion through natural sceneries with a variety of differing depth structures. A correlation was found between the average nearness of environmental structures within large parts of the cell's receptive field and its response across a variety of scenes, but no correlation was found between the brightness contrast of the stimuli and the cell response. As a consequence of motion adaptation resulting from repeated translation through the environment, the time-dependent response modulations induced by the spatial structure of the environment were increased relatively to the background activity of the cell. These results support the hypothesis that some lobula plate tangential cells do not only serve as sensors of self-motion, but also as a part of a neural system that processes information about the spatial layout of natural scenes. PMID:25505148

  4. Identification and expression profiling analysis of goose melanoma differentiation associated gene 5 (MDA5) gene.

    PubMed

    Wei, L M; Jiao, P R; Song, Y F; Han, F; Cao, L; Yang, F; Ren, T; Liao, M

    2013-10-01

    Melanoma differentiation associated gene 5 (MDA5) is an important cytoplasmic receptor that recognizes long molecules of viral double-stranded RNA and single-stranded RNA with 5' triphosphate and mediates type I interferon secretion. In this study, the full-length MDA5 gene in the goose was identified and characterized. The cDNA of goose MDA5 was 3,306 bp in length with an open reading frame of 3,018 bp, which encoded a polypeptide of 1,005 amino acids. The deduced amino acid sequence contained 6 main structure domains including 2 caspase activation and recruitment domains, one DExD/H-box helicase domain, one type III restriction enzyme domain, one helicase conserved C-terminal domain, and one RIG-I C-terminal domain. Quantitative real-time PCR analysis indicated that goose MDA5 mRNA was constitutively expressed in all sampled tissues. It was highly expressed in the jejunum, trachea, ileum, colon, and kidney, and lowly expressed in the muscular stomach, glandular stomach, and muscle. A significant increase in the transcription of MDA5 was detected in the brain, spleen, and lungs of geese after infection with H5N1 highly pathogenic avian influenza virus compared with uninfected tissues. These findings indicated that goose MDA5 was an important receptor, involved in the antiviral innate immune defense to H5N1 highly pathogenic avian influenza virus in geese.

  5. Space flight rehabilitation.

    PubMed

    Payne, Michael W C; Williams, David R; Trudel, Guy

    2007-07-01

    The weightless environment of space imposes specific physiologic adaptations on healthy astronauts. On return to Earth, these adaptations manifest as physical impairments that necessitate a period of rehabilitation. Physiologic changes result from unloading in microgravity and highly correlate with those seen in relatively immobile terrestrial patient populations such as spinal cord, geriatric, or deconditioned bed-rest patients. Major postflight impairments requiring rehabilitation intervention include orthostatic intolerance, bone demineralization, muscular atrophy, and neurovestibular symptoms. Space agencies are preparing for extended-duration missions, including colonization of the moon and interplanetary exploration of Mars. These longer-duration flights will result in more severe and more prolonged disability, potentially beyond the point of safe return to Earth. This paper will review and discuss existing space rehabilitation plans for major postflight impairments. Evidence-based rehabilitation interventions are imperative not only to facilitate return to Earth but also to extend the safe duration of exposure to a physiologically hostile microgravity environment.

  6. Robust and reconfigurable flight control system design

    NASA Astrophysics Data System (ADS)

    Siwakosit, Wichai

    2001-07-01

    A reconfigurable flight control system is a control system which can automatically adapt itself to maintain the performance of a damaged aircraft to be as close as possible to that of the normal or undamaged one. This research focuses mainly on Multi-Input, Multi-Output (MIMO) reconfigurable flight control for an aircraft with damaged actuator(s) which may greatly affect the performance and control of the aircraft, and also pose a challenging flight control problem. The foundation of the control system is a baseline controller and an adaptive module which constitutes a reconfigurable part. The baseline controller ensures that the aircraft has acceptable performance and handling qualities throughout the flight envelope. The combination of a Quantitative Feedback Theory (QFT) Pre-Design Technique (PDT) and a Reduced-order, Linear, Dynamic Inversion (RLDI) control strategy yields a flight control system with good tracking performance and handling qualities with no Pilot Induced Oscillation (PIO) tendencies throughout the designated set of flight conditions. In addition, the system is highly immune to large uncertainties in the aircraft dynamics. The modified filtered-ɛ adaptive algorithm is developed and utilized in the adaptive module of the system. This adaptive algorithm performs well with MIMO system with the added advantage of not having to pre-identify the dynamics of the damaged aircraft, provided that the conditions of reconfigurability are met. An example of the proposed control system with the NASA F-18 HARV vehicle model and a damaged actuator demonstrates the effectiveness of the concept.

  7. In Vivo Ligands of MDA5 and RIG-I in Measles Virus-Infected Cells

    PubMed Central

    Hembach, Katharina; Baum, Alina; García-Sastre, Adolfo; Söding, Johannes; Conzelmann, Karl-Klaus

    2014-01-01

    RIG-I-like receptors (RLRs: RIG-I, MDA5 and LGP2) play a major role in the innate immune response against viral infections and detect patterns on viral RNA molecules that are typically absent from host RNA. Upon RNA binding, RLRs trigger a complex downstream signaling cascade resulting in the expression of type I interferons and proinflammatory cytokines. In the past decade extensive efforts were made to elucidate the nature of putative RLR ligands. In vitro and transfection studies identified 5′-triphosphate containing blunt-ended double-strand RNAs as potent RIG-I inducers and these findings were confirmed by next-generation sequencing of RIG-I associated RNAs from virus-infected cells. The nature of RNA ligands of MDA5 is less clear. Several studies suggest that double-stranded RNAs are the preferred agonists for the protein. However, the exact nature of physiological MDA5 ligands from virus-infected cells needs to be elucidated. In this work, we combine a crosslinking technique with next-generation sequencing in order to shed light on MDA5-associated RNAs from human cells infected with measles virus. Our findings suggest that RIG-I and MDA5 associate with AU-rich RNA species originating from the mRNA of the measles virus L gene. Corresponding sequences are poorer activators of ATP-hydrolysis by MDA5 in vitro, suggesting that they result in more stable MDA5 filaments. These data provide a possible model of how AU-rich sequences could activate type I interferon signaling. PMID:24743923

  8. NHE1 mediates MDA-MB-231 cells invasion through the regulation of MT1-MMP.

    PubMed

    Lin, Yani; Chang, Guoqiang; Wang, Jian; Jin, Weina; Wang, Lihong; Li, Huawen; Ma, Li; Li, Qinghua; Pang, Tianxiang

    2011-08-15

    Na⁺/H⁺ exchanger 1 (NHE1), an important regulator of intracellular pH (pH(i)) and extracellular pH (pH(e)), has been shown to play a key role in breast cancer metastasis. However, the exact mechanism by which NHE1 mediates breast cancer metastasis is not yet well known. We showed here that inhibition of NHE1 activity, with specific inhibitor Cariporide, could suppress MDA-MB-231 cells invasion as well as the activity and expression of MT1-MMP. Overexpression of MT1-MMP resulted in a distinguished increase in MDA-MB-231 cells invasiveness, but treatment with Cariporide reversed the MT1-MMP-mediated enhanced invasiveness. To explore the role of MAPK signaling pathways in NHE1-mediated breast cancer metastasis, we compared the difference of constitutively phosphorylated ERK1/2, p38 MAPK and JNK in non-invasive MCF-7 cells and invasive MDA-MB-231 cells. Interestingly, we found that the phosphorylation levels of ERK1/2 and p38 MAPK in MDA-MB-231 cells were higher than in MCF-7 cells, but both MCF-7 cells and MDA-MB-231 cells expressed similar constitutively phosphorylated JNK. Treating MDA-MB-231 cells with Cariporide led to decreased phosphorylation level of both p38 MAPK and ERK1/2 in a time-dependent manner, but JNK activity was not influenced. Supplementation with MAPK inhibitor (MEK inhibitor PD98059, p38 MAPK inhibitor SB203580 and JNK inhibitor SP600125) or Cariporide all exhibited significant depression of MDA-MB-231 cells invasion and MT1-MMP expression. Furthermore, we co-treated MDA-MB-231 cells with MAPK inhibitor and Cariporide. The result showed that Cariporide synergistically suppressed invasion and MT1-MMP expression with MEK inhibitor and p38 MAPK inhibitor, but not be synergistic with the JNK inhibitor. These findings suggest that NHE1 mediates MDA-MB-231 cells invasion partly through regulating MT1-MMP in ERK1/2 and p38 MAPK signaling pathways dependent manner.

  9. Both RIG-I and MDA5 detect alphavirus replication in concentration-dependent mode

    SciTech Connect

    Akhrymuk, Ivan; Frolov, Ilya; Frolova, Elena I.

    2016-01-15

    Alphaviruses are a family of positive-strand RNA viruses that circulate on all continents between mosquito vectors and vertebrate hosts. Despite a significant public health threat, their biology is not sufficiently investigated, and the mechanisms of alphavirus replication and virus–host interaction are insufficiently understood. In this study, we have applied a variety of experimental systems to further understand the mechanism by which infected cells detect replicating alphaviruses. Our new data strongly suggest that activation of the antiviral response by alphavirus-infected cells is determined by the integrity of viral genes encoding proteins with nuclear functions, and by the presence of two cellular pattern recognition receptors (PRRs), RIG-I and MDA5. No type I IFN response is induced in their absence. The presence of either of these PRRs is sufficient for detecting virus replication. However, type I IFN activation in response to pathogenic alphaviruses depends on the basal levels of RIG-I or MDA5. - Highlights: • Both RIG-I and MDA5 detect alphavirus replication. • Alphavirus-induced transcriptional shutoff affects type I IFN induction. • Sensing of alphavirus replication by RIG-I and MDA5 depends on their concentrations. • High basal level of RIG-I and MDA5 allows IFN induction by pathogenic alphaviruses. • This dependence determines the discrepancy between the in vivo and in vitro data.

  10. Utilizing wide area maritime domain awareness (MDA) data to cue a remote surveillance system

    NASA Astrophysics Data System (ADS)

    Isenor, Anthony W.; Cross, Richard; Webb, Sean; Lapinski, Anna-Liesa S.

    2013-10-01

    Defence Research and Development Canada - Atlantic (DRDC Atlantic) is currently involved in research on the topic of northern Maritime Domain Awareness (MDA). One project, entitled Situational Information for Enabling Development of Northern Awareness (SEDNA), includes research on the exploitation of MDA data in northern areas. One aspect of this research is to utilize wide area MDA data to provide awareness to an unattended, land-based system. Wide area MDA is attained through the use of space-based AIS (SAIS) data, a data feed used by the Canadian Department of National Defence and supplied by the commercial provider exactEarth Ltd. The land-based surveillance system used is the remote northern system constructed within the DRDC Northern Watch Technology Demonstration Project. Northern Watch is a multi-year project intended to show state-of-the-art, unattended, surveillance capabilities in the Canadian north. The link between the SAIS and Northern Watch is provided by a research infrastructure that consists of an assembly of data sources, users, applications, and product management techniques that collectively support research in areas such as information management and MDA data exploitation. High-level descriptions of the systems are provided along with elaboration on the alerting algorithm, the notifications that would be sent to the Northern Watch southern command site, and the resulting actions that could be taken by the Northern Watch surveillance system.

  11. Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses.

    PubMed

    Robles, Pedro; Micol, José Luis; Quesada, Víctor

    2012-01-01

    Most chloroplast and mitochondrial proteins are encoded by nuclear genes, whose functions remain largely unknown because mutant alleles are lacking. A reverse genetics screen for mutations affecting the mitochondrial transcription termination factor (mTERF) family in Arabidopsis thaliana allowed us to identify 75 lines carrying T-DNA insertions. Two of them were homozygous for insertions in the At4g14605 gene, which we dubbed MDA1 (MTERF DEFECTIVE IN Arabidopsis1). The mda1 mutants exhibited altered chloroplast morphology and plant growth, and reduced pigmentation of cotyledons, leaves, stems and sepals. The mda1 mutations enhanced salt and osmotic stress tolerance and altered sugar responses during seedling establishment, possibly as a result of reduced ABA sensitivity. Loss of MDA1 function caused up-regulation of the RpoTp/SCA3 nuclear gene encoding a plastid RNA polymerase and modified the steady-state levels of chloroplast gene transcripts. Double mutant analyses indicated that MDA1 and the previously described mTERF genes SOLDAT10 and RUG2 act in different pathways. Our findings reveal a new role for mTERF proteins in the response to abiotic stress, probably through perturbed ABA retrograde signalling resulting from a disruption in chloroplast homeostasis.

  12. Selective regain of egfr gene copies in CD44+/CD24-/low breast cancer cellular model MDA-MB-468

    PubMed Central

    2010-01-01

    Progressive genome modulation in the CD44+/CD24-/low subpopulation of the breast cancer cell line MDA-MB-468 leads to different coexisting subclones. In isolated low-copy cells asymmetric chromosomal segregation leads to new cells with regained solely egfr gene copies. Furthermore, egfr regain resulted in enhanced signal transduction of the MAP-kinase and PI3-kinase pathway. We show here for the first time a dynamic copy number regain in basal-like/stemness cell type breast cancer subpopulations which might explain genetic heterogeneity. Moreover, this process might also be involved in adaptive growth factor receptor intracellular signaling which support survival and migration during cancer development and progression. PMID:20199686

  13. Melanoma differentiation associated gene-7 (mda-7): a novel anti-tumor gene for cancer gene therapy.

    PubMed Central

    Mhashilkar, A. M.; Schrock, R. D.; Hindi, M.; Liao, J.; Sieger, K.; Kourouma, F.; Zou-Yang, X. H.; Onishi, E.; Takh, O.; Vedvick, T. S.; Fanger, G.; Stewart, L.; Watson, G. J.; Snary, D.; Fisher, P. B.; Saeki, T.; Roth, J. A.; Ramesh, R.; Chada, S.

    2001-01-01

    BACKGROUND: The mda-7 gene (melanoma differentiation associated gene-7) is a novel tumor suppressor gene. The anti-proliferative activity of MDA-7 has been previously reported. In this report, we analyze the anti-tumor efficacy of Ad-mda7 in a broad spectrum of cancer lines. MATERIALS AND METHODS: Ad-mda7-transduced cancer or normal cell lines were assayed for cell proliferation (tritiated thymidine incorporation assay, Alamar blue assay, and trypan-blue exclusion assay), apoptosis (TUNEL, and Annexin V staining visualized by fluorescent microscopy or FACs analysis), and cell cycle regulation (Propidium Iodide staining and FACs analysis). RESULTS: Ad-mda7 treatment of tumor cells resulted in growth inhibition and apoptosis in a temporal and dose-dependent manner. The anti-tumor effects were independent of the genomic status of p53, RB, p16, ras, bax, and caspase 3 in these cells. In addition, normal cell lines did not show inhibition of proliferation or apoptotic response to Ad-mda7. Moreover, Ad-mda7-transduced cancer cells secreted a soluble form of MDA-7 protein. Thus, Ad-mda7 may represent a novel gene-therapeutic agent for the treatment of a variety of cancers. CONCLUSIONS: The potent and selective killing activity of Ad-mda7 in cancer cells but not in normal cells makes this vector a potential candidate for cancer gene therapy. PMID:11471572

  14. Surface water and erosion calculations to support the MDA G performance assessment

    SciTech Connect

    Springer, E.P.

    1997-03-01

    The performance of MDA G is dependent on surface hydrological and ecological processes because radionuclide transport by surface runoff can affect human and/or environmental receptors directly and the percolation for the subsurface radionuclide transport pathway is determined by the water balance in the near surface. For subsurface disposal of waste, surface soil erosion reduces the effectiveness of the surface cover and if wastes are exposed, then surface runoff can transport contaminants either in a soluble phase or sorbed to eroded soil particles. The objectives of this section are to estimate the effects at MDA G of surface runoff, soil erosion, and percolation. The conceptual and mathematical models will be reviewed, parameter estimation for the models will be presented and results and sensitivity analyses for a surface cover at MDA G will be presented.

  15. [Effect of dietary VE on the contents of salivary acid and MDA in RBC membrane].

    PubMed

    Wang, F; Dong, Z; Zhang, Y; Chen, Y

    1997-05-01

    Vitamin E can protect membrane from the damage of lipid peroxidation, Salivary acid is the residual of carbohydrate on the membrane. To evaluate the effect of dietary VE on salivary acid, the contents of MDA and salivary acid of erythrocyte (RBC) membrane of rats were measured. The rats were fed with different amounts of dietary VE and stayed at different temperatures. The results revealed that the content of salivary acid of RBC membrane reduced markly (P < 0.01) and the content of MDA of RBC membrane was stable (P > 0.05) after the rats were exposed to cold for 10 days. High dietary VE intake increased the content of salivary acid of RBC membrane (P < 0.01). There was no correlation between the content of salivary acid and MDA of RBC membrane. It suggested that dietary VE could raise the content of salivary acid in RBC membrane, but it can not be explained by the reduction of LPO.

  16. Selected Flight Test Results for Online Learning Neural Network-Based Flight Control System

    NASA Technical Reports Server (NTRS)

    Williams, Peggy S.

    2004-01-01

    The NASA F-15 Intelligent Flight Control System project team has developed a series of flight control concepts designed to demonstrate the benefits of a neural network-based adaptive controller. The objective of the team is to develop and flight-test control systems that use neural network technology to optimize the performance of the aircraft under nominal conditions as well as stabilize the aircraft under failure conditions. Failure conditions include locked or failed control surfaces as well as unforeseen damage that might occur to the aircraft in flight. This report presents flight-test results for an adaptive controller using stability and control derivative values from an online learning neural network. A dynamic cell structure neural network is used in conjunction with a real-time parameter identification algorithm to estimate aerodynamic stability and control derivative increments to the baseline aerodynamic derivatives in flight. This set of open-loop flight tests was performed in preparation for a future phase of flights in which the learning neural network and parameter identification algorithm output would provide the flight controller with aerodynamic stability and control derivative updates in near real time. Two flight maneuvers are analyzed a pitch frequency sweep and an automated flight-test maneuver designed to optimally excite the parameter identification algorithm in all axes. Frequency responses generated from flight data are compared to those obtained from nonlinear simulation runs. An examination of flight data shows that addition of the flight-identified aerodynamic derivative increments into the simulation improved the pitch handling qualities of the aircraft.

  17. Coordinated adaptive filters for motion simulators.

    NASA Technical Reports Server (NTRS)

    Parrish, R. V.; Dieudonne, J. E.; Bowles, R. L.; Martin, D. J.

    1973-01-01

    A new approach to providing motion drive signals to a flight simulator utilizing coordinated adaptive filters is presented. Some motivation for the use of coordinated washout is discussed, along with conditions that determine the burden of coordination. The coordinated adaptive filters are derived, based on continuous steepest descent, and the application of the filters to simulated flight data is demonstrated.

  18. Green Flight Challenge

    NASA Video Gallery

    The CAFE Green Flight Challenge sponsored by Google will be held at the CAFE Foundation Flight Test Center at Charles M. Schulz Sonoma County Airport in Santa Rosa, Calif. The Green Flight Challeng...

  19. Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells.

    PubMed

    Wang, Yi; Nie, Fangyuan; Ouyang, Jian; Wang, Xiaoyan; Ma, Xiaofeng

    2014-10-01

    Fatty acid synthase (FAS) is overexpressed in many human cancers including breast cancer and is considered to be a promising target for therapy. Sea buckthorn has long been used to treat a variety of maladies. Here, we investigated the inhibitory effect of sea buckthorn procyanidins (SBPs) isolated from the seeds of sea buckthorn on FAS and FAS overexpressed human breast cancer MDA-MB-231 cells. The FAS activity and FAS inhibition were measured by a spectrophotometer at 340 nm of nicotinamide adenine dinucleotide phosphate (NADPH) absorption. We found that SBP potently inhibited the activity of FAS with a half-inhibitory concentration (IC50) value of 0.087 μg/ml. 3-4,5-Dimethylthiazol-2-yl-2,3-diphenyl tetrazolium bromide (MTT) assay was used to test the cell viability. SBP reduced MDA-MB-231 cell viability with an IC50 value of 37.5 μg/ml. Hoechst 33258/propidium iodide dual staining and flow cytometric analysis showed that SBP induced MDA-MB-231 cell apoptosis. SBP inhibited intracellular FAS activity with a dose-dependent manner. In addition, sodium palmitate could rescue the cell apoptosis induced by SBP. These results showed that SBP was a promising FAS inhibitor which could induce the apoptosis of MDA-MB-231 cells via inhibiting FAS. These findings suggested that SBP might be useful for preventing or treating breast cancer.

  20. The effect of ILLLI on peripheral blood SOD, MDA in psoriasis treatment

    NASA Astrophysics Data System (ADS)

    Zhu, Jing; Nie, Fan

    2005-07-01

    Objective: To research the effect of Intravascular low level laser irradiation (ILLLI) on the SOD,MDA in the treatment of psoriasis. Method :47 patients suffering from psoriasis from five groups were treated by Intravascular low level laser irradiation (power:4-5mw,1h per day, period of treatment: 10 days) .We checked the change of SOD,MDA peripheral blood in 10 normal people between pre and post treatment. Group A were treated by He-Ne laser combined with drug, group B were treated by semi-conductor laser combined with drug, group C were treated only by He-Ne laser, group D were treated only by semiconductor laser, group E were treated only by drug . Results: The levels of SOD in red cell of psoriatic patients from five groups after treatment were significantly lower than that of controlled group. The levels of SOD of them were significantly increased and nearly closed to that of controlled group; the levels of MDA in red cell of psoriatic patients from five groups after treatment were significantly higher than that of controlled group; the levels of MDA of them are decreased ,however, they were still not recovered to normal levels. Conclusions: ILLLI, both He-Ne laser and semiconductor laser, can activate SOD in psoriasis patients and enhance their ability of anti-oxidation.

  1. Collection and measurement of atmospheric contaminants during Skylab AM/MDA unmanned altitude chamber test

    NASA Technical Reports Server (NTRS)

    1972-01-01

    The analytical data obtained from both cryogenic and grab sampling of the atmosphere of the Skylab AM/MDA during an 84 hour unmanned chamber run are reported. The level of contaminants found at different points of the test chamber are tabulated. The results indicate that there was no clear trend of increasing or decreasing contaminant levels during the test run.

  2. Cibola flight experiment

    SciTech Connect

    Roussel-Dupre, D.; Caffrey, M. P.

    2004-01-01

    Los Alamos National Laboratory is building the Cibola Flight Experiment (CFE), a reconfigurable processor payload intended for a Low Earth Orbit system. It will survey portions of the VHF and UHF radio spectra. The experiment uses networks of reprogrammable, Field Programmable Gate Arrays (FPGAs) to process the received signals for ionospheric and lightning studies. The objective is to validate the on-orbit use of commercial, reconfigurable FPGA technology utilizing several different single-event upset mitigation schemes. It will also detect and measure impulsive events that occur in a complex background. Surrey Satellite Technology, Ltd (SSTL) is building the small host satellite, CFESat, based upon SSTL's disaster monitoring constellation (DMC) and Topsat mission satellite designs. The CFESat satellite will be launched by the Space Test Program in September 2006 on the US Air Force Evolved Expendable Launch Vehicle (EELV) using the EELV's Secondary Payload Adapter (ESPA) that allows up to six small satellites to be launched as 'piggyback' passengers with larger spacecraft.

  3. NASA's Flight Opportunities Program

    NASA Video Gallery

    NASA's Flight Opportunities Program is facilitating low-cost access to suborbital space, where researchers can test technologies using commercially developed vehicles. Suborbital flights can quickl...

  4. Development and Flight Testing of a Neural Network Based Flight Control System on the NF-15B Aircraft

    NASA Technical Reports Server (NTRS)

    Bomben, Craig R.; Smolka, James W.; Bosworth, John T.; Silliams-Hayes, Peggy S.; Burken, John J.; Larson, Richard R.; Buschbacher, Mark J.; Maliska, Heather A.

    2006-01-01

    The Intelligent Flight Control System (IFCS) project at the NASA Dryden Flight Research Center, Edwards AFB, CA, has been investigating the use of neural network based adaptive control on a unique NF-15B test aircraft. The IFCS neural network is a software processor that stores measured aircraft response information to dynamically alter flight control gains. In 2006, the neural network was engaged and allowed to learn in real time to dynamically alter the aircraft handling qualities characteristics in the presence of actual aerodynamic failure conditions injected into the aircraft through the flight control system. The use of neural network and similar adaptive technologies in the design of highly fault and damage tolerant flight control systems shows promise in making future aircraft far more survivable than current technology allows. This paper will present the results of the IFCS flight test program conducted at the NASA Dryden Flight Research Center in 2006, with emphasis on challenges encountered and lessons learned.

  5. Flight Test Series 3: Flight Test Report

    NASA Technical Reports Server (NTRS)

    Marston, Mike; Sternberg, Daniel; Valkov, Steffi

    2015-01-01

    This document is a flight test report from the Operational perspective for Flight Test Series 3, a subpart of the Unmanned Aircraft System (UAS) Integration in the National Airspace System (NAS) project. Flight Test Series 3 testing began on June 15, 2015, and concluded on August 12, 2015. Participants included NASA Ames Research Center, NASA Armstrong Flight Research Center, NASA Glenn Research Center, NASA Langley Research center, General Atomics Aeronautical Systems, Inc., and Honeywell. Key stakeholders analyzed their System Under Test (SUT) in two distinct configurations. Configuration 1, known as Pairwise Encounters, was subdivided into two parts: 1a, involving a low-speed UAS ownship and intruder(s), and 1b, involving a high-speed surrogate ownship and intruder. Configuration 2, known as Full Mission, involved a surrogate ownship, live intruder(s), and integrated virtual traffic. Table 1 is a summary of flights for each configuration, with data collection flights highlighted in green. Section 2 and 3 of this report give an in-depth description of the flight test period, aircraft involved, flight crew, and mission team. Overall, Flight Test 3 gathered excellent data for each SUT. We attribute this successful outcome in large part from the experience that was acquired from the ACAS Xu SS flight test flown in December 2014. Configuration 1 was a tremendous success, thanks to the training, member participation, integration/testing, and in-depth analysis of the flight points. Although Configuration 2 flights were cancelled after 3 data collection flights due to various problems, the lessons learned from this will help the UAS in the NAS project move forward successfully in future flight phases.

  6. Fight or flight? - Flight increases immune gene expression but does not help to fight an infection.

    PubMed

    Woestmann, L; Kvist, J; Saastamoinen, M

    2017-03-01

    Flight represents a key trait in most insects, being energetically extremely demanding, yet often necessary for foraging and reproduction. Additionally, dispersal via flight is especially important for species living in fragmented landscapes. Even though, based on life-history theory, a negative relationship may be expected between flight and immunity, a number of previous studies have indicated flight to induce an increased immune response. In this study, we assessed whether induced immunity (i.e. immune gene expression) in response to 15-min forced flight treatment impacts individual survival of bacterial infection in the Glanville fritillary butterfly (Melitaea cinxia). We were able to confirm previous findings of flight-induced immune gene expression, but still observed substantially stronger effects on both gene expression levels and life span due to bacterial infection compared to flight treatment. Even though gene expression levels of some immunity-related genes were elevated due to flight, these individuals did not show increased survival of bacterial infection, indicating that flight-induced immune activation does not completely protect them from the negative effects of bacterial infection. Finally, an interaction between flight and immune treatment indicated a potential trade-off: flight treatment increased immune gene expression in naïve individuals only, whereas in infected individuals no increase in immune gene expression was induced by flight. Our results suggest that the up-regulation of immune genes upon flight is based on a general stress response rather than reflecting an adaptive response to cope with potential infections during flight or in new habitats.

  7. Visual-Vestibular Responses During Space Flight

    NASA Technical Reports Server (NTRS)

    Reschke, M. F.; Kozlovskaya, I. B.; Paloski, W. H.

    1999-01-01

    Given the documented disruptions that occur in spatial orientation during space flight and the putative sensory-motor information underlying eye and head spatial coding, the primary purpose of this paper is to examine components of the target acquisition system in subjects free to make head and eye movements in three dimensional space both during and following adaptation to long duration space flight. It is also our intention to suggest a simple model of adaptation that has components in common with cerebellar disorders whose neurobiological substrate has been identified.

  8. Anti and Androgenic Activities in MDA-KB2 Cells: A Comparison of Performance in 96 Well Versus HTS Assays

    EPA Science Inventory

    We developed the MDA-kb2 cell line to screen androgen agonists/antagonists (Wilson et al., ToxSci 66:69, 2002). MDA-kb2 has been used to quantify anti- and androgenic activities of chemicals, mixtures, combustion by-products, oil dispersants and waste, source and drinking water s...

  9. Flight projects overview

    NASA Technical Reports Server (NTRS)

    Levine, Jack

    1988-01-01

    Information is given in viewgraph form on the activities of the Flight Projects Division of NASA's Office of Aeronautics and Space Technology. Information is given on space research and technology strategy, current space flight experiments, the Long Duration Exposure Facility, the Orbiter Experiment Program, the Lidar In-Space Technology Experiment, the Ion Auxiliary Propulsion System, the Arcjet Flight Experiment, the Telerobotic Intelligent Interface Flight Experiment, the Cryogenic Fluid Management Flight Experiment, the Industry/University In-Space Flight Experiments, and the Aeroassist Flight Experiment.

  10. Design of a Computerised Flight Mill Device to Measure the Flight Potential of Different Insects.

    PubMed

    Martí-Campoy, Antonio; Ávalos, Juan Antonio; Soto, Antonia; Rodríguez-Ballester, Francisco; Martínez-Blay, Victoria; Malumbres, Manuel Pérez

    2016-04-07

    Several insect species pose a serious threat to different plant species, sometimes becoming a pest that produces significant damage to the landscape, biodiversity, and/or the economy. This is the case of Rhynchophorus ferrugineus Olivier (Coleoptera: Dryophthoridae), Semanotus laurasii Lucas (Coleoptera: Cerambycidae), and Monochamus galloprovincialis Olivier (Coleoptera: Cerambycidae), which have become serious threats to ornamental and productive trees all over the world such as palm trees, cypresses, and pines. Knowledge about their flight potential is very important for designing and applying measures targeted to reduce the negative effects from these pests. Studying the flight capability and behaviour of some insects is difficult due to their small size and the large area wherein they can fly, so we wondered how we could obtain information about their flight capabilities in a controlled environment. The answer came with the design of flight mills. Relevant data about the flight potential of these insects may be recorded and analysed by means of a flight mill. Once an insect is attached to the flight mill, it is able to fly in a circular direction without hitting walls or objects. By adding sensors to the flight mill, it is possible to record the number of revolutions and flight time. This paper presents a full description of a computer monitored flight mill. The description covers both the mechanical and the electronic parts in detail. The mill was designed to easily adapt to the anatomy of different insects and was successfully tested with individuals from three species R. ferrugineus, S. laurasii, and M. galloprovincialis.

  11. Caffeine promotes hyperthermia and serotonergic loss following co-administration of the substituted amphetamines, MDMA ("Ecstasy") and MDA ("Love").

    PubMed

    McNamara, Ruth; Kerans, Aoife; O'Neill, Barry; Harkin, Andrew

    2006-01-01

    The present study determined the effect of caffeine co-administration on the core body temperature response and long-term serotonin (5-HT) loss induced by methylenedioxymethamphetamine (MDMA; "Ecstasy") and its metabolite methylenedioxyamphetamine (MDA; "Love") to rats. In group-housed animals, caffeine (10 mg/kg) enhanced the acute toxicity of MDMA (15 mg/kg) and MDA (7.5 mg/kg), resulting in an exaggerated hyperthermic response (+2 degrees C for 5 h following MDMA and +1.5 degrees C for 3 h following MDA) when compared to MDMA (+1 degree C for 3 h) and MDA (+1 degree C for 1 h) alone. Co-administration of caffeine with MDMA or MDA was also associated with increased lethality. To reduce the risk of lethality, doses of MDMA and MDA were reduced in further experiments and the animals were housed individually. To examine the effects of repeated administration, animals received MDMA (10 mg/kg) or MDA (5 mg/kg) with or without caffeine (10 mg/kg) twice daily for 4 consecutive days. MDMA and MDA alone induced hypothermia (fall of 1 to 2 degrees C) over the 4 treatment days. Co-administration of caffeine with MDMA or MDA resulted in hyperthermia (increase of up to 2.5 degrees C) following acute administration compared to animals treated with caffeine or MDMA/MDA alone. This hyperthermic response to caffeine and MDMA was not observed with repeated administration, unlike caffeine + MDA, where hyperthermia was obtained over the 4 day treatment period. In addition, 4 weeks after the last treatment, co-administration of caffeine with MDA (but not MDMA) induced a reduction in 5-HT and 5-hydroxyindole acetic acid (5-HIAA) concentrations in frontal cortex (to 61% and 58% of control, respectively), hippocampus (48% and 60%), striatum (79% and 64%) and amygdala (63% and 37%). However, when caffeine (10 mg/kg) and MDMA (2.5 mg/kg) were co-administered four times daily for 2 days to group-housed animals, both hyperthermia and hippocampal 5-HT loss were observed (reduced to 68% of

  12. Overview of Pre-Flight Physical Training, In-Flight Exercise Countermeasures and the Post-Flight Reconditioning Program for International Space Station Astronauts

    NASA Technical Reports Server (NTRS)

    Kerstman, Eric

    2011-01-01

    International Space Station (ISS) astronauts receive supervised physical training pre-flight, utilize exercise countermeasures in-flight, and participate in a structured reconditioning program post-flight. Despite recent advances in exercise hardware and prescribed exercise countermeasures, ISS crewmembers are still found to have variable levels of deconditioning post-flight. This presentation provides an overview of the astronaut medical certification requirements, pre-flight physical training, in-flight exercise countermeasures, and the post-flight reconditioning program. Astronauts must meet medical certification requirements on selection, annually, and prior to ISS missions. In addition, extensive physical fitness testing and standardized medical assessments are performed on long duration crewmembers pre-flight. Limited physical fitness assessments and medical examinations are performed in-flight to develop exercise countermeasure prescriptions, ensure that the crewmembers are physically capable of performing mission tasks, and monitor astronaut health. Upon mission completion, long duration astronauts must re-adapt to the 1 G environment, and be certified as fit to return to space flight training and active duty. A structured, supervised postflight reconditioning program has been developed to prevent injuries, facilitate re-adaptation to the 1 G environment, and subsequently return astronauts to training and space flight. The NASA reconditioning program is implemented by the Astronaut Strength, Conditioning, and Rehabilitation (ASCR) team and supervised by NASA flight surgeons. This program has evolved over the past 10 years of the International Space Station (ISS) program and has been successful in ensuring that long duration astronauts safely re-adapt to the 1 g environment and return to active duty. Lessons learned from this approach to managing deconditioning can be applied to terrestrial medicine and future exploration space flight missions.

  13. Digital adaptive control laws for VTOL aircraft

    NASA Technical Reports Server (NTRS)

    Hartmann, G. L.; Stein, G.

    1979-01-01

    Honeywell has designed a digital self-adaptive flight control system for flight test in the VALT Research Aircraft (a modified CH-47). The final design resulted from a comparison of two different adaptive concepts: one based on explicit parameter estimates from a real-time maximum likelihood estimation algorithm and the other based on an implicit model reference adaptive system. The two designs are compared on the basis of performance and complexity.

  14. Cardiovascular function in space flight

    NASA Technical Reports Server (NTRS)

    Nicogossian, A. E.; Charles, J. B.; Bungo, M. W.; Leach-Huntoon, C. S.; Nicgossian, A. E.

    1991-01-01

    Changes in orthostatic heart rate have been noted universally in Soviet and U.S. crewmembers post space flight. The magnitude of these changes appears to be influenced by mission duration, with increasing orthostatic intolerance for the first 7-10 days of flight and then a partial recovery in the orthostatic heart rate response. Fluid loading has been used as a countermeasure to this postflight orthostatic intolerance. Previous reports have documented the effectiveness of this technique, but it has also been noted that the effectiveness of volume expansion diminishes as flight duration exceeds one week. The response of carotid baroreceptor function was investigated utilizing a commercially available neck collar which could apply positive and negative pressure to effect receptor stimulation. Bedrest studies had validated the usefulness and validity of the device. In these studies it was shown that carotid baroreceptor function curves demonstrated less responsiveness to orthostatic stimulation than control individuals. Twelve Space Shuttle crewmembers were examined pre- and postflight from flights lasting from 4-5 days. Plots of baroreceptor function were constructed and plotted as change in R-R interval vs. carotid distending pressure (an orthostatic stimulus). Typical sigmoidal curves were obtained. Postflight the resting heart rate was higher (smaller R-R interval) and the range of R-R value and the slope of the carotid sigmoidal response were both depressed. These changes were not significant immediately postflight (L + O), but did become significant by the second day postflight (L + 2), and remained suppressed for several days thereafter. It is hypothesized that the early adaptation to space flight involves a central fluid shift during the initial days of flight, but subsequent alterations in neural controlling mechanisms (such as carotid baroreceptor function) contribute to orthostatic intolerance.

  15. MDA-7/IL-24 functions as a tumor suppressor gene in vivo in transgenic mouse models of breast cancer.

    PubMed

    Menezes, Mitchell E; Shen, Xue-Ning; Das, Swadesh K; Emdad, Luni; Guo, Chunqing; Yuan, Fang; Li, You-Jun; Archer, Michael C; Zacksenhaus, Eldad; Windle, Jolene J; Subler, Mark A; Ben-David, Yaacov; Sarkar, Devanand; Wang, Xiang-Yang; Fisher, Paul B

    2015-11-10

    Melanoma differentiation associated gene-7/Interleukin-24 (MDA-7/IL-24) is a novel member of the IL-10 gene family that selectively induces apoptosis and toxic autophagy in a broad spectrum of human cancers, including breast cancer, without harming normal cells or tissues. The ability to investigate the critical events underlying cancer initiation and progression, as well as the capacity to test the efficacy of novel therapeutics, has been significantly advanced by the development of genetically engineered mice (GEMs) that accurately recapitulate specific human cancers. We utilized three transgenic mouse models to better comprehend the in vivo role of MDA-7/IL-24 in breast cancer. Using the MMTV-PyMT spontaneous mammary tumor model, we confirmed that exogenously introducing MDA-7/IL-24 using a Cancer Terminator Virus caused a reduction in tumor burden and also produced an antitumor "bystander" effect. Next we performed xenograft studies in a newly created MMTV-MDA-7 transgenic model that over-expresses MDA-7/IL-24 in the mammary glands during pregnancy and lactation, and found that MDA-7/IL-24 overexpression delayed tumor growth following orthotopic injection of a murine PDX tumor cell line (mPDX) derived from a tumor formed in an MMTV-PyMT mouse. We also crossed the MMTV-MDA-7 line to MMTV-Erbb2 transgenic mice and found that MDA-7/IL-24 overexpression delayed the onset of mammary tumor development in this model of spontaneous mammary tumorigenesis as well. Finally, we assessed the role of MDA-7/IL-24 in immune regulation, which can potentially contribute to tumor suppression in vivo. Our findings provide further direct in vivo evidence for the role of MDA-7/IL-24 in tumor suppression in breast cancer in immune-competent transgenic mice.

  16. Knockout of MDA-9/Syntenin (SDCBP) expression in the microenvironment dampens tumor-supporting inflammation and inhibits melanoma metastasis

    PubMed Central

    Das, Swadesh K.; Guo, Chunqing; Pradhan, Anjan K.; Bhoopathi, Praveen; Talukdar, Sarmistha; Shen, Xue-Ning; Emdad, Luni; Subler, Mark A.; Windle, Jolene J.; Sarkar, Devanand; Wang, Xiang-Yang; Fisher, Paul B.

    2016-01-01

    Cancer development and progression to metastasis is a complex process, which largely depends on bidirectional communication between tumor cells and their microenvironment. Melanoma differentiation associated gene-9 (mda-9, also known as Syntenin-1, SDCBP), a gene first cloned by our group, is robustly expressed in multiple cancers including melanoma and contributes to invasion and metastasis in a tumor cell-intrinsic manner. However, the role of MDA-9/Syntenin in the tumor cell-extrinsic microenvironment remains unclear even though MDA-9/Syntenin is ubiquitously expressed in most organs that are active metastatic sites for melanoma, e.g., lung, lymph node, brain, and liver. In this study, we explored the effect of environmental mda-9/syntenin expression on melanoma growth and metastasis using multiple immunocompetent animal models, syngeneic B16 xenograft and intravenous B16 mouse model and a genetically engineered mouse (GEM) model of melanoma. Host-deficient expression of mda-9/syntenin in mice negatively impacted on subcutaneously implanted B16 tumor growth and lung metastasis. Absence of MDA-9/Syntenin in the lung microenvironment suppressed tumor growth by modulating in situ Interleukin 17A (IL17A) expression and impaired the recruitment of myeloid derived suppressor cells (MDSCs) and Th17 cells as compared to genetically wild type animals. Additionally, loss of mda-9/syntenin expression in a spontaneous melanoma model (melanocyte-specific pten loss and BrafV600E mutation) significantly delayed tumor initiation and suppressed metastasis to the lymph nodes and lungs. The present study highlights a novel role of mda-9/syntenin in tumor-promoting inflammation and immune suppression. These observations along with other documented roles of MDA-9/Syntenin in cancer and metastasis support the potential relevance of MDA-9/Syntenin in the carcinogenic process and as a target for developing improved therapies by using either genetic or pharmacologic approaches to treat

  17. Applying MDA to SDR for Space to Model Real-time Issues

    NASA Technical Reports Server (NTRS)

    Blaser, Tammy M.

    2007-01-01

    NASA space communications systems have the challenge of designing SDRs with highly-constrained Size, Weight and Power (SWaP) resources. A study is being conducted to assess the effectiveness of applying the MDA Platform-Independent Model (PIM) and one or more Platform-Specific Models (PSM) specifically to address NASA space domain real-time issues. This paper will summarize our experiences with applying MDA to SDR for Space to model real-time issues. Real-time issues to be examined, measured, and analyzed are: meeting waveform timing requirements and efficiently applying Real-time Operating System (RTOS) scheduling algorithms, applying safety control measures, and SWaP verification. Real-time waveform algorithms benchmarked with the worst case environment conditions under the heaviest workload will drive the SDR for Space real-time PSM design.

  18. Both RIG-I and MDA5 detect alphavirus replication in concentration-dependent mode.

    PubMed

    Akhrymuk, Ivan; Frolov, Ilya; Frolova, Elena I

    2016-01-01

    Alphaviruses are a family of positive-strand RNA viruses that circulate on all continents between mosquito vectors and vertebrate hosts. Despite a significant public health threat, their biology is not sufficiently investigated, and the mechanisms of alphavirus replication and virus-host interaction are insufficiently understood. In this study, we have applied a variety of experimental systems to further understand the mechanism by which infected cells detect replicating alphaviruses. Our new data strongly suggest that activation of the antiviral response by alphavirus-infected cells is determined by the integrity of viral genes encoding proteins with nuclear functions, and by the presence of two cellular pattern recognition receptors (PRRs), RIG-I and MDA5. No type I IFN response is induced in their absence. The presence of either of these PRRs is sufficient for detecting virus replication. However, type I IFN activation in response to pathogenic alphaviruses depends on the basal levels of RIG-I or MDA5.

  19. Both RIG-I and MDA5 detect alphavirus replication in concentration-dependent mode

    PubMed Central

    Akhrymuk, Ivan; Frolov, Ilya; Frolova, Elena I.

    2015-01-01

    Alphaviruses are a family of positive-strand RNA viruses that circulate on all continents between mosquito vectors and vertebrate hosts. Despite a significant public health threat, their biology is not sufficiently investigated, and the mechanisms of alphavirus replication and virus-host interaction are insufficiently understood. In this study, we have applied a variety of experimental systems to further understand the mechanism by which infected cells detect replicating alphaviruses. Our new data strongly suggest that activation of the antiviral response by alphavirus-infected cells is determined by the integrity of viral genes encoding proteins with nuclear functions, and by the presence of two cellular pattern recognition receptors (PRRs), RIG-I and MDA5. No type I IFN response is induced in their absence. The presence of either of these PRRs is sufficient for detecting virus replication. However, type I IFN activation in response to pathogenic alphaviruses depends on the basal levels of RIG-I or MDA5. PMID:26550947

  20. Chicken cells sense influenza A virus infection through MDA5 and CARDIF signaling involving LGP2.

    PubMed

    Liniger, Matthias; Summerfield, Artur; Zimmer, Gert; McCullough, Kenneth C; Ruggli, Nicolas

    2012-01-01

    Avian influenza viruses (AIV) raise worldwide veterinary and public health concerns due to their potential for zoonotic transmission. While infection with highly pathogenic AIV results in high mortality in chickens, this is not necessarily the case in wild birds and ducks. It is known that innate immune factors can contribute to the outcome of infection. In this context, retinoic acid-inducible gene I (RIG-I) is the main cytosolic pattern recognition receptor known for detecting influenza A virus infection in mammalian cells. Chickens, unlike ducks, lack RIG-I, yet chicken cells do produce type I interferon (IFN) in response to AIV infection. Consequently, we sought to identify the cytosolic recognition elements in chicken cells. Chicken mRNA encoding the putative chicken analogs of CARDIF and LGP2 (chCARDIF and chLGP2, respectively) were identified. HT7-tagged chCARDIF was observed to associate with mitochondria in chicken DF-1 fibroblasts. The exogenous expression of chCARDIF, as well as of the caspase activation and recruitment domains (CARDs) of the chicken melanoma differentiation-associated protein 5 (chMDA5), strongly activated the chicken IFN-β (chIFN-β) promoter. The silencing of chMDA5, chCARDIF, and chIRF3 reduced chIFN-β levels induced by AIV, indicating their involvement in AIV sensing. As with mammalian cells, chLGP2 had opposing effects. While overexpression decreased the activation of the chIFN-β promoter, the silencing of endogenous chLGP2 reduced chIFN-β induced by AIV. We finally demonstrate that the chMDA5 signaling pathway is inhibited by the viral nonstructural protein 1. In conclusion, chicken cells, including DF-1 fibroblasts and HD-11 macrophage-like cells, employ chMDA5 for sensing AIV.

  1. Cardiovascular adaptation to spaceflight

    NASA Technical Reports Server (NTRS)

    Hargens, A. R.; Watenpaugh, D. E.

    1996-01-01

    This article reviews recent flight and ground-based studies of cardiovascular adaptation to spaceflight. Prominent features of microgravity exposure include loss of gravitational pressures, relatively low venous pressures, headward fluid shifts, plasma volume loss, and postflight orthostatic intolerance and reduced exercise capacity. Many of these short-term responses to microgravity extend themselves during long-duration microgravity exposure and may be explained by altered pressures (blood and tissue) and fluid balance in local tissues nourished by the cardiovascular system. In this regard, it is particularly noteworthy that tissues of the lower body (e.g., foot) are well adapted to local hypertension on Earth, whereas tissues of the upper body (e.g., head) are not as well adapted to increase in local blood pressure. For these and other reasons, countermeasures for long-duration flight should include reestablishment of higher, Earth-like blood pressures in the lower body.

  2. Toxicity of MDA (3,4-methylenedioxyamphetamine) considered for relevance to hazards of MDMA (Ecstasy) abuse.

    PubMed

    Davis, W M; Hatoum, H T; Waters, I W

    1987-01-01

    Despite a paucity of data on its animal pharmacology and toxicology, MDMA [Ecstasy, XTC, ADAM; (+/-)-3,4-methylenedioxymethamphetamine] was introduced as an "underground" (FDA-unapproved) adjunct to psychotherapy in the late 1970's and early 1980's, in addition to its use as a recreational drug. Analysis of the limited experimental literature indicates that LD50's for MDMA in five species by several routes of administration tend to predict a significant human toxicity. MDMA was either equally toxic or slightly to moderately less toxic than its close congener, MDA, (+/-)-3,4-methylenedioxyamphetamine. It is suggested that extrapolation of the pharmacologic/toxicologic data available for MDA to MDMA should be assumed to be valid until disproven. Recently published canine data describe physiologic disturbances caused by acute overdosage of MDA, and also indicate the utility of chlorpromazine as an antidote preventing fatalities associated with severe hyperthermia, lactacidemia, hypertension and tachycardia. The toxicology of MDMA warrants further direct study in view of its continuing illegal distribution.

  3. Cytotoxicity of Biologically Synthesized Silver Nanoparticles in MDA-MB-231 Human Breast Cancer Cells

    PubMed Central

    Gurunathan, Sangiliyandi; Han, Jae Woong; Eppakayala, Vasuki; Jeyaraj, Muniyandi; Kim, Jin-Hoi

    2013-01-01

    Silver nanoparticles (AgNPs) have been used as an antimicrobial and disinfectant agents. However, there is limited information about antitumor potential. Therefore, this study focused on determining cytotoxic effects of AgNPs on MDA-MB-231 breast cancer cells and its mechanism of cell death. Herein, we developed a green method for synthesis of AgNPs using culture supernatant of Bacillus funiculus, and synthesized AgNPs were characterized by various analytical techniques such as UV-visible spectrophotometer, particle size analyzer, and transmission electron microscopy (TEM). The toxicity was evaluated using cell viability, metabolic activity, and oxidative stress. MDA-MB-231 breast cancer cells were treated with various concentrations of AgNPs (5 to 25 μg/mL) for 24 h. We found that AgNPs inhibited the growth in a dose-dependent manner using MTT assay. AgNPs showed dose-dependent cytotoxicity against MDA-MB-231 cells through activation of the lactate dehydrogenase (LDH), caspase-3, reactive oxygen species (ROS) generation, eventually leading to induction of apoptosis which was further confirmed through resulting nuclear fragmentation. The present results showed that AgNPs might be a potential alternative agent for human breast cancer therapy. PMID:23936814

  4. Obesity And Laboratory Diets Affects Tissue Malondialdehyde (MDA) Levels In Obese Rats

    NASA Astrophysics Data System (ADS)

    Chowdhury, Parimal; Scott, Joseph; Holley, Andy; Hakkak, Reza

    2010-04-01

    This study was conducted to investigate the interaction of obesity and laboratory diets on tissue malondialdehyde levels in rats. Female Zucker obese and lean rats were maintained on either regular grain-based diet or purified casein diet for two weeks, orally gavaged at day 50 with 65 mg/kg DMBA and sacrificed 24 hrs later. Malondialdehyde (MDA) levels were measured in blood and harvested tissues. Data were recorded as mean ± SEM and analyzed statistically. Results show that the obese group on purified casein diet had reduction of MDA levels in the brain, duodenum, liver, lung and kidney tissues as compared to lean group, p <0.05. Obese group on grain-based diet showed significant increase in MDA levels only in the duodenum, p <0.05. We conclude that dietary intervention differentially affects the oxidative markers in obese rats. It appears that purified casein diets were more effective than grain-based diet in reduction of oxidative stress in obese rats.

  5. IRAC Full-Scale Flight Testbed Capabilities

    NASA Technical Reports Server (NTRS)

    Lee, James A.; Pahle, Joseph; Cogan, Bruce R.; Hanson, Curtis E.; Bosworth, John T.

    2009-01-01

    Overview: Provide validation of adaptive control law concepts through full scale flight evaluation in a representative avionics architecture. Develop an understanding of aircraft dynamics of current vehicles in damaged and upset conditions Real-world conditions include: a) Turbulence, sensor noise, feedback biases; and b) Coupling between pilot and adaptive system. Simulated damage includes 1) "B" matrix (surface) failures; and 2) "A" matrix failures. Evaluate robustness of control systems to anticipated and unanticipated failures.

  6. Link Dependent Adaptive Radio Simulation

    DTIC Science & Technology

    2014-06-01

    14. ABSTRACT This paper shows the optimized Link Dependent Adaptive Radio (LDAR) using the variable QAM OFDM modulation size which adapts to channel...bit error rate (BER), Orthogonal Frequency Division Multiplexing ( OFDM ) 16. SECURITY CLASSIFICATION OF: Unclassified 17. LIMITATION OF ABSTRACT...using the variable QAM OFDM modulation size which adapts to channel conditions. The LDAR enhanced performance is illustrated by use of a flight path

  7. Regionalization of Agricultural Management by Using the Multi-Data Approach (mda)

    NASA Astrophysics Data System (ADS)

    Bareth, G.; Waldhoff, G.

    2012-07-01

    Regional process-based (agro-)ecosystem modelling depends mainly on data availability of land use, weather, soil, and agricultural management. While land use, weather, and soil data are available from official sources or can be captured with monitoring systems, management data are usually derived from official statistics for administrative units. For numerous spatial modeling approaches, these data are not satisfying. Especially for process-based agro-ecosystem modeling on regional scales, spatially disaggregated and land use dependent information on agricultural management is a must. Information about date of sowing, dates of fertilization, dates of weeding etc. are required as input parameters by such models. These models consider nitrogen (N)- and carbon (C)-matter fluxes but essential amounts of N-/C-input and N-/C-output are determined by crop management. Therefore, in this contribution a RS- and GIS-based approach for regional generation of management data is introduced. The approach is based on the Multi-data Approach (MDA) for enhanced land use/land cover mapping. The MDA is a combined RS and GIS approach. The retrieved information from multitemporal and multisensoral remote sensing analysis is integrated into official land use data to enhance both the information level of existing land use data and the quality of the land use classification. The workflow of the MDA to generate enhanced land use and land cover data consists basically of two components: (a) the methods and data of the remote sensing analysis and (b) the methods and data of the GIS analysis. The MDA results in disaggregated land use data which can be used to link crop management information about the major crops and especially crop rotations like date of sowing, fertilization, irrigation, harvest etc. to the derived land use classes. Consequently, depending on the land use, a distinct management is given in a spatial context on regional scale. In this contribution, three case studies of

  8. F-111 TACT Flight Over the Mojave Desert

    NASA Video Gallery

    Transonic aircraft technology (TACT/F-111A) added an highly efficient supercritical wing and later the third phase applied advanced wing (Mission Adaptive Wing-MAW) flight control technologies and ...

  9. Flight Test Engineering

    NASA Technical Reports Server (NTRS)

    Pavlock, Kate Maureen

    2013-01-01

    Although the scope of flight test engineering efforts may vary among organizations, all point to a common theme: flight test engineering is an interdisciplinary effort to test an asset in its operational flight environment. Upfront planning where design, implementation, and test efforts are clearly aligned with the flight test objective are keys to success. This chapter provides a top level perspective of flight test engineering for the non-expert. Additional research and reading on the topic is encouraged to develop a deeper understanding of specific considerations involved in each phase of flight test engineering.

  10. Innate immunity to RNA virus is regulated by temporal and reversible sumoylation of RIG-I and MDA5.

    PubMed

    Hu, Ming-Ming; Liao, Chen-Yang; Yang, Qing; Xie, Xue-Qin; Shu, Hong-Bing

    2017-04-03

    Sensing of viral RNA by the cytosolic receptors RIG-I and melanoma differentiation-associated gene 5 (MDA5) leads to innate antiviral response. How RIG-I and MDA5 are dynamically regulated in innate antiviral response is not well understood. Here, we show that TRIM38 positively regulates MDA5- and RIG-I-mediated induction of downstream genes and acts as a SUMO E3 ligase for their dynamic sumoylation at K43/K865 and K96/K888, respectively, before and after viral infection. The sumoylation of MDA5 and RIG-I suppresses their K48-linked polyubiquitination and degradation in uninfected or early-infected cells. Sumoylation of the caspase recruitment domains of MDA5 and RIG-I is also required for their dephosphorylation by PP1 and activation upon viral infection. At the late phase of viral infection, both MDA5 and RIG-I are desumoylated by SENP2, resulting in their K48-linked polyubiquitination and degradation. These findings suggest that dynamic sumoylation and desumoylation of MDA5 and RIG-I modulate efficient innate immunity to RNA virus and its timely termination.

  11. Melanoma differentiation associated gene-7/interleukin-24 (mda-7/IL-24): Novel gene therapeutic for metastatic melanoma

    SciTech Connect

    Fisher, Paul B. Sarkar, Devanand; Lebedeva, Irina V.; Emdad, Luni; Gupta, Pankaj; Sauane, Moira; Su Zaozhong; Grant, Steven; Dent, Paul; Curiel, David T.; Senzer, Neil; Nemunaitis, John

    2007-11-01

    A potentially less toxic approach for cancer therapy comprises induction of tumor cells to lose growth potential irreversibly and terminally differentiate. Combining this scheme termed 'differentiation therapy of cancer' with subtraction hybridization to human melanoma cells resulted in the cloning of melanoma differentiation associated (mda) genes displaying elevated expression as a consequence of induction of terminal differentiation. One originally novel gene, mda-7, was found to display elevated expression in normal melanocytes and nevi with progressive loss of expression as a consequence of melanoma development and progression to metastasis. Based on structure, biochemical properties and chromosomal location, mda-7 has now been reclassified as interleukin (IL)-24, a member of the expanding IL-10 family of cytokines. In vitro cell culture and in vivo animal studies indicate that mda-7/IL-24 selectively induces programmed cell death (apoptosis) in multiple human cancers (including melanomas), without harming normal cells, and promotes profound anti-tumor activity in nude mice containing human tumor xenografts. Based on these remarkable properties, a Phase I clinical trial was conducted to test the safety of administration of mda-7/IL-24 by a replication incompetent adenovirus (Ad.mda-7; INGN 241) in patients with advanced solid cancers including melanoma. mda-7/IL-24 was found to be safe and to promote significant clinical activity, particularly in the context of patients with metastatic melanoma. These results provide an impetus for further clinical studies and document a central paradigm of cancer therapy, namely translation of basic science from the 'bench to the bedside.'.

  12. MDA-9/Syntenin-Slug transcriptional complex promote epithelial-mesenchymal transition and invasion/metastasis in lung adenocarcinoma

    PubMed Central

    Wang, Lu-Kai; Pan, Szu-Hua; Chang, Yih-Leong; Hung, Pei-Fang; Kao, Shih-Han; Wang, Wen-Lung; Lin, Ching-Wen; Yang, Shuenn-Chen; Liang, Chen-Hsien; Wu, Chen-Tu; Hsiao, Tzu-Hung

    2016-01-01

    Melanoma differentiation-associated gene-9 (MDA-9)/Syntenin is a novel therapeutic target because it plays critical roles in cancer progression and exosome biogenesis. Here we show that Slug, a key epithelial-mesenchymal-transition (EMT) regulator, is a MDA-9/Syntenin downstream target. Mitogen EGF stimulation increases Slug expression and MDA-9/Syntenin nuclear translocation. MDA-9/Syntenin uses its PDZ1 domain to bind with Slug, and this interaction further leads to HDAC1 recruitment, up-regulation of Slug transcriptional repressor activity, enhanced Slug-mediated EMT, and promotion of cancer invasion and metastasis. The PDZ domains and nuclear localization of MDA-9/Syntenin are both required for promoting Slug-mediated cancer invasion. Clinically, patients with high MDA-9/Syntenin and high Slug expressions were associated with poor overall survival compared to those with low expression in lung adenocarcinomas. Our findings provide evidence that MDA-9/Syntenin acts as a pivotal adaptor of Slug and it transcriptionally enhances Slug-mediated EMT to promote cancer invasion and metastasis. PMID:26561205

  13. MDA7: a novel selective agonist for CB2 receptors that prevents allodynia in rat neuropathic pain models

    PubMed Central

    Naguib, M; Diaz, P; Xu, J J; Astruc-Diaz, F; Craig, S; Vivas-Mejia, P; Brown, D L

    2008-01-01

    Background and purpose: There is growing interest in using cannabinoid type 2 (CB2) receptor agonists for the treatment of neuropathic pain. In this report, we describe the pharmacological characteristics of MDA7 (1-[(3-benzyl-3-methyl-2,3-dihydro-1-benzofuran-6-yl)carbonyl]piperidine), a novel CB2 receptor agonist. Experimental approach: We characterized the pharmacological profile of MDA7 by using radioligand-binding assays and in vitro functional assays at human cannabinoid type 1 (CB1) and CB2 receptors. In vitro functional assays were performed at rat CB1 and CB2 receptors. The effects of MDA7 in reversing neuropathic pain were assessed in spinal nerve ligation and paclitaxel-induced neuropathy models in rats. Key results: MDA7 exhibited selectivity and agonist affinity at human and rat CB2 receptors. MDA7 treatment attenuated tactile allodynia produced by spinal nerve ligation or by paclitaxel in a dose-related manner. These effects were selectively antagonized by a CB2 receptor antagonist but not by CB1 or opioid receptor antagonists. MDA7 did not affect rat locomotor activity. Conclusion and implications: MDA7, a novel selective CB2 agonist, was effective in suppressing neuropathic nociception in two rat models without affecting locomotor behaviour. These results confirm the potential for CB2 agonists in the treatment of neuropathic pain. PMID:18846037

  14. The Implications of Detrital Zircon Maximum Depositional Age (MDA) from Large Sample Datasets (n>500)

    NASA Astrophysics Data System (ADS)

    Coutts, D. S.; Matthews, W.; Guest, B.; Hubbard, S. M.

    2015-12-01

    The youngest sub-population of a detrital zircon geochronological dataset is routinely used to approximate the age of deposition for a sample. The ages represent a maximum depositional age (MDA) because the detrital zircons analyzed crystallized at depth, in a magma chamber prior to being exposed at the surface through erosion or volcanic eruption. Dickinson and Gehrels (2009) demonstrate four methods of calculating the MDA of a zircon population using U-Pb ages, which are assessed in this study. Previous MDA studies used relatively small datasets (n<100), reducing the likelihood of finding the youngest population in a sample. We consider large-n datasets (n>500), which have a greater likelihood of capturing a significant proportion of the youngest population and therefore have the potential to improve the accuracy of a calculated MDA. We assess the effects of sample size and MDA calculation methods using a numerical model consisting of a simulated population of detrital zircon grains with known ages. Using our population of 25048 simulated grains, we ran repeated trials of varying sample sizes (n=50, 100, 300, 500, 700, 1000, 1500) to compare the output of MDA calculation techniques. As sample size increases the youngest sub-population of zircons is better defined, and the MDA decreases and becomes more precise. As a further test, model results are compared to U-Pb data (n=695) from a sample of the Maasrichtian-Paleocene Gabriola Formation (Nanaimo Group, B.C., Canada). Similar trials of varying sample sizes show the same decrease in MDA. Biostratigraphic analysis assigned the formation to the Maastrichtian (72.1 - 66.0 Ma), however, our results indicate that deposition took place in the Danian (66.0 - 61.6 Ma). This result has implications for the timing of forearc basin fill, and more broadly, the evolution of the Western North American Cordillera. MDA methods on large-n datasets can be used to hone stratigraphic correlations and calculate sediment accumulation

  15. In-flight aeroelastic measurement technique development

    NASA Astrophysics Data System (ADS)

    Burner, Alpheus W.; Lokos, William A.; Barrows, Danny A.

    2003-11-01

    The initial concept and development of a low-cost, adaptable method for the measurement of static and dynamic aeroelastic deformation of aircraft during flight testing is presented. The method is adapted from a proven technique used in wind tunnel testing to measure model deformation, often referred to as the videogrammetric model deformation (or VMD) technique. The requirements for in-flight measurements are compared and contrasted with those for wind tunnel testing. The methodology for the proposed measurements and differences compared with that used for wind tunnel testing is given. Several error sources and their effects are identified. Measurement examples using the new technique, including change in wing twist and deflection as a function of time, from an F/A-18 research aircraft at NASA's Dryden Flight Research Center are presented.

  16. A novel dermato-pulmonary syndrome associated with MDA-5 antibodies: report of 2 cases and review of the literature.

    PubMed

    Chaisson, Neal F; Paik, Julie; Orbai, Ana-Maria; Casciola-Rosen, Livia; Fiorentino, David; Danoff, Sonye; Rosen, Antony

    2012-07-01

    Melanoma differentiation-associated protein 5 (MDA-5) is a novel autoantibody frequently characterized by interstitial lung disease and a distinct cutaneous phenotype with palmar papules, ulceration, and rash. Virtually all patients have underlying dermatomyositis, but many lack the characteristic clinical myopathy associated with it. In the setting of amyopathic disease, the absence of clinically available biomarkers or clear pathologic diagnosis can complicate effective prognostic and therapeutic intervention. Until recently the presence of MDA-5 antibody associated dermato-pulmonary syndrome was described only in Asian populations. We present 2 cases of MDA-5-associated dermato-pulmonary syndrome and provide a comprehensive review of available literature.

  17. Planning Flight Paths of Autonomous Aerobots

    NASA Technical Reports Server (NTRS)

    Kulczycki, Eric; Elfes, Alberto; Sharma, Shivanjli

    2009-01-01

    Algorithms for planning flight paths of autonomous aerobots (robotic blimps) to be deployed in scientific exploration of remote planets are undergoing development. These algorithms are also adaptable to terrestrial applications involving robotic submarines as well as aerobots and other autonomous aircraft used to acquire scientific data or to perform surveying or monitoring functions.

  18. Operational efficiency: Automatic ascent flight design

    NASA Technical Reports Server (NTRS)

    1990-01-01

    Major objectives, milestones, key contacts, major accomplishments, technology issues, and candidate programs of the automatic ascent flight design are outlined. Topics discussed include: advanced avionics concepts; advanced training concepts; telerobotics/telepresence; integrated command and control; advanced software integration; atmospheric adaptive guidance; and health status and monitoring concept. This presentation is represented by viewgraphs only.

  19. Integrating Space Flight Resource Management Skills into Technical Lessons for International Space Station Flight Controller Training

    NASA Technical Reports Server (NTRS)

    Baldwin, Evelyn

    2008-01-01

    The Johnson Space Center s (JSC) International Space Station (ISS) Space Flight Resource Management (SFRM) training program is designed to teach the team skills required to be an effective flight controller. It was adapted from the SFRM training given to Shuttle flight controllers to fit the needs of a "24 hours a day/365 days a year" flight controller. More recently, the length reduction of technical training flows for ISS flight controllers impacted the number of opportunities for fully integrated team scenario based training, where most SFRM training occurred. Thus, the ISS SFRM training program is evolving yet again, using a new approach of teaching and evaluating SFRM alongside of technical materials. Because there are very few models in other industries that have successfully tied team and technical skills together, challenges are arising. Despite this, the Mission Operations Directorate of NASA s JSC is committed to implementing this integrated training approach because of the anticipated benefits.

  20. 'Mighty Eagle' Takes Flight

    NASA Video Gallery

    The "Mighty Eagle," a NASA robotic prototype lander, had a successful first untethered flight Aug. 8 at the Marshall Center. During the 34-second flight, the Mighty Eagle soared and hovered at 30 f...

  1. Autonomous Soaring Flight Results

    NASA Technical Reports Server (NTRS)

    Allen, Michael J.

    2006-01-01

    A viewgraph presentation on autonomous soaring flight results for Unmanned Aerial Vehicles (UAV)'s is shown. The topics include: 1) Background; 2) Thermal Soaring Flight Results; 3) Autonomous Dolphin Soaring; and 4) Future Plans.

  2. Who dares to join a parabolic flight?

    NASA Astrophysics Data System (ADS)

    Montag, Christian; Zander, Tina; Schneider, Stefan

    2016-12-01

    Parabolic flights represent an important tool in space research to investigate zero gravity on airplanes. Research on these flights often target psychological and biological processes in humans to investigate if and how we can adapt to this unique environment. This research is costly, hard to conduct and clearly heavily relies on humans participating in experiments in this (unnatural) situation. The present study investigated N =66 participants and N =66 matched control persons to study if participants in such experimental flights differ in terms of their personality traits from non-parabonauts. The main finding of this study demonstrates that parabonauts score significantly lower on harm avoidance, a trait closely linked to being anxious. As anxious humans differ from non-anxious humans in their biology, the present observations need to be taken into account when aiming at the generalizability of psychobiological research findings conducted in zero gravity on parabolic flights.

  3. Liability and Insurance for Suborbital Flights

    NASA Astrophysics Data System (ADS)

    Masson-Zwaan, T.

    2012-01-01

    This paper analyzes and compares liability and liability insurance in the fields of aviation and spaceflight in order to propose solutions for a liability regime and insurance options for suborbital flights. Suborbital flights can be said to take place in the grey zone between air and space, between air law and space law, as well as between aviation insurance and space insurance. In terms of liability, the paper discusses air law and space law provisions in the fields of second and third party liability for damage to passengers and 'innocent bystanders' respectively, touching upon international treaties, national law and EU law, and on insurance to cover those risks. Although the insurance market is currently not ready to provide tailor-made products for operators of suborbital flights, it is expected to adapt rapidly once such flights will become reality. A hybrid approach will provide the best solution in the medium term.

  4. Endocrine responses to space flights.

    PubMed

    Macho, L; Kvetnansky, R; Fickova, M; Kolena, J; Knopp, J; Tigranian, R A; Popova, I A; Grogoriev, A I

    2001-07-01

    Simultaneously with human space flights several series of observations were performed by using experimental animals--mainly rats--exposed to space flights on board of special satellites BION-COSMOS or in Shuttle Transportation Systems (STS). The aims of these experiments were to study in more details: the mechanisms of the changes in bones and skeletal muscle, the alterations of the function of immune system, the radiation effects on organism, the mechanism of the changes of endocrine functions, the evaluation of the role of hormones in alteration of metabolic processes in organism. The advantages of these animal experiments were the possibilities to analyze not only the plasma samples, but it was possible to obtain samples of organs or tissues: for morphological and biochemical analysis for studies of the changes in enzyme activities and in gene expressions, for measurement of metabolic processes and for investigation of the hormone production in endocrine glands and estimation of the response of tissues to hormones. It was also possible to compare the endocrine response to spaceflight and to other stress stimuli. These animal studies are interesting for verification of some hypothesis in the mechanism of adaptation of human organism to the changes of gravity. The disadvantage was, however, that the animals in almost all experiments could be examined only after space flight. The actual inflight changes were investigated only in two SLS flights. In this short review it is not possible to evaluate all hormonal data available on the response of endocrine system to the conditions of space flights. Therefore we will concentrate on the response of pituitary adrenocortical system, pituitary thyroid and pituitary gonadal functions.

  5. Life-sciences research opportunities in commercial suborbital space flight

    NASA Astrophysics Data System (ADS)

    Shelhamer, Mark

    2014-11-01

    Commercial suborbital space flights will reach altitudes above 100 km, with 3-5 min of weightlessness bracketed by high-g launch and landing phases. The proposed frequency of these flights, and the large passenger population, present interesting opportunities for researchers in the life sciences. The characteristics of suborbital flight are between those of parabolic and orbital flights, opening up new scientific possibilities and easing the burden for obtaining access to 0g. There are several areas where these flights might be used for research in the life sciences: (1) operational research: preparation for “real” space flight, such as rehearsal of medical procedures, (2) applied research-to answer questions relevant to long-term space flight; (3) passenger health and safety-effects on passengers, relevant to screening and training; (4) basic research in physiological mechanisms-to address issues of fundamental science. We describe possible projects in each of these categories. One in particular spans several areas. Based on the anticipated suborbital flight profiles, observations from parabolic flight, and the wide range of fitness and experience levels of suborbital passengers, sensorimotor disturbances such as motion sickness and disorientation are major concerns. Protocols for pre-flight adaptation of sensorimotor responses might help to alleviate some of these problems, based on results from research in the initial flights. This would improve the passenger experience and add to the knowledge base relevant to space flight more generally.

  6. Surface tension dominates insect flight on fluid interfaces

    PubMed Central

    Mukundarajan, Haripriya; Bardon, Thibaut C.; Kim, Dong Hyun; Prakash, Manu

    2016-01-01

    ABSTRACT Flight on the 2D air–water interface, with body weight supported by surface tension, is a unique locomotion strategy well adapted for the environmental niche on the surface of water. Although previously described in aquatic insects like stoneflies, the biomechanics of interfacial flight has never been analysed. Here, we report interfacial flight as an adapted behaviour in waterlily beetles (Galerucella nymphaeae) which are also dexterous airborne fliers. We present the first quantitative biomechanical model of interfacial flight in insects, uncovering an intricate interplay of capillary, aerodynamic and neuromuscular forces. We show that waterlily beetles use their tarsal claws to attach themselves to the interface, via a fluid contact line pinned at the claw. We investigate the kinematics of interfacial flight trajectories using high-speed imaging and construct a mathematical model describing the flight dynamics. Our results show that non-linear surface tension forces make interfacial flight energetically expensive compared with airborne flight at the relatively high speeds characteristic of waterlily beetles, and cause chaotic dynamics to arise naturally in these regimes. We identify the crucial roles of capillary–gravity wave drag and oscillatory surface tension forces which dominate interfacial flight, showing that the air–water interface presents a radically modified force landscape for flapping wing flight compared with air. PMID:26936640

  7. In Flight, Online

    ERIC Educational Resources Information Center

    Lucking, Robert A.; Wighting, Mervyn J.; Christmann, Edwin P.

    2005-01-01

    The concept of flight for human beings has always been closely tied to imagination. To fly like a bird requires a mind that also soars. Therefore, good teachers who want to teach the scientific principles of flight recognize that it is helpful to share stories of their search for the keys to flight. The authors share some of these with the reader,…

  8. Direct Type I IFN but Not MDA5/TLR3 Activation of Dendritic Cells Is Required for Maturation and Metabolic Shift to Glycolysis after Poly IC Stimulation

    PubMed Central

    Haddad, Elias; Wood, Elizabeth G.; Longhi, M. Paula

    2014-01-01

    Type I interferons (IFNs) play an important role in direct antiviral defense as well as linking the innate and adaptive immune responses. On dendritic cells (DCs), IFNs facilitate their activation and contribute to CD8+ and CD4+ T cell priming. However, the precise molecular mechanism by which IFNs regulate maturation and immunogenicity of DCs in vivo has not been studied in depth. Here we show that, after in vivo stimulation with the TLR ligand poly IC, IFNs dominate transcriptional changes in DCs. In contrast to direct TLR3/mda5 signaling, IFNs are required for upregulation of all pathways associated with DC immunogenicity. In addition, metabolic pathways, particularly the switch from oxidative phosphorylation to glycolysis, are also regulated by IFNs and required for DC maturation. These data provide evidence for a metabolic reprogramming concomitant with DC maturation and offer a novel mechanism by which IFNs modulate DC maturation. PMID:24409099

  9. Changes of anti-oxidative enzymes and MDA content under soil water deficits among 10 wheat (Triticum aestivum L.) genotypes at maturation stage.

    PubMed

    HongBo, Shao; ZongSuo, Liang; MingAn, Shao

    2005-09-25

    Drought is a world-spread problem seriously influencing grain production and quality, the loss of which is the total for other natural disasters, with increasing global climate change making the situation more serious. Wheat is the staple food for more than 35% of world population, so wheat anti-drought physiology study is of importance to wheat production and biological breeding for the sake of coping with abiotic and biotic conditions. Much research is involved in this hot topic, but the pace of progress is not so large because of drought resistance being a multiple-gene-control quantitative character and wheat genome being larger (16,000Mb). On the other hand, stress adaptive mechanisms are quite different, with stress degree, time course, materials, soil quality status and experimental plots, thus increasing the complexity of the issue in question. Additionally, a little study is related to the whole life circle of wheat, which cannot provide a comprehensive understanding of its anti-drought machinery. We selected 10 kinds of wheat genotypes as materials, which have potential to be applied in practice, and measured change of relative physiological indices through wheat whole growing-developmental circle (i.e. seedling, tillering and maturing). Here, we reported the anti-oxidative results of maturation stage (the results of seedling and tillering stage have been published) in terms of activities of POD, SOD, CAT and MDA content as follows: (1) 10 wheat genotypes can be grouped into three kinds (A-C, respectively) according to their changing trend of the measured indices; (2) A group performed better resistance drought under the condition of treatment level 1 (appropriate level), whose activities of anti-oxidative enzymes (POD, SOD, CAT) were higher and MDA lower; (3) B group exhibited stronger anti-drought under treatment level 2 (light-stress level), whose activities of anti-oxidative enzymes were higher and MDA lower; (4) C group expressed anti-drought to some

  10. Glutamine deprivation sensitizes human breast cancer MDA-MB-231 cells to TRIAL-mediated apoptosis.

    PubMed

    Dilshara, Matharage Gayani; Jeong, Jin-Woo; Prasad Tharanga Jayasooriya, Rajapaksha Gedara; Neelaka Molagoda, Ilandarage Menu; Lee, Seungheon; Park, Sang Rul; Choi, Yung Hyun; Kim, Gi-Young

    2017-04-01

    Tumor cell metabolism is a promising target for various cancer treatments. Apart from aerobic glycolysis, cancer cell growth is dependent on glutamine (Gln) supply, leading to their survival and differentiation. Therefore, we examined whether treatment with TNF-related apoptosis-inducing ligand (TRAIL) sensitizes MDA-MB-231 cells to apoptosis under Gln deprivation condition (TRAIL/Gln deprivation). Gln deprivation decreased cell proliferation as expected, but did not induce remarkable cell death. TRAIL/Gln deprivation, however, significantly increased growth inhibition and morphological shrinkage of MDA-MB-231 cells compared to those induced by treatment with either Gln deprivation or TRAIL alone. Moreover, TRAIL/Gln deprivation upregulated the apoptotic sub-G1 phase accompanied with a remarkable decrease of pro-caspase-3, pro-caspase-9, and anti-apoptotic xIAP, and Bcl-2. Increased cleavage of PARP and pro-apoptotic Bid protein expression suggests that TRAIL/Gln deprivation triggers mitochondrion-mediated apoptosis in MDA-MB-231 cells. Additionally, TRAIL/Gln deprivation upregulated the expression of endoplasmic reticulum (ER) stress markers such as ATF4 and phosphorylated eIF2α, thereby enhancing the C/EBP homologous protein (CHOP) protein level. Transient knockdown of CHOP partically reversed TRAIL/Gln deprivation-mediated apoptosis. Accordingly, TRAIL/Gln deprivation enhanced the expression of death receptor 5 (DR5) and transient knockdown of DR5 completely restored TRAIL/Gln deprivation-mediated apoptosis. Taken together, our results suggest that Gln deprivation conditions can be used for the development of new therapies for TRAIL-resistant cancers.

  11. Baseline geochemistry of soil and bedrock Tshirege Member of the Bandelier Tuff at MDA-P

    SciTech Connect

    Warren, R.G.; McDonald, E.V.; Ryti, R.T.

    1997-08-01

    This report provides baseline geochemistry for soils (including fill), and for bedrock within three specific areas that are planned for use in the remediation of Material Disposal Area P (MDA-P) at Technical Area 16 (TA-16). The baseline chemistry includes leachable element concentrations for both soils and bedrock and total element concentrations for all soil samples and for two selected bedrock samples. MDA-P operated from the early 1950s to 1984 as a landfill for rubble and debris generated by the burning of high explosives (HE) at the TA-16 Burning Ground, HE-contaminated equipment and material, barium nitrate sand, building materials, and trash. The aim of this report is to establish causes for recognizable chemical differences between the background and baseline data sets. In many cases, the authors conclude that recognizable differences represent natural enrichments. In other cases, differences are best attributed to analytical problems. But most importantly, the comparison of background and baseline geochemistry demonstrates significant contamination for several elements not only at the two remedial sites near the TA-16 Burning Ground, but also within the entire region of the background study. This contamination is highly localized very near to the surface in soil and fill, and probably also in bedrock; consequently, upper tolerance limits (UTLs) calculated as upper 95% confidence limits of the 95th percentile are of little value and thus are not provided. This report instead provides basic statistical summaries and graphical comparisons for background and baseline samples to guide strategies for remediation of the three sites to be used in the restoration of MDA-P.

  12. Adaptive Controller Effects on Pilot Behavior

    NASA Technical Reports Server (NTRS)

    Trujillo, Anna C.; Gregory, Irene M.; Hempley, Lucas E.

    2014-01-01

    Adaptive control provides robustness and resilience for highly uncertain, and potentially unpredictable, flight dynamics characteristic. Some of the recent flight experiences of pilot-in-the-loop with an adaptive controller have exhibited unpredicted interactions. In retrospect, this is not surprising once it is realized that there are now two adaptive controllers interacting, the software adaptive control system and the pilot. An experiment was conducted to categorize these interactions on the pilot with an adaptive controller during control surface failures. One of the objectives of this experiment was to determine how the adaptation time of the controller affects pilots. The pitch and roll errors, and stick input increased for increasing adaptation time and during the segment when the adaptive controller was adapting. Not surprisingly, altitude, cross track and angle deviations, and vertical velocity also increase during the failure and then slowly return to pre-failure levels. Subjects may change their behavior even as an adaptive controller is adapting with additional stick inputs. Therefore, the adaptive controller should adapt as fast as possible to minimize flight track errors. This will minimize undesirable interactions between the pilot and the adaptive controller and maintain maneuvering precision.

  13. Synergistic action of cisplatin and echistatin in MDA-MB-231 breast cancer cells.

    PubMed

    Czarnomysy, Robert; Surażyński, Arkadiusz; Popławska, Bożena; Rysiak, Edyta; Pawłowska, Natalia; Czajkowska, Anna; Bielawski, Krzysztof; Bielawska, Anna

    2017-03-01

    The aim of our study was to determine whether the use of cisplatin in the presence echistatin in MDA-MB-231 breast cancer cells leads to a reduction of toxic effects associated with the use of cisplatin. The expression of β1-integrin and insulin-like growth factor 1 receptor (IGF-IR), signaling pathway protein expression: protein kinase B (AKT), mitogen-activated protein kinases (ERK1/ERK2), nuclear factor kappa B (NFκB), and caspase-3 and -9 activity was measured after 24 h of incubation with tested compounds to explain detailed molecular mechanism of induction of apoptosis. The viability of MDA-MB-231 breast cancer cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Annexin V-FITC/propidium iodide staining assay was performed to detect the induction of apoptosis. Inhibition DNA biosynthesis was determined by [(3)H]thymidine incorporation into DNA. The expression of of β1-integrin, IGF-IR, AKT, ERK1/ERK2, NFκB, caspase-3 and -9 was evaluated using Western blot. The results suggest that treatment of MDA-MB-231 breast cancer cells for 24 h cisplatin plus echistatin severely inhibits cell growth and activates apoptosis by upregulation of caspase-3 and -9 expressions. The effect was stronger than treatment cisplatin and echistatin alone. In this study, we have found that cisplatin plus echistatin treatment decreases collagen biosynthesis in MDA-MB-231 breast cancer cells stronger than the individual compounds. The inhibition was found to be dependent on the β1-integrin and IGF receptor activation. A significant reduction of ERK1/ERK2, AKT expression in cancer cells after cisplatin plus echistatin treatment was also found. The cancer cells treated by echistatin, cisplatin, and in particular the combination of both compounds drastically increased expression of NFκB transcription factor. Our results suggest that combined therapy cisplatin plus echistatin is a possible way to improve selectiveness of cisplatin. This

  14. Characterization of the glycosylation profile of the human breast cancer cell line, MDA-231, and a bone colonizing variant.

    PubMed

    Carcel-Trullols, Jaime; Stanley, Joseph S; Saha, Rinku; Shaaf, Saeid; Bendre, Manali S; Monzavi-Karbassi, Behjatolah; Suva, Larry J; Kieber-Emmons, Thomas

    2006-05-01

    The mechanisms that guide organ-specific metastases are not fully established. The aberrant expression of carbohydrates may play a fundamental role in defining the molecular mechanisms for metastases to distant organs and facilitate positive interactions within the target organ. The purpose of the present study was to determine the glycomic profile of a variant of the MDA-MB-231 breast cancer cell line that colonizes the bone and to ascribe mechanistic functions mediated by carbohydrates that might correlate with clinical bone metastases. The carbohydrate expression profiles of osteolytic MDA-MET breast cancer cells and non-osteolytic parental MDA-MB-231 cells were determined. MDA-MET cells were derived from MDA-MB-231 cells by in vivo selection of metastatic bone lesions following intracardiac inoculation. The two related breast cancer cell lines expressed distinct carbohydrate profiles; MDA-MET cells displayed an increased expression of alpha (2,6) linked sialic acid, N-beta1-6 GlcNAc, and sialylated Lewis-A antigen, and decreased expression of Galbeta1,3GalNAc as detected using a combination of lectins and anti-carbohydrate antibodies. Microarray analysis demonstrated an increased expression of glycosyltransferase genes, correlative for the distinct glycomic phenotype. The altered glycomic phenotypes of MDA-MET cells include effects on the differential binding to bone marrow endothelial cells, enhanced ECM binding and an increase in invasive potential. These data suggest that the glycomic phenotype of MDA-MET cells is associated with a select set of accumulated functions that collectively impact on the bone metastases and bone colonization capacity of breast cancer cells.

  15. Free Flight Rotorcraft Flight Test Vehicle Technology Development

    NASA Technical Reports Server (NTRS)

    Hodges, W. Todd; Walker, Gregory W.

    1994-01-01

    A rotary wing, unmanned air vehicle (UAV) is being developed as a research tool at the NASA Langley Research Center by the U.S. Army and NASA. This development program is intended to provide the rotorcraft research community an intermediate step between rotorcraft wind tunnel testing and full scale manned flight testing. The technologies under development for this vehicle are: adaptive electronic flight control systems incorporating artificial intelligence (AI) techniques, small-light weight sophisticated sensors, advanced telepresence-telerobotics systems and rotary wing UAV operational procedures. This paper briefly describes the system's requirements and the techniques used to integrate the various technologies to meet these requirements. The paper also discusses the status of the development effort. In addition to the original aeromechanics research mission, the technology development effort has generated a great deal of interest in the UAV community for related spin-off applications, as briefly described at the end of the paper. In some cases the technologies under development in the free flight program are critical to the ability to perform some applications.

  16. Innate Immune Sensing of Modified Vaccinia Virus Ankara (MVA) Is Mediated by TLR2-TLR6, MDA-5 and the NALP3 Inflammasome

    PubMed Central

    Delaloye, Julie; Roger, Thierry; Steiner-Tardivel, Quynh-Giao; Le Roy, Didier; Knaup Reymond, Marlies; Akira, Shizuo; Petrilli, Virginie; Gomez, Carmen E.; Perdiguero, Beatriz; Tschopp, Jürg; Pantaleo, Giuseppe; Esteban, Mariano; Calandra, Thierry

    2009-01-01

    Modified vaccinia virus Ankara (MVA) is an attenuated double-stranded DNA poxvirus currently developed as a vaccine vector against HIV/AIDS. Profiling of the innate immune responses induced by MVA is essential for the design of vaccine vectors and for anticipating potential adverse interactions between naturally acquired and vaccine-induced immune responses. Here we report on innate immune sensing of MVA and cytokine responses in human THP-1 cells, primary human macrophages and mouse bone marrow-derived macrophages (BMDMs). The innate immune responses elicited by MVA in human macrophages were characterized by a robust chemokine production and a fairly weak pro-inflammatory cytokine response. Analyses of the cytokine production profile of macrophages isolated from knockout mice deficient in Toll-like receptors (TLRs) or in the adapter molecules MyD88 and TRIF revealed a critical role for TLR2, TLR6 and MyD88 in the production of IFNβ-independent chemokines. MVA induced a marked up-regulation of the expression of RIG-I like receptors (RLR) and the IPS-1 adapter (also known as Cardif, MAVS or VISA). Reduced expression of RIG-I, MDA-5 and IPS-1 by shRNAs indicated that sensing of MVA by RLR and production of IFNβ and IFNβ-dependent chemokines was controlled by the MDA-5 and IPS-1 pathway in the macrophage. Crosstalk between TLR2-MyD88 and the NALP3 inflammasome was essential for expression and processing of IL-1β. Transcription of the Il1b gene was markedly impaired in TLR2−/− and MyD88−/− BMDM, whereas mature and secreted IL-1β was massively reduced in NALP3−/− BMDMs or in human THP-1 macrophages with reduced expression of NALP3, ASC or caspase-1 by shRNAs. Innate immune sensing of MVA and production of chemokines, IFNβ and IL-1β by macrophages is mediated by the TLR2-TLR6-MyD88, MDA-5-IPS-1 and NALP3 inflammasome pathways. Delineation of the host response induced by MVA is critical for improving our understanding of poxvirus antiviral escape

  17. Advanced flight software reconfiguraton

    NASA Technical Reports Server (NTRS)

    Porcher, Bryan

    1991-01-01

    Information is given in viewgraph form on advanced flight software reconfiguration. Reconfiguration is defined as identifying mission and configuration specific requirements, controlling mission and configuration specific data, binding this information to the flight software code to perform specific missions, and the release and distribution of the flight software. The objectives are to develop, demonstrate, and validate advanced software reconfiguration tools and techniques; to demonstrate reconfiguration approaches on Space Station Freedom (SSF) onboard systems displays; and to interactively test onboard systems displays, flight software, and flight data.

  18. Ubiquitin-Induced Oligomerization of the RNA Sensors RIG-I and MDA5 Activates Antiviral Innate Immune Response

    PubMed Central

    Jiang, Xiaomo; Kinch, Lisa; Brautigam, Chad A.; Chen, Xiang; Du, Fenghe; Grishin, Nick; Chen, Zhijian J.

    2012-01-01

    SUMMARY RIG-I and MDA5 detect viral RNA in the cytoplasm and activate signaling cascades leading to the production of type-I interferons. RIG-I is activated through sequential binding of viral RNA and unanchored lysine-63 (K63) polyubiquitin chains, but how polyubiquitin activates RIG-I and whether MDA5 is activated through a similar mechanism remain unresolved. Here we showed that the CARD domains of MDA5 bound to K63 polyubiquitin and that this binding was essential for MDA5 to activate the transcription factor IRF3. Mutations of conserved residues in MDA5 and RIG-I that disrupt their ubiquitin binding also abrogated their ability to activate IRF3. Polyubiquitin binding induced the formation of a large complex consisting of four RIG-I and four ubiquitin chains. This hetero-tetrameric complex was highly potent in activating the antiviral signaling cascades. These results suggest a unified mechanism of RIG-I and MDA5 activation and reveal a unique mechanism by which ubiquitin regulates cell signaling and immune response. PMID:22705106

  19. Flight Test Implementation of a Second Generation Intelligent Flight Control System

    NASA Technical Reports Server (NTRS)

    Williams-Hayes, Peggy S.

    2005-01-01

    The NASA F-15 Intelligent Flight Control System project team has developed a series of flight control concepts designed to demonstrate the benefits of a neural network-based adaptive controller. The objective of the team was to develop and flight-test control systems that use neural network technology, to optimize the performance of the aircraft under nominal conditions, and to stabilize the aircraft under failure conditions. Failure conditions include locked or failed control surfaces as well as unforeseen damage that might occur to the aircraft in flight. The Intelligent Flight Control System team is currently in the process of implementing a second generation control scheme, collectively known as Generation 2 or Gen 2, for flight testing on the NASA F-15 aircraft. This report describes the Gen 2 system as implemented by the team for flight test evaluation. Simulation results are shown which describe the experiment to be performed in flight and highlight the ways in which the Gen 2 system meets the defined objectives.

  20. Enantioselective degradation of amphetamine-like environmental micropollutants (amphetamine, methamphetamine, MDMA and MDA) in urban water.

    PubMed

    Evans, Sian E; Bagnall, John; Kasprzyk-Hordern, Barbara

    2016-08-01

    This paper aims to understand enantioselective transformation of amphetamine, methamphetamine, MDMA (3,4-methylenedioxy-methamphetamine) and MDA (3,4-methylenedioxyamphetamine) during wastewater treatment and in receiving waters. In order to undertake a comprehensive evaluation of the processes occurring, stereoselective transformation of amphetamine-like compounds was studied, for the first time, in controlled laboratory experiments: receiving water and activated sludge simulating microcosm systems. The results demonstrated that stereoselective degradation, via microbial metabolic processes favouring S-(+)-enantiomer, occurred in all studied amphetamine-based compounds in activated sludge simulating microcosms. R-(-)-enantiomers were not degraded (or their degradation was limited) which proves their more recalcitrant nature. Out of all four amphetamine-like compounds studied, amphetamine was the most susceptible to biodegradation. It was followed by MDMA and methamphetamine. Photochemical processes facilitated degradation of MDMA and methamphetamine but they were not, as expected, stereoselective. Preferential biodegradation of S-(+)-methamphetamine led to the formation of S-(+)-amphetamine. Racemic MDMA was stereoselectively biodegraded by activated sludge which led to its enrichment with R-(-)-enantiomer and formation of S-(+)-MDA. Interestingly, there was only mild stereoselectivity observed during MDMA degradation in rivers. This might be due to different microbial communities utilised during activated sludge treatment and those present in the environment. Kinetic studies confirmed the recalcitrant nature of MDMA.

  1. Chemical hazard evaluation of material disposal area (MDA) B closure project

    SciTech Connect

    Laul, Jagdish C

    2010-04-19

    TA-21, MDA-B (NES) is the 'contaminated dump,' landfill with radionuclides and chemicals from process waste disposed in 1940s. This paper focuses on chemical hazard categorization and hazard evaluation of chemicals of concern (e.g., peroxide, beryllium). About 170 chemicals were disposed in the landfill. Chemicals included products, unused and residual chemicals, spent, waste chemicals, non-flammable oils, mineral oil, etc. MDA-B was considered a High hazard site. However, based on historical records and best engineering judgment, the chemical contents are probably at best 5% of the chemical inventory. Many chemicals probably have oxidized, degraded or evaporated for volatile elements due to some fire and limited shelf-life over 60 yrs, which made it possible to downgrade from High to Low chemical hazard site. Knowing the site history and physical and chemical properties are very important in characterizing a NES site. Public site boundary is only 20 m, which is a major concern. Chemicals of concern during remediation are peroxide that can cause potential explosion and beryllium exposure due to chronic beryllium disease (CBD). These can be prevented or mitigated using engineering control (EC) and safety management program (SMP) to protect the involved workers and public.

  2. Anti-metastatic effect of Smilax china L. extract on MDA-MB-231 cells.

    PubMed

    Nho, Kyoung Jin; Chun, Jin Mi; Kim, Ho Kyoung

    2015-01-01

    Cancer metastases are not always cured by chemotherapy. Conventional and alternative drugs, including Chinese herbal remedies, have been developed to target metastatic cancer cells. Smilax china L. (SCL), a member of the Smilacaceae family, exerts anti-inflammatory, detoxification and anti-cancer effects. However, the effect of SCL on breast cancer cell metastasis and the underlying mechanisms are yet to be elucidated. The aim of this study was to investigate the effect of a SCL ethanol extract (SCLE) on the proliferation and migration of MDA-MB-231 human breast cancer cells, as well as the expression of urokinase plasminogen activator (uPA), uPA receptor (uPAR) and tissue inhibitors of metalloproteinases (TIMPs). Cell proliferation was assessed using the Cell Counting Kit‑8 and cell migration was determined by wound healing assay. Quantitative polymerase chain reaction was performed to quantify the mRNA levels of uPA, uPAR and TIMPs. SCLE markedly inhibited the proliferation and migration of MDA-MB-231 cells, and reduced the mRNA levels of the extracellular matrix (ECM) degradation-associated molecules uPA, uPAR. By contrast, SCLE significantly increased the mRNA levels of TIMP1 and TIMP2. These findings show that SCLE exerts an anti-metastatic effect on human breast cancer cells, which may involve the modulation of ECM degradation.

  3. 14 CFR 121.493 - Flight time limitations: Flight engineers and flight navigators.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Flight time limitations: Flight engineers and flight navigators. 121.493 Section 121.493 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... Limitations: Flag Operations § 121.493 Flight time limitations: Flight engineers and flight navigators. (a)...

  4. 14 CFR 121.493 - Flight time limitations: Flight engineers and flight navigators.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Flight time limitations: Flight engineers and flight navigators. 121.493 Section 121.493 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... Limitations: Flag Operations § 121.493 Flight time limitations: Flight engineers and flight navigators. (a)...

  5. 14 CFR 121.493 - Flight time limitations: Flight engineers and flight navigators.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Flight time limitations: Flight engineers and flight navigators. 121.493 Section 121.493 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... Limitations: Flag Operations § 121.493 Flight time limitations: Flight engineers and flight navigators. (a)...

  6. 14 CFR 121.493 - Flight time limitations: Flight engineers and flight navigators.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Flight time limitations: Flight engineers and flight navigators. 121.493 Section 121.493 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... Limitations: Flag Operations § 121.493 Flight time limitations: Flight engineers and flight navigators. (a)...

  7. 14 CFR 121.493 - Flight time limitations: Flight engineers and flight navigators.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Flight time limitations: Flight engineers and flight navigators. 121.493 Section 121.493 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... Limitations: Flag Operations § 121.493 Flight time limitations: Flight engineers and flight navigators. (a)...

  8. Novel function of MDA-9/Syntenin (SDCBP) as a regulator of survival and stemness in glioma stem cells

    PubMed Central

    Talukdar, Sarmistha; Das, Swadesh K.; Pradhan, Anjan K.; Emdad, Luni; Shen, Xue-Ning; Windle, Jolene J.; Sarkar, Devanand; Fisher, Paul B.

    2016-01-01

    Glioblastoma multiforme (GBM) is an aggressive cancer with current therapies only marginally impacting on patient survival. Glioma stem cells (GSCs), a subpopulation of highly tumorigenic cells, are considered major contributors to glioma progression and play seminal roles in therapy resistance, immune evasion and increased invasion. Despite clinical relevance, effective/selective therapeutic targeting strategies for GSCs do not exist, potentially due to the lack of a definitive understanding of key regulators of GSCs. Consequently, there is a pressing need to identify therapeutic targets and novel options to effectively target this therapy-resistant cell population. The precise roles of GSCs in governing GBM development, progression and prognosis are under intense scrutiny, but key upstream regulatory genes remain speculative. MDA-9/Syntenin (SDCBP), a scaffold protein, regulates tumor pathogenesis in multiple cancers. Highly aggressive cancers like GBM express elevated levels of MDA-9 and contain increased populations of GSCs. We now uncover a unique function of MDA-9 as a facilitator and determinant of glioma stemness and survival. Mechanistically, MDA-9 regulates multiple stemness genes (Nanog, Oct4 and Sox2) through activation of STAT3. MDA-9 controls survival of GSCs by activating the NOTCH1 pathway through phospho-Src and DLL1. Once activated, cleaved NOTCH1 regulates C-Myc expression through RBPJK, thereby facilitating GSC growth and proliferation. Knockdown of MDA-9 affects the NOTCH1/C-Myc and p-STAT3/Nanog pathways causing a loss of stemness and initiation of apoptosis in GSCs. Our data uncover a previously unidentified relationship between MDA-9 and GSCs, reinforcing relevance of this gene as a potential therapeutic target in GBM. PMID:27472461

  9. Integrated Neural Flight and Propulsion Control System

    NASA Technical Reports Server (NTRS)

    Kaneshige, John; Gundy-Burlet, Karen; Norvig, Peter (Technical Monitor)

    2001-01-01

    This paper describes an integrated neural flight and propulsion control system. which uses a neural network based approach for applying alternate sources of control power in the presence of damage or failures. Under normal operating conditions, the system utilizes conventional flight control surfaces. Neural networks are used to provide consistent handling qualities across flight conditions and for different aircraft configurations. Under damage or failure conditions, the system may utilize unconventional flight control surface allocations, along with integrated propulsion control, when additional control power is necessary for achieving desired flight control performance. In this case, neural networks are used to adapt to changes in aircraft dynamics and control allocation schemes. Of significant importance here is the fact that this system can operate without emergency or backup flight control mode operations. An additional advantage is that this system can utilize, but does not require, fault detection and isolation information or explicit parameter identification. Piloted simulation studies were performed on a commercial transport aircraft simulator. Subjects included both NASA test pilots and commercial airline crews. Results demonstrate the potential for improving handing qualities and significantly increasing survivability rates under various simulated failure conditions.

  10. Adaptive Controller Adaptation Time and Available Control Authority Effects on Piloting

    NASA Technical Reports Server (NTRS)

    Trujillo, Anna; Gregory, Irene

    2013-01-01

    Adaptive control is considered for highly uncertain, and potentially unpredictable, flight dynamics characteristic of adverse conditions. This experiment looked at how adaptive controller adaptation time to recover nominal aircraft dynamics affects pilots and how pilots want information about available control authority transmitted. Results indicate that an adaptive controller that takes three seconds to adapt helped pilots when looking at lateral and longitudinal errors. The controllability ratings improved with the adaptive controller, again the most for the three seconds adaptation time while workload decreased with the adaptive controller. The effects of the displays showing the percentage amount of available safe flight envelope used in the maneuver were dominated by the adaptation time. With the displays, the altitude error increased, controllability slightly decreased, and mental demand increased. Therefore, the displays did require some of the subjects resources but these negatives may be outweighed by pilots having more situation awareness of their aircraft.

  11. Flight code validation simulator

    SciTech Connect

    Sims, B.A.

    1995-08-01

    An End-To-End Simulation capability for software development and validation of missile flight software on the actual embedded computer has been developed utilizing a 486 PC, i860 DSP coprocessor, embedded flight computer and custom dual port memory interface hardware. This system allows real-time interrupt driven embedded flight software development and checkout. The flight software runs in a Sandia Digital Airborne Computer (SANDAC) and reads and writes actual hardware sensor locations in which IMU (Inertial Measurements Unit) data resides. The simulator provides six degree of freedom real-time dynamic simulation, accurate real-time discrete sensor data and acts on commands and discretes from the flight computer. This system was utilized in the development and validation of the successful premier flight of the Digital Miniature Attitude Reference System (DMARS) in January 1995 at the White Sands Missile Range on a two stage attitude controlled sounding rocket.

  12. Flight telerobotic servicer

    NASA Technical Reports Server (NTRS)

    Haley, Dennis

    1990-01-01

    Viewgraphs on the Space Station Flight Telerobotic Servicer (SSFTS) are presented. Topics covered include: SSFTS design; SSFTS elements; FTS mission requirements; FTS general requirements; flight telerobotic servicer - telerobot; FTS manipulator; force-torque transducer; end effector changeout mechanism; flight telerobotic servicer - end-of-arm tooling; user interfaces; FTS data management and processing; control subsystem; FTS vision subsystem and camera positioning assembly; FTS workstation display assembly panel; mini-master hand controller; and FTS NASREM system architecture.

  13. Digital flight control research

    NASA Technical Reports Server (NTRS)

    Potter, J. E.; Stern, R. G.; Smith, T. B.; Sinha, P.

    1974-01-01

    The results of studies which were undertaken to contribute to the design of digital flight control systems, particularly for transport aircraft are presented. In addition to the overall design considerations for a digital flight control system, the following topics are discussed in detail: (1) aircraft attitude reference system design, (2) the digital computer configuration, (3) the design of a typical digital autopilot for transport aircraft, and (4) a hybrid flight simulator.

  14. Autonomous Flight Safety System

    NASA Technical Reports Server (NTRS)

    Simpson, James

    2010-01-01

    The Autonomous Flight Safety System (AFSS) is an independent self-contained subsystem mounted onboard a launch vehicle. AFSS has been developed by and is owned by the US Government. Autonomously makes flight termination/destruct decisions using configurable software-based rules implemented on redundant flight processors using data from redundant GPS/IMU navigation sensors. AFSS implements rules determined by the appropriate Range Safety officials.

  15. Unified powered flight guidance

    NASA Technical Reports Server (NTRS)

    Brand, T. J.; Brown, D. W.; Higgins, J. P.

    1973-01-01

    A complete revision of the orbiter powered flight guidance scheme is presented. A unified approach to powered flight guidance was taken to accommodate all phases of exo-atmospheric orbiter powered flight, from ascent through deorbit. The guidance scheme was changed from the previous modified version of the Lambert Aim Point Maneuver Mode used in Apollo to one that employs linear tangent guidance concepts. This document replaces the previous ascent phase equation document.

  16. Development of flying qualities criteria for single pilot instrument flight operations

    NASA Technical Reports Server (NTRS)

    Bar-Gill, A.; Nixon, W. B.; Miller, G. E.

    1982-01-01

    Flying qualities criteria for Single Pilot Instrument Flight Rule (SPIFR) operations were investigated. The ARA aircraft was modified and adapted for SPIFR operations. Aircraft configurations to be flight-tested were chosen and matched on the ARA in-flight simulator, implementing modern control theory algorithms. Mission planning and experimental matrix design were completed. Microprocessor software for the onboard data acquisition system was debugged and flight-tested. Flight-path reconstruction procedure and the associated FORTRAN program were developed. Algorithms associated with the statistical analysis of flight test results and the SPIFR flying qualities criteria deduction are discussed.

  17. Bat flight: aerodynamics, kinematics and flight morphology.

    PubMed

    Hedenström, Anders; Johansson, L Christoffer

    2015-03-01

    Bats evolved the ability of powered flight more than 50 million years ago. The modern bat is an efficient flyer and recent research on bat flight has revealed many intriguing facts. By using particle image velocimetry to visualize wake vortices, both the magnitude and time-history of aerodynamic forces can be estimated. At most speeds the downstroke generates both lift and thrust, whereas the function of the upstroke changes with forward flight speed. At hovering and slow speed bats use a leading edge vortex to enhance the lift beyond that allowed by steady aerodynamics and an inverted wing during the upstroke to further aid weight support. The bat wing and its skeleton exhibit many features and control mechanisms that are presumed to improve flight performance. Whereas bats appear aerodynamically less efficient than birds when it comes to cruising flight, they have the edge over birds when it comes to manoeuvring. There is a direct relationship between kinematics and the aerodynamic performance, but there is still a lack of knowledge about how (and if) the bat controls the movements and shape (planform and camber) of the wing. Considering the relatively few bat species whose aerodynamic tracks have been characterized, there is scope for new discoveries and a need to study species representing more extreme positions in the bat morphospace.

  18. Flight research and testing

    NASA Technical Reports Server (NTRS)

    Putnam, Terrill W.; Ayers, Theodore G.

    1988-01-01

    Flight research and testing form a critical link in the aeronautic R and D chain. Brilliant concepts, elegant theories, and even sophisticated ground tests of flight vehicles are not sufficient to prove beyond doubt that an unproven aeronautical concept will actually perform as predicted. Flight research and testing provide the ultimate proof that an idea or concept performs as expected. Ever since the Wright brothers, flight research and testing have been the crucible in which aeronautical concepts have advanced and been proven to the point that engineers and companies have been willing to stake their future to produce and design new aircraft. This is still true today, as shown by the development of the experimental X-30 aerospace plane. The Dryden Flight Research Center (Ames-Dryden) continues to be involved in a number of flight research programs that require understanding and characterization of the total airplane in all the aeronautical disciplines, for example the X-29. Other programs such as the F-14 variable-sweep transition flight experiment have focused on a single concept or discipline. Ames-Dryden also continues to conduct flight and ground based experiments to improve and expand the ability to test and evaluate advanced aeronautical concepts. A review of significant aeronautical flight research programs and experiments is presented to illustrate both the progress made and the challenges to come.

  19. Flight research and testing

    NASA Technical Reports Server (NTRS)

    Putnam, Terrill W.; Ayers, Theodore G.

    1989-01-01

    Flight research and testing form a critical link in the aeronautic research and development chain. Brilliant concepts, elegant theories, and even sophisticated ground tests of flight vehicles are not sufficient to prove beyond a doubt that an unproven aeronautical concept will actually perform as predicted. Flight research and testing provide the ultimate proof that an idea or concept performs as expected. Ever since the Wright brothers, flight research and testing were the crucible in which aeronautical concepts were advanced and proven to the point that engineers and companies are willing to stake their future to produce and design aircraft. This is still true today, as shown by the development of the experimental X-30 aerospace plane. The Dryden Flight Research Center (Ames-Dryden) continues to be involved in a number of flight research programs that require understanding and characterization of the total airplane in all the aeronautical disciplines, for example the X-29. Other programs such as the F-14 variable-sweep transition flight experiment have focused on a single concept or discipline. Ames-Dryden also continues to conduct flight and ground based experiments to improve and expand the ability to test and evaluate advanced aeronautical concepts. A review of significant aeronautical flight research programs and experiments is presented to illustrate both the progress being made and the challenges to come.

  20. The NASA F-15 Intelligent Flight Control Systems: Generation II

    NASA Technical Reports Server (NTRS)

    Buschbacher, Mark; Bosworth, John

    2006-01-01

    The Second Generation (Gen II) control system for the F-15 Intelligent Flight Control System (IFCS) program implements direct adaptive neural networks to demonstrate robust tolerance to faults and failures. The direct adaptive tracking controller integrates learning neural networks (NNs) with a dynamic inversion control law. The term direct adaptive is used because the error between the reference model and the aircraft response is being compensated or directly adapted to minimize error without regard to knowing the cause of the error. No parameter estimation is needed for this direct adaptive control system. In the Gen II design, the feedback errors are regulated with a proportional-plus-integral (PI) compensator. This basic compensator is augmented with an online NN that changes the system gains via an error-based adaptation law to improve aircraft performance at all times, including normal flight, system failures, mispredicted behavior, or changes in behavior resulting from damage.

  1. Design of a Computerised Flight Mill Device to Measure the Flight Potential of Different Insects

    PubMed Central

    Martí-Campoy, Antonio; Ávalos, Juan Antonio; Soto, Antonia; Rodríguez-Ballester, Francisco; Martínez-Blay, Victoria; Malumbres, Manuel Pérez

    2016-01-01

    Several insect species pose a serious threat to different plant species, sometimes becoming a pest that produces significant damage to the landscape, biodiversity, and/or the economy. This is the case of Rhynchophorus ferrugineus Olivier (Coleoptera: Dryophthoridae), Semanotus laurasii Lucas (Coleoptera: Cerambycidae), and Monochamus galloprovincialis Olivier (Coleoptera: Cerambycidae), which have become serious threats to ornamental and productive trees all over the world such as palm trees, cypresses, and pines. Knowledge about their flight potential is very important for designing and applying measures targeted to reduce the negative effects from these pests. Studying the flight capability and behaviour of some insects is difficult due to their small size and the large area wherein they can fly, so we wondered how we could obtain information about their flight capabilities in a controlled environment. The answer came with the design of flight mills. Relevant data about the flight potential of these insects may be recorded and analysed by means of a flight mill. Once an insect is attached to the flight mill, it is able to fly in a circular direction without hitting walls or objects. By adding sensors to the flight mill, it is possible to record the number of revolutions and flight time. This paper presents a full description of a computer monitored flight mill. The description covers both the mechanical and the electronic parts in detail. The mill was designed to easily adapt to the anatomy of different insects and was successfully tested with individuals from three species R. ferrugineus, S. laurasii, and M. galloprovincialis. PMID:27070600

  2. Cell cycle arrest induced by MPPa-PDT in MDA-MB-231 cells

    NASA Astrophysics Data System (ADS)

    Liang, Liming; Bi, Wenxiang; Tian, Yuanyuan

    2016-05-01

    Photodynamic therapy (PDT) is a medical treatment using a photosensitizing agent and light source to treat cancers. Pyropheophorbidea methyl ester (MPPa), a derivative of chlorophyll, is a novel potent photosensitizer. To learn more about this photosensitizer, we examined the cell cycle arrest in MDA-MB-231. Cell cycle and apoptosis were measured by flow cytometer. Checkpoints of the cell cycle were measured by western blot. In this study, we found that the expression of Cyclin D1 was obviously decreased, while the expression of Chk2 and P21 was increased after PDT treatment. This study showed that MPPa-PDT affected the checkpoints of the cell cycle and led the cells to apoptosis.

  3. Investigating the Mechanisms of Hallucinogen-Induced Visions Using 3,4-Methylenedioxyamphetamine (MDA): A Randomized Controlled Trial in Humans

    PubMed Central

    Baggott, Matthew J.; Siegrist, Jennifer D.; Galloway, Gantt P.; Robertson, Lynn C.; Coyle, Jeremy R.; Mendelson, John E.

    2010-01-01

    Background The mechanisms of drug-induced visions are poorly understood. Very few serotonergic hallucinogens have been studied in humans in decades, despite widespread use of these drugs and potential relevance of their mechanisms to hallucinations occurring in psychiatric and neurological disorders. Methodology/Principal Findings We investigated the mechanisms of hallucinogen-induced visions by measuring the visual and perceptual effects of the hallucinogenic serotonin 5-HT2AR receptor agonist and monoamine releaser, 3,4-methylenedioxyamphetamine (MDA), in a double-blind placebo-controlled study. We found that MDA increased self-report measures of mystical-type experience and other hallucinogen-like effects, including reported visual alterations. MDA produced a significant increase in closed-eye visions (CEVs), with considerable individual variation. Magnitude of CEVs after MDA was associated with lower performance on measures of contour integration and object recognition. Conclusions/Significance Drug-induced visions may have greater intensity in people with poor sensory or perceptual processing, suggesting common mechanisms with other hallucinatory syndromes. MDA is a potential tool to investigate mystical experiences and visual perception. Trial Registration Clinicaltrials.gov NCT00823407 PMID:21152030

  4. Anti-Cancer Effect of IN-2001 in MDA-MB-231 Human Breast Cancer.

    PubMed

    Min, Kyung Nan; Joung, Ki Eun; Kim, Dae-Kee; Sheen, Yhun Yhong

    2012-05-01

    In recent years, inhibition of HDACs has emerged as a potential strategy to reverse aberrant epigenetic changes associated with cancer, and several classes of HDAC inhibitors have been found to have potent and specific anticancer activities in preclinical studies. But their precise mechanism of action has not been elucidated. In this study, a novel synthetic inhibitor of HDAC, 3-(4-dimethylamino phenyl)-N-hydroxy-2-propenamide [IN-2001] was examined for its antitumor activity and the underlying molecular mechanisms of any such activity on human breast cancer cell lines. IN-2001 effectively inhibited cellular HDAC activity (IC50 = 0.585 nM) in MDA-MB-231 human breast cancer cells. IN-2001 caused a significant dose-dependent inhibition of cell proliferation in estrogen receptor (ER) negative MDA-MB-231 human breast cancer cells. Cell cycle analysis revealed that the gowth inhibitory effects of IN-2001 might be attributed to cell cycle arrest at G0/G1 and/or G2/Mphase and subsequent apoptosis in human breast cancer cells. These events are accompanied by modulating several cell cycle and apoptosis regulatory genes such as CDK inhibitors p21(WAF1) and p27(KIP1) cyclin D1, and other tumor suppressor genes such as cyclin D2. Collectively, IN-2001 inhibited cell proliferation and induced apoptosis in human breast cancer cells and these findings may provide new therapeutic approaches, combination of antiestrogen together with a HDAC inhibitor, in the hormonal therapy-resistant ER-negative breast cancers. In summary, our data suggest that this histone deacetylase inhibitor, IN-2001, is a novel promising therapeutic agent with potent antitumor effects against human breast cancers.

  5. Recent success on SLS FPAs and MDA's new direction for development

    NASA Astrophysics Data System (ADS)

    Tidrow, Meimei Z.; Zheng, Lucy; Barcikowski, Hank; Wells, James; Aitcheson, Leslie

    2009-05-01

    Over the past few years, the Missile Defense Agency Advanced Technology Directorate (MDA/DV) has funded the development of a new III-V infrared (IR) sensor focal plane material: type II strained layer superlattice (SLS). Infrared sensors are crucial to missile defense capabilities for target acquisition, tracking, discrimination, and aim point selection; they serve other military sensing applications as well. Most current infrared military systems use mercury-cadmiumtelluride (HgCdTe), a II-VI semiconductor material, for long-wavelength (LW) (8-12 um) focal plane array (FPA) applications. It is difficult to achieve large-format FPAs in HgCdTe at long wavelengths (LW) due to their low yield. The situation is aggravated by the limitation of the small cadmium-zinc-telluride (CdZnTe) substrates. SLS is the only known IR material that has a theoretical prediction of higher performance than HgCdTe. Over the past three years, SLS technology has progressed significantly, demonstrating experimentally its potential as a strong candidate for future highperformance IR sensor materials. In this paper, we will discuss the most recent progress made in SLS. We will also discuss MDA's new direction for this technology development. The plan is to use a horizontal integration approach instead of adhering to the existing vertical integration model. This new horizontal approach is to increase the number of industrial participants working in SLS and leverage existing III-V semiconductor foundries. Hopefully it will reduce the cost of SLS IR technology development, shared foundry maintenance, and future SLS production.

  6. Technology review of flight crucial flight controls

    NASA Technical Reports Server (NTRS)

    Rediess, H. A.; Buckley, E. C.

    1984-01-01

    The results of a technology survey in flight crucial flight controls conducted as a data base for planning future research and technology programs are provided. Free world countries were surveyed with primary emphasis on the United States and Western Europe because that is where the most advanced technology resides. The survey includes major contemporary systems on operational aircraft, R&D flight programs, advanced aircraft developments, and major research and technology programs. The survey was not intended to be an in-depth treatment of the technology elements, but rather a study of major trends in systems level technology. The information was collected from open literature, personal communications and a tour of several companies, government organizations and research laboratories in the United States, United Kingdom, France, and the Federal Republic of Germany.

  7. Operator versus computer control of adaptive automation

    NASA Technical Reports Server (NTRS)

    Hilburn, Brian; Molloy, Robert; Wong, Dick; Parasuraman, Raja

    1993-01-01

    Adaptive automation refers to real-time allocation of functions between the human operator and automated subsystems. The article reports the results of a series of experiments whose aim is to examine the effects of adaptive automation on operator performance during multi-task flight simulation, and to provide an empirical basis for evaluations of different forms of adaptive logic. The combined results of these studies suggest several things. First, it appears that either excessively long, or excessively short, adaptation cycles can limit the effectiveness of adaptive automation in enhancing operator performance of both primary flight and monitoring tasks. Second, occasional brief reversions to manual control can counter some of the monitoring inefficiency typically associated with long cycle automation, and further, that benefits of such reversions can be sustained for some time after return to automated control. Third, no evidence was found that the benefits of such reversions depend on the adaptive logic by which long-cycle adaptive switches are triggered.

  8. Somatosensory Substrates of Flight Control in Bats

    PubMed Central

    Marshall, Kara L.; Chadha, Mohit; deSouza, Laura A.; Sterbing-D’Angelo, Susanne J.; Moss, Cynthia F.; Lumpkin, Ellen A.

    2015-01-01

    Summary Flight maneuvers require rapid sensory integration to generate adaptive motor output. Bats achieve remarkable agility with modified forelimbs that serve as airfoils while retaining capacity for object manipulation. Wing sensory inputs provide behaviorally relevant information to guide flight; however, components of wing sensory-motor circuits have not been analyzed. Here, we elucidate the organization of wing innervation in an insectivore, the big brown bat, Eptesicus fuscus. We demonstrate that wing sensory innervation differs from other vertebrate forelimbs, revealing a peripheral basis for the atypical topographic organization reported for bat somatosensory nuclei. Furthermore, the wing is innervated by an unusual complement of sensory neurons poised to report airflow and touch. Finally, we report that cortical neurons encode tactile and airflow inputs with sparse activity patterns. Together, our findings identify neural substrates of somatosensation in the bat wing and imply that evolutionary pressures giving rise to mammalian flight led to unusual sensorimotor projections. PMID:25937277

  9. X-43A Flight Controls

    NASA Technical Reports Server (NTRS)

    Baumann, Ethan

    2006-01-01

    A viewgraph presentation detailing X-43A Flight controls at NASA Dryden Flight Research Center is shown. The topics include: 1) NASA Dryden, Overview and current and recent flight test programs; 2) Unmanned Aerial Vehicle Synthetic Aperture Radar (UAVSAR) Program, Program Overview and Platform Precision Autopilot; and 3) Hyper-X Program, Program Overview, X-43A Flight Controls and Flight Results.

  10. Screening for basic drugs in hair of drug addicts by liquid chromatography/time-of-flight mass spectrometry.

    PubMed

    Pelander, Anna; Ristimaa, Johanna; Rasanen, Ilpo; Vuori, Erkki; Ojanperä, Ilkka

    2008-12-01

    Hair analysis in forensic and clinical toxicology has been strongly focused on drugs of abuse, and comprehensive, drug class-independent screening methods based on mass spectrometric detection have not been applied to date. In this study, a qualitative drug screening method by liquid chromatography coupled to time-of-flight mass spectrometry, earlier developed and evaluated for forensic toxicological urine analysis, was adapted for screening of basic drugs in hair. The method included alkaline hydrolysis, purification with mixed-mode solid phase extraction, and analysis by liquid chromatography coupled to time-of-flight mass spectrometry with automated data analysis and reporting. Identification was based on accurate mass, isotopic pattern fit, and retention time, if available. Analysis of 32 hair samples from deceased drug addicts revealed 35 different drugs. The drug classes identified included antidepressants, antipsychotics, antiepileptics, amphetamines, opioids, beta-blockers, a benzodiazepine, a hypnotic, a local anesthetic, an antiemetic, and an antipyretic analgesic. The findings were in good agreement with the findings in blood and urine by other methods. Moreover, information about previous drug use not evident in the analysis of other matrices was obtained in the majority (72%) of the cases. Tramadol was an especially predominant finding, suggesting tramadol abuse as an opioid substitute. One apparent false-positive finding was identified. The mean and median mass accuracies of positive findings were 2.3 and 1.8 ppm, corresponding to 0.5 and 0.4 mDa, respectively. Cutoff values for tramadol and methamphetamine in hair were 100 and 200 pg/mg, respectively. The method proved to be a simple and straightforward tool for comprehensive screening of basic drugs in hair.

  11. Space Flight. Teacher Resources.

    ERIC Educational Resources Information Center

    2001

    This teacher's guide contains information, lesson plans, and diverse student learning activities focusing on space flight. The guide is divided into seven sections: (1) "Drawing Activities" (Future Flight; Space Fun; Mission: Draw); (2) "Geography" (Space Places); (3) "History" (Space and Time); (4)…

  12. Flight Test Techniques

    DTIC Science & Technology

    2009-07-01

    Fort Rucker, AL 36362-5276 8. PERFORMING ORGANIZATION REPORT NUMBER TOP 7-4-020 9. SPONSORING/ MONITORING AGENCY NAME(S) AND ADDRESS(ES...2 3. REQUIRED TEST CONDITIONS ............................................. 3 3.1...3. REQUIRED TEST CONDITIONS . 3.1 Air Vehicle Flight Test Techniques. Many different flight test techniques are in existence. As technology

  13. Electromechanical flight control actuator

    NASA Technical Reports Server (NTRS)

    1979-01-01

    The feasibility of using an electromechanical actuator (EMA) as the primary flight control equipment in aerospace flight is examined. The EMA motor design is presented utilizing improved permanent magnet materials. The necessary equipment to complete a single channel EMA using the single channel power electronics breadboard is reported. The design and development of an improved rotor position sensor/tachometer is investigated.

  14. Exploring flight crew behaviour

    NASA Technical Reports Server (NTRS)

    Helmreich, R. L.

    1987-01-01

    A programme of research into the determinants of flight crew performance in commercial and military aviation is described, along with limitations and advantages associated with the conduct of research in such settings. Preliminary results indicate significant relationships among personality factors, attitudes regarding flight operations, and crew performance. The potential theoretical and applied utility of the research and directions for further research are discussed.

  15. Autonomous Flight Safety System

    NASA Technical Reports Server (NTRS)

    Ferrell, Bob; Santuro, Steve; Simpson, James; Zoerner, Roger; Bull, Barton; Lanzi, Jim

    2004-01-01

    Autonomous Flight Safety System (AFSS) is an independent flight safety system designed for small to medium sized expendable launch vehicles launching from or needing range safety protection while overlying relatively remote locations. AFSS replaces the need for a man-in-the-loop to make decisions for flight termination. AFSS could also serve as the prototype for an autonomous manned flight crew escape advisory system. AFSS utilizes onboard sensors and processors to emulate the human decision-making process using rule-based software logic and can dramatically reduce safety response time during critical launch phases. The Range Safety flight path nominal trajectory, its deviation allowances, limit zones and other flight safety rules are stored in the onboard computers. Position, velocity and attitude data obtained from onboard global positioning system (GPS) and inertial navigation system (INS) sensors are compared with these rules to determine the appropriate action to ensure that people and property are not jeopardized. The final system will be fully redundant and independent with multiple processors, sensors, and dead man switches to prevent inadvertent flight termination. AFSS is currently in Phase III which includes updated algorithms, integrated GPS/INS sensors, large scale simulation testing and initial aircraft flight testing.

  16. Java for flight software

    NASA Technical Reports Server (NTRS)

    Benowitz, E.; Niessner, A.

    2003-01-01

    This work involves developing representative mission-critical spacecraft software using the Real-Time Specification for Java (RTSJ). This work currently leverages actual flight software used in the design of actual flight software in the NASA's Deep Space 1 (DSI), which flew in 1998.

  17. Overview With Results and Lessons Learned of the X-43A Mach 10 Flight

    NASA Technical Reports Server (NTRS)

    Marshall, Laurie A.; Bahm, Catherine; Corpening, Griffin P.; Sherrill, Robert

    2005-01-01

    This paper provides an overview of the final flight of the NASA X-43A project. The project consisted of three flights, two planned for Mach 7 and one for Mach 10. The third and final flight, November 16, 2004, was the first Mach 10 flight demonstration of an airframe-integrated, scramjet-powered, hypersonic vehicle. The goals and objectives for the project as well as those for the third flight are presented. The configuration of the Hyper-X stack including the X-43A, Hyper-X launch vehicle, and Hyper-X research vehicle adapter is discussed. The second flight of the X-43A was successfully conducted on March 27, 2004. Mission differences, vehicle modifications and lessons learned from the second flight as they applied to the third flight are also discussed. An overview of flight 3 results is presented.

  18. Disruption of postural readaptation by inertial stimuli following space flight

    NASA Technical Reports Server (NTRS)

    Black, F. O.; Paloski, W. H.; Reschke, M. F.; Igarashi, M.; Guedry, F.; Anderson, D. J.

    1999-01-01

    Postural instability (relative to pre-flight) has been observed in all shuttle astronauts studied upon return from orbital missions. Postural stability was more closely examined in four shuttle astronaut subjects before and after an 8 day orbital mission. Results of the pre- and post-flight postural stability studies were compared with a larger (n = 34) study of astronauts returning from shuttle missions of similar duration. Results from both studies indicated that inadequate vestibular feedback was the most significant sensory deficit contributing to the postural instability observed post flight. For two of the four IML-1 astronauts, post-flight postural instability and rate of recovery toward their earth-normal performance matched the performance of the larger sample. However, post-flight postural control in one returning astronaut was substantially below mean performance. This individual, who was within normal limits with respect to postural control before the mission, indicated that recovery to pre-flight postural stability was also interrupted by a post-flight pitch plane rotation test. A similar, though less extreme departure from the mean recovery trajectory was present in another astronaut following the same post-flight rotation test. The pitch plane rotation stimuli included otolith stimuli in the form of both transient tangential and constant centripetal linear acceleration components. We inferred from these findings that adaptation on orbit and re-adaptation on earth involved a change in sensorimotor integration of vestibular signals most likely from the otolith organs.

  19. Disruption of postural readaptation by inertial stimuli following space flight.

    PubMed

    Black, F O; Paloski, W H; Reschke, M F; Igarashi, M; Guedry, F; Anderson, D J

    1999-01-01

    Postural instability (relative to pre-flight) has been observed in all shuttle astronauts studied upon return from orbital missions. Postural stability was more closely examined in four shuttle astronaut subjects before and after an 8 day orbital mission. Results of the pre- and post-flight postural stability studies were compared with a larger (n = 34) study of astronauts returning from shuttle missions of similar duration. Results from both studies indicated that inadequate vestibular feedback was the most significant sensory deficit contributing to the postural instability observed post flight. For two of the four IML-1 astronauts, post-flight postural instability and rate of recovery toward their earth-normal performance matched the performance of the larger sample. However, post-flight postural control in one returning astronaut was substantially below mean performance. This individual, who was within normal limits with respect to postural control before the mission, indicated that recovery to pre-flight postural stability was also interrupted by a post-flight pitch plane rotation test. A similar, though less extreme departure from the mean recovery trajectory was present in another astronaut following the same post-flight rotation test. The pitch plane rotation stimuli included otolith stimuli in the form of both transient tangential and constant centripetal linear acceleration components. We inferred from these findings that adaptation on orbit and re-adaptation on earth involved a change in sensorimotor integration of vestibular signals most likely from the otolith organs.

  20. Effect of Polygonatum odoratum extract on human breast cancer MDA-MB-231 cell proliferation and apoptosis

    PubMed Central

    Tai, Yu; Sun, Yi-Ming; Zou, Xue; Pan, Qiong; Lan, Ya-Dong; Huo, Qiang; Zhu, Jing-Wen; Guo, Fei; Zheng, Chang-Quan; Wu, Cheng-Zhu; Liu, Hao

    2016-01-01

    Traditional Chinese medicine (TCM) is important in the provision of anti-tumor drugs. Recently, studies have shown that certain types of TCM agents are able to control the growth of tumors, enhance the body's immune function and enhance the therapeutic effect of chemotherapeutic drugs. In women, breast carcinoma is the most common tumor type and the second most common cause of death from cancer. Polygonatum odoratum (P. odoratum) is commonly used in TCM. The aim of the present study was to investigate the effects of P. odoratum extract on the proliferation and apoptosis of MDA-MB-231 breast cancer cells. Cell proliferation was assessed using MTT and colony formation assays. In addition, propidium iodide (PI)/Annexin V-FITC staining was used to investigate the apoptosis of MDA-MB-231 cells following treatment with P. odoratum extract. The protein expression levels of B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax) were also detected using western blot analysis, while a JC-1 staining assay was used to assess the mitochondrial membrane potential (ΔΨm). The results of the MTT assay showed that the proliferation and colony formation of MDA-MB-231 cells were inhibited following treatment with the extract. Furthermore, the PI/Annexin-V staining showed that the apoptosis of MDA-MB-231 cells was enhanced by the extract, in a concentration-dependent manner. The extract also lowered the ΔΨm of MDA-MB-231 cells, upregulated the expression of Bax and inhibited the expression of Bcl-2. In conclusion, these results showed that the P. odoratum extract inhibited the proliferation and induced apoptosis of breast cancer MDA-MB-231 cells. PMID:27698772

  1. SLS-1 flight experiments preliminary significant results

    NASA Astrophysics Data System (ADS)

    1992-01-01

    Spacelab Life Sciences-1 (SLS-1) is the first of a series of dedicated life sciences Spacelab missions designed to investigate the mechanisms involved in the physiological adaptation to weightlessness and the subsequent readaptation to 1 gravity (1 G). Hypotheses generated from the physiological effects observed during earlier missions led to the formulation of several integrated experiments to determine the underlying mechanisms responsible for the observed phenomena. The 18 experiments selected for flight on SLS-1 investigated the cardiovascular, cardiopulmonary, regulatory physiology, musculoskeletal, and neuroscience disciplines in both human and rodent subjects. The SLS-1 preliminary results gave insight to the mechanisms involved in the adaptation to the microgravity environment and readaptation when returning to Earth. The experimental results will be used to promote health and safety for future long duration space flights and, as in the past, will be applied to many biomedical problems encountered here on Earth.

  2. The flight of Archaeopteryx.

    PubMed

    Chatterjee, Sankar; Templin, R Jack

    2003-01-01

    The origin of avian flight is often equated with the phylogeny, ecology, and flying ability of the primitive Jurassic bird, Archaeopteryx. Debate persists about whether it was a terrestrial cursor or a tree dweller. Despite broad acceptance of its arboreal life style from anatomical, phylogenetic, and ecological evidence, a new version of the cursorial model was proposed recently asserting that a running Archaeopteryx could take off from the ground using thrust and sustain flight in the air. However, Archaeopteryx lacked both the powerful flight muscles and complex wing movements necessary for ground takeoff. Here we describe a flight simulation model, which suggests that for Archaeopteryx, takeoff from a perch would have been more efficient and cost-effective than from the ground. Archaeopteryx may have made short flights between trees, utilizing a novel method of phugoid gliding.

  3. Miscarriage Among Flight Attendants

    PubMed Central

    Grajewski, Barbara; Whelan, Elizabeth A.; Lawson, Christina C.; Hein, Misty J.; Waters, Martha A.; Anderson, Jeri L.; MacDonald, Leslie A.; Mertens, Christopher J.; Tseng, Chih-Yu; Cassinelli, Rick T.; Luo, Lian

    2015-01-01

    Background Cosmic radiation and circadian disruption are potential reproductive hazards for flight attendants. Methods Flight attendants from 3 US airlines in 3 cities were interviewed for pregnancy histories and lifestyle, medical, and occupational covariates. We assessed cosmic radiation and circadian disruption from company records of 2 million individual flights. Using Cox regression models, we compared respondents (1) by levels of flight exposures and (2) to teachers from the same cities, to evaluate whether these exposures were associated with miscarriage. Results Of 2654 women interviewed (2273 flight attendants and 381 teachers), 958 pregnancies among 764 women met study criteria. A hypothetical pregnant flight attendant with median firsttrimester exposures flew 130 hours in 53 flight segments, crossed 34 time zones, and flew 15 hours during her home-base sleep hours (10 pm–8 am), incurring 0.13 mGy absorbed dose (0.36 mSv effective dose) of cosmic radiation. About 2% of flight attendant pregnancies were likely exposed to a solar particle event, but doses varied widely. Analyses suggested that cosmic radiation exposure of 0.1 mGy or more may be associated with increased risk of miscarriage in weeks 9–13 (odds ratio = 1.7 [95% confidence interval = 0.95–3.2]). Risk of a first-trimester miscarriage with 15 hours or more of flying during home-base sleep hours was increased (1.5 [1.1–2.2]), as was risk with high physical job demands (2.5 [1.5–4.2]). Miscarriage risk was not increased among flight attendants compared with teachers. Conclusions Miscarriage was associated with flight attendant work during sleep hours and high physical job demands and may be associated with cosmic radiation exposure. PMID:25563432

  4. STS-111 Flight Day 3 Highlights

    NASA Technical Reports Server (NTRS)

    2002-01-01

    On Flight Day 3 of STS-111, the crew of Endeavour (Kenneth Cockrell, Commander; Paul Lockhart, Pilot; Franklin Chang-Diaz, Mission Specialist; Philippe Perrin, Mission Specialist) and the Expedition 5 crew (Valery Korzun, Commander; Peggy Whitson, Flight Engineer; Sergei Treschev, Flight Engineer), begin their final approach towards the International Space Station (ISS). From cameras aboard the ISS, live video of Endeavour is shown as it approaches the station. The Orbiter is maneuvered slowly to a position for docking, and cameras from multiple angles show this process. As it is maneuvered, there are clear views of its payload bay, which includes the Leonardo MultiPurpose Logistics Module (MPLM) and the Mobile Base System (MBS), both of which will be installed on the ISS during this mission. In the final stages of the docking procedure there is close-up footage of Endeavour meeting the ISS's Pressurized Mating Adapter 2 on the Destiny Laboratory Module. Inside the ISS, the Expedition 4 crew (Yury Onufrienko, Commander; Daniel Bursch, Flight Engineer; Carl Walz, Flight Engineer), which will be replaced by the Expedition 5 crew, prepares for final docking. Crew members are shown transferring equipment from the Endeavour to the ISS, prior to a replay of the mating of the two crafts. In the replay, the hatch is shown being opened and the two newly arrived crews are greeted with excitement by Expedition 4 crewmembers. The video closes with footage of the Quest airlock used for EVA (extravehicular activity) egress, and the Canadarm 2 robotic arm.

  5. Ethernet for Space Flight Applications

    NASA Technical Reports Server (NTRS)

    Webb, Evan; Day, John H. (Technical Monitor)

    2002-01-01

    NASA's Goddard Space Flight Center (GSFC) is adapting current data networking technologies to fly on future spaceflight missions. The benefits of using commercially based networking standards and protocols have been widely discussed and are expected to include reduction in overall mission cost, shortened integration and test (I&T) schedules, increased operations flexibility, and hardware and software upgradeability/scalability with developments ongoing in the commercial world. The networking effort is a comprehensive one encompassing missions ranging from small University Explorer (UNEX) class spacecraft to large observatories such as the Next Generation Space Telescope (NGST). Mission aspects such as flight hardware and software, ground station hardware and software, operations, RF communications, and security (physical and electronic) are all being addressed to ensure a complete end-to-end system solution. One of the current networking development efforts at GSFC is the SpaceLAN (Spacecraft Local Area Network) project, development of a space-qualifiable Ethernet network. To this end we have purchased an IEEE 802.3-compatible 10/100/1000 Media Access Control (MAC) layer Intellectual Property (IP) core and are designing a network node interface (NNI) and associated network components such as a switch. These systems will ultimately allow the replacement of the typical MIL-STD-1553/1773 and custom interfaces that inhabit most spacecraft. In this paper we will describe our current Ethernet NNI development along with a novel new space qualified physical layer that will be used in place of the standard interfaces. We will outline our plans for development of space qualified network components that will allow future spacecraft to operate in significant radiation environments while using a single onboard network for reliable commanding and data transfer. There will be a brief discussion of some issues surrounding system implications of a flight Ethernet. Finally, we will

  6. Future Flight Decks

    NASA Technical Reports Server (NTRS)

    Arbuckle, P. Douglas; Abbott, Kathy H.; Abbott, Terence S.; Schutte, Paul C.

    1998-01-01

    The evolution of commercial transport flight deck configurations over the past 20-30 years and expected future developments are described. Key factors in the aviation environment are identified that the authors expect will significantly affect flight deck designers. One of these is the requirement for commercial aviation accident rate reduction, which is probably required if global commercial aviation is to grow as projected. Other factors include the growing incrementalism in flight deck implementation, definition of future airspace operations, and expectations of a future pilot corps that will have grown up with computers. Future flight deck developments are extrapolated from observable factors in the aviation environment, recent research results in the area of pilot-centered flight deck systems, and by considering expected advances in technology that are being driven by other than aviation requirements. The authors hypothesize that revolutionary flight deck configuration changes will be possible with development of human-centered flight deck design methodologies that take full advantage of commercial and/or entertainment-driven technologies.

  7. Bat flight and zoonotic viruses

    USGS Publications Warehouse

    O'Shea, Thomas J.; Cryan, Paul M.; Cunningham, Andrew A.; Fooks, Anthony R.; Hayman, David T.S.; Luis, Angela D.; Peel, Alison J.; Plowright, Raina K.; Wood, James L.N.

    2014-01-01

    Bats are sources of high viral diversity and high-profile zoonotic viruses worldwide. Although apparently not pathogenic in their reservoir hosts, some viruses from bats severely affect other mammals, including humans. Examples include severe acute respiratory syndrome coronaviruses, Ebola and Marburg viruses, and Nipah and Hendra viruses. Factors underlying high viral diversity in bats are the subject of speculation. We hypothesize that flight, a factor common to all bats but to no other mammals, provides an intensive selective force for coexistence with viral parasites through a daily cycle that elevates metabolism and body temperature analogous to the febrile response in other mammals. On an evolutionary scale, this host–virus interaction might have resulted in the large diversity of zoonotic viruses in bats, possibly through bat viruses adapting to be more tolerant of the fever response and less virulent to their natural hosts.

  8. Bat Flight and Zoonotic Viruses

    PubMed Central

    Cryan, Paul M.; Cunningham, Andrew A.; Fooks, Anthony R.; Hayman, David T.S.; Luis, Angela D.; Peel, Alison J.; Plowright, Raina K.; Wood, James L.N.

    2014-01-01

    Bats are sources of high viral diversity and high-profile zoonotic viruses worldwide. Although apparently not pathogenic in their reservoir hosts, some viruses from bats severely affect other mammals, including humans. Examples include severe acute respiratory syndrome coronaviruses, Ebola and Marburg viruses, and Nipah and Hendra viruses. Factors underlying high viral diversity in bats are the subject of speculation. We hypothesize that flight, a factor common to all bats but to no other mammals, provides an intensive selective force for coexistence with viral parasites through a daily cycle that elevates metabolism and body temperature analogous to the febrile response in other mammals. On an evolutionary scale, this host–virus interaction might have resulted in the large diversity of zoonotic viruses in bats, possibly through bat viruses adapting to be more tolerant of the fever response and less virulent to their natural hosts. PMID:24750692

  9. [Aviation and space flight ophthalmology].

    PubMed

    Daumann, F J; Draeger, J

    1993-08-01

    So far it has not been possible to adapt man genetically to the specific environmental conditions of flying. A variety of disturbing factors act on the human body and affect its vital as well as sensory functions, vision being considered the most important sense for providing information. The effects of oxygen deficiency caused by increasing altitude, acceleration and centrifugal force affecting visual function, ocular motility, and pupillary reactions are well known. Like visual illusions, vibrations, high accelerative forces, high illuminance and glare at high altitudes impair the visual perception of the environment. In space flight further problems ensue from weightlessness and short-wave radiation. The high medical standards that must be met by the eyes of flying personnel, as will as for air-traffic controllers, result in the enhancement of flight safety. After operations on the cornea or retina or cataract operations in pilote, the retinal findings and DOP must be monitored closely. Special attention must be paid to means of visual aids, corrective lenses, contact lenses, and intraocular lenses. Ophthalmology is a very important element of aviation and aerospace medicine.

  10. Initial Flight Test of the Production Support Flight Control Computers at NASA Dryden Flight Research Center

    NASA Technical Reports Server (NTRS)

    Carter, John; Stephenson, Mark

    1999-01-01

    The NASA Dryden Flight Research Center has completed the initial flight test of a modified set of F/A-18 flight control computers that gives the aircraft a research control law capability. The production support flight control computers (PSFCC) provide an increased capability for flight research in the control law, handling qualities, and flight systems areas. The PSFCC feature a research flight control processor that is "piggybacked" onto the baseline F/A-18 flight control system. This research processor allows for pilot selection of research control law operation in flight. To validate flight operation, a replication of a standard F/A-18 control law was programmed into the research processor and flight-tested over a limited envelope. This paper provides a brief description of the system, summarizes the initial flight test of the PSFCC, and describes future experiments for the PSFCC.

  11. Perspectives on Highly Adaptive or Morphing Aircraft

    NASA Technical Reports Server (NTRS)

    McGowan, Anna-Maria R.; Vicroy, Dan D.; Busan, Ronald C.; Hahn, Andrew S.

    2009-01-01

    The ability to adapt to different flight conditions has been fundamental to aircraft design since the Wright Brothers first flight. Over a hundred years later, unconventional aircraft adaptability, often called aircraft morphing has become a topic of considerable renewed interest. In the past two decades, this interest has been largely fuelled by advancements in multi-functional or smart materials and structures. However, highly adaptive or morphing aircraft is certainly a cross-discipline challenge that stimulates a wide range of design possibilities. This paper will review some of the history of morphing aircraft including recent research programs and discuss some perspectives on this work.

  12. Columbia's first shakedown flight

    NASA Astrophysics Data System (ADS)

    Bell, Peter M.

    The space shuttle orbiter Columbia, first of the planned fleet of spacecraft in the nation's space transportation system, will liftoff on its first orbital shakedown flight on or about the 10th of April 1981. Launch will be from the NASA Kennedy Space Center Launch Complex 39A, no earlier than 45 minutes after sunrise. Crew for the first orbital flight will be John W. Young, commander, veteran of two Gemini and two Apollo space flights, and U.S. Navy Capt. Robert L. Crippen, pilot. Crippen has not flown in space.

  13. Flight Dynamics Analysis Branch

    NASA Technical Reports Server (NTRS)

    Stengle, Tom; Flores-Amaya, Felipe

    2000-01-01

    This report summarizes the major activities and accomplishments carried out by the Flight Dynamics Analysis Branch (FDAB), Code 572, in support of flight projects and technology development initiatives in fiscal year 2000. The report is intended to serve as a summary of the type of support carried out by the FDAB, as well as a concise reference of key accomplishments and mission experience derived from the various mission support roles. The primary focus of the FDAB is to provide expertise in the disciplines of flight dynamics, spacecraft trajectory, attitude analysis, and attitude determination and control. The FDAB currently provides support for missions and technology development projects involving NASA, government, university, and private industry.

  14. Red blood cell and iron metabolism during space flight

    NASA Technical Reports Server (NTRS)

    Smith, Scott M.

    2002-01-01

    Space flight anemia is a widely recognized phenomenon in astronauts. Reduction in circulating red blood cells and plasma volume results in a 10% to 15% decrement in circulatory volume. This effect appears to be a normal physiologic adaptation to weightlessness and results from the removal of newly released blood cells from the circulation. Iron availability increases, and (in the few subjects studied) iron stores increase during long-duration space flight. The consequences of these changes are not fully understood.

  15. MDA-9/Syntenin (SDCBP) modulates small GTPases RhoA and Cdc42 via transforming growth factor β1 to enhance epithelial-mesenchymal transition in breast cancer

    PubMed Central

    Menezes, Mitchell E.; Shen, Xue-Ning; Das, Swadesh K.; Emdad, Luni; Sarkar, Devanand; Fisher, Paul B.

    2016-01-01

    Epithelial-mesenchymal transition (EMT) is one of the decisive steps regulating cancer invasion and metastasis. However, the molecular mechanisms underlying this transition require further clarification. MDA-9/syntenin (SDCBP) expression is elevated in breast cancer patient samples as well as cultured breast cancer cells. Silencing expression of MDA-9 in mesenchymal metastatic breast cancer cells triggered a change in cell morphology in both 2D- and 3D-cultures to a more epithelial-like phenotype, along with changes in EMT markers, cytoskeletal rearrangement and decreased invasion. Conversely, over expressing MDA-9 in epithelial non-metastatic breast cancer cells instigated a change in morphology to a more mesenchymal phenotype with corresponding changes in EMT markers, cytoskeletal rearrangement and an increase in invasion. We also found that MDA-9 upregulated active levels of known modulators of EMT, the small GTPases RhoA and Cdc42, via TGFβ1. Reintroducing TGFβ1 in MDA-9 silenced cells restored active RhoA and cdc42 levels, modulated cytoskeletal rearrangement and increased invasion. We further determined that MDA-9 interacts with TGFβ1 via its PDZ1 domain. Finally, in vivo studies demonstrated that silencing the expression of MDA-9 resulted in decreased lung metastasis and TGFβ1 re-expression partially restored lung metastases. Our findings provide evidence for the relevance of MDA-9 in mediating EMT in breast cancer and support the potential of MDA-9 as a therapeutic target against metastatic disease. PMID:27863394

  16. Novel Suppressive Effects of Ketotifen on Migration and Invasion of MDA-MB-231 and HT-1080 Cancer Cells

    PubMed Central

    Kim, Hyun Ji; Park, Mi Kyung; Kim, Soo Youl; Lee, Chang Hoon

    2014-01-01

    The high mortality rates associated with cancer reflect the metastatic spread of tumor cells from the site of their origin. Metastasis, in fact, is the cause of 90% of cancer deaths. Therefore, considerable effort is being made to inhibit metastasis. In the present study, we screened ketotifen for anti-migratory and anti-invasive activities against MDA-MB-231 breast cancer and HT-1080 fibrosarcoma cancer cells. Cancer cell migration and invasion were measured using multi-well chambers. Additionally, western blots were used to examine the effects of ketotifen on the expressions of CDC42, Rho, Rac, and matrix metalloproteinase 9 (MMP-9). The results showed that ketotifen dose-dependently suppressed the migration and invasion of MDA-MB-231 and HT-1080 cells. Ketotifen also suppressed the expressions of CDC42, Rac, and Rho, which, significantly, are involved in MDA-MB-231 and HT-1080 cancer cell migration. Moreover, ketotifen suppressed the expression and activity of MMP-9, which is involved in degradation of the extracellular matrix leading to invasion. The overall data suggested that ketotifen suppresses the migration and invasion of MDA-MB-231 and HT-1080 cancer cells via inhibition of CDC42, Rac, Rho, and MMP-9 expression. PMID:25489422

  17. Splice Variants of mda-7/IL-24 Differentially Affect Survival and Induce Apoptosis in U2OS Cells

    PubMed Central

    Whitaker, Erin L.; Filippov, Valery; Filippova, Maria; Guerrero-Juarez, Christian F.; Duerksen-Hughes, Penelope J.

    2011-01-01

    Interleukin-24 (mda-7/IL-24) is a cytokine in the IL-10 family that has received a great deal of attention for its properties as a tumor suppressor and as a potential treatment for cancer. In this study, we have identified and characterized five alternatively spliced isoforms of this gene. Several, but not all of these isoforms induce apoptosis in the osteosarcoma cell line U2OS, while none affect the survival of the non-cancerous NOK cell line. One of these isoforms, lacking three exons and encoding the N-terminal end of the mda-7/IL-24 protein sequence, caused levels of apoptosis that were higher than those caused by the full-length mda-7/IL-24 variant. Additionally, we found that the ratio of isoform expression can be modified by the splice factor SRp55. This regulation suggests that alternative splicing of mda-7/IL-24 is under tight control in the cell, and can be modified under various cellular conditions, such as DNA damage. In addition to providing new insights into the function of an important tumor suppressor gene, these findings may also point toward new avenues for cancer treatment. PMID:21843952

  18. Anti-MDA5 Antibody Dermatomyositis Overlap with Systemic Lupus Erythematosus: A Case Report and Review of the Literature

    PubMed Central

    Milam, Emily C.; Futran, Jacobo; Franks Jr., Andrew G.

    2016-01-01

    Background: Dermatomyositis (DM) is an autoimmune connective tissue disease that primarily targets the muscle, skin, and lungs. Many patients have autoantibodies that correspond to distinct clinical phenotypes. Melanoma differentiation-associated gene 5 (anti-MDA5) antibody, a specific antibody that targets the melanoma differentiation-associated gene 5 (MDA5), has been reported in DM cases and is significant for a distinct cutaneous presentation and rapidly progressive interstitial lung disease. Objective: Herein, we describe a patient with DM with a positive anti-MDA5 antibody and characteristic clinical phenotype, who subsequently developed coexisting systemic lupus erythematosus (SLE). A diagnosis of SLE was supported by his clinical phenotype, positive serologies, hypocomplementemia, and progression to glomerulonephritis and lupus cerebritis, features of which fulfilled the American College of Rheumatology criteria for SLE. Conclusion: DM is known to overlap with other autoimmune diseases, including SLE, and coexistence can lead to a wide variety of clinical presentations. SLE overlapping with anti-MDA5 positive DM may present with distinct clinical features. PMID:28077979

  19. Potential suppressive effects of gentian violet on human breast cancer MDA-MB-231 cells in vitro: Comparison with gemcitabine

    PubMed Central

    Yamaguchi, Masayoshi; Murata, Tomiyasu

    2016-01-01

    Gentian violet (GV), a cationic triphenylmethane dye, is used as an antifungal and antibacterial agent. Recently, attention has been focused on GV as a potential chemotherapeutic and antiangiogenic agent. The present study was undertaken to determine the suppressive effects of GV on human breast cancer MDA-MB-231 cells in vitro. The proliferation of MDA-MB-231 cells was suppressed by culture with GV (1–200 nM). The suppressive effects of GV on cell proliferation were not potentiated in the presence of various inhibitors that induce cell cycle arrest in vitro. This finding suggested that GV inhibits G1 and G2/M phase cell cycle arrest in MDA-MB-231 cells. The suppressive effects of GV on proliferation are mediated through the inhibition of various signaling pathways or nuclear transcription in vitro. Moreover, the suppressive effects of GV on cell proliferation were compared with that of gemcitabine, a strong antitumor agent that induces nuclear DNA damage. Notably, the culture with gemcitabine >50 nM suppressed cell proliferation, while the effects of GV were observed at >1 nM. The suppressive effects of gemcitabine on cell proliferation were not potentiated by GV. Overall, the present study demonstrated that GV exhibits a potential suppressive effect on the proliferation of human breast cancer MDA-MB-231 cells in vitro. PMID:27446479

  20. Monitoring of tumor growth and metastasis potential in MDA-MB-435s/ tk-luc human breast cancer xenografts

    NASA Astrophysics Data System (ADS)

    Chang, Ya-Fang; Lin, Yi-Yu; Wang, Hsin-Ell; Liu, Ren-Shen; Pang, Fei; Hwang, Jeng-Jong

    2007-02-01

    Molecular imaging of reporter gene expression provides a rapid, sensitive and non-invasive monitoring of tumor behaviors. In this study, we reported the establishment of a novel animal model for longitudinal examination of tumor growth kinetics and metastatic spreading in vivo. The highly metastatic human breast carcinoma MDA-MB-435s cell line was engineered to stably express herpes simplex virus type 1 thymidine kinase (HSV-1- tk) and luciferase ( luc). Both 131I-FIAU and D-luciferin were used as reporter probes. For orthotopic tumor formation, MDA-MB-435s/ tk-luc cells were implanted into the first nipple of 6-week-old female NOD/SCID mice. For metastatic study, cells were injected via the lateral tail vein. Mice-bearing MDA-MB-435s/ tk-luc tumors were scanned for tumor growth and metastatsis using Xenogen IVIS50 system. Gamma scintigraphy and whole-body autoradiography were also applied to confirm the tumor localization. The results of bioluminescence imaging as well as histopathological finding showed that tumors could be detected in femur, spine, ovary, lungs, kidney, adrenal gland, lymph nodes and muscle at 16 weeks post i.v. injection, and correlated photons could be quantified. This MDA-MB-435s/ tk-luc human breast carcinoma-bearing mouse model combined with multimodalities of molecular imaging may facilitate studies on the molecular mechanisms of cancer invasion and metastasis.

  1. Optimized Method for Untargeted Metabolomics Analysis of MDA-MB-231 Breast Cancer Cells

    PubMed Central

    Peterson, Amanda L.; Walker, Adam K.; Sloan, Erica K.; Creek, Darren J.

    2016-01-01

    Cancer cells often have dysregulated metabolism, which is largely characterized by the Warburg effect—an increase in glycolytic activity at the expense of oxidative phosphorylation—and increased glutamine utilization. Modern metabolomics tools offer an efficient means to investigate metabolism in cancer cells. Currently, a number of protocols have been described for harvesting adherent cells for metabolomics analysis, but the techniques vary greatly and they lack specificity to particular cancer cell lines with diverse metabolic and structural features. Here we present an optimized method for untargeted metabolomics characterization of MDA-MB-231 triple negative breast cancer cells, which are commonly used to study metastatic breast cancer. We found that an approach that extracted all metabolites in a single step within the culture dish optimally detected both polar and non-polar metabolite classes with higher relative abundance than methods that involved removal of cells from the dish. We show that this method is highly suited to diverse applications, including the characterization of central metabolic flux by stable isotope labelling and differential analysis of cells subjected to specific pharmacological interventions. PMID:27669323

  2. PACE4 regulates proliferation, migration and invasion in human breast cancer MDA-MB-231 cells.

    PubMed

    Wang, Feifei; Wang, Lin; Pan, Jihong

    2015-01-01

    PACE4 is one of the proprotein convertases (PC) that participate in the post-translational activation of inactive proteins, leading to mature, biologically active proteins. The processing reactions occur in pairs of basic amino acids. PACE4 is an extracellular PC that binds to growth factors and several components of the extracellular matrix contributing to tumor progression. In the present study, the PACE4 gene was silenced by small interfering RNA (siRNA), and the knockdown human breast cancer MDA-MB-231 cells showed significantly reduced proliferation, migration and invasion rates. Flow cytometry analysis indicated that downregulation of PACE4 increases the percentage of cells arrested at the G0/G1 phase. Moreover, the expression of genes involved in cell growth, invasion and adhesion, i.e., IGF-2, MMP9 and MPZL2 was significantly decreased following siRNA-mediated silencing of PACE4. Taken together, these results indicate that PACE4 plays an important role in human breast cancer, and that it might represent a novel target for breast cancer therapy.

  3. Identification of atypical flight patterns

    NASA Technical Reports Server (NTRS)

    Statler, Irving C. (Inventor); Ferryman, Thomas A. (Inventor); Amidan, Brett G. (Inventor); Whitney, Paul D. (Inventor); White, Amanda M. (Inventor); Willse, Alan R. (Inventor); Cooley, Scott K. (Inventor); Jay, Joseph Griffith (Inventor); Lawrence, Robert E. (Inventor); Mosbrucker, Chris (Inventor)

    2005-01-01

    Method and system for analyzing aircraft data, including multiple selected flight parameters for a selected phase of a selected flight, and for determining when the selected phase of the selected flight is atypical, when compared with corresponding data for the same phase for other similar flights. A flight signature is computed using continuous-valued and discrete-valued flight parameters for the selected flight parameters and is optionally compared with a statistical distribution of other observed flight signatures, yielding atypicality scores for the same phase for other similar flights. A cluster analysis is optionally applied to the flight signatures to define an optimal collection of clusters. A level of atypicality for a selected flight is estimated, based upon an index associated with the cluster analysis.

  4. Energy metabolism during endurance flight and the post-flight recovery phase.

    PubMed

    Jenni-Eiermann, Susanne

    2017-02-21

    Migrating birds are known to fly non-stop for thousands of kilometres without food or water intake and at a high metabolic rate thereby relying on energy stores which were built up preceding a flight bout. Hence, from a physiological point of view the metabolism of a migrant has to switch between an active fasting phase during flight and a fuelling phase during stopover. To meet the energetic and water requirements of endurance flight, migratory birds have to store an optimal fuel composition and they have to be able to quickly mobilize and deliver sufficient energy to the working flight muscles. After flight, birds have to recover from a strenuous exercise and sleeplessness, but, at the same time, they have to be alert to escape from predators and to prepare the next flight bout. In this overview, metabolic adaptations of free-ranging migrants to both phases will be presented and compared with results from windtunnel studies. The questions whether migratory strategy (long distance versus short distance) and diet composition influence the metabolic pathways will be discussed.

  5. Integrated flight path planning system and flight control system for unmanned helicopters.

    PubMed

    Jan, Shau Shiun; Lin, Yu Hsiang

    2011-01-01

    This paper focuses on the design of an integrated navigation and guidance system for unmanned helicopters. The integrated navigation system comprises two systems: the Flight Path Planning System (FPPS) and the Flight Control System (FCS). The FPPS finds the shortest flight path by the A-Star (A*) algorithm in an adaptive manner for different flight conditions, and the FPPS can add a forbidden zone to stop the unmanned helicopter from crossing over into dangerous areas. In this paper, the FPPS computation time is reduced by the multi-resolution scheme, and the flight path quality is improved by the path smoothing methods. Meanwhile, the FCS includes the fuzzy inference systems (FISs) based on the fuzzy logic. By using expert knowledge and experience to train the FIS, the controller can operate the unmanned helicopter without dynamic models. The integrated system of the FPPS and the FCS is aimed at providing navigation and guidance to the mission destination and it is implemented by coupling the flight simulation software, X-Plane, and the computing software, MATLAB. Simulations are performed and shown in real time three-dimensional animations. Finally, the integrated system is demonstrated to work successfully in controlling the unmanned helicopter to operate in various terrains of a digital elevation model (DEM).

  6. A benchmark for fault tolerant flight control evaluation

    NASA Astrophysics Data System (ADS)

    Smaili, H.; Breeman, J.; Lombaerts, T.; Stroosma, O.

    2013-12-01

    A large transport aircraft simulation benchmark (REconfigurable COntrol for Vehicle Emergency Return - RECOVER) has been developed within the GARTEUR (Group for Aeronautical Research and Technology in Europe) Flight Mechanics Action Group 16 (FM-AG(16)) on Fault Tolerant Control (2004 2008) for the integrated evaluation of fault detection and identification (FDI) and reconfigurable flight control strategies. The benchmark includes a suitable set of assessment criteria and failure cases, based on reconstructed accident scenarios, to assess the potential of new adaptive control strategies to improve aircraft survivability. The application of reconstruction and modeling techniques, based on accident flight data, has resulted in high-fidelity nonlinear aircraft and fault models to evaluate new Fault Tolerant Flight Control (FTFC) concepts and their real-time performance to accommodate in-flight failures.

  7. Controlled Hypersonic Flight Air Data System and Flight Instrumentation

    DTIC Science & Technology

    2007-06-01

    strongly on the flight envelope, re-entry trajectory and vehicle structure. Flight envelope and re-entry trajectory influence primarily the sensor...6 3.3 Flight Wind angles and basic considerations...determination the Mach number independence principle can however be used to derive simple analytic expressions. 3.3 Flight Wind angles and basic

  8. SR-71 Flight

    NASA Video Gallery

    Two SR-71A aircraft were loaned from the U.S. Air Force for use for high-speed, high-altitude research at the NASA Dryden Flight Research Center, Edwards, California. One of them was later returned...

  9. Human Space Flight

    NASA Technical Reports Server (NTRS)

    Woolford, Barbara; Mount, Frances

    2004-01-01

    The first human space flight, in the early 1960s, was aimed primarily at determining whether humans could indeed survive and function in micro-gravity. Would eating and sleeping be possible? What mental and physical tasks could be performed? Subsequent programs increased the complexity of the tasks the crew performed. Table 1 summarizes the history of U.S. space flight, showing the projects, their dates, crew sizes, and mission durations. With over forty years of experience with human space flight, the emphasis now is on how to design space vehicles, habitats, and missions to produce the greatest returns to human knowledge. What are the roles of the humans in space flight in low earth orbit, on the moon, and in exploring Mars?

  10. Beta experiment flight report

    NASA Technical Reports Server (NTRS)

    1982-01-01

    A focused laser Doppler velocimeter system was developed for the measurement of atmospheric backscatter (beta) from aerosols at infrared wavelengths. The system was flight tested at several different locations and the results of these tests are summarized.

  11. Effects of high intensity exhaustive exercise on SOD, MDA, and NO levels in rats with knee osteoarthritis.

    PubMed

    Li, X D; Sun, G F; Zhu, W B; Wang, Y H

    2015-10-16

    The aim of this study was to investigate the impact of high intensity exhaustive exercise on nitric oxide (NO), malondialdehyde (MDA), and superoxide dismutase (SOD) expression in rats with knee osteoarthritis. Sprague Dawley rats were randomly divided into control (N = 5) and model (N = 35) groups; the model group was further divided into quiet (N = 5), low- (N = 15) and high- (N = 15) intensity exhaustive exercise groups. The low- and high-intensity groups were randomly divided into pre-exercise (N = 5), immediate post-exercise (N = 5), and 24-h post-exercise (N = 5) groups according to different time points for detection. NO, MDA, and SOD levels were compared between each group. The SOD levels in the quiet, low-, and high-intensity exhaustive exercise groups were lower than that in the control group, whereas the NO and MDA levels were higher in the former groups than in the controls (P < 0.05). The SOD level in the 24-h post-low intensity exhaustive exercise group was higher than that in the 24-h post-high intensity exhaustive exercise group, whereas the NO and MDA levels were lower in the 24-h post-low intensity than in the post-high intensity exercise group (P < 0.05). Overall, the results demonstrated that with the increase of exercise intensity, the SOD activity in the rats with knee osteoarthritis decreased gradually, whereas the MDA and NO levels gradually increased. Thus, the greater the exercise intensity, the more serious the impact on knee osteoarthritis.

  12. Human respiratory syncytial virus nucleoprotein and inclusion bodies antagonize the innate immune response mediated by MDA5 and MAVS.

    PubMed

    Lifland, Aaron W; Jung, Jeenah; Alonas, Eric; Zurla, Chiara; Crowe, James E; Santangelo, Philip J

    2012-08-01

    Currently, the spatial distribution of human respiratory syncytial virus (hRSV) proteins and RNAs in infected cells is still under investigation, with many unanswered questions regarding the interaction of virus-induced structures and the innate immune system. Very few studies of hRSV have used subcellular imaging as a means to explore the changes in localization of retinoic-acid-inducible gene-I (RIG-I)-like receptors or the mitochondrial antiviral signaling (MAVS) protein, in response to the infection and formation of viral structures. In this investigation, we found that both RIG-I and melanoma differentiation-associated gene 5 (MDA5) colocalized with viral genomic RNA and the nucleoprotein (N) as early as 6 h postinfection (hpi). By 12 hpi, MDA5 and MAVS were observed within large viral inclusion bodies (IB). We used a proximity ligation assay (PLA) and determined that the N protein was in close proximity to MDA5 and MAVS in IBs throughout the course of the infection. Similar results were found with the transient coexpression of N and the phosphoprotein (P). Additionally, we demonstrated that the localization of MDA5 and MAVS in IBs inhibited the expression of interferon β mRNA 27-fold following Newcastle disease virus infection. From these data, we concluded that the N likely interacts with MDA5, is in close proximity to MAVS, and localizes these molecules within IBs in order to attenuate the interferon response. To our knowledge, this is the first report of a specific function for hRSV IBs and of the hRSV N protein as a modulator of the innate immune response.

  13. 1999 Flight Mechanics Symposium

    NASA Technical Reports Server (NTRS)

    Lynch, John P. (Editor)

    1999-01-01

    This conference publication includes papers and abstracts presented at the Flight Mechanics Symposium held on May 18-20, 1999. Sponsored by the Guidance, Navigation and Control Center of Goddard Space Flight Center, this symposium featured technical papers on a wide range of issues related to orbit-attitude prediction, determination, and control; attitude sensor calibration; attitude determination error analysis; attitude dynamics; and orbit decay and maneuver strategy. Government, industry, and the academic community participated in the preparation and presentation of these papers.

  14. International Flight Planning Handbook.

    DTIC Science & Technology

    1985-04-01

    SAC pilots and navigators for international operations and provide increased confidence to deal effectively with contingencies that might occur. -, The...control and altitude separation. Therefore, aircrft separation is greater than operations within a radar enviro :..en:. The service is provided by the ICAO...within the CONUS ARTCC system are connected. Therefore, a handoff of a flight will be effected prior to entry into an adjoining FIR or UIR. Once a flight

  15. The flight robotics laboratory

    NASA Technical Reports Server (NTRS)

    Tobbe, Patrick A.; Williamson, Marlin J.; Glaese, John R.

    1988-01-01

    The Flight Robotics Laboratory of the Marshall Space Flight Center is described in detail. This facility, containing an eight degree of freedom manipulator, precision air bearing floor, teleoperated motion base, reconfigurable operator's console, and VAX 11/750 computer system, provides simulation capability to study human/system interactions of remote systems. The facility hardware, software and subsequent integration of these components into a real time man-in-the-loop simulation for the evaluation of spacecraft contact proximity and dynamics are described.

  16. X-29 in flight

    NASA Technical Reports Server (NTRS)

    1991-01-01

    Two X-29 aircraft, featuring one of the most unusual designs in aviation history, were flown at the NASA Ames-Dryden Flight Research Facility (later redesignated the Dryden Flight Research Center), Edwards, Calif., as technology demonstrators to investigate a host of advanced concepts and technologies. This 23 second clip begins with a camera pan from the aircraft's right rear quarter forward as the X-29 flies along in a near- stall maneuver.

  17. [Biomedical results of the Space Shuttle Orbital Flight Test Program].

    PubMed

    Pool, S L; Nicogossian, A

    1984-01-01

    On July 4, 1982 the Space Shuttle Columbia landed at Edwards Air Force Base, California, thus successfully completing the fourth and last in a series of Orbital Flight Tests (OFT) of the Space Transportation System (STS). The primary goal of medical operation support for the OFT was to assure the health and well-being of flight personnel during all phases of the mission. To this end, the crew health status was evaluated preflight, inflight and postflight. Biomedical flight test requirements were completed in the following areas: physiological adaptation to microgravity, cabin acoustical noise, cabin atmospheric evaluation, radiation dosimetry, crew exercise equipment evaluation and a cardiovascular deconditioning countermeasure assessment.

  18. Biomedical results of the Space Shuttle orbital flight test program

    NASA Technical Reports Server (NTRS)

    Pool, S. L.; Nicogossian, A.

    1983-01-01

    On July 4, 1982, the Space Shuttle Columbia landed at Edwards Air Force Base, CA, thus successfully completing the fourth and last in a series of Orbital Flight Tests (OFT) of the Space Transportation System (STS). The primary goal of medical operations support for the OFT was to assure the health and well-being of flight personnel during all phases of the mission. To this end, crew health status was evaluated preflight, inflight, and postflight. Biomedical flight test requirements were completed in the following areas: physiological adaptation to microgravity, cabin acoustical noise, cabin atmospheric evaluation, radiation dosimetry, crew exercise equipment evaluation, and a cardiovascular deconditioning countermeasure assessment.

  19. Rapid perceptual adaptation to high gravitoinertial force levels Evidence for context-specific adaptation

    NASA Technical Reports Server (NTRS)

    Lackner, J. R.; Graybiel, A.

    1982-01-01

    Subjects exposed to periodic variations in gravitoinertial force (2-G peak) in parabolic flight maneuvers quickly come to perceive the peak force level as having decreased in intensity. By the end of a 40-parabola flight, the decrease in apparent force is approximately 40%. On successive flight days, the apparent intensity of the force loads seems to decrease as well, indicating a cumulative adaptive effect. None of the subjects reported feeling abnormally 'light' for more than a minute or two after return to 1-G background force levels. The pattern of findings suggests a context-specific adaptation to high-force levels.

  20. Magnesium and Space Flight

    PubMed Central

    Smith, Scott M.; Zwart, Sara R.

    2015-01-01

    Magnesium is an essential nutrient for muscle, cardiovascular, and bone health on Earth, and during space flight. We sought to evaluate magnesium status in 43 astronauts (34 male, 9 female; 47 ± 5 years old, mean ± SD) before, during, and after 4–6-month space missions. We also studied individuals participating in a ground analog of space flight (head-down-tilt bed rest; n = 27 (17 male, 10 female), 35 ± 7 years old). We evaluated serum concentration and 24-h urinary excretion of magnesium, along with estimates of tissue magnesium status from sublingual cells. Serum magnesium increased late in flight, while urinary magnesium excretion was higher over the course of 180-day space missions. Urinary magnesium increased during flight but decreased significantly at landing. Neither serum nor urinary magnesium changed during bed rest. For flight and bed rest, significant correlations existed between the area under the curve of serum and urinary magnesium and the change in total body bone mineral content. Tissue magnesium concentration was unchanged after flight and bed rest. Increased excretion of magnesium is likely partially from bone and partially from diet, but importantly, it does not come at the expense of muscle tissue stores. While further study is needed to better understand the implications of these findings for longer space exploration missions, magnesium homeostasis and tissue status seem well maintained during 4–6-month space missions. PMID:26670248

  1. Magnesium and Space Flight.

    PubMed

    Smith, Scott M; Zwart, Sara R

    2015-12-08

    Magnesium is an essential nutrient for muscle, cardiovascular, and bone health on Earth, and during space flight. We sought to evaluate magnesium status in 43 astronauts (34 male, 9 female; 47 ± 5 years old, mean ± SD) before, during, and after 4-6-month space missions. We also studied individuals participating in a ground analog of space flight (head-down-tilt bed rest; n = 27 (17 male, 10 female), 35 ± 7 years old). We evaluated serum concentration and 24-h urinary excretion of magnesium, along with estimates of tissue magnesium status from sublingual cells. Serum magnesium increased late in flight, while urinary magnesium excretion was higher over the course of 180-day space missions. Urinary magnesium increased during flight but decreased significantly at landing. Neither serum nor urinary magnesium changed during bed rest. For flight and bed rest, significant correlations existed between the area under the curve of serum and urinary magnesium and the change in total body bone mineral content. Tissue magnesium concentration was unchanged after flight and bed rest. Increased excretion of magnesium is likely partially from bone and partially from diet, but importantly, it does not come at the expense of muscle tissue stores. While further study is needed to better understand the implications of these findings for longer space exploration missions, magnesium homeostasis and tissue status seem well maintained during 4-6-month space missions.

  2. Designing Flight Deck Procedures

    NASA Technical Reports Server (NTRS)

    Degani, Asaf; Wiener, Earl

    2005-01-01

    Three reports address the design of flight-deck procedures and various aspects of human interaction with cockpit systems that have direct impact on flight safety. One report, On the Typography of Flight- Deck Documentation, discusses basic research about typography and the kind of information needed by designers of flight deck documentation. Flight crews reading poorly designed documentation may easily overlook a crucial item on the checklist. The report surveys and summarizes the available literature regarding the design and typographical aspects of printed material. It focuses on typographical factors such as proper typefaces, character height, use of lower- and upper-case characters, line length, and spacing. Graphical aspects such as layout, color coding, fonts, and character contrast are discussed; and several cockpit conditions such as lighting levels and glare are addressed, as well as usage factors such as angular alignment, paper quality, and colors. Most of the insights and recommendations discussed in this report are transferable to paperless cockpit systems of the future and computer-based procedure displays (e.g., "electronic flight bag") in aerospace systems and similar systems that are used in other industries such as medical, nuclear systems, maritime operations, and military systems.

  3. Visual Adaptation

    PubMed Central

    Webster, Michael A.

    2015-01-01

    Sensory systems continuously mold themselves to the widely varying contexts in which they must operate. Studies of these adaptations have played a long and central role in vision science. In part this is because the specific adaptations remain a powerful tool for dissecting vision, by exposing the mechanisms that are adapting. That is, “if it adapts, it's there.” Many insights about vision have come from using adaptation in this way, as a method. A second important trend has been the realization that the processes of adaptation are themselves essential to how vision works, and thus are likely to operate at all levels. That is, “if it's there, it adapts.” This has focused interest on the mechanisms of adaptation as the target rather than the probe. Together both approaches have led to an emerging insight of adaptation as a fundamental and ubiquitous coding strategy impacting all aspects of how we see. PMID:26858985

  4. Neural Networks for Flight Control

    NASA Technical Reports Server (NTRS)

    Jorgensen, Charles C.

    1996-01-01

    Neural networks are being developed at NASA Ames Research Center to permit real-time adaptive control of time varying nonlinear systems, enhance the fault-tolerance of mission hardware, and permit online system reconfiguration. In general, the problem of controlling time varying nonlinear systems with unknown structures has not been solved. Adaptive neural control techniques show considerable promise and are being applied to technical challenges including automated docking of spacecraft, dynamic balancing of the space station centrifuge, online reconfiguration of damaged aircraft, and reducing cost of new air and spacecraft designs. Our experiences have shown that neural network algorithms solved certain problems that conventional control methods have been unable to effectively address. These include damage mitigation in nonlinear reconfiguration flight control, early performance estimation of new aircraft designs, compensation for damaged planetary mission hardware by using redundant manipulator capability, and space sensor platform stabilization. This presentation explored these developments in the context of neural network control theory. The discussion began with an overview of why neural control has proven attractive for NASA application domains. The more important issues in control system development were then discussed with references to significant technical advances in the literature. Examples of how these methods have been applied were given, followed by projections of emerging application needs and directions.

  5. 14 CFR 121.414 - Initial and transition training and checking requirements: flight instructors (airplane), flight...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ....414 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED... flight training for flight instructors (airplane), flight engineer instructors (airplane), and flight... to develop during instruction. (4) For flight engineer instructors (airplane) and flight...

  6. 14 CFR 121.414 - Initial and transition training and checking requirements: flight instructors (airplane), flight...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ....414 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED... flight training for flight instructors (airplane), flight engineer instructors (airplane), and flight... to develop during instruction. (4) For flight engineer instructors (airplane) and flight...

  7. 14 CFR 121.414 - Initial and transition training and checking requirements: flight instructors (airplane), flight...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ....414 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED... flight training for flight instructors (airplane), flight engineer instructors (airplane), and flight... to develop during instruction. (4) For flight engineer instructors (airplane) and flight...

  8. Mission Adaptive Wing test program

    NASA Technical Reports Server (NTRS)

    Birk, Frank T.; Smith, Rogers E.

    1986-01-01

    With the completion of the F-111 test-bed Mission Adaptive Wing (MAW) test program's manual flight control system, emphasis has been shifted to flight testing of MAW automatic control modes. These encompass (1) cruise camber control, (2) maneuver camber control, (3) maneuver load control, and (4) maneuver enhancement and load alleviation control. The aircraft is currently cleared to a 2.5-g maneuvering limit due to generally higher variable-incidence wing pivot loads than had been anticipated, especially at the higher wing-camber settings. Buffet is noted to be somewhat higher than expected at the higher camber settings.

  9. Effect of light intensity on flight control and temporal properties of photoreceptors in bumblebees.

    PubMed

    Reber, Therese; Vähäkainu, Antti; Baird, Emily; Weckström, Matti; Warrant, Eric; Dacke, Marie

    2015-05-01

    To control flight, insects rely on the pattern of visual motion generated on the retina as they move through the environment. When light levels fall, vision becomes less reliable and flight control thus becomes more challenging. Here, we investigated the effect of light intensity on flight control by filming the trajectories of free-flying bumblebees (Bombus terrestris, Linnaeus 1758) in an experimental tunnel at different light levels. As light levels fell, flight speed decreased and the flight trajectories became more tortuous but the bees were still remarkably good at centring their flight about the tunnel's midline. To investigate whether this robust flight performance can be explained by visual adaptations in the bumblebee retina, we also examined the response speed of the green-sensitive photoreceptors at the same light intensities. We found that the response speed of the photoreceptors significantly decreased as light levels fell. This indicates that bumblebees have both behavioural (reduction in flight speed) and retinal (reduction in response speed of the photoreceptors) adaptations to allow them to fly in dim light. However, the more tortuous flight paths recorded in dim light suggest that these adaptations do not support flight with the same precision during the twilight hours of the day.

  10. Morphological and kinematic basis of the hummingbird flight stroke: scaling of flight muscle transmission ratio.

    PubMed

    Hedrick, Tyson L; Tobalske, Bret W; Ros, Ivo G; Warrick, Douglas R; Biewener, Andrew A

    2012-05-22

    Hummingbirds (Trochilidae) are widely known for their insect-like flight strokes characterized by high wing beat frequency, small muscle strains and a highly supinated wing orientation during upstroke that allows for lift production in both halves of the stroke cycle. Here, we show that hummingbirds achieve these functional traits within the limits imposed by a vertebrate endoskeleton and muscle physiology by accentuating a wing inversion mechanism found in other birds and using long-axis rotational movement of the humerus. In hummingbirds, long-axis rotation of the humerus creates additional wing translational movement, supplementing that produced by the humeral elevation and depression movements of a typical avian flight stroke. This adaptation increases the wing-to-muscle-transmission ratio, and is emblematic of a widespread scaling trend among flying animals whereby wing-to-muscle-transmission ratio varies inversely with mass, allowing animals of vastly different sizes to accommodate aerodynamic, biomechanical and physiological constraints on muscle-powered flapping flight.

  11. Adaptive control with aerospace applications

    NASA Astrophysics Data System (ADS)

    Gadient, Ross

    Robust and adaptive control techniques have a rich history of theoretical development with successful application. Despite the accomplishments made, attempts to combine the best elements of each approach into robust adaptive systems has proven challenging, particularly in the area of application to real world aerospace systems. In this research, we investigate design methods for general classes of systems that may be applied to representative aerospace dynamics. By combining robust baseline control design with augmentation designs, our work aims to leverage the advantages of each approach. This research contributes the development of robust model-based control design for two classes of dynamics: 2nd order cascaded systems, and a more general MIMO framework. We present a theoretically justified method for state limiting via augmentation of a robust baseline control design. Through the development of adaptive augmentation designs, we are able to retain system performance in the presence of uncertainties. We include an extension that combines robust baseline design with both state limiting and adaptive augmentations. In addition we develop an adaptive augmentation design approach for a class of dynamic input uncertainties. We present formal stability proofs and analyses for all proposed designs in the research. Throughout the work, we present real world aerospace applications using relevant flight dynamics and flight test results. We derive robust baseline control designs with application to both piloted and unpiloted aerospace system. Using our developed methods, we add a flight envelope protecting state limiting augmentation for piloted aircraft applications and demonstrate the efficacy of our approach via both simulation and flight test. We illustrate our adaptive augmentation designs via application to relevant fixed-wing aircraft dynamics. Both a piloted example combining the state limiting and adaptive augmentation approaches, and an unpiloted example with

  12. Parallel methods for the flight simulation model

    SciTech Connect

    Xiong, Wei Zhong; Swietlik, C.

    1994-06-01

    The Advanced Computer Applications Center (ACAC) has been involved in evaluating advanced parallel architecture computers and the applicability of these machines to computer simulation models. The advanced systems investigated include parallel machines with shared. memory and distributed architectures consisting of an eight processor Alliant FX/8, a twenty four processor sor Sequent Symmetry, Cray XMP, IBM RISC 6000 model 550, and the Intel Touchstone eight processor Gamma and 512 processor Delta machines. Since parallelizing a truly efficient application program for the parallel machine is a difficult task, the implementation for these machines in a realistic setting has been largely overlooked. The ACAC has developed considerable expertise in optimizing and parallelizing application models on a collection of advanced multiprocessor systems. One of aspect of such an application model is the Flight Simulation Model, which used a set of differential equations to describe the flight characteristics of a launched missile by means of a trajectory. The Flight Simulation Model was written in the FORTRAN language with approximately 29,000 lines of source code. Depending on the number of trajectories, the computation can require several hours to full day of CPU time on DEC/VAX 8650 system. There is an impetus to reduce the execution time and utilize the advanced parallel architecture computing environment available. ACAC researchers developed a parallel method that allows the Flight Simulation Model to be able to run in parallel on the multiprocessor system. For the benchmark data tested, the parallel Flight Simulation Model implemented on the Alliant FX/8 has achieved nearly linear speedup. In this paper, we describe a parallel method for the Flight Simulation Model. We believe the method presented in this paper provides a general concept for the design of parallel applications. This concept, in most cases, can be adapted to many other sequential application programs.

  13. Flight Planning in the Cloud

    NASA Technical Reports Server (NTRS)

    Flores, Sarah L.; Chapman, Bruce D.; Tung, Waye W.; Zheng, Yang

    2011-01-01

    This new interface will enable Principal Investigators (PIs), as well as UAVSAR (Uninhabited Aerial Vehicle Synthetic Aperture Radar) members to do their own flight planning and time estimation without having to request flight lines through the science coordinator. It uses an all-in-one Google Maps interface, a JPL hosted database, and PI flight requirements to design an airborne flight plan. The application will enable users to see their own flight plan being constructed interactively through a map interface, and then the flight planning software will generate all the files necessary for the flight. Afterward, the UAVSAR team can then complete the flight request, including calendaring and supplying requisite flight request files in the expected format for processing by NASA s airborne science program. Some of the main features of the interface include drawing flight lines on the map, nudging them, adding them to the current flight plan, and reordering them. The user can also search and select takeoff, landing, and intermediate airports. As the flight plan is constructed, all of its components are constantly being saved to the database, and the estimated flight times are updated. Another feature is the ability to import flight lines from previously saved flight plans. One of the main motivations was to make this Web application as simple and intuitive as possible, while also being dynamic and robust. This Web application can easily be extended to support other airborne instruments.

  14. Modification of Otolith Reflex Asymmetries Following Space Flight

    NASA Technical Reports Server (NTRS)

    Clarke, Andrew H.; Schoenfeld, Uwe; Wood, Scott J.

    2011-01-01

    We hypothesize that changes in otolith-mediated reflexes adapted for microgravity contribute to perceptual, gaze and postural disturbances upon return to Earth s gravity. Our goal was to determine pre- versus post-fight differences in unilateral otolith reflexes that reflect these adaptive changes. This study represents the first comprehensive examination of unilateral otolith function following space flight. Ten astronauts participated in unilateral otolith function tests three times pre-flight and up to four times after Shuttle flights from landing day through the subsequent 10 days. During unilateral centrifugation (UC, +/- 3.5cm at 400deg/s), utricular function was examined by the perceptual changes reflected by the subjective visual vertical (SVV) and by video-oculographic measurement of the otolith-mediated ocular counter-roll (OOR). Unilateral saccular reflexes were recorded by measurement of collic Vestibular Evoked Myogenic Potential (cVEMP). Although data from a few subjects were not obtained early post-flight, a general increase in asymmetry of otolith responses was observed on landing day relative to pre-flight baseline, with a subsequent reversal in asymmetry within 2-3 days. Recovery to baseline levels was achieved within 10 days. This fluctuation in the asymmetry measures appeared strongest for SVV, in a consistent direction for OOR, and in an opposite direction for cVEMP. These results are consistent with our hypothesis that space flight results in adaptive changes in central nervous system processing of otolith input. Adaptation to microgravity may reveal asymmetries in otolith function upon to return to Earth that were not detected prior to the flight due to compensatory mechanisms.

  15. Long duration flights management

    NASA Astrophysics Data System (ADS)

    Sosa-Sesma, Sergio; Letrenne, Gérard; Spel, Martin; Charbonnier, Jean-Marc

    Long duration flights (LDF) require a special management to take the best decisions in terms of ballast consumption and instant of separation. As a contrast to short duration flights, where meteorological conditions are relatively well known, for LDF we need to include the meteorological model accuracy in trajectory simulations. Dispersions on the fields of model (wind, temperature and IR fluxes) could make the mission incompatible with safety rules, authorized zones and others flight requirements. Last CNES developments for LDF act on three main axes: 1. Although ECMWF-NCEP forecast allows generating simulations from a 4D point (altitude, latitude, longitude and UT time), result is not statistical, it is determinist. To take into account model dispersion a meteorological NCEP data base was analyzed. A comparison between Analysis (AN) and Forecast (FC) for the same time frame had been done. Result obtained from this work allows implementing wind and temperature dispersions on balloon flight simulator. 2. For IR fluxes, NCEP does not provide ascending IR fluxes in AN mode but only in FC mode. To obtain the IR fluxes for each time frame, satellite images are used. A comparison between FC and satellites measurements had been done. Results obtained from this work allow implementing flux dispersions on balloon flight simulator. 3. An improved cartography containing a vast data base had been included in balloon flight simulator. Mixing these three points with balloon flight dynamics we have obtained two new tools for observing balloon evolution and risk, one of them is called ASTERISK (Statistic Tool for Evaluation of Risk) for calculations and the other one is called OBERISK (Observing Balloon Evolution and Risk) for visualization. Depending on the balloon type (super pressure, zero pressure or MIR) relevant information for the flight manager is different. The goal is to take the best decision according to the global situation to obtain the largest flight duration with

  16. XV-15 in flight

    NASA Technical Reports Server (NTRS)

    1981-01-01

    The XV-15 Tilt-Rotor aircraft was designed by Bell Aircraft, Niagara Falls, New York, in the mid-1970's under a contract with NASA and the U.S. Army. It was capable of taking off and landing vertically like a helicopter and of flying horizontally when its 'prop rotors' were rotated forward and downward. NASA Ames Research Center, Mountain View, California, and the Army Air Mobility Laboratory cooperated in a program to obtain two of the aircraft for flight research. The first aircraft arrived at Ames on March 23, 1978. After wind-tunnel testing in the Ames 40-by-80-foot wind tunnel, the aircraft began its contractor flight tests at Ames on April 23, 1979. Bell, Army, and U.S. Marine pilots flew it on 140 separate missions over the next year before turning the aircraft over to Ames. That center, in turn, chose to perform the initial flight research at the Dryden Flight Research Center, Edwards, California, where aircraft Number 2 began flight research with Dryden pilots on October 3, 1980, followed by aircraft Number 1 (previously the wind-tunnel model) the following year. Service pilots continued to fly the aircraft, including missions at Fort Huachuca, Arizona, and aboard the Navy USS Tripoli. Ames pilots also flew the XV-15 extensively during its lengthy period of flight research. The Ames flight research team finally returned aircraft Number 2 to Bell Helicopter in April 1994. The successful flight research with the XV-15, spearheaded by the team at Ames, led to the military V-22 Osprey and to the possibility of using tilt-rotor aircraft as a solution to the problem of crowded airports and highways. The XV-15 weighed 9,076 pounds empty and measured slightly more than 46 feet in length. The distance from the ground to the top of the tail was nearly 13 feet, and the span of its forward-swept wings was about 32 feet. It featured two three-bladed rotors, each measuring 25 feet in diameter. This movie clip runs about 49 seconds showing the XV-15 aircraft turning and

  17. Overexpression of HER-2 in MDA-MB-435/LCC6 Tumours is Associated with Higher Metabolic Activity and Lower Energy Stress

    PubMed Central

    Dragowska, Wieslawa H.; Ginj, Mihaela; Kozlowski, Piotr; Yung, Andrew; Ruth, Thomas J.; Adam, Michael J.; Sossi, Vesna; Bally, Marcel B.; Yapp, Donald T. T.

    2016-01-01

    Overexpresssion of HER-2 in the MDA-MB-435/LCC6 (LCC6HER-2) tumour model is associated with significantly increased hypoxia and reduced necrosis compared to isogenic control tumours (LCC6Vector); this difference was not related to tumour size or changes in vascular architecture. To further evaluate factors responsible for HER-2-associated changes in the tumour microenvironment, small animal magnetic resonance imaging (MRI) and positron emission tomography (PET) were used to measure tumour tissue perfusion and metabolism, respectively. The imaging data was further corroborated by analysis of molecular markers pertaining to energy homeostasis, and measurements of hypoxia and glucose consumption. The results showed a strong trend towards higher perfusion rates (~58% greater, p = 0.14), and significantly higher glucose uptake in LCC6HER-2 (~2-fold greater; p = 0.025), relative to control tumours. The expression of proteins related to energy stress (P-AMPK, P-ACC) and glucose transporters (GLUT1) were lower in LCC6HER-2 tumours (~2- and ~4-fold, respectively). The in vitro analysis showed that LCC6HER-2 cells become more hypoxic in 1% oxygen and utilise significantly more glucose in normoxia compared to LCC6Vectorcells (p < 0.005). Amalgamation of all the data points suggests a novel metabolic adaptation driven by HER-2 overexpression where higher oxygen and glucose metabolic rates produce rich energy supply but also a more hypoxic tumour mass. PMID:26727049

  18. Adaptive Management

    EPA Science Inventory

    Adaptive management is an approach to natural resource management that emphasizes learning through management where knowledge is incomplete, and when, despite inherent uncertainty, managers and policymakers must act. Unlike a traditional trial and error approach, adaptive managem...

  19. Aircraft as adaptive nonlinear system which must be in the adaptational maximum zone for safety

    SciTech Connect

    Ignative, M.; Simatos, N.; Sivasundaram, S.

    1994-12-31

    Safety is a main problem in aircraft. We are considering this problem from the point of view related to existence of the adaptational maximum in complex developing systems. Safety space of aircraft parameters are determined. This space is transformed to different regimes of flight, when one engine malfunctions etc., are considered. Also it is shown that maximum safety is in adaptational maximum zone.

  20. Eclipse takeoff and flight

    NASA Technical Reports Server (NTRS)

    1998-01-01

    This 25-second clip shows the QF-106 'Delta Dart' tethered to the USAF C-141A during takeoff and in flight. NASA Dryden Flight Research Center, Edwards, California, supported a Kelly Space and Technology, Inc. (KST)/U.S. Air Force project known as Eclipse, which demonstrated a reusable tow launch vehicle concept. The purpose of the project was to demonstrate a reusable tow launch vehicle concept that had been conceived and patented by KST. Kelly Space obtained a contract with the USAF Research Laboratory for the tow launch demonstration project under the Small Business Innovation Research (SBIR) program. The USAF SBIR contract included the modifications to turn the QF-106 into the Experimental Demonstrator #1 (EXD-01), and the C141A aircraft to incorporate the tow provisions to link the two aircraft, as well as conducting flight tests. The demonstration consisted of ground and flight tests. These tests included a Combined Systems Test of both airplanes joined by a tow rope, a towed taxi test, and six towed flights. The primary goal of the project was demonstrating the tow phase of the Eclipse concept using a scaled-down tow aircraft (C-141A) and a representative aerodynamically-shaped aircraft (QF-106A) as a launch vehicle. This was successfully accomplished. On December 20, 1997, NASA research pilot Mark Stucky flew a QF-106 on the first towed flight behind an Air Force C-141 in the joint Eclipse project with KST to demonstrate the reusable tow launch vehicle concept developed by KST. Kelly hoped to use the data from the tow tests to validate a tow-to-launch procedure for reusable space launch vehicles. Stucky flew six successful tow tests between December 1997 and February 6, 1998. On February 6, 1998, the sixth and final towed flight brought the project to a successful completion. Preliminary flight results determined that the handling qualities of the QF-106 on tow were very stable; actual flight measured values of tow rope tension were well within predictions

  1. Perseus Post-flight

    NASA Technical Reports Server (NTRS)

    1991-01-01

    Crew members check out the Perseus proof-of-concept vehicle on Rogers Dry Lake, adjacent to the Dryden Flight Research Center, Edwards, California, after a test flight in 1991. Perseus B is a remotely piloted aircraft developed as a design-performance testbed under NASA's Environmental Research Aircraft and Sensor Technology (ERAST) project. Perseus is one of several flight vehicles involved in the ERAST project. A piston engine, propeller-powered aircraft, Perseus was designed and built by Aurora Flight Sciences Corporation, Manassas, Virginia. The objectives of Perseus B's ERAST flight tests have been to reach and maintain horizontal flight above altitudes of 60,000 feet and demonstrate the capability to fly missions lasting from 8 to 24 hours, depending on payload and altitude requirements. The Perseus B aircraft established an unofficial altitude record for a single-engine, propeller-driven, remotely piloted aircraft on June 27, 1998. It reached an altitude of 60,280 feet. In 1999, several modifications were made to the Perseus aircraft including engine, avionics, and flight-control-system improvements. These improvements were evaluated in a series of operational readiness and test missions at the Dryden Flight Research Center, Edwards, California. Perseus is a high-wing monoplane with a conventional tail design. Its narrow, straight, high-aspect-ratio wing is mounted atop the fuselage. The aircraft is pusher-designed with the propeller mounted in the rear. This design allows for interchangeable scientific-instrument payloads to be placed in the forward fuselage. The design also allows for unobstructed airflow to the sensors and other devices mounted in the payload compartment. The Perseus B that underwent test and development in 1999 was the third generation of the Perseus design, which began with the Perseus Proof-Of-Concept aircraft. Perseus was initially developed as part of NASA's Small High-Altitude Science Aircraft (SHASA) program, which later evolved

  2. Perseus in Flight

    NASA Technical Reports Server (NTRS)

    1991-01-01

    The Perseus proof-of-concept vehicle in flight at the Dryden Flight Research Center, Edwards, California in 1991. Perseus is one of several remotely-piloted aircraft designed for high-altitude, long-endurance scientific sampling missions being evaluated under the ERAST program. Perseus B is a remotely piloted aircraft developed as a design-performance testbed under NASA's Environmental Research Aircraft and Sensor Technology (ERAST) project. Perseus is one of several flight vehicles involved in the ERAST project. A piston engine, propeller-powered aircraft, Perseus was designed and built by Aurora Flight Sciences Corporation, Manassas, Virginia. The objectives of Perseus B's ERAST flight tests have been to reach and maintain horizontal flight above altitudes of 60,000 feet and demonstrate the capability to fly missions lasting from 8 to 24 hours, depending on payload and altitude requirements. The Perseus B aircraft established an unofficial altitude record for a single-engine, propeller-driven, remotely piloted aircraft on June 27, 1998. It reached an altitude of 60,280 feet. In 1999, several modifications were made to the Perseus aircraft including engine, avionics, and flight-control-system improvements. These improvements were evaluated in a series of operational readiness and test missions at the Dryden Flight Research Center, Edwards, California. Perseus is a high-wing monoplane with a conventional tail design. Its narrow, straight, high-aspect-ratio wing is mounted atop the fuselage. The aircraft is pusher-designed with the propeller mounted in the rear. This design allows for interchangeable scientific-instrument payloads to be placed in the forward fuselage. The design also allows for unobstructed airflow to the sensors and other devices mounted in the payload compartment. The Perseus B that underwent test and development in 1999 was the third generation of the Perseus design, which began with the Perseus Proof-Of-Concept aircraft. Perseus was initially

  3. Aerodynamics of bird flight

    NASA Astrophysics Data System (ADS)

    Dvořák, Rudolf

    2016-03-01

    Unlike airplanes birds must have either flapping or oscillating wings (the hummingbird). Only such wings can produce both lift and thrust - two sine qua non attributes of flying.The bird wings have several possibilities how to obtain the same functions as airplane wings. All are realized by the system of flight feathers. Birds have also the capabilities of adjusting the shape of the wing according to what the immediate flight situation demands, as well as of responding almost immediately to conditions the flow environment dictates, such as wind gusts, object avoidance, target tracking, etc. In bird aerodynamics also the tail plays an important role. To fly, wings impart downward momentum to the surrounding air and obtain lift by reaction. How this is achieved under various flight situations (cruise flight, hovering, landing, etc.), and what the role is of the wing-generated vortices in producing lift and thrust is discussed.The issue of studying bird flight experimentally from in vivo or in vitro experiments is also briefly discussed.

  4. Airplane tracking documents the fastest flight speeds recorded for bats

    PubMed Central

    Kunz, Thomas H.; Dechmann, Dina K. N.; Swartz, Sharon M.; Wikelski, Martin

    2016-01-01

    The performance capabilities of flying animals reflect the interplay of biomechanical and physiological constraints and evolutionary innovation. Of the two extant groups of vertebrates that are capable of powered flight, birds are thought to fly more efficiently and faster than bats. However, fast-flying bat species that are adapted for flight in open airspace are similar in wing shape and appear to be similar in flight dynamics to fast-flying birds that exploit the same aerial niche. Here, we investigate flight behaviour in seven free-flying Brazilian free-tailed bats (Tadarida brasiliensis) and report that the maximum ground speeds achieved exceed speeds previously documented for any bat. Regional wind modelling indicates that bats adjusted flight speeds in response to winds by flying more slowly as wind support increased and flying faster when confronted with crosswinds, as demonstrated for insects, birds and other bats. Increased frequency of pauses in wing beats at faster speeds suggests that flap-gliding assists the bats' rapid flight. Our results suggest that flight performance in bats has been underappreciated and that functional differences in the flight abilities of birds and bats require re-evaluation. PMID:28018618

  5. Airplane tracking documents the fastest flight speeds recorded for bats.

    PubMed

    McCracken, Gary F; Safi, Kamran; Kunz, Thomas H; Dechmann, Dina K N; Swartz, Sharon M; Wikelski, Martin

    2016-11-01

    The performance capabilities of flying animals reflect the interplay of biomechanical and physiological constraints and evolutionary innovation. Of the two extant groups of vertebrates that are capable of powered flight, birds are thought to fly more efficiently and faster than bats. However, fast-flying bat species that are adapted for flight in open airspace are similar in wing shape and appear to be similar in flight dynamics to fast-flying birds that exploit the same aerial niche. Here, we investigate flight behaviour in seven free-flying Brazilian free-tailed bats (Tadarida brasiliensis) and report that the maximum ground speeds achieved exceed speeds previously documented for any bat. Regional wind modelling indicates that bats adjusted flight speeds in response to winds by flying more slowly as wind support increased and flying faster when confronted with crosswinds, as demonstrated for insects, birds and other bats. Increased frequency of pauses in wing beats at faster speeds suggests that flap-gliding assists the bats' rapid flight. Our results suggest that flight performance in bats has been underappreciated and that functional differences in the flight abilities of birds and bats require re-evaluation.

  6. Remotely Piloted Vehicles for Experimental Flight Control Testing

    NASA Technical Reports Server (NTRS)

    Motter, Mark A.; High, James W.

    2009-01-01

    A successful flight test and training campaign of the NASA Flying Controls Testbed was conducted at Naval Outlying Field, Webster Field, MD during 2008. Both the prop and jet-powered versions of the subscale, remotely piloted testbeds were used to test representative experimental flight controllers. These testbeds were developed by the Subsonic Fixed Wing Project s emphasis on new flight test techniques. The Subsonic Fixed Wing Project is under the Fundamental Aeronautics Program of NASA's Aeronautics Research Mission Directorate (ARMD). The purpose of these testbeds is to quickly and inexpensively evaluate advanced concepts and experimental flight controls, with applications to adaptive control, system identification, novel control effectors, correlation of subscale flight tests with wind tunnel results, and autonomous operations. Flight tests and operator training were conducted during four separate series of tests during April, May, June and August 2008. Experimental controllers were engaged and disengaged during fully autonomous flight in the designated test area. Flaps and landing gear were deployed by commands from the ground control station as unanticipated disturbances. The flight tests were performed NASA personnel with support from the Maritime Unmanned Development and Operations (MUDO) team of the Naval Air Warfare Center, Aircraft Division

  7. Space Launch System Ascent Flight Control Design

    NASA Technical Reports Server (NTRS)

    VanZwieten, Tannen S.; Orr, Jeb S.; Wall, John H.; Hall, Charles E.

    2014-01-01

    A robust and flexible autopilot architecture for NASA's Space Launch System (SLS) family of launch vehicles is presented. As the SLS configurations represent a potentially significant increase in complexity and performance capability of the integrated flight vehicle, it was recognized early in the program that a new, generalized autopilot design should be formulated to fulfill the needs of this new space launch architecture. The present design concept is intended to leverage existing NASA and industry launch vehicle design experience and maintain the extensibility and modularity necessary to accommodate multiple vehicle configurations while relying on proven and flight-tested control design principles for large boost vehicles. The SLS flight control architecture combines a digital three-axis autopilot with traditional bending filters to support robust active or passive stabilization of the vehicle's bending and sloshing dynamics using optimally blended measurements from multiple rate gyros on the vehicle structure. The algorithm also relies on a pseudo-optimal control allocation scheme to maximize the performance capability of multiple vectored engines while accommodating throttling and engine failure contingencies in real time with negligible impact to stability characteristics. The architecture supports active in-flight load relief through the use of a nonlinear observer driven by acceleration measurements, and envelope expansion and robustness enhancement is obtained through the use of a multiplicative forward gain modulation law based upon a simple model reference adaptive control scheme.

  8. Numerical study of insect free hovering flight

    NASA Astrophysics Data System (ADS)

    Wu, Di; Yeo, Khoon Seng; Lim, Tee Tai; Fluid lab, Mechanical Engineering, National University of Singapore Team

    2012-11-01

    In this paper we present the computational fluid dynamics study of three-dimensional flow field around a free hovering fruit fly integrated with unsteady FSI analysis and the adaptive flight control system for the first time. The FSI model being specified for fruitfly hovering is achieved by coupling a structural problem based on Newton's second law with a rigorous CFD solver concerning generalized finite difference method. In contrast to the previous hovering flight research, the wing motion employed here is not acquired from experimental data but governed by our proposed control systems. Two types of hovering control strategies i.e. stroke plane adjustment mode and paddling mode are explored, capable of generating the fixed body position and orientation characteristic of hovering flight. Hovering flight associated with multiple wing kinematics and body orientations are shown as well, indicating the means by which fruitfly actually maintains hovering may have considerable freedom and therefore might be influenced by many other factors beyond the physical and aerodynamic requirements. Additionally, both the near- and far-field flow and vortex structure agree well with the results from other researchers, demonstrating the reliability of our current model.

  9. Perseus in Flight

    NASA Technical Reports Server (NTRS)

    1991-01-01

    The Perseus proof-of-concept vehicle flies over Rogers Dry Lake at the Dryden Flight Research Center, Edwards, California, to test basic design concepts for the remotely-piloted, high-altitude vehicle. Perseus B is a remotely piloted aircraft developed as a design-performance testbed under NASA's Environmental Research Aircraft and Sensor Technology (ERAST) project. Perseus is one of several flight vehicles involved in the ERAST project. A piston engine, propeller-powered aircraft, Perseus was designed and built by Aurora Flight Sciences Corporation, Manassas, Virginia. The objectives of Perseus B's ERAST flight tests have been to reach and maintain horizontal flight above altitudes of 60,000 feet and demonstrate the capability to fly missions lasting from 8 to 24 hours, depending on payload and altitude requirements. The Perseus B aircraft established an unofficial altitude record for a single-engine, propeller-driven, remotely piloted aircraft on June 27, 1998. It reached an altitude of 60,280 feet. In 1999, several modifications were made to the Perseus aircraft including engine, avionics, and flight-control-system improvements. These improvements were evaluated in a series of operational readiness and test missions at the Dryden Flight Research Center, Edwards, California. Perseus is a high-wing monoplane with a conventional tail design. Its narrow, straight, high-aspect-ratio wing is mounted atop the fuselage. The aircraft is pusher-designed with the propeller mounted in the rear. This design allows for interchangeable scientific-instrument payloads to be placed in the forward fuselage. The design also allows for unobstructed airflow to the sensors and other devices mounted in the payload compartment. The Perseus B that underwent test and development in 1999 was the third generation of the Perseus design, which began with the Perseus Proof-Of-Concept aircraft. Perseus was initially developed as part of NASA's Small High-Altitude Science Aircraft (SHASA

  10. Replication-incompetent adenovirus vector-mediated MDA-7/IL-24 selectively induces growth suppression and apoptosis of hepatoma cell Line SMMC-7721.

    PubMed

    Wang, Congjun; Xue, Xinbo; Yi, Jilin; Wu, Zaide; Chen, Kun; Zheng, Jianwei; Ji, Wenwei; Yu, Yuan

    2008-02-01

    In order to investigate the effect of replication-incompetent adenovirus vector expressing MDA-7/IL-24 on tumor growth and apoptosis of human hepatocellular carcinoma (HCC) cell line SMMC-7721 and normal liver cell line L02, the recombinant replication-incompetent Ad.mda-7 virus vector was constructed and infected into the HCC cell line SMMC-7721 and normal liver cell line L02. RT-PCR was performed to examine the expression of MDA-7 mRNA. The concentrations of MDA-7/IL-4 in culture supernatants were determined by using ELISA. MTT and Hoechst staining assay were applied to observe the inhibitory and killing effects of MDA-7 on the HCC cells. By using flow cytometry, the apoptosis, cell cycle and proliferation of SMMC-7721 and L02 cells were measured. The results showed recombinant replication-incompetent virus expressing MDA-7/IL-24 was constructed successfully, and RT-PCR revealed that it could mediate the high expression of the exogenous gene MDA-7/IL-24 in SMMC-7721 and L02 cells. The expression of MDA-7/IL-24 proteins in the culture supernatant was detectable by ELISA. Ad.mda-7 infection induced apoptosis and growth suppression in SMMC-7721 cells and an increased percentage of HCC cells in the G2/M phase of the cell cycle, but not in L02 cells. It was concluded that mda-7/IL-24 gene, mediated with replication-incompetent adenovirus vector, could selectively induce growth suppression and apoptosis in HCC cell line SMMC-7721 but without any toxic side-effect on normal liver line L02.

  11. Assessment of the State of the Art of Flight Control Technologies as Applicable to Adverse Conditions

    NASA Technical Reports Server (NTRS)

    Reveley, Mary s.; Briggs, Jeffrey L.; Leone, Karen M.; Kurtoglu, Tolga; Withrow, Colleen A.

    2010-01-01

    Literature from academia, industry, and other Government agencies was surveyed to assess the state of the art in current Integrated Resilient Aircraft Control (IRAC) aircraft technologies. Over 100 papers from 25 conferences from the time period 2004 to 2009 were reviewed. An assessment of the general state of the art in adaptive flight control is summarized first, followed by an assessment of the state of the art as applicable to 13 identified adverse conditions. Specific areas addressed in the general assessment include flight control when compensating for damage or reduced performance, retrofit software upgrades to flight controllers, flight control through engine response, and finally test and validation of new adaptive controllers. The state-of-the-art assessment applicable to the adverse conditions include technologies not specifically related to flight control, but may serve as inputs to a future flight control algorithm. This study illustrates existing gaps and opportunities for additional research by the NASA IRAC Project

  12. Regulation of MDA-MB-231 cell proliferation by GSK-3β involves epigenetic modifications under high glucose conditions

    SciTech Connect

    Gupta, Chanchal; Kaur, Jasmine; Tikoo, Kulbhushan

    2014-05-15

    Hyperglycemia is a critical risk factor for development and progression of breast cancer. We have recently reported that high glucose induces phosphorylation of histone H3 at Ser 10 as well as de-phosphorylation of GSK-3β at Ser 9 in MDA-MB-231 cells. Here, we elucidate the mechanism underlying hyperglycemia-induced proliferation in MDA-MB-231 breast cancer cells. We provide evidence that hyperglycemia led to increased DNA methylation and DNMT1 expression in MDA-MB-231 cells. High glucose condition led to significant increase in the expression of PCNA, cyclin D1 and decrease in the expression of PTPN 12, p21 and PTEN. It also induced hypermethylation of DNA at the promoter region of PTPN 12, whereas hypomethylation at Vimentin and Snail. Silencing of GSK-3β by siRNA prevented histone H3 phosphorylation and reduced DNMT1 expression. We show that chromatin obtained after immunoprecipitation with phospho-histone H3 was hypermethylated under high glucose condition, which indicates a cross-talk between DNA methylation and histone H3 phosphorylation. ChIP-qPCR analysis revealed up-regulation of DNMT1 and metastatic genes viz. Vimentin, Snail and MMP-7 by phospho-histone H3, which were down-regulated upon GSK-3β silencing. To the best of our knowledge, this is the first report which shows that interplay between GSK-3β activation, histone H3 phosphorylation and DNA methylation directs proliferation of breast cancer cells. - Highlights: • High glucose induces phosphorylation of histone H3 and dephosphorylation of GSK-3β. • Moreover, hyperglycemia also leads to increased DNA methylation in MDA-MB-231 cells. • Inhibition of GSK-3β prevented histone H3 phosphorylation and reduced DNMT1 levels. • Interplay exists between GSK-3β, histone H3 phosphorylation and DNA methylation.

  13. Elevated serum MDA and depleted non-enzymatic antioxidants, macro-minerals and trace elements are associated with bipolar disorder.

    PubMed

    Chowdhury, Manjurul Islam; Hasan, Maimuna; Islam, Mohammad Safiqul; Sarwar, Md Shahid; Amin, Mohammad Nurul; Uddin, S M Naim; Rahaman, Md Zahedur; Banik, Sujan; Hussain, Md Saddam; Yokota, Kazushige; Hasnat, Abul

    2017-01-01

    Genetic and neurobiological factors are considered to be the major causes of mood and mental disorders. However, over the past few years, increased levels of serum malondialdehyde and altered levels of various non-enzymatic antioxidants and essential minerals involved in abnormal functional activity have been identified as major contributing factors to the pathogenesis of several neurological disorders. The aim of this study was to determine the levels of the serum lipid peroxidation product malondialdehyde (MDA), antioxidants (vitamin A, E and C), macro-minerals (calcium, potassium and sodium) and trace elements (zinc, iron and selenium) in patients with bipolar disorder and to explore their role in disease progression. This is a prospective case-control study that evaluated 55 patients with bipolar disorder and 55 healthy volunteers matched by age and sex. Serum MDA levels were determined by UV spectrophotometry as a marker of lipid peroxidation. RP-HPLC was employed to investigate the serum vitamin A and E concentrations, whereas UV spectrophotometry was used to quantify levels of vitamin C. Serum macro-minerals and trace elements were analyzed by atomic absorption spectroscopy (AAS). Statistical analysis was performed with independent sample t-tests and Pearson's correlation test. We found significantly higher concentrations of MDA (p<0.05) and significantly lower concentrations of antioxidants (vitamin A, E and C) (p<0.05) in the patient group compared with control group. Regarding trace elements and macro-minerals, lower concentrations of zinc, calcium, iron, selenium, sodium and potassium were found in the patient group compared with control subjects (p<0.05). Our study suggests that high serum MDA concentrations and low serum concentrations of antioxidants, macro-minerals and trace elements are strongly associated with bipolar disorder.

  14. 2001 Flight Mechanics Symposium

    NASA Technical Reports Server (NTRS)

    Lynch, John P. (Editor)

    2001-01-01

    This conference publication includes papers and abstracts presented at the Flight Mechanics Symposium held on June 19-21, 2001. Sponsored by the Guidance, Navigation and Control Center of Goddard Space Flight Center, this symposium featured technical papers on a wide range of issues related to attitude/orbit determination, prediction and control; attitude simulation; attitude sensor calibration; theoretical foundation of attitude computation; dynamics model improvements; autonomous navigation; constellation design and formation flying; estimation theory and computational techniques; Earth environment mission analysis and design; and, spacecraft re-entry mission design and operations.

  15. Soaring flight in Guinea

    NASA Technical Reports Server (NTRS)

    Idrac, P

    1920-01-01

    The term soaring is applied here to the flight of certain large birds which maneuver in the air without moving their wings. The author explains the methods of his research and here gives approximate figures for the soaring flight of the Egyptian Vulture and the African White backed Vulture. Figures are given in tabular form for relative air speed per foot per second, air velocity per foot per second, lift/drag ratio, and selected coefficients. The author argues that although the figures given were taken from a very limited series of observations, they have nevertheless thrown some light on the use by birds of the internal energy of the air.

  16. LSRA in flight

    NASA Technical Reports Server (NTRS)

    1993-01-01

    A NASA CV-990, modified as a Landing Systems Research Aircraft (LSRA), in flight over NASA's Dryden Flight Research Center, Edwards, California, for a test of the space shuttle landing gear system. The space shuttle landing gear test unit, operated by a high-pressure hydraulic system, allowed engineers to assess and document the performance of space shuttle main and nose landing gear systems, tires and wheel assemblies, plus braking and nose wheel steering performance. The series of 155 test missions for the space shuttle program provided extensive data about the life and endurance of the shuttle tire systems and helped raise the shuttle crosswind landing limits at Kennedy.

  17. ASTRID rocket flight test

    SciTech Connect

    Whitehead, J.C.; Pittenger, L.C.; Colella, N.J.

    1994-07-01

    On February 4, 1994, we successfully flight tested the ASTRID rocket from Vandenberg Air Force Base. The technology for this rocket originated in the Brilliant Pebbles program and represents a five-year development effort. This rocket demonstrated how our new pumped-propulsion technology-which reduced the total effective engine mass by more than one half and cut the tank mass to one fifth previous requirements-would perform in atmospheric flight. This demonstration paves the way for potential cost-effective uses of the new propulsion system in commercial aerospace vehicles, exploration of the planets, and defense applications.

  18. Hindwings are unnecessary for flight but essential for execution of normal evasive flight in Lepidoptera

    PubMed Central

    Jantzen, Benjamin; Eisner, Thomas

    2008-01-01

    In Lepidoptera, forewings and hindwings are mechanically coupled and flap in synchrony. Flight is anteromotoric, being driven primarily by action of the forewings. Here we report that lepidopterans can still fly when their hindwings are cut off, a procedure reducing their total wing surface, on average, by nearly one half. However, as we demonstrate by analysis of three-dimensional flight trajectories of a moth and a butterfly (Lymantria dispar and Pieris rapae), hindwing removal causes lepidopterans to incur a loss in both linear and turning acceleration, so that they are unable to exercise their normal flight maneuverability. Without hindwings they still are able to zigzag aerially (the ablation has no effect on their turning radius in flight) but at lesser speed and therefore less evasively. Consequently, hindwings in the expanded state in which they occur in lepidopterans seem to contribute in an essential way to lepidopteran survival. Moths in today's world, we argue, may rely on their evasive flight primarily to avoid capture by bats, whereas butterflies, which we propose advertise their evasiveness collectively through shared aposematism, may depend upon it primarily for defense against birds. Aerial agility thus may be the chief adaptive asset derived by lepidopterans from possession of oversize hindwings. PMID:18936482

  19. Flights of Discovery: 50 Years at the NASA Dryden Flight Research Center

    NASA Technical Reports Server (NTRS)

    Wallace, Lance E.

    1996-01-01

    As part of the NASA History Series, this report (NASA SP-4309) describes fifty years of aeronautical research at the NASA Dryden Flight Research Center. Starting with early efforts to exceed the speed of sound with the X-1 aircraft, and continuing through to the X-31 research aircraft, the report covers the flight activities of all of the major research aircraft and lifting bodies studied by NASA. Chapter One, 'A Place for Discovery', describes the facility itself and the surrounding Mojave Desert. Chapter Two, 'The Right Stuff', is about the people involved in the flight research programs. Chapter Three, 'Higher, Faster' summarizes the early years of transonic flight testing and the development of several lifting bodies. Chapter Four, 'Improving Efficiency, Maneuverability & Systems', outlines the development of aeronautical developments such as the supercritical wing, the mission adaptive wing, and various techniques for improving maneuverability fo winged aircraft. Chapter 5, 'Supporting National Efforts', shows how the research activities carried out at Dryden fit into NASA's programs across the country in supporting the space program, in safety and in problem solving related to aircraft design and aviation safety in general. Chapter Six, ' Future Directions' looks to future research building on the fifty year history of aeronautical research at the Dryden Flight Research Center. A glossary of acronyms and an appendix covering concepts and innovations are included. The report also contains many photographs providing a graphical perspective to the historical record.

  20. The Optimization and Characterization of an RNA-Cleaving Fluorogenic DNAzyme Probe for MDA-MB-231 Cell Detection

    PubMed Central

    Xue, Pengcheng; He, Shengnan; Mao, Yu; Qu, Long; Liu, Feng; Tan, Chunyan; Jiang, Yuyang; Tan, Ying

    2017-01-01

    Breast cancer is one of the most frequently diagnosed cancers in females worldwide and lacks specific biomarkers for early detection. In a previous study, we obtained a selective RNA-cleaving Fluorogenic DNAzyme (RFD) probe against MDA-MB-231 cells, typical breast cancer cells, through the systematic evolution of ligands by exponential process (SELEX). To improve the performance of this probe for actual application, we carried out a series of optimization experiments on the pH value of a reaction buffer, the type and concentration of cofactor ions, and sequence minimization. The length of the active domain of the probe reduced to 25 nt from 40 nt after optimization, which was synthesized more easily and economically. The detection limit of the optimized assay system was 2000 MDA-MB-231 cells in 30 min, which is more sensitive than the previous one (almost 5000 cells). The DNAzyme probe was also capable of distinguishing MDA-MB-231 cell specifically from 3 normal cells and 10 other tumor cells. This probe with high sensitivity, selectivity, and economic efficiency enhances the feasibility for further clinical application in breast cancer diagnosis. Herein, we developed an optimization system to produce a general strategy to establish an easy-to-use DNAzyme-based assay for other targets. PMID:28335559

  1. Effects of vitamin-mineral supplementation on cardiac marker and radical scavenging enzymes, and MDA levels in young swimmers.

    PubMed

    Cavas, Levent; Tarhan, Leman

    2004-04-01

    The relationship among the enzyme activities of cardiac markers, the antioxidant defense system, and erythrocyte membrane malonyldialdehyde (MDA) levels related to vitamin-mineral supplementation in swim exercise was investigated. Swimmers aged 11 - 13 years were divided into 2 separate groups as control and vitamin-mineral supplemented. Swimmers participated in a monthly swimming program (4 times/wk) and swam approximately 2- 2.5 km/d. Cardiac markers such as creatine kinase (CK), creatine kinase-MB (CK - MB), glutamic oxaloacetic transaminase [GOT (AST)], lactate dehydrogenase (LDH), and anti-oxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities in post-training samples were found to be significantly (p<.05) higher than in pre-training samples. Except for GOT (AST), the activity increases in CK, CK-MB, and LDH in female and male supplemented groups were significantly (p<.05) lower than those of control groups during the 1-month period of swim training. Antioxidant enzyme activity increases in the male vitamin-mineral group were significantly (p <.05) higher when compared with the other groups. Post-training MDA levels were significantly (p <.001) higher than pre-training MDA levels in the control groups, whereas no significant (p<.05) differences were found between the vitamin-mineral supplemented groups. Vitamin-mineral supplementation was found to attenuate cardiac and muscle damage markers while also enhancing antioxidant levels and reducing membrane LPO levels in response to 1 month of swim training.

  2. Mitotic arrest of breast cancer MDA-MB-231 cells by a halogenated thieno[3,2-d]pyrimidine.

    PubMed

    Ross, Christina R; Temburnikar, Kartik W; Wilson, Gerald M; Seley-Radtke, Katherine L

    2015-04-15

    Halogenated thieno[3,2-d]pyrimidines exhibit antiproliferative activity against a variety of cancer cell models, such as the mouse lymphocytic leukemia cell line L1210 in which they induce apoptosis independent of cell cycle arrest. Here we assessed these activities on MDA-MB-231 cells, a well-established model of aggressive, metastatic breast cancer. While 2,4-dichloro[3,2-d]pyrimidine was less toxic to MDA-MB-231 cells than previously observed in the L1210 model, flow cytometry analysis showed that MDA-MB-231 cell death involved arrest at the G2/M stage of the cell cycle. Conversely, the introduction of bromine at C7 of the 2,4-dichloro[3,2-d]pyrimidine eliminated cell type-dependent differences in cytotoxicity or cell cycle status. Together, these data indicate that a substituent at C7 can profoundly modify the cytotoxic mechanism of halogenated thieno[3,2-d]pyrimidines in a cell type-specific manner.

  3. Identification of a Bacillus thuringiensis Surface-Layer-Protein with Cytotoxic Activity against MDA-MB-231 Breast Cancer Cells.

    PubMed

    Rubio, Viviana P; Bravo, Alejandra; Olmos, Jorge

    2016-10-06

    In this work we isolated a Surface-Layer-Protein (SLP) from a Bacillus thuringiensis (Bt) strain to evaluate it cytotoxic effects against MDA-MB-231 human breast cancer cells. AP11 was selected from a group of Bt strains using SLP oligonucleotides developed from Bacillus conserved regions. AP11 strain was grown in Luria Bertani (LB) medium until late exponential phase; an 86 kDa protein was extracted using 5 M LiCl and identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). It corresponded to a multispecies S-layer protein highly similar to previously described SLP in B. thuringiensis. MDA-MB-231 breast cancer cells LC₅₀ was obtained using 0.25 µg/ml of the isolated SLP. HaCat non-cancerous cells presented 90% survival using the same protein concentration. Our data suggest that SLP cytotoxicity against MDA-MB-231 could be induced by an interaction with CDH11 cell membrane receptor.

  4. Differential Epigenetic Effects of Atmospheric Cold Plasma on MCF-7 and MDA-MB-231 Breast Cancer Cells

    PubMed Central

    Park, Sung-Bin; Kim, Byungtak; Bae, Hansol; Lee, Hyunkyung; Lee, Seungyeon; Choi, Eun H.; Kim, Sun Jung

    2015-01-01

    Cold atmospheric plasma (plasma) has emerged as a novel tool for a cancer treatment option, having been successfully applied to a few types of cancer cells, as well as tissues. However, to date, no studies have been performed to examine the effect of plasma on epigenetic alterations, including CpG methylation. In this study, the effects of plasma on DNA methylation changes in breast cancer cells were examined by treating cultured MCF-7 and MDA-MB-231 cells, representing estrogen-positive and estrogen-negative cancer cells, respectively, with plasma. A pyrosequencing analysis of Alu indicated that a specific CpG site was induced to be hypomethylated from 23.4 to 20.3% (p < 0.05) by plasma treatment in the estrogen-negative MDA-MB-231 cells only. A genome-wide methylation analysis identified “cellular movement, connective tissue development and function, tissue development” and “cell-to-cell signaling and interaction, cell death and survival, cellular development” as the top networks. Of the two cell types, the MDA-MB-231 cells underwent a higher rate of apoptosis and a decreased proliferation rate upon plasma treatment. Taken together, these results indicate that plasma induces epigenetic and cellular changes in a cell type-specific manner, suggesting that a careful screening of target cells and tissues is necessary for the potential application of plasma as a cancer treatment option. PMID:26042423

  5. Summary of Research on Reliability Criteria-Based Flight System Control

    NASA Technical Reports Server (NTRS)

    Wu, N. Eva; Belcastro, Christine (Technical Monitor)

    2002-01-01

    This paper presents research on the reliability assessment of adaptive flight control systems. The topics include: 1) Overview of Project Focuses; 2) Reliability Analysis; and 3) Design for Reliability. This paper is presented in viewgraph form.

  6. Space Shuttle flight control system

    NASA Technical Reports Server (NTRS)

    Klinar, W. J.; Kubiak, E. T.; Peters, W. H.; Saldana, R. L.; Smith, E. E., Jr.; Stegall, H. W.

    1975-01-01

    The Space Shuttle is a control stabilized vehicle with control provided by an all digital, fly-by-wire flight control system. This paper gives a description of the several modes of flight control which correspond to the Shuttle mission phases. These modes are ascent flight control (including open loop first stage steering, the use of four computers operating in parallel and inertial guidance sensors), on-orbit flight control (with a discussion of reaction control, phase plane switching logic, jet selection logic, state estimator logic and OMS thrust vector control), entry flight control and TAEM (terminal area energy management to landing). Also discussed are redundancy management and backup flight control.

  7. Effects of an 18-day flight on the human body.

    PubMed

    Yegorov, A D; Kakurin, L I; Nefyodov, Y G

    1972-01-01

    During their flight on board the Soyuz 9 A.G. Nikolayev and V.I. Sevastyanov adapted to weightlessness by the 3rd-4th day and stabilized their physiological functions by the end of the mission. During flight both cosmonauts maintained normal performance, sleep and appetite; they readily developed a new stereotype of movements and exhibited no noticeable increase of the circulatory function in response to a standard physical load. In contrast to the effects of shorter term flights, this mission "used unusual and distressing feelings in the crew members aggravated by distinct changes in the major- physiological systems during the first day of recovery. In the immediate postflight hours the transition from the recumbent to the sitting position brought about circulation disorders; 24 hours later the cosmonauts still walked with uncertainty and kept the erect position at rest on account of a significant elevation of their centre of gravity. Weight losses, shifts of water and mineral metabolism, bone tissue demineralization and symptoms of orthostatic intolerance observed in this flight were similar to those resulting from earlier short-term missions. Of importance was a dysbacteriotic change in the skin and nasal microflora. Physiological changes in the Soyuz 9 crew members were functional and reversible, being on the whole in agreement with predicted effects. These results call for the development of specific measures facilitating the post-flight adaptation of space pilots in view of future long duration space flights.

  8. Innovative use of global navigation satellite systems for flight inspection

    NASA Astrophysics Data System (ADS)

    Kim, Eui-Ho

    Inspection Systems (FIS) by using GPS and WAAS in novel manners. The algorithms include Adaptive Carrier Smoothing (ACS), optimizing WAAS accuracy and stability, and reference point-based precise relative positioning for real-time and near-real-time applications. The developed systems are WAAS-aided FIS, WAAS-based FIS, and stand-alone GPS-based FIS. These systems offer both high efficiency and low cost, and they have different advantages over one another in terms of accuracy, integrity, and worldwide availability. The performance of each system is tested with experimental flight test data and shown to have accuracy that is sufficient for flight inspection and superior to the current Inertial-based AFIS.

  9. Neurovestibular considerations for sub-orbital space flight: A framework for future investigation.

    PubMed

    Karmali, Faisal; Shelhamer, Mark

    2010-01-01

    Commercial sub-orbital operators will soon offer the excitement of traveling to space to thousands of people. Based on previous experience in space flight and parabolic flight, sensorimotor disruptions in eye movements, postural stability, and motor coordination are likely in these travelers. Here we propose a framework for developing strategies to overcome these sensorimotor disruptions. We delineate how approaches should differ from those applied to orbital flight and between sub-orbital passengers and pilots based on differing frequency of flights and mission objectives. Sensorimotor adaptation is one strategy for overcoming disruptions; an important question is whether it occurs quickly enough to be of use during periods of reduced and enhanced gravity lasting less than five minutes. Data are presented showing that sensorimotor adaptation of the pitch vestibulo-ocular reflex during parabolic flight takes a few consecutive days of flying to overcome an initial disruption. We conclude with recommendations for operators and researchers to improve safety and comfort during sub-orbital operations. We recommend using parabolic flight as a tool for pre-adapting sub-orbital passengers, along with further research into the required quantity and timing of these pre-adaptation flights and the tasks conducted during these flights. Likewise, for sub-orbital pilots, we recommend emphasizing recency of experience.

  10. Neurovestibular Considerations for Sub-Orbital Space Flight: A Framework for Future Investigation

    PubMed Central

    Karmali, Faisal; Shelhamer, Mark

    2013-01-01

    Commercial sub-orbital operators will soon offer the excitement of traveling to space to thousands of people. Based on previous experience in space flight and parabolic flight, sensorimotor disruptions in eye movements, postural stability, and motor coordination are likely in these travelers. Here we propose a framework for developing strategies to overcome these sensorimotor disruptions. We delineate how approaches should differ from those applied to orbital flight and between sub-orbital passengers and pilots based on differing frequency of flights and mission objectives. Sensorimotor adaptation is one strategy for overcoming disruptions; an important question is whether it occurs quickly enough to be of use during periods of reduced and enhanced gravity lasting less than five minutes. Data are presented showing that sensorimotor adaptation of the pitch vestibulo-ocular reflex during parabolic flight takes a few consecutive days of flying to overcome an initial disruption. We conclude with recommendations for operators and researchers to improve safety and comfort during sub-orbital operations. We recommend using parabolic flight as a tool for pre-adapting sub-orbital passengers, along with further research into the required quantity and timing of these pre-adaptation flights and the tasks conducted during these flights. Likewise, for sub-orbital pilots, we recommend emphasizing recency of experience. PMID:20555165

  11. A 2.5 mW/ch, 50 Mcps, 10-Analog Channel, Adaptively Biased Read-Out Front-End IC With Low Intrinsic Timing Resolution for Single-Photon Time-of-Flight PET Applications With Time-Dependent Noise Analysis in 90 nm CMOS.

    PubMed

    Cruz, Hugo; Huang, Hong-Yi; Luo, Ching-Hsing; Lee, Shuenn-Yuh

    2017-04-01

    This paper presents a 10-channel time-of-flight application-specific integrated circuit (ASIC) for positron emission tomography in a 90 nm standard CMOS process. To overcome variations in channel-to-channel timing resolution caused by mismatch and process variations, adaptive biases and a digital-to-analog converter (DAC) are utilized. The main contributions of this work are as follows. First, multistage architectures reduce the total power consumption, and detection bandwidths of analog preamplifiers and comparators are increased to 1 and 1.5 GHz, respectively, relative to those in previous studies. Second, a total intrinsic electronic timing resolution of 9.71 ps root-mean-square (RMS) is achieved (13.88 ps peak and 11.8 ps average of the 10 channels in 5 ASICs). Third, the proposed architecture reduces variations in channel-to-channel timing resolution to 2.6 bits (equivalent to 4.17 ps RMS) by calibrating analog comparator threshold levels. A 181.5 ps full-width-at-half-maximum timing resolution is measured with an avalanche photo diode and a laser setup. The power consumption is 2.5 mW using 0.5 and 1.2 V power supplies. The proposed ASIC is implemented in a 90 nm TSMC CMOS process with a total area of 3.3 mm × 2.7 mm.

  12. Insect Flight and MAVs

    DTIC Science & Technology

    2007-11-02

    flight u After 350 million years of evolution , they have probably found good solutions for – Kinematics – Wing design – Control Systems High-Lift...Plane Episyrphus balteatus Hoverflies, dragonflies , small birds and bats rely on dynamic stall on the downstroke (red) for weight support Fling

  13. Weather and Flight Testing

    NASA Technical Reports Server (NTRS)

    Wiley, Scott

    2007-01-01

    This viewgraph document reviews some of the weather hazards involved with flight testing. Some of the hazards reviewed are: turbulence, icing, thunderstorms and winds and windshear. Maps, pictures, satellite pictures of the meteorological phenomena and graphs are included. Also included are pictures of damaged aircraft.

  14. Flight Termination Criteria

    NASA Astrophysics Data System (ADS)

    Haber, Jerold M.; Larson, Erik

    2013-09-01

    The first line of defense in protecting the public against the threat of injury from a failing space booster is the flight termination system. Consequently, these systems must be highly reliable and the criteria for flight termination must be carefully formulated. Criteria must be developed based on observable data that allows adequate time for the data to be assessed and a flight termination action to be triggered. Criteria should be set so that 1) the chance a good vehicle will be terminated is small, 2) the chance of failing to terminate an errant vehicle before it can hazard population centers or valuable assets is minimal, and 3) there is assurance that the combination of the planned trajectory and mission rules do not induce excessive risks to land based populations, air lanes, and shipping lanes should the vehicle need to be terminated [1].This paper provides an overview of the approaches to developing and implement flight termination criteria and a tool for understanding risk implications of proposed criteria.

  15. White Flight: Some Hypotheses.

    ERIC Educational Resources Information Center

    Wegmann, Robert G.

    The available literature on white flight, or, more properly, school resegregation -- the phenomenon of white withdrawal (total or partial) from desegregated schools -- is reviewed in this paper which also reports some new research in this area. The distinction is made between those schools located on the fringes of the inner city, which first…

  16. Autonomous Formation Flight

    NASA Technical Reports Server (NTRS)

    Schkolnik, Gerard S.; Cobleigh, Brent

    2004-01-01

    NASA's Strategic Plan for the Aerospace Technology Enterprise includes ambitious objectives focused on affordable air travel, reduced emissions, and expanded aviation-system capacity. NASA Dryden Flight Research Center, in cooperation with NASA Ames Research Center, the Boeing Company, and the University of California, Los Angeles, has embarked on an autonomous-formation-flight project that promises to make significant strides towards these goals. For millions of years, birds have taken advantage of the aerodynamic benefit of flying in formation. The traditional "V" formation flown by many species of birds (including gulls, pelicans, and geese) enables each of the trailing birds to fly in the upwash flow field that exists just outboard of the bird immediately ahead in the formation. The result for each trailing bird is a decrease in induced drag and thus a reduction in the energy needed to maintain a given speed. Hence, for migratory birds, formation flight extends the range of the system of birds over the range of birds flying solo. The Autonomous Formation Flight (AFF) Project is seeking to extend this symbiotic relationship to aircraft.

  17. Flight Model Discharge System

    DTIC Science & Technology

    1989-09-01

    Itterconnection wiring diagram for the ESA ............................... 34 3-13 Typical gain versus total count curve for CEM...42 3-16 Calibration curve for energy bin 12 of the ion ESA ....................... 43 3-17 Flight ESA S/N001...Calibration curves for SPM S/N001 ......................................... 67 4-11 Calibration curves for SPM S/N002

  18. F-104 in flight

    NASA Technical Reports Server (NTRS)

    1993-01-01

    F-104G N826NA during a 1993 flight over the Mojave desert, outfitted with an experiment pylon under the center fuselage and wing racks. The F-104 was originally designed by Kelly Johnson of the Lockheed Skunk Works as a day fighter. The aircraft soon proved ideal for both research and training. For instance, a modified F-104 tested the reaction control jets for the X-15. The F-104's short wings and low lift to drag ratio made it ideal to simulate the X-15 landing profile, which the F-104s often undertook before X-15 flights in order to acquaint pilots with the rocket plane's landing characteristics. This training role continued with the lifting bodies. NASA F-104s were also used for high-speed research after the X-1E was retired. Finally, the F-104s were also used as chase planes for research missions. The F-104G was a late model designed as a fighter bomber for low-level strike missions. It was built for use by the West German Air Force and other foreign governments. N826NA accomplished a wide-range of research activities, including tests of the Space Shuttle's Thermal Protection System (TPS) tiles. The aircraft made 1,415 flights before being retired. It is now on display at the Dryden Flight Research Center.

  19. Flight Mechanics Symposium 1997

    NASA Technical Reports Server (NTRS)

    Walls, Donna M. (Editor)

    1997-01-01

    This conference publication includes papers and abstracts presented at the Flight Mechanics Symposium. This symposium featured technical papers on a wide range of issues related to orbit-attitude prediction, determination, and control; attitude sensor calibration; attitude determination error analysis; attitude dynamics; and orbit decay and maneuver strategy. Government, industry, and the academic community participated in the preparation and presentation of these papers.

  20. Flight telerobotic servicer legacy

    NASA Astrophysics Data System (ADS)

    Shattuck, Paul L.; Lowrie, James W.

    1992-11-01

    The Flight Telerobotic Servicer (FTS) was developed to enhance and provide a safe alternative to human presence in space. The first step for this system was a precursor development test flight (DTF-1) on the Space Shuttle. DTF-1 was to be a pathfinder for manned flight safety of robotic systems. The broad objectives of this mission were three-fold: flight validation of telerobotic manipulator (design, control algorithms, man/machine interfaces, safety); demonstration of dexterous manipulator capabilities on specific building block tasks; and correlation of manipulator performance in space with ground predictions. The DTF-1 system is comprised of a payload bay element (7-DOF manipulator with controllers, end-of-arm gripper and camera, telerobot body with head cameras and electronics module, task panel, and MPESS truss) and an aft flight deck element (force-reflecting hand controller, crew restraint, command and display panel and monitors). The approach used to develop the DTF-1 hardware, software and operations involved flight qualification of components from commercial, military, space, and R controller, end-of-arm tooling, force/torque transducer) and the development of the telerobotic system for space applications. The system is capable of teleoperation and autonomous control (advances state of the art); reliable (two-fault tolerance); and safe (man-rated). Benefits from the development flight included space validation of critical telerobotic technologies and resolution of significant safety issues relating to telerobotic operations in the Shuttle bay or in the vicinity of other space assets. This paper discusses the lessons learned and technology evolution that stemmed from developing and integrating a dexterous robot into a manned system, the Space Shuttle. Particular emphasis is placed on the safety and reliability requirements for a man-rated system as these are the critical factors which drive the overall system architecture. Other topics focused on include

  1. Workshop on Exercise Prescription for Long-Duration Space Flight

    NASA Technical Reports Server (NTRS)

    Harris, Bernard A., Jr. (Editor); Stewart, Donald F. (Editor)

    1989-01-01

    The National Aeronautics and Space Administration has a dedicated history of ensuring human safety and productivity in flight. Working and living in space long term represents the challenge of the future. Our concern is in determining the effects on the human body of living in space. Space flight provides a powerful stimulus for adaptation, such as cardiovascular and musculoskeletal deconditioning. Extended-duration space flight will influence a great many systems in the human body. We must understand the process by which this adaptation occurs. The NASA is agressively involved in developing programs which will act as a foundation for this new field of space medicine. The hallmark of these programs deals with prevention of deconditioning, currently referred to as countermeasures to zero g. Exercise appears to be most effective in preventing the cardiovascular and musculoskeletal degradation of microgravity.

  2. Spacewedge #1 in Flight

    NASA Technical Reports Server (NTRS)

    1992-01-01

    A Spacewedge subscale model, built to help develop potential autonomous recovery systems for spacecraft as well as methods for delivering large Army cargo loads to precision landings, maneuvers through the air under its steerable parafoil during 1992 flight testing. From October 1991 to December 1996, NASA Ames-Dryden Flight Research Facility (after 1994, the Dryden Flight Research Center, Edwards, California) conducted a research program know as the Spacecraft Autoland Project. This Project was designed to determine the feasibility of the autonomous recovery of a spacecraft using a ram-air parafoil system for the final stages of flight, including a precision landing. The Johnson Space Center and the U.S. Army participated in various phases of the program. The Charles Stark Draper Laboratory developed the software for Wedge 3 under contract to the Army. Four generic spacecraft (each called a Spacewedge or simply a Wedge) were built; the last one was built to test the feasibility of a parafoil for delivering Army cargoes. Technology developed during this program has applications for future spacecraft recovery systems, such as the X-38 Crew Return Vehicle demonstrator. The Spacewedge program demonstrated precision flare and landing into the wind at a predetermined location. The program showed that a flexible, deployable system using autonomous navigation and landing was a viable and practical way to recover spacecraft. NASA researchers conducted flight tests of the Spacewedge at three sites near Dryden, a hillside near Tehachapi, the Rogers Dry Lakebed at Edwards Air Force Base, and the California City Airport Drop Zone. During the first phase of testing 36 flights were made. Phase II consisted of 45 flights using a smaller parafoil. A third Phase of 34 flights was conducted primarily by the Army and resulted in the development of an Army guidance system for precision offset cargo delivery. The wedge used during the Army phase was not called a Spacewedge but simply a

  3. Examination of Epigenetic and other Molecular Factors Associated with mda-9/Syntenin Dysregulation in Cancer Through Integrated Analyses of Public Genomic Datasets

    PubMed Central

    Bacolod, Manny D.; Das, Swadesh K.; Sokhi, Upneet K.; Bradley, Steven; Fenstermacher, David A.; Pellecchia, Maurizio; Emdad, Luni; Sarkar, Devanand; Fisher, Paul B.

    2016-01-01

    mda-9/Syntenin (melanoma differentiation-associated gene 9) is a PDZ domain-containing, cancer invasion-related protein. In this study, we employed multiple integrated bioinformatic approaches to identify the probable epigenetic factors, molecular pathways, and functionalities associated with mda-9 dysregulation during cancer progression. Analyses of publicly available genomic data (e.g., expression, copy number, methylation) from TCGA, GEO, ENCODE, and Human Protein Atlas projects led to the following observations: a) mda-9 expression correlates with both copy number and methylation level of an intronic CpG site (cg17197774) located downstream of the CpG island, b) cg17197774 methylation is a likely prognostic marker in glioma, c) Among 22 cancer types, melanoma exhibits the highest mda-9 level, and lowest level of methylation at cg17197774, d) cg17197774 hypomehtylation is also associated with histone modifications (at the mda-9 locus) indicative of more active transcription, e) Using Gene Set Enrichment Analysis (GSEA), and the VIGOR (Virtual Gene Over-expression or Repression ) analytical scheme, we were able to predict mda-9’s association with extracellular matrix organization (e.g., MMPs, collagen, integrins), IGFBP2 and NF-κB signaling pathways, phospholipid metabolism, cytokines (e.g., interleukins), CTLA-4, and components of complement cascade pathways. Indeed, previous publications have shown that many of the aforementioned genes and pathways are associated with mda-9’s functionality. PMID:26093898

  4. Matrix metalloproteinases are involved in both type I (apoptosis) and type II (autophagy) cell death induced by sodium phenylacetate in MDA-MB-231 breast tumour cells.

    PubMed

    Augustin, Sébastien; Berard, Madeleine; Kellaf, Sabine; Peyri, Nicole; Fauvel-Lafève, Françoise; Legrand, Chantal; He, Lu; Crépin, Michel

    2009-04-01

    The effects of sodium phenylacetate (NaPa), an antitumoral molecule, on cell death and matrix metalloproteinase (MMP) activities and synthesis were investigated in two metastatic breast tumour cell lines, MDA-MB-231 and MDA-MB-435, cultured on three-dimensional type I collagen gels (3-D cultures). In both cell lines, NaPa inhibited cell proliferation and induced apoptotic cell death as measured by TUNEL assay, with an IC(30) of 20 mM and 10 mM for MDA-MB-231 and MDA-MB-435 cells, respectively. In MDA-MB-231 cells, NaPa also induced (i) an autophagic process evidenced by the appearance of autophagic vacuoles and an increased phosphatase acid activity, (ii) the formation of pseudopodia and (iii) an increase in MMP-1 and MMP-9 secretion without affecting MT1-MMP. In NaPa-treated MDA-MB-435 cells, no autophagic vacuoles were formed but F-actin depolymerisation was observed. MMP-1, MMP-9 and MT1-MMP levels were strongly enhanced in these cells but MMPs were not secreted and accumulated intracellularly. When breast cancer cells were treated with NaPa in the presence of an MMP inhibitor (GM6001), apoptotic cell death decreased and the induction of autophagic vacuoles in MDA-MB-231 cells was inhibited. Taken together, these data suggest that MMPs are involved in the autophagic cell death and/or apoptosis of breast tumour cells.

  5. Pathfinder aircraft flight

    NASA Technical Reports Server (NTRS)

    1996-01-01

    The Pathfinder research aircraft's wing structure is clearly defined as it soars under a clear blue sky during a test flight from Dryden Flight Research Center, Edwards, California, in November of 1996. Pathfinder was a lightweight, solar-powered, remotely piloted flying wing aircraft used to demonstrate the use of solar power for long-duration, high-altitude flight. Its name denotes its mission as the 'Pathfinder' or first in a series of solar-powered aircraft that will be able to remain airborne for weeks or months on scientific sampling and imaging missions. Solar arrays covered most of the upper wing surface of the Pathfinder aircraft. These arrays provided up to 8,000 watts of power at high noon on a clear summer day. That power fed the aircraft's six electric motors as well as its avionics, communications, and other electrical systems. Pathfinder also had a backup battery system that could provide power for two to five hours, allowing for limited-duration flight after dark. Pathfinder flew at airspeeds of only 15 to 20 mph. Pitch control was maintained by using tiny elevators on the trailing edge of the wing while turns and yaw control were accomplished by slowing down or speeding up the motors on the outboard sections of the wing. On September 11, 1995, Pathfinder set a new altitude record for solar-powered aircraft of 50,567 feet above Edwards Air Force Base, California, on a 12-hour flight. On July 7, 1997, it set another, unofficial record of 71,500 feet at the Pacific Missile Range Facility, Kauai, Hawaii. In 1998, Pathfinder was modified into the longer-winged Pathfinder Plus configuration. (See the Pathfinder Plus photos and project description.)

  6. A Comprehensive Analysis of the X-15 Flight 3-65 Accident

    NASA Technical Reports Server (NTRS)

    Dennehy, Cornelius J.; Orr, Jeb S.; Barshi, Immanuel; Statler, Irving C.

    2014-01-01

    The November 15, 1967, loss of X-15 Flight 3-65-97 (hereafter referred to as Flight 3-65) was a unique incident in that it was the first and only aerospace flight accident involving loss of crew on a vehicle with an adaptive flight control system (AFCS). In addition, Flight 3-65 remains the only incidence of a single-pilot departure from controlled flight of a manned entry vehicle in a hypersonic flight regime. To mitigate risk to emerging aerospace systems, the NASA Engineering and Safety Center (NESC) proposed a comprehensive review of this accident. The goal of the assessment was to resolve lingering questions regarding the failure modes of the aircraft systems (including the AFCS) and thoroughly analyze the interactions among the human agents and autonomous systems that contributed to the loss of the pilot and aircraft. This document contains the outcome of the accident review.

  7. Free flight odor tracking in Drosophila: Effect of wing chemosensors, sex and pheromonal gene regulation

    PubMed Central

    Houot, Benjamin; Gigot, Vincent; Robichon, Alain; Ferveur, Jean-François

    2017-01-01

    The evolution of powered flight in insects had major consequences for global biodiversity and involved the acquisition of adaptive processes allowing individuals to disperse to new ecological niches. Flies use both vision and olfactory input from their antennae to guide their flight; chemosensors on fly wings have been described, but their function remains mysterious. We studied Drosophila flight in a wind tunnel. By genetically manipulating wing chemosensors, we show that these structures play an essential role in flight performance with a sex-specific effect. Pheromonal systems are also involved in Drosophila flight guidance: transgenic expression of the pheromone production and detection gene, desat1, produced low, rapid flight that was absent in control flies. Our study suggests that the sex-specific modulation of free-flight odor tracking depends on gene expression in various fly tissues including wings and pheromonal-related tissues. PMID:28067325

  8. META II Complexity and Adaptability

    DTIC Science & Technology

    2011-08-01

    looks for the highest peaks, Robust Design looks for the highest mesas – regions where the performance does not fall off due to small variations in...ATA 23 (Communications), ATA 24(Electrical Power), ATA 27(Flight Controls), ATA 29(Hydraulics), ATA 30(Ice and Rain Protection ),ATA 31(Indicating...though a degree of adaptation may be required. 4.5. Experimentation The experiments we have performed in the program fall into two broad categories

  9. The NASA Flight Demonstration Program

    NASA Technical Reports Server (NTRS)

    Levin, G. M.; Routh, D.

    1985-01-01

    The Flight Demonstration Program was initiated by the Office of Space Flight in October 1983. The purposes of this program are to conduct an ongoing series of flight demonstrations which demonstrate critical enabling technology to potential Shuttle users and at the same time provide hands on experience to NASA engineers. Flight demonstrations are selected through a peer review process in response to an annual solicitation issued by the Flight Demonstration Office at NASA Headquarters. As of July 1985 one major flight demonstration has been successfully conducted, two flight demonstrations are scheduled to fly in November on STS 61-B, and five flight demonstrations are in various stages of development and planned to be flown during the next three years.

  10. Boeing flight deck design philosophy

    NASA Technical Reports Server (NTRS)

    Stoll, Harty

    1990-01-01

    Information relative to Boeing flight deck design philosophy is given in viewgraph form. Flight deck design rules, design considerations, functions allocated to the crew, redundancy and automation concerns, and examples of accident data that were reviewed are listed.

  11. Flight stability analysis under changes in insect morphology

    NASA Astrophysics Data System (ADS)

    Noest, Robert; Wang, Z. Jane

    2015-11-01

    Insect have an amazing ability to control their flight, being able to perform both fast aerial maneuvers and stable hovering. The insect's neural system has developed various mechanism by which it can control these flying feats, but we expect that insect morphology is equally important in facilitating the aerial control. We perform a computational study using a quasi-steady instantaneous flapping flight model which allows us to freely adapt the insect's morphological parameters. We picked a fruit fly as the basis for the body shape and wing motion, and study the effect of changes to the morphology for a range of wing stroke amplitudes. In each case we determine the periodic flight mode, with the period equal to a single wing beat, and do a Floquet stability analysis of the flight. To interpret our results we will compare the changed morphology to related insects. We discuss the implications of the insects location on the stability diagram.

  12. On-Line Safe Flight Envelope Determination for Impaired Aircraft

    NASA Technical Reports Server (NTRS)

    Lombaerts, Thomas; Schuet, Stefan; Acosta, Diana; Kaneshige, John

    2015-01-01

    The design and simulation of an on-line algorithm which estimates the safe maneuvering envelope of aircraft is discussed in this paper. The trim envelope is estimated using probabilistic methods and efficient high-fidelity model based computations of attainable equilibrium sets. From this trim envelope, a robust reachability analysis provides the maneuverability limitations of the aircraft through an optimal control formulation. Both envelope limits are presented to the flight crew on the primary flight display. In the results section, scenarios are considered where this adaptive algorithm is capable of computing online changes to the maneuvering envelope due to impairment. Furthermore, corresponding updates to display features on the primary flight display are provided to potentially inform the flight crew of safety critical envelope alterations caused by the impairment.

  13. Spacelab 1 and the Life Sciences Flight Experiments Program

    NASA Technical Reports Server (NTRS)

    Bush, W. H.; Clark, R. S.

    1984-01-01

    The Life Sciences Flight Experiments Program (LSFEP) was established by NASA in 1978 to plan and direct efforts necessary to conduct a continuing program of in-flight life science investigations throughout the Space Shuttle era. The Spacelab 1 (SL-1) mission, conducted from November 28 to December 8, 1983, was to verify Spacelab performance through a variety of scientific experiments including life science. A description is given of the seven NASA life sciences experiments, which consisted of four human experiments, a fungus experiment, a plant experiment, and radiation experiments. Ten life sciences experiments from the European Space Agency were also flown. The experiments include studies of the circadian rhythms in Neurospora crassa, the nutation of Helianthus annus, the vestibular function during weightlessness, the influence of space flight on erythrokinetics in man, and the adaptation of vestibulo-spinal reflex mechanisms during space flight.

  14. Sprague-Dawley rats display sex-linked differences in the pharmacokinetics of 3,4-methylenedioxymethamphetamine (MDMA) and its metabolite 3,4-methylenedioxyamphetamine (MDA)

    SciTech Connect

    Fonsart, Julien; Menet, Marie-Claude; Debray, Marcel; Hirt, Deborah; Noble, Florence; Scherrmann, Jean-Michel; Decleves, Xavier

    2009-12-15

    The use of 3,4-methylenedioxymethamphetamine (MDMA, ecstasy) has increased in recent years; it can lead to life-threatening hyperthermia and serotonin syndrome. Human and rodent males appear to be more sensitive to acute toxicity than are females. MDMA is metabolized to five main metabolites by the enzymes CYP1A2, CYP2D and COMT. Little is presently known about sex-dependent differences in the pharmacokinetics of MDMA and its metabolites. We therefore analyzed MDMA disposition in male and female rats by measuring the plasma and urine concentrations of MDMA and its metabolites using a validated LC-MS method. MDA AUC{sub last} and C{sub max} were 1.6- to 1.7-fold higher in males than in females given MDMA (5 mg/kg sc), while HMMA C{sub max} and AUC{sub last} were 3.2- and 3.5-fold higher, respectively. MDMA renal clearance was 1.26-fold higher in males, and that of MDA was 2.2-fold higher. MDMA AUC{sub last} and t{sub 1/2} were 50% higher in females given MDMA (1 mg/kg iv). MDA C{sub max} and AUC{sub last} were 75-82% higher in males, with a 2.8-fold higher metabolic index. Finally, the AUC{sub last} of MDA was 0.73-fold lower in males given 1 mg/kg iv MDA. The volumes of distribution of MDMA and MDA at steady-state were similar in the two sexes. These data strongly suggest that differences in the N-demethylation of MDMA to MDA are major influences on the MDMA and MDA pharmacokinetics in male and female rats. Hence, males are exposed to significantly more toxic MDA, which could explain previously reported sexual dysmorphism in the acute effects and toxicity of MDMA in rats.

  15. Activated human mesenchymal stem/stromal cells suppress metastatic features of MDA-MB-231 cells by secreting IFN-β.

    PubMed

    Yoon, N; Park, M S; Shigemoto, T; Peltier, G; Lee, R H

    2016-04-14

    Our recent study showed that human mesenchymal stem/stromal cells (hMSCs) are activated to express tumor necrosis factor (TNF)-α-related apoptosis-inducing ligand (TRAIL) by exposure to TNF-α and these activated hMSCs effectively induce apoptosis in triple-negative breast cancer MDA-MB-231 (MDA) cells in vitro and in vivo. Here, we further demonstrated that activated hMSCs not only induced apoptosis of MDA cells but also reduced metastatic features in MDA cells. These activated hMSC-exposed MDA cells showed reduced tumorigenicity and suppressed formation of lung metastasis when implanted in the mammary fat pad. Surprisingly, the activated hMSC-exposed MDA cells increased TRAIL expression, resulting in apoptosis in MDA cells. Interestingly, upregulation of TRAIL in MDA cells was mediated by interferon-beta (IFN-β) secreted from activated hMSCs. Furthermore, IFN-β in activated hMSCs was induced by RNA and DNA released from apoptotic MDA cells in absent in melanoma 2 (AIM2) and IFN induced with helicase C domain 1 (IFIH1)-dependent manners. These observations were only seen in the TRAIL-sensitive breast cancer cell lines but not in the TRAIL-resistant breast cancer cell lines. Consistent with these results, Kaplan-Meier survival analysis also showed that lack of innate sensors detecting DNA or RNA is strongly associated with poor survival in estrogen receptor-negative breast cancer patients. In addition, cancer-associated fibroblasts (CAF) isolated from a breast cancer patient were also able to express TRAIL and IFN-β upon DNA and RNA stimulation. Therefore, our results suggest that the crosstalk between TRAIL-sensitive cancer cells and stromal cells creates a tumor-suppressive microenvironment and further provide a novel therapeutic approach to target stromal cells within cancer microenvironment for TRAIL sensitive cancer treatment.

  16. Early SP-100 flight mission designs

    NASA Astrophysics Data System (ADS)

    Josloff, Allan T.; Shepard, Neal F.; Kirpich, Aaron S.; Murata, Ronald; Smith, Michael A.; Stephen, James D.

    1993-01-01

    Early flight mission objectives can be met with a Space Reactor Power System (SRPS) using thermoelectric conversion in conjunction with fast spectrum, lithium-cooled reactors. This paper describes two system design options using thermoelectric technology to accommodate an early launch. In the first of these options, radiatively coupled Radioiosotope Thermoelectric Generator (RTG) unicouples are adapted for use with a SP-100-type reactor heat source. Unicouples have been widely used as the conversion technology in RTGs and have demonstrated the long-life characteristics necessary for a highly relible SRPS. The thermoelectric leg height is optimized in conjunction with the heat rejection temperature to provide a mass optimum 6-kWe system configured for launch on a Delta II launch vehicle. The flight-demonstrated status of this conversion technology provides a high confidence that such a system can be designed, assembled, tested, and launched by 1997. The use of a SP-100-type reactor assures compliance with safety requirements and expedites the flight safety approval process while, at the same time, providing flight performance verification for a heat source technology with the growth potential to meet future national needs for higher power levels. A 15-kW2, Atlas IIAS-launched system using the compact, conductively coupled multicouple converters being developed under the SP-100 program to support an early flight system launch also described. Both design concepts have been scaled to 20-kWe in order to support recent studies by DOE/NASA for higher power early launch missions.

  17. [Correction of autonomic reactions parameters in organism of cosmonaut with adaptive biocontrol method].

    PubMed

    Kornilova, L N; Cowings, P S; Toscano, W B; Arlashchenko, N I; Korneev, D Iu; Ponomarenko, A V; Salagovich, S V; Sarantseva, A V; Kozlovskaia, I B

    2000-01-01

    Presented are results of testing the method of adaptive biocontrol during preflight training of cosmonauts. Within the MIR-25 crew, a high level of controllability of the autonomous reactions was characteristic of Flight Commanders MIR-23 and MIR-25 and flight Engineer MIR-23, while Flight Engineer MIR-25 displayed a weak intricate dependence of these reactions on the depth of relaxation or strain.

  18. [Correction of autonomic reactions parameters in organism of cosmonaut with adaptive biocontrol method

    NASA Technical Reports Server (NTRS)

    Kornilova, L. N.; Cowings, P. S.; Toscano, W. B.; Arlashchenko, N. I.; Korneev, D. Iu; Ponomarenko, A. V.; Salagovich, S. V.; Sarantseva, A. V.; Kozlovskaia, I. B.

    2000-01-01

    Presented are results of testing the method of adaptive biocontrol during preflight training of cosmonauts. Within the MIR-25 crew, a high level of controllability of the autonomous reactions was characteristic of Flight Commanders MIR-23 and MIR-25 and flight Engineer MIR-23, while Flight Engineer MIR-25 displayed a weak intricate dependence of these reactions on the depth of relaxation or strain.

  19. Adaptive SPECT

    PubMed Central

    Barrett, Harrison H.; Furenlid, Lars R.; Freed, Melanie; Hesterman, Jacob Y.; Kupinski, Matthew A.; Clarkson, Eric; Whitaker, Meredith K.

    2008-01-01

    Adaptive imaging systems alter their data-acquisition configuration or protocol in response to the image information received. An adaptive pinhole single-photon emission computed tomography (SPECT) system might acquire an initial scout image to obtain preliminary information about the radiotracer distribution and then adjust the configuration or sizes of the pinholes, the magnifications, or the projection angles in order to improve performance. This paper briefly describes two small-animal SPECT systems that allow this flexibility and then presents a framework for evaluating adaptive systems in general, and adaptive SPECT systems in particular. The evaluation is in terms of the performance of linear observers on detection or estimation tasks. Expressions are derived for the ideal linear (Hotelling) observer and the ideal linear (Wiener) estimator with adaptive imaging. Detailed expressions for the performance figures of merit are given, and possible adaptation rules are discussed. PMID:18541485

  20. F/A-18D (RC)/ATARS flight test update

    NASA Astrophysics Data System (ADS)

    White, P. S.; Gallo, Lori A. M.; Borland, Robert; De Groh, William B.; Minor, John L.

    1995-09-01

    In June 1994, the U.S. Navy and U.S. Marine Corps restructured the F/A-18D (RC) ATARS program. McDonnell Douglas Aerospace was selected as the prime contractor responsible for overall program management and integration of the ATARS with the F/A-18D (RC) weapon system. Loral Fairchild Systems (LFS) was selected as the subcontractor responsible for ATARS subsystem development and integration. The first part of the restructured program was called Element One and was a ten month risk reduction program ending in April 1995. The primary objective of Element One was to resolve the critical ATARS anomalies found during previous flight testing. In order to resolve these anomalies, MDA and LFS concurred that additional flight test data was required. In November/December 1994, eight flights were flown with a high fidelity instrumentation system to gather the additional data required for evaluation of the anomalies. Using this data, along with previously collected data, design 'fixes' were implemented to correct several of the system anomalies. In April 1995 and May 1995, approximately four flight tests were conducted to evaluate the performance of these design 'fixes.' A period of two months for flight tests was planned for July-August 1995 to complete this evaluation. This presentation describes the testing and results through May 1995 of the Element One flight test program.

  1. Cardiovascular physiology in space flight

    NASA Technical Reports Server (NTRS)

    Charles, John B.; Bungo, Michael W.

    1991-01-01

    The effects of space flight on the cardiovascular system have been studied since the first manned flights. In several instances, the results from these investigations have directly contradicted the predictions based on established models. Results suggest associations between space flight's effects on other organ systems and those on the cardiovascular system. Such findings provide new insights into normal human physiology. They must also be considered when planning for the safety and efficiency of space flight crewmembers.

  2. Space Flight Operations Center local area network

    NASA Technical Reports Server (NTRS)

    Goodman, Ross V.

    1988-01-01

    The existing Mission Control and Computer Center at JPL will be replaced by the Space Flight Operations Center (SFOC). One part of the SFOC is the LAN-based distribution system. The purpose of the LAN is to distribute the processed data among the various elements of the SFOC. The SFOC LAN will provide a robust subsystem that will support the Magellan launch configuration and future project adaptation. Its capabilities include (1) a proven cable medium as the backbone for the entire network; (2) hardware components that are reliable, varied, and follow OSI standards; (3) accurate and detailed documentation for fault isolation and future expansion; and (4) proven monitoring and maintenance tools.

  3. Automation Framework for Flight Dynamics Products Generation

    NASA Technical Reports Server (NTRS)

    Wiegand, Robert E.; Esposito, Timothy C.; Watson, John S.; Jun, Linda; Shoan, Wendy; Matusow, Carla

    2010-01-01

    XFDS provides an easily adaptable automation platform. To date it has been used to support flight dynamics operations. It coordinates the execution of other applications such as Satellite TookKit, FreeFlyer, MATLAB, and Perl code. It provides a mechanism for passing messages among a collection of XFDS processes, and allows sending and receiving of GMSEC messages. A unified and consistent graphical user interface (GUI) is used for the various tools. Its automation configuration is stored in text files, and can be edited either directly or using the GUI.

  4. YF-12 in flight

    NASA Technical Reports Server (NTRS)

    1972-01-01

    The Flight Research Center's involvement with the YF-12A, an interceptor version of the Lockheed A-12, began in 1967. Ames Research Center was interested in using wind tunnel data that had been generated at Ames under extreme secrecy. Also, the Office of Advanced Research and Technology (OART) saw the YF-12A as a means to advance high-speed technology, which would help in designing the Supersonic Transport (SST). The Air Force needed technical assistance to get the latest reconnaissance version of the A-12 family, the SR-71A, fully operational. Eventually, the Air Force offered NASA the use of two YF-12A aircraft, 60-6935 and 60-6936. A joint NASA-USAF program was mapped out in June 1969. NASA and Air Force technicians spent three months readying 935 for flight. On 11 December 1969, the flight program got underway with a successful maiden flight piloted by Col. Joe Rogers and Maj. Gary Heidelbaugh of the SR-71/F-12 Test Force. During the program, the Air Force concentrated on military applications, and NASA pursued a loads research program. NASA studies included inflight heating, skin-friction cooling, 'coldwall' research (a heat transfer experiment), flowfield studies, shaker vane research, and tests in support of the Space Shuttle landing program. Ultimately, 935 became the workhorse of the program, with 146 flights between 11 December 1969 and 7 November 1979. The second YF-12A, 936, made 62 flights. It was lost in a non-fatal crash on 24 June 1971. It was replaced by the so-called YF-12C (SR-71A 61-7951, modified with YF-12A inlets and engines and a bogus tail number 06937). The Lockheed A-12 family, known as the Blackbirds, were designed by Clarence 'Kelly' Johnson. They were constructed mostly of titanium to withstand aerodynamic heating. Fueled by JP-7, the Blackbirds were capable of cruising at Mach 3.2 and attaining altitudes in excess of 80,000 feet. The first version, a CIA reconnaissance aircraft that first flew in April 1962 was called the A-12. An

  5. Design of a digital adaptive control system for reentry vehicles.

    NASA Technical Reports Server (NTRS)

    Picon-Jimenez, J. L.; Montgomery, R. C.; Grigsby, L. L.

    1972-01-01

    The flying qualities of atmospheric reentry vehicles experience considerable variations due to the wide changes in flight conditions characteristic of reentry trajectories. A digital adaptive control system has been designed to modify the vehicle's dynamic characteristics and to provide desired flying qualities for all flight conditions. This adaptive control system consists of a finite-memory identifier which determines the vehicle's unknown parameters, and a gain computer which calculates feedback gains to satisfy flying quality requirements.

  6. NASA Dryden Flight Research Center

    NASA Technical Reports Server (NTRS)

    Navarro, Robert

    2009-01-01

    This DVD has several short videos showing some of the work that Dryden is involved in with experimental aircraft. These are: shots showing the Active AeroElastic Wing (AAW) loads calibration tests, AAW roll maneuvers, AAW flight control surface inputs, Helios flight, and takeoff, and Pathfinder takeoff, flight and landing.

  7. Flight crew health stabilization program

    NASA Technical Reports Server (NTRS)

    Wooley, B. C.; Mccollum, G. W.

    1975-01-01

    The flight crew health stabilization program was developed to minimize or eliminate the possibility of adverse alterations in the health of flight crews during immediate preflight, flight, and postflight periods. The elements of the program, which include clinical medicine, immunology, exposure prevention, and epidemiological surveillance, are discussed briefly. No crewmember illness was reported for the missions for which the program was in effect.

  8. Bisphosphonate ISS Flight Experiment

    NASA Technical Reports Server (NTRS)

    LeBlanc, Adrian; Matsumoto, Toshio; Jones, Jeffrey; Shapiro, Jay; Lang, Thomas; Shackleford, Linda; Smith, Scott M.; Evans, Harlan; Spector, Elizabeth; Ploutz-Snyder, Robert; Sibonga, Jean; Keyak, Joyce; Nakamura, Toshitaka; Kohri, Kenjiro; Ohshima, Hiroshi; Moralez, Gilbert

    2014-01-01

    The bisphosphonate study is a collaborative effort between the NASA and JAXA space agencies to investigate the potential for antiresorptive drugs to mitigate bone changes associated with long-duration spaceflight. Elevated bone resorption is a hallmark of human spaceflight and bed rest (common zero-G analog). We tested whether an antiresorptive drug in combination with in-flight exercise would ameliorate bone loss and hypercalcuria during longduration spaceflight. Measurements include DXA, QCT, pQCT, and urine and blood biomarkers. We have completed analysis of 7 crewmembers treated with alendronate during flight and the immediate postflight (R+<2 week) data collection in 5 of 10 controls without treatment. Both groups used the advanced resistive exercise device (ARED) during their missions. We previously reported the pre/postflight results of crew taking alendronate during flight (Osteoporosis Int. 24:2105-2114, 2013). The purpose of this report is to present the 12-month follow-up data in the treated astronauts and to compare these results with preliminary data from untreated crewmembers exercising with ARED (ARED control) or without ARED (Pre-ARED control). Results: the table presents DXA and QCT BMD expressed as percentage change from preflight in the control astronauts (18 Pre-ARED and the current 5 ARED-1-year data not yet available) and the 7 treated subjects. As shown previously the combination of exercise plus antiresorptive is effective in preventing bone loss during flight. Bone measures for treated subjects, 1 year after return from space remain at or near baseline values. Except in one region, the treated group maintained or gained bone 1 year after flight. Biomarker data are not currently available for either control group and therefore not presented. However, data from other studies with or without ARED show elevated bone resorption and urinary Ca excretion while bisphosphonate treated subjects show decreases during flight. Comparing the two control

  9. 14 CFR 121.414 - Initial and transition training and checking requirements: flight instructors (airplane), flight...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ....414 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED... training for flight instructors (airplane), flight engineer instructors (airplane), and flight navigator... instruction. (4) For flight engineer instructors (airplane) and flight navigator instructors (airplane),...

  10. 14 CFR 91.109 - Flight instruction; Simulated instrument flight and certain flight tests.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... certain flight tests. (a) No person may operate a civil aircraft (except a manned free balloon) that is... used for a flight test for an airline transport pilot certificate or a class or type rating on...

  11. Vestibular adaptation to space in monkeys

    NASA Technical Reports Server (NTRS)

    Dai, M.; Raphan, T.; Kozlovskaya, I.; Cohen, B.

    1998-01-01

    Otolith-induced eye movements of rhesus monkeys were studied before and after the 1989 COSMOS 2044 and the 1992 to 1993 COSMOS 2229 flights. Two animals flew in each mission for approximately 2 weeks. After flight, spatial orientation of the angular vestibulo-ocular reflex was altered. In one animal the time constant of postrotatory nystagmus, which had been shortened by head tilts with regard to gravity before flight, was unaffected by the same head tilts after flight. In another animal, eye velocity, which tended to align with a gravitational axis before flight, moved toward a body axis after flight. This shift of orientation disappeared by 7 days after landing. After flight, the magnitude of compensatory ocular counter-rolling was reduced by about 70% in both dynamic and static tilts. Modulation in vergence in response to naso-occipital linear acceleration during off-vertical axis rotation was reduced by more than 50%. These changes persisted for 11 days after recovery. An up and down asymmetry of vertical nystagmus was diminished for 7 days. Gains of the semicircular canal-induced horizontal and vertical angular vestibulo-ocular reflexes were unaffected in both flights, but the gain of the roll angular vestibulo-ocular reflex was decreased. These data indicate that there are short- and long-term changes in otolith-induced eye movements after adaptation to microgravity. These experiments also demonstrate the unique value of the monkey as a model for studying effects of vestibular adaptation in space. Eye movements can be measured in three dimensions in response to controlled vestibular and visual stimulation, and the results are directly applicable to human beings. Studies in monkeys to determine how otolith afferent input and central processing is altered by adaptation to microgravity should be an essential component of future space-related research.

  12. Becoming a flight surgeon.

    PubMed

    Gallé-Tessonneau, J R

    1988-12-01

    This text is the inaugural lesson given by the Professor of Aeronautic Psychiatry and starts the training period for new flight surgeons in the French Air Force. Introducing the French Air Force Medicine Training Session, the author speaks about the psychological aspects in aviation medicine. Three points of pilots' psychology are developed: 1) the pilot's body as the source of intense sensations and as an object of important value; 2) the libidinal, narcissistic, and defensive aspects of the pilot's spirit; and 3) the pilot's environment with its characteristic relationships. These facts influence the medical approach and modify the physician-pilot relationship. The flight surgeon must pay attention and get ready for this specific practice.

  13. Optimal symmetric flight studies

    NASA Technical Reports Server (NTRS)

    Weston, A. R.; Menon, P. K. A.; Bilimoria, K. D.; Cliff, E. M.; Kelley, H. J.

    1985-01-01

    Several topics in optimal symmetric flight of airbreathing vehicles are examined. In one study, an approximation scheme designed for onboard real-time energy management of climb-dash is developed and calculations for a high-performance aircraft presented. In another, a vehicle model intermediate in complexity between energy and point-mass models is explored and some quirks in optimal flight characteristics peculiar to the model uncovered. In yet another study, energy-modelling procedures are re-examined with a view to stretching the range of validity of zeroth-order approximation by special choice of state variables. In a final study, time-fuel tradeoffs in cruise-dash are examined for the consequences of nonconvexities appearing in the classical steady cruise-dash model. Two appendices provide retrospective looks at two early publications on energy modelling and related optimal control theory.

  14. Effects of space flight on locomotor control

    NASA Technical Reports Server (NTRS)

    Bloomberg, Jacob J.; Layne, Charles S.; McDonald, P. Vernon; Peters, Brian T.; Huebner, William P.; Reschke, Millard F.; Berthoz, Alain; Glasauer, Stefan; Newman, Dava; Jackson, D. Keoki

    1999-01-01

    In the microgravity environment of spaceflight, the relationship between sensory input and motor output is altered. During prolonged missions, neural adaptive processes come into play to recalibrate central nervous system function, thereby permitting new motor control strategies to emerge in the novel sensory environment of microgravity. However, the adaptive state achieved during spaceflight is inappropriate for a unit gravity environment and leads to motor control alterations upon return to Earth that include disturbances in locomotion. Indeed, gait and postural instabilities following the return to Earth have been reported in both U.S. astronauts and Russian cosmonauts even after short duration (5- to 10-day) flights. After spaceflight, astronauts may: (1) experience the sensation of turning while attempting to walk a straight path, (2) encounter sudden loss of postural stability, especially when rounding corners, (3) perceive exaggerated pitch and rolling head movements during walking, (4) experience sudden loss of orientation in unstructured visual environments, or (5) experience significant oscillopsia during locomotion.

  15. Climate adaptation

    NASA Astrophysics Data System (ADS)

    Kinzig, Ann P.

    2015-03-01

    This paper is intended as a brief introduction to climate adaptation in a conference devoted otherwise to the physics of sustainable energy. Whereas mitigation involves measures to reduce the probability of a potential event, such as climate change, adaptation refers to actions that lessen the impact of climate change. Mitigation and adaptation differ in other ways as well. Adaptation does not necessarily have to be implemented immediately to be effective; it only needs to be in place before the threat arrives. Also, adaptation does not necessarily require global, coordinated action; many effective adaptation actions can be local. Some urban communities, because of land-use change and the urban heat-island effect, currently face changes similar to some expected under climate change, such as changes in water availability, heat-related morbidity, or changes in disease patterns. Concern over those impacts might motivate the implementation of measures that would also help in climate adaptation, despite skepticism among some policy makers about anthropogenic global warming. Studies of ancient civilizations in the southwestern US lends some insight into factors that may or may not be important to successful adaptation.

  16. Flight Day 2 Highlights

    NASA Technical Reports Server (NTRS)

    2003-01-01

    The STS-107 second flight day begins with a shot of the Spacehab Research Double Module. Live presentations of experiments underway inside of the Spacehab Module are presented. Six experiments are shown. As part of the Space Technology and Research Student Payload, students from Australia, China, Israel, Japan, New York, and Liechtenstein are studying the effect that microgravity has on ants, spiders, silkworms, fish, bees, granular materials, and crystals. Mission Specialist Kalpana Chawla is seen working with the zeolite crystal growth experiment.

  17. MARS Flight Engineering Status

    SciTech Connect

    Fast, James E.; Dorow, Kevin E.; Morris, Scott J.; Thompson, Robert C.; Willett, Jesse A.

    2010-04-06

    The Multi-sensor Airborne Radiation Survey Flight Engineering project (MARS FE) has designed a high purity germanium (HPGe) crystal array for conducting a wide range of field measurements. In addition to the HPGe detector system, a platform-specific shock and vibration isolation system and environmental housing have been designed to support demonstration activities in a maritime environment on an Unmanned Surface Vehicle (USV). This report describes the status of the equipment as of the end of FY09.

  18. Infrared Thermography Flight Experimentation

    NASA Technical Reports Server (NTRS)

    Blanchard, Robert C.; Carter, Matthew L.; Kirsch, Michael

    2003-01-01

    Analysis was done on IR data collected by DFRC on May 8, 2002. This includes the generation of a movie to initially examine the IR flight data. The production of the movie was challenged by the volume of data that needed to be processed, namely 40,500 images with each image (256 x 252) containing over 264 million points (pixel depth 4096). It was also observed during the initial analysis that the RTD surface coating has a different emissivity than the surroundings. This fact added unexpected complexity in obtaining a correlation between RTD data and IR data. A scheme was devised to generate IR data near the RTD location which is not affected by the surface coating This scheme is valid as long as the surface temperature as measured does not change too much over a few pixel distances from the RTD location. After obtaining IR data near the RTD location, it is possible to make a direct comparison with the temperature as measured during the flight after adjusting for the camera s auto scaling. The IR data seems to correlate well to the flight temperature data at three of the four RID locations. The maximum count intensity occurs closely to the maximum temperature as measured during flight. At one location (RTD #3), there is poor correlation and this must be investigated before any further progress is possible. However, with successful comparisons at three locations, it seems there is great potential to be able to find a calibration curve for the data. Moreover, as such it will be possible to measure temperature directly from the IR data in the near future.

  19. Flight Crew Health Maintenance

    NASA Technical Reports Server (NTRS)

    Gullett, C. C.

    1970-01-01

    The health maintenance program for commercial flight crew personnel includes diet, weight control, and exercise to prevent heart disease development and disability grounding. The very high correlation between hypertension and overweight in cardiovascular diseases significantly influences the prognosis for a coronary prone individual and results in a high rejection rate of active military pilots applying for civilian jobs. In addition to physical fitness the major items stressed in pilot selection are: emotional maturity, glucose tolerance, and family health history.

  20. Dryden Flight Research Center

    NASA Technical Reports Server (NTRS)

    Kostyk, Christopher Barry

    2007-01-01

    As part of a session at the 2007 Thermal & Fluids Analysis Workshop (TFAWS), an overview of the operations at NASA Dryden Flight Research Center was given. Mission support at this site includes the Aeronautics Research Mission Directorate (ARMD); Exploration Systems Mission Directorate (ESMD), Science - ER-2; Science - G3 UAVSAR; Science - Ikhana and Space Operations. In addition, the presentation describes TFAWS related work at Dryden.

  1. Flight Software Math Library

    NASA Technical Reports Server (NTRS)

    McComas, David

    2013-01-01

    The flight software (FSW) math library is a collection of reusable math components that provides typical math utilities required by spacecraft flight software. These utilities are intended to increase flight software quality reusability and maintainability by providing a set of consistent, well-documented, and tested math utilities. This library only has dependencies on ANSI C, so it is easily ported. Prior to this library, each mission typically created its own math utilities using ideas/code from previous missions. Part of the reason for this is that math libraries can be written with different strategies in areas like error handling, parameters orders, naming conventions, etc. Changing the utilities for each mission introduces risks and costs. The obvious risks and costs are that the utilities must be coded and revalidated. The hidden risks and costs arise in miscommunication between engineers. These utilities must be understood by both the flight software engineers and other subsystem engineers (primarily guidance navigation and control). The FSW math library is part of a larger goal to produce a library of reusable Guidance Navigation and Control (GN&C) FSW components. A GN&C FSW library cannot be created unless a standardized math basis is created. This library solves the standardization problem by defining a common feature set and establishing policies for the library s design. This allows the libraries to be maintained with the same strategy used in its initial development, which supports a library of reusable GN&C FSW components. The FSW math library is written for an embedded software environment in C. This places restrictions on the language features that can be used by the library. Another advantage of the FSW math library is that it can be used in the FSW as well as other environments like the GN&C analyst s simulators. This helps communication between the teams because they can use the same utilities with the same feature set and syntax.

  2. X-29 flight maneuvers

    NASA Technical Reports Server (NTRS)

    1989-01-01

    Two X-29 aircraft, featuring one of the most unusual designs in aviation history, were flown at the NASA Dryden Flight Research Center, Edwards, Calif., as technology demonstrators to investigate a host of advanced concepts and technologies. This 30-second clip of air-to-air footage shows the X-29 as it makes hard left and right aileron turns followed up with a few barrel rolls.

  3. Advanced flight control system study

    NASA Technical Reports Server (NTRS)

    Hartmann, G. L.; Wall, J. E., Jr.; Rang, E. R.; Lee, H. P.; Schulte, R. W.; Ng, W. K.

    1982-01-01

    A fly by wire flight control system architecture designed for high reliability includes spare sensor and computer elements to permit safe dispatch with failed elements, thereby reducing unscheduled maintenance. A methodology capable of demonstrating that the architecture does achieve the predicted performance characteristics consists of a hierarchy of activities ranging from analytical calculations of system reliability and formal methods of software verification to iron bird testing followed by flight evaluation. Interfacing this architecture to the Lockheed S-3A aircraft for flight test is discussed. This testbed vehicle can be expanded to support flight experiments in advanced aerodynamics, electromechanical actuators, secondary power systems, flight management, new displays, and air traffic control concepts.

  4. Flight Project Data Book

    NASA Technical Reports Server (NTRS)

    1992-01-01

    The Office of Space Science and Applications (OSSA) is responsible for the overall planning, directing, executing, and evaluating that part of the overall NASA program that has the goal of using the unique characteristics of the space environment to conduct a scientific study of the universe, to understand how the Earth works as an integrated system, to solve practical problems on Earth, and to provide the scientific and technological research foundation for expanding human presence beyond Earth orbit into the solar system. OSSA guides its program toward leadership through its pursuit of excellence across the full spectrum of disciplines. OSSA pursues these goals through an integrated program of ground-based laboratory research and experimentation, suborbital flight of instruments on airplanes, balloons, and sounding rockets; flight of instruments and the conduct of research on the Shuttle/Spacelab system and on Space Station Freedom; and development and flight of automated Earth-orbiting and interplanetary spacecraft. The OSSA program is conducted with the participation and support of other Government agencies and facilities, universities throughout the United States, the aerospace contractor community, and all of NASA's nine Centers. In addition, OSSA operates with substantial international participation in many aspects of our Space Science and Applications Program. OSSA's programs currently in operation, those approved for development, and those planned for future missions are described.

  5. Solar array flight experiment

    NASA Technical Reports Server (NTRS)

    1986-01-01

    Emerging satellite designs require increasing amounts of electrical power to operate spacecraft instruments and to provide environments suitable for human habitation. In the past, electrical power was generated by covering rigid honeycomb panels with solar cells. This technology results in unacceptable weight and volume penalties when large amounts of power are required. To fill the need for large-area, lightweight solar arrays, a fabrication technique in which solar cells are attached to a copper printed circuit laminated to a plastic sheet was developed. The result is a flexible solar array with one-tenth the stowed volume and one-third the weight of comparably sized rigid arrays. An automated welding process developed to attack the cells to the printed circuit guarantees repeatable welds that are more tolerant of severe environments than conventional soldered connections. To demonstrate the flight readiness of this technology, the Solar Array Flight Experiment (SAFE) was developed and flown on the space shuttle Discovery in September 1984. The tests showed the modes and frequencies of the array to be very close to preflight predictions. Structural damping, however, was higher than anticipated. Electrical performance of the active solar panel was also tested. The flight performance and postflight data evaluation are described.

  6. Advanced glycation endproducts increase proliferation, migration and invasion of the breast cancer cell line MDA-MB-231.

    PubMed

    Sharaf, Hana; Matou-Nasri, Sabine; Wang, Qiuyu; Rabhan, Zaki; Al-Eidi, Hamad; Al Abdulrahman, Abdulkareem; Ahmed, Nessar

    2015-03-01

    Diabetic patients have increased likelihood of developing breast cancer. Advanced glycation endproducts (AGEs) underlie the pathogenesis of diabetic complications but their impact on breast cancer cells is not understood. This study aims to determine the effects of methylglyoxal-derived bovine serum albumin AGEs (MG-BSA-AGEs) on the invasive MDA-MB-231 breast cancer cell line. By performing cell counting, using wound-healing assay, invasion assay and zymography analysis, we found that MG-BSA-AGEs increased MDA-MB-231 cell proliferation, migration and invasion through Matrigel™ associated with an enhancement of matrix metalloproteinase (MMP)-9 activities, in a dose-dependent manner. Using Western blot and flow cytometry analyses, we demonstrated that MG-BSA-AGEs increased expression of the receptor for AGEs (RAGE) and phosphorylation of key signaling protein extracellular signal-regulated kinase (ERK)-1/2. Furthermore, in MG-BSA-AGE-treated cells, phospho-protein micro-array analysis revealed enhancement of phosphorylation of the ribosomal protein 70 serine S6 kinase beta 1 (p70S6K1), which is known to be involved in protein synthesis, the signal transducer and activator of transcription (STAT)-3 and the mitogen-activated protein kinase (MAPK) p38, which are involved in cell survival. Blockade of MG-BSA-AGE/RAGE interactions using a neutralizing anti-RAGE antibody inhibited MG-BSA-AGE-induced MDA-MB-231 cell processes, including the activation of signaling pathways. Throughout the study, non-modified BSA had a negligible effect. In conclusion, AGEs might contribute to breast cancer development and progression partially through the regulation of MMP-9 activity and RAGE signal activation. The up-regulation of RAGE and the concomitant increased phosphorylation of p70S6K1 induced by AGEs may represent promising targets for drug therapy to treat diabetic patients with breast cancer.

  7. High throughput screening of natural products for anti-mitotic effects in MDA-MB-231 human breast carcinoma cells

    PubMed Central

    Mazzio, E; Badisa, R; Mack, N; Deiab, S; Soliman, KFA

    2013-01-01

    Some of the most effective anti-mitotic microtubule-binding agents, such as paclitaxel (Taxus brevifolia) were originally discovered through robust NCI botanical screenings. In this study, a high-through microarray format was utilized to screen 897 aqueous extracts of commonly used natural products (0.00015–0.5 mg/ml) relative to paclitaxel for anti-mitotic effects (independent of toxicity) on proliferation of MDA-MB-231 cells. The data obtained showed that less than 1.34 % tested showed inhibitory growth (IG50) properties <0.0183 mg/ml. The most potent anti-mitotics (independent of toxicity) were Mandrake root (Podophyllum peltatum), Truja Twigs (Thuja occidentalis), Colorado desert mistletoe (Phoradendron flavescens), Tou Gu Cao Speranskia Herb (Speranskia tuberculata), Bentonite Clay, Bunge Root (Pulsatilla chinensis), Brucea Fruit (Brucea javanica), Madder Root (Rubia tinctorum), Gallnut of Chinese Sumac (Melaphis chinensis), Elecampane Root (Inula Helenium), Yuan Zhi Root (Polygala tenuifolia), Pagoda Tree Fruit (Melia Toosendan), Stone Root (Collinsonia Canadensis) and others such as American Witchhazel, Arjun and Bladderwrack. The strongest tumoricidal herbs identified from amongst the subset evaluated for anti-mitotic properties were wild yam (Dioscorea villosa), beth-root (Trillium Pendulum) and alkanet-root (Lithospermum canescens). Additional data was obtained on a lesser-recognized herb: (Speranskia tuberculata) which showed growth inhibition on BT-474 (human ductal breast carcinoma) and Ishikawa (human endometrial adenocarcinoma) cells with ability to block replicative DNA synthesis leading to G2 arrest in MDA-MB-231 cells. In conclusion, these findings present relative potency of natural anti-mitotic resources effective against human breast carcinoma MDA-MB-231 cell division. PMID:24105850

  8. Use of Virtual Reality for Space Flight

    NASA Technical Reports Server (NTRS)

    Harm, Deborah; Taylor, L. C.; Reschke, M. F.

    2011-01-01

    Virtual environments offer unique training opportunities, particularly for training astronauts and preadapting them to the novel sensory conditions of microgravity. Two unresolved human factors issues in virtual reality (VR) systems are: 1) potential "cybersickness", and 2) maladaptive sensorimotor performance following exposure to VR systems. Interestingly, these aftereffects are often quite similar to adaptive sensorimotor responses observed in astronauts during and/or following space flight. Active exploratory behavior in a new environment, with resulting feedback and the formation of new associations between sensory inputs and response outputs, promotes appropriate perception and motor control in the new environment. Thus, people adapt to consistent, sustained alterations of sensory input such as those produced by microgravity. Our research examining the effects of repeated exposures to a full field of view dome VR system showed that motion sickness and initial decrements in eye movement and postural control were greatly diminished following three exposures. These results suggest that repeated transitions between VR and the normal environment preflight might be a useful countermeasure for neurosensory and sensorimotor effects of space flight. The range of VR applications is enormous, extending from ground-based VR training for extravehicular activities at NASA, to medical and educational uses. It seems reasonable to suggest that other space related uses of VR should be investigated. For example, 1) use of head-mounted VR on orbit to rehearse/practice upcoming operational activities, and 2) ground-based VR training for emergency egress procedures. We propose that by combining VR designed for operational activities preflight, along with an appropriate schedule to facilitate sensorimotor adaptation and improve spatial orientation would potentially accomplish two important goals for astronauts and cosmonauts, preflight sensorimotor adaption and enhanced operational

  9. Evaluation of 3M molecular detection assay (MDA) Salmonella for the detection of Salmonella in selected foods: collaborative study.

    PubMed

    Bird, Patrick; Fisher, Kiel; Boyle, Megan; Huffman, Travis; Benzinger, M Joseph; Bedinghaus, Paige; Flannery, Jonathan; Crowley, Erin; Agin, James; Goins, David; Benesh, DeAnn; David, John

    2013-01-01

    The 3M Molecular Detection Assay (MDA) Salmonella is used with the 3M Molecular Detection System for the detection of Salmonella spp. in food, food-related, and environmental samples after enrichment. The assay utilizes loop-mediated isothermal amplification to rapidly amplify Salmonella target DNA with high specificity and sensitivity, combined with bioluminescence to detect the amplification. The 3M MDA Salmonella method was compared using an unpaired study design in a multilaboratory collaborative study to the U.S. Department of Agriculture/Food Safety and Inspection Service-Microbiology Laboratory Guidebook (USDA/FSIS-MLG 4.05), Isolation and Identification of Salmonella from Meat, Poultry, Pasteurized Egg and Catfish Products for raw ground beef and the U.S. Food and Drug Administration/Bacteriological Analytical Manual (FDA/BAM) Chapter 5 Salmonella reference method for wet dog food following the current AOAC guidelines. A total of 20 laboratories participated. For the 3M MDA Salmonella method, raw ground beef was analyzed using 25 g test portions, and wet dog food was analyzed using 375 g test portions. For the reference methods, 25 g test portions of each matrix were analyzed. Each matrix was artificially contaminated with Salmonella at three inoculation levels: an uninoculated control level (0 CFU/test portion), a low inoculum level (0.2-2 CFU/test portion), and a high inoculum level (2-5 CFU/test portion). In this study, 1512 unpaired replicate samples were analyzed. Statistical analysis was conducted according to the probability of detection (POD). For the low-level raw ground beef test portions, the following dLPOD (difference between the POD of the reference and candidate method) values with 95% confidence intervals were obtained: -0.01 (-0.14, +0.12). For the low-level wet dog food test portions, the following dLPOD with 95% confidence intervals were obtained: -0.04 (-0.16, +0.09). No significant differences were observed in the number of positive

  10. Disialyl GD2 ganglioside suppresses ICAM-1-mediated invasiveness in human breast cancer MDA-MB231 cells

    PubMed Central

    Kwon, Kyung-Min; Chung, Tae-Wook; Kwak, Choong-Hwan; Choi, Hee-Jung; Kim, Kyung-Woon; Ha, Sun-Hyung; Cho, Seung-Hak; Lee, Young-Choon; Ha, Ki-Tae; Lee, Moon-Jo; Kim, Cheorl-Ho

    2017-01-01

    The disialoganglioside GD3 has been considered to be involved in tumor progression or suppression in various tumor cells. However, the significance of the biological functions of GD3 in breast cancer cells is still controversial. This prompted us to study the possible relationship(s) between GD3 expression and the metastatic potential of a breast cancer MDA-MB231 cells as an estrogen receptor negative (ER-) type. The human GD3 synthase cDNA was transfected into MDA-MB231 cells, and G-418 bulk selection was used to select cells stably overexpressing the GD3 synthase. In vitro invasion potentials of the GD3 synthase over-expressing cells (pc3-GD3s) were significantly suppressed when compared with control cells. Expression of intercellular adhesion molecule-1 (ICAM-1; CD54) was down-regulated in the pc3-GD3s cells and the decrease in ICAM-I expression is directly related to the decrease in invasiveness of the pc3-GD3s cells. Another type of ER negative SK-BR3 cells exhibited the similar level of ICAM-1 expression as MDA-MB231 cells, while the ER positive MCF-7 cells (ER+) showed the increased expression level of ICAM-1. Then, we investigated signaling pathways known to control ICAM-1 expression. No difference was observed in the phosphorylation of ERK and p38 between the pc3-GD3s and control cells (pc3), but the activation of AKT was inhibited in pc3-GD3s, and not in the control (pc3). In addition, the composition of total gangliosides was changed between control (pc3) and pc3-GD3s cells, as confirmed by HPTLC. The pc3-GD3s cells had an accumulation of the GD2 instead of the GD3. RT-PCR results showed that not only GD3 synthase, but also GM2/GD2 synthase (β4-GalNc T) expression was increased in pc3-GD3s cells. Overexpression of GD3 synthase suppresses the invasive potential of human breast cancer MDA-MB-231 cells through down-regulation of ICAM-1 and the crucial pathway to allow the apoptotic effect has been attributed to accumulation of the GD2 ganglioside. ER has

  11. Dexterous Manipulation in Microgravity in Parabolic Flights and on ISS

    NASA Astrophysics Data System (ADS)

    Pletser, V.; Sundblad, P.; Thonnard, J.-L.; Lefevre, P.; McIntyre, J.; Kassel, R.; Derkinderen, W.; Penta, M.; Andre, T.

    It has been shown that during exposure to microgravity in parabolic flights the control of interaction forces when manipulating an object adapts partially to the lack of gravity, yet evidence indicates that anticipation of gravity's effects persists in the short term. The motivation for these experiments to be performed in long-duration space flight is to understand how the central nervous system adapts to an environment without gravity and what will be the consequences of long-term adaptation when an individual returns to a normal (Earth) or partial (Moon or Mars) gravitational field. The experiment “Dexterous Manipulation in Microgravity” (DEX) will target specific questions about the effects of gravity on dexterous manipulation, questions that cannot be addressed in the normal terrestrial environment. Some of the scientific questions have already been studied since nearly ten years and will continue to be addressed in experiments conducted in parabolic flights, during which it will be examined how the nervous system copes with repeated transitions between different gravitational environments. Results from these experiments provide initial data about short-term adaptation to 0g. The experiments proposed for ISS draw from these short-term precursor experiments, but will emphasize long-term adaptation of sensorimotor processes to 0g and re-adaptation to 1g. A first conceptual definition phase of a DEX instrument has been completed under an ESA contract and is now ready to enter into the design and development phase in view of a launch on ISS in the 2013-2014 timeframe. In this paper, the science background will be recalled and several experiments performed during parabolic flights will be presented, showing how these early breadboards testing in microgravity have helped to refine the DEX conceptual design and how it could be used on ISS.

  12. Adenoviral transfer of mda-7 leads to BAX up-regulation and apoptosis in mesothelioma cells, and is abrogated by over-expression of BCL-XL.

    PubMed Central

    Cao, Xiaobo X.; Mohuiddin, Imran; Chada, Sunil; Mhashilkar, Abner M.; Ozvaran, Mustafa K.; McConkey, David J.; Miller, Steven D.; Daniel, Jonathon C.; Smythe, W. Roy

    2002-01-01

    BACKGROUND: Malignant pleural mesothelioma (MPM) is unresponsive to conventional therapies. Forced expression of the novel tumor suppressor mda-7 gene in other cell types has resulted in decreased growth and apoptosis. We evaluated cell growth, apoptosis and tumor suppressor characteristics following forced expression of this gene in mesothelioma cell lines. METHODS: MDA-7 expression in human MPM cells at baseline, following pharmacologic differentiation and viral mda-7 transduction (Ad-mda7) were evaluated with Western blot. Cell viability was evaluated with a colorimetric (XTT) assay, and apoptosis with subG1 FACS and Hoescht. Caspase-3 expression was evaluated by functional assay. These parameters were also evaluated in a stable bcl-xl hyper-expressing MPM cell line. Bax mRNA levels were evaluated with real-time PCR. RESULTS: No baseline or differentiated MPM MDA7 expression was found, but was noted following Ad-mda7 exposure. More than 50% of MPM cells were killed at 5 days following Ad-mda7 exposure (p < 0.001). Apoptosis was accompanied by caspase-3 cleavage and increased BAX expression at both the protein (translational) and mRNA (transcriptional) level. These findings were reduced in a bcl-xl hyper-expressing cell line (P < 0.01). CONCLUSIONS: Although mda-7 does not appear to be a MPM suppressor gene, adenoviral-mediated expression in cell lines induces apoptotic cellular death related to BAX upregulation and caspase cleavage. This is supported by abrogation of effect in a bcl-xl hyper-expressing cell line. PMID:12606823

  13. X-4 in flight

    NASA Technical Reports Server (NTRS)

    1951-01-01

    In the early days of transonic flight research, many aerodynamicists believed that eliminating conventional tail surfaces could reduce the problems created by shock wave interaction with the tail's lifting surfaces. To address this issue, the Army Air Forces's Air Technical Service awarded a contract to Northrop Aircraft Corporation on 5 April 1946 to build a piloted 'flying laboratory.' Northrop already had experience with tailless flying wing designs such as the N-1M, N-9M, XB-35, and YB-49. Subsequently, the manufacturer built two semi-tailless X-4 research aircraft, the first of which flew half a century ago. The X-4 was designed to investigate transonic compressibility effects at speeds near Mach 0.85 to 0.88, slightly below the speed of sound. Northrop project engineer Arthur Lusk designed the aircraft with swept wings and a conventional fuselage that housed two turbojet engines. It had a vertical stabilizer, but no horizontal tail surfaces. It was one of the smallest X-planes ever built, and every bit of internal space was used for systems and instrumentation. The first X-4 arrived at Muroc Air Force Base by truck on 15 November 1948. Over the course of several weeks, engineers conducted static tests, and Northrop test pilot Charles Tucker made initial taxi runs. Although small of stature, he barely fit into the diminutive craft. Tucker, a veteran Northrop test pilot, had previously flown the XB-35 and YB-49 flying wing bomber prototypes. Prior to flying for Northrop, he had logged 400 hours in jet airplanes as a test pilot for Lockheed and the Air Force. He would now be responsible for completing the contractor phase of the X-4 flight test program. Finally, all was ready. Tucker climbed into the cockpit, and made the first flight on 15 December 1948. It only lasted 18 minutes, allowing just enough time for the pilot to become familiar with the basic handling qualities of the craft. The X-4 handled well, but Tucker noted some longitudinal instability at all

  14. Accelerated and Synchronized Oviposition Induced by Flight of Young Females May Intensify Larval Outbreaks of the Rice Leaf Roller

    PubMed Central

    Zhang, Lei; Pan, Pan; Sappington, Thomas W.; Lu, Weixiang; Luo, Lizhi; Jiang, Xingfu

    2015-01-01

    Physiological management of migration-reproduction trade-offs in energy allocation often includes a package of adaptions referred to as the oogenesis-flight syndrome. In some species, this trade-off may be overestimated, because factors like flight behavior and environmental conditions may mitigate it. In this study, we examined the reproductive consequences induced by different flight scenarios in an economically-important Asian migrant insect, Cnaphalocrocis medinalis. We found that the influences of flight on reproduction are not absolutely positive or negative, but instead depend on the age at which the moth begins flight, flight duration, and how many consecutive nights they are flown. Adult flight on the 1st or 2nd night after emergence, flight for 6 h or 12 h nightly, and flight on the first two consecutive nights after emergence significantly accelerated onset of oviposition or enhanced synchrony of egg-laying. The latter can contribute to subsequent larval outbreaks. However, flight after the 3rd night, flight for 18 h at any age, or flight on more than 3 consecutive nights after adult emergence did not promote reproductive development, and in some scenarios even constrained adult reproduction. These results indicate that there is a migration/reproduction trade-off in C.medinalis, but that it is mitigated or eliminated by flight under appropriate conditions. The strategy of advanced and synchronized oviposition triggered by migratory flight of young females may be common in other migratory insect pests. PMID:25815767

  15. Evolutionary flight and enabling smart actuator devices

    NASA Astrophysics Data System (ADS)

    Manzo, Justin; Garcia, Ephrahim

    2007-04-01

    Recent interest in morphing vehicles with multiple, optimized configurations has led to renewed research on biological flight. The flying vertebrates - birds, bats, and pterosaurs - all made or make use of various morphing devices to achieve lift to suit rapidly changing flight demands, including maneuvers as complex as perching and hovering. The first part of this paper will discuss these devices, with a focus on the morphing elements and structural strong suits of each creature. Modern flight correlations to these devices will be discussed and analyzed as valid adaptations of these evolutionary traits. The second part of the paper will focus on the use of active joint structures for use in morphing aircraft devices. Initial work on smart actuator devices focused on NASA Langley's Hyper-Elliptical Cambered Span (HECS) wing platform, which led to development of a discretized spanwise curvature effector. This mechanism uses shape memory alloy (SMA) as the sole morphing actuator, allowing fast rotation with lightweight components at the expense of energy inefficiency. Phase two of morphing actuator development will add an element of active rigidity to the morphing structure, in the form of shape memory polymer (SMP). Employing a composite structure of polymer and alloy, this joint will function as part of a biomimetic morphing actuator system in a more energetically efficient manner. The joint is thermally actuated to allow compliance on demand and rigidity in the nominal configuration. Analytical and experimental joint models are presented, and potential applications on a bat-wing aircraft structure are outlined.

  16. Space Launch System Ascent Flight Control Design

    NASA Technical Reports Server (NTRS)

    Orr, Jeb S.; Wall, John H.; VanZwieten, Tannen S.; Hall, Charles E.

    2014-01-01

    A robust and flexible autopilot architecture for NASA's Space Launch System (SLS) family of launch vehicles is presented. The SLS configurations represent a potentially significant increase in complexity and performance capability when compared with other manned launch vehicles. It was recognized early in the program that a new, generalized autopilot design should be formulated to fulfill the needs of this new space launch architecture. The present design concept is intended to leverage existing NASA and industry launch vehicle design experience and maintain the extensibility and modularity necessary to accommodate multiple vehicle configurations while relying on proven and flight-tested control design principles for large boost vehicles. The SLS flight control architecture combines a digital three-axis autopilot with traditional bending filters to support robust active or passive stabilization of the vehicle's bending and sloshing dynamics using optimally blended measurements from multiple rate gyros on the vehicle structure. The algorithm also relies on a pseudo-optimal control allocation scheme to maximize the performance capability of multiple vectored engines while accommodating throttling and engine failure contingencies in real time with negligible impact to stability characteristics. The architecture supports active in-flight disturbance compensation through the use of nonlinear observers driven by acceleration measurements. Envelope expansion and robustness enhancement is obtained through the use of a multiplicative forward gain modulation law based upon a simple model reference adaptive control scheme.

  17. Economy of flight at supersonic speeds

    NASA Technical Reports Server (NTRS)

    Jones, R. T.

    1976-01-01

    Prandtl's theory is used to determine the airflow over bodies and wings adapted to supersonic flight. By making use of these results, and by incorporating in them an allowance for the probable skin friction, some estimates of expected lift-drag ratios are made for various flight speeds with the best configuration. At each speed a slender body and wings having the best angle of sweepback are considered. For the range of supersonic speeds shown an airplane of normal density and loading would be required to operate at an altitude of the order of 60,000 feet. The limiting value of 1-1/2 times the speed of sound corresponds to a flight speed of 1000 miles per hour. At this speed about 1.5 miles per gallon of fuel are expected. It is interesting to note that this value corresponds to a value of more than 15 miles per gallon when the weight is reduced to correspond to that of an ordinary automobile.

  18. Flight in slow motion: aerodynamics of the pterosaur wing

    PubMed Central

    Palmer, Colin

    2011-01-01

    The flight of pterosaurs and the extreme sizes of some taxa have long perplexed evolutionary biologists. Past reconstructions of flight capability were handicapped by the available aerodynamic data, which was unrepresentative of possible pterosaur wing profiles. I report wind tunnel tests on a range of possible pterosaur wing sections and quantify the likely performance for the first time. These sections have substantially higher profile drag and maximum lift coefficients than those assumed before, suggesting that large pterosaurs were aerodynamically less efficient and could fly more slowly than previously estimated. In order to achieve higher efficiency, the wing bones must be faired, which implies extensive regions of pneumatized tissue. Whether faired or not, the pterosaur wings were adapted to low-speed flight, unsuited to marine style dynamic soaring but adapted for thermal/slope soaring and controlled, low-speed landing. Because their thin-walled bones were susceptible to impact damage, slow flight would have helped to avoid injury and may have contributed to their attaining much larger sizes than fossil or extant birds. The trade-off would have been an extreme vulnerability to strong or turbulent winds both in flight and on the ground, akin to modern-day paragliders. PMID:21106584

  19. Flight in slow motion: aerodynamics of the pterosaur wing.

    PubMed

    Palmer, Colin

    2011-06-22

    The flight of pterosaurs and the extreme sizes of some taxa have long perplexed evolutionary biologists. Past reconstructions of flight capability were handicapped by the available aerodynamic data, which was unrepresentative of possible pterosaur wing profiles. I report wind tunnel tests on a range of possible pterosaur wing sections and quantify the likely performance for the first time. These sections have substantially higher profile drag and maximum lift coefficients than those assumed before, suggesting that large pterosaurs were aerodynamically less efficient and could fly more slowly than previously estimated. In order to achieve higher efficiency, the wing bones must be faired, which implies extensive regions of pneumatized tissue. Whether faired or not, the pterosaur wings were adapted to low-speed flight, unsuited to marine style dynamic soaring but adapted for thermal/slope soaring and controlled, low-speed landing. Because their thin-walled bones were susceptible to impact damage, slow flight would have helped to avoid injury and may have contributed to their attaining much larger sizes than fossil or extant birds. The trade-off would have been an extreme vulnerability to strong or turbulent winds both in flight and on the ground, akin to modern-day paragliders.

  20. Curcumin analog UBS109 prevents bone marrow osteoblastogenesis and osteoclastogenesis disordered by coculture with breast cancer MDA-MB-231 bone metastatic cells in vitro.

    PubMed

    Yamaguchi, Masayoshi; Zhu, Shijun; Weitzmann, M Neale; Snyder, James P; Shoji, Mamoru

    2015-03-01

    UBS109 is a curcumin analog that possesses antitumor properties has been shown to stimulate osteoblastogenesis and suppress osteoclastogenesis in vitro. This study was undertaken to determine whether UBS109 might alleviate the inhibitory activity of breast cancer cells on osteoblastic mineralization and stimulatory effects on osteoclastogenesis. Mouse bone marrow cells were cocultured with breast cancer MDA-MB-231 bone metastatic cells in vitro. UBS109 stimulated osteoblastic mineralization and suppressed adipogenesis and osteoclastogenesis in bone marrow culture. Coculture with MDA-MB-231 cells suppressed osteoblastic mineralization and enhanced osteoclastogenesis in bone marrow culture. Effects that were reserved by UBS109 (50-200 nM). Mineralization in preosteoblastic MC3T3-E1 cells was suppressed by coculture with MDA-MB-231 cells, while MDA-MB-231 cells did not have effects on osteoclastogenesis of RAW267.4 cells in vitro. UBS109 (500 nM) revealed toxic effects on MDA-MB-231 bone metastatic cells. This study demonstrates that UBS109, which is an antitumor agent, reveals restorative effects on bone marrow cell differentiation disordered by coculture with breast cancer MDA-MB-231 bone metastatic cells in vitro. This in vitro model may be a useful tool to evaluate the mechanism of breast cancer cell bone metastasis.

  1. Antagonism of the Phosphatase PP1 by the measles virus V protein is required for innate immune escape of MDA5

    PubMed Central

    Davis, Meredith E.; Wang, May K.; Rennick, Linda J.; Full, Florian; Gableske, Sebastian; Mesman, Annelies W.; Gringhuis, Sonja I.; Geijtenbeek, Teunis B.H.; Duprex, W. Paul; Gack, Michaela U.

    2014-01-01

    The cytosolic sensor MDA5 is crucial for antiviral innate immune defense against various RNA viruses including measles virus; as such, many viruses have evolved strategies to antagonize the antiviral activity of MDA5. Here, we show that measles virus escapes MDA5 detection by targeting the phosphatases PP1α and PP1γ, which regulate MDA5 activity by removing an inhibitory phosphorylation mark. The V proteins of measles virus and the related paramyxovirus Nipah virus interact with PP1α/γ, preventing PP1-mediated dephosphorylation of MDA5 and thereby its activation. The PP1 interaction with the measles V protein is mediated by a conserved PP1-binding motif in the C-terminal region of the V protein. A recombinant measles virus expressing a mutant V protein deficient in PP1 binding is unable to antagonize MDA5 and is growth-impaired due to its inability to suppress interferon induction. This identifies PP1 antagonism as a mechanism employed by paramyxoviruses for evading innate immune recognition. PMID:25011105

  2. Antagonism of the phosphatase PP1 by the measles virus V protein is required for innate immune escape of MDA5.

    PubMed

    Davis, Meredith E; Wang, May K; Rennick, Linda J; Full, Florian; Gableske, Sebastian; Mesman, Annelies W; Gringhuis, Sonja I; Geijtenbeek, Teunis B H; Duprex, W Paul; Gack, Michaela U

    2014-07-09

    The cytosolic sensor MDA5 is crucial for antiviral innate immune defense against various RNA viruses including measles virus; as such, many viruses have evolved strategies to antagonize the antiviral activity of MDA5. Here, we show that measles virus escapes MDA5 detection by targeting the phosphatases PP1α and PP1γ, which regulate MDA5 activity by removing an inhibitory phosphorylation mark. The V proteins of measles virus and the related paramyxovirus Nipah virus interact with PP1α/γ, preventing PP1-mediated dephosphorylation of MDA5 and thereby its activation. The PP1 interaction with the measles V protein is mediated by a conserved PP1-binding motif in the C-terminal region of the V protein. A recombinant measles virus expressing a mutant V protein deficient in PP1 binding is unable to antagonize MDA5 and is growth impaired due to its inability to suppress interferon induction. This identifies PP1 antagonism as a mechanism employed by paramyxoviruses for evading innate immune recognition.

  3. Canadian medical experiments on Shuttle Flight 41-G

    NASA Technical Reports Server (NTRS)

    Watt, D. G. D.; Money, K. E.; Bondar, R. L.; Thirsk, R. B.; Garneau, M.

    1985-01-01

    During the 41-G mission, two payload specialist astronauts took part in six Canadian medical experiments designed to measure how the human nervous system adapts to weightlessness, and how this might contribute to space motion sickness. Similar tests conducted pre-flight provided base-line data, and post-flight experiments examined re-adaptation to the ground. No changes were detected in the vestibulo-ocular reflex during this 8-day mission. Pronounced proprioceptive illusions were experienced, especially immediately post-flight. Tactile acuity was normal in the fingers and toes, but the ability to judge limb position was degraded. Estimates of the locations of familiar targets were grossly distorted in the absence of vision. There were no differences in taste thresholds or olfaction. Despite pre-flight tests showing unusual susceptibility to motion sickness, the Canadian payload specialist turned out to be less susceptible than normal on-orbit. Re-adaptation to the normal gravity environment occurred within the first day after landing.

  4. Locomotor Dysfunction after Long-Duration Space Flight and Development of Countermeasures to Facilitate Faster Recovery

    NASA Technical Reports Server (NTRS)

    Mulavara, A. P.; Wood, S. J.; Cohen, H. S.; Bloomberg, J. J.

    2012-01-01

    Exposure to the microgravity conditions of space flight induces adaptive modification in sensorimotor function allowing astronauts to operate in this unique environment. This adaptive state, however, is inappropriate for a 1-g environment. Consequently astronauts must spend time readapting to Earth s gravity following their return to Earth. During this readaptation period, alterations in sensorimotor function cause various disturbances in astronaut gait during postflight walking. They often rely more on vision for postural and gait stability and many report the need for greater cognitive supervision of motor actions that previous to space flight were fully automated. Over the last several years our laboratory has investigated postflight astronaut locomotion with the aim of better understanding how adaptive changes in underlying sensorimotor mechanisms contribute to postflight gait dysfunction. Exposure to the microgravity conditions of space flight induces adaptive modification in the control of vestibularly-mediated reflexive head movement during locomotion after space flight. Furthermore, during motor learning, adaptive transitions are composed of two main mechanisms: strategic and plastic. Strategic mechanisms represent immediate and transitory modifications in control to deal with changes in the prevailing environment that, if prolonged, induce plastic mechanisms designed to automate new behavioral responses. The goal of the present study was to examine the contributions of sensorimotor subsystems such as the vestibular and body load sensing (BLS) somatosensory influences on head movement control during locomotion after long-duration space flight. Further we present data on the two motor learning processes during readaptation of locomotor function after long-duration space flight.

  5. Cosmonauts' haemostasis system status before and after space flights

    NASA Astrophysics Data System (ADS)

    Kuzichkin, Dmitry; Markin, Andrey; Morukov, Boris

    indices was increased as compared with general population physiological norms. During the 1st day after long- and short-term flights a tendency for activation of coagulation system along inner and terminal pathways emerged (APTT, TT shortening, an increase in the SFMC concentration). After short-term space flights a tendency for activation of fibrin forming (an increase in the fibrin concentration) was evidenced, and, as a compensatory factor, for activation of fibrinolysis (an increase in fibrynolytic activity and D-dimer concentration). On the contrary, after long-term space flights, a tendency for fibrinolysys decline was observed (fibrinolytic activity and D-dimer concentration decreased at this the fibrinogen concentration remained virtually constant relative to the background level). During the 14th day of the post-flight period normalization of all studied parameters was observed. Discussion. After space flights a tendency for activation of haemostasis procoagulant component is observed. However, during short-term space flights compensatory systems become activated, which may be connected with developing of stress reactions of adaptation to weightlessness conditions and post-flight re-adaptation to ground conditions, while after long-term spaceflights the compensatory effect of fibrinolysis is not pronounced, possibly, due to metabolic process intensity reduction developing during long-duration stay in weightlessness conditions [Grigoriev A.I., Kaplansky A.S., Popova I.A., 1992]. Probably the relatively inactivated cosmonauts’ intrinsic pathway coagulation in pre-flight period (prolonged APTT) is one of the prerequisites of the high resistance to stress factors influence. Plausible this status of intrinsic pathway subject to consequent activation by adrenalin promotes body protection against thrombophilic tendency.

  6. Tight coordination of aerial flight maneuvers and sonar call production in insectivorous bats.

    PubMed

    Falk, Benjamin; Kasnadi, Joseph; Moss, Cynthia F

    2015-11-01

    Echolocating bats face the challenge of coordinating flight kinematics with the production of echolocation signals used to guide navigation. Previous studies of bat flight have focused on kinematics of fruit and nectar-feeding bats, often in wind tunnels with limited maneuvering, and without analysis of echolocation behavior. In this study, we engaged insectivorous big brown bats in a task requiring simultaneous turning and climbing flight, and used synchronized high-speed motion-tracking cameras and audio recordings to quantify the animals' coordination of wing kinematics and echolocation. Bats varied flight speed, turn rate, climb rate and wingbeat rate as they navigated around obstacles, and they adapted their sonar signals in patterning, duration and frequency in relation to the timing of flight maneuvers. We found that bats timed the emission of sonar calls with the upstroke phase of the wingbeat cycle in straight flight, and that this relationship changed when bats turned to navigate obstacles. We also characterized the unsteadiness of climbing and turning flight, as well as the relationship between speed and kinematic parameters. Adaptations in the bats' echolocation call frequency suggest changes in beam width and sonar field of view in relation to obstacles and flight behavior. By characterizing flight and sonar behaviors in an insectivorous bat species, we find evidence of exquisitely tight coordination of sensory and motor systems for obstacle navigation and insect capture.

  7. Tight coordination of aerial flight maneuvers and sonar call production in insectivorous bats

    PubMed Central

    Falk, Benjamin; Kasnadi, Joseph; Moss, Cynthia F.

    2015-01-01

    ABSTRACT Echolocating bats face the challenge of coordinating flight kinematics with the production of echolocation signals used to guide navigation. Previous studies of bat flight have focused on kinematics of fruit and nectar-feeding bats, often in wind tunnels with limited maneuvering, and without analysis of echolocation behavior. In this study, we engaged insectivorous big brown bats in a task requiring simultaneous turning and climbing flight, and used synchronized high-speed motion-tracking cameras and audio recordings to quantify the animals' coordination of wing kinematics and echolocation. Bats varied flight speed, turn rate, climb rate and wingbeat rate as they navigated around obstacles, and they adapted their sonar signals in patterning, duration and frequency in relation to the timing of flight maneuvers. We found that bats timed the emission of sonar calls with the upstroke phase of the wingbeat cycle in straight flight, and that this relationship changed when bats turned to navigate obstacles. We also characterized the unsteadiness of climbing and turning flight, as well as the relationship between speed and kinematic parameters. Adaptations in the bats' echolocation call frequency suggest changes in beam width and sonar field of view in relation to obstacles and flight behavior. By characterizing flight and sonar behaviors in an insectivorous bat species, we find evidence of exquisitely tight coordination of sensory and motor systems for obstacle navigation and insect capture. PMID:26582935

  8. The initial flight anomalies of Skylab 1

    NASA Technical Reports Server (NTRS)

    1993-01-01

    At approximately 63 seconds into the flight of Skylab 1 on May 14, 1973, an anomaly occurred which resulted in the complete loss of the meteoroid shield around the orbital workshop. This was followed by the loss of one of the two solar array systems on the workshop and a failure of the inter stage adapter to separate from the S-II stage of the Saturn V launch vehicle. The investigation reported herein identified the most probable cause of this flight anomaly to be the breakup and loss of the meteoroid shield due to aerodynamic loads that were not accounted for in its design. The breakup of the meteoroid shield, in turn, broke the tie downs that secured one of the solar array systems to the workshop. Complete loss of this solar array system occurred at 593 seconds when the exhaust plume of the S-II stage retro-rockets impacted the partially deployed solar array system. Falling debris from the meteoroid shield also damaged the S-II inter stage adapter ordnance system in such a manner as to preclude separation. Of several possible failure modes of the meteoroid shield that were identified, the most probable in this particular flight was internal pressurization of its auxiliary tunnel which acted to force the forward end of the meteoroid shield away from the shell of the workshop and into the supersonic air stream. The pressurization of the auxiliary tunnel was due to the existence of several openings in the aft region of the tunnel. Another possible failure mode was the separation of the leading edge of the meteoroid shield from the shell of the workshop (particularly in the region of the folded ordnance panel) of sufficient extent to admit ram air pressures under the shield.

  9. Cellular localization of the activated EGFR determines its effect on cell growth in MDA-MB-468 cells

    SciTech Connect

    Hyatt, Dustin C.; Ceresa, Brian P.

    2008-11-01

    The epidermal growth factor (EGF) receptor (EGFR) is a ubiquitously expressed receptor tyrosine kinase that regulates diverse cell functions that are dependent upon cell type, the presence of downstream effectors, and receptor density. In addition to activating biochemical pathways, ligand stimulation causes the EGFR to enter the cell via clathrin-coated pits. Endocytic trafficking influences receptor signaling by controlling the duration of EGFR phosphorylation and coordinating the receptor's association with downstream effectors. To better understand the individual contributions of cell surface and cytosolic EGFRs on cell physiology, we used EGF that was conjugated to 900 nm polystyrene beads (EGF-beads). EGF-beads can stimulate the EGFR and retain the activated receptor at the plasma membrane. In MDA-MB-468 cells, a breast cancer cell line that over-expresses the EGFR, only internalized, activated EGFRs stimulate caspase-3 and induce cell death. Conversely, signaling cascades triggered from activated EGFR retained at the cell surface inhibit caspase-3 and promote cell proliferation. Thus, through endocytosis, the activated EGFR can differentially regulate cell growth in MDA-MB-468 cells.

  10. Broccoli and watercress suppress matrix metalloproteinase-9 activity and invasiveness of human MDA-MB-231 breast cancer cells

    SciTech Connect

    Rose, Peter . E-mail: bchpcr@nus.edu.sg; Huang, Qing; Ong, Choon Nam; Whiteman, Matt

    2005-12-01

    A high dietary intake of cruciferous vegetables has been associated with a reduction in numerous human pathologies particularly cancer. In the current study, we examined the inhibitory effects of broccoli (Brassica oleracea var. italica) and watercress (Rorripa nasturtium aquaticum) extracts on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cancer cell invasion and matrix metalloproteinase-9 activity using human MDA-MB-231 breast cancer cells. Aberrant overexpression of matrix metalloproteinases, including metalloproteinase-9, is associated with increased invasive potential in cancer cell lines. Our results demonstrate that extracts of broccoli and Rorripa suppressed TPA-induced MMP-9 activity and invasiveness in a concentration dependant manner as determined by zymographic analysis. Furthermore, fractionation of individual extracts followed by liquid chromatography mass spectroscopy analysis (LC-MS) revealed that the inhibitory effects of each vegetable were associated with the presence of 4-methysulfinylbutyl (sulforaphane) and 7-methylsulphinylheptyl isothiocyanates. Taken together, our data indicate that isothiocyanates derived form broccoli and Rorripa inhibit metalloproteinase 9 activities and also suppress the invasive potential of human MDA-MB-231 breast cancer cells in vitro. The inhibitory effects observed in the current study may contribute to the suppression of carcinogenesis by diets high in cruciferous vegetables.

  11. Broccoli and watercress suppress matrix metalloproteinase-9 activity and invasiveness of human MDA-MB-231 breast cancer cells.

    PubMed

    Rose, Peter; Huang, Qing; Ong, Choon Nam; Whiteman, Matt

    2005-12-01

    A high dietary intake of cruciferous vegetables has been associated with a reduction in numerous human pathologies particularly cancer. In the current study, we examined the inhibitory effects of broccoli (Brassica oleracea var. italica) and watercress (Rorripa nasturtium aquaticum) extracts on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cancer cell invasion and matrix metalloproteinase-9 activity using human MDA-MB-231 breast cancer cells. Aberrant overexpression of matrix metalloproteinases, including metalloproteinase-9, is associated with increased invasive potential in cancer cell lines. Our results demonstrate that extracts of broccoli and Rorripa suppressed TPA-induced MMP-9 activity and invasiveness in a concentration dependent manner as determined by zymographic analysis. Furthermore, fractionation of individual extracts followed by liquid chromatography mass spectroscopy analysis (LC-MS) revealed that the inhibitory effects of each vegetable were associated with the presence of 4-methysulfinylbutyl (sulforaphane) and 7-methylsulphinylheptyl isothiocyanates. Taken together, our data indicate that isothiocyanates derived form broccoli and Rorripa inhibit metalloproteinase 9 activities and also suppress the invasive potential of human MDA-MB-231 breast cancer cells in vitro. The inhibitory effects observed in the current study may contribute to the suppression of carcinogenesis by diets high in cruciferous vegetables.

  12. The FGFR4 Y367C mutant is a dominant oncogene in MDA-MB453 breast cancer cells.

    PubMed

    Roidl, A; Foo, P; Wong, W; Mann, C; Bechtold, S; Berger, H J; Streit, S; Ruhe, J E; Hart, S; Ullrich, A; Ho, H K

    2010-03-11

    Mutational analysis of oncogenes is critical for our understanding of cancer development. Oncogenome screening has identified a fibroblast growth factor receptor 4 (FGFR4) Y367C mutation in the human breast cancer cell line MDA-MB453. Here, we investigate the consequence of this missense mutation in cancer cells. We show that MDA-MB453 cells harbouring the mutation are insensitive to FGFR4-specific ligand stimulation or inhibition with an antagonistic antibody. Furthermore, the FGFR4 mutant elicits constitutive phosphorylation leading to an activation of the mitogen-activated protein kinase cascade as shown by an enhanced Erk1/2 phosphorylation. Cloning and ectopic expression of the FGFR4 Y367C mutant in HEK293 cells revealed high pErk levels and enhanced cell proliferation. Based on these findings, we propose that FGFR4 may be a driver of tumour growth, particularly when highly expressed or stabilized and constitutively activated through genetic alterations. As such, FGFR4 presents an option for further mutational screening in tumours and is an attractive cancer target with the therapeutic potential.

  13. FGFR4 GLY388 isotype suppresses motility of MDA-MB-231 breast cancer cells by EDG-2 gene repression.

    PubMed

    Stadler, Christiane Regina; Knyazev, Pjotr; Bange, Johannes; Ullrich, Axel

    2006-06-01

    Clinical investigations of an FGFR4 germline polymorphism, resulting in substitution of glycine by arginine at codon 388 (G388 to R388), have shown a correlation between FGFR4 R388 and aggressive disease progression in cancer patients. Here, we studied the differential effects of the two FGFR4 isotypes on cellular signalling and motility in the MDA-MB-231 human breast cancer cell model. cDNA array analysis showed the ability of FGFR4 G388 to suppress expression of specific genes involved in invasiveness and motility. Further investigations concentrating on cell signalling and motility revealed an abrogation of phosphatidylinositol-3-kinase-dependent LPA-induced Akt activation and cell migration due to downregulation of the LPA receptor Edg-2 in FGFR4 G388-expressing MDA-MB-231 cells. Moreover, FGFR4 G388 expression attenuated the invasivity of the breast cancer cell line and decreased small Rho GTPase activity. We conclude that FGFR4 G388 suppresses cell motility of invasive breast cancer cells by altering signalling pathways and the expression of genes that are required for metastasis. Therefore, the positive effect of FGFR4 R388 on disease progression appears to result from a loss of the tumour suppressor activity displayed by FGFR4 G388 rather than the acquisition or enhancement of oncogenic potential.

  14. The 737 graphite composite flight spoiler flight service evaluation

    NASA Technical Reports Server (NTRS)

    Coggeshall, R. L.

    1985-01-01

    The flight service experience of 111 graphite-epoxy spoilers on 737 transport aircraft and related ground based environmental exposure of graphite-epoxy material specimens is covered. Spoilers have been installed on 28 aircraft representing seven major airlines operating throughout the world. An extended flight service evaluation program of 15 years is presently underway. As of December 1984, a total of 2,092, 155 spoiler flight hours and 2,954,814 spoiler landings had been accumulated by this fleet.

  15. Orion Pad Abort 1 Flight Test - Ground and Flight Operations

    NASA Technical Reports Server (NTRS)

    Hackenbergy, Davis L.; Hicks, Wayne

    2011-01-01

    This paper discusses the ground and flight operations aspects to the Pad Abort 1 launch. The paper details the processes used to plan all operations. The paper then discussions the difficulties of integration and testing, while detailing some of the lessons learned throughout the entire launch campaign. Flight operational aspects of the launc are covered in order to provide the listener with the full suite of operational issues encountered in preparation for the first flight test of the Orion Launch Abort System.

  16. Flight test of a propulsion controlled aircraft system on the NASA F-15 airplane

    NASA Technical Reports Server (NTRS)

    Burcham, Frank W., Jr.; Maine, Trindel A.

    1995-01-01

    Flight tests of the propulsion controlled aircraft (PCA) system on the NASA F-15 airplane evolved as a result of a long series of simulation and flight tests. Initially, the simulation results were very optimistic. Early flight tests showed that manual throttles-only control was much more difficult than the simulation, and a flight investigation was flown to acquire data to resolve this discrepancy. The PCA system designed and developed by MDA evolved as these discrepancies were found and resolved, requiring redesign of the PCA software and modification of the flight test plan. Small throttle step inputs were flown to provide data for analysis, simulation update, and control logic modification. The PCA flight tests quickly revealed less than desired performance, but the extensive flexibility built into the flight PCA software allowed rapid evaluation of alternate gains, filters, and control logic, and within 2 weeks, the PCA system was functioning well. The initial objective of achieving adequate control for up-and-away flying and approaches was satisfied, and the option to continue to actual landings was achieved. After the PCA landings were accomplished, other PCA features were added, and additional maneuvers beyond those originally planned were flown. The PCA system was used to recover from extreme upset conditions, descend, and make approaches to landing. A heading mode was added, and a single engine plus rudder PCA mode was also added and flown. The PCA flight envelope was expanded far beyond that originally designed for. Guest pilots from the USAF, USN, NASA, and the contractor also flew the PCA system and were favorably impressed.

  17. Toothbrush Adaptations.

    ERIC Educational Resources Information Center

    Exceptional Parent, 1987

    1987-01-01

    Suggestions are presented for helping disabled individuals learn to use or adapt toothbrushes for proper dental care. A directory lists dental health instructional materials available from various organizations. (CB)

  18. Visualization of In-Flight Flow Phenomena Using Infrared Thermography

    NASA Technical Reports Server (NTRS)

    Banks, D. W.; vanDam, C. P.; Shiu, H. J.; Miller, G. M.

    2000-01-01

    Infrared thermography was used to obtain data on the state of the boundary layer of a natural laminar flow airfoil in supersonic flight. In addition to the laminar-to-turbulent transition boundary, the infrared camera was able to detect shock waves and present a time dependent view of the flow field. A time dependent heat transfer code was developed to predict temperature distributions on the test subject and any necessary surface treatment. A commercially available infrared camera was adapted for airborne use in this application. Readily available infrared technology has the capability to provide detailed visualization of various flow phenomena in subsonic to hypersonic flight regimes.

  19. Modern digital flight control system design for VTOL aircraft

    NASA Technical Reports Server (NTRS)

    Broussard, J. R.; Berry, P. W.; Stengel, R. F.

    1979-01-01

    Methods for and results from the design and evaluation of a digital flight control system (DFCS) for a CH-47B helicopter are presented. The DFCS employed proportional-integral control logic to provide rapid, precise response to automatic or manual guidance commands while following conventional or spiral-descent approach paths. It contained altitude- and velocity-command modes, and it adapted to varying flight conditions through gain scheduling. Extensive use was made of linear systems analysis techniques. The DFCS was designed, using linear-optimal estimation and control theory, and the effects of gain scheduling are assessed by examination of closed-loop eigenvalues and time responses.

  20. The 737 graphite composite flight spoiler flight service evaluation

    NASA Technical Reports Server (NTRS)

    Coggeshall, Randy L.

    1987-01-01

    The ninth flight service report was prepared in compliance with the requirements of Contract NAS1-11668. It covers the flight service experience of 111 graphite-epoxy spoilers on 737 transport aircraft and related ground-based environmental exposure of graphite epoxy material specimens for the period 1 Jan. 1985 through 31 Dec. 1986. Spoilers have been installed on 28 aircraft representing seven major airlines operating throughout the world. An extended flight service evaluation program of 15 years is presently underway. As of 31 Dec. 1986, a total of 3,339,608 spoiler flight-hours and 3,320,952 spoiler landings had been accumulated by this fleet.