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Sample records for adaptive optics images

  1. Retinal Imaging: Adaptive Optics

    NASA Astrophysics Data System (ADS)

    Goncharov, A. S.; Iroshnikov, N. G.; Larichev, Andrey V.

    This chapter describes several factors influencing the performance of ophthalmic diagnostic systems with adaptive optics compensation of human eye aberration. Particular attention is paid to speckle modulation, temporal behavior of aberrations, and anisoplanatic effects. The implementation of a fundus camera with adaptive optics is considered.

  2. Adaptive optical ghost imaging through atmospheric turbulence.

    PubMed

    Shi, Dongfeng; Fan, Chengyu; Zhang, Pengfei; Zhang, Jinghui; Shen, Hong; Qiao, Chunhong; Wang, Yingjian

    2012-12-17

    We demonstrate for the first time (to our knowledge) that a high-quality image can still be obtained in atmospheric turbulence by applying adaptive optical ghost imaging (AOGI) system even when conventional ghost imaging system fails to produce an image. The performance of AOGI under different strength of atmospheric turbulence is investigated by simulation. The influence of adaptive optics system with different numbers of adaptive mirror elements on obtained image quality is also studied.

  3. Adaptive optics imaging of the retina.

    PubMed

    Battu, Rajani; Dabir, Supriya; Khanna, Anjani; Kumar, Anupama Kiran; Roy, Abhijit Sinha

    2014-01-01

    Adaptive optics is a relatively new tool that is available to ophthalmologists for study of cellular level details. In addition to the axial resolution provided by the spectral-domain optical coherence tomography, adaptive optics provides an excellent lateral resolution, enabling visualization of the photoreceptors, blood vessels and details of the optic nerve head. We attempt a mini review of the current role of adaptive optics in retinal imaging. PubMed search was performed with key words Adaptive optics OR Retina OR Retinal imaging. Conference abstracts were searched from the Association for Research in Vision and Ophthalmology (ARVO) and American Academy of Ophthalmology (AAO) meetings. In total, 261 relevant publications and 389 conference abstracts were identified.

  4. Adaptive optics imaging of the retina

    PubMed Central

    Battu, Rajani; Dabir, Supriya; Khanna, Anjani; Kumar, Anupama Kiran; Roy, Abhijit Sinha

    2014-01-01

    Adaptive optics is a relatively new tool that is available to ophthalmologists for study of cellular level details. In addition to the axial resolution provided by the spectral-domain optical coherence tomography, adaptive optics provides an excellent lateral resolution, enabling visualization of the photoreceptors, blood vessels and details of the optic nerve head. We attempt a mini review of the current role of adaptive optics in retinal imaging. PubMed search was performed with key words Adaptive optics OR Retina OR Retinal imaging. Conference abstracts were searched from the Association for Research in Vision and Ophthalmology (ARVO) and American Academy of Ophthalmology (AAO) meetings. In total, 261 relevant publications and 389 conference abstracts were identified. PMID:24492503

  5. Adaptive optics retinal imaging: emerging clinical applications.

    PubMed

    Godara, Pooja; Dubis, Adam M; Roorda, Austin; Duncan, Jacque L; Carroll, Joseph

    2010-12-01

    The human retina is a uniquely accessible tissue. Tools like scanning laser ophthalmoscopy and spectral domain-optical coherence tomography provide clinicians with remarkably clear pictures of the living retina. Although the anterior optics of the eye permit such non-invasive visualization of the retina and associated pathology, the same optics induce significant aberrations that obviate cellular-resolution imaging in most cases. Adaptive optics (AO) imaging systems use active optical elements to compensate for aberrations in the optical path between the object and the camera. When applied to the human eye, AO allows direct visualization of individual rod and cone photoreceptor cells, retinal pigment epithelium cells, and white blood cells. AO imaging has changed the way vision scientists and ophthalmologists see the retina, helping to clarify our understanding of retinal structure, function, and the etiology of various retinal pathologies. Here, we review some of the advances that were made possible with AO imaging of the human retina and discuss applications and future prospects for clinical imaging.

  6. Adaptive Optics Imaging in Laser Pointer Maculopathy.

    PubMed

    Sheyman, Alan T; Nesper, Peter L; Fawzi, Amani A; Jampol, Lee M

    2016-08-01

    The authors report multimodal imaging including adaptive optics scanning laser ophthalmoscopy (AOSLO) (Apaeros retinal image system AOSLO prototype; Boston Micromachines Corporation, Boston, MA) in a case of previously diagnosed unilateral acute idiopathic maculopathy (UAIM) that demonstrated features of laser pointer maculopathy. The authors also show the adaptive optics images of a laser pointer maculopathy case previously reported. A 15-year-old girl was referred for the evaluation of a maculopathy suspected to be UAIM. The authors reviewed the patient's history and obtained fluorescein angiography, autofluorescence, optical coherence tomography, infrared reflectance, and AOSLO. The time course of disease and clinical examination did not fit with UAIM, but the linear pattern of lesions was suspicious for self-inflicted laser pointer injury. This was confirmed on subsequent questioning of the patient. The presence of linear lesions in the macula that are best highlighted with multimodal imaging techniques should alert the physician to the possibility of laser pointer injury. AOSLO further characterizes photoreceptor damage in this condition. [Ophthalmic Surg Lasers Imaging Retina. 2016;47:782-785.]. PMID:27548458

  7. Adaptive Optics Imaging in Laser Pointer Maculopathy.

    PubMed

    Sheyman, Alan T; Nesper, Peter L; Fawzi, Amani A; Jampol, Lee M

    2016-08-01

    The authors report multimodal imaging including adaptive optics scanning laser ophthalmoscopy (AOSLO) (Apaeros retinal image system AOSLO prototype; Boston Micromachines Corporation, Boston, MA) in a case of previously diagnosed unilateral acute idiopathic maculopathy (UAIM) that demonstrated features of laser pointer maculopathy. The authors also show the adaptive optics images of a laser pointer maculopathy case previously reported. A 15-year-old girl was referred for the evaluation of a maculopathy suspected to be UAIM. The authors reviewed the patient's history and obtained fluorescein angiography, autofluorescence, optical coherence tomography, infrared reflectance, and AOSLO. The time course of disease and clinical examination did not fit with UAIM, but the linear pattern of lesions was suspicious for self-inflicted laser pointer injury. This was confirmed on subsequent questioning of the patient. The presence of linear lesions in the macula that are best highlighted with multimodal imaging techniques should alert the physician to the possibility of laser pointer injury. AOSLO further characterizes photoreceptor damage in this condition. [Ophthalmic Surg Lasers Imaging Retina. 2016;47:782-785.].

  8. Approach for reconstructing anisoplanatic adaptive optics images.

    PubMed

    Aubailly, Mathieu; Roggemann, Michael C; Schulz, Timothy J

    2007-08-20

    Atmospheric turbulence corrupts astronomical images formed by ground-based telescopes. Adaptive optics systems allow the effects of turbulence-induced aberrations to be reduced for a narrow field of view corresponding approximately to the isoplanatic angle theta(0). For field angles larger than theta(0), the point spread function (PSF) gradually degrades as the field angle increases. We present a technique to estimate the PSF of an adaptive optics telescope as function of the field angle, and use this information in a space-varying image reconstruction technique. Simulated anisoplanatic intensity images of a star field are reconstructed by means of a block-processing method using the predicted local PSF. Two methods for image recovery are used: matrix inversion with Tikhonov regularization, and the Lucy-Richardson algorithm. Image reconstruction results obtained using the space-varying predicted PSF are compared to space invariant deconvolution results obtained using the on-axis PSF. The anisoplanatic reconstruction technique using the predicted PSF provides a significant improvement of the mean squared error between the reconstructed image and the object compared to the deconvolution performed using the on-axis PSF. PMID:17712366

  9. Adaptive Optics Imaging of Solar System Objects

    NASA Technical Reports Server (NTRS)

    Roddier, Francois; Owen, Toby

    1997-01-01

    Most solar system objects have never been observed at wavelengths longer than the R band with an angular resolution better than 1 sec. The Hubble Space Telescope itself has only recently been equipped to observe in the infrared. However, because of its small diameter, the angular resolution is lower than that one can now achieved from the ground with adaptive optics, and time allocated to planetary science is limited. We have been using adaptive optics (AO) on a 4-m class telescope to obtain 0.1 sec resolution images solar system objects at far red and near infrared wavelengths (0.7-2.5 micron) which best discriminate their spectral signatures. Our efforts has been put into areas of research for which high angular resolution is essential, such as the mapping of Titan and of large asteroids, the dynamics and composition of Neptune stratospheric clouds, the infrared photometry of Pluto, Charon, and close satellites previously undetected from the ground.

  10. Adaptive Optics Imaging of Solar System Objects

    NASA Technical Reports Server (NTRS)

    Roddier, Francois; Owen, Toby

    1999-01-01

    Most solar system objects have never been observed at wavelengths longer than the R band with an angular resolution better than 1". The Hubble Space Telescope itself has only recently been equipped to observe in the infrared. However, because of its small diameter, the angular resolution is lower than that one can now achieved from the ground with adaptive optics, and time allocated to planetary science is limited. We have successfully used adaptive optics on a 4-m class telescope to obtain 0.1" resolution images of solar system objects in the far red and near infrared (0.7-2.5 microns), aE wavelengths which best discl"lmlnate their spectral signatures. Our efforts have been put into areas of research for which high angular resolution is essential.

  11. Retinal imaging using adaptive optics technology☆

    PubMed Central

    Kozak, Igor

    2014-01-01

    Adaptive optics (AO) is a technology used to improve the performance of optical systems by reducing the effect of wave front distortions. Retinal imaging using AO aims to compensate for higher order aberrations originating from the cornea and the lens by using deformable mirror. The main application of AO retinal imaging has been to assess photoreceptor cell density, spacing, and mosaic regularity in normal and diseased eyes. Apart from photoreceptors, the retinal pigment epithelium, retinal nerve fiber layer, retinal vessel wall and lamina cribrosa can also be visualized with AO technology. Recent interest in AO technology in eye research has resulted in growing number of reports and publications utilizing this technology in both animals and humans. With the availability of first commercially available instruments we are making transformation of AO technology from a research tool to diagnostic instrument. The current challenges include imaging eyes with less than perfect optical media, formation of normative databases for acquired images such as cone mosaics, and the cost of the technology. The opportunities for AO will include more detailed diagnosis with description of some new findings in retinal diseases and glaucoma as well as expansion of AO into clinical trials which has already started. PMID:24843304

  12. Retinal imaging using adaptive optics technology.

    PubMed

    Kozak, Igor

    2014-04-01

    Adaptive optics (AO) is a technology used to improve the performance of optical systems by reducing the effect of wave front distortions. Retinal imaging using AO aims to compensate for higher order aberrations originating from the cornea and the lens by using deformable mirror. The main application of AO retinal imaging has been to assess photoreceptor cell density, spacing, and mosaic regularity in normal and diseased eyes. Apart from photoreceptors, the retinal pigment epithelium, retinal nerve fiber layer, retinal vessel wall and lamina cribrosa can also be visualized with AO technology. Recent interest in AO technology in eye research has resulted in growing number of reports and publications utilizing this technology in both animals and humans. With the availability of first commercially available instruments we are making transformation of AO technology from a research tool to diagnostic instrument. The current challenges include imaging eyes with less than perfect optical media, formation of normative databases for acquired images such as cone mosaics, and the cost of the technology. The opportunities for AO will include more detailed diagnosis with description of some new findings in retinal diseases and glaucoma as well as expansion of AO into clinical trials which has already started. PMID:24843304

  13. Extreme Adaptive Optics Planet Imager: XAOPI

    SciTech Connect

    Macintosh, B A; Graham, J; Poyneer, L; Sommargren, G; Wilhelmsen, J; Gavel, D; Jones, S; Kalas, P; Lloyd, J; Makidon, R; Olivier, S; Palmer, D; Patience, J; Perrin, M; Severson, S; Sheinis, A; Sivaramakrishnan, A; Troy, M; Wallace, K

    2003-09-17

    Ground based adaptive optics is a potentially powerful technique for direct imaging detection of extrasolar planets. Turbulence in the Earth's atmosphere imposes some fundamental limits, but the large size of ground-based telescopes compared to spacecraft can work to mitigate this. We are carrying out a design study for a dedicated ultra-high-contrast system, the eXtreme Adaptive Optics Planet Imager (XAOPI), which could be deployed on an 8-10m telescope in 2007. With a 4096-actuator MEMS deformable mirror it should achieve Strehl >0.9 in the near-IR. Using an innovative spatially filtered wavefront sensor, the system will be optimized to control scattered light over a large radius and suppress artifacts caused by static errors. We predict that it will achieve contrast levels of 10{sup 7}-10{sup 8} at angular separations of 0.2-0.8 inches around a large sample of stars (R<7-10), sufficient to detect Jupiter-like planets through their near-IR emission over a wide range of ages and masses. We are constructing a high-contrast AO testbed to verify key concepts of our system, and present preliminary results here, showing an RMS wavefront error of <1.3 nm with a flat mirror.

  14. Adaptive Optics Imaging and Spectroscopy of Neptune

    NASA Technical Reports Server (NTRS)

    Johnson, Lindley (Technical Monitor); Sromovsky, Lawrence A.

    2005-01-01

    OBJECTIVES: We proposed to use high spectral resolution imaging and spectroscopy of Neptune in visible and near-IR spectral ranges to advance our understanding of Neptune s cloud structure. We intended to use the adaptive optics (AO) system at Mt. Wilson at visible wavelengths to try to obtain the first groundbased observations of dark spots on Neptune; we intended to use A 0 observations at the IRTF to obtain near-IR R=2000 spatially resolved spectra and near-IR A0 observations at the Keck observatory to obtain the highest spatial resolution studies of cloud feature dynamics and atmospheric motions. Vertical structure of cloud features was to be inferred from the wavelength dependent absorption of methane and hydrogen,

  15. Adaptive Optics Imaging of Exoplanet Host Stars

    NASA Astrophysics Data System (ADS)

    Herman, Miranda; Waaler, Mason; Patience, Jennifer; Ward-Duong, Kimberly; Rajan, Abhijith; McCarthy, Don; Kulesa, Craig; Wilson, Paul A.

    2016-01-01

    With the Arizona Infrared imager and Echelle Spectrograph (ARIES) instrument on the 6.5m MMT telescope, we obtained high angular resolution adaptive optics images of 12 exoplanet host stars. The targets are all systems with exoplanets in extremely close orbits such that the planets transit the host stars and cause regular brightness changes in the stars. The transit depth of the light curve is used to infer the radius and, in combination with radial velocity measurements, the density of the planet, but the results can be biased if the light from the host star is the combined light of a pair of stars in a binary system or a chance alignment of two stars. Given the high frequency of binary star systems and the increasing number of transit exoplanet discoveries from Kepler, K2, and anticipated discoveries with the Transiting Exoplanet Survey Satellite (TESS), this is a crucial point to consider when interpreting exoplanet properties. Companions were identified around five of the twelve targets at separations close enough that the brightness measurements of these host stars are in fact the combined brightness of two stars. Images of the resolved stellar systems and reanalysis of the exoplanet properties accounting for the presence of two stars are presented.

  16. Speckle image reconstruction of the adaptive optics solar images.

    PubMed

    Zhong, Libo; Tian, Yu; Rao, Changhui

    2014-11-17

    Speckle image reconstruction, in which the speckle transfer function (STF) is modeled as annular distribution according to the angular dependence of adaptive optics (AO) compensation and the individual STF in each annulus is obtained by the corresponding Fried parameter calculated from the traditional spectral ratio method, is used to restore the solar images corrected by AO system in this paper. The reconstructions of the solar images acquired by a 37-element AO system validate this method and the image quality is improved evidently. Moreover, we found the photometric accuracy of the reconstruction is field dependent due to the influence of AO correction. With the increase of angular separation of the object from the AO lockpoint, the relative improvement becomes approximately more and more effective and tends to identical in the regions far away the central field of view. The simulation results show this phenomenon is mainly due to the disparity of the calculated STF from the real AO STF with the angular dependence.

  17. Adaptive optics and phase diversity imaging for responsive space applications.

    SciTech Connect

    Smith, Mark William; Wick, David Victor

    2004-11-01

    The combination of phase diversity and adaptive optics offers great flexibility. Phase diverse images can be used to diagnose aberrations and then provide feedback control to the optics to correct the aberrations. Alternatively, phase diversity can be used to partially compensate for aberrations during post-detection image processing. The adaptive optic can produce simple defocus or more complex types of phase diversity. This report presents an analysis, based on numerical simulations, of the efficiency of different modes of phase diversity with respect to compensating for specific aberrations during post-processing. It also comments on the efficiency of post-processing versus direct aberration correction. The construction of a bench top optical system that uses a membrane mirror as an active optic is described. The results of characterization tests performed on the bench top optical system are presented. The work described in this report was conducted to explore the use of adaptive optics and phase diversity imaging for responsive space applications.

  18. Coherence-Gated Sensorless Adaptive Optics Multiphoton Retinal Imaging.

    PubMed

    Cua, Michelle; Wahl, Daniel J; Zhao, Yuan; Lee, Sujin; Bonora, Stefano; Zawadzki, Robert J; Jian, Yifan; Sarunic, Marinko V

    2016-09-07

    Multiphoton microscopy enables imaging deep into scattering tissues. The efficient generation of non-linear optical effects is related to both the pulse duration (typically on the order of femtoseconds) and the size of the focused spot. Aberrations introduced by refractive index inhomogeneity in the sample distort the wavefront and enlarge the focal spot, which reduces the multiphoton signal. Traditional approaches to adaptive optics wavefront correction are not effective in thick or multi-layered scattering media. In this report, we present sensorless adaptive optics (SAO) using low-coherence interferometric detection of the excitation light for depth-resolved aberration correction of two-photon excited fluorescence (TPEF) in biological tissue. We demonstrate coherence-gated SAO TPEF using a transmissive multi-actuator adaptive lens for in vivo imaging in a mouse retina. This configuration has significant potential for reducing the laser power required for adaptive optics multiphoton imaging, and for facilitating integration with existing systems.

  19. Coherence-Gated Sensorless Adaptive Optics Multiphoton Retinal Imaging

    NASA Astrophysics Data System (ADS)

    Cua, Michelle; Wahl, Daniel J.; Zhao, Yuan; Lee, Sujin; Bonora, Stefano; Zawadzki, Robert J.; Jian, Yifan; Sarunic, Marinko V.

    2016-09-01

    Multiphoton microscopy enables imaging deep into scattering tissues. The efficient generation of non-linear optical effects is related to both the pulse duration (typically on the order of femtoseconds) and the size of the focused spot. Aberrations introduced by refractive index inhomogeneity in the sample distort the wavefront and enlarge the focal spot, which reduces the multiphoton signal. Traditional approaches to adaptive optics wavefront correction are not effective in thick or multi-layered scattering media. In this report, we present sensorless adaptive optics (SAO) using low-coherence interferometric detection of the excitation light for depth-resolved aberration correction of two-photon excited fluorescence (TPEF) in biological tissue. We demonstrate coherence-gated SAO TPEF using a transmissive multi-actuator adaptive lens for in vivo imaging in a mouse retina. This configuration has significant potential for reducing the laser power required for adaptive optics multiphoton imaging, and for facilitating integration with existing systems.

  20. Coherence-Gated Sensorless Adaptive Optics Multiphoton Retinal Imaging.

    PubMed

    Cua, Michelle; Wahl, Daniel J; Zhao, Yuan; Lee, Sujin; Bonora, Stefano; Zawadzki, Robert J; Jian, Yifan; Sarunic, Marinko V

    2016-01-01

    Multiphoton microscopy enables imaging deep into scattering tissues. The efficient generation of non-linear optical effects is related to both the pulse duration (typically on the order of femtoseconds) and the size of the focused spot. Aberrations introduced by refractive index inhomogeneity in the sample distort the wavefront and enlarge the focal spot, which reduces the multiphoton signal. Traditional approaches to adaptive optics wavefront correction are not effective in thick or multi-layered scattering media. In this report, we present sensorless adaptive optics (SAO) using low-coherence interferometric detection of the excitation light for depth-resolved aberration correction of two-photon excited fluorescence (TPEF) in biological tissue. We demonstrate coherence-gated SAO TPEF using a transmissive multi-actuator adaptive lens for in vivo imaging in a mouse retina. This configuration has significant potential for reducing the laser power required for adaptive optics multiphoton imaging, and for facilitating integration with existing systems. PMID:27599635

  1. Coherence-Gated Sensorless Adaptive Optics Multiphoton Retinal Imaging

    PubMed Central

    Cua, Michelle; Wahl, Daniel J.; Zhao, Yuan; Lee, Sujin; Bonora, Stefano; Zawadzki, Robert J.; Jian, Yifan; Sarunic, Marinko V.

    2016-01-01

    Multiphoton microscopy enables imaging deep into scattering tissues. The efficient generation of non-linear optical effects is related to both the pulse duration (typically on the order of femtoseconds) and the size of the focused spot. Aberrations introduced by refractive index inhomogeneity in the sample distort the wavefront and enlarge the focal spot, which reduces the multiphoton signal. Traditional approaches to adaptive optics wavefront correction are not effective in thick or multi-layered scattering media. In this report, we present sensorless adaptive optics (SAO) using low-coherence interferometric detection of the excitation light for depth-resolved aberration correction of two-photon excited fluorescence (TPEF) in biological tissue. We demonstrate coherence-gated SAO TPEF using a transmissive multi-actuator adaptive lens for in vivo imaging in a mouse retina. This configuration has significant potential for reducing the laser power required for adaptive optics multiphoton imaging, and for facilitating integration with existing systems. PMID:27599635

  2. Digital adaptive optics line-scanning confocal imaging system.

    PubMed

    Liu, Changgeng; Kim, Myung K

    2015-01-01

    A digital adaptive optics line-scanning confocal imaging (DAOLCI) system is proposed by applying digital holographic adaptive optics to a digital form of line-scanning confocal imaging system. In DAOLCI, each line scan is recorded by a digital hologram, which allows access to the complex optical field from one slice of the sample through digital holography. This complex optical field contains both the information of one slice of the sample and the optical aberration of the system, thus allowing us to compensate for the effect of the optical aberration, which can be sensed by a complex guide star hologram. After numerical aberration compensation, the corrected optical fields of a sequence of line scans are stitched into the final corrected confocal image. In DAOLCI, a numerical slit is applied to realize the confocality at the sensor end. The width of this slit can be adjusted to control the image contrast and speckle noise for scattering samples. DAOLCI dispenses with the hardware pieces, such as Shack–Hartmann wavefront sensor and deformable mirror, and the closed-loop feedbacks adopted in the conventional adaptive optics confocal imaging system, thus reducing the optomechanical complexity and cost. Numerical simulations and proof-of-principle experiments are presented that demonstrate the feasibility of this idea.

  3. Digital adaptive optics line-scanning confocal imaging system

    PubMed Central

    Liu, Changgeng; Kim, Myung K.

    2015-01-01

    Abstract. A digital adaptive optics line-scanning confocal imaging (DAOLCI) system is proposed by applying digital holographic adaptive optics to a digital form of line-scanning confocal imaging system. In DAOLCI, each line scan is recorded by a digital hologram, which allows access to the complex optical field from one slice of the sample through digital holography. This complex optical field contains both the information of one slice of the sample and the optical aberration of the system, thus allowing us to compensate for the effect of the optical aberration, which can be sensed by a complex guide star hologram. After numerical aberration compensation, the corrected optical fields of a sequence of line scans are stitched into the final corrected confocal image. In DAOLCI, a numerical slit is applied to realize the confocality at the sensor end. The width of this slit can be adjusted to control the image contrast and speckle noise for scattering samples. DAOLCI dispenses with the hardware pieces, such as Shack–Hartmann wavefront sensor and deformable mirror, and the closed-loop feedbacks adopted in the conventional adaptive optics confocal imaging system, thus reducing the optomechanical complexity and cost. Numerical simulations and proof-of-principle experiments are presented that demonstrate the feasibility of this idea. PMID:26140334

  4. Digital adaptive optics line-scanning confocal imaging system.

    PubMed

    Liu, Changgeng; Kim, Myung K

    2015-01-01

    A digital adaptive optics line-scanning confocal imaging (DAOLCI) system is proposed by applying digital holographic adaptive optics to a digital form of line-scanning confocal imaging system. In DAOLCI, each line scan is recorded by a digital hologram, which allows access to the complex optical field from one slice of the sample through digital holography. This complex optical field contains both the information of one slice of the sample and the optical aberration of the system, thus allowing us to compensate for the effect of the optical aberration, which can be sensed by a complex guide star hologram. After numerical aberration compensation, the corrected optical fields of a sequence of line scans are stitched into the final corrected confocal image. In DAOLCI, a numerical slit is applied to realize the confocality at the sensor end. The width of this slit can be adjusted to control the image contrast and speckle noise for scattering samples. DAOLCI dispenses with the hardware pieces, such as Shack–Hartmann wavefront sensor and deformable mirror, and the closed-loop feedbacks adopted in the conventional adaptive optics confocal imaging system, thus reducing the optomechanical complexity and cost. Numerical simulations and proof-of-principle experiments are presented that demonstrate the feasibility of this idea. PMID:26140334

  5. Digital adaptive optics line-scanning confocal imaging system

    NASA Astrophysics Data System (ADS)

    Liu, Changgeng; Kim, Myung K.

    2015-11-01

    A digital adaptive optics line-scanning confocal imaging (DAOLCI) system is proposed by applying digital holographic adaptive optics to a digital form of line-scanning confocal imaging system. In DAOLCI, each line scan is recorded by a digital hologram, which allows access to the complex optical field from one slice of the sample through digital holography. This complex optical field contains both the information of one slice of the sample and the optical aberration of the system, thus allowing us to compensate for the effect of the optical aberration, which can be sensed by a complex guide star hologram. After numerical aberration compensation, the corrected optical fields of a sequence of line scans are stitched into the final corrected confocal image. In DAOLCI, a numerical slit is applied to realize the confocality at the sensor end. The width of this slit can be adjusted to control the image contrast and speckle noise for scattering samples. DAOLCI dispenses with the hardware pieces, such as Shack-Hartmann wavefront sensor and deformable mirror, and the closed-loop feedbacks adopted in the conventional adaptive optics confocal imaging system, thus reducing the optomechanical complexity and cost. Numerical simulations and proof-of-principle experiments are presented that demonstrate the feasibility of this idea.

  6. Adaptive optics technology for high-resolution retinal imaging.

    PubMed

    Lombardo, Marco; Serrao, Sebastiano; Devaney, Nicholas; Parravano, Mariacristina; Lombardo, Giuseppe

    2012-12-27

    Adaptive optics (AO) is a technology used to improve the performance of optical systems by reducing the effects of optical aberrations. The direct visualization of the photoreceptor cells, capillaries and nerve fiber bundles represents the major benefit of adding AO to retinal imaging. Adaptive optics is opening a new frontier for clinical research in ophthalmology, providing new information on the early pathological changes of the retinal microstructures in various retinal diseases. We have reviewed AO technology for retinal imaging, providing information on the core components of an AO retinal camera. The most commonly used wavefront sensing and correcting elements are discussed. Furthermore, we discuss current applications of AO imaging to a population of healthy adults and to the most frequent causes of blindness, including diabetic retinopathy, age-related macular degeneration and glaucoma. We conclude our work with a discussion on future clinical prospects for AO retinal imaging.

  7. Adaptive Optics Technology for High-Resolution Retinal Imaging

    PubMed Central

    Lombardo, Marco; Serrao, Sebastiano; Devaney, Nicholas; Parravano, Mariacristina; Lombardo, Giuseppe

    2013-01-01

    Adaptive optics (AO) is a technology used to improve the performance of optical systems by reducing the effects of optical aberrations. The direct visualization of the photoreceptor cells, capillaries and nerve fiber bundles represents the major benefit of adding AO to retinal imaging. Adaptive optics is opening a new frontier for clinical research in ophthalmology, providing new information on the early pathological changes of the retinal microstructures in various retinal diseases. We have reviewed AO technology for retinal imaging, providing information on the core components of an AO retinal camera. The most commonly used wavefront sensing and correcting elements are discussed. Furthermore, we discuss current applications of AO imaging to a population of healthy adults and to the most frequent causes of blindness, including diabetic retinopathy, age-related macular degeneration and glaucoma. We conclude our work with a discussion on future clinical prospects for AO retinal imaging. PMID:23271600

  8. Contrast-based sensorless adaptive optics for retinal imaging.

    PubMed

    Zhou, Xiaolin; Bedggood, Phillip; Bui, Bang; Nguyen, Christine T O; He, Zheng; Metha, Andrew

    2015-09-01

    Conventional adaptive optics ophthalmoscopes use wavefront sensing methods to characterize ocular aberrations for real-time correction. However, there are important situations in which the wavefront sensing step is susceptible to difficulties that affect the accuracy of the correction. To circumvent these, wavefront sensorless adaptive optics (or non-wavefront sensing AO; NS-AO) imaging has recently been developed and has been applied to point-scanning based retinal imaging modalities. In this study we show, for the first time, contrast-based NS-AO ophthalmoscopy for full-frame in vivo imaging of human and animal eyes. We suggest a robust image quality metric that could be used for any imaging modality, and test its performance against other metrics using (physical) model eyes.

  9. Contrast-based sensorless adaptive optics for retinal imaging

    PubMed Central

    Zhou, Xiaolin; Bedggood, Phillip; Bui, Bang; Nguyen, Christine T.O.; He, Zheng; Metha, Andrew

    2015-01-01

    Conventional adaptive optics ophthalmoscopes use wavefront sensing methods to characterize ocular aberrations for real-time correction. However, there are important situations in which the wavefront sensing step is susceptible to difficulties that affect the accuracy of the correction. To circumvent these, wavefront sensorless adaptive optics (or non-wavefront sensing AO; NS-AO) imaging has recently been developed and has been applied to point-scanning based retinal imaging modalities. In this study we show, for the first time, contrast-based NS-AO ophthalmoscopy for full-frame in vivo imaging of human and animal eyes. We suggest a robust image quality metric that could be used for any imaging modality, and test its performance against other metrics using (physical) model eyes. PMID:26417525

  10. eXtreme Adaptive Optics Planet Imager: Overview and status

    SciTech Connect

    Macintosh, B A; Bauman, B; Evans, J W; Graham, J; Lockwood, C; Poyneer, L; Dillon, D; Gavel, D; Green, J; Lloyd, J; Makidon, R; Olivier, S; Palmer, D; Perrin, M; Severson, S; Sheinis, A; Sivaramakrishnan, A; Sommargren, G; Soumer, R; Troy, M; Wallace, K; Wishnow, E

    2004-08-18

    As adaptive optics (AO) matures, it becomes possible to envision AO systems oriented towards specific important scientific goals rather than general-purpose systems. One such goal for the next decade is the direct imaging detection of extrasolar planets. An 'extreme' adaptive optics (ExAO) system optimized for extrasolar planet detection will have very high actuator counts and rapid update rates - designed for observations of bright stars - and will require exquisite internal calibration at the nanometer level. In addition to extrasolar planet detection, such a system will be capable of characterizing dust disks around young or mature stars, outflows from evolved stars, and high Strehl ratio imaging even at visible wavelengths. The NSF Center for Adaptive Optics has carried out a detailed conceptual design study for such an instrument, dubbed the eXtreme Adaptive Optics Planet Imager or XAOPI. XAOPI is a 4096-actuator AO system, notionally for the Keck telescope, capable of achieving contrast ratios >10{sup 7} at angular separations of 0.2-1'. ExAO system performance analysis is quite different than conventional AO systems - the spatial and temporal frequency content of wavefront error sources is as critical as their magnitude. We present here an overview of the XAOPI project, and an error budget highlighting the key areas determining achievable contrast. The most challenging requirement is for residual static errors to be less than 2 nm over the controlled range of spatial frequencies. If this can be achieved, direct imaging of extrasolar planets will be feasible within this decade.

  11. Limitations to adaptive optics image quality in rodent eyes.

    PubMed

    Zhou, Xiaolin; Bedggood, Phillip; Metha, Andrew

    2012-08-01

    Adaptive optics (AO) retinal image quality of rodent eyes is inferior to that of human eyes, despite the promise of greater numerical aperture. This paradox challenges several assumptions commonly made in AO imaging, assumptions which may be invalidated by the very high power and dioptric thickness of the rodent retina. We used optical modeling to compare the performance of rat and human eyes under conditions that tested the validity of these assumptions. Results showed that AO image quality in the human eye is robust to positioning errors of the AO corrector and to differences in imaging depth and wavelength compared to the wavefront beacon. In contrast, image quality in the rat eye declines sharply with each of these manipulations, especially when imaging off-axis. However, some latitude does exist to offset these manipulations against each other to produce good image quality.

  12. An adaptive optics imaging system designed for clinical use.

    PubMed

    Zhang, Jie; Yang, Qiang; Saito, Kenichi; Nozato, Koji; Williams, David R; Rossi, Ethan A

    2015-06-01

    Here we demonstrate a new imaging system that addresses several major problems limiting the clinical utility of conventional adaptive optics scanning light ophthalmoscopy (AOSLO), including its small field of view (FOV), reliance on patient fixation for targeting imaging, and substantial post-processing time. We previously showed an efficient image based eye tracking method for real-time optical stabilization and image registration in AOSLO. However, in patients with poor fixation, eye motion causes the FOV to drift substantially, causing this approach to fail. We solve that problem here by tracking eye motion at multiple spatial scales simultaneously by optically and electronically integrating a wide FOV SLO (WFSLO) with an AOSLO. This multi-scale approach, implemented with fast tip/tilt mirrors, has a large stabilization range of ± 5.6°. Our method consists of three stages implemented in parallel: 1) coarse optical stabilization driven by a WFSLO image, 2) fine optical stabilization driven by an AOSLO image, and 3) sub-pixel digital registration of the AOSLO image. We evaluated system performance in normal eyes and diseased eyes with poor fixation. Residual image motion with incremental compensation after each stage was: 1) ~2-3 arc minutes, (arcmin) 2) ~0.5-0.8 arcmin and, 3) ~0.05-0.07 arcmin, for normal eyes. Performance in eyes with poor fixation was: 1) ~3-5 arcmin, 2) ~0.7-1.1 arcmin and 3) ~0.07-0.14 arcmin. We demonstrate that this system is capable of reducing image motion by a factor of ~400, on average. This new optical design provides additional benefits for clinical imaging, including a steering subsystem for AOSLO that can be guided by the WFSLO to target specific regions of interest such as retinal pathology and real-time averaging of registered images to eliminate image post-processing.

  13. An adaptive optics imaging system designed for clinical use.

    PubMed

    Zhang, Jie; Yang, Qiang; Saito, Kenichi; Nozato, Koji; Williams, David R; Rossi, Ethan A

    2015-06-01

    Here we demonstrate a new imaging system that addresses several major problems limiting the clinical utility of conventional adaptive optics scanning light ophthalmoscopy (AOSLO), including its small field of view (FOV), reliance on patient fixation for targeting imaging, and substantial post-processing time. We previously showed an efficient image based eye tracking method for real-time optical stabilization and image registration in AOSLO. However, in patients with poor fixation, eye motion causes the FOV to drift substantially, causing this approach to fail. We solve that problem here by tracking eye motion at multiple spatial scales simultaneously by optically and electronically integrating a wide FOV SLO (WFSLO) with an AOSLO. This multi-scale approach, implemented with fast tip/tilt mirrors, has a large stabilization range of ± 5.6°. Our method consists of three stages implemented in parallel: 1) coarse optical stabilization driven by a WFSLO image, 2) fine optical stabilization driven by an AOSLO image, and 3) sub-pixel digital registration of the AOSLO image. We evaluated system performance in normal eyes and diseased eyes with poor fixation. Residual image motion with incremental compensation after each stage was: 1) ~2-3 arc minutes, (arcmin) 2) ~0.5-0.8 arcmin and, 3) ~0.05-0.07 arcmin, for normal eyes. Performance in eyes with poor fixation was: 1) ~3-5 arcmin, 2) ~0.7-1.1 arcmin and 3) ~0.07-0.14 arcmin. We demonstrate that this system is capable of reducing image motion by a factor of ~400, on average. This new optical design provides additional benefits for clinical imaging, including a steering subsystem for AOSLO that can be guided by the WFSLO to target specific regions of interest such as retinal pathology and real-time averaging of registered images to eliminate image post-processing. PMID:26114033

  14. Performance of the Gemini Planet Imager's adaptive optics system.

    PubMed

    Poyneer, Lisa A; Palmer, David W; Macintosh, Bruce; Savransky, Dmitry; Sadakuni, Naru; Thomas, Sandrine; Véran, Jean-Pierre; Follette, Katherine B; Greenbaum, Alexandra Z; Ammons, S Mark; Bailey, Vanessa P; Bauman, Brian; Cardwell, Andrew; Dillon, Daren; Gavel, Donald; Hartung, Markus; Hibon, Pascale; Perrin, Marshall D; Rantakyrö, Fredrik T; Sivaramakrishnan, Anand; Wang, Jason J

    2016-01-10

    The Gemini Planet Imager's adaptive optics (AO) subsystem was designed specifically to facilitate high-contrast imaging. A definitive description of the system's algorithms and technologies as built is given. 564 AO telemetry measurements from the Gemini Planet Imager Exoplanet Survey campaign are analyzed. The modal gain optimizer tracks changes in atmospheric conditions. Science observations show that image quality can be improved with the use of both the spatially filtered wavefront sensor and linear-quadratic-Gaussian control of vibration. The error budget indicates that for all targets and atmospheric conditions AO bandwidth error is the largest term.

  15. New CCD imagers for adaptive optics wavefront sensors

    NASA Astrophysics Data System (ADS)

    Schuette, Daniel R.; Reich, Robert K.; Prigozhin, Ilya; Burke, Barry E.; Johnson, Robert

    2014-08-01

    We report on two recently developed charge-coupled devices (CCDs) for adaptive optics wavefront sensing, both designed to provide exceptional sensitivity (low noise and high quantum efficiency) in high-frame-rate low-latency readout applications. The first imager, the CCID75, is a back-illuminated 16-port 160×160-pixel CCD that has been demonstrated to operate at frame rates above 1,300 fps with noise of < 3 e-. We will describe the architecture of this CCD that enables this level of performance, present and discuss characterization data, and review additional design features that enable unique operating modes for adaptive optics wavefront sensing. We will also present an architectural overview and initial characterization data of a recently designed variation on the CCID75 architecture, the CCID82, which incorporates an electronic shutter to support adaptive optics using Rayleigh beacons.

  16. Focusing a NIR adaptive optics imager; experience with GSAOI

    NASA Astrophysics Data System (ADS)

    Doolan, Matthew; Bloxham, Gabe; Conroy, Peter; Jones, Damien; McGregor, Peter; Stevanovic, Dejan; Van Harmelen, Jan; Waldron, Liam E.; Waterson, Mark; Zhelem, Ross

    2006-06-01

    The Gemini South Adaptive Optics Imager (GSAOI) to be used with the Multi-Conjugate Adaptive Optics (MCAO) system at Gemini South is currently in the final stages of assembly and testing. GSAOI uses a suite of 26 different filters, made from both BK7 and Fused Silica substrates. These filters, located in a non-collimated beam, work as active optical elements. The optical design was undertaken to ensure that both the filter substrates both focused longitudinally at the same point. During the testing of the instrument it was found that longitudinal focus was filter dependant. The methods used to investigate this are outlined in the paper. These investigations identified several possible causes for the focal shift including substrate material properties in cryogenic conditions and small amounts of residual filter power.

  17. Adaptive optics with pupil tracking for high resolution retinal imaging.

    PubMed

    Sahin, Betul; Lamory, Barbara; Levecq, Xavier; Harms, Fabrice; Dainty, Chris

    2012-02-01

    Adaptive optics, when integrated into retinal imaging systems, compensates for rapidly changing ocular aberrations in real time and results in improved high resolution images that reveal the photoreceptor mosaic. Imaging the retina at high resolution has numerous potential medical applications, and yet for the development of commercial products that can be used in the clinic, the complexity and high cost of the present research systems have to be addressed. We present a new method to control the deformable mirror in real time based on pupil tracking measurements which uses the default camera for the alignment of the eye in the retinal imaging system and requires no extra cost or hardware. We also present the first experiments done with a compact adaptive optics flood illumination fundus camera where it was possible to compensate for the higher order aberrations of a moving model eye and in vivo in real time based on pupil tracking measurements, without the real time contribution of a wavefront sensor. As an outcome of this research, we showed that pupil tracking can be effectively used as a low cost and practical adaptive optics tool for high resolution retinal imaging because eye movements constitute an important part of the ocular wavefront dynamics.

  18. Precision Imaging with Adaptive Optics Aperture Masking Interferometry

    NASA Astrophysics Data System (ADS)

    Martinache, F.; Lloyd, J. P.; Tuthill, P.; Woodruff, H. C.; ten Brummelaar, T.; Turner, N.

    2005-12-01

    Adaptive Optics (AO) enables sensitive diffraction limited imaging from the ground on large telescopes. Much of the promise of AO has yet to be fully realised, due to the difficulties imposed by the complicated, unstable and unknown PSF. At the highest resolutions, (inside the PSF) AO has yet to demonstrate full potential for improvements over speckle techniques. The most precise astronomical speckle imaging observations have resulted from non-redundant pupil masking. We are developing a technique to solve the problem of PSF characterization in AO imaging by synthesizing the heritage of image reconstruction with sparse pupil sampling from astronomical interferometry with the long coherence times available after AO correction. Masking the output pupil of the AO system with a non-redundant array can provide self-calibrated imaging. Further calibration of the MTF can be provided with AO wavefront sensor telemetry data. With a precision calibrated PSF, reliable, well-posed deconvolution is possible. High SNR data and accurate MTF calibration provided by the combination of non-redundant masking and AO system telemetry, allow super-resolution. AEOS provides a unique capability to explore the dynamic range and imaging precision of this technique at visible wavelengths. The NSF/AFOSR program has funded an instrument to explore these new imaging techniques at AEOS. ZOR/AO (Zero Optical Redundance with Adaptive Optics) is presently under construction, to be deployed at AEOS in 2005.

  19. Light sheet adaptive optics microscope for 3D live imaging

    NASA Astrophysics Data System (ADS)

    Bourgenot, C.; Taylor, J. M.; Saunter, C. D.; Girkin, J. M.; Love, G. D.

    2013-02-01

    We report on the incorporation of adaptive optics (AO) into the imaging arm of a selective plane illumination microscope (SPIM). SPIM has recently emerged as an important tool for life science research due to its ability to deliver high-speed, optically sectioned, time-lapse microscope images from deep within in vivo selected samples. SPIM provides a very interesting system for the incorporation of AO as the illumination and imaging paths are decoupled and AO may be useful in both paths. In this paper, we will report the use of AO applied to the imaging path of a SPIM, demonstrating significant improvement in image quality of a live GFP-labeled transgenic zebrafish embryo heart using a modal, wavefront sensorless approach and a heart synchronization method. These experimental results are linked to a computational model showing that significant aberrations are produced by the tube holding the sample in addition to the aberration from the biological sample itself.

  20. Imaging of retinal vasculature using adaptive optics SLO/OCT.

    PubMed

    Felberer, Franz; Rechenmacher, Matthias; Haindl, Richard; Baumann, Bernhard; Hitzenberger, Christoph K; Pircher, Michael

    2015-04-01

    We use our previously developed adaptive optics (AO) scanning laser ophthalmoscope (SLO)/ optical coherence tomography (OCT) instrument to investigate its capability for imaging retinal vasculature. The system records SLO and OCT images simultaneously with a pixel to pixel correspondence which allows a direct comparison between those imaging modalities. Different field of views ranging from 0.8°x0.8° up to 4°x4° are supported by the instrument. In addition a dynamic focus scheme was developed for the AO-SLO/OCT system in order to maintain the high transverse resolution throughout imaging depth. The active axial eye tracking that is implemented in the OCT channel allows time resolved measurements of the retinal vasculature in the en-face imaging plane. Vessel walls and structures that we believe correspond to individual erythrocytes could be visualized with the system.

  1. Adapting smartphones for low-cost optical medical imaging

    NASA Astrophysics Data System (ADS)

    Pratavieira, Sebastião.; Vollet-Filho, José D.; Carbinatto, Fernanda M.; Blanco, Kate; Inada, Natalia M.; Bagnato, Vanderlei S.; Kurachi, Cristina

    2015-06-01

    Optical images have been used in several medical situations to improve diagnosis of lesions or to monitor treatments. However, most systems employ expensive scientific (CCD or CMOS) cameras and need computers to display and save the images, usually resulting in a high final cost for the system. Additionally, this sort of apparatus operation usually becomes more complex, requiring more and more specialized technical knowledge from the operator. Currently, the number of people using smartphone-like devices with built-in high quality cameras is increasing, which might allow using such devices as an efficient, lower cost, portable imaging system for medical applications. Thus, we aim to develop methods of adaptation of those devices to optical medical imaging techniques, such as fluorescence. Particularly, smartphones covers were adapted to connect a smartphone-like device to widefield fluorescence imaging systems. These systems were used to detect lesions in different tissues, such as cervix and mouth/throat mucosa, and to monitor ALA-induced protoporphyrin-IX formation for photodynamic treatment of Cervical Intraepithelial Neoplasia. This approach may contribute significantly to low-cost, portable and simple clinical optical imaging collection.

  2. Widefield multiphoton microscopy with image-based adaptive optics

    NASA Astrophysics Data System (ADS)

    Chang, C.-Y.; Cheng, L.-C.; Su, H.-W.; Yen, W.-C.; Chen, S.-J.

    2012-10-01

    Unlike conventional multiphoton microscopy according to pixel by pixel point scanning, a widefield multiphoton microscope based on spatiotemporal focusing has been developed to provide fast optical sectioning images at a frame rate over 100 Hz. In order to overcome the aberrations of the widefield multiphoton microscope and the wavefront distortion from turbid biospecimens, an image-based adaptive optics system (AOS) was integrated. The feedback control signal of the AOS was acquired according to locally maximize image intensity, which were provided by the widefield multiphoton excited microscope, by using a hill climbing algorithm. Then, the control signal was utilized to drive a deformable mirror in such a way as to eliminate the aberration and distortion. A R6G-doped PMMA thin film is also increased by 3.7-fold. Furthermore, the TPEF image quality of 1 μm fluorescent beads sealed in agarose gel at different depths is improved.

  3. Objective assessment of image quality. IV. Application to adaptive optics

    PubMed Central

    Barrett, Harrison H.; Myers, Kyle J.; Devaney, Nicholas; Dainty, Christopher

    2008-01-01

    The methodology of objective assessment, which defines image quality in terms of the performance of specific observers on specific tasks of interest, is extended to temporal sequences of images with random point spread functions and applied to adaptive imaging in astronomy. The tasks considered include both detection and estimation, and the observers are the optimal linear discriminant (Hotelling observer) and the optimal linear estimator (Wiener). A general theory of first- and second-order spatiotemporal statistics in adaptive optics is developed. It is shown that the covariance matrix can be rigorously decomposed into three terms representing the effect of measurement noise, random point spread function, and random nature of the astronomical scene. Figures of merit are developed, and computational methods are discussed. PMID:17106464

  4. Objective assessment of image quality. IV. Application to adaptive optics.

    PubMed

    Barrett, Harrison H; Myers, Kyle J; Devaney, Nicholas; Dainty, Christopher

    2006-12-01

    The methodology of objective assessment, which defines image quality in terms of the performance of specific observers on specific tasks of interest, is extended to temporal sequences of images with random point spread functions and applied to adaptive imaging in astronomy. The tasks considered include both detection and estimation, and the observers are the optimal linear discriminant (Hotelling observer) and the optimal linear estimator (Wiener). A general theory of first- and second-order spatiotemporal statistics in adaptive optics is developed. It is shown that the covariance matrix can be rigorously decomposed into three terms representing the effect of measurement noise, random point spread function, and random nature of the astronomical scene. Figures of merit are developed, and computational methods are discussed.

  5. Objective assessment of image quality. IV. Application to adaptive optics.

    PubMed

    Barrett, Harrison H; Myers, Kyle J; Devaney, Nicholas; Dainty, Christopher

    2006-12-01

    The methodology of objective assessment, which defines image quality in terms of the performance of specific observers on specific tasks of interest, is extended to temporal sequences of images with random point spread functions and applied to adaptive imaging in astronomy. The tasks considered include both detection and estimation, and the observers are the optimal linear discriminant (Hotelling observer) and the optimal linear estimator (Wiener). A general theory of first- and second-order spatiotemporal statistics in adaptive optics is developed. It is shown that the covariance matrix can be rigorously decomposed into three terms representing the effect of measurement noise, random point spread function, and random nature of the astronomical scene. Figures of merit are developed, and computational methods are discussed. PMID:17106464

  6. Image-quality metrics for characterizing adaptive optics system performance.

    PubMed

    Brigantic, R T; Roggemann, M C; Bauer, K W; Welsh, B M

    1997-09-10

    Adaptive optics system (AOS) performance is a function of the system design, seeing conditions, and light level of the wave-front beacon. It is desirable to optimize the controllable parameters in an AOS to maximize some measure of performance. For this optimization to be useful, it is necessary that a set of image-quality metrics be developed that vary monotonically with the AOS performance under a wide variety of imaging environments. Accordingly, as conditions change, one can be confident that the computed metrics dictate appropriate system settings that will optimize performance. Three such candidate metrics are presented. The first is the Strehl ratio; the second is a novel metric that modifies the Strehl ratio by integration of the modulus of the average system optical transfer function to a noise-effective cutoff frequency at which some specified image spectrum signal-to-noise ratio level is attained; and the third is simply the cutoff frequency just mentioned. It is shown that all three metrics are correlated with the rms error (RMSE) between the measured image and the associated diffraction-limited image. Of these, the Strehl ratio and the modified Strehl ratio exhibit consistently high correlations with the RMSE across a broad range of conditions and system settings. Furthermore, under conditions that yield a constant average system optical transfer function, the modified Strehl ratio can still be used to delineate image quality, whereas the Strehl ratio cannot.

  7. Accuracy requirements of optical linear algebra processors in adaptive optics imaging systems.

    PubMed

    Downie, J D; Goodman, J W

    1989-10-15

    A ground-based adaptive optics imaging telescope system attempts to improve image quality by measuring and correcting for atmospherically induced wavefront aberrations. The necessary control computations during each cycle will take a finite amount of time, which adds to the residual error variance since the atmosphere continues to change during that time. Thus an optical processor may be well-suited for this task. This paper investigates this possibility by studying the accuracy requirements in a general optical processor that will make it competitive with, or superior to, a conventional digital computer for adaptive optics use.

  8. Fourier transform digital holographic adaptive optics imaging system

    PubMed Central

    Liu, Changgeng; Yu, Xiao; Kim, Myung K.

    2013-01-01

    A Fourier transform digital holographic adaptive optics imaging system and its basic principles are proposed. The CCD is put at the exact Fourier transform plane of the pupil of the eye lens. The spherical curvature introduced by the optics except the eye lens itself is eliminated. The CCD is also at image plane of the target. The point-spread function of the system is directly recorded, making it easier to determine the correct guide-star hologram. Also, the light signal will be stronger at the CCD, especially for phase-aberration sensing. Numerical propagation is avoided. The sensor aperture has nothing to do with the resolution and the possibility of using low coherence or incoherent illumination is opened. The system becomes more efficient and flexible. Although it is intended for ophthalmic use, it also shows potential application in microscopy. The robustness and feasibility of this compact system are demonstrated by simulations and experiments using scattering objects. PMID:23262541

  9. Accuracy requirements of optical linear algebra processors in adaptive optics imaging systems

    NASA Technical Reports Server (NTRS)

    Downie, John D.

    1990-01-01

    A ground-based adaptive optics imaging telescope system attempts to improve image quality by detecting and correcting for atmospherically induced wavefront aberrations. The required control computations during each cycle will take a finite amount of time. Longer time delays result in larger values of residual wavefront error variance since the atmosphere continues to change during that time. Thus an optical processor may be well-suited for this task. This paper presents a study of the accuracy requirements in a general optical processor that will make it competitive with, or superior to, a conventional digital computer for the adaptive optics application. An optimization of the adaptive optics correction algorithm with respect to an optical processor's degree of accuracy is also briefly discussed.

  10. EUV imaging experiment of an adaptive optics telescope

    NASA Astrophysics Data System (ADS)

    Kitamoto, S.; Shibata, T.; Takenaka, E.; Yoshida, M.; Murakami, H.; Shishido, Y.; Gotoh, N.; Nagasaki, K.; Takei, D.; Morii, M.

    2009-08-01

    We report an experimental result of our normal-incident EUV telescope tuned to a 13.5 nm band, with an adaptive optics. The optics consists of a spherical primary mirror and a secondary mirror. Both are coated by Mo/Si multilayer. The diameter of the primary and the secondary mirrors are 80 mm and 55mm, respectively. The secondary mirror is a deformable mirror with 31 bimorph-piezo electrodes. The EUV from a laser plasma source was exposed to a Ni mesh with 31 micro-m wires. The image of this mesh was obtained by a backilluminated CCD. The reference wave was made by an optical laser source with 1 μm pin-hole. We measure the wave form of this reference wave and control the secondary mirror to get a good EUV image. Since the paths of EUV and the optical light for the reference were different from each other, we modify the target wave from to control the deformable mirror, as the EUV image is best. The higher order Zernike components of the target wave form, as well as the tilts and focus components, were added to the reference wave form made by simply calculated. We confirmed the validity of this control and performed a 2.1 arc-sec resolution.

  11. Adaptive Optics and Lucky Imager (AOLI): presentation and first light

    NASA Astrophysics Data System (ADS)

    Velasco, S.; Rebolo, R.; Mackay, C.; Oscoz, A.; King, D. L.; Crass, J.; Díaz-Sánchez, A.; Femenía, B.; González-Escalera, V.; Labadie, L.; López, R. L.; Pérez Garrido, A.; Puga, M.; Rodríguez-Ramos, L. F.; Zuther, J.

    2015-05-01

    In this paper we present the Adaptive Optics Lucky Imager (AOLI), a state-of-the-art instrument which makes use of two well proved techniques for extremely high spatial resolution with ground-based telescopes: Lucky Imaging (LI) and Adaptive Optics (AO). AOLI comprises an AO system, including a low order non-linear curvature wavefront sensor together with a 241 actuators deformable mirror, a science array of four 1024x1024 EMCCDs, allowing a 120×120 down to 36×36" field of view, a calibration subsystem and a powerful LI software. Thanks to the revolutionary WFS, AOLI shall have the capability of using faint reference stars (I˜16.5-17.5), enabling it to be used over a much wider part of the sky than with common Shack-Hartmann AO systems. This instrument saw first light in September 2013 at William Herschel Telescope. Although the instrument was not complete, these commissioning demonstrated its feasibility, obtaining a FWHM for the best PSF of 0.151±0.005" and a plate scale of 55.0±0.3 {mas} {pix}^{-1}. Those observations served us to prove some characteristics of the interesting multiple T Tauri system LkHα 262-263, finding it to be gravitationally bounded. This interesting multiple system mixes the presence of proto-planetary discs, one proved to be double, and the first-time optically resolved pair LkHα 263AB (0.42" separation).

  12. Adaptive optics scanning laser ophthalmoscope imaging: technology update.

    PubMed

    Merino, David; Loza-Alvarez, Pablo

    2016-01-01

    Adaptive optics (AO) retinal imaging has become very popular in the past few years, especially within the ophthalmic research community. Several different retinal techniques, such as fundus imaging cameras or optical coherence tomography systems, have been coupled with AO in order to produce impressive images showing individual cell mosaics over different layers of the in vivo human retina. The combination of AO with scanning laser ophthalmoscopy has been extensively used to generate impressive images of the human retina with unprecedented resolution, showing individual photoreceptor cells, retinal pigment epithelium cells, as well as microscopic capillary vessels, or the nerve fiber layer. Over the past few years, the technique has evolved to develop several different applications not only in the clinic but also in different animal models, thanks to technological developments in the field. These developments have specific applications to different fields of investigation, which are not limited to the study of retinal diseases but also to the understanding of the retinal function and vision science. This review is an attempt to summarize these developments in an understandable and brief manner in order to guide the reader into the possibilities that AO scanning laser ophthalmoscopy offers, as well as its limitations, which should be taken into account when planning on using it.

  13. Adaptive optics scanning laser ophthalmoscope imaging: technology update

    PubMed Central

    Merino, David; Loza-Alvarez, Pablo

    2016-01-01

    Adaptive optics (AO) retinal imaging has become very popular in the past few years, especially within the ophthalmic research community. Several different retinal techniques, such as fundus imaging cameras or optical coherence tomography systems, have been coupled with AO in order to produce impressive images showing individual cell mosaics over different layers of the in vivo human retina. The combination of AO with scanning laser ophthalmoscopy has been extensively used to generate impressive images of the human retina with unprecedented resolution, showing individual photoreceptor cells, retinal pigment epithelium cells, as well as microscopic capillary vessels, or the nerve fiber layer. Over the past few years, the technique has evolved to develop several different applications not only in the clinic but also in different animal models, thanks to technological developments in the field. These developments have specific applications to different fields of investigation, which are not limited to the study of retinal diseases but also to the understanding of the retinal function and vision science. This review is an attempt to summarize these developments in an understandable and brief manner in order to guide the reader into the possibilities that AO scanning laser ophthalmoscopy offers, as well as its limitations, which should be taken into account when planning on using it. PMID:27175057

  14. Adaptive Optics Imaging Survey of Luminous Infrared Galaxies

    SciTech Connect

    Laag, E A; Canalizo, G; van Breugel, W; Gates, E L; de Vries, W; Stanford, S A

    2006-03-13

    We present high resolution imaging observations of a sample of previously unidentified far-infrared galaxies at z < 0.3. The objects were selected by cross-correlating the IRAS Faint Source Catalog with the VLA FIRST catalog and the HST Guide Star Catalog to allow for adaptive optics observations. We found two new ULIGs (with L{sub FIR} {ge} 10{sup 12} L{sub {circle_dot}}) and 19 new LIGs (with L{sub FIR} {ge} 10{sup 11} L{sub {circle_dot}}). Twenty of the galaxies in the sample were imaged with either the Lick or Keck adaptive optics systems in H or K{prime}. Galaxy morphologies were determined using the two dimensional fitting program GALFIT and the residuals examined to look for interesting structure. The morphologies reveal that at least 30% are involved in tidal interactions, with 20% being clear mergers. An additional 50% show signs of possible interaction. Line ratios were used to determine powering mechanism; of the 17 objects in the sample showing clear emission lines--four are active galactic nuclei and seven are starburst galaxies. The rest exhibit a combination of both phenomena.

  15. In vivo imaging of human photoreceptor mosaic with wavefront sensorless adaptive optics optical coherence tomography.

    PubMed

    Wong, Kevin S K; Jian, Yifan; Cua, Michelle; Bonora, Stefano; Zawadzki, Robert J; Sarunic, Marinko V

    2015-02-01

    Wavefront sensorless adaptive optics optical coherence tomography (WSAO-OCT) is a novel imaging technique for in vivo high-resolution depth-resolved imaging that mitigates some of the challenges encountered with the use of sensor-based adaptive optics designs. This technique replaces the Hartmann Shack wavefront sensor used to measure aberrations with a depth-resolved image-driven optimization algorithm, with the metric based on the OCT volumes acquired in real-time. The custom-built ultrahigh-speed GPU processing platform and fast modal optimization algorithm presented in this paper was essential in enabling real-time, in vivo imaging of human retinas with wavefront sensorless AO correction. WSAO-OCT is especially advantageous for developing a clinical high-resolution retinal imaging system as it enables the use of a compact, low-cost and robust lens-based adaptive optics design. In this report, we describe our WSAO-OCT system for imaging the human photoreceptor mosaic in vivo. We validated our system performance by imaging the retina at several eccentricities, and demonstrated the improvement in photoreceptor visibility with WSAO compensation.

  16. In vivo imaging of human photoreceptor mosaic with wavefront sensorless adaptive optics optical coherence tomography

    PubMed Central

    Wong, Kevin S. K.; Jian, Yifan; Cua, Michelle; Bonora, Stefano; Zawadzki, Robert J.; Sarunic, Marinko V.

    2015-01-01

    Wavefront sensorless adaptive optics optical coherence tomography (WSAO-OCT) is a novel imaging technique for in vivo high-resolution depth-resolved imaging that mitigates some of the challenges encountered with the use of sensor-based adaptive optics designs. This technique replaces the Hartmann Shack wavefront sensor used to measure aberrations with a depth-resolved image-driven optimization algorithm, with the metric based on the OCT volumes acquired in real-time. The custom-built ultrahigh-speed GPU processing platform and fast modal optimization algorithm presented in this paper was essential in enabling real-time, in vivo imaging of human retinas with wavefront sensorless AO correction. WSAO-OCT is especially advantageous for developing a clinical high-resolution retinal imaging system as it enables the use of a compact, low-cost and robust lens-based adaptive optics design. In this report, we describe our WSAO-OCT system for imaging the human photoreceptor mosaic in vivo. We validated our system performance by imaging the retina at several eccentricities, and demonstrated the improvement in photoreceptor visibility with WSAO compensation. PMID:25780747

  17. High-resolution retinal imaging using adaptive optics and Fourier-domain optical coherence tomography

    DOEpatents

    Olivier, Scot S.; Werner, John S.; Zawadzki, Robert J.; Laut, Sophie P.; Jones, Steven M.

    2010-09-07

    This invention permits retinal images to be acquired at high speed and with unprecedented resolution in three dimensions (4.times.4.times.6 .mu.m). The instrument achieves high lateral resolution by using adaptive optics to correct optical aberrations of the human eye in real time. High axial resolution and high speed are made possible by the use of Fourier-domain optical coherence tomography. Using this system, we have demonstrated the ability to image microscopic blood vessels and the cone photoreceptor mosaic.

  18. Adaptive optics retinal imaging in the living mouse eye.

    PubMed

    Geng, Ying; Dubra, Alfredo; Yin, Lu; Merigan, William H; Sharma, Robin; Libby, Richard T; Williams, David R

    2012-04-01

    Correction of the eye's monochromatic aberrations using adaptive optics (AO) can improve the resolution of in vivo mouse retinal images [Biss et al., Opt. Lett. 32(6), 659 (2007) and Alt et al., Proc. SPIE 7550, 755019 (2010)], but previous attempts have been limited by poor spot quality in the Shack-Hartmann wavefront sensor (SHWS). Recent advances in mouse eye wavefront sensing using an adjustable focus beacon with an annular beam profile have improved the wavefront sensor spot quality [Geng et al., Biomed. Opt. Express 2(4), 717 (2011)], and we have incorporated them into a fluorescence adaptive optics scanning laser ophthalmoscope (AOSLO). The performance of the instrument was tested on the living mouse eye, and images of multiple retinal structures, including the photoreceptor mosaic, nerve fiber bundles, fine capillaries and fluorescently labeled ganglion cells were obtained. The in vivo transverse and axial resolutions of the fluorescence channel of the AOSLO were estimated from the full width half maximum (FWHM) of the line and point spread functions (LSF and PSF), and were found to be better than 0.79 μm ± 0.03 μm (STD)(45% wider than the diffraction limit) and 10.8 μm ± 0.7 μm (STD)(two times the diffraction limit), respectively. The axial positional accuracy was estimated to be 0.36 μm. This resolution and positional accuracy has allowed us to classify many ganglion cell types, such as bistratified ganglion cells, in vivo.

  19. Adaptive optics retinal imaging in the living mouse eye

    PubMed Central

    Geng, Ying; Dubra, Alfredo; Yin, Lu; Merigan, William H.; Sharma, Robin; Libby, Richard T.; Williams, David R.

    2012-01-01

    Correction of the eye’s monochromatic aberrations using adaptive optics (AO) can improve the resolution of in vivo mouse retinal images [Biss et al., Opt. Lett. 32(6), 659 (2007) and Alt et al., Proc. SPIE 7550, 755019 (2010)], but previous attempts have been limited by poor spot quality in the Shack-Hartmann wavefront sensor (SHWS). Recent advances in mouse eye wavefront sensing using an adjustable focus beacon with an annular beam profile have improved the wavefront sensor spot quality [Geng et al., Biomed. Opt. Express 2(4), 717 (2011)], and we have incorporated them into a fluorescence adaptive optics scanning laser ophthalmoscope (AOSLO). The performance of the instrument was tested on the living mouse eye, and images of multiple retinal structures, including the photoreceptor mosaic, nerve fiber bundles, fine capillaries and fluorescently labeled ganglion cells were obtained. The in vivo transverse and axial resolutions of the fluorescence channel of the AOSLO were estimated from the full width half maximum (FWHM) of the line and point spread functions (LSF and PSF), and were found to be better than 0.79 μm ± 0.03 μm (STD)(45% wider than the diffraction limit) and 10.8 μm ± 0.7 μm (STD)(two times the diffraction limit), respectively. The axial positional accuracy was estimated to be 0.36 μm. This resolution and positional accuracy has allowed us to classify many ganglion cell types, such as bistratified ganglion cells, in vivo. PMID:22574260

  20. Perceived Image Quality Improvements from the Application of Image Deconvolution to Retinal Images from an Adaptive Optics Fundus Imager

    NASA Astrophysics Data System (ADS)

    Soliz, P.; Nemeth, S. C.; Erry, G. R. G.; Otten, L. J.; Yang, S. Y.

    Aim: The objective of this project was to apply an image restoration methodology based on wavefront measurements obtained with a Shack-Hartmann sensor and evaluating the restored image quality based on medical criteria.Methods: Implementing an adaptive optics (AO) technique, a fundus imager was used to achieve low-order correction to images of the retina. The high-order correction was provided by deconvolution. A Shack-Hartmann wavefront sensor measures aberrations. The wavefront measurement is the basis for activating a deformable mirror. Image restoration to remove remaining aberrations is achieved by direct deconvolution using the point spread function (PSF) or a blind deconvolution. The PSF is estimated using measured wavefront aberrations. Direct application of classical deconvolution methods such as inverse filtering, Wiener filtering or iterative blind deconvolution (IBD) to the AO retinal images obtained from the adaptive optical imaging system is not satisfactory because of the very large image size, dificulty in modeling the system noise, and inaccuracy in PSF estimation. Our approach combines direct and blind deconvolution to exploit available system information, avoid non-convergence, and time-consuming iterative processes. Results: The deconvolution was applied to human subject data and resulting restored images compared by a trained ophthalmic researcher. Qualitative analysis showed significant improvements. Neovascularization can be visualized with the adaptive optics device that cannot be resolved with the standard fundus camera. The individual nerve fiber bundles are easily resolved as are melanin structures in the choroid. Conclusion: This project demonstrated that computer-enhanced, adaptive optic images have greater detail of anatomical and pathological structures.

  1. Adaptive Optics for Satellite Imaging and Space Debris Ranging

    NASA Astrophysics Data System (ADS)

    Bennet, F.; D'Orgeville, C.; Price, I.; Rigaut, F.; Ritchie, I.; Smith, C.

    Earth's space environment is becoming crowded and at risk of a Kessler syndrome, and will require careful management for the future. Modern low noise high speed detectors allow for wavefront sensing and adaptive optics (AO) in extreme circumstances such as imaging small orbiting bodies in Low Earth Orbit (LEO). The Research School of Astronomy and Astrophysics (RSAA) at the Australian National University have been developing AO systems for telescopes between 1 and 2.5m diameter to image and range orbiting satellites and space debris. Strehl ratios in excess of 30% can be achieved for targets in LEO with an AO loop running at 2kHz, allowing the resolution of small features (<30cm) and the capability to determine object shape and spin characteristics. The AO system developed at RSAA consists of a high speed EMCCD Shack-Hartmann wavefront sensor, a deformable mirror (DM), and realtime computer (RTC), and an imaging camera. The system works best as a laser guide star system but will also function as a natural guide star AO system, with the target itself being the guide star. In both circumstances tip-tilt is provided by the target on the imaging camera. The fast tip-tilt modes are not corrected optically, and are instead removed by taking images at a moderate speed (>30Hz) and using a shift and add algorithm. This algorithm can also incorporate lucky imaging to further improve the final image quality. A similar AO system for space debris ranging is also in development in collaboration with Electro Optic Systems (EOS) and the Space Environment Management Cooperative Research Centre (SERC), at the Mount Stromlo Observatory in Canberra, Australia. The system is designed for an AO corrected upward propagated 1064nm pulsed laser beam, from which time of flight information is used to precisely range the target. A 1.8m telescope is used for both propagation and collection of laser light. A laser guide star, Shack-Hartmann wavefront sensor, and DM are used for high order

  2. Coherence gated wavefront sensorless adaptive optics for two photon excited fluorescence retinal imaging (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Jian, Yifan; Cua, Michelle; Bonora, Stefano; Pugh, Edward N.; Zawadzki, Robert J.; Sarunic, Marinko V.

    2016-03-01

    We present a novel system for adaptive optics two photon imaging. We utilize the bandwidth of the femtosecond excitation beam to perform coherence gated imaging (OCT) of the sample. The location of the focus is directly observable in the cross sectional OCT images, and adjusted to the desired depth plane. Next, using real time volumetric OCT, we perform Wavefront Sensorless Adaptive Optics (WSAO) aberration correction using a multi-element adaptive lens capable of correcting up to 4th order Zernike polynomials. The aberration correction is performed based on an image quality metric, for example intensity. The optimization time is limited only by the OCT acquisition rate, and takes ~30s. Following aberration correction, two photon fluorescence images are acquired, and compared to results without adaptive optics correction. This technique is promising for multiphoton imaging in multi-layered, scattering samples such as eye and brain, in which traditional wavefront sensing and guide-star sensorless adaptive optics approaches may not be suitable.

  3. Closed-loop adaptive optics using a CMOS image quality metric sensor

    NASA Astrophysics Data System (ADS)

    Ting, Chueh; Rayankula, Aditya; Giles, Michael K.; Furth, Paul M.

    2006-08-01

    When compared to a Shack-Hartmann sensor, a CMOS image sharpness sensor has the advantage of reduced complexity in a closed-loop adaptive optics system. It also has the potential to be implemented as a smart sensor using VLSI technology. In this paper, we present a novel adaptive optics testbed that uses a CMOS sharpness imager built in the New Mexico State University (NMSU) Electro-Optics Research Laboratory (EORL). The adaptive optics testbed, which includes a CMOS image quality metric sensor and a 37-channel deformable mirror, has the capability to rapidly compensate higher-order phase aberrations. An experimental performance comparison of the pinhole image sharpness feedback method and the CMOS imager is presented. The experimental data shows that the CMOS sharpness imager works well in a closed-loop adaptive optics system. Its overall performance is better than that of the pinhole method, and it has a fast response time.

  4. Adaptive optics images. III. 87 Kepler objects of interest

    SciTech Connect

    Dressing, Courtney D.; Dupree, Andrea K.; Adams, Elisabeth R.; Kulesa, Craig; McCarthy, Don

    2014-11-01

    The Kepler mission has revolutionized our understanding of exoplanets, but some of the planet candidates identified by Kepler may actually be astrophysical false positives or planets whose transit depths are diluted by the presence of another star. Adaptive optics images made with ARIES at the MMT of 87 Kepler Objects of Interest place limits on the presence of fainter stars in or near the Kepler aperture. We detected visual companions within 1'' for 5 stars, between 1'' and 2'' for 7 stars, and between 2'' and 4'' for 15 stars. For those systems, we estimate the brightness of companion stars in the Kepler bandpass and provide approximate corrections to the radii of associated planet candidates due to the extra light in the aperture. For all stars observed, we report detection limits on the presence of nearby stars. ARIES is typically sensitive to stars approximately 5.3 Ks magnitudes fainter than the target star within 1'' and approximately 5.7 Ks magnitudes fainter within 2'', but can detect stars as faint as ΔKs = 7.5 under ideal conditions.

  5. Closed-loop optical stabilization and digital image registration in adaptive optics scanning light ophthalmoscopy.

    PubMed

    Yang, Qiang; Zhang, Jie; Nozato, Koji; Saito, Kenichi; Williams, David R; Roorda, Austin; Rossi, Ethan A

    2014-09-01

    Eye motion is a major impediment to the efficient acquisition of high resolution retinal images with the adaptive optics (AO) scanning light ophthalmoscope (AOSLO). Here we demonstrate a solution to this problem by implementing both optical stabilization and digital image registration in an AOSLO. We replaced the slow scanning mirror with a two-axis tip/tilt mirror for the dual functions of slow scanning and optical stabilization. Closed-loop optical stabilization reduced the amplitude of eye-movement related-image motion by a factor of 10-15. The residual RMS error after optical stabilization alone was on the order of the size of foveal cones: ~1.66-2.56 μm or ~0.34-0.53 arcmin with typical fixational eye motion for normal observers. The full implementation, with real-time digital image registration, corrected the residual eye motion after optical stabilization with an accuracy of ~0.20-0.25 μm or ~0.04-0.05 arcmin RMS, which to our knowledge is more accurate than any method previously reported.

  6. Closed-loop optical stabilization and digital image registration in adaptive optics scanning light ophthalmoscopy

    PubMed Central

    Yang, Qiang; Zhang, Jie; Nozato, Koji; Saito, Kenichi; Williams, David R.; Roorda, Austin; Rossi, Ethan A.

    2014-01-01

    Eye motion is a major impediment to the efficient acquisition of high resolution retinal images with the adaptive optics (AO) scanning light ophthalmoscope (AOSLO). Here we demonstrate a solution to this problem by implementing both optical stabilization and digital image registration in an AOSLO. We replaced the slow scanning mirror with a two-axis tip/tilt mirror for the dual functions of slow scanning and optical stabilization. Closed-loop optical stabilization reduced the amplitude of eye-movement related-image motion by a factor of 10–15. The residual RMS error after optical stabilization alone was on the order of the size of foveal cones: ~1.66–2.56 μm or ~0.34–0.53 arcmin with typical fixational eye motion for normal observers. The full implementation, with real-time digital image registration, corrected the residual eye motion after optical stabilization with an accuracy of ~0.20–0.25 μm or ~0.04–0.05 arcmin RMS, which to our knowledge is more accurate than any method previously reported. PMID:25401030

  7. Frame selection performance limits for statistical image reconstruction of adaptive optics compensated images

    NASA Astrophysics Data System (ADS)

    Ford, Stephen D.

    1994-12-01

    The U.S. Air Force uses adaptive optics systems to collect images of extended objects beyond the atmosphere. These systems use wavefront sensors and deformable mirrors to compensate for atmospheric turbulence induced aberrations. Adaptive optics greatly enhance image quality, however, wavefront aberrations are not completely eliminated. Therefore, post-detection processing techniques are employed to further improve the compensated images. Typically, many short exposure images are collected, recentered to compensate for tilt, and then averaged to overcome randomness in the images and improve signal-to-noise ratio. Experience shows that some short exposure images in a data set are better than others. Frame selection exploits this fact by using a quality metric to discard low quality frames. A composite image is then created by averaging only the best frames. Performance limits associated with the frame selection technique are investigated in this thesis. Limits imposed by photon noise result in a minimum object brightness of visual magnitude +8 for point sources and +4 for a typical satellite model. Effective average point spread functions for point source and extended objects after frame selection processing are almost identical across a wide range of conditions. This discovery allows the use of deconvolution techniques to sharpen images after using the frame selection technique. A new post-detection processing method, frame weighting, is investigated and may offer some improvement for dim objects during poor atmospheric seeing. Frame selection is demonstrated for the first time on actual imagery from an adaptive optics system. Data analysis indicates that signal-to-noise ratio improvements are degraded for exposure times longer than that allowed to 'freeze' individual realizations of the turbulence effects.

  8. Adaptive optics OCT using 1060nm swept source and dual deformable lenses for human retinal imaging

    NASA Astrophysics Data System (ADS)

    Jian, Yifan; Lee, Sujin; Cua, Michelle; Miao, Dongkai; Bonora, Stefano; Zawadzki, Robert J.; Sarunic, Marinko V.

    2016-03-01

    Adaptive optics concepts have been applied to the advancement of biological imaging and microscopy. In particular, AO has also been very successfully applied to cellular resolution imaging of the retina, enabling visualization of the characteristic mosaic patterns of the outer retinal layers using flood illumination fundus photography, Scanning Laser Ophthalmoscopy (SLO), and Optical Coherence Tomography (OCT). Despite the high quality of the in vivo images, there has been a limited uptake of AO imaging into the clinical environment. The high resolution afforded by AO comes at the price of limited field of view and specialized equipment. The implementation of a typical adaptive optics imaging system results in a relatively large and complex optical setup. The wavefront measurement is commonly performed using a Hartmann-Shack Wavefront Sensor (HS-WFS) placed at an image plane that is optically conjugated to the eye's pupil. The deformable mirror is also placed at a conjugate plane, relaying the wavefront corrections to the pupil. Due to the sensitivity of the HS-WFS to back-reflections, the imaging system is commonly constructed from spherical mirrors. In this project, we present a novel adaptive optics OCT retinal imaging system with significant potential to overcome many of the barriers to integration with a clinical environment. We describe in detail the implementation of a compact lens based wavefront sensorless adaptive optics (WSAO) 1060nm swept source OCT human retinal imaging system with dual deformable lenses, and present retinal images acquired in vivo from research volunteers.

  9. Quality evaluation of adaptive optical image based on DCT and Rényi entropy

    NASA Astrophysics Data System (ADS)

    Xu, Yuannan; Li, Junwei; Wang, Jing; Deng, Rong; Dong, Yanbing

    2015-04-01

    The adaptive optical telescopes play a more and more important role in the detection system on the ground, and the adaptive optical images are so many that we need find a suitable method of quality evaluation to choose good quality images automatically in order to save human power. It is well known that the adaptive optical images are no-reference images. In this paper, a new logarithmic evaluation method based on the use of the discrete cosine transform(DCT) and Rényi entropy for the adaptive optical images is proposed. Through the DCT using one or two dimension window, the statistical property of Rényi entropy for images is studied. The different directional Rényi entropy maps of an input image containing different information content are obtained. The mean values of different directional Rényi entropy maps are calculated. For image quality evaluation, the different directional Rényi entropy and its standard deviation corresponding to region of interest is selected as an indicator for the anisotropy of the images. The standard deviation of different directional Rényi entropy is obtained as the quality evaluation value for adaptive optical image. Experimental results show the proposed method that the sorting quality matches well with the visual inspection.

  10. Application of adaptive optics in retinal imaging: a quantitative and clinical comparison with standard cameras

    NASA Astrophysics Data System (ADS)

    Barriga, E. S.; Erry, G.; Yang, S.; Russell, S.; Raman, B.; Soliz, P.

    2005-04-01

    Aim: The objective of this project was to evaluate high resolution images from an adaptive optics retinal imager through comparisons with standard film-based and standard digital fundus imagers. Methods: A clinical prototype adaptive optics fundus imager (AOFI) was used to collect retinal images from subjects with various forms of retinopathy to determine whether improved visibility into the disease could be provided to the clinician. The AOFI achieves low-order correction of aberrations through a closed-loop wavefront sensor and an adaptive optics system. The remaining high-order aberrations are removed by direct deconvolution using the point spread function (PSF) or by blind deconvolution when the PSF is not available. An ophthalmologist compared the AOFI images with standard fundus images and provided a clinical evaluation of all the modalities and processing techniques. All images were also analyzed using a quantitative image quality index. Results: This system has been tested on three human subjects (one normal and two with retinopathy). In the diabetic patient vascular abnormalities were detected with the AOFI that cannot be resolved with the standard fundus camera. Very small features, such as the fine vascular structures on the optic disc and the individual nerve fiber bundles are easily resolved by the AOFI. Conclusion: This project demonstrated that adaptive optic images have great potential in providing clinically significant detail of anatomical and pathological structures to the ophthalmologist.

  11. AVES-IMCO: an adaptive optics visible spectrograph and imager/coronograph for NAOS

    NASA Astrophysics Data System (ADS)

    Beuzit, Jean-Luc; Lagrange, A.-M.; Mouillet, D.; Chauvin, G.; Stadler, E.; Charton, J.; Lacombe, F.; AVES-IMCO Team

    2001-05-01

    The NAOS adaptive optics system will very soon provide diffraction-limited images on the VLT, down to the visible wavelengths (0.020 arcseconds at 0.83 micron for instance). At the moment, the only instrument dedicated to NAOS is the CONICA spectro-imager, operating in the near-infrared from 1 to 5 microns. We are now proposing to ESO, in collaboration with an Italian group, the development of a visible spectrograph/imager/coronograph, AVES-IMCO (Adaptive Optics Visual Echelle Spectrograph and IMager/COronograph). We present here the general concept of the new instrument as well as its expected performances in the different modes.

  12. In vivo fluorescent imaging of the mouse retina using adaptive optics

    PubMed Central

    Biss, David P.; Sumorok, Daniel; Burns, Stephen A.; Webb, Robert H.; Zhou, Yaopeng; Bifano, Thomas G.; Côté, Daniel; Veilleux, Israel; Zamiri, Parisa; Lin, Charles P.

    2009-01-01

    In vivo imaging of the mouse retina using visible and near infrared wavelengths does not achieve diffraction-limited resolution due to wavefront aberrations induced by the eye. Considering the pupil size and axial dimension of the eye, it is expected that unaberrated imaging of the retina would have a transverse resolution of 2 μm. Higher-order aberrations in retinal imaging of human can be compensated for by using adaptive optics. We demonstrate an adaptive optics system for in vivo imaging of fluorescent structures in the retina of a mouse, using a microelectromechanical system membrane mirror and a Shack–Hartmann wavefront sensor that detects fluorescent wavefront. PMID:17308593

  13. [Adaptive optics for ophthalmology].

    PubMed

    Saleh, M

    2016-04-01

    Adaptive optics is a technology enhancing the visual performance of an optical system by correcting its optical aberrations. Adaptive optics have already enabled several breakthroughs in the field of visual sciences, such as improvement of visual acuity in normal and diseased eyes beyond physiologic limits, and the correction of presbyopia. Adaptive optics technology also provides high-resolution, in vivo imaging of the retina that may eventually help to detect the onset of retinal conditions at an early stage and provide better assessment of treatment efficacy.

  14. High-accuracy wavefront control for retinal imaging with Adaptive-Influence-Matrix Adaptive Optics

    PubMed Central

    Zou, Weiyao; Burns, Stephen A.

    2010-01-01

    We present an iterative technique for improving adaptive optics (AO) wavefront correction for retinal imaging, called the Adaptive-Influence-Matrix (AIM) method. This method is based on the fact that the deflection-to-voltage relation of common deformable mirrors used in AO are nonlinear, and the fact that in general the wavefront errors of the eye can be considered to be composed of a static, non-zero wavefront error (such as the defocus and astigmatism), and a time-varying wavefront error. The aberrated wavefront is first corrected with a generic influence matrix, providing a mirror compensation figure for the static wavefront error. Then a new influence matrix that is more accurate for the specific static wavefront error is calibrated based on the mirror compensation figure. Experimental results show that with the AIM method the AO wavefront correction accuracy can be improved significantly in comparison to the generic AO correction. The AIM method is most useful in AO modalities where there are large static contributions to the wavefront aberrations. PMID:19997241

  15. Adaptive optics-assisted optical coherence tomography for imaging of patients with age related macular degeneration

    NASA Astrophysics Data System (ADS)

    Sudo, Kenta; Cense, Barry

    2013-03-01

    We developed an optical coherence tomography (OCT) prototype with a sample arm that uses a 3.4 mm beam, which is considerably larger than the 1.2 to 1.5 mm beam that is used in commercialized OCT systems. The system is equipped with adaptive optics (AO), and to distinguish it from traditional AO-OCT systems with a larger 6 mm beam we have coined this concept AO-assisted OCT. Compared to commercialized OCT systems, the 3.4 mm aperture combined with AO improves light collection efficiency and imaging lateral resolution. In this paper, the performance of the AOa-OCT system was compared to a standard OCT system and demonstrated for imaging of age-related macular degeneration (AMD). Measurements were performed on the retinas of three human volunteers with healthy eyes and on one eye of a patient diagnosed with AMD. The AO-assisted OCT system imaged retinal structures of healthy human eyes and a patient eye affected by AMD with higher lateral resolution and a 9° by 9° field of view. This combination of a large isoplanatic patch and high lateral resolution can be expected to fill a gap between standard OCT with a 1.2 mm beam and conventional AO-OCT with a 6 mm beam and a 1.5° by 1.5° isoplanatic patch.

  16. Integrated adaptive optics optical coherence tomography and adaptive optics scanning laser ophthalmoscope system for simultaneous cellular resolution in vivo retinal imaging.

    PubMed

    Zawadzki, Robert J; Jones, Steven M; Pilli, Suman; Balderas-Mata, Sandra; Kim, Dae Yu; Olivier, Scot S; Werner, John S

    2011-06-01

    We describe an ultrahigh-resolution (UHR) retinal imaging system that combines adaptive optics Fourier-domain optical coherence tomography (AO-OCT) with an adaptive optics scanning laser ophthalmoscope (AO-SLO) to allow simultaneous data acquisition by the two modalities. The AO-SLO subsystem was integrated into the previously described AO-UHR OCT instrument with minimal changes to the latter. This was done in order to ensure optimal performance and image quality of the AO- UHR OCT. In this design both imaging modalities share most of the optical components including a common AO-subsystem and vertical scanner. One of the benefits of combining Fd-OCT with SLO includes automatic co-registration between two acquisition channels for direct comparison between retinal structures imaged by both modalities (e.g., photoreceptor mosaics or microvasculature maps). Because of differences in the detection scheme of the two systems, this dual imaging modality instrument can provide insight into retinal morphology and potentially function, that could not be accessed easily by a single system. In this paper we describe details of the components and parameters of the combined instrument, including incorporation of a novel membrane magnetic deformable mirror with increased stroke and actuator count used as a single wavefront corrector. We also discuss laser safety calculations for this multimodal system. Finally, retinal images acquired in vivo with this system are presented.

  17. Live imaging using adaptive optics with fluorescent protein guide-stars

    PubMed Central

    Tao, Xiaodong; Crest, Justin; Kotadia, Shaila; Azucena, Oscar; Chen, Diana C.; Sullivan, William; Kubby, Joel

    2012-01-01

    Spatially and temporally dependent optical aberrations induced by the inhomogeneous refractive index of live samples limit the resolution of live dynamic imaging. We introduce an adaptive optical microscope with a direct wavefront sensing method using a Shack-Hartmann wavefront sensor and fluorescent protein guide-stars for live imaging. The results of imaging Drosophila embryos demonstrate its ability to correct aberrations and achieve near diffraction limited images of medial sections of large Drosophila embryos. GFP-polo labeled centrosomes can be observed clearly after correction but cannot be observed before correction. Four dimensional time lapse images are achieved with the correction of dynamic aberrations. These studies also demonstrate that the GFP-tagged centrosome proteins, Polo and Cnn, serve as excellent biological guide-stars for adaptive optics based microscopy. PMID:22772285

  18. High Resolution Near-Infrared Imaging with Tip - Adaptive Optics.

    NASA Astrophysics Data System (ADS)

    Close, Laird Miller

    1995-01-01

    The development and design of the first operational tip-tilt Cassegrain secondary mirror are presented. This system, FASTTRAC, samples image motion at up to 50 Hz by tracking either infrared (m_{k } <=q 11) or visible (mR <=q 16) guide stars up to 30" and 90" away from the science target respectively. The Steward Observatory 2.3m or 1.5m telescope secondaries act as rapid tip-tilt mirrors to stabilize image motion (<=q0.1" rms;~5 Hz -3 dB frequency) based on the motion of the guide star. FASTTRAC obtains nearly diffraction-limited resolutions in seeing conditions where D/r_circ < 4 in agreement with theoretical expectations. FASTTRAC's unique ability to guide on infrared stars has allowed the first adaptively corrected images of the heavily extincted Galactic Center to be obtained. Over a hundred excellent (0.28" < FWHM < 0.6") images have been obtained of this region. These images do not detect any long term variations in the massive black hole candidate Sgr A*'s luminosity from June 1993 to September 1995. The average infrared magnitudes observed are K = 12.1 +/- 0.3, H = 13.7 +/- 0.3 and J = 16.6 +/- 0.4 integrated over 0.5" at the position of Sgr A*. No significant rapid periodicities were observed from Sgr A* for amplitudes >=q50% of the mean flux in the period range of 3-30 minutes. It is confirmed in the latest 0.28" FWHM image that there is 0.5" "bar" of emission running East-West at the position of Sgr A* as was earlier seen by Eckart et al. 1993. The observed fluxes are consistent with an inclined accretion disk around a ~1 times 10^6 M _odot black hole. However, they are also explained by a line of hot luminous (integrated luminosity of ~10^{3.5 -4.6}L_odot) central cluster stars positionally coincident with Sgr A* naturally explaining the observed 0.5" "bar". High-resolution images with FASTTRAC guiding on a faint (R = 16) visible guide star, combined with spectra from the MMT, have shown that IRAS FSC 10214 + 4724 (z = 2.28) gains its uniquely large

  19. Progress on developing wavefront sensorless adaptive optics optical coherence tomography for in vivo retinal imaging in mice

    NASA Astrophysics Data System (ADS)

    Zam, Azhar; Zhang, Pengfei; Jian, Yifan; Sarunic, Marinko V.; Bonora, Stefano; Pugh, Edward N.; Zawadzki, Robert J.

    2015-03-01

    We present a new design for a wavefront sensorless adaptive optics (WS-AO) Fourier domain optical coherence tomography (FD-OCT) system for small animal retinal imaging in vivo. Without the optical complications necessary for inclusion of a wavefront sensor in the optical system, this version of WS-AO FD-OCT system has a simplified optical design, including elimination of long focal length scanning optics and optical conjugation of vertical and horizontal scanners. This modification provides a modular large Field of View for retinal screening (25 degree visual angle), while also allowing a "zoom" capability for allocating all the scanning resources to a smaller region of interest, allowing high resolution aberration-corrected imaging. In the present system we used a 0 Dpt contact lens to stabilize the mouse eye position and to allow long duration imaging. Defocus (axial focus position) in our system is controlled by the collimation of the OCT sample arm entrance beam.

  20. Final Report: Deconvolution of Adaptive Optics Images of Titan, Neptune, and Uranus

    SciTech Connect

    Gibbard, S; Marchis, F

    2002-12-20

    This project involved images of Titan, Neptune, and Uranus obtained using the 10-meter W.M. Keck II Telescope and its adaptive optics system. An adaptive optics system corrects for turbulence in the Earth's atmosphere by sampling the wavefront and applying a correction based on the distortion measured for a known source within the same isoplanatic patch as the science target (for example, a point source such as a star). Adaptive optics can achieve a 10-fold increase in resolution over that obtained by images without adaptive optics (for example, Saturn's largest moon Titan is unresolved without adaptive optics but at least 10 resolution elements can be obtained across the disk in Keck adaptive optics images). The adaptive optics correction for atmospheric turbulence is not perfect; a point source is converted to a diffraction-limited core surrounded by a ''halo''. This halo is roughly the size and shape of the uncorrected point spread function one would observe without adaptive optics. In order to enhance the sharpness of the Keck images it is necessary to apply a deconvolution algorithm to the data. Many such deconvolution algorithms exist such as maximum likelihood and maximum entropy. These algorithms suffer to various degrees from noise amplification and creation of artifacts near sharp edges (''ringing''). In order to deconvolve the Keck images I have applied an algorithm specifically developed for observations of planetary bodies, the myopic deconvolution algorithm MISTRAL (''Myopic Iterative STep-preserving Restoration ALgorithm'') (Conan et al. 1998, 2000). MISTRAL was developed by ONERA (Office National d'Etudes et de Recherches Aerospatiales) and has been extensively tested on simulated and real AO observations, including observations of Titan (Coustenis et al.2001), Io (Marchis et al.2002, 2001), and asteroids (Hestroffer et al.2001, Rosenberg et al.2001, Makhoul et al.2001). Compared to more classical methods, MISTRAL avoids noise amplification and

  1. LGSD/NGSD: high speed optical CMOS imagers for E-ELT adaptive optics

    NASA Astrophysics Data System (ADS)

    Downing, Mark; Kolb, Johann; Balard, Philippe; Dierickx, Bart; Defernez, Arnaud; Feautrier, Philippe; Finger, Gert; Fryer, Martin; Gach, Jean-Luc; Guillaume, Christian; Hubin, Norbert; Jerram, Paul; Jorden, Paul; Meyer, Manfred; Payne, Andrew; Pike, Andrew; Reyes, Javier; Simpson, Robert; Stadler, Eric; Stent, Jeremy; Swift, Nick

    2014-07-01

    The success of the next generation of instruments for ELT class telescopes will depend upon improving the image quality by exploiting sophisticated Adaptive Optics (AO) systems. One of the critical components of the AO systems for the E-ELT has been identified as the optical Laser/Natural Guide Star WFS detector. The combination of large format, 1760×1680 pixels to finely sample the wavefront and the spot elongation of laser guide stars, fast frame rate of 700 frames per second (fps), low read noise (< 3e-), and high QE (> 90%) makes the development of this device extremely challenging. Design studies concluded that a highly integrated Backside Illuminated CMOS Imager built on High Resistivity silicon as the most likely technology to succeed. Two generations of the CMOS Imager are being developed: a) the already designed and manufactured NGSD (Natural Guide Star Detector), a quarter-sized pioneering device of 880×840 pixels capable of meeting first light needs of the E-ELT; b) the LGSD (Laser Guide Star Detector), the larger full size device. The detailed design is presented including the approach of using massive parallelism (70,400 ADCs) to achieve the low read noise at high pixel rates of ~3 Gpixel/s and the 88 channel LVDS 220Mbps serial interface to get the data off-chip. To enable read noise closer to the goal of 1e- to be achieved, a split wafer run has allowed the NGSD to be manufactured in the more speculative, but much lower read noise, Ultra Low Threshold Transistors in the unit cell. The NGSD has come out of production, it has been thinned to 12μm, backside processed and packaged in a custom 370pin Ceramic PGA (Pin Grid Array). First results of tests performed both at e2v and ESO are presented.

  2. Imaging human retinal pigment epithelium cells using adaptive optics optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Liu, Zhuolin; Kocaoglu, Omer P.; Turner, Timothy L.; Miller, Donald T.

    2016-03-01

    Retinal pigment epithelium (RPE) cells are vital to health of the outer retina, but are often compromised in ageing and major ocular diseases that lead to blindness. Early manifestation of RPE disruption occurs at the cellular level, and while biomarkers at this scale hold considerable promise, RPE cells have proven extremely challenging to image in the living human eye. We present a novel method based on optical coherence tomography (OCT) equipped with adaptive optics (AO) that overcomes the associated technical obstacles. The method takes advantage of the 3D resolution of AO-OCT, but more critically sub-cellular segmentation and registration that permit organelle motility to be used as a novel contrast mechanism. With this method, we successfully visualized RPE cells and characterized their 3D reflectance profile in every subject and retinal location (3° and 7° temporal to the fovea) imaged to date. We have quantified RPE packing geometry in terms of cell density, cone-to-RPE ratio, and number of nearest neighbors using Voronoi and power spectra analyses. RPE cell density (cells/mm2) showed no significant difference between 3° (4,892+/-691) and 7° (4,780+/-354). In contrast, cone-to- RPE ratio was significantly higher at 3° (3.88+/-0.52:1) than 7° (2.31+/- 0.23:1). Voronoi analysis also showed most RPE cells have six nearest neighbors, which was significantly larger than the next two most prevalent associations: five and seven. Averaged across the five subjects, prevalence of cells with six neighbors was 51.4+/-3.58% at 3°, and 54.58+/-3.01% at 7°. These results are consistent with histology and in vivo studies using other imaging modalities.

  3. Accuracy requirements of optical linear algebra processors in adaptive optics imaging systems

    NASA Technical Reports Server (NTRS)

    Downie, John D.; Goodman, Joseph W.

    1989-01-01

    The accuracy requirements of optical processors in adaptive optics systems are determined by estimating the required accuracy in a general optical linear algebra processor (OLAP) that results in a smaller average residual aberration than that achieved with a conventional electronic digital processor with some specific computation speed. Special attention is given to an error analysis of a general OLAP with regard to the residual aberration that is created in an adaptive mirror system by the inaccuracies of the processor, and to the effect of computational speed of an electronic processor on the correction. Results are presented on the ability of an OLAP to compete with a digital processor in various situations.

  4. IMAGING WITH MULTIMODAL ADAPTIVE-OPTICS OPTICAL COHERENCE TOMOGRAPHY IN MULTIPLE EVANESCENT WHITE DOT SYNDROME: THE STRUCTURE AND FUNCTIONAL RELATIONSHIP

    PubMed Central

    Legarreta, Andrew D.; Legarreta, John E.; Nadler, Zach; Gallagher, Denise; Hammer, Daniel X.; Ferguson, R. Daniel; Iftimia, Nicusor; Wollstein, Gadi; Schuman, Joel S.

    2016-01-01

    Purpose: To elucidate the location of pathological changes in multiple evanescent white dot syndrome (MEWDS) with the use of multimodal adaptive optics (AO) imaging. Methods: A 5-year observational case study of a 24-year-old female with recurrent MEWDS. Full examination included history, Snellen chart visual acuity, pupil assessment, intraocular pressures, slit lamp evaluation, dilated fundoscopic exam, imaging with Fourier-domain optical coherence tomography (FD-OCT), blue-light fundus autofluorescence (FAF), fundus photography, fluorescein angiography, and adaptive-optics optical coherence tomography. Results: Three distinct acute episodes of MEWDS occurred during the period of follow-up. Fourier-domain optical coherence tomography and adaptive-optics imaging showed disturbance in the photoreceptor outer segments (PR OS) in the posterior pole with each flare. The degree of disturbance at the photoreceptor level corresponded to size and extent of the visual field changes. All findings were transient with delineation of the photoreceptor recovery from the outer edges of the lesion inward. Hyperautofluorescence was seen during acute flares. Increase in choroidal thickness did occur with each active flare but resolved. Conclusion: Although changes in the choroid and RPE can be observed in MEWDS, Fourier-domain optical coherence tomography, and multimodal adaptive optics imaging localized the visually significant changes seen in this disease at the level of the photoreceptors. These transient retinal changes specifically occur at the level of the inner segment ellipsoid and OS/RPE line. En face optical coherence tomography imaging provides a detailed, yet noninvasive method for following the convalescence of MEWDS and provides insight into the structural and functional relationship of this transient inflammatory retinal disease. PMID:26735319

  5. Stokes vector analysis of adaptive optics images of the retina.

    PubMed

    Song, Hongxin; Zhao, Yanming; Qi, Xiaofeng; Chui, Yuenping Toco; Burns, Stephen A

    2008-01-15

    A high-resolution Stokes vector imaging polarimeter was developed to measure the polarization properties at the cellular level in living human eyes. The application of this cellular level polarimetric technique to in vivo retinal imaging has allowed us to measure depolarization in the retina and to improve the retinal image contrast of retinal structures based on their polarization properties. PMID:18197217

  6. Volumetric imaging of inner retina with adaptive optics spectral-domain optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Zhang, Yan, II; Cense, Barry; Jonnal, Ravi S.; Gao, Weihua; Jones, Steve; Olivier, Scot; Miller, Donald T.

    2007-02-01

    Adaptive optics (AO) coupled with ultra-fast spectral-domain optical coherence tomography (SD-OCT) has achieved the necessary 3D resolution, sensitivity, and speed for imaging the microscopic retina at the cellular level. While this technology has been rigorously applied to evaluating the 3D morphology of cone photoreceptors, similar detailed studies of cell-sized structures in the inner retina have yet to be undertaken. In this paper, we improve the technical performance of our AO ultrafast SD-OCT and investigate its use for imaging the microscopic inner retina, in particular the nerve fiber layer (NFL) and retinal capillary network. To maximize lateral resolution within the inner retina, focus was controlled with a high stroke, 37-actuator bimorph mirror (AOptix) that also served as the wavefront corrector of the AO. The AO system operated at a closed-loop rate of 25 Hz. The SD-OCT sub-system consisted of a superluminescent diode (λ= 842 nm, Δλ= 50 nm) and a 512 pixel line scan charge-coupled device (CCD) that acquired 72,000 A-scans/sec. Three different B-scan lengths (36, 60, and 120 A-scans/B-scan), which correspond to B-scan exposure durations of 0.5, 0.83, and 1.67 ms, were evaluated to determine the maximum B-scan length that could be tolerated without noticeable loss in image quality due to eye motion in the well fixated eye. Additional technical improvements included sub-pixel registration to remove instrument error and axial registration of the volume images. Small volume images were acquired at 2 and 7 degrees retinal eccentricity with focus systematically shifted through the retina. Small capillaries, some approaching the smallest in the human eye, were readily detected with AO SD-OCT. Appearance of the nerve fiber layer varied noticeably with depth. The most inner portion (presumably the inner limiting membrane) appeared as a thin irregular surface with little characteristic speckle noise. Within the NFL, complex striation patterns (presumably NFL

  7. Retinal axial focusing and multi-layer imaging with a liquid crystal adaptive optics camera

    NASA Astrophysics Data System (ADS)

    Liu, Rui-Xue; Zheng, Xian-Liang; Li, Da-Yu; Xia, Ming-Liang; Hu, Li-Fa; Cao, Zhao-Liang; Mu, Quan-Quan; Xuan, Li

    2014-09-01

    With the help of adaptive optics (AO) technology, cellular level imaging of living human retina can be achieved. Aiming to reduce distressing feelings and to avoid potential drug induced diseases, we attempted to image retina with dilated pupil and froze accommodation without drugs. An optimized liquid crystal adaptive optics camera was adopted for retinal imaging. A novel eye stared system was used for stimulating accommodation and fixating imaging area. Illumination sources and imaging camera kept linkage for focusing and imaging different layers. Four subjects with diverse degree of myopia were imaged. Based on the optical properties of the human eye, the eye stared system reduced the defocus to less than the typical ocular depth of focus. In this way, the illumination light can be projected on certain retina layer precisely. Since that the defocus had been compensated by the eye stared system, the adopted 512 × 512 liquid crystal spatial light modulator (LC-SLM) corrector provided the crucial spatial fidelity to fully compensate high-order aberrations. The Strehl ratio of a subject with -8 diopter myopia was improved to 0.78, which was nearly close to diffraction-limited imaging. By finely adjusting the axial displacement of illumination sources and imaging camera, cone photoreceptors, blood vessels and nerve fiber layer were clearly imaged successfully.

  8. Adaptive optics instrument for long-range imaging. Final report

    SciTech Connect

    Crawford, T.M.

    1998-06-01

    The science and history of imaging through a turbulent atmosphere is reviewed in detail. Traditional methods for reducing the effects of turbulence are presented. A simplified method for turbulence reduction called the Sheared Coherent Interferometric Photography (SCIP) method is presented. Implementation of SCIP is discussed along with experimental results. Limitations in the use of this method are discussed along with recommendations for future improvements.

  9. Adaptive optics imaging of the outer retinal tubules in Bietti's crystalline dystrophy.

    PubMed

    Battu, R; Akkali, M C; Bhanushali, D; Srinivasan, P; Shetty, R; Berendschot, T T J M; Schouten, J S A G; Webers, C A

    2016-05-01

    PurposeTo study the outer retinal tubules using spectral domain optical coherence tomography and adaptive optics and in patients with Bietti's crystalline dystrophy.MethodsTen eyes of five subjects from five independent families with Bietti's crystalline Dystrophy (BCD) were characterized with best-corrected visual acuity (BCVA), full-field electroretinography, and fundus autofluorescence (FAF). High-resolution images were obtained with the spectral domain optical coherence tomography (SD-OCT) and adaptive optics (AO).ResultsSD-OCT showed prominent outer retinal layer loss and outer retinal tubulations at the margin of outer retinal loss. AO images displayed prominent macrotubules and microtubules with characteristic features in eight out of the 10 eyes. Crystals were present in all ten eyes. There was a reduction in the cone count in all eyes in the area outside the outer retinal tubules (ORT).ConclusionsThis study describes the morphology of the outer retinal tubules when imaged enface on the adaptive optics in patients with BCD. These findings provide insight into the macular structure of these patients. This may have prognostic implications and refine the study on the pathogenesis of BCD.

  10. Wavefront sensorless approaches to adaptive optics for in vivo fluorescence imaging of mouse retina

    NASA Astrophysics Data System (ADS)

    Wahl, Daniel J.; Bonora, Stefano; Mata, Oscar S.; Haunerland, Bengt K.; Zawadzki, Robert J.; Sarunic, Marinko V.; Jian, Yifan

    2016-03-01

    Adaptive optics (AO) is necessary to correct aberrations when imaging the mouse eye with high numerical aperture. In order to obtain cellular resolution, we have implemented wavefront sensorless adaptive optics for in vivo fluorescence imaging of mouse retina. Our approach includes a lens-based system and MEMS deformable mirror for aberration correction. The AO system was constructed with a reflectance channel for structural images and fluorescence channel for functional images. The structural imaging was used in real-time for navigation on the retina using landmarks such as blood vessels. We have also implemented a tunable liquid lens to select the retinal layer of interest at which to perform the optimization. At the desired location on the mouse retina, the optimization algorithm used the fluorescence image data to drive a modal hill-climbing algorithm using an intensity or sharpness image quality metric. The optimization requires ~30 seconds to complete a search up to the 20th Zernike mode. In this report, we have demonstrated the AO performance for high-resolution images of the capillaries in a fluorescence angiography. We have also made progress on an approach to AO with pupil segmentation as a possible sensorless technique suitable for small animal retinal imaging. Pupil segmentation AO was implemented on the same ophthalmic system and imaging performance was demonstrated on fluorescent beads with induced aberrations.

  11. MEMS Deformable Mirrors for Adaptive Optics in Astronomical Imaging

    NASA Astrophysics Data System (ADS)

    Cornelissen, S.; Bierden, P. A.; Bifano, T.

    We report on the development of micro-electromechanical (MEMS) deformable mirrors designed for ground and space-based astronomical instruments intended for imaging extra-solar planets. Three different deformable mirror designs, a 1024 element continuous membrane (32x32), a 4096 element continuous membrane (64x64), and a 331 hexagonal segmented tip-tilt-piston are being produced for the Planet Imaging Concept Testbed Using a Rocket Experiment (PICTURE) program, the Gemini Planet Imaging Instrument, and the visible nulling coronograph developed at JPL for NASA's TPF mission, respectively. The design of these polysilicon, surface-micromachined MEMS deformable mirrors builds on technology that was pioneered at Boston University and has been used extensively to correct for ocular aberrations in retinal imaging systems and for compensation of atmospheric turbulence in free-space laser communication. These light-weight, low power deformable mirrors will have an active aperture of up to 25.2mm consisting of thin silicon membrane mirror supported by an array of 1024 to 4096 electrostatic actuators exhibiting no hysteresis and sub-nanometer repeatability. The continuous membrane deformable mirrors, coated with a highly reflective metal film, will be capable of up to 4μm of stroke, have a surface finish of <10nm RMS with a fill factor of 99.8%. The segmented device will have a range of motion of 1um of piston and a 600 arc-seconds of tip/tilt simultaneously and a surface finish of 1nm RMS. The individual mirror elements in this unique device, are designed such that they will maintain their flatness throughout the range of travel. New design features and fabrication processes are combined with a proven device architecture to achieve the desired performance and high reliability. Presented in this paper are device characteristic and performance results of these devices.

  12. Adaptive optics scanning laser ophthalmoscope with integrated wide-field retinal imaging and tracking

    PubMed Central

    Ferguson, R. Daniel; Zhong, Zhangyi; Hammer, Daniel X.; Mujat, Mircea; Patel, Ankit H.; Deng, Cong; Zou, Weiyao; Burns, Stephen A.

    2010-01-01

    We have developed a new, unified implementation of the adaptive optics scanning laser ophthalmoscope (AOSLO) incorporating a wide-field line-scanning ophthalmoscope (LSO) and a closed-loop optical retinal tracker. AOSLO raster scans are deflected by the integrated tracking mirrors so that direct AOSLO stabilization is automatic during tracking. The wide-field imager and large-spherical-mirror optical interface design, as well as a large-stroke deformable mirror (DM), enable the AOSLO image field to be corrected at any retinal coordinates of interest in a field of >25 deg. AO performance was assessed by imaging individuals with a range of refractive errors. In most subjects, image contrast was measurable at spatial frequencies close to the diffraction limit. Closed-loop optical (hardware) tracking performance was assessed by comparing sequential image series with and without stabilization. Though usually better than 10 μm rms, or 0.03 deg, tracking does not yet stabilize to single cone precision but significantly improves average image quality and increases the number of frames that can be successfully aligned by software-based post-processing methods. The new optical interface allows the high-resolution imaging field to be placed anywhere within the wide field without requiring the subject to re-fixate, enabling easier retinal navigation and faster, more efficient AOSLO montage capture and stitching. PMID:21045887

  13. Images of photoreceptors in living primate eyes using adaptive optics two-photon ophthalmoscopy

    PubMed Central

    Hunter, Jennifer J.; Masella, Benjamin; Dubra, Alfredo; Sharma, Robin; Yin, Lu; Merigan, William H.; Palczewska, Grazyna; Palczewski, Krzysztof; Williams, David R.

    2011-01-01

    In vivo two-photon imaging through the pupil of the primate eye has the potential to become a useful tool for functional imaging of the retina. Two-photon excited fluorescence images of the macaque cone mosaic were obtained using a fluorescence adaptive optics scanning laser ophthalmoscope, overcoming the challenges of a low numerical aperture, imperfect optics of the eye, high required light levels, and eye motion. Although the specific fluorophores are as yet unknown, strong in vivo intrinsic fluorescence allowed images of the cone mosaic. Imaging intact ex vivo retina revealed that the strongest two-photon excited fluorescence signal comes from the cone inner segments. The fluorescence response increased following light stimulation, which could provide a functional measure of the effects of light on photoreceptors. PMID:21326644

  14. Determining the imaging plane of a retinal capillary layer in adaptive optical imaging

    NASA Astrophysics Data System (ADS)

    Yang, Le-Bao; Hu, Li-Fa; Li, Da-Yu; Cao, Zhao-Liang; Mu, Quan-Quan; Ma, Ji; Xuan, Li

    2016-09-01

    Even in the early stage, endocrine metabolism disease may lead to micro aneurysms in retinal capillaries whose diameters are less than 10 μm. However, the fundus cameras used in clinic diagnosis can only obtain images of vessels larger than 20 μm in diameter. The human retina is a thin and multiple layer tissue, and the layer of capillaries less than 10 μm in diameter only exists in the inner nuclear layer. The layer thickness of capillaries less than 10 μm in diameter is about 40 μm and the distance range to rod&cone cell surface is tens of micrometers, which varies from person to person. Therefore, determining reasonable capillary layer (CL) position in different human eyes is very difficult. In this paper, we propose a method to determine the position of retinal CL based on the rod&cone cell layer. The public positions of CL are recognized with 15 subjects from 40 to 59 years old, and the imaging planes of CL are calculated by the effective focal length of the human eye. High resolution retinal capillary imaging results obtained from 17 subjects with a liquid crystal adaptive optics system (LCAOS) validate our method. All of the subjects’ CLs have public positions from 127 μm to 147 μm from the rod&cone cell layer, which is influenced by the depth of focus. Project supported by the National Natural Science Foundation of China (Grant Nos. 11174274, 11174279, 61205021, 11204299, 61475152, and 61405194).

  15. Determining the imaging plane of a retinal capillary layer in adaptive optical imaging

    NASA Astrophysics Data System (ADS)

    Yang, Le-Bao; Hu, Li-Fa; Li, Da-Yu; Cao, Zhao-Liang; Mu, Quan-Quan; Ma, Ji; Xuan, Li

    2016-09-01

    Even in the early stage, endocrine metabolism disease may lead to micro aneurysms in retinal capillaries whose diameters are less than 10 μm. However, the fundus cameras used in clinic diagnosis can only obtain images of vessels larger than 20 μm in diameter. The human retina is a thin and multiple layer tissue, and the layer of capillaries less than 10 μm in diameter only exists in the inner nuclear layer. The layer thickness of capillaries less than 10 μm in diameter is about 40 μm and the distance range to rod&cone cell surface is tens of micrometers, which varies from person to person. Therefore, determining reasonable capillary layer (CL) position in different human eyes is very difficult. In this paper, we propose a method to determine the position of retinal CL based on the rod&cone cell layer. The public positions of CL are recognized with 15 subjects from 40 to 59 years old, and the imaging planes of CL are calculated by the effective focal length of the human eye. High resolution retinal capillary imaging results obtained from 17 subjects with a liquid crystal adaptive optics system (LCAOS) validate our method. All of the subjects’ CLs have public positions from 127 μm to 147 μm from the rod&cone cell layer, which is influenced by the depth of focus. Project supported by the National Natural Science Foundation of China (Grant Nos. 11174274, 11174279, 61205021, 11204299, 61475152, and 61405194).

  16. Photoreceptor counting and montaging of en-face retinal images from an adaptive optics fundus camera.

    PubMed

    Xue, Bai; Choi, Stacey S; Doble, Nathan; Werner, John S

    2007-05-01

    A fast and efficient method for quantifying photoreceptor density in images obtained with an en-face flood-illuminated adaptive optics (AO) imaging system is described. To improve accuracy of cone counting, en-face images are analyzed over extended areas. This is achieved with two separate semiautomated algorithms: (1) a montaging algorithm that joins retinal images with overlapping common features without edge effects and (2) a cone density measurement algorithm that counts the individual cones in the montaged image. The accuracy of the cone density measurement algorithm is high, with >97% agreement for a simulated retinal image (of known density, with low contrast) and for AO images from normal eyes when compared with previously reported histological data. Our algorithms do not require spatial regularity in cone packing and are, therefore, useful for counting cones in diseased retinas, as demonstrated for eyes with Stargardt's macular dystrophy and retinitis pigmentosa. PMID:17429482

  17. Registration of adaptive optics corrected retinal nerve fiber layer (RNFL) images.

    PubMed

    Ramaswamy, Gomathy; Lombardo, Marco; Devaney, Nicholas

    2014-06-01

    Glaucoma is the leading cause of preventable blindness in the western world. Investigation of high-resolution retinal nerve fiber layer (RNFL) images in patients may lead to new indicators of its onset. Adaptive optics (AO) can provide diffraction-limited images of the retina, providing new opportunities for earlier detection of neuroretinal pathologies. However, precise processing is required to correct for three effects in sequences of AO-assisted, flood-illumination images: uneven illumination, residual image motion and image rotation. This processing can be challenging for images of the RNFL due to their low contrast and lack of clearly noticeable features. Here we develop specific processing techniques and show that their application leads to improved image quality on the nerve fiber bundles. This in turn improves the reliability of measures of fiber texture such as the correlation of Gray-Level Co-occurrence Matrix (GLCM).

  18. Registration of adaptive optics corrected retinal nerve fiber layer (RNFL) images

    PubMed Central

    Ramaswamy, Gomathy; Lombardo, Marco; Devaney, Nicholas

    2014-01-01

    Glaucoma is the leading cause of preventable blindness in the western world. Investigation of high-resolution retinal nerve fiber layer (RNFL) images in patients may lead to new indicators of its onset. Adaptive optics (AO) can provide diffraction-limited images of the retina, providing new opportunities for earlier detection of neuroretinal pathologies. However, precise processing is required to correct for three effects in sequences of AO-assisted, flood-illumination images: uneven illumination, residual image motion and image rotation. This processing can be challenging for images of the RNFL due to their low contrast and lack of clearly noticeable features. Here we develop specific processing techniques and show that their application leads to improved image quality on the nerve fiber bundles. This in turn improves the reliability of measures of fiber texture such as the correlation of Gray-Level Co-occurrence Matrix (GLCM). PMID:24940551

  19. Enhancement and bias removal of optical coherence tomography images: An iterative approach with adaptive bilateral filtering.

    PubMed

    Sudeep, P V; Issac Niwas, S; Palanisamy, P; Rajan, Jeny; Xiaojun, Yu; Wang, Xianghong; Luo, Yuemei; Liu, Linbo

    2016-04-01

    Optical coherence tomography (OCT) has continually evolved and expanded as one of the most valuable routine tests in ophthalmology. However, noise (speckle) in the acquired images causes quality degradation of OCT images and makes it difficult to analyze the acquired images. In this paper, an iterative approach based on bilateral filtering is proposed for speckle reduction in multiframe OCT data. Gamma noise model is assumed for the observed OCT image. First, the adaptive version of the conventional bilateral filter is applied to enhance the multiframe OCT data and then the bias due to noise is reduced from each of the filtered frames. These unbiased filtered frames are then refined using an iterative approach. Finally, these refined frames are averaged to produce the denoised OCT image. Experimental results on phantom images and real OCT retinal images demonstrate the effectiveness of the proposed filter. PMID:26907572

  20. Visible Light Adaptive Optics Imaging of the Orion 218-354 Silhouette Disk

    NASA Astrophysics Data System (ADS)

    Follette, Katherine B.; Close, Laird M.; Males, Jared R.; Kopon, Derek; Wu, Ya-Lin; Morzinski, Katie M.; Hinz, Philip; Rodigas, Timothy J.; Puglisi, Alfio; Esposito, Simone; Riccardi, Armando; Pinna, Enrico; Xompero, Marco; Briguglio, Runa

    2014-01-01

    We present the first ground-based adaptive optics images of a silhouette disk. This disk, Orion 218-354, is seen in silhouette against the bright nebular background of Orion, and was resolved using the new Magellan Adaptive Secondary AO system and its VisAO camera in Simultaneous Differential Imaging (SDI) mode. PSF subtraction of Orion 218-354 reveals a disk ~1'' (400 AU) in radius, with the degree of absorption increasing steadily towards the center of the disk. By virtue of the central star being unsaturated, these data probe inward to a much smaller radius than previous HST images. Our data present a different picture than previous observers had hypothesized, namely that the disk is likely optically thin at Hα at least as far inward as ~20AU. In addition to being among the first high-resolution AO images taken in the optical on a large telescope, these data reveal the power of SDI imaging to illuminate disk structure, and speak to a bright future for visible AO imaging. Analysis of the results described briefly here can be found in full detail in Follette et al. (2013).

  1. An adaptive-optics scanning laser ophthalmoscope for imaging murine retinal microstructure

    NASA Astrophysics Data System (ADS)

    Alt, Clemens; Biss, David P.; Tajouri, Nadja; Jakobs, Tatjana C.; Lin, Charles P.

    2010-02-01

    In vivo retinal imaging is an outstanding tool to observe biological processes unfold in real-time. The ability to image microstructure in vivo can greatly enhance our understanding of function in retinal microanatomy under normal conditions and in disease. Transgenic mice are frequently used for mouse models of retinal diseases. However, commercially available retinal imaging instruments lack the optical resolution and spectral flexibility necessary to visualize detail comprehensively. We developed an adaptive optics scanning laser ophthalmoscope (AO-SLO) specifically for mouse eyes. Our SLO is a sensor-less adaptive optics system (no Shack Hartmann sensor) that employs a stochastic parallel gradient descent algorithm to modulate a deformable mirror, ultimately aiming to correct wavefront aberrations by optimizing confocal image sharpness. The resulting resolution allows detailed observation of retinal microstructure. The AO-SLO can resolve retinal microglia and their moving processes, demonstrating that microglia processes are highly motile, constantly probing their immediate environment. Similarly, retinal ganglion cells are imaged along with their axons and sprouting dendrites. Retinal blood vessels are imaged both using evans blue fluorescence and backscattering contrast.

  2. Wavefront sensorless adaptive optics fluorescence biomicroscope for in vivo retinal imaging in mice.

    PubMed

    Wahl, Daniel J; Jian, Yifan; Bonora, Stefano; Zawadzki, Robert J; Sarunic, Marinko V

    2016-01-01

    Cellular-resolution in vivo fluorescence imaging is a valuable tool for longitudinal studies of retinal function in vision research. Wavefront sensorless adaptive optics (WSAO) is a developing technology that enables high-resolution imaging of the mouse retina. In place of the conventional method of using a Shack-Hartmann wavefront sensor to measure the aberrations directly, WSAO uses an image quality metric and a search algorithm to drive the shape of the adaptive element (i.e. deformable mirror). WSAO is a robust approach to AO and it is compatible with a compact, low-cost lens-based system. In this report, we demonstrated a hill-climbing algorithm for WSAO with a variable focus lens and deformable mirror for non-invasive in vivo imaging of EGFP (enhanced green fluorescent protein) labelled ganglion cells and microglia cells in the mouse retina.

  3. Wavefront sensorless adaptive optics fluorescence biomicroscope for in vivo retinal imaging in mice

    PubMed Central

    Wahl, Daniel J.; Jian, Yifan; Bonora, Stefano; Zawadzki, Robert J.; Sarunic, Marinko V.

    2015-01-01

    Cellular-resolution in vivo fluorescence imaging is a valuable tool for longitudinal studies of retinal function in vision research. Wavefront sensorless adaptive optics (WSAO) is a developing technology that enables high-resolution imaging of the mouse retina. In place of the conventional method of using a Shack-Hartmann wavefront sensor to measure the aberrations directly, WSAO uses an image quality metric and a search algorithm to drive the shape of the adaptive element (i.e. deformable mirror). WSAO is a robust approach to AO and it is compatible with a compact, low-cost lens-based system. In this report, we demonstrated a hill-climbing algorithm for WSAO with a variable focus lens and deformable mirror for non-invasive in vivo imaging of EGFP (enhanced green fluorescent protein) labelled ganglion cells and microglia cells in the mouse retina. PMID:26819812

  4. Sodium Laser Guide Star Adaptive Optics Imaging Polarimetry of Herbig Ae/Be Stars

    SciTech Connect

    Perrin, M D; Graham, J R; Lloyd, J P; Kalas, P; Gates, E L; Gavel, D T; Pennington, D M; Max, C E

    2004-01-08

    The future of high-resolution ground-based optical and infrared astronomy requires the successful implementation of laser guide star adaptive optics systems. We present the first science results from the Lick Observatory sodium beacon laser guide star system. By coupling this system to a near-infrared (J;H;Ks bands) dual-channel imaging polarimeter, we achieve very high sensitivity to light scattered in the circumstellar enviroment of Herbig Ae/Be stars on scales of 100-300 AU. Observations of LkH{alpha} 198 reveal a highly polarized, biconical nebula 10 arcseconds in diameter (6000 AU) . We also observe a polarized jet-like feature associated with the deeply embedded source LkH{alpha} 198-IR. The star LkH{alpha} 233 presents a narrow, unpolarized dark lane dividing its characteristic butterfly-shaped polarized reflection nebulosity. This linear structure is oriented perpendicular to an optical jet and bipolar cavity and is consistent with the presence of an optically thick circumstellar disk blocking our direct view of the star. These data suggest that the evolutionary picture developed for the lower-mass T Tauri stars is also relevant to the Herbig Ae/Be stars and demonstrate the ability of laser guide star adaptive optics systems to obtain scientific results competitive with natural guide star adaptive optics or space-based telescopes.

  5. Research on the liquid crystal adaptive optics system for human retinal imaging

    NASA Astrophysics Data System (ADS)

    Zhang, Lei; Tong, Shoufeng; Song, Yansong; Zhao, Xin

    2013-12-01

    The blood vessels only in Human eye retinal can be observed directly. Many diseases that are not obvious in their early symptom can be diagnosed through observing the changes of distal micro blood vessel. In order to obtain the high resolution human retinal images,an adaptive optical system for correcting the aberration of the human eye was designed by using the Shack-Hartmann wavefront sensor and the Liquid Crystal Spatial Light Modulator(LCLSM) .For a subject eye with 8m-1 (8D)myopia, the wavefront error is reduced to 0.084 λ PV and 0.12 λRMS after adaptive optics(AO) correction ,which has reached diffraction limit.The results show that the LCLSM based AO system has the ability of correcting the aberration of the human eye efficiently,and making the blurred photoreceptor cell to clearly image on a CCD camera.

  6. High-speed adaptive optics for imaging of the living human eye

    PubMed Central

    Yu, Yongxin; Zhang, Tianjiao; Meadway, Alexander; Wang, Xiaolin; Zhang, Yuhua

    2015-01-01

    The discovery of high frequency temporal fluctuation of human ocular wave aberration dictates the necessity of high speed adaptive optics (AO) correction for high resolution retinal imaging. We present a high speed AO system for an experimental adaptive optics scanning laser ophthalmoscope (AOSLO). We developed a custom high speed Shack-Hartmann wavefront sensor and maximized the wavefront detection speed based upon a trade-off among the wavefront spatial sampling density, the dynamic range, and the measurement sensitivity. We examined the temporal dynamic property of the ocular wavefront under the AOSLO imaging condition and improved the dual-thread AO control strategy. The high speed AO can be operated with a closed-loop frequency up to 110 Hz. Experiment results demonstrated that the high speed AO system can provide improved compensation for the wave aberration up to 30 Hz in the living human eye. PMID:26368408

  7. An optimized adaptive optics experimental setup for in vivo retinal imaging

    NASA Astrophysics Data System (ADS)

    Balderas-Mata, S. E.; Valdivieso González, L. G.; Ramírez Zavaleta, G.; López Olazagasti, E.; Tepichin Rodriguez, E.

    2012-10-01

    The use of Adaptive Optics (AO) in ophthalmologic instruments to image human retinas has been probed to improve the imaging lateral resolution, by correcting both static and dynamic aberrations inherent in human eyes. Typically, the configuration of the AO arm uses an infrared beam from a superluminescent diode (SLD), which is focused on the retina, acting as a point source. The back reflected light emerges through the eye optical system bringing with it the aberrations of the cornea. The aberrated wavefront is measured with a Shack - Hartmann wavefront sensor (SHWFS). However, the aberrations in the optical imaging system can reduced the performance of the wave front correction. The aim of this work is to present an optimized first stage AO experimental setup for in vivo retinal imaging. In our proposal, the imaging optical system has been designed in order to reduce spherical aberrations due to the lenses. The ANSI Standard is followed assuring the safety power levels. The performance of the system will be compared with a commercial aberrometer. This system will be used as the AO arm of a flood-illuminated fundus camera system for retinal imaging. We present preliminary experimental results showing the enhancement.

  8. 3-D Adaptive Sparsity Based Image Compression with Applications to Optical Coherence Tomography

    PubMed Central

    Fang, Leyuan; Li, Shutao; Kang, Xudong; Izatt, Joseph A.; Farsiu, Sina

    2015-01-01

    We present a novel general-purpose compression method for tomographic images, termed 3D adaptive sparse representation based compression (3D-ASRC). In this paper, we focus on applications of 3D-ASRC for the compression of ophthalmic 3D optical coherence tomography (OCT) images. The 3D-ASRC algorithm exploits correlations among adjacent OCT images to improve compression performance, yet is sensitive to preserving their differences. Due to the inherent denoising mechanism of the sparsity based 3D-ASRC, the quality of the compressed images are often better than the raw images they are based on. Experiments on clinical-grade retinal OCT images demonstrate the superiority of the proposed 3D-ASRC over other well-known compression methods. PMID:25561591

  9. Quality metric in matched Laplacian of Gaussian response domain for blind adaptive optics image deconvolution

    NASA Astrophysics Data System (ADS)

    Guo, Shiping; Zhang, Rongzhi; Yang, Yikang; Xu, Rong; Liu, Changhai; Li, Jisheng

    2016-04-01

    Adaptive optics (AO) in conjunction with subsequent postprocessing techniques have obviously improved the resolution of turbulence-degraded images in ground-based astronomical observations or artificial space objects detection and identification. However, important tasks involved in AO image postprocessing, such as frame selection, stopping iterative deconvolution, and algorithm comparison, commonly need manual intervention and cannot be performed automatically due to a lack of widely agreed on image quality metrics. In this work, based on the Laplacian of Gaussian (LoG) local contrast feature detection operator, we propose a LoG domain matching operation to perceive effective and universal image quality statistics. Further, we extract two no-reference quality assessment indices in the matched LoG domain that can be used for a variety of postprocessing tasks. Three typical space object images with distinct structural features are tested to verify the consistency of the proposed metric with perceptual image quality through subjective evaluation.

  10. Solar tomography adaptive optics.

    PubMed

    Ren, Deqing; Zhu, Yongtian; Zhang, Xi; Dou, Jiangpei; Zhao, Gang

    2014-03-10

    Conventional solar adaptive optics uses one deformable mirror (DM) and one guide star for wave-front sensing, which seriously limits high-resolution imaging over a large field of view (FOV). Recent progress toward multiconjugate adaptive optics indicates that atmosphere turbulence induced wave-front distortion at different altitudes can be reconstructed by using multiple guide stars. To maximize the performance over a large FOV, we propose a solar tomography adaptive optics (TAO) system that uses tomographic wave-front information and uses one DM. We show that by fully taking advantage of the knowledge of three-dimensional wave-front distribution, a classical solar adaptive optics with one DM can provide an extra performance gain for high-resolution imaging over a large FOV in the near infrared. The TAO will allow existing one-deformable-mirror solar adaptive optics to deliver better performance over a large FOV for high-resolution magnetic field investigation, where solar activities occur in a two-dimensional field up to 60'', and where the near infrared is superior to the visible in terms of magnetic field sensitivity.

  11. The Adaptive Optics Lucky Imager: Diffraction limited imaging at visible wavelengths with large ground-based telescopes

    NASA Astrophysics Data System (ADS)

    Crass, Jonathan; Mackay, Craig; King, David; Rebolo-López, Rafael; Labadie, Lucas; Puga, Marta; Oscoz, Alejandro; González Escalera, Victor; Pérez Garrido, Antonio; López, Roberto; Pérez-Prieto, Jorge; Rodríguez-Ramos, Luis; Velasco, Sergio; Villó, Isidro

    2015-01-01

    One of the continuing challenges facing astronomers today is the need to obtain ever higher resolution images of the sky. Whether studying nearby crowded fields or distant objects, with increased resolution comes the ability to probe systems in more detail and advance our understanding of the Universe. Obtaining these high-resolution images at visible wavelengths however has previously been limited to the Hubble Space Telescope (HST) due to atmospheric effects limiting the spatial resolution of ground-based telescopes to a fraction of their potential. With HST now having a finite lifespan, it is prudent to investigate other techniques capable of providing these kind of observations from the ground. Maintaining this capability is one of the goals of the Adaptive Optics Lucky Imager (AOLI).Achieving the highest resolutions requires the largest telescope apertures, however, this comes at the cost of increased atmospheric distortion. To overcome these atmospheric effects, there are two main techniques employed today: adaptive optics (AO) and lucky imaging. These techniques individually are unable to provide diffraction limited imaging in the visible on large ground-based telescopes; AO currently only works at infrared wavelengths while lucky imaging reduces in effectiveness on telescopes greater than 2.5 metres in diameter. The limitations of both techniques can be overcome by combing them together to provide diffraction limited imaging at visible wavelengths on the ground.The Adaptive Optics Lucky Imager is being developed as a European collaboration and combines AO and lucky imaging in a dedicated instrument for the first time. Initially for use on the 4.2 metre William Herschel Telescope, AOLI uses a low-order adaptive optics system to reduce the effects of atmospheric turbulence before imaging with a lucky imaging based science detector. The AO system employs a novel type of wavefront sensor, the non-linear Curvature Wavefront Sensor (nlCWFS) which provides

  12. Length-adaptive graph search for automatic segmentation of pathological features in optical coherence tomography images

    NASA Astrophysics Data System (ADS)

    Keller, Brenton; Cunefare, David; Grewal, Dilraj S.; Mahmoud, Tamer H.; Izatt, Joseph A.; Farsiu, Sina

    2016-07-01

    We introduce a metric in graph search and demonstrate its application for segmenting retinal optical coherence tomography (OCT) images of macular pathology. Our proposed "adjusted mean arc length" (AMAL) metric is an adaptation of the lowest mean arc length search technique for automated OCT segmentation. We compare this method to Dijkstra's shortest path algorithm, which we utilized previously in our popular graph theory and dynamic programming segmentation technique. As an illustrative example, we show that AMAL-based length-adaptive segmentation outperforms the shortest path in delineating the retina/vitreous boundary of patients with full-thickness macular holes when compared with expert manual grading.

  13. High-Contrast Imaging using Adaptive Optics for Extrasolar Planet Detection

    SciTech Connect

    Evans, Julia Wilhelmsen

    2006-01-01

    Direct imaging of extrasolar planets is an important, but challenging, next step in planetary science. Most planets identified to date have been detected indirectly--not by emitted or reflected light but through the effect of the planet on the parent star. For example, radial velocity techniques measure the doppler shift in the spectrum of the star produced by the presence of a planet. Indirect techniques only probe about 15% of the orbital parameter space of our solar system. Direct methods would probe new parameter space, and the detected light can be analyzed spectroscopically, providing new information about detected planets. High contrast adaptive optics systems, also known as Extreme Adaptive Optics (ExAO), will require contrasts of between 10-6 and 10-7 at angles of 4-24 λ/D on an 8-m class telescope to image young Jupiter-like planets still warm with the heat of formation. Contrast is defined as the intensity ratio of the dark wings of the image, where a planet might be, to the bright core of the star. Such instruments will be technically challenging, requiring high order adaptive optics with > 2000 actuators and improved diffraction suppression. Contrast is ultimately limited by residual static wavefront errors, so an extrasolar planet imager will require wavefront control with an accuracy of better than 1 nm rms within the low- to mid-spatial frequency range. Laboratory demonstrations are critical to instrument development. The ExAO testbed at the Laboratory for Adaptive Optics was designed with low wavefront error and precision optical metrology, which is used to explore contrast limits and develop the technology needed for an extrasolar planet imager. A state-of-the-art, 1024-actuator micro-electrical-mechanical-systems (MEMS) deformable mirror was installed and characterized to provide active wavefront control and test this novel technology. I present 6.5 x 10-8 contrast measurements with a prolate shaped pupil and

  14. High-resolution in-depth imaging of optically cleared thick samples using an adaptive SPIM

    PubMed Central

    Masson, Aurore; Escande, Paul; Frongia, Céline; Clouvel, Grégory; Ducommun, Bernard; Lorenzo, Corinne

    2015-01-01

    Today, Light Sheet Fluorescence Microscopy (LSFM) makes it possible to image fluorescent samples through depths of several hundreds of microns. However, LSFM also suffers from scattering, absorption and optical aberrations. Spatial variations in the refractive index inside the samples cause major changes to the light path resulting in loss of signal and contrast in the deepest regions, thus impairing in-depth imaging capability. These effects are particularly marked when inhomogeneous, complex biological samples are under study. Recently, chemical treatments have been developed to render a sample transparent by homogenizing its refractive index (RI), consequently enabling a reduction of scattering phenomena and a simplification of optical aberration patterns. One drawback of these methods is that the resulting RI of cleared samples does not match the working RI medium generally used for LSFM lenses. This RI mismatch leads to the presence of low-order aberrations and therefore to a significant degradation of image quality. In this paper, we introduce an original optical-chemical combined method based on an adaptive SPIM and a water-based clearing protocol enabling compensation for aberrations arising from RI mismatches induced by optical clearing methods and acquisition of high-resolution in-depth images of optically cleared complex thick samples such as Multi-Cellular Tumour Spheroids. PMID:26576666

  15. High-resolution in-depth imaging of optically cleared thick samples using an adaptive SPIM

    NASA Astrophysics Data System (ADS)

    Masson, Aurore; Escande, Paul; Frongia, Céline; Clouvel, Grégory; Ducommun, Bernard; Lorenzo, Corinne

    2015-11-01

    Today, Light Sheet Fluorescence Microscopy (LSFM) makes it possible to image fluorescent samples through depths of several hundreds of microns. However, LSFM also suffers from scattering, absorption and optical aberrations. Spatial variations in the refractive index inside the samples cause major changes to the light path resulting in loss of signal and contrast in the deepest regions, thus impairing in-depth imaging capability. These effects are particularly marked when inhomogeneous, complex biological samples are under study. Recently, chemical treatments have been developed to render a sample transparent by homogenizing its refractive index (RI), consequently enabling a reduction of scattering phenomena and a simplification of optical aberration patterns. One drawback of these methods is that the resulting RI of cleared samples does not match the working RI medium generally used for LSFM lenses. This RI mismatch leads to the presence of low-order aberrations and therefore to a significant degradation of image quality. In this paper, we introduce an original optical-chemical combined method based on an adaptive SPIM and a water-based clearing protocol enabling compensation for aberrations arising from RI mismatches induced by optical clearing methods and acquisition of high-resolution in-depth images of optically cleared complex thick samples such as Multi-Cellular Tumour Spheroids.

  16. Adaptive optics ophthalmoscopy

    PubMed Central

    Roorda, Austin; Duncan, Jacque L.

    2016-01-01

    This review starts with a brief history and description of adaptive optics (AO) technology, followed by a showcase of the latest capabilities of AO systems for imaging the human retina and an extensive review of the literature on where AO is being used clinically. The review concludes with a discussion on future directions and guidance on usage and interpretation of images from AO systems for the eye. PMID:26973867

  17. Adaptive anisotropic diffusion for noise reduction of phase images in Fourier domain Doppler optical coherence tomography.

    PubMed

    Xia, Shaoyan; Huang, Yong; Peng, Shizhao; Wu, Yanfeng; Tan, Xiaodi

    2016-08-01

    Phase image in Fourier domain Doppler optical coherence tomography offers additional flow information of investigated samples, which provides valuable evidence towards accurate medical diagnosis. High quality phase images are thus desirable. We propose a noise reduction method for phase images by combining a synthetic noise estimation criteria based on local noise estimator (LNE) and distance median value (DMV) with anisotropic diffusion model. By identifying noise and signal pixels accurately and diffusing them with different coefficients respectively and adaptive iteration steps, we demonstrated the effectiveness of our proposed method in both phantom and mouse artery images. Comparison with other methods such as filtering method (mean, median filtering), wavelet method, probabilistic method and partial differential equation based methods in terms of peak signal-to-noise ratio (PSNR), equivalent number of looks (ENL) and contrast-to-noise ratio (CNR) showed the advantages of our method in reserving image energy and removing noise. PMID:27570687

  18. Adaptive anisotropic diffusion for noise reduction of phase images in Fourier domain Doppler optical coherence tomography

    PubMed Central

    Xia, Shaoyan; Huang, Yong; Peng, Shizhao; Wu, Yanfeng; Tan, Xiaodi

    2016-01-01

    Phase image in Fourier domain Doppler optical coherence tomography offers additional flow information of investigated samples, which provides valuable evidence towards accurate medical diagnosis. High quality phase images are thus desirable. We propose a noise reduction method for phase images by combining a synthetic noise estimation criteria based on local noise estimator (LNE) and distance median value (DMV) with anisotropic diffusion model. By identifying noise and signal pixels accurately and diffusing them with different coefficients respectively and adaptive iteration steps, we demonstrated the effectiveness of our proposed method in both phantom and mouse artery images. Comparison with other methods such as filtering method (mean, median filtering), wavelet method, probabilistic method and partial differential equation based methods in terms of peak signal-to-noise ratio (PSNR), equivalent number of looks (ENL) and contrast-to-noise ratio (CNR) showed the advantages of our method in reserving image energy and removing noise. PMID:27570687

  19. Adaptive optics scanning laser ophthalmoscopy for in vivo imaging of lamina cribrosa

    NASA Astrophysics Data System (ADS)

    Vilupuru, Abhiram S.; Rangaswamy, Nalini V.; Frishman, Laura J.; Smith, Earl L., III; Harwerth, Ronald S.; Roorda, Austin

    2007-05-01

    The lamina cribrosa has been postulated from in vitro studies as an early site of damage in glaucoma. Prior in vivo measures of laminar morphology have been confounded by ocular aberrations. In this study the lamina cribrosa was imaged after correcting for ocular aberrations using the adaptive optics scanning laser ophthalmoscope (AOSLO) in normal and glaucomatous eyes of rhesus monkeys. All measured laminar morphological parameters showed increased magnitudes in glaucomatous eyes relative to fellow control eyes, indicating altered structure. The AOSLO provides high-quality images of the lamina cribrosa and may have potential as a tool for early identification of glaucoma.

  20. Image improvement from a sodium-layer laser guide star adaptive optics system

    SciTech Connect

    Max, C. E., LLNL

    1997-06-01

    A sodium-layer laser guide star beacon with high-order adaptive optics at Lick Observatory produced a factor of 2.4 intensity increase and a factor of 2 decrease in full width at half maximum for an astronomical point source, compared with image motion compensation alone. Image full widths at half maximum were identical for laser and natural guide stars (0.3 arc seconds). The Strehl ratio with the laser guide star was 65% of that with a natural guide star. This technique should allow ground-based telescopes to attain the diffraction limit, by correcting for atmospheric distortions.

  1. Robotic Transit Follow-up: Adaptive Optics Imaging of Thousands of Stars

    NASA Astrophysics Data System (ADS)

    Law, Nicholas M.; Morton, T.; Baranec, C.; Riddle, R. L.; Tendulkar, S. P.; Johnson, J. A.; Bui, K.; Burse, M.; Chordia, P.; Das, H.; Dekany, R.; Kulkarni, S. R.; Punnadi, S.; Ramaprakash, A. N.; Robo-AO Collaboration

    2013-01-01

    Stars that host transiting exoplanet candidates may have close companions. If undetected, these companions can produce false-positive planets or affect the measured exoplanet characteristics. High-angular-resolution imaging is required to resolve these systems. Up to now, it has been impossible to obtain adaptive optics images of all the thousands of candidates generated by large surveys like Kepler because of the faintness of the targets and the excessive observing time required. The Robo-AO robotic laser adaptive optics system, newly-commissioned on the Palomar 60-inch telescope, is the first system capable of rapidly observing thousands of targets at high resolution. Robo-AO routinely images 200+ targets per night and produces 0.1" FWHM images in visible wavelengths similar to the Kepler passband. We are using Robo-AO to perform a stellar companion search of unprecedented size, including every Kepler planet candidate and 3,000 nearby planet-search stars. In our first observing season we have imaged over 1,000 Kepler objects of interest and 75% of the Northern stars within 25pc. We will describe the system and discuss its use for future exoplanet surveys such as TESS. We will also present the first results from the survey: a comprehensive assessment of stellar multiplicity among Kepler exoplanet hosts and the discovery of new close stellar companions around Kepler objects of interest.

  2. Multimodal adaptive optics for depth-enhanced high-resolution ophthalmic imaging

    NASA Astrophysics Data System (ADS)

    Hammer, Daniel X.; Mujat, Mircea; Iftimia, Nicusor V.; Lue, Niyom; Ferguson, R. Daniel

    2010-02-01

    We developed a multimodal adaptive optics (AO) retinal imager for diagnosis of retinal diseases, including glaucoma, diabetic retinopathy (DR), age-related macular degeneration (AMD), and retinitis pigmentosa (RP). The development represents the first ever high performance AO system constructed that combines AO-corrected scanning laser ophthalmoscopy (SLO) and swept source Fourier domain optical coherence tomography (SSOCT) imaging modes in a single compact clinical prototype platform. The SSOCT channel operates at a wavelength of 1 μm for increased penetration and visualization of the choriocapillaris and choroid, sites of major disease activity for DR and wet AMD. The system is designed to operate on a broad clinical population with a dual deformable mirror (DM) configuration that allows simultaneous low- and high-order aberration correction. The system also includes a wide field line scanning ophthalmoscope (LSO) for initial screening, target identification, and global orientation; an integrated retinal tracker (RT) to stabilize the SLO, OCT, and LSO imaging fields in the presence of rotational eye motion; and a high-resolution LCD-based fixation target for presentation to the subject of stimuli and other visual cues. The system was tested in a limited number of human subjects without retinal disease for performance optimization and validation. The system was able to resolve and quantify cone photoreceptors across the macula to within ~0.5 deg (~100-150 μm) of the fovea, image and delineate ten retinal layers, and penetrate to resolve targets deep into the choroid. In addition to instrument hardware development, analysis algorithms were developed for efficient information extraction from clinical imaging sessions, with functionality including automated image registration, photoreceptor counting, strip and montage stitching, and segmentation. The system provides clinicians and researchers with high-resolution, high performance adaptive optics imaging to help

  3. Multiphoton imaging microscopy at deeper layers with adaptive optics control of spherical aberration.

    PubMed

    Bueno, Juan M; Skorsetz, Martin; Palacios, Raquel; Gualda, Emilio J; Artal, Pablo

    2014-01-01

    Despite the inherent confocality and optical sectioning capabilities of multiphoton microscopy, three-dimensional (3-D) imaging of thick samples is limited by the specimen-induced aberrations. The combination of immersion objectives and sensorless adaptive optics (AO) techniques has been suggested to overcome this difficulty. However, a complex plane-by-plane correction of aberrations is required, and its performance depends on a set of image-based merit functions. We propose here an alternative approach to increase penetration depth in 3-D multiphoton microscopy imaging. It is based on the manipulation of the spherical aberration (SA) of the incident beam with an AO device while performing fast tomographic multiphoton imaging. When inducing SA, the image quality at best focus is reduced; however, better quality images are obtained from deeper planes within the sample. This is a compromise that enables registration of improved 3-D multiphoton images using nonimmersion objectives. Examples on ocular tissues and nonbiological samples providing different types of nonlinear signal are presented. The implementation of this technique in a future clinical instrument might provide a better visualization of corneal structures in living eyes.

  4. Image Tracking of Multiple C. Elegans Worms Using Adaptive Scanning Optical Microscope (ASOM)

    NASA Astrophysics Data System (ADS)

    Rivera, Linda; Potsaid, Benjamin; Wen, John T.

    2010-03-01

    Long-term imaging of living biological specimens is important to infer behaviorial trends and correlate neural structure with behavior. Such study is plagued by the field of view limitation in standard optical microscopes, as the motile specimen would frequently move out of view. A novel microscope, called the adaptive scanning optical microscope (ASOM), has recently been proposed to address this limitation. Through high speed post-objective scanning with a steering mirror, and compensation for optical aberrations with a MEMS deformable mirror, simultaneous imaging and tracking of multiple Caenorhabditis elegans worms has been demonstrated. This article presents the image processing algorithm for tracking multiple worms. Since the steering mirror has to move based on the predicted worm motion, image processing and stable steering mirror motion need to be executed at higher than the composite mosaic video frame rate (in contrast to existing works on image-based worm tracking, which are predominantly based on post-processing). Particular care is placed on disambiguating the worms when they overlap, collide, or entangle, where the worm tracking algorithm may fail. Results from both real time and simulated tracking are presented.

  5. High-resolution retinal imaging through open-loop adaptive optics

    NASA Astrophysics Data System (ADS)

    Li, Chao; Xia, Mingliang; Li, Dayu; Mu, Quanquan; Xuan, Li

    2010-07-01

    Using the liquid crystal spatial light modulator (LC-SLM) as the wavefront corrector, an open-loop adaptive optics (AO) system for fundus imaging in vivo is constructed. Compared with the LC-SLM closed-loop AO system, the light energy efficiency is increased by a factor of 2, which is helpful for the safety of fundus illumination in vivo. In our experiment, the subjective accommodation method is used to precorrect the defocus aberration, and three subjects with different myopia 0, -3, and -5 D are tested. Although the residual wavefront error after correction cannot to detected, the fundus images adequately demonstrate that the imaging system reaches the resolution of a single photoreceptor cell through the open-loop correction. Without dilating and cyclopleging the eye, the continuous imaging for 8 s is recorded for one of the subjects.

  6. Adaptive Sensor Optimization and Cognitive Image Processing Using Autonomous Optical Neuroprocessors

    SciTech Connect

    CAMERON, STEWART M.

    2001-10-01

    Measurement and signal intelligence demands has created new requirements for information management and interoperability as they affect surveillance and situational awareness. Integration of on-board autonomous learning and adaptive control structures within a remote sensing platform architecture would substantially improve the utility of intelligence collection by facilitating real-time optimization of measurement parameters for variable field conditions. A problem faced by conventional digital implementations of intelligent systems is the conflict between a distributed parallel structure on a sequential serial interface functionally degrading bandwidth and response time. In contrast, optically designed networks exhibit the massive parallelism and interconnect density needed to perform complex cognitive functions within a dynamic asynchronous environment. Recently, all-optical self-organizing neural networks exhibiting emergent collective behavior which mimic perception, recognition, association, and contemplative learning have been realized using photorefractive holography in combination with sensory systems for feature maps, threshold decomposition, image enhancement, and nonlinear matched filters. Such hybrid information processors depart from the classical computational paradigm based on analytic rules-based algorithms and instead utilize unsupervised generalization and perceptron-like exploratory or improvisational behaviors to evolve toward optimized solutions. These systems are robust to instrumental systematics or corrupting noise and can enrich knowledge structures by allowing competition between multiple hypotheses. This property enables them to rapidly adapt or self-compensate for dynamic or imprecise conditions which would be unstable using conventional linear control models. By incorporating an intelligent optical neuroprocessor in the back plane of an imaging sensor, a broad class of high-level cognitive image analysis problems including geometric

  7. Phase sensitive adaptive optics assisted SLO/OCT for retinal imaging (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Pircher, Michael; Felberer, Franz; Salas, Matthias; Haindl, Richard; Baumann, Bernhard; Wartak, Andreas; Hitzenberger, Christoph K.

    2016-03-01

    Adaptive optics (AO) is essential in order to visualize small structures such as cone and rod photoreceptors in the living human retina in vivo. By combining AO with optical coherence tomography (OCT) the axial resolution in the images can be further improved. OCT provides access to the phase of the light returning from the retina which allows a measurement of subtle length changes in the nanometer range. These occur for example during the renewal process of cone outer segments. We present an approach for measuring very small length changes using an extended AO scanning laser ophthalmoscope (SLO)/ OCT instrument. By adding a second OCT interferometer that shares the same sample arm as the first interferometer, phase sensitive measurements can be performed in the en-face imaging plane. Frame averaging decreases phase noise which greatly improves the precision in the measurement of associated length changes.

  8. Automatic detection of cone photoreceptors in split detector adaptive optics scanning light ophthalmoscope images.

    PubMed

    Cunefare, David; Cooper, Robert F; Higgins, Brian; Katz, David F; Dubra, Alfredo; Carroll, Joseph; Farsiu, Sina

    2016-05-01

    Quantitative analysis of the cone photoreceptor mosaic in the living retina is potentially useful for early diagnosis and prognosis of many ocular diseases. Non-confocal split detector based adaptive optics scanning light ophthalmoscope (AOSLO) imaging reveals the cone photoreceptor inner segment mosaics often not visualized on confocal AOSLO imaging. Despite recent advances in automated cone segmentation algorithms for confocal AOSLO imagery, quantitative analysis of split detector AOSLO images is currently a time-consuming manual process. In this paper, we present the fully automatic adaptive filtering and local detection (AFLD) method for detecting cones in split detector AOSLO images. We validated our algorithm on 80 images from 10 subjects, showing an overall mean Dice's coefficient of 0.95 (standard deviation 0.03), when comparing our AFLD algorithm to an expert grader. This is comparable to the inter-observer Dice's coefficient of 0.94 (standard deviation 0.04). To the best of our knowledge, this is the first validated, fully-automated segmentation method which has been applied to split detector AOSLO images.

  9. Automatic detection of cone photoreceptors in split detector adaptive optics scanning light ophthalmoscope images

    PubMed Central

    Cunefare, David; Cooper, Robert F.; Higgins, Brian; Katz, David F.; Dubra, Alfredo; Carroll, Joseph; Farsiu, Sina

    2016-01-01

    Quantitative analysis of the cone photoreceptor mosaic in the living retina is potentially useful for early diagnosis and prognosis of many ocular diseases. Non-confocal split detector based adaptive optics scanning light ophthalmoscope (AOSLO) imaging reveals the cone photoreceptor inner segment mosaics often not visualized on confocal AOSLO imaging. Despite recent advances in automated cone segmentation algorithms for confocal AOSLO imagery, quantitative analysis of split detector AOSLO images is currently a time-consuming manual process. In this paper, we present the fully automatic adaptive filtering and local detection (AFLD) method for detecting cones in split detector AOSLO images. We validated our algorithm on 80 images from 10 subjects, showing an overall mean Dice’s coefficient of 0.95 (standard deviation 0.03), when comparing our AFLD algorithm to an expert grader. This is comparable to the inter-observer Dice’s coefficient of 0.94 (standard deviation 0.04). To the best of our knowledge, this is the first validated, fully-automated segmentation method which has been applied to split detector AOSLO images. PMID:27231641

  10. Interpretation of Flood-Illuminated Adaptive Optics Images in Subjects with Retinitis Pigmentosa.

    PubMed

    Gale, Michael J; Feng, Shu; Titus, Hope E; Smith, Travis B; Pennesi, Mark E

    2016-01-01

    The purpose of this study was to correlate features on flood-illuminated adaptive optics (AO) images with color fundus, fundus autofluorescence (FAF) and spectral domain optical coherence tomography (SD-OCT) images in patients with retinitis pigmentosa (RP). We imaged 39 subjects diagnosed with RP using the rtx1™ flood-illuminated AO camera from Imagine Eyes (Orsay, France). We observed a correlation between hyper-autofluoresence changes on FAF, disruption of the interdigitation zone (IZ) on SD-OCT and loss of reflective cone profiles on AO. Four main patterns of cone-reflectivity were seen on AO: presumed healthy cone mosaics, hypo-reflective blurred cone-like structures, higher frequency disorganized hyper-reflective spots, and lower frequency hypo-reflective spots. These regions were correlated to progressive phases of cone photoreceptor degeneration observed using SD-OCT and FAF. These results help provide interpretation of en face images obtained by flood-illuminated AO in subjects with RP. However, significant ambiguity remains as to what truly constitutes a cone, especially in areas of degeneration. With further refinements in technology, flood illuminated AO imaging has the potential to provide rapid, standardized, longitudinal and lower cost imaging in patients with retinal degeneration.

  11. Adaptive wiener image restoration kernel

    DOEpatents

    Yuan, Ding

    2007-06-05

    A method and device for restoration of electro-optical image data using an adaptive Wiener filter begins with constructing imaging system Optical Transfer Function, and the Fourier Transformations of the noise and the image. A spatial representation of the imaged object is restored by spatial convolution of the image using a Wiener restoration kernel.

  12. MAD Adaptive Optics Imaging of High-luminosity Quasars: A Pilot Project

    NASA Astrophysics Data System (ADS)

    Liuzzo, E.; Falomo, R.; Paiano, S.; Treves, A.; Uslenghi, M.; Arcidiacono, C.; Baruffolo, A.; Diolaiti, E.; Farinato, J.; Lombini, M.; Moretti, A.; Ragazzoni, R.; Brast, R.; Donaldson, R.; Kolb, J.; Marchetti, E.; Tordo, S.

    2016-08-01

    We present near-IR images of five luminous quasars at z ˜ 2 and one at z ˜ 4 obtained with an experimental adaptive optics (AO) instrument at the European Southern Observatory Very Large Telescope. The observations are part of a program aimed at demonstrating the capabilities of multi-conjugated adaptive optics imaging combined with the use of natural guide stars for high spatial resolution studies on large telescopes. The observations were mostly obtained under poor seeing conditions but in two cases. In spite of these nonoptimal conditions, the resulting images of point sources have cores of FWHM ˜ 0.2 arcsec. We are able to characterize the host galaxy properties for two sources and set stringent upper limits to the galaxy luminosity for the others. We also report on the expected capabilities for investigating the host galaxies of distant quasars with AO systems coupled with future Extremely Large Telescopes. Detailed simulations show that it will be possible to characterize compact (2-3 kpc) quasar host galaxies for quasi-stellar objects at z = 2 with nucleus K-magnitude spanning from 15 to 20 (corresponding to absolute magnitude -31 to -26) and host galaxies that are 4 mag fainter than their nuclei.

  13. High Resolution Imaging with Adaptive Optics at the Multiple Mirror Telescope

    NASA Astrophysics Data System (ADS)

    Lloyd-Hart, M.; McLeod, B. A.; Wittman, D.; Colucci, D.; McCarthy, D. W.; Angel, R.; Dekany, R.

    1992-12-01

    We present the latest results from an adaptive optics program being implemented at the MMT using a six element adaptive mirror. The tilt of the wavefront over each of the six telescopes is determined with a Shack-Hartmann type sensor using a 24times 24 pixel low-noise CCD. This system allows the MMT to operate at a resolution of 0.3'' at 2 microns -- near the diffraction limit of the individual 1.8-m telescopes. This resolution can be obtained within ~ 1' of any star with visual magnitude < 16, allowing high-resolution near-IR imaging with a NICMOS2 array of a wide variety of targets, including high-redshift galaxies and young and evolved stars. This system can also be used with the MMT operated as a phased array telescope. In this mode, the piston errors between the telescopes are determined by examining the Fourier transform of the combined 2-micron image of the natural guide star using a fast-readout InSb array. In this configuration we have achieved a resolution of 0.075''. In good seeing we expect to obtain images of interest within the isoplanatic patch of guide stars with K magnitude <7. We acknowledge financial support from the NSF (AST92-03336) and the Flintridge Foundation. The adaptive mirror was donated by ThermoTrex Corp.

  14. Comparison of adaptive optics scanning light ophthalmoscopic fluorescein angiography and offset pinhole imaging.

    PubMed

    Chui, Toco Y P; Dubow, Michael; Pinhas, Alexander; Shah, Nishit; Gan, Alexander; Weitz, Rishard; Sulai, Yusufu N; Dubra, Alfredo; Rosen, Richard B

    2014-04-01

    Recent advances to the adaptive optics scanning light ophthalmoscope (AOSLO) have enabled finer in vivo assessment of the human retinal microvasculature. AOSLO confocal reflectance imaging has been coupled with oral fluorescein angiography (FA), enabling simultaneous acquisition of structural and perfusion images. AOSLO offset pinhole (OP) imaging combined with motion contrast post-processing techniques, are able to create a similar set of structural and perfusion images without the use of exogenous contrast agent. In this study, we evaluate the similarities and differences of the structural and perfusion images obtained by either method, in healthy control subjects and in patients with retinal vasculopathy including hypertensive retinopathy, diabetic retinopathy, and retinal vein occlusion. Our results show that AOSLO OP motion contrast provides perfusion maps comparable to those obtained with AOSLO FA, while AOSLO OP reflectance images provide additional information such as vessel wall fine structure not as readily visible in AOSLO confocal reflectance images. AOSLO OP offers a non-invasive alternative to AOSLO FA without the need for any exogenous contrast agent.

  15. Laser guide star adaptive optics imaging polarimetry of Herbig Ae/Be stars.

    PubMed

    Perrin, Marshall D; Graham, James R; Kalas, Paul; Lloyd, James P; Max, Claire E; Gavel, Donald T; Pennington, Deanna M; Gates, Elinor L

    2004-02-27

    We have used laser guide star adaptive optics and a near-infrared dual-channel imaging polarimeter to observe light scattered in the circumstellar environment of Herbig Ae/Be stars on scales of 100 to 300 astronomical units. We revealed a strongly polarized, biconical nebula 10 arc seconds (6000 astronomical units) in diameter around the star LkHalpha 198 and also observed a polarized jet-like feature associated with the deeply embedded source LkHalpha 198-IR. The star LkHalpha 233 presents a narrow, unpolarized dark lane consistent with an optically thick circumstellar disk blocking our direct view of the star. These data show that the lower-mass T Tauri and intermediate mass Herbig Ae/Be stars share a common evolutionary sequence.

  16. Strategies to overcome photobleaching in algorithm-based adaptive optics for nonlinear in-vivo imaging.

    PubMed

    Caroline Müllenbroich, M; McGhee, Ewan J; Wright, Amanda J; Anderson, Kurt I; Mathieson, Keith

    2014-01-01

    We have developed a nonlinear adaptive optics microscope utilizing a deformable membrane mirror (DMM) and demonstrated its use in compensating for system- and sample-induced aberrations. The optimum shape of the DMM was determined with a random search algorithm optimizing on either two photon fluorescence or second harmonic signals as merit factors. We present here several strategies to overcome photobleaching issues associated with lengthy optimization routines by adapting the search algorithm and the experimental methodology. Optimizations were performed on extrinsic fluorescent dyes, fluorescent beads loaded into organotypic tissue cultures and the intrinsic second harmonic signal of these cultures. We validate the approach of using these preoptimized mirror shapes to compile a robust look-up table that can be applied for imaging over several days and through a variety of tissues. In this way, the photon exposure to the fluorescent cells under investigation is limited to imaging. Using our look-up table approach, we show signal intensity improvement factors ranging from 1.7 to 4.1 in organotypic tissue cultures and freshly excised mouse tissue. Imaging zebrafish in vivo, we demonstrate signal improvement by a factor of 2. This methodology is easily reproducible and could be applied to many photon starved experiments, for example fluorescent life time imaging, or when photobleaching is a concern.

  17. Imaging microscopic structures in pathological retinas using a flood-illumination adaptive optics retinal camera

    NASA Astrophysics Data System (ADS)

    Viard, Clément; Nakashima, Kiyoko; Lamory, Barbara; Pâques, Michel; Levecq, Xavier; Château, Nicolas

    2011-03-01

    This research is aimed at characterizing in vivo differences between healthy and pathological retinal tissues at the microscopic scale using a compact adaptive optics (AO) retinal camera. Tests were performed in 120 healthy eyes and 180 eyes suffering from 19 different pathological conditions, including age-related maculopathy (ARM), glaucoma and rare diseases such as inherited retinal dystrophies. Each patient was first examined using SD-OCT and infrared SLO. Retinal areas of 4°x4° were imaged using an AO flood-illumination retinal camera based on a large-stroke deformable mirror. Contrast was finally enhanced by registering and averaging rough images using classical algorithms. Cellular-resolution images could be obtained in most cases. In ARM, AO images revealed granular contents in drusen, which were invisible in SLO or OCT images, and allowed the observation of the cone mosaic between drusen. In glaucoma cases, visual field was correlated to changes in cone visibility. In inherited retinal dystrophies, AO helped to evaluate cone loss across the retina. Other microstructures, slightly larger in size than cones, were also visible in several retinas. AO provided potentially useful diagnostic and prognostic information in various diseases. In addition to cones, other microscopic structures revealed by AO images may also be of interest in monitoring retinal diseases.

  18. Ultra-high resolution adaptive optics: optical coherence tomography for in vivo imaging of healthy and diseased retinal structures

    NASA Astrophysics Data System (ADS)

    Zawadzki, Robert J.; Zhang, Yan; Jones, Steven M.; Choi, Stacey S.; Cense, Barry; Evans, Julia W.; Miller, Donald T.; Olivier, Scot S.; Werner, John S.

    2008-02-01

    Ultra-high isotropic resolution imaging of retinal structures was made possible with an adaptive optics system using dual deformable mirrors and a Fourier-domain optical coherence tomography (Fd-OCT) system with correction for longitudinal chromatic aberration. This system was used to image microscopic retinal structures of healthy as well as diseased retinas in vivo. The improved resolution and contrast enhanced visualization of morphological structures in the retina can be clearly seen. The benefits of this instrument are apparent from comparison of new images with those acquired using a previous generation AO-OCT instrument. Big change in the appearance of speckle field (reduction in speckle size) can be observed as well. Additionally, further improvements in volumetric data acquisition and image representation will be discussed. This includes creation of large Field of View (FOV) AO-OCT volume from multiple sub-volumes and its visualization. Also techniques and results of reducing speckle contrast by averaging multiple B-scans will be presented.

  19. SHARP - iii. First Use of Adaptive Optics Imaging to Constrain Cosmology with Gravitational Lens Time Delays

    NASA Astrophysics Data System (ADS)

    Chen, Geoff C. F.; Suyu, Sherry H.; Wong, Kenneth C.; Fassnacht, Christopher D.; Chiueh, Tzihong; Halkola, Aleksi; Hu, I. Shing; Auger, Matthew W.; Koopmans, Léon V. E.; Lagattuta, David J.; McKean, John P.; Vegetti, Simona

    2016-08-01

    Accurate and precise measurements of the Hubble constant are critical for testing our current standard cosmological model and revealing possibly new physics. With Hubble Space Telescope (HST) imaging, each strong gravitational lens system with measured time delays can allow one to determine the Hubble constant with an uncertainty of ˜7%. Since HST will not last forever, we explore adaptive-optics (AO) imaging as an alternative that can provide higher angular resolution than HST imaging but has a less stable point spread function (PSF) due to atmospheric distortion. To make AO imaging useful for time-delay-lens cosmography, we develop a method to extract the unknown PSF directly from the imaging of strongly lensed quasars. In a blind test with two mock data sets created with different PSFs, we are able to recover the important cosmological parameters (time-delay distance, external shear, lens mass profile slope, and total Einstein radius). Our analysis of the Keck AO image of the strong lens system RXJ 1131-1231 shows that the important parameters for cosmography agree with those based on HST imaging and modeling within 1-σ uncertainties. Most importantly, the constraint on the model time-delay distance by using AO imaging with 0.045″ resolution is tighter by ˜50% than the constraint of time-delay distance by using HST imaging with 0.09″ when a power-law mass distribution for the lens system is adopted. Our PSF reconstruction technique is generic and applicable to data sets that have multiple nearby point sources, enabling scientific studies that require high-precision models of the PSF.

  20. Dynamically adaptive mesh refinement technique for image reconstruction in optical tomography.

    PubMed

    Soloviev, Vadim Y; Krasnosselskaia, Lada V

    2006-04-20

    A novel adaptive mesh technique is introduced for problems of image reconstruction in luminescence optical tomography. A dynamical adaptation of the three-dimensional scheme based on the finite-volume formulation reduces computational time and balances the ill-posed nature of the inverse problem. The arbitrary shape of the bounding surface is handled by an additional refinement of computational cells on the boundary. Dynamical shrinking of the search volume is introduced to improve computational performance and accuracy while locating the luminescence target. Light propagation in the medium is modeled by the telegraph equation, and the image-reconstruction algorithm is derived from the Fredholm integral equation of the first kind. Stability and computational efficiency of the introduced method are demonstrated for image reconstruction of one and two spherical luminescent objects embedded within a breastlike tissue phantom. Experimental measurements are simulated by the solution of the forward problem on a grid of 5x5 light guides attached to the surface of the phantom.

  1. High Redshift Dust Obscured Galaxies, A Morphology-SED Connection Revealed by Keck Adaptive Optics Imaging

    NASA Astrophysics Data System (ADS)

    Melbourne, Jason

    2009-01-01

    Keck Adaptive Optics (AO) K'-band images reveal the morphologies of 15 high redshift (z 2) dust obscured galaxies (DOGs). DOGs are defined by an optical to mid-IR color of fν(24) / fν(R) > 1000, redder than Arp 220 at any redshift. With ultra-luminous infrared luminosities, DOGs are thought to be powered by a combination of AGN and star formation. We use high spatial resolution (0.5 - 1 kpc at these redshifts) AO images to help disentangle the dominant energy source in each DOG and to look for triggers, such as evidence of ongoing mergers. We find evidence for ongoing merging in 10-20% of the sample. We also find a statistically significant correlation between galaxy compactness and 24 micron flux (luminosity), with the brightest DOGs exhibiting more compact morphologies than fainter DOGs. The most diffuse systems tend to show a 1.6 micron stellar bump in their spectral energy distributions redshifted to the Spitzer IRAC bands (4.5 - 8.0 microns). The imaging results lend further support to the idea that the highest luminosity DOGs are AGN dominated (resulting in compact morphology), while the lower luminosity, diffuse, DOGs tend to be star formation dominated.

  2. Optimization of the open-loop liquid crystal adaptive optics retinal imaging system

    NASA Astrophysics Data System (ADS)

    Kong, Ningning; Li, Chao; Xia, Mingliang; Li, Dayu; Qi, Yue; Xuan, Li

    2012-02-01

    An open-loop adaptive optics (AO) system for retinal imaging was constructed using a liquid crystal spatial light modulator (LC-SLM) as the wavefront compensator. Due to the dispersion of the LC-SLM, there was only one illumination source for both aberration detection and retinal imaging in this system. To increase the field of view (FOV) for retinal imaging, a modified mechanical shutter was integrated into the illumination channel to control the size of the illumination spot on the fundus. The AO loop was operated in a pulsing mode, and the fundus was illuminated twice by two laser impulses in a single AO correction loop. As a result, the FOV for retinal imaging was increased to 1.7-deg without compromising the aberration detection accuracy. The correction precision of the open-loop AO system was evaluated in a closed-loop configuration; the residual error is approximately 0.0909λ (root-mean-square, RMS), and the Strehl ratio ranges to 0.7217. Two subjects with differing rates of myopia (-3D and -5D) were tested. High-resolution images of capillaries and photoreceptors were obtained.

  3. 3D imaging of cone photoreceptors over extended time periods using optical coherence tomography with adaptive optics

    NASA Astrophysics Data System (ADS)

    Kocaoglu, Omer P.; Lee, Sangyeol; Jonnal, Ravi S.; Wang, Qiang; Herde, Ashley E.; Besecker, Jason; Gao, Weihua; Miller, Donald T.

    2011-03-01

    Optical coherence tomography with adaptive optics (AO-OCT) is a highly sensitive, noninvasive method for 3D imaging of the microscopic retina. The purpose of this study is to advance AO-OCT technology by enabling repeated imaging of cone photoreceptors over extended periods of time (days). This sort of longitudinal imaging permits monitoring of 3D cone dynamics in both normal and diseased eyes, in particular the physiological processes of disc renewal and phagocytosis, which are disrupted by retinal diseases such as age related macular degeneration and retinitis pigmentosa. For this study, the existing AO-OCT system at Indiana underwent several major hardware and software improvements to optimize system performance for 4D cone imaging. First, ultrahigh speed imaging was realized using a Basler Sprint camera. Second, a light source with adjustable spectrum was realized by integration of an Integral laser (Femto Lasers, λc=800nm, ▵λ=160nm) and spectral filters in the source arm. For cone imaging, we used a bandpass filter with λc=809nm and ▵λ=81nm (2.6 μm nominal axial resolution in tissue, and 167 KHz A-line rate using 1,408 px), which reduced the impact of eye motion compared to previous AO-OCT implementations. Third, eye motion artifacts were further reduced by custom ImageJ plugins that registered (axially and laterally) the volume videos. In two subjects, cone photoreceptors were imaged and tracked over a ten day period and their reflectance and outer segment (OS) lengths measured. High-speed imaging and image registration/dewarping were found to reduce eye motion to a fraction of a cone width (1 μm root mean square). The pattern of reflections in the cones was found to change dramatically and occurred on a spatial scale well below the resolution of clinical instruments. Normalized reflectance of connecting cilia (CC) and OS posterior tip (PT) of an exemplary cone was 54+/-4, 47+/-4, 48+/-6, 50+/-5, 56+/-1% and 46+/-4, 53+/-4, 52+/-6, 50+/-5, 44

  4. Technical Factors Influencing Cone Packing Density Estimates in Adaptive Optics Flood Illuminated Retinal Images

    PubMed Central

    Lombardo, Marco; Serrao, Sebastiano; Lombardo, Giuseppe

    2014-01-01

    Purpose To investigate the influence of various technical factors on the variation of cone packing density estimates in adaptive optics flood illuminated retinal images. Methods Adaptive optics images of the photoreceptor mosaic were obtained in fifteen healthy subjects. The cone density and Voronoi diagrams were assessed in sampling windows of 320×320 µm, 160×160 µm and 64×64 µm at 1.5 degree temporal and superior eccentricity from the preferred locus of fixation (PRL). The technical factors that have been analyzed included the sampling window size, the corrected retinal magnification factor (RMFcorr), the conversion from radial to linear distance from the PRL, the displacement between the PRL and foveal center and the manual checking of cone identification algorithm. Bland-Altman analysis was used to assess the agreement between cone density estimated within the different sampling window conditions. Results The cone density declined with decreasing sampling area and data between areas of different size showed low agreement. A high agreement was found between sampling areas of the same size when comparing density calculated with or without using individual RMFcorr. The agreement between cone density measured at radial and linear distances from the PRL and between data referred to the PRL or the foveal center was moderate. The percentage of Voronoi tiles with hexagonal packing arrangement was comparable between sampling areas of different size. The boundary effect, presence of any retinal vessels, and the manual selection of cones missed by the automated identification algorithm were identified as the factors influencing variation of cone packing arrangements in Voronoi diagrams. Conclusions The sampling window size is the main technical factor that influences variation of cone density. Clear identification of each cone in the image and the use of a large buffer zone are necessary to minimize factors influencing variation of Voronoi diagrams of the cone

  5. In Vivo Imaging of the Human Retinal Pigment Epithelial Mosaic Using Adaptive Optics Enhanced Indocyanine Green Ophthalmoscopy

    PubMed Central

    Tam, Johnny; Liu, Jianfei; Dubra, Alfredo; Fariss, Robert

    2016-01-01

    Purpose The purpose of this study was to establish that retinal pigment epithelial (RPE) cells take up indocyanine green (ICG) dye following systemic injection and that adaptive optics enhanced indocyanine green ophthalmoscopy (AO-ICG) enables direct visualization of the RPE mosaic in the living human eye. Methods A customized adaptive optics scanning light ophthalmoscope (AOSLO) was used to acquire high-resolution retinal fluorescence images of residual ICG dye in human subjects after intravenous injection at the standard clinical dose. Simultaneously, multimodal AOSLO images were also acquired, which included confocal reflectance, nonconfocal split detection, and darkfield. Imaging was performed in 6 eyes of three healthy subjects with no history of ocular or systemic diseases. In addition, histologic studies in mice were carried out. Results The AO-ICG channel successfully resolved individual RPE cells in human subjects at various time points, including 20 minutes and 2 hours after dye administration. Adaptive optics-ICG images of RPE revealed detail which could be correlated with AO dark-field images of the same cells. Interestingly, there was a marked heterogeneity in the fluorescence of individual RPE cells. Confirmatory histologic studies in mice corroborated the specific uptake of ICG by the RPE layer at a late time point after systemic ICG injection. Conclusions Adaptive optics-enhanced imaging of ICG dye provides a novel way to visualize and assess the RPE mosaic in the living human eye alongside images of the overlying photoreceptors and other cells. PMID:27564519

  6. The Robo-AO KOI Survey: Laser Adaptive Optics Imaging of Every Kepler Exoplanet Candidate

    NASA Astrophysics Data System (ADS)

    Ziegler, Carl; Law, Nicholas M.; Baranec, Christoph; Morton, Tim; Riddle, Reed L.

    2016-01-01

    The Robo-AO Kepler Planetary Candidate Survey is observing every Kepler planet candidate host star (KOI) with laser adaptive optics imaging to hunt for blended nearby stars which may be physically associated companions. With the unparalleled efficiency provided by the first fully robotic adaptive optics system, we perform the critical search for nearby stars (0.15" to 4.0" separation with contrasts up to 6 magnitudes) that pollute the observed planetary transit signal, contributing to inaccurate planetary characteristics or astrophysical false positives. We present approximately 3300 high resolution observations of Kepler planetary hosts from 2012-2015, with ~500 observed nearby stars. We measure an overall nearby star probability rate of 16.2±0.8%. With this large dataset, we are uniquely able to explore broad correlations between multiple star systems and the properties of the planets which they host. We then use these clues for insight into the formation and evolution of these exotic systems. Several KOIs of particular interest will be discussed, including possible quadruple star systems hosting planets and updated properties for possible rocky planets orbiting in the habitable zone.

  7. Automated segmentation of retinal pigment epithelium cells in fluorescence adaptive optics images.

    PubMed

    Rangel-Fonseca, Piero; Gómez-Vieyra, Armando; Malacara-Hernández, Daniel; Wilson, Mario C; Williams, David R; Rossi, Ethan A

    2013-12-01

    Adaptive optics (AO) imaging methods allow the histological characteristics of retinal cell mosaics, such as photoreceptors and retinal pigment epithelium (RPE) cells, to be studied in vivo. The high-resolution images obtained with ophthalmic AO imaging devices are rich with information that is difficult and/or tedious to quantify using manual methods. Thus, robust, automated analysis tools that can provide reproducible quantitative information about the cellular mosaics under examination are required. Automated algorithms have been developed to detect the position of individual photoreceptor cells; however, most of these methods are not well suited for characterizing the RPE mosaic. We have developed an algorithm for RPE cell segmentation and show its performance here on simulated and real fluorescence AO images of the RPE mosaic. Algorithm performance was compared to manual cell identification and yielded better than 91% correspondence. This method can be used to segment RPE cells for morphometric analysis of the RPE mosaic and speed the analysis of both healthy and diseased RPE mosaics.

  8. Eye-pupil displacement and prediction: effects on residual wavefront in adaptive optics retinal imaging.

    PubMed

    Kulcsár, Caroline; Raynaud, Henri-François; Garcia-Rissmann, Aurea

    2016-03-01

    This paper studies the effect of pupil displacements on the best achievable performance of retinal imaging adaptive optics (AO) systems, using 52 trajectories of horizontal and vertical displacements sampled at 80 Hz by a pupil tracker (PT) device on 13 different subjects. This effect is quantified in the form of minimal root mean square (rms) of the residual phase affecting image formation, as a function of the delay between PT measurement and wavefront correction. It is shown that simple dynamic models identified from data can be used to predict horizontal and vertical pupil displacements with greater accuracy (in terms of average rms) over short-term time horizons. The potential impact of these improvements on residual wavefront rms is investigated. These results allow to quantify the part of disturbances corrected by retinal imaging systems that are caused by relative displacements of an otherwise fixed or slowy-varying subject-dependent aberration. They also suggest that prediction has a limited impact on wavefront rms and that taking into account PT measurements in real time improves the performance of AO retinal imaging systems.

  9. Eye-pupil displacement and prediction: effects on residual wavefront in adaptive optics retinal imaging

    PubMed Central

    Kulcsár, Caroline; Raynaud, Henri-François; Garcia-Rissmann, Aurea

    2016-01-01

    This paper studies the effect of pupil displacements on the best achievable performance of retinal imaging adaptive optics (AO) systems, using 52 trajectories of horizontal and vertical displacements sampled at 80 Hz by a pupil tracker (PT) device on 13 different subjects. This effect is quantified in the form of minimal root mean square (rms) of the residual phase affecting image formation, as a function of the delay between PT measurement and wavefront correction. It is shown that simple dynamic models identified from data can be used to predict horizontal and vertical pupil displacements with greater accuracy (in terms of average rms) over short-term time horizons. The potential impact of these improvements on residual wavefront rms is investigated. These results allow to quantify the part of disturbances corrected by retinal imaging systems that are caused by relative displacements of an otherwise fixed or slowy-varying subject-dependent aberration. They also suggest that prediction has a limited impact on wavefront rms and that taking into account PT measurements in real time improves the performance of AO retinal imaging systems. PMID:27231607

  10. Adaptive Optics for Direct Detection of Extrasolar Planets: The Gemini Planet Imager

    SciTech Connect

    Macintosh, B; Graham, J; Palmer, D; Doyon, R; Gavel, D; Larkin, J; Oppenheimer, B; Saddlemyer, L; Wallace, J K; Bauman, B; Erikson, D; Poyneer, L; Sivaramakrishnan, A; Soummer, R; Veran, J

    2007-04-24

    The direct detection of photons emitted or reflected by extrasolar planets, spatially resolved from their parent star, is a major frontier in the study of other solar systems. Direct detection will provide statistical information on planets in 5-50 AU orbits, inaccessible to current Doppler searches, and allow spectral characterization of radius, temperature, surface gravity, and perhaps composition. Achieving this will require new dedicated high-contrast instruments. One such system under construction is the Gemini Planet Imager (GPI.) This combines a high-order/high-speed adaptive optics system to control wavefront errors from the Earth's atmosphere, an advanced coronagraph to block diffraction, ultrasmooth optics, a precision infrared interferometer to measure and correct systematic errors, and a integral field spectrograph/polarimeter to image and characterize target planetary systems. We predict that GPI will be able to detect planets with brightness less than 10{sup -7} of their parent star, sufficient to observe warm self-luminous planets around a large population of targets.

  11. Adaptive Optics with a Liquid-Crystal-on-Silicon Spatial Light Modulator and Its Behavior in Retinal Imaging

    NASA Astrophysics Data System (ADS)

    Shirai, Tomohiro; Takeno, Kohei; Arimoto, Hidenobu; Furukawa, Hiromitsu

    2009-07-01

    An adaptive optics system with a brand-new device of a liquid-crystal-on-silicon (LCOS) spatial light modulator (SLM) and its behavior in in vivo imaging of the human retina are described. We confirmed by experiments that closed-loop correction of ocular aberrations of the subject's eye was successfully achieved at the rate of 16.7 Hz in our system to obtain a clear retinal image in real time. The result suggests that an LCOS SLM is one of the promising candidates for a wavefront corrector in a prospective commercial ophthalmic instrument with adaptive optics.

  12. Adaptive optics retinal imaging reveals S-cone dystrophy in tritan color-vision deficiency

    NASA Astrophysics Data System (ADS)

    Baraas, Rigmor C.; Carroll, Joseph; Gunther, Karen L.; Chung, Mina; Williams, David R.; Foster, David H.; Neitz, Maureen

    2007-05-01

    Tritan color-vision deficiency is an autosomal dominant disorder associated with mutations in the short-wavelength-sensitive- (S-) cone-pigment gene. An unexplained feature of the disorder is that individuals with the same mutation manifest different degrees of deficiency. To date, it has not been possible to examine whether any loss of S-cone function is accompanied by physical disruption in the cone mosaic. Two related tritan subjects with the same novel mutation in their S-cone-opsin gene, but different degrees of deficiency, were examined. Adaptive optics was used to obtain high-resolution retinal images, which revealed distinctly different S-cone mosaics consistent with their discrepant phenotypes. In addition, a significant disruption in the regularity of the overall cone mosaic was observed in the subject completely lacking S-cone function. These results taken together with other recent findings from molecular genetics indicate that, with rare exceptions, tritan deficiency is progressive in nature.

  13. A Study of Planetary System Formation and Evolution Using a Dual Channel Adaptive Optics Imaging Polarimeter

    NASA Technical Reports Server (NTRS)

    Potter, Daniel E.

    2003-01-01

    The award I received from the Graduate Student Research Program in 2001, and the renewal award in 2002, supported the gathering and processing of my thesis data, and the presentation of my scientific results at astronomy conferences. Ultimately, this led to a successful defense of my PhD thesis. In the thesis work, I built, calibrated, and observed with the first dual imaging polarimeter ever used in series with a high order adaptive optics system and on an 8 meter class telescope. The instrument was arguably the most sensitive instrument for the high-contrast detection of the scattered light from circumstellar disks. The success of the instrument has provided the impetus for the construction of several similar simultaneous adaptive optics imaging polarimeters. In the course of the observations, a number of circumstellar disks were resolved for the first time in their scattered light, such as the disk around the classical T-tauri stars, LkCa 15 and LkHa 262. A sample of 24 young, nearby, solar-analog stars were observed with the instrument in search of the scattered light signature from debris disks around these stars. Although none of the stars revealed any obvious scattered light signatures, a meaningful limit was placed on the dust population around these stars. Also, a brown dwarf binary system was found to be a companion to one of the solar-analog stars observed, HD130948. A careful monitoring of the orbit of the binary brown dwarf will result in a dynamical mass determination of the objects, providing a valuable calibration of the physical models explaining brown dwarf evolution.

  14. Adaptive optics microscopy enhances image quality in deep layers of CLARITY processed brains of YFP-H mice

    NASA Astrophysics Data System (ADS)

    Reinig, Marc R.; Novack, Samuel W.; Tao, Xiaodong; Ermini, Florian; Bentolila, Laurent A.; Roberts, Dustin G.; MacKenzie-Graham, Allan; Godshalk, S. E.; Raven, M. A.; Kubby, Joel

    2016-03-01

    Optical sectioning of biological tissues has become the method of choice for three-dimensional histological analyses. This is particularly important in the brain were neurons can extend processes over large distances and often whole brain tracing of neuronal processes is desirable. To allow deeper optical penetration, which in fixed tissue is limited by scattering and refractive index mismatching, tissue-clearing procedures such as CLARITY have been developed. CLARITY processed brains have a nearly uniform refractive index and three-dimensional reconstructions at cellular resolution have been published. However, when imaging in deep layers at submicron resolution some limitations caused by residual refractive index mismatching become apparent, as the resulting wavefront aberrations distort the microscopic image. The wavefront can be corrected with adaptive optics. Here, we investigate the wavefront aberrations at different depths in CLARITY processed mouse brains and demonstrate the potential of adaptive optics to enable higher resolution and a better signal-to-noise ratio. Our adaptive optics system achieves high-speed measurement and correction of the wavefront with an open-loop control using a wave front sensor and a deformable mirror. Using adaptive optics enhanced microscopy, we demonstrate improved image quality wavefront, point spread function, and signal to noise in the cortex of YFP-H mice.

  15. In vivo imaging of human retinal microvasculature using adaptive optics scanning light ophthalmoscope fluorescein angiography

    PubMed Central

    Pinhas, Alexander; Dubow, Michael; Shah, Nishit; Chui, Toco Y.; Scoles, Drew; Sulai, Yusufu N.; Weitz, Rishard; Walsh, Joseph B.; Carroll, Joseph; Dubra, Alfredo; Rosen, Richard B.

    2013-01-01

    The adaptive optics scanning light ophthalmoscope (AOSLO) allows visualization of microscopic structures of the human retina in vivo. In this work, we demonstrate its application in combination with oral and intravenous (IV) fluorescein angiography (FA) to the in vivo visualization of the human retinal microvasculature. Ten healthy subjects ages 20 to 38 years were imaged using oral (7 and/or 20 mg/kg) and/or IV (500 mg) fluorescein. In agreement with current literature, there were no adverse effects among the patients receiving oral fluorescein while one patient receiving IV fluorescein experienced some nausea and heaving. We determined that all retinal capillary beds can be imaged using clinically accepted fluorescein dosages and safe light levels according to the ANSI Z136.1-2000 maximum permissible exposure. As expected, the 20 mg/kg oral dose showed higher image intensity for a longer period of time than did the 7 mg/kg oral and the 500 mg IV doses. The increased resolution of AOSLO FA, compared to conventional FA, offers great opportunity for studying physiological and pathological vascular processes. PMID:24009994

  16. A fully automatic framework for cell segmentation on non-confocal adaptive optics images

    NASA Astrophysics Data System (ADS)

    Liu, Jianfei; Dubra, Alfredo; Tam, Johnny

    2016-03-01

    By the time most retinal diseases are diagnosed, macroscopic irreversible cellular loss has already occurred. Earlier detection of subtle structural changes at the single photoreceptor level is now possible, using the adaptive optics scanning light ophthalmoscope (AOSLO). This work aims to develop a fully automatic segmentation framework to extract cell boundaries from non-confocal split-detection AOSLO images of the cone photoreceptor mosaic in the living human eye. Significant challenges include anisotropy, heterogeneous cell regions arising from shading effects, and low contrast between cells and background. To overcome these challenges, we propose the use of: 1) multi-scale Hessian response to detect heterogeneous cell regions, 2) convex hulls to create boundary templates, and 3) circularlyconstrained geodesic active contours to refine cell boundaries. We acquired images from three healthy subjects at eccentric retinal regions and manually contoured cells to generate ground-truth for evaluating segmentation accuracy. Dice coefficient, relative absolute area difference, and average contour distance were 82±2%, 11±6%, and 2.0±0.2 pixels (Mean±SD), respectively. We find that strong shading effects from vessels are a main factor that causes cell oversegmentation and false segmentation of non-cell regions. Our segmentation algorithm can automatically and accurately segment photoreceptor cells on non-confocal AOSLO images, which is the first step in longitudinal tracking of cellular changes in the individual eye over the time course of disease progression.

  17. PENETRATING THE HOMUNCULUS-NEAR-INFRARED ADAPTIVE OPTICS IMAGES OF ETA CARINAE

    SciTech Connect

    Artigau, Etienne; Martin, John C.; Humphreys, Roberta M.; Davidson, Kris; Chesneau, Olivier; Smith, Nathan

    2011-06-15

    Near-infrared adaptive optics imaging with the Near-Infrared Coronagraphic Imager (NICI) and NaCO reveal what appears to be a three-winged or lobed pattern, the 'butterfly nebula', outlined by bright Br{gamma} and H{sub 2} emission and light scattered by dust. In contrast, the [Fe II] emission does not follow the outline of the wings, but shows an extended bipolar distribution which is tracing the Little Homunculus ejected in {eta} Car's second or lesser eruption in the 1890s. Proper motions measured from the combined NICI and NaCO images together with radial velocities show that the knots and filaments that define the bright rims of the butterfly were ejected at two different epochs corresponding approximately to the great eruption and the second eruption. Most of the material is spatially distributed 10{sup 0}-20{sup 0} above and below the equatorial plane apparently behind the Little Homunculus and the larger SE lobe. The equatorial debris either has a wide opening angle or the clumps were ejected at different latitudes relative to the plane. The butterfly is not a coherent physical structure or equatorial torus but spatially separate clumps and filaments ejected at different times, and now 2000-4000 AU from the star.

  18. An adaptive optics imaging system based on a high-resolution liquid crystal on silicon device.

    PubMed

    Mu, Quanquan; Cao, Zhaoliang; Hu, Lifa; Li, Dayu; Xuan, Li

    2006-09-01

    An adaptive optics imaging system is introduced in this paper. A high resolution liquid crystal on silicon (LCOS) device was used as a phase only wave front corrector instead of a conversional deformable mirror. The wave front aberration was detected by a Shack-Hartmann (SH) wave front sensor, which has lambda/100 rms wave front measurement accuracy. Under this construction 0.09lambda (lambda=0.6328microm) Peak to Valley correction precision was reached. Further more, some low frequency hot convection turbulence induced by an electric iron was compensated in real time at the same precision. The Modulation Transfer Function (MTF) of this system was also measured before and after wave front correction. Under the active correction of LCOS, the system reached the diffraction limited resolution approximately 65l p/mm on the horizontal direction. All of this showed the ability of using this device in high resolution, low temporal turbulence imaging system, such as retinal imaging, to improve the resolution performance.

  19. Innovations in adaptive optics imaging and spectroscopy: OSIRIS, galaxy surveys, and galaxy mergers

    NASA Astrophysics Data System (ADS)

    Barczys, Matthew Michael

    This thesis presents the design, construction, and installation of two instruments designed for use with the Keck Telescopes' Adaptive Optics (AO) Systems. It also presents the results of a near-infrared AO survey to measure the pair fraction in faint field galaxies. The instrumentation portion of the thesis concentrates on two main projects, OSIRIS, a near-infrared integral- field spectrograph, and SHARC, a near-infrared camera. OSIRIS is a facility- class instrument at the Keck Observatory, and offers a relatively new spectroscopic capability of simultaneously acquiring infrared spectra ( R ~ 3900) at up to 3000 contiguous locations in an AO-corrected focal plane. SHARC is a camera developed for the Keck AO Team to use with AO-related engineering tasks, including testing and commissioning of the Next-Generation Wavefront Controller. SHARC images a field of up to [Special characters omitted.] on a side, at a spatial resolution of [Special characters omitted.] per pixel throughout the near-infrared (1-2.5 mm). Both instruments were developed in the Infrared Laboratory of the UCLA Department of Physics and Astronomy. The remainder of the thesis describes two near-infrared AO galaxy surveys carried out during the construction of OSIRIS using another instrument partially developed by UCLA. The Keck Observatory Near-Infrared AO Camera (NIRC2) was used to image nearly 700 faint field galaxies (mostly at z ~ 0.5-1.5), with the goal of measuring the near-infrared galaxy pair fraction. This is the first time that AO has been used to measure the pair fraction in field galaxies, and provides a unique opportunity to probe close companions (less than 1 '' ) in the near-infrared. Our measured pair fraction is 0.13 ± 0.04 in both the H - and K ' -bands (1.63mm and 2.12mm) and provides an independent check of optical pair fraction measurements made using the Hubble Space Telescope (HST). While most pairs are observed to have comparable brightness in optical imaging ( dm < 2 mag

  20. Adaptive optimization of reference intensity for optical coherence imaging using galvanometric mirror tilting method

    NASA Astrophysics Data System (ADS)

    Kim, Ji-hyun; Han, Jae-Ho; Jeong, Jichai

    2015-09-01

    Integration time and reference intensity are important factors for achieving high signal-to-noise ratio (SNR) and sensitivity in optical coherence tomography (OCT). In this context, we present an adaptive optimization method of reference intensity for OCT setup. The reference intensity is automatically controlled by tilting a beam position using a Galvanometric scanning mirror system. Before sample scanning, the OCT system acquires two dimensional intensity map with normalized intensity and variables in color spaces using false-color mapping. Then, the system increases or decreases reference intensity following the map data for optimization with a given algorithm. In our experiments, the proposed method successfully corrected the reference intensity with maintaining spectral shape, enabled to change integration time without manual calibration of the reference intensity, and prevented image degradation due to over-saturation and insufficient reference intensity. Also, SNR and sensitivity could be improved by increasing integration time with automatic adjustment of the reference intensity. We believe that our findings can significantly aid in the optimization of SNR and sensitivity for optical coherence tomography systems.

  1. Robotic Laser Adaptive Optics Imaging of 715 Kepler Exoplanet Candidates Using Robo-AO

    NASA Astrophysics Data System (ADS)

    Law, Nicholas M.; Morton, Tim; Baranec, Christoph; Riddle, Reed; Ravichandran, Ganesh; Ziegler, Carl; Johnson, John Asher; Tendulkar, Shriharsh P.; Bui, Khanh; Burse, Mahesh P.; Das, H. K.; Dekany, Richard G.; Kulkarni, Shrinivas; Punnadi, Sujit; Ramaprakash, A. N.

    2014-08-01

    The Robo-AO Kepler Planetary Candidate Survey is observing every Kepler planet candidate host star with laser adaptive optics imaging to search for blended nearby stars, which may be physically associated companions and/or responsible for transit false positives. In this paper, we present the results from the 2012 observing season, searching for stars close to 715 Kepler planet candidate hosts. We find 53 companions, 43 of which are new discoveries. We detail the Robo-AO survey data reduction methods including a method of using the large ensemble of target observations as mutual point-spread-function references, along with a new automated companion-detection algorithm designed for large adaptive optics surveys. Our survey is sensitive to objects from ≈0.''15 to 2.''5 separation, with magnitude differences up to Δm ≈ 6. We measure an overall nearby-star probability for Kepler planet candidates of 7.4% ± 1.0%, and calculate the effects of each detected nearby star on the Kepler-measured planetary radius. We discuss several Kepler Objects of Interest (KOIs) of particular interest, including KOI-191 and KOI-1151, which are both multi-planet systems with detected stellar companions whose unusual planetary system architecture might be best explained if they are "coincident multiple" systems, with several transiting planets shared between the two stars. Finally, we find 98% confidence evidence that short-period giant planets are two to three times more likely than longer-period planets to be found in wide stellar binaries.

  2. Robotic laser adaptive optics imaging of 715 Kepler exoplanet candidates using Robo-AO

    SciTech Connect

    Law, Nicholas M.; Ziegler, Carl; Morton, Tim; Riddle, Reed; Tendulkar, Shriharsh P.; Bui, Khanh; Dekany, Richard G.; Kulkarni, Shrinivas; Punnadi, Sujit; Baranec, Christoph; Ravichandran, Ganesh; Johnson, John Asher; Burse, Mahesh P.; Das, H. K.; Ramaprakash, A. N.

    2014-08-10

    The Robo-AO Kepler Planetary Candidate Survey is observing every Kepler planet candidate host star with laser adaptive optics imaging to search for blended nearby stars, which may be physically associated companions and/or responsible for transit false positives. In this paper, we present the results from the 2012 observing season, searching for stars close to 715 Kepler planet candidate hosts. We find 53 companions, 43 of which are new discoveries. We detail the Robo-AO survey data reduction methods including a method of using the large ensemble of target observations as mutual point-spread-function references, along with a new automated companion-detection algorithm designed for large adaptive optics surveys. Our survey is sensitive to objects from ≈0.''15 to 2.''5 separation, with magnitude differences up to Δm ≈ 6. We measure an overall nearby-star probability for Kepler planet candidates of 7.4% ± 1.0%, and calculate the effects of each detected nearby star on the Kepler-measured planetary radius. We discuss several Kepler Objects of Interest (KOIs) of particular interest, including KOI-191 and KOI-1151, which are both multi-planet systems with detected stellar companions whose unusual planetary system architecture might be best explained if they are 'coincident multiple' systems, with several transiting planets shared between the two stars. Finally, we find 98% confidence evidence that short-period giant planets are two to three times more likely than longer-period planets to be found in wide stellar binaries.

  3. Adaptive Optics for Large Telescopes

    SciTech Connect

    Olivier, S

    2008-06-27

    The use of adaptive optics was originally conceived by astronomers seeking to correct the blurring of images made with large telescopes due to the effects of atmospheric turbulence. The basic idea is to use a device, a wave front corrector, to adjust the phase of light passing through an optical system, based on some measurement of the spatial variation of the phase transverse to the light propagation direction, using a wave front sensor. Although the original concept was intended for application to astronomical imaging, the technique can be more generally applied. For instance, adaptive optics systems have been used for several decades to correct for aberrations in high-power laser systems. At Lawrence Livermore National Laboratory (LLNL), the world's largest laser system, the National Ignition Facility, uses adaptive optics to correct for aberrations in each of the 192 beams, all of which must be precisely focused on a millimeter scale target in order to perform nuclear physics experiments.

  4. Penetrating the Homunculus—Near-Infrared Adaptive Optics Images of Eta Carinae

    NASA Astrophysics Data System (ADS)

    Artigau, Étienne; Martin, John C.; Humphreys, Roberta M.; Davidson, Kris; Chesneau, Olivier; Smith, Nathan

    2011-06-01

    Near-infrared adaptive optics imaging with the Near-Infrared Coronagraphic Imager (NICI) and NaCO reveal what appears to be a three-winged or lobed pattern, the "butterfly nebula," outlined by bright Brγ and H2 emission and light scattered by dust. In contrast, the [Fe II] emission does not follow the outline of the wings, but shows an extended bipolar distribution which is tracing the Little Homunculus ejected in η Car's second or lesser eruption in the 1890s. Proper motions measured from the combined NICI and NaCO images together with radial velocities show that the knots and filaments that define the bright rims of the butterfly were ejected at two different epochs corresponding approximately to the great eruption and the second eruption. Most of the material is spatially distributed 10°-20° above and below the equatorial plane apparently behind the Little Homunculus and the larger SE lobe. The equatorial debris either has a wide opening angle or the clumps were ejected at different latitudes relative to the plane. The butterfly is not a coherent physical structure or equatorial torus but spatially separate clumps and filaments ejected at different times, and now 2000-4000 AU from the star. Based on observations obtained at the Gemini Observatory (program ID: GS-2008B-DD-6), which is operated by the Association of Universities for Research in Astronomy, Inc., under a cooperative agreement with the NSF on behalf of the Gemini partnership: the National Science Foundation (United States), the Science and Technology Facilities Council (United Kingdom), the National Research Council (Canada), CONICYT (Chile), the Australian Research Council (Australia), Ministério da Ciência e Tecnologia (Brazil), and Ministerio de Ciencia, Tecnología e Innovación Productiva (Argentina).

  5. Lucky Imaging Adaptive Optics of the brown dwarf binary GJ569Bab

    NASA Astrophysics Data System (ADS)

    Femenía, B.; Rebolo, R.; Pérez-Prieto, J. A.; Hildebrandt, S. R.; Labadie, L.; Pérez-Garrido, A.; Béjar, V. J. S.; Díaz-Sánchez, A.; Villó, I.; Oscoz, A.; López, R.; Rodríguez, L. F.; Piqueras, J.

    2011-05-01

    The potential of combining Adaptive Optics (AO) and Lucky Imaging (LI) to achieve high-precision astrometry and differential photometry in the optical is investigated by conducting observations of the close 0.1 arcsec brown dwarf binary GJ569Bab. We took 50 000 I-band images with our LI instrument FastCam attached to NAOMI, the 4.2-m William Herschel Telescope (WHT) AO facility. In order to extract the most of the astrometry and photometry of the GJ569Bab system we have resorted to a PSF fitting technique using the primary star GJ569A as a suitable PSF reference which exhibits an I-band magnitude of 7.78 ± 0.03. The AO+LI observations at WHT were able to resolve the binary system GJ569Bab located at 4.92 ± 0.05 arcsec from GJ569A. We measure a separation of 98.4 ± 1.1 mas and I-band magnitudes of 13.86 ± 0.03 and 14.48 ± 0.03 and I-J colours of 2.72 ± 0.08 and 2.83 ± 0.08 for the Ba and Bb components, respectively. Our study rules out the presence of any other companion to GJ569A down to magnitude I˜ 17 at distances larger than 1 arcsec. The I-J colours measured are consistent with M8.5-M9 spectral types for the Ba and Bb components. The available dynamical, photometric and spectroscopic data are consistent with a binary system with Ba being slightly (10-20 per cent) more massive than Bb. We obtain new orbital parameters which are in good agreement with those in the literature. Based on service observations made with the 4.2-m William Herschel Telescope (WHT) operated on the island of La Palma by the Isaac Newton Group and on observations made with the Nordic Optical Telescope (NOT), operated on the island of La Palma jointly by Denmark, Finland, Iceland, Norway and Sweden, in the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofísica de Canarias.

  6. First significant image improvement from a sodium-layer laser guide star adaptive optics system at Lick Observatory

    SciTech Connect

    Olivier, S.S.; Max, C.E.; Friedman, H.W.; An, J.; Avicola, K.; Beeman, B.V.; Bissinger, H.D.; Brase, J.M.; Erbert, G.V.; Gavel, D.T.; Kanz, K.; Macintosh, B.; Neeb, K.P.; Waltjen, K.E.

    1997-07-14

    Atmospheric turbulence severely limits the resolution of ground-based telescopes. Adaptive optics can correct for the aberrations caused by the atmosphere, but requires a bright wavefront reference source in close angular proximity to the object being imaged. Since natural reference stars of the necessary brightness are relatively rare, methods of generating artificial reference beacons have been under active investigation for more than a decade. In this paper, we report the first significant image improvement achieved using a sodium-layer laser guide star as a wavefront reference for a high- order adaptive optics system. An artificial beacon was created by resonant scattering from atomic sodium in the mesosphere, at an altitude of 95 km. Using this laser guide star, an adaptive optics system on the 3 m Shane Telescope at Lick Observatory produced a factor of 2.4 increase in peak intensity and a factor of 2 decrease in full width at half maximum of a stellar image, compared with image motion compensation alone. The Strehl ratio when using the laser guide star as the reference was 65% of that obtained with a natural guide star, and the image full widths at half maximum were identical, 0.3 arc sec, using either the laser or the natural guide star. This sodium-layer laser guide star technique holds great promise for the world`s largest telescopes. 24 refs., 4 figs., 1 tab.

  7. Understanding the changes of cone reflectance in adaptive optics flood illumination retinal images over three years.

    PubMed

    Mariotti, Letizia; Devaney, Nicholas; Lombardo, Giuseppe; Lombardo, Marco

    2016-07-01

    Although there is increasing interest in the investigation of cone reflectance variability, little is understood about its characteristics over long time scales. Cone detection and its automation is now becoming a fundamental step in the assessment and monitoring of the health of the retina and in the understanding of the photoreceptor physiology. In this work we provide an insight into the cone reflectance variability over time scales ranging from minutes to three years on the same eye, and for large areas of the retina (≥ 2.0 × 2.0 degrees) at two different retinal eccentricities using a commercial adaptive optics (AO) flood illumination retinal camera. We observed that the difference in reflectance observed in the cones increases with the time separation between the data acquisitions and this may have a negative impact on algorithms attempting to track cones over time. In addition, we determined that displacements of the light source within 0.35 mm of the pupil center, which is the farthest location from the pupil center used by operators of the AO camera to acquire high-quality images of the cone mosaic in clinical studies, does not significantly affect the cone detection and density estimation.

  8. Retina imaging system with adaptive optics for the eye with or without myopia

    NASA Astrophysics Data System (ADS)

    Li, Chao; Xia, Mingliang; Jiang, Baoguang; Mu, Quanquan; Chen, Shaoyuan; Xuan, Li

    2009-04-01

    An adaptive optics system for the retina imaging is introduced in the paper. It can be applied to the eye with myopia from 0 to 6 diopters without any adjustment of the system. A high-resolution liquid crystal on silicon (LCOS) device is used as the wave-front corrector. The aberration is detected by a Shack-Harmann wave-front sensor (HASO) that has a Root Mean Square (RMS) measurement accuracy of λ/100 ( λ = 0.633 μm). And an equivalent scale model eye is constructed with a short focal length lens (˜18 mm) and a diffuse reflection object (paper screen) as the retina. By changing the distance between the paper screen and the lens, we simulate the eye with larger diopters than 5 and the depth of field. The RMS value both before and after correction is obtained by the wave-front sensor. After correction, the system reaches the diffraction-limited resolution approximately 230 cycles/mm at the object space. It is proved that if the myopia is smaller than 6 diopters and the depth of field is between -40 and +50 mm, the system can correct the aberration very well.

  9. High contrast L' band adaptive optics imaging to detect extrasolar planets

    NASA Astrophysics Data System (ADS)

    Heinze, Ari; Hinz, Phil; Sivanandam, Suresh; Apai, Daniel; Meyer, Michael

    2006-06-01

    We are carrying out a survey to search for giant extrasolar planets around nearby, moderate-age stars in the mid-infrared L' and M bands (3.8 and 4.8 microns, respectively), using the Clio camera with the adaptive optics system on the MMT telescope. To date we have observed 7 stars, of a total 50 planned, including GJ 450 (distance about 8.55pc, age about 1 billion years, no real companions detected), which we use as our example here. We report the methods we use to obtain extremely high contrast imaging in L', and the performance we have obtained. We find that the rotation of a celestial object over time with respect to a telescope tracking it with an altazimuth mount can be a powerful tool for subtracting telescope-related stellar halo artifacts and detecting planets near bright stars. We have carried out a thorough Monte Carlo simulation demonstrating our ability to detect planets as small as 6 Jupiter masses around GJ 450. The division of a science data set into two independent parts, with companions required to be detected on both in order to be recognized as real, played a crucial role in detecting companions in this simulation. We mention also our discovery of a previously unknown faint stellar companion to another of our survey targets, HD 133002. Followup is needed to confirm this as a physical companion, and to determine its physical properties.

  10. Understanding the changes of cone reflectance in adaptive optics flood illumination retinal images over three years.

    PubMed

    Mariotti, Letizia; Devaney, Nicholas; Lombardo, Giuseppe; Lombardo, Marco

    2016-07-01

    Although there is increasing interest in the investigation of cone reflectance variability, little is understood about its characteristics over long time scales. Cone detection and its automation is now becoming a fundamental step in the assessment and monitoring of the health of the retina and in the understanding of the photoreceptor physiology. In this work we provide an insight into the cone reflectance variability over time scales ranging from minutes to three years on the same eye, and for large areas of the retina (≥ 2.0 × 2.0 degrees) at two different retinal eccentricities using a commercial adaptive optics (AO) flood illumination retinal camera. We observed that the difference in reflectance observed in the cones increases with the time separation between the data acquisitions and this may have a negative impact on algorithms attempting to track cones over time. In addition, we determined that displacements of the light source within 0.35 mm of the pupil center, which is the farthest location from the pupil center used by operators of the AO camera to acquire high-quality images of the cone mosaic in clinical studies, does not significantly affect the cone detection and density estimation. PMID:27446708

  11. Understanding the changes of cone reflectance in adaptive optics flood illumination retinal images over three years

    PubMed Central

    Mariotti, Letizia; Devaney, Nicholas; Lombardo, Giuseppe; Lombardo, Marco

    2016-01-01

    Although there is increasing interest in the investigation of cone reflectance variability, little is understood about its characteristics over long time scales. Cone detection and its automation is now becoming a fundamental step in the assessment and monitoring of the health of the retina and in the understanding of the photoreceptor physiology. In this work we provide an insight into the cone reflectance variability over time scales ranging from minutes to three years on the same eye, and for large areas of the retina (≥ 2.0 × 2.0 degrees) at two different retinal eccentricities using a commercial adaptive optics (AO) flood illumination retinal camera. We observed that the difference in reflectance observed in the cones increases with the time separation between the data acquisitions and this may have a negative impact on algorithms attempting to track cones over time. In addition, we determined that displacements of the light source within 0.35 mm of the pupil center, which is the farthest location from the pupil center used by operators of the AO camera to acquire high-quality images of the cone mosaic in clinical studies, does not significantly affect the cone detection and density estimation. PMID:27446708

  12. Adaptive optics for high-contrast imaging of faint substellar companions

    NASA Astrophysics Data System (ADS)

    Morzinski, Katie M.

    Direct imaging of faint objects around bright stars is challenging because the primary star's diffracted light can overwhelm low-mass companions. Nevertheless, advances in adaptive optics (AO) and high-contrast imaging have revealed the first pictures of extrasolar planets. In this dissertation I employ today's high-contrast AO techniques to image brown dwarfs around stars in the nearby Hyades cluster. Furthermore, I prepare for the next generation of high-contrast AO instrumentation, by qualifying MEMS deformable mirrors for wavefront control in the Gemini Planet Imager. In Part I, I present discovery of 3 new brown dwarfs and 36 low-mass stellar companions to 85 stars in the Hyades, imaged with AO at Keck and Lick Observatories. The "locally-optimized combination of images" (LOCI) image-diversity technique filters out the primary star to reveal faint companions. This survey is complete to the hydrogen-burning limit at separations beyond 20 AU. In the complete sample, multiplicity increases as primary star mass decreases. Additionally, the brown dwarfs are at wide >150 AU separations. Finding this preference for low binding-energy systems is an unexpected result, as the Hyades is 625 Myr old and dynamically relaxed. Future work will continue to explore this trend to understand the dynamical and star formation history of the Hyades. The brown dwarfs are near interesting transition regimes for low-mass objects; therefore, characterizing their atmospheres with spectrophotometry will serve as an important benchmark for our understanding of these cool objects. In Part II, I demonstrate micro-electro-mechanical systems (MEMS) deformable mirrors for high-order wavefront control in the Gemini Planet Imager (GPI). MEMS micromirrors have thousands of degrees of freedom and represent a significant cost efficiency over conventional glass deformable mirrors, making them ideal for high-contrast AO. In Chapter 7, I present experimental evidence that MEMS actuators function well

  13. The Dimensions and Pole of Asteroid (21) Lutetia from Adaptive Optics Images

    NASA Astrophysics Data System (ADS)

    Drummond, Jack D.; Conrad, A.; Merline, W.; Carry, B.

    2009-09-01

    In a campaign to study the Rosetta mission target, asteroid (21) Lutetia, we obtained 81 images on December 2, 2008, at 2.12 microns with adaptive optics (AO) on the Keck-II 10 m telescope. From these nearly consecutive images obtained over a quarter of rotation, we have determined the asteroid's triaxial ellipsoid diameters to be 132x101x76 km, with formal uncertainties of 1 km for the equatorial dimensions, and 31 km for the shortest axis. This latter uncertainty occurs because the observations were made at the relatively high sub-Earth latitude of -69 degrees. From these observations we determine that Lutetia's pole lies at 2000.0 coordinates of RA=48, Dec=+9, or Ecliptic coordinates of [49;-8], with a formal uncertainty radius of 3 deg. (The other possible pole is eliminated by considering its lightcurve history.) The rotational pole derived for the lightcurve inversion model (available at http://astro.troja.mff.cuni.cz/ projects/asteroids3D/web.php), is only 5 deg from ours, but comparing our images to the lightcurve inversion model we find that Lutetia is more pointed than the model. Our technique of deriving the dimensions of asteroids from AO images has been calibrated against Pluto and 4 satellites of Saturn with precise diameters, and we find that any systematic errors can be no more than 1-3%. We acknowledge the assistance of other team members Christophe Dumas (ESO), Peter Tamblyn (SwRI), and Clark Chapman (SwRI). We also thank Hal Weaver (JHU/APL) as the lead for our collaboration with the Rosetta mission. We are grateful for telescope time made available to us by S. Kulkarni and M. Busch (Cal Tech) for a portion of our overall Lutetia effort. We also thank our collaborators on Team Keck, the Keck science staff, for making possible some of the Lutetia observations and for their participation. Additional Lutetia observations were acquired at Gemini North under NOAO time allocation.

  14. The Orion fingers: Near-IR adaptive optics imaging of an explosive protostellar outflow

    NASA Astrophysics Data System (ADS)

    Bally, John; Ginsburg, Adam; Silvia, Devin; Youngblood, Allison

    2015-07-01

    Aims: Adaptive optics (AO) images are used to test the hypothesis that the explosive BN/KL outflow from the Orion OMC1 cloud core was powered by the dynamical decay of a non-hierarchical system of massive stars. Methods: Narrow-band H2, [Fe ii], and broad-band Ks obtained with the Gemini South multi-conjugate AO system GeMS and near-IR imager GSAOI are presented. The images reach resolutions of 0.08 to 0.10'', close to the 0.07'' diffraction limit of the 8-m telescope at 2.12 μm. Comparison with previous AO-assisted observations of sub-fields and other ground-based observations enable measurements of proper motions and the investigation of morphological changes in H2 and [Fe ii] features with unprecedented precision. The images are compared with numerical simulations of compact, high-density clumps moving ~103 times their own diameter through a lower density medium at Mach 103. Results: Several sub-arcsecond H2 features and many [Fe ii] "fingertips" on the projected outskirts of the flow show proper motions of ~300 km s-1. High-velocity, sub-arcsecond H2 knots ("bullets") are seen as far as 140'' from their suspected ejection site. If these knots propagated through the dense Orion A cloud, their survival sets a lower bound on their densities of order 107 cm-3, consistent with an origin within a few au of a massive star and accelerated by a final multi-body dynamic encounter that ejected the BN object and radio source I from OMC1 about 500 yr ago. Conclusions: Over 120 high-velocity bow-shocks propagating in nearly all directions from the OMC1 cloud core provide evidence for an explosive origin for the BN/KL outflow triggered by the dynamic decay of a non-hierarchical system of massive stars. Such events may be linked to the origin of runaway, massive stars. The final set of FITS files is only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (ftp://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/579/A130

  15. Aberration correction during real time in vivo imaging of bone marrow with sensorless adaptive optics confocal microscope

    NASA Astrophysics Data System (ADS)

    Wang, Zhibin; Wei, Dan; Wei, Ling; He, Yi; Shi, Guohua; Wei, Xunbin; Zhang, Yudong

    2014-08-01

    We have demonstrated adaptive correction of specimen-induced aberration during in vivo imaging of mouse bone marrow vasculature with confocal fluorescence microscopy. Adaptive optics system was completed with wavefront sensorless correction scheme based on stochastic parallel gradient descent algorithm. Using image sharpness as the optimization metric, aberration correction was performed based upon Zernike polynomial modes. The experimental results revealed the improved signal and resolution leading to a substantially enhanced image contrast with aberration correction. The image quality of vessels at 38- and 75-μm depth increased three times and two times, respectively. The corrections allowed us to detect clearer bone marrow vasculature structures at greater contrast and improve the signal-to-noise ratio.

  16. Intelligent Optical Systems Using Adaptive Optics

    NASA Technical Reports Server (NTRS)

    Clark, Natalie

    2012-01-01

    Until recently, the phrase adaptive optics generally conjured images of large deformable mirrors being integrated into telescopes to compensate for atmospheric turbulence. However, the development of smaller, cheaper devices has sparked interest for other aerospace and commercial applications. Variable focal length lenses, liquid crystal spatial light modulators, tunable filters, phase compensators, polarization compensation, and deformable mirrors are becoming increasingly useful for other imaging applications including guidance navigation and control (GNC), coronagraphs, foveated imaging, situational awareness, autonomous rendezvous and docking, non-mechanical zoom, phase diversity, and enhanced multi-spectral imaging. The active components presented here allow flexibility in the optical design, increasing performance. In addition, the intelligent optical systems presented offer advantages in size and weight and radiation tolerance.

  17. Coherent optical adaptive techniques.

    PubMed

    Bridges, W B; Brunner, P T; Lazzara, S P; Nussmeier, T A; O'Meara, T R; Sanguinet, J A; Brown, W P

    1974-02-01

    The theory of multidither adaptive optical radar phased arrays is briefly reviewed as an introduction to the experimental results obtained with seven-element linear and three-element triangular array systems operating at 0.6328 microm. Atmospheric turbulence compensation and adaptive tracking capabilities are demonstrated.

  18. Laser Guidestar Satellite for Ground-based Adaptive Optics Imaging of Geosynchronous Satellites and Astronomical Targets

    NASA Astrophysics Data System (ADS)

    Marlow, W. A.; Cahoy, K.; Males, J.; Carlton, A.; Yoon, H.

    2015-12-01

    Real-time observation and monitoring of geostationary (GEO) satellites with ground-based imaging systems would be an attractive alternative to fielding high cost, long lead, space-based imagers, but ground-based observations are inherently limited by atmospheric turbulence. Adaptive optics (AO) systems are used to help ground telescopes achieve diffraction-limited seeing. AO systems have historically relied on the use of bright natural guide stars or laser guide stars projected on a layer of the upper atmosphere by ground laser systems. There are several challenges with this approach such as the sidereal motion of GEO objects relative to natural guide stars and limitations of ground-based laser guide stars; they cannot be used to correct tip-tilt, they are not point sources, and have finite angular sizes when detected at the receiver. There is a difference between the wavefront error measured using the guide star compared with the target due to cone effect, which also makes it difficult to use a distributed aperture system with a larger baseline to improve resolution. Inspired by previous concepts proposed by A.H. Greenaway, we present using a space-based laser guide starprojected from a satellite orbiting the Earth. We show that a nanosatellite-based guide star system meets the needs for imaging GEO objects using a low power laser even from 36,000 km altitude. Satellite guide star (SGS) systemswould be well above atmospheric turbulence and could provide a small angular size reference source. CubeSatsoffer inexpensive, frequent access to space at a fraction of the cost of traditional systems, and are now being deployed to geostationary orbits and on interplanetary trajectories. The fundamental CubeSat bus unit of 10 cm cubed can be combined in multiple units and offers a common form factor allowing for easy integration as secondary payloads on traditional launches and rapid testing of new technologies on-orbit. We describe a 6U CubeSat SGS measuring 10 cm x 20 cm x

  19. Telescope Adaptive Optics Code

    2005-07-28

    The Telescope AO Code has general adaptive optics capabilities plus specialized models for three telescopes with either adaptive optics or active optics systems. It has the capability to generate either single-layer or distributed Kolmogorov turbulence phase screens using the FFT. Missing low order spatial frequencies are added using the Karhunen-Loeve expansion. The phase structure curve is extremely dose to the theoreUcal. Secondly, it has the capability to simulate an adaptive optics control systems. The defaultmore » parameters are those of the Keck II adaptive optics system. Thirdly, it has a general wave optics capability to model the science camera halo due to scintillation from atmospheric turbulence and the telescope optics. Although this capability was implemented for the Gemini telescopes, the only default parameter specific to the Gemini telescopes is the primary mirror diameter. Finally, it has a model for the LSST active optics alignment strategy. This last model is highly specific to the LSST« less

  20. Adaptive Optics Images. II. 12 Kepler Objects of Interest and 15 Confirmed Transiting Planets

    NASA Astrophysics Data System (ADS)

    Adams, E. R.; Dupree, A. K.; Kulesa, C.; McCarthy, D.

    2013-07-01

    All transiting planet observations are at risk of contamination from nearby, unresolved stars. Blends dilute the transit signal, causing the planet to appear smaller than it really is, or producing a false positive detection when the target star is blended with an eclipsing binary. High spatial resolution adaptive optics images are an effective way of resolving most blends. Here we present visual companions and detection limits for 12 Kepler planet candidate host stars, of which 4 have companions within 4''. One system (KOI 1537) consists of two similar-magnitude stars separated by 0.''1, while KOI 174 has a companion at 0.''5. In addition, observations were made of 15 transiting planets that were previously discovered by other surveys. The only companion found within 1'' of a known planet is the previously identified companion to WASP-2b. An additional four systems have companions between 1'' and 4'': HAT-P-30b (3.''7, ΔKs = 2.9), HAT-P-32b (2.''9, ΔKs = 3.4), TrES-1b (2.''3, ΔKs = 7.7), and WASP-P-33b (1.''9, ΔKs = 5.5), some of which have not been reported previously. Depending on the spatial resolution of the transit photometry for these systems, these companion stars may require a reassessment of the planetary parameters derived from transit light curves. For all systems observed, we report the limiting magnitudes beyond which additional fainter objects located 0.''1-4'' from the target may still exist. Based on observations obtained at the MMT Observatory, a joint facility of the Smithsonian Institution and the University of Arizona.

  1. White Light Schlieren Optics Using Bacteriorhodopsin as an Adaptive Image Grid

    NASA Technical Reports Server (NTRS)

    Peale, Robert; Ruffin, Boh; Donahue, Jeff; Barrett, Carolyn

    1996-01-01

    A Schlieren apparatus using a bacteriorhodopsin film as an adaptive image grid with white light illumination is demonstrated for the first time. The time dependent spectral properties of the film are characterized. Potential applications include a single-ended Schlieren system for leak detection.

  2. Adaptive optical zoom sensor.

    SciTech Connect

    Sweatt, William C.; Bagwell, Brett E.; Wick, David Victor

    2005-11-01

    In order to optically vary the magnification of an imaging system, continuous mechanical zoom lenses require multiple optical elements and use fine mechanical motion to precisely adjust the separations between individual or groups of lenses. By incorporating active elements into the optical design, we have designed and demonstrated imaging systems that are capable of variable optical magnification with no macroscopic moving parts. Changing the effective focal length and magnification of an imaging system can be accomplished by adeptly positioning two or more active optics in the optical design and appropriately adjusting the optical power of those elements. In this application, the active optics (e.g. liquid crystal spatial light modulators or deformable mirrors) serve as variable focal-length lenses. Unfortunately, the range over which currently available devices can operate (i.e. their dynamic range) is relatively small. Therefore, the key to this concept is to create large changes in the effective focal length of the system with very small changes in the focal lengths of individual elements by leveraging the optical power of conventional optical elements surrounding the active optics. By appropriately designing the optical system, these variable focal-length lenses can provide the flexibility necessary to change the overall system focal length, and therefore magnification, that is normally accomplished with mechanical motion in conventional zoom lenses.

  3. Concept for image-guided vitreo-retinal fs-laser surgery: adaptive optics and optical coherence tomography for laser beam shaping and positioning

    NASA Astrophysics Data System (ADS)

    Matthias, Ben; Brockmann, Dorothee; Hansen, Anja; Horke, Konstanze; Knoop, Gesche; Gewohn, Timo; Zabic, Miroslav; Krüger, Alexander; Ripken, Tammo

    2015-03-01

    Fs-lasers are well established in ophthalmic surgery as high precision tools for corneal flap cutting during laser in situ keratomileusis (LASIK) and increasingly utilized for cutting the crystalline lens, e.g. in assisting cataract surgery. For addressing eye structures beyond the cornea, an intraoperative depth resolved imaging is crucial to the safety and success of the surgical procedure due to interindividual anatomical disparities. Extending the field of application even deeper to the posterior eye segment, individual eye aberrations cannot be neglected anymore and surgery with fs-laser is impaired by focus degradation. Our demonstrated concept for image-guided vitreo-retinal fs-laser surgery combines adaptive optics (AO) for spatial beam shaping and optical coherence tomography (OCT) for focus positioning guidance. The laboratory setup comprises an adaptive optics assisted 800 nm fs-laser system and is extended by a Fourier domain optical coherence tomography system. Phantom structures are targeted, which mimic tractional epiretinal membranes in front of excised porcine retina within an eye model. AO and OCT are set up to share the same scanning and focusing optics. A Hartmann-Shack sensor is employed for aberration measurement and a deformable mirror for aberration correction. By means of adaptive optics the threshold energy for laser induced optical breakdown is lowered and cutting precision is increased. 3D OCT imaging of typical ocular tissue structures is achieved with sufficient resolution and the images can be used for orientation of the fs-laser beam. We present targeted dissection of the phantom structures and its evaluation regarding retinal damage.

  4. A convergent blind deconvolution method for post-adaptive-optics astronomical imaging

    NASA Astrophysics Data System (ADS)

    Prato, M.; La Camera, A.; Bonettini, S.; Bertero, M.

    2013-06-01

    In this paper, we propose a blind deconvolution method which applies to data perturbed by Poisson noise. The objective function is a generalized Kullback-Leibler (KL) divergence, depending on both the unknown object and unknown point spread function (PSF), without the addition of regularization terms; constrained minimization, with suitable convex constraints on both unknowns, is considered. The problem is non-convex and we propose to solve it by means of an inexact alternating minimization method, whose global convergence to stationary points of the objective function has been recently proved in a general setting. The method is iterative and each iteration, also called outer iteration, consists of alternating an update of the object and the PSF by means of a fixed number of iterations, also called inner iterations, of the scaled gradient projection (SGP) method. Therefore, the method is similar to other proposed methods based on the Richardson-Lucy (RL) algorithm, with SGP replacing RL. The use of SGP has two advantages: first, it allows one to prove global convergence of the blind method; secondly, it allows the introduction of different constraints on the object and the PSF. The specific constraint on the PSF, besides non-negativity and normalization, is an upper bound derived from the so-called Strehl ratio (SR), which is the ratio between the peak value of an aberrated versus a perfect wavefront. Therefore, a typical application, but not a unique one, is to the imaging of modern telescopes equipped with adaptive optics systems for the partial correction of the aberrations due to atmospheric turbulence. In the paper, we describe in detail the algorithm and we recall the results leading to its convergence. Moreover, we illustrate its effectiveness by means of numerical experiments whose results indicate that the method, pushed to convergence, is very promising in the reconstruction of non-dense stellar clusters. The case of more complex astronomical targets is

  5. Adaptive Optics Imaging of Healthy and Abnormal Regions of Retinal Nerve Fiber Bundles of Patients With Glaucoma

    PubMed Central

    Chen, Monica F.; Chui, Toco Y. P.; Alhadeff, Paula; Rosen, Richard B.; Ritch, Robert; Dubra, Alfredo; Hood, Donald C.

    2015-01-01

    Purpose. To better understand the nature of glaucomatous damage of the macula, especially the structural changes seen between relatively healthy and clearly abnormal (AB) retinal regions, using an adaptive optics scanning light ophthalmoscope (AO-SLO). Methods. Adaptive optics SLO images and optical coherence tomography (OCT) vertical line scans were obtained on one eye of seven glaucoma patients, with relatively deep local arcuate defects on the 10-2 visual field test in one (six eyes) or both hemifields (one eye). Based on the OCT images, the retinal nerve fiber (RNF) layer was divided into two regions: (1) within normal limits (WNL), relative RNF layer thickness within mean control values ±2 SD; and (2) AB, relative thickness less than −2 SD value. Results. As seen on AO-SLO, the pattern of AB RNF bundles near the border of the WNL and AB regions differed across eyes. There were normal-appearing bundles in the WNL region of all eyes and AB-appearing bundles near the border with the AB region. This region with AB bundles ranged in extent from a few bundles to the entire AB region in the case of one eye. All other eyes had a large AB region without bundles. However, in two of these eyes, a few bundles were seen within this region of otherwise missing bundles. Conclusions. The AO-SLO images revealed details of glaucomatous damage that are difficult, if not impossible, to see with current OCT technology. Adaptive optics SLO may prove useful in following progression in clinical trials, or in disease management, if AO-SLO becomes widely available and easy to use. PMID:25574048

  6. The Subaru Coronagraphic Extreme Adaptive Optics System: Enabling High-Contrast Imaging on Solar-System Scales

    NASA Astrophysics Data System (ADS)

    Jovanovic, N.; Martinache, F.; Guyon, O.; Clergeon, C.; Singh, G.; Kudo, T.; Garrel, V.; Newman, K.; Doughty, D.; Lozi, J.; Males, J.; Minowa, Y.; Hayano, Y.; Takato, N.; Morino, J.; Kuhn, J.; Serabyn, E.; Norris, B.; Tuthill, P.; Schworer, G.; Stewart, P.; Close, L.; Huby, E.; Perrin, G.; Lacour, S.; Gauchet, L.; Vievard, S.; Murakami, N.; Oshiyama, F.; Baba, N.; Matsuo, T.; Nishikawa, J.; Tamura, M.; Lai, O.; Marchis, F.; Duchene, G.; Kotani, T.; Woillez, J.

    2015-09-01

    The Subaru Coronagraphic Extreme Adaptive Optics (SCExAO) instrument is a multipurpose high-contrast imaging platform designed for the discovery and detailed characterization of exoplanetary systems and serves as a testbed for high-contrast imaging technologies for ELTs. It is a multiband instrument which makes use of light from 600 to 2500 nm, allowing for coronagraphic direct exoplanet imaging of the inner 3λ/D from the stellar host. Wavefront sensing and control are key to the operation of SCExAO. A partial correction of low-order modes is provided by Subaru's facility adaptive optics system with the final correction, including high-order modes, implemented downstream by a combination of a visible pyramid wavefront sensor and a 2000-element deformable mirror. The well-corrected NIR (y-K bands) wavefronts can then be injected into any of the available coronagraphs, including but not limited to the phase-induced amplitude apodization and the vector vortex coronagraphs, both of which offer an inner working angle as low as 1λ/D. Noncommon path, low-order aberrations are sensed with a coronagraphic low-order wavefront sensor in the infrared (IR). Low noise, high frame rate NIR detectors allow for active speckle nulling and coherent differential imaging, while the HAWAII 2RG detector in the HiCIAO imager and/or the CHARIS integral field spectrograph (from mid-2016) can take deeper exposures and/or perform angular, spectral, and polarimetric differential imaging. Science in the visible is provided by two interferometric modules: VAMPIRES and FIRST, which enable subdiffraction limited imaging in the visible region with polarimetric and spectroscopic capabilities respectively. We describe the instrument in detail and present preliminary results both on-sky and in the laboratory.

  7. AIDA: Adaptive Image Deconvolution Algorithm

    NASA Astrophysics Data System (ADS)

    Hom, Erik; Haase, Sebastian; Marchis, Franck

    2013-10-01

    AIDA is an implementation and extension of the MISTRAL myopic deconvolution method developed by Mugnier et al. (2004) (see J. Opt. Soc. Am. A 21:1841-1854). The MISTRAL approach has been shown to yield object reconstructions with excellent edge preservation and photometric precision when used to process astronomical images. AIDA improves upon the original MISTRAL implementation. AIDA, written in Python, can deconvolve multiple frame data and three-dimensional image stacks encountered in adaptive optics and light microscopic imaging.

  8. Advanced Adaptive Optics Technology Development

    SciTech Connect

    Olivier, S

    2001-09-18

    The NSF Center for Adaptive Optics (CfAO) is supporting research on advanced adaptive optics technologies. CfAO research activities include development and characterization of micro-electro-mechanical systems (MEMS) deformable mirror (DM) technology, as well as development and characterization of high-resolution adaptive optics systems using liquid crystal (LC) spatial light modulator (SLM) technology. This paper presents an overview of the CfAO advanced adaptive optics technology development activities including current status and future plans.

  9. Real-time atmospheric imaging and processing with hybrid adaptive optics and hardware accelerated lucky-region fusion (LRF) algorithm

    NASA Astrophysics Data System (ADS)

    Liu, Jony Jiang; Carhart, Gary W.; Beresnev, Leonid A.; Aubailly, Mathieu; Jackson, Christopher R.; Ejzak, Garrett; Kiamilev, Fouad E.

    2014-09-01

    Atmospheric turbulences can significantly deteriorate the performance of long-range conventional imaging systems and create difficulties for target identification and recognition. Our in-house developed adaptive optics (AO) system, which contains high-performance deformable mirrors (DMs) and the fast stochastic parallel gradient decent (SPGD) control mechanism, allows effective compensation of such turbulence-induced wavefront aberrations and result in significant improvement on the image quality. In addition, we developed advanced digital synthetic imaging and processing technique, "lucky-region" fusion (LRF), to mitigate the image degradation over large field-of-view (FOV). The LRF algorithm extracts sharp regions from each image obtained from a series of short exposure frames and fuses them into a final improved image. We further implemented such algorithm into a VIRTEX-7 field programmable gate array (FPGA) and achieved real-time video processing. Experiments were performed by combining both AO and hardware implemented LRF processing technique over a near-horizontal 2.3km atmospheric propagation path. Our approach can also generate a universal real-time imaging and processing system with a general camera link input, a user controller interface, and a DVI video output.

  10. Data reduction pipeline for OSIRIS, the new NIR diffraction-limited imaging field spectrograph for the Keck adaptive optics system

    NASA Astrophysics Data System (ADS)

    Krabbe, Alfred; Gasaway, Tom; Song, Inseok; Iserlohe, Christof; Weiss, Jason; Larkin, James E.; Barczys, Matthew; Lafreniere, David

    2004-09-01

    OSIRIS is a near infrared diffraction limited imaging field spectrograph under development for the Keck observatory adaptive optics system and scheduled for commissioning in fall 2004. Based upon lenslet pupil imaging, diffraction grating, and a 2Kx2K Hawaii2 HgCdTe array, OSIRIS is a highly efficient instrument at the forefront of today's technology. OSIRIS will deliver per readout up to 4096 diffraction limited spectra in a complex interleaved format, requiring new challenges to be met regarding user interaction and data reduction. A data reduction software package is under development, aiming to provide the observer with a facility instrument allowing him to concentrate on science rather than dealing with instrumental as well as telescope and atmosphere related effects. Together with OSIRIS, a pipeline for basic data reduction will be provided for a new Keck instrument for the first time. A status report is presented here together with some aspects of the data reduction pipeline.

  11. SHARP - III. First use of adaptive-optics imaging to constrain cosmology with gravitational lens time delays

    NASA Astrophysics Data System (ADS)

    Chen, Geoff C.-F.; Suyu, Sherry H.; Wong, Kenneth C.; Fassnacht, Christopher D.; Chiueh, Tzihong; Halkola, Aleksi; Hu, I. Shing; Auger, Matthew W.; Koopmans, Léon V. E.; Lagattuta, David J.; McKean, John P.; Vegetti, Simona

    2016-11-01

    Accurate and precise measurements of the Hubble constant are critical for testing our current standard cosmological model and revealing possibly new physics. With Hubble Space Telescope (HST) imaging, each strong gravitational lens system with measured time delays can allow one to determine the Hubble constant with an uncertainty of ˜7 per cent. Since HST will not last forever, we explore adaptive-optics (AO) imaging as an alternative that can provide higher angular resolution than HST imaging but has a less stable point spread function (PSF) due to atmospheric distortion. To make AO imaging useful for time-delay-lens cosmography, we develop a method to extract the unknown PSF directly from the imaging of strongly lensed quasars. In a blind test with two mock data sets created with different PSFs, we are able to recover the important cosmological parameters (time-delay distance, external shear, lens-mass profile slope, and total Einstein radius). Our analysis of the Keck AO image of the strong lens system RXJ 1131-1231 shows that the important parameters for cosmography agree with those based on HST imaging and modelling within 1σ uncertainties. Most importantly, the constraint on the model time-delay distance by using AO imaging with 0.09 arcsec resolution is tighter by ˜50 per cent than the constraint of time-delay distance by using HST imaging with 0.09 arcsec when a power-law mass distribution for the lens system is adopted. Our PSF reconstruction technique is generic and applicable to data sets that have multiple nearby point sources, enabling scientific studies that require high-precision models of the PSF.

  12. NEAR-INFRARED ADAPTIVE OPTICS IMAGING OF INFRARED LUMINOUS GALAXIES: THE BRIGHTEST CLUSTER MAGNITUDE-STAR FORMATION RATE RELATION

    SciTech Connect

    Randriamanakoto, Z.; Väisänen, P.; Escala, A.; Kankare, E.; Kotilainen, J.; Mattila, S.; Ryder, S.

    2013-10-01

    We have established a relation between the brightest super star cluster (SSC) magnitude in a galaxy and the host star formation rate (SFR) for the first time in the near-infrared (NIR). The data come from a statistical sample of ∼40 luminous IR galaxies (LIRGs) and starbursts utilizing K-band adaptive optics imaging. While expanding the observed relation to longer wavelengths, less affected by extinction effects, it also pushes to higher SFRs. The relation we find, M{sub K} ∼ –2.6log SFR, is similar to that derived previously in the optical and at lower SFRs. It does not, however, fit the optical relation with a single optical to NIR color conversion, suggesting systematic extinction and/or age effects. While the relation is broadly consistent with a size-of-sample explanation, we argue physical reasons for the relation are likely as well. In particular, the scatter in the relation is smaller than expected from pure random sampling strongly suggesting physical constraints. We also derive a quantifiable relation tying together cluster-internal effects and host SFR properties to possibly explain the observed brightest SSC magnitude versus SFR dependency.

  13. Analytical expression of long-exposure adaptive-optics-corrected coronagraphic image. First application to exoplanet detection.

    PubMed

    Sauvage, J-F; Mugnier, L M; Rousset, G; Fusco, T

    2010-11-01

    In this paper we derive an analytical model of a long-exposure star image for an adaptive-optics(AO)-corrected coronagraphic imaging system. This expression accounts for static aberrations upstream and downstream of the coronagraphic mask as well as turbulence residuals. It is based on the perfect coronagraph model. The analytical model is validated by means of simulations using the design and parameters of the SPHERE instrument. The analytical model is also compared to a simulated four-quadrant phase-mask coronagraph. Then, its sensitivity to a miscalibration of structure function and upstream static aberrations is studied, and the impact on exoplanet detectability is quantified. Last, a first inversion method is presented for a simulation case using a single monochromatic image with no reference. The obtained result shows a planet detectability increase by two orders of magnitude with respect to the raw image. This analytical model presents numerous potential applications in coronographic imaging, such as exoplanet direct detection, and circumstellar disk observation.

  14. Analytical expression of long-exposure adaptive-optics-corrected coronagraphic image. First application to exoplanet detection.

    PubMed

    Sauvage, J-F; Mugnier, L M; Rousset, G; Fusco, T

    2010-11-01

    In this paper we derive an analytical model of a long-exposure star image for an adaptive-optics(AO)-corrected coronagraphic imaging system. This expression accounts for static aberrations upstream and downstream of the coronagraphic mask as well as turbulence residuals. It is based on the perfect coronagraph model. The analytical model is validated by means of simulations using the design and parameters of the SPHERE instrument. The analytical model is also compared to a simulated four-quadrant phase-mask coronagraph. Then, its sensitivity to a miscalibration of structure function and upstream static aberrations is studied, and the impact on exoplanet detectability is quantified. Last, a first inversion method is presented for a simulation case using a single monochromatic image with no reference. The obtained result shows a planet detectability increase by two orders of magnitude with respect to the raw image. This analytical model presents numerous potential applications in coronographic imaging, such as exoplanet direct detection, and circumstellar disk observation. PMID:21045877

  15. Adaptive Optical Scanning Holography

    PubMed Central

    Tsang, P. W. M.; Poon, Ting-Chung; Liu, J.-P.

    2016-01-01

    Optical Scanning Holography (OSH) is a powerful technique that employs a single-pixel sensor and a row-by-row scanning mechanism to capture the hologram of a wide-view, three-dimensional object. However, the time required to acquire a hologram with OSH is rather lengthy. In this paper, we propose an enhanced framework, which is referred to as Adaptive OSH (AOSH), to shorten the holographic recording process. We have demonstrated that the AOSH method is capable of decreasing the acquisition time by up to an order of magnitude, while preserving the content of the hologram favorably. PMID:26916866

  16. Sodium guide star adaptive optics system for astronomical imaging in the visible and near-infrared

    SciTech Connect

    Gavel, D.T.; Morris, J.R. ); Vernon, R.G. )

    1992-08-01

    We are building an adaptive-optic telescope system that is based on the use of an artificial guide star created by laser-induced fluorescence of the sodium mesospheric layer. This paper discusses the system design for mid-visible to near-infrared compensation of a one meter telescope at Livermore and near-infrared compensation of the ten meter Keck telescope at Mauna Kea. We calculate the expected Strehl ratio and resolution for a 69 channel deformable mirror system and also for a possible 24 channel system upgrade. With the 69 actuator system we expect near diffraction limited resolution, about 0.2 arcsec, with a Strehl ratio of about 0.5 at {gamma}=0.8 {mu}m on the 1m telescope, and resolution of about 0.05 arcsec with a Strehl ratio of about 0.5 at {gamma}=2.0 {mu}m on the 10m telescope. Resolution will be limited by the performance of the tip/tilt correction loop, which uses an off-axis natural guide star as a reference. At Livermore, our design uses an existing high power (1 kW) laser source, which is expected to provide an approximately 6th magnitude artificial guide star. This strong beacon signal allows a short integration time in the wavefront sensor so that temporal changes in the atmospheric turbulence can be tracked accurately. For Mauna Kea, we explore how the system to be built for the Livermore site would perform in the infrared, assuming a 100 W laser source.

  17. Sodium guide star adaptive optics system for astronomical imaging in the visible and near-infrared

    SciTech Connect

    Gavel, D.T.; Morris, J.R. ); Vernon, R.G. )

    1992-03-01

    We are building an adaptive-optic telescope system that is based on the use of an artificial guide star created by laser-induced fluorescence of the sodium mesospheric layer. This paper discusses the system design for mid-visible to near-infrared compensation of a one meter telescope at Livermore and near-infrared compensation of the ten meter Keck telescope at Mauna Kea. We calculate the expected Strehl ratio and resolution for a 69 channel deformable mirror system and also for a possible 241 channel system upgrade. With the 69 actuator system we expect near diffraction limited resolution, about 0.2 arcsec, with a Strehl ratio of about 0.5 at [lambda]=0.8,[mu]m on the 1m telescope, and resolution of about 0.05 arcsec with a Strehl ratio of about 0.5 at [lambda]=2.0 [mu]m on the 10m telescope. Resolution will be limited by the performance of the tip/tilt correction loop, which uses an off-axis natural guide star as a reference. The effects which degrade tip/tilt correction are described in a companion paper. At Livermore, our design uses an existing high power (1 kW) laser source, which is expected to provide an approximately 6'th magnitude artificial guide star. This strong beacon signal allows a short integration time in the wavefront sensor so that temporal changes in the atmospheric turbulence can be tracked accurately. For Mauna Kea, we explore how the system to be built for the Livermore site would perform in the infrared, assuming a 100 W laser source.

  18. Sodium guide star adaptive optics system for astronomical imaging in the visible and near-infrared

    SciTech Connect

    Gavel, D.T.; Morris, J.R.; Vernon, R.G.

    1992-03-01

    We are building an adaptive-optic telescope system that is based on the use of an artificial guide star created by laser-induced fluorescence of the sodium mesospheric layer. This paper discusses the system design for mid-visible to near-infrared compensation of a one meter telescope at Livermore and near-infrared compensation of the ten meter Keck telescope at Mauna Kea. We calculate the expected Strehl ratio and resolution for a 69 channel deformable mirror system and also for a possible 241 channel system upgrade. With the 69 actuator system we expect near diffraction limited resolution, about 0.2 arcsec, with a Strehl ratio of about 0.5 at {lambda}=0.8,{mu}m on the 1m telescope, and resolution of about 0.05 arcsec with a Strehl ratio of about 0.5 at {lambda}=2.0 {mu}m on the 10m telescope. Resolution will be limited by the performance of the tip/tilt correction loop, which uses an off-axis natural guide star as a reference. The effects which degrade tip/tilt correction are described in a companion paper. At Livermore, our design uses an existing high power (1 kW) laser source, which is expected to provide an approximately 6`th magnitude artificial guide star. This strong beacon signal allows a short integration time in the wavefront sensor so that temporal changes in the atmospheric turbulence can be tracked accurately. For Mauna Kea, we explore how the system to be built for the Livermore site would perform in the infrared, assuming a 100 W laser source.

  19. Passive adaptive imaging through turbulence

    NASA Astrophysics Data System (ADS)

    Tofsted, David

    2016-05-01

    Standard methods for improved imaging system performance under degrading optical turbulence conditions typically involve active adaptive techniques or post-capture image processing. Here, passive adaptive methods are considered where active sources are disallowed, a priori. Theoretical analyses of short-exposure turbulence impacts indicate that varying aperture sizes experience different degrees of turbulence impacts. Smaller apertures often outperform larger aperture systems as turbulence strength increases. This suggests a controllable aperture system is advantageous. In addition, sub-aperture sampling of a set of training images permits the system to sense tilts in different sub-aperture regions through image acquisition and image cross-correlation calculations. A four sub-aperture pattern supports corrections involving five realizable operating modes (beyond tip and tilt) for removing aberrations over an annular pattern. Progress to date will be discussed regarding development and field trials of a prototype system.

  20. ADAPTIVE OPTICS IMAGING OF VY CANIS MAJORIS AT 2-5 μm WITH LBT/LMIRCam

    SciTech Connect

    Shenoy, Dinesh P.; Jones, Terry J.; Humphreys, Roberta M.; Marengo, Massimo; Leisenring, Jarron M.; Nelson, Matthew J.; Wilson, John C.; Skrutskie, Michael F.; Hinz, Philip M.; Hoffmann, William F.; Bailey, Vanessa; Skemer, Andrew; Rodigas, Timothy; Vaitheeswaran, Vidhya

    2013-10-01

    We present adaptive optics images of the extreme red supergiant VY Canis Majoris in the K{sub s} , L', and M bands (2.15-4.8 μm) made with LMIRCam on the Large Binocular Telescope. The peculiar ''Southwest Clump'' previously imaged from 1 to 2.2 μm appears prominently in all three filters. We find its brightness is due almost entirely to scattering, with the contribution of thermal emission limited to at most 25%. We model its brightness as optically thick scattering from silicate dust grains using typical size distributions. We find a lower limit mass for this single feature of 5 × 10{sup –3} M {sub ☉} to 2.5 × 10{sup –2} M {sub ☉} depending on the assumed gas-to-dust ratio. The presence of the Clump as a distinct feature with no apparent counterpart on the other side of the star is suggestive of an ejection event from a localized region of the star and is consistent with VY CMa's history of asymmetric high-mass-loss events.

  1. Optimization of adaptive-optics systems closed-loop bandwidth settings to maximize imaging-system performance.

    PubMed

    Brigantic, R T; Roggemann, M C; Welsh, B M; Bauer, K W

    1998-02-10

    We present the results of research aimed at optimizing adaptive-optics closed-loop bandwidth settings to maximize imaging-system performance. The optimum closed-loop bandwidth settings are determined as a function of target-object light levels and atmospheric seeing conditions. Our work shows that, for bright objects, the optimum closed-loop bandwidth is near the Greenwood frequency. However, for dim objects without the use of a laser beacon the preferred closed-loop bandwidth settings are a small fraction of the Greenwood frequency. In addition, under low light levels selection of the proper closed-loop bandwidth is more critical for achieving maximum performance than it is under high light levels. We also present a strategy for selecting the closed-loop bandwidth to provide robust system performance for different target-object light levels.

  2. Physics and Advanced Technologies LDRD Final Report:Adaptive Optics Imaging and Spectroscopy of the Solar System

    SciTech Connect

    Gibbard, S; Max, C; Macintosh, B; Grossman, A

    2004-01-21

    This focus of this project was the investigation of the planets Uranus and Neptune and Saturn's moon Titan using adaptive optics imaging and spectroscopy at the 10-meter W.M. Keck Telescopes. These bodies share a common type of atmosphere, one that is rich in methane and has a hydrocarbon haze layer produced by methane photolysis. Neptune and Uranus have atmospheric features which change on short timescales; we have investigated their altitude, composition, and connection to events occurring deeper in the planets' tropospheres. Titan has a solid surface located under its atmosphere, the composition of which is still quite uncertain. With spectra that sample the vertical structure of the atmosphere and narrowband observations that selectively probe Titan's surface we have determined the surface reflectivity of Titan at near-infrared wavelengths.

  3. The ERIS adaptive optics system

    NASA Astrophysics Data System (ADS)

    Marchetti, Enrico; Fedrigo, Enrico; Le Louarn, Miska; Madec, Pierre-Yves; Soenke, Christian; Brast, Roland; Conzelmann, Ralf; Delabre, Bernard; Duchateau, Michel; Frank, Christoph; Klein, Barbara; Amico, Paola; Hubin, Norbert; Esposito, Simone; Antichi, Jacopo; Carbonaro, Luca; Puglisi, Alfio; Quirós-Pacheco, Fernando; Riccardi, Armando; Xompero, Marco

    2014-07-01

    The Enhanced Resolution Imager and Spectrograph (ERIS) is the new Adaptive Optics based instrument for ESO's VLT aiming at replacing NACO and SINFONI to form a single compact facility with AO fed imaging and integral field unit spectroscopic scientific channels. ERIS completes the instrument suite at the VLT adaptive telescope. In particular it is equipped with a versatile AO system that delivers up to 95% Strehl correction in K band for science observations up to 5 micron It comprises high order NGS and LGS correction enabling the observation from exoplanets to distant galaxies with a large sky coverage thanks to the coupling of the LGS WFS with the high sensitivity of its visible WFS and the capability to observe in dust embedded environment thanks to its IR low order WFS. ERIS will be installed at the Cassegrain focus of the VLT unit hosting the Adaptive Optics Facility (AOF). The wavefront correction is provided by the AOF deformable secondary mirror while the Laser Guide Star is provided by one of the four launch units of the 4 Laser Guide Star Facility for the AOF. The overall layout of the ERIS AO system is extremely compact and highly optimized: the SPIFFI spectrograph is fed directly by the Cassegrain focus and both the NIX's (IR imager) and SPIFFI's entrance windows work as visible/infrared dichroics. In this paper we describe the concept of the ERIS AO system in detail, starting from the requirements and going through the estimated performance, the opto-mechanical design and the Real-Time Computer design.

  4. Novel Adaptive Optics concepts : wavefront sensing with sodium laser guide stars at Extemely Large Telescopes and simultaneous differential imaging

    NASA Astrophysics Data System (ADS)

    Kellner, Stephan Albert

    2005-12-01

    Since more than 15 years, Adaptive Optics (AO) is a proven concept to reach diffraction limited imaging at modern astronomical telescopes. In the case of next generation telescopes (Extremely Large Telescopes (ELTs)) with aperture diameters of up to 100m, sodium laser guide star based multi-conjugated-AO systems will be a basic requirement to exploit their full capability in terms of resolution and light concentration. A drawback of such an approach emerges in the finite distance and vertical extent of the sodium beacon in the mesosphere with respect to the telescope. This induces effects such as perspective elongation, where conventional wavefront sensing mechanisms will fail. Although several engineering concepts are under development to counteract these constraints at the cost of overall light efficiency and increased system complexity, this thesis proposes a novel kind of wavefront sensing technique to overcome the imposed limitations in a more natural way. The sensing technique is composed of two independently working sensors, a reflective rod and a mask with circular slits, each a representative of a novel wavefront sensor class, the so called z-invariant and Inverse Bessel Beam technique. Both are discussed in this thesis with a focus on the Inverse Bessel Beam technique. The latter is compared to alternative concepts such as temporal gating, with respect to the photon efficiency. Furthermore, the reflective rod was tested for its feasibility in laboratory conditions and in a more realistic environment at the William Herschel Telescope (WHT) at La Palma. With this test run its sensing principle has been verified. A novel technique already intensively used at 8m class telescopes is Simultaneous Differential Imaging. The direct detection of giant extra-solar planets is and will be a major science driver for galactic astronomy in the coming years. Modern telescope facilities such as the VLT reach, by means of adaptive optics, potentially the capability in terms

  5. Keck adaptive optics: control subsystem

    SciTech Connect

    Brase, J.M.; An, J.; Avicola, K.

    1996-03-08

    Adaptive optics on the Keck 10 meter telescope will provide an unprecedented level of capability in high resolution ground based astronomical imaging. The system is designed to provide near diffraction limited imaging performance with Strehl {gt} 0.3 n median Keck seeing of r0 = 25 cm, T =10 msec at 500 nm wavelength. The system will be equipped with a 20 watt sodium laser guide star to provide nearly full sky coverage. The wavefront control subsystem is responsible for wavefront sensing and the control of the tip-tilt and deformable mirrors which actively correct atmospheric turbulence. The spatial sampling interval for the wavefront sensor and deformable mirror is de=0.56 m which gives us 349 actuators and 244 subapertures. This paper summarizes the wavefront control system and discusses particular issues in designing a wavefront controller for the Keck telescope.

  6. High-Resolution Imaging by Adaptive Optics Scanning Laser Ophthalmoscopy Reveals Two Morphologically Distinct Types of Retinal Hard Exudates.

    PubMed

    Yamaguchi, Muneo; Nakao, Shintaro; Kaizu, Yoshihiro; Kobayashi, Yoshiyuki; Nakama, Takahito; Arima, Mitsuru; Yoshida, Shigeo; Oshima, Yuji; Takeda, Atsunobu; Ikeda, Yasuhiro; Mukai, Shizuo; Ishibashi, Tatsuro; Sonoda, Koh-Hei

    2016-01-01

    Histological studies from autopsy specimens have characterized hard exudates as a composition of lipid-laden macrophages or noncellular materials including lipid and proteinaceous substances (hyaline substances). However, the characteristics of hard exudates in living patients have not been examined due to insufficient resolution of existing equipment. In this study, we used adaptive optics scanning laser ophthalmoscopy (AO-SLO) to examine the characteristics of hard exudates in patients with retinal vascular diseases. High resolution imaging using AO-SLO enables morphological classification of retinal hard exudates into two types, which could not be distinguished either on fundus examination or by spectral domain optical coherence tomography (SD-OCT). One, termed a round type, consisted of an accumulation of spherical particles (average diameter of particles: 26.9 ± 4.4 μm). The other, termed an irregular type, comprised an irregularly shaped hyper-reflective deposition. The retinal thickness in regions with round hard exudates was significantly greater than the thickness in regions with irregular hard exudates (P = 0.01 →0.02). This differentiation of retinal hard exudates in patients by AO-SLO may help in understanding the pathogenesis and clinical prognosis of retinal vascular diseases. PMID:27641223

  7. High-Resolution Imaging by Adaptive Optics Scanning Laser Ophthalmoscopy Reveals Two Morphologically Distinct Types of Retinal Hard Exudates

    PubMed Central

    Yamaguchi, Muneo; Nakao, Shintaro; Kaizu, Yoshihiro; Kobayashi, Yoshiyuki; Nakama, Takahito; Arima, Mitsuru; Yoshida, Shigeo; Oshima, Yuji; Takeda, Atsunobu; Ikeda, Yasuhiro; Mukai, Shizuo; Ishibashi, Tatsuro; Sonoda, Koh-hei

    2016-01-01

    Histological studies from autopsy specimens have characterized hard exudates as a composition of lipid-laden macrophages or noncellular materials including lipid and proteinaceous substances (hyaline substances). However, the characteristics of hard exudates in living patients have not been examined due to insufficient resolution of existing equipment. In this study, we used adaptive optics scanning laser ophthalmoscopy (AO-SLO) to examine the characteristics of hard exudates in patients with retinal vascular diseases. High resolution imaging using AO-SLO enables morphological classification of retinal hard exudates into two types, which could not be distinguished either on fundus examination or by spectral domain optical coherence tomography (SD-OCT). One, termed a round type, consisted of an accumulation of spherical particles (average diameter of particles: 26.9 ± 4.4 μm). The other, termed an irregular type, comprised an irregularly shaped hyper-reflective deposition. The retinal thickness in regions with round hard exudates was significantly greater than the thickness in regions with irregular hard exudates (P = 0.01 →0.02). This differentiation of retinal hard exudates in patients by AO-SLO may help in understanding the pathogenesis and clinical prognosis of retinal vascular diseases. PMID:27641223

  8. Data Reduction Pipeline for OSIRIS, the new NIR Diffraction Limited Imaging Field Spectrometer for the Keck Adaptive Optics System

    NASA Astrophysics Data System (ADS)

    Krabbe, Alfred; Gasaway, Thomas M.; Weiss, Jason; Larkin, James E.; Barczys, Matthew; Quirrenbach, Andreas; LaFreniere, David

    2002-12-01

    OSIRIS is a near infrared diffraction limited imaging field spectrometer under development for the Keck observatory adaptive optics system. Based upon lenslet pupil imaging, diffraction grating, and a 2K×2K Hawaii2 HgCdTe array, OSIRIS is a highly efficient instrument at the forefront of today"s technology. OSIRIS will deliver per readout up to 4096 diffraction limited spectra in a complex interleaved format, requiring new challenges to be met regarding user interaction and data reduction. A data reduction software package is under development, aiming to provide the observer with a facility instrument allowing him to concentrate on science rather than dealing with instrumental as well as telescope and atmosphere related effects. Together with OSIRIS, a pipeline for basic data reduction will be provided for a new Keck instrument for the first time. Some aspects of the data reduction pipeline will be presented here. The OSIRIS instrument as such, the astronomical background as well as other software tools were presented elsewhere on this conference.

  9. Adaptive Optics Imaging of the CLASS Gravitational Lens System B1359+154 with the Canada-France-Hawaii Telescope.

    PubMed

    Rusin; Hall; Nichol; Marlow; Richards; Myers

    2000-04-20

    We present adaptive optics imaging of the CLASS gravitational lens system B1359+154 obtained with the Canada-France-Hawaii Telescope (CFHT) in the infrared K band. The observations show at least three brightness peaks within the ring of lensed images, which we identify as emission from multiple lensing galaxies. The results confirm the suspected compound nature of the lens, as deduced from preliminary mass modeling. The detection of several additional nearby galaxies suggests that B1359+154 is lensed by the compact core of a small galaxy group. We attempted to produce an updated lens model based on the CFHT observations and new 5 GHz radio data obtained with the MERLIN array, but there are too few constraints to construct a realistic model at this time. The uncertainties inherent with modeling compound lenses make B1359+154 a challenging target for Hubble constant determination through the measurement of differential time delays. However, time delays will offer additional constraints to help pin down the mass model. This lens system therefore presents a unique opportunity to directly measure the mass distribution of a galaxy group at intermediate redshift.

  10. Adaptive Optics Imaging of the CLASS Gravitational Lens System B1359+154 with the Canada-France-Hawaii Telescope.

    PubMed

    Rusin; Hall; Nichol; Marlow; Richards; Myers

    2000-04-20

    We present adaptive optics imaging of the CLASS gravitational lens system B1359+154 obtained with the Canada-France-Hawaii Telescope (CFHT) in the infrared K band. The observations show at least three brightness peaks within the ring of lensed images, which we identify as emission from multiple lensing galaxies. The results confirm the suspected compound nature of the lens, as deduced from preliminary mass modeling. The detection of several additional nearby galaxies suggests that B1359+154 is lensed by the compact core of a small galaxy group. We attempted to produce an updated lens model based on the CFHT observations and new 5 GHz radio data obtained with the MERLIN array, but there are too few constraints to construct a realistic model at this time. The uncertainties inherent with modeling compound lenses make B1359+154 a challenging target for Hubble constant determination through the measurement of differential time delays. However, time delays will offer additional constraints to help pin down the mass model. This lens system therefore presents a unique opportunity to directly measure the mass distribution of a galaxy group at intermediate redshift. PMID:10770697

  11. THE FIRST CIRCUMSTELLAR DISK IMAGED IN SILHOUETTE AT VISIBLE WAVELENGTHS WITH ADAPTIVE OPTICS: MagAO IMAGING OF ORION 218-354

    SciTech Connect

    Follette, Katherine B.; Close, Laird M.; Males, Jared R.; Wu, Ya-Lin; Morzinski, Katie M.; Hinz, Philip; Rodigas, Timothy J.; Kopon, Derek; Puglisi, Alfio; Esposito, Simone; Riccardi, Armando; Pinna, Enrico; Xompero, Marco; Briguglio, Runa

    2013-09-20

    We present high-resolution adaptive optics (AO) corrected images of the silhouette disk Orion 218-354 taken with Magellan AO (MagAO) and its visible light camera, VisAO, in simultaneous differential imaging mode at Hα. This is the first image of a circumstellar disk seen in silhouette with AO and is among the first visible light AO results in the literature. We derive the disk extent, geometry, intensity, and extinction profiles and find, in contrast with previous work, that the disk is likely optically thin at Hα. Our data provide an estimate of the column density in primitive, ISM-like grains as a function of radius in the disk. We estimate that only ∼10% of the total submillimeter derived disk mass lies in primitive, unprocessed grains. We use our data, Monte Carlo radiative transfer modeling, and previous results from the literature to make the first self-consistent multiwavelength model of Orion 218-354. We find that we are able to reproduce the 1-1000 μm spectral energy distribution with a ∼2-540 AU disk of the size, geometry, small versus large grain proportion, and radial mass profile indicated by our data. This inner radius is a factor of ∼15 larger than the sublimation radius of the disk, suggesting that it is likely cleared in the very interior.

  12. Adaptive optics applications in vision science

    NASA Astrophysics Data System (ADS)

    Olivier, Scot S.

    2003-06-01

    Adaptive optics can be used to correct the aberrations in the human eye caused by imperfections in the cornea and the lens and thereby, improve image quality both looking into and out of the eye. Under the auspices of the NSF Center for Adaptive Optics and the DOE Biomedical Engineering Program, Lawrence Livermore National Laboratory has joined together with leading vision science researchers around the country to develop and test new ophthalmic imaging systems using novel wavefront corrector technologies. Results of preliminary comparative evaluations of these technologies in initial system tests show promise for future clinical utility.

  13. Adaptive Optics Applications in Vision Science

    SciTech Connect

    Olivier, S S

    2003-03-17

    Adaptive optics can be used to correct the aberrations in the human eye caused by imperfections in the cornea and the lens and thereby, improve image quality both looking into and out of the eye. Under the auspices of the NSF Center for Adaptive Optics and the DOE Biomedical Engineering Program, Lawrence Livermore National Laboratory has joined together with leading vision science researchers around the country to develop and test new ophthalmic imaging systems using novel wavefront corrector technologies. Results of preliminary comparative evaluations of these technologies in initial system tests show promise for future clinical utility.

  14. ADAPTIVE OPTICS IMAGING OF QUASI-STELLAR OBJECTS WITH DOUBLE-PEAKED NARROW LINES: ARE THEY DUAL ACTIVE GALACTIC NUCLEI?

    SciTech Connect

    Rosario, D. J.; McGurk, R. C.; Max, C. E.; Shields, G. A.; Smith, K. L.; Ammons, S. M. E-mail: mcgurk@ucsc.edu E-mail: shieldsga@mail.utexas.edu E-mail: ammons@as.arizona.edu

    2011-09-20

    Active galaxies hosting two accreting and merging supermassive black holes (SMBHs)-dual active galactic nuclei (AGNs)-are predicted by many current and popular models of black-hole-galaxy co-evolution. We present here the results of a program that has identified a set of probable dual AGN candidates based on near-infrared laser guide star adaptive optics imaging with the Keck II telescope. These candidates are selected from a complete sample of radio-quiet quasi-stellar objects (QSOs) drawn from the Sloan Digital Sky Survey (SDSS), which show double-peaked narrow AGN emission lines. Of the 12 AGNs imaged, we find 6 with double galaxy structure, of which four are in galaxy mergers. We measure the ionization of the two velocity components in the narrow AGN lines to test the hypothesis that both velocity components come from an active nucleus. The combination of a well-defined parent sample and high-quality imaging allows us to place constraints on the fraction of SDSS QSOs that host dual accreting black holes separated on kiloparsec scales: {approx}0.3%-0.65%. We derive from this fraction the time spent in a QSO phase during a typical merger and find a value that is much lower than estimates that arise from QSO space densities and galaxy merger statistics. We discuss possible reasons for this difference. Finally, we compare the SMBH mass distributions of single and dual AGNs and find little difference between the two within the limited statistics of our program, hinting that most SMBH growth happens in the later stages of a merger process.

  15. Adaptive Image Denoising by Mixture Adaptation

    NASA Astrophysics Data System (ADS)

    Luo, Enming; Chan, Stanley H.; Nguyen, Truong Q.

    2016-10-01

    We propose an adaptive learning procedure to learn patch-based image priors for image denoising. The new algorithm, called the Expectation-Maximization (EM) adaptation, takes a generic prior learned from a generic external database and adapts it to the noisy image to generate a specific prior. Different from existing methods that combine internal and external statistics in ad-hoc ways, the proposed algorithm is rigorously derived from a Bayesian hyper-prior perspective. There are two contributions of this paper: First, we provide full derivation of the EM adaptation algorithm and demonstrate methods to improve the computational complexity. Second, in the absence of the latent clean image, we show how EM adaptation can be modified based on pre-filtering. Experimental results show that the proposed adaptation algorithm yields consistently better denoising results than the one without adaptation and is superior to several state-of-the-art algorithms.

  16. Adaptive Optics Communications Performance Analysis

    NASA Technical Reports Server (NTRS)

    Srinivasan, M.; Vilnrotter, V.; Troy, M.; Wilson, K.

    2004-01-01

    The performance improvement obtained through the use of adaptive optics for deep-space communications in the presence of atmospheric turbulence is analyzed. Using simulated focal-plane signal-intensity distributions, uncoded pulse-position modulation (PPM) bit-error probabilities are calculated assuming the use of an adaptive focal-plane detector array as well as an adaptively sized single detector. It is demonstrated that current practical adaptive optics systems can yield performance gains over an uncompensated system ranging from approximately 1 dB to 6 dB depending upon the PPM order and background radiation level.

  17. Progress on Developing Adaptive Optics–Optical Coherence Tomography for In Vivo Retinal Imaging: Monitoring and Correction of Eye Motion Artifacts

    PubMed Central

    Zawadzki, Robert J.; Capps, Arlie G.; Kim, Dae Yu; Panorgias, Athanasios; Stevenson, Scott B.; Hamann, Bernd; Werner, John S.

    2014-01-01

    Recent progress in retinal image acquisition techniques, including optical coherence tomography (OCT) and scanning laser ophthalmoscopy (SLO), combined with improved performance of adaptive optics (AO) instrumentation, has resulted in improvement in the quality of in vivo images of cellular structures in the human retina. Here, we present a short review of progress on developing AO-OCT instruments. Despite significant progress in imaging speed and resolution, eye movements present during acquisition of a retinal image with OCT introduce motion artifacts into the image, complicating analysis and registration. This effect is especially pronounced in high-resolution datasets acquired with AO-OCT instruments. Several retinal tracking systems have been introduced to correct retinal motion during data acquisition. We present a method for correcting motion artifacts in AO-OCT volume data after acquisition using simultaneously captured adaptive optics-scanning laser ophthalmoscope (AO-SLO) images. We extract transverse eye motion data from the AO-SLO images, assign a motion adjustment vector to each AO-OCT A-scan, and re-sample from the scattered data back onto a regular grid. The corrected volume data improve the accuracy of quantitative analyses of microscopic structures. PMID:25544826

  18. Adaptive Optics at Lawrence Livermore National Laboratory

    SciTech Connect

    Gavel, D T

    2003-03-10

    Adaptive optics enables high resolution imaging through the atmospheric by correcting for the turbulent air's aberrations to the light waves passing through it. The Lawrence Livermore National Laboratory for a number of years has been at the forefront of applying adaptive optics technology to astronomy on the world's largest astronomical telescopes, in particular at the Keck 10-meter telescope on Mauna Kea, Hawaii. The technology includes the development of high-speed electrically driven deformable mirrors, high-speed low-noise CCD sensors, and real-time wavefront reconstruction and control hardware. Adaptive optics finds applications in many other areas where light beams pass through aberrating media and must be corrected to maintain diffraction-limited performance. We describe systems and results in astronomy, medicine (vision science), and horizontal path imaging, all active programs in our group.

  19. Adaptive-optics performance of Antarctic telescopes.

    PubMed

    Lawrence, Jon S

    2004-02-20

    The performance of natural guide star adaptive-optics systems for telescopes located on the Antarctic plateau is evaluated and compared with adaptive-optics systems operated with the characteristic mid-latitude atmosphere found at Mauna Kea. A 2-m telescope with tip-tilt correction and an 8-m telescope equipped with a high-order adaptive-optics system are considered. Because of the large isoplanatic angle of the South Pole atmosphere, the anisoplanatic error associated with an adaptive-optics correction is negligible, and the achievable resolution is determined only by the fitting error associated with the number of corrected wave-front modes, which depends on the number of actuators on the deformable mirror. The usable field of view of an adaptive-optics equipped Antarctic telescope is thus orders of magnitude larger than for a similar telescope located at a mid-latitude site; this large field of view obviates the necessity for multiconjugate adaptive-optics systems that use multiple laser guide stars. These results, combined with the low infrared sky backgrounds, indicate that the Antarctic plateau is the best site on Earth at which to perform high-resolution imaging with large telescopes, either over large fields of view or with appreciable sky coverage. Preliminary site-testing results obtained recently from the Dome Concordia station indicate that this site is far superior to even the South Pole. PMID:15008551

  20. Robo-AO Kepler Planetary Candidate Survey. II. Adaptive Optics Imaging of 969 Kepler Exoplanet Candidate Host Stars

    NASA Astrophysics Data System (ADS)

    Baranec, Christoph; Ziegler, Carl; Law, Nicholas M.; Morton, Tim; Riddle, Reed; Atkinson, Dani; Schonhut, Jessica; Crepp, Justin

    2016-07-01

    We initiated the Robo-AO Kepler Planetary Candidate Survey in 2012 to observe each Kepler exoplanet candidate host star with high angular resolution, visible light, laser adaptive optics (AOs) imaging. Our goal is to find nearby stars lying in Kepler's photometric apertures that are responsible for the relatively high probability of false-positive exoplanet detections and that cause underestimates of the size of transit radii. Our comprehensive survey will also shed light on the effects of stellar multiplicity on exoplanet properties and will identify rare exoplanetary architectures. In this second part of our ongoing survey, we observed an additional 969 Kepler planet candidate hosts and we report blended stellar companions up to {{Δ }}m≈ 6 that contribute to Kepler's measured light curves. We found 203 companions within ˜4″ of 181 of the Kepler stars, of which 141 are new discoveries. We measure the nearby star probability for this sample of Kepler planet candidate host stars to be 10.6% ± 1.1% at angular separations up to 2.″5, significantly higher than the 7.4% ± 1.0% probability discovered in our initial sample of 715 stars; we find the probability increases to 17.6% ± 1.5% out to a separation of 4.″0. The median position of Kepler Objects of Interest (KOIs) observed in this survey are 1.°1 closer to the galactic plane, which may account for some of the nearby star probability enhancement. We additionally detail 50 Keck AO images of Robo-AO observed KOIs in order to confirm 37 companions detected at a <5σ significance level and to obtain additional infrared photometry on higher significance detected companions.

  1. Adaptive optical filter

    DOEpatents

    Whittemore, Stephen Richard

    2013-09-10

    Imaging systems include a detector and a spatial light modulator (SLM) that is coupled so as to control image intensity at the detector based on predetermined detector limits. By iteratively adjusting SLM element values, image intensity at one or all detector elements or portions of an imaging detector can be controlled to be within limits. The SLM can be secured to the detector at a spacing such that the SLM is effectively at an image focal plane. In some applications, the SLM can be adjusted to impart visible or hidden watermarks to images or to reduce image intensity at one or a selected set of detector elements so as to reduce detector blooming

  2. Optical image encryption topology.

    PubMed

    Yong-Liang, Xiao; Xin, Zhou; Qiong-Hua, Wang; Sheng, Yuan; Yao-Yao, Chen

    2009-10-15

    Optical image encryption topology is proposed based on the principle of random-phase encoding. Various encryption topological units, involving peer-to-peer, ring, star, and tree topologies, can be realized by an optical 6f system. These topological units can be interconnected to constitute an optical image encryption network. The encryption and decryption can be performed in both digital and optical methods.

  3. Robust Wiener filtering for Adaptive Optics

    SciTech Connect

    Poyneer, L A

    2004-06-17

    In many applications of optical systems, the observed field in the pupil plane has a non-uniform phase component. This deviation of the phase of the field from uniform is called a phase aberration. In imaging systems this aberration will degrade the quality of the images. In the case of a large astronomical telescope, random fluctuations in the atmosphere lead to significant distortion. These time-varying distortions can be corrected using an Adaptive Optics (AO) system, which is a real-time control system composed of optical, mechanical and computational parts. Adaptive optics is also applicable to problems in vision science, laser propagation and communication. For a high-level overview, consult this web site. For an in-depth treatment of the astronomical case, consult these books.

  4. Lens based adaptive optics scanning laser ophthalmoscope.

    PubMed

    Felberer, Franz; Kroisamer, Julia-Sophie; Hitzenberger, Christoph K; Pircher, Michael

    2012-07-30

    We present an alternative approach for an adaptive optics scanning laser ophthalmoscope (AO-SLO). In contrast to other commonly used AO-SLO instruments, the imaging optics consist of lenses. Images of the fovea region of 5 healthy volunteers are recorded. The system is capable to resolve human foveal cones in 3 out of 5 healthy volunteers. Additionally, we investigated the capability of the system to support larger scanning angles (up to 5°) on the retina. Finally, in order to demonstrate the performance of the instrument images of rod photoreceptors are presented.

  5. What Is Optical Imaging?

    ERIC Educational Resources Information Center

    Hespos, Susan J.

    2010-01-01

    This article introduces a promising new methodology called optical imaging. Optical imaging is used for measuring changes in cortical blood flow due to functional activation. The article outlines the pros and cons of using optical imaging for studying the brain correlates of perceptual, cognitive, and language development in infants and young…

  6. SEEDS ADAPTIVE OPTICS IMAGING OF THE ASYMMETRIC TRANSITION DISK OPH IRS 48 IN SCATTERED LIGHT

    SciTech Connect

    Follette, Katherine B.; Close, Laird M.; Grady, Carol A.; Swearingen, Jeremy R.; Sitko, Michael L.; Champney, Elizabeth H.; Van der Marel, Nienke; Maaskant, Koen; Min, Michiel; Takami, Michihiro; Kuchner, Marc J; McElwain, Michael W.; Muto, Takayuki; Mayama, Satoshi; Fukagawa, Misato; Russell, Ray W.; Kudo, Tomoyuki; Kusakabe, Nobuhiko; Hashimoto, Jun; Abe, Lyu; and others

    2015-01-10

    We present the first resolved near-infrared imagery of the transition disk Oph IRS 48 (WLY 2-48), which was recently observed with ALMA to have a strongly asymmetric submillimeter flux distribution. H-band polarized intensity images show a ∼60 AU radius scattered light cavity with two pronounced arcs of emission, one from northeast to southeast and one smaller, fainter, and more distant arc in the northwest. K-band scattered light imagery reveals a similar morphology, but with a clear third arc along the southwestern rim of the disk cavity. This arc meets the northwestern arc at nearly a right angle, revealing the presence of a spiral arm or local surface brightness deficit in the disk, and explaining the east-west brightness asymmetry in the H-band data. We also present 0.8-5.4 μm IRTF SpeX spectra of this object, which allow us to constrain the spectral class to A0 ± 1 and measure a low mass accretion rate of 10{sup –8.5} M {sub ☉} yr{sup –1}, both consistent with previous estimates. We investigate a variety of reddening laws in order to fit the multiwavelength spectral energy distribution of Oph IRS 48 and find a best fit consistent with a younger, higher luminosity star than previous estimates.

  7. In Vivo Adaptive Optics Imaging of the Temporal Raphe and Its Relationship to the Optic Disc and Fovea in the Human Retina

    PubMed Central

    Huang, Gang; Gast, Thomas J.; Burns, Stephen A.

    2014-01-01

    Purpose. To investigate the anatomy of the temporal raphe and its angular relationship to the optic disc and fovea in the human retina in vivo. Methods. Adaptive optics scanning laser ophthalmoscope (AOSLO) was used to image the temporal raphe in 11 young subjects. The raphe's angle relative to a horizontal line and the raphe-fovea-disc angle (angle between the raphe and the line connecting the disc and fovea center) were determined. In addition, to investigate the impact of aging on the raphe, we imaged the raphe at 9° eccentricity in 10 additional older healthy subjects and compared the raphe's anatomy between the two age groups. Results. The raphe's in vivo appearance was generally in agreement with major findings of ex vivo studies. The raphe angle was −1.67° ± 4.8°, with the ranges from −9° to 6°. It was related to the angle of the foveal depression relative to the disc. The raphe-fovea-disc angle was 170.3° ± 3.6°. The raphe gap, defined as the averaged distance between superior and inferior bundles, was significantly larger in the older subjects than in younger subjects (230.83 ± 113.22 μm vs. 1.93 ± 68.73 μm, P < 0.0001). Conclusions. The angle of the raphe in the study was not consistent with classic raphe models. While the angle showed relatively large individual variability, there seems to be a systematic relation between the disc, fovea, and raphe. It may be useful for individualizing retinal measurement strategies with regard to perimetry. PMID:25146991

  8. HIGH-EFFICIENCY AUTONOMOUS LASER ADAPTIVE OPTICS

    SciTech Connect

    Baranec, Christoph; Riddle, Reed; Tendulkar, Shriharsh; Hogstrom, Kristina; Bui, Khanh; Dekany, Richard; Kulkarni, Shrinivas; Law, Nicholas M.; Ramaprakash, A. N.; Burse, Mahesh; Chordia, Pravin; Das, Hillol; Punnadi, Sujit

    2014-07-20

    As new large-scale astronomical surveys greatly increase the number of objects targeted and discoveries made, the requirement for efficient follow-up observations is crucial. Adaptive optics imaging, which compensates for the image-blurring effects of Earth's turbulent atmosphere, is essential for these surveys, but the scarcity, complexity and high demand of current systems limit their availability for following up large numbers of targets. To address this need, we have engineered and implemented Robo-AO, a fully autonomous laser adaptive optics and imaging system that routinely images over 200 objects per night with an acuity 10 times sharper at visible wavelengths than typically possible from the ground. By greatly improving the angular resolution, sensitivity, and efficiency of 1-3 m class telescopes, we have eliminated a major obstacle in the follow-up of the discoveries from current and future large astronomical surveys.

  9. Adaptive Optics for Industry and Medicine

    NASA Astrophysics Data System (ADS)

    Dainty, Christopher

    2008-01-01

    pt. 1. Wavefront correctors and control. Liquid crystal lenses for correction of presbyopia (Invited Paper) / Guoqiang Li and Nasser Peyghambarian. Converging and diverging liquid crystal lenses (oral paper) / Andrew X. Kirby, Philip J. W. Hands, and Gordon D. Love. Liquid lens technology for miniature imaging systems: status of the technology, performance of existing products and future trends (invited paper) / Bruno Berge. Carbon fiber reinforced polymer deformable mirrors for high energy laser applications (oral paper) / S. R. Restaino ... [et al.]. Tiny multilayer deformable mirrors (oral paper) / Tatiana Cherezova ... [et al.]. Performance analysis of piezoelectric deformable mirrors (oral paper) / Oleg Soloviev, Mikhail Loktev and Gleb Vdovin. Deformable membrane mirror with high actuator density and distributed control (oral paper) / Roger Hamelinck ... [et al.]. Characterization and closed-loop demonstration of a novel electrostatic membrane mirror using COTS membranes (oral paper) / David Dayton ... [et al.]. Electrostatic micro-deformable mirror based on polymer materials (oral paper) / Frederic Zamkotsian ... [et al.]. Recent progress in CMOS integrated MEMS A0 mirror development (oral paper) / A. Gehner ... [et al.]. Compact large-stroke piston-tip-tilt actuator and mirror (oral paper) / W. Noell ... [et al.]. MEMS deformable mirrors for high performance AO applications (oral paper) / Paul Bierden, Thomas Bifano and Steven Cornelissen. A versatile interferometric test-rig for the investigation and evaluation of ophthalmic AO systems (poster paper) / Steve Gruppetta, Jiang Jian Zhong and Luis Diaz-Santana. Woofer-tweeter adaptive optics (poster paper) / Thomas Farrell and Chris Dainty. Deformable mirrors based on transversal piezoeffect (poster paper) / Gleb Vdovin, Mikhail Loktev and Oleg Soloviev. Low-cost spatial light modulators for ophthalmic applications (poster paper) / Vincente Durán ... [et al.]. Latest MEMS DM developments and the path ahead

  10. Wavefront sensorless adaptive optics ophthalmoscopy in the human eye.

    PubMed

    Hofer, Heidi; Sredar, Nripun; Queener, Hope; Li, Chaohong; Porter, Jason

    2011-07-18

    Wavefront sensor noise and fidelity place a fundamental limit on achievable image quality in current adaptive optics ophthalmoscopes. Additionally, the wavefront sensor 'beacon' can interfere with visual experiments. We demonstrate real-time (25 Hz), wavefront sensorless adaptive optics imaging in the living human eye with image quality rivaling that of wavefront sensor based control in the same system. A stochastic parallel gradient descent algorithm directly optimized the mean intensity in retinal image frames acquired with a confocal adaptive optics scanning laser ophthalmoscope (AOSLO). When imaging through natural, undilated pupils, both control methods resulted in comparable mean image intensities. However, when imaging through dilated pupils, image intensity was generally higher following wavefront sensor-based control. Despite the typically reduced intensity, image contrast was higher, on average, with sensorless control. Wavefront sensorless control is a viable option for imaging the living human eye and future refinements of this technique may result in even greater optical gains.

  11. The Absolute Age of the Globular Cluster M15 Using Near-infrared Adaptive Optics Images from PISCES/LBT.

    NASA Astrophysics Data System (ADS)

    Monelli, M.; Testa, V.; Bono, G.; Ferraro, I.; Iannicola, G.; Fiorentino, G.; Arcidiacono, C.; Massari, D.; Boutsia, K.; Briguglio, R.; Busoni, L.; Carini, R.; Close, L.; Cresci, G.; Esposito, S.; Fini, L.; Fumana, M.; Guerra, J. C.; Hill, J.; Kulesa, C.; Mannucci, F.; McCarthy, D.; Pinna, E.; Puglisi, A.; Quiros-Pacheco, F.; Ragazzoni, R.; Riccardi, A.; Skemer, A.; Xompero, M.

    2015-10-01

    We present deep near-infrared J, {K}{{s}} photometry of the old, metal-poor Galactic globular cluster M15 obtained with images collected with the LUCI1 and PISCES cameras available at the Large Binocular Telescope (LBT). We show how the use of First Light Adaptive Optics (FLAO) system coupled with the PISCES camera allows us to improve the limiting magnitude by ˜2 mag in {K}{{s}}. By analyzing archival Hubble Space Telescope data, we demonstrate that the quality of the LBT/PISCES color-magnitude diagram is fully comparable with analogous space-based data. The smaller field of view is balanced by the shorter exposure time required to reach a similar photometric limit. We investigated the absolute age of M15 by means of two methods: (i) by determining the age from the position of the main-sequence turnoff (MSTO), and (ii) by the magnitude difference between the MSTO and the well-defined knee detected along the faint portion of the MS. We derive consistent values of the absolute age of M15, that is, 12.9 ± 2.6 Gyr and 13.3 ± 1.1 Gyr, respectively. Observations were carried out using the Large Binocular Telescope at Mount Graham, AZ. The LBT is an international collaboration among institutions in the United States, Italy, and Germany. LBT Corporation partners are the University of Arizona on behalf of the Arizona university system; Istituto Nazionale di Astrofisica, Italy; LBT Beteiligungsgesellschaft, Germany, representing the Max-Planck Society, the Astrophysical Institute Potsdam, and Heidelberg University; the Ohio State University; and the Research Corporation, on behalf of the University of Notre Dame, University of Minnesota, and University of Virginia.

  12. Compressive Optical Image Encryption

    PubMed Central

    Li, Jun; Sheng Li, Jiao; Yang Pan, Yang; Li, Rong

    2015-01-01

    An optical image encryption technique based on compressive sensing using fully optical means has been proposed. An object image is first encrypted to a white-sense stationary noise pattern using a double random phase encoding (DRPE) method in a Mach-Zehnder interferometer. Then, the encrypted image is highly compressed to a signal using single-pixel compressive holographic imaging in the optical domain. At the receiving terminal, the encrypted image is reconstructed well via compressive sensing theory, and the original image can be decrypted with three reconstructed holograms and the correct keys. The numerical simulations show that the method is effective and suitable for optical image security transmission in future all-optical networks because of the ability of completely optical implementation and substantially smaller hologram data volume. PMID:25992946

  13. Compressive Optical Image Encryption

    NASA Astrophysics Data System (ADS)

    Li, Jun; Sheng Li, Jiao; Yang Pan, Yang; Li, Rong

    2015-05-01

    An optical image encryption technique based on compressive sensing using fully optical means has been proposed. An object image is first encrypted to a white-sense stationary noise pattern using a double random phase encoding (DRPE) method in a Mach-Zehnder interferometer. Then, the encrypted image is highly compressed to a signal using single-pixel compressive holographic imaging in the optical domain. At the receiving terminal, the encrypted image is reconstructed well via compressive sensing theory, and the original image can be decrypted with three reconstructed holograms and the correct keys. The numerical simulations show that the method is effective and suitable for optical image security transmission in future all-optical networks because of the ability of completely optical implementation and substantially smaller hologram data volume.

  14. High-Resolution Imaging of Parafoveal Cones in Different Stages of Diabetic Retinopathy Using Adaptive Optics Fundus Camera

    PubMed Central

    Soliman, Mohamed Kamel; Hassan, Muhammad; Hanout, Mostafa; Graf, Frank; High, Robin; Do, Diana V.; Nguyen, Quan Dong; Sepah, Yasir J.

    2016-01-01

    Purpose To assess cone density as a marker of early signs of retinopathy in patients with type II diabetes mellitus. Methods An adaptive optics (AO) retinal camera (rtx1™; Imagine Eyes, Orsay, France) was used to acquire images of parafoveal cones from patients with type II diabetes mellitus with or without retinopathy and from healthy controls with no known systemic or ocular disease. Cone mosaic was captured at 0° and 2°eccentricities along the horizontal and vertical meridians. The density of the parafoveal cones was calculated within 100×100-μm squares located at 500-μm from the foveal center along the orthogonal meridians. Manual corrections of the automated counting were then performed by 2 masked graders. Cone density measurements were evaluated with ANOVA that consisted of one between-subjects factor, stage of retinopathy and the within-subject factors. The ANOVA model included a complex covariance structure to account for correlations between the levels of the within-subject factors. Results Ten healthy participants (20 eyes) and 25 patients (29 eyes) with type II diabetes mellitus were recruited in the study. The mean (± standard deviation [SD]) age of the healthy participants (Control group), patients with diabetes without retinopathy (No DR group), and patients with diabetic retinopathy (DR group) was 55 ± 8, 53 ± 8, and 52 ± 9 years, respectively. The cone density was significantly lower in the moderate nonproliferative diabetic retinopathy (NPDR) and severe NPDR/proliferative DR groups compared to the Control, No DR, and mild NPDR groups (P < 0.05). No correlation was found between cone density and the level of hemoglobin A1c (HbA1c) or the duration of diabetes. Conclusions The extent of photoreceptor loss on AO imaging may correlate positively with severity of DR in patients with type II diabetes mellitus. Photoreceptor loss may be more pronounced among patients with advanced stages of DR due to higher risk of macular edema and its

  15. Adaptive optics and laser guide stars at Lick observatory

    SciTech Connect

    Brase, J.M.

    1994-11-15

    For the past several years LLNL has been developing adaptive optics systems for correction of both atmospheric turbulence effects and thermal distortions in optics for high-power lasers. Our early work focused on adaptive optics for beam control in laser isotope separation and ground-based free electron lasers. We are currently developing innovative adaptive optics and laser systems for sodium laser guide star applications at the University of California`s Lick and Keck Observeratories. This talk will describe our adaptive optics technology and some of its applications in high-resolution imaging and beam control.

  16. Adaptive optics without guide stars (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Mertz, Jerome; Li, Jiang; Beaulieu, Devin; Paudel, Hari P.; Barankov, Roman; Bifano, Thomas G.

    2016-03-01

    Adaptive optics is a strategy to compensate for sample-induced aberrations in microscopy applications. Generally, it requires the presence of "guide stars" in the sample to serve as localized reference targets. We describe an implementation of conjugate adaptive optics that is amenable to widefield (i.e. non-scanning) microscopy, and can provide aberration corrections over potentially large fields of view without the use of guide stars. A unique feature of our implementation is that it is based on wavefront sensing with a single-shot partitioned-aperture sensor that provides large dynamic range compatible with extended samples. Combined information provided by this sensor and the imaging camera enable robust image de-blurring based on a rapid estimation of sample and aberrations obtained by closed-loop feedback. We present the theoretical principle of our technique and experimental demonstrations using both trans-illumination and fluorescence microscopes. Finally, we apply our technique to mouse brain imaging.

  17. Adaptive optics parallel near-confocal scanning ophthalmoscopy.

    PubMed

    Lu, Jing; Gu, Boyu; Wang, Xiaolin; Zhang, Yuhua

    2016-08-15

    We present an adaptive optics parallel near-confocal scanning ophthalmoscope (AOPCSO) using a digital micromirror device (DMD). The imaging light is modulated to be a line of point sources by the DMD, illuminating the retina simultaneously. By using a high-speed line camera to acquire the image and using adaptive optics to compensate the ocular wave aberration, the AOPCSO can image the living human eye with cellular level resolution at the frame rate of 100 Hz. AOPCSO has been demonstrated with improved spatial resolution in imaging of the living human retina compared with adaptive optics line scan ophthalmoscopy.

  18. Adaptive optics parallel near-confocal scanning ophthalmoscopy.

    PubMed

    Lu, Jing; Gu, Boyu; Wang, Xiaolin; Zhang, Yuhua

    2016-08-15

    We present an adaptive optics parallel near-confocal scanning ophthalmoscope (AOPCSO) using a digital micromirror device (DMD). The imaging light is modulated to be a line of point sources by the DMD, illuminating the retina simultaneously. By using a high-speed line camera to acquire the image and using adaptive optics to compensate the ocular wave aberration, the AOPCSO can image the living human eye with cellular level resolution at the frame rate of 100 Hz. AOPCSO has been demonstrated with improved spatial resolution in imaging of the living human retina compared with adaptive optics line scan ophthalmoscopy. PMID:27519106

  19. Split image optical display

    DOEpatents

    Veligdan, James T.

    2007-05-29

    A video image is displayed from an optical panel by splitting the image into a plurality of image components, and then projecting the image components through corresponding portions of the panel to collectively form the image. Depth of the display is correspondingly reduced.

  20. Split image optical display

    DOEpatents

    Veligdan, James T.

    2005-05-31

    A video image is displayed from an optical panel by splitting the image into a plurality of image components, and then projecting the image components through corresponding portions of the panel to collectively form the image. Depth of the display is correspondingly reduced.

  1. Fluorescent scanning laser ophthalmoscopy for cellular resolution in vivo mouse retinal imaging: benefits and drawbacks of implementing adaptive optics (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Zhang, Pengfei; Goswami, Mayank; Pugh, Edward N.; Zawadzki, Robert J.

    2016-03-01

    Scanning Laser Ophthalmoscopy (SLO) is a very important imaging tool in ophthalmology research. By combing with Adaptive Optics (AO) technique, AO-SLO can correct for ocular aberrations resulting in cellular level resolution, allowing longitudinal studies of single cells morphology in the living eyes. The numerical aperture (NA) sets the optical resolution that can be achieve in the "classical" imaging systems. Mouse eye has more than twice NA of the human eye, thus offering theoretically higher resolution. However, in most SLO based imaging systems the imaging beam size at mouse pupil sets the NA of that instrument, while most of the AO-SLO systems use almost the full NA of the mouse eye. In this report, we first simulated the theoretical resolution that can be achieved in vivo for different imaging beam sizes (different NA), assumingtwo cases: no aberrations and aberrations based on published mouse ocular wavefront data. Then we imaged mouse retinas with our custom build SLO system using different beam sizes to compare these results with theory. Further experiments include comparison of the SLO and AO-SLO systems for imaging different type of fluorescently labeled cells (microglia, ganglion, photoreceptors, etc.). By comparing those results and taking into account systems complexity and ease of use, the benefits and drawbacks of two imaging systems will be discussed.

  2. Performance of the optical communication adaptive optics testbed

    NASA Technical Reports Server (NTRS)

    Troy, Mitchell; Roberts, Jennifer; Guiwits, Steve; Azevedo, Steve; Bikkannavar, Siddarayappa; Brack, Gary; Garkanian, Vachik; Palmer, Dean; Platt, Benjamin; Truong, Tuan; Wilson, Keith; Wallace, Kent

    2005-01-01

    We describe the current performance of an adaptive optics testbed for optical communication. This adaptive optics system allows for simulation of night and day-time observing on a 1 meter telescope with a 97 actuator deformable mirror.

  3. 15 Gbit/s indoor optical wireless systems employing fast adaptation and imaging reception in a realistic environment

    NASA Astrophysics Data System (ADS)

    Alsaadi, Fuad E.

    2016-03-01

    Optical wireless systems are promising candidates for next-generation indoor communication networks. Optical wireless technology offers freedom from spectrum regulations and, compared to current radio-frequency networks, higher data rates and increased security. This paper presents a fast adaptation method for multibeam angle and delay adaptation systems and a new spot-diffusing geometry, and also considers restrictions needed for complying with eye safety regulations. The fast adaptation algorithm reduces the computational load required to reconfigure the transmitter in the case of transmitter and/or receiver mobility. The beam clustering approach enables the transmitter to assign power to spots within the pixel's field of view (FOV) and increases the number of such spots. Thus, if the power per spot is restricted to comply with eye safety standards, the new approach, in which more spots are visible within the FOV of the pixel, leads to enhanced signal-to-noise ratio (SNR). Simulation results demonstrate that the techniques proposed in this paper lead to SNR improvements that enable reliable operation at data rates as high as 15 Gbit/s. These results are based on simulation and not on actual measurements or experiments.

  4. Adaptive Optics for the Human Eye

    NASA Astrophysics Data System (ADS)

    Williams, D. R.

    2000-05-01

    Adaptive optics can extend not only the resolution of ground-based telescopes, but also the human eye. Both static and dynamic aberrations in the cornea and lens of the normal eye limit its optical quality. Though it is possible to correct defocus and astigmatism with spectacle lenses, higher order aberrations remain. These aberrations blur vision and prevent us from seeing at the fundamental limits set by the retina and brain. They also limit the resolution of cameras to image the living retina, cameras that are a critical for the diagnosis and treatment of retinal disease. I will describe an adaptive optics system that measures the wave aberration of the eye in real time and compensates for it with a deformable mirror, endowing the human eye with unprecedented optical quality. This instrument provides fresh insight into the ultimate limits on human visual acuity, reveals for the first time images of the retinal cone mosaic responsible for color vision, and points the way to contact lenses and laser surgical methods that could enhance vision beyond what is currently possible today. Supported by the NSF Science and Technology Center for Adaptive Optics, the National Eye Institute, and Bausch and Lomb, Inc.

  5. Comparison of the human multifocal electroretinogram a-wave response and adaptive optics imaging of cone photoreceptor numbers

    NASA Astrophysics Data System (ADS)

    Klein, Michael W.

    Studies that have used pharmacological agents in non human primates (e.g., Hood et al., IOVS 2002) indicate that electrical activity of cone photoreceptors, depolarizing cone bipolar cells and horizontal cells are all likely to contribute to the multifocal electroretinogram (mfERG) a-wave. The purpose of this study was to examine the relationship between the mfERG a-wave and co-localized cone spatial density individually measured in young healthy human subjects. mfERGs (0.1-300Hz) were recorded from 4 subjects (20-29 years) with a system from Veris Science (EDI, Inc.) using 2.4 degree unstretched hexagons from 206 retinal locations presented at 30 frames per m-step on a 75Hz monitor with m-sequence exponent of 9 and flash strength 9.9 cd-s/m 2. mfERG a-wave amplitude was measured from baseline at 10 milliseconds on the leading edge of the a-wave. In vivo cone images were obtained at 24 retinal locations using a custom-built Adaptive Optics Confocal Scanning Laser Ophthalmoscope. Cone spatial density was measured from a 100x100mum centered on the mfERG hexagons at 24 retinal locations. mfERG a-wave amplitude as well as cone density reduced with increase in retinal eccentricity from the fovea and the a-wave amplitude and cone density were positively correlated for each subject (r2=0.35 to 0.49 and p = 0.0049 to 0.0002). The coefficient of variation (CV) of the mfERG a-wave amplitude across subjects at each retinal location (16-62%) was larger than the CV of the cone density (8-37%) at the same location. The results indicate that underlying cone density accounts for a significant portion (up to nearly 70%) of the variance in the mfERG a-wave amplitude across retinal eccentricity. Other factors likely contribute to the variance (approximately 30%) of the measured mfERG parameters.

  6. Future trends in adaptive Optics

    NASA Astrophysics Data System (ADS)

    Le Louarn, Miska

    2001-05-01

    In this talk, I will summarize the limitations of current adaptive optics systems (cone effect, anisoplanatism) and I will show what methods can be used to overcome them. I will focus on Multi-Conjugate AO and the polychromatic laser guide star. I will also address AO for Extremely Large Telescopes (ELTs), such as OWL (ESO) and CELT (University of California / Caltech).

  7. Compact adaptive optics line scanning ophthalmoscope

    PubMed Central

    Mujat, Mircea; Ferguson, R. Daniel; Iftimia, Nicusor; Hammer, Daniel X.

    2010-01-01

    We have developed a compact retinal imager that integrates adaptive optics (AO) into a line scanning ophthalmoscope (LSO). The bench-top AO-LSO instrument significantly reduces the size, complexity, and cost of research AO scanning laser ophthalmoscopes (AOSLOs), for the purpose of moving adaptive optics imaging more rapidly into routine clinical use. The AO-LSO produces high resolution retinal images with only one moving part and a significantly reduced instrument footprint and number of optical components. The AO-LSO has a moderate field of view (5.5 deg), which allows montages of the macula or other targets to be obtained more quickly and efficiently. In a preliminary human subjects investigation, photoreceptors could be resolved and counted within ~0.5 mm of the fovea. Photoreceptor counts matched closely to previously reported histology. The capillaries surrounding the foveal avascular zone could be resolved, as well as cells flowing within them. Individual nerve fiber bundles could be resolved, especially near the optic nerve head, as well as other structures such as the lamina cribrosa. In addition to instrument design, fabrication, and testing, software algorithms were developed for automated image registration and cone counting. PMID:19506678

  8. Optical Property Analyses of Plant Cells for Adaptive Optics Microscopy

    NASA Astrophysics Data System (ADS)

    Tamada, Yosuke; Murata, Takashi; Hattori, Masayuki; Oya, Shin; Hayano, Yutaka; Kamei, Yasuhiro; Hasebe, Mitsuyasu

    2014-04-01

    In astronomy, adaptive optics (AO) can be used to cancel aberrations caused by atmospheric turbulence and to perform diffraction-limited observation of astronomical objects from the ground. AO can also be applied to microscopy, to cancel aberrations caused by cellular structures and to perform high-resolution live imaging. As a step toward the application of AO to microscopy, here we analyzed the optical properties of plant cells. We used leaves of the moss Physcomitrella patens, which have a single layer of cells and are thus suitable for optical analysis. Observation of the cells with bright field and phase contrast microscopy, and image degradation analysis using fluorescent beads demonstrated that chloroplasts provide the main source of optical degradations. Unexpectedly, the cell wall, which was thought to be a major obstacle, has only a minor effect. Such information provides the basis for the application of AO to microscopy for the observation of plant cells.

  9. Adaptive-optics SLO imaging combined with widefield OCT and SLO enables precise 3D localization of fluorescent cells in the mouse retina.

    PubMed

    Zawadzki, Robert J; Zhang, Pengfei; Zam, Azhar; Miller, Eric B; Goswami, Mayank; Wang, Xinlei; Jonnal, Ravi S; Lee, Sang-Hyuck; Kim, Dae Yu; Flannery, John G; Werner, John S; Burns, Marie E; Pugh, Edward N

    2015-06-01

    Adaptive optics scanning laser ophthalmoscopy (AO-SLO) has recently been used to achieve exquisite subcellular resolution imaging of the mouse retina. Wavefront sensing-based AO typically restricts the field of view to a few degrees of visual angle. As a consequence the relationship between AO-SLO data and larger scale retinal structures and cellular patterns can be difficult to assess. The retinal vasculature affords a large-scale 3D map on which cells and structures can be located during in vivo imaging. Phase-variance OCT (pv-OCT) can efficiently image the vasculature with near-infrared light in a label-free manner, allowing 3D vascular reconstruction with high precision. We combined widefield pv-OCT and SLO imaging with AO-SLO reflection and fluorescence imaging to localize two types of fluorescent cells within the retinal layers: GFP-expressing microglia, the resident macrophages of the retina, and GFP-expressing cone photoreceptor cells. We describe in detail a reflective afocal AO-SLO retinal imaging system designed for high resolution retinal imaging in mice. The optical performance of this instrument is compared to other state-of-the-art AO-based mouse retinal imaging systems. The spatial and temporal resolution of the new AO instrumentation was characterized with angiography of retinal capillaries, including blood-flow velocity analysis. Depth-resolved AO-SLO fluorescent images of microglia and cone photoreceptors are visualized in parallel with 469 nm and 663 nm reflectance images of the microvasculature and other structures. Additional applications of the new instrumentation are discussed.

  10. Adaptive-optics SLO imaging combined with widefield OCT and SLO enables precise 3D localization of fluorescent cells in the mouse retina

    PubMed Central

    Zawadzki, Robert J.; Zhang, Pengfei; Zam, Azhar; Miller, Eric B.; Goswami, Mayank; Wang, Xinlei; Jonnal, Ravi S.; Lee, Sang-Hyuck; Kim, Dae Yu; Flannery, John G.; Werner, John S.; Burns, Marie E.; Pugh, Edward N.

    2015-01-01

    Adaptive optics scanning laser ophthalmoscopy (AO-SLO) has recently been used to achieve exquisite subcellular resolution imaging of the mouse retina. Wavefront sensing-based AO typically restricts the field of view to a few degrees of visual angle. As a consequence the relationship between AO-SLO data and larger scale retinal structures and cellular patterns can be difficult to assess. The retinal vasculature affords a large-scale 3D map on which cells and structures can be located during in vivo imaging. Phase-variance OCT (pv-OCT) can efficiently image the vasculature with near-infrared light in a label-free manner, allowing 3D vascular reconstruction with high precision. We combined widefield pv-OCT and SLO imaging with AO-SLO reflection and fluorescence imaging to localize two types of fluorescent cells within the retinal layers: GFP-expressing microglia, the resident macrophages of the retina, and GFP-expressing cone photoreceptor cells. We describe in detail a reflective afocal AO-SLO retinal imaging system designed for high resolution retinal imaging in mice. The optical performance of this instrument is compared to other state-of-the-art AO-based mouse retinal imaging systems. The spatial and temporal resolution of the new AO instrumentation was characterized with angiography of retinal capillaries, including blood-flow velocity analysis. Depth-resolved AO-SLO fluorescent images of microglia and cone photoreceptors are visualized in parallel with 469 nm and 663 nm reflectance images of the microvasculature and other structures. Additional applications of the new instrumentation are discussed. PMID:26114038

  11. The research and development of the adaptive optics in ophthalmology

    NASA Astrophysics Data System (ADS)

    Wu, Chuhan; Zhang, Xiaofang; Chen, Weilin

    2015-08-01

    Recently the combination of adaptive optics and ophthalmology has made great progress and become highly effective. The retina disease is diagnosed by retina imaging technique based on scanning optical system, so the scanning of eye requires optical system characterized by great ability of anti-moving and optical aberration correction. The adaptive optics possesses high level of adaptability and is available for real time imaging, which meets the requirement of medical retina detection with accurate images. Now the Scanning Laser Ophthalmoscope and the Optical Coherence Tomography are widely used, which are the core techniques in the area of medical retina detection. Based on the above techniques, in China, a few adaptive optics systems used for eye medical scanning have been designed by some researchers from The Institute of Optics And Electronics of CAS(The Chinese Academy of Sciences); some foreign research institutions have adopted other methods to eliminate the interference of eye moving and optical aberration; there are many relevant patents at home and abroad. In this paper, the principles and relevant technique details of the Scanning Laser Ophthalmoscope and the Optical Coherence Tomography are described. And the recent development and progress of adaptive optics in the field of eye retina imaging are analyzed and summarized.

  12. Adaptive optics program at TMT

    NASA Astrophysics Data System (ADS)

    Boyer, C.; Adkins, Sean; Andersen, David R.; Atwood, Jenny; Bo, Yong; Byrnes, Peter; Caputa, Kris; Cavaco, Jeff; Ellerbroek, Brent; Gilles, Luc; Gregory, James; Herriot, Glen; Hickson, Paul; Ljusic, Zoran; Manter, Darren; Marois, Christian; Otárola, Angel; Pagès, Hubert; Schoeck, Matthias; Sinquin, Jean-Christophe; Smith, Malcolm; Spano, Paolo; Szeto, Kei; Tang, Jinlong; Travouillon, Tony; Véran, Jean-Pierre; Wang, Lianqi; Wei, Kai

    2014-07-01

    The TMT first light Adaptive Optics (AO) facility consists of the Narrow Field Infra-Red AO System (NFIRAOS) and the associated Laser Guide Star Facility (LGSF). NFIRAOS is a 60 × 60 laser guide star (LGS) multi-conjugate AO (MCAO) system, which provides uniform, diffraction-limited performance in the J, H, and K bands over 17-30 arc sec diameter fields with 50 per cent sky coverage at the galactic pole, as required to support the TMT science cases. NFIRAOS includes two deformable mirrors, six laser guide star wavefront sensors, and three low-order, infrared, natural guide star wavefront sensors within each client instrument. The first light LGSF system includes six sodium lasers required to generate the NFIRAOS laser guide stars. In this paper, we will provide an update on the progress in designing, modeling and validating the TMT first light AO systems and their components over the last two years. This will include pre-final design and prototyping activities for NFIRAOS, preliminary design and prototyping activities for the LGSF, design and prototyping for the deformable mirrors, fabrication and tests for the visible detectors, benchmarking and comparison of different algorithms and processing architecture for the Real Time Controller (RTC) and development and tests of prototype candidate lasers. Comprehensive and detailed AO modeling is continuing to support the design and development of the first light AO facility. Main modeling topics studied during the last two years include further studies in the area of wavefront error budget, sky coverage, high precision astrometry for the galactic center and other observations, high contrast imaging with NFIRAOS and its first light instruments, Point Spread Function (PSF) reconstruction for LGS MCAO, LGS photon return and sophisticated low order mode temporal filtering.

  13. Adaptive optics at the IOE, CAS

    NASA Astrophysics Data System (ADS)

    Jiang, Wenhan; Rao, Changhui; Zhang, Yudong; Ling, Ning; Guan, Chunlin

    2009-02-01

    R&D on Adaptive Optics in the Institute of Optics & Electronics (IOE), Chinese Academy of Sciences (CAS) began in 1979. In this paper, several recent achievements will be reported: 1. AO for astronomical telescopes. AO system for 1.2 m telescope at Yunnan Astronomical Observatory was built in 1998. It was updated in 2004, and high resolution images approaching diffraction limit were obtained. A new AO telescope with 1.8m aperture is being built, and a 4m AO telescope is being planned. 2. AO for ICF facility. A 19-element AO system with hill-climbing control algorithm for "Shenguang I" ICF facility was built in 1985, which was the first AO system used in ICF facility in the world. A set of 8 AO systems was installed in a bundle of ICF prototype for "Shenguang III" ICF facility. A new system is being built for further development of ICF facility. 3. AO for retinal imaging. In 1999, the first AO system with 19-element DM was built for retinal imaging. Several AO systems with 37-element deformable mirror (DM) were built and used for vision research and clinical inspection. It is being integrated with an OCT system for high resolution retinal imaging. All of the main subsystems, such as DMs, wavefront sensors, and high speed processors, were built in the Laboratory on Adaptive Optics of IOE, CAS.

  14. Is Linear Displacement Information Or Angular Displacement Information Used During The Adaptation of Pointing Responses To An Optically Shifted Image?

    NASA Technical Reports Server (NTRS)

    Bautista, Abigail B.

    1994-01-01

    Twenty-four observers looked through a pair of 20 diopter wedge prisms and pointed to an image of a target which was displaced vertically from eye level by 6 cm at a distance of 30 cm. Observers pointed 40 times, using only their right hand, and received error-corrective feedback upon termination of each pointing response (terminal visual feedback). At three testing distances, 20, 30, and 40 cm, ten pre-exposure and ten post-exposure pointing responses were recorded for each hand as observers reached to a mirror-viewed target located at eye level. The difference between pre- and post-exposure pointing response (adaptive shift) was compared for both Exposed and Unexposed hands across all three testing distances. The data were assessed according to the results predicted by two alternative models for processing spatial-information: one using angular displacement information and another using linear displacement information. The angular model of spatial mapping best predicted the observer's pointing response for the Exposed hand. Although the angular adaptive shift did not change significantly as a function of distance (F(2,44) = 1.12, n.s.), the linear adaptive shift increased significantly over the three testing distances 02 44) = 4.90 p less than 0.01).

  15. Characterization of Adaptive Optics at Keck Observatory

    SciTech Connect

    van Dam, M A; Macintosh, B A

    2003-07-24

    In this paper, the adaptive optics (AO) system at Keck Observatory is characterized. The AO system is described in detail. The physical parameters of the lenslets, CCD and deformable mirror, the calibration procedures and the signal processing algorithms are explained. Results of sky performance tests are presented: the AO system is shown to deliver images with an average Strehl ratio of up to 0.37 at 1.59 {micro}m using a bright guide star. An error budget that is consistent with the observed image quality is presented.

  16. Driver Code for Adaptive Optics

    NASA Technical Reports Server (NTRS)

    Rao, Shanti

    2007-01-01

    A special-purpose computer code for a deformable-mirror adaptive-optics control system transmits pixel-registered control from (1) a personal computer running software that generates the control data to (2) a circuit board with 128 digital-to-analog converters (DACs) that generate voltages to drive the deformable-mirror actuators. This program reads control-voltage codes from a text file, then sends them, via the computer s parallel port, to a circuit board with four AD5535 (or equivalent) chips. Whereas a similar prior computer program was capable of transmitting data to only one chip at a time, this program can send data to four chips simultaneously. This program is in the form of C-language code that can be compiled and linked into an adaptive-optics software system. The program as supplied includes source code for integration into the adaptive-optics software, documentation, and a component that provides a demonstration of loading DAC codes from a text file. On a standard Windows desktop computer, the software can update 128 channels in 10 ms. On Real-Time Linux with a digital I/O card, the software can update 1024 channels (8 boards in parallel) every 8 ms.

  17. Statistics of intensity in adaptive-optics images and their usefulness for detection and photometry of exoplanets.

    PubMed

    Gladysz, Szymon; Yaitskova, Natalia; Christou, Julian C

    2010-11-01

    This paper is an introduction to the problem of modeling the probability density function of adaptive-optics speckle. We show that with the modified Rician distribution one cannot describe the statistics of light on axis. A dual solution is proposed: the modified Rician distribution for off-axis speckle and gamma-based distribution for the core of the point spread function. From these two distributions we derive optimal statistical discriminators between real sources and quasi-static speckles. In the second part of the paper the morphological difference between the two probability density functions is used to constrain a one-dimensional, "blind," iterative deconvolution at the position of an exoplanet. Separation of the probability density functions of signal and speckle yields accurate differential photometry in our simulations of the SPHERE planet finder instrument.

  18. Adaptive optics scanning ophthalmoscopy with annular pupils.

    PubMed

    Sulai, Yusufu N; Dubra, Alfredo

    2012-07-01

    Annular apodization of the illumination and/or imaging pupils of an adaptive optics scanning light ophthalmoscope (AOSLO) for improving transverse resolution was evaluated using three different normalized inner radii (0.26, 0.39 and 0.52). In vivo imaging of the human photoreceptor mosaic at 0.5 and 10° from fixation indicates that the use of an annular illumination pupil and a circular imaging pupil provides the most benefit of all configurations when using a one Airy disk diameter pinhole, in agreement with the paraxial confocal microscopy theory. Annular illumination pupils with 0.26 and 0.39 normalized inner radii performed best in terms of the narrowing of the autocorrelation central lobe (between 7 and 12%), and the increase in manual and automated photoreceptor counts (8 to 20% more cones and 11 to 29% more rods). It was observed that the use of annular pupils with large inner radii can result in multi-modal cone photoreceptor intensity profiles. The effect of the annular masks on the average photoreceptor intensity is consistent with the Stiles-Crawford effect (SCE). This indicates that combinations of images of the same photoreceptors with different apodization configurations and/or annular masks can be used to distinguish cones from rods, even when the former have complex multi-modal intensity profiles. In addition to narrowing the point spread function transversally, the use of annular apodizing masks also elongates it axially, a fact that can be used for extending the depth of focus of techniques such as adaptive optics optical coherence tomography (AOOCT). Finally, the positive results from this work suggest that annular pupil apodization could be used in refractive or catadioptric adaptive optics ophthalmoscopes to mitigate undesired back-reflections.

  19. Gemini Frontier Fields: Wide-field Adaptive Optics Ks-band Imaging of the Galaxy Clusters MACS J0416.1-2403 and Abell 2744

    NASA Astrophysics Data System (ADS)

    Schirmer, M.; Carrasco, E. R.; Pessev, P.; Garrel, V.; Winge, C.; Neichel, B.; Vidal, F.

    2015-04-01

    We have observed two of the six Frontier Fields galaxy clusters, MACS J0416.1-2403 and Abell 2744, using the Gemini Multi-Conjugate Adaptive Optics System (GeMS) and the Gemini South Adaptive Optics Imager (GSAOI). With 0.″ 08-0.″ 10 FWHM our data are nearly diffraction-limited over a 100\\prime\\prime × 100\\prime\\prime wide area. GeMS/GSAOI complements the Hubble Space Telescope (HST) redwards of 1.6 μm with twice the angular resolution. We reach a 5σ depth of {{K}s}˜ 25.6 mag (AB) for compact sources. In this paper, we describe the observations, data processing, and initial public data release. We provide fully calibrated, co-added images matching the native GSAOI pixel scale as well as the larger plate scales of the HST release, adding to the legacy value of the Frontier Fields. Our work demonstrates that even for fields at high galactic latitude where natural guide stars are rare, current multi-conjugated adaptive optics technology at 8 m telescopes has opened a new window on the distant universe. Observations of a third Frontier Field, Abell 370, are planned. Based on observations obtained at the Gemini Observatory, which is operated by the Association of Universities for Research in Astronomy, Inc., under a cooperative agreement with the NSF on behalf of the Gemini partnership: the National Science Foundation (United States), the Science and Technology Facilities Council (United Kingdom), the National Research Council (Canada), CONICYT (Chile), the Australian Research Council (Australia), Ministério da Ciência, Tecnologia e Inovação (Brazil) and Ministerio de Ciencia, Tecnología e Innovación Productiva (Argentina). Based on observations made with ESO Telescopes at the La Silla and Paranal Observatories, Chile.

  20. Multimodal optical imaging.

    PubMed

    Lawler, Cindy; Suk, William A; Pitt, Bruce R; Croix, Claudette M St; Watkins, Simon C

    2003-08-01

    The recent resurgence of interest in the use of intravital microscopy in lung research is a manifestation of extraordinary progress in visual imaging and optical microscopy. This review evaluates the tools and instrumentation available for a number of imaging modalities, with particular attention to recent technological advances, and addresses recent progress in use of optical imaging techniques in basic pulmonary research.1 Limitations of existing methods and anticipated future developments are also identified. Although there have also been major advances made in the use of magnetic resonance imaging, positron emission tomography, and X-ray and computed tomography to image intact lungs and while these technologies have been instrumental in advancing the diagnosis and treatment of patients, the purpose of this review is to outline developing optical methods that can be evaluated for use in basic research in pulmonary biology.

  1. Scientific Objectives and Design Study of an Adaptive Optics Visual Echelle Spectrograph and Imager Coronograph (AVES-IMCO) for the NAOS Visitor Focus at the VLT

    NASA Astrophysics Data System (ADS)

    Pallavicini, Roberto; Zerbi, Filippo; Beuzit, Jean-Luc; Bonanno, Giovanni; Bonifacio, Piercarlo; Comari, Maurizio; Conconi, Paolo; Delabre, Bernard; Franchini, Mariagrazia; Marcantonio, Paolo Di; Lagrange, Anne-Marie; Mazzoleni, Ruben; Molaro, Paolo; Pasquini, Luca; Santin, Paolo

    We present the scientific case for an Adaptive Optics Visual Echelle Spectrograph and Imager Coronograph (AVES-IMCO) that we propose as a visitor instrument for the secondary port of NAOS at the VLT. We show that such an instrument would be ideal for intermediate resolution (R=16,000) spectroscopy of faint sky-limited objects down to a magnitude of V=24.0 and will complement very effectively the near-IR imaging capabilities of CONICA. We present examples of science programmes that could be carried out with such an instrument and which cannot be addressed with existing VLT instruments. We also report on the result of a two-year design study of the instrument, with specific reference to its use as parallel instrument of NAOS.

  2. Applications of Adaptive Optics Scanning Laser Ophthalmoscopy

    PubMed Central

    Roorda, Austin

    2010-01-01

    Adaptive optics (AO) describes a set of tools to correct or control aberrations in any optical system. In the eye, AO allows for precise control of the ocular aberrations. If used to correct aberrations over a large pupil, for example, cellular level resolution in retinal images can be achieved. AO systems have been demonstrated for advanced ophthalmoscopy as well as for testing and/or improving vision. In fact, AO can be integrated to any ophthalmic instrument where the optics of the eye is involved, with a scope of applications ranging from phoropters to optical coherence tomography systems. In this paper, I discuss the applications and advantages of using AO in a specific system, the adaptive optics scanning laser ophthalmoscope, or AOSLO. Since the Borish award was, in part, awarded to me because of this effort, I felt it appropriate to select this as the topic for this paper. Furthermore, users of AOSLO continue to appreciate the benefits of the technology, some of which were not anticipated at the time of development, and so it is time to revisit this topic and summarize them in a single paper. PMID:20160657

  3. Fast simulated annealing and adaptive Monte Carlo sampling based parameter optimization for dense optical-flow deformable image registration of 4DCT lung anatomy

    NASA Astrophysics Data System (ADS)

    Dou, Tai H.; Min, Yugang; Neylon, John; Thomas, David; Kupelian, Patrick; Santhanam, Anand P.

    2016-03-01

    Deformable image registration (DIR) is an important step in radiotherapy treatment planning. An optimal input registration parameter set is critical to achieve the best registration performance with the specific algorithm. Methods In this paper, we investigated a parameter optimization strategy for Optical-flow based DIR of the 4DCT lung anatomy. A novel fast simulated annealing with adaptive Monte Carlo sampling algorithm (FSA-AMC) was investigated for solving the complex non-convex parameter optimization problem. The metric for registration error for a given parameter set was computed using landmark-based mean target registration error (mTRE) between a given volumetric image pair. To reduce the computational time in the parameter optimization process, a GPU based 3D dense optical-flow algorithm was employed for registering the lung volumes. Numerical analyses on the parameter optimization for the DIR were performed using 4DCT datasets generated with breathing motion models and open-source 4DCT datasets. Results showed that the proposed method efficiently estimated the optimum parameters for optical-flow and closely matched the best registration parameters obtained using an exhaustive parameter search method.

  4. Probing Hypergiant Mass Loss with Adaptive Optics Imaging and Polarimetry in the Infrared: MMT-Pol and LMIRCam Observations of IRC +10420 and VY Canis Majoris

    NASA Astrophysics Data System (ADS)

    Shenoy, Dinesh P.; Jones, Terry J.; Packham, Chris; Lopez-Rodriguez, Enrique

    2015-07-01

    We present 2-5 μm adaptive optics (AO) imaging and polarimetry of the famous hypergiant stars IRC +10420 and VY Canis Majoris. The imaging polarimetry of IRC +10420 with MMT-Pol at 2.2 μ {m} resolves nebular emission with intrinsic polarization of 30%, with a high surface brightness indicating optically thick scattering. The relatively uniform distribution of this polarized emission both radially and azimuthally around the star confirms previous studies that place the scattering dust largely in the plane of the sky. Using constraints on scattered light consistent with the polarimetry at 2.2 μ {m}, extrapolation to wavelengths in the 3-5 μm band predicts a scattered light component significantly below the nebular flux that is observed in our Large Binocular Telescope/LMIRCam 3-5 μm AO imaging. Under the assumption this excess emission is thermal, we find a color temperature of ˜500 K is required, well in excess of the emissivity-modified equilibrium temperature for typical astrophysical dust. The nebular features of VY CMa are found to be highly polarized (up to 60%) at 1.3 μm, again with optically thick scattering required to reproduce the observed surface brightness. This star’s peculiar nebular feature dubbed the “Southwest Clump” is clearly detected in the 3.1 μm polarimetry as well, which, unlike IRC +10420, is consistent with scattered light alone. The high intrinsic polarizations of both hypergiants’ nebulae are compatible with optically thick scattering for typical dust around evolved dusty stars, where the depolarizing effect of multiple scatters is mitigated by the grains’ low albedos. Observations reported here were obtained at the MMT Observatory, a joint facility of the Smithsonian Institution and the University of Arizona.

  5. Probing Hypergiant Mass Loss with Adaptive Optics Imaging and Polarimetry in the Infrared: MMT-Pol and LMIRCam Observations of IRC +10420 and VY Canis Majoris

    NASA Astrophysics Data System (ADS)

    Shenoy, Dinesh P.; Jones, Terry J.; Packham, Chris; Lopez-Rodriguez, Enrique

    2015-07-01

    We present 2–5 μm adaptive optics (AO) imaging and polarimetry of the famous hypergiant stars IRC +10420 and VY Canis Majoris. The imaging polarimetry of IRC +10420 with MMT-Pol at 2.2 μ {m} resolves nebular emission with intrinsic polarization of 30%, with a high surface brightness indicating optically thick scattering. The relatively uniform distribution of this polarized emission both radially and azimuthally around the star confirms previous studies that place the scattering dust largely in the plane of the sky. Using constraints on scattered light consistent with the polarimetry at 2.2 μ {m}, extrapolation to wavelengths in the 3–5 μm band predicts a scattered light component significantly below the nebular flux that is observed in our Large Binocular Telescope/LMIRCam 3–5 μm AO imaging. Under the assumption this excess emission is thermal, we find a color temperature of ∼500 K is required, well in excess of the emissivity-modified equilibrium temperature for typical astrophysical dust. The nebular features of VY CMa are found to be highly polarized (up to 60%) at 1.3 μm, again with optically thick scattering required to reproduce the observed surface brightness. This star’s peculiar nebular feature dubbed the “Southwest Clump” is clearly detected in the 3.1 μm polarimetry as well, which, unlike IRC +10420, is consistent with scattered light alone. The high intrinsic polarizations of both hypergiants’ nebulae are compatible with optically thick scattering for typical dust around evolved dusty stars, where the depolarizing effect of multiple scatters is mitigated by the grains’ low albedos. Observations reported here were obtained at the MMT Observatory, a joint facility of the Smithsonian Institution and the University of Arizona.

  6. Adaptive optics optical coherence tomography at 1 MHz.

    PubMed

    Kocaoglu, Omer P; Turner, Timothy L; Liu, Zhuolin; Miller, Donald T

    2014-12-01

    Image acquisition speed of optical coherence tomography (OCT) remains a fundamental barrier that limits its scientific and clinical utility. Here we demonstrate a novel multi-camera adaptive optics (AO-)OCT system for ophthalmologic use that operates at 1 million A-lines/s at a wavelength of 790 nm with 5.3 μm axial resolution in retinal tissue. Central to the spectral-domain design is a novel detection channel based on four high-speed spectrometers that receive light sequentially from a 1 × 4 optical switch assembly. Absence of moving parts enables ultra-fast (50ns) and precise switching with low insertion loss (-0.18 dB per channel). This manner of control makes use of all available light in the detection channel and avoids camera dead-time, both critical for imaging at high speeds. Additional benefit in signal-to-noise accrues from the larger numerical aperture afforded by the use of AO and yields retinal images of comparable dynamic range to that of clinical OCT. We validated system performance by a series of experiments that included imaging in both model and human eyes. We demonstrated the performance of our MHz AO-OCT system to capture detailed images of individual retinal nerve fiber bundles and cone photoreceptors. This is the fastest ophthalmic OCT system we know of in the 700 to 915 nm spectral band.

  7. Robotic visible-light laser adaptive optics

    NASA Astrophysics Data System (ADS)

    Baranec, Christoph; Riddle, Reed; Law, Nicholas; Ramaprakash, A. N.; Tendulkar, Shriharsh; Bui, Khanh; Burse, Mahesh; Chordia, Pravin; Das, Hillol; Dekany, Richard; Kulkarni, Shrinivas; Punnadi, Sujit

    2013-12-01

    Robo-AO is the first autonomous laser adaptive optics system and science instrument operating on sky. With minimal human oversight, the system robotically executes large scale surveys, monitors long-term astrophysical dynamics and characterizes newly discovered transients, all at the visible diffraction limit. The adaptive optics setup time, from the end of the telescope slew to the beginning of an observation, is a mere ~50-60 s, enabling over 200 observations per night. The first of many envisioned systems has finished 58 nights of science observing at the Palomar Observatory 60-inch (1.5 m) telescope, with over 6,400 robotic observations executed thus far. The system will be augmented in late 2013 with a low-noise wide field infrared camera, which doubles as a tip-tilt sensor, to widen the spectral bandwidth of observations and increase available sky coverage while also enabling deeper visible imaging using adaptive-optics sharpened infrared tip-tilt guide sources. Techniques applicable to larger telescope systems will also be tested: the infrared camera will be used to demonstrate advanced multiple region-of-interest tip-tilt guiding methods, and a visitor instrument port will be used for evaluation of other instrumentation, e.g. single-mode and photonic fibers to feed compact spectrographs.

  8. Adaptive Optics for Industry and Medicine

    NASA Astrophysics Data System (ADS)

    Dainty, Christopher

    2008-01-01

    pt. 1. Wavefront correctors and control. Liquid crystal lenses for correction of presbyopia (Invited Paper) / Guoqiang Li and Nasser Peyghambarian. Converging and diverging liquid crystal lenses (oral paper) / Andrew X. Kirby, Philip J. W. Hands, and Gordon D. Love. Liquid lens technology for miniature imaging systems: status of the technology, performance of existing products and future trends (invited paper) / Bruno Berge. Carbon fiber reinforced polymer deformable mirrors for high energy laser applications (oral paper) / S. R. Restaino ... [et al.]. Tiny multilayer deformable mirrors (oral paper) / Tatiana Cherezova ... [et al.]. Performance analysis of piezoelectric deformable mirrors (oral paper) / Oleg Soloviev, Mikhail Loktev and Gleb Vdovin. Deformable membrane mirror with high actuator density and distributed control (oral paper) / Roger Hamelinck ... [et al.]. Characterization and closed-loop demonstration of a novel electrostatic membrane mirror using COTS membranes (oral paper) / David Dayton ... [et al.]. Electrostatic micro-deformable mirror based on polymer materials (oral paper) / Frederic Zamkotsian ... [et al.]. Recent progress in CMOS integrated MEMS A0 mirror development (oral paper) / A. Gehner ... [et al.]. Compact large-stroke piston-tip-tilt actuator and mirror (oral paper) / W. Noell ... [et al.]. MEMS deformable mirrors for high performance AO applications (oral paper) / Paul Bierden, Thomas Bifano and Steven Cornelissen. A versatile interferometric test-rig for the investigation and evaluation of ophthalmic AO systems (poster paper) / Steve Gruppetta, Jiang Jian Zhong and Luis Diaz-Santana. Woofer-tweeter adaptive optics (poster paper) / Thomas Farrell and Chris Dainty. Deformable mirrors based on transversal piezoeffect (poster paper) / Gleb Vdovin, Mikhail Loktev and Oleg Soloviev. Low-cost spatial light modulators for ophthalmic applications (poster paper) / Vincente Durán ... [et al.]. Latest MEMS DM developments and the path ahead

  9. Retinal Optical Coherence Tomography Imaging

    NASA Astrophysics Data System (ADS)

    Drexler, Wolfgang; Fujimoto, James G.

    The eye is essentially transparent, transmitting light with only minimal optical attenuation and scattering providing easy optical access to the anterior segment as well as the retina. For this reason, ophthalmic and especially retinal imaging has been not only the first but also most successful clinical application for optical coherence tomography (OCT). This chapter focuses on the development of OCT technology for retinal imaging. OCT has significantly improved the potential for early diagnosis, understanding of retinal disease pathogenesis, as well as monitoring disease progression and response to therapy. Development of ultrabroad bandwidth light sources and high-speed detection techniques has enabled significant improvements in ophthalmic OCT imaging performance, demonstrating the potential of three-dimensional, ultrahigh-resolution OCT (UHR OCT) to perform noninvasive optical biopsy of the living human retina, i.e., the in vivo visualization of microstructural, intraretinal morphology in situ approaching the resolution of conventional histopathology. Significant improvements in axial resolution and speed not only enable three-dimensional rendering of retinal volumes but also high-definition, two-dimensional tomograms, topographic thickness maps of all major intraretinal layers, as well as volumetric quantification of pathologic intraretinal changes. These advances in OCT technology have also been successfully applied in several animal models of retinal pathologies. The development of light sources emitting at alternative wavelengths, e.g., around #1,060 nm, not only enabled three-dimensional OCT imaging with enhanced choroidal visualization but also improved OCT performance in cataract patients due to reduced scattering losses in this wavelength region. Adaptive optics using deformable mirror technology, with unique high stroke to correct higher-order ocular aberrations, with specially designed optics to compensate chromatic aberration of the human eye, in

  10. Adaptive optics for peripheral vision

    NASA Astrophysics Data System (ADS)

    Rosén, R.; Lundström, L.; Unsbo, P.

    2012-07-01

    Understanding peripheral optical errors and their impact on vision is important for various applications, e.g. research on myopia development and optical correction of patients with central visual field loss. In this study, we investigated whether correction of higher order aberrations with adaptive optics (AO) improve resolution beyond what is achieved with best peripheral refractive correction. A laboratory AO system was constructed for correcting peripheral aberrations. The peripheral low contrast grating resolution acuity in the 20° nasal visual field of the right eye was evaluated for 12 subjects using three types of correction: refractive correction of sphere and cylinder, static closed loop AO correction and continuous closed loop AO correction. Running AO in continuous closed loop improved acuity compared to refractive correction for most subjects (maximum benefit 0.15 logMAR). The visual improvement from aberration correction was highly correlated with the subject's initial amount of higher order aberrations (p = 0.001, R 2 = 0.72). There was, however, no acuity improvement from static AO correction. In conclusion, correction of peripheral higher order aberrations can improve low contrast resolution, provided refractive errors are corrected and the system runs in continuous closed loop.

  11. Sodium guide star adaptive optics system for astronomical imaging in the visible and near-infrared. Revision 1

    SciTech Connect

    Gavel, D.T.; Morris, J.R.; Vernon, R.G.

    1992-08-01

    We are building an adaptive-optic telescope system that is based on the use of an artificial guide star created by laser-induced fluorescence of the sodium mesospheric layer. This paper discusses the system design for mid-visible to near-infrared compensation of a one meter telescope at Livermore and near-infrared compensation of the ten meter Keck telescope at Mauna Kea. We calculate the expected Strehl ratio and resolution for a 69 channel deformable mirror system and also for a possible 24 channel system upgrade. With the 69 actuator system we expect near diffraction limited resolution, about 0.2 arcsec, with a Strehl ratio of about 0.5 at {gamma}=0.8 {mu}m on the 1m telescope, and resolution of about 0.05 arcsec with a Strehl ratio of about 0.5 at {gamma}=2.0 {mu}m on the 10m telescope. Resolution will be limited by the performance of the tip/tilt correction loop, which uses an off-axis natural guide star as a reference. At Livermore, our design uses an existing high power (1 kW) laser source, which is expected to provide an approximately 6th magnitude artificial guide star. This strong beacon signal allows a short integration time in the wavefront sensor so that temporal changes in the atmospheric turbulence can be tracked accurately. For Mauna Kea, we explore how the system to be built for the Livermore site would perform in the infrared, assuming a 100 W laser source.

  12. Ferroelectric optical image comparator

    DOEpatents

    Butler, Michael A.; Land, Cecil E.; Martin, Stephen J.; Pfeifer, Kent B.

    1993-01-01

    A ferroelectric optical image comparator has a lead lanthanum zirconate titanate thin-film device which is constructed with a semi-transparent or transparent conductive first electrode on one side of the thin film, a conductive metal second electrode on the other side of the thin film, and the second electrode is in contact with a nonconducting substrate. A photoinduced current in the device represents the dot product between a stored image and an image projected onto the first electrode. One-dimensional autocorrelations are performed by measuring this current while displacing the projected image.

  13. Ferroelectric optical image comparator

    DOEpatents

    Butler, M.A.; Land, C.E.; Martin, S.J.; Pfeifer, K.B.

    1993-11-30

    A ferroelectric optical image comparator has a lead lanthanum zirconate titanate thin-film device which is constructed with a semi-transparent or transparent conductive first electrode on one side of the thin film, a conductive metal second electrode on the other side of the thin film, and the second electrode is in contact with a nonconducting substrate. A photoinduced current in the device represents the dot product between a stored image and an image projected onto the first electrode. One-dimensional autocorrelations are performed by measuring this current while displacing the projected image. 7 figures.

  14. Solar adaptive optics at the Observatorio del Teide, Tenerife

    NASA Astrophysics Data System (ADS)

    Soltau, Dirk; Berkefeld, Thomas; Schmidt, Dirk; von der Lühe, Oskar

    2013-10-01

    Observing the Sun with high angular resolution is difficult because the turbulence in the atmosphere is strongest during day time. In this paper we describe the principles of solar adaptive optics exemplified by the two German solar telescopes VTT and GREGOR at the Observatorio del Teide. With theses systems we obtain near diffraction limited images of the Sun. Ways to overcome the limits of conventional AO by applying multiconjugate adaptive optics (MCAO) are shown.

  15. Performance of adaptive optics at Lick Observatory

    SciTech Connect

    Olivier, S.S.; An, J.; Avicola, K.

    1994-03-01

    A prototype adaptive optics system has been developed at Lawrence Livermore National Laboratory (LLNL) for use at Lick Observatory. This system is based on an ITEX 69-actuator continuous-surface deformable mirror, a Kodak fast-framing intensified CCD camera, and a Mercury VME board containing four Intel i860 processors. The system has been tested using natural reference stars on the 40-inch Nickel telescope at Lick Observatory yielding up to a factor of 10 increase in image peak intensity and a factor of 6 reduction in image full width at half maximum (FWHM). These results are consistent with theoretical expectations. In order to improve performance, the intensified CCD camera will be replaced by a high-quantum-efficiency low-noise fast CCD camera built for LLNL by Adaptive Optics Associates using a chip developed by Lincoln Laboratory, and the 69-actuator deformable mirror will be replaced by a 127-actuator deformable mirror developed at LLNL. With these upgrades, the system should perform well in median seeing conditions on the 120-inch Shane telescope for observing wavelengths longer than {approximately}1 {mu}m and using natural reference stars brighter than m{sub R} {approximately} 10 or using the laser currently being developed at LLNL for use at Lick Observatory to generate a sodium-layer reference star.

  16. Optical imaging. Expansion microscopy.

    PubMed

    Chen, Fei; Tillberg, Paul W; Boyden, Edward S

    2015-01-30

    In optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. We discovered that by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced closer than the optical diffraction limit can be isotropically separated and optically resolved, a process we call expansion microscopy (ExM). Thus, this process can be used to perform scalable superresolution microscopy with diffraction-limited microscopes. We demonstrate ExM with apparent ~70-nanometer lateral resolution in both cultured cells and brain tissue, performing three-color superresolution imaging of ~10(7) cubic micrometers of the mouse hippocampus with a conventional confocal microscope.

  17. Laser guide stars and adaptive optics for astronomy

    SciTech Connect

    Max, C.E.

    1992-07-15

    Five papers are included: feasibility experiment for sodium-alyer laser guide stars at LLNL; system design for a high power sodium beacon laser; sodium guide star adaptive optics system for astronomical imaging in the visible and near-infrared; high frame-rate, large field wavefront sensor; and resolution limits for ground-based astronomical imaging. Figs, tabs, refs.

  18. DKIST Adaptive Optics System: Simulation Results

    NASA Astrophysics Data System (ADS)

    Marino, Jose; Schmidt, Dirk

    2016-05-01

    The 4 m class Daniel K. Inouye Solar Telescope (DKIST), currently under construction, will be equipped with an ultra high order solar adaptive optics (AO) system. The requirements and capabilities of such a solar AO system are beyond those of any other solar AO system currently in operation. We must rely on solar AO simulations to estimate and quantify its performance.We present performance estimation results of the DKIST AO system obtained with a new solar AO simulation tool. This simulation tool is a flexible and fast end-to-end solar AO simulator which produces accurate solar AO simulations while taking advantage of current multi-core computer technology. It relies on full imaging simulations of the extended field Shack-Hartmann wavefront sensor (WFS), which directly includes important secondary effects such as field dependent distortions and varying contrast of the WFS sub-aperture images.

  19. Optical Imaging in Microstructures

    SciTech Connect

    Aker, P. M.

    2001-04-11

    This research was focused on developing morphology-dependent stimulated raman scattering (MDSRS) spectroscopy as an analytic optical imaging technique. MDSRS uses the cavity modes (called morphology dependent resonances, MDRs) associated with axisymmetric dielectric microstructures to generate nonlinear optical signals. Since different cavity modes span different regions inside the microstructure, it becomes possible to generate location-specific spectra. The information gotten from MDSRS imaging experiments is analogous with that generated from magnetic resonance imaging (MRI) studies in that spatial variations in chemical composition and molecular configuration within a structure can be mapped out. The authors demonstrated that MDSRS imaging is feasible and is free from nonlinear artifact. They did this by measuring the molecular structure variations that are present in the interfaces of 180 {micro}m dia. charged water droplets. The 4 publications that resulted from these studies are attached. From a chemical perspective a water droplet is, however, a simple thing. Will it be possible to use MDSRS imaging to study more complex systems such as combusting fuel droplets, layered polymer or glass fibers, or biological cells? The long-term goal of the research was to answer this question. The answer they have come up with is yes and no. The results on nitrate aerosols show that it is possible to do imaging studies on optically non-absorbing, ion containing systems, but that the ultimate sensitivity is dictated by ion concentration. hence systems containing large quantities of mobile ions will be difficult to look at, so this essentially eliminates being able to look at biological samples in situ. But on the positive side, organic systems, such as layered polymer and glass fibers, and combusting organic fuel droplets can be looked at with MDSRS imaging.

  20. Initial concepts for CELT adaptive optics

    NASA Astrophysics Data System (ADS)

    Dekany, Richard G.; Bauman, Brian J.; Gavel, Donald T.; Troy, Mitchell; Macintosh, Bruce A.; Britton, Matthew C.

    2003-02-01

    The California Extremely Large Telescope (CELT) project has recently completed a 12-month conceptual design phase that has investigated major technology challenges in a number of Observatory subsystems, including adaptive optics (AO). The goal of this effort was not to adopt one or more specific AO architectures. Rather, it was to investigate the feasibility of adaptive optics correction of a 30-meter diameter telescope and to suggest realistic cost ceilings for various adaptive optics capabilities. We present here the key design issues uncovered during conceptual design and present two non-exclusive ‘baseline" adaptive optics concepts that are expected to be further developed during the following preliminary design phase. Further analysis, detailed engineering trade studies, and certain laboratory and telescope experiments must be performed, and key component technology prototypes demonstrated, prior to adopting one or more adaptive optics systems architectures for realization.

  1. Adaptive optics in digital micromirror based confocal microscopy

    NASA Astrophysics Data System (ADS)

    Pozzi, P.; Wilding, D.; Soloviev, O.; Vdovin, G.; Verhaegen, M.

    2016-03-01

    This proceeding reports early results in the development of a new technique for adaptive optics in confocal microscopy. The term adaptive optics refers to the branch of optics in which an active element in the optical system is used to correct inhomogeneities in the media through which light propagates. In its most classical form, mostly used in astronomical imaging, adaptive optics is achieved through a closed loop in which the actuators of a deformable mirror are driven by a wavefront sensor. This approach is severely limited in fluorescence microscopy, as the use of a wavefront sensor requires the presence of a bright, point like source in the field of view, a condition rarely satisfied in microscopy samples. Previously reported approaches to adaptive optics in fluorescence microscopy are therefore limited to the inclusion of fluorescent microspheres in the sample, to use as bright stars for wavefront sensors, or time consuming sensorless optimization procedures, requiring several seconds of optimization before the acquisition of a single image. We propose an alternative approach to the problem, implementing sensorless adaptive optics in a Programmable array microscope. A programmable array microscope is a microscope based on a digital micromirror device, in which the single elements of the micromirror act both as point sources and pinholes.

  2. Dynamic optically multiplexed imaging

    NASA Astrophysics Data System (ADS)

    Rachlin, Yaron; Shah, Vinay; Shepard, R. Hamilton; Shih, Tina

    2015-09-01

    Optically multiplexed imagers overcome the tradeoff between field of view and resolution by superimposing images from multiple fields of view onto a single focal plane. In this paper, we consider the implications of independently shifting each field of view at a rate exceeding the frame rate of the focal plane array and with a precision that can exceed the pixel pitch. A sequence of shifts enables the reconstruction of the underlying scene, with the number of frames required growing inversely with the number of multiplexed images. As a result, measurements from a sufficiently fast sampling sensor can be processed to yield a low distortion image with more pixels than the original focal plane array, a wider field of view than the original optical design, and an aspect ratio different than the original lens. This technique can also enable the collection of low-distortion, wide field of view videos. A sequence of sub-pixel spatial shifts extends this capability to allow the recovery of a wide field of view scene at sub-pixel resolution. To realize this sensor concept, a novel and compact divided aperture multiplexed sensor, capable of rapidly and precisely shifting its fields of view, was prototyped. Using this sensor, we recover twenty-four megapixel images from a four-megapixel focal plane and show the feasibility of simultaneous de-multiplexing and super-resolution.

  3. Optical Design and Optimization of Translational Reflective Adaptive Optics Ophthalmoscopes

    NASA Astrophysics Data System (ADS)

    Sulai, Yusufu N. B.

    The retina serves as the primary detector for the biological camera that is the eye. It is composed of numerous classes of neurons and support cells that work together to capture and process an image formed by the eye's optics, which is then transmitted to the brain. Loss of sight due to retinal or neuro-ophthalmic disease can prove devastating to one's quality of life, and the ability to examine the retina in vivo is invaluable in the early detection and monitoring of such diseases. Adaptive optics (AO) ophthalmoscopy is a promising diagnostic tool in early stages of development, still facing significant challenges before it can become a clinical tool. The work in this thesis is a collection of projects with the overarching goal of broadening the scope and applicability of this technology. We begin by providing an optical design approach for AO ophthalmoscopes that reduces the aberrations that degrade the performance of the AO correction. Next, we demonstrate how to further improve image resolution through the use of amplitude pupil apodization and non-common path aberration correction. This is followed by the development of a viewfinder which provides a larger field of view for retinal navigation. Finally, we conclude with the development of an innovative non-confocal light detection scheme which improves the non-invasive visualization of retinal vasculature and reveals the cone photoreceptor inner segments in healthy and diseased eyes.

  4. Adaptive atom-optics in atom interferometry

    NASA Astrophysics Data System (ADS)

    Marable, M. L.; Savard, T. A.; Thomas, J. E.

    1997-02-01

    We suggest a general technique for creating virtual atom-optical elements which are adaptive. The shape and position of these elements is determined by the frequency distribution for optical fields which induce transitions in a high gradient potential. This adaptive method is demonstrated in an all-optical atom interferometer, by creating either a variable optical slit or a variable optical grating which is scanned across the atomic spatial patterns to measure the fringes. This method renders mechanical motion of the interferometer elements unnecessary.

  5. High-Resolution Mid-Infrared Imaging of the Asymptotic Giant Branch Star RV Bootis with the Steward Observatory Adaptive Optics System

    NASA Astrophysics Data System (ADS)

    Biller, B. A.; Close, L. M.; Li, A.; Bieging, J. H.; Hoffmann, W. F.; Hinz, P. M.; Miller, D.; Brusa, G.; Lloyd-Hart, M.; Wildi, F.; Potter, D.; Oppenheimer, B. D.

    2005-02-01

    We present high-resolution (~0.1"), very high Strehl ratio (0.97+/-0.03) mid-IR adaptive optics (AO) images of the asymptotic giant branch (AGB) star RV Boo utilizing the MMT adaptive secondary AO system. RV Boo was observed at a number of wavelengths over two epochs (9.8 μm in 2003 May and 8.8, 9.8, and 11.7 μm in 2004 February) and appeared slightly extended at all wavelengths. While the extension is very slight at 8.8 and 11.7 μm, the extension is somewhat more pronounced at 9.8 μm. With such high Strehl ratios, we can achieve superresolutions of 0.1" by deconvolving RV Boo with a point-spread function (PSF) derived from an unresolved star. We tentatively resolve RV Boo into a 0.16" FWHM extension at a position angle of 120°. At a distance of 390+250-100 pc, this corresponds to a FWHM of 60+40-15 AU. We measure a total flux at 9.8 μm of 145+/-24 Jy for the disk and star. Based on a dust thermal emission model for the observed IR spectral energy distribution and the 9.8 μm AO image, we derive a disk dust mass of 1.6×10-6 Msolar and an inclination of 30°-45° from edge-on. We discuss whether the dust disk observed around RV Boo is an example of the early stages in the formation of asymmetric structure in planetary nebulae.

  6. AIDA: An Adaptive Image Deconvolution Algorithm

    NASA Astrophysics Data System (ADS)

    Hom, Erik; Marchis, F.; Lee, T. K.; Haase, S.; Agard, D. A.; Sedat, J. W.

    2007-10-01

    We recently described an adaptive image deconvolution algorithm (AIDA) for myopic deconvolution of multi-frame and three-dimensional data acquired through astronomical and microscopic imaging [Hom et al., J. Opt. Soc. Am. A 24, 1580 (2007)]. AIDA is a reimplementation and extension of the MISTRAL method developed by Mugnier and co-workers and shown to yield object reconstructions with excellent edge preservation and photometric precision [J. Opt. Soc. Am. A 21, 1841 (2004)]. Written in Numerical Python with calls to a robust constrained conjugate gradient method, AIDA has significantly improved run times over the original MISTRAL implementation. AIDA includes a scheme to automatically balance maximum-likelihood estimation and object regularization, which significantly decreases the amount of time and effort needed to generate satisfactory reconstructions. Here, we present a gallery of results demonstrating the effectiveness of AIDA in processing planetary science images acquired using adaptive-optics systems. Offered as an open-source alternative to MISTRAL, AIDA is available for download and further development at: http://msg.ucsf.edu/AIDA. This work was supported in part by the W. M. Keck Observatory, the National Institutes of Health, NASA, the National Science Foundation Science and Technology Center for Adaptive Optics at UC-Santa Cruz, and the Howard Hughes Medical Institute.

  7. The Coming of Age of Adaptive Optics

    NASA Astrophysics Data System (ADS)

    1995-10-01

    How Ground-Based Astronomers Beat the Atmosphere Adaptive Optics (AO) is the new ``wonder-weapon'' in ground-based astronomy. By means of advanced electro-optical devices at their telescopes, astronomers are now able to ``neutralize'' the image-smearing turbulence of the terrestrial atmosphere (seen by the unaided eye as the twinkling of stars) so that much sharper images can be obtained than before. In practice, this is done with computer-controlled, flexible mirrors which refocus the blurred images up to 100 times per second, i.e. at a rate that is faster than the changes in the atmospheric turbulence. This means that finer details in astronomical objects can be studied and also - because of the improved concentration of light in the telescope's focal plane - that fainter objects can be observed. At the moment, Adaptive Optics work best in the infrared part of spectrum, but at some later time it may also significantly improve observations at the shorter wavelengths of visible light. The many-sided aspects of this new technology and its impact on astronomical instrumentation was the subject of a recent AO conference [1] with over 150 participants from about 30 countries, presenting a total of more than 100 papers. The Introduction of AO Techniques into Astronomy The scope of this meeting was the design, fabrication and testing of AO systems, characterisation of the sources of atmospheric disturbance, modelling of compensation systems, individual components, astronomical AO results, non-astronomical applications, laser guide star systems, non-linear optical phase conjugation, performance evaluation, and other areas of this wide and complex field, in which front-line science and high technology come together in a new and powerful symbiosis. One of the specific goals of the meeting was to develop contacts between AO scientists and engineers in the western world and their colleagues in Russia and Asia. For the first time at a conference of this type, nine Russian

  8. Optical Design for Extremely Large Telescope Adaptive Optics Systems

    SciTech Connect

    Bauman, B J

    2003-11-26

    Designing an adaptive optics (AO) system for extremely large telescopes (ELT's) will present new optical engineering challenges. Several of these challenges are addressed in this work, including first-order design of multi-conjugate adaptive optics (MCAO) systems, pyramid wavefront sensors (PWFS's), and laser guide star (LGS) spot elongation. MCAO systems need to be designed in consideration of various constraints, including deformable mirror size and correction height. The y,{bar y} method of first-order optical design is a graphical technique that uses a plot with marginal and chief ray heights as coordinates; the optical system is represented as a segmented line. This method is shown to be a powerful tool in designing MCAO systems. From these analyses, important conclusions about configurations are derived. PWFS's, which offer an alternative to Shack-Hartmann (SH) wavefront sensors (WFS's), are envisioned as the workhorse of layer-oriented adaptive optics. Current approaches use a 4-faceted glass pyramid to create a WFS analogous to a quad-cell SH WFS. PWFS's and SH WFS's are compared and some newly-considered similarities and PWFS advantages are presented. Techniques to extend PWFS's are offered: First, PWFS's can be extended to more pixels in the image by tiling pyramids contiguously. Second, pyramids, which are difficult to manufacture, can be replaced by less expensive lenslet arrays. An approach is outlined to convert existing SH WFS's to PWFS's for easy evaluation of PWFS's. Also, a demonstration of PWFS's in sensing varying amounts of an aberration is presented. For ELT's, the finite altitude and finite thickness of LGS's means that the LGS will appear elongated from the viewpoint of subapertures not directly under the telescope. Two techniques for dealing with LGS spot elongation in SH WFS's are presented. One method assumes that the laser will be pulsed and uses a segmented micro-electromechanical system (MEMS) to track the LGS light subaperture by

  9. Instrumentation in Diffuse Optical Imaging

    PubMed Central

    Zhang, Xiaofeng

    2014-01-01

    Diffuse optical imaging is highly versatile and has a very broad range of applications in biology and medicine. It covers diffuse optical tomography, fluorescence diffuse optical tomography, bioluminescence, and a number of other new imaging methods. These methods of diffuse optical imaging have diversified instrument configurations but share the same core physical principle – light propagation in highly diffusive media, i.e., the biological tissue. In this review, the author summarizes the latest development in instrumentation and methodology available to diffuse optical imaging in terms of system architecture, light source, photo-detection, spectral separation, signal modulation, and lastly imaging contrast. PMID:24860804

  10. Constraints on Extrasolar Planet Populations from VLT NACO/SDI and MMT SDI and Direct Adaptive Optics Imaging Surveys: Giant Planets are Rare at Large Separations

    NASA Astrophysics Data System (ADS)

    Nielsen, Eric L.; Close, Laird M.; Biller, Beth A.; Masciadri, Elena; Lenzen, Rainer

    2008-02-01

    We examine the implications for the distribution of extrasolar planets based on the null results from two of the largest direct imaging surveys published to date. Combining the measured contrast curves from 22 of the stars observed with the VLT NACO adaptive optics system by Masciadri and coworkers and 48 of the stars observed with the VLT NACO SDI and MMT SDI devices by Biller and coworkers (for a total of 60 unique stars), we consider what distributions of planet masses and semimajor axes can be ruled out by these data, based on Monte Carlo simulations of planet populations. We can set the following upper limit with 95% confidence: the fraction of stars with planets with semimajor axis between 20 and 100 AU, and mass above 4 MJup, is 20% or less. Also, with a distribution of planet mass of dN/dM propto M-1.16 in the range of 0.5-13 MJup, we can rule out a power-law distribution for semimajor axis (dN/da propto aα) with index 0 and upper cutoff of 18 AU, and index -0.5 with an upper cutoff of 48 AU. For the distribution suggested by Cumming et al., a power-law of index -0.61, we can place an upper limit of 75 AU on the semimajor axis distribution. In general, we find that even null results from direct imaging surveys are very powerful in constraining the distributions of giant planets (0.5-13 MJup) at large separations, but more work needs to be done to close the gap between planets that can be detected by direct imaging, and those to which the radial velocity method is sensitive.

  11. Investigation of Adaptive Optics Imaging Biomarkers for Detecting Pathological Changes of the Cone Mosaic in Patients with Type 1 Diabetes Mellitus

    PubMed Central

    Lombardo, Marco; Parravano, Mariacristina; Serrao, Sebastiano; Ziccardi, Lucia; Giannini, Daniela; Lombardo, Giuseppe

    2016-01-01

    Purpose To investigate a set of adaptive optics (AO) imaging biomarkers for the assessment of changes of the cone mosaic spatial arrangement in patients with type 1 diabetes mellitus (DM1). Methods 16 patients with ≥20/20 visual acuity and a diagnosis of DM1 in the past 8 years to 37 years and 20 age-matched healthy volunteers were recruited in this study. Cone density, cone spacing and Voronoi diagrams were calculated on 160x160 μm images of the cone mosaic acquired with an AO flood illumination retinal camera at 1.5 degrees eccentricity from the fovea along all retinal meridians. From the cone spacing measures and Voronoi diagrams, the linear dispersion index (LDi) and the heterogeneity packing index (HPi) were computed respectively. Logistic regression analysis was conducted to discriminate DM1 patients without diabetic retinopathy from controls using the cone metrics as predictors. Results Of the 16 DM1 patients, eight had no signs of diabetic retinopathy (noDR) and eight had mild nonproliferative diabetic retinopathy (NPDR) on fundoscopy. On average, cone density, LDi and HPi values were significantly different (P<0.05) between noDR or NPDR eyes and controls, with these differences increasing with duration of diabetes. However, each cone metric alone was not sufficiently sensitive to discriminate entirely between membership of noDR cases and controls. The complementary use of all the three cone metrics in the logistic regression model gained 100% accuracy to identify noDR cases with respect to controls. Conclusion The present set of AO imaging biomarkers identified reliably abnormalities in the spatial arrangement of the parafoveal cones in DM1 patients, even when no signs of diabetic retinopathy were seen on fundoscopy. PMID:26963392

  12. Methods for investigating the local spatial anisotropy and the preferred orientation of cones in adaptive optics retinal images.

    PubMed

    Cooper, Robert F; Lombardo, Marco; Carroll, Joseph; Sloan, Kenneth R; Lombardo, Giuseppe

    2016-01-01

    The ability to noninvasively image the cone photoreceptor mosaic holds significant potential as a diagnostic for retinal disease. Central to the realization of this potential is the development of sensitive metrics for characterizing the organization of the mosaic. Here we evaluated previously-described and newly-developed (Fourier- and Radon-based) methods of measuring cone orientation in simulated and real images of the parafoveal cone mosaic. The proposed algorithms correlated well across both simulated and real mosaics, suggesting that each algorithm provides an accurate description of photoreceptor orientation. Despite high agreement between algorithms, each performed differently in response to image intensity variation and cone coordinate jitter. The integration property of the Fourier transform allowed the Fourier-based method to be resistant to cone coordinate jitter and perform the most robustly of all three algorithms. Conversely, when there is good image quality but unreliable cone identification, the Radon algorithm performed best. Finally, in cases where the cone coordinate reliability was excellent, the method previously described by Pum and colleagues performed best. These descriptors are complementary to conventional descriptive metrics of the cone mosaic, such as cell density and spacing, and have the potential to aid in the detection of photoreceptor pathology. PMID:27484961

  13. On the possibility of intraocular adaptive optics

    NASA Astrophysics Data System (ADS)

    Vdovin, Gleb; Loktev, Mikhail; Naumov, Alexander

    2003-04-01

    We consider the technical possibility of an adaptive contact lens and an adaptive eye lens implant based on the modal liquid crystal wavefront corrector, aimed to correct the accommodation loss and higher-order aberrations of the human eye. Our first demonstrator with 5 mm optical aperture is capable of changing the focusing power in the range of 0 to +3 diopters and can be controlled via a wireless capacitive link. These properties make the corrector potentially suitable for implantation into the human eye or for use as an adaptive contact lens. We also discuss possible feedback strategies, aimed to improve visual acuity and to achieve supernormal vision with implantable adaptive optics.

  14. Object-oriented Matlab adaptive optics toolbox

    NASA Astrophysics Data System (ADS)

    Conan, R.; Correia, C.

    2014-08-01

    Object-Oriented Matlab Adaptive Optics (OOMAO) is a Matlab toolbox dedicated to Adaptive Optics (AO) systems. OOMAO is based on a small set of classes representing the source, atmosphere, telescope, wavefront sensor, Deformable Mirror (DM) and an imager of an AO system. This simple set of classes allows simulating Natural Guide Star (NGS) and Laser Guide Star (LGS) Single Conjugate AO (SCAO) and tomography AO systems on telescopes up to the size of the Extremely Large Telescopes (ELT). The discrete phase screens that make the atmosphere model can be of infinite size, useful for modeling system performance on large time scales. OOMAO comes with its own parametric influence function model to emulate different types of DMs. The cone effect, altitude thickness and intensity profile of LGSs are also reproduced. Both modal and zonal modeling approach are implemented. OOMAO has also an extensive library of theoretical expressions to evaluate the statistical properties of turbulence wavefronts. The main design characteristics of the OOMAO toolbox are object-oriented modularity, vectorized code and transparent parallel computing. OOMAO has been used to simulate and to design the Multi-Object AO prototype Raven at the Subaru telescope and the Laser Tomography AO system of the Giant Magellan Telescope. In this paper, a Laser Tomography AO system on an ELT is simulated with OOMAO. In the first part, we set-up the class parameters and we link the instantiated objects to create the source optical path. Then we build the tomographic reconstructor and write the script for the pseudo-open-loop controller.

  15. TESTING THE APODIZED PUPIL LYOT CORONAGRAPH ON THE LABORATORY FOR ADAPTIVE OPTICS EXTREME ADAPTIVE OPTICS TESTBED

    SciTech Connect

    Thomas, Sandrine J.; Dillon, Daren; Gavel, Donald; Macintosh, Bruce; Sivaramakrishnan, Anand E-mail: dillon@ucolick.org E-mail: soummer@stsci.edu E-mail: anand@amnh.org

    2011-10-15

    We present testbed results of the Apodized Pupil Lyot Coronagraph (APLC) at the Laboratory for Adaptive Optics (LAO). These results are part of the validation and tests of the coronagraph and of the Extreme Adaptive Optics (ExAO) for the Gemini Planet Imager (GPI). The apodizer component is manufactured with a halftone technique using black chrome microdots on glass. Testing this APLC (like any other coronagraph) requires extremely good wavefront correction, which is obtained to the 1 nm rms level using the microelectricalmechanical systems (MEMS) technology, on the ExAO visible testbed of the LAO at the University of Santa Cruz. We used an APLC coronagraph without central obstruction, both with a reference super-polished flat mirror and with the MEMS to obtain one of the first images of a dark zone in a coronagraphic image with classical adaptive optics using a MEMS deformable mirror (without involving dark hole algorithms). This was done as a complementary test to the GPI coronagraph testbed at American Museum of Natural History, which studied the coronagraph itself without wavefront correction. Because we needed a full aperture, the coronagraph design is very different from the GPI design. We also tested a coronagraph with central obstruction similar to that of GPI. We investigated the performance of the APLC coronagraph and more particularly the effect of the apodizer profile accuracy on the contrast. Finally, we compared the resulting contrast to predictions made with a wavefront propagation model of the testbed to understand the effects of phase and amplitude errors on the final contrast.

  16. Testing the Apodized Pupil Lyot Coronagraph on the Laboratory for Adaptive Optics Extreme Adaptive Optics Testbed

    NASA Astrophysics Data System (ADS)

    Thomas, Sandrine J.; Soummer, Rémi; Dillon, Daren; Macintosh, Bruce; Gavel, Donald; Sivaramakrishnan, Anand

    2011-10-01

    We present testbed results of the Apodized Pupil Lyot Coronagraph (APLC) at the Laboratory for Adaptive Optics (LAO). These results are part of the validation and tests of the coronagraph and of the Extreme Adaptive Optics (ExAO) for the Gemini Planet Imager (GPI). The apodizer component is manufactured with a halftone technique using black chrome microdots on glass. Testing this APLC (like any other coronagraph) requires extremely good wavefront correction, which is obtained to the 1 nm rms level using the microelectricalmechanical systems (MEMS) technology, on the ExAO visible testbed of the LAO at the University of Santa Cruz. We used an APLC coronagraph without central obstruction, both with a reference super-polished flat mirror and with the MEMS to obtain one of the first images of a dark zone in a coronagraphic image with classical adaptive optics using a MEMS deformable mirror (without involving dark hole algorithms). This was done as a complementary test to the GPI coronagraph testbed at American Museum of Natural History, which studied the coronagraph itself without wavefront correction. Because we needed a full aperture, the coronagraph design is very different from the GPI design. We also tested a coronagraph with central obstruction similar to that of GPI. We investigated the performance of the APLC coronagraph and more particularly the effect of the apodizer profile accuracy on the contrast. Finally, we compared the resulting contrast to predictions made with a wavefront propagation model of the testbed to understand the effects of phase and amplitude errors on the final contrast.

  17. Adaptive optical interconnects: the ADDAPT project

    NASA Astrophysics Data System (ADS)

    Henker, Ronny; Pliva, Jan; Khafaji, Mahdi; Ellinger, Frank; Toifl, Thomas; Offrein, Bert; Cevrero, Alessandro; Oezkaya, Ilter; Seifried, Marc; Ledentsov, Nikolay; Kropp, Joerg-R.; Shchukin, Vitaly; Zoldak, Martin; Halmo, Leos; Turkiewicz, Jaroslaw; Meredith, Wyn; Eddie, Iain; Georgiades, Michael; Charalambides, Savvas; Duis, Jeroen; van Leeuwen, Pieter

    2015-09-01

    Existing optical networks are driven by dynamic user and application demands but operate statically at their maximum performance. Thus, optical links do not offer much adaptability and are not very energy-efficient. In this paper a novel approach of implementing performance and power adaptivity from system down to optical device, electrical circuit and transistor level is proposed. Depending on the actual data load, the number of activated link paths and individual device parameters like bandwidth, clock rate, modulation format and gain are adapted to enable lowering the components supply power. This enables flexible energy-efficient optical transmission links which pave the way for massive reductions of CO2 emission and operating costs in data center and high performance computing applications. Within the FP7 research project Adaptive Data and Power Aware Transceivers for Optical Communications (ADDAPT) dynamic high-speed energy-efficient transceiver subsystems are developed for short-range optical interconnects taking up new adaptive technologies and methods. The research of eight partners from industry, research and education spanning seven European countries includes the investigation of several adaptive control types and algorithms, the development of a full transceiver system, the design and fabrication of optical components and integrated circuits as well as the development of high-speed, low loss packaging solutions. This paper describes and discusses the idea of ADDAPT and provides an overview about the latest research results in this field.

  18. Deconvolution of post-adaptive optics images of faint circumstellar environments by means of the inexact Bregman procedure

    NASA Astrophysics Data System (ADS)

    Benfenati, A.; La Camera, A.; Carbillet, M.

    2016-02-01

    Aims: High-dynamic range images of astrophysical objects present some difficulties in their restoration because of the presence of very bright point-wise sources surrounded by faint and smooth structures. We propose a method that enables the restoration of this kind of images by taking these kinds of sources into account and, at the same time, improving the contrast enhancement in the final image. Moreover, the proposed approach can help to detect the position of the bright sources. Methods: The classical variational scheme in the presence of Poisson noise aims to find the minimum of a functional compound of the generalized Kullback-Leibler function and a regularization functional: the latter function is employed to preserve some characteristic in the restored image. The inexact Bregman procedure substitutes the regularization function with its inexact Bregman distance. This proposed scheme allows us to take under control the level of inexactness arising in the computed solution and permits us to employ an overestimation of the regularization parameter (which balances the trade-off between the Kullback-Leibler and the Bregman distance). This aspect is fundamental, since the estimation of this kind of parameter is very difficult in the presence of Poisson noise. Results: The inexact Bregman procedure is tested on a bright unresolved binary star with a faint circumstellar environment. When the sources' position is exactly known, this scheme provides us with very satisfactory results. In case of inexact knowledge of the sources' position, it can in addition give some useful information on the true positions. Finally, the inexact Bregman scheme can be also used when information about the binary star's position concerns a connected region instead of isolated pixels.

  19. Amplitude variations on the Extreme Adaptive Optics testbed

    SciTech Connect

    Evans, J; Thomas, S; Dillon, D; Gavel, D; Phillion, D; Macintosh, B

    2007-08-14

    High-contrast adaptive optics systems, such as those needed to image extrasolar planets, are known to require excellent wavefront control and diffraction suppression. At the Laboratory for Adaptive Optics on the Extreme Adaptive Optics testbed, we have already demonstrated wavefront control of better than 1 nm rms within controllable spatial frequencies. Corresponding contrast measurements, however, are limited by amplitude variations, including those introduced by the micro-electrical-mechanical-systems (MEMS) deformable mirror. Results from experimental measurements and wave optic simulations of amplitude variations on the ExAO testbed are presented. We find systematic intensity variations of about 2% rms, and intensity variations with the MEMS to be 6%. Some errors are introduced by phase and amplitude mixing because the MEMS is not conjugate to the pupil, but independent measurements of MEMS reflectivity suggest that some error is introduced by small non-uniformities in the reflectivity.

  20. Wide field strip-imaging optical system

    NASA Technical Reports Server (NTRS)

    Vaughan, Arthur H. (Inventor)

    1994-01-01

    A strip imaging wide angle optical system is provided. The optical system is provided with a 'virtual' material stop to avoid aberrational effects inherent in wide angle optical systems. The optical system includes a spherical mirror section for receiving light from a 180-degree strip or arc of a target image. Light received by the spherical mirror section is reflected to a frusto-conical mirror section for subsequent rereflection to a row of optical fibers. Each optical fiber transmits a portion of the received light to a detector. The optical system exploits the narrow cone of acceptance associated with optical fibers to substantially eliminate vignetting effects inherent in wide-angle systems. Further, the optical system exploits the narrow cone of acceptance of the optical fibers to substantially limit spherical aberration. The optical system is ideally suited for any application wherein a 180-degree strip image need be detected, and is particularly well adapted for use in hostile environments such as in planetary exploration.

  1. Holographic fluorescence microscopy with incoherent digital holographic adaptive optics.

    PubMed

    Jang, Changwon; Kim, Jonghyun; Clark, David C; Lee, Seungjae; Lee, Byoungho; Kim, Myung K

    2015-01-01

    Introduction of adaptive optics technology into astronomy and ophthalmology has made great contributions in these fields, allowing one to recover images blurred by atmospheric turbulence or aberrations of the eye. Similar adaptive optics improvement in microscopic imaging is also of interest to researchers using various techniques. Current technology of adaptive optics typically contains three key elements: a wavefront sensor, wavefront corrector, and controller. These hardware elements tend to be bulky, expensive, and limited in resolution, involving, for example, lenslet arrays for sensing or multiactuator deformable mirrors for correcting. We have previously introduced an alternate approach based on unique capabilities of digital holography, namely direct access to the phase profile of an optical field and the ability to numerically manipulate the phase profile. We have also demonstrated that direct access and compensation of the phase profile are possible not only with conventional coherent digital holography, but also with a new type of digital holography using incoherent light: selfinterference incoherent digital holography (SIDH). The SIDH generates a complex—i.e., amplitude plus phase—hologram from one or several interferograms acquired with incoherent light, such as LEDs, lamps, sunlight, or fluorescence. The complex point spread function can be measured using guide star illumination and it allows deterministic deconvolution of the full-field image. We present experimental demonstration of aberration compensation in holographic fluorescence microscopy using SIDH. Adaptive optics by SIDH provides new tools for improved cellular fluorescence microscopy through intact tissue layers or other types of aberrant media.

  2. Holographic fluorescence microscopy with incoherent digital holographic adaptive optics.

    PubMed

    Jang, Changwon; Kim, Jonghyun; Clark, David C; Lee, Seungjae; Lee, Byoungho; Kim, Myung K

    2015-01-01

    Introduction of adaptive optics technology into astronomy and ophthalmology has made great contributions in these fields, allowing one to recover images blurred by atmospheric turbulence or aberrations of the eye. Similar adaptive optics improvement in microscopic imaging is also of interest to researchers using various techniques. Current technology of adaptive optics typically contains three key elements: a wavefront sensor, wavefront corrector, and controller. These hardware elements tend to be bulky, expensive, and limited in resolution, involving, for example, lenslet arrays for sensing or multiactuator deformable mirrors for correcting. We have previously introduced an alternate approach based on unique capabilities of digital holography, namely direct access to the phase profile of an optical field and the ability to numerically manipulate the phase profile. We have also demonstrated that direct access and compensation of the phase profile are possible not only with conventional coherent digital holography, but also with a new type of digital holography using incoherent light: selfinterference incoherent digital holography (SIDH). The SIDH generates a complex—i.e., amplitude plus phase—hologram from one or several interferograms acquired with incoherent light, such as LEDs, lamps, sunlight, or fluorescence. The complex point spread function can be measured using guide star illumination and it allows deterministic deconvolution of the full-field image. We present experimental demonstration of aberration compensation in holographic fluorescence microscopy using SIDH. Adaptive optics by SIDH provides new tools for improved cellular fluorescence microscopy through intact tissue layers or other types of aberrant media. PMID:26146767

  3. Holographic fluorescence microscopy with incoherent digital holographic adaptive optics

    NASA Astrophysics Data System (ADS)

    Jang, Changwon; Kim, Jonghyun; Clark, David C.; Lee, Seungjae; Lee, Byoungho; Kim, Myung K.

    2015-11-01

    Introduction of adaptive optics technology into astronomy and ophthalmology has made great contributions in these fields, allowing one to recover images blurred by atmospheric turbulence or aberrations of the eye. Similar adaptive optics improvement in microscopic imaging is also of interest to researchers using various techniques. Current technology of adaptive optics typically contains three key elements: a wavefront sensor, wavefront corrector, and controller. These hardware elements tend to be bulky, expensive, and limited in resolution, involving, for example, lenslet arrays for sensing or multiactuator deformable mirrors for correcting. We have previously introduced an alternate approach based on unique capabilities of digital holography, namely direct access to the phase profile of an optical field and the ability to numerically manipulate the phase profile. We have also demonstrated that direct access and compensation of the phase profile are possible not only with conventional coherent digital holography, but also with a new type of digital holography using incoherent light: self­interference incoherent digital holography (SIDH). The SIDH generates a complex-i.e., amplitude plus phase-hologram from one or several interferograms acquired with incoherent light, such as LEDs, lamps, sunlight, or fluorescence. The complex point spread function can be measured using guide star illumination and it allows deterministic deconvolution of the full-field image. We present experimental demonstration of aberration compensation in holographic fluorescence microscopy using SIDH. Adaptive optics by SIDH provides new tools for improved cellular fluorescence microscopy through intact tissue layers or other types of aberrant media.

  4. Adaptive compensation for an optical tracking telescope

    NASA Technical Reports Server (NTRS)

    Gilbart, J. W.; Winston, G. C.

    1974-01-01

    The application of model referenced adaptive control theory to an optical tracking telescope is discussed. The capability of the adaptive technique to compensate for mount irregularities such as inertial variations and bearing friction is demonstrated via field test results on a large tracking telescope. Results are presented which show a 6 to 1 improvement in tracking accuracy for a worst-case satellite trajectory.

  5. Small scale adaptive optics experiment systems engineering

    NASA Technical Reports Server (NTRS)

    Boykin, William H.

    1993-01-01

    Assessment of the current technology relating to the laser power beaming system which in full scale is called the Beam Transmission Optical System (BTOS). Evaluation of system integration efforts are being conducted by the various government agencies and industry. Concepts are being developed for prototypes of adaptive optics for a BTOS.

  6. Optical Profilometers Using Adaptive Signal Processing

    NASA Technical Reports Server (NTRS)

    Hall, Gregory A.; Youngquist, Robert; Mikhael, Wasfy

    2006-01-01

    A method of adaptive signal processing has been proposed as the basis of a new generation of interferometric optical profilometers for measuring surfaces. The proposed profilometers would be portable, hand-held units. Sizes could be thus reduced because the adaptive-signal-processing method would make it possible to substitute lower-power coherent light sources (e.g., laser diodes) for white light sources and would eliminate the need for most of the optical components of current white-light profilometers. The adaptive-signal-processing method would make it possible to attain scanning ranges of the order of decimeters in the proposed profilometers.

  7. Optical image encryption based on diffractive imaging.

    PubMed

    Chen, Wen; Chen, Xudong; Sheppard, Colin J R

    2010-11-15

    In this Letter, we propose a method for optical image encryption based on diffractive imaging. An optical multiple random phase mask encoding system is applied, and one of the phase-only masks is selected and laterally translated along a preset direction during the encryption process. For image decryption, a phase retrieval algorithm is proposed to extract a high-quality plaintext. The feasibility and effectiveness of the proposed method are demonstrated by numerical results. The proposed method can provide a new strategy instead of conventional interference methods, and it may open up a new research perspective for optical image encryption.

  8. Adaptive Optics and NICMOS Uniqueness Space

    SciTech Connect

    Max, C.

    1999-03-22

    As part of the HST Second Decade Study a subgroup consisting of Claire Max, James Beletic, Donald McCarthy, and Keith Noll has analyzed the expected performance of near-infra-red adaptive optics systems on the new generation of 8-10 meter ground-based telescopes, for comparison with HST. In addition the subgroup has polled the adaptive optics community regarding expected adaptive optics performance over the coming five years. Responses have been received from representatives of most of the major telescopes: Gemini, VLT, Keck, LBT, and the MMT, as well as of several operational 3-4 meter telescope AO systems. The present document outlines the conclusions to date, with emphasis on aspects relevant to the NICMOS cryocooler Independent Science Review. In general the near-infra-red capabilities of the new ground-based adaptive optics systems will be complementary to the capabilities of NICMOS. For example NICMOS will have greater H-band sensitivity, broader wavelength coverage, and higher point-spread-function stability, whereas ground-based adaptive optics instruments will have higher spatial and spectral resolution. Section 2 of this report outlines the operational constraints faced by the first generation of adaptive optics (AO) systems on new 8-10 meter telescopes. Section 3 describes the areas of relative strength of near-infra-red observing from the ground via adaptive optics, compared with NICMOS. A Table gives an overview of the main strengths and weaknesses of these current-generation systems. Section 4 gives an indication of ground-based capabilities anticipated in the near future and in five to ten years. Section 5 contains a summary and conclusions.

  9. Smart adaptive optic systems using spatial light modulators.

    PubMed

    Clark, N; Banish, M; Ranganath, H S

    1999-01-01

    Many factors contribute to the aberrations induced in an optical system. Atmospheric turbulence between the object and the imaging system, physical or thermal perturbations in optical elements degrade the system's point spread function, and misaligned optics are the primary sources of aberrations that affect image quality. The design of a nonconventional real-time adaptive optic system using a micro-mirror device for wavefront correction is presented. The unconventional compensated imaging system presented offers advantages in speed, cost, power consumption, and weight. A pulsed-coupled neural network is used to as a preprocessor to enhance the performance of the wavefront sensor for low-light applications. Modeling results that characterize the system performance are presented. PMID:18252558

  10. Wavelet methods in multi-conjugate adaptive optics

    NASA Astrophysics Data System (ADS)

    Helin, T.; Yudytskiy, M.

    2013-08-01

    The next generation ground-based telescopes rely heavily on adaptive optics for overcoming the limitation of atmospheric turbulence. In the future adaptive optics modalities, like multi-conjugate adaptive optics (MCAO), atmospheric tomography is the major mathematical and computational challenge. In this severely ill-posed problem, a fast and stable reconstruction algorithm is needed that can take into account many real-life phenomena of telescope imaging. We introduce a novel reconstruction method for the atmospheric tomography problem and demonstrate its performance and flexibility in the context of MCAO. Our method is based on using locality properties of compactly supported wavelets, both in the spatial and frequency domains. The reconstruction in the atmospheric tomography problem is obtained by solving the Bayesian MAP estimator with a conjugate-gradient-based algorithm. An accelerated algorithm with preconditioning is also introduced. Numerical performance is demonstrated on the official end-to-end simulation tool OCTOPUS of European Southern Observatory.

  11. Adaptive optics assisted Fourier domain OCT with balanced detection

    NASA Astrophysics Data System (ADS)

    Meadway, A.; Bradu, A.; Hathaway, M.; Van der Jeught, S.; Rosen, R. B.; Podoleanu, A. Gh.

    2011-03-01

    Two factors are of importance to optical coherence tomography (OCT), resolution and sensitivity. Adaptive optics improves the resolution of a system by correcting for aberrations causing distortions in the wave-front. Balanced detection has been used in time domain OCT systems by removing excess photon noise, however it has not been used in Fourier domain systems, as the cameras used in the spectrometers saturated before excess photon noise becomes a problem. Advances in camera technology mean that this is no longer the case and balanced detection can now be used to improve the signal to noise ratio in a Fourier domain (FD) OCT system. An FD-OCT system, enhanced with adaptive optics, is presented and is used to show the improvement that balanced detection can provide. The signal to noise ratios of single camera detection and balanced detection are assessed and in-vivo retinal images are acquired to demonstrate better image quality when using balance detection.

  12. Lens-based wavefront sensorless adaptive optics swept source OCT

    PubMed Central

    Jian, Yifan; Lee, Sujin; Ju, Myeong Jin; Heisler, Morgan; Ding, Weiguang; Zawadzki, Robert J.; Bonora, Stefano; Sarunic, Marinko V.

    2016-01-01

    Optical coherence tomography (OCT) has revolutionized modern ophthalmology, providing depth resolved images of the retinal layers in a system that is suited to a clinical environment. Although the axial resolution of OCT system, which is a function of the light source bandwidth, is sufficient to resolve retinal features at a micrometer scale, the lateral resolution is dependent on the delivery optics and is limited by ocular aberrations. Through the combination of wavefront sensorless adaptive optics and the use of dual deformable transmissive optical elements, we present a compact lens-based OCT system at an imaging wavelength of 1060 nm for high resolution retinal imaging. We utilized a commercially available variable focal length lens to correct for a wide range of defocus commonly found in patient’s eyes, and a novel multi-actuator adaptive lens for aberration correction to achieve near diffraction limited imaging performance at the retina. With a parallel processing computational platform, high resolution cross-sectional and en face retinal image acquisition and display was performed in real time. In order to demonstrate the system functionality and clinical utility, we present images of the photoreceptor cone mosaic and other retinal layers acquired in vivo from research subjects. PMID:27278853

  13. Lens-based wavefront sensorless adaptive optics swept source OCT.

    PubMed

    Jian, Yifan; Lee, Sujin; Ju, Myeong Jin; Heisler, Morgan; Ding, Weiguang; Zawadzki, Robert J; Bonora, Stefano; Sarunic, Marinko V

    2016-01-01

    Optical coherence tomography (OCT) has revolutionized modern ophthalmology, providing depth resolved images of the retinal layers in a system that is suited to a clinical environment. Although the axial resolution of OCT system, which is a function of the light source bandwidth, is sufficient to resolve retinal features at a micrometer scale, the lateral resolution is dependent on the delivery optics and is limited by ocular aberrations. Through the combination of wavefront sensorless adaptive optics and the use of dual deformable transmissive optical elements, we present a compact lens-based OCT system at an imaging wavelength of 1060 nm for high resolution retinal imaging. We utilized a commercially available variable focal length lens to correct for a wide range of defocus commonly found in patient's eyes, and a novel multi-actuator adaptive lens for aberration correction to achieve near diffraction limited imaging performance at the retina. With a parallel processing computational platform, high resolution cross-sectional and en face retinal image acquisition and display was performed in real time. In order to demonstrate the system functionality and clinical utility, we present images of the photoreceptor cone mosaic and other retinal layers acquired in vivo from research subjects.

  14. Lens-based wavefront sensorless adaptive optics swept source OCT

    NASA Astrophysics Data System (ADS)

    Jian, Yifan; Lee, Sujin; Ju, Myeong Jin; Heisler, Morgan; Ding, Weiguang; Zawadzki, Robert J.; Bonora, Stefano; Sarunic, Marinko V.

    2016-06-01

    Optical coherence tomography (OCT) has revolutionized modern ophthalmology, providing depth resolved images of the retinal layers in a system that is suited to a clinical environment. Although the axial resolution of OCT system, which is a function of the light source bandwidth, is sufficient to resolve retinal features at a micrometer scale, the lateral resolution is dependent on the delivery optics and is limited by ocular aberrations. Through the combination of wavefront sensorless adaptive optics and the use of dual deformable transmissive optical elements, we present a compact lens-based OCT system at an imaging wavelength of 1060 nm for high resolution retinal imaging. We utilized a commercially available variable focal length lens to correct for a wide range of defocus commonly found in patient’s eyes, and a novel multi-actuator adaptive lens for aberration correction to achieve near diffraction limited imaging performance at the retina. With a parallel processing computational platform, high resolution cross-sectional and en face retinal image acquisition and display was performed in real time. In order to demonstrate the system functionality and clinical utility, we present images of the photoreceptor cone mosaic and other retinal layers acquired in vivo from research subjects.

  15. THE GRAY NEEDLE: LARGE GRAINS IN THE HD 15115 DEBRIS DISK FROM LBT /PISCES/Ks AND LBTI /LMIRcam/L' ADAPTIVE OPTICS IMAGING

    SciTech Connect

    Rodigas, Timothy J.; Hinz, Philip M.; Vaitheeswaran, Vidhya; Skemer, Andrew J.; Su, Kate Y. L.; Bailey, Vanessa; Schneider, Glenn; Close, Laird; Apai, Daniel; Leisenring, Jarron; Skrutskie, Michael; Mannucci, Filippo; Esposito, Simone; Arcidiacono, Carmelo; Pinna, Enrico; Argomedo, Javier; Agapito, Guido; Bono, Giuseppe; Briguglio, Runa; Boutsia, Kostantina; and others

    2012-06-10

    We present diffraction-limited Ks band and L' adaptive optics images of the edge-on debris disk around the nearby F2 star HD 15115, obtained with a single 8.4 m primary mirror at the Large Binocular Telescope. At the Ks band, the disk is detected at signal-to-noise per resolution element (SNRE) {approx} 3-8 from {approx}1 to 2.''5 (45-113 AU) on the western side and from {approx}1.''2 to 2.''1 (63-90 AU) on the east. At L' the disk is detected at SNRE {approx} 2.5 from {approx}1 to 1.''45 (45-90 AU) on both sides, implying more symmetric disk structure at 3.8 {mu}m. At both wavelengths the disk has a bow-like shape and is offset from the star to the north by a few AU. A surface brightness asymmetry exists between the two sides of the disk at the Ks band, but not at L'. The surface brightness at the Ks band declines inside 1'' ({approx}45 AU), which may be indicative of a gap in the disk near 1''. The Ks - L' disk color, after removal of the stellar color, is mostly gray for both sides of the disk. This suggests that scattered light is coming from large dust grains, with 3-10 {mu}m sized grains on the east side and 1-10 {mu}m dust grains on the west. This may suggest that the west side is composed of smaller dust grains than the east side, which would support the interpretation that the disk is being dynamically affected by interactions with the local interstellar medium.

  16. Adaptive image segmentation by quantization

    NASA Astrophysics Data System (ADS)

    Liu, Hui; Yun, David Y.

    1992-12-01

    Segmentation of images into textural homogeneous regions is a fundamental problem in an image understanding system. Most region-oriented segmentation approaches suffer from the problem of different thresholds selecting for different images. In this paper an adaptive image segmentation based on vector quantization is presented. It automatically segments images without preset thresholds. The approach contains a feature extraction module and a two-layer hierarchical clustering module, a vector quantizer (VQ) implemented by a competitive learning neural network in the first layer. A near-optimal competitive learning algorithm (NOLA) is employed to train the vector quantizer. NOLA combines the advantages of both Kohonen self- organizing feature map (KSFM) and K-means clustering algorithm. After the VQ is trained, the weights of the network and the number of input vectors clustered by each neuron form a 3- D topological feature map with separable hills aggregated by similar vectors. This overcomes the inability to visualize the geometric properties of data in a high-dimensional space for most other clustering algorithms. The second clustering algorithm operates in the feature map instead of the input set itself. Since the number of units in the feature map is much less than the number of feature vectors in the feature set, it is easy to check all peaks and find the `correct' number of clusters, also a key problem in current clustering techniques. In the experiments, we compare our algorithm with K-means clustering method on a variety of images. The results show that our algorithm achieves better performance.

  17. Holographic fluorescence microscopy with incoherent digital holographic adaptive optics

    NASA Astrophysics Data System (ADS)

    Jang, Changwon; Kim, Jonghyun; Clark, David C.; Lee, Byoungho; Kim, Myung K.

    2015-03-01

    Introduction of adaptive optics technology into astronomy and ophthalmology has made great contributions in these fields, allowing one to recover images blurred by atmospheric turbulence or aberrations of the eye. Similar adaptive optics improvement in microscopic imaging is also of interest to researchers using various techniques. Current technology of adaptive optics typically contains three key elements: wavefront sensor, wavefront corrector and controller. These hardware elements tend to be bulky, expensive, and limited in resolution, involving, e.g., lenslet arrays for sensing or multi-acuator deformable mirrors for correcting. We have previously introduced an alternate approach to adaptive optics based on unique capabilities of digital holography, namely direct access to the phase profile of an optical field and the ability to numerically manipulate the phase profile. We have also demonstrated that direct access and compensation of the phase profile is possible not only with the conventional coherent type of digital holography, but also with a new type of digital holography using incoherent light: self-interference incoherent digital holography (SIDH). The SIDH generates complex - i.e. amplitude plus phase - hologram from one or several interferograms acquired with incoherent light, such as LEDs, lamps, sunlight, or fluorescence. The complex point spread function can be measured using a guide star illumination and it allows deterministic deconvolution of the full-field image. We present experimental demonstration of aberration compensation in holographic fluorescence microscopy using SIDH. The adaptive optics by SIDH provides new tools for improved cellular fluorescence microscopy through intact tissue layers or other types of aberrant media.

  18. Adaptive optics confocal microscopy using direct wavefront sensing.

    PubMed

    Tao, Xiaodong; Fernandez, Bautista; Azucena, Oscar; Fu, Min; Garcia, Denise; Zuo, Yi; Chen, Diana C; Kubby, Joel

    2011-04-01

    Optical aberrations due to the inhomogeneous refractive index of tissue degrade the resolution and brightness of images in deep-tissue imaging. We introduce a confocal fluorescence microscope with adaptive optics, which can correct aberrations based on direct wavefront measurements using a Shack-Hartmann wavefront sensor with a fluorescent bead used as a point source reference beacon. The results show a 4.3× improvement in the Strehl ratio and a 240% improvement in the signal intensity for fixed mouse tissues at depths of up to 100 μm.

  19. Thin nearly wireless adaptive optical device

    NASA Technical Reports Server (NTRS)

    Knowles, Gareth J. (Inventor); Hughes, Eli (Inventor)

    2009-01-01

    A thin nearly wireless adaptive optical device capable of dynamically modulating the shape of a mirror in real time to compensate for atmospheric distortions and/or variations along an optical material is provided. The device includes an optical layer, a substrate, at least one electronic circuit layer with nearly wireless architecture, an array of actuators, power electronic switches, a reactive force element, and a digital controller. Actuators are aligned so that each axis of expansion and contraction intersects both substrate and reactive force element. Electronics layer with nearly wireless architecture, power electronic switches, and digital controller are provided within a thin-film substrate. The size and weight of the adaptive optical device is solely dominated by the size of the actuator elements rather than by the power distribution system.

  20. Thin, nearly wireless adaptive optical device

    NASA Technical Reports Server (NTRS)

    Knowles, Gareth (Inventor); Hughes, Eli (Inventor)

    2008-01-01

    A thin, nearly wireless adaptive optical device capable of dynamically modulating the shape of a mirror in real time to compensate for atmospheric distortions and/or variations along an optical material is provided. The device includes an optical layer, a substrate, at least one electronic circuit layer with nearly wireless architecture, an array of actuators, power electronic switches, a reactive force element, and a digital controller. Actuators are aligned so that each axis of expansion and contraction intersects both substrate and reactive force element. Electronics layer with nearly wireless architecture, power electronic switches, and digital controller are provided within a thin-film substrate. The size and weight of the adaptive optical device is solely dominated by the size of the actuator elements rather than by the power distribution system.

  1. Thin, nearly wireless adaptive optical device

    NASA Technical Reports Server (NTRS)

    Knowles, Gareth (Inventor); Hughes, Eli (Inventor)

    2007-01-01

    A thin, nearly wireless adaptive optical device capable of dynamically modulating the shape of a mirror in real time to compensate for atmospheric distortions and/or variations along an optical material is provided. The device includes an optical layer, a substrate, at least one electronic circuit layer with nearly wireless architecture, an array of actuators, power electronic switches, a reactive force element, and a digital controller. Actuators are aligned so that each axis of expansion and contraction intersects both substrate and reactive force element. Electronics layer with nearly wireless architecture, power electronic switches, and digital controller are provided within a thin-film substrate. The size and weight of the adaptive optical device is solely dominated by the size of the actuator elements rather than by the power distribution system.

  2. Acousto-Optical Imaging Spectropolarimeter

    NASA Technical Reports Server (NTRS)

    Saif, Babak; Glenar, David; Zimmerman, Robert; Seery, Bernard

    1992-01-01

    Imaging spectropolarimeter designed around acousto-optical tunable filter (AOTF) takes polarization-specific spectral images of solid surfaces, aerosols, and absorption and emission phenomena in gas phase, at wavelengths from 500 to 1,000 nm. Produces side-by-side spectral images in two mutually perpendicular polarizations, one corresponding to ordinary, other corresponding to extraordinary waves in acousto-optical material. Offers large aperture, high resolving power, and rapid tunability, with no moving parts.

  3. Characterization and Operation of Liquid Crystal Adaptive Optics Phoropter

    SciTech Connect

    Awwal, A; Bauman, B; Gavel, D; Olivier, S; Jones, S; Hardy, J L; Barnes, T; Werner, J S

    2003-02-05

    Adaptive optics (AO), a mature technology developed for astronomy to compensate for the effects of atmospheric turbulence, can also be used to correct the aberrations of the eye. The classic phoropter is used by ophthalmologists and optometrists to estimate and correct the lower-order aberrations of the eye, defocus and astigmatism, in order to derive a vision correction prescription for their patients. An adaptive optics phoropter measures and corrects the aberrations in the human eye using adaptive optics techniques, which are capable of dealing with both the standard low-order aberrations and higher-order aberrations, including coma and spherical aberration. High-order aberrations have been shown to degrade visual performance for clinical subjects in initial investigations. An adaptive optics phoropter has been designed and constructed based on a Shack-Hartmann sensor to measure the aberrations of the eye, and a liquid crystal spatial light modulator to compensate for them. This system should produce near diffraction-limited optical image quality at the retina, which will enable investigation of the psychophysical limits of human vision. This paper describes the characterization and operation of the AO phoropter with results from human subject testing.

  4. Free Space Optical Communications Utilizing MEMS Adaptive Optics Correction

    SciTech Connect

    Thompson, C A; Kartz, M W; Flath, L M; Wilks, S C; Young, R A; Johnson, G W; Ruggiero, A J

    2002-07-09

    Free space optical communications (FSO) are beginning to provide attractive alternatives to fiber-based solutions in many situations. Currently, a handful of companies provide fiberless alternatives specifically aimed at corporate intranet and sporting event video applications. These solutions are geared toward solving the ''last mile'' connectivity issues. There exists a potential need to extend this pathlength to distances much greater than a 1 km, particularly for government and military applications. For cases of long distance optical propagation, atmospheric turbulence will ultimately limit the maximum achievable data rate. In this paper, we propose a method to improve signal quality through the use of adaptive optics. In particular, we show work in progress toward a high-speed, small footprint Adaptive Optics system for horizontal and slant path laser communications. Such a system relies heavily on recent progress in Micro-Electro-Mechanical Systems (MEMS) deformable mirrors, as well as improved communication and computational components.

  5. Adaptive-clustering optical neural net.

    PubMed

    Casasent, D P; Barnard, E

    1990-06-10

    Pattern recognition techniques (for clustering and linear discriminant function selection) are combined with neural net methods (that provide an automated method to combine linear discriminant functions into piecewise linear discriminant surfaces). The resulting adaptive-clustering neural net is suitable for optical implementation and has certain desirable properties in comparison with other neural nets. Simulation results are provided.

  6. Extragalactic Fields Optimized for Adaptive Optics

    NASA Astrophysics Data System (ADS)

    Damjanov, Ivana; Abraham, Roberto G.; Glazebrook, Karl; McGregor, Peter; Rigaut, Francois; McCarthy, Patrick J.; Brinchmann, Jarle; Cuillandre, Jean-Charles; Mellier, Yannick; McCracken, Henry Joy; Hudelot, Patrick; Monet, David

    2011-03-01

    In this article we present the coordinates of 67 55' × 55' patches of sky that have the rare combination of both high stellar surface density (>=0.5 arcmin-2 with 13 < R < 16.5 mag) and low extinction (E(B - V)<=0.1). These fields are ideal for adaptive-optics-based follow-up of extragalactic targets. One region of sky, situated near Baade's Window, contains most of the patches we have identified. Our optimal field, centered at R.A.: 7h24m3s, decl.: -1°27'15'', has an additional advantage of being accessible from both hemispheres. We propose a figure of merit for quantifying real-world adaptive optics performance and use this to analyze the performance of multiconjugate adaptive optics in these fields. We also compare our results with those that would be obtained in existing deep fields. In some cases adaptive optics observations undertaken in the fields given in this article would be orders of magnitude more efficient than equivalent observations undertaken in existing deep fields.

  7. Teaching Optics and Systems Engineering With Adaptive Optics Workbenches

    NASA Astrophysics Data System (ADS)

    Harrington, D. M.; Ammons, M.; Hunter, L.; Max, C.; Hoffmann, M.; Pitts, M.; Armstrong, J. D.

    2010-12-01

    Adaptive optics workbenches are fully functional optical systems that can be used to illustrate and teach a variety of concepts and cognitive processes. Four systems have been funded, designed and constructed by various institutions and people as part of education programs associated with the Center for Adaptive Optics, the Professional Development Program and the Institute for Scientist & Engineer Educators. Activities can range from first-year undergraduate explorations to professional level training. These workbenches have been used in many venues including the Center for Adaptive Optics AO Summer School, the Maui Community College-hosted Akamai Maui Short Course, classrooms, training of new staff in laboratories and other venues. The activity content has focused on various elements of systems thinking, characterization, feedback and system control, basic optics and optical alignment as well as advanced topics such as phase conjugation, wave-front sensing and correction concepts, and system design. The workbenches have slightly different designs and performance capabilities. We describe here outlines for several activities utilizing these different designs and some examples of common student learner outcomes and experiences.

  8. Optical axis jitter rejection for double overlapped adaptive optics systems

    NASA Astrophysics Data System (ADS)

    Luo, Qi; Luo, Xi; Li, Xinyang

    2016-04-01

    Optical axis jitters, or vibrations, which arise from wind shaking and structural oscillations of optical platforms, etc., cause a deleterious impact on the performance of adaptive optics systems. When conventional integrators are utilized to reject such high frequency and narrow-band disturbance, the benefits are quite small despite their acceptable capabilities to reject atmospheric turbulence. In our case, two suits of complete adaptive optics systems called double overlapped adaptive optics systems (DOAOS) are used to counteract both optical jitters and atmospheric turbulence. A novel algorithm aiming to remove vibrations is proposed by resorting to combine the Smith predictor and notch filer. With the help of loop shaping method, the algorithm will lead to an effective and stable controller, which makes the characteristics of error transfer function close to notch filters. On the basis of the spectral analysis of observed data, the peak frequency and bandwidth of vibrations can be identified in advance. Afterwards, the number of notch filters and their parameters will be determined using coordination descending method. The relationship between controller parameters and filtering features is discussed, and the robustness of the controller against varying parameters of the control object is investigated. Preliminary experiments are carried out to validate the proposed algorithms. The overall control performance of DOAOS is simulated. Results show that time delays are a limit of the performance, but the algorithm can be successfully implemented on our systems, which indicate that it has a great potential to reject jitters.

  9. Optimization-based wavefront sensorless adaptive optics for multiphoton microscopy.

    PubMed

    Antonello, Jacopo; van Werkhoven, Tim; Verhaegen, Michel; Truong, Hoa H; Keller, Christoph U; Gerritsen, Hans C

    2014-06-01

    Optical aberrations have detrimental effects in multiphoton microscopy. These effects can be curtailed by implementing model-based wavefront sensorless adaptive optics, which only requires the addition of a wavefront shaping device, such as a deformable mirror (DM) to an existing microscope. The aberration correction is achieved by maximizing a suitable image quality metric. We implement a model-based aberration correction algorithm in a second-harmonic microscope. The tip, tilt, and defocus aberrations are removed from the basis functions used for the control of the DM, as these aberrations induce distortions in the acquired images. We compute the parameters of a quadratic polynomial that is used to model the image quality metric directly from experimental input-output measurements. Finally, we apply the aberration correction by maximizing the image quality metric using the least-squares estimate of the unknown aberration.

  10. Adaptive optics at the Subaru telescope: current capabilities and development

    NASA Astrophysics Data System (ADS)

    Guyon, Olivier; Hayano, Yutaka; Tamura, Motohide; Kudo, Tomoyuki; Oya, Shin; Minowa, Yosuke; Lai, Olivier; Jovanovic, Nemanja; Takato, Naruhisa; Kasdin, Jeremy; Groff, Tyler; Hayashi, Masahiko; Arimoto, Nobuo; Takami, Hideki; Bradley, Colin; Sugai, Hajime; Perrin, Guy; Tuthill, Peter; Mazin, Ben

    2014-08-01

    Current AO observations rely heavily on the AO188 instrument, a 188-elements system that can operate in natural or laser guide star (LGS) mode, and delivers diffraction-limited images in near-IR. In its LGS mode, laser light is transported from the solid state laser to the launch telescope by a single mode fiber. AO188 can feed several instruments: the infrared camera and spectrograph (IRCS), a high contrast imaging instrument (HiCIAO) or an optical integral field spectrograph (Kyoto-3DII). Adaptive optics development in support of exoplanet observations has been and continues to be very active. The Subaru Coronagraphic Extreme-AO (SCExAO) system, which combines extreme-AO correction with advanced coronagraphy, is in the commissioning phase, and will greatly increase Subaru Telescope's ability to image and study exoplanets. SCExAO currently feeds light to HiCIAO, and will soon be combined with the CHARIS integral field spectrograph and the fast frame MKIDs exoplanet camera, which have both been specifically designed for high contrast imaging. SCExAO also feeds two visible-light single pupil interferometers: VAMPIRES and FIRST. In parallel to these direct imaging activities, a near-IR high precision spectrograph (IRD) is under development for observing exoplanets with the radial velocity technique. Wide-field adaptive optics techniques are also being pursued. The RAVEN multi-object adaptive optics instrument was installed on Subaru telescope in early 2014. Subaru Telescope is also planning wide field imaging with ground-layer AO with the ULTIMATE-Subaru project.

  11. Melanoma associated retinopathy: A new dimension using adaptive optics.

    PubMed

    Dabir, Supriya; Mangalesh, Shwetha; Govindraj, Indu; Mallipatna, Ashwin; Battu, Rajani; Shetty, Rohit

    2015-01-01

    We report a 56-year-old male patient, complaining of metamorphopsia in his left eye nevertheless visual acuity, slit lamp, and fundus examinations were within normal limits. Microperimetry (MAIA, Centervue, Italy) revealed central field loss and spectral domain optical coherence tomography (Spectralis, Heidelberg, Germany) showed disrupted cone outer segment tip layer. The patient had a diagnosis of cutaneous melanoma in his foot for which an excision biopsy with lymph node dissection was performed 5 months earlier. Our clinical diagnosis was melanoma-associated retinopathy. Electrophysiology confirmed the diagnosis. Adaptive optics retinal imaging (Imagine eyes, Orsay) was performed to assess the cone mosaic integrity across the central retina. This is the first report on the investigation of autoimmune retinopathy using adaptive optics ophthalmoscopy. This case highlights the viability of innovative diagnostic modalities that aid early detection and subsequent management of vision threatening retinal.

  12. Adaptive Optics Photometry and Astrometry of Binary Stars

    NASA Astrophysics Data System (ADS)

    Roberts, Lewis C., Jr.; Turner, Nils H.; Bradford, L. William; ten Brummelaar, Theo A.; Oppenheimer, Ben R.; Kuhn, Jeff R.; Whitman, Kathryn; Perrin, Marshall D.; Graham, James R.

    2005-11-01

    We present astrometric and photometric measurements of 39 binary stars made with the adaptive optics system on the 3.6 m Advanced Electro-Optical System (AEOS) telescope, taken from 2002 November to 2003 March. The binaries have separations ranging from 0.08" to 5.11" and differential magnitudes ranging from 0.096 to 7.9. Also, we include a list of observations of 23 known binaries that we were unable to resolve. In the process of these measurements, we discovered three new companions to two previously known binary stars. We also discuss the effects of scintillation and anisoplanatism on measurements of binary star photometry in adaptive optics images. Suggestions on how to minimize these effects are then given. Based on observations made at the Maui Space Surveillance System operated by Detachment 15 of the US Air Force Research Laboratory's Directed Energy Directorate.

  13. POPART: partial optical implementation of adaptive resonance theory 2.

    PubMed

    Kane, J S; Paquin, M J

    1993-01-01

    Adaptive resonance architectures are neural nets that are capable of classifying arbitrary input patterns into stable category representations. A hybrid optoelectronic implementation utilizing an optical joint transform correlator is proposed and demonstrated. The resultant optoelectronic system is able to reduce the number of calculations compared to a strictly computer-based approach. The result is that, for larger images, the optoelectronic system is faster than the computer-based approach.

  14. Adaptive holography for optical sensing applications

    NASA Astrophysics Data System (ADS)

    Residori, S.; Bortolozzo, U.; Peigné, A.; Molin, S.; Nouchi, P.; Dolfi, D.; Huignard, J. P.

    2016-03-01

    Adaptive holography is a promising method for high sensitivity phase modulation measurements in the presence of slow perturbations from the environment. The technique is based on the use of a nonlinear recombining medium, here an optically addressed spatial light modulator specifically realized to operate at 1.55 μm. Owing to the physical mechanisms involved, the interferometer adapts to slow phase variations within a range of 5-10 Hz, thus filtering out low frequency noise while transmitting higher frequency phase modulations. We present the basic principles of the adaptive interferometer and show that it can be used in association with a sensing fiber in order to detect phase modulations. Finally, a phase-OTDR architecture using the adaptive holographic interferometer is presented and shown to allows the detection of localized perturbations along the sensing fiber.

  15. Development of large aperture composite adaptive optics

    NASA Astrophysics Data System (ADS)

    Kmetik, Viliam; Vitovec, Bohumil; Jiran, Lukas; Nemcova, Sarka; Zicha, Josef; Inneman, Adolf; Mikulickova, Lenka; Pavlica, Richard

    2015-01-01

    Large aperture composite adaptive optics for laser applications is investigated in cooperation of Institute of Plasma Physic, Department of Instrumentation and Control Engineering FME CTU and 5M Ltd. We are exploring opportunity of a large-size high-power-laser deformable-mirror production using a lightweight bimorph actuated structure with a composite core. In order to produce a sufficiently large operational free aperture we are developing new technologies for production of flexible core, bimorph actuator and deformable mirror reflector. Full simulation of a deformable-mirrors structure was prepared and validated by complex testing. A deformable mirror actuation and a response of a complicated structure are investigated for an accurate control of the adaptive optics. An original adaptive optics control system and a bimorph deformable mirror driver were developed. Tests of material samples, components and sub-assemblies were completed. A subscale 120 mm bimorph deformable mirror prototype was designed, fabricated and thoroughly tested. A large-size 300 mm composite-core bimorph deformable mirror was simulated and optimized, fabrication of a prototype is carried on. A measurement and testing facility is modified to accommodate large sizes optics.

  16. Active optics, adaptive optics, and laser guide stars.

    PubMed

    Hubin, N; Noethe, L

    1993-11-26

    Optical astronomy is crucial to our understanding of the universe, but the capabilities of ground-based telescopes are severely limited by the effects of telescope errors and of the atmosphere on the passage of light. Recently, it has become possible to construct inbuilt corrective devices that can compensate for both types of degradations as observations are conducted. For full use of the newly emerged class of 8-meter telescopes, such active corrective capabilities, known as active and adaptive optics, are essential. Some physical limitations in the adaptive optics field can be overcome by artificially created reference stars, called laser guide stars. These new technologies have lately been applied with success to some medium and very large telescopes. PMID:17736819

  17. Simulating Astronomical Adaptive Optics Systems Using Yao

    NASA Astrophysics Data System (ADS)

    Rigaut, François; Van Dam, Marcos

    2013-12-01

    Adaptive Optics systems are at the heart of the coming Extremely Large Telescopes generation. Given the importance, complexity and required advances of these systems, being able to simulate them faithfully is key to their success, and thus to the success of the ELTs. The type of systems envisioned to be built for the ELTs cover most of the AO breeds, from NGS AO to multiple guide star Ground Layer, Laser Tomography and Multi-Conjugate AO systems, with typically a few thousand actuators. This represents a large step up from the current generation of AO systems, and accordingly a challenge for existing AO simulation packages. This is especially true as, in the past years, computer power has not been following Moore's law in its most common understanding; CPU clocks are hovering at about 3GHz. Although the use of super computers is a possible solution to run these simulations, being able to use smaller machines has obvious advantages: cost, access, environmental issues. By using optimised code in an already proven AO simulation platform, we were able to run complex ELT AO simulations on very modest machines, including laptops. The platform is YAO. In this paper, we describe YAO, its architecture, its capabilities, the ELT-specific challenges and optimisations, and finally its performance. As an example, execution speed ranges from 5 iterations per second for a 6 LGS 60x60 subapertures Shack-Hartmann Wavefront sensor Laser Tomography AO system (including full physical image formation and detector characteristics) up to over 30 iterations/s for a single NGS AO system.

  18. Isoplanatism in a multiconjugate adaptive optics system.

    PubMed

    Tokovinin, A; Le Louarn, M; Sarazin, M

    2000-10-01

    Turbulence correction in a large field of view by use of an adaptive optics imaging system with several deformable mirrors (DM's) conjugated to various heights is considered. The residual phase variance is computed for an optimized linear algorithm in which a correction of each turbulent layer is achieved by applying a combination of suitably smoothed and scaled input phase screens to all DM's. Finite turbulence outer scale and finite spatial resolution of the DM's are taken into account. A general expression for the isoplanatic angle thetaM of a system with M mirrors is derived in the limiting case of infinitely large apertures and Kolmogorov turbulence. Like Fried's isoplanatic angle theta0,thetaM is a function only of the turbulence vertical profile, is scalable with wavelength, and is independent of the telescope diameter. Use of angle thetaM permits the gain in the field of view due to the increased number of DM's to be quantified and their optimal conjugate heights to be found. Calculations with real turbulence profiles show that with three DM's a gain of 7-10x is possible, giving the typical and best isoplanatic field-of-view radii of 16 and 30 arcseconds, respectively, at lambda = 0.5 microm. It is shown that in the actual systems the isoplanatic field will be somewhat larger than thetaM owing to the combined effects of finite aperture diameter, finite outer scale, and optimized wave-front spatial filtering. However, this additional gain is not dramatic; it is less than 1.5x for large-aperture telescopes. PMID:11028530

  19. Isoplanatism in a multiconjugate adaptive optics system.

    PubMed

    Tokovinin, A; Le Louarn, M; Sarazin, M

    2000-10-01

    Turbulence correction in a large field of view by use of an adaptive optics imaging system with several deformable mirrors (DM's) conjugated to various heights is considered. The residual phase variance is computed for an optimized linear algorithm in which a correction of each turbulent layer is achieved by applying a combination of suitably smoothed and scaled input phase screens to all DM's. Finite turbulence outer scale and finite spatial resolution of the DM's are taken into account. A general expression for the isoplanatic angle thetaM of a system with M mirrors is derived in the limiting case of infinitely large apertures and Kolmogorov turbulence. Like Fried's isoplanatic angle theta0,thetaM is a function only of the turbulence vertical profile, is scalable with wavelength, and is independent of the telescope diameter. Use of angle thetaM permits the gain in the field of view due to the increased number of DM's to be quantified and their optimal conjugate heights to be found. Calculations with real turbulence profiles show that with three DM's a gain of 7-10x is possible, giving the typical and best isoplanatic field-of-view radii of 16 and 30 arcseconds, respectively, at lambda = 0.5 microm. It is shown that in the actual systems the isoplanatic field will be somewhat larger than thetaM owing to the combined effects of finite aperture diameter, finite outer scale, and optimized wave-front spatial filtering. However, this additional gain is not dramatic; it is less than 1.5x for large-aperture telescopes.

  20. Configurable adaptive optics for the correction of space-based optical systems

    NASA Astrophysics Data System (ADS)

    McComas, Brian Keith

    Space-based, high resolution, Earth remote sensing systems, that employ large, flexible, lightweight primary mirrors, will require active wavefront correction, in the form of active and adaptive optics, to correct for thermally and vibrationally induced deformations in the optics. These remote sensing systems typically have a large field-of-view. Unlike the adaptive optics on ground-based astronomical telescopes, which have a negligible field-of-view, the adaptive optics on these space-based remote sensing systems will be required to correct the wavefront over the entire field-of-view, which can be several degrees. The error functions for astronomical adaptive optics have been developed for the narrow field-of-view correction of atmospheric turbulence and do not address the needs of wide field space-based systems. To address these needs, a new wide field adaptive optics theory and a new error function are developed. This new error function, which is a new extension of conventional adaptive optics, leads to the development of three new types of imaging systems: wide field-of-view, selectable field-of-view, and steerable field-of-view. These new systems can have nearly diffraction-limited performance across the entire field-of-view or a narrow movable region of high-resolution imaging. The factors limiting system performance are determined and analyzed. The range of applicability of the wide field adaptive optics theory is shown. The range of applicability is used to avoid limitations in system performance and to estimate the optical systems parameters, which will meet the system's performance requirements. Experimental results demonstrate the wide field adaptive optics theory. Finally, it will be shown that a synthetic guide star stimulated from above the atmosphere can be used as a beacon for the wavefront sensors of space-based systems. These wavefront sensors must be optimized such that error in the reconstructed wavefront is minimized. The key equations that

  1. Performance of a MEMS-base Adaptive Optics Optical Coherency Tomography System

    SciTech Connect

    Evans, J; Zadwadzki, R J; Jones, S; Olivier, S; Opkpodu, S; Werner, J S

    2008-01-16

    We have demonstrated that a microelectrical mechanical systems (MEMS) deformable mirror can be flattened to < 1 nm RMS within controllable spatial frequencies over a 9.2-mm aperture making it a viable option for high-contrast adaptive optics systems (also known as Extreme Adaptive Optics). The Extreme Adaptive Optics Testbed at UC Santa Cruz is being used to investigate and develop technologies for high-contrast imaging, especially wavefront control. A phase shifting diffraction interferometer (PSDI) measures wavefront errors with sub-nm precision and accuracy for metrology and wavefront control. Consistent flattening, required testing and characterization of the individual actuator response, including the effects of dead and low-response actuators. Stability and repeatability of the MEMS devices was also tested. An error budget for MEMS closed loop performance will summarize MEMS characterization.

  2. Diffractive-optical correlators: chances to make optical image preprocessing as intelligent as human vision

    NASA Astrophysics Data System (ADS)

    Lauinger, Norbert

    2004-10-01

    The human eye is a good model for the engineering of optical correlators. Three prominent intelligent functionalities in human vision could in the near future become realized by a new diffractive-optical hardware design of optical imaging sensors: (1) Illuminant-adaptive RGB-based color Vision, (2) Monocular 3D Vision based on RGB data processing, (3) Patchwise fourier-optical Object-Classification and Identification. The hardware design of the human eye has specific diffractive-optical elements (DOE's) in aperture and in image space and seems to execute the three jobs at -- or not far behind -- the loci of the images of objects.

  3. Discrete adaptive zone light elements (DAZLE): a new approach to adaptive imaging

    NASA Astrophysics Data System (ADS)

    Kellogg, Robert L.; Escuti, Michael J.

    2007-09-01

    New advances in Liquid Crystal Spatial Light Modulators (LCSLM) offer opportunities for large adaptive optics in the midwave infrared spectrum. A light focusing adaptive imaging system, using the zero-order diffraction state of a polarizer-free liquid crystal polarization grating modulator to create millions of high transmittance apertures, is envisioned in a system called DAZLE (Discrete Adaptive Zone Light Elements). DAZLE adaptively selects large sets of LCSLM apertures using the principles of coded masks, embodied in a hybrid Discrete Fresnel Zone Plate (DFZP) design. Issues of system architecture, including factors of LCSLM aperture pattern and adaptive control, image resolution and focal plane array (FPA) matching, and trade-offs between filter bandwidths, background photon noise, and chromatic aberration are discussed.

  4. Extended depth of focus adaptive optics spectral domain optical coherence tomography

    PubMed Central

    Sasaki, Kazuhiro; Kurokawa, Kazuhiro; Makita, Shuichi; Yasuno, Yoshiaki

    2012-01-01

    We present an adaptive optics spectral domain optical coherence tomography (AO-SDOCT) with a long focal range by active phase modulation of the pupil. A long focal range is achieved by introducing AO-controlled third-order spherical aberration (SA). The property of SA and its effects on focal range are investigated in detail using the Huygens-Fresnel principle, beam profile measurement and OCT imaging of a phantom. The results indicate that the focal range is extended by applying SA, and the direction of extension can be controlled by the sign of applied SA. Finally, we demonstrated in vivo human retinal imaging by altering the applied SA. PMID:23082278

  5. Effectiveness of adaptive optics system in satellite-to-ground coherent optical communication.

    PubMed

    Jian, Huang; Ke, Deng; Chao, Liu; Peng, Zhang; Dagang, Jiang; Zhoushi, Yao

    2014-06-30

    Adaptive optics (AO) systems can suppress the signal fade induced by atmospheric turbulence in satellite-to-ground coherent optical communication. The lower bound of the signal fade under AO compensation was investigated by analyzing the pattern of aberration modes for a one-stage imaging AO system. The distribution of the root mean square of the residual aberration is discussed on the basis of the spatial and temporal characteristics of the residual aberration of the AO system. The effectiveness of the AO system for improving the performance of coherent optical communication is presented in terms of the bit error rate and system availability.

  6. Thermo-optically driven adaptive mirror

    NASA Astrophysics Data System (ADS)

    Reinert, Felix; Lüthy, Willy

    2006-02-01

    The ideal adaptive optical mirror combines large aperture with high spatial and temporal resolution and a phase shift of at least 2π. Further, a simple low-cost solution is preferred. No adaptive system can perfectly fulfill all these requirements. We present a system that has the potential to reach this goal with the exception of high temporal resolution. But even with a moderate temporal resolution of one second such a system can find practical applications. For example as a laser resonator mirror that allows to modify the intensity distribution of the emission, or to correct slowly varying aberrations of optical systems. Two possible mechanisms can be used to change the optical path length of the adaptive mirror: thermal expansion of the mirror substrate or the thermally induced change of the refractive index (thermal dispersion) of a medium in front of the mirror. Both mechanisms have been shown to lead to promising results. In both cases heating was performed by irradiation of light in the active medium. The thermal dispersion based adaptive mirror is built with a thin layer of a liquid in front of a mirror. To allow a modification of the refractive index by irradiation with a diode laser at 808 nm, a suitable absorber is dissolved in the water. With chopped irradiation a resolution of 3.8 Hz at 30 % contrast is measured. This mirror has been used in a laser resonator to modify the output distribution of the laser. The thermal expansion based adaptive mirror is built with a thin layer of a silicon elastomer with a gold coated front side. We present a preparation method to produce thin films of Sylgard on sapphire. With an irradiated intensity of only 370 mW/cm2 surface modulations of up to 350 nm are obtained. With a test pattern a resolution of 1.6 line-pairs per millimeter at 30 % contrast is measured. The temporal resolution is better than one second.

  7. Supernovae and extragalactic astronomy with laser guide star adaptive optics

    NASA Astrophysics Data System (ADS)

    Ryder, Stuart D.; Mattila, Seppo; Kankare, Erkki; Väisänen, Petri

    2014-07-01

    Using the latest generation of adaptive optics imaging systems together with laser guide stars on 8m-class telescopes, we are finally revealing the previously-hidden population of supernovae in starburst galaxies. Finding these supernovae and measuring the amount of absorption due to dust is crucial to being able to accurately trace the star formation history of our Universe. Our images are amongst the sharpest ever obtained from the ground, and reveal much about how and why these galaxies are forming massive stars (that become supernovae) at such a prodigious rate.

  8. The AVES adaptive optics spectrograph for the VLT: status report

    NASA Astrophysics Data System (ADS)

    Pallavicini, Roberto; Delabre, Bernard; Pasquini, Luca; Zerbi, Filippo M.; Bonanno, Giovanni; Comari, Maurizio; Conconi, Paolo; Mazzoleni, Ruben; Santin, Paolo; Damiani, Francesco; Di Marcantonio, Paolo; Franchini, Mariagrazia; Spano, Paolo; Bonifacio, P.; Catalano, Santo; Molaro, Paolo P.; Randich, S.; Rodono, Marcello

    2003-03-01

    We report on the status of AVES, the Adaptive-optics Visual Echelle Spectrograph proposed for the secondary port of the Nasmyth Adaptive Optics System (NAOS) recently installed at the VLT. AVES is an intermediate resolution (R ≍ 16,000) high-efficiency fixed- format echelle spectrograph which operates in the spectral band 500 - 1,000 nm. In addition to a high intrinsic efficiency, comparable to that of ESI at Keck II, it takes advantage of the adaptive optics correction provided by NAOS to reduce the sky and detector contribution in background-limited observations of weak sources, thus allowing a further magnitude gain with respect to comparable non-adaptive optics spectrographs. Simulations show that the instrument will be capable of reaching a magnitude V = 22.5 at S/N > 10 in two hours, two magnitudes weaker than GIRAFFE at the same resolution and 3 magnitudes weaker than the higher resolution UVES spectrograph. Imaging and coronographic functions have also been implemented in the design. We present the results of the final design study and we dicuss the technical and operational issues related to its implementation at the VLT as a visitor instrument. We also discuss the possibility of using a scaled-up non-adaptive optics version of the same design as an element of a double- or triple-arm intermediate-resolution spectrograph for the VLT. Such an option looks attractive in the context of a high-efficiency large-bandwidth (320 - 1,500 nm) spectrograph ("fast-shooter") being considered by ESO as a 2nd-generation VLT instrument.

  9. Specialized wavefront sensors for adaptive optics

    NASA Astrophysics Data System (ADS)

    Neal, Daniel R.; Mansell, J. D.; Gruetzner, James K.; Morgan, R.; Warren, Mial E.

    1995-08-01

    The performance of an adaptive optical system is strongly dependent upon correctly measuring the wavefront of the arriving light. The most common wavefront measurement techniques used to date are the shearing interferometer and the Shack-Hartmann sensor. Shack-Hartmann sensors rely on the use of lenslet arrays to sample the aperture appropriately. These have traditionally been constructed using MLM or step and repeat technology, and more recently with binary optics technology. Diffractive optics fabrication methodology can be used to remove some of the limitations of the previous technologies and can allow for low-cost production of sophisticated elements. We have investigated several different specialized wavefront sensor configurations using both Shack-Hartmann and shearing interferometer principles. We have taken advantage of the arbitrary nature of these elements to match pupil shapes of detector and telescope aperture and to introduce magnification between the lenslet array and the detector. We have fabricated elements that facilitate matching the sampling to the current atmospheric conditions. The sensors were designed using a far-field diffraction model and a photolithography layout program. They were fabricated using photolithography and RIE etching. Several different designs are presented with some experimental results from a small-scale adaptive optics brass-board.

  10. Magellan adaptive optics first-light observations of the exoplanet β PIC b. I. Direct imaging in the far-red optical with MagAO+VisAO and in the near-IR with NICI {sup ,}

    SciTech Connect

    Males, Jared R.; Close, Laird M.; Morzinski, Katie M.; Skemer, Andrew J.; Kopon, Derek; Follette, Katherine B.; Hinz, Philip M.; Rodigas, Timothy J.; Wahhaj, Zahed; Liu, Michael C.; Nielsen, Eric L.; Chun, Mark; Puglisi, Alfio; Esposito, Simone; Riccardi, Armando; Pinna, Enrico; Xompero, Marco; Briguglio, Runa; Hayward, Thomas L. [Gemini Observatory, Southern Operations Center, c and others

    2014-05-01

    We present the first ground-based CCD (λ < 1 μm) image of an extrasolar planet. Using the Magellan Adaptive Optics system's VisAO camera, we detected the extrasolar giant planet β Pictoris b in Y-short (Y{sub S} , 0.985 μm), at a separation of 0.470 ± 0.''010 and a contrast of (1.63 ± 0.49) × 10{sup –5}. This detection has a signal-to-noise ratio of 4.1 with an empirically estimated upper limit on false alarm probability of 1.0%. We also present new photometry from the Gemini Near-Infrared Coronagraphic Imager instrument on the Gemini South telescope, in CH {sub 4S,1%} (1.58 μm), K{sub S} (2.18 μm), and K {sub cont} (2.27 μm). A thorough analysis of our photometry combined with previous measurements yields an estimated near-IR spectral type of L2.5 ± 1.5, consistent with previous estimates. We estimate log (L {sub bol}/L {sub ☉}) = –3.86 ± 0.04, which is consistent with prior estimates for β Pic b and with field early-L brown dwarfs (BDs). This yields a hot-start mass estimate of 11.9 ± 0.7 M {sub Jup} for an age of 21 ± 4 Myr, with an upper limit below the deuterium burning mass. Our L {sub bol}-based hot-start estimate for temperature is T {sub eff} = 1643 ± 32 K (not including model-dependent uncertainty). Due to the large corresponding model-derived radius of R = 1.43 ± 0.02 R {sub Jup}, this T {sub eff} is ∼250 K cooler than would be expected for a field L2.5 BD. Other young, low-gravity (large-radius), ultracool dwarfs and directly imaged EGPs also have lower effective temperatures than are implied by their spectral types. However, such objects tend to be anomalously red in the near-IR compared to field BDs. In contrast, β Pic b has near-IR colors more typical of an early-L dwarf despite its lower inferred temperature.

  11. Magellan Adaptive Optics First-light Observations of the Exoplanet β Pic B. I. Direct Imaging in the Far-red Optical with MagAO+VisAO and in the Near-ir with NICI

    NASA Astrophysics Data System (ADS)

    Males, Jared R.; Close, Laird M.; Morzinski, Katie M.; Wahhaj, Zahed; Liu, Michael C.; Skemer, Andrew J.; Kopon, Derek; Follette, Katherine B.; Puglisi, Alfio; Esposito, Simone; Riccardi, Armando; Pinna, Enrico; Xompero, Marco; Briguglio, Runa; Biller, Beth A.; Nielsen, Eric L.; Hinz, Philip M.; Rodigas, Timothy J.; Hayward, Thomas L.; Chun, Mark; Ftaclas, Christ; Toomey, Douglas W.; Wu, Ya-Lin

    2014-05-01

    We present the first ground-based CCD (λ < 1 μm) image of an extrasolar planet. Using the Magellan Adaptive Optics system's VisAO camera, we detected the extrasolar giant planet β Pictoris b in Y-short (YS , 0.985 μm), at a separation of 0.470 ± 0.''010 and a contrast of (1.63 ± 0.49) × 10-5. This detection has a signal-to-noise ratio of 4.1 with an empirically estimated upper limit on false alarm probability of 1.0%. We also present new photometry from the Gemini Near-Infrared Coronagraphic Imager instrument on the Gemini South telescope, in CH 4S,1% (1.58 μm), KS (2.18 μm), and K cont (2.27 μm). A thorough analysis of our photometry combined with previous measurements yields an estimated near-IR spectral type of L2.5 ± 1.5, consistent with previous estimates. We estimate log (L bol/L ⊙) = -3.86 ± 0.04, which is consistent with prior estimates for β Pic b and with field early-L brown dwarfs (BDs). This yields a hot-start mass estimate of 11.9 ± 0.7 M Jup for an age of 21 ± 4 Myr, with an upper limit below the deuterium burning mass. Our L bol-based hot-start estimate for temperature is T eff = 1643 ± 32 K (not including model-dependent uncertainty). Due to the large corresponding model-derived radius of R = 1.43 ± 0.02 R Jup, this T eff is ~250 K cooler than would be expected for a field L2.5 BD. Other young, low-gravity (large-radius), ultracool dwarfs and directly imaged EGPs also have lower effective temperatures than are implied by their spectral types. However, such objects tend to be anomalously red in the near-IR compared to field BDs. In contrast, β Pic b has near-IR colors more typical of an early-L dwarf despite its lower inferred temperature.

  12. Adaptive optics for space debris tracking

    NASA Astrophysics Data System (ADS)

    Bennet, Francis; D'Orgeville, Celine; Gao, Yue; Gardhouse, William; Paulin, Nicolas; Price, Ian; Rigaut, Francois; Ritchie, Ian T.; Smith, Craig H.; Uhlendorf, Kristina; Wang, Yanjie

    2014-07-01

    Space debris in Low Earth Orbit (LEO) is becoming an increasing threat to satellite and spacecraft. A reliable and cost effective method for detecting possible collisions between orbiting objects is required to prevent an exponential growth in the number of debris. Current RADAR survey technologies used to monitor the orbits of thousands of space debris objects are relied upon to manoeuvre operational satellites to prevent possible collisions. A complimentary technique, ground-based laser LIDAR (Light Detection and Ranging) have been used to track much smaller objects with higher accuracy than RADAR, giving greater prediction of possible collisions and avoiding unnecessary manoeuvring. Adaptive optics will play a key role in any ground based LIDAR tracking system as a cost effective way of utilising smaller ground stations or less powerful lasers. The use of high power and high energy lasers for the orbital modification of debris objects will also require an adaptive optic system to achieve the high photon intensity on the target required for photon momentum transfer and laser ablation. EOS Space Systems have pioneered the development of automated laser space debris tracking for objects in low Earth orbit. The Australian National University have been developing an adaptive optics system to improve this space debris tracking capability at the EOS Space Systems Mount Stromlo facility in Canberra, Australia. The system is integrated with the telescope and commissioned as an NGS AO system before moving on to LGS AO and tracking operations. A pulsed laser propagated through the telescope is used to range the target using time of flight data. Adaptive optics is used to increase the maximum range and number or targets available to the LIDAR system, by correcting the uplink laser beam. Such a system presents some unique challenges for adaptive optics: high power lasers reflecting off deformable mirrors, high slew rate tracking, and variable off-axis tracking correction. A

  13. Electron density measurements for plasma adaptive optics

    NASA Astrophysics Data System (ADS)

    Neiswander, Brian W.

    Over the past 40 years, there has been growing interest in both laser communications and directed energy weapons that operate from moving aircraft. As a laser beam propagates from an aircraft in flight, it passes through boundary layers, turbulence, and shear layers in the near-region of the aircraft. These fluid instabilities cause strong density gradients which adversely affect the transmission of laser energy to a target. Adaptive optics provides corrective measures for this problem but current technology cannot respond quickly enough to be useful for high speed flight conditions. This research investigated the use of plasma as a medium for adaptive optics for aero-optics applications. When a laser beam passes through plasma, its phase is shifted proportionally to the electron density and gas heating within the plasma. As a result, plasma can be utilized as a dynamically controllable optical medium. Experiments were carried out using a cylindrical dielectric barrier discharge plasma chamber which generated a sub-atmospheric pressure, low-temperature plasma. An electrostatic model of this design was developed and revealed an important design constraint relating to the geometry of the chamber. Optical diagnostic techniques were used to characterize the plasma discharge. Single-wavelength interferometric experiments were performed and demonstrated up to 1.5 microns of optical path difference (OPD) in a 633 nm laser beam. Dual-wavelength interferometry was used to obtain time-resolved profiles of the plasma electron density and gas heating inside the plasma chamber. Furthermore, a new multi-wavelength infrared diagnostic technique was developed and proof-of-concept simulations were conducted to demonstrate the system's capabilities.

  14. Micro-optics for imaging.

    SciTech Connect

    Boye, Robert R.

    2010-09-01

    This project investigates the fundamental imaging capability of an optic with a physical thickness substantially less than 1 mm. The analysis assumes that post-processing can overcome certain restrictions such as detector pixel size and image degradation due to aberrations. A first order optical analysis quickly reveals the limitations of even an ideal thin lens to provide sufficient image resolution and provides the justification for pursuing an annular design. Some straightforward examples clearly show the potential of this approach. The tradeoffs associated with annular designs, specifically field of view limitations and reduced mid-level spatial frequencies, are discussed and their impact on the imaging performance evaluated using several imaging examples. Additionally, issues such as detector acceptance angle and the need to balance aberrations with resolution are included in the analysis. With these restrictions, the final results present an excellent approximation of the expected performance of the lens designs presented.

  15. Hands-on demonstrations and teaching tools for optics and adaptive optics

    NASA Astrophysics Data System (ADS)

    Arion, Douglas N.; Crosby, Kevin M.; Lyons, Daniel; Rand, Kendra; Randolph, Ann

    2003-10-01

    A set of demonstrations suitable for use in classrooms at the secondary, undergraduate and graduate level, and for public use in museums and science centers were developed to exhibit basic optics principles, vision science, and adaptive optics techniques. This paper will exhibit these demonstrations, and how they can be used to promote understanding of optics principles in a wide range of applicable areas. Demonstrations include units showing image formation, orientation, and scale; a unit showing 3-dimensional ray tracing through optical systems using a scattering medium and laser diodes; a unit allowing users to directly observe the color sensitivity of their eyes; and a demonstration directly demonstrating wavefront errors, image distortion, and a Shack-Hartmann sensor for wavefront measurement. Together, these bring real-world optical principles into the hands-on regime, and demystify optics principles that are difficult to visualize in three dimensions. These demonstrations are currently in use at the Keck Observatory in Hawaii, at Nauticus in Virginia, at the Yerkes Observatory and at Carthage College in Wisconsin, as well as at several middle and high schools in Illinois and Wisconsin. They have also been integrated into a unit in the Hands on Universe program published by the Lawrence Hall of Science. This work was supported by the National Science Foundation through the Center for Adaptive Optics.

  16. MEMS-based extreme adaptive optics for planet detection

    SciTech Connect

    Macintosh, B A; Graham, J R; Oppenheimer, B; Poyneer, L; Sivaramakrishnan, A; Veran, J

    2005-11-18

    The next major step in the study of extrasolar planets will be the direct detection, resolved from their parent star, of a significant sample of Jupiter-like extrasolar giant planets. Such detection will open up new parts of the extrasolar planet distribution and allow spectroscopic characterization of the planets themselves. Detecting Jovian planets at 5-50 AU scale orbiting nearby stars requires adaptive optics systems and coronagraphs an order of magnitude more powerful than those available today--the realm of ''Extreme'' adaptive optics. We present the basic requirements and design for such a system, the Gemini Planet Imager (GPI.) GPI will require a MEMS-based deformable mirror with good surface quality, 2-4 micron stroke (operated in tandem with a conventional low-order ''woofer'' mirror), and a fully-functional 48-actuator-diameter aperture.

  17. Neptune and Titan Observed with Keck Telescope Adaptive Optics

    SciTech Connect

    Max, C.E.; Macintosh, B.A.; Gibbard, S.; Gavel, D.T.; Roe, H.; De Pater, I.; Ghez, A.M.; Acton, S.; Wizinowich, P.L.; Lai, O.

    2000-05-05

    The authors report on observations taken during engineering science validation time using the new adaptive optics system at the 10-m Keck II Telescope. They observe Neptune and Titan at near-infrared wavelengths. These objects are ideal for adaptive optics imaging because they are bright and small, yet have many diffraction-limited resolution elements across their disks. In addition Neptune and Titan have prominent physical features, some of which change markedly with time. They have observed infrared-bright storms on Neptune, and very low-albedo surface regions on Titan, Saturn's largest moon, Spatial resolution on Neptune and Titan was 0.05-0.06 and 0.04-0.05 arc sec, respectively.

  18. The CHARA Array Adaptive Optics Program

    NASA Astrophysics Data System (ADS)

    Ten Brummelaar, Theo; Che, Xiao; McAlister, Harold A.; Ireland, Michael; Monnier, John D.; Mourard, Denis; Ridgway, Stephen T.; sturmann, judit; Sturmann, Laszlo; Turner, Nils H.; Tuthill, Peter

    2016-01-01

    The CHARA array is an optical/near infrared interferometer consisting of six 1-meter diameter telescopes the longest baseline of which is 331 meters. With sub-millisecond angular resolution, the CHARA array is able to spatially resolve nearby stellar systems to reveal the detailed structures. To improve the sensitivity and scientific throughput, the CHARA array was funded by NSF-ATI in 2011, and by NSF-MRI in 2015, for an upgrade of adaptive optics (AO) systems to all six telescopes. The initial grant covers Phase I of the adaptive optics system, which includes an on-telescope Wavefront Sensor and non-common-path (NCP) error correction. The WFS use a fairly standard Shack-Hartman design and will initially close the tip tilt servo and log wavefront errors for use in data reduction and calibration. The second grant provides the funding for deformable mirrors for each telescope which will be used closed loop to remove atmospheric aberrations from the beams. There are then over twenty reflections after the WFS at the telescopes that bring the light several hundred meters into the beam combining laboratory. Some of these, including the delay line and beam reducing optics, are powered elements, and some of them, in particular the delay lines and telescope Coude optics, are continually moving. This means that the NCP problems in an interferometer are much greater than those found in more standard telescope systems. A second, slow AO system is required in the laboratory to correct for these NCP errors. We will breifly describe the AO system, and it's current status, as well as discuss the new science enabled by the system with a focus on our YSO program.

  19. Performance evaluation of a sensorless adaptive optics multiphoton microscope.

    PubMed

    Skorsetz, Martin; Artal, Pablo; Bueno, Juan M

    2016-03-01

    A wavefront sensorless adaptive optics technique was combined with a custom-made multiphoton microscope to correct for specimen-induced aberrations. A liquid-crystal-on-silicon (LCoS) modulator was used to systematically generate Zernike modes during image recording. The performance of the instrument was evaluated in samples providing different nonlinear signals and the benefit of correcting higher order aberrations was always noticeable (in both contrast and resolution). The optimum aberration pattern was stable in time for the samples here involved. For a particular depth location within the sample, the wavefront to be precompensated was independent on the size of the imaged area (up to ∼ 360 × 360 μm(2)). The mode combination optimizing the recorded image depended on the Zernike correction control sequence; however, the final images hardly differed. At deeper locations, a noticeable dominance of spherical aberration was found. The influence of other aberration terms was also compared to the effect of the spherical aberration.

  20. The ESO Adaptive Optics Facility under Test

    NASA Astrophysics Data System (ADS)

    Arsenault, Robin; Madec, Pierre-Yves; Paufique, Jerome; La Penna, Paolo; Stroebele, Stefan; Vernet, Elise; Pirard, Jean-François; Hackenberg, Wolfgang; Kuntschner, Harald; Kolb, Johann; Muller, Nicolas; Le Louarn, Miska; Amico, Paola; Hubin, Norbert; Lizon, Jean-Louis; Ridings, Rob; Abad, Jose; Fischer, Gert; Heinz, Volker; Kiekebusch, Mario; Argomedo, Javier; Conzelmann, Ralf; Tordo, Sebastien; Donaldson, Rob; Soenke, Christian; Duhoux, Philippe; Fedrigo, Enrico; Delabre, Bernard; Jost, Andrea; Duchateau, Michel; Downing, Mark; Moreno, Javier; Manescau, Antonio; Bonaccini Calia, Domenico; Quattri, Marco; Dupuy, Christophe; Guidolin, Ivan; Comin, Mauro; Guzman, Ronald; Buzzoni, Bernard; Quentin, Jutta; Lewis, Steffan; Jolley, Paul; Kraus, Max; Pfrommer, Thomas; Garcia-Rissmann, Aurea; Biasi, Roberto; Gallieni, Daniele; Stuik, Remko

    2013-12-01

    The Adaptive Optics Facility project has received most of its subsystems in Garching and the ESO Integration Hall has become the central operation location for the next phase of the project. The main test bench ASSIST and the 2nd Generation M2-Unit (hosting the Deformable Secondary Mirror) have been granted acceptance late 2012. The DSM will now undergo a series of tests on ASSIST to qualify its optical performance which launches the System Test Phase of the AOF. The tests will validate the AO modules operation with the DSM: first the GRAAL adaptive optics module for Hawk-I in natural guide star AO mode on-axis and then its Ground Layer AO mode. This will be followed by the GALACSI (for MUSE) Wide-Field-Mode (GLAO) and then the more challenging Narrow-Field-Mode (LTAO). We will report on the status of the subsystems at the time of the conference but also on the performance of the delivered ASSIST test bench, the DSM and the 20 Watt Sodium fiber Laser pre-production unit which has validated all specifications before final manufacturing of the serial units. We will also present some considerations and tools to ensure an efficient operation of the Facility in Paranal.

  1. Optically detected magnetic resonance imaging

    SciTech Connect

    Blank, Aharon; Shapiro, Guy; Fischer, Ran; London, Paz; Gershoni, David

    2015-01-19

    Optically detected magnetic resonance provides ultrasensitive means to detect and image a small number of electron and nuclear spins, down to the single spin level with nanoscale resolution. Despite the significant recent progress in this field, it has never been combined with the power of pulsed magnetic resonance imaging techniques. Here, we demonstrate how these two methodologies can be integrated using short pulsed magnetic field gradients to spatially encode the sample. This result in what we denote as an 'optically detected magnetic resonance imaging' technique. It offers the advantage that the image is acquired in parallel from all parts of the sample, with well-defined three-dimensional point-spread function, and without any loss of spectroscopic information. In addition, this approach may be used in the future for parallel but yet spatially selective efficient addressing and manipulation of the spins in the sample. Such capabilities are of fundamental importance in the field of quantum spin-based devices and sensors.

  2. Guide star lasers for adaptive optics

    NASA Astrophysics Data System (ADS)

    Roberts, William Thomas, Jr.

    Exploitation of the imaging capabilities of the new generation of ground-based astronomical telescopes relies heavily on Adaptive Optics (AO). Current AO system designs call for sodium guide star lasers capable of producing at least eight Watts of power tuned to the peak of the sodium D2 line, with a high duty cycle to avoid saturation, and with 0.5-1.0 GHz spectral broadening. This work comprises development and testing of six candidate laser systems and materials which may afford a path to achieving these goals. An end-pumped CW dye laser producing 4.0 Watts of tuned output power was developed and used to obtain the first accurate measurement of sodium layer scattering efficiency. Methods of optimizing the laser output through improving pump overlap efficiency and reducing the number of intracavity scattering surfaces are covered. The 1181 nm fluorescence peak of Mn5+ ion in Ba5 (PO4)3Cl could be tuned and doubled to reach 589 nm. While efforts to grow this crystal were under way, the Mn5+ ion in natural apatite (Ca5(PO4)3F) was studied as a potential laser material. Fluorescence saturation measurements and transmission saturation are presented, as well as efforts to obtain CW lasing in natural apatite. A Q-switched laser color-center laser in LiF : F-2 was developed and successfully tuned and doubled to the sodium D 2 line. Broad-band lasing of 80 mW and tuned narrow-band lasing of 35 mW at 1178 nm were obtained with 275 mW of input pump power at 1064 nm. The measured thermal properties of this material indicate its potential for scaling to much higher power. A Q-switched intracavity Raman laser was developed in which CaWO 4 was used to shift a Nd:YAG laser, the frequency-doubled output of which was centered at 589.3 nm. To obtain light at 589.0 nm, a compositionally tuned pump laser of Nd : Y3Ga1.1Al3.9O 12 was produced which generated the desired shift, but was inhomogeneous broadened, limiting the tunable power of the material. Finally, temperature tuning of

  3. Non-iterative adaptive optical microscopy using wavefront sensing

    NASA Astrophysics Data System (ADS)

    Tao, X.; Azucena, O.; Kubby, J.

    2016-03-01

    This paper will review the development of wide-field and confocal microscopes with wavefront sensing and adaptive optics for correcting refractive aberrations and compensating scattering when imaging through thick tissues (Drosophila embryos and mouse brain tissue). To make wavefront measurements in biological specimens we have modified the laser guide-star techniques used in astronomy for measuring wavefront aberrations that occur as star light passes through Earth's turbulent atmosphere. Here sodium atoms in Earth's mesosphere, at an altitude of 95 km, are excited to fluoresce at resonance by a high-power sodium laser. The fluorescent light creates a guide-star reference beacon at the top of the atmosphere that can be used for measuring wavefront aberrations that occur as the light passes through the atmosphere. We have developed a related approach for making wavefront measurements in biological specimens using cellular structures labeled with fluorescent proteins as laser guide-stars. An example is a fluorescently labeled centrosome in a fruit fly embryo or neurons and dendrites in mouse brains. Using adaptive optical microscopy we show that the Strehl ratio, the ratio of the peak intensity of an aberrated point source relative to the diffraction limited image, can be improved by an order of magnitude when imaging deeply into live dynamic specimens, enabling near diffraction limited deep tissue imaging.

  4. Programmable Iterative Optical Image And Data Processing

    NASA Technical Reports Server (NTRS)

    Jackson, Deborah J.

    1995-01-01

    Proposed method of iterative optical image and data processing overcomes limitations imposed by loss of optical power after repeated passes through many optical elements - especially, beam splitters. Involves selective, timed combination of optical wavefront phase conjugation and amplification to regenerate images in real time to compensate for losses in optical iteration loops; timing such that amplification turned on to regenerate desired image, then turned off so as not to regenerate other, undesired images or spurious light propagating through loops from unwanted reflections.

  5. Anisoplanatism in adaptive optics systems due to pupil aberrations

    SciTech Connect

    Bauman, B

    2005-08-01

    Adaptive optics systems typically include an optical relay that simultaneously images the science field to be corrected and also a set of pupil planes conjugate to the deformable mirror of the system. Often, in the optical spaces where DM's are placed, the pupils are aberrated, leading to a displacement and/or distortion of the pupil that varies according to field position--producing a type of anisoplanatism, i.e., a degradation of the AO correction with field angle. The pupil aberration phenomenon is described and expressed in terms of Seidel aberrations. An expression for anisoplanatism as a function of pupil distortion is derived, an example of an off-axis parabola is given, and a convenient method for controlling pupil-aberration-generated anisoplanatism is proposed.

  6. Luminescent probes for optical in vivo imaging

    NASA Astrophysics Data System (ADS)

    Texier, Isabelle; Josserand, Veronique; Garanger, Elisabeth; Razkin, Jesus; Jin, Zhaohui; Dumy, Pascal; Favrot, Marie; Boturyn, Didier; Coll, Jean-Luc

    2005-04-01

    Going along with instrumental development for small animal fluorescence in vivo imaging, we are developing molecular fluorescent probes, especially for tumor targeting. Several criteria have to be taken into account for the optimization of the luminescent label. It should be adapted to the in vivo imaging optical conditions : red-shifted absorption and emission, limited overlap between absorption and emission for a good signal filtering, optimized luminescence quantum yield, limited photo-bleaching. Moreover, the whole probe should fulfill the biological requirements for in vivo labeling : adapted blood-time circulation, biological conditions compatibility, low toxicity. We here demonstrate the ability of the imaging fluorescence set-up developed in LETI to image the bio-distribution of molecular probes on short times after injection. Targeting with Cy5 labeled holo-transferrin of subcutaneous TS/Apc (angiogenic murine breast carcinoma model) or IGROV1 (human ovarian cancer) tumors was achieved. Differences in the kinetics of the protein uptake by the tumors were evidenced. IGROV1 internal metastatic nodes implanted in the peritoneal cavity could be detected in nude mice. However, targeted metastatic nodes in lung cancer could only be imaged after dissection of the mouse. These results validate our fluorescence imaging set-up and the use of Cy5 as a luminescent label. New fluorescent probes based on this dye and a molecular delivery template (the RAFT molecule) can thus be envisioned.

  7. Design considerations for CELT adaptive optics

    NASA Astrophysics Data System (ADS)

    Dekany, Richard G.; Nelson, Jerry E.; Bauman, Brian J.

    2000-07-01

    California Institute of Technology and University of California have begun conceptual design studies for a new telescope for astronomical research at visible and infrared wavelengths. The California Extremely Large Telescope (CELT) is currently envisioned as a filled-aperture, steerable, segmented telescope of approximately 30 m diameter. The key to satisfying many of the science goals of this observatory is the availability of diffraction-limited wavefront control. We describe potential observing modes of CELT, including a discussion of the several major outstanding AO system architectural design issues to be resolved prior to the initiation of the detailed design of the adaptive optics capability.

  8. Adaptive Holographic Fiber-Optic Interferometer

    NASA Astrophysics Data System (ADS)

    Kozhevnikov, Nikolai M.; Lipovskaya, Margarita J.

    1990-04-01

    Interaction of phase-modulated light beams in photorefractive local inertial responce media was analysed. Interaction of this type allows to registrate phase-modulated signals adaptively under low frequency phase disturbtion. The experiments on multimode fiber-optic interferometer with demodulation element based on photorefractive bacteriorhodopsin-doped polimer film are described. As the writing of dynamic phase hologram is an inertial process the signal fluctuations with the frequencies up to 100 Hz can be canceled. The hologram efficiencies are enough to registrate high frequency phase shifts ~10-4 radn.

  9. Hadamard multimode optical imaging transceiver

    DOEpatents

    Cooke, Bradly J; Guenther, David C; Tiee, Joe J; Kellum, Mervyn J; Olivas, Nicholas L; Weisse-Bernstein, Nina R; Judd, Stephen L; Braun, Thomas R

    2012-10-30

    Disclosed is a method and system for simultaneously acquiring and producing results for multiple image modes using a common sensor without optical filtering, scanning, or other moving parts. The system and method utilize the Walsh-Hadamard correlation detection process (e.g., functions/matrix) to provide an all-binary structure that permits seamless bridging between analog and digital domains. An embodiment may capture an incoming optical signal at an optical aperture, convert the optical signal to an electrical signal, pass the electrical signal through a Low-Noise Amplifier (LNA) to create an LNA signal, pass the LNA signal through one or more correlators where each correlator has a corresponding Walsh-Hadamard (WH) binary basis function, calculate a correlation output coefficient for each correlator as a function of the corresponding WH binary basis function in accordance with Walsh-Hadamard mathematical principles, digitize each of the correlation output coefficient by passing each correlation output coefficient through an Analog-to-Digital Converter (ADC), and performing image mode processing on the digitized correlation output coefficients as desired to produce one or more image modes. Some, but not all, potential image modes include: multi-channel access, temporal, range, three-dimensional, and synthetic aperture.

  10. In vivo cellular visualization of the human retina using optical coherence tomography and adaptive optics

    SciTech Connect

    Olivier, S S; Jones, S M; Chen, D C; Zawadzki, R J; Choi, S S; Laut, S P; Werner, J S

    2006-01-05

    Optical coherence tomography (OCT) sees the human retina sharply with adaptive optics. In vivo cellular visualization of the human retina at micrometer-scale resolution is possible by enhancing Fourier-domain optical-coherence tomography with adaptive optics, which compensate for the eye's optical aberrations.

  11. Miniature hybrid optical imaging lens

    DOEpatents

    Sitter, D.N. Jr.; Simpson, M.L.

    1997-10-21

    A miniature lens system that corrects for imaging and chromatic aberrations is disclosed, the lens system being fabricated from primarily commercially-available components. A first element at the input to a lens housing is an aperture stop. A second optical element is a refractive element with a diffractive element closely coupled to, or formed a part of, the rear surface of the refractive element. Spaced closely to the diffractive element is a baffle to limit the area of the image, and this is closely followed by a second refractive lens element to provide the final correction. The image, corrected for aberrations exits the last lens element to impinge upon a detector plane were is positioned any desired detector array. The diffractive element is fabricated according to an equation that includes, as variables, the design wavelength, the index of refraction and the radius from an optical axis of the lens system components. 2 figs.

  12. Miniature hybrid optical imaging lens

    DOEpatents

    Sitter, Jr., David N.; Simpson, Marc L.

    1997-01-01

    A miniature lens system that corrects for imaging and chromatic aberrations, the lens system being fabricated from primarily commercially-available components. A first element at the input to a lens housing is an aperture stop. A second optical element is a refractive element with a diffractive element closely coupled to, or formed a part of, the rear surface of the refractive element. Spaced closely to the diffractive element is a baffle to limit the area of the image, and this is closely followed by a second refractive lens element to provide the final correction. The image, corrected for aberrations exits the last lens element to impinge upon a detector plane were is positioned any desired detector array. The diffractive element is fabricated according to an equation that includes, as variables, the design wavelength, the index of refraction and the radius from an optical axis of the lens system components.

  13. Optical Analysis of Microscope Images

    NASA Astrophysics Data System (ADS)

    Biles, Jonathan R.

    Microscope images were analyzed with coherent and incoherent light using analog optical techniques. These techniques were found to be useful for analyzing large numbers of nonsymbolic, statistical microscope images. In the first part phase coherent transparencies having 20-100 human multiple myeloma nuclei were simultaneously photographed at 100 power magnification using high resolution holographic film developed to high contrast. An optical transform was obtained by focussing the laser onto each nuclear image and allowing the diffracted light to propagate onto a one dimensional photosensor array. This method reduced the data to the position of the first two intensity minima and the intensity of successive maxima. These values were utilized to estimate the four most important cancer detection clues of nuclear size, shape, darkness, and chromatin texture. In the second part, the geometric and holographic methods of phase incoherent optical processing were investigated for pattern recognition of real-time, diffuse microscope images. The theory and implementation of these processors was discussed in view of their mutual problems of dimness, image bias, and detector resolution. The dimness problem was solved by either using a holographic correlator or a speckle free laser microscope. The latter was built using a spinning tilted mirror which caused the speckle to change so quickly that it averaged out during the exposure. To solve the bias problem low image bias templates were generated by four techniques: microphotography of samples, creation of typical shapes by computer graphics editor, transmission holography of photoplates of samples, and by spatially coherent color image bias removal. The first of these templates was used to perform correlations with bacteria images. The aperture bias was successfully removed from the correlation with a video frame subtractor. To overcome the limited detector resolution it is necessary to discover some analog nonlinear intensity

  14. Adaptive predictive image coding using local characteristics

    NASA Astrophysics Data System (ADS)

    Hsieh, C. H.; Lu, P. C.; Liou, W. G.

    1989-12-01

    The paper presents an efficient adaptive predictive coding method using the local characteristics of images. In this method, three coding schemes, namely, mean, subsampling combined with fixed DPCM, and ADPCM/PCM, are used and one of these is chosen adaptively based on the local characteristics of images. The prediction parameters of the two-dimensional linear predictor in the ADPCM/PCM are extracted on a block by block basis. Simulation results show that the proposed method is effective in reducing the slope overload distortion and the granular noise at low bit rates, and thus it can improve the visual quality of reconstructed images.

  15. Image Resolution in Optical Nanoscopy

    PubMed Central

    Nieuwenhuizen, Robert P.J.; Lidke, Keith A.; Bates, Mark; Puig, Daniela Leyton; Grünwald, David; Stallinga, Sjoerd; Rieger, Bernd

    2014-01-01

    Resolution in optical nanoscopy depends on the localization uncertainty of single fluorescent labels, the density of labels covering the sample, and the sample’s spatial structure. Currently there is no integral, practical resolution measure that takes all factors into account. Here we introduce such a measure that can be computed directly from the image. We demonstrate its validity and benefits on 2D and 3D localization microscopy images of tubulin and actin filaments. Our approach makes it possible to compare achieved resolutions in images taken with different nanoscopy methods, optimize and rank different emitter localization and labeling strategies, define a stopping criterion for data acquisition, describe image anisotropy and heterogeneity, and, surprisingly, estimate the average number of localizations per emitter. Our findings challenge the current focus on obtaining the best localization precision, but instead show how the best image resolution can be achieved as fast as possible. PMID:23624665

  16. Speckle reduction in optical coherence tomography by adaptive total variation method

    NASA Astrophysics Data System (ADS)

    Wu, Tong; Shi, Yaoyao; Liu, Youwen; He, Chongjun

    2015-12-01

    An adaptive total variation method based on the combination of speckle statistics and total variation restoration is proposed and developed for reducing speckle noise in optical coherence tomography (OCT) images. The statistical distribution of the speckle noise in OCT image is investigated and measured. With the measured parameters such as the mean value and variance of the speckle noise, the OCT image is restored by the adaptive total variation restoration method. The adaptive total variation restoration algorithm was applied to the OCT images of a volunteer's hand skin, which showed effective speckle noise reduction and image quality improvement. For image quality comparison, the commonly used median filtering method was also applied to the same images to reduce the speckle noise. The measured results demonstrate the superior performance of the adaptive total variation restoration method in terms of image signal-to-noise ratio, equivalent number of looks, contrast-to-noise ratio, and mean square error.

  17. Large aperture adaptive optics for intense lasers

    NASA Astrophysics Data System (ADS)

    Deneuville, François; Ropert, Laurent; Sauvageot, Paul; Theis, Sébastien

    2015-05-01

    ISP SYSTEM has developed a range of large aperture electro-mechanical deformable mirrors (DM) suitable for ultra short pulsed intense lasers. The design of the MD-AME deformable mirror is based on force application on numerous locations thanks to electromechanical actuators driven by stepper motors. DM design and assembly method have been adapted to large aperture beams and the performances were evaluated on a first application for a beam with a diameter of 250mm at 45° angle of incidence. A Strehl ratio above 0.9 was reached for this application. Simulations were correlated with measurements on optical bench and the design has been validated by calculation for very large aperture (up to Ø550mm). Optical aberrations up to Zernike order 5 can be corrected with a very low residual error as for actual MD-AME mirror. Amplitude can reach up to several hundreds of μm for low order corrections. Hysteresis is lower than 0.1% and linearity better than 99%. Contrary to piezo-electric actuators, the μ-AME actuators avoid print-through effects and they permit to keep the mirror shape stable even unpowered, providing a high resistance to electro-magnetic pulses. The MD-AME mirrors can be adapted to circular, square or elliptical beams and they are compatible with all dielectric or metallic coatings.

  18. Kalman filter based control for Adaptive Optics

    NASA Astrophysics Data System (ADS)

    Petit, Cyril; Quiros-Pacheco, Fernando; Conan, Jean-Marc; Kulcsár, Caroline; Raynaud, Henri-François; Fusco, Thierry

    2004-12-01

    Classical Adaptive Optics suffer from a limitation of the corrected Field Of View. This drawback has lead to the development of MultiConjugated Adaptive Optics. While the first MCAO experimental set-ups are presently under construction, little attention has been paid to the control loop. This is however a key element in the optimization process especially for MCAO systems. Different approaches have been proposed in recent articles for astronomical applications : simple integrator, Optimized Modal Gain Integrator and Kalman filtering. We study here Kalman filtering which seems a very promising solution. Following the work of Brice Leroux, we focus on a frequential characterization of kalman filters, computing a transfer matrix. The result brings much information about their behaviour and allows comparisons with classical controllers. It also appears that straightforward improvements of the system models can lead to static aberrations and vibrations filtering. Simulation results are proposed and analysed thanks to our frequential characterization. Related problems such as model errors, aliasing effect reduction or experimental implementation and testing of Kalman filter control loop on a simplified MCAO experimental set-up could be then discussed.

  19. The Giant Magellan Telescope adaptive optics program

    NASA Astrophysics Data System (ADS)

    Bouchez, Antonin H.; Acton, D. Scott; Agapito, Guido; Arcidiacono, Carmelo; Bennet, Francis; Biliotti, Valdemaro; Bonaglia, Marco; Briguglio, Runa; Brusa-Zappellini, Guido; Busoni, Lorenzo; Carbonaro, Luca; Codona, Johanan L.; Conan, Rodolphe; Connors, Thomas; Durney, Oliver; Espeland, Brady; Esposito, Simone; Fini, Luca; Gardhouse, Rusty; Gauron, Thomas M.; Hart, Michael; Hinz, Philip M.; Kanneganti, Srikrishna; Kibblewhite, Edward J.; Knox, Russell P.; McLeod, Brian A.; McMahon, Thomas; Montoya, Manny; Norton, Timothy J.; Ordway, Mark P.; d'Orgeville, Celine; Parcell, Simon; Piatrou, Piotr K.; Pinna, Enrico; Price, Ian; Puglisi, Alfio; Quiros-Pacheco, Fernando; Riccardi, Armando; Roll, John B.; Trancho, Gelys; Uhlendorf, Kristina; Vaitheeswaran, Vidhya; van Dam, Marcos A.; Weaver, David; Xompero, Marco

    2012-07-01

    The Giant Magellan Telescope adaptive optics system will be an integral part of the telescope, providing laser guide star generation, wavefront sensing, and wavefront correction to most of the currently envisioned instruments. The system will provide three observing modes: Natural Guidestar AO (NGSAO), Laser Tomography AO (LTAO), and Ground Layer AO (GLAO). Every AO observing mode will use the telescope’s segmented adaptive secondary mirror to deliver a corrected beam directly to the instruments. High-order wavefront sensing for the NGSAO and LTAO modes is provided by a set of wavefront sensors replicated for each instrument and fed by visible light reflected off the cryostat window. An infrared natural guidestar wavefront sensor with open-loop AO correction is also required to sense tip-tilt, focus, segment piston, and dynamic calibration errors in the LTAO mode. GLAO mode wavefront sensing is provided by laser guidestars over a ~5 arcminute field of view, and natural guidestars over wider fields. A laser guidestar facility will project 120 W of 589 nm laser light in 6 beacons from the periphery of the primary mirror. An off-axis phasing camera and primary and secondary mirror metrology systems will ensure that the telescope optics remain phased. We describe the system requirements, overall architecture, and innovative solutions found to the challenges presented by high-order AO on a segmented extremely large telescope. Further details may be found in specific papers on each of the observing modes and major subsystems.

  20. Dual electro-optical modulator polarimeter based on adaptive optics scanning laser ophthalmoscope.

    PubMed

    Song, Hongxin; Qi, Xiaofeng; Zou, Weiyao; Zhong, Zhangyi; Burns, Stephen A

    2010-10-11

    We constructed a high speed and high-resolution Stokes vector retinal imaging polarimeter with dual electro-optical modulators based on adaptive optics scanning laser ophthalmoscope. By varying the voltages on the EO crystals line by line, we were able to measure over 500,000 Stokes vectors per second. We used this system in three human subjects demonstrating the capability of the system to be employed in vivo. The high speed effectively decreases the adverse impact of eye motion induced errors in polarization calculations, improving the contrast of retinal structures based on their polarization properties. PMID:20941089

  1. Performance of the Keck Observatory adaptive optics system

    SciTech Connect

    van Dam, M A; Mignant, D L; Macintosh, B A

    2004-01-19

    In this paper, the adaptive optics (AO) system at the W.M. Keck Observatory is characterized. The authors calculate the error budget of the Keck AO system operating in natural guide star mode with a near infrared imaging camera. By modeling the control loops and recording residual centroids, the measurement noise and band-width errors are obtained. The error budget is consistent with the images obtained. Results of sky performance tests are presented: the AO system is shown to deliver images with average Strehl ratios of up to 0.37 at 1.58 {micro}m using a bright guide star and 0.19 for a magnitude 12 star.

  2. Optical design of the adaptive optics laser guide star system

    SciTech Connect

    Bissinger, H.

    1994-11-15

    The design of an adaptive optics package for the 3 meter Lick telescope is presented. This instrument package includes a 69 actuator deformable mirror and a Hartmann type wavefront sensor operating in the visible wavelength; a quadrant detector for the tip-tile sensor and a tip-tilt mirror to stabilize atmospheric first order tip-tile errors. A high speed computer drives the deformable mirror to achieve near diffraction limited imagery. The different optical components and their individual design constraints are described. motorized stages and diagnostics tools are used to operate and maintain alignment throughout observation time from a remote control room. The expected performance are summarized and actual results of astronomical sources are presented.

  3. Performance predictions for the Keck telescope adaptive optics system

    SciTech Connect

    Gavel, D.T.; Olivier, S.S.

    1995-08-07

    The second Keck ten meter telescope (Keck-11) is slated to have an infrared-optimized adaptive optics system in the 1997--1998 time frame. This system will provide diffraction-limited images in the 1--3 micron region and the ability to use a diffraction-limited spectroscopy slit. The AO system is currently in the preliminary design phase and considerable analysis has been performed in order to predict its performance under various seeing conditions. In particular we have investigated the point-spread function, energy through a spectroscopy slit, crowded field contrast, object limiting magnitude, field of view, and sky coverage with natural and laser guide stars.

  4. Manufacturing of the ESO adaptive optics facility

    NASA Astrophysics Data System (ADS)

    Arsenault, R.,; Madec, P.-Y.; Hubin, N.; Stroebele, S.; Paufique, J.; Vernet, E.; Hackenberg, W.; Pirard, J.-F.; Jochum, L.; Glindemann, A.; Jost, A.; Conzelmann, R.; Kiekebusch, M.; Tordo, S.; Lizon, J.-L.; Donaldson, R.; Fedrigo, E.; Soenke, C.; Duchateau, M.; Bruton, A.; Delabre, B.; Downing, M.; Reyes, J.; Kolb, J.; Bechet, C.; Lelouarn, M.; Bonaccini Calia, D.; Quattri, M.; Guidolin, I.; Buzzoni, B.; Dupuy, C.; Guzman, R.; Comin, M.; Silber, A.; Quentin, J.; La Penna, P.; Manescau, A.; Jolley, P.; Heinz, V.; Duhoux, P.; Argomedo, J.; Gallieni, D.; Lazzarini, P.; Biasi, R.; Andrighettoni, M.; Angerer, G.; Pescoller, D.; Stuik, R.,; Deep, A.

    2010-07-01

    The ESO Adaptive Optics Facility (AOF) consists in an evolution of one of the ESO VLT unit telescopes to a laser driven adaptive telescope with a deformable mirror in its optical train, in this case the secondary 1.1m mirror, and four Laser Guide Stars (LGSs). This evolution implements many challenging technologies like the Deformable Secondary Mirror (DSM) including a thin shell mirror (1.1 m diameter and 2mm thin), the high power Na lasers (20W), the low Read-Out Noise (RON) WaveFront Sensor (WFS) camera (< 1e-) and SPARTA the new generation of Real Time Computers (RTC) for adaptive control. It also faces many problematic similar to any Extremely Large Telescope (ELT) and as such, will validate many technologies and solutions needed for the European ELT (E-ELT) 42m telescope. The AOF will offer a very large (7 arcmin) Field Of View (FOV) GLAO correction in J, H and K bands (GRAAL+Hawk-I), a visible integral field spectrograph with a 1 arcmin GLAO corrected FOV (GALACSI-MUSE WFM) and finally a LTAO 7.5" FOV (GALACSI-MUSE NFM). Most systems of the AOF have completed final design and are in manufacturing phase. Specific activities are linked to the modification of the 8m telescope in order to accommodate the new DSM and the 4 LGS Units assembled on its Center-Piece. A one year test period in Europe is planned to test and validate all modes and their performance followed by a commissioning phase in Paranal scheduled for 2014.

  5. Daytime adaptive optics for deep space optical communications

    NASA Technical Reports Server (NTRS)

    Wilson, Keith; Troy, M.; Srinivasan, M.; Platt, B.; Vilnrotter, V.; Wright, M.; Garkanian, V.; Hemmati, H.

    2003-01-01

    The deep space optical communications subsystem offers a higher bandwidth communications link in smaller size, lower mass, and lower power consumption subsystem than does RF. To demonstrate the benefit of this technology to deep space communications NASA plans to launch an optical telecommunications package on the 2009 Mars Telecommunications orbiter spacecraft. Current performance goals are 30-Mbps from opposition, and 1-Mbps near conjunction (-3 degrees Sun-Earth-Probe angle). Yet, near conjunction the background noise from the day sky will degrade the performance of the optical link. Spectral and spatial filtering and higher modulation formats can mitigate the effects of background sky. Narrowband spectral filters can result in loss of link margin, and higher modulation formats require higher transmitted peak powers. In contrast, spatial filtering at the receiver has the potential of being lossless while providing the required sky background rejection. Adaptive optics techniques can correct wave front aberrations caused by atmospheric turbulence and enable near-diffraction-limited performance of the receiving telescope. Such performance facilitates spatial filtering, and allows the receiver field-of-view and hence the noise from the sky background to be reduced.

  6. New multiband IR imaging optics

    NASA Astrophysics Data System (ADS)

    Bayya, Shyam; Sanghera, Jasbinder; Kim, Woohong; Gibson, Daniel; Fleet, Erin; Shaw, Brandon; Hunt, Michael; Aggarwal, Ishwar

    2013-06-01

    We report new multispectral materials that transmit from 0.9 to < 12 µm in wavelength. These materials fill up the glass map for multispectral optics and vary in refractive index from 2.38 to 3.17. They show a large spread in dispersion (Abbe number) and offer some unique solutions for multispectral optics designs. One of the glasses developed is a very good candidate to replace Ge, as it has a combination of excellent properties, including high Abbe number in the LWIR, high index of 3.2, 60% lower dn/dT, and better thermal stability at working temperatures. Our results also provide a wider selection of optical materials to enable simpler achromat designs. For example, we have developed other glasses that have relatively high Abbe number in both the MWIR and LWIR regions, while our MILTRAN ceramic has low Abbe number in both regions. This makes for a very good combination of glasses and MILTRAN ceramic (analogous to crown and flint glasses in the visible) for MWIR + LWIR dual band imaging. We have designed preliminary optics for one such imager with f/2.5, 51 mm focal length and 22 degrees FOV using a spaced doublet of NRL's glass and MILTRAN ceramic. NRL's approach reduces the number of elements, weight, complexity and cost compared with the approach using traditional optics. Another important advantage of using NRL glasses in optics design is their negative or very low positive dn/dT, that makes it easier to athermalize the optical system.

  7. Computational adaptive optics for broadband interferometric tomography of tissues and cells

    NASA Astrophysics Data System (ADS)

    Adie, Steven G.; Mulligan, Jeffrey A.

    2016-03-01

    Adaptive optics (AO) can shape aberrated optical wavefronts to physically restore the constructive interference needed for high-resolution imaging. With access to the complex optical field, however, many functions of optical hardware can be achieved computationally, including focusing and the compensation of optical aberrations to restore the constructive interference required for diffraction-limited imaging performance. Holography, which employs interferometric detection of the complex optical field, was developed based on this connection between hardware and computational image formation, although this link has only recently been exploited for 3D tomographic imaging in scattering biological tissues. This talk will present the underlying imaging science behind computational image formation with optical coherence tomography (OCT) -- a beam-scanned version of broadband digital holography. Analogous to hardware AO (HAO), we demonstrate computational adaptive optics (CAO) and optimization of the computed pupil correction in 'sensorless mode' (Zernike polynomial corrections with feedback from image metrics) or with the use of 'guide-stars' in the sample. We discuss the concept of an 'isotomic volume' as the volumetric extension of the 'isoplanatic patch' introduced in astronomical AO. Recent CAO results and ongoing work is highlighted to point to the potential biomedical impact of computed broadband interferometric tomography. We also discuss the advantages and disadvantages of HAO vs. CAO for the effective shaping of optical wavefronts, and highlight opportunities for hybrid approaches that synergistically combine the unique advantages of hardware and computational methods for rapid volumetric tomography with cellular resolution.

  8. Optical molecular imaging in PDT

    NASA Astrophysics Data System (ADS)

    Mitra, Soumya; Snyder, John W.; Foster, Thomas H.

    2007-02-01

    Motivated by recent successes in fluorescence imaging of whole mount tissue preparations and by rapid progress in the fields of molecular imaging and molecular biology, we are exploring a number of applications of optical fluorescence imaging in superficial murine tumor models in vivo. Imaging the PDT-induced expression of the heat shock protein 70 (HSP70) in cells and in vivo is accomplished using stably transfected EMT6 cells in which the gene for GFP is under the control of the HSP70 promoter. These cells readily form solid tumors in BALB/c mice, enabling the direct imaging of the extent and time course of the activation of this promoter, with each mouse serving as its own control. Imaging of similarly transfected EMT6 cells with a HIF-1α/GFP fusion protein vector enables visualization of HIF-1α translocation to the nucleus. Recently, we have accomplished fluorescent labeling of surface antigens in vivo using intratumor and intravenous injection of fluorophore-conjugated antibodies. Injection of deep-red fluorophore-conjugated-anti-CD31 enables confocal fluorescence imaging of the tumor vasculature to depths of at least 100 microns. With the vessels rendered fluorescent in this way, a number of interesting studies become possible in the living mouse, including the direct visualization of photosensitizer distribution from perfused vessels. Using the appropriate fluorophore-conjugated antibodies, we have also been able to image infiltrating granulocytes in EMT6 tumors in response to PDT in vivo.

  9. Pioneer imaging photopolarimeter optical system.

    NASA Technical Reports Server (NTRS)

    Pellicori, S. F.; Russell, E. E.; Watts, L. A.

    1973-01-01

    The imaging photopolarimeter aboard the Pioneer 10 spacecraft en route to the vicinity of Jupiter is described. This instrument is capable of moderate resolution spin-scan imaging and high precision polarimetric and photometric mapping of Jupiter in red and blue light. The field of view can be selectively changed from 0.50 mrad square to 40 mrad square to accommodate resolution and radiance combinations ranging from the zodiacal background to that of Jupiter. Optical materials were chosen to survive, with minimum degradation, the rigors of a nearly 2-year journey to Jupiter including transit through the Jovian trapped radiation belts. The optics are described in detail, and the operational system is outlined. The procedures for preflight and in-flight calibration are described, and some performance characteristics and preliminary flight results are presented.

  10. Pioneer imaging photopolarimeter optical system.

    PubMed

    Pellicori, S F; Russell, E E; Watts, L A

    1973-06-01

    The imaging photopolarimeter aboard the Pioneer 10 spacecraft en route to the vicinity of Jupiter is described. This instrument is capable of moderate resolution spin-scan imaging and high precision polarimetric and photometric mapping of Jupiter in red and blue light. The field of view can be selectively changed from 0.50 mrad square to 40 mrad square to accommodate resolution and radiance combinations ranging from the zodiacal background to that of Jupiter. The dynamic range (radiance) of the instrument is greater than 10(8). Optical materials were chosen to survive, with minimum degradation, the rigors of a nearly 2-year journey to Jupiter including transit through the Jovian trapped radiation belts. The optics are described in detail, and the operational system is outlined. The procedures for preflight and in-flight calibration are described, and some performance characteristics and preliminary flight results are presented. PMID:20125507

  11. Contrast enhancement in microscopy of human thyroid tumors by means of acousto-optic adaptive spatial filtering

    NASA Astrophysics Data System (ADS)

    Yushkov, Konstantin B.; Molchanov, Vladimir Y.; Belousov, Pavel V.; Abrosimov, Aleksander Y.

    2016-01-01

    We report a method for edge enhancement in the images of transparent samples using analog image processing in coherent light. The experimental technique is based on adaptive spatial filtering with an acousto-optic tunable filter in a telecentric optical system. We demonstrate processing of microscopic images of unstained and stained histological sections of human thyroid tumor with improved contrast.

  12. Fluorescence imaging spectrometer optical design

    NASA Astrophysics Data System (ADS)

    Taiti, A.; Coppo, P.; Battistelli, E.

    2015-09-01

    The optical design of the FLuORescence Imaging Spectrometer (FLORIS) studied for the Fluorescence Explorer (FLEX) mission is discussed. FLEX is a candidate for the ESA's 8th Earth Explorer opportunity mission. FLORIS is a pushbroom hyperspectral imager foreseen to be embarked on board of a medium size satellite, flying in tandem with Sentinel-3 in a Sun synchronous orbit at a height of about 815 km. FLORIS will observe the vegetation fluorescence and reflectance within a spectral range between 500 and 780 nm. Multi-frames acquisitions on matrix detectors during the satellite movement will allow the production of 2D Earth scene images in two different spectral channels, called HR and LR with spectral resolution of 0.3 and 2 nm respectively. A common fore optics is foreseen to enhance by design the spatial co-registration between the two spectral channels, which have the same ground spatial sampling (300 m) and swath (150 km). An overlapped spectral range between the two channels is also introduced to simplify the spectral coregistration. A compact opto-mechanical solution with all spherical and plane optical elements is proposed, and the most significant design rationales are described. The instrument optical architecture foresees a dual Babinet scrambler, a dioptric telescope and two grating spectrometers (HR and LR), each consisting of a modified Offner configuration. The developed design is robust, stable vs temperature, easy to align, showing very high optical quality along the whole field of view. The system gives also excellent correction for transverse chromatic aberration and distortions (keystone and smile).

  13. Durham adaptive optics real-time controller.

    PubMed

    Basden, Alastair; Geng, Deli; Myers, Richard; Younger, Eddy

    2010-11-10

    The Durham adaptive optics (AO) real-time controller was initially a proof of concept design for a generic AO control system. It has since been developed into a modern and powerful central-processing-unit-based real-time control system, capable of using hardware acceleration (including field programmable gate arrays and graphical processing units), based primarily around commercial off-the-shelf hardware. It is powerful enough to be used as the real-time controller for all currently planned 8 m class telescope AO systems. Here we give details of this controller and the concepts behind it, and report on performance, including latency and jitter, which is less than 10 μs for small AO systems.

  14. Wavefront Control for Extreme Adaptive Optics

    SciTech Connect

    Poyneer, L A

    2003-07-16

    Current plans for Extreme Adaptive Optics systems place challenging requirements on wave-front control. This paper focuses on control system dynamics, wave-front sensing and wave-front correction device characteristics. It may be necessary to run an ExAO system after a slower, low-order AO system. Running two independent systems can result in very good temporal performance, provided specific design constraints are followed. The spatially-filtered wave-front sensor, which prevents aliasing and improves PSF sensitivity, is summarized. Different models of continuous and segmented deformable mirrors are studied. In a noise-free case, a piston-tip-tilt segmented MEMS device can achieve nearly equivalent performance to a continuous-sheet DM in compensating for a static phase aberration with use of spatial filtering.

  15. Adaptive compressed sensing for spectral-domain optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Wang, Yi; Chen, Xiaodong; Wang, Ting; Li, Hongxiao; Yu, Daoyin

    2014-03-01

    Spectral-domain optical coherence tomography (SD-OCT) is a non-contact and non-invasive method for measuring the change of biological tissues caused by pathological changes of body. CCD with huge number of pixels is usually used in SD-OCT to increase the detecting depth, thus enhancing the hardness of data transmission and storage. The usage of compressed sensing (CS) in SD-OCT is able to reduce the trouble of large data transfer and storage, thus eliminating the complexity of processing system. The traditional CS uses the same sampling model for SD-OCT images of different tissue, leading to reconstruction images with different quality. We proposed a CS with adaptive sampling model. The new model is based on uniform sampling model, and the interference spectral of SD-OCT is considered to adjust the local sampling ratio. Compared with traditional CS, adaptive CS can modify the sampling model for images of different tissue according to different interference spectral, getting reconstruction images with high quality without changing sampling model.

  16. Design and progress toward a multi-conjugate adaptive optics system for distributed aberration correction

    SciTech Connect

    Baker, K; Olivier, S; Tucker, J; Silva, D; Gavel, D; Lim, R; Gratrix, E

    2004-08-17

    This article investigates the use of a multi-conjugate adaptive optics system to improve the field-of-view for the system. The emphasis of this research is to develop techniques to improve the performance of optical systems with applications to horizontal imaging. The design and wave optics simulations of the proposed system are given. Preliminary results from the multi-conjugate adaptive optics system are also presented. The experimental system utilizes a liquid-crystal spatial light modulator and an interferometric wave-front sensor for correction and sensing of the phase aberrations, respectively.

  17. Adaptive optics without altering visual perception.

    PubMed

    Koenig, D E; Hart, N W; Hofer, H J

    2014-04-01

    Adaptive optics combined with visual psychophysics creates the potential to study the relationship between visual function and the retina at the cellular scale. This potential is hampered, however, by visual interference from the wavefront-sensing beacon used during correction. For example, we have previously shown that even a dim, visible beacon can alter stimulus perception (Hofer et al., 2012). Here we describe a simple strategy employing a longer wavelength (980nm) beacon that, in conjunction with appropriate restriction on timing and placement, allowed us to perform psychophysics when dark adapted without altering visual perception. The method was verified by comparing detection and color appearance of foveally presented small spot stimuli with and without the wavefront beacon present in 5 subjects. As an important caution, we found that significant perceptual interference can occur even with a subliminal beacon when additional measures are not taken to limit exposure. Consequently, the lack of perceptual interference should be verified for a given system, and not assumed based on invisibility of the beacon.

  18. Adaptive Detector Arrays for Optical Communications Receivers

    NASA Technical Reports Server (NTRS)

    Vilnrotter, V.; Srinivasan, M.

    2000-01-01

    The structure of an optimal adaptive array receiver for ground-based optical communications is described and its performance investigated. Kolmogorov phase screen simulations are used to model the sample functions of the focal-plane signal distribution due to turbulence and to generate realistic spatial distributions of the received optical field. This novel array detector concept reduces interference from background radiation by effectively assigning higher confidence levels at each instant of time to those detector elements that contain significant signal energy and suppressing those that do not. A simpler suboptimum structure that replaces the continuous weighting function of the optimal receiver by a hard decision on the selection of the signal detector elements also is described and evaluated. Approximations and bounds to the error probability are derived and compared with the exact calculations and receiver simulation results. It is shown that, for photon-counting receivers observing Poisson-distributed signals, performance improvements of approximately 5 dB can be obtained over conventional single-detector photon-counting receivers, when operating in high background environments.

  19. Extreme Adaptive Optics Testbed: Results and Future Work

    SciTech Connect

    Evans, J W; Sommargren, G; Poyneer, L; Macintosh, B; Severson, S; Dillon, D; Sheinis, A; Palmer, D; Kasdin, J; Olivier, S

    2004-07-15

    'Extreme' adaptive optics systems are optimized for ultra-high-contrast applications, such as ground-based extrasolar planet detection. The Extreme Adaptive Optics Testbed at UC Santa Cruz is being used to investigate and develop technologies for high-contrast imaging, especially wavefront control. A simple optical design allows us to minimize wavefront error and maximize the experimentally achievable contrast before progressing to a more complex set-up. A phase shifting diffraction interferometer is used to measure wavefront errors with sub-nm precision and accuracy. We have demonstrated RMS wavefront errors of <1.3 nm and a contrast of >10{sup -7} over a substantial region using a shaped pupil. Current work includes the installation and characterization of a 1024-actuator Micro-Electro-Mechanical- Systems (MEMS) deformable mirror, manufactured by Boston Micro-Machines, which will be used for wavefront control. In our initial experiments we can flatten the deformable mirror to 1.8-nm RMS wavefront error within a control radius of 5-13 cycles per aperture. Ultimately this testbed will be used to test all aspects of the system architecture for an extrasolar planet-finding AO system.

  20. Block adaptive rate controlled image data compression

    NASA Technical Reports Server (NTRS)

    Rice, R. F.; Hilbert, E.; Lee, J.-J.; Schlutsmeyer, A.

    1979-01-01

    A block adaptive rate controlled (BARC) image data compression algorithm is described. It is noted that in the algorithm's principal rate controlled mode, image lines can be coded at selected rates by combining practical universal noiseless coding techniques with block adaptive adjustments in linear quantization. Compression of any source data at chosen rates of 3.0 bits/sample and above can be expected to yield visual image quality with imperceptible degradation. Exact reconstruction will be obtained if the one-dimensional difference entropy is below the selected compression rate. It is noted that the compressor can also be operated as a floating rate noiseless coder by simply not altering the input data quantization. Here, the universal noiseless coder ensures that the code rate is always close to the entropy. Application of BARC image data compression to the Galileo orbiter mission of Jupiter is considered.

  1. Measurements of contrast sensitivity by an adaptive optics visual simulator

    NASA Astrophysics Data System (ADS)

    Yamaguchi, Tatsuo; Ucikawa, Keiji

    2015-08-01

    We developed an adaptive optics visual simulator (AOVS) to study the relationship between the contrast sensitivity and higher-order wavefront aberrations of human eyes. A desired synthetic aberration was virtually generated on a subject eye by the AOVS, and red laser light was used to measure the aberrations. The contrast sensitivity was measured in a psychophysical experiment using visual stimulus patterns provided by a large-contrast-range imaging system, which included two liquid crystal displays illuminated by red light emitting diodes from the backside. The diameter of the pupil was set to 4 mm by an artificial aperture, and the retinal illuminance of the stimulus image was controlled to 10 Td. Experiments conducted with four normal subjects revealed that their contrast sensitivity to a high-spatial-frequency vertical sinusoidal grating pattern was lower in the presence of a horizontal coma aberration than in the presence of a vertical coma or no aberrations ( p < 0.02, Nagai method).

  2. Adaptation and visual search in mammographic images.

    PubMed

    Kompaniez-Dunigan, Elysse; Abbey, Craig K; Boone, John M; Webster, Michael A

    2015-05-01

    Radiologists face the visually challenging task of detecting suspicious features within the complex and noisy backgrounds characteristic of medical images. We used a search task to examine whether the salience of target features in x-ray mammograms could be enhanced by prior adaptation to the spatial structure of the images. The observers were not radiologists, and thus had no diagnostic training with the images. The stimuli were randomly selected sections from normal mammograms previously classified with BIRADS Density scores of "fatty" versus "dense," corresponding to differences in the relative quantities of fat versus fibroglandular tissue. These categories reflect conspicuous differences in visual texture, with dense tissue being more likely to obscure lesion detection. The targets were simulated masses corresponding to bright Gaussian spots, superimposed by adding the luminance to the background. A single target was randomly added to each image, with contrast varied over five levels so that they varied from difficult to easy to detect. Reaction times were measured for detecting the target location, before or after adapting to a gray field or to random sequences of a different set of dense or fatty images. Observers were faster at detecting the targets in either dense or fatty images after adapting to the specific background type (dense or fatty) that they were searching within. Thus, the adaptation led to a facilitation of search performance that was selective for the background texture. Our results are consistent with the hypothesis that adaptation allows observers to more effectively suppress the specific structure of the background, thereby heightening visual salience and search efficiency.

  3. UWGSP7: a real-time optical imaging workstation

    NASA Astrophysics Data System (ADS)

    Bush, John E.; Kim, Yongmin; Pennington, Stan D.; Alleman, Andrew P.

    1995-04-01

    With the development of UWGSP7, the University of Washington Image Computing Systems Laboratory has a real-time workstation for continuous-wave (cw) optical reflectance imaging. Recent discoveries in optical science and imaging research have suggested potential practical use of the technology as a medical imaging modality and identified the need for a machine to support these applications in real time. The UWGSP7 system was developed to provide researchers with a high-performance, versatile tool for use in optical imaging experiments with the eventual goal of bringing the technology into clinical use. One of several major applications of cw optical reflectance imaging is tumor imaging which uses a light-absorbing dye that preferentially sequesters in tumor tissue. This property could be used to locate tumors and to identify tumor margins intraoperatively. Cw optical reflectance imaging consists of illumination of a target with a band-limited light source and monitoring the light transmitted by or reflected from the target. While continuously illuminating the target, a control image is acquired and stored. A dye is injected into a subject and a sequence of data images are acquired and processed. The data images are aligned with the control image and then subtracted to obtain a signal representing the change in optical reflectance over time. This signal can be enhanced by digital image processing and displayed in pseudo-color. This type of emerging imaging technique requires a computer system that is versatile and adaptable. The UWGSP7 utilizes a VESA local bus PC as a host computer running the Windows NT operating system and includes ICSL developed add-on boards for image acquisition and processing. The image acquisition board is used to digitize and format the analog signal from the input device into digital frames and to the average frames into images. To accommodate different input devices, the camera interface circuitry is designed in a small mezzanine board

  4. Analysis of optical wavefront reconstruction and deconvolution in adaptive optics

    NASA Astrophysics Data System (ADS)

    Luke, David Russell

    2001-11-01

    It was in the spirit of ``reuniting divergent trends by clarifying the common features and interconnections of many distinct and diverse scientific facts'' that Courant and Hilbert published their book Methods of Mathematical Physics [43]. This thesis is written in the same spirit and with the same goal. We focus our attention on the problem of wavefront reconstruction and deconvolution in adaptive optics. This is an ill-posed, non-linear inverse problem that touches on the theory of harmonic analysis, variational analysis, signal processing, nonconvex optimization, regularization, statistics and probability. Numerical solutions rely on spectral and operator-splitting methods as well as limited memory and multi-resolution techniques. We introduce novel methods for wavefront reconstruction and compare our results against common techniques. Previous work on this problem is reviewed and unified in a non-parametric, analytic framework.

  5. Liquid-crystal prisms for tip-tilt adaptive optics.

    PubMed

    Love, G D; Major, J V; Purvis, A

    1994-08-01

    Results from an electrically addressed liquid-crystal cell producing continuous phase profiles are presented. The adaptive deflection of a beam of light for use in a tip-tilt adaptive optics system is demonstrated. We compare the optical performance of liquid-crystal prisms with experimental data on atmospheric seeing at the William Herschel Telescope.

  6. Liquid-crystal prisms for tip-tilt adaptive optics.

    PubMed

    Love, G D; Major, J V; Purvis, A

    1994-08-01

    Results from an electrically addressed liquid-crystal cell producing continuous phase profiles are presented. The adaptive deflection of a beam of light for use in a tip-tilt adaptive optics system is demonstrated. We compare the optical performance of liquid-crystal prisms with experimental data on atmospheric seeing at the William Herschel Telescope. PMID:19844566

  7. Compact MEMS-based Adaptive Optics Optical Coherence Tomography for Clinical Use

    SciTech Connect

    Chen, D; Olivier, S; Jones, S; Zawadzki, R; Evans, J; Choi, S; Werner, J

    2008-02-04

    We describe a compact MEMS-based adaptive optics (AO) optical coherence tomography system with improved AO performance and ease of clinical use. A typical AO system consists of a Shack-Hartmann wavefront sensor and a deformable mirror that measures and corrects the ocular and system aberrations. Because of the limitation on the current deformable mirror technologies, the amount of real-time ocular-aberration compensation is restricted and small in the previous AO-OCT instruments. In this instrument, we proposed to add an optical apparatus to correct the spectacle aberrations of the patients such as myopia, hyperopia and astigmatism. This eliminated the tedious process of the trial lenses in clinical imaging. Different amount of spectacle aberration compensation was achieved by motorized stages and automated with the AO computer for ease of clinical use. In addition, the compact AO-OCT was optimized to have minimum system aberrations to reduce AO registration errors and improve AO performance.

  8. Compact MEMS-based adaptive optics: optical coherence tomography for clinical use

    NASA Astrophysics Data System (ADS)

    Chen, Diana C.; Olivier, Scot S.; Jones, Steven M.; Zawadzki, Robert J.; Evans, Julia W.; Choi, Stacey S.; Werner, John S.

    2008-02-01

    We describe a compact MEMS-based adaptive optics (AO) optical coherence tomography (OCT) system with improved AO performance and ease of clinical use. A typical AO system consists of a Shack-Hartmann wavefront sensor and a deformable mirror that measures and corrects the ocular and system aberrations. Because of limitations on current deformable mirror technologies, the amount of real-time ocular-aberration compensation is restricted and small in previous AO-OCT instruments. In this instrument, we incorporate an optical apparatus to correct the spectacle aberrations of the patients such as myopia, hyperopia and astigmatism. This eliminates the tedious process of using trial lenses in clinical imaging. Different amount of spectacle aberration compensation was achieved by motorized stages and automated with the AO computer for ease of clinical use. In addition, the compact AO-OCT was optimized to have minimum system aberrations to reduce AO registration errors and improve AO performance.

  9. CRAO: a compact and refractive adaptive-optics

    NASA Astrophysics Data System (ADS)

    Fujishiro, Naofumi; Kitao, Eiji; Shimizu, Tomo; Matsui, Takuya; Ikeda, Yuji; Kawakita, Hideyo; Oya, Shin

    2014-08-01

    CRAO is a demonstrator of a compact and low-cost adaptive-optics (AO) with a double-pass lens configuration. Owing to its compact optical layout compared to conventional reflective AOs, the instrument size can be reduced to only 0.03 square meters. We plan to apply this miniaturization technique into future AOs on a variety of telescopes ranging from 1m- to 30m-class. CRAO is installed at a Nasmyth focus of the 1.3m Araki telescope at Koyama Astronomical Observatory in Kyoto Sangyo University. CRAO adopts a closed-loop single-conjugate system with wavelength coverage of 400 - 700 nm and the field of view of 30 arcsec. For low cost, we also employ commercial products on its wavefront sensor (WFS), deformable mirror (DM), and tip-tilt (TT) stage. CRAO is designed to improve the atmospheric seeing from 2.5 to 0.6arcsec under a typical condition at Koyama Astronomical Observatory with 12x12 subapertures in the WFS, 48 electrodes in the membrane DM and the control bandwidth of 200Hz. In order to examine key issues inherent in refractive optical system such as chromatic aberration, temperature aberration and ghost images, room and on-sky experiments are currently underway. CRAO has seen first light in May 2014, and we have confirmed that effects of chromatic aberration and ghost images induced by its refractive optics are negligible for at least TT correction. In this paper, we present experimental results as well as the design of optics, opto-mechanics and control system.

  10. Optimized micromirror arrays for adaptive optics

    NASA Astrophysics Data System (ADS)

    Michalicek, M. Adrian; Comtois, John H.; Hetherington, Dale L.

    1999-01-01

    This paper describes the design, layout, fabrication, and surface characterization of highly optimized surface micromachined micromirror devices. Design considerations and fabrication capabilities are presented. These devices are fabricated in the state-of-the-art, four-level, planarized, ultra-low-stress polysilicon process available at Sandia National Laboratories known as the Sandia Ultra-planar Multi-level MEMS Technology (SUMMiT). This enabling process permits the development of micromirror devices with near-ideal characteristics that have previously been unrealizable in standard three-layer polysilicon processes. The reduced 1 μm minimum feature sizes and 0.1 μm mask resolution make it possible to produce dense wiring patterns and irregularly shaped flexures. Likewise, mirror surfaces can be uniquely distributed and segmented in advanced patterns and often irregular shapes in order to minimize wavefront error across the pupil. The ultra-low-stress polysilicon and planarized upper layer allow designers to make larger and more complex micromirrors of varying shape and surface area within an array while maintaining uniform performance of optical surfaces. Powerful layout functions of the AutoCAD editor simplify the design of advanced micromirror arrays and make it possible to optimize devices according to the capabilities of the fabrication process. Micromirrors fabricated in this process have demonstrated a surface variance across the array from only 2-3 nm to a worst case of roughly 25 nm while boasting active surface areas of 98% or better. Combining the process planarization with a ``planarized-by-design'' approach will produce micromirror array surfaces that are limited in flatness only by the surface deposition roughness of the structural material. Ultimately, the combination of advanced process and layout capabilities have permitted the fabrication of highly optimized micromirror arrays for adaptive optics.

  11. Wave-Optics Analysis of Pupil Imaging

    NASA Technical Reports Server (NTRS)

    Dean, Bruce H.; Bos, Brent J.

    2006-01-01

    Pupil imaging performance is analyzed from the perspective of physical optics. A multi-plane diffraction model is constructed by propagating the scalar electromagnetic field, surface by surface, along the optical path comprising the pupil imaging optical system. Modeling results are compared with pupil images collected in the laboratory. The experimental setup, although generic for pupil imaging systems in general, has application to the James Webb Space Telescope (JWST) optical system characterization where the pupil images are used as a constraint to the wavefront sensing and control process. Practical design considerations follow from the diffraction modeling which are discussed in the context of the JWST Observatory.

  12. Adaptive Optics Facility Status Report: When First Light Is Produced Rather Than Captured

    NASA Astrophysics Data System (ADS)

    Arsenault, R.; Madec, P.-Y.; Vernet, E.; Hackenberg, W.; Bonaccini Calia, D.; La Penna, P.; Paufique, J.; Kuntschner, H.; Pirard, J.-F.; Sarazin, M.; Haguenauer, P.; Hubin, N.; Vera, I.

    2016-06-01

    First light for the 4 Laser Guide Star Facility (4LGSF) took place in Paranal on 26 April 2016 with four laser units in operation for the first time. A combined test with the first laser guide star unit and the Ground Layer Adaptive optics Assisted by Lasers (GRAAL) instrument in October 2015 demonstrated the whole acquisition sequence of the Adaptive Optics Facility (AOF). Many tools that will support the operation of the AOF for science observations have meanwhile been implemented. GALACSI was granted Provisional Acceptance in Europe in April 2016, completing the system tests and qualification in Garching of the adaptive optics modules GRAAL and GALACSI (Ground Atmospheric Layer Adaptive Optics for Spectroscopic Imaging), their real-time computers and the deformable secondary mirror (DSM). Results of tests both in the laboratory and on sky are presented. The installation of the DSM and GALACSI will be completed by early 2017, to be followed by commissioning of all AOF systems.

  13. AVES: an adaptive optics visual echelle spectrograph for the VLT

    NASA Astrophysics Data System (ADS)

    Pasquini, Luca; Delabre, Bernard; Avila, Gerardo; Bonaccini, Domenico

    1998-07-01

    We present the preliminary study of a low cost, high performance spectrograph for the VLT, for observations in the V, R and I bands. This spectrograph is meant for intermediate (R equals 16,000) resolution spectroscopy of faint (sky and/or detector limited) sources, with particular emphasis on the study of solar-type (F-G) stars belonging to the nearest galaxies and to distant (or highly reddened) galactic clusters. The spectrograph is designed to use the adaptive optics (AO) systems at the VLT Telescope. Even if these AO systems will not provide diffraction limited images in the V, R and I bands, the photon concentration will still be above approximately 60% of the flux in an 0.3 arcsecond aperture for typical Paranal conditions. This makes the construction of a compact, cheap and efficient echelle spectrograph possible. AVES will outperform comparable non adaptive optic instruments by more than one magnitude for sky- and/or detector-limited observations, and it will be very suitable for observations in crowded fields.

  14. Non-common path aberration correction in an adaptive optics scanning ophthalmoscope.

    PubMed

    Sulai, Yusufu N; Dubra, Alfredo

    2014-09-01

    The correction of non-common path aberrations (NCPAs) between the imaging and wavefront sensing channel in a confocal scanning adaptive optics ophthalmoscope is demonstrated. NCPA correction is achieved by maximizing an image sharpness metric while the confocal detection aperture is temporarily removed, effectively minimizing the monochromatic aberrations in the illumination path of the imaging channel. Comparison of NCPA estimated using zonal and modal orthogonal wavefront corrector bases provided wavefronts that differ by ~λ/20 in root-mean-squared (~λ/30 standard deviation). Sequential insertion of a cylindrical lens in the illumination and light collection paths of the imaging channel was used to compare image resolution after changing the wavefront correction to maximize image sharpness and intensity metrics. Finally, the NCPA correction was incorporated into the closed-loop adaptive optics control by biasing the wavefront sensor signals without reducing its bandwidth.

  15. Non-common path aberration correction in an adaptive optics scanning ophthalmoscope

    PubMed Central

    Sulai, Yusufu N.; Dubra, Alfredo

    2014-01-01

    The correction of non-common path aberrations (NCPAs) between the imaging and wavefront sensing channel in a confocal scanning adaptive optics ophthalmoscope is demonstrated. NCPA correction is achieved by maximizing an image sharpness metric while the confocal detection aperture is temporarily removed, effectively minimizing the monochromatic aberrations in the illumination path of the imaging channel. Comparison of NCPA estimated using zonal and modal orthogonal wavefront corrector bases provided wavefronts that differ by ~λ/20 in root-mean-squared (~λ/30 standard deviation). Sequential insertion of a cylindrical lens in the illumination and light collection paths of the imaging channel was used to compare image resolution after changing the wavefront correction to maximize image sharpness and intensity metrics. Finally, the NCPA correction was incorporated into the closed-loop adaptive optics control by biasing the wavefront sensor signals without reducing its bandwidth. PMID:25401020

  16. An Adaptive Optics Survey for Companions to Stars with Extra-Solar Planets

    SciTech Connect

    Lloyd, J.P.; Liu, M.C.; Graham, J.R.; Enoch, M.; Kalas, P.; Marcy, G.W.; Fischer, D.; Patience, J.; Macintosh, B.; Gavel, D.T.; Olivier, S.S.; Max, C.E.; White, R.; Ghez, A.M.; McLean, I.S.

    2000-11-27

    We have undertaken an adaptive optics imaging survey of extrasolar planetary systems and stars showing interesting radial velocity trends from high precision radial velocity searches. Adaptive Optics increases the resolution and dynamic range of an image, substantially improving the detectability of faint close companions. This survey is sensitive to objects less luminous than the bottom of the main sequence at separations as close as 1 inch. We have detected stellar companions to the planet bearing stars HD 114762 and Tau Boo. We have also detected a companion to the non-planet bearing star 16 Cyg A.

  17. Adaptive Optics Survey for Companions to stars with Extra-Solar Planets

    SciTech Connect

    Lloyd, J P; Liu, M C; Graham, J R; Enoch, M; Kalas, P; Marcy, G W; Fischer, D; Patience, J; Macintosh, B; Gavel, D T; Olivier, S S; Max, C E; White, R; Ghez, A M; McLean, I S

    2000-11-27

    We have undertaken an adaptive optics imaging survey of extrasolar planetary systems and stars showing interesting radial velocity trends from high precision radial velocity searches. Adaptive Optics increases the resolution and dynamic range of an image, substantially improving the detectability of faint close companions. This survey is sensitive to objects less luminous than the bottom of the main sequence at separations as close as 1 inch. We have detected stellar companions to the planet bearing stars HD 114762 and Tau Boo. We have also detected a companion to the non-planet bearing star 16 Cyg A.

  18. Performance evaluation of a sensorless adaptive optics multiphoton microscope.

    PubMed

    Skorsetz, Martin; Artal, Pablo; Bueno, Juan M

    2016-03-01

    A wavefront sensorless adaptive optics technique was combined with a custom-made multiphoton microscope to correct for specimen-induced aberrations. A liquid-crystal-on-silicon (LCoS) modulator was used to systematically generate Zernike modes during image recording. The performance of the instrument was evaluated in samples providing different nonlinear signals and the benefit of correcting higher order aberrations was always noticeable (in both contrast and resolution). The optimum aberration pattern was stable in time for the samples here involved. For a particular depth location within the sample, the wavefront to be precompensated was independent on the size of the imaged area (up to ∼ 360 × 360 μm(2)). The mode combination optimizing the recorded image depended on the Zernike correction control sequence; however, the final images hardly differed. At deeper locations, a noticeable dominance of spherical aberration was found. The influence of other aberration terms was also compared to the effect of the spherical aberration. PMID:26469361

  19. Identification of nonlinear optical systems using adaptive kernel methods

    NASA Astrophysics Data System (ADS)

    Wang, Xiaodong; Zhang, Changjiang; Zhang, Haoran; Feng, Genliang; Xu, Xiuling

    2005-12-01

    An identification approach of nonlinear optical dynamic systems, based on adaptive kernel methods which are modified version of least squares support vector machine (LS-SVM), is presented in order to obtain the reference dynamic model for solving real time applications such as adaptive signal processing of the optical systems. The feasibility of this approach is demonstrated with the computer simulation through identifying a Bragg acoustic-optical bistable system. Unlike artificial neural networks, the adaptive kernel methods possess prominent advantages: over fitting is unlikely to occur by employing structural risk minimization criterion, the global optimal solution can be uniquely obtained owing to that its training is performed through the solution of a set of linear equations. Also, the adaptive kernel methods are still effective for the nonlinear optical systems with a variation of the system parameter. This method is robust with respect to noise, and it constitutes another powerful tool for the identification of nonlinear optical systems.

  20. Probing other solar systems with current and future adaptive optics

    SciTech Connect

    Macintosh, B; Marois, C; Phillion, D; Poyneer, L; Graham, J; Zuckerman, B; Gavel, D; Veran, J; Wilhelmsen-Evans, J; Mellis, C

    2008-09-08

    Over the past decade, the study of extrasolar planets through indirect techniques--primarily Doppler measurements--has revolutionized our understanding of other solar systems. The next major step in this field will be the direct detection and characterization, via imaging and spectroscopy, of the planets themselves. To achieve this, we must separate the light from the faint planet from the extensive glare of its parent star. We pursued this goal using the current generation of adaptive optics (AO) systems on large ground-based telescopes, using infrared imaging to search for the thermal emission from young planets and developing image processing techniques to distinguish planets from telescope-induced artifacts. Our new Angular Differential Imaging (ADI) technique, which uses the sidereal rotation of the Earth and telescope, is now standard for ground-based high-contrast imaging. Although no young planets were found in our surveys, we placed the strongest limits yet on giant planets in wide orbits (>30 AU) around young stars and characterized planetary companion candidates. The imaging of planetary companions on solar-system-like scales (5-30 AU) will require a new generation of advanced AO systems that are an order of magnitude more powerful than the LLNL-built Keck AO system. We worked to develop and test the key technologies needed for these systems, including a spatially-filtered wavefront sensor, efficient and accurate wavefront reconstruction algorithms, and precision AO wavefront control at the sub-nm level. LLNL has now been selected by the Gemini Observatory to lead the construction of the Gemini Planet Imager, a $24M instrument that will be the most advanced AO system in the world.

  1. The 2004 Opposition of Ceres Observed with Adaptive Optics on the VLT

    NASA Technical Reports Server (NTRS)

    Erard, S.; Frorni, O.; Ollivier, M.; Dotto, E.; Roush, T.; Poulet, F.; Mueller, T.

    2005-01-01

    The close opposition of Ceres in January 2004 has been observed with the NACO adaptive optics system on the VLT. Both imaging and spectroscopy were performed in the 1.1-4.1 m range. Extensive longitudinal coverage was acquired during a three days run, with spatial resolution up to 50 km in imaging mode. The scientific objectives are 1) to provide the first IR map of Ceres; 2) to map possible compositional variations at the surface. Only imaging results are presented here.

  2. Reflective optical imaging system with balanced distortion

    DOEpatents

    Chapman, Henry N.; Hudyma, Russell M.; Shafer, David R.; Sweeney, Donald W.

    1999-01-01

    An optical system compatible with short wavelength (extreme ultraviolet) An optical system compatible with short wavelength (extreme ultraviolet) radiation comprising four reflective elements for projecting a mask image onto a substrate. The four optical elements comprise, in order from object to image, convex, concave, convex and concave mirrors. The optical system is particularly suited for step and scan lithography methods. The invention enables the use of larger slit dimensions associated with ring field scanning optics, improves wafer throughput and allows higher semiconductor device density. The inventive optical system is characterized by reduced dynamic distortion because the static distortion is balanced across the slit width.

  3. Adaptive optics ophthalmologic systems using dual deformable mirrors

    SciTech Connect

    Jones, S; Olivier, S; Chen, D; Sadda, S; Joeres, S; Zawadzki, R; Werner, J S; Miller, D

    2007-02-01

    Adaptive Optics (AO) have been increasingly combined with a variety of ophthalmic instruments over the last decade to provide cellular-level, in-vivo images of the eye. The use of MEMS deformable mirrors in these instruments has recently been demonstrated to reduce system size and cost while improving performance. However, currently available MEMS mirrors lack the required range of motion for correcting large ocular aberrations, such as defocus and astigmatism. In order to address this problem, we have developed an AO system architecture that uses two deformable mirrors, in a woofer/tweeter arrangement, with a bimorph mirror as the woofer and a MEMS mirror as the tweeter. This setup provides several advantages, including extended aberration correction range, due to the large stroke of the bimorph mirror, high order aberration correction using the MEMS mirror, and additionally, the ability to ''focus'' through the retina. This AO system architecture is currently being used in four instruments, including an Optical Coherence Tomography (OCT) system and a retinal flood-illuminated imaging system at the UC Davis Medical Center, a Scanning Laser Ophthalmoscope (SLO) at the Doheny Eye Institute, and an OCT system at Indiana University. The design, operation and evaluation of this type of AO system architecture will be presented.

  4. Modelling global multi-conjugated adaptive optics

    NASA Astrophysics Data System (ADS)

    Viotto, Valentina; Ragazzoni, Roberto; Magrin, Demetrio; Bergomi, Maria; Dima, Marco; Farinato, Jacopo; Marafatto, Luca; Greggio, Davide

    2014-08-01

    The recently proposed concept of Global MCAO (GMCAO) aims to look for Natural Guide Stars in a very wide technical Field of View (FoV), to increase the overall sky coverage, and deals with the consequent depth of focus reduction introducing numerically a quite-high number of Virtual Deformable Mirrors (VDMs), which are then the starting point for an optimization of the real DMs shapes for the correction of the -smaller- scientific FoV. To translate the GMCAO concept into a real system, a number of parameters requires to be analyzed and optimized, like the number of references and VDMs to be used, the technical FoV size, the spatial samplings, the sensing wavelength. These and some other major choices, like the open loop WFSs concept and design, will then drive the requirements and the performance of the system (e.g. limiting magnitude, linear response, and sensitivity). This paper collects some major results of the on-going study on the feasibility of an Adaptive Optics system for the E-ELT, based on GMCAO, with a particular emphasis on the sky coverage issue. Besides the sensitivity analysis of the optimization of the already mentioned parameters, such a topic involves the implementation of an IDL code simulation tool to estimate the system performance in terms of Strehl Ratio in a 2×2 arcmin FoV, when a variable number of NGSs and VDMs are used. Different technical FoV diameters for the references selection and various constellations can be also compared. This study could be the starting point for a dedicated laboratory testing and, in the future, an on-sky experiment at an 8m telescope with a "scaled down" demonstrator.

  5. Reduction of laser spot elongation in adaptive optics.

    PubMed

    Ribak, Erez N; Ragazzoni, Roberto

    2004-06-15

    Adaptive optics systems measure the wave front to be corrected by use of a reference source, a star, or a laser beacon. Such laser guide stars are a few kilometers long, and when observed near the edges of large telescopes they appear elongated. This limits their utility significantly. However, with more sophisticated launch optics their shape and length can be controlled. We propose to string around the rim of a telescope a number of small telescopes that will add laser beams in the scattering medium to create a compact spot. The method could also be adapted for ocular adaptive optics.

  6. Kalman filtering to suppress spurious signals in Adaptive Optics control

    SciTech Connect

    Poyneer, L; Veran, J P

    2010-03-29

    In many scenarios, an Adaptive Optics (AO) control system operates in the presence of temporally non-white noise. We use a Kalman filter with a state space formulation that allows suppression of this colored noise, hence improving residual error over the case where the noise is assumed to be white. We demonstrate the effectiveness of this new filter in the case of the estimated Gemini Planet Imager tip-tilt environment, where there are both common-path and non-common path vibrations. We discuss how this same framework can also be used to suppress spatial aliasing during predictive wavefront control assuming frozen flow in a low-order AO system without a spatially filtered wavefront sensor, and present experimental measurements from Altair that clearly reveal these aliased components.

  7. Advances in optical imaging for pharmacological studies

    PubMed Central

    Arranz, Alicia; Ripoll, Jorge

    2015-01-01

    Imaging approaches are an essential tool for following up over time representative parameters of in vivo models, providing useful information in pharmacological studies. Main advantages of optical imaging approaches compared to other imaging methods are their safety, straight-forward use and cost-effectiveness. A main drawback, however, is having to deal with the presence of high scattering and high absorption in living tissues. Depending on how these issues are addressed, three different modalities can be differentiated: planar imaging (including fluorescence and bioluminescence in vivo imaging), optical tomography, and optoacoustic approaches. In this review we describe the latest advances in optical in vivo imaging with pharmacological applications, with special focus on the development of new optical imaging probes in order to overcome the strong absorption introduced by different tissue components, especially hemoglobin, and the development of multimodal imaging systems in order to overcome the resolution limitations imposed by scattering. PMID:26441646

  8. Self-adaptive iris image acquisition system

    NASA Astrophysics Data System (ADS)

    Dong, Wenbo; Sun, Zhenan; Tan, Tieniu; Qiu, Xianchao

    2008-03-01

    Iris image acquisition is the fundamental step of the iris recognition, but capturing high-resolution iris images in real-time is very difficult. The most common systems have small capture volume and demand users to fully cooperate with machines, which has become the bottleneck of iris recognition's application. In this paper, we aim at building an active iris image acquiring system which is self-adaptive to users. Two low resolution cameras are co-located in a pan-tilt-unit (PTU), for face and iris image acquisition respectively. Once the face camera detects face region in real-time video, the system controls the PTU to move towards the eye region and automatically zooms, until the iris camera captures an clear iris image for recognition. Compared with other similar works, our contribution is that we use low-resolution cameras, which can transmit image data much faster and are much cheaper than the high-resolution cameras. In the system, we use Haar-like cascaded feature to detect faces and eyes, linear transformation to predict the iris camera's position, and simple heuristic PTU control method to track eyes. A prototype device has been established, and experiments show that our system can automatically capture high-quality iris image in the range of 0.6m×0.4m×0.4m in average 3 to 5 seconds.

  9. Adaptive Optics for the 8 meter Chinese Giant Solar Telescope

    NASA Astrophysics Data System (ADS)

    Beckers, Jacques; Liu, Zhong; Deng, Yuanyong; Ji, Haisheng

    2013-12-01

    Solar ELTs enable diffraction limited imaging of the basic structure of the solar atmosphere. Magneto-hydrodynamic considerations limit their size to about 0.03 arcsec. To observe them in the near-infrared 8-meter class telescopes are needed. The Chinese Giant Solar Telescope, or CGST, is such a NIR solar ELT. It is a Ring Telescope with 8-meter outer diameter and a central clear aperture of about 6-meter diameter. At present various options for such a Gregorian type telescope are under study like a continuous ring made of segments or a multiple aperture ring made of 7 off-axis telescopes. The advantages of such a ring telescope is that its MTF covers all spatial frequencies out to those corresponding to its outer diameter, that its circular symmetry makes it polarization neutral, and that its large central hole helps thermal control and provides ample space for MCAO and Gregorian instrumentation. We present the current status of the design of the CGST. Our thinking is guided by the outstanding performance of the 1-meter vacuum solar telescope of the Yunnan Solar Observatory which like the CGST uses both AO and image reconstruction. Using it with a ring-shape aperture mask the imaging techniques for the CGST are being explored. The CGST will have Multi-Conjugate Adaptive Optics (MCAO). The peculiarities of Atmospheric Wavefront Tomography for Ring Telescopes are aided by the ample availability of guide stars on the Sun. IR MCAO-aided diffraction limited imaging offers the advantage of a large FOV, and high solar magnetic field sensitivity. Site testing is proceeding in western China, (e.g. northern Yunnan Province and Tibet). The CGST is a Chinese solar community project originated by the Yunnan Astronomical Observatory, the National Astronomical Observatories, the Purple Mountain Observatory, the Nanjing University, the Nanjing Institute of Astronomical Optics & Technology and the Beijing Normal University.

  10. Adaptive image enhancement of text images that contain touching or broken characters

    SciTech Connect

    Stubberud, P.; Kalluri, V.; Kanai, J.

    1994-11-29

    Text images that contain touching or broken characters can significantly degrade the accuracy of optical character recognition (OCR) systems. This paper proposes an adaptive image restoration technique that can improve OCR accuracy by enhancing touching or broken character images. The technique begins by processing a distorted text image with an OCR system. Using the distorted text image and output information from the OCR system, an inverse model of the distortion that caused the touching or broken character problem is generated. After generating the inverse model, the unrecognized distorted characters are filtered by the inverse model and then processes by the OCR system. To demonstrate its feasibility, six distorted text images were processed using this technique. Four of the text images, two with touching characters and two with broken characters, were synthesized using mathematical distortion models. The remaining two distorted text images, one with touching characters and one with broken characters, were distorted using a photocopier. The performance of the adaptive image restoration technique was measured using pixel accuracy and OCR improvement. The examples demonstrate that this technique can improve both the pixel and OCR accuracy of text images containing touching or broken characters.

  11. Adaptive optics applied to coherent anti-Stokes Raman scattering microscopy

    NASA Astrophysics Data System (ADS)

    Girkin, John M.; Poland, Simon P.; Wright, Amanda J.; Freudiger, Christian; Evans, Conor L.; Xie, X. Sunney

    2008-02-01

    We report on the use of adaptive optics in coherent anti-Stokes Raman scattering microscopy (CARS) to improve the image brightness and quality at increased optical penetration depths in biological material. The principle of the technique is to shape the incoming wavefront in such a way that it counteracts the aberrations introduced by imperfect optics and the varying refractive index of the sample. In recent years adaptive optics have been implemented in multiphoton and confocal microscopy. CARS microscopy is proving to be a powerful tool for non-invasive and label-free biomedical imaging with vibrational contrast. As the contrast mechanism is based on a 3 rd order non-linear optical process, it is highly susceptible to aberrations, thus CARS signals are commonly lost beyond the depth of ~100 μm in tissue. We demonstrate the combination of adaptive optics and CARS microscopy for deep-tissue imaging using a deformable membrane mirror. A random search optimization algorithm using the CARS intensity as the figure of merit determined the correct mirror-shape in order to correct for the aberrations. We highlight two different methods of implementation, using a look up table technique and by performing the optimizing in situ. We demonstrate a significant increase in brightness and image quality in an agarose/polystyrene-bead sample and white chicken muscle, pushing the penetration depth beyond 200 μm.

  12. Sensorless adaptive optics implementation in widefield optical sectioning microscopy inside in vivo Drosophila brain

    NASA Astrophysics Data System (ADS)

    Pedrazzani, Mélanie; Loriette, Vincent; Tchenio, Paul; Benrezzak, Sakina; Nutarelli, Daniele; Fragola, Alexandra

    2016-03-01

    We present an implementation of a sensorless adaptive optics loop in a widefield fluorescence microscope. This setup is designed to compensate for aberrations induced by the sample on both excitation and emission pathways. It allows fast optical sectioning inside a living Drosophila brain. We present a detailed characterization of the system performances. We prove that the gain brought to optical sectioning by realizing structured illumination microscopy with adaptive optics down to 50 μm deep inside living Drosophila brain.

  13. Adaptive thresholding of digital subtraction angiography images

    NASA Astrophysics Data System (ADS)

    Sang, Nong; Li, Heng; Peng, Weixue; Zhang, Tianxu

    2005-10-01

    In clinical practice, digital subtraction angiography (DSA) is a powerful technique for the visualization of blood vessels in the human body. Blood vessel segmentation is a main problem for 3D vascular reconstruction. In this paper, we propose a new adaptive thresholding method for the segmentation of DSA images. Each pixel of the DSA images is declared to be a vessel/background point with regard to a threshold and a few local characteristic limits depending on some information contained in the pixel neighborhood window. The size of the neighborhood window is set according to a priori knowledge of the diameter of vessels to make sure that each window contains the background definitely. Some experiments on cerebral DSA images are given, which show that our proposed method yields better results than global thresholding methods and some other local thresholding methods do.

  14. Wavefront correction and high-resolution in vivo OCT imaging with an objective integrated multi-actuator adaptive lens

    PubMed Central

    Bonora, Stefano; Jian, Yifan; Zhang, Pengfei; Zam, Azhar; Pugh, Edward N.; Zawadzki, Robert J.; Sarunic, Marinko V.

    2015-01-01

    Adaptive optics is rapidly transforming microscopy and high-resolution ophthalmic imaging. The adaptive elements commonly used to control optical wavefronts are liquid crystal spatial light modulators and deformable mirrors. We introduce a novel Multi-actuator Adaptive Lens that can correct aberrations to high order, and which has the potential to increase the spread of adaptive optics to many new applications by simplifying its integration with existing systems. Our method combines an adaptive lens with an imaged-based optimization control that allows the correction of images to the diffraction limit, and provides a reduction of hardware complexity with respect to existing state-of-the-art adaptive optics systems. The Multi-actuator Adaptive Lens design that we present can correct wavefront aberrations up to the 4th order of the Zernike polynomial characterization. The performance of the Multi-actuator Adaptive Lens is demonstrated in a wide field microscope, using a Shack-Hartmann wavefront sensor for closed loop control. The Multi-actuator Adaptive Lens and image-based wavefront-sensorless control were also integrated into the objective of a Fourier Domain Optical Coherence Tomography system for in vivo imaging of mouse retinal structures. The experimental results demonstrate that the insertion of the Multi-actuator Objective Lens can generate arbitrary wavefronts to correct aberrations down to the diffraction limit, and can be easily integrated into optical systems to improve the quality of aberrated images. PMID:26368169

  15. Wavefront correction and high-resolution in vivo OCT imaging with an objective integrated multi-actuator adaptive lens.

    PubMed

    Bonora, Stefano; Jian, Yifan; Zhang, Pengfei; Zam, Azhar; Pugh, Edward N; Zawadzki, Robert J; Sarunic, Marinko V

    2015-08-24

    Adaptive optics is rapidly transforming microscopy and high-resolution ophthalmic imaging. The adaptive elements commonly used to control optical wavefronts are liquid crystal spatial light modulators and deformable mirrors. We introduce a novel Multi-actuator Adaptive Lens that can correct aberrations to high order, and which has the potential to increase the spread of adaptive optics to many new applications by simplifying its integration with existing systems. Our method combines an adaptive lens with an imaged-based optimization control that allows the correction of images to the diffraction limit, and provides a reduction of hardware complexity with respect to existing state-of-the-art adaptive optics systems. The Multi-actuator Adaptive Lens design that we present can correct wavefront aberrations up to the 4th order of the Zernike polynomial characterization. The performance of the Multi-actuator Adaptive Lens is demonstrated in a wide field microscope, using a Shack-Hartmann wavefront sensor for closed loop control. The Multi-actuator Adaptive Lens and image-based wavefront-sensorless control were also integrated into the objective of a Fourier Domain Optical Coherence Tomography system for in vivo imaging of mouse retinal structures. The experimental results demonstrate that the insertion of the Multi-actuator Objective Lens can generate arbitrary wavefronts to correct aberrations down to the diffraction limit, and can be easily integrated into optical systems to improve the quality of aberrated images. PMID:26368169

  16. Integrin Targeting for Tumor Optical Imaging

    PubMed Central

    Ye, Yunpeng; Chen, Xiaoyuan

    2011-01-01

    Optical imaging has emerged as a powerful modality for studying molecular recognitions and molecular imaging in a noninvasive, sensitive, and real-time way. Some advantages of optical imaging include cost-effectiveness, convenience, and non-ionization safety as well as complementation with other imaging modalities such as positron emission tomography (PET), single-photon emission computed tomography (SPECT), and magnetic resonance imaging (MRI). Over the past decade, considerable advances have been made in tumor optical imaging by targeting integrin receptors in preclinical studies. This review has emphasized the construction and evaluation of diverse integrin targeting agents for optical imaging of tumors in mouse models. They mainly include some near-infrared fluorescent dye-RGD peptide conjugates, their multivalent analogs, and nanoparticle conjugates for targeting integrin αvβ3. Some compounds targeting other integrin subtypes such as α4β1 and α3 for tumor optical imaging have also been included. Both in vitro and in vivo studies have revealed some promising integrin-targeting optical agents which have further enhanced our understanding of integrin expression and targeting in cancer biology as well as related anticancer drug discovery. Especially, some integrin-targeted multifunctional optical agents including nanoparticle-based optical agents can multiplex optical imaging with other imaging modalities and targeted therapy, serving as an attractive type of theranostics for simultaneous imaging and targeted therapy. Continued efforts to discover and develop novel, innovative integrin-based optical agents with improved targeting specificity and imaging sensitivity hold great promises for improving cancer early detection, diagnosis, and targeted therapy in clinic. PMID:21546996

  17. Attenuation compensation for optical coherence tomography imaging

    NASA Astrophysics Data System (ADS)

    Chang, Shoude; Flueraru, Costel; Mao, Youxin; Sherif, Sherif

    2009-12-01

    Optical coherence tomography (OCT) is a noninvasive technique that provides micrometer-scale imaging of tissue. As most biological tissues are considered turbid, it causes attenuation of the OCT signal and limits the depth penetration. Although a few algorithms had been developed to compensate the attenuation, almost all of them need to extract the scattering parameters before doing the compensation procedure. Because the real biological samples are anisotropic and multilayer-like structure, it is not time-efficient to model and solve these scattering parameters. This paper introduces a new method to compensate the OCT signal attenuation in depth. By analyzing the input signal, a compensation function is adaptively derived for each A-scan line, which can be used effectively to compensate the energy loss in the large sections and enhance the details in the deep, dark-like areas. Three bio-samples, a piece of onion, a Poecilia Wingei fish and a piece of rabbit abdominal aorta, were used to test our method. OCT images obtained by a swept-source OCT system were processed by the proposed method. Results show the visualization of structures in OCT images has been evidently improved, especially in deep region.

  18. Adaptive optics in spinning disk microscopy: improved contrast and brightness by a simple and fast method.

    PubMed

    Fraisier, V; Clouvel, G; Jasaitis, A; Dimitrov, A; Piolot, T; Salamero, J

    2015-09-01

    Multiconfocal microscopy gives a good compromise between fast imaging and reasonable resolution. However, the low intensity of live fluorescent emitters is a major limitation to this technique. Aberrations induced by the optical setup, especially the mismatch of the refractive index and the biological sample itself, distort the point spread function and further reduce the amount of detected photons. Altogether, this leads to impaired image quality, preventing accurate analysis of molecular processes in biological samples and imaging deep in the sample. The amount of detected fluorescence can be improved with adaptive optics. Here, we used a compact adaptive optics module (adaptive optics box for sectioning optical microscopy), which was specifically designed for spinning disk confocal microscopy. The module overcomes undesired anomalies by correcting for most of the aberrations in confocal imaging. Existing aberration detection methods require prior illumination, which bleaches the sample. To avoid multiple exposures of the sample, we established an experimental model describing the depth dependence of major aberrations. This model allows us to correct for those aberrations when performing a z-stack, gradually increasing the amplitude of the correction with depth. It does not require illumination of the sample for aberration detection, thus minimizing photobleaching and phototoxicity. With this model, we improved both signal-to-background ratio and image contrast. Here, we present comparative studies on a variety of biological samples.

  19. Digital Image Watermarking via Adaptive Logo Texturization.

    PubMed

    Andalibi, Mehran; Chandler, Damon M

    2015-12-01

    Grayscale logo watermarking is a quite well-developed area of digital image watermarking which seeks to embed into the host image another smaller logo image. The key advantage of such an approach is the ability to visually analyze the extracted logo for rapid visual authentication and other visual tasks. However, logos pose new challenges for invisible watermarking applications which need to keep the watermark imperceptible within the host image while simultaneously maintaining robustness to attacks. This paper presents an algorithm for invisible grayscale logo watermarking that operates via adaptive texturization of the logo. The central idea of our approach is to recast the watermarking task into a texture similarity task. We first separate the host image into sufficiently textured and poorly textured regions. Next, for textured regions, we transform the logo into a visually similar texture via the Arnold transform and one lossless rotation; whereas for poorly textured regions, we use only a lossless rotation. The iteration for the Arnold transform and the angle of lossless rotation are determined by a model of visual texture similarity. Finally, for each region, we embed the transformed logo into that region via a standard wavelet-based embedding scheme. We employ a multistep extraction stage, in which an affine parameter estimation is first performed to compensate for possible geometrical transformations. Testing with multiple logos on a database of host images and under a variety of attacks demonstrates that the proposed algorithm yields better overall performance than competing methods.

  20. Optical spectroscopy with a near-single-mode fiber-feed and adaptive optics

    NASA Astrophysics Data System (ADS)

    Ge, Jian; Angel, J. Roger P.; Shelton, J. Christopher

    1998-07-01

    We report on first astronomical results with a cross-dispersed optical echelle spectrograph fed by a near single-mode fiber. We also present on a novel design of a new adaptive optics (AO) optimized fiber-fed cross-dispersed echelle spectrograph. The spectrograph is designed to match with AO corrected images in the optical bands provided by such as the Mt. Wilson 100 inch, Starfire Optical Range 3.5 m AO telescopes. Ultimately, it will be installed at the 6.5 m MMT, when this has high resolution AO correcting the optical spectrum. The spectrograph, fed by a 10 micron fused silica fiber, is unique in that the entire spectrum from 0.4 micron to 1.0 micron will be almost completely covered at resolution 200,000 in one exposure. The detector is a 2k X 4k AR coated back illuminated CCD with 15 micron pixel size. The close order spacing allowed by the sharp AO image makes the full cover possible. A 250 X 125 mm(superscript 2) Milton Roy R2 echelle grating with 23.2 grooves mm(superscript -1) and a blaze angle of 63.5 deg provides main dispersion. A double pass BK7 prism with 21 deg wedge angle provides cross dispersion, covering the spectrum from order 193 to 77. The spectrograph is used in the quasi- Littrow configuration with an off-axis Maksutov collimator/camera. The fiber feeds the AO corrected beams from the telescope Cassegrain focus to the spectrograph, which is set up on an optical bench. The spectrograph will be used mainly to study line profiles of solar type stars, to explore problems of indirect detection of planets and also study interstellar medium, circumstellar medium and metal abundance and isotopic ratios of extremely metal-poor stars.

  1. Design of the Subaru laser guide star adaptive optics module

    NASA Astrophysics Data System (ADS)

    Watanabe, Makoto; Takami, Hideki; Takato, Naruhisa; Colley, Stephen; Eldred, Michael; Kane, Thomas; Guyon, Olivier; Hattori, Masayuki; Goto, Miwa; Iye, Masanori; Hayano, Yutaka; Kamata, Yukiko; Arimoto, Nobuo; Kobayashi, Naoto; Minowa, Yosuke

    2004-10-01

    The laser guide star adaptive optics (AO) module for the Subaru Telescope will be installed at the f/13.9 IR Nasmyth focus, and provides the compensated image for the science instrument without change of the focal ratio. The optical components are mounted on an optical bench, and the flexure depending on the telescope pointing is eliminated. The transferred field of view for the science instrument is 2 arcmin diameter, but a 2.7 arcmin diameter field is available for tip-tilt sensing. The science path of the AO module contains five mirrors, including a pair of off-axis parabolic mirrors and a deformable mirror. It has also three additional mirrors for an image rotator. The AO module has a visible 188-element curvature based wavefront sensor (WFS) with photon-counting avalanche photodiode (APD) modules. It measures high-order terms of wavefront using either of a single laser (LGS) or natural guide star (NGS) within a 2 arcmin diameter field. The AO module has also a visible 2 x 2 sub-aperture Shack-Hartmann WFS with 16 APD modules. It measures tip-tilt and slow defocus terms of wavefront by using a single NGS within a 2.7 arcmin diameter field when a LGS is used for high-order wavefront sensing. The module has also an infrared 2 x 2 sub-aperture Shack-Hartmann WFS with a HgCdTe array as an option. Both high- and low-order visible WFSs have their own guide star acquisition units with two steering fold mirrors. The AO module has also a source simulator. It simulates LGS and NGS beams, simultaneously, with and without atmospheric turbulence by two turbulent layer at about 0 and 6 km altitudes, and reproduces the isoplanatism and the cone effect for the LGS beam.

  2. A model for radar images and its application to adaptive digital filtering of multiplicative noise.

    PubMed

    Frost, V S; Stiles, J A; Shanmugan, K S; Holtzman, J C

    1982-02-01

    Standard image processing techniques which are used to enhance noncoherent optically produced images are not applicable to radar images due to the coherent nature of the radar imaging process. A model for the radar imaging process is derived in this paper and a method for smoothing noisy radar images is also presented. The imaging model shows that the radar image is corrupted by multiplicative noise. The model leads to the functional form of an optimum (minimum MSE) filter for smoothing radar images. By using locally estimated parameter values the filter is made adaptive so that it provides minimum MSE estimates inside homogeneous areas of an image while preserving the edge structure. It is shown that the filter can be easily implemented in the spatial domain and is computationally efficient. The performance of the adaptive filter is compared (qualitatively and quantitatively) with several standard filters using real and simulated radar images.

  3. How adaptive optics may have won the Cold War

    NASA Astrophysics Data System (ADS)

    Tyson, Robert K.

    2013-05-01

    While there are many theories and studies concerning the end of the Cold War, circa 1990, I postulate that one of the contributors to the result was the development of adaptive optics. The emergence of directed energy weapons, specifically space-based and ground-based high energy lasers made practicable with adaptive optics, showed that a successful defense against inter-continental ballistic missiles was not only possible, but achievable in a reasonable period of time.

  4. Astronomy Applications of Adaptive Optics at Lawrence Livermore National Laboratory

    SciTech Connect

    Bauman, B J; Gavel, D T

    2003-04-23

    Astronomical applications of adaptive optics at Lawrence Livermore National Laboratory (LLNL) has a history that extends from 1984. The program started with the Lick Observatory Adaptive Optics system and has progressed through the years to lever-larger telescopes: Keck, and now the proposed CELT (California Extremely Large Telescope) 30m telescope. LLNL AO continues to be at the forefront of AO development and science.

  5. Astronomy applications of adaptive optics at Lawrence Livermore National Laboratory

    NASA Astrophysics Data System (ADS)

    Bauman, Brian J.; Gavel, Donald T.

    2003-06-01

    Astronomical applications of adaptive optics at Lawrence Livermore National Laboratory (LLNL) has a history that extends from 1984. The program started with the Lick Observatory Adaptive Optics system and has progressed through the years to lever-larger telescopes: Keck, and now the proposed CELT (California Extremely Large Telescope) 30m telescope. LLNL AO continues to be at the forefront of AO development and science.

  6. Edge Detection to Isolate Motion in Adaptive Optics Systems

    SciTech Connect

    Chan, C W

    2003-07-11

    Adaptive optics uses signal processing techniques and deformable mirrors to minimize image degradation caused by phase aberrations. In the case of telescope imaging, the atmosphere causes phase aberrations. In the case of satellite imaging, errors due to the ultra-light-weight characteristics of the primary mirror cause phase aberrations. Scene-based Shack-Hartmann Wave Front Sensing takes the correlation between successive wavelets to determine these phase aberrations. A large problem with the scene-based approach is that motion, such as a moving car, can cause the correlation of two lenslets to peak, not where the scenes align, but where the moving object in each frame aligns. As such, the continued use of scene-based Wave Front Sensing necessitates successful isolation of moving objects from a stationary background scene. With the knowledge of which pixels are immobile, one should avoid the problem of locking onto a moving object when taking the correlation of two successive frames in time. Two main requirements of isolation are consistency and efficiency. In this document I will discuss the different edge detection algorithms explored for moving object isolation and how I came to the conclusion that, for our purposes of scene-based Shack-Hartmann WFS, edge detection is too inconsistent to be of any use. Because the Shack-Hartmann lenslets limits us to low resolutions, edge detection that works on higher resolution images will not work on our images. The results of each algorithm will show that with so few pixels per subaperature, edge detection is a poor method of identifying moving objects.

  7. Design architectures for optically multiplexed imaging.

    PubMed

    Shepard, R Hamilton; Rachlin, Yaron; Shah, Vinay; Shih, Tina

    2015-11-30

    Optically multiplexed imaging is the process by which multiple images are overlaid on a single image surface. Uniquely encoding the discrete images allows scene reconstruction from multiplexed images via post processing. We describe a class of optical systems that can achieve high density image multiplexing through a novel division of aperture technique. Fundamental design considerations and performance attributes for this sensor architecture are discussed. A number of spatial and temporal encoding methods are presented including point spread function engineering, amplitude modulation, and image shifting. Results from a prototype five-channel sensor are presented using three different encoding methods in sparse-scene star tracking demonstration. A six-channel optically multiplexed prototype sensor is used to reconstruct imagery from information rich dense scenes through dynamic image shifting. PMID:26698767

  8. Performance of the Gemini Planet Imager’s adaptive optics system

    DOE PAGES

    Poyneer, Lisa A.; Palmer, David W.; Macintosh, Bruce; Savransky, Dmitry; Sadakuni, Naru; Thomas, Sandrine; Véran, Jean-Pierre; Follette, Katherine B.; Greenbaum, Alexandra Z.; Mark Ammons, S.; et al

    2016-01-07

    The Gemini Planet Imager’s adaptive optics (AO) subsystem was designed specifically to facilitate high-contrast imaging. We give a definitive description of the system’s algorithms and technologies as built. Ultimately, the error budget indicates that for all targets and atmospheric conditions AO bandwidth error is the largest term.

  9. GPU-based computational adaptive optics for volumetric optical coherence microscopy

    NASA Astrophysics Data System (ADS)

    Tang, Han; Mulligan, Jeffrey A.; Untracht, Gavrielle R.; Zhang, Xihao; Adie, Steven G.

    2016-03-01

    Optical coherence tomography (OCT) is a non-invasive imaging technique that measures reflectance from within biological tissues. Current higher-NA optical coherence microscopy (OCM) technologies with near cellular resolution have limitations on volumetric imaging capabilities due to the trade-offs between resolution vs. depth-of-field and sensitivity to aberrations. Such trade-offs can be addressed using computational adaptive optics (CAO), which corrects aberration computationally for all depths based on the complex optical field measured by OCT. However, due to the large size of datasets plus the computational complexity of CAO and OCT algorithms, it is a challenge to achieve high-resolution 3D-OCM reconstructions at speeds suitable for clinical and research OCM imaging. In recent years, real-time OCT reconstruction incorporating both dispersion and defocus correction has been achieved through parallel computing on graphics processing units (GPUs). We add to these methods by implementing depth-dependent aberration correction for volumetric OCM using plane-by-plane phase deconvolution. Following both defocus and aberration correction, our reconstruction algorithm achieved depth-independent transverse resolution of 2.8 um, equal to the diffraction-limited focal plane resolution. We have translated the CAO algorithm to a CUDA code implementation and tested the speed of the software in real-time using two GPUs - NVIDIA Quadro K600 and Geforce TITAN Z. For a data volume containing 4096×256×256 voxels, our system's processing speed can keep up with the 60 kHz acquisition rate of the line-scan camera, and takes 1.09 seconds to simultaneously update the CAO correction for 3 en face planes at user-selectable depths.

  10. An adaptive optic for correcting low-order wavefront aberrations

    SciTech Connect

    Thompson, C A; Wilhelmsen, J

    1999-09-02

    Adaptive Optics used for correcting low-order wavefront aberrations were tested and compared using interferometry, beam propagation, and a far-field test. Results confirm that the design and manufacturing specifications were met. Experimental data also confirms theoretical performance expectations, indicating the usefulness of these optics (especially in a laser-beam processing system), and identifying the resulting differences between the two fabrication methods used to make the optics.

  11. Optical design trade-offs of the multi conjugate adaptive optics relay for the European Extremely Large Telescope

    NASA Astrophysics Data System (ADS)

    Lombini, Matteo; Diolaiti, Emiliano; De Rosa, Adriano

    2014-08-01

    The scope of this paper is to describe some possible design concepts of the post optical relay inside the multi conjugate adaptive optics module for the European Extremely Large Telescope. The module is planned to be placed at the Nasmyth focus of the telescope. The optical relay must re-image the telescope focal plane with diffraction limited performance and low geometric distortion, for a field of view of 75" and for a wavelength range between 0.8 and 2.4μm. A technical annular field of view with inner diameter of 75" and outer diameter of 160" to search 3 for natural guide stars is also required. Wavefront sensing is performed by means of 6 laser guide stars arranged on a circle of at least 120" diameter while wavefront correction is performed by two deformable mirrors inside the relay, in addition to the telescope adaptive mirror. The final optical design will be a trade-off among adaptive optics performance, optical interface requirements, mechanical interface requirements and technological feasibility of key hardware components. The size of the deformable mirrors and the image quality of the layer conjugates are important design drivers, related to the design of the collimating optics after the input focal plane and to the deformable mirrors tilt respect to the chief ray. The optical interface at the output focal plane must be acceptable for the client instruments, in terms of field curvature, focal ratio and exit pupil position. The number of optical surfaces inside the relay has to be as small as possible to limit thermal background. Splitting of the laser guide star channel from the science light channel may be achieved either in wavelength, by means of a dichroic placed close to a pupil image, or in field, by means of an perforated dichroic placed at an intermediate focal plane. The laser guide star beams have to be focused with acceptable optical performance on a fixed image plane compensating the effects of the sodium layer range variation with Zenith

  12. Modelling MEMS deformable mirrors for astronomical adaptive optics

    NASA Astrophysics Data System (ADS)

    Blain, Celia

    As of July 2012, 777 exoplanets have been discovered utilizing mainly indirect detection techniques. The direct imaging of exoplanets is the next goal for astronomers, because it will reveal the diversity of planets and planetary systems, and will give access to the exoplanet's chemical composition via spectroscopy. With this spectroscopic knowledge, astronomers will be able to know, if a planet is terrestrial and, possibly, even find evidence of life. With so much potential, this branch of astronomy has also captivated the general public attention. The direct imaging of exoplanets remains a challenging task, due to (i) the extremely high contrast between the parent star and the orbiting exoplanet and (ii) their small angular separation. For ground-based observatories, this task is made even more difficult, due to the presence of atmospheric turbulence. High Contrast Imaging (HCI) instruments have been designed to meet this challenge. HCI instruments are usually composed of a coronagraph coupled with the full onaxis corrective capability of an Extreme Adaptive Optics (ExAO) system. An efficient coronagraph separates the faint planet's light from the much brighter starlight, but the dynamic boiling speckles, created by the stellar image, make exoplanet detection impossible without the help of a wavefront correction device. The Subaru Coronagraphic Extreme Adaptive Optics (SCExAO) system is a high performance HCI instrument developed at Subaru Telescope. The wavefront control system of SCExAO consists of three wavefront sensors (WFS) coupled with a 1024- actuator Micro-Electro-Mechanical-System (MEMS) deformable mirror (DM). MEMS DMs offer a large actuator density, allowing high count DMs to be deployed in small size beams. Therefore, MEMS DMs are an attractive technology for Adaptive Optics (AO) systems and are particularly well suited for HCI instruments employing ExAO technologies. SCExAO uses coherent light modulation in the focal plane introduced by the DM, for

  13. Optical design for LED dental lighting with imaging optic technique

    NASA Astrophysics Data System (ADS)

    Kwon, Young-Hoon; Bae, Seung-Chul; Lim, Hae-Ryong; Jang, Ja-Soon

    2011-10-01

    We did a research as follows. First of all, selected optimum LEDs and mixed it for higher CRI, target CCT and illuminance. The following step is optical module design. Light directional characteristics of dental lighting must be concentrated to illuminate a part. Because This part is oral cavity, The feature of illumination pattern is rectangular. For uniformity of illuminance and clearer pattern boundary at reference distance, we designed it as direct type (no use reflector) by imaging optic technique. First, Image is rectangular feature, so object must be the same feature with magnification in general imaging optics. But the emitting surface feature of LED (1W grade) is square or circular generally. For that reason, made object as rectangular source with rectangular lightguide. This optical component was designed for higher efficiency by illumination optic technique. Next, we designed optical lenses based on imaging optic technique for image object feature using Code V. set to high NA for light efficiency in this design. Fundamentally, Finally, This product is luminaire so illumination simulation and result analysis were executed by LightTools as illumination design software.

  14. Compact optical imaging system for arrays of optical thyristors.

    PubMed

    Kirk, A; Goulet, A; Thienpont, H; McArdle, N; Brenner, K H; Kuijk, M; Heremans, P; Veretennicoff, I

    1997-05-10

    A compact and modular optical system that employs gradient-refractive-index rod lenses to image arrays of Lambertian sources is characterized both experimentally and by ray-tracing simulations. A hybrid optical system that incorporates additional microlens arrays to reduce transmittance losses and aberrations is also modeled, and the two systems are compared.

  15. Deep and Clear Optical Imaging of Thick Inhomogeneous Samples

    PubMed Central

    Andilla, Jordi; Maandhui, Amina; Frongia, Céline; Lobjois, Valérie; Ducommun, Bernard; Lorenzo, Corinne

    2012-01-01

    Inhomogeneity in thick biological specimens results in poor imaging by light microscopy, which deteriorates as the focal plane moves deeper into the specimen. Here, we have combined selective plane illumination microscopy (SPIM) with wavefront sensor adaptive optics (wao). Our waoSPIM is based on a direct wavefront measure using a Hartmann-Shack wavefront sensor and fluorescent beads as point source emitters. We demonstrate the use of this waoSPIM method to correct distortions in three-dimensional biological imaging and to improve the quality of images from deep within thick inhomogeneous samples. PMID:22558226

  16. Constraints on Extrasolar Planet Populations from VLT NACO/SDI and MMT SDI and Direct Adaptive Optics Imaging Surveys: Giant Planets are Rare at Large Separations

    NASA Astrophysics Data System (ADS)

    Nielsen, E. L.; Close, L. M.; Biller, B. A.; Masciadri, E.; Lenzen, R.

    2010-01-01

    We examine the implications for the distribution of extrasolar planets based on the null results from two of the largest direct imaging surveys published to date. Combining the measured contrast curves from Masciadri et al. (2005) and Biller et al. (2007), we consider what distributions of planet masses and semi-major axes can be ruled out by these data, based on Monte Carlo simulations of planet populations. We can set the following upper limit with 95% confidence: the fraction of stars with planets with semi-major axis between 20 and 100 AU, and mass above 4 MJup, is 20% or less. Also, with a distribution of planet mass of {dN}/{dM} ∝ M-1.16 in the range of 0.5-13 M Jup , we can rule out a power-law distribution for semi-major axis ({dN}/{da} ∝ aα) with index 0 and upper cut-off of 18 AU, and index -0.5 with an upper cut-off of 48 AU. For the distribution suggested by Cumming et al. (2008), a power-law of index -0.61, we can place an upper limit of 75 AU on the semi-major axis distribution.

  17. Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes

    PubMed Central

    Wang, Kai; Milkie, Dan; Saxena, Ankur; Engerer, Peter; Misgeld, Thomas; Bronner, Marianne E.; Mumm, Jeff; Betzig, Eric

    2014-01-01

    Using a de-scanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture and a 14 ms update rate. This permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens, and recover diffraction-limited imaging over large (> 240 μm)3 volumes. We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain. PMID:24727653

  18. Horizontal Path Laser Communications Employing MEMS Adaptive Optics Correction

    SciTech Connect

    Thompson, C A; Wilks, S C; Brase, J M; Young, R A; Johnson, G W; Ruggiero, A J

    2001-09-05

    Horizontal path laser communications are beginning to provide attractive alternatives for high-speed optical communications, In particular, companies are beginning to sell fiberless alternatives for intranet and sporting event video. These applications are primarily aimed at short distance applications (on the order of 1 km pathlength). There exists a potential need to extend this pathlength to distances much greater than a 1km. For cases of long distance optical propagation, atmospheric turbulence will ultimately limit the maximum achievable data rate. In this paper, we propose a method of improved signal quality through the use of adaptive optics. In particular, we show work in progress toward a high-speed, small footprint Adaptive Optics system for horizontal path laser communications. Such a system relies heavily on recent progress in Micro-Electro-Mechanical Systems (MEMS) deformable mirrors as well as improved communication and computational components. In this paper we detail two Adaptive Optics approaches for improved through-put, the first is the compensated receiver (the traditional Adaptive Optics approach), the second is the compensated transmitter/receiver. The second approach allows for correction of the optical wavefront before transmission from the transmitter and prior to detection at the receiver.

  19. Pencil beam all-optical ultrasound imaging

    PubMed Central

    Alles, Erwin J.; Noimark, Sacha; Zhang, Edward; Beard, Paul C.; Desjardins, Adrien E.

    2016-01-01

    A miniature, directional fibre-optic acoustic source is presented that employs geometrical focussing to generate a nearly-collimated acoustic pencil beam. When paired with a fibre-optic acoustic detector, an all-optical ultrasound probe with an outer diameter of 2.5 mm is obtained that acquires a pulse-echo image line at each probe position without the need for image reconstruction. B-mode images can be acquired by translating the probe and concatenating the image lines, and artefacts resulting from probe positioning uncertainty are shown to be significantly lower than those observed for conventional synthetic aperture scanning of a non-directional acoustic source. The high image quality obtained for excised vascular tissue suggests that the all-optical ultrasound probe is ideally suited for in vivo, interventional applications. PMID:27699130

  20. Pencil beam all-optical ultrasound imaging

    PubMed Central

    Alles, Erwin J.; Noimark, Sacha; Zhang, Edward; Beard, Paul C.; Desjardins, Adrien E.

    2016-01-01

    A miniature, directional fibre-optic acoustic source is presented that employs geometrical focussing to generate a nearly-collimated acoustic pencil beam. When paired with a fibre-optic acoustic detector, an all-optical ultrasound probe with an outer diameter of 2.5 mm is obtained that acquires a pulse-echo image line at each probe position without the need for image reconstruction. B-mode images can be acquired by translating the probe and concatenating the image lines, and artefacts resulting from probe positioning uncertainty are shown to be significantly lower than those observed for conventional synthetic aperture scanning of a non-directional acoustic source. The high image quality obtained for excised vascular tissue suggests that the all-optical ultrasound probe is ideally suited for in vivo, interventional applications.

  1. Adaptive-optic approach to mitigating aero-optic disturbances for a forced shear layer

    NASA Astrophysics Data System (ADS)

    Nightingale, Alice M.

    Non-uniform, variable-density fields, resulting from compressibility effects in turbulent flows, are the source of aero-optical distortions which cause significant reductions in optical system performance. As a laser beam transverses through an optically active medium, containing index-of-refraction variations, several optical phenomena occur including beam wander, image distortion, and beam defocus. When encountering a variation in the index field, light waves refract causing an otherwise planar wavefront of a laser beam to become aberrated, contributing to the adverse effects mentioned above. Adaptive-Optics (AO) is a technique used to correct for such spatially and temporally varying aberrations on an optical beam by applying a conjugate waveform correction prior to the beams transmission through the flow. Conventional AO systems are bandwidth limited by real-time processing issues and wavefront sensor limitations. Therefore, an alternative to the conventional AO approach has been proposed, developed and evaluated with the goal of overcoming such bandwidth limitations. The alternative AO system, presented throughout this document, consists of two main features; feed-forward flow control and a phase-locked-loop AO control strategy. Initially irregular, unpredictable large-scale structures within a shear layer are regularized using flow control. Subsequently, the resulting optical wavefront, and corresponding optical signal, emerging from the regularized flow becomes more periodic and predictable effectively reducing the bandwidth necessary to make real-time corrections. A phase-lock-loop controller is then used to perform real-time corrections. Wavefront corrections are estimated based upon the regularized flow, while two small aperture laser beams provide a non-intrusive means of acquiring amplitude and phase error measurements. The phase-lock-loop controller uses these signals as feedback to synchronize the deformable mirror's waveform to that of the shear

  2. NAOMI: nanoparticle-assisted optical molecular imaging

    NASA Astrophysics Data System (ADS)

    Faber, Dirk J.; de Bruin, Martijn; Aalders, Maurice C. G.; Verbraak, Frank D.; van Leeuwen, Ton G.

    2007-02-01

    We present our first steps towards nanoparticle assisted, optical molecular imaging (NAOMI) using biodegradable nanoparticles. Our focus is on using optical coherence tomography(OCT) as the imaging modality. We propose to use nanoparticles based on biodegradable polymers, loaded with carefully selected dyes as contrast agent, and outline a method for establishing their desired optical properties prior to synthesis. Moreover, we perform a qualitative pilot study using these biodegradable nanoparticles, measuring their optical properties which are found to be in line with theoretical predictions.

  3. An adaptive extended Kalman filter for fluorescence diffuse optical tomography of tumor pharmacokinetics

    NASA Astrophysics Data System (ADS)

    Wang, Xin; Wu, Linhui; Yi, Xi; Zhang, Limin; Gao, Feng; Zhao, Huijuan

    2014-03-01

    According to the morphological differences in the vascularization between healthy and diseased tissues, pharmacokinetic-rate images of fluorophore can provide diagnostic information for tumor differentiation, and especially have the potential for staging of tumors. In this paper, fluorescence diffuse optical tomography method is firstly used to acquire metabolism-related time-course images of the fluorophore concentration. Based on a two-compartment model comprised of plasma and extracelluar-extravascular space, we next propose an adaptive-EKF framework to estimate the pharmacokinetic-rate images. With the aid of a forgetting factor, the adaptive-EKF compensate the inaccuracy initial values and emphasize the effect of the current data in order to realize a better online estimation compared with the conventional EKF. We use simulate data to evaluate the performance of the proposed methodology. The results suggest that the adaptive-EKF can obtain preferable pharmacokinetic-rate images than the conventional EKF with higher quantitativeness and noise robustness.

  4. Optical design for the Narrow Field InfraRed Adaptive Optics System (NFIRAOS) Petite on the Thirty Meter Telescope

    SciTech Connect

    Bauman, B; Gavel, D; Dekany, R; Ellerbroek, B

    2005-08-02

    We describe an exploratory optical design for the Narrow Field InfraRed Adaptive Optics (AO) System (NFIRAOS) Petite, a proposed adaptive optics system for the Thirty Meter Telescope Project. NFIRAOS will feed infrared spectrograph and wide-field imaging instruments with a diffraction limited beam. The adaptive optics system will require multi-guidestar tomographic wavefront sensing and multi-conjugate AO correction. The NFIRAOS Petite design specifications include two small 60 mm diameter deformable mirrors (DM's) used in a woofer/tweeter or multiconjugate arrangement. At least one DM would be a micro-electromechanical system (MEMS) DM. The AO system would correct a 10 to 30 arcsec diameter science field as well as laser guide stars (LGS's) located within a 60 arcsec diameter field and low-order or tip/tilt natural guide stars (NGS's) within a 60 arcsec diameter field. The WFS's are located downstream of the DM's so that they can be operated in true closed-loop, which is not necessarily a given in extremely large telescope adaptive optics design. The WFS's include adjustable corrector elements which correct the static aberrations of the AO relay due to field position and LGS distance height.

  5. Coded Access Optical Sensor (CAOS) Imager

    NASA Astrophysics Data System (ADS)

    Riza, N. A.; Amin, M. J.; La Torre, J. P.

    2015-04-01

    High spatial resolution, low inter-pixel crosstalk, high signal-to-noise ratio (SNR), adequate application dependent speed, economical and energy efficient design are common goals sought after for optical image sensors. In optical microscopy, overcoming the diffraction limit in spatial resolution has been achieved using materials chemistry, optimal wavelengths, precision optics and nanomotion-mechanics for pixel-by-pixel scanning. Imagers based on pixelated imaging devices such as CCD/CMOS sensors avoid pixel-by-pixel scanning as all sensor pixels operate in parallel, but these imagers are fundamentally limited by inter-pixel crosstalk, in particular with interspersed bright and dim light zones. In this paper, we propose an agile pixel imager sensor design platform called Coded Access Optical Sensor (CAOS) that can greatly alleviate the mentioned fundamental limitations, empowering smart optical imaging for particular environments. Specifically, this novel CAOS imager engages an application dependent electronically programmable agile pixel platform using hybrid space-time-frequency coded multiple-access of the sampled optical irradiance map. We demonstrate the foundational working principles of the first experimental electronically programmable CAOS imager using hybrid time-frequency multiple access sampling of a known high contrast laser beam irradiance test map, with the CAOS instrument based on a Texas Instruments (TI) Digital Micromirror Device (DMD). This CAOS instrument provides imaging data that exhibits 77 dB electrical SNR and the measured laser beam image irradiance specifications closely match (i.e., within 0.75% error) the laser manufacturer provided beam image irradiance radius numbers. The proposed CAOS imager can be deployed in many scientific and non-scientific applications where pixel agility via electronic programmability can pull out desired features in an irradiance map subject to the CAOS imaging operation.

  6. Extreme Adaptive Optics Testbed: Performance and Characterization of a 1024 Deformable Mirror

    SciTech Connect

    Evans, J W; Morzinski, K; Severson, S; Poyneer, L; Macintosh, B; Dillon, D; REza, L; Gavel, D; Palmer, D

    2005-10-30

    We have demonstrated that a microelectrical mechanical systems (MEMS) deformable mirror can be flattened to < 1 nm RMS within controllable spatial frequencies over a 9.2-mm aperture making it a viable option for high-contrast adaptive optics systems (also known as Extreme Adaptive Optics). The Extreme Adaptive Optics Testbed at UC Santa Cruz is being used to investigate and develop technologies for high-contrast imaging, especially wavefront control. A phase shifting diffraction interferometer (PSDI) measures wavefront errors with sub-nm precision and accuracy for metrology and wavefront control. Consistent flattening, required testing and characterization of the individual actuator response, including the effects of dead and low-response actuators. Stability and repeatability of the MEMS devices was also tested. An error budget for MEMS closed loop performance will summarize MEMS characterization.

  7. Robust image registration using adaptive coherent point drift method

    NASA Astrophysics Data System (ADS)

    Yang, Lijuan; Tian, Zheng; Zhao, Wei; Wen, Jinhuan; Yan, Weidong

    2016-04-01

    Coherent point drift (CPD) method is a powerful registration tool under the framework of the Gaussian mixture model (GMM). However, the global spatial structure of point sets is considered only without other forms of additional attribute information. The equivalent simplification of mixing parameters and the manual setting of the weight parameter in GMM make the CPD method less robust to outlier and have less flexibility. An adaptive CPD method is proposed to automatically determine the mixing parameters by embedding the local attribute information of features into the construction of GMM. In addition, the weight parameter is treated as an unknown parameter and automatically determined in the expectation-maximization algorithm. In image registration applications, the block-divided salient image disk extraction method is designed to detect sparse salient image features and local self-similarity is used as attribute information to describe the local neighborhood structure of each feature. The experimental results on optical images and remote sensing images show that the proposed method can significantly improve the matching performance.

  8. Magnetic resonance imaging of optic nerve

    PubMed Central

    Gala, Foram

    2015-01-01

    Optic nerves are the second pair of cranial nerves and are unique as they represent an extension of the central nervous system. Apart from clinical and ophthalmoscopic evaluation, imaging, especially magnetic resonance imaging (MRI), plays an important role in the complete evaluation of optic nerve and the entire visual pathway. In this pictorial essay, the authors describe segmental anatomy of the optic nerve and review the imaging findings of various conditions affecting the optic nerves. MRI allows excellent depiction of the intricate anatomy of optic nerves due to its excellent soft tissue contrast without exposure to ionizing radiation, better delineation of the entire visual pathway, and accurate evaluation of associated intracranial pathologies. PMID:26752822

  9. Space-based optical image encryption.

    PubMed

    Chen, Wen; Chen, Xudong

    2010-12-20

    In this paper, we propose a new method based on a three-dimensional (3D) space-based strategy for the optical image encryption. The two-dimensional (2D) processing of a plaintext in the conventional optical encryption methods is extended to a 3D space-based processing. Each pixel of the plaintext is considered as one particle in the proposed space-based optical image encryption, and the diffraction of all particles forms an object wave in the phase-shifting digital holography. The effectiveness and advantages of the proposed method are demonstrated by numerical results. The proposed method can provide a new optical encryption strategy instead of the conventional 2D processing, and may open up a new research perspective for the optical image encryption.

  10. Background Noise Mitigation in Deep-Space Optical Communications Using Adaptive Optics

    NASA Astrophysics Data System (ADS)

    Lee, S.; Wilson, K. E.; Troy, M.

    2005-05-01

    Over the last decade, adaptive optics technology has moved from the purview of a Department of Defense laboratory to astronomical telescopes around the world, and recently to industry, where adaptive optics systems have been developed to correct atmospheric-induced signal fades on high-bandwidth horizontal-path optical links. As JPL develops optical communications technology for high-bandwidth optical links from its deep-space probes, we are exploring the application of adaptive optics to the optical deep-space receiver to improve the quality of the link under turbulent atmospheric and high-background conditions. To provide maximum communications support, the operational deep-space optical communications receiver will need to point close to the Sun or to a bright Sun-illuminated planet. Under these conditions, the background noise from the sky degrades the quality of the optical link, especially when the atmospheric seeing is poor. In this work, we analyze how adaptive optics could be used to mitigate the effects of sky and planetary background noise on the deep-space optical communications receiver's performance in poor seeing conditions. Our results show that, under nominal background sky conditions, gains of 4 dB can be achieved for the uncoded bit-error rate of 0.01.

  11. Progress in Evaluating Quantitative Optical Gas Imaging

    EPA Science Inventory

    Development of advanced fugitive emission detection and assessment technologies that facilitate cost effective leak and malfunction mitigation strategies is an ongoing goal shared by industry, regulators, and environmental groups. Optical gas imaging (OGI) represents an importan...

  12. Reverse-Time Migration Based Optical Imaging.

    PubMed

    Wang, Zhiyong; Ding, Hao; Lu, Guijin; Bi, Xiaohong

    2016-01-01

    We theoretically demonstrated a new optical imaging technique based on reverse-time migration (RTM) for reconstructing optical structures in homogeneous media for the first time. RTM is a powerful wave-equation-based method to reconstruct the image of the structure by modeling the wave propagation inside the media with both forward modeling and reverse-time extrapolation. While RTM is commonly used with acoustic seismic waves, this paper represents the first effort to develop optical RTM imaging method for biomedical research. To refine the image quality, we further developed new methods to suppress the low-wavenumber artifact (LWA). When compared with the conventional means for LWA suppression such as Laplacian filtering, illumination normalization, and the ratio method, our new derivative-based and power-image methods are able to significantly reduce LWA, resulting in high-quality reconstructed images with sufficient contrasts and spatial resolutions for structure identification. The optical RTM imaging technique may provide a new platform for non-invasive optical imaging of structures in deep layers of tissues for biomedical applications. PMID:26292337

  13. Reverse-Time Migration Based Optical Imaging.

    PubMed

    Wang, Zhiyong; Ding, Hao; Lu, Guijin; Bi, Xiaohong

    2016-01-01

    We theoretically demonstrated a new optical imaging technique based on reverse-time migration (RTM) for reconstructing optical structures in homogeneous media for the first time. RTM is a powerful wave-equation-based method to reconstruct the image of the structure by modeling the wave propagation inside the media with both forward modeling and reverse-time extrapolation. While RTM is commonly used with acoustic seismic waves, this paper represents the first effort to develop optical RTM imaging method for biomedical research. To refine the image quality, we further developed new methods to suppress the low-wavenumber artifact (LWA). When compared with the conventional means for LWA suppression such as Laplacian filtering, illumination normalization, and the ratio method, our new derivative-based and power-image methods are able to significantly reduce LWA, resulting in high-quality reconstructed images with sufficient contrasts and spatial resolutions for structure identification. The optical RTM imaging technique may provide a new platform for non-invasive optical imaging of structures in deep layers of tissues for biomedical applications.

  14. Optical image encryption using multilevel Arnold transform and noninterferometric imaging

    NASA Astrophysics Data System (ADS)

    Chen, Wen; Chen, Xudong

    2011-11-01

    Information security has attracted much current attention due to the rapid development of modern technologies, such as computer and internet. We propose a novel method for optical image encryption using multilevel Arnold transform and rotatable-phase-mask noninterferometric imaging. An optical image encryption scheme is developed in the gyrator transform domain, and one phase-only mask (i.e., phase grating) is rotated and updated during image encryption. For the decryption, an iterative retrieval algorithm is proposed to extract high-quality plaintexts. Conventional encoding methods (such as digital holography) have been proven vulnerably to the attacks, and the proposed optical encoding scheme can effectively eliminate security deficiency and significantly enhance cryptosystem security. The proposed strategy based on the rotatable phase-only mask can provide a new alternative for data/image encryption in the noninterferometric imaging.

  15. Optical magnetic imaging of living cells

    PubMed Central

    Le Sage, D.; Arai, K.; Glenn, D. R.; DeVience, S. J.; Pham, L. M.; Rahn-Lee, L.; Lukin, M. D.; Yacoby, A.; Komeili, A.; Walsworth, R. L.

    2013-01-01

    Magnetic imaging is a powerful tool for probing biological and physical systems. However, existing techniques either have poor spatial resolution compared to optical microscopy and are hence not generally applicable to imaging of sub-cellular structure (e.g., magnetic resonance imaging [MRI]1), or entail operating conditions that preclude application to living biological samples while providing sub-micron resolution (e.g., scanning superconducting quantum interference device [SQUID] microscopy2, electron holography3, and magnetic resonance force microscopy [MRFM]4). Here we demonstrate magnetic imaging of living cells (magnetotactic bacteria) under ambient laboratory conditions and with sub-cellular spatial resolution (400 nm), using an optically-detected magnetic field imaging array consisting of a nanoscale layer of nitrogen-vacancy (NV) colour centres implanted at the surface of a diamond chip. With the bacteria placed on the diamond surface, we optically probe the NV quantum spin states and rapidly reconstruct images of the vector components of the magnetic field created by chains of magnetic nanoparticles (magnetosomes) produced in the bacteria, and spatially correlate these magnetic field maps with optical images acquired in the same apparatus. Wide-field sCMOS acquisition allows parallel optical and magnetic imaging of multiple cells in a population with sub-micron resolution and >100 micron field-of-view. Scanning electron microscope (SEM) images of the bacteria confirm that the correlated optical and magnetic images can be used to locate and characterize the magnetosomes in each bacterium. The results provide a new capability for imaging bio-magnetic structures in living cells under ambient conditions with high spatial resolution, and will enable the mapping of a wide range of magnetic signals within cells and cellular networks5, 6. PMID:23619694

  16. Beaconless adaptive-optics technique for HEL beam control

    NASA Astrophysics Data System (ADS)

    Khizhnyak, Anatoliy; Markov, Vladimir

    2016-05-01

    Effective performance of forthcoming laser systems capable of power delivery on a distant target requires an adaptive optics system to correct atmospheric perturbations on the laser beam. The turbulence-induced effects are responsible for beam wobbling, wandering, and intensity scintillation, resulting in degradation of the beam quality and power density on the target. Adaptive optics methods are used to compensate for these negative effects. In its turn, operation of the AOS system requires a reference wave that can be generated by the beacon on the target. This report discusses a beaconless approach for wavefront correction with its performance based on the detection of the target-scattered light. Postprocessing of the beacon-generated light field enables retrieval and detailed characterization of the turbulence-perturbed wavefront -data that is essential to control the adaptive optics module of a high-power laser system.

  17. Magellan Adaptive Optics First-light Observations of the Exoplanet β Pic b. II. 3-5 μm Direct Imaging with MagAO+Clio, and the Empirical Bolometric Luminosity of a Self-luminous Giant Planet

    NASA Astrophysics Data System (ADS)

    Morzinski, Katie M.; Males, Jared R.; Skemer, Andy J.; Close, Laird M.; Hinz, Phil M.; Rodigas, T. J.; Puglisi, Alfio; Esposito, Simone; Riccardi, Armando; Pinna, Enrico; Xompero, Marco; Briguglio, Runa; Bailey, Vanessa P.; Follette, Katherine B.; Kopon, Derek; Weinberger, Alycia J.; Wu, Ya-Lin

    2015-12-01

    Young giant exoplanets are a unique laboratory for understanding cool, low-gravity atmospheres. A quintessential example is the massive extrasolar planet β Pic b, which is 9 AU from and embedded in the debris disk of the young nearby A6V star β Pictoris. We observed the system with first light of the Magellan Adaptive Optics (MagAO) system. In Paper I we presented the first CCD detection of this planet with MagAO+VisAO. Here we present four MagAO+Clio images of β Pic b at 3.1 μm, 3.3 μm, L‧, and {M}\\prime , including the first observation in the fundamental CH4 band. To remove systematic errors from the spectral energy distribution (SED), we re-calibrate the literature photometry and combine it with our own data, for a total of 22 independent measurements at 16 passbands from 0.99 to 4.8 μm. Atmosphere models demonstrate the planet is cloudy but are degenerate in effective temperature and radius. The measured SED now covers >80% of the planet’s energy, so we approach the bolometric luminosity empirically. We calculate the luminosity by extending the measured SED with a blackbody and integrating to find log({{L}}{bol}/{{L}}⊙ ) \\=\\-3.78+/- 0.03. From our bolometric luminosity and an age of 23 ± 3 Myr, hot-start evolutionary tracks give a mass of 12.7 ± 0.3 {{M}}{Jup}, radius of 1.45 ± 0.02 {{R}}{Jup}, and Teff of 1708 ± 23 K (model-dependent errors not included). Our empirically determined luminosity is in agreement with values from atmospheric models (typically -3.8 dex), but brighter than values from the field-dwarf bolometric correction (typically -3.9 dex), illustrating the limitations in comparing young exoplanets to old brown dwarfs.

  18. Advanced Imaging Optics Utilizing Wavefront Coding.

    SciTech Connect

    Scrymgeour, David; Boye, Robert; Adelsberger, Kathleen

    2015-06-01

    Image processing offers a potential to simplify an optical system by shifting some of the imaging burden from lenses to the more cost effective electronics. Wavefront coding using a cubic phase plate combined with image processing can extend the system's depth of focus, reducing many of the focus-related aberrations as well as material related chromatic aberrations. However, the optimal design process and physical limitations of wavefront coding systems with respect to first-order optical parameters and noise are not well documented. We examined image quality of simulated and experimental wavefront coded images before and after reconstruction in the presence of noise. Challenges in the implementation of cubic phase in an optical system are discussed. In particular, we found that limitations must be placed on system noise, aperture, field of view and bandwidth to develop a robust wavefront coded system.

  19. Optical Imaging, Photodynamic Therapy and Optically-Triggered Combination Treatments

    PubMed Central

    Hasan, Tayyaba

    2015-01-01

    Optical imaging is becoming increasingly promising for real-time image-guided resections and combined with photodynamic therapy (PDT), a photochemistry-based treatment modality, optical approaches can be intrinsically “theranostic”. Challenges in PDT include precise light delivery, dosimetry and photosensitizer tumor localization to establish tumor selectivity, and like all other modalities, incomplete treatment and subsequent activation of molecular escape pathways are often attributable to tumor heterogeneity. Key advances in molecular imaging, target-activatable photosensitizers and optically active nanoparticles that provide both cytotoxicity and a drug release mechanism, have opened exciting avenues to meet these challenges. The focus of the review is optical imaging in the context of PDT but the general principles presented are applicable to many of the conventional approaches to cancer management. We highlight the role of optical imaging in providing structural, functional and molecular information regarding photodynamic mechanisms of action, thereby advancing PDT and PDT-based combination therapies of cancer. These advances represent a PDT renaissance with increasing applications of clinical PDT as a frontline cancer therapy working in concert with fluorescence-guided surgery, chemotherapy and radiation. PMID:26049699

  20. A flexible testbed for adaptive optics in strong turbulence

    NASA Astrophysics Data System (ADS)

    Schmidt, Jason D.; Steinbock, Michael J.; Berg, Eric C.

    2011-06-01

    In recent years, optical wave propagation through strong atmospheric turbulence and adaptive optics compensation thereof has received much attention in literature and technical meetings. At the Air Force Institute of Technology, recent simulation-based efforts in strong turbulence compensation are expanding into laboratory experiments utilizing a versatile surrogate turbulence simulator and adaptive optics system. The system can switch between using two different wavefront sensors, a Shack-Hartmann and a self-referencing interferometer. Wavefront reconstruction takes place on field programmable gate arrays, operating at kilohertz frame rates. Further, the system is able to perform reconstruction and control in software for testing of advanced algorithms (at frame rates below 10 Hz). The entire package is compact enough for transportation to other laboratories and live test facilities. This paper describes the optical layout, architecture, and initial results of real-time operation.

  1. PSF halo reduction in adaptive optics using dynamic pupil masking.

    PubMed

    Osborn, James; Myers, Richard M; Love, Gordon D

    2009-09-28

    We describe a method to reduce residual speckles in an adaptive optics system which add to the halo of the point spread function (PSF). The halo is particularly problematic in astronomical applications involving the detection of faint companions. Areas of the pupil are selected where the residual wavefront aberrations are large and these are masked using a spatial light modulator. The method is also suitable for smaller telescopes without adaptive optics as a relatively simple method to increase the resolution of the telescope. We describe the principle of the technique and show simulation results. PMID:19907514

  2. PSF halo reduction in adaptive optics using dynamic pupil masking.

    PubMed

    Osborn, James; Myers, Richard M; Love, Gordon D

    2009-09-28

    We describe a method to reduce residual speckles in an adaptive optics system which add to the halo of the point spread function (PSF). The halo is particularly problematic in astronomical applications involving the detection of faint companions. Areas of the pupil are selected where the residual wavefront aberrations are large and these are masked using a spatial light modulator. The method is also suitable for smaller telescopes without adaptive optics as a relatively simple method to increase the resolution of the telescope. We describe the principle of the technique and show simulation results.

  3. Robust control of a bimorph mirror for adaptive optics systems.

    PubMed

    Baudouin, Lucie; Prieur, Christophe; Guignard, Fabien; Arzelier, Denis

    2008-07-10

    We apply robust control techniques to an adaptive optics system including a dynamic model of the deformable mirror. The dynamic model of the mirror is a modification of the usual plate equation. We propose also a state-space approach to model the turbulent phase. A continuous time control of our model is suggested, taking into account the frequential behavior of the turbulent phase. An H(infinity) controller is designed in an infinite-dimensional setting. Because of the multivariable nature of the control problem involved in adaptive optics systems, a significant improvement is obtained with respect to traditional single input-single output methods.

  4. Optical properties of retinal tissue and the potential of adaptive optics to visualize retinal ganglion cells in vivo.

    PubMed

    Prasse, Martina; Rauscher, Franziska Georgia; Wiedemann, Peter; Reichenbach, Andreas; Francke, Mike

    2013-08-01

    Many efforts have been made to improve the diagnostic tools used to identify and to estimate the progress of ganglion cell and nerve fibre degeneration in glaucoma. Imaging by optical coherence tomography and measurements of the dimensions of the optic nerve head and the nerve fibre layer in central retinal areas is currently used to estimate the grade of pathological changes. The visualization and quantification of ganglion cells and nerve fibres directly in patients would dramatically improve glaucoma diagnostics. We have investigated the optical properties of cellular structures of retinal tissue in order to establish a means of visualizing and quantifying ganglion cells in the living retina without staining. We have characterized the optical properties of retinal tissue in several species including humans. Nerve fibres, blood vessels, ganglion cells and their cell processes have been visualized at high image resolution by means of the reflection mode of a confocal laser scanning microscope. The potential of adaptive optics in current imaging systems and the possibilities of imaging single ganglion cells non-invasively in patients are discussed.

  5. Optical imaging of fast, dynamic neurophysiological function.

    SciTech Connect

    Rector, D. M.; Carter, K. M.; Yao, X.; George, J. S.

    2002-01-01

    Fast evoked responses were imaged from rat dorsal medulla and whisker barrel cortex. To investigate the biophysical mechanisms involved, fast optical responses associated with isolated crustacean nerve stimulation were recorded using birefringence and scattered light. Such studies allow optimization of non-invasive imaging techniques being developed for use in humans.

  6. Optical encryption with selective computational ghost imaging

    NASA Astrophysics Data System (ADS)

    Zafari, Mohammad; kheradmand, Reza; Ahmadi-Kandjani, Sohrab

    2014-10-01

    Selective computational ghost imaging (SCGI) is a technique which enables the reconstruction of an N-pixel image from N measurements or less. In this paper we propose an optical encryption method based on SCGI and experimentally demonstrate that this method has much higher security under eavesdropping and unauthorized accesses compared with previous reported methods.

  7. Adaptation of commercial microscopes for advanced imaging applications

    NASA Astrophysics Data System (ADS)

    Brideau, Craig; Poon, Kelvin; Stys, Peter

    2015-03-01

    Today's commercially available microscopes offer a wide array of options to accommodate common imaging experiments. Occasionally, an experimental goal will require an unusual light source, filter, or even irregular sample that is not compatible with existing equipment. In these situations the ability to modify an existing microscopy platform with custom accessories can greatly extend its utility and allow for experiments not possible with stock equipment. Light source conditioning/manipulation such as polarization, beam diameter or even custom source filtering can easily be added with bulk components. Custom and after-market detectors can be added to external ports using optical construction hardware and adapters. This paper will present various examples of modifications carried out on commercial microscopes to address both atypical imaging modalities and research needs. Violet and near-ultraviolet source adaptation, custom detection filtering, and laser beam conditioning and control modifications will be demonstrated. The availability of basic `building block' parts will be discussed with respect to user safety, construction strategies, and ease of use.

  8. Impacts of optical turbulence on underwater imaging

    NASA Astrophysics Data System (ADS)

    Hou, Weilin; Woods, S.; Goode, W.; Jarosz, E.; Weidemann, A.

    2011-06-01

    Optical signal transmission underwater is of vital interests to both civilian and military applications. The range and signal to noise during the transmission, as a function of system and water optical properties determines the effectiveness of EO technology. These applications include diver visibility, search and rescue, mine detection and identification, and optical communications. The impact of optical turbulence on underwater imaging has been postulated and observed by many researchers. However, no quantative studies have been done until recently, in terms of both the environmental conditions, and impacts on image quality as a function of range and spatial frequencies. Image data collected from field measurements during SOTEX (Skaneateles Optical Turbulence Exercise, July 22-31, 2010) using the Image Measurement Assembly for Subsurface Turbulence (IMAST) are presented. Optical properties of the water column in the field were measured using WETLab's ac-9 and Laser In Situ Scattering and Transmissometer (LISST, Sequoia Scientific), in coordination with physical properties including CTD (Seabird), dissipation rate of kinetic energy and heat, using both the Vector velocimeter and CT combo (Nortek and PME), and shear probe based Vertical Microstructure Profiler (VMP, Rockland). The strong stratification structure in the water column provides great opportunity to observe various dissipation strengths throughout the water column, which corresponds directly with image quality as shown. Initial results demonstrate general agreement between data collected and model prediction, while discrepancies between measurements and model suggest higher spatial and temporal observations are needed in the future.

  9. Optical Data Compression in Time Stretch Imaging

    PubMed Central

    Chen, Claire Lifan; Mahjoubfar, Ata; Jalali, Bahram

    2015-01-01

    Time stretch imaging offers real-time image acquisition at millions of frames per second and subnanosecond shutter speed, and has enabled detection of rare cancer cells in blood with record throughput and specificity. An unintended consequence of high throughput image acquisition is the massive amount of digital data generated by the instrument. Here we report the first experimental demonstration of real-time optical image compression applied to time stretch imaging. By exploiting the sparsity of the image, we reduce the number of samples and the amount of data generated by the time stretch camera in our proof-of-concept experiments by about three times. Optical data compression addresses the big data predicament in such systems. PMID:25906244

  10. NAOMI: nanoparticle assisted optical molecular imaging

    NASA Astrophysics Data System (ADS)

    Faber, Dirk J.; van Velthoven, Mirjam E. J.; de Bruin, Martijn; Aalders, Maurice C. G.; Verbraak, Frank D.; Graf, Christina; van Leeuwen, Ton G.

    2006-02-01

    Our first steps towards nanoparticle assisted, optical molecular imaging (NAOMI) using OCT as the imaging modality are presented. We derive an expression to estimate the sensitivity of this technique. We propose to use nanoparticles based on biodegradable polymers, loaded with suitable dyes as contrast agent, and outline a method for establishing their desired optical properties prior to synthesis. This report presents preliminary results of our investigation on the use of nanoshells to serve as contrast agents We injected nanoshells with specific contrast features in the 800 nm wavelength region in excised porcine eyes. The nanoshells showed up as bright reflecting structures in the OCT images, which confirm their potential as contrast agents.

  11. Positron emission tomography and optical tissue imaging

    DOEpatents

    Falen, Steven W.; Hoefer, Richard A.; Majewski, Stanislaw; McKisson, John; Kross, Brian; Proffitt, James; Stolin, Alexander; Weisenberger, Andrew G.

    2012-05-22

    A mobile compact imaging system that combines both PET imaging and optical imaging into a single system which can be located in the operating room (OR) and provides faster feedback to determine if a tumor has been fully resected and if there are adequate surgical margins. While final confirmation is obtained from the pathology lab, such a device can reduce the total time necessary for the procedure and the number of iterations required to achieve satisfactory resection of a tumor with good margins.

  12. Enhanced neutron imaging detector using optical processing

    SciTech Connect

    Hutchinson, D.P.; McElhaney, S.A.

    1992-08-01

    Existing neutron imaging detectors have limited count rates due to inherent property and electronic limitations. The popular multiwire proportional counter is qualified by gas recombination to a count rate of less than 10{sup 5} n/s over the entire array and the neutron Anger camera, even though improved with new fiber optic encoding methods, can only achieve 10{sup 6} cps over a limited array. We present a preliminary design for a new type of neutron imaging detector with a resolution of 2--5 mm and a count rate capability of 10{sup 6} cps pixel element. We propose to combine optical and electronic processing to economically increase the throughput of advanced detector systems while simplifying computing requirements. By placing a scintillator screen ahead of an optical image processor followed by a detector array, a high throughput imaging detector may be constructed.

  13. The optical design of a visible adaptive optics system for the Magellan Telescope

    NASA Astrophysics Data System (ADS)

    Kopon, Derek

    The Magellan Adaptive Optics system will achieve first light in November of 2012. This AO system contains several subsystems including the 585-actuator concave adaptive secondary mirror, the Calibration Return Optic (CRO) alignment and calibration system, the CLIO 1-5 microm IR science camera, the movable guider camera and active optics assembly, and the W-Unit, which contains both the Pyramid Wavefront Sensor (PWFS) and the VisAO visible science camera. In this dissertation, we present details of the design, fabrication, assembly, alignment, and laboratory performance of the VisAO camera and its optical components. Many of these components required a custom design, such as the Spectral Differential Imaging Wollaston prisms and filters and the coronagraphic spots. One component, the Atmospheric Dispersion Corrector (ADC), required a unique triplet design that had until now never been fabricated and tested on sky. We present the design, laboratory, and on-sky results for our triplet ADC. We also present details of the CRO test setup and alignment. Because Magellan is a Gregorian telescope, the ASM is a concave ellipsoidal mirror. By simulating a star with a white light point source at the far conjugate, we can create a double-pass test of the whole system without the need for a real on-sky star. This allows us to test the AO system closed loop in the Arcetri test tower at its nominal design focal length and optical conjugates. The CRO test will also allow us to calibrate and verify the system off-sky at the Magellan telescope during commissioning and periodically thereafter. We present a design for a possible future upgrade path for a new visible Integral Field Spectrograph. By integrating a fiber array bundle at the VisAO focal plane, we can send light to a pre-existing facility spectrograph, such as LDSS3, which will allow 20 mas spatial sampling and R˜1,800 spectra over the band 0.6-1.05 microm. This would be the highest spatial resolution IFU to date, either

  14. Use of electrochromic materials in adaptive optics

    NASA Astrophysics Data System (ADS)

    Kammler, Daniel R.; Yelton, William G.; Sweatt, William C.; Verley, Jason C.

    2005-08-01

    Electrochromic (EC) materials are used in "smart" windows that can be darkened by applying a voltage across an EC stack on the window. The associated change in refractive index (n) in the EC materials might allow their use in tunable or temperature-insensitive Fabry-Perot filters and transmissive-spatial-light-modulators (SLMs). The authors are conducting a preliminary evaluation of these materials in many applications, including target-in-the-loop systems. Data on tungsten oxide, WO3, the workhorse EC material, indicate that it's possible to achieve modest changes in n with only slight increases in absorption between the visible and ~10 μm. This might enable construction of a tunable Fabry-Perot filter consisting of an active EC layer (e.g. WO3) and a proton conductor (e.g.Ta2O5) sandwiched between two gold electrodes. A SLM might be produced by replacing the gold with a transparent conductor (e.g.