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Sample records for adaptive optics real-time

  1. Adaptive optics real time processing design for the advanced technology solar telescope

    NASA Astrophysics Data System (ADS)

    Richards, Kit

    2012-07-01

    The four meter Advanced Technology Solar Telescope (ATST) adaptive optics (AO) system will require at least twenty-four times the real time processing power as the Dunn Solar Telescope AO system. An FPGA solution for ATST AO real time processing is being pursued instead of the parallel DSP approach used for the Dunn AO76 system. An analysis shows FPGAs will have lower latency and lower hardware cost than an equivalent DSP solution. Interfacing to the proposed high speed camera and the deformable mirror will be simpler and have lower latency than with DSPs. This paper will discuss the current design and progress toward implementing the FPGA solution.

  2. A compact multi-core CPU based adaptive optics real-time controller

    NASA Astrophysics Data System (ADS)

    Chen, Shanqiu; Zhao, Enyi; Xu, Bing; Ye, Yutang

    2014-09-01

    The performance of Adaptive Optics (AO) real-time controller based on Central Processing Unit (CPU) has significantly progressed due to the introduction of the high speed frame-grabber and a 4-cores CPU, which make it possible to process at frequency over 2000 Hz for 4-meter-class telescope and to integrate the real-time task and the user interface program in this compact device. The detailed architecture of this computation system is demonstrated in this paper, and the performance and suitability of this architecture is also discussed by measuring the latency of the controller processing via an adaptive optics emulator system with 16 times 16 and 32 times 32 sub-aperture, and the overall typical processing time is 61 us and 322 us respectively. Test result turns out that it is well suited for the next generation 4-meter-class adaptive optics system and it is possible to process at frequency over 2000 Hz for a 3000-element AO system in 10- meter-class telescope with one board of art-of-the-state computer and a frame-grabber. Comparison with GPU and FPGA based architecture is also discussed in this paper.

  3. DRAGON, the Durham real-time, tomographic adaptive optics test bench: progress and results

    NASA Astrophysics Data System (ADS)

    Reeves, Andrew P.; Myers, Richard M.; Morris, Timothy J.; Basden, Alastair G.; Bharmal, Nazim A.; Rolt, Stephen; Bramall, David G.; Dipper, Nigel A.; Younger, Edward J.

    2014-08-01

    DRAGON is a real-time, tomographic Adaptive Optics test bench currently under development at Durham University. Optical and mechanical design work for DRAGON is now complete, and the system is close to becoming fully operational. DRAGON emulates current 4.2 m and 8 m telescopes, and can also be used to investigate ELT scale issues. The full system features 4 Laser Guide Star (LGS) Wavefront Sensors (WFS), 3 Natural Guide Star (NGS) WFSs and one Truth Sensor, all of which are 31 × 31 sub-aperture Shack-Hartmann WFS. Two Deformable Mirrors (DMs), a Boston MEMS Kilo DM and a Xinetics 97 actuator DM, correct for turbulence induced aberrations and these can be configured to be either open or closed loop of the WFS. A novel method of LGS emulation is implemented which includes the effects of uplink turbulence and elongation in real-time. The atmosphere is emulated by 4 rotating phase screens which can be translated in real-time to replicate altitude evolution of turbulent layers. DRAGON will be used to extensively study tomographic AO algorithms, such as those required for Multi-Object AO. As DRAGON has been designed to be compatible with CANARY, the MOAO demonstrator, results can be compared to those from the CANARY MOAO demonstrator on the 4.2m William Herschel Telescope. We present here an overview of the current status of DRAGON and some early results, including investigations into the validity of the LGS emulation method.

  4. Development of a scalable generic platform for adaptive optics real time control

    NASA Astrophysics Data System (ADS)

    Surendran, Avinash; Burse, Mahesh P.; Ramaprakash, A. N.; Parihar, Padmakar

    2015-06-01

    The main objective of the present project is to explore the viability of an adaptive optics control system based exclusively on Field Programmable Gate Arrays (FPGAs), making strong use of their parallel processing capability. In an Adaptive Optics (AO) system, the generation of the Deformable Mirror (DM) control voltages from the Wavefront Sensor (WFS) measurements is usually through the multiplication of the wavefront slopes with a predetermined reconstructor matrix. The ability to access several hundred hard multipliers and memories concurrently in an FPGA allows performance far beyond that of a modern CPU or GPU for tasks with a well-defined structure such as Adaptive Optics control. The target of the current project is to generate a signal for a real time wavefront correction, from the signals coming from a Wavefront Sensor, wherein the system would be flexible to accommodate all the current Wavefront Sensing techniques and also the different methods which are used for wavefront compensation. The system should also accommodate for different data transmission protocols (like Ethernet, USB, IEEE 1394 etc.) for transmitting data to and from the FPGA device, thus providing a more flexible platform for Adaptive Optics control. Preliminary simulation results for the formulation of the platform, and a design of a fully scalable slope computer is presented.

  5. SPARTA: the ESO standard platform for adaptive optics real time applications

    NASA Astrophysics Data System (ADS)

    Fedrigo, Enrico; Donaldson, Robert; Soenke, Christian; Myers, Richard; Goodsell, Stephen; Geng, Deli; Saunter, Chris; Dipper, Nigel

    2006-06-01

    ESO is starting a number of new projects collectively called Second Generation VLT instrumentation. Several of them will use Adaptive Optics (AO). In comparison with today's ESO AO systems, the 2nd Generation VLT AO systems will be much bigger (in terms of degrees of freedom) and faster (in terms of loop frequency). Consequently the Real-Time Computer controlling these AO systems will be significantly bigger and more challenging to build compared with today's AO systems in operation. To support the new requirements ESO started the development of a common flexible platform called SPARTA for Standard Platform for Adaptive optics Real Time Applications. The guidelines along which SPARTA is developed recognize the importance of industry standards over custom development to lower the development costs, ease the maintenance and make the system upgradeable thus delivering the performance required. SPARTA is based on a hybrid architecture that comprises all the major computing architectures available today: the high computational throughput is achieved through the combination of FPGA and DSP usage, where DSP are used as fast coprocessors and FPGA are used as front and as communication infrastructure, thus guaranteeing also the low latency. The flexibility is spread between the usage of both high-end CPUs and again the DSPs. All three technologies are organized in a parallel system interconnected by fast serial fabrics based on standard protocols. External input / output interfaces are also based on industry standard protocols, thus enabling the usage of commercially available tools for development and testing.

  6. Real-Time Wavefront Control for the PALM-3000 High Order Adaptive Optics System

    NASA Technical Reports Server (NTRS)

    Truong, Tuan N.; Bouchez, Antonin H.; Dekany, Richard G.; Guiwits, Stephen R.; Roberts, Jennifer E.; Troy, Mitchell

    2008-01-01

    We present a cost-effective scalable real-time wavefront control architecture based on off-the-shelf graphics processing units hosted in an ultra-low latency, high-bandwidth interconnect PC cluster environment composed of modules written in the component-oriented language of nesC. The architecture enables full-matrix reconstruction of the wavefront at up to 2 KHz with latency under 250 us for the PALM-3000 adaptive optics systems, a state-of-the-art upgrade on the 5.1 meter Hale Telescope that consists of a 64 x 64 subaperture Shack-Hartmann wavefront sensor and a 3368 active actuator high order deformable mirror in series with a 241 active actuator tweeter DM. The architecture can easily scale up to support much larger AO systems at higher rates and lower latency.

  7. Adaptive Optics Real-time Control Systems for the E-ELT

    NASA Astrophysics Data System (ADS)

    Dipper, Nigel; Basden, Alastair; Bitenc, Urban; Myers, Richard; Richards, Andrew; Younger, Eddy

    2013-12-01

    The next generation of large telescopes will depend critically on Adaptive Optics. The instrumentation now proposed for ELTs makes substantial demands on computing power for real-time control. These demands will be met by a combination of novel algorithms and the use of new developments in the world of high power computing. This poster will summarise the developments made in meeting this challenge at the CfAI in Durham and our research and development plan over the next few years. We will demonstrate what can be done on an ELT scale with existing hardware (FPGA, GPU and CPU) and to what aspects of the real-time control system these technologies are best applied. In addition, we will report on initial attempts at Durham to abstract the hardware from the software, using high-level languages such as OpenCL. This will be critical to making software for the E-ELT 'future proof' allowing the easy introduction of new computing technology that will emerge over the long development period of ELT instrumentation.

  8. Thirty Meter Telescope narrow-field infrared adaptive optics system real-time controller prototyping results

    NASA Astrophysics Data System (ADS)

    Smith, Malcolm; Kerley, Dan; Chapin, Edward L.; Dunn, Jennifer; Herriot, Glen; Véran, Jean-Pierre; Boyer, Corinne; Ellerbroek, Brent; Gilles, Luc; Wang, Lianqi

    2016-07-01

    Prototyping and benchmarking was performed for the Real-Time Controller (RTC) of the Narrow Field InfraRed Adaptive Optics System (NFIRAOS). To perform wavefront correction, NFIRAOS utilizes two deformable mirrors (DM) and one tip/tilt stage (TTS). The RTC receives wavefront information from six Laser Guide Star (LGS) Shack- Hartmann WaveFront Sensors (WFS), one high-order Natural Guide Star Pyramid WaveFront Sensor (PWFS) and multiple low-order instrument detectors. The RTC uses this information to determine the commands to send to the wavefront correctors. NFIRAOS is the first light AO system for the Thirty Meter Telescope (TMT). The prototyping was performed using dual-socket high performance Linux servers with the real-time (PREEMPT_RT) patch and demonstrated the viability of a commercial off-the-shelf (COTS) hardware approach to large scale AO reconstruction. In particular, a large custom matrix vector multiplication (MVM) was benchmarked which met the required latency requirements. In addition all major inter-machine communication was verified to be adequate using 10Gb and 40Gb Ethernet. The results of this prototyping has enabled a CPU-based NFIRAOS RTC design to proceed with confidence and that COTS hardware can be used to meet the demanding performance requirements.

  9. Development status on the real-time controller for solar multi-conjugate adaptive optics system

    NASA Astrophysics Data System (ADS)

    Kong, Lin; Zhu, Lei; Rao, Changhui

    2016-10-01

    Solar observations are performed over an extended field of view (FoV) and the isoplanatic patch which conventional adaptive optics (AO) provides diffraction limited resolution is a severe limitation. Multi-conjugate Adaptive Optics (MCAO) can be used to extend the corrected FoV of AO system. Compared to AO, MCAO which usually utilizes a wide-field Shack-Hartmann wavefront sensor to control multiple deformable mirrors(DMs) is more complicated. Because the Sun is an extended object, correlation algorithms are applied to detect gradients in solar MCAO system. Moreover, due to the fast evolving daytime seeing conditions and the fact that much science has to be done at visible wavelengths, a very high closed-loop bandwidth is also required. The computation and delay development of the real-time controller (RTC) in solar MCAO system is more challenging than that in night-time MCAO system. This paper reviews the solar MCAO techniques and systems in the world, especially emphasizes the framework and implementation of the RTC. The development of MCAO in China is also introduced. An outlook of the RTC for the solar MCAO system in development is given.

  10. Adaptive Optics Correction in Real-Time for Dynamic Wavefront Errors

    DTIC Science & Technology

    1990-03-15

    This paper reports on the principles for the use of, and the experimental results obtained from, an adaptive optics system for correcting dynamic...control system. Keywords: Adaptive optics ; Wavefront sensing; Deformable mirror; Chinese translations.

  11. DARTS: a low-cost high-performance FPGA implemented real-time control platform for adaptive optics

    NASA Astrophysics Data System (ADS)

    Goodsell, S. J.; Dipper, N. A.; Geng, D.; Myers, R. M.; Saunter, C. D.

    2005-08-01

    Durham University's Centre for Advanced Instrumentation (CfAI) are currently producing a generic high-performance low-cost real-time control system (RTCS) for adaptive optics (AO) based on Field Programmable Gate Array (FPGA) technology. This platform, labelled DARTS, 'Durham Adaptive optics Real Time System', will primarily be used as the controller for Durham's enhanced Rayleigh Technical Demonstrator (RTD) system. However, DARTS could be used as a low latency control system for existing AO instruments or could be used for future 'budget' AO Natural Guide Star (NGS) and/or Laser Guide Star (LGS) RTCS. DARTS uses an FPGA device to host an end-to-end modular real-time AO pipeline connected to a Wishbone control bus. The FPGA takes advantage of the pipeline's highly parallel computationally intensive tasks which usually are calculated in series by a system processor. DARTS hopes to increase the obtainable control loop frequency and reduce the computational latency of the RTD's RTCS. DARTS is capable of high bandwidth I/O due to the implementation of the serial Front Panel Data Port (sFPDP) industrial protocol. The hardware's I/O design is modular, allowing for the future connection of various WFSs and DMs via signal converters. Various communications architectures are suggested to allow non real-time configuration and visualisation data to flow between the wishbone control bus and a processing device, either externally or internally to the FPGA device. This paper reveals the current status of the project.

  12. Adaptive optics at the University of Hawaii II: control system with real-time diagnostics

    NASA Astrophysics Data System (ADS)

    Anuskiewicz, Jim; Northcott, Malcolm J.; Graves, J. Elon

    1994-05-01

    The University of Hawaii experimental adaptive optics system is controlled by dual SPARC single board computers on a VME backplane. One processor is dedicated to the feedback loop. The second processor manages loop data flow to a workstation and transfers new control parameters to the loop processor without stopping the loop. This system facilitates cause-effect analysis of the various system parameters.

  13. Real-time wavefront reconstruction for a 512-subaperture adaptive optical system

    NASA Astrophysics Data System (ADS)

    Johnson, Paul A.; Trissel, Richard G.; Cuellar, E. L.; Arnold, Robert L.; Sandler, David G.

    1992-01-01

    This paper describes a system capable of real-time wavefront reconstruction for a 512 subaperture shearing interferometer. The system was designed to interface with a 1536 channel (512 segment) deformable mirror for atmospheric compensation using an artificial beacon. The phase gradients were measured using a shearing interferometer operating at two distinct shear lengths with quantum limited performance at 200 photons per subaperture. A 128 node parallel processor performed a sparse matrix multiply to reconstruct the phasefront in real time. The matrix truncation technique used allowed 90% of the elements to be removed with only minor penalty in wavefront accuracy.

  14. Real-time processor based on GPU for on-line performance evaluation of the 127-element adaptive optics system

    NASA Astrophysics Data System (ADS)

    Zhou, Han; Zhou, Lu-chun

    2013-08-01

    In this paper, a real-time on-line performance evaluation processor based on graphic processing unit (GPU) for adaptive optics (AO) system is presented, aiming to monitor the 127-element AO system during its close-loop work by quantifying its correction results, which can provide reference to improve the performance of the system. Since there is a contradiction between the heavy computation burden and the real-time processing requirement, we modified operations and algorithms to fit the CPU-GPU heterogeneous environment, in which GPU is used to handle the complex computation but simple logicality, and CPU is assigned to undertake data transportation between internal storage and video memory,as well as some small-scale computations. In the real-time processor, performance parameters to be computed include peak-valley (PV) and root-mean-square (RMS) of near-field wavefront phase, point spread function (PSF), full width half maximum (FWHM) of far-field image,modulation transfer function (MTF) and Strehl ratio (SR). And the inputs are residual slopes obtained from Hartmann wavefront sensor of 127-element AO system. By computation 4096 frames of parameters, the average rate by single precision is 4.11ms/frame.

  15. Thirty Meter Telescope (TMT) Narrow Field Infrared Adaptive Optics System (NFIRAOS) real-time controller preliminary architecture

    NASA Astrophysics Data System (ADS)

    Kerley, Dan; Smith, Malcolm; Dunn, Jennifer; Herriot, Glen; Véran, Jean-Pierre; Boyer, Corinne; Ellerbroek, Brent; Gilles, Luc; Wang, Lianqi

    2016-08-01

    The Narrow Field Infrared Adaptive Optics System (NFIRAOS) is the first light Adaptive Optics (AO) system for the Thirty Meter Telescope (TMT). A critical component of NFIRAOS is the Real-Time Controller (RTC) subsystem which provides real-time wavefront correction by processing wavefront information to compute Deformable Mirror (DM) and Tip/Tilt Stage (TTS) commands. The National Research Council of Canada - Herzberg (NRC-H), in conjunction with TMT, has developed a preliminary design for the NFIRAOS RTC. The preliminary architecture for the RTC is comprised of several Linux-based servers. These servers are assigned various roles including: the High-Order Processing (HOP) servers, the Wavefront Corrector Controller (WCC) server, the Telemetry Engineering Display (TED) server, the Persistent Telemetry Storage (PTS) server, and additional testing and spare servers. There are up to six HOP servers that accept high-order wavefront pixels, and perform parallelized pixel processing and wavefront reconstruction to produce wavefront corrector error vectors. The WCC server performs low-order mode processing, and synchronizes and aggregates the high-order wavefront corrector error vectors from the HOP servers to generate wavefront corrector commands. The Telemetry Engineering Display (TED) server is the RTC interface to TMT and other subsystems. The TED server receives all external commands and dispatches them to the rest of the RTC servers and is responsible for aggregating several offloading and telemetry values that are reported to other subsystems within NFIRAOS and TMT. The TED server also provides the engineering GUIs and real-time displays. The Persistent Telemetry Storage (PTS) server contains fault tolerant data storage that receives and stores telemetry data, including data for Point-Spread Function Reconstruction (PSFR).

  16. Real-time optical tweezing

    NASA Astrophysics Data System (ADS)

    Rahman, Shah Mohammed Tamzidur

    In this thesis a new approach called ‘space-time-wavelength mapping’ has been developed for real-time electronic control of optical tweezers. The proposed technique enables precise control of optical signals in space, time, and frequency through time-domain dispersion and diffractive optics, which in turn enables generation of controlled radiation forces acting on small particles. In this study we show that 150 fs ultrafast optical pulses can be dispersed in time and space to achieve a 20 μm x 2 μm focused elliptical beam. The force field at the focal plane of the beam is dependent on local intensity gradients along the plane. The spatial intensity profile can be electronically controlled by assigning local power levels to each wavelength using time-domain RF modulation of dispersed pulses, and sending each wavelength, and hence the assigned power level, to a specific location in space through diffractive optics. We show that by choosing the appropriate RF waveform, one is able to create force fields for cell stretching and compression as well as multiple force hot-spots (of >200 pN force per pulse) for attractive and repulsive forces. A detailed theoretical model and simulation results from a proposed experimental setup are presented. This approach is significantly more advantageous in terms of flexibility and control, compared to conventional optical tweezers that require mechanical steering or holographic optical tweezers that produce undesired ‘ghost traps’. In addition, it is shown how the technique can also be extended to create tunable 2D force field distributions using a virtually-imaged phased-array (VIPA).

  17. Fixed-point vs. floating-point arithmetic comparison for adaptive optics real-time control computation

    NASA Astrophysics Data System (ADS)

    Martín-Hernando, Yolanda; Rodríguez-Ramos, Luis F.; Garcia-Talavera, Marcos R.

    2008-07-01

    Most computers in the past have been equipped with floating point processing capabilities, allowing an easy and brute-force solution for the machine computation errors, not requiring any specific tailoring of the computation in nearly hundred percent of situations. However, the computation needed for the adaptive optics real-time control in 30-50 meter telescopes is big enough to cause trouble to conventional von-Neumann processors, even if Moore's Law is valid for the next years. Field Programmable Gate Array (FPGAs) have been proposed as a viable alternative to cope with such computation needs[1,2], but--at least today's chips--will require fixed-point arithmetic to be used instead. It is then important to evaluate up to what point the accuracy and stability of the control system will be affected by this limitation. This paper presents the simulation and laboratory results of the comparison between both arithmetics, specifically evaluated in an adaptive optics system. The real-time controller has been modeled as black box having as input the wavefront sensor camera digital output data, providing a digital output to the actuators of the deformable mirror, and with the task of internally computing all outputs from the inputs. MATLAB fixed-point library has been used to evaluate the effect of different precision lengths (5-10 fractional bits) in the computation of the Shack-Hartmann subaperture centroid, in comparison with the reference 64-bit floating-point arithmetic and with the noise floor of the real system, concluding that the effect of the limited precision can be overcome by adequately selecting the number of fractional bits used in the representation, and tailoring that number with the needs at every step of the algorithm.

  18. Real-time optical flow computation based on adaptive color quantization by competitive neural networks

    NASA Astrophysics Data System (ADS)

    Grana Romay, Manuel; Echave, Imanol

    1999-08-01

    In this paper we prose the application of the codebook computed by the Self Organizing Map as a smoothing filter, the QV Bayesian Filter, for the preprocessing of the image sequences. The optical flow is then robustly and efficiently computed over the filtered imags applying a correlation approach at the pixel level.

  19. Real-Time Nonlinear Optical Information Processing.

    DTIC Science & Technology

    1979-06-01

    operations aree presented. One approach realizes the halftone method of nonlinear optical processing in real time by replacing the conventional...photographic recording medium with a real-time image transducer. In the second approach halftoning is eliminated and the real-time device is used directly

  20. The real-time control system for the CANARY multi-object adaptive optics on-sky demonstrator

    NASA Astrophysics Data System (ADS)

    Dipper, N. A.; Basden, A.; Looker, N. E.; Gendron, E.; Geng, D.; Gratadour, D.; Hubert, Z.; Vidal, F.; Myers, R. M.; Rousset, G.; Sevin, A.; Younger, E. J.

    2010-07-01

    CANARY is a Multi-Object Adaptive Optics (MOAO) system designed to demonstrate the AO aspects of proposed EELT instruments such as the multi-object spectrograph EAGLE. The first phase of Canary will be executed on the 4.2m William Herschel Telescope in 2010. We describe here the AO Real-time Control System (RTCS) for Canary. This is based on a distributed architecture of components interconnected by a fast serial fabric (sFPDP). The hardware used is a hybrid of FPGA and CPU technology. The middleware used for system data telemetry and control is based on CORBA and the publish/subscribe pattern. The system is designed to be easily modified and extended for the later, higher order, phases of CANARY. In order to provide the increase in computational power required in higher order systems, the current CPU technology can be readily replaced by acceleration hardware based on FPGA or GPU technologies. The Canary RTCS thus provides a test-bed for these new technologies that will be required for E-ELT instruments. These design concepts can be developed to provide an RTCS for E-ELT instruments and are in line with those under consideration by ESO for the E-ELT AO systems to which instruments such as EAGLE will be required to interface.

  1. Real time optical figure sensor

    NASA Technical Reports Server (NTRS)

    Robertson, H. J.

    1973-01-01

    Mirrors produced for various optical systems require precise surface finishing. Sensor, developed for measuring mirror surface, is compensated for interferences from temperature and air disturbances and is capable of measuring mirrors with diameters of up to 2 meters.

  2. Real-time adaptive optics testbed to investigate point-ahead angle in pre-compensation of Earth-to-GEO optical communication.

    PubMed

    Leonhard, Nina; Berlich, René; Minardi, Stefano; Barth, Alexander; Mauch, Steffen; Mocci, Jacopo; Goy, Matthias; Appelfelder, Michael; Beckert, Erik; Reinlein, Claudia

    2016-06-13

    We explore adaptive optics (AO) pre-compensation for optical communication between Earth and geostationary (GEO) satellites in a laboratory experiment. Thus, we built a rapid control prototyping breadboard with an adjustable point-ahead angle where downlink and uplink can operate both at 1064 nm and 1550 nm wavelength. With our real-time system, beam wander resulting from artificial turbulence was reduced such that the beam hits the satellite at least 66% of the time as compared to merely 3% without correction. A seven-fold increase of the average Strehl ratio to (28 ± 15)% at 18 μrad point-ahead angle leads to a considerable reduction of the calculated fading probability. These results make AO pre-compensation a viable technique to enhance Earth-to-GEO optical communication.

  3. Adaptive 4~64 QAM real-time coherent optical transmission over 320 km with FPGA-based transmitter and receiver.

    PubMed

    Yoshida, Masato; Hirooka, Toshihiko; Kasai, Keisuke; Nakazawa, Masataka

    2014-06-30

    We demonstrate the first real-time adaptive optical coherent QAM transmission with variable multiplicities (4-, 16- and 64-QAM) using an FPGA-based transmitter and receiver. Rate-variable transmission (20~60 Gbit/s) was successfully achieved with a polarization multiplexing scheme at 5 Gsymbol/s over 320 km, where the OSNR margins were increased by 9 and 17 dB, respectively, by changing the modulation level from 64 to 16 and 4.

  4. EPICS and VxWorks for real-time motion control of the Altair adaptive optics instrument for Gemini

    NASA Astrophysics Data System (ADS)

    Ebbers, Angelic W.; Dunn, Jennifer

    2002-12-01

    The Instrument Group in the National Research Council of Canada's Herzberg Institute of Astrophysics develops instrumentation for large Astronomical telescopes including Altair (Gemini's ALTitude conjugate Adaptive optics for the InfraRed). This paper discusses how we responded to a need for adaptive intelligence and complex choreography and sequencing of mechanisms in the design of Altair's motion control systems. Our primary goal has been to maximize code reusability without sacrificing performance or flexibility.

  5. Experimental investigations in transmission performance of real-time long-reach adaptively modulated direct-detection optical-orthogonal frequency division multiplexing systems

    NASA Astrophysics Data System (ADS)

    Chen, Ming; He, Jing; Tang, Jin; Chen, Lin

    2014-09-01

    A real-time base-band orthogonal frequency division multiplexing (OFDM) transceiver with symbol synchronization, channel equalization, sampling clock frequency synchronization, and adaptive modulation technique is successfully implemented by field programmable gate arrays and a 2.5-GSps digital-to-analog converter and analog-to-digital converter. The real-time optical OFDM signal at a raw bit rate of 5.156 Gbps within about 1.1-GHz bandwidth transmission over 100-km standard single-mode fiber (SSMF) is experimentally investigated in a simple intensity-modulation and direct-detection system. The experimental results show that the real-time system has a good bit error rate (BER) performance by using an adaptive modulation technique according to the conditions on the subchannels. After 100-km SSMF transmission, at a BER of 1×10-3, the power penalty is <1 dB. Moreover, there is a negligible penalty between the off-line and real-time digital signal processing results.

  6. Nonlinear Real-Time Optical Signal Processing.

    DTIC Science & Technology

    1984-10-01

    DTIC ELECTE I B IIMAGE PROCESSING INSTITUTE 84 11 26 107 UNCLASSIFIED SECURITY CLASSIFICATION OF THIS PAGE (When Dota Entered), REPORT DOCUMENTATION...30, 1984 N NONLINEAR REAL-TIME OPTICAL SIGNAL PROCESSING i E~ A.A. Sawchuk, Principal Investigator T.C. Strand and A.R. Tanguay. Jr. October 1, 1984...RDepartment of Electrical Engineering Image Processing institute University of Southern California University Park-MC 0272 Los Angeles, California

  7. Real-time optical image processing techniques

    NASA Technical Reports Server (NTRS)

    Liu, Hua-Kuang

    1988-01-01

    Nonlinear real-time optical processing on spatial pulse frequency modulation has been pursued through the analysis, design, and fabrication of pulse frequency modulated halftone screens and the modification of micro-channel spatial light modulators (MSLMs). Micro-channel spatial light modulators are modified via the Fabry-Perot method to achieve the high gamma operation required for non-linear operation. Real-time nonlinear processing was performed using the halftone screen and MSLM. The experiments showed the effectiveness of the thresholding and also showed the needs of higher SBP for image processing. The Hughes LCLV has been characterized and found to yield high gamma (about 1.7) when operated in low frequency and low bias mode. Cascading of two LCLVs should also provide enough gamma for nonlinear processing. In this case, the SBP of the LCLV is sufficient but the uniformity of the LCLV needs improvement. These include image correlation, computer generation of holograms, pseudo-color image encoding for image enhancement, and associative-retrieval in neural processing. The discovery of the only known optical method for dynamic range compression of an input image in real-time by using GaAs photorefractive crystals is reported. Finally, a new architecture for non-linear multiple sensory, neural processing has been suggested.

  8. Variational optical flow computation in real time.

    PubMed

    Bruhn, Andrés; Weickert, Joachim; Feddern, Christian; Kohlberger, Timo; Schnörr, Christoph

    2005-05-01

    This paper investigates the usefulness of bidirectional multigrid methods for variational optical flow computations. Although these numerical schemes are among the fastest methods for solving equation systems, they are rarely applied in the field of computer vision. We demonstrate how to employ those numerical methods for the treatment of variational optical flow formulations and show that the efficiency of this approach even allows for real-time performance on standard PCs. As a representative for variational optic flow methods, we consider the recently introduced combined local-global method. It can be considered as a noise-robust generalization of the Horn and Schunck technique. We present a decoupled, as well as a coupled, version of the classical Gauss-Seidel solver, and we develop several multgrid implementations based on a discretization coarse grid approximation. In contrast, with standard bidirectional multigrid algorithms, we take advantage of intergrid transfer operators that allow for nondyadic grid hierarchies. As a consequence, no restrictions concerning the image size or the number of traversed levels have to be imposed. In the experimental section, we juxtapose the developed multigrid schemes and demonstrate their superior performance when compared to unidirectional multgrid methods and nonhierachical solvers. For the well-known 316 x 252 Yosemite sequence, we succeeded in computing the complete set of dense flow fields in three quarters of a second on a 3.06-GHz Pentium4 PC. This corresponds to a frame rate of 18 flow fields per second which outperforms the widely-used Gauss-Seidel method by almost three orders of magnitude.

  9. Real-time adaptive radiometric compensation.

    PubMed

    Grundhöfer, Anselm; Bimber, Oliver

    2008-01-01

    Recent radiometric compensation techniques make it possible to project images onto colored and textured surfaces. This is realized with projector-camera systems by scanning the projection surface on a per-pixel basis. Using the captured information, a compensation image is calculated that neutralizes geometric distortions and color blending caused by the underlying surface. As a result, the brightness and the contrast of the input image is reduced compared to a conventional projection onto a white canvas. If the input image is not manipulated in its intensities, the compensation image can contain values that are outside the dynamic range of the projector. These will lead to clipping errors and to visible artifacts on the surface. In this article, we present an innovative algorithm that dynamically adjusts the content of the input images before radiometric compensation is carried out. This reduces the perceived visual artifacts while simultaneously preserving a maximum of luminance and contrast. The algorithm is implemented entirely on the GPU and is the first of its kind to run in real-time.

  10. Real Time & Power Efficient Adaptive - Robust Control

    NASA Astrophysics Data System (ADS)

    Ioan Gliga, Lavinius; Constantin Mihai, Cosmin; Lupu, Ciprian; Popescu, Dumitru

    2017-01-01

    A design procedure for a control system suited for dynamic variable processes is presented in this paper. The proposed adaptive - robust control strategy considers both adaptive control advantages and robust control benefits. It estimates the degradation of the system’s performances due to the dynamic variation in the process and it then utilizes it to determine when the system must be adapted with a redesign of the robust controller. A single integral criterion is used for the identification of the process, and for the design of the control algorithm, which is expressed in direct form, through a cost function defined in the space of the parameters of both the process and the controller. For the minimization of this nonlinear function, an adequate mathematical programming minimization method is used. The theoretical approach presented in this paper was validated for a closed loop control system, simulated in an application developed in C. Because of the reduced number of operations, this method is suitable for implementation on fast processes. Due to its effectiveness, it increases the idle time of the CPU, thereby saving electrical energy.

  11. Nonlinear Real-Time Optical Signal Processing.

    DTIC Science & Technology

    1981-06-30

    bandwidth and space-bandwidth products. Real-time homonorphic and loga- rithmic filtering by halftone nonlinear processing has been achieved. A...Page ABSTRACT 1 1. RESEARCH OBJECTIVES AND PROGRESS 3 I-- 1.1 Introduction and Project overview 3 1.2 Halftone Processing 9 1.3 Direct Nonlinear...time homomorphic and logarithmic filtering by halftone nonlinear processing has been achieved. A detailed analysis of degradation due to the finite gamma

  12. Real-time turbulence profiling with a pair of laser guide star Shack-Hartmann wavefront sensors for wide-field adaptive optics systems on large to extremely large telescopes.

    PubMed

    Gilles, L; Ellerbroek, B L

    2010-11-01

    Real-time turbulence profiling is necessary to tune tomographic wavefront reconstruction algorithms for wide-field adaptive optics (AO) systems on large to extremely large telescopes, and to perform a variety of image post-processing tasks involving point-spread function reconstruction. This paper describes a computationally efficient and accurate numerical technique inspired by the slope detection and ranging (SLODAR) method to perform this task in real time from properly selected Shack-Hartmann wavefront sensor measurements accumulated over a few hundred frames from a pair of laser guide stars, thus eliminating the need for an additional instrument. The algorithm is introduced, followed by a theoretical influence function analysis illustrating its impulse response to high-resolution turbulence profiles. Finally, its performance is assessed in the context of the Thirty Meter Telescope multi-conjugate adaptive optics system via end-to-end wave optics Monte Carlo simulations.

  13. Real time microcontroller implementation of an adaptive myoelectric filter.

    PubMed

    Bagwell, P J; Chappell, P H

    1995-03-01

    This paper describes a real time digital adaptive filter for processing myoelectric signals. The filter time constant is automatically selected by the adaptation algorithm, giving a significant improvement over linear filters for estimating the muscle force and controlling a prosthetic device. Interference from mains sources often produces problems for myoelectric processing, and so 50 Hz and all harmonic frequencies are reduced by an averaging filter and differential process. This makes practical electrode placement and contact less critical and time consuming. An economic real time implementation is essential for a prosthetic controller, and this is achieved using an Intel 80C196KC microcontroller.

  14. Real-Time Optical Image Processing Techniques

    DTIC Science & Technology

    1988-10-31

    fgt modulator (LCTV SLMA" multple-focus holographic lens Jeffrey A. Davis. MEMBER SPIE ( hololens ) associative refrievot realtime robotic visWiL...above is used as an electronicallyI addressed SIM. Tswo multiple-focus holographic lenses ( hololenses ).* MHLI and MHL2. are used as space-variant optical

  15. Nonlinear real-time optical signal processing

    NASA Astrophysics Data System (ADS)

    Sawchuk, A. A.; Jenkins, B. J.

    1986-07-01

    During the period 1 July 1984 - 30 June 1985, the research under Grant AFOSR-84-0181 has concentrated on four major areas. First, work has continued on an experimental sequential optical binary parallel architecture that is constructed from an array of binary optical switching elements (NOR gates) with interconnections done by a computer-generated hologram. We are examining new binary array SLM's, high efficiency, high space-bandwidth product (SBWP) interconnection holograms, and compact reflection versions of the general architecture with the intent of building a larger demonstration system with great capabilities. Next, we have studied improved methods of providing the interconnections in these systems by the use of hybrid digital/analog (facet) holograms. We have examined analytical techniques for mapping circuit diagrams into gate locations and hologram arrays, and optimization procedures to determine the minimum set of necessary space-invariant basis functions and minimum set of space-variant indexing holograms. Another area of study has been the evaluation of devices and materials for high speed optical switching and bistability. Switching energies of 1 to 10 pJ and response times of 10 ns have been experimentally demonstrated at the University of Arizona for devices consisting of an array of Fabry-Perot cavities filled with a nonlinear material. We have begun to use the specifications of these devices and other high speed switching technologies in order to determine better designs and fundamental limits of the binary optical computing architectures under consideration.

  16. Adaptive real-time dual-comb spectroscopy

    PubMed Central

    Ideguchi, Takuro; Poisson, Antonin; Guelachvili, Guy; Picqué, Nathalie; Hänsch, Theodor W.

    2014-01-01

    The spectrum of a laser frequency comb consists of several hundred thousand equally spaced lines over a broad spectral bandwidth. Such frequency combs have revolutionized optical frequency metrology and they now hold much promise for significant advances in a growing number of applications including molecular spectroscopy. Despite an intriguing potential for the measurement of molecular spectra spanning tens of nanometres within tens of microseconds at Doppler-limited resolution, the development of dual-comb spectroscopy is hindered by the demanding stability requirements of the laser combs. Here we overcome this difficulty and experimentally demonstrate a concept of real-time dual-comb spectroscopy, which compensates for laser instabilities by electronic signal processing. It only uses free-running mode-locked lasers without any phase-lock electronics. We record spectra spanning the full bandwidth of near-infrared fibre lasers with Doppler-limited line profiles highly suitable for measurements of concentrations or line intensities. Our new technique of adaptive dual-comb spectroscopy offers a powerful transdisciplinary instrument for analytical sciences. PMID:24572636

  17. Multi-channel holographic birfurcative neural network system for real-time adaptive EOS data analysis

    NASA Technical Reports Server (NTRS)

    Liu, Hua-Kuang; Diep, J.; Huang, K.

    1991-01-01

    Viewgraphs on multi-channel holographic bifurcative neural network system for real-time adaptive Earth Observing System (EOS) data analysis are presented. The objective is to research and develop an optical bifurcating neuromorphic pattern recognition system for making optical data array comparisons and to evaluate the use of the system for EOS data classification, reduction, analysis, and other applications.

  18. Magneto-optical system for high speed real time imaging

    NASA Astrophysics Data System (ADS)

    Baziljevich, M.; Barness, D.; Sinvani, M.; Perel, E.; Shaulov, A.; Yeshurun, Y.

    2012-08-01

    A new magneto-optical system has been developed to expand the range of high speed real time magneto-optical imaging. A special source for the external magnetic field has also been designed, using a pump solenoid to rapidly excite the field coil. Together with careful modifications of the cryostat, to reduce eddy currents, ramping rates reaching 3000 T/s have been achieved. Using a powerful laser as the light source, a custom designed optical assembly, and a high speed digital camera, real time imaging rates up to 30 000 frames per seconds have been demonstrated.

  19. Magneto-optical system for high speed real time imaging.

    PubMed

    Baziljevich, M; Barness, D; Sinvani, M; Perel, E; Shaulov, A; Yeshurun, Y

    2012-08-01

    A new magneto-optical system has been developed to expand the range of high speed real time magneto-optical imaging. A special source for the external magnetic field has also been designed, using a pump solenoid to rapidly excite the field coil. Together with careful modifications of the cryostat, to reduce eddy currents, ramping rates reaching 3000 T/s have been achieved. Using a powerful laser as the light source, a custom designed optical assembly, and a high speed digital camera, real time imaging rates up to 30 000 frames per seconds have been demonstrated.

  20. Real-time control system for adaptive resonator

    SciTech Connect

    Flath, L; An, J; Brase, J; Hurd, R; Kartz, M; Sawvel, R; Silva, D

    2000-07-24

    Sustained operation of high average power solid-state lasers currently requires an adaptive resonator to produce the optimal beam quality. We describe the architecture of a real-time adaptive control system for correcting intra-cavity aberrations in a heat capacity laser. Image data collected from a wavefront sensor are processed and used to control phase with a high-spatial-resolution deformable mirror. Our controller takes advantage of recent developments in low-cost, high-performance processor technology. A desktop-based computational engine and object-oriented software architecture replaces the high-cost rack-mount embedded computers of previous systems.

  1. Adaptive Proactive Inhibitory Control for Embedded Real-Time Applications

    PubMed Central

    Yang, Shufan; McGinnity, T. Martin; Wong-Lin, KongFatt

    2012-01-01

    Psychologists have studied the inhibitory control of voluntary movement for many years. In particular, the countermanding of an impending action has been extensively studied. In this work, we propose a neural mechanism for adaptive inhibitory control in a firing-rate type model based on current findings in animal electrophysiological and human psychophysical experiments. We then implement this model on a field-programmable gate array (FPGA) prototyping system, using dedicated real-time hardware circuitry. Our results show that the FPGA-based implementation can run in real-time while achieving behavioral performance qualitatively suggestive of the animal experiments. Implementing such biological inhibitory control in an embedded device can lead to the development of control systems that may be used in more realistic cognitive robotics or in neural prosthetic systems aiding human movement control. PMID:22701420

  2. Real-Time Optical Hough Transform for Industrial Inspection

    NASA Astrophysics Data System (ADS)

    Richards, Jeffrey S.; Casasent, David P.

    1990-03-01

    We describe a real-time optical Hough transform (HT) inspection system and show quantitative inspection results using an industrial inspection application. The HT architecture uses an electronically addressed liquid crystal television (LCTV) as the real-time spatial light modulator, a novel selective edge-enhancement filtering technique, and realizes multiple slices of the HT with a computer generated hologram. The industrial case study of the inspection of cigarette packages is used to benchmark the HT processor. A test set of 100 packages is presented to the processor to qualify its effectiveness. The statistical significance of these finite test set results is also examined.

  3. Real-time optical holographic tracking of multiple objects.

    PubMed

    Chao, T H; Liu, H K

    1989-01-15

    A coherent optical correlation technique for real-time simultaneous tracking of several different objects making independent movements is described, and experimental results are presented. An evaluation of this system compared with digital computing systems is made. The real-time processing capability is obtained through the use of a liquid crystal television spatial light modulator and a dichromated gelatin multifocus hololens. A coded reference beam is utilized in the separation of the output correlation plane associated with each input target so that independent tracking can be achieved.

  4. Real-time optical holographic tracking of multiple objects

    NASA Technical Reports Server (NTRS)

    Chao, Tien-Hsin; Liu, Hua-Kuang

    1989-01-01

    A coherent optical correlation technique for real-time simultaneous tracking of several different objects making independent movements is described, and experimental results are presented. An evaluation of this system compared with digital computing systems is made. The real-time processing capability is obtained through the use of a liquid crystal television spatial light modulator and a dichromated gelatin multifocus hololens. A coded reference beam is utilized in the separation of the output correlation plane associated with each input target so that independent tracking can be achieved.

  5. Real-time Adaptive Control Using Neural Generalized Predictive Control

    NASA Technical Reports Server (NTRS)

    Haley, Pam; Soloway, Don; Gold, Brian

    1999-01-01

    The objective of this paper is to demonstrate the feasibility of a Nonlinear Generalized Predictive Control algorithm by showing real-time adaptive control on a plant with relatively fast time-constants. Generalized Predictive Control has classically been used in process control where linear control laws were formulated for plants with relatively slow time-constants. The plant of interest for this paper is a magnetic levitation device that is nonlinear and open-loop unstable. In this application, the reference model of the plant is a neural network that has an embedded nominal linear model in the network weights. The control based on the linear model provides initial stability at the beginning of network training. In using a neural network the control laws are nonlinear and online adaptation of the model is possible to capture unmodeled or time-varying dynamics. Newton-Raphson is the minimization algorithm. Newton-Raphson requires the calculation of the Hessian, but even with this computational expense the low iteration rate make this a viable algorithm for real-time control.

  6. Real-time detection of optical transients with RAPTOR

    SciTech Connect

    Borozdin, K. N.; Brumby, Steven P.; Galassi, M. C.; McGowan, K. E.; Starr, D. L.; Vestrand, W. T.; White, R. R.; Wozniak, P. R.; Wren, J.

    2002-01-01

    Fast variability of optical objects is an interesting though poorly explored subject in modern astronomy. Real-time data processing and identification of transient, celestial events in the images is very important, for such study as it allows rapid follow-up with more sensitive instruments, We discuss an approach which we have chosen for the RAPTOR project which is a pioneering close-loop system combining real-time transient detection with rapid follow-up. Our data processing pipeline is able to identify and localize an optical transient within seconds after the observation. We describe the challenges we met, solutions we found and some results obtained in our search for fast optical transients. The software pipeline we have developed for RAPTOR can easily be applied to the data from other experiments.

  7. Real-time optical processor prototype for remote SAR applications

    NASA Astrophysics Data System (ADS)

    Marchese, Linda; Doucet, Michel; Harnisch, Bernd; Suess, Martin; Bourqui, Pascal; Legros, Mathieu; Desnoyers, Nichola; Guillot, Ludovic; Mercier, Luc; Savard, Maxime; Martel, Anne; Châteauneuf, François; Bergeron, Alain

    2009-09-01

    A Compact Real-Time Optical SAR Processor has been successfully developed and tested. SAR, or Synthetic Aperture Radar, is a powerful tool providing enhanced day and night imaging capabilities. SAR systems typically generate large amounts of information generally in the form of complex data that are difficult to compress. Specifically, for planetary missions and unmanned aerial vehicle (UAV) systems with limited communication data rates this is a clear disadvantage. SAR images are typically processed electronically applying dedicated Fourier transformations. This, however, can also be performed optically in real-time. Indeed, the first SAR images have been optically processed. The optical processor architecture provides inherent parallel computing capabilities that can be used advantageously for the SAR data processing. Onboard SAR image generation would provide local access to processed information paving the way for real-time decision-making. This could eventually benefit navigation strategy and instrument orientation decisions. Moreover, for interplanetary missions, onboard analysis of images could provide important feature identification clues and could help select the appropriate images to be transmitted to Earth, consequently helping bandwidth management. This could ultimately reduce the data throughput requirements and related transmission bandwidth. This paper reviews the design of a compact optical SAR processor prototype that would reduce power, weight, and size requirements and reviews the analysis of SAR image generation using the table-top optical processor. Various SAR processor parameters such as processing capabilities, image quality (point target analysis), weight and size are reviewed. Results of image generation from simulated point targets as well as real satellite-acquired raw data are presented.

  8. Real time electrocardiogram QRS detection using combined adaptive threshold

    PubMed Central

    Christov, Ivaylo I

    2004-01-01

    Background QRS and ventricular beat detection is a basic procedure for electrocardiogram (ECG) processing and analysis. Large variety of methods have been proposed and used, featuring high percentages of correct detection. Nevertheless, the problem remains open especially with respect to higher detection accuracy in noisy ECGs Methods A real-time detection method is proposed, based on comparison between absolute values of summed differentiated electrocardiograms of one of more ECG leads and adaptive threshold. The threshold combines three parameters: an adaptive slew-rate value, a second value which rises when high-frequency noise occurs, and a third one intended to avoid missing of low amplitude beats. Two algorithms were developed: Algorithm 1 detects at the current beat and Algorithm 2 has an RR interval analysis component in addition. The algorithms are self-adjusting to the thresholds and weighting constants, regardless of resolution and sampling frequency used. They operate with any number L of ECG leads, self-synchronize to QRS or beat slopes and adapt to beat-to-beat intervals. Results The algorithms were tested by an independent expert, thus excluding possible author's influence, using all 48 full-length ECG records of the MIT-BIH arrhythmia database. The results were: sensitivity Se = 99.69 % and specificity Sp = 99.65 % for Algorithm 1 and Se = 99.74 % and Sp = 99.65 % for Algorithm 2. Conclusion The statistical indices are higher than, or comparable to those, cited in the scientific literature. PMID:15333132

  9. Real-time multi-functional optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Hyle Park, Boris; Pierce, Mark D.; Cense, Barry; de Boer, Johannes F.

    2003-04-01

    We demonstrate real-time acquisition, processing, and display of tissue structure, birefringence, and blood flow in a multi-functional optical coherence tomography (MF-OCT) system. This is accomplished by efficient data processing of the phase-resolved inteference patterns without dedicated hardware or extensive modification to the high-speed fiber-based OCT system. The system acquires images of 2048 depth scans per second, covering an area of 5 mm in width × 1.2 mm in depth with real-time display updating images in a rolling manner 32 times each second. We present a video of the system display as images from the proximal nail fold of a human volunteer are taken.

  10. Real-time automatic registration in optical surgical navigation

    NASA Astrophysics Data System (ADS)

    Lin, Qinyong; Yang, Rongqian; Cai, Ken; Si, Xuan; Chen, Xiuwen; Wu, Xiaoming

    2016-05-01

    An image-guided surgical navigation system requires the improvement of the patient-to-image registration time to enhance the convenience of the registration procedure. A critical step in achieving this aim is performing a fully automatic patient-to-image registration. This study reports on a design of custom fiducial markers and the performance of a real-time automatic patient-to-image registration method using these markers on the basis of an optical tracking system for rigid anatomy. The custom fiducial markers are designed to be automatically localized in both patient and image spaces. An automatic localization method is performed by registering a point cloud sampled from the three dimensional (3D) pedestal model surface of a fiducial marker to each pedestal of fiducial markers searched in image space. A head phantom is constructed to estimate the performance of the real-time automatic registration method under four fiducial configurations. The head phantom experimental results demonstrate that the real-time automatic registration method is more convenient, rapid, and accurate than the manual method. The time required for each registration is approximately 0.1 s. The automatic localization method precisely localizes the fiducial markers in image space. The averaged target registration error for the four configurations is approximately 0.7 mm. The automatic registration performance is independent of the positions relative to the tracking system and the movement of the patient during the operation.

  11. Real-Time Adaptive Color Segmentation by Neural Networks

    NASA Technical Reports Server (NTRS)

    Duong, Tuan A.

    2004-01-01

    Artificial neural networks that would utilize the cascade error projection (CEP) algorithm have been proposed as means of autonomous, real-time, adaptive color segmentation of images that change with time. In the original intended application, such a neural network would be used to analyze digitized color video images of terrain on a remote planet as viewed from an uninhabited spacecraft approaching the planet. During descent toward the surface of the planet, information on the segmentation of the images into differently colored areas would be updated adaptively in real time to capture changes in contrast, brightness, and resolution, all in an effort to identify a safe and scientifically productive landing site and provide control feedback to steer the spacecraft toward that site. Potential terrestrial applications include monitoring images of crops to detect insect invasions and monitoring of buildings and other facilities to detect intruders. The CEP algorithm is reliable and is well suited to implementation in very-large-scale integrated (VLSI) circuitry. It was chosen over other neural-network learning algorithms because it is better suited to realtime learning: It provides a self-evolving neural-network structure, requires fewer iterations to converge and is more tolerant to low resolution (that is, fewer bits) in the quantization of neural-network synaptic weights. Consequently, a CEP neural network learns relatively quickly, and the circuitry needed to implement it is relatively simple. Like other neural networks, a CEP neural network includes an input layer, hidden units, and output units (see figure). As in other neural networks, a CEP network is presented with a succession of input training patterns, giving rise to a set of outputs that are compared with the desired outputs. Also as in other neural networks, the synaptic weights are updated iteratively in an effort to bring the outputs closer to target values. A distinctive feature of the CEP neural

  12. Real-time edge-enhanced optical correlator

    NASA Technical Reports Server (NTRS)

    Liu, Tsuen-Hsi (Inventor); Cheng, Li-Jen (Inventor)

    1992-01-01

    Edge enhancement of an input image by four-wave mixing a first write beam with a second write beam in a photorefractive crystal, GaAs, was achieved for VanderLugt optical correlation with an edge enhanced reference image by optimizing the power ratio of a second write beam to the first write beam (70:1) and optimizing the power ratio of a read beam, which carries the reference image to the first write beam (100:701). Liquid crystal TV panels are employed as spatial light modulators to change the input and reference images in real time.

  13. Real-time optical correlator using photorefractive GaAs

    NASA Technical Reports Server (NTRS)

    Liu, Duncan T. H.; Cheng, Li-Jen; Chao, Tien-Hsin; Yu, Jeffrey W.; Gregory, Don A.

    1990-01-01

    A real-time optical correlator based on GaAs and liquid-crystal TV (LCTV) is demonstrated. The demonstrated system has a video-frame rate limited by the speed of the LCTVs; if faster spatial-light modulators are used, the potential frame rate of a GaAs-based correlator can be as fast as 1000 frames/sec under experimental conditions. Comparisons are made between VanderLugt and joint transform and between degenerate and nondegenerate four-wave mixing. The edge-enhancement effect and the Bragg diffraction effect are discussed.

  14. Real-time dosimetry in radiotherapy using tailored optical fibers

    NASA Astrophysics Data System (ADS)

    Rahman, A. K. M. Mizanur; Zubair, H. T.; Begum, Mahfuza; Abdul-Rashid, H. A.; Yusoff, Z.; Omar, Nasr Y. M.; Ung, N. M.; Mat-Sharif, K. A.; Bradley, D. A.

    2016-05-01

    Real-time dosimetry plays an important role for accurate patient-dose measurement during radiotherapy. A tiny piece of laboratory fabricated Ge-doped optical fiber has been investigated as a radioluminescence (RL) sensor for real-time dosimetry over the dose range from 1 Gy to 8 Gy under 6 MV photon beam by LINAC. Fiber-coupled software-based RL prototype system was used to assess essential dosimetric characteristics including dose response linearity, dose rate dependency, sensitivity, repeatability and output dependence on field sizes. The consistency level of RL photon counts versus dose rate was also compared with that of standard Al2O3:C chips. Sensitivity of Ge-doped fiber were found to be sufficiently sensitive for practical use and also provided linear dose responses for various dose rates from 100 cGy/min to 600 cGy/min using both 6 MV photon and 6 MeV electron beams. SEM-EDX analysis was performed to identify Ge-dopant concentration level within the optical fiber RL material. Accumulated doses were also estimated using simple integral technique and the error was found to be around less than 1% under dissimilar dose rates or repeat measurements. The evaluation of the Ge-doped optical fiber based RL dosimeter system indicates its potential in medical dosimetry.

  15. Hybrid integrated optic modules for real-time signal processing

    NASA Technical Reports Server (NTRS)

    Tsai, C. S.

    1984-01-01

    The most recent progress on four relatively new hybrid integrated optic device modules in LiNbO3 waveguides and one in YIG/GGG waveguide that are currently being studied are discussed. The five hybrid modules include a time-integrating acoustooptic correlator, a channel waveguide acoustooptic frequency shifter/modulator, an electrooptic channel waveguide total internal reflection moculator/switch, an electrooptic analog-to-digital converter using a Fabry-Perot modulator array, and a noncollinear magnetooptic modulator using magnetostatic surface waves. All of these devices possess the desirable characteristics of very large bandwidth (GHz or higher), very small substrate size along the optical path (typically 1.5 cm or less), single-mode optical propagation, and low drive power requirement. The devices utilize either acoustooptic, electrooptic or magnetooptic effects in planar or channel waveguides and, therefore, act as efficient interface devices between a light wave and temporal signals. Major areas of application lie in wideband multichannel optical real-time signal processing and communications. Some of the specific applications include spectral analysis and correlation of radio frequency (RF) signals, fiber-optic sensing, optical computing and multiport switching/routing, and analog-to-digital conversion of wide RF signals.

  16. Multi-layer holographic bifurcative neural network system for real-time adaptive EOS data analysis

    NASA Technical Reports Server (NTRS)

    Liu, Hua-Kuang; Huang, K. S.; Diep, J.

    1993-01-01

    Optical data processing techniques have the inherent advantage of high data throughout, low weight and low power requirements. These features are particularly desirable for onboard spacecraft in-situ real-time data analysis and data compression applications. the proposed multi-layer optical holographic neural net pattern recognition technique will utilize the nonlinear photorefractive devices for real-time adaptive learning to classify input data content and recognize unexpected features. Information can be stored either in analog or digital form in a nonlinear photofractive device. The recording can be accomplished in time scales ranging from milliseconds to microseconds. When a system consisting of these devices is organized in a multi-layer structure, a feedforward neural net with bifurcating data classification capability is formed. The interdisciplinary research will involve the collaboration with top digital computer architecture experts at the University of Southern California.

  17. Multi-layer holographic bifurcative neural network system for real-time adaptive EOS data analysis

    NASA Technical Reports Server (NTRS)

    Liu, Hua-Kuang; Huang, K.; Diep, J.

    1992-01-01

    Optical data processing techniques have the inherent advantage of high data throughout, low weight and low power requirements. These features are particularly desirable for onboard spacecraft in-situ real-time data analysis and data compression applications. The proposed multi-layer optical holographic neural net pattern recognition technique will utilize the nonlinear photorefractive devices for real-time adaptive learning to classify input data content and recognize unexpected features. Information can be stored either in analog or digital form in a nonlinear photorefractive device. The recording can be accomplished in time scales ranging from milliseconds to microseconds. When a system consisting of these devices is organized in a multi-layer structure, a feed forward neural net with bifurcating data classification capability is formed. The interdisciplinary research will involve the collaboration with top digital computer architecture experts at the University of Southern California.

  18. Resource Management for Real-Time Adaptive Agents

    NASA Technical Reports Server (NTRS)

    Welch, Lonnie; Chelberg, David; Pfarr, Barbara; Fleeman, David; Parrott, David; Tan, Zhen-Yu; Jain, Shikha; Drews, Frank; Bruggeman, Carl; Shuler, Chris

    2003-01-01

    Increased autonomy and automation in onboard flight systems offer numerous potential benefits, including cost reduction and greater flexibility. The existence of generic mechanisms for automation is critical for handling unanticipated science events and anomalies where limitations in traditional control software with fixed, predetermined algorithms can mean loss of science data and missed opportunities for observing important terrestrial events. We have developed such a mechanism by adding a Hierarchical Agent-based ReaLTime technology (HART) extension to our Dynamic Resource Management (DRM) middleware. Traditional DRM provides mechanisms to monitor the realtime performance of distributed applications and to move applications among processors to improve real-time performance. In the HART project we have designed and implemented a performance adaptation mechanism to improve reaktime performance. To use this mechanism, applications are developed that can run at various levels of quality. The DRM can choose a setting for the quality level of an application dynamically at run-time in order to manage satellite resource usage more effectively. A groundbased prototype of a satellite system that captures and processes images has also been developed as part of this project to be used as a benchmark for evaluating the resource management framework A significant enhancement of this generic mission-independent framework allows scientists to specify the utility, or "scientific benefit," of science observations under various conditions like cloud cover and compression method. The resource manager then uses these benefit tables to determine in redtime how to set the quality levels for applications to maximize overall system utility as defined by the scientists running the mission. We also show how maintenance functions llke health and safety data can be integrated into the utility framework. Once thls framework has been certified for missions and successfully flight tested it

  19. Real-time Optimization of an Ion Optical Beamline

    NASA Astrophysics Data System (ADS)

    Schillaci, Zachary; Amthor, Matthew; Morrissey, Dave; Portillo, Mauricio; Schwarz, Stefan; Steiner, Mathias; Sumithrarachchi, Chandana

    2015-10-01

    We have developed an experimental approach to automatically adjust multiple electrostatic and/or magnetic elements on an ion optical beamline, while analyzing the profile of the beam on a detector at the image point, until an optimal tune is found. This approach dramatically simplifies beamline tuning, thus allowing more efficient use of experimental equipment; ensures a more optimal tune is found, providing a more focused beam spot without a significant loss of beam transmission; and will allow the development of specialized optical tunes based on the needs of any given experiment. The approach was tested directly on the D-Line at the National Superconducting Cyclotron Laboratory at Michigan State University in several real-time optimization runs. The initial experiments demonstrate the ability of the optimizer to focus the beam while preserving transmission, ultimately halving σx and σy of the beam spot within a one-hour optimization run relative to that produced through a manual tweak of a model based tune. With further research we plan to generalize the approach to work on any given beamline, including particularly for higher order tunes of fragment separators. NSF REU Grant #PHY-1156964 and NSF Grant #PHY-1102511.

  20. Acousto-optic/CCD real-time SAR data processor

    NASA Technical Reports Server (NTRS)

    Psaltis, D.

    1983-01-01

    The SAR processor which uses an acousto-optic device as the input electronic-to-optical transducer and a 2-D CCD image sensor, which is operated in the time-delay-and-integrate (TDI) mode is presented. The CCD serves as the optical detector, and it simultaneously operates as an array of optically addressed correlators. The lines of the focused SAR image form continuously (at the radar PRF) at the final row of the CCD. The principles of operation of this processor, its performance characteristics, the state-of-the-art of the devices used and experimental results are outlined. The methods by which this processor can be made flexible so that it can be dynamically adapted to changing SAR geometries is discussed.

  1. Real-time optimal sensing strategies for active control of optical systems

    NASA Astrophysics Data System (ADS)

    Moon, Suk-Min; Fowler, Leslie P.; Clark, Robert L.; Anderson, Eric H.

    2007-04-01

    The pointing and imaging performance of precision optical systems is degraded by disturbances on the system that create optical jitter. These disturbances can be caused by structural motion of optical components due to vibration sources that (1) originate within the optical system, (2) originate external to the system and are transmitted through the structural path in the environment, and (3) are air-induced vibrations from acoustic noise. Beam control systems can suppress optical jitter, and active control techniques can be used to extend performance by incorporating information from accelerometers, microphones, and other auxiliary sensors. In some applications, offline fixed gain controllers can be used to minimize jitter. However there are many applications in which a real-time adaptive control approach would yield improved optical performance. Often we would like the capability to adapt in real-time to a system which is time-varying or whose disturbances are non-stationary and hard to predict. In the presence of these harsh, ever-changing environments we would like to use every available tool to optimize performance. Improvements in control algorithms are important, but another potentially useful tool is a real-time adaptive control method employing optimal sensing strategies. In this approach, real-time updating of reference sensors is provided to minimize optical jitter. The technique selects an optimal subset of sensors to use as references from an array of possible sensor locations. The optimal, weighted reference sensor set is well correlated with the disturbance and when used with an adaptive control algorithm, results in improved line-of-sight jitter performance with less computational burden compared to a controller which uses multiple reference sensors. The proposed technique is applied to an experimental test bed in which multiple proof-mass actuators generate structural vibrations on a flexible plate. These vibrations are transmitted to an optical

  2. Real-Time Optical Correlator Based On GaAs

    NASA Technical Reports Server (NTRS)

    Liu, Tsuen-Hsi; Cheng, Li-Jen

    1992-01-01

    Apparatus performs correlation between input image and reference image in real time by means of degenerate four-wave mixing in photorefractive crystal, which serves as real-time holographic medium. Gallium arsenide chosen to be photorefractive material in this application because at frame rate and level of illumination used in experiments, offers adequate diffraction efficiency. Frame rates as high as 1,000 s to negative 1st power achievable.

  3. Experimental demonstration of real-time adaptive one-qubit quantum-state tomography

    NASA Astrophysics Data System (ADS)

    Yin, Qi; Li, Li; Xiang, Xiao; Xiang, Guo-Yong; Li, Chuang-Feng; Guo, Guang-Can

    2017-01-01

    Quantum-state tomography plays a pivotal role in quantum computation and information processing. To improve the accuracy in estimating an unknown state, carefully designed measurement schemes, such as adopting an adaptive strategy, are necessarily needed, which have gained great interest recently. In this work, based on the proposal of Sugiyama et al. [Phys. Rev. A 85, 052107 (2012)], 10.1103/PhysRevA.85.052107, we experimentally realize an adaptive quantum-state tomography for one qubit in an optical system. Since this scheme gives an analytical solution to the optimal measurement basis problem, our experiment is updated in real time and the infidelity between the real state and the estimated state is tracked with the detected photons. We observe an almost 1 /N scaling rule of averaged infidelity against the overall number of photons, N , in our experiment, which outperforms 1 /√{N } of nonadaptive schemes.

  4. Real-Time Adaptive Least-Squares Drag Minimization for Performance Adaptive Aeroelastic Wing

    NASA Technical Reports Server (NTRS)

    Ferrier, Yvonne L.; Nguyen, Nhan T.; Ting, Eric

    2016-01-01

    This paper contains a simulation study of a real-time adaptive least-squares drag minimization algorithm for an aeroelastic model of a flexible wing aircraft. The aircraft model is based on the NASA Generic Transport Model (GTM). The wing structures incorporate a novel aerodynamic control surface known as the Variable Camber Continuous Trailing Edge Flap (VCCTEF). The drag minimization algorithm uses the Newton-Raphson method to find the optimal VCCTEF deflections for minimum drag in the context of an altitude-hold flight control mode at cruise conditions. The aerodynamic coefficient parameters used in this optimization method are identified in real-time using Recursive Least Squares (RLS). The results demonstrate the potential of the VCCTEF to improve aerodynamic efficiency for drag minimization for transport aircraft.

  5. Real-time phase-resolved functional optical coherence tomography by use of optical Hilbert transformation

    NASA Astrophysics Data System (ADS)

    Zhao, Yonghua; Chen, Zhongping; Ding, Zhihua; Ren, Hongwu; Nelson, J. Stuart

    2002-01-01

    We have developed a novel real-time phase-resolved functional optical coherence tomography system that uses optical Hilbert transformation. When we use a resonant scanner in the reference arm of the interferometer, with an axial scanning speed of 4 kHz, the frame rate of both structural and Doppler blood-flow imaging with a size of 100 by 100 pixels is 10 Hz. The system has high sensitivity and a larger dynamic range for measuring the Doppler frequency shift that is due to moving red blood cells. Real-time images of in vivo blood flow in human skin obtained with this interferometer are presented.

  6. Real-time optical laboratory solution of parabolic differential equations

    NASA Technical Reports Server (NTRS)

    Casasent, David; Jackson, James

    1988-01-01

    An optical laboratory matrix-vector processor is used to solve parabolic differential equations (the transient diffusion equation with two space variables and time) by an explicit algorithm. This includes optical matrix-vector nonbase-2 encoded laboratory data, the combination of nonbase-2 and frequency-multiplexed data on such processors, a high-accuracy optical laboratory solution of a partial differential equation, new data partitioning techniques, and a discussion of a multiprocessor optical matrix-vector architecture.

  7. Optical, real-time monitoring of the glomerular filtration rate

    NASA Astrophysics Data System (ADS)

    Rabito, Carlos A.; Chen, Yang; Schomacker, Kevin T.; Modell, Mark D.

    2005-10-01

    An easy and accurate assessment of the renal function is a critical requirement for detecting the initial functional decline of the kidney induced by acute or chronic renal disease. A method for measuring the glomerular filtration rate is developed with the accuracy of clearance techniques and the convenience of plasma creatinine. The renal function is measured in rats as the rate of clearance determined from time-resolved transcutaneous fluorescence measurements of a new fluorescent glomerular filtration agent. The agent has a large dose-safety coefficient and the same space distribution and clearance characteristics as iothalamate. This new approach is a convenient and accurate way to perform real-time measurements of the glomerular filtration rate to detect early kidney disease before the renal function becomes severely and irreversibly compromised.

  8. Preconditioning of real-time optical Wiener filters for array processing

    NASA Astrophysics Data System (ADS)

    Ghosh, Anjan; Paparao, Palacharla

    1992-07-01

    In adaptive array processors, a performance measure, such as mean square error or signal to noise ration, coverages to the optimum Wiener solution starting from an initial setting. The choice of adaptive algorithms to solve the Wiener filtering problem is mainly guided by the desired processing time. In an optical realization for direct calculation of the optimum weights the covariance matrix and vector for a Wiener filter are computed at a high speed on acousto-optic processors. The resulting linear system of equations can be solved on an iterative optical processor. The matrix and vector data should be recomputed in every iteration for better tracking and adaptation. This introduces variations in their values due to the time-varying jamming and interference noise and the optical errors and noise. Time variant steepest descent algorithm is a simple method that converges to the common solution. In this paper, we describe a real-time preconditioning technique for such nonstationary iterative methods. Preconditioning will progressively lower the condition number of each matrix in the sequence, thereby improving the convergence speed and accuracy of the solution. This preconditioning process involves matrix-matrix multiplications that can be performed at high speed on parallel optical processors. Results of simulations illustrate the superlinear convergence obtained from preconditioning.

  9. Real-time holographic compensation of large optics for deployment in space

    NASA Astrophysics Data System (ADS)

    Guthals, Dennis M.; Sox, Daniel; Joswick, Michael D.; Rodney, Paul J.

    2000-11-01

    Large deployable space-based optical systems will likely require complex structure position controls in conjunction with an adaptive optic to maintain optical tolerances necessary for near diffraction-limited performance. A real- time holographic (RTH) compensation system can greatly reduce the requirements and complexity of the position control system and enable the use of novel or imperfect optical components for large mirror surfaces. A hologram of the distorted primary is recorded with a local beacon at 532 nm (approximately 100 nJ/exposure) on an optically addressed spatial light modulator and transferred as a phase grating to a ferroelectric liquid crystal layer. The hologram is played back with target light containing the same optical distortion. A corrected image is obtained in the conjugate diffracted order where the phase of the optical distortion is subtracted from the distorted image. We report recent test results and analysis of a RTH- compensated deformed mirror of 0.75 m diameter. The short exposure hologram is recorded at video frequencies (30 Hz) at bandwidths up to 5 kHz. Correction for tens of waves of static and dynamic optical distortions including mechanical and thermal warp, mechanical vibration, and air turbulence are shown for monochromatic (532 nm) and broadband (532 +/- 40 nm) illuminated targets. We also report on the generation of blazed gratings in electrically addressed spatial light modulators to achieve greatly enhanced diffraction efficiencies and to perform beam steering.

  10. Fiber Optics Deliver Real-Time Structural Monitoring

    NASA Technical Reports Server (NTRS)

    2013-01-01

    To alter the shape of aircraft wings during flight, researchers at Dryden Flight Research Center worked on a fiber optic sensor system with Austin-based 4DSP LLC. The company has since commercialized a new fiber optic system for monitoring applications in health and medicine, oil and gas, and transportation, increasing company revenues by 60 percent.

  11. A Novel Hand-Held Optical Imager with Real-Time Coregistration Facilities Toward Diagnostic Mammography

    DTIC Science & Technology

    2011-01-01

    1-0004 TITLE: A Novel Hand-Held Optical Imager with Real-Time Coregistration Facilities toward Diagnostic Mammography PRINCIPAL...Summary 3. DATES COVERED (From - To) 1 Jan 2010 – 31 Dec 2010 4. TITLE AND SUBTITLE A Novel Hand-held Optical Imager with Real-Time Coregistration ...translation of a hand-held optical imager with automated coregistration facilities toward 3D tomography. Studies were performed in vivo with healthy female

  12. A Real-Time Optical Tracking and Measurement Processing System for Flying Targets

    PubMed Central

    Guo, Pengyu; Ding, Shaowen; Zhang, Hongliang; Zhang, Xiaohu

    2014-01-01

    Optical tracking and measurement for flying targets is unlike the close range photography under a controllable observation environment, which brings extreme conditions like diverse target changes as a result of high maneuver ability and long cruising range. This paper first designed and realized a distributed image interpretation and measurement processing system to achieve resource centralized management, multisite simultaneous interpretation and adaptive estimation algorithm selection; then proposed a real-time interpretation method which contains automatic foreground detection, online target tracking, multiple features location, and human guidance. An experiment is carried out at performance and efficiency evaluation of the method by semisynthetic video. The system can be used in the field of aerospace tests like target analysis including dynamic parameter, transient states, and optical physics characteristics, with security control. PMID:24987748

  13. Real-time digital signal processing for live electro-optic imaging.

    PubMed

    Sasagawa, Kiyotaka; Kanno, Atsushi; Tsuchiya, Masahiro

    2009-08-31

    We present an imaging system that enables real-time magnitude and phase detection of modulated signals and its application to a Live Electro-optic Imaging (LEI) system, which realizes instantaneous visualization of RF electric fields. The real-time acquisition of magnitude and phase images of a modulated optical signal at 5 kHz is demonstrated by imaging with a Si-based high-speed CMOS image sensor and real-time signal processing with a digital signal processor. In the LEI system, RF electric fields are probed with light via an electro-optic crystal plate and downconverted to an intermediate frequency by parallel optical heterodyning, which can be detected with the image sensor. The artifacts caused by the optics and the image sensor characteristics are corrected by image processing. As examples, we demonstrate real-time visualization of electric fields from RF circuits.

  14. A non-optical real-time particle fallout monitor

    NASA Technical Reports Server (NTRS)

    Chuan, Raymond L.; Bowers, William D.

    1990-01-01

    The paper describes a size-selective fallout monitor that can be employed to assess the degradation of sensitive optical surfaces as well as facilitate the analysis of particle types. The device combines a vertical elutriator and a quartz crystal microbalance, and only particles greater than a specific size can pass through an upward laminar flow generated in the device. The larger particles cause a frequency shift in the crystal oscillator, thereby permitting the measurement of the fallout associated with the contamination of optical instruments.

  15. Real-time processing for Fourier domain optical coherence tomography using a field programmable gate array

    PubMed Central

    Ustun, Teoman E.; Iftimia, Nicusor V.; Ferguson, R. Daniel; Hammer, Daniel X.

    2008-01-01

    Real-time display of processed Fourier domain optical coherence tomography (FDOCT) images is important for applications that require instant feedback of image information, for example, systems developed for rapid screening or image-guided surgery. However, the computational requirements for high-speed FDOCT image processing usually exceeds the capabilities of most computers and therefore display rates rarely match acquisition rates for most devices. We have designed and developed an image processing system, including hardware based upon a field programmable gated array, firmware, and software that enables real-time display of processed images at rapid line rates. The system was designed to be extremely flexible and inserted in-line between any FDOCT detector and any Camera Link frame grabber. Two versions were developed for spectrometer-based and swept source-based FDOCT systems, the latter having an additional custom high-speed digitizer on the front end but using all the capabilities and features of the former. The system was tested in humans and monkeys using an adaptive optics retinal imager, in zebrafish using a dual-beam Doppler instrument, and in human tissue using a swept source microscope. A display frame rate of 27 fps for fully processed FDOCT images (1024 axial pixels×512 lateral A-scans) was achieved in the spectrometer-based systems. PMID:19045902

  16. Real-time processing for Fourier domain optical coherence tomography using a field programmable gate array

    NASA Astrophysics Data System (ADS)

    Ustun, Teoman E.; Iftimia, Nicusor V.; Ferguson, R. Daniel; Hammer, Daniel X.

    2008-11-01

    Real-time display of processed Fourier domain optical coherence tomography (FDOCT) images is important for applications that require instant feedback of image information, for example, systems developed for rapid screening or image-guided surgery. However, the computational requirements for high-speed FDOCT image processing usually exceeds the capabilities of most computers and therefore display rates rarely match acquisition rates for most devices. We have designed and developed an image processing system, including hardware based upon a field programmable gated array, firmware, and software that enables real-time display of processed images at rapid line rates. The system was designed to be extremely flexible and inserted in-line between any FDOCT detector and any Camera Link frame grabber. Two versions were developed for spectrometer-based and swept source-based FDOCT systems, the latter having an additional custom high-speed digitizer on the front end but using all the capabilities and features of the former. The system was tested in humans and monkeys using an adaptive optics retinal imager, in zebrafish using a dual-beam Doppler instrument, and in human tissue using a swept source microscope. A display frame rate of 27 fps for fully processed FDOCT images (1024 axial pixels×512 lateral A-scans) was achieved in the spectrometer-based systems.

  17. Near real time accurate bacterial enumeration in aquatic environment using an all-fibre optical system

    NASA Astrophysics Data System (ADS)

    Bogomolny, E.; Swift, S.; Patel, A.; Cheng, M.; Vanholsbeeck, F.

    2013-06-01

    We developed a computerized optical probe for near real time bacterial detection in water. This microorganism detection technique, based on fluorescence enhanced by nucleic acids staining, shows promising results compared to conventional methods.

  18. Real-time damage assessment using fiber optic grating sensors

    NASA Astrophysics Data System (ADS)

    Calvert, Sean G.; Conte, Joel P.; Moaveni, Babak; Schulz, Whitten L.; de Callafon, Raymond

    2003-11-01

    Over the past few years Blue Road Research and the University of California at San Diego have been collaborating to develop a bridge health monitoring system using long gage length fiber optic strain sensors and modal analysis. Two programs supporting this effort have been funded by the National Science Foundation and from this work several papers have been published showing its strong progress1-5. In 2002, the Federal Highway Administration and Caltrans performed a full-scale test on some of the components that will be used for the planned I-5/Gilman Advanced technology Bridge in California, USA. As a part of this test Blue Road Research used its developmental system to validate the use of this damage detection technique and to compare the results with conventional modal analysis tools.

  19. Real-time Optical Network for Accelerator Control

    SciTech Connect

    Lee, Keun

    2012-06-27

    The timing requirements of a modern accelerator complex call for several features. The first is a system for high precision relative timing among accelerator components. Stabilized fiber links have already been demonstrated to achieve sub-10 femtoseconds relative timing precision. The second is a system for timing distribution of absolute time with sufficient precision to identify a specific RF bucket. The White Rabbit technology is a promising candidate to deliver the absolute time that is linked to the GPS clock. In this study we demonstrated that these two technologies can be combined in a way that the absolute time information can be delivered to the stabilized fiber link system. This was accomplished by researching the design of the stabilized fiber and White Rabbit systems and devising adaptation modules that facilitate co-existence of both systems in the same FPGA environment. We built a prototype system using off-the-shelf products and implemented a proof-of-concept version of the FPGA firmware. The test verified that the White Rabbit features operate correctly under the stabilized fiber system environment. This work demonstrates that turn-key femtosecond timing systems with absolute time information can be built cost effectively and deployed in various accelerator environments. This will lead to many new applications in chemistry, biology and surface dynamics, to name a few.

  20. Remote mission specialist - A study in real-time, adaptive planning

    NASA Technical Reports Server (NTRS)

    Rokey, Mark J.

    1990-01-01

    A high-level planning architecture for robotic operations is presented. The remote mission specialist integrates high-level directives with low-level primitives executable by a run-time controller for command of autonomous servicing activities. The planner has been designed to address such issues as adaptive plan generation, real-time performance, and operator intervention.

  1. Computing the total atmospheric refraction for real-time optical imaging sensor simulation

    NASA Astrophysics Data System (ADS)

    Olson, Richard F.

    2015-05-01

    Fast and accurate computation of light path deviation due to atmospheric refraction is an important requirement for real-time simulation of optical imaging sensor systems. A large body of existing literature covers various methods for application of Snell's Law to the light path ray tracing problem. This paper provides a discussion of the adaptation to real time simulation of atmospheric refraction ray tracing techniques used in mid-1980's LOWTRAN releases. The refraction ray trace algorithm published in a LOWTRAN-6 technical report by Kneizys (et. al.) has been coded in MATLAB for development, and in C-language for simulation use. To this published algorithm we have added tuning parameters for variable path segment lengths, and extensions for Earth grazing and exoatmospheric "near Earth" ray paths. Model atmosphere properties used to exercise the refraction algorithm were obtained from tables published in another LOWTRAN-6 related report. The LOWTRAN-6 based refraction model is applicable to atmospheric propagation at wavelengths in the IR and visible bands of the electromagnetic spectrum. It has been used during the past two years by engineers at the U.S. Army Aviation and Missile Research, Development and Engineering Center (AMRDEC) in support of several advanced imaging sensor simulations. Recently, a faster (but sufficiently accurate) method using Gauss-Chebyshev Quadrature integration for evaluating the refraction integral was adopted.

  2. Simple all-optical FFT scheme enabling Tbit/s real-time signal processing.

    PubMed

    Hillerkuss, D; Winter, M; Teschke, M; Marculescu, A; Li, J; Sigurdsson, G; Worms, K; Ben Ezra, S; Narkiss, N; Freude, W; Leuthold, J

    2010-04-26

    A practical scheme to perform the fast Fourier transform in the optical domain is introduced. Optical real-time FFT signal processing is performed at speeds far beyond the limits of electronic digital processing, and with negligible energy consumption. To illustrate the power of the method we demonstrate an optical 400 Gbit/s OFDM receiver. It performs an optical real-time FFT on the consolidated OFDM data stream, thereby demultiplexing the signal into lower bit rate subcarrier tributaries, which can then be processed electronically.

  3. Priority-progress CPU adaptation for elastic real-time applications

    NASA Astrophysics Data System (ADS)

    Krasic, Charles; Sinha, Anirban; Kirsh, Lowell

    2007-01-01

    As multimedia-capable, network-enabled devices become ever more abundant, device heterogeneity and resource sharing dynamics remain difficult challenges in networked continuous media applications. These challenges often cause the applications to exhibit very brittle real-time performance. Due to heterogeneity, minor variations in encoding can mean a continuous media item performs well on some devices but very poorly on others. Resource sharing can mean that content can work for some of the time, but real-time delivery is frequently interrupted due to competition for resources. Quality-adaptive approaches seek to preserve real-time performance, by evaluating and executing trade-offs between the quality of application results and the resources required and available to produce them. Since the approach requires the applications to adapt the results they produce, we refer to them as elastic real-time applications. In this paper, we use video as a specific example of an elastic real-time application. We describe a general strategy for CPU adaptation called Priority-Progress adaptation, which compliments and extends previous work on adaptation for network bandwidth. The basic idea of Priority-Progress is to utilize priority and timestamp attributes of the media to reorder execution steps, so that low priority work can be skipped in the event that the CPU is too constrained to otherwise maintain real-time progress. We have implemented this approach in a prototype video application. We will present benchmark results that demonstrate the advantages of Priority-Progress adaptation in comparison to techniques employed in current popular video players. These advantages include better timeliness as CPU utilization approaches saturation, and more user-centric control over quality-adapation (for example to boost the video quality of selected video in a multi-video scenario). Although we focus on video in this paper, we believe that the Priority-Progress technique is applicable to

  4. The GFZ real-time GNSS precise positioning service system and its adaption for COMPASS

    NASA Astrophysics Data System (ADS)

    Li, Xingxing; Ge, Maorong; Zhang, Hongping; Nischan, Thomas; Wickert, Jens

    2013-03-01

    Motivated by the IGS real-time Pilot Project, GFZ has been developing its own real-time precise positioning service for various applications. An operational system at GFZ is now broadcasting real-time orbits, clocks, global ionospheric model, uncalibrated phase delays and regional atmospheric corrections for standard PPP, PPP with ambiguity fixing, single-frequency PPP and regional augmented PPP. To avoid developing various algorithms for different applications, we proposed a uniform algorithm and implemented it into our real-time software. In the new processing scheme, we employed un-differenced raw observations with atmospheric delays as parameters, which are properly constrained by real-time derived global ionospheric model or regional atmospheric corrections and by the empirical characteristics of the atmospheric delay variation in time and space. The positioning performance in terms of convergence time and ambiguity fixing depends mainly on the quality of the received atmospheric information and the spatial and temporal constraints. The un-differenced raw observation model can not only integrate PPP and NRTK into a seamless positioning service, but also syncretize these two techniques into a unique model and algorithm. Furthermore, it is suitable for both dual-frequency and sing-frequency receivers. Based on the real-time data streams from IGS, EUREF and SAPOS reference networks, we can provide services of global precise point positioning (PPP) with 5-10 cm accuracy, PPP with ambiguity-fixing of 2-5 cm accuracy, PPP using single-frequency receiver with accuracy of better than 50 cm and PPP with regional augmentation for instantaneous ambiguity resolution of 1-3 cm accuracy. We adapted the system for current COMPASS to provide PPP service. COMPASS observations from a regional network of nine stations are used for precise orbit determination and clock estimation in simulated real-time mode, the orbit and clock products are applied for real-time precise point

  5. Performance evaluation of gratings applied by genetic algorithm for the real-time optical interconnection

    NASA Astrophysics Data System (ADS)

    Yoon, Jin-Seon; Kim, Nam; Suh, HoHyung; Jeon, Seok Hee

    2000-03-01

    In this paper, gratings to apply for the optical interconnection are designed using a genetic algorithm (GA) for a robust and efficient schema. The real-time optical interconnection system architecture is composed with LC-SLM, CCD array detector, IBM-PC, He-Ne laser, and Fourier transform lens. A pixelated binary phase grating is displayed on LC-SLM and could interconnect incoming beams to desired output spots freely by real-time. So as to adapt a GA for finding near globally-cost solutions, a chromosome is coded as a binary integer of length 32 X 32, the stochastic tournament method for decreasing the stochastic sampling error is performed, and a single-point crossover having 16 X 16 block size is used. The characteristics on the several parameters are analyzed in the desired grating design. Firstly, as the analysis of the effect on the probability of crossover, a designed grating when the probability of crossover is 0.75 has a 74.7[%] high diffraction efficiency and a 1.73 X 10-1 uniformity quantitatively, where the probability of mutation is 0.001 and the population size is 300. Secondly, on the probability of mutation, a designed grating when the probability of mutation is 0.001 has a 74.4[%] high efficiency and a 1.61 X 10-1 uniformity quantitatively, where the probability of crossover is 1.0 and the population size is 300. Thirdly, on the population size, a designed grating when the population size is 300 and the generation is 400 has above 74[%] diffraction efficiency, where the probability of mutation is 0.001 and the probability of crossover is 1.0.

  6. Real-time restoration of white-light confocal microscope optical sections

    PubMed Central

    Balasubramanian, Madhusudhanan; Iyengar, S. Sitharama; Beuerman, Roger W.; Reynaud, Juan; Wolenski, Peter

    2009-01-01

    Confocal microscopes (CM) are routinely used for building 3-D images of microscopic structures. Nonideal imaging conditions in a white-light CM introduce additive noise and blur. The optical section images need to be restored prior to quantitative analysis. We present an adaptive noise filtering technique using Karhunen–Loéve expansion (KLE) by the method of snapshots and a ringing metric to quantify the ringing artifacts introduced in the images restored at various iterations of iterative Lucy–Richardson deconvolution algorithm. The KLE provides a set of basis functions that comprise the optimal linear basis for an ensemble of empirical observations. We show that most of the noise in the scene can be removed by reconstructing the images using the KLE basis vector with the largest eigenvalue. The prefiltering scheme presented is faster and does not require prior knowledge about image noise. Optical sections processed using the KLE prefilter can be restored using a simple inverse restoration algorithm; thus, the methodology is suitable for real-time image restoration applications. The KLE image prefilter outperforms the temporal-average prefilter in restoring CM optical sections. The ringing metric developed uses simple binary morphological operations to quantify the ringing artifacts and confirms with the visual observation of ringing artifacts in the restored images. PMID:20186290

  7. Real-Time and Memory Correlation via Acousto-Optic Processing,

    DTIC Science & Technology

    1978-06-01

    acousto - optic technology as an answer to these requirements appears very attractive. Three fundamental signal-processing schemes using the acousto ... optic interaction have been investigated: (i) real-time correlation and convolution, (ii) Fourier and discrete Fourier transformation, and (iii

  8. Adaptive Noise Cancellation to Suppress Electrocardiography Artifacts During Real-time Interventional MRI

    PubMed Central

    Wu, Vincent; Barbash, Israel M.; Ratnayaka, Kanishka; Saikus, Christina E.; Sonmez, Merdim; Kocaturk, Ozgur; Lederman, Robert J.; Faranesh, Anthony Z.

    2011-01-01

    PURPOSE To develop a system for artifact suppression in electrocardiogram (ECG) recordings obtained during interventional real-time magnetic resonance imaging. MATERIALS AND METHODS We characterized ECG artifacts due to radiofrequency pulses and gradient switching during MRI in terms of frequency content. A combination of analog filters and digital least mean squares adaptive filters were used to filter the ECG during in vivo experiments and the results were compared with those obtained with simple low-pass filtering. The system performance was evaluated in terms of artifact suppression and ability to identify arrhythmias during real-time MRI. RESULTS Analog filters were able to suppress artifacts from high-frequency radiofrequency pulses and gradient switching. Remaining pulse artifacts caused by intermittent preparation sequences or spoiler gradients required adaptive filtering because their bandwidth overlapped with that of the ECG. Using analog and adaptive filtering, a mean improvement of 38dB (n=11, peak QRS signal to pulse artifact noise) was achieved. This filtering system was successful in removing pulse artifacts which obscured arrhythmias such as premature ventricular complexes and complete atrioventricular block. CONCLUSION We have developed an online ECG monitoring system employing digital adaptive filters which enables the identification of cardiac arrhythmias during real-time MRI-guided interventions. PMID:21509878

  9. Adaptive Kalman filtering for real-time mapping of the visual field.

    PubMed

    Ward, B Douglas; Janik, John; Mazaheri, Yousef; Ma, Yan; DeYoe, Edgar A

    2012-02-15

    This paper demonstrates the feasibility of real-time mapping of the visual field for clinical applications. Specifically, three aspects of this problem were considered: (1) experimental design, (2) statistical analysis, and (3) display of results. Proper experimental design is essential to achieving a successful outcome, particularly for real-time applications. A random-block experimental design was shown to have less sensitivity to measurement noise, as well as greater robustness to error in modeling of the hemodynamic impulse response function (IRF) and greater flexibility than common alternatives. In addition, random encoding of the visual field allows for the detection of voxels that are responsive to multiple, not necessarily contiguous, regions of the visual field. Due to its recursive nature, the Kalman filter is ideally suited for real-time statistical analysis of visual field mapping data. An important feature of the Kalman filter is that it can be used for nonstationary time series analysis. The capability of the Kalman filter to adapt, in real time, to abrupt changes in the baseline arising from subject motion inside the scanner and other external system disturbances is important for the success of clinical applications. The clinician needs real-time information to evaluate the success or failure of the imaging run and to decide whether to extend, modify, or terminate the run. Accordingly, the analytical software provides real-time displays of (1) brain activation maps for each stimulus segment, (2) voxel-wise spatial tuning profiles, (3) time plots of the variability of response parameters, and (4) time plots of activated volume.

  10. Real-time phase measurement of optical vortices based on pixelated micropolarizer array.

    PubMed

    Zhang, Zhigang; Dong, Fengliang; Qian, Kemao; Zhang, Qingchuan; Chu, Weiguo; Zhang, Yuntian; Ma, Xuan; Wu, Xiaoping

    2015-08-10

    The special spiral phase structure of an optical vortex leads to an intriguing study in modern singular optics. This paper proposes a real-time phase measurement method of vortex beam based on pixelated micropolarizer array (PMA). Four phase-shifting fringe images can be obtained from a single interference image, thus the vortex beam phase can be obtained in real-time. The proposed method can achieve full-field phase measurement of the vortex beam with the advantages of lower computation and vibration resistance. In the experiments, the typical phases of vortex with different topological charges are loaded on a spatial light modulator (SLM) to generate diffraction vortex beam, and the phase distribution of vortex beam is obtained in real-time, which confirm the robustness of this method. This method is of great significance in promoting the study of optical vortices.

  11. Miniature real time PCR on chip with multi-channel fiber optical fluorescence detection module.

    PubMed

    Xiang, Q; Xu, B; Li, D

    2007-08-01

    This paper presents the design and implementation of a miniature real time PCR system consisting of a disposable reactor chip, a miniature thermal cycler, and a multi-channel fiber optical fluorescence excitation/detection module. The disposable PCR chip is fabricated by using soft photolithography by PDMS (Polydimethylsiloxane) and glass. The miniature thermal cycler has a thin film heater for heating and a fan for rapid cooling. The fiber optical detection module consists of laser, filter cube, photo-detector and 1x4 fiber optical switch. It is capable of four-well real time PCR analysis. Real-time PCR detection of E. coli stx1 has been demonstrated successfully with this system.

  12. Verification and Validation Methodology of Real-Time Adaptive Neural Networks for Aerospace Applications

    NASA Technical Reports Server (NTRS)

    Gupta, Pramod; Loparo, Kenneth; Mackall, Dale; Schumann, Johann; Soares, Fola

    2004-01-01

    Recent research has shown that adaptive neural based control systems are very effective in restoring stability and control of an aircraft in the presence of damage or failures. The application of an adaptive neural network with a flight critical control system requires a thorough and proven process to ensure safe and proper flight operation. Unique testing tools have been developed as part of a process to perform verification and validation (V&V) of real time adaptive neural networks used in recent adaptive flight control system, to evaluate the performance of the on line trained neural networks. The tools will help in certification from FAA and will help in the successful deployment of neural network based adaptive controllers in safety-critical applications. The process to perform verification and validation is evaluated against a typical neural adaptive controller and the results are discussed.

  13. Real Time Updating Genetic Network Programming for Adapting to the Change of Stock Prices

    NASA Astrophysics Data System (ADS)

    Chen, Yan; Mabu, Shingo; Shimada, Kaoru; Hirasawa, Kotaro

    The key in stock trading model is to take the right actions for trading at the right time, primarily based on the accurate forecast of future stock trends. Since an effective trading with given information of stock prices needs an intelligent strategy for the decision making, we applied Genetic Network Programming (GNP) to creating a stock trading model. In this paper, we propose a new method called Real Time Updating Genetic Network Programming (RTU-GNP) for adapting to the change of stock prices. There are three important points in this paper: First, the RTU-GNP method makes a stock trading decision considering both the recommendable information of technical indices and the candlestick charts according to the real time stock prices. Second, we combine RTU-GNP with a Sarsa learning algorithm to create the programs efficiently. Also, sub-nodes are introduced in each judgment and processing node to determine appropriate actions (buying/selling) and to select appropriate stock price information depending on the situation. Third, a Real Time Updating system has been firstly introduced in our paper considering the change of the trend of stock prices. The experimental results on the Japanese stock market show that the trading model with the proposed RTU-GNP method outperforms other models without real time updating. We also compared the experimental results using the proposed method with Buy&Hold method to confirm its effectiveness, and it is clarified that the proposed trading model can obtain much higher profits than Buy&Hold method.

  14. Adaptation of adaptive optics systems.

    NASA Astrophysics Data System (ADS)

    Xin, Yu; Zhao, Dazun; Li, Chen

    1997-10-01

    In the paper, a concept of an adaptation of adaptive optical system (AAOS) is proposed. The AAOS has certain real time optimization ability against the variation of the brightness of detected objects m, atmospheric coherence length rO and atmospheric time constant τ by means of changing subaperture number and diameter, dynamic range, and system's temporal response. The necessity of AAOS using a Hartmann-Shack wavefront sensor and some technical approaches are discussed. Scheme and simulation of an AAOS with variable subaperture ability by use of both hardware and software are presented as an example of the system.

  15. Optimized quantum sensing with a single electron spin using real-time adaptive measurements

    NASA Astrophysics Data System (ADS)

    Bonato, C.; Blok, M. S.; Dinani, H. T.; Berry, D. W.; Markham, M. L.; Twitchen, D. J.; Hanson, R.

    2016-03-01

    Quantum sensors based on single solid-state spins promise a unique combination of sensitivity and spatial resolution. The key challenge in sensing is to achieve minimum estimation uncertainty within a given time and with high dynamic range. Adaptive strategies have been proposed to achieve optimal performance, but their implementation in solid-state systems has been hindered by the demanding experimental requirements. Here, we realize adaptive d.c. sensing by combining single-shot readout of an electron spin in diamond with fast feedback. By adapting the spin readout basis in real time based on previous outcomes, we demonstrate a sensitivity in Ramsey interferometry surpassing the standard measurement limit. Furthermore, we find by simulations and experiments that adaptive protocols offer a distinctive advantage over the best known non-adaptive protocols when overhead and limited estimation time are taken into account. Using an optimized adaptive protocol we achieve a magnetic field sensitivity of 6.1 ± 1.7 nT Hz-1/2 over a wide range of 1.78 mT. These results open up a new class of experiments for solid-state sensors in which real-time knowledge of the measurement history is exploited to obtain optimal performance.

  16. Real-time optical subtraction of photographic imagery for difference detection.

    PubMed

    Ebersole, J F; Wyant, J C

    1976-04-01

    Interferometric techniques described in this paper permit real-time optical image subtraction of two input photograph transparencies without the necessity of intermediate processing steps (e.g., holograms or contact-print transparencies). These interferometric techniques allow the use of a white-light source as well as an extended light source, small input-collimator optics, and optical components with minimal requirements on wavefront quality. Experimental results with NASA LANDSAT (formerly ERTS) photographs are presented.

  17. Real-Time Robust Adaptive Modeling and Scheduling for an Electronic Commerce Server

    NASA Astrophysics Data System (ADS)

    Du, Bing; Ruan, Chun

    With the increasing importance and pervasiveness of Internet services, it is becoming a challenge for the proliferation of electronic commerce services to provide performance guarantees under extreme overload. This paper describes a real-time optimization modeling and scheduling approach for performance guarantee of electronic commerce servers. We show that an electronic commerce server may be simulated as a multi-tank system. A robust adaptive server model is subject to unknown additive load disturbances and uncertain model matching. Overload control techniques are based on adaptive admission control to achieve timing guarantees. We evaluate the performance of the model using a complex simulation that is subjected to varying model parameters and massive overload.

  18. Real-time artificial intelligence issues in the development of the adaptive tactical navigator

    NASA Technical Reports Server (NTRS)

    Green, Peter E.; Glasson, Douglas P.; Pomarede, Jean-Michel L.; Acharya, Narayan A.

    1987-01-01

    Adaptive Tactical Navigation (ATN) is a laboratory prototype of a knowledge based system to provide navigation system management and decision aiding in the next generation of tactical aircraft. ATN's purpose is to manage a set of multimode navigation equipment, dynamically selecting the best equipment to use in accordance with mission goals and phase, threat environment, equipment malfunction status, and battle damage. ATN encompasses functions as diverse as sensor data interpretation, diagnosis, and planning. Real time issues that were identified in ATN and the approaches used to address them are addressed. Functional requirements and a global architecture for the ATN system are described. Decision making with time constraints are discussed. Two subproblems are identified; making decisions with incomplete information and with limited resources. Approaches used in ATN to address real time performance are described and simulation results are discussed.

  19. Enhancing the Quality of Service for Real Time Traffic over Optical Burst Switching (OBS) Networks with Ensuring the Fairness for Other Traffics

    PubMed Central

    Al-Shargabi, Mohammed A.; Ismail, Abdulsamad S.

    2016-01-01

    Optical burst switching (OBS) networks have been attracting much consideration as a promising approach to build the next generation optical Internet. A solution for enhancing the Quality of Service (QoS) for high priority real time traffic over OBS with the fairness among the traffic types is absent in current OBS’ QoS schemes. In this paper we present a novel Real Time Quality of Service with Fairness Ratio (RT-QoSFR) scheme that can adapt the burst assembly parameters according to the traffic QoS needs in order to enhance the real time traffic QoS requirements and to ensure the fairness for other traffic. The results show that RT-QoSFR scheme is able to fulfill the real time traffic requirements (end to end delay, and loss rate) ensuring the fairness for other traffics under various conditions such as the type of real time traffic and traffic load. RT-QoSFR can guarantee that the delay of the real time traffic packets does not exceed the maximum packets transfer delay value. Furthermore, it can reduce the real time traffic packets loss, at the same time guarantee the fairness for non real time traffic packets by determining the ratio of real time traffic inside the burst to be 50–60%, 30–40%, and 10–20% for high, normal, and low traffic loads respectively. PMID:27583557

  20. Enhancing the Quality of Service for Real Time Traffic over Optical Burst Switching (OBS) Networks with Ensuring the Fairness for Other Traffics.

    PubMed

    Al-Shargabi, Mohammed A; Shaikh, Asadullah; Ismail, Abdulsamad S

    2016-01-01

    Optical burst switching (OBS) networks have been attracting much consideration as a promising approach to build the next generation optical Internet. A solution for enhancing the Quality of Service (QoS) for high priority real time traffic over OBS with the fairness among the traffic types is absent in current OBS' QoS schemes. In this paper we present a novel Real Time Quality of Service with Fairness Ratio (RT-QoSFR) scheme that can adapt the burst assembly parameters according to the traffic QoS needs in order to enhance the real time traffic QoS requirements and to ensure the fairness for other traffic. The results show that RT-QoSFR scheme is able to fulfill the real time traffic requirements (end to end delay, and loss rate) ensuring the fairness for other traffics under various conditions such as the type of real time traffic and traffic load. RT-QoSFR can guarantee that the delay of the real time traffic packets does not exceed the maximum packets transfer delay value. Furthermore, it can reduce the real time traffic packets loss, at the same time guarantee the fairness for non real time traffic packets by determining the ratio of real time traffic inside the burst to be 50-60%, 30-40%, and 10-20% for high, normal, and low traffic loads respectively.

  1. Acousto-Optic Interaction in Surface Acoustic Waves and Its Application to Real Time Signal Processing.

    DTIC Science & Technology

    1977-12-30

    ACOUSTO - OPTIC INTERACTION IN SURFACE ACOUSTIC WAVES AND ITS APP--ETC(U) DEC 77 0 SCHUMER, P DAS NOOOIJ -75-C-0772 NCLASSIFIED MA-ONR-30 Nt.EE E’h...CHART NAT*NAL BUREAU OF STANDARDS 1-63- ACOUSTO - OPTIC INTERACTION IN SURFACE ACOUSTIC WAVES AND ITS APPLICATION TO REAL TIME SIGNAL PROCESSING By 00 D... Acousto - optics , Integrated optics, Optical Signal Processing. 20. AbSKTRACT (Continue an reverse side it neceary and idewnt& by block mum ber) The

  2. A novel algorithm for real-time adaptive signal detection and identification

    SciTech Connect

    Sleefe, G.E.; Ladd, M.D.; Gallegos, D.E.; Sicking, C.W.; Erteza, I.A.

    1998-04-01

    This paper describes a novel digital signal processing algorithm for adaptively detecting and identifying signals buried in noise. The algorithm continually computes and updates the long-term statistics and spectral characteristics of the background noise. Using this noise model, a set of adaptive thresholds and matched digital filters are implemented to enhance and detect signals that are buried in the noise. The algorithm furthermore automatically suppresses coherent noise sources and adapts to time-varying signal conditions. Signal detection is performed in both the time-domain and the frequency-domain, thereby permitting the detection of both broad-band transients and narrow-band signals. The detection algorithm also provides for the computation of important signal features such as amplitude, timing, and phase information. Signal identification is achieved through a combination of frequency-domain template matching and spectral peak picking. The algorithm described herein is well suited for real-time implementation on digital signal processing hardware. This paper presents the theory of the adaptive algorithm, provides an algorithmic block diagram, and demonstrate its implementation and performance with real-world data. The computational efficiency of the algorithm is demonstrated through benchmarks on specific DSP hardware. The applications for this algorithm, which range from vibration analysis to real-time image processing, are also discussed.

  3. An optical real-time 3D measurement for analysis of facial shape and movement

    NASA Astrophysics Data System (ADS)

    Zhang, Qican; Su, Xianyu; Chen, Wenjing; Cao, Yiping; Xiang, Liqun

    2003-12-01

    Optical non-contact 3-D shape measurement provides a novel and useful tool for analysis of facial shape and movement in presurgical and postsurgical regular check. In this article we present a system, which allows a precise 3-D visualization of the patient's facial before and after craniofacial surgery. We discussed, in this paper, the real time 3-D image capture, processing and the 3-D phase unwrapping method to recover complex shape deformation when the movement of the mouth. The result of real-time measurement for facial shape and movement will be helpful for the more ideal effect in plastic surgery.

  4. Real-Time Optical Monitoring of Pt Catalyst Under the Potentiodynamic Conditions

    NASA Astrophysics Data System (ADS)

    Song, Hyeon Don; Lee, Minzae; Kim, Gil-Pyo; Choi, Inhee; Yi, Jongheop

    2016-12-01

    In situ monitoring of electrode materials reveals detailed physicochemical transition in electrochemical device. The key challenge is to explore the localized features of electrode surfaces, since the performance of an electrochemical device is determined by the summation of local architecture of the electrode material. Adaptive in situ techniques have been developed for numerous investigations; however, they require restricted measurement environments and provide limited information, which has impeded their widespread application. In this study, we realised an optics-based electrochemical in situ monitoring system by combining a dark-field micro/spectroscopy with an electrochemical workstation to investigate the physicochemical behaviours of Pt catalyst. We found that the localized plasmonic trait of a Pt-decorated Au nanoparticle as a model system varied in terms of its intensity and wavelength during the iterations of a cyclic voltammetry test. Furthermore, we show that morphological and compositional changes of the Pt catalyst can be traced in real time using changes in quantified plasmonic characteristics, which is a distinct advantage over the conventional electrochemistry-based in situ monitoring systems. These results indicate the substantial promise of online operando observation in a wide range of electrical energy conversion systems and electrochemical sensing areas.

  5. Real-Time Optical Monitoring of Pt Catalyst Under the Potentiodynamic Conditions

    PubMed Central

    Song, Hyeon Don; Lee, Minzae; Kim, Gil-Pyo; Choi, Inhee; Yi, Jongheop

    2016-01-01

    In situ monitoring of electrode materials reveals detailed physicochemical transition in electrochemical device. The key challenge is to explore the localized features of electrode surfaces, since the performance of an electrochemical device is determined by the summation of local architecture of the electrode material. Adaptive in situ techniques have been developed for numerous investigations; however, they require restricted measurement environments and provide limited information, which has impeded their widespread application. In this study, we realised an optics-based electrochemical in situ monitoring system by combining a dark-field micro/spectroscopy with an electrochemical workstation to investigate the physicochemical behaviours of Pt catalyst. We found that the localized plasmonic trait of a Pt-decorated Au nanoparticle as a model system varied in terms of its intensity and wavelength during the iterations of a cyclic voltammetry test. Furthermore, we show that morphological and compositional changes of the Pt catalyst can be traced in real time using changes in quantified plasmonic characteristics, which is a distinct advantage over the conventional electrochemistry-based in situ monitoring systems. These results indicate the substantial promise of online operando observation in a wide range of electrical energy conversion systems and electrochemical sensing areas. PMID:27934945

  6. Miniaturized and Wireless Optical Neurotransmitter Sensor for Real-Time Monitoring of Dopamine in the Brain

    PubMed Central

    Kim, Min H.; Yoon, Hargsoon; Choi, Sang H.; Zhao, Fei; Kim, Jongsung; Song, Kyo D.; Lee, Uhn

    2016-01-01

    Real-time monitoring of extracellular neurotransmitter concentration offers great benefits for diagnosis and treatment of neurological disorders and diseases. This paper presents the study design and results of a miniaturized and wireless optical neurotransmitter sensor (MWONS) for real-time monitoring of brain dopamine concentration. MWONS is based on fluorescent sensing principles and comprises a microspectrometer unit, a microcontroller for data acquisition, and a Bluetooth wireless network for real-time monitoring. MWONS has a custom-designed application software that controls the operation parameters for excitation light sources, data acquisition, and signal processing. MWONS successfully demonstrated a measurement capability with a limit of detection down to a 100 nanomole dopamine concentration, and high selectivity to ascorbic acid (90:1) and uric acid (36:1). PMID:27834927

  7. Real-time In-Flight Strain and Deflection Monitoring with Fiber Optic Sensors

    NASA Technical Reports Server (NTRS)

    Richards, Lance; Parker, Allen R.; Ko, William L.; Piazza, Anthony

    2008-01-01

    This viewgraph presentation reviews Dryden's efforts to develop in-flight monitoring based on Fiber Optics. One of the motivating factors for this development was the breakup of the Helios aircraft. On Ikhana the use of fiber optics for wing shape sensing is being developed. They are being used to flight validate fiber optic sensor measurements and real-time wing shape sensing predictions on NASA's Ikhana vehicle; validate fiber optic mathematical models and design tools; Assess technical viability and, if applicable, develop methodology and approach to incorporate wing shape measurements within the vehicle flight control system, and develop and flight validate advanced approaches to perform active wing shape control.

  8. Real-Time Adaptive Control Allocation Applied to a High Performance Aircraft

    NASA Technical Reports Server (NTRS)

    Davidson, John B.; Lallman, Frederick J.; Bundick, W. Thomas

    2001-01-01

    Abstract This paper presents the development and application of one approach to the control of aircraft with large numbers of control effectors. This approach, referred to as real-time adaptive control allocation, combines a nonlinear method for control allocation with actuator failure detection and isolation. The control allocator maps moment (or angular acceleration) commands into physical control effector commands as functions of individual control effectiveness and availability. The actuator failure detection and isolation algorithm is a model-based approach that uses models of the actuators to predict actuator behavior and an adaptive decision threshold to achieve acceptable false alarm/missed detection rates. This integrated approach provides control reconfiguration when an aircraft is subjected to actuator failure, thereby improving maneuverability and survivability of the degraded aircraft. This method is demonstrated on a next generation military aircraft Lockheed-Martin Innovative Control Effector) simulation that has been modified to include a novel nonlinear fluid flow control control effector based on passive porosity. Desktop and real-time piloted simulation results demonstrate the performance of this integrated adaptive control allocation approach.

  9. Estimating Model Parameters of Adaptive Software Systems in Real-Time

    NASA Astrophysics Data System (ADS)

    Kumar, Dinesh; Tantawi, Asser; Zhang, Li

    Adaptive software systems have the ability to adapt to changes in workload and execution environment. In order to perform resource management through model based control in such systems, an accurate mechanism for estimating the software system's model parameters is required. This paper deals with real-time estimation of a performance model for adaptive software systems that process multiple classes of transactional workload. First, insights in to the static performance model estimation problem are provided. Then an Extended Kalman Filter (EKF) design is combined with an open queueing network model to dynamically estimate the model parameters in real-time. Specific problems that are encountered in the case of multiple classes of workload are analyzed. These problems arise mainly due to the under-deterministic nature of the estimation problem. This motivates us to propose a modified design of the filter. Insights for choosing tuning parameters of the modified design, i.e., number of constraints and sampling intervals are provided. The modified filter design is shown to effectively tackle problems with multiple classes of workload through experiments.

  10. Real-time dual-microphone noise classification for environment-adaptive pipelines of cochlear implants.

    PubMed

    Mirzahasanloo, Taher; Kehtarnavaz, Nasser

    2013-01-01

    This paper presents an improved noise classification in environment-adaptive speech processing pipelines of cochlear implants. This improvement is achieved by using a dual-microphone and by using a computationally efficient feature-level combination approach to achieve real-time operation. A new measure named Suppression Advantage is also defined in order to quantify the noise suppression improvement of an entire pipeline due to noise classification. The noise classification and suppression improvement results are presented for four commonly encountered noise environments.

  11. A robust adaptive denoising framework for real-time artifact removal in scalp EEG measurements

    NASA Astrophysics Data System (ADS)

    Kilicarslan, Atilla; Grossman, Robert G.; Contreras-Vidal, Jose Luis

    2016-04-01

    Objective. Non-invasive measurement of human neural activity based on the scalp electroencephalogram (EEG) allows for the development of biomedical devices that interface with the nervous system for scientific, diagnostic, therapeutic, or restorative purposes. However, EEG recordings are often considered as prone to physiological and non-physiological artifacts of different types and frequency characteristics. Among them, ocular artifacts and signal drifts represent major sources of EEG contamination, particularly in real-time closed-loop brain-machine interface (BMI) applications, which require effective handling of these artifacts across sessions and in natural settings. Approach. We extend the usage of a robust adaptive noise cancelling (ANC) scheme ({H}∞ filtering) for removal of eye blinks, eye motions, amplitude drifts and recording biases simultaneously. We also characterize the volume conduction, by estimating the signal propagation levels across all EEG scalp recording areas due to ocular artifact generators. We find that the amplitude and spatial distribution of ocular artifacts vary greatly depending on the electrode location. Therefore, fixed filtering parameters for all recording areas would naturally hinder the true overall performance of an ANC scheme for artifact removal. We treat each electrode as a separate sub-system to be filtered, and without the loss of generality, they are assumed to be uncorrelated and uncoupled. Main results. Our results show over 95-99.9% correlation between the raw and processed signals at non-ocular artifact regions, and depending on the contamination profile, 40-70% correlation when ocular artifacts are dominant. We also compare our results with the offline independent component analysis and artifact subspace reconstruction methods, and show that some local quantities are handled better by our sample-adaptive real-time framework. Decoding performance is also compared with multi-day experimental data from 2 subjects

  12. Robust adaptive extended Kalman filtering for real time MR-thermometry guided HIFU interventions.

    PubMed

    Roujol, Sébastien; de Senneville, Baudouin Denis; Hey, Silke; Moonen, Chrit; Ries, Mario

    2012-03-01

    Real time magnetic resonance (MR) thermometry is gaining clinical importance for monitoring and guiding high intensity focused ultrasound (HIFU) ablations of tumorous tissue. The temperature information can be employed to adjust the position and the power of the HIFU system in real time and to determine the therapy endpoint. The requirement to resolve both physiological motion of mobile organs and the rapid temperature variations induced by state-of-the-art high-power HIFU systems require fast MRI-acquisition schemes, which are generally hampered by low signal-to-noise ratios (SNRs). This directly limits the precision of real time MR-thermometry and thus in many cases the feasibility of sophisticated control algorithms. To overcome these limitations, temporal filtering of the temperature has been suggested in the past, which has generally an adverse impact on the accuracy and latency of the filtered data. Here, we propose a novel filter that aims to improve the precision of MR-thermometry while monitoring and adapting its impact on the accuracy. For this, an adaptive extended Kalman filter using a model describing the heat transfer for acoustic heating in biological tissues was employed together with an additional outlier rejection to address the problem of sparse artifacted temperature points. The filter was compared to an efficient matched FIR filter and outperformed the latter in all tested cases. The filter was first evaluated on simulated data and provided in the worst case (with an approximate configuration of the model) a substantial improvement of the accuracy by a factor 3 and 15 during heat up and cool down periods, respectively. The robustness of the filter was then evaluated during HIFU experiments on a phantom and in vivo in porcine kidney. The presence of strong temperature artifacts did not affect the thermal dose measurement using our filter whereas a high measurement variation of 70% was observed with the FIR filter.

  13. Searching for optical transients in real-time : the RAPTOR experiment /.

    SciTech Connect

    Vestrand, W. T.; Borozdin, K. N.; Brumby, Steven P.; Casperson, D. E.; Fenimore, Edward E.; Galassi, M. C.; Gisler, Galen R.; McGowan, K. E.; Perkins, S. J.; Priedhorsky, W. C.; Starr, D. L.; White, R. A.; Wozniak, Przemyslaw; Wren, J.

    2002-01-01

    A rich, but relatively unexplored, region in optical astronomy is the study of transients with durations of less than a day. We describe a wide-field optical monitoring system, RAPTOR, which is designed to identify and make follow-up observations of optical transients in real-time. The system is composed of an array of telescopes that continuously monitor about 1500 square degrees of the sky for transients down to about 12' magnitude in 60 seconds and a central fovea telescope that can reach 16{approx}m' agnitude in 60 seconds. Coupled to the telescope array is a real-time data analysis pipeline that is designed to identify transients on timescales of seconds. In a manner analogous to human vision, the entire array is mounted on a rapidly slewing robotic mount so that the fovea of the array can be rapidly directed at transients identified by the wide-field system. The goal of the project is to develop a ground-based optical system that can reliably identify transients in real-time and ultimately generate alerts with source locations to enable follow-up observations wilh other, larger, telescopes.

  14. Data rate management and real time operation: recursive adaptive frame integration of limited data

    NASA Astrophysics Data System (ADS)

    Rafailov, Michael K.

    2006-08-01

    Recursive Limited Frame Integration was proposed as a way to improve frame integration performance and mitigate issues related to high data rate needed to support conventional frame integration. The technique uses two thresholds -one tuned for optimum probability of detection, the other to manage required false alarm rate, and places integration process between those thresholds. This configuration allows a non-linear integration process that, along with Signal-to-Noise Ratio (SNR) gain, provides system designers more capability where cost, weight, or power considerations limit system data rate, processing, or memory capability. However, Recursive Frame Integration Limited may have performance issues when single-frame SNR is really low. Recursive Adaptive Limited Frame Integration was proposed as a means to improve limited integration performance with really low single-frame SNR. It combines the benefits of nonlinear recursive limited frame integration and adaptive thresholds with a kind of conventional frame integration. Adding the third threshold may help in managing real time operations. In the paper the Recursive Frame Integration is presented in form of multiple parallel recursive integration. Such an approach can help not only in data rate management but in mitigation of low single frame SNR issue for Recursive Integration as well as in real time operations with frame integration.

  15. Real-Time Adaptive EEG Source Separation Using Online Recursive Independent Component Analysis.

    PubMed

    Hsu, Sheng-Hsiou; Mullen, Tim R; Jung, Tzyy-Ping; Cauwenberghs, Gert

    2016-03-01

    Independent component analysis (ICA) has been widely applied to electroencephalographic (EEG) biosignal processing and brain-computer interfaces. The practical use of ICA, however, is limited by its computational complexity, data requirements for convergence, and assumption of data stationarity, especially for high-density data. Here we study and validate an optimized online recursive ICA algorithm (ORICA) with online recursive least squares (RLS) whitening for blind source separation of high-density EEG data, which offers instantaneous incremental convergence upon presentation of new data. Empirical results of this study demonstrate the algorithm's: 1) suitability for accurate and efficient source identification in high-density (64-channel) realistically-simulated EEG data; 2) capability to detect and adapt to nonstationarity in 64-ch simulated EEG data; and 3) utility for rapidly extracting principal brain and artifact sources in real 61-channel EEG data recorded by a dry and wearable EEG system in a cognitive experiment. ORICA was implemented as functions in BCILAB and EEGLAB and was integrated in an open-source Real-time EEG Source-mapping Toolbox (REST), supporting applications in ICA-based online artifact rejection, feature extraction for real-time biosignal monitoring in clinical environments, and adaptable classifications in brain-computer interfaces.

  16. Real-time Adaptive EEG Source Separation using Online Recursive Independent Component Analysis

    PubMed Central

    Hsu, Sheng-Hsiou; Mullen, Tim; Jung, Tzyy-Ping; Cauwenberghs, Gert

    2016-01-01

    Independent Component Analysis (ICA) has been widely applied to electroencephalographic (EEG) biosignal processing and brain-computer interfaces. The practical use of ICA, however, is limited by its computational complexity, data requirements for convergence, and assumption of data stationarity, especially for high-density data. Here we study and validate an optimized online recursive ICA algorithm (ORICA) with online recursive least squares (RLS) whitening for blind source separation of high-density EEG data, which offers instantaneous incremental convergence upon presentation of new data. Empirical results of this study demonstrate the algorithm's: (a) suitability for accurate and efficient source identification in high-density (64-channel) realistically-simulated EEG data; (b) capability to detect and adapt to non-stationarity in 64-ch simulated EEG data; and (c) utility for rapidly extracting principal brain and artifact sources in real 61-channel EEG data recorded by a dry and wearable EEG system in a cognitive experiment. ORICA was implemented as functions in BCILAB and EEGLAB and was integrated in an open-source Real-time EEG Source-mapping Toolbox (REST), supporting applications in ICA-based online artifact rejection, feature extraction for real-time biosignal monitoring in clinical environments, and adaptable classifications in brain-computer interfaces. PMID:26685257

  17. Real-time task recognition in cataract surgery videos using adaptive spatiotemporal polynomials.

    PubMed

    Quellec, Gwénolé; Lamard, Mathieu; Cochener, Béatrice; Cazuguel, Guy

    2015-04-01

    This paper introduces a new algorithm for recognizing surgical tasks in real-time in a video stream. The goal is to communicate information to the surgeon in due time during a video-monitored surgery. The proposed algorithm is applied to cataract surgery, which is the most common eye surgery. To compensate for eye motion and zoom level variations, cataract surgery videos are first normalized. Then, the motion content of short video subsequences is characterized with spatiotemporal polynomials: a multiscale motion characterization based on adaptive spatiotemporal polynomials is presented. The proposed solution is particularly suited to characterize deformable moving objects with fuzzy borders, which are typically found in surgical videos. Given a target surgical task, the system is trained to identify which spatiotemporal polynomials are usually extracted from videos when and only when this task is being performed. These key spatiotemporal polynomials are then searched in new videos to recognize the target surgical task. For improved performances, the system jointly adapts the spatiotemporal polynomial basis and identifies the key spatiotemporal polynomials using the multiple-instance learning paradigm. The proposed system runs in real-time and outperforms the previous solution from our group, both for surgical task recognition ( Az = 0.851 on average, as opposed to Az = 0.794 previously) and for the joint segmentation and recognition of surgical tasks ( Az = 0.856 on average, as opposed to Az = 0.832 previously).

  18. Real-time Graphics Processing Unit Based Fourier Domain Optical Coherence Tomography and Surgical Applications

    NASA Astrophysics Data System (ADS)

    Zhang, Kang

    2011-12-01

    In this dissertation, real-time Fourier domain optical coherence tomography (FD-OCT) capable of multi-dimensional micrometer-resolution imaging targeted specifically for microsurgical intervention applications was developed and studied. As a part of this work several ultra-high speed real-time FD-OCT imaging and sensing systems were proposed and developed. A real-time 4D (3D+time) OCT system platform using the graphics processing unit (GPU) to accelerate OCT signal processing, the imaging reconstruction, visualization, and volume rendering was developed. Several GPU based algorithms such as non-uniform fast Fourier transform (NUFFT), numerical dispersion compensation, and multi-GPU implementation were developed to improve the impulse response, SNR roll-off and stability of the system. Full-range complex-conjugate-free FD-OCT was also implemented on the GPU architecture to achieve doubled image range and improved SNR. These technologies overcome the imaging reconstruction and visualization bottlenecks widely exist in current ultra-high speed FD-OCT systems and open the way to interventional OCT imaging for applications in guided microsurgery. A hand-held common-path optical coherence tomography (CP-OCT) distance-sensor based microsurgical tool was developed and validated. Through real-time signal processing, edge detection and feed-back control, the tool was shown to be capable of track target surface and compensate motion. The micro-incision test using a phantom was performed using a CP-OCT-sensor integrated hand-held tool, which showed an incision error less than +/-5 microns, comparing to >100 microns error by free-hand incision. The CP-OCT distance sensor has also been utilized to enhance the accuracy and safety of optical nerve stimulation. Finally, several experiments were conducted to validate the system for surgical applications. One of them involved 4D OCT guided micro-manipulation using a phantom. Multiple volume renderings of one 3D data set were

  19. Real-time inversion of polarization gate frequency-resolved optical gating spectrograms.

    PubMed

    Kane, Daniel J; Weston, Jeremy; Chu, Kai-Chien J

    2003-02-20

    Frequency-resolved optical gating (FROG) is a technique used to measure the intensity and phase of ultrashort laser pulses through the optical construction of a spectrogram of the pulse. To obtain quantitative information about the pulse from its spectrogram, an iterative two-dimensional phase retrieval algorithm must be used. Current algorithms are quite robust but retrieval of all the pulse information can be slow. Previous real-time FROG trace inversion work focused on second-harmonic-generation FROG, which has an ambiguity in the direction of time, and required digital signal processors (DSPs). We develop a simplified real-time FROG device based on a single-shot geometry that no longer requires DSPs. We use it and apply the principal component generalized projections algorithm to invert polarization gate FROG traces at rates as high as 20 Hz.

  20. Real-time detection of generic objects using objectness estimation and locally adaptive regression kernels matching

    NASA Astrophysics Data System (ADS)

    Zheng, Zhihui; Gao, Lei; Xiao, Liping; Zhou, Bin; Gao, Shibo

    2015-12-01

    Our purpose is to develop a detection algorithm capable of searching for generic interest objects in real time without large training sets and long-time training stages. Instead of the classical sliding window object detection paradigm, we employ an objectness measure to produce a small set of candidate windows efficiently using Binarized Normed Gradients and a Laplacian of Gaussian-like filter. We then extract Locally Adaptive Regression Kernels (LARKs) as descriptors both from a model image and the candidate windows which measure the likeness of a pixel to its surroundings. Using a matrix cosine similarity measure, the algorithm yields a scalar resemblance map, indicating the likelihood of similarity between the model and the candidate windows. By employing nonparametric significance tests and non-maxima suppression, we detect the presence of objects similar to the given model. Experiments show that the proposed detection paradigm can automatically detect the presence, the number, as well as location of similar objects to the given model. The high quality and efficiency of our method make it suitable for real time multi-category object detection applications.

  1. Nanostructured biochip for label-free and real-time optical detection of polymerase chain reaction.

    PubMed

    Hiep, Ha Minh; Kerman, Kagan; Endo, Tatsuro; Saito, Masato; Tamiya, Eiichi

    2010-02-19

    In this report, Au-coated nanostructured biochip with functionalized thiolated primers on its surface is developed for label-free and real-time optical detection of polymerase chain reaction (PCR). A PCR chamber of 150 microm in thickness containing Au-coated nanostructured substrate in the bottom layer was bordered with SU-8 100 walls. After immobilization of 5'-thiolated primers on the surface, simultaneous DNA amplification and detection were performed without any labeled molecules via the relative reflected intensity (RRI) of Au-coated nanostructured substrate. When human genomic DNA at several concentrations of 0.2, 0.5 and 1 ng microL(-1) was included in the initial DNA samples, the increases in the RRI peak values were clearly observed with the increasing PCR cycle numbers. We found that the starting point of the optical signal, which was divergent from the background in our PCR biochip, was around 3-4 cycles, much lower than that of the fluorescent real-time PCR analysis (around 23-25 cycles). Our proposed PCR device using Au-coated nanostructured substrate holds noteworthy promise for rapid, label-free and real-time DNA detection for point-of-care testing (POCT) applications.

  2. Real-time particle tracking at 10,000 fps using optical fiber illumination.

    PubMed

    Otto, Oliver; Czerwinski, Fabian; Gornall, Joanne L; Stober, Gunter; Oddershede, Lene B; Seidel, Ralf; Keyser, Ulrich F

    2010-10-25

    We introduce optical fiber illumination for real-time tracking of optically trapped micrometer-sized particles with microsecond time resolution. Our light source is a high-radiance mercury arc lamp and a 600 μm optical fiber for short-distance illumination of the sample cell. Particle tracking is carried out with a software implemented cross-correlation algorithm following image acquisition from a CMOS camera. Our image data reveals that fiber illumination results in a signal-to-noise ratio usually one order of magnitude higher compared to standard Köhler illumination. We demonstrate position determination of a single optically trapped colloid with up to 10,000 frames per second over hours. We calibrate our optical tweezers and compare the results with quadrant photo diode measurements. Finally, we determine the positional accuracy of our setup to 2 nm by calculating the Allan variance. Our results show that neither illumination nor software algorithms limit the speed of real-time particle tracking with CMOS technology.

  3. Real-time locating and speed measurement of fibre fuse using optical frequency-domain reflectometry

    PubMed Central

    Jiang, Shoulin; Ma, Lin; Fan, Xinyu; Wang, Bin; He, Zuyuan

    2016-01-01

    We propose and experimentally demonstrate real-time locating and speed measurement of fibre fuse by analysing the Doppler shift of reflected light using optical frequency-domain reflectometry (OFDR). Our method can detect the start of a fibre fuse within 200 ms which is equivalent to a propagation distance of about 10 cm in standard single-mode fibre. We successfully measured instantaneous speed of propagating fibre fuses and observed their subtle fluctuation owing to the laser power instability. The resolution achieved for speed measurement in our demonstration is 1 × 10−3 m/s. We studied the fibre fuse propagation speed dependence on the launched power in different fibres. Our method is promising for both real time fibre fuse monitoring and future studies on its propagation and termination. PMID:27146550

  4. Real-time optimal adaptation for planetary geometry and texture: 4-8 tile hierarchies.

    PubMed

    Hwa, Lok M; Duchaineau, Mark A; Joy, Kenneth I

    2005-01-01

    The real-time display of huge geometry and imagery databases involves view-dependent approximations, typically through the use of precomputed hierarchies that are selectively refined at runtime. A classic motivating problem is terrain visualization in which planetary databases involving billions of elevation and color values are displayed on PC graphics hardware at high frame rates. This paper introduces a new diamond data structure for the basic selective-refinement processing, which is a streamlined method of representing the well-known hierarchies of right triangles that have enjoyed much success in real-time, view-dependent terrain display. Regular-grid tiles are proposed as the payload data per diamond for both geometry and texture. The use of 4-8 grid refinement and coarsening schemes allows level-of-detail transitions that are twice as gradual as traditional quadtree-based hierarchies, as well as very high-quality low-pass filtering compared to subsampling-based hierarchies. An out-of-core storage organization is introduced based on Sierpinski indices per diamond, along with a tile preprocessing framework based on fine-to-coarse, same-level, and coarse-to-fine gathering operations. To attain optimal frame-to-frame coherence and processing-order priorities, dual split and merge queues are developed similar to the Realtime Optimally Adapting Meshes (ROAM) Algorithm, as well as an adaptation of the ROAM frustum culling technique. Example applications of lake-detection and procedural terrain generation demonstrate the flexibility of the tile processing framework.

  5. Real-time and static in vivo ophthalmic imaging by spectral optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Wojtkowski, Maciej; Bajraszewski, Tomasz; Targowski, Piotr; Kowalczyk, Andrzej

    2004-07-01

    Fast Spectral Optical Coherence Tomography (SOCT) technique is used to perform cross sectional and three-dimensional ophthalmic images. Static, real-time and 3-D in vivo images of the human cornea, lens, iris, corneo-scleral junction, retinal layers, optic disc and macula lutea are presented. The ophthalmic application of SOCT is promising because this technique ensures fast acquisition with relatively low optical power of incident light. All demonstrated images are obtained with the aid of SOCT instrument, which was constructed in the optical laboratory of medical physics group at Nicolaus Copernicus University (Torun, Poland). What is to our knowledge there are the first good quality (>90dB sensitivity) ophthalmic OCT images obtained by technique, which is different than time domain OCT.

  6. Real-time optical diagnosis of gastric cancer with serosal invasion using multiphoton imaging

    PubMed Central

    Yan, Jun; Zheng, Yu; Zheng, Xiaoling; Liu, Zhangyuanzhu; Liu, Wenju; Chen, Dexin; Dong, Xiaoyu; Li, Kai; Liu, Xiumin; Chen, Gang; Lu, Jianping; Chen, Jianxin; Zhuo, Shuangmu; Li, Guoxin

    2016-01-01

    A real-time optical biopsy, which could determine tissue histopathology, would be of extraordinary benefit to staging laparoscopy for gastric cancer with serosal invasion (T4) that requires downstage treatment. We investigated the feasibility of using multiphoton imaging to perform a real-time optical diagnosis of gastric cancer with or without serosal invasion. First, a pilot study was performed to establish the optical diagnostic features of gastric cancer with or without serosal invasion using multiphoton imaging compared with hematoxylin-eosin staining and Masson’s trichrome staining. Second, a blinded study was performed to compare the diagnostic sensitivity, specificity, and accuracy of multiphoton imaging and endoscopic ultrasonography (EUS) for T4 gastric cancer. In the pilot study, multiphoton imaging revealed collagen loss and degradation and cellular and nuclear pleomorphism in gastric cancer with serosal invasion. The collagen content in gastric cancer with or without serosal invasion was 0.36 ± 0.18 and 0.79 ± 0.16 (p < 0.001), respectively. In the blinded study, the sensitivity, specificity, and accuracy of EUS and multiphoton imaging for T4 gastric cancer were 70% and 90% (p = 0.029), 66.67% and 96.67% (p = 0.003), and 68.33% and 93.33% (p = 0.001), respectively. It is feasible to use multiphoton imaging to make a real-time optical diagnosis of gastric cancer with or without serosal invasion. PMID:27499365

  7. Real-time endoscopic oxygenation imaging using single snapshot of optical properties (SSOP) imaging (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Angelo, Joseph P.; van de Giessen, Martijn; Gioux, Sylvain

    2016-03-01

    With 50% of all interventional procedures in the US being minimally invasive, there is a need for objective tools to help guide surgeons in this challenging environment. Tissue oxygenation is a useful biomarker of tissue viability and suitable for surgical guidance. Here we present our efforts to perform real-time quantitative optical imaging through a rigid endoscope using Single Snapshot of Optical Properties (SSOP) imaging. In particular, in this work we introduce for the first time 3 dimensionally-corrected dual wavelength optical properties imaging using SSOP through an endoscope, allowing accurate oxygenation maps to be obtained on tissue simulating phantoms and in vivo samples. We compared the results with state-of-the-art wide-field spatial frequency domain imaging (SFDI). Overall, results from the novel endoscopic imaging system agreed within 10% in absorption, reduced scattering, and oxygenation. Moreover, we introduce here real-time, video-rate quantitative optical imaging with 3D profile correction through an endoscope. These results demonstrate the potential of endoscopic SSOP as an objective surgical guidance tool for the clinic.

  8. Pupil Tracking for Real-Time Motion Corrected Anterior Segment Optical Coherence Tomography

    PubMed Central

    Carrasco-Zevallos, Oscar M.; Nankivil, Derek; Viehland, Christian; Keller, Brenton; Izatt, Joseph A.

    2016-01-01

    Volumetric acquisition with anterior segment optical coherence tomography (ASOCT) is necessary to obtain accurate representations of the tissue structure and to account for asymmetries of the anterior eye anatomy. Additionally, recent interest in imaging of anterior segment vasculature and aqueous humor flow resulted in application of OCT angiography techniques to generate en face and 3D micro-vasculature maps of the anterior segment. Unfortunately, ASOCT structural and vasculature imaging systems do not capture volumes instantaneously and are subject to motion artifacts due to involuntary eye motion that may hinder their accuracy and repeatability. Several groups have demonstrated real-time tracking for motion-compensated in vivo OCT retinal imaging, but these techniques are not applicable in the anterior segment. In this work, we demonstrate a simple and low-cost pupil tracking system integrated into a custom swept-source OCT system for real-time motion-compensated anterior segment volumetric imaging. Pupil oculography hardware coaxial with the swept-source OCT system enabled fast detection and tracking of the pupil centroid. The pupil tracking ASOCT system with a field of view of 15 x 15 mm achieved diffraction-limited imaging over a lateral tracking range of +/- 2.5 mm and was able to correct eye motion at up to 22 Hz. Pupil tracking ASOCT offers a novel real-time motion compensation approach that may facilitate accurate and reproducible anterior segment imaging. PMID:27574800

  9. Real-time auto-adaptive margin generation for MLC-tracked radiotherapy.

    PubMed

    Glitzner, M; Fast, M F; de Senneville, B Denis; Nill, S; Oelfke, U; Lagendijk, J J W; Raaymakers, B W; Crijns, S P M

    2017-01-07

    In radiotherapy, abdominal and thoracic sites are candidates for performing motion tracking. With real-time control it is possible to adjust the multileaf collimator (MLC) position to the target position. However, positions are not perfectly matched and position errors arise from system delays and complicated response of the electromechanic MLC system. Although, it is possible to compensate parts of these errors by using predictors, residual errors remain and need to be compensated to retain target coverage. This work presents a method to statistically describe tracking errors and to automatically derive a patient-specific, per-segment margin to compensate the arising underdosage on-line, i.e. during plan delivery. The statistics of the geometric error between intended and actual machine position are derived using kernel density estimators. Subsequently a margin is calculated on-line according to a selected coverage parameter, which determines the amount of accepted underdosage. The margin is then applied onto the actual segment to accommodate the positioning errors in the enlarged segment. The proof-of-concept was tested in an on-line tracking experiment and showed the ability to recover underdosages for two test cases, increasing [Formula: see text] in the underdosed area about [Formula: see text] and [Formula: see text], respectively. The used dose model was able to predict the loss of dose due to tracking errors and could be used to infer the necessary margins. The implementation had a running time of 23 ms which is compatible with real-time requirements of MLC tracking systems. The auto-adaptivity to machine and patient characteristics makes the technique a generic yet intuitive candidate to avoid underdosages due to MLC tracking errors.

  10. Real-time auto-adaptive margin generation for MLC-tracked radiotherapy

    NASA Astrophysics Data System (ADS)

    Glitzner, M.; Fast, M. F.; de Senneville, B. Denis; Nill, S.; Oelfke, U.; Lagendijk, J. J. W.; Raaymakers, B. W.; Crijns, S. P. M.

    2017-01-01

    In radiotherapy, abdominal and thoracic sites are candidates for performing motion tracking. With real-time control it is possible to adjust the multileaf collimator (MLC) position to the target position. However, positions are not perfectly matched and position errors arise from system delays and complicated response of the electromechanic MLC system. Although, it is possible to compensate parts of these errors by using predictors, residual errors remain and need to be compensated to retain target coverage. This work presents a method to statistically describe tracking errors and to automatically derive a patient-specific, per-segment margin to compensate the arising underdosage on-line, i.e. during plan delivery. The statistics of the geometric error between intended and actual machine position are derived using kernel density estimators. Subsequently a margin is calculated on-line according to a selected coverage parameter, which determines the amount of accepted underdosage. The margin is then applied onto the actual segment to accommodate the positioning errors in the enlarged segment. The proof-of-concept was tested in an on-line tracking experiment and showed the ability to recover underdosages for two test cases, increasing {{V}90 %} in the underdosed area about 47 % and 41 % , respectively. The used dose model was able to predict the loss of dose due to tracking errors and could be used to infer the necessary margins. The implementation had a running time of 23 ms which is compatible with real-time requirements of MLC tracking systems. The auto-adaptivity to machine and patient characteristics makes the technique a generic yet intuitive candidate to avoid underdosages due to MLC tracking errors.

  11. Evaluating real-time image reconstruction in diffuse optical tomography using physiologically realistic test data.

    PubMed

    Brigadoi, Sabrina; Powell, Samuel; Cooper, Robert J; Dempsey, Laura A; Arridge, Simon; Everdell, Nick; Hebden, Jeremy; Gibson, Adam P

    2015-12-01

    In diffuse optical tomography (DOT), real-time image reconstruction of oxy- and deoxy-haemoglobin changes occurring in the brain could give valuable information in clinical care settings. Although non-linear reconstruction techniques could provide more accurate results, their computational burden makes them unsuitable for real-time applications. Linear techniques can be employed under the assumption that the expected change in absorption is small. Several approaches exist, differing primarily in their handling of regularization and the noise statistics. In real experiments, it is impossible to compute the true noise statistics, because of the presence of physiological oscillations in the measured data. This is even more critical in real-time applications, where no off-line filtering and averaging can be performed to reduce the noise level. Therefore, many studies substitute the noise covariance matrix with the identity matrix. In this paper, we examined two questions: does using the noise model with realistic, imperfect data yield an improvement in image quality compared to using the identity matrix; and what is the difference in quality between online and offline reconstructions. Bespoke test data were created using a novel process through which simulated changes in absorption were added to real resting-state DOT data. A realistic multi-layer head model was used as the geometry for the reconstruction. Results validated our assumptions, highlighting the validity of computing the noise statistics from the measured data for online image reconstruction, which was performed at 2 Hz. Our results can be directly extended to a real application where real-time imaging is required.

  12. Real-time optical multiple object recognition and tracking system and method

    NASA Technical Reports Server (NTRS)

    Chao, Tien-Hsin (Inventor); Liu, Hua Kuang (Inventor)

    1987-01-01

    The invention relates to an apparatus and associated methods for the optical recognition and tracking of multiple objects in real time. Multiple point spatial filters are employed that pre-define the objects to be recognized at run-time. The system takes the basic technology of a Vander Lugt filter and adds a hololens. The technique replaces time, space and cost-intensive digital techniques. In place of multiple objects, the system can also recognize multiple orientations of a single object. This later capability has potential for space applications where space and weight are at a premium.

  13. Digital signal processor-based real-time optical Doppler tomography system.

    PubMed

    Yan, Shikui; Piao, Daqing; Chen, Yueli; Zhu, Quing

    2004-01-01

    We present a real-time data-processing and display unit based on a custom-designed digital signal processor (DSP) module for imaging tissue structure and Doppler blood flow. The DSP module is incorporated into a conventional optical coherence tomography system. We also demonstrate the flexibility of embedding advanced Doppler processing algorithms in the DSP module. Two advanced velocity estimation algorithms previously introduced by us are incorporated in this DSP module. Experiments on Intralipid flow demonstrate that a pulsatile flow of several hundred pulses per minute can be faithfully captured in M-scan mode by this DSP system. In vivo imaging of a rat's abdominal blood flow is also presented.

  14. Real time assessment of RF cardiac tissue ablation with optical spectroscopy

    SciTech Connect

    Demos, S G; Sharareh, S

    2008-03-20

    An optical spectroscopy approach is demonstrated allowing for critical parameters during RF ablation of cardiac tissue to be evaluated in real time. The method is based on incorporating in a typical ablation catheter transmitting and receiving fibers that terminate at the tip of the catheter. By analyzing the spectral characteristics of the NIR diffusely reflected light, information is obtained on such parameters as, catheter-tissue proximity, lesion formation, depth of penetration of the lesion, formation of char during the ablation, formation of coagulum around the ablation site, differentiation of ablated from healthy tissue, and recognition of micro-bubble formation in the tissue.

  15. Real-time optical multiple object recognition and tracking system and method

    NASA Astrophysics Data System (ADS)

    Chao, Tien-Hsin; Liu, Hua Kuang

    1987-12-01

    The invention relates to an apparatus and associated methods for the optical recognition and tracking of multiple objects in real time. Multiple point spatial filters are employed that pre-define the objects to be recognized at run-time. The system takes the basic technology of a Vander Lugt filter and adds a hololens. The technique replaces time, space and cost-intensive digital techniques. In place of multiple objects, the system can also recognize multiple orientations of a single object. This later capability has potential for space applications where space and weight are at a premium.

  16. Real-Time FPGA Processing for High-Speed Optical Frequency Domain Imaging

    PubMed Central

    Vakoc, Benjamin J.; Suter, Melissa J.; Yun, Seok-Hyun; Tearney, Guillermo J.; Bouma, Brett E.

    2010-01-01

    We present a novel algorithm for reconstructing interferograms acquired in optical frequency domain imaging (OFDI). The algorithm was developed specifically for processing in field programmable gate arrays (FPGAs) and featured the use of a finite-impulse-response (FIR) filter implementation of B-spline interpolation for efficiently re-sampling k-space. When implemented in FPGAs, the algorithm allowed for real-time processing of interferograms acquired with a high-speed OFDI system at 54 kHz and a sampling rate of 100 MS/s. PMID:19336296

  17. Real-time VanderLugt optical correlator that uses photorefractive GaAs

    NASA Technical Reports Server (NTRS)

    Liu, Duncan T. H.; Cheng, Li-Jen

    1992-01-01

    A real-time GaAs and liquid-crystal television- (LCTV-) based optical correlator is demonstrated. The speed of this correlator (video rate) is limited by the LCTV's; with faster spatial light modulators, the potential speed of a GaAs-based correlator may be 1000 frames/s or higher. Comparisons are made between VanderLugt and joint transform configurations and between degenerate and nondegenerate four-wave mixings. The edge-enhancement effect and the Bragg diffraction effect are also discussed.

  18. Optical Response of Warm Dense Matter Using Real-Time Electron Dynamics

    NASA Astrophysics Data System (ADS)

    Baczewski, Andrew; Shulenburger, Luke; Desjarlais, Michael; Magyar, Rudolph

    2014-03-01

    The extreme temperatures and solid-like densities in warm dense matter present a unique challenge for theory, wherein neither conventional models from condensed matter nor plasma physics capture all of the relevant phenomenology. While Kubo-Greenwood DFT calculations have proven capable of reproducing optical properties of WDM, they require a significant number of virtual orbitals to reach convergence due to their perturbative nature. Real-time TDDFT presents a complementary framework with a number of computationally favorable properties, including reduced cost complexity and better scalability, and has been used to reproduce the optical response of finite and ordered extended systems. We will describe the use of Ehrenfest-TDDFT to evolve coupled electron-nuclear dynamics in WDM systems, and the subsequent evaluation of optical response functions from the real-time electron dynamics. The advantages and disadvantages of this approach will be discussed relative to the current state-of-the-art. Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Security Administration under contract DE-AC04-94AL85000.

  19. Real-time adaptive microstimulation increases reliability of electrically evoked cortical potentials.

    PubMed

    Brugger, Dominik; Butovas, Sergejus; Bogdan, Martin; Schwarz, Cornelius

    2011-05-01

    Cortical neuroprostheses that employ repeated electrical stimulation of cortical areas with fixed stimulus parameters, are faced with the problem of large trial-by-trial variability of evoked potentials. This variability is caused by the ongoing cortical signal processing, but it is an unwanted phenomenon if one aims at imprinting neural activity as precisely as possible. Here, we use local field potentials measured by one microelectrode, located at a distance of 200 microns from the stimulation site, to drive the electrically evoked potential toward a desired target potential by real-time adaptation of the stimulus intensity. The functional relationship between ongoing cortical activity, evoked potential, and stimulus intensity was estimated by standard machine learning techniques (support vector regression with problem-specific kernel function) from a set of stimulation trials with randomly varied stimulus intensities. The smallest deviation from the target potential was achieved for low stimulus intensities. Further, the observed precision effect proved time sensitive, since it was abolished by introducing a delay between data acquisition and stimulation. These results indicate that local field potentials contain sufficient information about ongoing local signal processing to stabilize electrically evoked potentials. We anticipate that adaptive low intensity microstimulation will play an important role in future cortical prosthetic devices that aim at restoring lost sensory functions.

  20. Real-time measurements of spontaneous breathers and rogue wave events in optical fibre modulation instability

    PubMed Central

    Närhi, Mikko; Wetzel, Benjamin; Billet, Cyril; Toenger, Shanti; Sylvestre, Thibaut; Merolla, Jean-Marc; Morandotti, Roberto; Dias, Frederic; Genty, Goëry; Dudley, John M.

    2016-01-01

    Modulation instability is a fundamental process of nonlinear science, leading to the unstable breakup of a constant amplitude solution of a physical system. There has been particular interest in studying modulation instability in the cubic nonlinear Schrödinger equation, a generic model for a host of nonlinear systems including superfluids, fibre optics, plasmas and Bose–Einstein condensates. Modulation instability is also a significant area of study in the context of understanding the emergence of high amplitude events that satisfy rogue wave statistical criteria. Here, exploiting advances in ultrafast optical metrology, we perform real-time measurements in an optical fibre system of the unstable breakup of a continuous wave field, simultaneously characterizing emergent modulation instability breather pulses and their associated statistics. Our results allow quantitative comparison between experiment, modelling and theory, and are expected to open new perspectives on studies of instability dynamics in physics. PMID:27991513

  1. Real-time measurements of spontaneous breathers and rogue wave events in optical fibre modulation instability

    NASA Astrophysics Data System (ADS)

    Närhi, Mikko; Wetzel, Benjamin; Billet, Cyril; Toenger, Shanti; Sylvestre, Thibaut; Merolla, Jean-Marc; Morandotti, Roberto; Dias, Frederic; Genty, Goëry; Dudley, John M.

    2016-12-01

    Modulation instability is a fundamental process of nonlinear science, leading to the unstable breakup of a constant amplitude solution of a physical system. There has been particular interest in studying modulation instability in the cubic nonlinear Schrödinger equation, a generic model for a host of nonlinear systems including superfluids, fibre optics, plasmas and Bose-Einstein condensates. Modulation instability is also a significant area of study in the context of understanding the emergence of high amplitude events that satisfy rogue wave statistical criteria. Here, exploiting advances in ultrafast optical metrology, we perform real-time measurements in an optical fibre system of the unstable breakup of a continuous wave field, simultaneously characterizing emergent modulation instability breather pulses and their associated statistics. Our results allow quantitative comparison between experiment, modelling and theory, and are expected to open new perspectives on studies of instability dynamics in physics.

  2. Real-time near-field terahertz imaging with atomic optical fluorescence

    NASA Astrophysics Data System (ADS)

    Wade, C. G.; Šibalić, N.; de Melo, N. R.; Kondo, J. M.; Adams, C. S.; Weatherill, K. J.

    2017-01-01

    Terahertz (THz) near-field imaging is a flourishing discipline, with applications from fundamental studies of beam propagation to the characterization of metamaterials and waveguides. Beating the diffraction limit typically involves rastering structures or detectors with length scale shorter than the radiation wavelength; in the THz domain this has been achieved using a number of techniques including scattering tips and apertures. Alternatively, mapping THz fields onto an optical wavelength and imaging the visible light removes the requirement for scanning a local probe, speeding up image collection times. Here, we report THz-to-optical conversion using a gas of highly excited Rydberg atoms. By collecting THz-induced optical fluorescence we demonstrate a real-time image of a THz standing wave and use well-known atomic properties to calibrate the THz field strength.

  3. Real-time measurements of spontaneous breathers and rogue wave events in optical fibre modulation instability.

    PubMed

    Närhi, Mikko; Wetzel, Benjamin; Billet, Cyril; Toenger, Shanti; Sylvestre, Thibaut; Merolla, Jean-Marc; Morandotti, Roberto; Dias, Frederic; Genty, Goëry; Dudley, John M

    2016-12-19

    Modulation instability is a fundamental process of nonlinear science, leading to the unstable breakup of a constant amplitude solution of a physical system. There has been particular interest in studying modulation instability in the cubic nonlinear Schrödinger equation, a generic model for a host of nonlinear systems including superfluids, fibre optics, plasmas and Bose-Einstein condensates. Modulation instability is also a significant area of study in the context of understanding the emergence of high amplitude events that satisfy rogue wave statistical criteria. Here, exploiting advances in ultrafast optical metrology, we perform real-time measurements in an optical fibre system of the unstable breakup of a continuous wave field, simultaneously characterizing emergent modulation instability breather pulses and their associated statistics. Our results allow quantitative comparison between experiment, modelling and theory, and are expected to open new perspectives on studies of instability dynamics in physics.

  4. Experimental Results: Detection and Tracking of Low SNR Sinusoids Using Real-Time LMS and RLS Lattice Adaptive Line Enhancers.

    DTIC Science & Technology

    1991-08-01

    DETECTION AND TRACKING OF LOW SNR SINUSOIDS USING REAL-TIMNE LMS AND RI S LATTIICE, ADAPTIVE LINE PR: SSWh ENHANCRS RE: 0300000liN 6 AIJTHCRISI WVl: D68...RESULTS: DETECTION AND TRACKING OF LOW SNR SINUSOIDS USING REAL-TIME LMS AND RLS LATTICE ADAPTIVE LINE ENHANCERS i f. Terence R. Albert, Hana Abusalem...obtained from a real-time custom hardware SNR sinusoids and filter parameters such as system using 32-bit IEEE floating point filter length, and adaption

  5. Real-time feature extraction of P300 component using adaptive nonlinear principal component analysis

    PubMed Central

    2011-01-01

    Background The electroencephalography (EEG) signals are known to involve the firings of neurons in the brain. The P300 wave is a high potential caused by an event-related stimulus. The detection of P300s included in the measured EEG signals is widely investigated. The difficulties in detecting them are that they are mixed with other signals generated over a large brain area and their amplitudes are very small due to the distance and resistivity differences in their transmittance. Methods A novel real-time feature extraction method for detecting P300 waves by combining an adaptive nonlinear principal component analysis (ANPCA) and a multilayer neural network is proposed. The measured EEG signals are first filtered using a sixth-order band-pass filter with cut-off frequencies of 1 Hz and 12 Hz. The proposed ANPCA scheme consists of four steps: pre-separation, whitening, separation, and estimation. In the experiment, four different inter-stimulus intervals (ISIs) are utilized: 325 ms, 350 ms, 375 ms, and 400 ms. Results The developed multi-stage principal component analysis method applied at the pre-separation step has reduced the external noises and artifacts significantly. The introduced adaptive law in the whitening step has made the subsequent algorithm in the separation step to converge fast. The separation performance index has varied from -20 dB to -33 dB due to randomness of source signals. The robustness of the ANPCA against background noises has been evaluated by comparing the separation performance indices of the ANPCA with four algorithms (NPCA, NSS-JD, JADE, and SOBI), in which the ANPCA algorithm demonstrated the shortest iteration time with performance index about 0.03. Upon this, it is asserted that the ANPCA algorithm successfully separates mixed source signals. Conclusions The independent components produced from the observed data using the proposed method illustrated that the extracted signals were clearly the P300 components elicited by task

  6. A Portable Array-Type Optical Fiber Sensing Instrument for Real-Time Gas Detection.

    PubMed

    Hung, San-Shan; Chang, Hsing-Cheng; Chang, I-Nan

    2016-12-08

    A novel optical fiber array-type of sensing instrument with temperature compensation for real-time detection was developed to measure oxygen, carbon dioxide, and ammonia simultaneously. The proposed instrument is multi-sensing array integrated with real-time measurement module for portable applications. The sensing optical fibers were etched and polished before coating to increase sensitivities. The ammonia and temperature sensors were each composed of a dye-coated single-mode fiber with constructing a fiber Bragg grating and a long-period filter grating for detecting light intensity. Both carbon dioxide and oxygen sensing structures use multimode fibers where 1-hydroxy-3,6,8-pyrene trisulfonic acid trisodium salt is coated for carbon dioxide sensing and Tris(2,2'-bipyridyl) dichlororuthenium(II) hexahydrate and Tris(bipyridine)ruthenium(II) chloride are coated for oxygen sensing. Gas-induced fluorescent light intensity variation was applied to detect gas concentration. The portable gas sensing array was set up by integrating with photo-electronic measurement modules and a human-machine interface to detect gases in real time. The measured data have been processed using piecewise-linear method. The sensitivity of the oxygen sensor were 1.54%/V and 9.62%/V for concentrations less than 1.5% and for concentrations between 1.5% and 6%, respectively. The sensitivity of the carbon dioxide sensor were 8.33%/V and 9.62%/V for concentrations less than 2% and for concentrations between 2% and 5%, respectively. For the ammonia sensor, the sensitivity was 27.78%/V, while ammonia concentration was less than 2%.

  7. A Portable Array-Type Optical Fiber Sensing Instrument for Real-Time Gas Detection

    PubMed Central

    Hung, San-Shan; Chang, Hsing-Cheng; Chang, I-Nan

    2016-01-01

    A novel optical fiber array-type of sensing instrument with temperature compensation for real-time detection was developed to measure oxygen, carbon dioxide, and ammonia simultaneously. The proposed instrument is multi-sensing array integrated with real-time measurement module for portable applications. The sensing optical fibers were etched and polished before coating to increase sensitivities. The ammonia and temperature sensors were each composed of a dye-coated single-mode fiber with constructing a fiber Bragg grating and a long-period filter grating for detecting light intensity. Both carbon dioxide and oxygen sensing structures use multimode fibers where 1-hydroxy-3,6,8-pyrene trisulfonic acid trisodium salt is coated for carbon dioxide sensing and Tris(2,2′-bipyridyl) dichlororuthenium(II) hexahydrate and Tris(bipyridine)ruthenium(II) chloride are coated for oxygen sensing. Gas-induced fluorescent light intensity variation was applied to detect gas concentration. The portable gas sensing array was set up by integrating with photo-electronic measurement modules and a human-machine interface to detect gases in real time. The measured data have been processed using piecewise-linear method. The sensitivity of the oxygen sensor were 1.54%/V and 9.62%/V for concentrations less than 1.5% and for concentrations between 1.5% and 6%, respectively. The sensitivity of the carbon dioxide sensor were 8.33%/V and 9.62%/V for concentrations less than 2% and for concentrations between 2% and 5%, respectively. For the ammonia sensor, the sensitivity was 27.78%/V, while ammonia concentration was less than 2%. PMID:27941636

  8. A New Approach to Interference Excision in Radio Astronomy: Real-Time Adaptive Cancellation

    NASA Astrophysics Data System (ADS)

    Barnbaum, Cecilia; Bradley, Richard F.

    1998-11-01

    Every year, an increasing amount of radio-frequency (RF) spectrum in the VHF, UHF, and microwave bands is being utilized to support new commercial and military ventures, and all have the potential to interfere with radio astronomy observations. Such services already cause problems for radio astronomy even in very remote observing sites, and the potential for this form of light pollution to grow is alarming. Preventive measures to eliminate interference through FCC legislation and ITU agreements can be effective; however, many times this approach is inadequate and interference excision at the receiver is necessary. Conventional techniques such as RF filters, RF shielding, and postprocessing of data have been only somewhat successful, but none has been sufficient. Adaptive interference cancellation is a real-time approach to interference excision that has not been used before in radio astronomy. We describe here, for the first time, adaptive interference cancellation in the context of radio astronomy instrumentation, and we present initial results for our prototype receiver. In the 1960s, analog adaptive interference cancelers were developed that obtain a high degree of cancellation in problems of radio communications and radar. However, analog systems lack the dynamic range, noised performance, and versatility required by radio astronomy. The concept of digital adaptive interference cancellation was introduced in the mid-1960s as a way to reduce unwanted noise in low-frequency (audio) systems. Examples of such systems include the canceling of maternal ECG in fetal electrocardiography and the reduction of engine noise in the passenger compartments of automobiles. These audio-frequency applications require bandwidths of only a few tens of kilohertz. Only recently has high-speed digital filter technology made high dynamic range adaptive canceling possible in a bandwidth as large as a few megahertz, finally opening the door to application in radio astronomy. We have

  9. Real-time distortion correction system of large-field optical display equipment based on FPGA

    NASA Astrophysics Data System (ADS)

    Zheng, Chun; Zhou, Yongjun; Zheng, Yongrui; Li, Jie

    2011-08-01

    Current distortion correction systems can not meet the requirements of the large-field optical display equipment because of small field, low resolution, poor real-time property and commonality. "The symmetrical transform" and "the improved bilinear interpolation" were proposed. The general system scheme was designed and implemented in the Virtex-5 FPGA devices. The appropriate data structure of the look-up table was adopted and the optimized scheme for the input memory named "the double even-odd cache" was put forward. MIG (Memory Interface Generator) software tool was utilized to control DDR2 SDRAM and DSP48E was used. The real-time distortion correction system of the large-field optical display equipment was accomplished. The experimental result shows that the correction system can correct the large-field and high-resolution (1280x1024) video image (60 frames per second). The system delays only 1.48ms while the deviation in precision is less than 9' and has the well commonality.

  10. Ranque-Hilsch vortex tube thermocycler for fast DNA amplification and real-time optical detection

    NASA Astrophysics Data System (ADS)

    Ebmeier, Ryan J.; Whitney, Scott E.; Sarkar, Amitabha; Nelson, Michael; Padhye, Nisha V.; Gogos, George; Viljoen, Hendrik J.

    2004-12-01

    An innovative polymerase chain reaction (PCR) thermocycler capable of performing real-time optical detection is described below. This device utilizes the Ranque-Hilsch vortex tube in a system to efficiently and rapidly cycle three 20 μL samples between the denaturation, annealing, and elongation temperatures. The reaction progress is displayed real-time by measuring the size of a fluorescent signal emitted by SYBR green/double-stranded DNA complexes. This device can produce significant reaction yields with very small amounts of initial DNA, for example, it can amplify 0.25 fg (˜5 copies) of a 96 bp bacteriophage λ-DNA fragment 2.7×1011-fold by performing 45 cycles in less than 12 min. The optical threshold (150% of the baseline intensity) was passed 8 min into the reaction at cycle 34. Besides direct applications, the speed and sensitivity of this device enables it to be used as a scientific instrument for basic studies such as PCR assembly and polymerase kinetics.

  11. Optical fibre luminescence sensor for real-time LDR brachytherapy dosimetry

    NASA Astrophysics Data System (ADS)

    Woulfe, P.; Sullivan, F. J.; O'Keeffe, S.

    2016-05-01

    An optical fibre sensor for monitoring low dose radiation is presented. The sensor is based on a scintillation material embedded within the optical fibre core, which emits visible light when exposed to low level ionising radiation. The incident level of ionising radiation can be determined by analysing the optical emission. An optical fibre sensor is presented, based on radioluminescence whereby radiation sensitive scintillation material, terbium doped gadolinium oxysulphide (Gd2O2S:Tb), is embedded in a cavity of 250μm of a 500μm plastic optical fibre. The sensor is designed for in-vivo monitoring of the radiation dose during radio-active seed implantation for brachytherapy, in prostate cancer treatment, providing oncologists with real-time information of the radiation dose to the target area and/or nearby critical structures. The radiation from the brachytherapy seeds causes emission of visible light from the scintillation material through the process of radioluminescence, which penetrates the fibre, propagating along the optical fibre for remote detection using a multi-pixel photon counter. The sensor demonstrates a high sensitivity to Iodine-125, the radioactive source most commonly used in brachytherapy for treating prostate cancer.

  12. Motion-adapted catheter navigation with real-time instantiation and improved visualisation.

    PubMed

    Lee, Su-Lin; Kwok, Ka-Wai; Wang, Lichao; Riga, Celia; Bicknell, Colin; Cheshire, Nicholas; Yang, Guang-Zhong

    2013-09-01

    The improvements to catheter manipulation by the use of robot-assisted catheter navigation for endovascular procedures include increased precision, stability of motion and operator comfort. However, navigation through the vasculature under fluoroscopic guidance is still challenging, mostly due to physiological motion and when tortuous vessels are involved. In this paper, we propose a motion-adaptive catheter navigation scheme based on shape modelling to compensate for these dynamic effects, permitting predictive and dynamic navigations. This allows for timed manipulations synchronised with the vascular motion. The technical contribution of the paper includes the following two aspects. Firstly, a dynamic shape modelling and real-time instantiation scheme based on sparse data obtained intra-operatively is proposed for improved visualisation of the 3D vasculature during endovascular intervention. Secondly, a reconstructed frontal view from the catheter tip using the derived dynamic model is used as an interventional aid to user guidance. To demonstrate the practical value of the proposed framework, a simulated aortic branch cannulation procedure is used with detailed user validation to demonstrate the improvement in navigation quality and efficiency.

  13. Real-Time Reconfigurable Adaptive Speech Recognition Command and Control Apparatus and Method

    NASA Technical Reports Server (NTRS)

    Salazar, George A. (Inventor); Haynes, Dena S. (Inventor); Sommers, Marc J. (Inventor)

    1998-01-01

    An adaptive speech recognition and control system and method for controlling various mechanisms and systems in response to spoken instructions and in which spoken commands are effective to direct the system into appropriate memory nodes, and to respective appropriate memory templates corresponding to the voiced command is discussed. Spoken commands from any of a group of operators for which the system is trained may be identified, and voice templates are updated as required in response to changes in pronunciation and voice characteristics over time of any of the operators for which the system is trained. Provisions are made for both near-real-time retraining of the system with respect to individual terms which are determined not be positively identified, and for an overall system training and updating process in which recognition of each command and vocabulary term is checked, and in which the memory templates are retrained if necessary for respective commands or vocabulary terms with respect to an operator currently using the system. In one embodiment, the system includes input circuitry connected to a microphone and including signal processing and control sections for sensing the level of vocabulary recognition over a given period and, if recognition performance falls below a given level, processing audio-derived signals for enhancing recognition performance of the system.

  14. Adaptive neuro-fuzzy inference system for real-time monitoring of integrated-constructed wetlands.

    PubMed

    Dzakpasu, Mawuli; Scholz, Miklas; McCarthy, Valerie; Jordan, Siobhán; Sani, Abdulkadir

    2015-01-01

    Monitoring large-scale treatment wetlands is costly and time-consuming, but required by regulators. Some analytical results are available only after 5 days or even longer. Thus, adaptive neuro-fuzzy inference system (ANFIS) models were developed to predict the effluent concentrations of 5-day biochemical oxygen demand (BOD5) and NH4-N from a full-scale integrated constructed wetland (ICW) treating domestic wastewater. The ANFIS models were developed and validated with a 4-year data set from the ICW system. Cost-effective, quicker and easier to measure variables were selected as the possible predictors based on their goodness of correlation with the outputs. A self-organizing neural network was applied to extract the most relevant input variables from all the possible input variables. Fuzzy subtractive clustering was used to identify the architecture of the ANFIS models and to optimize fuzzy rules, overall, improving the network performance. According to the findings, ANFIS could predict the effluent quality variation quite strongly. Effluent BOD5 and NH4-N concentrations were predicted relatively accurately by other effluent water quality parameters, which can be measured within a few hours. The simulated effluent BOD5 and NH4-N concentrations well fitted the measured concentrations, which was also supported by relatively low mean squared error. Thus, ANFIS can be useful for real-time monitoring and control of ICW systems.

  15. Refinement and evaluation of helicopter real-time self-adaptive active vibration controller algorithms

    NASA Technical Reports Server (NTRS)

    Davis, M. W.

    1984-01-01

    A Real-Time Self-Adaptive (RTSA) active vibration controller was used as the framework in developing a computer program for a generic controller that can be used to alleviate helicopter vibration. Based upon on-line identification of system parameters, the generic controller minimizes vibration in the fuselage by closed-loop implementation of higher harmonic control in the main rotor system. The new generic controller incorporates a set of improved algorithms that gives the capability to readily define many different configurations by selecting one of three different controller types (deterministic, cautious, and dual), one of two linear system models (local and global), and one or more of several methods of applying limits on control inputs (external and/or internal limits on higher harmonic pitch amplitude and rate). A helicopter rotor simulation analysis was used to evaluate the algorithms associated with the alternative controller types as applied to the four-bladed H-34 rotor mounted on the NASA Ames Rotor Test Apparatus (RTA) which represents the fuselage. After proper tuning all three controllers provide more effective vibration reduction and converge more quickly and smoothly with smaller control inputs than the initial RTSA controller (deterministic with external pitch-rate limiting). It is demonstrated that internal limiting of the control inputs a significantly improves the overall performance of the deterministic controller.

  16. Cell Models Adapted to Real-Time Imaging of the Cytoskeleton Dynamics in Altered Gravity

    NASA Astrophysics Data System (ADS)

    Willems, Jérôme; Deroanne, Christophe; Colige, Alain; Garbacki, Nancy

    2014-11-01

    Spatial and temporal regulation of cell phenotype by mechanical forces is a growing field of research in health sciences since these stimuli influence cellular functions, such as proliferation, migration, differentiation and gene expression. In the context of the Fluolive project selected by the European Space Agency and aiming at evaluating the impact of gravity alterations on the cell phenotype, we have developed new bone-derived cell lines adapted for live-cell imaging of the cytoskeleton. Osteoblastic cells derived from human osteosarcomas were used as experimental models. U2-OS and SaoS-2 cells stably expressing TagGFP2- β-actin and mCherry- α-tubulin were established and single-cell clonal cultures were characterized in terms of recombinant proteins production and localization, fluorescence intensity, cell proliferation and migration rates. Living fluorescently-tagged cell lines allow real-time fluorescence microscopy of the cytoskeleton dynamics without bleaching and without alteration of cell morphology. U2-OS and SaoS-2 TagGFP2- β-actin and mCherry- α-tubulin clones will be used to monitor the effect of mechanical forces in models of altered gravity on Earth and possibly on the ISS.

  17. Real-time nutrient monitoring in rivers: adaptive sampling strategies, technological challenges and future directions

    NASA Astrophysics Data System (ADS)

    Blaen, Phillip; Khamis, Kieran; Lloyd, Charlotte; Bradley, Chris

    2016-04-01

    Excessive nutrient concentrations in river waters threaten aquatic ecosystem functioning and can pose substantial risks to human health. Robust monitoring strategies are therefore required to generate reliable estimates of river nutrient loads and to improve understanding of the catchment processes that drive spatiotemporal patterns in nutrient fluxes. Furthermore, these data are vital for prediction of future trends under changing environmental conditions and thus the development of appropriate mitigation measures. In recent years, technological developments have led to an increase in the use of continuous in-situ nutrient analysers, which enable measurements at far higher temporal resolutions than can be achieved with discrete sampling and subsequent laboratory analysis. However, such instruments can be costly to run and difficult to maintain (e.g. due to high power consumption and memory requirements), leading to trade-offs between temporal and spatial monitoring resolutions. Here, we highlight how adaptive monitoring strategies, comprising a mixture of temporal sample frequencies controlled by one or more 'trigger variables' (e.g. river stage, turbidity, or nutrient concentration), can advance our understanding of catchment nutrient dynamics while simultaneously overcoming many of the practical and economic challenges encountered in typical in-situ river nutrient monitoring applications. We present examples of short-term variability in river nutrient dynamics, driven by complex catchment behaviour, which support our case for the development of monitoring systems that can adapt in real-time to rapid environmental changes. In addition, we discuss the advantages and disadvantages of current nutrient monitoring techniques, and suggest new research directions based on emerging technologies and highlight how these might improve: 1) monitoring strategies, and 2) understanding of linkages between catchment processes and river nutrient fluxes.

  18. Real-time detection of lipid bilayer assembly and detergent-initiated solubilization using optical cavities

    NASA Astrophysics Data System (ADS)

    Sun, V.; Armani, A. M.

    2015-02-01

    The cellular membrane governs numerous fundamental biological processes. Therefore, developing a comprehensive understanding of its structure and function is critical. However, its inherent biological complexity gives rise to numerous inter-dependent physical phenomena. In an attempt to develop a model, two different experimental approaches are being pursued in parallel: performing single cell experiments (top down) and using biomimetic structures (bottom up), such as lipid bilayers. One challenge in many of these experiments is the reliance on fluorescent probes for detection which can create confounds in this already complex system. In the present work, a label-free detection method based on an optical resonant cavity is used to detect one of the fundamental physical phenomena in the system: assembly and solubilization of the lipid bilayer. The evanescent field of the cavity strongly interacts with the lipid bilayer, enabling the detection of the bilayer behavior in real-time. Two independent detection mechanisms confirm the formation and detergent-assisted solubilization of the lipid bilayers: (1) a refractive index change and (2) a material loss change. Both mechanisms can be monitored in parallel, on the same device, thus allowing for cross-confirmation of the results. To verify the proposed method, we have detected the formation of self-assembled phosphatidylcholine lipid bilayers from small unilamellar vesicles on the device surface in real-time. Subsequently, we exposed the bilayers to two different detergents (non-ionic Triton X-100 and anionic sodium dodecyl sulfate) to initiate solubilization, and this process was also detected in real-time. After the bilayer solubilization, the device returned to its initial state, exhibiting minimal hysteresis. The experimental wash-off was also collected and analyzed using dynamic light scattering.

  19. Emerging fiber optic endomicroscopy technologies towards noninvasive real-time visualization of histology in situ

    NASA Astrophysics Data System (ADS)

    Xi, Jiefeng; Zhang, Yuying; Huo, Li; Chen, Yongping; Jabbour, Toufic; Li, Ming-Jun; Li, Xingde

    2010-09-01

    This paper reviews our recent developments of ultrathin fiber-optic endomicroscopy technologies for transforming high-resolution noninvasive optical imaging techniques to in vivo and clinical applications such as early disease detection and guidance of interventions. Specifically we describe an all-fiber-optic scanning endomicroscopy technology, which miniaturizes a conventional bench-top scanning laser microscope down to a flexible fiber-optic probe of a small footprint (i.e. ~2-2.5 mm in diameter), capable of performing two-photon fluorescence and second harmonic generation microscopy in real time. This technology aims to enable realtime visualization of histology in situ without the need for tissue removal. We will also present a balloon OCT endoscopy technology which permits high-resolution 3D imaging of the entire esophagus for detection of neoplasia, guidance of biopsy and assessment of therapeutic outcome. In addition we will discuss the development of functional polymeric fluorescent nanocapsules, which use only FAD approved materials and potentially enable fast track clinical translation of optical molecular imaging and targeted therapy.

  20. Automatic real time evaluation of red blood cell elasticity by optical tweezers

    NASA Astrophysics Data System (ADS)

    Moura, Diógenes S.; Silva, Diego C. N.; Williams, Ajoke J.; Bezerra, Marcos A. C.; Fontes, Adriana; de Araujo, Renato E.

    2015-05-01

    Optical tweezers have been used to trap, manipulate, and measure individual cell properties. In this work, we show that the association of a computer controlled optical tweezers system with image processing techniques allows rapid and reproducible evaluation of cell deformability. In particular, the deformability of red blood cells (RBCs) plays a key role in the transport of oxygen through the blood microcirculation. The automatic measurement processes consisted of three steps: acquisition, segmentation of images, and measurement of the elasticity of the cells. An optical tweezers system was setup on an upright microscope equipped with a CCD camera and a motorized XYZ stage, computer controlled by a Labview platform. On the optical tweezers setup, the deformation of the captured RBC was obtained by moving the motorized stage. The automatic real-time homemade system was evaluated by measuring RBCs elasticity from normal donors and patients with sickle cell anemia. Approximately 150 erythrocytes were examined, and the elasticity values obtained by using the developed system were compared to the values measured by two experts. With the automatic system, there was a significant time reduction (60 × ) of the erythrocytes elasticity evaluation. Automated system can help to expand the applications of optical tweezers in hematology and hemotherapy.

  1. Modelling effluent quality based on a real-time optical monitoring of the wastewater treatment process.

    PubMed

    Tomperi, Jani; Koivuranta, Elisa; Kuokkanen, Anna; Leiviskä, Kauko

    2017-01-01

    A novel optical monitoring device was used for imaging an activated sludge process in situ during a period of over one year. In this study, the dependencies between the results of image analysis and the process measurements were studied, and the optical monitoring results were utilized to predict the important quality parameters for the wastewater treatment process efficiency: suspended solids, biological oxygen demand, chemical oxygen demand, total nitrogen and total phosphorous in biologically treated wastewater. The optimal subsets of variables for each model were searched using five variable selection methods. It was shown that online optical analysis results have clear dependencies on some process variables and the purification result. The model based on optical monitoring and process variables from the early stage of the treatment process can be used to predict the levels of important quality parameters, and to show the quality of the biologically treated wastewater hours in advance. This study confirms that the optical monitoring method is a valuable tool for monitoring a wastewater treatment process and receiving new information in real time. Combined with predictive modelling, it has the potential to be used in process control, keeping the process in a stable operating condition and avoiding environmental risks.

  2. Real-time optical diagnostics of graphene growth induced by pulsed chemical vapor deposition

    NASA Astrophysics Data System (ADS)

    Puretzky, Alexander A.; Geohegan, David B.; Pannala, Sreekanth; Rouleau, Christopher M.; Regmi, Murari; Thonnard, Norbert; Eres, Gyula

    2013-06-01

    The kinetics and mechanisms of graphene growth on Ni films at 720-880 °C have been measured using fast pulses of acetylene and real-time optical diagnostics. In situ UV-Raman spectroscopy was used to unambiguously detect isothermal graphene growth at high temperatures, measure the growth kinetics with ~1 s temporal resolution, and estimate the fractional precipitation upon cooldown. Optical reflectivity and videography provided much faster temporal resolution. Both the growth kinetics and the fractional isothermal precipitation were found to be governed by the C2H2 partial pressure in the CVD pulse for a given film thickness and temperature, with up to ~94% of graphene growth occurring isothermally within 1 second at 800 °C at high partial pressures. At lower partial pressures, isothermal graphene growth is shown to continue 10 seconds after the gas pulse. These flux-dependent growth kinetics are described in the context of a dissolution/precipitation model, where carbon rapidly dissolves into the Ni film and later precipitates driven by gradients in the chemical potential. The combination of pulsed-CVD and real-time optical diagnostics opens new opportunities to understand and control the fast, sub-second growth of graphene on various substrates at high temperatures.The kinetics and mechanisms of graphene growth on Ni films at 720-880 °C have been measured using fast pulses of acetylene and real-time optical diagnostics. In situ UV-Raman spectroscopy was used to unambiguously detect isothermal graphene growth at high temperatures, measure the growth kinetics with ~1 s temporal resolution, and estimate the fractional precipitation upon cooldown. Optical reflectivity and videography provided much faster temporal resolution. Both the growth kinetics and the fractional isothermal precipitation were found to be governed by the C2H2 partial pressure in the CVD pulse for a given film thickness and temperature, with up to ~94% of graphene growth occurring isothermally

  3. Real-time characterization of spectral coherence of ultrafast laser based on optical time-stretch

    NASA Astrophysics Data System (ADS)

    Xu, Yiqing; Wei, Xiaoming; Ren, Zhibo; Wong, Kenneth K. Y.; Tsia, Kevin

    2016-03-01

    Nonlinearly generated broadband ultrafast laser have been increasingly utilized in many applications. However, traditional techniques of characterizing these sources lack the ability to observe the instantaneous features and transitory behaviours of both amplitude and phase. With the advent of the optical time stretch techniques, the instantaneous shotto- shot spectral intensity can be directly measured continuously at an unprecedentedly high speed. Meanwhile, the information of the real-time phase variation, which is carried by the frequency-time mapped spectral signal has yet been fully explored. We present a technique of experimentally measuring the spectral coherence dynamics of broadband pulsed sources. Our method relies on a delayed Young's type interferometer combined with optical time-stretch. We perform the proof-of-principle demonstrations of spectral coherence dynamics measurement on two sources: a supercontinuum source and a fiber ring buffered cavity source, both with a repetition rate of MHz. By employing the optical time stretch with a dispersive fiber, we directly map the spectral interference fringes of the delayed neighbouring pulses and obtain a sufficiently large ensemble of spectral interferograms with a real-time oscilloscope (80Gb/s sampling rate). This enables us to directly quantify the spectral coherence dynamics of the ultrafast sources with a temporal resolution down to microseconds. Having the ensemble of single-shot interferograms, we also further calculate the cross spectral coherence correlation matrices of these ultrafast sources. We anticipate that our technique provides a general approach for experimentally evaluating the spectral coherence dynamics of ultrafast laser generated by the nonlinear processes e.g. modulation instability, supercontinuum generation, and Kerr resonator.

  4. Real-time simultaneous temperature and strain measurements at cryogenic temperatures in an optical fiber

    NASA Astrophysics Data System (ADS)

    Mahar, Scott; Geng, Jihong; Schultz, Joel; Minervini, Joseph; Jiang, Shibin; Titus, Peter; Takayasu, Makoto; Gung, Chen-yu; Tian, Wenyan; Chavez-Pirson, Arturo

    2008-08-01

    A novel fiber optic sensor has been developed to be used in superconducting magnets for fusion reactors and other large cable-in-conduit superconductor (CICC) magnet applications. These large superconducting magnets need a diagnostic that can measure the temperature and strain throughout the magnet in real-time, which was not possible until now. Simultaneous temperature and strain measurements at cryogenic temperatures have been demonstrated, using spontaneous Brillouin scattering in an optical fiber. Using an extremely narrow (100 Hz) linewidth Brillouin laser with very low noise as a frequency shifted local oscillator, the frequency shift of spontaneous Brillouin scattered light was measured using heterodyne detection. A pulsed laser was used to probe the fiber using Optical Time Domain Reflectometry (OTDR) to determine spatial resolution. The spontaneous Brillouin frequency shift and linewidth as a function of temperature agree with previous literature on stimulated Brillouin scattering data from room temperature down to 4 K. For the first time, the spontaneous Brillouin frequency shift, linewidth, and intensity as a function of strain have been measured down to 4 K. Analyzing the frequency spectrum of the scattered light after an FFT gives the Brillouin frequency shift, linewidth, and intensity of the scattered light. 65,000 pulses, with 53 ns pulse widths, were averaged in under one second, providing a 5 meter spatial resolution along a fiber that was about 100 m long. Measuring these three parameters allow the simultaneous determination of temperature and strain in real-time throughout a fiber with a spatial resolution on the order of several meters.

  5. Real-time optical flow estimation on a GPU for a skied-steered mobile robot

    NASA Astrophysics Data System (ADS)

    Kniaz, V. V.

    2016-04-01

    Accurate egomotion estimation is required for mobile robot navigation. Often the egomotion is estimated using optical flow algorithms. For an accurate estimation of optical flow most of modern algorithms require high memory resources and processor speed. However simple single-board computers that control the motion of the robot usually do not provide such resources. On the other hand, most of modern single-board computers are equipped with an embedded GPU that could be used in parallel with a CPU to improve the performance of the optical flow estimation algorithm. This paper presents a new Z-flow algorithm for efficient computation of an optical flow using an embedded GPU. The algorithm is based on the phase correlation optical flow estimation and provide a real-time performance on a low cost embedded GPU. The layered optical flow model is used. Layer segmentation is performed using graph-cut algorithm with a time derivative based energy function. Such approach makes the algorithm both fast and robust in low light and low texture conditions. The algorithm implementation for a Raspberry Pi Model B computer is discussed. For evaluation of the algorithm the computer was mounted on a Hercules mobile skied-steered robot equipped with a monocular camera. The evaluation was performed using a hardware-in-the-loop simulation and experiments with Hercules mobile robot. Also the algorithm was evaluated using KITTY Optical Flow 2015 dataset. The resulting endpoint error of the optical flow calculated with the developed algorithm was low enough for navigation of the robot along the desired trajectory.

  6. Real-time three-dimensional Fourier-domain optical coherence tomography video image guided microsurgeries

    NASA Astrophysics Data System (ADS)

    Kang, Jin U.; Huang, Yong; Zhang, Kang; Ibrahim, Zuhaib; Cha, Jaepyeong; Lee, W. P. Andrew; Brandacher, Gerald; Gehlbach, Peter L.

    2012-08-01

    The authors describe the development of an ultrafast three-dimensional (3D) optical coherence tomography (OCT) imaging system that provides real-time intraoperative video images of the surgical site to assist surgeons during microsurgical procedures. This system is based on a full-range complex conjugate free Fourier-domain OCT (FD-OCT). The system was built in a CPU-GPU heterogeneous computing architecture capable of video OCT image processing. The system displays at a maximum speed of 10 volume/s for an image volume size of 160×80×1024 (X×Y×Z) pixels. We have used this system to visualize and guide two prototypical microsurgical maneuvers: microvascular anastomosis of the rat femoral artery and ultramicrovascular isolation of the retinal arterioles of the bovine retina. Our preliminary experiments using 3D-OCT-guided microvascular anastomosis showed optimal visualization of the rat femoral artery (diameter<0.8 mm), instruments, and suture material. Real-time intraoperative guidance helped facilitate precise suture placement due to optimized views of the vessel wall during anastomosis. Using the bovine retina as a model system, we have performed "ultra microvascular" feasibility studies by guiding handheld surgical micro-instruments to isolate retinal arterioles (diameter˜0.1 mm). Isolation of the microvessels was confirmed by successfully passing a suture beneath the vessel in the 3D imaging environment.

  7. Real-time Functional Analysis of Inertial Microfluidic Devices via Spectral Domain Optical Coherence Tomography

    PubMed Central

    Dong, Biqin; Chen, Siyu; Zhou, Fan; Chan, Christina H. Y.; Yi, Ji; Zhang, Hao F.; Sun, Cheng

    2016-01-01

    We report the application of spectral-domain optical coherence tomography (SD-OCT) technology that enables real-time functional analysis of sorting microparticles and cells in an inertial microfluidic device. We demonstrated high-speed, high-resolution acquisition of cross-sectional images at a frame rate of 350 Hz, with a lateral resolution of 3 μm and an axial resolution of 1 μm within the microfluidic channel filled with water. We analyzed the temporal sequence of cross-sectional SD-OCT images to determine the position and diameter of microspheres in a spiral microfluidic channel under various flow rates. We used microspheres with known diameters to validate the sub-micrometer precision of the particle size analysis based on a scattering model of spherical microparticles. An additional investigation of sorting live HT-29 cells in the spiral microfluidic channel indicated that the distribution of cells within in the microchannel has a close correspondence with the cells’ size distribution. The label-free real-time imaging and analysis of microscale particles in flow offers robustness for practical applications with live cells and allows us to better understand the mechanisms of particle separations in microfluidic sorting systems. PMID:27619202

  8. Real-time, closed-loop dual-wavelength optical polarimetry for glucose monitoring

    PubMed Central

    Malik, Bilal H.; Coté, Gerard L.

    2010-01-01

    The development of a real-time, dual-wavelength optical polarimetric system to ultimately probe the aqueous humor glucose concentrations as a means of noninvasive diabetic glucose monitoring is the long-term goal of this research. The key impact of the work is the development of an approach for the reduction of the time-variant corneal birefringence due to motion artifact, which is still a limiting factor preventing the realization of such a device. Our dual-wavelength approach utilizes real-time, closed-loop feedback that employs a classical three-term feedback controller and efficiently reduces the effect of motion artifact that appears as a common noise source for both wavelengths. In vitro results are shown for the open-loop system, and although the dual-wavelength system helps to reduce the noise, it is shown that closed-loop control is necessary to bring the noise down to a sufficient level for physiological monitoring. Specifically, in vitro measurement results with the closed-loop dual-wavelength approach demonstrate a sensitivity of 12.8 mg∕dl across the physiologic glucose range in the presence of time-variant test cell birefringence. Overall, it is shown that this polarimetric system has the potential to be used as a noninvasive measure of glucose for diabetes. PMID:20210476

  9. Intraoperative brain hemodynamic response assessment with real-time hyperspectral optical imaging (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Laurence, Audrey; Pichette, Julien; Angulo-Rodríguez, Leticia M.; Saint Pierre, Catherine; Lesage, Frédéric; Bouthillier, Alain; Nguyen, Dang Khoa; Leblond, Frédéric

    2016-03-01

    Following normal neuronal activity, there is an increase in cerebral blood flow and cerebral blood volume to provide oxygenated hemoglobin to active neurons. For abnormal activity such as epileptiform discharges, this hemodynamic response may be inadequate to meet the high metabolic demands. To verify this hypothesis, we developed a novel hyperspectral imaging system able to monitor real-time cortical hemodynamic changes during brain surgery. The imaging system is directly integrated into a surgical microscope, using the white-light source for illumination. A snapshot hyperspectral camera is used for detection (4x4 mosaic filter array detecting 16 wavelengths simultaneously). We present calibration experiments where phantoms made of intralipid and food dyes were imaged. Relative concentrations of three dyes were recovered at a video rate of 30 frames per second. We also present hyperspectral recordings during brain surgery of epileptic patients with concurrent electrocorticography recordings. Relative concentration maps of oxygenated and deoxygenated hemoglobin were extracted from the data, allowing real-time studies of hemodynamic changes with a good spatial resolution. Finally, we present preliminary results on phantoms obtained with an integrated spatial frequency domain imaging system to recover tissue optical properties. This additional module, used together with the hyperspectral imaging system, will allow quantification of hemoglobin concentrations maps. Our hyperspectral imaging system offers a new tool to analyze hemodynamic changes, especially in the case of epileptiform discharges. It also offers an opportunity to study brain connectivity by analyzing correlations between hemodynamic responses of different tissue regions.

  10. Real-time three-dimensional Fourier-domain optical coherence tomography video image guided microsurgeries

    PubMed Central

    Huang, Yong; Zhang, Kang; Ibrahim, Zuhaib; Cha, Jaepyeong; Lee, W. P. Andrew; Brandacher, Gerald; Gehlbach, Peter L.

    2012-01-01

    Abstract. The authors describe the development of an ultrafast three-dimensional (3D) optical coherence tomography (OCT) imaging system that provides real-time intraoperative video images of the surgical site to assist surgeons during microsurgical procedures. This system is based on a full-range complex conjugate free Fourier-domain OCT (FD-OCT). The system was built in a CPU-GPU heterogeneous computing architecture capable of video OCT image processing. The system displays at a maximum speed of 10  volume/s for an image volume size of 160×80×1024 (X×Y×Z) pixels. We have used this system to visualize and guide two prototypical microsurgical maneuvers: microvascular anastomosis of the rat femoral artery and ultramicrovascular isolation of the retinal arterioles of the bovine retina. Our preliminary experiments using 3D-OCT-guided microvascular anastomosis showed optimal visualization of the rat femoral artery (diameter<0.8  mm), instruments, and suture material. Real-time intraoperative guidance helped facilitate precise suture placement due to optimized views of the vessel wall during anastomosis. Using the bovine retina as a model system, we have performed “ultra microvascular” feasibility studies by guiding handheld surgical micro-instruments to isolate retinal arterioles (diameter∼0.1  mm). Isolation of the microvessels was confirmed by successfully passing a suture beneath the vessel in the 3D imaging environment. PMID:23224164

  11. Real-Time Optical Surveillance of LEO/MEO with Small Telescopes

    NASA Astrophysics Data System (ADS)

    Zimmer, P.; McGraw, J.; Ackermann, M.

    J.T. McGraw and Associates, LLC operates two proof-of-concept wide-field imaging systems to test novel techniques for uncued surveillance of LEO/MEO/GEO and, in collaboration with the University of New Mexico (UNM), uses a third small telescope for rapidly queued same-orbit follow-up observations. Using our GPU-accelerated detection scheme, the proof-of-concept systems operating at sites near and within Albuquerque, NM, have detected objects fainter than V=13 at greater than 6 sigma significance. This detection approximately corresponds to a 16 cm object with albedo of 0.12 at 1000 km altitude. Dozens of objects are measured during each operational twilight period, many of which have no corresponding catalog object. The two proof-of-concept systems, separated by ~30km, work together by taking simultaneous images of the same orbital volume to constrain the orbits of detected objects using parallax measurements. These detections are followed-up by imaging photometric observations taken at UNM to confirm and further constrain the initial orbit determination and independently assess the objects and verify the quality of the derived orbits. This work continues to demonstrate that scalable optical systems designed for real-time detection of fast moving objects, which can be then handed off to other instruments capable of tracking and characterizing them, can provide valuable real-time surveillance data at LEO and beyond, which substantively informs the SSA process.

  12. Real-time dosimetry with Yb-doped silica optical fibres.

    PubMed

    Veronese, Ivan; Chiodini, Norberto; Cialdi, Simone; D'Ippolito, Eduardo; Fasoli, Mauro; Gallo, Salvatore; La Torre, Stefano; Mones, Eleonora; Vedda, Anna; Loi, Gianfranco

    2017-03-02

    Over the years, many efforts have been done to develop radiation detectors to afford the complex issues of small field dosimetry and to fulfil the needs of increasing accuracy, precision and in-vivo dose monitoring required by the new advanced treatment modalities. In this context, a growing interest has surged in the development of sensors based on scintillating optical fibres. In this paper, the near-infrared radioluminescence and dosimetric properties of Yb-doped silica optical fibres, coupled with a laboratory prototype based on an avalanche photo-diode, were studied by irradiating the fibres with photons and electron beams generated by a Varian Trilogy accelerator. The performances of the system in standard and small field sizes have been also investigated comparing the output factor, percent depth dose and off axis ratio measurements of the prototypal detector with other commercial sensors, including the Exradin W1 scintillator. The results of this study demonstrated that the drawback due to the stem effect in Yb-doped silica optical fibres can be managed in a simple but effective way by optical filtering. The robustness of the system in complex dosimetric scenarios and the accuracy and the precision achieved by Yb-doped fibres in relative dose assessments suggest an effective use of the system for real time in-vivo dosimetry applications.

  13. Real time dynamic strain monitoring of optical links using the backreflection of live PSK data.

    PubMed

    Martins, H F; Shi, K; Thomsen, B C; Martin-Lopez, S; Gonzalez-Herraez, M; Savory, S J

    2016-09-19

    A major cause of faults in optical communication links is related to unintentional third party intrusions (normally related to civil/agricultural works) causing fiber breaks or cable damage. These intrusions could be anticipated and avoided by monitoring the dynamic strain recorded along the cable. In this work, a novel technique is proposed to implement real-time distributed strain sensing in parallel with an operating optical communication channel. The technique relies on monitoring the Rayleigh backscattered light from optical communication data transmitted using standard modulation formats. The system is treated as a phase-sensitive OTDR (ΦOTDR) using random and non-periodical non-return-to-zero (NRZ) phase-shift keying (PSK) pulse coding. An I/Q detection unit allows for a full (amplitude, phase and polarization) characterization of the backscattered optical signal, thus achieving a fully linear system in terms of ΦOTDR trace coding/decoding. The technique can be used with different modulation formats, and operation using 4 Gbaud single-polarization dual PSK and 4 Gbaud dual-polarization quadrature PSK is demonstrated. As a proof of concept, distributed sensing of dynamic strain with a sampling of 125 kHz and a spatial resolution of 2.5 cm (set by the bit size) over 500 m is demonstrated for applied sinusoidal strain signals of 500 Hz. The limitations and possibilities for improvement of the technique are also discussed.

  14. Real-Time Optical Fuel-to-Air Ratio Sensor for Gas Turbine Combustors

    NASA Technical Reports Server (NTRS)

    Nguyen, Quang-Viet; Mongia, Rajiv K.; Dibble, Robert W.

    1999-01-01

    The measurement of the temporal distribution of fuel in gas turbine combustors is important in considering pollution, combustion efficiency and combustor dynamics and acoustics. Much of the previous work in measuring fuel distributions in gas turbine combustors has focused on the spatial aspect of the distribution. The temporal aspect however, has often been overlooked, even though it is just as important. In part, this is due to the challenges of applying real-time diagnostic techniques in a high pressure and high temperature environment. A simple and low-cost instrument that non-intrusively measures the real-time fuel-to-air ratio (FAR) in a gas turbine combustor has been developed. The device uses a dual wavelength laser absorption technique to measure the concentration of most hydrocarbon fuels such as jet fuel, methane, propane, etc. The device can be configured to use fiber optics to measure the local FAR inside a high pressure test rig without the need for windows. Alternatively, the device can readily be used in test rigs that have existing windows without modifications. An initial application of this instrument was to obtain time-resolved measurements of the FAR in the premixer of a lean premixed prevaporized (LPP) combustor at inlet air pressures and temperatures as high as 17 atm at 800 K, with liquid JP-8 as the fuel. Results will be presented that quantitatively show the transient nature of the local FAR inside a LPP gas turbine combustor at actual operating conditions. The high speed (kHz) time resolution of this device, combined with a rugged fiber optic delivery system, should enable the realization of a flight capable active-feedback and control system for the abatement of noise and pollutant emissions in the future. Other applications that require an in-situ and time-resolved measurement of fuel vapor concentrations should also find this device to be of use.

  15. Real time optical coherence tomography monitoring of Candida albicans biofilm in vitro during photodynamic treatment

    NASA Astrophysics Data System (ADS)

    Suzuki, Luis Cláudio; Araujo Prates, Renato; Raele, Marcus Paulo; Zanardi di Freitas, Anderson; Simões Ribeiro, Martha

    2010-04-01

    The biofilm formed by Candida albicans is the mainly cause of infections associated to medical devices such as catheters. Studies have shown that photodynamic antimicrobial therapy (PAT) has lethal effect on C. albicans, and it is based on photosensitizer (PS) in the presence of low intensity light to generate reactive oxygen species in biological systems. The aim of this study was to analyze in real time, by Optical Coherence Tomography (OCT), the alterations in C. albicans biofilm in vitro during PAT using methylene blue (MB) as a PS and red light. An OCT system with working at 930nm was used, sequential images of 2000×512 pixels were generated at the frame rate of 2.5frames/sec. The dimension of the analyzed sample was 6000μm wide by 1170μm of depth corrected by refraction index of 1.35. We recorded 1min. before and after the irradiation with LED for PAT, generating 8min. of video. For biofilm formation, discs were made from elastomeric silicone catheters. The PS was dissolved in PBS solution, and a final concentration of 1mM MB was applied on biofilm, followed by a red LED irradiation (λ=630nm+/-20nm) during 6min. We performed a curve of survival fraction versus time of irradiation and it was reduced by 100% following 6min. of irradiation. OCT was performed for measurement of biofilm thickness of 110μm when biofilm was formed. During irradiation, the variation of biofilm thickness was ~70μm. We conclude that OCT system is able to show real time optical changes provided by PAT in yeasts organized in biofilm.

  16. Real-time optical monitoring of permanent lesion progression in radiofrequency ablated cardiac tissue (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Singh-Moon, Rajinder P.; Hendon, Christine P.

    2016-02-01

    Despite considerable advances in guidance of radiofrequency ablation (RFA) therapies for atrial fibrillation, success rates have been hampered by an inability to intraoperatively characterize the extent of permanent injury. Insufficient lesions can elusively create transient conduction blockages that eventually reconduct. Prior studies suggest significantly greater met-myoglobin (Mmb) concentrations in the lesion core than those in the healthy myocardium and may serve as a marker for irreversible tissue damage. In this work, we present real-time monitoring of permanent injury through spectroscopic assessment of Mmb concentrations at the catheter tip. Atrial wedges (n=6) were excised from four fresh swine hearts and submerged under pulsatile flow of warm (37oC) phosphate buffered saline. A commercial RFA catheter inserted into a fiber optic sheath allowed for simultaneous measurement of tissue diffuse reflectance (DR) spectra (500-650nm) during application of RF energy. Optical measurements were continuously acquired before, during, and post-ablation, in addition to healthy neighboring tissue. Met-myoglobin, oxy-myoglobin, and deoxy-myoglobin concentrations were extracted from each spectrum using an inverse Monte Carlo method. Tissue injury was validated with Masson's trichrome and hematoxylin and eosin staining. Time courses revealed a rapid increase in tissue Mmb concentrations at the onset of RFA treatment and a gradual plateauing thereafter. Extracted Mmb concentrations were significantly greater post-ablation (p<0.0001) as compared to healthy tissue and correlated well with histological assessment of severe thermal tissue destruction. On going studies are aimed at integrating these findings with prior work on near infrared spectroscopic lesion depth assessment. These results support the use of spectroscopy-facilitated guidance of RFA therapies for real-time permanent injury estimation.

  17. Germanium-doped optical fiber for real-time radiation dosimetry

    NASA Astrophysics Data System (ADS)

    Mizanur Rahman, A. K. M.; Zubair, H. T.; Begum, Mahfuza; Abdul-Rashid, H. A.; Yusoff, Z.; Ung, N. M.; Mat-Sharif, K. A.; Wan Abdullah, W. S.; Amouzad Mahdiraji, Ghafour; Amin, Y. M.; Maah, M. J.; Bradley, D. A.

    2015-11-01

    Over the past three decades growing demand for individualized in vivo dosimetry and subsequent dose verification has led to the pursuit of newer, novel and economically feasible materials for dosimeters. These materials are to facilitate features such as real-time sensing and fast readouts. In this paper, purposely composed SiO2:Ge optical fiber is presented as a suitable candidate for dosimetry. The optical fiber is meant to take advantage of the RL/OSL technique, providing both online remote monitoring of dose rate, and fast readouts for absorbed dose. A laboratory-assembled OSL reader has been used to acquire the RL/OSL response to LINAC irradiations (6 MV photons). The notable RL characteristics observed include constant level of luminescence for the same dose rate (providing better consistency compared to TLD-500), and linearity of response in the radiotherapy range (1 Gy/min to 6 Gy/min). The OSL curve was found to conform to an exponential decay characteristic (illumination with low LED source). The Ge doping resulted in an effective atomic number, Zeff, of 13.5 (within the bone equivalent range). The SiO2:Ge optical fiber sensor, with efficient coupling, can be a viable solution for in vivo dosimetry, besides a broad range of applications.

  18. Development of a real time MTF test bench for visible optical systems

    NASA Astrophysics Data System (ADS)

    Chen, Xinhua; Chen, Yuheng; Fan, Jiming; Xiang, Chunchang; Shen, Weimin

    2010-11-01

    A real-time MTF test bench for visible optical systems is presented in this paper. This test bench can perform quick on-axis and off-axis MTF measurement of optical systems whose aperture are less than 200mm in visible wavelength. A high quality off-axis parabolic collimator is used as object generator of this test bench. The image analyzer is a microscopy with CCD camera installed on a multi-axis motion stage. The software of this MTF test bench provides a good interface for the operators to set measurement parameters and control this bench. Validation of this test bench, performed with a 50mm plano-convex audit lens, shows that MTF measurement error of this bench is within 0.04. Besides MTF measurement, this bench can also perform effective focal length (EFL) and back focal length (BFL) without any hardware modification. Transmittance of optical system can also be performed on this bench with an integrating sphere.

  19. Compact remote optical system for real time measurement of aerosol emissions

    NASA Astrophysics Data System (ADS)

    Waldherr, Gregor A.; Wylie, Michael T. V.; Lin, Hai

    2014-05-01

    Demonstrating particle matter (PM) emissions compliance with environmental regulations is required for operation of any industrial, commercial or military facility. Emission sources are initially screened based on their visible opacity via US EPA Method 9 standard. Nowadays, it is rather surprising that opacity is still visually determined by human eyes with smoke school training and semi-annual certification. Nonetheless, opacity alone doesn't reveal particle concentration and size distribution which is crucial to determine PM values. It would be desirable to have an automated, calibratable device to make such a measurement with higher accuracy and reliability and is not available to date. We present our preliminary work on developing a compact, hand portable system capable of quantitatively determining plume optical opacity in combination with possible particle concentration and size distribution (and hence PM) measurement for standoff distances up to 150 meters. Our benchtop lidar-based system was built with a frequencydoubled Q-switched Nd:YAG laser and an optical receiver. We have demonstrated the feasibility of a remote-capable optical sensor system for real-time continuous monitoring of visible emission plumes and its effectiveness for quantitative opacity measurement. The hand-portable Remote Plume Particulate Matter (RPPM) meter being developed will be capable of determining opacity and PM load from remote PM sources will provide a regulatory compliance solution for visible emissions from sources for environmental studies, industrial and military applications.

  20. Feasibility of a compact fiber optic probe for real time tracing of subsurface skin birefringence

    NASA Astrophysics Data System (ADS)

    Tugbaev, Vitaly; Myllyla, Risto

    2005-08-01

    A novel approach anticipates real time acquisition of spatially resolved polarization data to facilitate fast cross-sectional tracing collagen-related birefringence in skin down to reticular dermis, i.e. up to the depth of a few hundreds micrometers. It is based on a unique integration of a static-type interferometer in a time domain system intended for polarization-sensitive optical coherence tomography (PS-OCT). The design concept avoids any movable parts to evolve fringes over the traced depth, and exploits liquid crystal bistable switches to rapidly discriminate between orthogonal polarization components of the analyzable signal. The signal is transmitted through a polarization maintaining fiber and detected, by turns, in the single optical channel by the same line camera of appropriate format. The approach relies on the statements proven in the art. In particular, time-domain PS-OCT based on coherent detection of the fringe intensity in orthogonal polarization components of reflected signal allows identifying at least qualitatively collagen depletion regions in subsurface skin layers. Polarization state of light backscattered from sufficiently shallow depth in skin is defined mostly by linear birefringence of collagen fibers. Propagation of light in such linearly birefringent medium satisfies the reciprocity principle in optics.

  1. Portable real-time optical coherence tomography system for intraoperative imaging and staging of breast cancer

    NASA Astrophysics Data System (ADS)

    Nguyen, Freddy T.; Zysk, Adam M.; Kotynek, Jan G.; Bellafiore, Frank J.; Rowland, Kendrith M.; Johnson, Patricia A.; Chaney, J. Eric; Boppart, Stephen A.

    2007-02-01

    Breast cancer continues to be one of the most widely diagnosed forms of cancer amongst women and the second leading type of cancer deaths amongst women. The recurrence rate of breast cancer is highly dependent on several factors including the complete removal of the primary tumor and the presence of cancer cells in involved lymph nodes. The metastatic spread and staging of breast cancer is also evaluated through the nodal assessment of the regional lymphatic system. A portable real-time spectral domain optical coherence tomography system is being presented as a clinical diagnostic tool in the intraoperative delineation of tumor margins as well as for real time lymph node assessment. The system employs a super luminescent diode centered at 1310 nm with a bandwidth of 92 nm. Using a spectral domain detection system, the data is acquired at a rate of 5 KHz / axial scan. The sample arm is a galvanometer scanning telecentric probe with an objective lens (f = 60 mm, confocal parameter = 1.5 mm) yielding an axial resolution of 8.3 μm and a transverse resolution of 35.0 μm. Images of tumor margins are acquired in the operating room ex vivo on freshly excised human tissue specimen. This data shows the potential of the use of OCT in defining the structural tumor margins in breast cancer. Images taken from ex-vivo samples on the bench system clearly delineate the differences between clusters of tumor cells and nearby adipose cells. In addition, the data shows the potential for OCT as a diagnostic tool in the staging of cancer metastasis through locoregional lymph node assessment.

  2. Integrating SAR with Optical and Thermal Remote Sensing for Operational Near Real-Time Volcano Monitoring

    NASA Astrophysics Data System (ADS)

    Meyer, F. J.; Webley, P.; Dehn, J.; Arko, S. A.; McAlpin, D. B.

    2013-12-01

    multiple observation geometries in change detection procedures. Additionally, it will be shown how SAR-based hazard information can be integrated with data from optical satellites, thermal sensors, webcams and models to create near-real time volcano hazard information. We will introduce a prototype monitoring system that integrates SAR-based hazard information into the near real-time volcano hazard monitoring system of the Alaska Volcano Observatory. This prototype system was applied to historic eruptions of the volcanoes Okmok and Augustine, both located in the North Pacific. We will show that for these historic eruptions, the addition of SAR data lead to a significant improvement in activity detection and eruption monitoring, and improved the accuracy and timeliness of eruption alerts.

  3. 16-QAM optical packet switching and real-time self-homodyne detection using polarization-multiplexed pilot-carrier.

    PubMed

    Shinada, Satoshi; Nakamura, Moriya; Kamio, Yukiyoshi; Wada, Naoya

    2012-12-10

    We demonstrated 20-Gbit/s 16 quadrature amplitude modulation (16-QAM) optical packet switching and real-time detection using self-homodyne. A prototype modulator consisting of an in-phase and quadrature (I-Q) modulator and monolithically integrated polarization beam splitters generated modulated signals and polarization-multiplexed pilot-carriers simultaneously. Self-homodyne detection using the pilot-carrier was resilient to phase noise and self-phase modulation, and the constellation was obtained in real time without digital signal processing. A low-polarization-dependent (Pb,La)(Zr,La)O(3) (PLZT) optical switch in the optical packet switch handled both 16-QAM optical packets and the polarization multiplexed pilot-carrier. Even after packet switching, a clear constellation diagram was obtained, and error-free operation was confirmed in real-time using a packet bit-error rate tester (BERT).

  4. Real-time determination of telomerase activity in cell extracts using an optical biosensor.

    PubMed

    Schmidt, Peter M; Matthes, Eckart; Scheller, Frieder W; Bienert, Michael; Lehmann, Christine; Ehrlich, Angelika; Bier, Frank F

    2002-10-01

    A biosensoric approach has been developed to determine the activity of telomerase in tumor cell lysates. An optical sensor, the grating coupler, was used to monitor the association and dissociation of unlabeled compounds on the sensor surface in real time, by virtue of an evanescent field. An oligonucleotide was immobilized on the surface of the optical biosensor and linked with two other oligonucleotides by complementary sequences in an overlapping manner. The 3'-end of the last one carried the sequence of the telomeric substrate (TS) primer used for elongation by telomerase in the telomeric repeat amplification protocol (TRAP) assay. This primer sequence was phosphorothioate (PS)-modified, which is known to strongly increase the affinity to the primer binding site of telomerase protein and consequently the velocity of the telomerase reaction. We show that the PS primer binds to the modified biosensor and is elongated effectively by the telomerase from HL-60 cell lysates. A synthesis rate of 1 nucleotide/min was determined. The inhibitory effect of peptide nucleic acid (PNA) was shown by using immobilized TS. The velocity of the telomerase reaction was slowed down and the signal intensity was below the signal-to-noise ratio. Most nucleic acid detection systems use amplification steps such as polymerase chain reaction (PCR) to increase the amount of the probe. Since telomerase is a polymerase itself amplification of DNA by PCR is not required. Furthermore, no purification steps were required since all measurements were performed with crude cell extract.

  5. Optical coherence tomography imaging of retinal damage in real time under a stimulus electrode

    NASA Astrophysics Data System (ADS)

    Cohen, Ethan; Agrawal, Anant; Connors, Megan; Hansen, Barry; Charkhkar, Hamid; Pfefer, Joshua

    2011-10-01

    We have developed a novel method to study the effects of electrical stimulation of the local retina directly under an epiretinal stimulus electrode in real time. Using optical coherence tomography (OCT) and a superfused retinal eyecup preparation, we obtained high-resolution images of the rabbit retina directly under an optically transparent saline-filled fluoropolymer stimulation tube electrode. During OCT imaging, 50 Hz trains of biphasic current pulses 1 ms/phase (23-749 µC cm-2 ph-1) were applied to the retinal surface for 5 min. After imaging, the stimulated regions were stained with the dye propidium iodide (PI) to reveal cytotoxic damage. Pulse train stimulation at 44-133 µC cm-2 ph-1 had little effect on the retina; however, trains >=442 µC cm-2 ph-1 caused increases in the reflectance of the inner plexiform layer (IPL) and edema. The damage seen in retinal OCT images matched the pattern observed in histological sections, and in the PI staining. With pulse trains >=442 µC cm-2 ph-1, rapid increases in the reflectivity of the IPL could be observed under the stimulus electrode. Below the electrode, we observed a ring-like pattern of retinal detachment in the subretinal space. The OCT imaging method may be useful for analyzing overstimulation of neuronal tissue by electrodes in many brain regions. This paper was originally submitted for the special issue containing contributions from the Sixth Biennial Research Congress of The Eye and the Chip.

  6. Near Real-Time Automatic Marine Vessel Detection on Optical Satellite Images

    NASA Astrophysics Data System (ADS)

    Máttyus, G.

    2013-05-01

    Vessel monitoring and surveillance is important for maritime safety and security, environment protection and border control. Ship monitoring systems based on Synthetic-aperture Radar (SAR) satellite images are operational. On SAR images the ships made of metal with sharp edges appear as bright dots and edges, therefore they can be well distinguished from the water. Since the radar is independent from the sun light and can acquire images also by cloudy weather and rain, it provides a reliable service. Vessel detection from spaceborne optical images (VDSOI) can extend the SAR based systems by providing more frequent revisit times and overcoming some drawbacks of the SAR images (e.g. lower spatial resolution, difficult human interpretation). Optical satellite images (OSI) can have a higher spatial resolution thus enabling the detection of smaller vessels and enhancing the vessel type classification. The human interpretation of an optical image is also easier than as of SAR image. In this paper I present a rapid automatic vessel detection method which uses pattern recognition methods, originally developed in the computer vision field. In the first step I train a binary classifier from image samples of vessels and background. The classifier uses simple features which can be calculated very fast. For the detection the classifier is slided along the image in various directions and scales. The detector has a cascade structure which rejects most of the background in the early stages which leads to faster execution. The detections are grouped together to avoid multiple detections. Finally the position, size(i.e. length and width) and heading of the vessels is extracted from the contours of the vessel. The presented method is parallelized, thus it runs fast (in minutes for 16000 × 16000 pixels image) on a multicore computer, enabling near real-time applications, e.g. one hour from image acquisition to end user.

  7. Applications of Non-Crystalline Materials — C. REAL TIME OPTICAL RECORDING ON THIN FILMS OF AMORPHOUS SEMICONDUCTORS

    NASA Astrophysics Data System (ADS)

    Mitkova, Maria

    The following sections are included: * Introduction * Amorphous Semiconductors as Optical Storage Medium * Principles and Results on Real-time Optical Recording * Digital optical recording * Digital optical recording due to ablation of the films by illumination with light * Digital optical recording due to coagulation of an island film * Digital optical recording due to phase transition * Digital optical recording due to photoinduced surface deposition of metallic silver * Principles of holographic recording * Recording and readout * Classification of the holograms * Diffraction efficiency * Results in holographic recording on chalcogenide glasses * Future Trends * Acknowledgments * References

  8. Real time imaging of peripheral nerve vasculature using optical coherence angiography

    NASA Astrophysics Data System (ADS)

    Vasudevan, Srikanth; Kumsa, Doe; Takmakov, Pavel; Welle, Cristin G.; Hammer, Daniel X.

    2016-03-01

    The peripheral nervous system (PNS) carries bidirectional information between the central nervous system and distal organs. PNS stimulation has been widely used in medical devices for therapeutic indications, such as bladder control and seizure cessation. Investigational uses of PNS stimulation include providing sensory feedback for improved control of prosthetic limbs. While nerve safety has been well documented for stimulation parameters used in marketed devices, novel PNS stimulation devices may require alternative stimulation paradigms to achieve maximum therapeutic benefit. Improved testing paradigms to assess the safety of stimulation will expedite the development process for novel PNS stimulation devices. The objective of this research is to assess peripheral nerve vascular changes in real-time with optical coherence angiography (OCA). A 1300-nm OCA system was used to image vasculature changes in the rat sciatic nerve in the region around a surface contacting single electrode. Nerves and vasculature were imaged without stimulation for 180 minutes to quantify resting blood vessel diameter. Walking track analysis was used to assess motor function before and 6 days following experiments. There was no significant change in vessel diameter between baseline and other time points in all animals. Motor function tests indicated the experiments did not impair functionality. We also evaluated the capabilities to image the nerve during electrical stimulation in a pilot study. Combining OCA with established nerve assessment methods can be used to study the effects of electrical stimulation safety on neural and vascular tissue in the periphery.

  9. Optical fingerprinting in bacterial epidemiology: Raman spectroscopy as a real-time typing method.

    PubMed

    Willemse-Erix, Diana F M; Scholtes-Timmerman, Maarten J; Jachtenberg, Jan-Willem; van Leeuwen, Willem B; Horst-Kreft, Deborah; Bakker Schut, Tom C; Deurenberg, Ruud H; Puppels, Gerwin J; van Belkum, Alex; Vos, Margreet C; Maquelin, Kees

    2009-03-01

    Hospital-acquired infections (HAI) increase morbidity and mortality and constitute a high financial burden on health care systems. An effective weapon against HAI is early detection of potential outbreaks and sources of contamination. Such monitoring requires microbial typing with sufficient reproducibility and discriminatory power. Here, a microbial-typing method is presented, based on Raman spectroscopy. This technique provides strain-specific optical fingerprints in a few minutes instead of several hours to days, as is the case with genotyping methods. Although the method is generally applicable, we used 118 Staphylococcus aureus isolates to illustrate that the discriminatory power matches that of established genotyping techniques (numerical index of diversity [D]=0.989) and that concordance with the gold standard (pulsed-field gel electrophoresis) is high (95%). The Raman clustering of isolates was reproducible to the strain level for five independent cultures, despite the various culture times from 18 h to 24 h. Furthermore, this technique was able to classify stored (-80 degrees C) and recent isolates of a methicillin-resistant Staphylococcus aureus-colonized individual during surveillance studies and did so days earlier than established genotyping techniques did. Its high throughput and ease of use make it suitable for use in routine diagnostic laboratory settings. This will set the stage for continuous, automated, real-time epidemiological monitoring of bacterial infections in a hospital, which can then be followed by timely corrective action by infection prevention teams.

  10. Real-time monitoring of the optical spectrum in laser welding

    NASA Astrophysics Data System (ADS)

    Mueller, Robert E.; Duley, Walter W.

    1997-03-01

    Laser welding has become a standard manufacturing technique, particularly in industries where weld quality and performance are critical, such as the aerospace, nuclear, medical devices and automotive sectors. In many laser welding applications, flaws are not acceptable in the final product, so every weld must be inspected. Post-process inspection is time-consuming and, if a systematic problem develops, many flawed parts could be produced before the problem is identified and corrected. The preferred approach is therefore to perform in-process inspection as the weld is produced. This paper describes a weld process inspection system based on a compact, computer controlled optical spectrometer, which observes the laser welding plume in real time. From the plume spectrum, one is able to determine the temperature of the weld site and the elements present in the fusion zone. A sudden change in weld temperature may indicate a weld flaw, either from a loss of laser energy coupling and therefore a loss of fusion, or from excess energy input and burn-through. An indication of the elements present in the fusion zone can be used for seam tracking or penetration monitoring when dissimilar materials are being joined in the butt or lap configurations, respectively.

  11. Real-time imaging of suction blistering in human skin using optical coherence tomography

    PubMed Central

    Carvalho, Joana C.O.; Palero, Jonathan A.; Jurna, Martin

    2015-01-01

    Separation of skin epidermis from the dermis by suction blistering has been used with high success rate for autologous skin epidermal grafting in burns, chronic wounds and vitiligo transplantation treatment. Although commercial products that achieve epidermal grafting by suction blistering are presently available, there is still limited knowledge and understanding on the dynamic process of epidermal-dermal separation during suction blistering. In this report we integrated a suction system to an Optical Coherence Tomography (OCT) which allowed for the first time, real-time imaging of the suction blistering process in human skin. We describe in this report the evolution of a suction blister where the growth is modeled with a Boltzmann sigmoid function. We further investigated the relationship between onset and steady-state blister times, blister growth rate, applied suction pressure and applied local skin temperature. Our results show that while the blister time is inversely proportional to the applied suction pressure, the relationship between the blister time and the applied temperature is described by an exponential decay. PMID:26713194

  12. Real-time optical monitoring of microbial growth using optimal combination of light-emitting diodes

    NASA Astrophysics Data System (ADS)

    Kobayashi, Ken-ichi; Yamada, Takeshi; Hiraishi, Akira; Nakauchi, Shigeki

    2012-12-01

    We developed a real-time optical monitoring system consisting of a monochrome complementary metal-oxide semiconductor (CMOS) camera and two light-emitting diodes (LEDs) with a constant temperature incubator for the rapid detection of microbial growth on solid media. As a target organism, we used Alicyclobacillus acidocaldarius, which is an acidophilic thermophilic endospore-forming bacterium able to survive in pasteurization processes and grow in acidic drink products such as apple juice. This bacterium was cultured on agar medium with a redox dye applied to improve detection sensitivity. On the basis of spectroscopic properties of the colony, medium, and LEDs, an optimal combination of two LED illuminations was selected to maximize the contrast between the colony and medium areas. We measured A. acidocaldarius and Escherichia coli at two different dilution levels using these two LEDs. From the results of time-course changes in the number of detected pixels in the detection images, a similar growth rate was estimated amongst the same species of microbes, regardless of the dilution level. This system has the ability to detect a colony of approximately 26 μm in diameter in a detection image, and it can be interpreted that the size corresponds to less than 20 μm diameter in visual inspection.

  13. Real-time frequency-domain fiber optic sensor for intra-arterial blood oxygen measurements

    NASA Astrophysics Data System (ADS)

    Alcala, J. R.; Scott, Ian L.; Parker, Jennifer W.; Atwater, Beauford W.; Yu, Clement; Fischer, Russell; Bellingrath, K.

    1993-05-01

    A real time frequency domain phosphorimeter capable of measuring precise and accurate excited state lifetimes for determining oxygen is described. This frequency domain instrument does not make use of cross correlation techniques traditionally used in frequency domain fluorometers. Instead, the electrical signal from the detector is filtered to contain only the first several harmonics. This filtered signal is then sampled and averaged over a few thousand cycles. The absolute phase and absolute modulation of each sampled harmonic of the excitation and of the luminescence is computed by employing fast Fourier transform algorithms. The phase delay and the modulation ratio is then calculated at each harmonic frequency. A least squares fit is performed in the frequency domain to obtain the lifetimes of discrete exponentials. Oxygen concentrations are computed from these lifetimes. Prototypes based on these techniques were built employing commercially available components. Results from measurements in saline solution and in the arterial blood of dogs show that oxygen concentrations can be determined reproducibly. The system drift is less than 1% in over 100 hours of continuous operation. The performance of fiber optic sensors was evaluated in dogs over a period of 10 hours. The sensors tracked changes in arterial oxygen tension over the course of the experiment without instabilities. The overall response of the system was about 90 seconds. The update time was 3 seconds.

  14. The Maximum Entropy Method for Optical Spectrum Analysis of Real-Time TDDFT

    NASA Astrophysics Data System (ADS)

    Toogoshi, M.; Kano, S. S.; Zempo, Y.

    2015-09-01

    The maximum entropy method (MEM) is one of the key techniques for spectral analysis. The major feature is that spectra in the low frequency part can be described by the short time-series data. Thus, we applied MEM to analyse the spectrum from the time dependent dipole moment obtained from the time-dependent density functional theory (TDDFT) calculation in real time. It is intensively studied for computing optical properties. In the MEM analysis, however, the maximum lag of the autocorrelation is restricted by the total number of time-series data. We proposed that, as an improved MEM analysis, we use the concatenated data set made from the several-times repeated raw data. We have applied this technique to the spectral analysis of the TDDFT dipole moment of ethylene and oligo-fluorene with n = 8. As a result, the higher resolution can be obtained, which is closer to that of FT with practically time-evoluted data as the same total number of time steps. The efficiency and the characteristic feature of this technique are presented in this paper.

  15. Real time pulse width monitor for Intensified Charge Coupled Device (ICCD) electro-optic shutters

    SciTech Connect

    Yates, G.J.

    1996-12-01

    A method is described or controlling and measuring the pulse width of electrical gate pulses used for optical shuttering of image intensifier. The intensifiers are coupled to high frame rate Charge-Coupled-Devices (CCD) or Focus-Projection Scan (FPS) vidicon TV cameras for readout and telemetry of time resolved image sequences. The shutter duration or gate width of individual shutters is measured in real time and encoded in the video frame corresponding to a given shutter interval. The shutter information is updated once catch video frame by strobing new data with each TV camera vertical sync pulse. This circuitry is used in conjunction with commercial video insertion/annotation equipment to provide die shutter width information in alpha numeric text form along with the time resolved video image on a frame-by-frame basis. The measurement technique and circuitry involving a combination of high speed digital counters and analog integrators for measurements in the Ins to 1024 ns range are described. The accuracy obtained is compared with measurements obtained using batch speed DSOs. The measured data are provided in 10-bit Binary (Bi) and four decades of Binary Coded Decimal (BCD) and also displayed on four digit seven segment displays. The control circuitry including digital and analog input means for gate width selection are described. The implementation of both measurement and control circuitry into an Intensified Shuttered CCD (ISCCD) radiometric system for recording fast shuttered images at RS-170 to 4 KHz frame rates is presented.

  16. Next generation optical surface sensing for real-time measurement in radiotherapy.

    PubMed

    Parkhurst, James M; Price, Gareth J; Sharrock, Phil J; Marchant, Tom E; Moore, Christopher J

    2011-01-01

    With the introduction of intensive new treatments such as hypo-fractionation and proton beam therapy, localization of the tumor target volume and tracking of points across the skin entrance surface have become critically important. Optical metrology has been used to monitor the patient's bulk position and motion throughout treatment. However systems have not been capable of high temporal and spatial resolution whole-surface topology measurement. We describe the implementation of such a system based on Fourier profilometry. Its algorithm is split into four separate processing stages, including spatial phase determination: descriptions of each stage are given along with the modifications made to increase performance. The optimized system is capable of processing 23 frames per second (fps), with each frame providing 512 × 512 measured points. The data density, accuracy and performance of the system are an order of magnitude improvement on commercially available clinical systems. We show that this performance permits genuinely real-time measurement of a patient, live during both setup and radiation treatment delivery. It is also fast enough to provide smooth dynamic visualizations of motion at all points on the wrap-around body surface for radiotherapy staff and intuitive, direct feed-back to patients.

  17. Fiber-optic probe for noninvasive real-time determination of tissue optical properties at multiple wavelengths.

    PubMed

    Dam, J S; Pedersen, C B; Dalgaard, T; Fabricius, P E; Aruna, P; Andersson-Engels, S

    2001-03-01

    We present a compact, fast, and versatile fiber-optic probe system for real-time determination of tissue optical properties from spatially resolved continuous-wave diffuse reflectance measurements. The system collects one set of reflectance data from six source-detector distances at four arbitrary wavelengths with a maximum overall sampling rate of 100 Hz. Multivariate calibration techniques based on two-dimensional polynomial fitting are employed to extract and display the absorption and reduced scattering coefficients in real-time mode. The four wavelengths of the current configuration are 660, 785, 805, and 974 nm, respectively. Cross-validation tests on a 6 x 7 calibration matrix of Intralipid-dye phantoms showed that the mean prediction error at, e.g., 785 nm was 2.8% for the absorption coefficient and 1.3% for the reduced scattering coefficient. The errors are relative to the range of the optical properties of the phantoms at 785 nm, which were 0-0.3/cm for the absorption coefficient and 6-16/cm for the reduced scattering coefficient. Finally, we also present and discuss results from preliminary skin tissue measurements.

  18. Sensitive on-chip quantitative real-time PCR performed on an adaptable and robust platform.

    PubMed

    Lund-Olesen, Torsten; Dufva, Martin; Dahl, John Arne; Collas, Philippe; Hansen, Mikkel Fougt

    2008-12-01

    A robust, flexible and efficient system for performing high sensitivity quantitative on-chip real-time PCR for research purposes is presented. The chips used consist of microchannels etched in silicon. The surface in the channels is a thermally grown silicon dioxide and the channel is sealed by a glass lid. The chips contain four PCR chambers but this number can be increased for further multiplexing. Contrary to PCR chips with oil covered open chambers, these channel-like chambers are easily integrated in lab-on-a-chip devices. The temperature is controlled by a Peltier element and the fluorochrome detector system is a commercially available fluorescence stereo microscope equipped with a CCD camera. The setup shows an excellent signal-to-noise ratio of about 400 compared to that of about 150 obtained in a commercial real time PCR machine. A detection limit of a few copies of target molecules is found, which is 100 to 100,000-fold better than other on-chip real-time PCR systems presented in the literature. This demonstrates that the PCR system can be used for critical applications. We also demonstrate that high quality melting curves can be obtained. Such curves are important in lab-on-a-chip systems for identification of amplified product. The usability of the system is validated by performing quantitative on-chip measurements of the amount of specific gene sequences co-immunoprecipitated with various posttranslationally modified histone proteins. Similar results are obtained from on-chip experiments and experiments carried out in a commercial system on larger sample volumes.

  19. Legionellosis and Lung Abscesses: Contribution of Legionella Quantitative Real-Time PCR to an Adapted Followup

    PubMed Central

    Descours, G.; Tellini, C.; Flamens, C.; Philit, F.; Celard, M.; Etienne, J.; Lina, G.; Jarraud, S.

    2013-01-01

    We report a case of severe Legionnaires' disease (LD) complicated by a lung abscess in an immunocompetent patient who required ECMO therapy and thoracic surgery. The results of repeated Legionella quantitative real-time PCR performed on both sera and respiratory samples correlated with the LD severity and the poor clinical outcome. Moreover, the PCR allowed for the detection of Legionella DNA in the lung abscess specimen, which was negative when cultured for Legionella. This case report provides a logical basis for further investigations to examine whether the Legionella quantitative PCR could improve the assessment of LD severity and constitute a prognostic marker. PMID:23862082

  20. Adaptive Interface Approach Using a Real Time Biocybernetic System: Control of Hazardous Awareness

    NASA Technical Reports Server (NTRS)

    Ray, William J.

    2002-01-01

    The focus of this current grant was to continue our work which focused on the manner in which psychophysiological markers can be used to index hazardous states of awareness and to explore the feasibility of developing on-line systems that utilize real time feedback to modify on-going behavioral processes. In this work we have incorporated a multifaceted approach which includes psychophysiological, subjective, and performance based measures. We have considered this from both an internal and external perspective as reflected in work from a variety of labs.

  1. An SDR-Based Real-Time Testbed for GNSS Adaptive Array Anti-Jamming Algorithms Accelerated by GPU.

    PubMed

    Xu, Hailong; Cui, Xiaowei; Lu, Mingquan

    2016-03-11

    Nowadays, software-defined radio (SDR) has become a common approach to evaluate new algorithms. However, in the field of Global Navigation Satellite System (GNSS) adaptive array anti-jamming, previous work has been limited due to the high computational power demanded by adaptive algorithms, and often lack flexibility and configurability. In this paper, the design and implementation of an SDR-based real-time testbed for GNSS adaptive array anti-jamming accelerated by a Graphics Processing Unit (GPU) are documented. This testbed highlights itself as a feature-rich and extendible platform with great flexibility and configurability, as well as high computational performance. Both Space-Time Adaptive Processing (STAP) and Space-Frequency Adaptive Processing (SFAP) are implemented with a wide range of parameters. Raw data from as many as eight antenna elements can be processed in real-time in either an adaptive nulling or beamforming mode. To fully take advantage of the parallelism resource provided by the GPU, a batched method in programming is proposed. Tests and experiments are conducted to evaluate both the computational and anti-jamming performance. This platform can be used for research and prototyping, as well as a real product in certain applications.

  2. An SDR-Based Real-Time Testbed for GNSS Adaptive Array Anti-Jamming Algorithms Accelerated by GPU

    PubMed Central

    Xu, Hailong; Cui, Xiaowei; Lu, Mingquan

    2016-01-01

    Nowadays, software-defined radio (SDR) has become a common approach to evaluate new algorithms. However, in the field of Global Navigation Satellite System (GNSS) adaptive array anti-jamming, previous work has been limited due to the high computational power demanded by adaptive algorithms, and often lack flexibility and configurability. In this paper, the design and implementation of an SDR-based real-time testbed for GNSS adaptive array anti-jamming accelerated by a Graphics Processing Unit (GPU) are documented. This testbed highlights itself as a feature-rich and extendible platform with great flexibility and configurability, as well as high computational performance. Both Space-Time Adaptive Processing (STAP) and Space-Frequency Adaptive Processing (SFAP) are implemented with a wide range of parameters. Raw data from as many as eight antenna elements can be processed in real-time in either an adaptive nulling or beamforming mode. To fully take advantage of the parallelism resource provided by the GPU, a batched method in programming is proposed. Tests and experiments are conducted to evaluate both the computational and anti-jamming performance. This platform can be used for research and prototyping, as well as a real product in certain applications. PMID:26978363

  3. Adapting CALIPSO Climate Measurements for Near Real Time Analyses and Forecasting

    NASA Technical Reports Server (NTRS)

    Vaughan, Mark A.; Trepte, Charles R.; Winker, David M.; Avery, Melody A.; Campbell, James; Hoff, Ray; Young, Stuart; Getzewich, Brian J.; Tackett, Jason L.; Kar, Jayanta

    2011-01-01

    The Cloud-Aerosol Lidar and Infrared Pathfinder satellite Observations (CALIPSO) mission was originally conceived and designed as a climate measurements mission, with considerable latency between data acquisition and the release of the level 1 and level 2 data products. However, the unique nature of the CALIPSO lidar backscatter profiles quickly led to the qualitative use of CALIPSO?s near real time (i.e., ? expedited?) lidar data imagery in several different forecasting applications. To enable quantitative use of their near real time analyses, the CALIPSO project recently expanded their expedited data catalog to include all of the standard level 1 and level 2 lidar data products. Also included is a new cloud cleared level 1.5 profile product developed for use by operational forecast centers for verification of aerosol predictions. This paper describes the architecture and content of the CALIPSO expedited data products. The fidelity and accuracy of the expedited products are assessed via comparisons to the standard CALIPSO data products.

  4. An Adaptive Non-Local-Means Filter for Real-Time MR-Thermometry.

    PubMed

    Zachiu, Cornel; Ries, Mario; Moonen, Chrit; de Senneville, Baudouin Denis

    2017-04-01

    Proton resonance frequency shift-based magnetic resonance thermometry is a currently used technique for monitoring temperature during targeted thermal therapies. However, in order to provide temperature updates with very short latency times, fast MR acquisition schemes are usually employed, which in turn might lead to noisy temperature measurements. This will, in general, have a direct impact on therapy control and endpoint detection. In this paper, we address this problem through an improved non-local filtering technique applied on the temperature images. Compared with previous non-local filtering methods, the proposed approach considers not only spatial information but also exploits temporal redundancies. The method is fully automatic and designed to improve the precision of the temperature measurements while at the same time maintaining output accuracy. In addition, the implementation was optimized in order to ensure real-time availability of the temperature measurements while having a minimal impact on latency. The method was validated in three complementary experiments: a simulation, an ex-vivo and an in-vivo study. Compared to the original non-local means filter and two other previously employed temperature filtering methods, the proposed approach shows considerable improvement in both accuracy and precision of the filtered data. Together with the low computational demands of the numerical scheme, the proposed filtering technique shows great potential for improving temperature measurements during real-time MR thermometry dedicated to targeted thermal therapies.

  5. Real-time in vivo diagnosis of laryngeal carcinoma with rapid fiber-optic Raman spectroscopy.

    PubMed

    Lin, Kan; Zheng, Wei; Lim, Chwee Ming; Huang, Zhiwei

    2016-09-01

    We assess the clinical utility of a unique simultaneous fingerprint (FP) (i.e., 800-1800 cm(-1)) and high-wavenumber (HW) (i.e., 2800-3600 cm(-1)) fiber-optic Raman spectroscopy for in vivo diagnosis of laryngeal cancer at endoscopy. A total of 2124 high-quality in vivo FP/HW Raman spectra (normal = 1321; cancer = 581) were acquired from 101 tissue sites (normal = 71; cancer = 30) of 60 patients (normal = 44; cancer = 16) undergoing routine endoscopic examination. FP/HW Raman spectra differ significantly between normal and cancerous laryngeal tissue that could be attributed to changes of proteins, lipids, nucleic acids, and the bound water content in the larynx. Partial least squares-discriminant analysis and leave-one tissue site-out, cross-validation were employed on the in vivo FP/HW tissue Raman spectra acquired, yielding a diagnostic accuracy of 91.1% (sensitivity: 93.3% (28/30); specificity: 90.1% (64/71)) for laryngeal cancer identification, which is superior to using either FP (accuracy: 86.1%; sensitivity: 86.7% (26/30); specificity: 85.9% (61/71)) or HW (accuracy: 84.2%; sensitivity: 76.7% (23/30); specificity: 87.3% (62/71)) Raman technique alone. Further receiver operating characteristic analysis reconfirms the best performance of the simultaneous FP/HW Raman technique for laryngeal cancer diagnosis. We demonstrate for the first time that the simultaneous FP/HW Raman spectroscopy technique can be used for improving real-time in vivo diagnosis of laryngeal carcinoma during endoscopic examination.

  6. Clinical development of BLZ-100 for real-time optical imaging of tumors during resection

    NASA Astrophysics Data System (ADS)

    Franklin, Heather L.; Miller, Dennis M.; Hedges, Teresa; Perry, Jeff; Parrish-Novak, Julia

    2016-03-01

    Complete initial resection can give cancer patients the best opportunity for long-term survival. There is unmet need in surgical oncology for optical imaging that enables simple and precise visualization of tumors and consistent contrast with surrounding normal tissues. Near-infrared (NIR) contrast agents and camera systems that can detect them represent an area of active research and development. The investigational Tumor Paint agent BLZ-100 is a conjugate of a chlorotoxin peptide and the NIR dye indocyanine green (ICG) that has been shown to specifically bind to a broad range of solid tumors. Clinical efficacy studies with BLZ-100 are in progress, a necessary step in bringing the product into clinical practice. To ensure a product that will be useful for and accepted by surgeons, the early clinical development of BLZ- 100 incorporates multiple tumor types and imaging devices so that surgeon feedback covers the range of anticipated clinical uses. Key contrast agent characteristics include safety, specificity, flexibility in timing between dose and surgery, and breadth of tumor types recognized. Imaging devices should use wavelengths that are optimal for the contrast agent, be sensitive enough that contrast agent dosing can be adjusted for optimal contrast, include real-time video display of fluorescence and white light image, and be simple for surgeons to use with minimal disruption of surgical flow. Rapid entry into clinical studies provides the best opportunity for early surgeon feedback, enabling development of agents and devices that will gain broad acceptance and provide information that helps surgeons achieve more complete and precise resections.

  7. Real-Time Automatic Segmentation of Optical Coherence Tomography Volume Data of the Macular Region

    PubMed Central

    Tian, Jing; Varga, Boglárka; Somfai, Gábor Márk; Lee, Wen-Hsiang; Smiddy, William E.; Cabrera DeBuc, Delia

    2015-01-01

    Optical coherence tomography (OCT) is a high speed, high resolution and non-invasive imaging modality that enables the capturing of the 3D structure of the retina. The fast and automatic analysis of 3D volume OCT data is crucial taking into account the increased amount of patient-specific 3D imaging data. In this work, we have developed an automatic algorithm, OCTRIMA 3D (OCT Retinal IMage Analysis 3D), that could segment OCT volume data in the macular region fast and accurately. The proposed method is implemented using the shortest-path based graph search, which detects the retinal boundaries by searching the shortest-path between two end nodes using Dijkstra’s algorithm. Additional techniques, such as inter-frame flattening, inter-frame search region refinement, masking and biasing were introduced to exploit the spatial dependency between adjacent frames for the reduction of the processing time. Our segmentation algorithm was evaluated by comparing with the manual labelings and three state of the art graph-based segmentation methods. The processing time for the whole OCT volume of 496×644×51 voxels (captured by Spectralis SD-OCT) was 26.15 seconds which is at least a 2-8-fold increase in speed compared to other, similar reference algorithms used in the comparisons. The average unsigned error was about 1 pixel (∼ 4 microns), which was also lower compared to the reference algorithms. We believe that OCTRIMA 3D is a leap forward towards achieving reliable, real-time analysis of 3D OCT retinal data. PMID:26258430

  8. Super continuum generation for real time ultrahigh resolution optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Nishizawa, Norihiko; Aguirre, Aaron D.; Fujimoto, James G.

    2006-02-01

    Optical coherence tomography (OCT) is an emerging technology for micrometer-scale, cross-sectional imaging of biological tissue and materials. One of the key limitations to achieving ultrahigh-resolution OCT imaging outside the laboratory setting has been the lack of compact, high-performance broadband light sources with sufficient power and stability to allow practical real-time imaging. The broad-bandwidth supercontinuum (SC) sources were recently demonstrated with femtosecond lasers in combination with nonlinear fibers. Using SC, we can demonstrate ultrahigh resolution OCT. However, wideband SC generally has large excess noise and significant fine structure. Low noise and smooth spectral shape are desired in the ideal supercontinnum source. In this paper, we describe recent studies on practical SC generation for ultrahigh-resolution OCT. SC generation is first analyzed both numerically and experimentally in terms of OCT imaging requirements and optimized conditions for generation are discussed. Supercontinua generated by use of highly nonlinear fiber which have a zero-dispersion wavelength near the pump wavelength, generally result in severe spectral modulation and fluctuating fine structure in the spectra. This spectral modulation produces sidelobes and reduced contrast in the interferometric point-spread function. In contrast, normally dispersive, highly nonlinear fibers (ND-HNFs) can generate smooth and Gaussian shaped supercontinua by the combination of self-phase modulation and normal dispersion. Low noise and wideband SC generation is demonstrated using ND-HNFs. Two colored SC generation is also demonstrated using a photonic crystal fiber which has two close zero dispersion wavelengths. The numerical results are almost in agreement with the experimental ones. Finally, low noise SC generation is demonstrated in an all fiber system based on an ultrashort pulse fiber laser. Wideband, low noise, near Gaussian shaped, high power SC is generated in the 1.55

  9. Real-time 3D Fourier-domain optical coherence tomography guided microvascular anastomosis

    NASA Astrophysics Data System (ADS)

    Huang, Yong; Ibrahim, Zuhaib; Lee, W. P. A.; Brandacher, Gerald; Kang, Jin U.

    2013-03-01

    Vascular and microvascular anastomosis is considered to be the foundation of plastic and reconstructive surgery, hand surgery, transplant surgery, vascular surgery and cardiac surgery. In the last two decades innovative techniques, such as vascular coupling devices, thermo-reversible poloxamers and suture-less cuff have been introduced. Intra-operative surgical guidance using a surgical imaging modality that provides in-depth view and 3D imaging can improve outcome following both conventional and innovative anastomosis techniques. Optical coherence tomography (OCT) is a noninvasive high-resolution (micron level), high-speed, 3D imaging modality that has been adopted widely in biomedical and clinical applications. In this work we performed a proof-of-concept evaluation study of OCT as an assisted intraoperative and post-operative imaging modality for microvascular anastomosis of rodent femoral vessels. The OCT imaging modality provided lateral resolution of 12 μm and 3.0 μm axial resolution in air and 0.27 volume/s imaging speed, which could provide the surgeon with clearly visualized vessel lumen wall and suture needle position relative to the vessel during intraoperative imaging. Graphics processing unit (GPU) accelerated phase-resolved Doppler OCT (PRDOCT) imaging of the surgical site was performed as a post-operative evaluation of the anastomosed vessels and to visualize the blood flow and thrombus formation. This information could help surgeons improve surgical precision in this highly challenging anastomosis of rodent vessels with diameter less than 0.5 mm. Our imaging modality could not only detect accidental suture through the back wall of lumen but also promptly diagnose and predict thrombosis immediately after reperfusion. Hence, real-time OCT can assist in decision-making process intra-operatively and avoid post-operative complications.

  10. Real-Time Optical Monitoring of Flow Kinetics and Gas Phase Reactions Under High-Pressure OMCVD Conditions

    NASA Technical Reports Server (NTRS)

    Dietz, N.; McCall, S.; Bachmann, K. J.

    2001-01-01

    This contribution addresses the real-time optical characterization of gas flow and gas phase reactions as they play a crucial role for chemical vapor phase depositions utilizing elevated and high pressure chemical vapor deposition (HPCVD) conditions. The objectives of these experiments are to validate on the basis of results on real-time optical diagnostics process models simulation codes, and provide input parameter sets needed for analysis and control of chemical vapor deposition at elevated pressures. Access to microgravity is required to retain high pressure conditions of laminar flow, which is essential for successful acquisition and interpretation of the optical data. In this contribution, we describe the design and construction of the HPCVD system, which include access ports for various optical methods of real-time process monitoring and to analyze the initial stages of heteroepitaxy and steady-state growth in the different pressure ranges. To analyze the onset of turbulence, provisions are made for implementation of experimental methods for in-situ characterization of the nature of flow. This knowledge will be the basis for the design definition of experiments under microgravity, where gas flow conditions, gas phase and surface chemistry, might be analyzed by remote controlled real-time diagnostics tools, developed in this research project.

  11. Adaptive time-domain filtering for real-time spectral discrimination in a Michelson interferometer.

    PubMed

    Bhalotra, Sameer R; Kung, Helen L; Jiao, Yang; Miller, David A B

    2002-07-01

    We present a method of spectral discrimination that employs time-domain processing instead of the typical frequency-domain analysis and implement the method in a Michelson interferometer with a nonlinear mirror scan. The technique yields one analog output value per scan instead of a complete interferogram by directly filtering a measured scan with a reference function in the time domain. Such a procedure drastically reduces data-processing requirements downstream. Additionally, using prerecorded interferograms as references eliminates the need to compensate for scan nonlinearities, which broadens the field of usable components for implementation in miniaturized sensing systems. With our efficient use of known spectral signatures, we demonstrate real-time discrimination of 633- and 663-nm laser sources with a mirror scan length of 1 microm , compared with the Rayleigh criterion of 7 microm.

  12. Characterization, adaptive traffic shaping, and multiplexing of real-time MPEG II video

    NASA Astrophysics Data System (ADS)

    Agrawal, Sanjay; Barry, Charles F.; Binnai, Vinay; Kazovsky, Leonid G.

    1997-01-01

    We obtain network traffic model for real-time MPEG-II encoded digital video by analyzing video stream samples from real-time encoders from NUKO Information Systems. MPEG-II sample streams include a resolution intensive movie, City of Joy, an action intensive movie, Aliens, a luminance intensive (black and white) movie, Road To Utopia, and a chrominance intensive (color) movie, Dick Tracy. From our analysis we obtain a heuristic model for the encoded video traffic which uses a 15-stage Markov process to model the I,B,P frame sequences within a group of pictures (GOP). A jointly-correlated Gaussian process is used to model the individual frame sizes. Scene change arrivals are modeled according to a gamma process. Simulations show that our MPEG-II traffic model generates, I,B,P frame sequences and frame sizes that closely match the sample MPEG-II stream traffic characteristics as they relate to latency and buffer occupancy in network queues. To achieve high multiplexing efficiency we propose a traffic shaping scheme which sets preferred 1-frame generation times among a group of encoders so as to minimize the overall variation in total offered traffic while still allowing the individual encoders to react to scene changes. Simulations show that our scheme results in multiplexing gains of up to 10% enabling us to multiplex twenty 6 Mbps MPEG-II video streams instead of 18 streams over an ATM/SONET OC3 link without latency or cell loss penalty. This scheme is due for a patent.

  13. Flexible adaptive paradigms for fMRI using a novel software package 'Brain Analysis in Real-Time' (BART).

    PubMed

    Hellrung, Lydia; Hollmann, Maurice; Zscheyge, Oliver; Schlumm, Torsten; Kalberlah, Christian; Roggenhofer, Elisabeth; Okon-Singer, Hadas; Villringer, Arno; Horstmann, Annette

    2015-01-01

    In this work we present a new open source software package offering a unified framework for the real-time adaptation of fMRI stimulation procedures. The software provides a straightforward setup and highly flexible approach to adapt fMRI paradigms while the experiment is running. The general framework comprises the inclusion of parameters from subject's compliance, such as directing gaze to visually presented stimuli and physiological fluctuations, like blood pressure or pulse. Additionally, this approach yields possibilities to investigate complex scientific questions, for example the influence of EEG rhythms or fMRI signals results themselves. To prove the concept of this approach, we used our software in a usability example for an fMRI experiment where the presentation of emotional pictures was dependent on the subject's gaze position. This can have a significant impact on the results. So far, if this is taken into account during fMRI data analysis, it is commonly done by the post-hoc removal of erroneous trials. Here, we propose an a priori adaptation of the paradigm during the experiment's runtime. Our fMRI findings clearly show the benefits of an adapted paradigm in terms of statistical power and higher effect sizes in emotion-related brain regions. This can be of special interest for all experiments with low statistical power due to a limited number of subjects, a limited amount of time, costs or available data to analyze, as is the case with real-time fMRI.

  14. Adaptive Liquid Crystal TV Based Joint Transform Correlator as Applied to Real-Time Pattern Recognition

    DTIC Science & Technology

    1991-05-23

    F.T.S. Yu and T. Nagata, "Binary Phase only Joint Transform Correlator," Microwave and Optical Technology Letters, Vol. 2, pp. 15-19, January 1989. F...LCTVs,* Microwave and Optical Technology Letters, pp. 309--1-313, I T. Lu, S. WU. X. Xii and F.T.S. Nru, "Optical Implementation 01 Programmable...smeared image is published in Microwave and Optical Technology Letters [14]. 3 9.11 Reconfigurable Interconnections (APPENDIX 10.11) High speed

  15. Real time optical Biopsy: Time-resolved Fluorescence Spectroscopy instrumentation and validation

    PubMed Central

    Kittle, David S.; Vasefi, Fartash; Patil, Chirag G.; Mamelak, Adam; Black, Keith L.; Butte, Pramod V.

    2016-01-01

    The Time-resolved fluorescence spectroscopy (TR-FS) has the potential to differentiate tumor and normal tissue in real time during surgical excision. In this manuscript, we describe the design of a novel TR-FS device, along with preliminary data on detection accuracy for fluorophores in a mixture. The instrument is capable of near real-time fluorescence lifetime acquisition in multiple spectral bands and analysis. It is also able to recover fluorescence lifetime with sub-20ps accuracy as validated with individual organic fluorescence dyes and dye mixtures yielding lifetime values for standard fluorescence dyes that closely match with published data. We also show that TR-FS is able to quantify the relative concentration of fluorescence dyes in a mixture by the unmixing of lifetime decays. We show that the TR-FS prototype is able to identify in near-real time the concentrations of dyes in a complex mixture based on previously trained data. As a result, we demonstrate that in complex mixtures of fluorophores, the relative concentration information is encoded in the fluorescence lifetime across multiple spectral bands. We show for the first time the temporal and spectral measurements of a mixture of fluorochromes and the ability to differentiate relative concentrations of each fluorochrome mixture in real time. PMID:27929039

  16. Real time optical Biopsy: Time-resolved Fluorescence Spectroscopy instrumentation and validation.

    PubMed

    Kittle, David S; Vasefi, Fartash; Patil, Chirag G; Mamelak, Adam; Black, Keith L; Butte, Pramod V

    2016-12-08

    The Time-resolved fluorescence spectroscopy (TR-FS) has the potential to differentiate tumor and normal tissue in real time during surgical excision. In this manuscript, we describe the design of a novel TR-FS device, along with preliminary data on detection accuracy for fluorophores in a mixture. The instrument is capable of near real-time fluorescence lifetime acquisition in multiple spectral bands and analysis. It is also able to recover fluorescence lifetime with sub-20ps accuracy as validated with individual organic fluorescence dyes and dye mixtures yielding lifetime values for standard fluorescence dyes that closely match with published data. We also show that TR-FS is able to quantify the relative concentration of fluorescence dyes in a mixture by the unmixing of lifetime decays. We show that the TR-FS prototype is able to identify in near-real time the concentrations of dyes in a complex mixture based on previously trained data. As a result, we demonstrate that in complex mixtures of fluorophores, the relative concentration information is encoded in the fluorescence lifetime across multiple spectral bands. We show for the first time the temporal and spectral measurements of a mixture of fluorochromes and the ability to differentiate relative concentrations of each fluorochrome mixture in real time.

  17. Real time optical Biopsy: Time-resolved Fluorescence Spectroscopy instrumentation and validation

    NASA Astrophysics Data System (ADS)

    Kittle, David S.; Vasefi, Fartash; Patil, Chirag G.; Mamelak, Adam; Black, Keith L.; Butte, Pramod V.

    2016-12-01

    The Time-resolved fluorescence spectroscopy (TR-FS) has the potential to differentiate tumor and normal tissue in real time during surgical excision. In this manuscript, we describe the design of a novel TR-FS device, along with preliminary data on detection accuracy for fluorophores in a mixture. The instrument is capable of near real-time fluorescence lifetime acquisition in multiple spectral bands and analysis. It is also able to recover fluorescence lifetime with sub-20ps accuracy as validated with individual organic fluorescence dyes and dye mixtures yielding lifetime values for standard fluorescence dyes that closely match with published data. We also show that TR-FS is able to quantify the relative concentration of fluorescence dyes in a mixture by the unmixing of lifetime decays. We show that the TR-FS prototype is able to identify in near-real time the concentrations of dyes in a complex mixture based on previously trained data. As a result, we demonstrate that in complex mixtures of fluorophores, the relative concentration information is encoded in the fluorescence lifetime across multiple spectral bands. We show for the first time the temporal and spectral measurements of a mixture of fluorochromes and the ability to differentiate relative concentrations of each fluorochrome mixture in real time.

  18. [Adaptation of a sensitive DNA extraction method for detection of Entamoeba histolytica by real-time polymerase chain reaction].

    PubMed

    Pınar, Ahmet; Akyön, Yakut; Alp, Alpaslan; Ergüven, Sibel

    2010-07-01

    This study was aimed to adapt a sensitive DNA extraction protocol in stool samples for real-time polymerase chain reaction (PCR) detection of Entamoeba histolytica which causes important morbidity and mortality worldwide. Stool extraction is a problematic step and has direct effects on PCR sensitivity. In order to improve the sensitivity of E.histolytica detection by real-time PCR, "QIAamp DNA stool minikit (Qiagen, Germany)" was modified by adding an overnight incubation step with proteinase K and sodium dodecyl sulfate (SDS) in this study. Three different extraction methods [(1) original method, (2) cetyltrimethyl-ammonium bromide (CTAB) method, (3) modified method] were evaluated for effects on sensitivity in real-time quantitative PCR (Artus RealArt TM E.histolytica RG PCR Kit, Qiagen Diagnostics, Germany). For this purpose, several concentrations of standard E.histolytica DNA were spiked in parasite-free stool samples and three different extraction protocols were performed. Detection sensitivities of "QIAamp DNA stool minikit" was found 5000 copies/ml and of CTAB method was found 500 copies/ml. Detection sensitivity of the extraction was improved to 5 copies/mL by modified "QIAamp DNA stool minikit" protocol. Since detection sensitivities of nucleic acid extraction protocols from stool samples directly affect the sensitivity of PCR amplification, different extraction protocols for different microorganisms should be evaluated.

  19. Neuromorphic learning of continuous-valued mappings in the presence of noise: Application to real-time adaptive control

    NASA Technical Reports Server (NTRS)

    Troudet, Terry; Merrill, Walter C.

    1989-01-01

    The ability of feed-forward neural net architectures to learn continuous-valued mappings in the presence of noise is demonstrated in relation to parameter identification and real-time adaptive control applications. Factors and parameters influencing the learning performance of such nets in the presence of noise are identified. Their effects are discussed through a computer simulation of the Back-Error-Propagation algorithm by taking the example of the cart-pole system controlled by a nonlinear control law. Adequate sampling of the state space is found to be essential for canceling the effect of the statistical fluctuations and allowing learning to take place.

  20. Real-Time Molecular Monitoring of Chemical Environment in ObligateAnaerobes during Oxygen Adaptive Response

    SciTech Connect

    Holman, Hoi-Ying N.; Wozei, Eleanor; Lin, Zhang; Comolli, Luis R.; Ball, David. A.; Borglin, Sharon; Fields, Matthew W.; Hazen, Terry C.; Downing, Kenneth H.

    2009-02-25

    Determining the transient chemical properties of the intracellular environment canelucidate the paths through which a biological system adapts to changes in its environment, for example, the mechanisms which enable some obligate anaerobic bacteria to survive a sudden exposure to oxygen. Here we used high-resolution Fourier Transform Infrared (FTIR) spectromicroscopy to continuously follow cellular chemistry within living obligate anaerobes by monitoring hydrogen bonding in their cellular water. We observed a sequence of wellorchestrated molecular events that correspond to changes in cellular processes in those cells that survive, but only accumulation of radicals in those that do not. We thereby can interpret the adaptive response in terms of transient intracellular chemistry and link it to oxygen stress and survival. This ability to monitor chemical changes at the molecular level can yield important insights into a wide range of adaptive responses.

  1. Real-time 3D adaptive filtering for portable imaging systems

    NASA Astrophysics Data System (ADS)

    Bockenbach, Olivier; Ali, Murtaza; Wainwright, Ian; Nadeski, Mark

    2015-03-01

    Portable imaging devices have proven valuable for emergency medical services both in the field and hospital environments and are becoming more prevalent in clinical settings where the use of larger imaging machines is impractical. 3D adaptive filtering is one of the most advanced techniques aimed at noise reduction and feature enhancement, but is computationally very demanding and hence often not able to run with sufficient performance on a portable platform. In recent years, advanced multicore DSPs have been introduced that attain high processing performance while maintaining low levels of power dissipation. These processors enable the implementation of complex algorithms like 3D adaptive filtering, improving the image quality of portable medical imaging devices. In this study, the performance of a 3D adaptive filtering algorithm on a digital signal processor (DSP) is investigated. The performance is assessed by filtering a volume of size 512x256x128 voxels sampled at a pace of 10 MVoxels/sec.

  2. Closed-loop optical stimulation and recording system with GPU-based real-time spike sorting

    NASA Astrophysics Data System (ADS)

    Wang, Ling; Nguyen, Thoa; Cabral, Henrique; Gysbrechts, Barbara; Battaglia, Francesco; Bartic, Carmen

    2014-05-01

    Closed-loop brain computer interfaces are rapidly progressing due to their applications in fundamental neuroscience and prosthetics. For optogenetic experiments, the integration of optical stimulation and electrophysiological recordings is emerging as an imperative engineering research topic. Optical stimulation does not only bring the advantage of cell-type selectivity, but also provides an alternative solution to the electrical stimulation-induced artifacts, a challenge in closedloop architectures. A closed-loop system must identify the neuronal signals in real-time such that a strategy is selected immediately (within a few milliseconds) for delivering stimulation patterns. Real-time spike sorting poses important challenges especially when a large number of recording channels are involved. Here we present a prototype allowing simultaneous optical stimulation and electro-physiological recordings in a closed-loop manner. The prototype was implemented with online spike detection and classification capabilities for selective cell stimulation. Real-time spike sorting was achieved by computations with a high speed, low cost graphic processing unit (GPU). We have successfully demonstrated the closed-loop operation, i.e. optical stimulation in vivo based on spike detection from 8 tetrodes (32 channels). The performance of GPU computation in spike sorting for different channel numbers and signal lengths was also investigated.

  3. Real-Time Feedback Control of Flow-Induced Cavity Tones. Part 2; Adaptive Control

    NASA Technical Reports Server (NTRS)

    Kegerise, M. A.; Cabell, R. H.; Cattafesta, L. N., III

    2006-01-01

    An adaptive generalized predictive control (GPC) algorithm was formulated and applied to the cavity flow-tone problem. The algorithm employs gradient descent to update the GPC coefficients at each time step. Past input-output data and an estimate of the open-loop pulse response sequence are all that is needed to implement the algorithm for application at fixed Mach numbers. Transient measurements made during controller adaptation revealed that the controller coefficients converged to a steady state in the mean, and this implies that adaptation can be turned off at some point with no degradation in control performance. When converged, the control algorithm demonstrated multiple Rossiter mode suppression at fixed Mach numbers ranging from 0.275 to 0.38. However, as in the case of fixed-gain GPC, the adaptive GPC performance was limited by spillover in sidebands around the suppressed Rossiter modes. The algorithm was also able to maintain suppression of multiple cavity tones as the freestream Mach number was varied over a modest range (0.275 to 0.29). Beyond this range, stable operation of the control algorithm was not possible due to the fixed plant model in the algorithm.

  4. Optical aberration correction by real-time holography in liquid crystals.

    PubMed

    Karaguleff, C; Clark, G L

    1990-07-15

    We present results of experiments that use nematic-phase liquid crystal as a real-time holographic recording medium. Plane-wave gratings were written with as little as 10 mJ/cm(2) of incident write-beam energy from a pulsed Nd:YAG laser, and diffraction efficiencies greater than 25% were measured at 633 nm. Grating decay times were observed to fall within two distinct time regimes: a rapid-decay time of 60 to 100 microsec and a slower-decay time of 6 to 30 msec. The rapid-decay regime was used to demonstrate real-time correction of severely aberrated images by degenerate four-wave mixing.

  5. Graphics processing unit-accelerated real-time compressive sensing spectral domain optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Xu, Daguang; Huang, Yong; Kang, Jin U.

    2015-03-01

    In this paper, we systematically demonstrate two real-time CS SD OCT systems based on a conventional desktop having three GPUs. The first one takes fast Fourier transform (FFT) as the sensing technique and under-sampled linear wavenumber spectral sampling as input data, while the second one uses non-uniform fast Fourier transform (NUFFT) and under-sampled nonlinear wavenumber spectral sampling, respectively. The maximum reconstruction speed of 72k and 33.5k A-line/s were achieved for these two systems, respectively, with A-scan size 2048. It is >100 times faster than the C++ implementation and >400 times faster than the MATLAB implementation. Finally, we present real-time dispersion compensated image reconstruction for both systems.

  6. Real-time optical gating for three-dimensional beating heart imaging

    NASA Astrophysics Data System (ADS)

    Taylor, Jonathan M.; Saunter, Christopher D.; Love, Gordon D.; Girkin, John M.; Henderson, Deborah J.; Chaudhry, Bill

    2011-11-01

    We demonstrate real-time microscope image gating to an arbitrary position in the cycle of the beating heart of a zebrafish embryo. We show how this can be used for high-precision prospective gating of fluorescence image slices of the moving heart. We also present initial results demonstrating the application of this technique to 3-D structural imaging of the beating embryonic heart.

  7. Applying RGB LED in full-field optical coherence tomography for real-time full-color tissue imaging.

    PubMed

    Yang, Bor-Wen; Wang, Yu-Yen; Lin, Yu-Min; Juan, Yu-Shan; Chen, Hung-Te; Ying, Shang-Ping

    2014-08-01

    A conventional handheld skin camera is suitable for 2D inspection of shallow skin. Due to its high resolution and noninvasiveness, optical coherence tomography (OCT) has become a popular medical-imaging technology. Among OCT schemes, full-field optical coherence tomography (FF-OCT) is suitable for rapid en face imaging, as it uses a 2D imaging device for pixel processing of a sample plane. Because of its wide bandwidth and long lifetime, an RGB LED was chosen in an FF-OCT system among three source candidates in this study. A full-color tissue image and real-time video were obtained from the system to demonstrate the potential of the RGB LED FF-OCT system in medical imaging. All devices used here can be integrated by micro-optoelectromechanical technology into a handheld model. Noninvasive, real-time, full-color handheld imaging capability contributes to advance dermatology and cosmetology.

  8. Real-Time Tracking Framework with Adaptive Features and Constrained Labels

    PubMed Central

    Li, Daqun; Xu, Tingfa; Chen, Shuoyang; Zhang, Jizhou; Jiang, Shenwang

    2016-01-01

    This paper proposes a novel tracking framework with adaptive features and constrained labels (AFCL) to handle illumination variation, occlusion and appearance changes caused by the variation of positions. The novel ensemble classifier, including the Forward–Backward error and the location constraint is applied, to get the precise coordinates of the promising bounding boxes. The Forward–Backward error can enhance the adaptation and accuracy of the binary features, whereas the location constraint can overcome the label noise to a certain degree. We use the combiner which can evaluate the online templates and the outputs of the classifier to accommodate the complex situation. Evaluation of the widely used tracking benchmark shows that the proposed framework can significantly improve the tracking accuracy, and thus reduce the processing time. The proposed framework has been tested and implemented on the embedded system using TMS320C6416 and Cyclone Ⅲ kernel processors. The outputs show that achievable and satisfying results can be obtained. PMID:27618052

  9. Real-time Adaptive Kinematic Model Estimation of Concentric Tube Robots

    PubMed Central

    Kim, Chunwoo; Ryu, Seok Chang; Dupont, Pierre E.

    2016-01-01

    Kinematic models of concentric tube robots have matured from considering only tube bending to considering tube twisting as well as external loading. While these models have been demonstrated to approximate actual behavior, modeling error can be significant for medical applications that often call for positioning accuracy of 1–2mm. As an alternative to moving to more complex models, this paper proposes using sensing to adaptively update model parameters during robot operation. Advantages of this method are that the model is constantly tuning itself to provide high accuracy in the region of the workspace where it is currently operating. It also adapts automatically to changes in robot shape and compliance associated with the insertion and removal of tools through its lumen. As an initial exploration of this approach, a recursive on-line estimator is proposed and evaluated experimentally. PMID:27175307

  10. Real-time Adaptive Kinematic Model Estimation of Concentric Tube Robots.

    PubMed

    Kim, Chunwoo; Ryu, Seok Chang; Dupont, Pierre E

    2015-01-01

    Kinematic models of concentric tube robots have matured from considering only tube bending to considering tube twisting as well as external loading. While these models have been demonstrated to approximate actual behavior, modeling error can be significant for medical applications that often call for positioning accuracy of 1-2mm. As an alternative to moving to more complex models, this paper proposes using sensing to adaptively update model parameters during robot operation. Advantages of this method are that the model is constantly tuning itself to provide high accuracy in the region of the workspace where it is currently operating. It also adapts automatically to changes in robot shape and compliance associated with the insertion and removal of tools through its lumen. As an initial exploration of this approach, a recursive on-line estimator is proposed and evaluated experimentally.

  11. Real-time optical multiple-object recognition and tracking demonstration: A friendly challenge to the digital field

    NASA Technical Reports Server (NTRS)

    Chao, Tien-Hsin; Liu, Hua-Kuang

    1980-01-01

    Researchers demonstrated the first optical multiple object tracking system. The system is capable of simultaneous tracking of multiple objects, each with independent movements in real-time, limited only to the TV frame rate (30 msec). In order to perform a similar tracking operation, a large computer system and very complex software would be needed. Although researchers have demonstrated the tracking of only 3 objects, the system capacity can easily be expanded by 2 orders of magnitude.

  12. Real-time flatness inspection of rolled products based on optical laser triangulation and three-dimensional surface reconstruction

    NASA Astrophysics Data System (ADS)

    Molleda, Julio; Usamentiaga, Rubén; García, Daniel F.; Bulnes, Francisco G.

    2010-07-01

    Flatness is a major geometrical feature of rolled products specified by both production and quality needs. Real-time inspection of flatness is the basis of automatic flatness control. Industrial facilities where rolled products are manufactured have adverse environments that affect artificial vision systems. We present a low-cost flatness inspection system based on optical triangulation by means of a laser stripe emitter and a CMOS matrix camera, designed to be part of an online flatness control system. An accurate and robust method to extract a laser stripe in adverse conditions over rough surfaces is proposed and designed to be applied in real time. Laser extraction relies on a local and a global search. The global search is based on an adjustment of curve segments based on a split-and-merge technique. A real-time recording method of the input data of the flatness inspection system is proposed. It stores information about manufacturing conditions for an offline tuning of the laser stripe extraction method using real data. Flatness measurements carried out over steel strips are evaluated quantitatively and qualitatively. Moreover, the real-time performance of the proposed system is analyzed.

  13. A real-time photo-realistic rendering algorithm of ocean color based on bio-optical model

    NASA Astrophysics Data System (ADS)

    Ma, Chunyong; Xu, Shu; Wang, Hongsong; Tian, Fenglin; Chen, Ge

    2016-12-01

    A real-time photo-realistic rendering algorithm of ocean color is introduced in the paper, which considers the impact of ocean bio-optical model. The ocean bio-optical model mainly involves the phytoplankton, colored dissolved organic material (CDOM), inorganic suspended particle, etc., which have different contributions to absorption and scattering of light. We decompose the emergent light of the ocean surface into the reflected light from the sun and the sky, and the subsurface scattering light. We establish an ocean surface transmission model based on ocean bidirectional reflectance distribution function (BRDF) and the Fresnel law, and this model's outputs would be the incident light parameters of subsurface scattering. Using ocean subsurface scattering algorithm combined with bio-optical model, we compute the scattering light emergent radiation in different directions. Then, we blend the reflection of sunlight and sky light to implement the real-time ocean color rendering in graphics processing unit (GPU). Finally, we use two kinds of radiance reflectance calculated by Hydrolight radiative transfer model and our algorithm to validate the physical reality of our method, and the results show that our algorithm can achieve real-time highly realistic ocean color scenes.

  14. Real-time error detection but not error correction drives automatic visuomotor adaptation.

    PubMed

    Hinder, Mark R; Riek, Stephan; Tresilian, James R; de Rugy, Aymar; Carson, Richard G

    2010-03-01

    We investigated the role of visual feedback of task performance in visuomotor adaptation. Participants produced novel two degrees of freedom movements (elbow flexion-extension, forearm pronation-supination) to move a cursor towards visual targets. Following trials with no rotation, participants were exposed to a 60 degrees visuomotor rotation, before returning to the non-rotated condition. A colour cue on each trial permitted identification of the rotated/non-rotated contexts. Participants could not see their arm but received continuous and concurrent visual feedback (CF) of a cursor representing limb position or post-trial visual feedback (PF) representing the movement trajectory. Separate groups of participants who received CF were instructed that online modifications of their movements either were, or were not, permissible as a means of improving performance. Feedforward-mediated performance improvements occurred for both CF and PF groups in the rotated environment. Furthermore, for CF participants this adaptation occurred regardless of whether feedback modifications of motor commands were permissible. Upon re-exposure to the non-rotated environment participants in the CF, but not PF, groups exhibited post-training aftereffects, manifested as greater angular deviations from a straight initial trajectory, with respect to the pre-rotation trials. Accordingly, the nature of the performance improvements that occurred was dependent upon the timing of the visual feedback of task performance. Continuous visual feedback of task performance during task execution appears critical in realising automatic visuomotor adaptation through a recalibration of the visuomotor mapping that transforms visual inputs into appropriate motor commands.

  15. A dosimetric comparison of real-time adaptive and non-adaptive radiotherapy: A multi-institutional study encompassing robotic, gimbaled, multileaf collimator and couch tracking

    PubMed Central

    Colvill, Emma; Booth, Jeremy; Nill, Simeon; Fast, Martin; Bedford, James; Oelfke, Uwe; Nakamura, Mitsuhiro; Poulsen, Per; Worm, Esben; Hansen, Rune; Ravkilde, Thomas; Scherman Rydhög, Jonas; Pommer, Tobias; Munck af Rosenschold, Per; Lang, Stephanie; Guckenberger, Matthias; Groh, Christian; Herrmann, Christian; Verellen, Dirk; Poels, Kenneth; Wang, Lei; Hadsell, Michael; Sothmann, Thilo; Blanck, Oliver; Keall, Paul

    2016-01-01

    Purpose A study of real-time adaptive radiotherapy systems was performed to test the hypothesis that, across delivery systems and institutions, the dosimetric accuracy is improved with adaptive treatments over non-adaptive radiotherapy in the presence of patient-measured tumor motion. Methods and materials Ten institutions with robotic(2), gimbaled(2), MLC(4) or couch tracking(2) used common materials including CT and structure sets, motion traces and planning protocols to create a lung and a prostate plan. For each motion trace, the plan was delivered twice to a moving dosimeter; with and without real-time adaptation. Each measurement was compared to a static measurement and the percentage of failed points for γ-tests recorded. Results For all lung traces all measurement sets show improved dose accuracy with a mean 2%/2 mm γ-fail rate of 1.6% with adaptation and 15.2% without adaptation (p < 0.001). For all prostate the mean 2%/2 mm γ-fail rate was 1.4% with adaptation and 17.3% without adaptation (p < 0.001). The difference between the four systems was small with an average 2%/2 mm γ-fail rate of <3% for all systems with adaptation for lung and prostate. Conclusions The investigated systems all accounted for realistic tumor motion accurately and performed to a similar high standard, with real-time adaptation significantly outperforming non-adaptive delivery methods. PMID:27016171

  16. Real-Time Adaptive Control of Flow-Induced Cavity Tones

    NASA Technical Reports Server (NTRS)

    Kegerise, Michael A.; Cabell, Randolph H.; Cattafesta, Louis N.

    2004-01-01

    An adaptive generalized predictive control (GPC) algorithm was formulated and applied to the cavity flow-tone problem. The algorithm employs gradient descent to update the GPC coefficients at each time step. The adaptive control algorithm demonstrated multiple Rossiter mode suppression at fixed Mach numbers ranging from 0.275 to 0.38. The algorithm was also able t o maintain suppression of multiple cavity tones as the freestream Mach number was varied over a modest range (0.275 to 0.29). Controller performance was evaluated with a measure of output disturbance rejection and an input sensitivity transfer function. The results suggest that disturbances entering the cavity flow are colocated with the control input at the cavity leading edge. In that case, only tonal components of the cavity wall-pressure fluctuations can be suppressed and arbitrary broadband pressure reduction is not possible. In the control-algorithm development, the cavity dynamics are treated as linear and time invariant (LTI) for a fixed Mach number. The experimental results lend support this treatment.

  17. Adaptive Liquid Crystal TV Based Joint Transform Correlator as Applied to Real-Time Pattern Recognition

    DTIC Science & Technology

    1993-01-05

    net, in which we assume that the pixel size is 2 mm, the distance between the [TU] plane and the CCD detector is ap - proximately 300 mm and the focal...training patterns. It is ap - parent that ifra pattern belongs to any of the training sets, only the precise primary color IWM would ef- fectively...Appl. Optics 28 (1989) LCT~, mlticannl ner lnet pertion shuld4908. L~T~, mltihanel eurl nt oeraion shuld 151j F.T.S. Yu. T. Lu. X. Yang and D.A

  18. Diffusion-sensitive optical coherence tomography for real-time monitoring of mucus thinning treatments

    PubMed Central

    Blackmon, Richard L.; Kreda, Silvia M.; Sears, Patrick R.; Ostrowski, Lawrence E.; Hill, David B.; Chapman, Brian S.; Tracy, Joseph B.; Oldenburg, Amy L.

    2016-01-01

    Mucus hydration (wt%) has become an increasingly useful metric in real-time assessment of respiratory health in diseases like cystic fibrosis and COPD, with higher wt% indicative of diseased states. However, available in vivo rheological techniques are lacking. Gold nanorods (GNRs) are attractive biological probes whose diffusion through tissue is sensitive to the correlation length of comprising biopolymers. Through employment of dynamic light scattering theory on OCT signals from GNRs, we find that weakly-constrained GNR diffusion predictably decreases with increasing wt% (more disease-like) mucus. Previously, we determined this method is robust against mucus transport on human bronchial epithelial (hBE) air-liquid interface cultures (R2=0.976). Here we introduce diffusion-sensitive OCT (DS-OCT), where we collect M-mode image ensembles, from which we derive depth- and temporally-resolved GNR diffusion rates. DS-OCT allows for real-time monitoring of changing GNR diffusion as a result of topically applied mucus-thinning agents, enabling monitoring of the dynamics of mucus hydration never before seen. Cultured human airway epithelial cells (Calu-3) with a layer of endogenous mucus were doped with topically deposited GNRs (80×22nm), and subsequently treated with hypertonic saline (HS) or isotonic saline (IS). DS-OCT provided imaging of the mucus thinning response up to a depth of 600μm with 4.65μm resolution, over a total of 8 minutes in increments of ≥3 seconds. For both IS and HS conditions, DS-OCT captured changes in the pattern of mucus hydration over time. DS-OCT opens a new window into understanding mechanisms of mucus thinning during treatment, enabling real-time efficacy feedback needed to optimize and tailor treatments for individual patients. PMID:27746581

  19. Diffusion-sensitive optical coherence tomography for real-time monitoring of mucus thinning treatments

    NASA Astrophysics Data System (ADS)

    Blackmon, Richard L.; Kreda, Silvia M.; Sears, Patrick R.; Ostrowski, Lawrence E.; Hill, David B.; Chapman, Brian S.; Tracy, Joseph B.; Oldenburg, Amy L.

    2016-03-01

    Mucus hydration (wt%) has become an increasingly useful metric in real-time assessment of respiratory health in diseases like cystic fibrosis and COPD, with higher wt% indicative of diseased states. However, available in vivo rheological techniques are lacking. Gold nanorods (GNRs) are attractive biological probes whose diffusion through tissue is sensitive to the correlation length of comprising biopolymers. Through employment of dynamic light scattering theory on OCT signals from GNRs, we find that weakly-constrained GNR diffusion predictably decreases with increasing wt% (more disease-like) mucus. Previously, we determined this method is robust against mucus transport on human bronchial epithelial (hBE) air-liquid interface cultures (R2=0.976). Here we introduce diffusion-sensitive OCT (DS-OCT), where we collect M-mode image ensembles, from which we derive depth- and temporally-resolved GNR diffusion rates. DS-OCT allows for real-time monitoring of changing GNR diffusion as a result of topically applied mucus-thinning agents, enabling monitoring of the dynamics of mucus hydration never before seen. Cultured human airway epithelial cells (Calu-3 cell) with a layer of endogenous mucus were doped with topically deposited GNRs (80x22nm), and subsequently treated with hypertonic saline (HS) or isotonic saline (IS). DS-OCT provided imaging of the mucus thinning response up to a depth of 600μm with 4.65μm resolution, over a total of 8 minutes in increments of >=3 seconds. For both IS and HS conditions, DS-OCT captured changes in the pattern of mucus hydration over time. DS-OCT opens a new window into understanding mechanisms of mucus thinning during treatment, enabling real-time efficacy feedback needed to optimize and tailor treatments for individual patients.

  20. Real-time three-dimensional optical coherence tomography image-guided core-needle biopsy system

    PubMed Central

    Kuo, Wei-Cheng; Kim, Jongsik; Shemonski, Nathan D.; Chaney, Eric J.; Spillman, Darold R.; Boppart, Stephen A.

    2012-01-01

    Advances in optical imaging modalities, such as optical coherence tomography (OCT), enable us to observe tissue microstructure at high resolution and in real time. Currently, core-needle biopsies are guided by external imaging modalities such as ultrasound imaging and x-ray computed tomography (CT) for breast and lung masses, respectively. These image-guided procedures are frequently limited by spatial resolution when using ultrasound imaging, or by temporal resolution (rapid real-time feedback capabilities) when using x-ray CT. One feasible approach is to perform OCT within small gauge needles to optically image tissue microstructure. However, to date, no system or core-needle device has been developed that incorporates both three-dimensional OCT imaging and tissue biopsy within the same needle for true OCT-guided core-needle biopsy. We have developed and demonstrate an integrated core-needle biopsy system that utilizes catheter-based 3-D OCT for real-time image-guidance for target tissue localization, imaging of tissue immediately prior to physical biopsy, and subsequent OCT imaging of the biopsied specimen for immediate assessment at the point-of-care. OCT images of biopsied ex vivo tumor specimens acquired during core-needle placement are correlated with corresponding histology, and computational visualization of arbitrary planes within the 3-D OCT volumes enables feedback on specimen tissue type and biopsy quality. These results demonstrate the potential for using real-time 3-D OCT for needle biopsy guidance by imaging within the needle and tissue during biopsy procedures. PMID:22741064

  1. High dynamic range adaptive real-time smart camera: an overview of the HDR-ARTiST project

    NASA Astrophysics Data System (ADS)

    Lapray, Pierre-Jean; Heyrman, Barthélémy; Ginhac, Dominique

    2015-04-01

    Standard cameras capture only a fraction of the information that is visible to the human visual system. This is specifically true for natural scenes including areas of low and high illumination due to transitions between sunlit and shaded areas. When capturing such a scene, many cameras are unable to store the full Dynamic Range (DR) resulting in low quality video where details are concealed in shadows or washed out by sunlight. The imaging technique that can overcome this problem is called HDR (High Dynamic Range) imaging. This paper describes a complete smart camera built around a standard off-the-shelf LDR (Low Dynamic Range) sensor and a Virtex-6 FPGA board. This smart camera called HDR-ARtiSt (High Dynamic Range Adaptive Real-time Smart camera) is able to produce a real-time HDR live video color stream by recording and combining multiple acquisitions of the same scene while varying the exposure time. This technique appears as one of the most appropriate and cheapest solution to enhance the dynamic range of real-life environments. HDR-ARtiSt embeds real-time multiple captures, HDR processing, data display and transfer of a HDR color video for a full sensor resolution (1280 1024 pixels) at 60 frames per second. The main contributions of this work are: (1) Multiple Exposure Control (MEC) dedicated to the smart image capture with alternating three exposure times that are dynamically evaluated from frame to frame, (2) Multi-streaming Memory Management Unit (MMMU) dedicated to the memory read/write operations of the three parallel video streams, corresponding to the different exposure times, (3) HRD creating by combining the video streams using a specific hardware version of the Devebecs technique, and (4) Global Tone Mapping (GTM) of the HDR scene for display on a standard LCD monitor.

  2. Fast optical channel recovery in field demonstration of 100-Gbit/s Ethernet over OTN using real-time DSP.

    PubMed

    Yamazaki, Etsushi; Yamanaka, Shogo; Kisaka, Yoshiaki; Nakagawa, Tadao; Murata, Koichi; Yoshida, Eiji; Sakano, Toshikazu; Tomizawa, Masahito; Miyamoto, Yutaka; Matsuoka, Shinji; Matsui, Junichiro; Shibayama, Atsufumi; Abe, Jun-ichi; Nakamura, Yuichi; Noguchi, Hidemi; Fukuchi, Kiyoshi; Onaka, Hiroshi; Fukumitsu, Katsumi; Komaki, Kousuke; Takeuchi, Osamu; Sakamoto, Yuichiro; Nakashima, Hisao; Mizuochi, Takashi; Kubo, Kazuo; Miyata, Yoshikuni; Nishimoto, Hiroshi; Hirano, Susumu; Onohara, Kiyoshi

    2011-07-04

    A field trial of 100-Gbit/s Ethernet over an optical transport network (OTN) is conducted using a real-time digital coherent signal processor. Error free operation with the Q-margin of 3.2 dB is confirmed at a 100 Gbit/s Ethernet analyzer by concatenating a low-density parity-check code with a OTN framer forward error correction, after 80-ch WDM transmission through 6 spans x 70 km of dispersion shifted fiber without inline-dispersion compensation. Also, the recovery time of 12 msec is observed in an optical route switching experiment, which is achieved through fast chromatic dispersion estimation functionality.

  3. Highly Dynamic and Adaptive Traffic Congestion Avoidance in Real-Time Inspired by Honey Bee Behavior

    NASA Astrophysics Data System (ADS)

    Wedde, Horst F.; Lehnhoff, Sebastian; van Bonn, Bernhard; Bay, Z.; Becker, S.; Böttcher, S.; Brunner, C.; Büscher, A.; Fürst, T.; Lazarescu, A. M.; Rotaru, E.; Senge, S.; Steinbach, B.; Yilmaz, F.; Zimmermann, T.

    Traffic congestions have become a major problem in metropolitan areas world-wide, within and between cities, to an extent where they make driving and transportation times largely unpredictable. Due to the highly dynamic character of congestion building and dissolving this phenomenon appears even to resist a formal treatment. Static approaches, and even more their global management, have proven counterproductive in practice. Given the latest progress in VANET technology and the remarkable commercially driven efforts like in the European C2C consortium, or the VSC Project in the US, allow meanwhile to tackle various aspects of traffic regulation through VANET communication. In this paper we introduce a novel, completely decentralized multi-agent routing algorithm (termed BeeJamA) which we have derived from the foraging behavior of honey bees. It is highly dynamic, adaptive, robust, and scalable, and it allows for both avoiding congestions, and minimizing traveling times to individual destinations. Vehicle guidance is provided well ahead of every intersection, depending on the individual speeds. Thus strict deadlines are imposed on, and respected by, the BeeJamA algorithm. We report on extensive simulation experiments which show the superior performance of BeeJamA over conventional approaches.

  4. Real-time scale-adaptive correlation filters tracker with depth information to handle occlusion

    NASA Astrophysics Data System (ADS)

    Pi, Jiatian; Gu, Yuzhang; Hu, Keli; Cheng, Xiaoliu; Zhan, Yunlong; Wang, Yingguan

    2016-07-01

    In visual object tracking, occlusions significantly undermine the performance of tracking algorithms. RGB-D cameras, such as Microsoft Kinect or the related PrimeSense camera, are widely available to consumers. Great attention has been focused on exploiting depth information for object tracking in recent years. We propose an algorithm that improves the existing correlation filter-based tracker for scale-adaptive tracking. Moreover, we utilize depth information provided by the Kinect camera to handle various types of occlusions. First, the optimal location of the target is obtained by the conventional kernelized correlation filter tracker. Then, we make use of the discriminative correlation filter for scale estimation as an independent part. At last, to further improve the tracking performance under occlusions, we present a simple yet effective occlusion handling mechanism to detect occlusion and recovery. In this mechanism, cluster analysis and object segmentation by K-means method have been applied to depth data. Numerous experiments on Princeton RGB-D tracking dataset demonstrate that the proposed algorithm outperforms several state-of-the-art trackers by successfully dealing with occlusions.

  5. Adaptive real-time bioheat transfer models for computer-driven MR-guided laser induced thermal therapy.

    PubMed

    Fuentes, David; Feng, Yusheng; Elliott, Andrew; Shetty, Anil; McNichols, Roger J; Oden, J Tinsley; Stafford, R J

    2010-05-01

    The treatment times of laser induced thermal therapies (LITT) guided by computational prediction are determined by the convergence behavior of partial differential equation (PDE)-constrained optimization problems. In this paper, we investigate the convergence behavior of a bioheat transfer constrained calibration problem to assess the feasibility of applying to real-time patient specific data. The calibration techniques utilize multiplanar thermal images obtained from the nondestructive in vivo heating of canine prostate. The calibration techniques attempt to adaptively recover the biothermal heterogeneities within the tissue on a patient-specific level and results in a formidable PDE constrained optimization problem to be solved in real time. A comprehensive calibration study is performed with both homogeneous and spatially heterogeneous biothermal model parameters with and without constitutive nonlinearities. Initial results presented here indicate that the calibration problems involving the inverse solution of thousands of model parameters can converge to a solution within three minutes and decrease the [see text for symbol](L) (2) (2) ((0, T; L) (2) ((Omega))) norm of the difference between computational prediction and the measured temperature values to a patient-specific regime.

  6. TaqMan real time RT-PCR assays for detecting ferret innate and adaptive immune responses.

    PubMed

    Carolan, Louise A; Butler, Jeff; Rockman, Steve; Guarnaccia, Teagan; Hurt, Aeron C; Reading, Patrick; Kelso, Anne; Barr, Ian; Laurie, Karen L

    2014-09-01

    The ferret is an excellent model for many human infectious diseases including influenza, SARS-CoV, henipavirus and pneumococcal infections. The ferret is also used to study cystic fibrosis and various cancers, as well as reproductive biology and physiology. However, the range of reagents available to measure the ferret immune response is very limited. To address this deficiency, high-throughput real time RT-PCR TaqMan assays were developed to measure the expression of fifteen immune mediators associated with the innate and adaptive immune responses (IFNα, IFNβ, IFNγ, IL1α, IL1β, IL2, IL4, IL6, IL8, IL10, IL12p40, IL17, Granzyme A, MCP1, TNFα), as well as four endogenous housekeeping genes (ATF4, HPRT, GAPDH, L32). These assays have been optimized to maximize reaction efficiency, reduce the amount of sample required (down to 1ng RNA per real time RT-PCR reaction) and to select the most appropriate housekeeping genes. Using these assays, the expression of each of the tested genes could be detected in ferret lymph node cells stimulated with mitogens or infected with influenza virus in vitro. These new tools will allow a more comprehensive analysis of the ferret immune responses following infection or in other disease states.

  7. Using dual-energy x-ray imaging to enhance automated lung tumor tracking during real-time adaptive radiotherapy

    SciTech Connect

    Menten, Martin J. Fast, Martin F.; Nill, Simeon; Oelfke, Uwe

    2015-12-15

    Purpose: Real-time, markerless localization of lung tumors with kV imaging is often inhibited by ribs obscuring the tumor and poor soft-tissue contrast. This study investigates the use of dual-energy imaging, which can generate radiographs with reduced bone visibility, to enhance automated lung tumor tracking for real-time adaptive radiotherapy. Methods: kV images of an anthropomorphic breathing chest phantom were experimentally acquired and radiographs of actual lung cancer patients were Monte-Carlo-simulated at three imaging settings: low-energy (70 kVp, 1.5 mAs), high-energy (140 kVp, 2.5 mAs, 1 mm additional tin filtration), and clinical (120 kVp, 0.25 mAs). Regular dual-energy images were calculated by weighted logarithmic subtraction of high- and low-energy images and filter-free dual-energy images were generated from clinical and low-energy radiographs. The weighting factor to calculate the dual-energy images was determined by means of a novel objective score. The usefulness of dual-energy imaging for real-time tracking with an automated template matching algorithm was investigated. Results: Regular dual-energy imaging was able to increase tracking accuracy in left–right images of the anthropomorphic phantom as well as in 7 out of 24 investigated patient cases. Tracking accuracy remained comparable in three cases and decreased in five cases. Filter-free dual-energy imaging was only able to increase accuracy in 2 out of 24 cases. In four cases no change in accuracy was observed and tracking accuracy worsened in nine cases. In 9 out of 24 cases, it was not possible to define a tracking template due to poor soft-tissue contrast regardless of input images. The mean localization errors using clinical, regular dual-energy, and filter-free dual-energy radiographs were 3.85, 3.32, and 5.24 mm, respectively. Tracking success was dependent on tumor position, tumor size, imaging beam angle, and patient size. Conclusions: This study has highlighted the influence of

  8. Diffraction-limited real-time terahertz imaging by optical frequency up-conversion in a DAST crystal.

    PubMed

    Fan, Shuzhen; Qi, Feng; Notake, Takashi; Nawata, Kouji; Takida, Yuma; Matsukawa, Takeshi; Minamide, Hiroaki

    2015-03-23

    Real-time terahertz (THz) wave imaging has wide applications in areas such as security, industry, biology, medicine, pharmacy, and the arts. This report describes real-time room-temperature THz imaging by nonlinear optical frequency up-conversion in an organic 4-dimethylamino-N'-methyl-4'-stilbazolium tosylate (DAST) crystal, with high resolution reaching the diffraction limit. THz-wave images were converted to the near infrared region and then captured using an InGaAs camera in a tandem imaging system. The resolution of the imaging system was analyzed. Diffraction and interference of THz wave were observed in the experiments. Videos are supplied to show the interference pattern variation that occurs with sample moving and tilting.

  9. Adaptive optics research at Lincoln Laboratory

    NASA Astrophysics Data System (ADS)

    Greenwood, Darryl P.; Primmerman, Charles A.

    A development history is presented for adaptive-optics methods of optical aberration measurement and correction in real time, which are applicable to the thermal blooming of high-energy laser beams, the compensation of a laser beam propagating from ground to space, and compensation by means of a synthetic beacon. Attention is given to schematics of the various adaptive optics system types, which cover the cases of cooperative and uncooperative targets. Representative research projects encompassed by the high-energy propagation range in West Palm Beach are the 'Everlaser' instrumented target vehicle, the OCULAR multidither system installation, and the Atmospheric Compensation Experiment Adaptive Optics System.

  10. Adaptive marker-free registration using a multiple point strategy for real-time and robust endoscope electromagnetic navigation.

    PubMed

    Luo, Xiongbiao; Wan, Ying; He, Xiangjian; Mori, Kensaku

    2015-02-01

    Registration of pre-clinical images to physical space is indispensable for computer-assisted endoscopic interventions in operating rooms. Electromagnetically navigated endoscopic interventions are increasingly performed at current diagnoses and treatments. Such interventions use an electromagnetic tracker with a miniature sensor that is usually attached at an endoscope distal tip to real time track endoscope movements in a pre-clinical image space. Spatial alignment between the electromagnetic tracker (or sensor) and pre-clinical images must be performed to navigate the endoscope to target regions. This paper proposes an adaptive marker-free registration method that uses a multiple point selection strategy. This method seeks to address an assumption that the endoscope is operated along the centerline of an intraluminal organ which is easily violated during interventions. We introduce an adaptive strategy that generates multiple points in terms of sensor measurements and endoscope tip center calibration. From these generated points, we adaptively choose the optimal point, which is the closest to its assigned the centerline of the hollow organ, to perform registration. The experimental results demonstrate that our proposed adaptive strategy significantly reduced the target registration error from 5.32 to 2.59 mm in static phantoms validation, as well as from at least 7.58 mm to 4.71 mm in dynamic phantom validation compared to current available methods.

  11. Real-Time Wastewater System Operational Strategy Adaptation for Rainfall Variability

    NASA Astrophysics Data System (ADS)

    Zimmer, A. L.; Minsker, B. S.; Schmidt, A.; Ostfeld, A.; Treinish, L. A.

    2010-12-01

    Management strategies for many large-scale wastewater systems are based on longstanding rules and may not effectively utilize system capacity for every storm event. Conservative operational rules are established to prevent flow instabilities. However, the rules may be modified to retain more wastewater and reduce overflows while continuing to avoid hydraulic conditions that lead to transients. Possible adaptation of system decision rules can be evaluated through coupling radar rainfall, a hydrologic system model, and an optimization routine. In this study, genetic algorithm optimization is evaluated for a hydrologic test case modeled after the Chicago Tunnel and Reservoir Plan (TARP) that minimizes combined sewer overflow (CSO) discharge to nearby waterways. This approach identifies potential new operational rules that reliably utilize deep tunnel storage and reduce overflows for a variety of historic storm events. The study utilizes a model based on the portion of the TARP deep tunnel system that flows directly under the North Branch of the Chicago River. Potential overflows coming from the upstream pipe and interceptor system are directed into the deep tunnel through sluice gates. System decision variables consist of sluice gate positions that control whether water enters the deep tunnel or flows into the river, as well as a treatment plant pumping rate on the interceptor lines. For different optimization runs, modifications are made to existing hydraulic structures to evaluate solution robustness for comparable hydraulic systems. The operational objective is to minimize the total volume of overflows for each storm event through a decision sequence, generated at discretized intervals, within a model predictive control (MPC) framework. Current operational strategies restrict water entry to the deep tunnel when an averaged tunnel water level reaches 70 percent of the diameter to avoid hydraulic transients. The optimization routine implements constraints that

  12. Real-time MRI-guided hyperthermia treatment using a fast adaptive algorithm

    NASA Astrophysics Data System (ADS)

    Stakhursky, Vadim L.; Arabe, Omar; Cheng, Kung-Shan; MacFall, James; Maccarini, Paolo; Craciunescu, Oana; Dewhirst, Mark; Stauffer, Paul; Das, Shiva K.

    2009-04-01

    temperature in the tumor to integral temperature in normal tissue) by up to six-fold, compared to the first iteration. The integrated MR-HT treatment algorithm successfully steered the focus of heating into the desired target volume for both the simple homogeneous and the more challenging muscle equivalent phantom with tumor insert models of human extremity sarcomas after 16 and 2 iterations, correspondingly. The adaptive method for MR thermal image guided focal steering shows promise when tested in phantom experiments on a four-antenna phased array applicator.

  13. Task-oriented quality assessment and adaptation in real-time mission critical video streaming applications

    NASA Astrophysics Data System (ADS)

    Nightingale, James; Wang, Qi; Grecos, Christos

    2015-02-01

    In recent years video traffic has become the dominant application on the Internet with global year-on-year increases in video-oriented consumer services. Driven by improved bandwidth in both mobile and fixed networks, steadily reducing hardware costs and the development of new technologies, many existing and new classes of commercial and industrial video applications are now being upgraded or emerging. Some of the use cases for these applications include areas such as public and private security monitoring for loss prevention or intruder detection, industrial process monitoring and critical infrastructure monitoring. The use of video is becoming commonplace in defence, security, commercial, industrial, educational and health contexts. Towards optimal performances, the design or optimisation in each of these applications should be context aware and task oriented with the characteristics of the video stream (frame rate, spatial resolution, bandwidth etc.) chosen to match the use case requirements. For example, in the security domain, a task-oriented consideration may be that higher resolution video would be required to identify an intruder than to simply detect his presence. Whilst in the same case, contextual factors such as the requirement to transmit over a resource-limited wireless link, may impose constraints on the selection of optimum task-oriented parameters. This paper presents a novel, conceptually simple and easily implemented method of assessing video quality relative to its suitability for a particular task and dynamically adapting videos streams during transmission to ensure that the task can be successfully completed. Firstly we defined two principle classes of tasks: recognition tasks and event detection tasks. These task classes are further subdivided into a set of task-related profiles, each of which is associated with a set of taskoriented attributes (minimum spatial resolution, minimum frame rate etc.). For example, in the detection class

  14. Chemically unassisted phototherapy: dose effects via real-time optical monitoring of cancer cells

    NASA Astrophysics Data System (ADS)

    Landry, Sylvie; Keeler, Werden

    2010-03-01

    Ultraviolet (UV) light and short wavelength visible (VIS) light have been used to kill pathogens for many years. Although the adverse effects of UV radiation on living cells have been extensively studied using biochemical and biomolecular techniques, most of the light therapies used for medical treatment are chemically assisted (i.e., photodynamic therapy). However, the use of light alone could prove both cost and therapeutically effective as an alternative treatment modality for localized diseases. In this study, real-time oblique incidence reflection (OIR) microscopy and image analysis were used to visualize and quantify the effects of chemically unassisted light therapy on untagged live cancer cells in vitro. The incident radiation fluence (in mJ/cm^2) required to induce cell death was determined for selected quasi-monochromatic UV to VIS wavelengths ranging from 275nm to 460nm. A predictive mathematical equation quantifying the lethal fluence as a function of wavelength will be discussed.

  15. Optical-electronic system for real-time position control of roof's supporting structure

    NASA Astrophysics Data System (ADS)

    Mikheev, Sergey V.; Konyakhin, Igor A.; Barsukov, Oleg A.

    2015-05-01

    During the designing and operation of extensive engineering facilities is particularly important as the task of controlling the spatial position of elements of their design in real time. This task is often complicated by variable external conditions: fluctuations in ambient temperature, humidity, wind speed, level of background light, etc. In this article we discussed some solutions to solve this problem. We proposed an original method for solving the system of differential equations to calculate the coordinates of the objects. It's verified the possibility of designing the system of measurement of the deformation through the control of individual structural parts. We provide the physical simulation of the system for controlling the position of the roof of large buildings. Much attention was devoted to the experiments to confirm the theoretical results.

  16. Real time obscuration monitoring

    NASA Astrophysics Data System (ADS)

    Agricola, Koos

    2016-09-01

    Recently a real time particle deposition monitoring system is developed. After discussions with optical system engineers a new feature has been added. This enables the real time monitoring of obscuration of exposed optical components by counting the deposited particles and sizing the obscuration area of each particle. This way the Particle Obscuration Rate (POR) can be determined. The POR can be used to determine the risk of product contamination during exposure. The particle size distribution gives information on the type of potential particle sources. The deposition moments will indicate when these sources were present.

  17. Real-time experimental demonstrations of software reconfigurable optical OFDM transceivers utilizing DSP-based digital orthogonal filters for SDN PONs.

    PubMed

    Duan, X; Giddings, R P; Bolea, M; Ling, Y; Cao, B; Mansoor, S; Tang, J M

    2014-08-11

    Real-time optical OFDM (OOFDM) transceivers with on-line software-controllable channel reconfigurability and transmission performance adaptability are experimentally demonstrated, for the first time, utilizing Hilbert-pair-based 32-tap digital orthogonal filters implemented in FPGAs. By making use of an 8-bit DAC/ADC operating at 2GS/s, an oversampling factor of 2 and an EML intensity modulator, the demonstrated RF conversion-free transceiver supports end-to-end real-time simultaneous adaptive transmissions, within a 1GHz signal spectrum region, of a 2.03Gb/s in-phase OOFDM channel and a 1.41Gb/s quadrature-phase OOFDM channel over a 25km SSMF IMDD system. In addition, detailed experimental explorations are also undertaken of key physical mechanisms limiting the maximum achievable transmission performance, impacts of transceiver's channel multiplexing/demultiplexing operations on the system BER performance, and the feasibility of utilizing adaptive modulation to combat impairments associated with low-complexity digital filter designs. Furthermore, experimental results indicate that the transceiver incorporating a fixed digital orthogonal filter DSP architecture can be made transparent to various signal modulation formats up to 64-QAM.

  18. A Real-Time Capable Software-Defined Receiver Using GPU for Adaptive Anti-Jam GPS Sensors

    PubMed Central

    Seo, Jiwon; Chen, Yu-Hsuan; De Lorenzo, David S.; Lo, Sherman; Enge, Per; Akos, Dennis; Lee, Jiyun

    2011-01-01

    Due to their weak received signal power, Global Positioning System (GPS) signals are vulnerable to radio frequency interference. Adaptive beam and null steering of the gain pattern of a GPS antenna array can significantly increase the resistance of GPS sensors to signal interference and jamming. Since adaptive array processing requires intensive computational power, beamsteering GPS receivers were usually implemented using hardware such as field-programmable gate arrays (FPGAs). However, a software implementation using general-purpose processors is much more desirable because of its flexibility and cost effectiveness. This paper presents a GPS software-defined radio (SDR) with adaptive beamsteering capability for anti-jam applications. The GPS SDR design is based on an optimized desktop parallel processing architecture using a quad-core Central Processing Unit (CPU) coupled with a new generation Graphics Processing Unit (GPU) having massively parallel processors. This GPS SDR demonstrates sufficient computational capability to support a four-element antenna array and future GPS L5 signal processing in real time. After providing the details of our design and optimization schemes for future GPU-based GPS SDR developments, the jamming resistance of our GPS SDR under synthetic wideband jamming is presented. Since the GPS SDR uses commercial-off-the-shelf hardware and processors, it can be easily adopted in civil GPS applications requiring anti-jam capabilities. PMID:22164116

  19. A real-time capable software-defined receiver using GPU for adaptive anti-jam GPS sensors.

    PubMed

    Seo, Jiwon; Chen, Yu-Hsuan; De Lorenzo, David S; Lo, Sherman; Enge, Per; Akos, Dennis; Lee, Jiyun

    2011-01-01

    Due to their weak received signal power, Global Positioning System (GPS) signals are vulnerable to radio frequency interference. Adaptive beam and null steering of the gain pattern of a GPS antenna array can significantly increase the resistance of GPS sensors to signal interference and jamming. Since adaptive array processing requires intensive computational power, beamsteering GPS receivers were usually implemented using hardware such as field-programmable gate arrays (FPGAs). However, a software implementation using general-purpose processors is much more desirable because of its flexibility and cost effectiveness. This paper presents a GPS software-defined radio (SDR) with adaptive beamsteering capability for anti-jam applications. The GPS SDR design is based on an optimized desktop parallel processing architecture using a quad-core Central Processing Unit (CPU) coupled with a new generation Graphics Processing Unit (GPU) having massively parallel processors. This GPS SDR demonstrates sufficient computational capability to support a four-element antenna array and future GPS L5 signal processing in real time. After providing the details of our design and optimization schemes for future GPU-based GPS SDR developments, the jamming resistance of our GPS SDR under synthetic wideband jamming is presented. Since the GPS SDR uses commercial-off-the-shelf hardware and processors, it can be easily adopted in civil GPS applications requiring anti-jam capabilities.

  20. Results from GROCSE I: A real-time search for gamma ray burst optical counterparts

    SciTech Connect

    Lee, B.; Akerlof, C.; Ables, E.

    1995-10-27

    The GROCSE I experiment (Gamma-Ray Optical Counterpart Search Experiment) is a rapid slewing wide field of view optical telescope at Lawrence Livermore National Laboratory which responds to triggers from the BATSE GRB data telemetry stream that have been processed and distributed by the BACODINE network. GROCSE 1 has been in continuous automated operation since January 1994. As of October 1995, sky images for 22 GRB triggers have been recorded, in some cases while the burst was still emitting gamma rays. The preliminary analysis of eight of these events are presented here. No optical counterparts have yet been detected. Limits for optical emission are given.

  1. Optical eye tracking system for real-time noninvasive tumor localization in external beam radiotherapy

    SciTech Connect

    Via, Riccardo Fassi, Aurora; Fattori, Giovanni; Fontana, Giulia; Pella, Andrea; Tagaste, Barbara; Ciocca, Mario; Riboldi, Marco; Baroni, Guido; Orecchia, Roberto

    2015-05-15

    Purpose: External beam radiotherapy currently represents an important therapeutic strategy for the treatment of intraocular tumors. Accurate target localization and efficient compensation of involuntary eye movements are crucial to avoid deviations in dose distribution with respect to the treatment plan. This paper describes an eye tracking system (ETS) based on noninvasive infrared video imaging. The system was designed for capturing the tridimensional (3D) ocular motion and provides an on-line estimation of intraocular lesions position based on a priori knowledge coming from volumetric imaging. Methods: Eye tracking is performed by localizing cornea and pupil centers on stereo images captured by two calibrated video cameras, exploiting eye reflections produced by infrared illumination. Additionally, torsional eye movements are detected by template matching in the iris region of eye images. This information allows estimating the 3D position and orientation of the eye by means of an eye local reference system. By combining ETS measurements with volumetric imaging for treatment planning [computed tomography (CT) and magnetic resonance (MR)], one is able to map the position of the lesion to be treated in local eye coordinates, thus enabling real-time tumor referencing during treatment setup and irradiation. Experimental tests on an eye phantom and seven healthy subjects were performed to assess ETS tracking accuracy. Results: Measurements on phantom showed an overall median accuracy within 0.16 mm and 0.40° for translations and rotations, respectively. Torsional movements were affected by 0.28° median uncertainty. On healthy subjects, the gaze direction error ranged between 0.19° and 0.82° at a median working distance of 29 cm. The median processing time of the eye tracking algorithm was 18.60 ms, thus allowing eye monitoring up to 50 Hz. Conclusions: A noninvasive ETS prototype was designed to perform real-time target localization and eye movement monitoring

  2. MTF measurements on real time for performance analysis of electro-optical systems

    NASA Astrophysics Data System (ADS)

    Stuchi, Jose Augusto; Signoreto Barbarini, Elisa; Vieira, Flavio Pascoal; dos Santos, Daniel, Jr.; Stefani, Mário Antonio; Yasuoka, Fatima Maria Mitsue; Castro Neto, Jarbas C.; Linhari Rodrigues, Evandro Luis

    2012-06-01

    The need of methods and tools that assist in determining the performance of optical systems is actually increasing. One of the most used methods to perform analysis of optical systems is to measure the Modulation Transfer Function (MTF). The MTF represents a direct and quantitative verification of the image quality. This paper presents the implementation of the software, in order to calculate the MTF of electro-optical systems. The software was used for calculating the MTF of Digital Fundus Camera, Thermal Imager and Ophthalmologic Surgery Microscope. The MTF information aids the analysis of alignment and measurement of optical quality, and also defines the limit resolution of optical systems. The results obtained with the Fundus Camera and Thermal Imager was compared with the theoretical values. For the Microscope, the results were compared with MTF measured of Microscope Zeiss model, which is the quality standard of ophthalmological microscope.

  3. The use of real-time optical feedback to improve outcomes

    NASA Astrophysics Data System (ADS)

    Magaña, Isidro B.; Adhikari, Pratik; Yendluri, Raghuvara B.; Goodrich, Glenn P.; Schwartz, Jon A.; O'Neal, D. P.

    2014-03-01

    More than a decade into the development of gold nanoparticles for cancer therapies, with multiple clinical trials underway, ongoing pre-clinical research continues towards better understanding in vivo interactions with the goal of treatment optimization through improved best practices. In an effort to collect information for healthcare providers, enabling informed decisions in a relevant time frame, instrumentation for real-time plasma concentration (multi-wavelength pulse photometry) and protocols for rapid elemental analysis (energy dispersive X-Ray fluorescence) of biopsied tumor tissue have been developed in a murine model. An initial analysis, designed to demonstrate the robust nature and utility of the techniques, revealed that area under the bioavailability curve (AUC) alone does not currently inform tumor accumulation with a high degree of accuracy (R2=0.32), This finding suggests that the control of additional experimental and physiological variables may yield more predictable tumor accumulation. Subject core temperature are blood pressure were monitored, but did not demonstrate clear trends. An effort to modulate AUC has produced an adjuvant therapy which is employed to enhance circulation parameters, including the AUC, of nanorods and gold nanoshells. Preliminary studies demonstrated a greater than 300% increase in average AUC through the use of a reticuloendothelial blockade agent versus control groups. Given a better understanding of the relative importance of the physiological factors which impact rates of tumor accumulation, a proposed set of experimental best practices is presented.

  4. Real time corner detection for miniaturized electro-optical sensors onboard small unmanned aerial systems.

    PubMed

    Forlenza, Lidia; Carton, Patrick; Accardo, Domenico; Fasano, Giancarmine; Moccia, Antonio

    2012-01-01

    This paper describes the target detection algorithm for the image processor of a vision-based system that is installed onboard an unmanned helicopter. It has been developed in the framework of a project of the French national aerospace research center Office National d'Etudes et de Recherches Aérospatiales (ONERA) which aims at developing an air-to-ground target tracking mission in an unknown urban environment. In particular, the image processor must detect targets and estimate ground motion in proximity of the detected target position. Concerning the target detection function, the analysis has dealt with realizing a corner detection algorithm and selecting the best choices in terms of edge detection methods, filtering size and type and the more suitable criterion of detection of the points of interest in order to obtain a very fast algorithm which fulfills the computation load requirements. The compared criteria are the Harris-Stephen and the Shi-Tomasi, ones, which are the most widely used in literature among those based on intensity. Experimental results which illustrate the performance of the developed algorithm and demonstrate that the detection time is fully compliant with the requirements of the real-time system are discussed.

  5. Real Time Corner Detection for Miniaturized Electro-Optical Sensors Onboard Small Unmanned Aerial Systems

    PubMed Central

    Forlenza, Lidia; Carton, Patrick; Accardo, Domenico; Fasano, Giancarmine; Moccia, Antonio

    2012-01-01

    This paper describes the target detection algorithm for the image processor of a vision-based system that is installed onboard an unmanned helicopter. It has been developed in the framework of a project of the French national aerospace research center Office National d’Etudes et de Recherches Aérospatiales (ONERA) which aims at developing an air-to-ground target tracking mission in an unknown urban environment. In particular, the image processor must detect targets and estimate ground motion in proximity of the detected target position. Concerning the target detection function, the analysis has dealt with realizing a corner detection algorithm and selecting the best choices in terms of edge detection methods, filtering size and type and the more suitable criterion of detection of the points of interest in order to obtain a very fast algorithm which fulfills the computation load requirements. The compared criteria are the Harris-Stephen and the Shi-Tomasi, ones, which are the most widely used in literature among those based on intensity. Experimental results which illustrate the performance of the developed algorithm and demonstrate that the detection time is fully compliant with the requirements of the real-time system are discussed. PMID:22368499

  6. Experimental demonstration of the real-time online fault monitoring technique for chaos-based passive optical networks

    NASA Astrophysics Data System (ADS)

    Dou, Xinyu; Yin, Hongxi; Yue, Hehe; Jin, Yu; Shen, Jing; Li, Lin

    2015-09-01

    In this paper, a real-time online fault monitoring technique for chaos-based passive optical networks (PONs) is proposed and experimentally demonstrated. The fault monitoring is performed by the chaotic communication signal. The proof-of-concept experiments are demonstrated for two PON structures, i.e., wavelength-division-multiplexing (WDM) PON and Ethernet PON (EPON), respectively. For WDM PON, two monitoring approaches are investigated, one deploying a chaotic optical time domain reflectometry (OTDR) for each transmitter, and the other using only one tunable chaotic OTDR. The experimental results show that the faults at beyond 20 km from the OLT can be detected and located. The spatial resolution of the tunable chaotic OTDR is an order of magnitude of centimeter. Meanwhile, the monitoring process can operate in parallel with the chaotic optical secure communications. The proposed technique has benefits of real-time, online, precise fault location, and simple realization, which will significantly reduce the cost of operation, administration and maintenance (OAM) of PON.

  7. How much can history constrain adaptive evolution? A real-time evolutionary approach of inversion polymorphisms in Drosophila subobscura.

    PubMed

    Fragata, I; Lopes-Cunha, M; Bárbaro, M; Kellen, B; Lima, M; Santos, M A; Faria, G S; Santos, M; Matos, M; Simões, P

    2014-12-01

    Chromosomal inversions are present in a wide range of animals and plants, having an important role in adaptation and speciation. Although empirical evidence of their adaptive value is abundant, the role of different processes underlying evolution of chromosomal polymorphisms is not fully understood. History and selection are likely to shape inversion polymorphism variation to an extent yet largely unknown. Here, we perform a real-time evolution study addressing the role of historical constraints and selection in the evolution of these polymorphisms. We founded laboratory populations of Drosophila subobscura derived from three locations along the European cline and followed the evolutionary dynamics of inversion polymorphisms throughout the first 40 generations. At the beginning, populations were highly differentiated and remained so throughout generations. We report evidence of positive selection for some inversions, variable between foundations. Signs of negative selection were more frequent, in particular for most cold-climate standard inversions across the three foundations. We found that previously observed convergence at the phenotypic level in these populations was not associated with convergence in inversion frequencies. In conclusion, our study shows that selection has shaped the evolutionary dynamics of inversion frequencies, but doing so within the constraints imposed by previous history. Both history and selection are therefore fundamental to predict the evolutionary potential of different populations to respond to global environmental changes.

  8. A Feature-adaptive Subdivision Method for Real-time 3D Reconstruction of Repeated Topology Surfaces

    NASA Astrophysics Data System (ADS)

    Lin, Jinhua; Wang, Yanjie; Sun, Honghai

    2017-03-01

    It's well known that rendering for a large number of triangles with GPU hardware tessellation has made great progress. However, due to the fixed nature of GPU pipeline, many off-line methods that perform well can not meet the on-line requirements. In this paper, an optimized Feature-adaptive subdivision method is proposed, which is more suitable for reconstructing surfaces with repeated cusps or creases. An Octree primitive is established in irregular regions where there are the same sharp vertices or creases, this method can find the neighbor geometry information quickly. Because of having the same topology structure between Octree primitive and feature region, the Octree feature points can match the arbitrary vertices in feature region more precisely. In the meanwhile, the patches is re-encoded in the Octree primitive by using the breadth-first strategy, resulting in a meta-table which allows for real-time reconstruction by GPU hardware tessellation unit. There is only one feature region needed to be calculated under Octree primitive, other regions with the same repeated feature generate their own meta-table directly, the reconstruction time is saved greatly for this step. With regard to the meshes having a large number of repeated topology feature, our algorithm improves the subdivision time by 17.575% and increases the average frame drawing time by 0.2373 ms compared to the traditional FAS (Feature-adaptive Subdivision), at the same time the model can be reconstructed in a watertight manner.

  9. Development of photorefractive polymers for real-time optical information storage

    NASA Astrophysics Data System (ADS)

    Mangaiyarkarasi, D.; Palanisamy, P. K.; Kannan, P.

    1999-03-01

    A large number of strong nonlinear optical and electro optical molecules and crystals are identified recently. With the discovery of the photorefractive (PR) effect and early realization of its potential utility, PR materials are of considerable interest for the development of all optical devices, such as high density optical data storage and image processing techniques. Organic materials are known to show strong electro optic effects. In organic materials, the properties required for the PR effect including photosensitivity, photoconductivity and electro optic response are provided by different molecules. As a result, the properties can be optimized separately, unlike in inorganic PR crystals such as LiNbO3. This paper describes the utilization of third order non-linearity induced in Xanthene dye doped gelatin and poly (eosin acrylate) & poly (eosin acrylate-co-isobutyl acrylate) films resulting in direct storage without the need for any further processing i.e., no wet chemical or post thermal/photochemical processing are required. With required amount of solvent and monomer in the presence of benzoyl peroxide (initiator), polymerization reaction was carried out under nitrogen atmosphere. Polymer samples were characterized by NMR, IR, FT-IR & TGA. The polymers were soluble in THF, DMSO, DMF & DMAC solvents and form good optical quality films by spin as well as dip coating. Polymer thin films were prepared with different concentrations of polymer solution onto the glass slides. The UV-visible absorption spectra of the spin coated polymer films showed a maximum at 538 nm. In our simplest optical system, Q- switched, second harmonic Nd-YAG laser light at wavelength 532 nm was used for recording. Two beams split from the same laser were made to superpose with path difference less than the coherent length. One of the beam acted as information carrying beam while the other acted as reference beam. In this present study, we report the direction formation of surface

  10. Real-Time Temperature Measurement in a Vacuum Degasser Using Optical Pyrometry

    SciTech Connect

    Michel Bonin - Process Metrix; Peter Hues - Process Metrix; William Federoff - US Steel Nichloas Rymarchyk - Berry Metals; Todd Smith - Berry Metals

    2007-02-14

    The objective of the research was the development of a fiber-coupled, optical pyrometer for continuous temperature measurement in a vacuum degasser that reduces process time, enhances process control, and eliminates manual or robot-operated thermocouples. Through the live testing performed at US Steel's Edgar Thompson Works, the challenges associated with making optical temperature measurements in a vacuum chamber were identified. As a result of these challenges it was determined that continuous temperature monitoring in RH-type degassers was not a viable alternative to standard immersion thermocouples. The project was not successful.

  11. Real-time optical reflectometry enabled by amplified dispersive Fourier transformation

    NASA Astrophysics Data System (ADS)

    Goda, Keisuke; Solli, Daniel R.; Jalali, Bahram

    2008-07-01

    The axial scan rate of optical frequency-domain reflectometry and optical coherence tomography can be increased to megahertz frequencies by dispersive Fourier transformation. However, the fundamental connection between dispersion and loss creates a trade-off between detection sensitivity and acquisition speed. Here we circumvent this predicament by using distributed Raman postamplification of the reflection from the sample. The Raman amplification enables measurement of weak signals, which are otherwise buried in detector noise. It extends the depth range without sacrificing the acquisition speed. Single-shot imaging with improved sensitivity at an axial scan rate of 36.6MHz is demonstrated.

  12. Rugged, Portable, Real-Time Optical Gaseous Analyzer for Hydrogen Fluoride

    NASA Technical Reports Server (NTRS)

    Pilgrim, Jeffrey; Gonzales, Paula

    2012-01-01

    Hydrogen fluoride (HF) is a primary evolved combustion product of fluorinated and perfluorinated hydrocarbons. HF is produced during combustion by the presence of impurities and hydrogen- containing polymers including polyimides. This effect is especially dangerous in closed occupied volumes like spacecraft and submarines. In these systems, combinations of perfluorinated hydrocarbons and polyimides are used for insulating wiring. HF is both highly toxic and short-lived in closed environments due to its reactivity. The high reactivity also makes HF sampling problematic. An infrared optical sensor can detect promptly evolving HF with minimal sampling requirements, while providing both high sensitivity and high specificity. A rugged optical path length enhancement architecture enables both high HF sensitivity and rapid environmental sampling with minimal gaseous contact with the low-reactivity sensor surfaces. The inert optical sample cell, combined with infrared semiconductor lasers, is joined with an analog and digital electronic control architecture that allows for ruggedness and compactness. The combination provides both portability and battery operation on a simple camcorder battery for up to eight hours. Optical detection of gaseous HF is confounded by the need for rapid sampling with minimal contact between the sensor and the environmental sample. A sensor is required that must simultaneously provide the required sub-parts-permillion detection limits, but with the high specificity and selectivity expected of optical absorption techniques. It should also be rugged and compact for compatibility with operation onboard spacecraft and submarines. A new optical cell has been developed for which environmental sampling is accomplished by simply traversing the few mm-thick cell walls into an open volume where the measurement is made. A small, low-power fan or vacuum pump may be used to push or pull the gaseous sample into the sample volume for a response time of a few

  13. Porous Silicon-Based Biosensors: Towards Real-Time Optical Detection of Target Bacteria in the Food Industry.

    PubMed

    Massad-Ivanir, Naama; Shtenberg, Giorgi; Raz, Nitzan; Gazenbeek, Christel; Budding, Dries; Bos, Martine P; Segal, Ester

    2016-11-30

    Rapid detection of target bacteria is crucial to provide a safe food supply and to prevent foodborne diseases. Herein, we present an optical biosensor for identification and quantification of Escherichia coli (E. coli, used as a model indicator bacteria species) in complex food industry process water. The biosensor is based on a nanostructured, oxidized porous silicon (PSi) thin film which is functionalized with specific antibodies against E. coli. The biosensors were exposed to water samples collected directly from process lines of fresh-cut produce and their reflectivity spectra were collected in real time. Process water were characterized by complex natural micro-flora (microbial load of >10(7) cell/mL), in addition to soil particles and plant cell debris. We show that process water spiked with culture-grown E. coli, induces robust and predictable changes in the thin-film optical interference spectrum of the biosensor. The latter is ascribed to highly specific capture of the target cells onto the biosensor surface, as confirmed by real-time polymerase chain reaction (PCR). The biosensors were capable of selectively identifying and quantifying the target cells, while the target cell concentration is orders of magnitude lower than that of other bacterial species, without any pre-enrichment or prior processing steps.

  14. Porous Silicon-Based Biosensors: Towards Real-Time Optical Detection of Target Bacteria in the Food Industry

    PubMed Central

    Massad-Ivanir, Naama; Shtenberg, Giorgi; Raz, Nitzan; Gazenbeek, Christel; Budding, Dries; Bos, Martine P.; Segal, Ester

    2016-01-01

    Rapid detection of target bacteria is crucial to provide a safe food supply and to prevent foodborne diseases. Herein, we present an optical biosensor for identification and quantification of Escherichia coli (E. coli, used as a model indicator bacteria species) in complex food industry process water. The biosensor is based on a nanostructured, oxidized porous silicon (PSi) thin film which is functionalized with specific antibodies against E. coli. The biosensors were exposed to water samples collected directly from process lines of fresh-cut produce and their reflectivity spectra were collected in real time. Process water were characterized by complex natural micro-flora (microbial load of >107 cell/mL), in addition to soil particles and plant cell debris. We show that process water spiked with culture-grown E. coli, induces robust and predictable changes in the thin-film optical interference spectrum of the biosensor. The latter is ascribed to highly specific capture of the target cells onto the biosensor surface, as confirmed by real-time polymerase chain reaction (PCR). The biosensors were capable of selectively identifying and quantifying the target cells, while the target cell concentration is orders of magnitude lower than that of other bacterial species, without any pre-enrichment or prior processing steps. PMID:27901131

  15. Porous Silicon-Based Biosensors: Towards Real-Time Optical Detection of Target Bacteria in the Food Industry

    NASA Astrophysics Data System (ADS)

    Massad-Ivanir, Naama; Shtenberg, Giorgi; Raz, Nitzan; Gazenbeek, Christel; Budding, Dries; Bos, Martine P.; Segal, Ester

    2016-11-01

    Rapid detection of target bacteria is crucial to provide a safe food supply and to prevent foodborne diseases. Herein, we present an optical biosensor for identification and quantification of Escherichia coli (E. coli, used as a model indicator bacteria species) in complex food industry process water. The biosensor is based on a nanostructured, oxidized porous silicon (PSi) thin film which is functionalized with specific antibodies against E. coli. The biosensors were exposed to water samples collected directly from process lines of fresh-cut produce and their reflectivity spectra were collected in real time. Process water were characterized by complex natural micro-flora (microbial load of >107 cell/mL), in addition to soil particles and plant cell debris. We show that process water spiked with culture-grown E. coli, induces robust and predictable changes in the thin-film optical interference spectrum of the biosensor. The latter is ascribed to highly specific capture of the target cells onto the biosensor surface, as confirmed by real-time polymerase chain reaction (PCR). The biosensors were capable of selectively identifying and quantifying the target cells, while the target cell concentration is orders of magnitude lower than that of other bacterial species, without any pre-enrichment or prior processing steps.

  16. Real time measurements of PM2.5 concentrations and vertical turbulent fluxes using an optical detector

    NASA Astrophysics Data System (ADS)

    Donateo, Antonio; Contini, Daniele; Belosi, Franco

    In this work the possibility of measuring real-time concentrations of PM2.5 and the corresponding vertical turbulent fluxes using the optical detector Mie pDR-1200, operating synchronously with an ultrasonic anemometer, is investigated. This detector is known to be sensitive to high values of relative humidity (RH) and a new procedure to correct the effect of RH on concentration measurements is presented. Results of optical measurements have been compared with gravimetric detections of PM2.5 and results show a reasonable correlation between them and an improvement of the agreement when RH-correction is used. Results presented have been collected at two measurement sites that can be representative of urban background environments but in one of them was present an industrial area nearby. Post-processing of data has been performed with the eddy-correlation technique that allows evaluation of vertical turbulent fluxes of PM2.5 as well as sensible heat and momentum fluxes. The turbulent mass fluxes, together with the analysis of real-time concentrations and their correlation with meteorology proved to be an useful tool to infer details about the local aerosol dynamics helping to interpret traditional gravimetric analysis of aerosol that is usually performed on a 24 h basis. Results show that the methodology can be useful in identifying the contribution of local sources like ground level emissions or industrial plumes with respect to the contribution of sources located far away from the measurement site.

  17. Real-Time Correction By Optical Tracking with Integrated Geometric Distortion Correction for Reducing Motion Artifacts in fMRI

    NASA Astrophysics Data System (ADS)

    Rotenberg, David J.

    Artifacts caused by head motion are a substantial source of error in fMRI that limits its use in neuroscience research and clinical settings. Real-time scan-plane correction by optical tracking has been shown to correct slice misalignment and non-linear spin-history artifacts, however residual artifacts due to dynamic magnetic field non-uniformity may remain in the data. A recently developed correction technique, PLACE, can correct for absolute geometric distortion using the complex image data from two EPI images, with slightly shifted k-space trajectories. We present a correction approach that integrates PLACE into a real-time scan-plane update system by optical tracking, applied to a tissue-equivalent phantom undergoing complex motion and an fMRI finger tapping experiment with overt head motion to induce dynamic field non-uniformity. Experiments suggest that including volume by volume geometric distortion correction by PLACE can suppress dynamic geometric distortion artifacts in a phantom and in vivo and provide more robust activation maps.

  18. ERIS adaptive optics system design

    NASA Astrophysics Data System (ADS)

    Marchetti, Enrico; Le Louarn, Miska; Soenke, Christian; Fedrigo, Enrico; Madec, Pierre-Yves; Hubin, Norbert

    2012-07-01

    The Enhanced Resolution Imager and Spectrograph (ERIS) is the next-generation instrument planned for the Very Large Telescope (VLT) and the Adaptive Optics facility (AOF). It is an AO assisted instrument that will make use of the Deformable Secondary Mirror and the new Laser Guide Star Facility (4LGSF), and it is planned for the Cassegrain focus of the telescope UT4. The project is currently in its Phase A awaiting for approval to continue to the next phases. The Adaptive Optics system of ERIS will include two wavefront sensors (WFS) to maximize the coverage of the proposed sciences cases. The first is a high order 40x40 Pyramid WFS (PWFS) for on axis Natural Guide Star (NGS) observations. The second is a high order 40x40 Shack-Hartmann WFS for single Laser Guide Stars (LGS) observations. The PWFS, with appropriate sub-aperture binning, will serve also as low order NGS WFS in support to the LGS mode with a field of view patrolling capability of 2 arcmin diameter. Both WFSs will be equipped with the very low read-out noise CCD220 based camera developed for the AOF. The real-time reconstruction and control is provided by a SPARTA real-time platform adapted to support both WFS modes. In this paper we will present the ERIS AO system in all its main aspects: opto-mechanical design, real-time computer design, control and calibrations strategy. Particular emphasis will be given to the system performance obtained via dedicated numerical simulations.

  19. Real-time optical fiber digital speckle pattern interferometry for industrial applications

    NASA Astrophysics Data System (ADS)

    Chan, Robert K.; Cheung, Y. M.; Lo, C. H.; Tam, T. K.

    1997-03-01

    There is current interest, especially in the industrial sector, to use the digital speckle pattern interferometry (DSPI) technique to measure surface stress. Indeed, many publications in the subject are evident of the growing interests in the field. However, to bring the technology to industrial use requires the integration of several emerging technologies, viz. optics, feedback control, electronics, imaging processing and digital signal processing. Due to the highly interdisciplinary nature of the technique, successful implementation and development require expertise in all of the fields. At Baptist University, under the funding of a major industrial grant, we are developing the technology for the industrial sector. Our system fully exploits optical fibers and diode lasers in the design to enable practical and rugged systems suited for industrial applications. Besides the development in optics, we have broken away from the reliance of a microcomputer PC platform for both image capture and processing, and have developed a digital signal processing array system that can handle simultaneous and independent image capture/processing with feedback control. The system, named CASPA for 'cascadable architecture signal processing array,' is a third generation development system that utilizes up to 7 digital signal processors has proved to be a very powerful system. With our CASPA we are now in a better position to developing novel optical measurement systems for industrial application that may require different measurement systems to operate concurrently and requiring information exchange between the systems. Applications in mind such as simultaneous in-plane and out-of-plane DSPI image capture/process, vibrational analysis with interactive DSPI and phase shifting control of optical systems are a few good examples of the potentials.

  20. Effective learning strategies for real-time image-guided adaptive control of multiple-source hyperthermia applicators

    PubMed Central

    Cheng, Kung-Shan; Dewhirst, Mark W.; Stauffer, Paul R.; Das, Shiva

    2010-01-01

    Purpose: This paper investigates overall theoretical requirements for reducing the times required for the iterative learning of a real-time image-guided adaptive control routine for multiple-source heat applicators, as used in hyperthermia and thermal ablative therapy for cancer. Methods: Methods for partial reconstruction of the physical system with and without model reduction to find solutions within a clinically practical timeframe were analyzed. A mathematical analysis based on the Fredholm alternative theorem (FAT) was used to compactly analyze the existence and uniqueness of the optimal heating vector under two fundamental situations: (1) noiseless partial reconstruction and (2) noisy partial reconstruction. These results were coupled with a method for further acceleration of the solution using virtual source (VS) model reduction. The matrix approximation theorem (MAT) was used to choose the optimal vectors spanning the reduced-order subspace to reduce the time for system reconstruction and to determine the associated approximation error. Numerical simulations of the adaptive control of hyperthermia using VS were also performed to test the predictions derived from the theoretical analysis. A thigh sarcoma patient model surrounded by a ten-antenna phased-array applicator was retained for this purpose. The impacts of the convective cooling from blood flow and the presence of sudden increase of perfusion in muscle and tumor were also simulated. Results: By FAT, partial system reconstruction directly conducted in the full space of the physical variables such as phases and magnitudes of the heat sources cannot guarantee reconstructing the optimal system to determine the global optimal setting of the heat sources. A remedy for this limitation is to conduct the partial reconstruction within a reduced-order subspace spanned by the first few maximum eigenvectors of the true system matrix. By MAT, this VS subspace is the optimal one when the goal is to maximize the

  1. Real-time verification of multileaf collimator-driven radiotherapy using a novel optical attenuation-based fluence monitor

    SciTech Connect

    Goulet, Mathieu; Gingras, Luc; Beaulieu, Luc

    2011-03-15

    observed a very low intrinsic dispersion, dominated by Poisson statistics, for both x{sub c} (standard deviations of less than 1 mm) and {Phi}{sub int} (standard deviations of less than 0.20%). When confronted with random leaf positioning errors from IMRT segments, {Phi}{sub int} was highly sensitive to single leaf positioning errors as small as 1 mm at isocenter, while x{sub c} was sensitive to leaf pair translation errors of at least 2 mm at isocenter. Owing to the uncertainties in the doses calculated in regions of high perpendicular dose gradients, the measured values of x{sub c} and {Phi}{sub int} deviated from the predicted values of x{sub c} and {Phi}{sub int} by a mean of 1.3 mm and 2.6%, respectively. Conclusion: Our study showed that an optical attenuation-based detector can be used to effectively monitor integral fluence during radiotherapy delivery. The performance of such a system would enable real-time quality control of the incident fluence in current MLC-driven treatments such as IMRT and future adaptive radiotherapy procedures where new treatment plans will have to be delivered without passing thru the current standard quality control chain.

  2. Studying taxis in real time using optical tweezers: applications for Leishmania amazonensis parasites.

    PubMed

    Pozzo, L Y; Fontes, A; de Thomaz, A A; Santos, B S; Farias, P M A; Ayres, D C; Giorgio, S; Cesar, C L

    2009-01-01

    Beads trapped by an optical tweezers can be used as a force transducer for measuring forces of the same order of magnitude as typical forces induced by flagellar motion. We used an optical tweezers to study chemotaxis by observing the force response of a flagellated microorganism when placed in a gradient of attractive chemical substances. This report shows such observations for Leishmania amazonensis, responsible for leishmaniasis, a serious disease. We quantified the movement of this protozoan for different gradients of glucose. We were able to observe both the strength and the directionality of the force. The characterization of the chemotaxis of these parasites can help to understand the mechanics of infection and improve the treatments employed for this disease. This methodology can be used to quantitatively study the taxis of any kind of flagellated microorganisms under concentration gradients of different chemical substances, or even other types of variable gradients such as temperature and pressure.

  3. Structural and optical nanoengineering of nanoporous anodic alumina rugate filters for real-time and label-free biosensing applications.

    PubMed

    Kumeria, Tushar; Rahman, Mohammad Mahbubur; Santos, Abel; Ferré-Borrull, Josep; Marsal, Lluís F; Losic, Dusan

    2014-02-04

    In this study, we report about the structural engineering and optical optimization of nanoporous anodic alumina rugate filters (NAA-RFs) for real-time and label-free biosensing applications. Structurally engineered NAA-RFs are combined with reflection spectroscopy (RfS) in order to develop a biosensing system based on the position shift of the characteristic peak in the reflection spectrum of NAA-RFs (Δλpeak). This system is optimized and assessed by measuring shifts in the characteristic peak position produced by small changes in the effective medium (i.e., refractive index). To this end, NAA-RFs are filled with different solutions of d-glucose, and the Δλpeak is measured in real time by RfS. These results are validated by a theoretical model (i.e., the Looyenga-Landau-Lifshitz model), demonstrating that the control over the nanoporous structure makes it possible to optimize optical signals in RfS for sensing purposes. The linear range of these optical sensors ranges from 0.01 to 1.00 M, with a low detection limit of 0.01 M of d-glucose (i.e., 1.80 ppm), a sensitivity of 4.93 nm M(-1) (i.e., 164 nm per refractive index units), and a linearity of 0.998. This proof-of-concept study demonstrates that the proposed system combining NAA-RFs with RfS has outstanding capabilities to develop ultrasensitive, portable, and cost-competitive optical sensors.

  4. Towards Real-Time Maneuver Detection: Automatic State and Dynamics Estimation with the Adaptive Optimal Control Based Estimator

    NASA Astrophysics Data System (ADS)

    Lubey, D.; Scheeres, D.

    Tracking objects in Earth orbit is fraught with complications. This is due to the large population of orbiting spacecraft and debris that continues to grow, passive (i.e. no direct communication) and data-sparse observations, and the presence of maneuvers and dynamics mismodeling. Accurate orbit determination in this environment requires an algorithm to capture both a system's state and its state dynamics in order to account for mismodelings. Previous studies by the authors yielded an algorithm called the Optimal Control Based Estimator (OCBE) - an algorithm that simultaneously estimates a system's state and optimal control policies that represent dynamic mismodeling in the system for an arbitrary orbit-observer setup. The stochastic properties of these estimated controls are then used to determine the presence of mismodelings (maneuver detection), as well as characterize and reconstruct the mismodelings. The purpose of this paper is to develop the OCBE into an accurate real-time orbit tracking and maneuver detection algorithm by automating the algorithm and removing its linear assumptions. This results in a nonlinear adaptive estimator. In its original form the OCBE had a parameter called the assumed dynamic uncertainty, which is selected by the user with each new measurement to reflect the level of dynamic mismodeling in the system. This human-in-the-loop approach precludes real-time application to orbit tracking problems due to their complexity. This paper focuses on the Adaptive OCBE, a version of the estimator where the assumed dynamic uncertainty is chosen automatically with each new measurement using maneuver detection results to ensure that state uncertainties are properly adjusted to account for all dynamic mismodelings. The paper also focuses on a nonlinear implementation of the estimator. Originally, the OCBE was derived from a nonlinear cost function then linearized about a nominal trajectory, which is assumed to be ballistic (i.e. the nominal optimal

  5. An improved optical flow tracking technique for real-time MR-guided beam therapies in moving organs

    NASA Astrophysics Data System (ADS)

    Zachiu, C.; Papadakis, N.; Ries, M.; Moonen, C.; de Senneville, B. Denis

    2015-12-01

    Magnetic resonance (MR) guided high intensity focused ultrasound and external beam radiotherapy interventions, which we shall refer to as beam therapies/interventions, are promising techniques for the non-invasive ablation of tumours in abdominal organs. However, therapeutic energy delivery in these areas becomes challenging due to the continuous displacement of the organs with respiration. Previous studies have addressed this problem by coupling high-framerate MR-imaging with a tracking technique based on the algorithm proposed by Horn and Schunck (H and S), which was chosen due to its fast convergence rate and highly parallelisable numerical scheme. Such characteristics were shown to be indispensable for the real-time guidance of beam therapies. In its original form, however, the algorithm is sensitive to local grey-level intensity variations not attributed to motion such as those that occur, for example, in the proximity of pulsating arteries. In this study, an improved motion estimation strategy which reduces the impact of such effects is proposed. Displacements are estimated through the minimisation of a variation of the H and S functional for which the quadratic data fidelity term was replaced with a term based on the linear L1norm, resulting in what we have called an L2-L1 functional. The proposed method was tested in the livers and kidneys of two healthy volunteers under free-breathing conditions, on a data set comprising 3000 images equally divided between the volunteers. The results show that, compared to the existing approaches, our method demonstrates a greater robustness to local grey-level intensity variations introduced by arterial pulsations. Additionally, the computational time required by our implementation make it compatible with the work-flow of real-time MR-guided beam interventions. To the best of our knowledge this study was the first to analyse the behaviour of an L1-based optical flow functional in an applicative context: real-time MR

  6. Real-time, adaptive machine learning for non-stationary, near chaotic gasoline engine combustion time series.

    PubMed

    Vaughan, Adam; Bohac, Stanislav V

    2015-10-01

    Fuel efficient Homogeneous Charge Compression Ignition (HCCI) engine combustion timing predictions must contend with non-linear chemistry, non-linear physics, period doubling bifurcation(s), turbulent mixing, model parameters that can drift day-to-day, and air-fuel mixture state information that cannot typically be resolved on a cycle-to-cycle basis, especially during transients. In previous work, an abstract cycle-to-cycle mapping function coupled with ϵ-Support Vector Regression was shown to predict experimentally observed cycle-to-cycle combustion timing over a wide range of engine conditions, despite some of the aforementioned difficulties. The main limitation of the previous approach was that a partially acasual randomly sampled training dataset was used to train proof of concept offline predictions. The objective of this paper is to address this limitation by proposing a new online adaptive Extreme Learning Machine (ELM) extension named Weighted Ring-ELM. This extension enables fully causal combustion timing predictions at randomly chosen engine set points, and is shown to achieve results that are as good as or better than the previous offline method. The broader objective of this approach is to enable a new class of real-time model predictive control strategies for high variability HCCI and, ultimately, to bring HCCI's low engine-out NOx and reduced CO2 emissions to production engines.

  7. Neuromorphic learning of continuous-valued mappings from noise-corrupted data. Application to real-time adaptive control

    NASA Technical Reports Server (NTRS)

    Troudet, Terry; Merrill, Walter C.

    1990-01-01

    The ability of feed-forward neural network architectures to learn continuous valued mappings in the presence of noise was demonstrated in relation to parameter identification and real-time adaptive control applications. An error function was introduced to help optimize parameter values such as number of training iterations, observation time, sampling rate, and scaling of the control signal. The learning performance depended essentially on the degree of embodiment of the control law in the training data set and on the degree of uniformity of the probability distribution function of the data that are presented to the net during sequence. When a control law was corrupted by noise, the fluctuations of the training data biased the probability distribution function of the training data sequence. Only if the noise contamination is minimized and the degree of embodiment of the control law is maximized, can a neural net develop a good representation of the mapping and be used as a neurocontroller. A multilayer net was trained with back-error-propagation to control a cart-pole system for linear and nonlinear control laws in the presence of data processing noise and measurement noise. The neurocontroller exhibited noise-filtering properties and was found to operate more smoothly than the teacher in the presence of measurement noise.

  8. Neural adaptation and perceptual learning using a portable real-time cochlear implant simulator in natural environments.

    PubMed

    Smalt, Christopher J; Talavage, Thomas M; Pisoni, David B; Svirsky, Mario A

    2011-01-01

    A portable real-time speech processor that implements an acoustic simulation model of a cochlear implant (CI) has been developed on the Apple iPhone / iPod Touch to permit testing and experimentation under extended exposure in real-world environments. This simulator allows for both a variable number of noise band channels and electrode insertion depth. Utilizing this portable CI simulator, we tested perceptual learning in normal hearing listeners by measuring word and sentence comprehension behaviorally before and after 2 weeks of exposure. To evaluate changes in neural activation related to adaptation to transformed speech, fMRI was also conducted. Differences in brain activation after training occurred in the inferior frontal gyrus and areas related to language processing. A 15-20% improvement in word and sentence comprehension of cochlear implant simulated speech was also observed. These results demonstrate the effectiveness of a portable CI simulator as a research tool and provide new information about the physiological changes that accompany perceptual learning of degraded auditory input.

  9. Real-time optical multiple object recognition and tracking system and method

    NASA Technical Reports Server (NTRS)

    Chao, Tien-Hsin (Inventor); Liu, Hua-Kuang (Inventor)

    1990-01-01

    System for optically recognizing and tracking a plurality of objects within a field of vision. Laser (46) produces a coherent beam (48). Beam splitter (24) splits the beam into object (26) and reference (28) beams. Beam expanders (50) and collimators (52) transform the beams (26, 28) into coherent collimated light beams (26', 28'). A two-dimensional SLM (54), disposed in the object beam (26'), modulates the object beam with optical information as a function of signals from a first camera (16) which develops X and Y signals reflecting the contents of its field of vision. A hololens (38), positioned in the object beam (26') subsequent to the modulator (54), focuses the object beam at a plurality of focal points (42). A planar transparency-forming film (32), disposed with the focal points on an exposable surface, forms a multiple position interference filter (62) upon exposure of the surface and development processing of the film (32). A reflector (53) directing the reference beam (28') onto the film (32), exposes the surface, with images focused by the hololens (38), to form interference patterns on the surface. There is apparatus (16', 64) for sensing and indicating light passage through respective ones of the positions of the filter (62), whereby recognition of objects corresponding to respective ones of the positions of the filter (62) is affected. For tracking, apparatus (64) focuses light passing through the filter (62) onto a matrix of CCD's in a second camera (16') to form a two-dimensional display of the recognized objects.

  10. Fiber optic distributed chemical sensor for the real time detection of hydrocarbon fuel leaks

    NASA Astrophysics Data System (ADS)

    Mendoza, Edgar; Kempen, C.; Esterkin, Yan; Sun, Sunjian

    2015-09-01

    With the increase worldwide demand for hydrocarbon fuels and the vast development of new fuel production and delivery infrastructure installations around the world, there is a growing need for reliable hydrocarbon fuel leak detection technologies to provide safety and reduce environmental risks. Hydrocarbon leaks (gas or liquid) pose an extreme danger and need to be detected very quickly to avoid potential disasters. Gas leaks have the greatest potential for causing damage due to the explosion risk from the dispersion of gas clouds. This paper describes progress towards the development of a fast response, high sensitivity, distributed fiber optic fuel leak detection (HySense™) system based on the use of an optical fiber that uses a hydrocarbon sensitive fluorescent coating to detect the presence of fuel leaks present in close proximity along the length of the sensor fiber. The HySense™ system operates in two modes, leak detection and leak localization, and will trigger an alarm within seconds of exposure contact. The fast and accurate response of the sensor provides reliable fluid leak detection for pipelines, storage tanks, airports, pumps, and valves to detect and minimize any potential catastrophic damage.

  11. Real-Time Monitoring of Morphology and Optical Properties during Sputter Deposition for Tailoring Metal-Polymer Interfaces.

    PubMed

    Schwartzkopf, Matthias; Santoro, Gonzalo; Brett, Calvin J; Rothkirch, André; Polonskyi, Oleksandr; Hinz, Alexander; Metwalli, Ezzeldin; Yao, Yuan; Strunskus, Thomas; Faupel, Franz; Müller-Buschbaum, Peter; Roth, Stephan V

    2015-06-24

    The reproducible low-cost fabrication of functional metal-polymer nanocomposites with tailored optoelectronic properties for advanced applications remains a major challenge in applied nanotechnology. To obtain full control over the nanostructural evolution at the metal-polymer interface and its impact on optoelectronic properties, we employed combined in situ time-resolved microfocus grazing incidence small angle X-ray scattering (μGISAXS) with in situ UV/vis specular reflectance spectroscopy (SRS) during sputter deposition of gold on thin polystyrene films. On the basis of the temporal evolution of the key scattering features in the real-time μGISAXS experiment, we directly observed four different growth regimes: nucleation, isolated island growth, growth of larger aggregates via partial coalescence, and continuous layer growth. Moreover, their individual thresholds were identified with subnanometer resolution and correlated to the changes in optical properties. During sputter deposition, a change in optical reflectivity of the pristine gray-blue PS film was observed ranging from dark blue color due to the presence of isolated nanoclusters at the interface to bright red color from larger Au aggregates. We used simplified geometrical assumptions to model the evolution of average real space parameters (distance, size, density, contact angle) in excellent agreement with the qualitative observation of key scattering features. A decrease of contact angles was observed during the island-to-percolation transition and confirmed by simulations. Furthermore, a surface diffusion coefficient according to the kinetic freezing model and interfacial energy of Au on PS at room temperature were calculated based on a real-time experiment. The morphological characterization is complemented by X-ray reflectivity, optical, and electron microscopy. Our study permits a better understanding of the growth kinetics of gold clusters and their self-organization into complex nanostructures

  12. Adaptive Radiation Therapy for Postprostatectomy Patients Using Real-Time Electromagnetic Target Motion Tracking During External Beam Radiation Therapy

    SciTech Connect

    Zhu, Mingyao; Bharat, Shyam; Michalski, Jeff M.; Gay, Hiram A.; Hou, Wei-Hsien; Parikh, Parag J.

    2013-03-15

    Purpose: Using real-time electromagnetic (EM) transponder tracking data recorded by the Calypso 4D Localization System, we report inter- and intrafractional target motion of the prostate bed, describe a strategy to evaluate treatment adequacy in postprostatectomy patients receiving intensity modulated radiation therapy (IMRT), and propose an adaptive workflow. Methods and Materials: Tracking data recorded by Calypso EM transponders was analyzed for postprostatectomy patients that underwent step-and-shoot IMRT. Rigid target motion parameters during beam delivery were calculated from recorded transponder positions in 16 patients with rigid transponder geometry. The delivered doses to the clinical target volume (CTV) were estimated from the planned dose matrix and the target motion for the first 3, 5, 10, and all fractions. Treatment adequacy was determined by comparing the delivered minimum dose (D{sub min}) with the planned D{sub min} to the CTV. Treatments were considered adequate if the delivered CTV D{sub min} is at least 95% of the planned CTV D{sub min}. Results: Translational target motion was minimal for all 16 patients (mean: 0.02 cm; range: −0.12 cm to 0.07 cm). Rotational motion was patient-specific, and maximum pitch, yaw, and roll were 12.2, 4.1, and 10.5°, respectively. We observed inadequate treatments in 5 patients. In these treatments, we observed greater target rotations along with large distances between the CTV centroid and transponder centroid. The treatment adequacy from the initial 10 fractions successfully predicted the overall adequacy in 4 of 5 inadequate treatments and 10 of 11 adequate treatments. Conclusion: Target rotational motion could cause underdosage to partial volume of the postprostatectomy targets. Our adaptive treatment strategy is applicable to post-prostatectomy patients receiving IMRT to evaluate and improve radiation therapy delivery.

  13. Multi-particle three-dimensional coordinate estimation in real-time optical manipulation

    NASA Astrophysics Data System (ADS)

    Dam, J. S.; Perch-Nielsen, I.; Palima, D.; Gluckstad, J.

    2009-11-01

    We have previously shown how stereoscopic images can be obtained in our three-dimensional optical micromanipulation system [J. S. Dam et al, Opt. Express 16, 7244 (2008)]. Here, we present an extension and application of this principle to automatically gather the three-dimensional coordinates for all trapped particles with high tracking range and high reliability without requiring user calibration. Through deconvolving of the red, green, and blue colour planes to correct for bleeding between colour planes, we show that we can extend the system to also utilize green illumination, in addition to the blue and red. Applying the green colour as on-axis illumination yields redundant information for enhanced error correction, which is used to verify the gathered data, resulting in reliable coordinates as well as producing visually attractive images.

  14. Real time optical edge enhancement using a Hughes liquid crystal light valve

    NASA Technical Reports Server (NTRS)

    Chao, Tien-Hsin

    1989-01-01

    The discovery of an edge enhancement effect in using a Hughes CdS liquid crystal light valve (LCLV) is reported. An edge-enhanced version of the input writing image can be directly obtained by operating the LCLV at a lower bias frequency and bias voltage. Experimental conditions in which this edge enhancement effect can be optimized are described. Experimental results show that the SNR of the readout image using this technique is superior to that obtained using high-pass filtering. The repeatability of this effect is confirmed by obtaining an edge enhancement result using two different Hughes LCLVs. The applicability of this effect to improve discrimination capability in optical pattern recognition is addressed. The results show that the Hughes LCLV can be used in both continuous tone and edge-enhancing modes by simply adjusting its bias conditions.

  15. Design and characterization of a real time particle radiography system based on scintillating optical fibers

    NASA Astrophysics Data System (ADS)

    Longhitano, F.; Lo Presti, D.; Bonanno, D. L.; Bongiovanni, D. G.; Leonora, E.; Randazzo, N.; Reito, S.; Sipala, V.; Gallo, G.

    2017-02-01

    The fabrication and characterization of a charged particle imaging system composed of a tracker and a residual range detector (RRD) is described. The tracker is composed of four layers of scintillating fibers (SciFi), 500 μm side square section, arranged to form two planes orthogonal to each other. The fibers are coupled to two Multi-Pixel Photon Counter (MPPC) arrays by means of a channel reduction system patented by the Istituto Nazionale di Fisica Nucleare (INFN) (Presti, 2015) [1]. Sixty parallel layers of the same fibers used in the tracker compose the RRD. The various layers are optically coupled to a MPPC array by means of wavelength shifting (WLS) fibers. The sensitive area of the two detectors is 9×9 cm2. The results of the measurements, acquired by the prototypes with CATANA (Cirrone, 2008) [2] proton beam, and a comparison with the simulations of the detectors are presented.

  16. Optical joint correlator for real-time image tracking and retinal surgery

    NASA Technical Reports Server (NTRS)

    Juday, Richard D. (Inventor)

    1991-01-01

    A method for tracking an object in a sequence of images is described. Such sequence of images may, for example, be a sequence of television frames. The object in the current frame is correlated with the object in the previous frame to obtain the relative location of the object in the two frames. An optical joint transform correlator apparatus is provided to carry out the process. Such joint transform correlator apparatus forms the basis for laser eye surgical apparatus where an image of the fundus of an eyeball is stabilized and forms the basis for the correlator apparatus to track the position of the eyeball caused by involuntary movement. With knowledge of the eyeball position, a surgical laser can be precisely pointed toward a position on the retina.

  17. Phase unwrapping algorithms for use in a true real-time optical body sensor system for use during radiotherapy.

    PubMed

    Parkhurst, James; Price, Gareth; Sharrock, Phil; Moore, Christopher

    2011-12-10

    An evaluation of the suitability of eight existing phase unwrapping algorithms to be used in a real-time optical body surface sensor based on Fourier fringe profilometry is presented. The algorithms are assessed on both the robustness of the results they give and their speed of execution. The algorithms are evaluated using four sets of real human body surface data, each containing five-hundred frames, obtained from patients undergoing radiotherapy, where fringe discontinuity is significant. We also present modifications to an existing algorithm, noncontinuous quality-guided path algorithm (NCQUAL), in order to decrease its execution time by a factor of 4 to make it suitable for use in a real-time system. The results obtained from the modified algorithm are compared with those of the existing algorithms. Three suitable algorithms were identified: two-stage noncontinuous quality-guided path algorithm (TSNCQUAL)-the modified algorithm presented here-for online processing and Flynn's minimum discontinuity algorithm (FLYNN) and preconditioned conjugate gradient method (PCG) algorithms for enhanced accuracy in off-line processing.

  18. Real-time monitoring of hemodynamic changes in tumor vessels during photoimmunotherapy using optical coherence tomography.

    PubMed

    Liang, Chia-Pin; Nakajima, Takahito; Watanabe, Rira; Sato, Kazuhide; Choyke, Peter L; Chen, Yu; Kobayashi, Hisataka

    2014-09-01

    Photoimmunotherapy (PIT) is a cell-specific cancer therapy based on an armed antibody conjugate that induces rapid and highly selective cancer cell necrosis after exposure to near-infrared (NIR) light. The PIT treatment also induces the superenhanced permeability and retention effect, which allows high concentrations of nanoparticles to accumulate in the tumor bed. In our pilot studies, optical coherence tomography (OCT) reveals dramatic hemodynamic changes during PIT. We developed and applied speckle variance analysis, Doppler flow measurement, bulk motion removal, and automatic region of interest selection to quantify vessel diameter and blood velocity within tumors in vivo. OCT imaging reveals that blood velocity in peripheral tumor vessels quickly drops below the detection limit while the vessel lumen remains open (4 vessels from 3 animals). On the other hand, control tumor vessels (receive NIR illumination but no PIT drug) do not show the sustained blood velocity drop (5 vessels from 3 animals). Ultraslow blood velocity could result in a long drug circulation time in tumor. Increase of the blood pool volume within the central tumor (shown in histology) may be the leading cause of the periphery blood velocity drop and could also increase the drug pool volume in tumor vessels.

  19. Real-time monitoring of hemodynamic changes in tumor vessels during photoimmunotherapy using optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Liang, Chia-Pin; Nakajima, Takahito; Watanabe, Rira; Sato, Kazuhide; Choyke, Peter L.; Chen, Yu; Kobayashi, Hisataka

    2014-09-01

    Photoimmunotherapy (PIT) is a cell-specific cancer therapy based on an armed antibody conjugate that induces rapid and highly selective cancer cell necrosis after exposure to near-infrared (NIR) light. The PIT treatment also induces the superenhanced permeability and retention effect, which allows high concentrations of nanoparticles to accumulate in the tumor bed. In our pilot studies, optical coherence tomography (OCT) reveals dramatic hemodynamic changes during PIT. We developed and applied speckle variance analysis, Doppler flow measurement, bulk motion removal, and automatic region of interest selection to quantify vessel diameter and blood velocity within tumors in vivo. OCT imaging reveals that blood velocity in peripheral tumor vessels quickly drops below the detection limit while the vessel lumen remains open (4 vessels from 3 animals). On the other hand, control tumor vessels (receive NIR illumination but no PIT drug) do not show the sustained blood velocity drop (5 vessels from 3 animals). Ultraslow blood velocity could result in a long drug circulation time in tumor. Increase of the blood pool volume within the central tumor (shown in histology) may be the leading cause of the periphery blood velocity drop and could also increase the drug pool volume in tumor vessels.

  20. Real Time Gabor-Domain Optical Coherence Microscopy for 3D Imaging.

    PubMed

    Rolland, Jannick P; Canavesi, Cristina; Tankam, Patrice; Cogliati, Andrea; Lanis, Mara; Santhanam, Anand P

    2016-01-01

    Fast, robust, nondestructive 3D imaging is needed for the characterization of microscopic tissue structures across various clinical applications. A custom microelectromechanical system (MEMS)-based 2D scanner was developed to achieve, together with a multi-level GPU architecture, 55 kHz fast-axis A-scan acquisition in a Gabor-domain optical coherence microscopy (GD-OCM) custom instrument. GD-OCM yields high-definition micrometer-class volumetric images. A dynamic depth of focusing capability through a bio-inspired liquid lens-based microscope design, as in whales' eyes, was developed to enable the high definition instrument throughout a large field of view of 1 mm3 volume of imaging. Developing this technology is prime to enable integration within the workflow of clinical environments. Imaging at an invariant resolution of 2 μm has been achieved throughout a volume of 1 × 1 × 0.6 mm3, acquired in less than 2 minutes. Volumetric scans of human skin in vivo and an excised human cornea are presented.

  1. Mitochondrial function and tissue vitality: bench-to-bedside real-time optical monitoring system

    NASA Astrophysics Data System (ADS)

    Mayevsky, Avraham; Walden, Raphael; Pewzner, Eliyahu; Deutsch, Assaf; Heldenberg, Eitan; Lavee, Jacob; Tager, Salis; Kachel, Erez; Raanani, Ehud; Preisman, Sergey; Glauber, Violete; Segal, Eran

    2011-06-01

    Background: The involvement of mitochondria in pathological states, such as neurodegenerative diseases, sepsis, stroke, and cancer, are well documented. Monitoring of nicotinamide adenine dinucleotide (NADH) fluorescence in vivo as an intracellular oxygen indicator was established in 1950 to 1970 by Britton Chance and collaborators. We use a multiparametric monitoring system enabling assessment of tissue vitality. In order to use this technology in clinical practice, the commercial developed device, the CritiView (CRV), is tested in animal models as well as in patients. Methods and Results: The new CRV enables the optical monitoring of four different parameters, representing the energy balance of various tissues in vivo. Mitochondrial NADH is measured by surface fluorometry/reflectometry. In addition, tissue microcirculatory blood flow, tissue reflectance and oxygenation are measured as well. The device is tested both in vitro and in vivo in a small animal model and in preliminary clinical trials in patients undergoing vascular or open heart surgery. In patients, the monitoring is started immediately after the insertion of a three-way Foley catheter (urine collection) to the patient and is stopped when the patient is discharged from the operating room. The results show that monitoring the urethral wall vitality provides information in correlation to the surgical procedure performed.

  2. Nondestructive inspection of tagged composites using real-time magneto-optic imaging

    NASA Astrophysics Data System (ADS)

    Fitzpatrick, Gerald L.; Thome, David K.; Skaugset, Richard L.; Shih, William C.

    1996-11-01

    While magneto-optic/eddy current imagers (MOIs) are widely used for the nondestructive inspection (NDI) of aging aluminum airframes, they are not currently used to inspect aerospace composites for two reasons. First, most composites are essentially electrical insulators, which makes it difficult for the MOI to induce sufficient eddy currents in these materials for imaging. Second, unlike steel, which can be easily magnetized and inspected using the MOI, most composites are not appreciably magnetic. Exceptions to these two stated limitations are composites containing electrically conducting 'screens' of aluminum or copper for lighting-protection, 'stealthy' military composites containing iron particles for radar absorption, and some commercial composites containing nickel-coated carbon fibers for lighting protection and/or electromagnetic shielding. These existing composites can be inspected, to one degree or another,with the MOI. Moreover, by 'tagging' the matrix component of newly-manufactured composites with magnetic oxides, excellent MOI images of cracks, voids, potential disbonds and defects in fiber-weave are possible. Experimental results presented here demonstrate these facts. It is anticipated that when this new technology is perfected, and when magnetic 'tagging' at the time of manufacture becomes an accepted practice, the MOI will be widely used for the NDI of composites during their manufacture, in-service inspection or repair.

  3. Ratiometric optical PEBBLE nanosensors for real-time magnesium ion concentrations inside viable cells.

    PubMed

    Park, Edwin J; Brasuel, Murphy; Behrend, Caleb; Philbert, Martin A; Kopelman, Raoul

    2003-08-01

    This paper presents the development and characterization of a highly selective magnesium fluorescent optical nanosensor, made possible by PEBBLE (probe encapsulated by biologically localized embedding) technology. A ratiometric sensor has been developed by co-immobilizing a dye that is sensitive to and highly selective for magnesium, with a reference dye in a matrix. The sensors are prepared via a microemulsion polymerization process, which entraps the sensing components inside a polymer matrix. The resultant spherical sensors are approximately 40 nm in diameter. The Coumarin 343 (C343) dye, which by itself does not enter the cell, when immobilized in a PEBBLE is used as the magnesium-selective agent that provides the high and necessary selectivity over other intracellular ions, such as Ca2+, Na+, and K+. The dynamic range of these sensors was 1-30 mM, with a linear range from 1 to 10 mM, with a response time of <4 s. In contrast to free dye, these nano-optodes are not perturbed by proteins. They are fully reversible and exhibit minimal leaching and photobleaching over extended periods of time. In vitro intracellular changes in Mg2+ concentration were monitored in C6 glioma cells, which remained viable after PEBBLE delivery via gene gun injection. The selectivity for Mg2+ along with the biocompatibility of the matrix provides a new and reliable tool for intracellular magnesium measurements.

  4. Real-Time Dynamic Atomic Spectroscopy Using Electro-Optic Frequency Combs

    NASA Astrophysics Data System (ADS)

    Hébert, Nicolas Bourbeau; Michaud-Belleau, Vincent; Perrella, Christopher; Truong, Gar-Wing; Anstie, James D.; Stace, Thomas M.; Genest, Jérôme; Luiten, Andre N.

    2016-10-01

    Spectroscopy is a key technology for both fundamental and applied science. A long-held desire has been the development of a means to continuously acquire broadband spectral data with simultaneous high time and frequency resolution. Frequency-comb technology can open this door: here, we use a spectroscopic technique based on an electro-optic comb to make continuous observations of cesium vapor across a 3.2-GHz spectral bandwidth with a 2-μ s time resolution and with 10-MHz frequency sampling. We use a rapidly switched pump laser to burn narrow features into the spectral line and study the response to this step perturbation. This examination allows us to see a number of unexpected effects, including the temporal evolution of the bandwidth, the amplitude, and the frequency of these burnt features. We also report on the previously unobserved effect of radiation reabsorption, which slowly produces a broad pedestal of perturbation around each feature. We present models that can explain these dynamical effects.

  5. Real-time optical OFDM transmissions with spectral efficiency up to 6.93 bit/s/Hz over 50 km SSMF IMDD systems

    NASA Astrophysics Data System (ADS)

    Zhang, Qianwu; Liu, Min; Chen, Jian; Dun, Han; Zhang, Junjie; Li, Yingchun; Song, Yingxiong; Wang, Min

    2017-03-01

    Real-time optical OFDM transmissions based on FPGA with 6.93 bit/s/Hz spectral efficiency by using simple directly modulated DFB laser and DAC/ADC with sampling rate as low as 2GS/s without inline amplifier are experimentally demonstrated. 7.09-Gb/s OFDM signals with subcarrier modulation format up to 512-QAM can be successfully transmitted over 50-km SSMF with adopted HD-FEC (3.8×10-3). The robustness of system performance is also extensively explored to determine the tolerance to DAC/ADC resolution mismatch. It is shown that aggregate system capacity can be maintained almost constant for DAC/ADC resolution mismatch as large as 3bits due to the utilization of adaptive bit and power loading scheme. On the other hand, for a certain system configuration and an expected signal line rate, it also gives a reference minimum DAC/ADC boundary for practical deployment.

  6. Results from GROCSE, A Real-Time Search for the Optical Counterparts of Gamma-Ray Bursts

    NASA Astrophysics Data System (ADS)

    Akerlof, Carl; Lee, Brian; Barthelmy, Scott; Cline, Thomas; Gehrels, Neil; Ables, Elden; Bionta, Richard; Ott, Linda; Park, Hye-Sook; Fishman, Gerald; Kouveliotou, Chryssa; Meegan, Charles; Ferguson, Donald

    1994-12-01

    Since January 12, 1994, an experiment called GROCSE (Gamma-Ray Optical Counterpart Search Experiment) has been monitoring the night sky for the optical counterparts of gamma-ray bursts. The basic detector consists of an 8.9 cm aperture electronic camera attached to a rapid slewing computer-controlled mount. This device is activated by the real-time telemetry data stream from the BATSE instrument onboard the Compton Gamma-Ray Observatory. The BATSE signals are filtered and broadcast via the BACODINE network to Lawrence Livermore National Laboratory using the UNIX socket communication protocol linked via Internet. The typical response time to obtain the first image is approximately 15 seconds following the initial burst detection. The field of view of the camera is restricted to 0.18 sterdians to match the online angular position errors associated with the BACODINE GRB coordinate estimates. Under dark skys, the limiting detection magnitude is 8.5. By October 1994, the GROCSE camera has been triggered by seven BATSE bursts. Data from these events are being analyzed to provide either a detection or an upper limit for GRB optical luminosity. Results will be presented for the ratio of optical to gamma-ray intensity. A second generation camera system is currently under development that is expected to push the limiting magnitude to approximately m_v = 13. The status of this effort will be briefly reported.

  7. A plasmonic optical fiber patterned by template transfer as a high-performance flexible nanoprobe for real-time biosensing.

    PubMed

    Jia, Peipei; Yang, Jun

    2014-08-07

    Surface plasmon resonance (SPR) on metal nanostructures offers a promising route for manipulation and interrogation of light in the subwavelength regime. However, the utility of SPR structures is largely limited by currently used complex nanofabrication methods and relatively sophisticated optical components. Here to relieve these restrictions, plasmonic optical fibers are constructed by transferring periodic metal nanostructures from patterned templates onto endfaces of optical fibers using an epoxy adhesive. Patterned metal structures are generally extended from two-dimensional (2D) nanohole arrays to one-dimensional (1D) nanoslit arrays. By controlling the viscosity of the adhesive layer, diverse surface topographies of metal structures are realized with the same template. We design a special plasmonic fiber that simultaneously implements multimode refractive index sensing (transmission and reflection) with remarkably narrow linewidth (6.6 nm) and high figure of merit (60.7), which are both among the best reported values for SPR sensors. We further demonstrate a real-time immunoassay relying on our plasmonic fiber integrated with a special flow cell. Plasmonic optical fibers also take advantages of excellent stability during fiber bending and capability of spectrum filtering. These features enable our plasmonic fibers to open up an alternative avenue for the general community in biosensing and nanoplasmonics.

  8. Real-time optical detection of single human and bacterial viruses based on dark-field interferometry.

    PubMed

    Mitra, Anirban; Ignatovich, Filipp; Novotny, Lukas

    2012-01-15

    The rapid and sensitive detection and characterization of human viruses and bacteriophage is extremely important in a variety of fields, such as medical diagnostics, immunology and vaccine research, and environmental contamination and quality control. We introduce an optical detection scheme for real-time and label-free detection of human viruses and bacteriophage as small as ~24 nm in radius. Combining the advantages of heterodyne interferometry and dark-field microscopy, this label-free method enables us to detect and characterize various biological nanoparticles with unsurpassed sensitivity and selectivity. We demonstrate the high sensitivity and precision of the method by analyzing a mixture containing HIV virus and bacteriophage. The method also resolves the distribution of small nano-impurities (~20-30 nm) in clinically relevant virus samples.

  9. Real-time approach to the optical properties of solids and nanostructures: Time-dependent Bethe-Salpeter equation

    NASA Astrophysics Data System (ADS)

    Attaccalite, C.; Grüning, M.; Marini, A.

    2011-12-01

    Many-body effects are known to play a crucial role in the electronic and optical properties of solids and nanostructures. Nevertheless, the majority of theoretical and numerical approaches able to capture the influence of Coulomb correlations are restricted to the linear response regime. In this work, we introduce an approach based on a real-time solution of the electronic dynamics. The proposed approach reduces to the well-known Bethe-Salpeter equation in the linear limit regime and it makes it possible, at the same time, to investigate correlation effects in nonlinear phenomena. We show the flexibility and numerical stability of the proposed approach by calculating the dielectric constants and the effect of a strong pulse excitation in bulk h-BN.

  10. Real-time monitoring of vibration fringe patterns by optical reconstruction of digital holograms: mode beating detection.

    PubMed

    Demoli, Nazif

    2006-03-20

    A new technique capable of monitoring two-dimensional vibration fringe patterns in real-time is presented. It is based on optical reconstructing of time-averaged digital holograms. Recording of the holograms is realized in a quasi-Fourier off-axis setup whilst reconstructing in an extended Fourier transform setup capable of spatial filtering of the hologram reconstructions. The effectiveness of the proposed device, tested on a silicon wafer, was verified by performing operations such as monitoring of vibration modes, adjusting the hologram recording parameters, or searching for resonant frequencies. Additionally, mode beating between two distant vibration frequencies is observed and reported. The proposed technique is suitable for various scientific and industrial applications.

  11. Note: Real time optical sensing of alpha-radiation emitting radioactive aerosols based on solid state nuclear track detector

    NASA Astrophysics Data System (ADS)

    Kulkarni, A.; Ha, S.; Joshirao, P.; Manchanda, V.; Bak, M. S.; Kim, T.

    2015-06-01

    A sensitive radioactive aerosols sensor has been designed and developed. Its design guidance is based on the need for a low operational cost and reliable measurements to provide daily aerosol monitoring. The exposure of diethylene-glycol bis (allylcarbonate) to radiation causes modification of its physico-chemical properties like surface roughness and reflectance. In the present study, optical sensor based on the reflectance measurement has been developed with an aim to monitor real time presence of alpha radioactive aerosols emitted from thorium nitrate hydrate. The results shows that the fabricated sensor can detect 0.0157 kBq to 0.1572 kBq of radio activity by radioactive aerosols generated from (Th(NO3)4 ṡ 5H2O) at 0.1 ml/min flow rate. The proposed instrument will be helpful to monitor radioactive aerosols in/around a nuclear facility, building construction sites, mines, and granite polishing factories.

  12. Note: Real time optical sensing of alpha-radiation emitting radioactive aerosols based on solid state nuclear track detector.

    PubMed

    Kulkarni, A; Ha, S; Joshirao, P; Manchanda, V; Bak, M S; Kim, T

    2015-06-01

    A sensitive radioactive aerosols sensor has been designed and developed. Its design guidance is based on the need for a low operational cost and reliable measurements to provide daily aerosol monitoring. The exposure of diethylene-glycol bis (allylcarbonate) to radiation causes modification of its physico-chemical properties like surface roughness and reflectance. In the present study, optical sensor based on the reflectance measurement has been developed with an aim to monitor real time presence of alpha radioactive aerosols emitted from thorium nitrate hydrate. The results shows that the fabricated sensor can detect 0.0157 kBq to 0.1572 kBq of radio activity by radioactive aerosols generated from (Th(NO3)4 ⋅ 5H2O) at 0.1 ml/min flow rate. The proposed instrument will be helpful to monitor radioactive aerosols in/around a nuclear facility, building construction sites, mines, and granite polishing factories.

  13. Note: Real time optical sensing of alpha-radiation emitting radioactive aerosols based on solid state nuclear track detector

    SciTech Connect

    Kulkarni, A.; Bak, M. S. E-mail: moonsoo@skku.edu; Ha, S.; Joshirao, P.; Manchanda, V.; Kim, T. E-mail: moonsoo@skku.edu

    2015-06-15

    A sensitive radioactive aerosols sensor has been designed and developed. Its design guidance is based on the need for a low operational cost and reliable measurements to provide daily aerosol monitoring. The exposure of diethylene-glycol bis (allylcarbonate) to radiation causes modification of its physico-chemical properties like surface roughness and reflectance. In the present study, optical sensor based on the reflectance measurement has been developed with an aim to monitor real time presence of alpha radioactive aerosols emitted from thorium nitrate hydrate. The results shows that the fabricated sensor can detect 0.0157 kBq to 0.1572 kBq of radio activity by radioactive aerosols generated from (Th(NO{sub 3}){sub 4} ⋅ 5H{sub 2}O) at 0.1 ml/min flow rate. The proposed instrument will be helpful to monitor radioactive aerosols in/around a nuclear facility, building construction sites, mines, and granite polishing factories.

  14. Graphics processing unit accelerated intensity-based optical coherence tomography angiography using differential frames with real-time motion correction.

    PubMed

    Watanabe, Yuuki; Takahashi, Yuhei; Numazawa, Hiroshi

    2014-02-01

    We demonstrate intensity-based optical coherence tomography (OCT) angiography using the squared difference of two sequential frames with bulk-tissue-motion (BTM) correction. This motion correction was performed by minimization of the sum of the pixel values using axial- and lateral-pixel-shifted structural OCT images. We extract the BTM-corrected image from a total of 25 calculated OCT angiographic images. Image processing was accelerated by a graphics processing unit (GPU) with many stream processors to optimize the parallel processing procedure. The GPU processing rate was faster than that of a line scan camera (46.9 kHz). Our OCT system provides the means of displaying structural OCT images and BTM-corrected OCT angiographic images in real time.

  15. Telescope Adaptive Optics Code

    SciTech Connect

    Phillion, D.

    2005-07-28

    The Telescope AO Code has general adaptive optics capabilities plus specialized models for three telescopes with either adaptive optics or active optics systems. It has the capability to generate either single-layer or distributed Kolmogorov turbulence phase screens using the FFT. Missing low order spatial frequencies are added using the Karhunen-Loeve expansion. The phase structure curve is extremely dose to the theoreUcal. Secondly, it has the capability to simulate an adaptive optics control systems. The default parameters are those of the Keck II adaptive optics system. Thirdly, it has a general wave optics capability to model the science camera halo due to scintillation from atmospheric turbulence and the telescope optics. Although this capability was implemented for the Gemini telescopes, the only default parameter specific to the Gemini telescopes is the primary mirror diameter. Finally, it has a model for the LSST active optics alignment strategy. This last model is highly specific to the LSST

  16. [Adaptive optics for ophthalmology].

    PubMed

    Saleh, M

    2016-04-01

    Adaptive optics is a technology enhancing the visual performance of an optical system by correcting its optical aberrations. Adaptive optics have already enabled several breakthroughs in the field of visual sciences, such as improvement of visual acuity in normal and diseased eyes beyond physiologic limits, and the correction of presbyopia. Adaptive optics technology also provides high-resolution, in vivo imaging of the retina that may eventually help to detect the onset of retinal conditions at an early stage and provide better assessment of treatment efficacy.

  17. Efficient Hardware Implementation of the Horn-Schunck Algorithm for High-Resolution Real-Time Dense Optical Flow Sensor

    PubMed Central

    Komorkiewicz, Mateusz; Kryjak, Tomasz; Gorgon, Marek

    2014-01-01

    This article presents an efficient hardware implementation of the Horn-Schunck algorithm that can be used in an embedded optical flow sensor. An architecture is proposed, that realises the iterative Horn-Schunck algorithm in a pipelined manner. This modification allows to achieve data throughput of 175 MPixels/s and makes processing of Full HD video stream (1, 920 × 1, 080 @ 60 fps) possible. The structure of the optical flow module as well as pre- and post-filtering blocks and a flow reliability computation unit is described in details. Three versions of optical flow modules, with different numerical precision, working frequency and obtained results accuracy are proposed. The errors caused by switching from floating- to fixed-point computations are also evaluated. The described architecture was tested on popular sequences from an optical flow dataset of the Middlebury University. It achieves state-of-the-art results among hardware implementations of single scale methods. The designed fixed-point architecture achieves performance of 418 GOPS with power efficiency of 34 GOPS/W. The proposed floating-point module achieves 103 GFLOPS, with power efficiency of 24 GFLOPS/W. Moreover, a 100 times speedup compared to a modern CPU with SIMD support is reported. A complete, working vision system realized on Xilinx VC707 evaluation board is also presented. It is able to compute optical flow for Full HD video stream received from an HDMI camera in real-time. The obtained results prove that FPGA devices are an ideal platform for embedded vision systems. PMID:24526303

  18. Experimental demonstration of a real-time high-throughput digital DC blocker for compensating ADC imperfections in optical fast-OFDM receivers.

    PubMed

    Zhang, Lu; Ouyang, Xing; Shao, Xiaopeng; Zhao, Jian

    2016-06-27

    Performance degradation induced by the DC components at the output of real-time analogue-to-digital converter (ADC) is experimentally investigated for optical fast-OFDM receiver. To compensate this degradation, register transfer level (RTL) circuits for real-time digital DC blocker with 20GS/s throughput are proposed and implemented in field programmable gate array (FPGA). The performance of the proposed real-time digital DC blocker is experimentally investigated in a 15Gb/s optical fast-OFDM system with intensity modulation and direct detection over 40 km standard single-mode fibre. The results show that the fixed-point DC blocker has negligible performance penalty compared to the offline floating point one, and can overcome the error floor of the fast OFDM receiver caused by the DC components from the real-time ADC output.

  19. An improved optical flow tracking technique for real-time MR-guided beam therapies in moving organs.

    PubMed

    Zachiu, C; Papadakis, N; Ries, M; Moonen, C; Denis de Senneville, B

    2015-12-07

    Magnetic resonance (MR) guided high intensity focused ultrasound and external beam radiotherapy interventions, which we shall refer to as beam therapies/interventions, are promising techniques for the non-invasive ablation of tumours in abdominal organs. However, therapeutic energy delivery in these areas becomes challenging due to the continuous displacement of the organs with respiration. Previous studies have addressed this problem by coupling high-framerate MR-imaging with a tracking technique based on the algorithm proposed by Horn and Schunck (H and S), which was chosen due to its fast convergence rate and highly parallelisable numerical scheme. Such characteristics were shown to be indispensable for the real-time guidance of beam therapies. In its original form, however, the algorithm is sensitive to local grey-level intensity variations not attributed to motion such as those that occur, for example, in the proximity of pulsating arteries.In this study, an improved motion estimation strategy which reduces the impact of such effects is proposed. Displacements are estimated through the minimisation of a variation of the H and S functional for which the quadratic data fidelity term was replaced with a term based on the linear L(1)norm, resulting in what we have called an L(2)-L(1) functional.The proposed method was tested in the livers and kidneys of two healthy volunteers under free-breathing conditions, on a data set comprising 3000 images equally divided between the volunteers. The results show that, compared to the existing approaches, our method demonstrates a greater robustness to local grey-level intensity variations introduced by arterial pulsations. Additionally, the computational time required by our implementation make it compatible with the work-flow of real-time MR-guided beam interventions.To the best of our knowledge this study was the first to analyse the behaviour of an L(1)-based optical flow functional in an applicative context: real-time MR

  20. Reliability aspects of the future hybrid optical network and quality of service issues for real time and packet traffic

    NASA Astrophysics Data System (ADS)

    Marciniak, Marian

    2004-09-01

    While Internet traffic increases dramatically and now it exceeds the almost stable voice traffic, new efficient solutions for network architecture are sought. One of the main problems to be resolved is the basic concept of the future network performance. While actual networks are based on 'circuit switching' connection networks with IP packets forwarded given the network offers some free resources, a number of researchers foresee the future network as a 'packet switched' connectionless network which would support also the traditional real time services with phone calls as the most representative example. This is driven by the growing use of voice over IP services. Unfortunately, the Quality of Service is actually not sufficient for wide acceptance of that technique. In fact, Internet is based on the 'best-effort' principle in what it fudamentally differs from circuit-switched network which has been developed uniquely to provide excellent voice quality during last century. Moreover, the reliability of the network is a crucial factor in emergency communications, what has recently became a subject of intense activity at national and international scale. As a consequence, the idea of dropping the converged network with voice-over-IP concept is hard to accept for a great majority and those expressing it are usually looked as strange or even dangerous individuals. Nevertheless, those fundamental difficulties become obvious for a growing number of people who approach the future network evolution more fundamentally, and try to trace reasonable and economic ways of the future network development. The author of this paper has extensively developed a concept of a "hybrid network" which consists of real-time subnetwork and packet network separated in the optical frequency domain that will be discussed in detail in this paper. This optimized network concept is intended to guarantee both reliability of the network and Quality of Service as well.

  1. In Site Analysis of a High Temperature Cure Reaction in Real Time Using Modulated Fiber-Optic FT-Raman Spectroscopy

    NASA Technical Reports Server (NTRS)

    Cooper, John; Aust, Jeffrey F.; Wise, Kent L.; Jensen, Brian J.

    1999-01-01

    The vibrational spectrum of a high temperature (330 C) polymerization reaction was successfully monitored in real time using a modulated fiber-optic FT-Raman spectrometer. A phenylethynyl terminated monomer was cured, and spectral evidence for two different reaction products was acquired. The products are a conjugated polyene chain and a cyclized trimer. This is the first report describing the use of FT-Raman spectroscopy to monitor a high temperature (greater than 250 C) reaction in real time.

  2. Driver Code for Adaptive Optics

    NASA Technical Reports Server (NTRS)

    Rao, Shanti

    2007-01-01

    A special-purpose computer code for a deformable-mirror adaptive-optics control system transmits pixel-registered control from (1) a personal computer running software that generates the control data to (2) a circuit board with 128 digital-to-analog converters (DACs) that generate voltages to drive the deformable-mirror actuators. This program reads control-voltage codes from a text file, then sends them, via the computer s parallel port, to a circuit board with four AD5535 (or equivalent) chips. Whereas a similar prior computer program was capable of transmitting data to only one chip at a time, this program can send data to four chips simultaneously. This program is in the form of C-language code that can be compiled and linked into an adaptive-optics software system. The program as supplied includes source code for integration into the adaptive-optics software, documentation, and a component that provides a demonstration of loading DAC codes from a text file. On a standard Windows desktop computer, the software can update 128 channels in 10 ms. On Real-Time Linux with a digital I/O card, the software can update 1024 channels (8 boards in parallel) every 8 ms.

  3. Volmer-Weber growth stages of polycrystalline metal films probed by in situ and real-time optical diagnostics

    NASA Astrophysics Data System (ADS)

    Abadias, G.; Simonot, L.; Colin, J. J.; Michel, A.; Camelio, S.; Babonneau, D.

    2015-11-01

    The Volmer-Weber growth of high-mobility metal films is associated with the development of a complex compressive-tensile-compressive stress behavior as the film deposition proceeds through nucleation of islands, coalescence, and formation of a continuous layer. The tensile force maximum has been attributed to the end of the islands coalescence stage, based on ex situ morphological observations. However, microstructural rearrangements are likely to occur in such films during post-deposition, somewhat biasing interpretations solely based on ex situ analysis. Here, by combining two simultaneous in situ and real-time optical sensing techniques, based on surface differential reflectance spectroscopy (SDRS) and change in wafer curvature probed by multibeam optical stress sensor (MOSS), we provide direct evidence that film continuity does coincide with tensile stress maximum during sputter deposition of a series of metal (Ag, Au, and Pd) films on amorphous SiOx. Stress relaxation after growth interruption was testified from MOSS, whose magnitude scaled with adatom mobility, while no change in SDRS signal could be revealed, ruling out possible changes of the surface roughness at the micron scale.

  4. The aquatic real-time monitoring network; in-situ optical sensors for monitoring the nation's water quality

    USGS Publications Warehouse

    Pellerin, Brian A.; Bergamaschi, Brian A.; Murdoch, Peter S.; Downing, Bryan D.; Saraceno, John Franco; Aiken, George R.; Striegl, Robert G.

    2011-01-01

    Floods, hurricanes, and longer-term changes in climate and land use can have profound effects on water quality due to shifts in hydrologic flow paths, water residence time, precipitation patterns, connectivity between rivers and uplands, and many other factors. In order to understand and respond to changes in hydrology and water quality, resource managers and policy makers have a need for accurate and early indicators, as well as the ability to assess possible mechanisms and likely outcomes. In-situ optical sensors-those making continuous measurements of constituents by absorbance or fluorescence properties in the environment at timescales of minutes to years-have a long history in oceanography for developing highly resolved concentrations and fluxes, but are not commonly used in freshwater systems. The United States Geological Survey (USGS) has developed the Aquatic Real-Time Monitoring Network, with high-resolution optical data collection for organic carbon, nutrients, and sediment in large coastal rivers, along with continuous measurements of discharge, water temperature, and dissolved inorganic carbon. The collecting of continuous water-quality data in the Nation?s waterways has revealed temporal trends and spatial patterns in constituents that traditional sampling approaches fail to capture, and will serve a critical role in monitoring, assessment and decision-making in a rapidly changing landscape.

  5. Real-Time Display Of 3-D Computed Holograms By Scanning The Image Of An Acousto-Optic Modulator

    NASA Astrophysics Data System (ADS)

    Kollin, Joel S.; Benton, Stephen A.; Jepsen, Mary Lou

    1989-10-01

    The invention of holography has sparked hopes for a three-dimensional electronic imaging systems analogous to television. Unfortunately, the extraordinary spatial detail of ordinary holographic recordings requires unattainable bandwidth and display resolution for three-dimensional moving imagery, effectively preventing their commercial development. However, the essential bandwidth of holographic images can be reduced enough to permit their transmission through fiber optic or coaxial cable, and the required resolution or space-bandwidth product of the display can be obtained by raster scanning the image of a commercially available acousto-optic modulator. No film recording or other photographic intermediate step is necessary as the projected modulator image is viewed directly. The design and construction of a working demonstration of the principles involved is also presented along with a discussion of engineering considerations in the system design. Finally, the theoretical and practical limitations of the system are addressed in the context of extending the system to real-time transmission of moving holograms synthesized from views of real and computer-generated three-dimensional scenes.

  6. Generation of optical OFDM signals using 21.4 GS/s real time digital signal processing.

    PubMed

    Benlachtar, Yannis; Watts, Philip M; Bouziane, Rachid; Milder, Peter; Rangaraj, Deepak; Cartolano, Anthony; Koutsoyannis, Robert; Hoe, James C; Püschel, Markus; Glick, Madeleine; Killey, Robert I

    2009-09-28

    We demonstrate a field programmable gate array (FPGA) based optical orthogonal frequency division multiplexing (OFDM) transmitter implementing real time digital signal processing at a sample rate of 21.4 GS/s. The QPSK-OFDM signal is generated using an 8 bit, 128 point inverse fast Fourier transform (IFFT) core, performing one transform per clock cycle at a clock speed of 167.2 MHz and can be deployed with either a direct-detection or a coherent receiver. The hardware design and the main digital signal processing functions are described, and we show that the main performance limitation is due to the low (4-bit) resolution of the digital-to-analog converter (DAC) and the 8-bit resolution of the IFFT core used. We analyze the back-to-back performance of the transmitter generating an 8.36 Gb/s optical single sideband (SSB) OFDM signal using digital up-conversion, suitable for direct-detection. Additionally, we use the device to transmit 8.36 Gb/s SSB OFDM signals over 200 km of uncompensated standard single mode fiber achieving an overall BER<10(-3).

  7. Retinal Imaging: Adaptive Optics

    NASA Astrophysics Data System (ADS)

    Goncharov, A. S.; Iroshnikov, N. G.; Larichev, Andrey V.

    This chapter describes several factors influencing the performance of ophthalmic diagnostic systems with adaptive optics compensation of human eye aberration. Particular attention is paid to speckle modulation, temporal behavior of aberrations, and anisoplanatic effects. The implementation of a fundus camera with adaptive optics is considered.

  8. Adaptive optics ophthalmoscopy.

    PubMed

    Roorda, A

    2000-01-01

    Retinal images in the human eye are normally degraded because we are forced to use the optical system of the human eye--which is fraught with aberrations--as the objective lens. The recent application of adaptive optics technology to measure and compensate for these aberrations has produced retinal images in human eyes with unprecedented resolution. The adaptive optics ophthalmoscope is used to take pictures of photoreceptors and capillaries and to study spectral and angular tuning properties of individual photoreceptors. Application of adaptive optics technology for ophthalmoscopy promises continued progress toward understanding the basic properties of the living human retina and also for clinical applications.

  9. Adaptive error detection for HDR/PDR brachytherapy: Guidance for decision making during real-time in vivo point dosimetry

    SciTech Connect

    Kertzscher, Gustavo Andersen, Claus E.; Tanderup, Kari

    2014-05-15

    Purpose: This study presents an adaptive error detection algorithm (AEDA) for real-timein vivo point dosimetry during high dose rate (HDR) or pulsed dose rate (PDR) brachytherapy (BT) where the error identification, in contrast to existing approaches, does not depend on an a priori reconstruction of the dosimeter position. Instead, the treatment is judged based on dose rate comparisons between measurements and calculations of the most viable dosimeter position provided by the AEDA in a data driven approach. As a result, the AEDA compensates for false error cases related to systematic effects of the dosimeter position reconstruction. Given its nearly exclusive dependence on stable dosimeter positioning, the AEDA allows for a substantially simplified and time efficient real-time in vivo BT dosimetry implementation. Methods: In the event of a measured potential treatment error, the AEDA proposes the most viable dosimeter position out of alternatives to the original reconstruction by means of a data driven matching procedure between dose rate distributions. If measured dose rates do not differ significantly from the most viable alternative, the initial error indication may be attributed to a mispositioned or misreconstructed dosimeter (false error). However, if the error declaration persists, no viable dosimeter position can be found to explain the error, hence the discrepancy is more likely to originate from a misplaced or misreconstructed source applicator or from erroneously connected source guide tubes (true error). Results: The AEDA applied on twoin vivo dosimetry implementations for pulsed dose rate BT demonstrated that the AEDA correctly described effects responsible for initial error indications. The AEDA was able to correctly identify the major part of all permutations of simulated guide tube swap errors and simulated shifts of individual needles from the original reconstruction. Unidentified errors corresponded to scenarios where the dosimeter position was

  10. WE-G-BRD-03: Development of a Real-Time Optical Tracking Goggle System (OTGS) for Intracranial Stereotactic Radiotherapy

    SciTech Connect

    Mittauer, K; Yan, G; Lu, B; Barraclough, B; Li, J; Liu, C

    2014-06-15

    Purpose: Optical tracking systems (OTS) are an acceptable alternative to frame-based stereotactic radiotherapy (SRT). However, current surface-based OTS lack the ability to target exclusively rigid/bony anatomical features. We propose a novel marker-based optical tracking goggle system (OTGS) that provides real-time guidance based on the nose/facial bony anatomy. This ongoing study involves the development and characterization of the OTGS for clinical implementation in intracranial stereotactic radiotherapy. Methods: The OTGS consists of eye goggles, a custom thermoplastic nosepiece, and 6 infrared markers pre-attached to the goggles. A phantom and four healthy volunteers were used to evaluate the calibration/registration accuracy, intrafraction accuracy, interfraction reproducibility, and end-to-end accuracy of the OTGS. The performance of the OTGS was compared with that of the frameless SonArray system and cone-beam computed tomography (CBCT) for volunteer and phantom cases, respectively. The performance of the OTGS with commercial immobilization devices and under treatment conditions (i.e., couch rotation and translation range) was also evaluated. Results: The difference in the calibration/registration accuracy of 24 translations or rotation combinations between CBCT and in-house OTS software was within 0.5 mm/0.4°. The mean intrafraction and interfraction accuracy among the volunteers was 0.004+/−0.4mm with −0.09+/−0.5° (n=6,170) and −0.26+/−0.8mm with 0.15+/0.8° (n=11), respectively. The difference in end-to-end accuracy between the OTGS and CBCT was within 1.3 mm/1.1°. The predetermined marker pattern (1) minimized marker occlusions, (2) allowed for continuous tracking for couch angles +/− 90°, (3) and eliminated individual marker misplacement. The device was feasible with open and half masks for immobilization. Conclusion: Bony anatomical localization eliminated potential errors due to facial hair changes and/or soft tissue deformation. The

  11. Real-time detection of DNA hybridization and melting on oligonucleotide arrays by using optical wave guides.

    PubMed Central

    Stimpson, D I; Hoijer, J V; Hsieh, W T; Jou, C; Gordon, J; Theriault, T; Gamble, R; Baldeschwieler, J D

    1995-01-01

    The challenge of the Human Genome Project is to increase the rate of DNA sequence acquisition by two orders of magnitude to complete sequencing of the human genome by the year 2000. The present work describes a rapid detection method using a two-dimensional optical wave guide that allows measurement of real-time binding or melting of a light-scattering label on a DNA array. A particulate label on the target DNA acts as a light-scattering source when illuminated by the evanescent wave of the wave guide and only the label bound to the surface generates a signal. Imaging/visual examination of the scattered light permits interrogation of the entire array simultaneously. Hybridization specificity is equivalent to that obtained with a conventional system using autoradiography. Wave guide melting curves are consistent with those obtained in the liquid phase and single-base discrimination is facile. Dilution experiments showed an apparent lower limit of detection at 0.4 nM oligonucleotide. This performance is comparable to the best currently known fluorescence-based systems. In addition, wave guide detection allows manipulation of hybridization stringency during detection and thereby reduces DNA chip complexity. It is anticipated that this methodology will provide a powerful tool for diagnostic applications that require rapid cost-effective detection of variations from known sequences. Images Fig. 1 Fig. 2 Fig. 3 PMID:7603999

  12. Fiber-optic detector for real time dosimetry of a micro-planar x-ray beam

    PubMed Central

    Belley, Matthew D.; Stanton, Ian N.; Hadsell, Mike; Ger, Rachel; Langloss, Brian W.; Lu, Jianping; Zhou, Otto; Chang, Sha X.; Therien, Michael J.; Yoshizumi, Terry T.

    2015-01-01

    Purpose: Here, the authors describe a dosimetry measurement technique for microbeam radiation therapy using a nanoparticle-terminated fiber-optic dosimeter (nano-FOD). Methods: The nano-FOD was placed in the center of a 2 cm diameter mouse phantom to measure the deep tissue dose and lateral beam profile of a planar x-ray microbeam. Results: The continuous dose rate at the x-ray microbeam peak measured with the nano-FOD was 1.91 ± 0.06 cGy s−1, a value 2.7% higher than that determined via radiochromic film measurements (1.86 ± 0.15 cGy s−1). The nano-FOD-determined lateral beam full-width half max value of 420 μm exceeded that measured using radiochromic film (320 μm). Due to the 8° angle of the collimated microbeam and resulting volumetric effects within the scintillator, the profile measurements reported here are estimated to achieve a resolution of ∼0.1 mm; however, for a beam angle of 0°, the theoretical resolution would approach the thickness of the scintillator (∼0.01 mm). Conclusions: This work provides proof-of-concept data and demonstrates that the novel nano-FOD device can be used to perform real-time dosimetry in microbeam radiation therapy to measure the continuous dose rate at the x-ray microbeam peak as well as the lateral beam shape. PMID:25832087

  13. A Real-Time Orbit Determination Method for Smooth Transition from Optical Tracking to Laser Ranging of Debris

    PubMed Central

    Li, Bin; Sang, Jizhang; Zhang, Zhongping

    2016-01-01

    A critical requirement to achieve high efficiency of debris laser tracking is to have sufficiently accurate orbit predictions (OP) in both the pointing direction (better than 20 arc seconds) and distance from the tracking station to the debris objects, with the former more important than the latter because of the narrow laser beam. When the two line element (TLE) is used to provide the orbit predictions, the resultant pointing errors are usually on the order of tens to hundreds of arc seconds. In practice, therefore, angular observations of debris objects are first collected using an optical tracking sensor, and then used to guide the laser beam pointing to the objects. The manual guidance may cause interrupts to the laser tracking, and consequently loss of valuable laser tracking data. This paper presents a real-time orbit determination (OD) and prediction method to realize smooth and efficient debris laser tracking. The method uses TLE-computed positions and angles over a short-arc of less than 2 min as observations in an OD process where simplified force models are considered. After the OD convergence, the OP is performed from the last observation epoch to the end of the tracking pass. Simulation and real tracking data processing results show that the pointing prediction errors are usually less than 10″, and the distance errors less than 100 m, therefore, the prediction accuracy is sufficient for the blind laser tracking. PMID:27347958

  14. Real-time pressure monitoring for dynamic control during paper mill operation using fiber optic pressure sensors

    NASA Astrophysics Data System (ADS)

    Fielder, Robert S.; Boyd, Clark; Palmer, Matthew; Eriksen, Oddbjørn

    2006-03-01

    Fiber optic pressure sensors were integrated into the grinding plates of an operational paper pulp mill for real-time monitoring of the pulp grinding process. On-line system monitoring will allow smart, active control of the grinding plates thereby improving the quality and consistency of the pulp produced. Sensors were constructed and calibrated for use in the harsh environment of an operating paper pulp grinder. The sensors were 1.65mm in diameter including titanium housing, and were installed directly into the grooves of the grinding plates. The sensing elements were flush-mounted with the wall and exposed to the wood pulp slurry. Nine sensors were calibrated up to 1000psi. During operation, pressure was sampled at 1.0MHz, and pressure spikes up to 175psi were observed. Pressure pulses measured are due to the relative motion between the grooves and channels on two pulp grinding plates. The consistency, size distribution, and quality of paper pulp exiting from the grinder are directly related to the distance between the channels on the two rotating elements. The pressure pulses produced are also proportional to the distance between channels. Therefore, by monitoring pressure fluctuations, grinding elements can be dynamically controlled thereby producing a "smart mill."

  15. Microvascular anastomosis guidance and evaluation using real-time three-dimensional Fourier-domain Doppler optical coherence tomography

    PubMed Central

    Ibrahim, Zuhaib; Tong, Dedi; Zhu, Shan; Mao, Qi; Pang, John; Andrew Lee, Wei Ping; Brandacher, Gerald; Kang, Jin U.

    2013-01-01

    Abstract. Vascular and microvascular anastomoses are critical components of reconstructive microsurgery, vascular surgery, and transplant surgery. Intraoperative surgical guidance using a surgical imaging modality that provides an in-depth view and three-dimensional (3-D) imaging can potentially improve outcome following both conventional and innovative anastomosis techniques. Objective postoperative imaging of the anastomosed vessel can potentially improve the salvage rate when combined with other clinical assessment tools, such as capillary refill, temperature, blanching, and skin turgor. Compared to other contemporary postoperative monitoring modalities—computed tomography angiograms, magnetic resonance (MR) angiograms, and ultrasound Doppler—optical coherence tomography (OCT) is a noninvasive high-resolution (micron-level), high-speed, 3-D imaging modality that has been adopted widely in biomedical and clinical applications. For the first time, to the best of our knowledge, the feasibility of real-time 3-D phase-resolved Doppler OCT (PRDOCT) as an assisted intra- and postoperative imaging modality for microvascular anastomosis of rodent femoral vessels is demonstrated, which will provide new insights and a potential breakthrough to microvascular and supermicrovascular surgery. PMID:23856833

  16. REAL-TIME OPTICAL SPECTROSCOPY OF VACUUM ULTRAVIOLET IRRADIATED PYRENE:H{sub 2}O INTERSTELLAR ICE

    SciTech Connect

    Bouwman, J.; Paardekooper, D. M.; Cuppen, H. M.; Linnartz, H.; Allamandola, L. J.

    2009-07-20

    This paper describes a near-UV/VIS study of a pyrene:H{sub 2}O interstellar ice analogue at 10 K using optical absorption spectroscopy. A new experimental approach makes it possible to irradiate the sample with vacuum ultraviolet (VUV) light (7-10.5 eV) while simultaneously recording spectra in the 240-1000 nm range with subsecond time resolution. Both spectroscopic and dynamic information on VUV processed ices are obtained in this way. This provides a powerful tool to follow, in situ and in real time, the photophysical and photochemical processes induced by VUV irradiation of a polycyclic aromatic hydrocarbon containing inter- and circumstellar ice analogue. Results on the VUV photolysis of a prototype sample-strongly diluted pyrene in H{sub 2}O ice-are presented. In addition to the pyrene cation (Py{sup +}), other products-hydroxypyrene (PyOH), possibly hydroxypyrene cation (PyOH{sup +}), and pyrene/pyrenolate anion (Py{sup -}/PyO{sup -})-are observed. It is found that the charge remains localized in the ice, also after the VUV irradiation is stopped. The astrochemical implications and observational constraints are discussed.

  17. Real-time modeling and online filtering of the stochastic error in a fiber optic current transducer

    NASA Astrophysics Data System (ADS)

    Wang, Lihui; Wei, Guangjin; Zhu, Yunan; Liu, Jian; Tian, Zhengqi

    2016-10-01

    The stochastic error characteristics of a fiber optic current transducer (FOCT) influence the relay protection, electric-energy metering, and other devices in the spacer layer. Real-time modeling and online filtering of the FOCT’s stochastic error tends to be an effective method for improving the measurement accuracy of the FOCT. This paper first pretreats and inspects the FOCT data, statistically. Then, the model order is set by the AIC principle to establish an ARMA (2,1) model and model’s applicability is tested. Finally, a Kalman filter is adopted to reduce the noise in the FOCT data. The results of the experiment and the simulation demonstrate that there is a notable decrease in the stochastic error after time series modeling and Kalman filtering. Besides, the mean-variance is decreased by two orders. All the stochastic error coefficients are decreased by the total variance method; the BI is decreased by 41.4%, the RRW is decreased by 67.5%, and the RR is decreased by 53.4%. Consequently, the method can reduce the stochastic error and improve the measurement accuracy of the FOCT, effectively.

  18. Implementations of adaptive associative optical computing elements

    NASA Astrophysics Data System (ADS)

    Fisher, Arthur D.; Lee, John N.; Fukuda, Robert C.

    1986-01-01

    The present optical implementations for heteroassociative memory modules, which are capable of real time adaptive learning, are pertinent to the eventual construction of large, multimodule associative/neural network architectures that can consider problems in the acquisition, transformation, matching/recognition, and manipulation of large amounts of data in parallel. These modules offer such performance features as convergence to the least-squares-optimum pseudoinverse association, accumulative and gated learning, forgetfulness of unused associations, resistance to dynamic-range saturation, and compensation of optical system aberrations. Optics uniquely furnish the massive parallel interconnection paths required to cascade and interconnect a number of modules to form the more sophisticated multiple module architectures.

  19. An in-situ real-time optical fiber sensor based on surface plasmon resonance for monitoring the growth of TiO2 thin films.

    PubMed

    Tsao, Yu-Chia; Tsai, Woo-Hu; Shih, Wen-Ching; Wu, Mu-Shiang

    2013-07-23

    An optical fiber sensor based on surface plasmon resonance (SPR) is proposed for monitoring the thickness of deposited nano-thin films. A side-polished multimode SPR optical fiber sensor with an 850 nm-LD is used as the transducing element for real-time monitoring of the deposited TiO2 thin films. The SPR optical fiber sensor was installed in the TiO2 sputtering system in order to measure the thickness of the deposited sample during TiO2 deposition. The SPR response declined in real-time in relation to the growth of the thickness of the TiO2 thin film. Our results show the same trend of the SPR response in real-time and in spectra taken before and after deposition. The SPR transmitted intensity changes by approximately 18.76% corresponding to 50 nm of deposited TiO2 thin film. We have shown that optical fiber sensors utilizing SPR have the potential for real-time monitoring of the SPR technology of nanometer film thickness. The compact size of the SPR fiber sensor enables it to be positioned inside the deposition chamber, and it could thus measure the film thickness directly in real-time. This technology also has potential application for monitoring the deposition of other materials. Moreover, in-situ real-time SPR optical fiber sensor technology is in inexpensive, disposable technique that has anti-interference properties, and the potential to enable on-line monitoring and monitoring of organic coatings.

  20. SU-E-T-672: Real-Time In Vivo Dosimeters Using LiPCDA and Optical Fibers

    SciTech Connect

    Rink, A; Jaffray, DA; Croteau, A; Caron, S; Mermut, O

    2015-06-15

    Purpose: To investigate dosimeter prototypes made with lithium pentacosa-10,12-diynoate (LiPCDA, the material used in GafChromic EBT films) and optical fibers for their suitability in real-time in vivo measurements. Methods: The prototypes, made with 500 µm plastic optical fibers and 8.5 µm thickness of LiPCDA at fiber tip, were irradiated with a 6 MV beam. To investigate the efficacy of pre-irradiation calibration, the probes were irradiated to 2 Gy twice, with 5 minutes in between. Net optical density values (netOD) around the main absorbance peak were compared, and effect of correcting second measurement by the first was assessed. Ageing was assessed by irradiating two prototypes to 2 Gy and comparing the netOD to that obtained for 15 prototypes from the same batch 12–14 months earlier. To measure angular dependence, the probes were pre-irradiated with beam perpendicular to fiber axis and then, 5 minutes later either perpendicular or parallel to fiber axes. The thickness-corrected netOD measurements were compared. Results: Standard deviation of netOD for probes of the same batch was measured to be 5–6%. When netOD was corrected for material thickness by using results from the first irradiation, the standard deviation decreased to 1.3%. This was comparable to the uncertainty in measurements observed with a single probe and is attributed to variations in light output, spectrometer noise and splitter-to-probe connection variations. Comparison of netOD values obtained a year apart failed to illustrate statistically significant decrease in sensitivity due to ageing (0.38 ± 0.03 and 0.3656 ± 0.0003). NetOD measured with MV beam parallel to fiber was within error of netOD measured with MV beam perpendicular to fiber. Conclusion: Current prototype construction allows for shelf life of at least one year. With material thickness corrected for, the prototypes can measure dose with an uncertainty below 2% at a given energy and dose rate. This work has been funded by

  1. Fiber-optic detector for real time dosimetry of a micro-planar x-ray beam

    SciTech Connect

    Belley, Matthew D.; Stanton, Ian N.; Langloss, Brian W.; Therien, Michael J.; Hadsell, Mike; Ger, Rachel; Lu, Jianping; Zhou, Otto; Chang, Sha X.; Yoshizumi, Terry T.

    2015-04-15

    Purpose: Here, the authors describe a dosimetry measurement technique for microbeam radiation therapy using a nanoparticle-terminated fiber-optic dosimeter (nano-FOD). Methods: The nano-FOD was placed in the center of a 2 cm diameter mouse phantom to measure the deep tissue dose and lateral beam profile of a planar x-ray microbeam. Results: The continuous dose rate at the x-ray microbeam peak measured with the nano-FOD was 1.91 ± 0.06 cGy s{sup −1}, a value 2.7% higher than that determined via radiochromic film measurements (1.86 ± 0.15 cGy s{sup −1}). The nano-FOD-determined lateral beam full-width half max value of 420 μm exceeded that measured using radiochromic film (320 μm). Due to the 8° angle of the collimated microbeam and resulting volumetric effects within the scintillator, the profile measurements reported here are estimated to achieve a resolution of ∼0.1 mm; however, for a beam angle of 0°, the theoretical resolution would approach the thickness of the scintillator (∼0.01 mm). Conclusions: This work provides proof-of-concept data and demonstrates that the novel nano-FOD device can be used to perform real-time dosimetry in microbeam radiation therapy to measure the continuous dose rate at the x-ray microbeam peak as well as the lateral beam shape.

  2. Real-Time Adaptive Lossless Hyperspectral Image Compression using CCSDS on Parallel GPGPU and Multicore Processor Systems

    NASA Technical Reports Server (NTRS)

    Hopson, Ben; Benkrid, Khaled; Keymeulen, Didier; Aranki, Nazeeh; Klimesh, Matt; Kiely, Aaron

    2012-01-01

    The proposed CCSDS (Consultative Committee for Space Data Systems) Lossless Hyperspectral Image Compression Algorithm was designed to facilitate a fast hardware implementation. This paper analyses that algorithm with regard to available parallelism and describes fast parallel implementations in software for GPGPU and Multicore CPU architectures. We show that careful software implementation, using hardware acceleration in the form of GPGPUs or even just multicore processors, can exceed the performance of existing hardware and software implementations by up to 11x and break the real-time barrier for the first time for a typical test application.

  3. Three Experiments Examining the Use of Electroencephalogram,Event-Related Potentials, and Heart-Rate Variability for Real-Time Human-Centered Adaptive Automation Design

    NASA Technical Reports Server (NTRS)

    Prinzel, Lawrence J., III; Parasuraman, Raja; Freeman, Frederick G.; Scerbo, Mark W.; Mikulka, Peter J.; Pope, Alan T.

    2003-01-01

    Adaptive automation represents an advanced form of human-centered automation design. The approach to automation provides for real-time and model-based assessments of human-automation interaction, determines whether the human has entered into a hazardous state of awareness and then modulates the task environment to keep the operator in-the-loop , while maintaining an optimal state of task engagement and mental alertness. Because adaptive automation has not matured, numerous challenges remain, including what the criteria are, for determining when adaptive aiding and adaptive function allocation should take place. Human factors experts in the area have suggested a number of measures including the use of psychophysiology. This NASA Technical Paper reports on three experiments that examined the psychophysiological measures of event-related potentials, electroencephalogram, and heart-rate variability for real-time adaptive automation. The results of the experiments confirm the efficacy of these measures for use in both a developmental and operational role for adaptive automation design. The implications of these results and future directions for psychophysiology and human-centered automation design are discussed.

  4. A long-time, high spatiotemporal resolution optical recording system for membrane potential activity via real-time writing to the hard disk.

    PubMed

    Hirota, Akihiko; Ito, Shin-ichi

    2006-06-01

    Using real-time hard disk recording, we have developed an optical system for the long-duration detection of changes in membrane potential from 1,020 sites with a high temporal resolution. The signal-to-noise ratio was sufficient for analyzing the spreading pattern of excitatory waves in frog atria in a single sweep.

  5. Adaption of SYBR Green-based reagent kit for real-time PCR quantitation of GC-rich DNA.

    PubMed

    Chang, G J; Seyfert, H M; Shen, X Z

    2015-07-28

    In the mammalian genome, approximately 50% of all genes are controlled by promoters with high GC contents. Analyzing the epigenetic mechanisms regulating their expression is difficult. Hence, we examined a method for stable quantification of such GC-rich DNA sequences. Quantification of DNA during real-time PCR is often based on reagent kits containing the fluorescent dye SYBR Green. However, these ready-made kits may not be suitable for amplifying DNA samples with a high GC content (>70%). DNA segments with eccentric GC contents are frequently found in proximal promoter areas, and their quantification may be necessary in chromatin accessibility by real-time polymerase chain reaction or chromatin immunoprecipitation analyses of epigenetic mechanisms of gene regulation. We therefore optimized the SYBR Green I FastStart reaction system by supplementing the system with dimethyl sulfoxide, betaine, and increased DNA polymerase content. Here, we describe the development of the assay and demonstrate its effectiveness for two different DNA templates, showing that these modifications allow for the reliable amplification and quantification of DNA with GC contents exceeding >70% using the LightCycler instrument.

  6. Adaptive Optics at Lawrence Livermore National Laboratory

    SciTech Connect

    Gavel, D T

    2003-03-10

    Adaptive optics enables high resolution imaging through the atmospheric by correcting for the turbulent air's aberrations to the light waves passing through it. The Lawrence Livermore National Laboratory for a number of years has been at the forefront of applying adaptive optics technology to astronomy on the world's largest astronomical telescopes, in particular at the Keck 10-meter telescope on Mauna Kea, Hawaii. The technology includes the development of high-speed electrically driven deformable mirrors, high-speed low-noise CCD sensors, and real-time wavefront reconstruction and control hardware. Adaptive optics finds applications in many other areas where light beams pass through aberrating media and must be corrected to maintain diffraction-limited performance. We describe systems and results in astronomy, medicine (vision science), and horizontal path imaging, all active programs in our group.

  7. Optical PEBBLE nanosensors and fiber optic sensors for real-time intracellular imaging and analysis of magnesium and oxygen

    NASA Astrophysics Data System (ADS)

    Park, Edwin J.

    A highly selective magnesium fluorescent optical nanosensor, made possible by PEBBLE (Probe Encapsulated By Biologically Localized Embedding) technology is presented. Ratiometric sensors were prepared, via a microemulsion polymerization process, by co-immobilizing a highly selective magnesium dye with a reference dye inside a polymer matrix. The resultant spherical sensors are ˜40 nm in diameter. Several dyes were investigated with coumarin 343 (C343) providing the best selectivity towards Mg2+ vs. ions such as Ca 2+, Na+, and K+. The dynamic range of the sensors was 1 to 30 mM (linear from 1 to 10 mM) with a response time of less than 4 s. The fully reversible sensors exhibit minimal leaching and photobleaching. In vitro intracellular changes in Mg2+ concentration were monitored in C6 Glioma cells. Preliminary experiments demonstrated the capability of these sensors thus they were used to investigate the PhoP/Q transmembrane protein system on the internal membrane of salmonella . The sensors were used to monitor the magnesium levels inside salmonella containing vacuoles. Previously believed hypotheses on changes in magnesium concentration are challenged with the newly obtained results. The selectivity for Mg2+ along with the biocompatibility of the matrix of these sensors provides a new and reliable tool for intracellular magnesium measurements. A second sensor platform was developed for the detection of intracellular dissolved oxygen, using a fiber optic probe. The design and fabrication of an oxygen fiber optic sensor based on the fluorescence quenching properties of the oxygen sensitive platinum (II) octaethylporphine ketone (PtOEPK) is presented. Octaethyl porphyrin (OEP) or bodipy maleimide 577/618, was also entrapped, as a reference dye, in a polyvinyl chloride matrix including the plasticizing agent bis 2-ethylhexyl sebacate (DOS). The multi mode fibers were pulled down to submicron dimensions and a dip coating procedure was used to apply the sensing

  8. In Situ Analysis of a High-Temperature Cure Reaction in Real Time Using Modulated Fiber-Optic FT-Raman Spectroscopy

    NASA Technical Reports Server (NTRS)

    Aust, Jeffrey F.; Cooper, John B.; Wise, Kent L.; Jensen, Brian J.

    1999-01-01

    The vibrational spectrum of a high-temperature (330 C) polymerization reaction was successfully monitored in real time with the use of a modulated fiber-optic Fourier transform (FT)-Raman spectrometer. A phenylethynyl-terminated monomer was cured, and spectral evidence for two different reaction products was acquired. The products are a conjugated polyene chain and a cyclized trimer. This is the first report describing the use of FT-Raman spectroscopy to monitor a high temperature (greater than 250 C) reaction in real time.

  9. Robust Wiener filtering for Adaptive Optics

    SciTech Connect

    Poyneer, L A

    2004-06-17

    In many applications of optical systems, the observed field in the pupil plane has a non-uniform phase component. This deviation of the phase of the field from uniform is called a phase aberration. In imaging systems this aberration will degrade the quality of the images. In the case of a large astronomical telescope, random fluctuations in the atmosphere lead to significant distortion. These time-varying distortions can be corrected using an Adaptive Optics (AO) system, which is a real-time control system composed of optical, mechanical and computational parts. Adaptive optics is also applicable to problems in vision science, laser propagation and communication. For a high-level overview, consult this web site. For an in-depth treatment of the astronomical case, consult these books.

  10. Thin nearly wireless adaptive optical device

    NASA Technical Reports Server (NTRS)

    Knowles, Gareth J. (Inventor); Hughes, Eli (Inventor)

    2009-01-01

    A thin nearly wireless adaptive optical device capable of dynamically modulating the shape of a mirror in real time to compensate for atmospheric distortions and/or variations along an optical material is provided. The device includes an optical layer, a substrate, at least one electronic circuit layer with nearly wireless architecture, an array of actuators, power electronic switches, a reactive force element, and a digital controller. Actuators are aligned so that each axis of expansion and contraction intersects both substrate and reactive force element. Electronics layer with nearly wireless architecture, power electronic switches, and digital controller are provided within a thin-film substrate. The size and weight of the adaptive optical device is solely dominated by the size of the actuator elements rather than by the power distribution system.

  11. Thin, nearly wireless adaptive optical device

    NASA Technical Reports Server (NTRS)

    Knowles, Gareth (Inventor); Hughes, Eli (Inventor)

    2008-01-01

    A thin, nearly wireless adaptive optical device capable of dynamically modulating the shape of a mirror in real time to compensate for atmospheric distortions and/or variations along an optical material is provided. The device includes an optical layer, a substrate, at least one electronic circuit layer with nearly wireless architecture, an array of actuators, power electronic switches, a reactive force element, and a digital controller. Actuators are aligned so that each axis of expansion and contraction intersects both substrate and reactive force element. Electronics layer with nearly wireless architecture, power electronic switches, and digital controller are provided within a thin-film substrate. The size and weight of the adaptive optical device is solely dominated by the size of the actuator elements rather than by the power distribution system.

  12. Thin, nearly wireless adaptive optical device

    NASA Technical Reports Server (NTRS)

    Knowles, Gareth (Inventor); Hughes, Eli (Inventor)

    2007-01-01

    A thin, nearly wireless adaptive optical device capable of dynamically modulating the shape of a mirror in real time to compensate for atmospheric distortions and/or variations along an optical material is provided. The device includes an optical layer, a substrate, at least one electronic circuit layer with nearly wireless architecture, an array of actuators, power electronic switches, a reactive force element, and a digital controller. Actuators are aligned so that each axis of expansion and contraction intersects both substrate and reactive force element. Electronics layer with nearly wireless architecture, power electronic switches, and digital controller are provided within a thin-film substrate. The size and weight of the adaptive optical device is solely dominated by the size of the actuator elements rather than by the power distribution system.

  13. Real-time terahertz wave imaging by nonlinear optical frequency up-conversion in a 4-dimethylamino-N'-methyl-4'-stilbazolium tosylate crystal

    NASA Astrophysics Data System (ADS)

    Fan, Shuzhen; Qi, Feng; Notake, Takashi; Nawata, Kouji; Matsukawa, Takeshi; Takida, Yuma; Minamide, Hiroaki

    2014-03-01

    Real-time terahertz (THz) wave imaging has wide applications in areas such as security, industry, biology, medicine, pharmacy, and arts. In this letter, we report on real-time room-temperature THz imaging by nonlinear optical frequency up-conversion in organic 4-dimethylamino-N'-methyl-4'-stilbazolium tosylate crystal. The active projection-imaging system consisted of (1) THz wave generation, (2) THz-near-infrared hybrid optics, (3) THz wave up-conversion, and (4) an InGaAs camera working at 60 frames per second. The pumping laser system consisted of two optical parametric oscillators pumped by a nano-second frequency-doubled Nd:YAG laser. THz-wave images of handmade samples at 19.3 THz were taken, and videos of a sample moving and a ruler stuck with a black polyethylene film moving were supplied online to show real-time ability. Thanks to the high speed and high responsivity of this technology, real-time THz imaging with a higher signal-to-noise ratio than a commercially available THz micro-bolometer camera was proven to be feasible. By changing the phase-matching condition, i.e., by changing the wavelength of the pumping laser, we suggest THz imaging with a narrow THz frequency band of interest in a wide range from approximately 2 to 30 THz is possible.

  14. Solar tomography adaptive optics.

    PubMed

    Ren, Deqing; Zhu, Yongtian; Zhang, Xi; Dou, Jiangpei; Zhao, Gang

    2014-03-10

    Conventional solar adaptive optics uses one deformable mirror (DM) and one guide star for wave-front sensing, which seriously limits high-resolution imaging over a large field of view (FOV). Recent progress toward multiconjugate adaptive optics indicates that atmosphere turbulence induced wave-front distortion at different altitudes can be reconstructed by using multiple guide stars. To maximize the performance over a large FOV, we propose a solar tomography adaptive optics (TAO) system that uses tomographic wave-front information and uses one DM. We show that by fully taking advantage of the knowledge of three-dimensional wave-front distribution, a classical solar adaptive optics with one DM can provide an extra performance gain for high-resolution imaging over a large FOV in the near infrared. The TAO will allow existing one-deformable-mirror solar adaptive optics to deliver better performance over a large FOV for high-resolution magnetic field investigation, where solar activities occur in a two-dimensional field up to 60'', and where the near infrared is superior to the visible in terms of magnetic field sensitivity.

  15. Solar Adaptive Optics.

    PubMed

    Rimmele, Thomas R; Marino, Jose

    Adaptive optics (AO) has become an indispensable tool at ground-based solar telescopes. AO enables the ground-based observer to overcome the adverse effects of atmospheric seeing and obtain diffraction limited observations. Over the last decade adaptive optics systems have been deployed at major ground-based solar telescopes and revitalized ground-based solar astronomy. The relatively small aperture of solar telescopes and the bright source make solar AO possible for visible wavelengths where the majority of solar observations are still performed. Solar AO systems enable diffraction limited observations of the Sun for a significant fraction of the available observing time at ground-based solar telescopes, which often have a larger aperture than equivalent space based observatories, such as HINODE. New ground breaking scientific results have been achieved with solar adaptive optics and this trend continues. New large aperture telescopes are currently being deployed or are under construction. With the aid of solar AO these telescopes will obtain observations of the highly structured and dynamic solar atmosphere with unprecedented resolution. This paper reviews solar adaptive optics techniques and summarizes the recent progress in the field of solar adaptive optics. An outlook to future solar AO developments, including a discussion of Multi-Conjugate AO (MCAO) and Ground-Layer AO (GLAO) will be given.

  16. The ERIS adaptive optics system

    NASA Astrophysics Data System (ADS)

    Marchetti, Enrico; Fedrigo, Enrico; Le Louarn, Miska; Madec, Pierre-Yves; Soenke, Christian; Brast, Roland; Conzelmann, Ralf; Delabre, Bernard; Duchateau, Michel; Frank, Christoph; Klein, Barbara; Amico, Paola; Hubin, Norbert; Esposito, Simone; Antichi, Jacopo; Carbonaro, Luca; Puglisi, Alfio; Quirós-Pacheco, Fernando; Riccardi, Armando; Xompero, Marco

    2014-07-01

    The Enhanced Resolution Imager and Spectrograph (ERIS) is the new Adaptive Optics based instrument for ESO's VLT aiming at replacing NACO and SINFONI to form a single compact facility with AO fed imaging and integral field unit spectroscopic scientific channels. ERIS completes the instrument suite at the VLT adaptive telescope. In particular it is equipped with a versatile AO system that delivers up to 95% Strehl correction in K band for science observations up to 5 micron It comprises high order NGS and LGS correction enabling the observation from exoplanets to distant galaxies with a large sky coverage thanks to the coupling of the LGS WFS with the high sensitivity of its visible WFS and the capability to observe in dust embedded environment thanks to its IR low order WFS. ERIS will be installed at the Cassegrain focus of the VLT unit hosting the Adaptive Optics Facility (AOF). The wavefront correction is provided by the AOF deformable secondary mirror while the Laser Guide Star is provided by one of the four launch units of the 4 Laser Guide Star Facility for the AOF. The overall layout of the ERIS AO system is extremely compact and highly optimized: the SPIFFI spectrograph is fed directly by the Cassegrain focus and both the NIX's (IR imager) and SPIFFI's entrance windows work as visible/infrared dichroics. In this paper we describe the concept of the ERIS AO system in detail, starting from the requirements and going through the estimated performance, the opto-mechanical design and the Real-Time Computer design.

  17. An adaptive-gain complementary filter for real-time human motion tracking with MARG sensors in free-living environments.

    PubMed

    Tian, Ya; Wei, Hongxing; Tan, Jindong

    2013-03-01

    High-resolution, real-time data obtained by human motion tracking systems can be used for gait analysis, which helps better understanding the cause of many diseases for more effective treatments, such as rehabilitation for outpatients or recovery from lost motor functions after a stroke. In order to achieve real-time ambulatory human motion tracking with low-cost MARG (magnetic, angular rate, and gravity) sensors, a computationally efficient and robust algorithm for orientation estimation is critical. This paper presents an analytically derived method for an adaptive-gain complementary filter based on the convergence rate from the Gauss-Newton optimization algorithm (GNA) and the divergence rate from the gyroscope, which is referred as adaptive-gain orientation filter (AGOF) in this paper. The AGOF has the advantages of one iteration calculation to reduce the computing load and accurate estimation of gyroscope measurement error. Moreover, for handling magnetic distortions especially in indoor environments and movements with excessive acceleration, adaptive measurement vectors and a reference vector for earth's magnetic field selection schemes are introduced to help the GNA find more accurate direction of gyroscope error. The features of this approach include the accurate estimation of the gyroscope bias to correct the instantaneous gyroscope measurements and robust estimation in conditions of fast motions and magnetic distortions. Experimental results are presented to verify the performance of the proposed method, which shows better accuracy of orientation estimation than several well-known methods.

  18. Real-Time Optical Flux Limits from Gamma-Ray Bursts Measured by the Gamma-Ray Optical Counterpart Search Experiment

    NASA Technical Reports Server (NTRS)

    Park, H. S. (Editor); Ables, E. (Editor); Band, D. L. (Editor); Barthelmy, S. D. (Editor); Bionta, R. M. (Editor); Butterworth, P. S. (Editor); Cline, T. L. (Editor); Ferguson, D. H. (Editor); Fishman, G. J. (Editor); Gehrels, N. (Editor); Hurley, K. (Editor); Kouveliotou, C. (Editor); Lee, B. C. (Editor); Meegan, C. A. (Editor); Ott, L. L. (Editor); Parker, E. L. (Editor)

    1997-01-01

    The Gamma-Ray Optical Counterpart Search Experiment presents new experimental upper limits on the optical flux from gamma-ray bursts (GRBs). Our experiment consisted of a fully automated very wide-field opto-electronic detection system that imaged locations of GRBs within a few seconds of receiving trigger signals provided by BATSE's real-time burst coordinate distribution network. The experiment acquired 3800 observing hours, recording 22 gamma-ray burst triggers within approx. 30 s of the start of the burst event. Some of these bursts were imaged while gamma-ray radiation was being detected by BATSE. We identified no optical counterparts associated with gamma-ray bursts among these events at the mV approx. 7.0-8.3 sensitivity level. We find the ratio of the upper limit to the V-band optical flux, F nu, to the gamma-ray fluence, Phi gamma, from these data to be 1 x 10(exp-18) less than F nu Phi gamma less than 2 x 10(exp -16).

  19. FPGA-accelerated adaptive optics wavefront control

    NASA Astrophysics Data System (ADS)

    Mauch, S.; Reger, J.; Reinlein, C.; Appelfelder, M.; Goy, M.; Beckert, E.; Tünnermann, A.

    2014-03-01

    The speed of real-time adaptive optical systems is primarily restricted by the data processing hardware and computational aspects. Furthermore, the application of mirror layouts with increasing numbers of actuators reduces the bandwidth (speed) of the system and, thus, the number of applicable control algorithms. This burden turns out a key-impediment for deformable mirrors with continuous mirror surface and highly coupled actuator influence functions. In this regard, specialized hardware is necessary for high performance real-time control applications. Our approach to overcome this challenge is an adaptive optics system based on a Shack-Hartmann wavefront sensor (SHWFS) with a CameraLink interface. The data processing is based on a high performance Intel Core i7 Quadcore hard real-time Linux system. Employing a Xilinx Kintex-7 FPGA, an own developed PCie card is outlined in order to accelerate the analysis of a Shack-Hartmann Wavefront Sensor. A recently developed real-time capable spot detection algorithm evaluates the wavefront. The main features of the presented system are the reduction of latency and the acceleration of computation For example, matrix multiplications which in general are of complexity O(n3 are accelerated by using the DSP48 slices of the field-programmable gate array (FPGA) as well as a novel hardware implementation of the SHWFS algorithm. Further benefits are the Streaming SIMD Extensions (SSE) which intensively use the parallelization capability of the processor for further reducing the latency and increasing the bandwidth of the closed-loop. Due to this approach, up to 64 actuators of a deformable mirror can be handled and controlled without noticeable restriction from computational burdens.

  20. Real time on-chip sequential adaptive principal component analysis for data feature extraction and image compression

    NASA Technical Reports Server (NTRS)

    Duong, T. A.

    2004-01-01

    In this paper, we present a new, simple, and optimized hardware architecture sequential learning technique for adaptive Principle Component Analysis (PCA) which will help optimize the hardware implementation in VLSI and to overcome the difficulties of the traditional gradient descent in learning convergence and hardware implementation.

  1. In-vivo, real-time cross-sectional images of retina using a GPU enhanced master slave optical coherence tomography system

    NASA Astrophysics Data System (ADS)

    Bradu, Adrian; Kapinchev, Konstantin; Barnes, Frederick; Podoleanu, Adrian

    2016-03-01

    In our previous reports we demonstrated a novel Fourier domain optical coherence tomography method, Master Slave optical coherence tomography (MS-OCT), that does not require resampling of data and can deliver en-face images from several depths simultaneously. While ideally suited for delivering information from a selected depth, the MS-OCT has been so far inferior to the conventional FFT based OCT in terms of time of producing cross section images. Here, we demonstrate that by taking advantage of the parallel processing capabilities offered by the MS-OCT method, cross-sectional OCT images of the human retina can be produced in real-time by assembling several T-scans from different depths. We analyze the conditions that ensure a real-time B-scan imaging operation, and demonstrate in-vivo real-time images from human fovea and the optic nerve, of comparable resolution and sensitivity to those produced using the traditional Fourier domain based method.

  2. A real-time regional adaptive exposure method for saving dose-area product in x-ray fluoroscopy

    SciTech Connect

    Burion, Steve; Funk, Tobias; Speidel, Michael A.

    2013-05-15

    Purpose: Reduction of radiation dose in x-ray imaging has been recognized as a high priority in the medical community. Here the authors show that a regional adaptive exposure method can reduce dose-area product (DAP) in x-ray fluoroscopy. The authors' method is particularly geared toward providing dose savings for the pediatric population. Methods: The scanning beam digital x-ray system uses a large-area x-ray source with 8000 focal spots in combination with a small photon-counting detector. An imaging frame is obtained by acquiring and reconstructing up to 8000 detector images, each viewing only a small portion of the patient. Regional adaptive exposure was implemented by varying the exposure of the detector images depending on the local opacity of the object. A family of phantoms ranging in size from infant to obese adult was imaged in anteroposterior view with and without adaptive exposure. The DAP delivered to each phantom was measured in each case, and noise performance was compared by generating noise arrays to represent regional noise in the images. These noise arrays were generated by dividing the image into regions of about 6 mm{sup 2}, calculating the relative noise in each region, and placing the relative noise value of each region in a one-dimensional array (noise array) sorted from highest to lowest. Dose-area product savings were calculated as the difference between the ratio of DAP with adaptive exposure to DAP without adaptive exposure. The authors modified this value by a correction factor that matches the noise arrays where relative noise is the highest to report a final dose-area product savings. Results: The average dose-area product saving across the phantom family was (42 {+-} 8)% with the highest dose-area product saving in the child-sized phantom (50%) and the lowest in the phantom mimicking an obese adult (23%). Conclusions: Phantom measurements indicate that a regional adaptive exposure method can produce large DAP savings without

  3. Advanced Adaptive Optics Control Techniques

    DTIC Science & Technology

    1979-01-01

    Optimal estimation and control methods for high energy laser adaptive optics systems are described. Three system types are examined: Active...the adaptive optics approaches and potential system implementations are recommended.

  4. Real-time measurement of the vaginal pressure profile using an optical-fiber-based instrumented speculum

    NASA Astrophysics Data System (ADS)

    Parkinson, Luke A.; Gargett, Caroline E.; Young, Natharnia; Rosamilia, Anna; Vashi, Aditya V.; Werkmeister, Jerome A.; Papageorgiou, Anthony W.; Arkwright, John W.

    2016-12-01

    Pelvic organ prolapse (POP) occurs when changes to the pelvic organ support structures cause descent or herniation of the pelvic organs into the vagina. Clinical evaluation of POP is a series of manual measurements known as the pelvic organ prolapse quantification (POP-Q) score. However, it fails to identify the mechanism causing POP and relies on the skills of the practitioner. We report on a modified vaginal speculum incorporating a double-helix fiber-Bragg grating structure for distributed pressure measurements along the length of the vagina and include preliminary data in an ovine model of prolapse. Vaginal pressure profiles were recorded at 10 Hz as the speculum was dilated incrementally up to 20 mm. At 10-mm dilation, nulliparous sheep showed higher mean pressures (102±46 mmHg) than parous sheep (39±23 mmHg) (P=0.02), attributable largely to the proximal (cervical) end of the vagina. In addition to overall pressure variations, we observed a difference in the distribution of pressure that related to POP-Q measurements adapted for the ovine anatomy, showing increased tissue laxity in the upper anterior vagina for parous ewes. We demonstrate the utility of the fiber-optic instrumented speculum for rapid distributed measurement of vaginal support.

  5. Advanced Adaptive Optics Technology Development

    SciTech Connect

    Olivier, S

    2001-09-18

    The NSF Center for Adaptive Optics (CfAO) is supporting research on advanced adaptive optics technologies. CfAO research activities include development and characterization of micro-electro-mechanical systems (MEMS) deformable mirror (DM) technology, as well as development and characterization of high-resolution adaptive optics systems using liquid crystal (LC) spatial light modulator (SLM) technology. This paper presents an overview of the CfAO advanced adaptive optics technology development activities including current status and future plans.

  6. Optics based signal processing methods for intraoperative blood vessel detection and quantification in real time (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Chaturvedi, Amal; Shukair, Shetha A.; Le Rolland, Paul; Vijayvergia, Mayank; Subramanian, Hariharan; Gunn, Jonathan W.

    2016-03-01

    Minimally invasive operations require surgeons to make difficult cuts to blood vessels and other tissues with impaired tactile and visual feedback. This leads to inadvertent cuts to blood vessels hidden beneath tissue, causing serious health risks to patients and a non-reimbursable financial burden to hospitals. Intraoperative imaging technologies have been developed, but these expensive systems can be cumbersome and provide only a high-level view of blood vessel networks. In this research, we propose a lean reflectance-based system, comprised of a dual wavelength LED, photodiode, and novel signal processing algorithms for rapid vessel characterization. Since this system takes advantage of the inherent pulsatile light absorption characteristics of blood vessels, no contrast agent is required for its ability to detect the presence of a blood vessel buried deep inside any tissue type (up to a cm) in real time. Once a vessel is detected, the system is able to estimate the distance of the vessel from the probe and the diameter size of the vessel (with a resolution of ~2mm), as well as delineate the type of tissue surrounding the vessel. The system is low-cost, functions in real-time, and could be mounted on already existing surgical tools, such as Kittner dissectors or laparoscopic suction irrigation cannulae. Having been successfully validated ex vivo, this technology will next be tested in a live porcine study and eventually in clinical trials.

  7. Adaptive optics ophthalmoscopy.

    PubMed

    Roorda, Austin; Duncan, Jacque L

    2015-11-01

    This review starts with a brief history and description of adaptive optics (AO) technology, followed by a showcase of the latest capabilities of AO systems for imaging the human retina and an extensive review of the literature on where AO is being used clinically. The review concludes with a discussion on future directions and guidance on usage and interpretation of images from AO systems for the eye.

  8. On the possibility of producing true real-time retinal cross-sectional images using a graphics processing unit enhanced master-slave optical coherence tomography system

    NASA Astrophysics Data System (ADS)

    Bradu, Adrian; Kapinchev, Konstantin; Barnes, Frederick; Podoleanu, Adrian

    2015-07-01

    In a previous report, we demonstrated master-slave optical coherence tomography (MS-OCT), an OCT method that does not need resampling of data and can be used to deliver en face images from several depths simultaneously. In a separate report, we have also demonstrated MS-OCT's capability of producing cross-sectional images of a quality similar to those provided by the traditional Fourier domain (FD) OCT technique, but at a much slower rate. Here, we demonstrate that by taking advantage of the parallel processing capabilities offered by the MS-OCT method, cross-sectional OCT images of the human retina can be produced in real time. We analyze the conditions that ensure a true real-time B-scan imaging operation and demonstrate in vivo real-time images from human fovea and the optic nerve, with resolution and sensitivity comparable to those produced using the traditional FD-based method, however, without the need of data resampling.

  9. Holographic Adaptive Optics

    NASA Astrophysics Data System (ADS)

    Andersen, G.

    For the last two decades adaptive optics has been used as a technique for correcting imaging applications and directed energy/laser targeting and laser communications systems affected by atmospheric turbulence. Typically these systems are bulky and limited to <10 kHz due to large computing overhead and limited photon efficiencies. Moreover most use zonal wavefront sensors which cannot easily handle extreme scintillation or unexpected obscuration of a pre-set aperture. Here we present a compact, lightweight adaptive optics system with the potential to operate at speeds of MHz. The system utilizes a hologram to perform an all-optical wavefront analysis that removes the need for any computer. Finally, the sensing is made on a modal basis so it is largely insensitive to scintillation and obscuration. We have constructed a prototype device and will present experimental results from our research. The holographic adaptive optics system begins with the creation of a multiplexed hologram. This hologram is created by recording the maximum and minimum response functions of every actuator in the deformable mirror against a unique focused reference beam. When a wavefront of some arbitrary phase is incident on the processed hologram, a number of focal spots are created -- one pair for each actuator in the DM. The absolute phase error at each particular actuator location is simply related to the ratio of the intensity of each pair of spots. In this way we can use an array of photodetectors to give a direct readout of phase error without the need for any calculations. The advantages of holographic adaptive optics are many. To begin with, the measurement of phase error is made all optically, so the wavefront sensor directly controls the actuators in the DM without any computers. Using fast, photon counting photodetectors allows for closed loop correction limited only by the speed of the deformable mirror which in the case of MEMS devices can be 100 kHz or more. All this can be

  10. Back-to-back optical coherence tomography-ultrasound probe for co-registered three-dimensional intravascular imaging with real-time display

    NASA Astrophysics Data System (ADS)

    Li, Jiawen; Ma, Teng; Jing, Joseph; Zhang, Jun; Patel, Pranav M.; Shung, K. Kirk; Zhou, Qifa; Chen, Zhongping

    2014-03-01

    We have developed a novel integrated optical coherence tomography (OCT)-intravascular ultrasound (IVUS) probe, with a 1.5 mm-long rigid-part and 0.9 mm outer diameter, for real-time intracoronary imaging of atherosclerotic plaques and guiding interventional procedures. By placing the OCT ball lens and IVUS 45MHz single element transducer back-to-back at the same axial position, this probe can provide automatically co-registered, co-axial OCT-IVUS imaging. To demonstrate its capability, 3D OCT-IVUS imaging of a pig's coronary artery in real-time displayed in polar coordinates, as well as images of two major types of advanced plaques in human cadaver coronary segments, was obtained using this probe and our upgraded system. Histology validation is also presented.

  11. Real-time swept source optical coherence tomography imaging of the human airway using a microelectromechanical system endoscope and digital signal processor

    PubMed Central

    Su, Jianping; Zhang, Jun; Yu, Lingfeng; Colt, Henri G; Brenner, Matthew; Chen, Zhongping

    2009-01-01

    A fast-scan-rate swept laser for optical coherence tomography (OCT) is suitable to record and analyze a 3-D image volume. However, the whole OCT system speed is limited by data streaming, processing, and storage. In this case, postprocessing is a common technique. Endoscopic clinical applications prefer onsite diagnosis, which requires a real-time technique. Parallel digital signal processors were applied to stream and process data directly from a data digitizer. A real-time system with 20-kHz axial line speed, which was limited only by our swept laser scan rate, was implemented. To couple with the system speed, an endoscope based on an improved 3-D microelectromechanical motor (diameter 1.5 mm, length 9.4 mm) was developed. In vivo 3-D imaging of the human airway was demonstrated. PMID:18601523

  12. Real-time swept source optical coherence tomography imaging of the human airway using a microelectromechanical system endoscope and digital signal processor.

    PubMed

    Su, Jianping; Zhang, Jun; Yu, Lingfeng; G Colt, Henri; Brenner, Matthew; Chen, Zhongping

    2008-01-01

    A fast-scan-rate swept laser for optical coherence tomography (OCT) is suitable to record and analyze a 3-D image volume. However, the whole OCT system speed is limited by data streaming, processing, and storage. In this case, postprocessing is a common technique. Endoscopic clinical applications prefer onsite diagnosis, which requires a real-time technique. Parallel digital signal processors were applied to stream and process data directly from a data digitizer. A real-time system with 20-kHz axial line speed, which was limited only by our swept laser scan rate, was implemented. To couple with the system speed, an endoscope based on an improved 3-D microelectromechanical motor (diameter 1.5 mm, length 9.4 mm) was developed. In vivo 3-D imaging of the human airway was demonstrated.

  13. Real Time Network Assessment

    DTIC Science & Technology

    2013-07-12

    Demonstrate a simple system Conduct a feasibility assessment of data storage, maintenance, and integration requirements Test a web-based data feed...Real Time Network Assessment Prototype We demonstrated the feasibility of linking near real time network analytics to mashups and web- based...combining similar concepts into single node) Stemmers Thesauri application Network position Statistical common patterns Pronoun identification

  14. Optical Beam Control Using Adaptive Optics

    DTIC Science & Technology

    2005-12-01

    30 1. Principles of Operation......................................................................31 VI. USING ZERNIKE POLYNOMIALS TO...help patience in helping me to understand the underlying principles of optics. xiv THIS PAGE INTENTIONALLY...correct this using adaptive optics. Adaptive Optics first got its start in 215 AD with the destruction of the Roman Fleet by Archimedes (Lamberson

  15. Optical monitor for real time thickness change measurements via lateral-translation induced phase-stepping interferometry

    DOEpatents

    Rushford, Michael C.

    2002-01-01

    An optical monitoring instrument monitors etch depth and etch rate for controlling a wet-etching process. The instrument provides means for viewing through the back side of a thick optic onto a nearly index-matched interface. Optical baffling and the application of a photoresist mask minimize spurious reflections to allow for monitoring with extremely weak signals. A Wollaston prism enables linear translation for phase stepping.

  16. Real-time Molecular Study of Bystander Effects of Low dose Low LET radiation Using Living Cell Imaging and Nanoparticale Optics

    SciTech Connect

    Natarajan, Mohan; Xu, Nancy R; Mohan, Sumathy

    2013-06-03

    cGy on coverslips will be examined for (a) low LET radiation-induced alterations of cellular function and its physiological relevance in real time; and (b) radiation damage triggered bystander effect on the neighboring unirradiated cells. First, to determine the low LET radiation induced alteration of cellular function we will examine: (i) the real time transformation of single membrane transporters in single living cells; (ii) the pump efficiency of membrane efflux pump of live cells in real time at the molecular level; (iii) the kinetics of single-ligand receptor interaction on single live cell surface (Figure C); and (iv) alteration in chromosome replication in living cell. Second, to study the radiation triggered bystander responses, we will examine one of the key signaling pathway i.e. TNF- alpha/NF-kappa B mediated signaling. TNF-alpha specific nano particle sensors (green) will be developed to detect the releasing dynamics, transport mechanisms and ligand-receptor binding on live cell surface in real time. A second sensor (blue) will be developed to simultaneously monitor the track of NF-kB inside the cell. The proposed nano-particle optics approach would complement our DOE funded study on biochemical mechanisms of TNF-alpha- NF-kappa B-mediated bystander effect.

  17. Gait adaptation to visual kinematic perturbations using a real-time closed-loop brain-computer interface to a virtual reality avatar

    NASA Astrophysics Data System (ADS)

    Phat Luu, Trieu; He, Yongtian; Brown, Samuel; Nakagame, Sho; Contreras-Vidal, Jose L.

    2016-06-01

    Objective. The control of human bipedal locomotion is of great interest to the field of lower-body brain-computer interfaces (BCIs) for gait rehabilitation. While the feasibility of closed-loop BCI systems for the control of a lower body exoskeleton has been recently shown, multi-day closed-loop neural decoding of human gait in a BCI virtual reality (BCI-VR) environment has yet to be demonstrated. BCI-VR systems provide valuable alternatives for movement rehabilitation when wearable robots are not desirable due to medical conditions, cost, accessibility, usability, or patient preferences. Approach. In this study, we propose a real-time closed-loop BCI that decodes lower limb joint angles from scalp electroencephalography (EEG) during treadmill walking to control a walking avatar in a virtual environment. Fluctuations in the amplitude of slow cortical potentials of EEG in the delta band (0.1-3 Hz) were used for prediction; thus, the EEG features correspond to time-domain amplitude modulated potentials in the delta band. Virtual kinematic perturbations resulting in asymmetric walking gait patterns of the avatar were also introduced to investigate gait adaptation using the closed-loop BCI-VR system over a period of eight days. Main results. Our results demonstrate the feasibility of using a closed-loop BCI to learn to control a walking avatar under normal and altered visuomotor perturbations, which involved cortical adaptations. The average decoding accuracies (Pearson’s r values) in real-time BCI across all subjects increased from (Hip: 0.18 ± 0.31 Knee: 0.23 ± 0.33 Ankle: 0.14 ± 0.22) on Day 1 to (Hip: 0.40 ± 0.24 Knee: 0.55 ± 0.20 Ankle: 0.29 ± 0.22) on Day 8. Significance. These findings have implications for the development of a real-time closed-loop EEG-based BCI-VR system for gait rehabilitation after stroke and for understanding cortical plasticity induced by a closed-loop BCI-VR system.

  18. Non-conductive and miniature fiber-optic imaging system for real-time detection of neuronal activity in time-varying electromagnetic fields.

    PubMed

    Saito, Atsushi; Takahashi, Masayuki; Jimbo, Yasuhiko; Nakasono, Satoshi

    2017-01-15

    Establishing an appropriate threshold value for neuronal modulation by time-varying electromagnetic field (EMF) exposure is important for developing international guidelines to protect against the potential health effects, and to design a variety of medical devices. However, it is technically difficult to achieve real-time detection of neuronal activity under repetitive and long-term exposure to EMF. For this purpose, we developed a non-conductive, miniature, and flexible fiber-optic imaging system that does not affect the electromagnetic noise, induction heating, or vibration in a high-intensity and repetitive time-varying EMF exposure. Using the proposed system, we succeeded at real-time detection of spontaneous Ca(2+) oscillations in single neuronal and glial cells, as well as synchronized bursting activities of multiple neuronal networks at a micrometer-scale and millisecond-order spatiotemporal resolution during long-term EMF exposure (sinusoidal wave, 20kHz, 8.6mT, >30min). The results indicated that short-term (<5min) exposure-related neuronal modulation was not detectable; however, long-term (15-30min) exposure was observed to depress neuronal activities. In addition, the simultaneous and real-time recording of neuronal activity and the environmental temperature revealed that the neuronal modulation was accompanied by a 0.5-1°C rise in the temperature of the culture medium induced by the heat generation of exposure coils. These findings suggest that our real-time imaging system can be used for precise evaluation of the threshold values and clarification of the mechanisms of neuronal modulation induced by time-varying EMF exposure.

  19. Adaptive Optical Scanning Holography

    PubMed Central

    Tsang, P. W. M.; Poon, Ting-Chung; Liu, J.-P.

    2016-01-01

    Optical Scanning Holography (OSH) is a powerful technique that employs a single-pixel sensor and a row-by-row scanning mechanism to capture the hologram of a wide-view, three-dimensional object. However, the time required to acquire a hologram with OSH is rather lengthy. In this paper, we propose an enhanced framework, which is referred to as Adaptive OSH (AOSH), to shorten the holographic recording process. We have demonstrated that the AOSH method is capable of decreasing the acquisition time by up to an order of magnitude, while preserving the content of the hologram favorably. PMID:26916866

  20. Adaptive Optical Scanning Holography

    NASA Astrophysics Data System (ADS)

    Tsang, P. W. M.; Poon, Ting-Chung; Liu, J.-P.

    2016-02-01

    Optical Scanning Holography (OSH) is a powerful technique that employs a single-pixel sensor and a row-by-row scanning mechanism to capture the hologram of a wide-view, three-dimensional object. However, the time required to acquire a hologram with OSH is rather lengthy. In this paper, we propose an enhanced framework, which is referred to as Adaptive OSH (AOSH), to shorten the holographic recording process. We have demonstrated that the AOSH method is capable of decreasing the acquisition time by up to an order of magnitude, while preserving the content of the hologram favorably.

  1. Real-time optical recording of β1-adrenergic receptor activation reveals supersensitivity of the Arg389 variant to carvedilol

    PubMed Central

    Rochais, Francesca; Vilardaga, Jean-Pierre; Nikolaev, Viacheslav O.; Bünemann, Moritz; Lohse, Martin J.; Engelhardt, Stefan

    2007-01-01

    Antagonists of β-adrenergic receptors (β-ARs) have become a main therapeutic regimen for the treatment of heart failure even though the mechanisms of their beneficial effects are still poorly understood. Here, we used fluorescent resonance energy transfer–based (FRET-based) approaches to directly monitor activation of the β1-AR and downstream signaling. While the commonly used β-AR antagonists metoprolol, bisoprolol, and carvedilol displayed varying degrees of inverse agonism on the Gly389 variant of the receptor (i.e., actively switching off the β1-AR), surprisingly, only carvedilol showed very specific and marked inverse agonist effects on the more frequent Arg389 variant. These specific effects of carvedilol on the Arg389 variant of the β1-AR were also seen for control of beating frequency in rat cardiac myocytes expressing the 2 receptor variants. This FRET sensor permitted direct observation of activation of the β1-AR in living cells in real time. It revealed that β1-AR variants dramatically differ in their responses to diverse beta blockers, with possible consequences for their clinical use. PMID:17200720

  2. Real-time optical monitoring of thin film growth by in situ pyrometry through multiple layers and effective media approximation modeling

    SciTech Connect

    Benedic, F.; Bruno, P.; Pigeat, Ph.

    2007-03-26

    A model combining multiple layer description and effective media approximation is developed for pyrometry in the case of thin film synthesis, in order to estimate the film property evolution along its thickness during the growth process in real time. The model is used to investigate optical properties of polycrystalline diamond film prepared by H{sub 2}/CH{sub 4}/N{sub 2} microwave plasma. It is shown that in the presence of nitrogen, the growth is strongly nonhomogeneous. The deposit, initially composed of large amounts of void and nondiamond phases, evolves rapidly towards highest quality dense film where the diamond phase is predominant.

  3. Diffuse optical tomography with an amplified ultrafast laser and a single-shot streak camera: application to real-time in vivo songbird neuro-imaging

    NASA Astrophysics Data System (ADS)

    Guillet de Chatellus, Hugues; Vignal, Clémentine; Ramstein, Stéphane; Verjat, Nicolas; Mathevon, Nicolas; Mottin, Stéphane

    2005-10-01

    A new ultrafast Diffuse Optical Tomography (DOT) has been developed for real time in vivo brain metabolism monitoring in songbird. The technique is based on space resolved time of flight measurements of the photons across the brain tissues. A three dimensional reconstruction of the brain activity is foreseeable by means of a double space and time sampling of the reflectance signal. The setup and the treatment procedure are described in depth and promising preliminary results showing the response of brain tissues to hypercapnia stimulations (increase of CO2) are presented.

  4. Real-time simultaneous single snapshot of optical properties and blood flow using coherent spatial frequency domain imaging (cSFDI)

    PubMed Central

    Ghijsen, Michael; Choi, Bernard; Durkin, Anthony J.; Gioux, Sylvain; Tromberg, Bruce J.

    2016-01-01

    In this work we present and validate a wide-field method for the real-time mapping of tissue absorption, scattering and blood flow properties over wide regions of tissue (15 cm x 15 cm) with high temporal resolution (50 frames per second). We achieve this by applying Fourier Domain demodulation techniques to coherent spatial frequency domain imaging to extract optical properties and speckle flow index from a single snapshot. Applying this technique to forearm reactive hyperemia protocols demonstrates the ability to resolve intrinsic physiological signals such as the heart beat waveform and the buildup of deoxyhemoglobin associated with oxygen consumption. PMID:27231595

  5. Real-Time Analysis of Isoprene in Breath by Using Ultraviolet-Absorption Spectroscopy with a Hollow Optical Fiber Gas Cell.

    PubMed

    Iwata, Takuro; Katagiri, Takashi; Matsuura, Yuji

    2016-12-05

    A breath analysis system based on ultraviolet-absorption spectroscopy was developed by using a hollow optical fiber as a gas cell for real-time monitoring of isoprene in breath. The hollow optical fiber functions as an ultra-small-volume gas cell with a long path. The measurement sensitivity of the system was evaluated by using nitric-oxide gas as a gas sample. The evaluation result showed that the developed system, using a laser-driven, high-intensity light source and a 3-m-long, aluminum-coated hollow optical fiber, could successfully measure nitric-oxide gas with a 50 ppb concentration. An absorption spectrum of a breath sample in the wavelength region of around 200-300 nm was measured, and the measured spectrum revealed the main absorbing components in breath as water vapor, isoprene, and ozone converted from oxygen by radiation of ultraviolet light. The concentration of isoprene in breath was estimated by multiple linear regression. The regression analysis results showed that the proposed analysis system enables real-time monitoring of isoprene during the exhaling of breath. Accordingly, it is suitable for measuring the circadian variation of isoprene.

  6. Real-Time Analysis of Isoprene in Breath by Using Ultraviolet-Absorption Spectroscopy with a Hollow Optical Fiber Gas Cell

    PubMed Central

    Iwata, Takuro; Katagiri, Takashi; Matsuura, Yuji

    2016-01-01

    A breath analysis system based on ultraviolet-absorption spectroscopy was developed by using a hollow optical fiber as a gas cell for real-time monitoring of isoprene in breath. The hollow optical fiber functions as an ultra-small-volume gas cell with a long path. The measurement sensitivity of the system was evaluated by using nitric-oxide gas as a gas sample. The evaluation result showed that the developed system, using a laser-driven, high-intensity light source and a 3-m-long, aluminum-coated hollow optical fiber, could successfully measure nitric-oxide gas with a 50 ppb concentration. An absorption spectrum of a breath sample in the wavelength region of around 200–300 nm was measured, and the measured spectrum revealed the main absorbing components in breath as water vapor, isoprene, and ozone converted from oxygen by radiation of ultraviolet light. The concentration of isoprene in breath was estimated by multiple linear regression. The regression analysis results showed that the proposed analysis system enables real-time monitoring of isoprene during the exhaling of breath. Accordingly, it is suitable for measuring the circadian variation of isoprene. PMID:27929387

  7. New Adaptive Optics Technique Demonstrated

    NASA Astrophysics Data System (ADS)

    2007-03-01

    First ever Multi-Conjugate Adaptive Optics at the VLT Achieves First Light On the evening of 25 March 2007, the Multi-Conjugate Adaptive Optics Demonstrator (MAD) achieved First Light at the Visitor Focus of Melipal, the third Unit Telescope of the Very Large Telescope (VLT). MAD allowed the scientists to obtain images corrected for the blurring effect of atmospheric turbulence over the full 2x2 arcminute field of view. This world premiere shows the promises of a crucial technology for Extremely Large Telescopes. ESO PR Photo 19a/07 ESO PR Photo 19a/07 The MCAO Demonstrator Telescopes on the ground suffer from the blurring effect induced by atmospheric turbulence. This turbulence causes the stars to twinkle in a way which delights the poets but frustrates the astronomers, since it blurs the fine details of the images. However, with Adaptive Optics (AO) techniques, this major drawback can be overcome so that the telescope produces images that are as sharp as theoretically possible, i.e., approaching space conditions. Adaptive Optics systems work by means of a computer-controlled deformable mirror (DM) that counteracts the image distortion induced by atmospheric turbulence. It is based on real-time optical corrections computed from image data obtained by a 'wavefront sensor' (a special camera) at very high speed, many hundreds of times each second. The concept is not new. Already in 1989, the first Adaptive Optics system ever built for Astronomy (aptly named "COME-ON") was installed on the 3.6-m telescope at the ESO La Silla Observatory, as the early fruit of a highly successful continuing collaboration between ESO and French research institutes (ONERA and Observatoire de Paris). Ten years ago, ESO initiated an Adaptive Optics program to serve the needs for its frontline VLT project. Today, the Paranal Observatory is without any doubt one of the most advanced of its kind with respect to AO with no less than 7 systems currently installed (NACO, SINFONI, CRIRES and

  8. A low-cost, portable optical sensing system with wireless communication compatible of real-time and remote detection of dissolved ammonia

    NASA Astrophysics Data System (ADS)

    Deng, Shijie; Doherty, William; McAuliffe, Michael AP; Salaj-Kosla, Urszula; Lewis, Liam; Huyet, Guillaume

    2016-06-01

    A low-cost and portable optical chemical sensor based ammonia sensing system that is capable of detecting dissolved ammonia up to 5 ppm is presented. In the system, an optical chemical sensor is designed and fabricated for sensing dissolved ammonia concentrations. The sensor uses eosin as the fluorescence dye which is immobilized on the glass substrate by a gas-permeable protection layer. A compact module is developed to hold the optical components, and a battery powered micro-controller system is designed to read out and process the data measured. The system operates without the requirement of laboratory instruments that makes it cost effective and highly portable. Moreover, the calculated results in the system can be transmitted to a PC wirelessly, which allows the remote and real-time monitoring of dissolved ammonia.

  9. Adaptive optics with pupil tracking for high resolution retinal imaging.

    PubMed

    Sahin, Betul; Lamory, Barbara; Levecq, Xavier; Harms, Fabrice; Dainty, Chris

    2012-02-01

    Adaptive optics, when integrated into retinal imaging systems, compensates for rapidly changing ocular aberrations in real time and results in improved high resolution images that reveal the photoreceptor mosaic. Imaging the retina at high resolution has numerous potential medical applications, and yet for the development of commercial products that can be used in the clinic, the complexity and high cost of the present research systems have to be addressed. We present a new method to control the deformable mirror in real time based on pupil tracking measurements which uses the default camera for the alignment of the eye in the retinal imaging system and requires no extra cost or hardware. We also present the first experiments done with a compact adaptive optics flood illumination fundus camera where it was possible to compensate for the higher order aberrations of a moving model eye and in vivo in real time based on pupil tracking measurements, without the real time contribution of a wavefront sensor. As an outcome of this research, we showed that pupil tracking can be effectively used as a low cost and practical adaptive optics tool for high resolution retinal imaging because eye movements constitute an important part of the ocular wavefront dynamics.

  10. Adaptive optics with pupil tracking for high resolution retinal imaging

    PubMed Central

    Sahin, Betul; Lamory, Barbara; Levecq, Xavier; Harms, Fabrice; Dainty, Chris

    2012-01-01

    Adaptive optics, when integrated into retinal imaging systems, compensates for rapidly changing ocular aberrations in real time and results in improved high resolution images that reveal the photoreceptor mosaic. Imaging the retina at high resolution has numerous potential medical applications, and yet for the development of commercial products that can be used in the clinic, the complexity and high cost of the present research systems have to be addressed. We present a new method to control the deformable mirror in real time based on pupil tracking measurements which uses the default camera for the alignment of the eye in the retinal imaging system and requires no extra cost or hardware. We also present the first experiments done with a compact adaptive optics flood illumination fundus camera where it was possible to compensate for the higher order aberrations of a moving model eye and in vivo in real time based on pupil tracking measurements, without the real time contribution of a wavefront sensor. As an outcome of this research, we showed that pupil tracking can be effectively used as a low cost and practical adaptive optics tool for high resolution retinal imaging because eye movements constitute an important part of the ocular wavefront dynamics. PMID:22312577

  11. Real-time dynamic optical imaging of ACC-M tumor cells killed by HSV-tk/ACV system.

    PubMed

    Xiong, Tao; Li, Yongjin; Li, Zhiyang; Xie, Xiangmo; Lu, Lisha

    2013-01-01

    HSV-tk/ACV induced and killed human adenoid cystic carcinoma cell (ACC-M) in vivo and in vitro, which were observed through optical imaging and green fluorescence protein (GFP) tagging technique. ACC-M was transfected with TK-GFP, and the single clone cell ACC-M-TK-GFP was selected by G418. With fluorescent stereomicroscope, whole-body fluorescent imaging system and fluorescent microscope, we could observe ACV treated ACC-M-TK-GFP cells in cell level and nude mice. The therapies of tumor were visualized clearly with optical imaging. This study proves that optical imaging is a very good approach for studying the effect of HSV-tk/ACV on the ACC-M tumor cells and decreasing the amount of vessel about tumors cell. Optical imaging will become a visual groundwork for monitoring tumor growth and evaluating in vivo curative effect of antitumor drugs.

  12. Liquid lens enabling real-time focus and tilt compensation for optical image stabilization in camera modules

    NASA Astrophysics Data System (ADS)

    Simon, Eric; Craen, Pierre; Gaton, Hilario; Jacques-Sermet, Olivier; Laune, Frédéric; Legrand, Julien; Maillard, Mathieu; Tallaron, Nicolas; Verplanck, Nicolas; Berge, Bruno

    2010-05-01

    A new generation of liquid lenses based on electrowetting has been developed, using a multi-electrode design, enabling to induce optical tilt and focus corrections in the same component. The basic principle is to rely on a conical shape for supporting the liquid interface, the conical shape insuring a restoring force for the liquid liquid interface to come at the center position. The multi-electrode design enables to induce an average tilt of the liquid liquid interface when a bias voltage is applied to the different electrodes. This tilt is reversible, vanishing when voltage bias is cancelled. Possible application of this new lens component is the realization of miniature camera featuring auto-focus and optical image stabilization (OIS) without any mobile mechanical part. Experimental measurements of actual performances of liquid lens component will be presented : focus and tilt amplitude, residual optical wave front error and response time.

  13. Adaptive optics program at TMT

    NASA Astrophysics Data System (ADS)

    Boyer, C.; Adkins, Sean; Andersen, David R.; Atwood, Jenny; Bo, Yong; Byrnes, Peter; Caputa, Kris; Cavaco, Jeff; Ellerbroek, Brent; Gilles, Luc; Gregory, James; Herriot, Glen; Hickson, Paul; Ljusic, Zoran; Manter, Darren; Marois, Christian; Otárola, Angel; Pagès, Hubert; Schoeck, Matthias; Sinquin, Jean-Christophe; Smith, Malcolm; Spano, Paolo; Szeto, Kei; Tang, Jinlong; Travouillon, Tony; Véran, Jean-Pierre; Wang, Lianqi; Wei, Kai

    2014-07-01

    The TMT first light Adaptive Optics (AO) facility consists of the Narrow Field Infra-Red AO System (NFIRAOS) and the associated Laser Guide Star Facility (LGSF). NFIRAOS is a 60 × 60 laser guide star (LGS) multi-conjugate AO (MCAO) system, which provides uniform, diffraction-limited performance in the J, H, and K bands over 17-30 arc sec diameter fields with 50 per cent sky coverage at the galactic pole, as required to support the TMT science cases. NFIRAOS includes two deformable mirrors, six laser guide star wavefront sensors, and three low-order, infrared, natural guide star wavefront sensors within each client instrument. The first light LGSF system includes six sodium lasers required to generate the NFIRAOS laser guide stars. In this paper, we will provide an update on the progress in designing, modeling and validating the TMT first light AO systems and their components over the last two years. This will include pre-final design and prototyping activities for NFIRAOS, preliminary design and prototyping activities for the LGSF, design and prototyping for the deformable mirrors, fabrication and tests for the visible detectors, benchmarking and comparison of different algorithms and processing architecture for the Real Time Controller (RTC) and development and tests of prototype candidate lasers. Comprehensive and detailed AO modeling is continuing to support the design and development of the first light AO facility. Main modeling topics studied during the last two years include further studies in the area of wavefront error budget, sky coverage, high precision astrometry for the galactic center and other observations, high contrast imaging with NFIRAOS and its first light instruments, Point Spread Function (PSF) reconstruction for LGS MCAO, LGS photon return and sophisticated low order mode temporal filtering.

  14. Optical chaos and hybrid WDM/TDM based large capacity quasi-distributed sensing network with real-time fiber fault monitoring.

    PubMed

    Luo, Yiyang; Xia, Li; Xu, Zhilin; Yu, Can; Sun, Qizhen; Li, Wei; Huang, Di; Liu, Deming

    2015-02-09

    An optical chaos and hybrid wavelength division multiplexing/time division multiplexing (WDM/TDM) based large capacity quasi-distributed sensing network with real-time fiber fault monitoring is proposed. Chirped fiber Bragg grating (CFBG) intensity demodulation is adopted to improve the dynamic range of the measurements. Compared with the traditional sensing interrogation methods in time, radio frequency and optical wavelength domains, the measurand sensing and the precise locating of the proposed sensing network can be simultaneously interrogated by the relative amplitude change (RAC) and the time delay of the correlation peak in the cross-correlation spectrum. Assisted with the WDM/TDM technology, hundreds of sensing units could be potentially multiplexed in the multiple sensing fiber lines. Based on the proof-of-concept experiment for axial strain measurement with three sensing fiber lines, the strain sensitivity up to 0.14% RAC/με and the precise locating of the sensors are achieved. Significantly, real-time fiber fault monitoring in the three sensing fiber lines is also implemented with a spatial resolution of 2.8 cm.

  15. Linking aerosol size and optical properties to trace gases emitted from biomass burning in real-time

    NASA Astrophysics Data System (ADS)

    McMeeking, G. R.; Carrico, C. M.; Stockwell, C.; Yokelson, R. J.; Veres, P. R.; DeMott, P. J.; Kreidenweis, S. M.

    2014-12-01

    Biomass burning aerosols have large impacts on regional and global climate that are partly determined by their optical properties. The optical properties of aerosol depend on their size and composition, which in turn are related to fire combustion processes. Here we investigate relationships between a large suite of trace gases and aerosol size and optical properties to better understand processes governing the optical properties of fresh biomass burning aerosol emissions. We examined over 100 individual burns of biomass fuels during the Fire Laboratory at Missoula Experiment 4 (FLAME 4). Emissions were measured directly from an exhaust stack designed to capture all emissions from relatively small-scale fires burned at the base of a large burn chamber. Trace gas species were measured using a combination of an open-path Fourier transform infrared spectrometer (OP-FTIR) and proton-transfer mass spectrometer (PTR-MS). Aerosol optical properties at 870 nm were measured using a photoacoustic extinctiometer (PAX) and particle size distributions were measured using a Fast Mobility Particle Sizer (FMPS) and Aerodynamic Particle Sizer. The rapid response of the instruments allowed for comparisons of the emissions and particle properties over the duration of the fire. For example, we observed correlations between aerosol absorption, particle size, and gas-phase species associated with different types of combustion such as flaming and smoldering. We also report fire-integrated emissions for aerosol absorption and scattering coefficients and compare these to other fire-integrated properties. Many of our burn experiments examined a number of fuels that had not before been characterized in laboratory conditions, including a number of peat fuels, African savanna grasses and crop residuals.

  16. Real Time Optics of the Growth of Textured Silicon Films in Photovoltaics: Final Technical Report, 1 August 1999--12 August 2002

    SciTech Connect

    Collins, R. W.; Wronski, C. R.

    2003-10-01

    Novel optical instruments, including single and dual rotating-compensator multichannel ellipsometers, have been designed and developed to probe the evolution of the microstructure, spectroscopic optical properties, and other materials characteristics during the fabrication and processing of individual thin films and thin-film structures used in photovoltaic devices. These instruments provide a foundation for next-generation process design/control and metrology in existing and future photovoltaics technologies. In this project, the materials system studied in the greatest detail was thin-film silicon, fabricated at low temperatures by plasma-enhanced chemical vapor deposition. Real-time measurements of such thin films by multichannel ellipsometry have established deposition phase diagrams that provide guiding principles for multistep fabrication of high-performance amorphous (a-Si:H) and microcrystalline (mc-Si:H) solar cells. Such phase diagrams have also served to disprove conventional wisdom in the fabrication of thin-film solar cell structures, thus avoiding future unproductive research efforts.

  17. Real Time Data Analysis Techniques

    NASA Astrophysics Data System (ADS)

    Silberberg, George G.

    1983-03-01

    By the early 1970s, classical photo-optical range instrumentation technology (as a means of gathering weapons' system performance data) had become a costly and inefficient process. Film costs were increasing due to soaring silver prices. Time required to process, read, and produce optical data was becoming unacceptable as a means of supporting weapon system development programs. NWC investigated the feasibility of utilizing Closed Circuit Television (CCTV) technology as an alternative solution for providing optical data. In 1978 a program entitled Metric Video (measurements from video images) was formulated at the Naval Weapons Center, China Lake, California. The purpose of this program was to provide timely data, to reduce the number of operating personnel, and to lower data acquisition costs. Some of the task elements for this program included a near real-time vector miss-distance system, a weapons scoring system, a velocity measuring system, a time-space position system, and a system to replace film cameras for gathering real-time engineering sequential data. These task elements and the development of special hardware and techniques to achieve real-time data will be discussed briefly in this paper.

  18. FPGA implementation of Santos-Victor optical flow algorithm for real-time image processing: an useful attempt

    NASA Astrophysics Data System (ADS)

    Cobos Arribas, Pedro; Monasterio Huelin Macia, Felix

    2003-04-01

    A FPGA based hardware implementation of the Santos-Victor optical flow algorithm, useful in robot guidance applications, is described in this paper. The system used to do contains an ALTERA FPGA (20K100), an interface with a digital camera, three VRAM memories to contain the data input and some output memories (a VRAM and a EDO) to contain the results. The system have been used previously to develop and test other vision algorithms, such as image compression, optical flow calculation with differential and correlation methods. The designed system let connect the digital camera, or the FPGA output (results of algorithms) to a PC, throw its Firewire or USB port. The problems take place in this occasion have motivated to adopt another hardware structure for certain vision algorithms with special requirements, that need a very hard code intensive processing.

  19. Real-time optical correlator using computer-generated holographic filter on a liquid crystal light valve

    NASA Technical Reports Server (NTRS)

    Chao, Tien-Hsin; Yu, Jeffrey

    1990-01-01

    Limitations associated with the binary phase-only filter often used in optical correlators are presently circumvented in the writing of complex-valued data on a gray-scale spatial light modulator through the use of a computer-generated hologram (CGH) algorithm. The CGH encodes complex-valued data into nonnegative real CGH data in such a way that it may be encoded in any of the available gray-scale spatial light modulators. A CdS liquid-crystal light valve is used for the complex-valued CGH encoding; computer simulations and experimental results are compared, and the use of such a CGH filter as the synapse hologram in a holographic optical neural net is discussed.

  20. A Novel Hand-Held Optical Imager with Real-Time Co-Registration Facilities Towards Diagnostic Mammography

    DTIC Science & Technology

    2012-01-01

    underneath the breast), as shown in Fig. 9. No fluorescenc was injected into the human subject. Average optical properties of normal breast tissues (i.e...right (i.e., toward the 3 o’ clock direc - tion from the center). The images show that within this central region, there is maximum signal intensity...perfect and imperfect (100:1) uptake conditions in ( liquid ) tissue phantoms and in vitro. Upon summation of multiple scans (fluorescence intensity images

  1. A Novel Hand-Held Optical Imager with Real-Time Co-registration Facilities toward Diagnostic Mammography

    DTIC Science & Technology

    2010-01-01

    used to simulate a tumor and was placed underneath the flap of the breast tissue (i.e. between breast tissue and chest wall, underneath the tissue...optical imaging. Work Completed to Date: Proposed Task A: Implement a 3D motion tracking device in order to randomly track the movement of the...hand-held probe on the tissue surface. A 3D tracking system was implemented on the probe in order to perform coregistered imaging using MATLAB /LabView

  2. Real-time radiography

    SciTech Connect

    Bossi, R.H.; Oien, C.T.

    1981-02-26

    Real-time radiography is used for imaging both dynamic events and static objects. Fluorescent screens play an important role in converting radiation to light, which is then observed directly or intensified and detected. The radiographic parameters for real-time radiography are similar to conventional film radiography with special emphasis on statistics and magnification. Direct-viewing fluoroscopy uses the human eye as a detector of fluorescent screen light or the light from an intensifier. Remote-viewing systems replace the human observer with a television camera. The remote-viewing systems have many advantages over the direct-viewing conditions such as safety, image enhancement, and the capability to produce permanent records. This report reviews real-time imaging system parameters and components.

  3. Real-time association rate constant measurement using combination tapered fiber-optic biosensor (CTFOB) dip-probes

    NASA Astrophysics Data System (ADS)

    Simmonds, Boris; Wang, Chun-Wei; Kapoor, Rakesh

    2010-02-01

    This document reports a novel method of measuring association rate constant (ka) for antibody-antigen interaction using evanescent wave-based combination tapered fiber-optic biosensor (CTFOB) dip-probes. The method was demonstrated by measuring association rate constant for bovine serum albumin (BSA) and anti-BSA antibody interaction. "Direct method" was used for detection; goat anti-BSA "capture" antibodies were immobilized on the probe surfaces while the antigen (BSA) was directly labeled with Alexa 488 dye. The probes were subsequently submerged in 3nM Labeled BSA in egg albumin (1 mg/ml). The fluorescence signal recorded was proportional to BSA anti-BSA conjugates and continuous signal was acquired suing a fiber optic spectrometer (Ocean Optics, Inc.). A 476 nm diode laser was use as an excitation source. Association constant was estimated from a plot of signal as a function of time. Measured association rate constant ka for the binding of BSA with anti-BSA at room temperature is (8.33 +/- 0.01) x 104 M-1s-1.

  4. Single particle optical extinction and scattering allows real time quantitative characterization of drug payload and degradation of polymeric nanoparticles

    NASA Astrophysics Data System (ADS)

    Potenza, M. A. C.; Sanvito, T.; Argentiere, S.; Cella, C.; Paroli, B.; Lenardi, C.; Milani, P.

    2015-12-01

    The behavior of nanoparticles in biological systems is determined by their dimensions, size distribution, shape, surface chemistry, density, drug loading and stability; the characterization of these parameters in realistic conditions and the possibility to follow their evolution in vitro and in vivo are, in most of the cases, far from the capabilities of the standard characterization technologies. Optical techniques such as dynamic light scattering (DLS) are, in principle, well suited for in line characterization of nanoparticle, however their fail in characterizing the evolution of nanoparticle in solution where change in particle dimension and density is present. Here we present an in-line optical technique based on single particle extinction and scattering (SPES) overcoming the limitations typical of DLS and allowing for the efficient characterization of nanoparticle polydispersity, index of refraction and degradation dynamics in solution. Using SPES, we characterized the evolution of PLGA nanoparticles with different structures and drug payloads in solution and we compared the results with DLS. Our results suggest that SPES could be used as a process analytical technology for pharmaceutical nanoparticle production.

  5. Simultaneous fingerprint and high-wavenumber fiber-optic Raman spectroscopy enhances real-time in vivo diagnosis of adenomatous polyps during colonoscopy.

    PubMed

    Bergholt, Mads Sylvest; Lin, Kan; Wang, Jianfeng; Zheng, Wei; Xu, Hongzhi; Huang, Qingwen; Ren, Jian-Lin; Ho, Khek Yu; Teh, Ming; Srivastava, Supriya; Wong, Benjamin; Yeoh, Khay Guan; Huang, Zhiwei

    2016-04-01

    Colorectal cancer can be prevented if detected early (e.g., precancerous polyps-adenoma). Endoscopic differential diagnosis of hyperplastic polyps (that have little or no risk of malignant transformation) and adenomas (that have prominent malignant latency) remains an unambiguous clinical challenge. Raman spectroscopy is an optical vibrational technique capable of probing biomolecular changes of tissue associated with neoplastic transformation. This work aims to apply a fiber-optic simultaneous fingerprint (FP) and high wavenumber (HW) Raman spectroscopy technique for real-time in vivo assessment of adenomatous polyps during clinical colonoscopy. We have developed a fiber-optic Raman endoscopic technique capable of simultaneously acquiring both the FP (i.e., 800-1800 cm(-1)) and HW (i.e., 2800-3600 cm(-1)) Raman spectra from colorectal tissue subsurface (<200 µm) for real-time assessment of colorectal carcinogenesis. In vivo FP/HW Raman spectra were acquired from 50 patients with 17 colorectal polyps during clinical colonoscopy. Prominent Raman spectral differences (p < 0.001) were found between hyperplastic (n = 118 spectra), adenoma (n = 184 spectra) that could be attributed to changes in inter- and intra-cellular proteins, lipids, DNA and water structures and conformations. Simultaneous FP/HW Raman endoscopy provides a diagnostic sensitivity of 90.9% and specificity of 83.3% for differentiating adenoma from hyperplastic polyps, which is superior to either the FP or HW Raman technique alone. This study shows that simultaneous FP/HW Raman spectroscopy technique has the potential to be a clinically powerful tool for improving early diagnosis of adenomatous polyps in vivo during colonoscopic examination.

  6. Evaluation of artifact reduction in optical coherence tomography angiography with real-time tracking and motion correction technology.

    PubMed

    Camino, Acner; Zhang, Miao; Gao, Simon S; Hwang, Thomas S; Sharma, Utkarsh; Wilson, David J; Huang, David; Jia, Yali

    2016-10-01

    Artifacts introduced by eye motion in optical coherence tomography angiography (OCTA) affect the interpretation of images and the quantification of parameters with clinical value. Eradication of such artifacts in OCTA remains a technical challenge. We developed an algorithm that recognizes five different types of motion artifacts and used it to evaluate the performance of three motion removal technologies. On en face maximum projection of flow images, the summed flow signal in each row and column and the correlation between neighboring rows and columns were calculated. Bright line artifacts were recognized by large summed flow signal. Drifts, distorted lines, and stretch artifacts exhibited abnormal correlation values. Residual lines were simultaneously a local maximum of summed flow and a local minimum of correlation. Tracking-assisted scanning integrated with motion correction technology (MCT) demonstrated higher performance than tracking or MCT alone in healthy and diabetic eyes.

  7. Evaluation of artifact reduction in optical coherence tomography angiography with real-time tracking and motion correction technology

    PubMed Central

    Camino, Acner; Zhang, Miao; Gao, Simon S.; Hwang, Thomas S.; Sharma, Utkarsh; Wilson, David J.; Huang, David; Jia, Yali

    2016-01-01

    Artifacts introduced by eye motion in optical coherence tomography angiography (OCTA) affect the interpretation of images and the quantification of parameters with clinical value. Eradication of such artifacts in OCTA remains a technical challenge. We developed an algorithm that recognizes five different types of motion artifacts and used it to evaluate the performance of three motion removal technologies. On en face maximum projection of flow images, the summed flow signal in each row and column and the correlation between neighboring rows and columns were calculated. Bright line artifacts were recognized by large summed flow signal. Drifts, distorted lines, and stretch artifacts exhibited abnormal correlation values. Residual lines were simultaneously a local maximum of summed flow and a local minimum of correlation. Tracking-assisted scanning integrated with motion correction technology (MCT) demonstrated higher performance than tracking or MCT alone in healthy and diabetic eyes. PMID:27867702

  8. Pixying Behavior: A Versatile Real-Time and Post Hoc Automated Optical Tracking Method for Freely Moving and Head Fixed Animals

    PubMed Central

    Oraby, Hatem; Dominiak, Sina

    2017-01-01

    Abstract Here, we describe an automated optical method for tracking animal behavior in both head-fixed and freely moving animals, in real time and offline. It takes advantage of an off-the-shelf camera system, the Pixy camera, designed as a fast vision sensor for robotics that uses a color-based filtering algorithm at 50 Hz to track objects. Using customized software, we demonstrate the versatility of our approach by first tracking the rostro-caudal motion of individual adjacent row (D1, D2) or arc whiskers (β, γ), or a single whisker and points on the whisker pad, in head-fixed mice performing a tactile task. Next, we acquired high-speed video and Pixy data simultaneously and applied the pixy-based real-time tracking to high-speed video data. With this approach, we expand the temporal resolution of the Pixy camera and track motion (post hoc) at the limit of high-speed video frame rates. Finally, we show that this system is flexible: it can be used to track individual whisker or limb position without any sophisticated object tracking algorithm, it can be used in many lighting conditions including infrared (IR); it can be used to track head rotation and location of multiple animals simultaneously. Our system makes behavioral monitoring possible in virtually any biological setting. PMID:28275712

  9. Structure formation in perfluoropentacene:diindenoperylene blends and its impact on transient effects in the optical properties studied in real-time during growth

    SciTech Connect

    Broch, K.; Gerlach, A. Lorch, C.; Dieterle, J.; Novák, J.; Schreiber, F.; Hinderhofer, A.

    2013-11-07

    We discuss the result of the competing effects of favourable intermolecular interactions and steric incompatibilities due to the size mismatch of perfluoropentacene (PFP) and diindenoperylene (DIP) on the structure formation and associated optical properties in mixed films. Using real-time grazing incidence X-ray diffraction we investigate the size of coherently scattering islands l{sub s} as a function of film thickness and mixing ratio. We find that for PFP:DIP 1:2 blends l{sub s} is by a factor of ∼4 smaller than in pure DIP films, while l{sub s} of the PFP:DIP 2:1 blends is not significantly reduced compared with pure PFP. Yet, we observe an increase in l{sub s} with film thickness for all of the samples, independent on the mixing ratio. In parallel with the structural characterization we investigate the evolution of the absorption spectra in the visible spectral range and its dependence on l{sub s} in situ during film growth using differential reflectance spectroscopy. We observe a surprisingly strong effect of changes in the structural order on the shape of ε{sub 2,} {sub xy}(E), evident by a pronounced evolution of characteristic peaks in the thickness range from 1.6 nm to 9.6 nm. The combined results of the real-time experiments allow to identify the thickness dependent crystal grain size as the origin of the observed transient effects in the absorption spectra.

  10. Structure formation in perfluoropentacene:diindenoperylene blends and its impact on transient effects in the optical properties studied in real-time during growth.

    PubMed

    Broch, K; Gerlach, A; Lorch, C; Dieterle, J; Novák, J; Hinderhofer, A; Schreiber, F

    2013-11-07

    We discuss the result of the competing effects of favourable intermolecular interactions and steric incompatibilities due to the size mismatch of perfluoropentacene (PFP) and diindenoperylene (DIP) on the structure formation and associated optical properties in mixed films. Using real-time grazing incidence X-ray diffraction we investigate the size of coherently scattering islands l(s) as a function of film thickness and mixing ratio. We find that for PFP:DIP 1:2 blends l(s) is by a factor of ~4 smaller than in pure DIP films, while l(s) of the PFP:DIP 2:1 blends is not significantly reduced compared with pure PFP. Yet, we observe an increase in l(s) with film thickness for all of the samples, independent on the mixing ratio. In parallel with the structural characterization we investigate the evolution of the absorption spectra in the visible spectral range and its dependence on l(s) in situ during film growth using differential reflectance spectroscopy. We observe a surprisingly strong effect of changes in the structural order on the shape of ε(2, xy)(E), evident by a pronounced evolution of characteristic peaks in the thickness range from 1.6 nm to 9.6 nm. The combined results of the real-time experiments allow to identify the thickness dependent crystal grain size as the origin of the observed transient effects in the absorption spectra.

  11. A real-time ARMS PCR/high-resolution melt curve assay for the detection of the three primary mitochondrial mutations in Leber’s hereditary optic neuropathy

    PubMed Central

    Ryan, Fergus; O’Dwyer, Veronica; Neylan, Derek

    2016-01-01

    Purpose Approximately 95% of patients who are diagnosed with Leber’s hereditary optic neuropathy (LHON) have one of three mitochondrial point mutations responsible for the disease, G3460A, G11778A, and T14484C. The purpose of this study was to develop a novel multiplex real-time amplification-refractory mutation system (ARMS) PCR combined with high-resolution melt curves to identify the individual mutations involved. The study aimed to provide a more robust, cost- and time-effective mutation detection strategy than that offered with currently available methods. The assay reported in this study will allow diagnostic laboratories to avoid costly next-generation sequencing (NGS) assays for most patients with LHON and to focus resources on patients with unknown mutations that require further analysis. Methods The test uses a combination of multiplex allele-specific PCR (ARMS PCR) in combination with a high-resolution melt curve analysis to detect the presence of the mutations in G3460A, G11778A, and T14484C. PCR primer sets were designed to produce a control PCR product and PCR products only in the presence of the mutations in 3460A, 11778A, and 14484C in a multiplex single tube format. Products produce discrete well-separated melt curves to clearly detect the mutations. Results This novel real-time ARMS PCR/high-resolution melt curve assay accurately detected 95% of the mutations that cause LHON. The test has proved to be robust, cost- and time-effective with the real-time closed tube system taking approximately 1 h to complete. Conclusions A novel real-time ARMS PCR/high-resolution melt curve assay is described for the detection of the three primary mitochondrial mutations in LHON. This test provides a simple, robust, easy-to-read output that is cost- and time-effective, thus providing an alternative method to individual endpoint PCR-restriction fragment length polymorphism (RFLP), PCR followed by Sanger sequencing or pyrosequencing, and next-generation sequencing

  12. Optimal Throughput and Self-adaptability of Robust Real-Time IEEE 802.15.4 MAC for AMI Mesh Network

    NASA Astrophysics Data System (ADS)

    Shabani, Hikma; Mohamud Ahmed, Musse; Khan, Sheroz; Hameed, Shahab Ahmed; Hadi Habaebi, Mohamed

    2013-12-01

    A smart grid refers to a modernization of the electricity system that brings intelligence, reliability, efficiency and optimality to the power grid. To provide an automated and widely distributed energy delivery, the smart grid will be branded by a two-way flow of electricity and information system between energy suppliers and their customers. Thus, the smart grid is a power grid that integrates data communication networks which provide the collected and analysed data at all levels in real time. Therefore, the performance of communication systems is so vital for the success of smart grid. Merit to the ZigBee/IEEE802.15.4std low cost, low power, low data rate, short range, simplicity and free licensed spectrum that makes wireless sensor networks (WSNs) the most suitable wireless technology for smart grid applications. Unfortunately, almost all ZigBee channels overlap with wireless local area network (WLAN) channels, resulting in severe performance degradation due to interference. In order to improve the performance of communication systems, this paper proposes an optimal throughput and self-adaptability of ZigBee/IEEE802.15.4std for smart grid.

  13. Adaptive-optics optical coherence tomography processing using a graphics processing unit.

    PubMed

    Shafer, Brandon A; Kriske, Jeffery E; Kocaoglu, Omer P; Turner, Timothy L; Liu, Zhuolin; Lee, John Jaehwan; Miller, Donald T

    2014-01-01

    Graphics processing units are increasingly being used for scientific computing for their powerful parallel processing abilities, and moderate price compared to super computers and computing grids. In this paper we have used a general purpose graphics processing unit to process adaptive-optics optical coherence tomography (AOOCT) images in real time. Increasing the processing speed of AOOCT is an essential step in moving the super high resolution technology closer to clinical viability.

  14. Optical Coherence Tomography for Retinal Surgery: Perioperative Analysis to Real-Time Four-Dimensional Image-Guided Surgery

    PubMed Central

    Carrasco-Zevallos, Oscar M.; Keller, Brenton; Viehland, Christian; Shen, Liangbo; Seider, Michael I.; Izatt, Joseph A.; Toth, Cynthia A.

    2016-01-01

    Magnification of the surgical field using the operating microscope facilitated profound innovations in retinal surgery in the 1970s, such as pars plana vitrectomy. Although surgical instrumentation and illumination techniques are continually developing, the operating microscope for vitreoretinal procedures has remained essentially unchanged and currently limits the surgeon's depth perception and assessment of subtle microanatomy. Optical coherence tomography (OCT) has revolutionized clinical management of retinal pathology, and its introduction into the operating suite may have a similar impact on surgical visualization and treatment. In this article, we review the evolution of OCT for retinal surgery, from perioperative analysis to live volumetric (four-dimensional, 4D) image-guided surgery. We begin by briefly addressing the benefits and limitations of the operating microscope, the progression of OCT technology, and OCT applications in clinical/perioperative retinal imaging. Next, we review intraoperative OCT (iOCT) applications using handheld probes during surgical pauses, two-dimensional (2D) microscope-integrated OCT (MIOCT) of live surgery, and volumetric MIOCT of live surgery. The iOCT discussion focuses on technological advancements, applications during human retinal surgery, translational difficulties and limitations, and future directions. PMID:27409495

  15. Simultaneous real-time quantification of blood flow and vascular growth in the chick embryo using optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Kowalski, William J.; Teslovich, Nikola C.; Chen, Chia-Yuan; Keller, Bradley B.; Pekkan, Kerem

    2014-03-01

    Experimental and clinical data indicate that hemodynamic forces within the embryo provide critical biomechanical cues for cardiovascular morphogenesis, growth, and remodeling and that perturbed flow is a major etiology of congenital heart disease. However, embryonic flow-growth relationships are largely qualitative and poorly defined. In this work, we provide a quantitative analysis of in vivo flow and growth trends in the chick embryo using optical coherence tomography (OCT) to acquire simultaneous velocity and structural data of the right vitelline artery continuously over a ten hour period beginning at stage 16 (hour 54). We obtained 3D vessel volumes (15 μm lateral, 4.3 μm axial resolutions, 6 μm slice spacing) at 60 minute intervals, taking a B-scan time series totaling one cardiac cycle at each slice. Embryos were maintained at a constant 37°C and 60% humidity during the entire acquisition period through an inhouse built chamber. The 3D vessel lumen geometries were reconstructed manually to assess growth. Blood flow velocity was computed from the central B-scan using red blood cell particle image velocimetry. The use of extended OCT imaging as a non-invasive method for continuous and simultaneous flow and structural data can enhance our understanding of the biomechanical regulation of critical events in morphogenesis. Data acquired will be useful to validate predictive finite-element 3D growth models.

  16. Electrical resistance tomography for real-time mapping of the solid-liquid interface in tanks containing optically opaque fluids

    NASA Astrophysics Data System (ADS)

    Madupu, Amar; Mazumdar, Anindra; Zhang, Jinsong; Roelant, David; Srivastava, Rajiv

    2005-03-01

    The visualization of settled solid layers in vessels have many applications, of interest here is for facilitating the efficient retrieval of high-level radioactive waste (HLW) from underground storage tanks at Department of Energy sites. Visualization of the solids interface with opaque liquid above can"t be accomplished by regular optical imaging methods and hence our interest in using Electrical Resistance Tomography (ERT). The ideal arrangement for 3-D ERT imaging inside tanks is to use a multiple ring electrode system, which is complex and expensive. This research describes ERT imaging done with a single linear array as a benchmark study to ascertain the viability of its imaging of the interface. Experiments focused upon systematic analysis of many ERT tomograms of two simple settled solids layers (horizontal, 30o) using pulverized kaolin clay (10μdia) and water. Visualization was done using commercial ERT software. Injection current and electrode orientation were the two system parameters varied and analyzed. Reproducibility, accuracy and reliability of this ERT system will be presented.

  17. SU-E-J-127: Real-Time Dosimetric Assessment for Adaptive Head-And-Neck Treatment Via A GPU-Based Deformable Image Registration Framework

    SciTech Connect

    Qi, S; Neylon, J; Chen, A; Low, D; Kupelian, P; Steinberg, M; Santhanam, A

    2014-06-01

    Purposes: To systematically monitor anatomic variations and their dosimetric consequences during head-and-neck (H'N) radiation therapy using a GPU-based deformable image registration (DIR) framework. Methods: Eleven H'N IMRT patients comprised the subject population. The daily megavoltage CT and weekly kVCT scans were acquired for each patient. The pre-treatment CTs were automatically registered with their corresponding planning CT through an in-house GPU-based DIR framework. The deformation of each contoured structure was computed to account for non-rigid change in the patient setup. The Jacobian determinant for the PTVs and critical structures was used to quantify anatomical volume changes. Dose accumulation was performed to determine the actual delivered dose and dose accumulation. A landmark tool was developed to determine the uncertainty in the dose distribution due to registration error. Results: Dramatic interfraction anatomic changes leading to dosimetric variations were observed. During the treatment courses of 6–7 weeks, the parotid gland volumes changed up to 34.7%, the center-of-mass displacement of the two parotids varied in the range of 0.9–8.8mm. Mean doses were within 5% and 3% of the planned mean doses for all PTVs and CTVs, respectively. The cumulative minimum/mean/EUD doses were lower than the planned doses by 18%, 2%, and 7%, respectively for the PTV1. The ratio of the averaged cumulative cord maximum doses to the plan was 1.06±0.15. The cumulative mean doses assessed by the weekly kVCTs were significantly higher than the planned dose for the left-parotid (p=0.03) and right-parotid gland (p=0.006). The computation time was nearly real-time (∼ 45 seconds) for registering each pre-treatment CT to the planning CT and dose accumulation with registration accuracy (for kVCT) at sub-voxel level (<1.5mm). Conclusions: Real-time assessment of anatomic and dosimetric variations is feasible using the GPU-based DIR framework. Clinical implementation

  18. Toward a real time stand-off submillimeter-wave imaging system with large field of view: quasi-optical system design considerations

    NASA Astrophysics Data System (ADS)

    Gandini, Erio; Llombart, Nuria

    2015-05-01

    In the frame-work of the European project CONSORTIS, a stand-off system for concealed object detections working at submillimeter-wave frequencies is being developed. The system is required to perform real-time image acquisition over a large field of view at a short range using both an active and a passive sensor operating in the frequency range from 250 to 600 GHz. In this contribution, the main trade-offs associated with the quasi-optical system design are presented. The imaging distance is from 2 m to 5 m range with a spatial resolution lower than 2 cm. Focal plane arrays will be used to achieve high imaging frame rates. Two configurations are considered in CONSORTIS: a sparse array of active transceivers and incoherent passive staring array with a large number of elements. Both cases use mechanical scanning to achieve the required field of view. This paper presents an in-depth analysis of the different trade-offs driving the quasi-optical design: from the mechanical scanner considerations to the optical beam quality required over the whole field of view. This analysis starts from the fundamental limitations of the quasi-optical mechanical systems. The limitations of the optics are discussed considering a canonical elliptical reflector as a reference. After this fundamental analysis, we compare the performances of several practical standard implementations, based on dual-reflectors and lenses, with canonical geometries. It is shown that, at short ranges, the main limitation of the optical system is the poor beam quality associated with the wide angular field of view and none of the standard implementation fulfills the requirements. In the last section, a technique to overcome this limitation is investigated. In particular, the use of optics with oversized reflectors can significantly improve the performance over a larger field of view if the coma aberrations are limited by a good angular filter.

  19. In-situ, Real-Time Monitoring of Mechanical and Chemical Structure Changes in a V2O5 Battery Electrode Using a MEMS Optical Sensor

    SciTech Connect

    Jung, H.; Gerasopoulos, K.; Gnerlich, Markus; Talin, A. Alec; Ghodssi, Reza

    2014-06-01

    This work presents the first demonstration of a MEMS optical sensor for in-situ, real-time monitoring of both mechanical and chemical structure evolutions in a V2O5 lithium-ion battery (LIB) cathode during battery operation. A reflective membrane forms one side of a Fabry-Perot (FP) interferometer, while the other side is coated with V2O5 and exposed to electrolyte in a half-cell LIB. Using one microscope and two laser sources, both the induced membrane deflection and the corresponding Raman intensity changes are observed during lithium cycling. Results are in good agreement with the expected mechanical behavior and disorder change of the V2O5 layers, highlighting the significant potential of MEMS as enabling tools for advanced scientific investigations.

  20. The research and development of the adaptive optics in ophthalmology

    NASA Astrophysics Data System (ADS)

    Wu, Chuhan; Zhang, Xiaofang; Chen, Weilin

    2015-08-01

    Recently the combination of adaptive optics and ophthalmology has made great progress and become highly effective. The retina disease is diagnosed by retina imaging technique based on scanning optical system, so the scanning of eye requires optical system characterized by great ability of anti-moving and optical aberration correction. The adaptive optics possesses high level of adaptability and is available for real time imaging, which meets the requirement of medical retina detection with accurate images. Now the Scanning Laser Ophthalmoscope and the Optical Coherence Tomography are widely used, which are the core techniques in the area of medical retina detection. Based on the above techniques, in China, a few adaptive optics systems used for eye medical scanning have been designed by some researchers from The Institute of Optics And Electronics of CAS(The Chinese Academy of Sciences); some foreign research institutions have adopted other methods to eliminate the interference of eye moving and optical aberration; there are many relevant patents at home and abroad. In this paper, the principles and relevant technique details of the Scanning Laser Ophthalmoscope and the Optical Coherence Tomography are described. And the recent development and progress of adaptive optics in the field of eye retina imaging are analyzed and summarized.

  1. Real-time cosmology

    NASA Astrophysics Data System (ADS)

    Quercellini, Claudia; Amendola, Luca; Balbi, Amedeo; Cabella, Paolo; Quartin, Miguel

    2012-12-01

    In recent years, improved astrometric and spectroscopic techniques have opened the possibility of measuring the temporal change of radial and transverse position of sources in the sky over relatively short time intervals. This has made at least conceivable to establish a novel research domain, which we dub “real-time cosmology”. We review for the first time most of the work already done in this field, analysing the theoretical framework as well as some foreseeable observational strategies and their capability to constrain models. We first focus on real-time measurements of the overall redshift drift and angular separation shift in distant sources, which allows the observer to trace the background cosmic expansion and large scale anisotropy, respectively. We then examine the possibility of employing the same kind of observations to probe peculiar and proper accelerations in clustered systems, and therefore their gravitational potential. The last two sections are devoted to the future change of the cosmic microwave background on “short” time scales, as well as to the temporal shift of the temperature anisotropy power spectrum and maps. We conclude revisiting in this context the usefulness of upcoming experiments (like CODEX and Gaia) for real-time observations.

  2. Ultrahigh wavelength range (300nm-2μm) polarization-independent 500gs/s single-shot pulse, all-optical real time oscilloscope

    NASA Astrophysics Data System (ADS)

    Gleyze, Jean-François; Hocquet, Steve; Monnier Bourdin, Dominique; Le Boudec, Patrice; Arnaud, Romain; Chassagne, Bruno; Jolly, Alain; Penninckx, Denis

    2014-03-01

    The development of ultra-broadband oscilloscopes is mainly governed by the needs of future telecom networks. But other applications are requesting the availability of true real-time acquisition oscilloscopes. Systems able to be used in single-shot operation are of prime interest for Inertial Confinement Fusion (ICF) and for the related R&D for plasma physics. We previously demonstrate a single-shot, 100GHz design of an all-optical sampling oscilloscope at 1μm (MULO). This laboratory system has been improved in stability and compactness to make an all-in-one box prototype. More, by the addition of an opto-electro-optics (OEO) sub-system at the input, we developed the ability to use this oscilloscope to analyze an electrical input signal up to 60GHz. This new integrated subset also increases the range of wavelength for optical input signal, from 300nm up to 2μm. Furthermore, it allows the use of inexpensive opto-electronic components at telecom wavelength for this system regardless of the signal to be analysed. In parallel with these improvements, by optimizing the heart of the system, we get a very high sampling rate, up to 500Gs/s and more; this allows considering much higher bandwidths in the future. In this talk, we will present latest developments and integration of this system. It will also allow us to give more details on the innovative OEO sub-system.

  3. Optical coherence tomography for ultrahigh-resolution 3D imaging of cell development and real-time guiding for photodynamic therapy

    NASA Astrophysics Data System (ADS)

    Wang, Tianshi; Zhen, Jinggao; Wang, Bo; Xue, Ping

    2009-11-01

    Optical coherence tomography is a new emerging technique for cross-sectional imaging with high spatial resolution of micrometer scale. It enables in vivo and non-invasive imaging with no need to contact the sample and is widely used in biological and clinic application. In this paper optical coherence tomography is demonstrated for both biological and clinic applications. For biological application, a white-light interference microscope is developed for ultrahigh-resolution full-field optical coherence tomography (full-field OCT) to implement 3D imaging of biological tissue. Spatial resolution of 0.9μm×1.1μm (transverse×axial) is achieved A system sensitivity of 85 dB is obtained at an acquisition time of 5s per image. The development of a mouse embryo is studied layer by layer with our ultrahigh-resolution full-filed OCT. For clinic application, a handheld optical coherence tomography system is designed for real-time and in situ imaging of the port wine stains (PWS) patient and supplying surgery guidance for photodynamic therapy (PDT) treatment. The light source with center wavelength of 1310nm, -3 dB wavelength range of 90 nm and optical power of 9mw is utilized. Lateral resolution of 8 μm and axial resolution of 7μm at a rate of 2 frames per second and with 102dB sensitivity are achieved in biological tissue. It is shown that OCT images distinguish very well the normal and PWS tissues in clinic and are good to serve as a valuable diagnosis tool for PDT treatment.

  4. Adaptive optical zoom sensor.

    SciTech Connect

    Sweatt, William C.; Bagwell, Brett E.; Wick, David Victor

    2005-11-01

    In order to optically vary the magnification of an imaging system, continuous mechanical zoom lenses require multiple optical elements and use fine mechanical motion to precisely adjust the separations between individual or groups of lenses. By incorporating active elements into the optical design, we have designed and demonstrated imaging systems that are capable of variable optical magnification with no macroscopic moving parts. Changing the effective focal length and magnification of an imaging system can be accomplished by adeptly positioning two or more active optics in the optical design and appropriately adjusting the optical power of those elements. In this application, the active optics (e.g. liquid crystal spatial light modulators or deformable mirrors) serve as variable focal-length lenses. Unfortunately, the range over which currently available devices can operate (i.e. their dynamic range) is relatively small. Therefore, the key to this concept is to create large changes in the effective focal length of the system with very small changes in the focal lengths of individual elements by leveraging the optical power of conventional optical elements surrounding the active optics. By appropriately designing the optical system, these variable focal-length lenses can provide the flexibility necessary to change the overall system focal length, and therefore magnification, that is normally accomplished with mechanical motion in conventional zoom lenses.

  5. Real-Time Optical Diagnosis of the Rat Brain Exposed to a Laser-Induced Shock Wave: Observation of Spreading Depolarization, Vasoconstriction and Hypoxemia-Oligemia

    PubMed Central

    Sato, Shunichi; Kawauchi, Satoko; Okuda, Wataru; Nishidate, Izumi; Nawashiro, Hiroshi; Tsumatori, Gentaro

    2014-01-01

    Despite many efforts, the pathophysiology and mechanism of blast-induced traumatic brain injury (bTBI) have not yet been elucidated, partially due to the difficulty of real-time diagnosis and extremely complex factors determining the outcome. In this study, we topically applied a laser-induced shock wave (LISW) to the rat brain through the skull, for which real-time measurements of optical diffuse reflectance and electroencephalogram (EEG) were performed. Even under conditions showing no clear changes in systemic physiological parameters, the brain showed a drastic light scattering change accompanied by EEG suppression, which indicated the occurrence of spreading depression, long-lasting hypoxemia and signal change indicating mitochondrial energy impairment. Under the standard LISW conditions examined, hemorrhage and contusion were not apparent in the cortex. To investigate events associated with spreading depression, measurement of direct current (DC) potential, light scattering imaging and stereomicroscopic observation of blood vessels were also conducted for the brain. After LISW application, we observed a distinct negative shift in the DC potential, which temporally coincided with the transit of a light scattering wave, showing the occurrence of spreading depolarization and concomitant change in light scattering. Blood vessels in the brain surface initially showed vasodilatation for 3–4 min, which was followed by long-lasting vasoconstriction, corresponding to hypoxemia. Computer simulation based on the inverse Monte Carlo method showed that hemoglobin oxygen saturation declined to as low as ∼35% in the long-term hypoxemic phase. Overall, we found that topical application of a shock wave to the brain caused spreading depolarization/depression and prolonged severe hypoxemia-oligemia, which might lead to pathological conditions in the brain. Although further study is needed, our findings suggest that spreading depolarization/depression is one of the key

  6. Real-time optical diagnosis of the rat brain exposed to a laser-induced shock wave: observation of spreading depolarization, vasoconstriction and hypoxemia-oligemia.

    PubMed

    Sato, Shunichi; Kawauchi, Satoko; Okuda, Wataru; Nishidate, Izumi; Nawashiro, Hiroshi; Tsumatori, Gentaro

    2014-01-01

    Despite many efforts, the pathophysiology and mechanism of blast-induced traumatic brain injury (bTBI) have not yet been elucidated, partially due to the difficulty of real-time diagnosis and extremely complex factors determining the outcome. In this study, we topically applied a laser-induced shock wave (LISW) to the rat brain through the skull, for which real-time measurements of optical diffuse reflectance and electroencephalogram (EEG) were performed. Even under conditions showing no clear changes in systemic physiological parameters, the brain showed a drastic light scattering change accompanied by EEG suppression, which indicated the occurrence of spreading depression, long-lasting hypoxemia and signal change indicating mitochondrial energy impairment. Under the standard LISW conditions examined, hemorrhage and contusion were not apparent in the cortex. To investigate events associated with spreading depression, measurement of direct current (DC) potential, light scattering imaging and stereomicroscopic observation of blood vessels were also conducted for the brain. After LISW application, we observed a distinct negative shift in the DC potential, which temporally coincided with the transit of a light scattering wave, showing the occurrence of spreading depolarization and concomitant change in light scattering. Blood vessels in the brain surface initially showed vasodilatation for 3-4 min, which was followed by long-lasting vasoconstriction, corresponding to hypoxemia. Computer simulation based on the inverse Monte Carlo method showed that hemoglobin oxygen saturation declined to as low as ∼35% in the long-term hypoxemic phase. Overall, we found that topical application of a shock wave to the brain caused spreading depolarization/depression and prolonged severe hypoxemia-oligemia, which might lead to pathological conditions in the brain. Although further study is needed, our findings suggest that spreading depolarization/depression is one of the key events

  7. Real time Faraday spectrometer

    DOEpatents

    Smith, Jr., Tommy E.; Struve, Kenneth W.; Colella, Nicholas J.

    1991-01-01

    This invention uses a dipole magnet to bend the path of a charged particle beam. As the deflected particles exit the magnet, they are spatially dispersed in the bend-plane of the magnet according to their respective momenta and pass to a plurality of chambers having Faraday probes positioned therein. Both the current and energy distribution of the particles is then determined by the non-intersecting Faraday probes located along the chambers. The Faraday probes are magnetically isolated from each other by thin metal walls of the chambers, effectively providing real time current-versus-energy particle measurements.

  8. Net-zero Building Cluster Simulations and On-line Energy Forecasting for Adaptive and Real-Time Control and Decisions

    NASA Astrophysics Data System (ADS)

    Li, Xiwang

    Buildings consume about 41.1% of primary energy and 74% of the electricity in the U.S. Moreover, it is estimated by the National Energy Technology Laboratory that more than 1/4 of the 713 GW of U.S. electricity demand in 2010 could be dispatchable if only buildings could respond to that dispatch through advanced building energy control and operation strategies and smart grid infrastructure. In this study, it is envisioned that neighboring buildings will have the tendency to form a cluster, an open cyber-physical system to exploit the economic opportunities provided by a smart grid, distributed power generation, and storage devices. Through optimized demand management, these building clusters will then reduce overall primary energy consumption and peak time electricity consumption, and be more resilient to power disruptions. Therefore, this project seeks to develop a Net-zero building cluster simulation testbed and high fidelity energy forecasting models for adaptive and real-time control and decision making strategy development that can be used in a Net-zero building cluster. The following research activities are summarized in this thesis: 1) Development of a building cluster emulator for building cluster control and operation strategy assessment. 2) Development of a novel building energy forecasting methodology using active system identification and data fusion techniques. In this methodology, a systematic approach for building energy system characteristic evaluation, system excitation and model adaptation is included. The developed methodology is compared with other literature-reported building energy forecasting methods; 3) Development of the high fidelity on-line building cluster energy forecasting models, which includes energy forecasting models for buildings, PV panels, batteries and ice tank thermal storage systems 4) Small scale real building validation study to verify the performance of the developed building energy forecasting methodology. The outcomes of

  9. Real-time optical wireless transmissions of digital TV signals using white InGaN LEDs grown with an asymmetric quantum barrier.

    PubMed

    Tsai, Chia-Lung; Chen, Yen-Jen

    2015-10-19

    The feasibility of using InGaN LEDs grown with asymmetric barrier layer (ABL) as transmitters in visible light communications is investigated experimentally. Compared with normal LEDs, the improvement in the spontaneous emission rate due to enhanced carrier localization and better uniformity of carrier distribution in ABL-containing MQWs leads to the fabricated LEDs can exhibit a 32.6% (@ 350 mA) increase in emission intensity and a 10.5% increase in modulation bandwidth. After eliminating the slow-responding phosphorescent components emitting from the phosphor-converted white LEDs, an open eye-diagram at 180 Mb/s is demonstrated over a distance of 100 cm in directed line-of-sight optical links. With the use of proposed LEDs, real-time transmissions of digital TV signals over a moderate distance (~100 cm) in free space is shown to be available in a 150 Mbit/s white LED-based optical link with conventional on-off keying modulation.

  10. Development of a frameless stereotactic radiosurgery system based on real-time 6D position monitoring and adaptive head motion compensation

    NASA Astrophysics Data System (ADS)

    Wiersma, Rodney D.; Wen, Zhifei; Sadinski, Meredith; Farrey, Karl; Yenice, Kamil M.

    2010-01-01

    Stereotactic radiosurgery delivers radiation with great spatial accuracy. To achieve sub-millimeter accuracy for intracranial SRS, a head ring is rigidly fixated to the skull to create a fixed reference. For some patients, the invasiveness of the ring can be highly uncomfortable and not well tolerated. In addition, placing and removing the ring requires special expertise from a neurosurgeon, and patient setup time for SRS can often be long. To reduce the invasiveness, hardware limitations and setup time, we are developing a system for performing accurate head positioning without the use of a head ring. The proposed method uses real-time 6D optical position feedback for turning on and off the treatment beam (gating) and guiding a motor-controlled 3D head motion compensation stage. The setup consists of a central control computer, an optical patient motion tracking system and a 3D motion compensation stage attached to the front of the LINAC couch. A styrofoam head cast was custom-built for patient support and was mounted on the compensation stage. The motion feedback of the markers was processed by the control computer, and the resulting motion of the target was calculated using a rigid body model. If the target deviated beyond a preset position of 0.2 mm, an automatic position correction was performed with stepper motors to adjust the head position via the couch mount motion platform. In the event the target deviated more than 1 mm, a safety relay switch was activated and the treatment beam was turned off. The feasibility of the concept was tested using five healthy volunteers. Head motion data were acquired with and without the use of motion compensation over treatment times of 15 min. On average, test subjects exceeded the 0.5 mm tolerance 86% of the time and the 1.0 mm tolerance 45% of the time without motion correction. With correction, this percentage was reduced to 5% and 2% for the 0.5 mm and 1.0 mm tolerances, respectively.

  11. Adaptive Optics for Industry and Medicine

    NASA Astrophysics Data System (ADS)

    Dainty, Christopher

    2008-01-01

    at Iris AO (poster paper) / Michael A. Helmbrecht ... [et al.]. Electrostatic push pull mirror improvernents in visual optics (poster paper) / S. Bonora and L. Poletto. 25cm bimorph mirror for petawatt laser / S. Bonora ... [et al.]. Hysteresis compensation for piezo deformable mirror (poster paper) / H. Song ... [et al.]. Static and dynamic responses of an adaptive optics ferrofluidic mirror (poster paper) / A. Seaman ... [et al.]. New HDTV (1920 x 1080) phase-only SLM (poster paper) / Stefan Osten and Sven Krueger. Monomorph large aperture deformable mirror for laser applications (poster paper) / J-C Sinquin, J-M Lurcon, C. Guillemard. Low cost, high speed for adaptive optics control (oral paper) / Christopher D. Saunter and Gordon D. Love. Open loop woofer-tweeter adaptive control on the LAO multi-conjugate adaptive optics testbed (oral paper) / Edward Laag, Don Gavel and Mark Ammons -- pt. 2. Wavefront sensors. Wave front sensorless adaptive optics for imaging and microscopy (invited paper) / Martin J. Booth, Delphine Débarre and Tony Wilson. A fundamental limit for wavefront sensing (oral paper) / Carl Paterson. Coherent fibre-bundle wavefront sensor (oral paper) / Brian Vohnsen, I. Iglesias and Pablo Artal. Maximum-likelihood methods in wave-front sensing: nuisance parameters (oral paper) / David Lara, Harrison H. Barrett, and Chris Dainty. Real-time wavefront sensing for ultrafast high-power laser beams (oral paper) / Juan M. Bueno ... [et al.]. Wavefront sensing using a random phase screen (oral paper) / M. Loktev, G. Vdovin and O. Soloviev. Quadri-Wave Lateral Shearing Interferometry: a new mature technique for wave front sensing in adaptive optics (oral paper) / Benoit Wattellier ... [et al.]. In vivo measurement of ocular aberrations with a distorted grating wavefront sensor (oral paper) / P. Harrison ... [et al.]. Position-sensitive detector designed with unusual CMOS layout strategies for a Hartman-Shack wavefront sensor (oral Paper) / Davies W. de Lima

  12. Adaptive Optics for Large Telescopes

    SciTech Connect

    Olivier, S

    2008-06-27

    The use of adaptive optics was originally conceived by astronomers seeking to correct the blurring of images made with large telescopes due to the effects of atmospheric turbulence. The basic idea is to use a device, a wave front corrector, to adjust the phase of light passing through an optical system, based on some measurement of the spatial variation of the phase transverse to the light propagation direction, using a wave front sensor. Although the original concept was intended for application to astronomical imaging, the technique can be more generally applied. For instance, adaptive optics systems have been used for several decades to correct for aberrations in high-power laser systems. At Lawrence Livermore National Laboratory (LLNL), the world's largest laser system, the National Ignition Facility, uses adaptive optics to correct for aberrations in each of the 192 beams, all of which must be precisely focused on a millimeter scale target in order to perform nuclear physics experiments.

  13. Adaptation of a Phytophthora ramorum Real-Time Polymerase Chain Reaction Assay based on a mitochondrial gene region for use on the Cepheid SmartCycler

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Detection of Phytophthora ramorum (causal agent of sudden oak death) in U.S. commercial nurseries has led to quarantine regulations including inspection of nurseries in infested areas. Since P. ramorum can be difficult to culture from symptomatic tissue, methods such as real-time PCR provide rapid ...

  14. Real-time display with large field of view on fourier domain optical coherence tomography at 1310 nm wavelength for dermatology

    NASA Astrophysics Data System (ADS)

    Xiao, Qing; Hou, Jue; Fu, Ling

    2012-06-01

    A Fourier domain optical coherence tomography (OCT) system with 1310 nm light was demonstrated to study inflammatory human skin and the skin coated with a moisturizer in vivo. By using a graphics processing unit (GPU), the display rate could reach 20 frames/s with 1000 A-scans contained in one image. The field of view (FOV) of the cross-sectional image is 7 mm in the lateral direction and the penetration depth is ˜1 mm in skin. The result shows that, in inflammatory skin, the epidermis became thicker and had a decreased scattering; furthermore, the region of the severe lesion present an uneven thickness of the epidermis compared with the peripheral area. For the result of a finger tip coated with the moisturizer, the antireflection effect was significant and the stratum corneum became more transparent. In this letter, we demonstrated that real-time display with a large FOV could enable screening of a large tissue area; thereby increasing the dermatologic diagnostic potential of the method by permitting a comparison of the lesion and the normal peripheral region.

  15. Reflection-mode micro-spherical fiber-optic probes for in vitro real-time and single-cell level pH sensing.

    PubMed

    Yang, Qingbo; Wang, Hanzheng; Lan, Xinwei; Cheng, Baokai; Chen, Sisi; Shi, Honglan; Xiao, Hai; Ma, Yinfa

    2015-02-01

    pH sensing at the single-cell level without negatively affecting living cells is very important but still a remaining issue in the biomedical studies. A 70 μm reflection-mode fiber-optic micro-pH sensor was designed and fabricated by dip-coating thin layer of organically modified aerogel onto a tapered spherical probe head. A pH sensitive fluorescent dye 2', 7'-Bis (2-carbonylethyl)-5(6)-carboxyfluorescein (BCECF) was employed and covalently bonded within the aerogel networks. By tuning the alkoxide mixing ratio and adjusting hexamethyldisilazane (HMDS) priming procedure, the sensor can be optimized to have high stability and pH sensing ability. The in vitro real-time sensing capability was then demonstrated in a simple spectroscopic way, and showed linear measurement responses with a pH resolution up to an average of 0.049 pH unit within a narrow, but biological meaningful pH range of 6.12-7.81. Its novel characterizations of high spatial resolution, reflection mode operation, fast response and high stability, great linear response within biological meaningful pH range and high pH resolutions, make this novel pH probe a very cost-effective tool for chemical/biological sensing, especially within the single cell level research field.

  16. A Physics-Based Modeling and Real-Time Simulation of Biomechanical Diffusion Process Through Optical Imaged Alveolar Tissues on Graphical Processing Units

    NASA Astrophysics Data System (ADS)

    Kaya, Ilhan; Santhanam, Anand P.; Lee, Kye-Sung; Meemon, Panomsak; Papp, Nicolene; Rolland, Jannick P.

    Tissue engineering has broad applications from creating the much-needed engineered tissue and organ structures for regenerative medicine to providing in vitro testbeds for drug testing. In the latter, application domain, creating alveolar lung tissue, and simulating the diffusion process of oxygen and other possible agents from the air into the blood stream as well as modeling the removal of carbon dioxide and other possible entities from the blood stream are of critical importance to simulating lung functions in various environments. In this chapter, we propose a physics-based model to simulate the alveolar gas exchange and the alveolar process. Tissue engineers, for the first time, may utilize these simulation results to better understand the underlying gas exchange process and properly adjust the tissue growing cycles. In this work, alveolar tissues are imaged by means of an optical coherence microscopy (OCM) system developed in our laboratory. As a consequence, 3D alveoli tissue data with its inherent complex boundary is taken as input to the system, which is based on computational fluid mechanics in simulating the alveolar gas exchange. The visualization and the simulation of diffusion of the air into the blood through the alveoli tissue is performed using a state-of-art graphics processing unit (GPU). Results show the real-time simulation of the gas exchange through the 2D alveoli tissue.

  17. Real-time monitoring of benzene, toluene, and p-xylene in a photoreaction chamber with a tunable mid-infrared laser and ultraviolet differential optical absorption spectroscopy.

    PubMed

    Parsons, Matthew T; Sydoryk, Ihor; Lim, Alan; McIntyre, Thomas J; Tulip, John; Jäger, Wolfgang; McDonald, Karen

    2011-02-01

    We describe the implementation of a mid-infrared laser-based trace gas sensor with a photoreaction chamber, used for reproducing chemical transformations of benzene, toluene, and p-xylene (BTX) gases that may occur in the atmosphere. The system performance was assessed in the presence of photoreaction products including aerosol particles. A mid-infrared external cavity quantum cascade laser (EC-QCL)-tunable from 9.41-9.88 μm (1012-1063 cm(-1))-was used to monitor gas phase concentrations of BTX simultaneously and in real time during chemical processing of these compounds with hydroxyl radicals in a photoreaction chamber. Results are compared to concurrent measurements using ultraviolet differential optical absorption spectroscopy (UV DOAS). The EC-QCL based system provides quantitation limits of approximately 200, 200, and 600 parts in 10(9) (ppb) for benzene, toluene, and p-xylene, respectively, which represents a significant improvement over our previous work with this laser system. Correspondingly, we observe the best agreement between the EC-QCL measurements and the UV DOAS measurements with benzene, followed by toluene, then p-xylene. Although BTX gas-detection limits are not as low for the EC-QCL system as for UV DOAS, an unidentified by-product of the photoreactions was observed with the EC-QCL, but not with the UV DOAS system.

  18. Adsorption calorimetry during metal vapor deposition on single crystal surfaces: Increased flux, reduced optical radiation, and real-time flux and reflectivity measurements

    NASA Astrophysics Data System (ADS)

    Sellers, Jason R. V.; James, Trevor E.; Hemmingson, Stephanie L.; Farmer, Jason A.; Campbell, Charles T.

    2013-12-01

    Thin films of metals and other materials are often grown by physical vapor deposition. To understand such processes, it is desirable to measure the adsorption energy of the deposited species as the film grows, especially when grown on single crystal substrates where the structure of the adsorbed species, evolving interface, and thin film are more homogeneous and well-defined in structure. Our group previously described in this journal an adsorption calorimeter capable of such measurements on single-crystal surfaces under the clean conditions of ultrahigh vacuum [J. T. Stuckless, N. A. Frei, and C. T. Campbell, Rev. Sci. Instrum. 69, 2427 (1998)]. Here we describe several improvements to that original design that allow for heat measurements with ˜18-fold smaller standard deviation, greater absolute accuracy in energy calibration, and, most importantly, measurements of the adsorption of lower vapor-pressure materials which would have previously been impossible. These improvements are accomplished by: (1) using an electron beam evaporator instead of a Knudsen cell to generate the metal vapor at the source of the pulsed atomic beam, (2) changing the atomic beam design to decrease the relative amount of optical radiation that accompanies evaporation, (3) adding an off-axis quartz crystal microbalance for real-time measurement of the flux of the atomic beam during calorimetry experiments, and (4) adding capabilities for in situ relative diffuse optical reflectivity determinations (necessary for heat signal calibration). These improvements are not limited to adsorption calorimetry during metal deposition, but also could be applied to better study film growth of other elements and even molecular adsorbates.

  19. A Physics-Based Modeling and Real-Time Simulation of Biomechanical Diffusion Process Through Optical Imaged Alveolar Tissues on Graphical Processing Units

    NASA Astrophysics Data System (ADS)

    Kaya, Ilhan; Santhanam, Anand P.; Lee, Kye-Sung; Meemon, Panomsak; Papp, Nicolene; Rolland, Jannick P.

    Tissue engineering has broad applications from creating the much-needed engineered tissue and organ structures for regenerative medicine to providing in vitro testbeds for drug testing. In the latter, application domain, creating alveolar lung tissue, and simulating the diffusion process of oxygen and other possible agents from the air into the blood stream as well as modeling the removal of carbon dioxide and other possible entities from the blood stream are of critical importance to simulating lung functions in various environments. In this chapter, we propose a physics-based model to simulate the alveolar gas exchange and the alveolar diffusionDiffusion alveolar process. Tissue engineers, for the first time, may utilize these simulation results to better understand the underlying gas exchange process and properly adjust the tissue growing cycles. In this work, alveolar tissues are imaged by means of an optical coherence microscopyOptical coherence microscopy (OCM Modality OCM ) system developed in our laboratory. As a consequence, 3D alveoli tissue data with its inherent complex boundary is taken as input to the simulationSimulation diffusion system, which is based on computational fluid mechanics in simulating the alveolar gas exchange. The visualizationVisualization and the simulation of diffusion of the air into the blood through the alveoli tissue is performed using a state-of-art graphics processing unitGraphics processing unit (GPU). Results show the real-time simulation of the gas exchange through the 2D alveoli tissue.

  20. Near-Real-Time Monitoring and Reporting of Crop Growth Condition and Harvest Status Using an Integrated Optical and Radar Approach at the National-Scale in Canada

    NASA Astrophysics Data System (ADS)

    Shang, J.

    2015-12-01

    There has been an increasing need to have accurate and spatially detailed information on crop growth condition and harvest status over Canada's agricultural land so that the impacts of environmental conditions, market supply and demand, and transportation network limitations on crop production can be understood fully and acted upon in a timely manner. Presently, Canada doesn't have a national dataset that can provide near-real-time geospatial information on crop growth stage and harvest systematically so that reporting on risk events can be linked directly to the grain supply chain and crop production fluctuations. The intent of this study is to develop an integrated approach using Earth observation (EO) technology to provide a consistent, comprehensive picture of crop growth cycles (growth conditions and stages) and agricultural management activities (field preparation for seeding, harvest, and residue management). Integration of the optical and microwave satellite remote sensing technologies is imperative for robust methodology development and eventually for operational implementation. Particularly, the current synthetic aperture radar (SAR) system Radarsat-2 and to be launched Radarsat Constellation Mission (RCM) are unique EO resources to Canada. Incorporating these Canadian SAR resources with international SAR missions such as the Cosmesky-Med and TerraSAR, could be of great potential for developing change detection technologies particularly useful for monitoring harvest as well as other types of agricultural management events. The study revealed that radar and multi-scale (30m and 250m) optical satellite data can directly detect or infer 1) seeding date, 2) crop growth stages and gross primary productivity (GPP), and 3) harvest progress. Operational prototypes for providing growing-season information at the crop-specific level will be developed across the Canadian agricultural land base.

  1. Adsorption calorimetry during metal vapor deposition on single crystal surfaces: Increased flux, reduced optical radiation, and real-time flux and reflectivity measurements

    SciTech Connect

    Sellers, Jason R. V.; James, Trevor E.; Hemmingson, Stephanie L.; Farmer, Jason A.; Campbell, Charles T.

    2013-12-15

    Thin films of metals and other materials are often grown by physical vapor deposition. To understand such processes, it is desirable to measure the adsorption energy of the deposited species as the film grows, especially when grown on single crystal substrates where the structure of the adsorbed species, evolving interface, and thin film are more homogeneous and well-defined in structure. Our group previously described in this journal an adsorption calorimeter capable of such measurements on single-crystal surfaces under the clean conditions of ultrahigh vacuum [J. T. Stuckless, N. A. Frei, and C. T. Campbell, Rev. Sci. Instrum. 69, 2427 (1998)]. Here we describe several improvements to that original design that allow for heat measurements with ∼18-fold smaller standard deviation, greater absolute accuracy in energy calibration, and, most importantly, measurements of the adsorption of lower vapor-pressure materials which would have previously been impossible. These improvements are accomplished by: (1) using an electron beam evaporator instead of a Knudsen cell to generate the metal vapor at the source of the pulsed atomic beam, (2) changing the atomic beam design to decrease the relative amount of optical radiation that accompanies evaporation, (3) adding an off-axis quartz crystal microbalance for real-time measurement of the flux of the atomic beam during calorimetry experiments, and (4) adding capabilities for in situ relative diffuse optical reflectivity determinations (necessary for heat signal calibration). These improvements are not limited to adsorption calorimetry during metal deposition, but also could be applied to better study film growth of other elements and even molecular adsorbates.

  2. Real-time analysis keratometer

    NASA Technical Reports Server (NTRS)

    Adachi, Iwao P. (Inventor); Adachi, Yoshifumi (Inventor); Frazer, Robert E. (Inventor)

    1987-01-01

    A computer assisted keratometer in which a fiducial line pattern reticle illuminated by CW or pulsed laser light is projected on a corneal surface through lenses, a prismoidal beamsplitter quarterwave plate, and objective optics. The reticle surface is curved as a conjugate of an ideal corneal curvature. The fiducial image reflected from the cornea undergoes a polarization shift through the quarterwave plate and beamsplitter whereby the projected and reflected beams are separated and directed orthogonally. The reflected beam fiducial pattern forms a moire pattern with a replica of the first recticle. This moire pattern contains transverse aberration due to differences in curvature between the cornea and the ideal corneal curvature. The moire pattern is analyzed in real time by computer which displays either the CW moire pattern or a pulsed mode analysis of the transverse aberration of the cornea under observation, in real time. With the eye focused on a plurality of fixation points in succession, a survey of the entire corneal topography is made and a contour map or three dimensional plot of the cornea can be made as a computer readout in addition to corneal radius and refractive power analysis.

  3. High-resolution retinal imaging using adaptive optics and Fourier-domain optical coherence tomography

    DOEpatents

    Olivier, Scot S.; Werner, John S.; Zawadzki, Robert J.; Laut, Sophie P.; Jones, Steven M.

    2010-09-07

    This invention permits retinal images to be acquired at high speed and with unprecedented resolution in three dimensions (4.times.4.times.6 .mu.m). The instrument achieves high lateral resolution by using adaptive optics to correct optical aberrations of the human eye in real time. High axial resolution and high speed are made possible by the use of Fourier-domain optical coherence tomography. Using this system, we have demonstrated the ability to image microscopic blood vessels and the cone photoreceptor mosaic.

  4. Real time polarimetric dehazing.

    PubMed

    Mudge, Jason; Virgen, Miguel

    2013-03-20

    Remote sensing is a rich topic due to its utility in gathering detailed accurate information from locations that are not economically feasible traveling destinations or are physically inaccessible. However, poor visibility over long path lengths is problematic for a variety of reasons. Haze induced by light scatter is one cause for poor visibility and is the focus of this article. Image haze comes about as a result of light scattering off particles and into the imaging path causing a haziness to appear on the image. Image processing using polarimetric information of light scatter can be used to mitigate image haze. An imaging polarimeter which provides the Stokes values in real time combined with a "dehazing" algorithm can automate image haze removal for instant applications. Example uses are to improve visual display providing on-the-spot detection or imbedding in an active control loop to improve viewing and tracking while on a moving platform. In addition, removing haze in this manner allows the trade space for a system operational waveband to be opened up to bands which are object matched and not necessarily restricted by scatter effects.

  5. Coherent Digital Holographic Adaptive Optics

    NASA Astrophysics Data System (ADS)

    Liu, Changgeng

    A new type of adaptive optics (AO) based on the principles of digital holography (DH) is proposed and developed for the use in wide-field and confocal retinal imaging. Digital holographic adaptive optics (DHAO) dispenses with the wavefront sensor and wavefront corrector of the conventional AO system. DH is an emergent imaging technology that gives direct numerical access to the phase of the optical field, thus allowing precise control and manipulation of the optical field. Incorporation of DH in an ophthalmic imaging system can lead to versatile imaging capabilities at substantially reduced complexity and cost of the instrument. A typical conventional AO system includes several critical hardware pieces: spatial light modulator, lenslet array, and a second CCD camera in addition to the camera for imaging. The proposed DHAO system replaces these hardware components with numerical processing for wavefront measurement and compensation of aberration through the principles of DH. (Abstract shortened by UMI.).

  6. Real-time detection of small and dim moving objects in IR video sequences using a robust background estimator and a noise-adaptive double thresholding

    NASA Astrophysics Data System (ADS)

    Zingoni, Andrea; Diani, Marco; Corsini, Giovanni

    2016-10-01

    We developed an algorithm for automatically detecting small and poorly contrasted (dim) moving objects in real-time, within video sequences acquired through a steady infrared camera. The algorithm is suitable for different situations since it is independent of the background characteristics and of changes in illumination. Unlike other solutions, small objects of any size (up to single-pixel), either hotter or colder than the background, can be successfully detected. The algorithm is based on accurately estimating the background at the pixel level and then rejecting it. A novel approach permits background estimation to be robust to changes in the scene illumination and to noise, and not to be biased by the transit of moving objects. Care was taken in avoiding computationally costly procedures, in order to ensure the real-time performance even using low-cost hardware. The algorithm was tested on a dataset of 12 video sequences acquired in different conditions, providing promising results in terms of detection rate and false alarm rate, independently of background and objects characteristics. In addition, the detection map was produced frame by frame in real-time, using cheap commercial hardware. The algorithm is particularly suitable for applications in the fields of video-surveillance and computer vision. Its reliability and speed permit it to be used also in critical situations, like in search and rescue, defence and disaster monitoring.

  7. Manufacturing of the ESO adaptive optics facility

    NASA Astrophysics Data System (ADS)

    Arsenault, R.,; Madec, P.-Y.; Hubin, N.; Stroebele, S.; Paufique, J.; Vernet, E.; Hackenberg, W.; Pirard, J.-F.; Jochum, L.; Glindemann, A.; Jost, A.; Conzelmann, R.; Kiekebusch, M.; Tordo, S.; Lizon, J.-L.; Donaldson, R.; Fedrigo, E.; Soenke, C.; Duchateau, M.; Bruton, A.; Delabre, B.; Downing, M.; Reyes, J.; Kolb, J.; Bechet, C.; Lelouarn, M.; Bonaccini Calia, D.; Quattri, M.; Guidolin, I.; Buzzoni, B.; Dupuy, C.; Guzman, R.; Comin, M.; Silber, A.; Quentin, J.; La Penna, P.; Manescau, A.; Jolley, P.; Heinz, V.; Duhoux, P.; Argomedo, J.; Gallieni, D.; Lazzarini, P.; Biasi, R.; Andrighettoni, M.; Angerer, G.; Pescoller, D.; Stuik, R.,; Deep, A.

    2010-07-01

    The ESO Adaptive Optics Facility (AOF) consists in an evolution of one of the ESO VLT unit telescopes to a laser driven adaptive telescope with a deformable mirror in its optical train, in this case the secondary 1.1m mirror, and four Laser Guide Stars (LGSs). This evolution implements many challenging technologies like the Deformable Secondary Mirror (DSM) including a thin shell mirror (1.1 m diameter and 2mm thin), the high power Na lasers (20W), the low Read-Out Noise (RON) WaveFront Sensor (WFS) camera (< 1e-) and SPARTA the new generation of Real Time Computers (RTC) for adaptive control. It also faces many problematic similar to any Extremely Large Telescope (ELT) and as such, will validate many technologies and solutions needed for the European ELT (E-ELT) 42m telescope. The AOF will offer a very large (7 arcmin) Field Of View (FOV) GLAO correction in J, H and K bands (GRAAL+Hawk-I), a visible integral field spectrograph with a 1 arcmin GLAO corrected FOV (GALACSI-MUSE WFM) and finally a LTAO 7.5" FOV (GALACSI-MUSE NFM). Most systems of the AOF have completed final design and are in manufacturing phase. Specific activities are linked to the modification of the 8m telescope in order to accommodate the new DSM and the 4 LGS Units assembled on its Center-Piece. A one year test period in Europe is planned to test and validate all modes and their performance followed by a commissioning phase in Paranal scheduled for 2014.

  8. Real-time calibration-free C-scan images of the eye fundus using Master Slave swept source optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Bradu, Adrian; Kapinchev, Konstantin; Barnes, Fred; Garway-Heath, David F.; Rajendram, Ranjan; Keane, Pearce; Podoleanu, Adrian G.

    2015-03-01

    Recently, we introduced a novel Optical Coherence Tomography (OCT) method, termed as Master Slave OCT (MS-OCT), specialized for delivering en-face images. This method uses principles of spectral domain interfereometry in two stages. MS-OCT operates like a time domain OCT, selecting only signals from a chosen depth only while scanning the laser beam across the eye. Time domain OCT allows real time production of an en-face image, although relatively slowly. As a major advance, the Master Slave method allows collection of signals from any number of depths, as required by the user. The tremendous advantage in terms of parallel provision of data from numerous depths could not be fully employed by using multi core processors only. The data processing required to generate images at multiple depths simultaneously is not achievable with commodity multicore processors only. We compare here the major improvement in processing and display, brought about by using graphic cards. We demonstrate images obtained with a swept source at 100 kHz (which determines an acquisition time [Ta] for a frame of 200×200 pixels2 of Ta =1.6 s). By the end of the acquired frame being scanned, using our computing capacity, 4 simultaneous en-face images could be created in T = 0.8 s. We demonstrate that by using graphic cards, 32 en-face images can be displayed in Td 0.3 s. Other faster swept source engines can be used with no difference in terms of Td. With 32 images (or more), volumes can be created for 3D display, using en-face images, as opposed to the current technology where volumes are created using cross section OCT images.

  9. Simultaneous monitoring of biofilm growth, microbial activity, and inorganic deposits on surfaces with an in situ, online, real-time, non-destructive, optical sensor.

    PubMed

    Strathmann, Martin; Mittenzwey, Klaus-Henrik; Sinn, Gert; Papadakis, Wassilios; Flemming, Hans-Curt

    2013-01-01

    Deposits on surfaces in water-bearing systems, also known as 'fouling', can lead to substantial losses in the performance of industrial processes as well as a decreased product quality. Early detection and localization of such deposits can, to a considerable extent, save such losses. However, most of the surfaces that become fouled, for example, in process water pipes, membrane systems, power plants, and food and beverage industries, are difficult to access and analyses conducted on the water phase do not reveal the site or extent of deposits. Furthermore, it is of interest to distinguish biological from non-biological deposits. Although they usually occur together, different countermeasures are necessary. Therefore, sensors are required that indicate the development of surface fouling in real-time, non-destructively, and in situ, preferably allowing for discrimination between chemical and/or biological deposits. In this paper, an optical deposit sensor is presented which fulfills these requirements. Based on multiple fluorescence excitation emission matrix analysis, it detects autofluorescence of amino acids as indicators of biomass. Autofluorescence of nicotinamide adenine dinucleotide + hydrogen is interpreted as an indicator of biological activity, thus it acts as a viability marker, making the method suited for assessing the efficacy of disinfection treatments. Scattering signals from abiotic deposits such as calcium carbonate or corrosion products can clearly be distinguished from biotic substances and monitored separately. The sensor provides an early warning of fouling, allowing for timely countermeasures to be deployed. It also provides an assessment of the success of cleaning treatments and is a promising tool for integrated antifouling strategies.

  10. Task 1.12 - Solid-Phase Microextraction (SPME) coupled with optical spectroscopy for near-real-time determination of fuel and related pollutants in water samples

    SciTech Connect

    1995-02-01

    Measurement of organic water pollutants requires that samples be collected, transported, extracted (usually with large volumes of chlorinated solvents), and analyzed using chromatographic techniques. Such limitations make rapid determinations in the field very expensive, with analytical turnaround times of several days. Similar limitations exist for the analysis of organic air pollutants. An alternative approach is being developed that has been demonstrated to yield good sensitivities (ppb to ppm) and to achieve rapid (< 30 minutes) and inexpensive field surveys. This approach is based on the preconcentration of organic pollutants in optically clear sorbent media followed by measurement with either ultraviolet (UV) spectroscopy or Fourier transform infrared spectrometry (FT-IR). Investigations using sorbent media including methylsilicone gums (for UV spectroscopy) and Parafilm (for FT-IR) have shown that quantitative determinations of aromatic fuel components, such as benzene and alkylbenzenes, can be achieved using UV spectrometry, and of common solvents can be achieved using FT-IR. Sample concentration and spectroscopic steps can be performed in a few minutes using the combination of solid-phase sorption followed by spectroscopic measurements. This work has demonstrated that the approach can yield low-ppm to low-ppb detection limits for organic pollutants in water without the negative influences of water matrix components (e.g., dissolved humic material) that prohibit the use of direct spectroscopic measurements. A good potential for real-time air monitoring of organic pollutants also exists. Additional advantages of the technique are that no organic solvents are used and that a simple, very inexpensive dipstick device can be used with conventional (and portable) spectroscopic instrumentation. The results demonstrated that the method can be used as a solvent-free and rapid survey method in the lab or the field. 1 fig., 1 tab.

  11. Real-Time Keratometer

    NASA Technical Reports Server (NTRS)

    Frazer, Robert E.; Adachi, Iwao P.; Adachi, Yoshifumi

    1988-01-01

    Optical/electronic keratometer system produces contour map of corneal surface illuminated by infrared light pulses. Prevents operator error and eliminates need to apply fluorescent liquids to cornea. Keratometer provides both video display and numerical recording for corneal-transplant and radial-keratotomy surgery and for contact-lens fitting. Optical system of keratometer, circular grating pattern transmitted to corneal surface reflected onto identical circular grating on surface of fiber plate, producing Moire pattern. Data-analysis-and-display mode, laser light pulsed, and Moire pattern converted to digital information in frame grabber. Digital picture information sent to computer for processing and display.

  12. Real-Time Confocal Imaging Of The Living Eye

    NASA Astrophysics Data System (ADS)

    Jester, James V.; Cavanagh, H. Dwight; Essepian, John; Shields, William J.; Lemp, Michael A.

    1989-12-01

    In 1986, we adapted the Tandem Scanning Reflected Light Microscope of Petran and Hadraysky to permit non-invasive, confocal imaging of the living eye in real-time. We were first to obtain stable, confocal optical sections in vivo, from human and animal eyes. Using confocal imaging systems we have now studied living, normal volunteers, rabbits, cats and primates sequentially, non-invasively, and in real-time. The continued development of real-time confocal imaging systems will unlock the door to a new field of cell biology involving for the first time the study of dynamic cellular processes in living organ systems. Towards this end we have concentrated our initial studies on three areas (1) evaluation of confocal microscope systems for real-time image acquisition, (2) studies of the living normal cornea (epithelium, stroma, endothelium) in human and other species; and (3) sequential wound-healing responses in the cornea in single animals to lamellar-keratectomy injury (cellular migration, inflammation, scarring). We believe that this instrument represents an important, new paradigm for research in cell biology and pathology and that it will fundamentally alter all experimental and clinical approaches in future years.

  13. Towards real time speckle controlled retinal photocoagulation

    NASA Astrophysics Data System (ADS)

    Bliedtner, Katharina; Seifert, Eric; Stockmann, Leoni; Effe, Lisa; Brinkmann, Ralf

    2016-03-01

    Photocoagulation is a laser treatment widely used for the therapy of several retinal diseases. Intra- and inter-individual variations of the ocular transmission, light scattering and the retinal absorption makes it impossible to achieve a uniform effective exposure and hence a uniform damage throughout the therapy. A real-time monitoring and control of the induced damage is highly requested. Here, an approach to realize a real time optical feedback using dynamic speckle analysis is presented. A 532 nm continuous wave Nd:YAG laser is used for coagulation. During coagulation, speckle dynamics are monitored by a coherent object illumination using a 633nm HeNe laser and analyzed by a CMOS camera with a frame rate up to 1 kHz. It is obvious that a control system needs to determine whether the desired damage is achieved to shut down the system in a fraction of the exposure time. Here we use a fast and simple adaption of the generalized difference algorithm to analyze the speckle movements. This algorithm runs on a FPGA and is able to calculate a feedback value which is correlated to the thermal and coagulation induced tissue motion and thus the achieved damage. For different spot sizes (50-200 μm) and different exposure times (50-500 ms) the algorithm shows the ability to discriminate between different categories of retinal pigment epithelial damage ex-vivo in enucleated porcine eyes. Furthermore in-vivo experiments in rabbits show the ability of the system to determine tissue changes in living tissue during coagulation.

  14. Detection of Saharan dust and biomass burning events using near-real-time intensive aerosol optical properties in the north-western Mediterranean

    NASA Astrophysics Data System (ADS)

    Ealo, Marina; Alastuey, Andrés; Ripoll, Anna; Pérez, Noemí; Cruz Minguillón, María; Querol, Xavier; Pandolfi, Marco

    2016-10-01

    The study of Saharan dust events (SDEs) and biomass burning (BB) emissions are both topics of great scientific interest since they are frequent and important polluting scenarios affecting air quality and climate. The main aim of this work is evaluating the feasibility of using near-real-time in situ aerosol optical measurements for the detection of these atmospheric events in the western Mediterranean Basin (WMB). With this aim, intensive aerosol optical properties (SAE: scattering Ångström exponent, AAE: absorption Ångström exponent, SSAAE: single scattering albedo Ångström exponent and g: asymmetry parameter) were derived from multi-wavelength aerosol light scattering, hemispheric backscattering and absorption measurements performed at regional (Montseny; MSY, 720 m a.s.l.) and continental (Montsec; MSA, 1570 m a.s.l.) background sites in the WMB. A sensitivity study aiming at calibrating the measured intensive optical properties for SDEs and BB detection is presented and discussed. The detection of SDEs by means of the SSAAE parameter and Ångström matrix (made up by SAE and AAE) depended on the altitude of the measurement station and on SDE intensity. At MSA (mountain-top site) SSAAE detected around 85 % of SDEs compared with 50 % at the MSY station, where pollution episodes dominated by fine anthropogenic particles frequently masked the effect of mineral dust on optical properties during less intense SDEs. Furthermore, an interesting feature of SSAAE was its capability to detect the presence of mineral dust after the end of SDEs. Thus, resuspension processes driven by summer regional atmospheric circulations and dry conditions after SDEs favoured the accumulation of mineral dust at regional level having important consequences for air quality. On average, SAE, AAE and g ranged between -0.7 and 1, 1.3 and 2.5 and 0.5 and 0.75 respectively during SDEs. Based on the aethalometer model, BB contribution to equivalent black carbon (BC) accounted for 36 and 40

  15. Intelligent Optical Systems Using Adaptive Optics

    NASA Technical Reports Server (NTRS)

    Clark, Natalie

    2012-01-01

    Until recently, the phrase adaptive optics generally conjured images of large deformable mirrors being integrated into telescopes to compensate for atmospheric turbulence. However, the development of smaller, cheaper devices has sparked interest for other aerospace and commercial applications. Variable focal length lenses, liquid crystal spatial light modulators, tunable filters, phase compensators, polarization compensation, and deformable mirrors are becoming increasingly useful for other imaging applications including guidance navigation and control (GNC), coronagraphs, foveated imaging, situational awareness, autonomous rendezvous and docking, non-mechanical zoom, phase diversity, and enhanced multi-spectral imaging. The active components presented here allow flexibility in the optical design, increasing performance. In addition, the intelligent optical systems presented offer advantages in size and weight and radiation tolerance.

  16. Long-range, wide-field swept-source optical coherence tomography with GPU accelerated digital lock-in Doppler vibrography for real-time, in vivo middle ear diagnostics

    PubMed Central

    MacDougall, Dan; Farrell, Joshua; Brown, Jeremy; Bance, Manohar; Adamson, Robert

    2016-01-01

    We present the design, implementation and validation of a swept-source optical coherence tomography (OCT) system for real-time imaging of the human middle ear in live patients. Our system consists of a highly phase-stable Vernier-tuned distributed Bragg-reflector laser along with a real-time processing engine implemented on a graphics processing unit. We use the system to demonstrate, for the first time in live subjects, real-time Doppler measurements of middle ear vibration in response to sound, video rate 2D B-mode imaging of the middle ear and 3D volumetric B-mode imaging. All measurements were performed non-invasively through the intact tympanic membrane demonstrating that the technology is readily translatable to the clinic. PMID:27896001

  17. Real-time demonstration of 128-QAM-encoded optical OFDM transmission with a 5.25bit/s/Hz spectral efficiency in simple IMDD systems utilizing directly modulated DFB lasers.

    PubMed

    Jin, X Q; Giddings, R P; Hugues-Salas, E; Tang, J M

    2009-10-26

    The feasibility of implementing 128-QAM in off-the-shelf component-based real-time optical OFDM (OOFDM) transceivers incorporating advanced channel estimation, on-line performance monitoring and live parameter optimisation, is experimentally investigated, for the first time, in intensity-modulation and direct-detection (IMDD) single-mode fibre (SMF) and multi-mode fibre (MMF) transmission systems involving directly modulated DFB lasers. The highest ever spectral efficiency of 5.25bit/s/Hz is demonstrated successfully in the aforementioned simple systems. Experimental investigations show that, it is feasible to transmit 5.25 Gb/s 128-QAM-encoded OOFDM real-time signals over 25 km MetroCor(TM) SMFs and 500 m 62.5/125 microm OM1 MMFs. The impact of key parameters on the transmission performance of the real-time OOFDM transceivers with 128-QAM encoding are explored, based on which optimum signal clipping ratios are identified.

  18. Non-destructive optical clearing technique enhances optical coherence tomography (OCT) for real-time, 3D histomorphometry of brain tissue (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Paul, Akshay; Chang, Theodore H.; Chou, Li-Dek; Ramalingam, Tirunelveli S.

    2016-03-01

    Evaluation of neurodegenerative disease often requires examination of brain morphology. Volumetric analysis of brain regions and structures can be used to track developmental changes, progression of disease, and the presence of transgenic phenotypes. Current standards for microscopic investigation of brain morphology are limited to detection of superficial structures at a maximum depth of 300μm. While histological techniques can provide detailed cross-sections of brain structures, they require complicated tissue preparation and the ultimate destruction of the sample. A non-invasive, label-free imaging modality known as Optical Coherence Tomography (OCT) can produce 3-dimensional reconstructions through high-speed, cross-sectional scans of biological tissue. Although OCT allows for the preservation of intact samples, the highly scattering and absorbing properties of biological tissue limit imaging depth to 1-2mm. Optical clearing agents have been utilized to increase imaging depth by index matching and lipid digestion, however, these contemporary techniques are expensive and harsh on tissues, often irreversibly denaturing proteins. Here we present an ideal optical clearing agent that offers ease-of-use and reversibility. Similar to how SeeDB has been effective for microscopy, our fructose-based, reversible optical clearing technique provides improved OCT imaging and functional immunohistochemical mapping of disease. Fructose is a natural, non-toxic sugar with excellent water solubility, capable of increasing tissue transparency and reducing light scattering. We will demonstrate the improved depth-resolving performance of OCT for enhanced whole-brain imaging of normal and diseased murine brains following a fructose clearing treatment. This technique potentially enables rapid, 3-dimensional evaluation of biological tissues at axial and lateral resolutions comparable to histopathology.

  19. Structural mechanisms as revealed by real time mechano optical behavior of polylactic acid films in uni and biaxial deformation and heat setting processes

    NASA Astrophysics Data System (ADS)

    Ou, Xuesong

    In this study, structural development during PLA film processing was investigated with a new instrumented biaxial stretcher capable of real time monitoring of true stress, true strain and in-plane as well as out-of-plane birefringence under fast deformation rates. The effects of stretching rate and mode on mechano-optical behaviors and correspondent morphology development were investigated. At low deformation levels, a linear regime I associated with orientation of amorphous chains was observed in all modes of deformation. Following regime I, a steeper regime II associated with stress induced crystallization was observed during uniaxial constrained width (UCW) stretching under low rates before birefringence begins to level off in regime III due to finite extensibility of chains. During UCW stretching under high rate, regime I transformed directly into regime III, and this is associated with the formation of a very stable nematic mesophase. Direct transformation from regime I to regime III is observed during simultaneous biaxial (SIM) stretching under all rates. The kinetics of structural changes during heat setting from a pre-oriented state was investigated by rapid tracking of in and out-of-plane birefringence of pre-oriented films with a new instrumented annealing chamber capable of fast sample insertion and removal. Development of birefringence, which reflects overall chain orientation, and associated structural evolution during constrained annealing of extended PLA films were clarified. Structural evolution is determined by the competition between chain relaxation and registration of segments into well oriented nuclei that grow during annealing, leading to formation of a long range network of chains that arrests the chains in their oriented state. At low deformation the temporal evolution of birefringence first involves relaxation followed by a rapid increase associated with crystallization. The initial relaxation disappears with increase in deformation in the

  20. Precision Targeting with a Tracking adaptive Optics Scanning Laser Ophthalmoscope

    DTIC Science & Technology

    2006-02-01

    in Figure 2) but drives two galvanometers placed at appropriate conjugates within the path of the adaptive optics scanning laser ophthalmoscope...reflectometer. The input to the "slave" control loop is the scaled position signals from the master galvanometers . The slave tracking mirrors are placed at...signals and drive galvanometers . The DSP has a loop rate of 62.5 kHz (compared to 16 kHz in the previously-used real-time processing board) for a

  1. Quantification of meat proportions by measuring DNA contents in raw and boiled sausages using matrix-adapted calibrators and multiplex real-time PCR.

    PubMed

    Köppel, René; Eugster, Albert; Ruf, Jürg; Rentsch, Jürg

    2012-01-01

    The quantification of meat proportions in raw and boiled sausage according to the recipe was evaluated using three different calibrators. To measure the DNA contents from beef, pork, sheep (mutton), and horse, a tetraplex real-time PCR method was applied. Nineteen laboratories analyzed four meat products each made of different proportions of beef, pork, sheep, and horse meat. Three kinds of calibrators were used: raw and boiled sausages of known proportions ranging from 1 to 55% of meat, and a dilution series of DNA from muscle tissue. In general, results generated using calibration sausages were more accurate than those resulting from the use of DNA from muscle tissue, and exhibited smaller measurement uncertainties. Although differences between uses of raw and boiled calibration sausages were small, the most precise and accurate results were obtained by calibration with fine-textured boiled reference sausages.

  2. Real-Time Observation of Antimicrobial Polycation Effects on Escherichia coli: Adapting the Carpet Model for Membrane Disruption to Quaternary Copolyoxetanes.

    PubMed

    Wang, Congzhou; Zolotarskaya, Olga Y; Nair, Sithara S; Ehrhardt, Christopher J; Ohman, Dennis E; Wynne, Kenneth J; Yadavalli, Vamsi K

    2016-03-29

    Real-time atomic force microscopy (AFM) was used for analyzing effects of the antimicrobial polycation copolyoxetane P[(C12)-(ME2Ox)-50/50], C12-50 on the membrane of a model bacterium, Escherichia coli (ATCC# 35218). AFM imaging showed cell membrane changes with increasing C12-50 concentration and time including nanopore formation and bulges associated with outer bacterial membrane disruption. A macroscale bactericidal concentration study for C12-50 showed a 4 log kill at 15 μg/mL with conditions paralleling imaging (1 h, 1x PBS, physiological pH, 25 °C). The dramatic changes from the control image to 1 h after introducing 15 μg/mL C12-50 are therefore reasonably attributed to cell death. At the highest concentration (60 μg/mL) further cell membrane disruption results in leakage of cytoplasm driven by detergent-like action. The sequence of processes for initial membrane disruption by the synthetic polycation C12-50 follows the carpet model posited for antimicrobial peptides (AMPs). However, the nanoscale details are distinctly different as C12-50 is a synthetic, water-soluble copolycation that is best modeled as a random coil. In a complementary AFM study, chemical force microscopy shows that incubating cells with C12-50 decreased the hydrophobicity across the entire cell surface at an early stage. This finding provides additional evidence indicating that C12-50 polycations initially bind with the cell membrane in a carpet-like fashion. Taken together, real time AFM imaging elucidates the mechanism of antimicrobial action for copolyoxetane C12-50 at the single cell level. In future work this approach will provide important insights into structure-property relationships and improved antimicrobial effectiveness for synthetic amphiphilic polycations.

  3. Adaptive optics optical coherence tomography in glaucoma.

    PubMed

    Dong, Zachary M; Wollstein, Gadi; Wang, Bo; Schuman, Joel S

    2017-03-01

    Since the introduction of commercial optical coherence tomography (OCT) systems, the ophthalmic imaging modality has rapidly expanded and it has since changed the paradigm of visualization of the retina and revolutionized the management and diagnosis of neuro-retinal diseases, including glaucoma. OCT remains a dynamic and evolving imaging modality, growing from time-domain OCT to the improved spectral-domain OCT, adapting novel image analysis and processing methods, and onto the newer swept-source OCT and the implementation of adaptive optics (AO) into OCT. The incorporation of AO into ophthalmic imaging modalities has enhanced OCT by improving image resolution and quality, particularly in the posterior segment of the eye. Although OCT previously captured in-vivo cross-sectional images with unparalleled high resolution in the axial direction, monochromatic aberrations of the eye limit transverse or lateral resolution to about 15-20 μm and reduce overall image quality. In pairing AO technology with OCT, it is now possible to obtain diffraction-limited resolution images of the optic nerve head and retina in three-dimensions, increasing resolution down to a theoretical 3 μm(3). It is now possible to visualize discrete structures within the posterior eye, such as photoreceptors, retinal nerve fiber layer bundles, the lamina cribrosa, and other structures relevant to glaucoma. Despite its limitations and barriers to widespread commercialization, the expanding role of AO in OCT is propelling this technology into clinical trials and onto becoming an invaluable modality in the clinician's arsenal.

  4. In vivo imaging of human photoreceptor mosaic with wavefront sensorless adaptive optics optical coherence tomography.

    PubMed

    Wong, Kevin S K; Jian, Yifan; Cua, Michelle; Bonora, Stefano; Zawadzki, Robert J; Sarunic, Marinko V

    2015-02-01

    Wavefront sensorless adaptive optics optical coherence tomography (WSAO-OCT) is a novel imaging technique for in vivo high-resolution depth-resolved imaging that mitigates some of the challenges encountered with the use of sensor-based adaptive optics designs. This technique replaces the Hartmann Shack wavefront sensor used to measure aberrations with a depth-resolved image-driven optimization algorithm, with the metric based on the OCT volumes acquired in real-time. The custom-built ultrahigh-speed GPU processing platform and fast modal optimization algorithm presented in this paper was essential in enabling real-time, in vivo imaging of human retinas with wavefront sensorless AO correction. WSAO-OCT is especially advantageous for developing a clinical high-resolution retinal imaging system as it enables the use of a compact, low-cost and robust lens-based adaptive optics design. In this report, we describe our WSAO-OCT system for imaging the human photoreceptor mosaic in vivo. We validated our system performance by imaging the retina at several eccentricities, and demonstrated the improvement in photoreceptor visibility with WSAO compensation.

  5. In vivo imaging of human photoreceptor mosaic with wavefront sensorless adaptive optics optical coherence tomography

    PubMed Central

    Wong, Kevin S. K.; Jian, Yifan; Cua, Michelle; Bonora, Stefano; Zawadzki, Robert J.; Sarunic, Marinko V.

    2015-01-01

    Wavefront sensorless adaptive optics optical coherence tomography (WSAO-OCT) is a novel imaging technique for in vivo high-resolution depth-resolved imaging that mitigates some of the challenges encountered with the use of sensor-based adaptive optics designs. This technique replaces the Hartmann Shack wavefront sensor used to measure aberrations with a depth-resolved image-driven optimization algorithm, with the metric based on the OCT volumes acquired in real-time. The custom-built ultrahigh-speed GPU processing platform and fast modal optimization algorithm presented in this paper was essential in enabling real-time, in vivo imaging of human retinas with wavefront sensorless AO correction. WSAO-OCT is especially advantageous for developing a clinical high-resolution retinal imaging system as it enables the use of a compact, low-cost and robust lens-based adaptive optics design. In this report, we describe our WSAO-OCT system for imaging the human photoreceptor mosaic in vivo. We validated our system performance by imaging the retina at several eccentricities, and demonstrated the improvement in photoreceptor visibility with WSAO compensation. PMID:25780747

  6. Extreme Adaptive Optics Planet Imager

    NASA Astrophysics Data System (ADS)

    Macintosh, B.; Graham, J. R.; Ghez, A.; Kalas, P.; Lloyd, J.; Makidon, R.; Olivier, S.; Patience, J.; Perrin, M.; Poyneer, L.; Severson, S.; Sheinis, A.; Sivaramakrishnan, A.; Troy, M.; Wallace, J.; Wilhelmsen, J.

    2002-12-01

    Direct detection of photons emitted or reflected by extrasolar planets is the next major step in extrasolar planet studies. Current adaptive optics (AO) systems, with <300 subapertures and Strehl ratio 0.4-0.7, can achieve contrast levels of 106 at 2" separations; this is sufficient to see very young planets in wide orbits but insufficient to detect solar systems more like our own. Contrast levels of 107 - 108 in the near-IR are needed to probe a significant part of the extrasolar planet phase space. The NSF Center for Adaptive Optics is carrying out a design study for a dedicated ultra-high-contrast "Extreme" adaptive optics system for an 8-10m telescope. With 3000 controlled subapertures it should achieve Strehl ratios > 0.9 in the near-IR. Using a spatially filtered wavefront sensor, the system will be optimized to control scattered light over a large radius and suppress artifacts caused static errors. We predict that it will achieve contrast levels of 107-108 around a large sample of stars (R<7-10), sufficient to detect Jupiter-like planets through their near-IR emission over a wide range of ages and masses. The system will be capable of a variety of high-contrast science including studying circumstellar dust disks at densities a factor of 10-100 lower than currently feasible and a systematic inventory of other solar systems on 10-100 AU scale. This work was supported by the NSF Science and Technology Center for Adaptive Optics, managed by UC Santa Cruz under AST-9876783. Portions of this work was performed under the auspices of the U.S. Department of Energy, under contract No. W-7405-Eng-48.

  7. Exploiting Adaptive Optics with Deformable Secondary Mirrors

    DTIC Science & Technology

    2007-03-08

    progress in tomographic wavefront sensing and altitude conjugated adaptive correction, and is a critical step forward for adaptive optics for future large...geostationary satellites, captured at the 6.5 m MMT telescope, using the deformable secondary adaptive optics system....new technology to the unique development of deformable secondary mirrors pioneered at the University of Arizona’s Center for Astronomical Adaptive

  8. Sparse-aperture adaptive optics

    NASA Astrophysics Data System (ADS)

    Tuthill, Peter; Lloyd, James; Ireland, Michael; Martinache, Frantz; Monnier, John; Woodruff, Henry; ten Brummelaar, Theo; Turner, Nils; Townes, Charles

    2006-06-01

    Aperture masking interferometry and Adaptive Optics (AO) are two of the competing technologies attempting to recover diffraction-limited performance from ground-based telescopes. However, there are good arguments that these techniques should be viewed as complementary, not competitive. Masking has been shown to deliver superior PSF calibration, rejection of atmospheric noise and robust recovery of phase information through the use of closure phases. However, this comes at the penalty of loss of flux at the mask, restricting the technique to bright targets. Adaptive optics, on the other hand, can reach a fainter class of objects but suffers from the difficulty of calibration of the PSF which can vary with observational parameters such as seeing, airmass and source brightness. Here we present results from a fusion of these two techniques: placing an aperture mask downstream of an AO system. The precision characterization of the PSF enabled by sparse-aperture interferometry can now be applied to deconvolution of AO images, recovering structure from the traditionally-difficult regime within the core of the AO-corrected transfer function. Results of this program from the Palomar and Keck adaptive optical systems are presented.

  9. Contrast-based sensorless adaptive optics for retinal imaging.

    PubMed

    Zhou, Xiaolin; Bedggood, Phillip; Bui, Bang; Nguyen, Christine T O; He, Zheng; Metha, Andrew

    2015-09-01

    Conventional adaptive optics ophthalmoscopes use wavefront sensing methods to characterize ocular aberrations for real-time correction. However, there are important situations in which the wavefront sensing step is susceptible to difficulties that affect the accuracy of the correction. To circumvent these, wavefront sensorless adaptive optics (or non-wavefront sensing AO; NS-AO) imaging has recently been developed and has been applied to point-scanning based retinal imaging modalities. In this study we show, for the first time, contrast-based NS-AO ophthalmoscopy for full-frame in vivo imaging of human and animal eyes. We suggest a robust image quality metric that could be used for any imaging modality, and test its performance against other metrics using (physical) model eyes.

  10. Contrast-based sensorless adaptive optics for retinal imaging

    PubMed Central

    Zhou, Xiaolin; Bedggood, Phillip; Bui, Bang; Nguyen, Christine T.O.; He, Zheng; Metha, Andrew

    2015-01-01

    Conventional adaptive optics ophthalmoscopes use wavefront sensing methods to characterize ocular aberrations for real-time correction. However, there are important situations in which the wavefront sensing step is susceptible to difficulties that affect the accuracy of the correction. To circumvent these, wavefront sensorless adaptive optics (or non-wavefront sensing AO; NS-AO) imaging has recently been developed and has been applied to point-scanning based retinal imaging modalities. In this study we show, for the first time, contrast-based NS-AO ophthalmoscopy for full-frame in vivo imaging of human and animal eyes. We suggest a robust image quality metric that could be used for any imaging modality, and test its performance against other metrics using (physical) model eyes. PMID:26417525

  11. An accurate, flexible and small optical fiber sensor: a novel technological breakthrough for real-time analysis of dynamic blood flow data in vivo.

    PubMed

    Yuan, Qiao-ying; Zhang, Ling; Xiao, Dan; Zhao, Kun; Lin, Chun; Si, Liang-yi

    2014-01-01

    Because of the limitations of existing methods and techniques for directly obtaining real-time blood data, no accurate microflow in vivo real-time analysis method exists. To establish a novel technical platform for real-time in vivo detection and to analyze average blood pressure and other blood flow parameters, a small, accurate, flexible, and nontoxic Fabry-Perot fiber sensor was designed. The carotid sheath was implanted through intubation of the rabbit carotid artery (n = 8), and the blood pressure and other detection data were determined directly through the veins. The fiber detection results were compared with test results obtained using color Doppler ultrasound and a physiological pressure sensor recorder. Pairwise comparisons among the blood pressure results obtained using the three methods indicated that real-time blood pressure information obtained through the fiber sensor technique exhibited better correlation than the data obtained with the other techniques. The highest correlation (correlation coefficient of 0.86) was obtained between the fiber sensor and pressure sensor. The blood pressure values were positively related to the total cholesterol level, low-density lipoprotein level, number of red blood cells, and hemoglobin level, with correlation coefficients of 0.033, 0.129, 0.358, and 0.373, respectively. The blood pressure values had no obvious relationship with the number of white blood cells and high-density lipoprotein and had a negative relationship with triglyceride levels, with a correlation coefficient of -0.031. The average ambulatory blood pressure measured by the fiber sensor exhibited a negative correlation with the quantity of blood platelets (correlation coefficient of -0.839, P<0.05). The novel fiber sensor can thus obtain in vivo blood pressure data accurately, stably, and in real time; the sensor can also determine the content and status of the blood flow to some extent. Therefore, the fiber sensor can obtain partially real-time

  12. An Accurate, Flexible and Small Optical Fiber Sensor: A Novel Technological Breakthrough for Real-Time Analysis of Dynamic Blood Flow Data In Vivo

    PubMed Central

    Yuan, Qiao-ying; Zhang, Ling; Xiao, Dan; Zhao, Kun; Lin, Chun; Si, Liang-yi

    2014-01-01

    Because of the limitations of existing methods and techniques for directly obtaining real-time blood data, no accurate microflow in vivo real-time analysis method exists. To establish a novel technical platform for real-time in vivo detection and to analyze average blood pressure and other blood flow parameters, a small, accurate, flexible, and nontoxic Fabry-Perot fiber sensor was designed. The carotid sheath was implanted through intubation of the rabbit carotid artery (n = 8), and the blood pressure and other detection data were determined directly through the veins. The fiber detection results were compared with test results obtained using color Doppler ultrasound and a physiological pressure sensor recorder. Pairwise comparisons among the blood pressure results obtained using the three methods indicated that real-time blood pressure information obtained through the fiber sensor technique exhibited better correlation than the data obtained with the other techniques. The highest correlation (correlation coefficient of 0.86) was obtained between the fiber sensor and pressure sensor. The blood pressure values were positively related to the total cholesterol level, low-density lipoprotein level, number of red blood cells, and hemoglobin level, with correlation coefficients of 0.033, 0.129, 0.358, and 0.373, respectively. The blood pressure values had no obvious relationship with the number of white blood cells and high-density lipoprotein and had a negative relationship with triglyceride levels, with a correlation coefficient of –0.031. The average ambulatory blood pressure measured by the fiber sensor exhibited a negative correlation with the quantity of blood platelets (correlation coefficient of −0.839, P<0.05). The novel fiber sensor can thus obtain in vivo blood pressure data accurately, stably, and in real time; the sensor can also determine the content and status of the blood flow to some extent. Therefore, the fiber sensor can obtain partially real-time

  13. Applications of real-time holography

    NASA Astrophysics Data System (ADS)

    Feinberg, J.

    1985-01-01

    Holographic principles are discussed, with a description of the steps of hologram production and reconstruction and the materials used in storage of the images. Real-time holography applications, including photolithography, double-exposure interferometry, time-averaged holography, parallel optical processing, optical phase conjugation, beam steering, and moving holograms, are described in detail. In addition, current areas of active research and some experimental applications are discussed.

  14. Real-time monitoring system for microfluidics

    NASA Astrophysics Data System (ADS)

    Sapuppo, F.; Cantelli, G.; Fortuna, L.; Arena, P.; Bucolo, M.

    2007-05-01

    A new non-invasive real-time system for the monitoring and control of microfluidodynamic phenomena is proposed. The general purpose design of such system is suitable for in vitro and in vivo experimental setup and therefore for microfluidic application in the biomedical field such as lab-on-chip and for research studies in the field of microcirculation. The system consists of an ad hoc optical setup for image magnification providing images suitable for image acquisition and processing. The optic system was designed and developed using discrete opto-mechanic components mounted on a breadboard in order to provide an optic path accessible at any point where the information needs to be acquired. The optic sensing, acquisition, and processing were performed using an integrated vision system based on the Cellular Nonlinear Networks (CNNs) analogic technology called Focal Plane Processor (FPP, Eye-RIS, Anafocus) and inserted in the optic path. Ad hoc algorithms were implemented for the real-time analysis and extraction of fluido-dynamic parameters in micro-channels. They were tested on images recorded during in vivo microcirculation experiments on hamsters and then they were applied on images optically acquired and processed in real-time during in vitro experiments on a continuous microfluidic device (serpentine mixer, ThinXXS) with a two-phase fluid.

  15. Real-time flutter identification

    NASA Technical Reports Server (NTRS)

    Roy, R.; Walker, R.

    1985-01-01

    The techniques and a FORTRAN 77 MOdal Parameter IDentification (MOPID) computer program developed for identification of the frequencies and damping ratios of multiple flutter modes in real time are documented. Physically meaningful model parameterization was combined with state of the art recursive identification techniques and applied to the problem of real time flutter mode monitoring. The performance of the algorithm in terms of convergence speed and parameter estimation error is demonstrated for several simulated data cases, and the results of actual flight data analysis from two different vehicles are presented. It is indicated that the algorithm is capable of real time monitoring of aircraft flutter characteristics with a high degree of reliability.

  16. Acousto-Optic Adaptive Processing (AOAP).

    DTIC Science & Technology

    1983-12-01

    I ~.sls Phe Report December 1963 •- ACOUSTO - OPTIC ADAPTIVE <PROCESSING (AOAP) General Electric Company W. A. Penn, D. R. Morgan, A. Aridgides and M. L...numnber) Optical signal processing Acousto - optical modulators Adaptive signal processing - Adaptive sidelobe cancellation 20. ABSTRACT (Contnue an...required operations of multiplication and time delay are provided by acousto - optical (AO) delay lines. The required time integraticO is provided by

  17. Test Target for Adaptive Optics.

    DTIC Science & Technology

    adaptive optics comprising, in the preferred embodiment, a plurality of nine adjacent, stacked, and aligned rows of a multiplicity of alternate opaque sections and transparent sections in a repeating bar pattern, with all sections being positioned on a flat transparent medium (such as film or glass), and with each opaque section being an opaque bar and with each transparent section being a transparent bar. Each row has a different spatial frequency than any other of the nine rows, with the spatial frequency of any one row being of a different multiple of the row having the

  18. Real-time software receiver

    NASA Technical Reports Server (NTRS)

    Ledvina, Brent M. (Inventor); Psiaki, Mark L. (Inventor); Powell, Steven P. (Inventor); Kintner, Jr., Paul M. (Inventor)

    2006-01-01

    A real-time software receiver that executes on a general purpose processor. The software receiver includes data acquisition and correlator modules that perform, in place of hardware correlation, baseband mixing and PRN code correlation using bit-wise parallelism.

  19. Real-time software receiver

    NASA Technical Reports Server (NTRS)

    Ledvina, Brent M. (Inventor); Psiaki, Mark L. (Inventor); Powell, Steven P. (Inventor); Kintner, Jr., Paul M. (Inventor)

    2007-01-01

    A real-time software receiver that executes on a general purpose processor. The software receiver includes data acquisition and correlator modules that perform, in place of hardware correlation, baseband mixing and PRN code correlation using bit-wise parallelism.

  20. Real Time Data System (RTDS)

    NASA Technical Reports Server (NTRS)

    Muratore, John F.

    1991-01-01

    Lessons learned from operational real time expert systems are examined. The basic system architecture is discussed. An expert system is any software that performs tasks to a standard that would normally require a human expert. An expert system implies knowledge contained in data rather than code. And an expert system implies the use of heuristics as well as algorithms. The 15 top lessons learned by the operation of a real time data system are presented.

  1. Real-time vision systems

    SciTech Connect

    Johnson, R.; Hernandez, J.E.; Lu, Shin-yee

    1994-11-15

    Many industrial and defence applications require an ability to make instantaneous decisions based on sensor input of a time varying process. Such systems are referred to as `real-time systems` because they process and act on data as it occurs in time. When a vision sensor is used in a real-time system, the processing demands can be quite substantial, with typical data rates of 10-20 million samples per second. A real-time Machine Vision Laboratory (MVL) was established in FY94 to extend our years of experience in developing computer vision algorithms to include the development and implementation of real-time vision systems. The laboratory is equipped with a variety of hardware components, including Datacube image acquisition and processing boards, a Sun workstation, and several different types of CCD cameras, including monochrome and color area cameras and analog and digital line-scan cameras. The equipment is reconfigurable for prototyping different applications. This facility has been used to support several programs at LLNL, including O Division`s Peacemaker and Deadeye Projects as well as the CRADA with the U.S. Textile Industry, CAFE (Computer Aided Fabric Inspection). To date, we have successfully demonstrated several real-time applications: bullet tracking, stereo tracking and ranging, and web inspection. This work has been documented in the ongoing development of a real-time software library.

  2. Statistical evaluation of the performance of an optimized adaptive optics arm for retinal imaging flood system

    NASA Astrophysics Data System (ADS)

    Magaña Chávez, J. L.; Medina-Márquez, J.; Valdivieso-González, L. G.; Balderas-Mata, S. E.

    2016-09-01

    In the last decade, Adaptive Optics has been used to compensate the aberrations of the eye in order to acquire high resolution retinal images. The use of high speed deformable mirrors (DMs) to accomplish this compensation in real time is of great importance. But, sometimes DMs are overused, compensating the aberrations inherent in the optical systems. In this work the evaluation of the performance of an adaptive optics system together with the imaging system will be evaluated in order to know in advance the aberrations inherent in them in order to compensate them prior the use of a DM.

  3. Real-Time X-Ray Inspection

    NASA Technical Reports Server (NTRS)

    Bulthuis, Ronald V.

    1988-01-01

    X-ray imaging instrument adapted to continuous scanning. Modern version of fluoroscope enables rapid x-ray inspection of parts. Developed for detection of buckling in insulated ducts. Uses radiation from radioactive gadolinium or thallium source. Instrument weighs only 6 1/2 lb. Quickly scanned by hand along duct surface, providing real-time image. Based on Lixiscope, developed at Goddard Space Flight Center.

  4. Further Studies on Nonlinear Adaptive Optics,

    DTIC Science & Technology

    1981-04-01

    AD-A9 167 SCIENCE APPLICATIONS INC LA JOLLA CA F/9 20/6 A-A*9 16 FURTHER STUDIES ON NONLINEAR ADAPTIVE OPTICS , 1W _ ASFE APR SI A ELCI. J1 NAGEL. D...FURTHER STUDIES ON NONLINEAR ADAPTIVE OPTICS Apr 8l 7 Submitted to: Director of Physics Air Force Office of Scientific Research ATTN: NP Bldg. 410...1 I STATEMENT OF WORK ...... .. .................... I-I II NONLINEAR ADAPTIVE OPTICS SUMMARY

  5. Self-tuning control algorithm design for vehicle adaptive cruise control system through real-time estimation of vehicle parameters and road grade

    NASA Astrophysics Data System (ADS)

    Marzbanrad, Javad; Tahbaz-zadeh Moghaddam, Iman

    2016-09-01

    The main purpose of this paper is to design a self-tuning control algorithm for an adaptive cruise control (ACC) system that can adapt its behaviour to variations of vehicle dynamics and uncertain road grade. To this aim, short-time linear quadratic form (STLQF) estimation technique is developed so as to track simultaneously the trend of the time-varying parameters of vehicle longitudinal dynamics with a small delay. These parameters are vehicle mass, road grade and aerodynamic drag-area coefficient. Next, the values of estimated parameters are used to tune the throttle and brake control inputs and to regulate the throttle/brake switching logic that governs the throttle and brake switching. The performance of the designed STLQF-based self-tuning control (STLQF-STC) algorithm for ACC system is compared with the conventional method based on fixed control structure regarding the speed/distance tracking control modes. Simulation results show that the proposed control algorithm improves the performance of throttle and brake controllers, providing more comfort while travelling, enhancing driving safety and giving a satisfactory performance in the presence of different payloads and road grade variations.

  6. Embedded Electro-Optic Sensor Network for the On-Site Calibration and Real-Time Performance Monitoring of Large-Scale Phased Arrays

    DTIC Science & Technology

    2005-07-09

    This final report summarizes the progress during the Phase I SBIR project entitled "Embedded Electro - Optic Sensor Network for the On-Site Calibration...sensor network based on an electro - optic field-detection technique (the Electro - optic Sensor Network, or ESN) for the performance evaluation of phased

  7. Reversal of the lattice structure in SrCoO(x) epitaxial thin films studied by real-time optical spectroscopy and first-principles calculations.

    PubMed

    Choi, Woo Seok; Jeen, Hyoungjeen; Lee, Jun Hee; Seo, S S Ambrose; Cooper, Valentino R; Rabe, Karin M; Lee, Ho Nyung

    2013-08-30

    Using real-time spectroscopic ellipsometry, we directly observed a reversible lattice and electronic structure evolution in SrCoO(x) (x=2.5-3) epitaxial thin films. Drastically different electronic ground states, which are extremely susceptible to the oxygen content x, are found in the two topotactic phases: i.e., the brownmillerite SrCoO2.5 and the perovskite SrCoO3. First-principles calculations confirmed substantial differences in the electronic structure, including a metal-insulator transition, which originate from the modification in the Co valence states and crystallographic structures. More interestingly, the two phases can be reversibly controlled by changing the ambient pressure at greatly reduced temperatures. Our finding provides an important pathway to understanding the novel oxygen-content-dependent phase transition uniquely found in multivalent transition metal oxides.

  8. OPAD-EDIFIS Real-Time Processing

    NASA Technical Reports Server (NTRS)

    Katsinis, Constantine

    1997-01-01

    The Optical Plume Anomaly Detection (OPAD) detects engine hardware degradation of flight vehicles through identification and quantification of elemental species found in the plume by analyzing the plume emission spectra in a real-time mode. Real-time performance of OPAD relies on extensive software which must report metal amounts in the plume faster than once every 0.5 sec. OPAD software previously written by NASA scientists performed most necessary functions at speeds which were far below what is needed for real-time operation. The research presented in this report improved the execution speed of the software by optimizing the code without changing the algorithms and converting it into a parallelized form which is executed in a shared-memory multiprocessor system. The resulting code was subjected to extensive timing analysis. The report also provides suggestions for further performance improvement by (1) identifying areas of algorithm optimization, (2) recommending commercially available multiprocessor architectures and operating systems to support real-time execution and (3) presenting an initial study of fault-tolerance requirements.

  9. Real-time optoacoustic monitoring during thermotherapy

    NASA Astrophysics Data System (ADS)

    Esenaliev, Rinat O.; Larina, Irina V.; Larin, Kirill V.; Motamedi, Massoud

    2000-05-01

    Optoacoustic monitoring of tissue optical properties and speed of sound in real time can provide fast and accurate feedback information during thermotherapy performed with various heating or cooling agents. Amplitude and temporal characteristics of optoacoustic pressure waves are dependent on tissue properties. Detection and measurement of the optoacoustic waves may be used to monitor the extent of tissue hyperthermia, coagulation, or freezing with high resolution and contrast. We studied real-time optoacoustic monitoring of thermal coagulation induced by conductive heating and laser radiation and cryoablation with liquid nitrogen. Q-switched Nd:YAG laser pulses were used as probing radiation to induce optoacoustic waves in tissues. Dramatic changes in optoacoustic signal parameters were detected during tissue freezing and coagulation due to sharp changes in tissue properties. The dimensions of thermally- induced lesions were measured in real time with the optoacoustic technique. Our studies demonstrated that the laser optoacoustic technique is capable of real-time monitoring of tissue coagulation and freezing front with submillimeter spatial resolution. This may allow accurate thermal ablation or cryotherapy of malignant and benign lesions with minimal damage to normal tissues.

  10. Real-time preprocessing of holographic information

    NASA Astrophysics Data System (ADS)

    Schilling, Bradley W.; Poon, Ting-Chung

    1995-11-01

    Optical scanning holography (OSH) is a holographic recording technique that uses active optical heterodyne scanning to generate holographic information pertaining to an object. The holographic information manifests itself as an electrical signal suitable for real-time image reconstruction using a spatial light modulator. The electrical signal that carries the holographic information can also be digitized for computer storage and processing, allowing the image reconstruction to be performed numerically. In previous experiments with this technique, holographic information has been recorded using the interference pattern of a plane wave and a spherical wave of different temporal frequencies to scan an object. However, the proper manipulation of the pupil functions in the recording stage can result in real-time processing of the holographic edge extraction technique as an important example of real-time preprocessing of holographic information that utilizes alternate pupils in the OSH recording stage. We investigate the theory of holographic preprocessing using a spatial frequency-domain analysis based on the recording system's optical transfer function. The theory is reinforced through computer simulation.

  11. An adaptive optics imaging system designed for clinical use.

    PubMed

    Zhang, Jie; Yang, Qiang; Saito, Kenichi; Nozato, Koji; Williams, David R; Rossi, Ethan A

    2015-06-01

    Here we demonstrate a new imaging system that addresses several major problems limiting the clinical utility of conventional adaptive optics scanning light ophthalmoscopy (AOSLO), including its small field of view (FOV), reliance on patient fixation for targeting imaging, and substantial post-processing time. We previously showed an efficient image based eye tracking method for real-time optical stabilization and image registration in AOSLO. However, in patients with poor fixation, eye motion causes the FOV to drift substantially, causing this approach to fail. We solve that problem here by tracking eye motion at multiple spatial scales simultaneously by optically and electronically integrating a wide FOV SLO (WFSLO) with an AOSLO. This multi-scale approach, implemented with fast tip/tilt mirrors, has a large stabilization range of ± 5.6°. Our method consists of three stages implemented in parallel: 1) coarse optical stabilization driven by a WFSLO image, 2) fine optical stabilization driven by an AOSLO image, and 3) sub-pixel digital registration of the AOSLO image. We evaluated system performance in normal eyes and diseased eyes with poor fixation. Residual image motion with incremental compensation after each stage was: 1) ~2-3 arc minutes, (arcmin) 2) ~0.5-0.8 arcmin and, 3) ~0.05-0.07 arcmin, for normal eyes. Performance in eyes with poor fixation was: 1) ~3-5 arcmin, 2) ~0.7-1.1 arcmin and 3) ~0.07-0.14 arcmin. We demonstrate that this system is capable of reducing image motion by a factor of ~400, on average. This new optical design provides additional benefits for clinical imaging, including a steering subsystem for AOSLO that can be guided by the WFSLO to target specific regions of interest such as retinal pathology and real-time averaging of registered images to eliminate image post-processing.

  12. An adaptive optics imaging system designed for clinical use

    PubMed Central

    Zhang, Jie; Yang, Qiang; Saito, Kenichi; Nozato, Koji; Williams, David R.; Rossi, Ethan A.

    2015-01-01

    Here we demonstrate a new imaging system that addresses several major problems limiting the clinical utility of conventional adaptive optics scanning light ophthalmoscopy (AOSLO), including its small field of view (FOV), reliance on patient fixation for targeting imaging, and substantial post-processing time. We previously showed an efficient image based eye tracking method for real-time optical stabilization and image registration in AOSLO. However, in patients with poor fixation, eye motion causes the FOV to drift substantially, causing this approach to fail. We solve that problem here by tracking eye motion at multiple spatial scales simultaneously by optically and electronically integrating a wide FOV SLO (WFSLO) with an AOSLO. This multi-scale approach, implemented with fast tip/tilt mirrors, has a large stabilization range of ± 5.6°. Our method consists of three stages implemented in parallel: 1) coarse optical stabilization driven by a WFSLO image, 2) fine optical stabilization driven by an AOSLO image, and 3) sub-pixel digital registration of the AOSLO image. We evaluated system performance in normal eyes and diseased eyes with poor fixation. Residual image motion with incremental compensation after each stage was: 1) ~2–3 arc minutes, (arcmin) 2) ~0.5–0.8 arcmin and, 3) ~0.05–0.07 arcmin, for normal eyes. Performance in eyes with poor fixation was: 1) ~3–5 arcmin, 2) ~0.7–1.1 arcmin and 3) ~0.07–0.14 arcmin. We demonstrate that this system is capable of reducing image motion by a factor of ~400, on average. This new optical design provides additional benefits for clinical imaging, including a steering subsystem for AOSLO that can be guided by the WFSLO to target specific regions of interest such as retinal pathology and real-time averaging of registered images to eliminate image post-processing. PMID:26114033

  13. HEVC real-time decoding

    NASA Astrophysics Data System (ADS)

    Bross, Benjamin; Alvarez-Mesa, Mauricio; George, Valeri; Chi, Chi Ching; Mayer, Tobias; Juurlink, Ben; Schierl, Thomas

    2013-09-01

    The n