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Sample records for adjacent control sites

  1. Colposcopy of vaginal and vulvar human papillomavirus and adjacent sites.

    PubMed

    Hatch, K

    1993-03-01

    Human papillomaviral infections can affect the entire lower female genital tract as multifocal or multicentric disease as well as the surrounding anatomic and adjacent sites. The traditional colposcopic methods are necessary to assist in the diagnosis and help differentiate these infections from other disease mimics. PMID:8392676

  2. 1. OVERVIEW SHOWING FIRING CONTROL BLOCKHOUSE 0502 AND ADJACENT OBSERVATION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. OVERVIEW SHOWING FIRING CONTROL BLOCKHOUSE 0502 AND ADJACENT OBSERVATION TOWER. WATER BRAKE TROUGH SEGMENT AT LOWER RIGHT. Looking north northeast. - Edwards Air Force Base, South Base Sled Track, Firing & Control Blockhouse for 10,000-foot Track, South of Sled Track at midpoint of 20,000-foot track, Lancaster, Los Angeles County, CA

  3. 49 CFR 236.404 - Signals at adjacent control points.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Signals at adjacent control points. 236.404 Section 236.404 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RULES, STANDARDS, AND INSTRUCTIONS GOVERNING THE INSTALLATION, INSPECTION, MAINTENANCE, AND REPAIR...

  4. 7. WALKWAY/ENTRANCE TO ADMINSITRATIVE SITE ADJACENT TO ENTRANCE ROAD AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. WALKWAY/ENTRANCE TO ADMINSITRATIVE SITE ADJACENT TO ENTRANCE ROAD AND INTERNAL POLICE POST, LOOKING SOUTHEAST - Manzanar War Relocation Center, Owens Valley off U.S. Highway 395, 6 miles South of Independence, Independence, Inyo County, CA

  5. Coordinated tissue-specific regulation of adjacent alternative 3′ splice sites in C. elegans

    PubMed Central

    Ragle, James Matthew; Katzman, Sol; Akers, Taylor F.; Barberan-Soler, Sergio; Zahler, Alan M.

    2015-01-01

    Adjacent alternative 3′ splice sites, those separated by ≤18 nucleotides, provide a unique problem in the study of alternative splicing regulation; there is overlap of the cis-elements that define the adjacent sites. Identification of the intron's 3′ end depends upon sequence elements that define the branchpoint, polypyrimidine tract, and terminal AG dinucleotide. Starting with RNA-seq data from germline-enriched and somatic cell-enriched Caenorhabditis elegans samples, we identify hundreds of introns with adjacent alternative 3′ splice sites. We identify 203 events that undergo tissue-specific alternative splicing. For these, the regulation is monodirectional, with somatic cells preferring to splice at the distal 3′ splice site (furthest from the 5′ end of the intron) and germline cells showing a distinct shift toward usage of the adjacent proximal 3′ splice site (closer to the 5′ end of the intron). Splicing patterns in somatic cells follow C. elegans consensus rules of 3′ splice site definition; a short stretch of pyrimidines preceding an AG dinucleotide. Splicing in germline cells occurs at proximal 3′ splice sites that lack a preceding polypyrimidine tract, and in three instances the germline-specific site lacks the AG dinucleotide. We provide evidence that use of germline-specific proximal 3′ splice sites is conserved across Caenorhabditis species. We propose that there are differences between germline and somatic cells in the way that the basal splicing machinery functions to determine the intron terminus. PMID:25922281

  6. 15 CFR Appendix D to Subpart M of... - Dredged Material Disposal Sites Adjacent to the Monterey Bay National Marine Sanctuary

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... disposal site adjacent to the Sanctuary off of the Golden Gate: Point ID No. Latitude Longitude 1 37.76458... 15 Commerce and Foreign Trade 3 2010-01-01 2010-01-01 false Dredged Material Disposal Sites... Subpart M of Part 922—Dredged Material Disposal Sites Adjacent to the Monterey Bay National...

  7. 15 CFR Appendix D to Subpart M of... - Dredged Material Disposal Sites Adjacent to the Monterey Bay National Marine Sanctuary

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... disposal site adjacent to the Sanctuary off of the Golden Gate: Point ID No. Latitude Longitude 1 37.76458... 15 Commerce and Foreign Trade 3 2011-01-01 2011-01-01 false Dredged Material Disposal Sites... Subpart M of Part 922—Dredged Material Disposal Sites Adjacent to the Monterey Bay National...

  8. Using a triad approach in the assessment of hazardous waste site leaching from a Superfund site to an adjacent stream

    SciTech Connect

    Leppanen, C.J.; Blanner, P.M.; Allan, R.S.; Maier, K.J.; Benson, W.H.

    1998-10-01

    A triad approach was used in the evaluation of sediment in the Wolf River adjacent to the North Hollywood Dump, a federally listed Superfund site. Chemical analyses were done for 18 organochlorine pesticides, 21 polychlorinated biphenyl (PCB) congeners, and 10 metals. Sediment toxicity was evaluated with freshwater invertebrates. Chironomus tentans and Hyalella azteca. Benthic macroinvertebrate diversity and abundance were assessed with a family-level biotic index. Mean Al, Ba, Cd, Cu, Fe, Ni, Pb, and Zn concentrations were significantly higher in sediments collected in the spring. Both spring and fall sediments exhibited toxicity downstream from, adjacent to, and upstream from the dump, with toxicity significantly higher in fall sediments; however, a consistent trend was not observed. Toxicity was typically greater in the fall, and metal concentrations were typically higher in spring sediments, suggesting that metals were not responsible for the toxicity. Sediment-associated organochlorine pesticide and PCB congener concentrations were all below detectable limits, suggesting that these potential contaminants are not contributing to the observed toxicity. No differences were found in benthic macroinvertebrate community structure, which was composed of predominantly pollution-tolerant families, among seasons or river reaches, which appear to be limited by the physical characteristics of the river. Sediments in urban reaches of the Wolf River appear to be degraded; the North Hollywood Dump cannot be isolated as a source of toxicity in this study. In situ testing, sediment toxicity identification and evaluation testing, acid-volatile sulfide analyses, or artificial substrate work would be appropriate to follow.

  9. DNA sequence templates adjacent nucleosome and ORC sites at gene amplification origins in Drosophila

    PubMed Central

    Liu, Jun; Zimmer, Kurt; Rusch, Douglas B.; Paranjape, Neha; Podicheti, Ram; Tang, Haixu; Calvi, Brian R.

    2015-01-01

    Eukaryotic origins of DNA replication are bound by the origin recognition complex (ORC), which scaffolds assembly of a pre-replicative complex (pre-RC) that is then activated to initiate replication. Both pre-RC assembly and activation are strongly influenced by developmental changes to the epigenome, but molecular mechanisms remain incompletely defined. We have been examining the activation of origins responsible for developmental gene amplification in Drosophila. At a specific time in oogenesis, somatic follicle cells transition from genomic replication to a locus-specific replication from six amplicon origins. Previous evidence indicated that these amplicon origins are activated by nucleosome acetylation, but how this affects origin chromatin is unknown. Here, we examine nucleosome position in follicle cells using micrococcal nuclease digestion with Ilumina sequencing. The results indicate that ORC binding sites and other essential origin sequences are nucleosome-depleted regions (NDRs). Nucleosome position at the amplicons was highly similar among developmental stages during which ORC is or is not bound, indicating that being an NDR is not sufficient to specify ORC binding. Importantly, the data suggest that nucleosomes and ORC have opposite preferences for DNA sequence and structure. We propose that nucleosome hyperacetylation promotes pre-RC assembly onto adjacent DNA sequences that are disfavored by nucleosomes but favored by ORC. PMID:26227968

  10. Evaluation of fall chinook salmon spawning adjacent to the In-Situ Redox Manipulation treatability test site, Hanford Site, Washington

    SciTech Connect

    Mueller, R.P.; Geist, D.R.

    1998-10-02

    The In Situ Redox Manipulation (ISRM) experiment is being evaluated as a potential method to remove contaminants from groundwater adjacent to the Columbia River near the 100-D Area. The ISRM experiment involves using sodium dithionate (Na{sub 2}O{sub 6}S{sub 2}) to precipitate chromate from the groundwater. The treatment will likely create anoxic conditions in the groundwater down-gradient of the ISRM treatability test site; however, the spatial extent of this anoxic plume is not exactly known. Surveys were conducted in November 1997, following the peak spawning of fall chinook salmon. Aerial surveys documented 210 redds (spawning nests) near the downstream island in locations consistent with previous surveys. Neither aerial nor underwater surveys documented fall chinook spawning in the vicinity of the ISRM treatability test site. Based on measurements of depth, velocity, and substrate, less than 1% of the study area contained suitable fall chinook salmon spawning habitat, indicating low potential for fall chinook salmon to spawn in the vicinity of the ISRM experiment.

  11. Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome

    PubMed Central

    Shi, Yuan; Chen, Xiang; Elsasser, Suzanne; Stocks, Bradley B.; Tian, Geng; Lee, Byung-Hoon; Shi, Yanhong; Zhang, Naixia; de Poot, Stefanie A. H.; Tuebing, Fabian; Sun, Shuangwu; Vannoy, Jacob; Tarasov, Sergey G.; Engen, John R.; Finley, Daniel; Walters, Kylie J.

    2016-01-01

    , multipoint binding of ubiquitin chains. The structures of the T1 site in its free state and complexed with monoubiquitin or K48-linked diubiquitin were solved, revealing that three neighboring outer helices from the T1 toroid engage two ubiquitins. This binding mode leads to a preference for certain ubiquitin chain types, especially K6- and K48-linked chains, in a distinct configuration that can position substrates close to the entry port of the proteasome. The fate of proteasome-docked ubiquitin conjugates is determined by a competition between deubiquitination and substrate degradation. We find that proximal to the T1 site within the Rpn1 toroid is a second UBL-binding site, T2, that does not assist in ubiquitin chain recognition, but rather in chain disassembly, by binding to the UBL domain of deubiquitinating enzyme Ubp6. Importantly, the UBL interactors at T1 and T2 are distinct, assigning substrate localization to T1 and substrate deubiquitination to T2. CONCLUSION A ligand-binding hotspot was identified in the Rpn1 toroid, consisting of two adjacent receptor sites, T1 and T2. The Rpn1 toroid represents a novel class of binding domains for ubiquitin and UBL proteins. This study thus defines a novel two-site recognition domain intrinsic to the proteasome that uses homologous ubiquitin/UBL-class ligands to assemble substrates, substrate shuttling factors, and a deubiquitinating enzyme in close proximity. A ligand-binding hotspot in the proteasome for assembling substrates and cofactors Schematic (top) and model structure (bottom, left) mapping the UBL-binding Rpn1 T1 (indigo) and T2 (orange) sites. (Bottom, right) Enlarged region of the proteasome to illustrate the Rpn1 T1 and T2 sites bound to a ubiquitin chain (yellow) and deubiquitinating enzyme Ubp6 (green), respectively. PDB 4CR2 and 2B9R were used for this figure. Hundreds of pathways for degradation converge at ubiquitin recognition by proteasome. Here we found that the five known proteasomal ubiquitin receptors

  12. 15 CFR Appendix D to Subpart M of... - Dredged Material Disposal Sites Adjacent to the Monterey Bay National Marine Sanctuary

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 3 2014-01-01 2014-01-01 false Dredged Material Disposal Sites Adjacent to the Monterey Bay National Marine Sanctuary D Appendix D to Subpart M of Part 922 Commerce and... SANCTUARY PROGRAM REGULATIONS Monterey Bay National Marine Sanctuary Pt. 922, Subpt. M, App. D Appendix D...

  13. Assessment of the hydrologic interaction between Imikpuk Lake and the adjacent airstrip site near Barrow, Alaska, 1993

    USGS Publications Warehouse

    McCarthy, Kathleen A.; Solin, Gary L.; Trabant, Dennis

    1994-01-01

    Imikpuk Lake serves as the drinking water source for the Ukpeagvik Inupiat Corporation-National Arctic Research Laboratory (UIC-NARL), formerly known as the Naval Arctic Research Laboratory, near Barrow, Alaska. During the 1970's and 1980's, accidental releases of more than 1,300 cubic meters of various types of fuel occurred at the airstrip site adjacent to the lake. To aid an assessment of the potential risk 10 the quality of water in the lake posed by fuel remaining in the subsurface, the hydrologic interaction between the lake and ground water at the airstrip site was examined. The study area lies within the region of continuous permafrost where hydrologic processes are largely controlled by the short annual thaw season and the presence of near-surface permafrost. Runoff occurs for only a short period each year, typically from early or mid-June to late September, and a shallow ground- water system develops during approximately the same period as a result of shallow thawing of the subsurface. During the spring and summer of 1993, snowpack and surface-water data were collected throughout the Imikpuk Lake basin, and subsurface- flow-system data were collected at the airstrip site. The total annual inflow to the lake was estimated 10 be approximately 300,000 cubic meters per year, based on four methods of estimation. The ground-water flow system at the airstrip site is complex, primarily because of variations in local land-surface topography. Subsurface frost-elevation data indicate that a permafrost ridge exists beneath one of the elevated building pads at the site. Similar ridges beneath elevated roadways at the site may act as impediments to ground-water flow, reducing the flux of subsurface water to Imikpuk Lake. However, on the basis of the assumption that such impediments do not reduce flux substantially, the ground-water flux from the airstrip site was estimated to be approximately 173 cubic meters per year--less than 0.1 percent of the estimated annual

  14. Shaking table experimentation on adjacent structures controlled by passive and semi-active MR dampers

    NASA Astrophysics Data System (ADS)

    Basili, M.; De Angelis, M.; Fraraccio, G.

    2013-06-01

    This paper presents the results of shaking table tests on adjacent structures controlled by passive and semi-active MR dampers. The aim was to demonstrate experimentally the effectiveness of passive and semi-active strategies in reducing structural vibrations due to seismic excitation. The physical model at issue was represented by two adjacent steel structures, respectively of 4 and 2 levels, connected at the second level by a MR damper. When the device operated in semi-active mode, an ON-OFF control algorithm, derived by the Lyapunov stability theory, was implemented and experimentally validated. Since the experimentation concerned adjacent structures, two control objectives have been reached: global and selective protection. In case of global protection, the attention was focused on protecting both structures, whereas, in case of selective protection, the attention was focused on protecting only one structure. For each objective the effectiveness of passive control has been compared with the situation of no control and then the effectiveness of semi-active control has been compared with the passive one. The quantities directly compared have been: measured displacements, accelerations and force-displacement of the MR damper, moreover some global response quantities have been estimated from experimental measures, which are the base share force and the base bending moment, the input energy and the energy dissipated by the device. In order to evaluate the effectiveness of the control action in both passive and semi-active case, an energy index EDI, previously defined and already often applied numerically, has been utilized. The aspects investigated in the experimentation have been: the implementation and validation of the control algorithm for selective and global protection, the MR damper input voltage influence, the kind of seismic input and its intensity.

  15. Hybrid control approach for seismic coupling of two similar adjacent structures

    NASA Astrophysics Data System (ADS)

    Park, Kwan-Soon; Ok, Seung-Yong

    2015-08-01

    This study describes an optimal hybrid control approach of two similar adjacent buildings for seismic performance improvement, for which the passive dampers are used as link members between the two parallel buildings and the active control devices are installed as tendon-type devices between two successive floors in the buildings. Throughout this configuration, the passive coupling dampers modulate the relative responses between the two buildings and the active control devices modulate the inter-story responses of each building. In order to achieve global optimal control performance, genetic algorithm is employed to perform an integrated design approach for the system of buildings and hybrid control devices. Through the optimization process, the passive and active devices are optimally distributed along the floors and, simultaneously, their damping capacities and active controllers are configured in accordance with the distribution. The proposed approach is demonstrated in comparison with typical active control system which consists of two independent active systems without any connections between two buildings, each being optimal for one particular building. The numerical simulation results for a system of 20-story and 16-story buildings reveal that the proposed system can guarantee the competent control performance over the typical independent active system with less control forces and powers. As a result, it is proved that the proposed approach enables the simultaneous optimization of the control performance and the control cost.

  16. Zone of Interaction Between Hanford Site Groundwater and Adjacent Columbia River

    SciTech Connect

    Peterson, Robert E.; Connelly, Michael P.

    2001-10-23

    This report describes the FY 2000 results of a Science and Technology investigation of the groundwater/river interface at the Hanford Site. The investigation focused on (1) a 2-D simulation of water flowpaths beneath the shoreline region under the influence of a transient river stage, and (2) mixing between groundwater and river water.

  17. Study of Heavy Metals in a Wetland Area Adjacent to a Waste Disposal Site Near Resolute Bay, Canadian High Arctic

    NASA Astrophysics Data System (ADS)

    Lund, K. E.; Young, K. L.

    2004-05-01

    Heavy metal contamination in High Arctic systems is of growing concern. Studies have been conducted measuring long range and large point source pollutants, but little research has been done on small point sources such as municipal waste disposal sites. Many Arctic communities are coastal, and local people consume marine wildlife in which concentrations of heavy metals can accumulate. Waste disposal sites are often located in very close proximity to the coastline and leaching of these metals could contaminate food sources on a local scale. Cadmium and lead are the metals focussed on by this study, as the Northern Contaminants Program recognizes them as metals of concern. During the summer of 2003 a study was conducted near Resolute, Nunavut, Canada, to determine the extent of cadmium and lead leaching from a local dumpsite to an adjacent wetland. The ultimate fate of these contaminants is approximately 1 km downslope in the ocean. Transects covering an area of 0.3 km2 were established downslope from the point of disposal and water and soil samples were collected and analyzed for cadmium and lead. Only trace amounts of cadmium and lead were found in the water samples. In the soil samples, low uniform concentrations of cadmium were found that were slightly above background levels, except for adjacent to the point of waste input where higher concentrations were found. Lead soil concentrations were higher than cadmium and varied spatially with soil material and moisture. Overall, excessive amounts of cadmium and lead contamination do not appear to be entering the marine ecosystem. However, soil material and moisture should be considered when establishing waste disposal sites in the far north

  18. Late summer heat-transfer regimes at adjacent permafrost and non-permafrost sites in central Alaska

    SciTech Connect

    Hinkel, K.M.; Nicholas, J. . Dept. of Geography); Outcalt, S.I. . Dept. of Geological Sciences)

    1992-01-01

    Hourly observations of soil temperature and a surrogate index of soil water ion concentration were collected during late summer from the upper 50 cm of soil at two adjacent sites in the discontinuous permafrost zone of central Alaska. One site is above permafrost while the other, 13 m away in an area of weak groundwater discharge, has no underlying permafrost. At the permafrost site, temperatures at the surface of the dry, porous organic mat experienced large diurnal variation but temperature amplitude was strongly attenuated with depth. Steep thermal gradients induce diffusion of water vapor from the base of the active layer toward the surface. Evaporative cooling thus inhibits heat penetration and maintains subfreezing temperatures at depth. However, infiltration of precipitation is an effective method of transporting sensible heat to the base of the active layer and extending seasonal thaw depth above permafrost. Conversely, at the groundwater seep site, saturation maintained by throughflow damps temperature variation with time and depth, and precipitation has little impact on the evolution of the thermal regime. Soil conditions and precipitation patterns strongly influence the nonconductive heat flux component and produce complex spatial and temporal thaw patterns.

  19. Distinct roles of beta1 metal ion-dependent adhesion site (MIDAS), adjacent to MIDAS (ADMIDAS), and ligand-associated metal-binding site (LIMBS) cation-binding sites in ligand recognition by integrin alpha2beta1.

    PubMed

    Valdramidou, Dimitra; Humphries, Martin J; Mould, A Paul

    2008-11-21

    Integrin-ligand interactions are regulated in a complex manner by divalent cations, and previous studies have identified ligand-competent, stimulatory, and inhibitory cation-binding sites. In collagen-binding integrins, such as alpha2beta1, ligand recognition takes place exclusively at the alpha subunit I domain. However, activation of the alphaI domain depends on its interaction with a structurally similar domain in the beta subunit known as the I-like or betaI domain. The top face of the betaI domain contains three cation-binding sites: the metal-ion dependent adhesion site (MIDAS), the ADMIDAS (adjacent to MIDAS), and LIMBS (ligand-associated metal-binding site). The role of these sites in controlling ligand binding to the alphaI domain has yet to be elucidated. Mutation of the MIDAS or LIMBS completely blocked collagen binding to alpha2beta1; in contrast mutation of the ADMIDAS reduced ligand recognition but this effect could be overcome by the activating monoclonal antibody TS2/16. Hence, the MIDAS and LIMBS appear to be essential for the interaction between alphaI and betaI, whereas occupancy of the ADMIDAS has an allosteric effect on the conformation of betaI. An activating mutation in the alpha2 I domain partially restored ligand binding to the MIDAS and LIMBS mutants. Analysis of the effects of Ca(2+), Mg(2+), and Mn(2+) on ligand binding to these mutants showed that the MIDAS is a ligand-competent site through which Mn(2+) stimulates ligand binding, whereas the LIMBS is a stimulatory Ca(2+)-binding site, occupancy of which increases the affinity of Mg(2+) for the MIDAS. PMID:18820259

  20. Self-Association Is Required for Occupation of Adjacent Binding Sites in Pseudomonas aeruginosa Type III Secretion System Promoters

    PubMed Central

    Marsden, Anne E.; Schubot, Florian D.

    2014-01-01

    ExsA is a member of the AraC/XylS family of transcriptional regulators and is required for expression of the Pseudomonas aeruginosa type III secretion system (T3SS). All P. aeruginosa T3SS promoters contain two adjacent binding sites for monomeric ExsA. The amino-terminal domain of ExsA (NTD) is thought to mediate interactions between the ExsA monomers bound to each site. Threading the NTD onto the AraC backbone revealed an α-helix that likely serves as the primary determinant for dimerization. In this study, we performed alanine scanning mutagenesis of the ExsA α-helix (residues 136 to 152) to identify determinants required for self-association. Residues L137, C139, L140, K141, and L148 exhibited self-association defects and were required for maximal activation by ExsA. Disruption of self-association resulted in decreased binding to T3SS promoters, particularly loss of binding by the second ExsA monomer. Removing the NTD or increasing the space between the ExsA-binding sites restored the ability of the second ExsA monomer to bind the PexsC promoter. This finding indicated that, in the absence of self-association, the NTD prevents binding by a second monomer. Similar findings were seen with the PexoT promoter; however, binding of the second ExsA monomer in the absence of self-association also required the presence of a high-affinity site 2. Based on these data, ExsA self-association is necessary to overcome inhibition by the NTD and to compensate for low-affinity binding sites, thereby allowing for full occupation and activation of ExsA-dependent promoters. Therefore, ExsA self-association is indispensable and provides an attractive target for antivirulence therapies. PMID:25070741

  1. Environmental Factors Controlling Ostracod Distribution on the Tibetan Plateau and Adjacent Areas

    NASA Astrophysics Data System (ADS)

    Boerner, N.; Pröttel, R.; Glodniok, S.; Söndgerath, D.; Naumann, K.; Frenzel, P.; Mischke, S.; Schwalb, A.

    2014-12-01

    Non-marine ostracods represent a reliable tool to reconstruct past climate conditions. The investigation of environmental tolerances of modern ostracod species is therefore vital to estimate past climate conditions based on fossil assemblages. To determine the climate range of individual ostracod species their modern geographical distribution is combined with climate data sets. The overlap of modern ecological tolerances of ostracod species found in a fossil assemblage yield information about past air temperatures [1]. In our study we establish ecological tolerances of species from the Tibetan Plateau and adjacent areas and indicate which environmental parameter is most important for species distribution. Therefore the point biserial correlation coefficient is applied on the species distribution of 34 taxa from 364 locations based on the presence/absence of species. We use a 0.93 km gridded climate database that pairs climate parameters with modern collection sites. In total we investigated the influence of 20 different environmental parameters, such as annual temperature amplitude, July and January temperatures, precipitation, salinity and altitude. The yearly temperature range is the most important factor defining the distribution for Candona candida and Fabaeformiscandona gyirongensis. The distribution of Eucypris mareotica is also influenced by the yearly temperature range, but in addition is restricted by low salinities. Species showing no correlation to temperature variations in their distribution were also identified. The distribution of Limnocythere inopinata and Heterocypris salina is restricted by the amount of precipitation, especially during winter months, and low salinities. On the other hand, Candona neglectaseems to be unaffected by any of the studied parameters and probably only is limited by extremely high salinities. The calculated mutual climate ranges of the investigated ostracod species will be applied to ostracod assemblage data from a long

  2. Site-specific impacts on gene expression and behavior in fathead minnows (Pimephales promelas) exposed in situ to streams adjacent to sewage treatment plants

    PubMed Central

    2009-01-01

    Background Environmental monitoring for pharmaceuticals and endocrine disruptors in the aquatic environment traditionally employs a variety of methods including analytical chemistry, as well as a variety of histological and biochemical endpoints that correlate with the fish fitness. It is now clear that analytical chemistry alone is insufficient to identify aquatic environments that are compromised because these measurements do not identify the biologically available dose. The biological endpoints that are measured are important because they relate to known impairments; however, they are not specific to the contaminants and often focus on only a few known endpoints. These studies can be enhanced by looking more broadly at changes in gene expression, especially if the analysis focuses on biochemical pathways. The present study was designed to obtain additional information for well-characterized sites adjacent to sewage treatment plants in MN that are thought to be impacted by endocrine disruptors. Results Here we examine five sites that have been previously characterized and examine changes in gene expression in fathead minnows (Pimephales promelas) that have been caged for 48 h in each of the aquatic environments. We find that the gene expression changes are characteristic and unique at each of the five sites. Also, fish exposed to two of the sites, 7 and 12, present a more aggressive behavior compared to control fish. Conclusion Our results show that a short-term exposure to sewage treatment plant effluents was able to induce a site-specific gene expression pattern in the fathead minnow gonad and liver. The short-term exposure was also enough to affect fish sexual behavior. Our results also show that microarray analysis can be very useful at determining potential exposure to chemicals, and could be used routinely as a tool for environmental monitoring. PMID:19811676

  3. The North Slope of Alaska and Adjacent Arctic Ocean (NSA/AAO) cart site begins operation: Collaboration with SHEBA and FIRE

    SciTech Connect

    Zak, D. B.; Church, H.; Ivey, M.; Yellowhorse, L.; Zirzow, J.; Widener, K. B.; Rhodes, P.; Turney, C.; Koontz, A.; Stamnes, K.; Storvold, R.; Eide, H. A.; Utley, P.; Eagan, R.; Cook, D.; Hart, D.; Wesely, M.

    2000-04-04

    Since the 1997 Atmospheric Radiation Measurement (ARM) Science Team Meeting, the North Slope of Alaska and Adjacent Arctic Ocean (NSA/AAO) Cloud and Radiation Testbed (CART) site has come into being. Much has happened even since the 1998 Science Team Meeting at which this paper was presented. To maximize its usefulness, this paper has been updated to include developments through July 1998.

  4. Correspondence of ectomycorrhizal diversity and colonisation of willows (Salix spp.) grown in short rotation coppice on arable sites and adjacent natural stands.

    PubMed

    Hrynkiewicz, Katarzyna; Toljander, Ylva K; Baum, Christel; Fransson, Petra M A; Taylor, Andy F S; Weih, Martin

    2012-11-01

    Willows (Salix spp.) are mycorrhizal tree species sometimes cultivated as short rotation coppice (SRC) on arable sites for energy purposes; they are also among the earliest plants colonising primary successional sites in natural stands. The objective of this study was to analyse the degree of colonisation and diversity of ectomycorrhizal (EM) communities on willows grown as SRC in arable soils and their adjacent natural or naturalized stands. Arable sites usually lack ectomycorrhizal host plants before the establishment of SRC, and adjacent natural or naturalized willow stands were hypothesized to be a leading source of ectomycorrhizal inoculum for the SRC. Three test sites including SRC stands (Salix viminalis, Salix dasyclados, and Salix schwerinii) and adjacent natural or naturalized (Salix caprea, Salix fragilis, and Salix × mollissima) stands in central Sweden were investigated on EM colonisation and morphotypes, and the fungal partners of 36 of the total 49 EM fungi morphotypes were identified using molecular tools. The frequency of mycorrhizas in the natural/naturalized stands was higher (two sites) or lower (one site) than in the corresponding cultivated stands. Correspondence analysis revealed that some EM taxa (e.g. Agaricales) were mostly associated with cultivated willows, while others (e.g. Thelephorales) were mostly found in natural/naturalized stands. In conclusion, we found strong effects of sites and willow genotype on EM fungi formation, but poor correspondence between the EM fungi abundance and diversity in SRC and their adjacent natural/naturalized stands. The underlying mechanism might be selective promotion of some EM fungi species by more effective spore dispersal. PMID:22415721

  5. Evaluation of the physical process controlling beach changes adjacent to nearshore dredge pits

    USGS Publications Warehouse

    Benedet, L.; List, J.H.

    2008-01-01

    Numerical modeling of a beach nourishment project is conducted to enable a detailed evaluation of the processes associated with the effects of nearshore dredge pits on nourishment evolution and formation of erosion hot spots. A process-based numerical model, Delft3D, is used for this purpose. The analysis is based on the modification of existing bathymetry to simulate "what if" scenarios with/without the bathymetric features of interest. Borrow pits dredged about 30??years ago to provide sand for the nourishment project have a significant influence on project performance and formation of erosional hot spots. It was found that the main processes controlling beach response to these offshore bathymetric features were feedbacks between wave forces (roller force or alongshore component of the radiation stress), pressure gradients due to differentials in wave set-up/set-down and bed shear stress. Modeling results also indicated that backfilling of selected borrow sites showed a net positive effect within the beach fill limits and caused a reduction in the magnitude of hot spot erosion. ?? 2008 Elsevier B.V. All rights reserved.

  6. 24 CFR 583.320 - Site control.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 3 2014-04-01 2013-04-01 true Site control. 583.320 Section 583... DEVELOPMENT COMMUNITY FACILITIES SUPPORTIVE HOUSING PROGRAM Program Requirements § 583.320 Site control. (a) Site control. (1) Where grant funds will be used for acquisition, rehabilitation, or new...

  7. 24 CFR 583.320 - Site control.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 3 2013-04-01 2013-04-01 false Site control. 583.320 Section 583... DEVELOPMENT COMMUNITY FACILITIES SUPPORTIVE HOUSING PROGRAM Program Requirements § 583.320 Site control. (a) Site control. (1) Where grant funds will be used for acquisition, rehabilitation, or new...

  8. 24 CFR 583.320 - Site control.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 3 2012-04-01 2012-04-01 false Site control. 583.320 Section 583... DEVELOPMENT COMMUNITY FACILITIES SUPPORTIVE HOUSING PROGRAM Program Requirements § 583.320 Site control. (a) Site control. (1) Where grant funds will be used for acquisition, rehabilitation, or new...

  9. 24 CFR 583.320 - Site control.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 3 2010-04-01 2010-04-01 false Site control. 583.320 Section 583... DEVELOPMENT COMMUNITY FACILITIES SUPPORTIVE HOUSING PROGRAM Program Requirements § 583.320 Site control. (a) Site control. (1) Where grant funds will be used for acquisition, rehabilitation, or new...

  10. 24 CFR 583.320 - Site control.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 3 2011-04-01 2010-04-01 true Site control. 583.320 Section 583... DEVELOPMENT COMMUNITY FACILITIES SUPPORTIVE HOUSING PROGRAM Program Requirements § 583.320 Site control. (a) Site control. (1) Where grant funds will be used for acquisition, rehabilitation, or new...

  11. Radioecology of Vertebrate Animals in the Area Adjacent to the Chernobyl Nuclear Power Plant Site in 1986-2008

    NASA Astrophysics Data System (ADS)

    Farfan, E. B.; Gashchak, S. P.; Makliuk, Y. A.; Maksymenko, A. M.; Bondarkov, M. D.; Jannik, G. T.; Marra, J. C.

    2009-12-01

    A widespread environmental contamination of the areas adjacent to the Chernobyl Nuclear Power Plant (ChNPP) site attracted a great deal of publicity to the biological consequences of the ChNPP catastrophe. However, only a few studies focused on a detailed analysis of radioactive contamination of the local wild fauna and most of them were published in Eastern European languages, making them poorly accessible for Western scientists. In addition, evaluation of this information appears difficult due to significant differences in raw data acquisition and analysis methodologies and final data presentation formats. Using an integrated approach to assessment of all available information, the International Radioecology Laboratory scientists showed that the ChNPP accident had increased the average values of the animals 137Cs and 90Sr contamination by a factor of thousands, followed by its decrease by a factor of tens, primarily resulting from a decrease in the biological accessibility of the radionuclides. However, this trend depended on many factors. Plant and bottom feeding fish species were the first to reach the maximum contamination levels. No data are available on other vertebrates, but it can be assumed that the same trend was true for all plant feeding animals and animals searching for food on the soil surface. The most significant decrease of the average values occurred during the first 3-5 years after the accident and it was the most pronounced for elks and plant and plankton feeding fish. Their diet included elements “alienated” from the major radionuclide inventory; for example, upper soil layers and bottom deposits where the fallout that had originally precipitated on plants, water and soils gradually migrated. Further radionuclide penetration into deeper layers of soils and its bonding with their mineral components intensified decontamination of the fauna. It took a while for the contamination of predatory fish and mammals (wolves) to reach the maximum

  12. Controls on bacterial gas accumulations in thick Tertiary coal beds and adjacent channel sandstones, Powder River basin, Wyoming and Montana

    SciTech Connect

    Rice, D.D.; Flores, R.M. )

    1991-03-01

    Coal beds, as much as 250 ft thick, and adjacent sandstones in the Paleocene Tongue River Member of the Fort Union Formation are reservoirs for coal-derived natural gas in the Powder River basin. The discontinuous coal beds were deposited in raised, ombrotrophic peat bogs about 3 mi{sup 2} in size, adjoining networks of fluvial channels infilled by sand. Coal-bed thickness was controlled by basin subsidence and depositional environments. The average maceral composition of the coals is 88% huminite (vitrinite), 5% liptinite, and 7% inertinite. The coals vary in rank from subbituminous C to A (R{sub o} values of 0.4 to 0.5%). Although the coals are relatively low rank, they display fracture systems. Natural gas desorbed and produced from the coal beds and adjacent sandstones is composed mainly of methane with lesser amount of Co{sub 2} ({lt}10%). The methane is isotopically light and enriched in deuterium. The gases are interpreted to be generated by bacterial processes and the fermentation pathway, prior to the main phase of thermogenic methane generation by devolatilization. Large amounts of bicarbonate water generated during early stages of coalification will have to be removed from the fracture porosity in the coal beds before desorption and commercial gas production can take place. Desorbed amounts of methane-rich, bacterial gas in the Powder River basin are relatively low ({lt}60 Scf/ton) compared to amounts of thermogenic coal-bed gases (hundreds of Scf/ton) from other Rocky Mountain basins. However, the total coal-bed gas resource in both the coal beds and the adjacent sandstones is considered to be large (as much as 40 Tcf) because of the vast coal resources (as much as 1.3 trillion tons).

  13. Spatial distribution and controlling factors of sedimentary bodies in Jiaozhou Bay and Adjacent Sea Areas, Qingdao

    NASA Astrophysics Data System (ADS)

    Dong, Heping; Li, Guangxue; Li, Shuanglin; Li, Shaoquan; Li, Chun

    2011-06-01

    The distributions of thickness of unconsolidated Quaternary sedimentary layers in Jiaozhou Bay and Qingdao offshore area were studied by using 1079-km high-resolution shallow seismic profiles and drilling core data, and the factors controlling the Quaternary evolution were discussed. The results show that such thickness distributions resulted from the coactions of geologic structures and marine hydrodynamic conditions since the Holocene. The geologic structures controlled the slope deposit, proluvial and fluvial fillings since the late Pleistocene. Holocene marine hydrodynamics eroded away sediments at the bay mouth, and tides carried these eroded materials to the sides of the bay mouth and released them there, forming channel-ridge-alternating geomorphic features. During transgressive processes, the sea level rose rapidly, and insufficient sediment supply and tidal actions yielded the relict sediments in the east of Qingdao offshore area.

  14. A Pit-1 Binding Site Adjacent to E-box133 in the Rat PRL Promoter is Necessary for Pulsatile Gene Expression Activity.

    PubMed

    Bose, Sudeep; Ganguly, Surajit; Kumar, Sachin; Boockfor, Fredric R

    2016-06-01

    Recent evidence reveals that prolactin gene expression (PRL-GE) in mammotropes occurs in pulses, but the molecular process(es) underlying this phenomenon remains unclear. Earlier, we have identified an E-box (E-box133) in the rat PRL promoter that binds several circadian elements and is critical for this dynamic process. Preliminary analysis revealed a Pit-1 binding site (P2) located immediately adjacent to this E-box133 raising the possibility that some type of functional relationship may exist between these two promoter regions. In this study, using serum shocked GH3 cell culture system to synchronize PRL-GE activity, we determined that Pit-1 gene expression occurred in pulses with time phases similar to that for PRL. Interestingly, EMSA analysis not only confirmed Pit-1 binding to the P2 site, but also revealed an interaction with factor(s) binding to the adjacent E-box133 promoter element. Additionally, down-regulation of Pit-1 by siRNA reduced PRL levels during pulse periods. Thus, using multiple evidences, our results demonstrate clearly that the Pit-1 P2 site is necessary for PRL-GE elaboration. Furthermore, the proximity of this critical Pit-1 binding site (P2) and the E-box133 element coupled with the evidences of a site-to-site protein interactions suggest that the process of PRL-GE pulse activity might involve more dynamic and intricate cross-talks between promoter elements that may span some, or all, of the proximal region of the PRL promoter in driving its pulsatile expression. PMID:26875730

  15. HIGH BREAST MILK LEVELS OF POLYCHLORINATEDE BIPHENYLS (PCBS) AMONG FOUR WOMEN LIVING ADJACENT TO A PCB-CONTAMINATED WASTE SITE

    EPA Science Inventory

    As a consequence of contamination by effluents from local electronics manufacturing facilities, the New Bedford Harbor and estuary in southeastern Massachusetts is among the sites in the United States that are considered the most highly contaminated by polychlorinated biphenyls (...

  16. Electrical control of Co/Ni magnetism adjacent to gate oxides with low oxygen ion mobility

    SciTech Connect

    Yan, Y. N.; Zhou, X. J.; Li, F.; Cui, B.; Wang, Y. Y.; Wang, G. Y.; Pan, F.; Song, C.

    2015-09-21

    We investigate the electrical manipulation of Co/Ni magnetization through a combination of ionic liquid and oxide gating, where HfO{sub 2} with a low O{sup 2−} ion mobility is employed. A limited oxidation-reduction process at the metal/HfO{sub 2} interface can be induced by large electric field, which can greatly affect the saturated magnetization and Curie temperature of Co/Ni bilayer. Besides the oxidation/reduction process, first-principles calculations show that the variation of d electrons is also responsible for the magnetization variation. Our work discloses the role of gate oxides with a relatively low O{sup 2−} ion mobility in electrical control of magnetism, and might pave the way for the magneto-ionic memory with low power consumption and high endurance performance.

  17. Charge-patterning phase transition on a surface lattice of titratable sites adjacent to an electrolyte solution

    NASA Astrophysics Data System (ADS)

    Shore, Joel; Thurston, George

    We discuss a model for a charge-patterning phase transition on a two-dimensional square lattice of titratable sites, here regarded as protonation sites, placed on a square lattice in a dielectric medium just below the planar interface between this medium and an aqueous salt solution. Within Debye-Huckel theory, the analytical form of the electrostatic repulsion between protonated sites exhibits an approximate inverse cubic power-law decrease beyond short distances. The problem can thus be mapped onto the two-dimensional antiferromagnetic Ising model with this longer-range interaction, which we study with Monte Carlo simulations. As we increase pH, the occupation probability of a site decreases from 1 at low pH to 0 at high pH. For sufficiently-strong interaction strengths, a phase transition occurs as the occupation probability of 1/2 is approached: the charges arrange themselves into a checkerboard pattern. This ordered phase persists over a range of pH until a transition occurs back to a disordered state. This state is the analogue of the Neel state in the antiferromagnetic Ising spin model. More complicated ordered phases are expected for sufficiently strong interactions (with occupation probabilities of 1/4 and 3/4) and if the lattice is triangular rather than square. This work was supported by NIH EY018249 (GMT).

  18. Changes in Nematode Communities in Different Physiographic Sites of the Condor Seamount (North-East Atlantic Ocean) and Adjacent Sediments

    PubMed Central

    Zeppilli, Daniela; Bongiorni, Lucia; Serrão Santos, Ricardo; Vanreusel, Ann

    2014-01-01

    Several seamounts are known as ‘oases’ of high abundances and biomass and hotspots of biodiversity in contrast to the surrounding deep-sea environments. Recent studies have indicated that each single seamount can exhibit a high intricate habitat turnover. Information on alpha and beta diversity of single seamount is needed in order to fully understand seamounts contribution to regional and global biodiversity. However, while most of the seamount research has been focused on summits, studies considering the whole seamount structure are still rather poor. In the present study we analysed abundance, biomass and diversity of nematodes collected in distinct physiographic sites and surrounding sediments of the Condor Seamount (Azores, North-East Atlantic Ocean). Our study revealed higher nematode biomass in the seamount bases and values 10 times higher in the Condor sediments than in the far-field site. Although biodiversity indices did not showed significant differences comparing seamount sites and far-field sites, significant differences were observed in term of nematode composition. The Condor summit harboured a completely different nematode community when compared to the other seamount sites, with a high number of exclusive species and important differences in term of nematode trophic diversity. The oceanographic conditions observed around the Condor Seamount and the associated sediment mixing, together with the high quality of food resources available in seamount base could explain the observed patterns. Our results support the hypothesis that seamounts maintain high biodiversity through heightened beta diversity and showed that not only summits but also seamount bases can support rich benthic community in terms of standing stocks and diversity. Furthermore functional diversity of nematodes strongly depends on environmental conditions link to the local setting and seamount structure. This finding should be considered in future studies on seamounts, especially in

  19. Changes in nematode communities in different physiographic sites of the condor seamount (north-East atlantic ocean) and adjacent sediments.

    PubMed

    Zeppilli, Daniela; Bongiorni, Lucia; Serrão Santos, Ricardo; Vanreusel, Ann

    2014-01-01

    Several seamounts are known as 'oases' of high abundances and biomass and hotspots of biodiversity in contrast to the surrounding deep-sea environments. Recent studies have indicated that each single seamount can exhibit a high intricate habitat turnover. Information on alpha and beta diversity of single seamount is needed in order to fully understand seamounts contribution to regional and global biodiversity. However, while most of the seamount research has been focused on summits, studies considering the whole seamount structure are still rather poor. In the present study we analysed abundance, biomass and diversity of nematodes collected in distinct physiographic sites and surrounding sediments of the Condor Seamount (Azores, North-East Atlantic Ocean). Our study revealed higher nematode biomass in the seamount bases and values 10 times higher in the Condor sediments than in the far-field site. Although biodiversity indices did not showed significant differences comparing seamount sites and far-field sites, significant differences were observed in term of nematode composition. The Condor summit harboured a completely different nematode community when compared to the other seamount sites, with a high number of exclusive species and important differences in term of nematode trophic diversity. The oceanographic conditions observed around the Condor Seamount and the associated sediment mixing, together with the high quality of food resources available in seamount base could explain the observed patterns. Our results support the hypothesis that seamounts maintain high biodiversity through heightened beta diversity and showed that not only summits but also seamount bases can support rich benthic community in terms of standing stocks and diversity. Furthermore functional diversity of nematodes strongly depends on environmental conditions link to the local setting and seamount structure. This finding should be considered in future studies on seamounts, especially in

  20. Control of White Spot Lesion Adjacent to Orthodontic Bracket with Use of Fluoride Varnish or Chlorhexidine Gel

    PubMed Central

    Restrepo, Manuel; Bussaneli, Diego G.; Jeremias, Fabiano; Cordeiro, Rita C. L.; Magalhães, Ana C.; Palomari Spolidorio, Denise M.; Santos-Pinto, Lourdes

    2015-01-01

    The aims of this study were to compare the effectiveness of fluoride varnish and chlorhexidine gel in controlling white spot lesions (WSLs) adjacent to orthodontic brackets and to compare the ability of Quantitative Light-Induced Fluorescence (QLF) to measure mineral uptake with that of transverse microradiography (TMR). Thirty premolars with artificially induced WSLs were randomly assigned to three groups: (1) two applications of 5% NaF-varnish (F), with one-week interval, (2) two applications of 2% chlorhexidine gel (CHX), with one-week interval, and (3) control (CO), no treatment. QLF was used to measure changes in fluorescence before and after caries induction, 1 week after each application and 1, 2, and 3 months after the last application of F or CHX. TMR was performed to quantify lesion depth and mineral content after caries induction to evaluate the effects of F, CHX, and CO 3 months after the last application of agents. The data were analyzed by repeated measures ANOVA and Tukey's test. All treatments increased the mineral content during the experimental period; however, F induced faster remineralization than CHX. The correlation between QLF and TMR was significantly moderate. Two applications of fluoride varnish or 2% chlorhexidine gel at one-week intervals were effective in controlling WSLs. PMID:25973442

  1. Controlling factors of summer phytoplankton community in the Changjiang (Yangtze River) Estuary and adjacent East China Sea shelf

    NASA Astrophysics Data System (ADS)

    Jiang, Zhibing; Chen, Jianfang; Zhou, Feng; Shou, Lu; Chen, Quanzhen; Tao, Bangyi; Yan, Xiaojun; Wang, Kui

    2015-06-01

    We analyzed the composition and distribution of phytoplankton in relation to physicochemical factors in the Changjiang (Yangtze River) Estuary and adjacent East China Sea shelf in June and August 2009. Diatoms and dinoflagellates dominated the community, particularly in eutrophic inshore waters controlled by the Changjiang Diluted Water (CDW), coastal current, and upwelling. However, high densities of cyanobacteria and cryptophytes were observed in the oligotrophic offshore waters influenced by the Taiwan Warm Current (TWC) and Kuroshio. In June, the northeastward CDW plume combined with the Yellow Sea Coastal Current induced algal bloom in the northern part of the CE. In August, the enhanced CDW formed two narrow, low-salinity tongues that extended eastward and southward (associated with the upwelling and coastal current), resulting in phytoplankton blooms off the CE and in the Zhejiang coastal waters, respectively. Phytoplankton abundance in August was considerably higher than in June, with increased solar radiation, CDW, and upwelling. The maximum abundance occurred on the surface in inshore turbid waters and on the subsurface (5-30 m) in offshore clear waters with increased stratification. Based on multidimensional scaling and cluster analysis, we found appreciable spatio-temporal variations in algal community composition. Different ecological groups corresponded with hydrographic distributions. Canonical correspondence analysis showed that nutrients, salinity, temperature, and suspended particulate matter were the main variables associated with community distribution. We suggest that the variations in summer phytoplankton community are highly correlated with the significant monthly and spatial variability in physicochemical properties, which are primarily controlled by the CDW and TWC.

  2. Lance water injection tests adjacent to the 281-3H retention basin at the Savannah River Site, Aiken, South Carolina

    SciTech Connect

    Freifeld, B.; Myer, L.; Moridis, G.; Cook, P.; James, A.; Pellerin, L.; Pruess, K.

    1996-09-01

    A pilot-scale field demonstration of waste isolation using viscous- liquid containment barriers has been planned for the 281-3H retention basin at the Savannah River Site, Aiken, SC. The 281-3H basin is a shallow retention/seepage basin contaminated mainly by radionuclides. The viscous-liquid containment barrier utilizes the permeation of liquid grout to either entomb the contaminants within a monolithic grout structure or to isolate the waste by drastically reducing the permeability, of the soils around the plume. A clear understanding of the hydrogeologic setting of the retention basin is necessary for proper design of the viscous liquid barrier. To aid in the understanding of the hydrogeology of the 281-3H retention basin, and to obtain critical parameters necessary for grout injection design, a series of tests were undertaken in a region immediately adjacent to the basin. The objectives of the LWIT were: 1. To evaluate the general performance of the Lance Injection Technique for grout emplacement at the site, including the range and upper limits of injection pressures, the flow rates applicable for site conditions, as well as the mechanical forces needed for lance penetration. 2. To obtain detailed information on the injectability of the soils immediately adjacent to the H-area retention basin. 3. To identify any high permeability zones suitable for injection and evaluate their spatial distribution. 4. To perform ground penetrating radar (GPR) to gain information on the structure of the soil column and to compare the results with LWIT data. This report will focus on results pertinent to these objectives.

  3. A catalog of borehole geophysics for the 100 Areas and adjacent 600 Area, Hanford Site, 1962 to May 1992

    SciTech Connect

    Lewis, R E; Pearson, A W

    1992-09-01

    This report catalogs geophysical borehole logs acquired between 1962, when logging began, and May 15, 1992 in the 100 Areas and the surrounding 600 Area of the Hanford Site. Separate tables were prepared for each respective set of wells. Each table lists all wells known to have been drilled, all borehole geophysical logs measured, and the location of these logs. No information is provided for logs acquired by the Westinghouse Hanford Company spectral gamma logging system. Maps are provided for identification of the specific wells in the 1200 Areas. A cross reference between the well numbering scheme employed by the Basalt Waste Isolation Project and that based on the Hanford grid name is provided for the appropriate wells in the 600 area.

  4. Mixed Inhibition of cPEPCK by Genistein, Using an Extended Binding Site Located Adjacent to Its Catalytic Cleft

    PubMed Central

    Dhanjal, Jaspreet Kaur; Sundar, Durai

    2015-01-01

    Cytosolic phosphoenolpyruvate carboxykinase (cPEPCK) is a critical enzyme involved in gluconeogenesis, glyceroneogenesis and cataplerosis. cPEPCK converts oxaloacetic acid (OAA) into phosphoenol pyruvate (PEP) in the presence of GTP. cPEPCK is known to be associated with type 2 diabetes. Genistein is an isoflavone compound that shows anti-diabetic and anti-obesitic properties. Experimental studies have shown a decrease in the blood glucose level in the presence of genistein by lowering the functional activity of cPEPCK, an enzyme of gluconeogenesis. Using computational techniques such as molecular modeling, molecular docking, molecular dynamics simulation and binding free energy calculations, we identified cPEPCK as a direct target of genistein. We studied the molecular interactions of genistein with three possible conformations of cPEPCK—unbound cPEPCK (u_cPEPCK), GTP bound cPEPCK (GTP_cPEPCK) and GDP bound cPEPCK (GDP_cPEPCK). Binding of genistein was also compared with an already known cPEPCK inhibitor. We analyzed the interactions of genistein with cPEPCK enzyme and compared them with its natural substrate (OAA), product (PEP) and known inhibitor (3-MPA). Our results demonstrate that genistein uses the mechanism of mixed inhibition to block the functional activity of cPEPCK and thus can serve as a potential anti-diabetic and anti-obesity drug candidate. We also identified an extended binding site in the catalytic cleft of cPEPCK which is used by 3-MPA to inhibit cPEPCK non-competitively. We demonstrate that extended binding site of cPEPCK can further be exploited for designing new drugs against cPEPCK. PMID:26528723

  5. Changes in chemical contaminant body burden and biological effects in mussels adjacent to a marine remediation site

    SciTech Connect

    Kagley, A.N.; Snider, R.G.; Inouye, L.S.; Casillas, E.

    1995-12-31

    Eagle Harbor is a creosote-polluted marine site currently undergoing initial environmental remediation. Highly contaminated sediments were capped with a layer of sediment from a minimally contaminated area. Mussels (Mytilus edulis complex) in the vicinity of the creosote plant were collected before, during, and after the initial remediation process, for monitoring body burdens of PAHs as well as cellular effects indicative of biological damage. Mussels from this area have previously been shown to exhibit an elevated body burden of high molecular weight PAHs, as well as substantial changes in subcellular structures and functions, characteristic of mussels from chemically contaminated environments. Following capping, the body burden of high molecular weight PAHs was substantially reduced early in the restoration process yet mussel contaminant body burdens were approaching pre-cap levels one year after the end of the remediation project. Changes in mussel health were assessed by measuring selected aspects of lysosomal function as well as levels of enzymes and anti-oxidants involved in detoxifying organic chemical contaminants. Substantial improvement throughout capping occurred in lysosomal stability and cytochrome P450 reductase activity in digestive gland, and anti-oxidant status in gill tissue when compared to initial findings. In contrast, increased levels of neutral lipid and lipofuscin in digestive glands indicated that mussels were still suffering biological impairment as a result of chemical contaminant exposure. Overall, indigenous mussels near the marine remediation project showed temporary improvement in tissue body burden of chemical contaminants and some decrease in biological effects.

  6. Retention by the endoplasmic reticulum of rotavirus VP7 is controlled by three adjacent amino-terminal residues.

    PubMed Central

    Maass, D R; Atkinson, P H

    1994-01-01

    The rotavirus outer capsid glycoprotein, VP7, is an endoplasmic reticulum (ER) membrane-associated glycoprotein in both infected and transfected cells. It was previously demonstrated in this laboratory and by others that both the cleaved signal sequence (H2) and the first NH2-terminal 61 amino acids of VP7 are sufficient and necessary for ER retention of this molecule. Using site-specific mutagenesis and transfection techniques, we show that residues Ile-9, Thr-10, and Gly-11 were specifically necessary for ER retention. These results further define the ER retention sequence of VP7 and demonstrate that conservative changes, apparently innocuous in only three adjacent amino acids, can lead to major solubility and compartmentalization changes. It was found that placement of the first 31 mature NH2-terminal residues of VP7, in addition to the cleaved ER translocation signal sequence, was sufficient to retain the enzymatically active chimeric alpha-amylase in the ER; this enzyme is normally secreted. Deletions of the residues Ile-9, Thr-10, and Gly-11 within the amylase chimera containing 31 VP7 amino acids resulted in secretion of enzymatically active protein. It was also observed that the residues of VP7 presented in certain chimeras were able to abolish alpha-amylase enzymatic activity. These chimeras are presumably misfolded since it was demonstrated by pulse-chase experiments that these molecules are degraded in the ER. We surmise that a favorable conformation is necessary for retention since ER retention and activity of the chimeras depend on the primary sequence context. Images PMID:8254749

  7. Nucleotide sequence composition adjacent to intronic splice sites improves splicing efficiency via its effect on pre-mRNA local folding in fungi.

    PubMed

    Zafrir, Zohar; Tuller, Tamir

    2015-10-01

    RNA splicing is the central process of intron removal in eukaryotes known to regulate various cellular functions such as growth, development, and response to external signals. The canonical sequences indicating the splicing sites needed for intronic boundary recognition are well known. However, the roles and evolution of the local folding of intronic and exonic sequence features adjacent to splice sites has yet to be thoroughly studied. Here, focusing on four fungi (Saccharomyces cerevisiae, Schizosaccharomyces pombe, Aspergillus nidulans, and Candida albicans), we performed for the first time a comprehensive high-resolution study aimed at characterizing the encoding of intronic splicing efficiency in pre-mRNA transcripts and its effect on intron evolution. Our analysis supports the conjecture that pre-mRNA local folding strength at intronic boundaries is under selective pressure, as it significantly affects splicing efficiency. Specifically, we show that in the immediate region of 12-30 nucleotides (nt) surrounding the intronic donor site there is a preference for weak pre-mRNA folding; similarly, in the region of 15-33 nt surrounding the acceptor and branch sites there is a preference for weak pre-mRNA folding. We also show that in most cases there is a preference for strong pre-mRNA folding further away from intronic splice sites. In addition, we demonstrate that these signals are not associated with gene-specific functions, and they correlate with splicing efficiency measurements (r = 0.77, P = 2.98 × 10(-21)) and with expression levels of the corresponding genes (P = 1.24 × 10(-19)). We suggest that pre-mRNA folding strength in the above-mentioned regions has a direct effect on splicing efficiency by improving the recognition of intronic boundaries. These new discoveries are contributory steps toward a broader understanding of splicing regulation and intronic/transcript evolution. PMID:26246046

  8. Snail control in urban sites in Brazil with slow-release hexabutyldistannoxane and pentachlorophenol*

    PubMed Central

    Toledo, J. V.; Da Silva, C. S. Monteiro; Bulhões, M. S.; Leme, L. A. Paes; Netto, J. A. Da Silva; Gilbert, B.

    1976-01-01

    Slow release formulations of hexabutyldistannoxane (TBTO) and pentachlorophenol (PCP) were tested for the control of Biomphalaria tenagophila in 52 urban sites in Rio de Janeiro. TBTO acted faster and lasted longer than PCP and at 15 g/m2 it eliminated snails from 76% of the treated sites for 1 year. Water pollution and rate of flow had no significant influence on the molluscicidal properties of either compound, but alkalinity lowered the activity of TBTO. Failure to control snail populations was due mainly to human interference and to the non-treatment of adjacent breeding sites that were temporarily dry and therefore overlooked. PMID:1088356

  9. Geology, hydrogeology, and potential of intrinsic bioremediation at the National Park Service Dockside II site and adjacent areas, Charleston, South Carolina, 1993-94

    USGS Publications Warehouse

    Campbell, B.G.; Petkewich, M.D.; Landmeyer, J.E.; Chapelle, F.H.

    1996-01-01

    A long history of industrial and commercial use of the National Park Service property and adjacent properties located in downtown Charleston, South Carolina, has caused extensive contamination of the shallow subsurface soils and water-table aquifer. The National Park Service property is located adjacent to a former manufactured-gas plant site, which is the major source of the contamination. Contamination of this shallow water-table aquifer is of concern because shallow ground water discharges to the Cooper River and contains contaminants, which may affect adjacent wildlife or human populations. The geology of the National Park Service property above the Ashley Formation of the Cooper Group consists of two Quaternary lithostratigraphic marine units, the Wando Formation and Holocene deposits, overlain by artificial fill. The Wando Formation overlies the Ashley Formation, a sandy calcareous clay, and consists of soft, organic clay overlain by gray sand. The Holocene deposits are composed of clayey to silty sand and soft organic-rich clay. The artificial fill, which was placed at the site to create dry land where salt marsh existed previously, is composed of sand, silt, and various scrap materials. The shallow hydrogeology of the National Park Service property overlying the Ashley Formation can be subdivided into two sandy aquifers separated by a leaky, black, organic-rich clay. The unconfined upper surficial aquifer is primarily artificial fill. The lower surficial aquifer consists of the Wando sand unit and is confined by the leaky organic-rich clay. Aquifer tests performed on the wells screened in these aquifers resulted in hydraulic conductivities from 0.1 to 10 feet per day for the upper surficial aquifer, and 16 feet per day for the lower surficial aquifer. Vertical hydraulic gradients at the site are typically low. A downward gradient from the upper surficial aquifer to the lower surficial aquifer occurs throughout most of the year. A brick-lined storm

  10. 24 CFR 578.25 - Site control.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 3 2013-04-01 2013-04-01 false Site control. 578.25 Section 578.25 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR COMMUNITY PLANNING AND DEVELOPMENT, DEPARTMENT OF HOUSING AND URBAN...

  11. 24 CFR 578.25 - Site control.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 3 2014-04-01 2013-04-01 true Site control. 578.25 Section 578.25 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR COMMUNITY PLANNING AND DEVELOPMENT, DEPARTMENT OF HOUSING AND URBAN...

  12. By-products of a former phenol manufacturing site in a small lake adjacent to a Superfund site in the Aberjona watershed.

    PubMed Central

    Wick, L Y; Gschwend, P M

    1998-01-01

    Benzene, diphenyl sulfone (DPS), para-hydroxybiphenyl (PPP), ortho-hydroxybiphenyl (OPP), higher hydroxybiphenyls, and alkylated benzenes were found in a small lake receiving contaminated groundwater discharge from the Industri-Plex Superfund site (Woburn, MA) in the Aberjona watershed in eastern Massachusetts. All of these chemicals may derive from the former phenol manufacturing activities present at the Industri-Plex site during World War I. Concentrations up to 1660 microgram/l benzene, 450 micro/l DPS, 230 microgram/l PPP, and 100 microgram/l OPP were detected in the hypolimnion. Epilimnetic concentrations of the chemicals were significantly lower (normally < 5 microgram/l). DPS showed a distinct seasonal behavior: It was readily biodegradable during warm periods. No biodegradation was observed in the winter, leaving export to the Aberjona River as the major removal mechanism. Although benzene is known to be toxic and a human carcinogen, our results indicate that DPS, OPP, and PPP are not mutagenic in tests using human MCL-5 and h1A1v2 cell lines. Images Figure 1 PMID:9703495

  13. Evaluation of geophysical logs and video surveys in boreholes adjacent to the Berkley Products Superfund Site, West Cocalico Township, Lancaster County, Pennsylvania

    USGS Publications Warehouse

    Low, Dennis J.; Conger, Randall W.

    1998-01-01

    Between February 1998 and April 1998, geophysical logs were collected in nine boreholes adjacent to the Berkley Products Superfund Site, West Cocalico Township, Lancaster County, Pa. Video surveys were conducted on four of the nine boreholes. The boreholes range in depth from 320 to 508 feet below land surface, are completed open holes, have ambient vertical flow of water, and penetrate a series of interbedded siltstone, sandstone, and conglomerate units. The purpose of collecting geophysical-log data was to help determine horizontal and vertical distribution of contaminated ground water migrating from known or suspected sources and to aid in the placement of permanent borehole packers. The primary contaminants were derived from paint waste that included pigment sludges and wash solvents. The chlorinated volatile organic compounds probably originated from the wash solvents. Caliper logs and video surveys were used to locate fractures; inflections on fluid-resistivity and fluid-temperature logs were used to locate possible water-bearing fractures. Heatpulse-flowmeter measurements were used to verify the locations of water-producing or water-receiving zones and to measure rates of flow between water-bearing fractures. Single-point-resistance and natural-gamma logs provided information on stratigraphy. After interpretation of geophysical logs, video surveys, and driller's logs, permanent multiple-packer systems were installed in each borehole to obtain depth specific water samples from one or more water-bearing fractures in each borehole.

  14. AmeriFlux US-ARc ARM Southern Great Plains control site- Lamont

    SciTech Connect

    Torn, Margaret

    2016-01-01

    This is the AmeriFlux version of the carbon flux data for the site US-ARc ARM Southern Great Plains control site- Lamont. Site Description - The ARM SGP Control site is located in the native tallgrass prairies of the USDA Grazinglands Research Laboratory near El Reno, OK. One of two adjacent 35 ha plots with identical towers, measurements at the US-ARc unburned plot are used as the experimental control. The second plot, US-Arb, was burned on 2005/03/08. Measurement comparisons between the control and burn plot are used to address questions regarding the effects of burning activities on carbon fluxes. The region evaded burning activities for at least 15 years. Current disturbances consist of only light grazing activities.

  15. Nevada National Security Site Radiological Control Manual

    SciTech Connect

    Radiological Control Managers’ Council

    2012-03-26

    This document supersedes DOE/NV/25946--801, 'Nevada Test Site Radiological Control Manual,' Revision 1 issued in February 2010. Brief Description of Revision: A complete revision to reflect a recent change in name for the NTS; changes in name for some tenant organizations; and to update references to current DOE policies, orders, and guidance documents. Article 237.2 was deleted. Appendix 3B was updated. Article 411.2 was modified. Article 422 was re-written to reflect the wording of DOE O 458.1. Article 431.6.d was modified. The glossary was updated. This manual contains the radiological control requirements to be used for all radiological activities conducted by programs under the purview of the U.S. Department of Energy (DOE) and the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office (NNSA/NSO). Compliance with these requirements will ensure compliance with Title 10 Code of Federal Regulations (CFR) Part 835, 'Occupational Radiation Protection.' Programs covered by this manual are located at the Nevada National Security Site (NNSS); Nellis Air Force Base and North Las Vegas, Nevada; Santa Barbara and Livermore, California; and Andrews Air Force Base, Maryland. In addition, fieldwork by NNSA/NSO at other locations is covered by this manual. Current activities at NNSS include operating low-level radioactive and mixed waste disposal facilities for United States defense-generated waste, assembly and execution of subcritical experiments, assembly/disassembly of special experiments, the storage and use of special nuclear materials, performing criticality experiments, emergency responder training, surface cleanup and site characterization of contaminated land areas, environmental activity by the University system, and nonnuclear test operations, such as controlled spills of hazardous materials at the Hazardous Materials Spill Center. Currently, the major potential for occupational radiation exposure is associated with the burial of

  16. A novel phosphorylation site at Ser130 adjacent to the pseudosubstrate domain contributes to the activation of protein kinase C-δ.

    PubMed

    Gong, Jianli; Holewinski, Ronald J; Van Eyk, Jennifer E; Steinberg, Susan F

    2016-02-01

    Protein kinase C-δ (PKCδ) is a signalling kinase that regulates many cellular responses. Although most studies focus on allosteric mechanisms that activate PKCδ at membranes, PKCδ also is controlled via multi-site phosphorylation [Gong et al. (2015) Mol. Cell. Biol. 35: , 1727-1740]. The present study uses MS-based methods to identify PKCδ phosphorylation at Thr(50) and Ser(645) (in resting and PMA-treated cardiomyocytes) as well as Thr(37), Thr(38), Ser(130), Thr(164), Thr(211), Thr(215), Ser(218), Thr(295), Ser(299) and Thr(656) (as sites that increase with PMA). We focused on the consequences of phosphorylation at Ser(130) and Thr(141) (sites just N-terminal to the pseudosubstrate domain). We show that S130D and T141E substitutions co-operate to increase PKCδ's basal lipid-independent activity and that Ser(130)/Thr(141) di-phosphorylation influences PKCδ's substrate specificity. We recently reported that PKCδ preferentially phosphorylates substrates with a phosphoacceptor serine residue and that this is due to constitutive phosphorylation at Ser(357), an ATP-positioning G-loop site that limits PKCδ's threonine kinase activity [Gong et al. (2015) Mol. Cell. Biol. 35: , 1727-1740]. The present study shows that S130D and T141E substitutions increase PKCδ's threonine kinase activity indirectly by decreasing G loop phosphorylation at Ser(357). A S130F substitution [that mimics a S130F single-nt polymorphism (SNP) identified in some human populations] also increases PKCδ's maximal lipid-dependent catalytic activity and confers threonine kinase activity. Finally, we show that Ser(130)/Thr(141) phosphorylations relieve auto-inhibitory constraints that limit PKCδ's activity and substrate specificity in a cell-based context. Since phosphorylation sites map to similar positions relative to the pseudosubstrate domains of other PKCs, our results suggest that phosphorylation in this region of the enzyme may constitute a general mechanism to control PKC isoform

  17. A novel phosphorylation site at Ser130 adjacent to the pseudosubstrate domain contributes to the activation of protein kinase C-δ

    PubMed Central

    Gong, Jianli; Holewinski, Ronald J.; Van Eyk, Jennifer E.; Steinberg, Susan F.

    2016-01-01

    Protein kinase C-δ (PKCδ) is a signalling kinase that regulates many cellular responses. Although most studies focus on allosteric mechanisms that activate PKCδ at membranes, PKCδ also is controlled via multi-site phosphorylation [Gong et al. (2015) Mol. Cell. Biol. 35, 1727–1740]. The present study uses MS-based methods to identify PKCδ phosphorylation at Thr50 and Ser645 (in resting and PMA-treated cardiomyocytes) as well as Thr37, Thr38, Ser130, Thr164, Thr211, Thr215, Ser218, Thr295, Ser299 and Thr656 (as sites that increase with PMA). We focused on the consequences of phosphorylation at Ser130 and Thr141 (sites just N-terminal to the pseudosubstrate domain).We show that S130D and T141E substitutions co-operate to increase PKCδ’s basal lipid-independent activity and that Ser130/Thr141 di-phosphorylation influences PKCδ’s substrate specificity. We recently reported that PKCδ preferentially phosphorylates substrates with a phosphoacceptor serine residue and that this is due to constitutive phosphorylation at Ser357, an ATP-positioning G-loop site that limits PKCδ’s threonine kinase activity [Gong et al. (2015) Mol. Cell. Biol. 35, 1727–1740]. The present study shows that S130D and T141E substitutions increase PKCδ’s threonine kinase activity indirectly by decreasing G loop phosphorylation at Ser357. A S130F substitution [that mimics a S130F single-nt polymorphism (SNP) identified in some human populations] also increases PKCδ’s maximal lipid-dependent catalytic activity and confers threonine kinase activity. Finally, we show that Ser130/Thr141 phosphorylations relieve auto-inhibitory constraints that limit PKCδ’s activity and substrate specificity in a cell-based context. Since phosphorylation sites map to similar positions relative to the pseudosubstrate domains of other PKCs, our results suggest that phosphorylation in this region of the enzyme may constitute a general mechanism to control PKC isoform activity. PMID:26546672

  18. 4. Southwest face of missile site control building Stanley ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Southwest face of missile site control building - Stanley R. Mickelsen Safeguard Complex, Missile Site Control Building, Northeast of Tactical Road; southeast of Tactical Road South, Nekoma, Cavalier County, ND

  19. 9. East oblique of missile site control building Stanley ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. East oblique of missile site control building - Stanley R. Mickelsen Safeguard Complex, Missile Site Control Building, Northeast of Tactical Road; southeast of Tactical Road South, Nekoma, Cavalier County, ND

  20. 8. North oblique of missile site control building Stanley ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. North oblique of missile site control building - Stanley R. Mickelsen Safeguard Complex, Missile Site Control Building, Northeast of Tactical Road; southeast of Tactical Road South, Nekoma, Cavalier County, ND

  1. 3. Southeast face of missile site control building Stanley ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Southeast face of missile site control building - Stanley R. Mickelsen Safeguard Complex, Missile Site Control Building, Northeast of Tactical Road; southeast of Tactical Road South, Nekoma, Cavalier County, ND

  2. Conceptual design report for site drainage control

    SciTech Connect

    Hunter, M.R.

    1996-07-01

    The Mound Plant (Mound), located in Miamisburg, Ohio, is a Department of Energy (DOE) development and production facility performing support work for DOE`s weapons and energy-related programs. EG&G Mound Applied Technologies, Inc. (EG&G) is the Operating Contractor (OC) for this Government-Owned, Contractor-Operated (GOCO) facility. The work performed at Mound emphasizes nuclear energy and explosives technology. Mound is currently implementing an Environmental, Safety & Health (ES&H) Upgrades Program designed to protect its employees, the public, and the environment from adverse effects caused by facility activities. The first project of this multiphase program is now in the final stages of construction, and the second project is currently under design. Four additional projects, one of which is presented in this report, are in the conceptual design stage. At Mound, 22 soil zones have become contaminated with radioactive material. These zones cover approximately 20 percent of the total area of developed property at the site. During a storm event, the rainwater washes contaminated soil from these zones into the storm sewer system. These radioactive contaminants may then be discharged along with the stormwater into the Great Miami River via the Miami Erie Canal. This conceptual design report (CDR), Site Drainage Control, the fourth project in the ES&H program, describes a project that will provide improvements and much needed repairs to inadequate and deteriorating portions of the storm drainage system on the developed property. The project also will provide a stormwater retention facility capable of storing the stormwater runoff, from the developed property, resulting from a 100-year storm event. These improvements will permit the effective control and monitoring of stormwater to prevent the spread of radioactive contaminants from contaminated soil zones and will provide a means to collect and contain accidental spills of hazardous substances.

  3. 38 CFR 61.17 - Site control for capital grants.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2011-07-01 2011-07-01 false Site control for capital... (CONTINUED) VA HOMELESS PROVIDERS GRANT AND PER DIEM PROGRAM § 61.17 Site control for capital grants. (a) As... applicant must demonstrate site control through a deed, a capital lease, or an executed contract of...

  4. A tectogenetic mechanism controlling the evolution of the Texel-IJsselmeer High (northern Netherlands) and adjacent basins

    SciTech Connect

    Rijkers, R.; Geluk, M. )

    1993-09-01

    Geological studies around the Texel-IJsselmeer High have been carried out for the regional subsurface mapping project of the Geological Survey of The Netherlands. The Texel-IJsselmeer High, in the northern part of the Netherlands, is a northwest-southeast-trending structural unit, slightly tilted to the northeast. The geological evolution of the Texel-IJsselmeer High and the adjacent areas can be linked to an extensional tectonic regime during which several Jurassic basins in the Netherlands originated. During the Late Jurassic, the southern border of the Texel-IJsselmeer High was characterized by normal faulting. Main faults are dipping southwest and are generally part of a half-graben structure. Faulting is accompanied by subsidence of the hanging wall (Jurassic basin area), while the footwall (the Texel-IJsselmeer High) is isostatically uplifted and eroded. The proposed model is based on thinning of the lower crust beneath the basins during Jurassic extension by pure shear. This mechanism is coupled locally with shear zones (simple shear) as a result of lower crustal failure. The model is supported by observations on deep regional seismics at the southern margin of the basin area. During the Late Cretaceous/early Tertiary, transpressional intraplate stresses reactivated the structural weakness zones in the lower and upper crust in a reversed way (inversion). During this tectonic inversion the northwest-southeast-trending Texel-IJsselmeer High acted as a buffer zone perpendicular to the direction of maximum principal stress. Paleogeographical studies and geohistory analysis support the proposed tectogenetic model of the Texel-IJsselmeer High.

  5. Subsurface-controlled geological maps for the Y-12 plant and adjacent areas of Bear Creek Valley

    SciTech Connect

    King, H.L.; Haase, C.S.

    1987-04-01

    Bear Creek Valley in the vicinity of the US Department of Energy Y-12 Plant is underlain by Middle to Late Cambrian strata of the Conasauga Group. The group consists of interbedded limestones, shales, mudstones, and siltstones, and it can be divided into six discrete formations. Bear Creek Valley is bordered on the north by Pine Ridge, which is underlain by sandstones, siltstones, and shales of the Rome Formation, and on the south by Chestnut Ridge, which is underlain by dolostones of the Knox Group. Subsurface-controlled geological maps illustrating stratigraphic data and formational contacts for the formations within the Conasauga Group have been prepared for the Y-12 Plant vicinity and selected areas in Bear Creek Valley westward from the plant. The maps are consistent with all available surface and subsurface data for areas where sufficient data exist to make map construction feasible. 13 refs.

  6. Cellular pathways controlling integron cassette site folding.

    PubMed

    Loot, Céline; Bikard, David; Rachlin, Anna; Mazel, Didier

    2010-08-01

    By mobilizing small DNA units, integrons have a major function in the dissemination of antibiotic resistance among bacteria. The acquisition of gene cassettes occurs by recombination between the attI and attC sites catalysed by the IntI1 integron integrase. These recombination reactions use an unconventional mechanism involving a folded single-stranded attC site. We show that cellular bacterial processes delivering ssDNA, such as conjugation and replication, favour proper folding of the attC site. By developing a very sensitive in vivo assay, we also provide evidence that attC sites can recombine as cruciform structures by extrusion from double-stranded DNA. Moreover, we show an influence of DNA superhelicity on attC site extrusion in vitro and in vivo. We show that the proper folding of the attC site depends on both the propensity to form non-recombinogenic structures and the length of their variable terminal structures. These results draw the network of cell processes that regulate integron recombination. PMID:20628355

  7. Adjacent segment disease.

    PubMed

    Virk, Sohrab S; Niedermeier, Steven; Yu, Elizabeth; Khan, Safdar N

    2014-08-01

    EDUCATIONAL OBJECTIVES As a result of reading this article, physicians should be able to: 1. Understand the forces that predispose adjacent cervical segments to degeneration. 2. Understand the challenges of radiographic evaluation in the diagnosis of cervical and lumbar adjacent segment disease. 3. Describe the changes in biomechanical forces applied to adjacent segments of lumbar vertebrae with fusion. 4. Know the risk factors for adjacent segment disease in spinal fusion. Adjacent segment disease (ASD) is a broad term encompassing many complications of spinal fusion, including listhesis, instability, herniated nucleus pulposus, stenosis, hypertrophic facet arthritis, scoliosis, and vertebral compression fracture. The area of the cervical spine where most fusions occur (C3-C7) is adjacent to a highly mobile upper cervical region, and this contributes to the biomechanical stress put on the adjacent cervical segments postfusion. Studies have shown that after fusion surgery, there is increased load on adjacent segments. Definitive treatment of ASD is a topic of continuing research, but in general, treatment choices are dictated by patient age and degree of debilitation. Investigators have also studied the risk factors associated with spinal fusion that may predispose certain patients to ASD postfusion, and these data are invaluable for properly counseling patients considering spinal fusion surgery. Biomechanical studies have confirmed the added stress on adjacent segments in the cervical and lumbar spine. The diagnosis of cervical ASD is complicated given the imprecise correlation of radiographic and clinical findings. Although radiological and clinical diagnoses do not always correlate, radiographs and clinical examination dictate how a patient with prolonged pain is treated. Options for both cervical and lumbar spine ASD include fusion and/or decompression. Current studies are encouraging regarding the adoption of arthroplasty in spinal surgery, but more long

  8. Development and maintenance of the Hanford Site Radiological Control Manual

    SciTech Connect

    Munson, L.H.; Selby, J.M; Vargo, G.J.; Clark, D.L.

    1993-04-01

    In June 1992 the US Department of Energy (DOE) issued DOE N5480.6, Radiological Control, which set forth DOE's Radiological Control Program and established the framework for its implementation at sites nationwide. Accompanying the Order was the DOE Radiological Control Manual (DOE RCM), which provided the detailed requirements for the program. The Order also mandated Field Office issuance of site-specific radiological control manuals by December 1, 1992. This paper presents the approach taken to develop, review, approve, implement, and subsequently maintain the site-specific manual for the DOE Richland Field Office (RL) at Hanford Site.

  9. Development and maintenance of the Hanford Site Radiological Control Manual

    SciTech Connect

    Munson, L.H.; Selby, J.M; Vargo, G.J.; Clark, D.L.

    1993-04-01

    In June 1992 the US Department of Energy (DOE) issued DOE N5480.6, Radiological Control, which set forth DOE`s Radiological Control Program and established the framework for its implementation at sites nationwide. Accompanying the Order was the DOE Radiological Control Manual (DOE RCM), which provided the detailed requirements for the program. The Order also mandated Field Office issuance of site-specific radiological control manuals by December 1, 1992. This paper presents the approach taken to develop, review, approve, implement, and subsequently maintain the site-specific manual for the DOE Richland Field Office (RL) at Hanford Site.

  10. Phosphorylation of Simian Cytomegalovirus Assembly Protein Precursor (pAPNG.5) and Proteinase Precursor (pAPNG1): Multiple Attachment Sites Identified, Including Two Adjacent Serines in a Casein Kinase II Consensus Sequence

    PubMed Central

    Plafker, Scott M.; Woods, Amina S.; Gibson, Wade

    1999-01-01

    The assembly protein precursor (pAP) of cytomegalovirus (CMV), and its homologs in other herpesviruses, functions at several key steps during the process of capsid formation. This protein, and the genetically related maturational proteinase, is distinguished from the other capsid proteins by posttranslational modifications, including phosphorylation. The objective of this study was to identify sites at which pAP is phosphorylated so that the functional significance of this modification and the enzyme(s) responsible for it can be determined. In the work reported here, we used peptide mapping, mass spectrometry, and site-directed mutagenesis to identify two sets of pAP phosphorylation sites. One is a casein kinase II (CKII) consensus sequence that contains two adjacent serines, both of which are phosphorylated. The other site(s) is in a different domain of the protein, is phosphorylated less frequently than the CKII site, does not require preceding CKII-site phosphorylation, and causes an electrophoretic mobility shift when phosphorylated. Transfection/expression assays for proteolytic activity showed no gross effect of CKII-site phosphorylation on the enzymatic activity of the proteinase or on the substrate behavior of pAP. Evidence is presented that both the CKII sites and the secondary sites are phosphorylated in virus-infected cells and plasmid-transfected cells, indicating that these modifications can be made by a cellular enzyme(s). Apparent compartmental differences in phosphorylation of the CKII-site (cytoplasmic) and secondary-site (nuclear) serines suggest the involvement of more that one enzyme in these modifications. PMID:10516011

  11. 23. SITE BUILDING 002 SCANNER BUILDING RADAR CONTROL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. SITE BUILDING 002 - SCANNER BUILDING - RADAR CONTROL INTERFACE "RCL NO. 2" WITH COMPUTER CONTROL DISC DRIVE UNITS IN FOREGROUND. - Cape Cod Air Station, Technical Facility-Scanner Building & Power Plant, Massachusetts Military Reservation, Sandwich, Barnstable County, MA

  12. CONTROL OF AIR EMISSIONS FROM SUPERFUND SITES

    EPA Science Inventory

    This handbook is an easy-to-use tool for decision makers to evaluate emission control devices for use with Superfund remediation actions. t will assist in the selection of cost-effective control options. t is intended for use by engineers and scientists involved in preparing reme...

  13. 38 CFR 61.17 - Site control for capital grants.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Site control for capital grants. 61.17 Section 61.17 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS (CONTINUED) VA HOMELESS PROVIDERS GRANT AND PER DIEM PROGRAM § 61.17 Site control for capital grants. (a)...

  14. 2. Northwest face of missile site control building. Bottom exit ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Northwest face of missile site control building. Bottom exit is the emergency escape tunnel/unloading dock leading from the subterranean second floor at vestibule #266 and room #265 - Stanley R. Mickelsen Safeguard Complex, Missile Site Control Building, Northeast of Tactical Road; southeast of Tactical Road South, Nekoma, Cavalier County, ND

  15. 5. Distant view of west oblique of missile site control ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Distant view of west oblique of missile site control building. To right can be seen intake and exhaust of MSRPP, on far right is accessway - Stanley R. Mickelsen Safeguard Complex, Missile Site Control Building, Northeast of Tactical Road; southeast of Tactical Road South, Nekoma, Cavalier County, ND

  16. 10. East oblique of missile site control building, with better ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. East oblique of missile site control building, with better view of exhaust (the taller columns) and intake shafts - Stanley R. Mickelsen Safeguard Complex, Missile Site Control Building, Northeast of Tactical Road; southeast of Tactical Road South, Nekoma, Cavalier County, ND

  17. 13. Missile site control building, third and fourth floor interior, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Missile site control building, third and fourth floor interior, showing east corner and former electrical equipment area, room #306. This building was salvaged and sealed in the 1970's; the lower floors also suffered flooding - Stanley R. Mickelsen Safeguard Complex, Missile Site Control Building, Northeast of Tactical Road; southeast of Tactical Road South, Nekoma, Cavalier County, ND

  18. Charge-regulation phase transition on surface lattices of titratable sites adjacent to electrolyte solutions: An analog of the Ising antiferromagnet in a magnetic field

    NASA Astrophysics Data System (ADS)

    Shore, Joel D.; Thurston, George M.

    2015-12-01

    We report a charge-patterning phase transition on two-dimensional square lattices of titratable sites, here regarded as protonation sites, placed in a low-dielectric medium just below the planar interface between this medium and a salt solution. We calculate the work-of-charging matrix of the lattice with use of a linear Debye-Hückel model, as input to a grand-canonical partition function for the distribution of occupancy patterns. For a large range of parameter values, this model exhibits an approximate inverse cubic power-law decrease of the voltage produced by an individual charge, as a function of its in-lattice separation from neighboring titratable sites. Thus, the charge coupling voltage biases the local probabilities of proton binding as a function of the occupancy of sites for many neighbors beyond the nearest ones. We find that even in the presence of these longer-range interactions, the site couplings give rise to a phase transition in which the site occupancies exhibit an alternating, checkerboard pattern that is an analog of antiferromagnetic ordering. The overall strength W of this canonical charge coupling voltage, per unit charge, is a function of the Debye length, the charge depth, the Bjerrum length, and the dielectric coefficients of the medium and the solvent. The alternating occupancy transition occurs above a curve of thermodynamic critical points in the (p H-p K ,W ) plane, the curve representing a charge-regulation analog of variation of the Néel temperature of an Ising antiferromagnet as a function of an applied, uniform magnetic field. The analog of a uniform magnetic field in the antiferromagnet problem is a combination of p H-p K and W , and 1 /W is the analog of the temperature in the antiferromagnet problem. We use Monte Carlo simulations to study the occupancy patterns of the titratable sites, including interactions out to the 37th nearest-neighbor category (a distance of √{74 } lattice constants), first validating simulations

  19. Charge-regulation phase transition on surface lattices of titratable sites adjacent to electrolyte solutions: An analog of the Ising antiferromagnet in a magnetic field.

    PubMed

    Shore, Joel D; Thurston, George M

    2015-12-01

    We report a charge-patterning phase transition on two-dimensional square lattices of titratable sites, here regarded as protonation sites, placed in a low-dielectric medium just below the planar interface between this medium and a salt solution. We calculate the work-of-charging matrix of the lattice with use of a linear Debye-Hückel model, as input to a grand-canonical partition function for the distribution of occupancy patterns. For a large range of parameter values, this model exhibits an approximate inverse cubic power-law decrease of the voltage produced by an individual charge, as a function of its in-lattice separation from neighboring titratable sites. Thus, the charge coupling voltage biases the local probabilities of proton binding as a function of the occupancy of sites for many neighbors beyond the nearest ones. We find that even in the presence of these longer-range interactions, the site couplings give rise to a phase transition in which the site occupancies exhibit an alternating, checkerboard pattern that is an analog of antiferromagnetic ordering. The overall strength W of this canonical charge coupling voltage, per unit charge, is a function of the Debye length, the charge depth, the Bjerrum length, and the dielectric coefficients of the medium and the solvent. The alternating occupancy transition occurs above a curve of thermodynamic critical points in the (pH-pK,W) plane, the curve representing a charge-regulation analog of variation of the Néel temperature of an Ising antiferromagnet as a function of an applied, uniform magnetic field. The analog of a uniform magnetic field in the antiferromagnet problem is a combination of pH-pK and W, and 1/W is the analog of the temperature in the antiferromagnet problem. We use Monte Carlo simulations to study the occupancy patterns of the titratable sites, including interactions out to the 37th nearest-neighbor category (a distance of √74 lattice constants), first validating simulations through

  20. Nevada Test Site Radiological Control Manual

    SciTech Connect

    Radiological Control Managers' Council - Nevada Test Site

    2009-10-01

    This document supersedes DOE/NV/11718--079, “NV/YMP Radiological Control Manual,” Revision 5 issued in November 2004. Brief Description of Revision: A complete revision to reflect the recent changes in compliance requirements with 10 CFR 835, and for use as a reference document for Tenant Organization Radiological Protection Programs.

  1. Synthesis of supported bimetallic nanoparticles with controlled size and composition distributions for active site elucidation

    SciTech Connect

    Hakim, Sikander H.; Sener, Canan; Alba Rubio, Ana C.; Gostanian, Thomas M.; O'neill, Brandon J; Ribeiro, Fabio H.; Miller, Jeffrey T.; Dumesic, James A

    2015-08-01

    Elucidation of active sites in supported bimetallic catalysts is complicated by the high level of dispersity in the nanoparticle size and composition that is inherent in conventional methods of catalyst preparation. We present a synthesis strategy that leads to highly dispersed, bimetallic nanoparticles with uniform particle size and composition by means of controlled surface reactions. We demonstrate the synthesis of three systems, RhMo, PtMo, and RhRe, consisting of a highly reducible metal with an oxophilic promoter. These catalysts are characterized by FTIR, CO chemisorption, STEM/EDS, TPR, and XAS analysis. The catalytic properties of these bimetallic nanoparticles were probed for the selective CO hydrogenolysis of (hydroxymethyl)tetrahydropyran to produce 1,6 hexanediol. Based on the characterization results and reactivity trends, the active sites in the hydrogenolysis reaction are identified to be small ensembles of the more noble metal (Rh, Pt) adjacent to highly reduced moieties of the more oxophilic metal (Mo, Re).

  2. CHARACTERIZATION REPORT FOR STRONTIUM TITANATE IN SWSA 7 AND ADJACENT PARCELS IN SUPPORT OF THE NATIONAL PRIORITIES LIST SITE BOUNDARY DEFINITION PROGRAM OAK RIDGE, TENNESSEE

    SciTech Connect

    David A. King

    2011-10-10

    The U.S. Department of Energy (DOE) Oak Ridge Office requested support from the Oak Ridge Institute for Science and Education (ORISE) contract to delineate the extent of strontium titanate (SrTiO3) contamination in and around Solid Waste Storage Area (SWSA) 7 as part of the Oak Ridge National Priorities List Site boundary definition program. The study area is presented in Fig. 1.1 relative to the Oak Ridge Reservation (ORR). The investigation was executed according to Sampling and Analysis Plan/Quality Assurance Project Plan (SAP/QAPP) (DOE 2011) to supplement previous investigations noted below and to determine what areas, if any, have been adversely impacted by site operations.

  3. Status report on the geology of the Lawrence Livermore National Laboratory site and adjacent areas. Volume I. Text and appendices A-E

    SciTech Connect

    Carpenter, D.W.; Puchlik, K.P.; Ramirez, A.L.; Wagoner, J.L.; Knauss, K.G.; Kasameyer, P.W.

    1980-10-01

    In April, 1979, geoscience personnel at Lawrence Livermore National Laboratory (LLNL) initiated comprehensive geologic, seismologic, and hydrologic investigations of the LLNL site and nearby areas. These investigations have two objectives: 1. to obtain data for use in preparing a Final Environmental Impact Report for LLNL, pursuant to the National Environmental Policy Act; 2. to obtain data for use in improving the determination of a design basis earthquake for structural analysis of LLNL facilities. The first phases of these investigations have been completed. Work completed to date includes a comprehensive literature review, analyses of three sets of aerial photographs, reconnaissance geophysical surveys, examination of existing LLNL site borehole data, and the logging of seven exploratory trenches, segments of two sewer trenches, a deep building foundation excavation, a road cut, and an enlarged creek bank exposure. One absolute age date has been obtained by the /sup 14/C method and several dates of pedogenic carbonate formation have been obtained by the /sup 230/Th//sup 234/U method. A seismic monitoring network has been established, and planning for a site hydrologic monitoring program and strong motion instrument network has been completed. The seismologic and hydrologic investigations are beyond the scope of this report and will be discussed separately in future documents.

  4. Population genetic diversity of the northern snakehead (Channa argus) in China based on the mitochondrial DNA control region and adjacent regions sequences.

    PubMed

    Zhou, Aiguo; Zhuo, Xiaolei; Zou, Qing; Chen, Jintao; Zou, Jixing

    2015-06-01

    Genetic variation and population structure of northern snakehead (Channa argus) from eight locations in China were investigated using mitochondrial DNA control region and adjacent regions sequences. Sequence analysis showed that there were 105 haplotypes in 260 individuals, 48 unique haplotypes and 57 shared haplotypes, but no common haplotype shared by all populations. As a whole, the haplotype diversity was high (h=0.989), while the nucleotide diversity was low (π=0.00482). AMOVA analysis detected significant genetic differentiation among all eight populations (FST=0.328, p<0.01) and 66.17% of the total variance was resulted from intra-population differentiation. UPGMA analysis indicated that the eight populations could be divided into four major clusters, which was consistent with that the eight sampled locations were belonged to four isolated river systems. The neutrality and mismatch distribution tests suggested that the eight populations of C. argus in the sampling locations underwent recent population expansion. Among the eight populations, the Erhai Lake population may represent a unique genetic resource and therefore needs to be conserved. PMID:24724976

  5. Geologic and hydrologic data collected during 1976-1983 at the Sheffield low-level radioactive waste disposal site and adjacent areas, Sheffield, Illinois

    USGS Publications Warehouse

    Foster, J.B.; Garklavs, George; Mackey, G.W.

    1984-01-01

    Hydrogeologic studies were conducted at the low-level radioactive-waste disposal site near Sheffield, Illinois, from 1976-84. Data in this report include water levels in wells, lake stages, inorganic, organic, and radiometric chemical analyses of ground and surface water, hydraulic conductivities of glacial materials, grain-size distribution, clay and carbonate mineralogy, and cation exchange capacities of the glacial materials. Also included are results of petrographic analyses, physical measurements of wells, stratigraphy and lithology of cores collected from test wells, and horizontal coordinates of wells.

  6. 1. View from missile site control building (southeast to northwest) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. View from missile site control building (southeast to northwest) of universal missile building. Note earth mounding. On the far right can be seen the exit tunnel; the small "boxes" on top are the roof ventilators. This building was salvaged and sealed after site inactivation - Stanley R. Mickelsen Safeguard Complex, Universal Missile Building, Between Tactical Road South & Patrol Road, Nekoma, Cavalier County, ND

  7. Controlled trial of Biocol versus Jelonet on donor sites.

    PubMed

    Ebbehoj, J; Gavrilyuk, B K; Menzul, V A; Paklin, E L; Rochev YuA

    1996-11-01

    A controlled study of healing time in donor sites covered with Biocol, a new latex-based material, compared with donor sites covered with Jelonet showed a shorter healing time after treatment with Biocol (P < 0.001). No significant difference in cosmetic results 3 months post wounding was observed. No difference in discomfort was observed. PMID:8909760

  8. OVERALL VIEW OF THE SITE, INSTRUMENTATION AND CONTROL TANKS IN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    OVERALL VIEW OF THE SITE, INSTRUMENTATION AND CONTROL TANKS IN FOREGROUND, ROCKET TEST STAND IN BACKGROUND LEFT. - Marshall Space Flight Center, Redstone Rocket (Missile) Test Stand, Dodd Road, Huntsville, Madison County, AL

  9. 2. View from missile site control building (south to north) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. View from missile site control building (south to north) of missile launch area, showing warhead handling building in background - Stanley R. Mickelsen Safeguard Complex, Missile Launch Area, Within Exclusion Area, Nekoma, Cavalier County, ND

  10. 35. SITE BUILDING 004 ELECTRIC POWER STATION CONTROL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    35. SITE BUILDING 004 - ELECTRIC POWER STATION - CONTROL ROOM OF ELECTRIC POWER STATION WITH DIESEL ENGINE POWERED ELECTRIC GENERATION EQUIPMENT IN BACKGROUND. - Cape Cod Air Station, Technical Facility-Scanner Building & Power Plant, Massachusetts Military Reservation, Sandwich, Barnstable County, MA

  11. 22. SITE BUILDING 002 SCANNER BUILDING RADAR CONTROL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. SITE BUILDING 002 - SCANNER BUILDING - RADAR CONTROL ROOM. RECEIVER EQUIPMENT ON RIGHT WITH RF RADIATION MONITOR CABINET. - Cape Cod Air Station, Technical Facility-Scanner Building & Power Plant, Massachusetts Military Reservation, Sandwich, Barnstable County, MA

  12. SHARAD detections of subsurface reflectors near RSL sites on the Tharsis Plateau immediately adjacent to the canyon rim of Valles Marineris

    NASA Astrophysics Data System (ADS)

    Smith, I. B.; Stillman, D. E.; Phillips, R. J.; Forget, F.; Mellon, M. T.; Spiga, A.; Putzig, N. E.

    2014-12-01

    Recurring slope lineae (RSL) are very exciting features that exhibit evidence for water flow on the Martian surface. The number of RSL sites has risen to ~100 since their first detection in 2011. Those sites extend over a large portion of the Valles Marineris margin and nearby smaller canyons, but no source for this flowing water has been identified. Two possible sources exist for water near the Martian equator: the atmospheric and the subsurface. At low latitudes, atmospheric water vapor abundance is extremely low, and condensation of water from the air is unlikely. Furthermore, subsurface water ice is unlikely to remain stable in the long term, but scenarios for retaining ice on shorter timescales have not been fully tested. The Shallow Radar (SHARAD) instrument on Mars Reconnaissance Orbiter has successfully probed the subsurface of Mars to locate dielectric interfaces that delineate volcanic boundaries, layers within the polar ice caps, and ice-rock boundaries. Using the same technique of searching for dielectric contrasts at lower latitudes, we have found several detections at the highest elevations of the Tharsis Plateau, near the cliffs that form Valles Marineris at depths ranging from 30 to 80 m, depending on the dielectric properties of the overlying material. These reflectors are located near the canyon rim and slope towards the canyon, potentially crossing geologic boundaries mapped from surface data. Because of the proximity of the reflectors to RSL and the geometry of the reflections, we hypothesize that SHARAD may be detecting an ice or water reservoir that can act as a source for flowing water on the surface. We test this hypothesis by employing a one-dimensional thermal model to estimate the stability of ground ice over a wide range of durations at this latitude, including recent epochs of high obliquity, when ice would be more stable at low latitudes and for longer periods.

  13. Two Cytoplasmic Acylation Sites and an Adjacent Hydrophobic Residue, but No Other Conserved Amino Acids in the Cytoplasmic Tail of HA from Influenza A Virus Are Crucial for Virus Replication.

    PubMed

    Siche, Stefanie; Brett, Katharina; Möller, Lars; Kordyukova, Larisa V; Mintaev, Ramil R; Alexeevski, Andrei V; Veit, Michael

    2015-12-01

    Recruitment of the matrix protein M1 to the assembly site of the influenza virus is thought to be mediated by interactions with the cytoplasmic tail of hemagglutinin (HA). Based on a comprehensive sequence comparison of all sequences present in the database, we analyzed the effect of mutating conserved residues in the cytosol-facing part of the transmembrane region and cytoplasmic tail of HA (A/WSN/33 (H1N1) strain) on virus replication and morphology of virions. Removal of the two cytoplasmic acylation sites and substitution of a neighboring isoleucine by glutamine prevented rescue of infectious virions. In contrast, a conservative exchange of the same isoleucine, non-conservative exchanges of glycine and glutamine, deletion of the acylation site at the end of the transmembrane region and shifting it into the tail did not affect virus morphology and had only subtle effects on virus growth and on the incorporation of M1 and Ribo-Nucleoprotein Particles (RNPs). Thus, assuming that essential amino acids are conserved between HA subtypes we suggest that, besides the two cytoplasmic acylation sites (including adjacent hydrophobic residues), no other amino acids in the cytoplasmic tail of HA are indispensable for virus assembly and budding. PMID:26670246

  14. Two Cytoplasmic Acylation Sites and an Adjacent Hydrophobic Residue, but No Other Conserved Amino Acids in the Cytoplasmic Tail of HA from Influenza A Virus Are Crucial for Virus Replication

    PubMed Central

    Siche, Stefanie; Brett, Katharina; Möller, Lars; Kordyukova, Larisa V.; Mintaev, Ramil R.; Alexeevski, Andrei V.; Veit, Michael

    2015-01-01

    Recruitment of the matrix protein M1 to the assembly site of the influenza virus is thought to be mediated by interactions with the cytoplasmic tail of hemagglutinin (HA). Based on a comprehensive sequence comparison of all sequences present in the database, we analyzed the effect of mutating conserved residues in the cytosol-facing part of the transmembrane region and cytoplasmic tail of HA (A/WSN/33 (H1N1) strain) on virus replication and morphology of virions. Removal of the two cytoplasmic acylation sites and substitution of a neighboring isoleucine by glutamine prevented rescue of infectious virions. In contrast, a conservative exchange of the same isoleucine, non-conservative exchanges of glycine and glutamine, deletion of the acylation site at the end of the transmembrane region and shifting it into the tail did not affect virus morphology and had only subtle effects on virus growth and on the incorporation of M1 and Ribo-Nucleoprotein Particles (RNPs). Thus, assuming that essential amino acids are conserved between HA subtypes we suggest that, besides the two cytoplasmic acylation sites (including adjacent hydrophobic residues), no other amino acids in the cytoplasmic tail of HA are indispensable for virus assembly and budding. PMID:26670246

  15. Reforestation Sites Show Similar and Nested AMF Communities to an Adjacent Pristine Forest in a Tropical Mountain Area of South Ecuador

    PubMed Central

    Haug, Ingeborg; Setaro, Sabrina; Suárez, Juan Pablo

    2013-01-01

    Arbuscular mycorrhizae are important for growth and survival of tropical trees. We studied the community of arbuscular mycorrhizal fungi in a tropical mountain rain forest and in neighbouring reforestation plots in the area of Reserva Biológica San Francisco (South Ecuador). The arbuscular mycorrhizal fungi were analysed with molecular methods sequencing part of the 18 S rDNA. The sequences were classified as Operational Taxonomic Units (OTUs). We found high fungal species richness with OTUs belonging to Glomerales, Diversisporales and Archaeosporales. Despite intensive sampling, the rarefaction curves are still unsaturated for the pristine forest and the reforestation plots. The communities consisted of few frequent and many rare species. No specific interactions are recognizable. The plant individuals are associated with one to ten arbuscular mycorrhizal fungi and mostly with one to four. The fungal compositions associated with single plant individuals show a great variability and variety within one plant species. Planted and naturally occurring plants show high similarities in their fungal communities. Pristine forest and reforestation plots showed similar richness, similar diversity and a significantly nested structure of plant-AMF community. The results indicate that small-scale fragmentation presently found in this area has not destroyed the natural AMF community, at least yet. Thus, the regeneration potential of natural forest vegetation at the tested sites is not inhibited by a lack of appropriate mycobionts. PMID:23671682

  16. Reforestation sites show similar and nested AMF communities to an adjacent pristine forest in a tropical mountain area of South Ecuador.

    PubMed

    Haug, Ingeborg; Setaro, Sabrina; Suárez, Juan Pablo

    2013-01-01

    Arbuscular mycorrhizae are important for growth and survival of tropical trees. We studied the community of arbuscular mycorrhizal fungi in a tropical mountain rain forest and in neighbouring reforestation plots in the area of Reserva Biológica San Francisco (South Ecuador). The arbuscular mycorrhizal fungi were analysed with molecular methods sequencing part of the 18 S rDNA. The sequences were classified as Operational Taxonomic Units (OTUs). We found high fungal species richness with OTUs belonging to Glomerales, Diversisporales and Archaeosporales. Despite intensive sampling, the rarefaction curves are still unsaturated for the pristine forest and the reforestation plots. The communities consisted of few frequent and many rare species. No specific interactions are recognizable. The plant individuals are associated with one to ten arbuscular mycorrhizal fungi and mostly with one to four. The fungal compositions associated with single plant individuals show a great variability and variety within one plant species. Planted and naturally occurring plants show high similarities in their fungal communities. Pristine forest and reforestation plots showed similar richness, similar diversity and a significantly nested structure of plant-AMF community. The results indicate that small-scale fragmentation presently found in this area has not destroyed the natural AMF community, at least yet. Thus, the regeneration potential of natural forest vegetation at the tested sites is not inhibited by a lack of appropriate mycobionts. PMID:23671682

  17. 1. Northeast face of missile site control building, commonly known ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Northeast face of missile site control building, commonly known as the missile site radar building, showing open blast door #BD2. This emergency escape, at stair no. 12, is NEMP/RFI-shielded and 16" thick. The large circle in the center is the radar face, also known as the antennae array aperture. The small circle to the right of the radar face is the "Q" channel. The antennae atop the turret provided lightning protection for the building - Stanley R. Mickelsen Safeguard Complex, Missile Site Control Building, Northeast of Tactical Road; southeast of Tactical Road South, Nekoma, Cavalier County, ND

  18. Identification of adjacent binding sites for the YY1 and E4BP4 transcription factors in the ovine PrP (Prion) gene promoter.

    PubMed

    Burgess, Stewart T G; Shen, Cuicui; Ferguson, Laura A; O'Neill, Gerard T; Docherty, Kevin; Hunter, Nora; Goldmann, Wilfred

    2009-03-13

    The PrP gene encodes the cellular isoform of the prion protein (PrP(c)) which has been shown to be crucial to the development of transmissible spongiform encephalopathies (TSEs). PrP knock-out mice, which do not express endogenous PrP(c), exhibit resistance to TSE disease. The regulation of PrP gene expression represents, therefore, a crucial factor in the development of TSEs. Two sequence motifs in the PrP promoter (positions -287 to -263 from transcriptional start) were previously reported as being highly conserved, and it was suggested that they represent binding sites for as yet unidentified transcription factors. To test this hypothesis, binding of nuclear proteins was analyzed by electrophoretic mobility shift assays using ovine or murine cells and tissues with radiolabeled DNA probes containing the conserved motif sequences. Specific binding was observed to both motifs, and polymorphic variants of these motifs exhibited differential binding. Two proteins bound to these motifs were identified as the Yin Yang 1 (YY1) (motif 1) and E4BP4 (motif 2) transcription factors. Functional promoter analysis of four different promoter variants revealed that motif 1 (YY1) was associated with inhibitory activity in the context of the PrP promoter, whereas motif 2 (E4BP4) was linked to a slight enhancing activity. This represents the first demonstration of binding of nuclear factors to two highly conserved DNA sequence motifs within mammalian PrP promoters. The action of these factors on the PrP promoter is haplotype-specific, leading us to propose that the prion protein expression pattern and, with it, the distribution of TSE infectivity may be associated with PrP promoter genotype. PMID:19129193

  19. Thyroid abnormality trend over time in northeastern regions of Kazakstan, adjacent to the Semipalatinsk nuclear test site: a case review of pathological findings for 7271 patients.

    PubMed

    Zhumadilov, Z; Gusev, B I; Takada, J; Hoshi, M; Kimura, A; Hayakawa, N; Takeichi, N

    2000-03-01

    From 1949 through 1989 nuclear weapons testing carried out by the former Soviet Union at the Semipalatinsk Nuclear Test Site (SNTS) resulted in local fallout affecting the residents of Semipalatinsk, Ust-Kamenogorsk and Pavlodar regions of Kazakstan. To investigate the possible relationship between radiation exposure and thyroid gland abnormalities, we conducted a case review of pathological findings of 7271 urban and rural patients who underwent surgery from 1966-96. Of the 7271 patients, 761 (10.5%) were men, and 6510 (89.5%) were women. The age of the patients varied from 15 to 90 years. Overall, a diagnosis of adenomatous goiter (most frequently multinodular) was found in 1683 patients (63.4%) of Semipalatinsk region, in 2032 patients (68.6%) of Ust-Kamenogorsk region and in 1142 patients (69.0%) of Pavlodar region. In the period 1982-96, as compared before, there was a noticeable increase in the number of cases of Hashimoto's thyroiditis and thyroid cancer. Among histological forms of thyroid cancer, papillary (48.1%) and follicular (33.1%) predominated in the Semipalatinsk region. In later periods (1987-96), an increased frequency of abnormal cases occurred among patients less than 40 years of age, with the highest proportion among patients below 20 in Semipalatinsk and Ust-Kamenogorsk regions of Kazakstan. Given the positive findings of a significant cancer-period interaction, and a significant trend for the proportion of cancer to increase over time, we recommend more detailed and etiologic studies of thyroid disease among populations exposed to radiation fallout from the SNTS in comparison to non-exposed population. PMID:10838808

  20. [Unusual motifs of the nucleotide sequence adjacent to the putative transcription initiation site in the rDNA intergenic spacer of diploid wheat Triticum urartu Thum. ex Gandil, T. boeoticum Boiss, and T. monococcum L].

    PubMed

    Akhunov, E D; Chemeris, A V; Vakhitov, V A

    1997-11-01

    In the intergenic spacer (IGS) of rDNA of diploid wheats Triticum urartu, T. boeoticum, and T. monococcum, the uncommon motives adjacent to the site of transcription initiation (TIS) are revealed. They are located in the region from -6 to +1 relative to the putative TIS and are not encountered in cereals studied earlier. In T. urartu and T. boeoticum, the motif TACTATG has been revealed, in T. monococcum--TATTATG, while diploid Aegilops speltoides has the motif TATAGTA, typical of the remaining cereal species. The TIS-surrounding rDNA IGS region of diploid wheats was compared to the correspondent known rDNA IGS regions of different plant and animal species. PMID:9480224

  1. Near-Surface Geologic Units Exposed Along Ares Vallis and in Adjacent Areas: A Potential Source of Sediment at the Mars Pathfinder Landing Site

    NASA Technical Reports Server (NTRS)

    Treiman, Allan H.

    1997-01-01

    A sequence of layers, bright and dark, is exposed on the walls of canyons, impact craters and mesas throughout the Ares Vallis region, Chryse Planitia, and Xanthe Terra, Mars. Four layers can be seen: two pairs of alternating dark and bright albedo. The upper dark layer forms the top surface of many walls and mesas. The upper dark-bright pair was stripped as a unit from many streamlined mesas and from the walls of Ares Valles, leaving a bench at the top of the lower dark layer, approximately 250 m below the highland surface on streamlined islands and on the walls of Ares Vallis itself. Along Ares Vallis, the scarp between the highlands surface and this bench is commonly angular in plan view (not smoothly curving), suggesting that erosion of the upper dark-bright pair of layers controlled by planes of weakness, like fractures or joints. These near-surface layers in the Ares Vallis area have similar thicknesses, colors, and resistances to erosion to layers exposed near the tops of walls in Valles Marineris (Treiman et al.) and may represent the same pedogenic hardpan units. From this correlation, and from analogies with hardpans on Earth, the light-color layers may be cemented by calcite or gypsum. The dark layers are likely cemented by an iron-bearing mineral. Mars Pathfinder instruments should permit recognition and useful analyses of hardpan fragments, provided that clean uncoated surfaces are accessible. Even in hardpan-cemented materials, it should be possible to determine the broad types of lithologies in the Martian highlands. However, detailed geochemical modeling of highland rocks and soils may be compromised by the presence of hardpan cement minerals.

  2. Hanford Site surface soil radioactive contamination control plan, March 1993

    SciTech Connect

    Mix, P.D.; Winship, R.A.

    1993-04-01

    The Decommissioning and Resource Conservation and Recovery Act Closure Program is responsible to the US Department of Energy Richland Field Office, for the safe and cost-effective surveillance, maintenance, and decommissioning of surplus facilities and Resource Conservation and Recovery Act of 1976 closures at the Hanford Site. This program also manages the Radiation Area Remedial Action that includes the surveillance, maintenance, decontamination, and/or interim stabilization of inactive burial grounds, cribs, ponds, trenches, and unplanned release sites. This plan addresses only the Radiation Area Remedial Action activity requirements for managing and controlling the contaminated surface soil areas associated with these inactive sites until they are remediated as part of the Hanford Site environmental restoration process. All officially numbered Radiation Area Remedial Action and non-Radiation Area Remedial Action contaminated surface soil areas are listed in this document so that a complete list of the sites requiring remediation is contained in one document.

  3. Difference of Ecosystem and Hydrological control on Long-term water quality between adjacent subcatchments in a forested catchment in central Japan

    NASA Astrophysics Data System (ADS)

    Katsuyama, M.; Iwasaki, K.; Nagano, R.; Takaki, K.; Tanaka, Y.

    2014-12-01

    We have been monitoring the water quality in the Kiryu Experimental Watershed (KEW; 5.99 ha), Japan, and four subcatchments for more than 20 years. The climate of KEW is warm temperate. The artificially planted Japanese Cypress around 1960 covers whole of the KEW. The geology of KEW is weathered granite. The concentrations of SiO2 and Na, which are mainly supplied from weathering processes, in streamwater were different between the catchments, and the difference was controlled by hydrological conditions in each catchment; in the catchment where shallow groundwater contribution to the streamflow is large, the concentrations were lower. Conversely, in the catchment where deep, bedrock groundwater contribution is large, these concentrations were higher. The K+ concentration which cycles between soil and plants showed clear seasonal variations and the differences between the catchments were small. Considering the long-term trend of streamwater chemistry, the electric conductivity (EC), Cl-, and SO42- concentrations have been gradually decreasing during recent 10 years in all catchments. On the other hand, the NO3- concentration has been commonly increasing in recent 5 years in all catchment. The difference of concentrations between the catchments were depending on the difference of the redox condition caused by soil sediment. One of the subcatchments experienced the disturbance in early 90's and subsequent increase of NO3- concentration, and the residence tine distribution shows the effects of the disturbance is still remaining. Recently, the disturbance is expanding in another subcatchment, and one of the reason of the disturbance is soil erosion. Moreover, the streamwater NO3- concentrations are also gradually increasing even in undisturbed catchments. These facts imply that this 50-year-old unmanaged artificial forest may start degrading, and the biogeochemical cycle may start changing. The long-term dynamics of streamwater chemistry is a good diagnosis tool of the

  4. Spatial and temporal variations and controlling factors of sediment accumulation in the Yangtze River estuary and its adjacent sea area in the Holocene, especially in the Early Holocene

    NASA Astrophysics Data System (ADS)

    Feng, Zhibing; Liu, Baohua; Zhao, Yuexia; Li, Xishuang; Jiang, Li; Si, Shaokun

    2016-08-01

    The sub-bottom and collected borehole data provide insight into the transport and accumulation processes of the Yangtze-derived sediment in the study area since ~11 kyr BP. Five seismic units were identified according to six major acoustic surfaces. The sedimentary strata consist of fluvial, estuarine and deltaic systems from the bottom up, characterized by two different trends in sediment accumulation rates, i.e., low-high-low, and high-low-high. On the inner shelf of the East China Sea, the terrain with trough and ridge was formed by the Early Holocene transgression strata (formed in ~10 to 12 kyr BP) scoured by the later rectilinear tidal current due to postglacial sea-level transgression, and the sharply protruding seismic units are interpreted to be bedrocks outcropping on the seafloor. An analysis of the sedimentary characteristics in the boreholes and such factors as difference in accumulation rates, and tectonic subsidence led us to conclude that the paleo-coastline was located not far away from and to the east of Core ZK09 at ~9 kyr BP, and the southern bank of the Yangtze River estuary was located to the south of Core ZK09. At ~9 kyr, large volume of sediments was deposited in the northern isles of the Zhoushan archipelago and their adjacent bedrocks, forming a barrier effect on later sediment transport. During 7.5-8 kyr BP, the Yangtze-derived sediments were transported eastwards along the southern bank of the Yangtze River and the barrier due to the influence of the paleo-coastal current from the north, the direction of the Yangtze-derived sediment transport was split on the northeast of the Zhoushan archipelago, and the sediments covered the terrain with trough and ridge. During the high sea level period (7 kyr BP-present), the eastward migration of paleo-coastline had resulted in the increase in accumulation rate. We also conclude that the sharp increase in accumulation rate near the Yangtze River estuary after ~2 kyr BP was not primarily caused by

  5. Exposure Controls for Nanomaterials at Three Manufacturing Sites

    PubMed Central

    Heitbrink, William A.; Dunn, Kevin H.

    2015-01-01

    Because nanomaterials are thought to be more biologically active than their larger parent compounds, careful control of exposures to nanomaterials is recommended. Field studies were conducted at three sites to develop information about the effectiveness of control measures including process changes, a downflow room, a ventilated enclosure, and an enclosed reactor. Aerosol mass and number concentrations were measured during specific operations with a photometer and an electrical mobility particle sizer to provide concentration measurements across a broad range of sizes (from 5.6 nm to 30 μm). At site A, the dust exposure and during product harvesting was eliminated by implementing a wait time of 30 minutes following process completion. And, the dust exposure attributed to process tank cleaning was reduced from 0.7 to 0.2 mg/m3 by operating the available process ventilation during this task. At site B, a ventilated enclosure was used to control dust generated by the manual weigh-out and manipulation of powdered nanomaterials inside of a downflow room. Dust exposures were at room background (under 0.04 mg/m3 and 500 particles/cm3) during these tasks however, manipulations conducted outside of the enclosure were correlated with a transient increase in concentration measured at the source. At site C, a digitally controlled reactor was used to produce aligned carbon nanotubes. This reactor was a closed system and the ventilation functioned as a redundant control measure. Process emissions were well controlled by this system with the exception of increased concentrations measured during the unloading the product. However, this emission source could be easily controlled through increasing cabinet ventilation. The identification and adoption of effective control technologies is an important first step in reducing the risk associated with worker exposure to engineered nanoparticles. Properly designing and evaluating the effectiveness of these controls is a key component in

  6. Exposure controls for nanomaterials at three manufacturing sites.

    PubMed

    Heitbrink, William A; Lo, Li-Ming; Dunn, Kevin H

    2015-01-01

    Because nanomaterials are thought to be more biologically active than their larger parent compounds, careful control of exposures to nanomaterials is recommended. Field studies were conducted at three sites to develop information about the effectiveness of control measures including process changes, a downflow room, a ventilated enclosure, and an enclosed reactor. Aerosol mass and number concentrations were measured during specific operations with a photometer and an electrical mobility particle sizer to provide concentration measurements across a broad range of sizes (from 5.6 nm to 30 μm). At site A, the dust exposure and during product harvesting was eliminated by implementing a wait time of 30 -min following process completion. And, the dust exposure attributed to process tank cleaning was reduced from 0.7 to 0.2 mg/m3 by operating the available process ventilation during this task. At site B, a ventilated enclosure was used to control dust generated by the manual weigh-out and manipulation of powdered nanomaterials inside of a downflow room. Dust exposures were at room background (under 0.04 mg/m3 and 500 particles/cm3) during these tasks however, manipulations conducted outside of the enclosure were correlated with a transient increase in concentration measured at the source. At site C, a digitally controlled reactor was used to produce aligned carbon nanotubes. This reactor was a closed system and the ventilation functioned as a redundant control measure. Process emissions were well controlled by this system with the exception of increased concentrations measured during the unloading of the product. However, this emission source could be easily controlled through increasing cabinet ventilation. The identification and adoption of effective control technologies is an important first step in reducing the risk associated with worker exposure to engineered nanoparticles. Properly designing and evaluating the effectiveness of these controls is a key component in

  7. Controls on suspended sediment, particulate and dissolved organic carbon export from two adjacent catchments with contrasting land-uses, Exmoor UK.

    NASA Astrophysics Data System (ADS)

    Glendell, M.; Brazier, R. E.

    2012-04-01

    The fluvial export of total organic carbon (particulate and dissolved) plays an important role in the transportation of organic carbon from terrestrial to aquatic ecosystems, with implications for the understanding of the global carbon cycle and calculations of regional carbon budgets. The terrestrial biosphere contains large amounts of stored carbon in the soil and vegetation, thus a small change in the terrestrial carbon pool may have significant implications for atmospheric CO2 concentrations. Since the onset of agriculture, human activities have accelerated soil erosion rates 10- to 100- fold above all estimated natural background levels, especially in the uplands and at lower latitudes, whilst increasing DOC concentrations over the past decades have been reported in rivers across Western Europe and North America, raising concerns about potential destabilisation of the terrestrial soil carbon pool. The increased input of fine sediment and organic carbon into aquatic environments is also an important factor in stream water quality, being responsible for direct ecological effects as well as transport of a range of contaminants. Many factors, such as topography, hydrological regime and vegetation are known to influence the fluvial export of carbon from catchments. However, most work to date has focused on DOC losses from either forested or peaty catchments, with only limited studies examining the controls and rates of TOC (dissolved and particulate) fluxes from agricultural catchments, particularly during flood events. This research aims to: • Quantify the fluxes of total suspended sediment, total dissolved and total particulate carbon in two adjacent catchments with contrasting land-uses and • Examine the controlling factors of total fluvial carbon fluxes in a semi-natural and agricultural catchment in order to assess the impact of agricultural land-use on fluvial carbon export. The two contrasting study catchments (the Aller and Horner), in south

  8. 3. View from missile site control building (southeast to northwest) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. View from missile site control building (southeast to northwest) of missile launch area showing universal missile building on left and warhead handling building in background. - Stanley R. Mickelsen Safeguard Complex, Missile Launch Area, Within Exclusion Area, Nekoma, Cavalier County, ND

  9. Nevada Test Site Radiological Control Manual. Revision 1

    SciTech Connect

    None, None

    2010-02-09

    This document supersedes DOE/NV/25946--801, “Nevada Test Site Radiological Control Manual,” Revision 0 issued in October 2009. Brief Description of Revision: A minor revision to correct oversights made during revision to incorporate the 10 CFR 835 Update; and for use as a reference document for Tenant Organization Radiological Protection Programs.

  10. Controllable Magnetization Processes Induced by Nucleation Sites in Permalloy Rings

    NASA Astrophysics Data System (ADS)

    Ying-Jiun Chen,; Chia-Jung Hsu,; Chun-Neng Liao,; Hao-Ting Huang,; Chiun-Peng Lee,; Yi-Hsun Chiu,; Tzu-Yun Tung,; Mei-Feng Lai,

    2010-02-01

    Different arrangements of notches as nucleation sites are demonstrated experimentally and numerically to effectively control the magnetization processes of permalloy rings. In the ring with notches at the same side with respect to field direction, two same-helicity vortex domain walls in the onion state lead to two-step switching going through flux-closure state; in the ring with diagonal notches two opposite-helicity vortex domain walls lead to one-step switching skipping flux-closure state. The switching processes are repeatable in contrast to rings without notches where helicites of two vortex domain walls are random so the switching processes can not be controlled.

  11. Identification of a large bent DNA domain and binding sites for serum response factor adjacent to the NFI repeat cluster and enhancer region in the major IE94 promoter from simian cytomegalovirus.

    PubMed Central

    Chang, Y N; Jeang, K T; Chiou, C J; Chan, Y J; Pizzorno, M; Hayward, G S

    1993-01-01

    The major immediate-early (MIE) transactivator proteins of cytomegaloviruses (CMV) play a pivotal role in the initiation of virus-host cell interactions. Therefore, cis- and trans-acting factors influencing the expression of these proteins through their upstream promoter-enhancer regions are important determinants of the outcome of virus infection. S1 nuclease analysis and in vitro transcription assays with the MIE (or IE94) transcription unit of simian CMV (SCMV) (Colburn) revealed a single prominent mRNA start site associated with a canonical TATATAA motif. This initiator region lies adjacent to a 2,400-bp 5'-upstream noncoding sequence that encompasses a newly identified 1,000-bp (A+T)-rich segment containing intrinsically bent DNA (domain C), together with the previously described proximal cyclic AMP response element locus (domain A) and a tandemly repeated nuclear factor I binding site cluster (domain B). Deleted MIE reporter gene constructions containing domain A sequences only yield up to 4-fold stronger basal expression in Vero cells than the intact simian virus 40 promoter-enhancer region, and sequences from position -405 to -69 (ENH-A1) added to a minimal heterologous promoter produced a 50-fold increase of basal expression in an enhancer assay. In contrast, neither the nuclear factor I cluster nor the bent DNA region possessed basal enhancer properties and neither significantly modulated the basal activity of the ENH-A1 segment. A second segment of domain A from position -580 to -450 was also found to possess basal enhancer activity in various cell types. This ENH-A2 region contains three copies of a repeated element that includes the 10-bp palindromic sequence CCATATATGG, which resembles the core motif of serum response elements and proved to bind specifically to the cellular nuclear protein serum response transcription factor. Reporter gene constructions containing four tandem copies of these elements displayed up to 13-fold increased basal enhancer

  12. SOLIDS TRANSPORT BETWEEN ADJACENT CAFB FLUIDIZED BEDS

    EPA Science Inventory

    The report gives results of an experimental investigation of a pulsed, dense-phase pneumatic transport system for controlled circulation between adjacent fluidized beds. A model was developed to predict performance. The program provides technical support for EPA's program to demo...

  13. Design control for the Savannah River Site Consolidated Incineration Facility

    SciTech Connect

    Walker, R.E.; Rider, R.L.

    1991-12-31

    The initiation, development, and control of the design for the Consolidated Incineration Facility at the US Department of Energy Savannah River Site has been, from the inception, a precisely and formally controlled process. A plan was developed and implemented to ensure output properly aligned with approved design criteria and conformed to applicable regulations throughout the design process. The key element of design control was the technical baseline which established the benchmark against which all changes to the design was evaluated. During the conceptual design phase of the project, design criteria were written to reflect the project objectives and functional requirements. Governmental regulations were reviewed to determine permitting and licensing actions required. Hazards assessments were performed to establish design classifications. The resulting design criteria, permitting requirements, and facility classifications were incorporated into the design plan which provided the basis for subsequent design activities. As the project proceeded through the various design phases, design control was maintained according to the design plan. Review of all design products was performed by the project team routinely. Formal independent design reviews were accomplished prior to releasing the design for construction. Alignment between criteria and design output was verified periodically throughout the design process. A formal design change control board was invoked to effect design changes impacting technical baselines. All changes to design initiated following issue for construction also were subject to procedural control.

  14. Design control for the Savannah River Site Consolidated Incineration Facility

    SciTech Connect

    Walker, R.E.; Rider, R.L. )

    1991-01-01

    The initiation, development, and control of the design for the Consolidated Incineration Facility at the US Department of Energy Savannah River Site has been, from the inception, a precisely and formally controlled process. A plan was developed and implemented to ensure output properly aligned with approved design criteria and conformed to applicable regulations throughout the design process. The key element of design control was the technical baseline which established the benchmark against which all changes to the design was evaluated. During the conceptual design phase of the project, design criteria were written to reflect the project objectives and functional requirements. Governmental regulations were reviewed to determine permitting and licensing actions required. Hazards assessments were performed to establish design classifications. The resulting design criteria, permitting requirements, and facility classifications were incorporated into the design plan which provided the basis for subsequent design activities. As the project proceeded through the various design phases, design control was maintained according to the design plan. Review of all design products was performed by the project team routinely. Formal independent design reviews were accomplished prior to releasing the design for construction. Alignment between criteria and design output was verified periodically throughout the design process. A formal design change control board was invoked to effect design changes impacting technical baselines. All changes to design initiated following issue for construction also were subject to procedural control.

  15. 33. HISTORIC PLAQUE MARKING WHERE JOHNSTON DIED, ADJACENT TO PATHWAY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    33. HISTORIC PLAQUE MARKING WHERE JOHNSTON DIED, ADJACENT TO PATHWAY WITH CONCRETE CULVERT LEADING NORTH OUT OF RAVINE TOWARD JOHNSTON MEMORIAL SITE. VIEW NW. - Shiloh National Military Park Tour Roads, Shiloh, Hardin County, TN

  16. Preconception maternal nutrition: a multi-site randomized controlled trial

    PubMed Central

    2014-01-01

    Background Research directed to optimizing maternal nutrition commencing prior to conception remains very limited, despite suggestive evidence of its importance in addition to ensuring an optimal nutrition environment in the periconceptional period and throughout the first trimester of pregnancy. Methods/Study design This is an individually randomized controlled trial of the impact on birth length (primary outcome) of the time at which a maternal nutrition intervention is commenced: Arm 1: ≥ 3 mo preconception vs. Arm 2: 12-14 wk gestation vs. Arm 3: none. 192 (derived from 480) randomized mothers and living offspring in each arm in each of four research sites (Guatemala, India, Pakistan, Democratic Republic of the Congo). The intervention is a daily 20 g lipid-based (118 kcal) multi-micronutient (MMN) supplement. Women randomized to receive this intervention with body mass index (BMI) <20 or whose gestational weight gain is low will receive an additional 300 kcal/d as a balanced energy-protein supplement. Researchers will visit homes biweekly to deliver intervention and monitor compliance, pregnancy status and morbidity; ensure prenatal and delivery care; and promote breast feeding. The primary outcome is birth length. Secondary outcomes include: fetal length at 12 and 34 wk; incidence of low birth weight (LBW); neonatal/infant anthropometry 0-6 mo of age; infectious disease morbidity; maternal, fetal, newborn, and infant epigenetics; maternal and infant nutritional status; maternal and infant microbiome; gut inflammatory biomarkers and bioactive and nutritive compounds in breast milk. The primary analysis will compare birth Length-for-Age Z-score (LAZ) among trial arms (independently for each site, estimated effect size: 0.35). Additional statistical analyses will examine the secondary outcomes and a pooled analysis of data from all sites. Discussion Positive results of this trial will support a paradigm shift in attention to nutrition of all females of

  17. Control of Eolian soil erosion from waste site surface barriers

    SciTech Connect

    Ligotke, M.W.

    1994-11-01

    Physical models were tested in a wind tunnel to determine optimum surface-ravel admixtures for protecting silt-loam soil from erosion by, wind and saltating, sand stresses. The tests were performed to support the development of a natural-material surface barrier for and waste sites. Plans call for a 2-m deep silt-loam soil reservoir to retain infiltrating water from rainfall and snowmelt. The objective of the study was to develop a gravel admixture that would produce an erosion-resistant surface layer during, periods of extended dry climatic stress. Thus, tests were performed using simulated surfaces representing dry, unvegetated conditions present just after construction, after a wildfire, or during an extended drought. Surfaces were prepared using silt-loam soil mixed with various grades of sand and Travel. Wind-induced surface shear stresses were controlled over the test surfaces, as were saltating, sand mass flow rates and intensities. Tests were performed at wind speeds that approximated and exceeded local 100-year peak gust intensities. Surface armors produced by pea gravel admixtures were shown to provide the best protection from wind and saltating sand stresses. Compared with unprotected silt-loam surfaces, armored surfaces reduced erosion rates by more than 96%. Based in part on wind tunnel results, a pea gravel admixture of 15% will be added to the top 1 in of soil in a prototype barrier under construction in 1994. Field tests are planned at the prototype site to provide data for comparison with wind tunnel results.

  18. Optical properties of individual site-controlled Ge quantum dots

    SciTech Connect

    Grydlik, Martyna E-mail: martyna.grydlik@jku.at; Brehm, Moritz E-mail: martyna.grydlik@jku.at; Tayagaki, Takeshi; Langer, Gregor; Schäffler, Friedrich; Schmidt, Oliver G.

    2015-06-22

    We report photoluminescence (PL) experiments on individual SiGe quantum dots (QDs) that were epitaxially grown in a site-controlled fashion on pre-patterned Si(001) substrates. We demonstrate that the PL line-widths of single QDs decrease with excitation power to about 16 meV, a value that is much narrower than any of the previously reported PL signals in the SiGe/Si heterosystem. At low temperatures, the PL-intensity becomes limited by a 25 meV high potential-barrier between the QDs and the surrounding Ge wetting layer (WL). This barrier impedes QD filling from the WL which collects and traps most of the optically excited holes in this type-II heterosystem.

  19. Photocurrent spectroscopy of site-controlled pyramidal quantum dots

    NASA Astrophysics Data System (ADS)

    Mohan, A.; Nevou, L.; Gallo, P.; Dwir, B.; Rudra, A.; Kapon, E.; Faist, J.

    2012-07-01

    Intraband photocurrent spectroscopy of site-controlled pyramidal quantum dots by inserting them into the intrinsic region of n-i-n like quantum dot infrared photodetector structure is reported. The photovoltaic response is observed in the mid-infrared region. A peak responsivity of 0.4 mA/W at 120 meV (λ = 10 μm) is observed at 10 K at -2 V bias. The ability to engineer states in the conduction band of the QDs has been exploited to tune their photocurrent response from 10 μm to 18 μm with a narrow spectral width of Δλ/λ = 0.17.

  20. Use of controlled release herbicides in waste burial sites

    SciTech Connect

    Burton, F.G.; Cataldo, D.A.; Cline, J.F.; Skiens, W.E.

    1981-07-01

    Controlled-release formulations of herbicides have been applied to the soil in the manner traditional for herbicides: on the surface or mixed into the top few inches of soil. The controlled-release formulation allows another option that we propose to use: to place herbicides, contained in controlled-release formulations, in a layer at least a foot below the surface of the soil, in order to prevent root penetration below that level. Ideally, the herbicide will prevent root tip cell division but will not translocate within the plant, thus assuring that the plant will survive, preserving the ground cover. Trifluralin is one of the herbicides which does not translocate and was chosen for use in this study. A number of applications for this technology are possible; particularly in waste management. In the present studies, we used two different forms of polymeric carrier/delivery (PCD) systems to investigate the controlled release of herbicides. In the initial study, a sheet was made of homogeneous mixtures of an individual polymer and trifluralin. We made several of these sheets, using a different polymer each time (with trifluralin) to compare release rates from the various polymers. We also fabricated cylindrical pellets in two sizes from mixtures of Profax/sup a/ PS-1600 polypropylene and trifluralin, formulated to determine the interaction of PCD systems with soil. Also developed is a trifluralin-releasing device with a theoretical effective lifetime approaching 100 years. The system was designed specifically to protect the asphalt layer or clay/aggregate barriers on uranium mill tailings piles. PCD devices composed of pellets could also be implanted over burial sites for radioactive and/or toxic materials, preventing translocation of those materials to plant shoots, and thence into the biosphere.

  1. 2013 Annual Site Inspection and Monitoring Report for Uranium Mill Tailings Radiation Control Act Title I Disposal Sites

    SciTech Connect

    None, None

    2014-03-01

    This report, in fulfillment of a license requirement, presents the results of long-term surveillance and maintenance activities conducted by the U.S. Department of Energy (DOE) Office of Legacy Management (LM) in 2013 at 19 uranium mill tailings disposal sites established under Title I of the Uranium Mill Tailings Radiation Control Act (UMTRCA) of 1978.1 These activities verified that the UMTRCA Title I disposal sites remain in compliance with license requirements. DOE operates 18 UMTRCA Title I sites under a general license granted by the U.S. Nuclear Regulatory Commission (NRC) in accordance with Title 10 Code of Federal Regulations Part 40.27 (10 CFR 40.27). As required under the general license, a long-term surveillance plan (LTSP) for each site was prepared by DOE and accepted by NRC. The Grand Junction, Colorado, Disposal Site, one of the 19 Title I sites, will not be included under the general license until the open, operating portion of the cell is closed. The open portion will be closed either when it is filled or in 2023. This site is inspected in accordance with an interim LTSP. Long-term surveillance and maintenance services for these disposal sites include inspecting and maintaining the sites; monitoring environmental media and institutional controls; conducting any necessary corrective actions; and performing administrative, records, stakeholder relations, and other regulatory stewardship functions. Annual site inspections and monitoring are conducted in accordance with site-specific LTSPs and procedures established by DOE to comply with license requirements. Each site inspection is performed to verify the integrity of visible features at the site; to identify changes or new conditions that may affect the long-term performance of the site; and to determine the need, if any, for maintenance, follow-up or contingency inspections, or corrective action in accordance with the LTSP. LTSPs and site compliance reports are available on the Internet at http://www.lm.doe.gov/.

  2. A central role for a single c-Myb binding site in a thymic locus control region.

    PubMed Central

    Ess, K C; Whitaker, T L; Cost, G J; Witte, D P; Hutton, J J; Aronow, B J

    1995-01-01

    Locus control regions (LCRs) are powerful assemblies of cis elements that organize the actions of cell-type-specific trans-acting factors. A 2.3-kb LCR in the human adenosine deaminase (ADA) gene first intron, which controls expression in thymocytes, is composed of a 200-bp enhancer domain and extended flanking sequences that facilitate activation from within chromatin. Prior analyses have demonstrated that the enhancer contains a 28-bp core region and local adjacent augmentative cis elements. We now show that the core contains a single critical c-Myb binding site. In both transiently cotransfected human cells and stable chromatin-integrated yeast cells, c-Myb strongly transactivated reporter constructs that contained polymerized core sequences. c-Myb protein was strongly evident in T lymphoblasts in which the enhancer was active and was localized within discrete nuclear structures. Fetal murine thymus exhibited a striking concordance of endogenous c-myb expression with that of mouse ADA and human ADA LCR-directed transgene expression. Point mutation of the c-Myb site within the intact 2.3-kb LCR severely attenuated enhancer activity in transfections and LCR activity in transgenic thymocytes. Within the context of a complex enhancer and LCR, c-Myb can act as an organizer of thymocyte-specific gene expression via a single binding site. PMID:7565722

  3. 2013 Annual Site Inspection and Monitoring Report for Uranium Mill Tailings Radiation Control Act Title II Disposal Sites

    SciTech Connect

    2013-11-01

    This report, in fulfillment of a license requirement, presents the results of long-term surveillance and maintenance activities conducted by the U.S. Department of Energy (DOE) Office of Legacy Management in 2013 at six uranium mill tailings disposal sites reclaimed under Title II of the Uranium Mill Tailings Radiation Control Act (UMTRCA) of 1978. These activities verified that the UMTRCA Title II disposal sites remain in compliance with license requirements. DOE manages six UMTRCA Title II disposal sites under a general license granted by the U.S. Nuclear Regulatory Commission (NRC) established at Title 10 Code of Federal Regulations Part 40.28. Reclamation and site transition activities continue at other sites, and DOE ultimately expects to manage approximately 27 Title II disposal sites. Long-term surveillance and maintenance activities and services for these disposal sites include inspecting and maintaining the sites; monitoring environmental media and institutional controls; conducting any necessary corrective action; and performing administrative, records, stakeholder services, and other regulatory functions. Annual site inspections and monitoring are conducted in accordance with site-specific long-term surveillance plans (LTSPs) and procedures established by DOE to comply with license requirements. Each site inspection is performed to verify the integrity of visible features at the site; to identify changes or new conditions that may affect the long-term performance of the site; and to determine the need, if any, for maintenance, follow-up inspections, or corrective action. LTSPs and site compliance reports are available online at http://www.lm.doe.gov

  4. Spatial and temporal variations and controlling factors of sediment accumulation in the Yangtze River estuary and its adjacent sea area in the Holocene, especially in the Early Holocene

    NASA Astrophysics Data System (ADS)

    Feng, Zhibing; Liu, Baohua; Zhao, Yuexia; Li, Xishuang; Jiang, Li; Si, Shaokun

    2016-08-01

    The sub-bottom and collected borehole data provide insight into the transport and accumulation processes of the Yangtze-derived sediment in the study area since ~11 kyr BP. Five seismic units were identified according to six major acoustic surfaces. The sedimentary strata consist of fluvial, estuarine and deltaic systems from the bottom up, characterized by two different trends in sediment accumulation rates, i.e., low-high-low, and high-low-high. On the inner shelf of the East China Sea, the terrain with trough and ridge was formed by the Early Holocene transgression strata (formed in ~10 to 12 kyr BP) scoured by the later rectilinear tidal current due to postglacial sea-level transgression, and the sharply protruding seismic units are interpreted to be bedrocks outcropping on the seafloor. An analysis of the sedimentary characteristics in the boreholes and such factors as difference in accumulation rates, and tectonic subsidence led us to conclude that the paleo-coastline was located not far away from and to the east of Core ZK09 at ~9 kyr BP, and the southern bank of the Yangtze River estuary was located to the south of Core ZK09. At ~9 kyr BP, the Yangtze-derived sediments were transported eastwards along the southern bank of the Yangtze River and the barrier due to the influence of the paleo-coastal current from the north, the direction of the Yangtze-derived sediment transport was split on the northeast of the Zhoushan archipelago, and the sediments covered the terrain with trough and ridge. During the high sea level period (7 kyr BP-present), the eastward migration of paleo-coastline had resulted in the increase in accumulation rate. We also conclude that the sharp increase in accumulation rate near the Yangtze River estuary after ~2 kyr BP was not primarily caused by human activities. The position shifts of the estuary caused by the paleo-coastline migration and sea level oscillations since the Holocene is the main cause controlling the Yangtze

  5. Characterization of Hydraulically Significant Discontinuities in Mudrocks at the Waste Control Specialists (WCS) Site, West Texas

    NASA Astrophysics Data System (ADS)

    Kuszmaul, J. S.; Holt, R. M.; Powers, D. W.; Beauheim, R.; Pickens, J. F.; grisak, G. E.; Hughes, E.; Cook, S.

    2011-12-01

    Triassic mudrocks of the Dockum Group (Cooper Canyon Formation) host four, below-grade landfills at the Waste Control Specialists (WSC) site in Andrews County, Texas, including: a hazardous waste landfill and three radioactive waste landfills. At many radioactive waste disposal facilities, the long-term performance of the facility may be influenced by the transport of radionuclides through interconnected fracture networks. WCS developed an integrated geologic mapping and hydraulic testing program to evaluate the hydraulic significance of discontinuities within Dockum rocks. At the WCS site, the Dockum consists of mudrocks with sparse siltstone/sandstone interbeds that developed in a semi-arid environment from an ephemeral meandering fluvial system. Sedimentary studies reveal that the mudrocks are ancient floodplain vertisols (soils with swelling clays) and siltstone/sandstone interbeds are fluvial channel deposits that were frequently subaerially exposed. Rock discontinuities, including fractures, were mapped during the excavation of the WCS radioactive waste landfills along vertical faces prepared by the construction contractor. Face locations were selected to insure nearly complete vertical coverage for each landfill. Individual discontinuities were mapped and their strike, dip, length, roughness, curvature, staining, and evidence of displacement were described. In the three radioactive waste disposal landfills, over 1750 discontinuities across 35 excavated faces were mapped and described, where each face was nominally 8 to 10 ft tall and 50 to 100 ft long. On average, the orientation of the discontinuities was horizontal, and no other significant trends were observed. Mapping within the landfill excavations shows that most discontinuities within Dockum rocks are horizontal, concave upward, slickensided surfaces that developed in the depositional environment, as repeated wetting and drying cycles led to shrinking and swelling of floodplain vertisols. Fractures

  6. Strategies for cancer control on an organ-site basis.

    PubMed

    Moore, Malcolm A; Sobue, Tomotaka

    2010-01-01

    A great deal of research information has been generated regarding cancer incidence rates and underlying risk factors. Since incidence:mortality ratios are generally less than 2:1 and often approach equivalence there clearly is a need for particular emphasis on preventive measures and early detection. Whether the latter should be through screening or education for improved awareness will depend on the socioeconomic conditions and the organ site. The location within the body, physiological factors and the cell type, whether essentially glandular or squamous, and the particular risk and protective factors operating in the particular social context will all impact on what measures can be recommended. Here the focus is on primary and secondary prevention of cancers in the various regions of Asia, taking into account similarities and differences in etiology for organs/tissues of the gastrointestinal tract, the respiratory tract, the urinary system, the reproductive system, the nervous system, the thyroid and non-Hogkins lymphomas and leukemias. Globocan 2002 data on incidence and mortality and all of the findings reviewed in the Regional Reviews were taken into account in compiling this overview. The chief recommendations are education in the developing world, to overcome the problem of late presentation at hospital (reflected by high mortality/incidence ratios), betel and tobacco control for the oral cavity and pharynx, reduce salt intake and targeting of Helicobacter pylori for the stomach, reduction in food intake, improvement in the diet and more exercise for the colorectum, kidney, prostate, breast, ovary and endometrium, reduction in smoking and exposure to other fumes for the lung, increase in water intake, particularly for the urinary bladder, and avoidance of parasites for the special cases of the urinary bladder and intrahepatic bile ducts. The cancer registry could be a major resource for development of further research capacity, with selection of suitable

  7. AIR STRIPPERS AND THEIR EMISSIONS CONTROL AT SUPERFUND SITES

    EPA Science Inventory

    Air stripping, a traditional means of making slightly contaminated ground-water potable, is being applied increasingly to more severe groundwater pollution at remedial action sites. Concentrations of volatile and semivolatile compounds at such sites may reach hundreds of parts pe...

  8. Stable fly control in cattle winter feeding sites with Novaluron

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The mixture of wasted feed with manure and urine residues at livestock winter feeding sites provides an excellent substrate for the development of immature stable flies, Stomoxys calcitrans (L.). Such sites are primary sources of early summer stable flies in the central United States and limited opt...

  9. Geospatial methods for monitoring alternative control technology sites

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Soils data can be used to establish nutrient distribution at a selected site; however, soil sampling is labor intensive, costly, and selection of sampling sites may be difficult. This study was conducted to determine if methods developed for the management of saline soils can be applied to a vegetat...

  10. Control of incidental asbestos exposure at hazardous waste sites.

    PubMed

    Koustas, R N

    1991-07-01

    This paper discusses asbestos regulations that are not part of Superfund and examines how these regulations can help to identify, evaluate and manage the risk associated with Asbestos Containing Material (ACM) at hazardous waste cleanup sites. Unless one knows where to look for ACM at hazardous waste sites, it may go undetected even after all the traditional sampling is done. Although EPA is currently developing a policy for evaluating risk from asbestos exposure at certain Superfund sites, information from existing regulations can be used to manage hazards associated with asbestos exposure at hazardous waste sites. This paper also identifies where to find governmental agency personnel and consultants who may be retained for site-specific help. PMID:1930954

  11. Hedgerow restoration promotes pollinator populations and exports native bees to adjacent fields.

    PubMed

    Morandin, Lora A; Kremen, Claire

    2013-06-01

    In intensive agricultural landscapes, restoration within farms could enhance biodiversity and ecosystem services such as pollination by native pollinators. Although governments and conservation groups are promoting small-scale restoration on working farms, there are few studies that assess whether these practices enhance pollinator communities in restored areas. Further, there is no information on whether floral enhancements will deplete pollinators in adjacent fields by concentrating ambient populations or whether they result in a net increase in abundance in adjacent farm fields. We investigated whether field edges restored with native perennial plants in California's Central Valley agricultural region increased floral abundance and potential bee nesting sites, and native bee and syrphid fly abundance and diversity, in comparison to relatively unmanaged edges. Native bees and syrphid flies collected from flowers were more abundant, species-rich, and diverse at hedgerow sites than in weedy, unmanaged edges. Abundance of bees collected passively in pan traps was negatively correlated with floral abundance, was significantly different from communities captured by net sampling from flowers, and did not distinguish between site types; we therefore focused on the results of net samples and visual observations. Uncommon species of native bees were sevenfold more abundant on hedgerow flowers than on flowers at weedy, unmanaged edges. Of the species on flowers at hedgerows, 40% were exclusive to hedgerow sites, but there were no species exclusively found on flowers at control sites. Hedgerows were especially important for supporting less-common species of native bees in our intensive agricultural landscape. Hedgerows did not concentrate ambient native bee, honey bee, or syphid fly populations, and they acted as net exporters of native bees into adjacent fields. Within-farm habitat restoration such as hedgerow creation may be essential for enhancing native pollinator

  12. A new approach to a site radiological control manual.

    PubMed

    Farrell, Linda M

    2002-02-01

    The Pantex Plant significantly revised its Radiological Control Manual to improve its usability, streamline plant procedures, and clarify conflicting guidance. This article overviews the process used to develop the new Radiological Control Manual. PMID:11797900

  13. 10 CFR 960.4-2-8-2 - Site ownership and control.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... FOR A NUCLEAR WASTE REPOSITORY Postclosure Guidelines § 960.4-2-8-2 Site ownership and control. (a... requirements of 10 CFR part 60, ownership, surface and subsurface rights, and control of access that...

  14. 10 CFR 960.4-2-8-2 - Site ownership and control.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... FOR A NUCLEAR WASTE REPOSITORY Postclosure Guidelines § 960.4-2-8-2 Site ownership and control. (a... requirements of 10 CFR part 60, ownership, surface and subsurface rights, and control of access that...

  15. Control site location and transcriptional regulation in Escherichia coli.

    PubMed Central

    Collado-Vides, J; Magasanik, B; Gralla, J D

    1991-01-01

    The regulatory regions for 119 Escherichia coli promoters have been analyzed, and the locations of the regulatory sites have been cataloged. The following observations emerge. (i) More than 95% of promoters are coregulated with at least one other promoter. (ii) Virtually all sigma 70 promoters contain at least one regulatory site in a proximal position, touching at least position -65 with respect to the start point of transcription. There are not yet clear examples of upstream regulation in the absence of a proximal site. (iii) Operators within regulons appear in very variable proximal positions. By contrast, the proximal activation sites of regulons are much more fixed. (iv) There is a forbidden zone for activation elements downstream from approximately position -20 with respect to the start of transcription. By contrast, operators can occur throughout the proximal region. When activation elements appear in the forbidden zone, they repress. These latter examples usually involve autoregulation. (v) Approximately 40% of repressible promoters contain operator duplications. These occur either in certain regulons where duplication appears to be a requirement for repressor action or in promoters subject to complex regulation. (vi) Remote operator duplications occur in approximately 10% of repressible promoters. They generally appear when a multiple promoter region is coregulated by cyclic AMP receptor protein. (vii) Sigma 54 promoters do not require proximal or precisely positioned activator elements and are not generally subject to negative regulation. Rationales are presented for all of the above observations. PMID:1943993

  16. Radiological Control Technician: Phase 1, Site academic training lesson plans

    SciTech Connect

    Not Available

    1992-10-01

    This volume provides lesson plans for training radiological control technicians. Covered here is basic radiological documentation, counting errors, dosimetry, environmental monitoring, and radiation instruments.

  17. 21 CFR 1304.50 - Disclosure requirements for Web sites of nonpharmacy practitioners that dispense controlled...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 9 2014-04-01 2014-04-01 false Disclosure requirements for Web sites of nonpharmacy practitioners that dispense controlled substances by means of the Internet. 1304.50 Section 1304... that dispense controlled substances by means of the Internet. For a Web site to identify itself...

  18. 21 CFR 1304.50 - Disclosure requirements for Web sites of nonpharmacy practitioners that dispense controlled...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 9 2012-04-01 2012-04-01 false Disclosure requirements for Web sites of nonpharmacy practitioners that dispense controlled substances by means of the Internet. 1304.50 Section 1304... that dispense controlled substances by means of the Internet. For a Web site to identify itself...

  19. 21 CFR 1304.50 - Disclosure requirements for Web sites of nonpharmacy practitioners that dispense controlled...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 9 2013-04-01 2013-04-01 false Disclosure requirements for Web sites of nonpharmacy practitioners that dispense controlled substances by means of the Internet. 1304.50 Section 1304... that dispense controlled substances by means of the Internet. For a Web site to identify itself...

  20. 21 CFR 1304.50 - Disclosure requirements for Web sites of nonpharmacy practitioners that dispense controlled...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 9 2010-04-01 2010-04-01 false Disclosure requirements for Web sites of nonpharmacy practitioners that dispense controlled substances by means of the Internet. 1304.50 Section 1304... that dispense controlled substances by means of the Internet. For a Web site to identify itself...

  1. 21 CFR 1304.50 - Disclosure requirements for Web sites of nonpharmacy practitioners that dispense controlled...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 9 2011-04-01 2011-04-01 false Disclosure requirements for Web sites of nonpharmacy practitioners that dispense controlled substances by means of the Internet. 1304.50 Section 1304... that dispense controlled substances by means of the Internet. For a Web site to identify itself...

  2. 10 CFR 960.4-2-8-2 - Site ownership and control.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Site ownership and control. 960.4-2-8-2 Section 960.4-2-8-2 Energy DEPARTMENT OF ENERGY GENERAL GUIDELINES FOR THE PRELIMINARY SCREENING OF POTENTIAL SITES... requirements of 10 CFR part 60, ownership, surface and subsurface rights, and control of access that...

  3. 10 CFR 960.4-2-8-2 - Site ownership and control.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Site ownership and control. 960.4-2-8-2 Section 960.4-2-8-2 Energy DEPARTMENT OF ENERGY GENERAL GUIDELINES FOR THE PRELIMINARY SCREENING OF POTENTIAL SITES... requirements of 10 CFR part 60, ownership, surface and subsurface rights, and control of access that...

  4. 10 CFR 960.4-2-8-2 - Site ownership and control.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Site ownership and control. 960.4-2-8-2 Section 960.4-2-8-2 Energy DEPARTMENT OF ENERGY GENERAL GUIDELINES FOR THE PRELIMINARY SCREENING OF POTENTIAL SITES... requirements of 10 CFR part 60, ownership, surface and subsurface rights, and control of access that...

  5. Control of Pteridium aquilinum: Meta-analysis of a Multi-site Study in the UK

    PubMed Central

    Stewart, Gavin; Cox, Emma; Le Duc, Mike; Pakeman, Robin; Pullin, Andrew; Marrs, Rob

    2008-01-01

    Background and Aims A great deal of money is spent controlling invasive weeds as part of international and national policies. It is essential that the funded treatments work across the region in which the policies operate. We argue that experiments across multiple sites are required to validate these programs as results from single sites may be misleading. Here, the control of Pteridium aquilinum (bracken) is used as a test example to address the following four questions. (1) Does the effectiveness of P. aquilinum-control treatments vary across sites? (2) Is the best treatment identified in previous research (cutting twice per year) consistent at all sites, and if not why not? (3) Is treatment performance related to P. aquilinum rhizome mass, litter cover or litter depth at the various sites? (4) Does successful P. aquilinum control influence species richness? Methods Pteridium aquilinum‐control treatments were monitored for 10 years using six replicated experiments and analysed using meta-analysis. Meta-regressions were used to explore heterogeneity between sites. Key Results The effectiveness of treatments varied between sites depending on the measure used to assess P. aquilinum performance. In general, cutting twice per year was the most successful treatment but on some sites other, less expensive treatments were as good. The effectiveness of treatments at different sites was not related to rhizome mass, but the effectiveness of most applied treatments were inversely related to post-control litter. Effective treatment was also associated with high species richness. Conclusions It is concluded that successful development of national weed control programs requires multi-site experimental approaches. Here, meta-analyses demonstrate that variation in effectiveness between sites could be explained in part by pre-specified variables. Reliance on data from a single site for policy formulation is therefore clearly dangerous. PMID:18337356

  6. Cleavage Site Localization Differentially Controls Interleukin-6 Receptor Proteolysis by ADAM10 and ADAM17

    PubMed Central

    Riethmueller, Steffen; Ehlers, Johanna C.; Lokau, Juliane; Düsterhöft, Stefan; Knittler, Katharina; Dombrowsky, Gregor; Grötzinger, Joachim; Rabe, Björn; Rose-John, Stefan; Garbers, Christoph

    2016-01-01

    Limited proteolysis of the Interleukin-6 Receptor (IL-6R) leads to the release of the IL-6R ectodomain. Binding of the cytokine IL-6 to the soluble IL-6R (sIL-6R) results in an agonistic IL-6/sIL-6R complex, which activates cells via gp130 irrespective of whether the cells express the IL-6R itself. This signaling pathway has been termed trans-signaling and is thought to mainly account for the pro-inflammatory properties of IL-6. A Disintegrin And Metalloprotease 10 (ADAM10) and ADAM17 are the major proteases that cleave the IL-6R. We have previously shown that deletion of a ten amino acid long stretch within the stalk region including the cleavage site prevents ADAM17-mediated cleavage, whereas the receptor retained its full biological activity. In the present study, we show that deletion of a triple serine (3S) motif (Ser-359 to Ser-361) adjacent to the cleavage site is sufficient to prevent IL-6R cleavage by ADAM17, but not ADAM10. We find that the impaired shedding is caused by the reduced distance between the cleavage site and the plasma membrane. Positioning of the cleavage site in greater distance towards the plasma membrane abrogates ADAM17-mediated shedding and reveals a novel cleavage site of ADAM10. Our findings underline functional differences in IL-6R proteolysis by ADAM10 and ADAM17. PMID:27151651

  7. 46 CFR 148.445 - Adjacent spaces.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 5 2013-10-01 2013-10-01 false Adjacent spaces. 148.445 Section 148.445 Shipping COAST... THAT REQUIRE SPECIAL HANDLING Additional Special Requirements § 148.445 Adjacent spaces. When... following requirements must be met: (a) Each space adjacent to a cargo hold must be ventilated by...

  8. 46 CFR 148.445 - Adjacent spaces.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 5 2014-10-01 2014-10-01 false Adjacent spaces. 148.445 Section 148.445 Shipping COAST... THAT REQUIRE SPECIAL HANDLING Additional Special Requirements § 148.445 Adjacent spaces. When... following requirements must be met: (a) Each space adjacent to a cargo hold must be ventilated by...

  9. 46 CFR 148.445 - Adjacent spaces.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 5 2012-10-01 2012-10-01 false Adjacent spaces. 148.445 Section 148.445 Shipping COAST... THAT REQUIRE SPECIAL HANDLING Additional Special Requirements § 148.445 Adjacent spaces. When... following requirements must be met: (a) Each space adjacent to a cargo hold must be ventilated by...

  10. 46 CFR 148.445 - Adjacent spaces.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 5 2011-10-01 2011-10-01 false Adjacent spaces. 148.445 Section 148.445 Shipping COAST... THAT REQUIRE SPECIAL HANDLING Additional Special Requirements § 148.445 Adjacent spaces. When... following requirements must be met: (a) Each space adjacent to a cargo hold must be ventilated by...

  11. Large-scale control site selection for population monitoring: an example assessing Sage-grouse trends

    USGS Publications Warehouse

    Fedy, Bradley C.; O'Donnell, Michael; Bowen, Zachary H.

    2015-01-01

    Human impacts on wildlife populations are widespread and prolific and understanding wildlife responses to human impacts is a fundamental component of wildlife management. The first step to understanding wildlife responses is the documentation of changes in wildlife population parameters, such as population size. Meaningful assessment of population changes in potentially impacted sites requires the establishment of monitoring at similar, nonimpacted, control sites. However, it is often difficult to identify appropriate control sites in wildlife populations. We demonstrated use of Geographic Information System (GIS) data across large spatial scales to select biologically relevant control sites for population monitoring. Greater sage-grouse (Centrocercus urophasianus; hearafter, sage-grouse) are negatively affected by energy development, and monitoring of sage-grouse population within energy development areas is necessary to detect population-level responses. Weused population data (1995–2012) from an energy development area in Wyoming, USA, the Atlantic Rim Project Area (ARPA), and GIS data to identify control sites that were not impacted by energy development for population monitoring. Control sites were surrounded by similar habitat and were within similar climate areas to the ARPA. We developed nonlinear trend models for both the ARPA and control sites and compared long-term trends from the 2 areas. We found little difference between the ARPA and control sites trends over time. This research demonstrated an approach for control site selection across large landscapes and can be used as a template for similar impact-monitoring studies. It is important to note that identification of changes in population parameters between control and treatment sites is only the first step in understanding the mechanisms that underlie those changes. Published 2015. This article is a U.S. Government work and is in the public domain in the USA.

  12. Geospatial methods for monitoring alternative control technology sites

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Monitoring alternative feedlot runoff control technology effectiveness, especially vegetative treatment systems (VTS), is of interest to both cattlemen and regulatory agencies. Producers have constructed VTS in several mid-western states under an agreement with the Iowa Cattlemen Association and the...

  13. Probable maximum flood control; Yucca Mountain Site Characterization Project

    SciTech Connect

    DeGabriele, C.E.; Wu, C.L.

    1991-11-01

    This study proposes preliminary design concepts to protect the waste-handling facilities and all shaft and ramp entries to the underground from the probable maximum flood (PMF) in the current design configuration for the proposed Nevada Nuclear Waste Storage Investigation (NNWSI) repository protection provisions were furnished by the United States Bureau of Reclamation (USSR) or developed from USSR data. Proposed flood protection provisions include site grading, drainage channels, and diversion dikes. Figures are provided to show these proposed flood protection provisions at each area investigated. These areas are the central surface facilities (including the waste-handling building and waste treatment building), tuff ramp portal, waste ramp portal, men-and-materials shaft, emplacement exhaust shaft, and exploratory shafts facility.

  14. Malaria Vectors in Lake Victoria and Adjacent Habitats in Western Kenya

    PubMed Central

    Minakawa, Noboru; Dida, Gabriel O.; Sonye, George O.; Futami, Kyoko; Njenga, Sammy M.

    2012-01-01

    The prevalence of malaria among the residents of the Lake Victoria basin remains high. The environment associated with the lake may maintain a high number of malaria vectors. Lake habitats including water hyacinths have been suspected to be the source of vectors. This study investigated whether malaria vectors breed in the lake habitats and adjacent backwater pools. Anopheline larvae were collected within the littoral zone of the lake and adjacent pools located along approximately 24.3 km of the lakeshore in western Kenya, and their breeding sites characterized. Three primary vector species, Anopheles arabiensis, Anopheles gambiae s.s. and Anopheles funestus s.s., and three potential vectors, were found in the lake habitats. Unexpectedly, An. arabiensis was the most dominant vector species in the lake sampling sites. Its habitats were uncovered or covered with short grass. A potential secondary malaria vector, Anopheles rivulorum, dominated the water hyacinths in the lake. Most breeding sites in the lake were limited to areas that were surrounded by tall emergent plants, including trees, and those not exposed to waves. Nearly half of adjacent habitats were lagoons that were separated from the lake by sand bars. Lagoons contained a variety of microhabitats. Anopheles arabiensis dominated open habitats, whereas An. funestus s.s. was found mainly in vegetated habitats in lagoons. The current study confirmed that several breeding sites are associated with Lake Victoria. Given that Lake Victoria is the second largest lake in the world, the lake related habitats must be extensive; therefore, making targeted vector control difficult. Further exploration is necessary to estimate the effects of lake associated habitats on malaria transmission so as to inform a rational decision-making process for vector control. PMID:22412913

  15. Remote controlled tool systems for nuclear sites have subsea applications

    SciTech Connect

    Bath, B.; Yemington, C.; Kuhta, B.

    1995-10-01

    Remotely operated underwater tool systems designed to operate in Nuclear Fuel Storage Basins can be applied to deep water, subsea oilfield applications. Spent nuclear fuel rods re stored underwater in large indoor swimming pool-like facilities where the water cover shields the workers from the radiation. This paper describes three specialized tooling systems that were designed and built by Sonsub for work at the Department of Energy`s Hanford site. The Door Seal Tool removed an existing seal system, cleaned a 20 ft. tall, carbon steel, underwater hatch and installed a new stainless steel gasket surface with underwater epoxy. The Concrete Sampling Tool was built to take core samples from the vertical, concrete walls of the basins. The tool has three hydraulic drills with proprietary hollow core drill bits to cut and retrieve the concrete samples. The Rack Saw remotely attached itself to a structure, cut a variety of steel shapes and pipes, and retained the cut pieces for retrieval. All of these systems are remotely operated with onboard video cameras and debris collection systems. The methods and equipment proven in this application are available to refurbish sealing surfaces and to drill or sample concrete in offshore oil field applications.

  16. A sequence-specific DNA-binding factor (VF1) from Anabaena sp. strain PCC 7120 vegetative cells binds to three adjacent sites in the xisA upstream region.

    PubMed Central

    Chastain, C J; Brusca, J S; Ramasubramanian, T S; Wei, T F; Golden, J W

    1990-01-01

    A DNA-binding factor (VF1) partially purified from Anabaena sp. strain PCC 7120 vegetative cell extracts by heparin-Sepharose chromatography was found to have affinity for the xisA upstream region. The xisA gene is required for excision of an 11-kilobase element from the nifD gene during heterocyst differentiation. Previous studies of the xisA upstream sequences demonstrated that deletion of this region is required for the expression of xisA from heterologous promoters in vegetative cells. Mobility shift assays with a labeled 250-base-pair fragment containing the binding sites revealed three distinct DNA-protein complexes. Competition experiments showed that VF1 also bound to the upstream sequences of the rbcL and glnA genes, but the rbcL and glnA fragments showed only single complexes in mobility shift assays. The upstream region of the nifH gene formed a weak complex with VF1. DNase footprinting and deletion analysis of the xisA binding site mapped the binding to a 66-base-pair region containing three repeats of the consensus recognition sequence ACATT. Images PMID:2118506

  17. Physical and chemical control of released microorganisms at field sites

    SciTech Connect

    Donegan, K.; Seidler, R.; Matyac, C.

    1991-01-01

    An important consideration in the environmental release of a genetically engineered microorganism (GEM) is the capability for reduction or elimination of GEM populations once their function is completed or if adverse environmental effects are observed. The decontamination treatments of burning and biocide application, alone and in combination with tilling, were evaluated for their ability to reduce populations of bacteria released on the phylloplane. Field plots of bush beans sprayed with the bacterium Erwinia herbicola, received the following treatments: (1) control, (2) control + till, (3) burn, (4) burn + till, (5) Kocide (cupric hydroxide), (6) Kocide + till, (7) Agri-strep (streptomycin sulfate), and (8) Agri-strept + till. Leaves and soil from the plots were sampled -1, 1, 5, 8, 12, 15, 19, and 27 days after application of the decontamination treatments. Burning produced a significant and persistent reduction in the number of bacteria whereas tilling, alone or in combination with the biocide treatments, stimulated a significant and persistent reduction in the number of bacteria, whereas tilling, alone or in combination with the biocide treatments, stimulated a significant increase in bacterial populations that persisted for several weeks.

  18. 10 CFR 960.5-2-2 - Site ownership and control.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Site ownership and control. 960.5-2-2 Section 960.5-2-2 Energy DEPARTMENT OF ENERGY GENERAL GUIDELINES FOR THE PRELIMINARY SCREENING OF POTENTIAL SITES FOR A..., in accordance with the requirements of 10 CFR 60.121, ownership, surface and subsurface rights,...

  19. 10 CFR 960.5-2-2 - Site ownership and control.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Site ownership and control. 960.5-2-2 Section 960.5-2-2 Energy DEPARTMENT OF ENERGY GENERAL GUIDELINES FOR THE PRELIMINARY SCREENING OF POTENTIAL SITES FOR A..., in accordance with the requirements of 10 CFR 60.121, ownership, surface and subsurface rights,...

  20. 10 CFR 960.5-2-2 - Site ownership and control.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Site ownership and control. 960.5-2-2 Section 960.5-2-2 Energy DEPARTMENT OF ENERGY GENERAL GUIDELINES FOR THE PRELIMINARY SCREENING OF POTENTIAL SITES FOR A..., in accordance with the requirements of 10 CFR 60.121, ownership, surface and subsurface rights,...

  1. Isolated metal active site concentration and stability control catalytic CO2 reduction selectivity.

    PubMed

    Matsubu, John C; Yang, Vanessa N; Christopher, Phillip

    2015-03-01

    CO2 reduction by H2 on heterogeneous catalysts is an important class of reactions that has been studied for decades. However, atomic scale details of structure-function relationships are still poorly understood. Particularly, it has been suggested that metal particle size plays a unique role in controlling the stability of CO2 hydrogenation catalysts and the distribution of active sites, which dictates reactivity and selectivity. These studies often have not considered the possible role of isolated metal active sites in the observed dependences. Here, we utilize probe molecule diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) with known site-specific extinction coefficients to quantify the fraction of Rh sites residing as atomically dispersed isolated sites (Rhiso), as well as Rh sites on the surface of Rh nanoparticles (RhNP) for a series of TiO2 supported Rh catalysts. Strong correlations were observed between the catalytic reverse water gas shift turn over frequency (TOF) and the fraction of Rhiso sites and between catalytic methanation TOF and the fraction of RhNP sites. Furthermore, it was observed that reaction condition-induced disintegration of Rh nanoparticles, forming Rhiso active sites, controls the changing reactivity with time on stream. This work demonstrates that isolated atoms and nanoparticles of the same metal on the same support can exhibit uniquely different catalytic selectivity in competing parallel reaction pathways and that disintegration of nanoparticles under reaction conditions can play a significant role in controlling stability. PMID:25671686

  2. A site-specific controlled-release system for metformin.

    PubMed

    Di Colo, Giacomo; Zambito, Ylenia; Baggiani, Andrea; Carelli, Vera; Serafini, Maria Francesca

    2005-05-01

    Oral absorption of the antihyperglycaemic agent metformin hydrochloride (MF-HCl) is confined to the upper part of the intestine, therefore rational controlled-release formulations of this drug should ensure a complete release during transit from stomach to jejunum. The aim of this study was the preparation of a system able to sustain release of high MF-HCl doses in compliance with the above requirement. Matrices (6 mm diameter; 50 mg weight) comprising varying drug-Precirol ATO 5 ratios were prepared by compression. The matrix containing 70% drug was coated on one face with Eudragit L100-55. Drug release to simulated gastric (SGF), jejunal (SJF) and ileal (SIF) fluids in sequence was studied using a modified USP rotating basket method. Release depended on drug load whereas it was independent of dissolution medium pH and hydrodynamics. Release kinetics were of radical t type and were determined by drug diffusion in aqueous pores created in the matrix by drug dissolution. An equation correlating rate-determining factors was developed, whereby the release pattern could be optimized. The half-coated matrix started release in SGF and completed it in SJF. The half-coated matrix, synchronizing drug release and matrix transit across the small intestine, may improve drug bioavailability and reduce side effects. PMID:15901345

  3. An analysis of two island groups as potential sites for trials of transgenic mosquitoes for malaria control

    PubMed Central

    Marsden, Clare D; Cornel, Anthony; Lee, Yoosook; Sanford, Michelle R; Norris, Laura C; Goodell, Parker B; Nieman, Catelyn C; Han, Sarah; Rodrigues, Amabelia; Denis, Joao; Ouledi, Ahmed; Lanzaro, Gregory C

    2013-01-01

    Considerable technological advances have been made towards the generation of genetically modified mosquitoes for vector control. In contrast, less progress has been made towards field evaluations of transformed mosquitoes which are critical for evaluating the success of, and hazards associated with, genetic modification. Oceanic islands have been highlighted as potentially the best locations for such trials. However, population genetic studies are necessary to verify isolation. Here, we used a panel of genetic markers to assess for evidence of genetic isolation of two oceanic island populations of the African malaria vector, Anopheles gambiae s.s. We found no evidence of isolation between the Bijagós archipelago and mainland Guinea-Bissau, despite separation by distances beyond the known dispersal capabilities of this taxon. Conversely, the Comoros Islands appear to be genetically isolated from the East African mainland, and thus represent a location worthy of further investigation for field trials. Based on assessments of gene flow within and between the Comoros islands, the island of Grande Comore was found to be genetically isolated from adjacent islands and also exhibited local population structure, indicating that it may be the most suitable site for trials with existing genetic modification technologies. PMID:23789035

  4. Crops with target-site herbicide resistance for Orobanche and Striga control.

    PubMed

    Gressel, Jonathan

    2009-05-01

    It is necessary to control root parasitic weeds before or as they attach to the crop. This can only be easily achieved chemically with herbicides that are systemic, or with herbicides that are active in soil. Long-term control can only be attained if the crops do not metabolise the herbicide, i.e. have target-site resistance. Such target-site resistances have allowed foliar applications of herbicides inhibiting enol-pyruvylshikimate phosphate synthase (EPSPS) (glyphosate), acetolactate synthase (ALS) (e.g. chlorsulfuron, imazapyr) and dihydropteroate synthase (asulam) for Orobanche control in experimental conditions with various crops. Large-scale use of imazapyr as a seed dressing of imidazolinone-resistant maize has been commercialised for Striga control. Crops with two target-site resistances will be more resilient to the evolution of resistance in the parasite, if well managed. PMID:19280593

  5. 43 CFR 420.3 - Adjacent lands.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 43 Public Lands: Interior 1 2014-10-01 2014-10-01 false Adjacent lands. 420.3 Section 420.3 Public Lands: Interior Regulations Relating to Public Lands BUREAU OF RECLAMATION, DEPARTMENT OF THE INTERIOR...-managing agencies on adjacent lands (both public and private)....

  6. 43 CFR 420.3 - Adjacent lands.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 43 Public Lands: Interior 1 2013-10-01 2013-10-01 false Adjacent lands. 420.3 Section 420.3 Public Lands: Interior Regulations Relating to Public Lands BUREAU OF RECLAMATION, DEPARTMENT OF THE INTERIOR...-managing agencies on adjacent lands (both public and private)....

  7. 43 CFR 420.3 - Adjacent lands.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 Public Lands: Interior 1 2011-10-01 2011-10-01 false Adjacent lands. 420.3 Section 420.3 Public Lands: Interior Regulations Relating to Public Lands BUREAU OF RECLAMATION, DEPARTMENT OF THE INTERIOR OFF-ROAD VEHICLE USE § 420.3 Adjacent lands. When administratively feasible, the regulation of off-road vehicle use on Reclamation lands will...

  8. 43 CFR 420.3 - Adjacent lands.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 43 Public Lands: Interior 1 2012-10-01 2011-10-01 true Adjacent lands. 420.3 Section 420.3 Public Lands: Interior Regulations Relating to Public Lands BUREAU OF RECLAMATION, DEPARTMENT OF THE INTERIOR...-managing agencies on adjacent lands (both public and private)....

  9. 43 CFR 420.3 - Adjacent lands.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Adjacent lands. 420.3 Section 420.3 Public Lands: Interior Regulations Relating to Public Lands BUREAU OF RECLAMATION, DEPARTMENT OF THE INTERIOR OFF-ROAD VEHICLE USE § 420.3 Adjacent lands. When administratively feasible, the regulation of...

  10. Engineering geomorphology at the cutting edge of land disturbance: erosion and sediment control on construction sites

    NASA Astrophysics Data System (ADS)

    Harbor, Jon

    1999-12-01

    Construction site management, traditionally dominated by professional engineers, provides an important opportunity for engineers and geomorphologists to work together in minimizing the environmental impacts of land disturbance. Areas disturbed for construction activity have soil erosion rates from 2 to 40,000 times greater than pre-construction conditions, and are important components of nonpoint source (NPS) pollution that degrades surface water quality. Despite significant local-to-watershed-scale environmental and economic impacts, the lack of an individual economic incentive for land developers to control erosion has limited voluntary adoption of erosion and sediment control measures. However, increased regulatory requirements, combined with efforts to identify and publicize the benefits of erosion control, are increasing the number of construction sites on which erosion control efforts are being implemented. Geomorphologists have the opportunity to play an active role in erosion and sediment control by implementing knowledge of erosion and sedimentation processes and of the variables that effect these processes. Pre-project geomorphological site assessments allow project designers to work around areas with high erosion potential, and to stage and schedule land disturbing activities to minimize erosion potential. Combined engineering and geomorphological analyses can increase the likelihood that streams and drainage channels are stable under altered hydrologic conditions, both during and after land use change, and can be used to design a drainage plan that minimizes surface water flow through disturbed areas. A variety of temporary measures to reduce erosion and to trap sediment on site can be designed and implemented, such as temporary surface covers, silt fence, and sedimentation basins. However, design and implementation of these measures require an understanding of erosion and sedimentation processes, and in many cases incorrect installation and maintenance

  11. Optical spectroscopy of site-controlled quantum dots in a Schottky diode

    NASA Astrophysics Data System (ADS)

    Yang, Lily; Carter, Samuel G.; Bracker, Allan S.; Yakes, Michael K.; Kim, Mijin; Kim, Chul Soo; Vora, Patrick M.; Gammon, Daniel

    2016-06-01

    The optical quality of site-controlled quantum dots is typically assessed by off-resonant photoluminescence spectroscopy, and emission linewidth is the most common figure of merit. Here, we combine photoluminescence and resonance fluorescence spectroscopy to obtain a more complete picture of site-controlled quantum dots embedded in a charge injection device. Although resonant and non-resonant linewidths are nearly as small as those of randomly nucleated dots, other optical properties show clear evidence of influence from defects introduced by the nanofabrication process. We demonstrate optical spin pumping and spin-flip Raman processes, which are important functions for use in quantum information applications.

  12. Identification of clustered YY1 binding sites in Imprinting Control Regions

    SciTech Connect

    Kim, J D; Hinz, A; Bergmann, A; Huang, J; Ovcharenko, I; Stubbs, L; Kim, J

    2006-04-19

    Mammalian genomic imprinting is regulated by Imprinting Control Regions (ICRs) that are usually associated with tandem arrays of transcription factor binding sites. In the current study, the sequence features derived from a tandem array of YY1 binding sites of Peg3-DMR (differentially methylated region) led us to identify three additional clustered YY1 binding sites, which are also localized within the DMRs of Xist, Tsix, and Nespas. These regions have been shown to play a critical role as ICRs for the regulation of surrounding genes. These ICRs have maintained a tandem array of YY1 binding sites during mammalian evolution. The in vivo binding of YY1 to these regions is allele-specific and only to the unmethylated active alleles. Promoter/enhancer assays suggest that a tandem array of YY1 binding sites function as a potential orientation-dependent enhancer. Insulator assays revealed that the enhancer-blocking activity is detected only in the YY1 binding sites of Peg3-DMR but not in the YY1 binding sites of other DMRs. Overall, our identification of three additional clustered YY1 binding sites in imprinted domains suggests a significant role for YY1 in mammalian genomic imprinting.

  13. Methane emissions from process equipment at natural gas production sites in the United States: pneumatic controllers.

    PubMed

    Allen, David T; Pacsi, Adam P; Sullivan, David W; Zavala-Araiza, Daniel; Harrison, Matthew; Keen, Kindal; Fraser, Matthew P; Daniel Hill, A; Sawyer, Robert F; Seinfeld, John H

    2015-01-01

    Emissions from 377 gas actuated (pneumatic) controllers were measured at natural gas production sites and a small number of oil production sites, throughout the United States. A small subset of the devices (19%), with whole gas emission rates in excess of 6 standard cubic feet per hour (scf/h), accounted for 95% of emissions. More than half of the controllers recorded emissions of 0.001 scf/h or less during 15 min of measurement. Pneumatic controllers in level control applications on separators and in compressor applications had higher emission rates than controllers in other types of applications. Regional differences in emissions were observed, with the lowest emissions measured in the Rocky Mountains and the highest emissions in the Gulf Coast. Average methane emissions per controller reported in this work are 17% higher than the average emissions per controller in the 2012 EPA greenhouse gas national emission inventory (2012 GHG NEI, released in 2014); the average of 2.7 controllers per well observed in this work is higher than the 1.0 controllers per well reported in the 2012 GHG NEI. PMID:25488196

  14. Site-controlled InGaN/GaN single-photon-emitting diode

    NASA Astrophysics Data System (ADS)

    Zhang, Lei; Teng, Chu-Hsiang; Ku, Pei-Cheng; Deng, Hui

    2016-04-01

    We report single-photon emission from electrically driven site-controlled InGaN/GaN quantum dots. The device is fabricated from a planar light-emitting diode structure containing a single InGaN quantum well, using a top-down approach. The location, dimension, and height of each single-photon-emitting diode are controlled lithographically, providing great flexibility for chip-scale integration.

  15. Design and control strategy for a hybrid green energy system for mobile telecommunication sites

    NASA Astrophysics Data System (ADS)

    Okundamiya, Michael S.; Emagbetere, Joy O.; Ogujor, Emmanuel A.

    2014-07-01

    The rising energy costs and carbon footprint of operating mobile telecommunication sites in the emerging world have increased research interests in green technology. The intermittent nature of most green energy sources creates the problem of designing the optimum configuration for a given location. This study presents the design analysis and control strategy for a cost effective and reliable operation of the hybrid green energy system (HGES) for GSM base transceiver station (BTS) sites in isolated regions. The design constrains the generation and distribution of power to reliably satisfy the energy demand while ensuring safe operation of the system. The overall process control applies the genetic algorithm-based technique for optimal techno-economic sizing of system's components. The process simulation utilized meteorological data for 3 locations (Abuja, Benin City and Sokoto) with varying climatic conditions in Nigeria. Simulation results presented for green GSM BTS sites are discussed and compared with existing approaches.

  16. 76 FR 81986 - Honeywell International, Inc., Automation and Control Solutions Division, Including On-Site...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-29

    ..., 2010 (75 FR 49531). The notice was amended on December 7, 2010 to include several on-site leased worker firms. The amended notice was published in the Federal Register on December 13, 2010 (75 FR 77664-77665... Employment and Training Administration Honeywell International, Inc., Automation and Control...

  17. Integrated Weed Control for Land Stewardship at Legacy Management's Rocky Flats Site in Colorado - 13086

    SciTech Connect

    Nelson, Jody K.

    2013-07-01

    Land stewardship is one of nine sustainability programs in the U.S. Department of Energy's Environmental Management System. Land stewardship includes maintaining and improving ecosystem health. At the Rocky Flats Site near Westminster, Colorado, land stewardship is an integral component of the Office of Legacy Management's post-closure monitoring and management at the site. Nearly 263 hectares (650 acres) were disturbed and re-vegetated during site cleanup and closure operations. Proactive management of revegetation areas is critical to the successful reestablishment of native grasslands, wetlands, and riparian communities. The undisturbed native plant communities that occur at the site also require active management to maintain the high-quality wetlands and other habitats that are home to numerous species of birds and other wildlife such as elk and deer, rare plant communities, and the federally listed threatened Preble's meadow jumping mouse. Over the past several decades, an increase of Noxious weeds has impacted much of Colorado's Front Range. As a result, weed control is a key component of the land stewardship program at Rocky Flats. Thirty-three species of state-listed Noxious weeds are known to occur in the Central and Peripheral Operable Units at Rocky Flats, along with another five species that are considered invasive at the site. Early detection and rapid response to control new invasive species is crucial to the program. An integrated weed control/vegetation management approach is key to maintaining healthy, sustainable plant communities that are able to resist Noxious weed invasions. Weed mapping, field surveys, and field-staff training sessions (to learn how to identify new potential problem species) are conducted to help detect and prevent new weed problems. The integrated approach at Rocky Flats includes administrative and cultural techniques (prevention), mechanical controls, biological controls, and chemical controls. Several species of biocontrol

  18. Drivers of tall shrub proliferation adjacent to the Dempster Highway, Northwest Territories, Canada

    NASA Astrophysics Data System (ADS)

    Cameron, Emily A.; Lantz, Trevor C.

    2016-04-01

    Arctic ecosystems are undergoing rapid changes as a result of climate warming and more frequent disturbances. Disturbances can have particularly large effects on high-latitude ecosystems when ecosystem structure and function is controlled by strong feedbacks between soil conditions, vegetation, and ground thermal regime. In this study we investigated the impact of road construction and maintenance on vegetation structure and biomass along the Dempster Highway where it crosses the Peel Plateau in the Northwest Territories. To explore drivers of tall shrub proliferation and to quantify shrub proliferation in this region of continuous permafrost, greyscale air photos (1975) and Quickbird satellite imagery (2008) were used to map landcover change within two 0.6 km2 belts next to the road and two 0.6 km2 belts 500 m away from the road. Maps showing areas where: 1) tall shrubs expanded, and 2) dwarf shrub tundra resisted invasion were then used to select field sites where a suite of biophysical variables were measured. Rapid tall shrub proliferation and greater biomass adjacent to the road indicate that disturbance can facilitate vegetation change in tundra environments. Our field data also suggests that increased shrub proliferation adjacent to the road was caused by greater soil moisture. Tall shrub proliferation adjacent to the road occurred at lower elevation sites characterized by wetter soils with thicker organic layers. Areas that resisted tall shrub encroachment were located at higher elevations and had drier soils with thin organic layers. Our observations also support previous work illustrating that tall shrub expansion next to the highway promotes strong positive feedbacks to ongoing shrub growth and proliferation.

  19. Controlling Site Selectivity in Palladium-Catalyzed C–H Bond Functionalization

    PubMed Central

    Neufeldt, Sharon R.; Sanford, Melanie S.

    2012-01-01

    Conspectus Effective methodology to functionalize C–H bonds requires overcoming the key challenge of differentiating among the multitude of C–H bonds that are present in complex organic molecules. This Account focuses on our work over the past decade toward the development of site-selective Pd-catalyzed C–H functionalization reactions using the following approaches: substrate-based control over selectivity through the use of directing groups (approach 1), substrate control through the use of electronically activated substrates (approach 2), or catalyst-based control (approach 3). In our extensive exploration of the first approach, a number of selectivity trends have emerged for both sp2 and sp3 C–H functionalization reactions that hold true for a variety of transformations involving diverse directing groups. Functionalizations tend to occur at the less-hindered sp2 C–H bond ortho to a directing group, at primary sp3 C–H bonds that are β to a directing group, and, when multiple directing groups are present, at C–H sites proximal to the most basic directing group. Using approach 2, which exploits electronic biases within a substrate, our group has achieved C-2-selective arylation of indoles and pyrroles using diaryliodonium oxidants. The selectivity of these transformations is altered when the C-2 site of the heterocycle is blocked, leading to C–C bond formation at the C-3 position. While approach 3 (catalyst-based control) is still in its early stages of exploration, we have obtained exciting results demonstrating that site selectivity can be tuned by modifying the structure of the supporting ligands on the Pd catalyst. For example, by modulating the structure of N~N bidentate ligands, we have achieved exquisite levels of selectivity for arylation at the α site of naphthalene. Similarly, we have demonstrated that both the rate and site selectivity of arene acetoxylation depend on the ratio of pyridine (ligand) to Pd. Lastly, by switching the ligand

  20. Corrosion Control Measures For Liquid Radioactive Waste Storage Tanks At The Savannah River Site

    SciTech Connect

    Wiersma, B. J.; Subramanian, K. H.

    2012-11-27

    The Savannah River Site has stored radioactive wastes in large, underground, carbon steel tanks for approximately 60 years. An assessment of potential degradation mechanisms determined that the tanks may be vulnerable to nitrate- induced pitting corrosion and stress corrosion cracking. Controls on the solution chemistry and temperature of the wastes are in place to mitigate these mechanisms. These controls are based upon a series of experiments performed using simulated solutions on materials used for construction of the tanks. The technical bases and evolution of these controls is presented in this paper.

  1. Single photon emission from site-controlled InGaN/GaN quantum dots

    SciTech Connect

    Zhang, Lei; Hill, Tyler A.; Deng, Hui; Teng, Chu-Hsiang; Lee, Leung-Kway; Ku, Pei-Cheng

    2013-11-04

    Single photon emission was observed from site-controlled InGaN/GaN quantum dots. The single-photon nature of the emission was verified by the second-order correlation function up to 90 K, the highest temperature to date for site-controlled quantum dots. Micro-photoluminescence study on individual quantum dots showed linearly polarized single exciton emission with a lifetime of a few nanoseconds. The dimensions of these quantum dots were well controlled to the precision of state-of-the-art fabrication technologies, as reflected in the uniformity of their optical properties. The yield of optically active quantum dots was greater than 90%, among which 13%–25% exhibited single photon emission at 10 K.

  2. The Healthy Worker Project: a work-site intervention for weight control and smoking cessation.

    PubMed Central

    Jeffery, R W; Forster, J L; French, S A; Kelder, S H; Lando, H A; McGovern, P G; Jacobs, D R; Baxter, J E

    1993-01-01

    OBJECTIVES. A randomized trial was conducted to evaluate the effectiveness of a work-site health promotion program in reducing obesity and the prevalence of cigarette smoking. METHODS. Thirty-two work sites were randomized to treatment or no treatment for 2 years. Treatment consisted of health education classes combined with a payroll-based incentive system. Evaluation was based on cohort and cross-sectional surveys. RESULTS. Of 10,000 total employees in treatment work sites, 2041 and 270 participated in weight control and smoking cessation programs, respectively. Weight losses averaged 4.8 lbs, and 43% of smoking participants quit. Net 2-year reductions in smoking prevalence in treatment vs control work sites were 4.0% and 2.1% in cross-sectional and cohort surveys, respectively. No treatment effect was found for weight. Treatment effects for smoking prevalence and weight were both positively correlated with participation rates in the intervention programs (r = .45 for smoking and r = .55 for weight). CONCLUSIONS. This work-site health promotion program was effective in reducing smoking prevalence at a cost that is believed to make the investment worthwhile. PMID:8438979

  3. The control of acid mine drainage at the Summitville Mine Superfund Site

    SciTech Connect

    Ketellapper, V.L.; Williams, L.O.

    1996-11-01

    The Summitville Mine Superfund Site is located about 25 miles south of Del Norte, Colorado, in Rio Grande County. Occurring at an average elevation of 11,500 feet in the San Juan Mountain Range, the mine site is located two miles east of the Continental Divide. Mining at Summitville has occurred since 1870. The mine was most recently operated by Summitville Consolidated Mining Company, Inc. (SCMCI) as an open pit gold mine with extraction by means of a cyanide leaching process. In December of 1992, SCMCI declared bankruptcy and vacated the mine site. At that time, the US Environmental Protection Agency (EPA) took over operations of the water treatment facilities to prevent a catastrophic release of cyanide and metal-laden water from the mine site. Due to high operational costs of water treatment (approximately $50,000 per day), EPA established a goal to minimize active water treatment by reducing or eliminating acid mine drainage (AMD). All of the sources of AMD generation on the mine site were evaluated and prioritized. Of the twelve areas identified as sources of AMD, the Cropsy Waste Pile, the Summitville Dam Impoundment, the Beaver Mud Dump, the Reynolds and Chandler adits, and the Mine Pits were consider to be the most significant contributors to the generation of metal-laden acidic (low pH) water. A two part plan was developed to control AMD from the most significant sources. The first part was initiated immediately to control AMD being released from the Site. This part focused on improving the efficiency of the water treatment facilities and controlling the AMD discharges from the mine drainage adits. The second part of the plan was aimed at reducing the AMD generated in groundwater and surface water runoff from the mine wastes. A lined and capped repository located in the mine pits for acid generating mining waste and water treatment plant sludge was found to be the most feasible alternative.

  4. Radiological control FY 1995 site support program plan WBS 6.7.2.4

    SciTech Connect

    Not Available

    1994-09-01

    The 1995 Site Support Program Plan (SSPP) brings year planning and execution year planning into a single document. The plan presented consists of the following four major sections: Overview and Introduction - Health physics has been renamed Radiological Control (RadCon) with the role of protecting workers, the public and the environment from the harmful effects of radiation resulting from the DOE Hanford Site Operations; Cost Baselines which contains cost, technical and schedule baselines; Execution Year work Plan - cost summaries and detailed descriptions of the work to be done; Appendix - including brief description of other project activities directly coupled to RadCon.

  5. On the time-course of adjacent and non-adjacent transposed-letter priming

    PubMed Central

    Ktori, Maria; Kingma, Brechtsje; Hannagan, Thomas; Holcomb, Phillip J.; Grainger, Jonathan

    2014-01-01

    We compared effects of adjacent (e.g., atricle-ARTICLE) and non-adjacent (e.g., actirle-ARTICLE) transposed-letter (TL) primes in an ERP study using the sandwich priming technique. TL priming was measured relative to the standard double-substitution condition. We found significantly stronger priming effects for adjacent transpositions than non-adjacent transpositions (with 2 intervening letters) in behavioral responses (lexical decision latencies), and the adjacent priming effects emerged earlier in the ERP signal, at around 200 ms post-target onset. Non-adjacent priming effects emerged about 50 ms later and were short-lived, being significant only in the 250-300 ms time-window. Adjacent transpositions on the other hand continued to produce priming in the N400 time-window (300-500 ms post-target onset). This qualitatively different pattern of priming effects for adjacent and non-adjacent transpositions is discussed in the light of different accounts of letter transposition effects, and the utility of drawing a distinction between positional flexibility and positional noise. PMID:25364497

  6. The independent relationship between trouble controlling Facebook use, time spent on the site and distress

    PubMed Central

    Muench, Fredrick; Hayes, Marie; Kuerbis, Alexis; Shao, Sijing

    2015-01-01

    Background and Aims There is an emerging literature base on the relationship between maladaptive traits and “addiction” to social networking sites. These studies have operationalized addiction as either spending excessive amounts of time on social networking sites (SNS) or trouble controlling SNS use, but have not assessed the unique contribution of each of these constructs on outcomes in the same models. Moreover, these studies have exclusively been conducted with younger people rather than a heterogeneous sample. This study examined the independent relationship of a brief Facebook addiction scale, time spent on Facebook, and Facebook checking on positive and negative social domains, while controlling for self-esteem and social desirability. Methods Participants were recruited using e-mail, SNS posts and through Amazon’s MTurk system. The sample included 489 respondents ages from 18 to approximately 70, who completed a 10–15 minute survey. Results Results indicate that neither time spent on Facebook nor Facebook checking was significantly associated with either self-esteem, fear of negative social evaluation or social comparison, while SNS addiction symptoms were each independently associated with Facebook usage. Neither time spent on Facebook nor SNS addiction symptoms were associated with positive social relationships. Discussion Overall results suggest that time on SNS and trouble controlling use should be considered independent constructs and that interventions should target underlying loss of control as the primary intervention target above ego syntonic time spent on the site. PMID:26551906

  7. Binding of bovine thyrotropin to specific sites in thyroid tissue from control and hemithyroidectomized rats

    SciTech Connect

    Clark, O.H.; Lambert, W.R.; Amir, S.M.; Ingbar, S.H.

    1985-12-01

    The binding of 125I-bovine thyrotropin to thyroid particulate fractions from sham-operated (control) and hemithyroidectomized rats was compared to determine if a change in either the number of bovine thyroid-stimulating hormone (bTSH) binding sites or their affinity for bTSH occurs in physiological situations that evoke changes in the intensity of thyroid stimulation. Following hemithyroidectomy serum TSH levels increase and the remnant thyroid lobe enlarges. Because of compensatory thyroid hypertrophy the concentration of TSH binding sites in the thyroid glands from hemithyroidectomized and control rats was related to particulate protein concentration, to the degree of thyroid cellularity as indicated by DNA concentration, and to the concentration of the plasma membrane markers, 5'-nucleotidase and magnesium-dependent ATPase. In each of four experiments, saturation studies revealed that the maximum specific binding of TSH per unit particulate protein and per thyroid lobe was greater in particulates from remnant than from control thyroid lobes. When related to DNA concentration, the concentration of TSH binding sites in remnant lobes was approximately twice that in control lobes. Because of an increase in plasma membrane markers per lobe after hemithyroidectomy, however, there was no difference in the number of TSH binding sites when related to the concentrations of the membrane marker enzymes in the particulate fractions. As judged from Scatchard analysis, the affinity of TSH binding was lower in remnant than in control lobes. This was partially but not completely due to the increased concentration of particulate protein in the remnant thyroid. These experiments demonstrate that the increase in serum TSH levels after hemithyroidectomy in the rat is associated with alterations in TSH receptor capacity and affinity.

  8. Air strippers and their emissions control at Superfund sites. Technical report, February-April 1987

    SciTech Connect

    Blaney, B.L.; Branscome, M.

    1988-08-01

    Air stripping, a traditional means of making slightly contaminated ground water potable, is being applied increasingly to more-severe groundwater pollution at remedial action sites. Concentrations of volatile and semivolatile compounds at such sites may reach hundreds of parts per million. As a result, several changes have resulted in air-stripping technology. New air stripping technologies are being employed to achieve very high (>99% removal of volatile compounds and to increase the removal of semivolatiles. New stripper designs are being investigated for compactness and mobility. In addition, emissions controls are being added because air-pollution impacts are larger. The paper discusses these trends and provides examples from ground-water cleanup at remedial-action sites in the United States.

  9. Radiotherapy is Important for Local Control at Primary and Metastatic Sites in Pediatric Rhabdomyosarcoma

    PubMed Central

    Abish, Sharon; Mitchell, David; Freeman, Carolyn

    2015-01-01

    Purpose: The current recommended practice for pediatric patients with metastatic rhabdomyosarcoma includes full-dose radiotherapy to each metastatic site. We wished to question this practice, which can cause side-effects and is often logistically challenging, by studying the pattern of failure in our pediatric and teenage patient population. Methods and Materials: Our institution’s cancer registry was queried for patients diagnosed with rhabdomyosarcoma aged 18 or less from January 1990 until January 2014. Twenty-nine patients were found and, of these, six had metastatic disease. Five of the six were treated with standard chemotherapy together with radiotherapy to the primary and metastatic sites with doses and fractionation according to the site. Progression-free survival was calculated from the end of radiotherapy until radiological or pathological evidence of disease progression or death. Results: Median age was 13 years (range: 12-18). Three were girls. All had alveolar histology and unfavorable primary sites. Twelve metastatic sites were treated with radiotherapy. Doses used were 41.4 - 50.4 Gy in 1.8 Gy fractions for most sites, and 15 Gy in 1.5 Gy fractions for whole lung radiotherapy. The median number of sites treated per patient was two (range: 1 - 6). Median time to progression was 10.1 months (range: 1.9 - 15.7). Local control was 100% for all metastatic sites. Median overall survival (OS) was 31.8 months (range: 20.4 – 95.4 months). Three patients developed progressive disease outside the treated field. One patient died from a secondary hematological malignancy without evidence of disease progression. One patient remains progression-free at 88.6 months post-radiotherapy. Conclusions: Radiotherapy to metastatic disease sites prevented in-field progression in all five patients with metastatic alveolar rhabdomyosarcoma. However, failure at sites outside of the radiotherapy volume occurred in three of five of patients and overall survival was very

  10. ENHANCED CONTROL OF MERCURY BY WET FLUE GAS DESULFURIZATION SYSTEMS--SITE 2 RESULTS

    SciTech Connect

    G. Blythe; S. Miller; C. Richardson; K. Searcy

    2000-02-01

    The U.S. Department of Energy and EPRI are co-funding this project to improve the control of mercury emissions from coal-fired power plants equipped with wet flue gas desulfurization (FGD) systems. The project is investigating catalytic oxidation of vapor-phase elemental mercury to a form that is more effectively captured in wet FGD systems. If successfully developed, the process could be applicable to over 90,000 MW of utility generating capacity with existing FGD systems, and to future FGD installations. Field tests are being conducted to determine whether candidate catalyst materials remain active towards mercury oxidation after extended flue gas exposure. Catalyst life will have a large impact on the cost effectiveness of this potential process. A mobile catalyst test unit is being used to test the activity of four different catalysts for a period of up to six months at each of three utility sites. Catalyst testing at the first site, which fires Texas lignite, was completed in December 1998. Testing at the second test site, which fires a Powder River Basin subbituminous coal, was completed in the fall of 1999, and testing at the third site, which fires a high-sulfur bituminous coal, will begin in early 2000. This technical note reports results from Site 2; results from Site 1 were reported in a previous technical note. At Site 2, catalysts were tested in several forms, including powders dispersed in sand bed reactors and in more commercially viable forms such as extruded beads and coated honeycomb structures. This technical note presents results from Site 2 for both the sand bed reactors and commercial catalyst forms. Site 3 results are not yet available, but should be available late in the year 2000. Field testing is being supported by laboratory tests to screen catalysts for activity at specific flue gas compositions, to investigate catalyst deactivation mechanisms and to investigate methods for regenerating spent catalysts. Laboratory results related to the

  11. Small mammal-heavy metal concentrations from mined and control sites

    USGS Publications Warehouse

    Smith, G.J.; Rongstad, O.J.

    1982-01-01

    Total body concentrations of zinc, copper, cadmium, lead, nickel, mercury and arsenic were determined for Peromyscus maniculatus and Microtus pennsylvanicus from an active zinc-copper mine near Timmins, Ontario, Canada, and a proposed zinc-copper mine near Crandon, Wisconsin, USA. Metal concentrations were evaluated with respect to area, species, sex and age groups. Metal concentrations in Peromyscus from the proposed mine site were not different from those collected in a third area where no mine or deposit exists. This is probably due to the 30 m of glacial material over the proposed mine site deposit. A statistical interaction between area, species, sex and age was observed for zinc and copper concentrations in small mammals we examined. Peromyscus from the mine site had consistently higher metal concentrations than Peromyscus from the control site. Greater total body cadmium and lead concentrations in adult?compared with juvenile?Peromyscus collected at the mine site suggests age-dependent accumulation of these toxic metals. Microtus did not exhibit this age-related response, and responded to other environmental metals more erratically and to a lesser degree. Differences in the response of these two species to environmental metal exposure may be due to differences in food habits. Nickel, mercury and arsenic concentrations in small mammals from the mine site were not different from controls. Heavy metal concentrations are also presented for Sorex cinereus, Blarina brevicauda and Zapus hudsonicus without respect to age and sex cohorts. Peromyscus may be a potentially important species for the monitoring of heavy metal pollution.

  12. Exposure to crystalline silica at Alberta work sites: review of controls.

    PubMed

    Radnoff, Diane; Todor, Maria S; Beach, Jeremy

    2015-01-01

    From 2009 to 2013, Alberta Jobs, Skills, Training, and Labour (JSTL) conducted a project to evaluate exposure to crystalline silica and assess controls to protect workers. Information on exposure results has been previously reported; this article discusses the data collected on workplace controls. Information on work site controls was collected during exposure assessments consisting of qualitative information on controls in place and used by workers at the time of the assessments. Where there was sufficient data, the information was further analyzed to evaluate the impact of a particular control. While many types of controls were observed, they were not always effective or in use. The control available most often was respiratory protective equipment (RPE). Generally, when respirators were used, they were correctly selected for the level of measured exposure. However, not all workers who were potentially overexposed wore respirators at the time of the assessments. When the use of respirators was taken into account, about one-third of workers were still potentially exposed over the Alberta occupational exposure limit. The industries with the highest levels of exposure tended to be those with the most unprotected workers. Issues were identified with the use of improper work practices such as dry cleaning methods, lack of documented work procedures, poor housekeeping, and lack of training which may have contributed to worker exposure levels. There is a wide range in the efficacy of controls, particularly engineering controls. Most of the literature focuses on engineering controls; however administrative controls also play a role in reducing worker exposure. Data collected in this work indicated that simple changes to work procedures and behavior (such as improved housekeeping) may be effective, low-cost ways to reduce workplace exposure. More study is required to evaluate the impact and efficacy of administrative controls such as housekeeping and training. Employers

  13. Impact of adjacent land use on coastal wetland sediments.

    PubMed

    Karstens, Svenja; Buczko, Uwe; Jurasinski, Gerald; Peticzka, Robert; Glatzel, Stephan

    2016-04-15

    Coastal wetlands link terrestrial with marine ecosystems and are influenced from both land and sea. Therefore, they are ecotones with strong biogeochemical gradients. We analyzed sediment characteristics including macronutrients (C, N, P, K, Mg, Ca, S) and heavy metals (Mn, Fe, Cu, Zn, Al, Co, Cr, Ni) of two coastal wetlands dominated by Phragmites australis at the Darss-Zingst Bodden Chain, a lagoon system at the Southern Baltic Sea, to identify the impact of adjacent land use and to distinguish between influences from land or sea. In the wetland directly adjacent to cropland (study site Dabitz) heavy metal concentrations were significantly elevated. Fertilizer application led to heavy metal accumulation in the sediments of the adjacent wetland zones. In contrast, at the other study site (Michaelsdorf), where the hinterland has been used as pasture, heavy metal concentrations were low. While the amount of macronutrients was also influenced by vegetation characteristics (e.g. carbon) or water chemistry (e.g. sulfate), the accumulation of heavy metals is regarded as purely anthropogenic influence. A principal component analysis (PCA) based on the sediment data showed that the wetland fringes of the two study sites are not distinguishable, neither in their macronutrient status nor in their concentrations of heavy metals, whereas the interior zones exhibit large differences in terms of heavy metal concentrations. This suggests that seaside influences are minor compared to influences from land. Altogether, heavy metal concentrations were still below national precautionary and action values. However, if we regard the macronutrient and heavy metal concentrations in the wetland fringes as the natural background values, an accumulation of trace elements from agricultural production in the hinterland is apparent. Thus, coastal wetlands bordering croplands may function as effective pollutant buffers today, but the future development has to be monitored closely to avoid

  14. An Evaluation of Techniques to Control Problem Bird Species on Landfill Sites

    NASA Astrophysics Data System (ADS)

    Cook, Aonghais; Rushton, Steven; Allan, John; Baxter, Andrew

    2008-06-01

    Birds feeding on landfill sites cause problems in terms of nuisance to neighbors, flight safety, a threat to public health, and affecting the day to day site operation. A number of control measures exist to deter problem species; however, research into their effectiveness across sites and for multiple species has been limited. We use a modeling approach in order to assess the effectiveness of nine techniques — pyrotechnics, hand-held distress calls, static distress calls, blank ammunition, a combination of blank and lethal use of ammunition, the use of falcons, the use of hawks, wailers and helium-filled bird-scaring kites — at deterring three commonly recorded species — the Black-headed Gull ( Larus ridibundus), the Herring Gull ( Larus argentatus) and the Lesser Black-backed Gull ( Larus fuscus) — from six landfill sites across the United Kingdom. The use of distress calls, falconry, and combinations of lethal and nonlethal use of ammunition were the most effective techniques for initially deterring birds from these sites. However, when habituation is considered, there is a clear difference between techniques such as falconry, which have a lethal aspect and may act to reinforce the deterrence, and the use of techniques such as distress calls, which do not. However there are problems related to legislation and public perception when lethal techniques are used.

  15. An evaluation of techniques to control problem bird species on landfill sites.

    PubMed

    Cook, Aonghais; Rushton, Steven; Allan, John; Baxter, Andrew

    2008-06-01

    Birds feeding on landfill sites cause problems in terms of nuisance to neighbors, flight safety, a threat to public health, and affecting the day to day site operation. A number of control measures exist to deter problem species; however, research into their effectiveness across sites and for multiple species has been limited. We use a modeling approach in order to assess the effectiveness of nine techniques--pyrotechnics, hand-held distress calls, static distress calls, blank ammunition, a combination of blank and lethal use of ammunition, the use of falcons, the use of hawks, wailers and helium-filled bird-scaring kites --at deterring three commonly recorded species--the Black-headed Gull (Larus ridibundus), the Herring Gull (Larus argentatus) and the Lesser Black-backed Gull (Larus fuscus)--from six landfill sites across the United Kingdom. The use of distress calls, falconry, and combinations of lethal and nonlethal use of ammunition were the most effective techniques for initially deterring birds from these sites. However, when habituation is considered, there is a clear difference between techniques such as falconry, which have a lethal aspect and may act to reinforce the deterrence, and the use of techniques such as distress calls, which do not. However there are problems related to legislation and public perception when lethal techniques are used. PMID:18256779

  16. Sites of Regulated Phosphorylation that Control K-Cl Cotransporter Activity

    PubMed Central

    Rinehart, Jesse; Maksimova, Yelena D.; Tanis, Jessica E.; Stone, Kathryn L.; Hodson, Caleb A.; Zhang, Junhui; Risinger, Mary; Pan, Weijun; Wu, Dianqing; Colangelo, Christopher M.; Forbush, Biff; Joiner, Clinton H.; Gulcicek, Erol E.; Gallagher, Patrick G.; Lifton, Richard P.

    2010-01-01

    Summary Modulation of intracellular chloride concentration ([Cl−]i) plays a fundamental role in cell volume regulation and neuronal response to GABA. Cl− exit via K-Cl cotransporters (KCCs) is a major determinant of [Cl−]I; however, mechanisms governing KCC activities are poorly understood. We identified two sites in KCC3 that are rapidly dephosphorylated in hypotonic conditions in cultured cells and human red blood cells in parallel with increased transport activity. Alanine substitutions at these sites result in constitutively active cotransport. These sites are highly phosphorylated in plasma membrane KCC3 in isotonic conditions, suggesting that dephosphorylation increases KCC3's intrinsic transport activity. Reduction of WNK1 expression via RNA interference reduces phosphorylation at these sites. Homologous sites are phosphorylated in all human KCCs. KCC2 is partially phosphorylated in neonatal mouse brain and dephosphorylated in parallel with KCC2 activation. These findings provide insight into regulation of [Cl−]i and have implications for control of cell volume and neuronal function. PMID:19665974

  17. Site-controlled quantum dots coupled to a photonic crystal molecule

    SciTech Connect

    Rigal, B.; Jarlov, C.; Gallo, P.; Dwir, B.; Rudra, A.; Calic, M.; Kapon, E.

    2015-10-05

    Two site-controlled quantum dots (QDs) were integrated in a photonic crystal molecule (PCM) formed by L3 nanocavities. A statistical analysis of the coupled cavity modes demonstrated the formation of bonding and anti-bonding delocalized PCM states. Excitonic transitions belonging to each QD were identified by scanning micro-photoluminescence spectroscopy. Co-polarization of the QDs photoluminescence with the coupled cavity modes provides evidence for the simultaneous coupling of two spatially separated QDs to the same PCM mode.

  18. Visible, Durable, Enforceable Institutional Controls: Weldon Spring Site - A 10-Year Journey - 13190

    SciTech Connect

    Uhlmeyer, Terri; Thompson, Randy; Starr, Ken

    2013-07-01

    The DOE Office of Legacy Management's (LM's) mission is to manage the DOE's post-closure responsibilities and ensure the future protection of human health and the environment. LM has control and custody of legacy land, structures, and facilities and is responsible for maintaining them at levels suitable for their long-term use. This includes all engineered and institutional controls (ICs) designed as another level of assurance to prevent exposure to residual contamination and waste. The development and management of ICs has been, and continues to be, a critical component to the success of LM surveillance and maintenance activities. Many major federal laws, Executive Orders, regulations, and various other drivers influence the establishment and use of ICs at LM sites. LM uses a wide range of ICs to appropriately limit access to, or uses of, land, facilities, and other real and personal properties; protect the environment; maintain the physical safety and security of DOE facilities; and prevent or limit inadvertent human and environmental exposure to residual contaminants and other hazards. The ICs at the Weldon Spring, Missouri, Site were developed in close coordination with federal and state regulators. An Explanation of Significant Differences (ESD) was issued in February 2005, which clarified the use restrictions necessary for the remedial actions specified in the Records of Decision for the separate operable units to remain protective over the long-term. The operable units included the Chemical Plant Operable Unit, the Chemical Plant Groundwater Operable Unit, and the Quarry Residuals Operable Unit. The ESD clarified specific requirements for each site area that needed use restrictions and established how DOE would implement, maintain, and monitor the specific requirements. DOE developed the Long-Term Surveillance and Maintenance Plan for the U.S. Department of Energy Weldon Spring, Missouri, Site (LTS and M Plan) that addressed the full scope of the site

  19. Site-controlled Ag nanocrystals grown by molecular beam epitaxy-Towards plasmonic integration technology

    SciTech Connect

    Urbanczyk, Adam; Noetzel, Richard

    2012-12-15

    We demonstrate site-controlled growth of epitaxial Ag nanocrystals on patterned GaAs substrates by molecular beam epitaxy with high degree of long-range uniformity. The alignment is based on lithographically defined holes in which position controlled InAs quantum dots are grown. The Ag nanocrystals self-align preferentially on top of the InAs quantum dots. No such ordering is observed in the absence of InAs quantum dots, proving that the ordering is strain-driven. The presented technique facilitates the placement of active plasmonic nanostructures at arbitrarily defined positions enabling their integration into complex devices and plasmonic circuits.

  20. ENHANCED CONTROL OF MERCURY BY WET FLUE GAS DESULFURIZATION SYSTEMS--SITE 2 RESULTS

    SciTech Connect

    G. Blythe; S. Miller; C. Richardson; K. Searcy

    2000-06-01

    The U.S. Department of Energy and EPRI are co-funding this project to improve the control of mercury emissions from coal-fired power plants equipped with wet flue gas desulfurization (FGD) systems. The project is investigating catalytic oxidation of vapor-phase elemental mercury to a form that is more effectively captured in wet FGD systems. If successfully developed, the process could be applicable to over 90,000 MW of utility generating capacity with existing FGD systems, and to future FGD installations. Field tests are being conducted to determine whether candidate catalyst materials remain active towards mercury oxidation after extended flue gas exposure. Catalyst life will have a large impact on the cost effectiveness of this potential process. A mobile catalyst test unit is being used to test the activity of four different catalysts for a period of up to six months at each of three utility sites. Catalyst testing at the first site, which fires Texas lignite, was completed in December 1998. Testing at the second test site, which fires a Powder River Basin subbituminous coal, was completed in the fall of 1999, and testing at the third site, which fires a high-sulfur bituminous coal, will begin in 2000. This technical note reports results from Site 2; results from Site 1 were reported in a previous technical note. At Site 2, catalysts were tested in several forms, including powders dispersed in sand bed reactors and in commercial forms such as extruded beads and coated honeycomb structures. This technical note presents results from Site 2 for both the sand bed reactors and commercial catalyst forms. Field testing is being supported by laboratory tests to screen catalysts for activity at specific flue gas compositions, to investigate catalyst deactivation mechanisms and to investigate methods for regenerating spent catalysts. Laboratory results related to the Site 2 field effort are also included and discussed in this technical note. Preliminary economics, based

  1. Stormwater Management Criteria for On-Site Pollution Control: A Comparative Assessment of International Practices

    NASA Astrophysics Data System (ADS)

    Sage, Jérémie; Berthier, Emmanuel; Gromaire, Marie-Christine

    2015-07-01

    Over the last decade, a growing interest has been shown toward innovative stormwater management practices, breaking away from conventional "end of pipe" approaches (based on conveying water offsite to centralized detention facilities). Innovative strategies, referred to as sustainable urban drainage systems, low impact development (LID) or green infrastructures, advocating for management of runoff as close to its origin as possible, have therefore gained a lot of popularity among practitioners and public authorities. However, while the need for pollution control is generally well accepted, there is no wide agreement about management criteria to be given to developers. This article hence aims to compare these criteria through literature analysis of different state or local stormwater management manuals or guidelines, investigating both their suitability for pollution control and their influence on best management practices selection and design. Four categories of criteria were identified: flow-rate limitations, "water quality volumes" (to be treated), volume reduction (through infiltration or evapotranspiration), and non-hydrologic criteria (such as loads reduction targets or maximum effluent concentrations). This study suggests that hydrologic criteria based on volume reduction (rather than treatment) might generally be preferable for on-site control of diffuse stormwater pollution. Nonetheless, determination of an appropriate management approach for a specific site is generally not straightforward and presents a variety of challenges for site designers seeking to satisfy local requirements in addressing stormwater quantity and quality issues. The adoption of efficient LID solution may therefore strongly depend on the guidance given to practitioners to account for these management criteria.

  2. Stormwater management criteria for on-site pollution control: a comparative assessment of international practices.

    PubMed

    Sage, Jérémie; Berthier, Emmanuel; Gromaire, Marie-Christine

    2015-07-01

    Over the last decade, a growing interest has been shown toward innovative stormwater management practices, breaking away from conventional "end of pipe" approaches (based on conveying water offsite to centralized detention facilities). Innovative strategies, referred to as sustainable urban drainage systems, low impact development (LID) or green infrastructures, advocating for management of runoff as close to its origin as possible, have therefore gained a lot of popularity among practitioners and public authorities. However, while the need for pollution control is generally well accepted, there is no wide agreement about management criteria to be given to developers. This article hence aims to compare these criteria through literature analysis of different state or local stormwater management manuals or guidelines, investigating both their suitability for pollution control and their influence on best management practices selection and design. Four categories of criteria were identified: flow-rate limitations, "water quality volumes" (to be treated), volume reduction (through infiltration or evapotranspiration), and non-hydrologic criteria (such as loads reduction targets or maximum effluent concentrations). This study suggests that hydrologic criteria based on volume reduction (rather than treatment) might generally be preferable for on-site control of diffuse stormwater pollution. Nonetheless, determination of an appropriate management approach for a specific site is generally not straightforward and presents a variety of challenges for site designers seeking to satisfy local requirements in addressing stormwater quantity and quality issues. The adoption of efficient LID solution may therefore strongly depend on the guidance given to practitioners to account for these management criteria. PMID:25855072

  3. Border separation for adjacent orthogonal fields

    SciTech Connect

    Werner, B.L.; Khan, F.M.; Sharma, S.C.; Lee, C.K.; Kim, T.H. )

    1991-06-01

    Field border separations for adjacent orthogonal fields can be calculated geometrically, given the validity of some important assumptions such as beam alignment and field uniformity. Thermoluminescent dosimetry (TLD) measurements were used to investigate dose uniformity across field junctions as a function of field separation and, in particular, to review the CCSG recommendation for the treatment of medulloblastoma with separate head and spine fields.

  4. 77 FR 9969 - Johnson Controls D/B/A Hoover Universal, Inc. Including On-Site Leased Workers from Kelly...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-21

    ... 16, 2010 (75 FR 34177). At the request of the State agency, the Department reviewed the certification... Employment and Training Administration Johnson Controls D/B/A Hoover Universal, Inc. Including On-Site Leased... Adjustment Assistance on June 1, 2010, applicable to workers of Johnson Controls, including on-site...

  5. Oxidation of an Adjacent Methionine Residue Inhibits Regulatory Seryl-phosphorylation of Pyruvate Dehydrogenase

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A Met residue is located adjacent to phosphorylation site 1 in the sequences of mitochondrial pyruvate dehydrogenase E1alpha subunits. When synthetic peptides including site 1 were treated with Hydrogen peroxide, the Met residue was oxidized to methionine sulfoxide (MetSO), and the peptides were no...

  6. Preoperative hair removal and surgical site infections: network meta-analysis of randomized controlled trials.

    PubMed

    Lefebvre, A; Saliou, P; Lucet, J C; Mimoz, O; Keita-Perse, O; Grandbastien, B; Bruyère, F; Boisrenoult, P; Lepelletier, D; Aho-Glélé, L S

    2015-10-01

    Preoperative hair removal has been used to prevent surgical site infections (SSIs) or to prevent hair from interfering with the incision site. We aimed to update the meta-analysis of published randomized controlled trials about hair removal for the prevention of SSIs, and conduct network meta-analyses to combine direct and indirect evidence and to compare chemical depilation with clipping. The PubMed, ScienceDirect and Cochrane databases were searched for randomized controlled trials analysing different hair removal techniques and no hair removal in similar groups. Paired and network meta-analyses were conducted. Two readers independently assessed the study limitations for each selected article according to the Grading of Recommendations Assessment, Development and Evaluation (GRADE) method. Nineteen studies met the inclusion criteria. No study compared clipping with chemical depilation. Network meta-analyses with shaving as the reference showed significantly fewer SSIs with clipping, chemical depilation, or no depilation [relative risk 0.55, 95% confidence interval 0.38-0.79; 0.60, 0.36-0.97; and 0.56, 0.34-0.96, respectively]. No significant difference was observed between the absence of depilation and chemical depilation or clipping (1.05, 0.55-2.00; 0.97, 0.51-1.82, respectively] or between chemical depilation and clipping (1.09, 0.59-2.01). This meta-analysis of 19 randomized controlled trials confirmed the absence of any benefit of depilation to prevent surgical site infection, and the higher risk of surgical site infection when shaving is used for depilation. Chemical depilation and clipping were compared for the first time. The risk of SSI seems to be similar with both methods. PMID:26320612

  7. Influence of a Brazilian sewage outfall on the toxicity and contamination of adjacent sediments

    USGS Publications Warehouse

    Abessa, D.M.S.; Carr, R.S.; Rachid, B.R.F.; Sousa, E.C.P.M.; Hortelani, M.A.; Sarkis, J.E.

    2005-01-01

    The submarine sewage outfall of Santos (SSOS) is situated in the Santos Bay (São Paulo, Brazil) and is potentially a significant source of contaminants to the adjacent marine ecosystem. The present study aimed to assess the influence of SSOS on the sediment toxicity and contamination at Santos Bay. At the disposal site, sediments tended to be finer, organically richer and exhibited higher levels of surfactants and metals, sometimes exceeding the “Threshold Effect Level” values. The SSOS influence was more evident toward the East, where the sediments exhibited higher levels of TOC, total S and metals during the summer 2000 sampling campaign. Sediment toxicity to amphipods was consistently detected in four of the five stations studied. Amphipod survival tended to correlate negatively to Hg, total N and % mud. This work provides evidence that the SSOS discharge affects the quality of sediments from Santos Bay, and that control procedures are warranted.

  8. Leaching and persistence of herbicides for kudzu (Pueraria montana) control on pine regeneration sites

    SciTech Connect

    Berisford, Yvette, C.; Bush, Parshall, B.; Taylor, John, W.

    2006-03-01

    Kudzu is an exotic vine that threatens forests in the southeastern United States. It can climb, overtop, and subsequently kill new seedlings or mature trees. Herbicides are commonly used to control kudzu; however, eradication might require retreatment for 3 to 10 yr in young stands and 7 to 10 yr for mature stands. Clopyralid, picloram, triclopyr, metsulfuron, and tebuthiuron exert various degrees of control, depending on soil type, meteorological conditions, herbicide formulation, seasonal application, characteristics of the kudzu stand, and frequency and number of herbicide. Field residue data for soil or leachate are lacking for all of these herbicides when they are used in actual forest regeneration programs in the Coastal Plain. These data are needed to assess the relative potential for the herbicides to leach into groundwater or to move off-site into sensitive ecological areas of the Coastal Plain in which sandy soils predominate and the groundwater tends to be shallow. As part of an integrated pest management program to control kudzu on forest regeneration areas at the Savannah River Site near New Ellenton, SC, five herbicides were evaluated from the standpoints of herbicide leaching, kudzu control, and plant community development. Three herbicide chemical families were represented. This included pyridinecarboxylic acid herbicides (clopyralid, picloram 1 2,4-D, and triclopyr), a sulfonylurea herbicide (metsulfuron), and a substituted urea herbicide (tebuthiuron).

  9. Prestress and Adhesion Site Dynamics Control Cell Sensitivity to Extracellular Stiffness

    PubMed Central

    Féréol, S.; Fodil, R.; Laurent, V.M.; Balland, M.; Louis, B.; Pelle, G.; Hénon, S.; Planus, E.; Isabey, D.

    2009-01-01

    This study aims at improving the understanding of mechanisms responsible for cell sensitivity to extracellular environment. We explain how substrate mechanical properties can modulate the force regulation of cell sensitive elements primarily adhesion sites. We present a theoretical and experimental comparison between two radically different approaches of the force regulation of adhesion sites that depends on their either stationary or dynamic behavior. The most classical stationary model fails to predict cell sensitivity to substrate stiffness whereas the dynamic model predicts extracellular stiffness dependence. This is due to a time dependent reaction force in response to actomyosin traction force exerted on cell sensitive elements. We purposely used two cellular models, i.e., alveolar epithelial cells and alveolar macrophages exhibiting respectively stationary and dynamic adhesion sites, and compared their sensitivity to theoretical predictions. Mechanical and structural results show that alveolar epithelial cells exhibit significant prestress supported by evident stress fibers and lacks sensitivity to substrate stiffness. On the other hand, alveolar macrophages exhibit low prestress and exhibit sensitivity to substrate stiffness. Altogether, theory and experiments consistently show that adhesion site dynamics and cytoskeleton prestress control cell sensitivity to extracellular environment with an optimal sensitivity expected in the intermediate range. PMID:19254561

  10. Tuned by metals: the TET peptidase activity is controlled by 3 metal binding sites

    PubMed Central

    Colombo, Matteo; Girard, Eric; Franzetti, Bruno

    2016-01-01

    TET aminopeptidases are dodecameric particles shared in the three life domains involved in various biological processes, from carbon source provider in archaea to eye-pressure regulation in humans. Each subunit contains a dinuclear metal site (M1 and M2) responsible for the enzyme catalytic activity. However, the role of each metal ion is still uncharacterized. Noteworthy, while mesophilic TETs are activated by Mn2+, hyperthermophilic TETs prefers Co2+. Here, by means of anomalous x-ray crystallography and enzyme kinetics measurements of the TET3 aminopeptidase from the hyperthermophilic organism Pyrococcus furiosus (PfTET3), we show that M2 hosts the catalytic activity of the enzyme, while M1 stabilizes the TET3 quaternary structure and controls the active site flexibility in a temperature dependent manner. A new third metal site (M3) was found in the substrate binding pocket, modulating the PfTET3 substrate preferences. These data show that TET activity is tuned by the molecular interplay among three metal sites. PMID:26853450

  11. Towards site-resolved imaging and control of ultracold fermions in optical lattices

    NASA Astrophysics Data System (ADS)

    Huber, Florian; Setiawan, Widagdo; Wooley-Brown, Kate; Parsons, Maxwell; Blatt, Sebastian; Greiner, Markus

    2012-06-01

    Recent successes in site-resolved imaging and control of bosonic Rb atoms trapped in optical lattices have enable many new possibilities to emulate simple condensed matter systems. Many of the open questions in condensed matter, however, stem from the fermionic nature of electrons. Extending the high degree of control available with ultracold quantum gases in optical lattices to fermionic atoms will allow us to address these questions. The light mass of fermionic 6-Li leads to system dynamics on fast timescales, making it an ideal candidate for such studies. We report progress towards a 6-Li quantum gas microscope and present improved imaging, cooling, and trapping techniques compatible with the light mass of 6-Li. A major challenge in the pursuit of single-site imaging with Lithium is cooling atoms during the imaging process. Single-site experiments with bosons benefit from the resolved hyperfine splitting in the excited state of 87-Rb, which allows the use of optical molasses. This method cannot be straightforwardly applied to 6-Li. We present our efforts to cool and image 6-Li using Raman sideband cooling.

  12. Institutional Control Policies and Implementation for the Area 5 and Area 3 Radioactive Waste Management Sites

    SciTech Connect

    Vefa Yucel, Greg Shott, Denise Wieland, et al.

    2007-11-01

    The U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office (NNSA/NSO) has implemented varying institutional control policies in performance assessment/composite analysis (PA/CA) calculations for the Area 5 and Area 3 Radioactive Waste Management Sites (RWMSs) (Shott et al., 1998; 2000; Bechtel Nevada [BN] and Neptune and Company Inc. [Neptune], 2006). The facilities are within the actively maintained boundaries of the Nevada Test Site (NTS) that are enforced by NNSA/NSO. Under current policies, access required for exposure of the member of public (MOP) or the inadvertent human intruder (IHI) is prohibited. Uncertainties affecting institutional control policies are the duration and effectiveness of the controls during the post-closure period. Implementing a uniform set of institutional control policies for the RWMSs that encompasses waste management and environmental restoration programs and is consistent with the end-state vision for the environmental management programs for the NTS (DOE, 2006) is a primary goal of the maintenance program. The NNSA/NSO Performance Management Plan (DOE, 2002) complies with DOE Policy P455.1, 'Use of Risk-Based End States' (DOE, 2003a). Expected future land uses are a driver in selecting acceptable end state conditions and clean-up goals for the NTS. NNSA/NSO Environmental Management's (EM's) land management assumptions and framework for Environmental Management activities are as follows: The NTS will remain under federal control in perpetuity as an NNSA test site, and the large buffer zone surrounding the NTS (the Nevada Test and Training Range) is assumed to remain under the control of the U.S. Air Force. There are no plans for transfer of any NTS lands to other agencies or public entities. Access will continue to be restricted to the NTS and the surrounding areas. For management purposes, NNSA/NV EM activities have been established based on the source of contamination and type of waste

  13. Imaging After GliaSite Brachytherapy: Prognostic MRI Indicators of Disease Control and Recurrence

    SciTech Connect

    Kleinberg, Lawrence; Yoon, Geoffrey; Weingart, John D.; Parisi, Michele; Olivi, Alessandro; Detorie, Nicholas A.; Chan, Timothy A.

    2009-12-01

    framework for the analysis of disease control in future prospective studies of GliaSite treatment.

  14. A multi-site controlled trial of a cognitive skills program for mentally disordered offenders

    PubMed Central

    2012-01-01

    Background The effectiveness of offending behaviour programs in forensic mental health settings is not well established. Thus this study aimed to evaluate the Reasoning and Rehabilitation Mental Health program (R&R2 MHP) among a mentally disordered offender (MDO) population. Methods A sample of 121 adult males drawn from 10 forensic mental health sites completed questionnaires at baseline and post-treatment to assess violent attitudes, locus of control, social problem-solving and anger. An informant measure of social and psychological functioning, including disruptive behaviour, was completed by unit staff at the same time. At three month follow-up patients completed again the violent attitudes and locus of control questionnaires. The data of 67 patients who participated in the group condition were compared with 54 waiting-list controls who received treatment as usual. Results 78% of group participants completed the program. In contrast to controls, significant treatment effects were found at outcome on self-reported measures of violent attitudes, rational problem-solving and anger cognitions. Improvements were endorsed by informant ratings of social and psychological functioning within the establishments. At follow-up significant treatment effects were found for both violent attitudes and locus of control. Conclusions R&R2 MHP was effective in a sample of MDOs and had a comparatively low drop-out rate. Future research should use a randomized controlled design. PMID:22607165

  15. Role of Epidural and Patient-Controlled Analgesia in Site-Specific Laparoscopic Colorectal Surgery

    PubMed Central

    Kamiński, Jan P.; Pai, Ajit; Ailabouni, Luay; Marecik, Slawomir J.; Prasad, Leela M.; Abcarian, Herand

    2014-01-01

    Background and Objectives: Limited data are available comparing epidural and patient-controlled analgesia in site-specific colorectal surgery. The aim of this study was to evaluate 2 modes of analgesia in patients undergoing laparoscopic right colectomy (RC) and low anterior resection (LAR). Methods: Prospectively collected data on 433 patients undergoing laparoscopic or laparoscopic-assisted colon surgery at a single institution were retrospectively reviewed from March 2004 to February 2009. Patients were divided into groups undergoing RC (n = 175) and LAR (n = 258). These groups were evaluated by use of analgesia: epidural analgesia, “patient-controlled analgesia” alone, and a combination of both. Demographic and perioperative outcomes were compared. Results: Epidural analgesia was associated with a faster return of bowel function, by 1 day (P < .001), in patients who underwent LAR but not in the RC group. Delayed return of bowel function was associated with increased operative time in the LAR group (P = .05), patients with diabetes who underwent RC (P = .037), and patients after RC with combined analgesia (P = .011). Mean visual analogue scale pain scores were significantly lower with epidural analgesia compared with patient-controlled analgesia in both LAR and RC groups (P < .001). Conclusion: Epidural analgesia was associated with a faster return of bowel function in the laparoscopic LAR group but not the RC group. Epidural analgesia was superior to patient-controlled analgesia in controlling postoperative pain but was inadequate in 28% of patients and needed the addition of patient-controlled analgesia. PMID:25419110

  16. Ius Chasma Tributary Valleys and Adjacent Plains

    NASA Technical Reports Server (NTRS)

    2006-01-01

    This image covers valley tributaries of Ius Chasma, as well as the plains adjacent to the valleys. Ius Chasma is one of several canyons that make up the Valles Marineris canyon system. Valles Marineris likely formed by extension associated with the growth of the large volcanoes and topographic high of Tharsis to the northwest. As the ground was pulled apart, large and deep gaps resulted in the valleys seen in the top and bottom of this HiRISE image. Ice that was once in the ground could have also melted to create additional removal of material in the formation of the valleys. HiRISE is able to see the rocks along the walls of both these valleys and also impact craters in the image. Rock layers that appear lower down in elevation appear rougher and are shedding boulders. Near the top of the walls and also seen in patches along the smooth plains are brighter layers. These brighter layers are not shedding boulders so they must represent a different kind of rock formed in a different kind of environment than those further down the walls. Because they are highest in elevation, the bright layers are youngest in age. HiRISE is able to see dozens of the bright layers, which are perhaps only a meter in thickness. Darker sand dunes and ripples cover most of the plains and fill the floors of impact craters.

    Image PSP_001351_1715 was taken by the High Resolution Imaging Science Experiment (HiRISE) camera onboard the Mars Reconnaissance Orbiter spacecraft on November 9, 2006. The complete image is centered at -8.3 degrees latitude, 275.4 degrees East longitude. The range to the target site was 254.3 km (158.9 miles). At this distance the image scale ranges from 25.4 cm/pixel (with 1 x 1 binning) to 101.8 cm/pixel (with 4 x 4 binning). The image shown here has been map-projected to 25 cm/pixel and north is up. The image was taken at a local Mars time of 3:32 PM and the scene is illuminated from the west with a solar incidence angle of 59 degrees, thus the sun was about

  17. Adjacent Segment Pathology after Lumbar Spinal Fusion.

    PubMed

    Lee, Jae Chul; Choi, Sung-Woo

    2015-10-01

    One of the major clinical issues encountered after lumbar spinal fusion is the development of adjacent segment pathology (ASP) caused by increased mechanical stress at adjacent segments, and resulting in various radiographic changes and clinical symptoms. This condition may require surgical intervention. The incidence of ASP varies with both the definition and methodology adopted in individual studies; various risk factors for this condition have been identified, although a significant controversy still exists regarding their significance. Motion-preserving devices have been developed, and some studies have shown their efficacy of preventing ASP. Surgeons should be aware of the risk factors of ASP when planning a surgery, and accordingly counsel their patients preoperatively. PMID:26435804

  18. Peri-operative glycaemic control regimens for preventing surgical site infections in adults

    PubMed Central

    Kao, Lillian S; Meeks, Derek; Moyer, Virginia A; Lally, Kevin P

    2010-01-01

    Background Surgical site infections (SSIs) are associated with significant morbidity, mortality, and resource utilization and are potentially preventable. Peri-operative hyperglycaemia has been associated with increased SSIs and previous recommendations have been to treat glucose levels above 200 mg/dL. However, recent studies have questioned the optimal glycaemic control regimen to prevent SSIs. Whether the benefits of strict or intensive glycaemic control with insulin infusion as compared to conventional management outweigh the risks remains controversial. Objectives To summarise the evidence for the impact of glycaemic control in the peri-operative period on the incidence of surgical site infections, hypoglycaemia, level of glycaemic control, all-cause and infection-related mortality, and hospital length of stay and to investigate for differences of effect between different levels of glycaemic control. Search strategy A search strategy was developed to search the following databases: Cochrane Wounds Group Specialised Register (searched 25 March 2009), The Cochrane Central Register of Controlled Trials, The Cochrane Library 2009, Issue 1; Ovid MEDLINE (1950 to March Week 2 2009); Ovid EMBASE (1980 to 2009 Week 12) and EBSCO CINAHL (1982 to March Week 3 2009). The search was not limited by language or publication status. Selection criteria Randomised controlled trials (RCTs) were eligible for inclusion if they evaluated two (or more) glycaemic control regimens in the peri-operative period (within one week pre-, intra-, and/or post-operative) and reported surgical site infections as an outcome. Data collection and analysis The standard method for conducting a systematic review in accordance with the Cochrane Wounds Group was used. Two review authors independently reviewed the results from the database searches and identified relevant studies. Two review authors extracted study data and outcomes from each study and reviewed each study for methodological quality. Any

  19. EM induction and magnetic surveys at large landfill sites using GPS for postitional control

    SciTech Connect

    Hackworth, J.B.; Kirkpatrick, T.M.; Holtzclaw, D.R.

    1996-11-01

    Electromagnetic (EM) induction and magnetic surveys were conducted at two large landfill sites, totaling 76 acres, using a global positioning system (GPS) as the positional control method. The surveys were conducted at Tinker AFB in Oklahoma, as part of a Phase II Resource Conservation and Recovery Act (RCRA) facility investigation. The objectives of the investigation were to delineate the boundaries of known landfill trenches and determine whether features seen in aerial photographs represented previously unidentified trenches. The GPS method of survey control was chosen rather than the conventional local grid approach because site conditions included extremely rugged surface topography, thick vegetation, and several swampy areas that precluded the establishment of precisely spaced, straight lines. The alternative would have required civil surveying and extensive vegetation removal. The GPS allowed local navigation and re-orientation to avoid obstacles and field hazards while continuing to collect valid, accurately located geophysical data. The use of differentially corrected GPS data for this investigation proved to be an excellent way to obtain geophysical survey control at a reduced level of effort; however, it added complexity to the surveys that had to be planned for both in the field and during data processing. Specialized processing techniques were developed to overcome minor satellite signal {open_quote}dropouts,{close_quote} and to allow for proper time-synchronization and merging of the geophysical and GPS data.

  20. Solid phase electron donors control denitrification in groundwater at agricultural sites

    NASA Astrophysics Data System (ADS)

    Green, C. T.; Liao, L.; Bekins, B. A.; Bohlke, J. K.

    2011-12-01

    Increased concentrations of nitrate in groundwater caused by agricultural use of chemical and organic fertilizers are a concern because of possible risks to environmental and human health. At many sites, these problems are mitigated by natural attenuation of nitrate as a result of microbially mediated denitrification of nitrate to nitrogen gas. Recent studies have clarified the factors affecting the rates and extents of denitrification in groundwater in agricultural areas. Intensive studies were conducted by the US Geological Survey to study agricultural chemicals in California, Nebraska, Washington, and Maryland using laboratory analyses, field measurements, and flow and transport modeling for monitoring well transects (0.5 to 2.5 km in length) and vertical profiles (0 to 50 m in depth). Groundwater analyses included major ion chemistry, dissolved gases, nitrogen and oxygen stable isotopes, and atmospheric age-tracers. Sediments were analyzed for concentrations of potential electron donors for denitrification, including reduced iron and sulfur, and organic carbon. Geochemical data and mass balance calculations indicated that solid-phase electron donors were an important factor controlling denitrification at these sites. To examine the generality of this result, a mathematical model of vertical flux of water, oxygen, and nitrate was developed and applied at these study sites along with 2 new study sites in Iowa and Mississippi and 8 additional sites from previous studies in Nebraska, Texas, Minnesota, Wisconsin, North Carolina, Maryland (2 sites), and New York. Model results confirmed the importance of solid phase electron donors. The normalized reaction rates on an electron flux basis tended to increase with depth from the shallow oxygen reduction zone to the underlying nitrate reduction zone. The pattern of higher rates at depth is consistent with a reaction rate controlled by solid phase donors that are depleted under oxidizing conditions near the surface and in

  1. Adjacent Segment Pathology after Anterior Cervical Fusion

    PubMed Central

    Chung, Jae Yoon; Park, Jong-Beom; Seo, Hyoung-Yeon

    2016-01-01

    Anterior cervical fusion has become a standard of care for numerous pathologic conditions of the cervical spine. However, subsequent development of clinically significant disc disease at levels adjacent to fused discs is a serious long-term complication of this procedure. As more patients live longer after surgery, it is foreseeable that adjacent segment pathology (ASP) will develop in increasing numbers of patients. Also, ASP has been studied more intensively with the recent popularity of motion preservation technologies like total disc arthroplasty. The true nature and scope of ASP remains poorly understood. The etiology of ASP is most likely multifactorial. Various factors including altered biomechanical stresses, surgical disruption of soft tissue and the natural history of cervical disc disease contribute to the development of ASP. General factors associated with disc degeneration including gender, age, smoking and sports may play a role in the development of ASP. Postoperative sagittal alignment and type of surgery are also considered potential causes of ASP. Therefore, a spine surgeon must be particularly careful to avoid unnecessary disruption of the musculoligamentous structures, reduced risk of direct injury to the disc during dissection and maintain a safe margin between the plate edge and adjacent vertebrae during anterior cervical fusion. PMID:27340541

  2. Adjacent Segment Pathology after Anterior Cervical Fusion.

    PubMed

    Chung, Jae Yoon; Park, Jong-Beom; Seo, Hyoung-Yeon; Kim, Sung Kyu

    2016-06-01

    Anterior cervical fusion has become a standard of care for numerous pathologic conditions of the cervical spine. However, subsequent development of clinically significant disc disease at levels adjacent to fused discs is a serious long-term complication of this procedure. As more patients live longer after surgery, it is foreseeable that adjacent segment pathology (ASP) will develop in increasing numbers of patients. Also, ASP has been studied more intensively with the recent popularity of motion preservation technologies like total disc arthroplasty. The true nature and scope of ASP remains poorly understood. The etiology of ASP is most likely multifactorial. Various factors including altered biomechanical stresses, surgical disruption of soft tissue and the natural history of cervical disc disease contribute to the development of ASP. General factors associated with disc degeneration including gender, age, smoking and sports may play a role in the development of ASP. Postoperative sagittal alignment and type of surgery are also considered potential causes of ASP. Therefore, a spine surgeon must be particularly careful to avoid unnecessary disruption of the musculoligamentous structures, reduced risk of direct injury to the disc during dissection and maintain a safe margin between the plate edge and adjacent vertebrae during anterior cervical fusion. PMID:27340541

  3. Structural analysis of site-controlled InAs/InP quantum dots

    NASA Astrophysics Data System (ADS)

    Fain, B.; Elvira, D.; Le Gratiet, L.; Largeau, L.; Beaudoin, G.; Troadec, D.; Abram, I.; Beveratos, A.; Robert-Philip, I.; Patriarche, G.; Sagnes, I.

    2011-11-01

    We present atomic-scale characterization of site-controlled InAs/InP(001) quantum dots grown by metal-organic chemical vapor deposition using nano-area selective area growth. We have developed for this purpose a process combining e-beam lithography, inductively coupled-plasma etching and focused ion beam etching to isolate a few quantum dots. The size, the shape and the composition of the quantum dots are investigated by Scanning Transmission Electron Microscopy. A comparison with the well-known single self-assembled quantum dots highlights the specificities of our growth mode compared to the Stranski-Krastanov growth mode.

  4. Using Social Networking Sites for Communicable Disease Control: Innovative Contact Tracing or Breach of Confidentiality?

    PubMed Central

    Mandeville, Kate L.; Harris, Matthew; Thomas, H. Lucy; Chow, Yimmy; Seng, Claude

    2014-01-01

    Social media applications such as Twitter, YouTube and Facebook have attained huge popularity, with more than three billion people and organizations predicted to have a social networking account by 2015. Social media offers a rapid avenue of communication with the public and has potential benefits for communicable disease control and surveillance. However, its application in everyday public health practice raises a number of important issues around confidentiality and autonomy. We report here a case from local level health protection where the friend of an individual with meningococcal septicaemia used a social networking site to notify potential contacts. PMID:24688599

  5. Role of site-selective cAMP analogs in the control and reversal of malignancy.

    PubMed

    Cho-Chung, Y S; Clair, T; Tortora, G; Yokozaki, H

    1991-01-01

    Two isoforms of cAMP receptor protein, RI and RII, the regulatory subunits of cAMP-dependent protein kinase, transduce opposite signals, the RI being stimulatory and the RII being inhibitory of cell proliferation. In normal cells RI and RII exist at a specific physiological ratio whereas in cancer cells such physiological balance of these receptor proteins is disrupted. Reversal and suppression of malignancy can be achieved when the physiologic ratio of these intracellular signal transducers of cAMP is restored as shown by the use of site-selective cAMP analogs, antisense oligodeoxynucleotides or gene transfer, suggesting new approaches to cancer control. PMID:1653961

  6. Using Social Networking Sites for Communicable Disease Control: Innovative Contact Tracing or Breach of Confidentiality?

    PubMed

    Mandeville, Kate L; Harris, Matthew; Thomas, H Lucy; Chow, Yimmy; Seng, Claude

    2014-04-01

    Social media applications such as Twitter, YouTube and Facebook have attained huge popularity, with more than three billion people and organizations predicted to have a social networking account by 2015. Social media offers a rapid avenue of communication with the public and has potential benefits for communicable disease control and surveillance. However, its application in everyday public health practice raises a number of important issues around confidentiality and autonomy. We report here a case from local level health protection where the friend of an individual with meningococcal septicaemia used a social networking site to notify potential contacts. PMID:24688599

  7. Charging dynamics of a floating gate transistor with site-controlled quantum dots

    SciTech Connect

    Maier, P. Hartmann, F.; Emmerling, M.; Schneider, C.; Höfling, S.; Kamp, M.; Worschech, L.

    2014-08-04

    A quantum dot memory based on a GaAs/AlGaAs quantum wire with site-controlled InAs quantum dots was realized by means of molecular beam epitaxy and etching techniques. By sampling of different gate voltage sweeps for the determination of charging and discharging thresholds, it was found that discharging takes place at short time scales of μs, whereas several seconds of waiting times within a distinct negative gate voltage range were needed to charge the quantum dots. Such quantum dot structures have thus the potential to implement logic functions comprising charge and time dependent ingredients such as counting of signals or learning rules.

  8. Controlled Soil Warming Powered by Alternative Energy for Remote Field Sites

    PubMed Central

    Johnstone, Jill F.; Henkelman, Jonathan; Allen, Kirsten; Helgason, Warren; Bedard-Haughn, Angela

    2013-01-01

    Experiments using controlled manipulation of climate variables in the field are critical for developing and testing mechanistic models of ecosystem responses to climate change. Despite rapid changes in climate observed in many high latitude and high altitude environments, controlled manipulations in these remote regions have largely been limited to passive experimental methods with variable effects on environmental factors. In this study, we tested a method of controlled soil warming suitable for remote field locations that can be powered using alternative energy sources. The design was tested in high latitude, alpine tundra of southern Yukon Territory, Canada, in 2010 and 2011. Electrical warming probes were inserted vertically in the near-surface soil and powered with photovoltaics attached to a monitoring and control system. The warming manipulation achieved a stable target warming of 1.3 to 2°C in 1 m2 plots while minimizing disturbance to soil and vegetation. Active control of power output in the warming plots allowed the treatment to closely match spatial and temporal variations in soil temperature while optimizing system performance during periods of low power supply. Active soil heating with vertical electric probes powered by alternative energy is a viable option for remote sites and presents a low-disturbance option for soil warming experiments. This active heating design provides a valuable tool for examining the impacts of soil warming on ecosystem processes. PMID:24386125

  9. A Bistable Switch and Anatomical Site Control Vibrio cholerae Virulence Gene Expression in the Intestine

    PubMed Central

    Nielsen, Alex T.; Dolganov, Nadia A.; Rasmussen, Thomas; Otto, Glen; Miller, Michael C.; Felt, Stephen A.; Torreilles, Stéphanie; Schoolnik, Gary K.

    2010-01-01

    A fundamental, but unanswered question in host-pathogen interactions is the timing, localization and population distribution of virulence gene expression during infection. Here, microarray and in situ single cell expression methods were used to study Vibrio cholerae growth and virulence gene expression during infection of the rabbit ligated ileal loop model of cholera. Genes encoding the toxin-coregulated pilus (TCP) and cholera toxin (CT) were powerfully expressed early in the infectious process in bacteria adjacent to epithelial surfaces. Increased growth was found to co-localize with virulence gene expression. Significant heterogeneity in the expression of tcpA, the repeating subunit of TCP, was observed late in the infectious process. The expression of tcpA, studied in single cells in a homogeneous medium, demonstrated unimodal induction of tcpA after addition of bicarbonate, a chemical inducer of virulence gene expression. Striking bifurcation of the population occurred during entry into stationary phase: one subpopulation continued to express tcpA, whereas the expression declined in the other subpopulation. ctxA, encoding the A subunit of CT, and toxT, encoding the proximal master regulator of virulence gene expression also exhibited the bifurcation phenotype. The bifurcation phenotype was found to be reversible, epigenetic and to persist after removal of bicarbonate, features consistent with bistable switches. The bistable switch requires the positive-feedback circuit controlling ToxT expression and formation of the CRP-cAMP complex during entry into stationary phase. Key features of this bistable switch also were demonstrated in vivo, where striking heterogeneity in tcpA expression was observed in luminal fluid in later stages of the infection. When this fluid was diluted into artificial seawater, bacterial aggregates continued to express tcpA for prolonged periods of time. The bistable control of virulence gene expression points to a mechanism that could

  10. CONTAMINATION CONTROL DURING IN SITU JET GROUTING FOR APPLICATION IN A BURIED TRANSURANIC WASTE SITE

    SciTech Connect

    Loomis, Guy G.; Jessmore, Jim J.

    2003-02-27

    Engineers at the Idaho National Engineering and Environmental Laboratory (INEEL) have developed means of contamination control associated with jet-grouting buried radioactive mixed waste sites. Finely divided plutonium/americium oxide particulate can escape as the drill stem of the jet-grouting apparatus exits a waste deposit in preparation for insertion in another injection hole. In studying various options for controlling this potential contamination, engineers found that an elaborate glovebox/drill string shroud system prevents contaminants from spreading. Researchers jet-grouted a pit with nonradioactive tracers to simulate the movement of plutonium fines during an actual application. Data from the testing indicate that the grout immobilizes the tracer material by locking it up in particles large enough to resist aerosolization.

  11. Contamination Control During In Situ Jet Grouting for Application in a Buried Transuranic Waste Site

    SciTech Connect

    Loomis, Guy George; Jessmore, James Joseph

    2003-02-01

    Engineers at the Idaho National Engineering and Environmental Laboratory (INEEL) have developed means of contamination control associated with jet-grouting buried radioactive mixed waste sites. Finely divided plutonium/americium oxide particulate can escape as the drill stem of the jet-grouting apparatus exits a waste deposit in preparation for insertion in another injection hole. In studying various options for controlling this potential contamination, engineers found that an elaborate glovebox/drill string shroud system prevents contaminants from spreading. Researchers jet-grouted a pit with nonradioactive tracers to simulate the movement of plutonium fines during an actual application. Data from the testing indicate that the grout immobilizes the tracer material by locking it up in particles large enough to resist aerosolization.

  12. Memristive operation mode of a site-controlled quantum dot floating gate transistor

    SciTech Connect

    Maier, P. Hartmann, F.; Mauder, T.; Emmerling, M.; Schneider, C.; Kamp, M.; Worschech, L.; Höfling, S.

    2015-05-18

    We have realized a floating gate transistor based on a GaAs/AlGaAs heterostructure with site-controlled InAs quantum dots. By short-circuiting the source contact with the lateral gates and performing closed voltage sweep cycles, we observe a memristive operation mode with pinched hysteresis loops and two clearly distinguishable conductive states. The conductance depends on the quantum dot charge which can be altered in a controllable manner by the voltage value and time interval spent in the charging region. The quantum dot memristor has the potential to realize artificial synapses in a state-of-the-art opto-electronic semiconductor platform by charge localization and Coulomb coupling.

  13. The PLATO Dome A site-testing observatory: Power generation and control systems

    NASA Astrophysics Data System (ADS)

    Lawrence, J. S.; Ashley, M. C. B.; Hengst, S.; Luong-van, D. M.; Storey, J. W. V.; Yang, H.; Zhou, X.; Zhu, Z.

    2009-06-01

    The atmospheric conditions above Dome A, a currently unmanned location at the highest point on the Antarctic plateau, are uniquely suited to astronomy. For certain types of astronomy Dome A is likely to be the best location on the planet, and this has motivated the development of the Plateau Observatory (PLATO). PLATO was deployed to Dome A in early 2008. It houses a suite of purpose-built site-testing instruments designed to quantify the benefits of Dome A site for astronomy, and science instruments designed to take advantage of the observing conditions. The PLATO power generation and control system is designed to provide continuous power and heat, and a high-reliability command and communications platform for these instruments. PLATO has run and collected data throughout the winter 2008 season completely unattended. Here we present a detailed description of the power generation, power control, thermal management, instrument interface, and communications systems for PLATO, and an overview of the system performance for 2008.

  14. Control of substrate access to the active site in methane monooxygenase.

    PubMed

    Lee, Seung Jae; McCormick, Michael S; Lippard, Stephen J; Cho, Uhn-Soo

    2013-02-21

    Methanotrophs consume methane as their major carbon source and have an essential role in the global carbon cycle by limiting escape of this greenhouse gas to the atmosphere. These bacteria oxidize methane to methanol by soluble and particulate methane monooxygenases (MMOs). Soluble MMO contains three protein components, a 251-kilodalton hydroxylase (MMOH), a 38.6-kilodalton reductase (MMOR), and a 15.9-kilodalton regulatory protein (MMOB), required to couple electron consumption with substrate hydroxylation at the catalytic diiron centre of MMOH. Until now, the role of MMOB has remained ambiguous owing to a lack of atomic-level information about the MMOH-MMOB (hereafter termed H-B) complex. Here we remedy this deficiency by providing a crystal structure of H-B, which reveals the manner by which MMOB controls the conformation of residues in MMOH crucial for substrate access to the active site. MMOB docks at the α(2)β(2) interface of α(2)β(2)γ(2) MMOH, and triggers simultaneous conformational changes in the α-subunit that modulate oxygen and methane access as well as proton delivery to the diiron centre. Without such careful control by MMOB of these substrate routes to the diiron active site, the enzyme operates as an NADH oxidase rather than a monooxygenase. Biological catalysis involving small substrates is often accomplished in nature by large proteins and protein complexes. The structure presented in this work provides an elegant example of this principle. PMID:23395959

  15. Interaction of Cracks Between Two Adjacent Indents in Glass

    NASA Technical Reports Server (NTRS)

    Choi, S. R.; Salem, J. A.

    1993-01-01

    Experimental observations of the interaction behavior of cracks between two adjacent indents were made using an indentation technique in soda-lime glass. It was specifically demonstrated how one indent crack initiates and propagates in the vicinity of another indent crack. Several types of crack interactions were examined by changing the orientation and distance of one indent relative to the other. It was found that the residual stress field produced by elastic/plastic indentation has a significant influence on controlling the mode of crack interaction. The interaction of an indent crack with a free surface was also investigated for glass and ceramic specimens.

  16. The changing role of Material Control and Accountability at Savannah River Site

    SciTech Connect

    Rodriguez, M.P.

    1994-07-01

    As Westinghouse Savannah River Company has been faced with the challenge of better meeting DOE needs with reduced budgets and manpower, the Materials Control and Accountability (MC&A) organization has taken a hard look at its roles and responsibilities. A MC&A program is composed of many functions that can not only meet safeguards needs, but can be used by several organizations across the site to meet their needs as well. These functions include nuclear material measurements, tracking, accounting, and inventory control. The infrastructure in place to provide these functions for accountable nuclear materials requires only a few adjustments to expand to other areas of nuclear materials accounting and control. By integrating several organizations` requirements, the MC&A section can allow line organizations to reduce their costs and rely on the section to better service their needs. On the reverse side, MC&A has completed several cost reduction measures that will allow it to expand its role with no increased costs. The roles and responsibilities of the nuclear material control and accountability program should be expanded. The program`s existing information infrastructure, and knowledge and experience in nuclear material measurements and safeguards can be built upon to meet the needs of new areas such as waste management and decommissioning and decontamination while continuing to support the existing processing. and storage efforts of current facilities.

  17. Reconstructing genome mixtures from partial adjacencies.

    PubMed

    Mahmoody, Ahmad; Kahn, Crystal L; Raphael, Benjamin J

    2012-01-01

    Many cancer genome sequencing efforts are underway with the goal of identifying the somatic mutations that drive cancer progression. A major difficulty in these studies is that tumors are typically heterogeneous, with individual cells in a tumor having different complements of somatic mutations. However, nearly all DNA sequencing technologies sequence DNA from multiple cells, thus resulting in measurement of mutations from a mixture of genomes. Genome rearrangements are a major class of somatic mutations in many tumors, and the novel adjacencies (i.e. breakpoints) resulting from these rearrangements are readily detected from DNA sequencing reads. However, the assignment of each rearrangement, or adjacency, to an individual cancer genome in the mixture is not known. Moreover, the quantity of DNA sequence reads may be insufficient to measure all rearrangements in all genomes in the tumor. Motivated by this application, we formulate the k-minimum completion problem (k-MCP). In this problem, we aim to reconstruct k genomes derived from a single reference genome, given partial information about the adjacencies present in the mixture of these genomes. We show that the 1-MCP is solvable in linear time in the cases where: (i) the measured, incomplete genome has a single circular or linear chromosome; (ii) there are no restrictions on the chromosomal content of the measured, incomplete genome. We also show that the k-MCP problem, for k ≥ 3 in general, and the 2-MCP problem with the double-cut-and-join (DCJ) distance are NP-complete, when there are no restriction on the chromosomal structure of the measured, incomplete genome. These results lay the foundation for future algorithmic studies of the k-MCP and the application of these algorithms to real cancer sequencing data. PMID:23282028

  18. Hydrologic Characteristics of Low-Impact Stormwater Control Measures at Two Sites in Northeastern Ohio, 2008-2013

    EPA Science Inventory

    This report updates and examines hydrologic data gathered to characterize the performance of two stormwater control measures (SCM) sites in the Chagrin River watershed, Ohio. At the Sterncrest Drive site, roadside bioswales and rain gardens were used to alleviate drainage problem...

  19. Residual herbicide study on selected Hanford Site roadsides

    SciTech Connect

    Smith, J.L.; Kemp, C.J.; Sackschewsky, M.R.

    1993-08-01

    Westinghouse Hanford Company routinely treats roadsides with herbicides to control undesirable plant growth. An experiment was conducted to test perennial grass germination in soils adjacent to roadways of the Hanford Site. The primary variable was the distance from the roadside. A simple germination test was executed in a controlled-environment chamber to determine the residual effects of these applications. As expected, the greatest herbicide activity was found directly adjacent to the roadway, approximately 0 to 20 ft (0 to 6.3 m) from the roadway.

  20. An external sodium ion binding site controls allosteric gating in TRPV1 channels.

    PubMed

    Jara-Oseguera, Andres; Bae, Chanhyung; Swartz, Kenton J

    2016-01-01

    TRPV1 channels in sensory neurons are integrators of painful stimuli and heat, yet how they integrate diverse stimuli and sense temperature remains elusive. Here, we show that external sodium ions stabilize the TRPV1 channel in a closed state, such that removing the external ion leads to channel activation. In studying the underlying mechanism, we find that the temperature sensors in TRPV1 activate in two steps to favor opening, and that the binding of sodium to an extracellular site exerts allosteric control over temperature-sensor activation and opening of the pore. The binding of a tarantula toxin to the external pore also exerts control over temperature-sensor activation, whereas binding of vanilloids influences temperature-sensitivity by largely affecting the open/closed equilibrium. Our results reveal a fundamental role of the external pore in the allosteric control of TRPV1 channel gating and provide essential constraints for understanding how these channels can be tuned by diverse stimuli. PMID:26882503

  1. An external sodium ion binding site controls allosteric gating in TRPV1 channels

    PubMed Central

    Jara-Oseguera, Andres; Bae, Chanhyung; Swartz, Kenton J

    2016-01-01

    TRPV1 channels in sensory neurons are integrators of painful stimuli and heat, yet how they integrate diverse stimuli and sense temperature remains elusive. Here, we show that external sodium ions stabilize the TRPV1 channel in a closed state, such that removing the external ion leads to channel activation. In studying the underlying mechanism, we find that the temperature sensors in TRPV1 activate in two steps to favor opening, and that the binding of sodium to an extracellular site exerts allosteric control over temperature-sensor activation and opening of the pore. The binding of a tarantula toxin to the external pore also exerts control over temperature-sensor activation, whereas binding of vanilloids influences temperature-sensitivity by largely affecting the open/closed equilibrium. Our results reveal a fundamental role of the external pore in the allosteric control of TRPV1 channel gating and provide essential constraints for understanding how these channels can be tuned by diverse stimuli. DOI: http://dx.doi.org/10.7554/eLife.13356.001 PMID:26882503

  2. Use of a marker organism in poultry processing to identify sites of cross-contamination and evaluate possible control measures.

    PubMed

    Mead, G C; Hudson, W R; Hinton, M H

    1994-07-01

    1. Nine different sites at a poultry processing plant were selected in the course of a hazard analysis to investigate the degree of microbial cross-contamination that could occur during processing and the effectiveness of possible control measures. 2. At each site, carcases, equipment or working surfaces were inoculated with a non-pathogenic strain of nalidixic acid-resistant Escherichia coli K12; transmission of the organism among carcases being processed was followed qualitatively and, where appropriate, quantitatively. 3. The degree of cross-contamination and the extent to which it could be controlled by the proposed measures varied from one site to another. PMID:7953779

  3. Comparison of mosquito control programs in seven urban sites in Africa, the Middle East, and the Americas.

    PubMed

    Impoinvil, Daniel E; Ahmad, Sajjad; Troyo, Adriana; Keating, Joseph; Githeko, Andrew K; Mbogo, Charles M; Kibe, Lydiah; Githure, John I; Gad, Adel M; Hassan, Ali N; Orshan, Laor; Warburg, Alon; Calderón-Arguedas, Olger; Sánchez-Loría, Victoria M; Velit-Suarez, Rosanna; Chadee, Dave D; Novak, Robert J; Beier, John C

    2007-10-01

    Mosquito control programs at seven urban sites in Kenya, Egypt, Israel, Costa Rica, and Trinidad are described and compared. Site-specific urban and disease characteristics, organizational diagrams, and strengths, weaknesses, obstacles and threats (SWOT) analysis tools are used to provide a descriptive assessment of each mosquito control program, and provide a comparison of the factors affecting mosquito abatement. The information for SWOT analysis is collected from surveys, focus-group discussions, and personal communication. SWOT analysis identified various issues affecting the efficiency and sustainability of mosquito control operations. The main outcome of our work was the description and comparison of mosquito control operations within the context of each study site's biological, social, political, management, and economic conditions. The issues identified in this study ranged from lack of inter-sector collaboration to operational issues of mosquito control efforts. A lack of sustainable funding for mosquito control was a common problem for most sites. Many unique problems were also identified, which included lack of mosquito surveillance, lack of law enforcement, and negative consequences of human behavior. Identifying common virtues and shortcomings of mosquito control operations is useful in identifying "best practices" for mosquito control operations, thus leading to better control of mosquito biting and mosquito-borne disease transmission. PMID:17316882

  4. Oak Ridge National Laboratory Radiation Control Program - Partners in Site Restoration

    SciTech Connect

    Jones, S. L.; Stafford, M. W.

    2002-02-26

    In 1998, the U.S. Department of Energy (DOE) awarded the Management and Integration (M&I) contract for all five of the Oak Ridge Operations (ORO) facilities to Bechtel Jacobs Company LLC (BJC). At Oak Ridge National Laboratory (ORNL), a world renowned national laboratory and research and development facility, the BJC mission involves executing the DOE Environmental Management (EM) program. In addition to BJC's M&I contract, UT-Battelle, LLC, a not-for-profit company, is the Management and Operating (M&O) contractor for DOE on the ORNL site. As part of ORNL's EM program, legacy inactive facilities (i.e., reactors, nuclear material research facilities, burial grounds, and underground storage tanks) are transferred to BJC and are designated as remediation, decontamination and decommissioning (D&D), or long-term surveillance and maintenance (S&M) facilities. Facilities operated by both UT-Battelle and BJC are interspersed throughout the site and are usually in close proximity. Both UT-Battelle and BJC have DOE-approved Radiation Protection Programs established in accordance with 10 CFR 835. The BJC Radiological Control (RADCON) Program adapts to the M&I framework and is comprised of a combination of subcontracted program responsibilities with BJC oversight. This paper focuses on the successes and challenges of executing the BJC RADCON Program for BJC's ORNL Project through a joint M&I contractor relationship, while maintaining a positive working relationship and partnership with UT-Battelle's Radiation Protection organization.

  5. Green Tea Polyphenols Control Dysregulated Glutamate Dehydrogenase in Transgenic Mice by Hijacking the ADP Activation Site

    SciTech Connect

    Li, Changhong; Li, Ming; Chen, Pan; Narayan, Srinivas; Matschinsky, Franz M.; Bennett, Michael J.; Stanley, Charles A.; Smith, Thomas J.

    2012-05-09

    Glutamate dehydrogenase (GDH) catalyzes the oxidative deamination of L-glutamate and, in animals, is extensively regulated by a number of metabolites. Gain of function mutations in GDH that abrogate GTP inhibition cause the hyperinsulinism/hyperammonemia syndrome (HHS), resulting in increased pancreatic {beta}-cell responsiveness to leucine and susceptibility to hypoglycemia following high protein meals. We have previously shown that two of the polyphenols from green tea (epigallocatechin gallate (EGCG) and epicatechin gallate (ECG)) inhibit GDH in vitro and that EGCG blocks GDH-mediated insulin secretion in wild type rat islets. Using structural and site-directed mutagenesis studies, we demonstrate that ECG binds to the same site as the allosteric regulator, ADP. Perifusion assays using pancreatic islets from transgenic mice expressing a human HHS form of GDH demonstrate that the hyperresponse to glutamine caused by dysregulated GDH is blocked by the addition of EGCG. As observed in HHS patients, these transgenic mice are hypersensitive to amino acid feeding, and this is abrogated by oral administration of EGCG prior to challenge. Finally, the low basal blood glucose level in the HHS mouse model is improved upon chronic administration of EGCG. These results suggest that this common natural product or some derivative thereof may prove useful in controlling this genetic disorder. Of broader clinical implication is that other groups have shown that restriction of glutamine catabolism via these GDH inhibitors can be useful in treating various tumors. This HHS transgenic mouse model offers a highly useful means to test these agents in vivo.

  6. Southern ocean controls on the extent of denitrification in the southeast Pacific (ODP Site 1234)

    NASA Astrophysics Data System (ADS)

    Robinson, R. S.; Mix, A.; Martinez, P.

    2005-12-01

    A mechanistic understanding of the observed temporal changes in oceanic denitrification, the bacterial reduction of nitrate under suboxic conditions, has been sought due to the potential importance of N inventory changes and the production of N2O on the climate system. High-resolution oxygen isotope and bulk sediment δ15N records from ODP Site 1234 on the Chile Margin are presented as a record of denitrification changes within the Peru-Chile Upwelling system over the last 65ky. The character of the Site 1234 δ15N record is quite similar to that of its northern hemisphere counterparts with the exception of timing. Denitrification changes in the southeast Pacific show coherent variation with Antarctic climate, as indicated by the Byrd ice core δ18O record, rather than with northern hemisphere climate change. The high latitude polar oceans play a fundamental role in setting the physical and biological controls on subsurface oxygen supply and demand. The southern hemisphere character of the Chile margin record suggests that episodes of reduced denitrification in the SE Pacific likely represent times when more oxygen was supplied as the result of changes in the chemical composition of Subantarctic Mode Water (SAMW), which forms in the Subantarctic zone of the Southern Ocean and ventilates the low latitude thermocline. An increase in oxygen can be achieved through (1) lower temperatures/ higher ventilation rates and/or (2) reduced oxygen demand in the low latitude subsurface due to reduction in the preformed nutrient content of SAMW.

  7. Magnetically Controllable Polymer Nanotubes from a Cyclized Crosslinker for Site-Specific Delivery of Doxorubicin.

    PubMed

    Newland, Ben; Leupelt, Daniel; Zheng, Yu; Thomas, Laurent S V; Werner, Carsten; Steinhart, Martin; Wang, Wenxin

    2015-01-01

    Externally controlled site specific drug delivery could potentially provide a means of reducing drug related side effects whilst maintaining, or perhaps increasing therapeutic efficiency. The aim of this work was to develop a nanoscale drug carrier, which could be loaded with an anti-cancer drug and be directed by an external magnetic field. Using a single, commercially available monomer and a simple one-pot reaction process, a polymer was synthesized and crosslinked within the pores of an anodized aluminum oxide template. These polymer nanotubes (PNT) could be functionalized with iron oxide nanoparticles for magnetic manipulation, without affecting the large internal pore, or inherent low toxicity. Using an external magnetic field the nanotubes could be regionally concentrated, leaving areas devoid of nanotubes. Lastly, doxorubicin could be loaded to the PNTs, causing increased toxicity towards neuroblastoma cells, rendering a platform technology now ready for adaptation with different nanoparticles, degradable pre-polymers, and various therapeutics. PMID:26619814

  8. Field test of infrared thermography applied to biogas controlling in landfill sites

    NASA Astrophysics Data System (ADS)

    Madruga, Francisco J.; Muñoz, Jaime M.; González, Daniel A.; Tejero, Juan I.; Cobo, Adolfo; Gil, José L.; Conde, Olga M.; López-Higuera, Jose M.

    2007-04-01

    The gases accumulated inside the landfill as result of the fermentation of Municipal Solid Waste (MSW) known as biogas, are taking into consideration all possible uses as direct transformation into electricity. The system for collecting, regulating and controlling the biogas must include all the necessary safety features where the biogas leakage presents a high impact. Infrared thermography can be use to detect gas leakages due to the differences in temperature between the gas and the immediate surroundings. This method is able to monitor a wide area of landfill sites, quickly. This technology will not be effective if the differences in temperature are not better than five degrees. This paper describes a field test conducted to study the limitations of the infrared thermography caused by weather conditions and the moment of day or/and season when the thermal images was captured. Pipelines, borders, cells, covers, slopes and leakage (hot spots) are studied and optimum conditions are defined.

  9. Magnetically Controllable Polymer Nanotubes from a Cyclized Crosslinker for Site-Specific Delivery of Doxorubicin

    NASA Astrophysics Data System (ADS)

    Newland, Ben; Leupelt, Daniel; Zheng, Yu; Thomas, Laurent S. V.; Werner, Carsten; Steinhart, Martin; Wang, Wenxin

    2015-12-01

    Externally controlled site specific drug delivery could potentially provide a means of reducing drug related side effects whilst maintaining, or perhaps increasing therapeutic efficiency. The aim of this work was to develop a nanoscale drug carrier, which could be loaded with an anti-cancer drug and be directed by an external magnetic field. Using a single, commercially available monomer and a simple one-pot reaction process, a polymer was synthesized and crosslinked within the pores of an anodized aluminum oxide template. These polymer nanotubes (PNT) could be functionalized with iron oxide nanoparticles for magnetic manipulation, without affecting the large internal pore, or inherent low toxicity. Using an external magnetic field the nanotubes could be regionally concentrated, leaving areas devoid of nanotubes. Lastly, doxorubicin could be loaded to the PNTs, causing increased toxicity towards neuroblastoma cells, rendering a platform technology now ready for adaptation with different nanoparticles, degradable pre-polymers, and various therapeutics.

  10. Linearly polarized single photon antibunching from a site-controlled InGaN quantum dot

    SciTech Connect

    Jemsson, Tomas; Machhadani, Houssaine; Karlsson, K. Fredrik; Hsu, Chih-Wei; Holtz, Per-Olof

    2014-08-25

    We report on the observation of linearly polarized single photon antibunching in the excitonic emission from a site-controlled InGaN quantum dot. The measured second order coherence function exhibits a significant dip at zero time difference, corresponding to g{sub m}{sup 2}(0)=0.90 under continuous laser excitation. This relatively high value of g{sub m}{sup 2}(0) is well understood by a model as the combination of short exciton life time (320 ps), limited experimental timing resolution and the presence of an uncorrelated broadband background emission from the sample. Our result provides the first rigorous evidence of InGaN quantum dot formation on hexagonal GaN pyramids, and it highlights a great potential in these dots as fast polarized single photon emitters if the background emission can be eliminated.

  11. Conditions for entangled photon emission from (111)B site-controlled pyramidal quantum dots

    SciTech Connect

    Juska, G. Murray, E.; Dimastrodonato, V.; Chung, T. H.; Moroni, S. T.; Gocalinska, A.; Pelucchi, E.

    2015-04-07

    A study of highly symmetric site-controlled pyramidal In{sub 0.25}Ga{sub 0.75}As quantum dots (QDs) is presented. It is discussed that polarization-entangled photons can be also obtained from pyramidal QDs of different designs from the one already reported in Juska et al. [Nat. Photonics 7, 527 (2013)]. Moreover, some of the limitations for a higher density of entangled photon emitters are addressed. Among these issues are (1) a remaining small fine-structure splitting and (2) an effective QD charging under non-resonant excitation conditions, which strongly reduce the number of useful biexciton-exciton recombination events. A possible solution of the charging problem is investigated exploiting a dual-wavelength excitation technique, which allows a gradual QD charge tuning from strongly negative to positive and, eventually, efficient detection of entangled photons from QDs, which would be otherwise ineffective under a single-wavelength (non-resonant) excitation.

  12. The Role of Groove Periodicity in the Formation of Site-Controlled Quantum Dot Chains.

    PubMed

    Schramm, Andreas; Hakkarainen, Teemu V; Tommila, Juha; Guina, Mircea

    2015-12-01

    Structural and optical properties of InAs quantum dot (QD) chains formed in etched GaAs grooves having different periods from 200 to 2000 nm in [010] orientation are reported. The site-controlled QDs were fabricated by molecular beam epitaxy on soft UV-nanoimprint lithography-patterned GaAs(001) surfaces. Increasing the groove periods decreases the overall QD density but increases the QD size and the linear density along the groove direction. The effect of the increased QD size with larger periods is reflected in ensemble photoluminescence measurements as redshift of the QD emission. Furthermore, we demonstrate the photoluminescence emission from single QD chains. PMID:26058509

  13. Magnetically Controllable Polymer Nanotubes from a Cyclized Crosslinker for Site-Specific Delivery of Doxorubicin

    PubMed Central

    Newland, Ben; Leupelt, Daniel; Zheng, Yu; Thomas, Laurent S. V.; Werner, Carsten; Steinhart, Martin; Wang, Wenxin

    2015-01-01

    Externally controlled site specific drug delivery could potentially provide a means of reducing drug related side effects whilst maintaining, or perhaps increasing therapeutic efficiency. The aim of this work was to develop a nanoscale drug carrier, which could be loaded with an anti-cancer drug and be directed by an external magnetic field. Using a single, commercially available monomer and a simple one-pot reaction process, a polymer was synthesized and crosslinked within the pores of an anodized aluminum oxide template. These polymer nanotubes (PNT) could be functionalized with iron oxide nanoparticles for magnetic manipulation, without affecting the large internal pore, or inherent low toxicity. Using an external magnetic field the nanotubes could be regionally concentrated, leaving areas devoid of nanotubes. Lastly, doxorubicin could be loaded to the PNTs, causing increased toxicity towards neuroblastoma cells, rendering a platform technology now ready for adaptation with different nanoparticles, degradable pre-polymers, and various therapeutics. PMID:26619814

  14. Association of Chromosome Loss with Centromere-Adjacent Mitotic Recombination in a Yeast Disomic Haploid

    PubMed Central

    Campbell, D. A.; Fogel, S.

    1977-01-01

    Experiments designed to characterize the association between disomic chromosome loss and centromere-adjacent mitotic recombination were performed. Mitotic gene convertants were selected at two heteroallelic sites on the left arm of disomic chromosome III and tested for coincident chromosome loss. The principal results are: (1) Disomic chromosome loss is markedly enhanced (nearly 40-fold) over basal levels among mitotic gene convertants selected to arise close to the centromere; no such enhancement is observed among convertants selected to arise relatively far from the centromere. (2) Chromosome loss is primarily associated with proximal allele conversion at the centromere-adjacent site, and many of these convertants are reciprocally recombined in the adjacent proximal interval. (3) Partial aneuploid exceptions provisionally identified as carrying left arm telocentrics have been found. A testable model is proposed suggesting that centromere involvement in genetic recombination may precipitate segregational disfunction leading to mitotic chromosome loss. PMID:324869

  15. Geological and environmental controls on the change of eruptive style (phreatomagmatic to Strombolian-effusive) of Late Pleistocene El Caracol tuff cone and its comparison with adjacent volcanoes around the Zacapu basin (Michoacán, México)

    NASA Astrophysics Data System (ADS)

    Kshirsagar, Pooja; Siebe, Claus; Guilbaud, Marie Noëlle; Salinas, Sergio

    2016-05-01

    groundwater flow gradient) did not allow for sustained water/magma interactions at ideal conditions (ratio of 0.2). Hence, the system soon dried out due to insufficient groundwater flow toward the eruption site and the eruption turned purely magmatic. This study shows that despite of its proximity to a large water reservoir (Zacapu lake basin) and similarities with Alberca de Guadalupe maar in regard to age, climate, magma composition, and tectonic environment, several other specific parameters play a crucial role in shaping a monogenetic phreatomagmatic volcano. The case of El Caracol (phreatomagmatic style followed by magmatic during the course of a monogenetic eruption) has been reported from other volcanic fields around the globe where it might have similar causes. Furthermore, the general scarcity of this type of volcanoes in the Michoacán-Guanajuato Volcanic Field implies that the conditions required for their formation are rarely met in this region.

  16. Community Involvement as an Effective Institutional Control at the Weldon Spring Site, a U.S. Department of Energy Office of Legacy Management Site

    SciTech Connect

    Deyo, Y.E.; Pauling, T.

    2006-07-01

    The U.S. Department of Energy (DOE) Weldon Spring Site Remedial Action Project (WSSRAP) was conducted for the purpose of remediating a portion of a former trinitrotoluene and dinitrotoluene production plant that was operational from 1941 to 1945 and a former uranium refinery that was operational from 1957 to 1966. Surface remediation activities concluded in 2001 with the completion of a 45-acre (.18 square kilometer) on-site engineered disposal facility. Long-term surveillance and maintenance activities at the site were officially transferred to the DOE Office of Legacy Management in 2003. The Weldon Spring Site is located within the St. Louis, Missouri, metropolitan area (population 3 million). DOE's close relationship with surrounding land owners created a need for innovative solutions to long-term surveillance and maintenance issues at the site. Through a Secretarial proclamation, a plan was established for development of a comprehensive public involvement and education program. This program would act as an institutional control to communicate the historical legacy of the site and would make information available about contamination present at the site to guide people in making decisions about appropriate site activities. In August 2002, the Weldon Spring Site Interpretive Center opened to the public with exhibits about the history of the area, the remediation work that was completed, and a site information repository that is available to visitors. In addition, the Hamburg Trail for hiking and biking was constructed as a joint DOE/MDC effort. The 8-mile trail travels through both DOE and MDC property; a series of historical markers posted along its length to communicate the history of the area and the remediation work that was done as part of WSSRAP activities. A ramp and viewing platform with informational plaques were constructed on the disposal cell to provide an additional mechanism for public education. With a basic marketing program, site visitor-ship has

  17. Corrosion Control during Closure Activities at the Savannah River Site - 13514

    SciTech Connect

    Wiersma, Bruce J.; Subramanian, Karthik H.; Martin, Keisha B.

    2013-07-01

    Liquid radioactive wastes from the Savannah River Site (SRS) separation process are stored in large underground carbon steel tanks. Until the waste is removed from storage, transferred, and processed, the materials and structures of the tanks must maintain a confinement function by providing a barrier to the environment and by maintaining acceptable structural stability during normal service and design basis events (e.g., earthquake conditions). A corrosion control program is in place to ensure that degradation of the steel does not impact the structural and leak integrity functions of these waste tanks. The SRS is currently retrieving waste from older waste tanks and processing the waste through the vitrification for long term stabilization. The retrieval processes prepare the tanks for ultimate closure (i.e., grouting) by removing sludge by mechanical and/or sluicing methods, dissolving salt cake by adding water, and chemical cleaning of the residual sludge with oxalic acid. Each of these retrieval methods will result in waste chemistry that does not meet the requirements of the current corrosion control program. Given the short-term exposure and limited remaining service life for the tanks in which retrievals are being performed, an assessment of the need for corrosion controls in these tanks was performed. The assessment reviewed the corrosion rates in the more aggressive environments and the postulated loads on the structure during the closure activities. The assessment concluded that the current corrosion control program may be suspended for a short period of time while final retrieval of the waste is performed. (authors)

  18. Diabetes Mellitus and Site-specific Colorectal Cancer Risk in Korea: A Case-control Study

    PubMed Central

    Lee, Jeonghee; Park, Sungchan

    2016-01-01

    Objectives: Previous large-scale cohort studies conducted in Korea have found a positive association between diabetes mellitus (DM) and colorectal cancer (CRC) in men only, in contrast to studies of other populations that have found significant associations in both men and women. Methods: A total of 1070 CRC cases and 2775 controls were recruited from the National Cancer Center, Korea between August 2010 and June 2013. Self-reported DM history and the duration of DM were compared between cases and controls. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated by binary and polytomous logistic regression models. Results: DM was associated with an elevated risk of CRC in both men (OR, 1.47; 95% CI, 1.13 to 1.90) and women (OR, 1.92; 95% CI, 1.24 to 2.98). This association remained when we controlled for age, body mass index, alcohol consumption, and physical activity level. In sub-site analyses, DM was associated with distal colon cancer risk in both men (multivariate OR, 2.04; 95% CI, 1.39 to 3.00) and women (multivariate ORs, 1.99; 95% CI, 1.05 to 3.79), while DM was only associated with rectal cancer risk in women (multivariate OR, 2.05; 95% CI, 1.10 to 3.82). No significant association was found between DM and proximal colon cancer risk in either men (multivariate OR, 1.45; 95% CI, 0.88 to 2.41) or women (multivariate OR, 1.79; 95% CI, 0.78 to 4.08). Conclusions: Overall, DM was associated with an increased risk of CRC in Koreans. However, potential over-estimation of the ORs should be considered due to potential biases from the case-control design. PMID:26841884

  19. EFFICACY OF BURNING, TILLAGE, AND BIOCIDES IN CONTROLLING BACTERIA RELEASED AT FIELD SITES AND EFFECTS ON INDIGENOUS BACTERIA AND FUNGI

    EPA Science Inventory

    The environmental use of genetically engineered microorganisms (GEMS) must include risk control methods that are effective and also safe to non-target organisms. n important component of risk control is the decontamination of field release sites of GEMS after their function is co...

  20. 77 FR 63873 - Johnson Controls, Inc. Including On-Site Leased Workers of Valley Staffing and AZ Quality Hudson...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-17

    ... notice of determination was published in the Federal Register on March 6, 2012 (77 FR 13354). The workers... Employment and Training Administration Johnson Controls, Inc. Including On-Site Leased Workers of Valley... Assistance on February 15, 2012, applicable to workers and former workers of Johnson Controls,...

  1. Exchange coupling between laterally adjacent nanomagnets.

    PubMed

    Dey, H; Csaba, G; Bernstein, G H; Porod, W

    2016-09-30

    We experimentally demonstrate exchange-coupling between laterally adjacent nanomagnets. Our results show that two neighboring nanomagnets that are each antiferromagnetically exchange-coupled to a common ferromagnetic bottom layer can be brought into strong ferromagnetic interaction. Simulations show that interlayer exchange coupling effectively promotes ferromagnetic alignment between the two nanomagnets, as opposed to antiferromagnetic alignment due to dipole-coupling. In order to experimentally demonstrate the proposed scheme, we fabricated arrays of pairs of elongated, single-domain nanomagnets. Magnetic force microscopy measurements show that most of the pairs are ferromagnetically ordered. The results are in agreement with micromagnetic simulations. The presented scheme can achieve coupling strengths that are significantly stronger than dipole coupling, potentially enabling far-reaching applications in Nanomagnet Logic, spin-wave devices and three-dimensional storage and computing. PMID:27535227

  2. Seismicity in Azerbaijan and Adjacent Caspian Sea

    SciTech Connect

    Panahi, Behrouz M.

    2006-03-23

    So far no general view on the geodynamic evolution of the Black Sea to the Caspian Sea region is elaborated. This is associated with the geological and structural complexities of the region revealed by geophysical, geochemical, petrologic, structural, and other studies. A clash of opinions on geodynamic conditions of the Caucasus region, sometimes mutually exclusive, can be explained by a simplified interpretation of the seismic data. In this paper I analyze available data on earthquake occurrences in Azerbaijan and the adjacent Caspian Sea region. The results of the analysis of macroseismic and instrumental data, seismic regime, and earthquake reoccurrence indicate that a level of seismicity in the region is moderate, and seismic event are concentrated in the shallow part of the lithosphere. Seismicity is mostly intra-plate, and spatial distribution of earthquake epicenters does not correlate with the plate boundaries.

  3. Boundary Layers of Air Adjacent to Cylinders

    PubMed Central

    Nobel, Park S.

    1974-01-01

    Using existing heat transfer data, a relatively simple expression was developed for estimating the effective thickness of the boundary layer of air surrounding cylinders. For wind velocities from 10 to 1000 cm/second, the calculated boundary-layer thickness agreed with that determined for water vapor diffusion from a moistened cylindrical surface 2 cm in diameter. It correctly predicted the resistance for water vapor movement across the boundary layers adjacent to the (cylindrical) inflorescence stems of Xanthorrhoea australis R. Br. and Scirpus validus Vahl and the leaves of Allium cepa L. The boundary-layer thickness decreased as the turbulence intensity increased. For a turbulence intensity representative of field conditions (0.5) and for νwindd between 200 and 30,000 cm2/second (where νwind is the mean wind velocity and d is the cylinder diameter), the effective boundary-layer thickness in centimeters was equal to [Formula: see text]. PMID:16658855

  4. Seismic responses of two adjacent buildings. II. Interaction

    USGS Publications Warehouse

    Celebi, Mehmet

    1993-01-01

    Presented in this part of the two-part paper is a study of the relations between earthquake motions recorded from two, adjacent, seven-story buildings, from a downhole below the foundation of one of the buildings and from three free-field sites, all within one city block. This unique data set was obtained during the Whittier-Narrows, Calif. earthquake of Oct. 1, 1987, Part I includes background information on the two buildings, the site, and the data set. Building response characteristics of a code-type instrumented building (A) and an extensively instrumented building (B) are also studied. In this part, spectral analysis techniques are used to study the relationships between the motions of the roofs and basements, the downhole and the free-field sites. It is asserted that there is building-soil-building interaction between the two buildings at a frequency of 2.35 Hz. Furthermore, the free-field motions are shown to be influenced by the presence of the buildings.

  5. Initialization of a spin qubit in a site-controlled nanowire quantum dot

    NASA Astrophysics Data System (ADS)

    Lagoudakis, Konstantinos G.; McMahon, Peter L.; Fischer, Kevin A.; Puri, Shruti; Müller, Kai; Dalacu, Dan; Poole, Philip J.; Reimer, Michael E.; Zwiller, Val; Yamamoto, Yoshihisa; Vučković, Jelena

    2016-05-01

    A fault-tolerant quantum repeater or quantum computer using solid-state spin-based quantum bits will likely require a physical implementation with many spins arranged in a grid. Self-assembled quantum dots (QDs) have been established as attractive candidates for building spin-based quantum information processing devices, but such QDs are randomly positioned, which makes them unsuitable for constructing large-scale processors. Recent efforts have shown that QDs embedded in nanowires can be deterministically positioned in regular arrays, can store single charges, and have excellent optical properties, but so far there have been no demonstrations of spin qubit operations using nanowire QDs. Here we demonstrate optical pumping of individual spins trapped in site-controlled nanowire QDs, resulting in high-fidelity spin-qubit initialization. This represents the next step towards establishing spins in nanowire QDs as quantum memories suitable for use in a large-scale, fault-tolerant quantum computer or repeater based on all-optical control of the spin qubits.

  6. Inositol phosphate pathway controls transcription of telomeric expression sites in trypanosomes.

    PubMed

    Cestari, Igor; Stuart, Ken

    2015-05-26

    African trypanosomes evade clearance by host antibodies by periodically changing their variant surface glycoprotein (VSG) coat. They transcribe only one VSG gene at a time from 1 of about 20 telomeric expression sites (ESs). They undergo antigenic variation by switching transcription between telomeric ESs or by recombination of the VSG gene expressed. We show that the inositol phosphate (IP) pathway controls transcription of telomeric ESs and VSG antigenic switching in Trypanosoma brucei. Conditional knockdown of phosphatidylinositol 5-kinase (TbPIP5K) or phosphatidylinositol 5-phosphatase (TbPIP5Pase) or overexpression of phospholipase C (TbPLC) derepresses numerous silent ESs in T. brucei bloodstream forms. The derepression is specific to telomeric ESs, and it coincides with an increase in the number of colocalizing telomeric and RNA polymerase I foci in the nucleus. Monoallelic VSG transcription resumes after reexpression of TbPIP5K; however, most of the resultant cells switched the VSG gene expressed. TbPIP5K, TbPLC, their substrates, and products localize to the plasma membrane, whereas TbPIP5Pase localizes to the nucleus proximal to telomeres. TbPIP5Pase associates with repressor/activator protein 1 (TbRAP1), and their telomeric silencing function is altered by TbPIP5K knockdown. These results show that specific steps in the IP pathway control ES transcription and antigenic switching in T. brucei by epigenetic regulation of telomere silencing. PMID:25964327

  7. Individual external exposures from Nevada Test Site fallout for Utah leukemia cases and controls.

    PubMed

    Lloyd, R D; Gren, D C; Simon, S L; Wrenn, M E; Hawthorne, H A; Lotz, T M; Stevens, W; Till, J E

    1990-11-01

    External gamma-ray exposures from fallout originating at the Nevada Test Site (NTS) have been assigned to 6,507 individual subjects (1,177 leukemia cases and 5,330 control subjects) who died as Utah residents between 1952 and 1981. Leukemia cases were identified, confirmed, and classified by cell type from the Utah Cancer Registry, Utah State vital records, and medical records. Residential histories were obtained from the Deceased Membership File (DMF) of the Church of Jesus Christ of Latter-day Saints (LDS), supplemented by information from the LDS Church Census Records that were taken in 1950, 1955, and 1960-62. Control subjects were selected randomly within age strata from the DMF and were frequency-matched to the cases by age at death and for sex. Individual radiation exposures were assigned as a function of residence location and time interval for each residence during the fallout period (1951-1958) using geographic exposure data taken from the literature. Temporal distribution of exposure for subjects who resided in more than one locality or who were born or died during the fallout period was determined from data of other investigators. Calculated gamma-ray exposures for each place of residence were summed for each subject to yield the exposure to fallout from the NTS. PMID:2211126

  8. Controlling the density and site of attachment of gold nanoparticles onto the surface of carbon nanotubes.

    PubMed

    Kumar, Suresh; Kaur, Inderpreet; Dharamvir, Keya; Bharadwaj, Lalit M

    2012-03-01

    A facile method for controlling the density and site of attachment of gold nanoparticles onto the surface of carbon nanotubes is demonstrated. Nitric acid based oxidation was carried out to create carboxylic groups exclusively at the ends of carbon nanotubes, whereas oxidation using a mixture of nitric and sulfuric acid with varied reaction time was carried out to control the population of carboxylic groups on the side walls of nanotubes. In turn, 4-aminothiophenol modified gold nanoparticles were covalently interfaced to these carboxylated multi-walled carbon nanotubes in the presence of a zero length cross-linker, 1-ethylene-3-(3-dimethylaminopropyl) carbodiimide. Raman spectroscopic results showed increase in height of disorder band with each of these successive steps, indicating the increase in degree of functionalization of the carbon nanotubes. Fourier transformed infrared spectroscopic analysis affirmed the functionalization of nanostructures and the formation of nanohybrid. Transmission electron and field emission scanning electron microscopic analysis ascertained the attachment of gold nanoparticles to the ends and side walls of the multi-walled carbon nanotubes. The new hybrid nanostructures may find applications in electronic, optoelectronic, and sensing devices. PMID:22218340

  9. Methane Flux in Cropland and Adjacent Riparian Buffers with Different Vegetation Covers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Conservation buffers established adjacent to cropped fields are widely promoted as off-site sinks for nonpoint source sediment and nutrients. These perennial plant systems have the potential to serve as sinks of methane (CH4) or may provide favorable conditions for CH4 production. This study quantif...

  10. DISTRIBUTION OF CONTAMINANTS IN WATERS OF MONROE HARBOR (RIVER RAISIN), MICHIGAN AND ADJACENT LAKE ERIE

    EPA Science Inventory

    The report describes the results of surveys of selected organochlorines and metals in Monroe Harbor and adjacent Lake Erie. Seasonal surveys at 3 sites of contaminant distribution in the water column were designed to support exposure effects studies and mass balance modeling. Che...

  11. 30 CFR 56.9103 - Clearance on adjacent tracks.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Clearance on adjacent tracks. 56.9103 Section..., Hauling, and Dumping Traffic Safety § 56.9103 Clearance on adjacent tracks. Railcars shall not be left on side tracks unless clearance is provided for traffic on adjacent tracks....

  12. 30 CFR 57.9103 - Clearance on adjacent tracks.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Clearance on adjacent tracks. 57.9103 Section..., Hauling, and Dumping Traffic Safety § 57.9103 Clearance on adjacent tracks. Railcars shall not be left on side tracks unless clearance is provided for traffic on adjacent tracks....

  13. 30 CFR 56.9103 - Clearance on adjacent tracks.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Clearance on adjacent tracks. 56.9103 Section..., Hauling, and Dumping Traffic Safety § 56.9103 Clearance on adjacent tracks. Railcars shall not be left on side tracks unless clearance is provided for traffic on adjacent tracks....

  14. 30 CFR 57.9103 - Clearance on adjacent tracks.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Clearance on adjacent tracks. 57.9103 Section..., Hauling, and Dumping Traffic Safety § 57.9103 Clearance on adjacent tracks. Railcars shall not be left on side tracks unless clearance is provided for traffic on adjacent tracks....

  15. 33 CFR 80.1395 - Puget Sound and adjacent waters.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Puget Sound and adjacent waters... INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Thirteenth District § 80.1395 Puget Sound and adjacent waters. The 72 COLREGS shall apply on all waters of Puget Sound and adjacent waters, including Lake...

  16. 33 CFR 80.1395 - Puget Sound and adjacent waters.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Puget Sound and adjacent waters... INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Thirteenth District § 80.1395 Puget Sound and adjacent waters. The 72 COLREGS shall apply on all waters of Puget Sound and adjacent waters, including Lake...

  17. 33 CFR 80.1395 - Puget Sound and adjacent waters.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Puget Sound and adjacent waters... INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Thirteenth District § 80.1395 Puget Sound and adjacent waters. The 72 COLREGS shall apply on all waters of Puget Sound and adjacent waters, including Lake...

  18. 33 CFR 80.1395 - Puget Sound and adjacent waters.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Puget Sound and adjacent waters... INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Thirteenth District § 80.1395 Puget Sound and adjacent waters. The 72 COLREGS shall apply on all waters of Puget Sound and adjacent waters, including Lake...

  19. 33 CFR 80.1395 - Puget Sound and adjacent waters.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Puget Sound and adjacent waters... INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Thirteenth District § 80.1395 Puget Sound and adjacent waters. The 72 COLREGS shall apply on all waters of Puget Sound and adjacent waters, including Lake...

  20. A socio-ecological adaptive approach to contaminated mega-site management: From 'control and correct' to 'coping with change'

    NASA Astrophysics Data System (ADS)

    Schirmer, Mario; Lyon, Ken; Armstrong, James E.; Farrell, Katharine N.

    2012-01-01

    Mega-sites have a notable impact on surrounding ecological systems. At such sites there are substantial risks associated with complex socio-ecological interactions that are hard to characterize, let alone model and predict. While the urge to control and clean-up mega-sites (control and correct) is understandable, rather than setting a goal of cleaning up such sites, we suggest a more realistic response strategy is to address these massive and persistent sources of contamination by acknowledging their position as new features of the socio-ecological landscapes within which they are located. As it seems nearly impossible to clean up such sites, we argue for consideration of a 'coping with change' rather than a 'control and correct' approach. This strategy recognizes that the current management option for a mega-site, in light of its physical complexities and due to changing societal preferences, geochemical transformations, hydrogeology knowledge and remedial technology options may not remain optimal in future, and therefore needs to be continuously adapted, as community, ecology, technology and understanding change over time. This approach creates an opportunity to consider the relationship between a mega-site and its human and ecological environments in a different and more dynamic way. Our proposed approach relies on iterative adaptive management to incorporate mega-site management into the overall socio-ecological systems of the site's context. This approach effectively embeds mega-site management planning in a triple bottom line and environmental sustainability structure, rather than simply using single measures of success, such as contaminant-based guidelines. Recognizing that there is probably no best solution for managing a mega-site, we present a starting point for engaging constructively with this seemingly intractable issue. Therefore, we aim to initiate discussion about a new approach to mega-site management, in which the complexity of the problems posed

  1. Rate of hydrolysis in ATP synthase is fine-tuned by α-subunit motif controlling active site conformation.

    PubMed

    Beke-Somfai, Tamás; Lincoln, Per; Nordén, Bengt

    2013-02-01

    Computer-designed artificial enzymes will require precise understanding of how conformation of active sites may control barrier heights of key transition states, including dependence on structure and dynamics at larger molecular scale. F(o)F(1) ATP synthase is interesting as a model system: a delicate molecular machine synthesizing or hydrolyzing ATP using a rotary motor. Isolated F(1) performs hydrolysis with a rate very sensitive to ATP concentration. Experimental and theoretical results show that, at low ATP concentrations, ATP is slowly hydrolyzed in the so-called tight binding site, whereas at higher concentrations, the binding of additional ATP molecules induces rotation of the central γ-subunit, thereby forcing the site to transform through subtle conformational changes into a loose binding site in which hydrolysis occurs faster. How the 1-Å-scale rearrangements are controlled is not yet fully understood. By a combination of theoretical approaches, we address how large macromolecular rearrangements may manipulate the active site and how the reaction rate changes with active site conformation. Simulations reveal that, in response to γ-subunit position, the active site conformation is fine-tuned mainly by small α-subunit changes. Quantum mechanics-based results confirm that the sub-Ångström gradual changes between tight and loose binding site structures dramatically alter the hydrolysis rate. PMID:23345443

  2. Toward efficient nanoporous catalysts: controlling site-isolation and concentration of grafted catalytic sites on nanoporous materials with solvents and colorimetric elucidation of their site-isolation.

    PubMed

    Sharma, Krishna K; Anan, Abhishek; Buckley, Robert P; Ouellette, Wayne; Asefa, Tewodros

    2008-01-01

    We report that the polarity and dielectric constants of solvents used for grafting organosilanes on mesoporous materials strongly affect the concentration of grafted organic groups, the degree of their site-isolation, and the catalytic properties of the resulting materials. Polar and nonpolar organosilanes as well as polar-protic, dipolar-aprotic, and nonpolar solvents were investigated. Polar-protic solvents, which have high dielectric constants, resulted in smaller concentrations ( approximately 1-2 mmol/g) of polar organic groups such as 3-aminopropyl groups, higher surface area materials, site-isolated organic groups, and more efficient catalytic properties toward the Henry reaction of p-hydroxybenzaldehyde with nitromethane. On the other hand, dipolar-aprotic and nonpolar solvents resulted in larger concentrations ( approximately 2-3 mmol/g) of grafted polar functional groups, lower-to-higher surface area materials, more densely populated catalytic groups, and poor-to-efficient catalytic properties toward the Henry reaction. Both the polar-protic and dipolar-aprotic solvents resulted in significantly lower concentration of grafted groups for nonpolar organosilanes such as (3-mercaptopropyl)trimethoxysilane compared to corresponding grafting of the polar amino-organosilanes. The relationship between the solvent properties and the percentage and degree of site-isolation of the grafted functional groups was attributed to differences in solvation of the organosilanes and silanols in various solvents and possible hydrogen-bonding between the organsilanes and the solvents. The degree of site-isolation of the amine groups, which affect the material's catalytic properties, was elucidated by a new colorimetric method involving probing of the absorption maxima (lambdamax) on the d-d electronic spectrum of Cu2+ complexes with the amine-functionalized materials and the colors of the samples. The absorption lambdamax and the colors of the materials were found to be

  3. Comparison of mosquito control programs in seven urban sites in Africa, the Middle East, and the Americas

    PubMed Central

    Impoinvil, Daniel E.; Ahmad, Sajjad; Troyo, Adriana; Keating, Joseph; Githeko, Andrew K.; Mbogo, Charles M; Kibe, Lydiah; Githure, John I.; Gad, Adel M.; Hassan, Ali N.; Orshan, Laor; Warburg, Alon; Calderón-Arguedas, Olger; Sánchez-Loría, Victoria M.; Velit-Suarez, Rosanna; Chadee, Dave D.; Novak, Robert J.; Beier, John C.

    2007-01-01

    Mosquito control programs at seven urban sites in Kenya, Egypt, Israel, Costa Rica, and Trinidad are described and compared. Site-specific urban and disease characteristics, organizational diagrams, and strengths, weaknesses, obstacles and threats (SWOT) analysis tools are used to provide a descriptive assessment of each mosquito control program, and provide a comparison of the factors affecting mosquito abatement. The information for SWOT analysis is collected from surveys, focus group discussions, and personal communication. SWOT analysis identified various issues affecting the efficiency and sustainability of mosquito control operations. The main outcome of our work was the description and comparison of mosquito control operations within the context of each study site’s biological, social, political, management, and economic conditions. The issues identified in this study ranged from lack of inter-sector collaboration to operational issues of mosquito control efforts. A lack of sustainable funding for mosquito control was a common problem for most sites. Many unique problems were also identified, which included lack of mosquito surveillance, lack of law enforcement, and negative consequences of human behavior. Identifying common virtues and shortcomings of mosquito control operations is useful in identifying “best practices” for mosquito control operations, thus leading to better control of mosquito biting and mosquito-borne disease transmission. PMID:17316882

  4. Elevational and Latitudinal Gradient Sites Enable Phenology Controls and Climate Change Detection

    NASA Astrophysics Data System (ADS)

    Losleben, M. V.; Weltzin, J. F.; Billick, I.; Jones, D.

    2008-12-01

    Phenology is the study of the timing of recurring biological phases, the causes of their timing with regard to biotic and abiotic forces, and the interrelation among phases of the same or different species. Although phenology is a far-reaching component of environmental science, it is poorly understood relative to other ecological patterns and processes. For example, it is unclear how climatic attributes affect the phenology of different organisms, and how those attributes vary in importance on different spatial and temporal scales. We know phenology affects the abundance and diversity of organisms, and their function and interactions in the environment, especially their effects on fluxes in water, energy, and chemical elements at various scales. With sufficient observations and understanding, phenology can be used as a predictor for other processes and variables of importance at local to global scales, and phenology could drive a variety of ecological forecast models with both scientific and practical applications. Integration of spatially-extensive phenological data and models with both short and long-term climatic forecasts offer a powerful agent for human adaptation to ongoing and future climate change. To fully utilize the value of phenology, not only more observations at more locations are needed, but also linkages between climatic factors and phenology must be more firmly established through linked direct observations and remotely sensed (Landscape Phenology or LSP) measurements with climatic factors. Sites along elevational gradients over a range of latitudes provide this opportunity. Elevational gradients present the phenological observational efficiency of compressed ecosystem transitions, and through climatic matching of sites, latitudinal range presents opportunities to control for additional abiotic factors such as day length, seasonal variability, storm track, and atmospheric chemistry. These sites also provide excellent platforms to advance new, and

  5. On the nature of the optimal control problem at leaking underground fuel tank sites

    SciTech Connect

    McDowell, B

    1998-12-01

    In California, leaking underground fuel tank (LUFT) legislation was conceived because of concern that ''time bomb plumes'' would ultimately impact a significant portion of the state's ground and surface water resources. However, it has been found that fuel hydrocarbons (FHC) plumes are stable at relatively short distances from the source in areas of shallow groundwater. In urban areas, these shallow aquifers are not even recommended for use because they are subject to contamination from sewers, storm drains, septic fields and a variety of other sources. After the FHC source has been removed, risk to human health or the environment is insignificant in most cases. For this reason, cleanup to maximum contaminant levels (MCLs) will not significantly reduce the social damages associated with current or near-term human health or ecological risk. Based on these findings, California would be able to save significant resources that had been allocated for LUFT-site cleanup. Non-convexities in the rate of decay function and non-differentiability in the cleanup and social damage functions appear to limit the usefulness of models, such as Caputo and Wilen's (1995), that attempt to characterize the optimal cleanup path using marginal analyses. Furthermore, the effect of active remediation efforts on the natural rate of decay in stable plumes is not taken into account in their model. The imposition of deed restrictions prior to a demonstration of cleanup to MCLs is an additional conservative measure imposed by the California Regional Water Quality Control Boards (RWQCBs) to reduce the uncertainty associated with health risks to future users. These measures impose costs on society in the form of lost rents that have not been considered by regulators. By estimating the differential rents during the time to cleanup, regulators would be able to compare the costs of imposing deed restrictions with the values that society imparts to protection of future users. Both land and water

  6. Roof Deformation, Failure Characteristics, and Preventive Techniques of Gob-Side Entry Driving Heading Adjacent to the Advancing Working Face

    NASA Astrophysics Data System (ADS)

    Bai, Jian-biao; Shen, Wen-long; Guo, Guan-long; Wang, Xiang-yu; Yu, Yang

    2015-11-01

    In mining excavation, the roof bending subsidence of gob-side entry driving heading adjacent to the advancing working face (HAWF) can be considerable. Influenced by the original rock pressure, the front and lateral abutment pressure of the adjacent working face, and the front abutment pressure of the current working face, the support body can easily fail, leading to serious instability of the rock mass surrounding the tunnel. To study the stress state and the deformation failure mechanism of the HAWF roof structure, we use on-site survey data, numerical simulation, and theoretical calculations to fit the spatial distribution law of mining abutment pressure piecewise, and establish a dynamic mechanical model of the roof structure. We then propose a roof failure criterion and examine the roof flexure deformation behavioral pattern. We found that the central part of the roof is the main point that controls the surrounding rock. To prevent the deformation and collapse of the roof and rock surrounding the tunnel, we propose techniques that can be applied to HAWF gob-side entry driving, including setting the coal pillar width, the driving stop and restart timing, and other control concepts.

  7. A Multifaceted Sampling Approach to Better Understanding Biogeochemical and Hydrogeological Controls on Uranium Mobility at a Former Uranium Mill Tailings Site in Riverton, Wyoming

    NASA Astrophysics Data System (ADS)

    Dam, W. L.; Johnson, R. H.; Campbell, S.; Bone, S. E.; Noel, V.; Bargar, J.

    2015-12-01

    Understanding uranium mobility in subsurface environments is not trivial. Obtaining sufficient data to accurately represent soil and aquifer characteristics can require unique approaches that evolve with added site knowledge. At Riverton, the primary source of uranium mill tailings remaining from ore processing was removed but contaminant plumes have persisted longer than predicted by groundwater modeling. What are the primary mechanisms controlling plume persistence? DOE is conducting new characterization studies to assist our understanding of underlying biogeochemical and hydrogeological mechanisms affecting secondary sources. A variety of field sampling techniques are being sequentially employed including augering, trenching, pore water sampling, and installing multi-level wells. In August 2012, vadose zone soil samples from 34 locations and groundwater from 103 boreholes were collected with Geoprobe ® direct push rods. Lower than expected uranium concentrations in composited shallow soils indicated the need for more focused and deeper samples. In May 2014, soil samples containing evaporites were collected along the bank of the Little Wind River; elevated uranium concentrations in evaporite minerals correlated with plume configurations and reflect contaminated groundwater discharge at the river. In September 2014, hand anger samples collected by the river and oxbow lake also indicated the presence of organic rich zones containing elevated uranium (>50 mg/kg). Subsequent samples collected from five backhoe trenches in May 2015 revealed a highly heterogeneous vadose zone composed of clay, silt, sand and cobbles containing evaporites and organic rich zones which may interact with groundwater plumes.Plans for August 2015 include sonic drilling to obtain continuous cores from the surface down to the base of the surficial aquifer with multi-level monitoring wells constructed in each borehole to assess vertical variation in groundwater chemistry. Temporary well

  8. Does Metaphyseal Cement Augmentation in Fracture Management Influence the Adjacent Subchondral Bone and Joint Cartilage?

    PubMed Central

    Goetzen, Michael; Hofmann-Fliri, Ladina; Arens, Daniel; Zeiter, Stephan; Stadelmann, Vincent; Nehrbass, Dirk; Richards, R. Geoff; Blauth, Michael

    2015-01-01

    Abstract Augmentation of implants with polymethylmethacrylate (PMMA) bone cement in osteoporotic fractures is a promising approach to increase implant purchase. Side effects of PMMA for the metaphyseal bone, particularly for the adjacent subchondral bone plate and joint cartilage, have not yet been studied. The following experimental study investigates whether subchondral PMMA injection compromises the homeostasis of the subchondral bone and/or the joint cartilage. Ten mature sheep were used to simulate subchondral PMMA injection. Follow-ups of 2 (4 animals) and 4 (6 animals) months were chosen to investigate possible cartilage damage and subchondral plate alterations in the knee. Evaluation was completed by means of high-resolution peripheral quantitative computed tomography (HRpQCT) imaging, histopathological osteoarthritis scoring, and determination of glycosaminoglycan content in the joint cartilage. Results were compared with the untreated contralateral knee and statistically analyzed using nonparametric tests. Evaluation of the histological osteoarthritis score revealed no obvious cartilage damage for the treated knee; median histological score after 2 months 0 (range 4), after 4 months 1 (range 5). There was no significant difference when compared with the untreated control site after 2 and 4 months (P = 0.23 and 0.76, respectively). HRpQCT imaging showed no damage to the metaphyseal trabeculae. Glycosaminoglycan measurements of the treated joint cartilage after 4 months revealed no significant difference compared with the untreated cartilage (P = 0.24). The findings of this study support initial clinical observation that PMMA implant augmentation of metaphyseal fractures appears to be a safe procedure for fixation without harming the subchondral bone plate and adjacent joint cartilage. PMID:25621690

  9. Corrective measures technology for shallow land burial at arid sites: field studies of biointrusion barriers and erosion control

    SciTech Connect

    Nyhan, J.W.; Hakonson, T.E.; Lopez, E.A.

    1986-03-01

    The field research program involving corrective measures technologies for arid shallow land burial (SLB) sites is described. Results of field testing of a biointrusion barrier installed at a close-out waste disposal site (Area B) at Los Alamos are presented. Soil erosion and infiltration of water into a simulated trench cap with various surface treatments were measured, and the interaction between erosion control and subsurface water dynamics is discussed relative to waste management.

  10. Geological controls on the performance of CO2 injection and storage sites

    NASA Astrophysics Data System (ADS)

    Ringrose, P. S.; Nazarian, B.; Gemmer, L.; Cavanagh, A.

    2011-12-01

    Using experience gained from several pioneering industrial-scale CO2 storage projects we identify the key factors controlling injection performance, both for the injection period (c. 10 years) and for the longer term (>10 years). The Sleipner, In Salah and Snøhvit storage sites, which together have succeeded in disposing of over 16 Mt of CO2, present highly variable reservoirs, covering a range of reservoir depth, permeability and geological architecture. Reservoir modelling studies show how CO2 plume development has been controlled by geology, geo-mechanics and fluid phase behaviour. Three general themes about CO2 in the subsurface emerge from these cases studies: a) Geology: Variations in geological architecture result in highly non-uniform plume development. Short-term and near-well injection performance is viscous dominated, while longer-term and far-field performance becomes gravity/capillary dominated. The case studies illustrate how thin-shale layers, top-reservoir topography, faults and fractures control actual CO2 plume development. Understanding how these controls affect field performance is best achieved by careful tuning of reservoir models to observations from reservoir monitoring. b) Rock mechanics: Geo-mechanical properties affect storage capacity, limit well injectivity and determine pressure development within the reservoir. Simple linear elastic models are generally insufficient, whereas by using detailed mechanical models we have been able to fine-tune the reservoir and overburden mechanical models to learn how the rock system responds to fluid pressure. c) Phase behaviour: While pure CO2 phase behaviour is well understood, the behaviour of gas mixtures, within the inherently uncertain ranges of in situ reservoir pressure and temperature, presents a significant challenge, leading to large uncertainties in gas density, viscosity, and solubility. Detailed models of near-wellbore multi-phase flow show how injectivity and plume growth around the

  11. Robotic single site versus robotic multiport hysterectomy in early endometrial cancer: a case control study

    PubMed Central

    2016-01-01

    Objective To compare surgical outcomes and cost of robotic single-site hysterectomy (RSSH) versus robotic multiport hysterectomy (RMPH) in early stage endometrial cancer. Methods This is a retrospective case-control study, comparing perioperative outcomes and costs of RSSH and RMPH in early stage endometrial cancer patients. RSSH were matched 1:2 according to age, body mass index, comorbidity, the International Federation of Gynecology and Obstetric (FIGO) stage, type of radical surgery, histologic type, and grading. Mean hospital cost per discharge was calculated summarizing the cost of daily hospital room charges, operating room, cost of supplies and length of hospital stay. Results A total of 23 women who underwent RSSH were matched with 46 historic controls treated by RMPH in the same institute, with the same surgical team. No significant differences were found in terms of age, histologic type, stage, and grading. Operative time was similar: 102.5 minutes in RMPH and 110 in RSSH (p=0.889). Blood loss was lower in RSSH than in RMPH (respectively, 50 mL vs. 100 mL, p=0.001). Hospital stay was 3 days in RMPH and 2 days in RSSH (p=0.001). No intraoperative complications occurred in both groups. Early postoperative complications were 2.2% in RMPH and 4.3% in RSSH. Overall cost was higher in RMPH than in RSSH (respectively, $7,772.15 vs. $5,181.06). Conclusion Our retrospective study suggests the safety and feasibility of RSSH for staging early endometrial cancer without major differences from the RMPH in terms of surgical outcomes, but with lower hospital costs. Certainly, further studies are eagerly warranted to confirm our findings. PMID:27171672

  12. Hydrodynamic parameters estimation from self-potential data in a controlled full scale site

    NASA Astrophysics Data System (ADS)

    Chidichimo, Francesco; De Biase, Michele; Rizzo, Enzo; Masi, Salvatore; Straface, Salvatore

    2015-03-01

    A multi-physical approach developed for the hydrodynamic characterization of porous media using hydrogeophysical information is presented. Several pumping tests were performed in the Hydrogeosite Laboratory, a controlled full-scale site designed and constructed at the CNR-IMAA (Consiglio Nazionale delle Ricerche - Istituto di Metodologia per l'Analisi Ambientale), in Marsico Nuovo (Basilicata Region, Southern Italy), in order to obtain an intermediate stage between laboratory experiments and field survey. The facility consists of a pool, used to study water infiltration processes, to simulate the space and time dynamics of subsurface contamination phenomena, to improve and to find new relationship between geophysical and hydrogeological parameters, to test and to calibrate new geophysical techniques and instruments. Therefore, the Hydrogeosite Laboratory has the advantage of carrying out controlled experiments, like in a flow cell or sandbox, but at field comparable scale. The data collected during the experiments have been used to estimate the saturated hydraulic conductivity ks [ms-1] using a coupled inversion model working in transient conditions, made up of the modified Richards equation describing the water flow in a variably saturated porous medium and the Poisson equation providing the self-potential ϕ [V], which naturally occurs at points of the soil surface owing to the presence of an electric field produced by the motion of underground electrolytic fluids through porous systems. The result obtained by this multi-physical numerical approach, which removes all the approximations adopted in previous works, makes a useful instrument for real heterogeneous aquifer characterization and for predictive analysis of its behavior.

  13. Interaction between adjacent lightning discharges in clouds

    NASA Astrophysics Data System (ADS)

    Wang, Yanhui; Zhang, Guangshu; Zhang, Tong; Li, Yajun; Wu, Bin; Zhang, Tinglong

    2013-07-01

    Using a 3D lightning radiation source locating system (LLS), three pairs of associated lightning discharges (two or more adjacent lightning discharges following an arbitrary rule that their space-gap was less than 10 km and their time-gap was less than 800 ms) were observed, and the interaction between associated lightning discharges was analyzed. All these three pairs of associated lightning discharges were found to involve three or more charge regions (the ground was considered as a special charge region). Moreover, at least one charge region involved two lightning discharges per pair of associated lightning discharges. Identified from electric field changes, the subsequent lightning discharges were suppressed by the prior lightning discharges. However, it is possible that the prior lightning discharge provided a remaining discharge channel to facilitate the subsequent lightning discharge. The third case provided evidence of this possibility. Together, the results suggested that, if the charges in the main negative charge region can be consumed using artificial lightning above the main negative charge regions, lightning accidents on the ground could be greatly reduced, on the condition that the height of the main negative charge region and the charge intensity of the lower positive charge region are suitable.

  14. Exciton dynamics in a site-controlled quantum dot coupled to a photonic crystal cavity

    SciTech Connect

    Jarlov, C. Lyasota, A.; Ferrier, L.; Gallo, P.; Dwir, B.; Rudra, A.; Kapon, E.

    2015-11-09

    Exciton and cavity mode (CM) dynamics in site-controlled pyramidal quantum dots (QDs), integrated with linear photonic crystal membrane cavities, are investigated for a range of temperatures and photo-excitation power levels. The absence of spurious multi-excitonic effects, normally observed in similar structures based on self-assembled QDs, permits the observation of effects intrinsic to two-level systems embedded in a solid state matrix and interacting with optical cavity modes. The coupled exciton and CM dynamics follow the same trend, indicating that the CM is fed only by the exciton transition. The Purcell reduction of the QD and CM decay times is reproduced well by a theoretical model that includes exciton linewidth broadening and temperature dependent non-radiative processes, from which we extract a Purcell factor of 17 ± 5. For excitation powers above QD saturation, we show the influence of quantum wire barrier states at short delay time, and demonstrate the absence of multiexcitonic background emission.

  15. Narrow optical line width from site-controlled InGaAs quantum dots

    NASA Astrophysics Data System (ADS)

    Yang, Lily; Yakes, Michael; Sweeney, Timothy; Carter, Samuel; Kim, Chulsoo; Kim, Mijin; Bracker, Allan; Gammon, Daniel

    2013-03-01

    The incorporation of self-assembled quantum dots (QDs) in systematically scalable quantum devices requires a method of nucleating dots with nanometer-scale spatial accuracy while preserving their narrow optical line width. We have developed a technique combining e-beam lithography, wet etching, and molecular beam epitaxial (MBE) growth to deterministically position InGaAs QDs with spectrometer limited photoluminescence line widths. Our technique takes advantage of the anisotropy in GaAs growth to evolve an etched pattern of holes and lines into faceted structures in which dots nucleate. Using this technique, we were able to grow a buffer layer of pure GaAs up to 90 nm in thickness between the processed surface and the dot nucleation surface, effectively separating the QDs from unavoidable residual defects and impurities on the patterned surface that broaden their optical line widths. Additionally, we demonstrate control over the number of dots nucleating per site, from single to a chain of several, by varying the dimensions of the original pattern. Our dots are grown in a Schottky diode structure. Their PL spectrum shows discrete charging transitions, with narrow linewidths near the spectrometer's resolution limit of 20 micro eV.

  16. Quality control of transcription start site selection by nonsense-mediated-mRNA decay.

    PubMed

    Malabat, Christophe; Feuerbach, Frank; Ma, Laurence; Saveanu, Cosmin; Jacquier, Alain

    2015-01-01

    Nonsense-mediated mRNA decay (NMD) is a translation-dependent RNA quality-control pathway targeting transcripts such as messenger RNAs harboring premature stop-codons or short upstream open reading frame (uORFs). Our transcription start sites (TSSs) analysis of Saccharomyces cerevisiae cells deficient for RNA degradation pathways revealed that about half of the pervasive transcripts are degraded by NMD, which provides a fail-safe mechanism to remove spurious transcripts that escaped degradation in the nucleus. Moreover, we found that the low specificity of RNA polymerase II TSSs selection generates, for 47% of the expressed genes, NMD-sensitive transcript isoforms carrying uORFs or starting downstream of the ATG START codon. Despite the low abundance of this last category of isoforms, their presence seems to constrain genomic sequences, as suggested by the significant bias against in-frame ATGs specifically found at the beginning of the corresponding genes and reflected by a depletion of methionines in the N-terminus of the encoded proteins. PMID:25905671

  17. Site-specific phosphorylation and microtubule dynamics control Pyrin inflammasome activation.

    PubMed

    Gao, Wenqing; Yang, Jieling; Liu, Wang; Wang, Yupeng; Shao, Feng

    2016-08-16

    Pyrin, encoded by the MEFV gene, is best known for its gain-of-function mutations causing familial Mediterranean fever (FMF), an autoinflammatory disease. Pyrin forms a caspase-1-activating inflammasome in response to inactivating modifications of Rho GTPases by various bacterial toxins or effectors. Pyrin-mediated innate immunity is unique in that it senses bacterial virulence rather than microbial molecules, but its mechanism of activation is unknown. Here we show that Pyrin was phosphorylated in bone marrow-derived macrophages and dendritic cells. We identified Ser-205 and Ser-241 in mouse Pyrin whose phosphorylation resulted in inhibitory binding by cellular 14-3-3 proteins. The two serines underwent dephosphorylation upon toxin stimulation or bacterial infection, triggering 14-3-3 dissociation, which correlated with Pyrin inflammasome activation. We developed antibodies specific for phosphorylated Ser-205 and Ser-241, which confirmed the stimuli-induced dephosphorylation of endogenous Pyrin. Mutational analyses indicated that both phosphorylation and signal-induced dephosphorylation of Ser-205/241 are important for Pyrin activation. Moreover, microtubule drugs, including colchicine, commonly used to treat FMF, effectively blocked activation of the Pyrin inflammasome. These drugs did not affect Pyrin dephosphorylation and 14-3-3 dissociation but inhibited Pyrin-mediated apoptosis-associated Speck-like protein containing CARD (ASC) aggregation. Our study reveals that site-specific (de)phosphorylation and microtubule dynamics critically control Pyrin inflammasome activation, illustrating a fine and complex mechanism in cytosolic immunity. PMID:27482109

  18. On-site treatment and landfilling of MSWI air pollution control residues.

    PubMed

    Lundtorp, K; Jensen, D L; Sørensen, M A; Mosbaek, H; Christensen, T H

    2003-02-28

    Air pollution control (APC) residues from municipal solid waste incineration (MSWI) are difficult to landfill due to substantial leaching of trace metals. An on-site pretreatment prior to landfilling of APC-residues was investigated in terms of bench-scale experiments with a semidry APC-residue and a fly ash. The treatment involved mixing of the residues with a ferrous sulphate solution and subsequent oxidation of the suspension. Afterwards, the suspension was spread on a dedicated landfill section and allowed to drain by gravity through the drainage system of the landfill. The wastewater from the process, collected through the drainage system, contained large concentrations of salts (Cl: 14-30 g/l, Na: 4-9 g/l, K: 5-11 g/l, Ca: 2-12 g/l) but low concentrations of trace metals (e.g. Pb: 14-100 microg/l, Cd: <2-7 microg/l). The treated residues left in the landfills were later subject to leaching by simulated rainfall. The leachate contained low concentrations of trace metals (Pb: <120 microg/l, Cd: <2 microg/l, Cr: <485 microg/l). The leachate concentrations from the treated APC-residues were substantially reduced compared to concentrations in leachate from untreated APC-residues. Particularly in the early stages of the leaching, concentrations of trace metals were reduced by up to four orders of magnitude. PMID:12573829

  19. Classical Hodgkin Lymphoma Arising Adjacent to a Breast Implant.

    PubMed

    Ryan, Ciara; Ged, Yasser; Quinn, Fiona; Walker, Jan; Kennedy, John; Gillham, Charles; Pittaluga, Stefania; McDermott, Ronan; Vandenberghe, Elisabeth; Grant, Cliona; Flavin, Richard

    2016-08-01

    Breast implant-associated lymphoma has recently gained wide recognition. Anaplastic large cell lymphoma (ALCL) is the most frequently diagnosed subtype in this setting but the spectrum is broadening. A 66-year-old woman developed swelling and itch around her saline implant 6 years after its insertion. Imaging revealed a fluid collection surrounding the implant with an adjacent mass. Microscopy showed sclerotic tissue punctuated by discrete cellular nodules comprising small lymphocytes, eosinophils and interspersed large atypical Hodgkin Reed-Sternberg (HRS)-like cells. The HRS-like cells stained positively for CD30 and CD15 by immunohistochemistry. Small T-lymphocytes formed rosettes around HRS-like cells. Appearances were consistent with classical Hodgkin lymphoma (HL). Multiplex polymerase chain reaction demonstrated no clonal rearrangements of immunoglobulin or T-cell receptor genes, however, a t(14;18)(q32;q21)BCL2-JH translocation involving the major breakpoint region of the bcl2 gene was present. Staging positron emission tomography-computed tomography scan revealed FDG-avid masses in the right axilla and pelvis. Subsequent pathological examination identified low-grade follicular lymphoma (FL) with a t(14;18) translocation at these sites. To our knowledge, this is the first case of HL arising adjacent to a breast implant. An awareness of this diagnosis is important as classical HL, with its prominent mixed inflammatory background, may be overlooked as a reactive process when histologically assessing capsulectomy specimens. It is also important in the differential diagnosis for implant-associated ALCL as both contain large atypical CD30-positive cells highlighting the need for full immunohistochemical and molecular workup in such cases. This case also adds to the large body of literature regarding the association between HL and FL. PMID:26888955

  20. Seismic responses of two adjacent buildings. I. Data and analyses

    USGS Publications Warehouse

    Celebi, Mehmet

    1993-01-01

    In this two-part paper, responses of two, adjacent, seven-story buildings in Norwalk, California, to the Whittier-Narrows, Calif, earthquake of Oct. 1, 1987 are studied. Building A, instrumented according to code recommendations, and building B, extensively instrumented, are offset by 16.3 m from one another. The data set includes motions from the superstructure of both buildings, from a downhole below the foundation of building B, and from three free-field sites. Part I of the paper includes descriptions of the buildings, site, instrumentation, and analysis of the data of each building. System identification and spectral analysis techniques are employed in part I. Building A has identical first-mode frequencies of 0.65 Hz for both building axes. The strong-motion response characteristics of building A are considerably different than those determined from low-amplitude tests. Building B has fundamental modes at 0.76 Hz and 0.83 Hz in the major and minor axes, respectively. Torsional and diaphragm effects in building B are negligible.

  1. Site-controlled InGaAs/GaAs pyramidal quantum dots grown by MOVPE on patterned substrates using triethylgallium

    NASA Astrophysics Data System (ADS)

    Rigal, B.; Jarlov, C.; Rudra, A.; Gallo, P.; Lyasota, A.; Dwir, B.; Kapon, E.

    2015-03-01

    The fabrication and characterization of site-controlled InGaAs/GaAs quantum dots (QDs) made by MOVPE using triethylgallium (TEGa) on patterned {1 1 1}B GaAs substrates are reported. Results are compared to more traditional pyramidal QD structures grown employing trimethylgallium (TMGa). Several potential advantages of the use of TEGa are demonstrated, including more reproducible achievement of QD transitions with narrow (<100 μeV) linewidths and better spectral uniformity across patterned substrates. These features are important for QD integration in optical cavities, which require both site and spectral stringent control.

  2. Factors controlling building susceptibility to earthquakes: 14-year recordings of Islamic archaeological sites in Old Cairo, Egypt: a case study

    NASA Astrophysics Data System (ADS)

    Kamh, G. M. E.; Kallash, A.; Azzam, R.

    2008-11-01

    Cairo City has a large number and different forms of Islamic archaeological sites, in particular, at El-Gammalia and El-Moez streets, as well as Coptic archaeological sites, e.g. at Mari Gergis. Human interference and activities at these historical areas resulted in flooding such sites’ foundations with domestic water, deteriorating its basal courses by salt weathering. The 1992 earthquake is another natural environmental hazard severely affecting many of these sites. The aim of the current study is to examine some factors (of bedrock and buildings) that are expected to control building susceptibility to damage by earthquakes by taking 38 Islamic archaeological sites in the El-Gammalia area as a representative case study. Detailed field recordings of site damage category before and after the quake and continued recording of damage features generated by the 1992 quake over the last 14 years, measuring depth to sub-surface water, measuring buildings’ height before the quake and bedrock sampling at these sites for geotechnical investigations were all considered for achieving this aim. The data has been processed mathematically and graphically (using the Excel package) to examine the main factors responsible for building susceptibility to damage by earthquakes. The selected archaeological sites give an excellent representation of the factors controlling building susceptibility to damage by quakes; it is found that the sites with heights (before the quake) ranging from 12 to 14 m are the most affected ones; the sites with the highest damage category before the quake were more susceptible to more damage by the quake; the sites that had been built on alluvium soil were more affected than those built on the Eocene limestone. The age of these sites has, to a small extent, indirect control on sites’ susceptibility to damage by the quake, particularly in parts flooded with domestic water (i.e. affected by salt weathering). The depth to sub-surface water is an effective

  3. Petroleum basins of Sakhalin and adjacent shelf

    SciTech Connect

    Mavrinski, Y.; Koblov, E. )

    1993-09-01

    Sixty-seven oil and gas fields have been discovered on Sakhalin and the adjacent shelf but the distribution of fields is uneven in north Sakhalin, south Sakhalin, and the Tatar basins. The sedimentary cover is composed of sandy, clayey, and siliceous rocks, with volcanogenic and coal-bearing deposits of Upper Cretaceous, Paleogene, and Neogene 8-12 km thick. Marine clayey and siliceous oil source rocks are regionally developed in the section at different stratigraphic levels; the organic matter is of mixed type and the content varies from 0.5 to 1.5%. The upper Oligocene and middle-upper Miocene source rocks in the north Sakhalin basin are typical, and the organic carbon content ranges from 1 to 5%. The level of organic matter catagenesis and conversion into hydrocarbons is high because of the high differential geothermal gradient in the basins, 30-50[degrees]C per km. Porous sandstones in the Miocene form the reservoirs in all fields with the exception of Okruzhnoye, where the pay zone is a siliceous claystone. Growth-fault rollovers and anticlines form the main traps ranging in area from 5 to 300 km[sup 2], with amplitudes between 100 and 600 m. both stratigraphic and structural traps have been identified. Considerable volumes of reserves are associated with the Miocene deposits of north Sakhalin, which are characterized by an optimum combination of oil source rocks, focused migration paths, and thick sequences of reservoirs and cap rocks. Six large fields have been discovered in the past 15 yr. Oil and condensate reserves stand at over 300 million MT, and gas reserves are about 900 billion m[sup 3].

  4. Uplink Scheduling and Adjacent-Channel Coupling Loss Analysis for TD-LTE Deployment

    PubMed Central

    Yeo, Woon-Young; Moon, Sung Ho

    2014-01-01

    TD-LTE, one of the two duplexing modes in LTE, operates in unpaired spectrum and has the advantages of TDD-based technologies. It is expected that TD-LTE will be more rapidly deployed in near future and most of WiMax operators will upgrade their networks to TD-LTE gradually. Before completely upgrading to TD-LTE, WiMax may coexist with TD-LTE in an adjacent frequency band. In addition, multiple TD-LTE operators may deploy their networks in adjacent bands. When more than one TDD network operates in adjacent frequency bands, severe interference may happen due to adjacent channel interference (ACI) and unsynchronized operations. In this paper, coexistence issues between TD-LTE and other systems are analyzed and coexistence requirements are provided. This paper has three research objectives. First, frame synchronization between TD-LTE and WiMax is discussed by investigating possible combinations of TD-LTE and WiMax configurations. Second, an uplink scheduling algorithm is proposed to utilize a leakage pattern of ACI in synchronized operations. Third, minimum requirements for coexistence in unsynchronized operations are analyzed by introducing a concept of adjacent-channel coupling loss. From the analysis and simulation results, we can see that coexistence of TD-LTE with other TDD systems is feasible if the two networks are synchronized. For the unsynchronized case, some special cell-site engineering techniques may be required to reduce the ACI. PMID:24707214

  5. Uplink scheduling and adjacent-channel coupling loss analysis for TD-LTE deployment.

    PubMed

    Yeo, Woon-Young; Moon, Sung Ho; Kim, Jae-Hoon

    2014-01-01

    TD-LTE, one of the two duplexing modes in LTE, operates in unpaired spectrum and has the advantages of TDD-based technologies. It is expected that TD-LTE will be more rapidly deployed in near future and most of WiMax operators will upgrade their networks to TD-LTE gradually. Before completely upgrading to TD-LTE, WiMax may coexist with TD-LTE in an adjacent frequency band. In addition, multiple TD-LTE operators may deploy their networks in adjacent bands. When more than one TDD network operates in adjacent frequency bands, severe interference may happen due to adjacent channel interference (ACI) and unsynchronized operations. In this paper, coexistence issues between TD-LTE and other systems are analyzed and coexistence requirements are provided. This paper has three research objectives. First, frame synchronization between TD-LTE and WiMax is discussed by investigating possible combinations of TD-LTE and WiMax configurations. Second, an uplink scheduling algorithm is proposed to utilize a leakage pattern of ACI in synchronized operations. Third, minimum requirements for coexistence in unsynchronized operations are analyzed by introducing a concept of adjacent-channel coupling loss. From the analysis and simulation results, we can see that coexistence of TD-LTE with other TDD systems is feasible if the two networks are synchronized. For the unsynchronized case, some special cell-site engineering techniques may be required to reduce the ACI. PMID:24707214

  6. Adjacent Codons Act in Concert to Modulate Translation Efficiency in Yeast.

    PubMed

    Gamble, Caitlin E; Brule, Christina E; Dean, Kimberly M; Fields, Stanley; Grayhack, Elizabeth J

    2016-07-28

    Translation elongation efficiency is largely thought of as the sum of decoding efficiencies for individual codons. Here, we find that adjacent codon pairs modulate translation efficiency. Deploying an approach in Saccharomyces cerevisiae that scored the expression of over 35,000 GFP variants in which three adjacent codons were randomized, we have identified 17 pairs of adjacent codons associated with reduced expression. For many pairs, codon order is obligatory for inhibition, implying a more complex interaction than a simple additive effect. Inhibition mediated by adjacent codons occurs during translation itself as GFP expression is restored by increased tRNA levels or by non-native tRNAs with exact-matching anticodons. Inhibition operates in endogenous genes, based on analysis of ribosome profiling data. Our findings suggest translation efficiency is modulated by an interplay between tRNAs at adjacent sites in the ribosome and that this concerted effect needs to be considered in predicting the functional consequences of codon choice. PMID:27374328

  7. Independent assessment to continue improvement: Implementing statistical process control at the Hanford Site

    SciTech Connect

    Hu, T.A.; Lo, J.C.

    1994-11-01

    A Quality Assurance independent assessment has brought about continued improvement in the PUREX Plant surveillance program at the Department of Energy`s Hanford Site. After the independent assessment, Quality Assurance personnel were closely involved in improving the surveillance program, specifically regarding storage tank monitoring. The independent assessment activities included reviewing procedures, analyzing surveillance data, conducting personnel interviews, and communicating with management. Process improvement efforts included: (1) designing data collection methods; (2) gaining concurrence between engineering and management, (3) revising procedures; and (4) interfacing with shift surveillance crews. Through this process, Statistical Process Control (SPC) was successfully implemented and surveillance management was improved. The independent assessment identified several deficiencies within the surveillance system. These deficiencies can be grouped into two areas: (1) data recording and analysis and (2) handling off-normal conditions. By using several independent assessment techniques, Quality Assurance was able to point out program weakness to senior management and present suggestions for improvements. SPC charting, as implemented by Quality Assurance, is an excellent tool for diagnosing the process, improving communication between the team members, and providing a scientific database for management decisions. In addition, the surveillance procedure was substantially revised. The goals of this revision were to (1) strengthen the role of surveillance management, engineering and operators and (2) emphasize the importance of teamwork for each individual who performs a task. In this instance we believe that the value independent assessment adds to the system is the continuous improvement activities that follow the independent assessment. Excellence in teamwork between the independent assessment organization and the auditee is the key to continuing improvement.

  8. Control technologies for remediation of contaminated soil and waste deposits at Superfund lead-battery recycling sites

    SciTech Connect

    Royer, M.D.; Selvakumar, A.; Gaire, R.

    1992-01-01

    The paper primarily addresses remediation of contaminated soils and waste deposits at defunct lead-acid battery recycling sites (LBRS) via immobilization and separation processes. Metallic lead and lead compounds are generally the principal contaminants of concern in soils and waste deposits. Other metals (e.g., cadmium, copper, arsenic, antimony, and selenium) are often present at LBRS. The article is primarily based on experience gained from: (1) Superfund site investigation, removal, and remedial actions, and (2) development and demonstration of control technologies under the Superfund Innovative Technology Evaluation (SITE) Program. The primary remedial options for lead contaminated soils and waste deposits include: (1) no action, (2) off-site disposal, (3) containment, (4) immobilization, (5) separation with resource recovery, and (6) separation without resource recovery.

  9. Site-specific control of silica mineralization on DNA using a designed peptide.

    PubMed

    Ozaki, Makoto; Nagai, Kazuma; Nishiyama, Hiroto; Tsuruoka, Takaaki; Fujii, Satoshi; Endoh, Tamaki; Imai, Takahito; Tomizaki, Kin-Ya; Usui, Kenji

    2016-03-01

    We developed a site-specific method for precipitating inorganic compounds using organic compounds, DNA, and designed peptides with peptide nucleic acids (PNAs). Such a system for site-specific precipitation represents a powerful tool for use in nanobiochemistry and materials chemistry. PMID:26690695

  10. BIG SAGEBRUSH LEAF AREA DYNAMICS ON A BURNED, GRAZED AND CONTROL SITE IN THE SAGEBRUSH STEPPE

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Big sagebrush (Artemisia tridentata Nutt.) is an important shrub for wildlife habitat and carbon sequestration in the western U.S. The effects of fire and grazing on leaf area development of big sagebrush were investigated in plots established on a 16-ha burned site, a 12-ha grazed site and 16-ha c...

  11. Current Human Exposures Under Control at High-Priority Cleanup Sites

    EPA Science Inventory

    This indicator describes the numbers of National Priority List (NPL) Indicator Baseline (Superfund) sites and Resource Conservation and Recovery Act (RCRA) Cleanup Baseline sites from 2000 to 2007 for which government officials have determined that 1) humans are not e...

  12. Migration of Contaminated Ground Water Under Control at High-Priority Cleanup Sites

    EPA Science Inventory

    This indicator describes the percentage of National Priority List (NPL) or Superfund Indicator Baseline sites and Resource Conservation and Recovery Act (RCRA) Cleanup Baseline sites from 2000 to 2007 where government officials have determined that contaminated ground water is...

  13. On-the-go nitrogen sensing and fertilizer control for site-specific crop management

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In-field site-specific nitrogen (N) management increases crop yield, reduces N application to minimize the risk of nitrate contamination of ground water, and thus reduces farming cost. Real-time N sensing and fertilization is required for efficient N management. An ‘on-the-go’ site-specific N manage...

  14. Dynamics of re-constitution of the human nuclear proteome after cell division is regulated by NLS-adjacent phosphorylation

    PubMed Central

    Róna, Gergely; Borsos, Máté; Ellis, Jonathan J; Mehdi, Ahmed M; Christie, Mary; Környei, Zsuzsanna; Neubrandt, Máté; Tóth, Judit; Bozóky, Zoltán; Buday, László; Madarász, Emília; Bodén, Mikael; Kobe, Bostjan; Vértessy, Beáta G

    2014-01-01

    Phosphorylation by the cyclin-dependent kinase 1 (Cdk1) adjacent to nuclear localization signals (NLSs) is an important mechanism of regulation of nucleocytoplasmic transport. However, no systematic survey has yet been performed in human cells to analyze this regulatory process, and the corresponding cell-cycle dynamics have not yet been investigated. Here, we focused on the human proteome and found that numerous proteins, previously not identified in this context, are associated with Cdk1-dependent phosphorylation sites adjacent to their NLSs. Interestingly, these proteins are involved in key regulatory events of DNA repair, epigenetics, or RNA editing and splicing. This finding indicates that cell-cycle dependent events of genome editing and gene expression profiling may be controlled by nucleocytoplasmic trafficking. For in-depth investigations, we selected a number of these proteins and analyzed how point mutations, expected to modify the phosphorylation ability of the NLS segments, perturb nucleocytoplasmic localization. In each case, we found that mutations mimicking hyper-phosphorylation abolish nuclear import processes. To understand the mechanism underlying these phenomena, we performed a video microscopy-based kinetic analysis to obtain information on cell-cycle dynamics on a model protein, dUTPase. We show that the NLS-adjacent phosphorylation by Cdk1 of human dUTPase, an enzyme essential for genomic integrity, results in dynamic cell cycle-dependent distribution of the protein. Non-phosphorylatable mutants have drastically altered protein re-import characteristics into the nucleus during the G1 phase. Our results suggest a dynamic Cdk1-driven mechanism of regulation of the nuclear proteome composition during the cell cycle. PMID:25483092

  15. THE INTEGRATION OF ENGINEERED AND INSTITUTIONAL CONTROLS: A CASE STUDY APPROACH WITH LESSONS LEARNED FROM PREVIOUSLY CLOSED SITES

    SciTech Connect

    Kevin M. Kostelnik; James H. Clarke; Jerry L. Harbour

    2005-02-01

    Environmental remediation efforts that are underway at hundreds of contaminated sites in the United States will not be able to remediate large portions of those sites to conditions that would permit unrestricted access. Rather, large volumes of waste materials, contaminated soils and cleanup residuals will have to be isolated either in place or in new, often on-site, disposal cells with long term monitoring, maintenance and institutional control needs. The challenge continues to be to provide engineering systems and controls that can ensure the protection of public health and the environment over very long time horizons (hundreds to perhaps thousands of years) with minimal intervention. Effective long term management of legacy hazardous and nuclear waste requires an integrated approach that addresses both the engineered containment and control system itself and the institutional controls and other responsibilities that are needed. Decisions concerning system design, monitoring and maintenance, and the institutional controls that will be employed are best done through a "risk-nformed, performance-based" approach. Such an approach should incorporate an analysis of potential "failure" modes and consequences for all important system features, together with lessons learned from experience with systems already in place. The authors will present the preliminary results of a case study approach that included several sites where contamination isolation systems including institutional controls have been implemented. The results are being used together with failure trees and logic diagrams that have been developed for both the engineered barriers and the institutional controls. The use of these analytical tools to evaluate the potential for different levels of failure and associated consequences will be discussed. Of special interest is the robustness of different approaches to providing long-term protection through redundancy and defense in depth.

  16. Involvement of winged eye encoding a chromatin-associated bromo-adjacent homology domain protein in disc specification

    PubMed Central

    Katsuyama, Tomonori; Sugawara, Tomo; Tatsumi, Masanobu; Oshima, Yoshiteru; Gehring, Walter J.; Aigaki, Toshiro; Kurata, Shoichiro

    2005-01-01

    How organ identity is determined is a fundamental question in developmental biology. In Drosophila, field-specific selector genes, such as eyeless (ey) for eyes and vestigial (vg) for wings, participate in the determination of imaginal disc-specific identity. We performed gain-of-function screening and identified a gene named winged eye (wge), which encodes a bromo-adjacent homology domain protein that localizes at specific sites on chromosomes in a bromo-adjacent homology domain-dependent manner. Overexpression of wge-induced ectopic wings with antero-posterior and dorso-ventral axes in the eye field in a region-specific Hox gene-(Antennapedia) independent manner. Overexpression of wge was sufficient for ectopic expression of vg in eye discs. A context-dependent requirement of wge was demonstrated for vg expression in wing discs and for expression of eyes absent (eya), a control gene for eye development downstream of ey, in eye discs. In contrast to vg, however, overexpression of wge inhibited EY-mediated expression of eya. Consistent with colocalization on polytene chromosomes of WGE and Posterior sex combs (PSC), a Polycomb group gene product, we demonstrated an antagonistic genetic interaction between wge and Psc. These findings suggest that wge functions in the determination of disc-specific identity, downstream of Hox genes. PMID:16247005

  17. Quantification of lincomycin resistance genes associated with lincomycin residues in waters and soils adjacent to representative swine farms in China

    PubMed Central

    Li, Liang; Sun, Jian; Liu, Baotao; Zhao, Donghao; Ma, Jun; Deng, Hui; Li, Xue; Hu, Fengyang; Liao, Xiaoping; Liu, Yahong

    2013-01-01

    Lincomycin is commonly used on swine farms for growth promotion as well as disease treatment and control. Consequently, lincomycin may accumulate in the environment adjacent to the swine farms in many ways, thereby influencing antibiotic resistance in the environment. Levels of lincomycin-resistance genes and lincomycin residues in water and soil samples collected from multiple sites near wastewater discharge areas were investigated in this study. Sixteen lincomycin-resistance and 16S rRNA genes were detected using real-time PCR. Three genes, lnu(F), erm(A), and erm(B), were detected in all water and soil samples except control samples. Lincomycin residues were determined by rapid resolution liquid chromatography-tandem mass spectrometry, with concentrations detected as high as 9.29 ng/mL in water and 0.97 ng/g in soil. A gradual reduction in the levels of lincomycin-resistance genes and lincomycin residues in the waters and soils were detected from multiple sites along the path of wastewater discharging to the surrounding environment from the swine farms. Significant correlations were found between levels of lincomycin-resistance genes in paired water and soil samples (r = 0.885, p = 0.019), and between lincomycin-resistance genes and lincomycin residues (r = 0.975, p < 0.01). This study emphasized the potential risk of dissemination of lincomycin-resistance genes such as lnu(F), erm(A), and erm(B), associated with lincomycin residues in surrounding environments adjacent to swine farms. PMID:24348472

  18. Site-Competition Epitaxy for N-Type and P-Type Dopant Control in CVD Sic Epilayers

    NASA Technical Reports Server (NTRS)

    Larkin, D. J.

    1995-01-01

    The use of site-competition epitaxy, which is based on intentional variation of the Si/C ratio during epitaxy, has now been reproduced in numerous national and international laboratories. However, previous reports have only considered dopant incorporation control for epitaxy on the Si-face 6H-SiC(OOO1) substrates. Presented in this paper is the extension of this technique for control of phosphorous incorporation and also a comparison of controlled doping on C-face 6H-SiC(OOO1) versus Si-face 6H-SiC(OOO1) substrates for aluminum, boron, nitrogen, and phosphorous.

  19. 10 CFR 960.5-2-2 - Site ownership and control.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... NUCLEAR WASTE REPOSITORY Preclosure Guidelines Preclosure Radiological Safety § 960.5-2-2 Site ownership..., in accordance with the requirements of 10 CFR 60.121, ownership, surface and subsurface rights,...

  20. 10 CFR 960.5-2-2 - Site ownership and control.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... NUCLEAR WASTE REPOSITORY Preclosure Guidelines Preclosure Radiological Safety § 960.5-2-2 Site ownership..., in accordance with the requirements of 10 CFR 60.121, ownership, surface and subsurface rights,...

  1. Control of Ion Selectivity in LeuT: Two Na+ Binding Sites with two different mechanisms

    PubMed Central

    Noskov, Sergei Y.; Roux, Benoît

    2016-01-01

    The x-ray structure of LeuT, a bacterial homologue of Na+/Cl−-dependent neurotransmitter transporter, provides a great opportunity to better understand the molecular basis of monovalent cation selectivity in ion-coupled transporters. LeuT possesses two ion-binding sites, NA1 and NA2, which are highly selective for Na+. Extensive all-atom free energy molecular dynamics simulations of LeuT embedded in an explicit membrane are performed at different temperatures and various occupancy states of the binding sites to dissect the molecular mechanism of ion selectivity. The results show that the two binding sites display robust selectivity for Na+ over K+ or Li+, the competing ions of most similar radii. Of particular interest, the mechanism primarily responsible for selectivity for each of the two binding sites appears to be different. In site NA1, selectivity for Na+ over K+ arises predominantly from the strong electrostatic field arising from the negatively charged carboxylate group of the leucine substrate coordinating the ion directly. In site NA2, which comprises only neutral ligands, selectivity for Na+ is enforced by the local structural restraints arising from the hydrogen-bonding network and the covalent connectivity of the poly-peptide chain surrounding the ion according to a snug-fit mechanism. PMID:18280500

  2. Herbicide interchange between a stream and the adjacent alluvial aquifer

    USGS Publications Warehouse

    Wang, W.; Squillace, P.

    1994-01-01

    Herbicide interchange between a stream and the adjacent alluvial aquifer and quantification of herbicide bank storage during high streamflow were investigated at a research site on the Cedar River flood plain, 10 km southeast of Cedar Rapids, Iowa. During high streamflow in March 1990, alachlor, atrazine, and metolachlor were detected at concentrations above background in water from wells as distant as 20, 50, and 10 m from the river's edge, respectively. During high streamflow in May 1990, alachlor, atrazine, cyanazine, and metolachlor were detected at concentrations above background as distant as 20, 50, 10, and 20 m from the river's edge, respectively. Herbicide bank storage took place during high streamflow when hydraulic gradients were from the river to the alluvial aquifer and the laterally infiltrating river water contained herbicide concentrations larger than background concentrations in the aquifer. The herbicide bank storage can be quantified by multiplying herbicide concentration by the "effective area" that a well represented and an assumed porosity of 0.25. During March 1990, herbicide bank storage values were calculated to be 1.7,79, and 4.0 mg/m for alachlor, atrazine, and metolachlor, respectively. During May 1990, values were 7.1, 54, 11, and 19 mg/m for alachlor, atrazine, cyanazine, and metolachlor, respectively. ?? 1994 American Chemical Society.

  3. View of north side from exterior stairs of adjacent building, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of north side from exterior stairs of adjacent building, bottom cut off by fringed buildings, view facing south-southwest - U.S. Naval Base, Pearl Harbor, Industrial X-Ray Building, Off Sixth Street, adjacent to and south of Facility No. 11, Pearl City, Honolulu County, HI

  4. Learning Non-Adjacent Regularities at Age 0 ; 7

    ERIC Educational Resources Information Center

    Gervain, Judit; Werker, Janet F.

    2013-01-01

    One important mechanism suggested to underlie the acquisition of grammar is rule learning. Indeed, infants aged 0 ; 7 are able to learn rules based on simple identity relations (adjacent repetitions, ABB: "wo fe fe" and non-adjacent repetitions, ABA: "wo fe wo", respectively; Marcus et al., 1999). One unexplored issue is…

  5. Delayed Acquisition of Non-Adjacent Vocalic Distributional Regularities

    ERIC Educational Resources Information Center

    Gonzalez-Gomez, Nayeli; Nazzi, Thierry

    2016-01-01

    The ability to compute non-adjacent regularities is key in the acquisition of a new language. In the domain of phonology/phonotactics, sensitivity to non-adjacent regularities between consonants has been found to appear between 7 and 10 months. The present study focuses on the emergence of a posterior-anterior (PA) bias, a regularity involving two…

  6. Impact of wound edge protection devices on surgical site infection after laparotomy: multicentre randomised controlled trial (ROSSINI Trial)

    PubMed Central

    Calvert, Melanie; Bartlett, David C; Gheorghe, Adrian; Redman, Val; Dowswell, George; Hawkins, William; Mak, Tony; Youssef, Haney; Richardson, Caroline; Hornby, Steven; Magill, Laura; Haslop, Richard; Wilson, Sue; Morton, Dion

    2013-01-01

    Objective To determine the clinical effectiveness of wound edge protection devices in reducing surgical site infection after abdominal surgery. Design Multicentre observer blinded randomised controlled trial. Participants Patients undergoing laparotomy at 21 UK hospitals. Interventions Standard care or the use of a wound edge protection device during surgery. Main outcome measures Surgical site infection within 30 days of surgery, assessed by blinded clinicians at seven and 30 days and by patient’s self report for the intervening period. Secondary outcomes included quality of life, duration of stay in hospital, and the effect of characteristics of the patient and operation on the efficacy of the device. Results 760 patients were enrolled with 382 patients assigned to the device group and 378 to the control group. Six patients in the device group and five in the control group did not undergo laparotomy. Fourteen patients, seven in each group, were lost to follow-up. A total of 184 patients experienced surgical site infection within 30 days of surgery, 91/369 (24.7%) in the device group and 93/366 (25.4%) in the control group (odds ratio 0.97, 95% confidence interval 0.69 to 1.36; P=0.85). This lack of benefit was consistent across wound assessments performed by clinicians and those reported by patients and across all secondary outcomes. In the secondary analyses no subgroup could be identified in which there was evidence of clinical benefit associated with use of the device. Conclusions Wound edge protection devices do not reduce the rate of surgical site infection in patients undergoing laparotomy, and therefore their routine use for this role cannot be recommended. Trial registration Current Controlled Trials ISRCTN 40402832 PMID:23903454

  7. Precise temporal control of the eye regulatory gene Pax6 via enhancer-binding site affinity.

    PubMed

    Rowan, Sheldon; Siggers, Trevor; Lachke, Salil A; Yue, Yingzi; Bulyk, Martha L; Maas, Richard L

    2010-05-15

    How transcription factors interpret the cis-regulatory logic encoded within enhancers to mediate quantitative changes in spatiotemporally restricted expression patterns during animal development is not well understood. Pax6 is a dosage-sensitive gene essential for eye development. Here, we identify the Prep1 (pKnox1) transcription factor as a critical dose-dependent upstream regulator of Pax6 expression during lens formation. We show that Prep1 activates the Pax6 lens enhancer by binding to two phylogenetically conserved lower-affinity DNA-binding sites. Finally, we describe a mechanism whereby Pax6 levels are determined by transcriptional synergy of Prep1 bound to the two sites, while timing of enhancer activation is determined by binding site affinity. PMID:20413611

  8. Distribution of 125I-ferrotransferrin binding sites in the mesencephalon of control subjects and patients with Parkinson's disease.

    PubMed

    Faucheux, B A; Hirsch, E C; Villares, J; Selimi, F; Mouatt-Prigent, A; Javoy-Agid, F; Hauw, J J; Agid, Y

    1993-06-01

    Iron is abnormally accumulated in the substantia nigra pars compacta of patients with Parkinson's disease (PD). Because neuronal and glial iron uptake seems to be mediated by the binding of ferrotransferrin to a specific high-affinity receptor on the cell surface, the number of transferrin receptors could be altered in this disease. The regional distribution of specific binding sites for human 125I-diferric transferrin has been studied in the mesencephalon, on cryostat-cut sections from autopsy brains of control subjects and parkinsonian patients by in vitro autoradiography. Densities of binding sites were highest in the central gray substance (approximately 10 fmol/mg of tissue equivalent), intermediate in the catecholaminergic cell group A8, superior colliculus, and ventral tegmental area, and almost nonexistent in the substantia nigra. The density of 125I-transferrin binding sites was not significantly different between parkinsonian and control brains in any region analyzed. These results show that in the mesencephalon the regional density of transferrin binding sites is lowest in the dopaminergic cell groups, which are the most vulnerable to PD, and suggest that iron does not accumulate through an increased density of transferrin receptors at the level of the substantia nigra. PMID:8492137

  9. Environmental assessment for the offsite commercial cleaning of controlled and routine laundry from the Savannah River Site

    SciTech Connect

    Not Available

    1994-12-01

    This Environmental Assessment (EA) has been prepared by the U. S. Department of Energy (DOE) to assess the potential environmental impacts associated with the offsite commercial cleaning of controlled and routine laundry from the Savannah River Site (SRS) near Aiken, South Carolina. Controlled laundry consists of protective clothing and respirator equipment potentially containing radioactive contamination resulting from activities at SRS facilities. Routine laundry includes uncontaminated protective clothing. The aging onsite SRS laundry facility does not comply with current low hazard nuclear facility standards in DOE Order 6430.1. Constructing a new facility on site or upgrading the existing facility have prohibitive costs. The option to seek a commercial offsite vendor was selected as a viable alternative.

  10. Planting native species to control site reinfestation by Japanese knotweed (Polygonum cuspidatum)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Japanese knotweed (Polygonum cuspidatum) is an invasive species that has quickly become a serious problem both in riparian zones and in upland sites throughout the eastern US. It is an herbaceous perennial that can reach heights of 10 ft or more, and is capable of reproducing and quickly spreading b...

  11. Triple-site rTMS for the treatment of chronic tinnitus: a randomized controlled trial.

    PubMed

    Lehner, Astrid; Schecklmann, Martin; Greenlee, Mark W; Rupprecht, Rainer; Langguth, Berthold

    2016-01-01

    Recent research indicates that tinnitus is related to alterations of neural networks including temporal, parietal, and prefrontal brain regions. The current study examines a rTMS protocol which targets three central nodes of these networks in a two-arm randomized parallel group trial. Overall, 49 patients with chronic tinnitus were randomized to receive either triple-site stimulation (left dorsolateral prefrontal stimulation, 1000 pulses, 20 Hz plus left and right temporoparietal stimulation, 1000 pulses each, 1 Hz) or single-site stimulation (left temporoparietal stimulation, 3000 pulses, 1 Hz). Both groups were treated in ten sessions. Tinnitus severity as measured by the tinnitus questionnaire was assessed before rTMS (day1), after rTMS (day12) and at two follow-up visits (day 90 and day 180). The triple-site protocol was well tolerated. There was a significant reduction in tinnitus severity for both treatment groups. The triple-site group tended to show a more pronounced treatment effect at day 90. However, the measurement time point x group interaction effect was not significant. The current results confirm former studies that indicated a significant reduction of tinnitus severity after rTMS treatment. No significant superiority of the multisite protocol was observed. Future approaches for the enhancement of treatment effects are discussed. PMID:26927363

  12. CONTROL TECHNOLOGIES FOR REMEDIATION OF CONTAMINATED SOIL AND WASTE DEPOSITS AT SUPERFUND LEAD BATTERY RECYCLING SITES

    EPA Science Inventory

    This paper primarily addresses remediation of contaminated soils and waste deposits at defunct lead-acid battery recycling sites (LBRS) via immobilization and separation processes. A defunct LBRS is a facility at which battery breaking, secondary lead smelting, or both operations...

  13. Triple-site rTMS for the treatment of chronic tinnitus: a randomized controlled trial

    PubMed Central

    Lehner, Astrid; Schecklmann, Martin; Greenlee, Mark W.; Rupprecht, Rainer; Langguth, Berthold

    2016-01-01

    Recent research indicates that tinnitus is related to alterations of neural networks including temporal, parietal, and prefrontal brain regions. The current study examines a rTMS protocol which targets three central nodes of these networks in a two-arm randomized parallel group trial. Overall, 49 patients with chronic tinnitus were randomized to receive either triple-site stimulation (left dorsolateral prefrontal stimulation, 1000 pulses, 20 Hz plus left and right temporoparietal stimulation, 1000 pulses each, 1 Hz) or single-site stimulation (left temporoparietal stimulation, 3000 pulses, 1 Hz). Both groups were treated in ten sessions. Tinnitus severity as measured by the tinnitus questionnaire was assessed before rTMS (day1), after rTMS (day12) and at two follow-up visits (day 90 and day 180). The triple-site protocol was well tolerated. There was a significant reduction in tinnitus severity for both treatment groups. The triple-site group tended to show a more pronounced treatment effect at day 90. However, the measurement time point x group interaction effect was not significant. The current results confirm former studies that indicated a significant reduction of tinnitus severity after rTMS treatment. No significant superiority of the multisite protocol was observed. Future approaches for the enhancement of treatment effects are discussed. PMID:26927363

  14. The interactive on-site inspection system: An information management system to support arms control inspections

    SciTech Connect

    DeLand, S.M.; Widney, T.W.; Horak, K.E.; Caudell, R.B.; Grose, E.M.

    1996-12-01

    The increasing use of on-site inspection (OSI) to meet the nation`s obligations with recently signed treaties requires the nation to manage a variety of inspection requirements. This document describes a prototype automated system to assist in the preparation and management of these inspections.

  15. Integrated decision support, sensor networks and adaptive control for wireless site-specific sprinkler irrigation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The development of site-specific sprinkler irrigation water management systems will be a major factor in future efforts to improve the various efficiencies of water-use and to support a sustainable irrigated environment. The challenge is to develop fully integrated management systems with supporting...

  16. Integrated Decision Support, Sensor Networks and Adaptive Control for Wireless Site-specific Sprinkler Irrigation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The development of site-specific sprinkler irrigation water management systems will be a major factor in future efforts to improve the various efficiencies of water-use and to support a sustainable irrigated environment. The challenge is to develop fully integrated management systems with supporting...

  17. CONTROL TECHNOLOGIES FOR REMEDIATION OF CONTAMINATED SOIL AND WASTE DEPOSITS AT SUPERFUND LEAD BATTERY SITES

    EPA Science Inventory

    This paper primarily addresses remediation of contaminated soils and waste deposits at defunct lead-acid battery recycling sites (LBRS) via immobilization and separation processes. efunct LBRS is a facility at which battery breaking, secondary lead smelting, or both operations we...

  18. EVALUATION OF CONTROL CHART METHODOLOGIES FOR RCRA (RESOURCE CONSERVATION AND RECOVERY ACT) WASTE SITES

    EPA Science Inventory

    The report is a discussion of decision rules relating to the monitoring of ground water at hazardous waste sites that are subject to regulation under the Resource Conservation and Recovery Act of 1976 (RCRA). The final rule for RCRA regulations 40CFR part 264 was published Octobe...

  19. Explaining spatial patterns of sap flow: day-to-day shifts in relevance of site- and tree-specific controls

    NASA Astrophysics Data System (ADS)

    Hassler, Sibylle K.; Weiler, Markus; Blume, Theresa

    2015-04-01

    Transpiration is a key process in the hydrological cycle and a sound understanding and quantification of transpiration is essential for management decisions and for hydrological and climatological modelling. To assess transpiration at the tree scale sap flow velocity is commonly measured. Besides atmospheric conditions and soil moisture state, tree-specific characteristics such as species, size or social status control sap flow of individual trees. Within forest stands, properties such as species composition, basal area or stem number also affect sap flow via competition or facilitation mechanisms. Finally, sap flow patterns might also be influenced by landscape-scale characteristics such as geology, slope position or aspect because they affect water and energy availability; however, so far little is known about these larger-scale controls. We studied the relative importance of various tree- and site-specific characteristics with linear statistical models for daily sap velocity observations on 38 trees at 12 locations in mixed beech and oak forests in a catchment in Luxemburg. The temporal variation of the predictors' importance for sap velocity patterns was then related to hydro-meteorological conditions. Results indicate that a combination of tree- and site-specific controls influence sap velocity patterns, namely tree species, tree diameter, stand basal area, geology and aspect. The temporal dynamics of these controls are related to hydro-meteorological conditions, with tree-specific controls dominating when the atmospheric gradient is strong, i.e. the vapour pressure deficit is large, leading to higher sap velocities, whereas landscape-scale site characteristics are more important during weak atmospheric gradients. The importance of individual predictors also varies between spring and summer, probably due to different soil moisture and atmospheric conditions of the two periods. We conclude that both tree- and site-specific characteristics control sap velocity

  20. Controlling activation site density by low-energy far-field stimulation in cardiac tissue

    NASA Astrophysics Data System (ADS)

    Hörning, Marcel; Takagi, Seiji; Yoshikawa, Kenichi

    2012-06-01

    Tachycardia and fibrillation are potentially fatal arrhythmias associated with the formation of rotating spiral waves in the heart. Presently, the termination of these types of arrhythmia is achieved by use of antitachycardia pacing or cardioversion. However, these techniques have serious drawbacks, in that they either have limited application or produce undesirable side effects. Low-energy far-field stimulation has recently been proposed as a superior therapy. This proposed therapeutic method would exploit the phenomenon in which the application of low-energy far-field shocks induces a large number of activation sites (“virtual electrodes”) in tissue. It has been found that the formation of such sites can lead to the termination of undesired states in the heart and the restoration of normal beating. In this study we investigate a particular aspect of this method. Here we seek to determine how the activation site density depends on the applied electric field through in vitro experiments carried out on neonatal rat cardiac tissue cultures. The results indicate that the activation site density increases exponentially as a function of the intracellular conductivity and the level of cell isotropy. Additionally, we report numerical results obtained from bidomain simulations of the Beeler-Reuter model that are quantitatively consistent with our experimental results. Also, we derive an intuitive analytical framework that describes the activation site density and provides useful information for determining the ratio of longitudinal to transverse conductivity in a cardiac tissue culture. The results obtained here should be useful in the development of an actual therapeutic method based on low-energy far-field pacing. In addition, they provide a deeper understanding of the intrinsic properties of cardiac cells.

  1. Site environmental programs

    SciTech Connect

    Schmidt, J.W.; Hanf, R.W.

    1995-06-01

    This section of the 1994 Hanford Site Environmental Report summarizes the site environmental programs. Effluent monitoring and environmental surveillance programs monitor for impacts from operations in several areas. The first area consists of the point of possible release into the environment. The second area consists of possible contamination adjacent to DOE facilities, and the third area is the general environment both on and off the site.

  2. Which dressing do donor site wounds need?: study protocol for a randomized controlled trial

    PubMed Central

    2011-01-01

    Background Donor site wounds after split-skin grafting are rather 'standard' wounds. At present, lots of dressings and topical agents for donor site wounds are commercially available. This causes large variation in the local care of these wounds, while the optimum 'standard' dressing for local wound care is unclear. This protocol describes a trial in which we investigate the effectiveness of various treatment options for these donor site wounds. Methods A 14-center, six-armed randomized clinical trial is being carried out in the Netherlands. An a-priori power analysis and an anticipated dropout rate of 15% indicates that 50 patients per group are necessary, totaling 300 patients, to be able to detect a 25% quicker mean time to complete wound healing. Randomization has been computerized to ensure allocation concealment. Adult patients who need a split-skin grafting operation for any reason, leaving a donor site wound of at least 10 cm2 are included and receive one of the following dressings: hydrocolloid, alginate, film, hydrofiber, silicone dressing, or paraffin gauze. No combinations of products from other intervention groups in this trial are allowed. Optimum application and changes of these dressings are pursued according to the protocol as supplied by the dressing manufacturers. Primary outcomes are days to complete wound healing and pain (using a Visual Analogue Scale). Secondary outcomes are adverse effects, scarring, patient satisfaction, and costs. Outcome assessors unaware of the treatment allocation will assess whether or not an outcome has occurred. Results will be analyzed according to the intention to treat principle. The first patient was randomized October 1, 2009. Discussion This study will provide comprehensive data on the effectiveness of different treatment options for donor site wounds. The dressing(s) that will prevail in effectiveness, satisfaction and costs will be promoted among clinicians dealing with such patients. Thus, we aim to

  3. Effectiveness of urban stormwater control measures in a 100-acre test site

    EPA Science Inventory

    Kansas City, Missouri chose a test area (100 acres) and a control area (80 acres) to test the effectiveness of urban stormwater control (SCM) deployment in conjunction with the USEPA, University of Alabama, University of Missouri-Kansas City and TetraTech. Both the test and cont...

  4. 75 FR 77664 - Honeywell International, Inc., Automation and Control Solutions Division, Including On-Site...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-13

    ... of Honeywell International, Inc., Automation and Control Solutions Division, Rock Island, Illinois. The notice was published in the Federal Register on August 13, 2010 (75 FR 49531). At the request of a... Employment and Training Administration Honeywell International, Inc., Automation and Control...

  5. Mitochondrial DNA control region sequences in Koreans: identification of useful variable sites and phylogenetic analysis for mtDNA data quality control.

    PubMed

    Lee, Hwan Young; Yoo, Ji-Eun; Park, Myung Jin; Chung, Ukhee; Shin, Kyoung-Jin

    2006-01-01

    We have established a high-quality mtDNA control region sequence database for Koreans. To identify polymorphic sites and to determine their frequencies and haplotype frequencies, the complete mtDNA control region was sequenced in 593 Koreans, and major length variants of poly-cytosine tracts in HV2 and HV3 were determined in length heteroplasmic individuals by PCR analysis using fluorescence-labeled primers. Sequence comparison showed that 494 haplotypes defined by 285 variable sites were found when the major poly-cytosine tract genotypes were considered in distinguishing haplotypes, whereas 441 haplotypes were found when the poly-cytosine tracts were ignored. Statistical parameters indicated that analysis of partial mtDNA control region which encompasses the extended regions of HV1 and HV2, CA dinucleotide repeats in HV3 and nucleotide position 16497, 16519, 456, 489 and 499 (HV1ex+HV2ex+HV3CA+5SNPs) and the analysis of another partial mtDNA control region including extended regions of HV1 and HV2, HV3 region and nucleotide position 16497 and 16519 (HV1ex+HV2ex+HV3+2SNPs) can be used as efficient alternatives for the analysis of the entire mtDNA control region in Koreans. Also, we collated the basic informative SNPs, suggested the important mutation motifs for the assignment of East Asian haplogroups, and classified 592 Korean mtDNAs (99.8%) into various East Asian haplogroups or sub-haplogroups. Haplogroup-directed database comparisons confirmed the absence of any major systematic errors in our data, e.g., a mix-up of site designations, base shifts or mistypings. PMID:16177905

  6. Fugitive dust emission source profiles and assessment of selected control strategies for particulate matter at gravel processing sites in Taiwan.

    PubMed

    Chang, Chang-Tang; Chang, Yu-Min; Lin, Wen-Yinn; Wu, Ming-Ching

    2010-10-01

    Particles emitted from gravel processing sites are one contributor to worsening air quality in Taiwan. Major pollution sources at gravel processing sites include gravel and sand piles, unpaved roads, material crushers, and bare ground. This study analyzed fugitive dust emission characteristics at each pollution source using several types of particle samplers, including total suspended particulates (TSP), suspended particulate (PM10), fine suspended particulate (PM2.5), particulate sizer, and dust-fall collectors. Furthermore, silt content and moisture in the gravel were measured to develop particulate emission factors. The results showed that TSP (< 100 microm) concentrations at the boundary of gravel sites ranged from 280 to 1290 microg/m3, which clearly exceeds the Taiwan hourly air quality standard of 500 microg/m3. Moreover, PM10 concentrations, ranging from 135 to 550 microg/m3, were also above the daily air quality standard of 125 microg/m3 and approximately 1.2 and 1.5 times the PM2.5 concentrations, ranging from 105 to 470 microg/m3. The size distribution analysis reveals that mass mean diameter and geometric standard deviation ranged from 3.2 to 5.7 microm and from 2.82 to 5.51, respectively. In this study, spraying surfactant was the most effective control strategy to abate windblown dust from unpaved roads, having a control efficiency of approximately 93%, which is significantly higher than using paved road strategies with a control efficiency of approximately 45%. For paved roads, wet suppression provided the best dust control efficiencies ranging from 50 to 83%. Re-vegetation of disturbed ground had dust control efficiencies ranging from 48 to 64%. PMID:21090554

  7. Thermoelastic response of thin metal films and their adjacent materials

    SciTech Connect

    Kang, S.; Yoon, Y.; Kim, J.; Kim, W.

    2013-01-14

    A pulsed laser beam applied to a thin metal film is capable of launching an acoustic wave due to thermal expansion. Heat transfer from the thin metal film to adjacent materials can also induce thermal expansion; thus, the properties of these adjacent materials (as well as the thin metal film) should be considered for a complete description of the thermoelastic response. Here, we show that adjacent materials with a small specific heat and large thermal expansion coefficient can generate an enhanced acoustic wave and we demonstrate a three-fold increase in the peak pressure of the generated acoustic wave on substitution of parylene for polydimethylsiloxane.

  8. Nitrous oxide emission from cropland and adjacent riparian buffers in contrasting hydrogeomorphic settings.

    PubMed

    Fisher, K; Jacinthe, P A; Vidon, P; Liu, X; Baker, M E

    2014-01-01

    Riparian buffers are important nitrate (NO) sinks in agricultural watersheds, but limited information is available regarding the intensity and control of nitrous oxide (NO) emission from these buffers. This study monitored (December 2009-May 2011) NO fluxes at two agricultural riparian buffers in the White River watershed in Indiana to assess the impact of land use and hydrogeomorphologic (HGM) attributes on emission. The study sites included a riparian forest in a glacial outwash/alluvium setting (White River [WR]) and a grassed riparian buffer in tile-drained till plains (Leary Weber Ditch [LWD]). Adjacent corn ( L.) fields were monitored for land use assessment. Analysis of variance identified season, land use (riparian buffer vs. crop field), and site geomorphology as major drivers of NO fluxes. Strong relationships between N mineralization and NO fluxes were found at both sites, but relationships with other nutrient cycling indicators (C/N ratio, dissolved organic C, microbial biomass C) were detected only at LWD. Nitrous oxide emission showed strong seasonal variability; the largest NO peaks occurred in late spring/early summer as a result of flooding at the WR riparian buffer (up to 27.8 mg NO-N m d) and N fertilizer application to crop fields. Annual NO emission (kg NO-N ha) was higher in the crop fields (WR: 7.82; LWD: 6.37) than in the riparian areas. A significant difference ( < 0.02) in annual NO emission between the riparian buffers was detected (4.32 vs. 1.03 kg NO-N ha at WR and LWD, respectively), and this difference was attributed to site geomorphology and flooding (WR is flood prone; no flooding occurred at tile-drained LWD). The study results demonstrate the significance of landscape geomorphology and land-stream connection (i.e., flood potential) as drivers of NO emission in riparian buffers and therefore argue that an HGM-based approach should be especially suitable for determination of regional NO budget in riparian ecosystems. PMID:25602568

  9. Hydrological Flowpaths and Their Controls at LBA Biogeochemistry Study Sites - Communalities, Contrasts and Representativeness

    NASA Astrophysics Data System (ADS)

    Elsenbeer, H.; Johnson, M.; Neill, C.

    2006-12-01

    Several LBA projects have focused on nutrient fluxes within and nutrient export from forested terra firme headwater catchments. Their physiographic settings encompass the most common soil types of Amazonia, i.e., Oxisols and Ultisols, and share the topography typical of recently dissected landscapes. We will explore to which degree pedological and geomorphological similarities among sites in Amazonas, Mato Grosso and Rondonia extend to near-surface hydrological behavior. We will then interpret differences in nutrient dynamics in terms of contrasting hydrological flowpaths and soil chemistry. Special attention will be given to the usefulness, or lack thereof, of soil taxonomic information as a predictor of near-surface hydrological and hydrochemical behavior in Amazonia, and to the role of riparian zones in masking terra firme processes. Against this background, we will evaluate the representativeness of these LBA sites in the Amazonian context.

  10. The Chromatin Remodelling Enzymes SNF2H and SNF2L Position Nucleosomes adjacent to CTCF and Other Transcription Factors

    PubMed Central

    Wiechens, Nicola; Gkikopoulos, Triantaffyllos; Schofield, Pieta; Rocha, Sonia; Owen-Hughes, Tom

    2016-01-01

    Within the genomes of metazoans, nucleosomes are highly organised adjacent to the binding sites for a subset of transcription factors. Here we have sought to investigate which chromatin remodelling enzymes are responsible for this. We find that the ATP-dependent chromatin remodelling enzyme SNF2H plays a major role organising arrays of nucleosomes adjacent to the binding sites for the architectural transcription factor CTCF sites and acts to promote CTCF binding. At many other factor binding sites SNF2H and the related enzyme SNF2L contribute to nucleosome organisation. The action of SNF2H at CTCF sites is functionally important as depletion of CTCF or SNF2H affects transcription of a common group of genes. This suggests that chromatin remodelling ATPase’s most closely related to the Drosophila ISWI protein contribute to the function of many human gene regulatory elements. PMID:27019336

  11. Using fuzzy logic analysis for siting decisions of infiltration trenches for highway runoff control.

    PubMed

    Ki, Seo Jin; Ray, Chittaranjan

    2014-09-15

    Determining optimal locations for best management practices (BMPs), including their field considerations and limitations, plays an important role for effective stormwater management. However, these issues have been often overlooked in modeling studies that focused on downstream water quality benefits. This study illustrates the methodology of locating infiltration trenches at suitable locations from spatial overlay analyses which combine multiple layers that address different aspects of field application into a composite map. Using seven thematic layers for each analysis, fuzzy logic was employed to develop a site suitability map for infiltration trenches, whereas the DRASTIC method was used to produce a groundwater vulnerability map on the island of Oahu, Hawaii, USA. In addition, the analytic hierarchy process (AHP), one of the most popular overlay analyses, was used for comparison to fuzzy logic. The results showed that the AHP and fuzzy logic methods developed significantly different index maps in terms of best locations and suitability scores. Specifically, the AHP method provided a maximum level of site suitability due to its inherent aggregation approach of all input layers in a linear equation. The most eligible areas in locating infiltration trenches were determined from the superposition of the site suitability and groundwater vulnerability maps using the fuzzy AND operator. The resulting map successfully balanced qualification criteria for a low risk of groundwater contamination and the best BMP site selection. The results of the sensitivity analysis showed that the suitability scores were strongly affected by the algorithms embedded in fuzzy logic; therefore, caution is recommended with their use in overlay analysis. Accordingly, this study demonstrates that the fuzzy logic analysis can not only be used to improve spatial decision quality along with other overlay approaches, but also is combined with general water quality models for initial and refined

  12. Site-selective nucleation and controlled growth of gold nanostructures in tobacco mosaic virus nanotubulars.

    PubMed

    Zhou, Kun; Zhang, Jianting; Wang, Qiangbin

    2015-06-01

    Site-selective biomineralization of Au nanostructures in the interior channel of Tobacco Mosaic Virus (TMV) is achieved by mutating threonine 103 in TMV to cysteine (T103C-TMV) to introduce the strong coordination interaction between the arrayed sulfhydryl ligands and gold species. By finely tuning the reaction conditions, Au nanoparticle chains and Au nanorods are successfully and exclusively synthesized inside the T103C-TMV nanotubes. PMID:25612918

  13. Hydrocarbon provinces and productive trends in Libya and adjacent areas

    SciTech Connect

    Missallati, A.A. Ltd., Tripoli )

    1988-08-01

    According to the age of major reservoirs, hydrocarbon occurrences in Libya and adjacent areas can be grouped into six major systems which, according to their geographic locations, can be classified into two major hydrocarbon provinces: (1) Sirte-Pelagian basins province, with major reservoirs ranging from middle-late Mesozoic to early Tertiary, and (2) Murzog-Ghadames basins province, with major reservoirs ranging from early Paleozoic to early Mesozoic. In the Sirte-Pelagian basins province, hydrocarbons have been trapped in structural highs or in stratigraphic wedge-out against structural highs and in carbonate buildups. Here, hydrocarbon generation is characterized by the combined effect of abundant structural relief and reservoir development in the same hydrocarbon systems of the same age, providing an excellent example of hydrocarbon traps in sedimentary basins that have undergone extensive tensional fracturing in a shallow marine environment. In the Murzog-Ghadames basins province, hydrocarbons have been trapped mainly in structural highs controlled by paleostructural trends as basement arches which acted as focal points for oil migration and accumulation.

  14. Site-1 protease-activated formation of lysosomal targeting motifs is independent of the lipogenic transcription control[S

    PubMed Central

    Klünder, Sarah; Heeren, Jörg; Markmann, Sandra; Santer, René; Braulke, Thomas; Pohl, Sandra

    2015-01-01

    Site-1 protease (S1P) cleaves membrane-bound lipogenic sterol regulatory element-binding proteins (SREBPs) and the α/β-subunit precursor protein of the N-acetylglucosamine-1-phosphotransferase forming mannose 6-phosphate (M6P) targeting markers on lysosomal enzymes. The translocation of SREBPs from the endoplasmic reticulum (ER) to the Golgi-resident S1P depends on the intracellular sterol content, but it is unknown whether the ER exit of the α/β-subunit precursor is regulated. Here, we investigated the effect of cholesterol depletion (atorvastatin treatment) and elevation (LDL overload) on ER-Golgi transport, S1P-mediated cleavage of the α/β-subunit precursor, and the subsequent targeting of lysosomal enzymes along the biosynthetic and endocytic pathway to lysosomes. The data showed that the proteolytic cleavage of the α/β-subunit precursor into mature and enzymatically active subunits does not depend on the cholesterol content. In either treatment, lysosomal enzymes are normally decorated with M6P residues, allowing the proper sorting to lysosomes. In addition, we found that, in fibroblasts of mucolipidosis type II mice and Niemann-Pick type C patients characterized by aberrant cholesterol accumulation, the proteolytic cleavage of the α/β-subunit precursor was not impaired. We conclude that S1P substrate-dependent regulatory mechanisms for lipid synthesis and biogenesis of lysosomes are different. PMID:26108224

  15. Characteristics and recent trends of sulfur dioxide at urban, rural, and background sites in north China: effectiveness of control measures.

    PubMed

    Lin, Weili; Xu, Xiaobin; Ma, Zhiqiang; Zhao, Huarong; Liu, Xiwen; Wang, Ying

    2012-01-01

    SO2 measurements made in recent years at sites in Beijing and its surrounding areas are performed to study the variations and trends of surface SO2 at different types of sites in Northern China. The overall average concentrations of SO2 are (16.8 +/- 13.1) ppb, (14.8 +/- 9.4) ppb, and (7.5 +/- 4.0) ppb at China Meteorological Administration (CMA, Beijing urban area), Gucheng (GCH, relatively polluted rural area, 110 km to the southwest of Beijing urban area), and Shangdianzi (SDZ, clean background area, 100 km to the northeast of Beijing urban area), respectively. The SO2 levels in winter (heating season) are 4-6 folds higher than those in summer. There are highly significant correlations among the daily means of SO2 at different sites, indicating regional characteristics of SO2 pollution. Diurnal patterns of surface SO2 at all sites have a common feature with a daytime peak, which is probably caused by the downward mixing and/or the advection transport of SO2-richer air over the North China Plain. The concentrations of SO2 at CMA and GCH show highly significant downward trends (-4.4 ppb/yr for CMA and -2.4 ppb/yr for GCH), while a less significant trend (-0.3 ppb/yr) is identified in the data from SDZ, reflecting the character of SDZ as a regional atmospheric background site in North China. The SO2 concentrations of all three sites show a significant decrease from period before to after the control measures for the 2008 Olympic Games, suggesting that the SO2 pollution control has long-term effectiveness and benefits. In the post-Olympics period, the mean concentrations of SO2 at CMA, GCH, and SDZ are (14.3 +/- 11.0) ppb, (12.1 +/- 7.7) ppb, and (7.5 +/- 4.0) ppb, respectively, with reductions of 26%, 36%, and 13%, respectively, compared to the levels before. Detailed analysis shows that the differences of temperature, relative humidity, wind speed, and wind direction were not the dominant factors for the significant differences of SO2 between the pre-Olympics and

  16. 73. PASSAGE ADJACENT TO ROOM 232, EAST WING, SECOND FLOOR, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    73. PASSAGE ADJACENT TO ROOM 232, EAST WING, SECOND FLOOR, LOOKING WEST BY NORTHWEST, SHOWING EASTERNMOST ARCH OF FORMER GREAT HALL NORTH ARCADE - Smithsonian Institution Building, 1000 Jefferson Drive, between Ninth & Twelfth Streets, Southwest, Washington, District of Columbia, DC

  17. View of viaduct, looking SE from roof of adjacent parking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of viaduct, looking SE from roof of adjacent parking garage. - Mulberry Street Viaduct, Spanning Paxton Creek & Cameron Street (State Route 230) at Mulberry Street (State Route 3012), Harrisburg, Dauphin County, PA

  18. Cement Leakage into Adjacent Vertebral Body Following Percutaneous Vertebroplasty

    PubMed Central

    Park, Jae Hoo; Kim, Hyeun Sung

    2016-01-01

    Percutaneous vertebroplasty (PV) is a minimally invasive procedure for osteoporotic vertebral compression fractures that fail to respond to conventional conservative treatment. It significantly improves intolerable back pain within hours, and has a low complication rate. Although rare, PV is not free of complications, most of which are directly related to cement leakage. Because of its association with new adjacent fracture, the importance of cement leakage into the adjacent disc space is paramount. Here, we report an interesting case of cement leakage into the adjacent upper vertebral body as well as disc space following PV. To the best of our knowledge, there has been no report of cement leakage into the adjacent vertebral body following PV. This rare case is presented along with a review of the literature. PMID:27437018

  19. 1. HEBRONVILLE MILL COMPLEX ADJACENT TO NORTHEAST CORRIDOR. HEBRONVILLE, BRISTOL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. HEBRONVILLE MILL COMPLEX ADJACENT TO NORTHEAST CORRIDOR. HEBRONVILLE, BRISTOL CO., MA. Sec. 4116, MP 193.75. - Northeast Railroad Corridor, Amtrak Route between RI/MA State Line & South Station, Boston, Suffolk County, MA

  20. 3. DODGEVILLE MILL COMPLEX ADJACENT TO NORTHEAST CORRIDOR DODGEVILLE, BRISTOL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. DODGEVILLE MILL COMPLEX ADJACENT TO NORTHEAST CORRIDOR DODGEVILLE, BRISTOL CO., MA. Sec. 4116, MP 195.55. - Northeast Railroad Corridor, Amtrak Route between RI/MA State Line & South Station, Boston, Suffolk County, MA

  1. Lock 4 View east of lock wall and adjacent ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Lock 4 - View east of lock wall and adjacent roadway built atop tow path. The gate pocket can be seen at center. - Savannah & Ogeechee Barge Canal, Between Ogeechee & Savannah Rivers, Savannah, Chatham County, GA

  2. 1. Ninth Street (west) facade. Adjacent on the north is ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Ninth Street (west) facade. Adjacent on the north is the 9th Street facade of 816 E Street. Both buildings were originally one property. - Riley Building, Rendezvous Adult Magazines & Films, 437 Ninth Street, Northwest, Washington, District of Columbia, DC

  3. 2. THREEQUARTER VIEW FROM ADJACENT ACCESS ROAD SHOWING THREE SPANS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. THREE-QUARTER VIEW FROM ADJACENT ACCESS ROAD SHOWING THREE SPANS AND NORTHWEST APPROACH SPANS, LOOKING SOUTHEAST - Red River Bridge, Spanning Red River at U.S. Highway 82, Garland, Miller County, AR

  4. 1. VIEW FROM ROOFTOP OF BUILDING (MOTEL) ADJACENT TO TECHWOOD ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. VIEW FROM ROOFTOP OF BUILDING (MOTEL) ADJACENT TO TECHWOOD HOMES, LOOKING SOUTH. GARAGE TO EXTREME LEFT, BUILDING 1 TO EXTREME RIGHT. - Techwood Homes (Public Housing), Bounded by North Avenue, Parker Street, William Street & Lovejoy Street, Atlanta, Fulton County, GA

  5. 3. View of north side of house facing from adjacent ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. View of north side of house facing from adjacent vacant property. Original wood lap siding and trim is covered by aluminum siding. Recessed side porch is in middle. - 645 South Eighteenth Street (House), Louisville, Jefferson County, KY

  6. 1. A BRICK AND CONCRETE FAN HOUSING ADJACENT TO ONE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. A BRICK AND CONCRETE FAN HOUSING ADJACENT TO ONE OF THE ADIT OPENINGS (VIEW TO THE NORTH). - Foster Gulch Mine, Fan Housing, Bear Creek 1 mile Southwest of Town of Bear Creek, Red Lodge, Carbon County, MT

  7. 7. August, 1970 9 ORANGE STREET, ADJACENT TO UNITARIAN CHURCH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. August, 1970 9 ORANGE STREET, ADJACENT TO UNITARIAN CHURCH (NOT IN STUDY AREA) - Orange & Union Streets Neighborhood Study, 8-31 Orange Street, 9-21 Union Street & Stone Alley, Nantucket, Nantucket County, MA

  8. OBLIQUE OF SOUTHWEST END AND SOUTHEAST SIDE, WITH ADJACENT FACILITY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    OBLIQUE OF SOUTHWEST END AND SOUTHEAST SIDE, WITH ADJACENT FACILITY 391 IN THE FOREGROUND. - U.S. Naval Base, Pearl Harbor, Joint Intelligence Center, Makalapa Drive in Makalapa Administration Area, Pearl City, Honolulu County, HI

  9. Complications in exodontia--accidental dislodgment to adjacent anatomical areas.

    PubMed

    Grandini, S A; Barros, V M; Salata, L A; Rosa, A L; Soares, U N

    1993-01-01

    The authors report 4 cases of accidental dislodgement of teeth to adjacent anatomical areas during extraction. The causes and their prevention are discussed and solutions for the problem are suggested. PMID:8241759

  10. 6. Detail, vertical guides adjacent to east portal of Tunnel ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Detail, vertical guides adjacent to east portal of Tunnel 28, view to southwest, 135mm lens with electronic flash fill. - Central Pacific Transcontinental Railroad, Tunnel No. 28, Milepost 134.75, Applegate, Placer County, CA

  11. VIEW OF CONSTRUCTION CAMP ROCK FEATURE WITH OVER, ADJACENT TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF CONSTRUCTION CAMP ROCK FEATURE WITH OVER, ADJACENT TO THE COLUMBIA SOUTHERN CANAL. LOOKING NORTHWEST - Tumalo Irrigation District, Tumalo Project, West of Deschutes River, Tumalo, Deschutes County, OR

  12. Pump house adjacent to the superintendent's house at the west ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Pump house adjacent to the superintendent's house at the west end of the complex near Highway 101. Detail of Holloshaft pump. View to the south. - Prairie Creek Fish Hatchery, Hwy. 101, Orick, Humboldt County, CA

  13. VIEW OF NORTHERN AND EASTERN SIDES FROM PARKING LOT ADJACENT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF NORTHERN AND EASTERN SIDES FROM PARKING LOT ADJACENT TO BUILDING 199 (POLICE STATION) - U.S. Naval Base, Pearl Harbor, Post Office, Avenue A near Eleventh Avenue, Pearl City, Honolulu County, HI

  14. 24. INTERIOR VIEW, WILLIAM GRAY AT SIZING GUAGE ADJACENT TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    24. INTERIOR VIEW, WILLIAM GRAY AT SIZING GUAGE ADJACENT TO BRADLEY HAMMER; NOTE THIS IS THE SAME TOOL AS BEING FORGED ABOVE - Warwood Tool Company, Foot of Nineteenth Street, Wheeling, Ohio County, WV

  15. Detail exterior view looking north showing piping system adjacent to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail exterior view looking north showing piping system adjacent to engine house. Gas cooling system is on far right. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  16. VIEW OF LAMP FIXTURE (EXTERIOR) ADJACENT TO ENTRANCE AT SOUTHWEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF LAMP FIXTURE (EXTERIOR) ADJACENT TO ENTRANCE AT SOUTHWEST CORNER OF BUILDING 23, FACING NORTH - Roosevelt Base, Auditorium-Gymnasium, West Virginia Street between Richardson & Reeves Avenues, Long Beach, Los Angeles County, CA

  17. 14. Charles Acey Cobb standing adjacent to the fish screen ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Charles Acey Cobb standing adjacent to the fish screen he designed and installed in the Congdon Canal, facing southeast. Photo dates ca. late 1920's. - Congdon Canal, Fish Screen, Naches River, Yakima, Yakima County, WA

  18. 52. EASTSIDE PLANT: GENERAL VIEW OF GOVERNOR ADJACENT TO GENERATOR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    52. EASTSIDE PLANT: GENERAL VIEW OF GOVERNOR ADJACENT TO GENERATOR - American Falls Water, Power & Light Company, Island Power Plant, Snake River, below American Falls Dam, American Falls, Power County, ID

  19. Interior building details of Building A, dungeon cell adjacent to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior building details of Building A, dungeon cell adjacent to northwest cell: granite and brick threshold, poured concrete floors, plastered finished walls, vaulted veiling; northwesterly view - San Quentin State Prison, Building 22, Point San Quentin, San Quentin, Marin County, CA

  20. VIEW OF CONCRETE CHANNEL ADJACENT TO TUMALO FEED CANAL INTAKE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF CONCRETE CHANNEL ADJACENT TO TUMALO FEED CANAL INTAKE STRUCTURE (DOWNSTREAM SIDE). LOOKING EAST/NORTHEAST - Tumalo Irrigation District, Tumalo Project, West of Deschutes River, Tumalo, Deschutes County, OR

  1. Site-specific growth and density control of carbon nanotubes by direct deposition of catalytic nanoparticles generated by spark discharge

    PubMed Central

    2013-01-01

    Catalytic iron nanoparticles generated by spark discharge were used to site-selectively grow carbon nanotubes (CNTs) and control their density. The generated aerosol nanoparticles were deposited on a cooled substrate by thermophoresis. The shadow mask on top of the cooled substrate enabled patterning of the catalytic nanoparticles and, thereby, patterning of CNTs synthesized by chemical vapor deposition. The density of CNTs could be controlled by varying the catalytic nanoparticle deposition time. It was also demonstrated that the density could be adjusted by changing the gap between the shadow mask and the substrate, taking advantage of the blurring effect of the deposited nanoparticles, for an identical deposition time. As all the processing steps for the patterned growth and density control of CNTs can be performed under dry conditions, we also demonstrated the integration of CNTs on fully processed, movable silicon microelectromechanical system (MEMS) structures. PMID:24090218

  2. Adjacent Segment Disease Perspective and Review of the Literature

    PubMed Central

    Saavedra-Pozo, Fanor M.; Deusdara, Renato A. M.; Benzel, Edward C.

    2014-01-01

    Background Adjacent segment disease has become a common topic in spine surgery circles because of the significant increase in fusion surgery in recent years and the development of motion preservation technologies that theoretically should lead to a decrease in this pathology. The purpose of this review is to organize the evidence available in the current literature on this subject. Methods For this literature review, a search was conducted in PubMed with the following keywords: adjacent segment degeneration and disease. Selection, review, and analysis of the literature were completed according to level of evidence. Results The PubMed search identified 850 articles, from which 41 articles were selected and reviewed. The incidence of adjacent segment disease in the cervical spine is close to 3% without a significant statistical difference between surgical techniques (fusion vs arthroplasty). Authors report the incidence of adjacent segment disease in the lumbar spine to range from 2% to 14%. Damage to the posterior ligamentous complex and sagittal imbalances are important risk factors for both degeneration and disease. Conclusion Insufficient evidence exists at this point to support the idea that total disc arthroplasty is superior to fusion procedures in minimizing the incidence of adjacent segment disease. The etiology is most likely multifactorial but it is becoming abundantly clear that adjacent segment disease is not caused by motion segment fusion alone. Fusion plus the presence of abnormal end-fusion alignment appears to be a major factor in creating end-fusion stresses that result in adjacent segment degeneration and subsequent disease. The data presented cast further doubt on previously established rationales for total disc arthroplasty, at least with regard to the effect of total disc arthroplasty on adjacent segment degeneration pathology. PMID:24688337

  3. Approximating the largest eigenvalue of network adjacency matrices

    NASA Astrophysics Data System (ADS)

    Restrepo, Juan G.; Ott, Edward; Hunt, Brian R.

    2007-11-01

    The largest eigenvalue of the adjacency matrix of a network plays an important role in several network processes (e.g., synchronization of oscillators, percolation on directed networks, and linear stability of equilibria of network coupled systems). In this paper we develop approximations to the largest eigenvalue of adjacency matrices and discuss the relationships between these approximations. Numerical experiments on simulated networks are used to test our results.

  4. Investigation of processes controlling summertime gaseous elemental mercury oxidation at midlatitudinal marine, coastal, and inland sites

    NASA Astrophysics Data System (ADS)

    Ye, Zhuyun; Mao, Huiting; Lin, Che-Jen; Kim, Su Youn

    2016-07-01

    A box model incorporating a state-of-the-art chemical mechanism for atmospheric mercury (Hg) cycling was developed to investigate the oxidation of gaseous elemental mercury (GEM) at three locations in the northeastern United States: Appledore Island (AI; marine), Thompson Farm (TF; coastal, rural), and Pack Monadnock (PM; inland, rural, elevated). The chemical mechanism in this box model included the most up-to-date Hg and halogen chemistry. As a result, the box model was able to simulate reasonably the observed diurnal cycles of gaseous oxidized mercury (GOM) and chemical speciation bearing distinct differences between the three sites. In agreement with observations, simulated GOM diurnal cycles at AI and TF showed significant daytime peaks in the afternoon and nighttime minimums compared to flat GOM diurnal cycles at PM. Moreover, significant differences in the magnitude of GOM diurnal amplitude (AI > TF > PM) were captured in modeled results. At the coastal and inland sites, GEM oxidation was predominated by O3 and OH, contributing 80-99 % of total GOM production during daytime. H2O2-initiated GEM oxidation was significant (˜ 33 % of the total GOM) at the inland site during nighttime. In the marine boundary layer (MBL) atmosphere, Br and BrO became dominant GEM oxidants, with mixing ratios reaching 0.1 and 1 pptv, respectively, and contributing ˜ 70 % of the total GOM production during midday, while O3 dominated GEM oxidation (50-90 % of GOM production) over the remaining day when Br and BrO mixing ratios were diminished. The majority of HgBr produced from GEM+Br was oxidized by NO2 and HO2 to form brominated GOM species. Relative humidity and products of the CH3O2+BrO reaction possibly significantly affected the mixing ratios of Br or BrO radicals and subsequently GOM formation. Gas-particle partitioning could potentially be important in the production of GOM as well as Br and BrO at the marine site.

  5. Hydrologic characteristics of low-impact stormwater control measures at two sites in northeastern Ohio, 2008-13

    USGS Publications Warehouse

    Darner, Robert A.; Shuster, William D.; Dumouchelle, Denise H.

    2015-01-01

    This report updates and examines hydrologic data gathered to characterize the performance of two stormwater-control measure (SCM) sites in the Chagrin River watershed, Ohio. At the Sterncrest Drive site, roadside bioswales and rain gardens were used to alleviate drainage problems in this residential neighborhood area. At the Washington Street site, a treatment train (including a pervious-paver system, rain garden, and bioswales) was used to reduce and delay stormwater runoff at a small business development. Selected metrics were used to demonstrate SCM system performance with regard to stormwater-management objectives at each site. Rain-garden overflow-frequency data collected at the Sterncrest Drive site during 2008–13 were used to characterize system sensitivity to rainfall characteristics. Approximately 70 percent of storms exceeding 0.75 inches during 3 hours or more resulted in overflows. Drainage-design features that may restrict flow through the system were identified. Overall, the data and local observations confirmed the continued success of the SCM at the Sterncrest Drive site in preventing roadway closure due to flooding. The additional years of data collected at the Washington Street site indicated that a previous analysis of increased runoff removal, based on only the first 2 years (2009–10) of data, provided premature conclusions. With 5 years of data (2009–13) and adjusting for changes in rainfall characteristics, it appears that the percentage of runoff removed by the system is decreasing; however, the lag time (time from onset of rainfall to runoff) has remained nearly constant. The annual mean percent removal for 2010–13 ranged from 55 to 37 percent with an overall mean of 45 percent, and this does meet the project objective of reducing runoff from the business complex. One possible explanation for the combination of increased volume of runoff and no change in the timing of runoff is the preferential flow paths developed in the SCM

  6. Growth and decline of shoreline industry in Sydney estuary (Australia) and influence on adjacent estuarine sediments.

    PubMed

    Birch, G F; Lean, J; Gunns, T

    2015-06-01

    Sydney estuary (Australia), like many urbanised waterways, is degraded due to an extended history of anthropogenic activity. Two major sources of contamination to this estuary are discharge by former shoreline industries and historic and contemporary catchment stormwater. The objectives of the present study were to document changes in shoreline land use from European settlement to the present day and determine the influence of this trend on the metal content of adjacent estuarine sediments. Temporal analysis of land use for seven time horizons between 1788 and 2010 showed rapid expansion of industry along much of the Sydney estuary foreshore soon after European settlement due to the benefits of easy and inexpensive access and readily available water for cooling and power. Shoreline industry attained maximum development in 1978 (32-km length) and declined rapidly to the present-day (9-km length) through redevelopment of industrial sites into medium- to high-density, high-value residential housing. Cores taken adjacent to 11 long-term industrial sites showed that past industrial practices contributed significantly to contamination of estuarine sediment. Subsurface metal concentrations were up to 35 times that of present-day surface sediment and over 100 times greater than natural background concentrations. Sedimentation rates for areas adjacent to shoreline industry were between 0.6 and 2.5 cm/year, and relaxation times were estimated at 50 to 100 years. Natural relaxation and non-disturbance of sediments may be the best management practice in most locations. PMID:25937494

  7. Satellite assisted aerosol correlation in a sequestered CO2 leakage controlled site

    NASA Astrophysics Data System (ADS)

    Landulfo, Eduardo; da Silva Lopes, Fábio J.; Nakaema, Walter M.; de Medeiros, José A. G.; Moreira, Andrea

    2014-10-01

    Currently one of the main challenges in CO2 storage research is to grant the development, testing and validation of accurate and efficient Measuring, Monitoring and Verification (MMV) techniques to be deployed at the final storage site, targeting maximum storage efficiency at the minimal leakage risk levels. For such task a mimetic sequestration site has been deployed in Florianopolis, Brazil, in order to verify the performance of monitoring plataforms to detect and quantify leakages of ground injected CO2, namely a Cavity Ring Down System (CRDS) - Los Gatos Research - an Eddy Covariance System (Campbell Scientific and Irgason) and meteorological tower for wind, humidity, precipitation and temperature monitoring onsite. The measurement strategy for detecting CO2 leakages can be very challenging since environmental and phytogenic influence can be very severe and play a role on determining if the values measured are unambiguous or not. One external factor to be considered is the amount of incoming solar radiation which will be the driving force for the whole experimental setup and following this reasoning the amount of aerosols in the atmospheric column can be a determinant factor influencing the experimental results. Thus the investigation of measured fluxes CO2 and its concentration with the aforementioned experimental instruments and their correlation with the aerosol data should be taken into account by means of satellite borne systems dedicated to measure aerosol vertical distribution and its optical properties, in this study we have selected CALIPSO and MODIS instrumentation to help on deriving the aerosol properties and CO2 measurements.

  8. [Systematic review of surgical gowns in the control of contamination/surgical site infection].

    PubMed

    Burgatti, Juliane Cristina; Lacerda, Rúbia Aparecida

    2009-03-01

    Surgical scrubs are made with both fabric and non-fabric material. The study aimed to observe whether there is scientific evidence, according to the systematic review, that supports the practice of wearing scrubs in surgeries, according to the material they are made of. Basic intervention studies were considered, which investigated contamination and/or infection of the surgical site with the use of either reusable or single-use surgical scrubs, using people submitted to surgeries as the study population, either in real or simulated situations, at any period, without any language limitations. The strategy of searching electronic databases was used to find studies. With this, difficulties in isolating the object of intervention from countless other factors that can interfere in the outcomes were identified in studies of this type. Two studies (E1 and E2) showed strong evidence for the recommendation. In conclusion, there is no difference in contamination and infection of the surgical site between fabric and non-fabric scrubs. PMID:19437878

  9. Environmental control technology survey of selected US strip mining sites. Volume 2B. Alabama. Water quality impacts and overburden chemistry of Alabama study site

    SciTech Connect

    Henricks, J D; Bogner, J E; Olsen, R D; Schubert, J P; Sobek, A A; Johnson, D O

    1980-05-01

    As part of a program to examine the ability of existing control technologies to meet federal guidelines for the quality of aqueous effluents from coal mines, an intensive study of water, coal, and overburden chemistry was conducted at a surface coal mine in Alabama from May 1976 through July 1977. Sampling sites included the pit sump, a stream downgrade from the mine, the discharge from the water treatment facility, and a small stream outside the mine drainage. Water samples were collected every two weeks by Argonne subcontractors at the Alabama Geological Survey and analysed for the following parameters: specific conductance, pH, temperature, acidity, bicarbonate, carbonate, chloride, total dissolved solids, suspended solids, sulfate, and 20 metals. Analysis of the coal and overburden shows that no potential acid problem exists at this mine. Water quality is good in both streams sampled, and high levels of dissolved elements are found only in water collected from the pit sump. The mine effluent is in compliance with Office of Surface Mining water quality standards.

  10. Effects of nitrogen oxide emission controls on Eastern US surface ozone: A comparison between urban cores and rural background sites

    NASA Astrophysics Data System (ADS)

    Rieder, Harald E.; Fiore, Arlene M.

    2016-04-01

    Nitrogen oxide (NOx) emission controls have led to improved air quality over the past two decades, particularly over the Eastern US. In recent work we quantified the effects of the efforts to abate surface ozone (O3) pollution under the NOx State Implementation Plan (NOx SIP Call) for Eastern US background sites (available from the Clean Air Status and Trends Network (CASTNET)) using methods from statistical extreme value theory (Rieder et al., 2013). Our analysis showed that the number of summer (JJA) days above the US national ambient air quality standard (NAAQS) declined on average by a factor of two between 1988-1998 and 1999-2009 and that probabilistic 1-yr O3 return values declined by about 10 ppb between these two time periods. Here we extend the analysis to observations available from the US EPA Air Quality System (AQS), comprising sites ranging from polluted urban cores to rural sites. We focus on changes in (i) the seasonal and annual average number of days with maximum daily 8-hour average surface O3 above the NAAQS and (ii) probabilistic O3 return values following the NOx SIP Call. Particular focus is given on similarities and differences in surface O3 responses on regional to local level and on contrasting urban cores and rural background sites. References: Rieder H.E., Fiore A.M., Polvani L.M., Lamarque J.-F., Fang Y. (2013): Changes in the frequency and return level of high ozone pollution events over the Eastern United States following emission controls, Environ. Res. Lett., 8, 014012, 2013.

  11. ATPase active-site electrostatic interactions control the global conformation of the 100 kDa SecA translocase

    PubMed Central

    Kim, Dorothy M.; Zheng, Haiyan; Huang, Yuanpeng J.; Montelione, Gaetano T.; Hunt, John F.

    2013-01-01

    SecA is an intensively studied mechanoenzyme that uses ATP hydrolysis to drive processive extrusion of secreted proteins through a protein-conducting channel in the cytoplasmic membrane of eubacteria. The ATPase motor of SecA is strongly homologous to that in DEAD-box RNA helicases. It remains unclear how local chemical events in its ATPase active site control the overall conformation of an ~100 kDa multidomain enzyme and drive protein transport. In this paper, we use biophysical methods to establish that a single electrostatic charge in the ATPase active site controls the global conformation of SecA. The enzyme undergoes an ATP-modulated endothermic conformational transition (ECT) believed to involve similar structural mechanics to the protein transport reaction. We have characterized the effects of an isosteric glutamate-to-glutamine mutation in the catalytic base, which mimics the immediate electrostatic consequences of ATP hydrolysis in the active site. Calorimetric studies demonstrate that this mutation facilitates the ECT in E. coli SecA and triggers it completely in B. subtilis SecA. Consistent with the substantial increase in entropy observed in the course of the ECT, hydrogen-deuterium exchange mass spectrometry demonstrates that it increases protein backbone dynamics in domain-domain interfaces at remote locations from the ATPase active site. The catalytic glutamate is one of ~250 charged amino acids in SecA, and yet neutralization of its sidechain charge is sufficient to trigger a global order-disorder transition in this 100 kDa enzyme. The intricate network of structural interactions mediating this effect couples local electrostatic changes during ATP hydrolysis to global conformational and dynamic changes in SecA. This network forms the foundation of the allosteric mechanochemistry that efficiently harnesses the chemical energy stored in ATP to drive complex mechanical processes. PMID:23167435

  12. Flow and transport within a coastal aquifer adjacent to a stratified water body

    NASA Astrophysics Data System (ADS)

    Oz, Imri; Yechieli, Yoseph; Eyal, Shalev; Gavrieli, Ittai; Gvirtzman, Haim

    2016-04-01

    The existence of a freshwater-saltwater interface and the circulation flow of saltwater beneath the interface is a well-known phenomenon found at coastal aquifers. This flow is a natural phenomenon that occurs due to density differences between fresh groundwater and the saltwater body. The goals of this research are to use analytical, numerical, and physical models in order to examine the configuration of the freshwater-saltwater interface and the density-driven flow patterns within a coastal aquifer adjacent to long-term stratified saltwater bodies (e.g. meromictic lake). Such hydrological systems are unique, as they consist of three different water types: the regional fresh groundwater, and low and high salinity brines forming the upper and lower water layers of the stratified water body, respectively. This research also aims to examine the influence of such stratification on hydrogeological processes within the coastal aquifer. The coastal aquifer adjacent to the Dead Sea, under its possible future meromictic conditions, serves as an ideal example to examine these processes. The results show that adjacent to a stratified saltwater body three interfaces between three different water bodies are formed, and that a complex flow system, controlled by the density differences, is created, where three circulation cells are developed. These results are significantly different from the classic circulation cell that is found adjacent to non-stratified water bodies (lakes or oceans). In order to obtain a more generalized insight into the groundwater behavior adjacent to a stratified water body, we used the numerical model to perform sensitivity analysis. The hydrological system was found be sensitive to three dimensionless parameters: dimensionless density (i.e. the relative density of the three water bodies'); dimensionless thickness (i.e. the ratio between the relative thickness of the upper layer and the whole thickness of the lake); and dimensionless flux. The results

  13. Robust Classification and Segmentation of Planar and Linear Features for Construction Site Progress Monitoring and Structural Dimension Compliance Control

    NASA Astrophysics Data System (ADS)

    Maalek, R.; Lichti, D. D.; Ruwanpura, J.

    2015-08-01

    The application of terrestrial laser scanners (TLSs) on construction sites for automating construction progress monitoring and controlling structural dimension compliance is growing markedly. However, current research in construction management relies on the planned building information model (BIM) to assign the accumulated point clouds to their corresponding structural elements, which may not be reliable in cases where the dimensions of the as-built structure differ from those of the planned model and/or the planned model is not available with sufficient detail. In addition outliers exist in construction site datasets due to data artefacts caused by moving objects, occlusions and dust. In order to overcome the aforementioned limitations, a novel method for robust classification and segmentation of planar and linear features is proposed to reduce the effects of outliers present in the LiDAR data collected from construction sites. First, coplanar and collinear points are classified through a robust principal components analysis procedure. The classified points are then grouped using a robust clustering method. A method is also proposed to robustly extract the points belonging to the flat-slab floors and/or ceilings without performing the aforementioned stages in order to preserve computational efficiency. The applicability of the proposed method is investigated in two scenarios, namely, a laboratory with 30 million points and an actual construction site with over 150 million points. The results obtained by the two experiments validate the suitability of the proposed method for robust segmentation of planar and linear features in contaminated datasets, such as those collected from construction sites.

  14. Opioids and TRPV1 in the peripheral control of neuropathic pain--Defining a target site in the injured nerve.

    PubMed

    Labuz, Dominika; Spahn, Viola; Celik, Melih Özgür; Machelska, Halina

    2016-02-01

    Targeting peripheral neuropathic pain at its origin may prevent the development of hypersensitivity. Recently we showed this can be mediated by opioid receptors at the injured nerve trunk. Here, we searched for the most relevant peripheral site to block transient receptor potential vanilloid 1 (TRPV1), and investigated analgesic interactions between TRPV1 and opioids in neuropathy. In a chronic constriction injury (CCI) of the sciatic nerve in mice, we assessed the effects of μ-, δ- and κ-opioid receptor agonists and TRPV1 antagonist (SB366791) injected at the CCI site or into the injured nerve-innervated paw on spontaneous paw lifting, heat and mechanical sensitivity. We also examined TRPV1 expression in total membrane and plasma membrane fractions from nerves and paws. We found that opioids and SB366791 co-injected in per se nonanalgesic doses at the CCI site or into the paw diminished heat and mechanical sensitivity. SB366791 alone dose-dependently alleviated heat and mechanical sensitivity. TRPV1 blockade in the paw was more effective than at the CCI site. None of the treatments diminished spontaneous paw lifting. TRPV1 expression analysis suggests that the levels of functional TRPV1 do not critically determine the TRPV1 antagonist-mediated analgesia. Together, the identification of the primary action site in damaged nerves is crucial for effective pain control. Contrary to opioids, the TRPV1 blockade in the injured nerve peripheral terminals, rather than at the nerve trunk, appears promising against heat pain. Opioid/TRPV1 antagonist combinations at both locations partially reduced neuropathy-triggered heat and mechanical pain. PMID:26453963

  15. VERIFICATION OF SIMPLIFIED PROCEDURES FOR SITE- SPECIFIC SO2 AND NOX CONTROL COST ESTIMATES

    EPA Science Inventory

    The report documents results of an evaluation to verify the accuracy of simplified procedures for estimating sulfur dioxide (S02) and nitrogen oxides (NOx) retrofit control costs and performance for 200 502-emitting coal-fired power plants in the 31-state eastern region. nitially...

  16. 76 FR 175 - Faurecia Emissions Control Technologies Including On-Site Leased Workers From Adecco Employment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-03

    ... July 1, 2010 (75 FR 38137). At the request of the State agency, the Department reviewed the... corporate shared services such as information technology, financial, human resources, and purchasing support... Employment and Training Administration TA-W-73,609 Faurecia Emissions Control Technologies Including...

  17. VERIFICATION OF SIMPLIFIED PROCEDURES FOR SITE-SPECIFIC SO2 AND NOX CONTROL COST ESTIMATES

    EPA Science Inventory

    The report documents results of an evaluation to verify the accuracy of simplified procedures for estimating sulfur dioxide (S02) and nitrogen oxides (NOx) retrofit control costs and performance for 200 502-emitting coal-fired power plants in the 31-state eastern region. nitially...

  18. Remote robot manipulator coupled with remote-controlled guide vehicle for soil sampling in hazardous waste sites

    NASA Astrophysics Data System (ADS)

    Kim, Kiho

    The important initial step for remediation of hazardous waste is contaminant analysis since the cleanup operation can not begin until the contaminants in hazardous waste sites have been clearly identified. Ames Laboratory, one of the U.S. Department of Energy sites, has developed a robotic sampling system for automation of real-time contaminant analysis in situ which will provide the advantage of lowering the cost per sample, eliminating personnel exposure to hazardous environments, and allowing quicker results. Successful accomplishment of real-time contaminant analysis will require a remote manipulator to perform the sampling tasks in remote and unstructured surroundings, and a remote-controlled guide vehicle to move a remote manipulator into the desired sampling location. This thesis focuses on the design and construction of a remote-controlled guide vehicle to move the robotic sampling system into the contaminated field to obtain soil samples at the desired locations, the development of an integrated dynamic model of a remote manipulator, the identification of dynamic parameters in the integrated dynamic model, and the design of a mobile robotic sampling system. A four-wheeled vehicle prototype has been constructed and its performance tested manually in the field to verify the design requirements. To remotely control the vehicle, mechanical requirements to activate the brake, throttle, transmission, and steering linkages were determined based on experimental results. A teleoperated control utilizing hundred feet long umbilical cords was first employed to remotely control the vehicle. Next, the vehicle was modified to remotely operate in the field by radio control without the aid of long umbilical cords, satisfying all the design specifications. To reduce modeling error in the robotic system, the integrated dynamic system comprised of a remote manipulator (located on a trailer pulled by the remote-controlled guide vehicle) and its drive system has been modeled

  19. Molecular Level Control Through Dual Site Participation Using Bimetallic Catalysts - Final Report

    SciTech Connect

    d'Itri, Julie, L.; Kovalchuk, Vladimir, I.

    2010-02-08

    The overall goal of this research program was to explore the hypothesis that it is possible to design a bimetallic surface such that each metal catalyzes different elementary reaction steps in an overall reaction pathway. A corollary to this hypothesis is that the different ensemble size requirements for an elementary reaction step can be used to force an elementary reaction step to occur on only one of the metals. The research program involved a combination of materials synthesis, chemical kinetics experiments, spectroscopic studies and computational investigations. The major outcome of this research program was the development and dissemination of the Dual Site Model, for which chlorocarbon reactions in the presence of hydrogen were used as model systems.

  20. Management of pediatric skin-graft donor sites: a randomized controlled trial of three wound care products.

    PubMed

    Brenner, Maria; Hilliard, Carol; Peel, Glynis; Crispino, Gloria; Geraghty, Ruth; OʼCallaghan, Gill

    2015-01-01

    Skin grafts are used to treat many types of skin defects in children, including burns, traumatic wounds, and revision of scars. The objective of this prospective randomized controlled trial was to compare the effectiveness of three dressing types for pediatric donor sites: foam, hydrofiber, and calcium alginate. Children attending a pediatric Burns & Plastics Service from October 2010 to March 2013, who required a split-skin graft, were recruited to the trial. Patients were randomly assigned to the two experimental groups, foam or hydrofiber, and to the control group, calcium alginate. Data were gathered on the management of exudate, assessment of pain, time to healing, and infection. Fifty-seven children aged 1 to 16 years (mean = 4.9 years) were recruited to the trial. Fifty-six patients had evaluable data and one participant from the control group was lost to follow-up. Most children required skin grafting for a burn injury (78%). The median size of the donor site was 63.50 cm (8-600 cm). There was a statistically significant difference in time to healing across the three dressing groups (x [2, n = 56] = 6.59, P = .037). The calcium alginate group recorded a lower median value of days to healing (median = 7.5 days) compared to the other two groups, which recorded median values of 8 days (hydrofiber) and 9.5 days (foam). The greatest leakage of exudate, regardless of dressing type, occurred on day 2 after grafting. No statistically significant difference was found in leakage of exudate, pain scores, or infection rates across the three groups. Calcium alginate emerged as the optimum dressing for pediatric donor site healing in this trial. PMID:25185932

  1. Controlling factors of biosphere-atmosphere ammonia exchange at a semi-natural peatland site

    NASA Astrophysics Data System (ADS)

    Brummer, C.; Richter, U.; Smith, J. J.; Delorme, J. P.; Kutsch, W. L.

    2014-12-01

    Recent advancements in laser spectrometry offer new opportunities to investigate net biosphere-atmosphere exchange of ammonia. During a three month field campaign from February to May 2014, we tested the performance of a quantum cascade laser within an eddy-covariance setup. The laser was operated at a semi-natural peatland site that is surrounded by highly fertilized agricultural land and intensive livestock production (~1 km distance). Ammonia concentrations were highly variable between 2 and almost 100 ppb with an average value of 15 ppb. Different concentration patterns could be identified. The variability was closely linked to the timing of management practices and the prevailing local climate, particularly wind direction, temperature and surface wetness with the latter indicating higher non-stomatal uptake under wet conditions leading to decreased concentrations. Average ammonia fluxes were around -15 ng N m-2 s-1 at the beginning of the campaign in February and shifted towards a neutral average exchange regime of -1 to 0 ng N m-2 s-1 in April and May. Intriguingly, during the time of decreasing ammonia uptake, concentrations were considerably rising, which clearly indicated N saturation in the predominant vegetation such as bog heather, purple moor-grass, and cotton grass. The cumulative net uptake for the period of investigation was ~300 g N ha-1. This stresses the importance of a thorough method inter-comparison, e.g. with denuder systems in combination with dry deposition modeling. As previous results from the latter methods showed an annual uptake of ~9 kg N ha-1 for the same site, the implementation of adequate ammonia compensation point parameterizations become crucial in surface-atmosphere exchange schemes for bog vegetation. Through their high temporal resolution, robustness and continuous measurement mode, quantum cascade lasers will help assessing the effects of atmospheric N loads to vulnerable N-limited ecosystems such as peatlands.

  2. Combinatorial control of cyclin B1 nuclear trafficking through phosphorylation at multiple sites.

    PubMed

    Yang, J; Song, H; Walsh, S; Bardes, E S; Kornbluth, S

    2001-02-01

    Entry into mitosis is regulated by the Cdc2 kinase complexed to B-type cyclins. We and others recently reported that cyclin B1/Cdc2 complexes, which appear to be constitutively cytoplasmic during interphase, actually shuttle continually into and out of the nucleus, with the rate of nuclear export exceeding the import rate (). At the time of entry into mitosis, the import rate is increased, whereas the export rate is decreased, leading to rapid nuclear accumulation of Cdc2/cyclin B1. Although it has recently been reported that phosphorylation of 4 serines within cyclin B1 promotes the rapid nuclear translocation of Cdc2/cyclin B1 at G(2)/M, the role that individual phosphorylation sites play in this process has not been examined (, ). We report here that phosphorylation of a single serine residue (Ser(113) of Xenopus cyclin B1) abrogates nuclear export of cyclin B1. This serine lies directly within the cyclin B1 nuclear export sequence and, when phosphorylated, prevents binding of the nuclear export factor, CRM1. In contrast, analysis of phosphorylation site mutants suggests that coordinate phosphorylation of all 4 serines (94, 96, 101, and 113) is required for the accelerated nuclear import of cyclin B1/Cdc2 characteristic of G(2)/M. Additionally, binding of cyclin B1 to importin-beta, the factor known to be responsible for the slow interphase nuclear entry of cyclin B1, appears to be unaffected by the phosphorylation state of cyclin B. These data suggest that a distinct import factor must be recruited to enhance nuclear entry of Cdc2/cyclin B1 at the G(2)/M transition. PMID:11060306

  3. Integration of Nevada Test Site (NTS) Work Control Programs and Incorporating Integrated Safety Management (ISM) into Activity Level Work Planning and Control

    SciTech Connect

    Mike Kinney and Kevin Breen

    2008-08-30

    This session will examine a method developed by Federal and Contractor personnel at the Nevada Site Office (NSO) to improve the planning and execution of work activities utilizing an Activity Level Work Control process in response to Defense Nuclear Facilities Safety Board (DNFSB) Recommendation 2004-1, Oversight of Complex, High-Hazard Nuclear Operations. The process was initially developed during Fiscal Year (FY) 2007, and implementation is commencing during the fourth quarter of FY 2008. This process will significantly enhance the flexibility and the appropriate rigor in the performance of work activities.

  4. Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage

    PubMed Central

    Ethier, Sylvain; Schmeing, T. Martin; Dostie, Josée; Pelletier, Jerry

    2014-01-01

    The clustered regularly interspaced short palindromic repeat (CRISPR)-associated enzyme Cas9 is an RNA-guided nuclease that has been widely adapted for genome editing in eukaryotic cells. However, the in vivo target specificity of Cas9 is poorly understood and most studies rely on in silico predictions to define the potential off-target editing spectrum. Using chromatin immunoprecipitation followed by sequencing (ChIP-seq), we delineate the genome-wide binding panorama of catalytically inactive Cas9 directed by two different single guide (sg) RNAs targeting the Trp53 locus. Cas9:sgRNA complexes are able to load onto multiple sites with short seed regions adjacent to 5′NGG3′ protospacer adjacent motifs (PAM). Yet among 43 ChIP-seq sites harboring seed regions analyzed for mutational status, we find editing only at the intended on-target locus and one off-target site. In vitro analysis of target site recognition revealed that interactions between the 5′ end of the guide and PAM-distal target sequences are necessary to efficiently engage Cas9 nucleolytic activity, providing an explanation for why off-target editing is significantly lower than expected from ChIP-seq data. PMID:25275497

  5. Wavefront control of the large optics test and integration site (LOTIS) 6.5m collimator.

    PubMed

    West, Steven C; Bailey, Samuel H; Burge, James H; Cuerden, Brian; Hagen, Jeff; Martin, Hubert M; Tuell, Michael T

    2010-06-20

    The LOTIS Collimator provides scene projection within a 6.5m diameter collimated beam used for optical testing research in air and vacuum. Diffraction-limited performance (0.4 to 5microm wavelength) requires active wavefront control of the alignment and primary mirror shape. A hexapod corrects secondary mirror alignment using measurements from collimated sources directed into the system with nine scanning pentaprisms. The primary mirror shape is controlled with 104 adjustable force actuators based on figure measurements from a center-of-curvature test. A variation of the Hartmann test measures slopes by monitoring the reflections from 36 small mirrors bonded to the optical surface of the primary mirror. The Hartmann source and detector are located at the f/15 Cassegrain focus. Initial operation has demonstrated a closed-loop 110 nm rms wavefront error in ambient air over the 6.5m collimated beam. PMID:20563205

  6. Wavefront control of the Large Optics Test and Integration Site (LOTIS) 6.5m Collimator

    SciTech Connect

    West, Steven C.; Bailey, Samuel H.; Burge, James H.; Cuerden, Brian; Hagen, Jeff; Martin, Hubert M.; Tuell, Michael T.

    2010-06-20

    The LOTIS Collimator provides scene projection within a 6.5m diameter collimated beam used for optical testing research in air and vacuum. Diffraction-limited performance (0.4 to 5{mu}m wavelength) requires active wavefront control of the alignment and primary mirror shape. A hexapod corrects secondary mirror alignment using measurements from collimated sources directed into the system with nine scanning pentaprisms. The primary mirror shape is controlled with 104 adjustable force actuators based on figure measurements from a center-of-curvature test. A variation of the Hartmann test measures slopes by monitoring the reflections from 36 small mirrors bonded to the optical surface of the primary mirror. The Hartmann source and detector are located at the f/15 Cassegrain focus. Initial operation has demonstrated a closed-loop 110nmrms wavefront error in ambient air over the 6.5mcollimated beam.

  7. Tunable translational control using site-specific unnatural amino acid incorporation in Escherichia coli

    PubMed Central

    2015-01-01

    Translation of target gene transcripts in Escherichia coli harboring UAG amber stop codons can be switched on by the amber-codon-specific incorporation of an exogenously supplied unnatural amino acid, 3-iodo-L-tyrosine. Here, we report that this translational switch can control the translational efficiency at any intermediate magnitude by adjustment of the 3-iodo-L-tyrosine concentration in the medium, as a tunable translational controller. The translational efficiency of a target gene reached maximum levels with 10−5 M 3-iodo-L-tyrosine, and intermediate levels were observed with suboptimal concentrations (approximately spanning a 2-log10 concentration range, 10−7–10−5 M). Such intermediate-level expression was also confirmed in individual bacteria. PMID:25945307

  8. AmeriFlux US-ARb ARM Southern Great Plains burn site- Lamont

    SciTech Connect

    Torn, Margaret

    2016-01-01

    This is the AmeriFlux version of the carbon flux data for the site US-ARb ARM Southern Great Plains burn site- Lamont. Site Description - The ARM SGP Burn site is located in the native tallgrass prairies of the USDA Grazinglands Research Laboratory near El Reno, OK. One of two adjacent 35 ha plots, the US-ARb plot was burned on 2005/03/08. The second plot, US-ARc, was left unburned as the control for experimental purposes. Aside from 2005, the region evaded burning activities for at least 15 years. Current disturbances consist of only light grazing activities.

  9. Optimal control theory determination of feasible return-to-launch-site aborts for the HL-20 Personnel Launch System vehicle

    NASA Astrophysics Data System (ADS)

    Dutton, Kevin E.

    1994-07-01

    The personnel launch system (PLS) being studied by NASA is a system to complement the space shuttle and provide alternative access to space. The PLS consists of a manned spacecraft launched by an expendable launch vehicle (ELV). A candidate for the manned spacecraft is the HL-20 lifting body. In the event of an ELV malfunction during the initial portion of the ascent trajectory, the HL-20 will separate from the rocket and perform an unpowered return to launch site (RTLS) abort. This work details an investigation, using optimal control theory, of the RTLS abort scenario. The objective of the optimization was to maximize final altitude. With final altitude as the cost function, the feasibility of an RTLS abort at different times during the ascent was determined. The method of differential inclusions was used to determine the optimal state trajectories, and the optimal controls were then calculated from the optimal states and state rates.

  10. Optimal control theory determination of feasible return-to-launch-site aborts for the HL-20 Personnel Launch System vehicle

    NASA Technical Reports Server (NTRS)

    Dutton, Kevin E.

    1994-01-01

    The personnel launch system (PLS) being studied by NASA is a system to complement the space shuttle and provide alternative access to space. The PLS consists of a manned spacecraft launched by an expendable launch vehicle (ELV). A candidate for the manned spacecraft is the HL-20 lifting body. In the event of an ELV malfunction during the initial portion of the ascent trajectory, the HL-20 will separate from the rocket and perform an unpowered return to launch site (RTLS) abort. This work details an investigation, using optimal control theory, of the RTLS abort scenario. The objective of the optimization was to maximize final altitude. With final altitude as the cost function, the feasibility of an RTLS abort at different times during the ascent was determined. The method of differential inclusions was used to determine the optimal state trajectories, and the optimal controls were then calculated from the optimal states and state rates.

  11. Daytime wind valleys adjacent to the Great Salt Lake

    SciTech Connect

    Stone, G.L. ); Hoard, D.E. )

    1990-01-01

    In 1986 Los Alamos National Laboratory was engaged by the US Army to study the meteorological aspects of emergency preparedness at several sites where toxic materials are stored and handled. The project included a series of tracer and meteorological field experiments in the vicinity of the Tooele Army Depot. These experiments generated a large data set for validating numerical simulations and for empirical analyses of the local meteorology. This paper discusses the main characteristics of the daytime, up-valley flow at the Utah site, including frequency of occurrence, horizontal and vertical structure, and temporal evolution. Some parameters controlling the variability in onset time for up-valley flow are identified, and an empirical forecasting scheme is discussed. 16 refs., 7 figs.

  12. Subsoil heterogeneities controlling porewater contaminant mass and microbial diversity at a site with a complex pollution history

    NASA Astrophysics Data System (ADS)

    Puigserver, Diana; Carmona, José M.; Cortés, Amparo; Viladevall, Manuel; Nieto, José M.; Grifoll, Magdalena; Vila, Joaquim; Parker, Beth L.

    2013-01-01

    This study seeks to improve our understanding of the conceptual model of pollutant transport and fate in cases of DNAPL contamination at sites with a complex contamination history. The study was carried out in an unconfined aquifer of alluvial fans in the Tarragona Petrochemical Complex (Spain). Two boreholes were drilled and continuous cores were recovered in order to carry out a detailed core description at centimeter scale and a comprehensive sampling of borehole cores. The biogeochemical heterogeneity at these sites is controlled by the conjunction of lithological, hydrochemical and microbiological heterogeneities. Biodegradation processes of contaminant compounds take place not only at the level of the dissolved fraction in the aquifer but also at the level of the fraction retained in the fine, less conductive materials as shown by the biodegradation haloes of parent and metabolite compounds. Sampling the low-conductivity levels also allowed us to identify compounds, e.g. BTEX, that are the remaining traces of the passage of old contaminant plumes whose sources no longer exist. This enabled us to describe past biogeochemical processes and to partially account for the processes occurring today. Transition zones, characterized by numerous textural changes, constitute ecotones whose biostimulation could be effective in promoting the acceleration of the remediation of the multiple pollution at these sites.

  13. Subsoil heterogeneities controlling porewater contaminant mass and microbial diversity at a site with a complex pollution history.

    PubMed

    Puigserver, Diana; Carmona, José M; Cortés, Amparo; Viladevall, Manuel; Nieto, José M; Grifoll, Magdalena; Vila, Joaquim; Parker, Beth L

    2013-01-01

    This study seeks to improve our understanding of the conceptual model of pollutant transport and fate in cases of DNAPL contamination at sites with a complex contamination history. The study was carried out in an unconfined aquifer of alluvial fans in the Tarragona Petrochemical Complex (Spain). Two boreholes were drilled and continuous cores were recovered in order to carry out a detailed core description at centimeter scale and a comprehensive sampling of borehole cores. The biogeochemical heterogeneity at these sites is controlled by the conjunction of lithological, hydrochemical and microbiological heterogeneities. Biodegradation processes of contaminant compounds take place not only at the level of the dissolved fraction in the aquifer but also at the level of the fraction retained in the fine, less conductive materials as shown by the biodegradation haloes of parent and metabolite compounds. Sampling the low-conductivity levels also allowed us to identify compounds, e.g. BTEX, that are the remaining traces of the passage of old contaminant plumes whose sources no longer exist. This enabled us to describe past biogeochemical processes and to partially account for the processes occurring today. Transition zones, characterized by numerous textural changes, constitute ecotones whose biostimulation could be effective in promoting the acceleration of the remediation of the multiple pollution at these sites. PMID:23149156

  14. Characterization of a DNA binding activity in DNAse I hypersensitive site 4 of the human globin locus control region.

    PubMed Central

    Walters, M; Kim, C; Gelinas, R

    1991-01-01

    A portion of the beta-globin Locus Control Region (LCR), which included DNAse I hypersensitive site 4 (HS4), was analyzed for its interactions with nuclear extracts and its contribution to LCR activity in a functional assay. In gel retardation assays, a short fragment from HS4 formed complexes with nuclear extracts from both erythroid and nonerythroid cells, and a core protected sequence 5'GACTGGC3' was revealed by DNAse I protection and methylation interference studies. This sequence resembles the binding sites of CCAAT-family members. Purified CP-2 but not CP-1 was shown to bind this HS4 sequence in a gel shift reaction, suggesting that the HS4 binding activity shares some sequence specificity with the CCAAT-factor family. Utilizing a transient expression assay in murine erythroleukemia cells, steady-state RNA levels were measured from pairs of LCR constructs linked to distinguishable beta-globin reporter genes. A short DNA fragment from HS4 which included the binding site for this novel binding activity accounted for most of the contribution to high level expression made by the entire HS4 region. Images PMID:1923823

  15. Quantum phase transition of a Bose gas in a lattice with a controlled number of atoms per site

    NASA Astrophysics Data System (ADS)

    Du, Xu

    2005-05-01

    We have studied the superfluid-Mott insulator quantum phase transition [1] of a gas of ^87Rb atoms in an optical lattice. We are able to prepare the gas with a controllable number of one, two, or three atoms per lattice site, as verified with photoassociation spectroscopy. We measure momentum distributions using standard time-of-flight imaging techniques. These are similar to those of ref. [1], and exhibit narrow peaks at moderate lattice strengths. We find that the width of these peaks increases for lattice heights greater than about 13 times the recoil energy [2], and we observe interesting differences in this behavior, depending on the number of atoms per site. The data suggest that the quantum phase transition occurs at higher lattice strength with larger site occupation. We acknowledge the support of this work by the R. A. Welch Foundation, The N. S. F., and the D.O.E. Quantum Optics Initiative. [1] Markus Greiner et al., Nature 415, 39 (2002). [2] Thilo St"oferle et al., Phys. Rev. Lett. 92, 130403 (2004).

  16. FACT plays a major role in histone dynamics affecting VSG expression site control in Trypanosoma brucei.

    PubMed

    Denninger, Viola; Rudenko, Gloria

    2014-11-01

    Chromatin remodelling is involved in the transcriptional regulation of the RNA polymerase I transcribed variant surface glycoprotein (VSG) expression sites (ESs) of Trypanosoma brucei. We show that the T. brucei FACT complex contains the Pob3 and Spt16 subunits, and plays a key role in ES silencing. We see an inverse correlation between transcription and condensed chromatin, whereby FACT knockdown results in ES derepression and more open chromatin around silent ES promoters. Derepressed ESs show increased sensitivity to micrococcal nuclease (MNase) digestion, and a decrease in histones at silent ES promoters but not telomeres. In contrast, FACT knockdown results in more histones at the active ES, correlated with transcription shut-down. ES promoters are derepressed in cells stalled at the G2/M cell cycle stage after knockdown of FACT, but not in G2/M cells stalled after knockdown of cyclin 6. This argues that the observed ES derepression is a direct consequence of histone chaperone activity by FACT at the G2/M cell cycle stage which could affect transcription elongation, rather than an indirect consequence of a cell cycle checkpoint. These experiments highlight the role of the FACT complex in cell cycle-specific chromatin remodelling within VSG ESs. PMID:25266856

  17. FACT plays a major role in histone dynamics affecting VSG expression site control in Trypanosoma brucei

    PubMed Central

    Denninger, Viola; Rudenko, Gloria

    2014-01-01

    Chromatin remodelling is involved in the transcriptional regulation of the RNA polymerase I transcribed variant surface glycoprotein (VSG) expression sites (ESs) of Trypanosoma brucei. We show that the T. brucei FACT complex contains the Pob3 and Spt16 subunits, and plays a key role in ES silencing. We see an inverse correlation between transcription and condensed chromatin, whereby FACT knockdown results in ES derepression and more open chromatin around silent ES promoters. Derepressed ESs show increased sensitivity to micrococcal nuclease (MNase) digestion, and a decrease in histones at silent ES promoters but not telomeres. In contrast, FACT knockdown results in more histones at the active ES, correlated with transcription shut-down. ES promoters are derepressed in cells stalled at the G2/M cell cycle stage after knockdown of FACT, but not in G2/M cells stalled after knockdown of cyclin 6. This argues that the observed ES derepression is a direct consequence of histone chaperone activity by FACT at the G2/M cell cycle stage which could affect transcription elongation, rather than an indirect consequence of a cell cycle checkpoint. These experiments highlight the role of the FACT complex in cell cycle-specific chromatin remodelling within VSG ESs. PMID:25266856

  18. Drinking water quality in Nepal's Kathmandu Valley: a survey and assessment of selected controlling site characteristics

    NASA Astrophysics Data System (ADS)

    Warner, Nathaniel R.; Levy, Jonathan; Harpp, Karen; Farruggia, Frank

    2008-03-01

    Water was sampled from over 100 sources in Nepal’s Kathmandu Valley, including municipal taps, dug wells, shallow-aquifer tube wells, deep-aquifer tube wells, and dhunge dharas (or stone spouts, public water sources that capture groundwater or surface water). Information was gathered on user preference and site and well characteristics, and water was examined for indicators of contamination from sewage, agriculture, or industry. Most problematic were total coliform and Escherichia coli bacteria, which were present in 94 and 72% of all the water samples, respectively. Contamination by nitrate, ammonia and heavy metals was more limited; nitrate and ammonia exceeded Nepali guidelines in 11 and 45% of the samples, respectively. Arsenic and mercury exceeded WHO guidelines in 7 and 10% of the samples, respectively, but arsenic never exceeded the less strict Nepali guideline. Significant differences existed in contamination levels between types of sources; dug wells and dhunge dharas, being the shallowest, were the most contaminated by bacteria and nitrate; deep-aquifer tube wells were the most contaminated by arsenic. Whereas E. coli concentrations decreased with depth, iron and ammonia concentrations increased with depth. These relationships account for people choosing to drink water with higher levels of bacterial contamination based on its superior (non-metallic) taste and appearance.

  19. NASA's Beachside Corrosion Test Site and Current Environmentally Friendly Corrosion Control Initiatives

    NASA Technical Reports Server (NTRS)

    Russell, Richard W.; Calle, Luz Marina; Johnston, Frederick; Montgomery, Eliza L.; Curran, Jerome P.; Kolody, Mark R.

    2013-01-01

    NASA began corrosion studies at the Kennedy Space Center (KSC) in 1966 during the Gemini/Apollo Programs with the evaluation of long-term corrosion protective coatings for carbon steel. KSC's Beachside Corrosion Test Site (BCTS), which has been documented by the American Society of Materials (ASM) as one of the most corrosive, naturally occurring, environments in the world, was established at that time. With the introduction of the Space Shuttle in 1981, the already highly corrosive conditions at the launch pad were rendered even more severe by the acid ic exhaust from the solid rocket boosters. In the years that followed, numerous studies have identified materials, coatings, and maintenance procedures for launch hardware and equipment exposed to the highly corrosive environment at the launch pad. This paper presents a historical overview of over 45 years of corrosion and coating evaluation studies and a description of the BCTS's current capabilities. Additionally, current research and testing programs involving chromium free coatings, environmentally friendly corrosion preventative compounds, and alternates to nitric acid passivation will be discussed.

  20. Conformational toggling controls target site choice for the heteromeric transposase element Tn7.

    PubMed

    Shi, Qiaojuan; Straus, Marco R; Caron, Jeremy J; Wang, Huasheng; Chung, Yu Seon; Guarné, Alba; Peters, Joseph E

    2015-12-15

    The bacterial transposon Tn7 facilitates horizontal transfer by directing transposition into actively replicating DNA with the element-encoded protein TnsE. Structural analysis of the C-terminal domain of wild-type TnsE identified a novel protein fold including a central V-shaped loop that toggles between two distinct conformations. The structure of a robust TnsE gain-of-activity variant has this loop locked in a single conformation, suggesting that conformational flexibility regulates TnsE activity. Structure-based analysis of a series of TnsE mutants relates transposition activity to DNA binding stability. Wild-type TnsE appears to naturally form an unstable complex with a target DNA, whereas mutant combinations required for large changes in transposition frequency and targeting stabilized this interaction. Collectively, our work unveils a unique structural proofreading mechanism where toggling between two conformations regulates target commitment by limiting the stability of target DNA engagement until an appropriate insertion site is identified. PMID:26384427

  1. PEST Control of Molecular Stripping of NFκB from DNA Transcription Sites.

    PubMed

    Potoyan, Davit A; Zheng, Weihua; Ferreiro, Diego U; Wolynes, Peter G; Komives, Elizabeth A

    2016-08-25

    We recently proposed a model for IκBα-mediated molecular stripping of NFκB from transcription sites. IκBα was shown experimentally to form a transient ternary complex with DNA-bound NFκB, but the mechanism by which the IκBα accelerates dissociation of the NFκB from the DNA was unknown. In this paper we construct and compute free energy profiles for the wild-type IκBα-mediated molecular stripping reaction of NFκB from DNA and compare with that for a mutant of IκBα bearing a charge-neutralized PEST. The differences in the free energy profile for stripping originate from the frustrated electrostatic interactions between the negatively charged PEST and the DNA. The PEST occupies two different conformations in the NFκB-IκBα binary complex, one of which occupies the DNA-binding cavity. Specific interactions with positively charged residues in the N-terminal domains of both p50 and p65 apparently draw the domains closer together hindering reassociation of DNA. Comparison with the charge-neutralized mutant reveals that all of these functional consequences result from the negative charges in the PEST sequence of IκBα. PMID:27098223

  2. Microscale Controls on the Fate of Contaminant Uranium in the Vadose Zone, Hanford Site, Washington

    SciTech Connect

    McKinley, James P.; Zachara, John M.; Liu, Chongxuan; Heald, Steve M.; Prenitzer, Brenda I.; Kempshall, Brian

    2006-04-15

    An alkaline brine containing uranyl (UO22+) leaked to the thick unsaturated zone at the Hanford Site. X-ray and electron microprobe imaging showed that the uranium was associated with a minority of clasts, specifically granitic clasts occupying less than four percent of the sediment volume. XANES analysis at micron resolution showed the uranium to be hexavalent. The uranium was precipitated in microfractures as radiating clusters of uranyl silicates, and sorbed uranium was not observed on other surfaces. Compositional determinations of the 1-3 µm precipitates were difficult, but indicated a sodium potassium uranyl silicate, likely sodium boltwoodite. Observations suggested that uranyl was removed from pore waters by diffusion and precipitation in microfractures, where dissolved silica within the granite-equilibrated solution would cause supersaturation with respect to sodium boltwoodite. This hypothesis was tested using a diffusion reaction model operating at microscale. Conditions favoring precipitation were simulated to be transient, and driven by the compositional contrast between pore and fracture space. Pore-space conditions, including alkaline pH, were eventually imposed on the microfracture environment. However, conditions favoring precipitation were prolonged within the microfracture by reaction at the silicate mineral surface to buffer pH in a solubility limiting acidic state, and to replenish dissolved silica. During this time, uranyl was additionally removed to the fracture space by diffusion from pore space. Uranyl is effectively immobilized within the microfracture environment within the presently unsaturated vadose zone.

  3. SPERTI Reactor Pit Building (PER605). Earth shielding protect adjacent Instrument ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SPERT-I Reactor Pit Building (PER-605). Earth shielding protect adjacent Instrument Cell (PER-606). Security fencing surrounds complex, to which gate entry is provided next to Guard House (PER-607). Note gravel road leading to control area. Earth-covered conduit leads from instrument cell to terminal building out of view. Photographer: R.G. Larsen. Date: June 22, 1955. INEEL negative no. 55-1701 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID

  4. Advanced Sensing and Control Techniques to Facilitate Semi-Autonomous Decommissioning of Hazardous Sites - Final Report

    SciTech Connect

    Schalkoff, R.J.

    2000-12-01

    This report summarizes work after 4 years of a 3-year project (no-cost extension of the above-referenced project for a period of 12 months granted). The fourth generation of a vision sensing head for geometric and photometric scene sensing has been built and tested. Estimation algorithms for automatic sensor calibration updating under robot motion have been developed and tested. We have modified the geometry extraction component of the rendering pipeline. Laser scanning now produces highly accurate points on segmented curves. These point-curves are input to a NURBS (non-uniform rational B-spline) skinning procedure to produce interpolating surface segments. The NURBS formulation includes quadrics as a sub-class, thus this formulation allows much greater flexibility without the attendant instability of generating an entire quadric surface. We have also implemented correction for diffuse lighting and specular effects. The QRobot joint level control was extended to a complete semi-autonomous robot control system for D and D operations. The imaging and VR subsystems have been integrated and tested.

  5. Nonconserved Ca2+/Calmodulin Binding Sites in Munc13s Differentially Control Synaptic Short-Term Plasticity

    PubMed Central

    Lipstein, Noa; Schaks, Sabine; Dimova, Kalina; Kalkhof, Stefan; Ihling, Christian; Kölbel, Knut; Ashery, Uri; Rhee, JeongSeop; Brose, Nils

    2012-01-01

    Munc13s are presynaptic proteins that mediate synaptic vesicle priming and thereby control the size of the readily releasable pool of vesicles. During high synaptic activity, Munc13-1 and its closely related homolog, ubMunc13-2, bind Ca2+/calmodulin, resulting in enhanced priming activity and in changes of short-term synaptic plasticity characteristics. Here, we studied whether bMunc13-2 and Munc13-3, two remote isoforms of Munc13-1 with a neuronal subtype-specific expression pattern, mediate synaptic vesicle priming and regulate short-term synaptic plasticity in a Ca2+/calmodulin-dependent manner. We identified a single functional Ca2+/calmodulin binding site in these isoforms and provide structural evidence that all Munc13s employ a common mode of interaction with calmodulin despite the lack of sequence homology between their Ca2+/calmodulin binding sites. Electrophysiological analysis showed that, during high-frequency activity, Ca2+/calmodulin binding positively regulates the priming activity of bMunc13-2 and Munc13-3, resulting in an increase in the size of the readily releasable pool of vesicles and subsequently in strong short-term synaptic enhancement of neurotransmission. We conclude that Ca2+/calmodulin-dependent regulation of priming activity is structurally and functionally conserved in all Munc13 proteins, and that the composition of Munc13 isoforms in a neuron differentially controls its short-term synaptic plasticity characteristics. PMID:22966208

  6. Host-Guest Binding-Site-Tunable Self-Assembly of Stimuli-Responsive Supramolecular Polymers.

    PubMed

    Yao, Hao; Qi, Miao; Liu, Yuyang; Tian, Wei

    2016-06-13

    Despite the remarkable progress made in controllable self-assembly of stimuli-responsive supramolecular polymers (SSPs), a basic issue that has not been consideration to date is the essential binding site. The noncovalent binding sites, which connect the building blocks and endow supramolecular polymers with their ability to respond to stimuli, are expected to strongly affect the self-assembly of SSPs. Herein, the design and synthesis of a dual-stimuli thermo- and photoresponsive Y-shaped supramolecular polymer (SSP2) with two adjacent β-cyclodextrin/azobenzene (β-CD/Azo) binding sites, and another SSP (SSP1) with similar building blocks, but only one β-CD/Azo binding site as a control, are described. Upon gradually increasing the polymer solution temperature or irradiating with UV light, SSP2 self-assemblies with a higher binding-site distribution density; exhibits a flower-like morphology, smaller size, and more stable dynamic aggregation process; and greater controllability for drug-release behavior than those observed with SSP1 self-assemblies. The host-guest binding-site-tunable self-assembly was attributed to the positive cooperativity generated among adjacent binding sites on the surfaces of SSP2 self-assemblies. This work is beneficial for precisely controlling the structural parameters and controlled release function of SSP self-assemblies. PMID:27167577

  7. Stratigraphic Control on CCl4 and CHCl3 Concentrations in the 200 West Area, Hanford Site

    SciTech Connect

    Winsor, Kelsey; Last, George V.

    2008-01-01

    An extensive subsurface contaminant plume of carbon tetrachloride (CCl4) is the focus of a remedial effort in the 200 West Area of Washington State’s Hanford Site. Remediation requires a high-resolution model of the region’s spatially variable lithofacies and of the effect these units have on CCl4 migration through the unconfined aquifer. To increase detail of previous models, a transect was chosen along the primary groundwater flow path in the most heavily contaminated area. Borehole logs of wells along this 3.7 km-long transect were systematized and used to create a cross section displaying lithofacies depth and continuity. Natural and spectral gamma geophysical logs were examined to pinpoint the depths of geologic units. Depth discrete concentrations of CCl4 and its reductive dechlorination product, chloroform (CHCl3), were overlain on this cross section. Comparison of stratigraphy to contaminant levels shows that peaks in CCl4 concentration occur in thin, fine-grained layers and other that fine-grained layers frequently form lower boundaries to regions of high concentration. Peaks in CCl4 concentrations are frequently located at different depths from those of CHCl3, suggesting that these concentrations are affected by dechlorination of CCl4. Transformation of CCl4 to CHCl3 appears to be more prevalent within reduced, iron-containing sediments. Influence of thin, fine-grained layers within the larger aquifer unit indicates that characterization of contamination in this locality should consider subsurface geology with at least as much resolution as provided in this study.

  8. Help, I Am Losing Control! Examining the Reporting of Sexual Harassment by Adolescents to Social Networking Sites.

    PubMed

    Van Royen, Kathleen; Poels, Karolien; Vandebosch, Heidi

    2016-01-01

    Sexual harassment is often encountered by adolescents on social networking sites (SNS). One option to cope with a situation of harassment on SNS is to alarm the provider by reporting the transgressive content. It is yet unclear what the determinants of reporting a sexual harassment situation on SNS are, as well as the subsequent actions to these reports from the part of the SNS provider. In this article, we seek to address these gaps, and in particular examine whether control-by-the-self over the situation and negative emotions play a role in the reporting of sexual harassment on SNS. Findings indicate that a low situational control-by-the-self, indirectly (namely through a higher experience of negative emotions such as anger and shame) increases the reporting of sexual harassment by the victim. Public visibility of the incident and the impossibility to remove the content reduce the situational control-by-the-self. Results further suggest that SNS providers often ignore reported situations of sexual harassment. The study concludes with suggestions for responses to reported harassment on SNS, which should be directed toward increasing behavioral control and thereby alleviating negative emotions. PMID:26779661

  9. Laplacian versus adjacency matrix in quantum walk search

    NASA Astrophysics Data System (ADS)

    Wong, Thomas G.; Tarrataca, Luís; Nahimov, Nikolay

    2016-06-01

    A quantum particle evolving by Schrödinger's equation contains, from the kinetic energy of the particle, a term in its Hamiltonian proportional to Laplace's operator. In discrete space, this is replaced by the discrete or graph Laplacian, which gives rise to a continuous-time quantum walk. Besides this natural definition, some quantum walk algorithms instead use the adjacency matrix to effect the walk. While this is equivalent to the Laplacian for regular graphs, it is different for non-regular graphs and is thus an inequivalent quantum walk. We algorithmically explore this distinction by analyzing search on the complete bipartite graph with multiple marked vertices, using both the Laplacian and adjacency matrix. The two walks differ qualitatively and quantitatively in their required jumping rate, runtime, sampling of marked vertices, and in what constitutes a natural initial state. Thus the choice of the Laplacian or adjacency matrix to effect the walk has important algorithmic consequences.

  10. Site-controlled fabrication of Ga nanodroplets by focused ion beam

    SciTech Connect

    Xu, Xingliang; Wang, Zhiming M.; Wu, Jiang; Li, Handong; Zhou, Zhihua; Wang, Xiaodong

    2014-03-31

    Ga droplets are created by focused ion beam irradiation of GaAs surface. We report that ordered Ga droplets can be formed on the GaAs surface without any implantation damage. The droplets are characterized with bigger sizes than those droplets formed on damaged area. These aligned Ga droplets are formed via the migration of Ga atoms from ion irradiation area to the edge of undamaged GaAs surface and further nucleation into droplets. The morphological evolution and size distribution of these nanodroplets are investigated systematically with different beam irradiation time and incident angles. Based on this method, well positioned Ga nanodroplets, such as chains, are achieved by using focus ion beam patterning. The controllable assembly of droplets on undamaged semiconductor surface can be used to fabricate templates, to fabricate quantum structures and quantum devices by droplet epitaxy technique.

  11. Developing standard performance testing procedures for material control and accounting components at a site

    SciTech Connect

    Scherer, Carolynn P; Bushlya, Anatoly V; Efimenko, Vladimir F; Ilyanstev, Anatoly; Regoushevsky, Victor I

    2010-01-01

    The condition of a nuclear material control and accountability system (MC&A) and its individual components, as with any system combining technical elements and documentation, may be characterized through an aggregate of values for the various parameters that determine the system's ability to perform. The MC&A system's status may be functioning effectively, marginally or not functioning based on a summary of the values of the individual parameters. This work included a review of the following subsystems, MC&A and Detecting Material Losses, and their respective elements for the material control and accountability system: (a) Elements of the MC&A Subsystem - Information subsystem (Accountancy/Inventory), Measurement subsystem, Nuclear Material Access subsystem, including tamper-indicating device (TID) program, and Automated Information-gathering subsystem; (b) Elements for Detecting Nuclear Material Loses Subsystem - Inventory Differences, Shipper/receiver Differences, Confirmatory Measurements and differences with accounting data, and TID or Seal Violations. In order to detect the absence or loss of nuclear material there must be appropriate interactions among the elements and their respective subsystems from the list above. Additionally this work includes a review of regulatory requirements for the MC&A system component characteristics and criteria that support the evaluation of the performance of the listed components. The listed components had performance testing algorithms and procedures developed that took into consideration the regulatory criteria. The developed MC&A performance-testing procedures were the basis for a Guide for MC&A Performance Testing at the material balance areas (MBAs) of State Scientific Center of the Russian Federation - Institute for Physics and Power Engineering (SSC RF-IPPE).

  12. Efficacy of burning, tillage, and biocides in controlling bacteria released at field sites and effects on indigenous bacteria and fungi.

    PubMed

    Donegan, K; Fieland, V; Fowles, N; Ganio, L; Seidler, R

    1992-04-01

    Decontamination treatments of burning and biocide application, alone and in combination with tillage, were evaluated for their ability to reduce populations of bacteria applied to the leaves of plants in field plots. In addition, the effects of these control methods on indigenous leaf and soil bacteria and fungi were assessed. Field plots of bush beans (Phaseolus vulgaris), sprayed with the bacterium Pseudomonas syringae, Pseudomonas fluorescens, or Erwinia herbicola, received the following treatments: (i) control, (ii) tillage, (iii) burning, (iv) burning plus tillage (burn-tillage), (v) Kocide (cupric hydroxide), (vi) Kocide plus tillage, (vii) Agri-Strep (streptomycin sulfate), and (viii) Agri-Strep plus tillage. Leaves and soil from the plots were sampled at 1 day before and at 1, 3, 7, 10, 14, 21, and 30 days after application of the decontamination treatments. The burn and burn-tillage treatments produced the most significant reductions in bacterial populations. The Agri-Strep treatment was more effective than the Kocide treatment in eliminating applied bacteria, but neither biocide produced consistent or persistent control. In contrast, the tillage treatment, alone or in combination with the Agri-Strep or Kocide treatments, had a short-term stimulatory effect and increased populations of applied bacteria and also levels of indigenous fungi and bacteria. Agri-Strep and Kocide treatments caused significant reductions in indigenous bacterial populations up to 14 days after application and in indigenous fungal populations on day 7 after application. Our results suggest that conventional plant disease control methods may not provide satisfactory control of genetically engineered microorganisms and indicate a need for further development of effective and selective methods to control release microorganisms at field sites. PMID:1599240

  13. Efficacy of burning, tillage, and biocides in controlling bacteria released at field sites and effects on indigenous bacteria and fungi.

    PubMed Central

    Donegan, K; Fieland, V; Fowles, N; Ganio, L; Seidler, R

    1992-01-01

    Decontamination treatments of burning and biocide application, alone and in combination with tillage, were evaluated for their ability to reduce populations of bacteria applied to the leaves of plants in field plots. In addition, the effects of these control methods on indigenous leaf and soil bacteria and fungi were assessed. Field plots of bush beans (Phaseolus vulgaris), sprayed with the bacterium Pseudomonas syringae, Pseudomonas fluorescens, or Erwinia herbicola, received the following treatments: (i) control, (ii) tillage, (iii) burning, (iv) burning plus tillage (burn-tillage), (v) Kocide (cupric hydroxide), (vi) Kocide plus tillage, (vii) Agri-Strep (streptomycin sulfate), and (viii) Agri-Strep plus tillage. Leaves and soil from the plots were sampled at 1 day before and at 1, 3, 7, 10, 14, 21, and 30 days after application of the decontamination treatments. The burn and burn-tillage treatments produced the most significant reductions in bacterial populations. The Agri-Strep treatment was more effective than the Kocide treatment in eliminating applied bacteria, but neither biocide produced consistent or persistent control. In contrast, the tillage treatment, alone or in combination with the Agri-Strep or Kocide treatments, had a short-term stimulatory effect and increased populations of applied bacteria and also levels of indigenous fungi and bacteria. Agri-Strep and Kocide treatments caused significant reductions in indigenous bacterial populations up to 14 days after application and in indigenous fungal populations on day 7 after application. Our results suggest that conventional plant disease control methods may not provide satisfactory control of genetically engineered microorganisms and indicate a need for further development of effective and selective methods to control release microorganisms at field sites. PMID:1599240

  14. Controlled, Site-Specific Functionalization of Carbon Nanotubes with Diazonium Salts

    NASA Technical Reports Server (NTRS)

    Tour, James M.

    2013-01-01

    capacities and uses. Site-specific functionalization may enable the use of nanotubes in molecular electronic applications because device functionality is critical at the cross points.

  15. Hydrologic Characteristics of Low-Impact Stormwater Control Measures at Two Sites in Northeastern Ohio, 2008-13 (USGS Scientific Investigations Report 2015-5030)

    EPA Science Inventory

    This report updates and examines hydrologic data gathered to characterize the performance of two stormwater control measures (SCM) sites in the Chagrin River watershed, Ohio. At the Sterncrest Drive site, roadside bioswales and rain gardens were used to alleviate drainage problem...

  16. On the Adjacent Eccentric Distance Sum Index of Graphs

    PubMed Central

    Qu, Hui; Cao, Shujuan

    2015-01-01

    For a given graph G, ε(v) and deg(v) denote the eccentricity and the degree of the vertex v in G, respectively. The adjacent eccentric distance sum index of a graph G is defined as ξsv(G)=∑v∈V(G)ε(v)D(v)deg(v), where D(v)=∑u∈V(G)d(u,v) is the sum of all distances from the vertex v. In this paper we derive some bounds for the adjacent eccentric distance sum index in terms of some graph parameters, such as independence number, covering number, vertex connectivity, chromatic number, diameter and some other graph topological indices. PMID:26091095

  17. Nonlinear spin wave coupling in adjacent magnonic crystals

    NASA Astrophysics Data System (ADS)

    Sadovnikov, A. V.; Beginin, E. N.; Morozova, M. A.; Sharaevskii, Yu. P.; Grishin, S. V.; Sheshukova, S. E.; Nikitov, S. A.

    2016-07-01

    We have experimentally studied the coupling of spin waves in the adjacent magnonic crystals. Space- and time-resolved Brillouin light-scattering spectroscopy is used to demonstrate the frequency and intensity dependent spin-wave energy exchange between the side-coupled magnonic crystals. The experiments and the numerical simulation of spin wave propagation in the coupled periodic structures show that the nonlinear phase shift of spin wave in the adjacent magnonic crystals leads to the nonlinear switching regime at the frequencies near the forbidden magnonic gap. The proposed side-coupled magnonic crystals represent a significant advance towards the all-magnonic signal processing in the integrated magnonic circuits.

  18. Molecular disorganization of axons adjacent to human lacunar infarcts

    PubMed Central

    Lee, Monica D.; Tung, Spencer; Vinters, Harry V.; Carmichael, S. Thomas

    2015-01-01

    Cerebral microvascular disease predominantly affects brain white matter and deep grey matter, resulting in ischaemic damage that ranges from lacunar infarcts to white matter hyperintensities seen on magnetic resonance imaging. These lesions are common and result in both clinical stroke syndromes and accumulate over time, resulting in cognitive deficits and dementia. Magnetic resonance imaging studies suggest that these lesions progress over time, accumulate adjacent to prior lesions and have a penumbral region susceptible to further injury. The pathological correlates of this adjacent injury in surviving myelinated axons have not been previously defined. In this study, we sought to determine the molecular organization of axons in tissue adjacent to lacunar infarcts and in the regions surrounding microinfarcts, by determining critical elements in axonal function: the morphology and length of node of Ranvier segments and adjacent paranodal segments. We examined post-mortem brain tissue from six patients with lacunar infarcts and tissue from two patients with autosomal dominant retinal vasculopathy and cerebral leukoencephalopathy (previously known as hereditary endotheliopathy with retinopathy, nephropathy and stroke) who accumulate progressive white matter ischaemic lesions in the form of lacunar and microinfarcts. In axons adjacent to lacunar infarcts yet extending up to 150% of the infarct diameter away, both nodal and paranodal length increase by ∼20% and 80%, respectively, reflecting a loss of normal cell-cell adhesion and signalling between axons and oligodendrocytes. Using premorbid magnetic resonance images, brain regions from patients with retinal vasculopathy and cerebral leukoencephalopathy that harboured periventricular white matter hyperintensities were selected and the molecular organization of axons was determined within these regions. As in regions adjacent to lacunar infarcts, nodal and paranodal length in white matter of these patients is

  19. Assessment of Soil Moisture and Fixatives Performance in Controlling Wind Erosion of Contaminated Soil at the Hanford Site

    SciTech Connect

    Lagos, L.E.; Gudavalli, R.K.

    2008-07-01

    During the remediation of burial grounds at the US Department of Energy's (DOE's) Hanford Site in Washington State, the dispersion of contaminated soil particles and dust is an issue that is faced by site workers on a daily basis. This contamination issue is even more of a concern when one takes into account the semi-arid characteristics of the region where the site is located. To mitigate this problem, workers at the site use a variety of engineered methods to minimize the dispersion of contaminated soil and dust particles. Once such methods is the use of water and/or suppression agents (fixatives) that stabilizes the soil prior to soil excavation, segregation, and removal activities. A primary contributor to the dispersion of contaminated soil and dust is wind soil erosion. The erosion process occurs when the wind speed exceeds a certain threshold value (threshold shear velocity), which depends on a number of factors including wind force loading, particle size, surface soil moisture, and the geometry of the soil. Thus under these circumstances the mobility of contaminated soil and generation and dispersion of particulate matter are significantly influenced by these parameters. Wind tunnel experiments were conducted at the Florida International University's Applied Research Center (FIU-ARC) to evaluate the effectiveness of three commercially available fixatives in controlling the mobility of soil particles on soil mounds when exposed to varying wind forces. The fixatives tested included: (1) a calcium chloride solution; (2) a petroleum hydrocarbon emulsion; and 3) a synthetic organic. As an initial step, approximately 500 lbs of uncontaminated soil was obtained from the Hanford Reservation in Washington State. Soil samples were placed in an open-loop, low speed wind tunnel and exposed to wind forces ranging from 10 to 30 miles per hour (mph). Wind erosion controlling capabilities of commercially available fixatives and soil moisture were tested at a laboratory

  20. Diversity of fungi from the mound nests of Formica ulkei and adjacent non-nest soils.

    PubMed

    Duff, Lyndon B; Urichuk, Theresa M; Hodgins, Lisa N; Young, Jocelyn R; Untereiner, Wendy A

    2016-07-01

    Culture-based methods were employed to recover 3929 isolates of fungi from soils collected in May and July 2014 from mound nests of Formica ulkei and adjacent non-nest sites. The abundance, diversity, and richness of species from nest mounds exceeded those of non-mound soils, particularly in July. Communities of fungi from mounds were more similar to those from mounds than non-mounds; this was also the case for non-mound soils with the exception of one non-mound site in July. Species of Aspergillus, Paecilomyces, and Penicillium were dominant in nest soils and represented up to 81.8% of the taxa recovered. Members of the genus Aspergillus accounted for the majority of Trichocomaceae from nests and were represented almost exclusively by Aspergillus navahoensis and Aspergillus pseudodeflectus. Dominant fungi from non-mound sites included Cladosporium cladosporioides, Geomyces pannorum, and species of Acremonium, Fusarium, Penicillium, and Phoma. Although mound nests were warmer than adjacent soils, the dominance of xerotolerant Aspergillus in soils from mounds and the isolation of the majority of Trichocomaceae at 25 and 35 °C suggests that both temperature and water availability may be determinants of fungal community structure in nests of F. ulkei. PMID:27192606

  1. Deformation mechanisms adjacent to a thrust fault, Sangre de Cristo Mountains, Colorado

    SciTech Connect

    Kelly, J.C.; McConnell, D.A.; Friberg, V.M. . Dept. of Geology)

    1994-04-01

    The purpose of this study is to examine the character of grain-scale deformation adjacent to a Laramide thrust fault in the Sangre de Cristo Mountains. This site represents a window through the hanging wall of a thrust sheet which juxtaposes Precambrian rocks over Pennsylvanian rocks. It provides a rare opportunity to examine deformation mechanisms in the footwall of a basement-involved thrust. Brittle deformation is evident at both outcrop and grain-scale. Filled fractures and slickensides composed of quartz and epidote are present throughout the area, and increase in abundance adjacent to the fault zone, as does the frequency of mesoscopic faulting. Variations in deformation mechanisms can be seen between the Precambrian rocks of the thrust sheet and the Pennsylvanian metasedimentary rocks, and between the metamorphosed arkoses and metapelites within the Pennsylvanian section. Cataclastic textures are present in deformed Precambrian rocks, and the degree of cataclasis is greatest immediately adjacent to the fault zone. Deformation in the Pennsylvanian rocks is largely dependent upon the abundance of fine-grained matrix within each sample. The degree of brittle deformation is negatively correlated to the percentage of matrix. Coarser-grained sections show microscopic faults which offset quartz and feldspar grains. Offsets decrease on the faults as they pass from coarse grains into the matrix.

  2. Sites and homeostatic control of auxin biosynthesis in Arabidopsis during vegetative growth.

    PubMed

    Ljung, K; Bhalerao, R P; Sandberg, G

    2001-11-01

    The distribution and biosynthesis of indole-3-acetic acid (IAA) was investigated during early plant development in Arabidopsis. The youngest leaves analysed, less than 0.5 mm in length, contained 250 pg mg(-1) of IAA and also exhibited the highest relative capacity to synthesize this hormone. A decrease of nearly one hundred-fold in IAA content occurred as the young leaves expanded to their full size, and this was accompanied by a clear shift in both pool size and IAA synthesis capacity. The correlation between high IAA content and intense cell division was further verified in tobacco leaves, where a detailed analysis revealed that dividing mesophyll tissue contained ten-fold higher IAA levels than tissue growing solely by elongation. We demonstrated that all parts of the young Arabidopsis plant can potentially contribute to the auxin needed for growth and development, as not only young leaves, but also all other parts of the plant such as cotyledons, expanding leaves and root tissues have the capacity to synthesize IAA de novo. We also observed that naphthylphthalamic acid (NPA) treatment induced tissue-dependent feedback inhibition of IAA biosynthesis in expanding leaves and cotyledons, but intriguingly not in young leaves or in the root system. This observation supports the hypothesis that there is a sophisticated tissue-specific regulatory mechanism for auxin biosynthesis. Finally, a strict requirement for maintaining the pool sizes of IAA was revealed as reductions in leaf expansion followed both decreases and increases in the IAA levels in developing leaves. This indicates that leaves are not only important sources for IAA synthesis, but that normal leaf expansion depends on rigorous control of IAA homeostasis. PMID:11737783

  3. A genome walking strategy for the identification of nucleotide sequences adjacent to known regions.

    PubMed

    Wang, Hailong; Yao, Ting; Cai, Mei; Xiao, Xiuqing; Ding, Xuezhi; Xia, Liqiu

    2013-02-01

    To identify the transposon insertion sites in a soil actinomycete, Saccharopolyspora spinosa, a genome walking approach, termed SPTA-PCR, was developed. In SPTA-PCR, a simple procedure consisting of TA cloning and a high stringency PCR, following the single primer-mediated, randomly-primed PCR, can eliminate non-target DNA fragments and obtain target fragments specifically. Using SPTA-PCR, the DNA sequence adjacent to the highly conserved region of lectin coding gene in onion plant, Allium chinense, was also cloned. PMID:23108875

  4. Hub-mounted actuators for blade pitch collective control

    NASA Technical Reports Server (NTRS)

    Jeffery, Philip A. E. (Inventor); Luecke, Greg R. (Inventor)

    1985-01-01

    Blade collective pitch control is provided for a rotor system by rotary actuators located between adjacent blades. Each actuator is connected to the leading edge of one adjacent blade and the trailing edge of the other adjacent blade.

  5. Using the binding site to control the magnetic and spintronic properties of a single magnetic molecule in a tunnel junction

    NASA Astrophysics Data System (ADS)

    Warner, Ben; El Hallak, Fadi; Prueser, Henning; Gill, Tobias G.; Sharp, John; Fisher, Andrew J.; Persson, Mats; Hirjibehedin, Cyrus F.

    2015-03-01

    Many proposals outline the use of single magnetic molecules in new applications in information technology and spintronics, with the intention of creating new devices based on phenomena that only manifest at the atomic scale. To create these devices it will be necessary to engineer the required properties, whether through controlling the molecule's chemical makeup or its interaction with the external surroundings. The latter may involve using interactions with the supporting substrate surface, which have been shown to not only modify the molecule properties but also create effects such as chirality. Here we utilize the surface interaction to modify the properties of FePc on copper nitride, a thin insulator, above bulk Cu(001). Using scanning tunneling microscopy we show that the interaction with the surface is defined by the binding site of the central Fe atom in the molecule. By performing elastic and inelastic tunneling spectroscopy and comparing the results to DFT modeling, we explore how coupling to the surface can be used to control the molecular orbitals and the accessibility of the spin excitations. This demonstrates the importance of controlling molecule-substrate coupling down to the atomic scale for the development of single molecule devices.

  6. Insights Into Contamination Control For The Shuttle Payload Integration Facility (SPIF) At The Eastern Launch Site (ELS)

    NASA Astrophysics Data System (ADS)

    Pugel, Nancy J.

    1982-02-01

    The Shuttle Payload Integration Facility (SPIF) is a payload processing facility at the Eastern Launch Site (ELS), Cape Canaveral Air Force Station (CCAFS) which has been designed to accommodate assembly, test, and checkout operations for a number of Shuttle payloads, many of which are contamination sensitive. In general, the SPIF was designed to meet class 100,000, per Fed. Std. 209B (Reference 1), cleanliness requirements and operate as a class 100,000 clean area. However, due to the expansiveness of the building (which includes several airlocks, a transfer aisle, and integration cells), the variety of hardware to be processed through the facility (payloads, upper states, and support equipment), and the variety of operations to be performed for these hardware, many different contamination control methods must be carefully integrated and implemented to maintain an overall clean environment. In instances where the operation being performed is not generally compatible with the clean environment, uniaue and innovative contamination control methods must be developed with respect to ease of implementation as well as effectiveness of contamination control.

  7. 7. VIEW OF WATER TREATMENT PLANT, ADJACENT TO THE COAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. VIEW OF WATER TREATMENT PLANT, ADJACENT TO THE COAL CONVEYOR; IN THE DISTANCE IS THE FREQUENCY CHANGER HOUSE, WHICH IS ATTACHED TO SWITCH HOUSE NO. 1; LOOKING WEST. - Commonwealth Electric Company, Fisk Street Electrical Generating Station, 1111 West Cermak Avenue, Chicago, Cook County, IL

  8. Biogeochemistry of hydrothermally and adjacent non-altered soils

    Technology Transfer Automated Retrieval System (TEKTRAN)

    As a field/lab project, students in the Soil Biogeochemistry class of the University of Nevada, Reno described and characterized seven pedons, developed in hydrothermally and adjacent non-hydrothermally altered andesitic parent material near Reno, NV. Hydrothermally altered soils had considerably lo...

  9. 22. Float located adjacent to entry stair in filtration bed. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. Float located adjacent to entry stair in filtration bed. The float actuates a valve that maintains water level over the bed. - Lake Whitney Water Filtration Plant, Filtration Plant, South side of Armory Street between Edgehill Road & Whitney Avenue, Hamden, New Haven County, CT

  10. 2. VIEW FROM ROOFTOP OF BUILDING (MOTEL) ADJACENT TO TECHWOOD ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW FROM ROOFTOP OF BUILDING (MOTEL) ADJACENT TO TECHWOOD HOMES, LOOKING WEST. GEORGIA TECH DORMITORY BUILDING, 581-587 TECHWOOD DRIVE, IN FOREGROUND. - Techwood Homes (Public Housing), Bounded by North Avenue, Parker Street, William Street & Lovejoy Street, Atlanta, Fulton County, GA

  11. 10. Detail and contextual view of bridge and adjacent farmstead ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Detail and contextual view of bridge and adjacent farmstead setting. Note laced vertical compression members, latticed portal strut, decorative strut bracing, and lightness of diagonal and lateral tension members. View to southeast through southeast portal from truss mid-span. - Red Bank Creek Bridge, Spanning Red Bank Creek at Rawson Road, Red Bluff, Tehama County, CA

  12. LEHR NO. 2 AND LEHR NO. 3 ADJACENT TO FURNACE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    LEHR NO. 2 AND LEHR NO. 3 ADJACENT TO FURNACE ROOM; THE PIPES AT THE BOTTOM ARE PART OF THE RADIANT HEATING SYSTEM USED FOR HEATING THE FACTORY DURING COLD WEATHER. - Westmoreland Glass Company, Seventh & Kier Streets, Grapeville, Westmoreland County, PA

  13. Effects on stink bugs of field edges adjacent to woodland

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Producers face significant crop losses from stink bug species in the southeastern USA, but the high mobility and polyphagy of the bugs make predictions of their presence in crops difficult. While there is some evidence that they colonize crops from adjacent crops, there are no studies of their colo...

  14. VIEW FROM ATOP ADJACENT RESIDENTIAL TOWER, SHOWING INTERSECTION OF ACACIA ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW FROM ATOP ADJACENT RESIDENTIAL TOWER, SHOWING INTERSECTION OF ACACIA ROAD WITH BIRCH CIRCLE. VIEW FACING NORTHEAST - Camp H.M. Smith and Navy Public Works Center Manana Title VII (Capehart) Housing, Intersection of Acacia Road and Brich Circle, Pearl City, Honolulu County, HI

  15. VIEW FROM ATOP ADJACENT RESIDENTIAL TOWER, SHOWING RECREATION AREA AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW FROM ATOP ADJACENT RESIDENTIAL TOWER, SHOWING RECREATION AREA AND ENTRY TO NEIGHBORHOOD. VIEW FACING SOUTHEAST - Camp H.M. Smith and Navy Public Works Center Manana Title VII (Capehart) Housing, Intersection of Acacia Road and Brich Circle, Pearl City, Honolulu County, HI

  16. VIEW FROM ATOP ADJACENT RESIDENTIAL TOWER, SHOWING WESTERN SIDE OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW FROM ATOP ADJACENT RESIDENTIAL TOWER, SHOWING WESTERN SIDE OF NEIGHBORHOOD. VIEW FACING NORTHWEST - Camp H.M. Smith and Navy Public Works Center Manana Title VII (Capehart) Housing, Intersection of Acacia Road and Brich Circle, Pearl City, Honolulu County, HI

  17. VIEW FROM ATOP ADJACENT RESIDENTIAL TOWER, SHOWING RECREATION AREA ON ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW FROM ATOP ADJACENT RESIDENTIAL TOWER, SHOWING RECREATION AREA ON RIGHT, AND HOUSING AREA ON LEFT. VIEW FACING EAST/NORTHEAST - Camp H.M. Smith and Navy Public Works Center Manana Title VII (Capehart) Housing, Intersection of Acacia Road and Brich Circle, Pearl City, Honolulu County, HI

  18. How subaerial salt extrusions influence water quality in adjacent aquifers

    NASA Astrophysics Data System (ADS)

    Mehdizadeh, Razieh; Zarei, Mehdi; Raeisi, Ezzat

    2015-12-01

    Brines supplied from salt extrusions cause significant groundwater salinization in arid and semi-arid regions where salt rock is exposed to dissolution by episodic rainfalls. Here we focus on 62 of the 122 diapirs of Hormuz salt emergent in the southern Iran. To consider managing the degradation effect that salt extrusions have on the quality of adjoining aquifers, it is first necessary to understand how they influence adjacent water resources. We evaluate here the impacts that these diapirs have on adjacent aquifers based on investigating their geomorphologies, geologies, hydrologies and hydrogeologies. The results indicate that 28/62 (45%) of our sample of salt diapirs have no significant impact on the quality of groundwater in adjoining aquifers (namely Type N), while the remaining 34/62 (55%) degrade nearby groundwater quality. We offer simple conceptual models that account for how brines flowing from each of these types of salt extrusions contaminate adjacent aquifers. We identify three main mechanisms that lead to contamination: surface impact (Type A), subsurface intrusion (Type B) and indirect infiltration (Type C). A combination of all these mechanisms degrades the water quality in nearby aquifers in 19/62 (31%) of the salt diapirs studied. Having characterized the mechanism(s) by which each diapir affects the adjacent aquifer, we suggest a few possible remediation strategies to be considered. For instance, engineering the surface runoff of diapirs Types A and C into nearby evaporation basins would improve groundwater quality.

  19. 45. 1915 CLOTH ROOM ADJACENT TO PICKER ROOM, SECOND FLOOR, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    45. 1915 CLOTH ROOM ADJACENT TO PICKER ROOM, SECOND FLOOR, NORTH END OF MILL NO. 2, WALL ON LEFT DIVIDING CLOTH ROOM ADDED LATER (PROBABLY C. 1970s). - Prattville Manufacturing Company, Number One, 242 South Court Street, Prattville, Autauga County, AL

  20. Detail of north intermediate abutment pylon showing proximity of adjacent ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of north intermediate abutment pylon showing proximity of adjacent 1001-1007 East First Street (James K. Hill and Sons Pickle Works Building), facing east - First Street Bridge, Spanning Los Angeles River at First Street, Los Angeles, Los Angeles County, CA

  1. 8. Exterior view, showing tank and associated piping adjacent to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Exterior view, showing tank and associated piping adjacent to Test Cell 6, Systems Integration Laboratory Building (T-28), looking south. - Air Force Plant PJKS, Systems Integration Laboratory, Systems Integration Laboratory Building, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO

  2. 4. REAR ELEVATION, DETAIL OF CONSTRUCTION, ADJACENT CORNER POSTS BETWEEN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. REAR ELEVATION, DETAIL OF CONSTRUCTION, ADJACENT CORNER POSTS BETWEEN BUILDING PERIODS 1 AND 3. NOTE REUSED WOOD STRIP NAILED TO BUILDING PERIOD 1 POST INSCRIBED 'ST. LEONARD'. THERE ARE NO NAIL HOLES IN THE PERIOD 3 POST, THE FARRING STRIPS ADJUST FOR CLADDING - Charles' Gift, State Routes 2 & 4, Lusby, Calvert County, MD

  3. 4. Elevation looking southwest from adjacent hills on northeast side ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Elevation looking southwest from adjacent hills on northeast side of bridge, taken from river level. Note entire east side and substructure. - Presumpscot Falls Bridge, Spanning Presumptscot River at Allen Avenue extension, 0.75 mile west of U.S. Interstate 95, Falmouth, Cumberland County, ME

  4. 12. LOG FOUNDATION ELEMENTS OF THE SAWMILL ADJACENT TO THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. LOG FOUNDATION ELEMENTS OF THE SAWMILL ADJACENT TO THE CANAL, LOOKING EAST. BARREN AREA IN FOREGROUND IS DECOMPOSING SAWDUST. DIRT PILE IN BACKGROUND IS THE EDGE OF THE SUMMIT COUNTY LANDFILL. - Snake River Ditch, Headgate on north bank of Snake River, Dillon, Summit County, CO

  5. Split-thickness skin graft donor site management: a randomized controlled trial comparing polyurethane with calcium alginate dressings.

    PubMed

    Higgins, Louise; Wasiak, Jason; Spinks, Anneliese; Cleland, Heather

    2012-04-01

    Split-thickness skin grafting (SSG) is a common reconstructive technique for the treatment of patients with deep burns and other traumatic injuries. The management of the donor site after harvesting an SSG remains controversial because of a variety of dressings available for use. The aim of this randomized controlled trial was to compare the effectiveness of a polyurethane dressing, Allevyn™, to a calcium alginate, Kaltostat®. From August 2009 to April 2010, 36 patients were randomized to Allevyn™ or Kaltostat® for donor site management following split skin graft surgery. Pain intensity and adverse events were the primary outcomes assessed. Secondary outcome measures included time for wound healing, ease of application and removal and overall patient satisfaction. Time to first dressing change was earlier in those randomized to Allevyn™ compared with Kaltostat® (5·5 days versus 8·11 days, P = 0·014). In patients randomized to Allevyn™, excessive exudate lead to a significantly increased number of dressing changes before day 10 (14 days versus 7 days, P = 0·018). The total number of dressing changes applied was also greater in those with Allevyn™ compared with Kaltstat® (P = 0·007). There were no significant differences between the two treatment groups with respect to time to wound healing, level of pain intensity, length of stay, staff and patient satisfaction levels. This trial showed Allevyn™ to be associated with increase demands on nursing time, increased cost of dressing products, medical consumables and wastes. Kaltostat® remains the dressing of choice for initial donor site dressing in this burns unit. PMID:22051247

  6. Impact of Co-Site Interference on L/C-Band Spectrum for UAS Control and Non-Payload Communications

    NASA Technical Reports Server (NTRS)

    Kerczewski, Robert J.; Bishop, William D.; Hoder, Douglas J.; Shalkhauser, Kurt A.; Wilson, Jeffrey D.

    2015-01-01

    In order to provide for the safe integration of unmanned aircraft systems into the National Airspace System, the control and non-payload communications (CNPC) link connecting the ground-based pilot with the unmanned aircraft must be highly reliable. A specific requirement is that it must operate using aviation safety radiofrequency spectrum. The 2012 World Radiocommunication Conference (WRC-12) provided a potentially suitable allocation for LOS CNPC spectrum in C-Band at 5030-5091 MHz band which, when combined with a previous allocation in L-Band (960-1164 MHz) may satisfy the LOS spectrum requirement and provide for high reliability through dual-band redundancy. However, the LBand spectrum hosts a number of aeronautical navigation systems which require high-power transmitters on-board the aircraft. These high-power transmitters co-located with sensitive CNPC receivers operating in the same frequency band have the potential to create co-site interference, reducing the performance of the CNPC receivers and ultimately reducing the usability of the L-Band for CNPC. This paper examines the potential for co-site interference, as highlighted in recent flight tests, and discusses the impact on the UAS CNPC spectrum availability and requirements for further testing and analysis.

  7. Nucleosome positioning and kinetics near transcription-start-site barriers are controlled by interplay between active remodeling and DNA sequence

    PubMed Central

    Parmar, Jyotsana J.; Marko, John F.; Padinhateeri, Ranjith

    2014-01-01

    We investigate how DNA sequence, ATP-dependent chromatin remodeling and nucleosome-depleted ‘barriers’ co-operate to determine the kinetics of nucleosome organization, in a stochastic model of nucleosome positioning and dynamics. We find that ‘statistical’ positioning of nucleosomes against ‘barriers’, hypothesized to control chromatin structure near transcription start sites, requires active remodeling and therefore cannot be described using equilibrium statistical mechanics. We show that, unlike steady-state occupancy, DNA site exposure kinetics near a barrier is dominated by DNA sequence rather than by proximity to the barrier itself. The timescale for formation of positioning patterns near barriers is proportional to the timescale for active nucleosome eviction. We also show that there are strong gene-to-gene variations in nucleosome positioning near barriers, which are eliminated by averaging over many genes. Our results suggest that measurement of nucleosome kinetics can reveal information about sequence-dependent regulation that is not apparent in steady-state nucleosome occupancy. PMID:24068556

  8. Subsidence in geopressured geothermal resource test sites: Monitoring assessment combining geodetic leveling and tidal control stations in southwestern Louisiana

    SciTech Connect

    Ramsey, K.E.; John, C.J. ); Trahan, D.B. )

    1989-09-01

    The Louisiana Geological Survey has an ongoing environmental monitoring program, sponsored by the US Department of Energy, at geopressured geothermal prospect well sites in southwestern Louisiana. This paper presents the results from monitoring subsidence at some of these reservoir sites. Over 1,000 km of first-order surveys and data from several NOAA and US Army Corps of Engineers tidal control stations were examined to determine regional trends. Tidal records were used to examine the history of sea level with respect to the land surface. Relative rates of land subsidence can be determined by comparing rates of water level rise over time with rates of rise from a stable craton. Regional subsidence ranges from 3 to 5 mm/year. First-order bench-mark networks established at Parcperdue, Sweet Lake, and Gladys McCall prospects were used to determine local trends of subsidence. Repeated leveling surveys before, during, and after fluid withdrawal from Parcperdue and Gladys McCall indicate that an increase in subsidence was observed during the drilling of the wells. Data suggest subsidence was possibly due to surface loading by heavy drilling equipment. Historical leveling in the Sweet Lake region indicates differential compaction between sediments as a possible cause for subsidence. However, in all cases, virtually no increase in subsidence was observed during and after times of fluid withdrawal.

  9. Case histories of electrical resistivity and controlled-source magnetotelluric surveys for the site investigation of tunnel construction

    SciTech Connect

    Kwon, H.S.; Song, Y.; Yi, M.J.; Chung, H.J.; Kim, K.S.

    2006-12-15

    In tunnel construction, the information regarding rock mass quality and the distribution of weak zones is crucial for economical tunnel design and to ensure safety. Usually, the rock mass grade is estimated by observing recovered cores obtained by drilling or by physical parameters calculated in a laboratory using core samples. However, the high drilling cost limits the number of boreholes; furthermore, rough terrains can reduce the access of drilling machines to the survey sites. In such situations, surface geophysical methods such as electrical resistivity or controlled-source magnetotelluric (CSMT) can provide a rough estimate of the rock mass condition over the planned tunnel route. These methods can also map weak zones (faults, fractures, coal bearing zones, and cavities), which are characterized by a lower resistivity than the surrounding fresh rock mass. We present two successful applications of the electrical resistivity and CSMT methods to the site investigation of tunnel construction over a rough terrain. The first example demonstrates that the boundary of the bedrock and weak zones related to the distribution of coaly shale and coal seams were estimated to extend beyond a few hundred meters below the rough surface. The second example shows that the developing direction and depth of cavities, which are mainly related to the weak zones in limestone, were successfully interpreted by a three-dimensional (3-D) electrical resistivity survey with the aid of borehole test results.

  10. Pollution prevention opportunity assessment for the K-25 Site Toxic Substances Control Act Incinerator Operations, Level III

    SciTech Connect

    1995-09-01

    A Level III pollution prevention opportunity assessment (PPOA) was performed for the Oak Ridge K-25 Site Toxic Substances Control Act (TSCA) Incinerator to evaluate pollution prevention (P2) options for various waste streams: The main objective of this study was to identify and evaluate options to reduce the quantities of each waste stream generated by the TSCA Incinerator operations to realize significant environmental and/or economic benefits from P2. For each of the waste streams, P2 options were evaluated following the US Environmental Protection Agency (EPA) hierarchy to (1) reduce the quantity of waste generated, (2) recycle the waste, and/or (3) use alternate waste treatment or segregation methods. This report provides process descriptions, identification and evaluation of P2 options, and final recommendations.

  11. Descriptive analysis and spatial epidemiology of porcine reproductive and respiratory syndrome (PRRS) for swine sites participating in area regional control and elimination programs from 3 regions of Ontario

    PubMed Central

    Arruda, Andreia G.; Poljak, Zvonimir; Friendship, Robert; Carpenter, Jane; Hand, Karen

    2015-01-01

    The objectives of this study were to describe demographics, basic biosecurity practices, ownership structure, and prevalence of porcine reproductive and respiratory syndrome (PRRS) in swine sites located in 3 regions in Ontario, and investigate the presence of spatial clustering and clusters of PRRS positive sites in the 3 regions. A total of 370 swine sites were enrolled in Area Regional Control and Elimination projects in Niagara, Watford, and Perth from 2010 to 2013. Demographics, biosecurity, and site ownership data were collected using a standardized questionnaire and site locations were obtained from an industry organization. Status was assigned on the basis of available diagnostic tests and/or assessment by site veterinarians. Spatial dependence was investigated using the D-function, the spatial scan statistic test and the spatial relative risk method. Results showed that the use of strict all-in all-out (AIAO) pig flow and shower before entry are uncommon biosecurity practices in swine sites, but a larger proportion of sites reported having a Danish entry. The prevalence of PRRS in the 3 regions ranged from 17% to 48% and localized high and low risk clusters were detected. Sites enrolled in the PRRS control projects were characterized by membership in multiple and overlapping ownership structures and networks, which complicates the way the results of monitoring and disease management measures are communicated to the target population. PMID:26424906

  12. Descriptive analysis and spatial epidemiology of porcine reproductive and respiratory syndrome (PRRS) for swine sites participating in area regional control and elimination programs from 3 regions of Ontario.

    PubMed

    Arruda, Andreia G; Poljak, Zvonimir; Friendship, Robert; Carpenter, Jane; Hand, Karen

    2015-10-01

    The objectives of this study were to describe demographics, basic biosecurity practices, ownership structure, and prevalence of porcine reproductive and respiratory syndrome (PRRS) in swine sites located in 3 regions in Ontario, and investigate the presence of spatial clustering and clusters of PRRS positive sites in the 3 regions. A total of 370 swine sites were enrolled in Area Regional Control and Elimination projects in Niagara, Watford, and Perth from 2010 to 2013. Demographics, biosecurity, and site ownership data were collected using a standardized questionnaire and site locations were obtained from an industry organization. Status was assigned on the basis of available diagnostic tests and/or assessment by site veterinarians. Spatial dependence was investigated using the D-function, the spatial scan statistic test and the spatial relative risk method. Results showed that the use of strict all-in all-out (AIAO) pig flow and shower before entry are uncommon biosecurity practices in swine sites, but a larger proportion of sites reported having a Danish entry. The prevalence of PRRS in the 3 regions ranged from 17% to 48% and localized high and low risk clusters were detected. Sites enrolled in the PRRS control projects were characterized by membership in multiple and overlapping ownership structures and networks, which complicates the way the results of monitoring and disease management measures are communicated to the target population. PMID:26424906

  13. Respiratory hospitalizations of children living near a hazardous industrial site adjusted for prevalent dust: a case-control study.

    PubMed

    Nirel, Ronit; Maimon, Nimrod; Fireman, Elizabeth; Agami, Sarit; Eyal, Arnona; Peretz, Alon

    2015-03-01

    The Neot Hovav Industrial Park (IP), located in southern Israel, hosts 23 chemical industry facilities and the national site for treatment of hazardous waste. Yet, information about its impact on the health of local population has been mostly ecological, focused on Bedouins and did not control for possible confounding effect of prevalent dust storms. This case-control study examined whether living near the IP could lead to increased risk of pediatric hospitalization for respiratory diseases. Cases (n=3608) were residents of the Be'er Sheva sub-district aged 0-14 years who were admitted for respiratory illnesses between 2004 and 2009. These were compared to children admitted for non-respiratory conditions (n=3058). Home addresses were geocoded and the distances from the IP to the child's residence were calculated. The association between hospitalization and residential distance from the IP was examined for three age groups (0-1, 2-6, 7-14) by logistic regressions adjusting for gender, socioeconomic status, urbanity and temperature. We found that infants in the first year of life who lived within 10 km of the IP had increased risk of respiratory hospitalization when compared with those living >20 km from the IP (adjusted odds ratio, OR=2.07, 95% confidence interval, CI: 1.19-3.59). In models with both distance from the IP and particulate matter with aerodynamic diameter <10 μm (PM(10)) the estimated risk was modestly attenuated (OR=1.96, 95% CI: 1.09-3.51). Elevated risk was also observed for children 2-5 years of age but with no statistical significance (OR=1.16, 95% CI: 0.76-1.76). Our findings suggest that residential proximity to a hazardous industrial site may contribute to early life respiratory admissions, beyond that of prevailing PM(10). PMID:25547415

  14. Site-specific mesenchymal control of inflammatory pain to yeast challenge in vulvodynia afflicted and pain-free women

    PubMed Central

    Foster, David C.; Falsetta, Megan L.; Woeller, Collynn F.; Pollock, Stephen J.; Song, Kunchang; Bonham, Adrienne; Haidaris, Constantine G.; Stodgell, Chris J.; Messing, Susan P.; Iadarola, Michael; Phipps, Richard P.

    2015-01-01

    Fibroblast strains were derived from two regions of the lower genital tract of localized provoked vulvodynia (LPV) cases and pain-free controls. Sixteen strains were derived from four cases and four controls, age and race matched, following pre-sampling mechanical pain threshold assessments. Strains were challenged with six separate stimuli: live yeast species (C. albicans, C. glabrata, C. tropicalis, and S. cerevisiae), yeast extract (zymosan), or inactive vehicle. Production of prostaglandin E2 (PGE2) and interleukin-6 (IL-6) were pro-inflammatory response measures. Highest IL-6 and PGE2 occurred with vestibular strains following C. albicans, C. glabrata, and zymosan challenges, resulting in the ability to significantly predict IL-6 and PGE2 production by genital tract location. Following C. albicans and C. glabrata challenge of all sixteen fibroblast strains, adjusting for dual sampling of subjects, PGE2 and IL-6 production significantly predicted the pre-sampling pain threshold from the genital tract site of sampling. At the same location of pain assessment and fibroblast sampling, in situ immunohistochemical (IHC)(+) fibroblasts for IL-6 and Cox-2 were quantified microscopically. The correlation between IL-6 production and IL-6 IHC(+) was statistically significant yet biological significance is unknown because of the small number of IHC(+) IL-6 fibroblasts identified. A low fibroblast IL-6 IHC(+) count may result from most IL-6 produced by fibroblasts existing in a secreted, extracellular state. Enhanced, site-specific, innate immune responsiveness to yeast pathogens by fibroblasts may be an early step in LPV pathogenesis. Fibroblast strain testing may offer an attractive/objective marker of LPV pathology in women with vulvodynia of inflammatory origin. PMID:25679469

  15. Terrigenous Sedimentation Patterns at Reefs Adjacent to the Guanica Bay Watershed, Southwest Puerto Rico

    NASA Astrophysics Data System (ADS)

    Sherman, C.; Whitall, D.

    2014-12-01

    Guanica Bay is an estuary on the southwest coast of Puerto Rico with numerous nearshore reefs located in adjacent coastal waters. As part of the multi-agency Guanica Bay Watershed Project, a study was undertaken to establish baseline levels of terrigenous sedimentation reaching reefs adjacent to the Guanica Bay watershed as well as establish spatial and temporal patterns in its delivery. To characterize and quantify sedimentation patterns, sediment traps were established at nine reef sites occurring along an ~ 14 km stretch of coastline centered on the outlet of the bay. Sites were located at shallow reefs within 2 km of the shore at depths of ~ 10 m. Two additional sites were located at the mouth of the Rio Loco where it empties into Guanica Bay and at the mouth of the bay where it opens into adjacent coastal waters. Traps were collected monthly from August 2009 through July 2012 to determine both the amount of sediment accumulation (mg cm-2 day-1) and its composition. Composition is expressed in terms of relative amounts of calcium carbonate (in situ production), organic material and terrigenous material. Average trap accumulation rates among the reef sites ranged from ~ 3 to 28 mg cm-2 day-1. Average percent terrigenous material within reef accumulation ranged from ~ 20% to 30%. While trap accumulation rates are highly variable on both spatial and temporal scales, the composition of sediments and relative amount of terrigenous material is fairly uniform. Similar temporal patterns in accumulation rates among the sites without corresponding changes in composition of sediments point to resuspension of bottom sediments by wave action as a primary driver of sedimentary dynamics at these reefs. Sites closest to Guanica Bay display the highest degree of terrigenous influence in terms of trap accumulation rates and percent terrigenous material, which is consistent with Guanica Bay serving as a local source of terrigenous material to coastal waters. However, the lack of

  16. Agricultural Intensification Exacerbates Spillover Effects on Soil Biogeochemistry in Adjacent Forest Remnants

    PubMed Central

    Didham, Raphael K.; Barker, Gary M.; Bartlam, Scott; Deakin, Elizabeth L.; Denmead, Lisa H.; Fisk, Louise M.; Peters, Jennifer M. R.; Tylianakis, Jason M.; Wright, Hannah R.; Schipper, Louis A.

    2015-01-01

    Land-use intensification is a central element in proposed strategies to address global food security. One rationale for accepting the negative consequences of land-use intensification for farmland biodiversity is that it could ‘spare’ further expansion of agriculture into remaining natural habitats. However, in many regions of the world the only natural habitats that can be spared are fragments within landscapes dominated by agriculture. Therefore, land-sparing arguments hinge on land-use intensification having low spillover effects into adjacent protected areas, otherwise net conservation gains will diminish with increasing intensification. We test, for the first time, whether the degree of spillover from farmland into adjacent natural habitats scales in magnitude with increasing land-use intensity. We identified a continuous land-use intensity gradient across pastoral farming systems in New Zealand (based on 13 components of farmer input and soil biogeochemistry variables), and measured cumulative off-site spillover effects of fertilisers and livestock on soil biogeochemistry in 21 adjacent forest remnants. Ten of 11 measured soil properties differed significantly between remnants and intact-forest reference sites, for both fenced and unfenced remnants, at both edge and interior. For seven variables, the magnitude of effects scaled significantly with magnitude of surrounding land-use intensity, through complex interactions with fencing and edge effects. In particular, total C, total N, δ15N, total P and heavy-metal contaminants of phosphate fertilizers (Cd and U) increased significantly within remnants in response to increasing land-use intensity, and these effects were exacerbated in unfenced relative to fenced remnants. This suggests movement of livestock into surrounding natural habitats is a significant component of agricultural spillover, but pervasive changes in soil biogeochemistry still occur through nutrient spillover channels alone, even in fenced

  17. Child sexual abuse prevention training for childcare professionals: an independent multi-site randomized controlled trial of Stewards of Children.

    PubMed

    Rheingold, Alyssa A; Zajac, Kristyn; Chapman, Jason E; Patton, Meghan; de Arellano, Michael; Saunders, Benjamin; Kilpatrick, Dean

    2015-04-01

    Given the significant rates and deleterious consequences of childhood sexual abuse (CSA), identifying effective primary prevention approaches is a clear priority. There isa growing awareness that childcare professionals (e.g.,teachers, childcare personnel, clergy) are in a unique position to engage in prevention efforts due to high accessibility to children and expertise in child development. However, CSA prevention programs targeting childcare professionals have received insufficient attention. The goal of this study was toc on duct an independent multi-site controlled evaluation of an existing CSA prevention program, Stewards of Children, offered through both in-person and web-based formats. This study included 352 childcare professionals recruited from children’s advocacy centers across three states. Participants were randomly assigned to one of three conditions: (1) inperson training, (2) web-based training, or (3) waitlist control. Dependent variables included CSA knowledge, CSA attitudes,and self-reported CSA preventive behaviors. Results indicated that Stewards impacted knowledge, attitudes, and preventive behaviors. No differences were found between training modalities (i.e., in-person versus web-based) on knowledge and preventive behaviors. Results indicate that brief trainings for childcare professionals may impact CSA prevention efforts. PMID:25015782

  18. Catchment controls on soil moisture dynamics: from site-specific hysteresis in event responses to temporal stability of patterns

    NASA Astrophysics Data System (ADS)

    Hassler, Sibylle K.; Weiler, Markus; Blume, Theresa

    2015-04-01

    Understanding soil moisture dynamics is a prerequisite for predicting hydrological response at the hillslope and catchment scale. Soil moisture is not only determined by its input characteristics such as rainfall, its redistribution by vegetation and evapotranspiration. Catchment characteristics resulting from the interplay of geology, topography, land cover and associated soil hydraulic properties also affect the distribution, storage and transport of water in the vadose zone. Successful process predictions and appropriate hydrological model structures thus rely on a good representation of soil moisture patterns and dynamics and benefit from insights into their dependence on catchment characteristics. In a unique measurement setup at the CAOS hydrological observatory in Luxemburg (http://www.caos-project.de) we record hydro-meteorological variables at 45 sensor cluster sites. These sites are distributed across the mesoscale Attert catchment and cover three different geological units (schist, marls and sandstone), two types of land use (forest and grassland), different topographical positions (up- and downslope with north- and south-facing aspects as well as plateau and floodplain locations). At each sensor cluster, each covering approximately an area of 30 m², soil moisture is measured in three profiles at three different depths, in piezometers groundwater levels are recorded, and rain gauges collect throughfall or gross precipitation. At near-stream locations we also measure stream water levels. This extensive sensor network enables us to study the influence of geology, land use and topography on soil moisture dynamics. In this study we focus on short-term hysteretic responses related to individual rainfall events and on longer-term temporal stability of soil moisture patterns. Similarities in the hysteresis loops of rainfall/soil moisture, soil moisture/groundwater levels and soil moisture/stream water levels can give some indication of the dominant catchment

  19. Toxicity and bioavailability of metals in the Missouri River adjacent to a lead refinery

    USGS Publications Warehouse

    Chapman, Duane C.; Allert, Ann L.; Fairchild, James F.; May, Thomas W.; Schmitt, Christopher J.; Callahan, Edward V.

    2001-01-01

    This study is an evaluation of the potential environmental impacts of contaminated groundwater from the ASARCO metals refining facility adjacent to the Missouri River in Omaha, Nebraska. Surface waters, sediments, and sediment pore waters were collected from the Burt-Izard drain, which transects the facility, and from the Missouri River adjacent to the facility. Groundwater was also collected from the facility. Waters and sediments were analyzed for inorganic contaminants, and the toxicity of the waters was evaluated with the Ceriodaphnia dubia 7-day test. Concentrations of several elemental contaminants were highly elevated in the groundwater, but not in river sediment pore waters. Lead concentrations were moderately elevated in whole sediment at one site, but lead concentrations in pore waters were low due to apparent sequestration by acid-volatile sulfides. The groundwater sample was highly toxic to C. dubia, causing 100% mortality. Even at the lowest groundwater concentration tested (6.25%) C. dubia survival was reduced; however, at that concentration, reproduction was not significantly different from upstream porewater reference samples. Sediment pore waters were not toxic, except reproduction in pore water collected from one downstream site was somewhat reduced. The decrease in reproduction could not be attributed to measured elemental contaminants.

  20. Malaria Transmission, Infection, and Disease at Three Sites with Varied Transmission Intensity in Uganda: Implications for Malaria Control

    PubMed Central

    Kamya, Moses R.; Arinaitwe, Emmanuel; Wanzira, Humphrey; Katureebe, Agaba; Barusya, Chris; Kigozi, Simon P.; Kilama, Maxwell; Tatem, Andrew J.; Rosenthal, Philip J.; Drakeley, Chris; Lindsay, Steve W.; Staedke, Sarah G.; Smith, David L.; Greenhouse, Bryan; Dorsey, Grant

    2015-01-01

    The intensification of control interventions has led to marked reductions in malaria burden in some settings, but not others. To provide a comprehensive description of malaria epidemiology in Uganda, we conducted surveillance studies over 24 months in 100 houses randomly selected from each of three subcounties: Walukuba (peri-urban), Kihihi (rural), and Nagongera (rural). Annual entomological inoculation rate (aEIR) was estimated from monthly Centers for Disease Control and Prevention (CDC) light trap mosquito collections. Children aged 0.5–10 years were provided long-lasting insecticidal nets (LLINs) and followed for measures of parasite prevalence, anemia and malaria incidence. Estimates of aEIR were 2.8, 32.0, and 310 infectious bites per year, and estimates of parasite prevalence 7.4%, 9.3%, and 28.7% for Walukuba, Kihihi, and Nagongera, respectively. Over the 2-year study, malaria incidence per person-years decreased in Walukuba (0.51 versus 0.31, P = 0.001) and increased in Kihihi (0.97 versus 1.93, P < 0.001) and Nagongera (2.33 versus 3.30, P < 0.001). Of 2,582 episodes of malaria, only 8 (0.3%) met criteria for severe disease. The prevalence of anemia was low and not associated with transmission intensity. In our cohorts, where LLINs and prompt effective treatment were provided, the risk of complicated malaria and anemia was extremely low. However, malaria incidence was high and increased over time at the two rural sites, suggesting improved community-wide coverage of LLIN and additional malaria control interventions are needed in Uganda. PMID:25778501

  1. Malaria transmission, infection, and disease at three sites with varied transmission intensity in Uganda: implications for malaria control.

    PubMed

    Kamya, Moses R; Arinaitwe, Emmanuel; Wanzira, Humphrey; Katureebe, Agaba; Barusya, Chris; Kigozi, Simon P; Kilama, Maxwell; Tatem, Andrew J; Rosenthal, Philip J; Drakeley, Chris; Lindsay, Steve W; Staedke, Sarah G; Smith, David L; Greenhouse, Bryan; Dorsey, Grant

    2015-05-01

    The intensification of control interventions has led to marked reductions in malaria burden in some settings, but not others. To provide a comprehensive description of malaria epidemiology in Uganda, we conducted surveillance studies over 24 months in 100 houses randomly selected from each of three subcounties: Walukuba (peri-urban), Kihihi (rural), and Nagongera (rural). Annual entomological inoculation rate (aEIR) was estimated from monthly Centers for Disease Control and Prevention (CDC) light trap mosquito collections. Children aged 0.5-10 years were provided long-lasting insecticidal nets (LLINs) and followed for measures of parasite prevalence, anemia and malaria incidence. Estimates of aEIR were 2.8, 32.0, and 310 infectious bites per year, and estimates of parasite prevalence 7.4%, 9.3%, and 28.7% for Walukuba, Kihihi, and Nagongera, respectively. Over the 2-year study, malaria incidence per person-years decreased in Walukuba (0.51 versus 0.31, P = 0.001) and increased in Kihihi (0.97 versus 1.93, P < 0.001) and Nagongera (2.33 versus 3.30, P < 0.001). Of 2,582 episodes of malaria, only 8 (0.3%) met criteria for severe disease. The prevalence of anemia was low and not associated with transmission intensity. In our cohorts, where LLINs and prompt effective treatment were provided, the risk of complicated malaria and anemia was extremely low. However, malaria incidence was high and increased over time at the two rural sites, suggesting improved community-wide coverage of LLIN and additional malaria control interventions are needed in Uganda. PMID:25778501

  2. Hydraulic and nutritional feedback controls surface patchiness of biological soil crusts at a post-mining site.

    NASA Astrophysics Data System (ADS)

    Fischer, Thomas; Gypser, Stella; Subbotina, Maria; Veste, Maik

    2015-04-01

    , and decreased to BSC2, BSC1 and BSC3. Non-metric multidimensional scaling revealed that the lichens and BSC3 were associated with water soluble nutrients (NO3, NH4, K, Mg, Ca) and with pyrite weathering products (pH, SO4), thus representing a high nutrient low hydraulic feedback mode. The mosses and BSC2 represented a low nutrient high hydraulic feedback mode. These feedback mechanisms were considered as synergic, consisting of run-off generating (low hydraulic) and run-on receiving (high hydraulic) BSC patches. Three scenarios for BSC succession were proposed. (1) Initial BSCs sealed the surface until they reached a successional stage (represented by BSC1) from which the development into either of the feedback modes was triggered, (2) initial heterogeneities of the mineral substrate controlled the development of the feedback mode, and (3) complex interactions between lichens and mosses occurred at later stages of system development. It was concluded that, irrespective of successional pathways, two synergic feedback mechanisms contributed to the generation of self-organized surface patchiness. Such small-scale microsite differentiation with different BSCs has important implications for the vegetation in post-mining sites. Reference Fischer, T., Gypser, S., Subbotina, M., Veste, M. (2014) Synergic hydraulic and nutritional feedback mechanisms control surface patchiness of biological soil crusts on tertiary sands at a post-mining site. Journal of Hydrology and Hydromechanics 62(4):293-302

  3. Differential transcriptional profiling of damaged and intact adjacent dorsal root ganglia neurons in neuropathic pain.

    PubMed

    Reinhold, A K; Batti, L; Bilbao, D; Buness, A; Rittner, H L; Heppenstall, P A

    2015-01-01

    Neuropathic pain, caused by a lesion in the somatosensory system, is a severely impairing mostly chronic disease. While its underlying molecular mechanisms are not thoroughly understood, neuroimmune interactions as well as changes in the pain pathway such as sensitization of nociceptors have been implicated. It has been shown that not only are different cell types involved in generation and maintenance of neuropathic pain, like neurons, immune and glial cells, but, also, intact adjacent neurons are relevant to the process. Here, we describe an experimental approach to discriminate damaged from intact adjacent neurons in the same dorsal root ganglion (DRG) using differential fluorescent neuronal labelling and fluorescence-activated cell sorting (FACS). Two fluorescent tracers, Fluoroemerald (FE) and 1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate (DiI), were used, whose properties allow us to distinguish between damaged and intact neurons. Subsequent sorting permitted transcriptional analysis of both groups. Results and qPCR validation show a strong regulation in damaged neurons versus contralateral controls as well as a moderate regulation in adjacent neurons. Data for damaged neurons reveal an mRNA expression pattern consistent with established upregulated genes like galanin, which supports our approach. Moreover, novel genes were found strongly regulated such as corticotropin-releasing hormone (CRH), providing novel targets for further research. Differential fluorescent neuronal labelling and sorting allows for a clear distinction between primarily damaged neuropathic neurons and "bystanders," thereby facilitating a more detailed understanding of their respective roles in neuropathic processes in the DRG. PMID:25880204

  4. Influence of native flowering plant strips on natural enemies and herbivores in adjacent blueberry fields.

    PubMed

    Walton, Nathaniel J; Isaacs, Rufus

    2011-06-01

    Conservation plantings of native wildflowers were established adjacent to highbush blueberry (Vaccinium corymbosum L.) fields to test the hypothesis that provision of resources for natural enemies increases their abundance in adjacent crop fields without increasing the abundance of pest insects. For two growing seasons, natural enemies and herbivorous insects were sampled in fields with flowering borders and in control fields where growers maintained standard mown grass perimeters. Insects were categorized according to their trophic level and their potential pest status, and their abundance was compared between years and between treatments. Syrphid flies (Diptera: Syrphidae) were significantly more abundant in fields with conservation strips, as were plant bugs (Hemiptera: Miridae), thrips (Thysanoptera: Thripidae), and hoppers (Hemiptera: Auchenorrhyncha). Aphids (Hemiptera: Aphididae), thrips, fruit flies (Diptera: Tephritidae), and pirate bugs (Hemiptera: Anthocoridae) decreased significantly in abundance from 2007 to 2008. Beneficial insect abundance in crop fields increased in the latter half of the season in both years and this increase was more pronounced in fields adjacent to conservation plantings. We discuss the implications of these findings for pest management and conservation of biodiversity in farmland. PMID:22251649

  5. Differential Transcriptional Profiling of Damaged and Intact Adjacent Dorsal Root Ganglia Neurons in Neuropathic Pain

    PubMed Central

    Reinhold, A. K.; Batti, L.; Bilbao, D.; Buness, A.; Rittner, H. L.; Heppenstall, P. A.

    2015-01-01

    Neuropathic pain, caused by a lesion in the somatosensory system, is a severely impairing mostly chronic disease. While its underlying molecular mechanisms are not thoroughly understood, neuroimmune interactions as well as changes in the pain pathway such as sensitization of nociceptors have been implicated. It has been shown that not only are different cell types involved in generation and maintenance of neuropathic pain, like neurons, immune and glial cells, but, also, intact adjacent neurons are relevant to the process. Here, we describe an experimental approach to discriminate damaged from intact adjacent neurons in the same dorsal root ganglion (DRG) using differential fluorescent neuronal labelling and fluorescence-activated cell sorting (FACS). Two fluorescent tracers, Fluoroemerald (FE) and 1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate (DiI), were used, whose properties allow us to distinguish between damaged and intact neurons. Subsequent sorting permitted transcriptional analysis of both groups. Results and qPCR validation show a strong regulation in damaged neurons versus contralateral controls as well as a moderate regulation in adjacent neurons. Data for damaged neurons reveal an mRNA expression pattern consistent with established upregulated genes like galanin, which supports our approach. Moreover, novel genes were found strongly regulated such as corticotropin-releasing hormone (CRH), providing novel targets for further research. Differential fluorescent neuronal labelling and sorting allows for a clear distinction between primarily damaged neuropathic neurons and “bystanders,” thereby facilitating a more detailed understanding of their respective roles in neuropathic processes in the DRG. PMID:25880204

  6. 38. VIEW OF COTTRELL MAGNETIC IMPULSE GENERATOR ADJACENT TO SIX ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    38. VIEW OF COTTRELL MAGNETIC IMPULSE GENERATOR ADJACENT TO SIX GAP ROTARY RECTIFIER. THIS UNIT GENERATED A MAGNETIC PULSE WHICH WAS TRANSMITTED TO THE COLLECTION PLATES IN THE ELECTROSTATIC PRECIPITATOR CHAMBER. THESE PERIODIC PULSES VIBRATE THE PLATES AND CAUSE PRECIPITATED ARTICLES OF SMOKE AND FLY ASH TO FALL TO THE BOTTOM OF THE PRECIPITATOR CHAMBER. - New York, New Haven & Hartford Railroad, Cos Cob Power Plant, Sound Shore Drive, Greenwich, Fairfield County, CT

  7. Conference room 211, adjacent to commander's quarters, with vault door ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Conference room 211, adjacent to commander's quarters, with vault door at right. Projection area at center is equipped with automatic security drapes. Projection room uses a 45 degree mirror to reflect the image onto the frosted glass screen. Door on far left leads to display area senior battle staff viewing bridge, and the commander's quarters - March Air Force Base, Strategic Air Command, Combat Operations Center, 5220 Riverside Drive, Moreno Valley, Riverside County, CA

  8. Epithelial dysplasia immediately adjacent to oral squamous cell carcinomas.

    PubMed

    Wright, A; Shear, M

    1985-08-01

    A number of workers have attempted to identify dysplastic features which may be predictors of malignant change, by prospective studies of dysplastic lesions. In the present study we have looked at dysplastic changes immediately adjacent to established squamous carcinomas in an attempt to determine whether any predictors can be identified in this way. Eighty cases were included in the study for whom information on tobacco usage was known. Clinical details were recorded. Histological features in epithelium immediately adjacent to the carcinoma were studied in representative sections. Eighteen specific histological characteristics were noted as present or absent. Data were transferred by Conversational Monitoring System (CMS) terminal, processed and analyzed by the Statistical Analysis System (SAS) Computer package. Only 8 patients were non-smokers (10%). Dysplastic changes in adjacent epithelium were frequently multicentric. Changes appear to occur first in the basal layer in the form of disturbance of polarity or basal cell hyperplasia, while other dysplastic features are absent. The feature referred to as basal cell hyperplasia appears, in fact, to represent disturbed epithelial maturation. In 80% of cases increased nucleo-cytoplasmic ratio appears to result from a decrease in cytoplasmic volume rather than increased nuclear size. A defect in RNA synthesis may be a factor. A sharp decrease in inflammatory cells in the lamina propria of adjacent epithelium, compared with that of the carcinoma, was observed. Russell bodies were noted in 5 of the 8 lesions in non-smokers (63%) and in 16 of 72 lesions in smokers (22%) (p less than 0.001; Chi2 17.65). PMID:3928850

  9. 20. Interior view of fuel storage pit or vault adjacent ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. Interior view of fuel storage pit or vault adjacent to Test Cell 9 in Component Test Laboratory (T-27), looking west. Photograph shows upgraded instrumentation, piping, tanks, and technological modifications installed in 1997-99 to accommodate component testing requirements for the Atlas V missile. - Air Force Plant PJKS, Systems Integration Laboratory, Components Test Laboratory, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO

  10. Analysis of Postoperative Thoracolumbar Spine Infections in a Prospective Randomized Controlled Trial Using the Centers for Disease Control Surgical Site Infection Criteria

    PubMed Central

    Takemoto, Richelle C.; Lonner, Baron S.; Andres, Tate M.; Park, Justin J.; Ricart-Hoffiz, Pedro A.; Bendo, John A.; Goldstein, Jeffrey A.; Spivak, Jeffrey M.; Errico, Thomas J.

    2016-01-01

    Introduction Wound infections following spinal surgery place a high toll on both the patient and the healthcare system. Although several large series studies have examined the incidence and distribution of spinal wound infection, the applicability of these studies varies greatly since nearly every study is either retrospective and/or lacks standard inclusion criteria for defining surgical site infection. To address this void, we present results from prospectively gathered thoracolumbar spine surgery data for which the Centers for Disease Control (CDC) criteria were stringently applied to define a surgical site infection (SSI). Methods A prospective randomized trial of 314 patients who underwent multilevel thoracolumbar spinal surgery with instrumentation followed by postoperative drain placement was completed (Takemoto et al., 2015). The trial consisted of two antibiotic arms: one for 24-hours, and the other for the duration of the drain; no differences were found between the arms. All infections meeting CDC criteria for SSI were included. Results A total of 40 infections met CDC criteria for SSI, for an overall incidence of 12.7%. Of these, 20 (50%) were culture-positive. The most common organism was Staphylococcus aureus (4 total: methicillin-sensitive=2; methicillin-resistant=2), followed by coagulase-negative Staphylococcus (3 cases), Propionibacterium acnes and Escherichia coli (2 cases each). Six infections grew multiple organisms, most commonly involving coagulase-negative staphylococcus and enterococcus. Conclusions Our findings indicate that thoracolumbar SSI occurs at the higher end of the range cited in the literature (2-13%), which is largely based on retrospective data not subjected to the inclusivity of SSI as defined by the CDC. The three most common organisms in our analysis (S. aureus, P. acnes, E. coli) are consistent with previous reports. Staphylococcus aureus continues to be the most common causative organism and continued vigilance and

  11. Downscaling site rainfall from daily to 11.25-minute resolution: event, diurnal, seasonal and decadal controls on downscaling parameters

    NASA Astrophysics Data System (ADS)

    McIntyre, Neil; Shi, Shirley; Onof, Christian

    2016-04-01

    Downscaling site rainfall from daily to sub-daily resolution is often approached using the multiplicative discrete random cascade (MDRC) class of models, with mixed success. Questions in any application - for MDRCs or indeed other classes of downscaling model - is to what extent and in what way are model parameters functions of rainfall event type and/or large scale climate controls for example those linked to the El Nino Southern Oscillation (ENSO). These questions underlie the applicability of downscaling models for analysing rainfall and hydrological extremes, in particular for synthesising long-term historical or future sub-daily extremes conditional on historic or projected daily data. Coastal Queensland, Australia, is subject to combinations of multiple weather systems, including tropical cyclones, blocking systems, convective storms, frontal systems and ENSO influences. Using 100 years of fine resolution data from two gauges in central Brisbane, microcanonical MDRC models are fitted to data from 1 day to 11.25 minutes in seven cascade levels, each level dividing the time interval and its rainfall volume into two sub-intervals. Each cascade level involves estimating: the probabilities that all the rainfall observed in a time interval is concentrated in only the first of the two sub-intervals and that all the rainfall observed in a time interval is concentrated in only the second of the two sub-intervals; and also two beta distribution parameters that define the probability of a given division of the rainfall into both sub-intervals. These parameters are found to vary systematically with time of day, rainfall volume, event temporal structure, month of year, and ENSO anomaly. Reasonable downscaling performance is achieved (in terms of replicating extreme values of 11.25 minute rainfall given the observed daily data) by including the parameter dependence on the rainfall volume and event structure, although particular applications may justify development of more

  12. Osmotically regulated floating asymmetric membrane capsule for controlled site-specific delivery of ranitidine hydrochloride: optimization by central composite design.

    PubMed

    Chauhan, Manvendra S; Kumar, Anil; Pathak, Kamla

    2012-12-01

    A nondisintegrating, floating asymmetric membrane capsule (FAMC) was developed to achieve site-specific osmotic flow of a highly water-soluble drug, ranitidine hydrochloride (RHCl), in a controlled manner. Solubility suppression of RHCl was achieved by the common ion effect, using optimized coated sodium chloride as a formulation component. The capsular wall of FAMC was prepared by the phase inversion process wherein the polymeric membrane was precipitated on glass pins by dipping them in a solution of cellulose acetate followed by quenching. Central composite design was utilized to investigate the influence of independent variables, namely, level(s) of membrane former, pore former, and osmogen, on percent cumulative drug release (response). The release mechanism of RHCl through FAMC was confirmed as osmotic pumping. The asymmetry of the membrane was characterized by scanning electron microscopy that revealed a dense nonporous outer region of membrane supported by an inner porous region. Differential scanning calorimetry indicated no incompatibility between the drug and excipients. In vitro drug release in three biorelevant media, pH 2.5 (low fed), pH 4.5 (intermediate fed), and pH 6.5 (high fed), demonstrated pH-independent release of RHCl (P > 0.05). Floating ability for 12 h of the optimized FAMC9 was visually examined during the in vitro release studies that showed maximal drug release with zero-order kinetics (r (2) = 0.9991). Thus, a novel osmotically regulated floating capsular system was developed for site-specific delivery of RHCl. PMID:23104305

  13. The Early Stage Adjacent Disc Degeneration after Percutaneous Vertebroplasty and Kyphoplasty in The Treatment of Osteoporotic VCFs

    PubMed Central

    Qian, Jun; Yang, Huilin; Jing, Juehua; Zhao, Hong; Ni, Li; Tian, Dasheng; Wang, Zhengfei

    2012-01-01

    Background The purpose of this paper is to determine the early incidence of disc de- generation adjacent to the vertebral body of osteoporotic fracture treated with percutaneous vertebroplasty or balloon kyphoplasty and whether adjacent disc degeneration is accelerated by this two procedures. Methods 182 patients with painful vertebral compression fractures were treated. A total of 97 patients were enrolled in this prospective study. 97 patients with a mean age of 65.3 years were classified into control group and surgical treatment group of non-random. 35 patients were in contol group and 62 patients who were performed percutaneous vertebroplasty or balloon kyphoplasty in treatment group. X-ray and Magnetic resonance imaging were done at the first and final visit. The grade of disc degeneration above the fractured vertebral was confirmed by evaluation of bony oedema in the fat suppressed sequences and T2-weighted image of magnetic resonance imaging. The height of degenerative disc was measured on X-ray film. Results All patients were followed up two years after the first visit and the follow-up rate was 90.7% (88/97). The incidence of degeneration of adjacent disc above the fractured vertebral was 29.0% (9/31) in control group and 52.6% (30/57) in treatment group. It presented a statistically significant difference between two groups about the incidence of adjacent disc degeneration (P = 0.033). The percentage of adjacent disc height reduction in control group was 13.5% and 17.6% in treatment group. Statistically significant difference of VAS score and ODI was not found between the first evaluation postoperatively and the final follow-up in treatment group (P>0.05). Conclusions Disc degeneration adjacent to the fractured vertebral is accelerated by VP and BK procedures in the early stage, but clinical outcomes has not been weakened even in the presence of accelerated disc degeneration. PMID:23056283

  14. FMRI evidence of acupoints specificity in two adjacent acupoints.

    PubMed

    Liu, Hua; Xu, Jian-Yang; Li, Lin; Shan, Bao-Ci; Nie, Bin-Bin; Xue, Jing-Quan

    2013-01-01

    Objectives. Acupoint specificity is the foundation of acupuncture treatment. The aim of this study is to investigate whether the acupoint specificity exists in two adjacent acupoints. Design and Setting. Two adjacent real acupoints, LR3 (Taichong) and ST44 (Neiting), and a nearby nonacupoint were selected. Thirty-three health volunteers were divided into three groups in random order, and each group only received acupuncture at one of the three points. While they received acupuncture, fMRI scan was performed. Results. The common cerebral activated areas responding to LR3 and ST44 included the contralateral primary somatosensory area (SI) and ipsilateral cerebellum. Acupuncture at LR3 specifically activated contralateral middle occipital gyrus, ipsilateral medial frontal gyrus, superior parietal lobe, middle temporal gyrus, rostral anterior cingulate cortex (rACC), lentiform nucleus, insula, and contralateral thalamus. Stimulation at ST44 selectively activated ipsilateral secondary somatosensory area (SII), contralateral middle frontal gyrus, inferior frontal gyrus, lingual gyrus, lentiform nucleus, and bilateral posterior cingulate cortex (PCC). Conclusions. Acupuncture at adjacent acupoints elicits distinct cerebral activation patterns, and those specific patterns might be involved in the mechanism of the specific therapeutic effects of different acupoints. PMID:23762172

  15. Divergent viral presentation among human tumors and adjacent normal tissues.

    PubMed

    Cao, Song; Wendl, Michael C; Wyczalkowski, Matthew A; Wylie, Kristine; Ye, Kai; Jayasinghe, Reyka; Xie, Mingchao; Wu, Song; Niu, Beifang; Grubb, Robert; Johnson, Kimberly J; Gay, Hiram; Chen, Ken; Rader, Janet S; Dipersio, John F; Chen, Feng; Ding, Li

    2016-01-01

    We applied a newly developed bioinformatics system called VirusScan to investigate the viral basis of 6,813 human tumors and 559 adjacent normal samples across 23 cancer types and identified 505 virus positive samples with distinctive, organ system- and cancer type-specific distributions. We found that herpes viruses (e.g., subtypes HHV4, HHV5, and HHV6) that are highly prevalent across cancers of the digestive tract showed significantly higher abundances in tumor versus adjacent normal samples, supporting their association with these cancers. We also found three HPV16-positive samples in brain lower grade glioma (LGG). Further, recurrent HBV integration at the KMT2B locus is present in three liver tumors, but absent in their matched adjacent normal samples, indicating that viral integration induced host driver genetic alterations are required on top of viral oncogene expression for initiation and progression of liver hepatocellular carcinoma. Notably, viral integrations were found in many genes, including novel recurrent HPV integrations at PTPN13 in cervical cancer. Finally, we observed a set of HHV4 and HBV variants strongly associated with ethnic groups, likely due to viral sequence evolution under environmental influences. These findings provide important new insights into viral roles of tumor initiation and progression and potential new therapeutic targets. PMID:27339696

  16. Osteochondroma of the hip with adjacent bursal chondromatosis.

    PubMed

    Gould, Elaine S; Baker, Kevin S; Huang, Mingqian; Khan, Fazel; Hoda, Syed

    2014-12-01

    It is well established that irregular bursae can form adjacent to an osteochondroma (bursa exostotica) as a result of mechanical irritation and that these bursae can be complicated by inflammation, hemorrhage, or infection. Bursal chondromatosis is a rare complication, with only seven published cases in the literature according to our searches. We present the case of a 53-year-old female who presented with slowly progressive left hip/thigh pain and was found to have an osteochondroma arising from the lesser trochanter with numerous ossified bodies in the adjacent soft tissues. MRI demonstrated osteochondral bodies in a fluid-filled bursa adjacent to the osteochondroma, with several of the bodies noted to be fairly displaced from the osteochondroma cartilaginous cap. At surgery, the osteochondroma was removed and numerous bodies of varying sizes were excised, some of which were noted to be adherent to the bursal lining and others that were separated/distant from the cartilage cap. The question arises as to whether this process represents bursal chondromatosis resulting from benign neoplasia of cells lining the abnormal bursa, "cartilage shedding" from the osteochondromatous cap, or both. The purpose in presenting this case is to introduce a rare complication of an osteochondroma, demonstrate that soft tissue calcification and osteochondral densities displaced from an underlying osteochondroma are not always the result of sarcomatous degeneration, and provide support for the theory that cells lining a bursa in a nonphysiologic location can undergo benign neoplasia with subsequent formation of osteochondral bodies. PMID:25001874

  17. Divergent viral presentation among human tumors and adjacent normal tissues

    PubMed Central

    Cao, Song; Wendl, Michael C.; Wyczalkowski, Matthew A.; Wylie, Kristine; Ye, Kai; Jayasinghe, Reyka; Xie, Mingchao; Wu, Song; Niu, Beifang; Grubb, Robert; Johnson, Kimberly J.; Gay, Hiram; Chen, Ken; Rader, Janet S.; Dipersio, John F.; Chen, Feng; Ding, Li

    2016-01-01

    We applied a newly developed bioinformatics system called VirusScan to investigate the viral basis of 6,813 human tumors and 559 adjacent normal samples across 23 cancer types and identified 505 virus positive samples with distinctive, organ system- and cancer type-specific distributions. We found that herpes viruses (e.g., subtypes HHV4, HHV5, and HHV6) that are highly prevalent across cancers of the digestive tract showed significantly higher abundances in tumor versus adjacent normal samples, supporting their association with these cancers. We also found three HPV16-positive samples in brain lower grade glioma (LGG). Further, recurrent HBV integration at the KMT2B locus is present in three liver tumors, but absent in their matched adjacent normal samples, indicating that viral integration induced host driver genetic alterations are required on top of viral oncogene expression for initiation and progression of liver hepatocellular carcinoma. Notably, viral integrations were found in many genes, including novel recurrent HPV integrations at PTPN13 in cervical cancer. Finally, we observed a set of HHV4 and HBV variants strongly associated with ethnic groups, likely due to viral sequence evolution under environmental influences. These findings provide important new insights into viral roles of tumor initiation and progression and potential new therapeutic targets. PMID:27339696

  18. Using BRDFs for accurate albedo calculations and adjacency effect corrections

    SciTech Connect

    Borel, C.C.; Gerstl, S.A.W.

    1996-09-01

    In this paper the authors discuss two uses of BRDFs in remote sensing: (1) in determining the clear sky top of the atmosphere (TOA) albedo, (2) in quantifying the effect of the BRDF on the adjacency point-spread function and on atmospheric corrections. The TOA spectral albedo is an important parameter retrieved by the Multi-angle Imaging Spectro-Radiometer (MISR). Its accuracy depends mainly on how well one can model the surface BRDF for many different situations. The authors present results from an algorithm which matches several semi-empirical functions to the nine MISR measured BRFs that are then numerically integrated to yield the clear sky TOA spectral albedo in four spectral channels. They show that absolute accuracies in the albedo of better than 1% are possible for the visible and better than 2% in the near infrared channels. Using a simplified extensive radiosity model, the authors show that the shape of the adjacency point-spread function (PSF) depends on the underlying surface BRDFs. The adjacency point-spread function at a given offset (x,y) from the center pixel is given by the integral of transmission-weighted products of BRDF and scattering phase function along the line of sight.

  19. Regulation of mRNA Abundance by Polypyrimidine Tract-Binding Protein-Controlled Alternate 5′ Splice Site Choice

    PubMed Central

    Hamid, Fursham M.; Makeyev, Eugene V.

    2014-01-01

    Alternative splicing (AS) provides a potent mechanism for increasing protein diversity and modulating gene expression levels. How alternate splice sites are selected by the splicing machinery and how AS is integrated into gene regulation networks remain important questions of eukaryotic biology. Here we report that polypyrimidine tract-binding protein 1 (Ptbp1/PTB/hnRNP-I) controls alternate 5′ and 3′ splice site (5′ss and 3′ss) usage in a large set of mammalian transcripts. A top scoring event identified by our analysis was the choice between competing upstream and downstream 5′ss (u5′ss and d5′ss) in the exon 18 of the Hps1 gene. Hps1 is essential for proper biogenesis of lysosome-related organelles and loss of its function leads to a disease called type 1 Hermansky-Pudlak Syndrome (HPS). We show that Ptbp1 promotes preferential utilization of the u5′ss giving rise to stable mRNAs encoding a full-length Hps1 protein, whereas bias towards d5′ss triggered by Ptbp1 down-regulation generates transcripts susceptible to nonsense-mediated decay (NMD). We further demonstrate that Ptbp1 binds to pyrimidine-rich sequences between the u5′ss and d5′ss and activates the former site rather than repressing the latter. Consistent with this mechanism, u5′ss is intrinsically weaker than d5′ss, with a similar tendency observed for other genes with Ptbp1-induced u5′ss bias. Interestingly, the brain-enriched Ptbp1 paralog Ptbp2/nPTB/brPTB stimulated the u5′ss utilization but with a considerably lower efficiency than Ptbp1. This may account for the tight correlation between Hps1 with Ptbp1 expression levels observed across mammalian tissues. More generally, these data expand our understanding of AS regulation and uncover a post-transcriptional strategy ensuring co-expression of a subordinate gene with its master regulator through an AS-NMD tracking mechanism. PMID:25375251

  20. 49 CFR 214.336 - On-track safety procedures for certain roadway work groups and adjacent tracks.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... through § 214.319 (Working limits, generally) or § 214.329 (Train approach warning provided by watchmen... one of these adjacent controlled tracks has one or more train or other on-track equipment movements... concurrent train or other on-track equipment movements authorized or permitted at a speed over 25 mph,...