Science.gov

Sample records for administration docket id

  1. 19 CFR 351.103 - Central Records Unit and Administrative Protective Order and Dockets Unit.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Order and Dockets Unit. 351.103 Section 351.103 Customs Duties INTERNATIONAL TRADE ADMINISTRATION... Unit and Administrative Protective Order and Dockets Unit. (a) Import Administration's Central Records... Administration's Administrative Protective Order and Dockets Unit (APO/Dockets Unit) is located in Room 1870,...

  2. 19 CFR 351.103 - Central Records Unit and Administrative Protective Order and Dockets Unit.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Order and Dockets Unit. 351.103 Section 351.103 Customs Duties INTERNATIONAL TRADE ADMINISTRATION... Unit and Administrative Protective Order and Dockets Unit. (a) Import Administration's Central Records... Administration's Administrative Protective Order and Dockets Unit (APO/Dockets Unit) is located in Room 1870,...

  3. 19 CFR 351.103 - Central Records Unit and Administrative Protective Order and Dockets Unit.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Order and Dockets Unit. 351.103 Section 351.103 Customs Duties INTERNATIONAL TRADE ADMINISTRATION... Unit and Administrative Protective Order and Dockets Unit. (a) Import Administration's Central Records... (see § 351.104). (b) Import Administration's Administrative Protective Order and Dockets Unit...

  4. 19 CFR 351.103 - Central Records Unit and Administrative Protective Order and Dockets Unit.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Order and Dockets Unit. 351.103 Section 351.103 Customs Duties INTERNATIONAL TRADE ADMINISTRATION... Unit and Administrative Protective Order and Dockets Unit. (a) Import Administration's Central Records... (see § 351.104). (b) Import Administration's Administrative Protective Order and Dockets Unit...

  5. 19 CFR 351.103 - Central Records Unit and Administrative Protective Order and Dockets Unit.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Order and Dockets Unit. 351.103 Section 351.103 Customs Duties INTERNATIONAL TRADE ADMINISTRATION... Unit and Administrative Protective Order and Dockets Unit. (a) Enforcement and Compliance's Central... (see § 351.104). (b) Enforcement and Compliance's Administrative Protective Order and Dockets Unit...

  6. 78 FR 21085 - Establishment of a Public Docket for Administrative Detention Under the Food and Drug...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-09

    ... Administrative Detention Under the Food and Drug Administration Safety and Innovation Act AGENCY: Food and Drug... Administration Safety and Innovation Act (FDASIA). This document is intended to solicit input from all relevant..., and Cosmetic Act (the FD&C Act) (21 U.S.C. 334(g)) to provide FDA administrative detention...

  7. 10 CFR 590.106 - Dockets.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Dockets. 590.106 Section 590.106 Energy DEPARTMENT OF ENERGY (CONTINUED) NATURAL GAS (ECONOMIC REGULATORY ADMINISTRATION) ADMINISTRATIVE PROCEDURES WITH RESPECT TO THE IMPORT AND EXPORT OF NATURAL GAS General Provisions § 590.106 Dockets. The FE...

  8. 10 CFR 590.106 - Dockets.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Dockets. 590.106 Section 590.106 Energy DEPARTMENT OF ENERGY (CONTINUED) NATURAL GAS (ECONOMIC REGULATORY ADMINISTRATION) ADMINISTRATIVE PROCEDURES WITH RESPECT TO THE IMPORT AND EXPORT OF NATURAL GAS General Provisions § 590.106 Dockets. The FE...

  9. 10 CFR 590.106 - Dockets.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Dockets. 590.106 Section 590.106 Energy DEPARTMENT OF ENERGY (CONTINUED) NATURAL GAS (ECONOMIC REGULATORY ADMINISTRATION) ADMINISTRATIVE PROCEDURES WITH RESPECT TO THE IMPORT AND EXPORT OF NATURAL GAS General Provisions § 590.106 Dockets. The FE...

  10. 10 CFR 590.106 - Dockets.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Dockets. 590.106 Section 590.106 Energy DEPARTMENT OF ENERGY (CONTINUED) NATURAL GAS (ECONOMIC REGULATORY ADMINISTRATION) ADMINISTRATIVE PROCEDURES WITH RESPECT TO THE IMPORT AND EXPORT OF NATURAL GAS General Provisions § 590.106 Dockets. The FE...

  11. 10 CFR 590.106 - Dockets.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Dockets. 590.106 Section 590.106 Energy DEPARTMENT OF ENERGY (CONTINUED) NATURAL GAS (ECONOMIC REGULATORY ADMINISTRATION) ADMINISTRATIVE PROCEDURES WITH RESPECT TO THE IMPORT AND EXPORT OF NATURAL GAS General Provisions § 590.106 Dockets. The FE...

  12. Phase I Randomized Clinical Trial of VRC DNA and rAd5 HIV-1 Vaccine Delivery by Intramuscular (IM), Subcutaneous (SC) and Intradermal (ID) Administration (VRC 011)

    PubMed Central

    Enama, Mary E.; Ledgerwood, Julie E.; Novik, Laura; Nason, Martha C.; Gordon, Ingelise J.; Holman, LaSonji; Bailer, Robert T.; Roederer, Mario; Koup, Richard A.; Mascola, John R.; Nabel, Gary J.; Graham, Barney S.

    2014-01-01

    Background Phase 1 evaluation of the VRC HIV DNA and rAd5 vaccines delivered intramuscularly (IM) supported proceeding to a Phase 2 b efficacy study. Here we report comparison of the IM, subcutaneous (SC) and intradermal (ID) routes of administration. Methods Sixty subjects were randomized to 6 schedules to evaluate the IM, SC or ID route for prime injections. Three schedules included DNA primes (Wks 0,4,8) and 3 schedules included rAd5 prime (Wk0); all included rAd5 IM boost (Wk24). DNA vaccine dosage was 4 mg IM or SC, but 0.4 mg ID, while all rAd5 vaccinations were 1010 PU. All injections were administered by needle and syringe. Results Overall, 27/30 subjects completed 3 DNA primes; 30/30 subjects completed rAd5 primes. Mild local pruritus (itchiness), superficial skin lesions and injection site nodules were associated with ID and SC, but not IM injections. All routes induced T-cell and antibody immune responses after rAd5 boosting. Overall, >95% had Env antibody and >80% had Env T-cell responses. Conclusions The pattern of local reactogenicity following ID and SC injections differed from IM injections but all routes were well-tolerated. There was no evidence of an immunogenicity advantage following SC or ID delivery, supporting IM delivery as the preferred route of administration. Trial Registration Clinicaltrials.gov NCT00321061 PMID:24621858

  13. Implementation of the mutual recognition agreement between the United States and the European Community; pharmaceutical GMP's and medical devices; establishment of a public docket and FDA contact points. Food and Drug Administration, HHS. Establishment of a public docket and FDA contact points.

    PubMed

    1999-03-01

    The Food and Drug Administration (FDA) is announcing the establishment of a public docket for the submission and public availability of information concerning the implementation of the Mutual Recognition Agreement (MRA) between the United States and the European Community (EC) in the areas of pharmaceutical good manufacturing practices (GMP's) and medical devices. FDA is also establishing contact points for information covering particular subjects under the MRA implementation, and the agency is making appropriate information available on the FDA web site. PMID:10557625

  14. 49 CFR 389.5 - Regulatory docket.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION FEDERAL MOTOR CARRIER SAFETY REGULATIONS RULEMAKING PROCEDURES-FEDERAL MOTOR CARRIER SAFETY REGULATIONS General § 389.5 Regulatory docket. (a) Information and...

  15. 49 CFR 389.5 - Regulatory docket.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION FEDERAL MOTOR CARRIER SAFETY REGULATIONS RULEMAKING PROCEDURES-FEDERAL MOTOR CARRIER SAFETY REGULATIONS General § 389.5 Regulatory docket. (a) Information and...

  16. 78 FR 58897 - Pipeline Safety: Administrative Procedures; Updates and Technical Corrections

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-25

    ... Notice of Proposed Rulemaking (NPRM) under Docket ID PHMSA-2012-0102, (77 FR 48112) notifying the public...: Administrative Procedures; Updates and Technical Corrections AGENCY: Pipeline and Hazardous Materials Safety... reflect current law, and to make other technical corrections and updates to certain...

  17. 10 CFR 205.15 - Public docket room.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 3 2010-01-01 2010-01-01 false Public docket room. 205.15 Section 205.15 Energy DEPARTMENT OF ENERGY OIL ADMINISTRATIVE PROCEDURES AND SANCTIONS General Provisions § 205.15 Public docket room. There shall be established at the DOE National Office, 12th and Pennsylvania Avenue,...

  18. 49 CFR 601.43 - Opening the docket.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 7 2011-10-01 2011-10-01 false Opening the docket. 601.43 Section 601.43 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ORGANIZATION, FUNCTIONS, AND PROCEDURES Emergency Procedures for Public Transportation Systems § 601.43 Opening the docket....

  19. 78 FR 40142 - Office of Managing Director Creates Docket for Petitions Seeking Review of Application Fee Refund...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-03

    ...The Office of Managing Director has created the new docket shown above for petitions seeking review of application fee refund denials. Consolidating all these related pleadings in a separate docket will promote administrative efficiency and enhance public accessibility to these...

  20. 75 FR 38114 - [Docket ID FEMA-2010-0008

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-01

    ...) After Action Report (AAR) Improvement Plan (IP). SUMMARY: The Federal Emergency Management Agency (FEMA... SECURITY Federal Emergency Management Agency Agency Information Collection Activities: Submission for OMB...) After Action Report (AAR) Improvement Plan (IP) AGENCY: Federal Emergency Management Agency, DHS....

  1. 77 FR 5293 - Establishment of an Emergency Relief Docket for Calendar Year 2012

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-02

    ..., regulations, or standards during an emergency situation or event. 74 FR 23329. That direct final rule became... Federal Railroad Administration Establishment of an Emergency Relief Docket for Calendar Year 2012 AGENCY...: This Notice announces the establishment of FRA's emergency relief docket (ERD) for calendar year...

  2. 46 CFR 502.271 - Special docket application for permission to refund or waive freight charges.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 9 2011-10-01 2011-10-01 false Special docket application for permission to refund or waive freight charges. 502.271 Section 502.271 Shipping FEDERAL MARITIME COMMISSION GENERAL AND ADMINISTRATIVE PROVISIONS RULES OF PRACTICE AND PROCEDURE Refund or Waiver of Freight Charges § 502.271 Special docket application for permission to...

  3. 75 FR 60119 - Registration Review; Antimicrobial Pesticide Dockets Opened for Review and Comment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-29

    ... for the pesticides listed in the table in Unit III.A. With this document, EPA is opening the public comment period for these registration reviews. Registration review is EPA's periodic review of pesticide... docket identification (ID) number for the specific pesticide of interest provided in the table in...

  4. 78 FR 21120 - Registration Review; Pesticide Dockets Opened for Review and Comment and Other Docket Acts...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-09

    ...; Correction AGENCY: Environmental Protection Agency (EPA). ACTION: Notice; correction. SUMMARY: EPA issued a... (7508P), Office of Pesticide Programs, Environmental Protection Agency, 1200 Pennsylvania Ave. NW... Programs Regulatory Public Docket (OPP Docket) in the Environmental Protection Agency Docket Center...

  5. 49 CFR 601.42 - Emergency relief docket.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 7 2012-10-01 2012-10-01 false Emergency relief docket. 601.42 Section 601.42 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ORGANIZATION, FUNCTIONS, AND PROCEDURES Emergency Procedures for...

  6. 49 CFR 601.44 - Posting to the docket.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 7 2010-10-01 2010-10-01 false Posting to the docket. 601.44 Section 601.44 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ORGANIZATION, FUNCTIONS, AND PROCEDURES Emergency Procedures for...

  7. 49 CFR 601.42 - Emergency relief docket.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 7 2010-10-01 2010-10-01 false Emergency relief docket. 601.42 Section 601.42 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ORGANIZATION, FUNCTIONS, AND PROCEDURES Emergency Procedures for...

  8. 49 CFR 601.42 - Emergency relief docket.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 7 2014-10-01 2014-10-01 false Emergency relief docket. 601.42 Section 601.42 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ORGANIZATION, FUNCTIONS, AND PROCEDURES Emergency Procedures for...

  9. 49 CFR 601.42 - Emergency relief docket.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 7 2013-10-01 2013-10-01 false Emergency relief docket. 601.42 Section 601.42 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ORGANIZATION, FUNCTIONS, AND PROCEDURES Emergency Procedures for...

  10. 49 CFR 601.43 - Opening the docket.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 7 2013-10-01 2013-10-01 false Opening the docket. 601.43 Section 601.43 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ORGANIZATION, FUNCTIONS, AND PROCEDURES Emergency Procedures for...

  11. 49 CFR 601.43 - Opening the docket.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 7 2014-10-01 2014-10-01 false Opening the docket. 601.43 Section 601.43 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ORGANIZATION, FUNCTIONS, AND PROCEDURES Emergency Procedures for...

  12. 49 CFR 601.44 - Posting to the docket.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 7 2014-10-01 2014-10-01 false Posting to the docket. 601.44 Section 601.44 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ORGANIZATION, FUNCTIONS, AND PROCEDURES Emergency Procedures for...

  13. 49 CFR 601.44 - Posting to the docket.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 7 2012-10-01 2012-10-01 false Posting to the docket. 601.44 Section 601.44 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ORGANIZATION, FUNCTIONS, AND PROCEDURES Emergency Procedures for...

  14. 49 CFR 601.44 - Posting to the docket.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 7 2013-10-01 2013-10-01 false Posting to the docket. 601.44 Section 601.44 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ORGANIZATION, FUNCTIONS, AND PROCEDURES Emergency Procedures for...

  15. 49 CFR 601.43 - Opening the docket.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 7 2012-10-01 2012-10-01 false Opening the docket. 601.43 Section 601.43 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ORGANIZATION, FUNCTIONS, AND PROCEDURES Emergency Procedures for...

  16. 49 CFR 601.42 - Emergency relief docket.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 7 2011-10-01 2011-10-01 false Emergency relief docket. 601.42 Section 601.42 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ORGANIZATION, FUNCTIONS, AND PROCEDURES Emergency Procedures for Public Transportation Systems § 601.42 Emergency...

  17. 49 CFR 601.44 - Posting to the docket.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 7 2011-10-01 2011-10-01 false Posting to the docket. 601.44 Section 601.44 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ORGANIZATION, FUNCTIONS, AND PROCEDURES Emergency Procedures for Public Transportation Systems § 601.44 Posting to...

  18. 49 CFR 601.43 - Opening the docket.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 7 2010-10-01 2010-10-01 false Opening the docket. 601.43 Section 601.43 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ORGANIZATION, FUNCTIONS, AND PROCEDURES Emergency Procedures for...

  19. 29 CFR 801.64 - Notice of docketing.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 3 2012-07-01 2012-07-01 false Notice of docketing. 801.64 Section 801.64 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR OTHER LAWS APPLICATION OF THE EMPLOYEE POLYGRAPH PROTECTION ACT OF 1988 Administrative Proceedings Referral for...

  20. 29 CFR 801.64 - Notice of docketing.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 3 2013-07-01 2013-07-01 false Notice of docketing. 801.64 Section 801.64 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR OTHER LAWS APPLICATION OF THE EMPLOYEE POLYGRAPH PROTECTION ACT OF 1988 Administrative Proceedings Referral for...

  1. 29 CFR 801.64 - Notice of docketing.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 3 2014-07-01 2014-07-01 false Notice of docketing. 801.64 Section 801.64 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR OTHER LAWS APPLICATION OF THE EMPLOYEE POLYGRAPH PROTECTION ACT OF 1988 Administrative Proceedings Referral for...

  2. 29 CFR 801.64 - Notice of docketing.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 3 2011-07-01 2011-07-01 false Notice of docketing. 801.64 Section 801.64 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR OTHER LAWS APPLICATION OF THE EMPLOYEE POLYGRAPH PROTECTION ACT OF 1988 Administrative Proceedings Referral for...

  3. 29 CFR 801.64 - Notice of docketing.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Notice of docketing. 801.64 Section 801.64 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR OTHER LAWS APPLICATION OF THE EMPLOYEE POLYGRAPH PROTECTION ACT OF 1988 Administrative Proceedings Referral for...

  4. 29 CFR 500.225 - Notice of docketing.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Notice of docketing. 500.225 Section 500.225 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR REGULATIONS MIGRANT AND SEASONAL AGRICULTURAL WORKER PROTECTION Administrative Proceedings Referral for Hearing § 500.225 Notice...

  5. 29 CFR 500.225 - Notice of docketing.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 3 2011-07-01 2011-07-01 false Notice of docketing. 500.225 Section 500.225 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR REGULATIONS MIGRANT AND SEASONAL AGRICULTURAL WORKER PROTECTION Administrative Proceedings Referral for Hearing § 500.225 Notice...

  6. 75 FR 4449 - Requested Administrative Waiver of the Coastwise Trade Laws; Correction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-27

    ...On January 15, 2010, the Maritime Administration published notice of administrative waiver of the coastwise trade laws. There was an inadvertent error in the docket number. The correct docket number, MARAD-2010-0001, heads this...

  7. 75 FR 4449 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-27

    ...On January 15, 2010, the Maritime Administration published notice of administrative waiver of the coastwise trade laws. There was an inadvertent error in the docket number. The correct docket number, MARAD-2010-0002, heads this...

  8. "Where I Came From, Where I Am Now, and Where I'd Like to Be": Aspiring Administrators Reflect on Issues Related to Equity, Diversity, and Social Justice

    ERIC Educational Resources Information Center

    Hernandez, Frank; Marshall, Joanne M.

    2009-01-01

    This study explores student reflections about issues related to equity, diversity, and social justice from an educational foundations course. Online reflections and course assignments were analyzed from 15 aspiring administrators for patterns. Findings indicate that (1) students were willing to engage and reflect on their experiences and cultural…

  9. 49 CFR 190.305 - Regulatory dockets.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., docketed material may be accessed at http://www.regulations.gov. Public comments also may be submitted at http://www.regulations.gov. Comment submissions must identify the docket number. You may also...

  10. 24 CFR 10.4 - Rules docket.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Rules docket. 10.4 Section 10.4... RULEMAKING: POLICY AND PROCEDURES General § 10.4 Rules docket. (a) All documents relating to rulemaking..., final rules and general notices are maintained in the Rules Docket Room (Room 5218), Department...

  11. 10 CFR 110.102 - Hearing docket.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Hearing docket. 110.102 Section 110.102 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) EXPORT AND IMPORT OF NUCLEAR EQUIPMENT AND MATERIAL Hearings § 110.102 Hearing docket. For each hearing, the Secretary will maintain a docket which will include the...

  12. 49 CFR 845.50 - Public docket.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 7 2011-10-01 2011-10-01 false Public docket. 845.50 Section 845.50... RULES OF PRACTICE IN TRANSPORTATION; ACCIDENT/INCIDENT HEARINGS AND REPORTS Public Record § 845.50 Public docket. (a) The public docket shall include all factual information concerning the...

  13. 49 CFR 845.50 - Public docket.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 7 2013-10-01 2013-10-01 false Public docket. 845.50 Section 845.50... RULES OF PRACTICE IN TRANSPORTATION; ACCIDENT/INCIDENT HEARINGS AND REPORTS Public Record § 845.50 Public docket. (a) The public docket shall include all factual information concerning the...

  14. 7 CFR 900.54 - Docket number.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Docket number. 900.54 Section 900.54 Agriculture... Governing Proceedings on Petitions To Modify or To Be Exempted From Marketing Orders § 900.54 Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by...

  15. 7 CFR 1.414 - Docket number.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 1 2014-01-01 2014-01-01 false Docket number. 1.414 Section 1.414 Agriculture Office... Conservation and Shortage Relief Act of 1990 (16 U.S.C. 620 et seq.) § 1.414 Docket number. Each proceeding, following its institution, shall be assigned a docket number by the Hearing Clerk, and thereafter...

  16. 7 CFR 900.54 - Docket number.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Docket number. 900.54 Section 900.54 Agriculture... Governing Proceedings on Petitions To Modify or To Be Exempted From Marketing Orders § 900.54 Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by...

  17. 7 CFR 1.134 - Docket number.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Docket number. 1.134 Section 1.134 Agriculture Office... Adjudicatory Proceedings Instituted by the Secretary Under Various Statutes § 1.134 Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by the Hearing...

  18. 7 CFR 900.54 - Docket number.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Docket number. 900.54 Section 900.54 Agriculture... Governing Proceedings on Petitions To Modify or To Be Exempted From Marketing Orders § 900.54 Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by...

  19. 7 CFR 1.134 - Docket number.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 1 2012-01-01 2012-01-01 false Docket number. 1.134 Section 1.134 Agriculture Office... Adjudicatory Proceedings Instituted by the Secretary Under Various Statutes § 1.134 Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by the Hearing...

  20. 7 CFR 1.414 - Docket number.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 1 2012-01-01 2012-01-01 false Docket number. 1.414 Section 1.414 Agriculture Office... Conservation and Shortage Relief Act of 1990 (16 U.S.C. 620 et seq.) § 1.414 Docket number. Each proceeding, following its institution, shall be assigned a docket number by the Hearing Clerk, and thereafter...

  1. 7 CFR 1.414 - Docket number.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 1 2011-01-01 2011-01-01 false Docket number. 1.414 Section 1.414 Agriculture Office... Conservation and Shortage Relief Act of 1990 (16 U.S.C. 620 et seq.) § 1.414 Docket number. Each proceeding, following its institution, shall be assigned a docket number by the Hearing Clerk, and thereafter...

  2. 7 CFR 900.54 - Docket number.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Docket number. 900.54 Section 900.54 Agriculture... Governing Proceedings on Petitions To Modify or To Be Exempted From Marketing Orders § 900.54 Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by...

  3. 7 CFR 1200.6 - Docket number.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 10 2013-01-01 2013-01-01 false Docket number. 1200.6 Section 1200.6 Agriculture... Governing Proceedings To Formulate and Amend an Order § 1200.6 Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by the hearing clerk and thereafter...

  4. 7 CFR 900.5 - Docket number.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Docket number. 900.5 Section 900.5 Agriculture... Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by the hearing clerk and thereafter the proceeding may be referred to by such number....

  5. 7 CFR 900.5 - Docket number.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Docket number. 900.5 Section 900.5 Agriculture... Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by the hearing clerk and thereafter the proceeding may be referred to by such number....

  6. 7 CFR 1200.6 - Docket number.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Docket number. 1200.6 Section 1200.6 Agriculture... Governing Proceedings To Formulate and Amend an Order § 1200.6 Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by the hearing clerk and thereafter...

  7. 7 CFR 900.5 - Docket number.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Docket number. 900.5 Section 900.5 Agriculture... Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by the hearing clerk and thereafter the proceeding may be referred to by such number....

  8. 7 CFR 1.414 - Docket number.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false Docket number. 1.414 Section 1.414 Agriculture Office... Conservation and Shortage Relief Act of 1990 (16 U.S.C. 620 et seq.) § 1.414 Docket number. Each proceeding, following its institution, shall be assigned a docket number by the Hearing Clerk, and thereafter...

  9. 7 CFR 1.134 - Docket number.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false Docket number. 1.134 Section 1.134 Agriculture Office... Adjudicatory Proceedings Instituted by the Secretary Under Various Statutes § 1.134 Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by the Hearing...

  10. 7 CFR 1200.6 - Docket number.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 10 2014-01-01 2014-01-01 false Docket number. 1200.6 Section 1200.6 Agriculture... Governing Proceedings To Formulate and Amend an Order § 1200.6 Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by the hearing clerk and thereafter...

  11. 7 CFR 1200.6 - Docket number.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 10 2011-01-01 2011-01-01 false Docket number. 1200.6 Section 1200.6 Agriculture... Governing Proceedings To Formulate and Amend an Order § 1200.6 Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by the hearing clerk and thereafter...

  12. 7 CFR 900.54 - Docket number.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Docket number. 900.54 Section 900.54 Agriculture... Governing Proceedings on Petitions To Modify or To Be Exempted From Marketing Orders § 900.54 Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by...

  13. 7 CFR 1.414 - Docket number.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Docket number. 1.414 Section 1.414 Agriculture Office... Conservation and Shortage Relief Act of 1990 (16 U.S.C. 620 et seq.) § 1.414 Docket number. Each proceeding, following its institution, shall be assigned a docket number by the Hearing Clerk, and thereafter...

  14. 7 CFR 1.134 - Docket number.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 1 2014-01-01 2014-01-01 false Docket number. 1.134 Section 1.134 Agriculture Office... Adjudicatory Proceedings Instituted by the Secretary Under Various Statutes § 1.134 Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by the Hearing...

  15. 7 CFR 900.5 - Docket number.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Docket number. 900.5 Section 900.5 Agriculture... Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by the hearing clerk and thereafter the proceeding may be referred to by such number....

  16. 7 CFR 900.5 - Docket number.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Docket number. 900.5 Section 900.5 Agriculture... Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by the hearing clerk and thereafter the proceeding may be referred to by such number....

  17. 7 CFR 1200.6 - Docket number.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 10 2012-01-01 2012-01-01 false Docket number. 1200.6 Section 1200.6 Agriculture... Governing Proceedings To Formulate and Amend an Order § 1200.6 Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by the hearing clerk and thereafter...

  18. 7 CFR 1.134 - Docket number.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 1 2011-01-01 2011-01-01 false Docket number. 1.134 Section 1.134 Agriculture Office... Adjudicatory Proceedings Instituted by the Secretary Under Various Statutes § 1.134 Docket number. Each proceeding, immediately following its institution, shall be assigned a docket number by the Hearing...

  19. 76 FR 31388 - Idaho Disaster #ID-00014

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-31

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Idaho Disaster ID-00014 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for Public Assistance Only for...

  20. 75 FR 45682 - Idaho Disaster #ID-00010

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-03

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Idaho Disaster ID-00010 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for Public Assistance Only for...

  1. 77 FR 5088 - Notice of Establishment of Emergency Relief Docket for Calendar Year 2012

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-01

    ... 604.2(f) of FTA's charter rule (73 FR 2325, Jan. 14, 2008), grantees and subgrantees may assist with... Federal Transit Administration Notice of Establishment of Emergency Relief Docket for Calendar Year 2012 AGENCY: Federal Transit Administration (FTA), DOT. ACTION: Notice. SUMMARY: The Federal...

  2. 78 FR 11268 - Notice of Establishment of Emergency Relief Docket for Calendar Year 2013

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-15

    ... charter rule (73 FR 2325, Jan. 14, 2008), grantees and subgrantees may assist with evacuations or other... Federal Transit Administration Notice of Establishment of Emergency Relief Docket for Calendar Year 2013 AGENCY: Federal Transit Administration (FTA), DOT. ACTION: Notice SUMMARY: The Federal...

  3. 22 CFR 102.27 - Docket.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... DEPARTMENT OF STATE ECONOMIC AND OTHER FUNCTIONS CIVIL AVIATION Recommendations to the President Under Section 801 of the Federal Aviation Act of 1958 § 102.27 Docket. (a) All comments submitted under this subpart shall reference the number of the Civil Aeronautics Board docket relating to the proceeding...

  4. 22 CFR 102.27 - Docket.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... DEPARTMENT OF STATE ECONOMIC AND OTHER FUNCTIONS CIVIL AVIATION Recommendations to the President Under Section 801 of the Federal Aviation Act of 1958 § 102.27 Docket. (a) All comments submitted under this subpart shall reference the number of the Civil Aeronautics Board docket relating to the proceeding...

  5. 22 CFR 102.27 - Docket.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... DEPARTMENT OF STATE ECONOMIC AND OTHER FUNCTIONS CIVIL AVIATION Recommendations to the President Under Section 801 of the Federal Aviation Act of 1958 § 102.27 Docket. (a) All comments submitted under this subpart shall reference the number of the Civil Aeronautics Board docket relating to the proceeding...

  6. 49 CFR 211.5 - Regulatory docket.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Jersey Avenue, SE., Washington, DC 20590. Copies of docketed materials may be obtained upon payment of... electronic storage or printing. There is no charge for this service. (b) Records of the Federal Railroad... FRA's Docket Office, 1200 New Jersey Avenue, SE., Washington, DC 20590. Any person may...

  7. 49 CFR 211.5 - Regulatory docket.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Jersey Avenue, SE., Washington, DC 20590. Copies of docketed materials may be obtained upon payment of... electronic storage or printing. There is no charge for this service. (b) Records of the Federal Railroad... FRA's Docket Office, 1200 New Jersey Avenue, SE., Washington, DC 20590. Any person may...

  8. Not Another ID Model.

    ERIC Educational Resources Information Center

    Roberts, Stephanie; Conn, Cynthia; Lohr, Linda; Hunt, Erin; Duffy, Annmari

    2003-01-01

    Presents a meta-model of instructional design (ID) that integrates content covered in instructional technology graduate programs and explores the impact of an instructional designer's perspective on designs. Discusses the theoretical background; instructional strategies; educational philosophies; paradigm shifts; behaviorist, constructivist, and…

  9. 20 CFR 802.302 - Docketing of appeals.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Docketing of appeals. 802.302 Section 802.302 Employees' Benefits BENEFITS REVIEW BOARD, DEPARTMENT OF LABOR RULES OF PRACTICE AND PROCEDURE Procedure for Review Action by the Board § 802.302 Docketing of appeals. (a) Maintenance of dockets. A docket of...

  10. 33 CFR 1.05-25 - Public docket.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Public docket. 1.05-25 Section 1... PROVISIONS Rulemaking § 1.05-25 Public docket. (a) A public docket is maintained electronically for each... Register. Each docket contains copies of every rulemaking document published for the project,...

  11. 33 CFR 1.05-25 - Public docket.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Public docket. 1.05-25 Section 1... PROVISIONS Rulemaking § 1.05-25 Public docket. (a) A public docket is maintained electronically for each... Register. Each docket contains copies of every rulemaking document published for the project,...

  12. 76 FR 38166 - Registration Review; Pesticide Dockets Opened for Review and Comment and Other Docket Actions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-29

    ... review docket for cucumber beetle attractant. This pesticide does not currently have any actively... cucumber beetle attractant because this pesticide is not included in any products actively registered...

  13. Successful I.D. Encounters.

    ERIC Educational Resources Information Center

    Poorman, Margaret J.

    Instructional Development (I.D.) encounters are dependent for success on such variables as power, politics, promotion, and organizational placement. I.D. consultants must be aware of power bases or orientation of other personnel and clients, e.g., these four "power personalities" which affect their efforts in managing I.D. encounters: the gate…

  14. 7 CFR 1944.412 - Docket preparation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...) PROGRAM REGULATIONS (CONTINUED) HOUSING Self-Help Technical Assistance Grants § 1944.412 Docket...-Help Technical Assistance Grant Agreement (Exhibit A) 2 1 1-O 1-C Any Personnel Forms to be used 2...

  15. 7 CFR 1944.412 - Docket preparation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...) PROGRAM REGULATIONS (CONTINUED) HOUSING Self-Help Technical Assistance Grants § 1944.412 Docket...-Help Technical Assistance Grant Agreement (Exhibit A) 2 1 1-O 1-C Any Personnel Forms to be used 2...

  16. 7 CFR 1944.412 - Docket preparation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...) PROGRAM REGULATIONS (CONTINUED) HOUSING Self-Help Technical Assistance Grants § 1944.412 Docket...-Help Technical Assistance Grant Agreement (Exhibit A) 2 1 1-O 1-C Any Personnel Forms to be used 2...

  17. 44 CFR 66.3 - Establishment of community case file and flood elevation study docket.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... case file and flood elevation study docket. 66.3 Section 66.3 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY INSURANCE AND HAZARD MITIGATION... placed in the community file. Even if a map is administratively rescinded or withdrawn after notice...

  18. 44 CFR 66.3 - Establishment of community case file and flood elevation study docket.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... case file and flood elevation study docket. 66.3 Section 66.3 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY INSURANCE AND HAZARD MITIGATION... placed in the community file. Even if a map is administratively rescinded or withdrawn after notice...

  19. 44 CFR 66.3 - Establishment of community case file and flood elevation study docket.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... case file and flood elevation study docket. 66.3 Section 66.3 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY INSURANCE AND HAZARD MITIGATION... placed in the community file. Even if a map is administratively rescinded or withdrawn after notice...

  20. IDS transfer from overexpressing cells to IDS-deficient cells.

    PubMed

    Millat, G; Froissart, R; Maire, I; Bozon, D

    1997-02-01

    Iduronate sulfatase (IDS) is responsible for mucopolysaccharidosis type II, a rare recessive X-linked lysosomal storage disease. The aim of this work was to test the ability of overexpressing cells to transfer IDS to deficient cells. In the first part of our work, IDS processing steps were compared in fibroblasts, COS cells, and lymphoblastoid cell lines and shown to be identical: the two precursor forms (76 and 90 kDa) were processed by a series of intermediate forms to the 55- and 45-kDa mature polypeptides. Then IDS transfer to IDS-deficient cells was tested either by incubation with cell-free medium of overexpressing cells or by coculture. Endocytosis and coculture experiments between transfected L beta and deleted fibroblasts showed that IDS transfer occurred preferentially by cell-to-cell contact as IDS precursors are poorly secreted by transfected L beta. The 76- and 62-kDa IDS polypeptides transferred to deleted fibroblasts were correctly processed to the mature 55- and 45-kDa forms. L beta were not able to internalize the 90-kDa phosphorylated precursor forms excreted in large amounts in the medium of overexpressing fibroblasts. Enzyme transfer occurred only by cell-to-cell contact, but the precursor forms transferred in L beta after cell-to-cell contact were not processed. This absence of maturation was probably due to a mistargeting of IDS precursors in these cells. PMID:9024795

  1. 14 CFR 406.113 - Filing documents with the Docket Management System (DMS) and sending documents to the...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Filing documents with the Docket Management System (DMS) and sending documents to the administrative law judge and Assistant Chief Counsel for Litigation. 406.113 Section 406.113 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT...

  2. 14 CFR 406.113 - Filing documents with the Docket Management System (DMS) and sending documents to the...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Filing documents with the Docket Management System (DMS) and sending documents to the administrative law judge and Assistant Chief Counsel for Litigation. 406.113 Section 406.113 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT...

  3. DHSIsotopeID

    SciTech Connect

    Mitchell, Dean J.

    2007-12-18

    DHSIsotopeID is an application designed to read and analyze radiation measurement files taken by radiation measurement files taken by radiation portal monitors, and in particular, by the advanced spectroscopic portals. It requires that the data files be in the N42 file format, compliant with the interface control documents for DNDO radiation measurement files. It carries out an automated analysis to determine which isotopes are present in the spectra, and then presents the results in graphical form to the user. It also enables further post-processing and analysis, for example by performing further analysis on selected regions of interest of the spectrum, as designated by the user via the graphical interface.

  4. DHSIsotopeID

    2007-12-18

    DHSIsotopeID is an application designed to read and analyze radiation measurement files taken by radiation measurement files taken by radiation portal monitors, and in particular, by the advanced spectroscopic portals. It requires that the data files be in the N42 file format, compliant with the interface control documents for DNDO radiation measurement files. It carries out an automated analysis to determine which isotopes are present in the spectra, and then presents the results in graphicalmore » form to the user. It also enables further post-processing and analysis, for example by performing further analysis on selected regions of interest of the spectrum, as designated by the user via the graphical interface.« less

  5. 78 FR 65555 - Establishment of Class E Airspace; Salmon, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-01

    ... Federal Aviation Administration 14 CFR Part 71 Establishment of Class E Airspace; Salmon, ID AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action establishes Class E... the establishment of Class E en route airspace. Class E airspace designations are published...

  6. 76 FR 70865 - Modification of Class E Airspace; Driggs, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-16

    ...), 40103, 40113, 40120; E. O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 0 2. The... Federal Aviation Administration 14 CFR Part 71 Modification of Class E Airspace; Driggs, ID AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action modifies Class...

  7. ID-69 Sodium drain experiments

    SciTech Connect

    Johnston, D.C.

    1996-09-19

    This paper describes experiments to determine the sodium retention and drainage from the two key areas of an ID-69. This information is then used as the initiation point for guidelines of how to proceed with washing an ID-69 in the IEM Cell Sodium Removal System.

  8. IDS contribution to ITRF2008

    NASA Astrophysics Data System (ADS)

    Valette, J.; Lemoine, F.; Ferrage, P.; Altamimi, Z.; Willis, P.; Stepanek, P.; Otten, M.; Govind, R.; Kuzin, S.; Le Bail, K.; Moore, P.; Yaya, P.; Soudarin, L.

    2009-12-01

    The International DORIS Service (IDS), in operation since 2003, submitted three sets of solutions to ITRF2005 from the IGN/JPL, LEGOS/CLS, and INASAN analysis centers, but no DORIS technique combination. Since that time new analysis centers have become operational including the Geodetic Observatory Pecny (GOP), the European Space Operations Center (ESOC), Geoscience Australia (GAU), the NASA Goddard Space Flight Center (GSC), and the University of Newcastle (NCL). These analysis centers run different software, including Gypsy (IGN & INASAN), GINS (LCA), Bernese (GOP), NAPEOS (ESOC), GEODYN (Geoscience Australia and NASA GSFC) and FAUST (NCL). In order to contribute to ITRF2008, seven analysis centers processed DORIS data from TOPEX/Poseidon, SPOT2, SPOT3, SPOT4, SPOT5, and ENVISAT from 1992 to 2008, producing weekly SINEX solutions or normal equations. The weekly SINEX files from seven AC’s were processed with the CATREF software. Three iterations of an IDS weekly combined time series were completed. The IDS-1, and IDS-2 combinations were preliminary station-only solutions. In the final combination, IDS-3, both stations and the Earth Orientation Paramters (EOP’s) were adjusted. Between each of the IDS combinations, the combination strategy (station filtering, outliers, weighting, scale or geocenter contributions) was improved and the AC’s SINEX series were refined. Some series were extended in data span while others were recomputed to correct anomalies or to improve the quality of the submissions, based on feedback from the combination analyses and intercenter comparisons. For example in IDS-1, both the GAU and GSC solutions were affected by a 20 mm scale offset that was removed in IDS-2 and IDS-3 after the application of improved troposphere modelling in the GEODYN software. The analysis for IDS-1 showed a higher station position WRMS in the vicinity of the high solar flux periods (late 2001-2002). Consequently for IDS-2, several AC’s (LCA, GAU, GSC

  9. 78 FR 8498 - [Docket ID DoD-2013-OS-0016

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-06

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF DEFENSE Office of the Secretary Manual for Courts-Martial; Proposed Amendments AGENCY: Joint Service Committee on Military Justice (JSC), DoD. ACTION: Annual Review of the Manual for Courts-Martial, United States....

  10. 7 CFR 1944.412 - Docket preparation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 13 2010-01-01 2009-01-01 true Docket preparation. 1944.412 Section 1944.412 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, RURAL BUSINESS-COOPERATIVE SERVICE, RURAL UTILITIES SERVICE, AND FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE (CONTINUED) PROGRAM REGULATIONS (CONTINUED)...

  11. 7 CFR 1944.412 - Docket preparation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 13 2011-01-01 2009-01-01 true Docket preparation. 1944.412 Section 1944.412 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, RURAL BUSINESS-COOPERATIVE SERVICE, RURAL UTILITIES SERVICE, AND FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE (CONTINUED) PROGRAM REGULATIONS (CONTINUED)...

  12. 49 CFR 845.50 - Public docket.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 7 2010-10-01 2010-10-01 false Public docket. 845.50 Section 845.50 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL TRANSPORTATION SAFETY BOARD RULES OF PRACTICE IN TRANSPORTATION; ACCIDENT/INCIDENT HEARINGS AND REPORTS Public Record §...

  13. 40 CFR 155.32 - Public docket.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... under development. (2) If the Agency notifies registrants privately in accordance with 40 CFR 154.21 that one or more risk criteria set forth in 40 CFR 154.7 (leading to a special review) may have been... 40 Protection of Environment 24 2014-07-01 2014-07-01 false Public docket. 155.32 Section...

  14. Social Science Docket, 2000-2001.

    ERIC Educational Resources Information Center

    Singer, Alan, Ed.

    2001-01-01

    A joint publication of the New York and New Jersey State Councils for the Social Studies, "Social Science Docket" presents K-12 teachers with resources covering the social science disciplines, including history, economics, political science, sociology, geography, anthropology, and psychology. Each issue includes theme-related and non-themed…

  15. 14 CFR 406.113 - Filing documents with the Docket Management System (DMS) and sending documents to the...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Filing documents with the Docket Management System (DMS) and sending documents to the administrative law judge and Assistant Chief Counsel for Litigation. 406.113 Section 406.113 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL...

  16. 14 CFR 406.113 - Filing documents with the Docket Management System (DMS) and sending documents to the...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Filing documents with the Docket Management System (DMS) and sending documents to the administrative law judge and Assistant Chief Counsel for Litigation. 406.113 Section 406.113 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL...

  17. 14 CFR 406.113 - Filing documents with the Docket Management System (DMS) and sending documents to the...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Filing documents with the Docket Management System (DMS) and sending documents to the administrative law judge and Assistant Chief Counsel for Litigation. 406.113 Section 406.113 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL...

  18. 76 FR 59672 - Notice of Change In IC Docket Numbering Policy

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-27

    ... the Commission is modifying the numbering system for the docket prefix IC. These IC docket notices... Commission adopted the current IC docket prefix \\1\\ in order to properly track any comments it receives in... the way the IC docket prefix is set up. Beginning on October 1, 2011, IC dockets will continue to...

  19. 76 FR 22863 - Designation for the Lewiston, ID Area

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-25

    ... hours (7 CFR 1.27(c)). SUPPLEMENTARY INFORMATION: In the January 4, 2011, Federal Register (76 FR 317... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Grain Inspection, Packers and Stockyards Administration Designation for the Lewiston, ID Area...

  20. 77 FR 56608 - Designation for the Pocatello, ID; Evansville, IN; and Salt Lake City, UT Areas

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-13

    ... the March 5, 2012 Federal Register (76 FR 2012-5245), GIPSA requested applications for designation to... Grain Inspection, Packers and Stockyards Administration Designation for the Pocatello, ID; Evansville... start end Idaho Pocatello, ID (208) 233-8303.... 10/1/2012 9/30/2015 Ohio Valley Evansville, IN...

  1. Threshold extended ID3 algorithm

    NASA Astrophysics Data System (ADS)

    Kumar, A. B. Rajesh; Ramesh, C. Phani; Madhusudhan, E.; Padmavathamma, M.

    2012-04-01

    Information exchange over insecure networks needs to provide authentication and confidentiality to the database in significant problem in datamining. In this paper we propose a novel authenticated multiparty ID3 Algorithm used to construct multiparty secret sharing decision tree for implementation in medical transactions.

  2. ISS/IDS Detector Study

    SciTech Connect

    Cervera-Villanueva, A.

    2008-02-21

    This article summarises the results obtained by the detector working group of the 'International Scooping Study' (ISS) of a future neutrino oscillations facility. Special emphasis is put on far detectors, for which some of the main issues are identified. A detector R and D strategy in the context of the 'International Design Study' (IDS) for a neutrino factory is also presented.

  3. 76 FR 17646 - Registration Review; Pesticide Dockets Opened for Review and Comment and Other Docket Actions...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-30

    ... has established registration review dockets for the ] pesticides listed in the table in Unit III.A.... Registration review is EPA's periodic review of pesticide registrations to ensure that each pesticide continues...) number for the specific pesticide of interest provided in the table in Unit III.A., by one of...

  4. 76 FR 60822 - Registration Review; Pesticide Dockets Opened for Review and Comment and Other Docket Actions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-30

    ...EPA has established registration review dockets for the pesticides listed in the table in Unit III.A. With this document, EPA is opening the public comment period for these registration reviews. Registration review is EPA's periodic review of pesticide registrations to ensure that each pesticide continues to satisfy the statutory standard for registration, that is, the pesticide can perform......

  5. Expression Pattern of Id Proteins in Medulloblastoma

    PubMed Central

    Snyder, Andrew D.; Dulin-Smith, Ashley N.; Houston, Ronald H.; Durban, Ashley N.; Brisbin, Bethany J.; Oostra, Tyler D.; Marshall, Jordan T.; Kahwash, Basil M.

    2013-01-01

    Inhibitor of DNA binding or inhibitor of differentiation (Id) proteins are up regulated in a variety of neoplasms, particularly in association with high-grade, poorly differentiated tumors, while differentiated tissues show little or no Id expression. The four Id genes are members of the helix-loop-helix (HLH) family of transcription factors and act as negative regulators of transcription by binding to and sequestering HLH complexes. We tested the hypothesis that Id proteins are overexpressed in medulloblastoma by performing immunohistochemistry using a medulloblastoma tissue microarray with 45 unique medulloblastoma and 11 normal control cerebella, and antibodies specific for Id1, Id2, Id3, and Id4. A semi-quantitative staining score that took staining intensity and the proportion of immunoreactive cells into account was used. Id1 was not detected in normal cerebella or in medulloblastoma cells, but 78 % of tumors showed strong Id1 expression in endothelial nuclei of tumor vessels. Id2 expression was scant in normal cerebella and increased in medulloblastoma (median staining score: 4). Id3 expression was noted in some neurons of the developing cerebellar cortex, but it was markedly up regulated in medulloblastoma (median staining score: 12) and in tumor endothelial cells. Id4 was not expressed in normal cerebella or in tumor cells. Id2 or Id3 overexpression drove proliferation in medulloblastoma cell lines by altering the expression of critical cell cycle regulatory proteins in favor of cell proliferation. This study shows that Id1 expression in endothelial cells may contribute to angiogenic processes and that increased expression of Id2 and Id3 in medulloblastoma is potentially involved in tumor cell proliferation and survival. PMID:23397264

  6. 10 CFR 2.815 - Docketing and acceptance review.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Docketing and acceptance review. 2.815 Section 2.815 Energy NUCLEAR REGULATORY COMMISSION RULES OF PRACTICE FOR DOMESTIC LICENSING PROCEEDINGS AND ISSUANCE OF ORDERS Rulemaking § 2.815 Docketing and acceptance review. (a) Each application for a standard...

  7. 7 CFR 1781.17 - Docket preparation and processing.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... water and irrigation. (6) Estimates of right-of-way Costs. The docket should include, as part of the... needed. (2) Instructions for preparation of docket. When the Rural Development State Director has... State Conservationist concurring in the feasibility of the WS work of improvement or RCD measure...

  8. 33 CFR 1.05-25 - Public docket.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... docket. Public dockets for Coast Guard rulemakings are available electronically at http://www.regulations... http://www.regulations.gov. Paragraph (a) of this section describes how to access and view these... available for inspection at the appropriate Captains of the Port Office or online at...

  9. 39 CFR 3001.13 - Docket and hearing calendar.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 39 Postal Service 1 2010-07-01 2010-07-01 false Docket and hearing calendar. 3001.13 Section 3001.13 Postal Service POSTAL REGULATORY COMMISSION PERSONNEL RULES OF PRACTICE AND PROCEDURE Rules of... remotely via the Commission's Web site, or viewed at the Commission's docket section during...

  10. 48 CFR 6302.3 - Docketing of appeals (Rule 3).

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 7 2013-10-01 2012-10-01 true Docketing of appeals (Rule 3). 6302.3 Section 6302.3 Federal Acquisition Regulations System DEPARTMENT OF TRANSPORTATION BOARD OF CONTRACT APPEALS RULES OF PROCEDURE 6302.3 Docketing of appeals (Rule 3). Following receipt by...

  11. 78 FR 773 - Hartford Financial Services Group, Inc., Commercial/Actuarial/Information Delivery Services (IDS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-04

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Employment and Training Administration Hartford Financial Services Group, Inc., Commercial/Actuarial/ Information Delivery Services (IDS)/Corporate & Financial Reporting Group, Hartford, CT; Notice of...

  12. IDS contribution to ITRF2008

    NASA Astrophysics Data System (ADS)

    Valette, Jean-Jacques; Lemoine, Frank G.; Ferrage, Pascale; Yaya, Philippe; Altamimi, Zuheir; Willis, Pascal; Soudarin, Laurent

    2010-12-01

    For the first time, the International DORIS Service (IDS) has produced a technique level combination based on the contributions of seven analysis centers (ACs), including the European Space Operations Center (ESOC), Geodetic Observatory Pecny (GOP), Geoscience Australia (GAU), the NASA Goddard Space Flight Center (GSFC), the Institut Géographique National (IGN), the Institute of Astronomy, Russian Academy of Sciences (INASAN, named as INA), and CNES/CLS (named as LCA). The ACs used five different software packages to process the DORIS data from 1992 to 2008, including NAPEOS (ESA), Bernese (GOP), GEODYN (GAU, GSC), GIPSY/OASIS (INA), and GINS (LCA). The data from seven DORIS satellites, TOPEX/Poseidon, SPOT-2, SPOT-3, SPOT-4, SPOT-5, Envisat and Jason-1 were processed and all the analysis centers produced weekly SINEX files in either variance-covariance or normal equation format. The processing by the analysis centers used the latest GRACE-derived gravity models, forward modelling of atmospheric gravity, updates to the radiation pressure modelling to improve the DORIS geocenter solutions, denser parameterization of empirically determined drag coefficients to improve station and EOP solutions, especially near the solar maximum in 2001-2002, updated troposphere mapping functions, and an ITRF2005-derived station set for orbit determination, DPOD2005. The CATREF software was used to process the weekly AC solutions, and produce three iterations of an IDS global weekly combination. Between the development of the initial solution IDS-1, and the final solution, IDS-3, the ACs improved their analysis strategies and submitted updated solutions to eliminate troposphere-derived biases in the solution scale, to reduce drag-related degradations in station positioning, and to refine the estimation strategy to improve the combination geocenter solution. An analysis of the frequency content of the individual AC geocenter and scale solutions was used as the basis to define the

  13. IDS contribution to ITRF2013

    NASA Astrophysics Data System (ADS)

    Moreaux, Guilhem; Lemoine, Frank; Willis, Pascal; Capdeville, Hugues; Otten, Michiel; Stepanek, Petr; Kuzin, Sergei; Ferrage, Pascale

    2014-05-01

    In the context of the forthcoming ITRF 2013, the IDS Combination Center is involved in the estimation of DORIS stations positions/velocities as well as Earth orientation parameters from DORIS data. These computations are based on the latest series of all of the 6 IDS Analysis Centers multi-satellite weekly SINEX solutions from January 1993 to December 2013. The primary objective of this study is to analyze the DORIS contribution to ITRF2013 in terms of (1) geocenter and scale solutions; (2) stations positions. Furthermore, we will focus on the impact of new standards such as the application of DORIS ground antennas phase laws in the data processing and improved modeling of DORIS ground beacon frequency variations. We will also address benefits of including new DORIS data in the IDS combination compared to ITRF2008, including data from new DORIS missions (e.g. Jason-2, Cryosat-2), as well as improved data for both Jason-1 and SPOT-5 where these satellite data are now corrected to accommodate perturbations introduced by the South Atlantic Anomaly (SAA). In addition we will compare this new DORIS ITRF realization to the previous realization (ITRF2008).

  14. 75 FR 62810 - Twenty-Fourth Update of the Federal Agency Hazardous Waste Compliance Docket

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-13

    ... facilities not previously listed on the Docket and reported to EPA since the last update of the Docket (73 FR... facilities to be included on the Docket was published in the Federal Register on February 12, 1988 (53 FR 4280). Since then, updates to the Docket have been published on November 16, 1988 (54 FR...

  15. 1. View looking northeast, showing from left, ID65D, ID65B and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. View looking northeast, showing from left, ID-65-D, ID-65-B and ID-65-A from the location of the former driveway. - Buffalo Guard Station, U.S. Highway 20/191 at Buffalo River, Island Park, Fremont County, ID

  16. The International Doris Service (IDS)

    NASA Astrophysics Data System (ADS)

    Tavernier, G.; Feissel-Vernier, M.; Lemoine, F.; Noll, C.; Ries, J.; Soudarin, L.; Willis, P.

    The DORIS system was developed for precise orbit determination and precise positioning on Earth. In continuation of the DORIS Pilot Experiment initiated in 1999, the International DORIS Service (IDS) officially started on July 1, 2003 as an IAG Service after the decision of the IAG Executive Committee at the IUGG General Assembly in Sapporo. Following this decision, the IERS Directing board accepted the DORIS Service as a new IERS external service. Six satellites fitted out with DORIS receivers are currently flying, permanently observed by 56 well distributed stations. Among them, three satellites equipped with new DORIS receivers (Jason-1, ENVISAT and SPOT5) have been successfully launched between Dec. 2001 and May 2002. All of them host a navigation function, called DIODE. Two new stations have been added to the permanent network in 2003: Crozet (French Southern Indian Ocean territories) and Jiufeng (China). In response to proposals submitted by candidate host agencies in the frame of IDS, the following stations were installed: Wettzell (Germany), Gavdos (radar altimeter calibration site in Crete), Lambert and Sorsdal (Glacier movement monitoring in Antarctica) in Winter 2001/2002, 2002/2003 and 2003/2004. The 2003 and early 2004 highlights include the renovation of a number of stations, reorganization of the Data Centers, and new developments in the coordination of analyses. While the Analysis Centers continue their support to IERS, the Analysis Coordinator and the Central Bureau are jointly developing comparison and combination activities that eventually aim at benefiting to the IERS Combination Pilot Project.

  17. 10 CFR 2.815 - Docketing and acceptance review.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... ORDERS Rulemaking § 2.815 Docketing and acceptance review. (a) Each application for a standard design... copy of the tendered application will be available for public inspection at the NRC Web site,...

  18. 78 FR 61442 - Idaho Disaster #ID-00027 Declaration of Economic Injury

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-03

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Idaho Disaster ID-00027 Declaration of Economic Injury AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Economic Injury Disaster Loan (EIDL) declaration for...

  19. 7 CFR 15f.8 - What does the Docketing Clerk do with my Section 741 Complaint Request?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... My Complaint Informally With OCR? § 15f.8 What does the Docketing Clerk do with my Section 741 Complaint Request? All Section 741 Complaint Requests docketed by the OCR Docketing Clerk will be...

  20. 7 CFR 15f.8 - What does the Docketing Clerk do with my Section 741 Complaint Request?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... My Complaint Informally With OCR? § 15f.8 What does the Docketing Clerk do with my Section 741 Complaint Request? All Section 741 Complaint Requests docketed by the OCR Docketing Clerk will be...

  1. 7 CFR 15f.8 - What does the Docketing Clerk do with my Section 741 Complaint Request?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... My Complaint Informally With OCR? § 15f.8 What does the Docketing Clerk do with my Section 741 Complaint Request? All Section 741 Complaint Requests docketed by the OCR Docketing Clerk will be...

  2. 7 CFR 15f.8 - What does the Docketing Clerk do with my Section 741 Complaint Request?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... My Complaint Informally With OCR? § 15f.8 What does the Docketing Clerk do with my Section 741 Complaint Request? All Section 741 Complaint Requests docketed by the OCR Docketing Clerk will be...

  3. 7 CFR 15f.8 - What does the Docketing Clerk do with my Section 741 Complaint Request?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... My Complaint Informally With OCR? § 15f.8 What does the Docketing Clerk do with my Section 741 Complaint Request? All Section 741 Complaint Requests docketed by the OCR Docketing Clerk will be...

  4. Towards an operational IDS combination

    NASA Astrophysics Data System (ADS)

    Valette, J.; Soudarin, L.; Willis, P.; Govind, R.; Stepanek, P.; Lemoine, F.; Altamimi, Z.; Le Bail, K.

    2007-12-01

    The International DORIS Service is in operation since 2003. Over the last four years, five instruments on the SPOT 2-4-5, ENVISAT and the JASON-1 satellites have provided DORIS Doppler data collected from a global network of about 50 stations. The number of Analysis Centers (AC) who have processed the data and have high level experience has progressively risen. Among them, two ACs : IGN/JPL using GYPSY/OASIS software and LEGOS/CLS using GINS/DYNAMO software now provide solutions of station coordinates and EOPs on a routine basis to the IGN and NASA CDDIS data centers. INASAN also processes DORIS data using GYPSY/OASIS software and submitted SINEX files to the ITRF2005 solution. The two newest DORIS analysis centers, include Geoscience Australia (GA) using the NASA GEODYN software and the Geodetic Observatory Pecny (GOP) using the BERNESE software. The GOP has adapted software not originally designed to process DORIS data. The performance reached by the new analysis centers in orbit and station positions determination is very encouraging. The availability of geodetic solutions from different algorithms and software packages allows us to efficiently contribute to cross-comparison of the solutions and to the improvement of the DORIS technique. The operational combinations of SINEX solutions contributed by the DORIS analysis centers will have to perform at least two functions. The first one is an internal validation of each individual AC time series. The second is a combination of all the AC weekly solutions in order to generate IDS combined products for station coordinates and EOPs. The paper presents the status of each AC processing (strategy, results, organization and future plan) and the combinations of solutions for the period of several years (2002-2006 as an objective). The combinations are realized with the IGN/LAREG CATREF software. Essential to the analysts, the station network event file (discontinuities, poor data span) will be updated and listed. The

  5. Training for Instructional Design: Learning I.D. through the I.D. Process.

    ERIC Educational Resources Information Center

    Kemp, Jerrold E.

    1986-01-01

    Examines instructional design (ID) courses offered in instructional technology masters program at San Jose State University. Course objectives, participants' needs assessment, resources and study methods, and student evaluation are discussed for introductory ID course; learning activities of advanced ID seminar are outlined. Sample student…

  6. Negotiating roadblocks to IDS-physician equity joint ventures.

    PubMed

    Dubow, S F; Benoff, M

    1998-09-01

    Integrated delivery systems (IDSs) may find that forming an equity joint venture relationship with a physician group practice is the best way to integrate physicians into their networks. IDSs have a choice between two basic equity structures: affiliated group practice, in which a management services organization (MSO) handles all practice management infrastructure and the physician group is a physician-only organization; and integrated group practice, in which the physician group encompasses both the physician practice and the administrative infrastructure. The choice of equity structure and how it should be implemented hinge on several legal issues, including the existence of a corporate-practice-of-medicine statute in the IDS's state, compliance with the Federal antikickback statute and Stark laws, and various issues regarding the IDS's tax-exempt status. IDSs also should consider pragmatic issues, particularly those associated with aligning the economic incentives of the two partners. PMID:10182740

  7. R&D100: IC ID

    SciTech Connect

    Hamlet, Jason; Pierson, Lyndon; Bauer, Todd

    2015-11-19

    Supply chain security to detect, deter, and prevent the counterfeiting of networked and stand-alone integrated circuits (ICs) is critical to cyber security. Sandia National Laboratory researchers have developed IC ID to leverage Physically Unclonable Functions (PUFs) and strong cryptographic authentication to create a unique fingerprint for each integrated circuit. IC ID assures the authenticity of ICs to prevent tampering or malicious substitution.

  8. 75 FR 16345 - Administrative Practices and Procedures; Good Guidance Practices; Technical Amendment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-01

    ... HUMAN SERVICES Food and Drug Administration 21 CFR Part 10 (formerly Docket No. 1999N-4783) Administrative Practices and Procedures; Good Guidance Practices; Technical Amendment AGENCY: Food and Drug Administration, HHS. ACTION: Final rule; technical amendment. SUMMARY: The Food and Drug Administration (FDA)...

  9. 78 FR 44857 - Seventy-Second Report of the TSCA Interagency Testing Committee to the Administrator of the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-24

    ...) rule that was published in the Federal Register of December 3, 2012 (77 FR 71561) (FRL-9355-9). After... Federal Register of December 28, 2012 (77 FR 76419) (FRL-9375-3) due to questions and concerns raised... FR 4188, February 2, 1996) (FRL-4991-6). Available at www.regulations.gov . Docket ID number...

  10. 75 FR 34960 - Import Administration IA ACCESS Pilot Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ... ACCESS Pilot Program in the Federal Register at 75 FR 32341. The reference to the Docket No. ITA- 2010.... The Department publishes this notice to correct this number. Accordingly, in FR Doc. 2010-13733, at... International Trade Administration 19 CFR Part 351 Import Administration IA ACCESS Pilot Program AGENCY:...

  11. 75 FR 21000 - Draft Guidance for the Public, Food and Drug Administration Advisory Committee Members, and Food...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-22

    ... HUMAN SERVICES Food and Drug Administration (formerly Docket No. 02D-0049) Draft Guidance for the Public, Food and Drug Administration Advisory Committee Members, and Food and Drug Administration Staff: Public...: Food and Drug Administration, HHS. ACTION: Notice. SUMMARY: The Food and Drug Administration (FDA)...

  12. 29 CFR 501.38 - Notice of docketing.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 3 2011-07-01 2011-07-01 false Notice of docketing. 501.38 Section 501.38 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR REGULATIONS ENFORCEMENT OF CONTRACTUAL OBLIGATIONS FOR TEMPORARY ALIEN AGRICULTURAL WORKERS ADMITTED UNDER SECTION 218 OF...

  13. 29 CFR 501.38 - Notice of docketing.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Notice of docketing. 501.38 Section 501.38 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR REGULATIONS ENFORCEMENT OF CONTRACTUAL OBLIGATIONS FOR TEMPORARY ALIEN AGRICULTURAL WORKERS ADMITTED UNDER SECTION 218 OF...

  14. 43 CFR 4.103 - Forwarding and docketing of appeals.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... mailing (or the date of receipt, if the notice was otherwise conveyed) and within 5 days shall forward said notice of appeal to the Board by certified mail. He shall also promptly notify the Department's... the fact of docketing, together with a copy of these rules, shall be mailed promptly by certified...

  15. 20 CFR 802.302 - Docketing of appeals.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... order upon the date on which a notice of appeal is received. Correspondence or further applications in...; publication of decision. The docket of the Board shall be open to public inspection. The Board shall publish its decisions in a form which is readily available for inspection, and shall allow the public...

  16. 20 CFR 802.302 - Docketing of appeals.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... order upon the date on which a notice of appeal is received. Correspondence or further applications in...; publication of decision. The docket of the Board shall be open to public inspection. The Board shall publish its decisions in a form which is readily available for inspection, and shall allow the public...

  17. 20 CFR 802.302 - Docketing of appeals.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... order upon the date on which a notice of appeal is received. Correspondence or further applications in...; publication of decision. The docket of the Board shall be open to public inspection. The Board shall publish its decisions in a form which is readily available for inspection, and shall allow the public...

  18. 47 CFR 1.730 - The Enforcement Bureau's Accelerated Docket.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... which raise issues that may not be decided on delegated authority (see 47 U.S.C. 155(c)(1); 47 CFR 0.291... 47 Telecommunication 1 2012-10-01 2012-10-01 false The Enforcement Bureau's Accelerated Docket. 1.730 Section 1.730 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL PRACTICE AND...

  19. 47 CFR 1.730 - The Enforcement Bureau's Accelerated Docket.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... which raise issues that may not be decided on delegated authority (see 47 U.S.C. 155(c)(1); 47 CFR 0.291... 47 Telecommunication 1 2014-10-01 2014-10-01 false The Enforcement Bureau's Accelerated Docket. 1.730 Section 1.730 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL PRACTICE AND...

  20. 47 CFR 1.730 - The Enforcement Bureau's Accelerated Docket.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... which raise issues that may not be decided on delegated authority (see 47 U.S.C. 155(c)(1); 47 CFR 0.291... 47 Telecommunication 1 2013-10-01 2013-10-01 false The Enforcement Bureau's Accelerated Docket. 1.730 Section 1.730 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL PRACTICE AND...

  1. 47 CFR 1.730 - The Enforcement Bureau's Accelerated Docket.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... which raise issues that may not be decided on delegated authority (see 47 U.S.C. 155(c)(1); 47 CFR 0.291... 47 Telecommunication 1 2011-10-01 2011-10-01 false The Enforcement Bureau's Accelerated Docket. 1.730 Section 1.730 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL PRACTICE AND...

  2. 39 CFR 3020.53 - Docket and notice.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    .... The Commission will establish a docket for each request to modify the market dominant list or the competitive product list, promptly publish notice of the request in the Federal Register, and post the filing on its Web site. The notice shall include: (a) The general nature of the proceeding; (b) A...

  3. 39 CFR 3020.33 - Docket and notice.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... The Commission will establish a docket for each request to modify the market dominant list or the competitive product list, promptly publish notice of the request in the Federal Register, and post the filing on its Web site. The notice shall include: (a) The general nature of the proceeding; (b) A...

  4. 39 CFR 3020.73 - Docket and notice.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Commission will establish a docket for each request to modify the market dominant list or the competitive product list, promptly publish notice of the request in the Federal Register, and post the filing on its Web site. The notice shall include: (a) The general nature of the proceeding; (b) A reference to...

  5. 39 CFR 3020.33 - Docket and notice.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    .... The Commission will establish a docket for each request to modify the market dominant list or the competitive product list, promptly publish notice of the request in the Federal Register, and post the filing on its Web site. The notice shall include: (a) The general nature of the proceeding; (b) A...

  6. 39 CFR 3020.73 - Docket and notice.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Commission will establish a docket for each request to modify the market dominant list or the competitive product list, promptly publish notice of the request in the Federal Register, and post the filing on its Web site. The notice shall include: (a) The general nature of the proceeding; (b) A reference to...

  7. 39 CFR 3020.73 - Docket and notice.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Commission will establish a docket for each request to modify the market dominant list or the competitive product list, promptly publish notice of the request in the Federal Register, and post the filing on its Web site. The notice shall include: (a) The general nature of the proceeding; (b) A reference to...

  8. 39 CFR 3020.33 - Docket and notice.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    .... The Commission will establish a docket for each request to modify the market dominant list or the competitive product list, promptly publish notice of the request in the Federal Register, and post the filing on its Web site. The notice shall include: (a) The general nature of the proceeding; (b) A...

  9. 39 CFR 3020.53 - Docket and notice.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    .... The Commission will establish a docket for each request to modify the market dominant list or the competitive product list, promptly publish notice of the request in the Federal Register, and post the filing on its Web site. The notice shall include: (a) The general nature of the proceeding; (b) A...

  10. 39 CFR 3020.53 - Docket and notice.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... The Commission will establish a docket for each request to modify the market dominant list or the competitive product list, promptly publish notice of the request in the Federal Register, and post the filing on its Web site. The notice shall include: (a) The general nature of the proceeding; (b) A...

  11. 39 CFR 3020.33 - Docket and notice.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .... The Commission will establish a docket for each request to modify the market dominant list or the competitive product list, promptly publish notice of the request in the Federal Register, and post the filing on its Web site. The notice shall include: (a) The general nature of the proceeding; (b) A...

  12. 39 CFR 3020.73 - Docket and notice.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Commission will establish a docket for each request to modify the market dominant list or the competitive product list, promptly publish notice of the request in the Federal Register, and post the filing on its Web site. The notice shall include: (a) The general nature of the proceeding; (b) A reference to...

  13. 39 CFR 3020.53 - Docket and notice.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .... The Commission will establish a docket for each request to modify the market dominant list or the competitive product list, promptly publish notice of the request in the Federal Register, and post the filing on its Web site. The notice shall include: (a) The general nature of the proceeding; (b) A...

  14. 39 CFR 3020.53 - Docket and notice.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Service POSTAL REGULATORY COMMISSION PERSONNEL PRODUCT LISTS Requests Initiated by Users of the Mail To Modify the Product Lists Described Within the Mail Classification Schedule § 3020.53 Docket and notice... competitive product list, promptly publish notice of the request in the Federal Register, and post the...

  15. 39 CFR 3020.33 - Docket and notice.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Service POSTAL REGULATORY COMMISSION PERSONNEL PRODUCT LISTS Requests Initiated by the Postal Service To Modify the Product Lists Described Within the Mail Classification Schedule § 3020.33 Docket and notice... competitive product list, promptly publish notice of the request in the Federal Register, and post the...

  16. 39 CFR 3020.73 - Docket and notice.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Service POSTAL REGULATORY COMMISSION PERSONNEL PRODUCT LISTS Proposal of the Commission To Modify the Product Lists Described Within the Mail Classification Schedule § 3020.73 Docket and notice. The... product list, promptly publish notice of the request in the Federal Register, and post the filing on...

  17. Id-1 and Id-2 genes and products as markers of epithelial cancer

    DOEpatents

    Desprez, Pierre-Yves; Campisi, Judith

    2011-10-04

    A method for detection and prognosis of breast cancer and other types of cancer. The method comprises detecting expression, if any, for both an Id-1 and an Id-2 genes, or the ratio thereof, of gene products in samples of breast tissue obtained from a patient. When expressed, Id-1 gene is a prognostic indicator that breast cancer cells are invasive and metastatic, whereas Id-2 gene is a prognostic indicator that breast cancer cells are localized and noninvasive in the breast tissue.

  18. Id-1 and Id-2 genes and products as markers of epithelial cancer

    DOEpatents

    Desprez, Pierre-Yves; Campisi, Judith

    2008-09-30

    A method for detection and prognosis of breast cancer and other types of cancer. The method comprises detecting expression, if any, for both an Id-1 and an Id-2 genes, or the ratio thereof, of gene products in samples of breast tissue obtained from a patient. When expressed, Id-1 gene is a prognostic indicator that breast cancer cells are invasive and metastatic, whereas Id-2 gene is a prognostic indicator that breast cancer cells are localized and noninvasive in the breast tissue.

  19. Combined Id1 and Id3 Deletion Leads to Severe Erythropoietic Disturbances

    PubMed Central

    Zhao, Qingshi; Chang, Corey; Gonzalez, J. Patrick; Alzahrani, Kamal; Button, Jessica L; Fraidenraich, Diego

    2016-01-01

    The Inhibitor of DNA Binding (Id) proteins play a crucial role in regulating hematopoiesis and are known to interact with E proteins and the bHLH family of transcription factors. Current efforts seek to elucidate the individual roles of Id members in regulating hematopoietic development and specification. However, the nature of their functional redundancies remains elusive since ablation of multiple Id genes is embryonically lethal. We developed a model to test this compensation in the adult. We report that global Id3 ablation with Tie2Cre-mediated conditional ablation of Id1 in both hematopoietic and endothelial cells (Id cDKO) extends viability to 1 year but leads to multi-lineage hematopoietic defects including the emergence of anemia associated with defective erythroid development, a novel phenotype unreported in prior single Id knockout studies. We observe decreased cell counts in the bone marrow and splenomegaly to dimensions beyond what is seen in single Id knockout models. Transcriptional dysregulation of hematopoietic regulators observed in bone marrow cells is also magnified in the spleen. E47 protein levels were elevated in Id cDKO bone marrow cell isolates, but decreased in the erythroid lineage. Chromatin immunoprecipitation (ChIP) studies reveal increased occupancy of E47 and GATA1 at the promoter regions of β-globin and E2A. Bone marrow transplantation studies highlight the importance of intrinsic Id signals in maintaining hematopoietic homeostasis while revealing a strong extrinsic influence in the development of anemia. Together, these findings demonstrate that loss of Id compensation leads to dysregulation of the hematopoietic transcriptional network and multiple defects in erythropoietic development in adult mice. PMID:27128622

  20. 78 FR 42381 - Administrative Detention of Drugs Intended for Human or Animal Use

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-15

    ... regulations for the administrative detention of drugs (78 FR 21085). The docket was intended to ensure that... July 15, 2013 Part IV Department of Health and Human Services Food and Drug Administration 21 CFR Parts... / Proposed Rules#0;#0; ] DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration 21 CFR Parts...

  1. 77 FR 6026 - Proposed Amendment of Class E Airspace; Boise, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-07

    ... Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of.... 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 2. The incorporation by reference in 14 CFR... Federal Aviation Administration 14 CFR Part 71 Proposed Amendment of Class E Airspace; Boise, ID...

  2. 75 FR 41773 - Proposed Amendment of Class E Airspace; Arco, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ... ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does..., 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 2. The incorporation by... Federal Aviation Administration 14 CFR Part 71 Proposed Amendment of Class E Airspace; Arco, ID...

  3. 75 FR 11936 - Hewlett Packard; Technical Support Call Center; Boise, ID; Notice of Termination of Investigation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Employment and Training Administration Hewlett Packard; Technical Support Call Center; Boise, ID; Notice of Termination of Investigation Pursuant to Section 221 of the Trade Act of 1974, as amended, an...

  4. 76 FR 8324 - Proposed Amendment of Class E Airspace; McCall, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-14

    ... Executive Order 12866; (2) is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR... follows: Authority: 49 U.S.C. 106(g), 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp... Federal Aviation Administration 14 CFR Part 71 Proposed Amendment of Class E Airspace; McCall, ID...

  5. 75 FR 15741 - Applied Materials; Boise, ID; Notice of Termination of Investigation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-30

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Employment and Training Administration Applied Materials; Boise, ID; Notice of Termination of Investigation Pursuant to Section 221 of the Trade Act of 1974, as amended, an investigation was initiated in response...

  6. 76 FR 9266 - Proposed Amendment of Class D and Class E Airspace; Idaho Falls, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-17

    ...'' under DOT Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant.... 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 2. The incorporation by reference in 14 CFR... Falls, ID AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of proposed...

  7. Evaluation of docket files for terminated source material licenses

    SciTech Connect

    Holoway, C.F.; Lantz, P.M.; Dickson, H.W.

    1980-01-01

    Terminated source material licenses from docket files of the Nuclear Regulatory Commission (NRC) have been evaluated with respect to the potential for residual radiological health problems. Some 8,275 source material docket files were sent from the Federal Repository for NRC to Oak Ridge National Laboratory for evaluation. An NRC printed inventory of terminated source material licenses was sent separately and input to a computer file for ready access. The files were inventoried and a methodology was developed for evaluation of these files. The methodology included development of a combined analysis/computer input form. Pertinent data were abstracted from each file, placed on this form, and entered into a separate private-access computer file. At the same time, analysts using screening criteria made a preliminary categorization of the files. All files categorized initially as potential radiological health problems were reviewed in depth to arrive at a final categorization. Criteria for judgment included quantities of source material (uranium and/or thorium) going to and leaving the site in question during the operational lifetime of the site, disposition of source material not leaving the site, types of operations carried on at the site during the licensed period, and the physicochemical forms of products and wastes involved. In the final analysis, 193 dockets were identified as having potential for residual radiological health problems.

  8. What Is "Real-World" ID Anyway?

    ERIC Educational Resources Information Center

    Hardre, Patricia L.

    2013-01-01

    The term "real-world ID" is commonly heard at professional conferences, but its meaning is assumed rather than defined. Unless we examine the meanings and implications of rhetoric of the field, we risk error in presenting ourselves, and we risk derailing the progress of our profession. This article examines the term "real world" as applied to…

  9. 76 FR 62387 - Public User ID Badging

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-07

    ... Search Facility, United States Patent and Trademark Office, P.O. Box 1450, Alexandria, VA 22313-1450; by...)(1) to maintain a Public Search Facility to provide patent and trademark collections for searching... United States Patent and Trademark Office Public User ID Badging ACTION: Proposed collection;...

  10. Ids and ITRF2013: Contribution and Evaluation

    NASA Astrophysics Data System (ADS)

    Moreaux, G.; Lemoine, F. G.; Willis, P.; Capdeville, H.; Stepanek, P.; Otten, M.; Kuzin, S.; Ferrage, P.

    2014-12-01

    In the context of ITRF 2013, the IDS Combination Center is involved in the estimation of DORIS stations positions/velocities as well as Earth orientation parameters from DORIS data. These computations are based on the latest series of all of the six IDS Analysis Centers multi-satellite weekly SINEX solutions from January 1993 to December 2013. The primary objective of this study is to analyze the DORIS contribution to ITRF2013 in terms of (1) geocenter and scale solutions; (2) station positions and week-to-week repeatability; (3) EOPs. Comparisons with IDS contribution to ITRF2008 will address the benefits of including both new DORIS data, and new DORIS missions (e.g. Jason-1, Jason-2, Cryosat-2) in the IDS combination. These comparisons will also emphasize the application of the DORIS ground antennae phase laws in the data processing and the improved modeling of DORIS ground beacon frequency variations. In addition we will present early results of evaluation of ITRF2013 in the context of DORIS processing.

  11. Id-1, Id-2, and Id-3 co-expression correlates with prognosis in stage I and II lung adenocarcinoma patients treated with surgery and adjuvant chemotherapy.

    PubMed

    Antonângelo, Leila; Tuma, Taila; Fabro, Alexandre; Acencio, Milena; Terra, Ricardo; Parra, Edwin; Vargas, Francisco; Takagaki, Teresa; Capelozzi, Vera

    2016-06-01

    Inhibitors of DNA binding/inhibitors of differentiation (Id) protein family have been shown to be involved in carcinogenesis. However, the roles of Id during lung adenocarcinoma (ADC) progression remain unclear. Eighty-eight ADC samples were evaluated for Id-1,2,3 level and angiogenesis (CD 34 and VEGF microvessel density) by immunohistochemistry and morphometry. The impact of these markers was tested on follow-up until death or recurrence. A significant difference between tumor and normal tissue was found for Id-1,2,3 expression (P < 0.01). In addition, high levels of nuclear Id-1 were associated with higher angiogenesis in the tumor stroma (P < 0.01). Equally significant was the association between patients in T1-stage and low cytoplasmic Id-2, as well as patients in stage-IIb and low Id-3. High cytoplasm Id-3 expression was also directly associated to lymph nodes metastasis (P = 0.05). Patients at stages I to III, with low Id-1 and Id-3 cytoplasm histoscores showed significant long metastasis-free survival time than those with high Id-1 or Id-3 expression (P = 0.04). Furthermore, high MVD-CD34 and MVD-VEGF expression were associated with short recurrence-free survival compared to low MVD-CD34 and MVD-VEGF expressions (P = 0.04). Cox model analyses controlled for age, lymph node metastasis, and adjuvant treatments showed that nuclear Id-1, cytoplasmic Id-3, and MVD-CD34 were significantly associated with survival time. Median score for nuclear Id-1 and cytoplasmic Id-3 divided patients in two groups, being that those with increased Id-1 and Id-3 presented higher risk of death. Ids showed an independent prognostic value in patients with lung ADC, regardless of disease stage. Id-1 and Id-3 should be considered new target candidates in the development of personalized therapy in lung ADC. PMID:26869608

  12. Characterization of Inhibitor of differentiation (Id) proteins in human cornea.

    PubMed

    Mohan, Rajiv R; Morgan, Brandie R; Anumanthan, Govindaraj; Sharma, Ajay; Chaurasia, Shyam S; Rieger, Frank G

    2016-05-01

    Inhibitor of differentiation (Id) proteins are DNA-binding transcription factors involved in cellular proliferation, migration, inflammation, angiogenesis and fibrosis. However, their expression and role in the cornea is unknown. The present study was undertaken to characterize the expression of Id proteins and their interactions with the pro-fibrotic cytokine Transforming Growth Factor β1 (TGFβ1) and anti-fibrotic cytokine, bone morphogenic protein 7 (BMP7) in human cornea. Human donor corneas procured from Eye Bank were used. Id proteins were localized in human corneal sections using immunofluorescence. Primary cultures of human corneal fibroblasts (HCF) were established and treated with either TGFβ1 (5 ng/ml) or BMP7 (10 ng/ml) for 24 h in serum free medium. Expression of Id's in response to TGFβ1, BMP7 and TGFβ1 + BMP7 was analyzed by quantitative real time PCR (qRT-PCR) and western blot analysis. Id1 and Id2 proteins were ubiquitously expressed in the epithelial cells and stromal keratocytes in human cornea. The Id1 was localized to the basal epithelial cells as seen by immunohistochemistry. HCF expressed all known mammalian Id genes (Id1-Id4). In addition, Id1 and Id2 are selectively expressed in HCF. Treatment of human recombinant TGFβ1 (5 ng/ml) to serum-starved HCF showed a significant increase in Id genes (Id1, Id2 and Id4) at 2 h time point compared to BMP7 treatment, which showed time dependent increase in the expression of Id1-Id3 at 24-48 h. Combined treatment with TGFβ1 + BMP7 to HCF showed a significant increase in Id1 transcript and an increasing trend in Id3 and Id4 expression. The results of this study suggest that Id family of genes (Id1-Id4) are localized in the human cornea and expressed in the corneal fibroblasts. Also, Id's were differentially regulated with TGFβ1 and/or BMP7 in a time dependent manner and might serve as a therapeutic target in corneal fibrosis. PMID:26712606

  13. 10 CFR 52.163 - Administrative review of applications; hearings.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... FOR NUCLEAR POWER PLANTS Manufacturing Licenses § 52.163 Administrative review of applications... contained in 10 CFR part 2, including the requirements for docketing in § 2.101(a)(1) through (4) of this... hearings on manufacturing licenses are governed by the hearing procedures contained in 10 CFR part...

  14. 14 CFR 11.33 - How can I track FAA's rulemaking activities?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... docket number or the regulation identifier number. (a) Docket ID. We assign a docket ID to each... well as public comments on the proceeding, will display the same docket ID. This ID allows you...

  15. 14 CFR 11.33 - How can I track FAA's rulemaking activities?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... docket number or the regulation identifier number. (a) Docket ID. We assign a docket ID to each... well as public comments on the proceeding, will display the same docket ID. This ID allows you...

  16. 77 FR 64184 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel FIRE BELLE; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-18

    ... Maritime Administration Requested Administrative Waiver of the Coastwise Trade Laws: Vessel FIRE BELLE... this docket and will be available for inspection and copying at the above address between 10 a.m. and 5... of the vessel FIRE BELLE is: 6 pack sightseeing charters. Intended Commercial Use of...

  17. 78 FR 64284 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel ONE MORE; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-28

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF TRANSPORTATION Maritime Administration Requested Administrative Waiver of the Coastwise Trade Laws: Vessel ONE MORE... and all documents entered into this docket is available on the World Wide Web at...

  18. 40 CFR 300.805 - Location of the administrative record file.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... file. 300.805 Section 300.805 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... administrative record file. (a) The lead agency shall establish a docket at an office of the lead agency or other central location at which documents included in the administrative record file shall be located and a...

  19. 40 CFR 300.805 - Location of the administrative record file.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... file. 300.805 Section 300.805 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... administrative record file. (a) The lead agency shall establish a docket at an office of the lead agency or other central location at which documents included in the administrative record file shall be located and a...

  20. 77 FR 26821 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SUNCLIPPER II; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-07

    ... Maritime Administration Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SUNCLIPPER II... and all documents entered into this docket is available on the World Wide Web at http://www... the vessel SUNCLIPPER II is: Intended Commercial use of Vessel: ``6 passenger charter vessel for...

  1. 76 FR 76814 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel PATRIOT II; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-08

    ... Maritime Administration Requested Administrative Waiver of the Coastwise Trade Laws: Vessel PATRIOT II... and all documents entered into this docket is available on the World Wide Web at http://www... the vessel PATRIOT II is: Intended Commercial Use of Vessel: ``crewboat.'' Geographic...

  2. 77 FR 73735 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel PREDATOR II; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-11

    ... Maritime Administration Requested Administrative Waiver of the Coastwise Trade Laws: Vessel PREDATOR II... and all documents entered into this docket is available on the World Wide Web at http://www... of the vessel PREDATOR II is: Intended Commercial Use of Vessel: 6 pack private charters....

  3. 77 FR 35743 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SECOND CHANCE; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-14

    ... Maritime Administration Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SECOND CHANCE.... ADDRESSES: Comments should refer to docket number MARAD-2012-0067. Written comments may be submitted by hand... the vessel SECOND CHANCE is: Intended Commercial Use of Vessel: ``Small group charters.''...

  4. 78 FR 58603 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel ALL GOOD; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-24

    ... Maritime Administration Requested Administrative Waiver of the Coastwise Trade Laws: Vessel ALL GOOD... comments electronically via the Internet at http://www.regulations.gov . All comments will become part of... and all documents entered into this docket is available on the World Wide Web at...

  5. 33 CFR 148.207 - How and where may I view docketed documents?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Docket Management System Web site at http://www.dot.dms.gov. The projects are also listed by name and the assigned docket number at the G-PSO-5 Web site: http://www.uscg.mil/hq/g-m/mso/mso5.htm....

  6. 78 FR 60868 - Amendment of the Federal Docket Management System (EPA/GOV-2)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-02

    ... AGENCY Amendment of the Federal Docket Management System (EPA/GOV-2) AGENCY: Environmental Protection...-HQ- OEI-2012-0483 by one of the following methods: www.regulations.gov : Follow the online instructions for submitting comments. Email: oei.docket@epa.gov . Fax: 202-566-1752. Mail: OEI...

  7. 76 FR 18750 - Ocean Renewable Power Company, LLC; Notice of Change in Docket Number

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-05

    ... Energy Regulatory Commission Ocean Renewable Power Company, LLC; Notice of Change in Docket Number On July 24, 2009, Ocean Renewable Power Company, LLC (ORPC) filed a draft hydrokinetic pilot license... of this change in docket number, all current filings and issuances associated with the Eastport...

  8. 10 CFR 2.643 - Acceptance and docketing of application for limited work authorization.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Acceptance and docketing of application for limited work... Construct Certain Utilization Facilities; and Advance Issuance of Limited Work Authorizations Phased Applications Involving Limited Work Authorizations § 2.643 Acceptance and docketing of application for...

  9. 44 CFR 67.3 - Establishment and maintenance of a flood elevation determination docket (FEDD).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... of a flood elevation determination docket (FEDD). 67.3 Section 67.3 Emergency Management and... MITIGATION National Flood Insurance Program APPEALS FROM PROPOSED FLOOD ELEVATION DETERMINATIONS § 67.3 Establishment and maintenance of a flood elevation determination docket (FEDD). The Federal...

  10. 44 CFR 67.3 - Establishment and maintenance of a flood elevation determination docket (FEDD).

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... of a flood elevation determination docket (FEDD). 67.3 Section 67.3 Emergency Management and... MITIGATION National Flood Insurance Program APPEALS FROM PROPOSED FLOOD ELEVATION DETERMINATIONS § 67.3 Establishment and maintenance of a flood elevation determination docket (FEDD). The Federal...

  11. 44 CFR 67.3 - Establishment and maintenance of a flood elevation determination docket (FEDD).

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... of a flood elevation determination docket (FEDD). 67.3 Section 67.3 Emergency Management and... MITIGATION National Flood Insurance Program APPEALS FROM PROPOSED FLOOD ELEVATION DETERMINATIONS § 67.3 Establishment and maintenance of a flood elevation determination docket (FEDD). The Federal...

  12. 44 CFR 66.3 - Establishment of community case file and flood elevation study docket.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... case file and flood elevation study docket. 66.3 Section 66.3 Emergency Management and Assistance... National Flood Insurance Program CONSULTATION WITH LOCAL OFFICIALS § 66.3 Establishment of community case file and flood elevation study docket. (a) A file shall be established for each community at the...

  13. Docket Search. Part of a Planning Document for the Establishment of a Nationwide Educational Telecommunications System.

    ERIC Educational Resources Information Center

    Synergetics, Inc., Washington, DC.

    A docket search reviews selected educational telecommunications issues brought to the attention of the Federal Communications Commission (FCC). The docket search was part of an effort to develop a planning document for the establishment of a nationwide educational telecommunications system. Key policy-making proceedings were identified which dealt…

  14. 44 CFR 67.3 - Establishment and maintenance of a flood elevation determination docket (FEDD).

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... of a flood elevation determination docket (FEDD). 67.3 Section 67.3 Emergency Management and... MITIGATION National Flood Insurance Program APPEALS FROM PROPOSED FLOOD ELEVATION DETERMINATIONS § 67.3 Establishment and maintenance of a flood elevation determination docket (FEDD). The Federal...

  15. 44 CFR 66.3 - Establishment of community case file and flood elevation study docket.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... case file and flood elevation study docket. 66.3 Section 66.3 Emergency Management and Assistance... National Flood Insurance Program CONSULTATION WITH LOCAL OFFICIALS § 66.3 Establishment of community case file and flood elevation study docket. (a) A file shall be established for each community at the...

  16. Remembering One's ID/E-ntity : E/ID Protein Regulation of T Cell Memory

    PubMed Central

    Omilusik, Kyla D.; Shaw, Laura A.; Goldrath, Ananda W.

    2013-01-01

    Upon infection, CD8+ T cells proliferate and differentiate into armed effector cells capable of eliminating the assaulting pathogen. Although the majority of the antigen-specific T cells will die as the immune response wanes, a few will survive indefinitely to establish the memory population and provide long-lived protection against reinfection. E protein transcription factors and their inhibitors, ID proteins, operate to balance expression of genes that control CD8+ T cell differentiation through this process. Here, we discuss the role of ID2 and ID3 in promoting the generation and survival of effector and memory populations, particularly highlighting their reciprocal roles in shaping the CD8+ T cell response unique to the inflammatory milieu. We further examine this coordinated control of gene expression in the context of additional transcription factors within the transcriptional network that programs CD8+ effector and memory T cell differentiation. PMID:24094885

  17. Id transcriptional regulators in adipogenesis and adipose tissue metabolism

    PubMed Central

    Patil, Mallikarjun; Sharma, Bal Krishan; Satyanarayana, Ande

    2014-01-01

    Id proteins (Id1-Id4) are helix-loop-helix (HLH) transcriptional regulators that lack a basic DNA binding domain. They act as negative regulators of basic helixloop-helix (bHLH) transcription factors by forming heterodimers and inhibit their DNA binding and transcriptional activity. Id proteins are implicated in the regulation of various cellular mechanisms such as cell proliferation, cellular differentiation, cell fate determination, angiogenesis and tumorigenesis. A handful of recent studies also disclosed that Id proteins have critical functions in adipocyte differentiation and adipose tissue metabolism. Here, we reviewed the progress made thus far in understanding the specific functions of Id proteins in adipose tissue differentiation and metabolism. In addition to reviewing the known mechanisms of action, we also discuss possible additional mechanisms in which Id proteins might participate in regulating adipogenic and metabolic pathways. PMID:24896358

  18. Airless drying -- Developments since IDS'94

    SciTech Connect

    Stubbing, T.J.

    1999-09-01

    Since its introduction to IDS'94 delegates, significant progress has been made with the development of airless drying technology. The ceramic industry internationally is beginning to benefit from both the energy use and drying time reductions it achieves, while on the basis of further theoretical work carried out since 1993 other industries, including the bioenergy sector, should also soon begin to exploit its advantages. As global warming becomes a reality and oil reserves decline, superheated steam drying and gasification of biomass will contribute to the mitigation of those problems.

  19. Comparative Evaluation of the BD Phoenix Yeast ID Panel and Remel RapID Yeast Plus System for Yeast Identification

    PubMed Central

    Grant, Michelle L.; Parajuli, Shobha; Deleon-Gonsalves, Raquel; Potula, Raghava; Truant, Allan L.

    2016-01-01

    Becton Dickinson Phoenix Yeast ID Panel was compared to the Remel RapID Yeast Plus System using 150 recent clinical yeast isolates and the API 20C AUX system to resolve discrepant results. The concordance rate between the Yeast ID Panel and the RapID Yeast Plus System (without arbitration) was 93.3% with 97.3% (146/150) and 95.3% (143/150) of the isolates correctly identified by the Becton Dickinson Phoenix and the Remel RapID, respectively, with arbitration. PMID:27366167

  20. Comparative Evaluation of the BD Phoenix Yeast ID Panel and Remel RapID Yeast Plus System for Yeast Identification.

    PubMed

    Grant, Michelle L; Parajuli, Shobha; Deleon-Gonsalves, Raquel; Potula, Raghava; Truant, Allan L

    2016-01-01

    Becton Dickinson Phoenix Yeast ID Panel was compared to the Remel RapID Yeast Plus System using 150 recent clinical yeast isolates and the API 20C AUX system to resolve discrepant results. The concordance rate between the Yeast ID Panel and the RapID Yeast Plus System (without arbitration) was 93.3% with 97.3% (146/150) and 95.3% (143/150) of the isolates correctly identified by the Becton Dickinson Phoenix and the Remel RapID, respectively, with arbitration. PMID:27366167

  1. ID Image Characterization by Entropic Biometric Decomposition

    NASA Astrophysics Data System (ADS)

    Smoaca, Andreea; Coltuc, Daniela; Fournel, Thierry

    2011-03-01

    The paper proposes a statistical-based biometric decomposition for ID image recognition robust to a series of non malicious attacks generated by print/scan operations. Our goal is to label the single face expression by a signature, which is almost invariant to low filtering, noise addition and geometric attacks. The method is based on Independent Component Analysis (ICA) in a configuration which will allow a decomposition into some face characteristics. In this configuration known in literature as Architecture I, the most important coefficients issued from ICA are selected by looking for the independent components with maximum local entropy. A biometric label of fixed length is associated to any ID image to be enrolled, after projection on the learned basis, uniform quantization of the obtained coefficients and binary encoding. Two parameters were tuned: the number of quantization levels and the number of face characteristics. The latter one was modified, either by discarding coefficients after Principal Component Analysis in the beginning of FastICA algorithm, or by selecting the most prominent biometric features by applying an entropic criterion. The suggested method inherits the robustness of a global approach.

  2. To ID or Not to ID? Changes in Classification Rates of Intellectual Disability Using "DSM-5"

    ERIC Educational Resources Information Center

    Papazoglou, Aimilia; Jacobson, Lisa A.; McCabe, Marie; Kaufmann, Walter; Zabel, T. Andrew

    2014-01-01

    The "Diagnostic and Statistical Manual of Mental Disorders-Fifth Edition" ("DSM-5") diagnostic criteria for intellectual disability (ID) include a change to the definition of adaptive impairment. New criteria require impairment in one adaptive domain rather than two or more skill areas. The authors examined the diagnostic…

  3. Low Physical Fitness Levels in Older Adults with ID: Results of the HA-ID Study

    ERIC Educational Resources Information Center

    Hilgenkamp, Thessa I. M.; van Wijck, Ruud; Evenhuis, Heleen M.

    2012-01-01

    Physical fitness is as important to aging adults with ID as in the general population, but to date, the physical fitness levels of this group are unknown. Comfortable walking speed, muscle strength (grip strength), muscle endurance (30 s Chair stand) and cardiorespiratory endurance (10 m incremental shuttle walking test) were tested in a sample of…

  4. 77 FR 13074 - Opportunity for Designation in the Pocatello, ID; Evansville, IN; and Salt Lake City, UT Areas...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-05

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Grain Inspection, Packers and Stockyards Administration Opportunity for Designation in the Pocatello, ID; Evansville, IN; and Salt Lake City, UT Areas; Request for Comments on the Official Agencies Servicing...

  5. DriveID: safety innovation through individuation.

    PubMed

    Sawyer, Ben; Teo, Grace; Mouloua, Mustapha

    2012-01-01

    The driving task is highly complex and places considerable perceptual, physical and cognitive demands on the driver. As driving is fundamentally an information processing activity, distracted or impaired drivers have diminished safety margins compared with non- distracted drivers (Hancock and Parasuraman, 1992; TRB 1998 a & b). This competition for sensory and decision making capacities can lead to failures that cost lives. Some groups, teens and elderly drivers for example, have patterns of systematically poor perceptual, physical and cognitive performance while driving. Although there are technologies developed to aid these different drivers, these systems are often misused and underutilized. The DriveID project aims to design and develop a passive, automated face identification system capable of robustly identifying the driver of the vehicle, retrieve a stored profile, and intelligently prescribing specific accident prevention systems and driving environment customizations. PMID:22317376

  6. 77 FR 70166 - Provisions of the Food and Drug Administration Safety and Innovation Act Related to Medical Gases...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-23

    ...The Food and Drug Administration (FDA) is establishing a public docket for information pertaining to FDA's implementation of the provisions of the Food and Drug Administration Safety and Innovation Act (FDASIA) related to medical gases. This action is intended to ensure that information submitted to FDA on the implementation of the medical gas provisions of FDASIA is available to all......

  7. 77 FR 21150 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SIR MARTIN II; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-09

    ... Maritime Administration Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SIR MARTIN II... and all documents entered into this docket is available on the World Wide Web at http://www... the vessel SIR MARTIN II is: Intended Commercial Use of Vessel: ``Day charter up to 6 passengers...

  8. 78 FR 16824 - Tobacco Product Manufacturing Practice; Establishment of a Public Docket

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-19

    ... docket to obtain input on recommendations for regulations on good manufacturing practice for tobacco... current good manufacturing practice, or hazard analysis and critical control point methodology.'' On... Product Good Manufacturing Practices Regulation and Request for Meeting, submitted to FDA, January...

  9. The Role of Id2 Protein in Neuroblatoma in Children.

    PubMed

    Wieczorek, Aleksandra; Balwierz, Walentyna

    2015-09-01

    Id (DNA binding and/or differentiation) proteins occur physiologically during ontogenesis and negatively regulate the activity of other helix-loop-helix (HLH) proteins. Id2 protein causes block of cells differentiation in the S phase of the cell cycle and regulates the activity of Rb protein. The role of Id2 protein in physiological cell cycle progression and in neuroblastoma (NBL) pathogenesis was proposed by Lasorella. The aim of the study was evaluation of Id2 expression and its prognostic significance in NBL cells coming from primary tumors and evaluation of its prognostic significance, and correlation of Id2 expression with known prognostic factors. Sixty patients with primary NBL treated from 1991 to 2005 were included in the analysis. We found 50 patients with high and 10 patients with low intensity of Id2 expression. The median percentage of NBL cells with Id2 expression was 88 %. We found no correlation between the number of NBL cells or the intensity of Id2 expression and OS and DFS. In patients with stage 4 NBL, almost all patients had high expression of Id2 and it was significantly more common than in other disease stages (p = 0,03). We found no correlation between Id2 expression and other known prognostic factor in NBL patients. We assume that Id2 is not prognostic factor. However, due to its abundant expression in most of NBL cells and its role in cell cycle, it may be potential therapeutic target. Exact knowledge of expression time may be helpful in explaining mechanisms of oncogenesis. PMID:25771836

  10. An ID2-dependent mechanism for VHL inactivation in cancer.

    PubMed

    Lee, Sang Bae; Frattini, Veronique; Bansal, Mukesh; Castano, Angelica M; Sherman, Dan; Hutchinson, Keino; Bruce, Jeffrey N; Califano, Andrea; Liu, Guangchao; Cardozo, Timothy; Iavarone, Antonio; Lasorella, Anna

    2016-01-14

    Mechanisms that maintain cancer stem cells are crucial to tumour progression. The ID2 protein supports cancer hallmarks including the cancer stem cell state. HIFα transcription factors, most notably HIF2α (also known as EPAS1), are expressed in and required for maintenance of cancer stem cells (CSCs). However, the pathways that are engaged by ID2 or drive HIF2α accumulation in CSCs have remained unclear. Here we report that DYRK1A and DYRK1B kinases phosphorylate ID2 on threonine 27 (Thr27). Hypoxia downregulates this phosphorylation via inactivation of DYRK1A and DYRK1B. The activity of these kinases is stimulated in normoxia by the oxygen-sensing prolyl hydroxylase PHD1 (also known as EGLN2). ID2 binds to the VHL ubiquitin ligase complex, displaces VHL-associated Cullin 2, and impairs HIF2α ubiquitylation and degradation. Phosphorylation of Thr27 of ID2 by DYRK1 blocks ID2-VHL interaction and preserves HIF2α ubiquitylation. In glioblastoma, ID2 positively modulates HIF2α activity. Conversely, elevated expression of DYRK1 phosphorylates Thr27 of ID2, leading to HIF2α destabilization, loss of glioma stemness, inhibition of tumour growth, and a more favourable outcome for patients with glioblastoma. PMID:26735018

  11. Id4 Marks Spermatogonial Stem Cells in the Mouse Testis

    PubMed Central

    Sun, Feng; Xu, Qing; Zhao, Danfeng; Degui Chen, Charlie

    2015-01-01

    Mammalian spermatogenesis is a classic adult stems cell–dependent process, supported by the self-renewal and differentiation of spermatogonial stem cells (SSCs). However, the identification of SSCs and elucidation of their behaviors in undisturbed testis has long been a big challenge. Here, we generated a knock-in mouse model, Id4-2A-CreERT2-2A-tdTomato, which allowed us to mark Id4-expressing (Id4+) cells at different time points in situ and track their behaviors across distinct developmental stages during steady-state and regenerating spermatogenesis. We found that Id4+ cells continue to produce spermatogonia, spermatocytes and sperm in mouse testis, showing they are capable of self-renewal and have differentiation potential. Consistent with these findings, ablation of Id4+ cells in mice results in a loss of spermatogenesis. Furthermore, developmental fate mapping reveals that Id4+ SSCs originate from neonate Id4+ gonocytes. Therefore, our results indicate that Id4 marks spermatogonial stem cells in the mouse testis. PMID:26621350

  12. A Contextual Model for Identity Management (IdM) Interfaces

    ERIC Educational Resources Information Center

    Fuller, Nathaniel J.

    2014-01-01

    The usability of Identity Management (IdM) systems is highly dependent upon design that simplifies the processes of identification, authentication, and authorization. Recent findings reveal two critical problems that degrade IdM usability: (1) unfeasible techniques for managing various digital identifiers, and (2) ambiguous security interfaces.…

  13. ID Model Construction and Validation: A Multiple Intelligences Case

    ERIC Educational Resources Information Center

    Tracey, Monica W.; Richey, Rita C.

    2007-01-01

    This is a report of a developmental research study that aimed to construct and validate an instructional design (ID) model that incorporates the theory and practice of multiple intelligences (MI). The study consisted of three phases. In phase one, the theoretical foundations of multiple Intelligences and ID were examined to guide the development…

  14. Catalogue of bright IDS stars with extensive cross-identifications

    NASA Astrophysics Data System (ADS)

    Lipaeva, N. A.; Sementsov, V. N.; Malkov, O. Yu.

    A new catalogue of bright binary stars is presented. The catalogue1 includes bright IDS systems and bright spectroscopic binaries. Besides IDS data (coordinates, relative positions, magnitudes and spectral classification), the catalogue contains extensive cross-identification and comments for 27452 systems. The catalogue is complete to the 9th mag, but also contains stars down to about 16th mag.

  15. 76 FR 37661 - Notification of Anticipated Delay in Administrative Appeal Decisions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-28

    ...: ] HM-231 (Docket No. PHMSA-2006-25736) Hazardous Materials; Miscellaneous Packaging Amendments...) material conforming to 49 CFR 173.63(b) and Class 7 (radioactive) material conforming to 49 CFR 173.421... Pipeline and Hazardous Materials Safety Administration 49 CFR Parts 105, 107, 109, 171, 172, 173, 174,...

  16. 77 FR 11798 - Federal Housing Administration (FHA) Risk Management Initiatives: Revised Seller Concessions...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-28

    ... URBAN DEVELOPMENT 24 CFR Chapter II Federal Housing Administration (FHA) Risk Management Initiatives... Counsel, HUD. ACTION: Correction. SUMMARY: On February 23, 2012 (77 FR 10695), HUD published a request for... methods for submitting public comments. All submissions must refer to the docket number (FR-5572-N-01)...

  17. 76 FR 81930 - Notice of Proposed Administrative Settlement Pursuant to the Comprehensive Environmental Response...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-29

    ... Abbott Mine/Turkey Run Mine Site located in Lake County, California (EPA Docket No. 2011-13). FOR FURTHER... administrative cost recovery settlement concerning the Abbott Mine/Turkey Run Mine Superfund Site (Site) in Lake County, California was executed by the Agency on November 14, 2011. The proposed settlement resolves...

  18. 77 FR 10665 - General Services Administration Acquisition Regulation; Acquisition-Related Thresholds

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-23

    ... 75 FR 53129, August 30, 2010, with an effective date of October 1, 2010. FAR Case 2008-024 implements... thresholds, was published in the Federal Register at 75 FR 53129, August 30, 2010, with an effective date of... ADMINISTRATION 48 CFR Parts 519 and 552 [GSAR Amendment 2012-02; GSAR Case 2011-G502; (Change 54) Docket...

  19. 76 FR 69733 - Proposed CERCLA Administrative Cost Recovery Settlement; Tracy Lead Battery Site, Tracy, MN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-09

    ... AGENCY Proposed CERCLA Administrative Cost Recovery Settlement; Tracy Lead Battery Site, Tracy, MN AGENCY... of past response costs concerning the Tracy Lead Battery Site in Tracy, Minnesota with the following.... Comments should reference the Tracy Lead Battery Site and EPA Docket No. CERCLA-05-2012-0001 and should...

  20. Tetramethylpyrazine inhibits tumor growth of lung cancer through disrupting angiogenesis via BMP/Smad/Id-1 signaling.

    PubMed

    Jia, Youchao; Wang, Zhigang; Zang, Aimin; Jiao, Shunchang; Chen, Sumei; Fu, Yan

    2016-05-01

    The underlying mechanisms of inhibitory effects induced by tetramethylpyrazine (TMP) on angiogenesis and tumor growth of lung cancer were investigated. In vitro cell proliferation, migration, and tube formation of human microvascular endothelial cells (HMEC-1) were evaluated by a 3-(4,5-dimethylthiazol-2-yl)-2,5-dephenyltetrazolium bromide (MTT), wound healing, Transwell, and Matrigel assays. The expression of BMP/Smad/Id-1 signals was detected by RT-PCR and western blotting. In an A549 xenograft tumor model, TMP (40 and 80 mg/kg/day) was intraperitoneally injected into mice. The expressions of CD31, phosphorylated Smad1/5/8, and Id-1 were measured by immunohistochemistry. We demonstrated that TMP inhibited proliferation, migration, and capillary tube formation of HMEC-1 in a dose- and time-dependent manner. Furthermore, treatment of HMEC-1 cells with TMP (0.4 mg/ml) significantly upregulated BMP2 expression and downregulated BMPRIA, BMPRII, phosphorylated Smad1/5/8, and Id-1 expression. In addition, administrations of TMP remarkably inhibited tumor growth of A549 xenograft in nude mice. The CD31, phosphorylated Smad1/5/8, and Id-1 expression were significantly inhibited in TMP-treated xenograft tumors compared with the vehicle. In conclusion, our results indicated that TMP suppressed angiogenesis and tumor growth of lung cancer via blocking the BMP/Smad/Id-1 signaling. PMID:26984046

  1. Id-1 and Id-2 genes and products as therapeutic targets for treatment of breast cancer and other types of carcinoma

    DOEpatents

    Desprez, Pierre-Yves; Campisi, Judith

    2014-09-30

    A method for treatment and amelioration of breast, cervical, ovarian, endometrial, squamous cells, prostate cancer and melanoma in a patient comprising targeting Id-1 or Id-2 gene expression with a delivery vehicle comprising a product which modulates Id-1 or Id-2 expression.

  2. The Antibody Response of Pregnant Cameroonian Women to VAR2CSA ID1-ID2a, a Small Recombinant Protein Containing the CSA-Binding Site

    PubMed Central

    Babakhanyan, Anna; Leke, Rose G. F.; Salanti, Ali; Bobbili, Naveen; Gwanmesia, Philomina; Leke, Robert J. I.; Quakyi, Isabella A.; Chen, John J.; Taylor, Diane Wallace

    2014-01-01

    In pregnant women, Plasmodium falciparum-infected erythrocytes expressing the VAR2CSA antigen bind to chondroitin sulfate A in the placenta causing placental malaria. The binding site of VAR2CSA is present in the ID1-ID2a region. This study sought to determine if pregnant Cameroonian women naturally acquire antibodies to ID1-ID2a and if antibodies to ID1-ID2a correlate with absence of placental malaria at delivery. Antibody levels to full-length VAR2CSA and ID1-ID2a were measured in plasma samples from 745 pregnant Cameroonian women, 144 Cameroonian men, and 66 US subjects. IgM levels and IgG avidity to ID1-ID2a were also determined. As expected, antibodies to ID1-ID2a were absent in US controls. Although pregnant Cameroonian women developed increasing levels of antibodies to full-length VAR2CSA during pregnancy, no increase in either IgM or IgG to ID1-ID2a was observed. Surprisingly, no differences in antibody levels to ID1-ID2a were detected between Cameroonian men and pregnant women. For example, in rural settings only 8–9% of males had antibodies to full-length VAR2CSA, but 90–96% had antibodies to ID1-ID2a. In addition, no significant difference in the avidity of IgG to ID1-ID2a was found between pregnant women and Cameroonian men, and no correlation between antibody levels at delivery and absence of placental malaria was found. Thus, the response to ID1-ID2a was not pregnancy specific, but predominantly against cross-reactivity epitopes, which may have been induced by other PfEMP1 antigens, malarial antigens, or microbes. Currently, ID1-ID2a is a leading vaccine candidate, since it binds to the CSA with the same affinity as the full-length molecule and elicits binding-inhibitory antibodies in animals. Further studies are needed to determine if the presence of naturally acquired cross-reactive antibodies in women living in malaria endemic countries will alter the response to ID1-ID2a following vaccination with ID1-ID2a. PMID:24505415

  3. 416492 ORCID IDs and Counting: Uptake by the Astronomical Community

    NASA Astrophysics Data System (ADS)

    Holmquist, J.; Erdmann, C.; Damon, J.

    2015-04-01

    ORCID — an acronym short for Open Researcher and Contributor ID — is an international, interdisciplinary and community-driven effort to create and maintain a registry of persistent, unique identifiers for researchers and scholars. ORCID IDs are extremely important in the disambiguation of non-unique author names. They can also be embedded in key workflows, such as research profile maintenance, manuscript submissions and grant applications. Using several approaches to reach out to our users, we will report on ORCID ID uptake by the astronomical community.

  4. MicroRNAs and intellectual disability (ID) in Down syndrome, X-linked ID, and Fragile X syndrome

    PubMed Central

    Siew, Wei-Hong; Tan, Kai-Leng; Babaei, Maryam Abbaspour; Cheah, Pike-See; Ling, King-Hwa

    2013-01-01

    Intellectual disability (ID) is one of the many features manifested in various genetic syndromes leading to deficits in cognitive function among affected individuals. ID is a feature affected by polygenes and multiple environmental factors. It leads to a broad spectrum of affected clinical and behavioral characteristics among patients. Until now, the causative mechanism of ID is unknown and the progression of the condition is poorly understood. Advancement in technology and research had identified various genetic abnormalities and defects as the potential cause of ID. However, the link between these abnormalities with ID is remained inconclusive and the roles of many newly discovered genetic components such as non-coding RNAs have not been thoroughly investigated. In this review, we aim to consolidate and assimilate the latest development and findings on a class of small non-coding RNAs known as microRNAs (miRNAs) involvement in ID development and progression with special focus on Down syndrome (DS) and X-linked ID (XLID) [including Fragile X syndrome (FXS)]. PMID:23596395

  5. Study IDs 2 Zika Virus Proteins Linked to Microcephaly

    MedlinePlus

    ... Study IDs 2 Zika Virus Proteins Linked to Microcephaly Discovery might one day lead to a way ... Zika virus linked to the severe birth defect microcephaly. Babies with microcephaly have abnormally small heads and ...

  6. IDS Combined Solution improvements between ITRF2008 and ITRF2013

    NASA Astrophysics Data System (ADS)

    Moreaux, Guilhem; Lemoine, Frank; Willis, Pascal; Capdeville, Hugues; Otten, Michiel; Stepanek, Petr; Kuzin, Sergei; Ferrage, Pascale

    2015-04-01

    In the context of ITRF 2013, the IDS Combination Center delivered to IERS weekly combined SINEX files from early January 1993 to late August 2014. These SINEX files which contain DORIS stations positions/velocities and Earth orientation parameters from DORIS data are based on the latest series of all of the 6 IDS Analysis Centers. The primary objective of this study is to present latest results from the DORIS contribution to ITRF2013. Then, comparisons with IDS contribution to ITRF2008 will address benefits of including both South Atlantic Anomaly corrected data and new DORIS missions (e.g. Jason-1, Jason-2, Cryosat-2) in the IDS combination. These comparisons will also emphasize application of DORIS ground antennas phase laws in the data processing and improved modeling of DORIS ground beacon frequency variations.

  7. Genetics Experts Unite to I.D. Unknown Katrina Victims

    MedlinePlus

    ... Current Issue Past Issues Research News From NIH Genetics Experts Unite to I.D. Unknown Katrina Victims ... in the recent Advances in molecular biology and genetics," says team member Stephen Sherry, Ph.D., of ...

  8. Biometrics and ID Cards — Enablers for Personal Security

    NASA Astrophysics Data System (ADS)

    Reisen, Andreas

    The electronic ID card is a modernization and security project of the German Government. On the one hand, the multifunctional card is intended to boost security and the convenience of e-government and e-business applications. On the other hand, the new biometric ID card should allow citizens to use it as a travel document in the Schengen area and for specific destinations outside the European Union also in the future.

  9. Topological defects in s +is and d +id superconductors

    NASA Astrophysics Data System (ADS)

    Babaev, Egor; Garaud, Julien

    2014-03-01

    Recently arguments were advanced that various compounds can have s +is or d +id superconductivity. I will discuss topological defects which can arise in such superconducting states, their properties and experimental signatures. The allowed defects: vortices, domain walls and Skyrmions all have distinct magnetic features so their observation can be a confirmation of the s +is or d +id nature of superconducting states. Supported by Knut and Alice Wallenberg Foundation, Swedish Research Counlcil and NSF CAREER grant.

  10. BioID Identification of Lamin-Associated Proteins.

    PubMed

    Mehus, Aaron A; Anderson, Ruthellen H; Roux, Kyle J

    2016-01-01

    A- and B-type lamins support the nuclear envelope, contribute to heterochromatin organization, and regulate a myriad of nuclear processes. The mechanisms by which lamins function in different cell types and the mechanisms by which lamin mutations cause over a dozen human diseases (laminopathies) remain unclear. The identification of proteins associated with lamins is likely to provide fundamental insight into these mechanisms. BioID (proximity-dependent biotin identification) is a unique and powerful method for identifying protein-protein and proximity-based interactions in living cells. BioID utilizes a mutant biotin ligase from bacteria that is fused to a protein of interest (bait). When expressed in living cells and stimulated with excess biotin, this BioID-fusion protein promiscuously biotinylates directly interacting and vicinal endogenous proteins. Following biotin-affinity capture, the biotinylated proteins can be identified using mass spectrometry. BioID thus enables screening for physiologically relevant protein associations that occur over time in living cells. BioID is applicable to insoluble proteins such as lamins that are often refractory to study by other methods and can identify weak and/or transient interactions. We discuss the use of BioID to elucidate novel lamin-interacting proteins and its applications in a broad range of biological systems, and provide detailed protocols to guide new applications. PMID:26778550

  11. Anti-ids in allergy: timeliness of a classic concept.

    PubMed

    Wallmann, Julia; Pali-Schöll, Isabella; Jensen-Jarolim, Erika

    2010-06-01

    Anti-idiotypic antibodies (anti-ids) are part of natural immune responses with regulatory capacity. Their effect on an antigen-specific, so-called Ab1 antibody response, is dependent on 1) the original antigen, which they mirror, being Ab2 antibodies, and 2) their isotype. In the case of IgE-mediated allergy, natural anti-ids against allergen-specific IgE represent internal images of allergen molecules. A key biologic feature of allergens is that they can crosslink IgE, expressed by B-lymphocytes or passively bound via high affinity receptors to effector cells, which renders cellular activation. Therefore, the IgE cross linking capability of anti-ids determines whether they dampen or enhance immediate-type hypersensitivity. Correspondingly to classic antiallergen blocking IgG antibodies, anti-ids may also interact with inhibitory FcγRIIb receptors and, thereby, down-regulate T(H)2-type inflammation. Anti-ids and other B-cell epitope mimetics, like mimotopes and DARPins, represent antigen surrogates, which can be used for vaccination. Intriguingly, they may induce antibody responses without activating potentially proinflammatory, antiallergen T-lymphocytes. Taken together, collective evidence suggests that anti-ids, although representing immunologic classics, are a timeless concept in allergology. PMID:21151813

  12. Administrative Synergy

    ERIC Educational Resources Information Center

    Hewitt, Kimberly Kappler; Weckstein, Daniel K.

    2012-01-01

    One of the biggest obstacles to overcome in creating and sustaining an administrative professional learning community (PLC) is time. Administrators are constantly deluged by the tyranny of the urgent. It is a Herculean task to carve out time for PLCs, but it is imperative to do so. In this article, the authors describe how an administrative PLC…

  13. 48 CFR 6101.2 - Filing cases; time limits for filing; notice of docketing; consolidation [Rule 2].

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Filing cases; time limits for filing; notice of docketing; consolidation . 6101.2 Section 6101.2 Federal Acquisition Regulations... Filing cases; time limits for filing; notice of docketing; consolidation . (a) Filing cases. Filing of...

  14. 42 CFR 426.470 - Board's role in docketing and evaluating the acceptability of appeals of ALJ decisions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 3 2010-10-01 2010-10-01 false Board's role in docketing and evaluating the acceptability of appeals of ALJ decisions. 426.470 Section 426.470 Public Health CENTERS FOR MEDICARE & MEDICAID... DETERMINATIONS AND LOCAL COVERAGE DETERMINATIONS Review of an LCD § 426.470 Board's role in docketing...

  15. 20 CFR 501.8 - Clerk of the Office of the Appellate Boards; docket of proceedings; records.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    .... Each docketed appeal will be assigned a number in chronological order based upon the date on which the notice of appeal is received. While the Board generally hears appeals in the order docketed, the Board retains discretion to change the order in which a particular appeal will be considered. The Clerk...

  16. 20 CFR 501.8 - Clerk of the Office of the Appellate Boards; docket of proceedings; records.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    .... Each docketed appeal will be assigned a number in chronological order based upon the date on which the notice of appeal is received. While the Board generally hears appeals in the order docketed, the Board retains discretion to change the order in which a particular appeal will be considered. The Clerk...

  17. 20 CFR 501.8 - Clerk of the Office of the Appellate Boards; docket of proceedings; records.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    .... Each docketed appeal will be assigned a number in chronological order based upon the date on which the notice of appeal is received. While the Board generally hears appeals in the order docketed, the Board retains discretion to change the order in which a particular appeal will be considered. The Clerk...

  18. 49 CFR 837.3 - Published reports, material contained in the public accident investigation dockets, and accident...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    .... For information regarding the types of documents routinely issued by the Board, see 49 CFR part 801... public accident investigation dockets, and accident database data. 837.3 Section 837.3 Transportation... investigation dockets, and accident database data. (a) Demands for material contained in the NTSB's...

  19. 49 CFR 837.3 - Published reports, material contained in the public accident investigation dockets, and accident...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    .... For information regarding the types of documents routinely issued by the Board, see 49 CFR part 801... public accident investigation dockets, and accident database data. 837.3 Section 837.3 Transportation... investigation dockets, and accident database data. (a) Demands for material contained in the NTSB's...

  20. 49 CFR 837.3 - Published reports, material contained in the public accident investigation dockets, and accident...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    .... For information regarding the types of documents routinely issued by the Board, see 49 CFR part 801... public accident investigation dockets, and accident database data. 837.3 Section 837.3 Transportation... investigation dockets, and accident database data. (a) Demands for material contained in the NTSB's...

  1. 49 CFR 837.3 - Published reports, material contained in the public accident investigation dockets, and accident...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    .... For information regarding the types of documents routinely issued by the Board, see 49 CFR part 801... public accident investigation dockets, and accident database data. 837.3 Section 837.3 Transportation... investigation dockets, and accident database data. (a) Demands for material contained in the NTSB's...

  2. 49 CFR 837.3 - Published reports, material contained in the public accident investigation dockets, and accident...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    .... For information regarding the types of documents routinely issued by the Board, see 49 CFR part 801... public accident investigation dockets, and accident database data. 837.3 Section 837.3 Transportation... investigation dockets, and accident database data. (a) Demands for material contained in the NTSB's...

  3. 76 FR 77994 - Brian Hamilton v. El Paso Natural Gas, El Paso Western Pipelines; Notice Announcing Docket Number...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-15

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Brian Hamilton v. El Paso Natural Gas, El Paso Western Pipelines; Notice Announcing Docket Number Change On December 2, 2011, the Commission issued a notice in docket number...

  4. 14 CFR 11.35 - Does FAA include sensitive security information and proprietary information in the Federal Docket...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... RULEMAKING PROCEDURES Rulemaking Procedures General § 11.35 Does FAA include sensitive security information and proprietary information in the Federal Docket Management System (FDMS)? (a) Sensitive security information. You should not submit sensitive security information to the rulemaking docket, unless you...

  5. 14 CFR 11.35 - Does FAA include sensitive security information and proprietary information in the Federal Docket...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... RULEMAKING PROCEDURES Rulemaking Procedures General § 11.35 Does FAA include sensitive security information and proprietary information in the Federal Docket Management System (FDMS)? (a) Sensitive security information. You should not submit sensitive security information to the rulemaking docket, unless you...

  6. 14 CFR 11.35 - Does FAA include sensitive security information and proprietary information in the Federal Docket...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... RULEMAKING PROCEDURES Rulemaking Procedures General § 11.35 Does FAA include sensitive security information and proprietary information in the Federal Docket Management System (FDMS)? (a) Sensitive security information. You should not submit sensitive security information to the rulemaking docket, unless you...

  7. 14 CFR 11.35 - Does FAA include sensitive security information and proprietary information in the Federal Docket...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... RULEMAKING PROCEDURES Rulemaking Procedures General § 11.35 Does FAA include sensitive security information and proprietary information in the Federal Docket Management System (FDMS)? (a) Sensitive security information. You should not submit sensitive security information to the rulemaking docket, unless you...

  8. 14 CFR 11.35 - Does FAA include sensitive security information and proprietary information in the Federal Docket...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... RULEMAKING PROCEDURES Rulemaking Procedures General § 11.35 Does FAA include sensitive security information and proprietary information in the Federal Docket Management System (FDMS)? (a) Sensitive security information. You should not submit sensitive security information to the rulemaking docket, unless you...

  9. The Self- and Other-Deception Questionnaires-Intellectual Disabilities (SDQ-ID and ODQ-ID): component analysis and reliability.

    PubMed

    Jobson, Laura; Stanbury, Alexandra; Langdon, Peter E

    2013-10-01

    The objectives of this research were to: (1) investigate the component structure and psychometric properties of the Self- and Other-Deception Questionnaires-Intellectual Disabilities (SDQ-ID and ODQ-ID), (2) examine the relationship between social desirability and IQ, and (3) compare social desirability scores of those with intellectual disabilities (IDs) and a history of criminal offending to the social desirability scores of participants with IDs and those without IDs and no such history, controlling for general intellectual functioning. Men with mild to borderline IDs detained within medium secure inpatient forensic mental health services (N=40) completed the SDQ-ID and ODQ-ID at Time 1 and then two-weeks later at Time 2. Data for the men with and without IDs and no known criminal offending history were taken from a previous study (N=60). Following exploratory Principal Components Analysis, the number of questionnaire items were reduced, and a two-factor structure was found for the SDQ-ID which was labelled: (1) Positive Self Representation and (2) Denial of Intrusive Thoughts. A two-factor structure was also found for the ODQ-ID and these two factors were labelled: (1) Denial of Negative Social Interaction and (2) Untrustworthiness. Both the SDQ-ID and ODQ-ID had acceptable internal consistency and test-retest reliability. Fifteen percent of the variance in SDQ-ID scores was explained by Full Scale IQ, while 21% of the variance in ODQ-ID scores was explained by Full Scale IQ. Between group comparisons controlling for intelligence did not yield any significant differences. The shortened SDQ-ID and ODQ-ID have promising psychometric properties, and their component structures appear robust. Differences between men with and without IDs on these two measures of social desirability can be accounted for by differences in general intellectual functioning. PMID:23962604

  10. Id2 regulates hyporesponsive invariant natural killer T cells.

    PubMed

    Stradner, Martin H; Cheung, Kitty P; Lasorella, Anna; Goldrath, Ananda W; D'Cruz, Louise M

    2016-08-01

    While the invariant natural killer T (iNKT)-cell response to primary stimulation with the glycolipid, α-galactosylceramide (αGalCer), is robust, the secondary response to this stimulus is muted resulting in a hyporesponsive state characterized by anti-inflammatory interleukin-10 (IL-10) production and high expression of programmed cell death 1 (PD1) and neuropilin 1 (NRP1). The E protein transcription factors and their negative regulators, the Id proteins, have previously been shown to regulate iNKT cell thymic development, subset differentiation and peripheral survival. Here, we provide evidence that the expression of the transcriptional regulator Id2 is downregulated upon stimulation of iNKT cells with their cognate antigen. Moreover, loss of Id2 expression by iNKT cells resulted in a hyporesponsive state, with splenic Id2-deficient iNKT cells expressing low levels of TBET, high levels of PD1 and NRP1 and production of IL-10 upon stimulation. We propose that downregulation of Id2 expression is an essential component of induction of the anti-inflammatory, hyporesponsive state in iNKT cells. PMID:26880074

  11. Id2 regulates hyporesponsive invariant natural killer T cells

    PubMed Central

    Stradner, Martin H; Cheung, Kitty P; Lasorella, Anna; Goldrath, Ananda W; D’Cruz, Louise M

    2016-01-01

    While the invariant natural killer T (iNKT)-cell response to primary stimulation with the glycolipid, α-galactosylceramide (αGalCer), is robust, the secondary response to this stimulus is muted resulting in a hyporesponsive state characterized by anti-inflammatory interleukin-10 (IL-10) production and high expression of programmed cell death 1 (PD1) and neuropilin 1 (NRP1). The E protein transcription factors and their negative regulators, the Id proteins, have previously been shown to regulate iNKT cell thymic development, subset differentiation and peripheral survival. Here, we provide evidence that the expression of the transcriptional regulator Id2 is downregulated upon stimulation of iNKT cells with their cognate antigen. Moreover, loss of Id2 expression by iNKT cells resulted in a hyporesponsive state, with splenic Id2-deficient iNKT cells expressing low levels of TBET, high levels of PD1 and NRP1 and production of IL-10 upon stimulation. We propose that downregulation of Id2 expression is an essential component of induction of the anti-inflammatory, hyporesponsive state in iNKT cells. PMID:26880074

  12. Improving Integration Effectiveness of ID Mapping Based Biological Record Linkage.

    PubMed

    Jamil, Hasan M

    2015-01-01

    Traditionally, biological objects such as genes, proteins, and pathways are represented by a convenient identifier, or ID, which is then used to cross reference, link and describe objects in biological databases. Relationships among the objects are often established using non-trivial and computationally complex ID mapping systems or converters, and are stored in authoritative databases such as UniGene, GeneCards, PIR and BioMart. Despite best efforts, such mappings are largely incomplete and riddled with false negatives. Consequently, data integration using record linkage that relies on these mappings produces poor quality of data, inadvertently leading to erroneous conclusions. In this paper, we discuss this largely ignored dimension of data integration, examine how the ubiquitous use of identifiers in biological databases is a significant barrier to knowledge fusion using distributed computational pipelines, and propose two algorithms for ad hoc and restriction free ID mapping of arbitrary types using online resources. We also propose two declarative statements for ID conversion and data integration based on ID mapping on-the-fly. PMID:26357233

  13. 78 FR 19068 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel GOLDEN BOY II; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-28

    ... BOY II; Invitation for Public Comments AGENCY: Maritime Administration, Department of Transportation... electronic version of this document and all documents entered into this docket is available on the World Wide... the applicant the intended service of the vessel GOLDEN BOY II is: Intended Commercial Use Of...

  14. Modernizing Administration.

    ERIC Educational Resources Information Center

    Lombardi, Vincent L.; Hildebrand, Verna

    1981-01-01

    Suggests assignment of research duties and rotation of teaching and management roles for college administrators, to increase their effectiveness and diminish the negative effects of declining enrollments. (JD)

  15. Id proteins are critical downstream effectors of BMP signaling in human pulmonary arterial smooth muscle cells.

    PubMed

    Yang, Jun; Li, Xiaohui; Li, Ying; Southwood, Mark; Ye, Lingying; Long, Lu; Al-Lamki, Rafia S; Morrell, Nicholas W

    2013-08-15

    Bone morphogenetic protein type II receptor (BMPR-II) mutations are responsible for over 70% of cases of heritable pulmonary arterial hypertension (PAH). Loss of BMP signaling promotes pulmonary vascular remodeling via modulation of pulmonary artery smooth muscle cell (PASMC) proliferation. Id proteins (Id1-4) are major downstream transcriptional targets of BMP signaling. However, the impact of BMPR-II mutation on the expression of the range of Id proteins and the contribution of individual Id proteins to abnormal PASMC function remain unclear. Human PASMCs were used to determine the expression of Id proteins (Id1-4) by real-time PCR and immunoblotting. The BMP responses in control cells were compared with PASMCs harboring BMPR-II mutations and cells in which BMPR-II was knocked down by siRNA transfection. Id3 expression in pulmonary vessels was also investigated in BMPR-II mutant mice and in patients with heritable PAH. BMP4 and BMP6, but not BMP9, induced mRNA expression of Id1, Id2, and Id3. The BMP-stimulated induction of Id1 and Id3 was markedly reduced in BMPR-II mutant PASMCs and in control PASMCs following siRNA silencing of BMPR-II. Pulmonary arteries in BMPR-II mutant mice and patients with heritable PAH demonstrated reduced levels of Id3 compared with control subjects. Lentiviral overexpression of Id3 reduced cell cycle progression and inhibited proliferation of PASMCs. Lipopolysaccharide further reduced Id3 expression in mutant PASMCs. In conclusion, Id proteins, and particularly Id1 and Id3, are critical downstream effectors of BMP signaling in PASMCs. Loss of BMPR-II function reduces the induction of Id genes in PASMCs, Id1, and Id3 regulate the proliferation of PASMCs via cell cycle inhibition, an effect that may be exacerbated by inflammatory stimuli. PMID:23771884

  16. Id proteins are critical downstream effectors of BMP signaling in human pulmonary arterial smooth muscle cells

    PubMed Central

    Yang, Jun; Li, Xiaohui; Li, Ying; Southwood, Mark; Ye, Lingying; Long, Lu; Al-Lamki, Rafia S.

    2013-01-01

    Bone morphogenetic protein type II receptor (BMPR-II) mutations are responsible for over 70% of cases of heritable pulmonary arterial hypertension (PAH). Loss of BMP signaling promotes pulmonary vascular remodeling via modulation of pulmonary artery smooth muscle cell (PASMC) proliferation. Id proteins (Id1–4) are major downstream transcriptional targets of BMP signaling. However, the impact of BMPR-II mutation on the expression of the range of Id proteins and the contribution of individual Id proteins to abnormal PASMC function remain unclear. Human PASMCs were used to determine the expression of Id proteins (Id1–4) by real-time PCR and immunoblotting. The BMP responses in control cells were compared with PASMCs harboring BMPR-II mutations and cells in which BMPR-II was knocked down by siRNA transfection. Id3 expression in pulmonary vessels was also investigated in BMPR-II mutant mice and in patients with heritable PAH. BMP4 and BMP6, but not BMP9, induced mRNA expression of Id1, Id2, and Id3. The BMP-stimulated induction of Id1 and Id3 was markedly reduced in BMPR-II mutant PASMCs and in control PASMCs following siRNA silencing of BMPR-II. Pulmonary arteries in BMPR-II mutant mice and patients with heritable PAH demonstrated reduced levels of Id3 compared with control subjects. Lentiviral overexpression of Id3 reduced cell cycle progression and inhibited proliferation of PASMCs. Lipopolysaccharide further reduced Id3 expression in mutant PASMCs. In conclusion, Id proteins, and particularly Id1 and Id3, are critical downstream effectors of BMP signaling in PASMCs. Loss of BMPR-II function reduces the induction of Id genes in PASMCs, Id1, and Id3 regulate the proliferation of PASMCs via cell cycle inhibition, an effect that may be exacerbated by inflammatory stimuli. PMID:23771884

  17. Clinical and immunological evaluation after BCG-id vaccine in leprosy patients in a 5-year follow-up study

    PubMed Central

    Zenha, Erika Muller Ramalho; Wambier, Carlos Gustavo; Novelino, Ana Lúcia; de Andrade, Thiago Antônio Moretti; Ferreira, Maria Aparecida Nunes; Frade, Marco Andrey Cipriani; Foss, Norma Tiraboschi

    2012-01-01

    Introduction The use of bacillus Calmette–Guérin (BCG) has long been considered a stimulus for immune reactivity in leprosy household contacts. Probably, the combination of multidrug therapy with BCG could facilitate the clearance of leprosy bacilli in the host, reduce relapse rates, and shorten the duration of skin-smear positivity. Methods To investigate the mechanism of action of BCG, a study involving 19 leprosy patients, eleven multibacillary (MB) and eight paucibacillary, was performed to assess the in vitro production of interleukin (IL)-10, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, IL-6, and IL-17 in the supernatant of peripheral blood mononuclear cells, before and 30 days after inoculation with BCG intradermally (BCG-id). Peripheral blood mononuclear cells isolated by Ficoll–Hypaque gradient were cultivated with Concanavalin-A (Con-A), lipopolysccharides (LPS), or BCG. The supernatant was collected for ELISA quantification of cytokines. The immunohistochemistry of IFN-γ, IL-1, IL-10, IL-12, transforming growth factor (TGF)-β, and TNF-α was carried out in biopsies of skin lesions of leprosy patients before and 30 days after inoculation of BCG-id. These patients were followed up for 5 years to assess the therapeutic response to multidrug therapy, the occurrence of leprosy reactions, and the results of bacterial index and anti-PGL-1 serology after the end of treatment. Results The results showed increased production of cytokines after BCG-id administration in MB and paucibacillary leprosy patients. There was statistically higher levels of TNF-α (P = 0.017) in MB patients and of IL-17 (P = 0.008) and IFN-γ (P = 0.037) in paucibacillary patients. Immunohistochemical staining, especially for TNF-α, was more intense in biopsies of MB leprosy patients taken after BCG-id administration, probably for induction of innate human immunity. The clinical evaluation suggests that BCG-id is able to induce a more effective therapeutic response, with

  18. 17 CFR 239.63 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...: For Federal Register citations affecting Form ID, see the List of CFR Sections Affected, which appears... 17 Commodity and Securities Exchanges 2 2011-04-01 2011-04-01 false Form ID, uniform application... Statements § 239.63 Form ID, uniform application for access codes to file on EDGAR. Form ID must be filed...

  19. 17 CFR 269.7 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Federal Register citations affecting Form ID, see the List of CFR Sections Affected, which appears in the... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Form ID, uniform application... Form ID, uniform application for access codes to file on EDGAR. Form ID must be filed by...

  20. 17 CFR 239.63 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...: For Federal Register citations affecting Form ID, see the List of CFR Sections Affected, which appears... 17 Commodity and Securities Exchanges 2 2010-04-01 2010-04-01 false Form ID, uniform application... Statements § 239.63 Form ID, uniform application for access codes to file on EDGAR. Form ID must be filed...

  1. 17 CFR 269.7 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Federal Register citations affecting Form ID, see the List of CFR Sections Affected, which appears in the... 17 Commodity and Securities Exchanges 3 2011-04-01 2011-04-01 false Form ID, uniform application... Form ID, uniform application for access codes to file on EDGAR. Form ID must be filed by...

  2. 17 CFR 249.446 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...: For Federal Register citations affecting Form ID, see the List of CFR Sections Affected, which appears... 17 Commodity and Securities Exchanges 3 2011-04-01 2011-04-01 false Form ID, uniform application... § 249.446 Form ID, uniform application for access codes to file on EDGAR. Form ID must be filed...

  3. 32 CFR 161.7 - ID card life-cycle procedures.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 1 2014-07-01 2014-07-01 false ID card life-cycle procedures. 161.7 Section 161.7 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE SECURITY IDENTIFICATION (ID) CARDS FOR MEMBERS OF THE UNIFORMED SERVICES, THEIR DEPENDENTS, AND OTHER ELIGIBLE INDIVIDUALS DoD Identification (ID) Cards: ID Card...

  4. 76 FR 80980 - Notice of Acceptance for Docketing of the Application, Notice of Opportunity for Hearing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-27

    ... accordance with the NRC E-Filing rule (72 FR 49139, August 28, 2007). The E-Filing process requires... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Notice of Acceptance for Docketing of the Application, Notice of Opportunity for...

  5. 33 CFR 148.207 - How and where may I view docketed documents?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false How and where may I view docketed documents? 148.207 Section 148.207 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) DEEPWATER PORTS DEEPWATER PORTS: GENERAL Processing Applications General § 148.207 How and where may I view...

  6. 15 CFR 400.32 - Procedures for docketing applications and commencement of case review.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Procedures for docketing applications and commencement of case review. 400.32 Section 400.32 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF...

  7. 15 CFR 400.31 - General application provisions and pre-docketing review.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false General application provisions and pre-docketing review. 400.31 Section 400.31 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE...

  8. 15 CFR 400.31 - General application provisions and pre-docketing review.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false General application provisions and pre-docketing review. 400.31 Section 400.31 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE...

  9. 15 CFR 400.32 - Procedures for docketing applications and commencement of case review.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Procedures for docketing applications and commencement of case review. 400.32 Section 400.32 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF...

  10. 78 FR 16668 - Twenty-Fifth Update of the Federal Agency Hazardous Waste Compliance Docket

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-18

    ... 12, 1988 (53 FR 4280). Since then, updates to the Docket have been published on November 16, 1988 (54 FR 46364); December 15, 1989 (54 FR 51472); August 22, 1990 (55 FR 34492); September 27, 1991 (56 FR 49328); December 12, 1991 (56 FR 64898); July 17, 1992 (57 FR 31758); February 5, 1993 (58 FR...

  11. 75 FR 2163 - Constellation Energy; Notice of Docketing of Special Nuclear Material License SNM-2505 Amendment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-14

    ... COMMISSION Constellation Energy; Notice of Docketing of Special Nuclear Material License SNM-2505 Amendment... June 15, 2009, from Constellation Energy (Constellation) to amend its Special Nuclear Material License... will remain the same for this action. An NRC review, documented in a letter to Constellation dated...

  12. 75 FR 16100 - Antimicrobial Pesticide Registration Review Dockets Opened for Review and Comment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-31

    ...EPA has established registration review dockets for the pesticides listed in the table in Unit III.A. With this document, EPA is opening the public comment period for these registration reviews. Registration review is EPA's periodic review of pesticide registrations to ensure that each pesticide continues to satisfy the statutory standard for registration, that is, the pesticide can perform......

  13. 75 FR 16117 - Registration Review; Pesticide Dockets Opened for Review and Comment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-31

    ...EPA has established registration review dockets for the pesticides listed in the table in Unit III.A. With this document, EPA is opening the public comment period for these registration reviews. Registration review is EPA's periodic review of pesticide registrations to ensure that each pesticide continues to satisfy the statutory standard for registration, that is, the pesticide can perform......

  14. 75 FR 38521 - Registration Review; Biopesticide Dockets Opened for Review and Comment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-02

    ... registration review dockets for the pesticides listed in the table in Unit III.A. of this notice. With this... EPA's periodic review of pesticide registrations to ensure that each pesticide continues to satisfy... specific pesticide of interest provided in the table in Unit III.A. of this notice, by one of the...

  15. 75 FR 16114 - Registration Review; Biopesticides Dockets Opened for Review and Comment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-31

    ...EPA has established registration review dockets for the pesticides listed in the table in Unit III.A. With this document, EPA is opening the public comment period for these registration reviews. Registration review is EPA's periodic review of pesticide registrations to ensure that each pesticide continues to satisfy the statutory standard for registration, that is, the pesticide can perform......

  16. 76 FR 31606 - Cryolite Registration Review Docket; Extension of Comment Period

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-01

    ... of March 30, 2011 (76 FR 17646) (FRL-8868-9). In that document, the Agency announced the availability... AGENCY Cryolite Registration Review Docket; Extension of Comment Period AGENCY: Environmental Protection... comment, including cryolite. This document extends the comment period for the cryolite registration...

  17. 76 FR 79173 - Registration Review; Pesticide Dockets Opened for Review and Comment, and Notice of Availability...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-21

    ... of Final Work Plans for Certain Pesticides AGENCY: Environmental Protection Agency (EPA). ACTION... availability of amended final work plans for the registration review of the pesticides cryolite, prodiamine... amended final work plans for the registration review of the pesticides cryolite (docket EPA-HQ-OPP-...

  18. 78 FR 56947 - Prairie Island; Independent Spent Fuel Storage Installation; Notice of Docketing of Amendment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-16

    ... accordance with the NRC E-Filing rule (72 FR 49139; August 28, 2007). The E-Filing process requires... that the filing requirements of the NRC's E-Filing Rule (72 FR 49139; August 28, 2007) apply to appeals... COMMISSION Prairie Island; Independent Spent Fuel Storage Installation; Notice of Docketing of...

  19. 40 CFR 1612.3 - Published reports and material contained in the public incident investigation dockets.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false Published reports and material... Published reports and material contained in the public incident investigation dockets. (a) Demands for published investigation reports should be directed to the Office of Congressional and Public Affairs,...

  20. 77 FR 66609 - Twenty-Fifth Update of the Federal Agency Hazardous Waste Compliance Docket

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-06

    ... (53 FR 4280). Since then, updates to the Docket have been published on November 16, 1988 (54 FR 46364); December 15, 1989 (54 FR 51472); August 22, 1990 (55 FR 34492); September 27, 1991 (56 FR 49328); December 12, 1991 (56 FR 64898); July 17, 1992 (57 FR 31758); February 5, 1993 (58 FR 7298); November 10,...

  1. Administrative Support.

    ERIC Educational Resources Information Center

    Doran, Dorothy; And Others

    This guide is intended to assist business education teachers in administrative support courses. The materials presented are based on the Arizona validated occupational competencies and tasks for the occupations of receptionist, secretary, and administrative assistant. Word processing skills have been infused into each of the three sections. The…

  2. Administrative Ecology

    ERIC Educational Resources Information Center

    McGarity, Augustus C., III; Maulding, Wanda

    2007-01-01

    This article discusses how all four facets of administrative ecology help dispel the claims about the "impossibility" of the superintendency. These are personal ecology, professional ecology, organizational ecology, and community ecology. Using today's superintendency as an administrative platform, current literature describes a preponderance of…

  3. A Simple Quantum Integro-Differential Solver (SQuIDS)

    NASA Astrophysics Data System (ADS)

    Argüelles Delgado, Carlos A.; Salvado, Jordi; Weaver, Christopher N.

    2015-11-01

    Simple Quantum Integro-Differential Solver (SQuIDS) is a C++ code designed to solve semi-analytically the evolution of a set of density matrices and scalar functions. This is done efficiently by expressing all operators in an SU(N) basis. SQuIDS provides a base class from which users can derive new classes to include new non-trivial terms from the right hand sides of density matrix equations. The code was designed in the context of solving neutrino oscillation problems, but can be applied to any problem that involves solving the quantum evolution of a collection of particles with Hilbert space of dimension up to six.

  4. Installation, commissioning and performance of IDs installed at ALBA

    NASA Astrophysics Data System (ADS)

    Campmany, J.; Marcos, J.; Massana, V.; Becheri, F.; Gigante, J. V.; Colldelram, C.; Ribó, Ll

    2013-03-01

    The new synchrotron light source ALBA is currently starting regular operation. Up to 6 beamlines are using light produced by Insertion Devices. There are up to four types of IDs: 2 Apple-II undulators (EU62 and EU71) operating at low energies, one conventional wiggler (MPW80) operating in the range of 2 - 20 keV, two in-vacuum undulators (IVU21) operating in the range 5 - 30 keV and a superconducting wiggler (SCW30) operating in the range of (up to) 40 keV. The main IDs characteristics, their influence on the beam dynamics and a first characterization of their light will be presented.

  5. Identification of Staphylococcus species and subspecies with the MicroScan Pos ID and Rapid Pos ID panel systems.

    PubMed

    Kloos, W E; George, C G

    1991-04-01

    The accuracies of the MicroScan Pos ID and Rapid Pos ID panel systems (Baxter Diagnostic Inc., MicroScan Division, West Sacramento, Calif.) were compared with each other and with the accuracies of conventional methods for the identification of 25 Staphylococcus species and 4 subspecies. Conventional methods included those used in the original descriptions of species and subspecies and DNA-DNA hybridization. The Pos ID panel uses a battery of 18 tests, and the Rapid Pos ID panel uses a battery of 42 tests for the identification of Staphylococcus species. The Pos ID panel has modified conventional and chromogenic tests that can be read after 15 to 48 h of incubation; the Rapid Pos ID panel has tests that use fluorogenic substrates or fluorometric indicators, and test results can be read after 2 h of incubation in the autoSCAN-W/A. Results indicated that both MicroScan systems had a high degree of congruence (greater than or equal to 90%) with conventional methods for the species S. capitis, S. aureus, S. auricularis, S. saprophyticus, S. cohnii, S. arlettae, S. carnosus, S. lentus, and S. sciuri and, in particular, the subspecies S. capitis subsp. capitis and S. cohnii subsp. cohnii. The Rapid Pos ID panel system also had greater than or equal to 90% congruence with conventional methods for S. epidermidis, S. caprae, S. warneri subsp. 2, S. xylosus, S. kloosii, and S. caseolyticus. For both MicroScan systems, congruence with conventional methods was 80 to 90% for S. haemolyticus subsp. 1, S. equorum, S. intermedius, and S. hyicus; and in addition, with the Rapid Pos ID panel system congruence was 80 to 89% for S. capitis subsp. ureolyticus, S. warneri subsp. 1, S. hominis, S. cohnii subsp. urealyticum, and S. simulans. The MicroScan systems identified a lower percentage (50 to 75%) of strains of S. lugdunensis, S. gallinarum, S. schleiferi, and S. chromogenes, although the addition of specific tests to the systems might increase the accuracy of identification

  6. Identification of Staphylococcus species and subspecies with the MicroScan Pos ID and Rapid Pos ID panel systems.

    PubMed Central

    Kloos, W E; George, C G

    1991-01-01

    The accuracies of the MicroScan Pos ID and Rapid Pos ID panel systems (Baxter Diagnostic Inc., MicroScan Division, West Sacramento, Calif.) were compared with each other and with the accuracies of conventional methods for the identification of 25 Staphylococcus species and 4 subspecies. Conventional methods included those used in the original descriptions of species and subspecies and DNA-DNA hybridization. The Pos ID panel uses a battery of 18 tests, and the Rapid Pos ID panel uses a battery of 42 tests for the identification of Staphylococcus species. The Pos ID panel has modified conventional and chromogenic tests that can be read after 15 to 48 h of incubation; the Rapid Pos ID panel has tests that use fluorogenic substrates or fluorometric indicators, and test results can be read after 2 h of incubation in the autoSCAN-W/A. Results indicated that both MicroScan systems had a high degree of congruence (greater than or equal to 90%) with conventional methods for the species S. capitis, S. aureus, S. auricularis, S. saprophyticus, S. cohnii, S. arlettae, S. carnosus, S. lentus, and S. sciuri and, in particular, the subspecies S. capitis subsp. capitis and S. cohnii subsp. cohnii. The Rapid Pos ID panel system also had greater than or equal to 90% congruence with conventional methods for S. epidermidis, S. caprae, S. warneri subsp. 2, S. xylosus, S. kloosii, and S. caseolyticus. For both MicroScan systems, congruence with conventional methods was 80 to 90% for S. haemolyticus subsp. 1, S. equorum, S. intermedius, and S. hyicus; and in addition, with the Rapid Pos ID panel system congruence was 80 to 89% for S. capitis subsp. ureolyticus, S. warneri subsp. 1, S. hominis, S. cohnii subsp. urealyticum, and S. simulans. The MicroScan systems identified a lower percentage (50 to 75%) of strains of S. lugdunensis, S. gallinarum, S. schleiferi, and S. chromogenes, although the addition of specific tests to the systems might increase the accuracy of identification

  7. Phosphorylation Regulates Id2 Degradation and Mediates the Proliferation of Neural Precursor Cells

    PubMed Central

    Sullivan, Jaclyn M.; Havrda, Matthew C.; Kettenbach, Arminja N.; Paolella, Brenton R.; Zhang, Zhonghua; Gerber, Scott A.; Israel, Mark A.

    2016-01-01

    Inhibitor of DNA binding proteins (Id1-Id4) function to inhibit differentiation and promote proliferation of many different cell types. Among the Id family members, Id2 has been most extensively studied in the central nervous system (CNS). Id2 contributes to cultured neural precursor cell (NPC) proliferation as well as to the proliferation of CNS tumors such as glioblastoma that are likely to arise from NPC-like cells. We identified three phosphorylation sites near the N-terminus of Id2 in NPCs. To interrogate the importance of Id2 phosphorylation, Id2−/− NPCs were modified to express wild type (WT) Id2 or an Id2 mutant protein that could not be phosphorylated at the identified sites. We observed that NPCs expressing this mutant lacking phosphorylation near the N-terminus had higher steady-state levels of Id2 when compared to NPCs expressing WT Id2. This elevated level was the result of a longer half-life and reduced proteasome-mediated degradation. Moreover, NPCs expressing constitutively de-phosphorylated Id2 proliferated more rapidly than NPCs expressing WT Id2, a finding consistent with the well-characterized function of Id2 in driving proliferation. Observing that phosphorylation of Id2 modulates the degradation of this important cell-cycle regulator, we sought to identify a phosphatase that would stabilize Id2 enhancing its activity in NPCs and extended our analysis to include human glioblastoma-derived stem cells (GSCs). We found that expression of the phosphatase PP2A altered Id2 levels. Our findings suggest that inhibition of PP2A may be a novel strategy to regulate the proliferation of normal NPCs and malignant GSCs by decreasing Id2 levels. PMID:26756672

  8. Phosphorylation Regulates Id2 Degradation and Mediates the Proliferation of Neural Precursor Cells.

    PubMed

    Sullivan, Jaclyn M; Havrda, Matthew C; Kettenbach, Arminja N; Paolella, Brenton R; Zhang, Zhonghua; Gerber, Scott A; Israel, Mark A

    2016-05-01

    Inhibitor of DNA binding proteins (Id1-Id4) function to inhibit differentiation and promote proliferation of many different cell types. Among the Id family members, Id2 has been most extensively studied in the central nervous system (CNS). Id2 contributes to cultured neural precursor cell (NPC) proliferation as well as to the proliferation of CNS tumors such as glioblastoma that are likely to arise from NPC-like cells. We identified three phosphorylation sites near the N-terminus of Id2 in NPCs. To interrogate the importance of Id2 phosphorylation, Id2(-/-) NPCs were modified to express wild type (WT) Id2 or an Id2 mutant protein that could not be phosphorylated at the identified sites. We observed that NPCs expressing this mutant lacking phosphorylation near the N-terminus had higher steady-state levels of Id2 when compared to NPCs expressing WT Id2. This elevated level was the result of a longer half-life and reduced proteasome-mediated degradation. Moreover, NPCs expressing constitutively de-phosphorylated Id2 proliferated more rapidly than NPCs expressing WT Id2, a finding consistent with the well-characterized function of Id2 in driving proliferation. Observing that phosphorylation of Id2 modulates the degradation of this important cell-cycle regulator, we sought to identify a phosphatase that would stabilize Id2 enhancing its activity in NPCs and extended our analysis to include human glioblastoma-derived stem cells (GSCs). We found that expression of the phosphatase PP2A altered Id2 levels. Our findings suggest that inhibition of PP2A may be a novel strategy to regulate the proliferation of normal NPCs and malignant GSCs by decreasing Id2 levels. Stem Cells 2016;34:1321-1331. PMID:26756672

  9. An Application of Decision Tree Based on ID3

    NASA Astrophysics Data System (ADS)

    Xiaohu, Wang; Lele, Wang; Nianfeng, Li

    This article deals with the application of classical decision tree ID3 of the data mining in a certain site data. It constitutes a decision tree based on information gain and thus produces some useful purchasing behavior rules. It also proves that the decision tree has a wide applicable future in the sale field on site.

  10. 77 FR 55688 - Amendment of Class E Airspace; Boise, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-11

    ... FR 6026). Interested parties were invited to participate in this rulemaking effort by submitting... E airspace designated as an extension at Boise Air Terminal, Boise, ID (77 FR 38552). Interested... Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation...

  11. Enabling OpenID Authentication for VO-integrated Portals

    NASA Astrophysics Data System (ADS)

    Plante, R.; Yekkirala, V.; Baker, W.

    2012-09-01

    To support interoperating services that share proprietary data and other user-specific information, the VAO Project provides login services for browser-based portals built on the open standard, OpenID. To help portal developers take advantage of this service, we have developed a downloadable toolkit for integrating OpenID single sign-on support into any portal. This toolkit provides APIs in a few languages commonly used on the server-side as well as a command-line version for use in any language. In addition to describing how to use this toolkit, we also discuss the general VAO framework for single sign-on. While a portal may, if it wishes, support any OpenID provider, the VAO service provides a few extra features to support VO interoperability. This includes a portal's ability to retrieve (with the user's permission) an X.509 certificate representing the authenticated user so that the portal can access other restricted services on the user's behalf. Other standard features of OpenID allow portals to request other information about the user; this feature will be used in the future for sharing information about a user's group membership to enable sharing within a group of collaborating scientists.

  12. Blueprints for Complex Learning: The 4C/ID-Model.

    ERIC Educational Resources Information Center

    van Merrienboer, Jeroen J. G.; Clark, Richard E.; de Croock, Marcel B. M.

    2002-01-01

    Describes the four-component instructional design system (4C/ID-model) developed for the design of training programs for complex skills. Discusses the structure of training blueprints for complex learning and associated instructional methods, focusing on learning tasks, supportive information, just-in-time information, and part-task practice.…

  13. 75 FR 12974 - Establishment of Class E Airspace; Hailey, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-18

    ... Register a notice of proposed rulemaking to establish additional controlled airspace at Hailey, ID (74 FR... Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of... continues to read as follows: Authority: 49 U.S.C. 106(g), 40103, 40113, 40120; E. O. 10854, 24 FR 9565,...

  14. Retention Revisited: R=E, Id+E & In, Iv.

    ERIC Educational Resources Information Center

    Seidman, Alan

    1996-01-01

    Recent research on college student attrition is examined for trends, and it is suggested that the common perspective on retention and attrition is too narrow; it should be viewed from three perspectives: within-course retention; program retention; and institutional retention rate. Recommended for retention (R) is early (E) identification (Id) plus…

  15. 75 FR 61610 - Modification of Class E Airspace; Arco, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-06

    ...This action will amend Class E airspace at Arco, ID. Decommissioning of the Arco-Butte County Non-Directional Beacon (NDB) at Arco-Butte County Airport has made this action necessary for the safety and management of Instrument Flight Rules (IFR) operations at the airport. This action also would adjust the geographic coordinates of the...

  16. idRHa+ProMod - Rail Hardening Control System

    NASA Astrophysics Data System (ADS)

    Ferro, L.

    2016-03-01

    idRHa+ProMod is the process control system developed by Primetals Technologies to foresee the thermo-mechanical evolution and micro-structural composition of rail steels subjected to slack quenching into idRHa+ Rail Hardening equipments in a simulation environment. This tool can be used both off-line or in-line, giving the user the chance to test and study the best cooling strategies or letting the automatic control system free to adjust the proper cooling recipe. Optimization criteria have been tailored in order to determine the best cooling conditions according to the metallurgical requirements imposed by the main rail standards and also taking into account the elastoplastic bending phenomena occurring during all stages of the head hardening process. The computational core of idRHa+ProMod is a thermal finite element procedure coupled with special algorithms developed to work out the main thermo-physical properties of steel, to predict the non-isothermal austenite decomposition into all the relevant phases and subsequently to evaluate the amount of latent heat of transformation released, the compound thermal expansion coefficient and the amount of plastic deformation in the material. Air mist and air blades boundary conditions have been carefully investigated by means of pilot plant tests aimed to study the jet impingement on rail surfaces and the cooling efficiency at all working conditions. Heat transfer coefficients have been further checked and adjusted directly on field during commissioning. idRHa+ is a trademark of Primetals Technologies Italy Srl

  17. Comparison of Perinatal Risk Factors Associated with Autism Spectrum Disorder (ASD), Intellectual Disability (ID), and Co-occurring ASD and ID.

    PubMed

    Schieve, Laura A; Clayton, Heather B; Durkin, Maureen S; Wingate, Martha S; Drews-Botsch, Carolyn

    2015-08-01

    While studies report associations between perinatal outcomes and both autism spectrum disorder (ASD) and intellectual disability (ID), there has been little study of ASD with versus without co-occurring ID. We compared perinatal risk factors among 7547 children in the 2006-2010 Autism and Developmental Disability Monitoring Network classified as having ASD + ID, ASD only, and ID only. Children in all three groups had higher rates of preterm birth (PTB), low birth weight, small-for-gestational-age, and low Apgar score than expected based on the US birth cohort adjusted for key socio-demographic factors. Associations with most factors, especially PTB, were stronger for children with ID only than children with ASD + ID or ASD only. Associations were similar for children with ASD + ID and ASD only. PMID:25739693

  18. Inhibitor of Differentiation 4 (ID4) Acts as an Inhibitor of ID-1, -2 and -3 and Promotes basic Helix Loop Helix (bHLH) E47 DNA Binding and Transcriptional Activity

    PubMed Central

    Sharma, Pankaj; Chinaranagari, Swathi; Chaudhary, Jaideep

    2015-01-01

    The four known ID proteins (ID1-4, Inhibitor of Differentiation) share a homologous helix loop helix (HLH) domain and act as dominant negative regulators of basic-HLH transcription factors. ID proteins also interact with many non-bHLH proteins in complex networks. The expression of ID proteins is increasingly observed in many cancers. Whereas ID-1, ID-2 and ID-3, are generally considered as tumor promoters, ID4 on the contrary has emerged as a tumor suppressor. In this study we demonstrate that ID4 heterodimerizes with ID-1, -2 and -3 and promote bHLH DNA binding, essentially acting as an inhibitor of inhibitors of differentiation proteins. Interaction of ID4 was observed with ID1, ID2 and ID3 that was dependent on intact HLH domain of ID4. Interaction with bHLH protein E47 required almost 3 fold higher concentration of ID4 as compared to ID1. Furthermore, inhibition of E47 DNA binding by ID1 was restored by ID4 in an EMSA binding assay. ID4 and ID1 were also colocalized in prostate cancer cell line LNCaP. The alpha helix forming alanine stretch N-terminal, unique to HLH ID4 domain was required for optimum interaction. Ectopic expression of ID4 in DU145 prostate cancer line promoted E47 dependent expression of CDKNI p21. Thus counteracting the biological activities of ID-1, -2 and -3 by forming inactive heterodimers appears to be a novel mechanism of action of ID4. These results could have far reaching consequences in developing strategies to target ID proteins for cancer therapy and understanding biologically relevant ID- interactions. PMID:25778840

  19. Inhibitor of differentiation 4 (ID4) acts as an inhibitor of ID-1, -2 and -3 and promotes basic helix loop helix (bHLH) E47 DNA binding and transcriptional activity.

    PubMed

    Sharma, Pankaj; Chinaranagari, Swathi; Chaudhary, Jaideep

    2015-05-01

    The four known ID proteins (ID1-4, Inhibitor of Differentiation) share a homologous helix loop helix (HLH) domain and act as dominant negative regulators of basic-HLH transcription factors. ID proteins also interact with many non-bHLH proteins in complex networks. The expression of ID proteins is increasingly observed in many cancers. Whereas ID-1, ID-2 and ID-3, are generally considered as tumor promoters, ID4 on the contrary has emerged as a tumor suppressor. In this study we demonstrate that ID4 heterodimerizes with ID-1, -2 and -3 and promote bHLH DNA binding, essentially acting as an inhibitor of inhibitors of differentiation proteins. Interaction of ID4 was observed with ID1, ID2 and ID3 that was dependent on intact HLH domain of ID4. Interaction with bHLH protein E47 required almost 3 fold higher concentration of ID4 as compared to ID1. Furthermore, inhibition of E47 DNA binding by ID1 was restored by ID4 in an EMSA binding assay. ID4 and ID1 were also colocalized in prostate cancer cell line LNCaP. The alpha helix forming alanine stretch N-terminal, unique to HLH ID4 domain was required for optimum interaction. Ectopic expression of ID4 in DU145 prostate cancer line promoted E47 dependent expression of CDKNI p21. Thus counteracting the biological activities of ID-1, -2 and -3 by forming inactive heterodimers appears to be a novel mechanism of action of ID4. These results could have far reaching consequences in developing strategies to target ID proteins for cancer therapy and understanding biologically relevant ID-interactions. PMID:25778840

  20. Id2 intrinsically regulates lymphoid and erythroid development via interaction with different target proteins

    PubMed Central

    Ji, Ming; Li, Huajie; Suh, Hyung Chan; Klarmann, Kimberly D.; Yokota, Yoshifumi

    2008-01-01

    Inhibitors of DNA binding (Id) family members are key regulators of cellular differentiation and proliferation. These activities are related to the ability of Id proteins to antagonize E proteins and other transcription factors. As negative regulators of E proteins, Id proteins have been implicated in lymphocyte development. Overexpression of Id1, Id2, or Id3 has similar effects on lymphocyte development. However, which Id protein plays a physiologic role during lymphocyte development is not clear. By analyzing Id2 knock-out mice and retroviral transduced hematopoietic progenitors, we demonstrated that Id2 is an intrinsic negative regulator of B-cell development. Hematopoietic progenitor cells overexpressing Id2 did not reconstitute B-cell development in vivo, which resembled the phenotype of E2A null mice. The B-cell population in bone marrow was significantly expanded in Id2 knock-out mice compared with their wild-type littermates. Knock-down of Id2 by shRNA in hematopoietic progenitor cells promoted B-cell differentiation and induced the expression of B-cell lineage–specific genes. These data identified Id2 as a physiologically relevant regulator of E2A during B lymphopoiesis. Furthermore, we identified a novel Id2 function in erythroid development. Overexpression of Id2 enhanced erythroid development, and decreased level of Id2 impaired normal erythroid development. Id2 regulation of erythroid development is mediated via interacting with transcription factor PU.1 and modulating PU.1 and GATA-1 activities. We conclude that Id2 regulates lymphoid and erythroid development via interaction with different target proteins. PMID:18523151

  1. Evaluation of red blood cell and platelet antigen genotyping platforms (ID CORE XT/ID HPA XT) in routine clinical practice

    PubMed Central

    Finning, Kirstin; Bhandari, Radhika; Sellers, Fiona; Revelli, Nicoletta; Villa, Maria Antonietta; Muñiz-Díaz, Eduardo; Nogués, Núria

    2016-01-01

    Background High-throughput genotyping platforms enable simultaneous analysis of multiple polymorphisms for blood group typing. BLOODchip® ID is a genotyping platform based on Luminex® xMAP technology for simultaneous determination of 37 red blood cell (RBC) antigens (ID CORE XT) and 18 human platelet antigens (HPA) (ID HPA XT) using the BIDS XT software. Materials and methods In this international multicentre study, the performance of ID CORE XT and ID HPA XT, using the centres’ current genotyping methods as the reference for comparison, and the usability and practicality of these systems, were evaluated under working laboratory conditions. DNA was extracted from whole blood in EDTA with Qiagen methodologies. Ninety-six previously phenotyped/genotyped samples were processed per assay: 87 testing samples plus five positive controls and four negative controls. Results Results were available for 519 samples: 258 with ID CORE XT and 261 with ID HPA XT. There were three “no calls” that were either caused by human error or resolved after repeating the test. Agreement between the tests and reference methods was 99.94% for ID CORE XT (9,540/9,546 antigens determined) and 100% for ID HPA XT (all 4,698 alleles determined). There were six discrepancies in antigen results in five RBC samples, four of which (in VS, N, S and Doa) could not be investigated due to lack of sufficient sample to perform additional tests and two of which (in S and C) were resolved in favour of ID CORE XT (100% accuracy). The total hands-on time was 28–41 minutes for a batch of 16 samples. Compared with the reference platforms, ID CORE XT and ID HPA XT were considered simpler to use and had shorter processing times. Discussion ID CORE XT and ID HPA XT genotyping platforms for RBC and platelet systems were accurate and user-friendly in working laboratory settings. PMID:26674823

  2. WSS25, a sulfated polysaccharide, inhibits RANKL-induced mouse osteoclast formation by blocking SMAD/ID1 signaling

    PubMed Central

    Chen, Cheng; Qin, Yi; Fang, Jian-ping; Ni, Xin-yan; Yao, Jian; Wang, Hai-ying; Ding, Kan

    2015-01-01

    Aim: WSS25 is a sulfated polysaccharide extracted from the rhizome of Gastrodia elata BI, which has been found to bind to bone morphogenetic protein 2 (BMP-2) in hepatocellular cancer cells. Since BMP-2 may regulate both osteoclasts and osteoblasts, here we investigated the effects of WSS25 on osteoclastogenesis in vitro and bone loss in ovariectomized mice. Methods: RAW264.7 cells or mouse bone marrow macrophages (BMMs) were treated with RANKL to induce osteoclastogenesis, which was assessed using TRAP staining, actin ring formation and pit formation assays, as well as bone resorption assay. Cell viability was detected with MTT assay. The mRNA levels of osteoclastogenesis-related genetic markers (TRAP, NFATc1, MMP-9 and cathepsin K) were detected using RT-PCR, while the protein levels of p-Smad1/5/8 and Id1 were measure with Western blotting. WSS25 was administered to ovariectomized mice (100 mg·kg−1·d−1, po) for 3 months. After the mice were euthanized, total bone mineral density and cortical bone density were measured. Results: In RAW264.7 cells and BMMs, WSS25 (2.5, 5, 10 μg/mL) did not affect the cell viability, but dose-dependently inhibited RANKL-induced osteoclastogenesis. Furthermore, WSS25 potently suppressed RANKL-induced expression of TRAP, NFATc1, MMP-9 and cathepsin K in RAW264.7 cells. Treatment of RAW264.7 cells with RANKL increased BMP-2 expression, Smad1/5/8 phosphorylation and Id1 expression, which triggered osteoclast differentiation, whereas co-treatment with WSS25 or the endogenous BMP-2 antagonist noggin suppressed the BMP-2/Smad/Id1 signaling pathway. In RAW264.7 cells, knockdown of Id1 attenuated RANKL-induced osteoclast differentiation, which was partially rescued by Id1 overexpression. In conformity to the in vitro experiments, chronic administration of WSS25 significantly reduced the bone loss in ovariectomized mice. Conclusion: WSS25 inhibits RANKL-induced osteoclast formation in RAW264.7 cells and BMMs by blocking the BMP-2/Smad/Id

  3. Effect of the silybin-phosphatidylcholine complex (IdB 1016) on the development of mammary tumors in HER-2/neu transgenic mice.

    PubMed

    Provinciali, Mauro; Papalini, Francesca; Orlando, Fiorenza; Pierpaoli, Sara; Donnini, Alessia; Morazzoni, Paolo; Riva, Antonella; Smorlesi, Arianna

    2007-03-01

    Silybin, a main component of the milk thistle of Silybum marianum, has been reported to possess anticancer activity. We investigated the effects of IdB 1016, a complex of silybin with phosphatidylcholine, on the development of mammary tumors appearing spontaneously in HER-2/neu transgenic mice. The mechanisms involved in the antitumor effect of IdB 1016 were evaluated by studying the apoptosis, senescent-like growth arrest, intratumoral leukocyte infiltrate, and the expression of HER-2/neu and p53 in tumoral mammary glands from transgenic mice and in human breast SKBR3 tumor cells. The administration of IdB 1016 delayed the development of spontaneous mammary tumors, reduced the number and size of mammary tumor masses, and diminished lung metastasization in HER-2/neu transgenic mice. In tumoral mammary glands from IdB 1016-treated mice, a down-regulation of HER-2/neu gene expression was associated with an increased senescent-like growth arrest of tumor cells, and an increased infiltrate of neutrophils, CD4, and CD8 T cells. Both senescent-like growth arrest and apoptosis were significantly increased and were associated with a reduced p185(HER-2/neu) protein and an increased p53 mRNA in SKBR3 in vitro treated with IdB 1016 in comparison with control cells. The results show the antitumor effect of IdB 1016 in the development of spontaneous mammary tumors in HER-2/neu transgenic mice. The effect of IdB 1016 might be related to the down-regulation of HER-2/neu expression and the induction of senescent-like growth arrest and apoptosis through a p53-mediated pathway in tumor cells. PMID:17332330

  4. Id Proteins Regulate Capillary Repair and Perivascular Cell Proliferation following Ischemia-Reperfusion Injury

    PubMed Central

    Lee, David; Shenoy, Shantheri; Nigatu, Yezina; Plotkin, Matt

    2014-01-01

    Acute kidney injury (AKI) results in microvascular damage that if not normally repaired, may lead to fibrosis. The Id1 and 3 proteins have a critical role in promoting angiogenesis during development, tumor growth and wound repair by functioning as dominant negative regulators of bHLH transcription factors. The goal of this study was to determine if Id proteins regulate microvascular repair and remodeling and if increased Id1 expression results in decreased capillary loss following AKI. The effect of changes in Id expression in vivo was examined using Id1−/−, Id3RFP/+ (Id1/Id3 KO) and Tek (Tie2)-rtTA, TRE-lacz/TRE Id1 (TRE Id1) mice with doxycycline inducible endothelial Id1 and β-galactosidase expression. Id1 and 3 were co-localized in endothelial cells in normal adult kidneys and protein levels were increased at day 3 following ischemia-reperfusion injury (IRI) and contralateral nephrectomy. Id1/Id3 KO mice had decreased baseline capillary density and pericyte coverage and increased tubular damage following IRI but decreased interstitial cell proliferation and fibrosis compared with WT littermates. No compensatory increase in kidney size occurred in KO mice resulting in increased creatinine compared with WT and TRE Id1 mice. TRE Id1 mice had no capillary rarefaction within 1 week following IRI in comparison with WT littermates. TRE Id1 mice had increased proliferation of PDGFRβ positive interstitial cells and medullary collagen deposition and developed capillary rarefaction and albuminuria at later time points. These differences were associated with increased Angiopoietin 1 (Ang1) and decreased Ang2 expression in TRE Id1 mice. Examination of gene expression in microvascular cells isolated from WT, Id1/Id3 KO and TRE Id1 mice showed increased Ang1 and αSMA in Id1 overexpressing cells and decreased pericyte markers in cells from KO mice. These results suggest that increased Id levels following AKI result in microvascular remodeling associated with increased

  5. TLR4 and TLR7/8 Adjuvant Combinations Generate Different Vaccine Antigen-Specific Immune Outcomes in Minipigs when Administered via the ID or IN Routes.

    PubMed

    McKay, Paul F; King, Deborah F L; Mann, Jamie F S; Barinaga, Guillermo; Carter, Darrick; Shattock, Robin J

    2016-01-01

    The induction of high levels of systemic and mucosal humoral immunity is a key goal for many prophylactic vaccines. However, adjuvant strategies developed in mice have often performed poorly in the clinic. Due to their closer similarity to humans, minipigs may provide a more accurate picture of adjuvant performance. Based on their complementary signalling pathways, we assessed humoral immune responses to model antigens after co-administration with the toll-like receptor 4 (TLR4) stimulator glucopyranosyl lipid adjuvant (GLA-AF) or the TLR7/8 agonist resiquimod (R848) (alone and in combination) via the intradermal (ID), intranasal (IN) or combined routes in the Gottingen minipig animal model. Surprisingly, we discovered that while GLA-AF additively enhanced the adjuvant effect of R848 when injected ID, it abrogated the adjuvant activity of R848 after IN inoculation. We then performed a route comparison study using a CN54 gp140 HIV Envelope model antigen adjuvanted with R848 + GLA-AF (ID) or R848 alone (IN). Animals receiving priming inoculations via one route were then boosted by the alternate route. Although differences were observed in the priming phase (IN or ID), responses converged upon boosting by the alternative route with no observable impact resultant from the order of administration (ID/IN vs IN/ID). Specific IgG responses were measured at a distal mucosal site (vaginal), although there was no evidence of mucosal linkage as these closely reflected serum antibody levels. These data indicate that the complex in vivo cross-talk between innate pathways are likely tissue specific and cannot be predicted by simple in vitro models. PMID:26862758

  6. TLR4 and TLR7/8 Adjuvant Combinations Generate Different Vaccine Antigen-Specific Immune Outcomes in Minipigs when Administered via the ID or IN Routes

    PubMed Central

    McKay, Paul F.; King, Deborah F. L.; Mann, Jamie F. S.; Barinaga, Guillermo; Carter, Darrick; Shattock, Robin J.

    2016-01-01

    The induction of high levels of systemic and mucosal humoral immunity is a key goal for many prophylactic vaccines. However, adjuvant strategies developed in mice have often performed poorly in the clinic. Due to their closer similarity to humans, minipigs may provide a more accurate picture of adjuvant performance. Based on their complementary signalling pathways, we assessed humoral immune responses to model antigens after co-administration with the toll-like receptor 4 (TLR4) stimulator glucopyranosyl lipid adjuvant (GLA-AF) or the TLR7/8 agonist resiquimod (R848) (alone and in combination) via the intradermal (ID), intranasal (IN) or combined routes in the Gottingen minipig animal model. Surprisingly, we discovered that while GLA-AF additively enhanced the adjuvant effect of R848 when injected ID, it abrogated the adjuvant activity of R848 after IN inoculation. We then performed a route comparison study using a CN54 gp140 HIV Envelope model antigen adjuvanted with R848 + GLA-AF (ID) or R848 alone (IN). Animals receiving priming inoculations via one route were then boosted by the alternate route. Although differences were observed in the priming phase (IN or ID), responses converged upon boosting by the alternative route with no observable impact resultant from the order of administration (ID/IN vs IN/ID). Specific IgG responses were measured at a distal mucosal site (vaginal), although there was no evidence of mucosal linkage as these closely reflected serum antibody levels. These data indicate that the complex in vivo cross-talk between innate pathways are likely tissue specific and cannot be predicted by simple in vitro models. PMID:26862758

  7. Administrative IT

    ERIC Educational Resources Information Center

    Grayson, Katherine, Ed.

    2006-01-01

    When it comes to Administrative IT solutions and processes, best practices range across the spectrum. Enterprise resource planning (ERP), student information systems (SIS), and tech support are prominent and continuing areas of focus. But widespread change can also be accomplished via the implementation of campuswide document imaging and sharing,…

  8. Engineering Administration.

    ERIC Educational Resources Information Center

    Naval Personnel Program Support Activity, Washington, DC.

    This book is intended to acquaint naval engineering officers with their duties in the engineering department. Standard shipboard organizations are analyzed in connection with personnel assignments, division operations, and watch systems. Detailed descriptions are included for the administration of directives, ship's bills, damage control, training…

  9. ADMINISTRATIVE CLIMATE.

    ERIC Educational Resources Information Center

    BRUCE, ROBERT L.; CARTER, G.L., JR.

    IN THE COOPERATIVE EXTENSION SERVICE, STYLES OF LEADERSHIP PROFOUNDLY AFFECT THE QUALITY OF THE SERVICE RENDERED. ACCORDINGLY, MAJOR INFLUENCES ON ADMINISTRATIVE CLIMATE AND EMPLOYEE PRODUCTIVITY ARE EXAMINED IN ESSAYS ON (1) SOURCES OF JOB SATISFACTION AND DISSATISFACTION, (2) MOTIVATIONAL THEORIES BASED ON JOB-RELATED SATISFACTIONS AND NEEDS,…

  10. Database Administrator

    ERIC Educational Resources Information Center

    Moore, Pam

    2010-01-01

    The Internet and electronic commerce (e-commerce) generate lots of data. Data must be stored, organized, and managed. Database administrators, or DBAs, work with database software to find ways to do this. They identify user needs, set up computer databases, and test systems. They ensure that systems perform as they should and add people to the…

  11. Entitlement Management: Ready to Enter the IdM Mainstream

    NASA Astrophysics Data System (ADS)

    Gebel, Gerry; Wang, Alice

    Externalizing authorization processing from business applications has been a goal for architects and developers for many years. Recent compliance demands for more granular access control and policy transparency have increased the urgency to adopt an architectural model where authorization and entitlement management are consumed as a service rather than embedded within business applications. Major software vendors, such as Microsoft, Oracle, and SAP, are making significant steps to separate IdM functionality, including entitlement management from application logic. The market has also responded with a plethora of products that provide entitlement management and authorization functionality for commercial or custom-built application environments. Finally, the Extensible Access Control Markup Language (XACML) has emerged as the consensus authorization standard being supported by a growing number of IT and application vendors. Collectively, these forces form the basis of a nascent IdM market segment referred to as entitlement management.

  12. Status and evolution of the ESRF beamline ID19

    SciTech Connect

    Weitkamp, Timm; Tafforeau, Paul; Boller, Elodie; Cloetens, Peter; Valade, Jean-Paul; Bernard, Pascal; Baruchel, Jose; Peyrin, Francoise; Helfen, Lukas

    2010-04-06

    The ESRF synchrotron beamline ID19, dedicated to full-field parallel-beam imaging techniques such as phase-contrast and absorption microtomography and X-ray topography, is one of the most versatile instruments of its kind. This paper presents key characteristics of ID19 in its present form, names examples for research and development performed on the beamline, and outlines the plans for an upgrade on the beamline in coming years, to adapt to the growing needs of the user community. The technical goals envisioned include an increase in available beam size and maximum photon energy, and a substantial increase in flux density for applications using beams of small and intermediate size.

  13. 78 FR 52190 - United States Department of Energy, Bonneville Power Administration: Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket Nos. EF13-7-000, EF13-8-000, EF13-9-000, EF13-10-000, EF13-11- 000, EF13-12-000, EF13-13-000, EF13-14-000] United States Department of Energy, Bonneville Power Administration: Notice of Filing Take notice that...

  14. 75 FR 10811 - Notice of Application for Recordable Disclaimer of Interest in Lands, Gem County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-09

    ... County, ID AGENCY: Bureau of Land Management, Interior. ACTION: Notice. SUMMARY: An application has been... State Director, Bureau of Land Management, Idaho State Office, 1387 S. Vinnell Way, Boise, ID 83709....

  15. 78 FR 8596 - Hartford Financial Services Group, Inc., Commercial/Actuarial/ Information Delivery Services (IDS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-06

    .../ Information Delivery Services (IDS)/Corporate & Financial Reporting Group, Hartford, CT; Notice of Negative... was published in the Federal Register on January 4, 2013 (78 FR 773). The Hartford-IDS Group...

  16. Essential functions for ID proteins at multiple checkpoints in natural killer T cell development

    PubMed Central

    Verykokakis, Mihalis; Krishnamoorthy, Veena; Iavarone, Antonio; Lasorella, Anna; Sigvardsson, Mikael; Kee, Barbara L.

    2013-01-01

    Invariant natural killer T (iNKT) cells display characteristics of both adaptive and innate lymphoid cells (ILCs). Like other ILCs, iNKT cells constitutively express ID proteins, which antagonize the E protein transcription factors that are essential for adaptive lymphocyte development. However, unlike ILCs, ID2 is not essential for thymic iNKT cell development. Here we demonstrated that ID2 and ID3 redundantly promoted iNKT cell lineage specification involving the induction of the signature transcription factor PLZF and that ID3 was critical for development of TBET-dependent NKT1 cells. In contrast, both ID2 and ID3 limited iNKT cell numbers by enforcing the post-selection checkpoint in conventional thymocytes. Therefore, iNKT cells show both adaptive and innate-like requirements for ID proteins at distinct checkpoints during iNKT cell development. PMID:24244015

  17. 3. Photocopy of a photograph (from the General Services Administration, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Photocopy of a photograph (from the General Services Administration, Auburn, Washington) Photographer unknown, November 1962 IMMIGRATION RESIDENCE PRIOR TO ALTERATION OF THE PORCH STAIRS AND INSTALLATION OF SCREENS ON THE FRONT PORCH, VIEW TO THE SOUTHWEST - U.S. Border Inspection Station, Immigation Officers Residence, West side of State Route 95, Eastport, Boundary County, ID

  18. Suppression of Chondrogenesis by Id Helix-Loop-Helix Proteins in Murine Embryonic Orofacial Tissue

    PubMed Central

    Mukhopadhyay, Partha; Rezzoug, Francine; Webb, Cynthia L.; Pisano, M. Michele; Greene, Robert M.

    2009-01-01

    Inhibitors of differentiation (Id) proteins are helix-loop-helix (HLH) transcription factors lacking a DNA binding domain. Id proteins modulate cell proliferation, apoptosis, and differentiation in embryonic/fetal tissue. Perturbation of any of these processes in cells of the developing orofacial region results in orofacial anomalies. Chondrogenesis, a process integral to normal orofacial ontogenesis, is known to be modulated, in part, by Id proteins. In the present study, the mRNA and protein expression patterns of Id1, Id2, Id3 and Id4 were examined in developing murine orofacial tissue in vivo, as well as in murine embryonic maxillary mesenchymal cells in vitro. The functional role of Ids during chondrogenesis was also explored in vitro. Results reveal that cells derived from developing murine orofacial tissue: (1) express Id1, Id2, Id3 and Id4 mRNAs and proteins on each of gestational days 12-14, (2) express all four Id proteins in a developmentally regulated manner, (3) undergo chondrogenesis and express genes encoding various chondrogenic marker proteins (e.g. Runx2, Type X collagen, Sox9) when cultured under micromass conditions, and (4) can have their chondrogenic potential regulated via alteration of Id protein function through overexpression of a basic HLH factor. In summary, results from the current report reveal for the first time, the expression of all four Id proteins in cells derived from developing murine orofacial tissue, and demonstrate a functional role for the Ids in regulating the ability of these cells to undergo chondrogenesis. PMID:19349107

  19. ETR BASEMENT, TRA642, INTERIOR. BASEMENT. CUBICLE INTERIOR (SEE PHOTOS ID33G101 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    ETR BASEMENT, TRA-642, INTERIOR. BASEMENT. CUBICLE INTERIOR (SEE PHOTOS ID-33-G-101 AND ID-33-G-102) WITH TANK AND SODIUM-RELATED APPARATUS. CAMERA STANDS BEFORE ROLL-UP DOOR SHOWN IN PHOTO ID-33-G-101. INL NEGATIVE NO. HD24-3-3. Mike Crane, Photographer, 11/2000 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  20. Study of multilayer-reflected beam profiles and their coherence properties using beamlines ID19 (ESRF) and 32-ID (APS)

    SciTech Connect

    Rack, A.; Assoufid, L.; Dietsch, R.; Weitkamp, T.; Trabelsi, S. Bauer; Rack, T.; Siewert, F.; Kraemer, M.; Holz, Th.; Zanette, I.; Le, W.-K.; Cloetens, P.; Ziegler, E.

    2012-05-17

    The use of multilayer mirrors is an interesting alternative for reflective X-ray monochromatization with respect to reflection from crystal optics. The increased photon flux density due to the multilayers' larger bandwidth is of crucial importance for, e.g, full-field X-ray imaging applications. Drawbacks are the introduced modifications of the reflected beam profile as well as a certain loss of coherence, summarized as wavefront degradation. Our recent work has shown that the modification of the beam profile can vary with, e.g., the material composition of the coating applied. In order to verify our findings, a beamline round-robin has been initiated, comparing the wavefront profiles after reflection by selected multilayers at beamlines 32-ID (Advanced Photon Source) and ID19 (European Synchrotron Radiation Facility) with our initial results acquired at BM05 (ESRF) [1].

  1. Iron deficiency (ID) at both birth and 9 months predicts right frontal EEG asymmetry in infancy.

    PubMed

    Armony-Sivan, Rinat; Zhu, Bingquan; Clark, Katy M; Richards, Blair; Ji, Chai; Kaciroti, Niko; Shao, Jie; Lozoff, Betsy

    2016-05-01

    This study considered effects of timing and duration of iron deficiency (ID) on frontal EEG asymmetry in infancy. In healthy term Chinese infants, EEG was recorded at 9 months in three experimental conditions: baseline, peek-a-boo, and stranger approach. Eighty infants provided data for all conditions. Prenatal ID was defined as low cord ferritin or high ZPP/H. Postnatal ID was defined as ≥ two abnormal iron measures at 9 months. Study groups were pre- and postnatal ID, prenatal ID only, postnatal ID only, and not ID. GLM repeated measure analysis showed a main effect for iron group. The pre- and postnatal ID group had negative asymmetry scores, reflecting right frontal EEG asymmetry (mean ± SE: -.18 ± .07) versus prenatal ID only (.00 ± .04), postnatal ID only (.03 ± .04), and not ID (.02 ± .04). Thus, ID at both birth and 9 months was associated with right frontal EEG asymmetry, a neural correlate of behavioral withdrawal and negative emotions. © 2015 Wiley Periodicals, Inc. Dev Psychobiol 58: 462-470, 2016. PMID:26668100

  2. 6 CFR 37.23 - Reissued REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 6 Domestic Security 1 2011-01-01 2011-01-01 false Reissued REAL ID driver's licenses and... SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.23 Reissued REAL ID driver's licenses and identification cards. (a)...

  3. 77 FR 37696 - Agency Information Collection Activities: Importer ID Input Record

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-22

    ... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Importer ID Input... other Federal agencies to comment on an information collection requirement concerning the Importer ID... concerning the following information collection: Title: Importer ID Input Record. OMB Number: 1651-0064....

  4. 17 CFR 274.402 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... its Password. Editorial Note: For Federal Register citations affecting Form ID, see the List of CFR... 17 Commodity and Securities Exchanges 3 2011-04-01 2011-04-01 false Form ID, uniform application... Forms for Electronic Filing § 274.402 Form ID, uniform application for access codes to file on...

  5. 78 FR 67383 - Agency Information Collection Activities; Importer ID Input Record

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-12

    ... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities; Importer ID Input... other Federal agencies to comment on an information collection requirement concerning the Importer ID... concerning the following information collection: Title: Importer ID Input Record. OMB Number: 1651-0064....

  6. 6 CFR 37.25 - Renewal of REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 6 Domestic Security 1 2011-01-01 2011-01-01 false Renewal of REAL ID driver's licenses and... SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.25 Renewal of REAL ID driver's licenses and identification cards. (a)...

  7. 75 FR 57053 - Camas National Wildlife Refuge, Jefferson County, ID; Comprehensive Conservation Plan and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-17

    ... Fish and Wildlife Service Camas National Wildlife Refuge, Jefferson County, ID; Comprehensive... prepare a comprehensive conservation plan (CCP) for Camas National Wildlife Refuge (refuge) in Hamer, ID... Refuge, 2150 East 2350 North, Hamer, ID 83425. In-Person Drop-off: You may drop off comments...

  8. A Role for Id2 in Regulating Photic Entrainment of the Mammalian Circadian System

    PubMed Central

    Duffield, Giles E.; Watson, Nathan P.; Mantani, Akio; Peirson, Stuart N.; Robles-Murguia, Maricela; Loros, Jennifer J.; Israel, Mark A.; Dunlap, Jay C.

    2009-01-01

    Summary Inhibitor of DNA binding genes (Id1–Id4) encode helix-loop-helix (HLH) transcriptional repressors associated with development and tumorigenesis [1, 2], but little is known concerning the function(s) of these genes in normal adult animals. Id2 was identified in DNA microarray screens for rhythmically expressed genes [3–5], and further analysis revealed a circadian pattern of expression of all four Id genes in multiple tissues including the suprachiasmatic nucleus. To explore an in vivo function, we generated and characterized deletion mutations of Id2 and of Id4. Id2−/− mice exhibit abnormally rapid entrainment and an increase in the magnitude of the phase shift of the pacemaker. A significant proportion of mice also exhibit disrupted rhythms when maintained under constant darkness. Conversely, Id4−/− mice did not exhibit a noticeable circadian phenotype. In vitro studies using an mPer1 and an AVP promoter reporter revealed the potential for ID1, ID2, and ID3 proteins to interact with the canonical basic HLH clock proteins BMAL1 and CLOCK. These data suggest that the Id genes may be important for entrainment and operation of the mammalian circadian system, potentially acting through BMAL1 and CLOCK targets. PMID:19217292

  9. Picture This: How to Establish an Effective School ID Card Program

    ERIC Educational Resources Information Center

    Finkelstein, David

    2013-01-01

    Most school districts do not have an ID card policy that everyone knows and follows, yet. many school districts are implementing ID card programs to address concerns about safety, efficiency, and convenience. A well-thought-out ID card program leads to greater security and smoother operations throughout the school and should thus be a priority.…

  10. Long March-ID performance and possibility of providing launch service

    NASA Astrophysics Data System (ADS)

    Li, Yiming

    A review is presented of the Chinese Long March-ID (LM-ID), a three-stage vehicle to launch small payloads of several hundred kilograms into LEO. The principal advantages of the LM-ID are significant payload capability, proven reliability and reasonable costs. Attention is given to the design philosophy, configuration details, performance capability and launch service modes.

  11. 6 CFR 37.25 - Renewal of REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 6 Domestic Security 1 2012-01-01 2012-01-01 false Renewal of REAL ID driver's licenses and... SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.25 Renewal of REAL ID driver's licenses and identification cards. (a)...

  12. 6 CFR 37.25 - Renewal of REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 6 Domestic Security 1 2013-01-01 2013-01-01 false Renewal of REAL ID driver's licenses and... SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.25 Renewal of REAL ID driver's licenses and identification cards. (a)...

  13. 6 CFR 37.25 - Renewal of REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 6 Domestic Security 1 2014-01-01 2014-01-01 false Renewal of REAL ID driver's licenses and... SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.25 Renewal of REAL ID driver's licenses and identification cards. (a)...

  14. 6 CFR 37.23 - Reissued REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 6 Domestic Security 1 2013-01-01 2013-01-01 false Reissued REAL ID driver's licenses and... SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.23 Reissued REAL ID driver's licenses and identification cards. (a)...

  15. 6 CFR 37.23 - Reissued REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 6 Domestic Security 1 2014-01-01 2014-01-01 false Reissued REAL ID driver's licenses and... SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.23 Reissued REAL ID driver's licenses and identification cards. (a)...

  16. 6 CFR 37.23 - Reissued REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 6 Domestic Security 1 2012-01-01 2012-01-01 false Reissued REAL ID driver's licenses and... SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.23 Reissued REAL ID driver's licenses and identification cards. (a)...

  17. Id3 and Id2 act as a dual safety mechanism in regulating the development and population size of innate-like γδ T cells

    PubMed Central

    Dai, Meifang; Zhuang, Yuan

    2014-01-01

    The innate-like T cells expressing Vγ1.1 and Vδ6.3 represent a unique T cell lineage sharing features with both the γδ T and the invariant NKT cells. The population size of Vγ1.1+Vδ6.3+ T cells is tightly controlled and usually contributes to a very small proportion of thymic output, but the underlying mechanism remains enigmatic. Deletion of Id3, an inhibitor of E-protein transcription factors, can induce an expansion of the Vγ1.1+Vδ6.3+ T cell population. This phenotype is much stronger on the C57Bl/6 background than on the 129/sv background. Using quantitative trait linkage analysis, we identified Id2, a homologue of Id3, to be the major modifier of Id3 in limiting Vγ1.1+Vδ6.3+ T cell expansion. The Vγ1.1+Vδ6.3+ phenotype is attributed to an intrinsic weakness of Id2 transcription from Id2 C57Bl/6 allele, leading to an overall reduced dosage of Id proteins. However, complete removal of both Id2 and Id3 genes in developing T cells suppressed the expansion of Vγ1.1+Vδ6.3+ T cells due to decreased proliferation and increased cell death. We showed that conditional knockout of Id2 alone is sufficient to promote a moderate expansion of γδ T cells. These regulatory effects of Id2 and Id3 on Vγ1.1+Vδ6.3+ T cells are mediated by titration of E protein activity, since removing one or more copies of E protein genes can restore Vγ1.1+Vδ6.3+ T cell expansion in Id2 and Id3 double conditional knockout mice. Our data indicated that Id2 and Id3 collaboratively control survival and expansion of the γδ lineage through modulating a proper threshold of E-proteins. PMID:24379125

  18. Id3 and Id2 act as a dual safety mechanism in regulating the development and population size of innate-like γδ T cells.

    PubMed

    Zhang, Baojun; Lin, Yen-Yu; Dai, Meifang; Zhuang, Yuan

    2014-02-01

    The innate-like T cells expressing Vγ1.1 and Vδ6.3 represent a unique T cell lineage sharing features with both the γδ T and the invariant NKT cells. The population size of Vγ1.1(+)Vδ6.3(+) T cells is tightly controlled and usually contributes to a very small proportion of thymic output, but the underlying mechanism remains enigmatic. Deletion of Id3, an inhibitor of E protein transcription factors, can induce an expansion of the Vγ1.1(+)Vδ6.3(+) T cell population. This phenotype is much stronger on the C57BL/6 background than on the 129/sv background. Using quantitative trait linkage analysis, we identified Id2, a homolog of Id3, to be the major modifier of Id3 in limiting Vγ1.1(+)Vδ6.3(+) T cell expansion. The Vγ1.1(+)Vδ6.3(+) phenotype is attributed to an intrinsic weakness of Id2 transcription from Id2 C57BL/6 allele, leading to an overall reduced dosage of Id proteins. However, complete removal of both Id2 and Id3 genes in developing T cells suppressed the expansion of Vγ1.1(+)Vδ6.3(+) T cells because of decreased proliferation and increased cell death. We showed that conditional knockout of Id2 alone is sufficient to promote a moderate expansion of γδ T cells. These regulatory effects of Id2 and Id3 on Vγ1.1(+)Vδ6.3(+) T cells are mediated by titration of E protein activity, because removing one or more copies of E protein genes can restore Vγ1.1(+)Vδ6.3(+) T cell expansion in Id2 and Id3 double conditional knockout mice. Our data indicated that Id2 and Id3 collaboratively control survival and expansion of the γδ lineage through modulating a proper threshold of E proteins. PMID:24379125

  19. Id2 specifically alters regulation of the cell cycle by tumor suppressor proteins.

    PubMed Central

    Lasorella, A; Iavarone, A; Israel, M A

    1996-01-01

    Cells which are highly proliferative typically lack expression of differentiated, lineage-specific characteristics. Id2, a member of the helix-loop-helix (HLH) protein family known to inhibit cell differentiation, binds to the retinoblastoma protein (pRb) and abolishes its growth-suppressing activity. We found that Id2 but not Id1 or Id3 was able to bind in vitro not only pRb but also the related proteins p107 and p130. Also, an association between Id2 and p107 or p130 was observed in vivo in transiently transfected Saos-2 cells. In agreement with these results, expression of Id1 or Id3 did not affect the block of cell cycle progression mediated by pRb. Conversely, expression of Id2 specifically reversed the cell cycle arrest induced by each of the three members of the pRb family. Furthermore, the growth-suppressive activities of cyclin-dependent kinase inhibitors p16 and p21 were efficiently antagonized by high levels of Id2 but not by Id1 Id3. Consistent with the role of p16 as a selective inhibitor of pRb and pRb-related protein kinase activity, p16-imposed cell cycle arrest was completely abolished by Id2. Only a partial reversal of p21-induced growth suppression was observed, which correlated with the presence of a functional pRb. We also documented decreased levels of cyclin D1 protein and mRNA and the loss of cyclin D1-cdk4 complexes in cells constitutively expressing Id2. These data provide evidence for important Id2-mediated alterations in cell cycle components normally involved in the regulatory events of cell cycle progression, and they highlight a specific role for Id2 as an antagonist of multiple tumor suppressor proteins. PMID:8649364

  20. Iron nanoparticles significantly affect the in vitro and in vivo expression of Id genes.

    PubMed

    Zou, Jinglu; Wang, Xin; Zhang, Ling; Wang, Jinke

    2015-03-16

    In recent DNA microarray studies, we found that the transcription of the Id3 gene was significantly down-regulated in five cell lines (RAW264.7, Hepa1-6, THP-1, HepG2, and HL7702) treated with two doses (50 and 100 μg/mL) of a DMSA-coated magnetite nanoparticle. Given the regulatory roles of Id genes in the cell cycle, growth, and differentiation, we wanted to do more investigations on the effect of the nanoparticle upon the Id genes. This study detected the expression of Id genes in six cell lines (the above cell lines plus HeLa) treated with the nanoparticle at the same doses using quantitative PCR. The results revealed that the expression of Id genes was significantly affected by the nanoparticle in these cell lines. Under each treatment, the Id3 gene was significantly (p < 0.01) down-regulated in all cell lines, the Id1 gene was significantly down-regulated in all cell lines except the RAW264.7 cells, and the Id2 gene was significantly down-regulated in the HepG2, HL7702, and HeLa cells. Because the Id1, Id2, and Id3 genes were significantly down-regulated in three liver-derived cell lines (Hepa1-6, HepG2, and HL7702) in both microarray and PCR detections, this study then detected the expression of Id genes in the liver tissues of mice that were intravenously injected with the nanoparticle at two doses (2 and 5 mg/kg body weight). The results revealed that the expression of Id1, Id2, and Id3 genes was also significantly down-regulated in the liver tissues under each treatment. Another Id gene, Id4, was also significantly regulated in some cells or liver tissues treated with the nanoparticle. These results reveal that the nanoparticle exerts a significant effect on the in vitro and in vivo expression of Id genes. This study thus provides new insights into the Id-related nanotoxicity of the nanoparticle and the close relationship between the regulation of Id genes and iron. PMID:25522732

  1. Impaired Thermogenesis and a Molecular Signature for Brown Adipose Tissue in Id2 Null Mice.

    PubMed

    Zhou, Peng; Robles-Murguia, Maricela; Mathew, Deepa; Duffield, Giles E

    2016-01-01

    Inhibitor of DNA binding 2 (ID2) is a helix-loop-helix transcriptional repressor rhythmically expressed in many adult tissues. Our previous studies have demonstrated that Id2 null mice have sex-specific elevated glucose uptake in brown adipose tissue (BAT). Here we further explored the role of Id2 in the regulation of core body temperature over the circadian cycle and the impact of Id2 deficiency on genes involved in insulin signaling and adipogenesis in BAT. We discovered a reduced core body temperature in Id2-/- mice. Moreover, in Id2-/- BAT, 30 genes including Irs1, PPARs, and PGC-1s were identified as differentially expressed in a sex-specific pattern. These data provide valuable insights into the impact of Id2 deficiency on energy homeostasis of mice in a sex-specific manner. PMID:27144179

  2. Impaired Thermogenesis and a Molecular Signature for Brown Adipose Tissue in Id2 Null Mice

    PubMed Central

    Zhou, Peng; Robles-Murguia, Maricela; Mathew, Deepa; Duffield, Giles E.

    2016-01-01

    Inhibitor of DNA binding 2 (ID2) is a helix-loop-helix transcriptional repressor rhythmically expressed in many adult tissues. Our previous studies have demonstrated that Id2 null mice have sex-specific elevated glucose uptake in brown adipose tissue (BAT). Here we further explored the role of Id2 in the regulation of core body temperature over the circadian cycle and the impact of Id2 deficiency on genes involved in insulin signaling and adipogenesis in BAT. We discovered a reduced core body temperature in Id2−/− mice. Moreover, in Id2−/− BAT, 30 genes including Irs1, PPARs, and PGC-1s were identified as differentially expressed in a sex-specific pattern. These data provide valuable insights into the impact of Id2 deficiency on energy homeostasis of mice in a sex-specific manner. PMID:27144179

  3. CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing

    PubMed Central

    Dehairs, Jonas; Talebi, Ali; Cherifi, Yacine; Swinnen, Johannes V.

    2016-01-01

    The advent of next generation gene editing technologies has revolutionized the fields of genome engineering in allowing the generation of gene knockout models and functional gene analysis. However, the screening of resultant clones remains challenging due to the simultaneous presence of different indels. Here, we present CRISP-ID, a web application which uses a unique algorithm for genotyping up to three alleles from a single Sanger sequencing trace, providing a robust and readily accessible platform to directly identify indels and significantly speed up the characterization of clones. PMID:27363488

  4. CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing.

    PubMed

    Dehairs, Jonas; Talebi, Ali; Cherifi, Yacine; Swinnen, Johannes V

    2016-01-01

    The advent of next generation gene editing technologies has revolutionized the fields of genome engineering in allowing the generation of gene knockout models and functional gene analysis. However, the screening of resultant clones remains challenging due to the simultaneous presence of different indels. Here, we present CRISP-ID, a web application which uses a unique algorithm for genotyping up to three alleles from a single Sanger sequencing trace, providing a robust and readily accessible platform to directly identify indels and significantly speed up the characterization of clones. PMID:27363488

  5. Quadruplex Integrated DNA (QuID) Nanosensors for Monitoring Dopamine

    PubMed Central

    Morales, Jennifer M.; Skipwith, Christopher G.; Clark, Heather A.

    2015-01-01

    Dopamine is widely innervated throughout the brain and critical for many cognitive and motor functions. Imbalances or loss in dopamine transmission underlie various psychiatric disorders and degenerative diseases. Research involving cellular studies and disease states would benefit from a tool for measuring dopamine transmission. Here we show a Quadruplex Integrated DNA (QuID) nanosensor platform for selective and dynamic detection of dopamine. This nanosensor exploits DNA technology and enzyme recognition systems to optically image dopamine levels. The DNA quadruplex architecture is designed to be compatible in physically constrained environments (110 nm) with high flexibility, homogeneity, and a lower detection limit of 110 µM. PMID:26287196

  6. d+id' Chiral Superconductivity in Bilayer Silicene

    NASA Astrophysics Data System (ADS)

    Liu, Feng; Liu, Cheng-Cheng; Wu, Kehui; Yang, Fan; Yao, Yugui

    2013-08-01

    We investigate the structure and physical properties of the undoped bilayer silicene through first-principles calculations and find the system is intrinsically metallic with sizable pocket Fermi surfaces. When realistic electron-electron interaction turns on, the system is identified as a chiral d+id' topological superconductor mediated by the strong spin fluctuation on the border of the antiferromagnetic spin density wave order. Moreover, the tunable Fermi pocket area via strain makes it possible to adjust the spin density wave critical interaction strength near the real one and enables a high superconducting critical temperature.

  7. Microbe-ID: an open source toolbox for microbial genotyping and species identification

    PubMed Central

    Tabima, Javier F.; Everhart, Sydney E.; Larsen, Meredith M.; Weisberg, Alexandra J.; Kamvar, Zhian N.; Tancos, Matthew A.; Smart, Christine D.; Chang, Jeff H.

    2016-01-01

    Development of tools to identify species, genotypes, or novel strains of invasive organisms is critical for monitoring emergence and implementing rapid response measures. Molecular markers, although critical to identifying species or genotypes, require bioinformatic tools for analysis. However, user-friendly analytical tools for fast identification are not readily available. To address this need, we created a web-based set of applications called Microbe-ID that allow for customizing a toolbox for rapid species identification and strain genotyping using any genetic markers of choice. Two components of Microbe-ID, named Sequence-ID and Genotype-ID, implement species and genotype identification, respectively. Sequence-ID allows identification of species by using BLAST to query sequences for any locus of interest against a custom reference sequence database. Genotype-ID allows placement of an unknown multilocus marker in either a minimum spanning network or dendrogram with bootstrap support from a user-created reference database. Microbe-ID can be used for identification of any organism based on nucleotide sequences or any molecular marker type and several examples are provided. We created a public website for demonstration purposes called Microbe-ID (microbe-id.org) and provided a working implementation for the genus Phytophthora (phytophthora-id.org). In Phytophthora-ID, the Sequence-ID application allows identification based on ITS or cox spacer sequences. Genotype-ID groups individuals into clonal lineages based on simple sequence repeat (SSR) markers for the two invasive plant pathogen species P. infestans and P. ramorum. All code is open source and available on github and CRAN. Instructions for installation and use are provided at https://github.com/grunwaldlab/Microbe-ID. PMID:27602267

  8. Microbe-ID: an open source toolbox for microbial genotyping and species identification.

    PubMed

    Tabima, Javier F; Everhart, Sydney E; Larsen, Meredith M; Weisberg, Alexandra J; Kamvar, Zhian N; Tancos, Matthew A; Smart, Christine D; Chang, Jeff H; Grünwald, Niklaus J

    2016-01-01

    Development of tools to identify species, genotypes, or novel strains of invasive organisms is critical for monitoring emergence and implementing rapid response measures. Molecular markers, although critical to identifying species or genotypes, require bioinformatic tools for analysis. However, user-friendly analytical tools for fast identification are not readily available. To address this need, we created a web-based set of applications called Microbe-ID that allow for customizing a toolbox for rapid species identification and strain genotyping using any genetic markers of choice. Two components of Microbe-ID, named Sequence-ID and Genotype-ID, implement species and genotype identification, respectively. Sequence-ID allows identification of species by using BLAST to query sequences for any locus of interest against a custom reference sequence database. Genotype-ID allows placement of an unknown multilocus marker in either a minimum spanning network or dendrogram with bootstrap support from a user-created reference database. Microbe-ID can be used for identification of any organism based on nucleotide sequences or any molecular marker type and several examples are provided. We created a public website for demonstration purposes called Microbe-ID (microbe-id.org) and provided a working implementation for the genus Phytophthora (phytophthora-id.org). In Phytophthora-ID, the Sequence-ID application allows identification based on ITS or cox spacer sequences. Genotype-ID groups individuals into clonal lineages based on simple sequence repeat (SSR) markers for the two invasive plant pathogen species P. infestans and P. ramorum. All code is open source and available on github and CRAN. Instructions for installation and use are provided at https://github.com/grunwaldlab/Microbe-ID. PMID:27602267

  9. Comparison of Perinatal Risk Factors Associated with Autism Spectrum Disorder (ASD), Intellectual Disability (ID), and Co-Occurring ASD and ID

    ERIC Educational Resources Information Center

    Schieve, Laura A.; Clayton, Heather B.; Durkin, Maureen S.; Wingate, Martha S.; Drews-Botsch, Carolyn

    2015-01-01

    While studies report associations between perinatal outcomes and both autism spectrum disorder (ASD) and intellectual disability (ID), there has been little study of ASD with versus without co-occurring ID. We compared perinatal risk factors among 7547 children in the 2006-2010 Autism and Developmental Disability Monitoring Network classified as…

  10. A&M. Plot plan of administration and A&M areas. Shows relationships ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    A&M. Plot plan of administration and A&M areas. Shows relationships among administration buildings and to A&M building (TAN-607), railroad turntable. Ralph M. Parsons 902-2&3-ANP-U 3. Date: December 1952. INEEL index code no. 032-0100-00-693-106690 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID

  11. 76 FR 39770 - Interim Final Rule on Certification of Factual Information To Import Administration During...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-07

    ...'') published on February 10, 2011 (76 FR 7491). In the notice announcing the Interim Final Rule, the Department... under docket number ITA-2010-0007. To find this docket, enter the docket number in the ``Enter...

  12. ATF3 suppresses ESCC via downregulation of ID1

    PubMed Central

    Li, Jian; Yang, Zishan; Chen, Zhiuguo; Bao, Yonghua; Zhang, Huijuan; Fang, Xinhui; Yang, Wancai

    2016-01-01

    Esophageal cancer is one of the most prevalent forms of cancer and has a particularly high mortality rate due to early metastasis; however, the underlying mechanisms of its formation and progression remain unclear. The present study performed immunohistochemical analysis and observed that the expression of activating transcription factor 3 (ATF3) was reduced in esophageal squamous cell carcinoma (ESCC) in comparison with non-tumor adjacent tissues. By contrast, inhibitor of DNA binding 1 (ID1) was overexpressed in ESCC tissues, demonstrating an inverse correlation with ATF3 (P<0.01). In ESCC EC109 and KYSE450 cells lines, transfection with an ATF3-overexpression plasmid resulted in the inhibition of cell proliferation, motility and migration, which was associated with the induction of E-cadherin expression and inhibition of cyclin D1 and Twist. Notably, ATF3 exerted an inverse regulatory interaction with ID1. The results of the present study provide additional evidence of the tumor suppressive features of ATF3 and demonstrate a novel mechanism of ATF3-mediated inhibition of cancer metastasis in esophageal cancer. PMID:27602100

  13. 75 FR 42436 - Houston Pipe Line Company LP-Bammel Storage, Docket No. PR10-51-000, et. al.; Notice of Baseline...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-21

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Houston Pipe Line Company LP--Bammel Storage, Docket No. PR10-51- 000, et. al.; Notice of Baseline Filings July 14, 2010. Houston Pipe Line Company LP-- Docket No....

  14. ID4 promotes AR expression and blocks tumorigenicity of PC3 prostate cancer cells.

    PubMed

    Komaragiri, Shravan Kumar; Bostanthirige, Dhanushka H; Morton, Derrick J; Patel, Divya; Joshi, Jugal; Upadhyay, Sunil; Chaudhary, Jaideep

    2016-09-01

    Deregulation of tumor suppressor genes is associated with tumorigenesis and the development of cancer. In prostate cancer, ID4 is epigenetically silenced and acts as a tumor suppressor. In normal prostate epithelial cells, ID4 collaborates with androgen receptor (AR) and p53 to exert its tumor suppressor activity. Previous studies have shown that ID4 promotes tumor suppressive function of AR whereas loss of ID4 results in tumor promoter activity of AR. Previous study from our lab showed that ectopic ID4 expression in DU145 attenuates proliferation and promotes AR expression suggesting that ID4 dependent AR activity is tumor suppressive. In this study, we examined the effect of ectopic expression of ID4 on highly malignant prostate cancer cell, PC3. Here we show that stable overexpression of ID4 in PC3 cells leads to increased apoptosis and decreased cell proliferation and migration. In addition, in vivo studies showed a decrease in tumor size and volume of ID4 overexpressing PC3 cells, in nude mice. At the molecular level, these changes were associated with increased androgen receptor (AR), p21, and AR dependent FKBP51 expression. At the mechanistic level, ID4 may regulate the expression or function of AR through specific but yet unknown AR co-regulators that may determine the final outcome of AR function. PMID:27462022

  15. Id2 Mediates Differentiation of Labyrinthine Placental Progenitor Cell Line, SM10.

    PubMed

    Selesniemi, Kaisa; Albers, Renee E; Brown, Thomas L

    2016-07-01

    The placenta is an organ that is formed transiently during pregnancy, and appropriate placental development is necessary for fetal survival and growth. Proper differentiation of the labyrinthine layer of the placenta is especially crucial, as it establishes the fetal-maternal interface that is involved in physiological exchange processes. Although previous studies have indicated the importance of inhibitor of differentiation/inhibitor of DNA binding-2 (Id2) helix-loop-helix transcriptional regulator in mediating cell differentiation, the ability of Id2 to regulate differentiation toward the labyrinthine (transport) lineage of the placenta has yet to be determined. In the current study, we have generated labyrinthine trophoblast progenitor cells with increased (SM10-Id2) or decreased (SM10-Id2-shRNA) Id2 expression and determined the effect on TGF-β-induced differentiation. Our Id2 overexpression and knockdown analyses indicate that Id2 mediates TGF-β-induced morphological differentiation of labyrinthine trophoblast cells, as Id2 overexpression prevents differentiation and Id2 knockdown results in differentiation. Thus, our data indicate that Id2 is an important molecular mediator of labyrinthine trophoblast differentiation. An understanding of the regulators of trophoblast progenitor differentiation toward the labyrinthine lineage may offer insights into events governing pregnancy-associated disorders, such as placental insufficiency, fetal growth restriction, and preeclampsia. PMID:27168216

  16. Behavioral abnormalities and Parkinson’s-like histological changes resulting from Id2 inactivation in mice

    PubMed Central

    Havrda, Matthew C.; Paolella, Brenton R.; Ward, Nora M.; Holroyd, Kathryn B.

    2013-01-01

    SUMMARY Characterizing dopaminergic neuronal development and function in novel genetic animal models might uncover strategies for researchers to develop disease-modifying treatments for neurologic disorders. Id2 is a transcription factor expressed in the developing central nervous system. Id2−/− mice have fewer dopaminergic neurons in the olfactory bulb and reduced olfactory discrimination, a pre-clinical marker of Parkinson’s disease. Here, we summarize behavioral, histological and in vitro molecular biological analyses to determine whether midbrain dopaminergic neurons are affected by Id2 loss. Id2−/− mice were hyperactive at 1 and 3 months of age, but by 6 months showed reduced activity. Id2−/− mice showed age-dependent histological alterations in dopaminergic neurons of the substantia nigra pars compacta (SNpC) associated with changes in locomotor activity. Reduced dopamine transporter (DAT) expression was observed at early ages in Id2−/− mice and DAT expression was dependent on Id2 expression in an in vitro dopaminergic differentiation model. Evidence of neurodegeneration, including activated caspase-3 and glial infiltration, were noted in the SNpC of older Id2−/− mice. These findings document a novel role for Id2 in the maintenance of midbrain dopamine neurons. The Id2−/− mouse should provide unique opportunities to study the progression of neurodegenerative disorders involving the dopamine system. PMID:23264561

  17. Id-1 as a molecular target in therapy for breast cancer cell invasion and metastasis

    NASA Astrophysics Data System (ADS)

    Fong, Sylvia; Itahana, Yoko; Sumida, Tomoki; Singh, Jarnail; Coppe, Jean-Philippe; Liu, Yong; Richards, Peter C.; Bennington, James L.; Lee, Nancy M.; Debs, Robert J.; Desprez, Pierre-Yves

    2003-11-01

    Mammary epithelial cells constitutively expressing Id-1 protein are unable to differentiate, acquire the ability to proliferate, and invade the extracellular matrix. In addition, Id-1 is aberrantly over-expressed in aggressive and metastatic breast cancer cells, as well as in human breast tumor biopsies from infiltrating carcinomas, suggesting Id-1 might be an important regulator of breast cancer progression. We show that human metastatic breast cancer cells become significantly less invasive in vitro and less metastatic in vivo when Id-1 is down-regulated by stable transduction with antisense Id-1. Expression of the matrix metalloproteinase MT1-MMP is decreased in proportion to the decrease in Id-1 protein levels, representing a potential mechanism for the reduction of invasiveness. Further, to more accurately recapitulate the biology of and potential therapeutic approaches to tumor metastasis, we targeted Id-1 expression systemically in tumor-bearing mice by using a nonviral approach. We demonstrate significant reduction of both Id-1 and MT1-MMP expressions as well as the metastatic spread of 4T1 breast cancer cells in syngeneic BALB/c mice. In conclusion, our studies have identified Id-1 as a critical regulator of breast cancer progression and suggest the feasibility of developing novel therapeutic approaches to target Id-1 expression to reduce breast cancer metastasis in humans.

  18. Clinical significance of up-regulated ID1 expression in Chinese de novo acute myeloid leukemia

    PubMed Central

    Zhou, Jing-Dong; Yang, Lei; Zhu, Xiao-Wen; Wen, Xiang-Mei; Yang, Jing; Guo, Hong; Chen, Qin; Yao, Dong-Ming; Ma, Ji-Chun; Lin, Jiang; Qian, Jun

    2015-01-01

    To investigate the clinical significance of ID1 expression in Chinese de novo AML patients. Real-time quantitative PCR was carried out to detect the status of ID1 expression in 102 de novo AML patients and 28 controls. ID1 transcript level was significantly increased in AML compared to normal controls (p=0.029). The age in the patients with high ID1 expression is significantly older than in those with low ID1 expression (p=0.044). ID1 overexpression occurred with the highest frequency in the patients with poor karyotype (7/7, 100%), lower frequency in the patients with intermediate karyotype (28/60, 47%), and the lowest frequency in the patients with favorable karyotype (12/31, 39%). Both whole AML and non-M3 patients with high ID1 expression had significantly lower rate of complete remission than those with low ID1 expression (p=0.007 and 0.038). ID1 high-expressed patients showed significantly shorter overall survival (OS) than ID1 low-expressed patients in both whole AML and non-M3 according to Kaplan-Meier analysis (p=0.007 and 0.040). However, multivariate analysis indicated that ID1 overexpression was not an independent risk factor in both whole AML and non-M3 patients. However, the adverse impact of ID1 overexpression on outcome was revealed by both Kaplan-Meier analysis and multivariate analysis in the non-M3 patients less than 60 years old. Our study reveals that ID1 overexpression may be associated with higher risk karyotype classification and act as an independent risk factor in young non-M3 patients. PMID:26191235

  19. Possibility of splitting APS dipoles to make more ID straights.

    SciTech Connect

    Borland, M.; Accelerator Systems Division

    2009-09-29

    Recently we were asked by Ercan Alp if it would be possible to use three dipoles in an APS sector to make a second ID beamline possible, much as was done for the APSx3 design. We've looked at this to see what might be possible without making too many changes to the ring. Ideally, we'd like to simply replace the one dipole with two shorter dipoles with an insertion device in the middle. Of course, we'd like to make the ID as long as possible, subject to limits on dipole strength, accelerator optics, and emittance. We used elegant [3] to match an insertion device consisting of two sectors. The second (BM) dipole of the first sector was split at the 1/8th point in terms of both angle and length. A zero length drift space was inserted that could then be stretched to create room for an ID. To simplify matters somewhat at this stage, we constrained the length of the two dipoles plus the drift to equal the original dipole length. I.e., we ignored for now the difference between arc and straight length. Our desire is to make the drift space as long as possible. If we limit the dipole field B to 2T, then the total length of the dipoles must be greater than L{sub min} = H{theta}/B, where H = 23.35Tm is the rigidity and {theta} = {pi}/40-0.001 is the total angle. L{sub min} is therefore 0.91 m, and hence the drift space can at most be 2.15 m. At least one, perhaps both, of the short dipoles will end up in a high-dispersion region, which will lead to emittance growth. Hence, we didn't want to drive the length too far and decided to limit it to 2 m, approximately the same as in the APSx3 lattice. Hence, as in that lattice we should be able to accommodate a 1-m-long insertion device. In addition to stretching the drift space, we allowed all twenty quadrupoles in the sectors to vary. Initial constraints included less than 20% deviation in lattice functions at the middle ID straight and less than 0.001% deviation in the final ID straight (to get a good match to the rest of the ring

  20. 6 CFR 37.29 - Prohibition against holding more than one REAL ID card or more than one driver's license.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... ID card or more than one driver's license. 37.29 Section 37.29 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum... REAL ID card or more than one driver's license. (a) An individual may hold only one REAL ID card....

  1. 6 CFR 37.29 - Prohibition against holding more than one REAL ID card or more than one driver's license.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... ID card or more than one driver's license. 37.29 Section 37.29 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum... REAL ID card or more than one driver's license. (a) An individual may hold only one REAL ID card....

  2. 6 CFR 37.29 - Prohibition against holding more than one REAL ID card or more than one driver's license.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... ID card or more than one driver's license. 37.29 Section 37.29 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum... REAL ID card or more than one driver's license. (a) An individual may hold only one REAL ID card....

  3. 6 CFR 37.29 - Prohibition against holding more than one REAL ID card or more than one driver's license.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... ID card or more than one driver's license. 37.29 Section 37.29 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum... REAL ID card or more than one driver's license. (a) An individual may hold only one REAL ID card....

  4. An improved smaller biotin ligase for BioID proximity labeling

    PubMed Central

    Kim, Dae In; Jensen, Samuel C.; Noble, Kyle A.; KC, Birendra; Roux, Kenneth H.; Motamedchaboki, Khatereh; Roux, Kyle J.

    2016-01-01

    The BioID method uses a promiscuous biotin ligase to detect protein–protein associations as well as proximate proteins in living cells. Here we report improvements to the BioID method centered on BioID2, a substantially smaller promiscuous biotin ligase. BioID2 enables more-selective targeting of fusion proteins, requires less biotin supplementation, and exhibits enhanced labeling of proximate proteins. Thus BioID2 improves the efficiency of screening for protein–protein associations. We also demonstrate that the biotinylation range of BioID2 can be considerably modulated using flexible linkers, thus enabling application-specific adjustment of the biotin-labeling radius. PMID:26912792

  5. Image processing by Intensity-Dependent Spread (IDS)

    NASA Technical Reports Server (NTRS)

    Cornsweet, Tom N.

    1989-01-01

    As retinal illuminance is lowered, the human visual system integrates the effects of photon absorptions over larger areas and longer times. A theory of the process that might underlie these changes is called Intensity-Dependent Spread (IDS). Each input point gives rise to a pattern of excitation that spreads to a region of the total excitation it sees. The unique aspect of the theory is the assumption that, although the amplitude of the excitation pattern at its center increases with input illuminance, its width decreases in such a way that its volume remains constant. Application of this theory to image processing reveals that it displays a number of unexpected and potentially useful properties. Among them are edge enhancement and independence from scene illumination.

  6. ID-Check: Online Concealed Information Test Reveals True Identity.

    PubMed

    Verschuere, Bruno; Kleinberg, Bennett

    2016-01-01

    The Internet has already changed people's lives considerably and is likely to drastically change forensic research. We developed a web-based test to reveal concealed autobiographical information. Initial studies identified a number of conditions that affect diagnostic efficiency. By combining these moderators, this study investigated the full potential of the online ID-check. Participants (n = 101) tried to hide their identity and claimed a false identity in a reaction time-based Concealed Information Test. Half of the participants were presented with personal details (e.g., first name, last name, birthday), whereas the others only saw irrelevant details. Results showed that participants' true identity could be detected with high accuracy (AUC = 0.98; overall accuracy: 86-94%). Online memory detection can reliably and validly detect whether someone is hiding their true identity. This suggests that online memory detection might become a valuable tool for forensic applications. PMID:26390033

  7. The Implementation of C-ID, R2D2 Model on Learning Reading Comprehension

    ERIC Educational Resources Information Center

    Rayanto, Yudi Hari; Rusmawan, Putu Ngurah

    2016-01-01

    The purposes of this research are to find out, (1) whether C-ID, R2D2 model is effective to be implemented on learning Reading comprehension, (2) college students' activity during the implementation of C-ID, R2D2 model on learning Reading comprehension, and 3) college students' learning achievement during the implementation of C-ID, R2D2 model on…

  8. Underground Storage Tank Integrated Demonstration (UST-ID). Technology summary

    SciTech Connect

    Not Available

    1994-02-01

    The DOE complex currently has 332 underground storage tanks (USTs) that have been used to process and store radioactive and chemical mixed waste generated from weapon materials production. Very little of the over 100 million gallons of high-level and low-level radioactive liquid waste has been treated and disposed of in final form. Two waste storage tank design types are prevalent across the DOE complex: single-shell wall and double-shell wall designs. They are made of stainless steel, concrete, and concrete with carbon steel liners, and their capacities vary from 5000 gallons (19 m{sup 3}) to 10{sup 6} gallons (3785 m{sup 3}). The tanks have an overburden layer of soil ranging from a few feet to tens of feet. Responding to the need for remediation of tank waste, driven by Federal Facility Compliance Agreements (FFCAs) at all participating sites, the Underground Storage Tank Integrated Demonstration (UST-ID) Program was created by the US DOE Office of Technology Development in February 1991. Its mission is to focus the development, testing, and evaluation of remediation technologies within a system architecture to characterize, retrieve, treat to concentrate, and dispose of radioactive waste stored in USTs at DOE facilities. The ultimate goal is to provide safe and cost-effective solutions that are acceptable to the public and the regulators. The UST-ID has focused on five DOE locations: the Hanford Site, which is the host site, in Richland, Washington; the Fernald Site in Fernald, Ohio; the Idaho National Engineering Laboratory near Idaho Falls, Idaho; the Oak Ridge Reservation in Oak Ridge, Tennessee, and the Savannah River Site in Savannah River, South Carolina.

  9. DeID - a data sharing tool for neuroimaging studies.

    PubMed

    Song, Xuebo; Wang, James; Wang, Anlin; Meng, Qingping; Prescott, Christian; Tsu, Loretta; Eckert, Mark A

    2015-01-01

    Funding institutions and researchers increasingly expect that data will be shared to increase scientific integrity and provide other scientists with the opportunity to use the data with novel methods that may advance understanding in a particular field of study. In practice, sharing human subject data can be complicated because data must be de-identified prior to sharing. Moreover, integrating varied data types collected in a study can be challenging and time consuming. For example, sharing data from structural imaging studies of a complex disorder requires the integration of imaging, demographic and/or behavioral data in a way that no subject identifiers are included in the de-identified dataset and with new subject labels or identification values that cannot be tracked back to the original ones. We have developed a Java program that users can use to remove identifying information in neuroimaging datasets, while still maintaining the association among different data types from the same subject for further studies. This software provides a series of user interaction wizards to allow users to select data variables to be de-identified, implements functions for auditing and validation of de-identified data, and enables the user to share the de-identified data in a single compressed package through various communication protocols, such as FTPS and SFTP. DeID runs with Windows, Linux, and Mac operating systems and its open architecture allows it to be easily adapted to support a broader array of data types, with the goal of facilitating data sharing. DeID can be obtained at http://www.nitrc.org/projects/deid. PMID:26441500

  10. Fake ID ownership and heavy drinking in underage college students: prospective findings.

    PubMed

    Martinez, Julia A; Rutledge, Patricia C; Sher, Kenneth J

    2007-06-01

    The authors examined the ownership of false identification (fake ID) for the purpose of obtaining alcohol and the relation of fake ID ownership to heavy drinking in a longitudinal sample of college students under 21 years of age. A sample of 3,720 undergraduates was assessed the summer prior to college entrance and during the 4 semesters comprising freshman and sophomore years. Regression analyses were used to estimate bidirectional relations between consumption and fake ID ownership. Sex, Greek membership, and prior drinking were controlled. Results showed that fake ID ownership increased over time (12.5% pre-college to 32.2% fourth semester) and that Greek members were more likely than others to own fake IDs. Fake ID ownership predicted concurrent and next-semester heavy drinking with increasing strength over time. Also, the acquisition (onset) of fake ID ownership at each time point was predicted by previous-semester consumption. When traditional, robust risk factors of consumption are controlled, fake ID ownership meaningfully relates to heavy drinking in college. It thus presents a significant public health problem, addressable through training for alcohol servers and retailers, punitive measures toward fake ID owners, and other possible interventions. PMID:17563142

  11. Silencing of Id2 Alleviates Chronic Neuropathic Pain Following Chronic Constriction Injury.

    PubMed

    Jiang, Liuming; Wu, Qun; Yang, Tao

    2016-05-01

    Inhibitor of DNA binding/differentiation 2 (Id2) belongs to a helix-loop-helix family of proteins. Recent studies have showed that Id2 plays a pivotal role in neuronal survival and neuroprotection. However, under neuropathic pain conditions, the role of Id2 is still unclear. In this study, we investigated the effect of Id2 on neuropathic pain in a rat chronic constriction injury (CCI) model. Our results demonstrated that Id2 was upregulated in the dorsal root ganglion (DRG) in a CCI rat in a time-dependent manner. Intrathecal short-hairpin RNA (shRNA)-Id2 attenuates mechanical allodynia and thermal hyperalgesia in CCI rats, and inhibits the expression of TNF-α and IL-1β in the DRG in CCI rats. Furthermore, knockdown of Id2 reduces the expression of NF-κB p65 in the DRG of CCI rats. Taken together, our findings suggest that knockdown of Id2 may alleviate neuropathic pain by inhibiting the NF-κB activation to inhibit the production of pro-inflammatory mediators. Therefore, Id2 may provide an important target of neuropathic pain treatment. PMID:26768262

  12. Id expression in amphioxus and lamprey highlights the role of gene cooption during neural crest evolution

    NASA Technical Reports Server (NTRS)

    Meulemans, Daniel; McCauley, David; Bronner-Fraser, Marianne

    2003-01-01

    Neural crest cells are unique to vertebrates and generate many of the adult structures that differentiate them from their closest invertebrate relatives, the cephalochordates. Id genes are robust markers of neural crest cells at all stages of development. We compared Id gene expression in amphioxus and lamprey to ask if cephalochordates deploy Id genes at the neural plate border and dorsal neural tube in a manner similar to vertebrates. Furthermore, we examined whether Id expression in these cells is a basal vertebrate trait or a derived feature of gnathostomes. We found that while expression of Id genes in the mesoderm and endoderm is conserved between amphioxus and vertebrates, expression in the lateral neural plate border and dorsal neural tube is a vertebrate novelty. Furthermore, expression of lamprey Id implies that recruitment of Id genes to these cells occurred very early in the vertebrate lineage. Based on expression in amphioxus we postulate that Id cooption conferred sensory cell progenitor-like properties upon the lateral neurectoderm, and pharyngeal mesoderm-like properties upon cranial neural crest. Amphioxus Id expression is also consistent with homology between the anterior neurectoderm of amphioxus and the presumptive placodal ectoderm of vertebrates. These observations support the idea that neural crest evolution was driven in large part by cooption of multipurpose transcriptional regulators from other tissues and cell types.

  13. Expression of Id-1 and VEGF in non-small cell lung cancer

    PubMed Central

    Kim, Mee-Seon; Park, Tae-In; Lee, Yu-Mi; Jo, Young-Min; Kim, Sunzoo

    2013-01-01

    Angiogenesis is essential for invasive tumor growth and metastasis. Bevacizumab has been widely used for the treatment of non-small cell lung cancer (NSCLC). Various studies clearly demonstrate the relevance of Id-1 and VEGF in angiogenesis. The aim of this study was to establish the role of Id-1 expression in tumor progression and angiogenesis in relation to VEGF in NSCLC. Seventy five patients underwent surgery for lung cancers. The expressions of Id-1 and VEGF in NSCLC samples were determined by immunohistochemistry. Expression of Id-1 and VEGF showed a close correlation in NSCLC (p < 0.001). In addition, Id-1 strong expression group showed high incidence of metastasis in multivariate analysis (p = 0.028). Id-1 strong expression group had short metastasis-free survival (p = 0.008) and short recurrence-free survival (p = 0.027). Strong Id-1 expression in NSCLC had a poor prognosis in association with VEGF expression. Id-1 may function in tumor growth and progression via angiogenesis. Therefore, Id-1 is considered to be a candidate for new therapeutic target and a prognostic factor in NSCLC. PMID:24133588

  14. Idaho National Laboratory Supervisory Control and Data Acquisition Intrusion Detection System (SCADA IDS)

    SciTech Connect

    Jared Verba; Michael Milvich

    2008-05-01

    Current Intrusion Detection System (IDS) technology is not suited to be widely deployed inside a Supervisory, Control and Data Acquisition (SCADA) environment. Anomaly- and signature-based IDS technologies have developed methods to cover information technology-based networks activity and protocols effectively. However, these IDS technologies do not include the fine protocol granularity required to ensure network security inside an environment with weak protocols lacking authentication and encryption. By implementing a more specific and more intelligent packet inspection mechanism, tailored traffic flow analysis, and unique packet tampering detection, IDS technology developed specifically for SCADA environments can be deployed with confidence in detecting malicious activity.

  15. 75 FR 80854 - Toshiba Corporation; Acceptance for Docketing of an Application for Renewal of the U.S. Advanced...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-23

    ... availability of this application was previously published in the Federal Register (75 FR 71744) on November 24... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Toshiba Corporation; Acceptance for Docketing of an Application for Renewal of the U.S....

  16. 75 FR 33653 - Exelon Nuclear Texas Holdings, LLC (Exelon) Acceptance for Docketing of an Application for an...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-14

    ... was previously published in the Federal Register (75 FR 22434: April 28, 2010). The applicant... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Exelon Nuclear Texas Holdings, LLC (Exelon) Acceptance for Docketing of an Application for...

  17. 78 FR 35990 - All Operating Boiling-Water Reactor Licensees With Mark I And Mark II Containments; Docket Nos...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-14

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION All Operating Boiling-Water Reactor Licensees With Mark I And Mark II Containments; Docket Nos. (As Shown In Attachment 1), License Nos. (As Shown In Attachment 1), EA-13-109; Order Modifying Licenses With Regard to Reliable Hardened...

  18. 48 CFR 6101.2 - Filing cases; time limits for filing; notice of docketing; consolidation [Rule 2].

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 7 2012-10-01 2012-10-01 false Filing cases; time limits... Filing cases; time limits for filing; notice of docketing; consolidation . (a) Filing cases. Filing of a... machine number, and e-mail address, if available, of the contracting officer whose decision is...

  19. 48 CFR 6101.2 - Filing cases; time limits for filing; notice of docketing; consolidation [Rule 2].

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 7 2013-10-01 2012-10-01 true Filing cases; time limits... Filing cases; time limits for filing; notice of docketing; consolidation . (a) Filing cases. Filing of a... machine number, and e-mail address, if available, of the contracting officer whose decision is...

  20. 48 CFR 6101.2 - Filing cases; time limits for filing; notice of docketing; consolidation [Rule 2].

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 7 2011-10-01 2011-10-01 false Filing cases; time limits... Filing cases; time limits for filing; notice of docketing; consolidation . (a) Filing cases. Filing of a... machine number, and e-mail address, if available, of the contracting officer whose decision is...

  1. 48 CFR 6101.2 - Filing cases; time limits for filing; notice of docketing; consolidation [Rule 2].

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 7 2014-10-01 2014-10-01 false Filing cases; time limits... Filing cases; time limits for filing; notice of docketing; consolidation . (a) Filing cases. Filing of a... machine number, and e-mail address, if available, of the contracting officer whose decision is...

  2. 21 CFR 10.20 - Submission of documents to Division of Dockets Management; computation of time; availability for...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Management; computation of time; availability for public disclosure. 10.20 Section 10.20 Food and Drugs FOOD... Management; computation of time; availability for public disclosure. (a) A submission to the Division of Dockets Management of a petition, comment, objection, notice, compilation of information, or any...

  3. 10 CFR 2.621 - Acceptance and docketing of application for early review of site suitability issues in a combined...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Cfr Part 52 § 2.621 Acceptance and docketing of application for early review of site suitability... of site suitability issues in a combined license proceeding. 2.621 Section 2.621 Energy NUCLEAR... Procedures Applicable to Early Partial Decisions on Site Suitability Issues in Connection With an...

  4. 76 FR 9612 - GE Hitachi Nuclear Energy; Acceptance for Docketing of an Application for Renewal of the U.S...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-18

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION GE Hitachi Nuclear Energy; Acceptance for Docketing of an Application for Renewal of the U.S. Advanced Boiling Water Reactor Design Certification On December 7, 2010, GE Hitachi Nuclear Energy (GEH) submitted an application to the U.S....

  5. 10 CFR 2.603 - Acceptance and docketing of application for early review of site suitability issues in a...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... processing, the Director of the Office of New Reactors or the Director of the Office of Nuclear Reactor... Nuclear Reactor Regulation, as appropriate, that they are complete. (c) If part one of the application is docketed, the Director of the Office of New Reactors or the Director of the Office of Nuclear...

  6. 75 FR 43566 - University of Maryland; Notice of Acceptance for Docketing and Opportunity for Hearing on the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-26

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION University of Maryland; Notice of Acceptance for Docketing and Opportunity for Hearing on the Application Regarding Renewal of Facility Operating License for an Additional 20-Year Period for the Maryland University Training Reactor and...

  7. 10 CFR 2.603 - Acceptance and docketing of application for early review of site suitability issues in a...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Acceptance and docketing of application for early review of site suitability issues in a construction permit proceeding. 2.603 Section 2.603 Energy NUCLEAR REGULATORY COMMISSION RULES OF PRACTICE FOR DOMESTIC LICENSING PROCEEDINGS AND ISSUANCE OF ORDERS Additional Procedures Applicable to Early...

  8. 10 CFR 2.621 - Acceptance and docketing of application for early review of site suitability issues in a combined...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Acceptance and docketing of application for early review of site suitability issues in a combined license proceeding. 2.621 Section 2.621 Energy NUCLEAR REGULATORY COMMISSION RULES OF PRACTICE FOR DOMESTIC LICENSING PROCEEDINGS AND ISSUANCE OF ORDERS Additional Procedures Applicable to Early...

  9. Ebf1-mediated down-regulation of Id2 and Id3 is essential for specification of the B cell lineage

    PubMed Central

    Thal, Melissa A.; Carvalho, Thiago L.; He, Ti; Kim, Hyung-Gyoon; Gao, Hua; Hagman, James; Klug, Christopher A.

    2009-01-01

    Gene knockout experiments in mice have suggested a hierarchical model of early B cell commitment wherein E2A proteins (E47 and E12) activate early B cell factor (Ebf1), which in turn activates expression of the B cell commitment factor, Pax5. In IL-7 receptor alpha (IL-7Rα) knockout mice, B cell development is blocked before B-lineage commitment at the prepro-B cell stage in adult animals. In IL-7Rα−/− prepro-B cells, E47 is expressed and yet is insufficient to transcriptionally activate the putative downstream target gene, Ebf1. In this study, we show that further increases of E47 expression in IL-7Rα−/− prepro-B cells fails to activate Ebf1, but rather leads to a dramatic induction of the E2A inhibitory factors, Id2 and Id3. In contrast, enforced expression of Ebf1 in IL-7Rα−/− bone marrow potently down-regulates Id2 and Id3 mRNA expression and restores B cell differentiation in vivo. Down-regulation of both Id2 and Id3 during B cell specification is essential in that overexpression of either Id2 or Id3 in wild-type bone marrow blocks B cell specification at the prepro-B cell stage. Collectively, these studies suggest a model where Ebf1 induction specifies the B cell fate by dramatically increasing activity of E47 at the posttranslational level. PMID:19122139

  10. Conventional Chiralpak ID vs. capillary Chiralpak ID-3 amylose tris-(3-chlorophenylcarbamate)-based chiral stationary phase columns for the enantioselective HPLC separation of pharmaceutical racemates.

    PubMed

    Ahmed, Marwa; Gwairgi, Marina; Ghanem, Ashraf

    2014-11-01

    A comparative enantioselective analysis using immobilized amylose tris-(3-chlorophenylcarbamate) as chiral stationary phase in conventional high-performance liquid chromatography (HPLC) with Chiralpak ID (4.6 mm ID × 250 mm, 5 µm silica gel) and micro-HPLC with Chiralpak ID-3 (0.30 mm ID × 150 mm, 3 µm silica gel) was conducted. Pharmaceutical racemates of 12 pharmacological classes, namely, α- and β-blockers, anti-inflammatory drugs, antifungal drugs, dopamine antagonists, norepinephrine-dopamine reuptake inhibitors, catecholamines, sedative hypnotics, diuretics, antihistaminics, anticancer drugs, and antiarrhythmic drugs were screened under normal phase conditions. The effect of an organic modifier on the analyte retentions and enantiomer recognition was investigated. Baseline separation was achieved for 1-acenaphthenol, carprofen, celiprolol, cizolirtine carbinol, miconazole, tebuconazole, 4-hydroxy-3-methoxymandelic acid, 1-indanol, 1-(2-chlorophenyl)ethanol, 1-phenyl-2-propanol, flavanone, 6-hydroxyflavanone, 4-bromogluthethimide, and pentobarbital on the 4.6 mm ID packed with a 5 µm silica column using conventional HPLC. Nonetheless, baseline separation was achieved for aminoglutethimide, naftopidil, and thalidomide on the 0.3 mm ID packed with a 3 µm silica capillary column. PMID:25271972

  11. FHL2 interacts with and acts as a functional repressor of Id2 in human neuroblastoma cells

    PubMed Central

    Han, Weidong; Wu, Zhiqiang; Zhao, Yali; Meng, Yuanguang; Si, Yiling; Yang, Jie; Fu, Xiaobing; Yu, Li

    2009-01-01

    Inhibitor of differentiation 2 (Id2) is a natural inhibitor of the basic helix–loop–helix transcription factors. Although Id2 is well known to prevent differentiation and promote cell-cycle progression and tumorigenesis, the molecular events that regulate Id2 activity remain to be investigated. Here, we identified that Four-and-a-half LIM-only protein 2 (FHL2) is a novel functional repressor of Id2. Moreover, we demonstrated that FHL2 can directly interact with all members of the Id family (Id1–4) via an N-terminal loop–helix structure conserved in Id proteins. FHL2 antagonizes the inhibitory effect of Id proteins on basic helix–loop–helix protein E47-mediated transcription, which was abrogated by the deletion mutation of Ids that disrupted their interaction with FHL2. We also showed a competitive nature between FHL2 and E47 for binding Id2, whereby FHL2 prevents the formation of the Id2–E47 heterodimer, thus releasing E47 to DNA and restoring its transcriptional activity. FHL2 expression was remarkably up-regulated during retinoic acid-induced differentiation of neuroblastoma cells, during which the expression of Id2 was opposite to that. Ectopic FHL2 expression in neuroblastoma cells markedly reduces the transcriptional and cell-cycle promoting functions of Id2. Altogether, these results indicate that FHL2 is an important repressor of the oncogenic activity of Id2 in neuroblastoma cells. PMID:19417068

  12. Security and Privacy Improvements for the Belgian eID Technology

    NASA Astrophysics Data System (ADS)

    Verhaeghe, Pieter; Lapon, Jorn; de Decker, Bart; Naessens, Vincent; Verslype, Kristof

    The Belgian Electronic Identity Card enables Belgian citizens to prove their identity digitally and to sign electronic documents. At the end of 2009, every Belgian citizen older than 12 years will have such an eID card. In the future, usage of the eID card may be mandatory. However, irresponsible use of the card may cause harm to individuals.

  13. 13. TIP TOP MINE. WOODEN STRUCTURE VISIBLE IN ID31C12 ISIN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. TIP TOP MINE. WOODEN STRUCTURE VISIBLE IN ID-31-C-12 ISIN UPPER LEFT. ALSO VISIBLE ARE TWO VERTICALLY BURIED LOGS ASSOCIATED WITH CABLES, ONE OF WHICH IS VISIBLE IN LOWER RIGHT. CAMERA POINTED SOUTH. - Florida Mountain Mining Sites, Tip Top Mine, West face Florida Mountain, approximately 150 feet below summit, Silver City, Owyhee County, ID

  14. Wicked ID: Conceptual Framework for Considering Instructional Design as a Wicked Problem

    ERIC Educational Resources Information Center

    Becker, Katrin

    2007-01-01

    The process of instructional design has parallels in other design disciplines. Software design is one that has experienced intense attention in the last 30 or so years, and many lessons learned there can be applied to ID. Using software design as a springboard, this concept paper seeks to propose a new approach to ID. It suggests that…

  15. 77 FR 55486 - Agency Information Collection Activities: Importer ID Input Record

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-10

    ... Federal Register (76 FR 37696) on June 22, 2012, allowing for a 60-day comment period. This notice allows... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Importer ID Input... accordance with the Paperwork Reduction Act: Importer ID Input Record (CBP Form 5106). This is a...

  16. 78 FR 20135 - Notice of Temporary Closure on Public Lands in Boise County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-03

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE INTERIOR Bureau of Land Management Notice of Temporary Closure on Public Lands in Boise County, ID AGENCY: Bureau... 83705 and on the BLM- Idaho Web site: http://www.blm.gov/id/st/en/advisories-closures.html . The...

  17. 76 FR 56145 - Clearwater National Forest; ID; Upper Lochsa Land Exchange EIS

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-12

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF AGRICULTURE Forest Service Clearwater National Forest; ID; Upper Lochsa Land Exchange EIS ACTION: Notice of Intent To... is: Rick Brazell, Forest Supervisor, Clearwater National Forest, 12730 HWY 12, Orofino, ID 83544....

  18. 77 FR 33794 - True Product ID, Inc.; Order of Suspension of Trading

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-07

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION True Product ID, Inc.; Order of Suspension of Trading June 5, 2012. It appears to the Securities... securities of True Product ID, Inc. (``True Product'') because it has not filed a periodic report since...

  19. 17 CFR 259.602 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... List of CFR Sections Affected, which appears in the Finding Aids section of the printed volume and at... 17 Commodity and Securities Exchanges 3 2011-04-01 2011-04-01 false Form ID, uniform application... OF 1935 Forms for Electronic Filing § 259.602 Form ID, uniform application for access codes to...

  20. Rethinking Social Network Assessment for Students with Intellectual Disabilities (ID) in Postsecondary Education

    ERIC Educational Resources Information Center

    Eisenman, Laura T.; Farley-Ripple, Elizabeth; Culnane, Mary; Freedman, Brian

    2013-01-01

    Social networks of persons with intellectual disabilities (ID) have been characterized as smaller and less diverse than those of typical peers. Advocates have focused on strengthening those social networks by expanding circles of social support, protection, and friendship. As young adults with ID experience increasing levels of community…

  1. Matching the Judgmental Task with Standard Setting Panelist Expertise: The Item-Descriptor (ID) Matching Method

    ERIC Educational Resources Information Center

    Ferrara, Steve; Perie, Marianne; Johnson, Eugene

    2008-01-01

    Psychometricians continue to introduce new approaches to setting cut scores for educational assessments in an attempt to improve on current methods. In this paper we describe the Item-Descriptor (ID) Matching method, a method based on IRT item mapping. In ID Matching, test content area experts match items (i.e., their judgments about the knowledge…

  2. Microvascular lesions by estrogen-induced ID3: its implications in cerebral & cardiorenal vascular disease

    PubMed Central

    Das, Jayanta K.; Felty, Quentin

    2014-01-01

    Severe symptoms of cerebral and cardiorenal vascular diseases can be triggered when cerebral, coronary, or glomerular arterioles grow inappropriately as a result of abnormal cell proliferation. The risk factor(s) and molecular mechanisms responsible for microvascular lesion formation are largely unknown. Although controversial, both animal and epidemiological studies have shown that estrogen increases the risk of stroke which may be due to microvascular lesions. Since microvascular diseases are characterized by excessive vessel growth, it is plausible that estrogen-induced neovascularization contributes to the growth of microvascular lesions. We present evidence for how ID3 overexpression in endothelial cells contributes to the development of an estrogen-induced neovascular phenotype with an additional focus on Pyk2 kinase. Our data showed that ID3 overexpression increased neovascularization, cell migration, and spheroid growth of human cerebral microvascular endothelial cells, hCMEC/D3. ID3 overexpressing cells showed significant estrogen-induced G2/M phase transition. Estrogen treatment increased both ID3 phosphorylation and total protein that was inhibited by tamoxifen; and Pyk2 mediated estrogen-induced ID3 mRNA expression. These findings suggest that Pyk2 signals ID3 expression and ID3 is necessary for estrogen-induced neovascularization in hCMEC/D3 cells. A better understanding of how microvascular lesions depend on ID3 may open new avenues for prevention and treatment of neurological diseases. PMID:25129100

  3. Integrated Design System (IDS) Tools for the Spacecraft Aeroassist/Entry Vehicle Design Process

    NASA Technical Reports Server (NTRS)

    Olynick, David; Braun, Robert; Langhoff, Steven R. (Technical Monitor)

    1997-01-01

    The definition of the Integrated Design System technology focus area as presented in the NASA Information Technology center of excellence strategic plan is described. The need for IDS tools in the aeroassist/entry vehicle design process is illustrated. Initial and future plans for spacecraft IDS tool development are discussed.

  4. 6 CFR 37.25 - Renewal of REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 6 Domestic Security 1 2010-01-01 2010-01-01 false Renewal of REAL ID driver's licenses and identification cards. 37.25 Section 37.25 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.25 Renewal...

  5. 75 FR 47515 - Identification (ID) Cards for Members of the Uniformed Services, Their Dependents, and Other...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-06

    ...The Department of Defense (DoD) proposes to establish policy, assign responsibilities, and provide procedures for the issuing of distinct DoD ID cards. The ID cards shall be issued to uniformed service members, their dependents, and other eligible individuals and will be used as proof of identity and DoD...

  6. 6 CFR 37.23 - Reissued REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 6 Domestic Security 1 2010-01-01 2010-01-01 false Reissued REAL ID driver's licenses and identification cards. 37.23 Section 37.23 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.23 Reissued...

  7. VizieR Online Data Catalog: Catalogue of bright IDS stars (Lipaeva+, 2014)

    NASA Astrophysics Data System (ADS)

    Lipaeva, N. A.; Sementsov, V. N.; Malkov, O.

    2014-11-01

    A new catalogue of bright binary stars is presented. The catalogue includes bright IDS systems and bright spectroscopic binaries. Besides IDS data (coordinates, relative positions, magnitudes and spectral classification) the catalogue contains extensive cross-identification and comments for 27452 systems. The catalogue is complete to magnitudes of 9mag, but contains stars down to about 16mag. (2 data files).

  8. E and Id proteins influence invariant Natural Killer T cell sublineage differentiation and proliferation

    PubMed Central

    D'Cruz, Louise M.; Stradner, Martin H.; Yang, Cliff Y.; Goldrath, Ananda W.

    2014-01-01

    Disease outcome is known to be influenced by defined subsets of invariant Natural Killer T (iNKT) cells residing in distinct locations within peripheral tissue. However, the factors governing the development of these unique iNKT sublineages during thymic development are unknown. Here we explored the mechanism by which E protein transcription factors and their negative regulators, the Id proteins control the development of iNKT sublineages after positive selection. We found that E proteins directly bound the PLZF promoter and were required for expression of this lineage-defining transcription factor and for the maturation and expansion of thymic iNKT cells. Moreover, expression of the negative regulators of E proteins, Id2 and Id3, defined distinct iNKT cell sublineages. Id3 was expressed in PLZFhigh NKT2 cells and loss of Id3 allowed for increased thymic iNKT cell expansion and abundance of the PLZF+ NKT2 sublineage. Id2 was expressed in TBET+ NKT1 cells and both Id proteins were required for the formation of this sublineage. Thus, we provide insight into E and Id protein regulation of iNKT cell proliferation and differentiation to specific sublineages during development in the thymus. PMID:24470501

  9. Regulation of Id2 expression by CCAAT/enhancer binding protein β

    PubMed Central

    Karaya, Kazuhiro; Mori, Seiichi; Kimoto, Hisashi; Shima, Yoko; Tsuji, Yoshihito; Kurooka, Hisanori; Akira, Shizuo; Yokota, Yoshifumi

    2005-01-01

    Mice deficient for Id2, a negative regulator of basic helix–loop–helix (bHLH) transcription factors, exhibit a defect in lactation due to impaired lobuloalveolar development during pregnancy, similar to the mice lacking the CCAAT enhancer binding protein (C/EBP) β. Here, we show that Id2 is a direct target of C/EBPβ. Translocation of C/EBPβ into the nucleus, which was achieved by using a system utilizing the fusion protein between C/EBPβ and the ligand-binding domain of the human estrogen receptor (C/EBPβ-ERT), demonstrated the rapid induction of endogenous Id2 expression. In reporter assays, transactivation of the Id2 promoter by C/EBPβ was observed and, among three potential C/EBPβ binding sites found in the 2.3 kb Id2 promoter region, the most proximal element was responsible for the transactivation. Electrophoretic mobility shift assay (EMSA) identified this element as a core sequence to which C/EBPβ binds. Chromatin immunoprecipitation (ChIP) furthermore confirmed the presence of C/EBPβ in the Id2 promoter region. Northern blotting showed that Id2 expression in C/EBPβ-deficient mammary glands was reduced at 10 days post coitus (d.p.c.), compared with that in wild-type mammary glands. Thus, our data demonstrate that Id2 is a direct target of C/EBPβ and provide insight into molecular mechanisms underlying mammary gland development during pregnancy. PMID:15809228

  10. An ID Network System to Prepare for Global Environmental/Health Concerns

    NASA Astrophysics Data System (ADS)

    Asano, Shoichiro; Yoneda, Susumu

    Climate change and/or pandemics are global life threatening concerns. For verifying and utilizing monitored data for solving to the Climate Change concerns, a network system based on device ID would be proposed. In this paper, we review the recent standardization initiatives in ITU-T, and propose an ID network that can be used to verify the solutions.

  11. 39. CALCINER CELL PLANS. TOGETHER WITH HAER ID33C37 ILLUSTRATES COMPLEXITY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    39. CALCINER CELL PLANS. TOGETHER WITH HAER ID-33-C-37 ILLUSTRATES COMPLEXITY OF PIPING. INEEL DRAWING NUMBER 200-0633-00-287-106445. FLUOR NUMBER 5775-CPP-633-P-50 - Idaho National Engineering Laboratory, Old Waste Calcining Facility, Scoville, Butte County, ID

  12. 40. CALCINER CELL SECTIONS. TOGETHER WITH HAER ID33C37 ILLUSTRATES COMPLEXITY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    40. CALCINER CELL SECTIONS. TOGETHER WITH HAER ID-33-C-37 ILLUSTRATES COMPLEXITY OF PIPING. INEEL DRAWING NUMBER 200-0633-00-287-106446. FLUOR NUMBER 5775-CPP-P-51. - Idaho National Engineering Laboratory, Old Waste Calcining Facility, Scoville, Butte County, ID

  13. Documenting the Undocumented: A Review of the United States' First Municipal ID Program

    ERIC Educational Resources Information Center

    Lagunes, Paul F.; Levin, Brian M.; Ditlmann, Ruth K.

    2012-01-01

    The City of New Haven, CT, sought to promote the assimilation of its immigrants by becoming the first American city to provide a government-backed ID, the Elm City Resident Card, to all residents. This article tests the policy's effectiveness in commerce. We find that Hispanics/Latinos are asked to present an ID more than Whites/Anglos, showing…

  14. ID Proteins Regulate Diverse Aspects of Cancer Progression and Provide Novel Therapeutic Opportunities

    PubMed Central

    Nair, Radhika; Teo, Wee Siang; Mittal, Vivek; Swarbrick, Alexander

    2014-01-01

    The inhibitor of differentiation (ID) proteins are helix-loop-helix transcriptional repressors with established roles in stem cell self-renewal, lineage commitment, and niche interactions. While deregulated expression of ID proteins in cancer was identified more than a decade ago, emerging evidence has revealed a central role for ID proteins in neoplastic progression of multiple tumor types that often mirrors their function in physiological stem and progenitor cells. ID proteins are required for the maintenance of cancer stem cells, self-renewal, and proliferation in a range of malignancies. Furthermore, ID proteins promote metastatic dissemination through their role in remodeling the tumor microenvironment and by promoting tumor-associated endothelial progenitor cell proliferation and mobilization. Here, we discuss the latest findings in this area and the clinical opportunities that they provide. PMID:24827908

  15. Ubiquitin-SUMO circuitry controls activated fanconi anemia ID complex dosage in response to DNA damage.

    PubMed

    Gibbs-Seymour, Ian; Oka, Yasuyoshi; Rajendra, Eeson; Weinert, Brian T; Passmore, Lori A; Patel, Ketan J; Olsen, Jesper V; Choudhary, Chunaram; Bekker-Jensen, Simon; Mailand, Niels

    2015-01-01

    We show that central components of the Fanconi anemia (FA) DNA repair pathway, the tumor suppressor proteins FANCI and FANCD2 (the ID complex), are SUMOylated in response to replication fork stalling. The ID complex is SUMOylated in a manner that depends on the ATR kinase, the FA ubiquitin ligase core complex, and the SUMO E3 ligases PIAS1/PIAS4 and is antagonized by the SUMO protease SENP6. SUMOylation of the ID complex drives substrate selectivity by triggering its polyubiquitylation by the SUMO-targeted ubiquitin ligase RNF4 to promote its removal from sites of DNA damage via the DVC1-p97 ubiquitin segregase complex. Deregulation of ID complex SUMOylation compromises cell survival following replication stress. Our results uncover a regulatory role for SUMOylation in the FA pathway, and we propose that ubiquitin-SUMO signaling circuitry is a mechanism that contributes to the balance of activated ID complex dosage at sites of DNA damage. PMID:25557546

  16. Six steps in quality intervention development (6SQuID).

    PubMed

    Wight, Daniel; Wimbush, Erica; Jepson, Ruth; Doi, Lawrence

    2016-05-01

    Improving the effectiveness of public health interventions relies as much on the attention paid to their design and feasibility as to their evaluation. Yet, compared to the vast literature on how to evaluate interventions, there is little to guide researchers or practitioners on how best to develop such interventions in practical, logical, evidence based ways to maximise likely effectiveness. Existing models for the development of public health interventions tend to have a strong social-psychological, individual behaviour change orientation and some take years to implement. This paper presents a pragmatic guide to six essential Steps for Quality Intervention Development (6SQuID). The focus is on public health interventions but the model should have wider applicability. Once a problem has been identified as needing intervention, the process of designing an intervention can be broken down into six crucial steps: (1) defining and understanding the problem and its causes; (2) identifying which causal or contextual factors are modifiable: which have the greatest scope for change and who would benefit most; (3) deciding on the mechanisms of change; (4) clarifying how these will be delivered; (5) testing and adapting the intervention; and (6) collecting sufficient evidence of effectiveness to proceed to a rigorous evaluation. If each of these steps is carefully addressed, better use will be made of scarce public resources by avoiding the costly evaluation, or implementation, of unpromising interventions. PMID:26573236

  17. Comparison of ID performance using real and synthetic imagery

    NASA Astrophysics Data System (ADS)

    Jacobs, Eddie L.; Edwards, Timothy C.; Miller, Brian; Hodgkin, Van A.

    2002-07-01

    Recent experiments performed at the U.S. Army Night Vision and Electronic Sensors Directorate (NVESD) provide significant insight into the validation of synthetic imagery for use in human perception experiments. This paper documents the procedures and results of target identification (ID) experiments using real and synthetic thermal imagery. Real imagery representing notional first generation and advanced scanning sensor systems was obtained. Parameters derived from the sensor data were used to generate synthetic imagery using the NVESD Paint the Night simulation. Both image sets were then used in a target identification experiment with trained human observers. Perception test results were analyzed and compared with metrics derived from the imagery. Several parameters missing from the original truth data were found to correlate with differences in the perception data. Synthetic data were regenerated using these additional parameters. A subsequent perception experiment confirmed the importance of these parameters, and a good match was obtained between real and synthetic imagery. While the techniques used in this series of experiments do not constitute a definitive method for validating synthetic imagery, they point to some important observations on validation. The main observation is that both target and local background characteristics must be sufficiently specified in the truth data in order to obtain good agreement between synthetic and real data. The paper concludes with suggestions as to the level of detail necessary for truth data when using synthetic imagery in perception experiments.

  18. A neural-visualization IDS for honeynet data.

    PubMed

    Herrero, Álvaro; Zurutuza, Urko; Corchado, Emilio

    2012-04-01

    Neural intelligent systems can provide a visualization of the network traffic for security staff, in order to reduce the widely known high false-positive rate associated with misuse-based Intrusion Detection Systems (IDSs). Unlike previous work, this study proposes an unsupervised neural models that generate an intuitive visualization of the captured traffic, rather than network statistics. These snapshots of network events are immensely useful for security personnel that monitor network behavior. The system is based on the use of different neural projection and unsupervised methods for the visual inspection of honeypot data, and may be seen as a complementary network security tool that sheds light on internal data structures through visual inspection of the traffic itself. Furthermore, it is intended to facilitate verification and assessment of Snort performance (a well-known and widely-used misuse-based IDS), through the visualization of attack patterns. Empirical verification and comparison of the proposed projection methods are performed in a real domain, where two different case studies are defined and analyzed. PMID:23627591

  19. Molecular characterization of lactobacilli isolated from fermented idli batter

    PubMed Central

    Agaliya, Perumal Jayaprabha; Jeevaratnam, Kadirvelu

    2013-01-01

    Lactic acid bacteria are non pathogenic organism widely distributed in nature typically involved in a large number of spontaneous food fermentation. The purpose of this study was to characterize the bacteriocinogenic lactobacilli from fermented idli batter which can find application in biopreservation and biomedicine. Eight most promising lactobacilli were chosen from twenty two isolates based on their spectrum of activity against other lactic acid bacteria and pathogens. The eight lactobacilli were characterized based on the various classical phenotypic tests, physiological tests and biochemical tests including various carbohydrate utilization profiles. All isolates were homo fermentative, catalase, and gelatin negative. Molecular characterization was performed by RAPD, 16S rRNA analysis, 16S ARDRA, and Multiplex PCR for species identification. RAPD was carried out using the primer R2 and M13. Five different clusters were obtained based on RAPD indicating strain level variation. 16S rRNA analysis showed 99 to 100% homology towards Lactobacillus plantarum. The restriction digestion pattern was similar for all the isolates with the restriction enzyme AluI. The subspecies were identified by performing Multiplex PCR using species specific primer. Among the five clusters, three clusters were clearly identified as Lactobacillus plantarum subsp. plantarum, Lactobacillus pentosus, and Lactobacillus plantarum subsp. argentoratensis. PMID:24688512

  20. Six steps in quality intervention development (6SQuID)

    PubMed Central

    Wight, Daniel; Wimbush, Erica; Jepson, Ruth; Doi, Lawrence

    2016-01-01

    Improving the effectiveness of public health interventions relies as much on the attention paid to their design and feasibility as to their evaluation. Yet, compared to the vast literature on how to evaluate interventions, there is little to guide researchers or practitioners on how best to develop such interventions in practical, logical, evidence based ways to maximise likely effectiveness. Existing models for the development of public health interventions tend to have a strong social-psychological, individual behaviour change orientation and some take years to implement. This paper presents a pragmatic guide to six essential Steps for Quality Intervention Development (6SQuID). The focus is on public health interventions but the model should have wider applicability. Once a problem has been identified as needing intervention, the process of designing an intervention can be broken down into six crucial steps: (1) defining and understanding the problem and its causes; (2) identifying which causal or contextual factors are modifiable: which have the greatest scope for change and who would benefit most; (3) deciding on the mechanisms of change; (4) clarifying how these will be delivered; (5) testing and adapting the intervention; and (6) collecting sufficient evidence of effectiveness to proceed to a rigorous evaluation. If each of these steps is carefully addressed, better use will be made of scarce public resources by avoiding the costly evaluation, or implementation, of unpromising interventions. PMID:26573236

  1. Pros and Cons of ID vs. 3D Modeling

    NASA Technical Reports Server (NTRS)

    Klimchuk, James A.

    2012-01-01

    Advances in computing capability have led to tremendous improvements in 3D modeling. Entire active regions are being simulated in what might be described as a first principles way, in which plasma heating is treated self consistently rather than through the specification of heating functions. There are limitations to this approach, however, as actual heating mechanisms on the Sun involve spatial scales orders of magnitude smaller than what these simulations can resolve. Other simulations begin to resolve these scales, but they only treat a tiny volume and do not include the all important coupling with larger scales or with other parts of the atmosphere, and so cannot be readily compared with observations. Finally, ID hydrodynamic models capture the field-aligned evolution of the plasma extremely well and are ideally suited for data comparison, but they treat the heating in a totally ad hoc manner. All of these approaches have important contributions to make, but we must be aware of their limitations. I will highlight some of the strengths. and weaknesses of each.

  2. Minimum wafer thickness by rotated ingot ID wafering. [Inner Diameter

    NASA Technical Reports Server (NTRS)

    Chen, C. P.; Leipold, M. H.

    1984-01-01

    The efficient utilization of materials is critical to certain device applications such as silicon for photovoltaics or diodes and gallium-gadolinium-garnet for memories. A variety of slicing techniques has been investigated to minimize wafer thickness and wafer kerf. This paper presents the results of analyses of ID wafering of rotated ingots based on predicted fracture behavior of the wafer as a result of forces during wafering and the properties of the device material. The analytical model indicated that the minimum wafer thickness is controlled by the depth of surface damage and the applied cantilever force. Both of these factors should be minimized. For silicon, a minimum thickness was found to be approximately 200 x 10 - 6th m for conventional sizes of rotated ingot wafering. Fractures through the thickness of the wafer rather than through the center supporting column were found to limit the minimum wafer thickness. The model suggested that the use of a vacuum chuck on the wafer surface to enhance cleavage fracture of the center supporting core and, with silicon, by using 111-line-type ingots could have potential for reducing minimum wafer thickness.

  3. Senior Administrators Should Have Administrative Contracts.

    ERIC Educational Resources Information Center

    Posner, Gary J.

    1987-01-01

    Recognizing that termination is viewed by the employee as the equivalent to capital punishment of a career, an administrative contract can reduce the emotional and financial entanglements that often result. Administrative contracts are described. (MLW)

  4. Identifier (ID) elements are not preferentially located to brain-specific genes: high ID element representation in other tissue-specific- and housekeeping genes of the rat.

    PubMed

    Goldman, Andrés; Capoano, Carlos A; González-López, Evangelina; Geisinger, Adriana

    2014-01-01

    BC1 is a short non-coding RNA from rodents, which is transcribed by RNA pol III. Its RNA is highly abundant in the brain, where it exerts a post-transcriptional regulatory role in dendrites. Upon transcription, retroposition and insertion, BC1 gives rise to a subclass of short interspersed repetitive sequences (SINEs) named identifier (ID) elements. IDs can become integrated inside non-coding regions of RNA pol II transcription units, and - although challenged by a couple of reports - their preferential location to brain-specific genes has been long proposed. Furthermore, an additional, cis-regulatory role in the control of brain-specific pol II-directed transcripts has been suggested for these sequences. In this work we used Northern blot and in silico analyses to examine IDs' location among pol II transcription units in different tissues, and in housekeeping genes. ID sequences appeared distributed in a similar fashion within tissue-specific hnRNA populations of the brain, testis and liver, and within housekeeping primary transcripts as well. Moreover, when the lengths of the unprocessed transcripts were considered, ID representation was higher in housekeeping ones. On the other hand, ID elements appeared similarly distributed among the different gene regions, with the obvious exclusion of those sequences where strict constraints for proper gene expression exist. Altogether, the widespread distribution of ID elements in all the analyzed genes - including housekeeping - and in all gene regions, suggests a random location, raising questions about the specific cis-regulatory role of those sequences. PMID:24125954

  5. Novice Administrators: Personality and Administrative Style Changes.

    ERIC Educational Resources Information Center

    Schmidt, Linda J.; Kosmoski, Georgia J.; Pollack, Dennis R.

    Since the advent of effective-schools research findings, educational administration experts have advocated a democratic and collegial leadership style for school administrators. This paper provides the findings of a study that examined 43 beginning administrators (25 females, 32 Caucasians, 9 African-Americans, 2 Hispanics) to determine what…

  6. Long-range optical character recognition for product ID

    NASA Astrophysics Data System (ADS)

    Banta, Larry E.; Pertl, Franz A.; Rosenberry-Friend, Kimberly A.

    1998-10-01

    An automated product tag reading system based on CCD cameras and computer image processing has been developed by West Virginia University and demonstrated at the Weirton Steel Corporation. The system reads both a 50-mil barcode and a string of six numbers on a four-inch by six-inch tag fastened to the end of a steel slab. Feedback from the image is used to point and zoom the camera, making the system effective at ranges up to thirty feet, and in bright sunlight-situations where handheld barcode scanners are ineffective. A video camera is mounted on a pan/tilt head and connected to a personal computer through a frame grabber board. The whole system is mounted on a slab hauler--a huge wheeled machine for carrying 100 tons of steel slabs at a time. The slab hauler backs into position and presses `start' on a touchscreen operator interface. A wide-angle image is grabbed, and the computer analyzes the images to find product ID tags in scene. The camera is then zoomed and pointed one-by-one at the tags for closeup images. Geometric warping is done on the closeup images to correct for viewing angle distortion, and both the barcode and the alphanumeric code are read by the software and reported to the inventory management system via radio modern. This paper discusses the neural network-based system for reading the characters on the tag. The camera pointing system and barcode reader are discussed in a companion paper.

  7. SpDamID: Marking DNA Bound by Protein Complexes Identifies Notch-Dimer Responsive Enhancers

    PubMed Central

    Hass, Matthew R.; Liow, Hien-haw; Chen, Xiaoting; Sharma, Ankur; Inoue, Yukiko U.; Inoue, Takayoshi; Reeb, Ashley; Martens, Andrew; Fulbright, Mary; Raju, Saravanan; Stevens, Michael; Boyle, Scott; Park, Joo-Seop; Weirauch, Matthew T.; Brent, Michael; Kopan, Raphael

    2015-01-01

    SUMMARY We developed Split DamID (SpDamID), a protein complementation version of DamID, to mark genomic DNA bound in vivo by interacting or juxtapositioned transcription factors. Inactive halves of DAM (DNA Adenine Methyltransferase) were fused to protein pairs to be queried Interaction or proximity enabled DAM reconstitution and methylation of adenine in GATC. Inducible SpDamID was used to analyze Notch-mediated transcriptional activation. We demonstrate that Notch complexes label RBP sites broadly across the genome, and show that a subset of these complexes that recruit MAML and p300 undergo changes in chromatin accessibility in response to Notch signaling. SpDamID differentiates between monomeric and dimeric binding thereby allowing for identification of half-site motifs used by Notch dimers. Motif enrichment of Notch enhancers coupled with SpDamID reveals co-targeting of regulatory sequences by Notch and Runx1. SpDamID represents a sensitive and powerful tool that enables dynamic analysis of combinatorial protein-DNA transactions at a genome-wide level. PMID:26257285

  8. SpDamID: Marking DNA Bound by Protein Complexes Identifies Notch-Dimer Responsive Enhancers.

    PubMed

    Hass, Matthew R; Liow, Hien-Haw; Chen, Xiaoting; Sharma, Ankur; Inoue, Yukiko U; Inoue, Takayoshi; Reeb, Ashley; Martens, Andrew; Fulbright, Mary; Raju, Saravanan; Stevens, Michael; Boyle, Scott; Park, Joo-Seop; Weirauch, Matthew T; Brent, Michael R; Kopan, Raphael

    2015-08-20

    We developed Split DamID (SpDamID), a protein complementation version of DamID, to mark genomic DNA bound in vivo by interacting or juxtapositioned transcription factors. Inactive halves of DAM (DNA adenine methyltransferase) were fused to protein pairs to be queried. Either direct interaction between proteins or proximity enabled DAM reconstitution and methylation of adenine in GATC. Inducible SpDamID was used to analyze Notch-mediated transcriptional activation. We demonstrate that Notch complexes label RBP sites broadly across the genome and show that a subset of these complexes that recruit MAML and p300 undergo changes in chromatin accessibility in response to Notch signaling. SpDamID differentiates between monomeric and dimeric binding, thereby allowing for identification of half-site motifs used by Notch dimers. Motif enrichment of Notch enhancers coupled with SpDamID reveals co-targeting of regulatory sequences by Notch and Runx1. SpDamID represents a sensitive and powerful tool that enables dynamic analysis of combinatorial protein-DNA transactions at a genome-wide level. PMID:26257285

  9. Mesenchymal high-grade glioma is maintained by the ID-RAP1 axis

    PubMed Central

    Niola, Francesco; Zhao, Xudong; Singh, Devendra; Sullivan, Ryan; Castano, Angelica; Verrico, Antonio; Zoppoli, Pietro; Friedmann-Morvinski, Dinorah; Sulman, Erik; Barrett, Lindy; Zhuang, Yuan; Verma, Inder; Benezra, Robert; Aldape, Ken; Iavarone, Antonio; Lasorella, Anna

    2012-01-01

    High-grade gliomas (HGGs) are incurable brain tumors that are characterized by the presence of glioma-initiating cells (GICs). GICs are essential to tumor aggressiveness and retain the capacity for self-renewal and multilineage differentiation as long as they reside in the perivascular niche. ID proteins are master regulators of stemness and anchorage to the extracellular niche microenvironment, suggesting that they may play a role in maintaining GICs. Here, we modeled the probable therapeutic impact of ID inactivation in HGG by selective ablation of Id in tumor cells and after tumor initiation in a new mouse model of human mesenchymal HGG. Deletion of 3 Id genes induced rapid release of GICs from the perivascular niche, followed by tumor regression. GIC displacement was mediated by derepression of Rap1gap and subsequent inhibition of RAP1, a master regulator of cell adhesion. We identified a signature module of 5 genes in the ID pathway, including RAP1GAP, which segregated 2 subgroups of glioma patients with markedly different clinical outcomes. The model-informed survival analysis together with genetic and functional studies establish that ID activity is required for the maintenance of mesenchymal HGG and suggest that pharmacological inactivation of ID proteins could serve as a therapeutic strategy. PMID:23241957

  10. Id-1 is a Key Transcriptional Regulator of Glioblastoma Aggressiveness and a Novel Therapeutic Target

    PubMed Central

    Soroceanu, Liliana; Murase, Ryuichi; Limbad, Chandani; Singer, Eric; Allison, Juanita; Adrados, Isabel; Kawamura, Rumi; Pakdel, Arash; Fukuyo, Yayoi; Nguyen, Daniel; Khan, Sabeena; Arauz, Robert; Yount, Garret L.; Moore, Dan H.; Desprez, Pierre-Yves; McAllister, Sean D.

    2012-01-01

    Glioblastoma (GBM) is the most common form of primary adult brain tumors. A majority of GBMs grow invasively into distant brain tissue, leading to tumor recurrence, which is ultimately incurable. It is, therefore, essential to discover master regulators that control GBM invasiveness and target them therapeutically. We demonstrate here that the transcriptional regulator Id-1 plays a critical role in modulating the invasiveness of GBM cell lines and primary GBM cells. Id-1 expression levels positively correlate with glioma cell invasiveness in culture and with histopathological grades in patient biopsies. Id-1 knockdown dramatically reduces GBM cell invasion that is accompanied by profound morphological changes and robust reduction in expression levels of “mesenchymal” markers, as well as inhibition of self-renewal potential and down-regulation of glioma stem cell markers. Importantly, genetic knockdown of Id-1 leads to a significant increase in survival in an orthotopic model of human GBM. Furthermore, we show that a non-toxic compound, cannabidiol, significantly down-regulates Id-1 gene expression and associated glioma cell invasiveness and self-renewal. Additionally, cannabidiol significantly inhibits the invasion of GBM cells through an organotypic brain slice and glioma progression in vivo. Our results suggest that Id-1 regulates multiple tumor-promoting pathways in GBM, and that drugs targeting Id-1 represent a novel and promising strategy for improving the therapy and outcome of GBM patients. PMID:23243024

  11. The ID23-2 structural biology microfocus beamline at the ESRF

    PubMed Central

    Flot, David; Mairs, Trevor; Giraud, Thierry; Guijarro, Matias; Lesourd, Marc; Rey, Vicente; van Brussel, Denis; Morawe, Christian; Borel, Christine; Hignette, Olivier; Chavanne, Joel; Nurizzo, Didier; McSweeney, Sean; Mitchell, Edward

    2010-01-01

    The first phase of the ESRF beamline ID23 to be constructed was ID23-1, a tunable MAD-capable beamline which opened to users in early 2004. The second phase of the beamline to be constructed is ID23-2, a monochromatic microfocus beamline dedicated to macromolecular crystallography experiments. Beamline ID23-2 makes use of well characterized optical elements: a single-bounce silicon (111) monochromator and two mirrors in Kirkpatrick–Baez geometry to focus the X-ray beam. A major design goal of the ID23-2 beamline is to provide a reliable, easy-to-use and routine microfocus beam. ID23-2 started operation in November 2005, as the first beamline dedicated to microfocus macromolecular crystallography. The beamline has taken the standard automated ESRF macromolecular crystallography environment (both hardware and software), allowing users of ID23-2 to be rapidly familiar with the microfocus environment. This paper describes the beamline design, the special considerations taken into account given the microfocus beam, and summarizes the results of the first years of the beamline operation. PMID:20029119

  12. Bio-fortification and shelf-life extension of idli batter using curry leaves (Murraya koenigii).

    PubMed

    Chelliah, R; Ramakrishnan, S R; Premkumar, D; Antony, U

    2016-06-01

    Among several traditional foods of India, idli is one of the most popular and commonly consumed steamed products. A new method of adding Murraya koenigii (curry leaves) to idli batter as a vehicle for fortification and extension of shelf-life has been developed. Dried curry leaves powder was incorporated with other ingredients like rice and dehusked black gram in different proportions to optimize the most palatable formulation. Rate of fermentation and microbial changes in the batter; nutritional qualities, texture and sensory properties of the prepared product were assessed. Incorporation of curry leaves powder (5 %) in idli batter increased the shelf-life and also increased the flavour, texture and appearance of the idli. The calcium content of the prepared idli was 10 times more than that of the control idli, while dietary fiber content increased by 18.6 %. Anti-microbial activity of the curry leaves in idli batter extended the shelf-life from 2 to 5 days when stored at 30 °C. PMID:27478242

  13. Id1 expression promotes T regulatory cell differentiation by facilitating TCR costimulation

    PubMed Central

    Jin, Rong; Tang, Hui; Liu, Yuan-Feng; Ge, Qing; Sun, Xiao-Hong; Zhang, Yu

    2014-01-01

    T Regulatory cells (Treg) play crucial roles in the regulation of cellular immunity. The development of Treg cells depends on signals from T cell receptors (TCR) and IL-2 receptors and is influenced by a variety of transcription factors. The basic helix-loop-helix (bHLH) proteins are known to influence TCR signaling thresholds. Whether this property impacts Treg differentiation is not understood. Here, we interrogated the role of bHLH proteins in the production of Treg cells using the CD4 promoter-driven Id1 transgene. We found that Treg cells continued to accumulate as Id1 transgenic mice aged, resulting in a significant increase in Treg cell counts in the thymus as well as in the periphery compared to wild type controls. Data from mixed-bone marrow assays suggest that Id1 acts intrinsically on developing Treg cells. We made a connection between Id1 expression and CD28 co-stimulatory signaling because Id1 transgene expression facilitated the formation of Treg precursors in CD28−/− mice and the in vitro differentiation of Treg cells on thymic dendritic cells despite the blockade of costimulation by anti-CD80/CD86. Id1 expression also allowed in vitro Treg differentiation without anti-CD28 co-stimulation, which was at least in part due to enhanced production of IL-2. Notably, with full strength of co-stimulatory signals, however, Id1 expression caused modest but significant suppression of Treg induction. Finally, we demonstrate that Id1 transgenic mice were less susceptible to the induction of experimental autoimmune encephalomyelitis (EAE), thus illustrating the impact of Id1-mediated augmentation of Treg cell levels on cellular immunity. PMID:24920844

  14. ADM. Aerial view of administration area. Camera facing westerly. From ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    ADM. Aerial view of administration area. Camera facing westerly. From left to right in foregound: Substation (TAN-605), Warehouse (TAN-628), Gate House (TAN-601), Administration Building (TAN-602). Left to right middle ground: Service Building (TAN-603), Warehouse (later known as Maintenance Shop or Craft Shop, TAN-604), Water Well Pump Houses, Fuel Tanks and Fuel Pump Houses, and Water Storage Tanks. Change House (TAN-606) on near side of berm. Large building beyond berm is A&M. Building, TAN-607. Railroad tracks beyond lead from (unseen) turntable to the IET. Date: June 6, 1955. INEEL negative no. 13201 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID

  15. Id-1 gene and gene products as therapeutic targets for treatment of breast cancer and other types of carcinoma

    SciTech Connect

    Desprez, Pierre-Yves; Campisi, Judith

    2014-08-19

    A method for treatment of breast cancer and other types of cancer. The method comprises targeting and modulating Id-1 gene expression, if any, for the Id-1 gene, or gene products in breast or other epithelial cancers in a patient by delivering products that modulate Id-1 gene expression. When expressed, Id-1 gene is a prognostic indicator that cancer cells are invasive and metastatic.

  16. The metalloid arsenite induces nuclear export of Id3 possibly via binding to the N-terminal cysteine residues

    SciTech Connect

    Kurooka, Hisanori; Sugai, Manabu; Mori, Kentaro; Yokota, Yoshifumi

    2013-04-19

    Highlights: •Sodium arsenite induces cytoplasmic accumulation of Id3. •Arsenite binds to closely spaced N-terminal cysteine residues of Id3. •N-terminal cysteines are essential for arsenite-induced nuclear export of Id3. •Nuclear export of Id3 counteracts its transcriptional repression activity. -- Abstract: Ids are versatile transcriptional repressors that regulate cell proliferation and differentiation, and appropriate subcellular localization of the Id proteins is important for their functions. We previously identified distinct functional nuclear export signals (NESs) in Id1 and Id2, but no active NES has been reported in Id3. In this study, we found that treatment with the stress-inducing metalloid arsenite led to the accumulation of GFP-tagged Id3 in the cytoplasm. Cytoplasmic accumulation was impaired by a mutation in the Id3 NES-like sequence resembling the Id1 NES, located at the end of the HLH domain. It was also blocked by co-treatment with the CRM1-specific nuclear export inhibitor leptomycin B (LMB), but not with the inhibitors for mitogen-activated protein kinases (MAPKs). Importantly, we showed that the closely spaced N-terminal cysteine residues of Id3 interacted with the arsenic derivative phenylarsine oxide (PAO) and were essential for the arsenite-induced cytoplasmic accumulation, suggesting that arsenite induces the CRM1-dependent nuclear export of Id3 via binding to the N-terminal cysteines. Finally, we demonstrated that Id3 significantly repressed arsenite-stimulated transcription of the immediate-early gene Egr-1 and that this repression activity was inversely correlated with the arsenite-induced nuclear export. Our results imply that Id3 may be involved in the biological action of arsenite.

  17. Improvement and analysis of ID3 algorithm in decision-making tree

    NASA Astrophysics Data System (ADS)

    Xie, Xiao-Lan; Long, Zhen; Liao, Wen-Qi

    2015-12-01

    For the cooperative system under development, it needs to use the spatial analysis and relative technology concerning data mining in order to carry out the detection of the subject conflict and redundancy, while the ID3 algorithm is an important data mining. Due to the traditional ID3 algorithm in the decision-making tree towards the log part is rather complicated, this paper obtained a new computational formula of information gain through the optimization of algorithm of the log part. During the experiment contrast and theoretical analysis, it is found that IID3 (Improved ID3 Algorithm) algorithm owns higher calculation efficiency and accuracy and thus worth popularizing.

  18. Final evaluation & test report for the standard waste box (docket 01-53-7A) type A packaging

    SciTech Connect

    KELLY, D L

    2001-10-15

    This report documents the U.S. Department of Transportation Specification 7A Type A compliance test and evaluation results of the Standard Waste Box. Testing and evaluation activities documented herein are on behalf of the U.S. Department of Energy-Headquarters, Office of Safety, Health and Security (EM-5), Germantown, Maryland. Duratek Federal Services, Inc., Northwest Operations performed an evaluation of the changes as documented herein under Docket 01-53-7A.

  19. 6 CFR 37.5 - Validity periods and deadlines for REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 6 Domestic Security 1 2011-01-01 2011-01-01 false Validity periods and deadlines for REAL ID... SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS General § 37.5 Validity periods and deadlines for REAL ID driver's licenses and identification cards. (a)...

  20. Wnt/{beta}-catenin signaling changes C2C12 myoblast proliferation and differentiation by inducing Id3 expression

    SciTech Connect

    Zhang, Long; Shi, Songting; Zhang, Juan; Zhou, Fangfang; Dijke, Peter ten

    2012-03-02

    Highlights: Black-Right-Pointing-Pointer Expression of Id3 but not Id1 is induced by Wnt3a stimulation in C2C12 cells. Black-Right-Pointing-Pointer Wnt3a induces Id3 expression via canonical Wnt/{beta}-catenin pathway. Black-Right-Pointing-Pointer Wnt3a-induced Id3 expression does not depend on BMP signaling activation. Black-Right-Pointing-Pointer Induction of Id3 expression is critical determinant in Wnt3a-induced cell proliferation and differentiation. -- Abstract: Canonical Wnt signaling plays important roles in regulating cell proliferation and differentiation. In this study, we report that inhibitor of differentiation (Id)3 is a Wnt-inducible gene in mouse C2C12 myoblasts. Wnt3a induced Id3 expression in a {beta}-catenin-dependent manner. Bone morphogenetic protein (BMP) also potently induced Id3 expression. However, Wnt-induced Id3 expression occurred independent of the BMP/Smad pathway. Functional studies showed that Id3 depletion in C2C12 cells impaired Wnt3a-induced cell proliferation and alkaline phosphatase activity, an early marker of osteoblast cells. Id3 depletion elevated myogenin induction during myogenic differentiation and partially impaired Wnt3a suppressed myogenin expression in C2C12 cells. These results suggest that Id3 is an important Wnt/{beta}-catenin induced gene in myoblast cell fate determination.

  1. Family Impact in Intellectual Disability, Severe Mental Health Disorders and Mental Health Disorders in ID. A Comparison

    ERIC Educational Resources Information Center

    Martorell, Almudena; Gutierrez-Recacha, Pedro; Irazabal, Marcia; Marsa, Ferran; Garcia, Mercedes

    2011-01-01

    Family impact (or family burden) is a concept born in the field of mental health that has successfully been exported to the ambit of intellectual disability (ID). However, differences in family impact associated with severe mental health disorders (schizophrenia), to ID or to mental health problems in ID should be expected. Seventy-two adults with…

  2. 6 CFR 37.5 - Validity periods and deadlines for REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 6 Domestic Security 1 2010-01-01 2010-01-01 false Validity periods and deadlines for REAL ID driver's licenses and identification cards. 37.5 Section 37.5 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS General § 37.5 Validity periods and deadlines for REAL ID...

  3. 6 CFR 37.5 - Validity periods and deadlines for REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 6 Domestic Security 1 2012-01-01 2012-01-01 false Validity periods and deadlines for REAL ID... SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS General § 37.5 Validity periods and deadlines for REAL ID driver's licenses and identification cards. (a)...

  4. 6 CFR 37.5 - Validity periods and deadlines for REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 6 Domestic Security 1 2013-01-01 2013-01-01 false Validity periods and deadlines for REAL ID... SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS General § 37.5 Validity periods and deadlines for REAL ID driver's licenses and identification cards. (a)...

  5. 6 CFR 37.5 - Validity periods and deadlines for REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 6 Domestic Security 1 2014-01-01 2014-01-01 false Validity periods and deadlines for REAL ID... SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS General § 37.5 Validity periods and deadlines for REAL ID driver's licenses and identification cards. (a)...

  6. 78 FR 48868 - Proposed Cercla Administrative Cost Recovery Settlement; MassDOT, MassDOT Route 1 Right-of-Way...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-12

    ... AGENCY Proposed Cercla Administrative Cost Recovery Settlement; MassDOT, MassDOT Route 1 Right-of-Way...), concerning the MassDOT Route 1 Right-of-Way Site in Chelsea, Massachusetts with the following Settling Party... should refer to: In re: MassDOT Route 1 Right-of- Way Site, U.S. EPA Docket No.01-2013-0031. FOR...

  7. ADM. Administration Building (TAN602). Elevations, sections, details. Shows areas that ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    ADM. Administration Building (TAN-602). Elevations, sections, details. Shows areas that were soon remodeled or added onto. Ralph M. Parsons 902-2-ANP-602-A 32 Date: August 1955. Approved by INEEL Classification Office for public release. INEEL index code no. 033-0602-00-693-106711 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID

  8. 78 FR 56860 - Honey From the People's Republic of China: Final Results of Antidumping Duty Administrative...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-16

    ... and Partial Rescission of Antidumping Duty Administrative Review; 2011-2012, 78 FR 38941 (June 28... Antidumping Proceedings: Assessment of Antidumping Duties, 76 FR 65694 (October 24, 2011). \\6\\ See id. Cash... merchandise under the order is dispositive. Also included in the scope are blends of honey and rice...

  9. 132. ARAII Administration building (ARA613) elevations of north, south, east, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    132. ARA-II Administration building (ARA-613) elevations of north, south, east, and west sides. F. C. Torkelson Company 842-area/SL-1-613-A-2. Date: October 1959. Ineel index no. 070-0613-00-851-150054. - Idaho National Engineering Laboratory, Army Reactors Experimental Area, Scoville, Butte County, ID

  10. 129. ARAII Administrative and technical support building (ARA606) sections showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    129. ARA-II Administrative and technical support building (ARA-606) sections showing roof and wall details and longitudinal section. C.A. Sundberg and Associates 866-area/ALPR-606-A-5. Date: May 1958. Ineel index code no. 070-0606-00-822-102828. - Idaho National Engineering Laboratory, Army Reactors Experimental Area, Scoville, Butte County, ID

  11. 127. ARAII Administrative and technical support building (ARA606) ground floor ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    127. ARA-II Administrative and technical support building (ARA-606) ground floor plan. Indicates use of rooms for classrooms, offices, and lunch room. C.A. Sundberg and Associates 866-area-ALPR-606-A-2. Date: June 1958. Ineel index code no. 070-0606-00-822-102825. - Idaho National Engineering Laboratory, Army Reactors Experimental Area, Scoville, Butte County, ID

  12. 128. ARAII Administrative and technical support building (ARA606) elevations for ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    128. ARA-II Administrative and technical support building (ARA-606) elevations for northwest, southwest, northeast, and southeast sides. C.A. Sundberg and Associates 866-area/ALPR-606-A-3. Date: May 1958. Ineel index code no. 070-0606-00-822-102826. - Idaho National Engineering Laboratory, Army Reactors Experimental Area, Scoville, Butte County, ID

  13. 17 CFR 274.402 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... its Password. Editorial Note: For Federal Register citations affecting Form ID, see the List of CFR... SECURITIES AND EXCHANGE COMMISSION (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF...

  14. Id2 IS REQUIRED FOR SPECIFICATION OF DOPAMINERGIC NEURONS DURING ADULT OLFACTORY NEUROGENESIS

    PubMed Central

    Havrda, Matthew C.; Harris, Brent T.; Mantani, Akio; Ward, Nora M.; Paolella, Brenton R.; Cuzon, Verginia C.; Yeh, Hermes H.; Israel, Mark A.

    2009-01-01

    Understanding the biology of adult neural stem cells has important implications for nervous system development and may contribute to our understanding of neurodegenerative disorders and their treatment. We have characterized the process of olfactory neurogenesis in adult mice lacking Inhibitor of DNA Binding 2 (Id2). We found a diminished olfactory bulb containing reduced numbers of granular and periglomerular neurons with a distinct paucity of dopaminergic periglomerular neurons. While no deficiency of the stem cell compartment was detectable, migrating neuroblasts in Id2−/− mutant mice prematurely undergo astroglial differentiation within a disorganized rostral migratory stream. Further, when evaluated in vitro loss of Id2 results in decreased proliferation of neural progenitors and decreased expression of the Hes1 and Mash1 transcription factors, known mediators of neuronal differentiation. These data support a novel role for sustained Id2 expression in migrating neural progenitors mediating olfactory dopaminergic neuronal differentiation in adult animals. PMID:19109490

  15. 78 FR 68467 - Notice of Public Meetings, Twin Falls District Resource Advisory Council, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-14

    ... Resource Advisory Council (RAC) and subcommittee for the Greater sage-grouse will meet as indicated below... sage-grouse will meet at the Twin Falls District BLM Office, 2536 Kimberly Road, Twin Falls, ID...

  16. 75 FR 38767 - Intermountain Region, Boise, Payette, and Sawtooth National Forests; ID; Amendment to the 2003...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-06

    ... Forest Service Intermountain Region, Boise, Payette, and Sawtooth National Forests; ID; Amendment to the 2003 Land and Resource Management Plans: Wildlife Conservation Strategy (Forested Biological Community) AGENCY: Forest Service, USDA. ACTION: Third correction of notice of intent (NOI) to prepare...

  17. Organization/Administration.

    ERIC Educational Resources Information Center

    Chaffee, Ellen Earle

    Patterns that emerged from reviewing 26 syllabi for courses on organization and administration in higher education are discussed, and six sample syllabi are presented. The syllabi focused more on organization than administration. Of the 26 syllabi, 19 dealt with organization and administration generally; 5 with administration in a specific…

  18. GeneInfoMiner--a web server for exploring biomedical literature using batch sequence ID.

    PubMed

    Xuan, Weijian; Watson, Stanley J; Meng, Fan

    2005-08-15

    GeneInfoMiner is a web-based system for searching Medline abstracts using sequence ID lists such as GenBank accession numbers derived from high-throughput experiments. It will map query results to MeSH topics to facilitate the exploration of the biological significance of the sequence ID lists. GeneInfoMiner is based on a custom gene and protein name identification engine that can map gene and protein names to important molecular biology databases. PMID:15994195

  19. ETR BUILDING, TRA642, INTERIOR. BASEMENT. CUBICLE SHOWN IN ID33G101, ANOTHER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    ETR BUILDING, TRA-642, INTERIOR. BASEMENT. CUBICLE SHOWN IN ID-33-G-101, ANOTHER VIEW. PERSONNEL DOORWAY INTO CHAMBER IDENTIFIES SODIUM HAZARD AND POSSIBILITY OF INERT GAS. LIQUID SODIUM COOLANT WAS USED IN A SPECIAL ETR LOOP ADAPTED FOR IT IN 1972. INL NEGATIVE NO. HD24-3-2. Mike Crane, Photographer, 11/2000 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  20. ID box: a multisource attribute data fusion function for target identification

    NASA Astrophysics Data System (ADS)

    Simard, Marc-Alain; Valin, Pierre; Lesage, Frederic

    2000-04-01

    The R&D group at Lockheed-Martin Canada has developed a target identifier function called ID Box. This computer program performs five main functions: first it transforms the sensor attribute input into a few contact ID declarations, second, it evaluates the association score between the contact declarations and the ID propositions of a current target track, third it performs attribute contact to track fusion using a modification of the Dempster-Shafer evidential theory, fourth the ID Box, using a platform library, produces a translator that unifies the information within track identity and the attribute input, and fifth, it manages the distribution of results to a system human computer interface. Our exhaustive platform library enables the ID Box to fuse attribute data from almost all kinds of sensor or information sources that may be found on large warships or patrol aircraft. These attributes are the radar cross section and the moving parts from surveillance radars, allegiance from interrogator systems, emitter composition from electronics support measure systems, spoken language from communication intercept systems, acoustical signature from sonar systems, propulsion types from IR detectors, dimensional data from imaging systems and other classification attributes from various systems or operators including dynamical parameters from positional trackers. This paper presents and describes the ID Box.

  1. Gall-ID: tools for genotyping gall-causing phytopathogenic bacteria

    PubMed Central

    Tabima, Javier F.; Grunwald, Niklaus J.

    2016-01-01

    Understanding the population structure and genetic diversity of plant pathogens, as well as the effect of agricultural practices on pathogen evolution, is important for disease management. Developments in molecular methods have contributed to increase the resolution for accurate pathogen identification, but those based on analysis of DNA sequences can be less straightforward to use. To address this, we developed Gall-ID, a web-based platform that uses DNA sequence information from 16S rDNA, multilocus sequence analysis and whole genome sequences to group disease-associated bacteria to their taxonomic units. Gall-ID was developed with a particular focus on gall-forming bacteria belonging to Agrobacterium, Pseudomonas savastanoi, Pantoea agglomerans, and Rhodococcus. Members of these groups of bacteria cause growth deformation of plants, and some are capable of infecting many species of field, orchard, and nursery crops. Gall-ID also enables the use of high-throughput sequencing reads to search for evidence for homologs of characterized virulence genes, and provides downloadable software pipelines for automating multilocus sequence analysis, analyzing genome sequences for average nucleotide identity, and constructing core genome phylogenies. Lastly, additional databases were included in Gall-ID to help determine the identity of other plant pathogenic bacteria that may be in microbial communities associated with galls or causative agents in other diseased tissues of plants. The URL for Gall-ID is http://gall-id.cgrb.oregonstate.edu/. PMID:27547538

  2. Gall-ID: tools for genotyping gall-causing phytopathogenic bacteria.

    PubMed

    Davis Ii, Edward W; Weisberg, Alexandra J; Tabima, Javier F; Grunwald, Niklaus J; Chang, Jeff H

    2016-01-01

    Understanding the population structure and genetic diversity of plant pathogens, as well as the effect of agricultural practices on pathogen evolution, is important for disease management. Developments in molecular methods have contributed to increase the resolution for accurate pathogen identification, but those based on analysis of DNA sequences can be less straightforward to use. To address this, we developed Gall-ID, a web-based platform that uses DNA sequence information from 16S rDNA, multilocus sequence analysis and whole genome sequences to group disease-associated bacteria to their taxonomic units. Gall-ID was developed with a particular focus on gall-forming bacteria belonging to Agrobacterium, Pseudomonas savastanoi, Pantoea agglomerans, and Rhodococcus. Members of these groups of bacteria cause growth deformation of plants, and some are capable of infecting many species of field, orchard, and nursery crops. Gall-ID also enables the use of high-throughput sequencing reads to search for evidence for homologs of characterized virulence genes, and provides downloadable software pipelines for automating multilocus sequence analysis, analyzing genome sequences for average nucleotide identity, and constructing core genome phylogenies. Lastly, additional databases were included in Gall-ID to help determine the identity of other plant pathogenic bacteria that may be in microbial communities associated with galls or causative agents in other diseased tissues of plants. The URL for Gall-ID is http://gall-id.cgrb.oregonstate.edu/. PMID:27547538

  3. A role for Id in the regulation of TGF-beta-induced epithelial-mesenchymal transdifferentiation.

    PubMed

    Kondo, M; Cubillo, E; Tobiume, K; Shirakihara, T; Fukuda, N; Suzuki, H; Shimizu, K; Takehara, K; Cano, A; Saitoh, M; Miyazono, K

    2004-10-01

    Epithelial-mesenchymal transdifferentiation (EMT) is a critical morphogenic event that occurs during embryonic development and during the progression of various epithelial tumors. EMT can be induced by transforming growth factor (TGF)-beta in mouse NMuMG mammary epithelial cells. Here, we demonstrate a central role of helix-loop-helix factors, E2A and inhibitor of differentiation (Id) proteins, in TGF-beta-induced EMT. Epithelial cells ectopically expressing E2A adopt a fibroblastic phenotype and acquire migratory/invasive properties, concomitant with the suppression of E-cadherin expression. Id proteins interacted with E2A proteins and antagonized E2A-dependent suppression of the E-cadherin promoter. Levels of Id proteins were dramatically decreased by TGF-beta. Moreover, NMuMG cells overexpressed Id2 showed partial resistance to TGF-beta-induced EMT. Id proteins thus inhibit the action of E2A proteins on the expression of E-cadherin, but after TGF-beta stimulation, E2A proteins are present in molar excess of the Id proteins, thus over-riding their inhibitory function and leading to EMT. PMID:15181457

  4. The DNA-binding inhibitor Id3 regulates IL-9 production in CD4(+) T cells.

    PubMed

    Nakatsukasa, Hiroko; Zhang, Dunfang; Maruyama, Takashi; Chen, Hua; Cui, Kairong; Ishikawa, Masaki; Deng, Lisa; Zanvit, Peter; Tu, Eric; Jin, Wenwen; Abbatiello, Brittany; Goldberg, Nathan; Chen, Qianming; Sun, Lingyun; Zhao, Keji; Chen, WanJun

    2015-10-01

    The molecular mechanisms by which signaling via transforming growth factor-β (TGF-β) and interleukin 4 (IL-4) control the differentiation of CD4(+) IL-9-producing helper T cells (TH9 cells) remain incompletely understood. We found here that the DNA-binding inhibitor Id3 regulated TH9 differentiation, as deletion of Id3 increased IL-9 production from CD4(+) T cells. Mechanistically, TGF-β1 and IL-4 downregulated Id3 expression, and this process required the kinase TAK1. A reduction in Id3 expression enhanced binding of the transcription factors E2A and GATA-3 to the Il9 promoter region, which promoted Il9 transcription. Notably, Id3-mediated control of TH9 differentiation regulated anti-tumor immunity in an experimental melanoma-bearing model in vivo and also in human CD4(+) T cells in vitro. Thus, our study reveals a previously unrecognized TAK1-Id3-E2A-GATA-3 pathway that regulates TH9 differentiation. PMID:26322481

  5. [ID and Bio Vue systems--comparison of two systems for the typing of erythrocyte antibodies].

    PubMed

    Eichler, H; Klinge, U; Kretschmer, V

    1994-01-01

    The gel centrifugation test ID Microtyping was compared with the column agglutination test BioVue for antibody (AB) screening by testing serum samples of 3,000 patients under controlled conditions: enzyme test (ET; Bromelin) and LISS indirect antiglobulin test (IAT), both incubated for 15 min at 37 degrees C. From a total of 64 ABs, more relevant or possibly relevant ABs were detected by ID than by the BioVue system (63 vs. 56). ABs only detected in ID were: anti-D 3, -Jk(a), E 1, -Le(b)1, -e 1, -C 1, -E,S 1. One anti-Le(b) was only detected by BioVue. The calculated sensitivity was 98.4% for ID and 87.5% for BioVue. Furthermore, this study showed a lower frequency of unspecific reactions or reactions due to irrelevant cold ABs in the ID system (ID: 2.3%, BioVue: 4.0%, specificity 97.7 vs. 96.2%). PMID:9480072

  6. Differential role of Id1 in MLL-AF9-driven leukemia based on cell of origin.

    PubMed

    Man, Na; Sun, Xiao-Jian; Tan, Yurong; García-Cao, Marta; Liu, Fan; Cheng, Guoyan; Hatlen, Megan; Xu, Haiming; Shah, Ronit; Chastain, Nolan; Liu, Na; Huang, Gang; Zhou, Yuan; Sheng, Mengyao; Song, Junhong; Yang, Feng-Chun; Benezra, Robert; Nimer, Stephen D; Wang, Lan

    2016-05-12

    Inhibitor of DNA binding 1 (Id1) functions as an E protein inhibitor, and overexpression of Id1 is seen in acute myeloid leukemia (AML) patients. To define the effects of Id1 on leukemogenesis, we expressed MLL-AF9 in fetal liver (FL) cells or bone marrow (BM) cells isolated from wild-type, Id1(-/-), p21(-/-), or Id1(-/-)p21(-/-) mice, and transplanted them into syngeneic recipient mice. We found that although mice receiving MLL-AF9-transduced FL or BM cells develop AML, loss of Id1 significantly prolonged the median survival of mice receiving FL cells but accelerated leukemogenesis in recipients of BM cells. Deletion of Cdkn1a (p21), an Id1 target gene, can rescue the effect of Id1 loss in both models, suggesting that Cdkn1a is a critical target of Id1 in leukemogenesis. It has been suggested that the FL transplant model mimics human fetal-origin (infant) MLL fusion protein (FP)-driven leukemia, whereas the BM transplantation model resembles postnatal MLL leukemia; in fact, the analysis of clinical samples from patients with MLL-FP(+) leukemia showed that Id1 expression is elevated in the former and reduced in the latter type of MLL-FP(+) AML. Our findings suggest that Id1 could be a potential therapeutic target for infant MLL-AF9-driven leukemia. PMID:26944543

  7. Targeting ID2 expression triggers a more differentiated phenotype and reduces aggressiveness in human salivary gland cancer cells.

    PubMed

    Sumida, Tomoki; Ishikawa, Akiko; Nakano, Hiroyuki; Yamada, Tomohiro; Mori, Yoshihide; Desprez, Pierre-Yves

    2016-08-01

    Inhibitors of DNA-binding (ID) proteins are negative regulators of basic helix-loop-helix transcription factors and generally stimulate cell proliferation and inhibit differentiation. We previously determined that ID1 was highly expressed in aggressive salivary gland cancer (SGC) cells in culture. Here, we show that ID2 is also expressed in aggressive SGC cells. ID2 knockdown triggers important changes in cell behavior, that is, it significantly reduces the expression of N-cadherin, vimentin and Snail, induces E-cadherin expression and leads to a more differentiated phenotype exemplified by changes in cell shape. Moreover, ID2 knockdown almost completely suppresses invasion and the expression of matrix metalloproteinase 9. In conclusion, ID2 expression maintains an aggressive phenotype in SGC cells, and ID2 repression triggers a reduction in cell aggressiveness. ID2 therefore represents a potential therapeutic target during SGC progression. ID proteins are negative regulators of basic helix-loop-helix transcription factors and generally stimulate cell proliferation and inhibit differentiation. ID2 knockdown triggers important changes in cell behavior, that is, it significantly reduces the expression of N-cadherin, vimentin and Snail, induces E-cadherin expression and leads to a more differentiated phenotype exemplified by changes in cell shape. ID2 therefore represents a potential therapeutic target during SGC progression. PMID:27364596

  8. miR-342 Regulates BRCA1 Expression through Modulation of ID4 in Breast Cancer

    PubMed Central

    Crippa, Elisabetta; Lusa, Lara; De Cecco, Loris; Marchesi, Edoardo; Calin, George Adrian; Radice, Paolo; Manoukian, Siranoush; Peissel, Bernard; Daidone, Maria Grazia

    2014-01-01

    A miRNAs profiling on a group of familial and sporadic breast cancers showed that miRNA-342 was significantly associated with estrogen receptor (ER) levels. To investigate at functional level the role of miR-342 in the pathogenesis of breast cancer, we focused our attention on its “in silico” predicted putative target gene ID4, a transcription factor of the helix-loop-helix protein family whose expression is inversely correlated with that of ER. ID4 is expressed in breast cancer and can negatively regulate BRCA1 expression. Our results showed an inverse correlation between ID4 and miR-342 as well as between ID4 and BRCA1 expression. We functionally validated the interaction between ID4 and miR-342 in a reporter Luciferase system. Based on these findings, we hypothesized that regulation of ID4 mediated by miR-342 could be involved in the pathogenesis of breast cancer by downregulating BRCA1 expression. We functionally demonstrated the interactions between miR-342, ID4 and BRCA1 in a model provided by ER-negative MDA-MB-231 breast cancer cell line that presented high levels of ID4. Overexpression of miR-342 in these cells reduced ID4 and increased BRCA1 expression, supporting a possible role of this mechanism in breast cancer. In the ER-positive MCF7 and in the BRCA1-mutant HCC1937 cell lines miR-342 over-expression only reduced ID4. In the cohort of patients we studied, a correlation between miR-342 and BRCA1 expression was found in the ER-negative cases. As ER-negative cases were mainly BRCA1-mutant, we speculate that the mechanism we demonstrated could be involved in the decreased expression of BRCA1 frequently observed in non BRCA1-mutant breast cancers and could be implicated as a causal factor in part of the familial cases grouped in the heterogeneous class of non BRCA1 or BRCA2-mutant cases (BRCAx). To validate this hypothesis, the study should be extended to a larger cohort of ER-negative cases, including those belonging to the BRCAx class. PMID:24475217

  9. Dithranol downregulates expression of Id1 mRNA in human keratinocytes in vitro.

    PubMed

    Ronpirin, C; Tencomnao, T

    2012-01-01

    The precise causes of psoriasis, a chronic skin disorder characterized by hyperproliferation of keratinocytes and incomplete keratinization, are unclear. It is known that expression of helix-loop-helix transcription factor Id1, which functions as an inhibitor of differentiation, is upregulated in psoriatic skin. We investigated the effect of the antipsoriatic drug dithranol on mRNA and protein expression levels of Id1 in the HaCaT keratinocyte cell line. Cultured HaCaT cells were treated with 0-0.5 μg/mL dithranol for 30 min. After 2 and 4 h, total cellular RNA and total proteins were isolated from HaCaT cells, and quantitative real-time reverse transcriptase (RT-PCR) and Western blot were used to determine the mRNA and protein levels of Id1, respectively. Changes in normalized Id1 mRNA levels were observed only after 4 h of dithranol treatment. There was reduced expression of Id1 mRNA transcripts in the HaCaT cells treated with 0.1 μg/mL dithranol, but the reduction was not significant. The expression of Id1 mRNA was significantly downregulated (almost 50%) when 0.25 or 0.5 μg/mL dithranol was applied to the HaCaT cells. However, the normalized Id1 protein levels were not significantly affected. The molecular mechanisms underlying this finding should be investigated further to help determine the therapeutic action of this drug. PMID:23079823

  10. PCB153-Induced Overexpression of ID3 Contributes to the Development of Microvascular Lesions

    PubMed Central

    Das, Jayanta K.; Felty, Quentin

    2014-01-01

    Microvascular lesions resulting from endothelial cell dysfunction are produced in the brain, lung, kidney, and retina of patients of complex chronic diseases. The environmental and molecular risk factors which may contribute in the development of microvascular damage are unclear. The mechanism(s) responsible for initiating microvascular damage remain poorly defined, although several inciting factors have been proposed, including environmental toxicants-induced oxidative stress. Enhanced neovascularization has been implicated in either the development or progression of proliferative vascular lesions. Here, we present evidence for how PCB-induced ROS may contribute to the development of a neovascular phenotype with the aim of elucidating the role of environmental toxicants in endothelial dysfunction with a specific focus on the inhibitor of differentiation protein ID3. We used a combination of phenotype and immunohistochemical analysis followed by validating with protein expression and post-translational modifications with Western Blot and MALDI-TOF/TOF analysis. We also looked for a correlation between ID3 expression in vascular tissue. Our results showed that PCB-induced ROS mediated a highly tube branched neovascular phenotype that also depended on ID3 and Pyk2; and PCB153 treatment increased the size of endothelial spheroids under conditions typically used for clonal selection of stem cell spheroids. High ID3 protein expression correlated with a greater degree of malignancy and oxidative DNA damage marker 8-OHdG in blood vessels from human subjects. PCB153 treatment increased both serine and tyrosine phosphorylation of endothelial ID3. Stable ID3 overexpression increased cell survival of human microvascular endothelial cell line hCMEC/D3. In summary, our data provide evidence that ID3 may play a critical role in regulating vascular endothelial cell survival and development of microvascular lesions induced by persistent environmental pollutants such as PCB153

  11. Inhibitor of DNA binding 1 (Id1) induces differentiation and proliferation of mouse embryonic carcinoma P19CL6 cells

    SciTech Connect

    Meng, Qingzhen; Jia, Zhuqing; Wang, Weiping; Li, Binhong; Ma, Kangtao; Zhou, Chunyan

    2011-08-26

    Highlights: {yields} Id1 was upregulated during the cardiac differentiation process of P19CL6 cells. {yields} Id1 upregulated expression of cardiac specific genes Gata4, {alpha}-MHC and ISL1. {yields} Id1 promoted proliferation of P19CL6 cells. {yields} Overexpression of Id1 increased activity of TOP flash. {yields} Wnt3a or LiCl treatment promoted Id1 expression in P19CL6 cells. -- Abstract: The inhibitor of DNA binding (Id) family of genes encodes negative regulators of basic helix-loop-helix transcription factors and has been implicated in such diverse cellular processes as differentiation, proliferation, apoptosis and migration. Id knockout mouse embryos display multiple cardiac defects but the specific role of Id1 in cardiac differentiation is unclear. In the present study, we investigated the function of Id1 in DMSO-induced P19CL6 cells, a widely-accepted cell model of cardiac differentiation. We found that Id1 was upregulated during the cardiac differentiation of P19CL6 cells. The expression of cardiac specific marker genes, Gata4, {alpha}-MHC and ISL1, was upregulated in P19CL6 cells stably transfected with Id1 (P19CL6-Id1) during cardiac differentiation. The overexpression of Id1 reduced the number of cells in G1 phase and increased the cell population in G2, M and S phases, while knockdown of Id1 increased the number of cells in G1 phase from 48.6 {+-} 2.51% to 62.2 {+-} 1.52% at day 0 of cardiac induction, and from 52.5 {+-} 3.41% to 63.7 {+-} 1.02% at day 3 after cardiac induction, indicating that Id1 promoted proliferation of P19CL6 cells. Luciferase assays showed that the activity of TOP flash was higher in P19CL6-Id1 cells than wildtype P19CL6 cells, while Id1 expression was also upregulated in P19CL6 cells treated with Wnt3a or LiCl. This indicates that there may be positive feedback between Id1 and Wnt signaling which plays an important role in cardiac differentiation.

  12. Administration for Community Living

    MedlinePlus

    ... by Acting Assistant Secretary for Aging and ACL Administrator Edwin Walker at the HCBS Conference (08/29/ ... Remarks by Assistant Secretary on Aging and ACL Administrator Kathy Greenlee at the n4a Answers on Aging ...

  13. The New Administrative Computing.

    ERIC Educational Resources Information Center

    Green, Kenneth C.

    1988-01-01

    The past decade has seen dramatic changes in administrative computing, including more systems, more applications, a new group of computer users, and new opportunities for computer use in campus administration. (Author/MSE)

  14. Transportation Security Administration

    MedlinePlus

    ... content Official website of the Department of Homeland Security Transportation Security Administration When I fly can I bring my... ... to know if you could bring through the security checkpoint. Main menu Administrator Travel Security Screening Special ...

  15. Differential gene expression of FoxO1, ID1, and ID3 between young and older men and associations with muscle mass and function

    PubMed Central

    Buford, Thomas W.; Cooke, Matthew B.; Shelmadine, Brian D.; Hudson, Geoffrey M.; Redd, Liz L.; Willoughby, Darryn S.

    2013-01-01

    Background and aims Aging is associated with significant losses of skeletal muscle mass and function. Numerous biochemical molecules have been implicated in the development of these age-related changes, however evidence from human models is sparse. Assessment of transcript expression is useful as it requires minimal tissue and may potentially be used in clinical trials. This study aimed to compare mRNA expression of proteolytic genes in skeletal muscle of young (18–35 yrs) and older (55–75 yrs) men. Methods Muscle tissue was obtained from young (n=14, 21.35±1.03 yrs) and older (n=13, 63.85±1.83 yrs) men using percutaneous biopsy, and transcript expression was quantified using real-time polymerase chain reaction. Lower limb muscle mass was assessed using DEXA while concentric peak torque (PT) and power were assessed via isokinetic dynamometer. When age-related differences in mRNA expression were observed, Pearson correlation coefficients were obtained to examine the relationship of transcripts to muscle mass and function. Results Older muscle contained significantly more transcript for Forkhead Box O 1 (FoxO1, p=0.001), Inhibitor of DNA binding 1 (ID1, p=0.009), and Inhibitor of DNA Binding 3 (ID3, p=0.043) than young muscle. FoxO1 was significantly correlated with lean mass (R=−0.44, p=0.023) and PT (R=−0.40, p=0.046) while ID3 was significantly correlated with PT (R=−0.58, p=0.001) and power (R=−0.65, p<0.001). Moreover, ID1 was significantly correlated with all assessed measures of muscle function - mass (R=−0.39, p=0.046), PT (R=−0.53, p=0.005), and power (R=−0.520, p=0.005). Conclusion These data suggest that FoxO1, ID1, and ID3 are potentially useful as clinical biomarkers of age-related muscle atrophy and dysfunction. PMID:21993163

  16. Id-1 promotes osteosarcoma cell growth and inhibits cell apoptosis via PI3K/AKT signaling pathway.

    PubMed

    Hao, Liang; Liao, Qi; Tang, Qiang; Deng, Huan; Chen, Lu

    2016-02-12

    Accumulating evidence reveals that Id-1 is upregulated and functions as a potential tumor promoter in several human cancer types. However, the role of Id-1 in osteosarcoma (OS) is unknown. In present study, we found that Id-1 expression was elevated in OS tissues than adjacent normal bone tissues. More importantly, we demonstrated that overexpression of Id-1 is significantly correlated with tumor progression and poor survival in OS patients. Furthermore, increased expression of Id-1 was observed in OS cell lines and ectopic expression of Id-1 significantly enhanced in vitro cell proliferation and promoted in vivo tumor growth, whereas knockdown of Id-1 suppressed OS cells growth. Moreover, our experimental data revealed that Id-1 promotes cell proliferation by facilitating cell cycle progression and inhibits cell apoptosis. Mechanistically, the effects of Id-1 in OS cells is at least partly through activation of PI3K/Akt signaling pathway. Therefore, we identified a tumorigenic role of Id-1 in OS and suggested a potential therapeutic target for OS patients. PMID:26797271

  17. Id Proteins Contribute to Tumor Development and Metastatic Colonization in a Model of Bladder Carcinogenesis

    PubMed Central

    Garcia-Cao, Marta; Al-Ahmadie, Hikmat A.; Chin, Yvette; Bochner, Bernard H.; Benezra, Robert

    2015-01-01

    Abstract Background: Bladder cancer is one of the most common malignant genitourinary diseases worldwide. Despite advances in surgical technique, medical oncology and radiation therapy, cure of invasive tumors remains elusive for patients with late stage disease. Therefore, new therapeutic strategies are needed to improve the response rates with regard to recurrence, invasion and metastasis. Objective: Inhibitor of DNA binding (Id) proteins have been proposed as therapeutic targets due to the key regulatory role they exert in multiple steps of cancer. We aimed to explore the role of Id proteins in bladder cancer development and the pattern of expression of Id proteins in bladder carcinomas. Methods: We used a well-established chemically induced model of bladder carcinogenesis. Wild type and Id-deficient mice were given N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) in the drinking water and urinary bladder lesions were analyzed histopathologically and stained for Id1. We assessed the effects of Id1 inactivation in cultured bladder cancer cells and in a model of metastatic lung colonization. We also performed Id1 staining of human urothelial carcinoma samples and matched lymph node metastases. Results: Id1 protein was overexpressed in the BBN-induced model of bladder cancer. Id1 deficiency resulted in the development of urinary bladder tumors with areas of extensive hemorrhage and decreased invasiveness when compared to wild type mice. Id1 inactivation led to decreased cell growth in vitro and lung colonization in vivo of human bladder cancer cells. Immunohistochemistry performed on human urothelial carcinoma samples showed Id1 positive staining in both primary tumors and lymph node metastases. Conclusions: In summary, our studies reveal the physiological relevance of Id1 in bladder cancer progression and suggest that targeting Id1 may be important in the development of novel therapies for the treatment of bladder cancer.

  18. NASA, NOAA administrators nominated

    NASA Astrophysics Data System (ADS)

    Richman, Barbara T.

    President Ronald Reagan recently said he intended to nominate James Montgomery Beggs as NASA Administrator and John V. Byrne as NOAA Administrator. These two positions are key scientific posts that have been vacant since the start of the Reagan administration on January 20. The President also said he intends to nominate Hans Mark as NASA Deputy Administrator. At press time, Reagan had not designated his nominee for the director of the Office of Science and Technology Policy.

  19. A Philosophy of Administration.

    ERIC Educational Resources Information Center

    Bruening, William H.

    Justification is given for paying relatively large salaries to college administrators, specifically the president or chancellor and the chief academic officer. Three administrative task areas are discussed as criteria: management, administration per se, and leadership. It is contended that only leadership can be used as a criterion for…

  20. School Business Administration.

    ERIC Educational Resources Information Center

    Jordan, K. Forbis; And Others

    This textbook reviews the principal concerns within each of 13 major responsibility areas in school business administration. The first chapter assesses the political, social, and economic context in which schools function and school administrators work. The role and function of the school business administrator within this context is addressed in…