Sample records for administration tsa invites

  1. 76 FR 58532 - Intent To Request Renewal From OMB of One Current Public Collection of Information; TSA Customer...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-21

    ...The Transportation Security Administration (TSA) invites public comment on one currently approved Information Collection Request (ICR), Office of Management and Budget (OMB) control number 1652-0030 abstracted below that we will submit to OMB for renewal in compliance with the Paperwork Reduction Act (PRA). The ICR describes the nature of the information collection and its expected burden. This collection allows customers to provide feedback to TSA about their experiences with TSA's airport security process and procedures while traveling.

  2. 76 FR 44944 - Intent To Request Renewal From OMB of One Current Public Collection of Information: TSA Airspace...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-27

    ...The Transportation Security Administration (TSA) invites public comment on one currently approved Information Collection Request (ICR), Office of Management and Budget (OMB) control number 1652-0033, abstracted below that we will submit to OMB for renewal in compliance with the Paperwork Reduction Act (PRA). The ICR describes the nature of the information collection and its expected burden. This collection of information allows TSA to conduct security threat assessments on individuals on board aircraft operating in restricted airspace pursuant to an airspace waiver. This collection will enhance aviation security and protect assets on the ground that are within the restricted airspace.

  3. Oral and intraperitoneal administration of quercetin decreased lymphocyte DNA damage and plasma lipid peroxidation induced by TSA in vivo.

    PubMed

    Chan, Shu-Ting; Lin, Yi-Chin; Chuang, Cheng-Hung; Shiau, Rong-Jen; Liao, Jiunn-Wang; Yeh, Shu-Lan

    2014-01-01

    Our previous study showed that quercetin enhances the anticancer effect of trichostatin A (TSA) in xenograft mice given quercetin intraperitoneally (10 mg/kg, 3 times/week). Herein, we investigate whether quercetin administered orally exerts such an effect and prevents the cytotoxic side effects of TSA. We found that quercetin given orally (20 and 100 mg/kg, 3 times/week) failed to enhance the antitumor effect of TSA although it increased the total quercetin concentration more than quercetin administered intraperitoneally in the plasma. The compound quercetin-3-glucuronide (Q3G) increased the most. However, quercetin administered intraperitoneally increased the total quercetin level in tumor tissues more than oral quercetin. Oral and intraperitoneal administration of quercetin similarly decreased lymphocyte DNA damage and plasma lipid peroxidation level induced by TSA. Furthermore, we found that the enhancing effect of Q3G on the antitumor effect of TSA and the incorporation of Q3G was less than that of quercetin in A549 cells. However, we found that A549 cells possessed the ability to convert Q3G to quercetin. In conclusion, different from quercetin administered intraperitoneally, quercetin administered orally failed to enhance the antitumor effect of TSA because of its metabolic conversion. However, it prevented TSA-induced DNA damage and lipid peroxidation.

  4. TSA Prepares Students for Career Success

    ERIC Educational Resources Information Center

    Gupta, Pratyusha

    2012-01-01

    So often, people assume that TSA is only the Transportation Security Administration, but another very important TSA is the Technology Student Association, an extracurricular organization that uses competitive events and leadership to help develop science, technology, engineering, and math (STEM) skills and knowledge in its membership. In this…

  5. Oral and Intraperitoneal Administration of Quercetin Decreased Lymphocyte DNA Damage and Plasma Lipid Peroxidation Induced by TSA In Vivo

    PubMed Central

    Chan, Shu-Ting; Shiau, Rong-Jen; Liao, Jiunn-Wang; Yeh, Shu-Lan

    2014-01-01

    Our previous study showed that quercetin enhances the anticancer effect of trichostatin A (TSA) in xenograft mice given quercetin intraperitoneally (10 mg/kg, 3 times/week). Herein, we investigate whether quercetin administered orally exerts such an effect and prevents the cytotoxic side effects of TSA. We found that quercetin given orally (20 and 100 mg/kg, 3 times/week) failed to enhance the antitumor effect of TSA although it increased the total quercetin concentration more than quercetin administered intraperitoneally in the plasma. The compound quercetin-3-glucuronide (Q3G) increased the most. However, quercetin administered intraperitoneally increased the total quercetin level in tumor tissues more than oral quercetin. Oral and intraperitoneal administration of quercetin similarly decreased lymphocyte DNA damage and plasma lipid peroxidation level induced by TSA. Furthermore, we found that the enhancing effect of Q3G on the antitumor effect of TSA and the incorporation of Q3G was less than that of quercetin in A549 cells. However, we found that A549 cells possessed the ability to convert Q3G to quercetin. In conclusion, different from quercetin administered intraperitoneally, quercetin administered orally failed to enhance the antitumor effect of TSA because of its metabolic conversion. However, it prevented TSA-induced DNA damage and lipid peroxidation. PMID:24868531

  6. 76 FR 16799 - Intent To Request Renewal From OMB of One Current Public Collection of Information: TSA Claims...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-25

    ...The Transportation Security Administration (TSA) invites public comment on one currently approved Information Collection Request (ICR), Office of Management and Budget (OMB) control number 1652-0039, abstracted below that we will submit to OMB for renewal in compliance with the Paperwork Reduction Act (PRA). The ICR describes the nature of the information collection and its expected burden. The collection involves the submission of information from claimants in order to thoroughly examine and resolve tort claims against the agency.

  7. 7 CFR 1944.525 - Targeting of TSA funds to States.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 13 2010-01-01 2009-01-01 true Targeting of TSA funds to States. 1944.525 Section... § 1944.525 Targeting of TSA funds to States. (a) The Administrator will determine, based on the most... portion of the available funds for TSA to these States, leaving the balance available for national...

  8. The structure of the TsaB/TsaD/TsaE complex reveals an unexpected mechanism for the bacterial t6A tRNA-modification.

    PubMed

    Missoury, Sophia; Plancqueel, Stéphane; Li de la Sierra-Gallay, Ines; Zhang, Wenhua; Liger, Dominique; Durand, Dominique; Dammak, Raoudha; Collinet, Bruno; van Tilbeurgh, Herman

    2018-05-08

    The universal N6-threonylcarbamoyladenosine (t6A) modification at position A37 of ANN-decoding tRNAs is essential for translational fidelity. In bacteria the TsaC enzyme first synthesizes an l-threonylcarbamoyladenylate (TC-AMP) intermediate. In cooperation with TsaB and TsaE, TsaD then transfers the l-threonylcarbamoyl-moiety from TC-AMP onto tRNA. We determined the crystal structure of the TsaB-TsaE-TsaD (TsaBDE) complex of Thermotoga maritima in presence of a non-hydrolysable AMPCPP. TsaE is positioned at the entrance of the active site pocket of TsaD, contacting both the TsaB and TsaD subunits and prohibiting simultaneous tRNA binding. AMPCPP occupies the ATP binding site of TsaE and is sandwiched between TsaE and TsaD. Unexpectedly, the binding of TsaE partially denatures the active site of TsaD causing loss of its essential metal binding sites. TsaE interferes in a pre- or post-catalytic step and its binding to TsaBD is regulated by ATP hydrolysis. This novel binding mode and activation mechanism of TsaE offers good opportunities for antimicrobial drug development.

  9. 76 FR 71993 - Extension of Agency Information Collection Activity Under OMB Review: TSA Claims Management Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-21

    ... Information Collection Activity Under OMB Review: TSA Claims Management Program AGENCY: Transportation... Security Administration (TSA) has forwarded the Information Collection Request (ICR), Office of Management... the nature of the information collection and its expected burden. TSA published a Federal Register...

  10. Behaviour and biodegradation of sulfonamides (p-TSA, o-TSA, BSA) during drinking water treatment.

    PubMed

    Richter, Doreen; Massmann, Gudrun; Dünnbier, Uwe

    2008-04-01

    Three sulfonamides -para-toluenesulfonamide (p-TSA), ortho-toluenesulfonamide (o-TSA) and benzenesulfonamide (BSA) - have recently been detected in groundwater within a catchment area of one drinking water treatment plant (DWTP), which is located downstream of a former sewage farm. The degradation pathways of p-TSA, o-TSA and BSA were investigated during drinking water treatment with incubation experiments and an experimental filter. Incubation experiments showed that p-TSA is removed during the treatment by microbiological processes. Removal of p-TSA is performed by adapted microorganisms only present in polluted groundwater. The elimination in an experimental filter of 1.6m length applying filtration velocities from 2 to 6 m h(-1) was approximately 93% of p-TSA. The microbial degradation rates in the incubation experiment were approximately 0.029 microg l(-1) h(-1) (zero order reaction). In the experimental filter, the reaction rate constants were around 0.0063 s(-1) for all filtration velocities (1st order reaction). Drinking water treatment does not reduce the concentration of o-TSA and BSA under conditions encountered in Berlin. p-TSA, o-TSA and BSA were only measured in the low microg l(-1) concentrations range in the purified water.

  11. 49 CFR 1522.5 - TSA inspection authority.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... threats to transportation; (ii) Enforce security-related regulations, directives, and requirements: (iii... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY SECURITY RULES FOR ALL MODES OF TRANSPORTATION TSA-APPROVED VALIDATION FIRMS...

  12. 49 CFR 1520.15 - SSI disclosed by TSA or the Coast Guard.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY SECURITY RULES FOR ALL MODES OF TRANSPORTATION PROTECTION OF SENSITIVE SECURITY INFORMATION § 1520.15 SSI disclosed by TSA or the Coast Guard. (a) In... allegations contained in a legal enforcement action document issued by TSA or the Coast Guard. (2) Security...

  13. 76 FR 23326 - Intent To Request Renewal From OMB of One Current Public Collection of Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-26

    ... DEPARTMENT OF HOMELAND SECURITY Transportation Security Administration [Docket No. TSA-2006-24191... Notice. SUMMARY: The Transportation Security Administration (TSA) invites public comment on one currently... approved the collection of information for six months and TSA now seeks the maximum three-year approval...

  14. 49 CFR 1546.3 - TSA inspection authority.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY FOREIGN AIR CARRIER SECURITY General § 1546.3 TSA... inspections or tests, including copying records, to determine compliance of an airport operator, aircraft...

  15. 49 CFR 1546.3 - TSA inspection authority.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY FOREIGN AIR CARRIER SECURITY General § 1546.3 TSA... inspections or tests, including copying records, to determine compliance of an airport operator, aircraft...

  16. 49 CFR 1546.3 - TSA inspection authority.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY FOREIGN AIR CARRIER SECURITY General § 1546.3 TSA... inspections or tests, including copying records, to determine compliance of an airport operator, aircraft...

  17. 49 CFR 1546.3 - TSA inspection authority.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY FOREIGN AIR CARRIER SECURITY General § 1546.3 TSA... inspections or tests, including copying records, to determine compliance of an airport operator, aircraft...

  18. 49 CFR 1546.3 - TSA inspection authority.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY FOREIGN AIR CARRIER SECURITY General § 1546.3 TSA... inspections or tests, including copying records, to determine compliance of an airport operator, aircraft...

  19. 78 FR 4856 - Intent To Request Renewal From OMB of One Current Public Collection of Information: TSA Customer...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-23

    ... DEPARTMENT OF HOMELAND SECURITY Transportation Security Administration Intent To Request Renewal...: Transportation Security Administration, DHS. ACTION: 60-Day notice. SUMMARY: The Transportation Security... provide feedback to TSA about their experiences with TSA's airport security process and procedures while...

  20. 49 CFR 1548.3 - TSA inspection authority.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., foreign air carrier, indirect air carrier, or airport tenant with— (1) This subchapter, and any security... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY INDIRECT AIR CARRIER SECURITY § 1548.3 TSA inspection...

  1. 49 CFR 1548.3 - TSA inspection authority.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., foreign air carrier, indirect air carrier, or airport tenant with— (1) This subchapter, and any security... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY INDIRECT AIR CARRIER SECURITY § 1548.3 TSA inspection...

  2. 49 CFR 1548.3 - TSA inspection authority.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., foreign air carrier, indirect air carrier, or airport tenant with— (1) This subchapter, and any security... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY INDIRECT AIR CARRIER SECURITY § 1548.3 TSA inspection...

  3. 49 CFR 1548.3 - TSA inspection authority.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., foreign air carrier, indirect air carrier, or airport tenant with— (1) This subchapter, and any security... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY INDIRECT AIR CARRIER SECURITY § 1548.3 TSA inspection...

  4. 49 CFR 1548.3 - TSA inspection authority.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., foreign air carrier, indirect air carrier, or airport tenant with— (1) This subchapter, and any security... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY INDIRECT AIR CARRIER SECURITY § 1548.3 TSA inspection...

  5. TSA Should Stand for 'Thank Servicemembers Appropriately' | DoDLive

    Science.gov Websites

    civilians, Military Children, Transportation Security Administration, TSA. Bookmark the permalink. Comments children coalition forces Defense Department of Defense deployed deployment DoD DoD News families family

  6. 49 CFR 1503.101 - TSA requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false TSA requirements. 1503.101 Section 1503.101... Scope of Investigative and Enforcement Procedures § 1503.101 TSA requirements. (a) The investigative and enforcement procedures in this part apply to TSA's investigation and enforcement of violations of TSA...

  7. Indian TSA's: A Force for Community Service

    ERIC Educational Resources Information Center

    American Indian Journal, 1978

    1978-01-01

    Assisting tribal governments in meeting the needs of their members, the Kiowa Tribe, the Institute for the Development of Indian Law, and the National Paralegal Institute sponsored the first Tribal Service Advisor training event this year (TSA's can represent clients at the administrative level in many legal and social welfare areas). (JC)

  8. 77 FR 31866 - Intent To Request Approval From OMB of One New Public Collection of Information: Baseline...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-30

    ... (BASE) Program for Public Transportation Systems AGENCY: Transportation Security Administration, DHS. ACTION: 60-day Notice. SUMMARY: The Transportation Security Administration (TSA) invites public comment... under contract with a public transportation agency. \\1\\ TSA, ``Transportation Sector-Specific Plan Mass...

  9. 78 FR 37561 - Extension of Agency Information Collection Activity Under OMB Review: TSA Customer Comment Card

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-21

    ...This notice announces that the Transportation Security Administration (TSA) has forwarded the Information Collection Request (ICR), Office of Management and Budget (OMB) control number 1652-0030, abstracted below to OMB for review and approval of an extension of the currently approved collection under the Paperwork Reduction Act (PRA). The ICR describes the nature of the information collection and its expected burden. TSA published a Federal Register notice, with a 60-day comment period soliciting comments, of the following collection of information on January 23, 2013 (78 FR 4856). This collection allows customers to provide feedback to TSA about their experiences with TSA's airport security process and procedures while traveling.

  10. 78 FR 59363 - New Agency Information Collection Activity Under OMB Review: TSA Pre✓TM

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-26

    ...This notice announces that the Transportation Security Administration (TSA) has forwarded the new Information Collection Request (ICR) abstracted below to the Office of Management and Budget (OMB) for review and approval under the Paperwork Reduction Act (PRA). The ICR describes the nature of the information collection and its expected burden. TSA published a Federal Register notice, with a 60-day comment period soliciting comments, of the following collection of information on July 23, 2013 (78 FR 44140). The collection involves the submission of biographic and biometric information by individuals seeking to enroll in the TSA Pre[check]TM Application Program.

  11. Identification and significance of sulphonamides (p-TSA, o-TSA, BSA) in an urban water cycle (Berlin, Germany).

    PubMed

    Richter, Doreen; Massmann, Gudrun; Dünnbier, Uwe

    2008-03-01

    Because of the nature of the water cycle in Berlin, a number of persistent wastewater residues are present in the surface water and may potentially reach the groundwater via bank filtration and artificial recharge. The occurrence and behaviour of the wastewater residues para-toluenesulphonamide (p-TSA), ortho-toluenesulphonamide (o-TSA) and benzenesulphonamide (BSA) through wastewater treatment, surface water, bank filtration and drinking water treatment was studied. In addition, groundwater below a former sewage farm was investigated. All three compounds are ubiquitous in the aquatic environment of Berlin. p-TSA concentrations are much higher than those of o-TSA and BSA. p-TSA was found in high concentrations in the wastewater influent (2 to 15microg/L), in the wastewater effluent (<0.15 to 2.34microg/L) and in sewage farm groundwater (<0.05 to 20microg/L) and in lower concentrations in the surface water (<0.05 to 1.15microg/L), bank filtrate (<0.05 to 0.30microg/L) and drinking water (<0.05 to 0.54microg/L). p-TSA is considerably depleted during waste- and drinking water treatment ( approximately 90% reduction each). The concentration ranges for o-TSA and BSA in wastewater influents were 0.11 to 8microg/L and <0.05 to 0.64microg/L, respectively, while the values for wastewater effluents were 0.14 to 4microg/L for o-TSA and 0.25 to 0.49microg/L for BSA. Wastewater treatment and drinking water treatment do not reduce the concentrations of o-TSA and BSA. The behaviour of o-TSA during wastewater treatment varies largely between different wastewater treatment plants where concentrations increase, remain constant or decrease. BSA forms during treatment. The concentrations measured in surface water, sewage farm groundwater, bank filtrate and drinking water were <0.05 to 1.74microg/L for o-TSA and <0.05 to 0.53microg/L for BSA.

  12. 7 CFR 1944.540 - Requesting TSA checks.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 13 2010-01-01 2009-01-01 true Requesting TSA checks. 1944.540 Section 1944.540... TSA checks. (a) The initial TSA check may cover the applicant's needs for the first calendar month. If... the next whole month. (b) The initial advance of TSA grant funds may not be requested simultaneously...

  13. 49 CFR 1550.3 - TSA inspection authority.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false TSA inspection authority. 1550.3 Section 1550.3... RULES § 1550.3 TSA inspection authority. (a) Each aircraft operator subject to this part must allow TSA..., and part 1520 of this chapter; and (2) 49 U.S.C. Subtitle VII, as amended. (b) At the request of TSA...

  14. 49 CFR 1544.3 - TSA inspection authority.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... records. (c) TSA may enter and be present within secured areas, AOAs, SIDAs, and other areas where... other such duties as TSA may direct. (d) At the request of TSA and the completion of SIDA training as...

  15. 49 CFR 1544.3 - TSA inspection authority.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... records. (c) TSA may enter and be present within secured areas, AOAs, SIDAs, and other areas where... other such duties as TSA may direct. (d) At the request of TSA and the completion of SIDA training as...

  16. 49 CFR 1544.3 - TSA inspection authority.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... records. (c) TSA may enter and be present within secured areas, AOAs, SIDAs, and other areas where... other such duties as TSA may direct. (d) At the request of TSA and the completion of SIDA training as...

  17. 49 CFR 1544.3 - TSA inspection authority.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... records. (c) TSA may enter and be present within secured areas, AOAs, SIDAs, and other areas where... other such duties as TSA may direct. (d) At the request of TSA and the completion of SIDA training as...

  18. 49 CFR 1544.3 - TSA inspection authority.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... records. (c) TSA may enter and be present within secured areas, AOAs, SIDAs, and other areas where... other such duties as TSA may direct. (d) At the request of TSA and the completion of SIDA training as...

  19. A Study of the Utility of Results from the 1992 Trial State Assessment (TSA) in Reading for State-Level Administrators of Assessment.

    ERIC Educational Resources Information Center

    Bullock, Cheryl Davis; DeStefano, Lizanne

    1998-01-01

    The usefulness of the 1992 TSA in reading was studied using interviews from 26 state directors of assessment. Perceptions about TSA credibility and orientation of test components and current use of TSA results were examined. The directors suggested involving more teachers in assessment, modifying descriptors, disseminating results quicker, and…

  20. 77 FR 42082 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel GUILDING LIGHT; Invitation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-17

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2012 0079] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel GUILDING LIGHT; Invitation for Public Comments AGENCY... GUILDING LIGHT is: [[Page 42083

  1. A Value Measure for Public-Sector Enterprise Risk Management: A TSA Case Study.

    PubMed

    Fletcher, Kenneth C; Abbas, Ali E

    2018-05-01

    This article presents a public value measure that can be used to aid executives in the public sector to better assess policy decisions and maximize value to the American people. Using Transportation Security Administration (TSA) programs as an example, we first identify the basic components of public value. We then propose a public value account to quantify the outcomes of various risk scenarios, and we determine the certain equivalent of several important TSA programs. We illustrate how this proposed measure can quantify the effects of two main challenges that government organizations face when conducting enterprise risk management: (1) short-term versus long-term incentives and (2) avoiding potential negative consequences even if they occur with low probability. Finally, we illustrate how this measure enables the use of various tools from decision analysis to be applied in government settings, such as stochastic dominance arguments and certain equivalent calculations. Regarding the TSA case study, our analysis demonstrates the value of continued expansion of the TSA trusted traveler initiative and increasing the background vetting for passengers who are afforded expedited security screening. © 2017 Society for Risk Analysis.

  2. 77 FR 31432 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel PASSION; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-25

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD 2012 0062] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel PASSION; Invitation for Public Comments AGENCY... PASSION is: Intended Commercial Use of Vessel: ``Bareboat chartering, sailing classes.'' Geographic Region...

  3. 76 FR 75948 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel CHRYSALIS; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-05

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2011 0149] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel CHRYSALIS; Invitation for Public Comments AGENCY... CHRYSALIS is: Intended Commercial Use of Vessel: ``Weekly charter vessel.'' Geographic Region: ``Florida...

  4. 77 FR 40146 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SATISFACTION; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-06

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2012-0076] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SATISFACTION; Invitation for Public Comments AGENCY... SATISFACTION is: Intended Commercial Use of Vessel: ``Sightseeing, whale watching, snorkeling, and cruising...

  5. TSA - A Two Scale Approximation for Wind-Generated Ocean Surface Waves

    DTIC Science & Technology

    2012-09-30

    broad-scale version of TSA, or ‘ dTSA ’. In this manner dTSA is able to respond to changing wind situations. Results were shown to compare well with ‘exact...We also implemented the revised version of TSA, denoted ‘ dTSA ’, in WW3 for tests with a storm case, hurricane Juan, which made landfall as a...manner in which the broad-scale of TSA was defined, developing ‘ dTSA ’ as described above, so that in complicated rapidly changing wave spectra cases, a

  6. 78 FR 39061 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel MISTRESS MALLIKA; Invitation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-28

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0076] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel MISTRESS MALLIKA; Invitation for Public Comments AGENCY... MISTRESS MALLIKA is: Intended Commercial Use Of Vessel: ``Pleasure Charters''. Geographic Region: Rhode...

  7. 78 FR 25529 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel ALCHEMY; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-01

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0048] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel ALCHEMY; Invitation for Public Comments AGENCY... ALCHEMY is: Intended Commercial Use of Vessel: ``Uninspected six-pack sailing charters and instruction...

  8. 78 FR 25531 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel TEMPEST; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-01

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013-0046] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel TEMPEST; Invitation for Public Comments AGENCY... TEMPEST is: Intended Commercial Use of Vessel: ``Offshore wreck diving.'' Geographic Region: Maine, New...

  9. 78 FR 47825 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SERENITY; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-06

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0091] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SERENITY; Invitation for Public Comments AGENCY... SERENITY is: Intended Commercial Use of Vessel: ``Coastwise charters, Sight seeing tours, sunset and...

  10. 78 FR 20172 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BASIC INSTINCT; Invitation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-03

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0037] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BASIC INSTINCT; Invitation for Public Comments AGENCY... BASIC INSTINCT is: Intended Commercial Use Of Vessel: Charters for day excursions. Geographic Region...

  11. 78 FR 46410 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel IMPOSSIBLE DREAM; Invitation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-31

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0084] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel IMPOSSIBLE DREAM; Invitation for Public Comments AGENCY... of the vessel IMPOSSIBLE DREAM is: Intended Commercial Use of Vessel: ``Day, Multiple Day, Week...

  12. ERK inhibition enhances TSA-induced gastric cancer cell apoptosis via NF-κB-dependent and Notch-independent mechanism.

    PubMed

    Yao, Jun; Qian, Cui-Juan; Ye, Bei; Zhang, Xin; Liang, Yong

    2012-09-04

    To analyze the combined impact of the histone deacetylase inhibitor (HDACI) Trichostatin A (TSA) and the extracellular-signal-regulated kinase 1/2 (ERK1/2) inhibitor PD98059 on gastric cancer (GC) cell line SGC7901 growth. SGC7901 cells were treated with TSA, PD98059 or with a TSA-PD98059 combination. Effects of drug treatment on tumor cell proliferation, apoptosis, cell cycle progression, and cell signaling pathways were investigated by MTS assay, flow cytometry, Western blotting, chromatin immunoprecipitation (ChIP) assay, electrophoretic mobility shift assay (EMSA), and luciferase reporter assay, respectively. PD98059 enhanced TSA-induced cell growth arrest, apoptosis and activation of p21(WAF1/CIP1), but reversed TSA-induced activation of ERK1/2 and nuclear factor-κB (NF-κB). TSA alone up-regulated Notch1 and Hes1, and down-regulated Notch2, but PD98059 did not affect the trends of Notch1 and Notch2 induced by TSA. Particularly, PD98059 did potentiate the ability of TSA to down-regulate phospho-histone H3 protein, but increased levels of the acetylated forms of histone H3 bound to the p21(WAF1/CIP1) promoter, leading to enhanced expression of p21(WAF1/CIP1) in SGC7901 cells. PD98059 synergistically potentiates TSA-induced GC growth arrest and apoptosis by manipulating NF-κB and p21(WAF1/CIP1) independent of Notch. Therefore, concomitant administration of HDACIs and ERK1/2 inhibitors may be a promising treatment strategy for individuals with GC. Copyright © 2012 Elsevier Inc. All rights reserved.

  13. 76 FR 70469 - Extension of Agency Information Collection Activity Under OMB Review: TSA Airspace Waiver Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-14

    ...This notice announces that the Transportation Security Administration (TSA) has forwarded the Information Collection Request (ICR), Office of Management and Budget (OMB) control number 1652-0033, abstracted below to OMB for review and approval of an extension of the currently approved collection under the Paperwork Reduction Act (PRA). The ICR describes the nature of the information collection and its expected burden. TSA published a Federal Register notice, with a 60-day comment period soliciting comments, of the following collection of information on July 27, 2011, 76 FR 44944. This collection of information allows TSA to conduct security threat assessments on individuals on board aircraft operating in restricted airspace pursuant to an airspace waiver. This collection will enhance aviation security and protect assets on the ground that are within the restricted airspace.

  14. 77 FR 37062 - Intent To Request Approval From OMB of One New Public Collection of Information: Office of Law...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-20

    ... DEPARTMENT OF HOMELAND SECURITY Transportation Security Administration Intent To Request Approval... Service LEO Reimbursement Request AGENCY: Transportation Security Administration, DHS. ACTION: 60-day Notice. SUMMARY: The Transportation Security Administration (TSA) invites public comment on a new...

  15. 76 FR 79764 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel DREAM CATCHER; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-22

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2011-0156] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel DREAM CATCHER; Invitation for Public Comments AGENCY... DREAM CATCHER is: INTENDED COMMERCIAL USE OF VESSEL: ``Passenger charter.'' GEOGRAPHIC REGION: ``Georgia...

  16. 77 FR 20100 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel NORDIC STAR; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-03

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2012 0039] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel NORDIC STAR; Invitation for Public Comments AGENCY... NORDIC STAR is: Intended Commercial Use of Vessel: ``Sailing excursions and extended charters...

  17. 76 FR 67254 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel ABOUT TIME; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-31

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD 2011 0136] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel ABOUT TIME; Invitation for Public Comments AGENCY... ABOUT TIME is: INTENDED COMMERCIAL USE OF VESSEL: ``Uninspected passenger vessel.'' GEOGRAPHIC REGION...

  18. 78 FR 19066 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel MI CASA; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-28

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0035] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel MI CASA; Invitation for Public Comments AGENCY... CASA is: Intended Commercial Use of Vessel: Passenger charters. Geographic Region: Massachusetts, New...

  19. 78 FR 70096 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BLUE MOON; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-22

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013-0129] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BLUE MOON; Invitation for Public Comments AGENCY... MOON is: Intended Commercial Use of Vessel: ``Bareboat Charter'' Geographic Region: ``Maine, Maryland...

  20. 77 FR 35743 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SECOND CHANCE; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-14

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD 2012 0067] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SECOND CHANCE; Invitation for Public Comments AGENCY... SECOND CHANCE is: Intended Commercial Use of Vessel: ``Small group charters.'' Geographic Region...

  1. 78 FR 35092 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BEAR BOAT; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-11

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013-0069] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BEAR BOAT; Invitation for Public Comments AGENCY... BOAT is: Intended Commercial Use of Vessel: ``Crewed and unscrewed (bareboat) sailboat charters...

  2. 77 FR 56264 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel HAPPY ENDINGS; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-12

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD 2012 0090] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel HAPPY ENDINGS; Invitation for Public Comments AGENCY... of the vessel HAPPY ENDINGS is: Intended Commercial Use of Vessel: ``Sunset, day and overnight...

  3. 77 FR 5628 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BIG GAME; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-03

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2012-0003] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BIG GAME; Invitation for Public Comments AGENCY... GAME is: Intended Commercial Use of Vessel: ``Fishing charter boat.'' Geographic Region: ``Rhode Island...

  4. 78 FR 25530 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BLUE DOLPHIN; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-01

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013-0049] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BLUE DOLPHIN; Invitation for Public Comments AGENCY....gov . SUPPLEMENTARY INFORMATION: As described by the applicant the intended service of the vessel BLUE...

  5. 78 FR 76406 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel COSMO; Invitation for Public...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-17

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013-0142] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel COSMO; Invitation for Public Comments AGENCY: Maritime... COSMO is: Intended Commercial Use Of Vessel: ``Day sail charters and lessons.'' Geographic Region: ``New...

  6. 78 FR 35093 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel EYE DOC; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-11

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0071] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel EYE DOC; Invitation for Public Comments AGENCY....gov . SUPPLEMENTARY INFORMATION: As described by the applicant the intended service of the vessel EYE...

  7. 78 FR 53004 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SECOND WIND; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-27

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD 2013 0093] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SECOND WIND; Invitation for Public Comments AGENCY... SECOND WIND is: Intended Commercial Use of Vessel: ``Breakfast, lunch, and dinner cruises featuring...

  8. 78 FR 70097 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BEE; Invitation for Public...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-22

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0128] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BEE; Invitation for Public Comments AGENCY: Maritime....gov . SUPPLEMENTARY INFORMATION: As described by the applicant the intended service of the vessel BEE...

  9. 78 FR 23823 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SEA HAG; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-22

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013-0042] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SEA HAG; Invitation for Public Comments AGENCY....gov . SUPPLEMENTARY INFORMATION: As described by the applicant the intended service of the vessel SEA...

  10. 78 FR 8698 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel COOL BEANS; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-06

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0005] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel COOL BEANS; Invitation for Public Comments AGENCY... BEANS is: Intended Commercial Use of Vessel: Sightseeing and sunset cruises. Geographic Region: Florida...

  11. 78 FR 42153 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel COMPASS ROSE; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-15

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013-0081] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel COMPASS ROSE; Invitation for Public Comments AGENCY... COMPASS ROSE is: Intended Commercial Use Of Vessel: ``Sailboat charters six passengers or less...

  12. 76 FR 67253 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel THE GIFT; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-31

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD 2011 0131] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel THE GIFT; Invitation for Public Comments AGENCY... GIFT is: INTENDED COMMERCIAL USE OF VESSEL: ``Passenger carrying.'' GEOGRAPHIC REGION: ``ME, NH, MA, RI...

  13. 77 FR 26818 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel FREE SPIRIT; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-07

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2012-0058] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel FREE SPIRIT; Invitation for Public Comments AGENCY... SPIRIT is: Intended Commercial Use of Vessel: ``Day/overnight passenger passage. Depart and return same...

  14. 77 FR 74274 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel MELLO MOON; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-13

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2012-0110] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel MELLO MOON; Invitation for Public Comments AGENCY... of the vessel MELLO MOON is: Intended Commercial Use of Vessel: ``Six pack passenger charters...

  15. 77 FR 10802 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SILVER MOON; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-23

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD 2012 0012] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SILVER MOON; Invitation for Public Comments AGENCY... SILVER MOON is: Intended Commercial Use of Vessel: ``Catamaran Sailing Charters, both term and day...

  16. 78 FR 76403 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel JAGUAR; Invitation for Public...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-17

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0146] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel JAGUAR; Invitation for Public Comments AGENCY: Maritime... JAGUAR is: Intended Commercial Use Of Vessel: ``For bare boat/lease recreational use.'' Geographic Region...

  17. 76 FR 70216 - Requested Administrative Waiver of the Coastwise Trade Laws; Vessel REEL ATTITUDE; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-10

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2011 0146] Requested Administrative Waiver of the Coastwise Trade Laws; Vessel REEL ATTITUDE; Invitation for Public Comments AGENCY... ATTITUDE is: Intended Commercial Use of Vessel: ``Fishing Charter passenger less than 6.'' Geographic...

  18. 78 FR 40265 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel HMS-DISCOVERY; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-03

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0077] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel HMS-DISCOVERY; Invitation for Public Comments AGENCY... of the vessel HMS-DISCOVERY is: Intended Commercial Use of Vessel: ``Sight Seeing Tours''. Geographic...

  19. 78 FR 33150 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BLACK ICE; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-03

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0063] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BLACK ICE; Invitation for Public Comments AGENCY... BLACK ICE is: Intended Commercial Use Of Vessel: Small passenger sails. Sightseeing, dinner sails...

  20. A Layman's Guide to Understanding TSA Compliance.

    ERIC Educational Resources Information Center

    Alioto, Nicholas C. A.; Simandl, Robert J.

    1998-01-01

    Since school district business offices are ill-equipped to administer tax shelter annuity (TSA) plans, the result has been inadequate legal compliance and significant financial exposure. This article explains the rules governing TSA planning (testing limitations), examines common pitfalls for districts conducting audits, and outlines steps for…

  1. Strategic Improvements to TSA Spot Program

    DTIC Science & Technology

    2015-03-01

    Willard Shepard , “TSA Agents Rescue Kidnapped Woman,” NBC 6 South Florida. July 31, 2012, http://www.nbcmiami.com/news/local/Kidnapped-Woman-Was...For example, a high percentage of Muslims being selected by the BDOs for additional screening at a Chicago airport does not necessarily indicate...Muslim.109 Further, the traveling population from Chicago airports could have an even higher Muslim population. Local TSA

  2. 76 FR 67255 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel KIWI CAT; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-31

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD 2011 0135] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel KIWI CAT; Invitation for Public Comments AGENCY... CAT is: INTENDED COMMERCIAL USE OF VESSEL: ``Small vessel 12 person chartering service for the San...

  3. 78 FR 23823 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel FISHIN GAME; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-22

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0043] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel FISHIN GAME; Invitation for Public Comments AGENCY... FISHIN GAME is: Intended Commercial Use Of Vessel: Sport fishing excursions with six passengers or less...

  4. 78 FR 13752 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel GATO; Invitation for Public...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0012] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel GATO; Invitation for Public Comments AGENCY: Maritime... . SUPPLEMENTARY INFORMATION: As described by the applicant the intended service of the vessel GATO is: Intended...

  5. 78 FR 37659 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel ORCA; Invitation for Public...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-21

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0075] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel ORCA; Invitation for Public Comments AGENCY: Maritime... . SUPPLEMENTARY INFORMATION: As described by the applicant the intended service of the vessel ORCA is: Intended...

  6. 77 FR 73735 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel PREDATOR II; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-11

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2012-0108] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel PREDATOR II; Invitation for Public Comments AGENCY... of the vessel PREDATOR II is: Intended Commercial Use of Vessel: 6 pack private charters. Geographic...

  7. 78 FR 13751 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel LAST TIME AROUND; Invitation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0015] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel LAST TIME AROUND; Invitation for Public Comments AGENCY... TIME AROUND is: Intended Commercial Use Of Vessel: ``Chartering for day or week'' Geographic Region...

  8. 78 FR 13752 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SPIRIT; Invitation for Public...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0013] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SPIRIT; Invitation for Public Comments AGENCY: Maritime... . SUPPLEMENTARY INFORMATION: As described by the applicant the intended service of the vessel SPIRIT is: Intended...

  9. 77 FR 22631 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SPIRIT; Invitation for Public...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-16

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD 2012 0049] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SPIRIT; Invitation for Public Comments AGENCY: Maritime... . SUPPLEMENTARY INFORMATION: As described by the applicant the intended service of the vessel SPIRIT is: Intended...

  10. 76 FR 76812 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BARBARY GHOST; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-08

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD 2011 0148] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BARBARY GHOST; Invitation for Public Comments AGENCY... BARBARY GHOST is: Intended Commercial Use Of Vessel: ``Sightseeing tours in the San Francisco Bay for a...

  11. 78 FR 14413 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel LUCKY DUCK; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-05

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0019] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel LUCKY DUCK; Invitation for Public Comments AGENCY... LUCKY DUCK is: Intended Commercial Use Of Vessel: ``The vessel is to be operated as a sailing...

  12. 78 FR 30961 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel LITTLE DUTCH; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-23

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013-0057] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel LITTLE DUTCH; Invitation for Public Comments AGENCY... LITTLE DUTCH is: Intended Commercial Use of Vessel: ``Carrying up to six passengers for day trips, sunset...

  13. 77 FR 26819 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BLUE PLANET; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-07

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2012-0054] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BLUE PLANET; Invitation for Public Comments AGENCY... PLANET is: Intended Commercial use of Vessel: ``Charter to Boy Scouts of America Florida Sea Base...

  14. 49 CFR 1549.3 - TSA inspection authority.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... transportation facilities; (vi) Review security plans; and (vii) Carry out such other duties, and exercise such... access media or identification media issued or approved by a certified cargo screening facility or other person, except that the TSA and DHS officials will have identification media issued by TSA or DHS. ...

  15. [TSA improve transgenic porcine cloned embryo development and transgene expression].

    PubMed

    Kong, Qing-Ran; Zhu, Jiang; Huang, Bo; Huan, Yan-Jun; Wang, Feng; Shi, Yong-Qian; Liu, Zhong-Feng; Wu, Mei-Ling; Liu, Zhong-Hua

    2011-07-01

    Uncompleted epigenetic reprogramming is attributed to the low efficiency of producing transgenic cloned animals. Histone modification associated with epigenetics can directly influence the embryo development and transgene expression. Trichostatin A (TSA), as an inhibitor of histone deacetylase, can change the status of histone acetylation, improve somatic cell reprogramming, and enhance cloning efficiency. TSA prevents the chromatin structure from being condensed, so that transcription factor could binds to DNA sequence easily and enhance transgene expression. Our study established the optimal TSA treatment on porcine donor cells and cloned embryos, 250 nmol/L, 24 h and 40 nmol/L, 24 h, respectively. Furthermore, we found that both the cloned embryo and the donor cell treated by TSA resulted in the highest development efficiency. Meanwhile, TSA can improve transgene expression in donor cell and cloned embryo. In summary, TSA can significantly improve porcine reconstructed embryo development and transgene expression.

  16. 77 FR 35745 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SMOKE AND ROSES; Invitation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-14

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD 2012 0068] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SMOKE AND ROSES; Invitation for Public Comments AGENCY... SMOKE AND ROSES is: INTENDED COMMERCIAL USE OF VESSEL: ``We intend to carry up to 10 passengers for hire...

  17. Traditional serrated adenoma (TSA): morphological questions, queries and quandaries.

    PubMed

    Chetty, Runjan

    2016-01-01

    Traditional serrated adenoma (TSA) is an uncommon type of serrated adenoma that can be a precursor to biologically aggressive colorectal cancer that invokes the serrated (accelerated) pathway. The purpose of this review is to address some of the more contentious issues around nomenclature, diagnostic criteria, histological variants, coexistence with other polyp types, the occurrence of dysplasia and the differential diagnosis. While the vast majority of TSAs are exophytic villiform polyps composed of deeply eosinophilic cells, flat top luminal serrations and numerous ectopic crypt foci, histological variants include flat TSA, filiform TSA and one composed of large numbers of mucin-containing cells. It is unlikely that there is any biological difference between the histological variants. There is a contention that TSAs are not dysplastic ab initio and that the majority do not show cytological atypia. Two types of dysplasia are associated with TSA. Serrated dysplasia is less well recognised and less commonly encountered than adenomatous dysplasia. TSA with dysplasia must be separated from TSA with coexisting conventional adenoma. TSA is a characteristic polyp that may be extremely exophytic, flat or composed of mucin-rich cells and is typified by numerous ectopic crypt foci. They may coexist with other serrated polyps and conventional adenomas. Approximately 20-25% will be accompanied by adenomatous dysplasia. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

  18. Autotaxin is induced by TSA through HDAC3 and HDAC7 inhibition and antagonizes the TSA-induced cell apoptosis.

    PubMed

    Li, Song; Wang, Baolu; Xu, Yan; Zhang, Junjie

    2011-02-12

    Autotaxin (ATX) is a secreted glycoprotein with the lysophospholipase D (lysoPLD) activity to convert lysophosphatidylcholine (LPC) into lysophosphatidic acid (LPA), a bioactive lysophospholipid involved in diverse biological actions. ATX is highly expressed in some cancer cells and contributes to their tumorigenesis, invasion, and metastases, while in other cancer cells ATX is silenced or expressed at low level. The mechanism of ATX expression regulation in cancer cells remains largely unknown. In the present study, we demonstrated that trichostatin A (TSA), a well-known HDAC inhibitor (HDACi), significantly induced ATX expression in SW480 and several other cancer cells with low or undetectable endogenous ATX expression. ATX induction could be observed when HDAC3 and HDAC7 were down-regulated by their siRNAs. It was found that HDAC7 expression levels were low in the cancer cells with high endogenous ATX expression. Exogenous over-expression of HDAC7 inhibited ATX expression in these cells in a HDAC3-dependent manner. These data indicate that HDAC3 and HDAC7 collaboratively suppress ATX expression in cancer cells, and suggest that TSA induce ATX expression by inhibiting HDAC3 and HDAC7. The biological significance of this regulation mechanism was revealed by demonstrating that TSA-induced ATX protected cancer cells against TSA-induced apoptosis by producing LPA through its lysoPLD activity, which could be reversed by BrP-LPA and S32826, the inhibitors of the ATX-LPA axis. We have demonstrated that ATX expression is repressed by HDAC3 and HDAC7 in cancer cells. During TSA treatment, ATX is induced due to the HDAC3 and HDAC7 inhibition and functionally antagonizes the TSA-induced apoptosis. These results reveal an internal HDACi-resistant mechanism in cancer cells, and suggest that the inhibition of ATX-LPA axis would be helpful to improve the efficacy of HDACi-based therapeutics against cancer.

  19. 77 FR 56264 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel TREE OF LIFE; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-12

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD 2012 0089] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel TREE OF LIFE; Invitation for Public Comments AGENCY... OF LIFE is: Intended Commercial Use of Vessel: ``Passenger carrying charter vessel.'' Geographic...

  20. 76 FR 76813 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel FOR-2-NA; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-08

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2011 0157] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel FOR-2-NA; Invitation for Public Comments AGENCY...-2-NA is: Intended Commercial Use of Vessel: ``Charter fishing, for hire.'' Geographic Region...

  1. 78 FR 19068 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel GOLDEN BOY II; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-28

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0036] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel GOLDEN BOY II; Invitation for Public Comments AGENCY... GOLDEN BOY II is: Intended Commercial Use Of Vessel: Limited charter of passengers for luxury day...

  2. 78 FR 39062 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SEA BREEZE 27; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-28

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0079] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SEA BREEZE 27; Invitation for Public Comments AGENCY....gov . SUPPLEMENTARY INFORMATION: As described by the applicant the intended service of the vessel SEA...

  3. 77 FR 26820 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel THE BLUE MOON; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-07

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2012-0055] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel THE BLUE MOON; Invitation for Public Comments AGENCY... BLUE MOON is: Intended Commercial Use of Vessel: ``6 pack UPV (short cruises).'' Geographic Region...

  4. 77 FR 66911 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel FIVE O'CLOCK HERE; Invitation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-07

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2012-0102] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel FIVE O'CLOCK HERE; Invitation for Public Comments... O'CLOCK HERE is: Intended Commercial Use of Vessel: ``Day and overnight charters in California...

  5. Technology Education Curriculum Guide for Connecticut-AIASA/TSA.

    ERIC Educational Resources Information Center

    Monty, Phil

    This curriculum guide is designed to provide advisors with ways to manage technology student activities within their American Industrial Arts Student Association/Technology Student Association (AIASA/TSA) program. Section 1 focuses on how to organize a local AIASA/TSA chapter. It covers organizing procedures organizational meetings, chapter…

  6. 78 FR 19362 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel W.L. STEWART III; Invitation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-29

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0026] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel W.L. STEWART III; Invitation for Public Comments AGENCY....gov . SUPPLEMENTARY INFORMATION: As described by the applicant the intended service of the vessel W.L...

  7. 77 FR 21150 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SIR MARTIN II; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-09

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD 2012 0047] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SIR MARTIN II; Invitation for Public Comments AGENCY... the vessel SIR MARTIN II is: Intended Commercial Use of Vessel: ``Day charter up to 6 passengers for...

  8. Sessile serrated adenoma (SSA) vs. traditional serrated adenoma (TSA).

    PubMed

    Torlakovic, Emina Emilia; Gomez, Jose D; Driman, David K; Parfitt, Jeremy R; Wang, Chang; Benerjee, Tama; Snover, Dale C

    2008-01-01

    The morphologic distinction between various serrated polyps of the colorectum may be challenging. The distinction between sessile serrated adenoma (SSA) and traditional serrated adenoma (TSA) may be difficult using currently available criteria mostly based on cytologic characteristics. We have evaluated 66 serrated polyps including 29 SSA, 18 TSA, and 19 hyperplastic polyps for overall shape of the polyps, architectural features of individual crypts, the presence of eosinophilic cytoplasm, size and distribution of the proliferation and maturation zones, as well as Ki-67 and CK20 expression. The extent of the expression of CK20 and Ki-67 could not distinguish between the 3 types of serrated polyps, but the distribution of their expression was very helpful and differences were statistically significant. The distribution of Ki-67+ cells was the single most helpful distinguishing feature of the serrated polyp type (P<0.0001, chi test). Hyperplastic polyps had regular, symmetric, and increased Ki-67 expression. SSA had irregular, asymmetric, and highly variable expression of Ki-67. TSA had low Ki-67 expression, which was limited to "ectopic crypts" and admixed tubular adenomalike areas. In serrated polyps, ectopic crypt formation (ECF) defined by the presence of ectopic crypts with their bases not seated adjacent to the muscularis mucosae was nearly exclusive to TSA and was found in all cases, while the presence of cytologic atypia and eosinophilia of the cytoplasm were characteristic, but not limited to TSA. No evidence of ECF, but nevertheless abnormal distribution of proliferation zone was characteristic of SSA, whereas HP had neither. The presence of the ECF defines TSA in a more rigorous fashion than previous diagnostic criteria and also explains the biologic basis of exuberant protuberant growth associated with TSA and the lack of such growth in SSA. Recognition of this phenomenon may also help in exploring the genetic and molecular basis for differences between

  9. GUIDELINES FOR TRAINING SITUATION ANALYSIS (TSA). FINAL REPORT.

    ERIC Educational Resources Information Center

    CHENZOFF, ANDREW P.; FOLLEY, JOHN D., JR.

    THESE GUIDELINES REPRESENT A TEXTBOOK FOR INSTRUCTION IN THREE PHASES OF TRAINING SITUATION ANALYSIS (TSA), A STANDARDIZED PROCEDURE DEVELOPED BY THE NAVAL TRAINING DEVICE CENTER FOR SYSTEMATICALLY GATHERING AND INTERPRETING THE INFORMATION RELEVANT TO THE PLANNING OF TRAINING AND TRAINING DEVICES. THREE PHASES OF TSA ARE DESCRIBED IN…

  10. 49 CFR 1522.109 - TSA review and approval.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY SECURITY RULES FOR ALL MODES OF TRANSPORTATION TSA-APPROVED VALIDATION FIRMS AND VALIDATORS TSA-Approved Validation Firms and Validators for the Certified Cargo Screening Program... that the applicant is qualified to be a validation firm. (b) Notice.—(1) Approval. If an application is...

  11. Autotaxin is induced by TSA through HDAC3 and HDAC7 inhibition and antagonizes the TSA-induced cell apoptosis

    PubMed Central

    2011-01-01

    Background Autotaxin (ATX) is a secreted glycoprotein with the lysophospholipase D (lysoPLD) activity to convert lysophosphatidylcholine (LPC) into lysophosphatidic acid (LPA), a bioactive lysophospholipid involved in diverse biological actions. ATX is highly expressed in some cancer cells and contributes to their tumorigenesis, invasion, and metastases, while in other cancer cells ATX is silenced or expressed at low level. The mechanism of ATX expression regulation in cancer cells remains largely unknown. Results In the present study, we demonstrated that trichostatin A (TSA), a well-known HDAC inhibitor (HDACi), significantly induced ATX expression in SW480 and several other cancer cells with low or undetectable endogenous ATX expression. ATX induction could be observed when HDAC3 and HDAC7 were down-regulated by their siRNAs. It was found that HDAC7 expression levels were low in the cancer cells with high endogenous ATX expression. Exogenous over-expression of HDAC7 inhibited ATX expression in these cells in a HDAC3-dependent manner. These data indicate that HDAC3 and HDAC7 collaboratively suppress ATX expression in cancer cells, and suggest that TSA induce ATX expression by inhibiting HDAC3 and HDAC7. The biological significance of this regulation mechanism was revealed by demonstrating that TSA-induced ATX protected cancer cells against TSA-induced apoptosis by producing LPA through its lysoPLD activity, which could be reversed by BrP-LPA and S32826, the inhibitors of the ATX-LPA axis. Conclusions We have demonstrated that ATX expression is repressed by HDAC3 and HDAC7 in cancer cells. During TSA treatment, ATX is induced due to the HDAC3 and HDAC7 inhibition and functionally antagonizes the TSA-induced apoptosis. These results reveal an internal HDACi-resistant mechanism in cancer cells, and suggest that the inhibition of ATX-LPA axis would be helpful to improve the efficacy of HDACi-based therapeutics against cancer. PMID:21314984

  12. TSA - A Two Scale Approximation for Wind-Generated Ocean Surface Waves

    DTIC Science & Technology

    2012-09-30

    formulation dTSA is used. Figure 3. As in Figure 2, comparison of results from three Snl formulations, namely DIA, WRT also known as FBI, and the...double TSA formulation, dTSA , to observed 1-d data at three buoys, at the peak of hurricane Juan, 0300 UTC on 29 Sept 2003.

  13. Second-stage transsphenoidal approach (TSA) for highly vascular pituicytomas in children.

    PubMed

    Kim, Young Gyu; Park, Young Seok

    2015-06-01

    A pituicytoma in the sellar area is extremely rare in children and, due to its highly vascularized nature, can be difficult to address using the transsphenoid approach (TSA) to surgery. Here, we report a rare case of a pituicytoma that was completely removed from a child through a staged operation using the TSA. A 13-year-old girl was admitted with a 1-year history of visual disturbance and amenorrhea. Visual field examination showed left total blindness and right temporal hemianopsia. Laboratory results revealed hormonal levels all within normal ranges. Brain magnetic resonance imaging (MRI) showed a homogeneous, highly enhancing sellar and suprasellar mass, typically suggestive of a pituitary adenoma. TSA surgery revealed the tumor had a rubbery-firm consistency, hypervascularity, and profuse bleeding. We removed the tumor partially and planned a second-stage operation. Gross total removal is the treatment of choice for this type of tumor. Attempted resection of these presumed adenomas or meningiomas using the TSA often results in unexpectedly heavy intraoperative bleeding due to the high vascularity of this rare tumor, making surgery challenging, especially in children where the tumor is within a relatively narrow corridor. While pituicytomas are a rare differential diagnosis for sellar or parasellar tumors in children, total removal by second-stage TSA surgery is indicated in the case of profuse bleeding or uncertainty of biopsy. Following first-stage TSA surgery and pathologic confirmation of pituicytoma, the strategy is typically gross total removal during second-stage TSA surgery. Although very rare in children, a pituicytoma should be included in the differential diagnosis of a mass in the sellar area if the tumor is highly enhancing or very vascular. Second-stage TSA surgery is another strategy when the pathology is not clear during the first-stage TSA surgery.

  14. Aviation security : TSA has completed key activities associated with implementing secure flight, but additional actions are needed to mitigate risks.

    DOT National Transportation Integrated Search

    2009-05-01

    To enhance aviation security, the Department of Homeland Securitys (DHS) Transportation Security Administration (TSA) developed a programknown as Secure Flightto assume from air carriers the function of matching passenger information against...

  15. 77 FR 13695 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel ROYALISTE; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-07

    ... Administrative Waiver of the Coastwise Trade Laws: Vessel ROYALISTE; Invitation for Public Comments AGENCY... (MARAD), is authorized to grant waivers of the U.S.-build requirement of the coastwise laws under certain... ROYALISTE is: Intended Commercial Use of Vessel: ``Moored dockside attraction vessel and occasional sail...

  16. Synthesis and evaluation of [125I]I-TSA as a brain nicotinic acetylcholine receptor alpha7 subtype imaging agent.

    PubMed

    Ogawa, Mikako; Tatsumi, Ryo; Fujio, Masakazu; Katayama, Jiro; Magata, Yasuhiro

    2006-04-01

    Some in vitro investigations have suggested that the nicotinic acetylcholine receptor (nAChR) alpha7 subtype is implicated in Alzheimer's disease, schizophrenia and others. Recently, we developed (R)-3'-(5-bromothiophen-2-yl)spiro[1-azabicyclo[2.2.2]octane-3,5'-[1',3']oxazolidin]-2'-one (Br-TSA), which has a high affinity and selectivity for alpha7 nAChRs. Therefore we synthesized (R)-3'-(5-[125I]iodothiophen-2-yl)spiro[1-azabicyclo[2.2.2]octane-3,5'-[1',3']oxazolidin]-2'-one ([125I]I-TSA) and evaluated its potential for the in vivo detection of alpha7 nAChR in brain. In vitro binding affinity of I-TSA was measured in rat brain homogenates. Radioiodination was accomplished by a Br-I exchange reaction. Biodistribution studies were undertaken in mice by tail vein injection of [(125)I]I-TSA. In vivo receptor blocking studies were carried out by treating mice with methyllycaconitine (MLA; 5 nmol/5 mul, i.c.v.) or nonradioactive I-TSA (50 micromol/kg, i.v.). I-TSA exhibited a high affinity and selectivity for the alpha7 nAChR (K(i) for alpha7 nAChR = 0.54 nM). Initial uptake in the brain was high (4.42 %dose/g at 5 min), and the clearance of radioactivity was relatively slow in the hippocampus (alpha7 nAChR-rich region) and was rather rapid in the cerebellum (alpha7 nAChR poor region). The hippocampus to cerebellum uptake ratio was 0.9 at 5 min postinjection, but it was increased to 1.8 at 60 min postinjection. Although the effect was not statistically significant, administration of I-TSA and MLA decreased the accumulation of radioactivity in hippocampus. Despite its high affinity and selectivity, [125I]I-TSA does not appear to be a suitable tracer for in vivo alpha7 nAChR receptor imaging studies due to its high nonspecific binding. Further structural optimization is needed.

  17. Limited antigenic variation in the Trypanosoma cruzi candidate vaccine antigen TSA-1.

    PubMed

    Knight, J M; Zingales, B; Bottazzi, M E; Hotez, P; Zhan, B

    2014-12-01

    Chagas disease (American trypanosomiasis caused by Trypanosoma cruzi) is one of the most important neglected tropical diseases in the Western Hemisphere. The toxicities and limited efficacies of current antitrypanosomal drugs have prompted a search for alternative technologies such as a therapeutic vaccine comprised of T. cruzi antigens, including a recombinant antigen encoding the N-terminal 65 kDa portion of Trypomastigote surface antigen-1 (TSA-1). With at least six known genetically distinct T. cruzi lineages, variability between the different lineages poses a unique challenge for the development of broadly effective therapeutic vaccine. The variability across the major lineages in the current vaccine candidate antigen TSA-1 has not previously been addressed. To assess the variation in TSA-1, we cloned and sequenced TSA-1 from several different T. cruzi strains representing three of the most clinically relevant lineages. Analysis of the different alleles showed limited variation in TSA-1 across the different strains and fit with the current theory for the evolution of the different lineages. Additionally, minimal variation in known antigenic epitopes for the HLA-A 02 allele suggests that interlineage variation in TSA-1 would not impair the range and efficacy of a vaccine containing TSA-1. © 2014 John Wiley & Sons Ltd.

  18. 78 FR 33149 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel CATTITUDE; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-03

    ... Administrative Waiver of the Coastwise Trade Laws: Vessel CATTITUDE; Invitation for Public Comments AGENCY... Floor, Room W12-140, 1200 New Jersey Avenue SE., Washington, DC 20590. You may also send comments electronically via the Internet at http://www.regulations.gov . All comments will become part of this docket and...

  19. 76 FR 73771 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel OCEAN VUE; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-29

    ... Administrative Waiver of the Coastwise Trade Laws: Vessel OCEAN VUE; Invitation for Public Comments AGENCY... entered into this docket is available on the World Wide Web at http://www.regulations.gov . FOR FURTHER... OCEAN VUE is: Intended Commercial Use of Vessel: ``conduct water tour for vacationers & tourists...

  20. 78 FR 20171 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SEA GEANIE; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-03

    ... Administrative Waiver of the Coastwise Trade Laws: Vessel SEA GEANIE; Invitation for Public Comments AGENCY... entered into this docket is available on the World Wide Web at http://www.regulations.gov . FOR FURTHER....gov . SUPPLEMENTARY INFORMATION: As described by the applicant the intended service of the vessel SEA...

  1. 78 FR 68148 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SEA HUNT; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-13

    ... Administrative Waiver of the Coastwise Trade Laws: Vessel SEA HUNT; Invitation for Public Comments AGENCY... and all documents entered into this docket is available on the World Wide Web at http://www... of the vessel SEA HUNT is: Intended Commercial Use of Vessel: ``Charter for pleasure, overnight stays...

  2. 78 FR 64283 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SEA HAWK; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-28

    ... Administrative Waiver of the Coastwise Trade Laws: Vessel SEA HAWK; Invitation for Public Comments AGENCY... entered into this docket is available on the World Wide Web at http://www.regulations.gov . FOR FURTHER....gov . SUPPLEMENTARY INFORMATION: As described by the applicant the intended service of the vessel SEA...

  3. 77 FR 16583 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel CLOUD NINE; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-21

    ... Administrative Waiver of the Coastwise Trade Laws: Vessel CLOUD NINE; Invitation for Public Comments AGENCY... vessel CLOUD NINE is: INTENDED COMMERCIAL USE OF VESSEL: ``Local and long range sailing charters.... vessel builders or businesses in the U.S. that use U.S.-flag vessels. If MARAD determines, in accordance...

  4. 78 FR 64283 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel RAINBOW'S END; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-28

    ... Administrative Waiver of the Coastwise Trade Laws: Vessel RAINBOW'S END; Invitation for Public Comments AGENCY... and all documents entered into this docket is available on the World Wide Web at http://www... of the vessel RAINBOW'S END is: Intended Commercial Use Of Vessel: ``1 day to 2 week cruising...

  5. 77 FR 151 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SEA QUEST; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-03

    ... Administrative Waiver of the Coastwise Trade Laws: Vessel SEA QUEST; Invitation for Public Comments AGENCY... entered into this docket is available on the World Wide Web at http://www.regulations.gov . FOR FURTHER... QUEST is: Intended Commercial Use of Vessel: ``Short term charters, sport fishing and pleasure cruising...

  6. Inactivation of EGFR/AKT signaling enhances TSA-induced ovarian cancer cell differentiation.

    PubMed

    Shao, Genbao; Lai, Wensheng; Wan, Xiaolei; Xue, Jing; Wei, Ye; Jin, Jie; Zhang, Liuping; Lin, Qiong; Shao, Qixiang; Zou, Shengqiang

    2017-05-01

    Ovarian tumor is one of the most lethal gynecologic cancers, but differentiation therapy for this cancer is poorly characterized. Here, we show that thrichostatin A (TSA), the well known inhibitor of histone deacetylases (HDACs), can induce cell differentiation in HO8910 ovarian cancer cells. TSA-induced cell differentiation is characterized by typical morphological change, increased expression of the differentiation marker FOXA2, decreased expression of the pluripotency markers SOX2 and OCT4, suppressing cell proliferation, and cell cycle arrest in the G1 phase. TSA also induces an elevated expression of cell cycle inhibitory protein p21Cip1 along with a decrease in cell cycle regulatory protein cyclin D1. Significantly, blockage of epidermal growth factor receptor (EGFR) signaling pathway with specific inhibitors of this signaling cascade promotes the TSA-induced differentiation of HO8910 cells. These results imply that the EGFR cascade inhibitors in combination with TSA may represent a promising differentiation therapy strategy for ovarian cancer.

  7. 78 FR 25532 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel OCEAN JEDI; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-01

    ... Administrative Waiver of the Coastwise Trade Laws: Vessel OCEAN JEDI; Invitation for Public Comments AGENCY... entered into this docket is available on the World Wide Web at http://www.regulations.gov . FOR FURTHER... OCEAN JEDI is: Intended Commercial Use Of Vessel: ``The intended commercial use of Ocean Jedi is to...

  8. 49 CFR 1522.121 - Security threat assessments for personnel of TSA-approved validation firms.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...-approved validation firms. 1522.121 Section 1522.121 Transportation Other Regulations Relating to... FOR ALL MODES OF TRANSPORTATION TSA-APPROVED VALIDATION FIRMS AND VALIDATORS TSA-Approved Validation... for personnel of TSA-approved validation firms. Each of the following must successfully complete a...

  9. Training Systems Product Group (TSPG) Training Systems Acquisition (TSA)

    DTIC Science & Technology

    2006-05-23

    ES) 10. SPONSOR/MONITOR’S ACRONYM(S) 11. SPONSOR/MONITOR’S REPORT NUMBER(S) 12 . DISTRIBUTION/AVAILABILITY STATEMENT Approved for public release...Planned Actions • TSA III – Acquisition Strategy – Small Business • TSA II Points of Contact 3 Dominant Air Power: Design For Tomorrow…Deliver Today...Provides ready access to Large and Small Businesses Specializing in Air Force Training Systems Primarily Supporting Wright Patterson AFB OH and Hill AFB

  10. 78 FR 64284 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel BIG OL; Invitation for Public...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-28

    ... Administrative Waiver of the Coastwise Trade Laws: Vessel BIG OL; Invitation for Public Comments AGENCY: Maritime....gov . SUPPLEMENTARY INFORMATION: As described by the applicant the intended service of the vessel BIG OL is: Intended Commercial Use of Vessel: ``Limited 6 or fewer charter.'' Geographic Region: ``New...

  11. Learn, Grow, Become. TSA Edition.

    ERIC Educational Resources Information Center

    Oklahoma State Dept. of Vocational and Technical Education, Stillwater. Curriculum and Instructional Materials Center.

    This revised curriculum guide contains five units that are designed to help students develop an understanding of the mission of the Technology Student Association (TSA). Each unit follows a standard format that includes some or all of these basic components: performance objectives, suggested activities, handouts, information sheets, supplements,…

  12. 78 FR 76405 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel SALISH SEA-KR; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-17

    ... Administrative Waiver of the Coastwise Trade Laws: Vessel SALISH SEA-KR; Invitation for Public Comments AGENCY... entered into this docket is available on the World Wide Web at http://www.regulations.gov . FOR FURTHER... SALISH SEA-KR is: Intended Commercial Use Of Vessel: ``Passenger Vessel, charters'' Geographic Region...

  13. 76 FR 66127 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel CAP II; Invitation for Public...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-25

    ... Administrative Waiver of the Coastwise Trade Laws: Vessel CAP II; Invitation for Public Comments AGENCY: Maritime....gov . SUPPLEMENTARY INFORMATION: As described by the applicant the intended service of the vessel CAP II is: Intended Commercial Use of Vessel: ``CAP II is a charter vessel used for pleasure sailing only...

  14. 77 FR 66912 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel E SEA RIDER; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-07

    ... Administrative Waiver of the Coastwise Trade Laws: Vessel E SEA RIDER; Invitation for Public Comments AGENCY... entered into this docket is available on the World Wide Web at http://www.regulations.gov . FOR FURTHER... SEA RIDER is: Intended Commercial Use of Vessel: ``Short term (two to seven days) sailing charters...

  15. Inhibition of autophagy induced by TSA sensitizes colon cancer cell to radiation.

    PubMed

    He, Gang; Wang, Yan; Pang, Xueli; Zhang, Bo

    2014-02-01

    Radiotherapy is one of the main treatments for clinical cancer therapy. However, its application was limited due to lack of radiosensitivity in some cancers. Trichostatin A (TSA) is a classic histone deacetylases inhibitor (HDACi) that specifically inhibits the biochemical functions of HDAC and is demonstrated to be an active anticancer drug. However, whether it could sensitize colon cancer to radiation is not clear. Our results showed that TSA enhanced the radiosensitivity of colon cancer cells as determined by CCK-8 and clonogenic survival assay. Moreover, apoptotic cell death induced by radiation was enhanced by TSA treatment. Additionally, TSA also induced autophagic response in colon cancer cells, while autophagy inhibition led to cell apoptosis and enhanced the radiosensitivity of colon cancer cells. Our data suggested that inhibition of cytoprotective autophagy sensitizes cancer cell to radiation, which might be further investigated for clinical cancer radiotherapy.

  16. 76 FR 27655 - Intent To Request Renewal From OMB of One Current Public Collection of Information; Imposition...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-12

    ..., performed by an independent certified public accountant, of its security service fee activities and accounts...] Intent To Request Renewal From OMB of One Current Public Collection of Information; Imposition and..., DHS. ACTION: 60-day Notice. SUMMARY: The Transportation Security Administration (TSA) invites public...

  17. A metabolic screening study of trichostatin A (TSA) and TSA-like histone deacetylase inhibitors in rat and human primary hepatocyte cultures.

    PubMed

    Elaut, G; Laus, G; Alexandre, E; Richert, L; Bachellier, P; Tourwé, D; Rogiers, V; Vanhaecke, T

    2007-04-01

    Hydroxamic acid (HA)-based histone deacetylase (HDAC) inhibitors, with trichostatin A (TSA) as the reference compound, are potential antitumoral drugs and show promise in the creation of long-term primary cell cultures. However, their metabolic properties have barely been investigated. TSA is rapidly inactivated in rodents both in vitro and in vivo. We previously found that 5-(4-dimethylaminobenzoyl)aminovaleric acid hydroxyamide or 4-Me2N-BAVAH (compound 1) is metabolically more stable upon incubation with rat hepatocyte suspensions. In this study, we show that human hepatocytes also metabolize TSA more rapidly than compound 1 and that similar pathways are involved. Furthermore, structural analogs of compound 1 (compounds 2-9) are reported to have the same favorable metabolic properties. Removal of the dimethylamino substituent of compound 1 creates a very stable but 50% less potent inhibitor. Chain lengthening (4 to 5 carbon spacer) slightly improves both potency and metabolic stability, favoring HA reduction to hydrolysis. On the other hand, Calpha-unsaturation and spacer methylation not only reduce HDAC inhibition but also increase the rate of metabolic inactivation approximately 2-fold, mainly through HA reduction. However, in rat hepatocyte monolayer cultures, compound 1 is shown to be extensively metabolized by phase II conjugation. In conclusion, this study suggests that simple structural modifications of amide-linked TSA analogs can improve their phase I metabolic stability in both rat and human hepatocyte suspensions. Phase II glucuronidation, however, can compensate for their lower phase I metabolism in rat hepatocyte monolayers and could play a yet unidentified role in the determination of their in vivo clearance.

  18. Epstein-Barr virus-infected Akata cells are sensitive to histone deacetylase inhibitor TSA-provoked apoptosis.

    PubMed

    Kook, Sung-Ho; Son, Young-Ok; Han, Seong-Kyu; Lee, Hyung-Soon; Kim, Beom-Tae; Jang, Yong-Suk; Choi, Ki-Choon; Lee, Keun-Soo; Kim, So-Soon; Lim, Ji-Young; Jeon, Young-Mi; Kim, Jong-Ghee; Lee, Jeong-Chae

    2005-11-30

    Epstein-Barr virus (EBV) infects more than 90 % of the world's population and has a potential oncogenic nature. A histone deacetylase (HDAC) inhibitor, trichostatin A (TSA), has shown potential ability in cancer chemoprevention and treatment, but its effect on EBV-infected Akata cells has not been examined. This study investigated the effect of TSA on the proliferation and apoptosis of the cells. TSA inhibited cell growth and induced cytotoxicity in the EBV-infected Akata cells. TSA treatment sensitively induced apoptosis in the cell, which was demonstrated by the increased number of positively stained cells in the TUNEL assay, the migration of many cells to the sub-G0/G1 phase in flow cytometric analysis, and the ladder formation of genomic DNA. Western blot analysis showed that caspase-dependent pathways are involved in the TSA-induced apoptosis of EBV-infected Akata cells. Overall, this study shows that EBV-infected B lymphomas are quite sensitive to TSA-provoked apoptosis.

  19. Surface transportation security : TSA has taken actions to manage risk, improve coordination, and measure performance, but additional actions would enhance its efforts, April 21, 2010.

    DOT National Transportation Integrated Search

    2010-04-21

    Terrorist attacks on surface transportation facilities in Moscow, Mumbai, London, and Madrid caused casualties and highlighted the vulnerability of such systems. The Transportation Security Administration (TSA), within the Department of Homeland Secu...

  20. TSA-induced cell death in prostate cancer cell lines is caspase-2 dependent and involves the PIDDosome.

    PubMed

    Taghiyev, Agshin F; Guseva, Natalya V; Glover, Rebecca A; Rokhlin, Oskar W; Cohen, Michael B

    2006-09-01

    The histone deacetylase inhibitor Trichostatin A (TSA) has previously been found to induce caspase activity in the human prostate cancer cell lines DU145 and LNCaP. TSA treatment resulted in the release of cytochrome c and Smac/DIABLO from mitochondria in DU145, and activation of caspase-9 in both cell lines. We concluded that TSA mediated its effect via the mitochondrial pathway. The aim of the current study was to determine how TSA initiated the caspase cascade. The results revealed that caspase-2 plays an important role in TSA-induced apoptosis. Inhibition of caspase-2 by siRNA or expression of caspase-2dn substantially decreased caspase activity after TSA treatment in both cell lines, siRNA caspase-2 also inhibited TSA-induced cell death. Caspase-2 acts upstream of caspase-8 and -9 and mediates mitochondrial cytochrome c release. Coimmunoprecipitation experiments show that caspase-2 formed protein complexes with RADD/RAIDD and PIDD. Together, these data indicate that caspase-2 initiates caspase cascade after TSA treatment and involves the formation of the PIDDosome.

  1. 77 FR 4054 - Extension of Agency Information Collection Activity Under OMB Review: TSA Customer Comment Card

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-26

    ... Information Collection Activity Under OMB Review: TSA Customer Comment Card AGENCY: Transportation Security...). TSA uses a customer comment card to collect passenger comments at airports, including complaints... other forms of information technology. Information Collection Requirement Title: TSA Customer Comment...

  2. The effects of anticancer drugs TSA and GSK on spermatogenesis in male mice.

    PubMed

    Song, Wen-Yan; Yang, Qing-Ling; Zhao, Wan-Li; Jin, Hai-Xia; Yao, Gui-Dong; Peng, Zhao-Feng; Shi, Sen-Lin; Yang, Hong-Yi; Zhang, Xiang-Yang; Sun, Ying-Pu

    2016-01-01

    The effect of anticancer drugs Trichostation A (TSA) and GSK2126458 (GSK) on genetic recombination of sperm meiosis in mice was investigated, and their clinical feasibility of fertility preservation in cancer patients was also assessed. Eighteen Kunming mice were randomly given TSA or GSK at the concentrations of 0, 0.1 and 0.2 umol/L for three months. Immunofluorescence was used to evaluate the genetic recombination of homologous chromosomes and fidelity of chromosome synapsis. Sperm density, motility and viability were also examined to investigate the spermatogenic function. The average number of MLH1 foci in each spermatocyte was greatly higher in TSA (0.1) group than that in control (P<0.05), but no difference with the TSA (0.2) group (P>0.05). The frequency of SC with no MLH1 foci was lower while the frequency of SC with one MLH1 foci was higher in spermatocyte of mice with different doses of TSA compared with controls (P<0.05). The weight of left testis in TSA (0.1) group was significant decreased compared with that in control (P<0.05). However, no significant differences were observed in average number of MLH1, frequency of SC with 0-3 MLH1 foci, spermatocyte percentage of XY chromosomes containing MLH1 foci and percentages of cells containing gaps and splits among groups with or without the treatment of GSK. Furthermore, there were no statistical differences in body weight, testicular weight, sperm density, sperm motility and sperm viability among the three groups. TSA increased genetic recombination frequency of spermatocyte meiosis. GSK had no significant effect on genetic recombination frequency of spermatocyte meiosis and spermatogenic function.

  3. Effect of histone deacetylase inhibitor trichostatin A (TSA) on the microtubular system of Tetrahymena.

    PubMed

    Kovács, P; Pállinger, Eva; Csaba, G

    2007-12-01

    Histone deacetylases can also influence acetylation of tubulin. In the present experiments, after 60 min of 10 microM trichostatin (TSA) treatment the structure and amount of tubulin and acetylated-tubulin were studied immunocytochemically, by using confocal microscopy and flow cytometry. In TSA-treated Tetrahymena cells deep fibres were never labeled with antibody to acetylated tubulin. Flow cytometry with anti acetylated-tubulin antibody demonstrated that in the contol cell populations there were weaker and stronger labelled parts. After TSA treatment in the weaker labeled part the cell number decreased, and in the stronger labeled part increased significantly: this means that after the histone deacetylase inhibitor TSA treatment the amount of acetylated-tubulin in numerous Tetrahymena cells is significantly elevated. Labeling with anti-tubulin antibody was not changed significantly. On the basis of these results we postulate that histone deacetylase also in Tetrahymena influences the acetylation of tubulin, and this enzyme is sensitive to TSA treatments.

  4. Trichostatin A (TSA) sensitizes the human prostatic cancer cell line DU145 to death receptor ligands treatment.

    PubMed

    Taghiyev, Agshin F; Guseva, Natalya V; Sturm, Mary T; Rokhlin, Oskar W; Cohen, Michael B

    2005-04-01

    The human prostatic carcinoma cell line DU145 has previously been found to be resistant to treatment with TNF-family ligands. However, TRAIL, TNF-alpha and anti-Fas antibodies (Ab) treatment in combination with the histone deacetylase inhibitor Trichostatin A (TSA) converted the phenotype of DU145 from resistant to sensitive. TSA induced 15% cell death but simultaneous treatment with TRAIL, TNF-alpha and anti-Fas Ab resulted in 55%, 70% and 40% cell death, respectively. Simultaneous treatment did not increase the level of TSA-induced histone acetylation, but induced the release of acetylated histones from chromatin into the cytosol. This release was caspase dependent since it was abrogated by Z-VAD-fmk. In addition, treatment with TSA induced caspase-9 activation and resulted in the release of cytochrome c and Smac/DIABLO from mitochondria. To further investigate the role of caspase-9 in TSA-mediated apoptosis we used two different approaches: (1) cells were pretreated with the caspase-9 inhibitor Z-LEHD-fmk, and (2) cells were transfected with a dominant-negative form of caspase-9. Both approaches gave similar results: cells became resistant to treatment with TSA. These data indicate that TSA mediates its effect via the mitochondrial pathway. This was confirmed by examining DU145 overexpressing Bcl-2. These transfectants were resistant to TSA treatment. Taken together, our data shows that only simultaneous treatment with TNF-family ligands and TSA in DU145 resulted in caspase activity sufficient to induce apoptosis. The combination of TSA and TNF-family ligands could potentially be the basis for the treatment of prostate cancer.

  5. 78 FR 72922 - TSA Pre✓TM

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-04

    ... enrollment sites at airports and at off-airport locations. TSA will also explore temporary mobile enrollment at corporate offices, conferences, and other venues that choose to provide this service to their... rollout strategy. First, CBP records show that of the approximately one million annual Global Entry...

  6. 75 FR 78262 - Intent To Request Approval From OMB of One New Public Collection of Information: Airport...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-15

    ...The Transportation Security Administration (TSA) invites public comment on a new Information Collection Request (ICR) (abstracted below) that we will submit to the Office of Management and Budget (OMB) for approval in compliance with the Paperwork Reduction Act (PRA). The ICR describes the nature of the information collection and its expected burden. The collection involves the airport operator submitting a Statement of Interest (SOI) and a Federalization Request Letter (FRL) to request that TSA provide passenger and baggage screening services, that is, ``Federalization'' of an airport.

  7. The TSA Dragon--How to Slay It.

    ERIC Educational Resources Information Center

    Jeffers, Robert D.; Lamkin, Robert

    1983-01-01

    Three types of structures used in university systems to permit faculty and staff to take advantage of voluntary Tax Sheltered Annuity (TSA) programs are discussed. An innovative system at the University of Nevada is described. (MLW)

  8. HDAC inhibitor TSA ameliorates mechanical hypersensitivity and potentiates analgesic effect of morphine in a rat model of bone cancer pain by restoring μ-opioid receptor in spinal cord.

    PubMed

    Hou, Xinran; Weng, Yingqi; Ouyang, Bihan; Ding, Zhuofeng; Song, Zongbin; Zou, Wangyuan; Huang, Changsheng; Guo, Qulian

    2017-08-15

    Bone cancer pain (BCP) is a common complication with inadequate management in patients suffering from advanced cancer. Histone deacetylase inhibitors showed significant analgesic effect in multiple inflammatory and neuropathic pain models, but their effect in bone cancer pain has never been explored. In this study, we utilized a BCP rat model with intra-tibial inoculation of Walker 256 mammary gland carcinoma cells, which developed progressive mechanical hypersensitivity but not thermal hypersensitivity. Intrathecal application of trichostatin A (TSA), a classic pan-HDAC inhibitor, ameliorated tactile hypersensitivity and enhanced the analgesic effect of morphine in BCP rats. The analgesic effect of TSA was blocked by co-administration of CTAP, a specific MOR antagonist, confirming the involvement of mu-opioid receptor (MOR). A reduction of MOR expression was observed in the lumbar spinal cord of BCP rats and TSA treatment was able to partially reverse it. In vitro study in PC12 cells also demonstrated the dose-dependent enhancement of MOR expression by TSA treatment. Taking all into consideration, we could draw the conclusion that HDAC inhibitor TSA ameliorates mechanical hypersensitivity and potentiates analgesic effect of morphine in BCP rats, probably by restoring MOR expression in spinal cord. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. 49 CFR 1503.607 - Administrative law judges.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 9 2012-10-01 2012-10-01 false Administrative law judges. 1503.607 Section 1503... PROCEDURES Rules of Practice in TSA Civil Penalty Actions § 1503.607 Administrative law judges. (a) Powers of...) Administer oaths and affirmations. (4) Issue subpoenas authorized by law. (5) Rule on offers of proof. (6...

  10. 49 CFR 1503.607 - Administrative law judges.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false Administrative law judges. 1503.607 Section 1503... PROCEDURES Rules of Practice in TSA Civil Penalty Actions § 1503.607 Administrative law judges. (a) Powers of...) Administer oaths and affirmations. (4) Issue subpoenas authorized by law. (5) Rule on offers of proof. (6...

  11. Histone deacetylase inhibitors VPA and TSA induce apoptosis and autophagy in pancreatic cancer cells.

    PubMed

    Gilardini Montani, Maria Saveria; Granato, Marisa; Santoni, Claudio; Del Porto, Paola; Merendino, Nicolò; D'Orazi, Gabriella; Faggioni, Alberto; Cirone, Mara

    2017-04-01

    Histone deacetylase inhibitors (HDACi) are anti-neoplastic agents that are known to affect the growth of different cancer types, but their underlying mechanisms are still incompletely understood. Here, we compared the effects of two HDACi, i.e., Trichostatin A (TSA) and Valproic Acid (VPA), on the induction of cell death and autophagy in pancreatic cancer-derived cells that exhibit a high metastatic capacity and carry KRAS/p53 double mutations. Cell viability and proliferation tests were carried out using Trypan blue dye exclusion, MTT and BrdU assays. FACS analyses were carried out to assess cell cycle progression, apoptosis, reactive oxygen species (ROS) production and mitochondrial depolarization, while Western blot and immunoprecipitation analyses were employed to detect proteins involved in apoptosis and autophagy. We found that both VPA and TSA can induce apoptosis in Panc1 and PaCa44 pancreatic cancer-derived cells by triggering mitochondrial membrane depolarization, Cytochrome c release and Caspase 3 activation, although VPA was more effective than TSA, especially in Panc1 cells. As underlying molecular events, we found that ERK1/2 was de-phosphorylated and that the c-Myc and mutant p53 protein levels were reduced after VPA and, to a lesser extent, after TSA treatment. Up-regulation of p21 and Puma was also observed, concomitantly with mutant p53 degradation. In addition, we found that in both cell lines VPA increased the pro-apoptotic Bim level, reduced the anti-apoptotic Mcl-1 level and increased ROS production and autophagy, while TSA was able to induce these effects only in PaCA44 cells. From our results we conclude that both VPA and TSA can induce pancreatic cancer cell apoptosis and autophagy. VPA appears have a stronger and broader cytotoxic effect than TSA and, thus, may represent a better choice for anti-pancreatic cancer therapy.

  12. Performance of a proposed determinative method for p-TSA in rainbow trout fillet tissue and bridging the proposed method with a method for total chloramine-T residues in rainbow trout fillet tissue

    USGS Publications Warehouse

    Meinertz, J.R.; Stehly, G.R.; Gingerich, W.H.; Greseth, Shari L.

    2001-01-01

    Chloramine-T is an effective drug for controlling fish mortality caused by bacterial gill disease. As part of the data required for approval of chloramine-T use in aquaculture, depletion of the chloramine-T marker residue (para-toluenesulfonamide; p-TSA) from edible fillet tissue of fish must be characterized. Declaration of p-TSA as the marker residue for chloramine-T in rainbow trout was based on total residue depletion studies using a method that used time consuming and cumbersome techniques. A simple and robust method recently developed is being proposed as a determinative method for p-TSA in fish fillet tissue. The proposed determinative method was evaluated by comparing accuracy and precision data with U.S. Food and Drug Administration criteria and by bridging the method to the former method for chloramine-T residues. The method accuracy and precision fulfilled the criteria for determinative methods; accuracy was 92.6, 93.4, and 94.6% with samples fortified at 0.5X, 1X, and 2X the expected 1000 ng/g tolerance limit for p-TSA, respectively. Method precision with tissue containing incurred p-TSA at a nominal concentration of 1000 ng/g ranged from 0.80 to 8.4%. The proposed determinative method was successfully bridged with the former method. The concentrations of p-TSA developed with the proposed method were not statistically different at p < 0.05 from p-TSA concentrations developed with the former method.

  13. Synthesis, characterization and applications of a new cation exchanger tamarind sulphonic acid (TSA) resin.

    PubMed

    Singh, A V; Sharma, Naresh Kumar; Rathore, Abhay S

    2012-01-01

    A new composite cation exchanger, tamarind sulphonic acid (TSA) resin has been synthesized. The chemically modified TSA ion exchange resin has been used for the removal and preconcentration of Zn2+, Cd2+, Fe2+, Co2+ and Cu2+ ions in aqueous solution and effluent from the Laxmi steel plant in Jodhpur, India. This type of composite represents a new class of hybrid ion exchangers with good ion exchange capacity, stability, reproducibility and selectivity for toxic metal ions found in effluent from the steel industry. The characterization of the resin was carried out by determining the ion-exchange capacity, elemental analysis, pH titration, Fourier transform infrared spectra and thermal analysis. The distribution coefficients (K(d)) of toxic metal ions were determined in a reference aqueous solution and the steel plant effluent at different pH values; the absorbency of different metal ions on the TSA resin was studied for up to 10 cycles. The adsorption of different metal ions on TSA resin follows the order: Co2+ > Cu2+ > Zn2+ > Fe2+ > Cd2+. The ion exchange capacity of TSA resin is 2.87%.

  14. P53-dependent antiproliferative and pro-apoptotic effects of trichostatin A (TSA) in glioblastoma cells.

    PubMed

    Bajbouj, K; Mawrin, C; Hartig, R; Schulze-Luehrmann, J; Wilisch-Neumann, A; Roessner, A; Schneider-Stock, R

    2012-05-01

    Glioblastomas are known to be highly chemoresistant, but HDAC inhibitors (HDACi) have been shown to be of therapeutic relevance for this aggressive tumor type. We treated U87 glioblastoma cells with trichostatin A (TSA) to define potential epigenetic targets for HDACi-mediated antitumor effects. Using a cDNA array analysis covering 96 cell cycle genes, cyclin-dependent kinase inhibitor p21(WAF1) was identified as the major player in TSA-induced cell cycle arrest. TSA slightly inhibited proliferation and viability of U87 cells, cumulating in a G1/S cell cycle arrest. This effect was accompanied by a significant up-regulation of p53 and its transcriptional target p21(WAF1) and by down-regulation of key G1/S regulators, such as cdk4, cdk6, and cyclin D1. Nevertheless, TSA did not induce apoptosis in U87 cells. As expected, TSA promoted the accumulation of total acetylated histones H3 and H4 and a decrease in endogenous HDAC activity. Characterizing the chromatin modulation around the p21(WAF1) promoter after TSA treatment using chromatin immunoprecipitation, we found (1) a release of HDAC1, (2) an increase of acetylated H4 binding, and (3) enhanced recruitment of p53. p53-depleted U87 cells showed an abrogation of the G1/S arrest and re-entered the cell cycle. Immunofluorescence staining revealed that TSA induced the nuclear translocation of p21(WAF1) verifying a cell cycle arrest. On the other hand, a significant portion of p21(WAF1) was present in the cytoplasmic compartment causing apoptosis resistance. Furthermore, TSA-treated p53-mutant cell line U138 failed to show an induction in p21(WAF1), showed a deficient G2/M checkpoint, and underwent mitotic catastrophe. We suggest that HDAC inhibition in combination with other clinically used drugs may be considered an effective strategy to overcome chemoresistance in glioblastoma cells.

  15. Neuroprotective capabilities of TSA against cerebral ischemia/reperfusion injury via PI3K/Akt signaling pathway in rats.

    PubMed

    Ma, Xiao-Hui; Gao, Qiang; Jia, Zhen; Zhang, Ze-Wei

    2015-02-01

    Hundreds of previous studies demonstrated the cytoprotective effect of trichostatin-A (TSA), a kind of histone deacetylases inhibitors (HDACIs), against cerebral ischemia/reperfusion insult. Meanwhile, phosphatidylinositol-3 kinase/Akt (PI3K/Akt) is a well-known, important signaling pathway that mediates neuroprotection. However, it should be remains unclear whether the neuroprotective capabilities of TSA against cerebral ischemia/reperfusion is mediated by activation of the PI3K/Akt signaling pathway. Five groups rats (n = 12 each), with middle cerebral artery occlusion (MCAO) except sham group, were used to investigate the neuroprotective effect of certain concentration (0.05 mg/kg) of TSA, and whether the neuroprotective effect of TSA is associated with activation of the PI3K/Akt signaling pathway through using of wortmannin (0.25 mg/kg). TSA significantly increased the expression of p-Akt protein, reduced infarct volume, and attenuated neurological deficit in rats with transient MCAO, wortmannin weakened such effect of TSA dramatically. TSA could significantly decrease the neurological deficit scores and reduce the cerebral infarct volume during cerebral ischemia/reperfusion injury, which was achieved partly by activation of the PI3K/Akt signaling pathway via upgrading of p-Akt protein.

  16. [The mechanisms of p21WAF1/Cip-1 expression in MOLT-4 cell line induced by TSA].

    PubMed

    Song, Yi; Liu, Mei-Ju; Zhao, Guo-Wei; Qian, Jun-Jie; Dong, Yan; Liu, Hua; Sun, Guo-Jing; Mei, Zhu-Zhong; Liu, Bin; Tian, Bao-Lei; Sun, Zhi-Xian

    2005-04-01

    To investigate the function and molecular mechanism of p21(WAF1/Cip-1) expression in MOLT-4 cells induced by HDAC inhibitor TSA, the expression pattern of p21(WAF1/Cip-1) and the distribution of cell cycle in TSA treated cells were analyzed. The results showed that TSA could effectively induce G(2)/M arrest and apoptosis of MOLT-4 cells. Kinetic experiments demonstrated that p21(WAF1/Cip-1) were upregulated quickly before cell arrested in G(2)/M and began decreasing at the early stage of apoptosis. Meanwhile, the proteasome inhibitor MG-132 could inhibit the decrease of p21(WAF1/Cip-1) at the early stage of apoptosis, which showed that proteasome pathway involved in p21(WAF1/Cip-1) degradation during the TSA induced G(2)/M arrest and apoptosis responses. This study also identified that the protein level of p21(WAF1/Cip-1) was highly associated with the cell cycle change induced by TSA. Compared to cells treated by TSA only, exposure MOLT-4 cells to TSA meanwhile treatment with MG-132 increased the protein level of p21(WAF1/Cip-1) and increased the numbers of cell in G(2)/M-phase, whereas the cell apoptosis were delayed. It is concluded that p21(WAF1/Cip-1) plays a significant role in G(2)/M arrest and apoptosis signaling induced by TSA in MOLT-4 cells.

  17. Learn, Grow, Become. TSA Edition. Second Edition.

    ERIC Educational Resources Information Center

    Oklahoma State Dept. of Vocational and Technical Education, Stillwater. Curriculum and Instructional Materials Center.

    This curriculum guide contains seven Technology Student Association (TSA) units of study for secondary students. The units focus on helping students to develop and apply social, civic, and technology-related skills and achieve course competencies in applied technology courses. Each of the instructional units includes some or all of the basic…

  18. Breaking Boundaries and Sparking Enthusiasm with TSA

    ERIC Educational Resources Information Center

    Hess, Timothy R.

    2010-01-01

    The Technology Student Association (TSA) provides opportunities for all students who excel in technology education classes. It is really an excellent way to enrich one's classroom--utilizing the resources of an organization that promotes the importance of technological literacy. In this article, the author presents the benefits for initiating a…

  19. 49 CFR 1503.659 - Petition to reconsider or modify a final decision and order of the TSA decision maker on appeal.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... and order of the TSA decision maker on appeal. 1503.659 Section 1503.659 Transportation Other... Practice in TSA Civil Penalty Actions § 1503.659 Petition to reconsider or modify a final decision and order of the TSA decision maker on appeal. (a) General. Any party may petition the TSA decision maker to...

  20. Mapping of single-copy genes by TSA-FISH in the codling moth, Cydia pomonella.

    PubMed

    Carabajal Paladino, Leonela Z; Nguyen, Petr; Síchová, Jindra; Marec, František

    2014-01-01

    We work on the development of transgenic sexing strains in the codling moth, Cydia pomonella (Tortricidae), which would enable to produce male-only progeny for the population control of this pest using sterile insect technique (SIT). To facilitate this research, we have developed a number of cytogenetic and molecular tools, including a physical map of the codling moth Z chromosome using BAC-FISH (fluorescence in situ hybridization with bacterial artificial chromosome probes). However, chromosomal localization of unique, single-copy sequences such as a transgene cassette by conventional FISH remains challenging. In this study, we adapted a FISH protocol with tyramide signal amplification (TSA-FISH) for detection of single-copy genes in Lepidoptera. We tested the protocol with probes prepared from partial sequences of Z-linked genes in the codling moth. Using a modified TSA-FISH protocol we successfully mapped a partial sequence of the Acetylcholinesterase 1 (Ace-1) gene to the Z chromosome and confirmed thus its Z-linkage. A subsequent combination of BAC-FISH with BAC probes containing anticipated neighbouring Z-linked genes and TSA-FISH with the Ace-1 probe allowed the integration of Ace-1 in the physical map of the codling moth Z chromosome. We also developed a two-colour TSA-FISH protocol which enabled us simultaneous localization of two Z-linked genes, Ace-1 and Notch, to the expected regions of the Z chromosome. We showed that TSA-FISH represents a reliable technique for physical mapping of genes on chromosomes of moths and butterflies. Our results suggest that this technique can be combined with BAC-FISH and in the future used for physical localization of transgene cassettes on chromosomes of transgenic lines in the codling moth or other lepidopteran species. Furthermore, the developed protocol for two-colour TSA-FISH might become a powerful tool for synteny mapping in non-model organisms.

  1. Mapping of single-copy genes by TSA-FISH in the codling moth, Cydia pomonella

    PubMed Central

    2014-01-01

    Background We work on the development of transgenic sexing strains in the codling moth, Cydia pomonella (Tortricidae), which would enable to produce male-only progeny for the population control of this pest using sterile insect technique (SIT). To facilitate this research, we have developed a number of cytogenetic and molecular tools, including a physical map of the codling moth Z chromosome using BAC-FISH (fluorescence in situ hybridization with bacterial artificial chromosome probes). However, chromosomal localization of unique, single-copy sequences such as a transgene cassette by conventional FISH remains challenging. In this study, we adapted a FISH protocol with tyramide signal amplification (TSA-FISH) for detection of single-copy genes in Lepidoptera. We tested the protocol with probes prepared from partial sequences of Z-linked genes in the codling moth. Results Using a modified TSA-FISH protocol we successfully mapped a partial sequence of the Acetylcholinesterase 1 (Ace-1) gene to the Z chromosome and confirmed thus its Z-linkage. A subsequent combination of BAC-FISH with BAC probes containing anticipated neighbouring Z-linked genes and TSA-FISH with the Ace-1 probe allowed the integration of Ace-1 in the physical map of the codling moth Z chromosome. We also developed a two-colour TSA-FISH protocol which enabled us simultaneous localization of two Z-linked genes, Ace-1 and Notch, to the expected regions of the Z chromosome. Conclusions We showed that TSA-FISH represents a reliable technique for physical mapping of genes on chromosomes of moths and butterflies. Our results suggest that this technique can be combined with BAC-FISH and in the future used for physical localization of transgene cassettes on chromosomes of transgenic lines in the codling moth or other lepidopteran species. Furthermore, the developed protocol for two-colour TSA-FISH might become a powerful tool for synteny mapping in non-model organisms. PMID:25471491

  2. Fate of para-toluenesulfonamide (p-TSA) in groundwater under anoxic conditions: modelling results from a field site in Berlin (Germany).

    PubMed

    Meffe, Raffaella; Kohfahl, Claus; Hamann, Enrico; Greskowiak, Janek; Massmann, Gudrun; Dünnbier, Uwe; Pekdeger, Asaf

    2014-01-01

    This article reports on a field modelling study to investigate the processes controlling the plume evolution of para-toluenesulfonamide (p-TSA) in anoxic groundwater in Berlin, Germany. The organic contaminant p-TSA originates from the industrial production process of plasticisers, pesticides, antiseptics and drugs and is of general environmental concern for urban water management. Previous laboratory studies revealed that p-TSA is degradable under oxic conditions, whereas it appears to behave conservatively in the absence of oxygen (O2). p-TSA is ubiquitous in the aquatic environment of Berlin and present in high concentrations (up to 38 μg L(-1)) in an anoxic aquifer downgradient of a former sewage farm, where groundwater is partly used for drinking water production. To obtain refined knowledge of p-TSA transport and degradation in an aquifer at field scale, measurements of p-TSA were carried out at 11 locations (at different depths) between 2005 and 2010. Comparison of chloride (Cl(-)) and p-TSA field data showed that p-TSA has been retarded in the same manner as Cl(-). To verify the transport behaviour under field conditions, a two-dimensional transport model was setup, applying the dual-domain mass transfer approach in the model sector corresponding to an area of high aquifer heterogeneity. The distribution of Cl(-) and p-TSA concentrations from the site was reproduced well, confirming that both compounds behave conservatively and are subjected to retardation due to back diffusion from water stagnant zones. Predictive simulations showed that without any remediation measures, the groundwater quality near the drinking water well galleries will be affected by high p-TSA loads for about a hundred years.

  3. 49 CFR 1572.405 - Procedures for collection by TSA.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Collection Fee is required for a TSA agent to collect and transmit fingerprints and applicant information, in... renew an HME: $34. (3) The following FBI Fee is required for the FBI to process fingerprint...

  4. 49 CFR 1572.405 - Procedures for collection by TSA.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Collection Fee is required for a TSA agent to collect and transmit fingerprints and applicant information, in... renew an HME: $34. (3) The following FBI Fee is required for the FBI to process fingerprint...

  5. 49 CFR 1572.405 - Procedures for collection by TSA.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Collection Fee is required for a TSA agent to collect and transmit fingerprints and applicant information, in... renew an HME: $34. (3) The following FBI Fee is required for the FBI to process fingerprint...

  6. TSA increases C/EBP‑α expression by increasing its lysine acetylation in hepatic stellate cells.

    PubMed

    Tao, Li-Li; Ding, Di; Yin, Wei-Hua; Peng, Ji-Ying; Hou, Chen-Jian; Liu, Xiu-Ping; Chen, Yao-Li

    2017-11-01

    CCAAT enhancer binding protein‑α (C/EBP‑α) is a transcription factor expressed only in certain tissues, including the liver. It has been previously demonstrated that C/EBP‑α may induce apoptosis in hepatic stellate cells (HSCs), raising the question of whether acetylation of C/EBP‑α is associated with HSCs, and the potential associated mechanism. A total of three histone deacetylase inhibitors (HDACIs), including trichostatin A (TSA), suberoylanilide hydroxamic acid and nicotinamide, were selected to determine whether acetylation affects C/EBP‑α expression. A Cell Counting Kit‑8 assay was used to determine the rate of proliferation inhibition following treatment with varying doses of the three HDACIs in HSC‑T6 and BRL‑3A cells. Western blot analysis was used to examine Caspase‑3, ‑8, ‑9, and ‑12 levels in HSC‑T6 cells treated with adenoviral‑C/EBP‑α and/or TSA. Following treatment with TSA, a combination of reverse transcription‑quantitative polymerase chain reaction and western blot analyses was used to determine the inherent C/EBP‑α mRNA and protein levels in HSC‑T6 cells at 0, 1, 2, 4, 8, 12, 24, 36 and 48 h. Nuclear and cytoplasmic proteins were extracted to examine C/EBP‑α distribution. Co‑immunoprecipitation analysis was used to examine the lysine acetylation of C/EBP‑α. It was observed that TSA inhibited the proliferation of HSC‑T6 cells to a greater extent compared with BRL‑3A cells, following treatment with the three HDACIs. TSA induced apoptosis in HSC‑T6 cells and enhanced the expression of C/EBP‑α. Following treatment of HSC‑T6 cells with TSA, inherent C/EBP‑α expression increased in a time‑dependent manner, and its lysine acetylation simultaneously increased. Therefore, the results of the present study suggested that TSA may increase C/EBP‑α expression by increasing its lysine acetylation in HSCs.

  7. Suppression of IL-1beta-induced COX-2 expression by trichostatin A (TSA) in human endometrial stromal cells.

    PubMed

    Wu, Yan; Guo, Sun-Wei

    2007-11-01

    Over-production of cyclooxygenase-2 (COX-2) plays an important role in the positive feedback loop that leads to proliferation and inflammation in endometriosis. Following our observation that histone deacetylase inhibitors (HDACIs) trichostatin A (TSA) and valproic acid (VPA) can suppress proliferation of endometrial stromal cells, we sought to determine whether TSA suppresses IL-1beta-induced COX-2 expression in endometrial stromal cells. In vitro study using a recently established immortalized endometrial stromal cell line. The stromal cells were pretreated with TSA before stimulation with IL-1beta, and COX-2 gene and protein expression was measured by real-time quantitative RT-PCR and Western blot analysis, respectively. IL-1beta stimulated COX-2 expression in a concentration-dependent manner in endometrial stromal cells. The induced COX-2 gene and protein expression were suppressed by TSA pretreatment. TSA suppresses IL-1beta-induced COX-2 gene and protein expression in endometrial stromal cells. This finding, coupled with the findings that TSA and another HDACI, valproic acid, suppress proliferation and induce cell cycle arrest, suggests that HDACIs are a promising class of compound that has therapeutic potential for endometriosis.

  8. 75 FR 63191 - Intent To Request Renewal From OMB of One Current Public Collection of Information: Certified...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-14

    ...The Transportation Security Administration (TSA) invites public comment on one currently approved Information Collection Request (ICR), OMB control number 1652-0053, abstracted below that we will submit to the Office of Management and Budget (OMB) for renewal in compliance with the Paperwork Reduction Act. The ICR describes the nature of the information collection and its expected burden. The collections include: (1) Applications from entities that wish to become Certified Cargo Screening Facilities (CCSF) or operate as a TSA- approved validation firm; (2) personal information to allow TSA to conduct security threat assessments on key individuals employed by the CCSFs and validation firms; (3) implementation of a standard security program or submission of a proposed modified security program; (4) information on the amount of cargo screened; (5) recordkeeping requirements for CCSFs and validation firms; and (6) submission of validation reports to TSA. TSA is seeking the renewal of the ICR for the continuation of the program in order to secure passenger aircraft carrying cargo by the deadlines set out in the Implementing Recommendations of the 9/11 Commission Act of 2007.

  9. Aviation security : TSA is increasing procurement and deployment of the advanced imaging technology, but challenges to this effort and other areas of aviation security remain : testimony before the Subcommittee on Transportation Security and Infrastructur

    DOT National Transportation Integrated Search

    2010-03-17

    The attempted bombing of Northwest flight 253 highlighted the importance of detecting improvised explosive devices on passengers. This testimony focuses on (1) the Transportation Security Administrations (TSA) efforts to procure and deploy advance...

  10. TSA protects H9c2 cells against thapsigargin-induced apoptosis related to endoplasmic reticulum stress-mediated mitochondrial injury.

    PubMed

    Li, Zhiping; Liu, Yan; Dai, Xinlun; Zhou, Qiangqiang; Liu, Xueli; Li, Zeyu; Chen, Xia

    2017-05-01

    Endoplasmic reticulum stress (ERS) activates an adaptive unfolded protein response (UPR) that facilitates cellular repair, however, under prolonged ER stress, the UPR can ultimately trigger apoptosis thereby terminating damaged cells. Recently, TSA has shown protective effects on ERS and its mechanisms related to ER pathway has been previously characterized. However, whether TSA exerts its protective role via metabolic events remain largely undefined. Objectives : To explore the possible involvement of the metabolic changes during ERS and to better understand how TSA influence mitochondrial function to facilitate cellular adaptation. Results : TSA is an inhibitor of histone deacetylase which could significantly inhibit H9c2 cell apoptosis induced by Thapsigargin (TG). It also intervene the decrease of mitochondrial membrane potential. By immunofluorescence staining, we have shown that GRP78 was concentrated in the perinuclear region and co-localized with ER. However, treatments with TG and TSA could let it overlap with the mitochondrial marker MitoTracker. Cellular fractionation also confirmed the location of GRP78 in mitochondrion. TSA decreases ERS-induced cell apoptosis and mitochondrial injury may related to enhance the location of GRP78 in mitochondrion.

  11. DNA vaccination with a plasmid encoding LACK-TSA fusion against Leishmania major infection in BALB/c mice.

    PubMed

    Maspi, N; Ghaffarifar, F; Sharifi, Z; Dalimi, A; Khademi, S Z

    2017-12-01

    Vaccination would be the most important strategy for the prevention and elimination of leishmaniasis. The aim of the present study was to compare the immune responses induced following DNA vaccination with LACK (Leishmania analogue of the receptor kinase C), TSA (Thiol-specific-antioxidant) genes alone or LACK-TSA fusion against cutaneous leishmaniasis (CL). Cellular and humoral immune responses were evaluated before and after challenge with Leishmania major (L. major). In addition, the mean lesion size was also measured from 3th week post-infection. All immunized mice showed a partial immunity characterized by higher interferon (IFN)-γ and Immunoglobulin G (IgG2a) levels compared to control groups (p<0.05). IFN-γ/ Interleukin (IL)-4 and IgG2a/IgG1 ratios demonstrated the highest IFN-γ and IgG2a levels in the group receiving LACK-TSA fusion. Mean lesion sizes reduced significantly in all immunized mice compared with control groups at 7th week post-infection (p<0.05). In addition, there was a significant reduction in mean lesion size of LACK-TSA and TSA groups than LACK group after challenge (p<0.05). In the present study, DNA immunization promoted Th1 immune response and confirmed the previous observations on immunogenicity of LACK and TSA antigens against CL. Furthermore, this study demonstrated that a bivalent vaccine can induce stronger immune responses and protection against infectious challenge with L. major.

  12. 49 CFR 1522.115 - Renewal of TSA approval.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... operate as a validation firm. If the validation firm submits the information in the month before or after it is due, the validation firm is considered to have submitted the information in the month it is due... application. (b) Content. In addition to any other information required by TSA, the validation firm must...

  13. Creating an Effective Educational Environment for Adult Learners: A Qualitative, Multi-Case Study of Off-Campus Center Administrator's Use of Invitational Leadership

    ERIC Educational Resources Information Center

    McKnight, Carolyn P.

    2012-01-01

    This qualitative, multi-case study was designed to examine off-campus centers and their administrators in creating an effective learning environment for adult learners. Serving as the conceptual framework, invitational leadership theory is a holistic approach which nurtures the belief that everyone is intrinsically motivated and it is the leaders'…

  14. 77 FR 11146 - Intent To Request Renewal From OMB of One Current Public Collection of Information: Certified...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-24

    ...The Transportation Security Administration (TSA) invites public comment on one currently approved Information Collection Request (ICR), OMB control number 1652-0053, abstracted below that we will submit to the Office of Management and Budget (OMB) for renewal in compliance with the Paperwork Reduction Act. The ICR describes the nature of the information collection and its expected burden. The collections include: (1) Applications from entities that wish to become Certified Cargo Screening Facilities (CCSF); (2) personal information to allow TSA to conduct security threat assessments on key individuals employed by the CCSFs; (3) acceptance of a standard security program or submission of a proposed modified security program; (4) information on the amount of cargo screened; and (5) recordkeeping requirements for CCSFs. TSA is seeking the renewal of the ICR for the continuation of the program in order to secure passenger aircraft carrying cargo.

  15. The Inviting School Survey--Revised (ISS--R): A Survey for Measuring the Invitational Qualities (I.Q.) of the Total School Climate

    ERIC Educational Resources Information Center

    Smith, Kenneth H.

    2005-01-01

    This article describes the revised Inviting School Survey (ISS-R) which is a 50- item checklist based on the 100-item Inviting School Survey (Purkey & Schmidt, 1990, Purkey & Fuller, 1995). Both the original ISS and the ISS-R are designed for use by Grade Four students and above, teachers, school administrators and others associated with the…

  16. Effects of Histone Deacetylase Inhibitor (HDACi); Trichostatin-A (TSA) on the expression of housekeeping genes.

    PubMed

    Mogal, Ashish; Abdulkadir, Sarki A

    2006-04-01

    In quantitative RT-PCR (qRT-PCR), analysis of gene expression is dependent on normalization using housekeeping genes such as 18S rRNA, GAPDH and beta actin. However, variability in their expression has been reported to be caused by factors like drug treatment, pathological states and cell-cycle phase. An emerging area of cancer research focuses on identifying the role of epigenetic alterations such as histone modifications and DNA methylation in the initiation and progression of cancer. Histone acetylation is the best studied modification so far and has been probed through the use of histone deacetylase inhibitors (HDACi). Further, modulation of histone acetylation is currently being explored as a therapeutic strategy in the treatment of cancer and HDACis have shown promise in inhibiting tumorigenesis and metastasis. Trichostatin-A (TSA) is the most widely used HDACi. Therefore, we were driven to identify a suitable internal control for RT-PCR following TSA treatment. We performed quantitative RT-PCR analysis using mouse prostate tissue explants, human prostate cancer (LNCaP) cells and human breast cancer (T-47D and ZR-75-1) cells following TSA treatment. Expression of housekeeping genes including 18S rRNA, beta actin, GAPDH and ribosomal highly-basic 23-kDa protein (rb 23-kDa, RPL13A) were compared in vehicle versus TSA treated samples. Our results showed marked variations in 18S rRNA, beta actin mRNA and GAPDH mRNA levels in mouse prostate explants and a human prostate cancer (LNCaP) cell line following TSA treatment. Furthermore, in two human breast cancer cell lines (T-47D and ZR-75-1) 18S rRNA, beta actin mRNA and GAPDH mRNA levels varied significantly. However, RPL13A mRNA levels remained constant in all the conditions tested. Therefore, we recommend use of RPL13A as a standard for normalization during TSA treatment.

  17. 78 FR 44140 - Intent To Request Approval From OMB of One New Public Collection of Information: TSA PreTM

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-23

    ...-nationwide-expedited-screening-begins . TSA seeks to establish enrollment sites and implement a mobile... (or by money order at an enrollment center) to TSA's contracted vendor. Applicants then will submit...

  18. Different effects of histone deacetylase inhibitors nicotinamide and trichostatin A (TSA) in C17.2 neural stem cells.

    PubMed

    Wang, Haifeng; Cheng, Hua; Wang, Kai; Wen, Tieqiao

    2012-11-01

    Histone deacetylase inhibitors are involved in proliferation, apoptosis, cell cycle, mRNA transcription, and protein expression in various cells. However, the molecular mechanism underlying such functions is still not fully clear. In this study, we used C17.2 neural stem cell (NSC) line as a model to evaluate the effects of nicotinamide and trichostatin A (TSA) on cell characteristics. Results show that nicotinamide and TSA greatly inhibit cell growth, lead to cell morphology changes, and effectively induce cell apoptosis in a dose-dependent manner. Western blot analyses confirmed that nicotinamide significantly decreases the expression of bcl-2 and p38. Further insight into the molecular mechanisms shows the suppression of phosphorylation in eukaryotic initiation factor 4E-binding protein 1 (4EBP1) by nicotinamide, whereas, an increased expression of bcl-2 and p38 and phosphorylation of 4EBP1 by TSA. However, both nicotinamide and TSA significantly increase the expression of cytochrome c (cyt c). These results strongly suggest that bcl-2, p38, cyt c, and p-4EBP1 could suppress proliferation and induce apoptosis of C17.2 NSCs mediated by histone deacetylase inhibitors, nicotinamide and TSA, involving different molecular mechanisms.

  19. The synergistic effect of 5Azadc and TSA on maintenance of pluripotency of chicken ESCs by overexpression of NANOG gene.

    PubMed

    Wang, Xiaoyan; Wang, Yingjie; Zuo, Qisheng; Li, Dong; Zhang, Wenhui; Lian, Chao; Tang, Beibei; Xiao, Tianrong; Wang, Man; Wang, Kehua; Li, Bichun; Zhang, Yani

    2016-04-01

    NANOG is a transcription factor that functions in embryonic stem cells (ESCs) and a key factor in maintaining pluripotency. Here, we cloned the NANOG gene promoter from the Rugao yellow chicken and constructed a dual luciferase reporter vector to detect its transcriptional activity and analyze the effects of 5-aza-2'-deoxycytidine (5-Azadc) and trichostatin A (TSA) on NANOG promoter activity and ESC pluripotency maintenance in vitro. NANOG transcriptional activity was enhanced when 5-Azadc and TSA were used alone or together, suggesting the possibility of elevated methylation of the CpG island in the NANOG regulatory region. When ESCs were cultured in basic medium with 5-Azadc and TSA in vitro, significantly more cell colonies were maintained in the 5-Azadc + TSA group than in the control group, which had many differentiated cells and few cell colonies after 6 d of induction. On the tenth day of induction, the cells in the control group fully differentiated and no cell colonies remained, but many cell colonies were present in the 5-Azadc + TSA group. The expression of NANOG in the cell colonies was confirmed by indirect immunofluorescence. Furthermore, ESCs could be passaged to the 12th generation under 5-Azadc and TSA treatment and maintained their pluripotency. Thus, we showed that 5-Azadc and TSA can effectively maintain chicken ESC pluripotency in vitro by increasing NANOG gene expression.

  20. Trichostatin A (TSA) facilitates formation of partner preference in male prairie voles (Microtus ochrogaster).

    PubMed

    Duclot, F; Wang, H; Youssef, C; Liu, Y; Wang, Z; Kabbaj, M

    2016-05-01

    In the socially monogamous prairie voles (Microtus ochrogaster), the development of a social bonding is indicated by the formation of partner preference, which involves a variety of environmental and neurochemical factors and brain structures. In a most recent study in female prairie voles, we found that treatment with the histone deacetylase inhibitor trichostatin A (TSA) facilitates the formation of partner preference through up-regulation of oxytocin receptor (OTR) and vasopressin V1a receptor (V1aR) genes expression in the nucleus accumbens (NAcc). In the present study, we tested the hypothesis that TSA treatment also facilitates partner preference formation and alters OTR and V1aR genes expression in the NAcc in male prairie voles. We thus observed that central injection of TSA dose-dependently promoted the formation of partner preference in the absence of mating in male prairie voles. Interestingly, TSA treatment up-regulated OTR, but not V1aR, gene expression in the NAcc similarly as they were affected by mating - an essential process for naturally occurring partner preference. These data, together with others, not only indicate the involvement of epigenetic events but also the potential role of NAcc oxytocin in the regulation of partner preference in both male and female prairie voles. Copyright © 2016 Elsevier Inc. All rights reserved.

  1. Experimental study on inhibitory effects of histone deacetylase inhibitor MS-275 and TSA on bladder cancer cells.

    PubMed

    Qu, Wei; Kang, Yin-Dong; Zhou, Mei-Sheng; Fu, Li-Li; Hua, Zhen-Hao; Wang, Li-Ming

    2010-01-01

    To investigate the inhibitory effect of histone deacetylase (HDAC) inhibitors (MS-275 and TSA) on T24 human bladder cancer cells in vitro, and explore the possible mechanism. The MTT assay was employed to evaluate the inhibitory effect of MS-275 and TSA on T24 cell growth. FCM was used to analyze the variation of T24 cell cycle distribution and the apoptotic ratio after T24 cells were treated with MS-275 and TSA. Histone acetylation level was detected by Western blot. mRNA expression of p21 WAF1/CIP1, cyclin A, and cyclin E was measured by FQ-PCR. Dynamic changes of Bcl-2 and bax expression were detected by FCM. MS-275 and TSA inhibited T24 cell growth in a concentration and time-dependent manner. Treatment with 4 μmol/l MS-275 or 0.4 μmol/l TSA blocked cell cycling in the G0/G1 phase and induced a significant increase in cell apoptosis. MS-275 and TSA significantly increased the level of histone acetylation, induced p21CIP1WAF1 mRNA expression, and inhibited cyclin A mRNA expression, though no significant effect was observed on cyclin E. Bcl-2 expression was down-regulated, while bax expression was up-regulated. HDAC inhibitors can block bladder cancer cell cycle in vitro and induce apoptosis. The molecular mechanism may be associated with increased level of histone acetylation, down-regulation of p21WAF1/CIP1 expression, up-regulation of cyclin A expression, and dynamic change of bcl-2 and bax expression. Copyright © 2010 Elsevier Inc. All rights reserved.

  2. The influence of TSA and VPA on the in vitro differentiation of bone marrow mesenchymal stem cells into neuronal lineage cells: Gene expression studies.

    PubMed

    Fila-Danilow, Anna; Borkowska, Paulina; Paul-Samojedny, Monika; Kowalczyk, Malgorzata; Kowalski, Jan

    2017-03-27

    Epigenetic mechanisms regulate the transcription of genes, which can affect the differentiation of MSCs. The aim of the current work is to determine how the histone deacetylase inhibitors TSA and VPA affect the expression of neuronal lineage genes in a culture of rat MSCs (rMSCs). We analyzed the expression of early neuron marker gene (Tubb3), mature neuron markers genes (Vacht, Th, Htr2a) and the oligodendrocyte progenitor marker gene (GalC). Moreover, changes in the gene expression after three different periods of exposure to TSA and VPA were investigated for the first time. After six days of exposition to TSA and VPA, the expression of Tubb3 and GalC decreased, while the expression of Th increased. The highest increase of VAChT expression was observed after three days of TSA and VPA treatment. A decrease in Htr2a gene expression was observed after TSA treatment and an increase was observed after VPA treatment. We also observed that TSA and VPA inhibited cell proliferation and the formation of neurospheres in the rMSCs culture. The central findings of our study are that TSA and VPA affect the expression of neuronal lineage genes in an rMSCs culture. After exposure to TSA or VPA, the expression of early neuronal gene decreases but equally the expression of mature neuron genes increases. After TSA and VPA treatment ER of the oligodendrocyte progenitor marker decreased. TSA and VPA inhibit cell proliferation and the formation of neurospheres in rMSCs culture.

  3. Synergistic efficacy in human ovarian cancer cells by histone deacetylase inhibitor TSA and proteasome inhibitor PS-341.

    PubMed

    Fang, Yong; Hu, Yi; Wu, Peng; Wang, Beibei; Tian, Yuan; Xia, Xi; Zhang, Qinghua; Chen, Tong; Jiang, Xuefeng; Ma, Quanfu; Xu, Gang; Wang, Shixuan; Zhou, Jianfeng; Ma, Ding; Meng, Li

    2011-05-01

    Histone deacetylase inhibitors and proteasome inhibitor are all emerging as new classes of anticancer agents. We chose TSA and PS-341 to identify whether they have a synergistic efficacy on human ovarian cancer cells. After incubated with 500 nM TSA or/and 40 nM PS-341, we found that combined groups resulted in a striking increase of apoptosis and G2/M blocking rates, no matter in A2780, cisplatin-sensitive ovarian cancer cell line OV2008 or its resistant variant C13*. This demonstrated that TSA interacted synergistically with PS-341, which raised the possibility that combined the two drugs may represent a novel strategy in ovarian cancer.

  4. Cloning of a Recombinant Plasmid Encoding Thiol-Specific Antioxidant Antigen (TSA) Gene of Leishmania majorand Expression in the Chinese Hamster Ovary Cell Line.

    PubMed

    Fatemeh, Ghaffarifar; Fatemeh, Tabatabaie; Zohreh, Sharifi; Abdolhosein, Dalimiasl; Mohammad Zahir, Hassan; Mehdi, Mahdavi

    2012-01-01

    TSA (thiol-specific antioxidant antigen) is the immune-dominant antigen of Leishmania major and is considered to be the most promising candidate molecule for a recombinant or DNA vaccine against leishmaniasis. The aim of the present work was to express a plasmid containing the TSA gene in eukaryotic cells. Genomic DNA was extracted, and the TSA gene was amplified by polymerase chain reaction (PCR). The PCR product was cloned into the pTZ57R/T vector, followed by subcloning into the eukaryotic expression vector pcDNA3 (EcoRI and HindIII sites). The recombinant plasmid was characterised by restriction digest and PCR. Eukaryotic Chinese hamster ovary cells were transfected with the plasmid containing the TSA gene. Expression of the L. major TSA gene was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting. The plasmid containing the TSA gene was successfully expressed, as demonstrated by a band of 22.1 kDa on Western blots. The plasmid containing the TSA gene can be expressed in a eukaryotic cell line. Thus, the recombinant plasmid may potentially be used as a DNA vaccine in animal models.

  5. Transcription factor Sox4 is required for PUMA-mediated apoptosis induced by histone deacetylase inhibitor, TSA.

    PubMed

    Jang, Sang-Min; Kang, Eun-Jin; Kim, Jung-Woong; Kim, Chul-Hong; An, Joo-Hee; Choi, Kyung-Hee

    2013-08-23

    PUMA is a crucial regulator of apoptotic cell death mediated by p53-dependent and p53-independent mechanisms. In many cancer cells, PUMA expression is induced in response to DNA-damaging reagent in a p53-dependent manner. However, few studies have investigated transcription factors that lead to the induction of PUMA expression via p53-independent apoptotic signaling. In this study, we found that the transcription factor Sox4 increased PUMA expression in response to trichostatin A (TSA), a histone deacetylase inhibitor in the p53-null human lung cancer cell line H1299. Ectopic expression of Sox4 led to the induction of PUMA expression at the mRNA and protein levels, and TSA-mediated up-regulation of PUMA transcription was repressed by the knockdown of Sox4. Using luciferase assays and chromatin immunoprecipitation, we also determined that Sox4 recruits p300 on the PUMA promoter region and increases PUMA gene expression in response to TSA treatment. Taken together, these results suggest that Sox4 is required for p53-independent apoptotic cell death mediated by PUMA induction via TSA treatment. Crown Copyright © 2013. Published by Elsevier Inc. All rights reserved.

  6. Creating an Effective Educational Environment for Adult Learners: A Qualitative, Multi-Case Study of Off-Campus Center Administrator's Use of Invitational Leadership

    ERIC Educational Resources Information Center

    McKnight, Carolyn P.; Martin, Barbara N.

    2015-01-01

    This study was designed to examine off-campus centers and their administrators in creating an effective learning environment for adult learners using a new innovative leadership theory, invitational, which is a holistic approach that nurtures the belief everyone is intrinsically motivated and it is the leaders' responsibility to unleash their true…

  7. 49 CFR 1572.405 - Procedures for collection by TSA.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Collection Fee, Threat Assessment Fee, and FBI Fee. (a) Imposition of fees. (1) An individual who applies to... Collection Fee, Threat Assessment Fee, and FBI Fee, in a form and manner approved by TSA, when the individual... accordance with the provisions of 31 U.S.C. 9701 and other applicable Federal law. (3) The FBI Fee required...

  8. 49 CFR 1572.405 - Procedures for collection by TSA.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Collection Fee, Threat Assessment Fee, and FBI Fee. (a) Imposition of fees. (1) An individual who applies to... Collection Fee, Threat Assessment Fee, and FBI Fee, in a form and manner approved by TSA, when the individual... accordance with the provisions of 31 U.S.C. 9701 and other applicable Federal law. (3) The FBI Fee required...

  9. Prevention of Pulmonary Fibrosis via Trichostatin A (TSA) in Bleomycin Induced Rats.

    PubMed

    Ye, Qing; Li, Yanqin; Jiang, Handong; Xiong, Jianfei; Xu, Jiabo; Qin, Hui; Liu, Bin

    2014-10-20

    To investigate the effects of non selective histone deacetylase inhibitors Trichostatin A (TSA)on bleomycin-induced pulmonary fibrosis. To investigate the effects of non selective histone deacetylase inhibitors Trichostatin A ( TSA ) on HDAC2, p-SMAD2, HDAC2 mRNA, SMAD2mRNA in pulmonary fibrosis rats and investigate impossible mechanism. 46 SPF level male SD rats were randomly divided into four groups: ten for normal control group, fourteen for model control group I, twelve for model control group II and ten for treatment group. Rat pulmonary fibrosis was induced by bleomycin(5mg/kg) via single intratracheal perfusion in the two model control groups and treatment group. Normal control mice were instilled with a corresponding volume of 0.9% saline intratracheally. Treatment group was treated by the dilution of TSA 2mg/kg DMSO 60ul and0.9% saline 1.2ml intraperitoneal injection from the next day ,once a day for three days. Model control group II was treated by the dilution of DMSO 60ul and0.9% saline 1.2ml intraperitoneal injection from the next day once a day for three days. Model control group I and normal control group were treated by 0.9% saline 1.2ml intraperitoneal injection from the next day once a day for three days. All the animals were sacrificed on the 21 day after modeling. The pathological changes were observed by hematoxylin and eosin(HE)stain and masson trichrome stain. The expression of HDAC2 mRNA,SMAD2 mRNA were measured by real-time PCR. The protein level of HDAC2 and p-SMAD2 in serum was measured by Western blot. The pulmonary fibrosis in treatment group were significantly alleviated compared to the two model control groups (P<0.05). Real-time PCR showed that the treatment group had lower expression of lung tissue HDAC2 mRNA than the two model control groups and normal control group (P<0.05). The expression of lung tissue SMAD2 mRNA increased in the two model control groups and treatment group (P<0.05),but there were no significant differences

  10. Sp1 is a transcription repressor to stanniocalcin-1 expression in TSA-treated human colon cancer cells, HT29.

    PubMed

    Law, Alice Y S; Yeung, B H Y; Ching, L Y; Wong, Chris K C

    2011-08-01

    Our previous study demonstrated that, stanniocalcin-1 (STC1) was a target of histone deacetylase (HDAC) inhibitors and was involved in trichostatin A (TSA) induced apoptosis in the human colon cancer cells, HT29. In this study, we reported that the transcriptional factor, specificity protein 1 (Sp1) in association with retinoblastoma (Rb) repressed STC1 gene transcription in TSA-treated HT29 cells. Our data demonstrated that, a co-treatment of the cells with TSA and Sp1 inhibitor, mithramycin A (MTM) led to a marked synergistic induction of STC1 transcript levels, STC1 promoter (1 kb)-driven luciferase activity and an increase of apoptotic cell population. The knockdown of Sp1 gene expression in TSA treated cells, revealed the repressor role of Sp1 in STC1 transcription. Using a protein phosphatase inhibitor okadaic acid (OKA), an increase of Sp1 hyperphosphorylation and so a reduction of its transcriptional activity, led to a significant induction of STC1 gene expression. Chromatin immunoprecipitation (ChIP) assay revealed that Sp1 binding on STC1 proximal promoter in TSA treated cells. The binding of Sp1 to STC1 promoter was abolished by the co-treatment of MTM or OKA in TSA-treated cells. Re-ChIP assay illustrated that Sp1-mediated inhibition of STC1 transcription was associated with the recruitment of another repressor molecule, Rb. Collectively our findings identify STC1 is a downstream target of Sp1. Copyright © 2011 Wiley-Liss, Inc.

  11. Mitigating Localized Corrosion Using Thermally Sprayed Aluminum (TSA) Coatings on Welded 25% Cr Superduplex Stainless Steel

    NASA Astrophysics Data System (ADS)

    Paul, S.; Lu, Q.; Harvey, M. D. F.

    2015-04-01

    Thermally sprayed aluminum (TSA) coating has been increasingly used for the protection of carbon steel offshore structures, topside equipment, and flowlines/pipelines exposed to both marine atmospheres and seawater immersion conditions. In this paper, the effectiveness of TSA coatings in preventing localized corrosion, such as pitting and crevice corrosion of 25% Cr superduplex stainless steel (SDSS) in subsea applications, has been investigated. Welded 25% Cr SDSS (coated and uncoated) with and without defects, and surfaces coated with epoxy paint were also examined. Pitting and crevice corrosion tests, on welded 25% Cr SDSS specimens with and without TSA/epoxy coatings, were conducted in recirculated, aerated, and synthetic seawater at 90 °C for 90 days. The tests were carried out at both the free corrosion potentials and an applied cathodic potential of -1100 mV saturated calomel electrode. The acidity (pH) of the test solution was monitored daily and adjusted to between pH 7.5 and 8.1, using dilute HCl solution or dilute NaOH, depending on the pH of the solution measured during the test. The test results demonstrated that TSA prevented pitting and crevice corrosion of 25% Cr SDSS in artificial seawater at 90 °C, even when 10-mm-diameter coating defect exposing the underlying steel was present.

  12. Visualization of mcr mRNA in a methanogen by fluorescence in situ hybridization with an oligonucleotide probe and two-pass tyramide signal amplification (two-pass TSA-FISH).

    PubMed

    Kubota, Kengo; Ohashi, Akiyoshi; Imachi, Hiroyuki; Harada, Hideki

    2006-09-01

    Two-pass tyramide signal amplification-fluorescence in situ hybridization (two-pass TSA-FISH) with a horseradish peroxidase (HRP)-labeled oligonucleotide probe was applied to detect prokaryotic mRNA. In this study, mRNA of a key enzyme for methanogenesis, methyl coenzyme M reductase (mcr), in Methanococcus vannielii was targeted. Applicability of mRNA-targeted probes to in situ hybridization was verified by Clone-FISH. It was observed that sensitivity of two-pass TSA-FISH was significantly higher than that of TSA-FISH, which was further increased by the addition of dextran sulphate in TSA working solution. Signals from two-pass TSA-FISH were more reliable compared to the weak, spotty signals yielded by TSA-FISH.

  13. [HDAC1 expression and effect of TSA on proliferation and apoptosis of A549 cells].

    PubMed

    Huang, Hong; Zhang, Zhen-Xiang; Xu, Yong-Jian; Shao, Jing-Fang

    2003-09-01

    Histone deacetylase (HDAC) shows a high expression in many cancer cells and the inhibitor of HDAC1, trichostatin A (TSA), can inhibit the growth of cancer cells. Hypoxia is a common feature of malignant tumors. This paper was designed to investigate the expression of HDAC1 of A549 cell strains in hypoxia condition and the effect of TSA on their proliferation and apoptosis. The authors designed 1 normoxia group (control group) and 5 hypoxia groups (test groups): hypoxia 6h group (A), TSA + hypoxia 6h (B), hypoxia 12h group (C), hypoxia 24h group (D), TSA + hypoxia 24h (E), hypoxia 48h group (F). The expression of HDAC1 in A549 cells was examined using Western blot analysis. Proliferation, the apoptotic rates of A549 cells and the effect of TSA on them were determined using MTT method, immunohistochemistry, TUNEL method, and flow cytometry. The expression of mRNA of HDAC1 and the effect of TSA on it were determined using reverse transcription-polymerase chain reaction (RT-PCR). The A values expressed by HDAC1 in A549 cell strains were 138+/-11 in the control group, 78+/-4, 86+/-5, 124+/-3, and 120+/-9 in test groups A, C, D, and F, respectively. The A values of HDAC1mRNA versus the A values of beta-Atin mRNA were 0.68+/-0.03 in the control group, 0.46+/-0.03, 0.45+/-0.02, 0.70+/-0.03, and 0.33+/-0.02 in test groups A, C, D, and F, respectively. The A values of the expression of PCNA in A549 cell strains were 0.13+/-0.03 in the control group, 0.10+/-0.02, 0.11+/-0.02, 0.16+/-0.02, and 0.11+/-0.03 in test groups A, B, D, and E, respectively. The A values of MTT in A549 cell strains were 0.50+/-0.06 in the control group, 0.41+/-0.04, 0.45+/-0.03, 0.59+/-0.02, and 0.45+/-0.03 in test groups A, B, D, and E, respectively. The A values of positive cells of apoptosis in A549 cell strains were 0.16+/-0.04 in the control group, 0.18+/-0.02, 0.18+/-0.05, 0.20+/-0.05, and 0.23+/-0.05 in test groups A, B, D, and E, respectively. The apoptotic rates in A549 cells were 1.11% in the

  14. Cloning and characterization of indole synthase (INS) and a putative tryptophan synthase α-subunit (TSA) genes from Polygonum tinctorium.

    PubMed

    Jin, Zhehao; Kim, Jin-Hee; Park, Sang Un; Kim, Soo-Un

    2016-12-01

    Two cDNAs for indole-3-glycerol phosphate lyase homolog were cloned from Polygonum tinctorium. One encoded cytosolic indole synthase possibly in indigoid synthesis, whereas the other encoded a putative tryptophan synthase α-subunit. Indigo is an old natural blue dye produced by plants such as Polygonum tinctorium. Key step in plant indigoid biosynthesis is production of indole by indole-3-glycerol phosphate lyase (IGL). Two tryptophan synthase α-subunit (TSA) homologs, PtIGL-short and -long, were isolated by RACE PCR from P. tinctorium. The genome of the plant contained two genes coding for IGL. The short and the long forms, respectively, encoded 273 and 316 amino acid residue-long proteins. The short form complemented E. coli ΔtnaA ΔtrpA mutant on tryptophan-depleted agar plate signifying production of free indole, and thus was named indole synthase gene (PtINS). The long form, either intact or without the transit peptide sequence, did not complement the mutant and was tentatively named PtTSA. PtTSA was delivered into chloroplast as predicted by 42-residue-long targeting sequence, whereas PtINS was localized in cytosol. Genomic structure analysis suggested that a TSA duplicate acquired splicing sites during the course of evolution toward PtINS so that the targeting sequence-containing pre-mRNA segment was deleted as an intron. PtINS had about two to fivefolds higher transcript level than that of PtTSA, and treatment of 2,1,3-benzothiadiazole caused the relative transcript level of PtINS over PtTSA was significantly enhanced in the plant. The results indicate participation of PtINS in indigoid production.

  15. Differential effects of trichostatin A on gelatinase A expression in 3T3 fibroblasts and HT-1080 fibrosarcoma cells: implications for use of TSA in cancer therapy.

    PubMed

    Ailenberg, Menachem; Silverman, Mel

    2003-03-07

    Trichostatin A (TSA) is a histone deacetylase (HDAC) inhibitor with potential in cancer therapeutics. In a recent communication, we demonstrated that TSA is a selective, potent inhibitor of gelatinase A in 3T3 fibroblasts. In the present study, we extend these observations and examine the effects of TSA in 3T3 fibroblasts compared to HT-1080 fibrosarcoma cells with respect to gelatinase A expression, cell viability, and apoptosis. We find that while expression of gelatinase A in 3T3 fibroblasts is exquisitely sensitive to inhibition by TSA, expression of this enzyme in HT-1080 cells is minimally affected by this compound. Moreover, we show that TSA is pro-apoptotic in HT-1080 cells, but is anti-apoptotic in 3T3 cells. We propose a two-pronged model for the therapeutic action of TSA. On the one hand TSA selectively decreases cancer cell viability, while enhancing the viability of stromal cells. On the other hand, by selectively decreasing gelatinase A expression in stromal but not cancer cells, TSA acts to control metastatic potential by reducing the ability of metastatic cells to recruit stromal cells to secrete gelatinase A.

  16. Ab initio study of the binding of Trichostatin A (TSA) in the active site of histone deacetylase like protein (HDLP).

    PubMed

    Vanommeslaeghe, Kenno; Van Alsenoy, Christian; De Proft, Frank; Martins, José C; Tourwé, Dirk; Geerlings, Paul

    2003-08-21

    Histone deacetylase (HDAC) inhibitors have recently attracted considerable interest because of their therapeutic potential for the treatment of cell proliferative diseases. An X-ray structure of a very potent inhibitor, Trichostatin A (TSA), bound to HDLP (an HDAC analogue isolated from Aquifex aeolicus), is available. From this structure, an active site model (322 atoms), relevant for the binding of TSA and structural analogues, has been derived, and TSA has been minimized in this active site at HF 3-21G* level. The resulting conformation is in excellent accordance with the X-ray structure, and indicates a deprotonation of the hydroxamic acid in TSA by His 131. Also, a water molecule was minimized in the active site. In addition to a similar deprotonation, in accordance with a possible catalytic mechanism of HDAC as proposed by Finnin et al. (M. S. Finnin, J. R. Donigian, A. Cohen, V. M. Richon, R. A. Rifkind and P. A. Marks, Nature, 1999, 401, 188-193), a displacement of the resulting OH- ion in the active site was observed. Based on these results, the difference in energy of binding between TSA and water was calculated. The resulting value is realistic in respect to experimental binding affinities. Furthermore, the mechanism of action of the His 131-Asp 166 charge relay system was investigated. Although the Asp residue in this motif is known to substantially increase the basicity of the His residue, no proton transfer from His 131 to Asp 166 was observed on binding of TSA or water. However, in the empty protonated active site, this proton transfer does occur.

  17. Retinoic acids and trichostatin A (TSA), a histone deacetylase inhibitor, induce human pyruvate dehydrogenase kinase 4 (PDK4) gene expression.

    PubMed

    Kwon, Hye-Sook; Huang, Boli; Ho Jeoung, Nam; Wu, Pengfei; Steussy, Calvin N; Harris, Robert A

    2006-01-01

    Induction of pyruvate dehydrogenase kinase 4 (PDK4) conserves glucose and substrates for gluconeogenesis and thereby helps regulate blood glucose levels during starvation. We report here that retinoic acids (RA) as well as Trichostatin A (TSA), an inhibitor of histone deacetylase (HDAC), regulate PDK4 gene expression. Two retinoic acid response elements (RAREs) to which retinoid X receptor alpha (RXRalpha) and retinoic acid receptor alpha (RARalpha) bind and activate transcription are present in the human PDK4 (hPDK4) proximal promoter. Sp1 and CCAAT box binding factor (CBF) bind to the region between two RAREs. Mutation of either the Sp1 or the CBF site significantly decreases basal expression, transactivation by RXRalpha/RARalpha/RA, and the ability of TSA to stimulate hPDK4 gene transcription. By the chromatin immunoprecipitation assay, RA and TSA increase acetylation of histones bound to the proximal promoter as well as occupancy of CBP and Sp1. Interaction of p300/CBP with E1A completely prevented hPDK4 gene activation by RXRalpha/RARalpha/RA and TSA. The p300/CBP may enhance acetylation of histones bound to the hPDK4 promoter and cooperate with Sp1 and CBF to stimulate transcription of the hPDK4 gene in response to RA and TSA.

  18. TSA-induced DNMT1 down-regulation represses hTERT expression via recruiting CTCF into demethylated core promoter region of hTERT in HCT116.

    PubMed

    Choi, Jee-Hye; Min, Na Young; Park, Jina; Kim, Jin Hong; Park, Soo Hyun; Ko, Young Jong; Kang, Yoonsung; Moon, Young Joon; Rhee, Sangmyung; Ham, Seung Wook; Park, Ae Ja; Lee, Kwang-Ho

    2010-01-01

    Trichostatin A (TSA), an inhibitor of histone deacetylase, is a well-known antitumor agent that effectively and selectively induces tumor growth arrest and apoptosis. Recently, it was reported that hTERT is one of the primary targets for TSA-induced apoptosis in cancer cells but the mechanism of which has not yet been elucidated. In the present study, to better understand the epigenetic regulation mechanism responsible for the repression of hTERT by TSA, we examined expression of hTERT in the HCT116 colon cancer cell line after treatment with TSA and performed site-specific CpG methylation analysis of the hTERT promoter. We found that TSA-induced the demethylation of site-specific CpGs on the promoter of hTERT, which was caused by down-regulation of DNA methyltransferase 1 (DNMT1). Among the demethylated region, the 31st-33rd CpGs contained a binding site for CTCF, an inhibitor of hTERT transcription. ChIP analysis revealed that TSA-induced demethylation of the 31st-33rd CpGs promoted CTCF binding on hTERT promoter, leading to repression of hTERT. Taken together, down-regulation of DNMT1 by TSA caused demethylation of a CTCF binding site on the hTERT promoter, the result of which was repression of hTERT via recruitment of CTCF to the promoter. Copyright 2009 Elsevier Inc. All rights reserved.

  19. Effects of alcohol on histone deacetylase 2 (HDAC2) and the neuroprotective role of trichostatin A (TSA).

    PubMed

    Agudelo, Marisela; Gandhi, Nimisha; Saiyed, Zainulabedin; Pichili, Vijaya; Thangavel, Samikkannu; Khatavkar, Pradnya; Yndart-Arias, Adriana; Nair, Madhavan

    2011-08-01

    Previous studies have implicated histone deacetylases (HDACs) and HDAC inhibitors (HDIs) such as trichostatin A (TSA) in the regulation of gene expression during drug addiction. Furthermore, an increase in HDAC activity has been linked to neurodegeneration. Alcohol has also been shown to promote abundant generation of reactive oxygen species (ROS) resulting in oxidative stress. TSA inhibits HDACs and has been shown to be neuroprotective in other neurodegenerative disease models. Although HDACs and HDIs have been associated with drug addiction, there is no evidence of the neurodegenerative role of HDAC2 and neuroprotective role of TSA in alcohol addiction. Therefore, we hypothesize that alcohol modulates HDAC2 through mechanisms involving oxidative stress. To test our hypothesis, the human neuronal cell line, SK-N-MC, was treated with different concentrations of ethanol (EtOH); HDAC2 gene and protein expression were assessed at different time points. Pharmacological inhibition of HDAC2 with TSA was evaluated at the gene level using qRT-PCR and at the protein level using Western blot and flow cytometry. ROS production was measured with a fluorescence microplate reader and fluorescence microscopy. Our results showed a dose-dependent increase in HDAC2 expression with EtOH treatment. Additionally, alcohol significantly induced ROS, and pharmacological inhibition of HDAC2 with TSA was shown to be neuroprotective by significantly inhibiting HDAC2 and ROS. These results suggest that EtOH can upregulate HDAC2 through mechanisms involving oxidative stress and HDACs may play an important role in alcohol use disorders (AUDs). Moreover, the use of HDIs may be of therapeutic significance for the treatment of neurodegenerative disorders including AUDs. Copyright © 2011 by the Research Society on Alcoholism.

  20. Trichostatin A (TSA) improves the development of rabbit-rabbit intraspecies cloned embryos, but not rabbit-human interspecies cloned embryos.

    PubMed

    Shi, Li-Hong; Miao, Yi-Liang; Ouyang, Ying-Chun; Huang, Jun-Cheng; Lei, Zi-Li; Yang, Ji-Wen; Han, Zhi-Ming; Song, Xiang-Fen; Sun, Qing-Yuan; Chen, Da-Yuan

    2008-03-01

    The interspecies somatic cell nuclear transfer (iSCNT) technique for therapeutic cloning gives great promise for treatment of many human diseases. However, the incomplete nuclear reprogramming and the low blastocyst rate of iSCNT are still big problems. Herein, we observed the effect of TSA on the development of rabbit-rabbit intraspecies and rabbit-human interspecies cloned embryos. After treatment with TSA for 6 hr during activation, we found that the blastocyst rate of rabbit-rabbit cloned embryos was more than two times higher than that of untreated embryos; however, the blastocyst rate of TSA-treated rabbit-human interspecies cloned embryos decreased. We also found evident time-dependent histone deacetylation-reacetylation changes in rabbit-rabbit cloned embryos, but not in rabbit-human cloned embryos from fusion to 6 hr after activation. Our results suggest that TSA-treatment does not improve blastocyst development of rabbit-human iSCNT embryos and that abnormal histone deacetylation-reacetylation changes in iSCNT embryos may account for their poor blastocyst development. (c) 2008 Wiley-Liss, Inc.

  1. Synergistic effect of p53 on TSA-induced stanniocalcin 1 expression in human nasopharyngeal carcinoma cells, CNE2.

    PubMed

    Ching, L Y; Yeung, Bonnie H Y; Wong, Chris K C

    2012-06-01

    Human stanniocalcin 1 (STC1) has recently been identified as a putative protein factor involved in cellular apoptosis. The use of histone deacetylase inhibitor (i.e. trichostatin A (TSA)) and doxorubicin (Dox) is one of the common treatment methods to induce apoptosis in human cancer cells. A study on TSA and Dox-mediated apoptosis may shed light on the regulation and function of STC1 in cancer treatment. In this study, TSA and Dox cotreatment in human nasopharyngeal carcinoma cells (CNE2) elicited synergistic effects on STC1 gene expression and cellular apoptosis. An activation of p53 (TP53) transcriptional activity in Dox- or Dox+TSA-treated cells was revealed by the increased expression levels of p53 mRNA/protein as well as p53-driven luciferase activities. To elucidate the possible involvement of p53 in STC1 gene transcription, a vector expressing wild-type or dominant negative (DN) p53 was transiently transfected into the cells. Both STC1 promoter luciferase constructs and chromatin immunoprecipitation assays did not support the direct role of p53 in STC1 gene transactivation. However, the synergistic effects of p53 on the induction of NF-κB phosphorylation and the recruitment of acetylated histone H3 in STC1 promoter were observed in TSA-cotreated cells. The overexpression of exogenous STC1 sensitized apoptosis in Dox-treated cells. Taken together, this study provides data to show the cross talk of NF-κB, p53, and histone protein in the regulation of STC1 expression and function.

  2. Immunogenicity and efficacy of a bivalent DNA vaccine containing LeIF and TSA genes against murine cutaneous leishmaniasis.

    PubMed

    Maspi, Nahid; Ghaffarifar, Fatemeh; Sharifi, Zohreh; Dalimi, Abdolhossein; Dayer, Mohammad Saaid

    2017-03-01

    There is no effective vaccine for the prevention and elimination of leishmaniasis. For this reason, we assessed the protective effects of DNA vaccines containing LeIF, TSA genes alone, or LeIF-TSA fusion against cutaneous leishmaniasis pEGFP-N1 plasmid (empty vector) and phosphate buffer saline (PBS) were used as control groups. Therefore, cellular and humoral immune responses were evaluated before and after the challenge with Leishmania major. Lesion diameter was also measured 3-12 weeks after challenge. All immunized mice with plasmid DNA encoding Leishmania antigens induced the partial immunity characterized by increased IFN-γ and IgG2a levels compared with control groups (p < 0.001). Furthermore, the immunized mice showed significant reduction in mean lesion sizes compared with mice in empty vector and PBS groups (p < 0.05). The reduction in lesion diameter was 29.3%, 34.1%, and 46.2% less in groups vaccinated with LeIF, TSA, and LeIF-TSA, respectively, than in PBS group at 12th week post infection. IFN/IL-4 and IgG2a/IgG1 ratios indicated that group receiving LeIF-TSA fusion had the highest IFN-γ and IgG2a levels. In this study, DNA immunization promoted Th1 immune response characterized by higher IFN-γ and IgG2a levels and also reduction in lesion size. These results showed that a bivalent vaccine containing two distinct antigens may induce more potent immune responses against leishmaniasis. © 2017 APMIS. Published by John Wiley & Sons Ltd.

  3. Antigenic switching of TSA 417, a trophozoite variable surface protein, following completion of the life cycle of Giardia lamblia.

    PubMed Central

    Meng, T C; Hetsko, M L; Gillin, F D

    1993-01-01

    Expression of TSA 417, the predominant cysteine-rich variable surface protein of Giardia lamblia WB clone C6 trophozoites, did not change during encystation in vitro. However, in vitro excystation of cysts derived in vitro or in vivo consistently produced TSA 417 nonexpressing trophozoite populations, suggesting that completion of the life cycle leads to antigenic switching. Images PMID:8225614

  4. Protein-ligand interactions investigated by thermal shift assays (TSA) and dual polarization interferometry (DPI).

    PubMed

    Grøftehauge, Morten K; Hajizadeh, Nelly R; Swann, Marcus J; Pohl, Ehmke

    2015-01-01

    Over the last decades, a wide range of biophysical techniques investigating protein-ligand interactions have become indispensable tools to complement high-resolution crystal structure determinations. Current approaches in solution range from high-throughput-capable methods such as thermal shift assays (TSA) to highly accurate techniques including microscale thermophoresis (MST) and isothermal titration calorimetry (ITC) that can provide a full thermodynamic description of binding events. Surface-based methods such as surface plasmon resonance (SPR) and dual polarization interferometry (DPI) allow real-time measurements and can provide kinetic parameters as well as binding constants. DPI provides additional spatial information about the binding event. Here, an account is presented of new developments and recent applications of TSA and DPI connected to crystallography.

  5. A Trichostatin A (TSA)/Sp1-mediated mechanism for the regulation of SALL2 tumor suppressor in Jurkat T cells.

    PubMed

    Hepp, Matías I; Escobar, David; Farkas, Carlos; Hermosilla, Viviana; Álvarez, Claudia; Amigo, Roberto; Gutiérrez, José L; Castro, Ariel F; Pincheira, Roxana

    2018-05-17

    SALL2 is a transcription factor involved in development and disease. Deregulation of SALL2 has been associated with cancer, suggesting that it plays a role in the disease. However, how SALL2 is regulated and why is deregulated in cancer remain poorly understood. We previously showed that the p53 tumor suppressor represses SALL2 under acute genotoxic stress. Here, we investigated the effect of Histone Deacetylase Inhibitor (HDACi) Trichostatin A (TSA), and involvement of Sp1 on expression and function of SALL2 in Jurkat T cells. We show that SALL2 mRNA and protein levels were enhanced under TSA treatment. Both, TSA and ectopic expression of Sp1 transactivated the SALL2 P2 promoter. This transactivation effect was blocked by the Sp1-binding inhibitor mithramycin A. Sp1 bound in vitro and in vivo to the proximal region of the P2 promoter. TSA induced Sp1 binding to the P2 promoter, which correlated with dynamic changes on H4 acetylation and concomitant recruitment of p300 or HDAC1 in a mutually exclusive manner. Our results suggest that TSA-induced Sp1-Lys703 acetylation contributes to the transcriptional activation of the P2 promoter. Finally, using a CRISPR/Cas9 SALL2-KO Jurkat-T cell model and gain of function experiments, we demonstrated that SALL2 upregulation is required for TSA-mediated cell death. Thus, our study identified Sp1 as a novel transcriptional regulator of SALL2, and proposes a novel epigenetic mechanism for SALL2 regulation in Jurkat-T cells. Altogether, our data support SALL2 function as a tumor suppressor, and SALL2 involvement in cell death response to HDACi. Copyright © 2018. Published by Elsevier B.V.

  6. 76 FR 49503 - Intent To Request Renewal From OMB of One Current Public Collection of Information: Airport Security

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-10

    ...The Transportation Security Administration (TSA) invites public comment on one currently approved Information Collection Request (ICR), Office of Management and Budget (OMB) control number 1652-0002, abstracted below that we will submit to OMB for renewal in compliance with the Paperwork Reduction Act (PRA). The ICR describes the nature of the information collection and its expected burden. These programs require airport operators to maintain and update records to ensure compliance with security provisions outlined in 49 CFR part 1542.

  7. TSA and BIX-01294 Induced Normal DNA and Histone Methylation and Increased Protein Expression in Porcine Somatic Cell Nuclear Transfer Embryos

    PubMed Central

    Ding, Biao; Zuo, Xiaoyuan; Li, Hui; Ding, Jianping; Li, Yunsheng; Huang, Weiping; Zhang, Yunhai

    2017-01-01

    The poor efficiency of animal cloning is mainly attributed to the defects in epigenetic reprogramming of donor cells’ chromatins during early embryonic development. Previous studies indicated that inhibition of histone deacetylases or methyltransferase, such as G9A, using Trichostatin A (TSA) or BIX-01294 significantly enhanced the developmental efficiency of porcine somatic cell nuclear transfer (SCNT) embryos. However, potential mechanisms underlying the improved early developmental competence of SCNT embryos exposed to TSA and BIX-01294 are largely unclear. Here we found that 50 nM TSA or 1.0 μM BIX-01294 treatment alone for 24 h significantly elevated the blastocyst rate (P < 0.05), while further improvement was not observed under combined treatment condition. Furthermore, co-treatment or TSA treatment alone significantly reduced H3K9me2 level at the 4-cell stage, which is comparable with that in in vivo and in vitro fertilized counterparts. However, only co-treatment significantly decreased the levels of 5mC and H3K9me2 in trophectoderm lineage and subsequently increased the expression of OCT4 and CDX2 associated with ICM and TE lineage differentiation. Altogether, these results demonstrate that co-treatment of TSA and BIX-01294 enhances the early developmental competence of porcine SCNT embryos via improvements in epigenetic status and protein expression. PMID:28114389

  8. TSA and BIX-01294 Induced Normal DNA and Histone Methylation and Increased Protein Expression in Porcine Somatic Cell Nuclear Transfer Embryos.

    PubMed

    Cao, Zubing; Hong, Renyun; Ding, Biao; Zuo, Xiaoyuan; Li, Hui; Ding, Jianping; Li, Yunsheng; Huang, Weiping; Zhang, Yunhai

    2017-01-01

    The poor efficiency of animal cloning is mainly attributed to the defects in epigenetic reprogramming of donor cells' chromatins during early embryonic development. Previous studies indicated that inhibition of histone deacetylases or methyltransferase, such as G9A, using Trichostatin A (TSA) or BIX-01294 significantly enhanced the developmental efficiency of porcine somatic cell nuclear transfer (SCNT) embryos. However, potential mechanisms underlying the improved early developmental competence of SCNT embryos exposed to TSA and BIX-01294 are largely unclear. Here we found that 50 nM TSA or 1.0 μM BIX-01294 treatment alone for 24 h significantly elevated the blastocyst rate (P < 0.05), while further improvement was not observed under combined treatment condition. Furthermore, co-treatment or TSA treatment alone significantly reduced H3K9me2 level at the 4-cell stage, which is comparable with that in in vivo and in vitro fertilized counterparts. However, only co-treatment significantly decreased the levels of 5mC and H3K9me2 in trophectoderm lineage and subsequently increased the expression of OCT4 and CDX2 associated with ICM and TE lineage differentiation. Altogether, these results demonstrate that co-treatment of TSA and BIX-01294 enhances the early developmental competence of porcine SCNT embryos via improvements in epigenetic status and protein expression.

  9. Protein–ligand interactions investigated by thermal shift assays (TSA) and dual polarization interferometry (DPI)

    PubMed Central

    Grøftehauge, Morten K.; Hajizadeh, Nelly R.; Swann, Marcus J.; Pohl, Ehmke

    2015-01-01

    Over the last decades, a wide range of biophysical techniques investigating protein–ligand interactions have become indispensable tools to complement high-resolution crystal structure determinations. Current approaches in solution range from high-throughput-capable methods such as thermal shift assays (TSA) to highly accurate techniques including microscale thermophoresis (MST) and isothermal titration calorimetry (ITC) that can provide a full thermodynamic description of binding events. Surface-based methods such as surface plasmon resonance (SPR) and dual polarization interferometry (DPI) allow real-time measurements and can provide kinetic parameters as well as binding constants. DPI provides additional spatial information about the binding event. Here, an account is presented of new developments and recent applications of TSA and DPI connected to crystallography. PMID:25615858

  10. Enhancement of inflammatory protein expression and nuclear factor Κb (NF-Κb) activity by trichostatin A (TSA) in OP9 preadipocytes.

    PubMed

    Sato, Taiki; Kotake, Daisuke; Hiratsuka, Masahiro; Hirasawa, Noriyasu

    2013-01-01

    The production of inflammatory proteins such as interleukin-6 (IL-6) by preadipocytes and mature adipocytes is closely associated with the impairment of systemic glucose homeostasis. However, precisely how the production is regulated and the roles of histone deacetylases (HDACs) remain largely unknown. The aim of this study was to establish whether HDAC inhibitors affect the expression of inflammatory proteins in pre/mature adipocytes, and, if so, to determine the mechanism involved. Trichostatin A (TSA), an HDAC inhibitor, enhanced lipopolysaccharide (LPS)-induced production of IL-6 in OP9 preadipocytes but not the mature adipocytes. Moreover, TSA also enhanced palmitic acid-induced IL-6 production and the expression of inflammatory genes induced by LPS in preadipocytes. Although TSA did not affect TLR4 mRNA expression or the activation of MAPKs, a reporter gene assay revealed that the LPS-induced increase in nuclear factor κB (NF-κB) activity was enhanced by TSA. Moreover, TSA increased the level of NF-κB p65 acetylation at lysine 310 and duration of its translocation into the nucleus, which leads to enhancement of NF-κB activity and subsequently expression of inflammatory genes. These findings shed new light on the regulatory roles of HDACs in preadipocytes in the production of inflammatory proteins.

  11. BJ-TSA-9, a novel human tumor-specific gene, has potential as a biomarker of lung cancer.

    PubMed

    Li, Yunyan; Dong, Xueyuan; Yin, Yanhui; Su, Yanrong; Xu, Qingwen; Zhang, Yuxia; Pang, Xuewen; Zhang, Yu; Chen, Weifeng

    2005-12-01

    Using bioinformatics, we have identified a novel tumor-specific gene BJ-TSA-9, which has been validated by Northern blot analysis and reverse transcription-polymerase chain reaction (RT-PCR). BJ-TSA-9 mRNA was expressed in 52.5% (21 of 40) of human lung cancer tissues and was especially higher in lung adenocarcinoma (68.8%). To explore the potential application of BJ-TSA-9 for the detection of circulating cancer cells in lung cancer patients, nested RT-PCR was performed. The overall positive detection rate was 34.3% (24 of 70) in peripheral blood mononuclear cells (PBMCs) of patients with various types of lung cancers and was 53.6% (15 of 28) in PBMCs of lung adenocarcinoma patients. In combination with the detection of two known marker genes SCC and LUNX, the detection rate was increased to 81.4%. A follow-up study was performed in 37 patients after surgical removal of tumor mass. Among nine patients with persistent detection of two to three tumor marker transcripts in PBMCs, six patients had recurrence/metastasis. In contrast, 28 patients with transient detection of one tumor marker or without detection of any tumor marker were all in remission. Thus, BJ-TSA-9 may serve as a marker for lung cancer diagnosis and as a marker, in combination with two other tumor markers, for the prediction of the recurrence and prognosis of lung cancer patients.

  12. Effects of Administrator Performance on Student Performance in the Trial State Assessment.

    ERIC Educational Resources Information Center

    McLaughlin, Donald H.

    In 1990 the scope of the National Assessment of Educational Progress (NAEP) was broadened by adding the Trial State Assessment (TSA), in which approximately 2,500 eighth graders in 100 schools in 40 states and U.S. territories participated in the mathematics assessment. A major step was training local test administrators to administer the TSA…

  13. 77 FR 36549 - Nursing Workforce Diversity Invitational Summit-“Nursing in 3D: Workforce Diversity, Health...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-19

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Health Resources and Services Administration Nursing Workforce Diversity Invitational Summit--``Nursing in 3D: Workforce Diversity, Health Disparities, and..., Division of Nursing, will host an invitational summit that focuses on Nursing Workforce Diversity (NWD...

  14. A Novel Compact Wideband TSA Array for Near-Surface Ice Sheet Penetrating Radar Applications

    NASA Astrophysics Data System (ADS)

    Zhang, Feng; Liu, Xiaojun; Fang, Guangyou

    2014-03-01

    A novel compact tapered slot antenna (TSA) array for near-surface ice sheet penetrating radar applications is presented. This TSA array is composed of eight compact antenna elements which are etched on two 480mm × 283mm FR4 substrates. Each antenna element is fed by a wideband coplanar waveguide (CPW) to coupled strip-line (CPS) balun. The two antenna substrates are connected together with a metallic baffle. To obtain wideband properties, another two metallic baffles are used along broadsides of the array. This array is fed by a 1 × 8 wideband power divider. The measured S11 of the array is less than -10dB in the band of 500MHz-2GHz, and the measured gain is more than 6dBi in the whole band which agrees well with the simulated results.

  15. ERNIE performance with TSA portals Initial Results

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Labov, S.

    This project extends the “Enhanced Radiological Nuclear Inspection and Evaluation” (ERNIE) system developed with CBP and DNDO to improve performance of PVT-based Radiation Portal Monitors (RPMs). ERNIE was designed to be used with any RPM system. The first implementation was with the SAIC (Leidos) RPM-8 systems. In this project, we are demonstrating how effective the ERNIE approach can be when applied to the VM250 TSA portals used in NSDD programs. Part of the challenge in adapting ERNIE to handle VM250 portals is the lack of gamma spectral information. We report here on the first results showing how the ERNIE analysismore » can improve analysis of measurements with the VM250 RPMs.« less

  16. [Effect of TSA and VPA treatment on long-tailed macaque (Macaca fascicularis)-pig interspecies somatic cell nuclear transfer].

    PubMed

    Qin, Zu-Xing; Huang, Gao-Bo; Luo, Jun; Ning, Shu-Fang; Lu, Sheng-Sheng; Lu, Ke-Huan

    2012-03-01

    Long-tailed macaque-pig interspecies somatic cell nuclear transfer (iSCNT) is beneficial to yield embryonic stem cells from iSCNT embryos with similar genetic background as human, which can be used as materials for medical and basic research. The primary objective of this study was to investigate the effects of concentrations and treatment duration of two histone deacetylase inhibitors-Trichostatin A (TSA) and Valproic acid (VPA) and two different embryo culture media (PZM-3 and HECM-10) on the in vitro development of iSCNT embryos. The results suggested that when PZM-3 was used as the embryo culture medium, the blastocyst rate of 10 nmol/L TSA treatment for 48 h was significantly higher than the control group (22.78% vs 9.86%, P< 0.05). However, neither in PZM-3 nor in HECM-10, 2-10 mmol/L VPA treatment did not increase the in vitro developmental potential of iSCNT embryos. It was concluded that TSA treatment could enhance the in vitro developmental potential of long-tailed macaque-pig iSCNT embryos.

  17. Modelling the removal of p-TSA (para-toluenesulfonamide) during rapid sand filtration used for drinking water treatment.

    PubMed

    Meffe, Raffaella; Kohfahl, Claus; Holzbecher, Ekkehard; Massmann, Gudrun; Richter, Doreen; Dünnbier, Uwe; Pekdeger, Asaf

    2010-01-01

    A finite element model was set-up to determine degradation rate constants for p-TSA during rapid sand filtration (RSF). Data used for the model originated from a column experiment carried out in the filter hall of a drinking water treatment plant in Berlin (Germany). Aerated abstracted groundwater was passed through a 1.6m long column-shaped experimental sand filter applying infiltration rates from 2 to 6mh(-1). Model results were fitted to measured profiles and breakthrough curves of p-TSA for different infiltration rates using both first-order reaction kinetics and Michaelis-Menten kinetics. Both approaches showed that degradation rates varied both in space and time. Higher degradation rates were observed in the upper part of the column, probably related to higher microbial activity in this zone. Measured and simulated breakthrough curves revealed an adaption phase with lower degradation rates after infiltration rates were changed, followed by an adapted phase with more elevated degradation rates. Irrespective of the mathematical approach and the infiltration rate, degradation rates were very high, probably owing to the fact that filter sands have been in operation for decades, receiving high p-TSA concentrations with the raw water.

  18. TSA suppresses miR-106b-93-25 cluster expression through downregulation of MYC and inhibits proliferation and induces apoptosis in human EMC.

    PubMed

    Zhao, Zhi-Ning; Bai, Jiu-Xu; Zhou, Qiang; Yan, Bo; Qin, Wei-Wei; Jia, Lin-Tao; Meng, Yan-Ling; Jin, Bo-Quan; Yao, Li-Bo; Wang, Tao; Yang, An-Gang

    2012-01-01

    Histone deacetylase (HDAC) inhibitors are emerging as a novel class of anti-tumor agents and have manifested the ability to decrease proliferation and increase apoptosis in different cancer cells. A significant number of genes have been identified as potential effectors responsible for the anti-tumor function of HDAC inhibitor. However, the molecular mechanisms of these HDAC inhibitors in this process remain largely undefined. In the current study, we searched for microRNAs (miRs) that were affected by HDAC inhibitor trichostatin (TSA) and investigated their effects in endometrial cancer (EMC) cells. Our data showed that TSA significantly inhibited the growth of EMC cells and induced their apoptosis. Among the miRNAs that altered in the presence of TSA, the miR-106b-93-25 cluster, together with its host gene MCM7, were obviously down-regulated in EMC cells. p21 and BIM, which were identified as target genes of miR-106b-93-25 cluster, increased in TSA treated tumor cells and were responsible for cell cycle arrest and apoptosis. We further identified MYC as a regulator of miR-106b-93-25 cluster and demonstrated its down-regulation in the presence of TSA resulted in the reduction of miR-106b-93-25 cluster and up-regulation of p21 and BIM. More important, we found miR-106b-93-25 cluster was up-regulated in clinical EMC samples in association with the overexpression of MCM7 and MYC and the down-regulation of p21 and BIM. Thus our studies strongly indicated TSA inhibited EMC cell growth and induced cell apoptosis and cell cycle arrest at least partially through the down-regulation of the miR-106b-93-25 cluster and up-regulation of it's target genes p21 and BIM via MYC.

  19. RUNX3 is involved in caspase-3-dependent apoptosis induced by a combination of 5-aza-CdR and TSA in leukaemia cell lines.

    PubMed

    Zhai, Feng-Xian; Liu, Xiang-Fu; Fan, Rui-Fang; Long, Zi-Jie; Fang, Zhi-Gang; Lu, Ying; Zheng, Yong-Jiang; Lin, Dong-Jun

    2012-03-01

    Epigenetic therapy has had a significant impact on the management of haematologic malignancies. The aim of this study was to assess whether 5-aza-CdR and TSA inhibit the growth of leukaemia cells and induce caspase-3-dependent apoptosis by upregulating RUNX3 expression. K562 and Reh cells were treated with 5-aza-CdR, TSA or both compounds. RT-PCR and Western blot analyses were used to examine the expression of RUNX3 at the mRNA and protein levels, respectively. Immunofluorescence microscopy was used to detect the cellular location of RUNX3. Additionally, after K562 cells were transfected with RUNX3, apoptosis and proliferation were studied using Annexin V staining and MTT assays. The expression of RUNX3 in leukaemia cell lines was markedly less than that in the controls. Demethylating drug 5-aza-CdR could induce RUNX3 expression, but the combination of TSA and 5-aza-CdR had a greater effect than did treatment with a single compound. The combination of 5-aza-CdR and TSA induced the translocation of RUNX3 from the cytoplasm into the nucleus. TSA enhanced apoptosis induced by 5-aza-CdR, and Annexin V and Hoechst 33258 staining showed that the combination induced apoptosis but not necrosis. Furthermore, apoptosis was dependent on the caspase-3 pathway. RUNX3 overexpression in K562 cells led to growth inhibition and apoptosis and potentiated the effects of 5-aza-CdR induction. RUNX3 plays an important role in leukaemia cellular functions, and the induction of RUNX3-mediated effects may contribute to the therapeutic value of combination TSA and 5-aza-CdR treatment.

  20. HDAC inhibitors TSA and sodium butyrate enhanced the human IL-5 expression by altering histone acetylation status at its promoter region.

    PubMed

    Han, Songyan; Lu, Jun; Zhang, Yu; Cheng, Cao; Li, Lin; Han, Liping; Huang, Baiqu

    2007-02-15

    The expression of IL-5 correlated tightly with the maturation and differentiation of eosinophils, and is considered as a cytokine responsible for allergic inflammation. We report here that inhibition of HDAC activity by Trichostatin A (TSA) and sodium butyrate (NaBu), the two specific HDAC inhibitors, resulted in the elevation of both endogenous and exogenous activity of IL-5 promoter. We demonstrated that both the mRNA expression and protein production of IL-5 were stimulated by TSA and NaBu treatments. ChIP assays showed that treatments of TSA and NaBu caused hyperacetylation of histones H3 and H4 on IL-5 promoter in Jurkat cells, which consequently promoted the exogenous luciferase activity driven by this promoter. Moreover, site-directed mutagenesis studies showed that the binding sites for transcription factors NFAT, GATA3 and YY1 on IL-5 promoter were critical for the effects of TSA and NaBu, suggesting that the transcriptional activation of IL-5 gene by these inhibitors was achieved by affecting HDAC function on IL-5 promoter via transcription factors. These data will contribute to elucidating the unique mechanism of IL-5 transcriptional control and to the therapy of allergic disorders related to IL-5.

  1. C-terminal cleavage of DeltaNp63alpha is associated with TSA-induced apoptosis in immortalized corneal epithelial cells.

    PubMed

    Robertson, Danielle M; Ho, Su-Inn; Cavanagh, H Dwight

    2010-08-01

    In the central human corneal epithelium, loss of DeltaNp63 occurs in all surface epithelial cells preparing to undergo desquamation, suggesting a potential role for DeltaNp63 isoforms in mediating surface cell apoptotic shedding. In this study, the authors investigated a role for DeltaNp63 isoforms in caspase-mediated apoptosis in a telomerase-immortalized corneal epithelial cell line. For in vitro studies, hTCEpi cells were cultured in KGM-2 serum-free culture media containing 0.15 mM calcium. To assess dynamic protein interactions among individual DeltaNp63 isoforms, DeltaNp63-EGFP expression plasmids were transiently expressed in hTCEpi cells and evaluated by FRAP. Trichostatin-A (TSA; 3.31 muM) was used to induce cell death as measured by caspase activity. Cleavage and loss of endogenous DeltaNp63alpha, DeltaNp63-EGFP expression plasmids, and p53 were assessed after treatment with TSA and siRNA. Transient expression of DeltaNp63-EGFP alpha and beta isoforms resulted in the formation of a smaller isoform similar in size to DeltaNp63gamma-EGFP. FRAP demonstrated that DeltaNp63alpha-EGFP has greater immobile fraction than beta or gamma. TSA induced caspase-mediated apoptotic pathways; caspase induction was accompanied by a decrease in endogenous DeltaNp63alpha and p53. TSA upregulated DeltaNp63-EGFP plasmid expression; this was accompanied by a selective increase in cleavage of DeltaNp63alpha-EGFP. siRNA knockdown of DeltaNp63alpha correlated with a reduction in p53 independently of TSA. DeltaNp63alpha is the dominant active isoform in corneal epithelial cell nuclei. Loss of DeltaNp63alpha occurs during apoptotic signaling by cleavage at the C terminus. The corresponding loss of p53 suggests that a significant relationship appears to exist between these two regulatory proteins.

  2. Antioxidant proteins TSA and PAG interact synergistically with Presenilin to modulate Notch signaling in Drosophila.

    PubMed

    Wangler, Michael F; Reiter, Lawrence T; Zimm, Georgianna; Trimble-Morgan, Jennifer; Wu, Jane; Bier, Ethan

    2011-07-01

    Alzheimer's disease (AD) pathogenesis is characterized by senile plaques in the brain and evidence of oxidative damage. Oxidative stress may precede plaque formation in AD; however, the link between oxidative damage and plaque formation remains unknown. Presenilins are transmembrane proteins in which mutations lead to accelerated plaque formation and early-onset familial Alzheimer's disease. Presenilins physically interact with two antioxidant enzymes thiol-specific antioxidant (TSA) and proliferation-associated gene (PAG) of the peroxiredoxin family. The functional consequences of these interactions are unclear. In the current study we expressed a presenilin transgene in Drosophila wing and sensory organ precursors of the fly. This caused phenotypes typical of Notch signaling loss-of-function mutations. We found that while expression of TSA or PAG alone produced no phenotype, co-expression of TSA and PAG with presenilin led to an enhanced Notch loss-of-function phenotype. This phenotype was more severe and more penetrant than that caused by the expression of Psn alone. In order to determine whether these phenotypes were indeed affecting Notch signaling, this experiment was performed in a genetic background carrying an activated Notch (Abruptex) allele. The phenotypes were almost completely rescued by this activated Notch allele. These results link peroxiredoxins with the in vivo function of Presenilin, which ultimately connects two key pathogenetic mechanisms in AD, namely, antioxidant activity and plaque formation, and raises the possibility of a role for peroxiredoxin family members in Alzheimer's pathogenesis.

  3. TSA-induced JMJD2B downregulation is associated with cyclin B1-dependent survivin degradation and apoptosis in LNCap cells.

    PubMed

    Zhu, Shan; Li, Yueyang; Zhao, Li; Hou, Pingfu; Shangguan, Chenyan; Yao, Ruosi; Zhang, Weina; Zhang, Yu; Tan, Jiang; Huang, Baiqu; Lu, Jun

    2012-07-01

    Histone deacetylase (HDAC) inhibitors are emerging as a novel class of anti-tumor agents and have manifested the ability to induce apoptosis of cancer cells, and a significant number of genes have been identified as potential effectors responsible for HDAC inhibitor-induced apoptosis. However, the mechanistic actions of these HDAC inhibitors in this process remain largely undefined. We here report that the treatment of LNCap prostate cancer cells with HDAC inhibitor trichostatin A (TSA) resulted in downregulation of the Jumonji domain-containing protein 2B (JMJD2B). We also found that the TSA-mediated decrease in survivin expression in LNCap cells was partly attributable to downregulation of JMJD2B expression. This effect was attributable to the promoted degradation of survivin protein through inhibition of Cyclin B1/Cdc2 complex-mediated survivin Thr34 phosphorylation. Consequently, knockdown of JMJD2B enhanced TSA-induced apoptosis by regulating the Cyclin B1-dependent survivin degradation to potentiate the apoptosis pathways. Copyright © 2012 Wiley Periodicals, Inc.

  4. Protein–ligand interactions investigated by thermal shift assays (TSA) and dual polarization interferometry (DPI)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Grøftehauge, Morten K., E-mail: m.k.groftehauge@durham.ac.uk; Hajizadeh, Nelly R.; Swann, Marcus J.

    2015-01-01

    The biophysical characterization of protein–ligand interactions in solution using techniques such as thermal shift assay, or on surfaces using, for example, dual polarization interferometry, plays an increasingly important role in complementing crystal structure determinations. Over the last decades, a wide range of biophysical techniques investigating protein–ligand interactions have become indispensable tools to complement high-resolution crystal structure determinations. Current approaches in solution range from high-throughput-capable methods such as thermal shift assays (TSA) to highly accurate techniques including microscale thermophoresis (MST) and isothermal titration calorimetry (ITC) that can provide a full thermodynamic description of binding events. Surface-based methods such as surface plasmonmore » resonance (SPR) and dual polarization interferometry (DPI) allow real-time measurements and can provide kinetic parameters as well as binding constants. DPI provides additional spatial information about the binding event. Here, an account is presented of new developments and recent applications of TSA and DPI connected to crystallography.« less

  5. Exploiting multi-function Metal-Organic Framework nanocomposite Ag@Zn-TSA as highly efficient immobilization matrixes for sensitive electrochemical biosensing.

    PubMed

    Dong, Sheying; Zhang, Dandan; Suo, Gaochao; Wei, Wenbo; Huang, Tinglin

    2016-08-31

    A novel multi-function Metal-Organic Framework composite Ag@Zn-TSA (zinc thiosalicylate, Zn(C7H4O2S), Zn-TSA) was synthesized as highly efficient immobilization matrixes of myoglobin (Mb)/glucose oxidase (GOx) for electrochemical biosensing. The electrochemical biosensors based on Ag@Zn-TSA composite and ionic liquid (IL) modified carbon paste electrode (CPE) were fabricated successfully. Furthermore, the properties of the sensors were discussed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and amperometric current-time curve, respectively. The results showed the proposed biosensors had wide linear response to hydrogen peroxide (H2O2) in the range of 0.3-20,000 μM, to nitrite (NO2(-)) for 1.3 μM-1660 μM and 2262 μM-1,33,000 μM, to glucose for 2.0-1022 μM, with a low detection limit of 0.08 μM for H2O2, 0.5 μM for NO2(-), 0.8 μM for glucose. The values of the apparent heterogeneous electron transfer rate constant (ks) for Mb and GOx were estimated as 2.05 s(-1) and 2.45 s(-1), respectively. Thus, Ag@Zn-TSA was a kind of ideal material as highly efficient immobilization matrixes for sensitive electrochemical biosensing. In addition, this work indicated that MOF nanocomposite had a great potential for constructing wide range of sensing interface. Copyright © 2016 Elsevier B.V. All rights reserved.

  6. 77 FR 11145 - Intent to Request Renewal From OMB of One Current Public Collection of Information: Air Cargo...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-24

    ...The Transportation Security Administration (TSA) invites public comment on one currently approved Information Collection Request (ICR), OMB control number 1652-0040, abstracted below that we will submit to the Office of Management and Budget (OMB) for renewal in compliance with the Paperwork Reduction Act. The ICR describes the nature of the information collection and its expected burden. This ICR involves five broad categories of affected populations: airports, passenger aircraft operators, foreign air carriers, indirect air carriers operating under a security program, and all-cargo carriers. The collections of information that make up this ICR are security programs, security threat assessments (STA), known shipper data via the Known Shipper Management System (KSMS), Air Cargo Data Management System (ACDMS), Cargo Reporting Tool for cargo screening reporting, and evidence of compliance recordkeeping. TSA seeks continued OMB approval in order to secure passenger aircraft carrying cargo as authorized in the Aviation and Transportation Security Act.

  7. Separation and Conditioning of Mars Atmospheric Gases via TSA

    NASA Technical Reports Server (NTRS)

    Finn, John E.; Luna, Bernadette (Technical Monitor)

    2000-01-01

    Space and planetary exploration almost always presents interesting and unusual engineering challenges. Separations engineering for chemical processes that are critical to humans working in space is no exception. The challenges are becoming clearer as we make the transition from concepts and planning to hardware development, and as we understand better the constraints and environments in which the processes must perform. The coming decade will see a robotic Mars exploration program that has recovered from recent setbacks and is building a knowledge and technology base for human exploration. One of the missions will carry a small chemical pilot plant for demonstrating the manufacture of rocket propellants and life support consumables from the low-pressure (0.01 atm) Martian atmosphere. By manufacturing and storing the fuel and consumables needed for human-return missions in situ, launch mass and landed mass are reduced by tons and missions become far less expensive. The front-end to the pilot plant is a solid-state atmosphere acquisition and separation unit based on temperature-swing adsorption (TSA). The unit produces purified and pressurized (to 1.0 atm) carbon dioxide to downstream reactors that will make methane and oxygen. The unit also produces a nitrogen-argon mixture as a valuable by-product for life support, inflatable structures, and propellant pressurization. With nighttime temperatures falling to -100 degrees C, power availability restricted to a few watts, and flawless operation critical to success, the dusty Martian surface is a difficult place to operate a remote plant. This talk will focus on how this TSA separation process is designed and implemented for this application, and how it might be used in the more distant future for human exploration.

  8. Functional link between DNA damage responses and transcriptional regulation by ATM in response to a histone deacetylase inhibitor TSA.

    PubMed

    Lee, Jong-Soo

    2007-09-01

    Mutations in the ATM (ataxia-telangiectasia mutated) gene, which encodes a 370 kd protein with a kinase catalytic domain, predisposes people to cancers, and these mutations are also linked to ataxia-telangiectasia (A-T). The histone acetylaion/deacetylation- dependent chromatin remodeling can activate the ATM kinase-mediated DNA damage signal pathway (in an accompanying work, Lee, 2007). This has led us to study whether this modification can impinge on the ATM-mediated DNA damage response via transcriptional modulation in order to understand the function of ATM in the regulation of gene transcription. To identify the genes whose expression is regulated by ATM in response to histone deaceylase (HDAC) inhibition, we performed an analysis of oligonucleotide microarrays with using the appropriate cell lines, isogenic A-T (ATM(-)) and control (ATM(+)) cells, following treatment with a HDAC inhibitor TSA. Treatment with TSA reprograms the differential gene expression profile in response to HDAC inhibition in ATM(-) cells and ATM(+) cells. We analyzed the genes that are regulated by TSA in the ATM-dependent manner, and we classified these genes into different functional categories, including those involved in cell cycle/DNA replication, DNA repair, apoptosis, growth/differentiation, cell- cell adhesion, signal transduction, metabolism and transcription. We found that while some genes are regulated by TSA without regard to ATM, the patterns of gene regulation are differentially regulated in an ATM-dependent manner. Taken together, these finding indicate that ATM can regulate the transcription of genes that play critical roles in the molecular response to DNA damage, and this response is modulated through an altered HDAC inhibition-mediated gene expression.

  9. Can Thermally Sprayed Aluminum (TSA) Mitigate Corrosion of Carbon Steel in Carbon Capture and Storage (CCS) Environments?

    NASA Astrophysics Data System (ADS)

    Paul, S.; Syrek-Gerstenkorn, B.

    2017-01-01

    Transport of CO2 for carbon capture and storage (CCS) uses low-cost carbon steel pipelines owing to their negligible corrosion rates in dry CO2. However, in the presence of liquid water, CO2 forms corrosive carbonic acid. In order to mitigate wet CO2 corrosion, use of expensive corrosion-resistant alloys is recommended; however, the increased cost makes such selection economically unfeasible; hence, new corrosion mitigation methods are sought. One such method is the use of thermally sprayed aluminum (TSA), which has been used to mitigate corrosion of carbon steel in seawater, but there are concerns regarding its suitability in CO2-containing solutions. A 30-day test was carried out during which carbon steel specimens arc-sprayed with aluminum were immersed in deionized water at ambient temperature bubbled with 0.1 MPa CO2. The acidity (pH) and potential were continuously monitored, and the amount of dissolved Al3+ ions was measured after completion of the test. Some dissolution of TSA occurred in the test solution leading to nominal loss in coating thickness. Potential measurements revealed that polarity reversal occurs during the initial stages of exposure which could lead to preferential dissolution of carbon steel in the case of coating damage. Thus, one needs to be careful while using TSA in CCS environments.

  10. Kinetic and Thermodynamic Rationale for SAHA Being a Preferential Human HDAC8 Inhibitor as Compared to the Structurally Similar Ligand, TSA

    PubMed Central

    Singh, Raushan K.; Lall, Naveena; Leedahl, Travis S.; McGillivray, Abigail; Mandal, Tanmay; Haldar, Manas; Mallik, Sanku; Cook, Gregory; Srivastava, D.K.

    2013-01-01

    Of the different hydroxamate-based histone deacetylase (HDAC) inhibitors, Suberoylanilide hydroxamic acid (SAHA) has been approved by the FDA for treatment of T-cell lymphoma. Interestingly, a structurally similar inhibitor, Trichostatin A (TSA), which has a higher in vitro inhibitory-potency against HDAC8, reportedly shows a poor efficacy in clinical settings. In order to gain the molecular insight into the above discriminatory feature, we performed transient kinetic and isothermal titration calorimetric studies for the interaction of SAHA and TSA to the recombinant form of human HDAC8. The transient kinetic data revealed that the binding of both the inhibitors to the enzyme showed the biphasic profiles, which represented an initial encounter of enzyme with the inhibitor followed by the isomerization of the transient enzyme-inhibitor complexes. The temperature-dependent transient kinetic studies with the above inhibitors revealed that the bimolecular process is primarily dominated by favorable enthalpic changes, as opposed to the isomerization step; which is solely contributed by entropic changes. The standard binding-enthalpy (ΔH0) of SAHA, deduced from the transient kinetic as well as the isothermal titration calorimetric experiments, was 2–3 kcal/mol higher as compared to TSA. The experimental data presented herein suggests that SAHA serves as a preferential (target-specific/selective) HDAC8 inhibitor as compared to TSA. Arguments are presented that the detailed kinetic and thermodynamic studies may guide in the rational design of HDAC inhibitors as therapeutic agents. PMID:24079912

  11. Loss of vacuolar H+-ATPase (V-ATPase) activity in yeast generates an iron deprivation signal that is moderated by induction of the peroxiredoxin TSA2.

    PubMed

    Diab, Heba I; Kane, Patricia M

    2013-04-19

    Vacuolar H(+)-ATPases (V-ATPases) acidify intracellular organelles and help to regulate overall cellular pH. Yeast vma mutants lack V-ATPase activity and allow exploration of connections between cellular pH, iron, and redox homeostasis common to all eukaryotes. A previous microarray study in a vma mutant demonstrated up-regulation of multiple iron uptake genes under control of Aft1p (the iron regulon) and only one antioxidant gene, the peroxiredoxin TSA2 (Milgrom, E., Diab, H., Middleton, F., and Kane, P. M. (2007) Loss of vacuolar proton-translocating ATPase activity in yeast results in chronic oxidative stress. J. Biol. Chem. 282, 7125-7136). Fluorescent biosensors placing GFP under transcriptional control of either an Aft1-dependent promoter (P(FIT2)-GFP) or the TSA2 promoter (P(TSA2)-GFP) were constructed to monitor transcriptional signaling. Both biosensors were up-regulated in the vma2Δ mutant, and acute V-ATPase inhibition with concanamycin A induced coordinate up-regulation from both promoters. PTSA2-GFP induction was Yap1p-dependent, indicating an oxidative stress signal. Total cell iron measurements indicate that the vma2Δ mutant is iron-replete, despite up-regulation of the iron regulon. Acetic acid up-regulated P(FIT2)-GFP expression in wild-type cells, suggesting that loss of pH control contributes to an iron deficiency signal in the mutant. Iron supplementation significantly decreased P(FIT2)-GFP expression and, surprisingly, restored P(TSA2)-GFP to wild-type levels. A tsa2Δ mutation induced both nuclear localization of Aft1p and P(FIT2)-GFP expression. The data suggest a novel function for Tsa2p as a negative regulator of Aft1p-driven transcription, which is induced in V-ATPase mutants to limit transcription of the iron regulon. This represents a new mechanism bridging the antioxidant and iron-regulatory pathways that is intimately linked to pH homeostasis.

  12. Sp1 and Sp3 Are the Transcription Activators of Human ek1 Promoter in TSA-Treated Human Colon Carcinoma Cells.

    PubMed

    Kuan, Chee Sian; See Too, Wei Cun; Few, Ling Ling

    2016-01-01

    Ethanolamine kinase (EK) catalyzes the phosphorylation of ethanolamine, the first step in the CDP-ethanolamine pathway for the biosynthesis of phosphatidylethanolamine (PE). Human EK exists as EK1, EK2α and EK2β isoforms, encoded by two separate genes, named ek1 and ek2. EK activity is stimulated by carcinogens and oncogenes, suggesting the involvement of EK in carcinogenesis. Currently, little is known about EK transcriptional regulation by endogenous or exogenous signals, and the ek gene promoter has never been studied. In this report, we mapped the important regulatory regions in the human ek1 promoter. 5' deletion analysis and site-directed mutagenesis identified a Sp site at position (-40/-31) that was essential for the basal transcription of this gene. Treatment of HCT116 cells with trichostatin A (TSA), a histone deacetylase inhibitor, significantly upregulated the ek1 promoter activity through the Sp(-40/-31) site and increased the endogenous expression of ek1. Chromatin immunoprecipitation assay revealed that TSA increased the binding of Sp1, Sp3 and RNA polymerase II to the ek1 promoter in HCT116 cells. The effect of TSA on ek1 promoter activity was cell-line specific as TSA treatment did not affect ek1 promoter activity in HepG2 cells. In conclusion, we showed that Sp1 and Sp3 are not only essential for the basal transcription of the ek1 gene, their accessibility to the target site on the ek1 promoter is regulated by histone protein modification in a cell line dependent manner.

  13. Detection of single-copy functional genes in prokaryotic cells by two-pass TSA-FISH with polynucleotide probes.

    PubMed

    Kawakami, Shuji; Hasegawa, Takuya; Imachi, Hiroyuki; Yamaguchi, Takashi; Harada, Hideki; Ohashi, Akiyoshi; Kubota, Kengo

    2012-02-01

    In situ detection of functional genes with single-cell resolution is currently of interest to microbiologists. Here, we developed a two-pass tyramide signal amplification (TSA)-fluorescence in situ hybridization (FISH) protocol with PCR-derived polynucleotide probes for the detection of single-copy genes in prokaryotic cells. The mcrA gene and the apsA gene in methanogens and sulfate-reducing bacteria, respectively, were targeted. The protocol showed bright fluorescence with a good signal-to-noise ratio and achieved a high efficiency of detection (>98%). The discrimination threshold was approximately 82-89% sequence identity. Microorganisms possessing the mcrA or apsA gene in anaerobic sludge samples were successfully detected by two-pass TSA-FISH with polynucleotide probes. The developed protocol is useful for identifying single microbial cells based on functional gene sequences. Copyright © 2011 Elsevier B.V. All rights reserved.

  14. 76 FR 30236 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-24

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  1. 76 FR 55734 - Requested Administrative Waiver of the Coastwise Trade Laws

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    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-26

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2011 0068] Requested Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws...

  16. 76 FR 18299 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-01

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket Number MARAD 2011 0034] Requested Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws...

  17. Localization of human coagulation factor VIII (hFVIII) in transgenic rabbit by FISH-TSA: identification of transgene copy number and transmission to the next generation.

    PubMed

    Krylov, V; Tlapáková, T; Mácha, J; Curlej, J; Ryban, L; Chrenek, P

    2008-01-01

    For chromosomal localization of the hFVIII human transgene in F2 and F3 generation of transgenic rabbits, FISH-TSA was applied. A short cDNA probe (1250 bp) targeted chromosomes 3, 7, 8, 9 and 18 of an F2 male (animal 1-3-8). Two transgenic offspring (F3) revealed signal positions in chromosome 3 and chromosomes 3 and 7, respectively. Sequencing and structure analysis of the rabbit orthologous gene revealed high similarity to its human counterpart. Part of the sequenced cDNA (1310 bp) served as a probe for FISH-TSA analysis. The rabbit gene was localized in the q arm terminus of the X chromosome. This result is in agreement with reciprocal chromosome painting between the rabbit and the human. The presented FISH-TSA method provides strong signals without any interspecies reactivity.

  18. 78 FR 45256 - Intent To Request Approval From OMB of One New Public Collection of Information: TSA Pre✓TM

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-26

    ...-begins . TSA seeks to establish enrollment sites and implement a mobile enrollment capability. Those... by submitting biographic information and paying the fee using a secure web portal (or by money order...

  19. Oh to Be Rid of Administrative Wimps!

    ERIC Educational Resources Information Center

    Weissberg, Robert

    2007-01-01

    Contemporary university administrators are usually wimps, and timidity in facing easily enraged campus radicals only invites outrageous demands which, in turn, subvert intellectual life. This must be changed. For much of human history leaders had to display physical valor and this trait should be restored to the college administrator job…

  20. A Model for Administrative Evaluation by Subordinates.

    ERIC Educational Resources Information Center

    Budig, Jeanne E.

    Under the administrator evaluation program adopted at Vincennes University, all faculty and professional staff are invited to evaluate each administrator above them in the chain of command. Originally based on the Purdue University "cafeteria" system, this evaluation model has been used biannually for 10 years. In an effort to simplify the system,…

  1. Synergistic anticancer effects of cisplatin and histone deacetylase inhibitors (SAHA and TSA) on cholangiocarcinoma cell lines.

    PubMed

    Asgar, Md Ali; Senawong, Gulsiri; Sripa, Banchob; Senawong, Thanaset

    2016-01-01

    Clinical application of cisplatin against cholangiocarcinoma is often associated with resistance and toxicity posing urgent demand for combination therapy. In this study, we evaluated the combined anticancer effect of cisplatin and histone deacetylase inhibitors (HDACIs), suberoylanilide hydroxamic acid (SAHA) and trichostatin A (TSA), on the cholangiocarcinoma KKU-100 and KKU-M214 cell lines. Antiproliferative activity was evaluated using MTT assay. Apoptosis induction and cell cycle arrest were analyzed by flow cytometry. Cell cycle and apoptosis regulating proteins were evaluated by western blot analysis. MTT assay showed that cisplatin, SAHA and TSA dose-dependently reduced the viability of KKU-100 and KKU-M214 cells. The combination of cisplatin and HDACIs exerted significantly more cytotoxicity than the single drugs. Combination indices below 1.0 reflect synergism between cisplatin and HDACIs, leading to positive dose reductions of cisplatin and HDACIs. Cisplatin and HDACIs alone induced G0/G1 phase arrest in KKU-100 cells, but the drug combinations increased sub-G1 percent more than either drug. However, cisplatin and HDACIs alone or in combination increased only the sub-G1 percent in KKU-M214 cells. Annexin V-FITC staining revealed that cisplatin and HDACIs combinations induced more apoptotic cell death of both KKU-100 and KKU-M214 cells than the single drug. In KKU-100 cells, growth inhibition was accompanied by upregulation of p53 and p21 and downregulation of CDK4 and Bcl-2 due to exposure to cisplatin, SAHA and TSA alone or in combination. Moreover, combination of agents exerted higher impacts on protein expression. Single agents or combination did not affect p53 expression, however, combination of cisplatin and HDACIs increased the expression of p21 in KKU-M214 cells. Taken together, cisplatin and HDACIs combination may improve the therapeutic outcome in cholangiocarcinoma patients.

  2. 75 FR 42481 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-21

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, DOT. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws for the vessel SEAFLYER... Administration (MARAD), is authorized to grant waivers of the U.S.-build requirement of the coastwise laws under...

  3. 75 FR 44757 - Notice of Funding Availability (NOFA) Inviting Applications for the Rural Microentrepreneur...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-29

    ... DEPARTMENT OF AGRICULTURE Rural Business-Cooperative Service Notice of Funding Availability (NOFA) Inviting Applications for the Rural Microentrepreneur Assistance Program AGENCY: Rural Business-Cooperative.... Canales, Administrator, Rural Business-Cooperative Service. [FR Doc. 2010-18639 Filed 7-28-10; 8:45 am...

  4. 76 FR 30237 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-24

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  5. 75 FR 54960 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-09

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  6. 76 FR 35944 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  7. 75 FR 28101 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-19

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  8. 76 FR 51464 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-18

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  9. 76 FR 29332 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-20

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  10. 76 FR 6184 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-03

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  11. 76 FR 51467 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-18

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  12. 75 FR 9021 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-26

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  13. 76 FR 13701 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-14

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  14. 75 FR 11991 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-12

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  15. 75 FR 38865 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-06

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  16. 76 FR 11559 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-02

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  17. 76 FR 30236 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-24

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  18. 75 FR 70069 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-16

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  19. 76 FR 820 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-06

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  20. 76 FR 51465 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-18

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  1. 76 FR 30755 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-26

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  2. 75 FR 14487 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-25

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  3. 76 FR 38269 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-29

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Department of Transportation, Maritime Administration. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  4. 75 FR 5171 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  5. 75 FR 14486 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-25

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  6. 75 FR 5172 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  7. 76 FR 11561 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-02

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  8. 76 FR 13702 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-14

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  9. 76 FR 51466 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-18

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  10. 76 FR 6183 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-03

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  11. 75 FR 6433 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-09

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  12. 76 FR 30235 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-24

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  13. 76 FR 11560 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-02

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  14. 76 FR 4419 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-25

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  15. 76 FR 29331 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-20

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  16. 76 FR 6182 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-03

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  17. 75 FR 25924 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-10

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  18. 75 FR 9021 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-26

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  19. 76 FR 13700 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-14

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  20. 76 FR 46893 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  1. 76 FR 51465 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-18

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  2. 76 FR 35943 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  3. 76 FR 11558 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-02

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... the U.S.-build requirement of the coastwise laws under certain circumstances. A request for such a...

  4. 75 FR 38864 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-06

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  5. 76 FR 11558 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-02

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  6. 75 FR 70068 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-16

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  7. 75 FR 35517 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-22

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  8. 75 FR 25924 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-10

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  9. 75 FR 14485 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-25

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  10. 75 FR 9020 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-26

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  11. 75 FR 49553 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-13

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  12. 75 FR 47679 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-06

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  13. 75 FR 9020 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-26

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  14. 76 FR 51466 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-18

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  15. 76 FR 819 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-06

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  16. 76 FR 35944 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  17. 76 FR 46894 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  18. 75 FR 60862 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-01

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  19. 76 FR 39152 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-05

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  20. 76 FR 35945 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  1. 76 FR 35946 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  2. 76 FR 46894 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  3. 75 FR 2592 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-15

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  4. 75 FR 75545 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-03

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws.... build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  5. 76 FR 40984 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-12

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  6. 76 FR 40983 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-12

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  7. 75 FR 75543 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-03

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws.... build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  8. 75 FR 56655 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-16

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  9. 75 FR 56656 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-16

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  10. 76 FR 9402 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-17

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  11. 76 FR 40982 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-12

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received...

  12. 76 FR 40981 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-12

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....- build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  13. 76 FR 40983 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-12

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  14. 75 FR 52064 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-24

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  15. 75 FR 75544 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-03

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  16. 75 FR 2591 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-15

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, Department of Transportation. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws....-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has...

  17. 75 FR 42481 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-21

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, DOT. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws for the vessel... of the coastwise laws under certain circumstances. A request for such a waiver has been received by...

  18. 75 FR 42480 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-21

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, DOT. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws for the vessel THIRD... coastwise laws under certain circumstances. A request for such a waiver has been received by MARAD. The...

  19. 75 FR 42480 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-21

    ... Administrative Waiver of the Coastwise Trade Laws AGENCY: Maritime Administration, DOT. ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws for the vessel DIAMOND... coastwise laws under certain circumstances. A request for such a waiver has been received by MARAD. The...

  20. GPS Block 2R Time Standard Assembly (TSA) architecture

    NASA Technical Reports Server (NTRS)

    Baker, Anthony P.

    1990-01-01

    The underlying philosophy of the Global Positioning System (GPS) 2R Time Standard Assembly (TSA) architecture is to utilize two frequency sources, one fixed frequency reference source and one system frequency source, and to couple the system frequency source to the reference frequency source via a sample data loop. The system source is used to provide the basic clock frequency and timing for the space vehicle (SV) and it uses a voltage controlled crystal oscillator (VCXO) with high short term stability. The reference source is an atomic frequency standard (AFS) with high long term stability. The architecture can support any type of frequency standard. In the system design rubidium, cesium, and H2 masers outputting a canonical frequency were accommodated. The architecture is software intensive. All VCXO adjustments are digital and are calculated by a processor. They are applied to the VCXO via a digital to analog converter.

  1. Invitational Education: Theory, Research and Practice

    ERIC Educational Resources Information Center

    Haigh, Martin

    2011-01-01

    Invitational Theory argues that learning is enhanced when learners are positively encouraged or "invited" into the educational experience. Arising from perceptual and self-concept theory, Invitational Pedagogy is constructed on four principles: respect for people, trust, optimism and intentionality, and upon five pillars: people, places, policies,…

  2. Vaccination with plasmid DNA encoding TSA/LmSTI1 leishmanial fusion proteins confers protection against Leishmania major infection in susceptible BALB/c mice.

    PubMed

    Campos-Neto, A; Webb, J R; Greeson, K; Coler, R N; Skeiky, Y A W; Reed, S G

    2002-06-01

    We have recently shown that a cocktail containing two leishmanial recombinant antigens (LmSTI1 and TSA) and interleukin-12 (IL-12) as an adjuvant induces solid protection in both a murine and a nonhuman primate model of cutaneous leishmaniasis. However, because IL-12 is difficult to prepare, is expensive, and does not have the stability required for a vaccine product, we have investigated the possibility of using DNA as an alternative means of inducing protective immunity. Here, we present evidence that the antigens TSA and LmSTI1 delivered in a plasmid DNA format either as single genes or in a tandem digene construct induce equally solid protection against Leishmania major infection in susceptible BALB/c mice. Immunization of mice with either TSA DNA or LmSTI1 DNA induced specific CD4(+)-T-cell responses of the Th1 phenotype without a requirement for specific adjuvant. CD8 responses, as measured by cytotoxic-T-lymphocyte activity, were generated after immunization with TSA DNA but not LmSTI1 DNA. Interestingly, vaccination of mice with TSA DNA consistently induced protection to a much greater extent than LmSTI1 DNA, thus supporting the notion that CD8 responses might be an important accessory arm of the immune response for acquired resistance against leishmaniasis. Moreover, the protection induced by DNA immunization was specific for infection with Leishmania, i.e., the immunization had no effect on the course of infection of the mice challenged with an unrelated intracellular pathogen such as Mycobacterium tuberculosis. Conversely, immunization of BALB/c mice with a plasmid DNA that is protective against challenge with M. tuberculosis had no effect on the course of infection of these mice with L. major. Together, these results indicate that the protection observed with the leishmanial DNA is mediated by acquired specific immune response rather than by the activation of nonspecific innate immune mechanisms. In addition, a plasmid DNA containing a fusion construct

  3. 75 FR 19610 - Solid Urea From the Russian Federation: Preliminary Results of Antidumping Duty Administrative...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-15

    ... Federation: Preliminary Results of Antidumping Duty Administrative Review AGENCY: Import Administration.... We invite interested parties to comment on these preliminary results. Parties who submit argument in...

  4. At the Tipping Point: Navigating the Course for the Preparation of Educational Administrators. The 2007 Yearbook of the National Council of Professors of Educational Administration

    ERIC Educational Resources Information Center

    Lemasters, Linda K., Ed.; Papa, Rosemary, Ed.

    2007-01-01

    This volume presents the 2007 Yearbook of the National Council of Professors of Educational Administration (NCPEA). This year's theme is "At the Tipping Point: Navigating the Course for the Preparation of Educational Administrators." This yearbook contains six parts. Part 1, Invited Chapters, includes the following: (1) President's…

  5. Trypanosoma cruzi vaccine candidate antigens Tc24 and TSA-1 recall memory immune response associated with HLA-A and -B supertypes in Chagasic chronic patients from Mexico.

    PubMed

    Villanueva-Lizama, Liliana E; Cruz-Chan, Julio V; Aguilar-Cetina, Amarú Del C; Herrera-Sanchez, Luis F; Rodriguez-Perez, Jose M; Rosado-Vallado, Miguel E; Ramirez-Sierra, Maria J; Ortega-Lopez, Jaime; Jones, Kathryn; Hotez, Peter; Bottazzi, Maria Elena; Dumonteil, Eric

    2018-01-01

    Trypanosoma cruzi antigens TSA-1 and Tc24 have shown promise as vaccine candidates in animal studies. We evaluated here the recall immune response these antigens induce in Chagasic patients, as a first step to test their immunogenicity in humans. We evaluated the in vitro cellular immune response after stimulation with recombinant TSA-1 (rTSA-1) or recombinant Tc24 (rTc24) in mononuclear cells of asymptomatic Chagasic chronic patients (n = 20) compared to healthy volunteers (n = 19) from Yucatan, Mexico. Proliferation assays, intracellular cytokine staining, cytometric bead arrays, and memory T cell immunophenotyping were performed by flow cytometry. Peripheral blood mononuclear cells (PBMC) from Chagasic patients showed significant proliferation after stimulation with rTc24 and presented a phenotype of T effector memory cells (CD45RA-CCR7-). These cells also produced IFN-γ and, to a lesser extent IL10, after stimulation with rTSA-1 and rTc24 proteins. Overall, both antigens recalled a broad immune response in some Chagasic patients, confirming that their immune system had been primed against these antigens during natural infection. Analysis of HLA-A and HLA-B allele diversity by PCR-sequencing indicated that HLA-A03 and HLA-B07 were the most frequent supertypes in this Mexican population. Also, there was a significant difference in the frequency of HLA-A01 and HLA-A02 supertypes between Chagasic patients and controls, while the other alleles were evenly distributed. Some aspects of the immune response, such as antigen-induced IFN-γ production by CD4+ and CD8+ T cells and CD8+ proliferation, showed significant association with specific HLA-A supertypes, depending on the antigen considered. In conclusion, our results confirm the ability of both TSA-1 and Tc24 recombinant proteins to recall an immune response induced by the native antigens during natural infection in at least some patients. Our data support the further development of these antigens as therapeutic

  6. Role of glycogen synthase kinase 3 beta (GSK3beta) in mediating the cytotoxic effects of the histone deacetylase inhibitor trichostatin A (TSA) in MCF-7 breast cancer cells.

    PubMed

    Alao, John P; Stavropoulou, Alexandra V; Lam, Eric W-F; Coombes, R Charles

    2006-10-03

    Histone deacetylase inhibitors (HDACIs) have been shown to induce apoptotic and autophagic cell death in vitro and in vivo. The molecular mechanisms that underlie these cytotoxic effects are not yet clearly understood. Recently, HDACIs were shown to induce Akt dephosphorylation by disrupting HDAC-protein phosphatase 1 (PP1) complexes. This disruption results in the increased association of PP1 with Akt, resulting in the dephosphorylation and consequent inactivation of the kinase. Akt enhances cellular survival through the phosphorylation-dependent inhibition of several pro-apoptotic proteins. Akt is an important negative regulator of GSK3beta, a kinase that has been shown to regulate apoptosis in response to various stimuli. In the present study, we investigated the role of GSK3beta in mediating the cytotoxic effects in MCF-7 breast cancer cells treated with trichostatin A (TSA), a prototype HDACI. We show that TSA induces Akt dephosphorylation in a PP1-dependent manner, resulting in activation of GSK3beta in MCF-7 cells. Similarly, knockdown of HDAC1 and-2 by small interfering RNA (siRNA) resulted in the dephosphorylation of Akt and GSK3beta. Selective inhibition of GSK3beta attenuated TSA induced cytotoxicity and resulted in enhanced proliferation following drug removal. Our findings identify GSK3beta as an important mediator of TSA-induced cytotoxicity in MCF-7 breast cancer cells.

  7. 77 FR 513 - Intent To Request Renewal From OMB of One Current Public Collection of Information; Maryland...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-05

    ...The Transportation Security Administration (TSA) invites public comment on one currently approved Information Collection Request (ICR), Office of Management and Budget (OMB) control number 1652-0029, abstracted below that we will submit to OMB for renewal in compliance with the Paperwork Reduction Act (PRA). The ICR describes the nature of the information collection and its expected burden. This collection requires individuals to successfully complete a security threat assessment in order to operate an aircraft to or from one of the three Maryland airports that are located within the Washington, DC, Metropolitan Area Flight Restricted Zone (Maryland Three Airports), or to serve as an airport security coordinator at one of these three airports.

  8. Pitfalls using tyramide signal amplification (TSA) in the mouse gastrointestinal tract: endogenous streptavidin-binding sites lead to false positive staining.

    PubMed

    Horling, L; Neuhuber, W L; Raab, M

    2012-02-15

    Highly sensitive immunohistochemical detection systems such as tyramide signal amplification (TSA) are widely used, since they allow using two primary antibodies raised in the same species. Most of them are based on the streptavidin-biotin-peroxidase system and include streptavidin-coupled secondary antibodies. Using TSA in cryostat-sectioned tissues of mouse esophagus, we were puzzled by negative controls with unexpected staining mostly in the ganglionic areas. This prompted us to search for the causing agent and to include also other parts of the mouse gastrointestinal tract for comparison. Streptavidin-coupled antibodies bound to endogenous binding sites yet to be characterized, which are present throughout the mouse intestines. Staining was mainly localized around neuronal cell bodies of enteric ganglia. Thus, caution is warranted when applying streptavidin-coupled antibodies in the mouse gastrointestinal tract. The use of endogenous biotin-blocking kits combined with a prolonged post-fixation time could significantly reduce unintentional staining. Copyright © 2011 Elsevier B.V. All rights reserved.

  9. 49 CFR 1546.207 - Screening of individuals and property.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY FOREIGN AIR CARRIER.... As required in its security program, each foreign air carrier must ensure that all individuals or... TSA is conducting screening using TSA employees or when using companies under contract with TSA. (c...

  10. 49 CFR 1546.207 - Screening of individuals and property.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY FOREIGN AIR CARRIER.... As required in its security program, each foreign air carrier must ensure that all individuals or... TSA is conducting screening using TSA employees or when using companies under contract with TSA. (c...

  11. 49 CFR 1546.207 - Screening of individuals and property.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY FOREIGN AIR CARRIER.... As required in its security program, each foreign air carrier must ensure that all individuals or... TSA is conducting screening using TSA employees or when using companies under contract with TSA. (c...

  12. 49 CFR 1546.207 - Screening of individuals and property.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY FOREIGN AIR CARRIER.... As required in its security program, each foreign air carrier must ensure that all individuals or... TSA is conducting screening using TSA employees or when using companies under contract with TSA. (c...

  13. p-TSA/Base-Promoted Propargylation/Cyclization of β-Ketothioamides for the Regioselective Synthesis of Highly Substituted (Hydro)thiophenes.

    PubMed

    Nandi, Ganesh Chandra; Singh, Maya Shankar

    2016-07-15

    Metal-free, p-toluenesulfonic acid (p-TSA)-mediated, straightforward propargylation of β-ketothioamides with aryl propargyl alcohol has been achieved at room temperature. In addition, the reaction also provided thiazole rings as byproducts. Furthermore, the propargylated thioamides undergo intramolecular 1,5-cyclization to afford fully substituted (hydro)thiophenes in the presence of base. Notably, the approach is pot, atom, and step economical (PASE).

  14. A combination of exosomes carrying TSA derived from HLA-A2-positive human white buffy coat and polyI:C for use as a subcellular antitumor vaccination.

    PubMed

    Ren, Wei-na; Chang, Chun-kang; Fan, Hua-hua; Guo, Fang; Ren, Ya-na; Yang, Jie; Guo, Juan; Li, Xiao

    2011-01-01

    To improve its antitumor effect, we used human leukocyte antigen -A2 (HLA-A2)-positive human dendritic cell (DC)-derived DEXs (DC-derived exosomes) to support NY-ESO-1 antigen and polyI:C, with the aim of increasing the proliferation of specific cytotoxic T lymphocytes (CTL) in transgenic mice. Mature dendritic cells derived from peripheral blood mononuclear cells (PBMC) were isolated from the blood of healthy adults with positive HLA-2A. Using centrifuge and membrane ultrafiltration, EXO (exosomes) were extracted from the supernatant of DCs secretions. Transgenic C57 mice were immunized with human-derived tumor testis antigen NY-ESO-1/EXO, with or without polyI:C. Mice were sacrificed four weeks after immunization, and spleen cells were isolated and tested for function. The experiments included antigen-specific CTL proliferation, as tested by dimerization and antitumor effects for K562 cells as well as melanoma, tested at different ratios of effected cells:target cells (0:1, 10:1, 50:1, and 100:1). Dimerization experiments indicated that the effect of DEX/TSA (tumor specific antigens) + PolyI:C was 2.36 ± 1.10% and the control was 0.38 ± 0.31%, while the effect of DEX/TSA was 1.97 ± 0.63% and the control was 0.36 ± 0.07%. Antitumor effects by DEX/TSA: PolyI:C for the cell ratios of 0:1, 10:1, 50:1, and 100:1 were 11.14 ± 1.36%, 14.17 ± 0.62%, 15.71 ± 2.48%, and 24.31 ± 2.91%, respectively, for K562 cells. The antitumor effects for DEX/TSA for the cell ratios of 0:1, 10:1, 50:1, and 100:1 were 12.23 ± 2.25%, 13.10 ± 1.57%, 15.27 ± 2.93%, and 19.87 ± 2.72%, respectively, for K562 cells. With ratios of 10:1 and 100:1, the antitumor effects of DEX/TSA + PolyI:C were better than for the DEX/TSA group (P < 0.05). However, higher ratios of effecter cells to target cells increased, and there were no significant improvements in antitumor effect for control cells. Combining PolyI:C with DEX/TSA derived from healthy human blood

  15. 20 CFR 633.202 - Announcement of State planning estimates and invitation to submit a grant application.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Announcement of State planning estimates and invitation to submit a grant application. 633.202 Section 633.202 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR MIGRANT AND SEASONAL FARMWORKER PROGRAMS Grant Planning and Application...

  16. Eastern Sources of Invitational Education.

    ERIC Educational Resources Information Center

    Ryback, David

    1993-01-01

    Presents historical perspective suggesting that invitational theory shares many beliefs with ancient Eastern philosophies. Submits that teachers and other educators who embrace the invitational perspective may benefit from an understanding of Eastern principles. Briefly describes Buddhism, Confucianism, Taoism, Zen Buddhism, and their relevance to…

  17. 11 CFR 100.137 - Invitations, food, and beverages.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 11 Federal Elections 1 2010-01-01 2010-01-01 false Invitations, food, and beverages. 100.137...) Exceptions to Expenditures § 100.137 Invitations, food, and beverages. The cost of invitations, food, and... invitations, food and beverages provided by the individual on behalf of the candidate does not exceed $1,000...

  18. 11 CFR 100.137 - Invitations, food, and beverages.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 11 Federal Elections 1 2014-01-01 2014-01-01 false Invitations, food, and beverages. 100.137...) Exceptions to Expenditures § 100.137 Invitations, food, and beverages. The cost of invitations, food, and... invitations, food and beverages provided by the individual on behalf of the candidate does not exceed $1,000...

  19. 11 CFR 100.137 - Invitations, food, and beverages.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 11 Federal Elections 1 2012-01-01 2012-01-01 false Invitations, food, and beverages. 100.137...) Exceptions to Expenditures § 100.137 Invitations, food, and beverages. The cost of invitations, food, and... invitations, food and beverages provided by the individual on behalf of the candidate does not exceed $1,000...

  20. 11 CFR 100.77 - Invitations, food, and beverages.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 11 Federal Elections 1 2014-01-01 2014-01-01 false Invitations, food, and beverages. 100.77...) Exceptions to Contributions § 100.77 Invitations, food, and beverages. The cost of invitations, food and... invitations, food and beverages provided by the individual on behalf of the candidate does not exceed $1,000...

  1. 11 CFR 100.77 - Invitations, food, and beverages.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 11 Federal Elections 1 2012-01-01 2012-01-01 false Invitations, food, and beverages. 100.77...) Exceptions to Contributions § 100.77 Invitations, food, and beverages. The cost of invitations, food and... invitations, food and beverages provided by the individual on behalf of the candidate does not exceed $1,000...

  2. 11 CFR 100.77 - Invitations, food, and beverages.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 11 Federal Elections 1 2010-01-01 2010-01-01 false Invitations, food, and beverages. 100.77...) Exceptions to Contributions § 100.77 Invitations, food, and beverages. The cost of invitations, food and... invitations, food and beverages provided by the individual on behalf of the candidate does not exceed $1,000...

  3. 11 CFR 100.137 - Invitations, food, and beverages.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 11 Federal Elections 1 2013-01-01 2012-01-01 true Invitations, food, and beverages. 100.137...) Exceptions to Expenditures § 100.137 Invitations, food, and beverages. The cost of invitations, food, and... invitations, food and beverages provided by the individual on behalf of the candidate does not exceed $1,000...

  4. 11 CFR 100.77 - Invitations, food, and beverages.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 11 Federal Elections 1 2011-01-01 2011-01-01 false Invitations, food, and beverages. 100.77...) Exceptions to Contributions § 100.77 Invitations, food, and beverages. The cost of invitations, food and... invitations, food and beverages provided by the individual on behalf of the candidate does not exceed $1,000...

  5. 11 CFR 100.137 - Invitations, food, and beverages.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 11 Federal Elections 1 2011-01-01 2011-01-01 false Invitations, food, and beverages. 100.137...) Exceptions to Expenditures § 100.137 Invitations, food, and beverages. The cost of invitations, food, and... invitations, food and beverages provided by the individual on behalf of the candidate does not exceed $1,000...

  6. An expert protocol for immunofluorescent detection of calcium channels in tsA-201 cells.

    PubMed

    Koch, Peter; Herzig, Stefan; Matthes, Jan

    Pore-forming subunits of voltage gated calcium channels (VGCC) are large membrane proteins (260kDa) containing 24 transmembrane domains. Despite transfection with viral promoter driven vectors, biochemical analysis of VGCC is often hampered by rather low expression levels in heterologous systems rendering VGCC challenging targets. Especially in immunofluorescent detection, calcium channels are demanding proteins. We provide an expert step-by-step protocol with adapted conditions for handling procedures (tsA-201 cell culture, transient transfection, incubation time and temperature at 28°C or 37°C and immunostaining) to address the L-type calcium-channel pore Ca v 1.2 in an immunofluorescent approach. We performed immunocytochemical analysis of Ca v 1.2 expression at single-cell level in combination with detection of different markers for cellular organelles. We show confluency levels and shapes of tsA-201 cells at different time points during an experiment. Our experiments reveal sufficient levels of Ca v 1.2 protein and a correct Ca v 1.2 expression pattern in polygonal shaped cells already 12h after transfection. A sequence of elaborated protocol modifications allows subcellular localization analysis of Ca v 1.2 in an immunocytochemical approach. We provide a protocol that may be used to achieve insights into physiological and pathophysiological processes involving voltage gated calcium channels. Our protocol may be used for expression analysis of other challenging proteins and efficient overexpression may be exploited in related biochemical techniques requiring immunolabels. Copyright © 2016 Elsevier Inc. All rights reserved.

  7. 11 CFR 100.77 - Invitations, food, and beverages.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 11 Federal Elections 1 2013-01-01 2012-01-01 true Invitations, food, and beverages. 100.77 Section...) Exceptions to Contributions § 100.77 Invitations, food, and beverages. The cost of invitations, food and... invitations, food and beverages provided by the individual on behalf of the candidate does not exceed $1,000...

  8. The Invitational Imagination for Theory, Research, and Practice.

    ERIC Educational Resources Information Center

    Novak, John M.

    This paper argues that just as imagination has been important for the inception and promotion of invitational education, it is also necessary for the development of inviting research strategies. Applying the educative process to the study of inviting, recommendations are made for relating the constituent parts of the inviting stance (optimism,…

  9. The Induction Effect of Am80 and TSA on ESC Differentiation via Regulation of Stra8 in Chicken.

    PubMed

    Zhang, Yani; Zuo, Qisheng; Liu, Zhiyong; Li, Dong; Tang, Beibei; Xiao, Tian-Rong; Lian, Chao; Wang, Yingjie; Jin, Kai; Wang, Yilin; Zhang, Wenhui; Li, Bichun

    2015-01-01

    Stra8 encodes stimulated by retinoic acid gene 8, a protein that is important for initiation of meiosis in mammals and birds. This study was aimed at identifying the active control area of chicken STRA8 gene core promoter, to screen optimum inducers of the STRA8 gene, thus to enhance the differentiation of embryonic stem cells (ESCs) into spermatogonial stem cells. Fragments of chicken STRA8 gene promoter were cloned into fluorescent reporter plasmids and transfected into DF-1 cells. Then Dual-Luciferase® Reporter Assay System was used to identify the activity of the STRA8 gene under different inducers. Our studies showed that the promoter fragment -1055 bp to +54 bp of Suqin chicken Stra8 revealed the strongest activity. The dual-luciferase® reporter showed that Tamibarotene (Am80) and TrichostatinA (TSA) could significantly enhance STRA8 transcription. The in vitro inductive culture of chicken ESCs demonstrated that spermatogonial stem cells (SSC)-like cells appeared and Integrinβ1 protein was expressed on day 10, indicating that Am80 and TSA can promote ESCs differentiation into SSCs via regulation of Stra8.

  10. The Induction Effect of Am80 and TSA on ESC Differentiation via Regulation of Stra8 in Chicken

    PubMed Central

    Zhang, Yani; Zuo, Qisheng; Liu, Zhiyong; Li, Dong; Tang, Beibei; Xiao, Tian-rong; Lian, Chao; Wang, Yingjie; Jin, Kai; Wang, Yilin; Zhang, Wenhui; Li, Bichun

    2015-01-01

    Stra8 encodes stimulated by retinoic acid gene 8, a protein that is important for initiation of meiosis in mammals and birds. This study was aimed at identifying the active control area of chicken STRA8 gene core promoter, to screen optimum inducers of the STRA8 gene, thus to enhance the differentiation of embryonic stem cells (ESCs) into spermatogonial stem cells. Fragments of chicken STRA8 gene promoter were cloned into fluorescent reporter plasmids and transfected into DF-1 cells. Then Dual-Luciferase® Reporter Assay System was used to identify the activity of the STRA8 gene under different inducers. Our studies showed that the promoter fragment −1055 bp to +54 bp of Suqin chicken Stra8 revealed the strongest activity. The dual-luciferase® reporter showed that Tamibarotene (Am80) and TrichostatinA (TSA) could significantly enhance STRA8 transcription. The in vitro inductive culture of chicken ESCs demonstrated that spermatogonial stem cells (SSC)-like cells appeared and Integrinβ1 protein was expressed on day 10, indicating that Am80 and TSA can promote ESCs differentiation into SSCs via regulation of Stra8. PMID:26606052

  11. 49 CFR 1503.421 - Streamlined civil penalty procedures for certain security violations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... PROCEDURAL RULES INVESTIGATIVE AND ENFORCEMENT PROCEDURES Assessment of Civil Penalties by TSA § 1503.421 Streamlined civil penalty procedures for certain security violations. (a) Notice of violation. TSA, at the... violations described in the section and as otherwise provided by the Administrator. TSA may serve a Notice of...

  12. 75 FR 12514 - Stainless Steel Bar From Brazil: Preliminary Results of Antidumping Duty Administrative Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-16

    ... Brazil: Preliminary Results of Antidumping Duty Administrative Review AGENCY: Import Administration... results are adopted in our final results of administrative review, we will instruct U.S. Customs and... invited to comment on these preliminary results of review. We intend to issue the final results of review...

  13. Antiproliferative effects of TSA, PXD‑101 and MS‑275 in A2780 and MCF7 cells: Acetylated histone H4 and acetylated tubulin as markers for HDACi potency and selectivity.

    PubMed

    Androutsopoulos, Vasilis P; Spandidos, Demetrios A

    2017-12-01

    Inhibition of histone deacetylase enzymes (HDACs) has been well documented as an attractive target for the development of chemotherapeutic drugs. The present study investigated the effects of two prototype hydroxamic acid HDAC inhibitors, namely Trichostatin A (TSA) and Belinostat (PXD‑101) and the benzamide Entinostat (MS‑275) in A2780 ovarian carcinoma and MCF7 breast adenocarcinoma cells. The three HDACi inhibited the proliferation of A2780 and MCF7 cells at comparable levels, below the µM range. Enzyme inhibition assays in a cell‑free system showed that TSA was the most potent inhibitor of total HDAC enzyme activity followed by PXD‑101 and MS‑275. Incubation of A2780 and MCF7 cells with the hydroxamates TSA and PXD‑101 for 24 h resulted in a dramatic increase of acetylated tubulin induction (up to 30‑fold for TSA). In contrast to acetylated tubulin, western blot analysis and flow cytometry indicated that the induction of acetylated histone H4 was considerably smaller. The benzamide MS‑275 exhibited nearly a 2‑fold induction of acetylated histone H4 and an even smaller induction of acetylated tubulin in A2780 and MCF7 cells. Taken together, these data suggest that although the three HDACi were equipotent in inhibiting proliferation of MCF7 and A2780 cells, only the benzamide MS‑275 did not induce acetylated tubulin expression, a marker of class IIb HDACs.

  14. HDAC gene expression in pancreatic tumor cell lines following treatment with the HDAC inhibitors panobinostat (LBH589) and trichostatine (TSA).

    PubMed

    Mehdi, Ouaïssi; Françoise, Silvy; Sofia, Costa Lima; Urs, Giger; Kevin, Zemmour; Bernard, Sastre; Igor, Sielezneff; Anabela, Cordeiro-da-Silva; Dominique, Lombardo; Eric, Mas; Ali, Ouaïssi

    2012-01-01

    In this study, the effect of LBH589 and trichostatin (TSA), a standard histone deacetylase inhibitor (HDACi) toward the growth of pancreatic cancer cell lines was studied. Thus, we examined for the first time, the HDAC family gene expression levels before and after drug treatment. Several human pancreatic cancer cell lines (Panc-1, BxPC-3, SOJ-6) and a normal human pancreatic duct immortalized epithelial cell line (HPDE/E6E7) were used as target cells. The cell growth was measured by MTT assay, cell cycle alteration, membrane phosphatidylserine exposure, DNA fragmentation, mitochondrial membrane potential loss, RT-PCR and Western blots were done using standard methods. The effect of drugs on tumor growth in vivo was studied using subcutaneous xenograft model. Except in the case of certain HDAC gene/tumor cell line couples: (SIRT1/HPDE-SOJ6/TSA- or LBH589-treated cells; LBH589-treated Panc-1 Cells; HDAC2/BxPC-3/LBH589-treated cells or TSA-treated SOJ-6-1 cells), there were no major significant changes of HDACs genes transcription in cells upon drug treatment. However, significant variation in HDACs and SIRTs protein expression levels could be seen among individual cell samples. The in vivo results showed that LBH589 formulation exhibited similar tumor reduction efficacy as the commercial drug gemcitabine. Our data demonstrate that LBH589 induced the death of pancreatic tumor cell by apoptosis. In line with its in vitro activity, LBH589 achieved a significant reduction in tumor growth in BxPC-3 pancreatic tumor cell line subcutaneous xenograft mouse model. Furthermore, exploring the impact of LBH589 on HDACs encoding genes expression revealed for the first time that some of them, depending on the cell line considered, seem to be regulated during translation. Copyright © 2012 IAP and EPC. Published by Elsevier B.V. All rights reserved.

  15. Administrative Narcissism and the Tyranny of Isolation: Its Decline and Fall, 1954-1984.

    ERIC Educational Resources Information Center

    Walker, W. G.

    1984-01-01

    An invited perspective article relects on developments related to the professorship of educational administration in the United States. The originally Americocentric approach is now in decline as leaders seek to learn from abroad, widen theory bases, and observe new modes of administrator preparation. (Author/MLF)

  16. 78 FR 55274 - Privacy Act of 1974; Department of Homeland Security/Transportation Security Administration-DHS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-10

    ... enforcement, immigration, and intelligence databases, including a fingerprint-based criminal history records... boarding pass printing instruction. If the passenger's identifying information matches the entry on the TSA... enforcement, immigration, intelligence, or other homeland security functions. In addition, TSA may share...

  17. Cross-Cultural Equivalence and Psychometric Properties of the Traditional Chinese Version of the Inviting School Survey-Revised

    ERIC Educational Resources Information Center

    Smith, Kenneth H.

    2011-01-01

    The Inviting School Survey-Revised (ISS-R) was adapted and translated into Traditional Chinese (ISS-RC), using a five-step process, based on international test administration guidelines, involving judgmental, logical, and empirical methods. Both versions were administered to a convenience sample of Chinese-English fluent Hong Kong school community…

  18. 78 FR 25530 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel HOWLIN AT THE MOON...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-01

    ... DEPARTMENT OF TRANSPORTATION Maritime Administration [Docket No. MARAD-2013 0050] Requested Administrative Waiver of the Coastwise Trade Laws: Vessel HOWLIN AT THE MOON; Invitation for Public Comments... HOWLIN AT THE MOON is: [[Page 25531

  19. Inviting Confidence in School: Invitations as a Critical Source of the Academic Self-Efficacy Beliefs of Entering Middle School Students

    ERIC Educational Resources Information Center

    Usher, Ellen L.; Pajares, Frank

    2006-01-01

    The purpose of this study was to examine whether constructs drawn from invitational theory serve as additional sources of self-efficacy beliefs of students in Grade 6 (N = 468). The hypothesized sources and the invitational constructs each correlated with academic self-efficacy. Invitations, mastery experience, and physiological state predicted…

  20. Loss of Vacuolar H+-ATPase (V-ATPase) Activity in Yeast Generates an Iron Deprivation Signal That Is Moderated by Induction of the Peroxiredoxin TSA2 *

    PubMed Central

    Diab, Heba I.; Kane, Patricia M.

    2013-01-01

    Vacuolar H+-ATPases (V-ATPases) acidify intracellular organelles and help to regulate overall cellular pH. Yeast vma mutants lack V-ATPase activity and allow exploration of connections between cellular pH, iron, and redox homeostasis common to all eukaryotes. A previous microarray study in a vma mutant demonstrated up-regulation of multiple iron uptake genes under control of Aft1p (the iron regulon) and only one antioxidant gene, the peroxiredoxin TSA2 (Milgrom, E., Diab, H., Middleton, F., and Kane, P. M. (2007) Loss of vacuolar proton-translocating ATPase activity in yeast results in chronic oxidative stress. J. Biol. Chem. 282, 7125–7136). Fluorescent biosensors placing GFP under transcriptional control of either an Aft1-dependent promoter (PFIT2-GFP) or the TSA2 promoter (PTSA2-GFP) were constructed to monitor transcriptional signaling. Both biosensors were up-regulated in the vma2Δ mutant, and acute V-ATPase inhibition with concanamycin A induced coordinate up-regulation from both promoters. PTSA2-GFP induction was Yap1p-dependent, indicating an oxidative stress signal. Total cell iron measurements indicate that the vma2Δ mutant is iron-replete, despite up-regulation of the iron regulon. Acetic acid up-regulated PFIT2-GFP expression in wild-type cells, suggesting that loss of pH control contributes to an iron deficiency signal in the mutant. Iron supplementation significantly decreased PFIT2-GFP expression and, surprisingly, restored PTSA2-GFP to wild-type levels. A tsa2Δ mutation induced both nuclear localization of Aft1p and PFIT2-GFP expression. The data suggest a novel function for Tsa2p as a negative regulator of Aft1p-driven transcription, which is induced in V-ATPase mutants to limit transcription of the iron regulon. This represents a new mechanism bridging the antioxidant and iron-regulatory pathways that is intimately linked to pH homeostasis. PMID:23457300

  1. 75 FR 25311 - Requested Administrative Waiver of the Coastwise Trade Laws

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-07

    .... ACTION: Invitation for public comments on a requested administrative waiver of the Coastwise Trade Laws... Gurland, Secretary, Maritime Adminstration. [FR Doc. 2010-10903 Filed 5-6-10; 8:45 am] BILLING CODE 4910...

  2. Looking for the Inviting and Just School Environment.

    ERIC Educational Resources Information Center

    Bennett, A. C.; Novak, J. M.

    This paper explores the relationship between invitational and moral education. Invitational education is defined as a system which intentionally invites students to perceive themselves as valuable, able, and responsible and to behave accordingly. Moral education is a process based on Lawrence Kohlberg's levels of moral reasoning which advocates…

  3. 40 CFR 1503.1 - Inviting comments.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... clearinghouse, for securing State and local reviews of the draft environmental impact statements. (3) Request... 40 Protection of Environment 33 2011-07-01 2011-07-01 false Inviting comments. 1503.1 Section 1503.1 Protection of Environment COUNCIL ON ENVIRONMENTAL QUALITY COMMENTING § 1503.1 Inviting comments...

  4. 40 CFR 1503.1 - Inviting comments.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... clearinghouse, for securing State and local reviews of the draft environmental impact statements. (3) Request... 40 Protection of Environment 32 2010-07-01 2010-07-01 false Inviting comments. 1503.1 Section 1503.1 Protection of Environment COUNCIL ON ENVIRONMENTAL QUALITY COMMENTING § 1503.1 Inviting comments...

  5. Nurses' attitude and intention of medication administration error reporting.

    PubMed

    Hung, Chang-Chiao; Chu, Tsui-Ping; Lee, Bih-O; Hsiao, Chia-Chi

    2016-02-01

    The Aims of this study were to explore the effects of nurses' attitudes and intentions regarding medication administration error reporting on actual reporting behaviours. Underreporting of medication errors is still a common occurrence. Whether attitude and intention towards medication administration error reporting connect to actual reporting behaviours remain unclear. This study used a cross-sectional design with self-administered questionnaires, and the theory of planned behaviour was used as the framework for this study. A total of 596 staff nurses who worked in general wards and intensive care units in a hospital were invited to participate in this study. The researchers used the instruments measuring nurses' attitude, nurse managers' and co-workers' attitude, report control, and nurses' intention to predict nurses' actual reporting behaviours. Data were collected from September-November 2013. Path analyses were used to examine the hypothesized model. Of the 596 nurses invited to participate, 548 (92%) completed and returned a valid questionnaire. The findings indicated that nurse managers' and co-workers' attitudes are predictors for nurses' attitudes towards medication administration error reporting. Nurses' attitudes also influenced their intention to report medication administration errors; however, no connection was found between intention and actual reporting behaviour. The findings reflected links among colleague perspectives, nurses' attitudes, and intention to report medication administration errors. The researchers suggest that hospitals should increase nurses' awareness and recognition of error occurrence. Regardless of nurse managers' and co-workers' attitudes towards medication administration error reporting, nurses are likely to report medication administration errors if they detect them. Management of medication administration errors should focus on increasing nurses' awareness and recognition of error occurrence. © 2015 John Wiley & Sons Ltd.

  6. The HDAC Inhibitor TSA Ameliorates a Zebrafish Model of Duchenne Muscular Dystrophy.

    PubMed

    Johnson, Nathan M; Farr, Gist H; Maves, Lisa

    2013-09-17

    Zebrafish are an excellent model for Duchenne muscular dystrophy. In particular, zebrafish provide a system for rapid, easy, and low-cost screening of small molecules that can ameliorate muscle damage in dystrophic larvae. Here we identify an optimal anti-sense morpholino cocktail that robustly knocks down zebrafish Dystrophin (dmd-MO). We use two approaches, muscle birefringence and muscle actin expression, to quantify muscle damage and show that the dmd-MO dystrophic phenotype closely resembles the zebrafish dmd mutant phenotype. We then show that the histone deacetylase (HDAC) inhibitor TSA, which has been shown to ameliorate the mdx mouse Duchenne model, can rescue muscle fiber damage in both dmd-MO and dmd mutant larvae. Our study identifies optimal morpholino and phenotypic scoring approaches for dystrophic zebrafish, further enhancing the zebrafish dmd model for rapid and cost-effective small molecule screening.

  7. Fewer invited talks by women in evolutionary biology symposia.

    PubMed

    Schroeder, J; Dugdale, H L; Radersma, R; Hinsch, M; Buehler, D M; Saul, J; Porter, L; Liker, A; De Cauwer, I; Johnson, P J; Santure, A W; Griffin, A S; Bolund, E; Ross, L; Webb, T J; Feulner, P G D; Winney, I; Szulkin, M; Komdeur, J; Versteegh, M A; Hemelrijk, C K; Svensson, E I; Edwards, H; Karlsson, M; West, S A; Barrett, E L B; Richardson, D S; van den Brink, V; Wimpenny, J H; Ellwood, S A; Rees, M; Matson, K D; Charmantier, A; Dos Remedios, N; Schneider, N A; Teplitsky, C; Laurance, W F; Butlin, R K; Horrocks, N P C

    2013-09-01

    Lower visibility of female scientists, compared to male scientists, is a potential reason for the under-representation of women among senior academic ranks. Visibility in the scientific community stems partly from presenting research as an invited speaker at organized meetings. We analysed the sex ratio of presenters at the European Society for Evolutionary Biology (ESEB) Congress 2011, where all abstract submissions were accepted for presentation. Women were under-represented among invited speakers at symposia (15% women) compared to all presenters (46%), regular oral presenters (41%) and plenary speakers (25%). At the ESEB congresses in 2001-2011, 9-23% of invited speakers were women. This under-representation of women is partly attributable to a larger proportion of women, than men, declining invitations: in 2011, 50% of women declined an invitation to speak compared to 26% of men. We expect invited speakers to be scientists from top ranked institutions or authors of recent papers in high-impact journals. Considering all invited speakers (including declined invitations), 23% were women. This was lower than the baseline sex ratios of early-mid career stage scientists, but was similar to senior scientists and authors that have published in high-impact journals. High-quality science by women therefore has low exposure at international meetings, which will constrain Evolutionary Biology from reaching its full potential. We wish to highlight the wider implications of turning down invitations to speak, and encourage conference organizers to implement steps to increase acceptance rates of invited talks. © 2013 The Authors. Journal of Evolutionary Biology © 2013 European Society For Evolutionary Biology.

  8. Fewer invited talks by women in evolutionary biology symposia

    PubMed Central

    Schroeder, J; Dugdale, H L; Radersma, R; Hinsch, M; Buehler, D M; Saul, J; Porter, L; Liker, A; De Cauwer, I; Johnson, P J; Santure, A W; Griffin, A S; Bolund, E; Ross, L; Webb, T J; Feulner, P G D; Winney, I; Szulkin, M; Komdeur, J; Versteegh, M A; Hemelrijk, C K; Svensson, E I; Edwards, H; Karlsson, M; West, S A; Barrett, E L B; Richardson, D S; van den Brink, V; Wimpenny, J H; Ellwood, S A; Rees, M; Matson, K D; Charmantier, A; dos Remedios, N; Schneider, N A; Teplitsky, C; Laurance, W F; Butlin, R K; Horrocks, N P C

    2013-01-01

    Lower visibility of female scientists, compared to male scientists, is a potential reason for the under-representation of women among senior academic ranks. Visibility in the scientific community stems partly from presenting research as an invited speaker at organized meetings. We analysed the sex ratio of presenters at the European Society for Evolutionary Biology (ESEB) Congress 2011, where all abstract submissions were accepted for presentation. Women were under-represented among invited speakers at symposia (15% women) compared to all presenters (46%), regular oral presenters (41%) and plenary speakers (25%). At the ESEB congresses in 2001–2011, 9–23% of invited speakers were women. This under-representation of women is partly attributable to a larger proportion of women, than men, declining invitations: in 2011, 50% of women declined an invitation to speak compared to 26% of men. We expect invited speakers to be scientists from top ranked institutions or authors of recent papers in high-impact journals. Considering all invited speakers (including declined invitations), 23% were women. This was lower than the baseline sex ratios of early-mid career stage scientists, but was similar to senior scientists and authors that have published in high-impact journals. High-quality science by women therefore has low exposure at international meetings, which will constrain Evolutionary Biology from reaching its full potential. We wish to highlight the wider implications of turning down invitations to speak, and encourage conference organizers to implement steps to increase acceptance rates of invited talks. PMID:23786459

  9. Draft genome sequence of multidrug-resistant Staphylococcus haemolyticus IPK_TSA25 harbouring a Staphylococcus aureus plasmid, pS0385-1.

    PubMed

    Kim, Hyung Jun; Jang, Soojin

    2017-12-01

    Staphylococcus haemolyticus is the second most frequently isolated coagulase-negative staphylococci from blood cultures. Moreover, multidrug resistance associated with the genome flexibility of S. haemolyticus has been increasingly reported worldwide. Here we report the draft genome sequence of multidrug-resistant S. haemolyticus IPK_TSA25 isolated from a building surface in South Korea. Genomic DNA of S. haemolyticus IPK_TSA25 was sequenced using the PacBio RS II sequencing platform. Generated reads were assembled using PacBio SMRT Analysis 2.3.0. The draft genome was annotated and antibiotic resistance genes were identified. The genome of 2517398bp contains various antibiotic resistance genes associated with resistance to β-lactams, aminoglycosides and macrolides. Genome analysis also revealed chromosomal integration of the full-length Staphylococcus aureus plasmid pS0385-1 containing a tetracycline resistance gene. The genome sequence reported in this study will provide valuable information to understand the flexibility of the S. haemolyticus genome, which facilitates acquisition of antibiotic resistance genes and contributes to the dissemination of antibiotic resistance by this emerging pathogen. Copyright © 2017 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.

  10. Pluripotency maintenance in mouse somatic cell nuclear transfer embryos and its improvement by treatment with the histone deacetylase inhibitor TSA.

    PubMed

    Hai, Tang; Hao, Jie; Wang, Liu; Jouneau, Alice; Zhou, Qi

    2011-02-01

    Reprogramming of somatic cells to pluripotency can be achieved by nuclear transfer into enucleated oocytes (SCNT). A key event of this process is the demethylation of the Oct4 gene and its temporally and spatially regulated expression. Different studies have shown that it occurs abnormally in some SCNT embryos. TSA is a histone deacetylase inhibitor known to increase the efficiency of development to term of SCNT embryos, but its impact on the developmental features of SCNT embryos is poorly understood. Here, we have followed the fate of the pluripotent cells within SCNT embryos, from the late blastocyst to the early epiblast prior to gastrulation. Our data show a delay in development correlated with a defect in forming and maintaining a correct number of Oct4 expressing ICM and epiblast cells in SCNT embryos. As a consequence, during the outgrowth phase of embryonic stem cell derivation as well as during diapause in vivo, part of the SCNT blastocysts completely lose their ICM cells. Meanwhile, the others display a correctly reprogrammed ICM compatible with the derivation of ES cells and development of the epiblast. Our data also indicate that TSA favors the establishment of pluripotency in SCNT embryos.

  11. Invitational Learning for Counseling and Development.

    ERIC Educational Resources Information Center

    Purkey, William W.; And Others

    This monograph describes the theory and practice of the invitational learning model as it applies to school counseling and development. Invitational learning is a paradigm for personal and professional functioning, and for organizational restructuring, based on four values regarding the nature of people and their potential and the nature of…

  12. Modified g-C3N4/TiO2 nanosheets/ZnO ternary facet coupled heterojunction for photocatalytic degradation of p-toluenesulfonic acid (p-TSA) under visible light

    NASA Astrophysics Data System (ADS)

    Jiang, Dong; Yu, Han; Yu, Hongbing

    2017-01-01

    Novel ternary nanocomposites with facet coupled structure were synthesized by using modified g-C3N4, TiO2 nanosheets and nano-ZnO. Nanosheet/nanosheet heterojunction structure was investigated by TEM, XPS and XRD. FT-IR and Nitrogen adsorption were illustrated for chemical/physical structure analyses. Solution of p-Toluenesulfonic acid (p-TSA) was chosen as target pollutant for visible light photodegradation and the excellent removal efficiency was achieved by this structurally modified g-C3N4/TiO2/ZnO hybrid. The visible light absorption improvement and quantum efficiency enhancement, which were testified by UV-vis DRS, PL and p-TSA photodegradation measurements, due to the facet coupled structure and appropriate quantity of modified g-C3N4 in the nanocomposites.

  13. Letter from Administrator Pruitt on Meeting to Reduce Childhood Lead Exposure

    EPA Pesticide Factsheets

    Administrator Pruitt has formally invited members of the President’s Task Force on Environmental Health Risks and Safety Risks to Children to participate in a meeting to discuss next steps in developing a federal strategy to reduce childhood lead exposure.

  14. Mapping Invitations to Participate: An Investigation in Museum Interpretation

    ERIC Educational Resources Information Center

    Kothe, Elsa Lenz

    2016-01-01

    This a/r/tographic inquiry delves into questions about participatory art museum practice, specifically seeking to understand the nature of invitations to participate. Utilising drawings, writing and mapping of embodied participation, questions of how individuals are invited to participate in various locations and how these invitations inform the…

  15. The Perceived School Climate in Invitational Schools in Hong Kong: Using the Chinese Version of the Inviting School Survey-Revised (ISS-R)

    ERIC Educational Resources Information Center

    Ng, Carmen K. M.; Yuen, Mantak

    2011-01-01

    This article describes the use of the Chinese translation of the revised Inviting School Survey (ISS-R; Smith, 2005; Smith & Bernard, 2004) to measure the invitational climate of seven invitational secondary schools in Hong Kong. The five subscales of Chinese version of ISS-R were found to be valid and reliable in a sample of 706 Grade 11…

  16. 9/11 Commission Recommendations: Implementation Status

    DTIC Science & Technology

    2006-12-04

    that occurred from 2004-2005. U.S., Afghan, and international officials have cited the cultivation and trafficking as a serious strategic threat to U.S...Administration ( TSA ). alien smuggling in certain circumstances and required the Secretary of Homeland Security to develop an outreach program in the...the Transportation Security Administration ( TSA ) take over the function of prescreening passenger names using the larger set of watchlists maintained

  17. Invitational Leadership: Does It Make a Difference?

    ERIC Educational Resources Information Center

    Egley, Robert

    2003-01-01

    This study investigated the relationship between professionally and personally inviting behaviors of high school principals in the state of Mississippi and: (a) Teacher Job Satisfaction, (b) Principal Effectiveness, (c) Principal as an Agent of School Improvement, and (d) Principal's Invitational Quotient, and (e) The Computed Accreditation…

  18. Correlation of serum biomarkers (TSA & LSA) and epithelial dysplasia in early diagnosis of oral precancer and oral cancer.

    PubMed

    Sawhney, Hemant; Kumar, C Anand

    Oral cancer is currently the most frequent cause of cancer-related deaths, which is usually preceded by oral pre-cancerous lesions and conditions. Altered glycosylation of glycoconjugates, such as sialic acid, fucose, etc. are amongst the important molecular changes that accompany malignant transformation. The purpose of our study was to evaluate usefulness of serum Total Sialic Acid (TSA) and serum Lipid-Bound Sialic Acid (LSA) as markers of oral precancerous lesions and histopathologically correlating them with grades of epithelial dysplasia. Blood samples were collected from 50 patients with oral precancer (Leukoplakia & OSMF), 25 patients with untreated oral cancer and 25 healthy subjects. Serum sialic acid (total and lipid bound) levels were measured spectrophotometrically. Tissue samples from all the patients were evaluated for dysplasia. Serum levels of total and lipid bound sialic acid were significantly elevated in patients with oral precancer and cancer when compared with healthy subjects. Analysis of variance test documented that there is progressive rise in serum levels of sialic acid with the degree of dysplastic changes in oral precancer patients. We observed positive correlation between serum levels of the markers and the extent of malignant disease (TNM Clinical staging) as well as histopathological grades. The results suggested that serum levels of TSA and LSA progressively increases with grades of dysplasia in precancerous groups and cancer group, when compared with healthy controls. These glycoconjugates, especially LSA has the clinical utility in indicating a premalignant change.

  19. Enhancement of immune response induced by DNA vaccine cocktail expressing complete LACK and TSA genes against Leishmania major.

    PubMed

    Ghaffarifar, Fatemeh; Jorjani, Ogholniaz; Sharifi, Zohreh; Dalimi, Abdolhossein; Hassan, Zuhair M; Tabatabaie, Fatemeh; Khoshzaban, Fariba; Hezarjaribi, Hajar Ziaei

    2013-04-01

    Leishmaniasis is an important disease in humans. Leishmania homologue of receptor for Activated C Kinase (LACK) and thiol specific antioxidant (TSA) as immuno-dominant antigens of Leishmania major are considered the most promising molecules for a DNA vaccine. We constructed a DNA cocktail, containing plasmids encoding LACK and TSA genes of Leishmania major and evaluated the immune response and survival rate in BALB/c mice. IgG and Interferon gamma values were noticeably increased in the immunized group with DNA cocktail vaccine, which were significantly higher than those in the single-gene vaccinated and control groups (p < 0.05) following the immunization and after challenging with Leishmania major. Interleukin 4 values were decreased in all immunized groups, but only in DNA vaccine cocktail and single-gene vaccination with pc-LACK there were statistical differences with control groups (p > 0.05). The immunized mice with the cocktail DNA vaccine presented a considerable reduction in diameter of lesion compared to other groups and a significant difference was observed (p < 0.05) in this regard. The survival time of the immunized mice with the cocktail DNA vaccine was significantly higher than that in the other groups (p < 0.05) after their being challenged with Leishmania major. The findings of this study indicated that the cocktail DNA vaccine increased the cellular response and survival rate and induced protection against infection with Leishmania in the mice. © 2012 The Authors © 2012 APMIS.

  20. 49 CFR 1542.303 - Security Directives and Information Circulars.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY AIRPORT SECURITY... Information Circular to notify airport operators of security concerns. When TSA determines that additional... aviation, TSA issues a Security Directive setting forth mandatory measures. (b) Each airport operator must...

  1. 49 CFR 1542.303 - Security Directives and Information Circulars.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY AIRPORT SECURITY... Information Circular to notify airport operators of security concerns. When TSA determines that additional... aviation, TSA issues a Security Directive setting forth mandatory measures. (b) Each airport operator must...

  2. 49 CFR 1542.303 - Security Directives and Information Circulars.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY AIRPORT SECURITY... Information Circular to notify airport operators of security concerns. When TSA determines that additional... aviation, TSA issues a Security Directive setting forth mandatory measures. (b) Each airport operator must...

  3. 49 CFR 1542.303 - Security Directives and Information Circulars.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY AIRPORT SECURITY... Information Circular to notify airport operators of security concerns. When TSA determines that additional... aviation, TSA issues a Security Directive setting forth mandatory measures. (b) Each airport operator must...

  4. Inviting Research: Paradigms and Projects for a Theory of Educational Practice.

    ERIC Educational Resources Information Center

    Novak, John M.

    Research projects needed in invitational education will vary according to the concept of invitational education held. Three paradigms for invitational education are presented, with research projects suggested for each. First, for those who see invitational education as an "integrative setting," it is suggested that the development of…

  5. Therapeutic potential of trichostatin A to control inflammatory and fibrogenic disorders of the ocular surface.

    PubMed

    Kitano, Ai; Okada, Yuka; Yamanka, Osamu; Shirai, Kumi; Mohan, Rajiv R; Saika, Shizuya

    2010-12-31

    To examine the effects of a histone deacetylase inhibitor, Trichostatin A (TSA), on the behavior of macrophages and subconjunctival fibroblasts in vitro and on ocular surface inflammation and scarring in vivo using an alkali burn wound healing model. Effects of TSA on expression of inflammation-related growth factors or collagen I were examined by real-time RT-PCR or immunoassay in mouse macrophages or human subconjunctival fibroblasts. Effects of TSA on trans forming growth factor β (TGFβ)/Smad signaling were evaluated with western blotting and/or immunocytochemistry. Alkali-burn injuries on the eyes of mice were performed with three µl of 0.5 N NaOH under general and topical anesthesia. TSA (600 µg/Kg daily) or vehicle was administered to animals via intraperitoneal (i.p.) injection. Histology and real-time RT-PCR investigations evaluated the effects of TSA on the healing process of the cornea. TSA inhibited TGFβ 1 and vascular endothelial growth factor (VEGF) expression in macrophages, and TGFβ1 and collagen I in ocular fibroblasts. It elevated the expression of 5'-TG-3'-interacting factor (TGIF) and Smad7 in fibroblasts and blocked nuclear translocation of phospho-Smad2. Real-time PCR and immunocytochemistry studies showed that systemic administration of TSA suppressed the inflammation and fibrotic response in the stroma and accelerated epithelial healing in the alkali-burned mouse cornea. Systemic administration of TSA reduces inflammatory and fibrotic responses in the alkali-burned mouse ocular surface in vivo. The mechanisms of action involve attenuation of Smad signal in mesenchymal cells and reduction in the activation and recruitment of macrophages. TSA has the potential to treat corneal scarring in vivo.

  6. Autologous hematopoietic progenitor cell mobilization and collection in adult patients presenting with multiple myeloma and lymphoma: A position-statement from the Turkish Society of Apheresis (TSA).

    PubMed

    Tekgündüz, Emre; Arat, Mutlu; Göker, Hakan; Özdoğu, Hakan; Kaynar, Leylagül; Çağırgan, Seçkin; Erkurt, Mehmet Ali; Vural, Filiz; Kiki, İlhami; Altuntaş, Fevzi; Demirkan, Fatih

    2017-12-01

    Autologous hematopoietic cell transplantation (AHCT) is a routinely used procedure in the treatment of adult patients presenting with multiple myeloma (MM), Hodgkin lymphoma (HL) and various subtypes of non-Hodgkin lymphoma (NHL) in upfront and relapsed/refractory settings. Successful hematopoietic progenitor cell mobilization (HPCM) and collection are the rate limiting first steps for application of AHCT. In 2015, almost 1700 AHCT procedures have been performed for MM, HL and NHL in Turkey. Although there are recently published consensus guidelines addressing critical issues regarding autologous HPCM, there is a tremendous heterogeneity in terms of mobilization strategies of transplant centers across the world. In order to pave the way to a more standardized HPCM approach in Turkey, Turkish Society of Apheresis (TSA) assembled a working group consisting of experts in the field. Here we report the position statement of TSA regarding autologous HPCM mobilization strategies in adult patients presenting with MM and lymphoma. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. 49 CFR 1542.109 - Alternate means of compliance.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY AIRPORT SECURITY Airport Security Program § 1542.109 Alternate means of compliance. If in TSA's judgment, the overall safety and security of the airport, and aircraft operator or foreign air carrier operations are not diminished, TSA...

  8. 49 CFR 1542.109 - Alternate means of compliance.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY AIRPORT SECURITY Airport Security Program § 1542.109 Alternate means of compliance. If in TSA's judgment, the overall safety and security of the airport, and aircraft operator or foreign air carrier operations are not diminished, TSA...

  9. 49 CFR 1542.109 - Alternate means of compliance.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY AIRPORT SECURITY Airport Security Program § 1542.109 Alternate means of compliance. If in TSA's judgment, the overall safety and security of the airport, and aircraft operator or foreign air carrier operations are not diminished, TSA...

  10. 49 CFR 1542.109 - Alternate means of compliance.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY AIRPORT SECURITY Airport Security Program § 1542.109 Alternate means of compliance. If in TSA's judgment, the overall safety and security of the airport, and aircraft operator or foreign air carrier operations are not diminished, TSA...

  11. 49 CFR 1542.109 - Alternate means of compliance.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY AIRPORT SECURITY Airport Security Program § 1542.109 Alternate means of compliance. If in TSA's judgment, the overall safety and security of the airport, and aircraft operator or foreign air carrier operations are not diminished, TSA...

  12. 78 FR 11216 - Enforcement Actions Summary

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-15

    ...] Enforcement Actions Summary AGENCY: Transportation Security Administration, DHS. ACTION: Notice of... an annual summary of all enforcement actions taken by TSA under the authority granted in the... public with an annual summary of all enforcement actions taken by TSA under this subsection; and include...

  13. 77 FR 12865 - Enforcement Actions Summary

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-02

    ...] Enforcement Actions Summary AGENCY: Transportation Security Administration, DHS. ACTION: Notice of... an annual summary of all enforcement actions taken by TSA under the authority granted in the... public with an annual summary of all enforcement actions taken by TSA under this subsection; and include...

  14. 76 FR 9357 - Enforcement Actions Summary

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-17

    ...] Enforcement Actions Summary AGENCY: Transportation Security Administration, DHS. ACTION: Notice of... an annual summary of all enforcement actions taken by TSA under the authority granted in the... to provide the public with an annual summary of all enforcement actions taken by TSA under this...

  15. The Basic Tenets of Invitational Theory and Practice: An Invitational Glossary

    ERIC Educational Resources Information Center

    Shaw, Daniel E.; Siegel, Betty L.; Schoenlein, Allyson

    2013-01-01

    A review of the literature which concentrates on Invitational Theory and Practice (ITP) has revealed an inconsistent and oft times confusing or contradictory use of named concepts, labels, phrases, wordings, definitions, and other such titles of major ITP principles (Shaw and Siegel, 2010). Presented in a glossary type format, the purpose of this…

  16. Schools without Fear. Proceedings of the Annual International Alliance for Invitational Education Conference (14th). International Alliance for Invitational Education.

    ERIC Educational Resources Information Center

    Francis, Adrianna Hayes, Ed.

    Papers presented at the fourteenth Annual Conference of the Alliance for Invitational Education are (1) "Caring, Sharing, Daring: Three Tests to Help Develop More Inviting Policies, Programmes, and Procedures" (M. Ayers); (2) "Project: Gentlemen on the Move - Combating the Poor Academic and Social Performance of African American Male Youth" (D. F.…

  17. 77 FR 70792 - Privacy Act of 1974; Retirement of Department of Homeland Security Transportation Security...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-27

    ... from its inventory of record systems. TSA will rely upon DHS/ALL-017 General Legal Records (November 23, 2011, 76 FR 72428) to cover its legal activities. Eliminating the system of records notice DHS/TSA-009... Department of Homeland Security Transportation Security Administration System of Records AGENCY: Privacy...

  18. "Invitations" to Character

    ERIC Educational Resources Information Center

    Milliren, Al; Messer, Mitchell H.

    2009-01-01

    If we are to help children develop character, we have to go beyond offering a character education class once or twice each month. We actually have to validate character when we see it or hear about it happening. Through the use of a new strategy called "focused invitations," counselors and teachers can focus on the 36 core components of character.…

  19. Combination of HDAC inhibitor TSA and silibinin induces cell cycle arrest and apoptosis by targeting survivin and cyclinB1/Cdk1 in pancreatic cancer cells.

    PubMed

    Feng, Wan; Cai, Dawei; Zhang, Bin; Lou, Guochun; Zou, Xiaoping

    2015-08-01

    Histone deacetylases (HDAC) are involved in diverse biological processes and therefore emerge as potential targets for pancreatic cancer. Silibinin, an active component of silymarin, is known to inhibit growth of pancreatic cancer in vivo and in vitro. Herein, we examined the cytotoxic effects of TSA in combination with silibinin and investigated the possible mechanism in two pancreatic cancer cell lines (Panc1 and Capan2). Our study found that combination treatment of HDAC inhibitor and silibinin exerted additive growth inhibitory effect on pancreatic cancer cell. Annexin V-FITC/PI staining and flow cytometry analysis demonstrated that combination therapy induced G2/M cell cycle arrest and apoptosis in Panc1and Capan2 cells. The induction of apoptosis was further confirmed by evaluating the activation of caspases. Moreover, treatment with TSA and silibinin resulted in a profound reduction in the expression of cyclinA2, cyclinB1/Cdk1 and survivin. Taken together, our study might indicate that the novel combination of HDAC inhibitor and silibinin could offer therapeutic potential against pancreatic cancer. Copyright © 2015. Published by Elsevier Masson SAS.

  20. Do healthcare administrators care less about health?

    PubMed

    Fleenor, C P; Weis, W L

    1983-01-01

    In a recent study conducted by the authors, Seattle-area managers disclosed a high incidence of discrimination against smokers at the hiring point, and expressed strong preferences for further restrictions on smoking in the workplace. Surprisingly, of the six management groups represented in the study, only healthcare administrators were acquiescent toward the presumed rights of smokers as employees. This anomaly, as well as other insights drawn from the research, is discussed in the article. Reader explanations to the apparent contradiction presented here are invited.

  1. Inviting Policy Development: From Public Relations to Public Creations.

    ERIC Educational Resources Information Center

    Webster, Sheila J.; Novak, John M.

    Inviting policy development is an attempt to cordially summon those who are involved and affected by rules, codes, and procedures to understand and participate in the formulation, implementation, and evaluation of policies. This paper first presents criteria for an invitational framework for policy development and then goes on to discuss the…

  2. 41 CFR 60-250.42 - Invitation to self-identify.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 41 Public Contracts and Property Management 1 2013-07-01 2013-07-01 false Invitation to self-identify. 60-250.42 Section 60-250.42 Public Contracts and Property Management Other Provisions Relating to... PROTECTED VETERANS Affirmative Action Program § 60-250.42 Invitation to self-identify. (a) Special disabled...

  3. 41 CFR 60-250.42 - Invitation to self-identify.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true Invitation to self-identify. 60-250.42 Section 60-250.42 Public Contracts and Property Management Other Provisions Relating to... PROTECTED VETERANS Affirmative Action Program § 60-250.42 Invitation to self-identify. (a) Special disabled...

  4. 41 CFR 60-250.42 - Invitation to self-identify.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 41 Public Contracts and Property Management 1 2011-07-01 2009-07-01 true Invitation to self-identify. 60-250.42 Section 60-250.42 Public Contracts and Property Management Other Provisions Relating to... PROTECTED VETERANS Affirmative Action Program § 60-250.42 Invitation to self-identify. (a) Special disabled...

  5. 41 CFR 60-250.42 - Invitation to self-identify.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 41 Public Contracts and Property Management 1 2012-07-01 2009-07-01 true Invitation to self-identify. 60-250.42 Section 60-250.42 Public Contracts and Property Management Other Provisions Relating to... PROTECTED VETERANS Affirmative Action Program § 60-250.42 Invitation to self-identify. (a) Special disabled...

  6. The Incidence and Types of Occupational Role Stress among University Research Administrators

    ERIC Educational Resources Information Center

    Katsapis, Christine C. A.

    2012-01-01

    This study explored the types of stressors prevalent in the self-reports of university research administrators (URAs) and examined whether or not the degree or type of role stress was influenced by: a) the affiliation of their office unit within their institution, or b) their type. Randomly selected members of NCURA were invited via e-mail to…

  7. FY2007 Supplemental Appropriations for Defense, Foreign Affairs, and Other Purposes

    DTIC Science & Technology

    2007-05-02

    73 Liquidation of TSA Contract and Grant Obligations . . . . . . . . . . . . . . . . . . . . . . 76 Ongoing Katrina Recovery...Administration ( TSA ) contract and grant obligations incurred during FY2002 and FY2003. Congress is also considering additions of unrequested funds for...funding shown in brackets) : ! $520 million for Bradley base sustainment ($1.4 billion in bridge); ! $1.6 billion for the Family of Medium Tactical

  8. 77 FR 23228 - Submission for OMB Review; Evaluation of State Vocational Rehabilitation Agency Administration of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-18

    ... Agency Administration of Supported Employment Programs SUMMARY: The Vocational Rehabilitation (VR... results in a substantial impediment to employment, who can benefit from VR services for employment, and who require VR services. DATES: Interested persons are invited to submit comments on or before May 18...

  9. The Psychometric Properties of the Invitational School Survey (ISS): An Australian Study

    ERIC Educational Resources Information Center

    Smith, Kenneth H.; Barnard, John

    2004-01-01

    This study provides psychometric data on the Inviting School Survey (Purkey & Fuller, 1995) using a rating scale analysis within the framework of the Rasch measurement philosophy (Bond & Fox, 2001; Rasch, 1980). The Inviting School Survey's factor structure and internal consistency are examined and compared with the Invitational Education…

  10. Invitation coverage and participation in Italian cervical, breast and colorectal cancer screening programmes.

    PubMed

    Giorgi Rossi, Paolo; Carrozzi, Giuliano; Federici, Antonio; Mancuso, Pamela; Sampaolo, Letizia; Zappa, Marco

    2018-03-01

    Objectives In Italy, regional governments organize cervical, breast and colorectal cancer screening programmes, but there are difficulties in regularly inviting all the target populations and participation remains low. We analysed the determinants associated with invitation coverage of and participation in these programmes. Methods We used data on screening programmes from annual Ministry of Health surveys, 1999-2012 for cervical, 1999-2011 for breast and 2005-2011 for colorectal cancer. For recent years, we linked these data to the results of the national routine survey on preventive behaviours to evaluate the effect of spontaneous screening at Province level. Invitation and participation relative risk were calculated using Generalized Linear Models. Results There is a strong decreasing trend in invitation coverage and participation in screening programmes from North to South Italy. In metropolitan areas, both invitation coverage (rate ratio 0.35-0.96) and participation (rate ratio 0.63-0.88) are lower. An inverse association exists between spontaneous screening and both screening invitation coverage (1-3% decrease in invitation coverage per 1% spontaneous coverage increase) and participation (2% decrease in participation per 1% spontaneous coverage increase) for the three programmes. High recall rate has a negative effect on invitation coverage in the next round for breast cancer (1% decrease in invitation per 1% recall increase). Conclusions Organizational and cultural changes are needed to better implement cancer screening in southern Italy.

  11. We read spam a lot: prospective cohort study of unsolicited and unwanted academic invitations

    PubMed Central

    Bolland, Mark J; Dalbeth, Nicola; Gamble, Greg; Sadler, Lynn

    2016-01-01

    Objectives To assess the amount, relevance, content, and suppressibility of academic electronic spam invitations to attend conferences or submit manuscripts. Design Prospective cohort study. Setting Email accounts of participating academics. Participants Five intrepid academics and a great many publishers, editors, and conference organisers. Intervention Unsubscribing from sender’s distribution lists. Main outcome measures Number of spam invitations received before, immediately after, and one year after unsubscribing from senders’ distribution lists. The proportion of duplicate invitations was also assessed and the relevance of each invitation graded to the recipient’s research interests. A qualitative assessment of the content of spam invitations was conducted. Results At baseline, recipients received an average of 312 spam invitations each month. Unsubscribing reduced the frequency of the invitations by 39% after one month but by only 19% after one year. Overall, 16% of spam invitations were duplicates and 83% had little or no relevance to the recipients’ research interests. Spam invitations were characterised by inventive language, flattery, and exuberance, and they were sometimes baffling and amusing. Conclusions Academic spam is common, repetitive, often irrelevant, and difficult to avoid or prevent. PMID:27974354

  12. 49 CFR 1540.115 - Threat assessments regarding citizens of the United States holding or applying for FAA...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...: Administrator means the Administrator of the Transportation Security Administration. Assistant Administrator means the Assistant Administrator for Intelligence for TSA. Date of service means— (1) The date of personal delivery in the case of personal service; (2) The mailing date shown on the certificate of service...

  13. 49 CFR 1540.115 - Threat assessments regarding citizens of the United States holding or applying for FAA...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...: Administrator means the Administrator of the Transportation Security Administration. Assistant Administrator means the Assistant Administrator for Intelligence for TSA. Date of service means— (1) The date of personal delivery in the case of personal service; (2) The mailing date shown on the certificate of service...

  14. 49 CFR 1540.115 - Threat assessments regarding citizens of the United States holding or applying for FAA...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...: Administrator means the Administrator of the Transportation Security Administration. Assistant Administrator means the Assistant Administrator for Intelligence for TSA. Date of service means— (1) The date of personal delivery in the case of personal service; (2) The mailing date shown on the certificate of service...

  15. 49 CFR 1540.115 - Threat assessments regarding citizens of the United States holding or applying for FAA...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...: Administrator means the Administrator of the Transportation Security Administration. Assistant Administrator means the Assistant Administrator for Intelligence for TSA. Date of service means— (1) The date of personal delivery in the case of personal service; (2) The mailing date shown on the certificate of service...

  16. 49 CFR 1540.115 - Threat assessments regarding citizens of the United States holding or applying for FAA...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...: Administrator means the Administrator of the Transportation Security Administration. Assistant Administrator means the Assistant Administrator for Intelligence for TSA. Date of service means— (1) The date of personal delivery in the case of personal service; (2) The mailing date shown on the certificate of service...

  17. 77 FR 42752 - New Agency Information Collection Activity Under OMB Review: Generic Clearance for the Collection...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-20

    ... DEPARTMENT OF HOMELAND SECURITY Transportation Security Administration New Agency Information... Service Delivery AGENCY: Transportation Security Administration, DHS. ACTION: 30-day Notice. SUMMARY: This notice announces that the Transportation Security Administration (TSA) has forwarded the new Information...

  18. 77 FR 46409 - Procurement List; Additions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-03

    ... Administration (TSA) Space, Lehigh Valley International Airport, 3311 Airport Rd, Allentown, PA. NPA: Via of the...: Custodial Service. National Oceanic and Atmospheric Administration (NOAA) National Weather Service 32 Dawes...

  19. Invitation strategies and coverage in the population-based cancer screening programmes in the European Union.

    PubMed

    Vale, Diama B; Anttila, Ahti; Ponti, Antonio; Senore, Carlo; Sankaranaryanan, Rengaswamy; Ronco, Guglielmo; Segnan, Nereo; Tomatis, Mariano; Žakelj, Maja P; Elfström, Klara M; Lönnberg, Stefan; Dillner, Joakim; Basu, Partha

    2018-03-21

    The aim of this study was to describe the compliance of the population-based cancer screening programmes in the European Union Member States to the invitation strategies enumerated in the European Guidelines and the impact of such strategies on the invitational coverage. Experts in screening programme monitoring from the respective countries provided data. Coverage by invitation was calculated as the proportion of individuals in the target age range receiving a screening invitation over the total number of annualized eligible population. The invitation strategies of 30 breasts, 25 cervical and 27 colorectal national or regional population-based screening programmes are described. Individual mail invitations are sent by 28 breasts, 20 cervical and 25 colorectal screening programmes. Faecal occult blood test kits are sent by post in 17 of the colorectal cancer screening programmes. The majority of programmes claimed to have a population registry, although some use health insurance data as the database for sending invitations. At least 95% invitation coverage was reached by 16 breast, six cervical and five colorectal screening programmes. Majority of the programmes comply with the invitation strategies enumerated in the European guidelines, although there is still scope for improvements. Coverage by invitation is below the desirable level in many population-based cancer screening programmes in European Union.

  20. 75 FR 9915 - Extension of Agency Information Collection Activity Under OMB Review: Certified Cargo Screening...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-04

    ...This notice announces that the Transportation Security Administration (TSA) has forwarded the Information Collection Request (ICR), OMB control number 1652-0053, abstracted below to the Office of Management and Budget (OMB) for renewal in compliance with the Paperwork Reduction Act. The ICR describes the nature of the information collection and its expected burden. TSA published a Federal Register notice, with a 60-day comment period soliciting comments, of the following collection of information on November 16, 2009, 74 FR 58967. TSA has received no comments. The collections include: (1) Applications from entities that wish to become Certified Cargo Screening Facilities (CCSF) or operate as a TSA-approved validation firm; (2) personal information to allow TSA to conduct security threat assessments on key individuals employed by the CCSFs and validation firms; (3) implementation of a standard security program or submission of a proposed modified security program; (4) information on the amount of cargo screened; (5) recordkeeping requirements for CCSFs and validation firms; and (6) submission of validation reports to TSA. TSA is seeking the renewal of the ICR for the continuation of the program in order to secure passenger aircraft carrying cargo by the deadlines set out in the Implementing Recommendations of the 9/11 Commission Act of 2007.

  1. Young Women's Experiences of Resisting Invitations to Use Illicit Drugs

    ERIC Educational Resources Information Center

    Koehn, Corinne V.; O'Neill, Linda K.

    2011-01-01

    Ten young women were interviewed regarding their experiences of resisting invitations to use illicit drugs. Hermeneutic phenomenology was used to gather and analyze information. One key theme was the motivations that inspired women to refuse drug offers. Young women resisted drug invitations because of their desires to be authentic, protect their…

  2. S.C.I.S. - Sciencing Considered from an Invitational Stance.

    ERIC Educational Resources Information Center

    Wheeler, Alan E.

    This paper focuses on the refinement and articulation of the invitational education concept to science teaching in the classroom, an approach consistent with the underlying philosophy of SCIS. The first section of the paper outlines the nature of invitations and disinvitations and describes the ability of teachers to send formal or informal…

  3. A randomized controlled trial in non-responders from Newcastle upon Tyne invited to return a self-sample for Human Papillomavirus testing versus repeat invitation for cervical screening.

    PubMed

    Cadman, Louise; Wilkes, Scott; Mansour, Diana; Austin, Janet; Ashdown-Barr, Lesley; Edwards, Rob; Kleeman, Michelle; Szarewski, Anne

    2015-03-01

    Non-attenders for cervical screening are at increased risk of cervical cancer. Studies offering self-sampling for high-risk Human Papillomavirus (HrHPV) testing have shown greater uptake than sending another invitation for cytology. To explore whether uptake would increase in a less diverse, more stable population than the previous English study, which demonstrated a lower response rate than other studies. The primary objective was whether non-attenders were more likely to respond to a postal invitation, including kit, to collect a self-sample compared with a further invitation for cytology screening. The secondary objective was whether women with an abnormal result would attend for follow-up. 6000 non-attenders for screening in this pragmatic, randomized (1:1) controlled trial in Newcastle-upon-Tyne were sent an HPV self-sample kit (intervention) or a further invitation for cytology screening (comparator). 411(13%) responded to the intervention, returning a self-sample (247(8%)) or attending for cytology (164(5%)), compared with 183(6%) attending for cytology, relative risk 2.25 (95% CI 1.90-2.65) (comparator arm). Of those testing hrHPV positive (32(13%)), 19(59%) subsequently attended cytology screening. Of those in the intervention group who attended for cytology screening without returning an hrHPV self-sample (n = 164), 5% (n = 8) were referred for colposcopy - all attended. In the comparator group eight of the nine referred for colposcopy attended. Persistent non-responders to invitations for cervical screening are significantly more likely to respond to a postal invitation to return a self-collected sample for HPV testing than a further invitation for cytology screening. However, just over half followed up on this positive HPV result. © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

  4. Textbook images: how do they invite students into physics?

    NASA Astrophysics Data System (ADS)

    Bungum, Berit

    2013-09-01

    This paper presents a study of images in Norwegian physics textbooks for upper secondary school, and how they invite students into physics by means of visual communication. The concept of ‘framing’ is used to investigate how the perspective in the image provides a sense of participation. It is found that older textbooks, where objects and experimental setups dominate the images, involve the reader in terms of weak framing. This is to some degree resembled in newer textbooks with the increased use of photographs of learners in some physics activity. This does, however, invite the learner into school physics rather than professional physics. Connections made to physics in society and everyday life in images can also be seen as inviting the learner into physics, by exhibiting how modern physicists work and by referring to the relevance of physics, respectively.

  5. Analysis of AtGUS1 and AtGUS2 in Arabidopsis root apex by a highly sensitive TSA-MISH method.

    PubMed

    Bruno, Leonardo; Ronchini, Matteo; Gagliardi, Olimpia; Corinti, Tamara; Chiappetta, Adriana; Gerola, Paolo; Bitonti, Maria B

    2015-01-01

    A new highly sensitive whole-mount in situ hybridization method, based on tyramide signal amplification (TSA-MISH) was developed and a combined GFP detection and TSA-MISH procedure was applied for the first time in plants, to precisely define the spatial pattern of AtGUS1 and AtGUS2 expression in the root apex. β-glucuronidases (GUSs) belonging to the glycosyl hydrolases (GHs) 79 family, are widely distributed in plants, but their functional role has not yet been fully investigated. In the model system Arabidopsis Thaliana, three different AtGUS genes have been identified which encode proteins with putative different fates. Endogenous GUS expression has been detected in different organs and tissues, but the cyto-histological domains of gene expression remain unclear. The results here reported show co-expression of AtGUS1 and AtGUS2 in different functional zones of the root apex (the cap central zone, the root cap meristem, the staminal cell niche and the cortical cell layers of the proximal meristem), while AtGUS2 is exclusively expressed in the cap peripheral layer and in the epidermis in the elongation zone. Interestingly, both genes are not expressed in the stelar portion of the proximal meristem. A spatial (cortex vs. stele) and temporal (proximal meristem vs. transition zone) regulation of AtGUS1 and AtGUS2 expression is therefore active in the root apex. This expression pattern, although globally consistent with the involvement of GUS activity in both cell proliferation and elongation, clearly indicates that AtGUS1 and AtGUS2 could control distinct downstream process depending on the developmental context and the interaction with other players of root growth control. In the future, the newly developed approaches may well be very useful to dissect such interactions.

  6. 77 FR 28894 - Maritime Vulnerability Self-Assessment Tool

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-16

    ... DEPARTMENT OF HOMELAND SECURITY Transportation Security Administration Maritime Vulnerability Self... maritime vulnerability self- assessment tool. SUMMARY: The Transportation Security Administration (TSA... conducting vulnerability assessments became available and usage of the TMSARM has dropped off considerably...

  7. Unconscious bias when experts are invited - and the results of conscious action

    NASA Astrophysics Data System (ADS)

    Stadmark, Johanna; Conley, Daniel J.

    2016-04-01

    Visibility of different kinds is important in academic work. We publish papers, present results at conferences and are invited to give seminars and write contributions and reviews about other scientists' work. A few years back we reacted on the invitation-only sections in two of the leading scientific journals. We asked: "Who are invited to write about other researchers' newly published results?" The short answer could be: Experts in the field. However, we found that it was mainly male experts in the field when we reviewed/categorized the authors of the invitation-only sections News & Views and Perspective in Nature and Science, respectively, during the years 2010 and 2011 (in total 1663 authors). The proportion of female authors was compared to the proportion of female scientists within their respective fields within academia. We found that female scientists were invited less often than their male colleagues. We notified the two journals about the mismatch (Nature 2012: 488) and their encouraging reply was that they needed to improve how they reflect women's contributions to science (Nature 2012: 491). In 2013 and 2014 the invitation-only sections (with in total 1845 authors) had reached parity (compared to the pool of available scientists) for contributions regarding Earth and Environmental Science, and made steps forward for Medical/Biological/Chemical sciences and Physical sciences. By raising awareness about the unconscious biases we all have, e.g. both men and women, we can act to make progress towards parity in science.

  8. Inviting parents to take part in paediatric palliative care research: a mixed-methods examination of selection bias.

    PubMed

    Crocker, Joanna C; Beecham, Emma; Kelly, Paula; Dinsdale, Andrew P; Hemsley, June; Jones, Louise; Bluebond-Langner, Myra

    2015-03-01

    Recruitment to paediatric palliative care research is challenging, with high rates of non-invitation of eligible families by clinicians. The impact on sample characteristics is unknown. To investigate, using mixed methods, non-invitation of eligible families and ensuing selection bias in an interview study about parents' experiences of advance care planning (ACP). We examined differences between eligible families invited and not invited to participate by clinicians using (1) field notes of discussions with clinicians during the invitation phase and (2) anonymised information from the service's clinical database. Families were eligible for the ACP study if their child was receiving care from a UK-based tertiary palliative care service (Group A; N = 519) or had died 6-10 months previously having received care from the service (Group B; N = 73). Rates of non-invitation to the ACP study were high. A total of 28 (5.4%) Group A families and 21 (28.8%) Group B families (p < 0.0005) were invited. Family-clinician relationship appeared to be a key factor associated qualitatively with invitation in both groups. In Group A, out-of-hours contact with family was statistically associated with invitation (adjusted odds ratio 5.46 (95% confidence interval 2.13-14.00); p < 0.0005). Qualitative findings also indicated that clinicians' perceptions of families' wellbeing, circumstances, characteristics, engagement with clinicians and anticipated reaction to invitation influenced invitation. We found evidence of selective invitation practices that could bias research findings. Non-invitation and selection bias should be considered, assessed and reported in palliative care studies. © The Author(s) 2014.

  9. 43 CFR 10010.37 - Inviting comments.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... may be requested from local agencies through these procedures to the extent that they include the affected local jurisdictions. (b) When the proposed action may affect the environment of an Indian... POLICY ACT Environmental Impact Statements § 10010.37 Inviting comments. (a) Comments from State agencies...

  10. Inviting Success in Computer-Assisted Instruction.

    ERIC Educational Resources Information Center

    Henderson, Catherine

    This paper reviews briefly the essential characteristics of both invitational education and computer assisted instruction (CAI) and the ways in which coordination of these two models can produce stimulating and valuable educational experiences for students. A matrix illustrates the characteristics of CAI which can support the major values of…

  11. 43 CFR 46.435 - Inviting comments.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... ENVIRONMENTAL POLICY ACT OF 1969 Environmental Impact Statements § 46.435 Inviting comments. (a) A bureau must seek comment from the public as part of the Notice of Intent to prepare an environmental impact statement and notice of availability for a draft environmental impact statement; (b) In addition to...

  12. 43 CFR 46.435 - Inviting comments.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... ENVIRONMENTAL POLICY ACT OF 1969 Environmental Impact Statements § 46.435 Inviting comments. (a) A bureau must seek comment from the public as part of the Notice of Intent to prepare an environmental impact statement and notice of availability for a draft environmental impact statement; (b) In addition to...

  13. 48 CFR 14.203-2 - Dissemination of information concerning invitations for bids.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 14.203-2 Dissemination of information concerning invitations for bids. Procedures concerning display... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Dissemination of information concerning invitations for bids. 14.203-2 Section 14.203-2 Federal Acquisition Regulations System...

  14. 76 FR 35229 - Intent to Request Renewal and Amendment From OMB of One Current Public Collection of Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-16

    ... from TSA's Corporate Security Review (CSR) in that a CSR looks at corporate or company-wide security... DEPARTMENT OF HOMELAND SECURITY Transportation Security Administration Intent to Request Renewal... of the Top 100 Most Critical Pipelines AGENCY: Transportation Security Administration, DHS. ACTION...

  15. The Inviting Convicts to College Program

    ERIC Educational Resources Information Center

    Rose, Chris; Reschenberg, Kristin; Richards, Stephen

    2010-01-01

    While we know formal education is an important variable for reducing recidivism, there are few prison systems still offering college courses. We introduce the Inviting Convicts to College Program that deploys undergraduate student-teachers as instructors of college level courses inside prisons. The student-teachers are supervised by professors.…

  16. The histone deacetylase inhibitor, trichostatin A, inhibits the development of 2,4-dinitrofluorobenzene-induced dermatitis in NC/Nga mice.

    PubMed

    Kim, Tae-Ho; Jung, Jung-A; Kim, Gun-Dong; Jang, An-Hee; Cho, Jeong-Je; Park, Yong Seek; Park, Cheung-Seog

    2010-10-01

    Repetitive skin contact with a chemical hapten like 2,4-dinitrofluorobenzene (DNFB) evokes an atopic dermatitis (AD)-like dermatitis reaction in NC/Nga mice maintained under specific pathogen-free (SPF) conditions. The histone deacetylase (HDAC) inhibitor, trichostatin A (TSA), modulates the expression of several genes by inhibiting the activity of HDACs. Furthermore, TSA has been reported to suppress inflammatory cytokine expression and to induce T cell-suppression by increasing regulatory T cell (T reg cell) numbers. In addition, histone deacetylase inhibitors (HDACi) are currently undergoing clinical trials for the treatment of inflammatory disorders. In the present study, we examined whether treatment with TSA suppresses AD-like skin lesions in NC/Nga mice treated with DNFB under SPF conditions. Intraperitoneal (i.p.) administration of TSA to DNFB-treated NC/Nga mice was found to inhibit ear thickness increases and the skin lesions induced by DNFB. Furthermore, IL-4 production by CD4+ T cells from the lymph nodes of DNFB-treated NC/Nga mice was significantly inhibited by TSA, although levels of IFN-γ were not. Flow cytometric analysis of lymphocytes showed an increase in CD4+ CD25+ T cell proportions in mice given TSA-i.p. These findings suggest that TSA suppresses the development of AD-like dermatitis in DNFB-treated NC/Nga mice by reducing IL-4 production and increasing the T reg cell population. Copyright © 2010 Elsevier B.V. All rights reserved.

  17. Promoting Critical Ideas of Leadership, Culture and Diversity. The 2010 Yearbook of the National Council of Professors of Educational Administration

    ERIC Educational Resources Information Center

    Irby, Beverly J., Ed.; Alford, Betty J., Ed.; Perreault, George, Ed.; Zellner, Luana, Ed.

    2010-01-01

    This volume presents the 2010 Yearbook of the National Council of Professors of Educational Administration (NCPEA). This year's theme is "Promoting Critical Ideas of Leadership, Culture and Diversity." This yearbook contains five parts. Part 1, Invited Chapters, includes the following: (1) President's Message: Critical Issues in…

  18. Results of the Alternative Calendar Survey: A Survey of Faculty, Classified Staff and Administrators at California Community Colleges That Have Moved from an 18-Week Semester to a Compressed Calendar.

    ERIC Educational Resources Information Center

    Beachler, Judith

    From December 2002 through February 2003, Los Rios Community College District (LRCCD), California, administrators invited key faculty members, classified staff, and administrators of California community colleges that have moved from an 18 week semester to a compressed 15- or 16-week semester to share their colleges' experiences through the…

  19. A Socio-Pragmatic Comparative Study of Ostensible Invitations in English and Farsi

    ERIC Educational Resources Information Center

    Salmani-Nodoushan, Mohammad Ali

    2006-01-01

    In their study in 1990, Clark and Isaacs identified five properties and seven defining features that distinguished between English ostensible and genuine invitations. To see if Persian ostensible and genuine invitations could be distinguished by the same features and properties, the present study was carried out. 45 field workers observed and…

  20. 78 FR 30319 - Intent to Request Renewal From OMB of One Current Public Collection of Information: Security...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-22

    ... DEPARTMENT OF HOMELAND SECURITY Transportation Security Administration [Docket No. TSA-2002-11602] Intent to Request Renewal From OMB of One Current Public Collection of Information: Security Programs for..., Transportation Security Administration, 601 South 12th Street, Arlington, VA 20598-6011. FOR FURTHER INFORMATION...

  1. 77 FR 24506 - Extension of Agency Information Collection Activity Under OMB Review: Air Cargo Security...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-24

    ...This notice announces that the Transportation Security Administration (TSA) has forwarded the Information Collection Request (ICR), Office of Management and Budget (OMB) control number 1652-0040, abstracted below to OMB for review and approval of an extension of the currently approved collection under the Paperwork Reduction Act (PRA). The ICR describes the nature of the information collection and its expected burden. TSA published a Federal Register notice, with a 60-day comment period soliciting comments, of the following collection of information on February 24, 2012, 77 FR 11145. TSA has not received any comments. The collection of information that make up this ICR involve five broad categories affecting airports, passenger aircraft operators, foreign air carriers, indirect air carriers and all-cargo carriers operating under a TSA-approved security program. These five categories are: Security programs, security threat assessments (STAs), known shipper data via the Known Shipper Management System (KSMS), cargo screening reporting, and evidence of compliance recordkeeping.

  2. Potentials Unlimited: Nursing Practice, Education and Administration. Nursing Research Conference Proceedings (1st, Fresno, California, April 8, 1987).

    ERIC Educational Resources Information Center

    Jacobsen-Webb, Marilyn-Lu, Ed.

    The purposes of this conference were: (1) to present research representing a variety of topics and study designs; (2) to disseminate findings of nursing research; and (3) to stimulate the use of research and theory as a basis for clinical, educational, and administrative practice. The keynote address and two invited presentations are presented in…

  3. 19 CFR 122.75b - Electronic manifest requirement for crew members and non-crew members onboard commercial aircraft...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Aviation Administration (FAA) Aviation Safety Inspectors with valid credentials and authorization are not... TSA for compliance with an aviation security program, emergency amendment, or security directive...

  4. Evaluating the effectiveness of biometric access control systems

    NASA Astrophysics Data System (ADS)

    Lively, Valerie M.

    2005-05-01

    This paper describes the contribution by the National Safe Skies Alliance (Safe Skies) in operational testing of biometric access control systems under the guidance of the Transportation Security Administration (TSA). Safe Skies has been conducting operational tests of biometric access control systems on behalf of the TSA for approximately four years. The majority of this testing has occurred at the McGhee Tyson Airport (TYS) in Knoxville, Tennessee. Twelve separate biometric devices - eight fingerprint, facial, iris, hand geometry, and fingerprint and iris, have been tested to date. Tests were conducted at a TYS administrative door and different airports to evaluate the access control device under normal, abnormal, and attempt-to-defeat conditions.

  5. Method of invitation and geographical proximity as predictors of NHS Health Check uptake.

    PubMed

    Gidlow, Christopher; Ellis, Naomi; Randall, Jason; Cowap, Lisa; Smith, Graham; Iqbal, Zafar; Kumar, Jagdish

    2015-06-01

    Uptake of NHS Health Checks remains below the national target. Better understanding of predictors of uptake can inform targeting and delivery. We explored invitation method and geographical proximity as predictors of uptake in deprived urban communities. This observational cohort study used data from all 4855 individuals invited for an NHS Health Check (September 2010-February 2014) at five general practices in Stoke-on-Trent, UK. Attendance/non-attendance was the binary outcome variable. Predictor variables included the method of invitation, general practice, demographics, deprivation and distance to Health Check location. Mean attendance (61.6%) was above the city and national average, but varied by practice (47.5-83.3%; P < 0.001). Telephone/verbal invitations were associated with higher uptake than postal invitations (OR = 2.87, 95% CI = 2.26-3.64), yet significant practice-level variation remained. Distance to Health Check was not associated with attendance. Increasing age (OR = 1.04, 95% CI = 1.03-1.04), female gender (OR = 1.48, 95% CI = 1.30-1.68) and living in the least deprived areas (OR = 1.59, 95% CI = 1.23-2.05) were all independent positive predictors of attendance. Using verbal or telephone invitations should be considered to improve Health Check uptake. Other differences in recruitment and delivery that might explain remaining practice-level variation in uptake warrant further exploration. Geographical proximity may not be an important predictor of uptake in urban populations. © The Author 2014. Published by Oxford University Press on behalf of Faculty of Public Health.

  6. 75 FR 7978 - Privacy Act of 1974: Implementation of Exemptions; Department of Homeland Security Transportation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-23

    ...; Department of Homeland Security Transportation Security Administration-023 Workplace Violence Prevention... Administration-023 Workplace Violence Prevention Program System of Records and this proposed rulemaking. In this... Privacy Act (5 U.S.C. 552a) titled, DHS/TSA-023 Workplace Violence Prevention Program System of Records...

  7. 49 CFR 1503.403 - Delegation of authority.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false Delegation of authority. 1503.403 Section 1503.403 Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION... Assessment of Civil Penalties by TSA § 1503.403 Delegation of authority. The Administrator delegates the...

  8. Gender parity trends for invited speakers at four prominent virology conference series.

    PubMed

    Kalejta, Robert F; Palmenberg, Ann C

    2017-06-07

    Scientific conferences are most beneficial to participants when they showcase significant new experimental developments, accurately summarize the current state of the field, and provide strong opportunities for collaborative networking. A top-notch slate of invited speakers, assembled by conference organizers or committees, is key to achieving these goals. The perceived underrepresentation of female speakers at prominent scientific meetings is currently a popular topic for discussion, but one that often lacks supportive data. We compiled the full rosters of invited speakers over the last 35 years for four prominent international virology conferences, the American Society for Virology Annual Meeting (ASV), the International Herpesvirus Workshop (IHW), the Positive-Strand RNA Virus Symposium (PSR), and the Gordon Research Conference on Viruses & Cells (GRC). The rosters were cross-indexed by unique names, gender, year, and repeat invitations. When plotted as gender-dependent trends over time, all four conferences showed a clear proclivity for male-dominated invited speaker lists. Encouragingly, shifts toward parity are emerging within all units, but at different rates. Not surprisingly, both selection of a larger percentage of first time participants and the presence of a woman on the speaker selection committee correlated with improved parity. Session chair information was also collected for the IHW and GRC. These visible positions also displayed a strong male dominance over time that is eroding slowly. We offer our personal interpretation of these data to aid future organizers achieve improved equity among the limited number of available positions for session moderators and invited speakers. IMPORTANCE Politicians and media members have a tendency to cite anecdotes as conclusions without any supporting data. This happens so frequently now, that a name for it has emerged: fake news. Good science proceeds otherwise. The under representation of women as invited

  9. Gender Parity Trends for Invited Speakers at Four Prominent Virology Conference Series

    PubMed Central

    Palmenberg, Ann C.

    2017-01-01

    ABSTRACT Scientific conferences are most beneficial to participants when they showcase significant new experimental developments, accurately summarize the current state of the field, and provide strong opportunities for collaborative networking. A top-notch slate of invited speakers, assembled by conference organizers or committees, is key to achieving these goals. The perceived underrepresentation of female speakers at prominent scientific meetings is currently a popular topic for discussion, but one that often lacks supportive data. We compiled the full rosters of invited speakers over the last 35 years for four prominent international virology conferences, the American Society for Virology Annual Meeting (ASV), the International Herpesvirus Workshop (IHW), the Positive-Strand RNA Virus Symposium (PSR), and the Gordon Research Conference on Viruses & Cells (GRC). The rosters were cross-indexed by unique names, gender, year, and repeat invitations. When plotted as gender-dependent trends over time, all four conferences showed a clear proclivity for male-dominated invited speaker lists. Encouragingly, shifts toward parity are emerging within all units, but at different rates. Not surprisingly, both selection of a larger percentage of first-time participants and the presence of a woman on the speaker selection committee correlated with improved parity. Session chair information was also collected for the IHW and GRC. These visible positions also displayed a strong male dominance over time that is eroding slowly. We offer our personal interpretation of these data to aid future organizers achieve improved equity among the limited number of available positions for session moderators and invited speakers. IMPORTANCE Politicians and media members have a tendency to cite anecdotes as conclusions without any supporting data. This happens so frequently now, that a name for it has emerged: fake news. Good science proceeds otherwise. The underrepresentation of women as

  10. Response Rates for Patient-Reported Outcomes Using Web-Based Versus Paper Questionnaires: Comparison of Two Invitational Methods in Older Colorectal Cancer Patients

    PubMed Central

    Vissers, Pauline AJ; Mols, Floortje; Thong, Melissa SY; van de Poll-Franse, Lonneke V

    2015-01-01

    Background Improving questionnaire response rates is an everlasting issue for research. Today, the Internet can easily be used to collect data quickly. However, collecting data on the Internet can lead to biased samples because not everyone is able to access or use the Internet. The older population, for example, is much less likely to use the Internet. The Patient-Reported Outcomes Following Initial Treatment and Long-Term Evaluation of Survivorship (PROFILES) registry offers a platform to collect Web-based and paper questionnaires and to try different measures to improve response rates. Objective In this study, our aim was to study the influence of two methods of invitation on the response rate. Our second aim was to examine the preference of questionnaire mode of administration (paper or Web-based) for the older patient in particular. Methods To test these two invitational methods, 3406 colorectal cancer patients between ages 18 and 85 years received an invitation containing an access code for the Web-based questionnaire. They could also request a paper questionnaire with an included reply card (paper-optional group). In contrast, 179 randomly selected colorectal cancer patients received a paper questionnaire with the invitation (paper-included group). They could also choose to fill out the Web-based questionnaire with the included access code. Results Response rates did not differ between the paper-optional and the paper-included groups (73.14%, 2491/3406 and 74.9%, 134/179, P=.57). In the paper-optional group, online response was significantly higher when compared to the paper-included group (41.23%, 1027/2491 vs 12.7%, 17/134, P<.001). The majority of online respondents responded after the first invitation (95.33%, 979/1027), which was significantly higher than the paper respondents (52.19%, 764/1464, P<.001). Respondents aged 70 years and older chose to fill out a paper questionnaire more often (71.0%, 677/954). In the oldest age group (≥80 years), 18

  11. 75 FR 80838 - Notice of Invitation to Participate In Coal Exploration License, Utah

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-23

    ...] Notice of Invitation to Participate In Coal Exploration License, Utah AGENCY: Bureau of Land Management, Interior. ACTION: Notice of Invitation to Participate in Coal Exploration License. SUMMARY: All interested... program for the exploration of coal deposits owned by the United States of America in Emery County, Utah...

  12. Using Invitational Learning to Address Writing Competence for Middle School Students with Disabilities

    ERIC Educational Resources Information Center

    Ornelles, Cecily; Black, Rhonda S.

    2012-01-01

    This study describes the process of creating an Invitational Learning environment to improve the writing competence of middle school students in two special education classes. Teacher-student interactions were coded according to Purkey and Novak's (1996) Intentionality/Invitation Quadrant with levels corresponding to intentionally disinviting,…

  13. 78 FR 24353 - Provisions for Fees Related to Hazardous Materials Endorsements and Transportation Worker...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-25

    ... Mitchell, Office of Intelligence and Analysis (OIA), TSA-10, Transportation Security Administration, 601... Management System (FDMS) Web page at http://www.regulations.gov ; (2) Accessing the Government Printing...

  14. 41 CFR Appendix B to Part 60 - 741-Sample Invitation To Self-Identify

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Relating to Public Contracts OFFICE OF FEDERAL CONTRACT COMPLIANCE PROGRAMS, EQUAL EMPLOYMENT OPPORTUNITY... Invitation To Self-Identify Note: When the invitation to self-identify is being extended prior to an offer of... employer is a Government contractor subject to section 503 of the Rehabilitation Act of 1973, as amended...

  15. 41 CFR Appendix B to Part 60 - 741-Sample Invitation To Self-Identify

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Relating to Public Contracts OFFICE OF FEDERAL CONTRACT COMPLIANCE PROGRAMS, EQUAL EMPLOYMENT OPPORTUNITY... Invitation To Self-Identify Note: When the invitation to self-identify is being extended prior to an offer of... employer is a Government contractor subject to section 503 of the Rehabilitation Act of 1973, as amended...

  16. 41 CFR Appendix B to Part 60 - 741-Sample Invitation To Self-Identify

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Relating to Public Contracts OFFICE OF FEDERAL CONTRACT COMPLIANCE PROGRAMS, EQUAL EMPLOYMENT OPPORTUNITY... Invitation To Self-Identify Note: When the invitation to self-identify is being extended prior to an offer of... employer is a Government contractor subject to section 503 of the Rehabilitation Act of 1973, as amended...

  17. 76 FR 51847 - Air Cargo Screening

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-18

    ...This rule amends two provisions of the Air Cargo Screening Interim Final Rule (IFR) issued on September 16, 2009, and responds to public comments on the IFR. The IFR codified a statutory requirement of the Implementing Recommendations of the 9/11 Commission Act of 2007 that the Transportation Security Administration (TSA) establish a system to screen 100 percent of cargo transported on passenger aircraft not later than August 3, 2010. It established the Certified Cargo Screening Program, in which TSA certifies shippers, indirect air carriers, and other entities as Certified Cargo Screening Facilities (CCSFs) to screen cargo prior to transport on passenger aircraft. Under the IFR, each CCSF applicant had to successfully undergo an assessment of their facility by a TSA-approved validation firm or by TSA. In response to public comment, this Final Rule removes all validation firm and validator provisions, so that TSA will continue to conduct assessments of the applicant's facility to determine if certification is appropriate. The IFR also required that if an aircraft operator or foreign air carrier screens cargo off an airport, it must do so as a CCSF. The Final Rule deletes this requirement, as aircraft operators are already screening cargo on airport under a TSA-approved security program, and do not need a separate certification to screen cargo off airport. This rule also proposes a fee range for the processing of Security Threat Assessments, and seeks comment on the proposed fee range and the methodology used to develop the fee. TSA will announce the final fee in a future Federal Register notice.

  18. Remember Our Mission: Making Education and Schools Better for Students. The 2009 Yearbook of the National Council of Professors of Educational Administration

    ERIC Educational Resources Information Center

    Achilles, Charles M., Ed.; Irby, Beverly J., Ed.; Alford, Betty, Ed.; Perreault, George, Ed.

    2009-01-01

    This volume presents the 2009 Yearbook of the National Council of Professors of Educational Administration. This year's theme is "Remembering Our Mission: Making Education and Schools Better for Students." This Yearbook contains six parts. Part 1, Invited Chapters, includes the following: (1) President's Message: Remembering Our Mission…

  19. From School Militarization to School Securitization: National Security Finds Its Place in Schools

    ERIC Educational Resources Information Center

    Nguyen, Nicole

    2017-01-01

    This ethnography explores daily life at Milton High School, a US public school with its own specialized Homeland Security program. From "military grunts" serving in distant theaters of war to Transportation Security Administration (TSA) agents defending the US borderlands to National Security Administration (NSA) technicians monitoring…

  20. 78 FR 37234 - Notice of Invitation; Coal Exploration License Application NDM 105349, ND

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-20

    ...] Notice of Invitation; Coal Exploration License Application NDM 105349, ND AGENCY: Bureau of Land Management, Interior. ACTION: Notice. SUMMARY: Members of the public are invited to participate with BNI Coal Ltd. on a pro rata cost sharing basis in a program for the exploration of coal deposits owned by the...

  1. 78 FR 25531 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel NIRVANA; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-01

    ...As authorized by 46 U.S.C. 12121, the Secretary of Transportation, as represented by the Maritime Administration (MARAD), is authorized to grant waivers of the U.S.-build requirement of the coastwise laws under certain circumstances. A request for such a waiver has been received by MARAD. The vessel, and a brief description of the proposed service, is listed below.

  2. 41 CFR Appendix B to Part 60 - 300-Sample Invitation to Self-identify

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Self-identify B Appendix B to Part 60 Public Contracts and Property Management Other Provisions... FORCES SERVICE MEDAL VETERANS Pt. 60-300, App. B Appendix B to Part 60-300—Sample Invitation to Self-identify Note: When the invitation to self-identify is being extended to disabled veterans prior to an...

  3. 41 CFR Appendix B to Part 60 - 300-Sample Invitation to Self-identify

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Self-identify B Appendix B to Part 60 Public Contracts and Property Management Other Provisions... FORCES SERVICE MEDAL VETERANS Pt. 60-300, App. B Appendix B to Part 60-300—Sample Invitation to Self-identify Note: When the invitation to self-identify is being extended to disabled veterans prior to an...

  4. 41 CFR Appendix B to Part 60 - 250-Sample Invitation To Self-Identify

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true 250-Sample Invitation To Self-Identify B Appendix B to Part 60 Public Contracts and Property Management Other Provisions... OTHER PROTECTED VETERANS Pt. 60-250, App. B Appendix B to Part 60-250—Sample Invitation To Self-Identify...

  5. 41 CFR Appendix B to Part 60 - 250-Sample Invitation To Self-Identify

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 41 Public Contracts and Property Management 1 2013-07-01 2013-07-01 false 250-Sample Invitation To Self-Identify B Appendix B to Part 60 Public Contracts and Property Management Other Provisions... OTHER PROTECTED VETERANS Pt. 60-250, App. B Appendix B to Part 60-250—Sample Invitation To Self-Identify...

  6. 41 CFR Appendix B to Part 60 - 250-Sample Invitation To Self-Identify

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 41 Public Contracts and Property Management 1 2012-07-01 2009-07-01 true 250-Sample Invitation To Self-Identify B Appendix B to Part 60 Public Contracts and Property Management Other Provisions... OTHER PROTECTED VETERANS Pt. 60-250, App. B Appendix B to Part 60-250—Sample Invitation To Self-Identify...

  7. 41 CFR Appendix B to Part 60 - 250-Sample Invitation To Self-Identify

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 41 Public Contracts and Property Management 1 2011-07-01 2009-07-01 true 250-Sample Invitation To Self-Identify B Appendix B to Part 60 Public Contracts and Property Management Other Provisions... OTHER PROTECTED VETERANS Pt. 60-250, App. B Appendix B to Part 60-250—Sample Invitation To Self-Identify...

  8. 49 CFR 1549.3 - TSA inspection authority.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...) Oversee the implementation, and ensure the adequacy, of security measures at airports and other... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY CERTIFIED CARGO SCREENING PROGRAM General § 1549.3...

  9. 49 CFR 1549.3 - TSA inspection authority.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...) Oversee the implementation, and ensure the adequacy, of security measures at airports and other... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY CERTIFIED CARGO SCREENING PROGRAM General § 1549.3...

  10. 49 CFR 1549.3 - TSA inspection authority.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...) Oversee the implementation, and ensure the adequacy, of security measures at airports and other... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY CERTIFIED CARGO SCREENING PROGRAM General § 1549.3...

  11. 49 CFR 1549.3 - TSA inspection authority.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...) Oversee the implementation, and ensure the adequacy, of security measures at airports and other... Transportation Other Regulations Relating to Transportation (Continued) TRANSPORTATION SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY CERTIFIED CARGO SCREENING PROGRAM General § 1549.3...

  12. Enabling narrative pedagogy: inviting, waiting, and letting be.

    PubMed

    Ironside, Pamela M

    2014-01-01

    This article describes how teachers enable Narrative Pedagogy in their courses by explicating the Concernful Practice Inviting: Waiting and Letting Be. Narrative Pedagogy, a research-based, phenomenological approach to teaching and learning, extends conventional pedagogies and offers nursing faculty an alternative way of transforming their schools and courses. Using hermeneutic phenomenology, interview data collected over a 10-year period were analyzed by coding practical examples of teachers' efforts to enact Narrative Pedagogy. When Narrative Pedagogy is enacted, teachers and students focus on thinking and learning together about nursing phenomena and seek new understandings about how they may provide care in the myriad situations they encounter. Although the Concernful Practices co-occur, explicating inviting experiences can assist new teachers, and those seeking to extend their pedagogical literacy, by providing new understandings of how Narrative Pedagogy can be enacted.

  13. Preparing for the Next Kamikaze Attack on the American Homeland

    DTIC Science & Technology

    2007-11-27

    This paper presents a case for not diverting additional Transportation Security Administration (TSA) resources from Civil Aviation security to...divert additional resources from Civil Aviation security to General Aviation security .

  14. Homeland Security Department: FY2008 Appropriations

    DTIC Science & Technology

    2007-07-17

    Security Administration (TSA); $8,457 million for the U.S. Coast Guard; $1,399 million for the Secret Service; $1,047 for the National Protection and...U.S. Coast Guard; $1,396 million for the Secret Service; $1,035 for the NPP; $7,239 million for FEMA; $30 million for USCIS; $777 million for S&T...for CBP; $4,433 million for ICE; $3,685 million for the TSA; $8,559 million for the U.S. Coast Guard; $1,396 million for the Secret Service; $919 for

  15. 49 CFR 1540.117 - Threat assessments regarding aliens holding or applying for FAA certificates, ratings, or...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... security threat. (b) Definitions. The following terms apply in this section: Assistant Administrator means the Assistant Administrator for Intelligence for TSA. Date of service means— (1) The date of personal delivery in the case of personal service; (2) The mailing date shown on the certificate of service; (3) The...

  16. 49 CFR 1540.117 - Threat assessments regarding aliens holding or applying for FAA certificates, ratings, or...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... security threat. (b) Definitions. The following terms apply in this section: Assistant Administrator means the Assistant Administrator for Intelligence for TSA. Date of service means— (1) The date of personal delivery in the case of personal service; (2) The mailing date shown on the certificate of service; (3) The...

  17. 49 CFR 1540.117 - Threat assessments regarding aliens holding or applying for FAA certificates, ratings, or...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... security threat. (b) Definitions. The following terms apply in this section: Assistant Administrator means the Assistant Administrator for Intelligence for TSA. Date of service means— (1) The date of personal delivery in the case of personal service; (2) The mailing date shown on the certificate of service; (3) The...

  18. 49 CFR 1540.117 - Threat assessments regarding aliens holding or applying for FAA certificates, ratings, or...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... security threat. (b) Definitions. The following terms apply in this section: Assistant Administrator means the Assistant Administrator for Intelligence for TSA. Date of service means— (1) The date of personal delivery in the case of personal service; (2) The mailing date shown on the certificate of service; (3) The...

  19. 49 CFR 1540.117 - Threat assessments regarding aliens holding or applying for FAA certificates, ratings, or...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... security threat. (b) Definitions. The following terms apply in this section: Assistant Administrator means the Assistant Administrator for Intelligence for TSA. Date of service means— (1) The date of personal delivery in the case of personal service; (2) The mailing date shown on the certificate of service; (3) The...

  20. 78 FR 73868 - Privacy Act of 1974; Department of Homeland Security Transportation Security Administration-DHS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-09

    ... transportation systems to ensure freedom of movement for people and commerce. To achieve this mission, TSA is... security screening and identity verification of individuals, including identification media and identifying... addresses, phone numbers); Social Security Number, Fingerprints or other biometric identifiers; Photographs...

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