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Sample records for advisor model sam

  1. System Advisor Model, SAM 2014.1.14: General Description

    SciTech Connect

    Blair, N.; Dobos, A. P.; Freeman, J.; Neises, T.; Wagner, M.; Ferguson, T.; Gilman, P.; Janzou, S.

    2014-02-01

    This document describes the capabilities of the U.S. Department of Energy and National Renewable Energy Laboratory's System Advisor Model (SAM), Version 2013.9.20, released on September 9, 2013. SAM is a computer model that calculates performance and financial metrics of renewable energy systems. Project developers, policy makers, equipment manufacturers, and researchers use graphs and tables of SAM results in the process of evaluating financial, technology, and incentive options for renewable energy projects. SAM simulates the performance of photovoltaic, concentrating solar power, solar water heating, wind, geothermal, biomass, and conventional power systems. The financial model can represent financial structures for projects that either buy and sell electricity at retail rates (residential and commercial) or sell electricity at a price determined in a power purchase agreement (utility). SAM's advanced simulation options facilitate parametric and sensitivity analyses, and statistical analysis capabilities are available for Monte Carlo simulation and weather variability (P50/P90) studies. SAM can also read input variables from Microsoft Excel worksheets. For software developers, the SAM software development kit (SDK) makes it possible to use SAM simulation modules in their applications written in C/C++, C#, Java, Python, and MATLAB. NREL provides both SAM and the SDK as free downloads at http://sam.nrel.gov. Technical support and more information about the software are available on the website.

  2. Wind Technology Modeling Within the System Advisor Model (SAM) (Poster)

    SciTech Connect

    Blair, N.; Dobos, A.; Ferguson, T.; Freeman, J.; Gilman, P.; Whitmore, J.

    2014-05-01

    This poster provides detail for implementation and the underlying methodology for modeling wind power generation performance in the National Renewable Energy Laboratory's (NREL's) System Advisor Model (SAM). SAM's wind power model allows users to assess projects involving one or more large or small wind turbines with any of the detailed options for residential, commercial, or utility financing. The model requires information about the wind resource, wind turbine specifications, wind farm layout (if applicable), and costs, and provides analysis to compare the absolute or relative impact of these inputs. SAM is a system performance and economic model designed to facilitate analysis and decision-making for project developers, financers, policymakers, and energy researchers. The user pairs a generation technology with a financing option (residential, commercial, or utility) to calculate the cost of energy over the multi-year project period. Specifically, SAM calculates the value of projects which buy and sell power at retail rates for residential and commercial systems, and also for larger-scale projects which operate through a power purchase agreement (PPA) with a utility. The financial model captures complex financing and rate structures, taxes, and incentives.

  3. System Advisor Model, SAM 2011.12.2: General Description

    SciTech Connect

    Gilman, P.; Dobos, A.

    2012-02-01

    This document describes the capabilities of the U.S. Department of Energy and National Renewable Energy Laboratory's System Advisor Model (SAM), Version 2011.12.2, released on December 2, 2011. SAM is software that models the cost and performance of renewable energy systems. Project developers, policy makers, equipment manufacturers, and researchers use graphs and tables of SAM results in the process of evaluating financial, technology, and incentive options for renewable energy projects. SAM simulates the performance of solar, wind, geothermal, biomass, and conventional power systems. The financial model can represent financing structures for projects that either buy and sell electricity at retail rates (residential and commercial) or sell electricity at a price determined in a power purchase agreement (utility). Advanced analysis options facilitate parametric, sensitivity, and statistical analyses, and allow for interfacing SAM with Microsoft Excel or with other computer programs. SAM is available as a free download at http://sam.nrel.gov. Technical support and more information about the software are available on the website.

  4. Case Studies Comparing System Advisor Model (SAM) Results to Real Performance Data: Preprint

    SciTech Connect

    Blair, N.; Dobos, A.; Sather, N.

    2012-06-01

    NREL has completed a series of detailed case studies comparing the simulations of the System Advisor Model (SAM) and measured performance data or published performance expectations. These case studies compare PV measured performance data with simulated performance data using appropriate weather data. The measured data sets were primarily taken from NREL onsite PV systems and weather monitoring stations.

  5. Parabolic Trough Reference Plant for Cost Modeling with the Solar Advisor Model (SAM)

    SciTech Connect

    Turchi, C.

    2010-07-01

    This report describes a component-based cost model developed for parabolic trough solar power plants. The cost model was developed by the National Renewable Energy Laboratory (NREL), assisted by WorleyParsons Group Inc., for use with NREL's Solar Advisor Model (SAM). This report includes an overview and explanation of the model, two summary contract reports from WorleyParsons, and an Excel spreadsheet for use with SAM. The cost study uses a reference plant with a 100-MWe capacity and six hours of thermal energy storage. Wet-cooling and dry-cooling configurations are considered. The spreadsheet includes capital and operating cost by component to allow users to estimate the impact of changes in component costs.

  6. Molten Salt Power Tower Cost Model for the System Advisor Model (SAM)

    SciTech Connect

    Turchi, C. S.; Heath, G. A.

    2013-02-01

    This report describes a component-based cost model developed for molten-salt power tower solar power plants. The cost model was developed by the National Renewable Energy Laboratory (NREL), using data from several prior studies, including a contracted analysis from WorleyParsons Group, which is included herein as an Appendix. The WorleyParsons' analysis also estimated material composition and mass for the plant to facilitate a life cycle analysis of the molten salt power tower technology. Details of the life cycle assessment have been published elsewhere. The cost model provides a reference plant that interfaces with NREL's System Advisor Model or SAM. The reference plant assumes a nominal 100-MWe (net) power tower running with a nitrate salt heat transfer fluid (HTF). Thermal energy storage is provided by direct storage of the HTF in a two-tank system. The design assumes dry-cooling. The model includes a spreadsheet that interfaces with SAM via the Excel Exchange option in SAM. The spreadsheet allows users to estimate the costs of different-size plants and to take into account changes in commodity prices. This report and the accompanying Excel spreadsheet can be downloaded at https://sam.nrel.gov/cost.

  7. Parabolic Trough Collector Cost Update for the System Advisor Model (SAM)

    SciTech Connect

    Kurup, Parthiv; Turchi, Craig S.

    2015-11-01

    This report updates the baseline cost for parabolic trough solar fields in the United States within NREL's System Advisor Model (SAM). SAM, available at no cost at https://sam.nrel.gov/, is a performance and financial model designed to facilitate decision making for people involved in the renewable energy industry. SAM is the primary tool used by NREL and the U.S. Department of Energy (DOE) for estimating the performance and cost of concentrating solar power (CSP) technologies and projects. The study performed a bottom-up build and cost estimate for two state-of-the-art parabolic trough designs -- the SkyTrough and the Ultimate Trough. The SkyTrough analysis estimated the potential installed cost for a solar field of 1500 SCAs as $170/m2 +/- $6/m2. The investigation found that SkyTrough installed costs were sensitive to factors such as raw aluminum alloy cost and production volume. For example, in the case of the SkyTrough, the installed cost would rise to nearly $210/m2 if the aluminum alloy cost was $1.70/lb instead of $1.03/lb. Accordingly, one must be aware of fluctuations in the relevant commodities markets to track system cost over time. The estimated installed cost for the Ultimate Trough was only slightly higher at $178/m2, which includes an assembly facility of $11.6 million amortized over the required production volume. Considering the size and overall cost of a 700 SCA Ultimate Trough solar field, two parallel production lines in a fully covered assembly facility, each with the specific torque box, module and mirror jigs, would be justified for a full CSP plant.

  8. System Advisor Model

    2010-03-01

    The System Advisor Model (SAM) is a performance and economic model designed to facilitate decision making for people involved in the renewable energy industry, ranging from project managers and engineers to incentive program designers, technology developers, and researchers.

  9. SAM Photovoltaic Model Technical Reference

    SciTech Connect

    Gilman, P.

    2015-05-27

    This manual describes the photovoltaic performance model in the System Advisor Model (SAM). The U.S. Department of Energy’s National Renewable Energy Laboratory maintains and distributes SAM, which is available as a free download from https://sam.nrel.gov. These descriptions are based on SAM 2015.1.30 (SSC 41).

  10. NREL's System Advisor Model Simplifies Complex Energy Analysis (Fact Sheet)

    SciTech Connect

    Not Available

    2015-01-01

    NREL has developed a tool -- the System Advisor Model (SAM) -- that can help decision makers analyze cost, performance, and financing of any size grid-connected solar, wind, or geothermal power project. Manufacturers, engineering and consulting firms, research and development firms, utilities, developers, venture capital firms, and international organizations use SAM for end-to-end analysis that helps determine whether and how to make investments in renewable energy projects.

  11. System Advisor Model: Flat Plate Photovoltaic Performance Modeling Validation Report

    SciTech Connect

    Freeman, J.; Whitmore, J.; Kaffine, L.; Blair, N.; Dobos, A. P.

    2013-12-01

    The System Advisor Model (SAM) is a free software tool that performs detailed analysis of both system performance and system financing for a variety of renewable energy technologies. This report provides detailed validation of the SAM flat plate photovoltaic performance model by comparing SAM-modeled PV system generation data to actual measured production data for nine PV systems ranging from 75 kW to greater than 25 MW in size. The results show strong agreement between SAM predictions and field data, with annualized prediction error below 3% for all fixed tilt cases and below 8% for all one axis tracked cases. The analysis concludes that snow cover and system outages are the primary sources of disagreement, and other deviations resulting from seasonal biases in the irradiation models and one axis tracking issues are discussed in detail.

  12. Generic CSP Performance Model for NREL's System Advisor Model: Preprint

    SciTech Connect

    Wagner, M. J.; Zhu, G.

    2011-08-01

    The suite of concentrating solar power (CSP) modeling tools in NREL's System Advisor Model (SAM) includes technology performance models for parabolic troughs, power towers, and dish-Stirling systems. Each model provides the user with unique capabilities that are catered to typical design considerations seen in each technology. Since the scope of the various models is generally limited to common plant configurations, new CSP technologies, component geometries, and subsystem combinations can be difficult to model directly in the existing SAM technology models. To overcome the limitations imposed by representative CSP technology models, NREL has developed a 'Generic Solar System' (GSS) performance model for use in SAM. This paper discusses the formulation and performance considerations included in this model and verifies the model by comparing its results with more detailed models.

  13. Comparison of Photovoltaic Models in the System Advisor Model: Preprint

    SciTech Connect

    Blair, N. J.; Dobos, A. P.; Gilman, P.

    2013-08-01

    The System Advisor Model (SAM) is free software developed by the National Renewable Energy Laboratory (NREL) for predicting the performance of renewable energy systems and analyzing the financial feasibility of residential, commercial, and utility-scale grid-connected projects. SAM offers several options for predicting the performance of photovoltaic (PV) systems. The model requires that the analyst choose from three PV system models, and depending on that choice, possibly choose from three module and two inverter component models. To obtain meaningful results from SAM, the analyst must be aware of the differences between the model options and their applicability to different modeling scenarios. This paper presents an overview the different PV model options and presents a comparison of results for a 200-kW system using different model options.

  14. Solar Advisor Model User Guide for Version 2.0

    SciTech Connect

    Gilman, P.; Blair, N.; Mehos, M.; Christensen, C.; Janzou, S.; Cameron, C.

    2008-08-01

    The Solar Advisor Model (SAM) provides a consistent framework for analyzing and comparing power system costs and performance across the range of solar technologies and markets, from photovoltaic systems for residential and commercial markets to concentrating solar power and large photovoltaic systems for utility markets. This manual describes Version 2.0 of the software, which can model photovoltaic and concentrating solar power technologies for electric applications for several markets. The current version of the Solar Advisor Model does not model solar heating and lighting technologies.

  15. Technical Manual for the SAM Physical Trough Model

    SciTech Connect

    Wagner, M. J.; Gilman, P.

    2011-06-01

    NREL, in conjunction with Sandia National Lab and the U.S Department of Energy, developed the System Advisor Model (SAM) analysis tool for renewable energy system performance and economic analysis. This paper documents the technical background and engineering formulation for one of SAM's two parabolic trough system models in SAM. The Physical Trough model calculates performance relationships based on physical first principles where possible, allowing the modeler to predict electricity production for a wider range of component geometries than is possible in the Empirical Trough model. This document describes the major parabolic trough plant subsystems in detail including the solar field, power block, thermal storage, piping, auxiliary heating, and control systems. This model makes use of both existing subsystem performance modeling approaches, and new approaches developed specifically for SAM.

  16. Technoeconomic Modeling of Battery Energy Storage in SAM

    SciTech Connect

    DiOrio, Nicholas; Dobos, Aron; Janzou, Steven; Nelson, Austin; Lundstrom, Blake

    2015-09-01

    Detailed comprehensive lead-acid and lithium-ion battery models have been integrated with photovoltaic models in an effort to allow System Advisor Model (SAM) to offer the ability to predict the performance and economic benefit of behind the meter storage. In a system with storage, excess PV energy can be saved until later in the day when PV production has fallen, or until times of peak demand when it is more valuable. Complex dispatch strategies can be developed to leverage storage to reduce energy consumption or power demand based on the utility rate structure. This document describes the details of the battery performance and economic models in SAM.

  17. Technical Manual for the SAM Biomass Power Generation Model

    SciTech Connect

    Jorgenson, J.; Gilman, P.; Dobos, A.

    2011-09-01

    This technical manual provides context for the implementation of the biomass electric power generation performance model in the National Renewable Energy Laboratory's (NREL's) System Advisor Model (SAM). Additionally, the report details the engineering and scientific principles behind the underlying calculations in the model. The framework established in this manual is designed to give users a complete understanding of behind-the-scenes calculations and the results generated.

  18. Detailed Physical Trough Model for NREL's Solar Advisor Model: Preprint

    SciTech Connect

    Wagner, M. J.; Blair, N.; Dobos, A.

    2010-10-01

    Solar Advisor Model (SAM) is a free software package made available by the National Renewable Energy Laboratory (NREL), Sandia National Laboratory, and the US Department of Energy. SAM contains hourly system performance and economic models for concentrating solar power (CSP) systems, photovoltaic, solar hot-water, and generic fuel-use technologies. Versions of SAM prior to 2010 included only the parabolic trough model based on Excelergy. This model uses top-level empirical performance curves to characterize plant behavior, and thus is limited in predictive capability for new technologies or component configurations. To address this and other functionality challenges, a new trough model; derived from physical first principles was commissioned to supplement the Excelergy-based empirical model. This new 'physical model' approaches the task of characterizing the performance of the whole parabolic trough plant by replacing empirical curve-fit relationships with more detailed calculations where practical. The resulting model matches the annual performance of the SAM empirical model (which has been previously verified with plant data) while maintaining run-times compatible with parametric analysis, adding additional flexibility in modeled system configurations, and providing more detailed performance calculations in the solar field, power block, piping, and storage subsystems.

  19. Reference Manual for the System Advisor Model's Wind Power Performance Model

    SciTech Connect

    Freeman, J.; Jorgenson, J.; Gilman, P.; Ferguson, T.

    2014-08-01

    This manual describes the National Renewable Energy Laboratory's System Advisor Model (SAM) wind power performance model. The model calculates the hourly electrical output of a single wind turbine or of a wind farm. The wind power performance model requires information about the wind resource, wind turbine specifications, wind farm layout (if applicable), and costs. In SAM, the performance model can be coupled to one of the financial models to calculate economic metrics for residential, commercial, or utility-scale wind projects. This manual describes the algorithms used by the wind power performance model, which is available in the SAM user interface and as part of the SAM Simulation Core (SSC) library, and is intended to supplement the user documentation that comes with the software.

  20. Incorporation of NREL Solar Advisor Model Photovoltaic Capabilities with GridLAB-D

    SciTech Connect

    Tuffner, Francis K.; Hammerstrom, Janelle L.; Singh, Ruchi

    2012-10-19

    This report provides a summary of the work updating the photovoltaic model inside GridLAB-D. The National Renewable Energy Laboratory Solar Advisor Model (SAM) was utilized as a basis for algorithms and validation of the new implementation. Subsequent testing revealed that the two implementations are nearly identical in both solar impacts and power output levels. This synergized model aides the system-level impact studies of GridLAB-D, but also allows more specific details of a particular site to be explored via the SAM software.

  1. ADVISOR: a systems analysis tool for advanced vehicle modeling

    NASA Astrophysics Data System (ADS)

    Markel, T.; Brooker, A.; Hendricks, T.; Johnson, V.; Kelly, K.; Kramer, B.; O'Keefe, M.; Sprik, S.; Wipke, K.

    This paper provides an overview of Advanced Vehicle Simulator (ADVISOR)—the US Department of Energy's (DOE's) ADVISOR written in the MATLAB/Simulink environment and developed by the National Renewable Energy Laboratory. ADVISOR provides the vehicle engineering community with an easy-to-use, flexible, yet robust and supported analysis package for advanced vehicle modeling. It is primarily used to quantify the fuel economy, the performance, and the emissions of vehicles that use alternative technologies including fuel cells, batteries, electric motors, and internal combustion engines in hybrid (i.e. multiple power sources) configurations. It excels at quantifying the relative change that can be expected due to the implementation of technology compared to a baseline scenario. ADVISOR's capabilities and limitations are presented and the power source models that are included in ADVISOR are discussed. Finally, several applications of the tool are presented to highlight ADVISOR's functionality. The content of this paper is based on a presentation made at the 'Development of Advanced Battery Engineering Models' workshop held in Crystal City, Virginia in August 2001.

  2. Integration, Validation, and Application of a PV Snow Coverage Model in SAM

    SciTech Connect

    Ryberg, David; Freeman, Janine

    2015-09-01

    Due to the increasing deployment of PV systems in snowy climates, there is significant interest in a method capable of estimating PV losses resulting from snow coverage that has been verified for a wide variety of system designs and locations. A scattering of independent snow coverage models have been developed over the last 15 years; however, there has been very little effort spent on verifying these models beyond the system design and location on which they were based. Moreover, none of the major PV modeling software products have incorporated any of these models into their workflow. In response to this deficiency, we have integrated the methodology of the snow model developed in the paper by Marion et al. [1] into the National Renewable Energy Laboratory's (NREL) System Advisor Model (SAM). In this work we describe how the snow model is implemented in SAM and discuss our demonstration of the model's effectiveness at reducing error in annual estimations for two PV arrays. Following this, we use this new functionality in conjunction with a long term historical dataset to estimate average snow losses across the United States for a typical PV system design. The open availability of the snow loss estimation capability in SAM to the PV modeling community, coupled with our results of the nation-wide study, will better equip the industry to accurately estimate PV energy production in areas affected by snowfall.

  3. Direct-Steam Linear Fresnel Performance Model for NREL's System Advisor Model

    SciTech Connect

    Wagner, M. J.; Zhu, G.

    2012-09-01

    This paper presents the technical formulation and demonstrated model performance results of a new direct-steam-generation (DSG) model in NREL's System Advisor Model (SAM). The model predicts the annual electricity production of a wide range of system configurations within the DSG Linear Fresnel technology by modeling hourly performance of the plant in detail. The quasi-steady-state formulation allows users to investigate energy and mass flows, operating temperatures, and pressure drops for geometries and solar field configurations of interest. The model includes tools for heat loss calculation using either empirical polynomial heat loss curves as a function of steam temperature, ambient temperature, and wind velocity, or a detailed evacuated tube receiver heat loss model. Thermal losses are evaluated using a computationally efficient nodal approach, where the solar field and headers are discretized into multiple nodes where heat losses, thermal inertia, steam conditions (including pressure, temperature, enthalpy, etc.) are individually evaluated during each time step of the simulation. This paper discusses the mathematical formulation for the solar field model and describes how the solar field is integrated with the other subsystem models, including the power cycle and optional auxiliary fossil system. Model results are also presented to demonstrate plant behavior in the various operating modes.

  4. P50/P90 Analysis for Solar Energy Systems Using the System Advisor Model: Preprint

    SciTech Connect

    Dobos, A. P.; Gilman, P.; Kasberg, M.

    2012-06-01

    To secure competitive financing for a solar energy generation project, the economic risk associated with interannual solar resource variability must be quantified. One way to quantify this risk is to calculate exceedance probabilities representing the amount of energy expected to be produced by a plant. Many years of solar radiation and metereological data are required to determine these values, often called P50 or P90 values for the level of certainty they represent. This paper describes the two methods implemented in the National Renewable Energy Laboratory's System Advisor Model (SAM) to calculate P50 and P90 exceedance probabilities for solar energy projects. The methodology and supporting data sets are applicable to photovoltaic, solar water heating, and concentrating solar power (CSP) systems.

  5. Modeling Photovoltaic Module-Level Power Electronics in the System Advisor Model; NREL (National Renewable Energy Laboratory)

    SciTech Connect

    2015-07-01

    Module-level power electronics, such as DC power optimizers, microinverters, and those found in AC modules, are increasing in popularity in smaller-scale photovoltaic (PV) systems as their prices continue to decline. Therefore, it is important to provide PV modelers with guidelines about how to model these distributed power electronics appropriately in PV modeling software. This paper extends the work completed at NREL that provided recommendations to model the performance of distributed power electronics in NREL’s popular PVWatts calculator [1], to provide similar guidelines for modeling these technologies in NREL's more complex System Advisor Model (SAM). Module-level power electronics - such as DC power optimizers, microinverters, and those found in AC modules-- are increasing in popularity in smaller-scale photovoltaic (PV) systems as their prices continue to decline. Therefore, it is important to provide PV modelers with guidelines about how to model these distributed power electronics appropriately in PV modeling software.

  6. The fSAM Model of False Recall

    ERIC Educational Resources Information Center

    Kimball, Daniel R.; Smith, Troy A.; Kahana, Michael J.

    2007-01-01

    The authors report a new theory of false memory building upon existing associative memory models and implemented in fSAM, the first fully specified quantitative model of false recall. Participants frequently intrude unstudied critical words while recalling lists comprising their strongest semantic associates but infrequently produce other…

  7. Introducing the aerosol-climate model MAECHAM5-SAM2

    NASA Astrophysics Data System (ADS)

    Hommel, R.; Timmreck, C.; Graf, H. F.

    2009-04-01

    We are presenting a new global aerosol model MAECHAM5-SAM2 to study the aerosol dynamics in the UTLS under background and volcanic conditions. The microphysical core modul SAM2 treats the formation, the evolution and the transport of stratospheric sulphuric acid aerosol. The aerosol size distribution and the weight percentage of the sulphuric acid solution is calculated dependent on the concentrations of H2SO4 and H2O, their vapor pressures, the atmospheric temperature and pressure. The fixed sectional method is used to resolve an aerosol distribution between 1 nm and 2.6 micron in particle radius. Homogeneous nucleation, condensation and evaporation, coagulation, water-vapor growth, sedimentation and sulphur chemistry are included. The module is applied in the middle-atmosphere MAECHAM5 model, resolving the atmosphere up to 0.01 hPa (~80 km) in 39 layers. It is shown here that MAECHAM5-SAM2 well represents in-situ measured size distributions of stratospheric background aerosol in the northern hemisphere mid-latitudes. Distinct differences can be seen when derived integrated aerosol parameters (surface area, effective radius) are compared with aerosol climatologies based on the SAGE II satellite instrument (derived by the University of Oxford and the NASA AMES laboratory). The bias between the model and the SAGE II data increases as the moment of the aerosol size distribution decreases. Thus the modeled effective radius show the strongest bias, followed by the aerosol surface area density. Correspondingly less biased are the higher moments volume area density and the mass density of the global stratospheric aerosol coverage. This finding supports the key finding No. 2 of the SPARC Assessment of Stratospheric Aerosol Properties (2006), where it was shown that during periods of very low aerosol load in the stratosphere, the consistency between in-situ and satellite measurements, which exist in a volcanically perturbed stratosphere, breaks down and significant

  8. Dynamic selection of models for a ventilator-management advisor.

    PubMed Central

    Rutledge, G. W.

    1993-01-01

    A ventilator-management advisor (VMA) is a computer program that monitors patients who are treated with a mechanical ventilator. A VMA implements a patient-specific physiologic model to interpret patient data and to predict the effects of alternative control settings for the ventilator. Because a VMA evaluates its physiologic model repeatedly during each cycle of data interpretation, highly complex models may require more computation time than is available in this time-critical application. On the other hand, less complex models may be inaccurate if they are unable to represent a patient's physiologic abnormalities. For each patient, a VMA should select a model that balances the tradeoff of prediction accuracy and computation-time complexity. I present a method to select models that are at an appropriate level of detail for time-constrained decision tasks. The method is based on a local search in a graph of models (GoM) for a model that maximizes the tradeoff of computation-time complexity and prediction accuracy. For each model under consideration, a belief network computes a probability of model adequacy given the qualitative prior information, and the goodness of fit of the model to the data provides a measure of the conditional probability of adequacy given the quantitative observations. I apply this method to the problem of model selection for a VMA. I describe an implementation of a graph of physiologic models that range in complexity from VentPlan, a simple model with 3 compartments, to VentSim, a multicompartment model with detailed airway, circulation and mechanical ventilator components.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:8130492

  9. A Spatial Analysis and Modeling System (SAMS) for environment management

    NASA Technical Reports Server (NTRS)

    Stetina, Fran; Hill, John; Chan, Paul; Jaske, Robert; Rochon, Gilbert

    1993-01-01

    This is a proposal to develop a uniform global environmental data gathering and distribution system to support the calibration and validation of remotely sensed data. SAMS is based on an enhanced version of FEMA's Integrated Emergency Management Information Systems and the Department of Defense's Air land Battlefield Environment Software Systems. This system consists of state-of-the-art graphics and visualization techniques, simulation models, database management and expert systems for conducting environmental and disaster preparedness studies. This software package will be integrated into various Landsat and UNEP-GRID stations which are planned to become direct readout stations during the EOS (Earth Observing System) timeframe. This system would be implemented as a pilot program to support the Tropical Rainfall Measuring Mission (TRMM). This will be a joint NASA-FEMA-University-Industry project.

  10. A Spatial Analysis and Modeling System (SAMS) for environment management

    NASA Technical Reports Server (NTRS)

    Vermillion, Charles H.; Stetina, Fran; Hill, John; Chan, Paul; Jaske, Robert; Rochon, Gilbert

    1992-01-01

    This is a proposal to develop a uniform global environmental data gathering and distribution system to support the calibration and validation of remotely sensed data. SAMS is based on an enhanced version of FE MA's Integrated Emergency Management Information Systems and the Department of Defense's Air Land Battlefield Environment Software Systems. This system consists of state-of-the-art graphics and visualization techniques, simulation models, database management and expert systems for conducting environmental and disaster preparedness studies. This software package will be integrated into various Landsat and UNEP-GRID stations which are planned to become direct readout stations during the EOS timeframe. This system would be implemented as a pilot program to support the Tropical Rainfall Measuring Mission (TRMM). This will be a joint NASA-FEMA-University-Industry project.

  11. The fSAM Model of False Recall

    PubMed Central

    Kimball, Daniel R.; Smith, Troy A.; Kahana, Michael J.

    2009-01-01

    The authors report a new theory of false memory building upon existing associative memory models and implemented in fSAM, the first fully specified quantitative model of false recall. Participants frequently intrude unstudied critical words while recalling lists comprising their strongest semantic associates but infrequently produce other extralist and prior-list intrusions. The authors developed the theory by simulating recall of such lists, using factorial combinations of semantic mechanisms operating at encoding, retrieval, or both stages. During encoding, unstudied words' associations to list context were strengthened in proportion to their strength of semantic association either to each studied word or to all co-rehearsed words. During retrieval, words received preference in proportion to their strength of semantic association to the most recently recalled single word or multiple words. The authors simulated all intrusion types and veridical recall for lists varying in semantic association strength among studied and critical words from the same and different lists. Multiplicative semantic encoding and retrieval mechanisms performed well in combination. Using such combined mechanisms, the authors also simulated several core findings from the Deese–Roediger–McDermott paradigm literature, including developmental patterns, specific list effects, association strength effects, and true–false correlations. These results challenge existing false-memory theories. PMID:17907869

  12. Solar Advisor Model; Session: Modeling and Analysis (Presentation)

    SciTech Connect

    Blair, N.

    2008-04-01

    This project supports the Solar America Initiative by: (1) providing a consistent framework for analyzing and comparing power system costs and performance across the range of solar technologies and markets, PV, solar heat systems, CSP, residential, commercial and utility markets; (2) developing and validating performance models to enable accurate calculation of levelized cost of energy (LCOE); (3) providing a consistent modeling platform for all TPP's; and (4) supporting implementation and usage of cost models.

  13. Application of the self-assembled monolayer (SAM) model to dithiophosphate and dithiocarbamate engine wear inhibitors

    SciTech Connect

    Zhou, Y.; Jiang, S.; Cagin, T.; Yamaguchi, E.S.; Frazier, R.; Ho, A.; Tang, Y.; Goddard, W.A. III

    2000-03-23

    In previous studies of dithiophosphate [TP=S{sub 2}P(OR){sub 2}] wear inhibitors bound to an oxidized iron surface, it was found that the cohesive energy of the self-assembled monolayers (SAM) for DTP molecules with various organic R groups correlate with the wear inhibition observed in full engine experiments. In this paper the calculations are expanded to consider dynamics at 500 K and the SAM model is used to predict new candidates for wear inhibitors. Using molecular dynamics (MD) simulations show that the SAM has one DTP per two surface Fe sites of iron oxide. At this coverage the cohesive energy of the SAM at 500 K is in the sequence 2-alkyl > 1-alkyl > aryl, which again correlates with wear inhibitor performance in engine wear tests. Dithiocarbamates are selected as the best candidate to supplement DTP.A number of possible alkyl substitutions for DTC were considered. The SAM model suggests that iC{sub 5} and nC{sub 3} are the best candidates, followed closely by iC{sub 3}.

  14. Construct Validation of the Louisiana School Analysis Model (SAM) Instructional Staff Questionnaire

    ERIC Educational Resources Information Center

    Bray-Clark, Nikki; Bates, Reid

    2005-01-01

    The purpose of this study was to validate the Louisiana SAM Instructional Staff Questionnaire, a key component of the Louisiana School Analysis Model. The model was designed as a comprehensive evaluation tool for schools. Principle axis factoring with oblique rotation was used to uncover the underlying structure of the SISQ. (Contains 1 table.)

  15. Evolution of SAM in an enhanced model for Monitoring WLCG services

    NASA Astrophysics Data System (ADS)

    Collados, David; Shade, John; Traylen (Cern, Steve; Imamagic (Srce, Emir

    2010-04-01

    It is four years now since the first prototypes of tools and tests started to monitor the Worldwide LHC Computing Grid (WLCG) services. One of these tools is the Service Availability Monitoring (SAM) framework, which superseded the SFT tool, and has become a keystone for the monthly WLCG availability and reliability computations. During this time, the grid has evolved into a robust, production-level infrastructure, in no small part thanks to the extensive monitoring infrastructure which includes testing, visualization and reporting. Experience gained with monitoring has led to emerging grid monitoring standards, and provided valuable input for the Operations Automation Strategy aimed at the regionalization of monitoring services. This change in scope, together with an ever-increasing number of services and infrastructures, make enhancements in the architecture of existing monitoring tools a necessity. This paper describes the present architecture of SAM, an enhanced and distributed model for monitoring WLCG services, and the required changes in SAM to adopt this new model inside the EGEE-III project.

  16. Results and Comparison from the SAM Linear Fresnel Technology Performance Model: Preprint

    SciTech Connect

    Wagner, M. J.

    2012-04-01

    This paper presents the new Linear Fresnel technology performance model in NREL's System Advisor Model. The model predicts the financial and technical performance of direct-steam-generation Linear Fresnel power plants, and can be used to analyze a range of system configurations. This paper presents a brief discussion of the model formulation and motivation, and provides extensive discussion of the model performance and financial results. The Linear Fresnel technology is also compared to other concentrating solar power technologies in both qualitative and quantitative measures. The Linear Fresnel model - developed in conjunction with the Electric Power Research Institute - provides users with the ability to model a variety of solar field layouts, fossil backup configurations, thermal receiver designs, and steam generation conditions. This flexibility aims to encompass current market solutions for the DSG Linear Fresnel technology, which is seeing increasing exposure in fossil plant augmentation and stand-alone power generation applications.

  17. Peer review presentation on systems performance modeling and solar advisor support.

    SciTech Connect

    Cameron, Christopher P.

    2010-05-01

    Accurate Performance Models are Critical to Project Development and Technology Evaluation - Accuracy and Uncertainty of Commonly-Used Models Unknown and Models Disagree. A Model Evaluation Process Has Been Developed with Industry, and High-Quality Weather and System Performance Data Sets Have Been Collected: (1) Evaluation is Underway using Residual Analysis of Hourly and Sub-Hourly Data for Clear and Diffuse Climates to Evaluate and Improve Models; and (2) Initial Results Have Been or Will Soon Be Presented at Key Conferences. Evaluation of Widely-Used Module, Inverter, and Irradiance Models, Including Those in SAM, PVWatts, and PVSyst, Will Be Completed This Year. Stochastic Modeling Has Been Performed to Support Reliability Task and Will Add Value to Parametric Analysis. An Industry Workshop will be Held This Fall To Review Results, Set Priorities. Support and Analysis has been Provided for TPP's, SETP, and PV Community. Goals for Future Work Include: (1) Improving Understanding of and Validating System Derate Factors; and (2) Developing a Dynamic Electrical Model of Arrays with Shaded or Mismatched Modules to Support Transient Analysis of Large Fields.

  18. SAM Technical Review Committee Final Report: Summary and Key Recommendations from the Onsite TRC Meeting Held April 22-23, 2013

    SciTech Connect

    Blair, N.; Dobos, S.; Janzou, S.; Gilman, P.; Freeman, J.; Kaffine, L.

    2013-08-01

    The System Advisor Model (SAM) is a broad and robust set of models and frameworks for analyzing both system performance and system financing. It does this across a range of technologies dominated by solar technologies including photovoltaics (PV) and concentrated solar power (CSP). The U.S. Department of Energy (DOE) Solar Energy Technology Program requested the SAM development team to review the photovoltaic performance modeling with the development community and specifically, with the independent engineering community. The report summarizes the major effort for this technical review committee (TRC).

  19. Senescence-accelerated mouse (SAM): a novel murine model of senescence.

    PubMed

    Takeda, T; Hosokawa, M; Higuchi, K

    1997-01-01

    The Senescence-Accelerated Mouse (SAM) has been under development by our research team at Kyoto University since 1970 through the selective inbreeding of the AKR/J strain of mice donated by the Jackson Laboratory in 1968, based on a graded score for senescence, life span, and pathologic phenotype. At present, there are 12 lines of SAM: nine senescence-prone inbred strains (SAMP) including SAMP1, SAMP2, SAMP3, SAMP6, SAMP7, SAMP8, SAMP9, SAMP10, and SAMP11; and three senescence-resistant inbred strains (SAMR) including SAMR1, SAMR4, and SAMR5. Data from survival curves, Gompertzian function, and grading score of senescence, together with growth patterns of body weight of these SAMP and SAMR, revealed that the characteristic feature of aging common to all SAMP mice is "accelerated senescence;" early onset and irreversible advance of senescence manifested by several signs and gross lesions such as the loss of normal behavior, various skin lesions, increased lordokyphosis, etc., after a period of normal development. In the course of SAM development, it became evident that SAMP strains manifest various pathologic phenotypes that are characteristic enough to differentiate the SAM strains. The genetic background and significance of SAM development are discussed. PMID:9088907

  20. The Advisor's Role

    ERIC Educational Resources Information Center

    Norris, Martha A.

    1975-01-01

    A training program for advisors is presented as a support system for implementing the Open Corridor concept into school systems. The following components of the advisor's role are discussed: selection and training, advisor-teacher relationship, work in the classroom, work with principals and parents, and role of teachers. (EC)

  1. Senescence-accelerated mouse (SAM) with special references to neurodegeneration models, SAMP8 and SAMP10 mice.

    PubMed

    Takeda, Toshio

    2009-04-01

    The SAM strains, a group of related inbred strains consisting of senescence-prone inbred strains (SAMP) and senescence-resistant inbred strains (SAMR), have been successfully developed by selective inbreeding of the AKR/J strain of mice donated by the Jackson laboratory in 1968. The characteristic feature of aging common to the SAMP and SAMR is accelerated senescence and normal aging, respectively. Furthermore, SAMP and SAMR strains of mice manifest various pathobiological phenotypes spontaneously. Among SAMP strains, SAMP8 and SAMP10 mice show age-related behavioral deterioration such as deficits in learning and memory, emotional disorders (reduced anxiety-like behavior and depressive behavior) and altered circadian rhythm associated with certain pathological, biochemical and pharmacological changes. Here, the previous and recent literature on SAM mice are reviewed with an emphasis on SAMP8 and SAMP10 mice. A spontaneous model like SAM with distinct advantages over the gene-modified model is hoped by investigators to be used more widely as a biogerontological resource to explore the etiopathogenesis of accelerated senescence and neurodegenerative disorders. PMID:19199030

  2. Senescence-accelerated mouse (SAM) as an animal model of senile dementia: pharmacological, neurochemical and molecular biological approach.

    PubMed

    Okuma, Y; Nomura, Y

    1998-12-01

    To elucidate the fundamental mechanism of age-related deficiencies of learning and to develop effective drugs for intervention in age-related diseases such as learning dysfunctions, pertinent animal models that have characteristics closely similar to human dysfunctions should be established. SAM (senescence-accelerated mouse) has been established as a murine model of the SAM strains, groups of related inbred strains including nine strains of accelerated senescence-prone, short-lived mice (SAMP) and three strains of accelerated senescence-resistant, long-lived mice (SAMR). SAMP-strain mice show relatively strain-specific age-associated phenotypic pathologies such as shortened life span and early manifestation of senescence. Among the SAMP-strain mice, SAMP8 mice show an age-related deterioration in learning ability. Here, the neuropathological, neurochemical and pharmacological features of SAM are reported, especially for SAMP8. Moreover, the effects of several drugs on the biochemical and behavioral alterations in SAMP8 and the etiologic manifestation of accelerated senescence are also discussed. PMID:9920195

  3. Building Capacity in a Rural North Carolina Community to Address Prostate Health Using a Lay Health Advisor Model.

    PubMed

    Vines, Anissa I; Hunter, Jaimie C; White, Brandolyn S; Richmond, Alan N

    2016-05-01

    Background Prostate cancer is a critical concern for African Americans in North Carolina (NC), and innovative strategies are needed to help rural African American men maximize their prostate health. Engaging the community in research affords opportunities to build capacity for teaching and raising awareness. Approach and Strategies A community steering committee of academicians, community partners, religious leaders, and other stakeholders modified a curriculum on prostate health and screening to include interactive knowledge- and skill-building activities. This curriculum was then used to train 15 African American lay health advisors, dubbed Prostate Cancer Ambassadors, in a rural NC community. Over the 2-day training, Ambassadors achieved statistically significant improvements in knowledge of prostate health and maintained confidence in teaching. The Ambassadors, in turn, used their personal networks to share their knowledge with over 1,000 individuals in their community. Finally, the Ambassadors became researchers, implementing a prostate health survey in local churches. Discussion and Conclusions It is feasible to use community engagement models for raising awareness of prostate health in NC African American communities. Mobilizing community coalitions to develop curricula ensures that the curricula meet the communities' needs, and training lay health advisors to deliver curricula helps secure community buy-in for the information. PMID:26232777

  4. Tracking the MSL-SAM methane detection source location Through Mars Regional Atmospheric Modeling System (MRAMS)

    NASA Astrophysics Data System (ADS)

    Pla-García, Jorge

    2016-04-01

    1. Introduction: The putative in situ detection of methane by Sample Analysis at Mars (SAM) instrument suite on Curiosi-ty at Gale crater has garnered significant attention because of the potential implications for the presence of geological methane sources or indigenous Martian organisms [1, 2]. SAM reported detection of back-ground levels of atmospheric methane of mean value 0.69±0.25 parts per billion by volume (ppbv) at the 95% confidence interval (CI). Additionally, in four sequential measurements spanning a 60-sol period, SAM observed elevated levels of methane of 7.2±2.1 ppbv (95% CI), implying that Mars is episodically producing methane from an additional unknown source. There are many major unresolved questions regard-ing this detection: 1) What are the potential sources of the methane release? 2) What causes the rapid decrease in concentration? and 3) Where is the re-lease location? 4) How spatially extensive is the re-lease? 5) For how long is CH4 released? Regarding the first question, the source of methane, is so far not identified. It could be related with geo-logical process like methane release from clathrates [3], serpentinisation [4] and volcanism [5]; or due to biological activity from methanogenesis [6]. To answer the second question, the rapid decrease in concentration, it is important to note that the photo-chemical lifetime of methane is of order 100 years, much longer than the atmospheric mixing time scale, and thus the gas should tend to be well mixed except near a source or shortly after an episodic release. The observed spike of 7 ppb from the background of <1 ppb, and then the rapid return to the background lev-el could be due to a sink (destruction) or due to at-mospheric mixing. A wind mediated erosion process of ordinary quartz crystals was proposed to produce activated quartz grains, which sequester methane by forming covalent Si-C bonds. If this process is op-erational on Mars today, which some recent prelimi-nary studies on

  5. Tracking the MSL-SAM methane detection source location Through Mars Regional Atmospheric Modeling System (MRAMS)

    NASA Astrophysics Data System (ADS)

    Pla-García, Jorge

    2016-04-01

    1. Introduction: The putative in situ detection of methane by Sample Analysis at Mars (SAM) instrument suite on Curiosi-ty at Gale crater has garnered significant attention because of the potential implications for the presence of geological methane sources or indigenous Martian organisms [1, 2]. SAM reported detection of back-ground levels of atmospheric methane of mean value 0.69±0.25 parts per billion by volume (ppbv) at the 95% confidence interval (CI). Additionally, in four sequential measurements spanning a 60-sol period, SAM observed elevated levels of methane of 7.2±2.1 ppbv (95% CI), implying that Mars is episodically producing methane from an additional unknown source. There are many major unresolved questions regard-ing this detection: 1) What are the potential sources of the methane release? 2) What causes the rapid decrease in concentration? and 3) Where is the re-lease location? 4) How spatially extensive is the re-lease? 5) For how long is CH4 released? Regarding the first question, the source of methane, is so far not identified. It could be related with geo-logical process like methane release from clathrates [3], serpentinisation [4] and volcanism [5]; or due to biological activity from methanogenesis [6]. To answer the second question, the rapid decrease in concentration, it is important to note that the photo-chemical lifetime of methane is of order 100 years, much longer than the atmospheric mixing time scale, and thus the gas should tend to be well mixed except near a source or shortly after an episodic release. The observed spike of 7 ppb from the background of <1 ppb, and then the rapid return to the background lev-el could be due to a sink (destruction) or due to at-mospheric mixing. A wind mediated erosion process of ordinary quartz crystals was proposed to produce activated quartz grains, which sequester methane by forming covalent Si-C bonds. If this process is op-erational on Mars today, which some recent prelimi-nary studies on

  6. Economic Analysis Case Studies of Battery Energy Storage with SAM

    SciTech Connect

    DiOrio, Nicholas; Dobos, Aron; Janzou, Steven

    2015-11-01

    Interest in energy storage has continued to increase as states like California have introduced mandates and subsidies to spur adoption. This energy storage includes customer sited behind-the-meter storage coupled with photovoltaics (PV). This paper presents case study results from California and Tennessee, which were performed to assess the economic benefit of customer-installed systems. Different dispatch strategies, including manual scheduling and automated peak-shaving were explored to determine ideal ways to use the storage system to increase the system value and mitigate demand charges. Incentives, complex electric tariffs, and site specific load and PV data were used to perform detailed analysis. The analysis was performed using the free, publically available System Advisor Model (SAM) tool. We find that installation of photovoltaics with a lithium-ion battery system priced at $300/kWh in Los Angeles under a high demand charge utility rate structure and dispatched using perfect day-ahead forecasting yields a positive net-present value, while all other scenarios cost the customer more than the savings accrued. Different dispatch strategies, including manual scheduling and automated peak-shaving were explored to determine ideal ways to use the storage system to increase the system value and mitigate demand charges. Incentives, complex electric tariffs, and site specific load and PV data were used to perform detailed analysis. The analysis was performed using the free, publically available System Advisor Model (SAM) tool. We find that installation of photovoltaics with a lithium-ion battery system priced at $300/kWh in Los Angeles under a high demand charge utility rate structure and dispatched using perfect day-ahead forecasting yields a positive net-present value, while all other scenarios cost the customer more than the savings accrued.

  7. ReefSAM - Reef Sedimentary Accretion Model: A new 3D coral reef evolution model/simulator

    NASA Astrophysics Data System (ADS)

    Barrett, Samuel; Webster, Jody

    2013-04-01

    Coral reefs show characteristic morphological patterns (e.g. coral dominated margins with detrital carbonate dominated lagoons/back-reef) and temporal development (e.g. Hopley et al. 2007). While the processes which lead to predictable patterns on a range of scales have been discussed qualitatively, a full quantitative understanding of the range of processes and parameters involved requires modelling. Previous attempts to model complex Holocene reef systems (i.e. One Tree Reef, GBR - Barrett and Webster 2012) using a carbonate stratigraphic forward model (Carbonate3D - Warrlich et al. 2002) identified a number of important but unsimulated processes and potential model improvements. ReefSAM has been written from scratch in Matlab using these findings and experiences from using Carbonate3D. It simulates coralgal accretion and carbonate sand production and transport. Specific improvements include: 1. a more complex hydrodynamic model based on wave refraction and incorporating vertical (depth) and lateral (substrate dependent) variations in transport energy and erosion. 2. a complex reef growth model incorporating depth, wave energy/turbidity and substrate composition. 3. Paleo-water depth, paleo-wave energy and bio-zone (combination of paleo-water depth and wave energy) model outputs allowing coralgal habitat changes through time and space to be simulated and compared to observational data. The model is compared to the well studied One Tree Reef - tests similar to those undertaken in Barrett and Webster 2012 with Carbonate3D are presented. Model development coincides with plans for further intensive drilling at One Tree Reef (mid 2013) providing an opportunity to test the model predictively. The model is still in active development. References: Barrett, S.J., Webster, J.M.,2012. Holocene evolution of the Great Barrier Reef: Insights from 3D numerical modelling. Sedimentary Geology 265-266, 56-71. Warrlich, G.M.D., Waltham, D.A., Bosence D.W.J., 2002. Quantifying the

  8. The SAM software system for modeling severe accidents at nuclear power plants equipped with VVER reactors on full-scale and analytic training simulators

    NASA Astrophysics Data System (ADS)

    Osadchaya, D. Yu.; Fuks, R. L.

    2014-04-01

    The architecture of the SAM software package intended for modeling beyond-design-basis accidents at nuclear power plants equipped with VVER reactors evolving into a severe stage with core melting and failure of the reactor pressure vessel is presented. By using the SAM software package it is possible to perform comprehensive modeling of the entire emergency process from the failure initiating event to the stage of severe accident involving meltdown of nuclear fuel, failure of the reactor pressure vessel, and escape of corium onto the concrete basement or into the corium catcher with retention of molten products in it.

  9. Pilot weather advisor

    NASA Technical Reports Server (NTRS)

    Kilgore, W. A.; Seth, S.; Crabill, N. L.; Shipley, S. T.; Graffman, I.; Oneill, J.

    1992-01-01

    The results of the work performed by ViGYAN, Inc., to demonstrate the Pilot Weather Advisor cockpit weather data system using a broadcast satellite communication system are presented. The Pilot Weather Advisor demonstrated that the technical problems involved with transmitting significant amount of weather data to an aircraft in-flight or on-the-ground via satellite are solvable with today's technology. The Pilot Weather Advisor appears to be a viable solution for providing accurate and timely weather information for general aviation aircraft.

  10. MyFoodAdvisor

    MedlinePlus

    ... Password? Login Cancel Can I eat this? . . . Meal Planning and Tips Managing diabetes is a challenge that ... can make it easier. Discover more about meal planning options and how MyFoodAdvisor can help. Explore Foods ...

  11. Outcome Effectiveness of the Lay Health Advisor Model among Latinos in the United States: An Examination by Role

    ERIC Educational Resources Information Center

    Ayala, Guadalupe X.; Vaz, Lara; Earp, Jo Anne; Elder, John P.; Cherrington, Andrea

    2010-01-01

    The objective of this study was to examine lay health advisor (LHA) programs designed to promote health among US Latinos and the extent to which educator-only versus educator-plus-bridge programs differed in designs and outcomes achieved. Two independent coders reviewed 128 published articles on LHAs yielding information at two levels: (i) study…

  12. SAP Minutes No.2015-03 for FIFRA meeting held 9/15-17/2015. A set of scientific issues being considered by the Environmental Protection Agency regarding development of a spatial aquatic model(SAM)for pesticide risk assessment

    Technology Transfer Automated Retrieval System (TEKTRAN)

    On September 15-17th, 2014, the US Environmental Protection Agency convened a public meeting of the FIFRA Scientific Advisory Panel (SAP) to address scientific issues associated with the agency’s “Development of a Spatial Aquatic Model (SAM) for Pesticide Risk Assessment”. The goal of SAM is to impr...

  13. Online Instructors as Thinking Advisors: A Model for Online Learner Adaptation

    ERIC Educational Resources Information Center

    Benedetti, Christopher

    2015-01-01

    This article examines the characteristics and challenges of online instruction and presents a model for improving learner adaptation in an online classroom. Instruction in an online classroom presents many challenges, including learner individualization. Individual differences in learning styles and preferences are often not considered in the…

  14. Heterotypic Sam-Sam association between Odin-Sam1 and Arap3-Sam: binding affinity and structural insights

    PubMed Central

    Mercurio, Flavia A.; Marasco, Daniela; Pirone, Luciano; Scognamiglio, Pasqualina L.; Pedone, Emilia M.; Pellecchia, Maurizio

    2013-01-01

    Arap3 is a phosphatidylinositol 3 kinase effector protein that plays a role as GTP-ase activator (GAP) for Arf6 and RhoA. Arap3 contains a sterile alpha motif (Sam) domain that presents high sequence homology with the Sam domain of the EphA2-receptor (EphA2-Sam); both Arap3-Sam and EphA2-Sam are able to associate with the Sam domain of the lipid phosphatase Ship2 (Ship2-Sam). Recently, we have reported on a novel interaction between the first Sam domain of Odin (Odin-Sam1), a protein belonging to the ANKS (ANKyrin repeat and Sam domain containing) family, and EphA2-Sam. In the current work we apply Nuclear Magnetic Resonance (NMR) spectroscopy, Surface Plasmon Resonance (SPR) and Isothermal Titration Calorimetry (ITC) to characterize the association between Arap3-Sam and Odin-Sam1. We show that these two Sam domains interact with low micromolar affinity. Moreover, by means of molecular docking techniques, supported by NMR data, we demonstrate that Odin-Sam1 and Arap3-Sam may bind with a topology that is common to several Sam-Sam complexes. The unveiled structural details form the basis for the design of potential peptide-antagonists, that could be used as chemical tools to investigate functional aspects related to heterotypic Arap3-Sam associations. PMID:23239578

  15. Academic Advisors: The Boundary Spanners.

    ERIC Educational Resources Information Center

    Fitzgerald, Laurine E.

    Perspectives on the roles of academic advisors are considered. It is suggested that academic advisors are often "boundary spanners," those who participate in two or more aspects of the activities of the institution. Academic advisors' functions cut across multidisciplinary lines, affecting curricular decisions and curriculum development, career…

  16. Advisors as Interaction Designers

    ERIC Educational Resources Information Center

    Shockley-Zalabak, Pamela

    2012-01-01

    Pamela Shockley-Zalabak, Chancellor, University of Colorado Colorado Springs, gave the following speech at the 2011 NACADA Annual Conference October 3, 2011, at the Colorado Convention Center in Denver. She provides an overview of changes affecting U.S. higher education, commentary on how those changes affect the role of the academic advisor, and…

  17. Critics and advisors: Heuristic knowledge and manufacturability

    SciTech Connect

    Rivera, J.J.; Stubblefield, W.A.; Ames, A.L.

    1996-06-01

    In recent years, much of the progress in Computer-Aided Manufacturing has emphasized the use of simulation, finite-element analysis, and other science-based techniques to plan and evaluate manufacturing processes. These approaches are all based on the idea that we can build sufficiently faithful models of complex manufacturing processes such as machining, welding, and casting. Although there has been considerable progress in this area, it continues to suffer from difficulties: the first of these is that the kind of highly accurate models that this approach requires may take many person months to construct, and the second is the large amount of computing resources needed to run these simulations. Two design advisors, Near Net-Shape Advisor and Design for Machinability Advisor, are being developed to explore the role of heuristic, knowledge-based systems for manufacturing processes, both as an alternative to more analytical techniques, and also in support of these techniques. Currently the advisors are both in the prototype stage. All indications lead to the conclusion that the advisors will be successful and lay the groundwork for additional systems such as these in the future.

  18. Synthia Tonn, SAM Engineer

    NASA Video Gallery

    The Sample Analysis at Mars (SAM) is a suite of instruments developed for use on the Mars Science Laboratory, designed to help find out whether or not Mars ever supported life. This video profiles ...

  19. The Marshall Engineering Thermosphere model atmosphere Statistical Analysis Mode (MET-SAM)

    NASA Technical Reports Server (NTRS)

    Smith, Robert E.; Catlett, Karen; Anderson, B. Jeffrey

    1991-01-01

    The minimum, mean, and maximum exospheric temperature on the globe were calculated for every three hour period from 1947 through 1989 using the algorithms in the Marshall Engineering Thermosphere (MET) model and the appropriate solar activity input parameters. Cumulative percent frequency (CPF) distributions were then calculated for each of these temperatures at five levels of solar activity as defined by the 13-month smoothed values of the 10.7-cm solar radio noise flux. Next, the 50, 95, 97.7, and 100 percentile temperature values in each of these five levels of solar activity were curve fit as a function of the 13-month smoothed 10.7-cm flux. The resulting algorithms are used to compute the exospheric temperature in the MET model instead of the technique developed by Jacchia in his 1970 model. These temperatures are then used to enter tables to determine the total mass density and/or the atomic oxygen number density for application to engineering problems. Users can specify the risk level they are willing to accept in the results of analyses that require neutral atmosphere parameters inputs. The model eliminates the guess work in how to combine the solar activity input parameters to insure that the results provide answers at the proper risk levels.

  20. Training Public Health Advisors

    PubMed Central

    Meyer, Pamela A.; Brusuelas, Kristin M.; Baden, Daniel J.; Duncan, Heather L.

    2015-01-01

    Federal public health advisors provide guidance and assistance to health departments to improve public health program work. The Centers for Disease Control and Prevention (CDC) prepares them with specialized training in administering public health programs. This article describes the evolving training and is based on internal CDC documents and interviews. The first federal public health advisors worked in health departments to assist with controlling syphilis after World War II. Over time, more CDC prevention programs hired them. To meet emerging needs, 3 major changes occurred: the Public Health Prevention Service, a fellowship program, in 1999; the Public Health Associate Program in 2007; and integration of those programs. Key components of the updated training are competency-based training, field experience, supervision, recruitment and retention, and stakeholder support. The enduring strength of the training has been the experience in a public health agency developing practical skills for program implementation and management. PMID:25564995

  1. Training Public Health Advisors.

    PubMed

    Meyer, Pamela A; Brusuelas, Kristin M; Baden, Daniel J; Duncan, Heather L

    2015-01-01

    Federal public health advisors provide guidance and assistance to health departments to improve public health program work. The Centers for Disease Control and Prevention (CDC) prepares them with specialized training in administering public health programs. This article describes the evolving training and is based on internal CDC documents and interviews. The first federal public health advisors worked in health departments to assist with controlling syphilis after World War II. Over time, more CDC prevention programs hired them. To meet emerging needs, 3 major changes occurred: the Public Health Prevention Service, a fellowship program, in 1999; the Public Health Associate Program in 2007; and integration of those programs. Key components of the updated training are competency-based training, field experience, supervision, recruitment and retention, and stakeholder support. The enduring strength of the training has been the experience in a public health agency developing practical skills for program implementation and management. PMID:25564995

  2. Fast and slow responses of Southern Ocean sea surface temperature to SAM in coupled climate models

    NASA Astrophysics Data System (ADS)

    Krishnamurti, T. N.; Kumar, Vinay; Simon, Anu; Thomas, Aype; Bhardwaj, Amit; Das, Sweta; Senroy, Soma; Roy Bhowmik, S. K.

    2016-06-01

    A major rain storm in Uttarakhand (India) caused heavy rains and major loss of life from floods and land slide during 16-18 June, 2013. The observed daily maximum rainfall rates (3-hourly) during the 16th and 17th June were 220 and 340 mm respectively. This event is addressed via sensitivity studies using a cloud resolving non-hydrostatic model with detailed microphysics. The streaming of moist air from the east-south-east and warmer air from the south-west contributed to the sustained large population and amplitude of buoyancy and the associated CAPE contributed to the longer period of heavy rains. This study addresses the concept of Buoyancy as a means for large vertical accelerations, stronger vertical motions, extreme rain rates and the mechanisms that relate to the time rates of change. A post-processing algorithm provides an analysis of time rate of change for the buoyancy. Moist air streams and warm/moist air intrusions into heavily raining clouds are part of this buoyancy enhancement framework. Improvements in modeling of the extreme rain event came from adaptive observational strategy that showed lack of moisture data sets in these vital regions. We show that a moist boundary layer near the Bay of Bengal leads to moist rivers of moisture where the horizontal convergence confines a large population of buoyancy elements with large magnitudes of buoyancy that streams towards the region of extreme orographic rains. The areas covered in this study include: (i) Use of high resolution cloud modeling (1-km), (ii) Now casting of rains using physical initialization with a Newtonian relaxation, (iii) Use of an adaptive observational strategy, (iii) Sensitivity of the evolution of fields and population of buoyancy elements to boundary layer moisture, (iv) Role of orography and details of buoyancy budget.

  3. Hydroforming design and process advisor

    SciTech Connect

    Greer, J.T.; Ni, C.M.

    1996-10-10

    The hydroforming process involves hydraulically forming components by conforming them to the inner contours of a die. These contours can be complex and can often cause the material being formed to be stressed to rupture. Considerable process knowledge and materials modeling expertise is required to design hydroform dies and hydroformed parts that are readily formed without being overly stressed. For this CRADA, materials properties for steel tubes subjected to hydraulic stresses were collected; algorithms were developed which combined the materials properties data with process knowledge; and a user friendly graphical interface was utilized to make the system usable by a design engineer. A prototype hydroforming advisor was completed and delivered to GM. The technical objectives of the CRADA were met allowing for the development of an intelligent design systems, prediction of forming properties related to hydroforming, simulation and modeling of process execution, and design optimization. The design advisor allows a rapid and seamless approach to integration an otherwise enormous and onerous task of analysis and evaluation.

  4. The senescence-accelerated mouse (SAM): a higher oxidative stress and age-dependent degenerative diseases model.

    PubMed

    Chiba, Yoichi; Shimada, Atsuyoshi; Kumagai, Naoko; Yoshikawa, Keisuke; Ishii, Sanae; Furukawa, Ayako; Takei, Shiro; Sakura, Masaaki; Kawamura, Noriko; Hosokawa, Masanori

    2009-04-01

    The SAM strain of mice is actually a group of related inbred strains consisting of a series of SAMP (accelerated senescence-prone) and SAMR (accelerated senescence-resistant) strains. Compared with the SAMR strains, the SAMP strains show a more accelerated senescence process, a shorter lifespan, and an earlier onset and more rapid progress of age-associated pathological phenotypes similar to human geriatric disorders. The higher oxidative stress status observed in SAMP mice is partly caused by mitochondrial dysfunction, and may be a cause of this senescence acceleration and age-dependent alterations in cell structure and function. Based on our recent observations, we discuss a possible mechanism for mitochondrial dysfunction resulting in the excessive production of reactive oxygen species, and a role for the hyperoxidative stress status in neurodegeneration in SAMP mice. These SAM strains can serve as a useful tool to understand the cellular mechanisms of age-dependent degeneration, and to develop clinical interventions. PMID:18688709

  5. Pilot Weather Advisor System

    NASA Technical Reports Server (NTRS)

    Lindamood, Glenn; Martzaklis, Konstantinos Gus; Hoffler, Keith; Hill, Damon; Mehrotra, Sudhir C.; White, E. Richard; Fisher, Bruce D.; Crabill, Norman L.; Tucholski, Allen D.

    2006-01-01

    The Pilot Weather Advisor (PWA) system is an automated satellite radio-broadcasting system that provides nearly real-time weather data to pilots of aircraft in flight anywhere in the continental United States. The system was designed to enhance safety in two distinct ways: First, the automated receipt of information would relieve the pilot of the time-consuming and distracting task of obtaining weather information via voice communication with ground stations. Second, the presentation of the information would be centered around a map format, thereby making the spatial and temporal relationships in the surrounding weather situation much easier to understand

  6. First aid advisor

    SciTech Connect

    Saffer, S.I.; Weng, Wen-Chang

    1996-12-31

    A knowledge-based system (KBS), First Aid Advisor (FAA), is developed to provide a guidance for either a trained or untrained person to take some emergent actions to rescue the victim from life-threatening hazard or to prevent from causing serious problem before the Emergency Medical Service System (EMS) personnels arrive the scene. The First Aid Advisor will collect victim`s information by interacting with the user through a sequence of questions, analyze the victim`s problem, and give instructions step by step before offering a final advice. Since all the possible conditions are taken into consideration, it will guide the user to perform first aid from the most to the least urgent step for the victim. Furthermore, it will offer a suitable first aid advice for victim and eliminate the possibility of wrong actions done by trained person due to carelessness or nervousness. Offering advice to handle problems involving life-threatening conditions is the main objective of this system. In this paper, we will describe six major components of FAA and their respective tasks. Decision tables and dependency diagrams used in FAA implementation will also be described. System performance issues will conclude the paper.

  7. Outcome effectiveness of the lay health advisor model among Latinos in the United States: an examination by role

    PubMed Central

    Ayala, Guadalupe X.; Vaz, Lara; Earp, Jo Anne; Elder, John P.; Cherrington, Andrea

    2010-01-01

    The objective of this study was to examine lay health advisor (LHA) programs designed to promote health among US Latinos and the extent to which educator-only versus educator-plus-bridge programs differed in designs and outcomes achieved. Two independent coders reviewed 128 published articles on LHAs yielding information at two levels: (i) study design and participant and LHA characteristics from 61 studies that broadly compared educator-only versus educator-plus-bridge programs and (ii) implementation features and outcomes from 17 randomized controlled trials or quasi-experimental studies with outcome data. LHA programs have been widely used with Latinos in certain US regions; our findings indicate that LHAs are effective intervention agents. We identified differences between educator-only and educator-plus-bridge LHA programs, although the small number of educator-plus-bridge programs with outcome data limited comparisons. Major gaps remain in research targeting Latino subgroups other than Mexican immigrants/Mexican Americans. Sufficient research has evaluated LHA programs among Latinos on their ability to achieve health behavior and/or health status changes. In the future, more of a focus on organizational and policy changes is warranted. Questions remain about diversity in LHAs’ characteristics and roles, which influence not only outcomes but also program sustainability and dissemination. PMID:20603384

  8. The Traffic Management Advisor

    NASA Technical Reports Server (NTRS)

    Nedell, William; Erzberger, Heinz; Neuman, Frank

    1990-01-01

    The traffic management advisor (TMA) is comprised of algorithms, a graphical interface, and interactive tools for controlling the flow of air traffic into the terminal area. The primary algorithm incorporated in it is a real-time scheduler which generates efficient landing sequences and landing times for arrivals within about 200 n.m. from touchdown. A unique feature of the TMA is its graphical interface that allows the traffic manager to modify the computer-generated schedules for specific aircraft while allowing the automatic scheduler to continue generating schedules for all other aircraft. The graphical interface also provides convenient methods for monitoring the traffic flow and changing scheduling parameters during real-time operation.

  9. The Building Design Advisor

    SciTech Connect

    Papamichael, K.; LaPorta, J.; Chauvet, H.; Collins, D.; Trzcinski, T.; Thorpe, J.; Selkowitz, S.

    1996-03-01

    The Building Design Advisor (BDA) is a software environment that supports the integrated use of multiple analysis and visualization tools throughout the building design process, from the initial, schematic design phases to the detailed specification of building components and systems. Based on a comprehensive design theory, the BDA uses an object-oriented representation of the building and its context, and acts as a data manager and process controller to allow building designers to quickly navigate through the multitude of descriptive and performance parameters addressed by the analysis and visualization tools linked to the BDA. Through the Browser the user can edit the values of input parameters and select any number of input and/or output parameters for display in the Decision Desktop. The Desktop allows building designers to compare multiple design alternatives with respect to any number of parameters addressed by the tools linked to the BDA.

  10. Safety review advisor

    SciTech Connect

    Boshers, J.A.; Alguindigue, I.E.; Uhrig, R.E. . Dept. of Nuclear Engineering); Burnett, C.G. )

    1989-01-01

    The University of Tennessee's Nuclear Engineering Department, in cooperation with the Tennessee Valley Authority (TVA), is evaluating the feasibility of utilizing an expert system to aid in 10CFR50.59 evaluations. This paper discusses the history of 10CFR50.59 reviews, and details the development approach used in the construction of a prototype Safety Review Advisor (SRA). The goals for this expert system prototype are to (1) aid the engineer in the evaluation process by directing his attention to the appropriate critical issues, (2) increase the efficiency, consistency, and thoroughness of the evaluation process, and (3) provide a foundation of appropriate Safety Analysis Report (SAR) references for the reviewer. 6 refs., 2 figs.

  11. The Advisor Quality Survey: Good College Advisors Are Available, Knowledgeable, and Autonomy Supportive

    ERIC Educational Resources Information Center

    Sheldon, Kennon M.; Garton, Bryan; Orr, Rachael; Smith, Amy

    2015-01-01

    Most US institutions of higher education do not assess advisor quality. We report a scale development effort informed by the developmental prescriptions of self-determination theory (Deci & Ryan, 1985, 2000). The 15-item Missouri Advisor Quality Survey assesses advisor knowledge, advisor availability, and advisor autonomy supportiveness.…

  12. Inverse modeling in magnetic source imaging: Comparison of MUSIC, SAM(g2), and sLORETA to interictal intracranial EEG.

    PubMed

    de Gooijer-van de Groep, Karin L; Leijten, Frans S S; Ferrier, Cyrille H; Huiskamp, Geertjan J M

    2013-09-01

    Magnetoencephalography (MEG) is used in the presurgical work-up of patients with focal epilepsy. In particular, localization of MEG interictal spikes may guide or replace invasive electroencephalography monitoring that is required in difficult cases. From literature, it is not clear which MEG source localization method performs best in this clinical setting. Therefore, we applied three source localization methods to the same data from a large patient group for which a gold standard, interictal spikes as identified in electrocorticography (ECoG), was available. The methods used were multiple signal classification (MUSIC), Synthetic Aperture Magnetometry kurtosis [SAM(g2)], and standardized low-resolution electromagnetic tomography. MEG and ECoG data from 38 patients with refractory focal epilepsy were obtained. Results of the three source localization methods applied to the interictal MEG data were assigned to predefined anatomical regions. Interictal spikes as identified in ECoG were also assigned to these regions. Identified regions by each MEG method were compared to ECoG. Sensitivity and positive predictive value (PPV) of each MEG method were calculated. All three MEG methods showed a similar overall correlate with ECoG spikes, but the methods differ in which regions they detect. The choice of the inverse model thus has an unexpected influence on the results of magnetic source imaging. Combining inverse methods and seeking consensus can be used to improve specificity at the cost of some sensitivity. Combining MUSIC with SAM(g2) gives the best results (sensitivity = 38% and PPV = 82%). PMID:22431346

  13. Common themes and differences in SAM recognition among SAM riboswitches

    PubMed Central

    Price, Ian R.; Grigg, Jason C.; Ke, Ailong

    2014-01-01

    The recent discovery of short cis-acting RNA elements termed riboswitches has caused a paradigm shift in our understanding of genetic regulatory mechanisms. The three distinct superfamilies of S-adenosyl-L-methionine (SAM) riboswitches are the most commonly found riboswitch classes in nature. These RNAs represent three independent evolutionary solutions to achieve specific SAM recognition. This review summarizes research on 1) modes of gene regulatory mechanisms, 2) common themes and differences in ligand recognition, and 3) ligand-induced conformational dynamics among SAM riboswitch families. The body of work on the SAM riboswitch families constitutes a useful primer to the topic of gene regulatory RNAs as a whole. PMID:24863160

  14. Spot and Runway Departure Advisor

    NASA Technical Reports Server (NTRS)

    Jung, Yoon Chul

    2013-01-01

    The Spot and Runway Departure Advisor (SARDA) is a research prototype of a decision support tool for ATC tower controllers to assist in manging and controlling traffic on the surface of an airport. SARDA employs a scheduler to generate an optimal runway schedule and gate push-back - spot release sequence and schedule that improves efficiency of surface operations. The advisories for ATC tower controllers are displayed on an Electronic Flight Strip (EFS) system. The human-in-the-loop simulation of the SARDA tool was conducted for east operations of Dallas-Ft. Worth International Airport (DFW) to evaluate performance of the SARDA tool and human factors, such as situational awareness and workload. The results indicates noticeable taxi delay reduction and fuel savings by using the SARDA tool. Reduction in controller workload were also observed throughout the scenario runs. The future plan includes modeling and simulation of the ramp operations of the Charlotte International Airport, and develop a decision support tool for the ramp controllers.

  15. Greek Advisors' Perceptions of Sorority Hazing.

    ERIC Educational Resources Information Center

    Shaw, Deborah Lee; Morgan, Thomas E.

    1990-01-01

    Examined Greek advisors' opinions on several issues related to sorority hazing. Results from 283 members of the Association of Fraternity Advisors revealed that, although almost all campuses had a hazing policy, over half of advisors felt that hazing still existed in some of their groups. Policies for handling hazing incidents were varied.…

  16. Structural insights into SAM domain-mediated tankyrase oligomerization.

    PubMed

    DaRosa, Paul A; Ovchinnikov, Sergey; Xu, Wenqing; Klevit, Rachel E

    2016-09-01

    Tankyrase 1 (TNKS1; a.k.a. ARTD5) and tankyrase 2 (TNKS2; a.k.a ARTD6) are highly homologous poly(ADP-ribose) polymerases (PARPs) that function in a wide variety of cellular processes including Wnt signaling, Src signaling, Akt signaling, Glut4 vesicle translocation, telomere length regulation, and centriole and spindle pole maturation. Tankyrase proteins include a sterile alpha motif (SAM) domain that undergoes oligomerization in vitro and in vivo. However, the SAM domains of TNKS1 and TNKS2 have not been structurally characterized and the mode of oligomerization is not yet defined. Here we model the SAM domain-mediated oligomerization of tankyrase. The structural model, supported by mutagenesis and NMR analysis, demonstrates a helical, homotypic head-to-tail polymer that facilitates TNKS self-association. Furthermore, we show that TNKS1 and TNKS2 can form (TNKS1 SAM-TNKS2 SAM) hetero-oligomeric structures mediated by their SAM domains. Though wild-type tankyrase proteins have very low solubility, model-based mutations of the SAM oligomerization interface residues allowed us to obtain soluble TNKS proteins. These structural insights will be invaluable for the functional and biophysical characterization of TNKS1/2, including the role of TNKS oligomerization in protein poly(ADP-ribosyl)ation (PARylation) and PARylation-dependent ubiquitylation. PMID:27328430

  17. Feature-Based Measuring Advisor, Version 2.1

    SciTech Connect

    Brown, Curtis

    2002-07-22

    The purpose of Feature-Based Measuring Advisor (FBMeas Advisor) is a graphical user interface enabling users to more effectively interact with a solid model for measuring applications. Specifically, the FBMeas Advisor provides a user interface to the functions and capabilities of the FBMeas component technology. The FBMeas Advisor allows end users to visualize, organize, and document their measuring process plans and to facilitate the generation of a coordinate measuring machine part programs before a workpiece is fabricated. The FBMeas Advisor permits the user to interact with an FBTol-enabled solid model to interactively and/or automatically create a set of measuring features (e.g., cylinders, planes, slots) that comply with national and international interface standards (i.e., DMIS (Dimensional Measuring Interface Standard, ISO 22063)). Measuring plans include defining measuring features interactively or automatically. Associating measuring features with appropriate tolerances and datum features via FBTol. Determining what measuring tasks (e.g., measure this feature to validate a size tolerance, measure this feature to establish a primary datum for a specific datum reference frame) are necessary to measure the feature. Assigning a measuring method to perform the task. For coordinate metrology measuring methods, measure points are determined interactively or automatically. Sensors and sensor configurations are defined, represented, and assigned to measuring methods. Measuring machines are defined, represented, and assigned to measure operations. Upon completion of a coordinate metrology measuring plan, FBMeas allows DMIS code segments to be generated, which can be used for DMIS-based CMM part programs.

  18. Faces of Marshall: Sam Ortega

    NASA Video Gallery

    Several Marshall employees were interviewed as part of Marshall's 50th Anniversary activities. Engineer Sam Ortega tells his story of how he came to work as an engineer at Marshall and how sewing a...

  19. SAM II Data and Information

    Atmospheric Science Data Center

    2016-07-06

    ... Data obtained from the Stratospheric Aerosol Measurement (SAM) II instrument, which flew on board the Nimbus-7 ... Spatial Resolution:  The altitude profiles of aerosol extinction have a 1 km vertical resolution. Temporal ...

  20. The entomologist as a science partner and curriculum advisor: The Earth School model for grades 6--8

    NASA Astrophysics Data System (ADS)

    Marshall, Bethany Johnston

    The Earth School model for creation of partnerships between university scientists and public schools began with a traditional research project involving the study of macroinvertebrate recolonization of agriculturally based restored wetlands. From fieldwork designed to address hypotheses of community composition over time, protocols and equipment evolved for application in middle-school classrooms. In addition to classroom teachers guiding their students in replicating active scientific research, the inclusion of a science partner was key to the success of this model. To ensure that the classroom teachers were themselves comfortable as researchers, monthly staff development workshops were conducted as a component of the Earth School model. The use of entomology as a unifying theme for educational scientific investigation lets the student explore virtually every other system in the biosphere. Because of the unparalleled survivability and adaptability of insects, we can find examples from all biomes, all time references and all disciplines. Over the course of long-term continuous exploration, learners become familiar with relationships and patterns evident in natural situations. These same patterns of birth, growth and decay are much more vividly demonstrated in the field than in textbooks. Similarly, concrete examples of feeding relationships between organisms are plentiful in nearly any outdoor situation. The following model incorporates current research from multiple scientific disciplines but focuses on the many and varied research activities offered by the entomological community. Teachers and students in a primarily urban setting made extensive use of the materials developed through the course of this model's development. Their feedback as the materials were integrated into an established curriculum allowed for the fine-tuning of activity development. A conversion template has evolved that gives teachers, curriculum directors, parents and other educators a

  1. Stability of mixed PEO-thiol SAMs for biosensing applications.

    PubMed

    Jans, Karolien; Bonroy, Kristien; De Palma, Randy; Reekmans, Gunter; Jans, Hilde; Laureyn, Wim; Smet, Mario; Borghs, Gustaaf; Maes, Guido

    2008-04-15

    The secret of a successful affinity biosensor partially hides in the chemical interface layer between the transducer system and the biological receptor molecules. Over the past decade, several methodologies for the construction of such interface layers have been developed on the basis of the deposition of self-assembled monolayers (SAMs) of alkanethiols on gold. Moreover, mixed SAMs of polyethylene oxide (PEO) containing thiols have been applied for the immobilization of biological receptors. Despite the intense research in the field of thiol SAMs, relatively little is known about their biosensing properties in correlation with their long-term stability. Especially the impact of the storage conditions on their biosensing characteristics has not been reported before to our knowledge. To address these issues, we prepared mixed PEO SAMs and tested their stability and biosensing performance in several storage conditions, i.e., air, N2, ethanol, phosphate buffer, and H2O. The quality of the SAMs was monitored as a function of time using various characterization techniques such as cyclic voltammetry, contact angle, grazing angle Fourier-transform infrared spectroscopy, and X-ray photoelectron spectroscopy. In addition, the impact of the different storage conditions on the biosensor properties was investigated using surface plasmon resonance. Via the latter technique, the receptor immobilization, the analyte recognition, and the nonspecific binding were extensively studied using the prostate specific antigen as a model system. Our experiments showed that very small structural differences in the SAM can have a great impact in their final biosensing properties. In addition it was shown that the mixed SAMs stored in air or N2 are very stable and retain their biosensor properties for at least 30 days, while ethanol appeared to be the worst storage medium due to partial oxidation of the thiol headgroup. In conclusion, care must be taken to avoid SAM degradation during storage

  2. Testing new approaches to carbonate system simulation at the reef scale: the ReefSam model first results, application to a question in reef morphology and future challenges.

    NASA Astrophysics Data System (ADS)

    Barrett, Samuel; Webster, Jody

    2016-04-01

    Numerical simulation of the stratigraphy and sedimentology of carbonate systems (carbonate forward stratigraphic modelling - CFSM) provides significant insight into the understanding of both the physical nature of these systems and the processes which control their development. It also provides the opportunity to quantitatively test conceptual models concerning stratigraphy, sedimentology or geomorphology, and allows us to extend our knowledge either spatially (e.g. between bore holes) or temporally (forwards or backwards in time). The later is especially important in determining the likely future development of carbonate systems, particularly regarding the effects of climate change. This application, by its nature, requires successful simulation of carbonate systems on short time scales and at high spatial resolutions. Previous modelling attempts have typically focused on the scales of kilometers and kilo-years or greater (the scale of entire carbonate platforms), rather than at the scale of centuries or decades, and tens to hundreds of meters (the scale of individual reefs). Previous work has identified limitations in common approaches to simulating important reef processes. We present a new CFSM, Reef Sedimentary Accretion Model (ReefSAM), which is designed to test new approaches to simulating reef-scale processes, with the aim of being able to better simulate the past and future development of coral reefs. Four major features have been tested: 1. A simulation of wave based hydrodynamic energy with multiple simultaneous directions and intensities including wave refraction, interaction, and lateral sheltering. 2. Sediment transport simulated as sediment being moved from cell to cell in an iterative fashion until complete deposition. 3. A coral growth model including consideration of local wave energy and composition of the basement substrate (as well as depth). 4. A highly quantitative model testing approach where dozens of output parameters describing the reef

  3. TEACHER-ADVISORS: Where There's a Skill There's A Way.

    ERIC Educational Resources Information Center

    Tamminen, Armas; And Others

    This report discusses a program to present the Teacher Advisement Training Model. This model for training teacher-advisors is based on the assumption that tentative commitment to making school a more rewarding experience for all is the first step in starting an effective program. The approach is to help teachers learn specific skills and methods…

  4. Threat expert system technology advisor

    NASA Technical Reports Server (NTRS)

    Kurrasch, E. R.; Tripp, L. R.

    1987-01-01

    A prototype expert system was developed to determine the feasibility of using expert system technology to enhance the performance and survivability of helicopter pilots in a combat threat environment while flying NOE (Nap of the Earth) missions. The basis for the concept is the potential of using an Expert System Advisor to reduce the extreme overloading of the pilot who flies NOE mission below treetop level at approximately 40 knots while performing several other functions. The ultimate goal is to develop a Threat Expert System Advisor which provides threat information and advice that are better than even a highly experienced copilot. The results clearly show that the NOE pilot needs all the help in decision aiding and threat situation awareness that he can get. It clearly shows that heuristics are important and that an expert system for combat NOE helicopter missions can be of great help to the pilot in complex threat situations and in making decisions.

  5. Omega-3 polyunsaturated fatty acids ameliorate the severity of ileitis in the senescence accelerated mice (SAM)P1/Yit mice model

    PubMed Central

    Matsunaga, H; Hokari, R; Kurihara, C; Okada, Y; Takebayashi, K; Okudaira, K; Watanabe, C; Komoto, S; Nakamura, M; Tsuzuki, Y; Kawaguchi, A; Nagao, S; Miura, S

    2009-01-01

    Clinical studies using omega-3 polyunsaturated fatty acids (ω3-PUFA) to Crohn's disease (CD) are conflicting. Beneficial effects of dietary ω3-PUFA intake in various experimental inflammatory bowel disease (IBD) models have been reported. However, animal models of large intestinal inflammation have been used in all previous studies, and the effect of ω3 fat in an animal model of small intestinal inflammation has not been reported. We hypothesized that the effects of ω3 fat are different between large and small intestine. The aim of this study was to determine whether the direct effect of ω3 fat is beneficial for small intestinal inflammation. Senescence accelerated mice (SAM)P1/Yit mice showed remarkable inflammation of the terminal ileum spontaneously. The numbers of F4/80-positive monocyte–macrophage cells as well as β7-integrin-positive lymphocytes in the intestinal mucosa were increased significantly compared with those in the control mice (AKR-J mice). The area of mucosal addressin cell adhesion molecule-1 (MAdCAM-1)-positive vessels was also increased. The degree of expression levels of monocyte chemoattractant protein-1 (MCP-1), interleukin (IL)-6 and interferon (IFN)-γ mRNA were increased significantly compared with those in the control mice. The feeding of two different kinds of ω3 fat (fish-oil-rich and perilla-oil-rich diets) for 16 weeks to SAMP1/Yit mice ameliorated inflammation of the terminal ileum significantly. In both the ω3-fat-rich diet groups, enhanced infiltration of F4/80-positive monocytes/macrophages in intestinal mucosa of SAMP1/Yit mice cells and the increased levels of MCP-1, IL-6 and IFN-γ mRNA expression were ameliorated significantly compared with those in the control diet group. The results suggest that ω3 fat is beneficial for small intestinal inflammation by inhibition of monocyte recruitment to inflamed intestinal mucosa. PMID:19793338

  6. Full Range Advising: Transforming the Advisor-Advisee Experience

    ERIC Educational Resources Information Center

    Barbuto, John E., Jr.; Story, Joana S.; Fritz, Susan M.; Schinstock, Jack L.

    2011-01-01

    Drawing from the leadership literature, a new model for advising is proposed. Full range advising encompasses laissez-faire, management by exception, contingent rewards, and transformational behaviors. The relationships between full range advising and advisees' extra effort, satisfaction with the advisor, and advising effectiveness were examined.…

  7. Advisor-Advisee Relationships in Graduate Training Programs

    ERIC Educational Resources Information Center

    Schlosser, Lewis Z.; Lyons, Heather Z.; Talleyrand, Regine M.; Kim, Bryan S. K.; Johnson, W. Brad

    2011-01-01

    Advisor-advisee relationships are an important aspect of the career development of professionals in many fields; however, limited scholarship has focused on these relationships. In the three articles of this special section, the authors attempt to help remedy this situation by articulating a culturally infused model of advising relationships in…

  8. SAM 2 data user's guide

    NASA Technical Reports Server (NTRS)

    Chu, W. P.; Osborn, M. T.; Mcmaster, L. R.

    1988-01-01

    This document is intended to serve as a guide to the use of the data products from the Stratospheric Aerosol Measurement (SAM) 2 experiment for scientific investigations of polar stratospheric aerosols. Included is a detailed description of the Beta and Aerosol Number Density Archive Tape (BANAT), which is the SAM 2 data product containing the aerosol extinction data available for these investigations. Also included are brief descriptions of the instrument operation, data collection, processing and validation, and some of the scientific analyses conducted to date.

  9. The Role of Doctoral Advisors: A Look at Advising from the Advisor's Perspective

    ERIC Educational Resources Information Center

    Barnes, Benita J.; Austin, Ann E.

    2009-01-01

    The doctoral advisor is said to be one of the most important persons--if not "the" single most critical person--with whom doctoral students will develop a relationship during their doctoral degree programs (Baird 1995). However, we have limited knowledge regarding how doctoral advisors see their roles and responsibilities as advisors. Therefore,…

  10. Flight deck engine advisor

    NASA Technical Reports Server (NTRS)

    Shontz, W. D.; Records, R. M.; Antonelli, D. R.

    1992-01-01

    The focus of this project is on alerting pilots to impending events in such a way as to provide the additional time required for the crew to make critical decisions concerning non-normal operations. The project addresses pilots' need for support in diagnosis and trend monitoring of faults as they affect decisions that must be made within the context of the current flight. Monitoring and diagnostic modules developed under the NASA Faultfinder program were restructured and enhanced using input data from an engine model and real engine fault data. Fault scenarios were prepared to support knowledge base development activities on the MONITAUR and DRAPhyS modules of Faultfinder. An analysis of the information requirements for fault management was included in each scenario. A conceptual framework was developed for systematic evaluation of the impact of context variables on pilot action alternatives as a function of event/fault combinations.

  11. SOLUTION STRUCTURE OF THE FIRST SAM DOMAIN OF ODIN AND BINDING STUDIES WITH THE EPHA2 RECEPTOR

    PubMed Central

    Mercurio, Flavia Anna; Marasco, Daniela; Pirone, Luciano; Pedone, Emilia Maria; Pellecchia, Maurizio; Leone, Marilisa

    2012-01-01

    The EphA2 receptor plays key roles in many physiological and pathological events including cancer. The process of receptor endocytosis and the consequent degradation have lately attracted attention as possible means of overcoming the negative outcomes of EphA2 in cancer cells and decreasing tumor malignancy. A recent study indicates that Sam (Sterile Alpha Motif) domains of Odin, a member of the ANKS (Ankyrin repeat and sterile alpha motif domain-containing) family of proteins, are important to regulate EphA2 endocytosis. Odin contains two tandem Sam domains (Odin-Sam1 and Sam2). Herein we report on the NMR solution structure of Odin-Sam1; through a variety of assays (employing NMR, SPR and ITC techniques), we clearly demonstrate that Odin-Sam1 binds to the Sam domain of EphA2 in the low micromolar range. NMR chemical shift perturbation experiments and molecular modeling studies point out that the two Sam domains interact with a head to tail topology characteristic of several Sam-Sam complexes. This binding mode is similar to that we have previously proposed for the association between the Sam domains of the lipid phosphatase Ship2 and EphA2. This work further validates structural elements relevant for the heterotypic Sam-Sam interactions of EphA2 and provides novel insights for the design of potential therapeutic compounds that can modulate receptor endocytosis. PMID:22332920

  12. Simulation Test Of Descent Advisor

    NASA Technical Reports Server (NTRS)

    Davis, Thomas J.; Green, Steven M.

    1991-01-01

    Report describes piloted-simulation test of Descent Advisor (DA), subsystem of larger automation system being developed to assist human air-traffic controllers and pilots. Focuses on results of piloted simulation, in which airline crews executed controller-issued descent advisories along standard curved-path arrival routes. Crews able to achieve arrival-time precision of plus or minus 20 seconds at metering fix. Analysis of errors generated in turns resulted in further enhancements of algorithm to increase accuracies of its predicted trajectories. Evaluations by pilots indicate general support for DA concept and provide specific recommendations for improvement.

  13. SAM II Data and Information (ASCII)

    Atmospheric Science Data Center

    2016-09-01

    SAM II (ASCII) Data and Information Data obtained from the Stratospheric Aerosol Measurement (SAM) II instrument, ... Parameters:  Aerosols Order Data:  ASDC Order Tool:  Order Data Guide Documents:  ...

  14. SAM Overview: The Habitability of Mars

    NASA Video Gallery

    Featuring an interview with Paul Mahaffy, SAM's Principal Investigator, this video gives a general overview of SAM's mission aboard the Mars Science Laboratory, otherwise known as the Curiosity rover.

  15. The Vesicle Protein SAM-4 Regulates the Processivity of Synaptic Vesicle Transport

    PubMed Central

    Zheng, Qun; Ahlawat, Shikha; Schaefer, Anneliese; Mahoney, Tim; Koushika, Sandhya P.; Nonet, Michael L.

    2014-01-01

    Axonal transport of synaptic vesicles (SVs) is a KIF1A/UNC-104 mediated process critical for synapse development and maintenance yet little is known of how SV transport is regulated. Using C. elegans as an in vivo model, we identified SAM-4 as a novel conserved vesicular component regulating SV transport. Processivity, but not velocity, of SV transport was reduced in sam-4 mutants. sam-4 displayed strong genetic interactions with mutations in the cargo binding but not the motor domain of unc-104. Gain-of-function mutations in the unc-104 motor domain, identified in this study, suppress the sam-4 defects by increasing processivity of the SV transport. Genetic analyses suggest that SAM-4, SYD-2/liprin-α and the KIF1A/UNC-104 motor function in the same pathway to regulate SV transport. Our data support a model in which the SV protein SAM-4 regulates the processivity of SV transport. PMID:25329901

  16. Faculty Advisors' Experiences with International Graduate Students

    ERIC Educational Resources Information Center

    Nguyen, Huynh Mai

    2013-01-01

    The current study explored the experiences of faculty advisors working with international graduate students (IGS). Data was collected through semi-structured interviews and a demographic survey and was analyzed using open, axial, and selective coding strategies. Three central categories emerged from the analysis: (a) advisors' perceptions of IGS,…

  17. Student and Advisor Attitudes toward Academic Advisement.

    ERIC Educational Resources Information Center

    Wood, Jane H.; Wood, Peter H.

    Three separate but similar questionnaires were mailed to faculty advisors, to secondary education majors, and to nonsecondary education majors in the College of Education and Allied Professions at Bowling Green State University. The majority of each group identified assistance with courses and scheduling at the advisors' most important function.…

  18. SPILC: An expert student advisor

    NASA Technical Reports Server (NTRS)

    Read, D. R.

    1990-01-01

    The Lamar University Computer Science Department serves about 350 undergraduate C.S. majors, and 70 graduate majors. B.S. degrees are offered in Computer Science and Computer and Information Science, and an M.S. degree is offered in Computer Science. In addition, the Computer Science Department plays a strong service role, offering approximately sixteen service course sections per long semester. The department has eight regular full-time faculty members, including the Department Chairman and the Undergraduate Advisor, and from three to seven part-time faculty members. Due to the small number of regular faculty members and the resulting very heavy teaching loads, undergraduate advising has become a difficult problem for the department. There is a one week early registration period and a three-day regular registration period once each semester. The Undergraduate Advisor's regular teaching load of two classes, 6 - 8 semester hours, per semester, together with the large number of majors and small number of regular faculty, cause long queues and short tempers during these advising periods. The situation is aggravated by the fact that entering freshmen are rarely accompanied by adequate documentation containing the facts necessary for proper counselling. There has been no good method of obtaining necessary facts and documenting both the information provided by the student and the resulting advice offered by the counsellors.

  19. Feature-Based Measuring Advisor, Version 2.1

    2002-07-22

    The purpose of Feature-Based Measuring Advisor (FBMeas Advisor) is a graphical user interface enabling users to more effectively interact with a solid model for measuring applications. Specifically, the FBMeas Advisor provides a user interface to the functions and capabilities of the FBMeas component technology. The FBMeas Advisor allows end users to visualize, organize, and document their measuring process plans and to facilitate the generation of a coordinate measuring machine part programs before a workpiece ismore » fabricated. The FBMeas Advisor permits the user to interact with an FBTol-enabled solid model to interactively and/or automatically create a set of measuring features (e.g., cylinders, planes, slots) that comply with national and international interface standards (i.e., DMIS (Dimensional Measuring Interface Standard, ISO 22063)). Measuring plans include defining measuring features interactively or automatically. Associating measuring features with appropriate tolerances and datum features via FBTol. Determining what measuring tasks (e.g., measure this feature to validate a size tolerance, measure this feature to establish a primary datum for a specific datum reference frame) are necessary to measure the feature. Assigning a measuring method to perform the task. For coordinate metrology measuring methods, measure points are determined interactively or automatically. Sensors and sensor configurations are defined, represented, and assigned to measuring methods. Measuring machines are defined, represented, and assigned to measure operations. Upon completion of a coordinate metrology measuring plan, FBMeas allows DMIS code segments to be generated, which can be used for DMIS-based CMM part programs.« less

  20. The nutrition advisor expert system

    NASA Technical Reports Server (NTRS)

    Huse, Scott M.; Shyne, Scott S.

    1991-01-01

    The Nutrition Advisor Expert System (NAES) is an expert system written in the C Language Integrated Production System (CLIPS). NAES provides expert knowledge and guidance into the complex world of nutrition management by capturing the knowledge of an expert and placing it at the user's fingertips. Specifically, NAES enables the user to: (1) obtain precise nutrition information for food items; (2) perform nutritional analysis of meal(s), flagging deficiencies based upon the U.S. Recommended Daily Allowances; (3) predict possible ailments based upon observed nutritional deficiency trends; (4) obtain a top ten listing of food items for a given nutrient; and (5) conveniently upgrade the data base. An explanation facility for the ailment prediction feature is also provided to document the reasoning process.

  1. SAMS Acceleration Measurements on MIR

    NASA Technical Reports Server (NTRS)

    Moskowitz, Milton E.; Hrovat, Kenneth; Finkelstein, Robert; Reckart, Timothy

    1997-01-01

    During NASA Increment 3 (September 1996 to January 1997), about 5 gigabytes of acceleration data were collected by the Space Acceleration Measurement System (SAMS) onboard the Russian Space Station, Mir. The data were recorded on 11 optical disks and were returned to Earth on STS-81. During this time, SAMS data were collected in the Priroda module to support the following experiments: the Mir Structural Dynamics Experiment (MiSDE) and Binary Colloidal Alloy Tests (BCAT). This report points out some of the salient features of the microgravity environment to which these experiments were exposed. Also documented are mission events of interest such as the docked phase of STS-81 operations, a Progress engine burn, attitude control thruster operation, and crew exercise. Also included are a description of the Mir module orientations, and the panel notations within the modules. This report presents an overview of the SAMS acceleration measurements recorded by 10 Hz and 100 Hz sensor heads. Variations in the acceleration environment caused by unique activities such as crew exercise and life-support fans are presented. The analyses included herein complement those presented in previous mission summary reports published by the Principal Investigator Microgravity Services (PIMS) group.

  2. Make your physician advisor your closest ally.

    PubMed

    2016-06-01

    As the pressure increases for case managers to help ensure that hospitals comply with payer requirements and get paid appropriately, physician advisors can be a valuable ally. Physician advisors have clinical expertise as well as credibility with the physician staff, which gives them an advantage when it comes to educating physicians about patient status, documentation needs, patient throughput, and transitions in care. Case managers and their physician advisors should work together to ensure that everything in the patient record is correct up front so the hospital will avoid costly appeals when the claim is denied. Good physician advisors have the kind of personality that enables them to have difficult conversations as well as being well-respected by their colleagues, knowledgeable about admission criteria and reimbursement, accessible, approachable, articulate, and collaborative. PMID:27323506

  3. Air-Traffic Controllers Evaluate The Descent Advisor

    NASA Technical Reports Server (NTRS)

    Tobias, Leonard; Volckers, Uwe; Erzberger, Heinz

    1992-01-01

    Report describes study of Descent Advisor algorithm: software automation aid intended to assist air-traffic controllers in spacing traffic and meeting specified times or arrival. Based partly on mathematical models of weather conditions and performances of aircraft, it generates suggested clearances, including top-of-descent points and speed-profile data to attain objectives. Study focused on operational characteristics with specific attention to how it can be used for prediction, spacing, and metering.

  4. Sam.

    ERIC Educational Resources Information Center

    Wilson, James C.

    1988-01-01

    A father writes about his six-year-old son born with hydrocephalus. He describes such day-to-day experiences as going to a baseball game and the grocery store, reactions of friends and strangers to his son's social interactions, and a special day at preschool. The boy's medical treatment, including surgeries, are also described. (VW)

  5. Sam37 is crucial for formation of the mitochondrial TOM–SAM supercomplex, thereby promoting β-barrel biogenesis

    PubMed Central

    Wenz, Lena-Sophie; Ellenrieder, Lars; Qiu, Jian; Bohnert, Maria; Zufall, Nicole; van der Laan, Martin; Becker, Thomas

    2015-01-01

    Biogenesis of mitochondrial β-barrel proteins requires two preprotein translocases, the general translocase of the outer membrane (TOM) and the sorting and assembly machinery (SAM). TOM and SAM form a supercomplex that promotes transfer of β-barrel precursors. The SAM core complex contains the channel protein Sam50, which cooperates with Sam35 in precursor recognition, and the peripheral membrane protein Sam37. The molecular function of Sam37 has been unknown. We report that Sam37 is crucial for formation of the TOM–SAM supercomplex. Sam37 interacts with the receptor domain of Tom22 on the cytosolic side of the mitochondrial outer membrane and links TOM and SAM complexes. Sam37 thus promotes efficient transfer of β-barrel precursors to the SAM complex. We conclude that Sam37 functions as a coupling factor of the translocase supercomplex of the mitochondrial outer membrane. PMID:26416958

  6. A piloted simulator evaluation of a ground-based 4D descent advisor algorithm

    NASA Technical Reports Server (NTRS)

    Green, Steven M.; Davis, Thomas J.; Erzberger, Heinz

    1987-01-01

    A ground-based, four-dimensional (4D) descent-advisor algorithm is under development at NASA Ames Research Center. The algorithm combines detailed aerodynamic, propulsive, and atmospheric models with an efficient numerical integration scheme to generate 4D descent advisories. This paper investigates the ability of the 4D descent advisor algorithm to provide adequate control of arrival time for aircraft not equipped with on-board 4D guidance systems. A piloted simulation was conducted to determine the precision with which the descent advisor could predict the 4D trajectories of typical straight-in descents flown by airline pilots under different wind conditions. The effects of errors in the estimation of wind and initial aircraft weight were also studied. A description of the descent advisor as well as the results of the simulation studies are presented.

  7. A piloted simulator evaluation of a ground-based 4-D descent advisor algorithm

    NASA Technical Reports Server (NTRS)

    Davis, Thomas J.; Green, Steven M.; Erzberger, Heinz

    1990-01-01

    A ground-based, four dimensional (4D) descent-advisor algorithm is under development at NASA-Ames. The algorithm combines detailed aerodynamic, propulsive, and atmospheric models with an efficient numerical integration scheme to generate 4D descent advisories. The ability is investigated of the 4D descent advisor algorithm to provide adequate control of arrival time for aircraft not equipped with on-board 4D guidance systems. A piloted simulation was conducted to determine the precision with which the descent advisor could predict the 4D trajectories of typical straight-in descents flown by airline pilots under different wind conditions. The effects of errors in the estimation of wind and initial aircraft weight were also studied. A description of the descent advisor as well as the result of the simulation studies are presented.

  8. SAM -- A Spectral Extraction Package

    NASA Astrophysics Data System (ADS)

    Lewis, J. R.

    In this note a description is given of SAM, a package written at RGO for the extraction of spectra from two dimensional data frames. The need to extract spectra from two dimensional frames in an optimal manner (e.g. one in which the signal to noise ratio was maximised) was the primary reason for the writing of the package. The programs were originally written with FOS, ISIS and IDS in mind, but contain nothing which is instrument specific and hence should be applicable to any two dimensional spectral data frame.

  9. Descent Advisor Preliminary Field Test

    NASA Technical Reports Server (NTRS)

    Green, Steven M.; Vivona, Robert A.; Sanford, Beverly

    1995-01-01

    A field test of the Descent Advisor (DA) automation tool was conducted at the Denver Air Route Traffic Control Center in September 1994. DA is being developed to assist Center controllers in the efficient management and control of arrival traffic. DA generates advisories, based on trajectory predictions, to achieve accurate meter-fix arrival times in a fuel efficient manner while assisting the controller with the prediction and resolution of potential conflicts. The test objectives were: (1) to evaluate the accuracy of DA trajectory predictions for conventional and flight-management system equipped jet transports, (2) to identify significant sources of trajectory prediction error, and (3) to investigate procedural and training issues (both air and ground) associated with DA operations. Various commercial aircraft (97 flights total) and a Boeing 737-100 research aircraft participated in the test. Preliminary results from the primary test set of 24 commercial flights indicate a mean DA arrival time prediction error of 2.4 seconds late with a standard deviation of 13.1 seconds. This paper describes the field test and presents preliminary results for the commercial flights.

  10. Descent advisor preliminary field test

    NASA Technical Reports Server (NTRS)

    Green, Steven M.; Vivona, Robert A.; Sanford, Beverly

    1995-01-01

    A field test of the Descent Advisor (DA) automation tool was conducted at the Denver Air Route Traffic Control Center in September 1994. DA is being developed to assist Center controllers in the efficient management and control of arrival traffic. DA generates advisories, based on trajectory predictions, to achieve accurate meter-fix arrival times in a fuel efficient manner while assisting the controller with the prediction and resolution of potential conflicts. The test objectives were to evaluate the accuracy of DA trajectory predictions for conventional- and flight-management-system-equipped jet transports, to identify significant sources of trajectory prediction error, and to investigate procedural and training issues (both air and ground) associated with DA operations. Various commercial aircraft (97 flights total) and a Boeing 737-100 research aircraft participated in the test. Preliminary results from the primary test set of 24 commercial flights indicate a mean DA arrival time prediction error of 2.4 sec late with a standard deviation of 13.1 sec. This paper describes the field test and presents preliminary results for the commercial flights.

  11. Design of a knowledge-based welding advisor

    SciTech Connect

    Kleban, S.D.

    1996-06-01

    Expert system implementation can take numerous forms ranging form traditional declarative rule-based systems with if-then syntax to imperative programming languages that capture expertise in procedural code. The artificial intelligence community generally thinks of expert systems as rules or rule-bases and an inference engine to process the knowledge. The welding advisor developed at Sandia National Laboratories and described in this paper deviates from this by codifying expertise using object representation and methods. Objects allow computer scientists to model the world as humans perceive it giving us a very natural way to encode expert knowledge. The design of the welding advisor, which generates and evaluates solutions, will be compared and contrasted to a traditional rule- based system.

  12. SAM-2 ground-truth plan: Correlative measurements for the Stratospheric Aerosol Measurement-2 (SAM 2) sensor on the Nimbus G satellite

    NASA Technical Reports Server (NTRS)

    Russell, P. B.; Mccormick, M. P.; Mcmaster, L. R.; Pepin, T. J.; Chu, W. P.; Swissler, T. J.

    1978-01-01

    The SAM-2 will fly aboard the Nimbus-G satellite for launch in the fall of 1978 and measure stratospheric vertical profiles of aerosol extinction in high latitude bands. The plan gives details of the location and times for the simultaneous satellite/correlative measurements for the nominal launch time, the rationale and choice of the correlative sensors, their characteristics and expected accuracies, and the conversion of their data to extinction profiles. The SAM-2 expected instrument performance and data inversion results are presented. Various atmospheric models representative of polar stratospheric aerosols are used in the SAM-2 and correlative sensor analyses.

  13. Understanding the Electronic Structure of Metal/SAM/Organic−Semiconductor Heterojunctions

    PubMed Central

    2009-01-01

    Computational modeling is used to describe the mechanisms governing energy level alignment between an organic semiconductor (OSC) and a metal covered by various self-assembled monolayers (SAMs). In particular, we address the question to what extent and under what circumstances SAM-induced work-function modifications lead to an actual change of the barriers for electron and hole injection from the metal into the OSC layer. Depending on the nature of the SAM, we observe clear transitions between Fermi level pinning and vacuum-level alignment regimes. Surprisingly, although in most cases the pinning occurs only when the metal is present, it is not related to charge transfer between the electrode and the organic layer. Instead, charge rearrangements at the interface between the SAM and the OSC are observed, accompanied by a polarization of the SAM. PMID:19891441

  14. Surface Hold Advisor Using Critical Sections

    NASA Technical Reports Server (NTRS)

    Law, Caleb Hoi Kei (Inventor); Hsiao, Thomas Kun-Lung (Inventor); Mittler, Nathan C. (Inventor); Couluris, George J. (Inventor)

    2013-01-01

    The Surface Hold Advisor Using Critical Sections is a system and method for providing hold advisories to surface controllers to prevent gridlock and resolve crossing and merging conflicts among vehicles traversing a vertex-edge graph representing a surface traffic network on an airport surface. The Advisor performs pair-wise comparisons of current position and projected path of each vehicle with other surface vehicles to detect conflicts, determine critical sections, and provide hold advisories to traffic controllers recommending vehicles stop at entry points to protected zones around identified critical sections. A critical section defines a segment of the vertex-edge graph where vehicles are in crossing or merging or opposite direction gridlock contention. The Advisor detects critical sections without reference to scheduled, projected or required times along assigned vehicle paths, and generates hold advisories to prevent conflicts without requiring network path direction-of-movement rules and without requiring rerouting, rescheduling or other network optimization solutions.

  15. Characterization of the SAM domain of the PKD-related protein ANKS6 and its interaction with ANKS3

    PubMed Central

    2014-01-01

    Background Autosomal dominant polycystic kidney disease (ADPKD) is the most common genetic disorder leading to end-stage renal failure in humans. In the PKD/Mhm(cy/+) rat model of ADPKD, the point mutation R823W in the sterile alpha motif (SAM) domain of the protein ANKS6 is responsible for disease. SAM domains are known protein-protein interaction domains, capable of binding each other to form polymers and heterodimers. Despite its physiological importance, little is known about the function of ANKS6 and how the R823W point mutation leads to PKD. Recent work has revealed that ANKS6 interacts with a related protein called ANKS3. Both ANKS6 and ANKS3 have a similar domain structure, with ankyrin repeats at the N-terminus and a SAM domain at the C-terminus. Results The SAM domain of ANKS3 is identified as a direct binding partner of the ANKS6 SAM domain. We find that ANKS3-SAM polymerizes and ANKS6-SAM can bind to one end of the polymer. We present crystal structures of both the ANKS3-SAM polymer and the ANKS3-SAM/ANKS6-SAM complex, revealing the molecular details of their association. We also learn how the R823W mutation disrupts ANKS6 function by dramatically destabilizing the SAM domain such that the interaction with ANKS3-SAM is lost. Conclusions ANKS3 is a direct interacting partner of ANKS6. By structurally and biochemically characterizing the interaction between the ANKS3 and ANKS6 SAM domains, our work provides a basis for future investigation of how the interaction between these proteins mediates kidney function. PMID:24998259

  16. 31 CFR 10.33 - Best practices for tax advisors.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 31 Money and Finance: Treasury 1 2012-07-01 2012-07-01 false Best practices for tax advisors. 10... Service § 10.33 Best practices for tax advisors. (a) Best practices. Tax advisors should provide clients with the highest quality representation concerning Federal tax issues by adhering to best practices...

  17. 31 CFR 10.33 - Best practices for tax advisors.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 31 Money and Finance: Treasury 1 2014-07-01 2014-07-01 false Best practices for tax advisors. 10... Service § 10.33 Best practices for tax advisors. (a) Best practices. Tax advisors should provide clients with the highest quality representation concerning Federal tax issues by adhering to best practices...

  18. 31 CFR 10.33 - Best practices for tax advisors.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 31 Money and Finance: Treasury 1 2011-07-01 2011-07-01 false Best practices for tax advisors. 10... Service § 10.33 Best practices for tax advisors. (a) Best practices. Tax advisors should provide clients with the highest quality representation concerning Federal tax issues by adhering to best practices...

  19. 31 CFR 10.33 - Best practices for tax advisors.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Best practices for tax advisors. 10... Service § 10.33 Best practices for tax advisors. (a) Best practices. Tax advisors should provide clients with the highest quality representation concerning Federal tax issues by adhering to best practices...

  20. 31 CFR 10.33 - Best practices for tax advisors.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 31 Money and Finance: Treasury 1 2013-07-01 2013-07-01 false Best practices for tax advisors. 10... Service § 10.33 Best practices for tax advisors. (a) Best practices. Tax advisors should provide clients with the highest quality representation concerning Federal tax issues by adhering to best practices...

  1. 75 FR 54465 - Temporary Registration of Municipal Advisors

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-08

    ... Commission is adopting an interim final temporary rule that establishes a means for municipal advisors, as... becomes effective on October 1, 2010, meaning that municipal advisors must be registered on that date in... Section 975(e) of the Dodd-Frank Act, defines the term ``municipal advisor'' to mean a person (who is...

  2. NES Live Video Chat: Engineer Sam Ortega

    NASA Video Gallery

    The NES project invited all K-12 students to participate in a one-hour-long NASA video webchat on April 19, 2011 with NASA engineer Sam Ortega. Ortega answered questions about building and testing ...

  3. STS-134 Crew Talks With Sam Ting

    NASA Video Gallery

    The STS-134 crew talks with Sam Ting, principal investigator for the Alpha Magnetic Spectrometer, following the installation of the particle physics detector on the International Space Station duri...

  4. Stay lean without dieting: Lose Sam68.

    PubMed

    Huot, Marc-Étienne; Richard, Stéphane

    2012-10-01

    Alternative splicing is well known to be tissue-specific. Although several genes have been shown to undergo alternative splicing in adipocytes, little is known about the mechanism that regulates alternative splicing during adipogenesis. We recently reported that Sam68(-/-) mice exhibit a lean phenotype and are protected against diet-induced obesity. Our genome-wide exon array analysis in white adipose tissue (WAT) from wild-type and Sam68(-/-) mice revealed that Sam68 deficiency leads to an abnormal splicing of the mTOR gene. This has been shown to reduce the overall mTOR protein content and activity during in vitro adipose differentiation. In Sam68(-/-) mice, this situation leads to an increased energy expenditure, decreased adipogenesis and WAT formation. PMID:23700540

  5. Sam Donaldson: Tips from A Cancer Survivor

    MedlinePlus

    ... little bit about the disease now and the survivability of various forms and stages. Klose: Do you ... More "Understanding Cancer" Articles Understanding Cancer / Cancer Today / Survivability and Hope / Sam Donaldson: Tips From a Cancer ...

  6. Solar Effective Envelope Design Advisor (SEEDA)

    NASA Astrophysics Data System (ADS)

    Mahaek, Ekkachai

    The lack of effort by mainstream architects in integrating energy-efficient strategies in architectural designing is due to the complexity in a building's energy conscious concepts and theories, the difficulties to visualize and quantify energy consumption, and the late implementing of energy consumption analysis in the conventional design process. This task would be accomplishing by a building system's engineer where results might be determined only after the basic architectural design has been completed. An effective simple tool and method should then be available to assist architects in building's energy-efficient designing at the beginning of the design. The building's energy consumption is directly and mainly influenced by the relationship of the sun, site, and its building configuration. The solar radiations will first impact on the building's envelope, which will have a direct effect on the amount of energy a building will consume. If an architect can define or map the intensity of solar energy on the site's buildable volume, and use this information to determine the levels of solar insolation, a more energy efficient building form can be proposed. This research hypothesis has shared the fundamental techniques of the Solar Envelope projection by Professor Ralph Knowles [Knowles, 1981] of the University of Southern California. However a different approach is taken by including the influence of regional restrictions and the surrounding buildings' shadows when projecting of solar volumes and solar envelope. The research methodology will discuss the development of a computer-based approach to develop a three-dimensional architectural form based on an insolation map related to the design site. The prototype computer program is referred as the Solar Effective Envelope Design Advisor (SEEDA). The solar insolation volume of the site is determined by integrating three types of computer-generated models include the Buildable Volume model based on design constraints

  7. Modelo Pedagogico de Educacion Primaria para Adultos: Manual para el Asesor de Estudiantes Libres. Primera Parte (A Pedagogical Model for Adult Primary Education: Manual for the Student Advisor. Part One).

    ERIC Educational Resources Information Center

    Instituto Nacional para la Educacion de los Adultos, Mexico City (Mexico).

    This guide, part of a Mexican series of instructional materials, is intended for advisors of students participating in an adult education program offered through public and private organizations in communities in Mexico. The first part of the program comprises Spanish and math; the second, education for family life, education for community life,…

  8. Sam68 modulates apoptosis of intestinal epithelial cells via mediating NF-κB activation in ulcerative colitis.

    PubMed

    Qian, Ji; Zhao, Weijuan; Miao, Xianjing; Li, Liren; Zhang, Dongmei

    2016-07-01

    Sam68 (Src-associated substrate during mitosis of 68 KDa), also known as KHDRBS1 (KH domain containing, RNA binding, signal transduction associated 1), belongs to the prototypic member of the signal transduction activator of RNA (STAR) family of RNA-binding proteins. Sam68 is implicated in various cellular processes including RNA metabolism, apoptosis, signal transduction. Previous researches demonstrated that Sam68 regulated nuclear transcription factor kappa B (NF-κB) to induce inflammation. However, the expression and biological functions of Sam68 in ulcerative colitis (UC) are not clear. In this study, we reported for the first time that Sam68 was up-regulated in intestinal epithelial cells (IECs) of patients with UC. In DSS-induced mouse colitis model, we observed the overexpression of Sam68 accompanied with increased levels of IEC apoptotic markers (active caspase-3 and cleaved PARP) and NF-κB activation indicators (p-p65 and p-IκB) in colitis IECs. Co-localization of Sam68 with active caspase-3 (and p-p65) in IECs of the DSS-induced colitis group further indicated the possible involvement of NF-κB-mediated IEC apoptosis. Applying TNF-α-treated HT-29 cells as an in vitro IEC inflammation model, we confirmed the positive correlation amomg Sam68, NF-κB activation and caspase-dependent apoptosis. Immunofluorescence and immunoprecipitation assay identified nuclear translocation and physical interaction of Sam68 and NF-κB subunits in TNF-α-treated HT-29 cells. Besides, depletion of Sam68 by RNA interference greatly alleviated NF-κB activation and apoptosis in TNF-α-treated HT-29 cells. Taken together, our results indicated that Sam68 modulates apoptosis of intestinal epithelial cells via mediating NF-κB activation in UC. PMID:27235792

  9. Surface adhesion and confinement variation of Bacillus subtilis on SAM surfaces

    NASA Astrophysics Data System (ADS)

    Swiger, Lauren; Pasquale, Rose; Calabrese, Joseph; Senevirathne, Indrajith

    2012-02-01

    Controlled surface adhesion of non - pathogenic gram positive strain, Bacillus subtilis is interesting as a model system due to possible development of respective biosensors for prevention and detection of the pathogenic variants B. anthracis and B. cereus. Further as a study for bio-machine interfacing systems. Self Assembled Monolayers (SAM) with engineered surfaces of linear thiols on Au(111) were used as the substrate. Sub cultured B. subtilis were used for the analysis. The SAM layered surfaces were dipped in 2 -- 5 Log/ml B. subtilis solution. Subsequent surface adhesion at different bacterial dilutions on surfaces will be discussed, and correlated with quantitative and qualitative adhesion properties of bacteria on the engineered SAM surfaces. The bacteria adhered SAM surfaces were investigated using intermittent contact, noncontact, lateral force and contact modes of Atomic Force Microscopy (AFM).

  10. Surface adhesion and confinement variation of Staphylococcus aurius on SAM surfaces

    NASA Astrophysics Data System (ADS)

    Amroski, Alicia; Olsen, Morgan; Calabrese, Joseph; Senevirathne, Reshani; Senevirathne, Indrajith

    2012-02-01

    Controlled surface adhesion of non - pathogenic gram positive strain, Staphylococcus aureus is interesting as a model system due to possible development of respective biosensors for prevention and detection of the pathogenic strain methicillin resistant Staphylococcus aureus (MRSA) and further as a study for bio-machine interfacing. Self Assembled Monolayers (SAM) with engineered surfaces of linear thiols on Au(111) were used as the substrate. Sub cultured S. aureus were used for the analysis. The SAM layered surfaces were dipped in 2 -- 4 Log/ml S. aureus solution. Subsequent surface adhesion at different bacterial dilutions on surfaces will be discussed, and correlated with quantitative and qualitative adhesion properties of bacteria on the engineered SAM surfaces. The bacteria adhered SAM surfaces were investigated using intermittent contact, noncontact, lateral force and contact modes of Atomic Force Microscopy (AFM).

  11. The ALICE Glance Shift Accounting Management System (SAMS)

    NASA Astrophysics Data System (ADS)

    Martins Silva, H.; Abreu Da Silva, I.; Ronchetti, F.; Telesca, A.; Maidantchik, C.

    2015-12-01

    ALICE (A Large Ion Collider Experiment) is an experiment at the CERN LHC (Large Hadron Collider) studying the physics of strongly interacting matter and the quark-gluon plasma. The experiment operation requires a 24 hours a day and 7 days a week shift crew at the experimental site, composed by the ALICE collaboration members. Shift duties are calculated for each institute according to their correlated members. In order to ensure the full coverage of the experiment operation as well as its good quality, the ALICE Shift Accounting Management System (SAMS) is used to manage the shift bookings as well as the needed training. ALICE SAMS is the result of a joint effort between the Federal University of Rio de Janeiro (UFRJ) and the ALICE Collaboration. The Glance technology, developed by the UFRJ and the ATLAS experiment, sits at the basis of the system as an intermediate layer isolating the particularities of the databases. In this paper, we describe the ALICE SAMS development process and functionalities. The database has been modelled according to the collaboration needs and is fully integrated with the ALICE Collaboration repository to access members information and respectively roles and activities. Run, period and training coordinators can manage their subsystem operation and ensure an efficient personnel management. Members of the ALICE collaboration can book shifts and on-call according to pre-defined rights. ALICE SAMS features a user profile containing all the statistics and user contact information as well as the Institutes profile. Both the user and institute profiles are public (within the scope of the collaboration) and show the credit balance in real time. A shift calendar allows the Run Coordinator to plan data taking periods in terms of which subsystems shifts are enabled or disabled and on-call responsible people and slots. An overview display presents the shift crew present in the control room and allows the Run Coordination team to confirm the presence

  12. Genetic characterization of senescence-accelerated mouse (SAM).

    PubMed

    Higuchi, K

    1997-01-01

    The Senescence-Accelerated Mouse (SAM) strains are unique and appropriate models for genetic studies on aging because the SAMP strains have an "accelerated senescence" phenotype for which the SAMR strains are controls, and each SAMP strain has a strain-specific age-associated disorder. Furthermore, because they have gone through sufficient generations of sister-brother mating, they can be considered inbred strains, which can be analyzed genetically. There are now 11 SAMP strains and 3 SAMR strains descended from the progenitor litters. Analysis with the Gompertz function shows that the SAMP strains have the same initial mortality rate (IMR) as the SAMR strains but a shorter mortality rate doubling time (MRDT), presumably due to genes that accelerated the rate of senescence in the SAMP strains. This accelerated senescence may also occur in cultured fibroblast-like cells. We performed molecular genetic characterization of all the SAM strains to acquire a base of genetic information from which we could develop hypotheses on the mechanism of development of SAM strains and genetic factors that contribute to accelerated senescence. PMID:9088910

  13. Sam, Brookhaven, and the Physical Review

    NASA Astrophysics Data System (ADS)

    Blume, Martin

    2010-03-01

    Sam Goudsmit came to Brookhaven National Laboratory in 1948, just after the first year of operation of the new institution, and after a year of his postwar appointment as Professor of Physics at Northwestern University. He was named an associate editor of the Physical Review at that time, under the then Managing Editor John T. Tate of the University of Minnesota. Tate had been Editor since 1926, and had presided over the growth of Physical Review to leadership of publication in the world of physics. Tate died in 1950, and after a search under an interim Editor Sam was, in 1951, named Managing Editor. In 1952 he became Chair of the Brookhaven Physics Department, founded Physical Review Letters, and served as department chair until 1960, when he stepped down but remained an Associate Chair. I will discuss my own interactions with Sam during this later period, when I learned of his many faceted talents and accomplishments.

  14. Validation of SAM 2 and SAGE satellite

    NASA Technical Reports Server (NTRS)

    Kent, G. S.; Wang, P.-H.; Farrukh, U. O.; Yue, G. K.

    1987-01-01

    Presented are the results of a validation study of data obtained by the Stratospheric Aerosol and Gas Experiment I (SAGE I) and Stratospheric Aerosol Measurement II (SAM II) satellite experiments. The study includes the entire SAGE I data set (February 1979 - November 1981) and the first four and one-half years of SAM II data (October 1978 - February 1983). These data sets have been validated by their use in the analysis of dynamical, physical and chemical processes in the stratosphere. They have been compared with other existing data sets and the SAGE I and SAM II data sets intercompared where possible. The study has shown the data to be of great value in the study of the climatological behavior of stratospheric aerosols and ozone. Several scientific publications and user-oriented data summaries have appeared as a result of the work carried out under this contract.

  15. Refined NMR structure of a heterodimeric SAM:SAM complex. Characterization and manipulation of the EhpA2 interface leads to discovery of new cellular functions of SHIP2

    PubMed Central

    Lee, H. J.; Hota, P. K.; Chugha, P.; Guo, H.; Miao, H.; Zhang, L.; Kim, S. J.; Stetzig, L.

    2012-01-01

    The sterile alpha motif (SAM) for protein-protein interactions is encountered in over 200 proteins, but the structural bases for its interactions is just becoming clear. Here we solved the structure of the EphA2-SHIP2 SAM:SAM heterodimeric complex by use of NMR restraints from chemical shift perturbations, NOE and RDC experiments. Specific contacts between the protein surfaces differ significantly from a previous model and from other SAM:SAM complexes. Molecular dynamics and docking simulations indicate fluctuations in the complex towards alternate, higher energy conformations. The interface suggests that EphA family members bind to SHIP2 SAM whereas EphB members may not; correspondingly we demonstrate binding of EphA1 but not of EphB2 to SHIP2 SAM. A variant of EphB2 SAM was designed that binds SHIP2. Functional characterization of a mutant EphA2 compromised in SHIP2 binding reveals two previously unrecognized functions of SHIP2 in suppressing ligand-induced activation of EphA2 and in promoting chemotactic cell migration in coordination with the receptor. PMID:22244754

  16. Demographic Trends and Advocacy Experiences of Gay-Straight Alliance Advisors

    ERIC Educational Resources Information Center

    Graybill, Emily C.; Varjas, Kris; Meyers, Joel; Dever, Bridget V.; Greenberg, Daphne; Roach, Andrew T.; Morillas, Catalina

    2015-01-01

    Using an ecological model, the individual-, school-, and sociocultural-level characteristics that affect gay-straight alliance (GSA) advisors were examined in the current study. The formation of GSAs has been one way that schools have sought to improve the school climate for lesbian, gay, bisexual, and transgender (LGBT) youth. Limited information…

  17. STORED GRAIN ADVISOR PRO: DECISION SUPPORT SYSTEM FOR INSECT MANAGEMENT IN COMMERCIAL GRAIN ELEVATORS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A decision support system, Stored Grain Advisor Pro (SGA Pro), was developed to provide insect pest management information for grain stored at commercial elevators. The program uses a model to predict future risk based on current insect density, grain temperature and moisture. A rule-based system wa...

  18. Empowering Hispanic Students through Student Organizations: Competencies for Faculty Advisors.

    ERIC Educational Resources Information Center

    Delgado-Romero, Edward A.; Hernandez, Carlos A.

    2002-01-01

    Presents preliminary competencies for faculty advisors of Hispanic student organizations; the competencies are presented, defined, and illustrated with examples. Concludes with implications for research. (EV)

  19. Genetic typing of the senescence-accelerated mouse (SAM) strains with microsatellite markers.

    PubMed

    Xia, C; Higuchi, K; Shimizu, M; Matsushita, T; Kogishi, K; Wang, J; Chiba, T; Festing, M F; Hosokawa, M

    1999-03-01

    The Senescence-Accelerated Mouse (SAM) strains constitute a murine model of accelerated senescence originating from the ancestral AKR/J strains and consist of nine senescence-prone (SAMP) strains and four senescence-resistant (SAMR) strains. The chromosomes (Chrs) of the SAM strains were typed with 581 microsatellite markers amplified by PCR, and the fundamental genetic information of the SAM strains was obtained. One-third of the examined markers displayed polymorphism among the strains, and only two alleles were detected in almost all loci among the SAM and AKR/J strains. However, in 12 loci (5.6% of total 215 polymorphic markers), the third allele was detected among the SAM strains. The genetic typing and developmental history suggested that the SAM strains were related inbred strains developed by the accidental crossing between the AKR/J strain and other unknown strain(s). Comparison of the distribution of the loci in the SAMP and the SAMR series revealed notable differences in the four regions on Chrs 4, 14, 16, and 17. This indicated that some of these chromosomal sites might contain the genes responsible for accelerated senescence in the SAMP series. PMID:10051317

  20. Discrimination between closely related cellular metabolites by the SAM-I riboswitch.

    PubMed

    Montange, Rebecca K; Mondragón, Estefanía; van Tyne, Daria; Garst, Andrew D; Ceres, Pablo; Batey, Robert T

    2010-02-26

    The SAM-I riboswitch is a cis-acting element of genetic control found in bacterial mRNAs that specifically binds S-adenosylmethionine (SAM). We previously determined the 2.9-A X-ray crystal structure of the effector-binding domain of this RNA element, revealing details of RNA-ligand recognition. To improve this structure, variations were made to the RNA sequence to alter lattice contacts, resulting in a 0.5-A improvement in crystallographic resolution and allowing for a more accurate refinement of the crystallographic model. The basis for SAM specificity was addressed by a structural analysis of the RNA complexed to S-adenosylhomocysteine (SAH) and sinefungin and by measuring the affinity of SAM and SAH for a series of mutants using isothermal titration calorimetry. These data illustrate the importance of two universally conserved base pairs in the RNA that form electrostatic interactions with the positively charged sulfonium group of SAM, thereby providing a basis for discrimination between SAM and SAH. PMID:20006621

  1. Discrimination between Closely Related Cellular Metabolites by the SAM-I Riboswitch

    SciTech Connect

    Montange, R.; Mondragon, E; van Tyne, D; Garst, A; Ceres, P; Batey, R

    2010-01-01

    The SAM-I riboswitch is a cis-acting element of genetic control found in bacterial mRNAs that specifically binds S-adenosylmethionine (SAM). We previously determined the 2.9-{angstrom} X-ray crystal structure of the effector-binding domain of this RNA element, revealing details of RNA-ligand recognition. To improve this structure, variations were made to the RNA sequence to alter lattice contacts, resulting in a 0.5-{angstrom} improvement in crystallographic resolution and allowing for a more accurate refinement of the crystallographic model. The basis for SAM specificity was addressed by a structural analysis of the RNA complexed to S-adenosylhomocysteine (SAH) and sinefungin and by measuring the affinity of SAM and SAH for a series of mutants using isothermal titration calorimetry. These data illustrate the importance of two universally conserved base pairs in the RNA that form electrostatic interactions with the positively charged sulfonium group of SAM, thereby providing a basis for discrimination between SAM and SAH.

  2. Discrimination between Closely Related Cellular Metabolites by the SAM-I Riboswitch

    PubMed Central

    Montange, Rebecca K.; Mondragón, Estefanía; van Tyne, Daria; Garst, Andrew D.; Ceres, Pablo; Batey, Robert T.

    2009-01-01

    The SAM-I riboswitch is a cis-acting element of genetic control found in bacterial mRNAs that specifically binds S-adenosylmethionine (SAM). We previously determined the 2.9-Å X-ray crystal structure of the effector-binding domain of this RNA element, revealing details of RNA–ligand recognition. To improve this structure, variations were made to the RNA sequence to alter lattice contacts, resulting in a 0.5-Å improvement in crystallographic resolution and allowing for a more accurate refinement of the crystallographic model. The basis for SAM specificity was addressed by a structural analysis of the RNA complexed to S-adenosylhomocysteine (SAH) and sinefungin and by measuring the affinity of SAM and SAH for a series of mutants using isothermal titration calorimetry. These data illustrate the importance of two universally conserved base pairs in the RNA that form electrostatic interactions with the positively charged sulfonium group of SAM, thereby providing a basis for discrimination between SAM and SAH. PMID:20006621

  3. Structural basis for diversity in the SAM clan of riboswitches

    PubMed Central

    Trausch, Jeremiah J.; Xu, Zhenjiang; Edwards, Andrea L.; Reyes, Francis E.; Ross, Phillip E.; Knight, Rob; Batey, Robert T.

    2014-01-01

    In bacteria, sulfur metabolism is regulated in part by seven known families of riboswitches that bind S-adenosyl-l-methionine (SAM). Direct binding of SAM to these mRNA regulatory elements governs a downstream secondary structural switch that communicates with the transcriptional and/or translational expression machinery. The most widely distributed SAM-binding riboswitches belong to the SAM clan, comprising three families that share a common SAM-binding core but differ radically in their peripheral architecture. Although the structure of the SAM-I member of this clan has been extensively studied, how the alternative peripheral architecture of the other families supports the common SAM-binding core remains unknown. We have therefore solved the X-ray structure of a member of the SAM-I/IV family containing the alternative “PK-2” subdomain shared with the SAM-IV family. This structure reveals that this subdomain forms extensive interactions with the helix housing the SAM-binding pocket, including a highly unusual mode of helix packing in which two helices pack in a perpendicular fashion. Biochemical and genetic analysis of this RNA reveals that SAM binding induces many of these interactions, including stabilization of a pseudoknot that is part of the regulatory switch. Despite strong structural similarity between the cores of SAM-I and SAM-I/IV members, a phylogenetic analysis of sequences does not indicate that they derive from a common ancestor. PMID:24753586

  4. More than a Signature: How Advisor Choice and Advisor Behaviour Affect Doctoral Student Satisfaction

    ERIC Educational Resources Information Center

    Zhao, Chun-Mei; Golde, Chris M.; McCormick, Alexander C.

    2007-01-01

    A satisfactory relationship between doctoral students and their advisors is an essential component of successful doctoral training. Using responses to a national survey of doctoral students in the US from 27 universities and 11 disciplines, this paper explores factors affecting students' satisfaction with the advising relationship. We find that…

  5. 78 FR 11702 - AdvisorShares Investments, LLC and AdvisorShares Trust; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-19

    ... reasons discussed below. 6. Applicants assert that the shareholders expect the Advisor, subject to the... subadvisory agreements without shareholder approval and would grant relief from certain disclosure... ``Independent Trustees''), and by the initial shareholder of each Fund in the manner required by sections...

  6. Structural Basis for Methyl Transfer by a Radical SAM Enzyme

    SciTech Connect

    Boal, Amie K.; Grove, Tyler L.; McLaughlin, Monica I.; Yennawar, Neela H.; Booker, Squire J.; Rosenzweig, Amy C.

    2014-10-02

    The radical S-adenosyl-l-methionine (SAM) enzymes RlmN and Cfr methylate 23S ribosomal RNA, modifying the C2 or C8 position of adenosine 2503. The methyl groups are installed by a two-step sequence involving initial methylation of a conserved Cys residue (RlmN Cys{sup 355}) by SAM. Methyl transfer to the substrate requires reductive cleavage of a second equivalent of SAM. Crystal structures of RlmN and RlmN with SAM show that a single molecule of SAM coordinates the [4Fe-4S] cluster. Residue Cys{sup 355} is S-methylated and located proximal to the SAM methyl group, suggesting the SAM that is involved in the initial methyl transfer binds at the same site. Thus, RlmN accomplishes its complex reaction with structural economy, harnessing the two most important reactivities of SAM within a single site.

  7. 77 FR 50493 - Sam Rayburn Dam Project Power Rate

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-21

    ... Southwestern Power Administration Sam Rayburn Dam Project Power Rate AGENCY: Southwestern Power Administration, DOE. ACTION: Notice of proposed extension. SUMMARY: The current Sam Rayburn Dam Project rate was... cost recovery criteria. In accordance with Southwestern's isolated project rate adjustment...

  8. Sam's Journey to "Reach for the Stars"

    ERIC Educational Resources Information Center

    Mayer, Sue

    2007-01-01

    In this article, the author shares her experiences as a parent of a child with Down syndrome. Although her son Sam's first years were filled with numerous hospitalizations and visits to pediatricians, which she feared would further delay his development, she soon discovered an organization known as the National Association of Child Development…

  9. 78 FR 62627 - Sam Rayburn Dam Rate

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-22

    ...The U.S. Department of Energy Deputy Secretary has approved and placed into effect on an interim basis Rate Order No. SWPA-67, which increases the power rate for the Sam Rayburn Dam (Rayburn) project pursuant to the Rayburn rate schedule (SRD-13) to supersede the existing rate...

  10. Emerging themes in radical SAM chemistry

    PubMed Central

    Shisler, Krista A; Broderick, Joan B

    2014-01-01

    Enzymes in the radical SAM (RS) superfamily catalyze a wide variety of reactions through unique radical chemistry. The characteristic markers of the superfamily include a [4Fe–4S] cluster coordinated to the protein via a cysteine triad motif, typically CX3CX2C, with the fourth iron coordinated by S-adenosylmethionine (SAM). The SAM serves as a precursor for a 5′-deoxyadenosyl radical, the central intermediate in nearly all RS enzymes studied to date. The SAM-bound [4Fe–4S] cluster is located within a partial or full triosephosphate isomerase (TIM) barrel where the radical chemistry occurs protected from the surroundings. In addition to the TIM barrel and a RS [4Fe–4S] cluster, many members of the superfamily contain additional domains and/or additional Fe–S clusters. Recently characterized superfamily members are providing new examples of the remarkable range of reactions that can be catalyzed, as well as new structural and mechanistic insights into these fascinating reactions. PMID:23141873

  11. EFFECTS OF CYANOPHAGE SAM-1 UPON 'MICROCYSTIS AERUGINOSA'

    EPA Science Inventory

    Cyanophage SAM-1, which infects Synechoccus cedrorum, Anacystis nidulans and certain strains of Microcystis aeruginosa has been isolated from sewage. The host range of cyanophage SAM-1 differs from those of other reported cyanophages. Phage SAM-1 stocks are rapidly inactivated at...

  12. Expanding Radical SAM Chemistry by Using Radical Addition Reactions and SAM Analogues.

    PubMed

    Ji, Xinjian; Li, Yongzhen; Xie, Liqi; Lu, Haojie; Ding, Wei; Zhang, Qi

    2016-09-19

    Radical S-adenosyl-l-methionine (SAM) enzymes utilize a [4Fe-4S] cluster to bind SAM and reductively cleave its carbon-sulfur bond to produce a highly reactive 5'-deoxyadenosyl (dAdo) radical. In almost all cases, the dAdo radical abstracts a hydrogen atom from the substrates or from enzymes, thereby initiating a highly diverse array of reactions. Herein, we report a change of the dAdo radical-based chemistry from hydrogen abstraction to radical addition in the reaction of the radical SAM enzyme NosL. This change was achieved by using a substrate analogue containing an olefin moiety. We also showed that two SAM analogues containing different nucleoside functionalities initiate the radical-based reactions with high efficiencies. The radical adduct with the olefin produced in the reaction was found to undergo two divergent reactions, and the mechanistic insights into this process were investigated in detail. Our study demonstrates a promising strategy in expanding radical SAM chemistry, providing an effective way to access nucleoside-containing compounds by using radical SAM-dependent reactions. PMID:27573794

  13. Academic Advisors' Learning Styles: Establishing a Baseline and Examining Implications

    ERIC Educational Resources Information Center

    Uhlik, Kim S.; Jones, Pamela E.

    2008-01-01

    The influence of students' learning styles has been increasingly recognized as an integral component of effective higher education; therefore, application of learning styles to academic advising is equally relevant. As academic advisors address student learning styles in the hope of promoting greater student success, the contribution of advisors'…

  14. Five Possible Work Profiles for Full-Time Academic Advisors

    ERIC Educational Resources Information Center

    Steele, George

    2006-01-01

    Five potential work profiles for full-time academic advisors, based on the impact of technology, are proposed. The forces accelerating the impact of technology are identified, and the impact of emerging technologies on full-time advisor practice is discussed.

  15. 78 FR 27444 - Forum Investment Advisors, LLC, et al.;

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-10

    ... COMMISSION Forum Investment Advisors, LLC, et al.; Notice of Application May 6, 2013. AGENCY: Securities and... exemption from sections 12(d)(1)(A) and 12(d)(1)(B) of the Act. Applicants: Forum Investment Advisors, LLC (``FIA''), Forum ETF Trust (the ``Trust''), and Foreside Fund Services, LLC (collectively,...

  16. Microskills: Advisor Behaviors that Improve Communication with Advisees

    ERIC Educational Resources Information Center

    Barnett, Susan; Roach, Scott; Smith, Martha

    2006-01-01

    In writing this article, we wanted to provide advisors practical means with which to optimize their relationships with advisees. First, we describe behavioral micro-skills that can be used by advisors to enhance advising sessions. Attending behaviors (eye contact vocal cadence and tone, verbal tracking, body language) and listening skills (asking…

  17. Academic Advisors of Military and Student Veterans: An Ethnographic Study

    ERIC Educational Resources Information Center

    Miller, Michelle A.

    2015-01-01

    With the introduction of the Post-9/11 GI Bill, there is an influx of active-duty military and student veterans enrolling in postsecondary and graduate-level education. The role of an academic advisor increases significantly with this influx of enrollment. The purpose of this study was to determine how a graduate-level academic advisor perceives…

  18. A study on optimization of hybrid drive train using Advanced Vehicle Simulator (ADVISOR)

    NASA Astrophysics Data System (ADS)

    Same, Adam; Stipe, Alex; Grossman, David; Park, Jae Wan

    This study investigates the advantages and disadvantages of three hybrid drive train configurations: series, parallel, and "through-the-ground" parallel. Power flow simulations are conducted with the MATLAB/Simulink-based software ADVISOR. These simulations are then applied in an application for the UC Davis SAE Formula Hybrid vehicle. ADVISOR performs simulation calculations for vehicle position using a combined backward/forward method. These simulations are used to study how efficiency and agility are affected by the motor, fuel converter, and hybrid configuration. Three different vehicle models are developed to optimize the drive train of a vehicle for three stages of the SAE Formula Hybrid competition: autocross, endurance, and acceleration. Input cycles are created based on rough estimates of track geometry. The output from these ADVISOR simulations is a series of plots of velocity profile and energy storage State of Charge that provide a good estimate of how the Formula Hybrid vehicle will perform on the given course. The most noticeable discrepancy between the input cycle and the actual velocity profile of the vehicle occurs during deceleration. A weighted ranking system is developed to organize the simulation results and to determine the best drive train configuration for the Formula Hybrid vehicle. Results show that the through-the-ground parallel configuration with front-mounted motors achieves an optimal balance of efficiency, simplicity, and cost. ADVISOR is proven to be a useful tool for vehicle power train design for the SAE Formula Hybrid competition. This vehicle model based on ADVISOR simulation is applicable to various studies concerning performance and efficiency of hybrid drive trains.

  19. Advisees' Expectations for Support as Moderator between Advisor Behavior and Advisee Perceptions of Advisor Behavior

    ERIC Educational Resources Information Center

    Fullick, Julia M.; Smith-Jentsch, Kimberly A.

    2013-01-01

    We tested relationships between students' expectations of psychosocial and career support through a peer advising program, the frequency of advisor behaviors consistent with these types of support (coded from transcripts), and advisee perceptions after receiving such support. Participants were 179 advisor-advisee dyads at a large southeastern…

  20. Are We in This Together? Partnerships between Academic Advisors and Advising Administrators and Their Relation to Advisor Job Satisfaction

    ERIC Educational Resources Information Center

    Chabinak, Sarah Elizabeth

    2010-01-01

    Scope and Method of Study: This study examined the relationship between professional academic advisors' job satisfaction and perceptions of respect by administration, involvement in decision making, and autonomy. 290 professional academic advisors from 41 public FT4/MS/HTI Carnegie classified institutions participated in the quantitative study…

  1. Hydrogen transfer in SAM-mediated enzymatic radical reactions.

    PubMed

    Hioe, Johnny; Zipse, Hendrik

    2012-12-14

    S-adenosylmethionine (SAM) plays an essential role in a variety of enzyme-mediated radical reactions. One-electron reduction of SAM is currently believed to generate the C5'-desoxyadenosyl radical, which subsequently abstracts a hydrogen atom from the actual substrate in a catalytic or a non-catalytic fashion. Using a combination of theoretical and experimental bond dissociation energy (BDE) data, the energetics of these radical processes have now been quantified. SAM-derived radicals are found to react with their respective substrates in an exothermic fashion in enzymes using SAM in a stoichiometric (non-catalytic) way. In contrast, the catalytic use of SAM appears to be linked to a sequence of moderately endothermic and exothermic reaction steps. The use of SAM in spore photoproduct lyase (SPL) appears to fit neither of these general categories and appears to constitute the first example of a SAM-initiated radical reaction propagated independently of the cofactor. PMID:23139189

  2. Mitochondrial Sorting and Assembly Machinery Subunit Sam37 in Candida albicans: Insight into the Roles of Mitochondria in Fitness, Cell Wall Integrity, and Virulence

    PubMed Central

    Qu, Yue; Jelicic, Branka; Pettolino, Filomena; Perry, Andrew; Lo, Tricia L.; Hewitt, Victoria L.; Bantun, Farkad; Beilharz, Traude H.; Peleg, Anton Y.; Lithgow, Trevor; Djordjevic, Julianne T.

    2012-01-01

    Recent studies indicate that mitochondrial functions impinge on cell wall integrity, drug tolerance, and virulence of human fungal pathogens. However, the mechanistic aspects of these processes are poorly understood. We focused on the mitochondrial outer membrane SAM (Sorting and Assembly Machinery) complex subunit Sam37 in Candida albicans. Inactivation of SAM37 in C. albicans leads to a large reduction in fitness, a phenotype not conserved with the model yeast Saccharomyces cerevisiae. Our data indicate that slow growth of the sam37ΔΔ mutant results from mitochondrial DNA loss, a new function for Sam37 in C. albicans, and from reduced activity of the essential SAM complex subunit Sam35. The sam37ΔΔ mutant was hypersensitive to drugs that target the cell wall and displayed altered cell wall structure, supporting a role for Sam37 in cell wall integrity in C. albicans. The sensitivity of the mutant to membrane-targeting antifungals was not significantly altered. The sam37ΔΔ mutant was avirulent in the mouse model, and bioinformatics showed that the fungal Sam37 proteins are distant from their animal counterparts and could thus represent potential drug targets. Our study provides the first direct evidence for a link between mitochondrial function and cell wall integrity in C. albicans and is further relevant for understanding mitochondrial function in fitness, antifungal drug tolerance, and virulence of this major pathogen. Beyond the relevance to fungal pathogenesis, this work also provides new insight into the mitochondrial and cellular roles of the SAM complex in fungi. PMID:22286093

  3. Spot and Runway Departure Advisor (SARDA)

    NASA Technical Reports Server (NTRS)

    Jung, Yoon

    2016-01-01

    Spot and Runway Departure Advisor (SARDA) is a decision support tool to assist airline ramp controllers and ATC tower controllers to manage traffic on the airport surface to significantly improve efficiency and predictability in surface operations. The core function of the tool is the runway scheduler which generates an optimal solution for runway sequence and schedule of departure aircraft, which would minimize system delay and maximize runway throughput. The presentation also discusses the latest status of NASA's current surface research through a collaboration with an airline partner, where a tool is developed for airline ramp operators to assist departure pushback operations. The presentation describes the concept of the SARDA tool and results from human-in-the-loop simulations conducted in 2012 for Dallas-Ft. Worth International Airport and 2014 for Charlotte airport ramp tower.

  4. An Aggregation Advisor for Ligand Discovery.

    PubMed

    Irwin, John J; Duan, Da; Torosyan, Hayarpi; Doak, Allison K; Ziebart, Kristin T; Sterling, Teague; Tumanian, Gurgen; Shoichet, Brian K

    2015-09-10

    Colloidal aggregation of organic molecules is the dominant mechanism for artifactual inhibition of proteins, and controls against it are widely deployed. Notwithstanding an increasingly detailed understanding of this phenomenon, a method to reliably predict aggregation has remained elusive. Correspondingly, active molecules that act via aggregation continue to be found in early discovery campaigns and remain common in the literature. Over the past decade, over 12 thousand aggregating organic molecules have been identified, potentially enabling a precedent-based approach to match known aggregators with new molecules that may be expected to aggregate and lead to artifacts. We investigate an approach that uses lipophilicity, affinity, and similarity to known aggregators to advise on the likelihood that a candidate compound is an aggregator. In prospective experimental testing, five of seven new molecules with Tanimoto coefficients (Tc's) between 0.95 and 0.99 to known aggregators aggregated at relevant concentrations. Ten of 19 with Tc's between 0.94 and 0.90 and three of seven with Tc's between 0.89 and 0.85 also aggregated. Another three of the predicted compounds aggregated at higher concentrations. This method finds that 61 827 or 5.1% of the ligands acting in the 0.1 to 10 μM range in the medicinal chemistry literature are at least 85% similar to a known aggregator with these physical properties and may aggregate at relevant concentrations. Intriguingly, only 0.73% of all drug-like commercially available compounds resemble the known aggregators, suggesting that colloidal aggregators are enriched in the literature. As a percentage of the literature, aggregator-like compounds have increased 9-fold since 1995, partly reflecting the advent of high-throughput and virtual screens against molecular targets. Emerging from this study is an aggregator advisor database and tool ( http://advisor.bkslab.org ), free to the community, that may help distinguish between

  5. Senescence-accelerated mouse (SAM): a biogerontological resource in aging research.

    PubMed

    Takeda, T

    1999-01-01

    The senescence-accelerated mouse (SAM), consisting of 14 senescence-prone inbred strains (SAMP) and 4 senescence-resistant inbred strains (SAMR) has been under development since 1970 through the selective inbreeding of AKR/J strain mice donated by the Jackson laboratory in 1968, based on the data of the grading score of senescence, life span, and pathologic phenotypes. The characteristic feature of aging common to all SAMP and SAMR mice is accelerated senescence and normal aging, respectively. Furthermore, SAMP and SAMR strains manifest various pathobiological phenotypes which include such neurobiological phenotypes as deficits in learning and memory, emotional disorders, abnormal circadian rhythms, brain atrophy, hearing impairment, etc., and are often characteristic enough to differentiate the strains. Various efforts are currently being made using the SAM model to clarify the underlying mechanisms in accelerated senescence as well as the etiopathogenic mechanisms in age-associated pathobiologies. Genetic background and significance of SAM development are discussed. PMID:10537019

  6. AFM-assisted fabrication of thiol SAM pattern with alternating quantified surface potential

    PubMed Central

    2011-01-01

    Thiol self-assembled monolayers (SAMs) are widely used in many nano- and bio-technology applications. We report a new approach to create and characterize a thiol SAMs micropattern with alternating charges on a flat gold-coated substrate using atomic force microscopy (AFM) and Kelvin probe force microscopy (KPFM). We produced SAMs-patterns made of alternating positively charged, negatively charged, and hydrophobic-terminated thiols by an automated AFM-assisted manipulation, or nanografting. We show that these thiol patterns possess only small topographical differences as revealed by AFM, and distinguished differences in surface potential (20-50 mV), revealed by KPFM. The pattern can be helpful in the development of biosensor technologies, specifically for selective binding of biomolecules based on charge and hydrophobicity, and serve as a model for creating surfaces with quantified alternating surface potential distribution. PMID:21711703

  7. Obituary: Sam Roweis (1972-2010)

    NASA Astrophysics Data System (ADS)

    Hogg, David

    2011-12-01

    Computer scientist and statistical astronomer Sam Roweis took his own life in New York City on 2010 January 12. He was a brilliant and accomplished researcher in the field of machine learning, and a strong advocate for the use of computational statistics for automating discovery and scientific data analysis. He made several important contributions to astronomy and was working on adaptive astronomical data analysis at the time of his death. Roweis obtained his PhD in 1999 from the California Institute of Technology, where he worked on a remarkable range of subjects, including DNA computing, modeling of dynamical systems, signal processing, and speech recognition. During this time he unified and clarified some of the most important data analysis techniques, including Principal Component Analysis, Hidden Markov Models, and Expectation Maximization. His work was aimed at making data analysis and modeling faster, but also better justified scientifically. The last years of his PhD were spent in Princeton NJ, where he came in contact with a young generation of cosmologists thinking about microwave background and large-scale structure data. In a postdoc at University College London, Roweis co-created the Locally Linear Embedding (LLE) algorithm; a simple but flexible technique for mapping a large data set onto a low-dimensional manifold. The LLE paper obtained more than 2700 citations in 9 years, launched a new sub-field of machine learning known as "manifold learning," and inspired work in data visualization, search, and applied mathematics. In 2001, Roweis took a faculty job at the University of Toronto Computer Science Department. He continued working on data analysis methods that have probabilistic interpretation and therefore scientific applicability, but at the same time have good performance on large data sets. He was awarded a Sloan Fellowship, a Canada Research Chair, and a fellowship of the Canadian Institute for Advanced Research, among other honors and awards

  8. The design and implementation of a ventilator-management advisor.

    PubMed

    Rutledge, G W; Thomsen, G E; Farr, B R; Tovar, M A; Polaschek, J X; Beinlich, I A; Sheiner, L B; Fagan, L M

    1993-02-01

    VentPlan is an implementation of the architecture developed by the qualitative-quantitative (QQ) research group for combining qualitative and quantitative computation in a ventilator-management advisor (VMA). VentPlan calculates recommended settings for four controls of a ventilator by evaluating the predicted effects of alternative ventilator settings. A belief network converts clinical diagnoses to distributions on physiologic parameters. A mathematical-modeling module applies a patient-specific mathematical model of cardiopulmonary physiology to predict the effects of alternative ventilator settings. A decision-theoretic plan evaluator ranks the predicted effects of alternative ventilator settings according to a multiattribute-value model that specifies physician preferences for ventilator treatments. Our architecture allows VentPlan to interpret quantitative observations in light of the clinical context (such as the clinical diagnosis). We report a retrospective study of the ventilator-setting changes encountered in postoperative patients in a surgical intensive-care unit (ICU). We conclude that the QQ architecture allows VentPlan to apply a patient-specific physiologic model to calculate ventilator settings that are optimal with respect to a decision-theoretic value model describing physician preferences for setting the ventilator. PMID:8358487

  9. Contextualizing Gay-Straight Alliances: Student, Advisor, and Structural Factors Related to Positive Youth Development Among Members

    PubMed Central

    Poteat, V. Paul; Yoshikawa, Hirokazu; Calzo, Jerel P.; Gray, Mary L.; DiGiovanni, Craig. D.; Lipkin, Arthur; Mundy-Shephard, Adrienne; Perrotti, Jeff; Scheer, Jillian R.; Shaw, Mathew P.

    2014-01-01

    Gay-Straight Alliances (GSAs) may promote resilience. Yet, what GSA components predict wellbeing? Among 146 youth and advisors in 13 GSAs (58% lesbian, gay, bisexual, or questioning; 64% white; 38% received free/reduced-cost lunch), student (demographics, victimization, attendance frequency, leadership, support, control), advisor (years served, training, control) and contextual factors (overall support or advocacy, outside support for the GSA) that predicted purpose, mastery, and self-esteem were tested. In multilevel models, GSA support predicted all outcomes. Racial/ethnic minority youth reported greater wellbeing, yet lower support. Youth in GSAs whose advisors served longer and perceived more control and were in more supportive school contexts reported healthier outcomes. GSA advocacy also predicted purpose. Ethnographic notes elucidated complex associations and variability in how GSAs operated. PMID:25176579

  10. Contextualizing gay-straight alliances: student, advisor, and structural factors related to positive youth development among members.

    PubMed

    Poteat, V Paul; Yoshikawa, Hirokazu; Calzo, Jerel P; Gray, Mary L; DiGiovanni, Craig D; Lipkin, Arthur; Mundy-Shephard, Adrienne; Perrotti, Jeff; Scheer, Jillian R; Shaw, Matthew P

    2015-01-01

    Gay-straight alliances (GSAs) may promote resilience. Yet, what GSA components predict well-being? Among 146 youth and advisors in 13 GSAs (58% lesbian, gay, bisexual, or questioning; 64% White; 38% received free/reduced-cost lunch), student (demographics, victimization, attendance frequency, leadership, support, control), advisor (years served, training, control), and contextual factors (overall support or advocacy, outside support for the GSA) that predicted purpose, mastery, and self-esteem were tested. In multilevel models, GSA support predicted all outcomes. Racial/ethnic minority youth reported greater well-being, yet lower support. Youth in GSAs whose advisors served longer and perceived more control and were in more supportive school contexts reported healthier outcomes. GSA advocacy also predicted purpose. Ethnographic notes elucidated complex associations and variability as to how GSAs operated. PMID:25176579

  11. MMW, IR, and SAM signature collection

    NASA Astrophysics Data System (ADS)

    Reichstetter, Fred; Ward, Mary E.

    2002-08-01

    During the development of smart weapon's seeker/sensors, it is imperative to collect high quality signatures of the targets the system is intended to engage. These signatures are used to support algorithm development so the system can find and engage the targets of interest in the specific kill area on the target. Englin AFB FL is the AF development center for munitions; and in support of the development effort, the 46th Test Wing (46 TW) has initiated significant improvements in collection capabilities for signatures in the MMW, Infrared and Seismic, Acoustic and Magnetic (SAM) spectrum. Additionally, the Joint Munitions Test and Evaluation program office maintains a fleet of foreign ground vehicle targets used for such signature collection including items such as tanks, SCUD missile launchers, air defense units such as SA-06, SA-8, SA-13, and associated ground support trucks and general purpose vehicles. The major test facility includes a 300 ft tower used for mounting the instrumentation suite that currently includes, 10, 35 and 94 GHz MMW and 2-5(mu) and 8-12(mu) IR instrumentation systems. This facility has undergone major improvements in terms of background signature reduction, construction of a high bay building to house the turntable on which the targets are mounted, and an additional in- ground stationary turntable primarily for IR signature collection. Our experience using this facility to collect signatures for the smart weapons development community has confirmed a significant improvement in quality and efficiency. The need for the stationary turntable signature collection capability was driven by the requirements of the IR community who are interested in collecting signatures in clutter. This tends to be contrary to the MMW community that desires minimum background clutter. The resulting location, adjacent to the MMW tower, allows variations in the type and amount of clutter background that could be incorporated and also provides maximum utilization of

  12. Neal Lane: Confessions of a President's Science Advisor

    ScienceCinema

    Neal Lane

    2010-09-01

    Former science advisor to president Bill Clinton Neal Lane briefly reviews the history of the job of Science Advisor to the President and give some examples of issues he had to deal with when he was in that position, including climate change, stem cell research, the human genome, nanotechnology and research funding. He will also give his opinions about the present and future state of science in the U.S.

  13. Neal Lane: Confessions of a President's Science Advisor

    SciTech Connect

    Neal Lane

    2006-09-11

    Former science advisor to president Bill Clinton Neal Lane briefly reviews the history of the job of Science Advisor to the President and give some examples of issues he had to deal with when he was in that position, including climate change, stem cell research, the human genome, nanotechnology and research funding. He will also give his opinions about the present and future state of science in the U.S.

  14. Halomethane production in plants: Structure of the biosynthetic SAM-dependent halide methyltransferase from Arabidopsis thaliana**

    PubMed Central

    Schmidberger, Jason W.; James, Agata B.; Edwards, Robert; Naismith, James H.; O’Hagan, David

    2012-01-01

    A product structure of the halomethane producing enzyme in plants (Arabidopsis thaliana) is reported and a model for presentation of chloride/bromide ion to the methyl group of S-adenosyl-L-methionine (SAM) is presented to rationalise nucleophilic halide attack for halomethane production, gaseous natural products that are produced globally. PMID:20376845

  15. Experience with the shift technical advisor position

    SciTech Connect

    Melber, B.D.; Olson, J.; Schreiber, R.E.; Winges, L.

    1984-03-01

    The provision of engineering expertise on shift at commercial nuclear power plants has mainly taken the form of the Shift Technical Advisor (STA). This person, acting in a capacity that is part engineer and part operator, is expected to advise the operations crew in the event of an emergency and review plant operating experience during normal circumstances. The position was mandated by the Nuclear Regulatory Commission following the incident at Three Mile Island. This report expands on a growing body of knowledge regarding the effectiveness of the STA. The new data presented here come from interviews with plant personnel and utility officials from nine sites. Researchers from the Pacific Northwest Laboratory (PNL) interviewed plant personnel, including the STA and immediate management, the shift supervisor and management, the training department, and ancillary staff, all of whom affect the intended performance of the STA. The conclusions of the report are that the design of the STA position results in limited contribution during emergencies; more comprehensive ways should be sought to provide the variety and specificity of engineering expertise needed during such times.

  16. An intelligent advisor for the design manager

    NASA Technical Reports Server (NTRS)

    Rogers, James L.; Padula, Sharon L.

    1989-01-01

    A design problem is viewed as a complex system divisible into modules. Before the design of a complex system can begin, much time and money are spent in determining the couplings among modules and the presence of iterative loops. This is important because the design manager must know how to group the modules into substems and how to assign subsystems to design teams so that changes in one subsystem will have predictable effects on other subsystems. Determining these subsystems is not an easy, straightforward process and often important couplings are overlooked. Moreover, the planning task must be repeated as new information becomes available or as the design specifications change. The purchase of this research effort is to develop a knowledge-based tool to act as an intelligent advisor for the design manager. This tool identifies the subsystems of a complex design problem, orders them into a well-structured format, and marks the couplings among the subsystems to facilitate the use of multilevel tools. The tool was tested in the decomposition of the COFS (Control of Flexible Structures) mast design which has about 50 modules. This test indicated that this type of approach could lead to a substantial savings by organizing and displaying a complex problem as a sequence of subsystems easily divisible among design teams.

  17. Public Data at CeSAM

    NASA Astrophysics Data System (ADS)

    Moreau, C.; Le Brun, V.; Agneray, F.; Gimenez, S.; Roehlly, Y.

    2014-05-01

    Modern large observational programs produce important amounts of data from various origins, and need high level data-quality control, fast data access via easy-to-use graphic interfaces, as well as possibility to cross-correlate informations coming from different observations. The Centre de donneS Astrophysique de Marseille (CeSAM) has for mission to provide support to the teams in charge of the observational programs (specifications, software development and infrastructures), to produce and give the final data by proposing tools increasing their scientific value. We present here the various datasets hosted by the CeSAM under the ANIS environment. Even if mainly oriented towards large spectro-photometric extragalactic samples, we host as well an exoplanet transit database, or resolved galaxy data. The main datasets are those from the VVDS final release (45000 spectra), the Herschel extragalactic surveys (HerMES, HRS, GOODS-Herschel, VNGS), the CFHTLS-WIDE photometric redshifts catalog (17 millions objects), or the HST-COSMOS information system, that allows request from any of the 6 and access to the corresponding values in the other catalogs.

  18. Multiple polymer architectures of human Polyhomeotic homolog 3 (PHC3) SAM

    PubMed Central

    Nanyes, David R.; Junco, Sarah E.; Taylor, Alexander B.; Robinson, Angela K.; Patterson, Nicolle L.; Shivarajpur, Ambika; Halloran, Jonathan; Hale, Seth M.; Kaur, Yogeet; Hart, P. John; Kim, Chongwoo A.

    2014-01-01

    The self-association of sterile alpha motifs (SAMs) into a helical polymer architecture is a critical functional component of many different and diverse array of proteins. For the Drosophila Polycomb group (PcG) protein Polyhomeotic (Ph), its SAM polymerization serves as the structural foundation to cluster multiple PcG complexes, helping to maintain a silenced chromatin state. Ph SAM shares 64% sequence identity with its human ortholog, PHC3 SAM, and both SAMs polymerize. However, in the context of their larger protein regions, PHC3 SAM forms longer polymers compared to Ph SAM. Motivated to establish the precise structural basis for the differences, if any, between Ph and PHC3 SAM, we determined the crystal structure of the PHC3 SAM polymer. PHC3 SAM utilizes the same SAM-SAM interaction as the Ph SAM six-fold repeat polymer. Yet, PHC3 SAM polymerizes utilizing just five SAMs per turn of the helical polymer rather than the typical six per turn observed for all SAM polymers reported to date. Structural analysis suggested that malleability of the PHC3 SAM would allow formation of not just the five-fold repeat structure but possibly others. Indeed, a second PHC3 SAM polymer in a different crystal form forms a six-fold repeat polymer. These results suggest that the polymers formed by PHC3 SAM, and likely others, are quite dynamic. The functional consequence of the variable PHC3 SAM polymers may be to create different chromatin architectures. PMID:25044168

  19. Inhibition of Sam68 triggers adipose tissue browning.

    PubMed

    Zhou, Junlan; Cheng, Min; Boriboun, Chan; Ardehali, Mariam M; Jiang, Changfei; Liu, Qinghua; Han, Shuling; Goukassian, David A; Tang, Yao-Liang; Zhao, Ting C; Zhao, Ming; Cai, Lu; Richard, Stéphane; Kishore, Raj; Qin, Gangjian

    2015-06-01

    Obesity is associated with insulin resistance and type 2 diabetes; molecular mechanisms that promote energy expenditure can be utilized for effective therapy. Src-associated in mitosis of 68 kDa (Sam68) is potentially significant, because knockout (KO) of Sam68 leads to markedly reduced adiposity. In the present study, we sought to determine the mechanism by which Sam68 regulates adiposity and energy homeostasis. We first found that Sam68 KO mice have a significantly reduced body weight as compared to controls, and the difference is explained entirely by decreased adiposity. Interestingly, these effects were not mediated by a difference in food intake; rather, they were associated with enhanced physical activity. When they were fed a high-fat diet, Sam68 KO mice gained much less body weight and fat mass than their WT littermates did, and they displayed an improved glucose and insulin tolerance. In Sam68 KO mice, the brown adipose tissue (BAT), inguinal, and epididymal depots were smaller, and their adipocytes were less hypertrophied as compared to their WT littermates. The BAT of Sam68 KO mice exhibited reduced lipid stores and expressed higher levels of Ucp1 and key thermogenic and fatty acid oxidation genes. Similarly, depots of inguinal and epididymal white adipose tissue (WAT) in Sam68 KO mice appeared browner, their multilocular Ucp1-positive cells were much more abundant, and the expression of Ucp1, Cidea, Prdm16, and Ppargc1a genes was greater as compared to WT controls, which suggests that the loss of Sam68 also promotes WAT browning. Furthermore, in all of the fat depots of the Sam68 KO mice, the expression of M2 macrophage markers was up-regulated, and that of M1 markers was down-regulated. Thus, Sam68 plays a crucial role in controlling thermogenesis and may be targeted to combat obesity and associated disorders. PMID:25934704

  20. Inhibition of Sam68 triggers adipose tissue browning

    PubMed Central

    Zhou, Junlan; Cheng, Min; Boriboun, Chan; Ardehali, Mariam Mina; Jiang, Changfei; Liu, Qinghua; Han, Shuling; Goukassian, David A.; Tang, Yao-Liang; Zhao, Ting C.; Zhao, Ming; Cai, Lu; Richard, Stéphane; Kishore, Raj; Qin, Gangjian

    2015-01-01

    Obesity is associated with insulin resistance and type 2 diabetes; molecular mechanisms promoting energy expenditure may be utilized for effective therapy. Src-associated-in-mitosis-of-68kDa (Sam68) is potentially significant because knockout (KO) of Sam68 leads to markedly-reduced adiposity. Here we sought to determine the mechanism by which Sam68 regulates adiposity and energy homeostasis. We firstly found in Sam68-KO mice a significantly-reduced body weight with the difference explained entirely by decreased adiposity. Interestingly, these effects were not mediated by a difference in food intake, but rather associated with enhanced physical activity. When fed high-fat diet, Sam68-KO mice gained much lesser body weight and fat mass as compared to wild-type (WT) littermates and displayed an improved glucose and insulin tolerance. The brown adipose tissue (BAT), inguinal and epididymal depots are smaller and their adipocytes less hypertrophy in Sam68-KO mice than in WT littermates. The BAT of Sam68-KO mice exhibited reduced lipid stores and expressed higher levels of Ucp1 and key thermogenic and fatty-acid-oxidation genes. Similarly, depots of inguinal and epididymal white adipose tissue (WAT) in Sam68-KO mice appeared browner, their multilocular Ucp1-positive cells were much more abundant, and the expression of Ucp1, Cidea, Prdm16 and Ppargc1a genes was greater as compared to WT controls, suggesting that loss of Sam68 also promotes WAT browning. Furthermore, in all fat depots of Sam68-KO mice, the expression of M2 macrophage markers were upregulated and M1 markers downregulated. Thus Sam68 plays a crucial role in the control of thermogenesis and may be targeted to combat obesity and associated disorders. PMID:25934704

  1. Using SAM Assessment and Training for Office 2003

    ERIC Educational Resources Information Center

    Whittle, Gary

    2005-01-01

    This presentation will demonstrate the uses of SAM 2003 from Course Technology as a skills assessment and training software that is used via the Internet. Historically, testing in computer education has taken the form of pencil and paper or standardized testing. The actual computer skills of the student have not been properly assessed. With SAM,…

  2. [Anti-aging studies on the senescence accelerated mouse (SAM) strains].

    PubMed

    Takahashi, Ryoya

    2010-01-01

    Senescence accelerated mouse (SAM), a murine model of accelerated senescence, was established by Toshio Takeda and colleagues. SAM consists of series of SAMP (prone) and SAMR (resistant) lines. All SAMP lines (from SAMP1 to SAMP11) are characterized by accelerated accumulation of senile features, earlier onset and faster progress of age-associated pathological phenotypes, such as amyloidosis, impaired immune response, senile osteoporosis and deficits in learning and memory. These SAMP lines are useful for evaluation of putative anti-aging therapies. For example, SAMP1 line is used to study the anti-aging effect of the antioxidant containing foods and various anti-oxidants, such as coenzyme Q10, vitamin C, lycopene. SAMP8 line exhibiting an early onset of impaired learning and memory is often used for test strategies for therapeutic intervention of dementia of early onset. SAMP6 is used as an animal model for developing new strategies for the treatment of osteoporosis in humans. Various lines of SAM (P1, P6, P8, P10 and R1) are now commercially available for research. In this review, I will briefly introduce various usages of SAM in anti-aging research. PMID:20046059

  3. Development of an Expert Resource Network for Students and Advisors Engaging in Authentic Scientific Research

    NASA Astrophysics Data System (ADS)

    Danch, J. M.; Aker-bolin, K.

    2011-12-01

    In an effort to increase the the number of secondary students engaging in authentic scientific research and to improve the quality of that research, an online network of scientists and educators is being established. The Network will be assessable to students and advisors participating in the 2012 Monmouth Junior Science Symposium (MJSS), a scientific forum for New Jersey students, but is intended to serve as a model for use by participants in the additional 47 regional science symposia of which the MJSS is part. Hosted on the MJSS website, the Network will consist of contact information and profiles of scientists, educators and other research professionals willing to assist secondary students and their teacher/advisors perform authentic scientific research. Profiles will include area(s) of expertise and level of participation to which the individual is capable. The set of participating experts will have the potential to expand continually as additional professionals with a variety of backgrounds are recruited over time. The Network will also be made available to members of the New Jersey Science Supervisors Association to encourage school districts not currently participating in the MJSS to involve secondary students in conducting authentic scientific research. The Network's effectiveness will be evaluated by surveys completed by MJSS participants and their teacher/advisors included with their research paper submissions.

  4. Obituary: Sam Roweis (1972-2010)

    NASA Astrophysics Data System (ADS)

    Hogg, David

    2011-12-01

    Computer scientist and statistical astronomer Sam Roweis took his own life in New York City on 2010 January 12. He was a brilliant and accomplished researcher in the field of machine learning, and a strong advocate for the use of computational statistics for automating discovery and scientific data analysis. He made several important contributions to astronomy and was working on adaptive astronomical data analysis at the time of his death. Roweis obtained his PhD in 1999 from the California Institute of Technology, where he worked on a remarkable range of subjects, including DNA computing, modeling of dynamical systems, signal processing, and speech recognition. During this time he unified and clarified some of the most important data analysis techniques, including Principal Component Analysis, Hidden Markov Models, and Expectation Maximization. His work was aimed at making data analysis and modeling faster, but also better justified scientifically. The last years of his PhD were spent in Princeton NJ, where he came in contact with a young generation of cosmologists thinking about microwave background and large-scale structure data. In a postdoc at University College London, Roweis co-created the Locally Linear Embedding (LLE) algorithm; a simple but flexible technique for mapping a large data set onto a low-dimensional manifold. The LLE paper obtained more than 2700 citations in 9 years, launched a new sub-field of machine learning known as "manifold learning," and inspired work in data visualization, search, and applied mathematics. In 2001, Roweis took a faculty job at the University of Toronto Computer Science Department. He continued working on data analysis methods that have probabilistic interpretation and therefore scientific applicability, but at the same time have good performance on large data sets. He was awarded a Sloan Fellowship, a Canada Research Chair, and a fellowship of the Canadian Institute for Advanced Research, among other honors and awards

  5. Controller evaluations of the descent advisor automation aid

    NASA Technical Reports Server (NTRS)

    Tobias, Leonard; Volckers, Uwe; Erzberger, Heinz

    1989-01-01

    An automation aid to assist air traffic controllers in efficiently spacing traffic and meeting arrival times at a fix has been developed at NASA Ames Research Center. The automation aid, referred to as the descent advisor (DA), is based on accurate models of aircraft performance and weather conditions. The DA generates suggested clearances, including both top-of-descent point and speed profile data, for one or more aircraft in order to achieve specific time or distance separation objectives. The DA algorithm is interfaced with a mouse-based, menu-driven controller display that allows the air traffic controller to interactively use its accurate predictive capability to resolve conflicts and issue advisories to arrival aircraft. This paper focuses on operational issues concerning the utilization of the DA, specifically, how the DA can be used for prediction, intrail spacing, and metering. In order to evaluate the DA, a real time simulation was conducted using both current and retired controller subjects. Controllers operated in teams of two, as they do in the present environment; issues of training and team interaction will be discussed. Evaluations by controllers indicated considerable enthusiasm for the DA aid, and provided specific recommendations for using the tool effectively.

  6. Comparing Surfaces and Engineered Interfaces using Self-Assembled Monolayers (SAMs) and Injected SAMs Silanes

    SciTech Connect

    Morris, Mark J.; Simmons, Kevin L.

    2003-11-01

    The objective of this study was to show a comparison between property changes by formation of a self-assembled monolayer on the surface of PPG synthetic precipitated silica, which is a technique developed at PNNL, and by adding the SAMs silane chemical directly into the mixing bowl. These coatings have the potential to greatly increase the bond strength and enhance other properties between the particle and the rubber matrix of a rubber compound. Tensile testing measured peak stress and elongation at break. The increase in tensile strength shows how well the polymer-filler interfacial adhesion is doing. The study used five different SAM systems with a sulfur cured styrene butadiene rubber (SBR) tire rubber formulation. The three propylsilanes were propyl triethoxysilane, allyl triethoxysilane and 3-mercaptopropyl triethoxysilane. Five combinations of silanes were used in this study. The application of the silanes were 100% propyl triethoxy silane (100% Alkyl); a 10/90 mixture of allyl and propyl triethoxy silanes (10% vinyl/90% alkyl); a 50/50 mixture of the allyl and propyl (50% vinyl/50% alkyl); a 10/90mixture of 3-mercaptopropyl trimethoxysilane and propyl trimethoxysilane (10% mercaptan/90% alkyl) and lastly a 50/50 3-mercaptopropyl and propylsilanes (50% mercaptan/alkyl). The data not only shows improvement with SAMs, the peak stress data (ultimate strength) shows that the by changing the amount of silane content can change the physical properties

  7. SAM68 is a physiological regulator of SMN2 splicing in spinal muscular atrophy

    PubMed Central

    Pagliarini, Vittoria; Pelosi, Laura; Bustamante, Maria Blaire; Nobili, Annalisa; Berardinelli, Maria Grazia; D’Amelio, Marcello; Musarò, Antonio

    2015-01-01

    Spinal muscular atrophy (SMA) is a neurodegenerative disease caused by loss of motor neurons in patients with null mutations in the SMN1 gene. The almost identical SMN2 gene is unable to compensate for this deficiency because of the skipping of exon 7 during pre–messenger RNA (mRNA) processing. Although several splicing factors can modulate SMN2 splicing in vitro, the physiological regulators of this disease-causing event are unknown. We found that knockout of the splicing factor SAM68 partially rescued body weight and viability of SMAΔ7 mice. Ablation of SAM68 function promoted SMN2 splicing and expression in SMAΔ7 mice, correlating with amelioration of SMA-related defects in motor neurons and skeletal muscles. Mechanistically, SAM68 binds to SMN2 pre-mRNA, favoring recruitment of the splicing repressor hnRNP A1 and interfering with that of U2AF65 at the 3′ splice site of exon 7. These findings identify SAM68 as the first physiological regulator of SMN2 splicing in an SMA mouse model. PMID:26438828

  8. SAM Gcms Chromatography Performed at Mars : Elements of Interpretation

    NASA Astrophysics Data System (ADS)

    Szopa, C.; Coll, P. J.; Buch, A.; François, P.; Cabane, M.; Coscia, D.; Teinturier, S.; Navarro-Gonzalez, R.; Glavin, D. P.; Freissinet, C.; Mahaffy, P. R.

    2013-12-01

    The characterisation of the chemical and mineralogical composition of regolith samples collected with the Curiosity rover is a primary objective of the SAM experiment. These data should provide essential clues on the past habitability of Gale crater. Interpretation of the data collected after SAM pyrolysis evolved gas analysis (EGA) and gas chromatography mass spectrometry (GC-MS) experiments on the first soil samples collected by MSL at the Rocknest Aeolian Deposit in Gale Crater has been challenging due to the concomitant presence in the ovens of an oxychlorine phase present in the samples, and a derivatization agent coming from the SAM wet chemistry experiment (Glavin et al., 2013). Moreover, accurate identification and quantification, in the SAM EGA mode, of volatiles released from the heated sample, or generated by reactions occurring in the SAM pyrolysis oven, is also difficult for a few compounds due to evolution over similar temperature ranges and overlap of their MS signatures. Hence, the GC analyses, coupled with MS, enabled the separation and identification and quantification of most of the volatile compounds detected. These results can have been obtained through tests and calibration done with GC individual spare components and with the SAM testbed. This paper will present a view of the interpretation of the chromatograms obtained when analyzing the Rocknest and John Klein solid samples delivered to SAM, on sols 96 and 199 respectively, supported by laboratory calibrations.

  9. 76 FR 7593 - President's Council of Advisors on Science and Technology; Notice of Meeting: Partially Closed...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-10

    ... two years of undergraduate science, technology, engineering, and mathematics education. Additional... TECHNOLOGY POLICY President's Council of Advisors on Science and Technology; Notice of Meeting: Partially Closed Meeting of the President's Council of Advisors on Science and Technology ACTION: Public...

  10. 75 FR 11949 - Lincoln Investment Advisors Corporation and Lincoln Variable Insurance Products Trust; Notice of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-12

    ... COMMISSION Lincoln Investment Advisors Corporation and Lincoln Variable Insurance Products Trust; Notice of... requirements. Applicants: Lincoln Investment Advisors Corporation (``Adviser'') and Lincoln Variable Insurance..., One Granite Place, Concord, NH 03301 and Lincoln Variable Insurance Products Trust, 1300 S....

  11. 75 FR 47317 - President's Council of Advisors on Science and Technology

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-05

    ... topics of advanced manufacturing; science, technology, engineering, and mathematics (STEM) education; and... TECHNOLOGY POLICY President's Council of Advisors on Science and Technology Notice of Meeting: Partially Closed Meeting of the President's Council of Advisors on Science and Technology. ACTION: Public...

  12. When questions arise, physician advisors are there with the answers.

    PubMed

    2016-06-01

    DCH Health System's seven part-time physician advisors perform a variety of tasks to help ensure that the hospitals are appropriately reimbursed and that patients move smoothly through the continuum of care. They work with care managers on patient status and also assist with documentation, writing medical necessity appeals, resource utilization, and patient throughput. They attend daily transition rounds with the nurses, care managers, and social workers, and participate in weekly long-stay rounds and capacity rounds in the ICU and acute cardiac care. The health system brought in experts to educate the physician advisors, sent them to educational symposia, and pays for membership in professional organizations. PMID:27323508

  13. Beta test results for the CAA mini-SAM system

    SciTech Connect

    Johnson, R.C.; Monagle, M.

    1997-04-01

    The mission of the Chemical Analysis Automation (CAA) Program is to automate methods for chemical analysis of environmental samples. To accomplish this mission, the CAA team has developed automated laboratory systems based on a plug-and-work strategy for integrating components. Realizing that standardization is the key to implementing this strategy, CAA has developed, demonstrated, and encouraged commercialization of standards for laboratory automation. While the CAA mission is driven by the analyses in support of the extensive remediation programs of the Departments of Energy and Defense, it also impacts any industry that depends upon high volumes of repetitive chemical analysis. A Standard Analysis Method (SAM) is any collection of hardware and software used to automate part or all of a method. The method automated for the Mini-SAM testing is EPA Method 3550, which outlines semivolatiles extraction by sonication. The list of semivolatiles includes the polychlorinated biphenyl (PCB) analytes of interest. The basic building block of a SAM is the Standard Laboratory Module (SLM). For the Mini-SAM test an automated sonication SLM and an automated concentration SLM were configured to perform the extraction and concentration processes. The Mini-SAM differs from the Full-SAM in that a fully automated delivery of materials, samples, and extracts is not required. The intent of the Beta Test of the Mini-SAM was threefold. Firstly, the Mini-SAM Beta Test met a milestone mandated by the Department of Energy in the course of the program effort. Secondly, the CAA Program secured an independent assessment of the equipment and its capabilities from Assagai Analytical Laboratory. Lastly, the Program captured real-world sample data. The independent assessment, coupled with CAA observation of equipment performance, was used to determine strengths and weaknesses of the Mini-SAM and to compile possible modifications for CAA engineers to address.

  14. SAM 2 and SAGE data management and processing

    NASA Technical Reports Server (NTRS)

    Osborn, M. T.; Trepte, C. R.

    1987-01-01

    The data management and processing supplied by ST Systems Corporation (STX) for the Stratospheric Aerosol Measurement 2 (SAM 2) and Stratospheric Aerosol and Gas Experiment (SAGE) experiments for the years 1983 to 1986 are described. Included are discussions of data validation, documentation, and scientific analysis, as well as the archival schedule met by the operational reduction of SAM 2 and SAGE data. Work under this contract resulted in the archiving of the first seven years of SAM 2 data and all three years of SAGE data. A list of publications and presentations supported was also included.

  15. Recent Advances in Radical SAM Enzymology: New Structures and Mechanisms

    PubMed Central

    2015-01-01

    The radical S-adenosylmethionine (SAM) superfamily of enzymes catalyzes an amazingly diverse variety of reactions ranging from simple hydrogen abstraction to complicated multistep rearrangements and insertions. The reactions they catalyze are important for a broad range of biological functions, including cofactor and natural product biosynthesis, DNA repair, and tRNA modification. Generally conserved features of the radical SAM superfamily include a CX3CX2C motif that binds an [Fe4S4] cluster essential for the reductive cleavage of SAM. Here, we review recent advances in our understanding of the structure and mechanisms of these enzymes that, in some cases, have overturned widely accepted mechanisms. PMID:25009947

  16. Crystal structures of the SAM-III/S[subscript MK] riboswitch reveal the SAM-dependent translation inhibition mechanism

    SciTech Connect

    Lu, C.; Smith, A.M.; Fuchs, R.T.; Ding, F.; Rajashankar, K.; Henkin, T.M.; Ke, A.

    2010-01-07

    Three distinct classes of S-adenosyl-L-methionine (SAM)-responsive riboswitches have been identified that regulate bacterial gene expression at the levels of transcription attenuation or translation inhibition. The SMK box (SAM-III) translational riboswitch has been identified in the SAM synthetase gene in members of the Lactobacillales. Here we report the 2.2-{angstrom} crystal structure of the Enterococcus faecalis SMK box riboswitch. The Y-shaped riboswitch organizes its conserved nucleotides around a three-way junction for SAM recognition. The Shine-Dalgarno sequence, which is sequestered by base-pairing with the anti-Shine-Dalgarno sequence in response to SAM binding, also directly participates in SAM recognition. The riboswitch makes extensive interactions with the adenosine and sulfonium moieties of SAM but does not appear to recognize the tail of the methionine moiety. We captured a structural snapshot of the SMK box riboswitch sampling the near-cognate ligand S-adenosyl-L-homocysteine (SAH) in which SAH was found to adopt an alternative conformation and fails to make several key interactions.

  17. 17 CFR 4.14 - Exemption from registration as a commodity trading advisor.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... a commodity trading advisor. 4.14 Section 4.14 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION COMMODITY POOL OPERATORS AND COMMODITY TRADING ADVISORS General Provisions, Definitions and Exemptions § 4.14 Exemption from registration as a commodity trading advisor. This section...

  18. SADDLE HORSE AND OTHER LIVESTOCK ADVISORS' PERCEPTIONS OF 4-H CLUB WORK IN OHIO.

    ERIC Educational Resources Information Center

    GROVES, ROBERT H.

    PERCEPTIONS AND UNDERSTANDINGS OF 4-H OBJECTIVES AND PROGRAMS OF 4-H SADDLE HORSE ADVISORS WERE COMPARED WITH THOSE OF OTHER LIVESTOCK ADVISORS IN NORTHEASTERN AND SOUTHWESTERN DISTRICTS OF OHIO. DATA WERE COLLECTED BY QUESTIONNAIRES FROM 90 SADDLE HORSE AND 133 OTHER LIVESTOCK ADVISORS. STATE 4-H STAFF AND SUPERVISORS PROVIDED CORRECT ANSWERS.…

  19. 77 FR 4289 - President's Board of Advisors on Historically Black Colleges and Universities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-27

    ... President's Board of Advisors on Historically Black Colleges and Universities AGENCY: U.S. Department of Education, President's Board of Advisors on Historically Black Colleges and Universities (Board). ACTION... President's Board of Advisors on Historically Black Colleges and Universities. The notice also describes...

  20. 78 FR 31910 - President's Board of Advisors on Historically Black Colleges and Universities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-28

    ... President's Board of Advisors on Historically Black Colleges and Universities AGENCY: U.S. Department of Education, President's Board of Advisors on Historically Black Colleges and Universities (Board). ACTION... President's Board of Advisors on Historically Black Colleges and Universities. The notice also describes...

  1. 78 FR 57135 - President's Board of Advisors on Historically Black Colleges and Universities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-17

    ... President's Board of Advisors on Historically Black Colleges and Universities AGENCY: U.S. Department of Education, President's Board of Advisors on Historically Black Colleges and Universities. ACTION: Notice of... President's Board of Advisors on Historically Black Colleges and Universities. The notice also describes...

  2. 76 FR 30323 - President's Board of Advisors on Historically Black Colleges and Universities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-25

    ... President's Board of Advisors on Historically Black Colleges and Universities AGENCY: U.S. Department of Education, President's Board of Advisors on Historically Black Colleges and Universities (Board). ACTION... President's Board of Advisors on Historically Black Colleges and Universities. The notice also describes...

  3. 77 FR 74230 - PNC Capital Advisors, LLC, et al.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-13

    ... COMMISSION PNC Capital Advisors, LLC, et al.; Notice of Application December 6, 2012. AGENCY: Securities and... the Act to invest in certain financial instruments. Applicants: PNC Capital Advisors, LLC (``PNC Capital Advisors''), PNC Funds and PNC Advantage Funds (together, the ``Trusts''), and PNC...

  4. A Qualitative Inquiry into the Training and Development Provided to Community College Academic Advisors

    ERIC Educational Resources Information Center

    Mikluscak, George Steven, Jr.

    2010-01-01

    The qualitative study explored the training and development provided to Community College academic advisors who are members of the National Academic Advising Association (NACADA). The purpose was to investigate the factors academic advisors believe are crucial for the support of their roles as advisors. Professional, faculty, and self-identified…

  5. 77 FR 67808 - President's Council of Advisors on Science and Technology (PCAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-14

    ... President's Council of Advisors on Science and Technology (PCAST) AGENCY: Office of Science, Department of... summary agenda for a partially-closed meeting of the President's Council of Advisors on Science and... Advisors on Science and Technology (PCAST) is an advisory group of the Nation's leading scientists...

  6. 78 FR 12302 - President's Council of Advisors on Science and Technology (PCAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-22

    ... President's Council of Advisors on Science and Technology (PCAST) AGENCY: Office of Science, Department of... summary agenda for a partially closed meeting of the President's Council of Advisors on Science and... President's Council of Advisors on Science and Technology (PCAST) is an advisory group of the...

  7. 77 FR 26273 - President's Council of Advisors on Science and Technology (PCAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-03

    ... President's Council of Advisors on Science and Technology (PCAST) AGENCY: Department of Energy. ACTION... partially closed meeting of the President's Council of Advisors on Science and Technology (PCAST), and... INFORMATION: The President's Council of Advisors on Science and Technology (PCAST) is an advisory group of...

  8. 78 FR 68040 - President's Council of Advisors on Science and Technology Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-13

    ... President's Council of Advisors on Science and Technology Meeting AGENCY: Department of Energy. ACTION... partially-closed meeting of the President's Council of Advisors on Science and Technology (PCAST), and..., first-served basis. SUPPLEMENTARY INFORMATION: The President's Council of Advisors on ] Science...

  9. 78 FR 2259 - President's Council of Advisors on Science and Technology (PCAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-10

    ... President's Council of Advisors on Science and Technology (PCAST) AGENCY: Department of Energy. ACTION... teleconference call of the President's Council of Advisors on Science and Technology (PCAST), and describes the... of Advisors on Science and Technology (PCAST) is an advisory group of the nation's leading...

  10. 78 FR 35617 - President's Council of Advisors on Science and Technology (PCAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-13

    ... President's Council of Advisors on Science and Technology (PCAST) AGENCY: Office of Science, Department of... summary agenda for a partially-closed meeting of the President's Council of Advisors on Science and... Council of Advisors on Science and Technology (PCAST) is an advisory group of the nation's...

  11. 77 FR 51790 - President's Council of Advisors on Science and Technology (PCAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-27

    ... President's Council of Advisors on Science and Technology (PCAST) AGENCY: Department of Energy. ACTION... partially closed meeting of the President's Council of Advisors on Science and Technology (PCAST), and...: The President's Council of Advisors on Science and Technology (PCAST) is an advisory group of...

  12. 78 FR 50404 - President's Council of Advisors on Science and Technology (PCAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-19

    ... President's Council of Advisors on Science and Technology (PCAST) AGENCY: Office of Science, Department of... summary agenda for a partially-closed meeting of the President's Council of Advisors on Science and... Council of Advisors on Science and Technology (PCAST) is an advisory group of the nation's...

  13. 17 CFR 4.14 - Exemption from registration as a commodity trading advisor.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... a commodity trading advisor. 4.14 Section 4.14 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION COMMODITY POOL OPERATORS AND COMMODITY TRADING ADVISORS General Provisions, Definitions and Exemptions § 4.14 Exemption from registration as a commodity trading advisor. This section...

  14. 17 CFR 4.14 - Exemption from registration as a commodity trading advisor.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... a commodity trading advisor. 4.14 Section 4.14 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION COMMODITY POOL OPERATORS AND COMMODITY TRADING ADVISORS General Provisions, Definitions and Exemptions § 4.14 Exemption from registration as a commodity trading advisor. This section...

  15. 17 CFR 4.14 - Exemption from registration as a commodity trading advisor.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... a commodity trading advisor. 4.14 Section 4.14 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION COMMODITY POOL OPERATORS AND COMMODITY TRADING ADVISORS General Provisions, Definitions and Exemptions § 4.14 Exemption from registration as a commodity trading advisor. This section...

  16. Dyadic Perspectives on Advisor-Advisee Relationships in Counseling Psychology Doctoral Programs

    ERIC Educational Resources Information Center

    Schlosser, Lewis Z.; Kahn, Jeffrey H.

    2007-01-01

    The authors present the results from a study investigating advisor-advisee relationships in counseling psychology doctoral training. Participants were 47 advisor-advisee dyads who were currently working together in an advising relationship. The results indicated that advisors and advisees exhibited significant agreement in their assessments of…

  17. Future Farmers of America--A Profile of Chapters and Advisors.

    ERIC Educational Resources Information Center

    Gordon, Howard R. D.

    A study was conducted to collect information related to Future Farmers of America (FFA) advisors, chapters, and school characteristics. Specific objectives were to determine FFA activities influenced by the advisor's experience, and to identify factors perceived as contributing to the preparation of teachers for the role of advisor and to the…

  18. SAM II Data and Information (HDF-EOS)

    Atmospheric Science Data Center

    2016-09-01

    ... Data obtained from the Stratospheric Aerosol Measurement (SAM) II instrument, which flew on board the Nimbus-7 ... Spatial Resolution:  The altitude profiles of aerosol extinction have a 1 km vertical resolution. Temporal ...

  19. Curiosity Shakes, Bakes, and Tastes Mars with SAM

    NASA Video Gallery

    NASA's Curiosity rover analyzed its first solid sample of Mars with a variety of instruments, including the Sample Analysis at Mars (SAM) instrument suite. Developed at NASA's Goddard Space Flight ...

  20. In-Orbit Calibration of a SAMS Triaxial Sensor Head

    NASA Technical Reports Server (NTRS)

    Chestney, Louis S.; Sicker, Ronald J.

    1996-01-01

    This report describes the results of in orbit calibration data collected for a Space Acceleration Measurement System (SAMS) Triaxial Sensor Head (TS H) and the methods used to process the data for bias and gravity levels.

  1. Knowledge for Practice: A Training Program for College Access Advisors

    ERIC Educational Resources Information Center

    Tracy, Elizabeth M.; Freimark, Susan; Boss, Maria Isabel; Lonergan, Patricia

    2005-01-01

    Social and environmental factors, unique to many first-generation college students and other at-risk youth, complicate the job of college advising. This study provides program design, and teaching topics and techniques to further sensitize advisors to social, emotional, and cultural factors that impact their work.

  2. Database Advisor: A New Tool for K-12 Research Projects.

    ERIC Educational Resources Information Center

    Berteaux, Susan S.; Strong, Sandra S.

    The Database Advisor (DBA) is a tool designed to guide users to the most appropriate World Wide Web-based databases for their research. Developed in 1997 by the Science Libraries at the University of California, San Diego (UCSD), DBA is a Web-based front-end to bibliographic and full-text databases to which UCSD has remote access. DBA allows the…

  3. Increasing Advisor Effectiveness by Understanding Conflict and Conflict Resolution

    ERIC Educational Resources Information Center

    McClellan, Jeffrey

    2005-01-01

    On a daily basis, advisors encounter various types of interpersonal and intrapersonal conflict. Through this article, the reader will better understand conflict, its positive and negative impacts and the approaches of the actors experiencing conflict, and the means whereby conflicts arise, escalate, and come to resolution in advising situations.…

  4. The Advising Connection: A Training Program for Faculty Advisors.

    ERIC Educational Resources Information Center

    Clifton, Cherie; Long, Carolyne

    At Amarillo College (AC) in Texas, academic advising is provided by certified counselors at the Advising and Counseling Center in conjunction with faculty and staff of the developmental studies department. In addition, all teaching faculty serve as student advisors. During the past 5 years, a number of factors have placed increased demands on the…

  5. Role Change (From Teacher to Teacher/Advisor).

    ERIC Educational Resources Information Center

    Aiken, James B.

    Cognitive and operational difficulties that may ensue from the modification of teacher role into teacher/advisor role can be alleviated by the adoption of four sequential processes. Teachers and administrators involved in role change must pass through the stages of: (1) modifying the old role to include the new; (2) identifying with the new role…

  6. Risk Management in College Fraternities: Guidance from Two Faculty Advisors

    ERIC Educational Resources Information Center

    Rosenberg, Stuart; Mosca, Joseph

    2016-01-01

    Students who become members of fraternities and sororities avail themselves of significant opportunities that enrich their college life. Faculty advisors play an important role in assisting fraternities and sororities in shaping students' leadership, scholastic, and personal development. Given the risks such as alcohol use and hazing that continue…

  7. New Routes To Transfer. The Advisor. November 2001

    ERIC Educational Resources Information Center

    British Columbia Council on Admissions and Transfer, 2001

    2001-01-01

    The Advisor provides important information or "system alerts," in user-friendly format and language, on issues and changes affecting admissions and transfer. This document outlines new transfer possibilities in academic arts and science programs, and business diploma programs -- the two largest programs offered by the colleges, university colleges…

  8. Advisors' Perceptions of Community College Advising

    ERIC Educational Resources Information Center

    Solis, Linda Yolanda

    2012-01-01

    The purpose of the following dissertation was to gain understanding of community college advisors' perceptions of the community college advising process. A constructivist approach was utilized to develop a general description and understanding of what influences advising perceptions from qualitative data collected through interviews with…

  9. Trends in Defamation Law: Let the Advisor Beware.

    ERIC Educational Resources Information Center

    King, Robert D.

    1992-01-01

    Although most lawsuits brought by students against a college are grounded on contract and due process theories, courts have been willing to consider students' defamation suits using tort theories. Recent changes in defamation law have engendered increased litigation and risk of liability for faculty and advisors. (Author/MSE)

  10. Student Preferences for Academic Advisors as Transformational Leaders

    ERIC Educational Resources Information Center

    Drozd, Deborah Suzanne

    2010-01-01

    A quantitative study was conducted to determine the preferences of undergraduate students for academic advisors as transformational leaders. A questionnaire was developed and distributed to undergraduate students enrolled in leadership classes at a major land grant university to determine importance ratings based on the variables including…

  11. Training Mentors as Educational Advisors for Adult Learners. Process Manual.

    ERIC Educational Resources Information Center

    Singer, Elizabeth W.

    Materials used by the director of a project designed to assist key personnel in businesses, industries, and agencies with the educational counseling of their employees are presented. The objectives of the project, "Training Mentors as Educational Advisors of Adult Learners," were to: (1) provide two training sessions in educational counseling…

  12. LIA: An Intelligent Advisor for E-Learning

    ERIC Educational Resources Information Center

    Capuano, Nicola; Gaeta, Matteo; Marengo, Agostino; Miranda, Sergio; Orciuoli, Francesco; Ritrovato, Pierluigi

    2009-01-01

    Intelligent e-learning systems have revolutionized online education by providing individualized and personalized instruction for each learner. Nevertheless, until now very few systems were able to leave academic laboratories and be integrated into real commercial products. One of these few exceptions is the Learning Intelligent Advisor (LIA)…

  13. Shifting Roles: From Language Teachers to Learning Advisors

    ERIC Educational Resources Information Center

    Morrison, Brian R.; Navarro, Diego

    2012-01-01

    Although learning advisors are often qualified teachers, the skills they apply, such as those discussed by Kelly (1996), require a significant shift in approach regarding interaction with students. As teachers reorient themselves to advising, their role changes quite markedly from teaching language to advising on learning (Mozzon-McPherson, 2001).…

  14. 76 FR 80733 - Extension of Temporary Registration of Municipal Advisors

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-27

    .... \\6\\ See Securities Exchange Act Release No. 62824, 75 FR 54465 (September 8, 2010) (``Interim Release.... 63576; 76 FR 824 (January 6, 2011) (``Proposing Release''). \\8\\ See 17 CFR 240.15Ba2-6T(e). \\9\\ See 17... COMMISSION 17 CFR Part 240 RIN 3235-AK69 Extension of Temporary Registration of Municipal Advisors...

  15. Auxiliary iron-sulfur cofactors in radical SAM enzymes.

    PubMed

    Lanz, Nicholas D; Booker, Squire J

    2015-06-01

    A vast number of enzymes are now known to belong to a superfamily known as radical SAM, which all contain a [4Fe-4S] cluster ligated by three cysteine residues. The remaining, unligated, iron ion of the cluster binds in contact with the α-amino and α-carboxylate groups of S-adenosyl-l-methionine (SAM). This binding mode facilitates inner-sphere electron transfer from the reduced form of the cluster into the sulfur atom of SAM, resulting in a reductive cleavage of SAM to methionine and a 5'-deoxyadenosyl radical. The 5'-deoxyadenosyl radical then abstracts a target substrate hydrogen atom, initiating a wide variety of radical-based transformations. A subset of radical SAM enzymes contains one or more additional iron-sulfur clusters that are required for the reactions they catalyze. However, outside of a subset of sulfur insertion reactions, very little is known about the roles of these additional clusters. This review will highlight the most recent advances in the identification and characterization of radical SAM enzymes that harbor auxiliary iron-sulfur clusters. This article is part of a Special Issue entitled: Fe/S proteins: Analysis, structure, function, biogenesis and diseases. PMID:25597998

  16. Tangled in a Complex Web of Relationships: Athletic/Academic Advisors Negotiating Privacy Disclosure Warnings with College Student-Athletes

    ERIC Educational Resources Information Center

    Thompson, Jason

    2013-01-01

    To investigate privacy management in the relationship between athletic/academic advisors and college student-athletes, I interviewed 37 advisors to address the following questions: What type of privacy rules do student-athletes communicate to advisors? How do advisors manage student-athletes' private information following these rules?…

  17. SAMS Acceleration Measurements on Mir from June to November 1995

    NASA Technical Reports Server (NTRS)

    DeLombard, Richard; Hrovat, Ken; Moskowitz, Milton; McPherson, Kevin

    1996-01-01

    The NASA Microgravity Science and Applications Division (MSAD) sponsors science experiments on a variety of microgravity carriers, including sounding rockets, drop towers, parabolic aircraft, and Orbiter missions. The MSAD sponsors the Space Acceleration Measurement System (SAMS) to support microgravity science experiments with acceleration measurements to characterize the microgravity environment to which the experiments were exposed. The Principal Investigator Microgravity Services project at the NASA Lewis Research Center supports principal investigators of microgravity experiments as they evaluate the effects of varying acceleration levels on their experiments. In 1993, a cooperative effort was started between the United States and Russia involving science utilization of the Russian Mir space station by scientists from the United States and Russia. MSAD is currently sponsoring science experiments participating in the Shuttle-Mir Science Program in cooperation with the Russians on the Mir space station. Included in the complement of MSAD experiments and equipment is a SAMS unit In a manner similar to Orbiter mission support, the SAMS unit supports science experiments from the U.S. and Russia by measuring the microgravity environment during experiment operations. The initial SAMS supported experiment was a Protein Crystal Growth (PCG) experiment from June to November 1995. SAMS data were obtained during the PCG operations on Mir in accordance with the PCG Principal Investigator's requirements. This report presents an overview of the SAMS data recorded to support this PCG experiment. The report contains plots of the SAMS 100 Hz sensor head data as an overview of the microgravity environment, including the STS-74 Shuttle-Mir docking.

  18. The meteorological advisor in a nuclear generation station emergency plan

    SciTech Connect

    Caiazza, R.

    1985-01-01

    Niagara Mohawk Power Corporation (NMPC) has developed an extensive emergency response plan for the Nine Mile Point Nuclear Generating Station, located near Oswego, New York, in response to requirements of the Nuclear Regulatory Commission (NRC). If an emergency involving actual or potential release of radioactivity occurs, meteorological conditions in the vicinity of the plant are an extremely important factor in the emergency response. In recognition of this, NMPC has included a Meteorological Advisor position in its Technical Support Center (TSC)/Emergency Operations Facility (HOF) support staffing plans. The Meteorological Advisor is responsible for verification of meteorological measurements, interpretation and dissemination of weather forecasts, dose projection verification, and monitoring team direction. This paper describes those responsibilities as they are integrated into the emergency plan.

  19. SAM68: Signal Transduction and RNA Metabolism in Human Cancer

    PubMed Central

    Frisone, Paola; Pradella, Davide; Di Matteo, Anna; Belloni, Elisa; Ghigna, Claudia; Paronetto, Maria Paola

    2015-01-01

    Alterations in expression and/or activity of splicing factors as well as mutations in cis-acting splicing regulatory sequences contribute to cancer phenotypes. Genome-wide studies have revealed more than 15,000 tumor-associated splice variants derived from genes involved in almost every aspect of cancer cell biology, including proliferation, differentiation, cell cycle control, metabolism, apoptosis, motility, invasion, and angiogenesis. In the past decades, several RNA binding proteins (RBPs) have been implicated in tumorigenesis. SAM68 (SRC associated in mitosis of 68 kDa) belongs to the STAR (signal transduction and activation of RNA metabolism) family of RBPs. SAM68 is involved in several steps of mRNA metabolism, from transcription to alternative splicing and then to nuclear export. Moreover, SAM68 participates in signaling pathways associated with cell response to stimuli, cell cycle transitions, and viral infections. Recent evidence has linked this RBP to the onset and progression of different tumors, highlighting misregulation of SAM68-regulated splicing events as a key step in neoplastic transformation and tumor progression. Here we review recent studies on the role of SAM68 in splicing regulation and we discuss its contribution to aberrant pre-mRNA processing in cancer. PMID:26273626

  20. Ensuring GRID resource availability with the SAM framework in LHCb

    NASA Astrophysics Data System (ADS)

    Closier, J.; Paterson, S.; Santinelli, R.

    2008-07-01

    The LHCb experiment has chosen to use the SAM framework (Service Availability Monitoring Environment from EGEE-II) [1] make extensive tests of the LHCb environment at all the accessible grid resources. The availability and the proper definition of the local Computing and Storage Elements, user interfaces as well as the WLCG software environment are checked. The SAM framework is also used to pre-install the LHCb applications in the shared software area provided by each site. The deployment of the LHCb applications is based on a python tool developed inside the experiment. It is used for software management including incremental installation of interdependent packages and clean package removal. After the application software is installed a validation test of the whole MC chain is run. According to the results of the experiment specific SAM tests, the sites are (re)integrated into the LHCb production system managed by DIRAC [2]. The possibility of automated dynamic site certification using the SAM test suite is explored. This paper will describe the various ways of the LHCb use of the SAM framework. Practical experience in the recent production runs, current limitations and future developments will be presented.

  1. NO{sub X} advisor: Intelligent software for combustion optimization

    SciTech Connect

    Pfahler, J.; Eskenazi, D; Levy, E.

    1996-01-01

    Under a Tailored Collaboration Project funded by PEPCO and EPRI, Lehigh University`s Energy Research Center has been developing software for use by plant personnel to optimize a pulverized coal-fired boiler to reduce NO{sub x} emissions and minimize heat rate. Based on expert systems, neural networks and a mathematical optimization algorithm, the NO{sub x} Advisor uses the expert system to safely guide the plant engineer through a series of parametric boiler tests, gathering a database to characterize the operation of the boiler over a wide range of conditions. The neural network develops non-linear mappings between NO{sub x} heat rate and the controllable parameters. These are then used by the mathematical optimization algorithm to identify optimal operating conditions. The Advisor can be used to adjust a boiler to reach minimum NO{sub x} or identify the conditions that give minimum heat rate subject to a target NO{sub x} value. The Advisor operates off-line on a PC platform, gathering data automatically from the plant data highway and/or manually as needed. Results of a field trial at PEPCO`s Potomac River Station are described.

  2. A Sophomore-to-Junior Mentoring Program that Works: The SAM Program at the University of Texas Pan American

    ERIC Educational Resources Information Center

    Reyes, Maria Elena

    2012-01-01

    In an effort to increase the retention rates of college sophomores in a Hispanic Serving Institution in south Texas, university administrators created and implemented a successful peer mentoring model, the Sophomore Academic Mentoring (SAM) Program. Results suggest that the program has contributed to an increase in retention for second year,…

  3. Sarm1-mediated axon degeneration requires both SAM and TIR interactions.

    PubMed

    Gerdts, Josiah; Summers, Daniel W; Sasaki, Yo; DiAntonio, Aaron; Milbrandt, Jeffrey

    2013-08-14

    Axon degeneration is an evolutionarily conserved pathway that eliminates damaged or unneeded axons. Manipulation of this poorly understood pathway may allow treatment of a wide range of neurological disorders. In an RNAi-based screen performed in cultured mouse DRG neurons, we observed strong suppression of injury-induced axon degeneration upon knockdown of Sarm1 [SARM (sterile α-motif-containing and armadillo-motif containing protein)]. We find that a SARM-dependent degeneration program is engaged by disparate neuronal insults: SARM ablation blocks axon degeneration induced by axotomy or vincristine treatment, while SARM acts in parallel with a soma-derived caspase-dependent pathway following trophic withdrawal. SARM is a multidomain protein that associates with neuronal mitochondria. Deletion of the N-terminal mitochondrial localization sequence disrupts SARM mitochondrial localization in neurons but does not alter its ability to promote axon degeneration. In contrast, mutation of either the SAM (sterile α motif) or TIR (Toll-interleukin-1 receptor) domains abolishes the ability of SARM to promote axonal degeneration, while a SARM mutant containing only these domains elicits axon degeneration and nonapoptotic neuronal death even in the absence of injury. Protein-protein interaction studies demonstrate that the SAM domains are necessary and sufficient to mediate SARM-SARM binding. SARM mutants lacking a TIR domain bind full-length SARM and exhibit strong dominant-negative activity. These results indicate that SARM plays an integral role in the dismantling of injured axons and support a model in which SAM-mediated multimerization is necessary for TIR-dependent engagement of a downstream destruction pathway. These findings suggest that inhibitors of SAM and TIR interactions represent therapeutic candidates for blocking pathological axon loss and neuronal cell death. PMID:23946415

  4. SAM-T08, HMM-based protein structure prediction

    PubMed Central

    Karplus, Kevin

    2009-01-01

    The SAM-T08 web server is a protein structure prediction server that provides several useful intermediate results in addition to the final predicted 3D structure: three multiple sequence alignments of putative homologs using different iterated search procedures, prediction of local structure features including various backbone and burial properties, calibrated E-values for the significance of template searches of PDB and residue–residue contact predictions. The server has been validated as part of the CASP8 assessment of structure prediction as having good performance across all classes of predictions. The SAM-T08 server is available at http://compbio.soe.ucsc.edu/SAM_T08/T08-query.html PMID:19483096

  5. Description of ferrocenylalkylthiol SAMs on gold by molecular dynamics simulations.

    PubMed

    Goujon, F; Bonal, C; Limoges, B; Malfreyt, P

    2009-08-18

    Molecular dynamics simulations of mixed monolayers consisting of Fc(CH2)12S-/C10S-Au SAMs are carried out to calculate structural (density profiles, angular distributions, positions of atoms) and energetic properties. The purpose of this paper is to explore the possible inhomogeneity of the neutral ferrocene moieties within the monolayer. Five systems have been studied using different grafting densities for the ferrocenylalkylthiolates. The angular distributions are described in terms of the relative contributions from isolated and clustered ferrocene moieties in the binary SAMs. It is shown that the energetic contributions strongly depend on the state of the ferrocene. The ability of molecular dynamics simulations to enable better understanding the SAM structure is illustrated in this work. PMID:19449821

  6. The methylthiolation reaction mediated by the Radical-SAM enzymes

    PubMed Central

    Atta, Mohamed; Arragain, Simon; Fontecave, Marc; Mulliez, Etienne; Hunt, John F.; Luff, Jon D.; Forouhar, Farhad

    2014-01-01

    Over the past ten years, considerable progress has been made in our understanding of the mechanistic enzymology of the Radical-SAM enzymes. It is now clear that these enzymes appear to be involved in a remarkably wide range of chemically challenging reactions. This review article highlights mechanistic and structural aspects of the methylthiotransferases (MTTases) sub-class of the Radical-SAM enzymes. The mechanism of methylthio insertion, now observed to be performed by three different enzymes is an exciting unsolved problem. PMID:22178611

  7. VAS: A Vision Advisor System combining agents and object-oriented databases

    NASA Technical Reports Server (NTRS)

    Eilbert, James L.; Lim, William; Mendelsohn, Jay; Braun, Ron; Yearwood, Michael

    1994-01-01

    A model-based approach to identifying and finding the orientation of non-overlapping parts on a tray has been developed. The part models contain both exact and fuzzy descriptions of part features, and are stored in an object-oriented database. Full identification of the parts involves several interacting tasks each of which is handled by a distinct agent. Using fuzzy information stored in the model allowed part features that were essentially at the noise level to be extracted and used for identification. This was done by focusing attention on the portion of the part where the feature must be found if the current hypothesis of the part ID is correct. In going from one set of parts to another the only thing that needs to be changed is the database of part models. This work is part of an effort in developing a Vision Advisor System (VAS) that combines agents and objected-oriented databases.

  8. AIDA: an automated insulin dosage advisor.

    PubMed Central

    Lehmann, E. D.; Deutsch, T.

    1992-01-01

    A prototype computer system utilising a model of carbohydrate metabolism linked to an expert system is described. The prototype which integrates quantitative and qualitative computational methodologies can be used to predict blood glucose profiles and adjust insulin doses in type I diabetic subjects. PMID:1482998

  9. Radical-Mediated Enzymatic Methylation: A Tale of Two SAMS

    PubMed Central

    2011-01-01

    Methylation is an essential and ubiquitous reaction that plays an important role in a wide range of biological processes. Most biological methylations use S-adenosylmethionine (SAM) as the methyl donor and proceed via an SN2 displacement mechanism. However, researchers have discovered an increasing number of methylations that involve radical chemistry. The enzymes known to catalyze these reactions all belong to the radical SAM superfamily. This family of enzymes utilizes a specialized [4Fe-4S] cluster for reductive cleavage of SAM to yield a highly reactive 5'-deoxyadenosyl (dAdo) radical. Radical chemistry is then imposed on a variety of organic substrates, leading to a diverse array of transformations. Until recently, researchers had not fully understood how these enzymes employ radical chemistry to mediate a methyl transfer reaction. Sequence analyses reveal that the currently identified radical SAM methyltransferases (RSMTs) can be grouped into three classes, which appear distinct in protein architecture and mechanism. Class A RSMTs mainly include the rRNA methyltransferases RlmN and Cfr from various origins. As exemplified by Escherichia coli RlmN, these proteins have a single canonical radical SAM core domain that includes an (βα)6 partial barrel most similar to that of pyruvate formate lyase-activase. The exciting recent studies on RlmN and Cfr are beginning to provide insights into the intriguing chemistry of class A RSMTs. These enzymes utilize a methylene radical generated on a unique methylated cysteine residue. However, based on the variety of substrates used by the other classes of RSMTs, alternative mechanisms are likely to be discovered. Class B RSMTs contain a proposed N-terminal cobalamin binding domain in addition to a radical SAM domain at the C-terminus. This class of proteins methylates diverse substrates at inert sp3 carbons, aromatic heterocycles, and phosphinates, possibly involving a cobalamin-mediated methyl transfer process. Class C

  10. EFFECTS OF POLYCYCLIC AROMATIC HYDROCARBON OF SAM-COATED ELECTRODES USING FERRYICYANIDE AS THE REDOX INDICATOR

    EPA Science Inventory

    Electrochemical responses on self-assembled monolayer (SAM)-coated polycrystalline gold electrodes were investigated using cyclic voltammetry and square wave voltammetry with a three electrode system. Experimental results show potential in the application of pyrene-imprinted SAM...

  11. SAMS Acceleration Measurement on Mir From March to September 1996

    NASA Technical Reports Server (NTRS)

    Moskowitz, Milton E.; Hrovat, Ken; Truong, Duc; Reckart, Timothy

    1997-01-01

    During NASA Increment 2 (March to September 1996), over 15 gigabytes of acceleration data were collected by the Space Acceleration Measurement System (SAMS) onboard the Russian Space Station, Mir. The data were recorded on 55 optical disks and were returned to Earth on STS-79. During this time, SAMS data were collected in the Kristall and Kvant modules, and in the Priroda module to support the following experiments: the Queen's University Experiments in Liquid Diffusion (QUELD), the Technological Evaluation of the MIM (TEM), the Forced Flow Flame Spreading Test (FFFT), and Candle Flames in Microgravity (CFM). This report points out some of the salient features of the microgravity environment to which these experiments were exposed. Also documented are mission events of interest such as the docked phase of STS-76 operations, an extravehicular activity (EVA) to install and deploy solar panels on the Kvant module, a Progress engine burn to raise Mir's altitude, and an on-orbit SAMS calibration procedure. Also included are a description of the Mir module orientations, and the panel notations within the modules. This report presents an overview of the SAMS acceleration measurements recorded by 10 Hz and 100 Hz sensor heads. Variations in the acceleration environment caused by unique activities such as crew exercise and life-support fans are presented. The analyses included herein complement those presented in previous mission summary reports published by the Principal Investigator Microgravity Services (PIMS) group.

  12. Social Activity Method (SAM): A Fractal Language for Mathematics

    ERIC Educational Resources Information Center

    Dowling, Paul

    2013-01-01

    In this paper I shall present and develop my organisational language, "social activity method" (SAM), and illustrate some of its applications. I shall introduce a new scheme for "modes of recontextualisation" that enables the analysis of the ways in which one activity--which might be school mathematics or social research or any…

  13. Information System through ANIS at CeSAM

    NASA Astrophysics Data System (ADS)

    Moreau, C.; Agneray, F.; Gimenez, S.

    2015-09-01

    ANIS (AstroNomical Information System) is a web generic tool developed at CeSAM to facilitate and standardize the implementation of astronomical data of various kinds through private and/or public dedicated Information Systems. The architecture of ANIS is composed of a database server which contains the project data, a web user interface template which provides high level services (search, extract and display imaging and spectroscopic data using a combination of criteria, an object list, a sql query module or a cone search interfaces), a framework composed of several packages, and a metadata database managed by a web administration entity. The process to implement a new ANIS instance at CeSAM is easy and fast : the scientific project has to submit data or a data secure access, the CeSAM team installs the new instance (web interface template and the metadata database), and the project administrator can configure the instance with the web ANIS-administration entity. Currently, the CeSAM offers through ANIS a web access to VO compliant Information Systems for different projects (HeDaM, HST-COSMOS, CFHTLS-ZPhots, ExoDAT,...).

  14. Skill Acquisition Measures (SAM). Elementary Mathematics Level IV. Teacher's Manual.

    ERIC Educational Resources Information Center

    Lancaster City School District, PA.

    The Skill Acquisition Measures (SAM) were designed by the Lancaster (Pennsylvania) School District as criterion referenced tests for mathematics. This manual consists of copies of the student test forms for level 4, with additional information for the teacher's use. Each of the test items is presented with the correct answers and the criteria for…

  15. Yield estimation from hyperspectral imagery using Spectral Angle Mapper (SAM)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Vegetation indices (VIs) derived from remotely sensed imagery are commonly used to estimate crop yields. Spectral angle mapper (SAM) provides an alternative approach to quantifying the spectral differences among all pixels in imagery and therefore has the potential for mapping yield variability. The...

  16. 78 FR 47695 - Sam Rayburn Dam Power Rate

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-06

    ... September 30, 2013 (77 FR 67813, November. 14, 2012). The Administrator, Southwestern Power Administration (Southwestern), has prepared Current and Revised 2013 Power Repayment Studies which show the need for an... Southwestern Power Administration Sam Rayburn Dam Power Rate AGENCY: Southwestern Power Administration,...

  17. 77 FR 67813 - Sam Rayburn Dam Project Power Rate

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-14

    ... Rayburn power rate extension were announced by a Federal Register (77 FR 50493) notice published on August... published notice in the Federal Register, (77 FR 50493), of the proposed rate extension for the Rayburn... Southwestern Power Administration Sam Rayburn Dam Project Power Rate AGENCY: Southwestern Power...

  18. Skill Acquisition Measures (SAM). Elementary Mathematics Level V. Teacher's Manual.

    ERIC Educational Resources Information Center

    Lancaster City School District, PA.

    The Skill Acquisition Measures (SAM) were designed by the Lancaster (Pennsylvania) School District as criterion referenced tests for mathematics. This manual consists of copies of the student test forms for level 5, with additional information for the teacher's use. Each of the test items is presented with the correct answers and the criteria for…

  19. Dynamics within alkylsiloxane SAMs studied by sensitive dielectric spectroscopy

    NASA Astrophysics Data System (ADS)

    Scott, Mary; Stevens, Derrick; Bochinski, Jason; Clarke, Laura

    2009-03-01

    Self assembled monolayers (SAMs) are a ubiquitous tool in modern research and their static structure has been extensively studied. Fewer investigations have addressed dynamics within these systems; however, such motions within SAMs will affect surface properties such as friction and blocking ability (permeability). In this study, sensitive, dielectric spectroscopy over a broad temperature range (4-400 K) has been employed to study relaxations within planar alkylsiloxane SAMs[1] . Highly disordered SAMs of varying density were grown by vapor deposition. Two dielectric relaxations were observed. The first, a polyethylene-like relaxation similar to that previously reported in phase-segregated alkyl side-chain polymers, is observed for all films with alkyl chains containing four or more carbons. This is an interacting or glassy relaxation. The second motion, which is observable only at high film densities, is a local mode, which follows an Arrhenius dependence on temperature, and has been previously assigned to a sub-chain rotation. [1] M.C. Scott, D.R. Stevens, J.R. Bochinski, L.I. Clarke, ACS Nano. DOI: 10.1021/nn800543j.

  20. Fabrication of self-assembled monolayers (SAMs) and inorganic micropattern on flexible polymer substrate.

    PubMed

    Xiang, Junhui; Zhu, Peixin; Masuda, Yoshitake; Koumoto, Kunihito

    2004-04-13

    By grafting (aminopropyl)triethoxysilane (APTES) as the buffer layer on poly(ethylene terephthalate) (PET) surface, the SAMs ofoctadecyltrichlorosilane (OTS), phenyltrichlorosilane (PTCS), vinyltrichlorosilane (VTCS), andp-tolyltrichlorosilane (TTCS) were fabricated on the flexible polymer substrate. The properties of SAMs were accurately controlled by adjusting the immersing time of substrates in the solutions and the concentration of the solutions. The SAMs acted as templates, and TiO2 micropattern was successfully deposited on OTS, TTCS, and PTCS SAMs. PMID:15875858

  1. Selective Plasma Deposition of Fluorocarbon Films on SAMs

    NASA Technical Reports Server (NTRS)

    Crain, Mark M., III; Walsh, Kevin M.; Cohn, Robert W.

    2006-01-01

    A dry plasma process has been demonstrated to be useful for the selective modification of self-assembled monolayers (SAMs) of alkanethiolates. These SAMs are used, during the fabrication of semiconductor electronic devices, as etch masks on gold layers that are destined to be patterned and incorporated into the devices. The selective modification involves the formation of fluorocarbon films that render the SAMs more effective in protecting the masked areas of the gold against etching by a potassium iodide (KI) solution. This modification can be utilized, not only in the fabrication of single electronic devices but also in the fabrication of integrated circuits, microelectromechanical systems, and circuit boards. In the steps that precede the dry plasma process, a silicon mold in the desired pattern is fabricated by standard photolithographic techniques. A stamp is then made by casting polydimethylsiloxane (commonly known as silicone rubber) in the mold. The stamp is coated with an alkanethiol solution, then the stamp is pressed on the gold layer of a device to be fabricated in order to deposit the alkanethiol to form an alkanethiolate SAM in the desired pattern (see figure). Next, the workpiece is exposed to a radio-frequency plasma generated from a mixture of CF4 and H2 gases. After this plasma treatment, the SAM is found to be modified, while the exposed areas of gold remain unchanged. This dry plasma process offers the potential for forming masks superior to those formed in a prior wet etching process. Among the advantages over the wet etching process are greater selectivity, fewer pin holes in the masks, and less nonuniformity of the masks. The fluorocarbon films formed in this way may also be useful as intermediate layers for subsequent fabrication steps and as dielectric layers to be incorporated into finished products.

  2. Isothiourea-Mediated Organocatalytic Michael Addition-Lactonization on a Surface: Modification of SAMs on Silicon Oxide Substrates.

    PubMed

    Chisholm, Ross; Parkin, John D; Smith, Andrew D; Hähner, Georg

    2016-04-01

    Tailoring the functionality of self-assembled monolayers (SAMs) can be achieved either by depositing prefunctionalized molecules with the appropriate terminal groups or by chemical modification of an existing SAM in situ. The latter approach is particularly advantageous to allow for diversity of surface functionalization from a single SAM and if the incorporation of bulky groups is desired. In the present study an organocatalytic isothiourea-mediated Michael addition-lactonization process analogous to a previously reported study in solution is presented. An achiral isothiourea, 3,4-dihydro-2H-pyrimido[2,1-b]benzothiazole (DHPB), promotes the intermolecular Michael addition-lactonization of a trifluoromethylenone terminated SAM and a variety of arylacetic acids affording C(6)-trifluoromethyldihydropyranones tethered to the surface. X-ray photoelectron spectroscopy, atomic force microscopy, contact angle, and ellipsometry analysis were conducted to confirm the presence of the substituted dihydropyranone. A model study of this approach was also performed in solution to probe the reaction diastereoselectivity as it cannot be measured directly on the surface. PMID:27015037

  3. 78 FR 3024 - Sam D. Hamilton Noxubee National Wildlife Refuge, MS; Intent To Prepare a Comprehensive...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-15

    ... Fish and Wildlife Service Sam D. Hamilton Noxubee National Wildlife Refuge, MS; Intent To Prepare a... conservation plan (CCP) and associated National Environmental Policy Act (NEPA) documents for Sam D. Hamilton... information to: Mr. Steve Reagan, Project Leader, Sam D. Hamilton Noxubee NWR, 2970 Bluff Lake...

  4. Chemoenzymatic synthesis and utilization of a SAM analog with an isomorphic nucleobase.

    PubMed

    Vranken, C; Fin, A; Tufar, P; Hofkens, J; Burkart, M D; Tor, Y

    2016-07-14

    SalL, an enzyme that catalyzes the synthesis of SAM from l-methionine and 5'-chloro-5'-deoxyoadenosine, is shown to accept 5'-chloro-5'-deoxythienoadenosine as a substrate and facilitate the synthesis of a synthetic SAM analog with an unnatural nucleobase. This synthetic cofactor is demonstrated to replace SAM in the DNA methylation reaction with M.TaqI. PMID:27270873

  5. 78 FR 30346 - Financial Investors Trust and Hanson McClain Strategic Advisors, Inc.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-22

    ... Conservative Fund, Pathway Advisors Growth & Income Fund, and Pathways Advisors Aggressive Growth Fund... relationship to an Unaffiliated Fund is covered by section 10(f) of the Act. 5. To further assure that...

  6. Mentoring Support and Relational Uncertainty in the Advisor-Advisee Relationship

    ERIC Educational Resources Information Center

    Mansson, Daniel H.; Myers, Scott A.

    2013-01-01

    We examine the extent to which career mentoring and psychosocial mentoring received from their advisors relates to advisee perceptions of advisor-advisee relational uncertainty. Doctoral students (N = 378) completed the "Academic Mentoring Behaviors Scale" (Schrodt, Cawyer, & Sanders, 2003), the "Mentoring and Communication…

  7. Industrial-Organizational and Human Factors Graduate Program Admission: Information for Undergraduate Advisors

    ERIC Educational Resources Information Center

    Shoenfelt, Elizabeth L.; Stone, Nancy J.; Kottke, Janet L.

    2015-01-01

    Many psychology departments do not have industrial-organizational (IO) or human factors (HF) faculty members. As such, potential IO and HF graduate students may miss career opportunities because faculty advisors are unfamiliar with the disciplines and their graduate programs. To assist advisors, this article highlights the content of IO and HF…

  8. 78 FR 14297 - President's Board of Advisors on Historically Black Colleges and Universities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-05

    ... President's Board of Advisors on Historically Black Colleges and Universities AGENCY: U.S. Department of... Black Colleges and Universities scheduled for March 6, 2013, and announced in the Federal Register on... Advisors on Historically Black Colleges and Universities, U.S. Department of Education, 400 Maryland...

  9. "What Haven't I Learned?" Learning Resulting from the Resident Advisor Role

    ERIC Educational Resources Information Center

    Benjamin, Mimi; Davis, Laura

    2016-01-01

    Employment can enhance learning for many college students, and on-campus jobs provide unique opportunities for this experience. The resident advisor position is one on-campus position offering the potential for learning experiences. This qualitative study highlights learning noted by 78 resident advisors at a large research institution during a…

  10. 76 FR 35054 - President's Council of Advisors on Science and Technology; Notice of Partially Closed Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-15

    ... From the Federal Register Online via the Government Publishing Office OFFICE OF SCIENCE AND TECHNOLOGY POLICY President's Council of Advisors on Science and Technology; Notice of Partially Closed... partially closed meeting of the President's Council of Advisors on Science and Technology (PCAST),...

  11. 78 FR 32281 - ProShares Advisors LLC, et al.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-29

    ... COMMISSION ProShares Advisors LLC, et al.; Notice of Application May 21, 2013. AGENCY: Securities and... exemption from sections 12(d)(1)(A) and (B) of the Act. Applicants: ProShares Advisors LLC (``ProShares''), ProShares Trust (the ``Trust''), and SEI Investments Distribution Co. (``SEI''). Summary...

  12. 78 FR 46388 - NGAM Advisors, L.P., et al.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-31

    ... COMMISSION NGAM Advisors, L.P., et al.; Notice of Application July 25, 2013. AGENCY: Securities and Exchange... Advisors, L.P. (the ``Adviser''), Natixis ETF Trust (the ``Trust'') and NGAM Distribution, L.P. Filing... mail. Hearing requests should be received by the Commission by 5:30 p.m. on August 19, 2013 and...

  13. Roles and Responsibilities of International Student Advisors and Counselors in the United States.

    ERIC Educational Resources Information Center

    Dalili, Farid

    The separate but complementary roles of the international student advisor and counselor are discussed, and foreign student personnel services are addressed. It is suggested that the increasing number of international students indicates a need for a full-time foreign student advisor. The job is complicated by the different cultures and different…

  14. A Preliminary Report of Advisor Perceptions of Advising and of a Profession

    ERIC Educational Resources Information Center

    Aiken-Wisniewski, Sharon A.; Johnson, Anna; Larson, Joshua; Barkemeyer, Jason

    2015-01-01

    Practicing advisors may not agree, know, or understand that advising does not meet the scholarly definition of a profession. Through a phenomenological study, members of NACADA: The Global Community for Academic Advising were invited to describe the position of academic advisor. The data gathered were used to address two research questions:…

  15. Gay-Straight Alliance Advisors: Negotiating Multiple Ecological Systems when Advocating for LGBTQ Youth

    ERIC Educational Resources Information Center

    Watson, Laurel B.; Varjas, Kris; Meyers, Joel; Graybill, Emily C.

    2010-01-01

    This qualitative study investigated the barriers and facilitators that advisors of gay-straight alliances encounter when advocating for LGBTQ (lesbian, gay, bisexual, transgender, and queer) youth within schools. Twenty-two advisors were interviewed, and data revealed that multiple ecological systems (e.g., sociocultural, school, and individual…

  16. Students Speak Out: Preliminary Qualitative Findings of a Mentor/Advisor Project.

    ERIC Educational Resources Information Center

    Welkowitz, Julie A.; Broer, Stephen M.; Topper, Karen; Thomas, Cassandra; Backus, Linda; Hamilton, Ruth

    This report explores the impact, through themes that emerged in interviews with 59 participating adolescents, of the Mentor/Advisor Program on students with or at risk of emotional disturbance. The Mentor/Advisor Program is implemented as part of the existing regular education high school curriculum and is designed to promote resiliency and school…

  17. 76 FR 18383 - Extension of Sunset Date for Attorney Advisor Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-04

    ... available on the date of publication in the Federal Register at http://www.gpoaccess.gov/fr/index.html... attorney advisors to conduct certain prehearing procedures. 72 FR 44763. We instituted this practice to... final rule. 73 FR 11349. Under this rule, attorney advisors may develop claims and, in appropriate...

  18. 78 FR 11865 - President's Board of Advisors on Historically Black Colleges and Universities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF EDUCATION President's Board of Advisors on Historically Black Colleges and Universities AGENCY: U.S. Department of Education, President's Board of Advisors on Historically Black Colleges and Universities (Board)....

  19. 77 FR 29621 - President's Board of Advisors on Historically Black Colleges and Universities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-18

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF EDUCATION President's Board of Advisors on Historically Black Colleges and Universities AGENCY: U.S. Department of Education, President's Board of Advisors on Historically Black Colleges and Universities (Board)....

  20. 75 FR 53279 - President's Board of Advisors on Historically Black Colleges and Universities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-31

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF EDUCATION President's Board of Advisors on Historically Black Colleges and Universities AGENCY: U.S. Department of Education, President's Board of Advisors on Historically Black Colleges and Universities (Board)....

  1. Assessment of the Student Peer Advisor Program: A Change in Roles.

    ERIC Educational Resources Information Center

    Phillips, Brad C.; Boren, Kelly J.

    Most academic advising conducted in the California Community Colleges is accomplished through a formal system administered by professional academic advisors/counselors. With recent cutbacks in funding, the amount of academic advisor time available to an increasing number of students is decreasing. In an effort to increase the availability of…

  2. Perceptions of Freedom and Commitment as Sources of Self-Efficacy among Pedagogical Advisors

    ERIC Educational Resources Information Center

    Kass, Efrat; Rajuan, Maureen

    2012-01-01

    The perceptions of the role of the pedagogical advisor are multiple and diverse. This amorphous situation places importance on the sense of self-efficacy among those filling this role. Examined in this study was what pedagogical advisors perceive as factors affecting their professional self-efficacy through in-depth interviews of 10 experienced…

  3. A Cross-Cultural Relationship between the Advisor and the Advisee: Dissertation Writing Supervision in Science.

    ERIC Educational Resources Information Center

    Dong, Yu Ren

    A study examined the supervisory relationship between a non-native English-speaking doctoral student and a native English-speaking advisor, particularly in science, focusing on factors leading to effective or ineffective supervision, advisee response to advisor guidance and assistance, and the roles played by the two participants. Subjects were…

  4. The Development of an International Student Advisor: A Grounded Theory Study

    ERIC Educational Resources Information Center

    Sparaco, Kathleen Keenan

    2012-01-01

    This qualitative study explored the professional experience of international student advisors. The statement of problem for this research was that the professional role of international student advisors has not been clearly defined or understood within U.S. higher education. The research questions asked (1) what encompassed the lived experience of…

  5. 78 FR 69155 - Altegris Advisors, L.L.C., et al.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-18

    ... COMMISSION Altegris Advisors, L.L.C., et al.; Notice of Application November 12, 2013. AGENCY: Securities and...: Altegris Advisors, L.L.C. (the ``Adviser'') and Northern Lights Fund Trust (the ``Trust''), on behalf of... section 12(d)(l)(A)(i) of the Act, the Board of the Unaffiliated Investment Company, including a...

  6. Student Organization Advisor Motives to Volunteer at Four-Year Institutions of Higher Education

    ERIC Educational Resources Information Center

    Meyer, Robert A.; Kroth, Michael

    2010-01-01

    Many higher education institutions require student organization advisors to be employees. Such requirements can be interpreted as barriers to students and can limit the number and types of groups found on college campuses. Using quantitative analysis, this study investigates the motivation of student organization advisors at six public…

  7. 77 FR 13296 - Subcommittee Meeting of the Board of Advisors to the President, Naval Postgraduate School

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-06

    ... Department of the Navy Subcommittee Meeting of the Board of Advisors to the President, Naval Postgraduate... Advisors to the Presidents of the Naval Postgraduate School and the Naval War College). This meeting will... meeting will be held at the Naval Postgraduate School, Herrmann Hall, 1 University Circle, Didactic...

  8. 78 FR 15057 - The Advisors' Inner Circle Fund, et al.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-08

    ... Capital Advisors, LLC (``PNC''), Frost Investment Advisors, LLC (``Frost''), GRT Capital Partners, L.L.C... series of AIC.\\4\\ Frost, a Delaware limited liability company and a wholly owned non- banking subsidiary of Frost Bank, is registered as an investment adviser under the Advisers Act and currently serves...

  9. The Advisor and Instructor as a Dynamic Duo: Academic Motivation and Basic Psychological Needs

    ERIC Educational Resources Information Center

    Burt, Tracie D.; Young-Jones, Adena D.; Yadon, Carly A.; Carr, Michael T.

    2013-01-01

    Students learn in and out of a formal classroom, and instructors and academic advisors play key roles in academic motivation and learning. Therefore, through the lens of self-determination theory, we examined the ways perceived support from instructors and advisors relates to satisfaction of college students' basic psychological needs.…

  10. 12 CFR 250.406 - Serving member bank and investment advisor with mutual fund affiliation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 4 2013-01-01 2013-01-01 false Serving member bank and investment advisor with... Interpretations of Section 32 of the Glass-Steagall Act § 250.406 Serving member bank and investment advisor with... requested with respect to service as vice president of a corporation engaged in supplying investment...

  11. 12 CFR 250.406 - Serving member bank and investment advisor with mutual fund affiliation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 4 2012-01-01 2012-01-01 false Serving member bank and investment advisor with... Interpretations of Section 32 of the Glass-Steagall Act § 250.406 Serving member bank and investment advisor with... requested with respect to service as vice president of a corporation engaged in supplying investment...

  12. 12 CFR 250.406 - Serving member bank and investment advisor with mutual fund affiliation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 3 2011-01-01 2011-01-01 false Serving member bank and investment advisor with... Section 32 of the Glass-Steagall Act § 250.406 Serving member bank and investment advisor with mutual fund... respect to service as vice president of a corporation engaged in supplying investment advice...

  13. 12 CFR 250.406 - Serving member bank and investment advisor with mutual fund affiliation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 4 2014-01-01 2014-01-01 false Serving member bank and investment advisor with... Interpretations of Section 32 of the Glass-Steagall Act § 250.406 Serving member bank and investment advisor with... requested with respect to service as vice president of a corporation engaged in supplying investment...

  14. Using Mentoring Enactment Theory to Explore the Doctoral Student-Advisor Mentoring Relationship

    ERIC Educational Resources Information Center

    Mansson, Daniel H.; Myers, Scott A.

    2012-01-01

    The purposes of this study were (a) to develop a new measure to assess doctoral advisees' use of relational maintenance behaviors with their advisors, and (b) to examine both advisees' (n = 636) and advisors' (n = 141) perceptions of their mentoring relationship using mentoring enactment theory (MET; Kalbfleisch, 2002). The results of…

  15. 17 CFR 4.27 - Additional reporting by advisors of certain large commodity pools.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... section 4.10(f); (4) Net asset value or NAV has the same meaning as net asset value as defined in section... advisors of certain large commodity pools. 4.27 Section 4.27 Commodity and Securities Exchanges COMMODITY... Operators § 4.27 Additional reporting by advisors of certain large commodity pools. (a) General...

  16. 17 CFR 4.27 - Additional reporting by advisors of certain large commodity pools.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... section 4.10(f); (4) Net asset value or NAV has the same meaning as net asset value as defined in section... advisors of certain large commodity pools. 4.27 Section 4.27 Commodity and Securities Exchanges COMMODITY... Operators § 4.27 Additional reporting by advisors of certain large commodity pools. (a) General...

  17. 76 FR 66096 - RiverPark Advisors, LLC, et al.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-25

    ... COMMISSION RiverPark Advisors, LLC, et al.; Notice of Application October 19, 2011. AGENCY: Securities and... exemption from sections 12(d)(1)(A) and (B) of the Act. Applicants: RiverPark Advisors, LLC (``RiverPark''), RiverPark Funds Trust (the ``Trust'') and ALPS Distributors, Inc. (the ``Distributor''). Summary...

  18. Working with Youth on LGBT Issues: Why Gay-Straight Alliance Advisors Become Involved

    ERIC Educational Resources Information Center

    Valenti, Maria; Campbell, Rebecca

    2009-01-01

    This phenomenological qualitative study explores motivation for citizen participation in a local context by exploring the experiences of Gay-Straight Alliance (GSA) club advisors from public high schools. Fourteen advisors from one midwestern state were randomly recruited for participation. Inductive and deductive qualitative analyses elucidated…

  19. 78 FR 77175 - Catalyst Capital Advisors LLC and Mutual Fund Series Trust; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-20

    ... COMMISSION Catalyst Capital Advisors LLC and Mutual Fund Series Trust; Notice of Application December 16... agreements without shareholder approval. APPLICANTS: Catalyst Capital Advisors LLC (``CCA'' or the ``Adviser'') and Mutual Fund Series Trust (formerly Catalyst Funds) (the ``Trust''). DATES: Filing Dates:...

  20. 75 FR 9006 - President's Council of Advisors on Science and Technology; Notice of Meeting: Partially Closed...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-26

    ... areas where understanding of science, technology, and innovation is key to strengthening our economy and... TECHNOLOGY POLICY President's Council of Advisors on Science and Technology; Notice of Meeting: Partially Closed Meeting of the President's Council of Advisors on Science and Technology ACTION: Public...

  1. 40 CFR 35.4190 - How does my group identify a qualified technical advisor?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... example, toxicology). (c) Your group should select a technical advisor who has experience working on... 40 Protection of Environment 1 2010-07-01 2010-07-01 false How does my group identify a qualified... Technical Advisor Or Other Contractor with Tag Funds § 35.4190 How does my group identify a...

  2. Academic Advisee Motives for Pursuing Out-of-Class Communication with the Faculty Academic Advisor

    ERIC Educational Resources Information Center

    Leach, Rebecca B.; Wang, Tiffany R.

    2015-01-01

    This study examined advisee communication motives for engaging in out-of-class communication (OCC) with the faculty academic advisor. Undergraduate students (n = 21) were interviewed about their motives for engaging in OCC with their faculty academic advisors. In a thematic analysis, six motives emerged for engaging in OCC with faculty academic…

  3. Standards Advisor-Advanced Information Technology for Advanced Information Delivery

    NASA Technical Reports Server (NTRS)

    Hawker, J. Scott

    2003-01-01

    Developers of space systems must deal with an increasing amount of information in responding to extensive requirements and standards from numerous sources. Accessing these requirements and standards, understanding them, comparing them, negotiating them and responding to them is often an overwhelming task. There are resources to aid the space systems developer, such as lessons learned and best practices. Again, though, accessing, understanding, and using this information is often more difficult than helpful. This results in space systems that: 1. Do not meet all their requirements. 2. Do not incorporate prior engineering experience. 3. Cost more to develop. 4. Take longer to develop. The NASA Technical Standards Program (NTSP) web site at http://standards.nasa.gov has made significant improvements in making standards, lessons learned, and related material available to space systems developers agency-wide. The Standards Advisor was conceived to take the next steps beyond the current product, continuing to apply evolving information technology that continues to improve information delivery to space systems developers. This report describes the features of the Standards Advisor and suggests a technical approach to its development.

  4. [Preliminary study on autoregulation of samR involved in development and differentiation of Streptomyces ansochromogenes].

    PubMed

    Yang, Yan-ling; Yang, Hai-hua; Tan, Hua-rong

    2005-02-01

    The previous result showed that samR plays an important role in the development progress of Streptomyces ansochromogenes. It was reported that the differentiation progress of S. ansochromogenes was accelerated by a recombinant plasmid containing an extra copy of samR gene. However, the differentiation progress of S. ansochromogenes was not further accelerated by a multicopy plasmid containing samR gene. Electrophoresis mobility shift assay (EMSA) demonstrated that SamR binds to its own promoter region specifically. All these results hint that samR is an autoregulatory gene in Streptomyces ansochromogenes. PMID:15847153

  5. Summary Status of the Space Acceleration Measurement System (SAMS), September 1993

    NASA Technical Reports Server (NTRS)

    DeLombard, Richard

    1994-01-01

    The Space Acceleration Measurement System (SAMS) was developed to measure the microgravity acceleration environment to which NASA science payloads are exposed during microgravity science missions on the shuttle. Six flight units have been fabricated to date. The inaugural flight of a SAMS unit was on STS-40 in June 1991 as part of the First Spacelab Life Sciences mission. Since that time, SAMS has flown on six additional missions and gathered eighteen gigabytes of data representing sixty-eight days of microgravity environment. The SAMS units have been flown in the shuttle middeck and cargo bay, in the Spacelab module, and in the Spacehab module. This paper summarizes the missions and experiments which SAMS has supported. The quantity of data and the utilization of the SAMS data is described. Future activities are briefly described for the SAMS project and the Microgravity Measurement and Analysis project (MMAP) to support science experiments and scientists with microgravity environment measurement and analysis.

  6. First use of SAM onboard calibration gas cell

    NASA Astrophysics Data System (ADS)

    Malespin, C.; Trainer, M. G.; Manning, H. L.; Franz, H. B.; Conrad, P. G.; Raaen, E.; Webster, C. R.; Flesch, G.; Eigenbrode, J. L.; Wong, M. H.; Mahaffy, P. R.

    2015-12-01

    The Sample Analysis at Mars (SAM) instrument (Mahaffy et al 2012) suite on Curiosity completed its first measurement of the onboard calibration gas cell on MSL Mission Sol 1042. The cell consists of a gas mixture of four primary gases, along with trace fluorinated hydrocarbon high mass calibrants. The mix is comprised of approximately 25% CO2, N2, Xe and Ar, where the 129Xe has been given a three times enrichment relative to terrestrial xenon in order to distinguish it isotopically from Martian atmospheric Xe. Analysis of the calibration cell is intended to identify changes in instrument performance between pre-launch calibrations and operations on Mars, for any of the three main subsystems in SAM: the Quadrupole Mass Spectrometer (QMS), Tunable Laser Spectrometer (TLS), and Gas Chromatograph (GC). Here we present the experimental approach, results, and implications for instrument performance after almost three years of measurements on Mars.

  7. AstroNomical Information System at CeSAM

    NASA Astrophysics Data System (ADS)

    Gimenez, S.; Moreau, C.; Agneray, F.; Roehlly, Y.

    2014-05-01

    AstroNomical Information System (ANIS), developed by the Centre de donnéeS Astrophysique de Marseille (CeSAM), is a generic tool aimed at facilitating and homogenizing the implementation of astronomical data of various kinds and in dedicated Information Systems. ANIS provides high level services like: search, extract and display imaging and spectroscopic data using a combination of criteria, an object list, a sql query module or a cone search interfaces, as well as download of catalogs and complete datasets. With ANIS, the CeSAM offers web access to VO compliant Information Systems for different projects VVDS, HeDAM, ExoDat, HST-COSMOS, etc.), including ancillary data that are cross-matched before ingestion.

  8. 77 FR 60732 - PACE Select Advisors Trust and UBS Global Asset Management (Americas) Inc.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-04

    ... COMMISSION PACE Select Advisors Trust and UBS Global Asset Management (Americas) Inc.; Notice of Application... requirements. The requested order would supersede two prior orders.\\1\\ \\1\\ PaineWebber PACE Select Advisors...) and 21666 (Jan. 11, 1996) (order). Applicants: PACE Select Advisors Trust (the ``Trust'') and...

  9. Advisor-Advisee Three: Graduate Students' Perceptions of Verbal Aggression, Credibility, and Conflict Styles in the Advising Relationship

    ERIC Educational Resources Information Center

    Punyanunt-Carter, Narissra M.; Wrench, Jason S.

    2008-01-01

    The current study looked at the relation between advisee perceptions of advisor's verbal aggression, credibility, and conflict styles. Participants were 153 graduate students who reported their perceptions concerning their advisor. First, the study found that advisee perceptions of their advisor's credibility (competence, caring/goodwill, &…

  10. TNF-α produced by SEC2 mutant (SAM-3)-activated human T cells induces apoptosis of HepG2 cells.

    PubMed

    Zhang, Guojun; Xu, Mingkai; Song, Yubo; Su, Zhencheng; Zhang, Huiwen; Zhang, Chenggang

    2016-03-01

    Staphylococcal enterotoxins C2 (SEC2) is a classical model of superantigens (SAg), which has the powerful ability to activate T cells as well as induce massive cytokine production. This property makes SEC2 and its mutants well concerned as a potential new immune-regulatory agent for cancer therapy. We previously constructed a SEC2 mutant named SAM-3, which had prominently antitumor activity in BALB/c mice model. But, the underlying molecular mechanism for stimulation of human peripheral blood mononuclear cells (PBMCs) and antitumor effect on human tumor cells induced by SAM-3 is not clear. Here, we showed that SAM-3 could activate human TCR Vβ 12, 13A, 14, 15, 17, and 20 CD8(+) subgroup T cells, which secreted the cytokines IL-2, IFN-γ, and TNF-α, and exhibit stimulation activity in a dose-dependent manner. TNF-α secreted from activated T cells could induce apoptosis and G1-phase arrest and lead to the antitumor effect in HepG2 cells. Meanwhile, SAM-3 upregulated the expression of tumor necrosis factor receptor 1 (TNFR1) mRNA and activity of caspase-3 and caspase-8. We also found that the antitumor activity and activity of caspase-3 and caspase-8 were decreased when the neutralizing TNF-α monoclonal antibody presented. These data suggest that TNF-α secreted by SAM-3-activated T cells is an important factor in inducing apoptosis in HepG2 cells. PMID:26536876

  11. Thiostrepton tryptophan methyltransferase expands the chemistry of radical SAM enzymes.

    PubMed

    Pierre, Stéphane; Guillot, Alain; Benjdia, Alhosna; Sandström, Corine; Langella, Philippe; Berteau, Olivier

    2012-12-01

    Methylation is among the most widespread chemical modifications encountered in biomolecules and has a pivotal role in many major biological processes. In the biosynthetic pathway of the antibiotic thiostrepton A, we identified what is to our knowledge the first tryptophan methyltransferase. We show that it uses unprecedented chemistry to methylate inactivated sp(2)-hybridized carbon atoms, despite being predicted to be a radical SAM enzyme. PMID:23064318

  12. GLAO4ELT: trade study and SAM experience

    NASA Astrophysics Data System (ADS)

    Tokovinin, A.

    2011-09-01

    It is expected that a large fraction of wave-front distortions at ELTs will be anisoplanatic, being produced jointly by near-ground and dome turbulence, wind buffeting, flexures, and vibrations. We compare various strategies for sensing this "ground" component using (i) several sodium LGSs, (ii) one or several Rayleigh LGSs and (ii) NGSs in a wide field. The experience gained so far with the RLGS GLAO system SAM at 4-m telescope will be presented and discussed.

  13. Mechanistic Enzymology of the Radical SAM Enzyme DesII

    PubMed Central

    2016-01-01

    DesII is a member of the radical SAM family of enzymes that catalyzes radical-mediated transformations of TDP-4-amino-4,6-didexoy-D-glucose as well as other sugar nucleotide diphosphates. Like nearly all radical SAM enzymes, the reactions begin with the reductive homolysis of SAM to produce a 5′-deoxyadenosyl radical which is followed by regiospecific hydrogen atom abstraction from the substrate. What happens next, however, depends on the nature of the substrate radical so produced. In the case of the biosynthetically relevant substrate, a radical-mediated deamination ensues; however, when this amino group is replaced with a hydroxyl, one instead observes dehydrogenation. The factors that govern the fate of the initially generated substrate radical as well as the mechanistic details underlying these transformations have been a key focus of research into the chemistry of DesII. This review will discuss recent discoveries pertaining to the enzymology of DesII, how it may relate to understanding other radical-mediated lyases and dehydrogenases and the working hypotheses currently being investigated regarding the mechanism of DesII catalysis.

  14. CE-SAM: a conversational interface for ISR mission support

    NASA Astrophysics Data System (ADS)

    Pizzocaro, Diego; Parizas, Christos; Preece, Alun; Braines, Dave; Mott, David; Bakdash, Jonathan Z.

    2013-05-01

    There is considerable interest in natural language conversational interfaces. These allow for complex user interactions with systems, such as fulfilling information requirements in dynamic environments, without requiring extensive training or a technical background (e.g. in formal query languages or schemas). To leverage the advantages of conversational interactions we propose CE-SAM (Controlled English Sensor Assignment to Missions), a system that guides users through refining and satisfying their information needs in the context of Intelligence, Surveillance, and Reconnaissance (ISR) operations. The rapidly-increasing availability of sensing assets and other information sources poses substantial challenges to effective ISR resource management. In a coalition context, the problem is even more complex, because assets may be "owned" by different partners. We show how CE-SAM allows a user to refine and relate their ISR information needs to pre-existing concepts in an ISR knowledge base, via conversational interaction implemented on a tablet device. The knowledge base is represented using Controlled English (CE) - a form of controlled natural language that is both human-readable and machine processable (i.e. can be used to implement automated reasoning). Users interact with the CE-SAM conversational interface using natural language, which the system converts to CE for feeding-back to the user for confirmation (e.g. to reduce misunderstanding). We show that this process not only allows users to access the assets that can support their mission needs, but also assists them in extending the CE knowledge base with new concepts.

  15. SAMS Acceleration Measurements on Mir (NASA Increment 4)

    NASA Technical Reports Server (NTRS)

    DeLombard, Richard

    1998-01-01

    During NASA Increment 4 (January to May 1997), about 5 gigabytes of acceleration data were collected by the Space Acceleration Measurements System (SAMS) onboard the Russian Space Station, Mir. The data were recorded on 28 optical disks which were returned to Earth on STS-84. During this increment, SAMS data were collected in the Priroda module to support the Mir Structural Dynamics Experiment (MiSDE), the Binary Colloidal Alloy Tests (BCAT), Angular Liquid Bridge (ALB), Candle Flames in Microgravity (CFM), Diffusion Controlled Apparatus Module (DCAM), Enhanced Dynamic Load Sensors (EDLS), Forced Flow Flame Spreading Test (FFFr), Liquid Metal Diffusion (LMD), Protein Crystal Growth in Dewar (PCG/Dewar), Queen's University Experiments in Liquid Diffusion (QUELD), and Technical Evaluation of MIM (TEM). This report points out some of the salient features of the microgravity environment to which these experiments were exposed. Also documented are mission events of interest such as the docked phase of STS-84 operations, a Progress engine bum, Soyuz vehicle docking and undocking, and Progress vehicle docking. This report presents an overview of the SAMS acceleration measurements recorded by 10 Hz and 100 Hz sensor heads. The analyses included herein complement those presented in previous summary reports prepared by the Principal Investigator Microgravity Services (PIMS) group.

  16. In situ analysis of martian regolith with the SAM experiment during the first mars year of the MSL mission: Identification of organic molecules by gas chromatography from laboratory measurements

    NASA Astrophysics Data System (ADS)

    Millan, M.; Szopa, C.; Buch, A.; Coll, P.; Glavin, D. P.; Freissinet, C.; Navarro-Gonzalez, R.; François, P.; Coscia, D.; Bonnet, J. Y.; Teinturier, S.; Cabane, M.; Mahaffy, P. R.

    2016-09-01

    The Sample Analysis at Mars (SAM) instrument onboard the Curiosity rover, is specifically designed for in situ molecular and isotopic analyses of martian surface materials and atmosphere. It contributes to the Mars Science Laboratory (MSL) missions primary scientific goal to characterize the potential past, present or future habitability of Mars. In all of the analyses of solid samples delivered to SAM so far, chlorinated organic compounds have been detected above instrument background levels and identified by gas chromatography coupled to mass spectrometry (GC-MS) (Freissinet et al., 2015; Glavin et al., 2013). While some of these may originate from reactions between oxychlorines and terrestrial organic carbon present in the instrument background (Glavin et al., 2013), others have been demonstrated to originate from indigenous organic carbon present in samples (Freissinet et al., 2015). We present here laboratory calibrations that focused on the analyses performed with the MXT-CLP GC column (SAM GC-5 channel) used for nearly all of the GC-MS analyses of the martian soil samples carried out with SAM to date. Complementary to the mass spectrometric data, gas chromatography allows us to separate and identify the species analyzable in a nominal SAM-GC run time of about 21 min. To characterize the analytical capabilities of this channel within the SAM Flight Model (FM) operating conditions on Mars, and their implications on the detection of organic matter, it is required to perform laboratory experimental tests and calibrations on spare model components. This work assesses the SAM flight GC-5 column efficiency, confirms the identification of the molecules based on their retention time, and enables a better understanding of the behavior of the SAM injection trap (IT) and its release of organic molecules. This work will enable further optimization of the SAM-GC runs for additional samples to be analyzed during the MSL mission.

  17. The SAMS: Smartphone Addiction Management System and verification.

    PubMed

    Lee, Heyoung; Ahn, Heejune; Choi, Samwook; Choi, Wanbok

    2014-01-01

    While the popularity of smartphones has given enormous convenience to our lives, their pathological use has created a new mental health concern among the community. Hence, intensive research is being conducted on the etiology and treatment of the condition. However, the traditional clinical approach based surveys and interviews has serious limitations: health professionals cannot perform continual assessment and intervention for the affected group and the subjectivity of assessment is questionable. To cope with these limitations, a comprehensive ICT (Information and Communications Technology) system called SAMS (Smartphone Addiction Management System) is developed for objective assessment and intervention. The SAMS system consists of an Android smartphone application and a web application server. The SAMS client monitors the user's application usage together with GPS location and Internet access location, and transmits the data to the SAMS server. The SAMS server stores the usage data and performs key statistical data analysis and usage intervention according to the clinicians' decision. To verify the reliability and efficacy of the developed system, a comparison study with survey-based screening with the K-SAS (Korean Smartphone Addiction Scale) as well as self-field trials is performed. The comparison study is done using usage data from 14 users who are 19 to 50 year old adults that left at least 1 week usage logs and completed the survey questionnaires. The field trial fully verified the accuracy of the time, location, and Internet access information in the usage measurement and the reliability of the system operation over more than 2 weeks. The comparison study showed that daily use count has a strong correlation with K-SAS scores, whereas daily use times do not strongly correlate for potentially addicted users. The correlation coefficients of count and times with total K-SAS score are CC = 0.62 and CC =0.07, respectively, and the t-test analysis for the

  18. The Improvement of SAM Accumulation by Integrating the Endogenous Methionine Adenosyltransferase Gene SAM2 in Genome of the Industrial Saccharomyces cerevisiae Strain.

    PubMed

    Zhao, Weijun; Shi, Feng; Hang, Baojian; Huang, Lei; Cai, Jin; Xu, Zhinan

    2016-03-01

    S-Adenosyl-L-methionine (SAM) plays important roles in trans-methylation, trans-sulfuration, and polyamine synthesis in all living cells, and it is also an effective cure for liver disease, depressive syndromes, and osteoarthritis. The increased demands of SAM in pharmaceuticals industry have aroused lots of attempts to improve its production. In this study, a multiple-copy integrative plasmid pYMIKP-SAM2 was introduced into the chromosome of wild-type Saccharomyces cerevisiae strain ZJU001 to construct the recombined strain R1-ZJU001. Further studies showed that the recombinant yeast exhibited higher enzymatic activity of methionine adenosyltransferase and improved its SAM biosynthesis. With a three-phase fed-batch strategy in 15-liter bench-top fermentor, 8.81 g/L SAM was achieved after 52 h cultivation of R1-ZJU001, about 27.1 % increase over its parent strain ZJU001, whereas the SAM content was also improved from 64.6 mg/g DCW to 91.0 mg/g DCW. Our results shall provide insights into the metabolic engineering of SAM pathway in yeast for improved productivity of SAM and subsequent industrial applications. PMID:26728652

  19. Science Advisor and Applied Physicist: Joseph Henry Serves His Country

    NASA Astrophysics Data System (ADS)

    Rothenberg, Marc

    1997-04-01

    When Joseph Henry accepted the postion of secretary of the Smithsonian in 1846, his career changed radically. Although he never ceased thinking of himself as a research scientist and educator, thereafter his chief roles were those of science administrator and advisor to both the executive and legislative branches of the federal government for both science and technology. His effectiveness as an advocate became more important than his skill as an experimental physicist. Even when he entered the laboratory, his role had changed. No longer was he concerned with basic research. As a member of various government boards and committees, Henry spent the last three decades of his life concerned with the application of fundamental knowledge for the improvement of the human condition. This paper will discuss Henry's service on behalf of his country.

  20. Multi-Center Traffic Management Advisor Operational Field Test Results

    NASA Technical Reports Server (NTRS)

    Farley, Todd; Landry, Steven J.; Hoang, Ty; Nickelson, Monicarol; Levin, Kerry M.; Rowe, Dennis W.

    2005-01-01

    The Multi-Center Traffic Management Advisor (McTMA) is a research prototype system which seeks to bring time-based metering into the mainstream of air traffic control (ATC) operations. Time-based metering is an efficient alternative to traditional air traffic management techniques such as distance-based spacing (miles-in-trail spacing) and managed arrival reservoirs (airborne holding). While time-based metering has demonstrated significant benefit in terms of arrival throughput and arrival delay, its use to date has been limited to arrival operations at just nine airports nationally. Wide-scale adoption of time-based metering has been hampered, in part, by the limited scalability of metering automation. In order to realize the full spectrum of efficiency benefits possible with time-based metering, a much more modular, scalable time-based metering capability is required. With its distributed metering architecture, multi-center TMA offers such a capability.

  1. WinMod: An expert advisor for investment casting

    SciTech Connect

    Bivens, H.P.; Williamson, G.A. Jr.; Luger, G.F.; Erdmann, R.G.; Maguire, M.C.; Baldwin, M.D.; Anderson, D.J.

    1998-04-01

    Investment casting is an important method for fabricating a variety of high quality components in mechanical systems. Cast components, unfortunately, have a large design and gate/runner build time associated with their fabrication. In addition, casting engineers often require many years of actual experience in order to consistently pour high quality castings. Since 1989, Sandia National Laboratories has been investigating casting technology and software that will reduce the time overhead involved in producing quality casts. Several companies in the casting industry have teamed up with Sandia to form the FASTCAST Consortium. One result of this research and the formation of the FASTCAST consortium is the creation of the WinMod software, an expert casting advisor that supports the decision making process of the casting engineer through visualization and advice to help eliminate possible casting defects.

  2. [Senescence-accelerated mouse (SAM): with special reference to age-associated pathologies and their modulation].

    PubMed

    Takeda, T

    1996-07-01

    The senescence-accelerated mouse (SAM) has been under development by our research team at Kyoto University since 1970 through selective inbreeding of the AKR/J strain of mice donated by the Jackson Laboratory in 1968, based on the data of the grading score of senescence, life span, and pathologic phenotypes. At present, there are 12 lines of SAM; the 9 senescence-prone inbred strains (SAMP) include SAMP1, SAMP2, SAMP3, SAMP6, SAMP7, SAMP8, SAMP9, SAMP10 and SAMP11, and the 3 senescence-resistant inbred strains (SAMR) SAMR1, SANR4 and SAMR5. Data from survival curves, the Gompertzian function and the grading score of senescence, together with growth patterns of body weight of these SAMP and SAMR mice revealed that the characteristic feature of aging common to all SAMP mice is "accelerated senescence": early onset and irreversible advance of senescence manifested by several signs and gross lesions such as the loss of normal behavior, various skin lesions, increased lordokyphosis, etc., after a period of normal development. Routine postmortem examinations and the pathobiological features revealed by systematically designed studies have shown several pathologic phenotypes, which are often characteristic enough to differentiate among the various SAM strains: senile amyloidosis in SAMP1, -P2, -P7, -P9, -P10 and -P11, secondary amyloidosis in SAMP2 and -P6, contracted kidney in SAMP1, -P2, -P10, -P11, immunoblastic lymphoma in SAMR1 and -R4, histiocytic sarcoma in SAMR1 and -R4, ovarian cysts in SAMR1, impaired immune response in SAMP1, -P2 and -P8, hyperinflation of the lungs in SAMP1, hearing impairment in SAMP1, degenerative temporomandibular joint disease in SAMP3, senile osteoporosis in SAMP6, deficits in learning and memory in SAMP8 and -P10, emotional disorders in SAMP8 and -P10, cataracts in SAMP9, and brain atrophy in SAMP10. These are all age-associated pathologies, the incidence and severity of which increase with advancing age. The SAM model in which these

  3. Unliganded EphA3 dimerization promoted by the SAM domain

    PubMed Central

    Singh, Deo R.; Cao, QingQing; King, Christopher; Salotto, Matt; Ahmed, Fozia; Zhou, Xiang Yang; Pasquale, Elena B.; Hristova, Kalina

    2015-01-01

    The erythropoietin-producing hepatocellular carcinoma A3 (EphA3) receptor tyrosine kinase (RTK) regulates morphogenesis during development and is overexpressed and mutated in a variety of cancers. EphA3 activation is believed to follow a ‘seeding mechanism’ model, in which ligand binding to the monomeric receptor acts as a trigger for signal-productive receptor clustering. We study EphA3 lateral interactions on the surface of live cells and we demonstrate that EphA3 forms dimers in the absence of ligand binding. We further show that these dimers are stabilized by interactions involving the EphA3 sterile α-motif (SAM) domain. The discovery of unliganded EphA3 dimers challenges the current understanding of the chain of EphA3 activation events and suggests that EphA3 may follow the ‘pre-formed dimer’ model of activation known to be relevant for other receptor tyrosine kinases. The present work also establishes a new role for the SAM domain in promoting Eph receptor lateral interactions and signalling on the cell surface. PMID:26232493

  4. An intelligent, knowledge-based multiple criteria decision making advisor for systems design

    NASA Astrophysics Data System (ADS)

    Li, Yongchang

    In systems engineering, design and operation of systems are two main problems which always attract researcher's attentions. The accomplishment of activities in these problems often requires proper decisions to be made so that the desired goal can be achieved, thus, decision making needs to be carefully fulfilled in the design and operation of systems. Design is a decision making process which permeates through out the design process, and is at the core of all design activities. In modern aircraft design, more and more attention is paid to the conceptual and preliminary design phases so as to increase the odds of choosing a design that will ultimately be successful at the completion of the design process, therefore, decisions made during these early design stages play a critical role in determining the success of a design. Since aerospace systems are complex systems with interacting disciplines and technologies, the Decision Makers (DMs) dealing with such design problems are involved in balancing the multiple, potentially conflicting attributes/criteria, transforming a large amount of customer supplied guidelines into a solidly defined set of requirement definitions. Thus, one could state with confidence that modern aerospace system design is a Multiple Criteria Decision Making (MCDM) process. A variety of existing decision making methods are available to deal with this type of decision problems. The selection of the most appropriate decision making method is of particular importance since inappropriate decision methods are likely causes of misleading engineering design decisions. With no sufficient knowledge about each of the methods, it is usually difficult for the DMs to find an appropriate analytical model capable of solving their problems. In addition, with the complexity of the decision problem and the demand for more capable methods increasing, new decision making methods are emerging with time. These various methods exacerbate the difficulty of the selection

  5. Systematic biochemical characterization of the SAM domains in Eph receptor family from Mus Musculus.

    PubMed

    Wang, Yue; Li, Qingxia; Zheng, Yunhua; Li, Gang; Liu, Wei

    2016-05-13

    The Eph receptor family is the largest subfamily of receptor tyrosine kinases and well-known for their pivotal roles in axon guidance, synaptogenesis, artery/venous differentiation and tumorigenesis, etc. Activation of the Eph receptor needs multimerization of the receptors. The intracellular C-terminal SAM domain of Eph receptor was reported to mediate self-association of Eph receptors via the homo SAM-SAM interaction. In this study, we systematically expressed and purified the SAM domain proteins of all fourteen Eph receptors of Mus musculus in Escherichia coli. The FPLC (fast protein liquid chromatography) results showed the recombinant SAM domains were highly homogeneous. Using CD (circular dichroism) spectrometry, we found that the secondary structure of all the SAM domains was typically alpha helical folded and remarkably similar. The thermo-stability tests showed that they were quite stable in solution. SEC-MALS (size exclusion chromatography coupled with multiple angle light scattering) results illustrated 200 μM Eph SAM domains behaved as good monomers in the size-exclusion chromatography. More importantly, DLS (dynamic light scattering) results revealed the overwhelming majority of SAM domains was not multimerized in solution either at 200 μM or 2000 μM protein concentration, which indicating the SAM domain alone was not sufficient to mediate the polymerization of Eph receptor. In summary, our studies provided the systematic biochemical characterizations of the Eph receptor SAM domains and implied their roles in Eph receptor mediated signaling pathways. PMID:27086853

  6. NREL's System Advisor Model Simplifies Complex Energy Analysis (Fact Sheet)

    SciTech Connect

    Not Available

    2011-10-01

    The energy market is diversifying. In addition to traditional power sources, decision makers can choose among solar, wind, and geothermal technologies as well. Each of these technologies has complex performance characteristics and economics that vary with location and other project specifics, making it difficult to analyze the viability of such projects. But that analysis is easier now, thanks to the National Renewable Energy Laboratory (NREL).

  7. Benchmark Simulations of the Thermal-Hydraulic Responses during EBR-II Inherent Safety Tests using SAM

    SciTech Connect

    Hu, Rui; Sumner, Tyler S.

    2016-01-01

    An advanced system analysis tool SAM is being developed for fast-running, improved-fidelity, and wholeplant transient analyses at Argonne National Laboratory under DOE-NE’s Nuclear Energy Advanced Modeling and Simulation (NEAMS) program. As an important part of code development, companion validation activities are being conducted to ensure the performance and validity of the SAM code. This paper presents the benchmark simulations of two EBR-II tests, SHRT-45R and BOP- 302R, whose data are available through the support of DOE-NE’s Advanced Reactor Technology (ART) program. The code predictions of major primary coolant system parameter are compared with the test results. Additionally, the SAS4A/SASSYS-1 code simulation results are also included for a code-to-code comparison.

  8. Detection of Nitric Oxide by the Sample Analysis at Mars (SAM) Instrument Implications for the Presence of Nitrates

    NASA Technical Reports Server (NTRS)

    Navarro-Gonzalez, R.; Stern, J.; Freissinet, C.; Franz, H. B.; Eigenbrode, J. L..; McKay, C. P.; Coll, P.; Sutter, B.; Archer, D.; McAdam, A.; Cabane, M.; Ming, D. W.; Glavin, D.; Leshin, L.; Wong, M.; Atreya, S.; Wray, J. J.; Steele, A.; Buch, A.; Prats, B. D.; Szopa, C.; Coscia, D.; Teinturier, S.; Conrad, P.; Owen, T. C.; Mahaffy, P.; Grotzinger, J. P.

    2014-01-01

    One of the main goals of the Mars Science Laboratory is to determine whether the planet ever had environmental conditions able to support microbial life. Nitrogen is a fundamental element for life, and is present in structural (e.g., proteins), catalytic (e.g., enzymes and ribozymes), energy transfer (e.g., ATP) and information storage (RNA and DNA) biomolecules. Planetary models suggest that molecular nitrogen was abundant in the early Martian atmosphere, but was rapidly lost to space by photochemistry, sputtering impact erosion, and oxidized and deposited to the surface as nitrate. Nitrates are a fundamental source for nitrogen to terrestrial microorganisms. Therefore, the detection of nitrates in soils and rocks is important to assess the habitability of a Martian environment. SAM is capable of detecting nitrates by their thermal decomposition into nitric oxide, NO. Here we analyze the release of NO from soils and rocks examined by the SAM instrument at Gale crater, and discuss its origin.

  9. Shear-Induced Detachment of Polystyrene Beads from SAM-Coated Surfaces.

    PubMed

    Cho, Kwun Lun; Rosenhahn, Axel; Thelen, Richard; Grunze, Michael; Lobban, Matthew; Karahka, Markus Leopold; Kreuzer, H Jürgen

    2015-10-13

    In this work we experimentally and theoretically analyze the detachment of microscopic polystyrene beads from different self-assembled monolayer (SAM) surfaces in a shear flow in order to develop a mechanistic model for the removal of cells from surfaces. The detachment of the beads from the surface is treated as a thermally activated process applying an Arrhenius Ansatz to determine the activation barrier and attempt frequency of the rate determing step in bead removal. The statistical analysis of the experimental shear detachment data obtained in phosphate-buffered saline buffer results in an activation energy around 20 kJ/mol, which is orders of magnitude lower than the adhesion energy measured by atomic force microscopy (AFM). The same order of magnitude for the adhesion energy measured by AFM is derived from ab initio calculations of the van der Waals interaction energy between the polystyrene beads and the SAM-covered gold surface. We conclude that the rate determing step for detachment of the beads is the initiation of rolling on the surface (overcoming static friction) and not physical detachment, i.e., lifting the particle off the surface. PMID:26401759

  10. Non-canonical active site architecture of the radical SAM thiamin pyrimidine synthase

    SciTech Connect

    Fenwick, Michael K.; Mehta, Angad P.; Zhang, Yang; Abdelwahed, Sameh H.; Begley, Tadhg P.; Ealick, Steven E.

    2015-03-27

    Radical S-adenosylmethionine (SAM) enzymes use a [4Fe-4S] cluster to generate a 5'-deoxyadenosyl radical. Canonical radical SAM enzymes are characterized by a β-barrel-like fold and SAM anchors to the differentiated iron of the cluster, which is located near the amino terminus and within the β-barrel, through its amino and carboxylate groups. Here we show that ThiC, the thiamin pyrimidine synthase in plants and bacteria, contains a tethered cluster-binding domain at its carboxy terminus that moves in and out of the active site during catalysis. In contrast to canonical radical SAM enzymes, we predict that SAM anchors to an additional active site metal through its amino and carboxylate groups. Superimposition of the catalytic domains of ThiC and glutamate mutase shows that these two enzymes share similar active site architectures, thus providing strong evidence for an evolutionary link between the radical SAM and adenosylcobalamin-dependent enzyme superfamilies.

  11. GABAergic inhibition shapes SAM responses in rat auditory thalamus.

    PubMed

    Cai, R; Caspary, D M

    2015-07-23

    Auditory thalamus (medial geniculate body [MGB]) receives ascending inhibitory GABAergic inputs from inferior colliculus (IC) and descending GABAergic projections from the thalamic reticular nucleus (TRN) with both inputs postulated to play a role in shaping temporal responses. Previous studies suggested that enhanced processing of temporally rich stimuli occurs at the level of MGB, with our recent study demonstrating enhanced GABA sensitivity in MGB compared to IC. The present study used sinusoidal amplitude-modulated (SAM) stimuli to generate modulation transfer functions (MTFs), to examine the role of GABAergic inhibition in shaping the response properties of MGB single units in anesthetized rats. Rate MTFs (rMTFs) were parsed into "bandpass (BP)", "mixed (Mixed)", "highpass (HP)" or "atypical" response types, with most units showing the Mixed response type. GABAA receptor blockade with iontophoretic application of the GABAA receptor (GABAAR) antagonist gabazine (GBZ) selectively altered the response properties of most MGB neurons examined. Mixed and HP units showed significant GABAAR-mediated SAM-evoked rate response changes at higher modulation frequencies (fms), which were also altered by N-methyl-d-aspartic acid (NMDA) receptor blockade (2R)-amino-5-phosphonopentanoate (AP5). BP units, and the lower arm of Mixed units responded to GABAAR blockade with increased responses to SAM stimuli at or near the rate best modulation frequency (rBMF). The ability of GABA circuits to shape responses at higher modulation frequencies is an emergent property of MGB units, not observed at lower levels of the auditory pathway and may reflect activation of MGB NMDA receptors (Rabang and Bartlett, 2011; Rabang et al., 2012). Together, GABAARs exert selective rate control over selected fms, generally without changing the units' response type. These results showed that coding of modulated stimuli at the level of auditory thalamus is at least, in part, strongly controlled by GABA

  12. Paramagnetic Intermediates Generated by Radical S-Adenosylmethionine (SAM) Enzymes

    PubMed Central

    2015-01-01

    Conspectus A [4Fe–4S]+ cluster reduces a bound S-adenosylmethionine (SAM) molecule, cleaving it into methionine and a 5′-deoxyadenosyl radical (5′-dA•). This step initiates the varied chemistry catalyzed by each of the so-called radical SAM enzymes. The strongly oxidizing 5′-dA• is quenched by abstracting a H-atom from a target species. In some cases, this species is an exogenous molecule of substrate, for example, l-tyrosine in the [FeFe] hydrogenase maturase, HydG. In other cases, the target is a proteinaceous residue as in all the glycyl radical forming enzymes. The generation of this initial radical species and the subsequent chemistry involving downstream radical intermediates is meticulously controlled by the enzyme so as to prevent unwanted reactions. But the manner in which this control is exerted is unknown. Electron paramagnetic resonance (EPR) spectroscopy has proven to be a valuable tool used to gain insight into these mechanisms. In this Account, we summarize efforts to trap such radical intermediates in radical SAM enzymes and highlight four examples in which EPR spectroscopic results have shed significant light on the corresponding mechanism. For lysine 2,3-aminomutase, nearly each possible intermediate, from an analogue of the initial 5′-dA• to the product radical l-β-lysine, has been explored. A paramagnetic intermediate observed in biotin synthase is shown to involve an auxiliary [FeS] cluster whose bridging sulfide is a co-substrate for the final step in the biosynthesis of vitamin B7. In HydG, the l-tyrosine substrate is converted in unprecedented fashion to a 4-oxidobenzyl radical on the way to generating CO and CN– ligands for the [FeFe] cluster of hydrogenase. And finally, EPR has confirmed a mechanistic proposal for the antibiotic resistance protein Cfr, which methylates the unactivated sp2-hybridized C8-carbon of an adenosine base of 23S ribosomal RNA. These four systems provide just a brief survey of the ever-growing set

  13. Uranium from phosphoric acid: IMC`s Uncle Sam Plant

    SciTech Connect

    1994-02-01

    This article discusses uranium recovery from phosphoric acid, proven to be a viable technology by several U.S. producers since 1978. This technology has accounted for 12.8% of U.S. uranium production during this time: a total of almost 40 Mlb equivalent U3O8. Of the several producers, only the Uncle Sam plant of IMC-Agrico has operated continuously during the period, and that plant is the longest-lived uranium production facility operating in the United States. The basis for the process is reviewed, including geological aspects, mining and recovery of phosphorite, phosphoric acid production, and uranium recovery. Licensing of such facilities is also discussed.

  14. Comparative studies of aerosol extinction measurements made by the SAM II and SAGE II satellite experiments

    NASA Technical Reports Server (NTRS)

    Yue, Glenn K.; Mccormick, M. P.; Chu, W. P.; Wang, P.; Osborn, M. T.

    1989-01-01

    Results from the Stratospheric Aerosol Measurement (SAM) II and Stratospheric Aerosol and Gas Experiment (SAGE) II are compared for measurement locations which are coincident in time and space. At 1.0 micron, the SAM II and SAGE II aerosol extinction profiles are similar within their measurement errors. In addition, sunrise and sunset aerosol extinction data at four different wavelengths are compared for occasions when the SAGE II and SAM II measurements are nearly coincident in space and about 12 hours apart.

  15. The nuclear protein Sam68 is cleaved by the FMDV 3C protease redistributing Sam68 to the cytoplasm during FMDV infection of host cells

    SciTech Connect

    Lawrence, Paul; Schafer, Elizabeth A.; Rieder, Elizabeth

    2012-03-30

    Picornavirus infection can lead to disruption of nuclear pore traffic, shut-off of cell translation machinery, and cleavage of proteins involved in cellular signal transduction and the innate response to infection. Here, we demonstrated that the FMDV 3C{sup pro} induced the cleavage of nuclear RNA-binding protein Sam68 C-terminus containing the nuclear localization sequence (NLS). Consequently, it stimulated the redistribution of Sam68 to the cytoplasm. The siRNA knockdown of Sam68 resulted in a 1000-fold reduction in viral titers, which prompted us to study the effect of Sam68 on FMDV post-entry events. Interestingly, Sam68 interacts with the internal ribosomal entry site within the 5 Prime non-translated region of the FMDV genome, and Sam68 knockdown decreased FMDV IRES-driven activity in vitro suggesting that it could modulate translation of the viral genome. The results uncover a novel role for Sam68 in the context of picornaviruses and the proteolysis of a new cellular target of the FMDV 3C{sup pro}.

  16. 78 FR 16259 - Subcommittee Meeting of the Board of Advisors to the President, Naval Postgraduate School

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-14

    ... Advisors (BOA) to the Presidents of the Naval Postgraduate School and the Naval War College (NPS). This... meeting location. For access, information, or to send written comments regarding the NPS BOA contact...

  17. The CBT Advisor: An Expert System Program for Making Decisions about CBT.

    ERIC Educational Resources Information Center

    Kearsley, Greg

    1985-01-01

    Discusses structure, credibility, and use of the Computer Based Training (CBT) Advisor, an expert system designed to help managers make judgements about course selection, system selection, cost/benefits, development effort, and probable success of CBT projects. (MBR)

  18. The App Squad: SLJ's Advisors Weigh in on Kids' Book Apps

    ERIC Educational Resources Information Center

    Ishizuka, Kathy

    2011-01-01

    In this article, "School Library Journal's" ("SLJ") advisors talk about book apps for kids. They discuss what they like, what one should look for in discerning the best for kids and teens, and where this all might be headed.

  19. The first year: Development of a LANDSAT capability at Sam Houston State University

    NASA Technical Reports Server (NTRS)

    Bounds, J. H.

    1981-01-01

    Problems encountered in initiating a LANDSAT data processing capability at Sam Houston State University are discussed. Computer requirements, financing, and academic and administrative support are addressed.

  20. Sam68 is cleaved by caspases under apoptotic cell death induced by ionizing radiation.

    PubMed

    Cho, Seong-Jun; Choi, Moo Hyun; Nam, Seon Young; Kim, Ji Young; Kim, Cha Soon; Pyo, Suhkneung; Yang, Kwang Hee

    2015-03-01

    The RNA-binding protein Sam68, a mitotic substrate of tyrosine kinases, has been reported to participate in the cell cycle, apoptosis, and signaling. In particular, overexpression of Sam68 protein is known to suppress cell growth and cell cycle progression in NIH3T3 cells. Although Sam68 is involved in many cellular activities, the function of Sam68, especially in response to apoptotic stimulation, is not well understood. In this study, we found that Sam68 protein is cleaved in immune cells undergoing apoptosis induced by γ-radiation. Moreover, we found that Sam68 cleavage was induced by apoptotic stimuli containing γ-radiation in a caspase-dependent manner. In particular, we showed that activated casepase-3, 7, 8 and 9 can directly cleave Sam68 protein through in vitro protease cleavage assay. Finally, we found that the knockdown of Sam68 attenuated γ-radiation-induced cell death and growth suppression. Conclusively, the cleavage of Sam68 is a new indicator for the cell damaging effects of ionizing radiation. PMID:25666188

  1. What Are Faculty Advisors To Do When Their Own Career Path Does Not Satisfy Their Students?

    NASA Astrophysics Data System (ADS)

    McManus, D. A.

    2001-12-01

    As graduate students seek advice on broad career options, many faculty advisors do not know what to do. It is easy for them to do nothing. They may do nothing because they assume that their own students are interested only in an academic research career like theirs. The mistake here can be that the advisors' verbal and non-verbal communication deters students from mentioning their interests in the first place or pursuing those interests, if mentioned. Or advisors may do nothing by assuming that it is not their responsibility to advise students about career options other than being an academic researcher. The advisors' lack of knowledge about other careers may lead them to avoid the issue. The mistake here is obvious. So what are advisors to do? They can encourage students to think of their graduate study as part of their career preparation, not just a task to obtain a research degree. Creating a risk-free environment for career discussion will enable faculty advisors to learn each student's career priorities and validate exploration of broad career options. Advisors should not feel inadequate by being unable to advise about everything. No one expects them to. They can encourage their students to meet together, on their own if necessary, to discuss common career concerns, even to invite speakers, including alums, to talk about different careers and the preparation required. They can encourage their students to seek additional mentors, people more knowledgeable about careers of interest to the students. They can encourage students to take courses for career preparation, particularly courses outside of science, even though these courses "take them away from their research." And advisors should not hold students at fault if they change their minds about career paths. More information often changes minds. These are a few of the many things that advisors can do. It is essential that faculty advisors not resent students' decisions to follow a career path different from

  2. Confessions of a President's Science Advisor (George B. Pegram Lecture Series)

    SciTech Connect

    Lane, Neal

    2006-09-11

    Former science advisor to president Bill Clinton Neal Lane briefly reviews the history of the job of Science Advisor to the President and give some examples of issues he had to deal with when he was in that position, including climate change, stem cell research, the human genome, nanotechnology and research funding. He will also give his opinions about the present and future state of science in the U.S.

  3. Tertiary contacts control switching of the SAM-I riboswitch.

    PubMed

    Hennelly, Scott P; Sanbonmatsu, Karissa Y

    2011-03-01

    Riboswitches are non-coding RNAs that control gene expression by sensing small molecules through changes in secondary structure. While secondary structure and ligand interactions are thought to control switching, the exact mechanism of control is unknown. Using a novel two-piece assay that competes the anti-terminator against the aptamer, we directly monitor the process of switching. We find that the stabilization of key tertiary contacts controls both aptamer domain collapse and the switching of the SAM-I riboswitch from the aptamer to the expression platform conformation. Our experiments demonstrate that SAM binding induces structural alterations that indirectly stabilize the aptamer domain, preventing switching toward the expression platform conformer. These results, combined with a variety of structural probing experiments performed in this study, show that the collapse and stabilization of the aptamer domain are cooperative, relying on the sum of key tertiary contacts and the bimodal stability of the kink-turn motif for function. Here, ligand binding serves to shift the equilibrium of aptamer domain structures from a more open toward a more stable collapsed form by stabilizing tertiary interactions. Our data show that the thermodynamic landscape for riboswitch operation is finely balanced to allow large conformational rearrangements to be controlled by small molecule interactions. PMID:21097777

  4. Tertiary contacts control switching of the SAM-I riboswitch

    PubMed Central

    Hennelly, Scott P.; Sanbonmatsu, Karissa Y.

    2011-01-01

    Riboswitches are non-coding RNAs that control gene expression by sensing small molecules through changes in secondary structure. While secondary structure and ligand interactions are thought to control switching, the exact mechanism of control is unknown. Using a novel two-piece assay that competes the anti-terminator against the aptamer, we directly monitor the process of switching. We find that the stabilization of key tertiary contacts controls both aptamer domain collapse and the switching of the SAM-I riboswitch from the aptamer to the expression platform conformation. Our experiments demonstrate that SAM binding induces structural alterations that indirectly stabilize the aptamer domain, preventing switching toward the expression platform conformer. These results, combined with a variety of structural probing experiments performed in this study, show that the collapse and stabilization of the aptamer domain are cooperative, relying on the sum of key tertiary contacts and the bimodal stability of the kink-turn motif for function. Here, ligand binding serves to shift the equilibrium of aptamer domain structures from a more open toward a more stable collapsed form by stabilizing tertiary interactions. Our data show that the thermodynamic landscape for riboswitch operation is finely balanced to allow large conformational rearrangements to be controlled by small molecule interactions. PMID:21097777

  5. Redox regulation in shoot growth, SAM maintenance and flowering.

    PubMed

    Schippers, Jos Hm; Foyer, Christine H; van Dongen, Joost T

    2016-02-01

    Reactive oxygen species (ROS) and associated reduction/oxidation (redox) controls involving glutathione, glutaredoxins and thioredoxins play key roles in the regulation of plant growth and development. While many questions remain concerning redox functions in the shoot apical meristem (SAM), accumulating evidence suggests that redox master switches integrate major hormone signals and transcriptional networks in the SAM, and so regulate organ growth, polarity and floral development. Auxin-induced activation of plasma-membrane located NADPH-oxidases and mitochondrial respiratory bioenergetics are likely regulators of the ROS bursts that drive the cell cycle in proliferating regions, with other hormones such as jasmonic acid playing propagating or antagonistic roles in gene regulation. Moreover, the activation of oxygen production by photosynthesis and oxygen-dependent N-end rule controls are linked to the transition from cell proliferation to cell expansion and differentiation. While much remains to be understood, the nexus of available redox controls provides a key underpinning mechanism linking hormonal controls, energy metabolism and bioenergetics to plant growth and development. PMID:26799134

  6. Facing page test for the astronaut science advisor presentation

    NASA Technical Reports Server (NTRS)

    Compton, Michael M.

    1991-01-01

    The goal of the Astronaut Science Advisor (ASA) project is to improve the scientific return of experiments performed in space by providing astronaut experimenters with an 'intelligent assistant' that encapsulates much of the domain- and experiment-related knowledge commanded by the Principal Investigator (PI) on the ground. By using expert systems technology and the availability of flight-qualified personal computers, it is possible to encode the requisite knowledge and make it available to astronauts as they perform experiments in space. The system performs four major functions: diagnosis and troubleshooting of experiment apparatus, data collection, protocol management, and detection of interesting data. The experiment used for development of the system measures human adaptation to weightlessness in the context of the neurovestibular system. This so-called 'Rotating Dome' experiment was flown on the recent Spacelab Life Sciences One (SLS-1) Mission. This mission was used as an opportunity to test some of the system's functionality. Experiment data was downlinked from the orbiter, and the system then captured the data and analyzed it in real time. The system kept track of the time being used by the experiment, recognized occurrences of interesting data, summarized data statistically and generated potential new protocols that could be used to optimize the course of the experiment.

  7. In situ analysis of Mars soil sample with the sam gcms instrumentation onboard Curiosity : interpretation and comparison of measurements done at Rocknest and Yelloknife bay sites

    NASA Astrophysics Data System (ADS)

    Szopa, Cyril; Coll, Patrice; Cabane, Michel; Coscia, David; Buch, Arnaud; Francois, Pascaline; Millan, Maeva; Teinturier, Sammy; Navarro-Gonzales, Rafael; Glavin, Daniel; Freissinet, Caro; Steele, Andrew; Eigenbrode, Jen; Mahaffy, Paul

    2014-05-01

    The characterisation of the chemical and mineralogical composition of regolith samples collected with the Curiosity rover is a primary objective of the SAM experiment. These data should provide essential clues on the past habitability of Gale crater. Amongst the SAM suite of instruments [1], SAM-GC (Gas Chromatograph) is devoted to identify and quantify volatiles evolved from the thermal (heating up to about 900°C)/chemical (derivatization procedure) treatment of any soil sample collected by the Curiosity rover. With the aim to search for potential organic molecules outgassed from the samples, a SAM-GC analytical channel composed of thermal-desorption injector and a MXT-CLP chromatographic column was chosen to achieve all the measurements done up today, as it was designed for the separation of a wide range of volatile organic molecules. Three solid samples have been analyzed with GCMS, one sand sample collected at the Rocknest site, and two rock samples (John Klein and Cumberland respectively) collected at the Yellowknife Bay site using the Curiosity driller. All the measurements were successful and they produced complex chromatograms with both detectors used for SAM GC, i.e. a thermal conductivity detector and the SAM quandrupole mass spectrometer. Their interpretation already revealed the presence of an oxychlorine phase present in the sample which is at the origin of chlorohydrocarbons clearly identified [2] but this represents only a fraction of the GCMS signal recorded [3,4]. This work presents a systematic comparison of the GCMS measurements done for the different samples collected, supported by reference data obtained in laboratory with different spare models of the gas chromatograph, with the aim to bring new elements of interpretation of the SAM measurements. References: [1] Mahaffy, P. et al. (2012) Space Sci Rev, 170, 401-478. [2] Glavin, D. et al. (2013), JGR. [3] Leshin L. et al. (2013), Science, [4] Ming D. et al. (2013), Science, 32, 64

  8. Sam68 Regulates S6K1 Alternative Splicing during Adipogenesis

    PubMed Central

    Song, Jingwen

    2015-01-01

    The requirement for alternative splicing during adipogenesis is poorly understood. The Sam68 RNA binding protein is a known regulator of alternative splicing, and mice deficient for Sam68 exhibit adipogenesis defects due to defective mTOR signaling. Sam68 null preadipocytes were monitored for alternative splicing imbalances in components of the mTOR signaling pathway. Herein, we report that Sam68 regulates isoform expression of the ribosomal S6 kinase gene (Rps6kb1). Sam68-deficient adipocytes express Rps6kb1-002 and its encoded p31S6K1 protein, in contrast to wild-type adipocytes that do not express this isoform. Sam68 binds an RNA sequence encoded by Rps6kb1 intron 6 and prevents serine/arginine-rich splicing factor 1 (SRSF1)-mediated alternative splicing of Rps6kb1-002, as assessed by cross-linking and immunoprecipitation (CLIP) and minigene assays. Depletion of p31S6K1 with small interfering RNAs (siRNAs) partially restored adipogenesis of Sam68-deficient preadipocytes. The ectopic expression of p31S6K1 in wild-type 3T3-L1 cells resulted in adipogenesis differentiation defects, showing that p31S6K1 is an inhibitor of adipogenesis. Our findings indicate that Sam68 is required to prevent the expression of p31S6K1 in adipocytes for adipogenesis to occur. PMID:25776557

  9. Regioselective patterning of multiple SAMs and applications in surface-guided smart microfluidics.

    PubMed

    Chen, Chuanzhao; Xu, Pengcheng; Li, Xinxin

    2014-12-24

    A top-down nanofabrication technology is developed to integrate multiple SAMs (self-assembled monolayers) into regioselective patterns. With ultraviolet light exposure through regioselectively hollowed hard mask, an existing SAM at designated microregions can be removed and a dissimilar kind of SAM can be regrown there. By repeating the photolithography-like process cycle, diverse kinds of SAM building blocks can be laid out as a desired pattern in one microfluidic channel. In order to ensure high quality of the surface modifications, the SAMs are vapor-phase deposited before the channel is closed by a bonding process. For the first time the technique makes it possible to integrate three or more kinds of SAMs in one microchannel. The technique is very useful for multiplex surface functionalization of microfluidic chips where different segments of a microfluidic channel need to be individually modified with different SAMs or into arrayed pattern for surface-guided fluidic properties like hydrophobicity/philicity and/or oleophobicity/philicity, etc. The technique has been well validated by experimental demonstration of various surface-directed flow-guiding functions. By modifying a microchannel surface into an arrayed pattern of multi-SAM "two-tone" stripe array, surface-guiding-induced 3D swirling flow is generated in a microfluidic channel that experimentally exhibits quick oil/water mixing and high-efficiency oil-to-water chemical extraction. PMID:25438296

  10. Overview of SAM results obtained at Gale Crater during the 180 first sols

    NASA Astrophysics Data System (ADS)

    Coll, P.; Mahaffy, P. R.; Cabane, M.; Webster, C. R.; Archer, D.; Atreya, S. K.; Benna, M.; Brinckerhoff, W. B.; Brunner, A.; Buch, A.; Conrad, P.; Coscia, D.; Dobson, N.; Dworkin, J.; Eigenbrode, J.; Farley, K.; Flesch, G.; Franz, H.; Freissinet, C.; Galvin, D.; Gorevan, S.; Harpold, D.; Hengemihle, J.; Jaeger, F.; Johnson, C.; Johnson, M.; Jones, J.; Lefavor, M.; Leshin, L.; Lyness, E.; Malespin, C.; Manning, H.; Martin, D.; McAdam, A.; McKay, C.; Miller, K.; Ming, D. W.; Morris, R. V.; Navarro-González, R.; Niles, P.; Nolan, T.; Owen, T.; Pavolv, A.; Prats, B.; Pepin, R.; Raaen, E.; Raulin, F.; Steele, A.; Stern, J.; Squyres, S.; Sutter, B.; Summons, R. E.; Szopa, C.; Tan, F.; Teinturier, S.; Trainer, M.; Wong, M.; Wray, J.

    2013-09-01

    During the first 180 sols of Curiosity's landed mission on Mars (8/6/2012 to 2/7/2013) SAM sampled the atmosphere more than a dozen times, the dusty sandpile named Rocknest and a basin site named John Klein on the floor of Gale crater. The atmospheric experiments utilized SAM's quadrupole mass spectrometer (QMS) and its tunable laser spectrometer (TLS) while the solid sample experiments also utilized the gas chromatograph (GC). Although a number of core experiments were pre-programmed and stored in SAM EEProm, the high level SAM scripting language enabled the team to often optimize experiments based on prior runs. SAM and its Experiment Sequences exercised during the First 120 Sols: The SAM instruments, its gas processing system (GPS) and its sample manipulation system (SMS) have been already described [1]. During the first few weeks of the landed mission SAM carried out a variety of instrument health checks and then began a series of atmospheric experiments to measure atmospheric composition and isotope ratios. From sol 56 to 102 Curiosity lingered at Rocknest to clean out the surfaces of the sample processing system by scooping several times into this fine grained material, vibrating to abrade possible contamination from surfaces, and then discarding before delivery of sample to SAM from the 5th scoop.

  11. 77 FR 19691 - Notice of Inventory Completion: Sam Noble Oklahoma Museum of Natural History, Norman, OK

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-02

    ... National Park Service Notice of Inventory Completion: Sam Noble Oklahoma Museum of Natural History, Norman... Natural History has completed an inventory of human remains and associated funerary objects, in... associated funerary objects may contact the Sam Noble Oklahoma Museum of Natural History. Repatriation of...

  12. Unanticipated coordination of tris buffer to the Radical SAM cluster of the RimO methylthiotransferase.

    PubMed

    Molle, Thibaut; Clémancey, Martin; Latour, Jean-Marc; Kathirvelu, Velavan; Sicoli, Giuseppe; Forouhar, Farhad; Mulliez, Etienne; Gambarelli, Serge; Atta, Mohamed

    2016-07-01

    Radical SAM enzymes generally contain a [4Fe-4S](2+/1+) (RS cluster) cluster bound to the protein via the three cysteines of a canonical motif CxxxCxxC. The non-cysteinyl iron is used to coordinate SAM via its amino-carboxylate moiety. The coordination-induced proximity between the cluster acting as an electron donor and the adenosyl-sulfonium bond of SAM allows for the homolytic cleavage of the latter leading to the formation of the reactive 5'-deoxyadenosyl radical used for substrate activation. Most of the structures of Radical SAM enzymes have been obtained in the presence of SAM, and therefore, little is known about the situation when SAM is not present. In this report, we show that RimO, a methylthiotransferase belonging to the radical SAM superfamily, binds a Tris molecule in the absence of SAM leading to specific spectroscopic signatures both in Mössbauer and pulsed EPR spectroscopies. These data provide a cautionary note for researchers who work with coordinative unsaturated iron sulfur clusters. PMID:27259294

  13. Winning Attitude & Dedication to Physical Therapy Keep Sam Schmidt on Track

    ERIC Educational Resources Information Center

    Bosley, Nikki Prevenslik

    2006-01-01

    This article relates how Sam Schmidt returned to living a productive life after an accident left him with spinal cord injury. Schmidt was a former Indy Racing League driver who founded Sam Schmidt Motorsports after his accident in 2000. Schmidt's car hit the wall as he exited turn two during a practice session at Walt Disney World Speedway in…

  14. Radical SAM catalysis via an organometallic intermediate with an Fe-[5'-C]-deoxyadenosyl bond.

    PubMed

    Horitani, Masaki; Shisler, Krista; Broderick, William E; Hutcheson, Rachel U; Duschene, Kaitlin S; Marts, Amy R; Hoffman, Brian M; Broderick, Joan B

    2016-05-13

    Radical S-adenosylmethionine (SAM) enzymes use a [4Fe-4S] cluster to cleave SAM to initiate diverse radical reactions. These reactions are thought to involve the 5'-deoxyadenosyl radical intermediate, which has not yet been detected. We used rapid freeze-quenching to trap a catalytically competent intermediate in the reaction catalyzed by the radical SAM enzyme pyruvate formate-lyase activating enzyme. Characterization of the intermediate by electron paramagnetic resonance and (13)C, (57)Fe electron nuclear double-resonance spectroscopies reveals that it contains an organometallic center in which the 5' carbon of a SAM-derived deoxyadenosyl moiety forms a bond with the unique iron site of the [4Fe-4S] cluster. Discovery of this intermediate extends the list of enzymatic bioorganometallic centers to the radical SAM enzymes, the largest enzyme superfamily known, and reveals intriguing parallels to B12 radical enzymes. PMID:27174986

  15. S.A.M., the Italian Martian Simulation Chamber

    NASA Astrophysics Data System (ADS)

    Galletta, G.; Ferri, F.; Fanti, G.; D'Alessandro, M.; Bertoloni, G.; Pavarin, D.; Bettanini, C.; Cozza, P.; Pretto, P.; Bianchini, G.; Debei, S.

    2006-12-01

    The Martian Environment Simulator (SAM “Simulatore di Ambiente Marziano”) is a interdisciplinary project of Astrobiology done at University of Padua. The research is aimed to the study of the survival of the microorganisms exposed to the “extreme” planetary environment. The facility has been designed in order to simulate Mars’ environmental conditions in terms of atmospheric pressure, temperature cycles and UV radiation dose. The bacterial cells, contained into dedicated capsules, will be exposed to thermal cycles simulating diurnal and seasonal Martian cycles. The metabolism of the different biological samples will be analysed at different phases of the experiment, to study their survival and eventual activity of protein synthesis (mortality, mutations and capability of DNA reparing). We describe the experimental facility and provide the perspectives of the biological experiments we will perform in order to provide hints on the possibility of life on Mars either autochthonous or imported from Earth.

  16. Presentation on a Space Acceleration Measurement System (SAMS)

    NASA Technical Reports Server (NTRS)

    Chase, Theodore L.

    1990-01-01

    The primary objective of the Space Acceleration Measurement Systems (SAMS) project is to provide an acceleration measurement system capable of serving a wide variety of space experiments. The design of the system being developed under this project takes into consideration requirements for experiments located in the middeck, in the orbiter bay, and in Spacelab. In addition to measuring, conditioning, and recording accelerations, the system will be capable of performing complex calculations and interactive control. The main components consist of a remote triaxial optical storage device. In operation, the triaxial sensor head produces output signals in response to acceleration inputs. These signals are preamplified, filtered and converted into digital data which is then transferred to optical memory. The system design is modular, facilitating both software and hardware upgrading as technology advances. Two complete acceleration measurement flight systems will be build and tested under this project.

  17. Photon multiplication in wide-gap BAM and SAM aluminates

    NASA Astrophysics Data System (ADS)

    Lushchik, Aleksandr; Lushchik, Cheslav; Feldbach, Eduard; Kudryavtseva, Irina; Liblik, Peeter; Maaroos, Aarne; Nagirnyi, Vitali; Savikhin, Fjodor; Vasil'chenko, Eugeni

    2005-08-01

    Processes of various intrinsic and impurity luminescence excitation by 4-32 eV photons or 18 and 300 keV electrons have been studied in pure and doped BaMgAl10O17 (BAM) and SrMgAl10O17 (SAM) phosphors at 6-300 K. In BAM:Eu (l0%), the quantum yield of Eu2+ center emission is QY = 1 in the region of exciting photon energies of hνec = 7-12 eV, the value of QY reaches 2 at 14-21 eV and sharply increases at hνec = 22-32 eV, where secondary electron-hole pairs are created by hot conduction electrons. The processes connected with the rise of QY for various types of emission in the region of 14-21 eV have been thoroughly studied for BAM and SAM phosphors. It has been suggested that such exciting photons cause the ionization of oxygen ions and form hot valence holes, the energy of which is partially used for the excitation of Eu2+ ions (4f7→4f65d1 transitions) due to nonradiative Auger transitions. The intensity of the Eu2+ emission increases after a single nanosecond electron pulse with a rise time of 50-150 ns. This rise is connected with the energy transfer from spinel blocks to Eu2+ ions located at cation planes of the β-alumina-type materials.

  18. Social activity method (SAM): A fractal language for mathematics

    NASA Astrophysics Data System (ADS)

    Dowling, Paul

    2013-09-01

    In this paper I shall present and develop my organisational language, social activity method (SAM), and illustrate some of its applications. I shall introduce a new scheme for modes of recontextualisation that enables the analysis of the ways in which one activity - which might be school mathematics or social research or any empirically observed regularity of practice - recontextualises the practice of another and I shall also present, deploy, and develop an existing scheme - domains of action - in an analysis of school mathematics examination papers and in the structuring of what I refer to as the esoteric domain. This domain is here conceived as a hybrid domain of, first, linguistic and extralinguistic resources that are unambiguously mathematical in terms of both expression and content and, second, pedagogic theory - often tacit - that enables the mathematical gaze onto other practices and so recontextualises them. A second and more general theme that runs through the paper is the claim that there is nothing that is beyond semiosis, that there is nothing to which we have direct access, unmediated by interpretation. This state of affairs has implications for mathematics education. Specifically, insofar as an individual's mathematical semiotic system is under continuous development - the curriculum never being graspable all at once - understanding - as a stable semiotic moment - of any aspect or object of mathematics is always localised to the individual and is at best transient, and the sequencing of such moments may well also be more individualised than consistent in some correspondence with the sequencing of the curriculum. This being the case, a concentration on understanding as a goal may well serve to inhibit the pragmatic acquisition and deployment of mathematical technologies, which should be the principal aim of mathematics teaching and learning. The paper is primarily concerned with mathematics education. SAM, however, is a language that is available for

  19. Therapy planning as constraint satisfaction: a computer-based antiretroviral therapy advisor for the management of HIV.

    PubMed Central

    Smith, D. S.; Park, J. Y.; Musen, M. A.

    1998-01-01

    We applied the Protégé methodology for building knowledge-based systems to the domain of antiretroviral therapy. We modeled the task of prescribing drug therapy for HIV, abstracting the essential characteristics of the problem solving. We mapped our model of the antiretroviral-therapy domain to the class of constraint-satisfaction problems, and reused the propose-and-revise problem-solving method, from the Protégé library of methods, to build an antiretroviral therapy advisor, ART Critic. Careful modeling and using Protégé allowed us to build a useful and extensible knowledge-based application rapidly. PMID:9929295

  20. Battery thermal models for hybrid vehicle simulations

    NASA Astrophysics Data System (ADS)

    Pesaran, Ahmad A.

    This paper summarizes battery thermal modeling capabilities for: (1) an advanced vehicle simulator (ADVISOR); and (2) battery module and pack thermal design. The National Renewable Energy Laboratory's (NREL's) ADVISOR is developed in the Matlab/Simulink environment. There are several battery models in ADVISOR for various chemistry types. Each one of these models requires a thermal model to predict the temperature change that could affect battery performance parameters, such as resistance, capacity and state of charges. A lumped capacitance battery thermal model in the Matlab/Simulink environment was developed that included the ADVISOR battery performance models. For thermal evaluation and design of battery modules and packs, NREL has been using various computer aided engineering tools including commercial finite element analysis software. This paper will discuss the thermal ADVISOR battery model and its results, along with the results of finite element modeling that were presented at the workshop on "Development of Advanced Battery Engineering Models" in August 2001.

  1. Roles of religious and spiritual advisors among adults in Singapore with mental illnesses.

    PubMed

    Picco, Louisa; Subramaniam, Mythily; Abdin, Edimansyah; Vaingankar, Janhavi Ajit; Zhang, Yunjue; Chong, Siow Ann

    2013-11-01

    OBJECTIVE Information is limited concerning the role of religious and spiritual advisors in providing help to people with mental illnesses in Singapore. This study examined that role, as well as the satisfaction with and the perceived effectiveness of the services provided, among people with mental health problems. METHODS Data were collected as part of a nationally representative household survey of residents 18 years and older in Singapore. The Composite International Diagnostic Interview, version 3.0, was used to diagnose mental illness as well as to collect information about the mental health services respondents had sought. RESULTS A total of 6,616 respondents completed the survey; in the overall sample, 1.5% reported seeking help from religious or spiritual advisors. This rate increased to 6.6% among those with at least one mental illness, with the prevalence being higher among respondents with lifetime dysthymia, generalized anxiety disorder, or bipolar disorder. Sociodemographic correlates associated with a lower likelihood of consultation with a religious or spiritual advisor included reporting "other" race-ethnicity as well as faith in Buddhism, Hinduism, or Islam. Most respondents who sought help from a religious or spiritual advisor in the last 12 months were satisfied with the help they received, and about half reported it to be very useful. CONCLUSIONS Religious and spiritual advisors are an important source of help for people with mental illness, and a majority of respondents with a mental illness were satisfied with the support they received from these sources. PMID:23903293

  2. Nonlinear Optical Studies of Self-Assembled Monolayers (SAM) Silica-SAM-Water Interface Probed With Second Harmonic Generation

    NASA Astrophysics Data System (ADS)

    James, Kenneth

    2010-03-01

    Second harmonic generation (SHG) is a successful and widely used technique for the study of surfaces and surface phenomena. We present a novel technique using second harmonic generation from oriented water molecules in the Gouy-Chapman diffuse layer at the alkylsiloxane and biomolecular self assembled monolayer (SAM) interface with water to measure distance between the solid surface and the average location of the oriented water in the diffuse layer. Distances of one nanometer can be distinguished. This in situ probe is applicable for organic adsorbates which in general will push the diffuse layer away from the solid surface. The organic layer thickness can be used to obtain the adsorption fraction. From this and an understanding of the likely chemistry, the orientation of the molecules can be inferred. We have demonstrated this technique on three molecular systems: hydrophobic self assembled monolayers of methoxysilane molecules of varying hydrocarbon chain length, self assembled monolayers of streptavidin glycoproteins and the combined streptavidin-biotinylated antibody monolayer.

  3. Streptomyces serine protease (SAM-P20): recombinant production, characterization, and interaction with endogenous protease inhibitor.

    PubMed Central

    Taguchi, S; Suzuki, M; Kojima, S; Miura, K; Momose, H

    1995-01-01

    Previously, we isolated a candidate for an endogenous target enzyme(s) of the Streptomyces subtilisin inhibitor (SSI), termed SAM-P20, from a non-SSI-producing mutant strain (S. Taguchi, A. Odaka, Y. Watanabe, and H. Momose, Appl. Environ. Microbiol. 61:180-186, 1995). In this study, in order to investigate the detailed enzymatic properties of this protease, an overproduction system of recombinant SAM-P20 was established in Streptomyces coelicolor with the SSI gene promoter. The recombinant SAM-P20 was purified by salting out and by two successive ion-exchange chromatographies to give a homogeneous band by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Partial peptide mapping and amino acid composition analysis revealed that the recombinant SAM-P20 was identical to natural SAM-P20. From the results for substrate specificity and inhibitor sensitivity, SAM-P20 could be categorized as a chymotrypsin-like protease with an arginine-cleavable activity, i.e., a serine protease with broad substrate specificity. For proteolytic activity, the optimal pH was 10.0 and the optimal temperature was shifted from 50 to 80 degrees C by the addition of 10 mM calcium ion. The strong stoichiometric inhibition of SAM-P20 activity by SSI dimer protein occurred in a subunit molar ratio of these two proteins of about 1, and an inhibitor constant of SSI toward SAM-P20 was estimated to be 8.0 x 10(-10) M. The complex formation of SAM-P20 and SSI was monitored by analytical gel filtration, and a complex composed of two molecules of SAM-P20 and one dimer molecule of SSI was detected, in addition to a complex of one molecule of SAM-P20 bound to one dimer molecule of SSI. The reactive site of SSI toward SAM-P20 was identified as Met-73-Val-74 by sequence analysis of the modified form of SSI, which was produced by the acidification of the complex of SSI and SAM-P20. This reactive site is the same that toward an exogenous target enzyme, subtilisin BPN'. PMID:7592444

  4. Translating public health knowledge into practice: development of a lay health advisor perinatal tobacco cessation program.

    PubMed

    English, Kevin C; Merzel, Cheryl; Moon-Howard, Joyce

    2010-01-01

    The value of lay health advisor (LHA) interventions as an effective approach toward ameliorating racial, ethnic and/socioeconomic health disparities has been noted by researchers and policy makers. Translating scientific knowledge to bring state-of-the-art health promotion/disease prevention innovation to underserved populations is critical for addressing these health disparities. This article examines the experiences of a community-academic partnership in designing, developing, and implementing an evidence-based, LHA-driven perinatal tobacco cessation program for low-income, predominately African American and Hispanic women. A multimethod process evaluation was conducted to analyze three essential domains of program implementation: (1) fit of the tobacco cessation program into the broader project context, (2) feasibility of program implementation, and (3) fidelity to program implementation protocols. Findings indicate that project partners have largely succeeded in integrating an evidence-based tobacco cessation program into a community-based maternal and infant health project. The successful implementation of this intervention appears to be attributable to the following two predominant factors: (1) the utilization of a scientifically validated tobacco cessation intervention model and (2) the emphasis on continuous LHA training and capacity development. PMID:20357602

  5. MSL/SAM Measurements of Volatile Isotopes, and their Implications for Atmospheric Loss

    NASA Astrophysics Data System (ADS)

    Atreya, Sushil K.; Mahaffy, Paul; Webster, Christopher; Wong, Michael; Conrad, Pamela; Franz, Heather; Grotzinger, John; Jones, John; Leshin, Laurie; Malespin, Charles; Manning, Heidi; Navarro-Gonzalez, Raphael; Owen, Tobias; Pepin, Robert; Schwenzer, Susanne; Trainer, Melissa

    2014-05-01

    High precision measurements of the isotopes of carbon and oxygen in CO2, hydrogen in H2O, nitrogen in N2, and argon in the martian atmosphere have been made by the Sample Analysis at Mars (SAM) instrument on the Curiosity Rover [1,2,3,4]. The resulting values in per mil are 46 for δ13C (relative to VPDB reference standard), 48 for δ18O (VSMOW), 5880 for δD (VSMOW), 572 for δ15N (relative to earth atmosphere), and 4.2 for 36Ar/38Ar (or δ38Ar=310 relative to sun reference standard). The observed enrichment of the heavier isotope over the lighter isotope means that loss to space rather than loss to the surface dominates the isotopic composition in the martian atmosphere. Vertical mixing transports the volatiles from the surface up to the upper atmosphere. While eddy diffusion and molecular diffusion control the distribution of the noble gases, photochemistry also plays a significant role in the distribution of the other volatiles as they diffuse to the upper atmosphere. The above SAM data on the isotopic ratios of carbon, oxygen, hydrogen, nitrogen and argon implies a massive loss of the atmosphere from Mars in the past four billion years. Only hydrogen (hence water) is likely to escape thermally due to the low exospheric temperature of Mars. However, the lack of intrinsic magnetic field on Mars allows solar wind to interact directly with the atmosphere, thus opening up a myriad of possibilities for escape of volatiles from Mars. One such mechanism studied by the ion mass analyzer instrument on Mars Express finds that at current rate of erosion by solar wind, Mars may have lost between 0.2 and 4 millibar of the CO2 atmosphere in the past 3.5 billion years [5]. However, these authors [5] stress that other mechanisms including photochemical, sputtering and cold plasma escape may result in up to 1000 times greater rate of atmospheric loss based on models. Any fractionation in the isotopes of the heavy noble gas, xenon, would have occurred prior to approximately 4 Ga

  6. The nuclear protein Sam68 is recruited to the cytoplasmic stress granules during enterovirus 71 infection.

    PubMed

    Zhang, Hua; Chen, Ning; Li, Pengfei; Pan, Ziye; Ding, Yun; Zou, Dehua; Li, Liyang; Xiao, Lijie; Shen, Binglei; Liu, Shuxia; Cao, Hongwei; Cui, Yudong

    2016-07-01

    Our previous study found that the nuclear protein, 68-kDa Src-associated in mitosis protein (Sam68), is translocated to the cytoplasm and forms punctate pattern during enterovirus 71 (EV71) infection [Virus Research, 180 (2014), 1-11]. However, the exact function of this punctate pattern in cytoplasm during EV71 infection remains unknown. In this study, we firstly have examined this punctate pattern of Sam68 re-localization in the cytoplasm, and observed the obvious recruitments of Sam68 to the EV71-induced stress granules (SGs). Sam68, belongs to the KH domain family of RNA binding proteins (RBPs), was then confirmed that its KH domain was essential for this recruitment. Nevertheless, Knockdown of Sam68 expression using ShRNA had no effects on SGs assembly, indicating that Sam68 is not a constitutive component of the SGs during EV71 infection. Lastly, we investigated the importance of microtubulin transport to SGs aggregation, and revealed that microtubule depolymerization inhibited SGs formation, suggesting that EV71-induced SGs move throughout the cytoplasm in a microtubule-dependent manner. Taken together, these results illuminated that EV71 infections can induce SGs formation, and Sam68, as a SGs component, migrates alone with SGs dependent on intact microtubule upon the viral infections. These findings may provide novel underlying mechanism for delineating the role of SGs during EV71 infection. PMID:27057671

  7. SAMS Acceleration Measurements on Mir from November 1995 to March 1996

    NASA Technical Reports Server (NTRS)

    DeLombard, Richard

    1997-01-01

    The NASA Microgravity Science and Applications Division (MSAD) sponsors science experiments on a variety of microgravity carriers, including Orbiter missions and Russia's Mir space station. The MSAD sponsors the Space Acceleration Measurement System (SAMS) at the NASA Lewis Research Center (LERC) to support these science experiments by providing acceleration measurements to characterize the microgravity environment to which the experiments were exposed. The LeRC Principal Investigator Microgravity Services (PIMS) project supports principal investigations of microgravity science experiments as they evaluate the effects of varying acceleration levels on their experiments. In 1994, a SAMS unit was installed on the Mir space station. In a manner similar to Orbiter mission support, the SAMS unit supports science experiments from the U.S. and Russia by measuring the microgravity environment during experiment operations. Previous reports have summarized the SAMS data acquired during the period from September 1994 to November 1995. During the time period from November 1995 to March 1996, the primary SAMS-supported experiment was a Protein Crystal Growth (PCG) experiment. SAMS data were obtained during the PCG operations on Mir in accordance with the requirements specified by the PCG Principal Investigator. Also included in this data are mission events of interest, such as the undocking of STS-74 from Mir (November 1995) and the docking of Atlantis (STS-76) to Mir in March 1996. This report presents an overview of the SAMS data recorded in the interval from November 1995 to March 1996.

  8. Nothing to declare: mandatory and voluntary disclosure leads advisors to avoid conflicts of interest.

    PubMed

    Sah, Sunita; Loewenstein, George

    2014-02-01

    Professionals face conflicts of interest when they have a personal interest in giving biased advice. Mandatory disclosure--informing consumers of the conflict--is a widely adopted strategy in numerous professions, such as medicine, finance, and accounting. Prior research has shown, however, that such disclosures have little impact on consumer behavior, and can backfire by leading advisors to give even more biased advice. We present results from three experiments with real monetary stakes. These results show that, although disclosure has generally been found to be ineffective for dealing with unavoidable conflicts of interest, it can be beneficial when providers have the ability to avoid conflicts. Mandatory and voluntary disclosure can deter advisors from accepting conflicts of interest so that they have nothing to disclose except the absence of conflicts. We propose that people are averse to being viewed as biased, and that policies designed to activate reputational and ethical concerns will motivate advisors to avoid conflicts of interest. PMID:24379156

  9. Sam68 Mediates the Activation of Insulin and Leptin Signalling in Breast Cancer Cells

    PubMed Central

    Pérez-Pérez, Antonio; Sánchez-Jiménez, Flora; Vilariño-García, Teresa; de la Cruz, Luis; Virizuela, Juan A.; Sánchez-Margalet, Víctor

    2016-01-01

    Obesity is a well-known risk factor for breast cancer development in postmenopausal women. High insulin and leptin levels seem to have a role modulating the growth of these tumours. Sam68 is an RNA-binding protein with signalling functions that has been found to be overexpressed in breast cancer. Moreover, Sam68 may be recruited to insulin and leptin signalling pathways, mediating its effects on survival, growth and proliferation in different cellular types. We aimed to study the expression of Sam68 and its phosphorylation level upon insulin and leptin stimulation, and the role of Sam68 in the proliferative effect and signalling pathways that are activated by insulin or leptin in human breast adenocarcinoma cells. In the human breast adenocarcinoma cell lines MCF7, MDA-MB-231 and BT-474, Sam68 protein quantity and gene expression were increased upon leptin or insulin stimulation, as it was checked by qPCR and immunoblot. Moreover, both insulin and leptin stimulation promoted an increase in Sam68 tyrosine phosphorylation and negatively regulated its RNA binding capacity. siRNA was used to downregulate Sam68 expression, which resulted in lower proliferative effects of both insulin and leptin, as well as a lower activation of MAPK and PI3K pathways promoted by both hormones. These effects may be partly explained by the decrease in IRS-1 expression by down-regulation of Sam68. These results suggest the participation of Sam68 in both leptin and insulin receptor signaling in human breast cancer cells, mediating the trophic effects of these hormones in proliferation and cellular growth. PMID:27415018

  10. An intercomparison of nitrogen-containing species in Nimbus 7 LIMS and SAMS data

    NASA Technical Reports Server (NTRS)

    Jackman, Charles H.; Guthrie, Paul D.; Kaye, Jack A.

    1987-01-01

    Odd-nitrogen chemistry and transport are analyzed using a two-dimensional model and Nimbus 7 data. The Nimbus 7 data include: measurements of O3, NO2, HNO3, H2O, and temperature by the LIMS instrument; N2O, CH4, and temperature measurements by the SAMS instrument; and O3 data from the SBUV instrument. The characteristics of the two-dimensional model used in the study, a modified Guthrie et al. (1984) model, are described. NO2 and HNO3 are calculated using the two-dimensional model, and the computed data are compared with the LIMS NO2 and HNO3 measurements. The model uncertainties are computed based on the photochemical equilibrium assumption, and the role of stratospheric dynamics in determining NO2 and HNO3 is discussed. It is observed that there is good correlation between the LIMS and model data in the upper stratosphere; however, the data do not correspond in the lower stratosphere. The effect of nitrogen sources, such as lightning, on the stratospheric odd nitrogen distribution is examined.

  11. Denitrification of the polar winter stratosphere - Implications of SAM II cloud formation temperatures

    NASA Technical Reports Server (NTRS)

    Hamill, Patrick; Toon, O. B.

    1990-01-01

    The SAM II extinction profiles and the associated temperature profiles are used to determine the amount of denitrification of the winter polar stratospheres. Clear evidence of the denitrification process in the Antarctic data is seen. There are indications in the Arctic data that denitrification mechanisms may be at work there also. At the latitudes observed by the SAM II satellite system, denitrification begins before the formation of extensive ice clouds and may be due to sedimentation of nitric acid particles. However, the possibility of dinitrification by type II PSCs at latitudes not observed by SAM II cannot be excluded.

  12. A comparative study of aerosol extinction measurements made by the SAM II and SAGE satellite experiments

    NASA Technical Reports Server (NTRS)

    Yue, G. K.; Mccormick, M. P.; Chu, W. P.

    1984-01-01

    SAM II and SAGE are two satellite experiments designed to measure stratospheric aerosol extinction using the technique of solar occultation or limb extinction. Although each sensor is mounted aboard a different satellite, there are occasions when their measurement locations are nearly coincident, thereby providing opportunities for a measurement comparison. In this paper, the aerosol extinction profiles and daily contour plots for some of these events in 1979 are reported. The comparisons shown in this paper demonstrate that SAM II and SAGE are producing similar aerosol extinction profiles within their measurement errors and that since SAM II has been previously validated, these results show the validity of the SAGE aerosol measurements.

  13. Large scale study on the variation of RF energy absorption in the head & brain regions of adults and children and evaluation of the SAM phantom conservativeness

    NASA Astrophysics Data System (ADS)

    Keshvari, J.; Kivento, M.; Christ, A.; Bit-Babik, G.

    2016-04-01

    This paper presents the results of two computational large scale studies using highly realistic exposure scenarios, MRI based human head and hand models, and two mobile phone models. The objectives are (i) to study the relevance of age when people are exposed to RF by comparing adult and child heads and (ii) to analyze and discuss the conservativeness of the SAM phantom for all age groups. Representative use conditions were simulated using detailed CAD models of two mobile phones operating between 900 MHz and 1950 MHz including configurations with the hand holding the phone, which were not considered in most previous studies. The peak spatial-average specific absorption rate (psSAR) in the head and the pinna tissues is assessed using anatomically accurate head and hand models. The first of the two mentioned studies involved nine head-, four hand- and two phone-models, the second study included six head-, four hand- and three simplified phone-models (over 400 configurations in total). In addition, both studies also evaluated the exposure using the SAM phantom. Results show no systematic differences between psSAR induced in the adult and child heads. The exposure level and its variation for different age groups may be different for particular phones, but no correlation between psSAR and model age was found. The psSAR from all exposure conditions was compared to the corresponding configurations using SAM, which was found to be conservative in the large majority of cases.

  14. A Matter of Alignment: An Organizational Analysis of the Advisor Role in Three Small Urban High Schools

    ERIC Educational Resources Information Center

    Phillippo, Katherine L.

    2009-01-01

    Recent research literature suggests that students benefit from positive relationships with their teachers. Small high schools attempt to formalize expectations for such relationships through a variety of organizational structures, including the advisor role. As advisors, teachers work with a group of students in order to guide and support them.…

  15. 40 CFR 35.4185 - What does my group do with reports our technical advisor prepares for us?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... technical advisor prepares for us? 35.4185 Section 35.4185 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Grants for Technical Assistance Managing Your Tag § 35.4185 What does my group do with reports our technical advisor prepares...

  16. 40 CFR 35.4185 - What does my group do with reports our technical advisor prepares for us?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... technical advisor prepares for us? 35.4185 Section 35.4185 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Grants for Technical Assistance Managing Your Tag § 35.4185 What does my group do with reports our technical advisor prepares...

  17. 40 CFR 35.4185 - What does my group do with reports our technical advisor prepares for us?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... technical advisor prepares for us? 35.4185 Section 35.4185 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Grants for Technical Assistance Managing Your Tag § 35.4185 What does my group do with reports our technical advisor prepares...

  18. 40 CFR 35.4185 - What does my group do with reports our technical advisor prepares for us?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... technical advisor prepares for us? 35.4185 Section 35.4185 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Grants for Technical Assistance Managing Your Tag § 35.4185 What does my group do with reports our technical advisor prepares...

  19. 40 CFR 35.4185 - What does my group do with reports our technical advisor prepares for us?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... technical advisor prepares for us? 35.4185 Section 35.4185 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Grants for Technical Assistance Managing Your Tag § 35.4185 What does my group do with reports our technical advisor prepares...

  20. 76 FR 33376 - Altegris Advisors, L.L.C. and Northern Lights Fund Trust; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-08

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION Altegris Advisors, L.L.C. and Northern Lights Fund Trust; Notice of Application June 1, 2011...: Altegris Advisors, L.L.C. (the ``Adviser'') and Northern Lights Fund Trust (the ``Trust''). DATES:...

  1. 77 FR 66651 - Salient Advisors, L.P. and MarketShares ETF Trust; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-06

    ... COMMISSION Salient Advisors, L.P. and MarketShares ETF Trust; Notice of Application October 31, 2012 AGENCY... an exemption from sections 12(d)(1)(A) and (B) of the Act. Applicants: Salient Advisors, L.P... requests should be received by the Commission by 5:30 p.m. on November 26, 2012, and should be...

  2. Contextualizing Gay-straight Alliances: Student, Advisor, and Structural Factors Related to Positive Youth Development among Members

    ERIC Educational Resources Information Center

    Poteat, V. Paul; Yoshikawa, Hirokazu; Calzo, Jerel P.; Gray, Mary L.; DiGiovanni, Craig D.; Lipkin, Arthur; Mundy-Shephard, Adrienne; Perrotti, Jeff; Scheer, Jillian R.; Shaw, Matthew P.

    2015-01-01

    Gay-straight alliances (GSAs) may promote resilience. Yet, what GSA components predict well-being? Among 146 youth and advisors in 13 GSAs (58% lesbian, gay, bisexual, or questioning; 64% White; 38% received free/reduced-cost lunch), student (demographics, victimization, attendance frequency, leadership, support, control), advisor (years served,…

  3. College Advisor, Student, and Senior Staff Member Perceptions of Academic Advising Modalities and Types: A Community College Case Study

    ERIC Educational Resources Information Center

    Marvin, Daniel C.

    2013-01-01

    Institutions of higher learning, their senior staff members, and advisors are always striving to create advising modalities and utilize matching advising types that work best for the individual student. This qualitative single-case study was conducted to examine perceptions of the students, advisors, and senior staff members of the advising…

  4. How Do Black Male College Students Develop Supportive Relationships with White Faculty Members and Advisors at a Predominately White Institution

    ERIC Educational Resources Information Center

    Franco, Karina Elizabeth

    2012-01-01

    This qualitative study examined how Black male students develop supportive relationships with White faculty members and advisors at a predominately White urban institution in the Southeastern United State. A multiple-case study method was used to explore how Black males define support, what faculty and advisor characteristics attract Black male…

  5. A Survey on Advisor Perceptions of the Academic Advising Program at the University of Alaska Anchorage Anchorage, Alaska

    ERIC Educational Resources Information Center

    Willis-Haslip, Shirlee

    2011-01-01

    This survey research was the basis of the dissertation for the researcher's doctoral program at George Fox University, and was conducted at the University of Alaska Anchorage (UAA). The purpose of this study was to measure the perception of faculty advisors and professional academic advisors in regard to the importance, responsibility for and…

  6. "Maybe the Communication between Us Was Not Enough": Inside a Dysfunctional Advisor/L2 Advisee Relationship

    ERIC Educational Resources Information Center

    Krase, Ethan

    2007-01-01

    Reporting findings from a 5-month qualitative study of an ESL graduate student as she worked on her master's thesis with the assistance of her advisor, this study offers a detailed portrait of the complicated challenges ESL learners and their advisors may face as they attempt to negotiate working relationships with one another. The study examines…

  7. The Relationship of Worldviews of Advisors and Students and Satisfaction with Advising: A Case of Homogenous Group Impact

    ERIC Educational Resources Information Center

    Coll, Jose E.; Zalaquett, Carlos

    2008-01-01

    The purpose of this study was to investigate whether differences in world-views between academic advisors and their advisees (both traditional and non-traditional students) impact students' use of and satisfaction with the advising process. This study surveyed 115 students and 5 advisors from a four-year liberal arts university in southeastern…

  8. The Role of the Graduate-Level Academic Advisor for Military and Student Veterans: An Ethnographic Study

    ERIC Educational Resources Information Center

    Miller, Michelle A.

    2013-01-01

    The role of a graduate-level academic advisor is essential to all levels of higher education. With the introduction of the Post 9/11 GI Bill, there has been an influx of military and student veterans enrolling in postsecondary and graduate-level education programs. The role of the academic advisor has increased significantly with the influx of…

  9. The Impact of Education Support Advisors on the Attendance, Discipline, and Achievement of At-Risk Students

    ERIC Educational Resources Information Center

    Townsend, Cathy J.

    2010-01-01

    This quantitative, causal-comparative study utilized ex post facto data to examine the impact of education support advisors (ESAs) on the discipline, attendance, and achievement of at-risk students in Grades 3-12. Fourteen education support advisors working in 16 elementary and 8 secondary schools of this mid-Atlantic school district consistently…

  10. 77 FR 41464 - IndexIQ Advisors LLC and IndexIQ Active ETF Trust; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-13

    ... COMMISSION IndexIQ Advisors LLC and IndexIQ Active ETF Trust; Notice of Application July 9, 2012. AGENCY... grant relief from certain disclosure requirements. Applicants: IndexIQ Advisors LLC (``Manager'') and IndexIQ Active ETF Trust (``Trust''). Filing Dates: The application was filed on September 9, 2011,...

  11. 77 FR 49839 - IndexIQ Advisors LLC and IndexIQ Active ETF Trust; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-17

    ... COMMISSION IndexIQ Advisors LLC and IndexIQ Active ETF Trust; Notice of Application August 13, 2012. AGENCY... an exemption from sections 12(d)(1)(A) and (B) of the Act. ] APPLICANTS: IndexIQ Advisors LLC (``IndexIQ'') and IndexIQ Active ETF Trust (the ``Trust''). SUMMARY OF APPLICATION: Applicants request...

  12. 3 CFR 13539 - Executive Order 13539 of April 21, 2010. President's Council of Advisors on Science and Technology

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Council of Advisors on Science and Technology 13539 Order 13539 Presidential Documents Executive Orders Executive Order 13539 of April 21, 2010 EO 13539 President's Council of Advisors on Science and Technology... America, and in order to establish an advisory council on science, technology, and innovation, it...

  13. Working Together to Deliver Usable Climate Information to Agricultural Producers and Advisors in the U.S. Corn Belt

    NASA Astrophysics Data System (ADS)

    Prokopy, L. S.; Widhalm, M.

    2012-12-01

    Weather and climate patterns are a driving force behind the success or failure of cropping systems. With U.S. corn and soybean production accounting for nearly one-third of global supplies and contributing $100 billion annually to the national economy, the ability to successfully produce crops under more variable climate conditions is critical. Useful to Usable (U2U): Transforming Climate Variability and Change Information for Cereal Crop Producers is a USDA-funded research and extension project focused on delivering to producers and farm advisors the resources and training they need to more effectively manage variable climate conditions. The U2U team is a diverse and uniquely qualified group of climatologists, crop modelers, agronomists, economists, and social scientists from 10 Midwestern universities and two NOAA Regional Climate Centers. Together, we strive to help producers make better long-term plans on what, when and where to plant and also how to manage crops for maximum yields and minimum environmental damage. Under development are tools that will allow the agricultural community to examine the financial, production, and environmental outcomes of different management options and climate scenarios so farmers can choose strategies that fit their capabilities and acceptable levels of risk. Researchers are currently using existing data and agro-climate models to investigate the impact of climate conditions on key topics such as crop yields, fieldwork opportunities, nitrogen management, and the cost-effectiveness of irrigation and tiling. To ensure relevance and usability of U2U products, our social science team is using a number of techniques including surveys and focus groups to integrate stakeholder interests, needs, and concerns into all aspects of U2U research. It is through this coupling of physical and social science disciplines that we strive to transform existing climate information into actionable knowledge. This presentation will elaborate on U2U

  14. OARE and SAMS on STS-94/MSL-1

    NASA Technical Reports Server (NTRS)

    Moskowitz, Milton; Hrovat, Kenneth; McPherson, Kevin; Tschen, Peter; DeLombard, Richard; Nati, Maurizio

    1998-01-01

    Four microgravity acceleration measurement instruments were included on MSL-1 to measure the accelerations and vibrations to which science experiments were exposed during their operation on the mission. The data were processed and presented to the principal investigators in a variety of formats to aid their assessment of the microgravity environment during their experiment operations. Two accelerometer systems managed by the NASA Lewis Research Center (LeRC) supported the MSL-1 mission: the Orbital Acceleration Research Experiment (OARE), and the Space Acceleration Measurement System (SAMS). In addition, the Microgravity Measurement Assembly (MMA) and the Quasi- Steady Acceleration Measurement (QSAM) system, both sponsored by the Microgravity Research Division, collected acceleration data as a part of the MSL-1 mission. The NIMA was funded and designed by the European Space Agency in the Netherlands (ESA/ESTEC), and the QSAM system was funded and designed by the German Space Agency (DLR). The Principal Investigator Microgravity Services (PIMS) project at the NASA Lewis Research Center (LeRC) supports Principal Investigators (PIs) of the Microgravity science community as they evaluate the effects of acceleration on their experiments. PIMS primary responsibility is to support NASA-sponsored investigators in the area of acceleration data analysis and interpretation. A mission summary report was prepared and published by PIMS in order to furnish interested experiment investigators with a guide for evaluating the acceleration environment during the MSL-1 mission.

  15. Recursive SAM-based band selection for hyperspectral anomaly detection

    NASA Astrophysics Data System (ADS)

    He, Yuanlei; Liu, Daizhi; Yi, Shihua

    2010-10-01

    Band selection has been widely used in hyperspectral image processing for dimension reduction. In this paper, a recursive SAM-based band selection (RSAM-BBS) method is proposed. Once two initial bands are given, RSAM-BBS is performed in a sequential manner, and at each step the band that can best describe the spectral separation of two hyperspectral signatures is added to the bands already selected until the spectral angle reaches its maximum. In order to demonstrate the utility of the proposed band selection method, an anomaly detection algorithm is developed, which first extracts the anomalous target spectrum from the original image using automatic target detection and classification algorithm (ATDCA), followed by maximum spectral screening (MSS) to estimate the background average spectrum, then implements RSAM-BBS to select bands that participate in the subsequent adaptive cosine estimator (ACE) target detection. As shown in the experimental result on the AVIRIS dataset, less than five bands selected by the RSAM-BBS can achieve comparable detection performance using the full bands.

  16. ExoDat Information System at CeSAM

    NASA Astrophysics Data System (ADS)

    Agneray, F.; Moreau, C.; Chabaud, P.; Damiani, C.; Deleuil, M.

    2014-05-01

    CoRoT (Convection Rotation and planetary transits) is a space based mission led by French space agency (CNES) in association with French and international laboratories. One of CoRoT's goal is to detect exoplanets by the transit method. The Exoplanet Database (Exodat) is a VO compliant information system for the CoRoT exoplanet program. The main functions of ExoDat are to provide a source catalog for the observation fields and targets selection; to characterize the CoRoT targets (spectral type, variability , contamination...);and to support follow up programs. ExoDat is built using the AstroNomical Information System (ANIS) developed by the CeSAM (Centre de donneeS Astrophysique de Marseille). It offers download of observation catalogs and additional services like: search, extract and display data by using a combination of criteria, object list, and cone-search interfaces. Web services have been developed to provide easy access for user's softwares and pipelines.

  17. Conformational Heterogeneity of the SAM-I Riboswitch Transcriptional ON State: A Chaperone-like Role for S-adenosylmethionine

    PubMed Central

    Huang, Wei; Kim, Joohyun; Jha, Shantenu; Aboul-ela, Fareed

    2016-01-01

    Riboswitches are promising targets for the design of novel antibiotics and engineering of portable genetic regulatory elements. There is evidence that variability in riboswitch properties allows tuning of expression for genes involved in different stages of biosynthetic pathways by mechanisms that are not currently understood. Here we explore the mechanism for tuning of SAM-I riboswitch folding. Most SAM-I riboswitches function at the transcriptional level by sensing the cognate ligand— S-adenosyl methionine (SAM). SAM-I riboswitches orchestrate the biosynthetic pathways of cysteine, methionine and SAM, etc. We use base pair probability predictions to examine the secondary structure folding landscape of several SAM-I riboswitch sequences. We predict different folding behaviors for different SAM-I riboswitch sequences. We identify several “decoy” base pairing interactions involving 5’ riboswitch residues that can compete with the formation of a P1 helix, a component of the ligand-bound “transcription OFF” state, in the absence of SAM. We hypothesize that blockage of these interactions through SAM contacts contributes to stabilization of the OFF state in the presence of ligand. We also probe folding patterns for a SAM-I riboswitch RNA using constructs with different 3’ truncation points experimentally. Folding was monitored through fluorescence, susceptibility to base-catalyzed cleavage, nuclear magnetic resonance and indirectly through SAM binding. We identify key decision windows at which SAM can affect the folding pathway toward the OFF state. The presence of decoy conformations and differential sensitivities to SAM at different transcript lengths are crucial for SAM-I riboswitches to modulate gene expression in the context of global cellular metabolism. PMID:22425639

  18. Self-organizing map (SOM) of space acceleration measurement system (SAMS) data.

    PubMed

    Sinha, A; Smith, A D

    1999-01-01

    In this paper, space acceleration measurement system (SAMS) data have been classified using self-organizing map (SOM) networks without any supervision; i.e., no a priori knowledge is assumed regarding input patterns belonging to a certain class. Input patterns are created on the basis of power spectral densities of SAMS data. Results for SAMS data from STS-50 and STS-57 missions are presented. Following issues are discussed in details: impact of number of neurons, global ordering of SOM weight vectors, effectiveness of a SOM in data classification, and effects of shifting time windows in the generation of input patterns. The concept of 'cascade of SOM networks' is also developed and tested. It has been found that a SOM network can successfully classify SAMS data obtained during STS-50 and STS-57 missions. PMID:11543426

  19. Introduction to the Thematic Minireview Series on Radical S-Adenosylmethionine (SAM) Enzymes*

    PubMed Central

    Banerjee, Ruma

    2015-01-01

    In the early days, radical enzyme reactions that use S-adenosylmethionine (SAM) coordinated to an Fe-S cluster, which Perry Frey described as a “poor man's coenzyme B12,” were believed to be relatively rare chemical curiosities. Today, bioinformatics analyses have revealed the wide prevalence and sheer numbers of radical SAM enzymes, conferring superfamily status. In this thematic minireview series, the JBC presents six articles on radical SAM enzymes that accomplish wide-ranging chemical transformations. We learn that despite the diversity of the reactions catalyzed, family members share some common structural and mechanistic themes. Still in its infancy, continued explorations promise to be fertile grounds for discoveries that will undoubtedly further broaden our understanding of the catalytic repertoire and deepen our understanding of the chemical strategies used by radical SAM enzymes. PMID:25477525

  20. Self-organizing map (SOM) of space acceleration measurement system (SAMS) data

    NASA Technical Reports Server (NTRS)

    Sinha, A.; Smith, A. D.

    1999-01-01

    In this paper, space acceleration measurement system (SAMS) data have been classified using self-organizing map (SOM) networks without any supervision; i.e., no a priori knowledge is assumed regarding input patterns belonging to a certain class. Input patterns are created on the basis of power spectral densities of SAMS data. Results for SAMS data from STS-50 and STS-57 missions are presented. Following issues are discussed in details: impact of number of neurons, global ordering of SOM weight vectors, effectiveness of a SOM in data classification, and effects of shifting time windows in the generation of input patterns. The concept of 'cascade of SOM networks' is also developed and tested. It has been found that a SOM network can successfully classify SAMS data obtained during STS-50 and STS-57 missions.

  1. Development of and flight results from the Space Acceleration Measurement System (SAMS)

    NASA Technical Reports Server (NTRS)

    Delombard, Richard; Finley, Brian D.; Baugher, Charles R.

    1992-01-01

    Described here is the development of and the flight results from the Space Acceleration Measurement System (SAMS) flight units used in the Orbiter middeck, Spacelab module, and the Orbitercargo bay. The SAMS units are general purpose microgravity accelerometers designed to support a variety of science experiments with microgravity acceleration measurements. A total of six flight units have been fabricated; four for use in the Orbiter middeck and Spacelab module, and two for use in the Orbiter cargo bay. The design of the units is briefly described. The initial two flights of SAMS units on STS-40 (June 1991) and STS-43 (August 1991) resulted in 371 megabytes and 2.6 gigabytes of data respectively. Analytical techniques developed to examine this quantity of acceleration data are described and sample plots of analyzed data are illustrated. Future missions for the SAMS units are listed.

  2. Tandem SAM Domain Structure of Human Caskin1: A Presynaptic, Self-Assembling Scaffold for CASK

    SciTech Connect

    Stafford, Ryan L.; Hinde, Elizabeth; Knight, Mary Jane; Pennella, Mario A.; Ear, Jason; Digman, Michelle A.; Gratton, Enrico; Bowie, James U.

    2012-02-07

    The synaptic scaffolding proteins CASK and Caskin1 are part of the fibrous mesh of proteins that organize the active zones of neural synapses. CASK binds to a region of Caskin1 called the CASK interaction domain (CID). Adjacent to the CID, Caskin1 contains two tandem sterile a motif (SAM) domains. Many SAM domains form polymers so they are good candidates for forming the fibrous structures seen in the active zone. We show here that the SAM domains of Caskin1 form a new type of SAM helical polymer. The Caskin1 polymer interface exhibits a remarkable segregation of charged residues, resulting in a high sensitivity to ionic strength in vitro. The Caskin1 polymers can be decorated with CASK proteins, illustrating how these proteins may work together to organize the cytomatrix in active zones.

  3. Tandem SAM Domain Structure of Human Caskin1: A Presynaptic, Self-Assembling Scaffold for CASK

    PubMed Central

    Stafford, Ryan L.; Hinde, Elizabeth; Knight, Mary Jane; Pennella, Mario A.; Ear, Jason; Digman, Michelle A.; Gratton, Enrico; Bowie, James U.

    2011-01-01

    Summary The synaptic scaffolding proteins CASK and Caskin1 are part of the fibrous mesh of proteins that organize the active zones of neural synapses. CASK binds to a region of Caskin1 called the CASK interaction domain (CID). Adjacent to the CID, Caskin1 contains two tandem sterile alpha motif (SAM) domains. Many SAM domains form polymers so they are good candidates for forming the fibrous structures seen in the active zone. We show here that the SAM domains of Caskin1 form a new type of SAM helical polymer. The Caskin1 polymer interface exhibits a remarkable segregation of charge residues, resulting in a high sensitivity to ionic strength in vitro. The Caskin1 polymers can be decorated with CASK proteins, illustrating how these proteins may work together to organize the cytomatrix in active zones. PMID:22153505

  4. SAmBA: an interactive software for optimizing the design of biological macromolecules crystallization experiments.

    PubMed

    Audic, S; Lopez, F; Claverie, J M; Poirot, O; Abergel, C

    1997-10-01

    SAmBA is a new software for the design of minimal experimental protocols using the notion of orthogonal arrays of strength 2. The main application of SAmBA is the search of protein crystallization conditions. Given a user input defining the relevant effectors/variables (e.g., pH, temperature, salts) and states (e.g., pH: 5, 6, 7 and 8), this software proposes an optimal set of experiments in which all tested variables and the pairwise interactions between them are symmetrically sampled. No a priori restrictions on the number and range of experimental variables is imposed. SAmBA consists of two complementary programs, SAm and BA, using a simulated annealing approach and a backtracking algorithm, respectively. The software is freely available as C code or as an interactive JAVA applet at http:/(/)igs-server.cnrs-mrs.fr. PMID:9329089

  5. Radical SAM enzymes involved in the biosynthesis of purine-based natural products

    PubMed Central

    Bandarian, Vahe

    2013-01-01

    The radical S-adenosyl-L-methionine (SAM) superfamily is a widely distributed group of iron-sulfur containing proteins that exploit the reactivity of the high energy intermediate, 5’-deoxyadenosyl radical, which is produced by reductive cleavage of SAM, to carry-out complex radical-mediated transformations. The reactions catalyzed by radical SAM enzymes range from simple group migrations to complex reactions in protein and RNA modification. This review will highlight three radical SAM enzymes that catalyze reactions involving modified guanosines in the biosynthesis pathways of the hypermodified tRNA base wybutosine; secondary metabolites of 7-deazapurine structure, including the hypermodified tRNA base queuosine; and the redox cofactor F420. PMID:22902275

  6. Radical SAM enzymes in the biosynthesis of sugar-containing natural products.

    PubMed

    Ruszczycky, Mark W; Ogasawara, Yasushi; Liu, Hung-Wen

    2012-11-01

    Carbohydrates play a key role in the biological activity of numerous natural products. In many instances their biosynthesis requires radical mediated rearrangements, some of which are catalyzed by radical SAM enzymes. BtrN is one such enzyme responsible for the dehydrogenation of a secondary alcohol in the biosynthesis of 2-deoxystreptamine. DesII is another example that catalyzes a deamination reaction necessary for the net C4 deoxygenation of a glucose derivative en route to desosamine formation. BtrN and DesII represent the two most extensively characterized radical SAM enzymes involved in carbohydrate biosynthesis. In this review, we summarize the biosynthetic roles of these two enzymes, their mechanisms of catalysis, the questions that have arisen during these investigations and the insight they can offer for furthering our understanding of radical SAM enzymology. This article is part of a Special Issue entitled: Radical SAM enzymes and Radical Enzymology. PMID:22172915

  7. Radical SAM enzymes in the biosynthesis of sugar-containing natural products☆

    PubMed Central

    Ruszczycky, Mark W.; Ogasawara, Yasushi; Liu, Hung-wen

    2012-01-01

    Carbohydrates play a key role in the biological activity of numerous natural products. In many instances their biosynthesis requires radical mediated rearrangements, some of which are catalyzed by radical SAM enzymes. BtrN is one such enzyme responsible for the dehydrogenation of a secondary alcohol in the biosynthesis of 2-deoxystreptamine. DesII is another example that catalyzes a deamination reaction necessary for the net C4 deoxygenation of a glucose derivative en route to desosamine formation. BtrN and DesII represent the two most extensively characterized radical SAM enzymes involved in carbohydrate biosynthesis. In this review, we summarize the biosynthetic roles of these two enzymes, their mechanisms of catalysis, the questions that have arisen during these investigations and the insight they can offer for furthering our understanding of radical SAM enzymology. This article is part of a Special Issue entitled: Radical SAM enzymes and Radical Enzymology. PMID:22172915

  8. Intercomparison of nitrogen-containing species in Nimbus 7 LIMS and SAMS Data

    SciTech Connect

    Jackman, C.H.; Guthrie, P.D.; Kaye, J.A.

    1987-01-20

    Nitrogen dioxide (NO/sub 2/) and nitric acid (HNO/sub 3/) distributions were derived with a two-dimensional time-dependent model using N/sub 2/O, CH/sub 4/, and temperature measurements from the stratospheric and mesospheric sounder (SAMS) along with H/sub 2/O measurements from the limb infrared monitor of the stratosphere (LIMS) and O/sub 3/ measurements from the solar backscatter ultraviolet (SBUV) instrument. All three instruments (SAMS, LIMS, and SBUV) were aboard the Nimbus 7 satellite. The computed NO/sub 2/ and HNO/sub 3/ were compared with LIMS NO/sub 2/ and HNO/sub 3/ measurements. Calculated NO/sub 2/ is lower than the LIMS NO/sub 2/ in much of the lower stratosphere by about a factor of 4 or more. Differences in the upper stratosphere between derived NO/sub 2/ and LIMS NO/sub 2/ are within the photochemical uncertainties of the computation. Derived NO/sub 2/ is much larger than LIMS NO/sub 2/ at high latitudes in the upper stratosphere in December, indicating that dynamics and/or photochemistry in the atmosphere are different from those used in the model. Derived HNO/sub 3/ is in fairly good agreement with LIMS HNO/sub 3/ in the upper stratosphere. Derived and LIMS HNO/sub 3/ gradually become more different through the middle stratosphere and reach a fairly substantial disagreement in the lower stratosphere. The high-latitude maxima observed in LIMS HNO/sub 3/ are present at lower altitudes and at higher concentrations than those derived in the model computations. More significantly, the derived HNO/sub 3/ at polar latitudes is highest in the summer while LIMS HNO/sub 3/ is highest in the winter. Disagreements in the lower stratosphere between the computed and LIMS NO/sub 2/ and HNO/sub 3/ can only be reduced if two changes are made in the model computations: (1) additional lower stratospheric sources of odd nitrogen (other than N/sub 2/O+O(/sup 1/D)) are included and (2) a modified chemistry to allow the formation of HNO/sub 3/ at the expense of N/sub 2

  9. Bringing a Chemical Laboratory Named Sam to Mars on the 2011 Curiosity Rover

    NASA Astrophysics Data System (ADS)

    Mahaffy, P. R.; Bleacher, L.; Jones, A.; Atreya, S. K.; Manning, H. L.; Cabane, M.; Webster, C. R.; Sam Team

    2010-12-01

    Introduction: An important goal of upcoming missions to Mars is to understand if life could have developed there. The task of the Sample Analysis at Mars (SAM) suite of instruments [1] and the other Curiosity investigations [2] is to move us steadily toward that goal with an assessment of the habitability of our neighboring planet through a series of chemical and geological measurements. SAM is designed to search for organic compounds and inorganic volatiles and measure isotope ratios. Other instruments on Curiosity will provide elemental analysis and identify minerals. SAM will analyze both atmospheric samples and gases evolved from powdered rocks that may have formed billions of years ago with Curiosity providing access to interesting sites scouted by orbiting cameras and spectrometers. SAM Instrument Suite: SAM’s instruments are a Quadrupole Mass Spectrometer (QMS), a 6-column Gas Chromatograph (GC), and a 2-channel Tunable Laser Spectrometer (TLS). SAM can identify organic compounds in Mars rocks to sub-ppb sensitivity and secure precise isotope ratios for C, H, and O in carbon dioxide and water and measure trace levels of methane and its carbon 13 isotope. The SAM gas processing system consists of valves, heaters, pressure sensors, gas scrubbers and getters, traps, and gas tanks used for calibration or combustion experiments [2]. A variety of calibrant compounds interior and exterior to SAM will allow the science and engineering teams to assess SAM’s performance. SAM has been calibrated and tested in a Mars-like environment. Keeping Educators and the Public Informed: The Education and Public Outreach (EPO) goals of the SAM team are to make this complex chemical laboratory and its data widely available to educators, students, and the public. Formal education activities include developing templates for professional development workshops for educators to teach them about SAM and Curiosity, incorporating data into Mars Student Data Teams, and writing articles

  10. Identification of trans-acting factors regulating SamDC expression in Oryza sativa

    SciTech Connect

    Basu, Supratim; Roychoudhury, Aryadeep; Sengupta, Dibyendu N.

    2014-03-07

    Highlights: • Identification of cis elements responsible for SamDC expression by in silico analysis. • qPCR analysis of SamDC expression to abiotic and biotic stress treatments. • Detection of SamDC regulators using identified cis-elements as probe by EMSA. • Southwestern Blot analysis to predict the size of the trans-acting factors. - Abstract: Abiotic stress affects the growth and productivity of crop plants; to cope with the adverse environmental conditions, plants have developed efficient defense machinery comprising of antioxidants like phenolics and flavonoids, and osmolytes like polyamines. SamDC is a key enzyme in the polyamine biosynthesis pathway in plants. In our present communication we have done in silico analysis of the promoter region of SamDC to look for the presence of different cis-regulatory elements contributing to its expression. Based on the presence of different cis-regulatory elements we completed comparative analysis of SamDC gene expression in rice lamina of IR-29 and Nonabokra by qPCR in response to the abiotic stress treatments of salinity, drought, cold and the biotic stress treatments of ABA and light. Additionally, to explore the role of the cis-regulatory elements in regulating the expression of SamDC gene in plants we comparatively analyzed the binding of rice nuclear proteins prepared from IR-29 and Nonabokra undergoing various stress treatments. The intensity of the complex formed was low and inducible in IR-29 in contrast to Nonabokra. Southwestern blot analysis helped in predicting the size of the trans-acting factors binding to these cis-elements. To our knowledge this is the first report on the comprehensive analysis of SamDC gene expression in rice and identification of the trans-acting factors regulating its expression.

  11. Nanoparticle-based solution deposition of gold films supporting bioresistant SAMs.

    PubMed

    Kowalczyk, Bartlomiej; Byrska, Marta; Mahmud, Goher; Huda, Sabil; Kandere-Grzybowska, Kristiana; Grzybowski, Bartosz A

    2009-02-17

    Thin films of gold on glass are prepared by solution deposition of functionalized gold nanoparticles followed by thermal treatment. The processed films adhere strongly to glass without any adhesion layers and can be micropatterned/microetched without delamination from the substrate. The formation of self-assembled monolayers (SAMs) of oligo(ethylene glycol) alkane thiols (EG SAMs) renders the films resistant to cell adhesion and allows for cell patterning. PMID:19170541

  12. The Combustion Experiment on the Sample Analysis at Mars (SAM) Instrument Suite on the Curiosity Rover

    NASA Technical Reports Server (NTRS)

    Stern, J. C.; Malespin, C. A.; Eigenbrode, J. L.; Graham, H. V.; Archer, P. D., Jr.; Brunner, A. E.; Freissinet, C.; Franz, H. B.; Fuentes, J.; Glavin, D. P.; Leshin, L. A.; Mahaffy, P. R.; McAdam, A. C.; Ming, D. W.; Navvaro-Gonzales, R.; Niles, P. B.; Steele, A.

    2014-01-01

    The combustion experiment on the Sample Analysis at Mars (SAM) suite on Curiosity will heat a sample of Mars regolith in the presence of oxygen and measure composition of the evolved gases using quadrupole mass spectrometry (QMS) and tunable laser spectrometry (TLS). QMS will enable detection of combustion products such as CO, CO2, NO, and other oxidized species, while TLS will enable precise measurements of the abundance and carbon isotopic composition (delta(sup 13)C) of the evolved CO2 and hydrogen isotopic composition (deltaD) of H2O. SAM will perform a two-step combustion to isolate combustible materials below approx.550 C and above approx.550 C. The combustion experiment on SAM, if properly designed and executed, has the potential to answer multiple questions regarding the origins of volatiles seen thus far in SAM evolved gas analysis (EGA) on Mars. Constraints imposed by SAM and MSL time and power resources, as well as SAM consumables (oxygen gas), will limit the number of SAM combustion experiments, so it is imperative to design an experiment targeting the most pressing science questions. Low temperature combustion experiments will primarily target the quantification of carbon (and nitrogen) contributed by SAM wet chemistry reagants MTBSTFA (N-Methyl-N-tert-butyldimethylsilyltrifluoroacetamide) and DMF (Dimethylformamide), which have been identified in the background of blank and sample runs and may adsorb to the sample while the cup is in the Sample Manipulation System (SMS). In addition, differences between the sample and "blank" may yield information regarding abundance and delta(sup 13)C of bulk (both organic and inorganic) martian carbon. High temperature combustion experiments primarily aim to detect refractory organic matter, if present in Cumberland fines, as well as address the question of quantification and deltaD value of water evolution associated with hydroxyl hydrogen in clay minerals.

  13. Electronic patient self-assessment and management (SAM): a novel framework for cancer survivorship

    PubMed Central

    2010-01-01

    Background We propose a novel framework for management of cancer survivorship: electronic patient Self-Assessment and Management (SAM). SAM is a framework for transfer of information to and from patients in such a way as to increase both the patient's and the health care provider's understanding of the patient's progress, and to help ensure that patient care follows best practice. Methods Patients who participate in the SAM system are contacted by email at regular intervals and asked to complete validated questionnaires online. Patient responses on these questionnaires are then analyzed in order to provide patients with real-time, online information about their progress and to provide them with tailored and standardized medical advice. Patient-level data from the questionnaires are ported in real time to the patient's health care provider to be uploaded to clinic notes. An initial version of SAM has been developed at Memorial Sloan-Kettering Cancer Center (MSKCC) and the University of California, San Francisco (UCSF) for aiding the clinical management of patients after surgery for prostate cancer. Results Pilot testing at MSKCC and UCSF suggests that implementation of SAM systems are feasible, with no major problems with compliance (> 70% response rate) or security. Conclusion SAM is a conceptually simple framework for passing information to and from patients in such a way as to increase both the patient's and the health care provider's understanding of the patient's progress, and to help ensure that patient care follows best practice. PMID:20565745

  14. Non-canonical active site architecture of the radical SAM thiamin pyrimidine synthase

    DOE PAGESBeta

    Fenwick, Michael K.; Mehta, Angad P.; Zhang, Yang; Abdelwahed, Sameh H.; Begley, Tadhg P.; Ealick, Steven E.

    2015-03-27

    Radical S-adenosylmethionine (SAM) enzymes use a [4Fe-4S] cluster to generate a 5'-deoxyadenosyl radical. Canonical radical SAM enzymes are characterized by a β-barrel-like fold and SAM anchors to the differentiated iron of the cluster, which is located near the amino terminus and within the β-barrel, through its amino and carboxylate groups. Here we show that ThiC, the thiamin pyrimidine synthase in plants and bacteria, contains a tethered cluster-binding domain at its carboxy terminus that moves in and out of the active site during catalysis. In contrast to canonical radical SAM enzymes, we predict that SAM anchors to an additional active sitemore » metal through its amino and carboxylate groups. Superimposition of the catalytic domains of ThiC and glutamate mutase shows that these two enzymes share similar active site architectures, thus providing strong evidence for an evolutionary link between the radical SAM and adenosylcobalamin-dependent enzyme superfamilies.« less

  15. Non-canonical active site architecture of the radical SAM thiamin pyrimidine synthase

    NASA Astrophysics Data System (ADS)

    Fenwick, Michael K.; Mehta, Angad P.; Zhang, Yang; Abdelwahed, Sameh H.; Begley, Tadhg P.; Ealick, Steven E.

    2015-03-01

    Radical S-adenosylmethionine (SAM) enzymes use a [4Fe-4S] cluster to generate a 5‧-deoxyadenosyl radical. Canonical radical SAM enzymes are characterized by a β-barrel-like fold and SAM anchors to the differentiated iron of the cluster, which is located near the amino terminus and within the β-barrel, through its amino and carboxylate groups. Here we show that ThiC, the thiamin pyrimidine synthase in plants and bacteria, contains a tethered cluster-binding domain at its carboxy terminus that moves in and out of the active site during catalysis. In contrast to canonical radical SAM enzymes, we predict that SAM anchors to an additional active site metal through its amino and carboxylate groups. Superimposition of the catalytic domains of ThiC and glutamate mutase shows that these two enzymes share similar active site architectures, thus providing strong evidence for an evolutionary link between the radical SAM and adenosylcobalamin-dependent enzyme superfamilies.

  16. Alternative function for the mitochondrial SAM complex in biogenesis of alpha-helical TOM proteins.

    PubMed

    Stojanovski, Diana; Guiard, Bernard; Kozjak-Pavlovic, Vera; Pfanner, Nikolaus; Meisinger, Chris

    2007-12-01

    The mitochondrial outer membrane contains two preprotein translocases: the general translocase of outer membrane (TOM) and the beta-barrel-specific sorting and assembly machinery (SAM). TOM functions as the central entry gate for nuclear-encoded proteins. The channel-forming Tom40 is a beta-barrel protein, whereas all Tom receptors and small Tom proteins are membrane anchored by a transmembrane alpha-helical segment in their N- or C-terminal portion. Synthesis of Tom precursors takes place in the cytosol, and their import occurs via preexisting TOM complexes. The precursor of Tom40 is then transferred to SAM for membrane insertion and assembly. Unexpectedly, we find that the biogenesis of alpha-helical Tom proteins with a membrane anchor in the C-terminal portion is SAM dependent. Each SAM protein is necessary for efficient membrane integration of the receptor Tom22, whereas assembly of the small Tom proteins depends on Sam37. Thus, the substrate specificity of SAM is not restricted to beta-barrel proteins but also includes the majority of alpha-helical Tom proteins. PMID:18039934

  17. Perceptions and Practices of Technology Student Association Advisors on Implementation Strategies and Teaching Methods

    ERIC Educational Resources Information Center

    Haynie, W. J.; DeLuca, V. W.; Matthews, B.

    2005-01-01

    A study conducted in 1989 surveyed Technology Student Association (TSA) advisors to find their perceptions concerning characteristics of technology education programs with a TSA component and the relationship between participation in co-curricular organizations and the teaching methods used by TSA technology teachers (DeLuca & Haynie, 1991).…

  18. 76 FR 20385 - President's Council of Advisors on Science and Technology; Notice of Meeting: Partially Closed...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-12

    ...This notice sets forth the schedule and summary agenda for a partially closed meeting of the President's Council of Advisors on Science and Technology (PCAST), and describes the functions of the Council. Notice of this meeting is required under the Federal Advisory Committee Act (FACA), 5 U.S.C.,...

  19. Community health advisors' perceptions of the 2005 Dietary Guidelines and MyPyramid

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Abstract Objectives: To describe Community Health Advisors' (CHAs) awareness, knowledge, and perceptions of the 2005 Dietary Guidelines for Americans (2005 DG) and MyPyramid. Methods & Subjects: A 33-item self-administered questionnaire was completed by 106 CHAs residing in Alabama and Mississippi. ...

  20. A Community Health Advisor Program to Reduce Cardiovascular Risk among Rural African-American Women

    ERIC Educational Resources Information Center

    Cornell, C. E.; Littleton, M. A.; Greene, P. G.; Pulley, L.; Brownstein, J. N.; Sanderson, B. K.; Stalker, V. G.; Matson-Koffman, D.; Struempler, B.; Raczynski, J. M.

    2009-01-01

    The Uniontown, Alabama Community Health Project trained and facilitated Community Health Advisors (CHAs) in conducting a theory-based intervention designed to reduce the risk for cardiovascular disease (CVD) among rural African-American women. The multiphased project included formative evaluation and community organization, CHA recruitment and…

  1. Accommodations in Teacher Education: Perspectives of Teacher Candidates with Learning Disabilities and Their Faculty Advisors

    ERIC Educational Resources Information Center

    Csoli, Karen; Gallagher, Tiffany L.

    2012-01-01

    This study of teacher candidates with learning disabilities profiles their experience in a teacher education program. Two teacher candidates and their faculty advisors offer perspectives at various points during the teacher education program. Findings indicate that the teacher candidates were able to complete their required courses when their…

  2. Architects, Captains, and Dreamers: Creating Advisor Roles that Foster Youth-Adult Partnerships

    ERIC Educational Resources Information Center

    Mitra, Dana; Lewis, Tiffanie; Sanders, Felicia

    2013-01-01

    While research has documented the many ways in which student voice can enable educational change, the process of how adults can help to enable student voice is less clear. This article examines how adults new to working as advisors of student voice initiatives begin to develop partnerships with young people. Using a Youth-Adult Partnership…

  3. 78 FR 42115 - NGAM Advisors, LP, et al.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-15

    ... COMMISSION NGAM Advisors, LP, et al.; Notice of Application July 9, 2013. AGENCY: Securities and Exchange..., 2011, and amended on February 8, 2012, July 16, 2012, December 4, 2012, and May 23, 2013. Hearing or... Commission by 5:30 p.m. on August 5, 2013, and should be accompanied by proof of service on applicants,...

  4. 77 FR 5065 - Preservation Trust Advisors, LLC and Northern Lights Fund Trust; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-01

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION Preservation Trust Advisors, LLC and Northern Lights Fund Trust; Notice of Application January 26...'') and Northern Lights Fund Trust (the ``Trust''). FILING DATES: The application was filed on...

  5. Evaluation of a College Curriculum Advisory Program Utilizing Student Advisors. Final Report.

    ERIC Educational Resources Information Center

    Brown, Coke R.

    This study was designed to evaluate the viability of using students as academic advisors for freshmen students and to document the process of the advising program at Idaho State University. Results show that students advised by students did as well academically and had a lower dropout rate than students advised by faculty. Descriptions of advisor…

  6. Crop advisor perceptions of giant ragweed distribution, herbicide-resistance, and management in the Corn Belt

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Giant ragweed has been increasing as a major weed of row crops in North America. We conducted a web-based survey of Certified Crop Advisors in the Corn Belt to determine the current distribution of giant ragweed, the distribution of herbicide resistant populations, and management and ecological fact...

  7. Bridging Horizons. An Advisor's Guide to FFA Involvement for Members with Disabilities.

    ERIC Educational Resources Information Center

    Ploss, Adrienne J.; Field, William E.; Frick, Martin J.

    This guide is designed to provide Future Farmers of America (FFA) advisors with information to assist them in their efforts to include all youth in FFA, including those with disabilities. It addresses benefits of involving youth with disabilities and federal, state, and local legislation and regulations concerning people with disabilities.…

  8. 17 CFR 3.10 - Registration of futures commission merchants, introducing brokers, commodity trading advisors...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 1 2010-04-01 2010-04-01 false Registration of futures commission merchants, introducing brokers, commodity trading advisors, commodity pool operators and leverage transaction merchants. 3.10 Section 3.10 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION REGISTRATION Registration §...

  9. 76 FR 14379 - Subcommittee Meeting of the Board of Advisors to the President, Naval Postgraduate School

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-16

    ... the following meeting of the Subcommittee Meeting of the Board of Advisors (BOA) to The President... deliberations leading to provisional findings for referral to the BOA to the Presidents of the NPS and the Naval... the NPS BOA contact Ms. Jaye Panza, Designated Federal Officer, Naval Postgraduate School,...

  10. Graduate Students' Perceptions of Their Advisors: Is There Systematic Disadvantage in Mentorship?

    ERIC Educational Resources Information Center

    Noy, Shiri; Ray, Rashawn

    2012-01-01

    We explore how race and gender shape graduate students' perceptions of their advisors. We find evidence that women of color and students in the biological/physical sciences report significantly less support than other groups. Our findings speak to the utility of the intersectionality framework for examining interpersonal relations in higher…

  11. Culturally Competent Training Program: A Key to Training Lay Health Advisors for Promoting Breast Cancer Screening

    ERIC Educational Resources Information Center

    Yu, Mei-yu; Song, Lixin; Seetoo, Amy; Cai, Cuijuan; Smith, Gary; Oakley, Deborah

    2007-01-01

    The lay health advisor (LHA) training program for breast cancer screening was conducted among Chinese-English bilingual trainees residing in Southeast Michigan. Guided by Bandura's Social Learning Theory, the development of the training curriculum followed the health communication process recommended by the National Cancer Institute. Data analysis…

  12. The Advisor as Servant: The Theoretical and Philosophical Relevance of Servant Leadership to Academic Advising

    ERIC Educational Resources Information Center

    McClellan, Jeffrey L.

    2007-01-01

    Servant. This novel actively portrays Greenleaf's concept of servant leadership by describing the extracurricular work of a university professor. Consequently, some scholars have demonstrated the relevance of servant leadership to classroom instruction (Powers & Moore, 2005). However, it was not as an instructor, but as an advisor that the…

  13. 75 FR 33369 - EQ Advisors Trust, et al.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-11

    ... to invest in certain financial instruments. Applicants: EQ Advisors Trust, AXA Premier VIP Trust... future will comply with the terms and conditions of the application. \\2\\ A Fund of Funds may not invest... to permit Funds that invest in Underlying Funds in reliance on section 12(d)(1)(G) of the Act,...

  14. 76 FR 11701 - Amendments to Commodity Pool Operator and Commodity Trading Advisor Regulations Resulting From...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-03

    ... would establish business conduct standards for swap dealers and major swap participants. See 75 FR 80638... and Commodity Trading Advisors: Amendments to Compliance Obligations, 76 FR 7976 (Feb. 11, 2011); and Swap Data Recordkeeping and Reporting Requirements; Proposed Rule, 75 FR 76574 (Dec. 8, 2010). II....

  15. Use of Appreciative Inquiry to Explore the Experiences of Faculty Advisors

    ERIC Educational Resources Information Center

    Myers, Valerie Ann-Marie

    2013-01-01

    Academic advising significantly impacts student achievement, persistence, and retention, yet it continues to be an area of weakness for many institutions. The purpose of this sequential mixed-methods case study was to explore the experiences of faculty advisors to identify strengths and weaknesses in their knowledge and skills. Appreciative…

  16. Three Ways Small-Business Advisors Can Counter the Myths of Manufacturing.

    ERIC Educational Resources Information Center

    Thompson, Sara Murray

    1997-01-01

    Addresses the following myths: manufacturers are all large businesses; services are the wave of the future; all manufacturers use new technologies; manufacturing jobs are low skill; manufacturing is unionized; manufacturers are resistant to change and care only about production. Suggests that advisors can help small businesses by serving as a…

  17. Beyond Course-to-Course: New Information On Program Transfer. The Advisor

    ERIC Educational Resources Information Center

    British Columbia Council on Admissions and Transfer, 2003

    2003-01-01

    "The Advisor" provides important information or "system alerts," in user-friendly format and language, on issues and changes affecting admissions and transfer. This document outlines where in the BC Transfer Guide to locate information on transferring between specific programs at different institutions. Please note this document was published…

  18. WASTE ANALYSIS PLAN REVIEWER ADVISOR - AN INTELLIGENT DATABASE TO ASSIST RCRA PERMIT REVIEWERS

    EPA Science Inventory

    The Waste Analysis Plan Review Advisor (WAPRA) system assists in the review of the Waste Analysis Plan Section of RCRA Part B facility permit applications. pecifically, this program automates two functions of the waste analysis plan review. irst, the system checks all wastes whic...

  19. WASTE ANALYSIS PLAN REVIEW ADVISOR - AN INTELLIGENT DATABASE TO ASSIST RCRA PERMIT REVIEWERS

    EPA Science Inventory

    The Waste Analysis Plan Review Advisor (WAPRA) system assists in the review of the Waste Analysis Plan Section of RCRA Part B facility permit applications. Specifically, this program automates two functions of the waste analysis plan review. First, the system checks all wastes wh...

  20. 78 FR 11909 - Emerging Global Advisors, LLC, et al.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-20

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION Emerging Global Advisors, LLC, et al.; Notice of Application February 13, 2013. AGENCY: Securities and Exchange Commission (``Commission''). ACTION: Notice of an application for an order under section 6(c) of the Investment Company Act of...

  1. 75 FR 56055 - Advisory Committee and Species Working Group Technical Advisor Appointments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-15

    ... Atlantic Tunas (ICCAT) as established by the Atlantic Tunas Convention Act of 1975 (ATCA). NMFS is also... Bluefin Tuna Working Group, a Swordfish and Sharks Working Group, a Billfish Working Group, and a BAYS (Bigeye, Albacore, Yellowfin, and Skipjack) Tunas Working Group. Technical Advisors to the species...

  2. Teachers Providing Social and Emotional Support: A Study of Advisor Role Enactment in Small High Schools

    ERIC Educational Resources Information Center

    Phillippo, Kate L.

    2010-01-01

    Background/Context: This study investigates the teacher's role in the student advisory process, which to date has generated limited research literature. Teachers who serve as student advisors assume a role that extends beyond the more traditional instructional role, and includes implied or explicit expectations to provide student advisees with…

  3. The Advisory Working Alliance Inventory--Advisor Version: Scale Development and Validation

    ERIC Educational Resources Information Center

    Schlosser, Lewis Z.; Gelso, Charles J.

    2005-01-01

    The development of the Advisory Working Alliance Inventory-Advisor Version (AWAI-A) is presented. In the first study, data from 236 faculty members from APA-accredited counseling psychology programs were subjected to a principal components analysis, yielding 3 subscales: Rapport (15 items), Apprenticeship (8 items), and Task Focus (8 items). The…

  4. A National Study Examining the Impact of SEVIS on International Student and Scholar Advisors

    ERIC Educational Resources Information Center

    Rosser, Vicki J.; Hermsen, Jill M.; Mamiseishvili, Ketevan; Wood, Melinda S.

    2007-01-01

    The purpose of this research is to examine the impact that Student and Exchange Visitor Information System (SEVIS) is having on the professional worklives of international student and scholar advisors (ISSAs) on U.S. college and university campuses. ISSAs are clearly satisfied with their work and hold their institutions in high regard. However,…

  5. 77 FR 74837 - President's Council of Advisors on Science and Technology (PCAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-18

    ...This notice sets forth the schedule and summary agenda for a partially closed meeting of the President's Council of Advisors on Science and Technology (PCAST), and describes the functions of the Council. Notice of this meeting is required under the Federal Advisory Committee Act (FACA), 5 U.S.C., App....

  6. 75 FR 17912 - Sunshine Act; Notice of Virtual Public Forum for EAC Board of Advisors

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-08

    ... COMMISSION Sunshine Act; Notice of Virtual Public Forum for EAC Board of Advisors Date & Time: Monday, April... conduct business in an efficient manner in a public forum, including being able to review and comment on... take any votes or propose any resolutions during the 5-day forum of April 26-April 30, 2010....

  7. 75 FR 4054 - Sunshine Act; Notice of Virtual Public Forum for EAC Board of Advisors

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-26

    ... COMMISSION Sunshine Act; Notice of Virtual Public Forum for EAC Board of Advisors DATE & TIME: Monday... efficient manner in a public forum, including being able to review and discuss draft documents, when it is... any resolutions during the 12-day forum of February 8-February 19, 2010. Members will post...

  8. "Pleasantly Unexpected": The Nature and Impact of Resident Advisors' Functional Relationships with Faculty

    ERIC Educational Resources Information Center

    Benjamin, Mimi; Griffin, Kimberly A.

    2013-01-01

    Interacting with faculty has several benefits for students; however, little research has explored faculty-student interactions that are neither academic nor social, but occur within the context of students' work. This interpretive study reviews experiences of 73 resident advisors whose work responsibilities require interaction with faculty.…

  9. The Influence of the CAS Standards on Academic Advisors and Advising Programs

    ERIC Educational Resources Information Center

    Keeling, Sarah

    2010-01-01

    Research on the influence of the Council for the Advancement of Standards in Higher Education (CAS) on academic advising is limited. Using a comparative case study method, I respond to this research gap by exploring how the standards influence practices of academic advising programs. Study results indicate that participating advisors knew little…

  10. Modules and Information Retrieval Facilities of the Human Use Regulatory Affairs Advisor (HURAA)

    ERIC Educational Resources Information Center

    Graesser, Arthur C.; Person, Natalie K.; Jackson, G. Tanner; Toth, Jozsef A.

    2004-01-01

    The Human Use Regulatory Affairs Advisor (HURAA) is an Internet facility that provides help and training on the ethical use of human subjects in research, based on documents and regulations in United States Federal agencies. HURAA has a number of standard features of conventional web facilities and computer-based training, such as hypertext,…

  11. MySci Advisors: Establishing a Peer-Mentoring Program for First Year Science Student Support

    ERIC Educational Resources Information Center

    Poling, Kirsten

    2015-01-01

    Would you like to help your students adjust to university life? Perhaps you are simply interested in allowing them to feel more integrated into a department right from the start of their first year? These were the types of issues that we were hoping to address when we founded the MySci Advisors Program, a peer-mentoring group for first year…

  12. Installation and evaluation of a nuclear power plant operator advisor based on artificial intelligence technology

    SciTech Connect

    Hajek, B.K.; Miller, D.W.

    1989-06-20

    This report discusses the following topics on a Nuclear Power Plant operator advisor based on artificial Intelligence Technology; Workstation conversion; Software Conversion; V V Program Development Development; Simulator Interface Development; Knowledge Base Expansion; Dynamic Testing; Database Conversion; Installation at the Perry Simulator; Evaluation of Operator Interaction; Design of Man-Machine Interface; and Design of Maintenance Facility.

  13. 77 FR 38277 - President's Council of Advisors on Science and Technology Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-27

    ...This notice sets forth the schedule and summary agenda for a partially closed meeting of the President's Council of Advisors on Science and Technology (PCAST), and describes the functions of the Council. Notice of this meeting is required under the Federal Advisory Committee Act (FACA), 5 U.S.C.,...

  14. 77 FR 25210 - Advisors Series Trust and Orinda Asset Management, LLC; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-27

    ... reasons discussed below. 6. Applicants assert that the shareholders expect the Advisor subject to the... materially amend subadvisory agreements without shareholder approval and would grant relief from certain... will have been approved by each respective Fund's initial shareholder and the Trust's Board of...

  15. 76 FR 55368 - President's Board of Advisors on Historically Black Colleges and Universities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-07

    ... From the Federal Register Online via the Government Publishing Office DEARTMENT OF EDUCATION President's Board of Advisors on Historically Black Colleges and Universities AGENCY: U.S. Department of... assist the nation in reaching its goal of having the highest proportion of college graduates by 2020;...

  16. 76 FR 7550 - President's Board of Advisors on Historically Black Colleges and Universities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-10

    ... From the Federal Register Online via the Government Publishing Office DEARTMENT OF EDUCATION President's Board of Advisors on Historically Black Colleges and Universities AGENCY: U.S. Department of... institutions that can assist the nation in reaching its goal of having the highest proportion of...

  17. 77 FR 54571 - President's Board of Advisors on Historically Black Colleges and Universities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-05

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF EDUCATION President's Board of Advisors on Historically Black Colleges and Universities AGENCY: President's Board of... institutions that can assist the nation in reaching its goal of having the highest proportion of...

  18. 75 FR 62612 - President's Council of Advisors on Science and Technology Meeting; Notice of Meeting: Partially...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-12

    ... TECHNOLOGY POLICY President's Council of Advisors on Science and Technology Meeting; Notice of Meeting... defense or foreign policy under 5 U.S.C. 552b(c)(1). The precise date and time of this potential meeting has not yet been determined. Public Comments: It is the policy of the PCAST to accept written...

  19. 77 FR 18798 - President's Council of Advisors on Science and Technology (PCAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-28

    ...This notice sets forth the schedule and summary agenda for an open conference call of the President's Council of Advisors on Science and Technology (PCAST), and describes the functions of the Council. Notice of this meeting is required under the Federal Advisory Committee Act (FACA), Public Law 92-463; 86 Stat. 770. The purpose of this conference call is to discuss PCAST's Advanced......

  20. Characteristics of and Challenges for Advisors within a Privatized Extension System

    ERIC Educational Resources Information Center

    Knuth, Ulrike; Knierim, Andrea

    2013-01-01

    Purpose: The aim of this article is to provide evidence on the consequences of the privatization process in Brandenburg with a focus on the agricultural advisors' situation. Before the background of future European Union (EU) expectations on Farm Advisory Systems (FAS), their capacities and competences to respond to such challenges are discussed.…

  1. Modules and Information Retrieval Facilities of the Human Use Regulatory Affairs Advisor (HURAA).

    ERIC Educational Resources Information Center

    Graesser, Arthur C.; Hu, Xiangen; Person, Natalie; Jackson, Tanner; Toth, Joe

    The Human Use Regulatory Affairs Advisor (HURAA) is a Web-based facility that provides help and training on the ethical use of human subjects in research, based on documents and regulations in United States Federal agencies. HURAA has a number of standard features of conventional Web facilities and computer-based training, such as hypertext,…

  2. 78 FR 37605 - Sigma Investment Advisors, LLC, et al.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-21

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION Sigma Investment Advisors, LLC, et al.; Notice of Application June 14, 2013. AGENCY: Securities and Exchange Commission (``Commission''). ACTION: Notice of an application for an order under section 6(c) of the Investment Company Act of 1940...

  3. 77 FR 65593 - PNC Capital Advisors, LLC, et al.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-29

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION PNC Capital Advisors, LLC, et al.; Notice of Application October 23, 2012. AGENCY: Securities and Exchange Commission (``Commission''). ACTION: Notice of an application for an order under section 12(d)(1)(J) of the Investment Company Act...

  4. The Moderating Effect of Personality Traits on Advisor Relationships in Predicting Doctoral Student Burnout

    ERIC Educational Resources Information Center

    Kosh, Emily P.

    2014-01-01

    Personality affects relationships. During the doctoral education, the second most important factor in degree completion, after financial support, is the student-advisor relationship. Approximately half of doctoral students do not finish their degrees. While it is known mentors have a profound impact on the success of doctoral students, the effect…

  5. 78 FR 45459 - Extension of Sunset Date for Attorney Advisor Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-29

    ... and issue decisions that are fully favorable when the documentary record warrants. 72 FR 44763. We... without change as a final rule. 73 FR 11349. Under this rule, some attorney advisors may develop claims... final rule that extended the sunset date of the program until August 10, 2011. 74 FR 33327. We...

  6. Expanding Research in Academic Advising: Methodological Strategies to Engage Advisors in Research

    ERIC Educational Resources Information Center

    Aiken-Wisniewski, Sharon A.; Smith, Joshua S.; Troxel, Wendy G.

    2010-01-01

    Research in academic advising has traditionally been conducted and disseminated by faculty researchers, graduate students, and higher education administrators (including advising directors). While significant in developing a body of literature to guide academic advising, the sources of the contribution also suggest that the frontline advisor does…

  7. Active Region Soft X-Ray Spectra and Temperature Analyses based on Sounding Rocket Measurements from the Solar Aspect Monitor (SAM), - a Modified SDO/EVE Instrument

    NASA Astrophysics Data System (ADS)

    Didkovsky, Leonid V.; Wieman, Seth; Woods, Thomas N.; Jones, Andrew; Moore, Christopher

    2016-05-01

    Some initial results of soft x-ray spectral (0.5 to 3.0 nm) observations of active regions (AR11877 and AR11875) from a sounding rocket flight NASA 36.290 on 21 October 2013 at about 18:30 UT are reported. These observations were made by a Solar Aspect Monitor (SAM), a rocket version of the EUV Variability Experiment’s (EVE) channel, a pinhole camera modified for EVE rocket suite of instruments to include a free-standing transmission grating (200 nm period), which provided spectrally-resolved images of the solar disk. Intensity ratios for strong emission lines extracted from temporally averaged SAM spectral profiles of the ARs were compared to appropriately convolved modeled CHIANTI spectra. These ratios represent the AR’s temperature structures, which are compared to the structures derived from some other observations and temperature models.

  8. Student goal orientation in learning inquiry skills with modifiable software advisors

    NASA Astrophysics Data System (ADS)

    Shimoda, Todd Adrian

    A computer support environment (SCI-WISE) for learning and doing inquiry was designed. The system incorporates software advisors that give task completion advice (eg., forming hypotheses), general purpose advice (e.g., brainstorming), or system modification advice. Advisors' knowledge includes concepts, strategies, examples, referrals to other advisors, and criteria for evaluating good products and skills. Students using SCI-WISE can select the advisors' advice type (specific, general, or hints), and when advisors give advice (anytime, alert, or ask). Students can also modify the advisors' knowledge. The system is designed partly on a theoretical framework that assumes giving students higher levels of agency will facilitate higher-level goal orientation (such as knowledge-building) and produce higher levels of competence. In two studies of sixth graders, science students took a pretest of an open-ended inquiry question and a questionnaire that measured their goal orientations. The students worked in pairs on an inquiry project about memory, using one of two versions of SCI-WISE, one modifiable and one not modifiable. After finishing the project, the students took a posttest similar to the pretest, and evaluated the system. The main hypotheses predicted that knowledge-oriented students using the modifiable version would rate the system higher, use it more effectively, and do better on the inquiry posttest than task-oriented students. The results supported many of the hypotheses generated from the theoretical framework. Knowledge-oriented students tended to rate SCI-WISE higher, use more general purpose and system development advisors, and select more general advice and hints than task-oriented students. On the posttest inquiry test, students with higher goal orientations scored higher on average, particularly when paired with another knowledge oriented student. The studies also showed that goal orientation was not correlated with grade point average. Finally, the

  9. A Role for the GSG Domain in Localizing Sam68 to Novel Nuclear Structures in Cancer Cell Lines

    PubMed Central

    Chen, Taiping; Boisvert, François-Michel; Bazett-Jones, David P.; Richard, Stéphane

    1999-01-01

    The GSG (GRP33, Sam68, GLD-1) domain is a protein module found in an expanding family of RNA-binding proteins. The numerous missense mutations identified genetically in the GSG domain support its physiological role. Although the exact function of the GSG domain is not known, it has been shown to be required for RNA binding and oligomerization. Here it is shown that the Sam68 GSG domain plays a role in protein localization. We show that Sam68 concentrates into novel nuclear structures that are predominantly found in transformed cells. These Sam68 nuclear bodies (SNBs) are distinct from coiled bodies, gems, and promyelocytic nuclear bodies. Electron microscopic studies show that SNBs are distinct structures that are enriched in phosphorus and nitrogen, indicating the presence of nucleic acids. A GFP-Sam68 fusion protein had a similar localization as endogenous Sam68 in HeLa cells, diffusely nuclear with two to five SNBs. Two other GSG proteins, the Sam68-like mammalian proteins SLM-1 and SLM-2, colocalized with endogenous Sam68 in SNBs. Different GSG domain missense mutations were investigated for Sam68 protein localization. Six separate classes of cellular patterns were obtained, including exclusive SNB localization and association with microtubules. These findings demonstrate that the GSG domain is involved in protein localization and define a new compartment for Sam68, SLM-1, and SLM-2 in cancer cell lines. PMID:10473643

  10. SAM-Like Evolved Gas Analyses of Phyllosilicate Minerals and Applications to SAM Analyses of the Sheepbed Mudstone, Gale Crater, Mars

    NASA Technical Reports Server (NTRS)

    McAdam, A. C.; Franz, H. B.; Mahaffy, P. R.; Eigenbrode, J. L.; Stern, J. C.; Brunner, B.; Sutter, B.; Archer, P. D.; Ming , D. W.; Morris, R. V.; Bish, D. L.; Atreya, S. K.

    2014-01-01

    While in Yellowknife Bay, the Mars Science Laboratory Curiosity rover collected two drilled samples, John Klein (hereafter "JK") and Cumberland ("CB"), from the Sheepbed mudstone, as well as a scooped sample from the Rocknest aeolian bedform ("RN"). These samples were sieved by Curiosity's sample processing system and then several subsamples of these materials were delivered to the Sample Analysis at Mars (SAM) instrument suite and the CheMin X-ray diffraction/X-ray fluorescence instrument. CheMin provided the first in situ X-ray diffraction-based evidence of clay minerals on Mars, which are likely trioctahedral smectites (e.g., Fe-saponite) and comprise 20 wt% of the mudstone samples [1]. SAM's evolved gas analysis (EGA) mass spectrometry analyses of JK and CB subsamples, as well as RN subsamples, detected H2O, CO2, O2, H2, SO2, H2S, HCl, NO, OCS, CS2 and other trace gases evolved during pyrolysis. The identity of evolved gases and temperature( s) of evolution can augment mineral detection by CheMin and place constraints on trace volatile-bearing phases present below the CheMin detection limit or those phases difficult to characterize with XRD (e.g., X-ray amorphous phases). Here we will focus on the SAM H2O data, in the context of CheMin analyses, and comparisons to laboratory SAM-like analyses of several phyllosilicate minerals including smectites.

  11. Probing the nature and resistance of the molecule-electrode contact in SAM-based junctions

    NASA Astrophysics Data System (ADS)

    Suchand Sangeeth, C. S.; Wan, Albert; Nijhuis, Christian A.

    2015-07-01

    It is challenging to quantify the contact resistance and to determine the nature of the molecule-electrode contacts in molecular two-terminal junctions. Here we show that potentiodynamic and temperature dependent impedance measurements give insights into the nature of the SAM-electrode interface and other bottlenecks of charge transport (the capacitance of the SAM (CSAM) and the resistance of the SAM (RSAM)), unlike DC methods, independently of each other. We found that the resistance of the top-electrode-SAM contact for junctions with the form of AgTS-SCn//GaOx/EGaIn with n = 10, 12, 14, 16 or 18 is bias and temperature independent and hence Ohmic (non-rectifying) in nature, and is orders of magnitude smaller than RSAM. The CSAM and RSAM are independent of the temperature, indicating that the mechanism of charge transport in these SAM-based junctions is coherent tunneling and the charge carrier trapping at the interfaces is negligible.It is challenging to quantify the contact resistance and to determine the nature of the molecule-electrode contacts in molecular two-terminal junctions. Here we show that potentiodynamic and temperature dependent impedance measurements give insights into the nature of the SAM-electrode interface and other bottlenecks of charge transport (the capacitance of the SAM (CSAM) and the resistance of the SAM (RSAM)), unlike DC methods, independently of each other. We found that the resistance of the top-electrode-SAM contact for junctions with the form of AgTS-SCn//GaOx/EGaIn with n = 10, 12, 14, 16 or 18 is bias and temperature independent and hence Ohmic (non-rectifying) in nature, and is orders of magnitude smaller than RSAM. The CSAM and RSAM are independent of the temperature, indicating that the mechanism of charge transport in these SAM-based junctions is coherent tunneling and the charge carrier trapping at the interfaces is negligible. Electronic supplementary information (ESI) available: Detailed experimental procedure, Nyquist

  12. Fluorocarbon Contamination from the Drill on the Mars Science Laboratory: Potential Science Impact on Detecting Martian Organics by Sample Analysis at Mars (SAM)

    NASA Technical Reports Server (NTRS)

    Eigenbrode, J. L.; McAdam, A.; Franz, H.; Freissinet, C.; Bower, H.; Floyd, M.; Conrad, P.; Mahaffy, P.; Feldman, J.; Hurowitz, J.; Evans, J.; Anderson, M.; Jandura, L.; Brown, K.; Logan, C.; Kuhn, S.; Anderson, R.; Beegle, L.; Limonadi, D.; Rainen, R.; Umland, J.

    2013-01-01

    Polytetrafluoroethylene (PTFE or trade name: Teflon by Dupont Co.) has been detected in rocks drilled during terrestrial testing of the Mars Science Laboratory (MSL) drilling hardware. The PTFE in sediments is a wear product of the seals used in the Drill Bit Assemblies (DBAs). It is expected that the drill assembly on the MSL flight model will also shed Teflon particles into drilled samples. One of the primary goals of the Sample Analysis at Mars (SAM) instrument suite on MSL is to test for the presence of martian organics in samples. Complications introduced by the potential presence of PTFE in drilled samples to the SAM evolved gas analysis (EGA or pyrolysisquadrupole mass spectrometry, pyr-QMS) and pyrolysis- gas chromatography mass spectrometry (Pyr- GCMS) experiments was investigated.

  13. The Search for Nitrates on Mars by the Sample Analysis at Mars (SAM) Instrument

    NASA Technical Reports Server (NTRS)

    Navarro-Gonzalez, Rafael; Stern, Jennifer C.; Freissinet, Caroline; McKay, Chirstopher P.; Sutter, Brad; Archer, P. Douglas, Jr.; McAdam, Amy; Franz, Heather; Coll, Partice J.; Glavin, Daniel Patrick; Eigenbrode, Jennifer L.; Wong, Mike; Atreya, Sushiil K.; Wray, James J.; Steele, Andrew; Prats, Benito D.; Szopa, Cyril; Coscia, David; Teinturier, Samuel; Buch, Arnaud; Leshin, Laurie A.; Ming, Douglas W.; Conrad, Pamela Gales; Cabane, Michel; Mahaffy, Paul R.; Grotzinger, John P.

    2013-01-01

    Planetary models suggest that nitrogen was abundant in the early Martian atmosphere as N2 but it was lost by sputtering and photochemical loss to space, impact erosion, and chemical oxidation to nitrates. A nitrogen cycle may exist on Mars where nitrates, produced early in Mars' history, may have been later decomposed back into N2 by the current impact flux. Nitrates are a fundamental source of nitrogen for terrestrial microorganisms, and they have evolved metabolic pathways to perform both oxidation and reduction to drive a complete biological nitrogen cycle. Therefore, the characterization of nitrogen in Martian soils is important to assess habitability of the Martian environment, particularly with respect to the presence of nitrates. The only previous mission that was designed to search for soil nitrates was the Phoenix mission but N-containing species were not detected by TEGA or the MECA WCL. Nitrates have been tentatively identified in Nakhla meteorites, and if nitrogen was oxidized on Mars, this has important implications for the habitability potential of Mars. Here we report the results from the Sample Analysis at Mars (SAM) instrument suite aboard the Curiosity rover during the first year of surface operations in Gale Crater. Samples from the Rocknest aeolian deposit and sedimentary rocks (John Klein) were heated to approx 835degC under helium flow and the evolved gases were analyzed by MS and GC-MS. Two and possibly three peaks may be associated with the release of m/z 30 at temperatures ranging from 180degC to 500degC. M/z 30 has been tentatively identified as NO; other plausible contributions include CH2O and an isotopologue of CO, 12C18O. NO, CH2O, and CO may be reaction products of reagents (MTBSTFA/DMF) carried from Earth for the wet chemical derivatization experiments with SAM and/or derived from indigenous soil nitrogenated organics. Laboratory analyses indicate that it is also possible that <550degC evolved NO is produced via reaction of HCl with

  14. SAM Sample preparation and its impact on the detection of organic compounds on Mars

    NASA Astrophysics Data System (ADS)

    Buch, Arnaud; Szopa, Cyril; Coll, Patrice; Freissinet, Caroline; Glavin, Daniel; Belmahdi, Imene; François, Pascaline; Millan, Maeva; Eigenbrode, Jennifer; navarro, Rafael; Stern, Jennifer; Pinnick, Veronica; Coscia, David; Teinturier, Samuel; Miller, Kristen; Summons, Roger; Mahaffy, Paul

    2014-05-01

    The wet chemistry experiments on the Sample Analysis at Mars (SAM) [1] experiment in the Curiosity rover of the Mars Science Laboratory mission supports extraction of polar organic compounds from solid samples that improves their detection either by increasing the release of chemical species from solid sample matrices, or by changing their chemical structure to make compounds more amenable to gas chromatography mass spectrometry (GCMS). The wet chemistry approach provides an alternative to the nominal inert-thermal desorption/pyrolysis analytical protocol used by SAM [1] that is more aptly suited for polar components. SAM, includes two different wet chemistry experiments: MTBSTFA derivatization [2-3] and TMAH thermochemolysis [4]. Here we report on the nature of the MTBSTFA derivatization experiment on SAM, the detection of MTBSTFA products in the SAM evolved gas analysis and GCMS experiments, and the implications of this detection. Solid sample were heated up to approximately 840°C at a rate of 35°C/min under He flow. For GC analyses, the majority of the gas released was trapped on a hydrocarbon trap (Tenax®) over a specific temperature range. Adsorbed volatiles on the GC injection trap (IT) were then released into the GC column (CLP-MXT 30m x 0.25mm x 0.25µm) by rapidly heating the IT to 300°C. Then, in order better understand the part of compounds detected coming from internal reaction we have performed several lab experiments to mimic the SAM device. We have investigated the thermal degradation of Tenax®, and possible interaction with MTBSTFA and perchlorate in the SAM trap (Tenax®) to better constrain interpretations of SAM results on Mars. References: [1] Mahaffy, P. et al. (2012) Space Sci Rev, 170, 401-478. [2] Buch, A. et al. (2009) J chrom. A, 43, 143-151. [3] Stalport, F. et al. (2012) Planet. Space Sci. 67: 1-13 [4] Geffroy-Rodier, C. et al. (2009) JAAP, 85, 454-459. Acknowledgements: SAM-GC team acknowledges support from the French Space Agency

  15. Possible Detection of Nitrates on Mars by the Sample Analysis at Mars (SAM) Instrument

    NASA Technical Reports Server (NTRS)

    Navarro-Gonzalez, R.; Stern, J.; Sutter, B.; Archer, D.; McAdam, A.; Franz, H. B.; McKay, C. P.; Coll, P.; Cabane, M.; Ming, D. W.; Brunner, A. E.; Glavin, D.; Eigenbrode, J. L.; Jones, J. H.; Freissinet, C.; Leshin, L.; Wong, M.; Atreya, S.; Wray, J. J.; Steele, A.; Buch, A.; Prats, B. D.; Szopa, C.; Conrad, P.; Mahaffy, P.

    2013-01-01

    Planetary models suggest that nitrogen was abundant in the early Martian atmosphere as dinitrogen (N2). However, it has been lost by sputtering and photochemical loss to space [1, 2], impact erosion [3], and chemical oxidation to nitrates [4]. Nitrates, produced early in Mars history, are later decomposed back into N2 by the current impact flux [5], making possible a nitrogen cycle on Mars. It is estimated that a layer of about 3 m of pure NaNO3 should be distributed globally on Mars [5]. Nitrates are a fundamental source for nitrogen to terrestrial microorganisms. Therefore, the detection of soil nitrates is important to assess habitability in the Martian environment. The only previous mission that was designed to search for soil nitrates was the Phoenix mission but was unable to detect evolved N-containing species by TEGA and the MECA WCL [6]. Nitrates have been tentatively identified in the Nakhla meteorite [7]. The purpose of this work is to determine if nitrates were detected in first solid sample (Rocknest) in Gale Crater examined by the SAM instrument.

  16. Identification of trans-acting factors regulating SamDC expression in Oryza sativa.

    PubMed

    Basu, Supratim; Roychoudhury, Aryadeep; Sengupta, Dibyendu N

    2014-03-01

    Abiotic stress affects the growth and productivity of crop plants; to cope with the adverse environmental conditions, plants have developed efficient defense machinery comprising of antioxidants like phenolics and flavonoids, and osmolytes like polyamines. SamDC is a key enzyme in the polyamine biosynthesis pathway in plants. In our present communication we have done in silico analysis of the promoter region of SamDC to look for the presence of different cis-regulatory elements contributing to its expression. Based on the presence of different cis-regulatory elements we completed comparative analysis of SamDC gene expression in rice lamina of IR-29 and Nonabokra by qPCR in response to the abiotic stress treatments of salinity, drought, cold and the biotic stress treatments of ABA and light. Additionally, to explore the role of the cis-regulatory elements in regulating the expression of SamDC gene in plants we comparatively analyzed the binding of rice nuclear proteins prepared from IR-29 and Nonabokra undergoing various stress treatments. The intensity of the complex formed was low and inducible in IR-29 in contrast to Nonabokra. Southwestern blot analysis helped in predicting the size of the trans-acting factors binding to these cis-elements. To our knowledge this is the first report on the comprehensive analysis of SamDC gene expression in rice and identification of the trans-acting factors regulating its expression. PMID:24530223

  17. Carbon–sulfur bond-forming reaction catalysed by the radical SAM enzyme HydE

    NASA Astrophysics Data System (ADS)

    Rohac, Roman; Amara, Patricia; Benjdia, Alhosna; Martin, Lydie; Ruffié, Pauline; Favier, Adrien; Berteau, Olivier; Mouesca, Jean-Marie; Fontecilla-Camps, Juan C.; Nicolet, Yvain

    2016-05-01

    Carbon–sulfur bond formation at aliphatic positions is a challenging reaction that is performed efficiently by radical S-adenosyl-L-methionine (SAM) enzymes. Here we report that 1,3-thiazolidines can act as ligands and substrates for the radical SAM enzyme HydE, which is involved in the assembly of the active site of [FeFe]-hydrogenase. Using X-ray crystallography, in vitro assays and NMR spectroscopy we identified a radical-based reaction mechanism that is best described as the formation of a C-centred radical that concomitantly attacks the sulfur atom of a thioether. To the best of our knowledge, this is the first example of a radical SAM enzyme that reacts directly on a sulfur atom instead of abstracting a hydrogen atom. Using theoretical calculations based on our high-resolution structures we followed the evolution of the electronic structure from SAM through to the formation of S-adenosyl-L-cysteine. Our results suggest that, at least in this case, the widely proposed and highly reactive 5‧-deoxyadenosyl radical species that triggers the reaction in radical SAM enzymes is not an isolable intermediate.

  18. SAM-FS: LSC's New Solaris-Based Storage Management Product

    NASA Technical Reports Server (NTRS)

    Angell, Kent

    1996-01-01

    SAM-FS is a full featured hierarchical storage management (HSM) device that operates as a file system on Solaris-based machines. The SAM-FS file system provides the user with all of the standard UNIX system utilities and calls, and adds some new commands, i.e. archive, release, stage, sls, sfind, and a family of maintenance commands. The system also offers enhancements such as high performance virtual disk read and write, control of the disk through an extent array, and the ability to dynamically allocate block size. SAM-FS provides 'archive sets' which are groupings of data to be copied to secondary storage. In practice, as soon as a file is written to disk, SAM-FS will make copies onto secondary media. SAM-FS is a scalable storage management system. The system can manage millions of files per system, though this is limited today by the speed of UNIX and its utilities. In the future, a new search algorithm will be implemented that will remove logical and performance restrictions on the number of files managed.

  19. Characterization of a S-adenosyl-l-methionine (SAM)-accumulating strain of Scheffersomyces stipitis.

    PubMed

    Križanović, Stela; Butorac, Ana; Mrvčić, Jasna; Krpan, Maja; Cindrić, Mario; Bačun-Družina, Višnja; Stanzer, Damir

    2015-06-01

    S-adenosyl-l-methionine (SAM) is an important molecule in the cellular metabolism of mammals. In this study, we examined several of the physiological characteristics of a SAM-accumulating strain of the yeast Scheffersomyces stipitis (M12), including SAM production, ergosterol content, and ethanol tolerance. S. stipitis M12 accumulated up to 52.48 mg SAM/g dry cell weight. Proteome analyses showed that the disruption of C-24 methylation in ergosterol biosynthesis, a step mediated by C-24 sterol methyltransferase (Erg6p), results in greater SAM accumulation by S. stipitis M12 compared to the wild-type strain. A comparative proteome-wide analysis identified 25 proteins that were differentially expressed by S. stipitis M12. These proteins are involved in ribosome biogenesis, translation, the stress response, ubiquitin-dependent catabolic processes, the cell cycle, ethanol tolerance, posttranslational modification, peroxisomal membrane stability, epigenetic regulation, the actin cytoskeleton and cell morphology, iron and copper homeostasis, cell signaling, and energy metabolism. PMID:26496619

  20. Oxygen Attachment on Alkanethiolate SAMs Induced by Low-Energy Electron Irradiation

    PubMed Central

    Massey, Sylvain; Bass, Andrew D.; Steffenhagen, Marie; Sanche, Léon

    2013-01-01

    Reactions of 18O2 with self-assembled monolayer (SAM) films of 1-dodecanethiol, 1-octadecanethiol, 1-butanethiol, and benzyl mercaptan chemisorbed on gold, were studied by the electron stimulated desorption (ESD) of anionic fragments over the incident electron energy range 2–20 eV. Dosing the SAMs with 18O2 at 50 K, results in the ESD of 18O− and 18OH−. Electron irradiation of samples prior to 18O2 deposition demonstrates that intensity of subsequent 18O− and 18OH− desorption signals increase with electron fluence and that absent electron pre-irradiation, no 18O− and 18OH− ESD signals are observed, since oxygen is unable to bind to the SAMs. A minimum incident electron energy of 6–7 eV is required to initiate the binding of 18O2 to the SAMs. O2 binding is proposed to proceed by the formation of CHx−1• radicals via resonant dissociative electron attachment and non-resonant C–H dissociation processes. The weaker signals of 18O− and 18OH− from short-chain SAMs are related to the latter’s resistance to electron induced damage, due to the charge-image dipole quenching and electron delocalization. Comparison between the present results and those for DNA oligonucleotides self-assembled on Au [Mirsaleh-Kohan, N. et al. J. Chem. Phys. 2012, 136, 235104] indicates that the oxygen binding mechanism is common to both systems. PMID:23537075

  1. Carbon-sulfur bond-forming reaction catalysed by the radical SAM enzyme HydE.

    PubMed

    Rohac, Roman; Amara, Patricia; Benjdia, Alhosna; Martin, Lydie; Ruffié, Pauline; Favier, Adrien; Berteau, Olivier; Mouesca, Jean-Marie; Fontecilla-Camps, Juan C; Nicolet, Yvain

    2016-05-01

    Carbon-sulfur bond formation at aliphatic positions is a challenging reaction that is performed efficiently by radical S-adenosyl-L-methionine (SAM) enzymes. Here we report that 1,3-thiazolidines can act as ligands and substrates for the radical SAM enzyme HydE, which is involved in the assembly of the active site of [FeFe]-hydrogenase. Using X-ray crystallography, in vitro assays and NMR spectroscopy we identified a radical-based reaction mechanism that is best described as the formation of a C-centred radical that concomitantly attacks the sulfur atom of a thioether. To the best of our knowledge, this is the first example of a radical SAM enzyme that reacts directly on a sulfur atom instead of abstracting a hydrogen atom. Using theoretical calculations based on our high-resolution structures we followed the evolution of the electronic structure from SAM through to the formation of S-adenosyl-L-cysteine. Our results suggest that, at least in this case, the widely proposed and highly reactive 5'-deoxyadenosyl radical species that triggers the reaction in radical SAM enzymes is not an isolable intermediate. PMID:27102684

  2. XPS and ToF-SIMS Investigation of α-Helical and β-Strand Peptide Adsorption onto SAMs

    PubMed Central

    Apte, Julia S.; Collier, Galen; Latour, Robert A.; Gamble, Lara J.; Castner, David G.

    2009-01-01

    14-mer α-helix and a 15-mer β-strand oligopeptides composed of leucine (L) and lysine (K) were used to investigate peptide adsorption and orientation onto well-defined methyl and carboxylic acid terminated self-assembled monolayer (SAM) surfaces with X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). XPS showed both peptides reached monolayer thickness on both SAMs, but significantly higher solution concentrations were required to reach this coverage on the methyl SAMs. This shows that the peptides adsorb more strongly onto the carboxyl-terminated SAMs. The excess oxygen detected by XPS and the H3O+ signal detected by ToF-SIMS for the SAMs with adsorbed peptides indicated that water molecules are associated with the adsorbed peptides, even under ultra-high vacuum conditions. Changes in the amount of L and K fragments detected by ToF-SIMS indicate the β-strand oriented differently on the two SAMs. The L side-chains were preferentially associated with the methyl-terminated SAM and the K side-chains were preferentially associated with the carboxyl SAM. In contrast, little change in the ToF-SIMS K/L ratio was observed for the α-helix peptide absorbed on the two SAMs, indicating ToF-SIMS was not as sensitive to orientation of the α-helix peptide. PMID:19891457

  3. 77 FR 13295 - Subcommittee Meeting of the Board of Advisors to the President of the Naval War College Subcommittee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-06

    ... Committee is: Board of Advisors (BOA) to the Presidents of the Naval Postgraduate School and the Naval War..., information, or to send written comments regarding the NWC BOA Subcommittee contact Mr. Richard R....

  4. 75 FR 53958 - Meeting of the Board of Advisors to the Presidents of the Naval Postgraduate School and the Naval...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-02

    ...), notice is hereby given that the following meeting of the Board of Advisors (BOA) to the Presidents of the... BOA, contact Ms. Jaye Panza, Naval Postgraduate School, 1 University Circle, Monterey, CA...

  5. 78 FR 21349 - Meeting of the Board of Advisors to the Presidents of the Naval Postgraduate School and Naval War...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-10

    ... and Naval War College, Naval Postgraduate School Subcommittee AGENCY: Department of the Navy, DoD... also through the Board of Advisors (BOA) to the Presidents of NPS and Naval War College report...

  6. Nonlinear optical studies of self-assembled monolayers (SAM) silica-SAM-water interface probed with second harmonic generation

    NASA Astrophysics Data System (ADS)

    James, Kenneth Edward

    Second harmonic generation (SHG) is a successful and widely used technique for the study of surfaces and surface phenomena. We present a novel technique using second harmonic generation from oriented water molecules in the Gouy-Chapman diffuse layer at the alkylsiloxane and biomolecular self assembled monolayer (SAM) interface with water to measure distance between the solid surface and the average location of the oriented water in the diffuse layer. This distance is manifest in the SHG angular dependence profile from the relative phases of the second harmonic light generated at the diffuse layer and at the solid surface. Distances of one nanometer can be distinguished. Values for the diffuse layer potential, diffuse layer decay length, magnitude and phase of the nonlinear susceptibility were obtained. This in situ probe is universally applicable for organic adsorbates which in general will push the diffuse layer away from the solid surface. The organic layer thickness can be used to obtain the adsorption fraction. From this and an understanding of the likely chemistry, the orientation of the molecules can be inferred. We have demonstrated this technique on three molecular systems: hydrophobic self assembled monolayers of methoxysilane molecules of varying hydrocarbon chain length, self assembled monolayers of streptavidin glycoproteins and the combined streptavidin-biotinylated antibody monolayer. In the methoxysilane monolayers a relationship between hydrophobicity and molecular orientation was observed. The thickness of the streptavidin monolayer was determined to be 5.6 nm. This is strikingly close to the length of the of the streptavidin molecule which implies a close packed monolayer of streptavidin molecules. The average height of the antibodies was determined to be 10.9 nm or about two thirds the height of an antibody molecule. This too confirms a monolayer and allows for good approximation of surface coverage. This method does nothing to disturb or alter

  7. 17 CFR 240.15Ba1-2 - Registration of municipal advisors and information regarding certain natural persons.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... municipal advisor pursuant to section 15B of the Act (15 U.S.C. 78o-4) must complete Form MA (17 CFR 249... municipal advisor pursuant to section 15B of the Act (15 U.S.C. 78o-4) must complete Form MA-I (17 CFR 249... pursuant to paragraph (a) of this section, must complete Form MA-I (17 CFR 249.1310) in accordance with...

  8. Results From a Lay Health Advisor Intervention to Prevent Lead Poisoning Among Rural Native American Children

    PubMed Central

    Kegler, Michelle Crozier; Malcoe, Lorraine Halinka

    2004-01-01

    Objectives. We tested the effectiveness of a community-based lay health advisor intervention for primary prevention of lead poisoning among Native American children who lived in a former mining area. Methods. We conducted cross-sectional population-based blood lead assessments of Native American and White children aged 1 to 6 years and in-person caregiver interviews before (n=331) and after (n=387) a 2-year intervention. Results. Mean childhood blood lead levels decreased and selected preventive behaviors improved for both Native American and White (comparison) communities. Several short-term outcomes also improved from pre- to postintervention, but only knowledge and hand-washing self-efficacy increased more among Native Americans than among Whites. Conclusions. Our findings provide limited support for the effectiveness of lay health advisor interventions as a primary lead poisoning prevention strategy for Native American communities. PMID:15451742

  9. Development of Fuzzy Logic and Neural Network Control and Advanced Emissions Modeling for Parallel Hybrid Vehicles

    SciTech Connect

    Rajagopalan, A.; Washington, G.; Rizzoni, G.; Guezennec, Y.

    2003-12-01

    This report describes the development of new control strategies and models for Hybrid Electric Vehicles (HEV) by the Ohio State University. The report indicates results from models created in NREL's ADvanced VehIcle SimulatOR (ADVISOR 3.2), and results of a scalable IC Engine model, called in Willan's Line technique, implemented in ADVISOR 3.2.

  10. Recovery Act update from Sr. Advisor Matt Rogers -- End of Obligations

    ScienceCinema

    Rogers, Matt

    2013-05-29

    Senior Advisor to the Secretary for Recovery Act Implementation Matt Rogers shares his thoughts as the Recovery Act reaches a critical milestone -- the end of the 2010 fiscal year and the last day to obligation contract and grant funding under the Recovery Act. For more information about the Recovery Act at the Department of Energy: http://www.energy.gov/recovery Follow the Department of Energy! http://facebook.com/energygov http://twitter.com/energy

  11. Recovery Act update from Sr. Advisor Matt Rogers -- End of Obligations

    SciTech Connect

    Rogers, Matt

    2010-01-01

    Senior Advisor to the Secretary for Recovery Act Implementation Matt Rogers shares his thoughts as the Recovery Act reaches a critical milestone -- the end of the 2010 fiscal year and the last day to obligation contract and grant funding under the Recovery Act. For more information about the Recovery Act at the Department of Energy: http://www.energy.gov/recovery Follow the Department of Energy! http://facebook.com/energygov http://twitter.com/energy

  12. Distributed processing and analysis of physics data in the D0 SAM system at Fermilab

    SciTech Connect

    Igor V. Terekhov

    2001-08-30

    SAM (Sequential Access through Meta-data) is the data access system for the D0 high energy physics (HEP) experiment at Fermilab. The system is being developed and used to handle the Petabyte-scale experiment data. The D0 applications, like virtually all HEP applications, are data-intensive, which poses special problems for the data management and job control facilities in the distributed environment. The fundamental problem is to bring the user applications and the data together, and SAM attacks the problems from both sides. First, we describe how the system moves the data through the distributed disk cache. Second, we describe how SAM interacts with the batch system to synchronize parallel user jobs with the data availability. All the design solutions herein have been implemented in a real system that handles the mission-critical data of the D0 experiment; thus, we present our work from the standpoint of real experience.

  13. Detection of Organics at Mars: How Wet Chemistry Onboard SAM Helps

    NASA Technical Reports Server (NTRS)

    Buch, A.; Freissinet, Caroline; Szopa, C.; Glavin, D.; Coll, P.; Cabane, M.; Eigenbrode, J.; Navarro-Gonzalez, R.; Coscia, D.; Teinturier, S.; Mahaffy, P.

    2013-01-01

    For the first time in the history of space exploration, a mission of interest to astrobiology could be able to analyze refractory organic compounds in the soil of Mars. Wet chemistry experiment allow organic components to be altered in such a way that improves there detection either by releasing the compounds from sample matricies or by changing the chemical structure to be amenable to analytical conditions. The latter is particular important when polar compounds are present. Sample Analysis at Mars (SAM), on the Curiosity rover of the Mars Science Laboratory mission, has onboard two wet chemistry experiments: derivatization and thermochemolysis. Here we report on the nature of the MTBSTFA derivatization experiment on SAM, the detection of MTBSTFA in initial SAM results, and the implications of this detection.

  14. The crystal structure of a novel SAM-dependent methyltransferase PH1915 from Pyrococcus horikoshii.

    SciTech Connect

    Sun, W.; Xu, X.; Pavlova, M.; Edwards, A.; Joachimiak, A.; Savchenko, A.; Christendat, D.; Biosciences Division; Univ. of Toronto; Univ. Health Network

    2005-01-01

    The S-adenosyl-L-methionine (SAM)-dependent methyltransferases represent a diverse and biologically important class of enzymes. These enzymes utilize the ubiquitous methyl donor SAM as a cofactor to methylate proteins, small molecules, lipids, and nucleic acids. Here we present the crystal structure of PH1915 from Pyrococcus horikoshii OT3, a predicted SAM-dependent methyltransferase. This protein belongs to the Cluster of Orthologous Group 1092, and the presented crystal structure is the first representative structure of this protein family. Based on sequence and 3D structure analysis, we have made valuable functional insights that will facilitate further studies for characterizing this group of proteins. Specifically, we propose that PH1915 and its orthologs are rRNA- or tRNA-specific methyltransferases.

  15. SPASM and Twitch Domains in S-Adenosylmethionine (SAM) Radical Enzymes*

    PubMed Central

    Grell, Tsehai A. J.; Goldman, Peter J.; Drennan, Catherine L.

    2015-01-01

    S-Adenosylmethionine (SAM, also known as AdoMet) radical enzymes use SAM and a [4Fe-4S] cluster to catalyze a diverse array of reactions. They adopt a partial triose-phosphate isomerase (TIM) barrel fold with N- and C-terminal extensions that tailor the structure of the enzyme to its specific function. One extension, termed a SPASM domain, binds two auxiliary [4Fe-4S] clusters and is present within peptide-modifying enzymes. The first structure of a SPASM-containing enzyme, anaerobic sulfatase-maturating enzyme (anSME), revealed unexpected similarities to two non-SPASM proteins, butirosin biosynthetic enzyme 2-deoxy-scyllo-inosamine dehydrogenase (BtrN) and molybdenum cofactor biosynthetic enzyme (MoaA). The latter two enzymes bind one auxiliary cluster and exhibit a partial SPASM motif, coined a Twitch domain. Here we review the structure and function of auxiliary cluster domains within the SAM radical enzyme superfamily. PMID:25477505

  16. Perceptions and experiences of using automated bolus advisors amongst people with type 1 diabetes: A longitudinal qualitative investigation

    PubMed Central

    Lawton, J.; Kirkham, J.; Rankin, D.; Barnard, K.; Cooper, C.L.; Taylor, C.; Heller, S.; Elliott, J.

    2014-01-01

    Aims We explored people's reasons for, and experiences of, using bolus advisors to determine insulin doses; and, their likes/dislikes of this technology. Subjects and methods 42 people with type 1 diabetes who had received instruction in use of bolus advisors during a structured education course were interviewed post-course and 6 months later. Data were analysed thematically. Results Participants who considered themselves to have poor mathematical skills highlighted a gratitude for, and heavy reliance on, advisors. Others liked and chose to use advisors because they saved time and effort calculating doses and/or had a data storage facility. Follow-up interviews highlighted that, by virtue of no longer calculating their doses, participants could become deskilled and increasingly dependent on advisors. Some forgot what their mealtime ratios were; others reported a misperception that, because they were pre-programmed during courses, these parameters never needed changing. Use of data storage facilities could hinder effective review of blood glucose data and some participants reported an adverse impact on glycaemic control. Discussion While participants liked and perceived benefits to using advisors, there may be unintended consequences to giving people access to this technology. To promote effective use, on-going input and education from trained health professionals may be necessary. PMID:25451897

  17. Expanding the Use of Time-Based Metering: Multi-Center Traffic Management Advisor

    NASA Technical Reports Server (NTRS)

    Landry, Steven J.; Farley, Todd; Hoang, Ty

    2005-01-01

    Time-based metering is an efficient air traffic management alternative to the more common practice of distance-based metering (or "miles-in-trail spacing"). Despite having demonstrated significant operational benefit to airspace users and service providers, time-based metering is used in the United States for arrivals to just nine airports and is not used at all for non-arrival traffic flows. The Multi-Center Traffic Management Advisor promises to bring time-based metering into the mainstream of air traffic management techniques. Not constrained to operate solely on arrival traffic, Multi-Center Traffic Management Advisor is flexible enough to work in highly congested or heavily partitioned airspace for any and all traffic flows in a region. This broader and more general application of time-based metering is expected to bring the operational benefits of time-based metering to a much wider pool of beneficiaries than is possible with existing technology. It also promises to facilitate more collaborative traffic management on a regional basis. This paper focuses on the operational concept of the Multi-Center Traffic Management Advisor, touching also on its system architecture, field test results, and prospects for near-term deployment to the United States National Airspace System.

  18. Application of artificial intelligence to melter control: Realtime process advisor for the scale melter facility

    SciTech Connect

    Edwards, Jr, R E

    1988-01-01

    The Defense Waste Processing Facility (DWPF) at the Savannah River Plant (SRP) is currently under construction and when completed will process high-level radioactive waste into a borosilicate glass wasteform. This facility will consist of numerous batch chemical processing steps as well as the continuous operation of a joule-heated melter and its off-gas treatment system. A realtime process advisor system based on Artificial Intelligence (AI) techniques has been developed and is currently in use at the semiworks facility, which is operating a 2/3 scale of the DWPF joule-heated melter. The melter advisor system interfaces to the existing data collection and control system and monitors current operations of this facility. The advisor then provides advice to operators and engineers when it identifies process problems. The current system is capable of identifying process problems such as feed system pluggages and thermocouple failures and providing recommended actions. The system also provides facilities normally with distributed control systems. These include the ability to display process flowsheets, monitor alarm conditions, and check the status of process interlocks. 7 figs.

  19. The survey of autobiographical memory (SAM): a novel measure of trait mnemonics in everyday life.

    PubMed

    Palombo, Daniela J; Williams, Lynne J; Abdi, Hervé; Levine, Brian

    2013-06-01

    Compared to the abundance of laboratory-based memory tasks, few measures exist to assess self-reported memory function. This need is particularly important for naturalistic mnemonic capacities, such as autobiographical memory (recall of events and facts from one's past), because it is difficult to reliably assess in the laboratory. Furthermore, naturalistic mnemonic capacities may show stable individual differences that evade the constraints of laboratory testing. The Survey of Autobiographical Memory (SAM) was designed to assess such trait mnemonics, or the dimensional characterization of self-reported mnemonic characteristics. The SAM comprises items assessing self-reported episodic autobiographical, semantic, and spatial memory, as well as future prospection. In a large sample of healthy young adults, the latent dimensional structure of the SAM was characterized with multiple correspondence analysis (MCA). This analysis revealed dimensions corresponding to general mnemonic abilities (i.e., good vs poor memory across subtypes), spatial memory, and future prospection. While episodic and semantic items did not separate in this data-driven analysis, these categories did show expected dissociations in relation to depression history and to laboratory-based measures of recollection. Remote spatial memory as assessed by the SAM showed the expected advantage for males over females. Spatial memory was also related to autobiographical memory performance. Brief versions of the SAM are provided for efficient research applications. Individual differences in memory function are likely related to other health-related factors, including personality, psychopathology, dementia risk, brain structure and function, and genotype. In conjunction with laboratory or performance based assessments, the SAM can provide a useful measure of naturalistic self-report trait mnemonics for probing these relationships. PMID:23063319

  20. Data handling with SAM and art at the NOνA experiment

    NASA Astrophysics Data System (ADS)

    Aurisano, A.; Backhouse, C.; Davies, G. S.; Illingworth, R.; Mayer, N.; Mengel, M.; Norman, A.; Rocco, D.; Zirnstein, J.

    2015-12-01

    During operations, NOvA produces between 5,000 and 7,000 raw files per day with peaks in excess of 12,000. These files must be processed in several stages to produce fully calibrated and reconstructed analysis files. In addition, many simulated neutrino interactions must be produced and processed through the same stages as data. To accommodate the large volume of data and Monte Carlo, production must be possible both on the Fermilab grid and on off-site farms, such as the ones accessible through the Open Science Grid. To handle the challenge of cataloging these files and to facilitate their off-line processing, we have adopted the SAM system developed at Fermilab. SAM indexes files according to metadata, keeps track of each file's physical locations, provides dataset management facilities, and facilitates data transfer to off-site grids. To integrate SAM with Fermilab's art software framework and the NOvA production workflow, we have developed methods to embed metadata into our configuration files, art files, and standalone ROOT files. A module in the art framework propagates the embedded information from configuration files into art files, and from input art files to output art files, allowing us to maintain a complete processing history within our files. Embedding metadata in configuration files also allows configuration files indexed in SAM to be used as inputs to Monte Carlo production jobs. Further, SAM keeps track of the input files used to create each output file. Parentage information enables the construction of self-draining datasets which have become the primary production paradigm used at NOvA. In this paper we will present an overview of SAM at NOvA and how it has transformed the file production framework used by the experiment.