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Sample records for advisor model sam

  1. System Advisor Model, SAM 2014.1.14: General Description

    SciTech Connect

    Blair, N.; Dobos, A. P.; Freeman, J.; Neises, T.; Wagner, M.; Ferguson, T.; Gilman, P.; Janzou, S.

    2014-02-01

    This document describes the capabilities of the U.S. Department of Energy and National Renewable Energy Laboratory's System Advisor Model (SAM), Version 2013.9.20, released on September 9, 2013. SAM is a computer model that calculates performance and financial metrics of renewable energy systems. Project developers, policy makers, equipment manufacturers, and researchers use graphs and tables of SAM results in the process of evaluating financial, technology, and incentive options for renewable energy projects. SAM simulates the performance of photovoltaic, concentrating solar power, solar water heating, wind, geothermal, biomass, and conventional power systems. The financial model can represent financial structures for projects that either buy and sell electricity at retail rates (residential and commercial) or sell electricity at a price determined in a power purchase agreement (utility). SAM's advanced simulation options facilitate parametric and sensitivity analyses, and statistical analysis capabilities are available for Monte Carlo simulation and weather variability (P50/P90) studies. SAM can also read input variables from Microsoft Excel worksheets. For software developers, the SAM software development kit (SDK) makes it possible to use SAM simulation modules in their applications written in C/C++, C#, Java, Python, and MATLAB. NREL provides both SAM and the SDK as free downloads at http://sam.nrel.gov. Technical support and more information about the software are available on the website.

  2. Wind Technology Modeling Within the System Advisor Model (SAM) (Poster)

    SciTech Connect

    Blair, N.; Dobos, A.; Ferguson, T.; Freeman, J.; Gilman, P.; Whitmore, J.

    2014-05-01

    This poster provides detail for implementation and the underlying methodology for modeling wind power generation performance in the National Renewable Energy Laboratory's (NREL's) System Advisor Model (SAM). SAM's wind power model allows users to assess projects involving one or more large or small wind turbines with any of the detailed options for residential, commercial, or utility financing. The model requires information about the wind resource, wind turbine specifications, wind farm layout (if applicable), and costs, and provides analysis to compare the absolute or relative impact of these inputs. SAM is a system performance and economic model designed to facilitate analysis and decision-making for project developers, financers, policymakers, and energy researchers. The user pairs a generation technology with a financing option (residential, commercial, or utility) to calculate the cost of energy over the multi-year project period. Specifically, SAM calculates the value of projects which buy and sell power at retail rates for residential and commercial systems, and also for larger-scale projects which operate through a power purchase agreement (PPA) with a utility. The financial model captures complex financing and rate structures, taxes, and incentives.

  3. System Advisor Model, SAM 2011.12.2: General Description

    SciTech Connect

    Gilman, P.; Dobos, A.

    2012-02-01

    This document describes the capabilities of the U.S. Department of Energy and National Renewable Energy Laboratory's System Advisor Model (SAM), Version 2011.12.2, released on December 2, 2011. SAM is software that models the cost and performance of renewable energy systems. Project developers, policy makers, equipment manufacturers, and researchers use graphs and tables of SAM results in the process of evaluating financial, technology, and incentive options for renewable energy projects. SAM simulates the performance of solar, wind, geothermal, biomass, and conventional power systems. The financial model can represent financing structures for projects that either buy and sell electricity at retail rates (residential and commercial) or sell electricity at a price determined in a power purchase agreement (utility). Advanced analysis options facilitate parametric, sensitivity, and statistical analyses, and allow for interfacing SAM with Microsoft Excel or with other computer programs. SAM is available as a free download at http://sam.nrel.gov. Technical support and more information about the software are available on the website.

  4. Case Studies Comparing System Advisor Model (SAM) Results to Real Performance Data: Preprint

    SciTech Connect

    Blair, N.; Dobos, A.; Sather, N.

    2012-06-01

    NREL has completed a series of detailed case studies comparing the simulations of the System Advisor Model (SAM) and measured performance data or published performance expectations. These case studies compare PV measured performance data with simulated performance data using appropriate weather data. The measured data sets were primarily taken from NREL onsite PV systems and weather monitoring stations.

  5. Parabolic Trough Reference Plant for Cost Modeling with the Solar Advisor Model (SAM)

    SciTech Connect

    Turchi, C.

    2010-07-01

    This report describes a component-based cost model developed for parabolic trough solar power plants. The cost model was developed by the National Renewable Energy Laboratory (NREL), assisted by WorleyParsons Group Inc., for use with NREL's Solar Advisor Model (SAM). This report includes an overview and explanation of the model, two summary contract reports from WorleyParsons, and an Excel spreadsheet for use with SAM. The cost study uses a reference plant with a 100-MWe capacity and six hours of thermal energy storage. Wet-cooling and dry-cooling configurations are considered. The spreadsheet includes capital and operating cost by component to allow users to estimate the impact of changes in component costs.

  6. Molten Salt Power Tower Cost Model for the System Advisor Model (SAM)

    SciTech Connect

    Turchi, C. S.; Heath, G. A.

    2013-02-01

    This report describes a component-based cost model developed for molten-salt power tower solar power plants. The cost model was developed by the National Renewable Energy Laboratory (NREL), using data from several prior studies, including a contracted analysis from WorleyParsons Group, which is included herein as an Appendix. The WorleyParsons' analysis also estimated material composition and mass for the plant to facilitate a life cycle analysis of the molten salt power tower technology. Details of the life cycle assessment have been published elsewhere. The cost model provides a reference plant that interfaces with NREL's System Advisor Model or SAM. The reference plant assumes a nominal 100-MWe (net) power tower running with a nitrate salt heat transfer fluid (HTF). Thermal energy storage is provided by direct storage of the HTF in a two-tank system. The design assumes dry-cooling. The model includes a spreadsheet that interfaces with SAM via the Excel Exchange option in SAM. The spreadsheet allows users to estimate the costs of different-size plants and to take into account changes in commodity prices. This report and the accompanying Excel spreadsheet can be downloaded at https://sam.nrel.gov/cost.

  7. Parabolic Trough Collector Cost Update for the System Advisor Model (SAM)

    SciTech Connect

    Kurup, Parthiv; Turchi, Craig S.

    2015-11-01

    This report updates the baseline cost for parabolic trough solar fields in the United States within NREL's System Advisor Model (SAM). SAM, available at no cost at https://sam.nrel.gov/, is a performance and financial model designed to facilitate decision making for people involved in the renewable energy industry. SAM is the primary tool used by NREL and the U.S. Department of Energy (DOE) for estimating the performance and cost of concentrating solar power (CSP) technologies and projects. The study performed a bottom-up build and cost estimate for two state-of-the-art parabolic trough designs -- the SkyTrough and the Ultimate Trough. The SkyTrough analysis estimated the potential installed cost for a solar field of 1500 SCAs as $170/m2 +/- $6/m2. The investigation found that SkyTrough installed costs were sensitive to factors such as raw aluminum alloy cost and production volume. For example, in the case of the SkyTrough, the installed cost would rise to nearly $210/m2 if the aluminum alloy cost was $1.70/lb instead of $1.03/lb. Accordingly, one must be aware of fluctuations in the relevant commodities markets to track system cost over time. The estimated installed cost for the Ultimate Trough was only slightly higher at $178/m2, which includes an assembly facility of $11.6 million amortized over the required production volume. Considering the size and overall cost of a 700 SCA Ultimate Trough solar field, two parallel production lines in a fully covered assembly facility, each with the specific torque box, module and mirror jigs, would be justified for a full CSP plant.

  8. System Advisor Model

    2010-03-01

    The System Advisor Model (SAM) is a performance and economic model designed to facilitate decision making for people involved in the renewable energy industry, ranging from project managers and engineers to incentive program designers, technology developers, and researchers.

  9. SAM Photovoltaic Model Technical Reference

    SciTech Connect

    Gilman, P.

    2015-05-27

    This manual describes the photovoltaic performance model in the System Advisor Model (SAM). The U.S. Department of Energy’s National Renewable Energy Laboratory maintains and distributes SAM, which is available as a free download from https://sam.nrel.gov. These descriptions are based on SAM 2015.1.30 (SSC 41).

  10. NREL's System Advisor Model Simplifies Complex Energy Analysis (Fact Sheet)

    SciTech Connect

    Not Available

    2015-01-01

    NREL has developed a tool -- the System Advisor Model (SAM) -- that can help decision makers analyze cost, performance, and financing of any size grid-connected solar, wind, or geothermal power project. Manufacturers, engineering and consulting firms, research and development firms, utilities, developers, venture capital firms, and international organizations use SAM for end-to-end analysis that helps determine whether and how to make investments in renewable energy projects.

  11. System Advisor Model: Flat Plate Photovoltaic Performance Modeling Validation Report

    SciTech Connect

    Freeman, J.; Whitmore, J.; Kaffine, L.; Blair, N.; Dobos, A. P.

    2013-12-01

    The System Advisor Model (SAM) is a free software tool that performs detailed analysis of both system performance and system financing for a variety of renewable energy technologies. This report provides detailed validation of the SAM flat plate photovoltaic performance model by comparing SAM-modeled PV system generation data to actual measured production data for nine PV systems ranging from 75 kW to greater than 25 MW in size. The results show strong agreement between SAM predictions and field data, with annualized prediction error below 3% for all fixed tilt cases and below 8% for all one axis tracked cases. The analysis concludes that snow cover and system outages are the primary sources of disagreement, and other deviations resulting from seasonal biases in the irradiation models and one axis tracking issues are discussed in detail.

  12. Generic CSP Performance Model for NREL's System Advisor Model: Preprint

    SciTech Connect

    Wagner, M. J.; Zhu, G.

    2011-08-01

    The suite of concentrating solar power (CSP) modeling tools in NREL's System Advisor Model (SAM) includes technology performance models for parabolic troughs, power towers, and dish-Stirling systems. Each model provides the user with unique capabilities that are catered to typical design considerations seen in each technology. Since the scope of the various models is generally limited to common plant configurations, new CSP technologies, component geometries, and subsystem combinations can be difficult to model directly in the existing SAM technology models. To overcome the limitations imposed by representative CSP technology models, NREL has developed a 'Generic Solar System' (GSS) performance model for use in SAM. This paper discusses the formulation and performance considerations included in this model and verifies the model by comparing its results with more detailed models.

  13. Comparison of Photovoltaic Models in the System Advisor Model: Preprint

    SciTech Connect

    Blair, N. J.; Dobos, A. P.; Gilman, P.

    2013-08-01

    The System Advisor Model (SAM) is free software developed by the National Renewable Energy Laboratory (NREL) for predicting the performance of renewable energy systems and analyzing the financial feasibility of residential, commercial, and utility-scale grid-connected projects. SAM offers several options for predicting the performance of photovoltaic (PV) systems. The model requires that the analyst choose from three PV system models, and depending on that choice, possibly choose from three module and two inverter component models. To obtain meaningful results from SAM, the analyst must be aware of the differences between the model options and their applicability to different modeling scenarios. This paper presents an overview the different PV model options and presents a comparison of results for a 200-kW system using different model options.

  14. Solar Advisor Model User Guide for Version 2.0

    SciTech Connect

    Gilman, P.; Blair, N.; Mehos, M.; Christensen, C.; Janzou, S.; Cameron, C.

    2008-08-01

    The Solar Advisor Model (SAM) provides a consistent framework for analyzing and comparing power system costs and performance across the range of solar technologies and markets, from photovoltaic systems for residential and commercial markets to concentrating solar power and large photovoltaic systems for utility markets. This manual describes Version 2.0 of the software, which can model photovoltaic and concentrating solar power technologies for electric applications for several markets. The current version of the Solar Advisor Model does not model solar heating and lighting technologies.

  15. Technical Manual for the SAM Physical Trough Model

    SciTech Connect

    Wagner, M. J.; Gilman, P.

    2011-06-01

    NREL, in conjunction with Sandia National Lab and the U.S Department of Energy, developed the System Advisor Model (SAM) analysis tool for renewable energy system performance and economic analysis. This paper documents the technical background and engineering formulation for one of SAM's two parabolic trough system models in SAM. The Physical Trough model calculates performance relationships based on physical first principles where possible, allowing the modeler to predict electricity production for a wider range of component geometries than is possible in the Empirical Trough model. This document describes the major parabolic trough plant subsystems in detail including the solar field, power block, thermal storage, piping, auxiliary heating, and control systems. This model makes use of both existing subsystem performance modeling approaches, and new approaches developed specifically for SAM.

  16. Technoeconomic Modeling of Battery Energy Storage in SAM

    SciTech Connect

    DiOrio, Nicholas; Dobos, Aron; Janzou, Steven; Nelson, Austin; Lundstrom, Blake

    2015-09-01

    Detailed comprehensive lead-acid and lithium-ion battery models have been integrated with photovoltaic models in an effort to allow System Advisor Model (SAM) to offer the ability to predict the performance and economic benefit of behind the meter storage. In a system with storage, excess PV energy can be saved until later in the day when PV production has fallen, or until times of peak demand when it is more valuable. Complex dispatch strategies can be developed to leverage storage to reduce energy consumption or power demand based on the utility rate structure. This document describes the details of the battery performance and economic models in SAM.

  17. Technical Manual for the SAM Biomass Power Generation Model

    SciTech Connect

    Jorgenson, J.; Gilman, P.; Dobos, A.

    2011-09-01

    This technical manual provides context for the implementation of the biomass electric power generation performance model in the National Renewable Energy Laboratory's (NREL's) System Advisor Model (SAM). Additionally, the report details the engineering and scientific principles behind the underlying calculations in the model. The framework established in this manual is designed to give users a complete understanding of behind-the-scenes calculations and the results generated.

  18. Detailed Physical Trough Model for NREL's Solar Advisor Model: Preprint

    SciTech Connect

    Wagner, M. J.; Blair, N.; Dobos, A.

    2010-10-01

    Solar Advisor Model (SAM) is a free software package made available by the National Renewable Energy Laboratory (NREL), Sandia National Laboratory, and the US Department of Energy. SAM contains hourly system performance and economic models for concentrating solar power (CSP) systems, photovoltaic, solar hot-water, and generic fuel-use technologies. Versions of SAM prior to 2010 included only the parabolic trough model based on Excelergy. This model uses top-level empirical performance curves to characterize plant behavior, and thus is limited in predictive capability for new technologies or component configurations. To address this and other functionality challenges, a new trough model; derived from physical first principles was commissioned to supplement the Excelergy-based empirical model. This new 'physical model' approaches the task of characterizing the performance of the whole parabolic trough plant by replacing empirical curve-fit relationships with more detailed calculations where practical. The resulting model matches the annual performance of the SAM empirical model (which has been previously verified with plant data) while maintaining run-times compatible with parametric analysis, adding additional flexibility in modeled system configurations, and providing more detailed performance calculations in the solar field, power block, piping, and storage subsystems.

  19. Sensitivity of Concentrating Solar Power Trough Performance, Cost and Financing with Solar Advisor Model

    SciTech Connect

    Blair, N.; Mehos, M.; Christensen, C.

    2008-03-01

    A comprehensive solar technology systems analysis model, the Solar Advisor Model (SAM) was developed to support the federal R&D community and the solar industry. This model, developed by staff at NREL and Sandia National Laboratory, is able to model the costs, finances, and performance of concentrating solar power and photovoltaics (PV). Currently, parabolic troughs and concentrating PV are the two concentrating technologies modeled within the SAM environment.

  20. Reference Manual for the System Advisor Model's Wind Power Performance Model

    SciTech Connect

    Freeman, J.; Jorgenson, J.; Gilman, P.; Ferguson, T.

    2014-08-01

    This manual describes the National Renewable Energy Laboratory's System Advisor Model (SAM) wind power performance model. The model calculates the hourly electrical output of a single wind turbine or of a wind farm. The wind power performance model requires information about the wind resource, wind turbine specifications, wind farm layout (if applicable), and costs. In SAM, the performance model can be coupled to one of the financial models to calculate economic metrics for residential, commercial, or utility-scale wind projects. This manual describes the algorithms used by the wind power performance model, which is available in the SAM user interface and as part of the SAM Simulation Core (SSC) library, and is intended to supplement the user documentation that comes with the software.

  1. Incorporation of NREL Solar Advisor Model Photovoltaic Capabilities with GridLAB-D

    SciTech Connect

    Tuffner, Francis K.; Hammerstrom, Janelle L.; Singh, Ruchi

    2012-10-19

    This report provides a summary of the work updating the photovoltaic model inside GridLAB-D. The National Renewable Energy Laboratory Solar Advisor Model (SAM) was utilized as a basis for algorithms and validation of the new implementation. Subsequent testing revealed that the two implementations are nearly identical in both solar impacts and power output levels. This synergized model aides the system-level impact studies of GridLAB-D, but also allows more specific details of a particular site to be explored via the SAM software.

  2. Spatial Analysis and Modeling Systems (SAMS)

    NASA Technical Reports Server (NTRS)

    Vermillion, Charles; Chan, Paul; Hill, John; Jaske, Robert; Rochon, Gilbert; Stetina, Fran

    1991-01-01

    The objective is to develop a uniform environmental data gathering and distribution system to support (1) emergency management for environmental disasters, and (2) the calibration and validation of remotely sensed data. Initial activities will be to select a data test site and to demonstrate multi-discipline applications using simulated or satellite data in a non real-time mode. Rainfall and flooding are chosen as the testbeds for the SAMS concept because of the abundance of data and the availability of models. The capability to display and process GOES data and analyze GOES generated rain-rate maps will be integrated into SAMS.

  3. Modeling Photovoltaic and Concentrating Solar Power Trough Performance, Cost, and Financing with Solar Advisor Model

    SciTech Connect

    Blair, N.; Mehos, M.; Christensen, C.; Cameron, C.

    2008-01-01

    A comprehensive solar technology systems analysis model, the Solar Advisor Model (SAM), has been developed to support the federal R&D community and the solar industry by staff at the National Renewable Energy Laboratory (NREL) and Sandia National Laboratory. This model is able to model the finances, incentives, and performance of flat-plate photovoltaic (PV), concentrating PV, and concentrating solar power (specifically, parabolic troughs). The primary function of the model is to allow users to investigate the impact of variations in performance, cost, and financial parameters to better understand their impact on key figures of merit. Figures of merit related to the cost and performance of these systems include, but aren't limited to, system output, system efficiencies, levelized cost of energy, return on investment, and system capital and O&M costs. SAM allows users to do complex system modeling with an intuitive graphical user interface (GUI). In fact, all tables and graphics for this paper are taken directly from the model GUI. This model has the capability to compare different solar technologies within the same interface, making use of similar cost and finance assumptions. Additionally, the ability to do parametric and sensitivity analysis is central to this model. There are several models within SAM to model the performance of photovoltaic modules and inverters. This paper presents an overview of each PV and inverter model, introduces a new generic model, and briefly discusses the concentrating solar power (CSP) parabolic trough model. A comparison of results using the different PV and inverter models is also presented.

  4. Integration, Validation, and Application of a PV Snow Coverage Model in SAM

    SciTech Connect

    Ryberg, David; Freeman, Janine

    2015-09-01

    Due to the increasing deployment of PV systems in snowy climates, there is significant interest in a method capable of estimating PV losses resulting from snow coverage that has been verified for a wide variety of system designs and locations. A scattering of independent snow coverage models have been developed over the last 15 years; however, there has been very little effort spent on verifying these models beyond the system design and location on which they were based. Moreover, none of the major PV modeling software products have incorporated any of these models into their workflow. In response to this deficiency, we have integrated the methodology of the snow model developed in the paper by Marion et al. [1] into the National Renewable Energy Laboratory's (NREL) System Advisor Model (SAM). In this work we describe how the snow model is implemented in SAM and discuss our demonstration of the model's effectiveness at reducing error in annual estimations for two PV arrays. Following this, we use this new functionality in conjunction with a long term historical dataset to estimate average snow losses across the United States for a typical PV system design. The open availability of the snow loss estimation capability in SAM to the PV modeling community, coupled with our results of the nation-wide study, will better equip the industry to accurately estimate PV energy production in areas affected by snowfall.

  5. Direct-Steam Linear Fresnel Performance Model for NREL's System Advisor Model

    SciTech Connect

    Wagner, M. J.; Zhu, G.

    2012-09-01

    This paper presents the technical formulation and demonstrated model performance results of a new direct-steam-generation (DSG) model in NREL's System Advisor Model (SAM). The model predicts the annual electricity production of a wide range of system configurations within the DSG Linear Fresnel technology by modeling hourly performance of the plant in detail. The quasi-steady-state formulation allows users to investigate energy and mass flows, operating temperatures, and pressure drops for geometries and solar field configurations of interest. The model includes tools for heat loss calculation using either empirical polynomial heat loss curves as a function of steam temperature, ambient temperature, and wind velocity, or a detailed evacuated tube receiver heat loss model. Thermal losses are evaluated using a computationally efficient nodal approach, where the solar field and headers are discretized into multiple nodes where heat losses, thermal inertia, steam conditions (including pressure, temperature, enthalpy, etc.) are individually evaluated during each time step of the simulation. This paper discusses the mathematical formulation for the solar field model and describes how the solar field is integrated with the other subsystem models, including the power cycle and optional auxiliary fossil system. Model results are also presented to demonstrate plant behavior in the various operating modes.

  6. P50/P90 Analysis for Solar Energy Systems Using the System Advisor Model: Preprint

    SciTech Connect

    Dobos, A. P.; Gilman, P.; Kasberg, M.

    2012-06-01

    To secure competitive financing for a solar energy generation project, the economic risk associated with interannual solar resource variability must be quantified. One way to quantify this risk is to calculate exceedance probabilities representing the amount of energy expected to be produced by a plant. Many years of solar radiation and metereological data are required to determine these values, often called P50 or P90 values for the level of certainty they represent. This paper describes the two methods implemented in the National Renewable Energy Laboratory's System Advisor Model (SAM) to calculate P50 and P90 exceedance probabilities for solar energy projects. The methodology and supporting data sets are applicable to photovoltaic, solar water heating, and concentrating solar power (CSP) systems.

  7. Modeling Photovoltaic Module-Level Power Electronics in the System Advisor Model; NREL (National Renewable Energy Laboratory)

    SciTech Connect

    2015-07-01

    Module-level power electronics, such as DC power optimizers, microinverters, and those found in AC modules, are increasing in popularity in smaller-scale photovoltaic (PV) systems as their prices continue to decline. Therefore, it is important to provide PV modelers with guidelines about how to model these distributed power electronics appropriately in PV modeling software. This paper extends the work completed at NREL that provided recommendations to model the performance of distributed power electronics in NREL’s popular PVWatts calculator [1], to provide similar guidelines for modeling these technologies in NREL's more complex System Advisor Model (SAM). Module-level power electronics - such as DC power optimizers, microinverters, and those found in AC modules-- are increasing in popularity in smaller-scale photovoltaic (PV) systems as their prices continue to decline. Therefore, it is important to provide PV modelers with guidelines about how to model these distributed power electronics appropriately in PV modeling software.

  8. The fSAM Model of False Recall

    ERIC Educational Resources Information Center

    Kimball, Daniel R.; Smith, Troy A.; Kahana, Michael J.

    2007-01-01

    The authors report a new theory of false memory building upon existing associative memory models and implemented in fSAM, the first fully specified quantitative model of false recall. Participants frequently intrude unstudied critical words while recalling lists comprising their strongest semantic associates but infrequently produce other…

  9. Modeling Photovoltaic and Concentrating Solar Power Trough Performance, Cost, and Financing with the Solar Advisor Model: Preprint

    SciTech Connect

    Blair, N.; Mehos, M.; Christensen, C.; Cameron, C.

    2008-05-01

    A comprehensive solar technology systems analysis model, the Solar Advisor Model (SAM), has been developed to support the federal R&D community and the solar industry by staff at the National Renewable Energy Laboratory (NREL) and Sandia National Laboratory. This model is able to model the finances, incentives, and performance of flat-plate photovoltaic (PV), concentrating PV, and concentrating solar power (specifically, parabolic troughs). The primary function of the model is to allow users to investigate the impact of variations in performance, cost, and financial parameters to better understand their impact on key figures of merit. Figures of merit related to the cost and performance of these systems include, but aren't limited to, system output, system efficiencies, levelized cost of energy, return on investment, and system capital and O&M costs. There are several models within SAM to model the performance of photovoltaic modules and inverters. This paper presents an overview of each PV and inverter model, introduces a new generic model, and briefly discusses the concentrating solar power (CSP) parabolic trough model. A comparison of results using the different PV and inverter models is also presented.

  10. Introducing the aerosol-climate model MAECHAM5-SAM2

    NASA Astrophysics Data System (ADS)

    Hommel, R.; Timmreck, C.; Graf, H. F.

    2009-04-01

    We are presenting a new global aerosol model MAECHAM5-SAM2 to study the aerosol dynamics in the UTLS under background and volcanic conditions. The microphysical core modul SAM2 treats the formation, the evolution and the transport of stratospheric sulphuric acid aerosol. The aerosol size distribution and the weight percentage of the sulphuric acid solution is calculated dependent on the concentrations of H2SO4 and H2O, their vapor pressures, the atmospheric temperature and pressure. The fixed sectional method is used to resolve an aerosol distribution between 1 nm and 2.6 micron in particle radius. Homogeneous nucleation, condensation and evaporation, coagulation, water-vapor growth, sedimentation and sulphur chemistry are included. The module is applied in the middle-atmosphere MAECHAM5 model, resolving the atmosphere up to 0.01 hPa (~80 km) in 39 layers. It is shown here that MAECHAM5-SAM2 well represents in-situ measured size distributions of stratospheric background aerosol in the northern hemisphere mid-latitudes. Distinct differences can be seen when derived integrated aerosol parameters (surface area, effective radius) are compared with aerosol climatologies based on the SAGE II satellite instrument (derived by the University of Oxford and the NASA AMES laboratory). The bias between the model and the SAGE II data increases as the moment of the aerosol size distribution decreases. Thus the modeled effective radius show the strongest bias, followed by the aerosol surface area density. Correspondingly less biased are the higher moments volume area density and the mass density of the global stratospheric aerosol coverage. This finding supports the key finding No. 2 of the SPARC Assessment of Stratospheric Aerosol Properties (2006), where it was shown that during periods of very low aerosol load in the stratosphere, the consistency between in-situ and satellite measurements, which exist in a volcanically perturbed stratosphere, breaks down and significant

  11. A Spatial Analysis and Modeling System (SAMS) for environment management

    NASA Technical Reports Server (NTRS)

    Stetina, Fran; Hill, John; Chan, Paul; Jaske, Robert; Rochon, Gilbert

    1993-01-01

    This is a proposal to develop a uniform global environmental data gathering and distribution system to support the calibration and validation of remotely sensed data. SAMS is based on an enhanced version of FEMA's Integrated Emergency Management Information Systems and the Department of Defense's Air land Battlefield Environment Software Systems. This system consists of state-of-the-art graphics and visualization techniques, simulation models, database management and expert systems for conducting environmental and disaster preparedness studies. This software package will be integrated into various Landsat and UNEP-GRID stations which are planned to become direct readout stations during the EOS (Earth Observing System) timeframe. This system would be implemented as a pilot program to support the Tropical Rainfall Measuring Mission (TRMM). This will be a joint NASA-FEMA-University-Industry project.

  12. A Spatial Analysis and Modeling System (SAMS) for environment management

    NASA Technical Reports Server (NTRS)

    Vermillion, Charles H.; Stetina, Fran; Hill, John; Chan, Paul; Jaske, Robert; Rochon, Gilbert

    1992-01-01

    This is a proposal to develop a uniform global environmental data gathering and distribution system to support the calibration and validation of remotely sensed data. SAMS is based on an enhanced version of FE MA's Integrated Emergency Management Information Systems and the Department of Defense's Air Land Battlefield Environment Software Systems. This system consists of state-of-the-art graphics and visualization techniques, simulation models, database management and expert systems for conducting environmental and disaster preparedness studies. This software package will be integrated into various Landsat and UNEP-GRID stations which are planned to become direct readout stations during the EOS timeframe. This system would be implemented as a pilot program to support the Tropical Rainfall Measuring Mission (TRMM). This will be a joint NASA-FEMA-University-Industry project.

  13. Solar Advisor Model; Session: Modeling and Analysis (Presentation)

    SciTech Connect

    Blair, N.

    2008-04-01

    This project supports the Solar America Initiative by: (1) providing a consistent framework for analyzing and comparing power system costs and performance across the range of solar technologies and markets, PV, solar heat systems, CSP, residential, commercial and utility markets; (2) developing and validating performance models to enable accurate calculation of levelized cost of energy (LCOE); (3) providing a consistent modeling platform for all TPP's; and (4) supporting implementation and usage of cost models.

  14. Construct Validation of the Louisiana School Analysis Model (SAM) Instructional Staff Questionnaire

    ERIC Educational Resources Information Center

    Bray-Clark, Nikki; Bates, Reid

    2005-01-01

    The purpose of this study was to validate the Louisiana SAM Instructional Staff Questionnaire, a key component of the Louisiana School Analysis Model. The model was designed as a comprehensive evaluation tool for schools. Principle axis factoring with oblique rotation was used to uncover the underlying structure of the SISQ. (Contains 1 table.)

  15. The SAM framework: modeling the effects of management factors on human behavior in risk analysis.

    PubMed

    Murphy, D M; Paté-Cornell, M E

    1996-08-01

    Complex engineered systems, such as nuclear reactors and chemical plants, have the potential for catastrophic failure with disastrous consequences. In recent years, human and management factors have been recognized as frequent root causes of major failures in such systems. However, classical probabilistic risk analysis (PRA) techniques do not account for the underlying causes of these errors because they focus on the physical system and do not explicitly address the link between components' performance and organizational factors. This paper describes a general approach for addressing the human and management causes of system failure, called the SAM (System-Action-Management) framework. Beginning with a quantitative risk model of the physical system, SAM expands the scope of analysis to incorporate first the decisions and actions of individuals that affect the physical system. SAM then links management factors (incentives, training, policies and procedures, selection criteria, etc.) to those decisions and actions. The focus of this paper is on four quantitative models of action that describe this last relationship. These models address the formation of intentions for action and their execution as a function of the organizational environment. Intention formation is described by three alternative models: a rational model, a bounded rationality model, and a rule-based model. The execution of intentions is then modeled separately. These four models are designed to assess the probabilities of individual actions from the perspective of management, thus reflecting the uncertainties inherent to human behavior. The SAM framework is illustrated for a hypothetical case of hazardous materials transportation. This framework can be used as a tool to increase the safety and reliability of complex technical systems by modifying the organization, rather than, or in addition to, re-designing the physical system.

  16. Evolution of SAM in an enhanced model for Monitoring WLCG services

    NASA Astrophysics Data System (ADS)

    Collados, David; Shade, John; Traylen (Cern, Steve; Imamagic (Srce, Emir

    2010-04-01

    It is four years now since the first prototypes of tools and tests started to monitor the Worldwide LHC Computing Grid (WLCG) services. One of these tools is the Service Availability Monitoring (SAM) framework, which superseded the SFT tool, and has become a keystone for the monthly WLCG availability and reliability computations. During this time, the grid has evolved into a robust, production-level infrastructure, in no small part thanks to the extensive monitoring infrastructure which includes testing, visualization and reporting. Experience gained with monitoring has led to emerging grid monitoring standards, and provided valuable input for the Operations Automation Strategy aimed at the regionalization of monitoring services. This change in scope, together with an ever-increasing number of services and infrastructures, make enhancements in the architecture of existing monitoring tools a necessity. This paper describes the present architecture of SAM, an enhanced and distributed model for monitoring WLCG services, and the required changes in SAM to adopt this new model inside the EGEE-III project.

  17. Major Advisor.

    ERIC Educational Resources Information Center

    Chatwin, Marshall

    This paper describes a computer program, Major Advisor (MA), which helps students identify college majors. Used in conjunction with career counseling and advising, MA provides information to students who are developing their educational plans. The program matches students' personal preferences and the requirements/characteristics of 130 common…

  18. Results and Comparison from the SAM Linear Fresnel Technology Performance Model: Preprint

    SciTech Connect

    Wagner, M. J.

    2012-04-01

    This paper presents the new Linear Fresnel technology performance model in NREL's System Advisor Model. The model predicts the financial and technical performance of direct-steam-generation Linear Fresnel power plants, and can be used to analyze a range of system configurations. This paper presents a brief discussion of the model formulation and motivation, and provides extensive discussion of the model performance and financial results. The Linear Fresnel technology is also compared to other concentrating solar power technologies in both qualitative and quantitative measures. The Linear Fresnel model - developed in conjunction with the Electric Power Research Institute - provides users with the ability to model a variety of solar field layouts, fossil backup configurations, thermal receiver designs, and steam generation conditions. This flexibility aims to encompass current market solutions for the DSG Linear Fresnel technology, which is seeing increasing exposure in fossil plant augmentation and stand-alone power generation applications.

  19. A Case Study Inquiry on Faculty Advisors and the Appreciative Advising Model

    ERIC Educational Resources Information Center

    Finch, Brian S.

    2013-01-01

    Appreciative advising is an innovative academic advising method based on the organizational development theory of appreciative inquiry. This approach emphasizes student strengths through a collaborative relationship between advisor and student using open-ended questions and focusing on positive potential. This study addressed the local problem of…

  20. SAM Technical Review Committee Final Report: Summary and Key Recommendations from the Onsite TRC Meeting Held April 22-23, 2013

    SciTech Connect

    Blair, N.; Dobos, S.; Janzou, S.; Gilman, P.; Freeman, J.; Kaffine, L.

    2013-08-01

    The System Advisor Model (SAM) is a broad and robust set of models and frameworks for analyzing both system performance and system financing. It does this across a range of technologies dominated by solar technologies including photovoltaics (PV) and concentrated solar power (CSP). The U.S. Department of Energy (DOE) Solar Energy Technology Program requested the SAM development team to review the photovoltaic performance modeling with the development community and specifically, with the independent engineering community. The report summarizes the major effort for this technical review committee (TRC).

  1. Senescence-accelerated mouse (SAM): a novel murine model of senescence.

    PubMed

    Takeda, T; Hosokawa, M; Higuchi, K

    1997-01-01

    The Senescence-Accelerated Mouse (SAM) has been under development by our research team at Kyoto University since 1970 through the selective inbreeding of the AKR/J strain of mice donated by the Jackson Laboratory in 1968, based on a graded score for senescence, life span, and pathologic phenotype. At present, there are 12 lines of SAM: nine senescence-prone inbred strains (SAMP) including SAMP1, SAMP2, SAMP3, SAMP6, SAMP7, SAMP8, SAMP9, SAMP10, and SAMP11; and three senescence-resistant inbred strains (SAMR) including SAMR1, SAMR4, and SAMR5. Data from survival curves, Gompertzian function, and grading score of senescence, together with growth patterns of body weight of these SAMP and SAMR, revealed that the characteristic feature of aging common to all SAMP mice is "accelerated senescence;" early onset and irreversible advance of senescence manifested by several signs and gross lesions such as the loss of normal behavior, various skin lesions, increased lordokyphosis, etc., after a period of normal development. In the course of SAM development, it became evident that SAMP strains manifest various pathologic phenotypes that are characteristic enough to differentiate the SAM strains. The genetic background and significance of SAM development are discussed. PMID:9088907

  2. Senescence-accelerated mouse (SAM) with special references to neurodegeneration models, SAMP8 and SAMP10 mice.

    PubMed

    Takeda, Toshio

    2009-04-01

    The SAM strains, a group of related inbred strains consisting of senescence-prone inbred strains (SAMP) and senescence-resistant inbred strains (SAMR), have been successfully developed by selective inbreeding of the AKR/J strain of mice donated by the Jackson laboratory in 1968. The characteristic feature of aging common to the SAMP and SAMR is accelerated senescence and normal aging, respectively. Furthermore, SAMP and SAMR strains of mice manifest various pathobiological phenotypes spontaneously. Among SAMP strains, SAMP8 and SAMP10 mice show age-related behavioral deterioration such as deficits in learning and memory, emotional disorders (reduced anxiety-like behavior and depressive behavior) and altered circadian rhythm associated with certain pathological, biochemical and pharmacological changes. Here, the previous and recent literature on SAM mice are reviewed with an emphasis on SAMP8 and SAMP10 mice. A spontaneous model like SAM with distinct advantages over the gene-modified model is hoped by investigators to be used more widely as a biogerontological resource to explore the etiopathogenesis of accelerated senescence and neurodegenerative disorders. PMID:19199030

  3. Senescence-accelerated mouse (SAM) as an animal model of senile dementia: pharmacological, neurochemical and molecular biological approach.

    PubMed

    Okuma, Y; Nomura, Y

    1998-12-01

    To elucidate the fundamental mechanism of age-related deficiencies of learning and to develop effective drugs for intervention in age-related diseases such as learning dysfunctions, pertinent animal models that have characteristics closely similar to human dysfunctions should be established. SAM (senescence-accelerated mouse) has been established as a murine model of the SAM strains, groups of related inbred strains including nine strains of accelerated senescence-prone, short-lived mice (SAMP) and three strains of accelerated senescence-resistant, long-lived mice (SAMR). SAMP-strain mice show relatively strain-specific age-associated phenotypic pathologies such as shortened life span and early manifestation of senescence. Among the SAMP-strain mice, SAMP8 mice show an age-related deterioration in learning ability. Here, the neuropathological, neurochemical and pharmacological features of SAM are reported, especially for SAMP8. Moreover, the effects of several drugs on the biochemical and behavioral alterations in SAMP8 and the etiologic manifestation of accelerated senescence are also discussed. PMID:9920195

  4. Building Capacity in a Rural North Carolina Community to Address Prostate Health Using a Lay Health Advisor Model.

    PubMed

    Vines, Anissa I; Hunter, Jaimie C; White, Brandolyn S; Richmond, Alan N

    2016-05-01

    Background Prostate cancer is a critical concern for African Americans in North Carolina (NC), and innovative strategies are needed to help rural African American men maximize their prostate health. Engaging the community in research affords opportunities to build capacity for teaching and raising awareness. Approach and Strategies A community steering committee of academicians, community partners, religious leaders, and other stakeholders modified a curriculum on prostate health and screening to include interactive knowledge- and skill-building activities. This curriculum was then used to train 15 African American lay health advisors, dubbed Prostate Cancer Ambassadors, in a rural NC community. Over the 2-day training, Ambassadors achieved statistically significant improvements in knowledge of prostate health and maintained confidence in teaching. The Ambassadors, in turn, used their personal networks to share their knowledge with over 1,000 individuals in their community. Finally, the Ambassadors became researchers, implementing a prostate health survey in local churches. Discussion and Conclusions It is feasible to use community engagement models for raising awareness of prostate health in NC African American communities. Mobilizing community coalitions to develop curricula ensures that the curricula meet the communities' needs, and training lay health advisors to deliver curricula helps secure community buy-in for the information.

  5. Tracking the MSL-SAM methane detection source location Through Mars Regional Atmospheric Modeling System (MRAMS)

    NASA Astrophysics Data System (ADS)

    Pla-García, Jorge

    2016-04-01

    1. Introduction: The putative in situ detection of methane by Sample Analysis at Mars (SAM) instrument suite on Curiosi-ty at Gale crater has garnered significant attention because of the potential implications for the presence of geological methane sources or indigenous Martian organisms [1, 2]. SAM reported detection of back-ground levels of atmospheric methane of mean value 0.69±0.25 parts per billion by volume (ppbv) at the 95% confidence interval (CI). Additionally, in four sequential measurements spanning a 60-sol period, SAM observed elevated levels of methane of 7.2±2.1 ppbv (95% CI), implying that Mars is episodically producing methane from an additional unknown source. There are many major unresolved questions regard-ing this detection: 1) What are the potential sources of the methane release? 2) What causes the rapid decrease in concentration? and 3) Where is the re-lease location? 4) How spatially extensive is the re-lease? 5) For how long is CH4 released? Regarding the first question, the source of methane, is so far not identified. It could be related with geo-logical process like methane release from clathrates [3], serpentinisation [4] and volcanism [5]; or due to biological activity from methanogenesis [6]. To answer the second question, the rapid decrease in concentration, it is important to note that the photo-chemical lifetime of methane is of order 100 years, much longer than the atmospheric mixing time scale, and thus the gas should tend to be well mixed except near a source or shortly after an episodic release. The observed spike of 7 ppb from the background of <1 ppb, and then the rapid return to the background lev-el could be due to a sink (destruction) or due to at-mospheric mixing. A wind mediated erosion process of ordinary quartz crystals was proposed to produce activated quartz grains, which sequester methane by forming covalent Si-C bonds. If this process is op-erational on Mars today, which some recent prelimi-nary studies on

  6. Dosimetric comparison of the specific anthropomorphic mannequin (SAM) to 14 anatomical head models using a novel definition for the mobile phone positioning

    NASA Astrophysics Data System (ADS)

    Kainz, Wolfgang; Christ, Andreas; Kellom, Tocher; Seidman, Seth; Nikoloski, Neviana; Beard, Brian; Kuster, Niels

    2005-07-01

    This paper presents new definitions for obtaining reproducible results in numerical phone dosimetry. Numerous numerical dosimetric studies have been published about the exposure of mobile phone users which concluded with conflicting results. However, many of these studies lack reproducibility due to shortcomings in the description of the phone positioning. The new approach was tested by two groups applying two different numerical program packages to compare the specific anthropomorphic mannequin (SAM) to 14 anatomically correct head models. A novel definition for the positioning of mobile phones next to anatomically correct head models is given along with other essential parameters to be reported. The definition is solely based on anatomical characteristics of the head. A simple up-to-date phone model was used to determine the peak spatial specific absorption rate (SAR) of mobile phones in SAM and in the anatomically correct head models. The results were validated by measurements. The study clearly shows that SAM gives a conservative estimate of the exposure in anatomically correct head models for head only tissue. Depending on frequency, phone position and head size the numerically calculated 10 g averaged SAR in the pinna can be up to 2.1 times greater than the peak spatial SAR in SAM. Measurements in small structures, such as the pinna, will significantly increase the uncertainty; therefore SAM was designed for SAR assessment in the head only. Whether SAM will provide a conservative value for the pinna depends on the pinna SAR limit of the safety standard considered.

  7. Tracking the MSL-SAM methane detection source location Through Mars Regional Atmospheric Modeling System (MRAMS)

    NASA Astrophysics Data System (ADS)

    Pla-García, Jorge

    2016-04-01

    1. Introduction: The putative in situ detection of methane by Sample Analysis at Mars (SAM) instrument suite on Curiosi-ty at Gale crater has garnered significant attention because of the potential implications for the presence of geological methane sources or indigenous Martian organisms [1, 2]. SAM reported detection of back-ground levels of atmospheric methane of mean value 0.69±0.25 parts per billion by volume (ppbv) at the 95% confidence interval (CI). Additionally, in four sequential measurements spanning a 60-sol period, SAM observed elevated levels of methane of 7.2±2.1 ppbv (95% CI), implying that Mars is episodically producing methane from an additional unknown source. There are many major unresolved questions regard-ing this detection: 1) What are the potential sources of the methane release? 2) What causes the rapid decrease in concentration? and 3) Where is the re-lease location? 4) How spatially extensive is the re-lease? 5) For how long is CH4 released? Regarding the first question, the source of methane, is so far not identified. It could be related with geo-logical process like methane release from clathrates [3], serpentinisation [4] and volcanism [5]; or due to biological activity from methanogenesis [6]. To answer the second question, the rapid decrease in concentration, it is important to note that the photo-chemical lifetime of methane is of order 100 years, much longer than the atmospheric mixing time scale, and thus the gas should tend to be well mixed except near a source or shortly after an episodic release. The observed spike of 7 ppb from the background of <1 ppb, and then the rapid return to the background lev-el could be due to a sink (destruction) or due to at-mospheric mixing. A wind mediated erosion process of ordinary quartz crystals was proposed to produce activated quartz grains, which sequester methane by forming covalent Si-C bonds. If this process is op-erational on Mars today, which some recent prelimi-nary studies on

  8. Economic Analysis Case Studies of Battery Energy Storage with SAM

    SciTech Connect

    DiOrio, Nicholas; Dobos, Aron; Janzou, Steven

    2015-11-01

    Interest in energy storage has continued to increase as states like California have introduced mandates and subsidies to spur adoption. This energy storage includes customer sited behind-the-meter storage coupled with photovoltaics (PV). This paper presents case study results from California and Tennessee, which were performed to assess the economic benefit of customer-installed systems. Different dispatch strategies, including manual scheduling and automated peak-shaving were explored to determine ideal ways to use the storage system to increase the system value and mitigate demand charges. Incentives, complex electric tariffs, and site specific load and PV data were used to perform detailed analysis. The analysis was performed using the free, publically available System Advisor Model (SAM) tool. We find that installation of photovoltaics with a lithium-ion battery system priced at $300/kWh in Los Angeles under a high demand charge utility rate structure and dispatched using perfect day-ahead forecasting yields a positive net-present value, while all other scenarios cost the customer more than the savings accrued. Different dispatch strategies, including manual scheduling and automated peak-shaving were explored to determine ideal ways to use the storage system to increase the system value and mitigate demand charges. Incentives, complex electric tariffs, and site specific load and PV data were used to perform detailed analysis. The analysis was performed using the free, publically available System Advisor Model (SAM) tool. We find that installation of photovoltaics with a lithium-ion battery system priced at $300/kWh in Los Angeles under a high demand charge utility rate structure and dispatched using perfect day-ahead forecasting yields a positive net-present value, while all other scenarios cost the customer more than the savings accrued.

  9. ReefSAM - Reef Sedimentary Accretion Model: A new 3D coral reef evolution model/simulator

    NASA Astrophysics Data System (ADS)

    Barrett, Samuel; Webster, Jody

    2013-04-01

    Coral reefs show characteristic morphological patterns (e.g. coral dominated margins with detrital carbonate dominated lagoons/back-reef) and temporal development (e.g. Hopley et al. 2007). While the processes which lead to predictable patterns on a range of scales have been discussed qualitatively, a full quantitative understanding of the range of processes and parameters involved requires modelling. Previous attempts to model complex Holocene reef systems (i.e. One Tree Reef, GBR - Barrett and Webster 2012) using a carbonate stratigraphic forward model (Carbonate3D - Warrlich et al. 2002) identified a number of important but unsimulated processes and potential model improvements. ReefSAM has been written from scratch in Matlab using these findings and experiences from using Carbonate3D. It simulates coralgal accretion and carbonate sand production and transport. Specific improvements include: 1. a more complex hydrodynamic model based on wave refraction and incorporating vertical (depth) and lateral (substrate dependent) variations in transport energy and erosion. 2. a complex reef growth model incorporating depth, wave energy/turbidity and substrate composition. 3. Paleo-water depth, paleo-wave energy and bio-zone (combination of paleo-water depth and wave energy) model outputs allowing coralgal habitat changes through time and space to be simulated and compared to observational data. The model is compared to the well studied One Tree Reef - tests similar to those undertaken in Barrett and Webster 2012 with Carbonate3D are presented. Model development coincides with plans for further intensive drilling at One Tree Reef (mid 2013) providing an opportunity to test the model predictively. The model is still in active development. References: Barrett, S.J., Webster, J.M.,2012. Holocene evolution of the Great Barrier Reef: Insights from 3D numerical modelling. Sedimentary Geology 265-266, 56-71. Warrlich, G.M.D., Waltham, D.A., Bosence D.W.J., 2002. Quantifying the

  10. The SAM software system for modeling severe accidents at nuclear power plants equipped with VVER reactors on full-scale and analytic training simulators

    NASA Astrophysics Data System (ADS)

    Osadchaya, D. Yu.; Fuks, R. L.

    2014-04-01

    The architecture of the SAM software package intended for modeling beyond-design-basis accidents at nuclear power plants equipped with VVER reactors evolving into a severe stage with core melting and failure of the reactor pressure vessel is presented. By using the SAM software package it is possible to perform comprehensive modeling of the entire emergency process from the failure initiating event to the stage of severe accident involving meltdown of nuclear fuel, failure of the reactor pressure vessel, and escape of corium onto the concrete basement or into the corium catcher with retention of molten products in it.

  11. Pilot weather advisor

    NASA Technical Reports Server (NTRS)

    Kilgore, W. A.; Seth, S.; Crabill, N. L.; Shipley, S. T.; Graffman, I.; Oneill, J.

    1992-01-01

    The results of the work performed by ViGYAN, Inc., to demonstrate the Pilot Weather Advisor cockpit weather data system using a broadcast satellite communication system are presented. The Pilot Weather Advisor demonstrated that the technical problems involved with transmitting significant amount of weather data to an aircraft in-flight or on-the-ground via satellite are solvable with today's technology. The Pilot Weather Advisor appears to be a viable solution for providing accurate and timely weather information for general aviation aircraft.

  12. Outcome Effectiveness of the Lay Health Advisor Model among Latinos in the United States: An Examination by Role

    ERIC Educational Resources Information Center

    Ayala, Guadalupe X.; Vaz, Lara; Earp, Jo Anne; Elder, John P.; Cherrington, Andrea

    2010-01-01

    The objective of this study was to examine lay health advisor (LHA) programs designed to promote health among US Latinos and the extent to which educator-only versus educator-plus-bridge programs differed in designs and outcomes achieved. Two independent coders reviewed 128 published articles on LHAs yielding information at two levels: (i) study…

  13. SAP Minutes No.2015-03 for FIFRA meeting held 9/15-17/2015. A set of scientific issues being considered by the Environmental Protection Agency regarding development of a spatial aquatic model(SAM)for pesticide risk assessment

    Technology Transfer Automated Retrieval System (TEKTRAN)

    On September 15-17th, 2014, the US Environmental Protection Agency convened a public meeting of the FIFRA Scientific Advisory Panel (SAP) to address scientific issues associated with the agency’s “Development of a Spatial Aquatic Model (SAM) for Pesticide Risk Assessment”. The goal of SAM is to impr...

  14. Online Instructors as Thinking Advisors: A Model for Online Learner Adaptation

    ERIC Educational Resources Information Center

    Benedetti, Christopher

    2015-01-01

    This article examines the characteristics and challenges of online instruction and presents a model for improving learner adaptation in an online classroom. Instruction in an online classroom presents many challenges, including learner individualization. Individual differences in learning styles and preferences are often not considered in the…

  15. Heterotypic Sam-Sam association between Odin-Sam1 and Arap3-Sam: binding affinity and structural insights.

    PubMed

    Mercurio, Flavia A; Marasco, Daniela; Pirone, Luciano; Scognamiglio, Pasqualina L; Pedone, Emilia M; Pellecchia, Maurizio; Leone, Marilisa

    2013-01-01

    Arap3 is a phosphatidylinositol 3 kinase effector protein that plays a role as GTPase activator (GAP) for Arf6 and RhoA. Arap3 contains a sterile alpha motif (Sam) domain that has high sequence homology with the Sam domain of the EphA2-receptor (EphA2-Sam). Both Arap3-Sam and EphA2-Sam are able to associate with the Sam domain of the lipid phosphatase Ship2 (Ship2-Sam). Recently, we reported a novel interaction between the first Sam domain of Odin (Odin-Sam1), a protein belonging to the ANKS (ANKyrin repeat and Sam domain containing) family, and EphA2-Sam. In our latest work, we applied NMR spectroscopy, surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) to characterize the association between Arap3-Sam and Odin-Sam1. We show that these two Sam domains interact with low micromolar affinity. Moreover, by means of molecular docking techniques, supported by NMR data, we demonstrate that Odin-Sam1 and Arap3-Sam might bind with a topology that is common to several Sam-Sam complexes. The revealed structural details form the basis for the design of potential peptide antagonists that could be used as chemical tools to investigate functional aspects related to heterotypic Arap3-Sam associations.

  16. Advisors as Interaction Designers

    ERIC Educational Resources Information Center

    Shockley-Zalabak, Pamela

    2012-01-01

    Pamela Shockley-Zalabak, Chancellor, University of Colorado Colorado Springs, gave the following speech at the 2011 NACADA Annual Conference October 3, 2011, at the Colorado Convention Center in Denver. She provides an overview of changes affecting U.S. higher education, commentary on how those changes affect the role of the academic advisor, and…

  17. The American Advisor Abroad.

    ERIC Educational Resources Information Center

    Bass, Bernard M.

    Mutual understanding of both similarities and differences between Americans and host nation counterparts is a key to effective use of American advisors abroad. When Americans and their hosts know what to expect of each other, the interpersonal competence, consulting skill, and technical knowledge of American consultants can be brought to bear more…

  18. Critics and advisors: Heuristic knowledge and manufacturability

    SciTech Connect

    Rivera, J.J.; Stubblefield, W.A.; Ames, A.L.

    1996-06-01

    In recent years, much of the progress in Computer-Aided Manufacturing has emphasized the use of simulation, finite-element analysis, and other science-based techniques to plan and evaluate manufacturing processes. These approaches are all based on the idea that we can build sufficiently faithful models of complex manufacturing processes such as machining, welding, and casting. Although there has been considerable progress in this area, it continues to suffer from difficulties: the first of these is that the kind of highly accurate models that this approach requires may take many person months to construct, and the second is the large amount of computing resources needed to run these simulations. Two design advisors, Near Net-Shape Advisor and Design for Machinability Advisor, are being developed to explore the role of heuristic, knowledge-based systems for manufacturing processes, both as an alternative to more analytical techniques, and also in support of these techniques. Currently the advisors are both in the prototype stage. All indications lead to the conclusion that the advisors will be successful and lay the groundwork for additional systems such as these in the future.

  19. Synthia Tonn, SAM Engineer

    NASA Video Gallery

    The Sample Analysis at Mars (SAM) is a suite of instruments developed for use on the Mars Science Laboratory, designed to help find out whether or not Mars ever supported life. This video profiles ...

  20. Fast and slow responses of Southern Ocean sea surface temperature to SAM in coupled climate models

    NASA Astrophysics Data System (ADS)

    Krishnamurti, T. N.; Kumar, Vinay; Simon, Anu; Thomas, Aype; Bhardwaj, Amit; Das, Sweta; Senroy, Soma; Roy Bhowmik, S. K.

    2016-06-01

    A major rain storm in Uttarakhand (India) caused heavy rains and major loss of life from floods and land slide during 16-18 June, 2013. The observed daily maximum rainfall rates (3-hourly) during the 16th and 17th June were 220 and 340 mm respectively. This event is addressed via sensitivity studies using a cloud resolving non-hydrostatic model with detailed microphysics. The streaming of moist air from the east-south-east and warmer air from the south-west contributed to the sustained large population and amplitude of buoyancy and the associated CAPE contributed to the longer period of heavy rains. This study addresses the concept of Buoyancy as a means for large vertical accelerations, stronger vertical motions, extreme rain rates and the mechanisms that relate to the time rates of change. A post-processing algorithm provides an analysis of time rate of change for the buoyancy. Moist air streams and warm/moist air intrusions into heavily raining clouds are part of this buoyancy enhancement framework. Improvements in modeling of the extreme rain event came from adaptive observational strategy that showed lack of moisture data sets in these vital regions. We show that a moist boundary layer near the Bay of Bengal leads to moist rivers of moisture where the horizontal convergence confines a large population of buoyancy elements with large magnitudes of buoyancy that streams towards the region of extreme orographic rains. The areas covered in this study include: (i) Use of high resolution cloud modeling (1-km), (ii) Now casting of rains using physical initialization with a Newtonian relaxation, (iii) Use of an adaptive observational strategy, (iii) Sensitivity of the evolution of fields and population of buoyancy elements to boundary layer moisture, (iv) Role of orography and details of buoyancy budget.

  1. Training Public Health Advisors

    PubMed Central

    Meyer, Pamela A.; Brusuelas, Kristin M.; Baden, Daniel J.; Duncan, Heather L.

    2015-01-01

    Federal public health advisors provide guidance and assistance to health departments to improve public health program work. The Centers for Disease Control and Prevention (CDC) prepares them with specialized training in administering public health programs. This article describes the evolving training and is based on internal CDC documents and interviews. The first federal public health advisors worked in health departments to assist with controlling syphilis after World War II. Over time, more CDC prevention programs hired them. To meet emerging needs, 3 major changes occurred: the Public Health Prevention Service, a fellowship program, in 1999; the Public Health Associate Program in 2007; and integration of those programs. Key components of the updated training are competency-based training, field experience, supervision, recruitment and retention, and stakeholder support. The enduring strength of the training has been the experience in a public health agency developing practical skills for program implementation and management. PMID:25564995

  2. A Thermodynamics Model for the Emergence of a Stripe-like Binary SAM on a Nanoparticle Surface.

    PubMed

    Ge, Xinwei; Ke, Pu Chun; Davis, Thomas P; Ding, Feng

    2015-10-01

    It has been under debate if a self-assembled monolayer (SAM) with two immiscible ligands of different chain lengths and/or bulkiness can form a stripe-like pattern on a nanoparticle (NP) surface. The entropic gain upon such pattern formation due to difference in chain lengths and/or bulkiness has been proposed as the driving force in literature. Using atomistic discrete molecular dynamics simulations it is shown that stripe-like pattern could indeed emerge, but only for a subset of binary SAM systems. In addition to entropic contributions, the formation of a striped pattern also strongly depends upon interligand interactions governed by the physicochemical properties of the ligand constituents. Due to the interplay between entropy and enthalpy, a binary SAM system can be categorized into three different types depending on whether and under what condition a striped pattern can emerge. The results help clarify the ongoing debate and our proposed principle can aid in the engineering of novel binary SAMs on a NP surface.

  3. The senescence-accelerated mouse (SAM): a higher oxidative stress and age-dependent degenerative diseases model.

    PubMed

    Chiba, Yoichi; Shimada, Atsuyoshi; Kumagai, Naoko; Yoshikawa, Keisuke; Ishii, Sanae; Furukawa, Ayako; Takei, Shiro; Sakura, Masaaki; Kawamura, Noriko; Hosokawa, Masanori

    2009-04-01

    The SAM strain of mice is actually a group of related inbred strains consisting of a series of SAMP (accelerated senescence-prone) and SAMR (accelerated senescence-resistant) strains. Compared with the SAMR strains, the SAMP strains show a more accelerated senescence process, a shorter lifespan, and an earlier onset and more rapid progress of age-associated pathological phenotypes similar to human geriatric disorders. The higher oxidative stress status observed in SAMP mice is partly caused by mitochondrial dysfunction, and may be a cause of this senescence acceleration and age-dependent alterations in cell structure and function. Based on our recent observations, we discuss a possible mechanism for mitochondrial dysfunction resulting in the excessive production of reactive oxygen species, and a role for the hyperoxidative stress status in neurodegeneration in SAMP mice. These SAM strains can serve as a useful tool to understand the cellular mechanisms of age-dependent degeneration, and to develop clinical interventions. PMID:18688709

  4. Adapting SAM for CDF

    SciTech Connect

    D. Bonham et al.

    2003-10-13

    The CDF and D0 experiments probe the high-energy frontier and as they do so have accumulated hundreds of Terabytes of data on the way to petabytes of data over the next two years. The experiments have made a commitment to use the developing Grid based on the SAM system to handle these data. The D0 SAM has been extended for use in CDF as common patterns of design emerged to meet the similar requirements of these experiments. The process by which the merger was achieved is explained with particular emphasis on lessons learned concerning the database design patterns plus realization of the use cases.

  5. Pilot Weather Advisor System

    NASA Technical Reports Server (NTRS)

    Lindamood, Glenn; Martzaklis, Konstantinos Gus; Hoffler, Keith; Hill, Damon; Mehrotra, Sudhir C.; White, E. Richard; Fisher, Bruce D.; Crabill, Norman L.; Tucholski, Allen D.

    2006-01-01

    The Pilot Weather Advisor (PWA) system is an automated satellite radio-broadcasting system that provides nearly real-time weather data to pilots of aircraft in flight anywhere in the continental United States. The system was designed to enhance safety in two distinct ways: First, the automated receipt of information would relieve the pilot of the time-consuming and distracting task of obtaining weather information via voice communication with ground stations. Second, the presentation of the information would be centered around a map format, thereby making the spatial and temporal relationships in the surrounding weather situation much easier to understand

  6. Communication Interface for SAMS

    NASA Technical Reports Server (NTRS)

    Koffman, M.; Hartley, F.

    1995-01-01

    An interface is described that supplies communications between the flight instruments and the analog input of an existing conventional recording unit for the Shuttle Space Acceleration Measurement System (SAMS), a data acquisition unit. The architecture and current implementation of an STD bus/LonTalk communication interface are described.

  7. Peptide Fragments of Odin-Sam1: Conformational Analysis and Interaction Studies with EphA2-Sam.

    PubMed

    Mercurio, Flavia A; Di Natale, Concetta; Pirone, Luciano; Scognamiglio, Pasqualina L; Marasco, Daniela; Pedone, Emilia M; Saviano, Michele; Leone, Marilisa

    2015-07-27

    Odin is a protein belonging to the ANKS family, and has two tandem Sam domains. The first, Odin-Sam1, binds to the Sam domain of the EphA2 receptor (EphA2-Sam); this interaction could be crucial for the regulation of receptor endocytosis and might have an impact on cancer. Odin-Sam1 associates with EphA2-Sam by adopting a "mid-loop/end-helix" model. In this study three peptide sequences, encompassing the mid-loop interacting portion of Odin-Sam1 and its C-terminal α5 helix, were designed. Their conformational properties were analyzed by CD and NMR. In addition, their abilities to interact with EphA2-Sam were investigated by SPR studies. The peptides adopt a predominantly disordered state in aqueous buffer, but a higher helical content is evident in the presence of the cosolvent trifluoroethanol. Dissociation constants towards EphA2-Sam were in the high micromolar range. The structural findings suggest further routes for the design of potential anti-cancer therapeutics as inhibitors of EphA2-Sam heterotypic interactions.

  8. Peptide Fragments of Odin-Sam1: Conformational Analysis and Interaction Studies with EphA2-Sam.

    PubMed

    Mercurio, Flavia A; Di Natale, Concetta; Pirone, Luciano; Scognamiglio, Pasqualina L; Marasco, Daniela; Pedone, Emilia M; Saviano, Michele; Leone, Marilisa

    2015-07-27

    Odin is a protein belonging to the ANKS family, and has two tandem Sam domains. The first, Odin-Sam1, binds to the Sam domain of the EphA2 receptor (EphA2-Sam); this interaction could be crucial for the regulation of receptor endocytosis and might have an impact on cancer. Odin-Sam1 associates with EphA2-Sam by adopting a "mid-loop/end-helix" model. In this study three peptide sequences, encompassing the mid-loop interacting portion of Odin-Sam1 and its C-terminal α5 helix, were designed. Their conformational properties were analyzed by CD and NMR. In addition, their abilities to interact with EphA2-Sam were investigated by SPR studies. The peptides adopt a predominantly disordered state in aqueous buffer, but a higher helical content is evident in the presence of the cosolvent trifluoroethanol. Dissociation constants towards EphA2-Sam were in the high micromolar range. The structural findings suggest further routes for the design of potential anti-cancer therapeutics as inhibitors of EphA2-Sam heterotypic interactions. PMID:26120079

  9. Outcome effectiveness of the lay health advisor model among Latinos in the United States: an examination by role

    PubMed Central

    Ayala, Guadalupe X.; Vaz, Lara; Earp, Jo Anne; Elder, John P.; Cherrington, Andrea

    2010-01-01

    The objective of this study was to examine lay health advisor (LHA) programs designed to promote health among US Latinos and the extent to which educator-only versus educator-plus-bridge programs differed in designs and outcomes achieved. Two independent coders reviewed 128 published articles on LHAs yielding information at two levels: (i) study design and participant and LHA characteristics from 61 studies that broadly compared educator-only versus educator-plus-bridge programs and (ii) implementation features and outcomes from 17 randomized controlled trials or quasi-experimental studies with outcome data. LHA programs have been widely used with Latinos in certain US regions; our findings indicate that LHAs are effective intervention agents. We identified differences between educator-only and educator-plus-bridge LHA programs, although the small number of educator-plus-bridge programs with outcome data limited comparisons. Major gaps remain in research targeting Latino subgroups other than Mexican immigrants/Mexican Americans. Sufficient research has evaluated LHA programs among Latinos on their ability to achieve health behavior and/or health status changes. In the future, more of a focus on organizational and policy changes is warranted. Questions remain about diversity in LHAs’ characteristics and roles, which influence not only outcomes but also program sustainability and dissemination. PMID:20603384

  10. The Traffic Management Advisor

    NASA Technical Reports Server (NTRS)

    Nedell, William; Erzberger, Heinz; Neuman, Frank

    1990-01-01

    The traffic management advisor (TMA) is comprised of algorithms, a graphical interface, and interactive tools for controlling the flow of air traffic into the terminal area. The primary algorithm incorporated in it is a real-time scheduler which generates efficient landing sequences and landing times for arrivals within about 200 n.m. from touchdown. A unique feature of the TMA is its graphical interface that allows the traffic manager to modify the computer-generated schedules for specific aircraft while allowing the automatic scheduler to continue generating schedules for all other aircraft. The graphical interface also provides convenient methods for monitoring the traffic flow and changing scheduling parameters during real-time operation.

  11. The Advisor Quality Survey: Good College Advisors Are Available, Knowledgeable, and Autonomy Supportive

    ERIC Educational Resources Information Center

    Sheldon, Kennon M.; Garton, Bryan; Orr, Rachael; Smith, Amy

    2015-01-01

    Most US institutions of higher education do not assess advisor quality. We report a scale development effort informed by the developmental prescriptions of self-determination theory (Deci & Ryan, 1985, 2000). The 15-item Missouri Advisor Quality Survey assesses advisor knowledge, advisor availability, and advisor autonomy supportiveness.…

  12. Spot and Runway Departure Advisor

    NASA Technical Reports Server (NTRS)

    Jung, Yoon Chul

    2013-01-01

    The Spot and Runway Departure Advisor (SARDA) is a research prototype of a decision support tool for ATC tower controllers to assist in manging and controlling traffic on the surface of an airport. SARDA employs a scheduler to generate an optimal runway schedule and gate push-back - spot release sequence and schedule that improves efficiency of surface operations. The advisories for ATC tower controllers are displayed on an Electronic Flight Strip (EFS) system. The human-in-the-loop simulation of the SARDA tool was conducted for east operations of Dallas-Ft. Worth International Airport (DFW) to evaluate performance of the SARDA tool and human factors, such as situational awareness and workload. The results indicates noticeable taxi delay reduction and fuel savings by using the SARDA tool. Reduction in controller workload were also observed throughout the scenario runs. The future plan includes modeling and simulation of the ramp operations of the Charlotte International Airport, and develop a decision support tool for the ramp controllers.

  13. A Business Advisor's Guide to Counseling Theories.

    ERIC Educational Resources Information Center

    Boyd, John A.

    For the small business advisor, consulting and counseling are part of the same continuum. Advisors' roles can be aligned hierarchically: (1) advisors may need simply to provide business information or expertise; (2) they may need to facilitate the business process, helping clients identify problems and discover their own solutions; (3) for clients…

  14. SAM II Data and Information

    Atmospheric Science Data Center

    2016-07-06

    ... Data obtained from the Stratospheric Aerosol Measurement (SAM) II instrument, which flew on board the Nimbus-7 ... Spatial Resolution:  The altitude profiles of aerosol extinction have a 1 km vertical resolution. Temporal ...

  15. Faces of Marshall: Sam Ortega

    NASA Video Gallery

    Several Marshall employees were interviewed as part of Marshall's 50th Anniversary activities. Engineer Sam Ortega tells his story of how he came to work as an engineer at Marshall and how sewing a...

  16. The entomologist as a science partner and curriculum advisor: The Earth School model for grades 6--8

    NASA Astrophysics Data System (ADS)

    Marshall, Bethany Johnston

    The Earth School model for creation of partnerships between university scientists and public schools began with a traditional research project involving the study of macroinvertebrate recolonization of agriculturally based restored wetlands. From fieldwork designed to address hypotheses of community composition over time, protocols and equipment evolved for application in middle-school classrooms. In addition to classroom teachers guiding their students in replicating active scientific research, the inclusion of a science partner was key to the success of this model. To ensure that the classroom teachers were themselves comfortable as researchers, monthly staff development workshops were conducted as a component of the Earth School model. The use of entomology as a unifying theme for educational scientific investigation lets the student explore virtually every other system in the biosphere. Because of the unparalleled survivability and adaptability of insects, we can find examples from all biomes, all time references and all disciplines. Over the course of long-term continuous exploration, learners become familiar with relationships and patterns evident in natural situations. These same patterns of birth, growth and decay are much more vividly demonstrated in the field than in textbooks. Similarly, concrete examples of feeding relationships between organisms are plentiful in nearly any outdoor situation. The following model incorporates current research from multiple scientific disciplines but focuses on the many and varied research activities offered by the entomological community. Teachers and students in a primarily urban setting made extensive use of the materials developed through the course of this model's development. Their feedback as the materials were integrated into an established curriculum allowed for the fine-tuning of activity development. A conversion template has evolved that gives teachers, curriculum directors, parents and other educators a

  17. Testing new approaches to carbonate system simulation at the reef scale: the ReefSam model first results, application to a question in reef morphology and future challenges.

    NASA Astrophysics Data System (ADS)

    Barrett, Samuel; Webster, Jody

    2016-04-01

    Numerical simulation of the stratigraphy and sedimentology of carbonate systems (carbonate forward stratigraphic modelling - CFSM) provides significant insight into the understanding of both the physical nature of these systems and the processes which control their development. It also provides the opportunity to quantitatively test conceptual models concerning stratigraphy, sedimentology or geomorphology, and allows us to extend our knowledge either spatially (e.g. between bore holes) or temporally (forwards or backwards in time). The later is especially important in determining the likely future development of carbonate systems, particularly regarding the effects of climate change. This application, by its nature, requires successful simulation of carbonate systems on short time scales and at high spatial resolutions. Previous modelling attempts have typically focused on the scales of kilometers and kilo-years or greater (the scale of entire carbonate platforms), rather than at the scale of centuries or decades, and tens to hundreds of meters (the scale of individual reefs). Previous work has identified limitations in common approaches to simulating important reef processes. We present a new CFSM, Reef Sedimentary Accretion Model (ReefSAM), which is designed to test new approaches to simulating reef-scale processes, with the aim of being able to better simulate the past and future development of coral reefs. Four major features have been tested: 1. A simulation of wave based hydrodynamic energy with multiple simultaneous directions and intensities including wave refraction, interaction, and lateral sheltering. 2. Sediment transport simulated as sediment being moved from cell to cell in an iterative fashion until complete deposition. 3. A coral growth model including consideration of local wave energy and composition of the basement substrate (as well as depth). 4. A highly quantitative model testing approach where dozens of output parameters describing the reef

  18. TEACHER-ADVISORS: Where There's a Skill There's A Way.

    ERIC Educational Resources Information Center

    Tamminen, Armas; And Others

    This report discusses a program to present the Teacher Advisement Training Model. This model for training teacher-advisors is based on the assumption that tentative commitment to making school a more rewarding experience for all is the first step in starting an effective program. The approach is to help teachers learn specific skills and methods…

  19. Threat expert system technology advisor

    NASA Technical Reports Server (NTRS)

    Kurrasch, E. R.; Tripp, L. R.

    1987-01-01

    A prototype expert system was developed to determine the feasibility of using expert system technology to enhance the performance and survivability of helicopter pilots in a combat threat environment while flying NOE (Nap of the Earth) missions. The basis for the concept is the potential of using an Expert System Advisor to reduce the extreme overloading of the pilot who flies NOE mission below treetop level at approximately 40 knots while performing several other functions. The ultimate goal is to develop a Threat Expert System Advisor which provides threat information and advice that are better than even a highly experienced copilot. The results clearly show that the NOE pilot needs all the help in decision aiding and threat situation awareness that he can get. It clearly shows that heuristics are important and that an expert system for combat NOE helicopter missions can be of great help to the pilot in complex threat situations and in making decisions.

  20. Omega-3 polyunsaturated fatty acids ameliorate the severity of ileitis in the senescence accelerated mice (SAM)P1/Yit mice model

    PubMed Central

    Matsunaga, H; Hokari, R; Kurihara, C; Okada, Y; Takebayashi, K; Okudaira, K; Watanabe, C; Komoto, S; Nakamura, M; Tsuzuki, Y; Kawaguchi, A; Nagao, S; Miura, S

    2009-01-01

    Clinical studies using omega-3 polyunsaturated fatty acids (ω3-PUFA) to Crohn's disease (CD) are conflicting. Beneficial effects of dietary ω3-PUFA intake in various experimental inflammatory bowel disease (IBD) models have been reported. However, animal models of large intestinal inflammation have been used in all previous studies, and the effect of ω3 fat in an animal model of small intestinal inflammation has not been reported. We hypothesized that the effects of ω3 fat are different between large and small intestine. The aim of this study was to determine whether the direct effect of ω3 fat is beneficial for small intestinal inflammation. Senescence accelerated mice (SAM)P1/Yit mice showed remarkable inflammation of the terminal ileum spontaneously. The numbers of F4/80-positive monocyte–macrophage cells as well as β7-integrin-positive lymphocytes in the intestinal mucosa were increased significantly compared with those in the control mice (AKR-J mice). The area of mucosal addressin cell adhesion molecule-1 (MAdCAM-1)-positive vessels was also increased. The degree of expression levels of monocyte chemoattractant protein-1 (MCP-1), interleukin (IL)-6 and interferon (IFN)-γ mRNA were increased significantly compared with those in the control mice. The feeding of two different kinds of ω3 fat (fish-oil-rich and perilla-oil-rich diets) for 16 weeks to SAMP1/Yit mice ameliorated inflammation of the terminal ileum significantly. In both the ω3-fat-rich diet groups, enhanced infiltration of F4/80-positive monocytes/macrophages in intestinal mucosa of SAMP1/Yit mice cells and the increased levels of MCP-1, IL-6 and IFN-γ mRNA expression were ameliorated significantly compared with those in the control diet group. The results suggest that ω3 fat is beneficial for small intestinal inflammation by inhibition of monocyte recruitment to inflamed intestinal mucosa. PMID:19793338

  1. On LAM's and SAM's for Halley's rotation

    NASA Technical Reports Server (NTRS)

    Peale, Stanton J.

    1992-01-01

    Non principal axis rotation for comet Halley is inferred from dual periodicities evident in the observations. The modes where the spin axis precesses around the axis of minimum moment of inertia (long axis mode or LAM) and where it precesses around the axis of maximum moment of inertia (short axis mode or SAM) are described from an inertial point of view. The currently favored LAM model for Halley's rotation state satisfies observational and dynamical constraints that apparently no SAM can satisfy. But it cannot reproduce the observed post perihelion brightening through seasonal illumination of localized sources on the nucleus, whereas a SAM can easily produce post or pre perihelion brightening by this mechanism. However, the likelihood of a LAM rotation for elongated nuclei of periodic comets such as Halley together with Halley's extreme post perihelion behavior far from the Sun suggest that Halley's post perihelion brightening may be due to effects other than seasonal illumination of localized sources, and therefore such brightening may not constrain its rotation state.

  2. The Role of Doctoral Advisors: A Look at Advising from the Advisor's Perspective

    ERIC Educational Resources Information Center

    Barnes, Benita J.; Austin, Ann E.

    2009-01-01

    The doctoral advisor is said to be one of the most important persons--if not "the" single most critical person--with whom doctoral students will develop a relationship during their doctoral degree programs (Baird 1995). However, we have limited knowledge regarding how doctoral advisors see their roles and responsibilities as advisors. Therefore,…

  3. Flight deck engine advisor

    NASA Technical Reports Server (NTRS)

    Shontz, W. D.; Records, R. M.; Antonelli, D. R.

    1992-01-01

    The focus of this project is on alerting pilots to impending events in such a way as to provide the additional time required for the crew to make critical decisions concerning non-normal operations. The project addresses pilots' need for support in diagnosis and trend monitoring of faults as they affect decisions that must be made within the context of the current flight. Monitoring and diagnostic modules developed under the NASA Faultfinder program were restructured and enhanced using input data from an engine model and real engine fault data. Fault scenarios were prepared to support knowledge base development activities on the MONITAUR and DRAPhyS modules of Faultfinder. An analysis of the information requirements for fault management was included in each scenario. A conceptual framework was developed for systematic evaluation of the impact of context variables on pilot action alternatives as a function of event/fault combinations.

  4. Solution structure of the first Sam domain of Odin and binding studies with the EphA2 receptor.

    PubMed

    Mercurio, Flavia Anna; Marasco, Daniela; Pirone, Luciano; Pedone, Emilia Maria; Pellecchia, Maurizio; Leone, Marilisa

    2012-03-13

    The EphA2 receptor plays key roles in many physiological and pathological events, including cancer. The process of receptor endocytosis and the consequent degradation have attracted attention as possible means of overcoming the negative outcomes of EphA2 in cancer cells and decreasing tumor malignancy. A recent study indicates that Sam (sterile alpha motif) domains of Odin, a member of the ANKS (ankyrin repeat and sterile alpha motif domain-containing) family of proteins, are important for the regulation of EphA2 endocytosis. Odin contains two tandem Sam domains (Odin-Sam1 and -Sam2). Herein, we report on the nuclear magnetic resonance (NMR) solution structure of Odin-Sam1; through a variety of assays (employing NMR, surface plasmon resonance, and isothermal titration calorimetry techniques), we clearly demonstrate that Odin-Sam1 binds to the Sam domain of EphA2 in the low micromolar range. NMR chemical shift perturbation experiments and molecular modeling studies point out that the two Sam domains interact with a head-to-tail topology characteristic of several Sam-Sam complexes. This binding mode is similar to that we have previously proposed for the association between the Sam domains of the lipid phosphatase Ship2 and EphA2. This work further validates structural elements relevant for the heterotypic Sam-Sam interactions of EphA2 and provides novel insights for the design of potential therapeutic compounds that can modulate receptor endocytosis.

  5. Simulation Test Of Descent Advisor

    NASA Technical Reports Server (NTRS)

    Davis, Thomas J.; Green, Steven M.

    1991-01-01

    Report describes piloted-simulation test of Descent Advisor (DA), subsystem of larger automation system being developed to assist human air-traffic controllers and pilots. Focuses on results of piloted simulation, in which airline crews executed controller-issued descent advisories along standard curved-path arrival routes. Crews able to achieve arrival-time precision of plus or minus 20 seconds at metering fix. Analysis of errors generated in turns resulted in further enhancements of algorithm to increase accuracies of its predicted trajectories. Evaluations by pilots indicate general support for DA concept and provide specific recommendations for improvement.

  6. SAM Overview: The Habitability of Mars

    NASA Video Gallery

    Featuring an interview with Paul Mahaffy, SAM's Principal Investigator, this video gives a general overview of SAM's mission aboard the Mars Science Laboratory, otherwise known as the Curiosity rover.

  7. SAM II Data and Information (ASCII)

    Atmospheric Science Data Center

    2016-09-01

    SAM II (ASCII) Data and Information Data obtained from the Stratospheric Aerosol Measurement (SAM) II instrument, ... Guide Documents:  Project Guide Data Set Guide Readme Files:  Data Set (Text file) ...

  8. The Vesicle Protein SAM-4 Regulates the Processivity of Synaptic Vesicle Transport

    PubMed Central

    Zheng, Qun; Ahlawat, Shikha; Schaefer, Anneliese; Mahoney, Tim; Koushika, Sandhya P.; Nonet, Michael L.

    2014-01-01

    Axonal transport of synaptic vesicles (SVs) is a KIF1A/UNC-104 mediated process critical for synapse development and maintenance yet little is known of how SV transport is regulated. Using C. elegans as an in vivo model, we identified SAM-4 as a novel conserved vesicular component regulating SV transport. Processivity, but not velocity, of SV transport was reduced in sam-4 mutants. sam-4 displayed strong genetic interactions with mutations in the cargo binding but not the motor domain of unc-104. Gain-of-function mutations in the unc-104 motor domain, identified in this study, suppress the sam-4 defects by increasing processivity of the SV transport. Genetic analyses suggest that SAM-4, SYD-2/liprin-α and the KIF1A/UNC-104 motor function in the same pathway to regulate SV transport. Our data support a model in which the SV protein SAM-4 regulates the processivity of SV transport. PMID:25329901

  9. SPILC: An expert student advisor

    NASA Technical Reports Server (NTRS)

    Read, D. R.

    1990-01-01

    The Lamar University Computer Science Department serves about 350 undergraduate C.S. majors, and 70 graduate majors. B.S. degrees are offered in Computer Science and Computer and Information Science, and an M.S. degree is offered in Computer Science. In addition, the Computer Science Department plays a strong service role, offering approximately sixteen service course sections per long semester. The department has eight regular full-time faculty members, including the Department Chairman and the Undergraduate Advisor, and from three to seven part-time faculty members. Due to the small number of regular faculty members and the resulting very heavy teaching loads, undergraduate advising has become a difficult problem for the department. There is a one week early registration period and a three-day regular registration period once each semester. The Undergraduate Advisor's regular teaching load of two classes, 6 - 8 semester hours, per semester, together with the large number of majors and small number of regular faculty, cause long queues and short tempers during these advising periods. The situation is aggravated by the fact that entering freshmen are rarely accompanied by adequate documentation containing the facts necessary for proper counselling. There has been no good method of obtaining necessary facts and documenting both the information provided by the student and the resulting advice offered by the counsellors.

  10. Faculty Advisors' Experiences with International Graduate Students

    ERIC Educational Resources Information Center

    Nguyen, Huynh Mai

    2013-01-01

    The current study explored the experiences of faculty advisors working with international graduate students (IGS). Data was collected through semi-structured interviews and a demographic survey and was analyzed using open, axial, and selective coding strategies. Three central categories emerged from the analysis: (a) advisors' perceptions of IGS,…

  11. Student and Advisor Attitudes toward Academic Advisement.

    ERIC Educational Resources Information Center

    Wood, Jane H.; Wood, Peter H.

    Three separate but similar questionnaires were mailed to faculty advisors, to secondary education majors, and to nonsecondary education majors in the College of Education and Allied Professions at Bowling Green State University. The majority of each group identified assistance with courses and scheduling at the advisors' most important function.…

  12. Feature-Based Measuring Advisor, Version 2.1

    2002-07-22

    The purpose of Feature-Based Measuring Advisor (FBMeas Advisor) is a graphical user interface enabling users to more effectively interact with a solid model for measuring applications. Specifically, the FBMeas Advisor provides a user interface to the functions and capabilities of the FBMeas component technology. The FBMeas Advisor allows end users to visualize, organize, and document their measuring process plans and to facilitate the generation of a coordinate measuring machine part programs before a workpiece ismore » fabricated. The FBMeas Advisor permits the user to interact with an FBTol-enabled solid model to interactively and/or automatically create a set of measuring features (e.g., cylinders, planes, slots) that comply with national and international interface standards (i.e., DMIS (Dimensional Measuring Interface Standard, ISO 22063)). Measuring plans include defining measuring features interactively or automatically. Associating measuring features with appropriate tolerances and datum features via FBTol. Determining what measuring tasks (e.g., measure this feature to validate a size tolerance, measure this feature to establish a primary datum for a specific datum reference frame) are necessary to measure the feature. Assigning a measuring method to perform the task. For coordinate metrology measuring methods, measure points are determined interactively or automatically. Sensors and sensor configurations are defined, represented, and assigned to measuring methods. Measuring machines are defined, represented, and assigned to measure operations. Upon completion of a coordinate metrology measuring plan, FBMeas allows DMIS code segments to be generated, which can be used for DMIS-based CMM part programs.« less

  13. The nutrition advisor expert system

    NASA Technical Reports Server (NTRS)

    Huse, Scott M.; Shyne, Scott S.

    1991-01-01

    The Nutrition Advisor Expert System (NAES) is an expert system written in the C Language Integrated Production System (CLIPS). NAES provides expert knowledge and guidance into the complex world of nutrition management by capturing the knowledge of an expert and placing it at the user's fingertips. Specifically, NAES enables the user to: (1) obtain precise nutrition information for food items; (2) perform nutritional analysis of meal(s), flagging deficiencies based upon the U.S. Recommended Daily Allowances; (3) predict possible ailments based upon observed nutritional deficiency trends; (4) obtain a top ten listing of food items for a given nutrient; and (5) conveniently upgrade the data base. An explanation facility for the ailment prediction feature is also provided to document the reasoning process.

  14. SAMS Acceleration Measurements on MIR

    NASA Technical Reports Server (NTRS)

    Moskowitz, Milton E.; Hrovat, Kenneth; Finkelstein, Robert; Reckart, Timothy

    1997-01-01

    During NASA Increment 3 (September 1996 to January 1997), about 5 gigabytes of acceleration data were collected by the Space Acceleration Measurement System (SAMS) onboard the Russian Space Station, Mir. The data were recorded on 11 optical disks and were returned to Earth on STS-81. During this time, SAMS data were collected in the Priroda module to support the following experiments: the Mir Structural Dynamics Experiment (MiSDE) and Binary Colloidal Alloy Tests (BCAT). This report points out some of the salient features of the microgravity environment to which these experiments were exposed. Also documented are mission events of interest such as the docked phase of STS-81 operations, a Progress engine burn, attitude control thruster operation, and crew exercise. Also included are a description of the Mir module orientations, and the panel notations within the modules. This report presents an overview of the SAMS acceleration measurements recorded by 10 Hz and 100 Hz sensor heads. Variations in the acceleration environment caused by unique activities such as crew exercise and life-support fans are presented. The analyses included herein complement those presented in previous mission summary reports published by the Principal Investigator Microgravity Services (PIMS) group.

  15. Sam.

    ERIC Educational Resources Information Center

    Wilson, James C.

    1988-01-01

    A father writes about his six-year-old son born with hydrocephalus. He describes such day-to-day experiences as going to a baseball game and the grocery store, reactions of friends and strangers to his son's social interactions, and a special day at preschool. The boy's medical treatment, including surgeries, are also described. (VW)

  16. Descent Advisor Preliminary Field Test

    NASA Technical Reports Server (NTRS)

    Green, Steven M.; Vivona, Robert A.; Sanford, Beverly

    1995-01-01

    A field test of the Descent Advisor (DA) automation tool was conducted at the Denver Air Route Traffic Control Center in September 1994. DA is being developed to assist Center controllers in the efficient management and control of arrival traffic. DA generates advisories, based on trajectory predictions, to achieve accurate meter-fix arrival times in a fuel efficient manner while assisting the controller with the prediction and resolution of potential conflicts. The test objectives were: (1) to evaluate the accuracy of DA trajectory predictions for conventional and flight-management system equipped jet transports, (2) to identify significant sources of trajectory prediction error, and (3) to investigate procedural and training issues (both air and ground) associated with DA operations. Various commercial aircraft (97 flights total) and a Boeing 737-100 research aircraft participated in the test. Preliminary results from the primary test set of 24 commercial flights indicate a mean DA arrival time prediction error of 2.4 seconds late with a standard deviation of 13.1 seconds. This paper describes the field test and presents preliminary results for the commercial flights.

  17. Descent advisor preliminary field test

    NASA Technical Reports Server (NTRS)

    Green, Steven M.; Vivona, Robert A.; Sanford, Beverly

    1995-01-01

    A field test of the Descent Advisor (DA) automation tool was conducted at the Denver Air Route Traffic Control Center in September 1994. DA is being developed to assist Center controllers in the efficient management and control of arrival traffic. DA generates advisories, based on trajectory predictions, to achieve accurate meter-fix arrival times in a fuel efficient manner while assisting the controller with the prediction and resolution of potential conflicts. The test objectives were to evaluate the accuracy of DA trajectory predictions for conventional- and flight-management-system-equipped jet transports, to identify significant sources of trajectory prediction error, and to investigate procedural and training issues (both air and ground) associated with DA operations. Various commercial aircraft (97 flights total) and a Boeing 737-100 research aircraft participated in the test. Preliminary results from the primary test set of 24 commercial flights indicate a mean DA arrival time prediction error of 2.4 sec late with a standard deviation of 13.1 sec. This paper describes the field test and presents preliminary results for the commercial flights.

  18. Design of a knowledge-based welding advisor

    SciTech Connect

    Kleban, S.D.

    1996-06-01

    Expert system implementation can take numerous forms ranging form traditional declarative rule-based systems with if-then syntax to imperative programming languages that capture expertise in procedural code. The artificial intelligence community generally thinks of expert systems as rules or rule-bases and an inference engine to process the knowledge. The welding advisor developed at Sandia National Laboratories and described in this paper deviates from this by codifying expertise using object representation and methods. Objects allow computer scientists to model the world as humans perceive it giving us a very natural way to encode expert knowledge. The design of the welding advisor, which generates and evaluates solutions, will be compared and contrasted to a traditional rule- based system.

  19. SAM-2 ground-truth plan: Correlative measurements for the Stratospheric Aerosol Measurement-2 (SAM 2) sensor on the Nimbus G satellite

    NASA Technical Reports Server (NTRS)

    Russell, P. B.; Mccormick, M. P.; Mcmaster, L. R.; Pepin, T. J.; Chu, W. P.; Swissler, T. J.

    1978-01-01

    The SAM-2 will fly aboard the Nimbus-G satellite for launch in the fall of 1978 and measure stratospheric vertical profiles of aerosol extinction in high latitude bands. The plan gives details of the location and times for the simultaneous satellite/correlative measurements for the nominal launch time, the rationale and choice of the correlative sensors, their characteristics and expected accuracies, and the conversion of their data to extinction profiles. The SAM-2 expected instrument performance and data inversion results are presented. Various atmospheric models representative of polar stratospheric aerosols are used in the SAM-2 and correlative sensor analyses.

  20. Surface Hold Advisor Using Critical Sections

    NASA Technical Reports Server (NTRS)

    Law, Caleb Hoi Kei (Inventor); Hsiao, Thomas Kun-Lung (Inventor); Mittler, Nathan C. (Inventor); Couluris, George J. (Inventor)

    2013-01-01

    The Surface Hold Advisor Using Critical Sections is a system and method for providing hold advisories to surface controllers to prevent gridlock and resolve crossing and merging conflicts among vehicles traversing a vertex-edge graph representing a surface traffic network on an airport surface. The Advisor performs pair-wise comparisons of current position and projected path of each vehicle with other surface vehicles to detect conflicts, determine critical sections, and provide hold advisories to traffic controllers recommending vehicles stop at entry points to protected zones around identified critical sections. A critical section defines a segment of the vertex-edge graph where vehicles are in crossing or merging or opposite direction gridlock contention. The Advisor detects critical sections without reference to scheduled, projected or required times along assigned vehicle paths, and generates hold advisories to prevent conflicts without requiring network path direction-of-movement rules and without requiring rerouting, rescheduling or other network optimization solutions.

  1. Characterization of the SAM domain of the PKD-related protein ANKS6 and its interaction with ANKS3

    PubMed Central

    2014-01-01

    Background Autosomal dominant polycystic kidney disease (ADPKD) is the most common genetic disorder leading to end-stage renal failure in humans. In the PKD/Mhm(cy/+) rat model of ADPKD, the point mutation R823W in the sterile alpha motif (SAM) domain of the protein ANKS6 is responsible for disease. SAM domains are known protein-protein interaction domains, capable of binding each other to form polymers and heterodimers. Despite its physiological importance, little is known about the function of ANKS6 and how the R823W point mutation leads to PKD. Recent work has revealed that ANKS6 interacts with a related protein called ANKS3. Both ANKS6 and ANKS3 have a similar domain structure, with ankyrin repeats at the N-terminus and a SAM domain at the C-terminus. Results The SAM domain of ANKS3 is identified as a direct binding partner of the ANKS6 SAM domain. We find that ANKS3-SAM polymerizes and ANKS6-SAM can bind to one end of the polymer. We present crystal structures of both the ANKS3-SAM polymer and the ANKS3-SAM/ANKS6-SAM complex, revealing the molecular details of their association. We also learn how the R823W mutation disrupts ANKS6 function by dramatically destabilizing the SAM domain such that the interaction with ANKS3-SAM is lost. Conclusions ANKS3 is a direct interacting partner of ANKS6. By structurally and biochemically characterizing the interaction between the ANKS3 and ANKS6 SAM domains, our work provides a basis for future investigation of how the interaction between these proteins mediates kidney function. PMID:24998259

  2. Patterning NHS-terminated SAMs on germanium.

    PubMed

    Morris, Carleen J; Shestopalov, Alexander A; Gold, Brian H; Clark, Robert L; Toone, Eric J

    2011-05-17

    Here we report a simple, robust approach to patterning functional SAMs on germanium. The protocol relies on catalytic soft-lithographic pattern transfer from an elastomeric stamp bearing pendant immobilized sulfonic acid moieties to an NHS-functionalized bilayer molecular system comprising a primary ordered alkyl monolayer and a reactive ester secondary overlayer. The catalytic polyurethane-acrylate stamp was used to form micrometer-scale features of chemically distinct SAMs on germanium. The methodology represents the first example of patterned SAMs on germanium, a semiconductor material.

  3. 31 CFR 10.33 - Best practices for tax advisors.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 31 Money and Finance: Treasury 1 2012-07-01 2012-07-01 false Best practices for tax advisors. 10... Service § 10.33 Best practices for tax advisors. (a) Best practices. Tax advisors should provide clients with the highest quality representation concerning Federal tax issues by adhering to best practices...

  4. 31 CFR 10.33 - Best practices for tax advisors.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 31 Money and Finance: Treasury 1 2014-07-01 2014-07-01 false Best practices for tax advisors. 10... Service § 10.33 Best practices for tax advisors. (a) Best practices. Tax advisors should provide clients with the highest quality representation concerning Federal tax issues by adhering to best practices...

  5. 31 CFR 10.33 - Best practices for tax advisors.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 31 Money and Finance: Treasury 1 2011-07-01 2011-07-01 false Best practices for tax advisors. 10... Service § 10.33 Best practices for tax advisors. (a) Best practices. Tax advisors should provide clients with the highest quality representation concerning Federal tax issues by adhering to best practices...

  6. 31 CFR 10.33 - Best practices for tax advisors.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 31 Money and Finance: Treasury 1 2013-07-01 2013-07-01 false Best practices for tax advisors. 10... Service § 10.33 Best practices for tax advisors. (a) Best practices. Tax advisors should provide clients with the highest quality representation concerning Federal tax issues by adhering to best practices...

  7. 31 CFR 10.33 - Best practices for tax advisors.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Best practices for tax advisors. 10... Service § 10.33 Best practices for tax advisors. (a) Best practices. Tax advisors should provide clients with the highest quality representation concerning Federal tax issues by adhering to best practices...

  8. How I Chose My Thesis Advisor

    NASA Astrophysics Data System (ADS)

    Karimabadi, Homa

    2011-01-01

    Professor K. Papadopoulos, or Dennis, who we have all come to him as, has had a profound influence over my career as a scientist as well as my private life. Here I provide a brief account of the events that led me to Dennis as my PhD thesis advisor at University of Maryland and what that has meant to me.

  9. 78 FR 67467 - Registration of Municipal Advisors

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-12

    ... Exchange Act Release No. 62824 (September 1, 2010), 75 FR 54465 (September 8, 2010) (``Temporary... (December 20, 2010), 76 FR 824 (January 6, 2011) (``Proposal''). \\7\\ See Rule 15Ba2-6T and Securities... advisory activities. \\31\\ See Proposal, 76 FR 825. \\32\\ See id. Municipal advisors, commonly referred to...

  10. NES Live Video Chat: Engineer Sam Ortega

    NASA Video Gallery

    The NES project invited all K-12 students to participate in a one-hour-long NASA video webchat on April 19, 2011 with NASA engineer Sam Ortega. Ortega answered questions about building and testing ...

  11. Stay lean without dieting: Lose Sam68.

    PubMed

    Huot, Marc-Étienne; Richard, Stéphane

    2012-10-01

    Alternative splicing is well known to be tissue-specific. Although several genes have been shown to undergo alternative splicing in adipocytes, little is known about the mechanism that regulates alternative splicing during adipogenesis. We recently reported that Sam68(-/-) mice exhibit a lean phenotype and are protected against diet-induced obesity. Our genome-wide exon array analysis in white adipose tissue (WAT) from wild-type and Sam68(-/-) mice revealed that Sam68 deficiency leads to an abnormal splicing of the mTOR gene. This has been shown to reduce the overall mTOR protein content and activity during in vitro adipose differentiation. In Sam68(-/-) mice, this situation leads to an increased energy expenditure, decreased adipogenesis and WAT formation. PMID:23700540

  12. STS-134 Crew Talks With Sam Ting

    NASA Video Gallery

    The STS-134 crew talks with Sam Ting, principal investigator for the Alpha Magnetic Spectrometer, following the installation of the particle physics detector on the International Space Station duri...

  13. Polycystic kidney disease in Han:SPRD Cy rats is associated with elevated expression and mislocalization of SamCystin

    PubMed Central

    Nagao, Shizuko; Morita, Miwa; Kugita, Masanori; Yoshihara, Daisuke; Yamaguchi, Tamio; Kurahashi, Hiroki; Calvet, James P.

    2010-01-01

    Polycystic kidney disease (PKD) in Han:SPRD Cy rats is caused by a missense mutation in Anks6 (also called Pkdr1), leading to an R823W substitution in SamCystin, a protein that contains ankyrin repeats and a sterile alpha motif (SAM). The cellular function of SamCystin and the role of the Cy (R823W) mutation in cyst formation are unknown. In normal SPRD rats, SamCystin was found to be expressed in proximal tubules and glomeruli; protein expression was highest at 7 days of age and declined by ∼50–60% at 45–84 days of age. In Cy/+ and Cy/Cy kidneys, expression of SamCystin was lower than in +/+ kidneys at 3 and 7 days but became elevated at 21 days. Immunohistochemical analysis revealed that SamCystin was distributed on the brush border of proximal tubules in normal rat kidneys. In Cy/+ kidneys, there were robust SamCystin staining in cyst-lining epithelial cells and loss of apical localization, and increased number of PCNA-positive cells in cyst-lining epithelia. Verapamil, an L-type Ca2+ channel blocker, accelerated PKD progression in this model and caused a further increase in the expression and abnormal distribution of SamCystin. We conclude that aberrant expression and mislocalization of R823W SamCystin lead to increased cell proliferation and renal cyst formation. PMID:20719982

  14. Solar Effective Envelope Design Advisor (SEEDA)

    NASA Astrophysics Data System (ADS)

    Mahaek, Ekkachai

    The lack of effort by mainstream architects in integrating energy-efficient strategies in architectural designing is due to the complexity in a building's energy conscious concepts and theories, the difficulties to visualize and quantify energy consumption, and the late implementing of energy consumption analysis in the conventional design process. This task would be accomplishing by a building system's engineer where results might be determined only after the basic architectural design has been completed. An effective simple tool and method should then be available to assist architects in building's energy-efficient designing at the beginning of the design. The building's energy consumption is directly and mainly influenced by the relationship of the sun, site, and its building configuration. The solar radiations will first impact on the building's envelope, which will have a direct effect on the amount of energy a building will consume. If an architect can define or map the intensity of solar energy on the site's buildable volume, and use this information to determine the levels of solar insolation, a more energy efficient building form can be proposed. This research hypothesis has shared the fundamental techniques of the Solar Envelope projection by Professor Ralph Knowles [Knowles, 1981] of the University of Southern California. However a different approach is taken by including the influence of regional restrictions and the surrounding buildings' shadows when projecting of solar volumes and solar envelope. The research methodology will discuss the development of a computer-based approach to develop a three-dimensional architectural form based on an insolation map related to the design site. The prototype computer program is referred as the Solar Effective Envelope Design Advisor (SEEDA). The solar insolation volume of the site is determined by integrating three types of computer-generated models include the Buildable Volume model based on design constraints

  15. Modelo Pedagogico de Educacion Primaria para Adultos: Manual para el Asesor de Estudiantes Libres. Primera Parte (A Pedagogical Model for Adult Primary Education: Manual for the Student Advisor. Part One).

    ERIC Educational Resources Information Center

    Instituto Nacional para la Educacion de los Adultos, Mexico City (Mexico).

    This guide, part of a Mexican series of instructional materials, is intended for advisors of students participating in an adult education program offered through public and private organizations in communities in Mexico. The first part of the program comprises Spanish and math; the second, education for family life, education for community life,…

  16. A Model Mentor/Advisor Program Supporting Secondary School Youth with Emotional and Behavioral Challenges and Their Families within Rural Vermont (October 1, 1996-September 30, 2000). Final Report.

    ERIC Educational Resources Information Center

    Welkowitz, Julie; Fox, Wayne L.

    This final report discusses the activities and outcomes of the Mentor/Advisor Project, a program designed to promote positive self-concept and school success for high school students at risk of or experiencing emotional and behavioral disabilities in rural Vermont. The Project involved small groups of heterogeneous students (i.e., those with and…

  17. Surface adhesion and confinement variation of Staphylococcus aurius on SAM surfaces

    NASA Astrophysics Data System (ADS)

    Amroski, Alicia; Olsen, Morgan; Calabrese, Joseph; Senevirathne, Reshani; Senevirathne, Indrajith

    2012-02-01

    Controlled surface adhesion of non - pathogenic gram positive strain, Staphylococcus aureus is interesting as a model system due to possible development of respective biosensors for prevention and detection of the pathogenic strain methicillin resistant Staphylococcus aureus (MRSA) and further as a study for bio-machine interfacing. Self Assembled Monolayers (SAM) with engineered surfaces of linear thiols on Au(111) were used as the substrate. Sub cultured S. aureus were used for the analysis. The SAM layered surfaces were dipped in 2 -- 4 Log/ml S. aureus solution. Subsequent surface adhesion at different bacterial dilutions on surfaces will be discussed, and correlated with quantitative and qualitative adhesion properties of bacteria on the engineered SAM surfaces. The bacteria adhered SAM surfaces were investigated using intermittent contact, noncontact, lateral force and contact modes of Atomic Force Microscopy (AFM).

  18. The ALICE Glance Shift Accounting Management System (SAMS)

    NASA Astrophysics Data System (ADS)

    Martins Silva, H.; Abreu Da Silva, I.; Ronchetti, F.; Telesca, A.; Maidantchik, C.

    2015-12-01

    ALICE (A Large Ion Collider Experiment) is an experiment at the CERN LHC (Large Hadron Collider) studying the physics of strongly interacting matter and the quark-gluon plasma. The experiment operation requires a 24 hours a day and 7 days a week shift crew at the experimental site, composed by the ALICE collaboration members. Shift duties are calculated for each institute according to their correlated members. In order to ensure the full coverage of the experiment operation as well as its good quality, the ALICE Shift Accounting Management System (SAMS) is used to manage the shift bookings as well as the needed training. ALICE SAMS is the result of a joint effort between the Federal University of Rio de Janeiro (UFRJ) and the ALICE Collaboration. The Glance technology, developed by the UFRJ and the ATLAS experiment, sits at the basis of the system as an intermediate layer isolating the particularities of the databases. In this paper, we describe the ALICE SAMS development process and functionalities. The database has been modelled according to the collaboration needs and is fully integrated with the ALICE Collaboration repository to access members information and respectively roles and activities. Run, period and training coordinators can manage their subsystem operation and ensure an efficient personnel management. Members of the ALICE collaboration can book shifts and on-call according to pre-defined rights. ALICE SAMS features a user profile containing all the statistics and user contact information as well as the Institutes profile. Both the user and institute profiles are public (within the scope of the collaboration) and show the credit balance in real time. A shift calendar allows the Run Coordinator to plan data taking periods in terms of which subsystems shifts are enabled or disabled and on-call responsible people and slots. An overview display presents the shift crew present in the control room and allows the Run Coordination team to confirm the presence

  19. Genetic characterization of senescence-accelerated mouse (SAM).

    PubMed

    Higuchi, K

    1997-01-01

    The Senescence-Accelerated Mouse (SAM) strains are unique and appropriate models for genetic studies on aging because the SAMP strains have an "accelerated senescence" phenotype for which the SAMR strains are controls, and each SAMP strain has a strain-specific age-associated disorder. Furthermore, because they have gone through sufficient generations of sister-brother mating, they can be considered inbred strains, which can be analyzed genetically. There are now 11 SAMP strains and 3 SAMR strains descended from the progenitor litters. Analysis with the Gompertz function shows that the SAMP strains have the same initial mortality rate (IMR) as the SAMR strains but a shorter mortality rate doubling time (MRDT), presumably due to genes that accelerated the rate of senescence in the SAMP strains. This accelerated senescence may also occur in cultured fibroblast-like cells. We performed molecular genetic characterization of all the SAM strains to acquire a base of genetic information from which we could develop hypotheses on the mechanism of development of SAM strains and genetic factors that contribute to accelerated senescence. PMID:9088910

  20. Basis for ligand discrimination between ON and OFF state riboswitch conformations: the case of the SAM-I riboswitch.

    PubMed

    Boyapati, Vamsi Krishna; Huang, Wei; Spedale, Jessica; Aboul-Ela, Fareed

    2012-06-01

    Riboswitches are RNA elements that bind to effector ligands and control gene expression. Most consist of two domains. S-Adenosyl Methionine (SAM) binds the aptamer domain of the SAM-I riboswitch and induces conformational changes in the expression domain to form an intrinsic terminator (transcription OFF state). Without SAM the riboswitch forms the transcription ON state, allowing read-through transcription. The mechanistic link between the SAM/aptamer recognition event and subsequent secondary structure rearrangement by the riboswitch is unclear. We probed for those structural features of the Bacillus subtilis yitJ SAM-I riboswitch responsible for discrimination between the ON and OFF states by SAM. We designed SAM-I riboswitch RNA segments forming "hybrid" structures of the ON and OFF states. The choice of segment constrains the formation of a partial P1 helix, characteristic of the OFF state, together with a partial antiterminator (AT) helix, characteristic of the ON state. For most choices of P1 vs. AT helix lengths, SAM binds with micromolar affinity according to equilibrium dialysis. Mutational analysis and in-line probing confirm that the mode of SAM binding by hybrid structures is similar to that of the aptamer. Altogether, binding measurements and in-line probing are consistent with the hypothesis that when SAM is present, stacking interactions with the AT helix stabilize a partially formed P1 helix in the hybrids. Molecular modeling indicates that continuous stacking between the P1 and the AT helices is plausible with SAM bound. Our findings raise the possibility that conformational intermediates may play a role in ligand-induced aptamer folding.

  1. The Impact of a Ligand Binding on Strand Migration in the SAM-I Riboswitch

    PubMed Central

    Huang, Wei; Kim, Joohyun; Jha, Shantenu; Aboul-ela, Fareed

    2013-01-01

    Riboswitches sense cellular concentrations of small molecules and use this information to adjust synthesis rates of related metabolites. Riboswitches include an aptamer domain to detect the ligand and an expression platform to control gene expression. Previous structural studies of riboswitches largely focused on aptamers, truncating the expression domain to suppress conformational switching. To link ligand/aptamer binding to conformational switching, we constructed models of an S-adenosyl methionine (SAM)-I riboswitch RNA segment incorporating elements of the expression platform, allowing formation of an antiterminator (AT) helix. Using Anton, a computer specially developed for long timescale Molecular Dynamics (MD), we simulated an extended (three microseconds) MD trajectory with SAM bound to a modeled riboswitch RNA segment. Remarkably, we observed a strand migration, converting three base pairs from an antiterminator (AT) helix, characteristic of the transcription ON state, to a P1 helix, characteristic of the OFF state. This conformational switching towards the OFF state is observed only in the presence of SAM. Among seven extended trajectories with three starting structures, the presence of SAM enhances the trend towards the OFF state for two out of three starting structures tested. Our simulation provides a visual demonstration of how a small molecule (<500 MW) binding to a limited surface can trigger a large scale conformational rearrangement in a 40 kDa RNA by perturbing the Free Energy Landscape. Such a mechanism can explain minimal requirements for SAM binding and transcription termination for SAM-I riboswitches previously reported experimentally. PMID:23704854

  2. Validation of SAM 2 and SAGE satellite

    NASA Technical Reports Server (NTRS)

    Kent, G. S.; Wang, P.-H.; Farrukh, U. O.; Yue, G. K.

    1987-01-01

    Presented are the results of a validation study of data obtained by the Stratospheric Aerosol and Gas Experiment I (SAGE I) and Stratospheric Aerosol Measurement II (SAM II) satellite experiments. The study includes the entire SAGE I data set (February 1979 - November 1981) and the first four and one-half years of SAM II data (October 1978 - February 1983). These data sets have been validated by their use in the analysis of dynamical, physical and chemical processes in the stratosphere. They have been compared with other existing data sets and the SAGE I and SAM II data sets intercompared where possible. The study has shown the data to be of great value in the study of the climatological behavior of stratospheric aerosols and ozone. Several scientific publications and user-oriented data summaries have appeared as a result of the work carried out under this contract.

  3. Sam, Brookhaven, and the Physical Review

    NASA Astrophysics Data System (ADS)

    Blume, Martin

    2010-03-01

    Sam Goudsmit came to Brookhaven National Laboratory in 1948, just after the first year of operation of the new institution, and after a year of his postwar appointment as Professor of Physics at Northwestern University. He was named an associate editor of the Physical Review at that time, under the then Managing Editor John T. Tate of the University of Minnesota. Tate had been Editor since 1926, and had presided over the growth of Physical Review to leadership of publication in the world of physics. Tate died in 1950, and after a search under an interim Editor Sam was, in 1951, named Managing Editor. In 1952 he became Chair of the Brookhaven Physics Department, founded Physical Review Letters, and served as department chair until 1960, when he stepped down but remained an Associate Chair. I will discuss my own interactions with Sam during this later period, when I learned of his many faceted talents and accomplishments.

  4. Modulation of Conformational Equilibria in the S-Adenosylmethionine (SAM) II Riboswitch by SAM, Mg(2+), and Trimethylamine N-Oxide.

    PubMed

    McPhie, Peter; Brown, Patrick; Chen, Bin; Dayie, Theodore K; Minton, Allen P

    2016-09-13

    The dependence of the conformation of the S-adenosylmethionine (SAM) II riboswitch on the concentration of added Mg(2+) ions and SAM, individually and in mixtures, was monitored by circular dichroism (CD) spectroscopy and by measurement of the diffusion coefficient. The results are analyzed in the context of two complementary quantitative models, both of which are consistent with a single underlying physical model. Magnesium binding sites in the open state have an affinity on average higher than the affinity of those in the compact state, but formation of the compact state is accompanied by an increase in the number of binding sites. Consequently, at low Mg(2+) concentrations, Mg(2+) binds preferentially to the open state, favoring its formation, but at high concentrations, Mg(2+) binds preferentially to the compact state. The affinity of the riboswitch for SAM increases drastically with an increased level of binding of Mg(2+) to the compact pseudoknot conformation. The effect of increasing concentrations of trimethylamine N-oxide (TMAO), a well-studied molecular crowding agent, on the conformation of the riboswitch and its affinity for SAM were also monitored by CD spectroscopy and measurement of diffusion. In the absence of added Mg(2+), high concentrations of TMAO were found to induce a conformational change compatible with the formation of the pseudoknot form but have only a small effect on the affinity of the RNA for SAM. PMID:27552169

  5. Demographic Trends and Advocacy Experiences of Gay-Straight Alliance Advisors

    ERIC Educational Resources Information Center

    Graybill, Emily C.; Varjas, Kris; Meyers, Joel; Dever, Bridget V.; Greenberg, Daphne; Roach, Andrew T.; Morillas, Catalina

    2015-01-01

    Using an ecological model, the individual-, school-, and sociocultural-level characteristics that affect gay-straight alliance (GSA) advisors were examined in the current study. The formation of GSAs has been one way that schools have sought to improve the school climate for lesbian, gay, bisexual, and transgender (LGBT) youth. Limited information…

  6. Empowering Hispanic Students through Student Organizations: Competencies for Faculty Advisors.

    ERIC Educational Resources Information Center

    Delgado-Romero, Edward A.; Hernandez, Carlos A.

    2002-01-01

    Presents preliminary competencies for faculty advisors of Hispanic student organizations; the competencies are presented, defined, and illustrated with examples. Concludes with implications for research. (EV)

  7. Genetic typing of the senescence-accelerated mouse (SAM) strains with microsatellite markers.

    PubMed

    Xia, C; Higuchi, K; Shimizu, M; Matsushita, T; Kogishi, K; Wang, J; Chiba, T; Festing, M F; Hosokawa, M

    1999-03-01

    The Senescence-Accelerated Mouse (SAM) strains constitute a murine model of accelerated senescence originating from the ancestral AKR/J strains and consist of nine senescence-prone (SAMP) strains and four senescence-resistant (SAMR) strains. The chromosomes (Chrs) of the SAM strains were typed with 581 microsatellite markers amplified by PCR, and the fundamental genetic information of the SAM strains was obtained. One-third of the examined markers displayed polymorphism among the strains, and only two alleles were detected in almost all loci among the SAM and AKR/J strains. However, in 12 loci (5.6% of total 215 polymorphic markers), the third allele was detected among the SAM strains. The genetic typing and developmental history suggested that the SAM strains were related inbred strains developed by the accidental crossing between the AKR/J strain and other unknown strain(s). Comparison of the distribution of the loci in the SAMP and the SAMR series revealed notable differences in the four regions on Chrs 4, 14, 16, and 17. This indicated that some of these chromosomal sites might contain the genes responsible for accelerated senescence in the SAMP series. PMID:10051317

  8. Discrimination between closely related cellular metabolites by the SAM-I riboswitch.

    PubMed

    Montange, Rebecca K; Mondragón, Estefanía; van Tyne, Daria; Garst, Andrew D; Ceres, Pablo; Batey, Robert T

    2010-02-26

    The SAM-I riboswitch is a cis-acting element of genetic control found in bacterial mRNAs that specifically binds S-adenosylmethionine (SAM). We previously determined the 2.9-A X-ray crystal structure of the effector-binding domain of this RNA element, revealing details of RNA-ligand recognition. To improve this structure, variations were made to the RNA sequence to alter lattice contacts, resulting in a 0.5-A improvement in crystallographic resolution and allowing for a more accurate refinement of the crystallographic model. The basis for SAM specificity was addressed by a structural analysis of the RNA complexed to S-adenosylhomocysteine (SAH) and sinefungin and by measuring the affinity of SAM and SAH for a series of mutants using isothermal titration calorimetry. These data illustrate the importance of two universally conserved base pairs in the RNA that form electrostatic interactions with the positively charged sulfonium group of SAM, thereby providing a basis for discrimination between SAM and SAH. PMID:20006621

  9. Discrimination between Closely Related Cellular Metabolites by the SAM-I Riboswitch

    PubMed Central

    Montange, Rebecca K.; Mondragón, Estefanía; van Tyne, Daria; Garst, Andrew D.; Ceres, Pablo; Batey, Robert T.

    2009-01-01

    The SAM-I riboswitch is a cis-acting element of genetic control found in bacterial mRNAs that specifically binds S-adenosylmethionine (SAM). We previously determined the 2.9-Å X-ray crystal structure of the effector-binding domain of this RNA element, revealing details of RNA–ligand recognition. To improve this structure, variations were made to the RNA sequence to alter lattice contacts, resulting in a 0.5-Å improvement in crystallographic resolution and allowing for a more accurate refinement of the crystallographic model. The basis for SAM specificity was addressed by a structural analysis of the RNA complexed to S-adenosylhomocysteine (SAH) and sinefungin and by measuring the affinity of SAM and SAH for a series of mutants using isothermal titration calorimetry. These data illustrate the importance of two universally conserved base pairs in the RNA that form electrostatic interactions with the positively charged sulfonium group of SAM, thereby providing a basis for discrimination between SAM and SAH. PMID:20006621

  10. Discrimination between Closely Related Cellular Metabolites by the SAM-I Riboswitch

    SciTech Connect

    Montange, R.; Mondragon, E; van Tyne, D; Garst, A; Ceres, P; Batey, R

    2010-01-01

    The SAM-I riboswitch is a cis-acting element of genetic control found in bacterial mRNAs that specifically binds S-adenosylmethionine (SAM). We previously determined the 2.9-{angstrom} X-ray crystal structure of the effector-binding domain of this RNA element, revealing details of RNA-ligand recognition. To improve this structure, variations were made to the RNA sequence to alter lattice contacts, resulting in a 0.5-{angstrom} improvement in crystallographic resolution and allowing for a more accurate refinement of the crystallographic model. The basis for SAM specificity was addressed by a structural analysis of the RNA complexed to S-adenosylhomocysteine (SAH) and sinefungin and by measuring the affinity of SAM and SAH for a series of mutants using isothermal titration calorimetry. These data illustrate the importance of two universally conserved base pairs in the RNA that form electrostatic interactions with the positively charged sulfonium group of SAM, thereby providing a basis for discrimination between SAM and SAH.

  11. Emerging themes in radical SAM chemistry

    PubMed Central

    Shisler, Krista A; Broderick, Joan B

    2014-01-01

    Enzymes in the radical SAM (RS) superfamily catalyze a wide variety of reactions through unique radical chemistry. The characteristic markers of the superfamily include a [4Fe–4S] cluster coordinated to the protein via a cysteine triad motif, typically CX3CX2C, with the fourth iron coordinated by S-adenosylmethionine (SAM). The SAM serves as a precursor for a 5′-deoxyadenosyl radical, the central intermediate in nearly all RS enzymes studied to date. The SAM-bound [4Fe–4S] cluster is located within a partial or full triosephosphate isomerase (TIM) barrel where the radical chemistry occurs protected from the surroundings. In addition to the TIM barrel and a RS [4Fe–4S] cluster, many members of the superfamily contain additional domains and/or additional Fe–S clusters. Recently characterized superfamily members are providing new examples of the remarkable range of reactions that can be catalyzed, as well as new structural and mechanistic insights into these fascinating reactions. PMID:23141873

  12. Sam's Journey to "Reach for the Stars"

    ERIC Educational Resources Information Center

    Mayer, Sue

    2007-01-01

    In this article, the author shares her experiences as a parent of a child with Down syndrome. Although her son Sam's first years were filled with numerous hospitalizations and visits to pediatricians, which she feared would further delay his development, she soon discovered an organization known as the National Association of Child Development…

  13. Structural Basis for Methyl Transfer by a Radical SAM Enzyme

    SciTech Connect

    Boal, Amie K.; Grove, Tyler L.; McLaughlin, Monica I.; Yennawar, Neela H.; Booker, Squire J.; Rosenzweig, Amy C.

    2014-10-02

    The radical S-adenosyl-l-methionine (SAM) enzymes RlmN and Cfr methylate 23S ribosomal RNA, modifying the C2 or C8 position of adenosine 2503. The methyl groups are installed by a two-step sequence involving initial methylation of a conserved Cys residue (RlmN Cys{sup 355}) by SAM. Methyl transfer to the substrate requires reductive cleavage of a second equivalent of SAM. Crystal structures of RlmN and RlmN with SAM show that a single molecule of SAM coordinates the [4Fe-4S] cluster. Residue Cys{sup 355} is S-methylated and located proximal to the SAM methyl group, suggesting the SAM that is involved in the initial methyl transfer binds at the same site. Thus, RlmN accomplishes its complex reaction with structural economy, harnessing the two most important reactivities of SAM within a single site.

  14. SAM II Data and Information (HDF-EOS)

    Atmospheric Science Data Center

    2016-09-01

    SAM II Data and Information (HDF-EOS) Data obtained from the Stratospheric Aerosol Measurement (SAM) II instrument, ... Guide Documents:  Project Guide Data Set Guide Readme Files:  Data Set (Text file) ...

  15. 17 CFR 3.10 - Registration of futures commission merchants, introducing brokers, commodity trading advisors...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... commission merchants, introducing brokers, commodity trading advisors, commodity pool operators and leverage..., commodity trading advisors, commodity pool operators and leverage transaction merchants. (a) Application for... futures commission merchant, introducing broker, commodity trading advisor, commodity pool operator...

  16. Characterization of flourocarbon SAM coated MEMS tribogauge

    NASA Astrophysics Data System (ADS)

    Vijayasai, Ashwin; Ramachandran, Gautham; Sivakumar, Ganapathy; Anderson, Charlie; Gale, Richard; Dallas, Tim

    2012-03-01

    A MEMS tribogauge was used for on-chip and in-situ characterization of nano-tribological phenomena (stiction, friction, and wear of coated polysilicon surfaces). The device was fabricated using the SUMMiT-V process. Measurements were made on sidewall surfaces on the polysilicon-3 layer. The device consists of two orthogonally positioned comb-drive assemblies that are used for both actuation and sensing. One assembly is used to apply a normal load (Fn) to contacting surface, while the other induces a tangential load (FT). Precise position control is tracked by employing a LabVIEW controlled AD7747 capacitance sense mechanism. The resolution of the characterization apparatus is +/-10nm. Three MEMS tribogauge devices are tested; two of them have a chemisorbed layer of self-assembled monolayer (SAM) coatings and one with no SAM coating. The two types of SAM coatings are FOTS and 'Sandia vapor-SAM' (SVSAM). The tribogauge with no FSAM coating is either UV-Ozone or 'air plasma' treated to remove organic contaminants leaving behind -OH bonds on top of the MEMS surface (native oxide, SiO2). Characterization using the tribogauge for each coating type includes: measurement of baseline stiction force [see manuscript], static and dynamic coefficient of friction [see manuscript], induced stiction force calculated after specific load cycles [see manuscript]. Experiments showed that the induced stiction force increases in proportion to the increase in the number of load cycles, indicating degradation of the FSAM coating and topographical changes to the interacting surfaces. The UV-Ozone /air plasma treated pristine tribogauge was used to measure the stiction force of the device with no SAM coating [see manuscript].

  17. 77 FR 50493 - Sam Rayburn Dam Project Power Rate

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-21

    ... Southwestern Power Administration Sam Rayburn Dam Project Power Rate AGENCY: Southwestern Power Administration, DOE. ACTION: Notice of proposed extension. SUMMARY: The current Sam Rayburn Dam Project rate was... 46 microwave and VHF radio sites. Costs associated ] with the Sam Rayburn and Robert D. Willis...

  18. Expanding Radical SAM Chemistry by Using Radical Addition Reactions and SAM Analogues.

    PubMed

    Ji, Xinjian; Li, Yongzhen; Xie, Liqi; Lu, Haojie; Ding, Wei; Zhang, Qi

    2016-09-19

    Radical S-adenosyl-l-methionine (SAM) enzymes utilize a [4Fe-4S] cluster to bind SAM and reductively cleave its carbon-sulfur bond to produce a highly reactive 5'-deoxyadenosyl (dAdo) radical. In almost all cases, the dAdo radical abstracts a hydrogen atom from the substrates or from enzymes, thereby initiating a highly diverse array of reactions. Herein, we report a change of the dAdo radical-based chemistry from hydrogen abstraction to radical addition in the reaction of the radical SAM enzyme NosL. This change was achieved by using a substrate analogue containing an olefin moiety. We also showed that two SAM analogues containing different nucleoside functionalities initiate the radical-based reactions with high efficiencies. The radical adduct with the olefin produced in the reaction was found to undergo two divergent reactions, and the mechanistic insights into this process were investigated in detail. Our study demonstrates a promising strategy in expanding radical SAM chemistry, providing an effective way to access nucleoside-containing compounds by using radical SAM-dependent reactions. PMID:27573794

  19. Expanding Radical SAM Chemistry by Using Radical Addition Reactions and SAM Analogues.

    PubMed

    Ji, Xinjian; Li, Yongzhen; Xie, Liqi; Lu, Haojie; Ding, Wei; Zhang, Qi

    2016-09-19

    Radical S-adenosyl-l-methionine (SAM) enzymes utilize a [4Fe-4S] cluster to bind SAM and reductively cleave its carbon-sulfur bond to produce a highly reactive 5'-deoxyadenosyl (dAdo) radical. In almost all cases, the dAdo radical abstracts a hydrogen atom from the substrates or from enzymes, thereby initiating a highly diverse array of reactions. Herein, we report a change of the dAdo radical-based chemistry from hydrogen abstraction to radical addition in the reaction of the radical SAM enzyme NosL. This change was achieved by using a substrate analogue containing an olefin moiety. We also showed that two SAM analogues containing different nucleoside functionalities initiate the radical-based reactions with high efficiencies. The radical adduct with the olefin produced in the reaction was found to undergo two divergent reactions, and the mechanistic insights into this process were investigated in detail. Our study demonstrates a promising strategy in expanding radical SAM chemistry, providing an effective way to access nucleoside-containing compounds by using radical SAM-dependent reactions.

  20. Microskills: Advisor Behaviors that Improve Communication with Advisees

    ERIC Educational Resources Information Center

    Barnett, Susan; Roach, Scott; Smith, Martha

    2006-01-01

    In writing this article, we wanted to provide advisors practical means with which to optimize their relationships with advisees. First, we describe behavioral micro-skills that can be used by advisors to enhance advising sessions. Attending behaviors (eye contact vocal cadence and tone, verbal tracking, body language) and listening skills (asking…

  1. The Teacher Advisor Program: An Innovative Approach to School Guidance.

    ERIC Educational Resources Information Center

    Myrick, Robert D.; And Others

    The Teacher Advisor Program (TAP) is an innovative developmental approach to counseling that directly involves teachers as advisors to 18 to 20 students, with whom they meet both individually and in group sessions. An introductory chapter that cites the need for a developmental approach to counseling in contemporary schools and the potential role…

  2. Five Possible Work Profiles for Full-Time Academic Advisors

    ERIC Educational Resources Information Center

    Steele, George

    2006-01-01

    Five potential work profiles for full-time academic advisors, based on the impact of technology, are proposed. The forces accelerating the impact of technology are identified, and the impact of emerging technologies on full-time advisor practice is discussed.

  3. Academic Advisors of Military and Student Veterans: An Ethnographic Study

    ERIC Educational Resources Information Center

    Miller, Michelle A.

    2015-01-01

    With the introduction of the Post-9/11 GI Bill, there is an influx of active-duty military and student veterans enrolling in postsecondary and graduate-level education. The role of an academic advisor increases significantly with this influx of enrollment. The purpose of this study was to determine how a graduate-level academic advisor perceives…

  4. A study on optimization of hybrid drive train using Advanced Vehicle Simulator (ADVISOR)

    NASA Astrophysics Data System (ADS)

    Same, Adam; Stipe, Alex; Grossman, David; Park, Jae Wan

    This study investigates the advantages and disadvantages of three hybrid drive train configurations: series, parallel, and "through-the-ground" parallel. Power flow simulations are conducted with the MATLAB/Simulink-based software ADVISOR. These simulations are then applied in an application for the UC Davis SAE Formula Hybrid vehicle. ADVISOR performs simulation calculations for vehicle position using a combined backward/forward method. These simulations are used to study how efficiency and agility are affected by the motor, fuel converter, and hybrid configuration. Three different vehicle models are developed to optimize the drive train of a vehicle for three stages of the SAE Formula Hybrid competition: autocross, endurance, and acceleration. Input cycles are created based on rough estimates of track geometry. The output from these ADVISOR simulations is a series of plots of velocity profile and energy storage State of Charge that provide a good estimate of how the Formula Hybrid vehicle will perform on the given course. The most noticeable discrepancy between the input cycle and the actual velocity profile of the vehicle occurs during deceleration. A weighted ranking system is developed to organize the simulation results and to determine the best drive train configuration for the Formula Hybrid vehicle. Results show that the through-the-ground parallel configuration with front-mounted motors achieves an optimal balance of efficiency, simplicity, and cost. ADVISOR is proven to be a useful tool for vehicle power train design for the SAE Formula Hybrid competition. This vehicle model based on ADVISOR simulation is applicable to various studies concerning performance and efficiency of hybrid drive trains.

  5. Are We in This Together? Partnerships between Academic Advisors and Advising Administrators and Their Relation to Advisor Job Satisfaction

    ERIC Educational Resources Information Center

    Chabinak, Sarah Elizabeth

    2010-01-01

    Scope and Method of Study: This study examined the relationship between professional academic advisors' job satisfaction and perceptions of respect by administration, involvement in decision making, and autonomy. 290 professional academic advisors from 41 public FT4/MS/HTI Carnegie classified institutions participated in the quantitative study…

  6. Spot and Runway Departure Advisor (SARDA)

    NASA Technical Reports Server (NTRS)

    Jung, Yoon

    2016-01-01

    Spot and Runway Departure Advisor (SARDA) is a decision support tool to assist airline ramp controllers and ATC tower controllers to manage traffic on the airport surface to significantly improve efficiency and predictability in surface operations. The core function of the tool is the runway scheduler which generates an optimal solution for runway sequence and schedule of departure aircraft, which would minimize system delay and maximize runway throughput. The presentation also discusses the latest status of NASA's current surface research through a collaboration with an airline partner, where a tool is developed for airline ramp operators to assist departure pushback operations. The presentation describes the concept of the SARDA tool and results from human-in-the-loop simulations conducted in 2012 for Dallas-Ft. Worth International Airport and 2014 for Charlotte airport ramp tower.

  7. An Aggregation Advisor for Ligand Discovery.

    PubMed

    Irwin, John J; Duan, Da; Torosyan, Hayarpi; Doak, Allison K; Ziebart, Kristin T; Sterling, Teague; Tumanian, Gurgen; Shoichet, Brian K

    2015-09-10

    Colloidal aggregation of organic molecules is the dominant mechanism for artifactual inhibition of proteins, and controls against it are widely deployed. Notwithstanding an increasingly detailed understanding of this phenomenon, a method to reliably predict aggregation has remained elusive. Correspondingly, active molecules that act via aggregation continue to be found in early discovery campaigns and remain common in the literature. Over the past decade, over 12 thousand aggregating organic molecules have been identified, potentially enabling a precedent-based approach to match known aggregators with new molecules that may be expected to aggregate and lead to artifacts. We investigate an approach that uses lipophilicity, affinity, and similarity to known aggregators to advise on the likelihood that a candidate compound is an aggregator. In prospective experimental testing, five of seven new molecules with Tanimoto coefficients (Tc's) between 0.95 and 0.99 to known aggregators aggregated at relevant concentrations. Ten of 19 with Tc's between 0.94 and 0.90 and three of seven with Tc's between 0.89 and 0.85 also aggregated. Another three of the predicted compounds aggregated at higher concentrations. This method finds that 61 827 or 5.1% of the ligands acting in the 0.1 to 10 μM range in the medicinal chemistry literature are at least 85% similar to a known aggregator with these physical properties and may aggregate at relevant concentrations. Intriguingly, only 0.73% of all drug-like commercially available compounds resemble the known aggregators, suggesting that colloidal aggregators are enriched in the literature. As a percentage of the literature, aggregator-like compounds have increased 9-fold since 1995, partly reflecting the advent of high-throughput and virtual screens against molecular targets. Emerging from this study is an aggregator advisor database and tool ( http://advisor.bkslab.org ), free to the community, that may help distinguish between

  8. Ambient STM Study of Long Chain Hydrophobic Thiol SAMs on HOPG and Au-capped HOPG

    NASA Astrophysics Data System (ADS)

    Maurer, Mackenzie; Bowers, Alexis; Senevirathne, Indrajith

    2015-03-01

    Thiol-based self-assembled monolayers (SAM) surfaces are ubiquitous in many device applications including sensor engineering. The conductivity characteristics and surface molecular structure and orientation of these SAMs are important as physiochemical properties are dependent on the surface arrangement. This study attempts to quantify and model long chain -R terminated (hydrophobic) 1-dodecanethiol on both Highly Oriented Pyrolytic Graphite (HOPG) and thermally annealed Au thin films capped on HOPG substrates. Study uses Scanning Tunneling Microscopy (STM), contact angle measurements and reflective spectroscopy to assess the SAM layered surface. 5mM concentrated solutions of 1-dodecanethiol dissolved in 200 proof ethanol were prepared for the self-assembly process. These solutions were used in developing SAMs on freshly cleaved HOPG. Au thin layers were sputter deposited on HOPG and subsequently annealed. Data indicated Au deposition changes the surface consistency. Uniqueness of this study is the ambient conditions under which data was obtained. Surface structure, consistency and possible thiol molecular arrangement of the SAM layer will be discussed.

  9. Obituary: Sam Roweis (1972-2010)

    NASA Astrophysics Data System (ADS)

    Hogg, David

    2011-12-01

    Computer scientist and statistical astronomer Sam Roweis took his own life in New York City on 2010 January 12. He was a brilliant and accomplished researcher in the field of machine learning, and a strong advocate for the use of computational statistics for automating discovery and scientific data analysis. He made several important contributions to astronomy and was working on adaptive astronomical data analysis at the time of his death. Roweis obtained his PhD in 1999 from the California Institute of Technology, where he worked on a remarkable range of subjects, including DNA computing, modeling of dynamical systems, signal processing, and speech recognition. During this time he unified and clarified some of the most important data analysis techniques, including Principal Component Analysis, Hidden Markov Models, and Expectation Maximization. His work was aimed at making data analysis and modeling faster, but also better justified scientifically. The last years of his PhD were spent in Princeton NJ, where he came in contact with a young generation of cosmologists thinking about microwave background and large-scale structure data. In a postdoc at University College London, Roweis co-created the Locally Linear Embedding (LLE) algorithm; a simple but flexible technique for mapping a large data set onto a low-dimensional manifold. The LLE paper obtained more than 2700 citations in 9 years, launched a new sub-field of machine learning known as "manifold learning," and inspired work in data visualization, search, and applied mathematics. In 2001, Roweis took a faculty job at the University of Toronto Computer Science Department. He continued working on data analysis methods that have probabilistic interpretation and therefore scientific applicability, but at the same time have good performance on large data sets. He was awarded a Sloan Fellowship, a Canada Research Chair, and a fellowship of the Canadian Institute for Advanced Research, among other honors and awards

  10. Senescence-accelerated mouse (SAM): a biogerontological resource in aging research.

    PubMed

    Takeda, T

    1999-01-01

    The senescence-accelerated mouse (SAM), consisting of 14 senescence-prone inbred strains (SAMP) and 4 senescence-resistant inbred strains (SAMR) has been under development since 1970 through the selective inbreeding of AKR/J strain mice donated by the Jackson laboratory in 1968, based on the data of the grading score of senescence, life span, and pathologic phenotypes. The characteristic feature of aging common to all SAMP and SAMR mice is accelerated senescence and normal aging, respectively. Furthermore, SAMP and SAMR strains manifest various pathobiological phenotypes which include such neurobiological phenotypes as deficits in learning and memory, emotional disorders, abnormal circadian rhythms, brain atrophy, hearing impairment, etc., and are often characteristic enough to differentiate the strains. Various efforts are currently being made using the SAM model to clarify the underlying mechanisms in accelerated senescence as well as the etiopathogenic mechanisms in age-associated pathobiologies. Genetic background and significance of SAM development are discussed. PMID:10537019

  11. AFM-assisted fabrication of thiol SAM pattern with alternating quantified surface potential

    PubMed Central

    2011-01-01

    Thiol self-assembled monolayers (SAMs) are widely used in many nano- and bio-technology applications. We report a new approach to create and characterize a thiol SAMs micropattern with alternating charges on a flat gold-coated substrate using atomic force microscopy (AFM) and Kelvin probe force microscopy (KPFM). We produced SAMs-patterns made of alternating positively charged, negatively charged, and hydrophobic-terminated thiols by an automated AFM-assisted manipulation, or nanografting. We show that these thiol patterns possess only small topographical differences as revealed by AFM, and distinguished differences in surface potential (20-50 mV), revealed by KPFM. The pattern can be helpful in the development of biosensor technologies, specifically for selective binding of biomolecules based on charge and hydrophobicity, and serve as a model for creating surfaces with quantified alternating surface potential distribution. PMID:21711703

  12. Princeton`s science advisors: A neural network

    SciTech Connect

    Rubenstein, D.

    1994-12-31

    Princeton`s Science Advisor Program aims to place a Science Advisor in every school - elementary through high school - in eight school districts, both urban and suburban, in New Jersey`s Mercer and Hunterdon counties. Each scientist visits his or her school regularly, getting to know the teachers and their needs. Each advisor is trained during a summer workshop and is supported throughout the year by a network of electronically linked scientists who are always on call. As front line activists, Advisors interpret and communicate the needs of teachers and their students, and then convert advice, information, and services from the network into usable form. Since the program is designed to be a true partnership between the schools and local scientists, teachers and their educational concerns are its driving force. Ultimately, the support and encouragement of teachers provide the energy and rationale for making the experiment a lasting success and for truly transforming the doing of science in the classroom.

  13. 75 FR 54465 - Temporary Registration of Municipal Advisors

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-08

    ..., committed by contract or other arrangement to support the payment of all or part of the obligations on the...--in the case of a sole proprietor, for example--a Social Security Number. If the municipal advisor...

  14. MMW, IR, and SAM signature collection

    NASA Astrophysics Data System (ADS)

    Reichstetter, Fred; Ward, Mary E.

    2002-08-01

    During the development of smart weapon's seeker/sensors, it is imperative to collect high quality signatures of the targets the system is intended to engage. These signatures are used to support algorithm development so the system can find and engage the targets of interest in the specific kill area on the target. Englin AFB FL is the AF development center for munitions; and in support of the development effort, the 46th Test Wing (46 TW) has initiated significant improvements in collection capabilities for signatures in the MMW, Infrared and Seismic, Acoustic and Magnetic (SAM) spectrum. Additionally, the Joint Munitions Test and Evaluation program office maintains a fleet of foreign ground vehicle targets used for such signature collection including items such as tanks, SCUD missile launchers, air defense units such as SA-06, SA-8, SA-13, and associated ground support trucks and general purpose vehicles. The major test facility includes a 300 ft tower used for mounting the instrumentation suite that currently includes, 10, 35 and 94 GHz MMW and 2-5(mu) and 8-12(mu) IR instrumentation systems. This facility has undergone major improvements in terms of background signature reduction, construction of a high bay building to house the turntable on which the targets are mounted, and an additional in- ground stationary turntable primarily for IR signature collection. Our experience using this facility to collect signatures for the smart weapons development community has confirmed a significant improvement in quality and efficiency. The need for the stationary turntable signature collection capability was driven by the requirements of the IR community who are interested in collecting signatures in clutter. This tends to be contrary to the MMW community that desires minimum background clutter. The resulting location, adjacent to the MMW tower, allows variations in the type and amount of clutter background that could be incorporated and also provides maximum utilization of

  15. Contextualizing Gay-Straight Alliances: Student, Advisor, and Structural Factors Related to Positive Youth Development Among Members

    PubMed Central

    Poteat, V. Paul; Yoshikawa, Hirokazu; Calzo, Jerel P.; Gray, Mary L.; DiGiovanni, Craig. D.; Lipkin, Arthur; Mundy-Shephard, Adrienne; Perrotti, Jeff; Scheer, Jillian R.; Shaw, Mathew P.

    2014-01-01

    Gay-Straight Alliances (GSAs) may promote resilience. Yet, what GSA components predict wellbeing? Among 146 youth and advisors in 13 GSAs (58% lesbian, gay, bisexual, or questioning; 64% white; 38% received free/reduced-cost lunch), student (demographics, victimization, attendance frequency, leadership, support, control), advisor (years served, training, control) and contextual factors (overall support or advocacy, outside support for the GSA) that predicted purpose, mastery, and self-esteem were tested. In multilevel models, GSA support predicted all outcomes. Racial/ethnic minority youth reported greater wellbeing, yet lower support. Youth in GSAs whose advisors served longer and perceived more control and were in more supportive school contexts reported healthier outcomes. GSA advocacy also predicted purpose. Ethnographic notes elucidated complex associations and variability in how GSAs operated. PMID:25176579

  16. Neal Lane: Confessions of a President's Science Advisor

    ScienceCinema

    Neal Lane

    2016-07-12

    Former science advisor to president Bill Clinton Neal Lane briefly reviews the history of the job of Science Advisor to the President and give some examples of issues he had to deal with when he was in that position, including climate change, stem cell research, the human genome, nanotechnology and research funding. He will also give his opinions about the present and future state of science in the U.S.

  17. Neal Lane: Confessions of a President's Science Advisor

    SciTech Connect

    Neal Lane

    2006-09-11

    Former science advisor to president Bill Clinton Neal Lane briefly reviews the history of the job of Science Advisor to the President and give some examples of issues he had to deal with when he was in that position, including climate change, stem cell research, the human genome, nanotechnology and research funding. He will also give his opinions about the present and future state of science in the U.S.

  18. An intelligent advisor for the design manager

    NASA Technical Reports Server (NTRS)

    Rogers, James L.; Padula, Sharon L.

    1989-01-01

    A design problem is viewed as a complex system divisible into modules. Before the design of a complex system can begin, much time and money are spent in determining the couplings among modules and the presence of iterative loops. This is important because the design manager must know how to group the modules into substems and how to assign subsystems to design teams so that changes in one subsystem will have predictable effects on other subsystems. Determining these subsystems is not an easy, straightforward process and often important couplings are overlooked. Moreover, the planning task must be repeated as new information becomes available or as the design specifications change. The purchase of this research effort is to develop a knowledge-based tool to act as an intelligent advisor for the design manager. This tool identifies the subsystems of a complex design problem, orders them into a well-structured format, and marks the couplings among the subsystems to facilitate the use of multilevel tools. The tool was tested in the decomposition of the COFS (Control of Flexible Structures) mast design which has about 50 modules. This test indicated that this type of approach could lead to a substantial savings by organizing and displaying a complex problem as a sequence of subsystems easily divisible among design teams.

  19. Experience with the shift technical advisor position

    SciTech Connect

    Melber, B.D.; Olson, J.; Schreiber, R.E.; Winges, L.

    1984-03-01

    The provision of engineering expertise on shift at commercial nuclear power plants has mainly taken the form of the Shift Technical Advisor (STA). This person, acting in a capacity that is part engineer and part operator, is expected to advise the operations crew in the event of an emergency and review plant operating experience during normal circumstances. The position was mandated by the Nuclear Regulatory Commission following the incident at Three Mile Island. This report expands on a growing body of knowledge regarding the effectiveness of the STA. The new data presented here come from interviews with plant personnel and utility officials from nine sites. Researchers from the Pacific Northwest Laboratory (PNL) interviewed plant personnel, including the STA and immediate management, the shift supervisor and management, the training department, and ancillary staff, all of whom affect the intended performance of the STA. The conclusions of the report are that the design of the STA position results in limited contribution during emergencies; more comprehensive ways should be sought to provide the variety and specificity of engineering expertise needed during such times.

  20. Halomethane production in plants: Structure of the biosynthetic SAM-dependent halide methyltransferase from Arabidopsis thaliana**

    PubMed Central

    Schmidberger, Jason W.; James, Agata B.; Edwards, Robert; Naismith, James H.; O’Hagan, David

    2012-01-01

    A product structure of the halomethane producing enzyme in plants (Arabidopsis thaliana) is reported and a model for presentation of chloride/bromide ion to the methyl group of S-adenosyl-L-methionine (SAM) is presented to rationalise nucleophilic halide attack for halomethane production, gaseous natural products that are produced globally. PMID:20376845

  1. The SAM domain of ANKS6 has different interacting partners and mutations can induce different cystic phenotypes.

    PubMed

    Bakey, Zeineb; Bihoreau, Marie-Thérèse; Piedagnel, Rémi; Delestré, Laure; Arnould, Catherine; de Villiers, Alexandre d'Hotman; Devuyst, Olivier; Hoffmann, Sigrid; Ronco, Pierre; Gauguier, Dominique; Lelongt, Brigitte

    2015-08-01

    The ankyrin repeat and sterile α motif (SAM) domain-containing six gene (Anks6) is a candidate for polycystic kidney disease (PKD). Originally identified in the PKD/Mhm(cy/+) rat model of PKD, the disease is caused by a mutation (R823W) in the SAM domain of the encoded protein. Recent studies support the etiological role of the ANKS6 SAM domain in human cystic diseases, but its function in kidney remains unknown. To investigate the role of ANKS6 in cyst formation, we screened an archive of N-ethyl-N-nitrosourea-treated mice and derived a strain carrying a missense mutation (I747N) within the SAM domain of ANKS6. This mutation is only six amino acids away from the PKD-causing mutation (R823W) in cy/+ rats. Evidence of renal cysts in these mice confirmed the crucial role of the SAM domain of ANKS6 in kidney function. Comparative phenotype analysis in cy/+ rats and our Anks6(I747N) mice further showed that the two models display noticeably different PKD phenotypes and that there is a defective interaction between ANKS6 with ANKS3 in the rat and between ANKS6 and BICC1 (bicaudal C homolog 1) in the mouse. Thus, our data demonstrate the importance of ANKS6 for kidney structure integrity and the essential mediating role of its SAM domain in the formation of protein complexes.

  2. Understanding the SAM influence on the South Pacific ENSO teleconnection

    NASA Astrophysics Data System (ADS)

    Fogt, Ryan L.; Bromwich, David H.; Hines, Keith M.

    2011-04-01

    The relationship between the El Niño Southern Oscillation (ENSO) and the Southern Hemisphere Annular Mode (SAM) is examined, with the goal of understanding how various strong SAM events modulate the ENSO teleconnection to the South Pacific (45°-70°S, 150°-70°W). The focus is on multi-month, multi-event variations during the last 50 years. A significant ( p < 0.10) relationship is observed, most marked during the austral summer and in the 1970s and 1990s. In most cases, the significant relationship is brought about by La Niña (El Niño) events occurring with positive (negative) phases of the SAM more often than expected by chance. The South Pacific teleconnection magnitude is found to be strongly dependent on the SAM phase. Only when ENSO events occur with a weak SAM or when a La Niña (El Niño) occurs with a positive (negative) SAM phase are significant South Pacific teleconnections found. This modulation in the South Pacific ENSO teleconnection is directly tied to the interaction of the anomalous ENSO and SAM transient eddy momentum fluxes. During La Niña/SAM+ and El Niño/SAM- combinations, the anomalous transient momentum fluxes in the Pacific act to reinforce the circulation anomalies in the midlatitudes, altering the circulation in such a way to maintain the ENSO teleconnections. In La Niña/SAM- and El Niño/SAM+ cases, the anomalous transient eddies oppose each other in the midlatitudes, overall acting to reduce the magnitude of the high latitude ENSO teleconnection.

  3. Inhibition of Sam68 triggers adipose tissue browning

    PubMed Central

    Zhou, Junlan; Cheng, Min; Boriboun, Chan; Ardehali, Mariam Mina; Jiang, Changfei; Liu, Qinghua; Han, Shuling; Goukassian, David A.; Tang, Yao-Liang; Zhao, Ting C.; Zhao, Ming; Cai, Lu; Richard, Stéphane; Kishore, Raj; Qin, Gangjian

    2015-01-01

    Obesity is associated with insulin resistance and type 2 diabetes; molecular mechanisms promoting energy expenditure may be utilized for effective therapy. Src-associated-in-mitosis-of-68kDa (Sam68) is potentially significant because knockout (KO) of Sam68 leads to markedly-reduced adiposity. Here we sought to determine the mechanism by which Sam68 regulates adiposity and energy homeostasis. We firstly found in Sam68-KO mice a significantly-reduced body weight with the difference explained entirely by decreased adiposity. Interestingly, these effects were not mediated by a difference in food intake, but rather associated with enhanced physical activity. When fed high-fat diet, Sam68-KO mice gained much lesser body weight and fat mass as compared to wild-type (WT) littermates and displayed an improved glucose and insulin tolerance. The brown adipose tissue (BAT), inguinal and epididymal depots are smaller and their adipocytes less hypertrophy in Sam68-KO mice than in WT littermates. The BAT of Sam68-KO mice exhibited reduced lipid stores and expressed higher levels of Ucp1 and key thermogenic and fatty-acid-oxidation genes. Similarly, depots of inguinal and epididymal white adipose tissue (WAT) in Sam68-KO mice appeared browner, their multilocular Ucp1-positive cells were much more abundant, and the expression of Ucp1, Cidea, Prdm16 and Ppargc1a genes was greater as compared to WT controls, suggesting that loss of Sam68 also promotes WAT browning. Furthermore, in all fat depots of Sam68-KO mice, the expression of M2 macrophage markers were upregulated and M1 markers downregulated. Thus Sam68 plays a crucial role in the control of thermogenesis and may be targeted to combat obesity and associated disorders. PMID:25934704

  4. Inhibition of Sam68 triggers adipose tissue browning.

    PubMed

    Zhou, Junlan; Cheng, Min; Boriboun, Chan; Ardehali, Mariam M; Jiang, Changfei; Liu, Qinghua; Han, Shuling; Goukassian, David A; Tang, Yao-Liang; Zhao, Ting C; Zhao, Ming; Cai, Lu; Richard, Stéphane; Kishore, Raj; Qin, Gangjian

    2015-06-01

    Obesity is associated with insulin resistance and type 2 diabetes; molecular mechanisms that promote energy expenditure can be utilized for effective therapy. Src-associated in mitosis of 68 kDa (Sam68) is potentially significant, because knockout (KO) of Sam68 leads to markedly reduced adiposity. In the present study, we sought to determine the mechanism by which Sam68 regulates adiposity and energy homeostasis. We first found that Sam68 KO mice have a significantly reduced body weight as compared to controls, and the difference is explained entirely by decreased adiposity. Interestingly, these effects were not mediated by a difference in food intake; rather, they were associated with enhanced physical activity. When they were fed a high-fat diet, Sam68 KO mice gained much less body weight and fat mass than their WT littermates did, and they displayed an improved glucose and insulin tolerance. In Sam68 KO mice, the brown adipose tissue (BAT), inguinal, and epididymal depots were smaller, and their adipocytes were less hypertrophied as compared to their WT littermates. The BAT of Sam68 KO mice exhibited reduced lipid stores and expressed higher levels of Ucp1 and key thermogenic and fatty acid oxidation genes. Similarly, depots of inguinal and epididymal white adipose tissue (WAT) in Sam68 KO mice appeared browner, their multilocular Ucp1-positive cells were much more abundant, and the expression of Ucp1, Cidea, Prdm16, and Ppargc1a genes was greater as compared to WT controls, which suggests that the loss of Sam68 also promotes WAT browning. Furthermore, in all of the fat depots of the Sam68 KO mice, the expression of M2 macrophage markers was up-regulated, and that of M1 markers was down-regulated. Thus, Sam68 plays a crucial role in controlling thermogenesis and may be targeted to combat obesity and associated disorders.

  5. Using SAM Assessment and Training for Office 2003

    ERIC Educational Resources Information Center

    Whittle, Gary

    2005-01-01

    This presentation will demonstrate the uses of SAM 2003 from Course Technology as a skills assessment and training software that is used via the Internet. Historically, testing in computer education has taken the form of pencil and paper or standardized testing. The actual computer skills of the student have not been properly assessed. With SAM,…

  6. [Anti-aging studies on the senescence accelerated mouse (SAM) strains].

    PubMed

    Takahashi, Ryoya

    2010-01-01

    Senescence accelerated mouse (SAM), a murine model of accelerated senescence, was established by Toshio Takeda and colleagues. SAM consists of series of SAMP (prone) and SAMR (resistant) lines. All SAMP lines (from SAMP1 to SAMP11) are characterized by accelerated accumulation of senile features, earlier onset and faster progress of age-associated pathological phenotypes, such as amyloidosis, impaired immune response, senile osteoporosis and deficits in learning and memory. These SAMP lines are useful for evaluation of putative anti-aging therapies. For example, SAMP1 line is used to study the anti-aging effect of the antioxidant containing foods and various anti-oxidants, such as coenzyme Q10, vitamin C, lycopene. SAMP8 line exhibiting an early onset of impaired learning and memory is often used for test strategies for therapeutic intervention of dementia of early onset. SAMP6 is used as an animal model for developing new strategies for the treatment of osteoporosis in humans. Various lines of SAM (P1, P6, P8, P10 and R1) are now commercially available for research. In this review, I will briefly introduce various usages of SAM in anti-aging research. PMID:20046059

  7. The SAM domain of polyhomeotic forms a helical polymer.

    PubMed

    Kim, Chongwoo A; Gingery, Mari; Pilpa, Rosemarie M; Bowie, James U

    2002-06-01

    The polycomb group (PcG) proteins are important in the maintenance of stable repression patterns during development. Several PcG members contain a protein protein interaction module called a SAM domain (also known as SPM, PNT and HLH). Here we report the high-resolution structure of the SAM domain of polyhomeotic (Ph). Ph-SAM forms a helical polymer structure, providing a likely mechanism for the extension of PcG complexes. The structure of the polymer resembles that formed by the SAM domain of another transcriptional repressor, TEL. The formation of these polymer structures by SAM domains in two divergent repressors suggests a conserved mode of repression involving a higher order chromatin structure.

  8. Student Activities in Meteorology (SAM), June 1994

    SciTech Connect

    Meier, B.L.; Passarelli, E.

    1994-06-01

    In an effort to inspire student interest in science and technology, scientists from the Forecast Systems Laboratory, a laboratory within the National Oceanic and Atmospheric Administration's (NOAA) Environmental Research Laboratories, and classroom teachers from the Boulder Valley School District collaborated to produce a series of classroom science activities on meteorology and atmospheric science. We call this series 'Student Activities in Meteorology,' or SAM. The goal is to provide activities that are interesting to students, and at the same time convenient and easy to use for teachers. The activity topics chosen are to incorporate trend setting scientific research and cutting edge technology. Several of the activities focus on the meteorological concerns of the Denver metropolitan area because many of NOAA's research labs are located in Boulder, where much of the research and testing for the region is performed. We believe that these activities are versatile and can be easily integrated into current science, environmental studies, health, social studies, and math curricula.

  9. Obituary: Sam Roweis (1972-2010)

    NASA Astrophysics Data System (ADS)

    Hogg, David

    2011-12-01

    Computer scientist and statistical astronomer Sam Roweis took his own life in New York City on 2010 January 12. He was a brilliant and accomplished researcher in the field of machine learning, and a strong advocate for the use of computational statistics for automating discovery and scientific data analysis. He made several important contributions to astronomy and was working on adaptive astronomical data analysis at the time of his death. Roweis obtained his PhD in 1999 from the California Institute of Technology, where he worked on a remarkable range of subjects, including DNA computing, modeling of dynamical systems, signal processing, and speech recognition. During this time he unified and clarified some of the most important data analysis techniques, including Principal Component Analysis, Hidden Markov Models, and Expectation Maximization. His work was aimed at making data analysis and modeling faster, but also better justified scientifically. The last years of his PhD were spent in Princeton NJ, where he came in contact with a young generation of cosmologists thinking about microwave background and large-scale structure data. In a postdoc at University College London, Roweis co-created the Locally Linear Embedding (LLE) algorithm; a simple but flexible technique for mapping a large data set onto a low-dimensional manifold. The LLE paper obtained more than 2700 citations in 9 years, launched a new sub-field of machine learning known as "manifold learning," and inspired work in data visualization, search, and applied mathematics. In 2001, Roweis took a faculty job at the University of Toronto Computer Science Department. He continued working on data analysis methods that have probabilistic interpretation and therefore scientific applicability, but at the same time have good performance on large data sets. He was awarded a Sloan Fellowship, a Canada Research Chair, and a fellowship of the Canadian Institute for Advanced Research, among other honors and awards

  10. Comparing Surfaces and Engineered Interfaces using Self-Assembled Monolayers (SAMs) and Injected SAMs Silanes

    SciTech Connect

    Morris, Mark J.; Simmons, Kevin L.

    2003-11-01

    The objective of this study was to show a comparison between property changes by formation of a self-assembled monolayer on the surface of PPG synthetic precipitated silica, which is a technique developed at PNNL, and by adding the SAMs silane chemical directly into the mixing bowl. These coatings have the potential to greatly increase the bond strength and enhance other properties between the particle and the rubber matrix of a rubber compound. Tensile testing measured peak stress and elongation at break. The increase in tensile strength shows how well the polymer-filler interfacial adhesion is doing. The study used five different SAM systems with a sulfur cured styrene butadiene rubber (SBR) tire rubber formulation. The three propylsilanes were propyl triethoxysilane, allyl triethoxysilane and 3-mercaptopropyl triethoxysilane. Five combinations of silanes were used in this study. The application of the silanes were 100% propyl triethoxy silane (100% Alkyl); a 10/90 mixture of allyl and propyl triethoxy silanes (10% vinyl/90% alkyl); a 50/50 mixture of the allyl and propyl (50% vinyl/50% alkyl); a 10/90mixture of 3-mercaptopropyl trimethoxysilane and propyl trimethoxysilane (10% mercaptan/90% alkyl) and lastly a 50/50 3-mercaptopropyl and propylsilanes (50% mercaptan/alkyl). The data not only shows improvement with SAMs, the peak stress data (ultimate strength) shows that the by changing the amount of silane content can change the physical properties

  11. Spectral mapping of morphological features on the moon with MGM and SAM

    NASA Astrophysics Data System (ADS)

    Kodikara, Gayantha R. L.; Champati ray, P. K.; Chauhan, Prakash; Chatterjee, R. S.

    2016-02-01

    Three types of morphological features observed in different lunar crustal terrains were studied and mapped using hyperspectral Moon mineralogy mapper (M3) data onboard Chandrayaan 1 mission in order to assess the utility of cascaded MGM-SAM spectral mixture modeling approach to characterize the surface materials, which may occur as mineral mixtures, at different topography of the lunar surface. Selected morphological features include: the impact melts in Orientale basin, sinuous rilles in Procellarum KREEP Terrane (PKT) and a rayed crater in Feldspathic Highland Terrane (FHT). Methodology involves extraction of spectrally pure pixels (endmembers) of the area using Pixel Purity Index (PPI), identification of mineralogy of the selected endmember spectrum using the Modified Gaussian Method (MGM) and mapping of mineralogically identified endmembers using the Spectral Angle Mapper (SAM) method. Mapping results demonstrate both the capabilities and the limitations of the MGM method of spectral deconvolution and the SAM method of spectral matching as effective tools for compositional characterizations of morphological features on the lunar surface. As a method of spectral deconvolution, MGM was able to identify and characterize both high- and low - Ca pyroxenes along with plagioclase feldspar. The Spectral Angle Mapper (SAM) was able to map identified mineral mixtures from MGM.

  12. SAM68 is a physiological regulator of SMN2 splicing in spinal muscular atrophy

    PubMed Central

    Pagliarini, Vittoria; Pelosi, Laura; Bustamante, Maria Blaire; Nobili, Annalisa; Berardinelli, Maria Grazia; D’Amelio, Marcello; Musarò, Antonio

    2015-01-01

    Spinal muscular atrophy (SMA) is a neurodegenerative disease caused by loss of motor neurons in patients with null mutations in the SMN1 gene. The almost identical SMN2 gene is unable to compensate for this deficiency because of the skipping of exon 7 during pre–messenger RNA (mRNA) processing. Although several splicing factors can modulate SMN2 splicing in vitro, the physiological regulators of this disease-causing event are unknown. We found that knockout of the splicing factor SAM68 partially rescued body weight and viability of SMAΔ7 mice. Ablation of SAM68 function promoted SMN2 splicing and expression in SMAΔ7 mice, correlating with amelioration of SMA-related defects in motor neurons and skeletal muscles. Mechanistically, SAM68 binds to SMN2 pre-mRNA, favoring recruitment of the splicing repressor hnRNP A1 and interfering with that of U2AF65 at the 3′ splice site of exon 7. These findings identify SAM68 as the first physiological regulator of SMN2 splicing in an SMA mouse model. PMID:26438828

  13. SAM Gcms Chromatography Performed at Mars : Elements of Interpretation

    NASA Astrophysics Data System (ADS)

    Szopa, C.; Coll, P. J.; Buch, A.; François, P.; Cabane, M.; Coscia, D.; Teinturier, S.; Navarro-Gonzalez, R.; Glavin, D. P.; Freissinet, C.; Mahaffy, P. R.

    2013-12-01

    The characterisation of the chemical and mineralogical composition of regolith samples collected with the Curiosity rover is a primary objective of the SAM experiment. These data should provide essential clues on the past habitability of Gale crater. Interpretation of the data collected after SAM pyrolysis evolved gas analysis (EGA) and gas chromatography mass spectrometry (GC-MS) experiments on the first soil samples collected by MSL at the Rocknest Aeolian Deposit in Gale Crater has been challenging due to the concomitant presence in the ovens of an oxychlorine phase present in the samples, and a derivatization agent coming from the SAM wet chemistry experiment (Glavin et al., 2013). Moreover, accurate identification and quantification, in the SAM EGA mode, of volatiles released from the heated sample, or generated by reactions occurring in the SAM pyrolysis oven, is also difficult for a few compounds due to evolution over similar temperature ranges and overlap of their MS signatures. Hence, the GC analyses, coupled with MS, enabled the separation and identification and quantification of most of the volatile compounds detected. These results can have been obtained through tests and calibration done with GC individual spare components and with the SAM testbed. This paper will present a view of the interpretation of the chromatograms obtained when analyzing the Rocknest and John Klein solid samples delivered to SAM, on sols 96 and 199 respectively, supported by laboratory calibrations.

  14. When questions arise, physician advisors are there with the answers.

    PubMed

    2016-06-01

    DCH Health System's seven part-time physician advisors perform a variety of tasks to help ensure that the hospitals are appropriately reimbursed and that patients move smoothly through the continuum of care. They work with care managers on patient status and also assist with documentation, writing medical necessity appeals, resource utilization, and patient throughput. They attend daily transition rounds with the nurses, care managers, and social workers, and participate in weekly long-stay rounds and capacity rounds in the ICU and acute cardiac care. The health system brought in experts to educate the physician advisors, sent them to educational symposia, and pays for membership in professional organizations. PMID:27323508

  15. 75 FR 11949 - Lincoln Investment Advisors Corporation and Lincoln Variable Insurance Products Trust; Notice of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-12

    ... insurance policy and variable annuity contract owners that are unitholders of any separate account for which... COMMISSION Lincoln Investment Advisors Corporation and Lincoln Variable Insurance Products Trust; Notice of... requirements. Applicants: Lincoln Investment Advisors Corporation (``Adviser'') and Lincoln Variable...

  16. 76 FR 7593 - President's Council of Advisors on Science and Technology; Notice of Meeting: Partially Closed...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-10

    ... two years of undergraduate science, technology, engineering, and mathematics education. Additional... TECHNOLOGY POLICY President's Council of Advisors on Science and Technology; Notice of Meeting: Partially Closed Meeting of the President's Council of Advisors on Science and Technology ACTION: Public...

  17. Beta test results for the CAA mini-SAM system

    SciTech Connect

    Johnson, R.C.; Monagle, M.

    1997-04-01

    The mission of the Chemical Analysis Automation (CAA) Program is to automate methods for chemical analysis of environmental samples. To accomplish this mission, the CAA team has developed automated laboratory systems based on a plug-and-work strategy for integrating components. Realizing that standardization is the key to implementing this strategy, CAA has developed, demonstrated, and encouraged commercialization of standards for laboratory automation. While the CAA mission is driven by the analyses in support of the extensive remediation programs of the Departments of Energy and Defense, it also impacts any industry that depends upon high volumes of repetitive chemical analysis. A Standard Analysis Method (SAM) is any collection of hardware and software used to automate part or all of a method. The method automated for the Mini-SAM testing is EPA Method 3550, which outlines semivolatiles extraction by sonication. The list of semivolatiles includes the polychlorinated biphenyl (PCB) analytes of interest. The basic building block of a SAM is the Standard Laboratory Module (SLM). For the Mini-SAM test an automated sonication SLM and an automated concentration SLM were configured to perform the extraction and concentration processes. The Mini-SAM differs from the Full-SAM in that a fully automated delivery of materials, samples, and extracts is not required. The intent of the Beta Test of the Mini-SAM was threefold. Firstly, the Mini-SAM Beta Test met a milestone mandated by the Department of Energy in the course of the program effort. Secondly, the CAA Program secured an independent assessment of the equipment and its capabilities from Assagai Analytical Laboratory. Lastly, the Program captured real-world sample data. The independent assessment, coupled with CAA observation of equipment performance, was used to determine strengths and weaknesses of the Mini-SAM and to compile possible modifications for CAA engineers to address.

  18. SAM 2 and SAGE data management and processing

    NASA Technical Reports Server (NTRS)

    Osborn, M. T.; Trepte, C. R.

    1987-01-01

    The data management and processing supplied by ST Systems Corporation (STX) for the Stratospheric Aerosol Measurement 2 (SAM 2) and Stratospheric Aerosol and Gas Experiment (SAGE) experiments for the years 1983 to 1986 are described. Included are discussions of data validation, documentation, and scientific analysis, as well as the archival schedule met by the operational reduction of SAM 2 and SAGE data. Work under this contract resulted in the archiving of the first seven years of SAM 2 data and all three years of SAGE data. A list of publications and presentations supported was also included.

  19. Recent Advances in Radical SAM Enzymology: New Structures and Mechanisms

    PubMed Central

    2015-01-01

    The radical S-adenosylmethionine (SAM) superfamily of enzymes catalyzes an amazingly diverse variety of reactions ranging from simple hydrogen abstraction to complicated multistep rearrangements and insertions. The reactions they catalyze are important for a broad range of biological functions, including cofactor and natural product biosynthesis, DNA repair, and tRNA modification. Generally conserved features of the radical SAM superfamily include a CX3CX2C motif that binds an [Fe4S4] cluster essential for the reductive cleavage of SAM. Here, we review recent advances in our understanding of the structure and mechanisms of these enzymes that, in some cases, have overturned widely accepted mechanisms. PMID:25009947

  20. SAM 2 and SAGE data management and processing

    NASA Astrophysics Data System (ADS)

    Osborn, M. T.; Trepte, C. R.

    1987-02-01

    The data management and processing supplied by ST Systems Corporation (STX) for the Stratospheric Aerosol Measurement 2 (SAM 2) and Stratospheric Aerosol and Gas Experiment (SAGE) experiments for the years 1983 to 1986 are described. Included are discussions of data validation, documentation, and scientific analysis, as well as the archival schedule met by the operational reduction of SAM 2 and SAGE data. Work under this contract resulted in the archiving of the first seven years of SAM 2 data and all three years of SAGE data. A list of publications and presentations supported was also included.

  1. Crystal structures of the SAM-III/S[subscript MK] riboswitch reveal the SAM-dependent translation inhibition mechanism

    SciTech Connect

    Lu, C.; Smith, A.M.; Fuchs, R.T.; Ding, F.; Rajashankar, K.; Henkin, T.M.; Ke, A.

    2010-01-07

    Three distinct classes of S-adenosyl-L-methionine (SAM)-responsive riboswitches have been identified that regulate bacterial gene expression at the levels of transcription attenuation or translation inhibition. The SMK box (SAM-III) translational riboswitch has been identified in the SAM synthetase gene in members of the Lactobacillales. Here we report the 2.2-{angstrom} crystal structure of the Enterococcus faecalis SMK box riboswitch. The Y-shaped riboswitch organizes its conserved nucleotides around a three-way junction for SAM recognition. The Shine-Dalgarno sequence, which is sequestered by base-pairing with the anti-Shine-Dalgarno sequence in response to SAM binding, also directly participates in SAM recognition. The riboswitch makes extensive interactions with the adenosine and sulfonium moieties of SAM but does not appear to recognize the tail of the methionine moiety. We captured a structural snapshot of the SMK box riboswitch sampling the near-cognate ligand S-adenosyl-L-homocysteine (SAH) in which SAH was found to adopt an alternative conformation and fails to make several key interactions.

  2. Vibrational Stark Effect of the Electric-Field Reporter 4-Mercaptobenzonitrile as a Tool for Investigating Electrostatics at Electrode/SAM/Solution Interfaces

    PubMed Central

    Schkolnik, Gal; Salewski, Johannes; Millo, Diego; Zebger, Ingo; Franzen, Stefan; Hildebrandt, Peter

    2012-01-01

    4-mercaptobenzonitrile (MBN) in self-assembled monolayers (SAMs) on Au and Ag electrodes was studied by surface enhanced infrared absorption and Raman spectroscopy, to correlate the nitrile stretching frequency with the local electric field exploiting the vibrational Stark effect (VSE). Using MBN SAMs in different metal/SAM interfaces, we sorted out the main factors controlling the nitrile stretching frequency, which comprise, in addition to external electric fields, the metal-MBN bond, the surface potential, and hydrogen bond interactions. On the basis of the linear relationships between the nitrile stretching and the electrode potential, an electrostatic description of the interfacial potential distribution is presented that allows for determining the electric field strengths on the SAM surface, as well as the effective potential of zero-charge of the SAM-coated metal. Comparing this latter quantity with calculated values derived from literature data, we note a very good agreement for Au/MBN but distinct deviations for Ag/MBN which may reflect either the approximations and simplifications of the model or the uncertainty in reported structural parameters for Ag/MBN. The present electrostatic model consistently explains the electric field strengths for MBN SAMs on Ag and Au as well as for thiophenol and mercaptohexanoic acid SAMs with MBN incorporated as a VSE reporter. PMID:22837705

  3. 17 CFR 4.14 - Exemption from registration as a commodity trading advisor.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... a commodity trading advisor. 4.14 Section 4.14 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION COMMODITY POOL OPERATORS AND COMMODITY TRADING ADVISORS General Provisions, Definitions and Exemptions § 4.14 Exemption from registration as a commodity trading advisor. This section...

  4. 17 CFR 4.27 - Additional reporting by advisors of certain large commodity pools.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... advisors of certain large commodity pools. 4.27 Section 4.27 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION COMMODITY POOL OPERATORS AND COMMODITY TRADING ADVISORS Commodity Pool Operators § 4.27 Additional reporting by advisors of certain large commodity pools. (a) General...

  5. 17 CFR 4.27 - Additional reporting by advisors of certain large commodity pools.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... advisors of certain large commodity pools. 4.27 Section 4.27 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION COMMODITY POOL OPERATORS AND COMMODITY TRADING ADVISORS Commodity Pool Operators § 4.27 Additional reporting by advisors of certain large commodity pools. (a) General...

  6. 17 CFR 4.27 - Additional reporting by advisors of commodity pools.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... advisors of commodity pools. 4.27 Section 4.27 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION COMMODITY POOL OPERATORS AND COMMODITY TRADING ADVISORS Commodity Pool Operators § 4.27 Additional reporting by advisors of commodity pools. Except as otherwise expressly provided in this section, CPOs...

  7. 76 FR 30323 - President's Board of Advisors on Historically Black Colleges and Universities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-25

    ... President's Board of Advisors on Historically Black Colleges and Universities AGENCY: U.S. Department of Education, President's Board of Advisors on Historically Black Colleges and Universities (Board). ACTION... President's Board of Advisors on Historically Black Colleges and Universities. The notice also describes...

  8. 76 FR 7550 - President's Board of Advisors on Historically Black Colleges and Universities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-10

    ... President's Board of Advisors on Historically Black Colleges and Universities AGENCY: U.S. Department of Education, President's Board of Advisors on Historically Black Colleges and Universities (Board). ACTION... President's Board of Advisors on Historically Black Colleges and Universities. The notice also describes...

  9. 78 FR 57135 - President's Board of Advisors on Historically Black Colleges and Universities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-17

    ... President's Board of Advisors on Historically Black Colleges and Universities AGENCY: U.S. Department of Education, President's Board of Advisors on Historically Black Colleges and Universities. ACTION: Notice of... President's Board of Advisors on Historically Black Colleges and Universities. The notice also describes...

  10. 76 FR 70779 - President's Council of Advisors on Science and Technology

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-15

    ... TECHNOLOGY POLICY President's Council of Advisors on Science and Technology Notice of Meeting: Open Regional Meeting of the President's Council of Advisors on Science and Technology, Working Group on Advanced... open regional meeting of the President's Council of Advisors on Science and Technology (PCAST),...

  11. 76 FR 70780 - President's Council of Advisors on Science and Technology

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-15

    ... TECHNOLOGY POLICY President's Council of Advisors on Science and Technology Notice of Meeting: Open Regional Meeting of the President's Council of Advisors on Science and Technology, Working Group on Advanced... open regional meeting of the President's Council of Advisors on Science and Technology (PCAST),...

  12. Future Farmers of America--A Profile of Chapters and Advisors.

    ERIC Educational Resources Information Center

    Gordon, Howard R. D.

    A study was conducted to collect information related to Future Farmers of America (FFA) advisors, chapters, and school characteristics. Specific objectives were to determine FFA activities influenced by the advisor's experience, and to identify factors perceived as contributing to the preparation of teachers for the role of advisor and to the…

  13. 78 FR 59013 - President's Council of Advisors on Science and Technology

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-25

    ... President's Council of Advisors on Science and Technology AGENCY: Department of Energy. ACTION: Notice of... call of the President's Council of Advisors on Science and Technology (PCAST), and describes the... Advisors on Science and Technology (PCAST) is an advisory group of the nation's leading scientists...

  14. 77 FR 74230 - PNC Capital Advisors, LLC, et al.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-13

    ... COMMISSION PNC Capital Advisors, LLC, et al.; Notice of Application December 6, 2012. AGENCY: Securities and... the Act to invest in certain financial instruments. Applicants: PNC Capital Advisors, LLC (``PNC Capital Advisors''), PNC Funds and PNC Advantage Funds (together, the ``Trusts''), and PNC...

  15. 77 FR 38277 - President's Council of Advisors on Science and Technology Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-27

    ... President's Council of Advisors on Science and Technology Meeting AGENCY: Department of Energy, DoE. ACTION... meeting of the President's Council of Advisors on Science and Technology (PCAST), and describes the... INFORMATION: The President's Council of Advisors on Science and Technology (PCAST) is an advisory group of...

  16. 75 FR 47317 - President's Council of Advisors on Science and Technology

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-05

    ... TECHNOLOGY POLICY President's Council of Advisors on Science and Technology Notice of Meeting: Partially Closed Meeting of the President's Council of Advisors on Science and Technology. ACTION: Public notice... President's Council of Advisors on Science and Technology (PCAST), and describes the functions of...

  17. 77 FR 51790 - President's Council of Advisors on Science and Technology (PCAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-27

    ... President's Council of Advisors on Science and Technology (PCAST) AGENCY: Department of Energy. ACTION... partially closed meeting of the President's Council of Advisors on Science and Technology (PCAST), and...: The President's Council of Advisors on Science and Technology (PCAST) is an advisory group of...

  18. 77 FR 10736 - President's Council of Advisors on Science and Technology (PCAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-23

    ... President's Council of Advisors on Science and Technology (PCAST) AGENCY: Department of Energy, DOE. ACTION... partially closed meeting of the President's Council of Advisors on Science and Technology (PCAST), and.... SUPPLEMENTARY INFORMATION: The President's Council of Advisors on Science and Technology (PCAST) is an...

  19. 75 FR 53279 - President's Board of Advisors on Historically Black Colleges and Universities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-31

    ... President's Board of Advisors on Historically Black Colleges and Universities AGENCY: U.S. Department of Education, President's Board of Advisors on Historically Black Colleges and Universities (Board). ACTION... President's Board of Advisors on Historically Black Colleges and Universities. The notice also describes...

  20. 76 FR 35054 - President's Council of Advisors on Science and Technology; Notice of Partially Closed Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-15

    ... TECHNOLOGY POLICY President's Council of Advisors on Science and Technology; Notice of Partially Closed... partially closed meeting of the President's Council of Advisors on Science and Technology (PCAST), and... Closed. Proposed Schedule and Agenda: The President's Council of Advisors on Science and...

  1. 77 FR 26273 - President's Council of Advisors on Science and Technology (PCAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-03

    ... President's Council of Advisors on Science and Technology (PCAST) AGENCY: Department of Energy. ACTION... partially closed meeting of the President's Council of Advisors on Science and Technology (PCAST), and... INFORMATION: The President's Council of Advisors on Science and Technology (PCAST) is an advisory group of...

  2. A Qualitative Inquiry into the Training and Development Provided to Community College Academic Advisors

    ERIC Educational Resources Information Center

    Mikluscak, George Steven, Jr.

    2010-01-01

    The qualitative study explored the training and development provided to Community College academic advisors who are members of the National Academic Advising Association (NACADA). The purpose was to investigate the factors academic advisors believe are crucial for the support of their roles as advisors. Professional, faculty, and self-identified…

  3. Curiosity Shakes, Bakes, and Tastes Mars with SAM

    NASA Video Gallery

    NASA's Curiosity rover analyzed its first solid sample of Mars with a variety of instruments, including the Sample Analysis at Mars (SAM) instrument suite. Developed at NASA's Goddard Space Flight ...

  4. Training Mentors as Educational Advisors for Adult Learners. Process Manual.

    ERIC Educational Resources Information Center

    Singer, Elizabeth W.

    Materials used by the director of a project designed to assist key personnel in businesses, industries, and agencies with the educational counseling of their employees are presented. The objectives of the project, "Training Mentors as Educational Advisors of Adult Learners," were to: (1) provide two training sessions in educational counseling…

  5. Risk Management in College Fraternities: Guidance from Two Faculty Advisors

    ERIC Educational Resources Information Center

    Rosenberg, Stuart; Mosca, Joseph

    2016-01-01

    Students who become members of fraternities and sororities avail themselves of significant opportunities that enrich their college life. Faculty advisors play an important role in assisting fraternities and sororities in shaping students' leadership, scholastic, and personal development. Given the risks such as alcohol use and hazing that continue…

  6. Database Advisor: A New Tool for K-12 Research Projects.

    ERIC Educational Resources Information Center

    Berteaux, Susan S.; Strong, Sandra S.

    The Database Advisor (DBA) is a tool designed to guide users to the most appropriate World Wide Web-based databases for their research. Developed in 1997 by the Science Libraries at the University of California, San Diego (UCSD), DBA is a Web-based front-end to bibliographic and full-text databases to which UCSD has remote access. DBA allows the…

  7. Attending the AAHPERD National Convention: A Guide for Faculty Advisors

    ERIC Educational Resources Information Center

    Darden, Gibson; Nelson, Sandra; Parsons, Roberta

    2005-01-01

    Student attendance at national conventions can be an important part of professional growth. Faculty advisors and administrators can increase the quality of future HPERD professionals by making greater use of this resource. Taking students to a convention may appear a daunting task. However, establishing a systematic process may help create a…

  8. Choosing between Hearts and Minds: Children's Understanding of Moral Advisors

    ERIC Educational Resources Information Center

    Danovitch, Judith H.; Keil, Frank C.

    2007-01-01

    Moral development research has often focused on the development of moral reasoning without considering children's understanding of moral advisors. We investigated how children construe sources of moral advice by examining the characteristics that children deem necessary for reasoning about moral or scientific problems. In two experiments, children…

  9. The Use of Images in Intelligent Advisor Systems.

    ERIC Educational Resources Information Center

    Boulet, Marie-Michele

    This paper describes the intelligent advisor system, named CODAMA, used in teaching a university-level systems analysis and design course. The paper discusses: (1) the use of CODAMA to assist students to transfer theoretical knowledge to the practical; (2) details of how CODAMA is applied in conjunction with a computer-aided software engineering…

  10. Increasing Advisor Effectiveness by Understanding Conflict and Conflict Resolution

    ERIC Educational Resources Information Center

    McClellan, Jeffrey

    2005-01-01

    On a daily basis, advisors encounter various types of interpersonal and intrapersonal conflict. Through this article, the reader will better understand conflict, its positive and negative impacts and the approaches of the actors experiencing conflict, and the means whereby conflicts arise, escalate, and come to resolution in advising situations.…

  11. Student Preferences for Academic Advisors as Transformational Leaders

    ERIC Educational Resources Information Center

    Drozd, Deborah Suzanne

    2010-01-01

    A quantitative study was conducted to determine the preferences of undergraduate students for academic advisors as transformational leaders. A questionnaire was developed and distributed to undergraduate students enrolled in leadership classes at a major land grant university to determine importance ratings based on the variables including…

  12. New Routes To Transfer. The Advisor. November 2001

    ERIC Educational Resources Information Center

    British Columbia Council on Admissions and Transfer, 2001

    2001-01-01

    The Advisor provides important information or "system alerts," in user-friendly format and language, on issues and changes affecting admissions and transfer. This document outlines new transfer possibilities in academic arts and science programs, and business diploma programs -- the two largest programs offered by the colleges, university colleges…

  13. Extension of sunset date for attorney advisor program. Final rule.

    PubMed

    2013-07-29

    We are extending for 2 years our rule authorizing attorney advisors to conduct certain prehearing procedures and to issue fully favorable decisions. The current rule will expire on August 9, 2013. In this final rule, we are extending the sunset date to August 7, 2015. We are making no other substantive changes.

  14. 77 FR 53941 - Emerging Global Advisors, LLC, et al.;

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-04

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION Emerging Global Advisors, LLC, et al.; Notice of Application August 28, 2012. AGENCY: Securities and Exchange Commission (``Commission''). ACTION: Notice of an application for an order under...

  15. Making a Sales Advisor: The Limits of Training "Instrumental Empathy"

    ERIC Educational Resources Information Center

    Kakavelakis, Konstantinos; Felstead, Alan; Fuller, Alison; Jewson, Nick; Unwin, Lorna

    2008-01-01

    The use of participant observation is relatively rare in qualitative studies of vocational education and training. However, such an approach provides a detailed picture of training content and how what is taught contributes to or impedes learning. Based on participant observation, this paper examines the training of sales advisors in a large chain…

  16. LIA: An Intelligent Advisor for E-Learning

    ERIC Educational Resources Information Center

    Capuano, Nicola; Gaeta, Matteo; Marengo, Agostino; Miranda, Sergio; Orciuoli, Francesco; Ritrovato, Pierluigi

    2009-01-01

    Intelligent e-learning systems have revolutionized online education by providing individualized and personalized instruction for each learner. Nevertheless, until now very few systems were able to leave academic laboratories and be integrated into real commercial products. One of these few exceptions is the Learning Intelligent Advisor (LIA)…

  17. In vivo CH3(CH2)11SAu SAM electrodes in the beating heart: in situ analytical studies relevant to pacemakers and interstitial biosensors.

    PubMed

    Chou, Howard A; Zavitz, Daniel H; Ovadia, Marc

    2003-01-01

    To study in vivo modification of the SAM equivalent circuit when a highly ordered SAM is used as a bioelectrode, dodecanethiolate SAM-Au intramuscular electrodes were studied in living rat heart in a challenging in situ perfused rat model by impedance spectroscopy, cyclic voltammetry, and neutron activation analysis (NAA). The SAM layer experienced disintegration in vivo biological system, as NAA detected the presence of Au atoms that had leached into the surrounding living tissue. Therefore, the underlying Au surface became exposed during biological implant. Study by impedance spectroscopy, however, revealed perfect capacitive behavior for the SAM, similar to in vitro behavior. Electrodes showed a pure capacitive Nyquist plot with 86.1-89.4 degrees near-vertical line segments as the equivalent circuit locus, as for a parallel plate capacitor. Impedance magnitude varied linearly with 1/omega excluding diffusionally limited ionic charge transport. There was no diffusional conductive element Z(W infinity ) or spatially confined Warburg impedance Z(D). The effect of in vivo exposure of a highly ordered SAM is a 'sealing over' effect of new defects by the binding of proteinaceous or lipid species in the biological milieu, a fact of significance for SAM electrodes used either as pacemaker electrodes or as a platform for in vivo biosensors.

  18. Auxiliary iron-sulfur cofactors in radical SAM enzymes.

    PubMed

    Lanz, Nicholas D; Booker, Squire J

    2015-06-01

    A vast number of enzymes are now known to belong to a superfamily known as radical SAM, which all contain a [4Fe-4S] cluster ligated by three cysteine residues. The remaining, unligated, iron ion of the cluster binds in contact with the α-amino and α-carboxylate groups of S-adenosyl-l-methionine (SAM). This binding mode facilitates inner-sphere electron transfer from the reduced form of the cluster into the sulfur atom of SAM, resulting in a reductive cleavage of SAM to methionine and a 5'-deoxyadenosyl radical. The 5'-deoxyadenosyl radical then abstracts a target substrate hydrogen atom, initiating a wide variety of radical-based transformations. A subset of radical SAM enzymes contains one or more additional iron-sulfur clusters that are required for the reactions they catalyze. However, outside of a subset of sulfur insertion reactions, very little is known about the roles of these additional clusters. This review will highlight the most recent advances in the identification and characterization of radical SAM enzymes that harbor auxiliary iron-sulfur clusters. This article is part of a Special Issue entitled: Fe/S proteins: Analysis, structure, function, biogenesis and diseases.

  19. Teacher-Advisors in a Ninth Grade Guidance Program. Project Description, Administrator's Guide, Teacher-Advisor Guidance Manual.

    ERIC Educational Resources Information Center

    Dudley, Robert T.; McDermott, William P.

    The project aims to provide adequate guidance services to all ninth grade students upon their entry into secondary school, and throughout the crucial first year, to assist them with their adjustment. Through the efforts of Teacher-Advisors who fill the roll of counselors, students are encouraged to develop good study and attendance habits and to…

  20. 26 CFR 301.6112-1 - Material advisors of reportable transactions must keep lists of advisees, etc.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... whom the advisor acted as a material advisor with respect to the reportable transaction. However, a... by the material advisor; (D) The amount invested in each reportable transaction by each person... transaction; and (F) The name of each other material advisor to the transaction, if known by the...

  1. SAMS Acceleration Measurements on Mir from June to November 1995

    NASA Technical Reports Server (NTRS)

    DeLombard, Richard; Hrovat, Ken; Moskowitz, Milton; McPherson, Kevin

    1996-01-01

    The NASA Microgravity Science and Applications Division (MSAD) sponsors science experiments on a variety of microgravity carriers, including sounding rockets, drop towers, parabolic aircraft, and Orbiter missions. The MSAD sponsors the Space Acceleration Measurement System (SAMS) to support microgravity science experiments with acceleration measurements to characterize the microgravity environment to which the experiments were exposed. The Principal Investigator Microgravity Services project at the NASA Lewis Research Center supports principal investigators of microgravity experiments as they evaluate the effects of varying acceleration levels on their experiments. In 1993, a cooperative effort was started between the United States and Russia involving science utilization of the Russian Mir space station by scientists from the United States and Russia. MSAD is currently sponsoring science experiments participating in the Shuttle-Mir Science Program in cooperation with the Russians on the Mir space station. Included in the complement of MSAD experiments and equipment is a SAMS unit In a manner similar to Orbiter mission support, the SAMS unit supports science experiments from the U.S. and Russia by measuring the microgravity environment during experiment operations. The initial SAMS supported experiment was a Protein Crystal Growth (PCG) experiment from June to November 1995. SAMS data were obtained during the PCG operations on Mir in accordance with the PCG Principal Investigator's requirements. This report presents an overview of the SAMS data recorded to support this PCG experiment. The report contains plots of the SAMS 100 Hz sensor head data as an overview of the microgravity environment, including the STS-74 Shuttle-Mir docking.

  2. Photovoltaic performance models: an evaluation with actual field data

    NASA Astrophysics Data System (ADS)

    TamizhMani, Govindasamy; Ishioye, John-Paul; Voropayev, Arseniy; Kang, Yi

    2008-08-01

    Prediction of energy production is crucial to the design and installation of the building integrated photovoltaic systems. This prediction should be attainable based on the commonly available parameters such as system size, orientation and tilt angle. Several commercially available as well as free downloadable software tools exist to predict energy production. Six software models have been evaluated in this study and they are: PV Watts, PVsyst, MAUI, Clean Power Estimator, Solar Advisor Model (SAM) and RETScreen. This evaluation has been done by comparing the monthly, seasonaly and annually predicted data with the actual, field data obtained over a year period on a large number of residential PV systems ranging between 2 and 3 kWdc. All the systems are located in Arizona, within the Phoenix metropolitan area which lies at latitude 33° North, and longitude 112 West, and are all connected to the electrical grid.

  3. CONSORT: Sam68 Is Directly Regulated by MiR-204 and Promotes the Self-Renewal Potential of Breast Cancer Cells by Activating the Wnt/Beta-Catenin Signaling Pathway

    PubMed Central

    Wang, Lan; Tian, Han; Yuan, Jie; Wu, Hongmei; Wu, Jueheng; Zhu, Xun

    2015-01-01

    Abstract Breast cancer stem cells (BCSCs) are considered to be responsible for recurrence in breast cancer. The 68 kDa Src-associated protein in mitosis (Sam68) has been linked to the development and progression of breast cancer; however, the posttranscriptional regulation and role of Sam68 in BCSC self-renewal remain unclear. Sam68 was ectopically overexpressed or knocked down using a siRNA; the self-renewal potential of breast cancer cell lines was assessed using flow cytometry, in vitro mammosphere culture and a xenograft model in NOD/SCID mice. Activation of beta-catenin was assessed by immunohistochemical staining, Western blotting, and luciferase reporter gene assays. The ArrayExpress dataset GSE45666 was used to identify conserved microRNAs downregulated in breast cancer; real-time PCR, Western blotting, luciferase reporter assay, and xenografted tumor model were used to confirm miR-204 regulated Sam68. We found that endogenous Sam68 expression correlated positively with the self-renewal potential of breast cancer cell lines. Overexpression of Sam68 promoted, whereas knockdown reduced, breast cancer cell self-renewal potential in vitro and tumorigenicity in vivo. The Wnt/beta-catenin pathway was identified as a functional mediator of Sam68-induced self-renewal in SKBR-3 and MCF-7 cells. Furthermore, miR-204 was found to be frequently downregulated in human breast cancer and confirmed to directly target Sam68; miR-204 inhibited the self-renewal of breast cancer cell lines by targeting and suppressing Sam68. Our study reveals that Sam68 is regulated by miR-204 and may play an important role in the self-renewal of BCSCs via activating the Wnt/beta-catenin pathway. Sam68 may represent a novel therapeutic target for breast cancer. PMID:26656364

  4. 76 FR 80733 - Extension of Temporary Registration of Municipal Advisors

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-27

    ...The Securities and Exchange Commission (``Commission'') is amending interim final temporary Rule 15Ba2-6T, which provides for the temporary registration of municipal advisors under the Securities Exchange Act of 1934 (``Exchange Act''), as amended by the Dodd-Frank Wall Street Reform and Consumer Protection Act (``Dodd-Frank Act''), to extend the date on which Rule 15Ba2-6T will sunset from......

  5. SAM-T08, HMM-based protein structure prediction

    PubMed Central

    Karplus, Kevin

    2009-01-01

    The SAM-T08 web server is a protein structure prediction server that provides several useful intermediate results in addition to the final predicted 3D structure: three multiple sequence alignments of putative homologs using different iterated search procedures, prediction of local structure features including various backbone and burial properties, calibrated E-values for the significance of template searches of PDB and residue–residue contact predictions. The server has been validated as part of the CASP8 assessment of structure prediction as having good performance across all classes of predictions. The SAM-T08 server is available at http://compbio.soe.ucsc.edu/SAM_T08/T08-query.html PMID:19483096

  6. The methylthiolation reaction mediated by the Radical-SAM enzymes

    PubMed Central

    Atta, Mohamed; Arragain, Simon; Fontecave, Marc; Mulliez, Etienne; Hunt, John F.; Luff, Jon D.; Forouhar, Farhad

    2014-01-01

    Over the past ten years, considerable progress has been made in our understanding of the mechanistic enzymology of the Radical-SAM enzymes. It is now clear that these enzymes appear to be involved in a remarkably wide range of chemically challenging reactions. This review article highlights mechanistic and structural aspects of the methylthiotransferases (MTTases) sub-class of the Radical-SAM enzymes. The mechanism of methylthio insertion, now observed to be performed by three different enzymes is an exciting unsolved problem. PMID:22178611

  7. VAS: A Vision Advisor System combining agents and object-oriented databases

    NASA Technical Reports Server (NTRS)

    Eilbert, James L.; Lim, William; Mendelsohn, Jay; Braun, Ron; Yearwood, Michael

    1994-01-01

    A model-based approach to identifying and finding the orientation of non-overlapping parts on a tray has been developed. The part models contain both exact and fuzzy descriptions of part features, and are stored in an object-oriented database. Full identification of the parts involves several interacting tasks each of which is handled by a distinct agent. Using fuzzy information stored in the model allowed part features that were essentially at the noise level to be extracted and used for identification. This was done by focusing attention on the portion of the part where the feature must be found if the current hypothesis of the part ID is correct. In going from one set of parts to another the only thing that needs to be changed is the database of part models. This work is part of an effort in developing a Vision Advisor System (VAS) that combines agents and objected-oriented databases.

  8. Atomic-level insights into metabolite recognition and specificity of the SAM-II riboswitch

    PubMed Central

    Doshi, Urmi; Kelley, Jennifer M.; Hamelberg, Donald

    2012-01-01

    Although S-adenosylhomocysteine (SAH), a metabolic by-product of S-adenosylmethionine (SAM), differs from SAM only by a single methyl group and an overall positive charge, SAH binds the SAM-II riboswitch with more than 1000-fold less affinity than SAM. Using atomistic molecular dynamics simulations, we investigated the molecular basis of such high selectivity in ligand recognition by SAM-II riboswitch. The biosynthesis of SAM exclusively generates the (S,S) stereoisomer, and (S,S)-SAM can spontaneously convert to the (R,S) form. We, therefore, also examined the effects of (R,S)-SAM binding to SAM-II and its potential biological function. We find that the unfavorable loss in entropy in SAM-II binding is greater for (S,S)- and (R,S)-SAM than SAH, which is compensated by stabilizing electrostatic interactions with the riboswitch. The positively charged sulfonium moiety on SAM acts as the crucial anchor point responsible for the formation of key ionic interactions as it fits favorably in the negatively charged binding pocket. In contrast, SAH, with its lone pair of electrons on the sulfur, experiences repulsion in the binding pocket of SAM-II and is enthalpically destabilized. In the presence of SAH, similar to the unbound riboswitch, the pseudoknot structure of SAM-II is not completely formed, thus exposing the Shine-Dalgarno sequence. Unlike SAM, this may further facilitate ribosomal assembly and translation initiation. Our analysis of the conformational ensemble sampled by SAM-II in the absence of ligands and when bound to SAM or SAH reveals that ligand binding follows a combination of conformational selection and induced-fit mechanisms. PMID:22194311

  9. Sam68 functions as a transcriptional coactivator of the p53 tumor suppressor

    PubMed Central

    Li, Naomi; Richard, Stéphane

    2016-01-01

    Sam68 is a known sequence-specific RNA binding protein that regulates alternative splicing events during the cell cycle and apoptosis. Sam68 has also been shown to influence transcription, but the molecular mechanism remains undefined. Herein we identify Sam68 as a transcriptional coactivator of the p53 tumor suppressor in response to DNA damage. Using CRISPR/Cas9 generated isogenic HCT116 Sam68−/− cell lines wild type or deficient for p53, we show that Sam68 is required for the efficient transactivation of p53 target genes. Consistently, Sam68 depletion caused defects in DNA damage-induced cell cycle arrest and apoptosis mediated by p53. Mechanistically, we demonstrate that Sam68 physically interacted with p53 in an RNA-dependent manner, and that this interaction was essential for the coactivator function of Sam68. Furthermore, we show that both Sam68 and p53 were recruited to promoters of p53-responsive genes, suggesting interdependence. Finally, Sam68 acted in concert with the p53 long noncoding RNA (lncRNA) target PR-lncRNA-1 for p53 recruitment, implicating a positive-feedback mechanism in which lncRNAs induced by the Sam68/p53 complex can enhance p53 transcriptional activity. These findings define a hitherto novel mechanism of action for Sam68 in governing p53 transcriptional activation, and represent the first report of Sam68 in the regulation of tumor suppressor activities. PMID:27365047

  10. Skill Acquisition Measures (SAM). Elementary Mathematics Level IV. Teacher's Manual.

    ERIC Educational Resources Information Center

    Lancaster City School District, PA.

    The Skill Acquisition Measures (SAM) were designed by the Lancaster (Pennsylvania) School District as criterion referenced tests for mathematics. This manual consists of copies of the student test forms for level 4, with additional information for the teacher's use. Each of the test items is presented with the correct answers and the criteria for…

  11. 78 FR 47695 - Sam Rayburn Dam Power Rate

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-06

    ... September 30, 2013 (77 FR 67813, November. 14, 2012). The Administrator, Southwestern Power Administration (Southwestern), has prepared Current and Revised 2013 Power Repayment Studies which show the need for an... Southwestern Power Administration Sam Rayburn Dam Power Rate AGENCY: Southwestern Power Administration,...

  12. 77 FR 67813 - Sam Rayburn Dam Project Power Rate

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-14

    ... Rayburn power rate extension were announced by a Federal Register (77 FR 50493) notice published on August... published notice in the Federal Register, (77 FR 50493), of the proposed rate extension for the Rayburn... Southwestern Power Administration Sam Rayburn Dam Project Power Rate AGENCY: Southwestern Power...

  13. Social Activity Method (SAM): A Fractal Language for Mathematics

    ERIC Educational Resources Information Center

    Dowling, Paul

    2013-01-01

    In this paper I shall present and develop my organisational language, "social activity method" (SAM), and illustrate some of its applications. I shall introduce a new scheme for "modes of recontextualisation" that enables the analysis of the ways in which one activity--which might be school mathematics or social research or any…

  14. SAMS Acceleration Measurement on Mir From March to September 1996

    NASA Technical Reports Server (NTRS)

    Moskowitz, Milton E.; Hrovat, Ken; Truong, Duc; Reckart, Timothy

    1997-01-01

    During NASA Increment 2 (March to September 1996), over 15 gigabytes of acceleration data were collected by the Space Acceleration Measurement System (SAMS) onboard the Russian Space Station, Mir. The data were recorded on 55 optical disks and were returned to Earth on STS-79. During this time, SAMS data were collected in the Kristall and Kvant modules, and in the Priroda module to support the following experiments: the Queen's University Experiments in Liquid Diffusion (QUELD), the Technological Evaluation of the MIM (TEM), the Forced Flow Flame Spreading Test (FFFT), and Candle Flames in Microgravity (CFM). This report points out some of the salient features of the microgravity environment to which these experiments were exposed. Also documented are mission events of interest such as the docked phase of STS-76 operations, an extravehicular activity (EVA) to install and deploy solar panels on the Kvant module, a Progress engine burn to raise Mir's altitude, and an on-orbit SAMS calibration procedure. Also included are a description of the Mir module orientations, and the panel notations within the modules. This report presents an overview of the SAMS acceleration measurements recorded by 10 Hz and 100 Hz sensor heads. Variations in the acceleration environment caused by unique activities such as crew exercise and life-support fans are presented. The analyses included herein complement those presented in previous mission summary reports published by the Principal Investigator Microgravity Services (PIMS) group.

  15. Role of Sam68 in post-transcriptional gene regulation.

    PubMed

    Sánchez-Jiménez, Flora; Sánchez-Margalet, Víctor

    2013-11-28

    The STAR family of proteins links signaling pathways to various aspects of post-transcriptional regulation and processing of RNAs. Sam68 belongs to this class of heteronuclear ribonucleoprotein particle K (hnRNP K) homology (KH) single domain-containing family of RNA-binding proteins that also contains some domains predicted to bind critical components in signal transduction pathways. In response to phosphorylation and other post-transcriptional modifications, Sam68 has been shown to have the ability to link signal transduction pathways to downstream effects regulating RNA metabolism, including transcription, alternative splicing or RNA transport. In addition to its function as a docking protein in some signaling pathways, this prototypic STAR protein has been identified to have a nuclear localization and to take part in the formation of both nuclear and cytosolic multi-molecular complexes such as Sam68 nuclear bodies and stress granules. Coupling with other proteins and RNA targets, Sam68 may play a role in the regulation of differential expression and mRNA processing and translation according to internal and external signals, thus mediating important physiological functions, such as cell death, proliferation or cell differentiation.

  16. Role of Sam68 in Post-Transcriptional Gene Regulation

    PubMed Central

    Sánchez-Jiménez, Flora; Sánchez-Margalet, Víctor

    2013-01-01

    The STAR family of proteins links signaling pathways to various aspects of post-transcriptional regulation and processing of RNAs. Sam68 belongs to this class of heteronuclear ribonucleoprotein particle K (hnRNP K) homology (KH) single domain-containing family of RNA-binding proteins that also contains some domains predicted to bind critical components in signal transduction pathways. In response to phosphorylation and other post-transcriptional modifications, Sam68 has been shown to have the ability to link signal transduction pathways to downstream effects regulating RNA metabolism, including transcription, alternative splicing or RNA transport. In addition to its function as a docking protein in some signaling pathways, this prototypic STAR protein has been identified to have a nuclear localization and to take part in the formation of both nuclear and cytosolic multi-molecular complexes such as Sam68 nuclear bodies and stress granules. Coupling with other proteins and RNA targets, Sam68 may play a role in the regulation of differential expression and mRNA processing and translation according to internal and external signals, thus mediating important physiological functions, such as cell death, proliferation or cell differentiation. PMID:24287914

  17. Information System through ANIS at CeSAM

    NASA Astrophysics Data System (ADS)

    Moreau, C.; Agneray, F.; Gimenez, S.

    2015-09-01

    ANIS (AstroNomical Information System) is a web generic tool developed at CeSAM to facilitate and standardize the implementation of astronomical data of various kinds through private and/or public dedicated Information Systems. The architecture of ANIS is composed of a database server which contains the project data, a web user interface template which provides high level services (search, extract and display imaging and spectroscopic data using a combination of criteria, an object list, a sql query module or a cone search interfaces), a framework composed of several packages, and a metadata database managed by a web administration entity. The process to implement a new ANIS instance at CeSAM is easy and fast : the scientific project has to submit data or a data secure access, the CeSAM team installs the new instance (web interface template and the metadata database), and the project administrator can configure the instance with the web ANIS-administration entity. Currently, the CeSAM offers through ANIS a web access to VO compliant Information Systems for different projects (HeDaM, HST-COSMOS, CFHTLS-ZPhots, ExoDAT,...).

  18. EFFECTS OF POLYCYCLIC AROMATIC HYDROCARBON OF SAM-COATED ELECTRODES USING FERRYICYANIDE AS THE REDOX INDICATOR

    EPA Science Inventory

    Electrochemical responses on self-assembled monolayer (SAM)-coated polycrystalline gold electrodes were investigated using cyclic voltammetry and square wave voltammetry with a three electrode system. Experimental results show potential in the application of pyrene-imprinted SAM...

  19. Improving methionine and ATP availability by MET6 and SAM2 co-expression combined with sodium citrate feeding enhanced SAM accumulation in Saccharomyces cerevisiae.

    PubMed

    Chen, Hailong; Wang, Zhou; Wang, Zhilai; Dou, Jie; Zhou, Changlin

    2016-04-01

    S-adenosyl-L-methionine (SAM), biosynthesized from methionine and ATP, exhibited diverse pharmaceutical applications. To enhance SAM accumulation in S. cerevisiae CGMCC 2842 (wild type), improvement of methionine and ATP availability through MET6 and SAM2 co-expression combined with sodium citrate feeding was investigated here. Feeding 6 g/L methionine at 12 h into medium was found to increase SAM accumulation by 38 % in wild type strain. Based on this result, MET6, encoding methionine synthase, was overexpressed, which caused a 59 % increase of SAM. To redirect intracellular methionine into SAM, MET6 and SAM2 (encoding methionine adenosyltransferase) were co-expressed to obtain the recombinant strain YGSPM in which the SAM accumulation was 2.34-fold of wild type strain. The data obtained showed that co-expression of MET6 and SAM2 improved intracellular methionine availability and redirected the methionine to SAM biosynthesis. To elevate intracellular ATP levels, 6 g/L sodium citrate, used as an auxiliary energy substrate, was fed into the batch fermentation medium, and an additional 19 % increase of SAM was observed after sodium citrate addition. Meanwhile, it was found that addition of sodium citrate improved the isocitrate dehydrogenase activity which was associated with the intracellular ATP levels. The results demonstrated that addition of sodium citrate improved intracellular ATP levels which promoted conversion of methionine into SAM. This study presented a feasible approach with considerable potential for developing highly SAM-productive strains based on improving methionine and ATP availability.

  20. Spatio-angularly multiplexed (SAM) holographic storage in photorefractive crystals

    NASA Astrophysics Data System (ADS)

    Tao, Shiquan

    In this thesis a novel multiplexing scheme for dense holographic storage in photorefractive crystals, Spatio-Angular Multiplexing (or SAM), is described in detail. In SAM Fourier transform holograms are formed in spatially overlapping regions of a crystal and are distinguished from one another by using variously angled reference beams. SAM takes advantage of both the high storage density possible using angularly multiplexed volume holograms and also the low crosstalk possible using spatially multiplexed Fourier transform holograms. Compared to pure spatial multiplexing, SAM increases the storage capacity by fully utilising the volume of the storage medium. On the other hand, SAM reduces the number of holograms overlapping any one hologram in a given volume, and so increases the diffraction efficiency achievable as compared to pure angular multiplexing. SAM offers the possibility of incorporating the recorded crystal into a content addressable memory (CAM) system for parallel access of all stored patterns. In order to obtain the maximum diffraction efficiency and signal to noise ratio, the hologram must be replayed by a readout beam incident at the correct angle of readout beam. The optimum angle may be shifted away from the angle used in recording by a ''Bragg-shift", caused (under certain conditions) by phase coupling between the two writing beams during recording. Although this Bragg shift is small, a large diffraction efficiency enhancement is obtained when the grating is read out at the optimum angle. We have calculated the Bragg shift, using a numerical calculation based on an earlier theory, and have obtained good agreement with experiment. Using the novel SAM scheme, we have succeeded in storing 756 high resolution binary patterns in an Fe:LiNbO3 crystal of volume 1cm3, with an average diffraction efficiency of 0.5%. This large database is designed for practical use in a novel associative memory system, called a high order feedback neural network (HOFNET

  1. Characterization of sams genes of Amoeba proteus and the endosymbiotic X-bacteria.

    PubMed

    Jeon, Taeck J; Jeon, Kwang W

    2003-01-01

    As a result of harboring obligatory bacterial endosymbionts, the xD strain of Amoeba proteus no longer produces its own S-adenosylmethionine synthetase (SAMS). When symbiont-free D amoebae are infected with symbionts (X-bacteria), the amount of amoeba SAMS decreases to a negligible level within four weeks, but about 47% of the SAMS activity, which apparently comes from another source, is still detected. Complete nucleotide sequences of sams genes of D and xD amoebae are presented and show that there are no differences between the two. Long-established xD amoebae contain an intact sams gene and thus the loss of xD amoeba's SAMS is not due to the loss of the gene itself. The open reading frame of the amoeba's sams gene has 1,281 nucleotides, encoding SAMS of 426 amino acids with a mass of 48 kDa and pI of 6.5. The amino acid sequence of amoeba SAMS is longer than the SAMS of other organisms by having an extra internal stretch of 28 amino acids. The 5'-flanking region of amoeba sams contains consensus-binding sites for several transcription factors that are related to the regulation of sams genes in E. coli and yeast. The complete nucleotide sequence of the symbiont's sams gene is also presented. The open reading frame of X-bacteria sams is 1,146 nucleotides long, encoding SAMS of 381 amino acids with a mass of 41 kDa and pI of 6.0. The X-bacteria SAMS has 45% sequence identity with that of A. proteus.

  2. Selective Plasma Deposition of Fluorocarbon Films on SAMs

    NASA Technical Reports Server (NTRS)

    Crain, Mark M., III; Walsh, Kevin M.; Cohn, Robert W.

    2006-01-01

    A dry plasma process has been demonstrated to be useful for the selective modification of self-assembled monolayers (SAMs) of alkanethiolates. These SAMs are used, during the fabrication of semiconductor electronic devices, as etch masks on gold layers that are destined to be patterned and incorporated into the devices. The selective modification involves the formation of fluorocarbon films that render the SAMs more effective in protecting the masked areas of the gold against etching by a potassium iodide (KI) solution. This modification can be utilized, not only in the fabrication of single electronic devices but also in the fabrication of integrated circuits, microelectromechanical systems, and circuit boards. In the steps that precede the dry plasma process, a silicon mold in the desired pattern is fabricated by standard photolithographic techniques. A stamp is then made by casting polydimethylsiloxane (commonly known as silicone rubber) in the mold. The stamp is coated with an alkanethiol solution, then the stamp is pressed on the gold layer of a device to be fabricated in order to deposit the alkanethiol to form an alkanethiolate SAM in the desired pattern (see figure). Next, the workpiece is exposed to a radio-frequency plasma generated from a mixture of CF4 and H2 gases. After this plasma treatment, the SAM is found to be modified, while the exposed areas of gold remain unchanged. This dry plasma process offers the potential for forming masks superior to those formed in a prior wet etching process. Among the advantages over the wet etching process are greater selectivity, fewer pin holes in the masks, and less nonuniformity of the masks. The fluorocarbon films formed in this way may also be useful as intermediate layers for subsequent fabrication steps and as dielectric layers to be incorporated into finished products.

  3. Mentors, advisors and supervisors: their role in teaching responsible research conduct.

    PubMed

    Bird, S J

    2001-07-01

    Although the terms mentor and thesis advisor (or research supervisor) are often used interchangeably, the responsibilities associated with these roles are distinct, even when they overlap. Neither are role models necessarily mentors, though mentors are role models: good examples are necessary but not sufficient. Mentorship is both a personal and a professional relationship. It has the potential for raising a number of ethical concerns, including issues of accuracy and reliability of the information conveyed, access, stereotyping and tracking of advises, and the abuse of power. Nevertheless, mentors can be critically important for professional success and are one of a number of elements that affect the responsible conduct of research. In addition, the community as a whole has a responsibility to mentor junior members. PMID:11697001

  4. Standards Advisor-Advanced Information Technology for Advanced Information Delivery

    NASA Technical Reports Server (NTRS)

    Hawker, J. Scott

    2003-01-01

    Developers of space systems must deal with an increasing amount of information in responding to extensive requirements and standards from numerous sources. Accessing these requirements and standards, understanding them, comparing them, negotiating them and responding to them is often an overwhelming task. There are resources to aid the space systems developer, such as lessons learned and best practices. Again, though, accessing, understanding, and using this information is often more difficult than helpful. This results in space systems that: 1. Do not meet all their requirements. 2. Do not incorporate prior engineering experience. 3. Cost more to develop. 4. Take longer to develop. The NASA Technical Standards Program (NTSP) web site at http://standards.nasa.gov has made significant improvements in making standards, lessons learned, and related material available to space systems developers agency-wide. The Standards Advisor was conceived to take the next steps beyond the current product, continuing to apply evolving information technology that continues to improve information delivery to space systems developers. This report describes the features of the Standards Advisor and suggests a technical approach to its development.

  5. 77 FR 4289 - President's Board of Advisors on Historically Black Colleges and Universities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-27

    ..., University Student Center, 1700 E. Cold Spring Lane, Baltimore, Maryland 21251, (443) 885-4369. FOR FURTHER... President's Board of Advisors on Historically Black Colleges and Universities AGENCY: U.S. Department of Education, President's Board of Advisors on Historically Black Colleges and Universities (Board)....

  6. 78 FR 14297 - President's Board of Advisors on Historically Black Colleges and Universities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-05

    ... President's Board of Advisors on Historically Black Colleges and Universities AGENCY: U.S. Department of... Black Colleges and Universities scheduled for March 6, 2013, and announced in the Federal Register on... Advisors on Historically Black Colleges and Universities, U.S. Department of Education, 400 Maryland...

  7. 78 FR 12302 - President's Council of Advisors on Science and Technology (PCAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-22

    ... from speakers who will provide information on the National Math and Science Initiative, graduate... President's Council of Advisors on Science and Technology (PCAST) AGENCY: Office of Science, Department of... summary agenda for a partially closed meeting of the President's Council of Advisors on Science...

  8. "What Haven't I Learned?" Learning Resulting from the Resident Advisor Role

    ERIC Educational Resources Information Center

    Benjamin, Mimi; Davis, Laura

    2016-01-01

    Employment can enhance learning for many college students, and on-campus jobs provide unique opportunities for this experience. The resident advisor position is one on-campus position offering the potential for learning experiences. This qualitative study highlights learning noted by 78 resident advisors at a large research institution during a…

  9. Gay-Straight Alliance Advisors: Negotiating Multiple Ecological Systems when Advocating for LGBTQ Youth

    ERIC Educational Resources Information Center

    Watson, Laurel B.; Varjas, Kris; Meyers, Joel; Graybill, Emily C.

    2010-01-01

    This qualitative study investigated the barriers and facilitators that advisors of gay-straight alliances encounter when advocating for LGBTQ (lesbian, gay, bisexual, transgender, and queer) youth within schools. Twenty-two advisors were interviewed, and data revealed that multiple ecological systems (e.g., sociocultural, school, and individual…

  10. 76 FR 70781 - President's Council of Advisors on Science and Technology

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-15

    ... fertile environment for innovation and make investments to ensure that new technologies and design... TECHNOLOGY POLICY President's Council of Advisors on Science and Technology Notice of Meeting: Open Regional Meeting of the President's Council of Advisors on Science and Technology, Working Group on...

  11. Publication of a thesis: the relationship between graduate student and thesis advisor.

    PubMed

    Giefer, C

    1996-01-01

    Identifying the roles the graduate student and the thesis advisor will undertake when preparing the thesis helps when the thesis is published. Such issues as bylines and copyrights should be agreed upon before embarking on the thesis work. The author, an Associate Professor in the nursing department at a state university, clarifies the roles of the graduate student and thesis advisor.

  12. Perceptions of Freedom and Commitment as Sources of Self-Efficacy among Pedagogical Advisors

    ERIC Educational Resources Information Center

    Kass, Efrat; Rajuan, Maureen

    2012-01-01

    The perceptions of the role of the pedagogical advisor are multiple and diverse. This amorphous situation places importance on the sense of self-efficacy among those filling this role. Examined in this study was what pedagogical advisors perceive as factors affecting their professional self-efficacy through in-depth interviews of 10 experienced…

  13. Students Speak Out: Preliminary Qualitative Findings of a Mentor/Advisor Project.

    ERIC Educational Resources Information Center

    Welkowitz, Julie A.; Broer, Stephen M.; Topper, Karen; Thomas, Cassandra; Backus, Linda; Hamilton, Ruth

    This report explores the impact, through themes that emerged in interviews with 59 participating adolescents, of the Mentor/Advisor Program on students with or at risk of emotional disturbance. The Mentor/Advisor Program is implemented as part of the existing regular education high school curriculum and is designed to promote resiliency and school…

  14. Student Organization Advisor Motives to Volunteer at Four-Year Institutions of Higher Education

    ERIC Educational Resources Information Center

    Meyer, Robert A.; Kroth, Michael

    2010-01-01

    Many higher education institutions require student organization advisors to be employees. Such requirements can be interpreted as barriers to students and can limit the number and types of groups found on college campuses. Using quantitative analysis, this study investigates the motivation of student organization advisors at six public…

  15. 77 FR 18798 - President's Council of Advisors on Science and Technology (PCAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-28

    ... President's Council of Advisors on Science and Technology (PCAST) AGENCY: Department of Energy, Office of... agenda for an open conference call of the President's Council of Advisors on Science and Technology... President to augment the science and technology advice available to him from inside the White House and...

  16. 78 FR 46388 - NGAM Advisors, L.P., et al.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-31

    ... COMMISSION NGAM Advisors, L.P., et al.; Notice of Application July 25, 2013. AGENCY: Securities and Exchange... Advisors, L.P. (the ``Adviser''), Natixis ETF Trust (the ``Trust'') and NGAM Distribution, L.P. Filing... mail. Hearing requests should be received by the Commission by 5:30 p.m. on August 19, 2013 and...

  17. Roles and Responsibilities of International Student Advisors and Counselors in the United States.

    ERIC Educational Resources Information Center

    Dalili, Farid

    The separate but complementary roles of the international student advisor and counselor are discussed, and foreign student personnel services are addressed. It is suggested that the increasing number of international students indicates a need for a full-time foreign student advisor. The job is complicated by the different cultures and different…

  18. Working with Youth on LGBT Issues: Why Gay-Straight Alliance Advisors Become Involved

    ERIC Educational Resources Information Center

    Valenti, Maria; Campbell, Rebecca

    2009-01-01

    This phenomenological qualitative study explores motivation for citizen participation in a local context by exploring the experiences of Gay-Straight Alliance (GSA) club advisors from public high schools. Fourteen advisors from one midwestern state were randomly recruited for participation. Inductive and deductive qualitative analyses elucidated…

  19. The Advisor and Instructor as a Dynamic Duo: Academic Motivation and Basic Psychological Needs

    ERIC Educational Resources Information Center

    Burt, Tracie D.; Young-Jones, Adena D.; Yadon, Carly A.; Carr, Michael T.

    2013-01-01

    Students learn in and out of a formal classroom, and instructors and academic advisors play key roles in academic motivation and learning. Therefore, through the lens of self-determination theory, we examined the ways perceived support from instructors and advisors relates to satisfaction of college students' basic psychological needs.…

  20. Mentoring Support and Relational Uncertainty in the Advisor-Advisee Relationship

    ERIC Educational Resources Information Center

    Mansson, Daniel H.; Myers, Scott A.

    2013-01-01

    We examine the extent to which career mentoring and psychosocial mentoring received from their advisors relates to advisee perceptions of advisor-advisee relational uncertainty. Doctoral students (N = 378) completed the "Academic Mentoring Behaviors Scale" (Schrodt, Cawyer, & Sanders, 2003), the "Mentoring and Communication…

  1. 76 FR 75919 - President's Council of Advisors on Science and Technology Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-05

    ... TECHNOLOGY POLICY President's Council of Advisors on Science and Technology Meeting AGENCY: Office of Science... Council of Advisors on ] Science and Technology (PCAST) is an advisory group of the nation's leading scientists and engineers, appointed by the President to augment the science and technology advice...

  2. 78 FR 35617 - President's Council of Advisors on Science and Technology (PCAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-13

    ... President's Council of Advisors on Science and Technology (PCAST) AGENCY: Office of Science, Department of... Council of Advisors on Science and Technology (PCAST) is an advisory group of the nation's leading scientists and engineers, appointed by the President to augment the science and technology advice...

  3. 77 FR 74837 - President's Council of Advisors on Science and Technology (PCAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-18

    ... President's Council of Advisors on Science and Technology (PCAST) AGENCY: Office of Science, Department of... President's Council of Advisors on Science and Technology (PCAST) is an advisory group of the nation's leading scientists and engineers, appointed by the President to augment the science and technology...

  4. 75 FR 80045 - Federal Advisory Committee Act; Board of Advisors Charter Renewal

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-21

    ... COMMISSION Federal Advisory Committee Act; Board of Advisors Charter Renewal AGENCY: Election Assistance Commission. ACTION: Notice of Charter Renewal. SUMMARY: In accordance with the Federal Advisory Committee Act... (EAC) has renewed the charter for the Board of Advisors for a two-year period through December 14,...

  5. A Preliminary Report of Advisor Perceptions of Advising and of a Profession

    ERIC Educational Resources Information Center

    Aiken-Wisniewski, Sharon A.; Johnson, Anna; Larson, Joshua; Barkemeyer, Jason

    2015-01-01

    Practicing advisors may not agree, know, or understand that advising does not meet the scholarly definition of a profession. Through a phenomenological study, members of NACADA: The Global Community for Academic Advising were invited to describe the position of academic advisor. The data gathered were used to address two research questions:…

  6. 78 FR 29399 - ERNY Financial ETF Trust and ERNY Financial Advisors, LLC; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-20

    ... COMMISSION ERNY Financial ETF Trust and ERNY Financial Advisors, LLC; Notice of Application May 13, 2013... Financial ETF Trust (``Trust'') and ERNY Financial Advisors, LLC (``Adviser''). Summary of Application... and/or Sub-Advisers. \\4\\ Depositary Receipts are typically issued by a financial institution,...

  7. Using Mentoring Enactment Theory to Explore the Doctoral Student-Advisor Mentoring Relationship

    ERIC Educational Resources Information Center

    Mansson, Daniel H.; Myers, Scott A.

    2012-01-01

    The purposes of this study were (a) to develop a new measure to assess doctoral advisees' use of relational maintenance behaviors with their advisors, and (b) to examine both advisees' (n = 636) and advisors' (n = 141) perceptions of their mentoring relationship using mentoring enactment theory (MET; Kalbfleisch, 2002). The results of…

  8. GSA Advisors' Self-Efficacy Related to LGBT Youth of Color and Transgender Youth

    ERIC Educational Resources Information Center

    Poteat, V. Paul; Scheer, Jillian R.

    2016-01-01

    Gay-Straight Alliances (GSAs) serve lesbian, gay, bisexual, transgender (LGBT) and heterosexual youth from diverse backgrounds. Although some attention has been given to youth members in general, little attention has been given to adult advisors. Among 47 GSA advisors from 33 high schools (39 cisgender female, 8 cisgender male), the authors…

  9. Industrial-Organizational and Human Factors Graduate Program Admission: Information for Undergraduate Advisors

    ERIC Educational Resources Information Center

    Shoenfelt, Elizabeth L.; Stone, Nancy J.; Kottke, Janet L.

    2015-01-01

    Many psychology departments do not have industrial-organizational (IO) or human factors (HF) faculty members. As such, potential IO and HF graduate students may miss career opportunities because faculty advisors are unfamiliar with the disciplines and their graduate programs. To assist advisors, this article highlights the content of IO and HF…

  10. The Development of an International Student Advisor: A Grounded Theory Study

    ERIC Educational Resources Information Center

    Sparaco, Kathleen Keenan

    2012-01-01

    This qualitative study explored the professional experience of international student advisors. The statement of problem for this research was that the professional role of international student advisors has not been clearly defined or understood within U.S. higher education. The research questions asked (1) what encompassed the lived experience of…

  11. 78 FR 15057 - The Advisors' Inner Circle Fund, et al.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-08

    ... Capital Advisors, LLC (``PNC''), Frost Investment Advisors, LLC (``Frost''), GRT Capital Partners, L.L.C... series of AIC.\\4\\ Frost, a Delaware limited liability company and a wholly owned non- banking subsidiary of Frost Bank, is registered as an investment adviser under the Advisers Act and currently serves...

  12. Academic Advisee Motives for Pursuing Out-of-Class Communication with the Faculty Academic Advisor

    ERIC Educational Resources Information Center

    Leach, Rebecca B.; Wang, Tiffany R.

    2015-01-01

    This study examined advisee communication motives for engaging in out-of-class communication (OCC) with the faculty academic advisor. Undergraduate students (n = 21) were interviewed about their motives for engaging in OCC with their faculty academic advisors. In a thematic analysis, six motives emerged for engaging in OCC with faculty academic…

  13. Spectral-spatial hyperspectral classification based on multi-center SAM and MRF

    NASA Astrophysics Data System (ADS)

    Tang, Bo; Liu, Zhi; Xiao, Xiaoyan; Nie, Mingyu; Chang, Jun; Jiang, Wei; Li, Xiaomei; Zheng, Chengyun

    2015-12-01

    In this paper, a novel framework for an accurate spectral-spatial classification of hyperspectral images is proposed to address nonlinear classification problems. The algorithm is based on the spectral angle mapper (SAM), which is achieved by introducing the multi-center model and Markov random fields (MRF) into a probabilistic decision framework to obtain an accurate classification. Experimental comparisons between several traditional classification methods and the proposed MSAM-MRF algorithm have demonstrated that the performance of the proposed MSAM-MRF algorithm outperforms the traditional classification algorithms.

  14. [Preliminary study on autoregulation of samR involved in development and differentiation of Streptomyces ansochromogenes].

    PubMed

    Yang, Yan-ling; Yang, Hai-hua; Tan, Hua-rong

    2005-02-01

    The previous result showed that samR plays an important role in the development progress of Streptomyces ansochromogenes. It was reported that the differentiation progress of S. ansochromogenes was accelerated by a recombinant plasmid containing an extra copy of samR gene. However, the differentiation progress of S. ansochromogenes was not further accelerated by a multicopy plasmid containing samR gene. Electrophoresis mobility shift assay (EMSA) demonstrated that SamR binds to its own promoter region specifically. All these results hint that samR is an autoregulatory gene in Streptomyces ansochromogenes. PMID:15847153

  15. Leptin receptor activation increases Sam68 tyrosine phosphorylation and expression in human trophoblastic cells.

    PubMed

    Sánchez-Jiménez, Flora; Pérez-Pérez, Antonio; González-Yanes, Carmen; Najib, Souad; Varone, Cecilia L; Sánchez-Margalet, Víctor

    2011-01-30

    Leptin is produced in placenta where it has been found to be an important autocrine signal for trophoblastic growth during pregnancy, promoting antiapoptotic and trophic effects. Leptin receptor is present in trophoblastic cells and leptin may fully activate signaling. We have previously implicated the RNA-binding protein Sam68 in leptin signal transduction in immune cells. In the present work, we have studied the possible role of Sam68 in leptin receptor signaling in trophoblastic cells (JEG-3 cells). Leptin dose-dependently stimulated Sam68 phosphorylation in JEG-3 cells, as assessed by immunoprecipitation and immunoblot with anti-phosphotyrosine antibodies. As previously observed in other systems, tyrosine phosphorylation of Sam68 in response to leptin inhibits its RNA binding capacity. Besides, leptin stimulation dose-dependently increases Sam68 expression in JEG-3 cells, as assessed by quantitative PCR. Consistently, the amount of Sam68 protein is increased after 24h of leptin stimulation of trophoblastic cells. In order to study the possible role of Sam68 on leptin receptor synthesis, we employed antisense strategy to knockdown the expression of Sam68. We have found that a decrease in Sam68 expression leads to a decrease in leptin receptor amount in JEG-3 cells, as assessed both by quantitative PCR and immunoblot. These results strongly suggest the participation of Sam68 in leptin receptor signaling in human trophoblastic cells, and therefore, Sam68 may mediate some of the leptin effects in placenta. PMID:21035519

  16. First use of SAM onboard calibration gas cell

    NASA Astrophysics Data System (ADS)

    Malespin, C.; Trainer, M. G.; Manning, H. L.; Franz, H. B.; Conrad, P. G.; Raaen, E.; Webster, C. R.; Flesch, G.; Eigenbrode, J. L.; Wong, M. H.; Mahaffy, P. R.

    2015-12-01

    The Sample Analysis at Mars (SAM) instrument (Mahaffy et al 2012) suite on Curiosity completed its first measurement of the onboard calibration gas cell on MSL Mission Sol 1042. The cell consists of a gas mixture of four primary gases, along with trace fluorinated hydrocarbon high mass calibrants. The mix is comprised of approximately 25% CO2, N2, Xe and Ar, where the 129Xe has been given a three times enrichment relative to terrestrial xenon in order to distinguish it isotopically from Martian atmospheric Xe. Analysis of the calibration cell is intended to identify changes in instrument performance between pre-launch calibrations and operations on Mars, for any of the three main subsystems in SAM: the Quadrupole Mass Spectrometer (QMS), Tunable Laser Spectrometer (TLS), and Gas Chromatograph (GC). Here we present the experimental approach, results, and implications for instrument performance after almost three years of measurements on Mars.

  17. 17 CFR 5.4 - Applicability of part 4 of this chapter to commodity pool operators and commodity trading advisors.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... this chapter to commodity pool operators and commodity trading advisors. 5.4 Section 5.4 Commodity and... Applicability of part 4 of this chapter to commodity pool operators and commodity trading advisors. Part 4 of... commodity pool operator or as a commodity trading advisor. Failure by any such person to comply with...

  18. 17 CFR 5.4 - Applicability of part 4 of this chapter to commodity pool operators and commodity trading advisors.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... this chapter to commodity pool operators and commodity trading advisors. 5.4 Section 5.4 Commodity and... Applicability of part 4 of this chapter to commodity pool operators and commodity trading advisors. Part 4 of... commodity pool operator or as a commodity trading advisor. Failure by any such person to comply with...

  19. 17 CFR 5.4 - Applicability of part 4 of this chapter to commodity pool operators and commodity trading advisors.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... this chapter to commodity pool operators and commodity trading advisors. 5.4 Section 5.4 Commodity and... Applicability of part 4 of this chapter to commodity pool operators and commodity trading advisors. Part 4 of... commodity pool operator or as a commodity trading advisor. Failure by any such person to comply with...

  20. 17 CFR 5.4 - Applicability of part 4 of this chapter to commodity pool operators and commodity trading advisors.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... this chapter to commodity pool operators and commodity trading advisors. 5.4 Section 5.4 Commodity and... Applicability of part 4 of this chapter to commodity pool operators and commodity trading advisors. Part 4 of... commodity pool operator or as a commodity trading advisor. Failure by any such person to comply with...

  1. 77 FR 60732 - PACE Select Advisors Trust and UBS Global Asset Management (Americas) Inc.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-04

    ... COMMISSION PACE Select Advisors Trust and UBS Global Asset Management (Americas) Inc.; Notice of Application... requirements. The requested order would supersede two prior orders.\\1\\ \\1\\ PaineWebber PACE Select Advisors...) and 21666 (Jan. 11, 1996) (order). Applicants: PACE Select Advisors Trust (the ``Trust'') and...

  2. Advisor-Advisee Three: Graduate Students' Perceptions of Verbal Aggression, Credibility, and Conflict Styles in the Advising Relationship

    ERIC Educational Resources Information Center

    Punyanunt-Carter, Narissra M.; Wrench, Jason S.

    2008-01-01

    The current study looked at the relation between advisee perceptions of advisor's verbal aggression, credibility, and conflict styles. Participants were 153 graduate students who reported their perceptions concerning their advisor. First, the study found that advisee perceptions of their advisor's credibility (competence, caring/goodwill, &…

  3. The water quality of Sam Rayburn Reservoir, eastern Texas

    USGS Publications Warehouse

    Rawson, Jack; Lansford, Myra W.

    1971-01-01

    Results of periodic surveys indicate that dissolved-oxygen concentrations at three sites in the 19-mile reach of the Angelina River downstream from Sam Rayburn Dam were low in late summer and early fall after periods of summer stagnation in the reservoir. Moreover, the amount of reaeration that occurred in the reach was insignificant. During periods when the dissolved-oxygen deficiency was large, the concentrations of iron and manganese at each of the three sites increased greatly.

  4. GLAO4ELT: trade study and SAM experience

    NASA Astrophysics Data System (ADS)

    Tokovinin, A.

    2011-09-01

    It is expected that a large fraction of wave-front distortions at ELTs will be anisoplanatic, being produced jointly by near-ground and dome turbulence, wind buffeting, flexures, and vibrations. We compare various strategies for sensing this "ground" component using (i) several sodium LGSs, (ii) one or several Rayleigh LGSs and (ii) NGSs in a wide field. The experience gained so far with the RLGS GLAO system SAM at 4-m telescope will be presented and discussed.

  5. SAMS Acceleration Measurements on Mir (NASA Increment 4)

    NASA Technical Reports Server (NTRS)

    DeLombard, Richard

    1998-01-01

    During NASA Increment 4 (January to May 1997), about 5 gigabytes of acceleration data were collected by the Space Acceleration Measurements System (SAMS) onboard the Russian Space Station, Mir. The data were recorded on 28 optical disks which were returned to Earth on STS-84. During this increment, SAMS data were collected in the Priroda module to support the Mir Structural Dynamics Experiment (MiSDE), the Binary Colloidal Alloy Tests (BCAT), Angular Liquid Bridge (ALB), Candle Flames in Microgravity (CFM), Diffusion Controlled Apparatus Module (DCAM), Enhanced Dynamic Load Sensors (EDLS), Forced Flow Flame Spreading Test (FFFr), Liquid Metal Diffusion (LMD), Protein Crystal Growth in Dewar (PCG/Dewar), Queen's University Experiments in Liquid Diffusion (QUELD), and Technical Evaluation of MIM (TEM). This report points out some of the salient features of the microgravity environment to which these experiments were exposed. Also documented are mission events of interest such as the docked phase of STS-84 operations, a Progress engine bum, Soyuz vehicle docking and undocking, and Progress vehicle docking. This report presents an overview of the SAMS acceleration measurements recorded by 10 Hz and 100 Hz sensor heads. The analyses included herein complement those presented in previous summary reports prepared by the Principal Investigator Microgravity Services (PIMS) group.

  6. Mechanistic Enzymology of the Radical SAM Enzyme DesII

    PubMed Central

    2016-01-01

    DesII is a member of the radical SAM family of enzymes that catalyzes radical-mediated transformations of TDP-4-amino-4,6-didexoy-D-glucose as well as other sugar nucleotide diphosphates. Like nearly all radical SAM enzymes, the reactions begin with the reductive homolysis of SAM to produce a 5′-deoxyadenosyl radical which is followed by regiospecific hydrogen atom abstraction from the substrate. What happens next, however, depends on the nature of the substrate radical so produced. In the case of the biosynthetically relevant substrate, a radical-mediated deamination ensues; however, when this amino group is replaced with a hydroxyl, one instead observes dehydrogenation. The factors that govern the fate of the initially generated substrate radical as well as the mechanistic details underlying these transformations have been a key focus of research into the chemistry of DesII. This review will discuss recent discoveries pertaining to the enzymology of DesII, how it may relate to understanding other radical-mediated lyases and dehydrogenases and the working hypotheses currently being investigated regarding the mechanism of DesII catalysis.

  7. Deposition of DNA rafts on cationic SAMs on silicon [100].

    PubMed

    Sarveswaran, Koshala; Hu, Wenchuang; Huber, Paul W; Bernstein, Gary H; Lieberman, Marya

    2006-12-19

    We demonstrate a guided self-assembly approach to the fabrication of DNA nanostructures on silicon substrates. DNA oligonucleotides self-assemble into "rafts" 8 x 37 x 2 nm in size. The rafts bind to cationic SAMs on silicon wafers. Electron-beam lithography of a thin poly(methyl methacrylate) (PMMA) resist layer was used to define trenches, and (3-aminopropyl)triethoxysilane (APTES), a cationic SAM precursor, was deposited from aqueous solution onto the exposed silicon dioxide at the trench bottoms. The remaining PMMA can be cleanly stripped off with dichloromethane, leaving APTES layers 0.7-1.2 nm in thickness and 110 nm in width. DNA rafts bind selectively to the resulting APTES stripes. The coverage of DNA rafts on adjacent areas of silicon dioxide is 20 times lower than on the APTES stripes. The topographic features of the rafts, measured by AFM, are identical to those of rafts deposited on wide-area SAMs. Binding to the APTES stripes appears to be very strong as indicated by "jamming" of the rafts at a saturation coverage of 42% and the stability to repeated AFM scanning in air.

  8. CE-SAM: a conversational interface for ISR mission support

    NASA Astrophysics Data System (ADS)

    Pizzocaro, Diego; Parizas, Christos; Preece, Alun; Braines, Dave; Mott, David; Bakdash, Jonathan Z.

    2013-05-01

    There is considerable interest in natural language conversational interfaces. These allow for complex user interactions with systems, such as fulfilling information requirements in dynamic environments, without requiring extensive training or a technical background (e.g. in formal query languages or schemas). To leverage the advantages of conversational interactions we propose CE-SAM (Controlled English Sensor Assignment to Missions), a system that guides users through refining and satisfying their information needs in the context of Intelligence, Surveillance, and Reconnaissance (ISR) operations. The rapidly-increasing availability of sensing assets and other information sources poses substantial challenges to effective ISR resource management. In a coalition context, the problem is even more complex, because assets may be "owned" by different partners. We show how CE-SAM allows a user to refine and relate their ISR information needs to pre-existing concepts in an ISR knowledge base, via conversational interaction implemented on a tablet device. The knowledge base is represented using Controlled English (CE) - a form of controlled natural language that is both human-readable and machine processable (i.e. can be used to implement automated reasoning). Users interact with the CE-SAM conversational interface using natural language, which the system converts to CE for feeding-back to the user for confirmation (e.g. to reduce misunderstanding). We show that this process not only allows users to access the assets that can support their mission needs, but also assists them in extending the CE knowledge base with new concepts.

  9. In situ analysis of martian regolith with the SAM experiment during the first mars year of the MSL mission: Identification of organic molecules by gas chromatography from laboratory measurements

    NASA Astrophysics Data System (ADS)

    Millan, M.; Szopa, C.; Buch, A.; Coll, P.; Glavin, D. P.; Freissinet, C.; Navarro-Gonzalez, R.; François, P.; Coscia, D.; Bonnet, J. Y.; Teinturier, S.; Cabane, M.; Mahaffy, P. R.

    2016-09-01

    The Sample Analysis at Mars (SAM) instrument onboard the Curiosity rover, is specifically designed for in situ molecular and isotopic analyses of martian surface materials and atmosphere. It contributes to the Mars Science Laboratory (MSL) missions primary scientific goal to characterize the potential past, present or future habitability of Mars. In all of the analyses of solid samples delivered to SAM so far, chlorinated organic compounds have been detected above instrument background levels and identified by gas chromatography coupled to mass spectrometry (GC-MS) (Freissinet et al., 2015; Glavin et al., 2013). While some of these may originate from reactions between oxychlorines and terrestrial organic carbon present in the instrument background (Glavin et al., 2013), others have been demonstrated to originate from indigenous organic carbon present in samples (Freissinet et al., 2015). We present here laboratory calibrations that focused on the analyses performed with the MXT-CLP GC column (SAM GC-5 channel) used for nearly all of the GC-MS analyses of the martian soil samples carried out with SAM to date. Complementary to the mass spectrometric data, gas chromatography allows us to separate and identify the species analyzable in a nominal SAM-GC run time of about 21 min. To characterize the analytical capabilities of this channel within the SAM Flight Model (FM) operating conditions on Mars, and their implications on the detection of organic matter, it is required to perform laboratory experimental tests and calibrations on spare model components. This work assesses the SAM flight GC-5 column efficiency, confirms the identification of the molecules based on their retention time, and enables a better understanding of the behavior of the SAM injection trap (IT) and its release of organic molecules. This work will enable further optimization of the SAM-GC runs for additional samples to be analyzed during the MSL mission.

  10. The SAMS: Smartphone Addiction Management System and verification.

    PubMed

    Lee, Heyoung; Ahn, Heejune; Choi, Samwook; Choi, Wanbok

    2014-01-01

    While the popularity of smartphones has given enormous convenience to our lives, their pathological use has created a new mental health concern among the community. Hence, intensive research is being conducted on the etiology and treatment of the condition. However, the traditional clinical approach based surveys and interviews has serious limitations: health professionals cannot perform continual assessment and intervention for the affected group and the subjectivity of assessment is questionable. To cope with these limitations, a comprehensive ICT (Information and Communications Technology) system called SAMS (Smartphone Addiction Management System) is developed for objective assessment and intervention. The SAMS system consists of an Android smartphone application and a web application server. The SAMS client monitors the user's application usage together with GPS location and Internet access location, and transmits the data to the SAMS server. The SAMS server stores the usage data and performs key statistical data analysis and usage intervention according to the clinicians' decision. To verify the reliability and efficacy of the developed system, a comparison study with survey-based screening with the K-SAS (Korean Smartphone Addiction Scale) as well as self-field trials is performed. The comparison study is done using usage data from 14 users who are 19 to 50 year old adults that left at least 1 week usage logs and completed the survey questionnaires. The field trial fully verified the accuracy of the time, location, and Internet access information in the usage measurement and the reliability of the system operation over more than 2 weeks. The comparison study showed that daily use count has a strong correlation with K-SAS scores, whereas daily use times do not strongly correlate for potentially addicted users. The correlation coefficients of count and times with total K-SAS score are CC = 0.62 and CC =0.07, respectively, and the t-test analysis for the

  11. Scientific writing: strategies and tools for students and advisors.

    PubMed

    Singh, Vikash; Mayer, Philipp

    2014-01-01

    Scientific writing is a demanding task and many students need more time than expected to finish their research articles. To speed up the process, we highlight some tools, strategies as well as writing guides. We recommend starting early in the research process with writing and to prepare research articles, not after but in parallel to the lab or field work. We suggest considering scientific writing as a team enterprise, which needs proper organization and regular feedback. In addition, it is helpful to select potential target journals early and to consider not only scope and reputation, but also decision times and rejection rates. Before submission, instructions to authors and writing guides should be considered, and drafts should be extensively revised. Later in the process editor's and reviewer's comments should be followed. Our tips and tools help students and advisors to structure the writing and publishing process, thereby stimulating them to develop their own strategies to success.

  12. WinMod: An expert advisor for investment casting

    SciTech Connect

    Bivens, H.P.; Williamson, G.A. Jr.; Luger, G.F.; Erdmann, R.G.; Maguire, M.C.; Baldwin, M.D.; Anderson, D.J.

    1998-04-01

    Investment casting is an important method for fabricating a variety of high quality components in mechanical systems. Cast components, unfortunately, have a large design and gate/runner build time associated with their fabrication. In addition, casting engineers often require many years of actual experience in order to consistently pour high quality castings. Since 1989, Sandia National Laboratories has been investigating casting technology and software that will reduce the time overhead involved in producing quality casts. Several companies in the casting industry have teamed up with Sandia to form the FASTCAST Consortium. One result of this research and the formation of the FASTCAST consortium is the creation of the WinMod software, an expert casting advisor that supports the decision making process of the casting engineer through visualization and advice to help eliminate possible casting defects.

  13. Multi-Center Traffic Management Advisor Operational Field Test Results

    NASA Technical Reports Server (NTRS)

    Farley, Todd; Landry, Steven J.; Hoang, Ty; Nickelson, Monicarol; Levin, Kerry M.; Rowe, Dennis W.

    2005-01-01

    The Multi-Center Traffic Management Advisor (McTMA) is a research prototype system which seeks to bring time-based metering into the mainstream of air traffic control (ATC) operations. Time-based metering is an efficient alternative to traditional air traffic management techniques such as distance-based spacing (miles-in-trail spacing) and managed arrival reservoirs (airborne holding). While time-based metering has demonstrated significant benefit in terms of arrival throughput and arrival delay, its use to date has been limited to arrival operations at just nine airports nationally. Wide-scale adoption of time-based metering has been hampered, in part, by the limited scalability of metering automation. In order to realize the full spectrum of efficiency benefits possible with time-based metering, a much more modular, scalable time-based metering capability is required. With its distributed metering architecture, multi-center TMA offers such a capability.

  14. Eos Interviews John Holdren, President Obama's Science Advisor

    NASA Astrophysics Data System (ADS)

    Showstack, Randy

    2010-12-01

    With the Obama administration about to face a Republican-led House of Representatives in January, presidential science advisor John Holdren sat down with Eos for an exclusive and wide-ranging interview following a policy speech he delivered on 13 December at the AGU Fall Meeting in San Francisco. During the interview, Holdren, who also is director of the White House Office of Science and Technology Policy (OSTP), focused on the challenging congressional and budgetary environment, the administration's priorities related to the Earth sciences, and the responsibility of scientists in helping to communicate the societal benefits of science, educate the public, and improve science education and literacy. Holdren said the Obama administration's top priorities related to the Earth sciences include improving observations of the Earth, making progress in dealing with climate change, and rebalancing NASA's focus.

  15. Science Advisor and Applied Physicist: Joseph Henry Serves His Country

    NASA Astrophysics Data System (ADS)

    Rothenberg, Marc

    1997-04-01

    When Joseph Henry accepted the postion of secretary of the Smithsonian in 1846, his career changed radically. Although he never ceased thinking of himself as a research scientist and educator, thereafter his chief roles were those of science administrator and advisor to both the executive and legislative branches of the federal government for both science and technology. His effectiveness as an advocate became more important than his skill as an experimental physicist. Even when he entered the laboratory, his role had changed. No longer was he concerned with basic research. As a member of various government boards and committees, Henry spent the last three decades of his life concerned with the application of fundamental knowledge for the improvement of the human condition. This paper will discuss Henry's service on behalf of his country.

  16. [Senescence-accelerated mouse (SAM): with special reference to age-associated pathologies and their modulation].

    PubMed

    Takeda, T

    1996-07-01

    The senescence-accelerated mouse (SAM) has been under development by our research team at Kyoto University since 1970 through selective inbreeding of the AKR/J strain of mice donated by the Jackson Laboratory in 1968, based on the data of the grading score of senescence, life span, and pathologic phenotypes. At present, there are 12 lines of SAM; the 9 senescence-prone inbred strains (SAMP) include SAMP1, SAMP2, SAMP3, SAMP6, SAMP7, SAMP8, SAMP9, SAMP10 and SAMP11, and the 3 senescence-resistant inbred strains (SAMR) SAMR1, SANR4 and SAMR5. Data from survival curves, the Gompertzian function and the grading score of senescence, together with growth patterns of body weight of these SAMP and SAMR mice revealed that the characteristic feature of aging common to all SAMP mice is "accelerated senescence": early onset and irreversible advance of senescence manifested by several signs and gross lesions such as the loss of normal behavior, various skin lesions, increased lordokyphosis, etc., after a period of normal development. Routine postmortem examinations and the pathobiological features revealed by systematically designed studies have shown several pathologic phenotypes, which are often characteristic enough to differentiate among the various SAM strains: senile amyloidosis in SAMP1, -P2, -P7, -P9, -P10 and -P11, secondary amyloidosis in SAMP2 and -P6, contracted kidney in SAMP1, -P2, -P10, -P11, immunoblastic lymphoma in SAMR1 and -R4, histiocytic sarcoma in SAMR1 and -R4, ovarian cysts in SAMR1, impaired immune response in SAMP1, -P2 and -P8, hyperinflation of the lungs in SAMP1, hearing impairment in SAMP1, degenerative temporomandibular joint disease in SAMP3, senile osteoporosis in SAMP6, deficits in learning and memory in SAMP8 and -P10, emotional disorders in SAMP8 and -P10, cataracts in SAMP9, and brain atrophy in SAMP10. These are all age-associated pathologies, the incidence and severity of which increase with advancing age. The SAM model in which these

  17. Unliganded EphA3 dimerization promoted by the SAM domain

    NASA Astrophysics Data System (ADS)

    Hristova, Kalina; Singh, Deo; King, Christopher; Ahmed, Fozia; Pasquale, Elena

    The EphA3 receptor tyrosine kinase regulates morphogenesis during development, and is overexpressed and mutated in a variety of cancers. EphA3 activation is believed to follow a ``seeding mechanism'' model, in which ligand binding to the monomeric receptor acts as a trigger for signal-productive receptor clustering. We use a novel approach to study EphA3 lateral interactions on the surface of live cells, and we demonstrate that EphA3 forms dimers in the absence of ligand binding. We further show that these dimers are stabilized by interactions involving the EphA3 SAM domain. The discovery of unliganded EphA3 dimers challenges the current understanding of the chain of EphA3 activation events, and suggests that EphA3 may follow the ``pre-formed dimer'' model of activation known to be relevant for other receptor tyrosine kinases. This work also establishes a new role for the SAM domain in promoting Eph receptor lateral interactions and signaling on the cell surface.

  18. Unliganded EphA3 dimerization promoted by the SAM domain

    PubMed Central

    Singh, Deo R.; Cao, QingQing; King, Christopher; Salotto, Matt; Ahmed, Fozia; Zhou, Xiang Yang; Pasquale, Elena B.; Hristova, Kalina

    2015-01-01

    The erythropoietin-producing hepatocellular carcinoma A3 (EphA3) receptor tyrosine kinase (RTK) regulates morphogenesis during development and is overexpressed and mutated in a variety of cancers. EphA3 activation is believed to follow a ‘seeding mechanism’ model, in which ligand binding to the monomeric receptor acts as a trigger for signal-productive receptor clustering. We study EphA3 lateral interactions on the surface of live cells and we demonstrate that EphA3 forms dimers in the absence of ligand binding. We further show that these dimers are stabilized by interactions involving the EphA3 sterile α-motif (SAM) domain. The discovery of unliganded EphA3 dimers challenges the current understanding of the chain of EphA3 activation events and suggests that EphA3 may follow the ‘pre-formed dimer’ model of activation known to be relevant for other receptor tyrosine kinases. The present work also establishes a new role for the SAM domain in promoting Eph receptor lateral interactions and signalling on the cell surface. PMID:26232493

  19. NREL's System Advisor Model Simplifies Complex Energy Analysis (Fact Sheet)

    SciTech Connect

    Not Available

    2011-10-01

    The energy market is diversifying. In addition to traditional power sources, decision makers can choose among solar, wind, and geothermal technologies as well. Each of these technologies has complex performance characteristics and economics that vary with location and other project specifics, making it difficult to analyze the viability of such projects. But that analysis is easier now, thanks to the National Renewable Energy Laboratory (NREL).

  20. Systematic biochemical characterization of the SAM domains in Eph receptor family from Mus Musculus.

    PubMed

    Wang, Yue; Li, Qingxia; Zheng, Yunhua; Li, Gang; Liu, Wei

    2016-05-13

    The Eph receptor family is the largest subfamily of receptor tyrosine kinases and well-known for their pivotal roles in axon guidance, synaptogenesis, artery/venous differentiation and tumorigenesis, etc. Activation of the Eph receptor needs multimerization of the receptors. The intracellular C-terminal SAM domain of Eph receptor was reported to mediate self-association of Eph receptors via the homo SAM-SAM interaction. In this study, we systematically expressed and purified the SAM domain proteins of all fourteen Eph receptors of Mus musculus in Escherichia coli. The FPLC (fast protein liquid chromatography) results showed the recombinant SAM domains were highly homogeneous. Using CD (circular dichroism) spectrometry, we found that the secondary structure of all the SAM domains was typically alpha helical folded and remarkably similar. The thermo-stability tests showed that they were quite stable in solution. SEC-MALS (size exclusion chromatography coupled with multiple angle light scattering) results illustrated 200 μM Eph SAM domains behaved as good monomers in the size-exclusion chromatography. More importantly, DLS (dynamic light scattering) results revealed the overwhelming majority of SAM domains was not multimerized in solution either at 200 μM or 2000 μM protein concentration, which indicating the SAM domain alone was not sufficient to mediate the polymerization of Eph receptor. In summary, our studies provided the systematic biochemical characterizations of the Eph receptor SAM domains and implied their roles in Eph receptor mediated signaling pathways. PMID:27086853

  1. An intelligent, knowledge-based multiple criteria decision making advisor for systems design

    NASA Astrophysics Data System (ADS)

    Li, Yongchang

    In systems engineering, design and operation of systems are two main problems which always attract researcher's attentions. The accomplishment of activities in these problems often requires proper decisions to be made so that the desired goal can be achieved, thus, decision making needs to be carefully fulfilled in the design and operation of systems. Design is a decision making process which permeates through out the design process, and is at the core of all design activities. In modern aircraft design, more and more attention is paid to the conceptual and preliminary design phases so as to increase the odds of choosing a design that will ultimately be successful at the completion of the design process, therefore, decisions made during these early design stages play a critical role in determining the success of a design. Since aerospace systems are complex systems with interacting disciplines and technologies, the Decision Makers (DMs) dealing with such design problems are involved in balancing the multiple, potentially conflicting attributes/criteria, transforming a large amount of customer supplied guidelines into a solidly defined set of requirement definitions. Thus, one could state with confidence that modern aerospace system design is a Multiple Criteria Decision Making (MCDM) process. A variety of existing decision making methods are available to deal with this type of decision problems. The selection of the most appropriate decision making method is of particular importance since inappropriate decision methods are likely causes of misleading engineering design decisions. With no sufficient knowledge about each of the methods, it is usually difficult for the DMs to find an appropriate analytical model capable of solving their problems. In addition, with the complexity of the decision problem and the demand for more capable methods increasing, new decision making methods are emerging with time. These various methods exacerbate the difficulty of the selection

  2. [Glimpsing undergraduate research from the view of the advisors of Nursing scholarships].

    PubMed

    Erdmann, Alacoque Lorenzini; Leite, Joséte Luzia; Nascimento, Keyla Cristiane do; Lanzoni, Gabriela Marcellino de Melo

    2011-01-01

    This research aimed at understanding the meaning of undergraduate research for supervisors of Nursing scholarship students in a university in the South of Brazil. The methodological reference used was the Grounded Theory, by the means of interviews with seven undergraduate research scholarship advisors forming two sample groups. The phenomenon "glimpsing undergraduate research activities of research groups coordinated by nursing advisors, the basis of competency formation in research of the scholarships" emerged form the interrelation of six categories. To be a advisor and researcher of human resources in research form undergraduation requires pedagogical, instrumental, and managerial competencies associated to research policies of nursing and health.

  3. Shear-Induced Detachment of Polystyrene Beads from SAM-Coated Surfaces.

    PubMed

    Cho, Kwun Lun; Rosenhahn, Axel; Thelen, Richard; Grunze, Michael; Lobban, Matthew; Karahka, Markus Leopold; Kreuzer, H Jürgen

    2015-10-13

    In this work we experimentally and theoretically analyze the detachment of microscopic polystyrene beads from different self-assembled monolayer (SAM) surfaces in a shear flow in order to develop a mechanistic model for the removal of cells from surfaces. The detachment of the beads from the surface is treated as a thermally activated process applying an Arrhenius Ansatz to determine the activation barrier and attempt frequency of the rate determing step in bead removal. The statistical analysis of the experimental shear detachment data obtained in phosphate-buffered saline buffer results in an activation energy around 20 kJ/mol, which is orders of magnitude lower than the adhesion energy measured by atomic force microscopy (AFM). The same order of magnitude for the adhesion energy measured by AFM is derived from ab initio calculations of the van der Waals interaction energy between the polystyrene beads and the SAM-covered gold surface. We conclude that the rate determing step for detachment of the beads is the initiation of rolling on the surface (overcoming static friction) and not physical detachment, i.e., lifting the particle off the surface. PMID:26401759

  4. Shear-Induced Detachment of Polystyrene Beads from SAM-Coated Surfaces.

    PubMed

    Cho, Kwun Lun; Rosenhahn, Axel; Thelen, Richard; Grunze, Michael; Lobban, Matthew; Karahka, Markus Leopold; Kreuzer, H Jürgen

    2015-10-13

    In this work we experimentally and theoretically analyze the detachment of microscopic polystyrene beads from different self-assembled monolayer (SAM) surfaces in a shear flow in order to develop a mechanistic model for the removal of cells from surfaces. The detachment of the beads from the surface is treated as a thermally activated process applying an Arrhenius Ansatz to determine the activation barrier and attempt frequency of the rate determing step in bead removal. The statistical analysis of the experimental shear detachment data obtained in phosphate-buffered saline buffer results in an activation energy around 20 kJ/mol, which is orders of magnitude lower than the adhesion energy measured by atomic force microscopy (AFM). The same order of magnitude for the adhesion energy measured by AFM is derived from ab initio calculations of the van der Waals interaction energy between the polystyrene beads and the SAM-covered gold surface. We conclude that the rate determing step for detachment of the beads is the initiation of rolling on the surface (overcoming static friction) and not physical detachment, i.e., lifting the particle off the surface.

  5. Measuring the conformational space of square four-helical bundles with the program samCC.

    PubMed

    Dunin-Horkawicz, Stanislaw; Lupas, Andrei N

    2010-05-01

    Four-helical bundles are the most abundant topological motif among helical folds. Their constituent helices show crossing angles that mainly cluster around +20 degrees (aligned) or -50 degrees (orthogonal). Bundles with all helices aligned are called 'square' and comprise four-helical coiled coils as their structurally most regular form. Since coiled coils can be described fully by parametric equations, they can serve as a reference point for quantifying the conformational space of all square bundles. To this end we have developed a program, samCC, which measures the deviation of a given bundle from an idealized coiled coil and decomposes this into axial rotation and axial, radial, and angular shifts. We present examples of analyses performed with the program and focus in particular on the axial rotation states of helices in coiled coils, in order to gain further insight into a proposed mechanism for transmembrane signal transduction, which involves a 26 degrees axial rotation of helices between a canonical coiled coil and a variant called the Alacoil. We find that, unlike expected from the mechanistic model, coiled coils show a continuum of axial rotation states, suggesting that the Alacoil does not represent a single, defined state. We also find that one of the originally proposed Alacoil proteins, Rop, in fact has canonical packing. SamCC is freely available as a web service athttp://toolkit.tuebingen.mpg.de/samcc.

  6. Benchmark Simulations of the Thermal-Hydraulic Responses during EBR-II Inherent Safety Tests using SAM

    SciTech Connect

    Hu, Rui; Sumner, Tyler S.

    2016-01-01

    An advanced system analysis tool SAM is being developed for fast-running, improved-fidelity, and wholeplant transient analyses at Argonne National Laboratory under DOE-NE’s Nuclear Energy Advanced Modeling and Simulation (NEAMS) program. As an important part of code development, companion validation activities are being conducted to ensure the performance and validity of the SAM code. This paper presents the benchmark simulations of two EBR-II tests, SHRT-45R and BOP- 302R, whose data are available through the support of DOE-NE’s Advanced Reactor Technology (ART) program. The code predictions of major primary coolant system parameter are compared with the test results. Additionally, the SAS4A/SASSYS-1 code simulation results are also included for a code-to-code comparison.

  7. Detection of Nitric Oxide by the Sample Analysis at Mars (SAM) Instrument Implications for the Presence of Nitrates

    NASA Technical Reports Server (NTRS)

    Navarro-Gonzalez, R.; Stern, J.; Freissinet, C.; Franz, H. B.; Eigenbrode, J. L..; McKay, C. P.; Coll, P.; Sutter, B.; Archer, D.; McAdam, A.; Cabane, M.; Ming, D. W.; Glavin, D.; Leshin, L.; Wong, M.; Atreya, S.; Wray, J. J.; Steele, A.; Buch, A.; Prats, B. D.; Szopa, C.; Coscia, D.; Teinturier, S.; Conrad, P.; Owen, T. C.; Mahaffy, P.; Grotzinger, J. P.

    2014-01-01

    One of the main goals of the Mars Science Laboratory is to determine whether the planet ever had environmental conditions able to support microbial life. Nitrogen is a fundamental element for life, and is present in structural (e.g., proteins), catalytic (e.g., enzymes and ribozymes), energy transfer (e.g., ATP) and information storage (RNA and DNA) biomolecules. Planetary models suggest that molecular nitrogen was abundant in the early Martian atmosphere, but was rapidly lost to space by photochemistry, sputtering impact erosion, and oxidized and deposited to the surface as nitrate. Nitrates are a fundamental source for nitrogen to terrestrial microorganisms. Therefore, the detection of nitrates in soils and rocks is important to assess the habitability of a Martian environment. SAM is capable of detecting nitrates by their thermal decomposition into nitric oxide, NO. Here we analyze the release of NO from soils and rocks examined by the SAM instrument at Gale crater, and discuss its origin.

  8. Non-canonical active site architecture of the radical SAM thiamin pyrimidine synthase.

    PubMed

    Fenwick, Michael K; Mehta, Angad P; Zhang, Yang; Abdelwahed, Sameh H; Begley, Tadhg P; Ealick, Steven E

    2015-03-27

    Radical S-adenosylmethionine (SAM) enzymes use a [4Fe-4S] cluster to generate a 5'-deoxyadenosyl radical. Canonical radical SAM enzymes are characterized by a β-barrel-like fold and SAM anchors to the differentiated iron of the cluster, which is located near the amino terminus and within the β-barrel, through its amino and carboxylate groups. Here we show that ThiC, the thiamin pyrimidine synthase in plants and bacteria, contains a tethered cluster-binding domain at its carboxy terminus that moves in and out of the active site during catalysis. In contrast to canonical radical SAM enzymes, we predict that SAM anchors to an additional active site metal through its amino and carboxylate groups. Superimposition of the catalytic domains of ThiC and glutamate mutase shows that these two enzymes share similar active site architectures, thus providing strong evidence for an evolutionary link between the radical SAM and adenosylcobalamin-dependent enzyme superfamilies.

  9. Non-canonical active site architecture of the radical SAM thiamin pyrimidine synthase

    SciTech Connect

    Fenwick, Michael K.; Mehta, Angad P.; Zhang, Yang; Abdelwahed, Sameh H.; Begley, Tadhg P.; Ealick, Steven E.

    2015-03-27

    Radical S-adenosylmethionine (SAM) enzymes use a [4Fe-4S] cluster to generate a 5'-deoxyadenosyl radical. Canonical radical SAM enzymes are characterized by a β-barrel-like fold and SAM anchors to the differentiated iron of the cluster, which is located near the amino terminus and within the β-barrel, through its amino and carboxylate groups. Here we show that ThiC, the thiamin pyrimidine synthase in plants and bacteria, contains a tethered cluster-binding domain at its carboxy terminus that moves in and out of the active site during catalysis. In contrast to canonical radical SAM enzymes, we predict that SAM anchors to an additional active site metal through its amino and carboxylate groups. Superimposition of the catalytic domains of ThiC and glutamate mutase shows that these two enzymes share similar active site architectures, thus providing strong evidence for an evolutionary link between the radical SAM and adenosylcobalamin-dependent enzyme superfamilies.

  10. Localization of event-related activity by SAM(erf).

    PubMed

    Robinson, S E

    2004-01-01

    Synthetic aperture magnetometry (SAM) has been used to image source power or source signal-to-noise ratio from MEG. However, the locations of maximal event-related oscillatory activity (or changes from resting state) do not necessarily coincide with those sites that are phase-locked to external events (i.e., localized by dipole fit to the averaged evoked response). Since an estimate of the source time-series may also be obtained by applying the beamformer coefficients to the MEG signal, one can image event-related activity by mapping some function reflecting the reliability of the averaged source waveform at each location. We have devised a new analysis method, SAM(erf), for obtaining a functional image of event-related brain activity and revealing the corresponding waveforms for activated sites. The mapping function used is the ratio of RMS amplitude of the averaged source waveform to that of the +/- average waveform, for a selected time window. This function is computed at each coordinate on a three-dimensional grid in the head. In addition to the SAM(erf) functional image, the averaged source waveforms for each local maximum in the image can be computed and displayed. This procedure can reveal multiple locations and waveforms at sites in the brain engaged in event-related activities. When this method is applied to evoked response studies, phase-locked activity can sometimes be found in areas distant from primary sensory cortex. Given the sensitivity of this functional imaging method to areas outside primary sensory cortex, it has the potential for detecting subtle changes in brain activity in health and disease. PMID:16012649

  11. Paramagnetic Intermediates Generated by Radical S-Adenosylmethionine (SAM) Enzymes

    PubMed Central

    2015-01-01

    Conspectus A [4Fe–4S]+ cluster reduces a bound S-adenosylmethionine (SAM) molecule, cleaving it into methionine and a 5′-deoxyadenosyl radical (5′-dA•). This step initiates the varied chemistry catalyzed by each of the so-called radical SAM enzymes. The strongly oxidizing 5′-dA• is quenched by abstracting a H-atom from a target species. In some cases, this species is an exogenous molecule of substrate, for example, l-tyrosine in the [FeFe] hydrogenase maturase, HydG. In other cases, the target is a proteinaceous residue as in all the glycyl radical forming enzymes. The generation of this initial radical species and the subsequent chemistry involving downstream radical intermediates is meticulously controlled by the enzyme so as to prevent unwanted reactions. But the manner in which this control is exerted is unknown. Electron paramagnetic resonance (EPR) spectroscopy has proven to be a valuable tool used to gain insight into these mechanisms. In this Account, we summarize efforts to trap such radical intermediates in radical SAM enzymes and highlight four examples in which EPR spectroscopic results have shed significant light on the corresponding mechanism. For lysine 2,3-aminomutase, nearly each possible intermediate, from an analogue of the initial 5′-dA• to the product radical l-β-lysine, has been explored. A paramagnetic intermediate observed in biotin synthase is shown to involve an auxiliary [FeS] cluster whose bridging sulfide is a co-substrate for the final step in the biosynthesis of vitamin B7. In HydG, the l-tyrosine substrate is converted in unprecedented fashion to a 4-oxidobenzyl radical on the way to generating CO and CN– ligands for the [FeFe] cluster of hydrogenase. And finally, EPR has confirmed a mechanistic proposal for the antibiotic resistance protein Cfr, which methylates the unactivated sp2-hybridized C8-carbon of an adenosine base of 23S ribosomal RNA. These four systems provide just a brief survey of the ever-growing set

  12. Comparative studies of aerosol extinction measurements made by the SAM II and SAGE II satellite experiments

    NASA Technical Reports Server (NTRS)

    Yue, Glenn K.; Mccormick, M. P.; Chu, W. P.; Wang, P.; Osborn, M. T.

    1989-01-01

    Results from the Stratospheric Aerosol Measurement (SAM) II and Stratospheric Aerosol and Gas Experiment (SAGE) II are compared for measurement locations which are coincident in time and space. At 1.0 micron, the SAM II and SAGE II aerosol extinction profiles are similar within their measurement errors. In addition, sunrise and sunset aerosol extinction data at four different wavelengths are compared for occasions when the SAGE II and SAM II measurements are nearly coincident in space and about 12 hours apart.

  13. Regulation of gene expression by the RNA-binding protein Sam68 in cancer.

    PubMed

    Rajan, Prabhakar; Gaughan, Luke; Dalgliesh, Caroline; El-Sherif, Amira; Robson, Craig N; Leung, Hing Y; Elliott, David J

    2008-06-01

    Sam68 (Src-associated in mitosis 68 kDa) is the prototypical member of the STAR (signal transducer and activator of RNA) family of RNA-binding proteins. Sam68 is implicated in a number of cellular processes including signal transduction, transcription, RNA metabolism, cell cycle regulation and apoptosis. In the present review, we summarize the functions of Sam68 as a transcriptional and post-transcriptional regulator of gene expression, with particular relevance to cancer. PMID:18481990

  14. The nuclear protein Sam68 is cleaved by the FMDV 3C protease redistributing Sam68 to the cytoplasm during FMDV infection of host cells

    SciTech Connect

    Lawrence, Paul; Schafer, Elizabeth A.; Rieder, Elizabeth

    2012-03-30

    Picornavirus infection can lead to disruption of nuclear pore traffic, shut-off of cell translation machinery, and cleavage of proteins involved in cellular signal transduction and the innate response to infection. Here, we demonstrated that the FMDV 3C{sup pro} induced the cleavage of nuclear RNA-binding protein Sam68 C-terminus containing the nuclear localization sequence (NLS). Consequently, it stimulated the redistribution of Sam68 to the cytoplasm. The siRNA knockdown of Sam68 resulted in a 1000-fold reduction in viral titers, which prompted us to study the effect of Sam68 on FMDV post-entry events. Interestingly, Sam68 interacts with the internal ribosomal entry site within the 5 Prime non-translated region of the FMDV genome, and Sam68 knockdown decreased FMDV IRES-driven activity in vitro suggesting that it could modulate translation of the viral genome. The results uncover a novel role for Sam68 in the context of picornaviruses and the proteolysis of a new cellular target of the FMDV 3C{sup pro}.

  15. 78 FR 16259 - Subcommittee Meeting of the Board of Advisors to the President, Naval Postgraduate School

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-14

    ... Advisors (BOA) to the Presidents of the Naval Postgraduate School and the Naval War College (NPS). This... meeting location. For access, information, or to send written comments regarding the NPS BOA contact...

  16. The App Squad: SLJ's Advisors Weigh in on Kids' Book Apps

    ERIC Educational Resources Information Center

    Ishizuka, Kathy

    2011-01-01

    In this article, "School Library Journal's" ("SLJ") advisors talk about book apps for kids. They discuss what they like, what one should look for in discerning the best for kids and teens, and where this all might be headed.

  17. The CBT Advisor: An Expert System Program for Making Decisions about CBT.

    ERIC Educational Resources Information Center

    Kearsley, Greg

    1985-01-01

    Discusses structure, credibility, and use of the Computer Based Training (CBT) Advisor, an expert system designed to help managers make judgements about course selection, system selection, cost/benefits, development effort, and probable success of CBT projects. (MBR)

  18. The first year: Development of a LANDSAT capability at Sam Houston State University

    NASA Technical Reports Server (NTRS)

    Bounds, J. H.

    1981-01-01

    Problems encountered in initiating a LANDSAT data processing capability at Sam Houston State University are discussed. Computer requirements, financing, and academic and administrative support are addressed.

  19. What Are Faculty Advisors To Do When Their Own Career Path Does Not Satisfy Their Students?

    NASA Astrophysics Data System (ADS)

    McManus, D. A.

    2001-12-01

    As graduate students seek advice on broad career options, many faculty advisors do not know what to do. It is easy for them to do nothing. They may do nothing because they assume that their own students are interested only in an academic research career like theirs. The mistake here can be that the advisors' verbal and non-verbal communication deters students from mentioning their interests in the first place or pursuing those interests, if mentioned. Or advisors may do nothing by assuming that it is not their responsibility to advise students about career options other than being an academic researcher. The advisors' lack of knowledge about other careers may lead them to avoid the issue. The mistake here is obvious. So what are advisors to do? They can encourage students to think of their graduate study as part of their career preparation, not just a task to obtain a research degree. Creating a risk-free environment for career discussion will enable faculty advisors to learn each student's career priorities and validate exploration of broad career options. Advisors should not feel inadequate by being unable to advise about everything. No one expects them to. They can encourage their students to meet together, on their own if necessary, to discuss common career concerns, even to invite speakers, including alums, to talk about different careers and the preparation required. They can encourage their students to seek additional mentors, people more knowledgeable about careers of interest to the students. They can encourage students to take courses for career preparation, particularly courses outside of science, even though these courses "take them away from their research." And advisors should not hold students at fault if they change their minds about career paths. More information often changes minds. These are a few of the many things that advisors can do. It is essential that faculty advisors not resent students' decisions to follow a career path different from

  20. Confessions of a President's Science Advisor (George B. Pegram Lecture Series)

    SciTech Connect

    Lane, Neal

    2006-09-11

    Former science advisor to president Bill Clinton Neal Lane briefly reviews the history of the job of Science Advisor to the President and give some examples of issues he had to deal with when he was in that position, including climate change, stem cell research, the human genome, nanotechnology and research funding. He will also give his opinions about the present and future state of science in the U.S.

  1. In situ analysis of Mars soil sample with the sam gcms instrumentation onboard Curiosity : interpretation and comparison of measurements done at Rocknest and Yelloknife bay sites

    NASA Astrophysics Data System (ADS)

    Szopa, Cyril; Coll, Patrice; Cabane, Michel; Coscia, David; Buch, Arnaud; Francois, Pascaline; Millan, Maeva; Teinturier, Sammy; Navarro-Gonzales, Rafael; Glavin, Daniel; Freissinet, Caro; Steele, Andrew; Eigenbrode, Jen; Mahaffy, Paul

    2014-05-01

    The characterisation of the chemical and mineralogical composition of regolith samples collected with the Curiosity rover is a primary objective of the SAM experiment. These data should provide essential clues on the past habitability of Gale crater. Amongst the SAM suite of instruments [1], SAM-GC (Gas Chromatograph) is devoted to identify and quantify volatiles evolved from the thermal (heating up to about 900°C)/chemical (derivatization procedure) treatment of any soil sample collected by the Curiosity rover. With the aim to search for potential organic molecules outgassed from the samples, a SAM-GC analytical channel composed of thermal-desorption injector and a MXT-CLP chromatographic column was chosen to achieve all the measurements done up today, as it was designed for the separation of a wide range of volatile organic molecules. Three solid samples have been analyzed with GCMS, one sand sample collected at the Rocknest site, and two rock samples (John Klein and Cumberland respectively) collected at the Yellowknife Bay site using the Curiosity driller. All the measurements were successful and they produced complex chromatograms with both detectors used for SAM GC, i.e. a thermal conductivity detector and the SAM quandrupole mass spectrometer. Their interpretation already revealed the presence of an oxychlorine phase present in the sample which is at the origin of chlorohydrocarbons clearly identified [2] but this represents only a fraction of the GCMS signal recorded [3,4]. This work presents a systematic comparison of the GCMS measurements done for the different samples collected, supported by reference data obtained in laboratory with different spare models of the gas chromatograph, with the aim to bring new elements of interpretation of the SAM measurements. References: [1] Mahaffy, P. et al. (2012) Space Sci Rev, 170, 401-478. [2] Glavin, D. et al. (2013), JGR. [3] Leshin L. et al. (2013), Science, [4] Ming D. et al. (2013), Science, 32, 64

  2. Chemical analyses of hydroxyapatite formation on SAM surfaces modified with COOH, NH(2), CH(3), and OH functions.

    PubMed

    Hirata, Isao; Akamatsu, Mai; Fujii, Eri; Poolthong, Suchit; Okazaki, Masayuki

    2010-08-01

    Hydroxyapatite formation was examined at the surface of self-assembled monolayers (SAMs) modified with four functional groups, -COOH, -NH(2), -CH(3), and -OH. For COOH-SAM and NH(2)-SAM, scanning electron spectroscopic observation showed that flake-like sheet crystals covered the whole wafer and small broccoli-like crystals were observed occasionally on the flake-like crystal base layer. For CH(3)-SAM and OH-SAM, no flake-like sheet crystals were observed; broccoli-like crystals were observed in a dispersed manner for CH(3)-SAM, but in localized spots for OH-SAM. X-ray diffraction patterns showed a strong apatite pattern oriented toward the c-axis direction for COOH-SAM. ESCA analysis revealed distinct Ca, P, O peaks for COOH-, NH(2)-, CH(3)-, and OH-SAM. Surface plasmon resonance (SPR) analysis indicated that during the supply of supersaturated calcium phosphate solution, the deposition of precipitates increased monotonically with time for COOH-SAM, increased slightly for NH(2)-SAM, but little increase in deposition was detected for CH(3)-SAM and OH-SAM.

  3. Unanticipated coordination of tris buffer to the Radical SAM cluster of the RimO methylthiotransferase.

    PubMed

    Molle, Thibaut; Clémancey, Martin; Latour, Jean-Marc; Kathirvelu, Velavan; Sicoli, Giuseppe; Forouhar, Farhad; Mulliez, Etienne; Gambarelli, Serge; Atta, Mohamed

    2016-07-01

    Radical SAM enzymes generally contain a [4Fe-4S](2+/1+) (RS cluster) cluster bound to the protein via the three cysteines of a canonical motif CxxxCxxC. The non-cysteinyl iron is used to coordinate SAM via its amino-carboxylate moiety. The coordination-induced proximity between the cluster acting as an electron donor and the adenosyl-sulfonium bond of SAM allows for the homolytic cleavage of the latter leading to the formation of the reactive 5'-deoxyadenosyl radical used for substrate activation. Most of the structures of Radical SAM enzymes have been obtained in the presence of SAM, and therefore, little is known about the situation when SAM is not present. In this report, we show that RimO, a methylthiotransferase belonging to the radical SAM superfamily, binds a Tris molecule in the absence of SAM leading to specific spectroscopic signatures both in Mössbauer and pulsed EPR spectroscopies. These data provide a cautionary note for researchers who work with coordinative unsaturated iron sulfur clusters. PMID:27259294

  4. Sam68 Regulates S6K1 Alternative Splicing during Adipogenesis

    PubMed Central

    Song, Jingwen

    2015-01-01

    The requirement for alternative splicing during adipogenesis is poorly understood. The Sam68 RNA binding protein is a known regulator of alternative splicing, and mice deficient for Sam68 exhibit adipogenesis defects due to defective mTOR signaling. Sam68 null preadipocytes were monitored for alternative splicing imbalances in components of the mTOR signaling pathway. Herein, we report that Sam68 regulates isoform expression of the ribosomal S6 kinase gene (Rps6kb1). Sam68-deficient adipocytes express Rps6kb1-002 and its encoded p31S6K1 protein, in contrast to wild-type adipocytes that do not express this isoform. Sam68 binds an RNA sequence encoded by Rps6kb1 intron 6 and prevents serine/arginine-rich splicing factor 1 (SRSF1)-mediated alternative splicing of Rps6kb1-002, as assessed by cross-linking and immunoprecipitation (CLIP) and minigene assays. Depletion of p31S6K1 with small interfering RNAs (siRNAs) partially restored adipogenesis of Sam68-deficient preadipocytes. The ectopic expression of p31S6K1 in wild-type 3T3-L1 cells resulted in adipogenesis differentiation defects, showing that p31S6K1 is an inhibitor of adipogenesis. Our findings indicate that Sam68 is required to prevent the expression of p31S6K1 in adipocytes for adipogenesis to occur. PMID:25776557

  5. Overview of SAM results obtained at Gale Crater during the 180 first sols

    NASA Astrophysics Data System (ADS)

    Coll, P.; Mahaffy, P. R.; Cabane, M.; Webster, C. R.; Archer, D.; Atreya, S. K.; Benna, M.; Brinckerhoff, W. B.; Brunner, A.; Buch, A.; Conrad, P.; Coscia, D.; Dobson, N.; Dworkin, J.; Eigenbrode, J.; Farley, K.; Flesch, G.; Franz, H.; Freissinet, C.; Galvin, D.; Gorevan, S.; Harpold, D.; Hengemihle, J.; Jaeger, F.; Johnson, C.; Johnson, M.; Jones, J.; Lefavor, M.; Leshin, L.; Lyness, E.; Malespin, C.; Manning, H.; Martin, D.; McAdam, A.; McKay, C.; Miller, K.; Ming, D. W.; Morris, R. V.; Navarro-González, R.; Niles, P.; Nolan, T.; Owen, T.; Pavolv, A.; Prats, B.; Pepin, R.; Raaen, E.; Raulin, F.; Steele, A.; Stern, J.; Squyres, S.; Sutter, B.; Summons, R. E.; Szopa, C.; Tan, F.; Teinturier, S.; Trainer, M.; Wong, M.; Wray, J.

    2013-09-01

    During the first 180 sols of Curiosity's landed mission on Mars (8/6/2012 to 2/7/2013) SAM sampled the atmosphere more than a dozen times, the dusty sandpile named Rocknest and a basin site named John Klein on the floor of Gale crater. The atmospheric experiments utilized SAM's quadrupole mass spectrometer (QMS) and its tunable laser spectrometer (TLS) while the solid sample experiments also utilized the gas chromatograph (GC). Although a number of core experiments were pre-programmed and stored in SAM EEProm, the high level SAM scripting language enabled the team to often optimize experiments based on prior runs. SAM and its Experiment Sequences exercised during the First 120 Sols: The SAM instruments, its gas processing system (GPS) and its sample manipulation system (SMS) have been already described [1]. During the first few weeks of the landed mission SAM carried out a variety of instrument health checks and then began a series of atmospheric experiments to measure atmospheric composition and isotope ratios. From sol 56 to 102 Curiosity lingered at Rocknest to clean out the surfaces of the sample processing system by scooping several times into this fine grained material, vibrating to abrade possible contamination from surfaces, and then discarding before delivery of sample to SAM from the 5th scoop.

  6. Winning Attitude & Dedication to Physical Therapy Keep Sam Schmidt on Track

    ERIC Educational Resources Information Center

    Bosley, Nikki Prevenslik

    2006-01-01

    This article relates how Sam Schmidt returned to living a productive life after an accident left him with spinal cord injury. Schmidt was a former Indy Racing League driver who founded Sam Schmidt Motorsports after his accident in 2000. Schmidt's car hit the wall as he exited turn two during a practice session at Walt Disney World Speedway in…

  7. NaCo/SAM observations of sources at the Galactic Center

    NASA Astrophysics Data System (ADS)

    Sanchez-Bermudez, J.; Schödel, R.; Alberdi, A.; Pott, J. U.

    2012-07-01

    Sparse aperture masking (SAM) interferometry combined with Adaptive Optics (AO) is a technique that is uniquely suited to investigate structures near the diffraction limit of large telescopes. The strengths of the technique are a robust calibration of the Point Spread Function (PSF) while maintaining a relatively high dynamic range. We used SAM+AO observations to investigate the circumstellar environment of several bright sources with infrared excess in the central parsec of the Galaxy. For our observations, unstable atmospheric conditions as well as significant residuals after the background subtraction presented serious problems for the standard approach of calibrating SAM data via interspersed observations of reference stars. We circumvented these difficulties by constructing a synthesized calibrator directly from sources within the field-of-view. When observing crowded fields, this novel method can boost the efficiency of SAM observations because it renders interspersed calibrator observations unnecessary. Here, we presented the first NaCo/SAM images reconstructed using this method.

  8. Radical SAM catalysis via an organometallic intermediate with an Fe-[5'-C]-deoxyadenosyl bond.

    PubMed

    Horitani, Masaki; Shisler, Krista; Broderick, William E; Hutcheson, Rachel U; Duschene, Kaitlin S; Marts, Amy R; Hoffman, Brian M; Broderick, Joan B

    2016-05-13

    Radical S-adenosylmethionine (SAM) enzymes use a [4Fe-4S] cluster to cleave SAM to initiate diverse radical reactions. These reactions are thought to involve the 5'-deoxyadenosyl radical intermediate, which has not yet been detected. We used rapid freeze-quenching to trap a catalytically competent intermediate in the reaction catalyzed by the radical SAM enzyme pyruvate formate-lyase activating enzyme. Characterization of the intermediate by electron paramagnetic resonance and (13)C, (57)Fe electron nuclear double-resonance spectroscopies reveals that it contains an organometallic center in which the 5' carbon of a SAM-derived deoxyadenosyl moiety forms a bond with the unique iron site of the [4Fe-4S] cluster. Discovery of this intermediate extends the list of enzymatic bioorganometallic centers to the radical SAM enzymes, the largest enzyme superfamily known, and reveals intriguing parallels to B12 radical enzymes.

  9. S.A.M., the Italian Martian simulation chamber.

    PubMed

    Galletta, G; Ferri, F; Fanti, G; D'Alessandro, M; Bertoloni, G; Pavarin, D; Bettanini, C; Cozza, P; Pretto, P; Bianchini, G; Debei, S

    2006-12-01

    The Martian Environment Simulator (SAM "Simulatore di Ambiente Marziano") is a interdisciplinary project of Astrobiology done at University of Padua. The research is aimed to the study of the survival of the microorganisms exposed to the "extreme" planetary environment. The facility has been designed in order to simulate Mars' environmental conditions in terms of atmospheric pressure, temperature cycles and UV radiation dose. The bacterial cells, contained into dedicated capsules, will be exposed to thermal cycles simulating diurnal and seasonal Martian cycles. The metabolism of the different biological samples will be analysed at different phases of the experiment, to study their survival and eventual activity of protein synthesis (mortality, mutations and capability of DNA repairing). We describe the experimental facility and provide the perspectives of the biological experiments we will perform in order to provide hints on the possibility of life on Mars either autochthonous or imported from Earth.

  10. Radical SAM-Mediated Methylation of Ribosomal RNA

    PubMed Central

    Stojkovic, Vanja; Fujimori, Danica Galonić

    2015-01-01

    Post-transcriptional modifications of RNA play an important role in a wide range of biological processes. In ribosomal RNA (rRNA), methylation of nucleotide bases is the predominant modification. In recent years, methylation of adenosine 2503 (A2503) in bacterial 23S rRNA has attracted significant attention due to both the unusual regioselectivity of the methyl group incorporation, as well as the pathophysiological roles of the resultant methylations. Specifically, A2503 is methylated at the C2 and C8 positions of the adenine ring, and the introduced modifications have a profound impact on translational fidelity and antibiotic resistance, respectively. These modifications are performed by RlmN and Cfr, two members, of the recently discovered class of radical S-adenosylmethionine (radical SAM) methylsynthases. Here, we present several methods that can be used to evaluate the activity of these enzymes, under both in vivo and in vitro conditions. PMID:26253978

  11. S.A.M., the Italian Martian Simulation Chamber

    NASA Astrophysics Data System (ADS)

    Galletta, G.; Ferri, F.; Fanti, G.; D'Alessandro, M.; Bertoloni, G.; Pavarin, D.; Bettanini, C.; Cozza, P.; Pretto, P.; Bianchini, G.; Debei, S.

    2006-12-01

    The Martian Environment Simulator (SAM “Simulatore di Ambiente Marziano”) is a interdisciplinary project of Astrobiology done at University of Padua. The research is aimed to the study of the survival of the microorganisms exposed to the “extreme” planetary environment. The facility has been designed in order to simulate Mars’ environmental conditions in terms of atmospheric pressure, temperature cycles and UV radiation dose. The bacterial cells, contained into dedicated capsules, will be exposed to thermal cycles simulating diurnal and seasonal Martian cycles. The metabolism of the different biological samples will be analysed at different phases of the experiment, to study their survival and eventual activity of protein synthesis (mortality, mutations and capability of DNA reparing). We describe the experimental facility and provide the perspectives of the biological experiments we will perform in order to provide hints on the possibility of life on Mars either autochthonous or imported from Earth.

  12. Presentation on a Space Acceleration Measurement System (SAMS)

    NASA Technical Reports Server (NTRS)

    Chase, Theodore L.

    1990-01-01

    The primary objective of the Space Acceleration Measurement Systems (SAMS) project is to provide an acceleration measurement system capable of serving a wide variety of space experiments. The design of the system being developed under this project takes into consideration requirements for experiments located in the middeck, in the orbiter bay, and in Spacelab. In addition to measuring, conditioning, and recording accelerations, the system will be capable of performing complex calculations and interactive control. The main components consist of a remote triaxial optical storage device. In operation, the triaxial sensor head produces output signals in response to acceleration inputs. These signals are preamplified, filtered and converted into digital data which is then transferred to optical memory. The system design is modular, facilitating both software and hardware upgrading as technology advances. Two complete acceleration measurement flight systems will be build and tested under this project.

  13. Social activity method (SAM): A fractal language for mathematics

    NASA Astrophysics Data System (ADS)

    Dowling, Paul

    2013-09-01

    In this paper I shall present and develop my organisational language, social activity method (SAM), and illustrate some of its applications. I shall introduce a new scheme for modes of recontextualisation that enables the analysis of the ways in which one activity - which might be school mathematics or social research or any empirically observed regularity of practice - recontextualises the practice of another and I shall also present, deploy, and develop an existing scheme - domains of action - in an analysis of school mathematics examination papers and in the structuring of what I refer to as the esoteric domain. This domain is here conceived as a hybrid domain of, first, linguistic and extralinguistic resources that are unambiguously mathematical in terms of both expression and content and, second, pedagogic theory - often tacit - that enables the mathematical gaze onto other practices and so recontextualises them. A second and more general theme that runs through the paper is the claim that there is nothing that is beyond semiosis, that there is nothing to which we have direct access, unmediated by interpretation. This state of affairs has implications for mathematics education. Specifically, insofar as an individual's mathematical semiotic system is under continuous development - the curriculum never being graspable all at once - understanding - as a stable semiotic moment - of any aspect or object of mathematics is always localised to the individual and is at best transient, and the sequencing of such moments may well also be more individualised than consistent in some correspondence with the sequencing of the curriculum. This being the case, a concentration on understanding as a goal may well serve to inhibit the pragmatic acquisition and deployment of mathematical technologies, which should be the principal aim of mathematics teaching and learning. The paper is primarily concerned with mathematics education. SAM, however, is a language that is available for

  14. Sam68 is Overexpressed in Epithelial Ovarian Cancer and Promotes Tumor Cell Proliferation.

    PubMed

    Dong, Lijuan; Che, Hailuo; Li, Mingmei; Li, Xuepeng

    2016-01-01

    BACKGROUND Epithelial ovarian cancer (EOC) is the deadliest gynecological malignancy, and evidence is accumulating on how molecular markers may be associated with the origin and process of EOC. Sam68 (Src-associated in mitosis, of 68 kD), is a K homology domain RNA-binding protein that has been investigated as a risk factor in multiple types of tumors. The aim of the present study was to investigate the contribution of the Sam68 gene in the pathogenesis of EOC. MATERIAL AND METHODS Western blot assay and real-time quantitative PCR methods were performed to examine Sam68 expression in EOC tissue specimens. The association of Sam68 expression with clinic-pathologic variables of EOC was evaluated. Then gain-of-function and loss-of-function strategies were adopted to examine the regulation of Sam68 on the proliferation of EOC OVCAR-3 cells using CCK-8 and colony forming assays. RESULTS Sam68 was overexpressed in both mRNA and protein levels in EOC tumor tissue (n=152) in an association with malignant factors of EOC such as International Federation of Gynecology and Obstetrics (FIGO) stage, residual tumor size (cm), histological grade, and lymph node metastasis. In vitro results demonstrated that Sam68 overexpression was upregulated while Sam68 knockdown downregulated the proliferation of EOC OVCAR-3 cells via regulation of cell growth and colony formation. CONCLUSIONS Sam68 was overexpressed in EOC tissue in association with such cancer malignant factors of FIGO stage, histological grade, and lymph node metastasis, and also positively regulated the proliferation of EOC cells. Our research suggests that Sam68 might accelerate cell cycle progression, and present as a prognostic marker for EOC. PMID:27623016

  15. Sam68 is Overexpressed in Epithelial Ovarian Cancer and Promotes Tumor Cell Proliferation

    PubMed Central

    Dong, Lijuan; Che, Hailuo; Li, Mingmei; Li, Xuepeng

    2016-01-01

    Background Epithelial ovarian cancer (EOC) is the deadliest gynecological malignancy, and evidence is accumulating on how molecular markers may be associated with the origin and process of EOC. Sam68 (Src-associated in mitosis, of 68 kD), is a K homology domain RNA-binding protein that has been investigated as a risk factor in multiple types of tumors. The aim of the present study was to investigate the contribution of the Sam68 gene in the pathogenesis of EOC. Material/Methods Western blot assay and real-time quantitative PCR methods were performed to examine Sam68 expression in EOC tissue specimens. The association of Sam68 expression with clinic-pathologic variables of EOC was evaluated. Then gain-of-function and loss-of-function strategies were adopted to examine the regulation of Sam68 on the proliferation of EOC OVCAR-3 cells using CCK-8 and colony forming assays. Results Sam68 was overexpressed in both mRNA and protein levels in EOC tumor tissue (n=152) in an association with malignant factors of EOC such as International Federation of Gynecology and Obstetrics (FIGO) stage, residual tumor size (cm), histological grade, and lymph node metastasis. In vitro results demonstrated that Sam68 overexpression was upregulated while Sam68 knockdown downregulated the proliferation of EOC OVCAR-3 cells via regulation of cell growth and colony formation. Conclusions Sam68 was overexpressed in EOC tissue in association with such cancer malignant factors of FIGO stage, histological grade, and lymph node metastasis, and also positively regulated the proliferation of EOC cells. Our research suggests that Sam68 might accelerate cell cycle progression, and present as a prognostic marker for EOC. PMID:27623016

  16. The key role of the Barefoot Fisheries Advisors in the co-managed TURF system of Galicia (NW Spain).

    PubMed

    Macho, Gonzalo; Naya, Inés; Freire, Juan; Villasante, Sebastián; Molares, José

    2013-12-01

    Many authors have pointed out the need for simpler assessment and management procedures for avoiding overexploitation in small-scale fisheries. Nevertheless, models for providing scientific advice for sustainable small-scale fisheries management have not yet been published. Here we present one model; the case of the Barefoot Fisheries Advisors (BFAs) in the Galician co-managed Territorial Users Rights for Fishing. Based on informal interviews, gray literature and our personal experience by being involved in this process, we have analyzed the historical development and evolution of roles of this novel and stimulating actor in small-scale fisheries management. The Galician BFA model allows the provision of good quality and organized fisheries data to facilitate and support decision-making processes. The BFAs also build robust social capital by acting as knowledge collectors and translators between fishers, managers, and scientists. The BFAs have become key actors in the small-scale fisheries management of Galicia and a case for learning lessons.

  17. Roles of religious and spiritual advisors among adults in Singapore with mental illnesses.

    PubMed

    Picco, Louisa; Subramaniam, Mythily; Abdin, Edimansyah; Vaingankar, Janhavi Ajit; Zhang, Yunjue; Chong, Siow Ann

    2013-11-01

    OBJECTIVE Information is limited concerning the role of religious and spiritual advisors in providing help to people with mental illnesses in Singapore. This study examined that role, as well as the satisfaction with and the perceived effectiveness of the services provided, among people with mental health problems. METHODS Data were collected as part of a nationally representative household survey of residents 18 years and older in Singapore. The Composite International Diagnostic Interview, version 3.0, was used to diagnose mental illness as well as to collect information about the mental health services respondents had sought. RESULTS A total of 6,616 respondents completed the survey; in the overall sample, 1.5% reported seeking help from religious or spiritual advisors. This rate increased to 6.6% among those with at least one mental illness, with the prevalence being higher among respondents with lifetime dysthymia, generalized anxiety disorder, or bipolar disorder. Sociodemographic correlates associated with a lower likelihood of consultation with a religious or spiritual advisor included reporting "other" race-ethnicity as well as faith in Buddhism, Hinduism, or Islam. Most respondents who sought help from a religious or spiritual advisor in the last 12 months were satisfied with the help they received, and about half reported it to be very useful. CONCLUSIONS Religious and spiritual advisors are an important source of help for people with mental illness, and a majority of respondents with a mental illness were satisfied with the support they received from these sources. PMID:23903293

  18. Guide to good practices for the selection, training, and qualification of shift technical advisors

    SciTech Connect

    1993-02-01

    The DOE Guide to Good Practices For the Selection, Training, and Qualification of Shift Technical Advisors can be used by any DOE nuclear facility that has implemented the shift technical advisor (STA) position. DOE Order 5480.20A, Personnel Selection, Qualification and Training Requirements for DOE Nuclear Facilities, states that only Category A reactors must have a shift technical advisor. However, many DOE nuclear facilities have implemented the shift technical advisor position to provide independent on-shift technical advice and counsel to the shift operating personnel to help determine cause and mitigation of facility accidents. Those DOE nuclear facilities that have implemented or are going to implement the shift technical advisor position will find this guide useful. This guide addresses areas that may be covered by other training programs. In these cases, it is unnecessary (and undesirable) to duplicate these areas in the STA training program as long as the specific skills and knowledge essential for STAs are addressed. The guide is based on the premise that the trainee has not completed any facility-specific training other than general employee training.

  19. MSL/SAM Measurements of Volatile Isotopes, and their Implications for Atmospheric Loss

    NASA Astrophysics Data System (ADS)

    Atreya, Sushil K.; Mahaffy, Paul; Webster, Christopher; Wong, Michael; Conrad, Pamela; Franz, Heather; Grotzinger, John; Jones, John; Leshin, Laurie; Malespin, Charles; Manning, Heidi; Navarro-Gonzalez, Raphael; Owen, Tobias; Pepin, Robert; Schwenzer, Susanne; Trainer, Melissa

    2014-05-01

    High precision measurements of the isotopes of carbon and oxygen in CO2, hydrogen in H2O, nitrogen in N2, and argon in the martian atmosphere have been made by the Sample Analysis at Mars (SAM) instrument on the Curiosity Rover [1,2,3,4]. The resulting values in per mil are 46 for δ13C (relative to VPDB reference standard), 48 for δ18O (VSMOW), 5880 for δD (VSMOW), 572 for δ15N (relative to earth atmosphere), and 4.2 for 36Ar/38Ar (or δ38Ar=310 relative to sun reference standard). The observed enrichment of the heavier isotope over the lighter isotope means that loss to space rather than loss to the surface dominates the isotopic composition in the martian atmosphere. Vertical mixing transports the volatiles from the surface up to the upper atmosphere. While eddy diffusion and molecular diffusion control the distribution of the noble gases, photochemistry also plays a significant role in the distribution of the other volatiles as they diffuse to the upper atmosphere. The above SAM data on the isotopic ratios of carbon, oxygen, hydrogen, nitrogen and argon implies a massive loss of the atmosphere from Mars in the past four billion years. Only hydrogen (hence water) is likely to escape thermally due to the low exospheric temperature of Mars. However, the lack of intrinsic magnetic field on Mars allows solar wind to interact directly with the atmosphere, thus opening up a myriad of possibilities for escape of volatiles from Mars. One such mechanism studied by the ion mass analyzer instrument on Mars Express finds that at current rate of erosion by solar wind, Mars may have lost between 0.2 and 4 millibar of the CO2 atmosphere in the past 3.5 billion years [5]. However, these authors [5] stress that other mechanisms including photochemical, sputtering and cold plasma escape may result in up to 1000 times greater rate of atmospheric loss based on models. Any fractionation in the isotopes of the heavy noble gas, xenon, would have occurred prior to approximately 4 Ga

  20. The nuclear protein Sam68 is recruited to the cytoplasmic stress granules during enterovirus 71 infection.

    PubMed

    Zhang, Hua; Chen, Ning; Li, Pengfei; Pan, Ziye; Ding, Yun; Zou, Dehua; Li, Liyang; Xiao, Lijie; Shen, Binglei; Liu, Shuxia; Cao, Hongwei; Cui, Yudong

    2016-07-01

    Our previous study found that the nuclear protein, 68-kDa Src-associated in mitosis protein (Sam68), is translocated to the cytoplasm and forms punctate pattern during enterovirus 71 (EV71) infection [Virus Research, 180 (2014), 1-11]. However, the exact function of this punctate pattern in cytoplasm during EV71 infection remains unknown. In this study, we firstly have examined this punctate pattern of Sam68 re-localization in the cytoplasm, and observed the obvious recruitments of Sam68 to the EV71-induced stress granules (SGs). Sam68, belongs to the KH domain family of RNA binding proteins (RBPs), was then confirmed that its KH domain was essential for this recruitment. Nevertheless, Knockdown of Sam68 expression using ShRNA had no effects on SGs assembly, indicating that Sam68 is not a constitutive component of the SGs during EV71 infection. Lastly, we investigated the importance of microtubulin transport to SGs aggregation, and revealed that microtubule depolymerization inhibited SGs formation, suggesting that EV71-induced SGs move throughout the cytoplasm in a microtubule-dependent manner. Taken together, these results illuminated that EV71 infections can induce SGs formation, and Sam68, as a SGs component, migrates alone with SGs dependent on intact microtubule upon the viral infections. These findings may provide novel underlying mechanism for delineating the role of SGs during EV71 infection. PMID:27057671

  1. The integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens is related to temperate bacteriophages.

    PubMed

    Boccard, F; Smokvina, T; Pernodet, J L; Friedmann, A; Guérineau, M

    1989-03-01

    Streptomyces ambofaciens ATCC23877 and derivatives contain the 11-kb element pSAM2 present in an integrated state or as a free and integrated plasmid. This element, able to integrate site-specifically in the genome of different Streptomyces species, is conjugative and mobilizes chromosomal markers. Besides these plasmid functions, we have shown that the site-specific recombination system of pSAM2 presents strong similarities with that of several temperate phages. The integration event is promoted by a site-specific recombinase of the integrase family. The int gene encoding this integrase is closely linked to the plasmid attachment site (attP). A small open reading frame (ORF) overlaps the int gene and the predicted protein exhibits similarities with Xis proteins involved in phages excision. The integrated copy of pSAM2 in strain ATCC23877 is flanked by att sequences (attL and attR). Another att sequence (attX) is present in this strain and attX and attL are the boundaries of a 42-kb fragment (xSAM1) absent, as well as pSAM2, from S.ambofaciens DSM40697. Sequences partially similar to pSAM2 int gene are found near the chromosomal integration zone in both S.ambofaciens strains. The possible origin of pSAM2, an element carrying plasmid as well as phage features, is discussed.

  2. Nothing to declare: mandatory and voluntary disclosure leads advisors to avoid conflicts of interest.

    PubMed

    Sah, Sunita; Loewenstein, George

    2014-02-01

    Professionals face conflicts of interest when they have a personal interest in giving biased advice. Mandatory disclosure--informing consumers of the conflict--is a widely adopted strategy in numerous professions, such as medicine, finance, and accounting. Prior research has shown, however, that such disclosures have little impact on consumer behavior, and can backfire by leading advisors to give even more biased advice. We present results from three experiments with real monetary stakes. These results show that, although disclosure has generally been found to be ineffective for dealing with unavoidable conflicts of interest, it can be beneficial when providers have the ability to avoid conflicts. Mandatory and voluntary disclosure can deter advisors from accepting conflicts of interest so that they have nothing to disclose except the absence of conflicts. We propose that people are averse to being viewed as biased, and that policies designed to activate reputational and ethical concerns will motivate advisors to avoid conflicts of interest. PMID:24379156

  3. Sam68 Mediates the Activation of Insulin and Leptin Signalling in Breast Cancer Cells

    PubMed Central

    Pérez-Pérez, Antonio; Sánchez-Jiménez, Flora; Vilariño-García, Teresa; de la Cruz, Luis; Virizuela, Juan A.; Sánchez-Margalet, Víctor

    2016-01-01

    Obesity is a well-known risk factor for breast cancer development in postmenopausal women. High insulin and leptin levels seem to have a role modulating the growth of these tumours. Sam68 is an RNA-binding protein with signalling functions that has been found to be overexpressed in breast cancer. Moreover, Sam68 may be recruited to insulin and leptin signalling pathways, mediating its effects on survival, growth and proliferation in different cellular types. We aimed to study the expression of Sam68 and its phosphorylation level upon insulin and leptin stimulation, and the role of Sam68 in the proliferative effect and signalling pathways that are activated by insulin or leptin in human breast adenocarcinoma cells. In the human breast adenocarcinoma cell lines MCF7, MDA-MB-231 and BT-474, Sam68 protein quantity and gene expression were increased upon leptin or insulin stimulation, as it was checked by qPCR and immunoblot. Moreover, both insulin and leptin stimulation promoted an increase in Sam68 tyrosine phosphorylation and negatively regulated its RNA binding capacity. siRNA was used to downregulate Sam68 expression, which resulted in lower proliferative effects of both insulin and leptin, as well as a lower activation of MAPK and PI3K pathways promoted by both hormones. These effects may be partly explained by the decrease in IRS-1 expression by down-regulation of Sam68. These results suggest the participation of Sam68 in both leptin and insulin receptor signaling in human breast cancer cells, mediating the trophic effects of these hormones in proliferation and cellular growth. PMID:27415018

  4. Denitrification of the polar winter stratosphere - Implications of SAM II cloud formation temperatures

    NASA Technical Reports Server (NTRS)

    Hamill, Patrick; Toon, O. B.

    1990-01-01

    The SAM II extinction profiles and the associated temperature profiles are used to determine the amount of denitrification of the winter polar stratospheres. Clear evidence of the denitrification process in the Antarctic data is seen. There are indications in the Arctic data that denitrification mechanisms may be at work there also. At the latitudes observed by the SAM II satellite system, denitrification begins before the formation of extensive ice clouds and may be due to sedimentation of nitric acid particles. However, the possibility of dinitrification by type II PSCs at latitudes not observed by SAM II cannot be excluded.

  5. Mixed-SAM surfaces monitoring CTX-protein part I: Using atomic force microscope measurements.

    PubMed

    Chang, Joe-Ming; Tseng, Fan-Gang; Chieng, Ching-Chang

    2010-12-01

    Fast and efficient detection of Cobra cardiotoxin (CTX) protein molecules on biochip surfaces is an example of application in biotechnology. One potential application of mixed self assembled monolayers (SAMs) as chip surfaces yield different binding affinities of the CTX proteins, a series of studies on the interaction force between CTX proteins and the mixed SAMs surfaces formed from mixtures of two thiols with the same/different chain lengths and/or with the same/different terminal groups will be investigated. In these dual papers, the mixed SAMs of n-alkinethiol SAMs of different chain lengths are chosen as the first examples of this series due to the simple functions of the mixed SAMs surface structure. Thus, the adhesion force measurements of CTX protein molecules on mixed SAMs of n-alkinethiol SAMs of different chain lengths: 1-decanethiol (C9) and 1-hexanethiol (C5) with different mixing ratios are developed and conducted using atomic force microscope (AFM). There are two major tasks in Part I of the dual papers: the development of the AFM measurements providing reliable information, and selection of the surface with highest binding affinity among this mixed SAMs group. Results indicate that the adhesion forces for CTX protein molecules on mixed SAMs with mixing ratio (χ(C9)) of 0.25, 0.5, 0.75 and 1, are 1.26, 1.8, 1.38, and 1.25 folds respectively, compared with the adhesion force of CTX protein molecules on the C5 surface only. Therefore, the SAM surfaces of χ(C9) = 0.5 is the best choice as a biomaterial sensor of this group of mixed SAMs because the strongest binding force and highest efficiency. Effects of the loading force of the AFM operation, the radius of curvature of the AFM tip, and the AFM tip endurance as well as control experiments were examined to ensure the quantitative determination of adhesion force for AFM measurement. The physical mechanism of protein adsorption on SAM surfaces will be studied and analyzed by molecular dynamics (MD

  6. A comparative study of aerosol extinction measurements made by the SAM II and SAGE satellite experiments

    NASA Technical Reports Server (NTRS)

    Yue, G. K.; Mccormick, M. P.; Chu, W. P.

    1984-01-01

    SAM II and SAGE are two satellite experiments designed to measure stratospheric aerosol extinction using the technique of solar occultation or limb extinction. Although each sensor is mounted aboard a different satellite, there are occasions when their measurement locations are nearly coincident, thereby providing opportunities for a measurement comparison. In this paper, the aerosol extinction profiles and daily contour plots for some of these events in 1979 are reported. The comparisons shown in this paper demonstrate that SAM II and SAGE are producing similar aerosol extinction profiles within their measurement errors and that since SAM II has been previously validated, these results show the validity of the SAGE aerosol measurements.

  7. Profile of a leader: pioneer government advisor: Laura Holland, RN, RRC, CBE, LLD (1883 - 1956).

    PubMed

    Paulson, E; Zilm, G; Warbinek, E

    2000-01-01

    Laura Holland, nurse and social worker, became one of Canada's first official "nurse advisors" to government when she was appointed Advisor to the B.C. Ministry of Health and Welfare from 1938 to 1945. Before that appointment, she had served, with distinction, as a Nursing Sister in World War I, brought innovative Red Cross and public health advances to northern Ontario, reformed Children's Aid services in Vancouver and B.C., and started a new department that combined nursing/social work in the field during the Great Depression of the 1930s. She was an extraordinary leader.

  8. 40 CFR 35.4195 - Are there certain people my group cannot select to be our technical advisor, grant administrator...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the contractor; (b) In the case of a technical advisor, a person or entity doing work for the Federal... technical advisor; and (c) Any person who is on the List of Parties Excluded from Federal Procurement or NonProcurement Programs....

  9. 17 CFR 4.41 - Advertising by commodity pool operators, commodity trading advisors, and the principals thereof.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 17 Commodity and Securities Exchanges 1 2013-04-01 2013-04-01 false Advertising by commodity pool... Securities Exchanges COMMODITY FUTURES TRADING COMMISSION COMMODITY POOL OPERATORS AND COMMODITY TRADING ADVISORS Advertising § 4.41 Advertising by commodity pool operators, commodity trading advisors, and...

  10. College Advisor, Student, and Senior Staff Member Perceptions of Academic Advising Modalities and Types: A Community College Case Study

    ERIC Educational Resources Information Center

    Marvin, Daniel C.

    2013-01-01

    Institutions of higher learning, their senior staff members, and advisors are always striving to create advising modalities and utilize matching advising types that work best for the individual student. This qualitative single-case study was conducted to examine perceptions of the students, advisors, and senior staff members of the advising…

  11. 76 FR 33376 - Altegris Advisors, L.L.C. and Northern Lights Fund Trust; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-08

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION Altegris Advisors, L.L.C. and Northern Lights Fund Trust; Notice of Application June 1, 2011...: Altegris Advisors, L.L.C. (the ``Adviser'') and Northern Lights Fund Trust (the ``Trust''). DATES:...

  12. Contextualizing Gay-straight Alliances: Student, Advisor, and Structural Factors Related to Positive Youth Development among Members

    ERIC Educational Resources Information Center

    Poteat, V. Paul; Yoshikawa, Hirokazu; Calzo, Jerel P.; Gray, Mary L.; DiGiovanni, Craig D.; Lipkin, Arthur; Mundy-Shephard, Adrienne; Perrotti, Jeff; Scheer, Jillian R.; Shaw, Matthew P.

    2015-01-01

    Gay-straight alliances (GSAs) may promote resilience. Yet, what GSA components predict well-being? Among 146 youth and advisors in 13 GSAs (58% lesbian, gay, bisexual, or questioning; 64% White; 38% received free/reduced-cost lunch), student (demographics, victimization, attendance frequency, leadership, support, control), advisor (years served,…

  13. The Role of the Graduate-Level Academic Advisor for Military and Student Veterans: An Ethnographic Study

    ERIC Educational Resources Information Center

    Miller, Michelle A.

    2013-01-01

    The role of a graduate-level academic advisor is essential to all levels of higher education. With the introduction of the Post 9/11 GI Bill, there has been an influx of military and student veterans enrolling in postsecondary and graduate-level education programs. The role of the academic advisor has increased significantly with the influx of…

  14. How Do Black Male College Students Develop Supportive Relationships with White Faculty Members and Advisors at a Predominately White Institution

    ERIC Educational Resources Information Center

    Franco, Karina Elizabeth

    2012-01-01

    This qualitative study examined how Black male students develop supportive relationships with White faculty members and advisors at a predominately White urban institution in the Southeastern United State. A multiple-case study method was used to explore how Black males define support, what faculty and advisor characteristics attract Black male…

  15. The Impact of Education Support Advisors on the Attendance, Discipline, and Achievement of At-Risk Students

    ERIC Educational Resources Information Center

    Townsend, Cathy J.

    2010-01-01

    This quantitative, causal-comparative study utilized ex post facto data to examine the impact of education support advisors (ESAs) on the discipline, attendance, and achievement of at-risk students in Grades 3-12. Fourteen education support advisors working in 16 elementary and 8 secondary schools of this mid-Atlantic school district consistently…

  16. The Relationship of Worldviews of Advisors and Students and Satisfaction with Advising: A Case of Homogenous Group Impact

    ERIC Educational Resources Information Center

    Coll, Jose E.; Zalaquett, Carlos

    2008-01-01

    The purpose of this study was to investigate whether differences in world-views between academic advisors and their advisees (both traditional and non-traditional students) impact students' use of and satisfaction with the advising process. This study surveyed 115 students and 5 advisors from a four-year liberal arts university in southeastern…

  17. A Matter of Alignment: An Organizational Analysis of the Advisor Role in Three Small Urban High Schools

    ERIC Educational Resources Information Center

    Phillippo, Katherine L.

    2009-01-01

    Recent research literature suggests that students benefit from positive relationships with their teachers. Small high schools attempt to formalize expectations for such relationships through a variety of organizational structures, including the advisor role. As advisors, teachers work with a group of students in order to guide and support them.…

  18. Large scale study on the variation of RF energy absorption in the head & brain regions of adults and children and evaluation of the SAM phantom conservativeness

    NASA Astrophysics Data System (ADS)

    Keshvari, J.; Kivento, M.; Christ, A.; Bit-Babik, G.

    2016-04-01

    This paper presents the results of two computational large scale studies using highly realistic exposure scenarios, MRI based human head and hand models, and two mobile phone models. The objectives are (i) to study the relevance of age when people are exposed to RF by comparing adult and child heads and (ii) to analyze and discuss the conservativeness of the SAM phantom for all age groups. Representative use conditions were simulated using detailed CAD models of two mobile phones operating between 900 MHz and 1950 MHz including configurations with the hand holding the phone, which were not considered in most previous studies. The peak spatial-average specific absorption rate (psSAR) in the head and the pinna tissues is assessed using anatomically accurate head and hand models. The first of the two mentioned studies involved nine head-, four hand- and two phone-models, the second study included six head-, four hand- and three simplified phone-models (over 400 configurations in total). In addition, both studies also evaluated the exposure using the SAM phantom. Results show no systematic differences between psSAR induced in the adult and child heads. The exposure level and its variation for different age groups may be different for particular phones, but no correlation between psSAR and model age was found. The psSAR from all exposure conditions was compared to the corresponding configurations using SAM, which was found to be conservative in the large majority of cases.

  19. Random Walk Model for the Growth of Monolayer in Dip Pen Nanolithography

    NASA Astrophysics Data System (ADS)

    Kim, H.; Ha, S.; Jang, J.

    2013-02-01

    By using a simple random-walk model, we simulate the growth of a self-assembled monolayer (SAM) pattern generated in dip pen nanolithography (DPN). In this model, the SAM pattern grows mainly via the serial pushing of molecules deposited from the tip. We examine various SAM patterns, such as lines, crosses, and letters by changing the tip scan speed.

  20. Working Together to Deliver Usable Climate Information to Agricultural Producers and Advisors in the U.S. Corn Belt

    NASA Astrophysics Data System (ADS)

    Prokopy, L. S.; Widhalm, M.

    2012-12-01

    Weather and climate patterns are a driving force behind the success or failure of cropping systems. With U.S. corn and soybean production accounting for nearly one-third of global supplies and contributing $100 billion annually to the national economy, the ability to successfully produce crops under more variable climate conditions is critical. Useful to Usable (U2U): Transforming Climate Variability and Change Information for Cereal Crop Producers is a USDA-funded research and extension project focused on delivering to producers and farm advisors the resources and training they need to more effectively manage variable climate conditions. The U2U team is a diverse and uniquely qualified group of climatologists, crop modelers, agronomists, economists, and social scientists from 10 Midwestern universities and two NOAA Regional Climate Centers. Together, we strive to help producers make better long-term plans on what, when and where to plant and also how to manage crops for maximum yields and minimum environmental damage. Under development are tools that will allow the agricultural community to examine the financial, production, and environmental outcomes of different management options and climate scenarios so farmers can choose strategies that fit their capabilities and acceptable levels of risk. Researchers are currently using existing data and agro-climate models to investigate the impact of climate conditions on key topics such as crop yields, fieldwork opportunities, nitrogen management, and the cost-effectiveness of irrigation and tiling. To ensure relevance and usability of U2U products, our social science team is using a number of techniques including surveys and focus groups to integrate stakeholder interests, needs, and concerns into all aspects of U2U research. It is through this coupling of physical and social science disciplines that we strive to transform existing climate information into actionable knowledge. This presentation will elaborate on U2U

  1. Sam Goudsmit--His Physics and His Statesmanship

    NASA Astrophysics Data System (ADS)

    Bederson, Benjamin

    2010-03-01

    Sam Goudsmit was already a famous theoretical physicist in his thirties, mainly because of his co-discovery of electron spin with George Uhlenbeck while both were students of Paul Ehrenfest in Holland in 1925. He and Uhlenbeck continued their thriving careers at the University of Michigan. Goudsmit's style as a physicist was always to make as close a connection between theory and experiment as possible. Thus, for example, his development with his student Robert Bacher of the technique called ``fractional parentage'' used fruitfully in both atomic and nuclear physics to compute energy levels of unknown states in terms of know ones. He also delved deeply into problems related to determinations of nuclear spins and moments. Partly because of his service as scientific leader of the Alsos project at the end of WWII he became a leading statesman of science. I will describe some of his achievements both as a physicist and as a statesman, prior to his becoming Editor in Chief of the American Physical Society.

  2. Sam Shepard and the dysfunctional American family: therapeutic perspectives.

    PubMed

    Sparr, L F; Erstling, S S; Boehnlein, J K

    1990-10-01

    In depicting dysfunctional families Sam Shepard brought a greater intensity level to family portrayals than had previously been seen in modern American theater. In part, his plays appear to reflect the tumultuous tone of the late 1960s and early 1970s when American society was in flux and when the national uncertainty reached down to the basic unit of society, the family. Yet, despite addressing recently emerging social issues, Shepard's plays also depict universal family conflicts. There have been and always will be compelling forces that threaten domestic cohesiveness. While Shepard's families reflect extremely high levels of disorganization, they also demonstrate scenarios recognizable to all family therapists. They reassert the family's power and its influence on individual development. They also indirectly ask us to reflect on our current clinical practice and research. Family therapists need to continue to pay attention the content issues of family organization as well as therapeutic techniques. Shepard's plays remind therapists to look beyond internal dynamics in order to consider connections and affiliations that integrate families with outside communities. He underscores the importance of knowing the meaning and context of traditional rites of passage within families. Family therapists or other care providers may unwittingly undermine the significance of these family rituals by prescription of "expert" advice.

  3. CD and NMR conformational studies of a peptide encompassing the Mid Loop interface of Ship2-Sam.

    PubMed

    Mercurio, Flavia A; Scognamiglio, Pasqualina L; Di Natale, Concetta; Marasco, Daniela; Pellecchia, Maurizio; Leone, Marilisa

    2014-11-01

    The lipid phosphatase Ship2 is a protein that intervenes in several diseases such as diabetes, cancer, neurodegeneration, and atherosclerosis. It is made up of a catalytic domain and several protein docking modules such as a C-terminal Sam (Sterile alpha motif) domain. The Sam domain of Ship2 (Ship2-Sam) binds to the Sam domains of the EphA2 receptor (EphA2-Sam) and the PI3K effector protein Arap3 (Arap3-Sam). These heterotypic Sam-Sam interactions occur through formation of dimers presenting the canonical "Mid Loop/End Helix" binding mode. The central region of Ship2-Sam, spanning the C-terminal end of α2, the α3 and α4 helices together with the α2α3 and α3α4 interhelical loops, forms the Mid Loop surface that is needed to bind partners Sam domains. A peptide encompassing most of the Ship2-Sam Mid Loop interface (Shiptide) capable of binding to both EphA2-Sam and Arap3-Sam, was previously identified. Here we investigated the conformational features of this peptide, through solution CD and NMR studies in different conditions. These studies reveal that the peptide is highly flexible in aqueous buffer, while it adopts a helical conformation in presence of 2,2,2-trifluoroethanol. The discovered structural insights and in particular the identification of a helical motif, may lead to the design of more constrained and possibly cell permeable Shiptide analogs that could work as efficient antagonists of Ship2-Sam heterotypic interactions and embrace therapeutic applications.

  4. Development of and flight results from the Space Acceleration Measurement System (SAMS)

    NASA Technical Reports Server (NTRS)

    Delombard, Richard; Finley, Brian D.; Baugher, Charles R.

    1992-01-01

    Described here is the development of and the flight results from the Space Acceleration Measurement System (SAMS) flight units used in the Orbiter middeck, Spacelab module, and the Orbitercargo bay. The SAMS units are general purpose microgravity accelerometers designed to support a variety of science experiments with microgravity acceleration measurements. A total of six flight units have been fabricated; four for use in the Orbiter middeck and Spacelab module, and two for use in the Orbiter cargo bay. The design of the units is briefly described. The initial two flights of SAMS units on STS-40 (June 1991) and STS-43 (August 1991) resulted in 371 megabytes and 2.6 gigabytes of data respectively. Analytical techniques developed to examine this quantity of acceleration data are described and sample plots of analyzed data are illustrated. Future missions for the SAMS units are listed.

  5. Introduction to the thematic minireview series on radical S-adenosylmethionine (SAM) enzymes.

    PubMed

    Banerjee, Ruma

    2015-02-13

    In the early days, radical enzyme reactions that use S-adenosylmethionine (SAM) coordinated to an Fe-S cluster, which Perry Frey described as a "poor man's coenzyme B12," were believed to be relatively rare chemical curiosities. Today, bioinformatics analyses have revealed the wide prevalence and sheer numbers of radical SAM enzymes, conferring superfamily status. In this thematic minireview series, the JBC presents six articles on radical SAM enzymes that accomplish wide-ranging chemical transformations. We learn that despite the diversity of the reactions catalyzed, family members share some common structural and mechanistic themes. Still in its infancy, continued explorations promise to be fertile grounds for discoveries that will undoubtedly further broaden our understanding of the catalytic repertoire and deepen our understanding of the chemical strategies used by radical SAM enzymes. PMID:25477525

  6. Radical SAM enzymes in the biosynthesis of sugar-containing natural products.

    PubMed

    Ruszczycky, Mark W; Ogasawara, Yasushi; Liu, Hung-Wen

    2012-11-01

    Carbohydrates play a key role in the biological activity of numerous natural products. In many instances their biosynthesis requires radical mediated rearrangements, some of which are catalyzed by radical SAM enzymes. BtrN is one such enzyme responsible for the dehydrogenation of a secondary alcohol in the biosynthesis of 2-deoxystreptamine. DesII is another example that catalyzes a deamination reaction necessary for the net C4 deoxygenation of a glucose derivative en route to desosamine formation. BtrN and DesII represent the two most extensively characterized radical SAM enzymes involved in carbohydrate biosynthesis. In this review, we summarize the biosynthetic roles of these two enzymes, their mechanisms of catalysis, the questions that have arisen during these investigations and the insight they can offer for furthering our understanding of radical SAM enzymology. This article is part of a Special Issue entitled: Radical SAM enzymes and Radical Enzymology. PMID:22172915

  7. Radical SAM enzymes in the biosynthesis of sugar-containing natural products☆

    PubMed Central

    Ruszczycky, Mark W.; Ogasawara, Yasushi; Liu, Hung-wen

    2012-01-01

    Carbohydrates play a key role in the biological activity of numerous natural products. In many instances their biosynthesis requires radical mediated rearrangements, some of which are catalyzed by radical SAM enzymes. BtrN is one such enzyme responsible for the dehydrogenation of a secondary alcohol in the biosynthesis of 2-deoxystreptamine. DesII is another example that catalyzes a deamination reaction necessary for the net C4 deoxygenation of a glucose derivative en route to desosamine formation. BtrN and DesII represent the two most extensively characterized radical SAM enzymes involved in carbohydrate biosynthesis. In this review, we summarize the biosynthetic roles of these two enzymes, their mechanisms of catalysis, the questions that have arisen during these investigations and the insight they can offer for furthering our understanding of radical SAM enzymology. This article is part of a Special Issue entitled: Radical SAM enzymes and Radical Enzymology. PMID:22172915

  8. SAmBA: an interactive software for optimizing the design of biological macromolecules crystallization experiments.

    PubMed

    Audic, S; Lopez, F; Claverie, J M; Poirot, O; Abergel, C

    1997-10-01

    SAmBA is a new software for the design of minimal experimental protocols using the notion of orthogonal arrays of strength 2. The main application of SAmBA is the search of protein crystallization conditions. Given a user input defining the relevant effectors/variables (e.g., pH, temperature, salts) and states (e.g., pH: 5, 6, 7 and 8), this software proposes an optimal set of experiments in which all tested variables and the pairwise interactions between them are symmetrically sampled. No a priori restrictions on the number and range of experimental variables is imposed. SAmBA consists of two complementary programs, SAm and BA, using a simulated annealing approach and a backtracking algorithm, respectively. The software is freely available as C code or as an interactive JAVA applet at http:/(/)igs-server.cnrs-mrs.fr. PMID:9329089

  9. Radical SAM enzymes involved in the biosynthesis of purine-based natural products

    PubMed Central

    Bandarian, Vahe

    2013-01-01

    The radical S-adenosyl-L-methionine (SAM) superfamily is a widely distributed group of iron-sulfur containing proteins that exploit the reactivity of the high energy intermediate, 5’-deoxyadenosyl radical, which is produced by reductive cleavage of SAM, to carry-out complex radical-mediated transformations. The reactions catalyzed by radical SAM enzymes range from simple group migrations to complex reactions in protein and RNA modification. This review will highlight three radical SAM enzymes that catalyze reactions involving modified guanosines in the biosynthesis pathways of the hypermodified tRNA base wybutosine; secondary metabolites of 7-deazapurine structure, including the hypermodified tRNA base queuosine; and the redox cofactor F420. PMID:22902275

  10. Tandem SAM Domain Structure of Human Caskin1: A Presynaptic, Self-Assembling Scaffold for CASK

    SciTech Connect

    Stafford, Ryan L.; Hinde, Elizabeth; Knight, Mary Jane; Pennella, Mario A.; Ear, Jason; Digman, Michelle A.; Gratton, Enrico; Bowie, James U.

    2012-02-07

    The synaptic scaffolding proteins CASK and Caskin1 are part of the fibrous mesh of proteins that organize the active zones of neural synapses. CASK binds to a region of Caskin1 called the CASK interaction domain (CID). Adjacent to the CID, Caskin1 contains two tandem sterile a motif (SAM) domains. Many SAM domains form polymers so they are good candidates for forming the fibrous structures seen in the active zone. We show here that the SAM domains of Caskin1 form a new type of SAM helical polymer. The Caskin1 polymer interface exhibits a remarkable segregation of charged residues, resulting in a high sensitivity to ionic strength in vitro. The Caskin1 polymers can be decorated with CASK proteins, illustrating how these proteins may work together to organize the cytomatrix in active zones.

  11. Bringing a Chemical Laboratory Named Sam to Mars on the 2011 Curiosity Rover

    NASA Astrophysics Data System (ADS)

    Mahaffy, P. R.; Bleacher, L.; Jones, A.; Atreya, S. K.; Manning, H. L.; Cabane, M.; Webster, C. R.; Sam Team

    2010-12-01

    Introduction: An important goal of upcoming missions to Mars is to understand if life could have developed there. The task of the Sample Analysis at Mars (SAM) suite of instruments [1] and the other Curiosity investigations [2] is to move us steadily toward that goal with an assessment of the habitability of our neighboring planet through a series of chemical and geological measurements. SAM is designed to search for organic compounds and inorganic volatiles and measure isotope ratios. Other instruments on Curiosity will provide elemental analysis and identify minerals. SAM will analyze both atmospheric samples and gases evolved from powdered rocks that may have formed billions of years ago with Curiosity providing access to interesting sites scouted by orbiting cameras and spectrometers. SAM Instrument Suite: SAM’s instruments are a Quadrupole Mass Spectrometer (QMS), a 6-column Gas Chromatograph (GC), and a 2-channel Tunable Laser Spectrometer (TLS). SAM can identify organic compounds in Mars rocks to sub-ppb sensitivity and secure precise isotope ratios for C, H, and O in carbon dioxide and water and measure trace levels of methane and its carbon 13 isotope. The SAM gas processing system consists of valves, heaters, pressure sensors, gas scrubbers and getters, traps, and gas tanks used for calibration or combustion experiments [2]. A variety of calibrant compounds interior and exterior to SAM will allow the science and engineering teams to assess SAM’s performance. SAM has been calibrated and tested in a Mars-like environment. Keeping Educators and the Public Informed: The Education and Public Outreach (EPO) goals of the SAM team are to make this complex chemical laboratory and its data widely available to educators, students, and the public. Formal education activities include developing templates for professional development workshops for educators to teach them about SAM and Curiosity, incorporating data into Mars Student Data Teams, and writing articles

  12. Inkless microcontact printing on SAMs of Boc- and TBS-protected thiols.

    PubMed

    Shestopalov, Alexander A; Clark, Robert L; Toone, Eric J

    2010-01-01

    We report a new inkless catalytic muCP technique that achieves accurate, fast, and complete pattern reproduction on SAMs of Boc- and TBS-protected thiols immobilized on gold using a polyurethane-acrylate stamp functionalized with covalently bound sulfonic acids. Pattern transfer is complete at room temperature just after one minute of contact and renders sub-200 nm size structures of chemically differentiated SAMs.

  13. The Combustion Experiment on the Sample Analysis at Mars (SAM) Instrument Suite on the Curiosity Rover

    NASA Technical Reports Server (NTRS)

    Stern, J. C.; Malespin, C. A.; Eigenbrode, J. L.; Graham, H. V.; Archer, P. D., Jr.; Brunner, A. E.; Freissinet, C.; Franz, H. B.; Fuentes, J.; Glavin, D. P.; Leshin, L. A.; Mahaffy, P. R.; McAdam, A. C.; Ming, D. W.; Navvaro-Gonzales, R.; Niles, P. B.; Steele, A.

    2014-01-01

    The combustion experiment on the Sample Analysis at Mars (SAM) suite on Curiosity will heat a sample of Mars regolith in the presence of oxygen and measure composition of the evolved gases using quadrupole mass spectrometry (QMS) and tunable laser spectrometry (TLS). QMS will enable detection of combustion products such as CO, CO2, NO, and other oxidized species, while TLS will enable precise measurements of the abundance and carbon isotopic composition (delta(sup 13)C) of the evolved CO2 and hydrogen isotopic composition (deltaD) of H2O. SAM will perform a two-step combustion to isolate combustible materials below approx.550 C and above approx.550 C. The combustion experiment on SAM, if properly designed and executed, has the potential to answer multiple questions regarding the origins of volatiles seen thus far in SAM evolved gas analysis (EGA) on Mars. Constraints imposed by SAM and MSL time and power resources, as well as SAM consumables (oxygen gas), will limit the number of SAM combustion experiments, so it is imperative to design an experiment targeting the most pressing science questions. Low temperature combustion experiments will primarily target the quantification of carbon (and nitrogen) contributed by SAM wet chemistry reagants MTBSTFA (N-Methyl-N-tert-butyldimethylsilyltrifluoroacetamide) and DMF (Dimethylformamide), which have been identified in the background of blank and sample runs and may adsorb to the sample while the cup is in the Sample Manipulation System (SMS). In addition, differences between the sample and "blank" may yield information regarding abundance and delta(sup 13)C of bulk (both organic and inorganic) martian carbon. High temperature combustion experiments primarily aim to detect refractory organic matter, if present in Cumberland fines, as well as address the question of quantification and deltaD value of water evolution associated with hydroxyl hydrogen in clay minerals.

  14. Identification of trans-acting factors regulating SamDC expression in Oryza sativa

    SciTech Connect

    Basu, Supratim; Roychoudhury, Aryadeep; Sengupta, Dibyendu N.

    2014-03-07

    Highlights: • Identification of cis elements responsible for SamDC expression by in silico analysis. • qPCR analysis of SamDC expression to abiotic and biotic stress treatments. • Detection of SamDC regulators using identified cis-elements as probe by EMSA. • Southwestern Blot analysis to predict the size of the trans-acting factors. - Abstract: Abiotic stress affects the growth and productivity of crop plants; to cope with the adverse environmental conditions, plants have developed efficient defense machinery comprising of antioxidants like phenolics and flavonoids, and osmolytes like polyamines. SamDC is a key enzyme in the polyamine biosynthesis pathway in plants. In our present communication we have done in silico analysis of the promoter region of SamDC to look for the presence of different cis-regulatory elements contributing to its expression. Based on the presence of different cis-regulatory elements we completed comparative analysis of SamDC gene expression in rice lamina of IR-29 and Nonabokra by qPCR in response to the abiotic stress treatments of salinity, drought, cold and the biotic stress treatments of ABA and light. Additionally, to explore the role of the cis-regulatory elements in regulating the expression of SamDC gene in plants we comparatively analyzed the binding of rice nuclear proteins prepared from IR-29 and Nonabokra undergoing various stress treatments. The intensity of the complex formed was low and inducible in IR-29 in contrast to Nonabokra. Southwestern blot analysis helped in predicting the size of the trans-acting factors binding to these cis-elements. To our knowledge this is the first report on the comprehensive analysis of SamDC gene expression in rice and identification of the trans-acting factors regulating its expression.

  15. Contribution of sams-1 and pmt-1 to lipid homoeostasis in adult Caenorhabditis elegans.

    PubMed

    Li, Yingxiu; Na, Keun; Lee, Hyoung-Joo; Lee, Eun-Young; Paik, Young-Ki

    2011-05-01

    Accumulation of lipids inside the cell is primarily caused by disorders of lipid metabolism. S-adenosylmethionine synthetase (SAMS) produces SAM, an important methyl donor in various phospholipid methyltransferase reactions catalysed by phosphoethanolamine N-methyltransferase (PMT-1). A gel-based, quantitative proteomic analysis of the RNA interference (RNAi)-mediated inactivation of the pod-2 gene, which encodes acetyl-CoA carboxylase, showed a substantial down-regulation of SAMS-1. Consequently, RNAi of either sams-1 or pmt-1 caused a significant increase in lipid droplet size in the intestine of Caenorhabditis elegans. Lipid droplets exhibited increased triacylglycerol (TG) and decreased phosphatidylcholine (PC) levels, suggesting a reciprocal relationship between TG and PC regulation. These lipid-associated phenotypes were rescued by choline feeding. Among the five fat metabolism-related genes examined, two genes were highly induced by inactivation of sams-1 or pmt-1: pod-2 and stearoyl-CoA desaturase (fat-7). Thus, both SAMS-1 and PMT-1 were shown to contribute to the homoeostasis of TG and PC levels in C. elegans, which would provide an important survival strategy under harsh environmental conditions.

  16. Non-canonical active site architecture of the radical SAM thiamin pyrimidine synthase

    DOE PAGES

    Fenwick, Michael K.; Mehta, Angad P.; Zhang, Yang; Abdelwahed, Sameh H.; Begley, Tadhg P.; Ealick, Steven E.

    2015-03-27

    Radical S-adenosylmethionine (SAM) enzymes use a [4Fe-4S] cluster to generate a 5'-deoxyadenosyl radical. Canonical radical SAM enzymes are characterized by a β-barrel-like fold and SAM anchors to the differentiated iron of the cluster, which is located near the amino terminus and within the β-barrel, through its amino and carboxylate groups. Here we show that ThiC, the thiamin pyrimidine synthase in plants and bacteria, contains a tethered cluster-binding domain at its carboxy terminus that moves in and out of the active site during catalysis. In contrast to canonical radical SAM enzymes, we predict that SAM anchors to an additional active sitemore » metal through its amino and carboxylate groups. Superimposition of the catalytic domains of ThiC and glutamate mutase shows that these two enzymes share similar active site architectures, thus providing strong evidence for an evolutionary link between the radical SAM and adenosylcobalamin-dependent enzyme superfamilies.« less

  17. Non-canonical active site architecture of the radical SAM thiamin pyrimidine synthase

    NASA Astrophysics Data System (ADS)

    Fenwick, Michael K.; Mehta, Angad P.; Zhang, Yang; Abdelwahed, Sameh H.; Begley, Tadhg P.; Ealick, Steven E.

    2015-03-01

    Radical S-adenosylmethionine (SAM) enzymes use a [4Fe-4S] cluster to generate a 5‧-deoxyadenosyl radical. Canonical radical SAM enzymes are characterized by a β-barrel-like fold and SAM anchors to the differentiated iron of the cluster, which is located near the amino terminus and within the β-barrel, through its amino and carboxylate groups. Here we show that ThiC, the thiamin pyrimidine synthase in plants and bacteria, contains a tethered cluster-binding domain at its carboxy terminus that moves in and out of the active site during catalysis. In contrast to canonical radical SAM enzymes, we predict that SAM anchors to an additional active site metal through its amino and carboxylate groups. Superimposition of the catalytic domains of ThiC and glutamate mutase shows that these two enzymes share similar active site architectures, thus providing strong evidence for an evolutionary link between the radical SAM and adenosylcobalamin-dependent enzyme superfamilies.

  18. Sam68 Is Required for DNA Damage Responses via Regulating Poly(ADP-ribosyl)ation

    PubMed Central

    Hodgson, Andrea; Wier, Eric M.; Wen, Matthew G.; Kamenyeva, Olena; Xia, Xue; Koo, Lily Y.

    2016-01-01

    The rapid and robust synthesis of polymers of adenosine diphosphate (ADP)-ribose (PAR) chains, primarily catalyzed by poly(ADP-ribose) polymerase 1 (PARP1), is crucial for cellular responses to DNA damage. However, the precise mechanisms through which PARP1 is activated and PAR is robustly synthesized are not fully understood. Here, we identified Src-associated substrate during mitosis of 68 kDa (Sam68) as a novel signaling molecule in DNA damage responses (DDRs). In the absence of Sam68, DNA damage-triggered PAR production and PAR-dependent DNA repair signaling were dramatically diminished. With serial cellular and biochemical assays, we demonstrated that Sam68 is recruited to and significantly overlaps with PARP1 at DNA lesions and that the interaction between Sam68 and PARP1 is crucial for DNA damage-initiated and PARP1-conferred PAR production. Utilizing cell lines and knockout mice, we illustrated that Sam68-deleted cells and animals are hypersensitive to genotoxicity caused by DNA-damaging agents. Together, our findings suggest that Sam68 plays a crucial role in DDR via regulating DNA damage-initiated PAR production. PMID:27635653

  19. Sam68 Is Required for DNA Damage Responses via Regulating Poly(ADP-ribosyl)ation.

    PubMed

    Sun, Xin; Fu, Kai; Hodgson, Andrea; Wier, Eric M; Wen, Matthew G; Kamenyeva, Olena; Xia, Xue; Koo, Lily Y; Wan, Fengyi

    2016-09-01

    The rapid and robust synthesis of polymers of adenosine diphosphate (ADP)-ribose (PAR) chains, primarily catalyzed by poly(ADP-ribose) polymerase 1 (PARP1), is crucial for cellular responses to DNA damage. However, the precise mechanisms through which PARP1 is activated and PAR is robustly synthesized are not fully understood. Here, we identified Src-associated substrate during mitosis of 68 kDa (Sam68) as a novel signaling molecule in DNA damage responses (DDRs). In the absence of Sam68, DNA damage-triggered PAR production and PAR-dependent DNA repair signaling were dramatically diminished. With serial cellular and biochemical assays, we demonstrated that Sam68 is recruited to and significantly overlaps with PARP1 at DNA lesions and that the interaction between Sam68 and PARP1 is crucial for DNA damage-initiated and PARP1-conferred PAR production. Utilizing cell lines and knockout mice, we illustrated that Sam68-deleted cells and animals are hypersensitive to genotoxicity caused by DNA-damaging agents. Together, our findings suggest that Sam68 plays a crucial role in DDR via regulating DNA damage-initiated PAR production. PMID:27635653

  20. 75 FR 56055 - Advisory Committee and Species Working Group Technical Advisor Appointments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-15

    ... Atlantic Tunas (ICCAT) as established by the Atlantic Tunas Convention Act of 1975 (ATCA). NMFS is also... Bluefin Tuna Working Group, a Swordfish and Sharks Working Group, a Billfish Working Group, and a BAYS (Bigeye, Albacore, Yellowfin, and Skipjack) Tunas Working Group. Technical Advisors to the species...

  1. The Advisor as Servant: The Theoretical and Philosophical Relevance of Servant Leadership to Academic Advising

    ERIC Educational Resources Information Center

    McClellan, Jeffrey L.

    2007-01-01

    Servant. This novel actively portrays Greenleaf's concept of servant leadership by describing the extracurricular work of a university professor. Consequently, some scholars have demonstrated the relevance of servant leadership to classroom instruction (Powers & Moore, 2005). However, it was not as an instructor, but as an advisor that the…

  2. Evaluation of a College Curriculum Advisory Program Utilizing Student Advisors. Final Report.

    ERIC Educational Resources Information Center

    Brown, Coke R.

    This study was designed to evaluate the viability of using students as academic advisors for freshmen students and to document the process of the advising program at Idaho State University. Results show that students advised by students did as well academically and had a lower dropout rate than students advised by faculty. Descriptions of advisor…

  3. Reframing Doctoral Programs: A Program of Human Inquiry for Doctoral Students and Faculty Advisors.

    ERIC Educational Resources Information Center

    Shambaugh, R. Neal

    2000-01-01

    Proposes the Program of Human Inquiry as a framework for joint student-faculty portfolios by graduate students and faculty advisors. The program consists of four components: (1) acknowledgment of what one brings to graduate studies; (2) a plan of study, (3) a record of rigorous negotiated "avenues of inquiry," and (4) ongoing discussion of values…

  4. 76 FR 14379 - Subcommittee Meeting of the Board of Advisors to the President, Naval Postgraduate School

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-16

    ... the following meeting of the Subcommittee Meeting of the Board of Advisors (BOA) to The President... deliberations leading to provisional findings for referral to the BOA to the Presidents of the NPS and the Naval... the NPS BOA contact Ms. Jaye Panza, Designated Federal Officer, Naval Postgraduate School,...

  5. Succeeding in Undergraduate Student Research: A Few Helpful Hints for Advisors

    ERIC Educational Resources Information Center

    Billings, Lora

    2013-01-01

    This article offers some insights into successfully engaging students in research. While most schools encourage undergraduate research, there is little guidance specific to mathematics on how to make it a rewarding experience for both the student and the advisor. With a small support group and a goal-oriented time line, students will be able to…

  6. Graduate Students' Perceptions of Their Advisors: Is There Systematic Disadvantage in Mentorship?

    ERIC Educational Resources Information Center

    Noy, Shiri; Ray, Rashawn

    2012-01-01

    We explore how race and gender shape graduate students' perceptions of their advisors. We find evidence that women of color and students in the biological/physical sciences report significantly less support than other groups. Our findings speak to the utility of the intersectionality framework for examining interpersonal relations in higher…

  7. Accommodations in Teacher Education: Perspectives of Teacher Candidates with Learning Disabilities and Their Faculty Advisors

    ERIC Educational Resources Information Center

    Csoli, Karen; Gallagher, Tiffany L.

    2012-01-01

    This study of teacher candidates with learning disabilities profiles their experience in a teacher education program. Two teacher candidates and their faculty advisors offer perspectives at various points during the teacher education program. Findings indicate that the teacher candidates were able to complete their required courses when their…

  8. 77 FR 5065 - Preservation Trust Advisors, LLC and Northern Lights Fund Trust; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-01

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION Preservation Trust Advisors, LLC and Northern Lights Fund Trust; Notice of Application January 26...'') and Northern Lights Fund Trust (the ``Trust''). FILING DATES: The application was filed on...

  9. 77 FR 5585 - Northwestern Mutual Series Fund, Inc. and Mason Street Advisors, LLC; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-03

    ... Advisors, LLC (``MSA''). DATES: Filing Dates: The application was filed on November 30, 2011. Applicants... Act as an open-end management investment company. MSA, a Delaware limited liability company, is an...) is advised by MSA or any person now or in the future controlling, controlled by or under...

  10. Influence of an Identified Advisor/Mentor on Urban Latino Students' College Experience

    ERIC Educational Resources Information Center

    Torres, Vasti; Hernandez, Ebelia

    2010-01-01

    This study considers the scales previously used by Nora, Kraemer, and Itzen (1997) and Torres (2006) as contributing to the retention of Latino/a college students. T-tests were used to compare the scale means between students with an identified advisor or mentor and those that have not identified one. The data is from Latino college students at…

  11. Architects, Captains, and Dreamers: Creating Advisor Roles that Foster Youth-Adult Partnerships

    ERIC Educational Resources Information Center

    Mitra, Dana; Lewis, Tiffanie; Sanders, Felicia

    2013-01-01

    While research has documented the many ways in which student voice can enable educational change, the process of how adults can help to enable student voice is less clear. This article examines how adults new to working as advisors of student voice initiatives begin to develop partnerships with young people. Using a Youth-Adult Partnership…

  12. 76 FR 60564 - President's Council of Advisors on Science and Technology; Notice of Meeting: Open Regional...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-29

    ... government create a fertile environment for innovation and make investments to ensure that new technologies... issues where understandings from the domains of science, technology, and innovation may bear on the... TECHNOLOGY POLICY President's Council of Advisors on Science and Technology; Notice of Meeting: Open...

  13. 20 CFR 404.942 - Prehearing proceedings and decisions by attorney advisors.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Prehearing proceedings and decisions by attorney advisors. 404.942 Section 404.942 Employees' Benefits SOCIAL SECURITY ADMINISTRATION FEDERAL OLD-AGE, SURVIVORS AND DISABILITY INSURANCE (1950- ) Determinations, Administrative Review Process,...

  14. The Moderating Effect of Personality Traits on Advisor Relationships in Predicting Doctoral Student Burnout

    ERIC Educational Resources Information Center

    Kosh, Emily P.

    2014-01-01

    Personality affects relationships. During the doctoral education, the second most important factor in degree completion, after financial support, is the student-advisor relationship. Approximately half of doctoral students do not finish their degrees. While it is known mentors have a profound impact on the success of doctoral students, the effect…

  15. Teachers Providing Social and Emotional Support: A Study of Advisor Role Enactment in Small High Schools

    ERIC Educational Resources Information Center

    Phillippo, Kate L.

    2010-01-01

    Background/Context: This study investigates the teacher's role in the student advisory process, which to date has generated limited research literature. Teachers who serve as student advisors assume a role that extends beyond the more traditional instructional role, and includes implied or explicit expectations to provide student advisees with…

  16. A Case Study on Two Advisor-Advisee Relationships in a Master's TESOL Program in Taiwan

    ERIC Educational Resources Information Center

    Chen, Cheryl Wei-yu

    2010-01-01

    Situated in an MA TESOL program in a large Taiwanese university, this study is a qualitative case study on two advising relationships. Three central issues were examined closely: (a) the nature of the advising relationships; (b) the challenges students encountered in the process of completing their theses; and (c) the advisors and advisee's…

  17. A National Study Examining the Impact of SEVIS on International Student and Scholar Advisors

    ERIC Educational Resources Information Center

    Rosser, Vicki J.; Hermsen, Jill M.; Mamiseishvili, Ketevan; Wood, Melinda S.

    2007-01-01

    The purpose of this research is to examine the impact that Student and Exchange Visitor Information System (SEVIS) is having on the professional worklives of international student and scholar advisors (ISSAs) on U.S. college and university campuses. ISSAs are clearly satisfied with their work and hold their institutions in high regard. However,…

  18. 75 FR 17912 - Sunshine Act; Notice of Virtual Public Forum for EAC Board of Advisors

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-08

    ... From the Federal Register Online via the Government Publishing Office ELECTION ASSISTANCE COMMISSION Sunshine Act; Notice of Virtual Public Forum for EAC Board of Advisors Date & Time: Monday, April... Information: Bryan Whitener, Telephone: (202) 566-3100. ] Signed: Gracia Hillman, Commissioner, U.S....

  19. Installation and evaluation of a nuclear power plant operator advisor based on artificial intelligence technology

    SciTech Connect

    Hajek, B.K.; Miller, D.W.

    1989-06-20

    This report discusses the following topics on a Nuclear Power Plant operator advisor based on artificial Intelligence Technology; Workstation conversion; Software Conversion; V V Program Development Development; Simulator Interface Development; Knowledge Base Expansion; Dynamic Testing; Database Conversion; Installation at the Perry Simulator; Evaluation of Operator Interaction; Design of Man-Machine Interface; and Design of Maintenance Facility.

  20. WASTE ANALYSIS PLAN REVIEW ADVISOR - AN INTELLIGENT DATABASE TO ASSIST RCRA PERMIT REVIEWERS

    EPA Science Inventory

    The Waste Analysis Plan Review Advisor (WAPRA) system assists in the review of the Waste Analysis Plan Section of RCRA Part B facility permit applications. Specifically, this program automates two functions of the waste analysis plan review. First, the system checks all wastes wh...

  1. Use of Appreciative Inquiry to Explore the Experiences of Faculty Advisors

    ERIC Educational Resources Information Center

    Myers, Valerie Ann-Marie

    2013-01-01

    Academic advising significantly impacts student achievement, persistence, and retention, yet it continues to be an area of weakness for many institutions. The purpose of this sequential mixed-methods case study was to explore the experiences of faculty advisors to identify strengths and weaknesses in their knowledge and skills. Appreciative…

  2. A Survey of Attitude and Perception of the Graduate Student of his Advisor or Committee Chairman.

    ERIC Educational Resources Information Center

    Stewart, James W.

    Current graduate education, with its focus on tailoring programs for individual students, has made the relationship between the student and his advisor or committee chairman an essential ingredient of graduate programs. It is this relationship which the survey examines. Specifically, its purpose is to identify and examine graduate students'…

  3. 75 FR 35851 - Partially Closed Meeting of the President's Council of Advisors on Science and Technology

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-23

    ... scheduled to hear presentations on space policy and science, technology, ] and diplomacy. PCAST members will... From the Federal Register Online via the Government Publishing Office OFFICE OF SCIENCE AND TECHNOLOGY POLICY Partially Closed Meeting of the President's Council of Advisors on Science and...

  4. 78 FR 68040 - President's Council of Advisors on Science and Technology Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-13

    ... Science and Technology, and Director, Office of Science and Technology Policy, Executive Office of the... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY President's Council of Advisors on Science and Technology Meeting AGENCY: Department of Energy....

  5. Bridging Horizons. An Advisor's Guide to FFA Involvement for Members with Disabilities.

    ERIC Educational Resources Information Center

    Ploss, Adrienne J.; Field, William E.; Frick, Martin J.

    This guide is designed to provide Future Farmers of America (FFA) advisors with information to assist them in their efforts to include all youth in FFA, including those with disabilities. It addresses benefits of involving youth with disabilities and federal, state, and local legislation and regulations concerning people with disabilities.…

  6. 76 FR 62871 - President's Council of Advisors on Science and Technology Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-11

    ... TECHNOLOGY POLICY President's Council of Advisors on Science and Technology Meeting AGENCY: Office of Science... Science and Technology (PCAST) is an advisory group of the nation's leading scientists and engineers, appointed by the President to augment the science and technology advice available to him from inside...

  7. A Community Health Advisor Program to Reduce Cardiovascular Risk among Rural African-American Women

    ERIC Educational Resources Information Center

    Cornell, C. E.; Littleton, M. A.; Greene, P. G.; Pulley, L.; Brownstein, J. N.; Sanderson, B. K.; Stalker, V. G.; Matson-Koffman, D.; Struempler, B.; Raczynski, J. M.

    2009-01-01

    The Uniontown, Alabama Community Health Project trained and facilitated Community Health Advisors (CHAs) in conducting a theory-based intervention designed to reduce the risk for cardiovascular disease (CVD) among rural African-American women. The multiphased project included formative evaluation and community organization, CHA recruitment and…

  8. Ties That Bind: Academic Advisors as Agents of Student Relationship Management

    ERIC Educational Resources Information Center

    Vianden, Jörg

    2016-01-01

    To affect college retention, academic advisors should act as agents of student relationship management by strengthening the connection between students and their institutions. Satisfaction and dissatisfaction with academic advising as perceived by 29 college students at 3 midwestern comprehensive institutions are described. Discussion is framed in…

  9. MySci Advisors: Establishing a Peer-Mentoring Program for First Year Science Student Support

    ERIC Educational Resources Information Center

    Poling, Kirsten

    2015-01-01

    Would you like to help your students adjust to university life? Perhaps you are simply interested in allowing them to feel more integrated into a department right from the start of their first year? These were the types of issues that we were hoping to address when we founded the MySci Advisors Program, a peer-mentoring group for first year…

  10. 78 FR 23242 - President's Council of Advisors on Science and Technology (PCAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-18

    ... President's Council of Advisors on Science and Technology (PCAST) AGENCY: Office of Science, Department of... President to augment the science and technology advice available to him from inside the White House and from... issues where understandings from the domains of science, technology, and innovation may bear on...

  11. Characteristics of and Challenges for Advisors within a Privatized Extension System

    ERIC Educational Resources Information Center

    Knuth, Ulrike; Knierim, Andrea

    2013-01-01

    Purpose: The aim of this article is to provide evidence on the consequences of the privatization process in Brandenburg with a focus on the agricultural advisors' situation. Before the background of future European Union (EU) expectations on Farm Advisory Systems (FAS), their capacities and competences to respond to such challenges are discussed.…

  12. 40 CFR 35.4190 - How does my group identify a qualified technical advisor?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... health, environmental sciences, engineering, environmental law and planning); and (3) Ability to... 40 Protection of Environment 1 2010-07-01 2010-07-01 false How does my group identify a qualified technical advisor? 35.4190 Section 35.4190 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY...

  13. 40 CFR 35.4190 - How does my group identify a qualified technical advisor?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... health, environmental sciences, engineering, environmental law and planning); and (3) Ability to... 40 Protection of Environment 1 2013-07-01 2013-07-01 false How does my group identify a qualified technical advisor? 35.4190 Section 35.4190 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY...

  14. 40 CFR 35.4190 - How does my group identify a qualified technical advisor?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... health, environmental sciences, engineering, environmental law and planning); and (3) Ability to... 40 Protection of Environment 1 2011-07-01 2011-07-01 false How does my group identify a qualified technical advisor? 35.4190 Section 35.4190 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY...

  15. 40 CFR 35.4190 - How does my group identify a qualified technical advisor?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... health, environmental sciences, engineering, environmental law and planning); and (3) Ability to... 40 Protection of Environment 1 2014-07-01 2014-07-01 false How does my group identify a qualified technical advisor? 35.4190 Section 35.4190 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY...

  16. 40 CFR 35.4190 - How does my group identify a qualified technical advisor?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... health, environmental sciences, engineering, environmental law and planning); and (3) Ability to... 40 Protection of Environment 1 2012-07-01 2012-07-01 false How does my group identify a qualified technical advisor? 35.4190 Section 35.4190 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY...

  17. Crop advisor perceptions of giant ragweed distribution, herbicide-resistance, and management in the Corn Belt

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Giant ragweed has been increasing as a major weed of row crops in North America. We conducted a web-based survey of Certified Crop Advisors in the Corn Belt to determine the current distribution of giant ragweed, the distribution of herbicide resistant populations, and management and ecological fact...

  18. 75 FR 4054 - Sunshine Act; Notice of Virtual Public Forum for EAC Board of Advisors

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-26

    ... COMMISSION Sunshine Act; Notice of Virtual Public Forum for EAC Board of Advisors DATE & TIME: Monday... efficient manner in a public forum, including being able to review and discuss draft documents, when it is... any resolutions during the 12-day forum of February 8-February 19, 2010. Members will post...

  19. Perceptions and Practices of Technology Student Association Advisors on Implementation Strategies and Teaching Methods

    ERIC Educational Resources Information Center

    Haynie, W. J.; DeLuca, V. W.; Matthews, B.

    2005-01-01

    A study conducted in 1989 surveyed Technology Student Association (TSA) advisors to find their perceptions concerning characteristics of technology education programs with a TSA component and the relationship between participation in co-curricular organizations and the teaching methods used by TSA technology teachers (DeLuca & Haynie, 1991).…

  20. 77 FR 25210 - Advisors Series Trust and Orinda Asset Management, LLC; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-27

    ... approved by the shareholders of the applicable Fund. 5. At all times, at least a majority of the Board will... materially amend subadvisory agreements without shareholder approval and would grant relief from certain... organized under Delaware law, and the four managing partners of the Advisor each have a minority interest...

  1. The Influence of the CAS Standards on Academic Advisors and Advising Programs

    ERIC Educational Resources Information Center

    Keeling, Sarah

    2010-01-01

    Research on the influence of the Council for the Advancement of Standards in Higher Education (CAS) on academic advising is limited. Using a comparative case study method, I respond to this research gap by exploring how the standards influence practices of academic advising programs. Study results indicate that participating advisors knew little…

  2. Culturally Competent Training Program: A Key to Training Lay Health Advisors for Promoting Breast Cancer Screening

    ERIC Educational Resources Information Center

    Yu, Mei-yu; Song, Lixin; Seetoo, Amy; Cai, Cuijuan; Smith, Gary; Oakley, Deborah

    2007-01-01

    The lay health advisor (LHA) training program for breast cancer screening was conducted among Chinese-English bilingual trainees residing in Southeast Michigan. Guided by Bandura's Social Learning Theory, the development of the training curriculum followed the health communication process recommended by the National Cancer Institute. Data analysis…

  3. 76 FR 11701 - Amendments to Commodity Pool Operator and Commodity Trading Advisor Regulations Resulting From...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-03

    ... contracts, commodity options, and off-exchange retail foreign currency transactions, and it does not include... would establish business conduct standards for swap dealers and major swap participants. See 75 FR 80638... and Commodity Trading Advisors: Amendments to Compliance Obligations, 76 FR 7976 (Feb. 11, 2011);...

  4. 78 FR 77175 - Catalyst Capital Advisors LLC and Mutual Fund Series Trust; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-20

    ... agreements without shareholder approval. APPLICANTS: Catalyst Capital Advisors LLC (``CCA'' or the ``Adviser... the Trust or Adviser (the ``Independent Trustees''), and by the shareholders of the respective Fund in... into and materially amend Subadvisory Agreements without obtaining shareholder approval. The terms...

  5. Active Region Soft X-Ray Spectra and Temperature Analyses based on Sounding Rocket Measurements from the Solar Aspect Monitor (SAM), - a Modified SDO/EVE Instrument

    NASA Astrophysics Data System (ADS)

    Didkovsky, Leonid V.; Wieman, Seth; Woods, Thomas N.; Jones, Andrew; Moore, Christopher

    2016-05-01

    Some initial results of soft x-ray spectral (0.5 to 3.0 nm) observations of active regions (AR11877 and AR11875) from a sounding rocket flight NASA 36.290 on 21 October 2013 at about 18:30 UT are reported. These observations were made by a Solar Aspect Monitor (SAM), a rocket version of the EUV Variability Experiment’s (EVE) channel, a pinhole camera modified for EVE rocket suite of instruments to include a free-standing transmission grating (200 nm period), which provided spectrally-resolved images of the solar disk. Intensity ratios for strong emission lines extracted from temporally averaged SAM spectral profiles of the ARs were compared to appropriately convolved modeled CHIANTI spectra. These ratios represent the AR’s temperature structures, which are compared to the structures derived from some other observations and temperature models.

  6. Student goal orientation in learning inquiry skills with modifiable software advisors

    NASA Astrophysics Data System (ADS)

    Shimoda, Todd Adrian

    A computer support environment (SCI-WISE) for learning and doing inquiry was designed. The system incorporates software advisors that give task completion advice (eg., forming hypotheses), general purpose advice (e.g., brainstorming), or system modification advice. Advisors' knowledge includes concepts, strategies, examples, referrals to other advisors, and criteria for evaluating good products and skills. Students using SCI-WISE can select the advisors' advice type (specific, general, or hints), and when advisors give advice (anytime, alert, or ask). Students can also modify the advisors' knowledge. The system is designed partly on a theoretical framework that assumes giving students higher levels of agency will facilitate higher-level goal orientation (such as knowledge-building) and produce higher levels of competence. In two studies of sixth graders, science students took a pretest of an open-ended inquiry question and a questionnaire that measured their goal orientations. The students worked in pairs on an inquiry project about memory, using one of two versions of SCI-WISE, one modifiable and one not modifiable. After finishing the project, the students took a posttest similar to the pretest, and evaluated the system. The main hypotheses predicted that knowledge-oriented students using the modifiable version would rate the system higher, use it more effectively, and do better on the inquiry posttest than task-oriented students. The results supported many of the hypotheses generated from the theoretical framework. Knowledge-oriented students tended to rate SCI-WISE higher, use more general purpose and system development advisors, and select more general advice and hints than task-oriented students. On the posttest inquiry test, students with higher goal orientations scored higher on average, particularly when paired with another knowledge oriented student. The studies also showed that goal orientation was not correlated with grade point average. Finally, the

  7. SAM-Like Evolved Gas Analyses of Phyllosilicate Minerals and Applications to SAM Analyses of the Sheepbed Mudstone, Gale Crater, Mars

    NASA Technical Reports Server (NTRS)

    McAdam, A. C.; Franz, H. B.; Mahaffy, P. R.; Eigenbrode, J. L.; Stern, J. C.; Brunner, B.; Sutter, B.; Archer, P. D.; Ming , D. W.; Morris, R. V.; Bish, D. L.; Atreya, S. K.

    2014-01-01

    While in Yellowknife Bay, the Mars Science Laboratory Curiosity rover collected two drilled samples, John Klein (hereafter "JK") and Cumberland ("CB"), from the Sheepbed mudstone, as well as a scooped sample from the Rocknest aeolian bedform ("RN"). These samples were sieved by Curiosity's sample processing system and then several subsamples of these materials were delivered to the Sample Analysis at Mars (SAM) instrument suite and the CheMin X-ray diffraction/X-ray fluorescence instrument. CheMin provided the first in situ X-ray diffraction-based evidence of clay minerals on Mars, which are likely trioctahedral smectites (e.g., Fe-saponite) and comprise 20 wt% of the mudstone samples [1]. SAM's evolved gas analysis (EGA) mass spectrometry analyses of JK and CB subsamples, as well as RN subsamples, detected H2O, CO2, O2, H2, SO2, H2S, HCl, NO, OCS, CS2 and other trace gases evolved during pyrolysis. The identity of evolved gases and temperature( s) of evolution can augment mineral detection by CheMin and place constraints on trace volatile-bearing phases present below the CheMin detection limit or those phases difficult to characterize with XRD (e.g., X-ray amorphous phases). Here we will focus on the SAM H2O data, in the context of CheMin analyses, and comparisons to laboratory SAM-like analyses of several phyllosilicate minerals including smectites.

  8. Probing the nature and resistance of the molecule-electrode contact in SAM-based junctions

    NASA Astrophysics Data System (ADS)

    Suchand Sangeeth, C. S.; Wan, Albert; Nijhuis, Christian A.

    2015-07-01

    It is challenging to quantify the contact resistance and to determine the nature of the molecule-electrode contacts in molecular two-terminal junctions. Here we show that potentiodynamic and temperature dependent impedance measurements give insights into the nature of the SAM-electrode interface and other bottlenecks of charge transport (the capacitance of the SAM (CSAM) and the resistance of the SAM (RSAM)), unlike DC methods, independently of each other. We found that the resistance of the top-electrode-SAM contact for junctions with the form of AgTS-SCn//GaOx/EGaIn with n = 10, 12, 14, 16 or 18 is bias and temperature independent and hence Ohmic (non-rectifying) in nature, and is orders of magnitude smaller than RSAM. The CSAM and RSAM are independent of the temperature, indicating that the mechanism of charge transport in these SAM-based junctions is coherent tunneling and the charge carrier trapping at the interfaces is negligible.It is challenging to quantify the contact resistance and to determine the nature of the molecule-electrode contacts in molecular two-terminal junctions. Here we show that potentiodynamic and temperature dependent impedance measurements give insights into the nature of the SAM-electrode interface and other bottlenecks of charge transport (the capacitance of the SAM (CSAM) and the resistance of the SAM (RSAM)), unlike DC methods, independently of each other. We found that the resistance of the top-electrode-SAM contact for junctions with the form of AgTS-SCn//GaOx/EGaIn with n = 10, 12, 14, 16 or 18 is bias and temperature independent and hence Ohmic (non-rectifying) in nature, and is orders of magnitude smaller than RSAM. The CSAM and RSAM are independent of the temperature, indicating that the mechanism of charge transport in these SAM-based junctions is coherent tunneling and the charge carrier trapping at the interfaces is negligible. Electronic supplementary information (ESI) available: Detailed experimental procedure, Nyquist

  9. The Search for Nitrates on Mars by the Sample Analysis at Mars (SAM) Instrument

    NASA Technical Reports Server (NTRS)

    Navarro-Gonzalez, Rafael; Stern, Jennifer C.; Freissinet, Caroline; McKay, Chirstopher P.; Sutter, Brad; Archer, P. Douglas, Jr.; McAdam, Amy; Franz, Heather; Coll, Partice J.; Glavin, Daniel Patrick; Eigenbrode, Jennifer L.; Wong, Mike; Atreya, Sushiil K.; Wray, James J.; Steele, Andrew; Prats, Benito D.; Szopa, Cyril; Coscia, David; Teinturier, Samuel; Buch, Arnaud; Leshin, Laurie A.; Ming, Douglas W.; Conrad, Pamela Gales; Cabane, Michel; Mahaffy, Paul R.; Grotzinger, John P.

    2013-01-01

    Planetary models suggest that nitrogen was abundant in the early Martian atmosphere as N2 but it was lost by sputtering and photochemical loss to space, impact erosion, and chemical oxidation to nitrates. A nitrogen cycle may exist on Mars where nitrates, produced early in Mars' history, may have been later decomposed back into N2 by the current impact flux. Nitrates are a fundamental source of nitrogen for terrestrial microorganisms, and they have evolved metabolic pathways to perform both oxidation and reduction to drive a complete biological nitrogen cycle. Therefore, the characterization of nitrogen in Martian soils is important to assess habitability of the Martian environment, particularly with respect to the presence of nitrates. The only previous mission that was designed to search for soil nitrates was the Phoenix mission but N-containing species were not detected by TEGA or the MECA WCL. Nitrates have been tentatively identified in Nakhla meteorites, and if nitrogen was oxidized on Mars, this has important implications for the habitability potential of Mars. Here we report the results from the Sample Analysis at Mars (SAM) instrument suite aboard the Curiosity rover during the first year of surface operations in Gale Crater. Samples from the Rocknest aeolian deposit and sedimentary rocks (John Klein) were heated to approx 835degC under helium flow and the evolved gases were analyzed by MS and GC-MS. Two and possibly three peaks may be associated with the release of m/z 30 at temperatures ranging from 180degC to 500degC. M/z 30 has been tentatively identified as NO; other plausible contributions include CH2O and an isotopologue of CO, 12C18O. NO, CH2O, and CO may be reaction products of reagents (MTBSTFA/DMF) carried from Earth for the wet chemical derivatization experiments with SAM and/or derived from indigenous soil nitrogenated organics. Laboratory analyses indicate that it is also possible that <550degC evolved NO is produced via reaction of HCl with

  10. Fluorocarbon Contamination from the Drill on the Mars Science Laboratory: Potential Science Impact on Detecting Martian Organics by Sample Analysis at Mars (SAM)

    NASA Technical Reports Server (NTRS)

    Eigenbrode, J. L.; McAdam, A.; Franz, H.; Freissinet, C.; Bower, H.; Floyd, M.; Conrad, P.; Mahaffy, P.; Feldman, J.; Hurowitz, J.; Evans, J.; Anderson, M.; Jandura, L.; Brown, K.; Logan, C.; Kuhn, S.; Anderson, R.; Beegle, L.; Limonadi, D.; Rainen, R.; Umland, J.

    2013-01-01

    Polytetrafluoroethylene (PTFE or trade name: Teflon by Dupont Co.) has been detected in rocks drilled during terrestrial testing of the Mars Science Laboratory (MSL) drilling hardware. The PTFE in sediments is a wear product of the seals used in the Drill Bit Assemblies (DBAs). It is expected that the drill assembly on the MSL flight model will also shed Teflon particles into drilled samples. One of the primary goals of the Sample Analysis at Mars (SAM) instrument suite on MSL is to test for the presence of martian organics in samples. Complications introduced by the potential presence of PTFE in drilled samples to the SAM evolved gas analysis (EGA or pyrolysisquadrupole mass spectrometry, pyr-QMS) and pyrolysis- gas chromatography mass spectrometry (Pyr- GCMS) experiments was investigated.

  11. New insight into the electrochemical desorption of alkanethiol SAMs on gold.

    PubMed

    Pensa, Evangelina; Vericat, Carolina; Grumelli, Doris; Salvarezza, Roberto C; Park, Sung Hyun; Longo, Gabriel S; Szleifer, Igal; Méndez De Leo, Lucila P

    2012-09-21

    A combination of Polarization Modulation Infrared Reflection Absorption Spectroscopy (PMIRRAS) under electrochemical control, Electrochemical Scanning Tunneling Microscopy (ECSTM) and Molecular Dynamics (MD) simulations has been used to shed light on the reductive desorption process of dodecanethiol (C12) and octadecanethiol (C18) SAMs on gold in aqueous electrolytes. Experimental PMIRRAS, ECSTM and MD simulations data for C12 desorption are consistent with formation of randomly distributed micellar aggregates stabilized by Na(+) ions, coexisting with a lying-down phase of molecules. The analysis of pit and Au island coverage before and after desorption is consistent with the thiolate-Au adatoms models. On the other hand, PMIRRAS and MD data for C18 indicate that the desorbed alkanethiolates adopt a Na(+) ion-stabilized bilayer of interdigitated alkanethiolates, with no evidence of lying down molecules. MD simulations also show that both the degree of order and tilt angle of the desorbed alkanethiolates change with the surface charge on the metal, going from bilayers to micelles. These results demonstrate the complexity of the alkanethiol desorption in the presence of water and the fact that chain length and counterions play a key role in a complex structure.

  12. Possible Detection of Nitrates on Mars by the Sample Analysis at Mars (SAM) Instrument

    NASA Technical Reports Server (NTRS)

    Navarro-Gonzalez, R.; Stern, J.; Sutter, B.; Archer, D.; McAdam, A.; Franz, H. B.; McKay, C. P.; Coll, P.; Cabane, M.; Ming, D. W.; Brunner, A. E.; Glavin, D.; Eigenbrode, J. L.; Jones, J. H.; Freissinet, C.; Leshin, L.; Wong, M.; Atreya, S.; Wray, J. J.; Steele, A.; Buch, A.; Prats, B. D.; Szopa, C.; Conrad, P.; Mahaffy, P.

    2013-01-01

    Planetary models suggest that nitrogen was abundant in the early Martian atmosphere as dinitrogen (N2). However, it has been lost by sputtering and photochemical loss to space [1, 2], impact erosion [3], and chemical oxidation to nitrates [4]. Nitrates, produced early in Mars history, are later decomposed back into N2 by the current impact flux [5], making possible a nitrogen cycle on Mars. It is estimated that a layer of about 3 m of pure NaNO3 should be distributed globally on Mars [5]. Nitrates are a fundamental source for nitrogen to terrestrial microorganisms. Therefore, the detection of soil nitrates is important to assess habitability in the Martian environment. The only previous mission that was designed to search for soil nitrates was the Phoenix mission but was unable to detect evolved N-containing species by TEGA and the MECA WCL [6]. Nitrates have been tentatively identified in the Nakhla meteorite [7]. The purpose of this work is to determine if nitrates were detected in first solid sample (Rocknest) in Gale Crater examined by the SAM instrument.

  13. Carbon-sulfur bond-forming reaction catalysed by the radical SAM enzyme HydE

    NASA Astrophysics Data System (ADS)

    Rohac, Roman; Amara, Patricia; Benjdia, Alhosna; Martin, Lydie; Ruffié, Pauline; Favier, Adrien; Berteau, Olivier; Mouesca, Jean-Marie; Fontecilla-Camps, Juan C.; Nicolet, Yvain

    2016-05-01

    Carbon-sulfur bond formation at aliphatic positions is a challenging reaction that is performed efficiently by radical S-adenosyl-L-methionine (SAM) enzymes. Here we report that 1,3-thiazolidines can act as ligands and substrates for the radical SAM enzyme HydE, which is involved in the assembly of the active site of [FeFe]-hydrogenase. Using X-ray crystallography, in vitro assays and NMR spectroscopy we identified a radical-based reaction mechanism that is best described as the formation of a C-centred radical that concomitantly attacks the sulfur atom of a thioether. To the best of our knowledge, this is the first example of a radical SAM enzyme that reacts directly on a sulfur atom instead of abstracting a hydrogen atom. Using theoretical calculations based on our high-resolution structures we followed the evolution of the electronic structure from SAM through to the formation of S-adenosyl-L-cysteine. Our results suggest that, at least in this case, the widely proposed and highly reactive 5‧-deoxyadenosyl radical species that triggers the reaction in radical SAM enzymes is not an isolable intermediate.

  14. Expression and functions of the star proteins Sam68 and T-STAR in mammalian spermatogenesis.

    PubMed

    Ehrmann, Ingrid; Elliott, David J

    2010-01-01

    Spermatogenesis is one of the few major developmental pathways which are still ongoing in the adult. In this chapter we review the properties of Sam68 and T-STAR, which are the STAR proteins functionally implicated in mammalian spermatogenesis. Sam68 is a ubiquitously expressed member of the STAR family, but has an essential role in spermatogenesis. Sam68 null mice are male infertile and at least in part this is due to a failure in important translational controls that operate during and after meiosis. The homologous T-STAR protein has a much more restricted anatomic expression pattern than Sam68, with highest levels seen in the testis and the developing brain. The focus of this chapter is the functional role of Sam68 and T-STAR proteins in male germ cell development. Since these proteins are known to have many cellular functions we extrapolate from other cell types and tissues to speculate on each of their likely functions within male germ cells, including control of alternative pre-mRNA splicing patterns in male germ cells.

  15. SAM-FS: LSC's New Solaris-Based Storage Management Product

    NASA Technical Reports Server (NTRS)

    Angell, Kent

    1996-01-01

    SAM-FS is a full featured hierarchical storage management (HSM) device that operates as a file system on Solaris-based machines. The SAM-FS file system provides the user with all of the standard UNIX system utilities and calls, and adds some new commands, i.e. archive, release, stage, sls, sfind, and a family of maintenance commands. The system also offers enhancements such as high performance virtual disk read and write, control of the disk through an extent array, and the ability to dynamically allocate block size. SAM-FS provides 'archive sets' which are groupings of data to be copied to secondary storage. In practice, as soon as a file is written to disk, SAM-FS will make copies onto secondary media. SAM-FS is a scalable storage management system. The system can manage millions of files per system, though this is limited today by the speed of UNIX and its utilities. In the future, a new search algorithm will be implemented that will remove logical and performance restrictions on the number of files managed.

  16. Study of SAM surface morphology, integrity and film quality on various Au surfaces

    NASA Astrophysics Data System (ADS)

    Murphy, John; Senevirathne, Indrajith

    2013-03-01

    SAM (Self Assembled Monolayer) surfaces have many possible applications from polymer based electronics to sensor engineering. Common substrate architecture for such systems happens to be Au(111) on mica. Au on layered mica lacks mechanical resilience towards engineering applications. Solutions of 1-dodecanethiol (hydrophobic -R), dissolved in 200 proof Ethanol with 5mM concentration were prepared. These solutions were used in developing SAMs on purchased, clean flat Au(111) on mica (standard), Au sputter deposited on mica, Hydrogen flame annealed Au layers on glass, and Hydrogen flame annealed Au layers on mica. Resulting SAM surfaces were investigated with regular and custom built hydrophilic and hydrophobic AFM (Atomic Force Microcopy) probes via contact, and non contact AFM with topography and phase imaging. Surface integrity, roughness, corrugation and morphology on SAM surfaces were estimated. Preliminary data indicated total RMS surface roughness at ~ 2.8nm for SAMs on typical gold surfaces on mica purchased (standard) while varying RMS surface roughness estimates on subsequent surfaces with flame annealed samples showing similar RMS surface roughness. LHU Nanotechnology Program, PASSHE FPDC (LOU # 2010-LHU-03).

  17. Characterization of a S-adenosyl-l-methionine (SAM)-accumulating strain of Scheffersomyces stipitis.

    PubMed

    Križanović, Stela; Butorac, Ana; Mrvčić, Jasna; Krpan, Maja; Cindrić, Mario; Bačun-Družina, Višnja; Stanzer, Damir

    2015-06-01

    S-adenosyl-l-methionine (SAM) is an important molecule in the cellular metabolism of mammals. In this study, we examined several of the physiological characteristics of a SAM-accumulating strain of the yeast Scheffersomyces stipitis (M12), including SAM production, ergosterol content, and ethanol tolerance. S. stipitis M12 accumulated up to 52.48 mg SAM/g dry cell weight. Proteome analyses showed that the disruption of C-24 methylation in ergosterol biosynthesis, a step mediated by C-24 sterol methyltransferase (Erg6p), results in greater SAM accumulation by S. stipitis M12 compared to the wild-type strain. A comparative proteome-wide analysis identified 25 proteins that were differentially expressed by S. stipitis M12. These proteins are involved in ribosome biogenesis, translation, the stress response, ubiquitin-dependent catabolic processes, the cell cycle, ethanol tolerance, posttranslational modification, peroxisomal membrane stability, epigenetic regulation, the actin cytoskeleton and cell morphology, iron and copper homeostasis, cell signaling, and energy metabolism.

  18. The relative influence of ENSO and SAM on Antarctic Peninsula climate

    NASA Astrophysics Data System (ADS)

    Clem, Kyle R.; Renwick, James A.; McGregor, James; Fogt, Ryan L.

    2016-08-01

    Recent warming of the Antarctic Peninsula during austral autumn, winter, and spring has been linked to sea surface temperature (SST) trends in the tropical Pacific and tropical Atlantic, while warming of the northeast Peninsula during summer has been linked to a strengthening of westerly winds traversing the Peninsula associated with a positive trend in the Southern Annular Mode (SAM). Here we demonstrate that circulation changes associated with the SAM dominate interannual temperature variability across the entire Antarctic Peninsula during both summer and autumn, while relationships with tropical Pacific SST variability associated with the El Niño-Southern Oscillation (ENSO) are strongest and statistically significant primarily during winter and spring only. We find the ENSO-Peninsula temperature relationship during autumn to be weak on interannual time scales and regional circulation anomalies associated with the SAM more important for interannual temperature variability across the Peninsula during autumn. Consistent with previous studies, western Peninsula temperatures during autumn, winter, and spring are closely tied to changes in the Amundsen Sea Low (ASL) and associated meridional wind anomalies. The interannual variability of ASL depth is most strongly correlated with the SAM index during autumn, while the ENSO relationship is strongest during winter and spring. Investigation of western and northeast Peninsula temperatures separately reveals that interannual variability of northeast Peninsula temperatures is primarily sensitive to zonal wind anomalies crossing the Peninsula and resultant leeside adiabatic warming rather than to meridional wind anomalies, which is closely tied to variability in the zonal portion of the SAM pattern.

  19. Structural and functional comparison of HemN to other radical SAM enzymes.

    PubMed

    Layer, Gunhild; Kervio, Eric; Morlock, Gaby; Heinz, Dirk W; Jahn, Dieter; Retey, Janos; Schubert, Wolf-Dieter

    2005-10-01

    Radical SAM enzymes have only recently been recognized as an ancient family sharing an unusual radical-based reaction mechanism. This late appreciation is due to the extreme oxygen sensitivity of most radical SAM enzymes, making their characterization particularly arduous. Nevertheless, realization that the novel apposition of the established cofactors S-adenosylmethionine and [4Fe-4S] cluster creates an explosive source of catalytic radicals, the appreciation of the sheer size of this previously neglected family, and the rapid succession of three successfully solved crystal structures within a year have ensured that this family has belatedly been noted. In this review, we report the characterization of two enzymes: the established radical SAM enzyme, HemN or oxygen-independent coproporphyrinogen III oxidase from Escherichia coli, and littorine mutase, a presumed radical SAM enzyme, responsible for the conversion of littorine to hyoscyamine in plants. The enzymes are compared to other radical SAM enzymes and in particular the three reported crystal structures from this family, HemN, biotin synthase and MoaA, are discussed.

  20. 77 FR 13295 - Subcommittee Meeting of the Board of Advisors to the President of the Naval War College Subcommittee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-06

    ... Committee is: Board of Advisors (BOA) to the Presidents of the Naval Postgraduate School and the Naval War..., information, or to send written comments regarding the NWC BOA Subcommittee contact Mr. Richard R....

  1. 75 FR 53958 - Meeting of the Board of Advisors to the Presidents of the Naval Postgraduate School and the Naval...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-02

    ...), notice is hereby given that the following meeting of the Board of Advisors (BOA) to the Presidents of the... BOA, contact Ms. Jaye Panza, Naval Postgraduate School, 1 University Circle, Monterey, CA...

  2. The role of community health advisors in community-based participatory research.

    PubMed

    Story, Lachel; Hinton, Agnes; Wyatt, Sharon B

    2010-01-01

    Mistrust and fear of research often exist in minority communities because of assumptions, preconceived ideas, and historical abuse and racism that continue to influence research participation. The research establishment is full of well-meaning 'outsider' investigators who recognize discrimination, health disparities, and insufficient health care providers in minority communities, but struggle in breaking through this history of mistrust. This article provides ethical insights from one such 'insider-outsider', community-based participatory research project implemented via community health advisors in the Mississippi Delta. Both community-based participatory research and community health advisors provide opportunities to address the ethical issues of trust, non-maleficence, and justice in minority communities. Implications for ethics-driven nursing research are discussed.

  3. U.K. Chief Scientific Advisor Discusses Funding, Priorities, and Communication

    NASA Astrophysics Data System (ADS)

    Tretkoff, Ernie

    2011-05-01

    Like other countries facing a tough economy, the government in the United Kingdom has been under pressure to make cutbacks; as budgets are becoming increasingly tighter, funding for scientific research competes with other priorities. However, in its most recent spending review, which came out last fall and sets spending levels for the next 4 years, the U.K. government provided flat funding levels for scientific research, while most government departments saw their budgets cut by 30-40%. “That was actually a major commitment by a government that was cutting everything,” Sir John Beddington, U.K. chief scientific advisor, told Eos in a one-on-one telephone interview in mid-April. Beddington, who has been chief scientific advisor since 2008, shared with Eos his thoughts on priorities for science in the United Kingdom in a tough budget environment, getting expert science advice for policy making, providing scientific information following disasters, and communicating about climate change.

  4. Detection of Organics at Mars: How Wet Chemistry Onboard SAM Helps

    NASA Technical Reports Server (NTRS)

    Buch, A.; Freissinet, Caroline; Szopa, C.; Glavin, D.; Coll, P.; Cabane, M.; Eigenbrode, J.; Navarro-Gonzalez, R.; Coscia, D.; Teinturier, S.; Mahaffy, P.

    2013-01-01

    For the first time in the history of space exploration, a mission of interest to astrobiology could be able to analyze refractory organic compounds in the soil of Mars. Wet chemistry experiment allow organic components to be altered in such a way that improves there detection either by releasing the compounds from sample matricies or by changing the chemical structure to be amenable to analytical conditions. The latter is particular important when polar compounds are present. Sample Analysis at Mars (SAM), on the Curiosity rover of the Mars Science Laboratory mission, has onboard two wet chemistry experiments: derivatization and thermochemolysis. Here we report on the nature of the MTBSTFA derivatization experiment on SAM, the detection of MTBSTFA in initial SAM results, and the implications of this detection.

  5. Distributed processing and analysis of physics data in the D0 SAM system at Fermilab

    SciTech Connect

    Igor V. Terekhov

    2001-08-30

    SAM (Sequential Access through Meta-data) is the data access system for the D0 high energy physics (HEP) experiment at Fermilab. The system is being developed and used to handle the Petabyte-scale experiment data. The D0 applications, like virtually all HEP applications, are data-intensive, which poses special problems for the data management and job control facilities in the distributed environment. The fundamental problem is to bring the user applications and the data together, and SAM attacks the problems from both sides. First, we describe how the system moves the data through the distributed disk cache. Second, we describe how SAM interacts with the batch system to synchronize parallel user jobs with the data availability. All the design solutions herein have been implemented in a real system that handles the mission-critical data of the D0 experiment; thus, we present our work from the standpoint of real experience.

  6. SPASM and Twitch Domains in S-Adenosylmethionine (SAM) Radical Enzymes*

    PubMed Central

    Grell, Tsehai A. J.; Goldman, Peter J.; Drennan, Catherine L.

    2015-01-01

    S-Adenosylmethionine (SAM, also known as AdoMet) radical enzymes use SAM and a [4Fe-4S] cluster to catalyze a diverse array of reactions. They adopt a partial triose-phosphate isomerase (TIM) barrel fold with N- and C-terminal extensions that tailor the structure of the enzyme to its specific function. One extension, termed a SPASM domain, binds two auxiliary [4Fe-4S] clusters and is present within peptide-modifying enzymes. The first structure of a SPASM-containing enzyme, anaerobic sulfatase-maturating enzyme (anSME), revealed unexpected similarities to two non-SPASM proteins, butirosin biosynthetic enzyme 2-deoxy-scyllo-inosamine dehydrogenase (BtrN) and molybdenum cofactor biosynthetic enzyme (MoaA). The latter two enzymes bind one auxiliary cluster and exhibit a partial SPASM motif, coined a Twitch domain. Here we review the structure and function of auxiliary cluster domains within the SAM radical enzyme superfamily. PMID:25477505

  7. The crystal structure of a novel SAM-dependent methyltransferase PH1915 from Pyrococcus horikoshii.

    SciTech Connect

    Sun, W.; Xu, X.; Pavlova, M.; Edwards, A.; Joachimiak, A.; Savchenko, A.; Christendat, D.; Biosciences Division; Univ. of Toronto; Univ. Health Network

    2005-01-01

    The S-adenosyl-L-methionine (SAM)-dependent methyltransferases represent a diverse and biologically important class of enzymes. These enzymes utilize the ubiquitous methyl donor SAM as a cofactor to methylate proteins, small molecules, lipids, and nucleic acids. Here we present the crystal structure of PH1915 from Pyrococcus horikoshii OT3, a predicted SAM-dependent methyltransferase. This protein belongs to the Cluster of Orthologous Group 1092, and the presented crystal structure is the first representative structure of this protein family. Based on sequence and 3D structure analysis, we have made valuable functional insights that will facilitate further studies for characterizing this group of proteins. Specifically, we propose that PH1915 and its orthologs are rRNA- or tRNA-specific methyltransferases.

  8. Bringing a Chemical Laboratory Named Sam to Mars on the 2011 Curiosity Rover

    NASA Technical Reports Server (NTRS)

    Mahaffy, P. R.; Bleacher, L.; Jones, A.; Conrad, P. G.; Cabane, M.; Webster, C. R.; Atreya, S. A.; Manning, H.

    2010-01-01

    An important goal of upcoming missions to Mars is to understand if life could have developed there. The task of the Sample Analysis at Mars (SAM) suite of instruments [1] and the other Curiosity investigations [2] is to move us steadily toward that goal with an assessment of the habitability of our neighboring planet through a series of chemical and geological measurements. SAM is designed to search for organic compounds and inorganic volatiles and measure isotope ratios. Other instruments on Curiosity will provide elemental analysis and identify minerals. SAM will analyze both atmospheric samples and gases evolved from powdered rocks that may have formed billions of years ago with Curiosity providing access to interesting sites scouted by orbiting cameras and spectrometers.

  9. Recovery Act update from Sr. Advisor Matt Rogers -- End of Obligations

    ScienceCinema

    Rogers, Matt

    2016-07-12

    Senior Advisor to the Secretary for Recovery Act Implementation Matt Rogers shares his thoughts as the Recovery Act reaches a critical milestone -- the end of the 2010 fiscal year and the last day to obligation contract and grant funding under the Recovery Act. For more information about the Recovery Act at the Department of Energy: http://www.energy.gov/recovery Follow the Department of Energy! http://facebook.com/energygov http://twitter.com/energy

  10. 78 FR 69155 - Altegris Advisors, L.L.C., et al.; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-18

    ... COMMISSION Altegris Advisors, L.L.C., et al.; Notice of Application November 12, 2013. AGENCY: Securities and...) and (B) of the Act, under sections 6(c) and 17(b) of the Act for an exemption from sections 17(a)(1) and (2) of the Act, and under section 6(c) of the Act for an exemption from rule 12d1- 2(a) under...

  11. Perceptions and experiences of using automated bolus advisors amongst people with type 1 diabetes: A longitudinal qualitative investigation

    PubMed Central

    Lawton, J.; Kirkham, J.; Rankin, D.; Barnard, K.; Cooper, C.L.; Taylor, C.; Heller, S.; Elliott, J.

    2014-01-01

    Aims We explored people's reasons for, and experiences of, using bolus advisors to determine insulin doses; and, their likes/dislikes of this technology. Subjects and methods 42 people with type 1 diabetes who had received instruction in use of bolus advisors during a structured education course were interviewed post-course and 6 months later. Data were analysed thematically. Results Participants who considered themselves to have poor mathematical skills highlighted a gratitude for, and heavy reliance on, advisors. Others liked and chose to use advisors because they saved time and effort calculating doses and/or had a data storage facility. Follow-up interviews highlighted that, by virtue of no longer calculating their doses, participants could become deskilled and increasingly dependent on advisors. Some forgot what their mealtime ratios were; others reported a misperception that, because they were pre-programmed during courses, these parameters never needed changing. Use of data storage facilities could hinder effective review of blood glucose data and some participants reported an adverse impact on glycaemic control. Discussion While participants liked and perceived benefits to using advisors, there may be unintended consequences to giving people access to this technology. To promote effective use, on-going input and education from trained health professionals may be necessary. PMID:25451897

  12. Expanding the Use of Time-Based Metering: Multi-Center Traffic Management Advisor

    NASA Technical Reports Server (NTRS)

    Landry, Steven J.; Farley, Todd; Hoang, Ty

    2005-01-01

    Time-based metering is an efficient air traffic management alternative to the more common practice of distance-based metering (or "miles-in-trail spacing"). Despite having demonstrated significant operational benefit to airspace users and service providers, time-based metering is used in the United States for arrivals to just nine airports and is not used at all for non-arrival traffic flows. The Multi-Center Traffic Management Advisor promises to bring time-based metering into the mainstream of air traffic management techniques. Not constrained to operate solely on arrival traffic, Multi-Center Traffic Management Advisor is flexible enough to work in highly congested or heavily partitioned airspace for any and all traffic flows in a region. This broader and more general application of time-based metering is expected to bring the operational benefits of time-based metering to a much wider pool of beneficiaries than is possible with existing technology. It also promises to facilitate more collaborative traffic management on a regional basis. This paper focuses on the operational concept of the Multi-Center Traffic Management Advisor, touching also on its system architecture, field test results, and prospects for near-term deployment to the United States National Airspace System.

  13. Application of artificial intelligence to melter control: Realtime process advisor for the scale melter facility

    SciTech Connect

    Edwards, Jr, R E

    1988-01-01

    The Defense Waste Processing Facility (DWPF) at the Savannah River Plant (SRP) is currently under construction and when completed will process high-level radioactive waste into a borosilicate glass wasteform. This facility will consist of numerous batch chemical processing steps as well as the continuous operation of a joule-heated melter and its off-gas treatment system. A realtime process advisor system based on Artificial Intelligence (AI) techniques has been developed and is currently in use at the semiworks facility, which is operating a 2/3 scale of the DWPF joule-heated melter. The melter advisor system interfaces to the existing data collection and control system and monitors current operations of this facility. The advisor then provides advice to operators and engineers when it identifies process problems. The current system is capable of identifying process problems such as feed system pluggages and thermocouple failures and providing recommended actions. The system also provides facilities normally with distributed control systems. These include the ability to display process flowsheets, monitor alarm conditions, and check the status of process interlocks. 7 figs.

  14. Data handling with SAM and art at the NOνA experiment

    NASA Astrophysics Data System (ADS)

    Aurisano, A.; Backhouse, C.; Davies, G. S.; Illingworth, R.; Mayer, N.; Mengel, M.; Norman, A.; Rocco, D.; Zirnstein, J.

    2015-12-01

    During operations, NOvA produces between 5,000 and 7,000 raw files per day with peaks in excess of 12,000. These files must be processed in several stages to produce fully calibrated and reconstructed analysis files. In addition, many simulated neutrino interactions must be produced and processed through the same stages as data. To accommodate the large volume of data and Monte Carlo, production must be possible both on the Fermilab grid and on off-site farms, such as the ones accessible through the Open Science Grid. To handle the challenge of cataloging these files and to facilitate their off-line processing, we have adopted the SAM system developed at Fermilab. SAM indexes files according to metadata, keeps track of each file's physical locations, provides dataset management facilities, and facilitates data transfer to off-site grids. To integrate SAM with Fermilab's art software framework and the NOvA production workflow, we have developed methods to embed metadata into our configuration files, art files, and standalone ROOT files. A module in the art framework propagates the embedded information from configuration files into art files, and from input art files to output art files, allowing us to maintain a complete processing history within our files. Embedding metadata in configuration files also allows configuration files indexed in SAM to be used as inputs to Monte Carlo production jobs. Further, SAM keeps track of the input files used to create each output file. Parentage information enables the construction of self-draining datasets which have become the primary production paradigm used at NOvA. In this paper we will present an overview of SAM at NOvA and how it has transformed the file production framework used by the experiment.

  15. Data handling with SAM and art at the NOvA experiment

    DOE PAGES

    Aurisano, A.; Backhouse, C.; Davies, G. S.; Illingworth, R.; Mayer, N.; Mengel, M.; Norman, A.; Rocco, D.; Zirnstein, J.

    2015-12-23

    During operations, NOvA produces between 5,000 and 7,000 raw files per day with peaks in excess of 12,000. These files must be processed in several stages to produce fully calibrated and reconstructed analysis files. In addition, many simulated neutrino interactions must be produced and processed through the same stages as data. To accommodate the large volume of data and Monte Carlo, production must be possible both on the Fermilab grid and on off-site farms, such as the ones accessible through the Open Science Grid. To handle the challenge of cataloging these files and to facilitate their off-line processing, we havemore » adopted the SAM system developed at Fermilab. SAM indexes files according to metadata, keeps track of each file's physical locations, provides dataset management facilities, and facilitates data transfer to off-site grids. To integrate SAM with Fermilab's art software framework and the NOvA production workflow, we have developed methods to embed metadata into our configuration files, art files, and standalone ROOT files. A module in the art framework propagates the embedded information from configuration files into art files, and from input art files to output art files, allowing us to maintain a complete processing history within our files. Embedding metadata in configuration files also allows configuration files indexed in SAM to be used as inputs to Monte Carlo production jobs. Further, SAM keeps track of the input files used to create each output file. Parentage information enables the construction of self-draining datasets which have become the primary production paradigm used at NOvA. In this study we will present an overview of SAM at NOvA and how it has transformed the file production framework used by the experiment.« less

  16. Results from the Curiosity Rover's SAM Investigation at Gale Crater and links to Habitability of Mars

    NASA Astrophysics Data System (ADS)

    Coll, P. J.; Mahaffy, P. R.; Webster, C. R.; Cabane, M.; Atreya, S. K.; Conrad, P. G.; Navarro-gonzalez, R.; SAM; MSL Science Teams

    2013-05-01

    The Mars Science Laboratory Mission (MSL) has as its goal an exploration of the potential of the ancient Gale Crater to support life. The Sample Analysis at Mars (SAM) instrument suite (3) contributes to this exploration of habitability with (1) a search for organic compounds in rocks and soils, (2) a determination of the composition of inorganic volatiles compounds in the atmosphere or extracted from solid materials, and (3) measurements of the isotopic composition of several of these volatiles. The initial exploration of the region near the landing point has revealed a diverse geology and SAM has made measurements of both atmospheric and solid samples. Additional prime exploration targets are the clay and sulfate layers in the central mound (Mt. Sharp) of Gale crater. SAM is located in the interior of the Curiosity rover. Nine other instruments complete the payload including an XRD/XRF instrument and a variety of imaging, laser induced breakdown spectroscopy, and elemental analysis instrumentation. Several of these instruments serve to locate sampling sites and interrogate candidate materials before solid sample is collected either with a drill or a scoop for delivery to SAM and the XRD/XRF instruments. SAM's instruments are a quadrupole mass spectrometer (QMS), a tunable laser spectrometer (TLS), and a 6-column gas chromatograph (GC) coupled through a solid sample transport system and a gas processing and enrichment system. SAM atmospheric runs include a determination of: new volume mixing ratios for the 5 major isotopic constituents; an upper limit for the volume mixing ratio of methane; C and O isotope ratios in CO2; D/H in water; and the 40Ar/36Ar ratio. Major evolved gases from fines scooped from an eolian drift were H2O, CO2, O2, SO2, and a number of minor species. Chlorine containing compounds in this material were tentatively identified as perchlorates.

  17. Searching for Reduced Carbon on the Surface of Mars: The SAM Combustion Experiment

    NASA Technical Reports Server (NTRS)

    Stern, J. C.; Malespin, C. A.; Mahaffy, P. R.; Webster, C. R.; Eigenbrode, J. L.; Archer, P. D., Jr.; Brunner, A. E.; Freissinet, C.; Franz, H. B.; Glavin, D. P.; Graham, H. V.; McAdam, A. C.; Ming, D. W.; Navarro-Gonzalez, R.; Niles, P. B.; Steele, A.; Sutter, B.; Trainer, M. G.

    2014-01-01

    The search for reduced carbon has been a major focus of past and present missions to Mars. Thermal evolved gas analysis was used by the Viking and Phoenix landers and is currently in use by the Sample Analysis at Mars (SAM) instrument suite on the Mars Science Laboratory (MSL) to characterize volatiles evolved from solid samples, including those associated with reduced organic species. SAM has the additional capability to perform a combustion experiment, in which a sample of Mars regolith is heated in the presence of oxygen and the composition of the evolved gases is measured using quadrupole mass spectrometry (QMS) and tunable laser spectrometry (TLS) [1]. Organics detection on the Martian surface has been complicated by oxidation and destruction during heating by soil oxidants [2], including oxychlorine compounds, and terrestrial organics in the SAM background contributed by one of the SAM wet chemistry reagents MTBSTFA (N-Methyl-N-tertbutyldimethylsilyl- trifluoroacetamide) [3,4]. Thermal Evolved Gas Analysis (TEGA) results from Phoenix show a mid temperature CO2 release between 400 C - 680 C speculated to be carbonate, CO2 adsorbed to grains, or combustion of organics by soil oxidants [5]. Low temperature CO2 evolutions (approx. 200 C - 400 C) were also present at all three sites in Gale Crater where SAM Evolved Gas Analysis (EGA) was performed, and potential sources include combustion of terrestrial organics from SAM, as well as combustion and/or decarboxylation either indigenous martian or exogenous organic carbon [4,6]. By performing an experiment to intentionally combust all reduced materials in the sample, we hope to compare the bulk abundance of CO2 and other oxidized species evolved by combustion to that evolved during an EGA experiment to estimate how much CO2 could be contributed by reduced carbon sources. In addition, C, O, and H isotopic compositions of CO2 and H2O measured by TLS can contribute information regarding the potential sources of these

  18. The survey of autobiographical memory (SAM): a novel measure of trait mnemonics in everyday life.

    PubMed

    Palombo, Daniela J; Williams, Lynne J; Abdi, Hervé; Levine, Brian

    2013-06-01

    Compared to the abundance of laboratory-based memory tasks, few measures exist to assess self-reported memory function. This need is particularly important for naturalistic mnemonic capacities, such as autobiographical memory (recall of events and facts from one's past), because it is difficult to reliably assess in the laboratory. Furthermore, naturalistic mnemonic capacities may show stable individual differences that evade the constraints of laboratory testing. The Survey of Autobiographical Memory (SAM) was designed to assess such trait mnemonics, or the dimensional characterization of self-reported mnemonic characteristics. The SAM comprises items assessing self-reported episodic autobiographical, semantic, and spatial memory, as well as future prospection. In a large sample of healthy young adults, the latent dimensional structure of the SAM was characterized with multiple correspondence analysis (MCA). This analysis revealed dimensions corresponding to general mnemonic abilities (i.e., good vs poor memory across subtypes), spatial memory, and future prospection. While episodic and semantic items did not separate in this data-driven analysis, these categories did show expected dissociations in relation to depression history and to laboratory-based measures of recollection. Remote spatial memory as assessed by the SAM showed the expected advantage for males over females. Spatial memory was also related to autobiographical memory performance. Brief versions of the SAM are provided for efficient research applications. Individual differences in memory function are likely related to other health-related factors, including personality, psychopathology, dementia risk, brain structure and function, and genotype. In conjunction with laboratory or performance based assessments, the SAM can provide a useful measure of naturalistic self-report trait mnemonics for probing these relationships. PMID:23063319

  19. Development of Fuzzy Logic and Neural Network Control and Advanced Emissions Modeling for Parallel Hybrid Vehicles

    SciTech Connect

    Rajagopalan, A.; Washington, G.; Rizzoni, G.; Guezennec, Y.

    2003-12-01

    This report describes the development of new control strategies and models for Hybrid Electric Vehicles (HEV) by the Ohio State University. The report indicates results from models created in NREL's ADvanced VehIcle SimulatOR (ADVISOR 3.2), and results of a scalable IC Engine model, called in Willan's Line technique, implemented in ADVISOR 3.2.

  20. The nucleation and growth of calcium oxalate monohydrate on self- assembled monolayers (SAMs)

    SciTech Connect

    Campbell, A.A.; Tarasevich, B.J.; Graff, G.L.; Fryxell, G.E.; Rieke, P.C.

    1992-05-01

    A physical chemical approach was used to study calcium oxalate monohydrate (COM) nucleation and growth on various organic interfaces. Self-assembling monolayers (SAMs), containing derivatized organic functional groups, were designed to mimic various amino acid residues present in both urine and stone matrix macromolecules. Derivatized surfaces include SAMs with terminal methyl, bromo, imidazole, and thiazolidine-carboxylic acid functional groups. Pronounced differences in COM deposition were observed for the various interfaces with the imidazole and thiazolidine surfaces having the greatest effect and the methyl and bromo groups having little or no nucleating potential.

  1. Mineral classification map using MF and SAM techniques: A case study in the Nohwa Island, Korea

    SciTech Connect

    Son, Young-Sun; Yoon, Wang-Jung

    2015-03-10

    The purpose of this study is to map pyprophyllite distribution at surface of the Nohwa deposit, Korea by using Advanced Spaceborne Thermal Emission and Reflectance Radiometer (ASTER) data. For this, combined Spectral Angle Mapper (SAM), and Matched Filtering (MF) technique based on mathematical algorithm was applied. The regional distribution of high-grade and low-grade pyrophyllite in the Nohwa deposit area could be differentiated by this method. The results of this study show that ASTER data analysis using combination of SAM and MF techniques will assist in exploration of pyrophyllite at the exposed surface.

  2. The Combustion Experiment on the Sample Analysis at Mars (SAM) Instrument Suite on the Curiosity Rover

    NASA Technical Reports Server (NTRS)

    Stern, J. C.; Malespin, C. A.; Eigenbrode, J.; Graham, H. V.; Archer, P. D.; Brunner, A.; Freissinet, C.; Franz, H. B.; Fuentes, J.; Glavin, D. P.; Mahaffy, P. R.; McAdam, A. C.; Ming, D. W.; Niles, P. B.; Steele, A.

    2014-01-01

    The combustion experiment on the Sample Analysis at Mars (SAM) suite on Curiosity will heat a sample of Mars regolith in the presence of oxygen and measure composition of the evolved gases using quadrupole mass spectrometry (QMS) and tunable laser spectrometry (TLS). QMS will enable detection of combustion products such as CO, CO2, NO, and other oxidized species, while TLS will enable precision measurements of the abundance and carbon isotopic composition (delta C-13) of the evolved CO2 and hydrogen isotopic composition (delta D) of H2O. SAM will perform a two-step combustion to isolate combustible materials below approx. 550 C and above approx. 550 C.

  3. In Situ Analysis of Mars Soil and Rocks Sample with the Sam Gcms Instrumentation Onboard Curiosity : Interpretation and Comparison of Measurements Done during the First Martian Year of Curiosity on Mars

    NASA Astrophysics Data System (ADS)

    Szopa, C.; Coll, P. J.; Cabane, M.; Buch, A.; Coscia, D.; Millan, M.; Francois, P.; Belmahadi, I.; Teinturier, S.; Navarro-Gonzalez, R.; Glavin, D. P.; Freissinet, C.; Steele, A.; Eigenbrode, J. L.; Mahaffy, P. R.

    2014-12-01

    The characterisation of the chemical and mineralogical composition of solid surface samples collected with the Curiosity rover is a primary objective of the SAM experiment. These data should provide essential clues on the past habitability of Gale crater. Amongst the SAM suite of instruments [1], SAM-GC (Gas Chromatograph) is devoted to identify and quantify volatiles evolved from the thermal (heating up to about 900°C)/chemical (derivatization procedure) treatment of any soil sample collected by the Curiosity rover. With the aim to search for potential organic molecules outgassed from the samples, SAM-GC analytical channels composed of thermal-desorption injector, and a MXT-CLP or a MXT-Q chromatographic column was chosen to achieve all the measurements done up today, with the aim to separate of a wide range of volatile inorganic and organic molecules. Four solid samples have been analyzed with GCMS, one sand sample collected at the Rocknest site, two rock samples (John Klein and Cumberland respectively) collected at the Yellowknife Bay site using the Curiosity driller, and one rock sample collected at the Kimberly site. All the measurements were successful and they produced complex chromatograms with both detectors used for SAM GC, i.e. a thermal conductivity detector and the SAM quandrupole mass spectrometer. Their interpretation already revealed the presence of an oxychlorine phase present in the sample which is at the origin of chlorohydrocarbons clearly identified [2] but this represents only a fraction of the GCMS signal recorded [3,4]. This work presents a systematic comparison of the GCMS measurements done for the different samples collected, supported by reference data obtained in laboratory with different spare models of the gas chromatograph, with the aim to bring new elements of interpretation of the SAM measurements. References: [1] Mahaffy, P. et al. (2012) Space Sci Rev, 170, 401-478. [2] Glavin, D. et al. (2013), JGR. [3] Leshin L. et al. (2013

  4. Analysis of the interactions between host factor Sam68 and viral elements during foot-and-mouth disease virus infection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The nuclear protein Src-associated protein of 68 kDa in mitosis (Sam68) is known to bind RNA and be involved in cellular processes triggered in response to environmental stresses, including virus infection. Interestingly, Sam68, is a multi-functional protein implicated in the life cycle of retroviru...

  5. Organic chemistry on surfaces: Direct cyclopropanation by dihalocarbene addition to vinyl terminated self-assembled monolayers (SAMs)

    PubMed Central

    Adamkiewicz, Malgorzata

    2014-01-01

    Summary C11-Vinyl-terminated self-assembled monolayers (SAMs) on silica surfaces are successfully modified in C–C bond forming reactions with dihalocarbenes to generate SAMs, terminated with dihalo- (fluoro, chloro, bromo) cyclopropane motifs with about 30% surface coverage. PMID:25550756

  6. Sam68 is tyrosine phosphorylated and recruited to signalling in peripheral blood mononuclear cells from HIV infected patients

    PubMed Central

    Najib, S; Rodríguez-Baño, J; Ríos, M J; Muniain, M A; Goberna, R; Sánchez-Margalet, V

    2005-01-01

    Human immunodeficiency virus (HIV) codes for a protein, Rev, that mediates the viral RNA export from the nucleus to the cytoplasm. Recently, it has been found that Sam68, the substrate of Src associated in mitosis, is a functional homologue of Rev, and a synergistic activator of Rev activity. Thus, it has been suggested that Sam68 may play an important role in the post-transcriptional regulation of HIV. Sam68 contains an RNA binding motif named KH [homology to the nuclear ribonucleoprotein (hnRNP) K]. Tyrosine phosphorylation of Sam68 and binding to SH3 domains have been found to negatively regulate its RNA binding capacity. Besides, tyrosine phosphorylation of Sam68 allows the formation of signalling complexes with other proteins containing SH2 and SH3 domains, suggesting a role in signal transduction of different systems in human lymphocytes, such as the T cell receptor, and leptin receptor, or the insulin receptor in other cell types. In the present work, we have found that Sam68 is tyrosine phosphorylated in peripheral blood mononuclear cells (PBMC) from HIV infected subjects, leading to the formation of signalling complexes with p85 the regulatory subunit of PI3K, GAP and STAT-3, and decreasing its RNA binding capacity. In contrast, PBMC from HIV infected subjects have lower expression levels of Sam68 compared with controls. These results suggest that Sam68 may play some role in the immune function of lymphocytes in HIV infection. PMID:16045742

  7. Estimation of head tissue-specific exposure from mobile phones based on measurements in the homogeneous SAM head.

    PubMed

    Gosselin, Marie-Christine; Kühn, Sven; Crespo-Valero, Pedro; Cherubini, Emilio; Zefferer, Marcel; Christ, Andreas; Kuster, Niels

    2011-09-01

    The maximum spatial peak exposure of each commercial mobile phone determined in compliance with the relevant safety and product standards is publicly available. However, this information is not sufficient for epidemiological studies aiming to correlate the use of mobile phones with specific cancers or to behavioral alterations, as the dominant location of the exposure may be anywhere in the head between the chin to above the ear, depending on the phone design. The objective of this study was to develop a methodology to determine tissue-specific exposure by expanding the post-processing of the measured surface or volume scans using standardized compliance testing equipment, that is, specific absorption rate (SAR) scanners. The transformation matrix was developed using the results from generic dipoles to evaluate the relation between the SAR in many brain regions of the Virtual Family anatomical phantoms and in virtual brain regions mapped onto the homogeneous SAM head. A set of transformation factors was derived to correlate the SAR induced in the SAM head to the SAR in the anatomical heads. The evaluation included the uncertainty associated with each factor, arising from the anatomical differences between the phantoms (typically less than 6 dB (4×)). The applicability of these factors was validated by performing simulations of four head models exposed to four realistic mobile phone models. The new methodology enables the reliable determination of the maximum and averaged exposure of specific tissues and functional brain regions to mobile phones when combined with mobile phone power control data, and therefore greatly strengthens epidemiological evaluations and improves information for the consumer.

  8. Estimation of head tissue-specific exposure from mobile phones based on measurements in the homogeneous SAM head.

    PubMed

    Gosselin, Marie-Christine; Kühn, Sven; Crespo-Valero, Pedro; Cherubini, Emilio; Zefferer, Marcel; Christ, Andreas; Kuster, Niels

    2011-09-01

    The maximum spatial peak exposure of each commercial mobile phone determined in compliance with the relevant safety and product standards is publicly available. However, this information is not sufficient for epidemiological studies aiming to correlate the use of mobile phones with specific cancers or to behavioral alterations, as the dominant location of the exposure may be anywhere in the head between the chin to above the ear, depending on the phone design. The objective of this study was to develop a methodology to determine tissue-specific exposure by expanding the post-processing of the measured surface or volume scans using standardized compliance testing equipment, that is, specific absorption rate (SAR) scanners. The transformation matrix was developed using the results from generic dipoles to evaluate the relation between the SAR in many brain regions of the Virtual Family anatomical phantoms and in virtual brain regions mapped onto the homogeneous SAM head. A set of transformation factors was derived to correlate the SAR induced in the SAM head to the SAR in the anatomical heads. The evaluation included the uncertainty associated with each factor, arising from the anatomical differences between the phantoms (typically less than 6 dB (4×)). The applicability of these factors was validated by performing simulations of four head models exposed to four realistic mobile phone models. The new methodology enables the reliable determination of the maximum and averaged exposure of specific tissues and functional brain regions to mobile phones when combined with mobile phone power control data, and therefore greatly strengthens epidemiological evaluations and improves information for the consumer. PMID:21416476

  9. 78 FR 3024 - Sam D. Hamilton Noxubee National Wildlife Refuge, MS; Intent To Prepare a Comprehensive...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-15

    ... of issues to consider in the planning process. DATES: To ensure consideration, we must receive your... determine how the public can use each refuge. The planning process is a way for us and the public to... our process for developing a CCP for Sam D. Hamilton Noxubee NWR in Mississippi. This notice...

  10. The transcription factor FBI-1 inhibits SAM68-mediated BCL-X alternative splicing and apoptosis

    PubMed Central

    Bielli, Pamela; Busà, Roberta; Di Stasi, Savino M; Munoz, Manuel J; Botti, Flavia; Kornblihtt, Alberto R; Sette, Claudio

    2014-01-01

    Alternative splicing (AS) is tightly coupled to transcription for the majority of human genes. However, how these two processes are linked is not well understood. Here, we unveil a direct role for the transcription factor FBI-1 in the regulation of AS. FBI-1 interacts with the splicing factor SAM68 and reduces its binding to BCL-X mRNA. This, in turn, results in the selection of the proximal 5′ splice site in BCL-X exon 2, thereby favoring the anti-apoptotic BCL-XL variant and counteracting SAM68-mediated apoptosis. Conversely, depletion of FBI-1, or expression of a SAM68 mutant lacking the FBI-1 binding region, restores the ability of SAM68 to induce BCL-XS splicing and apoptosis. FBI-1's role in splicing requires the activity of histone deacetylases, whose pharmacological inhibition recapitulates the effects of FBI-1 knockdown. Our study reveals an unexpected function for FBI-1 in splicing modulation with a direct impact on cell survival. PMID:24514149

  11. The transcription factor FBI-1 inhibits SAM68-mediated BCL-X alternative splicing and apoptosis.

    PubMed

    Bielli, Pamela; Busà, Roberta; Di Stasi, Savino M; Munoz, Manuel J; Botti, Flavia; Kornblihtt, Alberto R; Sette, Claudio

    2014-04-01

    Alternative splicing (AS) is tightly coupled to transcription for the majority of human genes. However, how these two processes are linked is not well understood. Here, we unveil a direct role for the transcription factor FBI-1 in the regulation of AS. FBI-1 interacts with the splicing factor SAM68 and reduces its binding to BCL-X mRNA. This, in turn, results in the selection of the proximal 5' splice site in BCL-X exon 2, thereby favoring the anti-apoptotic BCL-XL variant and counteracting SAM68-mediated apoptosis. Conversely, depletion of FBI-1, or expression of a SAM68 mutant lacking the FBI-1 binding region, restores the ability of SAM68 to induce BCL-XS splicing and apoptosis. FBI-1's role in splicing requires the activity of histone deacetylases, whose pharmacological inhibition recapitulates the effects of FBI-1 knockdown. Our study reveals an unexpected function for FBI-1 in splicing modulation with a direct impact on cell survival.

  12. Cross-Reactive Sensor Array for Metal Ion Sensing Based on Fluorescent SAMs

    PubMed Central

    Basabe-Desmonts, Lourdes; van der Baan, Frederieke; Zimmerman, Rebecca S.; Reinhoudt, David N.; Crego-Calama, Mercedes

    2007-01-01

    Fluorescent self assembled monolayers (SAMs) on glass were previously developed in our group as new sensing materials for metal ions. These fluorescent SAMs are comprised by fluorophores and small molecules sequentially deposited on a monolayer on glass. The preorganization provided by the surface avoids the need for complex receptor design, allowing for a combinatorial approach to sensing systems based on small molecules. Now we show the fabrication of an effective microarray for the screening of metal ions and the properties of the sensing SAMs. A collection of fluorescent sensing SAMs was generated by combinatorial methods and immobilized on the glass surfaces of a custom-made 140 well microtiter-plate. The resulting libraries are easily measured and show varied responses to a series cations such as Cu2+, Co2+, Pb2+, Ca2+ and Zn2+. These surfaces are not designed to complex selectively a unique analyte but rather they are intended to produce fingerprint type responses to a range of analytes by less specific interactions. The unselective responses of the library to the presence of different cations generate a characteristic pattern for each analyte, a “finger print” response.

  13. Skills Assessment Module (S.A.M.): A Unique & Practical Approach to the Assessment Process.

    ERIC Educational Resources Information Center

    Rosinek, Michele

    The Skills Assessment Module (SAM) is designed to assess special needs students' affective, cognitive, and manipulative strengths and weaknesses in relation to vocational skills required in various training programs within a school system. Initial sections outline procedures and techniques (including paper-pencil tests, hands-on skill modules and…

  14. Treating Obsessive-Compulsive Disorder and Schizophrenia: The Case of Sam

    ERIC Educational Resources Information Center

    Peasley-Miklus, Catherine; Massie, Elise; Baslett, Gaston; Carmin, Cheryl

    2005-01-01

    This article describes the case of Sam, a 22-year-old male with obsessive-compulsive disorder (OCD) and schizophrenia. The patient's background, the development and characteristics of his OCD and schizophrenia, and the history of what became a rather complicated treatment are described. In addition, four problem areas of therapy are identified.

  15. The formation of ACC and competition between polyamines and ethylene for SAM

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Ethylene biosynthesis involves the conversion of S-adenosylmethionine (SAM) to 1-aminocyclopropane-1-carboxylic acid (ACC) by ACC synthase (ACS). ACC is then converted to ethylene. The genes that encode enzymes in this pathway all belong to a family of genes. Differential transcriptional regulation ...

  16. 19. (4"X5" image enlarged from 2 1/4" negative) Sam Fowler, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. (4"X5" image enlarged from 2 1/4" negative) Sam Fowler, Photographer, February 1998 VIEW OF GEORGIA DOT BRIDGE NO. 051-00025D-01986N (JAMES P. HOULIHAN BRIDGE). NAVIGATIONAL LIGHT LOCATED ON TOP OF FENDER - Georgia DOT Bridge No. 051-00025D-01986N, US 17 & State Route 25 Spanning Savannah River, Port Wentworth, Chatham County, GA

  17. The Methodist Recruiting Officer Myth: Sir Sam Hughes and World War I Recruitment in French Canada.

    ERIC Educational Resources Information Center

    Morton, Desmond

    1986-01-01

    During World War I, so the story goes, recruiting in French Canada went slowly because the Minister of Militia, Sir Sam Hughes, was a bigoted Ontario Orangeman with the gall to appoint a Methodist minister as recruiting officer for Quebec. Secondary students analyze primary source materials regarding this matter. (RM)

  18. SamACO: variable sampling ant colony optimization algorithm for continuous optimization.

    PubMed

    Hu, Xiao-Min; Zhang, Jun; Chung, Henry Shu-Hung; Li, Yun; Liu, Ou

    2010-12-01

    An ant colony optimization (ACO) algorithm offers algorithmic techniques for optimization by simulating the foraging behavior of a group of ants to perform incremental solution constructions and to realize a pheromone laying-and-following mechanism. Although ACO is first designed for solving discrete (combinatorial) optimization problems, the ACO procedure is also applicable to continuous optimization. This paper presents a new way of extending ACO to solving continuous optimization problems by focusing on continuous variable sampling as a key to transforming ACO from discrete optimization to continuous optimization. The proposed SamACO algorithm consists of three major steps, i.e., the generation of candidate variable values for selection, the ants' solution construction, and the pheromone update process. The distinct characteristics of SamACO are the cooperation of a novel sampling method for discretizing the continuous search space and an efficient incremental solution construction method based on the sampled values. The performance of SamACO is tested using continuous numerical functions with unimodal and multimodal features. Compared with some state-of-the-art algorithms, including traditional ant-based algorithms and representative computational intelligence algorithms for continuous optimization, the performance of SamACO is seen competitive and promising.

  19. Arrays of high quality SAM-based junctions and their application in molecular diode based logic.

    PubMed

    Wan, Albert; Suchand Sangeeth, C S; Wang, Lejia; Yuan, Li; Jiang, Li; Nijhuis, Christian A

    2015-12-14

    This paper describes a method to fabricate a microfluidic top-electrode that can be utilized to generate arrays of self-assembled monolayer (SAM)-based junctions. The top-electrodes consist of a liquid-metal of GaOx/EGaIn mechanically stabilized in microchannels and through-holes in polydimethylsiloxane (PDMS); these top-electrodes form molecular junctions by directly placing them onto the SAM supported by template-stripped (TS) Ag or Au bottom-electrodes. Unlike conventional techniques to form multiple junctions, our method does not require lithography to pattern the bottom-electrode and is compatible with TS bottom-electrodes, which are ultra-flat with large grains, free from potential contamination of photoresist residues, and do not have electrode-edges where the molecules are unable to pack well. We formed tunneling junctions with n-alkanethiolate SAMs in yields of ∼80%, with good reproducibility and electrical stability. Temperature dependent J(V) measurements indicated that the mechanism of charge transport across the junction is coherent tunneling. To demonstrate the usefulness of these junctions, we formed molecular diodes based on SAMs with Fc head groups. These junctions rectify currents with a rectification ratio R of 45. These molecular diodes were incorporated in simple electronic circuitry to demonstrate molecular diode-based Boolean logic.

  20. Statement of Facts for 1977 City-Wide Mock Trial Competitions. Walker Thomas v. Sam Nomad.

    ERIC Educational Resources Information Center

    National Inst. for Citizen Education in the Law, Washington, DC.

    Prepared by the District of Columbia Street Law Project for its annual city-wide mock trial competition, this instructional handout provides material for a civil case over an automobile accident. Walker Thomas is suing Sam Nomad for damages that resulted from a collision, for which both parties blame the other. The handout clarifies the laws and…

  1. SAM-based Cell Transfer to Photopatterned Hydrogels for Microengineering Vascular-Like Structures

    PubMed Central

    Sadr, Nasser; Zhu, Mojun; Osaki, Tatsuya; Kakegawa, Takahiro; Yang, Yunzhi; Moretti, Matteo; Fukuda, Junji; Khademhosseini, Ali

    2011-01-01

    A major challenge in tissue engineering is to reproduce the native 3D microvascular architecture fundamental for in vivo functions. Current approaches still lack a network of perfusable vessels with native 3D structural organization. Here we present a new method combining self-assembled monolayer (SAM)-based cell transfer and gelatin methacrylate hydrogel photopatterning techniques for microengineering vascular structures. Human umbilical vein cell (HUVEC) transfer from oligopeptide SAM-coated surfaces to the hydrogel revealed two SAM desorption mechanisms: photoinduced and electrochemically triggered. The former, occurs concomitantly to hydrogel photocrosslinking, and resulted in efficient (>97%) monolayer transfer. The latter, prompted by additional potential application, preserved cell morphology and maintained high transfer efficiency of VE-cadherin positive monolayers over longer culture periods. This approach was also applied to transfer HUVECs to 3D geometrically defined vascular-like structures in hydrogels, which were then maintained in perfusion culture for 15 days. As a step toward more complex constructs, a cell-laden hydrogel layer was photopatterned around the endothelialized channel to mimic the vascular smooth muscle structure of distal arterioles. This study shows that the coupling of the SAM-based cell transfer and hydrogel photocrosslinking could potentially open up new avenues in engineering more complex, vascularized tissue constructs for regenerative medicine and tissue engineering applications. PMID:21802723

  2. North Slope, Alaska: A case study of Sedimentary AeroMagnetics (SAM)

    SciTech Connect

    McConnell, T.J.

    1995-12-31

    Sedimentary AeroMagnetics (SAM) is the application of very high resolution aeromagnetic data to the exploration for hydrocarbons in the sedimentary section of a basin. Basically SAM provides very high spatial resolution of very low amplitude (<1 nT) anomalies originating from structures within the weakly magnetic sedimentary column. These sedimentary anomalies are superimposed upon the conventional high amplitude magnetic anomalies created by the crystalline basement. These low intensity anomalies generated from within the sediments are related to changes in the magnetic composition of the rocks. This can occur when rocks of differing magnetic susceptibilities are juxtaposed across a fault, or at a stratigraphic truncation. Fluid flow along faults, or at a stratigraphic truncation. Fluid flow along faults and fracture zones can also produce magnetization effects which can allow direct delineament of the fault or fracture zone. Stratigraphic units of weakly magnetic shale and sandstone also create subtle plateau effects in the magnetic amplitude maps. SAM data is a result of combining improvements in aeromagnetic data acquisition technology with new ideas on the design of the survey parameters. SAM surveys are generally flown at 200 - 800 meter line spacing with a terrain clearance of 60 - 150 meters. Data processing techniques are used which preserve the broad band frequency spectrum of the acquired data thereby allowing interpretation of the separate and distinct anomaly sources of the sedimentary column and of the crystalline basement.

  3. Making Time for Instructional Leadership. Volume 1: The Evolution of the SAM Process

    ERIC Educational Resources Information Center

    Goldring, Ellen; Grissom, Jason A.; Neumerski, Christine M.; Murphy, Joseph; Blissett, Richard; Porter, Andy

    2015-01-01

    This three-volume report describes the "SAM (School Administration Manager) process," an approach that about 700 schools around the nation are using to direct more of principals' time and effort to improve teaching and learning in classrooms. Research has shown that a principal's instructional leadership is second only to teaching among…

  4. Structure-guided discovery of carboxy-SAM as a novel metabolite modulating tRNA function

    PubMed Central

    Kim, Jungwook; Xiao, Hui; Bonanno, Jeffrey B.; Kalyanaraman, Chakrapani; Brown, Shoshana; Tang, Xiangying; Al-Obaidi, Nawar F.; Patskovsky, Yury; Babbitt, Patricia C.; Jacobson, Matthew P.; Lee, Young-Sam; Almo, Steven C.

    2014-01-01

    Identifying novel metabolites and characterizing their biological functions are major challenges of the post-genomic era. X-ray crystallography can reveal unanticipated ligands which persist through purification and crystallization. These adventitious protein:ligand complexes provide insights into new activities, pathways and regulatory mechanisms. We describe a new metabolite, carboxy-S-adenosylmethionine (Cx-SAM), its biosynthetic pathway and its role in tRNA modification. The structure of CmoA, a member of the SAM-dependent methyltransferase superfamily, revealed a ligand in the catalytic site consistent with Cx-SAM. Mechanistic analyses demonstrated an unprecedented role for prephenate as the carboxyl donor and the involvement of a unique ylide intermediate as the carboxyl acceptor in the CmoA-mediated conversion of SAM to Cx-SAM. A second member of the SAM-dependent methyltransferase superfamily, CmoB, recognizes Cx-SAM and acts as a carboxymethyltransferase to convert 5-hydroxyuridine (ho5U) into 5-oxyacetyl uridine (cmo5U) at the wobble position of multiple tRNAs in Gram negative bacteria1, resulting in expanded codon-recognition properties2,3. CmoA and CmoB represent the first documented synthase and transferase for Cx-SAM. These findings reveal new functional diversity in the SAM-dependent methyltransferase superfamily and expand the metabolic and biological contributions of SAM-based biochemistry. These discoveries highlight the value of structural genomics approaches for identifying ligands in the context of their physiologically relevant macromolecular binding partners and for aiding in functional assignment. PMID:23676670

  5. Mechanism elucidation of the radical SAM enzyme spore photoproduct lyase (SPL)

    PubMed Central

    Li, Lei

    2011-01-01

    Spore photoproduct lyase (SPL) repairs a special thymine dimer 5-thyminyl-5,6-dihydrothymine, which is commonly called spore photoproduct or SP at the bacterial early germination phase. SP is the exclusive DNA photo-damage product in bacterial endospores; its generation and swift repair by SPL are responsible for the spores’ extremely high UV resistance. The early in vivo studies suggested that SPL utilizes a direct reversal strategy to repair the SP in the absence of light. The research in the past decade further established SPL as a radical SAM enzyme, which utilizes a tri-cysteine CXXXCXXC motif to harbor a [4Fe-4S] cluster. At the 1+ oxidation state, the cluster provides an electron to the S-adenosylmethionine (SAM), which binds to the cluster in a bidentate manner as the fourth and fifth ligands, to reductively cleave the C-S bond associated with the sulfonium ion in SAM, generating a reactive 5′-deoxyadenosyl (5′-dA) radical. This 5′-dA radical abstracts the proR hydrogen atom from the C6 carbon of SP to initiate the repair process; the resulting SP radical subsequently fragments to generate a putative thymine methyl radical, which accepts a back-donated H atom to yield the repaired TpT. SAM is suggested to be regenerated at the end of each catalytic cycle; and only a catalytic amount of SAM is needed in the SPL reaction. The H atom source for the back donation step is suggested to be a cysteine residue (C141 in B. subtilis SPL), and the H-atom transfer reaction leaves a thiyl radical behind on the protein. This thiyl radical thus must participate in the SAM regeneration process; however how the thiyl radical abstracts an H atom from the 5′-dA to regenerate SAM is unknown. This paper reviews and discusses the history and the latest progress in the mechanistic elucidation of SPL. Despite some recent breakthroughs, more questions are raised in the mechanistic understanding of this intriguing DNA repair enzyme. PMID:22197590

  6. Analysis of chlorocarbon compounds identified in the SAM Investigation of the Mars Science Laboratory mission

    NASA Astrophysics Data System (ADS)

    Freissinet, Caroline; Mahaffy, P.; Glavin, D.; Buch, A.; Brunner, A.; Eigenbrode, J.; Martin, M.; Miller, K.; Steele, A.; Szopa, C.; SAM; MSL science Team

    2013-10-01

    The gas chromatograph mass spectrometer (GCMS) mode of the Sample Analysis at Mars (SAM) experiment was designed for the separation and identification of the chemical components of the gases released from a solid sample or trapped from the atmosphere. Gases from solid samples are either produced by heating a cell from ambient to >800-1100oC (EGA mode) or by wet chemistry extraction and reactions (not yet employed on Mars). Prior to EGA analysis of portions of the first 3 solid samples (Rocknest, John Klein and Cumberland) collected by MSL and delivered to SAM, an internal SAM blank run was carried out with an empty quartz cup. These blank analyses are required to understand the background signal intrinsic to the GCMS and its gas manifolds and traps. Several peaks have been identified as part of SAM background, some of them below the nmol level, which attests of the sensitivity of the instrument and as-designed performance of the GCMS. The origin of each peak has been investigated, and two major contributors are revealed; residual vapor from one of the chemicals used for SAM wet chemistry experiment: N-methyl-N-tert-butyldimethylsilyl-trifluoroacetamide (MTBSTFA), and the Tenax from the hydrocarbon trap. Supporting lab experiments are in progress to understand the reaction pathways of the molecules identified in the SAM background. These experiments help elucidate which molecules may be interpreted as indigenous to Mars. Of the three solid samples analyzed on 11 runs, it was possible to detect and identify several chlorinated compounds including several chlorohydrocarbons. The chlorine is likely derived from the decomposition of martian perchlorates or other indigenous Cl-containing species while the origin of the carbon is presently under investigation for each detected molecule. To date, a subset these molecules have been identified in lab studies and a terrestrial contribution to the observed products are more easily explained. The combined results from SAM and

  7. A Response to Sam McKegney's "Strategies for Ethical Engagement: An Open Letter Concerning Non-Native Scholars of Native Literatures"

    ERIC Educational Resources Information Center

    Appleford, Rob

    2009-01-01

    This article presents the author's response to Sam McKegney's "Strategies for Ethical Engagement: An Open Letter Concerning Non-Native Scholars of Native Literatures." In his response to Sam's diagnosis of the malaise currently afflicting non-Aboriginal critics of this literature, the author attempts to consider the "cure" Sam offers (albeit…

  8. Molecular cloning, characterization and expression analysis of the SAMS gene during adventitious root development in IBA-induced tetraploid black locust.

    PubMed

    Quan, Jine; Zhang, Sheng; Zhang, Chunxia; Meng, Sen; Zhao, Zhong; Xu, Xuexuan

    2014-01-01

    S-Adenosylmethionine synthetase (SAMS) catalyzes the synthesis of S-adenosylmethionine (SAM), a precursor for ethylene and polyamine biosynthesis. Here, we report the isolation of the 1498 bp full-length cDNA sequence encoding tetraploid black locust (Robinia pseudoacacia L.) SAMS (TrbSAMS), which contains an open reading frame of 1179 bp encoding 392 amino acids. The amino acid sequence of TrbSAMS has more than 94% sequence identity to SAMSs from other plants, with a closer phylogenetic relationship to SAMSs from legumes than to SAMS from other plants. The TrbSAMS monomer consists of N-terminal, central, and C-terminal domains. Subcellular localization analysis revealed that the TrbSAMS protein localizes mainly to in the cell membrane and cytoplasm of onion epidermal cells and Arabidopsis mesophyll cell protoplasts. Indole-3-butyric acid (IBA)-treated cuttings showed higher levels of TrbSAMS transcript than untreated control cuttings during root primordium and adventitious root formation. TrbSAMS and its downstream genes showed differential expression in shoots, leaves, bark, and roots, with the highest expression observed in bark. IBA-treated cuttings also showed higher SAMS activity than control cuttings during root primordium and adventitious root formation. These results indicate that TrbSAMS might play an important role in the regulation of IBA-induced adventitious root development in tetraploid black locust cuttings. PMID:25285660

  9. Molecular Cloning, Characterization and Expression Analysis of the SAMS Gene during Adventitious Root Development in IBA-Induced Tetraploid Black Locust

    PubMed Central

    Quan, Jine; Zhang, Sheng; Zhang, Chunxia; Meng, Sen; Zhao, Zhong; Xu, Xuexuan

    2014-01-01

    S-Adenosylmethionine synthetase (SAMS) catalyzes the synthesis of S-adenosylmethionine (SAM), a precursor for ethylene and polyamine biosynthesis. Here, we report the isolation of the 1498 bp full-length cDNA sequence encoding tetraploid black locust (Robinia pseudoacacia L.) SAMS (TrbSAMS), which contains an open reading frame of 1179 bp encoding 392 amino acids. The amino acid sequence of TrbSAMS has more than 94% sequence identity to SAMSs from other plants, with a closer phylogenetic relationship to SAMSs from legumes than to SAMS from other plants. The TrbSAMS monomer consists of N-terminal, central, and C-terminal domains. Subcellular localization analysis revealed that the TrbSAMS protein localizes mainly to in the cell membrane and cytoplasm of onion epidermal cells and Arabidopsis mesophyll cell protoplasts. Indole-3-butyric acid (IBA)-treated cuttings showed higher levels of TrbSAMS transcript than untreated control cuttings during root primordium and adventitious root formation. TrbSAMS and its downstream genes showed differential expression in shoots, leaves, bark, and roots, with the highest expression observed in bark. IBA-treated cuttings also showed higher SAMS activity than control cuttings during root primordium and adventitious root formation. These results indicate that TrbSAMS might play an important role in the regulation of IBA-induced adventitious root development in tetraploid black locust cuttings. PMID:25285660

  10. Student goal orientation in learning inquiry skills with modifiable software advisors

    NASA Astrophysics Data System (ADS)

    Shimoda, Todd A.; White, Barbara Y.; Frederiksen, John R.

    2002-03-01

    A computer support environment (SCI-WISE) for learning and doing science inquiry projects was designed. SCI-WISE incorporates software advisors that give general advice about a skill such as hypothesizing. By giving general advice (rather than step-by-step procedures), the system is intended to help students conduct experiments that are more epistemologically authentic. Also, students using SCI-WISE can select the type of advice the advisors give and when they give advice, as well as modify the advisors' knowledge bases. The system is based partly on a theoretical framework of levels of agency and goal orientation. This framework assumes that giving students higher levels of agency facilitates higher-level goal orientations (such as mastery or knowledge building as opposed to task completion) that in turn produce higher levels of competence. A study of sixth grade science students was conducted. Students took a pretest questionnaire that measured their goal orientations for science projects and their inquiry skills. The students worked in pairs on an open-ended inquiry project that requires complex reasoning about human memory. The students used one of two versions of SCI-WISE - one that was modifiable and one that was not. After finishing the project, the students took a posttest questionnaire similar to the pretest, and evaluated the version of the system they used. The main results showed that (a) there was no correlation of goal orientation with grade point average, (b) knowledge-oriented students using the modifiable version tended to rate SCI-WISE more helpful than task-oriented students, and (c) knowledge-oriented pairs using the nonmodifiable version tended to have higher posttest inquiry skills scores than other pair types.

  11. Community health advisors as research partners: an evaluation of the training and activities.

    PubMed

    Johnson, Rhoda E; Green, B Lee; Anderson-Lewis, Charkarra; Wynn, Theresa A

    2005-01-01

    The feasibility of training large numbers of community health advisors as research partners (CHARPs) was evaluated using talking circles data and cancer activity questionnaires and logs. The talking circles data indicated that the CHARPs (n=108) valued their training and believed they learned necessary research partner skills. A review of contacts (n=7,956) provided evidence that CHARPs (n=883) could work as a team to deliver a variety of services over time to the community. The findings suggested that implementing a large scale intervention with CHARPs has the potential to increase the dissemination of cancer information and to reduce cancer disparities. PMID:15625505

  12. Perspective of the Science Advisor to the Waste Isolation Pilot Plant

    SciTech Connect

    WEART,WENDELL D.

    1999-09-03

    In 1975 Sandia National Laboratories (SNL) was asked by the predecessor to the Department of Energy to assume responsibility for the scientific programs necessary to assure the safe and satisfactory development of a geologic repository in the salt beds of southeast New Mexico. Sandia has continued in the role of Science Advisor to the Waste Isolation Pilot Plant (WIPP) to the present time. This paper will share the perspectives developed over the past 25 years as the project was brought to fruition with successful certification by the Environmental Protection Agency (EPA) on May 13, 1998 and commencement of operations on April 26, 1999.

  13. The load shedding advisor: An example of a crisis-response expert system

    NASA Technical Reports Server (NTRS)

    Bollinger, Terry B.; Lightner, Eric; Laverty, John; Ambrose, Edward

    1987-01-01

    A Prolog-based prototype expert system is described that was implemented by the Network Operations Branch of the NASA Goddard Space Flight Center. The purpose of the prototype was to test whether a small, inexpensive computer system could be used to host a load shedding advisor, a system which would monitor major physical environment parameters in a computer facility, then recommend appropriate operator reponses whenever a serious condition was detected. The resulting prototype performed significantly to efficiency gains achieved by replacing a purely rule-based design methodology with a hybrid approach that combined procedural, entity-relationship, and rule-based methods.

  14. Sam68/KHDRBS1 is critical for colon tumorigenesis by regulating genotoxic stress-induced NF-κB activation

    PubMed Central

    Fu, Kai; Sun, Xin; Wier, Eric M; Hodgson, Andrea; Liu, Yue; Sears, Cynthia L; Wan, Fengyi

    2016-01-01

    Nuclear factor kappa B (NF-κB)-mediated transcription is an important mediator for cellular responses to DNA damage. Genotoxic agents trigger a 'nuclear-to-cytoplasmic' NF-κB activation signaling pathway; however, the early nuclear signaling cascade linking DNA damage and NF-κB activation is poorly understood. Here we report that Src-associated-substrate-during-mitosis-of-68kDa/KH domain containing, RNA binding, signal transduction associated 1 (Sam68/KHDRBS1) is a key NF-κB regulator in genotoxic stress-initiated signaling pathway. Sam68 deficiency abolishes DNA damage-stimulated polymers of ADP-ribose (PAR) production and the PAR-dependent NF-κB transactivation of anti-apoptotic genes. Sam68 deleted cells are hypersensitive to genotoxicity caused by DNA damaging agents. Upregulated Sam68 coincides with elevated PAR production and NF-κB-mediated anti-apoptotic transcription in human and mouse colon cancer. Knockdown of Sam68 sensitizes human colon cancer cells to genotoxic stress-induced apoptosis and genetic deletion of Sam68 dampens colon tumor burden in mice. Together our data reveal a novel function of Sam68 in the genotoxic stress-initiated nuclear signaling, which is crucial for colon tumorigenesis. DOI: http://dx.doi.org/10.7554/eLife.15018.001 PMID:27458801

  15. Direct Patterning of Organic Self-Assembled Monolayer (SAM) on GaAs Surfaces via Dip-Pen Nanolithography (DPN)

    NASA Astrophysics Data System (ADS)

    Xiong, Peng; Keiper, Timothy; Wang, Xiaolei; Zhao, Jianhua

    2015-03-01

    Hybrid structures of functional molecules and solid-state (SS) materials have attracted extensive interest in surface nanoscience and molecular electronics. The formation and micro/nano patterning of organic SAMs on SS surfaces are a key step in fabricating such devices. Here we report realization of high quality MHA SAMs on GaAs and direct formation of micro/nanoscale patterns of MHA SAM on the surface by micro-contact printing (μ CP) and DPN. The process begins with the preparation of an oxide-free surface of GaAs, for which we employed treatment by an ammonium polysulfide ((NH4)2 Sx) solution. The treatment strips native oxides from GaAs creating an atomic layer of sulfur covalently bonded to the fresh surface. Formation of high-quality SAMs of thiol molecules on GaAs then proceeds through exchange of the sulfur and the thiol terminal of the molecules. The effects of the sulfur-passivation and formation of MHA SAM on the treated surface were confirmed by XPS, HRTEM, and DPN. To the best of our knowledge, this is a first realization of direct DPN of nanoscale organic SAM on a semiconductor free of surface oxide. We further evidence the utility of the hybrid platform by demonstrating directed self-assembly of Au nanoparticles onto MHA/ODT SAM templates on GaAs.

  16. Stability of phosphonic self assembled monolayers (SAMs) on cobalt chromium (Co-Cr) alloy under oxidative conditions

    NASA Astrophysics Data System (ADS)

    Bhure, Rahul; Abdel-Fattah, Tarek M.; Bonner, Carl; Hall, Felicia; Mahapatro, Anil

    2011-04-01

    Cobalt chromium (Co-Cr) alloys have been widely used in the biomedical arena for cardiovascular, orthopedic and dental applications. Surface modification of the alloy allows us to tailor the interfacial properties to address critical challenges of Co-Cr alloy in medical applications. Self assembled monolayers (SAMs) of Octadecylphosphonic acid (ODPA) have been used to form thin films on the oxide layer of the Co-Cr alloy surface by solution deposition technique. The SAMs formed were investigated for their stability to oxidative conditions of ambient laboratory environment over periods of 1, 3, 7 and 14 days. The samples were then characterized for their stability using X-ray Photoelectron Spectroscopy (XPS), Atomic Force Microscopy (AFM) and contact angle measurements. Detailed high energy XPS elemental scans confirmed the presence of the phosphonic monolayer after oxidative exposure which suggested that the SAMs were firmly attached to the oxide layer of Co-Cr alloy. AFM images gave topographical data of the surface and showed islands of SAMs on Co-Cr alloy surface, before and after SAM formation and also over the duration of the oxidative exposure. Contact angle measurements confirmed the hydrophobicity of the surface over 14 days. Thus the SAMs were found to be stable for the duration of the study. These SAMs could be subsequently tailored by modifying the terminal functional groups and could be used for various potential biomedical applications such as drug delivery, biocompatibility and tissue integration.

  17. Early Evolved Gas Results from the Curiosity Rover’s SAM Investigation at Gale Crater

    NASA Astrophysics Data System (ADS)

    Mahaffy, Paul R.; Franz, H.; McAdam, A.; Brunner, A.; Eigenbrode, J.; Stern, J.; SAM Science Team; MSL Science Team

    2013-10-01

    The Mars Science Laboratory Mission is designed to explore the habitability of the selected landing site at Gale crater. The Sample Analysis at Mars (SAM) instrument suite contributes to this study with a search for organic compounds, an analysis of the composition of inorganic volatiles, and measurements of the isotopic composition light elements. Both atmospheric and solid samples are analyzed. The layers in the central mound (Mt. Sharp) of Gale crater are important targets for the MSL mission. However, in situ measurements made during the past year of interesting regions close to the Bradbury landing site have revealed a diverse geology and several primary mission objectives have already been realized. SAM is located in the interior of the Curiosity rover. The MSL cameras, a laser induced breakdown spectrometer, and elemental analysis instrumentation serves to locate sampling sites and interogate candidate materials before solid sample is collected either with a drill or a scoop for delivery to SAM and the XRD instrument CheMin. SAM integrates a quadrupole mass spectrometer (QMS), a tunable laser spectrometer (TLS), and a 6-column gas chromatograph (GC) with a solid sample transport system and a gas processing and enrichment system. Results of SAM atmospheric composition analyses have already been reported (1,2). To date, multiple SAM evolved gas experiments have examined samples from fines scooped from an aeolian drift and from two drilled samples of a mudstone. Major evolved gases are H2O, CO2, O2, SO2, H2S, H2, and a number of minor species. These data help confirm the likely presence of perchlorates, the presence of phylosillicates, and both reduced and oxidized compounds evolved from the same sample. 1) P.R. Mahaffy et al., Abundance and Isotopic Composition of Gases in the Martian Atmosphere from the Curiosity Rover, Science 343, (2013). 2) C.R. Webster et al., Isotope Ratios of H, C and O in Martian Atmospheric Carbon Dioxide and Water Measured by the

  18. Early Results from the Curiosity Rover's SAM Investigation at Gale Crater

    NASA Astrophysics Data System (ADS)

    Mahaffy, Paul; Webster, Chris; Cabane, Michael; Coll, Patrice

    2013-04-01

    The goals of the Mars Science Laboratory Mission (1, 2) are to explore the potential of the Gale Crater landing site to support life either in the distant past or the present. The contribution of the Sample Analysis at Mars (SAM) instrument suite (3) in this exploration of habitability is (A) to search for organic compounds in rocks and soils, (B) to determine the composition of inorganic volatiles compounds in the atmosphere or extracted from solid materials, and (C) to measure the isotopic composition of several of these volatiles. While prime exploration targets of MSL's Curiosity Rover are the layers in the central mound (Mt. Sharp) of Gale crater the initial exploration of region near the landing point has revealed a diverse geology and the early part of the mission has been spent both commissioning the 10 Curiosity instruments and the Rover subsystems and making first time measurements of both atmospheric and solid samples. SAM is located in the interior of MSL's Curiosity rover next to the XRD/XRF CheMin instrument. A variety of imaging, laser induced breakdown spectroscopy, and elemental analysis instrumentation serves to locate sampling sites and interogate candidate materials before solid sample is collected either with a drill or a scoop for delivery to SAM and CheMin. SAM's instruments are a quadrupole mass spectrometer (QMS), a tunable laser spectrometer (TLS), and a 6-column gas chromatograph (GC). These are coupled through a solid sample transport system and a gas processing and enrichment system. The SAM suite is able to measure a suite of light isotopes and to analyze volatiles directly from the atmosphere or thermally released from solid samples. Early results from SAM atmospheric runs include a determination of: new volume mixing ratios for the 5 major isotopic constituents showing Ar approximately equal to N2; an upper limit of 3.5 ppb for the volume mixing ratio of methane; C and O isotope ratios showing both heavier than terrestrial averages

  19. Application of Neutron-Absorbing Structural-Amorphous Metal (SAM) Coatings for Spent Nuclear Fuel (SNF) Container to Enhance Criticality Safety Control

    SciTech Connect

    Choi, J

    2007-01-12

    This report describes the analysis and modeling approaches used in the evaluation for criticality-control applications of the neutron-absorbing structural-amorphous metal (SAM) coatings. The applications of boron-containing high-performance corrosion-resistant material (HPCRM)--amorphous metal as the neutron-absorbing coatings to the metallic support structure can enhance criticality safety controls for spent nuclear fuel in baskets inside storage containers, transportation casks, and disposal containers. The use of these advanced iron-based, corrosion-resistant materials to prevent nuclear criticality in transportation, aging, and disposal containers would be extremely beneficial to the nuclear waste management programs.

  20. Curiosity's Sample Analysis at Mars (SAM) Investigation: Overview of Results from the First 120 Sols on Mars

    NASA Technical Reports Server (NTRS)

    Mahaffy, P. R.; Cabane, M.; Webster, C. R.; Archer, P. D.; Atreya, S. K.; Benna, M.; Brinckerhoff, W. B.; Brunner, A. E.; Buch, A.; Coll, P.; Conrad, P. G.; Coscia, D.; Dobson, N.; Dworkin, J. P.; Eigenbrode, J. L.; Farley, K. A.; Flesch, G.; Franz, H. B.; Freissinet, C.; Gorevan, S.; Glavin, D. P.; Grotzinger, J. P.; Harpold, D. N.; Hengemihle, J.; Jaeger, F.

    2013-01-01

    During the first 120 sols of Curiosity s landed mission on Mars (8/6/2012 to 12/7/2012) SAM sampled the atmosphere 9 times and an eolian bedform named Rocknest 4 times. The atmospheric experiments utilized SAM s quadrupole mass spectrometer (QMS) and tunable laser spectrometer (TLS) while the solid sample experiments also utilized the gas chromatograph (GC). Although a number of core experiments were pre-programmed and stored in EEProm, a high level SAM scripting language enabled the team to optimize experiments based on prior runs.