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Sample records for aerial hyphal growth

  1. A cellulose synthase-like protein involved in hyphal tip growth and morphological differentiation in streptomyces.

    PubMed

    Xu, Hongbin; Chater, Keith F; Deng, Zixin; Tao, Meifeng

    2008-07-01

    Cellulose synthase and cellulose synthase-like proteins, responsible for synthesizing beta-glucan-containing polysaccharides, play a fundamental role in cellular architectures, such as plant cell and tissue morphogenesis, bacterial biofilm formation, and fruiting-body development. However, the roles of the proteins involved in the developmental process are not well understood. Here, we report that a cellulose synthase-like protein (CslA(Sc)) in Streptomyces has a function in hyphal tip growth and morphological differentiation. The cslA(Sc) replacement mutant showed pleiotropic defects, including the severe delay of aerial-hyphal formation and altered cell wall morphology. Calcofluor white fluorescence analysis demonstrated that polysaccharide synthesis at hyphal tips was dependent on CslA(Sc). cslA(Sc) was constitutively transcribed, and an enhanced green fluorescent protein-CslA(Sc) fusion protein was mostly located at the hyphal tips. An extract enriched in morphogenetic chaplin proteins promoted formation of aerial hyphae by the mutant. Furthermore, a two-hybrid experiment indicated that the glycosyltransferase domain of CslA(Sc) interacted with the tropomyosin-like polarity-determining DivIVA protein, suggesting that the tip-located DivIVA governed tip recruitment of the CslA(Sc) membrane protein. These results imply that the cellulose synthase-like protein couples extracellular and cytoskeletal components functioning in tip growth and cell development. PMID:18487344

  2. A Cellulose Synthase-Like Protein Involved in Hyphal Tip Growth and Morphological Differentiation in Streptomyces▿

    PubMed Central

    Xu, Hongbin; Chater, Keith F.; Deng, Zixin; Tao, Meifeng

    2008-01-01

    Cellulose synthase and cellulose synthase-like proteins, responsible for synthesizing β-glucan-containing polysaccharides, play a fundamental role in cellular architectures, such as plant cell and tissue morphogenesis, bacterial biofilm formation, and fruiting-body development. However, the roles of the proteins involved in the developmental process are not well understood. Here, we report that a cellulose synthase-like protein (CslASc) in Streptomyces has a function in hyphal tip growth and morphological differentiation. The cslASc replacement mutant showed pleiotropic defects, including the severe delay of aerial-hyphal formation and altered cell wall morphology. Calcofluor white fluorescence analysis demonstrated that polysaccharide synthesis at hyphal tips was dependent on CslASc. cslASc was constitutively transcribed, and an enhanced green fluorescent protein-CslASc fusion protein was mostly located at the hyphal tips. An extract enriched in morphogenetic chaplin proteins promoted formation of aerial hyphae by the mutant. Furthermore, a two-hybrid experiment indicated that the glycosyltransferase domain of CslASc interacted with the tropomyosin-like polarity-determining DivIVA protein, suggesting that the tip-located DivIVA governed tip recruitment of the CslASc membrane protein. These results imply that the cellulose synthase-like protein couples extracellular and cytoskeletal components functioning in tip growth and cell development. PMID:18487344

  3. Three prevacuolar compartment Rab GTPases impact Candida albicans hyphal growth.

    PubMed

    Johnston, Douglas A; Tapia, Arturo Luna; Eberle, Karen E; Palmer, Glen E

    2013-07-01

    Disruption of vacuolar biogenesis in the pathogenic yeast Candida albicans causes profound defects in polarized hyphal growth. However, the precise vacuolar pathways involved in yeast-hypha differentiation have not been determined. Previously we focused on Vps21p, a Rab GTPase involved in directing vacuolar trafficking through the late endosomal prevacuolar compartment (PVC). Herein, we identify two additional Vps21p-related GTPases, Ypt52p and Ypt53p, that colocalize with Vps21p and can suppress the hyphal defects of the vps21Δ/Δ mutant. Phenotypic analysis of gene deletion strains revealed that loss of both VPS21 and YPT52 causes synthetic defects in endocytic trafficking to the vacuole, as well as delivery of the virulence-associated vacuolar membrane protein Mlt1p from the Golgi compartment. Transcription of all three GTPase-encoding genes is increased under hyphal growth conditions, and overexpression of the transcription factor Ume6p is sufficient to increase the transcription of these genes. While only the vps21Δ/Δ single mutant has hyphal growth defects, these were greatly exacerbated in a vps21Δ/Δ ypt52Δ/Δ double mutant. On the basis of relative expression levels and phenotypic analysis of gene deletion strains, Vps21p is the most important of the three GTPases, followed by Ypt52p, while Ypt53p has an only marginal impact on C. albicans physiology. Finally, disruption of a nonendosomal AP-3-dependent vacuolar trafficking pathway in the vps21Δ/Δ ypt52Δ/Δ mutant, further exacerbated the stress and hyphal growth defects. These findings underscore the importance of membrane trafficking through the PVC in sustaining the invasive hyphal growth form of C. albicans.

  4. Colony patterning and collective hyphal growth of filamentous fungi

    NASA Astrophysics Data System (ADS)

    Matsuura, Shu

    2002-11-01

    Colony morphology of wild and mutant strains of Aspergillus nidulans at various nutrient and agar levels was investigated. Two types of colony patterning were found for these strains. One type produced uniform colonies at all nutrient and agar levels tested, and the other exhibited morphological change into disordered ramified colonies at low nutrient levels. Both types showed highly condensed compact colonies at high nutrient levels on low agar media that was highly diffusive. Disordered colonies were found to develop with low hyphal extension rates at low nutrient levels. To understand basic pattern selection rules, a colony model with three parameters, i.e., the initial nutrient level and the step length of nutrient random walk as the external parameters, and the frequency of nutrient uptake as an internal parameter, was constructed. At low nutrient levels, with decreasing nutrient uptake frequency under diffusive conditions, the model colony exhibited onsets of disordered ramification. Further, in the growth process of A. nidulans, reduction of hyphal extension rate due to a population effect of hyphae was found when hyphae form three-dimensional dense colonies, as compared to the case in which hyphal growth was restricted into two-dimensional space. A hyphal population effect was introduced in the colony model. Thickening of colony periphery due to the population effect became distinctive as the nutrient diffusion effect was raised at high nutrient levels with low hyphal growth rate. It was considered that colony patterning and onset of disorder were strongly governed by the combination of nutrient diffusion and hyphal growth rate.

  5. Bacterial peptidoglycan-derived molecules activate Candida albicans hyphal growth.

    PubMed

    Wang, Yue; Xu, Xiao-Li

    2008-01-01

    Serum strongly induces the yeast-to-hypha growth transition in the human fungal pathogen Candida albicans, playing an important role in infection. However, identity of the serum inducer(s) and its sensor remain poorly defined. We used NMR to analyze the chromatographic serum fractionations enriched for the hypha-inducing activity and found structures resembling subunits of bacterial peptidoglycan (PGN). We then confirmed that several purified and synthetic muramyl dipeptides (MDPs), subunits of PGN, can indeed strongly promote C. albicans hyphal growth. Taking cue from the recognition of MDPs by the mammalian bacterial sensor Nod2 using its leucine-rich-repeat (LRR) domain, we discovered that MDPs activate the adenylyl cyclase Cyr1 by binding to its LRR domain. The cAMP/PKA signaling pathway is well known to control hyphal morphogenesis and other infection-related traits. Given the abundance of PGN at the large intestinal epithelial surface, a natural habitat and invasion site for C. albcians, our findings have important implications in the mechanisms of infection by this pathogen. PMID:19704871

  6. Bacterial peptidoglycan-derived molecules activate Candida albicans hyphal growth

    PubMed Central

    Xu, Xiao-Li

    2008-01-01

    Serum strongly induces the yeast-to-hypha growth transition in the human fungal pathogen Candida albicans, playing an important role in infection. However, identity of the serum inducer(s) and its sensor remain poorly defined. We used NMR to analyze the chromatographic serum fractionations enriched for the hypha-inducing activity and found structures resembling subunits of bacterial peptidoglycan (PGN). We then confirmed that several purified and synthetic muramyl dipeptides (MDPs), subunits of PGN, can indeed strongly promote C. albicans hyphal growth. Taking cue from the recognition of MDPs by the mammalian bacterial sensor Nod2 using its leucine-rich-repeat (LRR) domain, we discovered that MDPs activate the adenylyl cyclase Cyr1 by binding to its LRR domain. The cAMP/PKA signaling pathway is well known to control hyphal morphogenesis and other infection-related traits. Given the abundance of PGN at the large intestinal epithelial surface, a natural habitat and invasion site for C. albcians, our findings have important implications in the mechanisms of infection by this pathogen. PMID:19704871

  7. Autophagy contributes to regulation of nuclear dynamics during vegetative growth and hyphal fusion in Fusarium oxysporum.

    PubMed

    Corral-Ramos, Cristina; Roca, M Gabriela; Di Pietro, Antonio; Roncero, M Isabel G; Ruiz-Roldán, Carmen

    2015-01-01

    In the fungal pathogen Fusarium oxysporum, vegetative hyphal fusion triggers nuclear mitotic division in the invading hypha followed by migration of a nucleus into the receptor hypha and degradation of the resident nucleus. Here we examined the role of autophagy in fusion-induced nuclear degradation. A search of the F. oxysporum genome database for autophagy pathway components identified putative orthologs of 16 core autophagy-related (ATG) genes in yeast, including the ubiquitin-like protein Atg8, which is required for the formation of autophagosomal membranes. F. oxysporum Foatg8Δ mutants were generated in a strain harboring H1-cherry fluorescent protein (ChFP)-labeled nuclei to facilitate analysis of nuclear dynamics. The Foatg8Δ mutants did not show MDC-positive staining in contrast to the wild type and the FoATG8-complemented (cFoATG8) strain, suggesting that FoAtg8 is required for autophagy in F. oxysporum. The Foatg8Δ strains displayed reduced rates of hyphal growth, conidiation, and fusion, and were significantly attenuated in virulence on tomato plants and in the nonvertebrate animal host Galleria mellonella. In contrast to wild-type hyphae, which are almost exclusively composed of uninucleated hyphal compartments, the hyphae of the Foatg8Δ mutants contained a significant fraction of hyphal compartments with 2 or more nuclei. The increase in the number of nuclei per hyphal compartment was particularly evident after hyphal fusion events. Time-lapse microscopy analyses revealed abnormal mitotic patterns during vegetative growth in the Foatg8Δ mutants. Our results suggest that autophagy mediates nuclear degradation after hyphal fusion and has a general function in the control of nuclear distribution in F. oxysporum.

  8. Autophagy contributes to regulation of nuclear dynamics during vegetative growth and hyphal fusion in Fusarium oxysporum.

    PubMed

    Corral-Ramos, Cristina; Roca, M Gabriela; Di Pietro, Antonio; Roncero, M Isabel G; Ruiz-Roldán, Carmen

    2015-01-01

    In the fungal pathogen Fusarium oxysporum, vegetative hyphal fusion triggers nuclear mitotic division in the invading hypha followed by migration of a nucleus into the receptor hypha and degradation of the resident nucleus. Here we examined the role of autophagy in fusion-induced nuclear degradation. A search of the F. oxysporum genome database for autophagy pathway components identified putative orthologs of 16 core autophagy-related (ATG) genes in yeast, including the ubiquitin-like protein Atg8, which is required for the formation of autophagosomal membranes. F. oxysporum Foatg8Δ mutants were generated in a strain harboring H1-cherry fluorescent protein (ChFP)-labeled nuclei to facilitate analysis of nuclear dynamics. The Foatg8Δ mutants did not show MDC-positive staining in contrast to the wild type and the FoATG8-complemented (cFoATG8) strain, suggesting that FoAtg8 is required for autophagy in F. oxysporum. The Foatg8Δ strains displayed reduced rates of hyphal growth, conidiation, and fusion, and were significantly attenuated in virulence on tomato plants and in the nonvertebrate animal host Galleria mellonella. In contrast to wild-type hyphae, which are almost exclusively composed of uninucleated hyphal compartments, the hyphae of the Foatg8Δ mutants contained a significant fraction of hyphal compartments with 2 or more nuclei. The increase in the number of nuclei per hyphal compartment was particularly evident after hyphal fusion events. Time-lapse microscopy analyses revealed abnormal mitotic patterns during vegetative growth in the Foatg8Δ mutants. Our results suggest that autophagy mediates nuclear degradation after hyphal fusion and has a general function in the control of nuclear distribution in F. oxysporum. PMID:25560310

  9. Autophagy contributes to regulation of nuclear dynamics during vegetative growth and hyphal fusion in Fusarium oxysporum

    PubMed Central

    Corral-Ramos, Cristina; Roca, M Gabriela; Di Pietro, Antonio; Roncero, M Isabel G; Ruiz-Roldán, Carmen

    2015-01-01

    In the fungal pathogen Fusarium oxysporum, vegetative hyphal fusion triggers nuclear mitotic division in the invading hypha followed by migration of a nucleus into the receptor hypha and degradation of the resident nucleus. Here we examined the role of autophagy in fusion-induced nuclear degradation. A search of the F. oxysporum genome database for autophagy pathway components identified putative orthologs of 16 core autophagy-related (ATG) genes in yeast, including the ubiquitin-like protein Atg8, which is required for the formation of autophagosomal membranes. F. oxysporum Foatg8Δ mutants were generated in a strain harboring H1-cherry fluorescent protein (ChFP)-labeled nuclei to facilitate analysis of nuclear dynamics. The Foatg8Δ mutants did not show MDC-positive staining in contrast to the wild type and the FoATG8-complemented (cFoATG8) strain, suggesting that FoAtg8 is required for autophagy in F. oxysporum. The Foatg8Δ strains displayed reduced rates of hyphal growth, conidiation, and fusion, and were significantly attenuated in virulence on tomato plants and in the nonvertebrate animal host Galleria mellonella. In contrast to wild-type hyphae, which are almost exclusively composed of uninucleated hyphal compartments, the hyphae of the Foatg8Δ mutants contained a significant fraction of hyphal compartments with 2 or more nuclei. The increase in the number of nuclei per hyphal compartment was particularly evident after hyphal fusion events. Time-lapse microscopy analyses revealed abnormal mitotic patterns during vegetative growth in the Foatg8Δ mutants. Our results suggest that autophagy mediates nuclear degradation after hyphal fusion and has a general function in the control of nuclear distribution in F. oxysporum. PMID:25560310

  10. Effects of Aeration of Sawdust Cultivation Bags on Hyphal Growth of Lentinula edodes.

    PubMed

    Lee, Hwa-Yong; Ham, Eun-Ju; Yoo, Young-Jin; Kim, Eui-Sung; Shim, Kyu-Kwang; Kim, Myung-Kon; Koo, Chang-Duck

    2012-09-01

    The effects of aeration through lid filters on the hyphal growth of Lentinula edodes (oak mushroom) in sawdust cultivation bags were investigated. The aeration treatment levels were traditional 27 mm hole cotton plugs, cotton balls and combinations of seven hole sizes × two hole positions (up and under) in the lids covering plastic bags containing 1.4 kg sawdust medium at 63% moisture that had been autoclaved for one hour and inoculated with sawdust spawn of L. edodes strain 921. Aeration treatment effects were measured based on the CO(2) concentration at the 15th wk, as well as the hyphal growth rate and degree of weight loss of bags every 14 days for 15 wk. In bags with traditional cotton plugs, the CO(2) concentration was 3.8 ± 1.3%, daily mean hyphal growth was 2.3 ± 0.6 mm and daily mean weight loss was 0.84 ± 0.26 g. In the bags with 15 mm diameter holes, the CO(2) concentration was 6.0 ± 1.6%, daily hyphal growth was 2.8 ± 0.2 mm and daily weight loss was 0.86 ± 0.4 g. The bags with 15 mm holes had a higher CO(2) concentration and lower water loss than bags with other hole sizes, but the hyphal growth was not significantly different from that of other bags. The weight loss of bags increased proportionally relative to the lid hole sizes. Taken together, these results indicate that traditional cotton plugs are economically efficient, but 15 mm hole lids are the most efficient at maintaining hyphal growth and controlling water loss while allowing CO(2) emissions.

  11. Boric acid destabilizes the hyphal cytoskeleton and inhibits invasive growth of Candida albicans.

    PubMed

    Pointer, Benjamin R; Boyer, Michael P; Schmidt, Martin

    2015-04-01

    Exposure of Candida albicans to sub-lethal concentrations of boric acid (BA) restricts the dimorphic fungus to its yeast morphology and prevents the formation of invasive hyphae on solid substrates. Exposure to BA causes a rapid and reversible disappearance of polarisome and Spitzenkörper in growing hyphae. In BA-treated hyphae of C. albicans, actin quickly reorganizes from cytoplasmic cables to cortical patches and cell wall growth switches from an apical to an isotropic pattern. As a result of the cytoskeletal changes, the hyphal tips broaden and directional growth of hyphae ceases in the presence of BA. An analysis of homozygous deletion strains showed that mutants with constitutive or enhanced hyphal growth (tup1, nrg1, ssn6, rbf1) are BA-sensitive, demonstrating that cellular morphology is a major determinant of BA tolerance. The screening of deletion mutants also showed that deficiencies of the main activator of hyphal gene expression, Efg1, and the Rim101-signalling cascade, leading to Efg1 activation, cause BA resistance. Taken together, the data presented show that the selective inhibitory effect on BA on C. albicans hyphae is rooted in a disruption of apical cytoskeletal elements of growing hyphae.

  12. Calcineurin Orchestrates Hyphal Growth, Septation, Drug Resistance and Pathogenesis of Aspergillus fumigatus: Where Do We Go from Here?

    PubMed Central

    Juvvadi, Praveen R; Steinbach, William J

    2015-01-01

    Studies on fungal pathogens belonging to the ascomycota phylum are critical given the ubiquity and frequency with which these fungi cause infections in humans. Among these species, Aspergillus fumigatus causes invasive aspergillosis, a leading cause of death in immunocompromised patients. Fundamental to A. fumigatus pathogenesis is hyphal growth. However, the precise mechanisms underlying hyphal growth and virulence are poorly understood. Over the past 10 years, our research towards the identification of molecular targets responsible for hyphal growth, drug resistance and virulence led to the elucidation of calcineurin as a key signaling molecule governing these processes. In this review, we summarize our salient findings on the significance of calcineurin for hyphal growth and septation in A. fumigatus and propose future perspectives on exploiting this pathway for designing new fungal-specific therapeutics. PMID:26694470

  13. Unisexual reproduction enhances fungal competitiveness by promoting habitat exploration via hyphal growth and sporulation.

    PubMed

    Phadke, Sujal S; Feretzaki, Marianna; Heitman, Joseph

    2013-08-01

    Unisexual reproduction is a novel homothallic sexual cycle recently discovered in both ascomycetous and basidiomycetous pathogenic fungi. It is a form of selfing that induces the yeast-to-hyphal dimorphic transition in isolates of the α mating type of the human fungal pathogen Cryptococcus neoformans. Unisexual reproduction may benefit the pathogen by facilitating sexual reproduction in the absence of the opposite a mating type and by generating infectious propagules called basidiospores. Here, we report an independent potential selective advantage of unisexual reproduction beyond genetic exchange and recombination. We competed a wild-type strain capable of undergoing unisexual reproduction with mutants defective in this developmental pathway and found that unisexual reproduction provides a considerable dispersal advantage through hyphal growth and sporulation. Our results show that unisexual reproduction may serve to facilitate access to both nutrients and potential mating partners and may provide a means to maintain the capacity for dimorphic transitions in the environment.

  14. Differential chlorate inhibition of Chaetomium globosum germination, hyphal growth, and perithecia synthesis.

    PubMed

    Biles, Charles L; Wright, Desiree; Fuego, Marianni; Guinn, Angela; Cluck, Terry; Young, Jennifer; Martin, Markie; Biles, Josiah; Poudyal, Shubhra

    2012-12-01

    Chaetomium globosum Kunze:Fr is a dermatophytic, dematiaceous fungus that is ubiquitous in soils, grows readily on cellulolytic materials, and is commonly found on water-damaged building materials. Chlorate affects nitrogen metabolism in fungi and is used to study compatibility among anamorphic fungi by inducing nit mutants. The effect of chlorate toxicity on C. globosum was investigated by amending a modified malt extract agar (MEA), oat agar, and carboxymethyl cellulose agar (CMC) with various levels of potassium chlorate (KClO(3)). C. globosum perithecia production was almost completely inhibited (90-100 %) at low levels of KClO(3) (0.1 mM) in amended MEA. Inhibition of perithecia production was also observed on oat agar and CMC at 1 and 10 mM, respectively. However, hyphal growth in MEA was only inhibited 20 % by 0.1-100 mM KClO(3) concentrations. Hyphal growth was never completely inhibited at the highest levels tested (200 mM). Higher levels of KClO(3) were needed on gypsum board to inhibit perithecia synthesis. In additional experiments, KClO(3) did not inhibit C. globosum, Fusarium oxysporum, Aspergillus niger, Penicillum expansum, and airborne fungal spore germination. The various fungal spores were not inhibited by KClO(3) at 1-100 mM levels. These results suggest that C. globosum perithecia synthesis is more sensitive to chlorate toxicity than are hyphal growth and spore germination. This research provides basic information that furthers our understanding about perithecia formation and may help in developing control methods for fungal growth on building materials.

  15. Identification of a gene involved in the regulation of hyphal growth of Epichloë festucae during symbiosis.

    PubMed

    Bassett, Shalome A; Johnson, Richard D; Simpson, Wayne R; Laugraud, Aurelie; Jordan, T William; Bryan, Gregory T

    2016-10-01

    Secreted proteins, those involved in cell wall biogenesis, are likely to play a role in communication in the symbiotic interaction between the fungal endophyte Epichloë festucae with perennial ryegrass (Lolium perenne), particularly given the close association between fungal hyphae and the plant cell wall. Our hypothesis was that secreted proteins are likely to be responsible for establishing and maintaining a normal symbiotic relationship. We analyzed an endophyte EST database for genes with predicted signal peptide sequences. Here, we report the identification and characterization of rhgA; a gene involved in the regulation of hyphal growth in planta In planta analysis of ΔrhgA mutants showed that disruption of rhgA resulted in extensive unregulated hyphal growth. This phenotype was fully complemented by insertion of the rhgA gene and suggests that rhgA is important for maintaining normal hyphal growth during symbiosis. PMID:27624305

  16. Hbr1 Activates and Represses Hyphal Growth in Candida albicans and Regulates Fungal Morphogenesis under Embedded Conditions

    PubMed Central

    Pendrak, Michael L.; Roberts, David D.

    2015-01-01

    Transitions between yeast and hyphae are essential for Candida albicans pathogenesis. The genetic programs that regulate its hyphal development can be distinguished by embedded versus aerobic surface agar invasion. Hbr1, a regulator of white-opaque switching, is also a positive and negative regulator of hyphal invasion. During embedded growth at 24°C, an HBR1/hbr1 strain formed constitutively filamentous colonies throughout the matrix, resembling EFG1 null colonies, and a subset of long unbranched hyphal aggregates enclosed in a spindle-shaped capsule. Inhibition of adenylate cyclase with farnesol perturbed the filamentation of HBR1/hbr1 cells producing cytokinesis-defective hyphae whereas farnesol treated EFG1 null cells produced abundant opaque-like cells. Point mutations in the Hbr1 ATP-binding domain caused distinct filamentation phenotypes including uniform radial hyphae, hyphal sprouts, and massive yeast cell production. Conversely, aerobic surface colonies of the HBR1 heterozygote on Spider and GlcNAc media lacked filamentation that could be rescued by growth under low (5%) O2. Consistent with these morphogenesis defects, the HBR1 heterozygote exhibited attenuated virulence in a mouse candidemia model. These data define Hbr1 as an ATP-dependent positive and negative regulator of hyphal development that is sensitive to hypoxia. PMID:26039220

  17. FvSO regulates vegetative hyphal fusion, asexual growth, fumonisin B1 production, and virulence in Fusarium verticillioides.

    PubMed

    Guo, Li; Wenner, Nancy; Kuldau, Gretchen A

    2015-12-01

    Hyphal anastomosis is a hallmark of filamentous fungi and plays vital roles including cellular homoeostasis, interhyphal communication and nutrient translocation. Here we identify a gene, FvSO, in Fusarium verticillioides, a filamentous ascomycete causing maize ear and stalk rot and producing fumonisin mycotoxins. FvSO, like its Neurospora crassa homologue SO, is required for vegetative hyphal fusion. It is also essential for normal vegetative growth, sporulation, and pathogenesis. FvSO encodes a predicted WW domain protein and shares 70 % protein sequence identity with N. crassa SO. FvSO deletion mutants (ΔFvSO) had abnormal distribution of conidia size, and conidia of ΔFvSO germinated much later and slower than wild type. ΔFvSO was deficient in hyphal anastomosis, had slower radial growth and produced less fungal biomass than wild type. ΔFvSO were unable to perform anastomosis, a key feature of filamentous fungi. Interestingly, production of fumonisin B1 by ΔFvSO was significantly reduced compared to wild type. Additionally, ΔFvSO was nonpathogenic to corn ears, stalks and seedlings, likely due to defective growth and development. In conclusion, FvSO is essential for vegetative hyphal fusion and is required for normal vegetative growth and sporulation, normal levels of fumonisin production and pathogenicity in F. verticillioides. The pleiotropic nature of ΔFvSO phenotypes suggests that FvSO is likely involved in certain signalling pathways that regulate multiple cellular functions.

  18. The ham-2 locus, encoding a putative transmembrane protein, is required for hyphal fusion in Neurospora crassa.

    PubMed Central

    Xiang, Qijun; Rasmussen, Carolyn; Glass, N Louise

    2002-01-01

    Somatic cell fusion is common during organogenesis in multicellular eukaryotes, although the molecular mechanism of cell fusion is poorly understood. In filamentous fungi, somatic cell fusion occurs during vegetative growth. Filamentous fungi grow as multinucleate hyphal tubes that undergo frequent hyphal fusion (anastomosis) during colony expansion, resulting in the formation of a hyphal network. The molecular mechanism of the hyphal fusion process and the role of networked hyphae in the growth and development of these organisms are unexplored questions. We use the filamentous fungus Neurospora crassa as a model to study the molecular mechanism of hyphal fusion. In this study, we identified a deletion mutant that was restricted in its ability to undergo both self-hyphal fusion and fusion with a different individual to form a heterokaryon. This deletion mutant displayed pleiotropic defects, including shortened aerial hyphae, altered conidiation pattern, female sterility, slow growth rate, lack of hyphal fusion, and suppression of vegetative incompatibility. Complementation with a single open reading frame (ORF) within the deletion region in this mutant restored near wild-type growth rates, female fertility, aerial hyphae formation, and hyphal fusion, but not vegetative incompatibility and wild-type conidiation pattern. This ORF, which we named ham-2 (for hyphal anastomosis), encodes a putative transmembrane protein that is highly conserved, but of unknown function among eukaryotes. PMID:11805054

  19. The Spatial Distribution of the Exocyst and Actin Cortical Patches Is Sufficient To Organize Hyphal Tip Growth

    PubMed Central

    Caballero-Lima, David; Kaneva, Iliyana N.; Watton, Simon P.

    2013-01-01

    In the hyphal tip of Candida albicans we have made detailed quantitative measurements of (i) exocyst components, (ii) Rho1, the regulatory subunit of (1,3)-β-glucan synthase, (iii) Rom2, the specialized guanine-nucleotide exchange factor (GEF) of Rho1, and (iv) actin cortical patches, the sites of endocytosis. We use the resulting data to construct and test a quantitative 3-dimensional model of fungal hyphal growth based on the proposition that vesicles fuse with the hyphal tip at a rate determined by the local density of exocyst components. Enzymes such as (1,3)-β-glucan synthase thus embedded in the plasma membrane continue to synthesize the cell wall until they are removed by endocytosis. The model successfully predicts the shape and dimensions of the hyphae, provided that endocytosis acts to remove cell wall-synthesizing enzymes at the subapical bands of actin patches. Moreover, a key prediction of the model is that the distribution of the synthase is substantially broader than the area occupied by the exocyst. This prediction is borne out by our quantitative measurements. Thus, although the model highlights detailed issues that require further investigation, in general terms the pattern of tip growth of fungal hyphae can be satisfactorily explained by a simple but quantitative model rooted within the known molecular processes of polarized growth. Moreover, the methodology can be readily adapted to model other forms of polarized growth, such as that which occurs in plant pollen tubes. PMID:23666623

  20. The FUS3/KSS1-type MAP kinase gene FPK1 is involved in hyphal growth, conidiation and plant infection of Fusarium proliferatum.

    PubMed

    Zhao, Pei-Bao; Ren, Ai-Zhi; Li, Duo-Chuan

    2011-01-01

    Fusarium proliferatum is an important pathogen of maize that is responsible for ear rots, stalk rots and seeding blight worldwide. During the past decade, F. proliferatum has caused several severe epidemics of maize seedling blight in many areas of China, which led to significant losses in maize. To understand the molecular mechanisms in the fungal developmental regulation and pathogenicity, we isolated and characterized the FPK1 gene (GenBank accession No. HQ844224) encoding a MAP kinase homolog of FUS3/KSS1 in yeast. The gene includes a 1,242-bp DNA sequence from ATG to TAA, with a coding region of 1,068 bp, 3 introns (58 bp, 56 bp and 60 bp) and a predicted protein of 355 aa.The mutant ΔFPK1, which has a disruption of the FPK1 gene, showed reduced vegetative growth, fewer and shorter aerial mycelia, strongly impaired conidiation and spore germination, as well as deviant germ tube outgrowth. When the strain was inoculated in susceptible maize varieties, the infection of the mutant ΔFPK1 was delayed, and the infection efficiency was reduced compared to the wild-type strain. Complementation of the disruptions within the FPK1 open reading frame restored wild-type levels of conidiation, growth rate and virulence to maize seedlings. Our results indicated that the FPK1 gene functioned in hyphal growth, conidiation, spore germination and virulence in F. proliferatum. PMID:22286038

  1. Influence of soil organic matter decomposition on arbuscular mycorrhizal fungi in terms of asymbiotic hyphal growth and root colonization.

    PubMed

    Gryndler, Milan; Hrselová, Hana; Cajthaml, Tomás; Havránková, Marie; Rezácová, Veronika; Gryndlerová, Hana; Larsen, John

    2009-04-01

    Soil organic matter is known to influence arbuscular mycorrhizal (AM) fungi, but limited information is available on the chemical components in the organic matter causing these effects. We studied the influence of decomposing organic matter (pure cellulose and alfalfa shoot and root material) on AM fungi after 30, 100, and 300 days of decomposition in nonsterile soil with and without addition of mineral N and P. Decomposing organic matter affected maize root length colonized by the AM fungus Glomus claroideum in a similar manner as other plant growth parameters. Colonized root length was slightly increased by both nitrogen and phosphorus application and plant materials, but not by application of cellulose. In vitro hyphal growth of Glomus intraradices was increased by soil extracts from the treatments with all types of organic materials independently of mineral N and P application. Pyrolysis of soil samples from the different decomposition treatments revealed in total 266 recognizable organic compounds and in vitro hyphal growth of G. intraradices in soil extract positively correlated with 33 of these compounds. The strongest correlation was found with 3,4,5-trimethoxybenzoic acid methyl ester. This compound is a typical product of pyrolysis of phenolic compounds produced by angiosperm woody plants, but in our experiment, it was produced mainly from cellulose by some components of the soil microflora. In conclusion, our results indicate that mycelia of AM fungi are influenced by organic matter decomposition both via compounds released during the decomposition process and also by secondary metabolites produced by microorganisms involved in organic matter decomposition.

  2. The Gβ-like protein CpcB is required for hyphal growth, conidiophore morphology and pathogenicity in Aspergillus fumigatus.

    PubMed

    Cai, Zhen-dong; Chai, Yan-fei; Zhang, Cai-yun; Qiao, Wei-ran; Sang, Hong; Lu, Ling

    2015-08-01

    CpcB (cross pathway control B) encodes a yeast Cpc2 and mammalian RACK1 (receptor for activated protein kinase C) ortholog, which is a WD repeat protein with functional homology to the β subunit of heterotrimeric G proteins in Aspergillus fumigatus. Previous study has reported that CpcB governs growth and development in both A. fumigatus and Aspergillus nidulans. However, little is known about the functional identities of CpcB orthologs and their relationships with G protein complexes. In this study, we verified that cytoplasmic AfCpcB acts as a Gβ-like protein ortholog and plays important roles in hyphal growth, conidiophore morphology, cell wall integrity, and virulence in A. fumigatus. Furthermore, double deletion of AfcpcB and AfgpaB (Gα) causes a similar phenotype to AfgpaB mutant with abnormal multiple septa conidiophores but exhibits sparse conidiation with white and fluffy colonies. Thus, the exacerbated conidiation defect suggests that AfcpcB has its own specific function compared to the Gα subunit of AfgpaB or the G-protein complex. In addition, complementation assays using AfcpcB orthologs of A. nidulans and yeasts (Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida albicans) suggest that all tested fungal AfcpcB orthologs under the A. fumigatus native promoter can largely restore hyphal growth defects in AfcpcB deletion mutant, but only the A. nidulans cpcB ortholog completely rescues the ΔAfcpcB conidiation defect, suggesting that CpcB acts as a Gβ-like protein ortholog in the Aspergilli, but may have unique and important unexplored functions that required for conidiation, which is absent in yeast. PMID:25892048

  3. Candida-streptococcal mucosal biofilms display distinct structural and virulence characteristics depending on growth conditions and hyphal morphotypes.

    PubMed

    Bertolini, M M; Xu, H; Sobue, T; Nobile, C J; Del Bel Cury, A A; Dongari-Bagtzoglou, A

    2015-08-01

    Candida albicans and streptococci of the mitis group form communities in multiple oral sites, where moisture and nutrient availability can change spatially or temporally. This study evaluated structural and virulence characteristics of Candida-streptococcal biofilms formed on moist or semidry mucosal surfaces, and tested the effects of nutrient availability and hyphal morphotype on dual-species biofilms. Three-dimensional models of the oral mucosa formed by immortalized keratinocytes on a fibroblast-embedded collagenous matrix were used. Infections were carried out using Streptococcus oralis strain 34, in combination with a C. albicans wild-type strain, or pseudohyphal-forming mutant strains. Increased moisture promoted a homogeneous surface biofilm by C. albicans. Dual biofilms had a stratified structure, with streptococci growing in close contact with the mucosa and fungi growing on the bacterial surface. Under semidry conditions, Candida formed localized foci of dense growth, which promoted focal growth of streptococci in mixed biofilms. Candida biofilm biovolume was greater under moist conditions, albeit with minimal tissue invasion, compared with semidry conditions. Supplementing the infection medium with nutrients under semidry conditions intensified growth, biofilm biovolume and tissue invasion/damage, without changing biofilm structure. Under these conditions, the pseudohyphal mutants and S. oralis formed defective superficial biofilms, with most bacteria in contact with the epithelial surface, below a pseudohyphal mass, resembling biofilms growing in a moist environment. The presence of S. oralis promoted fungal invasion and tissue damage under all conditions. We conclude that moisture, nutrient availability, hyphal morphotype and the presence of commensal bacteria influence the architecture and virulence characteristics of mucosal fungal biofilms. PMID:25754666

  4. Candida-streptococcal mucosal biofilms display distinct structural and virulence characteristics depending on growth conditions and hyphal morphotypes.

    PubMed

    Bertolini, M M; Xu, H; Sobue, T; Nobile, C J; Del Bel Cury, A A; Dongari-Bagtzoglou, A

    2015-08-01

    Candida albicans and streptococci of the mitis group form communities in multiple oral sites, where moisture and nutrient availability can change spatially or temporally. This study evaluated structural and virulence characteristics of Candida-streptococcal biofilms formed on moist or semidry mucosal surfaces, and tested the effects of nutrient availability and hyphal morphotype on dual-species biofilms. Three-dimensional models of the oral mucosa formed by immortalized keratinocytes on a fibroblast-embedded collagenous matrix were used. Infections were carried out using Streptococcus oralis strain 34, in combination with a C. albicans wild-type strain, or pseudohyphal-forming mutant strains. Increased moisture promoted a homogeneous surface biofilm by C. albicans. Dual biofilms had a stratified structure, with streptococci growing in close contact with the mucosa and fungi growing on the bacterial surface. Under semidry conditions, Candida formed localized foci of dense growth, which promoted focal growth of streptococci in mixed biofilms. Candida biofilm biovolume was greater under moist conditions, albeit with minimal tissue invasion, compared with semidry conditions. Supplementing the infection medium with nutrients under semidry conditions intensified growth, biofilm biovolume and tissue invasion/damage, without changing biofilm structure. Under these conditions, the pseudohyphal mutants and S. oralis formed defective superficial biofilms, with most bacteria in contact with the epithelial surface, below a pseudohyphal mass, resembling biofilms growing in a moist environment. The presence of S. oralis promoted fungal invasion and tissue damage under all conditions. We conclude that moisture, nutrient availability, hyphal morphotype and the presence of commensal bacteria influence the architecture and virulence characteristics of mucosal fungal biofilms.

  5. Libraries for two-hybrid screening of yeast and hyphal growth forms in Zymoseptoria tritici.

    PubMed

    Ma, W; Kilaru, S; Collins, C; Courbot, M; Steinberg, G

    2015-06-01

    Pathogenic fungi are constantly emerging resistance to anti-fungal treatments. Therefore, identification of new fungicide targets is important. Good candidates are essential fungal proteins and their regulators. An efficient way to reveal the molecular environment of an essential protein is the search for interacting factors. Here, we establish three yeast two-hybrid libraries, covering yeast and hyphal stages of the wheat pathogen Zymoseptoria tritici. No detectable genomic DNA was present in any of the 3 libraries. Random amplification revealed that the libraries include cDNA fragments of up to 2000bp, suggesting that small-to-medium sized proteins are represented therein. Indeed, full-length cDNAs of five proteins were found in all libraries. The full-length cDNA of large chitin synthase gene mcs1 (5742bp with introns; 5568bp without introns) could not be amplified, but its 5' and 3' regions were represented, suggesting that even larger genes are covered in all libraries. Finally, we tested for the expected interaction of the autophagy proteins ZtAtg4 and ZtAtg8 in Z. tritici, and then used ZtAtg4 to screen one of the two-hybrid libraries. Indeed, we found ZtAtg8 as a positive interaction partner, confirming that interacting proteins can be identified. Thus, these molecular tools promise to be useful in identifying novel fungicide target proteins.

  6. The expression of the cerato-platanin gene is related to hyphal growth and chlamydospores formation in Ceratocystis platani.

    PubMed

    Baccelli, Ivan; Comparini, Cecilia; Bettini, Priscilla P; Martellini, Federica; Ruocco, Michelina; Pazzagli, Luigia; Bernardi, Rodolfo; Scala, Aniello

    2012-02-01

    Cerato-platanin (CP) is a protein produced by Ceratocystis platani, the causal agent of canker stain disease of plane trees. CP is the first member of the 'cerato-platanin family', and its role as a pathogen-associated molecular pattern (PAMP), inducing defence responses both in host and nonhost plants, is established. However, the primary role of CP and its homologues in the fungal life remains unknown. In the present work, we investigated the regulation of the cp gene during the in vitro growth of C. platani in different conditions and under the effect of potential stress factors. Fungal growth and conidiogenesis were also analysed. Results showed that cp is a single-copy gene whose expression level is strictly associated with hyphal growth and with chlamydospores formation. The analysis of a 1368 bp 5'-flanking region revealed putative motifs that could be involved in the regulation of gene expression in response to stress and developmental cues. Taking into account the localization of CP in the fungal cell wall and the recently published 3D structure of the protein, our results support a role for CP in growth and developmental processes of C. platani.

  7. UvHOG1 is important for hyphal growth and stress responses in the rice false smut fungus Ustilaginoidea virens.

    PubMed

    Zheng, Dawei; Wang, Yi; Han, Yu; Xu, Jin-Rong; Wang, Chenfang

    2016-01-01

    Rice false smut caused by Ustilaginoidea virens is one of the most important diseases of rice worldwide. Although its genome has been sequenced, to date there is no report on targeted gene deletion in U. virens and no molecular studies on genetic mechanisms regulating the infection processes of this destructive pathogen. In this study, we attempted to generate knockout mutants of the ortholog of yeast HOG1 MAP kinase gene in U. virens. One Uvhog1 deletion mutant was identified after screening over 600 hygromycin-resistant transformants generated by Agrobacterium tumefaciens mediated transformation. The Uvhog1 mutant was reduced in growth rate and conidiation but had increased sensitivities to SDS, Congo red, and hyperosmotic stress. Deletion of UvHOG1 resulted in reduced expression of the stress response-related genes UvATF1 and UvSKN7. In the Uvhog1 mutant, NaCl treatment failed to stimulate the accumulation of sorbitol and glycerol. In addition, the Uvhog1 mutant had reduced toxicity on shoot growth in rice seed germination assays. Overall, as the first report of targeted gene deletion mutant in U. virens, our results showed that UvHOG1 likely has conserved roles in regulating stress responses, hyphal growth, and possibly secondary metabolism. PMID:27095476

  8. UvHOG1 is important for hyphal growth and stress responses in the rice false smut fungus Ustilaginoidea virens

    PubMed Central

    Zheng, Dawei; Wang, Yi; Han, Yu; Xu, Jin-Rong; Wang, Chenfang

    2016-01-01

    Rice false smut caused by Ustilaginoidea virens is one of the most important diseases of rice worldwide. Although its genome has been sequenced, to date there is no report on targeted gene deletion in U. virens and no molecular studies on genetic mechanisms regulating the infection processes of this destructive pathogen. In this study, we attempted to generate knockout mutants of the ortholog of yeast HOG1 MAP kinase gene in U. virens. One Uvhog1 deletion mutant was identified after screening over 600 hygromycin-resistant transformants generated by Agrobacterium tumefaciens mediated transformation. The Uvhog1 mutant was reduced in growth rate and conidiation but had increased sensitivities to SDS, Congo red, and hyperosmotic stress. Deletion of UvHOG1 resulted in reduced expression of the stress response-related genes UvATF1 and UvSKN7. In the Uvhog1 mutant, NaCl treatment failed to stimulate the accumulation of sorbitol and glycerol. In addition, the Uvhog1 mutant had reduced toxicity on shoot growth in rice seed germination assays. Overall, as the first report of targeted gene deletion mutant in U. virens, our results showed that UvHOG1 likely has conserved roles in regulating stress responses, hyphal growth, and possibly secondary metabolism. PMID:27095476

  9. Hyphal chemotropism in fungal pathogenicity.

    PubMed

    Turrà, David; Nordzieke, Daniela; Vitale, Stefania; El Ghalid, Mennat; Di Pietro, Antonio

    2016-09-01

    The ability to grow as filamentous hyphae defines the lifestyle of fungi. Hyphae are exposed to a variety of chemical stimuli such as nutrients or signal molecules from mating partners and host organisms. How fungi sense and process this chemical information to steer hyphal growth is poorly understood. Saccharomyces cerevisiae and Neurospora crassa have served as genetic models for the identification of cellular components functioning in chemotropism. A recent study in the pathogen Fusarium oxysporum revealed distinct MAPK pathways governing hyphal growth towards nutrient sources and sex pheromones or plant signals, suggesting an unanticipated complexity of chemosensing during fungus-host interactions. PMID:27150623

  10. A cytoplasmic Cu-Zn superoxide dismutase SOD1 contributes to hyphal growth and virulence of Fusarium graminearum.

    PubMed

    Yao, Sheng-Hua; Guo, Yan; Wang, Yan-Zhang; Zhang, Dong; Xu, Ling; Tang, Wei-Hua

    2016-06-01

    Superoxide dismutases (SODs) are scavengers of superoxide radicals, one of the main reactive oxygen species (ROS) in the cell. SOD-based ROS scavenging system constitutes the frontline defense against intra- and extracellular ROS, but the roles of SODs in the important cereal pathogen Fusarium graminearum are not very clear. There are five SOD genes in F. graminearum genome, encoding cytoplasmic Cu-Zn SOD1 and MnSOD3, mitochondrial MnSOD2 and FeSOD4, and extracellular CuSOD5. Previous studies reported that the expression of SOD1 increased during infection of wheat coleoptiles and florets. In this work we showed that the recombinant SOD1 protein had the superoxide dismutase activity in vitro, and that the SOD1-mRFP fusion protein localized in the cytoplasm of F. graminearum. The Δsod1 mutants had slightly reduced hyphal growth and markedly increased sensitivity to the intracellular ROS generator menadione. The conidial germination under extracellular oxidative stress was significantly delayed in the mutants. Wheat floret infection assay showed that the Δsod1 mutants had a reduced pathogenicity. Furthermore, the Δsod1 mutants had a significant reduction in production of deoxynivalenol mycotoxin. Our results indicate that the cytoplasmic Cu-Zn SOD1 affects fungal growth probably depending on detoxification of intracellular superoxide radicals, and that SOD1-mediated deoxynivalenol production contributes to the virulence of F. graminearum in wheat head infection. PMID:27037138

  11. Turgor regulation in hyphal organisms.

    PubMed

    Lew, Roger R; Levina, Natalia N; Walker, Sophie K; Garrill, Ashley

    2004-11-01

    Turgor regulation in two saprophytic hyphal organisms was examined directly with the pressure probe technique. The ascomycete Neurospora crassa, a terrestrial fungi, regulates turgor after hyperosmotic treatments when growing in a minimal medium containing K(+), Mg(2+), Ca(2+), Cl(-), and sucrose. Turgor recovery by N. crassa after hyperosmotic treatment is concurrent with changes in ion transport: hyperpolarization of the plasma membrane potential and a decline in transmembrane ion conductance. In contrast the oomycete Achlya bisexualis, a freshwater hyphal organism, does not regulate turgor after hyperosmotic treatment, although small transient increases in turgor were occasionally observed. We also monitored turgor in both organisms during hypoosmotic treatment and did not observe a turgor increase, possibly due to turgor regulation. Both hyphal organisms grow with similar morphologies, cellular expansion rates and turgor (0.4-0.7 MPa), yet respond differently to osmotic stress. The results do not support the assumption of a universal mechanism of tip growth driven by cell turgor.

  12. Influence of calcium on fungal growth, hyphal morphology and citric acid production in Aspergillus niger.

    PubMed

    Pera, L M; Callieri, D A

    1997-01-01

    Addition of 0.5 g/L CaCl2 to the fermentation medium lowered the final biomass dry mass by 35% and increased the uptake of phosphate and sucrose, and the production of citric acid by 15, 35 and 50%, respectively. In a medium deprived of Ca2+ the microorganism displayed both a pelleted and a filamentous form of growth, the hyphae being scarcely branched, without bulbous cells. An addition of Ca2+ induced a pelleted form of growth, highly branched hyphae and numerous bulbous cells. Bulbous cells growing in the presence of Ca2+ exhibited cell walls composed of laminated layers, and featured vesicles associated with the wall and/or the cell membrane, containing numerous inclusions. The cytotoxic effect of high concentrations of citric acid in the medium as well as an increase of the activity of N-acetyl-beta-D-glucosaminidase, a lytic enzyme, might be involved in these morphological changes.

  13. Candida albicans AGE3, the Ortholog of the S. cerevisiae ARF-GAP-Encoding Gene GCS1, Is Required for Hyphal Growth and Drug Resistance

    PubMed Central

    Lettner, Thomas; Zeidler, Ute; Gimona, Mario; Hauser, Michael; Breitenbach, Michael; Bito, Arnold

    2010-01-01

    Background Hyphal growth and multidrug resistance of C. albicans are important features for virulence and antifungal therapy of this pathogenic fungus. Methodology/Principal Findings Here we show by phenotypic complementation analysis that the C. albicans gene AGE3 is the functional ortholog of the yeast ARF-GAP-encoding gene GCS1. The finding that the gene is required for efficient endocytosis points to an important functional role of Age3p in endosomal compartments. Most C. albicans age3Δ mutant cells which grew as cell clusters under yeast growth conditions showed defects in filamentation under different hyphal growth conditions and were almost completely disabled for invasive filamentous growth. Under hyphal growth conditions only a fraction of age3Δ cells shows a wild-type-like polarization pattern of the actin cytoskeleton and lipid rafts. Moreover, age3Δ cells were highly susceptible to several unrelated toxic compounds including antifungal azole drugs. Irrespective of the AGE3 genotype, C-terminal fusions of GFP to the drug efflux pumps Cdr1p and Mdr1p were predominantly localized in the plasma membrane. Moreover, the plasma membranes of wild-type and age3Δ mutant cells contained similar amounts of Cdr1p, Cdr2p and Mdr1p. Conclusions/Significance The results indicate that the defect in sustaining filament elongation is probably caused by the failure of age3Δ cells to polarize the actin cytoskeleton and possibly of inefficient endocytosis. The high susceptibility of age3Δ cells to azoles is not caused by inefficient transport of efflux pumps to the cell membrane. A possible role of a vacuolar defect of age3Δ cells in drug susceptibility is proposed and discussed. In conclusion, our study shows that the ARF-GAP Age3p is required for hyphal growth which is an important virulence factor of C. albicans and essential for detoxification of azole drugs which are routinely used for antifungal therapy. Thus, it represents a promising antifungal drug target

  14. Zinc and Manganese Chelation by Neutrophil S100A8/A9 (Calprotectin) Limits Extracellular Aspergillus fumigatus Hyphal Growth and Corneal Infection.

    PubMed

    Clark, Heather L; Jhingran, Anupam; Sun, Yan; Vareechon, Chairut; de Jesus Carrion, Steven; Skaar, Eric P; Chazin, Walter J; Calera, José Antonio; Hohl, Tobias M; Pearlman, Eric

    2016-01-01

    Calprotectin, a heterodimer of S100A8 and S100A9, is an abundant neutrophil protein that possesses antimicrobial activity primarily because of its ability to chelate zinc and manganese. In the current study, we showed that neutrophils from calprotectin-deficient S100A9(-/-) mice have an impaired ability to inhibit Aspergillus fumigatus hyphal growth in vitro and in infected corneas in a murine model of fungal keratitis; however, the ability to inhibit hyphal growth was restored in S100A9(-/-) mice by injecting recombinant calprotectin. Furthermore, using recombinant calprotectin with mutations in either the Zn and Mn binding sites or the Mn binding site alone, we show that both zinc and manganese binding are necessary for calprotectin's antihyphal activity. In contrast to hyphae, we found no role for neutrophil calprotectin in uptake or killing of intracellular A. fumigatus conidia either in vitro or in a murine model of pulmonary aspergillosis. We also found that an A. fumigatus ∆zafA mutant, which demonstrates deficient zinc transport, exhibits impaired growth in infected corneas and following incubation with neutrophils or calprotectin in vitro as compared with wild-type. Collectively, these studies demonstrate a novel stage-specific susceptibility of A. fumigatus to zinc and manganese chelation by neutrophil-derived calprotectin.

  15. The F-actin capping protein is required for hyphal growth and full virulence but is dispensable for septum formation in Botrytis cinerea.

    PubMed

    González-Rodríguez, Victoria E; Garrido, Carlos; Cantoral, Jesús M; Schumacher, Julia

    2016-10-01

    Filamentous (F-) actin is an integral part of the cytoskeleton allowing for cell growth, intracellular motility, and cytokinesis of eukaryotic cells. Its assembly from G-actin monomers and its disassembly are tightly regulated processes involving a number of actin-binding proteins (ABPs) such as F-actin nucleators and cross-linking proteins. F-actin capping protein (CP) is an alpha/beta heterodimer known from yeast and higher eukaryotes to bind to the fast growing ends of the actin filaments stabilizing them. In this study, we identified the orthologs of the two CP subunits, named BcCPA1 and BcCPB1, in the plant pathogenic fungus Botrytis cinerea and showed that the two proteins physically interact in a yeast two-hybrid approach. GFP-BcCPA1 fusion proteins were functional and localized to the assumed sites of F-actin accumulation, i.e. to the hyphal tips and the sites of actin ring formation. Deletion of bccpa1 had a profound effect on hyphal growth, morphogenesis, and virulence indicating the importance of F-actin capping for an intact actin cytoskeleton. As polarized growth - unlike septum formation - is impaired in the mutants, it can be concluded that the organization and/or localization of actin patches and cables are disturbed rather than the functionality of the actin rings.

  16. The F-actin capping protein is required for hyphal growth and full virulence but is dispensable for septum formation in Botrytis cinerea.

    PubMed

    González-Rodríguez, Victoria E; Garrido, Carlos; Cantoral, Jesús M; Schumacher, Julia

    2016-10-01

    Filamentous (F-) actin is an integral part of the cytoskeleton allowing for cell growth, intracellular motility, and cytokinesis of eukaryotic cells. Its assembly from G-actin monomers and its disassembly are tightly regulated processes involving a number of actin-binding proteins (ABPs) such as F-actin nucleators and cross-linking proteins. F-actin capping protein (CP) is an alpha/beta heterodimer known from yeast and higher eukaryotes to bind to the fast growing ends of the actin filaments stabilizing them. In this study, we identified the orthologs of the two CP subunits, named BcCPA1 and BcCPB1, in the plant pathogenic fungus Botrytis cinerea and showed that the two proteins physically interact in a yeast two-hybrid approach. GFP-BcCPA1 fusion proteins were functional and localized to the assumed sites of F-actin accumulation, i.e. to the hyphal tips and the sites of actin ring formation. Deletion of bccpa1 had a profound effect on hyphal growth, morphogenesis, and virulence indicating the importance of F-actin capping for an intact actin cytoskeleton. As polarized growth - unlike septum formation - is impaired in the mutants, it can be concluded that the organization and/or localization of actin patches and cables are disturbed rather than the functionality of the actin rings. PMID:27647239

  17. The SH3/PH domain protein AgBoi1/2 collaborates with the Rho-type GTPase AgRho3 to prevent nonpolar growth at hyphal tips of Ashbya gossypii.

    PubMed

    Knechtle, Philipp; Wendland, Jürgen; Philippsen, Peter

    2006-10-01

    Unlike most other cells, hyphae of filamentous fungi permanently elongate and lack nonpolar growth phases. We identified AgBoi1/2p in the filamentous ascomycete Ashbya gossypii as a component required to prevent nonpolar growth at hyphal tips. Strains lacking AgBoi1/2p frequently show spherical enlargement at hyphal tips with concomitant depolarization of actin patches and loss of tip-located actin cables. These enlarged tips can repolarize and resume hyphal tip extension in the previous polarity axis. AgBoi1/2p permanently localizes to hyphal tips and transiently to sites of septation. Only the tip localization is important for sustained elongation of hyphae. In a yeast two-hybrid experiment, we identified the Rho-type GTPase AgRho3p as an interactor of AgBoi1/2p. AgRho3p is also required to prevent nonpolar growth at hyphal tips, and strains deleted for both AgBOI1/2 and AgRHO3 phenocopied the respective single-deletion strains, demonstrating that AgBoi1/2p and AgRho3p function in a common pathway. Monitoring the polarisome of growing hyphae using AgSpa2p fused to the green fluorescent protein as a marker, we found that polarisome disassembly precedes the onset of nonpolar growth in strains lacking AgBoi1/2p or AgRho3p. AgRho3p locked in its GTP-bound form interacts with the Rho-binding domain of the polarisome-associated formin AgBni1p, implying that AgRho3p has the capacity to directly activate formin-driven actin cable nucleation. We conclude that AgBoi1/2p and AgRho3p support polarisome-mediated actin cable formation at hyphal tips, thereby ensuring permanent polar tip growth. PMID:16950929

  18. Enhanced hyphal growth of arbuscular mycorrhizae by root exudates derived from high R/FR treated Lotus japonicus.

    PubMed

    Nagata, Maki; Yamamoto, Naoya; Miyamoto, Taro; Shimomura, Aya; Arima, Susumu; Hirsch, Ann M; Suzuki, Akihiro

    2016-06-01

    Red/Far Red (R/FR) sensing positively influences the arbuscular mycorrhizal (AM) symbiosis of both legume and nonlegume plants through jasmonic acid (JA) and strigolactone signaling. We previously reported that root exudates obtained from high R/FR-grown plants contained more strigolactone than low R/FR-grown plants. To determine whether JA and JA derivatives were secreted from roots, we investigated the expression levels of JA-responsive genes in L. japonicus Miyakojima MG20 plants treated with root exudates prepared from either high or low R/FR light-treated plants. The root exudates from high R/FR light-treated plants were found to enhance the expression levels of JA-responsive genes significantly. Moreover, exogenous JA increased AM fungal hyphal elongation as did the root exudates derived from high R/FR-grown L. japonicus plants. We conclude that increased JA accumulation and secretion into root exudates from high R/FR light-grown plants is the best explanation for increased colonization and enhanced mycorrhization under these conditions. PMID:27191935

  19. A Model for Growth of a Single Fungal Hypha Based on Well-Mixed Tanks in Series: Simulation of Nutrient and Vesicle Transport in Aerial Reproductive Hyphae

    PubMed Central

    Balmant, Wellington; Sugai-Guérios, Maura Harumi; Coradin, Juliana Hey; Krieger, Nadia; Furigo Junior, Agenor; Mitchell, David Alexander

    2015-01-01

    Current models that describe the extension of fungal hyphae and development of a mycelium either do not describe the role of vesicles in hyphal extension or do not correctly describe the experimentally observed profile for distribution of vesicles along the hypha. The present work uses the n-tanks-in-series approach to develop a model for hyphal extension that describes the intracellular transport of nutrient to a sub-apical zone where vesicles are formed and then transported to the tip, where tip extension occurs. The model was calibrated using experimental data from the literature for the extension of reproductive aerial hyphae of three different fungi, and was able to describe different profiles involving acceleration and deceleration of the extension rate. A sensitivity analysis showed that the supply of nutrient to the sub-apical vesicle-producing zone is a key factor influencing the rate of extension of the hypha. Although this model was used to describe the extension of a single reproductive aerial hypha, the use of the n-tanks-in-series approach to representing the hypha means that the model has the flexibility to be extended to describe the growth of other types of hyphae and the branching of hyphae to form a complete mycelium. PMID:25785863

  20. A model for growth of a single fungal hypha based on well-mixed tanks in series: simulation of nutrient and vesicle transport in aerial reproductive hyphae.

    PubMed

    Balmant, Wellington; Sugai-Guérios, Maura Harumi; Coradin, Juliana Hey; Krieger, Nadia; Furigo Junior, Agenor; Mitchell, David Alexander

    2015-01-01

    Current models that describe the extension of fungal hyphae and development of a mycelium either do not describe the role of vesicles in hyphal extension or do not correctly describe the experimentally observed profile for distribution of vesicles along the hypha. The present work uses the n-tanks-in-series approach to develop a model for hyphal extension that describes the intracellular transport of nutrient to a sub-apical zone where vesicles are formed and then transported to the tip, where tip extension occurs. The model was calibrated using experimental data from the literature for the extension of reproductive aerial hyphae of three different fungi, and was able to describe different profiles involving acceleration and deceleration of the extension rate. A sensitivity analysis showed that the supply of nutrient to the sub-apical vesicle-producing zone is a key factor influencing the rate of extension of the hypha. Although this model was used to describe the extension of a single reproductive aerial hypha, the use of the n-tanks-in-series approach to representing the hypha means that the model has the flexibility to be extended to describe the growth of other types of hyphae and the branching of hyphae to form a complete mycelium.

  1. Heptahelical Receptors GprC and GprD of Aspergillus fumigatus Are Essential Regulators of Colony Growth, Hyphal Morphogenesis, and Virulence▿ †

    PubMed Central

    Gehrke, Alexander; Heinekamp, Thorsten; Jacobsen, Ilse D.; Brakhage, Axel A.

    2010-01-01

    The filamentous fungus Aspergillus fumigatus normally grows on compost or hay but is also able to colonize environments such as the human lung. In order to survive, this organism needs to react to a multitude of external stimuli. Although extensive work has been carried out to investigate intracellular signal transduction in A. fumigatus, little is known about the specific stimuli and the corresponding receptors activating these signaling cascades. Here, two putative G-protein-coupled receptors, GprC and GprD, were characterized with respect to their cellular functions. Deletion of the corresponding genes resulted in drastic growth defects as hyphal extension was reduced, germination was retarded, and hyphae showed elevated levels of branching. The growth defect was found to be temperature dependent. The higher the temperature the more pronounced was the growth defect. Furthermore, compared with the wild type, the sensitivity of the mutant strains toward environmental stress caused by reactive oxygen intermediates was increased and the mutants displayed an attenuation of virulence in a murine infection model. Both mutants, especially the ΔgprC strain, exhibited increased tolerance toward cyclosporine, an inhibitor of the calcineurin signal transduction pathway. Transcriptome analyses indicated that in both the gprC and gprD deletion mutants, transcripts of primary metabolism genes were less abundant, whereas transcription of several secondary metabolism gene clusters was upregulated. Taken together, our data suggest the receptors are involved in integrating and processing stress signals via modulation of the calcineurin pathway. PMID:20418440

  2. Aspergillus nidulans class V and VI chitin synthases CsmA and CsmB, each with a myosin motor-like domain, perform compensatory functions that are essential for hyphal tip growth.

    PubMed

    Takeshita, Norio; Yamashita, Shuichi; Ohta, Akinori; Horiuchi, Hiroyuki

    2006-03-01

    The polarized synthesis of cell wall components such as chitin is essential for the hyphal tip growth of filamentous fungi. The actin cytoskeleton is known to play important roles in the determination of hyphal polarity in Aspergillus nidulans. Previously, we suggested that CsmA, a chitin synthase with a myosin motor-like domain (MMD), was involved in polarized chitin synthesis in a manner dependent on the interaction between the MMD and the actin cytoskeleton. The genome database indicates that A. nidulans possesses another gene encoding another chitin synthase with an MMD. In this study, we characterized this gene, which we designated csmB. The csmB null mutants examined were viable, although they exhibited defective phenotypes, including the formation of balloons and intrahyphal hyphae and the lysis of subapical regions, which were similar to those obtained with csmA null mutants. Moreover, csmA csmB double null mutants were not viable. Mutants in which csmB was deleted and the expression of csmA was under the control of the alcA promoter were viable but severely impaired in terms of hyphal growth under alcA-repressing conditions. We revealed that CsmB with three copies of a FLAG epitope tag localized at the hyphal tips and forming septa, and that the MMD of CsmB was able to bind to actin filaments in vitro. These results suggest that CsmA and CsmB perform compensatory functions that are essential for hyphal tip growth.

  3. Influence of Butylated Hydroxyanisole on the Growth, Hyphal Morphology, and the Biosynthesis of Fumonisins in Fusarium proliferatum.

    PubMed

    Li, Taotao; Jian, Qijie; Chen, Feng; Wang, Yong; Gong, Liang; Duan, Xuewu; Yang, Bao; Jiang, Yueming

    2016-01-01

    Fusarium proliferatum as a common fungus pathogen in foods can produce toxic fumonisins, which can cause animal diseases and increase risks of human cancers. On contrary, butylated hydroxyanisole (BHA) as a synthetic antioxidant offers a clue for preventing growth of fungal species and inhibiting production of mycotoxins. Unfortunately, information of the inhibitory mechanism of BHA on Fusarium species is still limited. In this study, influence of BHA treatment on growth and inhibition of fumonisin production in relation to the expression of the fumonisin biosynthesis-related genes of the F. proliferatum ZYF was investigated, which revealed that BHA had a negative influence on growth and fumonisin production of F. proliferatum. To further elucidate the mechanism of BHA on the growth of F. proliferatum, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to examine the F. proliferatum hyphae. The BHA treatment induced the loss of cytoplasm and cellular constituents, as well as distortion of mycelia, but it did not directly degrade the fumonisin. Furthermore, the BHA treatment markedly inhibited the expressions of FUM1 (a polyketide synthase encoding gene) and FUM8 (an aminotransferase encoding gene) genes, which resulted in the depression of metabolic pathway of F. proliferatum. The transcriptional analyses of the FUM1 and FUM8 genes confirmed a correlation between the fumonisin production and its gene expression. This study provided some insights into mechanisms of production of fumonisin and feasible prevention to reduce fumonisin contamination in favor of human and animal health. PMID:27468276

  4. Influence of Butylated Hydroxyanisole on the Growth, Hyphal Morphology, and the Biosynthesis of Fumonisins in Fusarium proliferatum

    PubMed Central

    Li, Taotao; Jian, Qijie; Chen, Feng; Wang, Yong; Gong, Liang; Duan, Xuewu; Yang, Bao; Jiang, Yueming

    2016-01-01

    Fusarium proliferatum as a common fungus pathogen in foods can produce toxic fumonisins, which can cause animal diseases and increase risks of human cancers. On contrary, butylated hydroxyanisole (BHA) as a synthetic antioxidant offers a clue for preventing growth of fungal species and inhibiting production of mycotoxins. Unfortunately, information of the inhibitory mechanism of BHA on Fusarium species is still limited. In this study, influence of BHA treatment on growth and inhibition of fumonisin production in relation to the expression of the fumonisin biosynthesis-related genes of the F. proliferatum ZYF was investigated, which revealed that BHA had a negative influence on growth and fumonisin production of F. proliferatum. To further elucidate the mechanism of BHA on the growth of F. proliferatum, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to examine the F. proliferatum hyphae. The BHA treatment induced the loss of cytoplasm and cellular constituents, as well as distortion of mycelia, but it did not directly degrade the fumonisin. Furthermore, the BHA treatment markedly inhibited the expressions of FUM1 (a polyketide synthase encoding gene) and FUM8 (an aminotransferase encoding gene) genes, which resulted in the depression of metabolic pathway of F. proliferatum. The transcriptional analyses of the FUM1 and FUM8 genes confirmed a correlation between the fumonisin production and its gene expression. This study provided some insights into mechanisms of production of fumonisin and feasible prevention to reduce fumonisin contamination in favor of human and animal health. PMID:27468276

  5. Hyphal growth and mycorrhiza formation by the arbuscular mycorrhizal fungus Glomus claroideum BEG 23 is stimulated by humic substances.

    PubMed

    Gryndler, M; Hrselová, H; Sudová, R; Gryndlerová, H; Rezácová, V; Merhautová, V

    2005-11-01

    Effects of humic substances (humic acid or fulvic soil extract) or saprophytic microorganisms (Paecilomyces lilacinus and an unidentified actinomycete) on growth of mycelium and mycorrhiza formation by Glomus claroideum BEG23 were studied in a hydroponic system. Humic substances stimulated root colonization and production of extraradical mycelium by the mycorrhizal fungus. Both humic and fulvic acids tended to decrease populations of culturable bacteria and fungi in the cultivation system, indicating a moderately antibiotic activity. The addition of saprophytic microorganisms able to use humic substances to the cultivation system further stimulated the development of the mycorrhizal fungus. However, stimulation of G. claroideum was also observed when the saprophytic microorganisms were heat-killed, suggesting that their effect was not linked to a specific action on humic substances. The results indicate that humic substances may represent a stimulatory component of the soil environment with respect to arbuscular mycorrhizal fungi.

  6. WdStuAp, an APSES Transcription Factor, Is a Regulator of Yeast-Hyphal Transitions in Wangiella (Exophiala) dermatitidis▿ †

    PubMed Central

    Wang, Qin; Szaniszlo, Paul J.

    2007-01-01

    APSES transcription factors are well-known regulators of fungal cellular development and differentiation. To study the function of an APSES protein in the fungus Wangiella dermatitidis, a conidiogenous and polymorphic agent of human phaeohyphomycosis with yeast predominance, the APSES transcription factor gene WdSTUA was cloned, sequenced, disrupted, and overexpressed. Analysis showed that its derived protein was most similar to the APSES proteins of other conidiogenous molds and had its APSES DNA-binding domain located in the amino-terminal half. Deletion of WdSTUA in W. dermatitidis induced convoluted instead of normal smooth colony surface growth on the rich yeast maintenance agar medium yeast extract-peptone-dextrose agar (YPDA) at 37°C. Additionally, deletion of WdSTUA repressed aerial hyphal growth, conidiation, and invasive hyphal growth on the nitrogen-poor, hypha-inducing agar medium potato dextrose agar (PDA) at 25°C. Ectopic overexpression of WdSTUA repressed the convoluted colony surface growth on YPDA at 37°C, and also strongly repressed hyphal growth on PDA at 25°C and 37°C. These new results provide additional insights into the diverse roles played by APSES factors in fungi. They also suggest that the transcription factor encoded by WdSTUA is both a positive and negative morphotype regulator in W. dermatitidis and possibly other of the numerous human pathogenic, conidiogenous fungi capable of yeast growth. PMID:17693595

  7. The transcription factor Ste12 mediates the regulatory role of the Tmk1 MAP kinase in mycoparasitism and vegetative hyphal fusion in the filamentous fungus Trichoderma atroviride.

    PubMed

    Gruber, Sabine; Zeilinger, Susanne

    2014-01-01

    Mycoparasitic species of the fungal genus Trichoderma are potent antagonists able to combat plant pathogenic fungi by direct parasitism. An essential step in this mycoparasitic fungus-fungus interaction is the detection of the fungal host followed by activation of molecular weapons in the mycoparasite by host-derived signals. The Trichoderma atroviride MAP kinase Tmk1, a homolog of yeast Fus3/Kss1, plays an essential role in regulating the mycoparasitic host attack, aerial hyphae formation and conidiation. However, the transcription factors acting downstream of Tmk1 are hitherto unknown. Here we analyzed the functions of the T. atroviride Ste12 transcription factor whose orthologue in yeast is targeted by the Fus3 and Kss1 MAP kinases. Deletion of the ste12 gene in T. atroviride not only resulted in reduced mycoparasitic overgrowth and lysis of host fungi but also led to loss of hyphal avoidance in the colony periphery and a severe reduction in conidial anastomosis tube formation and vegetative hyphal fusion events. The transcription of several orthologues of Neurospora crassa hyphal fusion genes was reduced upon ste12 deletion; however, the Δste12 mutant showed enhanced expression of mycoparasitism-relevant chitinolytic and proteolytic enzymes and of the cell wall integrity MAP kinase Tmk2. Based on the comparative analyses of Δste12 and Δtmk1 mutants, an essential role of the Ste12 transcriptional regulator in mediating outcomes of the Tmk1 MAPK pathway such as regulation of the mycoparasitic activity, hyphal fusion and carbon source-dependent vegetative growth is suggested. Aerial hyphae formation and conidiation, in contrast, were found to be independent of Ste12.

  8. The transcription factor Ste12 mediates the regulatory role of the Tmk1 MAP kinase in mycoparasitism and vegetative hyphal fusion in the filamentous fungus Trichoderma atroviride.

    PubMed

    Gruber, Sabine; Zeilinger, Susanne

    2014-01-01

    Mycoparasitic species of the fungal genus Trichoderma are potent antagonists able to combat plant pathogenic fungi by direct parasitism. An essential step in this mycoparasitic fungus-fungus interaction is the detection of the fungal host followed by activation of molecular weapons in the mycoparasite by host-derived signals. The Trichoderma atroviride MAP kinase Tmk1, a homolog of yeast Fus3/Kss1, plays an essential role in regulating the mycoparasitic host attack, aerial hyphae formation and conidiation. However, the transcription factors acting downstream of Tmk1 are hitherto unknown. Here we analyzed the functions of the T. atroviride Ste12 transcription factor whose orthologue in yeast is targeted by the Fus3 and Kss1 MAP kinases. Deletion of the ste12 gene in T. atroviride not only resulted in reduced mycoparasitic overgrowth and lysis of host fungi but also led to loss of hyphal avoidance in the colony periphery and a severe reduction in conidial anastomosis tube formation and vegetative hyphal fusion events. The transcription of several orthologues of Neurospora crassa hyphal fusion genes was reduced upon ste12 deletion; however, the Δste12 mutant showed enhanced expression of mycoparasitism-relevant chitinolytic and proteolytic enzymes and of the cell wall integrity MAP kinase Tmk2. Based on the comparative analyses of Δste12 and Δtmk1 mutants, an essential role of the Ste12 transcriptional regulator in mediating outcomes of the Tmk1 MAPK pathway such as regulation of the mycoparasitic activity, hyphal fusion and carbon source-dependent vegetative growth is suggested. Aerial hyphae formation and conidiation, in contrast, were found to be independent of Ste12. PMID:25356841

  9. The Transcription Factor Ste12 Mediates the Regulatory Role of the Tmk1 MAP Kinase in Mycoparasitism and Vegetative Hyphal Fusion in the Filamentous Fungus Trichoderma atroviride

    PubMed Central

    Gruber, Sabine; Zeilinger, Susanne

    2014-01-01

    Mycoparasitic species of the fungal genus Trichoderma are potent antagonists able to combat plant pathogenic fungi by direct parasitism. An essential step in this mycoparasitic fungus-fungus interaction is the detection of the fungal host followed by activation of molecular weapons in the mycoparasite by host-derived signals. The Trichoderma atroviride MAP kinase Tmk1, a homolog of yeast Fus3/Kss1, plays an essential role in regulating the mycoparasitic host attack, aerial hyphae formation and conidiation. However, the transcription factors acting downstream of Tmk1 are hitherto unknown. Here we analyzed the functions of the T. atroviride Ste12 transcription factor whose orthologue in yeast is targeted by the Fus3 and Kss1 MAP kinases. Deletion of the ste12 gene in T. atroviride not only resulted in reduced mycoparasitic overgrowth and lysis of host fungi but also led to loss of hyphal avoidance in the colony periphery and a severe reduction in conidial anastomosis tube formation and vegetative hyphal fusion events. The transcription of several orthologues of Neurospora crassa hyphal fusion genes was reduced upon ste12 deletion; however, the Δste12 mutant showed enhanced expression of mycoparasitism-relevant chitinolytic and proteolytic enzymes and of the cell wall integrity MAP kinase Tmk2. Based on the comparative analyses of Δste12 and Δtmk1 mutants, an essential role of the Ste12 transcriptional regulator in mediating outcomes of the Tmk1 MAPK pathway such as regulation of the mycoparasitic activity, hyphal fusion and carbon source-dependent vegetative growth is suggested. Aerial hyphae formation and conidiation, in contrast, were found to be independent of Ste12. PMID:25356841

  10. Hyphal formation of Candida albicans is controlled by electron transfer system

    SciTech Connect

    Watanabe, Toshihiko . E-mail: twatanab@tohoku-pharm.ac.jp; Ogasawara, Ayako; Mikami, Takeshi; Matsumoto, Tatsuji

    2006-09-15

    Most Candida albicans cells cultured in RPMI1640 medium at 37 deg. C grow in hyphal form in aerobic conditions, but they grow in yeast form in anaerobic conditions. The hyphal growth of C. albicans was inhibited in glucose-deficient conditions. Malonic acid, an inhibitor of succinate dehydrogenase, enhanced the yeast proliferation of C. albicans, indicating that the hyphal-formation signal was derived from the glycolysis system and the signal was transmitted to the electron transfer system via the citric acid cycle. Thenoyl trifluoro acetone (TTFA), an inhibitor of the signal transmission between complex II and Co Q, significantly inhibited the hyphal growth of C. albicans. Antimycin, KCN, and oligomycin, inhibitors of complex III, IV, and V, respectively, did not inhibit the hyphal growth of C. albicans. The production of mRNAs for the hyphal formation signal was completely inhibited in anaerobic conditions. These results indicate that the electron transfer system functions upstream of the RAS1 signal pathway and activates the expression of the hyphal formation signal. Since the electron transfer system is inactivated in anaerobic conditions, C. albicans grew in yeast form in this condition.

  11. Gene encoding a C-type cyclin in Mycosphaerella graminicola is involved in aerial mycelium formation, filamentous growth, hyphal swelling, melanin biosynthesis, stress response, and pathogenicity

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Mycosphaerella graminicola is an important wheat pathogen causing septoria tritici blotch. To date, an efficient strategy to control M. graminicola has not been developed. More significantly, we have a limited understanding of the molecular mechanisms of M. graminicola pathogenicity. In this study, ...

  12. Canine intestinal histoplasmosis containing hyphal forms.

    PubMed

    Schumacher, Loni L; Love, Brenda C; Ferrell, Mark; DeSilva, Udaya; Fernando, Ruchika; Ritchey, Jerry W

    2013-03-01

    A 12-year-old intact male Miniature Schnauzer dog with chronic diarrhea that was unresponsive to empirical treatment was presented to a referring veterinarian. A laparotomy was performed, and formalin-fixed biopsies of duodenum, jejunum, and colon were sent to Oklahoma Animal Disease Diagnostic Laboratory for evaluation. Histologic examination revealed a severe, diffuse, granulomatous enteritis and colitis with intralesional yeast and hyphal forms. Grocott methenamine silver stains revealed short, aseptate hyphae co-mingled with 2-8 µm, oval to round yeast organisms consistent with Histoplasma capsulatum. The atypical presentation of both yeast and hyphal forms prompted identification of the organism. Direct sequencing of a polymerase chain reaction product from paraffin-embedded intestinal samples confirmed the presence of Ajellomyces capsulatus with a homology over 99% to several sequences in GenBank. Ajellomyces capsulatus is the holomorphic name for H. capsulatum. Therefore, the mycelial form of a dimorphic fungus such as H. capsulatum can coexist with yeast cells within lesions of histoplasmosis. Following diagnosis, the dog was treated with itraconazole for 6 months and has improved. PMID:23512926

  13. High-Resolution Monitoring of Coastal Dune Erosion and Growth Using an Unmanned Aerial Vehicle

    NASA Astrophysics Data System (ADS)

    Ruessink, G.; Markies, H.; Van Maarseveen, M.

    2014-12-01

    Coastal foredunes lose and gain sand through marine and aeolian processes, but coastal-evolution models that can accurately predict both wave-driven dune erosion and wind-blown dune growth are non-existing. This is, together with a limited understanding of coastal aeolian process dynamics, due to the lack of adequate field data sets from which erosion and supply volumes can be studied simultaneously. Here, we quantify coastal foredune dynamics using nine topographic surveys performed near Egmond aan Zee, The Netherlands, between September 2011 and March 2014 using an unmanned aerial vehicle (UAV). The approximately 0.75-km long study site comprises a 30-100 m wide sandy beach and a 20-25 m high foredune, of which the higher parts are densely vegetated with European marram grass. Using a structure-from-motion workflow, the 200-500 photographs taken during each UAV flight were processed into a point cloud, from which a geo-referenced digital surface model with a 0.25 x 0.25 m resolution was subsequently computed. Our data set contains two dune-erosion events, including that due to storm Xaver (December 2013), which caused one of the highest surge levels in the southern North Sea region for the last decades. Dune erosion during both events varied alongshore from the destruction of embryonic dunes on the upper beach to the slumping of the entire dune face. During the first storm (January 2012), erosion volumes ranged from 5 m3/m in the (former) embryonic dune field to over 40 m3/m elsewhere. During the subsequent 11 (spring - autumn) months, the foredune accreted by (on average) 8 m3/m, again with substantial alongshore variability (0 - 20 m3/m). Intriguingly, volume changes during the 2012-2013 winter were minimal. We will compare the observed aeolian supply rates with model predictions and discuss reasons for their temporal variability. Funded by the Dutch Organisation for Scientific Research NWO.

  14. Uniformity of environmental conditions and plant growth in a hydroponic culture system for use in a growth room with aerial CO2 control

    NASA Technical Reports Server (NTRS)

    Vessey, J. K.; York, E. K.; Henry, L. T.; Raper, C. D. Jr; Raper CD, J. r. (Principal Investigator)

    1988-01-01

    A portable system of hydroponic culture was developed that maintained temperature, pH, and nutrient concentrations of circulating nutrient solutions. The hydroponic system is used within a controlled-environment room (CER) for control of aerial environment. The CER was equipped with an auto-calibrating system for atmospheric CO2 control. The control systems for the hydroponic chambers were able to maintain acidity within +/- 0.2 pH units and the temperature with +/- 0.5 degree C. Mixing time for the 200-liter volume of solution within a hydroponic chamber was less than 12 min. The CO2 control system was able to maintain aerial concentrations within +/- 10 ppm CO2 during the light period. The only gradient found to occur within the hydroponic chambers or CER was a slight gradient in aerial temperature along the length of hydroponic chambers. Growth of soybeans [Glycine max (L.) Merr.] was characterized during a 3-week period of vegetative development by leaf number and area, plant dry weight, total N content of plants, and N depletion from the nutrient solution. The growth characteristics among populations for three hydroponic chambers within the CER were not significantly different, and the percent standard errors of means of the measurements within populations from each chamber were nearly all less than 10%. Thus, the uniformity of plant growth reflected the uniformity of environmental conditions.

  15. SclR, a Basic Helix-Loop-Helix Transcription Factor, Regulates Hyphal Morphology and Promotes Sclerotial Formation in Aspergillus oryzae ▿ †

    PubMed Central

    Jin, Feng Jie; Takahashi, Tadashi; Matsushima, Ken-ichiro; Hara, Seiichi; Shinohara, Yasutomo; Maruyama, Jun-ichi; Kitamoto, Katsuhiko; Koyama, Yasuji

    2011-01-01

    Most known basic-region helix-loop-helix (bHLH) proteins belong to a superfamily of transcription factors often involved in the control of growth and differentiation. Therefore, inappropriate expression of genes encoding bHLH proteins is frequently associated with developmental dysfunction. In our previously reported study, a novel bHLH protein-encoding gene (AO090011000215) of Aspergillus oryzae was identified. The gene-disrupted strain was found to produce dense conidia, but sparse sclerotia, relative to the parent strain. Here, to further analyze its function, we generated an overexpressing strain using the A. oryzae amyB gene promoter. Genetic overexpression led to a large number of initial hyphal aggregations and then the formation of mature sclerotia; it was therefore designated sclR (sclerotium regulator). At the same time, the sclR-overexpressing strain also displayed both delayed and decreased conidiation. Scanning electron microscopy indicated that the aerial hyphae of the sclR-overexpressing strain were extremely branched and intertwined with each other. In the generation of the SclR-enhanced green fluorescent protein (EGFP) expression strain, the SclR-EGFP protein fusion was conditionally detected in the nuclei. In addition, the loss of sclR function led to rapid protein degradation and cell lysis in dextrin-polypeptone-yeast extract liquid medium. Taken together, these observations indicate that SclR plays an important role in hyphal morphology, asexual conidiospore formation, and the promotion of sclerotial production, even retaining normal cell function, at least in submerged liquid culture. PMID:21551246

  16. A circadian rhythm regulating hyphal melanization in Cercospora kikuchii.

    PubMed

    Bluhm, Burton H; Burnham, A Michele; Dunkle, Larry D

    2010-01-01

    Many metabolic and developmental processes in fungi are controlled by biological rhythms. Circadian rhythms approximate a daily (24 h) cycle and have been thoroughly studied in the model fungus, Neurospora crassa. However relatively few examples of true circadian rhythms have been documented among other filamentous fungi. In this study we describe a circadian rhythm underlying hyphal melanization in Cercospora kikuchii, an important pathogen of soybean. After growth in light or light : dark cycles, colonies transferred to darkness produced zonate bands of melanized hyphae interspersed with bands of hyaline hyphae. Rhythmic production of bands was remarkably persistent in the absence of external cues, lasting at least 7 d after transfer to darkness, and was compensated over a range of temperatures. As in N. crassa, blue light but not red light was sufficient to entrain the circadian rhythm in C. kikuchii, and a putative ortholog of white collar-1, one of the genes required for light responses in N. crassa, was identified in C. kikuchii. Circadian regulation of melanization is conserved in other members of the genus: Similar rhythms were identified in another field isolate of C. kikuchii as well as field isolates of C. beticola and C. sorghi, but not in wild-type strains of C. zeae-maydis or C. zeina. This report represents the first documented circadian rhythm among Dothideomycete fungi and provides a new opportunity to dissect the molecular basis of circadian rhythms among filamentous fungi.

  17. A circadian rhythm regulating hyphal melanization in Cercospora kikuchii.

    PubMed

    Bluhm, Burton H; Burnham, A Michele; Dunkle, Larry D

    2010-01-01

    Many metabolic and developmental processes in fungi are controlled by biological rhythms. Circadian rhythms approximate a daily (24 h) cycle and have been thoroughly studied in the model fungus, Neurospora crassa. However relatively few examples of true circadian rhythms have been documented among other filamentous fungi. In this study we describe a circadian rhythm underlying hyphal melanization in Cercospora kikuchii, an important pathogen of soybean. After growth in light or light : dark cycles, colonies transferred to darkness produced zonate bands of melanized hyphae interspersed with bands of hyaline hyphae. Rhythmic production of bands was remarkably persistent in the absence of external cues, lasting at least 7 d after transfer to darkness, and was compensated over a range of temperatures. As in N. crassa, blue light but not red light was sufficient to entrain the circadian rhythm in C. kikuchii, and a putative ortholog of white collar-1, one of the genes required for light responses in N. crassa, was identified in C. kikuchii. Circadian regulation of melanization is conserved in other members of the genus: Similar rhythms were identified in another field isolate of C. kikuchii as well as field isolates of C. beticola and C. sorghi, but not in wild-type strains of C. zeae-maydis or C. zeina. This report represents the first documented circadian rhythm among Dothideomycete fungi and provides a new opportunity to dissect the molecular basis of circadian rhythms among filamentous fungi. PMID:20943572

  18. Recent Growth of Aerial Photographic Interpretation/Remote Sensing in Geography in the United States

    ERIC Educational Resources Information Center

    Estes, John E.; Thaman, Konai

    1974-01-01

    This paper traces the history and growth of air photo interpretation and remote sensing within the field of geography. Courses offered in these fields, factors influencing growth, research findings, and professional geographic interest are discussed. (JH)

  19. FgKin1 kinase localizes to the septal pore and plays a role in hyphal growth, ascospore germination, pathogenesis, and localization of Tub1 beta-tubulins in Fusarium graminearum.

    PubMed

    Luo, Yongping; Zhang, Hongchang; Qi, Linlu; Zhang, Shijie; Zhou, Xiaoying; Zhang, Yimei; Xu, Jin-Rong

    2014-12-01

    The Kin1/Par-1/MARK kinases regulate various cellular processes in eukaryotic organisms. Kin1 orthologs are well conserved in fungal pathogens but none of them have been functionally characterized. Here, we show that KIN1 is important for pathogenesis and growth in two phytopathogenic fungi and that FgKin1 regulates ascospore germination and the localization of Tub1 β-tubulins in Fusarium graminearum. The Fgkin1 mutant and putative FgKIN1(S172A) kinase dead (nonactivatable) transformants were characterized for defects in plant infection, sexual and asexual reproduction, and stress responses. The localization of FgKin1 and two β-tubulins were examined in the wild-type and mutant backgrounds. Deletion of FgKIN1 resulted in reduced virulence and defects in ascospore germination and release. FgKin1 localized to the center of septal pores. FgKIN1 deletion had no effect on Tub2 microtubules but disrupted Tub1 localization. In the mutant, Tub1 appeared to be enriched in the nucleolus. In Magnaporthe oryzae, MoKin1 has similar functions in growth and infection and it also localizes to septal pores. The S172A mutation had no effect on the localization and function of FgKIN1 during sexual reproduction. These results indicate that FgKIN1 has kinase-dependent and independent functions and it specifically regulates Tub1 β-tubulins. FgKin1 plays a critical role in ascospore discharge, germination, and plant infection. PMID:25078365

  20. Coccidioides immitis presenting as a hyphal form in cerebrospinal fluid.

    PubMed Central

    Zepeda, M. R.; Kobayashi, G. K.; Appleman, M. D.; Navarro, A.

    1998-01-01

    This article reports a case of Coccidioides immitis that presented as a hyphal form in a 38-year-old patient. The organism was observed growing exclusively as hyphae in the cerebrospinal fluid by microscopic examination. Coccidioides immitis was the only organism cultured. The identification of C immitis was confirmed by both standard culture methods and DNA probe studies. Images Figure PMID:9685779

  1. Aerial Explorers

    NASA Technical Reports Server (NTRS)

    Young, Larry A.; Pisanich, Greg; Ippolito, Corey

    2005-01-01

    This paper presents recent results from a mission architecture study of planetary aerial explorers. In this study, several mission scenarios were developed in simulation and evaluated on success in meeting mission goals. This aerial explorer mission architecture study is unique in comparison with previous Mars airplane research activities. The study examines how aerial vehicles can find and gain access to otherwise inaccessible terrain features of interest. The aerial explorer also engages in a high-level of (indirect) surface interaction, despite not typically being able to takeoff and land or to engage in multiple flights/sorties. To achieve this goal, a new mission paradigm is proposed: aerial explorers should be considered as an additional element in the overall Entry, Descent, Landing System (EDLS) process. Further, aerial vehicles should be considered primarily as carrier/utility platforms whose purpose is to deliver air-deployed sensors and robotic devices, or symbiotes, to those high-value terrain features of interest.

  2. Monoclonal Antibodies to Hyphal Exoantigens Derived from the Opportunistic Pathogen Aspergillus terreus ▿

    PubMed Central

    Nayak, Ajay P.; Green, Brett J.; Janotka, Erika; Hettick, Justin M.; Friend, Sherri; Vesper, Steve J.; Schmechel, Detlef; Beezhold, Donald H.

    2011-01-01

    Aspergillus terreus has been difficult to identify in cases of aspergillosis, and clinical identification has been restricted to the broad identification of aspergillosis lesions in affected organs or the detection of fungal carbohydrates. As a result, there is a clinical need to identify species-specific biomarkers that can be used to detect invasive A. terreus disease. Monoclonal antibodies (MAbs) were developed to a partially purified preparation of cytolytic hyphal exoantigens (HEA) derived from A. terreus culture supernatant (CSN). Twenty-three IgG1 isotype murine MAbs were developed and tested for cross-reactivity against hyphal extracts of 54 fungal species. Sixteen MAbs were shown to be specific for A. terreus. HEA were detected in conidia, hyphae, and in CSN of A. terreus. HEA were expressed in high levels in the hyphae during early stages of A. terreus growth at 37°C, whereas at room temperature the expression of HEA peaked by days 4 to 5. Expression kinetics of HEA in CSN showed a lag, with peak levels at later time points at room temperature and 37°C than in hyphal extracts. Serum spiking experiments demonstrated that human serum components do not inhibit detection of the HEA epitopes by MAb enzyme-linked immunosorbent assay (ELISA). Immunoprecipitation and proteomic analysis demonstrated that MAbs 13E11 and 12C4 immunoprecipitated a putative uncharacterized leucine aminopeptidase (Q0CAZ7), while MAb 19B2 recognized a putative dipeptidyl-peptidase V (DPP5). Studies using confocal laser scanning microscopy showed that the uncharacterized leucine aminopeptidase mostly localized to extracellular matrix structures while dipeptidyl-peptidase V was mostly confined to the cytoplasm. PMID:21734068

  3. Mutations Affecting Hyphal Colonization and Pyoverdine Production in Pseudomonads Antagonistic toward Phytophthora parasitica.

    PubMed

    Yang, C H; Menge, J A; Cooksey, D A

    1994-02-01

    In previous studies, Pseudomonas putida 06909 and Pseudomonas fluorescens 09906 suppressed populations of Phytophthora parasitica in the citrus rhizosphere, suggesting that these bacteria may be useful in biological control of citrus root rot. In this study we investigated the mechanisms of antagonism between the bacteria and the fungus. Both bacteria colonized Phytophthora hyphae and inhibited the fungus on agar media. A hyphal column assay was developed to measure the colonization of bacteria on fungal hyphae and to enrich for colonization-deficient mutants. In this way we identified Tn5 mutants of each pseudomonad that were not able to colonize the hyphae and inhibit fungal growth in vitro. Colonization-deficient mutants were nonmotile and lacked flagella. Survival of nonmotile mutants in a citrus soil was similar to survival of a random Tn5 mutant over a 52-day period. Additional screening of random Tn5 mutants of both pseudomonads for loss of fungal inhibition in vitro yielded two distinct types of mutants. Mutants of the first type were deficient in production of pyoverdines and in inhibition of the fungus in vitro, although they still colonized fungal hyphae. Mutants of the second type lacked flagella and were not able to colonize the hyphae or inhibit fungal growth. No role was found for antibiotic production by the two bacteria in the inhibition of the fungus. Our results suggest that both hyphal colonization and pyoverdine production are important in the inhibition of Phytophthora parasitica by P. fluorescens and P. putida in vitro. PMID:16349177

  4. The hyphal wall of Mucor mucedo. 1. Polyanionic polymers.

    PubMed

    Datema, R; van den Ende, H; Wessels, J G

    1977-11-01

    Treatment of isolated hyphal walls of Mucor mucedo with nitrous acid resulted in the release of two water-soluble polyanions: (a) a glycuronan, containing all the neutral sugars and uronic acid present in the hyphal wall and (b) an inorganic polyphosphate. The glycuronan could also be extracted quantitatively with salt solutions of high ionic strength and partially with a solution of potassium hydroxide. This is presented as evidence that the glycuronan is a genuine constituent of the cell wall, non-covalently bound to glucosamine-containing polymers which are susceptible to depolymerization by nitrous acid. By treatment with acid the glycuronan was partly converted to crystalline poly(glucuronic acid) with the properties of mucoric acid. This strongly suggests that mucoric acid, which can be extracted from the walls of M. mucedo by alkali after acid treatment, is not a genuine wall component but arises by partial acid hydrolysis of the heteropolymeric glycuronan.

  5. Nuclear dynamics during germination, conidiation, and hyphal fusion of Fusarium oxysporum.

    PubMed

    Ruiz-Roldán, M Carmen; Köhli, Michael; Roncero, M Isabel G; Philippsen, Peter; Di Pietro, Antonio; Espeso, Eduardo A

    2010-08-01

    In many fungal pathogens, infection is initiated by conidial germination. Subsequent stages involve germ tube elongation, conidiation, and vegetative hyphal fusion (anastomosis). Here, we used live-cell fluorescence to study the dynamics of green fluorescent protein (GFP)- and cherry fluorescent protein (ChFP)-labeled nuclei in the plant pathogen Fusarium oxysporum. Hyphae of F. oxysporum have uninucleated cells and exhibit an acropetal nuclear pedigree, where only the nucleus in the apical compartment is mitotically active. In contrast, conidiation follows a basopetal pattern, whereby mononucleated microconidia are generated by repeated mitotic cycles of the subapical nucleus in the phialide, followed by septation and cell abscission. Vegetative hyphal fusion is preceded by directed growth of the fusion hypha toward the receptor hypha and followed by a series of postfusion nuclear events, including mitosis of the apical nucleus of the fusion hypha, migration of a daughter nucleus into the receptor hypha, and degradation of the resident nucleus. These previously unreported patterns of nuclear dynamics in F. oxysporum could be intimately related to its pathogenic lifestyle.

  6. A Single Nucleotide Polymorphism Uncovers a Novel Function for the Transcription Factor Ace2 during Candida albicans Hyphal Development

    PubMed Central

    Orellana-Muñoz, Sara; Gutiérrez-Escribano, Pilar; Arnáiz-Pita, Yolanda; Dueñas-Santero, Encarnación; Suárez, M. Belén; Bougnoux, Marie-Elisabeth; del Rey, Francisco; Sherlock, Gavin; d’Enfert, Christophe; Correa-Bordes, Jaime; de Aldana, Carlos R. Vázquez

    2015-01-01

    Candida albicans is a major invasive fungal pathogen in humans. An important virulence factor is its ability to switch between the yeast and hyphal forms, and these filamentous forms are important in tissue penetration and invasion. A common feature for filamentous growth is the ability to inhibit cell separation after cytokinesis, although it is poorly understood how this process is regulated developmentally. In C. albicans, the formation of filaments during hyphal growth requires changes in septin ring dynamics. In this work, we studied the functional relationship between septins and the transcription factor Ace2, which controls the expression of enzymes that catalyze septum degradation. We found that alternative translation initiation produces two Ace2 isoforms. While full-length Ace2, Ace2L, influences septin dynamics in a transcription-independent manner in hyphal cells but not in yeast cells, the use of methionine-55 as the initiation codon gives rise to Ace2S, which functions as the nuclear transcription factor required for the expression of cell separation genes. Genetic evidence indicates that Ace2L influences the incorporation of the Sep7 septin to hyphal septin rings in order to avoid inappropriate activation of cell separation during filamentous growth. Interestingly, a natural single nucleotide polymorphism (SNP) present in the C. albicans WO-1 background and other C. albicans commensal and clinical isolates generates a stop codon in the ninth codon of Ace2L that mimics the phenotype of cells lacking Ace2L. Finally, we report that Ace2L and Ace2S interact with the NDR kinase Cbk1 and that impairing activity of this kinase results in a defect in septin dynamics similar to that of hyphal cells lacking Ace2L. Together, our findings identify Ace2L and the NDR kinase Cbk1 as new elements of the signaling system that modify septin ring dynamics in hyphae to allow cell-chain formation, a feature that appears to have evolved in specific C. albicans lineages

  7. The Endoplasmic Reticulum-Mitochondrion Tether ERMES Orchestrates Fungal Immune Evasion, Illuminating Inflammasome Responses to Hyphal Signals.

    PubMed

    Tucey, Timothy M; Verma-Gaur, Jiyoti; Nguyen, Julie; Hewitt, Victoria L; Lo, Tricia L; Shingu-Vazquez, Miguel; Robertson, Avril A B; Hill, James R; Pettolino, Filomena A; Beddoe, Travis; Cooper, Matthew A; Naderer, Thomas; Traven, Ana

    2016-01-01

    The pathogenic yeast Candida albicans escapes macrophages by triggering NLRP3 inflammasome-dependent host cell death (pyroptosis). Pyroptosis is inflammatory and must be tightly regulated by host and microbe, but the mechanism is incompletely defined. We characterized the C. albicans endoplasmic reticulum (ER)-mitochondrion tether ERMES and show that the ERMES mmm1 mutant is severely crippled in killing macrophages despite hyphal formation and normal phagocytosis and survival. To understand dynamic inflammasome responses to Candida with high spatiotemporal resolution, we established live-cell imaging for parallel detection of inflammasome activation and pyroptosis at the single-cell level. This showed that the inflammasome response to mmm1 mutant hyphae is delayed by 10 h, after which an exacerbated activation occurs. The NLRP3 inhibitor MCC950 inhibited inflammasome activation and pyroptosis by C. albicans, including exacerbated inflammasome activation by the mmm1 mutant. At the cell biology level, inactivation of ERMES led to a rapid collapse of mitochondrial tubular morphology, slow growth and hyphal elongation at host temperature, and reduced exposed 1,3-β-glucan in hyphal populations. Our data suggest that inflammasome activation by C. albicans requires a signal threshold dependent on hyphal elongation and cell wall remodeling, which could fine-tune the response relative to the level of danger posed by C. albicans. The phenotypes of the ERMES mutant and the lack of conservation in animals suggest that ERMES is a promising antifungal drug target. Our data further indicate that NLRP3 inhibition by MCC950 could modulate C. albicans-induced inflammation. IMPORTANCE The yeast Candida albicans causes human infections that have mortality rates approaching 50%. The key to developing improved therapeutics is to understand the host-pathogen interface. A critical interaction is that with macrophages: intracellular Candida triggers the NLRP3/caspase-1 inflammasome

  8. The Endoplasmic Reticulum-Mitochondrion Tether ERMES Orchestrates Fungal Immune Evasion, Illuminating Inflammasome Responses to Hyphal Signals

    PubMed Central

    Tucey, Timothy M.; Verma-Gaur, Jiyoti; Nguyen, Julie; Hewitt, Victoria L.; Lo, Tricia L.; Shingu-Vazquez, Miguel; Robertson, Avril A. B.; Hill, James R.; Pettolino, Filomena A.; Beddoe, Travis; Cooper, Matthew A.; Naderer, Thomas

    2016-01-01

    ABSTRACT The pathogenic yeast Candida albicans escapes macrophages by triggering NLRP3 inflammasome-dependent host cell death (pyroptosis). Pyroptosis is inflammatory and must be tightly regulated by host and microbe, but the mechanism is incompletely defined. We characterized the C. albicans endoplasmic reticulum (ER)-mitochondrion tether ERMES and show that the ERMES mmm1 mutant is severely crippled in killing macrophages despite hyphal formation and normal phagocytosis and survival. To understand dynamic inflammasome responses to Candida with high spatiotemporal resolution, we established live-cell imaging for parallel detection of inflammasome activation and pyroptosis at the single-cell level. This showed that the inflammasome response to mmm1 mutant hyphae is delayed by 10 h, after which an exacerbated activation occurs. The NLRP3 inhibitor MCC950 inhibited inflammasome activation and pyroptosis by C. albicans, including exacerbated inflammasome activation by the mmm1 mutant. At the cell biology level, inactivation of ERMES led to a rapid collapse of mitochondrial tubular morphology, slow growth and hyphal elongation at host temperature, and reduced exposed 1,3-β-glucan in hyphal populations. Our data suggest that inflammasome activation by C. albicans requires a signal threshold dependent on hyphal elongation and cell wall remodeling, which could fine-tune the response relative to the level of danger posed by C. albicans. The phenotypes of the ERMES mutant and the lack of conservation in animals suggest that ERMES is a promising antifungal drug target. Our data further indicate that NLRP3 inhibition by MCC950 could modulate C. albicans-induced inflammation. IMPORTANCE The yeast Candida albicans causes human infections that have mortality rates approaching 50%. The key to developing improved therapeutics is to understand the host-pathogen interface. A critical interaction is that with macrophages: intracellular Candida triggers the NLRP3/caspase-1

  9. Aerial Photography

    NASA Technical Reports Server (NTRS)

    1985-01-01

    John Hill, a pilot and commercial aerial photographer, needed an information base. He consulted NERAC and requested a search of the latest developments in camera optics. NERAC provided information; Hill contacted the manufacturers of camera equipment and reduced his photographic costs significantly.

  10. Influence of environmental conditions on hyphal morphology in pellets of Aspergillus niger: role of beta-N-acetyl-D-glucosaminidase.

    PubMed

    Pera, L M; Baigorí, M D; Callieri, D

    1999-08-01

    The influence of modifications of the environmental conditions of growth on beta-N-acetyl-D-glucosaminidase (EC 3.2.1.30) activity and on hyphal morphological patterns in pellets of Aspergillus niger was studied. It was found that changes in the degree of branching and, to a lesser extent, in the number of bulbous cells were directly related to the activity of the enzyme. Nevertheless, since beta-N-acetyl-D-glucosaminidase is not the only enzyme involved in the lytic potential of the fungus, these findings do not exclude the possibility that other enzymes may be involved. PMID:10398828

  11. Hyphal Branching during Arbuscule Development Requires Reduced Arbuscular Mycorrhiza1.

    PubMed

    Park, Hee-Jin; Floss, Daniela S; Levesque-Tremblay, Veronique; Bravo, Armando; Harrison, Maria J

    2015-12-01

    During arbuscular mycorrhizal symbiosis, arbuscule development in the root cortical cell and simultaneous deposition of the plant periarbuscular membrane generate the interface for symbiotic nutrient exchange. The transcriptional changes that accompany arbuscule development are extensive and well documented. By contrast, the transcriptional regulators that control these programs are largely unknown. Here, we provide a detailed characterization of an insertion allele of Medicago truncatula Reduced Arbuscular Mycorrhiza1 (RAM1), ram1-3, which reveals that RAM1 is not necessary to enable hyphopodium formation or hyphal entry into the root but is essential to support arbuscule branching. In ram1-3, arbuscules consist only of the arbuscule trunk and in some cases, a few initial thick hyphal branches. ram1-3 is also insensitive to phosphate-mediated regulation of the symbiosis. Transcript analysis of ram1-3 and ectopic expression of RAM1 indicate that RAM1 regulates expression of EXO70I and Stunted Arbuscule, two genes whose loss of function impacts arbuscule branching. Furthermore, RAM1 regulates expression of a transcription factor Required for Arbuscule Development (RAD1). RAD1 is also required for arbuscular mycorrhizal symbiosis, and rad1 mutants show reduced colonization. RAM1 itself is induced in colonized root cortical cells, and expression of RAM1 and RAD1 is modulated by DELLAs. Thus, the data suggest that DELLAs regulate arbuscule development through modulation of RAM1 and RAD1 and that the precise transcriptional control essential to place proteins in the periarbuscular membrane is controlled, at least in part, by RAM1.

  12. The AngFus3 Mitogen-Activated Protein Kinase Controls Hyphal Differentiation and Secondary Metabolism in Aspergillus niger.

    PubMed

    Priegnitz, Bert-Ewald; Brandt, Ulrike; Pahirulzaman, Khomaizon A K; Dickschat, Jeroen S; Fleißner, André

    2015-06-01

    Adaptation to a changing environment is essential for the survival and propagation of sessile organisms, such as plants or fungi. Filamentous fungi commonly respond to a worsening of their growth conditions by differentiation of asexually or sexually produced spores. The formation of these specialized cell types is, however, also triggered as part of the general life cycle by hyphal age or density. Spores typically serve for dispersal and, therefore, translocation but can also act as resting states to endure times of scarcity. Eukaryotic differentiation in response to environmental and self-derived signals is commonly mediated by three-tiered mitogen-activated protein (MAP) kinase signaling cascades. Here, we report that the MAP kinase Fus3 of the black mold Aspergillus niger (AngFus3) and its upstream kinase AngSte7 control vegetative spore formation and secondary metabolism. Mutants lacking these kinases are defective in conidium induction in response to hyphal density but are fully competent in starvation-induced sporulation, indicating that conidiation in A. niger is triggered by various independent signals. In addition, the mutants exhibit an altered profile of volatile metabolites and secrete dark pigments into the growth medium, suggesting a dysregulation of the secondary metabolism. By assigning the AngFus3 MAP kinase pathway to the transduction of a potentially self-derived trigger, this work contributes to the unraveling of the intricate signaling networks controlling fungal differentiation. Moreover, our data further support earlier observations that differentiation and secondary metabolism are tightly linked in filamentous fungi.

  13. HOY1, a homeo gene required for hyphal formation in Yarrowia lipolytica.

    PubMed Central

    Torres-Guzmán, J C; Domínguez, A

    1997-01-01

    The dimorphic fungus Yarrowia lipolytica grows to form hyphae either in rich media or in media with GlcNAc as a carbon source. A visual screening, called FIL (filamentation minus), for Y. lipolytica yeast growth mutants has been developed. The FIL screen was used to identify three Y. lipolytica genes that abolish hypha formation in all media assayed. Y. lipolytica HOY1, a gene whose deletion prevents the yeast-hypha transition both in liquid and solid media, was characterized. HOY1 is predicted to encode a 509-amino-acid protein with a homeodomain homologous to that found in the chicken Hox4.8 gene. Analysis of the protein predicts a nuclear location. These observations suggest that Hoy1p may function as a transcriptional regulatory protein. In disrupted strains, reintroduction of HOY1 restored the capacity for hypha formation. Northern blot hybridization revealed the HOY1 transcript to be approximately 1.6 kb. Expression of this gene was detected when Y. lipolytica grew as a budding yeast, but an increase in its expression was observed by 1 h after cells had been induced to form hyphae. The possible functions of HOY1 in hyphal growth and the uses of the FIL screen to identify morphogenetic regulatory genes from heterologous organisms are discussed. PMID:9343389

  14. Geminivirus-Mediated Delivery of Florigen Promotes Determinate Growth in Aerial Organs and Uncouples Flowering from Photoperiod in Cotton

    PubMed Central

    McGarry, Roisin C.; Ayre, Brian G.

    2012-01-01

    Background Plant architecture and the timing and distribution of reproductive structures are fundamental agronomic traits shaped by patterns of determinate and indeterminate growth. Florigen, encoded by FLOWERING LOCUS T (FT) in Arabidopsis and SINGLE FLOWER TRUSS (SFT) in tomato, acts as a general growth hormone, advancing determinate growth. Domestication of upland cotton (Gossypium hirsutum) converted it from a lanky photoperiodic perennial to a highly inbred, compact day-neutral plant that is managed as an annual row-crop. This dramatic change in plant architecture provides a unique opportunity to analyze the transition from perennial to annual growth. Methodology/Principal Findings To explore these architectural changes, we addressed the role of day-length upon flowering in an ancestral, perennial accession and in a domesticated variety of cotton. Using a disarmed Cotton leaf crumple virus (CLCrV) as a transient expression system, we delivered FT to both cotton accessions. Ectopic expression of FT in ancestral cotton mimicked the effects of day-length, promoting photoperiod-independent flowering, precocious determinate architecture, and lanceolate leaf shape. Domesticated cotton infected with FT demonstrated more synchronized fruiting and enhanced “annualization”. Transient expression of FT also facilitated simple crosses between wild photoperiodic and domesticated day-neutral accessions, effectively demonstrating a mechanism to increase genetic diversity among cultivated lines of cotton. Virus was not detected in the F1 progeny, indicating that crosses made by this approach do not harbor recombinant DNA molecules. Conclusions These findings extend our understanding of FT as a general growth hormone that regulates shoot architecture by advancing organ-specific and age-related determinate growth. Judicious manipulation of FT could benefit cotton architecture to improve crop management. PMID:22615805

  15. Effect of crop growth and canopy filtration on the dynamics of plant disease epidemics spread by aerially dispersed spores.

    PubMed

    Ferrandino, F J

    2008-05-01

    Most mathematical models of plant disease epidemics ignore the growth and phenology of the host crop. Unfortunately, reports of disease development are often not accompanied by a simultaneous and commensurate evaluation of crop development. However, the time scale for increases in the leaf area of field crops is comparable to the time scale of epidemics. This simultaneous development of host and pathogen has many ramifications on the resulting plant disease epidemic. First, there is a simple dilution effect resulting from the introduction of new healthy leaf area with time. Often, measurements of disease levels are made pro rata (per unit of host leaf area or total root length or mass). Thus, host growth will reduce the apparent infection rate. A second, related effect, has to do with the so-called "correction factor," which accounts for inoculum falling on already infected tissue. This factor accounts for multiple infection and is given by the fraction of the host tissue that is susceptible to disease. As an epidemic develops, less and less tissue is open to infection and the initial exponential growth slows. Crop growth delays the impact of this limiting effect and, therefore, tends to increase the rate of disease progress. A third and often neglected effect arises when an increase in the density of susceptible host tissue results in a corresponding increase in the basic reproduction ratio, R(0), defined as the ratio of the total number of daughter lesions produced to the number of original mother lesions. This occurs when the transport efficiency of inoculum from infected to susceptible host is strongly dependent on the spatial density of plant tissue. Thus, crop growth may have a major impact on the development of plant disease epidemics occurring during the vegetative phase of crop growth. The effects that these crop growth-related factors have on plant disease epidemics spread by airborne spores are evaluated using mathematical models and their importance is

  16. Vegetative hyphal fusion and subsequent nuclear behavior in Epichloë grass endophytes.

    PubMed

    Shoji, Jun-Ya; Charlton, Nikki D; Yi, Mihwa; Young, Carolyn A; Craven, Kelly D

    2015-01-01

    Epichloë species (including the former genus Neotyphodium) are fungal symbionts of many agronomically important forage grasses, and provide their grass hosts with protection from a wide range of biotic and abiotic stresses. Epichloë species include many interspecific hybrids with allodiploid-like genomes, which may provide the potential for combined traits or recombination to generate new traits. Though circumstantial evidence suggests that such interspecific hybrids might have arisen from nuclear fusion events following vegetative hyphal fusion between different Epichloë strains, this hypothesis has not been addressed empirically. Here, we investigated vegetative hyphal fusion and subsequent nuclear behavior in Epichloë species. A majority of Epichloë strains, especially those having a sexual stage, underwent self vegetative hyphal fusion. Vegetative fusion also occurred between two hyphae from different Epichloë strains. Though Epichloë spp. are uninucleate fungi, hyphal fusion resulted in two nuclei stably sharing the same cytoplasm, which might ultimately lead to nuclear fusion. In addition, protoplast fusion experiments gave rise to uninucleate putative hybrids, which apparently had two markers, one from each parent within the same nucleus. These results are consistent with the notion that interspecific hybrids arise from vegetative hyphal fusion. However, we also discuss additional factors, such as post-hybridization selection, that may be important to explain the recognized prevalence of hybrids in Epichloë species.

  17. The AngFus3 Mitogen-Activated Protein Kinase Controls Hyphal Differentiation and Secondary Metabolism in Aspergillus niger

    PubMed Central

    Priegnitz, Bert-Ewald; Brandt, Ulrike; Pahirulzaman, Khomaizon A. K.; Dickschat, Jeroen S.

    2015-01-01

    Adaptation to a changing environment is essential for the survival and propagation of sessile organisms, such as plants or fungi. Filamentous fungi commonly respond to a worsening of their growth conditions by differentiation of asexually or sexually produced spores. The formation of these specialized cell types is, however, also triggered as part of the general life cycle by hyphal age or density. Spores typically serve for dispersal and, therefore, translocation but can also act as resting states to endure times of scarcity. Eukaryotic differentiation in response to environmental and self-derived signals is commonly mediated by three-tiered mitogen-activated protein (MAP) kinase signaling cascades. Here, we report that the MAP kinase Fus3 of the black mold Aspergillus niger (AngFus3) and its upstream kinase AngSte7 control vegetative spore formation and secondary metabolism. Mutants lacking these kinases are defective in conidium induction in response to hyphal density but are fully competent in starvation-induced sporulation, indicating that conidiation in A. niger is triggered by various independent signals. In addition, the mutants exhibit an altered profile of volatile metabolites and secrete dark pigments into the growth medium, suggesting a dysregulation of the secondary metabolism. By assigning the AngFus3 MAP kinase pathway to the transduction of a potentially self-derived trigger, this work contributes to the unraveling of the intricate signaling networks controlling fungal differentiation. Moreover, our data further support earlier observations that differentiation and secondary metabolism are tightly linked in filamentous fungi. PMID:25888553

  18. Aerial shaking performance of wet Anna's hummingbirds.

    PubMed

    Ortega-Jimenez, Victor Manuel; Dudley, Robert

    2012-05-01

    External wetting poses problems of immediate heat loss and long-term pathogen growth for vertebrates. Beyond these risks, the locomotor ability of smaller animals, and particularly of fliers, may be impaired by water adhering to the body. Here, we report on the remarkable ability of hummingbirds to perform rapid shakes in order to expel water from their plumage even while in flight. Kinematic performance of aerial versus non-aerial shakes (i.e. those performed while perching) was compared. Oscillation frequencies of the head, body and tail were lower in aerial shakes. Tangential speeds and accelerations of the trunk and tail were roughly similar in aerial and non-aerial shakes, but values for head motions while perching were twice as high when compared with aerial shakes [corrected] . Azimuthal angular amplitudes for both aerial and non-aerial shakes reached values greater than 180° for the head, greater than 45° for the body trunk and slightly greater than 90° for the tail and wings. Using a feather on an oscillating disc to mimic shaking motions, we found that bending increased average speeds by up to 36 per cent and accelerations of the feather tip up to fourfold relative to a hypothetical rigid feather. Feather flexibility may help to enhance shedding of water and reduce body oscillations during shaking.

  19. Aerial radiation surveys

    SciTech Connect

    Jobst, J.

    1980-01-01

    A recent aerial radiation survey of the surroundings of the Vitro mill in Salt Lake City shows that uranium mill tailings have been removed to many locations outside their original boundary. To date, 52 remote sites have been discovered within a 100 square kilometer aerial survey perimeter surrounding the mill; 9 of these were discovered with the recent aerial survey map. Five additional sites, also discovered by aerial survey, contained uranium ore, milling equipment, or radioactive slag. Because of the success of this survey, plans are being made to extend the aerial survey program to other parts of the Salt Lake valley where diversions of Vitro tailings are also known to exist.

  20. Ultrastructural localization of anionic sites on the surface of yeast, hyphal and germ-tube forming cells of Candida albicans.

    PubMed

    Horisberger, M; Clerc, M F

    1988-08-01

    The cell wall of Candida albicans contains chitin, beta-glucans and phosphorylated mannoproteins, and possesses a fuzzy coat which is thought to play a role in pathogenicity, phagocytosis, and adherence of this dimorphic yeast. Using scanning electron microscopy and the gold method, mannoproteins were detected on the whole surface of blastoconidia including the bud scars, but chitin was absent even after alpha-mannosidase treatment of the cells. The presence of surface beta-(1----6)glucan (but not beta(1----3)glucan) was observed only after extensive alpha-mannosidase and alkaline phosphatase treatments of blastoconidia. Using transmission and scanning electron microscopy, the locations of anionic sites were revealed by polycationic colloidal gold-chitosan complexes on the surface of blastoconidia, germ tubes and hyphae. Anionic sites were dispersed evenly over the surface of blastoconidia bearing bud scars. Depending upon the growth conditions, anionic sites could be detected on emerging buds and young cells. However, bud scars were always free of marking. When germ-tube formation was induced, anionic sites were present at different densities on all cell surfaces, the highest density being observed on cells with bud scars. Anionic sites were detected at a remarkably high density on all hyphal surfaces. An apical concentration of anionic sites was observed on germ tubes and hyphae. The distribution of anionic sites was not modified by endoglucosaminidase treatment of blastoconidia, germ tubes and hyphae. The anionic sites were associated with the fuzzy coat. As the hyphal form is regarded as possessing the greatest invasiveness, it is suggested that anionic sites play an important role in establishing tissue colonization by this human pathogen. PMID:3053174

  1. Aerial Image Systems

    NASA Astrophysics Data System (ADS)

    Clapp, Robert E.

    1987-09-01

    Aerial images produce the best stereoscopic images of the viewed world. Despite the fact that every optic in existence produces an aerial image, few persons are aware of their existence and possible uses. Constant reference to the eye and other optical systems have produced a psychosis of design that only considers "focal planes" in the design and analysis of optical systems. All objects in the field of view of the optical device are imaged by the device as an aerial image. Use of aerial images in vision and visual display systems can provide a true stereoscopic representation of the viewed world. This paper discusses aerial image systems - their applications and designs and presents designs and design concepts that utilize aerial images to obtain superior visual displays, particularly with application to visual simulation.

  2. Does percent root length colonization and soil hyphal length reflect the extent of colonization for all AMF?

    PubMed

    Hart, Miranda M; Reader, Richard J

    2002-12-01

    Percent root length colonization may not be an appropriate measure of root colonization by arbuscular mycorrhizal fungi (AMF) in all cases. We suggest that AMF will differ in how well percent root length colonization measures the amount of AMF colonization in the root due to differences among AMF in hyphal structure and hyphal aggregation. Although soil hyphal length accounts for hyphal density, we suggest that it does not consider differences in hyphal structure in measurements of external colonization and thus might also misrepresent the true amount of AMF in the soil. To test these suggestions, we measured and compared percent root length colonization and soil hyphal length with root ergosterol and soil ergosterol, respectively, for 21 different species of AMF from three families in a greenhouse experiment. Percent root length colonization predicted intra-radical colonization best for Glomaceae and Acaulosporaceae isolates, while soil hyphal length best represented soil ergosterol for Gigasporaceae isolates. The results show that conventional methods for estimating AMF colonization are not universal for all AMF. Caution is advised when drawing inferences for different groups of AMF.

  3. 11. Photocopy of aerial photograph (original aerial located in the ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Photocopy of aerial photograph (original aerial located in the U.S. Forest Service, Toiyabe National Forest, Carson District Office). AERIAL VIEW OF THE GENOA PEAK ROAD, SPUR. - Genoa Peak Road, Spur, Glenbrook, Douglas County, NV

  4. Aerial photographic reproductions

    USGS Publications Warehouse

    ,

    1975-01-01

    The National Cartographic Information Center of the U.S. Geological Survey maintains records of aerial photographic coverage of the United States and its Territories, based on reports from other Federal agencies as well as State governmental agencies and commercial companies. From these records, the Center furnishes data to prospective purchasers on available photography and the agency holding the aerial film.

  5. Loss of NAD(P)-reducing power and glutathione disulfide excretion at the start of induction of aerial growth in Neurospora crassa.

    PubMed

    Toledo, I; Noronha-Dutra, A A; Hansberg, W

    1991-05-01

    When exponentially growing hyphae of Neurospora crassa in aerated liquid cultures are filtered and the resulting mycelial mat is exposed to air, aerial hyphae develop and synchronous conidiation is obtained. The hyphae in direct contact with air adhere to each other within minutes and form aerial hyphae during the following 12 h; the hyphae which are not in direct contact with air do not adhere to each other and do not form aerial hyphae. Previous data indicated that oxidative stress was generated in the adhering hyphae; proteins and specific enzymes were found to be oxidatively modified and degraded. In this work, we report a dramatic fall in the reduced-to-oxidized ratio of NAD and NADP coenzymes during the first 6 min of exposure to air. This drop did not occur in a mycelial mat exposed to a N2-enriched atmosphere. Adding a carbon source to the mycelial mat did not abolish the loss of NAD(P)-reducing power. After the initial fall, the reducing levels of the coenzymes returned to the starting value in about 30 min. A peak of extracellular glutathione disulfide occurred simultaneously with the loss of NAD(P)-reducing power. The reducing power loss and the excretion of glutathione disulfide are thought to be consequences of a hyperoxidant state; the adhesion of hyphae is thought to be a response to the hyperoxidant state.

  6. Staphylococcus aureus adherence to Candida albicans hyphae is mediated by the hyphal adhesin Als3p

    PubMed Central

    Peters, Brian M.; Ovchinnikova, Ekaterina S.; Krom, Bastiaan P.; Schlecht, Lisa Marie; Zhou, Han; Hoyer, Lois L.; Busscher, Henk J.; van der Mei, Henny C.; Jabra-Rizk, Mary Ann

    2012-01-01

    The bacterium Staphylococcus (St.) aureus and the opportunistic fungus Candida albicans are currently among the leading nosocomial pathogens, often co-infecting critically ill patients, with high morbidity and mortality. Previous investigations have demonstrated preferential adherence of St. aureus to C. albicans hyphae during mixed biofilm growth. In this study, we aimed to characterize the mechanism behind this observed interaction. C. albicans adhesin-deficient mutant strains were screened by microscopy to identify the specific receptor on C. albicans hyphae recognized by St. aureus. Furthermore, an immunoassay was developed to validate and quantify staphylococcal binding to fungal biofilms. The findings from these experiments implicated the C. albicans adhesin agglutinin-like sequence 3 (Als3p) in playing a major role in the adherence process. This association was quantitatively established using atomic force microscopy, in which the adhesion force between single cells of the two species was significantly reduced for a C. albicans mutant strain lacking als3. Confocal microscopy further confirmed these observations, as St. aureus overlaid with a purified recombinant Als3 N-terminal domain fragment (rAls3p) exhibited robust binding. Importantly, a strain of Saccharomyces cerevisiae heterologously expressing Als3p was utilized to further confirm this adhesin as a receptor for St. aureus. Although the parental strain does not bind bacteria, expression of Als3p on the cell surface conferred upon the yeast the ability to strongly bind St. aureus. To elucidate the implications of these in vitro findings in a clinically relevant setting, an ex vivo murine model of co-infection was designed using murine tongue explants. Fluorescent microscopic images revealed extensive hyphal penetration of the epithelium typical of C. albicans mucosal infection. Interestingly, St. aureus bacterial cells were only seen within the epithelial tissue when associated with the invasive

  7. Hyphal heterogeneity in Aspergillus oryzae is the result of dynamic closure of septa by Woronin bodies.

    PubMed

    Bleichrodt, Robert-Jan; van Veluw, G Jerre; Recter, Brand; Maruyama, Jun-Ichi; Kitamoto, Katsuhiko; Wösten, Han A B

    2012-12-01

    Hyphae of higher fungi are compartmentalized by septa. These septa contain a central pore that allows for inter-compartmental and inter-hyphal cytoplasmic streaming. The cytoplasm within the mycelium is therefore considered to be a continuous system. In this study, however, we demonstrate by laser dissection that 40% of the apical septa of exploring hyphae of Aspergillus oryzae are closed. Closure of septa correlated with the presence of a peroxisome-derived organelle, known as Woronin body, near the septal pore. The location of Woronin bodies in the hyphae was dynamic and, as a result, plugging of the septal pore was reversible. Septal plugging was abolished in a ΔAohex1 strain that cannot form Woronin bodies. Notably, hyphal heterogeneity was also affected in the ΔAohex1 strain. Wild-type strains of A. oryzae showed heterogeneous distribution of GFP between neighbouring hyphae at the outer part of the colony when the reporter was expressed from the promoter of the glucoamylase gene glaA or the α-glucuronidase gene aguA. In contrast, GFP fluorescence showed a normal distribution in the case of the ΔAohex1 strain. Taken together, it is concluded that Woronin bodies maintain hyphal heterogeneity in a fungal mycelium by impeding cytoplasmic continuity.

  8. Systemic Staphylococcus aureus infection mediated by Candida albicans hyphal invasion of mucosal tissue

    PubMed Central

    Schlecht, Lisa Marie; Peters, Brian M.; Krom, Bastiaan P.; Freiberg, Jeffrey A.; Hänsch, Gertrud M.; Filler, Scott G.

    2015-01-01

    Candida albicans and Staphylococcus aureus are often co-isolated in cases of biofilm-associated infections. C. albicans can cause systemic disease through morphological switch from the rounded yeast to the invasive hyphal form. Alternatively, systemic S. aureus infections arise from seeding through breaks in host epithelial layers although many patients have no documented portal of entry. We describe a novel strategy by which S. aureus is able to invade host tissue and disseminate via adherence to the invasive hyphal elements of Candida albicans. In vitro and ex vivo findings demonstrate a specific binding of the staphylococci to the candida hyphal elements. The C. albicans cell wall adhesin Als3p binds to multiple staphylococcal adhesins. Furthermore, Als3p is required for C. albicans to transport S. aureus into the tissue and cause a disseminated infection in an oral co-colonization model. These findings suggest that C. albicans can facilitate the invasion of S. aureus across mucosal barriers, leading to systemic infection in co-colonized patients. PMID:25332378

  9. Aerial Photography Summary Record System

    USGS Publications Warehouse

    ,

    1998-01-01

    The Aerial Photography Summary Record System (APSRS) describes aerial photography projects that meet specified criteria over a given geographic area of the United States and its territories. Aerial photographs are an important tool in cartography and a number of other professions. Land use planners, real estate developers, lawyers, environmental specialists, and many other professionals rely on detailed and timely aerial photographs. Until 1975, there was no systematic approach to locate an aerial photograph, or series of photographs, quickly and easily. In that year, the U.S. Geological Survey (USGS) inaugurated the APSRS, which has become a standard reference for users of aerial photographs.

  10. Inhibitory Effect of Sophorolipid on Candida albicans Biofilm Formation and Hyphal Growth

    PubMed Central

    Haque, Farazul; Alfatah, Md.; Ganesan, K.; Bhattacharyya, Mani Shankar

    2016-01-01

    Candida albicans causes superficial and life-threatening systemic infections. These are difficult to treat often due to drug resistance, particularly because C. albicans biofilms are inherently resistant to most antifungals. Sophorolipid (SL), a glycolipid biosurfactant, has been shown to have antimicrobial and anticancer properties. In this study, we investigated the effect of SL on C. albicans biofilm formation and preformed biofilms. SL was found to inhibit C. albicans biofilm formation as well as reduce the viability of preformed biofilms. Moreover, SL, when used along with amphotericin B (AmB) or fluconazole (FLZ), was found to act synergistically against biofilm formation and preformed biofilms. Effect of SL on C. albicans biofilm formation was further visualized by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM), which revealed absence of hyphae, typical biofilm architecture and alteration in the morphology of biofilm cells. We also found that SL downregulates the expression of hypha specific genes HWP1, ALS1, ALS3, ECE1 and SAP4, which possibly explains the inhibitory effect of SL on hyphae and biofilm formation. PMID:27030404

  11. Quantitative analysis of concentration gradient and ionic currents associated with hyphal tip growth in fungi

    NASA Astrophysics Data System (ADS)

    Limozin, L.; Denet, B.

    2000-09-01

    It has been shown previously that the nutrient gradient generated by a tip growing elongated cell induces an ionic current entering the cell tip and looping back in the extracellular medium [L. Limozin, B. Denet and P. Pelcé, Phys. Rev. Lett. 78, 4881 (1997)]. We apply this mechanism to the case of hyphae of fungi, using realistic cell geometries, symport kinetics, proton pump permeabilities, and buffer concentrations. We show that this mechanism contributes to a noticeable part of the external current intensity, related inner electrical field and pH gradient, in agreement with experimental measurements. This provides a good example in biological cells of interaction between shape and field, a common property of growing nonliving systems, such as crystalline dendrites or electrodeposition.

  12. Significance of hyphae formation in virulence of Candida tropicalis and transcriptomic analysis of hyphal cells.

    PubMed

    Jiang, Cen; Li, Zhen; Zhang, Lihua; Tian, Yuan; Dong, Danfeng; Peng, Yibing

    2016-11-01

    Recently, the proportion of Candida tropicalis in clinical isolates has significantly increased. Some C. tropicalis strains colonize the skin or mucosal surfaces as commensals; others trigger invasive infection. To date, the pathogenicity of C. tropicalis has not been thoroughly researched. This study reports several virulence factors, including biofilm and hyphae formation, proteinase, phospholipase, lipase and hemolytic activity, in 52 clinical isolates of C. tropicalis collected from five hospitals in four provinces of China. Some C. tropicalis tended to produce more hyphae than others in the same circumstance. Six C. tropicalis strains with different morphologies were injected into mice via the tail vein, and the survival proportions and fungal burdens of the strains were evaluated. Hyphal production by C. tropicalis was associated with stronger virulence. RNA sequencing revealed that C. tropicalis with more hyphae up-regulated several genes involved in morphological differentiation and oxidative response, including IF2, Atx1, and Sod2. It appears that hyphal formation plays a vital role in the pathogenicity of C. tropicalis, and interacts with the oxidative stress response to strengthen the organism's virulence. PMID:27664724

  13. A developmental stage of hyphal cells shows riboflavin overproduction instead of sporulation in Ashbya gossypii.

    PubMed

    Nieland, Susanne; Stahmann, K-Peter

    2013-12-01

    The hemiascomycete Ashbya gossypii develops a mycelium. Nutritional stress leads to its differentiation into sporangia. These generate spores. In parallel, the yellow pigment riboflavin is produced. Intracellularly accumulated riboflavin, made visible as a bright green fluorescence, was observed in only 60% of the hyphal cells. For the remaining 40%, it was unclear whether these cells simply export riboflavin or its biosynthesis remains down-regulated in contrast to the accumulating cells. The approach followed in this work was to convert the hyphae into protoplasts by enzymatic degradation of the cell wall. Afterwards, the protoplasts were sorted by fluorescence-activated cell sorting on the basis of riboflavin accumulation. When a reporter strain expressing lacZ under the control of the most important riboflavin biosynthesis promoter, RIB3, was used, green protoplasts were found to have more than tenfold greater reporter activity than hyaline protoplasts. This was true on the basis of total protein as well as on the basis of hexokinase specific activity, a marker for constitutive expression. These results allow the conclusion that hyphal cells of A. gossypii differ in phenotype regarding riboflavin overproduction and accumulation. PMID:24092010

  14. Waikialoid A Suppresses Hyphal Morphogenesis and Inhibits Biofilm Development in Pathogenic Candida albicans

    PubMed Central

    Wang, Xiaoru; You, Jianlan; King, Jarrod B.; Powell, Douglas R.; Cichewicz, Robert H.

    2012-01-01

    A chemically prolific strain of Aspergillus was isolated from a soil sample collected near Waikiki Beach, Honolulu, Hawaii. The fungus produced several secondary metabolites that were purified and placed in our natural products library, which was later screened for substances capable of inhibiting biofilm formation by Candida albicans. It was determined that one of the secondary metabolites from the Hawaiian fungal isolate, a new complex prenylated indole alkaloid named waikialoid A (1), inhibited biofilm formation with an IC50 value of 1.4 μM. Another structurally unrelated, presumably polyketide metabolite, waikialide A (15), also inhibited C. albicans biofilm formation, but was much less potent (IC50 value of 32.4 μM). Microscopy studies revealed that compound 1 also inhibited C. albicans hyphal morphogenesis. While metabolite 1 appears ineffective at disrupting preformed biofilms, the accumulated data indicate that the new compound may exert its activity against C. ablicans during the early stages of surface colonization involving cell adherence, hyphal development, and/or biofilm assembly. Unlike some other stephacidin/notoamide compounds, metabolite 1 was not cytotoxic to fungi or human cells (up to 200 μM), which makes this an intriguing model compound for studying the adjunctive use of biofilm inhibitors in combination with standard antifungal antibiotics. PMID:22400916

  15. Waikialoid A suppresses hyphal morphogenesis and inhibits biofilm development in pathogenic Candida albicans.

    PubMed

    Wang, Xiaoru; You, Jianlan; King, Jarrod B; Powell, Douglas R; Cichewicz, Robert H

    2012-04-27

    A chemically prolific strain of Aspergillus was isolated from a soil sample collected near Waikiki Beach, Honolulu, Hawaii. The fungus produced several secondary metabolites, which were purified and placed in our natural products library and were later screened for substances capable of inhibiting biofilm formation by Candida albicans. It was determined that one of the secondary metabolites from the Hawaiian fungal isolate, a new complex prenylated indole alkaloid named waikialoid A (1), inhibited biofilm formation with an IC(50) value of 1.4 μM. Another structurally unrelated, presumably polyketide metabolite, waikialide A (15), also inhibited C. albicans biofilm formation, but was much less potent (IC(50) value of 32.4 μM). Microscopy studies revealed that compound 1 also inhibited C. albicans hyphal morphogenesis. While metabolite 1 appears ineffective at disrupting preformed biofilms, the accumulated data indicate that the new compound may exert its activity against C. albicans during the early stages of surface colonization involving cell adherence, hyphal development, and/or biofilm assembly. Unlike some other stephacidin/notoamide compounds, metabolite 1 was not cytotoxic to fungi or human cells (up to 200 μM), which makes this an intriguing model compound for studying the adjunctive use of biofilm inhibitors in combination with standard antifungal antibiotics. PMID:22400916

  16. Aerial Explorers and Robotic Ecosystems

    NASA Technical Reports Server (NTRS)

    Young, Larry A.; Pisanich, Greg

    2004-01-01

    A unique bio-inspired approach to autonomous aerial vehicle, a.k.a. aerial explorer technology is discussed. The work is focused on defining and studying aerial explorer mission concepts, both as an individual robotic system and as a member of a small robotic "ecosystem." Members of this robotic ecosystem include the aerial explorer, air-deployed sensors and robotic symbiotes, and other assets such as rovers, landers, and orbiters.

  17. Aerial Perspective Artistry

    ERIC Educational Resources Information Center

    Wolfe, Linda

    2010-01-01

    This article presents a lesson centering on aerial perspective artistry of students and offers suggestions on how art teachers should carry this project out. This project serves to develop students' visual perception by studying reproductions by famous artists. This lesson allows one to imagine being lured into a landscape capable of captivating…

  18. Aerial of the VAB

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Even in this aerial view at KSC, the Vehicle Assembly Building is imposing. In front of it is the Launch Control Center. In the background is the Rotation/Processing Facility, next to the Banana Creek. In the foreground is the Saturn Causeway that leads to Launch Pads 39A and 39B.

  19. Aerial photographic reproductions

    USGS Publications Warehouse

    U.S. Geological Survey

    1971-01-01

    Geological Survey vertical aerial photography is obtained primarily for topographic and geologic mapping. Reproductions from this photography are usually satisfactory for general use. Because reproductions are not stocked, but are custom processed for each order, they cannot be returned for credit or refund.

  20. Differential activity of Striga hermonthica seed germination stimulants and Gigaspora rosea hyphal branching factors in rice and their contribution to underground communication.

    PubMed

    Cardoso, Catarina; Charnikhova, Tatsiana; Jamil, Muhammad; Delaux, Pierre-Marc; Verstappen, Francel; Amini, Maryam; Lauressergues, Dominique; Ruyter-Spira, Carolien; Bouwmeester, Harro

    2014-01-01

    Strigolactones (SLs) trigger germination of parasitic plant seeds and hyphal branching of symbiotic arbuscular mycorrhizal (AM) fungi. There is extensive structural variation in SLs and plants usually produce blends of different SLs. The structural variation among natural SLs has been shown to impact their biological activity as hyphal branching and parasitic plant seed germination stimulants. In this study, rice root exudates were fractioned by HPLC. The resulting fractions were analyzed by MRM-LC-MS to investigate the presence of SLs and tested using bioassays to assess their Striga hermonthica seed germination and Gigaspora rosea hyphal branching stimulatory activities. A substantial number of active fractions were revealed often with very different effect on seed germination and hyphal branching. Fractions containing (-)-orobanchol and ent-2'-epi-5-deoxystrigol contributed little to the induction of S. hermonthica seed germination but strongly stimulated AM fungal hyphal branching. Three SLs in one fraction, putative methoxy-5-deoxystrigol isomers, had moderate seed germination and hyphal branching inducing activity. Two fractions contained strong germination stimulants but displayed only modest hyphal branching activity. We provide evidence that these stimulants are likely SLs although no SL-representative masses could be detected using MRM-LC-MS. Our results show that seed germination and hyphal branching are induced to very different extents by the various SLs (or other stimulants) present in rice root exudates. We propose that the development of rice varieties with different SL composition is a promising strategy to reduce parasitic plant infestation while maintaining symbiosis with AM fungi.

  1. The hyphal wall of Mucor mucedo. 2. Hexosamine-containing polymers.

    PubMed

    Datema, R; Wessels, J G; van den Ende, H

    1977-11-01

    Nitrous acid, which specifically depolymerises polymers containing hexosamines with a primary amino group, was used to analyse the hexosamine-containing polymers in the hyphal wall of Mucor mucedo. N-Acetylglucosamine was found to occur in three polymeric fractions. One fraction which was solubilised by HNO2 treatment contained-N-acetylglucosamine interspersed with glucosamine; no homopolymer of glucosamine (chitosan) was detected. Another fraction became HNO2-soluble after treatment with pronase or alkali; this points to the occurrence of a heteropolymer containing N-acetylglucosamine and glucosamine in which some of the glucosamine residues are linked to peptides via their amino groups. The residue remaaining after pronase and HNO* treatment appeared to consist of a homopolymer of N-acetylglucosamine (chitin).

  2. Hyphal Branching during Arbuscule Development Requires Reduced Arbuscular Mycorrhiza11[OPEN

    PubMed Central

    Park, Hee-Jin; Floss, Daniela S.; Levesque-Tremblay, Veronique; Bravo, Armando

    2015-01-01

    During arbuscular mycorrhizal symbiosis, arbuscule development in the root cortical cell and simultaneous deposition of the plant periarbuscular membrane generate the interface for symbiotic nutrient exchange. The transcriptional changes that accompany arbuscule development are extensive and well documented. By contrast, the transcriptional regulators that control these programs are largely unknown. Here, we provide a detailed characterization of an insertion allele of Medicago truncatula Reduced Arbuscular Mycorrhiza1 (RAM1), ram1-3, which reveals that RAM1 is not necessary to enable hyphopodium formation or hyphal entry into the root but is essential to support arbuscule branching. In ram1-3, arbuscules consist only of the arbuscule trunk and in some cases, a few initial thick hyphal branches. ram1-3 is also insensitive to phosphate-mediated regulation of the symbiosis. Transcript analysis of ram1-3 and ectopic expression of RAM1 indicate that RAM1 regulates expression of EXO70I and Stunted Arbuscule, two genes whose loss of function impacts arbuscule branching. Furthermore, RAM1 regulates expression of a transcription factor Required for Arbuscule Development (RAD1). RAD1 is also required for arbuscular mycorrhizal symbiosis, and rad1 mutants show reduced colonization. RAM1 itself is induced in colonized root cortical cells, and expression of RAM1 and RAD1 is modulated by DELLAs. Thus, the data suggest that DELLAs regulate arbuscule development through modulation of RAM1 and RAD1 and that the precise transcriptional control essential to place proteins in the periarbuscular membrane is controlled, at least in part, by RAM1. PMID:26511916

  3. [Demonstration of β-1,2 mannan structures expressed on the cell wall of Candida albicans yeast form but not on the hyphal form by using monoclonal antibodies].

    PubMed

    Aydın, Cevahir; Ataoğlu, Haluk

    2015-01-01

    Candida albicans is a polymorphic fungus that may be observed as both commensal and opportunistic pathogen in humans. As one of the major components of Candida cell wall structure, mannan plays an important role in the fungus-host cell interaction and in virulence. The ability to switch from yeast to hypha form of microorganism is crutial in the development of C.albicans infections. Hyphal form has different antigenic properties compared to yeast form and structural changes occur in the yeast cell wall during transition from yeast to hypha form. Although there are several factors associated with this transition process, sufficient information is not available. The aim of this study was to investigate the change of configuration in mannan structure found in C.albicans cell wall by using monoclonal antibodies. C.albicans (NIHA 207) serotype A strains were used as test strains throughout the study, together with Salmonella choleraesuis 211 and Salmonella infantis as controls with similar cell wall structures to that of C.albicans. Cultures were maintained on YPD-agar medium by incubating at 28°C for yeast forms, and on YPD-broth medium in a shaking incubator at 37°C for 3-4 hours for the growth of hyphal forms. Cells were harvested in the exponential phase, and after being washed, the mannan content from C.albicans were extracted from pellet by heating in 20 mM sodium citrate buffer for 90 minutes at 125°C. Hybridoma technique was used for the production of monoclonal antibodies. After immunizing the Balb/C mice with antigen, the splenocytes were harvested and fusion was performed between spleen cells and F0 myeloma cells. The clones grown in HAT medium were screened for the presence of antibody producing hybrid cells by ELISA method. The antibody isotypes were determined by using a commercial kit (Pierce Biotechnology, ABD). The culture supernatants which contained monoclonal antibodies were collected and purified according to the ammonium sulphate method

  4. AERIAL RADIOLOGICAL SURVEYS

    SciTech Connect

    Proctor, A.E.

    1997-06-09

    Measuring terrestrial gamma radiation from airborne platforms has proved to be a useful method for characterizing radiation levels over large areas. Over 300 aerial radiological surveys have been carried out over the past 25 years including U.S. Department of Energy (DOE) sites, commercial nuclear power plants, Formerly Utilized Sites Remedial Action Program/Uranium Mine Tailing Remedial Action Program (FUSRAP/UMTRAP) sites, nuclear weapons test sites, contaminated industrial areas, and nuclear accident sites. This paper describes the aerial measurement technology currently in use by the Remote Sensing Laboratory (RSL) for routine environmental surveys and emergency response activities. Equipment, data-collection and -analysis methods, and examples of survey results are described.

  5. Novel Structural Features in Candida albicans Hyphal Glucan Provide a Basis for Differential Innate Immune Recognition of Hyphae Versus Yeast*

    PubMed Central

    Lowman, Douglas W.; Greene, Rachel R.; Bearden, Daniel W.; Kruppa, Michael D.; Pottier, Max; Monteiro, Mario A.; Soldatov, Dmitriy V.; Ensley, Harry E.; Cheng, Shih-Chin; Netea, Mihai G.; Williams, David L.

    2014-01-01

    The innate immune system differentially recognizes Candida albicans yeast and hyphae. It is not clear how the innate immune system effectively discriminates between yeast and hyphal forms of C. albicans. Glucans are major components of the fungal cell wall and key fungal pathogen-associated molecular patterns. C. albicans yeast glucan has been characterized; however, little is known about glucan structure in C. albicans hyphae. Using an extraction procedure that minimizes degradation of the native structure, we extracted glucans from C. albicans hyphal cell walls. 1H NMR data analysis revealed that, when compared with reference (1→3,1→6) β-linked glucans and C. albicans yeast glucan, hyphal glucan has a unique cyclical or “closed chain” structure that is not found in yeast glucan. GC/MS analyses showed a high abundance of 3- and 6-linked glucose units when compared with yeast β-glucan. In addition to the expected (1→3), (1→6), and 3,6 linkages, we also identified a 2,3 linkage that has not been reported previously in C. albicans. Hyphal glucan induced robust immune responses in human peripheral blood mononuclear cells and macrophages via a Dectin-1-dependent mechanism. In contrast, C. albicans yeast glucan was a much less potent stimulus. We also demonstrated the capacity of C. albicans hyphal glucan, but not yeast glucan, to induce IL-1β processing and secretion. This finding provides important evidence for understanding the immune discrimination between colonization and invasion at the mucosal level. When taken together, these data provide a structural basis for differential innate immune recognition of C. albicans yeast versus hyphae. PMID:24344127

  6. Regulation of nitrogen uptake and assimilation: Effects of nitrogen source, root-zone pH, and aerial CO2 concentration on growth and productivity of soybeans

    NASA Technical Reports Server (NTRS)

    Raper, C. D.; Tolley-Henry, L.

    1989-01-01

    An important feature of controlled-environment crop production systems such as those to be used for life support of crews during space exploration is the efficient utilization of nitrogen supplies. Making decisions about the best sources of these supplies requires research into the relationship between nitrogen source and the physiological processes which regulate vegetative and reproductive plant growth. Work done in four areas within this research objective is reported: (1) experiments on the effects of root-zone pH on preferential utilization of NO3(-) versus NH4(+) nitrogen; (2) investigation of processes at the whole-plant level that regulate nitrogen uptake; (3) studies of the effects of atmospheric CO2 and NO3(-) supply on the growth of soybeans; and (4) examination of the role of NO3(-) uptake in enhancement of root respiration.

  7. Regulation of nitrogen uptake and assimilation: Effects of nitrogen source and root-zone and aerial environment on growth and productivity of soybean

    NASA Technical Reports Server (NTRS)

    Raper, C. David, Jr.

    1994-01-01

    The interdependence of root and shoot growth produces a functional equilibrium as described in quantitative terms by numerous authors. It was noted that bean seedlings grown in a constant environment tended to have a constant distribution pattern of dry matter between roots and leaves characteristic of the set of environmental conditions. Disturbing equilibrium resulted in a change in relative growth of roots and leaves until the original ratio was restored. To define a physiological basis for regulation of nitrogen uptake within the balance between root and shoot activities, the authors combined a partioning scheme and a utilization priority assumption in which: (1) all carbon enters the plant through photosynthesis in leaves and all nitrogen enters the plant through active uptake by roots, (2) nitrogen uptake by roots and secretion into the xylem for transport to the shoots are active processes, (3) availability of exogenous nitrogen determines concentration of soluble carbohydrates within the roots, (4) leaves are a source and a sink for carbohydrates, and (5) the requirement for nitrogen by leaf growth is proportionally greater during initiation and early expansion than during later expansion.

  8. Aerial Video Imaging

    NASA Technical Reports Server (NTRS)

    1991-01-01

    When Michael Henry wanted to start an aerial video service, he turned to Johnson Space Center for assistance. Two NASA engineers - one had designed and developed TV systems in Apollo, Skylab, Apollo- Soyuz and Space Shuttle programs - designed a wing-mounted fiberglass camera pod. Camera head and angles are adjustable, and the pod is shaped to reduce vibration. The controls are located so a solo pilot can operate the system. A microprocessor displays latitude, longitude, and bearing, and a GPS receiver provides position data for possible legal references. The service has been successfully utilized by railroads, oil companies, real estate companies, etc.

  9. Comparative proteomic analysis reveals intracellular targets for bacillomycin L to induce Rhizoctonia solani Kühn hyphal cell death.

    PubMed

    Zhang, Bao; Qin, Yuxuan; Han, Yuzhu; Dong, Chunjuan; Li, Pinglan; Shang, Qingmao

    2016-09-01

    Bacillomycin L, a natural iturinic lipopeptide produced by Bacillus amyloliquefaciens, is characterized by strong antifungal activity against a variety of agronomically important filamentous fungi including Rhizoctonia solani Kühn. To further understand its antifungal actions, proteomes were comparatively studied within R. solani hyphal cells treated with or without bacillomycin L. The results show that 39 proteins were alternatively expressed within cells in response to this lipopeptide, which are involved in stress response, carbohydrate, amino acid and nucleotide metabolism, cellular component organization, calcium homeostasis, protein degradation, RNA processing, gene transcription, and others, suggesting that, in addition to inducing cell membrane permeabilization, iturin exhibits antibiotic activities by targeting intracellular molecules. Based on these results, a model of action of bacillomycin L against R. solani hyphal cells was proposed. Our study provides new insight into the antibiotic mechanisms of iturins.

  10. Comparative proteomic analysis reveals intracellular targets for bacillomycin L to induce Rhizoctonia solani Kühn hyphal cell death.

    PubMed

    Zhang, Bao; Qin, Yuxuan; Han, Yuzhu; Dong, Chunjuan; Li, Pinglan; Shang, Qingmao

    2016-09-01

    Bacillomycin L, a natural iturinic lipopeptide produced by Bacillus amyloliquefaciens, is characterized by strong antifungal activity against a variety of agronomically important filamentous fungi including Rhizoctonia solani Kühn. To further understand its antifungal actions, proteomes were comparatively studied within R. solani hyphal cells treated with or without bacillomycin L. The results show that 39 proteins were alternatively expressed within cells in response to this lipopeptide, which are involved in stress response, carbohydrate, amino acid and nucleotide metabolism, cellular component organization, calcium homeostasis, protein degradation, RNA processing, gene transcription, and others, suggesting that, in addition to inducing cell membrane permeabilization, iturin exhibits antibiotic activities by targeting intracellular molecules. Based on these results, a model of action of bacillomycin L against R. solani hyphal cells was proposed. Our study provides new insight into the antibiotic mechanisms of iturins. PMID:27267622

  11. Infrared film for aerial photography

    USGS Publications Warehouse

    Anderson, William H.

    1979-01-01

    Considerable interest has developed recently in the use of aerial photographs for agricultural management. Even the simplest hand-held aerial photographs, especially those taken with color infrared film, often provide information not ordinarily available through routine ground observation. When fields are viewed from above, patterns and variations become more apparent, often allowing problems to be spotted which otherwise may go undetected.

  12. Carlactone-type strigolactones and their synthetic analogues as inducers of hyphal branching in arbuscular mycorrhizal fungi.

    PubMed

    Mori, Narumi; Nishiuma, Kenta; Sugiyama, Takuya; Hayashi, Hideo; Akiyama, Kohki

    2016-10-01

    Hyphal branching in the vicinity of host roots is a host recognition response of arbuscular mycorrhizal fungi. This morphological event is elicited by strigolactones. Strigolactones are carotenoid-derived terpenoids that are synthesized from carlactone and its oxidized derivatives. To test the possibility that carlactone and its oxidized derivatives might act as host-derived precolonization signals in arbuscular mycorrhizal symbiosis, carlactone, carlactonoic acid, and methyl carlactonoate as well as monohydroxycarlactones, 4-, 18-, and 19-hydroxycarlactones, were synthesized chemically and evaluated for hyphal branching-inducing activity in germinating spores of the arbuscular mycorrhizal fungus Gigaspora margarita. Hyphal branching activity was found to correlate with the degree of oxidation at C-19 methyl. Carlactone was only weakly active (100 ng/disc), whereas carlactonoic acid showed comparable activity to the natural canonical strigolactones such as strigol and sorgomol (100 pg/disc). Hydroxylation at either C-4 or C-18 did not significantly affect the activity. A series of carlactone analogues, named AD ester and AA'D diester, was synthesized by reacting formyl Meldrum's acid with benzyl, cyclohexylmethyl, and cyclogeranyl alcohols (the A-ring part), followed by coupling of the potassium enolates of the resulting formylacetic esters with the D-ring butenolide. AD ester analogues exhibited moderate activity (1 ng-100 pg/disc), while AA'D diester analogues having cyclohexylmethyl and cyclogeranyl groups were highly active on the AM fungus (10 pg/disc). These results indicate that the oxidation of methyl to carboxyl at C-19 in carlactone is a prerequisite but BC-ring formation is not essential to show hyphal branching activity comparable to that of canonical strigolactones.

  13. Garcinia xanthochymus Benzophenones Promote Hyphal Apoptosis and Potentiate Activity of Fluconazole against Candida albicans Biofilms.

    PubMed

    Jackson, Desmond N; Yang, Lin; Wu, ShiBiao; Kennelly, Edward J; Lipke, Peter N

    2015-10-01

    Xanthochymol and garcinol, isoprenylated benzophenones purified from Garcinia xanthochymus fruits, showed multiple activities against Candida albicans biofilms. Both compounds effectively prevented emergence of fungal germ tubes and were also cytostatic, with MICs of 1 to 3 μM. The compounds therefore inhibited development of hyphae and subsequent biofilm maturation. Xanthochymol treatment of developing and mature biofilms induced cell death. In early biofilm development, killing had the characteristics of apoptosis, including externalization of phosphatidyl serine and DNA fragmentation, as evidenced by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) fluorescence. These activities resulted in failure of biofilm maturation and hyphal death in mature biofilms. In mature biofilms, xanthochymol and garcinol caused the death of biofilm hyphae, with 50% effective concentrations (EC50s) of 30 to 50 μM. Additionally, xanthochymol-mediated killing was complementary with fluconazole against mature biofilms, reducing the fluconazole EC50 from >1,024 μg/ml to 13 μg/ml. Therefore, xanthochymol has potential as an adjuvant for antifungal treatments as well as in studies of fungal apoptosis.

  14. Visualization of wound periderm and hyphal profiles in pine stems inoculated with the pitch canker fungus Fusarium circinatum.

    PubMed

    Kim, Ki Woo; Lee, In Jung; Thoungchaleun, Vilakon; Kim, Chang Soo; Lee, Don Koo; Park, Eun Woo

    2009-12-01

    Postpenetration behavior of Fusarium circinatum in stems of pine species was investigated with light and transmission electron microscopy. Two-year-old stems of Pinus rigida and P. densiflora were wound-inoculated with the fungal conidial suspension and subjected to 25 degrees C for up to 30 days. It was common to observe the formation of wound periderm on each pine species, recovering wounded sites with newly formed tissues. The outermost thick layer of wound periderm was pink to red colored with the phloroglucinol-EtOH staining, indicating heavy deposition of lignin in wound periderm. The cork layers in the wound periderm of the two pine species consisted of cells that were mostly devoid of cellular contents in cytoplasm. The cork cells showed convoluted cell walls with different electron density (lamellations), which was seemingly more prevalent in P. densiflora than P. rigida. Hyphae of F. circinatum appeared normal with typical eucaryotic cytoplasm in P. rigida on ultrathin sections. Meanwhile, hyphae in P. densiflora were found to possess highly vacuolated cytoplasm, implying hyphal weakening and disintegration. Hyphal cytoplasm appeared to be a thin layer between the vacuole and the plasma membrane surrounded by cell wall. In addition, intrahyphal hyphae and concentric bodies were observed in hyphal cytoplasm. These results suggest that the architecture of wound periderm may be responsible for different responses of pine species to the invasion of F. circinatum. PMID:19484779

  15. AERIAL MEASURING SYSTEM IN JAPAN

    SciTech Connect

    Lyons, Craig; Colton, David

    2012-01-01

    The U.S. Department of Energy National Nuclear Security Agency’s Aerial Measuring System deployed personnel and equipment to partner with the U.S. Air Force in Japan to conduct multiple aerial radiological surveys. These were the first and most comprehensive sources of actionable information for U.S. interests in Japan and provided early confirmation to the government of Japan as to the extent of the release from the Fukushima Daiichi Nuclear Power Generation Station. Many challenges were overcome quickly during the first 48 hours; including installation and operation of Aerial Measuring System equipment on multiple U.S. Air Force Japan aircraft, flying over difficult terrain, and flying with talented pilots who were unfamiliar with the Aerial Measuring System flight patterns. These all combined to make for a dynamic and non-textbook situation. In addition, the data challenges of the multiple and on-going releases, and integration with the Japanese government to provide valid aerial radiological survey products that both military and civilian customers could use to make informed decisions, was extremely complicated. The Aerial Measuring System Fukushima response provided insight in addressing these challenges and gave way to an opportunity for the expansion of the Aerial Measuring System’s mission beyond the borders of the US.

  16. Aerial thermography for energy conservation

    NASA Technical Reports Server (NTRS)

    Jack, J. R.

    1978-01-01

    Thermal infrared scanning from an aircraft is a convenient and commercially available means for determining relative rates of energy loss from building roofs. The need to conserve energy as fuel costs makes the mass survey capability of aerial thermography an attractive adjunct to community energy awareness programs. Background information on principles of aerial thermography is presented. Thermal infrared scanning systems, flight and environmental requirements for data acquisition, preparation of thermographs for display, major users and suppliers of thermography, and suggested specifications for obtaining aerial scanning services were reviewed.

  17. Influence of Hyphal Inoculum potential on the Competitive Success of Fungi Colonizing Wood.

    PubMed

    Song, Zewei; Vail, Andrew; Sadowsky, Michael J; Schilling, Jonathan S

    2015-05-01

    The relative amounts of hyphal inoculum in forest soils may determine the capacity for fungi to compete with and replace early colonizers of wood in ground contact. Our aim in this study was to test the flexibility of priority effects (colonization timing) by varying the timing of inoculum introduction (i.e., precolonization) and amount of inoculum (i.e., inoculum potential). We controlled these variables in soil-block microcosms using fungi with known competitive outcomes in similar conditions, tracking isolate-specific fungal biomass, and residue physiochemistry over time. In the precolonization trial (experiment I), a brown rot fungus Gloeophyllum trabeum was given 1, 3, or 5 weeks to precolonize wood blocks (oak, birch, pine, and spruce) prior the introduction of a white rot fungus, Irpex lacteus, a more aggressive colonizer in this set-up. In the inoculum potential trial (experiment II), the fungi were inoculated simultaneously, but with eightfold higher brown rot inoculum than that of experiment I. As expected, longer precolonization duration increased the chance for the less-competitive brown rot fungus to outcompete its white rot opponent. Higher brown rot fungal inoculum outside of the wood matrix also resulted in competitive success for the brown rot isolate in most cases. These temporal shifts in fungal dominance were detectable in a 'community snapshot' as isolate-specific quantitative PCR, but also as functionally-relevant consequences of wood rot type, including carbohydrate depolymerization and pH. These results from a controlled system reinforce fungal-fungal interaction and suggest that relative inoculum availability beyond the wood matrix (i.e., soils) might regulate the duration of priority effects and shift the functional trajectory of wood decomposition.

  18. Subcompartmentalization by cross-membranes during early growth of Streptomyces hyphae

    PubMed Central

    Yagüe, Paula; Willemse, Joost; Koning, Roman I.; Rioseras, Beatriz; López-García, María T.; Gonzalez-Quiñonez, Nathaly; Lopez-Iglesias, Carmen; Shliaha, Pavel V.; Rogowska-Wrzesinska, Adelina; Koster, Abraham J.; Jensen, Ole N.; van Wezel, Gilles P.; Manteca, Ángel

    2016-01-01

    Bacteria of the genus Streptomyces are a model system for bacterial multicellularity. Their mycelial life style involves the formation of long multinucleated hyphae during vegetative growth, with occasional cross-walls separating long compartments. Reproduction occurs by specialized aerial hyphae, which differentiate into chains of uninucleoid spores. While the tubulin-like FtsZ protein is required for the formation of all peptidoglycan-based septa in Streptomyces, canonical divisome-dependent cell division only occurs during sporulation. Here we report extensive subcompartmentalization in young vegetative hyphae of Streptomyces coelicolor, whereby 1 μm compartments are formed by nucleic acid stain-impermeable barriers. These barriers possess the permeability properties of membranes and at least some of them are cross-membranes without detectable peptidoglycan. Z-ladders form during the early growth, but cross-membrane formation does not depend on FtsZ. Thus, a new level of hyphal organization is presented involving unprecedented high-frequency compartmentalization, which changes the old dogma that Streptomyces vegetative hyphae have scarce compartmentalization. PMID:27514833

  19. Subcompartmentalization by cross-membranes during early growth of Streptomyces hyphae.

    PubMed

    Yagüe, Paula; Willemse, Joost; Koning, Roman I; Rioseras, Beatriz; López-García, María T; Gonzalez-Quiñonez, Nathaly; Lopez-Iglesias, Carmen; Shliaha, Pavel V; Rogowska-Wrzesinska, Adelina; Koster, Abraham J; Jensen, Ole N; van Wezel, Gilles P; Manteca, Ángel

    2016-01-01

    Bacteria of the genus Streptomyces are a model system for bacterial multicellularity. Their mycelial life style involves the formation of long multinucleated hyphae during vegetative growth, with occasional cross-walls separating long compartments. Reproduction occurs by specialized aerial hyphae, which differentiate into chains of uninucleoid spores. While the tubulin-like FtsZ protein is required for the formation of all peptidoglycan-based septa in Streptomyces, canonical divisome-dependent cell division only occurs during sporulation. Here we report extensive subcompartmentalization in young vegetative hyphae of Streptomyces coelicolor, whereby 1 μm compartments are formed by nucleic acid stain-impermeable barriers. These barriers possess the permeability properties of membranes and at least some of them are cross-membranes without detectable peptidoglycan. Z-ladders form during the early growth, but cross-membrane formation does not depend on FtsZ. Thus, a new level of hyphal organization is presented involving unprecedented high-frequency compartmentalization, which changes the old dogma that Streptomyces vegetative hyphae have scarce compartmentalization. PMID:27514833

  20. Subcompartmentalization by cross-membranes during early growth of Streptomyces hyphae.

    PubMed

    Yagüe, Paula; Willemse, Joost; Koning, Roman I; Rioseras, Beatriz; López-García, María T; Gonzalez-Quiñonez, Nathaly; Lopez-Iglesias, Carmen; Shliaha, Pavel V; Rogowska-Wrzesinska, Adelina; Koster, Abraham J; Jensen, Ole N; van Wezel, Gilles P; Manteca, Ángel

    2016-08-12

    Bacteria of the genus Streptomyces are a model system for bacterial multicellularity. Their mycelial life style involves the formation of long multinucleated hyphae during vegetative growth, with occasional cross-walls separating long compartments. Reproduction occurs by specialized aerial hyphae, which differentiate into chains of uninucleoid spores. While the tubulin-like FtsZ protein is required for the formation of all peptidoglycan-based septa in Streptomyces, canonical divisome-dependent cell division only occurs during sporulation. Here we report extensive subcompartmentalization in young vegetative hyphae of Streptomyces coelicolor, whereby 1 μm compartments are formed by nucleic acid stain-impermeable barriers. These barriers possess the permeability properties of membranes and at least some of them are cross-membranes without detectable peptidoglycan. Z-ladders form during the early growth, but cross-membrane formation does not depend on FtsZ. Thus, a new level of hyphal organization is presented involving unprecedented high-frequency compartmentalization, which changes the old dogma that Streptomyces vegetative hyphae have scarce compartmentalization.

  1. MVE1 Encoding the velvet gene product homolog in Mycosphaerella graminicola is associated with aerial mycelium formation, melanin biosynthesis, hyphal swelling, and light signaling

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The ascomycete fungus Mycosphaerella graminicola is an important pathogen of wheat that causes the disease septoria tritici blotch. Despite the serious impact of M. graminicola on wheat production worldwide, knowledge about its molecular biology is limited. The velvet gene, veA, is one of the key re...

  2. Modeling aerial refueling operations

    NASA Astrophysics Data System (ADS)

    McCoy, Allen B., III

    Aerial Refueling (AR) is the act of offloading fuel from one aircraft (the tanker) to another aircraft (the receiver) in mid flight. Meetings between tanker and receiver aircraft are referred to as AR events and are scheduled to: escort one or more receivers across a large body of water; refuel one or more receivers; or train receiver pilots, tanker pilots, and boom operators. In order to efficiently execute the Aerial Refueling Mission, the Air Mobility Command (AMC) of the United States Air Force (USAF) depends on computer models to help it make tanker basing decisions, plan tanker sorties, schedule aircraft, develop new organizational doctrines, and influence policy. We have worked on three projects that have helped AMC improve its modeling and decision making capabilities. Optimal Flight Planning. Currently Air Mobility simulation and optimization software packages depend on algorithms which iterate over three dimensional fuel flow tables to compute aircraft fuel consumption under changing flight conditions. When a high degree of fidelity is required, these algorithms use a large amount of memory and CPU time. We have modeled the rate of aircraft fuel consumption with respect to AC GrossWeight, Altitude and Airspeed. When implemented, this formula will decrease the amount of memory and CPU time needed to compute sortie fuel costs and cargo capacity values. We have also shown how this formula can be used in optimal control problems to find minimum costs flight plans. Tanker Basing Demand Mismatch Index. Since 1992, AMC has relied on a Tanker Basing/AR Demand Mismatch Index which aggregates tanker capacity and AR demand data into six regions. This index was criticized because there were large gradients along regional boundaries. Meanwhile tankers frequently cross regional boundaries to satisfy the demand for AR support. In response we developed continuous functions to score locations with respect to their proximity to demand for AR support as well as their

  3. Interactions between Streptomyces coelicolor and Bacillus subtilis: Role of Surfactants in Raising Aerial Structures

    PubMed Central

    Straight, Paul D.; Willey, Joanne M.; Kolter, Roberto

    2006-01-01

    Using mixed-species cultures, we have undertaken a study of interactions between two common spore-forming soil bacteria, Bacillus subtilis and Streptomyces coelicolor. Our experiments demonstrate that the development of aerial hyphae and spores by S. coelicolor is inhibited by surfactin, a lipopeptide surfactant produced by B. subtilis. Current models of aerial development by sporulating bacteria and fungi postulate a role for surfactants in reducing surface tension at air-liquid interfaces, thereby removing the major barrier to aerial growth. S. coelicolor produces SapB, an amphipathic peptide that is surface active and required for aerial growth on certain media. Loss of aerial hyphae in developmental mutants can be rescued by addition of purified SapB. While a surfactant from a fungus can substitute for SapB in a mutant that lacks aerial hyphae, not all surfactants have this effect. We show that surfactin is required for formation of aerial structures on the surface of B. subtilis colonies. However, in contrast to this positive role, our experiments reveal that surfactin acts antagonistically by arresting S. coelicolor aerial development and causing altered expression of developmental genes. Our observations support the idea that surfactants function specifically for a given organism regardless of their shared ability to reduce surface tension. Production of surfactants with antagonistic activity could provide a powerful competitive advantage during surface colonization and in competition for resources. PMID:16788200

  4. Dynamics of aerial target pursuit

    NASA Astrophysics Data System (ADS)

    Pal, S.

    2015-12-01

    During pursuit and predation, aerial species engage in multitasking behavior that involve simultaneous target detection, tracking, decision-making, approach and capture. The mobility of the pursuer and the target in a three dimensional environment during predation makes the capture task highly complex. Many researchers have studied and analyzed prey capture dynamics in different aerial species such as insects and bats. This article focuses on reviewing the capture strategies adopted by these species while relying on different sensory variables (vision and acoustics) for navigation. In conclusion, the neural basis of these capture strategies and some applications of these strategies in bio-inspired navigation and control of engineered systems are discussed.

  5. AERIAL OF VEHICLE ASSEMBLY BUILDING & SURROUNDING AREA

    NASA Technical Reports Server (NTRS)

    1977-01-01

    AERIAL OF VEHICLE ASSEMBLY BUILDING & SURROUNDING AREA KSC-377C-0082.41 116-KSC-377C-82.41, P-15877, ARCHIVE-04151 Aerial view - Shuttle construction progress - VAB and Orbiter Processing Facilities - direction northwest.

  6. Floating aerial LED signage based on aerial imaging by retro-reflection (AIRR).

    PubMed

    Yamamoto, Hirotsugu; Tomiyama, Yuka; Suyama, Shiro

    2014-11-01

    We propose a floating aerial LED signage technique by utilizing retro-reflection. The proposed display is composed of LEDs, a half mirror, and retro-reflective sheeting. Directivity of the aerial image formation and size of the aerial image have been investigated. Furthermore, a floating aerial LED sign has been successfully formed in free space.

  7. Reconnaissance mapping from aerial photographs

    NASA Technical Reports Server (NTRS)

    Weeden, H. A.; Bolling, N. B. (Principal Investigator)

    1975-01-01

    The author has identified the following significant results. Engineering soil and geology maps were successfully made from Pennsylvania aerial photographs taken at scales from 1:4,800 to 1:60,000. The procedure involved a detailed study of a stereoscopic model while evaluating landform, drainage, erosion, color or gray tones, tone and texture patterns, vegetation, and cultural or land use patterns.

  8. Aerial photography for sensing plant anomalies

    NASA Technical Reports Server (NTRS)

    Gausman, H. W.; Cardenas, R.; Hart, W. G.

    1970-01-01

    Changes in the red tonal response of Kodak Ektrachrome Infrared Aero 8443 film (EIR) are often incorrectly attributed solely to variations in infrared light reflectance of plant leaves, when the primary influence is a difference in visible light reflectance induced by varying chlorophyll contents. Comparisons are made among aerial photographic images of high- and low-chlorophyll foliage. New growth, foot rot, and boron and chloride nutrient toxicites produce low-chlorophyll foliage, and EIR transparency images of light red or white compared with dark-red images of high-chlorophyll foliage. Deposits of the sooty mold fungus that subsists on the honeydew produced by brown soft scale insects, obscure the citrus leaves' green color. Infected trees appear as black images on EIR film transparencies compared with red images of healthy trees.

  9. MoARG1, MoARG5,6 and MoARG7 involved in arginine biosynthesis are essential for growth, conidiogenesis, sexual reproduction, and pathogenicity in Magnaporthe oryzae.

    PubMed

    Zhang, Yong; Shi, Huanbin; Liang, Shuang; Ning, Guoao; Xu, Nanchang; Lu, Jianping; Liu, Xiaohong; Lin, Fucheng

    2015-11-01

    Arginine is one of the most versatile amino acids in eukaryote cells, which plays important roles in a multitude of processes such as protein synthesis, nitrogen metabolism, nitric oxide (NO) and urea biosynthesis. The de novo arginine biosynthesis pathway is conserved among fungal kingdom, but poorly understood in plant pathogenic fungi. Here, we characterized the functions of three synthetic enzyme-encoding genes MoARG1, MoARG5,6, and MoARG7, which involved the seventh step, second-third step and fifth step of arginine biosynthesis in Magnaporthe oryzae, respectively. Deletion of MoARG1 or MoARG5,6, resulted in arginine auxotrophic mutants, which had a strict requirement for arginine on minimal medium (MM). Both ΔMoarg1 and ΔMoarg5,6 severely reduced in aerial hyphal growth, pigmentation, conidiogenesis, sexual reproduction and pathogenicity. Interestingly, like Saccharomyces cerevisiae, deletion of MoARG7 caused a leaky arginine auxotrophy, and attenuated pathogenicity. Limited appressorium-mediated penetration and restricted invasive hyphae growth in host cells are responsible for the severely attenuated pathogenicity of the Arg(-) mutants. Additionally, we monitored the NO generation during conidial germination and appressorial formation in both Arg(-) mutants and wild type, and demonstrated that NO generation may not occur via arginine-dependent pathway in M. oryzae. In summary, MoARG1, MoARG5,6, and MoARG7 are required for growth, conidiogenesis, sexual reproduction, and pathogenicity in M. oryzae.

  10. Dissolved oxygen levels affect dimorphic growth by the entomopathogenic fungus Isaria fumosorosea

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The entomopathogenic fungus Isaria fumosorosea is capable of dimorphic growth (hyphal or yeast-like) in submerged culture. In shake flask studies, we evaluated the impact of aeration on the mode of growth of I. fumosorosea. Using 250 mL baffled Erlenmeyer flasks, culture volumes of 50, 100, 150, a...

  11. Aerial Photography: Use in Detecting Simulated Insect Defoliation in Corn

    NASA Technical Reports Server (NTRS)

    Chiang, H. C.; Latham, R.; Meyer, M. P.

    1973-01-01

    Artificial defoliation in corn was used to explore the usefulness of aerial photography in detecting crop insect infestations. Defoliation on the top of plants was easily detected, while that on the base was less so. Aero infrared film with Wratten 89B filter gave the best results, and morning flights at the scale of 1:15840 are recommended. Row direction, plant growth stage, and time elapse since defoliation were not important factors.

  12. Aerial Photographs and Satellite Images

    USGS Publications Warehouse

    ,

    1997-01-01

    Photographs and other images of the Earth taken from the air and from space show a great deal about the planet's landforms, vegetation, and resources. Aerial and satellite images, known as remotely sensed images, permit accurate mapping of land cover and make landscape features understandable on regional, continental, and even global scales. Transient phenomena, such as seasonal vegetation vigor and contaminant discharges, can be studied by comparing images acquired at different times. The U.S. Geological Survey (USGS), which began using aerial photographs for mapping in the 1930's, archives photographs from its mapping projects and from those of some other Federal agencies. In addition, many images from such space programs as Landsat, begun in 1972, are held by the USGS. Most satellite scenes can be obtained only in digital form for use in computer-based image processing and geographic information systems, but in some cases are also available as photographic products.

  13. Aerial robotic data acquisition system

    SciTech Connect

    Hofstetter, K.J.; Hayes, D.W.; Pendergast, M.M.; Corban, J.E.

    1993-12-31

    A small, unmanned aerial vehicle (UAV), equipped with sensors for physical and chemical measurements of remote environments, is described. A miniature helicopter airframe is used as a platform for sensor testing and development. The sensor output is integrated with the flight control system for real-time, interactive, data acquisition and analysis. Pre-programmed flight missions will be flown with several sensors to demonstrate the cost-effective surveillance capabilities of this new technology.

  14. Telemetry of Aerial Radiological Measurements

    SciTech Connect

    H. W. Clark, Jr.

    2002-10-01

    Telemetry has been added to National Nuclear Security Administration's (NNSA's) Aerial Measuring System (AMS) Incident Response aircraft to accelerate availability of aerial radiological mapping data. Rapid aerial radiological mapping is promptly performed by AMS Incident Response aircraft in the event of a major radiological dispersal. The AMS airplane flies the entire potentially affected area, plus a generous margin, to provide a quick look at the extent and severity of the event. The primary result of the AMS Incident Response over flight is a map of estimated exposure rate on the ground along the flight path. Formerly, it was necessary to wait for the airplane to land before the map could be seen. Now, while the flight is still in progress, data are relayed via satellite directly from the aircraft to an operations center, where they are displayed and disseminated. This permits more timely utilization of results by decision makers and redirection of the mission to optimize its value. The current telemetry capability can cover all of North America. Extension to a global capability is under consideration.

  15. Automated recognition of forest patterns using aerial photographs

    NASA Astrophysics Data System (ADS)

    Barbezat, Vincent; Kreiss, Philippe; Sulzmann, Armin; Jacot, Jacques

    1996-12-01

    In Switzerland, aerial photos are indispensable tools for research into ecosystems and their management. Every six years since 1950, the whole of Switzerland has been systematically surveyed by aerial photos. In the forestry field, these documents not only provide invaluable information but also give support to field activities such as the drawing up of tree population maps, intervention planning, precise positioning of the upper forest limit, evaluation of forest damage and rates of tree growth. Up to now, the analysis of aerial photos has been carried out by specialists who painstakingly examine every photograph, which makes it a very long, exacting and expensive job. The IMT-DMT of the EPFL and Antenne romande of FNP, aware of the special interest involved and the necessity of automated classification of aerial photos, have pooled their resources to develop a software program capable of differentiating between single trees, copses and dense forests. The developed algorithms detect the crowns of the trees and the surface of the orthogonal projection. Form the shadow of each tree they calculate its height. They also determine the position of the tree in the Swiss national coordinate thanks to the implementation of a numeric altitude model. For the future, we have the prospect of many new and better uses of aerial photos being available to us, particularly where isolated stands are concerned and also when evolutions based on a diachronic series of photos have to be assessed: from timberline monitoring in the research on global change to the exploitation of wooded pastures on small surface areas.

  16. Cytosolic streaming in vegetative mycelium and aerial structures of Aspergillus niger

    PubMed Central

    Bleichrodt, R.; Vinck, A.; Krijgsheld, P.; van Leeuwen, M.R.; Dijksterhuis, J.; Wösten, H.A.B.

    2013-01-01

    Aspergillus niger forms aerial hyphae and conidiophores after a period of vegetative growth. The hyphae within the mycelium of A. niger are divided by septa. The central pore in these septa allows for cytoplasmic streaming. Here, we studied inter- and intra-compartmental streaming of the reporter protein GFP in A. niger. Expression of the gene encoding nuclear targeted GFP from the gpdA or glaA promoter resulted in strong fluorescence of nuclei within the vegetative hyphae and weak fluorescence in nuclei within the aerial structures. These data and nuclear run on experiments showed that gpdA and glaA are higher expressed in the vegetative mycelium when compared to aerial hyphae, conidiophores and conidia. Notably, gpdA or glaA driven expression of the gene encoding cytosolic GFP resulted in strongly fluorescent vegetative hyphae and aerial structures. Apparently, GFP streams from vegetative hyphae into aerial structures. This was confirmed by monitoring fluorescence of photo-activatable GFP (PA-GFP). In contrast, PA-GFP did not stream from aerial structures to vegetative hyphae. Streaming of PA-GFP within vegetative hyphae or within aerial structures of A. niger occurred at a rate of 10–15 μm s-1. Taken together, these results not only show that GFP streams from the vegetative mycelium to aerial structures but it also indicates that its encoding RNA is not streaming. Absence of RNA streaming would explain why distinct RNA profiles were found in aerial structures and the vegetative mycelium by nuclear run on analysis and micro-array analysis. PMID:23450745

  17. A Spherical Aerial Terrestrial Robot

    NASA Astrophysics Data System (ADS)

    Dudley, Christopher J.

    This thesis focuses on the design of a novel, ultra-lightweight spherical aerial terrestrial robot (ATR). The ATR has the ability to fly through the air or roll on the ground, for applications that include search and rescue, mapping, surveillance, environmental sensing, and entertainment. The design centers around a micro-quadcopter encased in a lightweight spherical exoskeleton that can rotate about the quadcopter. The spherical exoskeleton offers agile ground locomotion while maintaining characteristics of a basic aerial robot in flying mode. A model of the system dynamics for both modes of locomotion is presented and utilized in simulations to generate potential trajectories for aerial and terrestrial locomotion. Details of the quadcopter and exoskeleton design and fabrication are discussed, including the robot's turning characteristic over ground and the spring-steel exoskeleton with carbon fiber axle. The capabilities of the ATR are experimentally tested and are in good agreement with model-simulated performance. An energy analysis is presented to validate the overall efficiency of the robot in both modes of locomotion. Experimentally-supported estimates show that the ATR can roll along the ground for over 12 minutes and cover the distance of 1.7 km, or it can fly for 4.82 minutes and travel 469 m, on a single 350 mAh battery. Compared to a traditional flying-only robot, the ATR traveling over the same distance in rolling mode is 2.63-times more efficient, and in flying mode the system is only 39 percent less efficient. Experimental results also demonstrate the ATR's transition from rolling to flying mode.

  18. Polyoxin D inhibits growth of zoopathogenic fungi.

    PubMed Central

    Becker, J M; Covert, N L; Shenbagamurthi, P; Steinfeld, A S; Naider, F

    1983-01-01

    We demonstrated that polyoxin D at millimolar concentrations caused marked morphological alterations of the human pathogens Candida albicans and Cryptococcus neoformans. C. albicans incubated in the presence of this drug grew in long chains that were severely swollen. Polyoxin D inhibited the growth of C. neoformans and killed cells of both the yeast and the hyphal phase of C. albicans. These observations give the first evidence that polyoxin antibiotics can kill zoopathogenic fungi. Images PMID:6351734

  19. Recent advances in genes involved in secondary metabolite synthesis, hyphal development, energy metabolism and pathogenicity in Fusarium graminearum (teleomorph Gibberella zeae).

    PubMed

    Geng, Zongyi; Zhu, Wei; Su, Hao; Zhao, Yong; Zhang, Ke-Qin; Yang, Jinkui

    2014-01-01

    The ascomycete fungus, Fusarium graminearum (teleomorph Gibberella zeae), is the most common causal agent of Fusarium head blight (FHB), a devastating disease for cereal crops worldwide. F. graminearum produces ascospores (sexual spores) and conidia (asexual spores), which can serve as disease inocula of FHB. Meanwhile, Fusarium-infected grains are often contaminated with mycotoxins such as trichothecenes (TRIs), fumonisins, and zearalenones, among which TRIs are related to the pathogenicity of F. graminearum, and these toxins are hazardous to humans and livestock. In recent years, with the complete genome sequencing of F. graminearum, an increasing number of functional genes involved in the production of secondary metabolites, hyphal differentiation, sexual and asexual reproduction, virulence and pathogenicity have been identified from F. graminearum. In this review, the secondary metabolite synthesis, hyphal development and pathogenicity related genes in F. graminearum were thoroughly summarized, and the genes associated with secondary metabolites, sexual reproduction, energy metabolism, and pathogenicity were highlighted.

  20. Calcium-induced conidiation in Penicillium cyclopium: calcium triggers cytosolic alkalinization at the hyphal tip.

    PubMed Central

    Roncal, T; Ugalde, U O; Irastorza, A

    1993-01-01

    Addition of Ca2+ (1 to 10 mM) to submerged cultures of Penicillium cyclopium induces conidiation. Ca2+ induced an increase in cytosolic pH from approximately 7.00 to > 7.60 in less than 10 min, as determined with the fluorescent pH probe fluorescein. Measurement of the H(+)-ATPase activity in total membrane fractions did not show any stable activation in vivo as a result of Ca2+ treatment. By fluorescence ratio imaging microscopy, it was observed that vegetative hyphae exhibit a tip-to-base pH gradient, with the tip being more acidic. Ca2+ caused this gradient to dissipate within 10 min. The effect of several agents that are supposed to cause internal acidification, by different means, on conidiation was tested. Concentrations of these agents that did not significantly affect growth but inhibited Ca(2+)-induced conidiation also prevented the intracellular alkalinization observed after exposure to the cation. Calcium channel blockers (lanthanum, cobalt, verapamil, and nifedipine) were not able to inhibit Ca(2+)-induced conidiation, although their effect on calcium uptake was not evaluated. However, the combined results point towards externally bound Ca2+ as the primary agent of conidiation induction, causing changes in plasma membrane function which disrupt the pH gradient observed during apical growth. Images PMID:8380805

  1. Astronomical Methods in Aerial Navigation

    NASA Technical Reports Server (NTRS)

    Beij, K Hilding

    1925-01-01

    The astronomical method of determining position is universally used in marine navigation and may also be of service in aerial navigation. The practical application of the method, however, must be modified and adapted to conform to the requirements of aviation. Much of this work of adaptation has already been accomplished, but being scattered through various technical journals in a number of languages, is not readily available. This report is for the purpose of collecting under one cover such previous work as appears to be of value to the aerial navigator, comparing instruments and methods, indicating the best practice, and suggesting future developments. The various methods of determining position and their application and value are outlined, and a brief resume of the theory of the astronomical method is given. Observation instruments are described in detail. A complete discussion of the reduction of observations follows, including a rapid method of finding position from the altitudes of two stars. Maps and map cases are briefly considered. A bibliography of the subject is appended.

  2. Isolated root caps, border cells, and mucilage from host roots stimulate hyphal branching of the arbuscular mycorrhizal fungus, Gigaspora gigantea.

    PubMed

    Nagahashi, Gerald; Douds, David D

    2004-09-01

    Unlike previous reports that have shown that water soluble and volatile compounds from roots or root exudates play an important role in precolonization events during arbuscular mycorrhizal (AM) fungus-host root interactions (Bécard & Piché 1989, Giovannetti et al. 1993), the results shown here deal with particulate and viscous fractions isolated from host roots. Root caps and a slow sedimenting particulate fraction (SSPF) were rapidly isolated and separated from Ri T-DNA transformed carrot roots (D. carota) grown in liquid culture. In addition, border cells (BC) and mucilage were isolated from aseptically grown corn seedlings (Zea mays). Root caps, SSPF (composed mainly of small root cap fragments and some BCs), BCs, and mucilage all had an associated AM fungus hyphal branching stimulator. Root caps stored for 5 d at 4 degrees C appeared to either synthesize or slowly release the branching stimulator. Also, isolated root caps from roots grown in the absence of P contained more branch stimulating activity than those isolated from roots grown in the presence of P. Although the branching stimulation activity in particulate fractions was low compared to that of the exudate, the particulate fractions can stick to the root surface at considerable distances from the root tip. This may be significant during the infection and colonization of host roots at sites far removed from the primary location of exudation.

  3. Physiological role of germicidins in spore germination and hyphal elongation in Streptomyces coelicolor A3(2).

    PubMed

    Aoki, Yuu; Matsumoto, Daisuke; Kawaide, Hiroshi; Natsume, Masahiro

    2011-09-01

    Four germicidin homologs were isolated from a liquid culture of Streptomyces coelicolor A3(2). These were identified as germicidins A, B and C, and surugapyrone A (germicidin D). Absolute stereochemistry of the chiral center in germicidins A and C is determined to be S. All germicidins inhibited germination of S. coelicolor A3(2) spores above 1 μg ml(-1). S. coelicolor A3(2) spores collected from a single petri dish (9 cm i.d.) contained 5.4 μg of germicidin A (-2.7 × 10(-14) g per spore), which accounts for 2.3% of the spore extract, and contents of germicidins B, C and D were 0.2-0.8 μg. The activity of the spore extract corresponded well with the sum of the activity of each germicidin, which was estimated from the content and dose-response curve, which indicates that germicidins functions as self-germination inhibitors in S. coelicolor A3(2). Inhibitory action of germicidin A on spore germination was reversible and germicidin A inhibited not only spore germination but also hyphal elongation.

  4. Candida albicans Dbf4-dependent Cdc7 kinase plays a novel role in the inhibition of hyphal development

    PubMed Central

    Lai, Wei-Chung; Chang, Tschen-wei; Wu, Chang Hao; Yang, Shu-Ya; Lee, Tai-Lin; Li, Wan Chen; Chien, Ting; Cheng, Yu-Che; Shieh, Jia-Ching

    2016-01-01

    Candida albicans is an opportunistic human fungal pathogen. The ability to switch among multiple cellular forms is key to its pathogenesis. The Dbf4-dependent protein kinase gene CDC7 is conserved due to its role in initiating DNA replication. Because a C. albicans Cdc7 (Cacdc7) homozygous null was not viable, we generated a C. albicans strain with a deleted C. albicans CDC7 (CaCDC7) allele and an expression-repressible allele. Surprisingly, cells of the strain grew as hyphae under the repressed conditions. The in vitro kinase assays confirmed that CaCdc7 (K232) and CaCdc7 (T437) are critical for catalytic and phosphoacceptor of activation activity, respectively. C. albicans cells formed hyphae when expressing either the catalytically inactive CaCdc7 (K232R) or the phosphoacceptor-deficient CaCdc7 (T437A). While CaCdc7 interacted with CaDbf4, cells of the strain in which CaCDC7 was repressed were not rescued by constitutively expressing C. albicans DBF4 or vice versa. We conclude that CaDBF4-dependent CaCDC7 is an essential gene suppressing the hyphal development. PMID:27644158

  5. Early differential molecular response of a macrophage cell line to yeast and hyphal forms of Candida albicans.

    PubMed Central

    Blasi, E; Pitzurra, L; Puliti, M; Lanfrancone, L; Bistoni, F

    1992-01-01

    The dimorphic transition of Candida albicans from the yeast (Y-Candida) to the hyphal (H-Candida) form is a complex event; the relevance of this transition in fungal pathogenicity is still poorly understood. By using a cloned macrophage cell line (ANA-1), we questioned whether the interaction between macrophages and Y-Candida or H-Candida could affect specific cell functions, i.e., tumor necrosis factor and lysozyme production. We found that ANA-1 macrophages selectively responded to H-Candida with increased tumor necrosis factor and downregulated lysozyme, as assessed by measurement of relative mRNA levels and secreted biological activities. The H-Candida-mediated effects were (i) dependent upon the ratio between ANA-1 macrophages and H-Candida, (ii) detectable after 1 h of coincubation, and (iii) accomplished without fungal ingestion. Conversely, Y-Candida, which was found inside the ANA-1 macrophages, did not affect tumor necrosis factor and lysozyme production, nor did it prevent the macrophage response to other stimuli. Overall, these results indicate that a macrophage can distinguish between Y-Candida and H-Candida and that only the latter is able to modulate specific functions. H-Candida is recognized and probably processed as an extracellular target. The possible implication of macrophages as autocrine and paracrine regulatory cells during Candida infections is discussed. Images PMID:1541557

  6. FgFlbD regulates hyphal differentiation required for sexual and asexual reproduction in the ascomycete fungus Fusarium graminearum.

    PubMed

    Son, Hokyoung; Kim, Myung-Gu; Chae, Suhn-Kee; Lee, Yin-Won

    2014-11-01

    Fusarium graminearum is a filamentous fungal plant pathogen that infects major cereal crops. The fungus produces both sexual and asexual spores in order to endure unfavorable environmental conditions and increase their numbers and distribution across plants. In a model filamentous fungus, Aspergillus nidulans, early induction of conidiogenesis is orchestrated by the fluffy genes. The objectives of this study were to characterize fluffy gene homologs involved in conidiogenesis and their mechanism of action in F. graminearum. We characterized five fluffy gene homologs in F. graminearum and found that FlbD is the only conserved regulator for conidiogenesis in A. nidulans and F. graminearum. Deletion of fgflbD prevented hyphal differentiation and the formation of perithecia. Successful interspecies complementation using A. nidulans flbD demonstrated that the molecular mechanisms responsible for FlbD functions are conserved in F. graminearum. Moreover, abaA-wetA pathway is positively regulated by FgFlbD during conidiogenesis in F. graminearum. Deleting fgflbD abolished morphological effects of abaA overexpression, which suggests that additional factors for FgFlbD or an AbaA-independent pathway for conidiogenesis are required for F. graminearum conidiation. Importantly, this study led to the construction of a genetic pathway of F. graminearum conidiogenesis and provides new insights into the genetics of conidiogenesis in fungi.

  7. BOREAS Level-0 ER-2 Aerial Photography

    NASA Technical Reports Server (NTRS)

    Newcomer, Jeffrey A.; Dominquez, Roseanne; Hall, Forrest G. (Editor)

    2000-01-01

    For BOReal Ecosystem-Atmosphere Study (BOREAS), the ER-2 and other aerial photography was collected to provide finely detailed and spatially extensive documentation of the condition of the primary study sites. The ER-2 aerial photography consists of color-IR transparencies collected during flights in 1994 and 1996 over the study areas.

  8. 29 CFR 1926.453 - Aerial lifts.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... accordance with 5 U.S.C. 552(a) and 1 CFR part 51. Copies may be obtained from the American National... 29 Labor 8 2011-07-01 2011-07-01 false Aerial lifts. 1926.453 Section 1926.453 Labor Regulations...) SAFETY AND HEALTH REGULATIONS FOR CONSTRUCTION Scaffolds § 1926.453 Aerial lifts. (a)...

  9. 29 CFR 1926.453 - Aerial lifts.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... accordance with 5 U.S.C. 552(a) and 1 CFR part 51. Copies may be obtained from the American National... 29 Labor 8 2014-07-01 2014-07-01 false Aerial lifts. 1926.453 Section 1926.453 Labor Regulations...) SAFETY AND HEALTH REGULATIONS FOR CONSTRUCTION Scaffolds § 1926.453 Aerial lifts. (a)...

  10. 29 CFR 1926.453 - Aerial lifts.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... accordance with 5 U.S.C. 552(a) and 1 CFR part 51. Copies may be obtained from the American National... 29 Labor 8 2010-07-01 2010-07-01 false Aerial lifts. 1926.453 Section 1926.453 Labor Regulations...) SAFETY AND HEALTH REGULATIONS FOR CONSTRUCTION Scaffolds § 1926.453 Aerial lifts. (a)...

  11. 47 CFR 32.2431 - Aerial wire.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false Aerial wire. 32.2431 Section 32.2431 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES UNIFORM SYSTEM OF ACCOUNTS FOR TELECOMMUNICATIONS COMPANIES Instructions for Balance Sheet Accounts § 32.2431 Aerial wire....

  12. 47 CFR 32.2431 - Aerial wire.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 2 2011-10-01 2011-10-01 false Aerial wire. 32.2431 Section 32.2431 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES UNIFORM SYSTEM OF ACCOUNTS FOR TELECOMMUNICATIONS COMPANIES Instructions for Balance Sheet Accounts § 32.2431 Aerial wire....

  13. A Classroom Simulation of Aerial Photography.

    ERIC Educational Resources Information Center

    Baker, Simon

    1981-01-01

    Explains how a simulation of aerial photography can help students in a college level beginning course on interpretation of aerial photography understand the interrelationships of the airplane, the camera, and the earth's surface. Procedures, objectives, equipment, and scale are discussed. (DB)

  14. Hgc1-Cdc28-how much does a single protein kinase do in the regulation of hyphal development in Candida albicans?

    PubMed

    Wang, Yue

    2016-03-01

    The fungal human pathogen Candida albicans can cause invasive infection with high mortality rates. A key virulence factor is its ability to switch between three morphologies: yeast, pseudohyphae and hyphae. In contrast to the ovalshaped unicellular yeast cells, hyphae are highly elongated, tube-like, and multicellular. A long-standing question is what coordinates all the cellular machines to construct cells with distinct shapes. Hyphal-specific genes (HSGs) are thought to hold the answer. Among the numerous HSGs found, only UME6 and HGC1 are required for hyphal development. UME6 encodes a transcription factor that regulates many HSGs including HGC1. HGC1 encodes a G1 cyclin which partners with the Cdc28 cyclin-dependent kinase. Hgc1-Cdc28 simultaneously phosphorylates and regulates multiple substrates, thus controlling multiple cellular apparatuses for morphogenesis. This review is focused on major progresses made in the past decade on Hgc1's roles and regulation in C. albicans hyphal development and other traits important for infection. PMID:26920877

  15. The regulation of methyl jasmonate on hyphal branching and GA biosynthesis in Ganoderma lucidum partly via ROS generated by NADPH oxidase.

    PubMed

    Shi, Liang; Gong, Li; Zhang, Xiangyang; Ren, Ang; Gao, Tan; Zhao, Mingwen

    2015-08-01

    Ganoderma lucidum is one of the best known medicinal basidiomycetes because it produces many pharmacologically active compounds, and methyl jasmonate (MeJA) was previously reported to induce the biosynthesis of ganoderic acids (GA) in G. lucidum. In this study, we found that MeJA not only increased the amount of GA but also increased the distance between hyphal branches by approximately 1.2-fold. Further analysis showed that MeJA could increase the intracellular ROS (reactive oxygen species) content by approximately 2.2-2.7-fold. Furthermore, the hyphal branching and GA biosynthesis regulated by MeJA treatment could be abolished by ROS scavengers to a level similar to or lower than that of the control group. These results indicated that the regulation of hyphal branching and GA biosynthesis by MeJA might occur via a ROS signaling pathway. Further analysis revealed that NADPH oxidase (NOX) plays an important role in MeJA-regulated ROS generation. Importantly, our results highlight that NOX functions in signaling cross-talk between ROS and MeJA. In addition, these findings provide an excellent opportunity to identify potential pathways linking ROS networks to MeJA signaling in fungi and suggest that plants and fungi share a conserved signaling-crosstalk mechanism.

  16. The Spitzenkörper: a choreographer of fungal growth and morphogenesis.

    PubMed

    Riquelme, Meritxell; Sánchez-León, Eddy

    2014-08-01

    The Spitzenkörper (SPK) is a multicomponent pleomorphic structure found at hyphal apices. It is necessary to maintain hyphal growth and morphogenesis in numerous fungal species, including plant and human pathogens. At the turn of the 21st century extraordinary advances in protein tagging technology and live microscopy allowed uncovering the main molecular constituents of the SPK. Distinct layers of macrovesicles and microvesicles, each carrying different cell wall synthetic enzymes, along with the actin cytoskeleton and related proteins are some of the components that make up the SPK. One of the biggest current challenges is to decipher the functional relationship between the SPK components and macromolecular complexes, such as the polarisome and the exocyst, which partially co-localize within the hyphal dome. PMID:24858006

  17. Adaptive planning of emergency aerial photogrammetric mission

    NASA Astrophysics Data System (ADS)

    Shen, Fuqiang; Zhu, Qing; Zhang, Junxiao; Miao, Shuangxi; Zhou, Xingxia; Cao, Zhenyu

    2015-12-01

    Aiming at the diversity of emergency aerial photogrammetric mission requirements, complex ground and air environmental constraints make the planning mission time-consuming. This paper presents a fast adaptation for the UAV aerial photogrammetric mission planning. First, Building emergency aerial UAVs mission the unified expression of UAVs model and mechanical model of performance parameters in the semantic space make the integrated expression of mission requirements and low altitude environment. Proposed match assessment method which based on resource and mission efficiency. Made the Adaptive match of UAV aerial resources and mission. According to the emergency aerial resource properties, considering complex air-ground environment and mission requirements constraints. Made accurate design of UAV route. Experimental results show, the method scientific and efficient, greatly enhanced the emergency response rate.

  18. Nanomechanical sensors for single microbial cell growth monitoring

    NASA Astrophysics Data System (ADS)

    Maloney, Niall; Lukacs, Gyongyi; Jensen, Jason; Hegner, Martin

    2014-06-01

    A nanomechanical technique for rapid real time detection and monitoring of microorganism growth will significantly reduce costs and diagnosis times in industrial and clinical settings. Owing to their label free detection mechanism and unprecedented sensitivity to the mass and elastic modulus of biological structures, dynamically operated cantilever arrays provide an opportunity to rapidly detect and track the evolution of microbial growth. Here we report the monitoring of the growth of single Aspergillus niger spores via the multimode response of microcantilevers. The fungal hyphal structure affects the cantilevers' nanomechanical properties as it propagates along the sensor. We demonstrate, for the first time, the mapping of cellular events with great accuracy using a cantilever frequency response. Imaging of growth conditions on the cantilever, which is performed in parallel, allows for verification of these results. Theoretical comparison and finite element modelling confirm experimental findings and allow for determination of the hyphal elastic modulus.A nanomechanical technique for rapid real time detection and monitoring of microorganism growth will significantly reduce costs and diagnosis times in industrial and clinical settings. Owing to their label free detection mechanism and unprecedented sensitivity to the mass and elastic modulus of biological structures, dynamically operated cantilever arrays provide an opportunity to rapidly detect and track the evolution of microbial growth. Here we report the monitoring of the growth of single Aspergillus niger spores via the multimode response of microcantilevers. The fungal hyphal structure affects the cantilevers' nanomechanical properties as it propagates along the sensor. We demonstrate, for the first time, the mapping of cellular events with great accuracy using a cantilever frequency response. Imaging of growth conditions on the cantilever, which is performed in parallel, allows for verification of these

  19. Differences in mechanical and structural properties of surface and aerial petioles of the aquatic plant Nymphaea odorata subsp. tuberosa (Nymphaeaceae).

    PubMed

    Etnier, Shelley A; Villani, Philip J

    2007-07-01

    Lily pads (Nymphaea odorata) exhibit heterophylly where a single plant may have leaves that are submerged, floating, or above (aerial) the surface of the water. Lily pads are placed in a unique situation because each leaf form is exposed to a distinctly different set of mechanical demands. While surface petioles may be loaded in tension under conditions of wind or waves, aerial petioles are loaded in compression because they must support the weight of the lamina. Using standard techniques, we compared the mechanical and morphological properties of both surface and aerial leaf petioles. Structural stiffness (EI) and the second moment of area (I) were higher in aerial petioles, although we detected no differences in other mechanical values (elastic modulus [E], extension ratio, and breaking strength). Morphologically, aerial petioles had a thicker rind, with increased collenchyma tissue and sclereid cell frequency. Aerial petioles also had a larger cross-sectional area and were more elliptical. Thus, subtle changes in the distribution of materials, rather than differences in their makeup, differentiate petiole forms. We suggest that the growth of aerial petioles may be an adaptive response to shading, allowing aerial leaves to rise above a crowded water surface.

  20. Differences in mechanical and structural properties of surface and aerial petioles of the aquatic plant Nymphaea odorata subsp. tuberosa (Nymphaeaceae).

    PubMed

    Etnier, Shelley A; Villani, Philip J

    2007-07-01

    Lily pads (Nymphaea odorata) exhibit heterophylly where a single plant may have leaves that are submerged, floating, or above (aerial) the surface of the water. Lily pads are placed in a unique situation because each leaf form is exposed to a distinctly different set of mechanical demands. While surface petioles may be loaded in tension under conditions of wind or waves, aerial petioles are loaded in compression because they must support the weight of the lamina. Using standard techniques, we compared the mechanical and morphological properties of both surface and aerial leaf petioles. Structural stiffness (EI) and the second moment of area (I) were higher in aerial petioles, although we detected no differences in other mechanical values (elastic modulus [E], extension ratio, and breaking strength). Morphologically, aerial petioles had a thicker rind, with increased collenchyma tissue and sclereid cell frequency. Aerial petioles also had a larger cross-sectional area and were more elliptical. Thus, subtle changes in the distribution of materials, rather than differences in their makeup, differentiate petiole forms. We suggest that the growth of aerial petioles may be an adaptive response to shading, allowing aerial leaves to rise above a crowded water surface. PMID:21636476

  1. COCOA: tracking in aerial imagery

    NASA Astrophysics Data System (ADS)

    Ali, Saad; Shah, Mubarak

    2006-05-01

    Unmanned Aerial Vehicles (UAVs) are becoming a core intelligence asset for reconnaissance, surveillance and target tracking in urban and battlefield settings. In order to achieve the goal of automated tracking of objects in UAV videos we have developed a system called COCOA. It processes the video stream through number of stages. At first stage platform motion compensation is performed. Moving object detection is performed to detect the regions of interest from which object contours are extracted by performing a level set based segmentation. Finally blob based tracking is performed for each detected object. Global tracks are generated which are used for higher level processing. COCOA is customizable to different sensor resolutions and is capable of tracking targets as small as 100 pixels. It works seamlessly for both visible and thermal imaging modes. The system is implemented in Matlab and works in a batch mode.

  2. Whitecap coverage from aerial photography

    NASA Technical Reports Server (NTRS)

    Austin, R. W.

    1970-01-01

    A program for determining the feasibility of deriving sea surface wind speeds by remotely sensing ocean surface radiances in the nonglitter regions is discussed. With a knowledge of the duration and geographical extent of the wind field, information about the conventional sea state may be derived. The use of optical techniques for determining sea state has obvious limitations. For example, such means can be used only in daylight and only when a clear path of sight is available between the sensor and the surface. However, sensors and vehicles capable of providing the data needed for such techniques are planned for the near future; therefore, a secondary or backup capability can be provided with little added effort. The information currently being sought regarding white water coverage is also of direct interest to those working with passive microwave systems, the study of energy transfer between winds and ocean currents, the aerial estimation of wind speeds, and many others.

  3. Unmanned aerial survey of elephants.

    PubMed

    Vermeulen, Cédric; Lejeune, Philippe; Lisein, Jonathan; Sawadogo, Prosper; Bouché, Philippe

    2013-01-01

    The use of a UAS (Unmanned Aircraft System) was tested to survey large mammals in the Nazinga Game Ranch in the south of Burkina Faso. The Gatewing ×100™ equipped with a Ricoh GR III camera was used to test animal reaction as the UAS passed, and visibility on the images. No reaction was recorded as the UAS passed at a height of 100 m. Observations, made on a set of more than 7000 images, revealed that only elephants (Loxodonta africana) were easily visible while medium and small sized mammals were not. The easy observation of elephants allows experts to enumerate them on images acquired at a height of 100 m. We, therefore, implemented an aerial strip sample count along transects used for the annual wildlife foot count. A total of 34 elephants were recorded on 4 transects, each overflown twice. The elephant density was estimated at 2.47 elephants/km(2) with a coefficient of variation (CV%) of 36.10%. The main drawback of our UAS was its low autonomy (45 min). Increased endurance of small UAS is required to replace manned aircraft survey of large areas (about 1000 km of transect per day vs 40 km for our UAS). The monitoring strategy should be adapted according to the sampling plan. Also, the UAS is as expensive as a second-hand light aircraft. However the logistic and flight implementation are easier, the running costs are lower and its use is safer. Technological evolution will make civil UAS more efficient, allowing them to compete with light aircraft for aerial wildlife surveys.

  4. Unmanned Aerial Survey of Elephants

    PubMed Central

    Vermeulen, Cédric; Lejeune, Philippe; Lisein, Jonathan; Sawadogo, Prosper; Bouché, Philippe

    2013-01-01

    The use of a UAS (Unmanned Aircraft System) was tested to survey large mammals in the Nazinga Game Ranch in the south of Burkina Faso. The Gatewing ×100™ equipped with a Ricoh GR III camera was used to test animal reaction as the UAS passed, and visibility on the images. No reaction was recorded as the UAS passed at a height of 100 m. Observations, made on a set of more than 7000 images, revealed that only elephants (Loxodonta africana) were easily visible while medium and small sized mammals were not. The easy observation of elephants allows experts to enumerate them on images acquired at a height of 100 m. We, therefore, implemented an aerial strip sample count along transects used for the annual wildlife foot count. A total of 34 elephants were recorded on 4 transects, each overflown twice. The elephant density was estimated at 2.47 elephants/km2 with a coefficient of variation (CV%) of 36.10%. The main drawback of our UAS was its low autonomy (45 min). Increased endurance of small UAS is required to replace manned aircraft survey of large areas (about 1000 km of transect per day vs 40 km for our UAS). The monitoring strategy should be adapted according to the sampling plan. Also, the UAS is as expensive as a second-hand light aircraft. However the logistic and flight implementation are easier, the running costs are lower and its use is safer. Technological evolution will make civil UAS more efficient, allowing them to compete with light aircraft for aerial wildlife surveys. PMID:23405088

  5. The DOE ARM Aerial Facility

    SciTech Connect

    Schmid, Beat; Tomlinson, Jason M.; Hubbe, John M.; Comstock, Jennifer M.; Mei, Fan; Chand, Duli; Pekour, Mikhail S.; Kluzek, Celine D.; Andrews, Elisabeth; Biraud, S.; McFarquhar, Greg

    2014-05-01

    The Department of Energy Atmospheric Radiation Measurement (ARM) Program is a climate research user facility operating stationary ground sites that provide long-term measurements of climate relevant properties, mobile ground- and ship-based facilities to conduct shorter field campaigns (6-12 months), and the ARM Aerial Facility (AAF). The airborne observations acquired by the AAF enhance the surface-based ARM measurements by providing high-resolution in-situ measurements for process understanding, retrieval-algorithm development, and model evaluation that are not possible using ground- or satellite-based techniques. Several ARM aerial efforts were consolidated into the AAF in 2006. With the exception of a small aircraft used for routine measurements of aerosols and carbon cycle gases, AAF at the time had no dedicated aircraft and only a small number of instruments at its disposal. In this "virtual hangar" mode, AAF successfully carried out several missions contracting with organizations and investigators who provided their research aircraft and instrumentation. In 2009, AAF started managing operations of the Battelle-owned Gulfstream I (G-1) large twin-turboprop research aircraft. Furthermore, the American Recovery and Reinvestment Act of 2009 provided funding for the procurement of over twenty new instruments to be used aboard the G-1 and other AAF virtual-hangar aircraft. AAF now executes missions in the virtual- and real-hangar mode producing freely available datasets for studying aerosol, cloud, and radiative processes in the atmosphere. AAF is also engaged in the maturation and testing of newly developed airborne sensors to help foster the next generation of airborne instruments.

  6. Aerial videotape mapping of coastal geomorphic changes

    USGS Publications Warehouse

    Debusschere, Karolien; Penland, Shea; Westphal, Karen A.; Reimer, P. Douglas; McBride, Randolph A.

    1991-01-01

    An aerial geomorphic mapping system was developed to examine the spatial and temporal variability in the coastal geomorphology of Louisiana. Between 1984 and 1990 eleven sequential annual and post-hurricane aerial videotape surveys were flown covering periods of prolonged fair weather, hurricane impacts and subsequent post-storm recoveries. A coastal geomorphic classification system was developed to map the spatial and temporal geomorphic changes between these surveys. The classification system is based on 10 years of shoreline monitoring, analysis of aerial photography for 1940-1989, and numerous field surveys. The classification system divides shorelines into two broad classes: natural and altered. Each class consists of several genetically linked categories of shorelines. Each category is further subdivided into morphologic types on the basis of landform relief, elevation, habitat type, vegetation density and type, and sediment characteristics. The classification is used with imagery from the low-altitude, high-resolution aerial videotape surveys to describe and quantify the longshore and cross-shore geomorphic, sedimentologic, and vegetative character of Louisiana's shoreline systems. The mapping system makes it possible to delineate and map detailed geomorphic habitat changes at a resolution higher than that of conventional vertical aerial photography. Morphologic units are mapped parallel to the regional shoreline from the aerial videotape imagery onto the base maps at a scale of 1:24,000. The base maps were constructed from vertical aerial photography concurrent with the data of the video imagery.

  7. Functional regions of Candida albicans hyphal cell wall protein Als3 that determine interaction with the oral bacterium Streptococcus gordonii

    PubMed Central

    Bamford, Caroline V.; Nobbs, Angela H.; Barbour, Michele E.; Lamont, Richard J.

    2015-01-01

    The opportunistic pathogen Candida albicans colonizes the oral cavity and gastrointestinal tract. Adherence to host cells, extracellular matrix and salivary glycoproteins that coat oral surfaces, including prostheses, is an important prerequisite for colonization. In addition, interactions of C. albicans with commensal oral streptococci are suggested to promote retention and persistence of fungal cells in mixed-species communities. The hyphal filament specific cell wall protein Als3, a member of the Als protein family, is a major determinant in C. albicans adherence. Here, we utilized site-specific in-frame deletions within Als3 expressed on the surface of heterologous Saccharomyces cerevisiae to determine regions involved in interactions of Als3 with Streptococcus gordonii. N-terminal region amino acid residue deletions Δ166–225, Δ218–285, Δ270–305 and Δ277–286 were each effective in inhibiting binding of Strep. gordonii to Als3. In addition, these deletions differentially affected biofilm formation, hydrophobicity, and adherence to silicone and human tissue proteins. Deletion of the central repeat domain (Δ434–830) did not significantly affect interaction of Als3 with Strep. gordonii SspB protein, but affected other adherence properties and biofilm formation. Deletion of the amyloid-forming region (Δ325–331) did not affect interaction of Als3 with Strep. gordonii SspB adhesin, suggesting this interaction was amyloid-independent. These findings highlighted the essential function of the N-terminal domain of Als3 in mediating the interaction of C. albicans with S. gordonii, and suggested that amyloid formation is not essential for the inter-kingdom interaction. PMID:25332379

  8. Functional regions of Candida albicans hyphal cell wall protein Als3 that determine interaction with the oral bacterium Streptococcus gordonii.

    PubMed

    Bamford, Caroline V; Nobbs, Angela H; Barbour, Michele E; Lamont, Richard J; Jenkinson, Howard F

    2015-01-01

    The opportunistic pathogen Candida albicans colonizes the oral cavity and gastrointestinal tract. Adherence to host cells, extracellular matrix and salivary glycoproteins that coat oral surfaces, including prostheses, is an important prerequisite for colonization. In addition, interactions of C. albicans with commensal oral streptococci are suggested to promote retention and persistence of fungal cells in mixed-species communities. The hyphal filament specific cell wall protein Als3, a member of the Als protein family, is a major determinant in C. albicans adherence. Here, we utilized site-specific in-frame deletions within Als3 expressed on the surface of heterologous Saccharomyces cerevisiae to determine regions involved in interactions of Als3 with Streptococcus gordonii. N-terminal region amino acid residue deletions Δ166-225, Δ218-285, Δ270-305 and Δ277-286 were each effective in inhibiting binding of Strep. gordonii to Als3. In addition, these deletions differentially affected biofilm formation, hydrophobicity, and adherence to silicone and human tissue proteins. Deletion of the central repeat domain (Δ434-830) did not significantly affect interaction of Als3 with Strep. gordonii SspB protein, but affected other adherence properties and biofilm formation. Deletion of the amyloid-forming region (Δ325-331) did not affect interaction of Als3 with Strep. gordonii SspB adhesin, suggesting this interaction was amyloid-independent. These findings highlighted the essential function of the N-terminal domain of Als3 in mediating the interaction of C. albicans with S. gordonii, and suggested that amyloid formation is not essential for the inter-kingdom interaction.

  9. 7 CFR 611.21 - Availability of aerial photography.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 6 2011-01-01 2011-01-01 false Availability of aerial photography. 611.21 Section 611... § 611.21 Availability of aerial photography. The National Cartography and Geospatial Center obtains necessary clearance for all aerial photography for NRCS. New aerial photography of designated areas in...

  10. 7 CFR 611.21 - Availability of aerial photography.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Availability of aerial photography. 611.21 Section 611... § 611.21 Availability of aerial photography. The National Cartography and Geospatial Center obtains necessary clearance for all aerial photography for NRCS. New aerial photography of designated areas in...

  11. 7 CFR 611.21 - Availability of aerial photography.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 6 2012-01-01 2012-01-01 false Availability of aerial photography. 611.21 Section 611... § 611.21 Availability of aerial photography. The National Cartography and Geospatial Center obtains necessary clearance for all aerial photography for NRCS. New aerial photography of designated areas in...

  12. 7 CFR 611.21 - Availability of aerial photography.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 6 2013-01-01 2013-01-01 false Availability of aerial photography. 611.21 Section 611... § 611.21 Availability of aerial photography. The National Cartography and Geospatial Center obtains necessary clearance for all aerial photography for NRCS. New aerial photography of designated areas in...

  13. 7 CFR 611.21 - Availability of aerial photography.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 6 2014-01-01 2014-01-01 false Availability of aerial photography. 611.21 Section 611... § 611.21 Availability of aerial photography. The National Cartography and Geospatial Center obtains necessary clearance for all aerial photography for NRCS. New aerial photography of designated areas in...

  14. Dynamics of Actin Cables in Polarized Growth of the Filamentous Fungus Aspergillus nidulans

    PubMed Central

    Bergs, Anna; Ishitsuka, Yuji; Evangelinos, Minoas; Nienhaus, G. U.; Takeshita, Norio

    2016-01-01

    Highly polarized growth of filamentous fungi requires a continuous supply of proteins and lipids to the hyphal tip. This transport is managed by vesicle trafficking via the actin and microtubule cytoskeletons and their associated motor proteins. Particularly, actin cables originating from the hyphal tip are essential for hyphal growth. Although, specific marker proteins have been developed to visualize actin cables in filamentous fungi, the exact organization and dynamics of actin cables has remained elusive. Here, we observed actin cables using tropomyosin (TpmA) and Lifeact fused to fluorescent proteins in living Aspergillus nidulans hyphae and studied the dynamics and regulation. GFP tagged TpmA visualized dynamic actin cables formed from the hyphal tip with cycles of elongation and shrinkage. The elongation and shrinkage rates of actin cables were similar and approximately 0.6 μm/s. Comparison of actin markers revealed that high concentrations of Lifeact reduced actin dynamics. Simultaneous visualization of actin cables and microtubules suggests temporally and spatially coordinated polymerization and depolymerization between the two cytoskeletons. Our results provide new insights into the molecular mechanism of ordered polarized growth regulated by actin cables and microtubules. PMID:27242709

  15. Verification of Potency of Aerial Digital Oblique Cameras for Aerial Photogrammetry in Japan

    NASA Astrophysics Data System (ADS)

    Nakada, Ryuji; Takigawa, Masanori; Ohga, Tomowo; Fujii, Noritsuna

    2016-06-01

    Digital oblique aerial camera (hereinafter called "oblique cameras") is an assembly of medium format digital cameras capable of shooting digital aerial photographs in five directions i.e. nadir view and oblique views (forward and backward, left and right views) simultaneously and it is used for shooting digital aerial photographs efficiently for generating 3D models in a wide area. For aerial photogrammetry of public survey in Japan, it is required to use large format cameras, like DMC and UltraCam series, to ensure aerial photogrammetric accuracy. Although oblique cameras are intended to generate 3D models, digital aerial photographs in 5 directions taken with them should not be limited to 3D model production but they may also be allowed for digital mapping and photomaps of required public survey accuracy in Japan. In order to verify the potency of using oblique cameras for aerial photogrammetry (simultaneous adjustment, digital mapping and photomaps), (1) a viewer was developed to interpret digital aerial photographs taken with oblique cameras, (2) digital aerial photographs were shot with an oblique camera owned by us, a Penta DigiCAM of IGI mbH, and (3) accuracy of 3D measurements was verified.

  16. Locating buildings in aerial photos

    NASA Technical Reports Server (NTRS)

    Green, James S.

    1994-01-01

    Algorithms and techniques for use in the identification and location of large buildings in digitized copies of aerial photographs are developed and tested. The building data would be used in the simulation of objects located in the vicinity of an airport that may be detected by aircraft radar. Two distinct approaches are considered. Most building footprints are rectangular in form. The first approach studied is to search for right-angled corners that characterize rectangular objects and then to connect these corners to complete the building. This problem is difficult because many nonbuilding objects, such as street corners, parking lots, and ballparks often have well defined corners which are often difficult to distinguish from rooftops. Furthermore, rooftops come in a number of shapes, sizes, shadings, and textures which also limit the discrimination task. The strategy used linear sequences of different samples to detect straight edge segments at multiple angles and to determine when these segments meet at approximately right-angles with respect to each other. This technique is effective in locating corners. The test image used has a fairly rectangular block pattern oriented about thirty degrees clockwise from a vertical alignment, and the overall measurement data reflect this. However, this technique does not discriminate between buildings and other objects at an operationally suitable rate. In addition, since multiple paths are tested for each image pixel, this is a time consuming task. The process can be speeded up by preprocessing the image to locate the more optimal sampling paths. The second approach is to rely on a human operator to identify and select the building objects and then to have the computer determine the outline and location of the selected structures. When presented with a copy of a digitized aerial photograph, the operator uses a mouse and cursor to select a target building. After a button on the mouse is pressed, with the cursor fully within

  17. Draper Laboratory small autonomous aerial vehicle

    NASA Astrophysics Data System (ADS)

    DeBitetto, Paul A.; Johnson, Eric N.; Bosse, Michael C.; Trott, Christian A.

    1997-06-01

    The Charles Stark Draper Laboratory, Inc. and students from Massachusetts Institute of Technology and Boston University have cooperated to develop an autonomous aerial vehicle that won the 1996 International Aerial Robotics Competition. This paper describes the approach, system architecture and subsystem designs for the entry. This entry represents a combination of many technology areas: navigation, guidance, control, vision processing, human factors, packaging, power, real-time software, and others. The aerial vehicle, an autonomous helicopter, performs navigation and control functions using multiple sensors: differential GPS, inertial measurement unit, sonar altimeter, and a flux compass. The aerial transmits video imagery to the ground. A ground based vision processor converts the image data into target position and classification estimates. The system was designed, built, and flown in less than one year and has provided many lessons about autonomous vehicle systems, several of which are discussed. In an appendix, our current research in augmenting the navigation system with vision- based estimates is presented.

  18. Reliable aerial thermography for energy conservation

    NASA Technical Reports Server (NTRS)

    Jack, J. R.; Bowman, R. L.

    1981-01-01

    A method for energy conservation, the aerial thermography survey, is discussed. It locates sources of energy losses and wasteful energy management practices. An operational map is presented for clear sky conditions. The map outlines the key environmental conditions conductive to obtaining reliable aerial thermography. The map is developed from defined visual and heat loss discrimination criteria which are quantized based on flat roof heat transfer calculations.

  19. Modulation of Phagosomal pH by Candida albicans Promotes Hyphal Morphogenesis and Requires Stp2p, a Regulator of Amino Acid Transport

    PubMed Central

    Vylkova, Slavena; Lorenz, Michael C.

    2014-01-01

    Candida albicans, the most important fungal pathogen of humans, has a unique interaction with macrophages in which phagocytosis induces a switch from the yeast to hyphal form, allowing it to escape by rupturing the immune cell. While a variety of factors induce this switch in vitro, including neutral pH, it is not clear what triggers morphogenesis within the macrophage where the acidic environment should inhibit this transition. In vitro, C. albicans grown in similar conditions in which amino acids are the primary carbon source generate large quantities of ammonia to raise the extracellular pH and induce the hyphal switch. We show here that C. albicans cells neutralize the macrophage phagosome and that neutral pH is a key inducer of germination in phagocytosed cells by using a mutant lacking STP2, a transcription factor that regulates the expression of multiple amino acid permeases, that is completely deficient in alkalinization in vitro. Phagocytosed stp2Δ mutant cells showed significant reduction in hypha formation and escaped from macrophages less readily compared to wild type cells; as a result stp2Δ mutant cells were killed at a higher rate and caused less damage to RAW264.7 macrophages. Stp2p-regulated import leads to alkalinization of the phagosome, since the majority of the wild type cells fail to co-localize with acidophilic dyes, whereas the stp2Δ mutant cells were located in acidic phagosomes. Furthermore, stp2Δ mutant cells were able to form hyphae and escape from neutral phagosomes, indicating that the survival defect in these cells was pH dependent. Finally, these defects are reflected in an attenuation of virulence in a mouse model of disseminated candidiasis. Altogether our results suggest that C. albicans utilizes amino acids to promote neutralization of the phagosomal pH, hyphal morphogenesis, and escape from macrophages. PMID:24626429

  20. Growth

    NASA Astrophysics Data System (ADS)

    Waag, Andreas

    This chapter is devoted to the growth of ZnO. It starts with various techniques to grow bulk samples and presents in some detail the growth of epitaxial layers by metal organic chemical vapor deposition (MOCVD), molecular beam epitaxy (MBE), and pulsed laser deposition (PLD). The last section is devoted to the growth of nanorods. Some properties of the resulting samples are also presented. If a comparison between GaN and ZnO is made, very often the huge variety of different growth techniques available to fabricate ZnO is said to be an advantage of this material system. Indeed, growth techniques range from low cost wet chemical growth at almost room temperature to high quality MOCVD growth at temperatures above 1, 000∘C. In most cases, there is a very strong tendency of c-axis oriented growth, with a much higher growth rate in c-direction as compared to other crystal directions. This often leads to columnar structures, even at relatively low temperatures. However, it is, in general, not straight forward to fabricate smooth ZnO thin films with flat surfaces. Another advantage of a potential ZnO technology is said to be the possibility to grow thin films homoepitaxially on ZnO substrates. ZnO substrates are mostly fabricated by vapor phase transport (VPT) or hydrothermal growth. These techniques are enabling high volume manufacturing at reasonable cost, at least in principle. The availability of homoepitaxial substrates should be beneficial to the development of ZnO technology and devices and is in contrast to the situation of GaN. However, even though a number of companies are developing ZnO substrates, only recently good quality substrates have been demonstrated. However, these substrates are not yet widely available. Still, the situation concerning ZnO substrates seems to be far from low-cost, high-volume production. The fabrication of dense, single crystal thin films is, in general, surprisingly difficult, even when ZnO is grown on a ZnO substrate. However

  1. UDP-glucose pyrophosphorylase influences polysaccharide synthesis, cell wall components, and hyphal branching in Ganoderma lucidum via regulation of the balance between glucose-1-phosphate and UDP-glucose.

    PubMed

    Li, Mengjiao; Chen, Tianxi; Gao, Tan; Miao, Zhigang; Jiang, Ailiang; Shi, Liang; Ren, Ang; Zhao, Mingwen

    2015-09-01

    UDP-glucose pyrophosphorylase (UGP) is a key enzyme involved in carbohydrate metabolism, but there are few studies on the functions of this enzyme in fungi. The ugp gene of Ganoderma lucidum was cloned, and enzyme kinetic parameters of the UGP recombinant protein were determined in vitro, revealing that this protein was functional and catalyzed the reversible conversion between Glc-1-P and UDP-Glc. ugp silencing by RNA interference resulted in changes in the levels of the intermediate metabolites Glc-1-P and UDP-Glc. The compounds and structure of the cell wall in the silenced strains were also altered compared with those in the wild-type strains. Moreover, the number of hyphal branches was also changed in the silenced strains. To verify the role of UGP in hyphal branching, a ugp-overexpressing strain was constructed. The results showed that the number of hyphal branches was influenced by UGP. The mechanism underlying hyphal branching was further investigated by adding exogenous Glc-1-P. Our results showed that hyphal branching was regulated by a change in the cytosolic Ca(2+) concentration, which was affected by the level of the intermediate metabolite Glc-1-P, in G. lucidum. Our findings indicate the existence of an interaction between carbon metabolism and Ca(2+) signaling in this fungus.

  2. Aerial photo SBVC1962". Photo no. 360. Low oblique aerial view ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Aerial photo -SBVC-1962". Photo no. 360. Low oblique aerial view of the campus, looking southeast. Stamped on the rear: "Ron Wilhite, Sun-Telegram photo, file, 10/22/62/ - San Bernardino Valley College, 701 South Mount Vernon Avenue, San Bernardino, San Bernardino County, CA

  3. Enrichment and Broad Representation of Plant Biomass-Degrading Enzymes in the Specialized Hyphal Swellings of Leucoagaricus gongylophorus, the Fungal Symbiont of Leaf-Cutter Ants

    SciTech Connect

    Aylward, Frank O.; Khadempour, Lily; Tremmel, Daniel; McDonald, Bradon R.; Nicora, Carrie D.; Wu, Si; Moore, Ronald J.; Orton, Daniel J.; Monroe, Matthew E.; Piehowski, Paul D.; Purvine, Samuel O.; Smith, Richard D.; Lipton, Mary S.; Burnum-Johnson, Kristin E.; Currie, Cameron R.

    2015-08-28

    Leaf-cutter ants are prolific and conspicuous Neotropical herbivores that derive energy from specialized fungus gardens they cultivate using foliar biomass. The basidiomycetous cultivar of the ants, Leucoagaricus gongylophorus, produces specialized hyphal swellings called gongylidia that serve as the primary food source of ant colonies. Gongylidia also contain lignocellulases that become concentrated in ant digestive tracts and are deposited within fecal droplets onto fresh foliar material as it is foraged by the ants. Although the enzymes concentrated by L. gongylophorus within gongylidia are thought to be critical to the initial degradation of plant biomass, only a few enzymes present in these hyphal swellings have been identified. Here we use proteomic methods to identify proteins present in the gongylidia of three Atta cephalotes colonies. Our results demonstrate that a diverse but consistent set of enzymes is present in gongylidia, including numerous lignocellulases likely involved in the degradation of polysaccharides, plant toxins, and proteins. Overall, gongylidia contained over three-quarters of all lignocellulases identified in the L. gongylophorus genome, demonstrating that the majority of the enzymes produced by this fungus for biomass breakdown are ingested by the ants. We also identify a set of 23 lignocellulases enriched in gongylidia compared to whole fungus garden samples, suggesting that certain enzymes may be particularly important in the initial degradation of foliar material. Our work sheds light on the complex interplay between leaf-cutter ants and their fungal symbiont that allows for the host insects to occupy an herbivorous niche by indirectly deriving energy from plant biomass.

  4. A potential phosphorylation site for an A-type kinase in the Efg1 regulator protein contributes to hyphal morphogenesis of Candida albicans.

    PubMed Central

    Bockmühl, D P; Ernst, J F

    2001-01-01

    Efg1p in the human fungal pathogen Candida albicans is a member of the conserved APSES class of proteins regulating morphogenetic processes in fungi. We have analyzed the importance for hyphal morphogenesis of a putative phosphorylation site for protein kinase A (PKA), threonine-206, within an Efg1p domain highly conserved among APSES proteins. Alanine substitution of T206, but not of the adjacent T207 and T208 residues, led to a block of hypha formation on solid and in liquid media, while a T206E exchange caused hyperfilamentation. The extent of the morphogenetic defect caused by the T206A mutation depended on hypha-induction conditions. Extragenous suppression of mutations in signaling components, including tpk2 and cek1 mutations, was achieved by wild-type- and T206E-, but not by the T206A-variant-encoding allele of EFG1. All muteins tested were produced at equal levels and at high production levels supported pseudohyphal formation. The results are consistent with a role of Efg1p as a central downstream component of a PKA-signaling pathway including Tpk2p or other PKA isoforms. Threonine-206 of Efg1p is essential as a putative phosphorylation target to promote hyphal induction by a subset of environmental cues. PMID:11290709

  5. Aerial radiation survey at a military range.

    SciTech Connect

    Williams, G. P.; Martino, L. E.; Wrobel, J.; Environmental Assessment; U.S. Army Aberdeen Proving Ground

    2001-04-01

    Aberdeen Proving Ground (APG) is currently listed on the Superfund National Priorities List because of past waste handling practices at 13 'study areas.' Concern has been expressed that anthropogenic radioisotopes may have been released at some of the study areas, with the potential of posing health risks to human or ecological receptors. This concern was addressed by thoroughly searching archival records, sampling and analyzing environmental media, and performing an aerial radiation survey. The aerial radiation survey techniques employed have been used over all U.S. Department of Energy and commercial reactor sites. Use of the Aerial Measurement System (AMS) allowed investigators to safely survey areas where surveys using hand-held instruments would be difficult to perform. In addition, the AMS delivered a full spectrum of the measured gamma radiation, thereby providing a means of determining which radioisotopes were present at the surface. As a quality check on the aerial measurements, four ground truth measurements were made at selected locations and compared with the aerial data for the same locations. The results of the survey revealed no evidence of surface radioactive contamination. The measured background radiation, including the cosmic contribution, ranged from 4 to 11 {mu}R/h.

  6. Looking for an old aerial photograph

    USGS Publications Warehouse

    ,

    1997-01-01

    Attempts to photograph the surface of the Earth date from the 1800's, when photographers attached cameras to balloons, kites, and even pigeons. Today, aerial photographs and satellite images are commonplace. The rate of acquiring aerial photographs and satellite images has increased rapidly in recent years. Views of the Earth obtained from aircraft or satellites have become valuable tools to Government resource planners and managers, land-use experts, environmentalists, engineers, scientists, and a wide variety of other users. Many people want historical aerial photographs for business or personal reasons. They may want to locate the boundaries of an old farm or a piece of family property. Or they may want a photograph as a record of changes in their neighborhood, or as a gift. The U.S. Geological Survey (USGS) maintains the Earth Science Information Centers (ESIC?s) to sell aerial photographs, remotely sensed images from satellites, a wide array of digital geographic and cartographic data, as well as the Bureau?s wellknown maps. Declassified photographs from early spy satellites were recently added to the ESIC offerings of historical images. Using the Aerial Photography Summary Record System database, ESIC researchers can help customers find imagery in the collections of other Federal agencies and, in some cases, those of private companies that specialize in esoteric products.

  7. Aerial Measuring System Sensor Modeling

    SciTech Connect

    R. S. Detwiler

    2002-04-01

    This project deals with the modeling the Aerial Measuring System (AMS) fixed-wing and rotary-wing sensor systems, which are critical U.S. Department of Energy's National Nuclear Security Administration (NNSA) Consequence Management assets. The fixed-wing system is critical in detecting lost or stolen radiography or medical sources, or mixed fission products as from a commercial power plant release at high flying altitudes. The helicopter is typically used at lower altitudes to determine ground contamination, such as in measuring americium from a plutonium ground dispersal during a cleanup. Since the sensitivity of these instruments as a function of altitude is crucial in estimating detection limits of various ground contaminations and necessary count times, a characterization of their sensitivity as a function of altitude and energy is needed. Experimental data at altitude as well as laboratory benchmarks is important to insure that the strong effects of air attenuation are modeled correctly. The modeling presented here is the first attempt at such a characterization of the equipment for flying altitudes. The sodium iodide (NaI) sensors utilized with these systems were characterized using the Monte Carlo N-Particle code (MCNP) developed at Los Alamos National Laboratory. For the fixed wing system, calculations modeled the spectral response for the 3-element NaI detector pod and High-Purity Germanium (HPGe) detector, in the relevant energy range of 50 keV to 3 MeV. NaI detector responses were simulated for both point and distributed surface sources as a function of gamma energy and flying altitude. For point sources, photopeak efficiencies were calculated for a zero radial distance and an offset equal to the altitude. For distributed sources approximating an infinite plane, gross count efficiencies were calculated and normalized to a uniform surface deposition of 1 {micro}Ci/m{sup 2}. The helicopter calculations modeled the transport of americium-241 ({sup 241}Am

  8. Shutter/aperture settings for aerial photography

    NASA Technical Reports Server (NTRS)

    Lockwood, H. E.; Perry, L.

    1976-01-01

    Determination of aerial camera shutter and aperture settings to produce consistently high-quality aerial photographs is a task complicated by numerous variables. Presented in this article are brief discussions of each variable and specific data which may be used for the systematic control of each. The variables discussed include sunlight, aircraft altitude, subject and season, film speed, and optical system. Data which may be used as a base reference are included, and encompass two sets of sensitometric specifications for two film-chemistry processes along with camera-aircraft parameters, which have been established and used to produce good exposures. Information contained here may be used to design and implement an exposure-determination system for aerial photography.

  9. USGS Releases New Digital Aerial Products

    USGS Publications Warehouse

    ,

    2005-01-01

    The U.S. Geological Survey (USGS) Center for Earth Resources Observation and Science (EROS) has initiated distribution of digital aerial photographic products produced by scanning or digitizing film from its historical aerial photography film archive. This archive, located in Sioux Falls, South Dakota, contains thousands of rolls of film that contain more than 8 million frames of historic aerial photographs. The largest portion of this archive consists of original film acquired by Federal agencies from the 1930s through the 1970s to produce 1:24,000-scale USGS topographic quadrangle maps. Most of this photography is reasonably large scale (USGS photography ranges from 1:8,000 to 1:80,000) to support the production of the maps. Two digital products are currently available for ordering: high-resolution scanned products and medium-resolution digitized products.

  10. The Development and Flight Testing of an Aerially Deployed Unmanned Aerial System

    NASA Astrophysics Data System (ADS)

    Smith, Andrew

    An investigation into the feasibility of aerial deployed unmanned aerial vehicles was completed. The investigation included the development and flight testing of multiple unmanned aerial systems to investigate the different components of potential aerial deployment missions. The project consisted of two main objectives; the first objective dealt with the development of an airframe capable of surviving aerial deployment from a rocket and then self assembling from its stowed configuration into its flight configuration. The second objective focused on the development of an autopilot capable of performing basic guidance, navigation, and control following aerial deployment. To accomplish these two objectives multiple airframes were developed to verify their completion experimentally. The first portion of the project, investigating the feasibility of surviving an aerial deployment, was completed using a fixed wing glider that following a successful deployment had 52 seconds of controlled flight. Before developing the autopilot in the second phase of the project, the glider was significantly upgraded to fix faults discovered in the glider flight testing and to enhance the system capabilities. Unfortunately to conform to outdoor flight restrictions imposed by the university and the Federal Aviation Administration it was required to switch airframes before flight testing of the new fixed wing platform could begin. As a result, an autopilot was developed for a quadrotor and verified experimentally completely indoors to remain within the limits of governing policies.

  11. MicroProbe Small Unmanned Aerial System

    NASA Technical Reports Server (NTRS)

    Bland, Geoffrey; Miles, Ted

    2012-01-01

    The MicroProbe unmanned aerial system (UAS) concept incorporates twin electric motors mounted on the vehicle wing, thus enabling an aerodynamically and environmentally clean nose area for atmospheric sensors. A payload bay is also incorporated in the fuselage to accommodate remote sensing instruments. A key feature of this concept is lightweight construction combined with low flying speeds to minimize kinetic energy and associated hazards, as well as maximizing spatial resolution. This type of aerial platform is needed for Earth science research and environmental monitoring. There were no vehicles of this type known to exist previously.

  12. Metrically preserving the USGS aerial film archive

    USGS Publications Warehouse

    Moe, Donald; Longhenry, Ryan

    2013-01-01

    Since 1972, the U.S. Geological Survey (USGS) Earth Resources Observation and Science (EROS) Center in Sioux Falls, South Dakota, has provided fi lm-based products to the public. EROS is home to an archive of 12 million frames of analog photography ranging from 1937 to the present. The archive contains collections from both aerial and satellite platforms including programs such as the National High Altitude Program (NHAP), National Aerial Photography Program (NAPP), U.S. Antarctic Resource Center (USARC), Declass 1(CORONA, ARGON, and LANYARD), Declass 2 (KH-7 and KH-9), and Landsat (1972 – 1992, Landsat 1–5).

  13. Advanced Image Processing of Aerial Imagery

    NASA Technical Reports Server (NTRS)

    Woodell, Glenn; Jobson, Daniel J.; Rahman, Zia-ur; Hines, Glenn

    2006-01-01

    Aerial imagery of the Earth is an invaluable tool for the assessment of ground features, especially during times of disaster. Researchers at the NASA Langley Research Center have developed techniques which have proven to be useful for such imagery. Aerial imagery from various sources, including Langley's Boeing 757 Aries aircraft, has been studied extensively. This paper discusses these studies and demonstrates that better-than-observer imagery can be obtained even when visibility is severely compromised. A real-time, multi-spectral experimental system will be described and numerous examples will be shown.

  14. Ground cover estimated from aerial photographs

    NASA Technical Reports Server (NTRS)

    Gerbermann, A. H.; Cuellar, J. A.; Wiegand, C. L.

    1976-01-01

    Estimates of per cent ground cover made by ground observers were compared with independent estimates made on the basis of low-altitude (640-1219 m) aerial photographs of the same fields. Standard statistical simple correlation and linear regression analyses revealed a high correlation between the two estimation methods. In crops such as grain, sorghum, corn, and forage sorghum, in which the broadest part of the leaf canopy is near the top of the plant, there was a tendency to overestimate the per cent ground cover from aerial photographs.

  15. Noise from aerial bursts of fireworks

    NASA Technical Reports Server (NTRS)

    Maglieri, D. J.; Henderson, H. R.

    1973-01-01

    A study was made recording the pressure time histories of the aerial bursts of mortars of various sizes launched during an actual fireworks display. The peak overpressure and duration of blast noise as well as the energy spectral density are compared with the characteristics of a blasting cap and of an F-104 aircraft at a Mach number of 1.4 and an altitude of 42,000 ft. Noise levels of the fireworks aerial bursts peaked 15 decibels below levels deemed damaging to hearing.

  16. Laser Doppler velocimeter aerial spray measurements

    NASA Technical Reports Server (NTRS)

    Zalay, A. D.; Eberle, W. R.; Howle, R. E.; Shrider, K. R.

    1978-01-01

    An experimental research program for measuring the location, spatial extent, and relative concentration of airborne spray clouds generated by agricultural aircraft is described. The measurements were conducted with a ground-based laser Doppler velocimeter. The remote sensing instrumentation, experimental tests, and the results of the flight tests are discussed. The cross section of the aerial spray cloud and the observed location, extent, and relative concentration of the airborne particulates are presented. It is feasible to use a mobile laser Doppler velocimeter to track and monitor the transport and dispersion of aerial spray generated by an agricultural aircraft.

  17. AERIAL OF VISITORS INFORMATION CENTER [VIC] & ROCKET GARDEN

    NASA Technical Reports Server (NTRS)

    1973-01-01

    AERIAL OF VISITORS INFORMATION CENTER [VIC] & ROCKET GARDEN KSC-373C-0556.20 116-KSC-373C-556.20, P-01622-B, ARCHIVE-04455 Aerial view of Easter crowds at Visitors Information Center, Kennedy Space Center, Florida.

  18. Loss of growth polarity and mislocalization of septa in a Neurospora mutant altered in the regulatory subunit of cAMP-dependent protein kinase.

    PubMed Central

    Bruno, K S; Aramayo, R; Minke, P F; Metzenberg, R L; Plamann, M

    1996-01-01

    In filamentous fungi, growth polarity (i.e. hyphal extension) and formation of septa require polarized deposition of new cell wall material. To explore this process, we analyzed a conditional Neurospora crassa mutant, mcb, which showed a complete loss of growth polarity when incubated at the restrictive temperature. Cloning and DNA sequence analysis of the mcb gene revealed that it encodes a regulatory subunit of cAMP-dependent protein kinase (PKA). Unexpectedly, the mcb mutant still formed septa when grown at the restrictive temperature, indicating that polarized deposition of wall material during septation is a process that is, at least in part, independent of polarized deposition during hyphal tip extension. However, septa formed in the mcb mutant growing at the restrictive temperature are mislocalized. Both polarized growth and septation are actin-dependent processes, and a concentration of actin patches is observed at growing hyphal tips and sites where septa are being formed. In the mcb mutant growing at the restrictive temperature, actin patches are uniformly distributed over the cell cortex; however, actin patches are still concentrated at sites of septation. Our results suggest that the PKA pathway regulates hyphal growth polarity, possibly through organizing actin patches at the cell cortex. Images PMID:8918454

  19. 77 FR 36250 - Information Collection Request; Request for Aerial Photography

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-18

    ...; ] DEPARTMENT OF AGRICULTURE Farm Service Agency Information Collection Request; Request for Aerial Photography... FSA Aerial Photography Program. The FSA Aerial Photography Field Office (APFO) uses the information from this form to collect the customer and photography information needed to produce and ship...

  20. 47 CFR 32.6421 - Aerial cable expense.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false Aerial cable expense. 32.6421 Section 32.6421... FOR TELECOMMUNICATIONS COMPANIES Instructions for Expense Accounts § 32.6421 Aerial cable expense. (a) This account shall include expenses associated with aerial cable. (b) Subsidiary record...

  1. Geography via Aerial Field Trips: Do It This Way, 6.

    ERIC Educational Resources Information Center

    Richason, Benjamin F., Jr.; Guell, Carl E.

    To provide guidance for geography teachers, this booklet presents information on how to plan and execute aerial field trips. The aerial field trip can be employed as an effective visual aid technique in the teaching of geography, especially for presenting earth generalizations and interrelationships. The benefits of an aerial field trip are…

  2. "A" Is for Aerial Maps and Art

    ERIC Educational Resources Information Center

    Todd, Reese H.; Delahunty, Tina

    2007-01-01

    The technology of satellite imagery and remote sensing adds a new dimension to teaching and learning about maps with elementary school children. Just a click of the mouse brings into view some images of the world that could only be imagined a generation ago. Close-up aerial pictures of the school and neighborhood quickly catch the interest of…

  3. 47 CFR 32.2421 - Aerial cable.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... cost of optical fiber cable and other associated material used in constructing a physical path for the... cable or aerial wire as well as the cost of other material used in construction of such plant... the original cost of single or paired conductor cable, wire and other associated material used...

  4. Sea Ice Mapping using Unmanned Aerial Systems

    NASA Astrophysics Data System (ADS)

    Solbø, S.; Storvold, R.

    2011-12-01

    Mapping of sea ice extent and sea ice features is an important task in climate research. Since the arctic coastal and oceanic areas have a high probability of cloud coverage, aerial platforms are superior to satellite measurements for high-resolution optical measurements. However, routine observations of sea ice conditions present a variety of problems using conventional piloted aircrafts. Specially, the availability of suitable aircrafts for lease does not cover the demand in major parts of the arctic. With the recent advances in unmanned aerial systems (UAS), there is a high possibility of establishing routine, cost effective aerial observations of sea ice conditions in the near future. Unmanned aerial systems can carry a wide variety of sensors useful for characterizing sea-ice features. For instance, the CryoWing UAS, a system initially designed for measurements of the cryosphere, can be equipped with digital cameras, surface thermometers and laser altimeters for measuring freeboard of ice flows. In this work we will present results from recent CryoWing sea ice flights on Svalbard, Norway. The emphasis will be on data processing for stitching together images acquired with the non-stabilized camera payload, to form high-resolution mosaics covering large spatial areas. These data are being employed to map ice conditions; including ice and lead features and melt ponds. These high-resolution mosaics are also well suited for sea-ice mechanics, classification studies and for validation of satellite sea-ice products.

  5. Calculating aerial images from EUV masks

    NASA Astrophysics Data System (ADS)

    Pistor, Thomas V.; Neureuther, Andrew R.

    1999-06-01

    Aerial images for line/space patterns, arrays of posts and an arbitrary layout pattern are calculated for EUV masks in a 4X EUV imaging system. Both mask parameters and illumination parameters are varied to investigate their effects on the aerial image. To facilitate this study, a parallel version of TEMPEST with a Fourier transform boundary condition was developed and run on a network of 24 microprocessors. Line width variations are observed when absorber thickness or sidewall angle changes. As the line/space pattern scales to smaller dimensions, the aspect ratios of the absorber features increase, introducing geometric shadowing and reducing aerial image intensity and contrast. 100nm square posts have circular images of diameter close to 100nm, but decreasing in diameter significantly when the corner round radius at the mask becomes greater than 50 nm. Exterior mask posts image slightly smaller and with higher ellipticity than interior mask posts. The aerial image of the arbitrary test pattern gives insight into the effects of the off-axis incidence employed in EUV lithography systems.

  6. A TOOL FOR PLANNING AERIAL PHOTOGRAPHY

    EPA Science Inventory

    abstract The U.S. EPAs Pacific Coastal Ecology Branch has developed a tool in the form of an Excel. spreadsheet that facilitates planning aerial photography missions. The spreadsheet accepts various input parameters such as desired photo-scale and boundary coordinates of the stud...

  7. Aerial Scene Recognition using Efficient Sparse Representation

    SciTech Connect

    Cheriyadat, Anil M

    2012-01-01

    Advanced scene recognition systems for processing large volumes of high-resolution aerial image data are in great demand today. However, automated scene recognition remains a challenging problem. Efficient encoding and representation of spatial and structural patterns in the imagery are key in developing automated scene recognition algorithms. We describe an image representation approach that uses simple and computationally efficient sparse code computation to generate accurate features capable of producing excellent classification performance using linear SVM kernels. Our method exploits unlabeled low-level image feature measurements to learn a set of basis vectors. We project the low-level features onto the basis vectors and use simple soft threshold activation function to derive the sparse features. The proposed technique generates sparse features at a significantly lower computational cost than other methods~\\cite{Yang10, newsam11}, yet it produces comparable or better classification accuracy. We apply our technique to high-resolution aerial image datasets to quantify the aerial scene classification performance. We demonstrate that the dense feature extraction and representation methods are highly effective for automatic large-facility detection on wide area high-resolution aerial imagery.

  8. 29 CFR 1926.453 - Aerial lifts.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... ground: (i) Extensible boom platforms; (ii) Aerial ladders; (iii) Articulating boom platforms; (iv... articulating boom platforms. (i) Lift controls shall be tested each day prior to use to determine that such... when outriggers are used, they shall be positioned on pads or a solid surface. Wheel chocks shall...

  9. 29 CFR 1926.453 - Aerial lifts.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... ground: (i) Extensible boom platforms; (ii) Aerial ladders; (iii) Articulating boom platforms; (iv... articulating boom platforms. (i) Lift controls shall be tested each day prior to use to determine that such... when outriggers are used, they shall be positioned on pads or a solid surface. Wheel chocks shall...

  10. Aerial Infrared Photos for Citrus Growers

    NASA Technical Reports Server (NTRS)

    Blazquez, C. H.; Horn, F. W. J.

    1982-01-01

    Handbook advises on benefits and methods of aerial photography with color infrared film. Interpretation of photographs is discussed in detail. Necessary equipment for interpretation is described--light table, magnifying lenses, and microfiche viewers, for example. Advice is given on rating tree condition; identifying effects of diseases, insects, and nematodes; and evaluating effects of soil, water, and weather.

  11. 47 CFR 32.2431 - Aerial wire.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 47 Telecommunication 2 2013-10-01 2013-10-01 false Aerial wire. 32.2431 Section 32.2431 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES UNIFORM SYSTEM OF ACCOUNTS... construction of such plant. (b) The cost of permits and privileges for the construction of cable and...

  12. 47 CFR 32.2431 - Aerial wire.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 47 Telecommunication 2 2012-10-01 2012-10-01 false Aerial wire. 32.2431 Section 32.2431 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES UNIFORM SYSTEM OF ACCOUNTS... construction of such plant. (b) The cost of permits and privileges for the construction of cable and...

  13. 47 CFR 32.2431 - Aerial wire.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 47 Telecommunication 2 2014-10-01 2014-10-01 false Aerial wire. 32.2431 Section 32.2431 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES UNIFORM SYSTEM OF ACCOUNTS... construction of such plant. (b) The cost of permits and privileges for the construction of cable and...

  14. The Neurospora crassa exocyst complex tethers Spitzenkörper vesicles to the apical plasma membrane during polarized growth

    PubMed Central

    Riquelme, Meritxell; Bredeweg, Erin L.; Callejas-Negrete, Olga; Roberson, Robert W.; Ludwig, Sarah; Beltrán-Aguilar, Alejandro; Seiler, Stephan; Novick, Peter; Freitag, Michael

    2014-01-01

    Fungal hyphae are among the most highly polarized cells. Hyphal polarized growth is supported by tip-directed transport of secretory vesicles, which accumulate temporarily in a stratified manner in an apical vesicle cluster, the Spitzenkörper. The exocyst complex is required for tethering of secretory vesicles to the apical plasma membrane. We determined that the presence of an octameric exocyst complex is required for the formation of a functional Spitzenkörper and maintenance of regular hyphal growth in Neurospora crassa. Two distinct localization patterns of exocyst subunits at the hyphal tip suggest the dynamic formation of two assemblies. The EXO-70/EXO-84 subunits are found at the peripheral part of the Spitzenkörper, which partially coincides with the outer macrovesicular layer, whereas exocyst components SEC-5, -6, -8, and -15 form a delimited crescent at the apical plasma membrane. Localization of SEC-6 and EXO-70 to the plasma membrane and the Spitzenkörper, respectively, depends on actin and microtubule cytoskeletons. The apical region of exocyst-mediated vesicle fusion, elucidated by the plasma membrane–associated exocyst subunits, indicates the presence of an exocytotic gradient with a tip-high maximum that dissipates gradually toward the subapex, confirming the earlier predictions of the vesicle supply center model for hyphal morphogenesis. PMID:24523289

  15. Object and activity detection from aerial video

    NASA Astrophysics Data System (ADS)

    Se, Stephen; Shi, Feng; Liu, Xin; Ghazel, Mohsen

    2015-05-01

    Aerial video surveillance has advanced significantly in recent years, as inexpensive high-quality video cameras and airborne platforms are becoming more readily available. Video has become an indispensable part of military operations and is now becoming increasingly valuable in the civil and paramilitary sectors. Such surveillance capabilities are useful for battlefield intelligence and reconnaissance as well as monitoring major events, border control and critical infrastructure. However, monitoring this growing flood of video data requires significant effort from increasingly large numbers of video analysts. We have developed a suite of aerial video exploitation tools that can alleviate mundane monitoring from the analysts, by detecting and alerting objects and activities that require analysts' attention. These tools can be used for both tactical applications and post-mission analytics so that the video data can be exploited more efficiently and timely. A feature-based approach and a pixel-based approach have been developed for Video Moving Target Indicator (VMTI) to detect moving objects at real-time in aerial video. Such moving objects can then be classified by a person detector algorithm which was trained with representative aerial data. We have also developed an activity detection tool that can detect activities of interests in aerial video, such as person-vehicle interaction. We have implemented a flexible framework so that new processing modules can be added easily. The Graphical User Interface (GUI) allows the user to configure the processing pipeline at run-time to evaluate different algorithms and parameters. Promising experimental results have been obtained using these tools and an evaluation has been carried out to characterize their performance.

  16. Three-dimensional image analysis of plugging at the septal pore by Woronin body during hypotonic shock inducing hyphal tip bursting in the filamentous fungus Aspergillus oryzae.

    PubMed

    Maruyama, Jun-ichi; Juvvadi, Praveen Rao; Ishi, Kazutomo; Kitamoto, Katsuhiko

    2005-06-17

    We observed that the filamentous fungus, Aspergillus oryzae, grown on agar media burst out cytoplasmic constituents from the hyphal tip soon after flooding with water. Woronin body is a specialized organelle known to plug the septal pore adjacent to the lysed compartment to prevent extensive loss of cytoplasm. A. oryzae Aohex1 gene homologous to Neurospora crassa HEX1 gene encoding a major protein in Woronin body was expressed as a fusion with DsRed2, resulting in visualization of Woronin body. Confocal microscopy and three-dimensional reconstruction of images visualized the septal pore as a dark region surrounded by green fluorescence of EGFP-fused secretory protein, RNase T1, on the septum. Dual fluorescent labeling revealed the plugging of the septal pores adjacent to the lysed apical compartments by Woronin bodies during hypotonic shock. Disruption of Aohex1 gene caused disappearance of Woronin bodies and the defect to prevent extensive loss of cytoplasm during hypotonic shock. PMID:15882988

  17. Three-dimensional image analysis of plugging at the septal pore by Woronin body during hypotonic shock inducing hyphal tip bursting in the filamentous fungus Aspergillus oryzae

    SciTech Connect

    Maruyama, Jun-ichi; Juvvadi, Praveen Rao; Ishi, Kazutomo; Kitamoto, Katsuhiko . E-mail: akitamo@mail.ecc.u-tokyo.ac.jp

    2005-06-17

    We observed that the filamentous fungus, Aspergillus oryzae, grown on agar media burst out cytoplasmic constituents from the hyphal tip soon after flooding with water. Woronin body is a specialized organelle known to plug the septal pore adjacent to the lysed compartment to prevent extensive loss of cytoplasm. A. oryzae Aohex1 gene homologous to Neurospora crassa HEX1 gene encoding a major protein in Woronin body was expressed as a fusion with DsRed2, resulting in visualization of Woronin body. Confocal microscopy and three-dimensional reconstruction of images visualized the septal pore as a dark region surrounded by green fluorescence of EGFP-fused secretory protein, RNase T1, on the septum. Dual fluorescent labeling revealed the plugging of the septal pores adjacent to the lysed apical compartments by Woronin bodies during hypotonic shock. Disruption of Aohex1 gene caused disappearance of Woronin bodies and the defect to prevent extensive loss of cytoplasm during hypotonic shock.

  18. Environmental application of aerial reconnaissance to search for open dumps

    NASA Astrophysics Data System (ADS)

    Getz, Thomas J.; Randolph, J. C.; Echelberger, Wayne F.

    1983-11-01

    Three approaches to using aerial photography are evaluated for searching for open dumps in the state of Indiana. Photography with hand-held cameras from a small airplane proved more effective and flexible than either photo-interpretation of existing air photos or subcontracting to a federal agency for new aerial photography. The rationale for our choice of aerial reconnaissance, other uses of low-level aerial surveillance, the utility of small-format camera aerial photography for environmental analysis, and methods used for locating open dumps are discussed.

  19. Enrichment and Broad Representation of Plant Biomass-Degrading Enzymes in the Specialized Hyphal Swellings of Leucoagaricus gongylophorus, the Fungal Symbiont of Leaf-Cutter Ants.

    PubMed

    Aylward, Frank O; Khadempour, Lily; Tremmel, Daniel M; McDonald, Bradon R; Nicora, Carrie D; Wu, Si; Moore, Ronald J; Orton, Daniel J; Monroe, Matthew E; Piehowski, Paul D; Purvine, Samuel O; Smith, Richard D; Lipton, Mary S; Burnum-Johnson, Kristin E; Currie, Cameron R

    2015-01-01

    Leaf-cutter ants are prolific and conspicuous constituents of Neotropical ecosystems that derive energy from specialized fungus gardens they cultivate using prodigious amounts of foliar biomass. The basidiomycetous cultivar of the ants, Leucoagaricus gongylophorus, produces specialized hyphal swellings called gongylidia that serve as the primary food source of ant colonies. Gongylidia also contain plant biomass-degrading enzymes that become concentrated in ant digestive tracts and are deposited within fecal droplets onto fresh foliar material as ants incorporate it into the fungus garden. Although the enzymes concentrated by L. gongylophorus within gongylidia are thought to be critical to the initial degradation of plant biomass, only a few enzymes present in these hyphal swellings have been identified. Here we use proteomic methods to identify proteins present in the gongylidia of three Atta cephalotes colonies. Our results demonstrate that a diverse but consistent set of enzymes is present in gongylidia, including numerous plant biomass-degrading enzymes likely involved in the degradation of polysaccharides, plant toxins, and proteins. Overall, gongylidia contained over three quarters of all biomass-degrading enzymes identified in the L. gongylophorus genome, demonstrating that the majority of the enzymes produced by this fungus for biomass breakdown are ingested by the ants. We also identify a set of 40 of these enzymes enriched in gongylidia compared to whole fungus garden samples, suggesting that certain enzymes may be particularly important in the initial degradation of foliar material. Our work sheds light on the complex interplay between leaf-cutter ants and their fungal symbiont that allows for the host insects to occupy an herbivorous niche by indirectly deriving energy from plant biomass.

  20. Enrichment and broad representation of plant biomass-degrading enzymes in the specialized hyphal swellings of Leucoagaricus gongylophorus, the fungal symbiont of leaf-cutter ants

    DOE PAGES

    Aylward, Frank O.; Khadempour, Lily; Tremmel, Daniel M.; McDonald, Bradon R.; Nicora, Carrie D.; Wu, Si; Moore, Ronald J.; Orton, Daniel J.; Monroe, Matthew E.; Piehowski, Paul D.; et al

    2015-08-28

    Leaf-cutter ants are prolific and conspicuous constituents of Neotropical ecosystems that derive energy from specialized fungus gardens they cultivate using prodigious amounts of foliar biomass. The basidiomycetous cultivar of the ants, Leucoagaricus gongylophorus, produces specialized hyphal swellings called gongylidia that serve as the primary food source of ant colonies. Gongylidia also contain plant biomass-degrading enzymes that become concentrated in ant digestive tracts and are deposited within fecal droplets onto fresh foliar material as ants incorporate it into the fungus garden. Although the enzymes concentrated by L. gongylophorus within gongylidia are thought to be critical to the initial degradation of plantmore » biomass, only a few enzymes present in these hyphal swellings have been identified. Here we use proteomic methods to identify proteins present in the gongylidia of three Atta cephalotes colonies. Our results demonstrate that a diverse but consistent set of enzymes is present in gongylidia, including numerous plant biomass-degrading enzymes likely involved in the degradation of polysaccharides, plant toxins, and proteins. Overall, gongylidia contained over three quarters of all biomass-degrading enzymes identified in the L. gongylophorus genome, demonstrating that the majority of the enzymes produced by this fungus for biomass breakdown are ingested by the ants. We also identify a set of 40 of these enzymes enriched in gongylidia compared to whole fungus garden samples, suggesting that certain enzymes may be particularly important in the initial degradation of foliar material. Our work sheds light on the complex interplay between leaf-cutter ants and their fungal symbiont that allows for the host insects to occupy an herbivorous niche by indirectly deriving energy from plant biomass.« less

  1. Enrichment and Broad Representation of Plant Biomass-Degrading Enzymes in the Specialized Hyphal Swellings of Leucoagaricus gongylophorus, the Fungal Symbiont of Leaf-Cutter Ants

    PubMed Central

    Aylward, Frank O.; Khadempour, Lily; Tremmel, Daniel M.; McDonald, Bradon R.; Nicora, Carrie D.; Wu, Si; Moore, Ronald J.; Orton, Daniel J.; Monroe, Matthew E.; Piehowski, Paul D.; Purvine, Samuel O.; Smith, Richard D.; Lipton, Mary S.; Burnum-Johnson, Kristin E.; Currie, Cameron R.

    2015-01-01

    Leaf-cutter ants are prolific and conspicuous constituents of Neotropical ecosystems that derive energy from specialized fungus gardens they cultivate using prodigious amounts of foliar biomass. The basidiomycetous cultivar of the ants, Leucoagaricus gongylophorus, produces specialized hyphal swellings called gongylidia that serve as the primary food source of ant colonies. Gongylidia also contain plant biomass-degrading enzymes that become concentrated in ant digestive tracts and are deposited within fecal droplets onto fresh foliar material as ants incorporate it into the fungus garden. Although the enzymes concentrated by L. gongylophorus within gongylidia are thought to be critical to the initial degradation of plant biomass, only a few enzymes present in these hyphal swellings have been identified. Here we use proteomic methods to identify proteins present in the gongylidia of three Atta cephalotes colonies. Our results demonstrate that a diverse but consistent set of enzymes is present in gongylidia, including numerous plant biomass-degrading enzymes likely involved in the degradation of polysaccharides, plant toxins, and proteins. Overall, gongylidia contained over three quarters of all biomass-degrading enzymes identified in the L. gongylophorus genome, demonstrating that the majority of the enzymes produced by this fungus for biomass breakdown are ingested by the ants. We also identify a set of 40 of these enzymes enriched in gongylidia compared to whole fungus garden samples, suggesting that certain enzymes may be particularly important in the initial degradation of foliar material. Our work sheds light on the complex interplay between leaf-cutter ants and their fungal symbiont that allows for the host insects to occupy an herbivorous niche by indirectly deriving energy from plant biomass. PMID:26317212

  2. Aerial view of the Kennedy Space Center Visitor Center

    NASA Technical Reports Server (NTRS)

    1998-01-01

    The Kennedy Space Center Visitor Complex, shown in this aerial view looking east, sprawls across 70 acres on Florida's Space Coast. It is located off State Road 405, NASA Parkway, six miles inside the Space Center entrance. SR 405 can be seen at the top left of the photo. In the foreground is the display of rockets that have played a significant role in the growth of the space program. Just above that, left to right, can be seen the Theater Complex, Space Flight Exhibit Building and Spaceport Central. Other buildings clustered at the center are the Cafeteria, Souvenir Sales Building, and Ticket Pavilion. To the left of the Theater Complex are the Astronaut Memorial, the Post Show Dome, and the Shuttle/Gantry mockup. Not seen in the photo is the Center for Space Education.

  3. Aerial view of the Kennedy Space Center Visitor Center

    NASA Technical Reports Server (NTRS)

    1998-01-01

    The Kennedy Space Center Visitor Complex, shown in this aerial view looking south, sprawls across 70 acres on Florida's Space Coast. It is located off State Road 405, NASA Parkway, six miles inside the Space Center entrance. SR 405 can be seen at the bottom of the photo. Just above the roadway, from left, can be seen the Shuttle/Gantry mockup, the Post Show Dome, the Astronaut Memorial, and to the far right, the Center for Space Education. Behind the Memorial are a cluster of buildings that include the Theater Complex, Cafeteria, Space Flight Exhibit Building, Souvenir Sales Building, Spaceport Central, and Ticket Pavilion. At the upper right of the site is a display of rockets that have played a significant role in the growth of the space program. Parking lots span the width of the complex on the south side.

  4. Aerial view of the Kennedy Space Center Visitor Center

    NASA Technical Reports Server (NTRS)

    1998-01-01

    The Kennedy Space Center Visitor Center, shown in this aerial view looking northwest, sprawls across 70 acres on Florida's Space Coast and is located off State Road 405, NASA Parkway, six miles inside the Space Center entrance. SR 405 can be seen at the top of the photo (left to right). Just below the roadway, from left, can be seen the Center for Space Education, the Theater Complex, Astronaut Memorial, the Post Show Dome, and Shuttle/Gantry mockup. In front of the theater complex are a cluster of buildings that include the Cafeteria, Space Flight Exhibit Building, Souvenir Sales Building, Spaceport Central, and Ticket Pavilion. At the left of the complex are various rockets that have played a significant role in the growth of the space program. Beyond the roadway can be seen the Banana River.

  5. Aerial view of the Kennedy Space Center Visitor Center

    NASA Technical Reports Server (NTRS)

    1998-01-01

    The Kennedy Space Center Visitor Center, shown in this aerial view looking south, sprawls across 70 acres on Florida's Space Coast , and is located off State Road 405, NASA Parkway, six miles inside the Space Center entrance. SR 405 can be seen at the bottom of the photo. Just above the roadway, from left can be seen the Shuttle/Gantry mockup; the Post Show Dome; the Astronaut Memorial; and to the far right, the Center for Space Education. Behind the Memorial are a cluster of buildings that include the Theater Complex, Cafeteria, Space Flight Exhibit Building, Souvenir Sales Building, Spaceport Central, and Ticket Pavilion. At the upper right are various rockets that have played a significant role in the growth of the space program.

  6. Morphological Characterization and Quantification of the Mycelial Growth of the Brown-Rot Fungus Postia placenta for Modeling Purposes.

    PubMed

    Du, Huan; Lv, Pin; Ayouz, Mehdi; Besserer, Arnaud; Perré, Patrick

    2016-01-01

    Continuous observation was performed using confocal laser scanning microscopy to visualize the three-dimensional microscopic growth of the brown-rot fungus, Postia placenta, for seventeen days. The morphological characterization of Postia placenta was quantitatively determined, including the tip extension rate, branch angle and branching length, (hyphal length between two adjacent branch sites). A voxel method has been developed to measure the growth of the biomass. Additionally, the tip extension rate distribution, the branch angle distribution and the branching length distribution, which quantified the hyphal growth characteristics, were evaluated. Statistical analysis revealed that the extension rate of tips was randomly distributed in space. The branch angle distribution did not change with the development of the colony, however, the branching length distribution did vary with the development of the colony. The experimental data will be incorporated into a lattice-based model simulating the growth of Postia placenta. PMID:27602575

  7. Morphological Characterization and Quantification of the Mycelial Growth of the Brown-Rot Fungus Postia placenta for Modeling Purposes

    PubMed Central

    Lv, Pin; Ayouz, Mehdi; Besserer, Arnaud; Perré, Patrick

    2016-01-01

    Continuous observation was performed using confocal laser scanning microscopy to visualize the three-dimensional microscopic growth of the brown-rot fungus, Postia placenta, for seventeen days. The morphological characterization of Postia placenta was quantitatively determined, including the tip extension rate, branch angle and branching length, (hyphal length between two adjacent branch sites). A voxel method has been developed to measure the growth of the biomass. Additionally, the tip extension rate distribution, the branch angle distribution and the branching length distribution, which quantified the hyphal growth characteristics, were evaluated. Statistical analysis revealed that the extension rate of tips was randomly distributed in space. The branch angle distribution did not change with the development of the colony, however, the branching length distribution did vary with the development of the colony. The experimental data will be incorporated into a lattice-based model simulating the growth of Postia placenta. PMID:27602575

  8. The remote sensing of aquatic macrophytes Part 1: Color-infrared aerial photography as a tool for identification and mapping of littoral vegetation. Part 2: Aerial photography as a quantitative tool for the investigation of aquatic ecosystems. [Lake Wingra, Wisconsin

    NASA Technical Reports Server (NTRS)

    Gustafson, T. D.; Adams, M. S.

    1973-01-01

    Research was initiated to use aerial photography as an investigative tool in studies that are part of an intensive aquatic ecosystem research effort at Lake Wingra, Madison, Wisconsin. It is anticipated that photographic techniques would supply information about the growth and distribution of littoral macrophytes with efficiency and accuracy greater than conventional methods.

  9. Differential roles of pyruvate decarboxylase in aerial and embedded mycelia of the ascomycete Gibberella zeae.

    PubMed

    Son, Hokyoung; Min, Kyunghun; Lee, Jungkwan; Choi, Gyung Ja; Kim, Jin-Cheol; Lee, Yin-Won

    2012-04-01

    The pyruvate-acetaldehyde-acetate (PAA) pathway has diverse roles in eukaryotes. Our previous study on acetyl-coenzyme A synthetase 1 (ACS1) in Gibberella zeae suggested that the PAA pathway is important for lipid production, which is required for perithecia maturation. In this study, we deleted all three pyruvate decarboxylase (PDC) genes, which encode enzymes that function upstream of ACS1 in the PAA pathway. Results suggest PDC1 is required for lipid accumulation in the aerial mycelia, and deletion of PDC1 resulted in highly wettable mycelia. However, the total amount of lipids in the PDC1 deletion mutants was similar to that of the wild-type strain, likely due to compensatory lipid production processes in the embedded mycelia. PDC1 was expressed both in the aerial and embedded mycelia, whereas ACS1 was observed only in the aerial mycelia in a PDC1-dependent manner. PDC1 is also involved in vegetative growth of embedded mycelia in G. zeae, possibly through initiating the ethanol fermentation pathway. Thus, PDC1 may function as a key metabolic enzyme crucial for lipid production in the aerial mycelia, but play a different role in the embedded mycelia, where it might be involved in energy generation by ethanol fermentation.

  10. Controller Design of Quadrotor Aerial Robot

    NASA Astrophysics Data System (ADS)

    Yali, Yu; SunFeng; Yuanxi, Wang

    This paper deduced the nonlinear dynamic model of a quadrotor aerial robot, which was a VTOL (vertical tale-off and landing) unmanned air vehicle. Since that is a complex model with the highly nonlinear multivariable strongly coupled and under-actuated property, the controller design of it was very difficult. Aimed at attaining the excellent controller, the whole system can be divided into three interconnected parts: attitude subsystem, vertical subsystem, position subsystem. Then nonlinear control strategy of them has been described, such as SDRE and Backstepping. The controller design was presented to stabilize the whole system. Through simulation result indicates, the various models have shown that the control law stabilize a quadrotor aerial robot with good tracking performance and robotness of the system.

  11. Aerial color infrared photography applications to citriculture

    NASA Technical Reports Server (NTRS)

    Blazquez, C. H.; Horn, F. W., Jr.

    1980-01-01

    Results of a one-year experimental study on the use of aerial color infrared photography in citrus grove management are presented. It is found that the spring season, when trees are in flush (have young leaves), is the best season to photograph visible differences between healthy and diseased trees. It is also shown that the best photography can be obtained with a 12-in. focal length lens. The photographic scale that allowed good photo interpretation with simple inexpensive equipment was 1 in. = 330 ft. The use of a window-overlay transparency method allowed rapid photo interpretation and data recording in computer-compatible forms. Aerial color infrared photography carried out during the spring season revealed a more accurate status of tree condition than visual inspection.

  12. Ultralight photovoltaic modules for unmanned aerial vehicles

    SciTech Connect

    Nowlan, M.J.; Maglitta, J.C.; Darkazalli, G.; Lamp, T.

    1997-12-31

    New lightweight photovoltaic modules are being developed for powering high altitude unmanned aerial vehicles (UAVs). Modified low-cost terrestrial solar cell and module technologies are being applied to minimize vehicle cost. New processes were developed for assembling thin solar cells, encapsulant films, and cover films. An innovative by-pass diode mounting approach that uses a solar cell as a heat spreader was devised and tested. Materials and processes will be evaluated through accelerated environmental testing.

  13. Toxicological effects of aerial application of monocrotophos.

    PubMed

    Rao, R R; Quadros, F; Mazmudar, R M; Marathe, M R; Gangoli, S D

    1980-01-01

    Aerial application of the insecticide Nuvacron 40% (monocrotophos) had no significant effect on the cholinesterase level of plasma and erythrocytes of cattle, chicken, buffaloes, and human volunteers exposed to the spray. Contamination of canal water with the pesticide was completely eliminated within 24 hr, whereas that in the soil was reduced by 80% in 72 hr. The degradation of insecticide residue in grass was about 90% in seven days and in cotton leaves about 85% for the same period.

  14. Comparative Analysis of the Tour Jete and Aerial with Detailed Analysis of Aerial Takeoff Mechanics

    NASA Astrophysics Data System (ADS)

    Pierson, Mimi; Coplin, Kim

    2006-10-01

    Whether internally as muscle tension or from external sources, forces are necessary for all motion. This research focused on athletic rotations where conditions of flight are established during takeoff. By studying reaction forces that produce torques, moments of inertia, and linear and angular differences between distinct rotations around different principle axes of the body (tour jete in ballet - longitudinal axis; aerial in gymnastics - anteroposterior axis), and by looking at the values of angular momentum in the specific mechanics of aerial takeoff, we can gain insight into possible causes of injury, flaws in technique and limitations of athletes. Results showed significant differences in the horizontal and vertical components of takeoff between the tour jete and the aerial, and a realization that torque was produced in different biomechanical planes. Both rotations showed braking forces before takeoff to counteract forward momentum and increase vertical lift, but the angle of applied force varied, and the horizontal components of velocity and force and vertical velocity as well as moment of inertia throughout flight were consistently greater for the aerial. Breakdown of aerial takeoff highlighted the relative importance of the takeoff phases, showing that completion depends fundamentally upon the rotation of the rear foot and torso twisting during takeoff rather than the last foot in contact with the ground.

  15. Inertial instrument system for aerial surveying

    USGS Publications Warehouse

    Brown, R.H.; Chapman, W.H.; Hanna, W.F.; Mongan, C.E.; Hursh, J.W.

    1985-01-01

    An inertial guidance system for aerial surveying has been developed under contract to the U.S. Geological Survey. This prototype system, known as the aerial profiling of terrain (APT) system, is designed to determine continuously the positions of points along an aircraft flight path, or the underlying terrain profile, to an accuracy of + or - 0.5 ft (15 cm) vertically and + or - 2 ft (61 cm) horizontally. The system 's objective thus is to accomplish, from a fixed-wing aircraft, what would traditionally be accomplished from ground-based topographic surveys combined with aerial photography and photogrammetry. The two-part strategy for measuring the terrain profile entails: (1) use of an inertial navigator for continuous determination of the three-coordinate position of the aircraft, and (2) use of an eye-safe pulsed laser profiler for continuous measurement of the vertical distance from aircraft to land surface, so that the desired terrain profile can then be directly computed. The APT system, installed in a DeHavilland Twin Otter aircraft, is typically flown at a speed of 115 mph (105 knots) at an altitude of 2,000 ft (610 m) above the terrain. Performance-evaluation flights have shown that the vertical and horizontal accuracy specifications are met. (USGS)

  16. Mask degradation monitoring with aerial mask inspector

    NASA Astrophysics Data System (ADS)

    Tseng, Wen-Jui; Fu, Yung-Ying; Lu, Shih-Ping; Jiang, Ming-Sian; Lin, Jeffrey; Wu, Clare; Lifschitz, Sivan; Tam, Aviram

    2013-06-01

    As design rule continues to shrink, microlithography is becoming more challenging and the photomasks need to comply with high scanner laser energy, low CDU, and ever more aggressive RETs. This give rise to numerous challenges in the semiconductor wafer fabrication plants. Some of these challenges being contamination (mainly haze and particles), mask pattern degradation (MoSi oxidation, chrome migration, etc.) and pellicle degradation. Fabs are constantly working to establish an efficient methodology to manage these challenges mainly using mask inspection, wafer inspection, SEM review and CD SEMs. Aerial technology offers a unique opportunity to address the above mask related challenges using one tool. The Applied Materials Aera3TM system has the inherent ability to inspect for defects (haze, particles, etc.), and track mask degradation (e.g. CDU). This paper focuses on haze monitoring, which is still a significant challenge in semiconductor manufacturing, and mask degradation effects that are starting to emerge as the next challenge for high volume semiconductor manufacturers. The paper describes Aerial inspector (Aera3) early haze methodology and mask degradation tracking related to high volume manufacturing. These will be demonstrated on memory products. At the end of the paper we take a brief look on subsequent work currently conducted on the more general issue of photo mask degradation monitoring by means of an Aerial inspector.

  17. Localization of aerial broadband noise by pinnipeds

    NASA Astrophysics Data System (ADS)

    Holt, Marla M.; Schusterman, Ronald J.; Southall, Brandon L.; Kastak, David

    2004-05-01

    Although many pinnipeds (seals, sea lions, and walruses) emit broadband calls on land as part of their communication system, few studies have addressed these animals' ability to localize aerial broadband sounds. In this study, the aerial sound localization acuities of a female northern elephant seal (Mirounga angustirostris), a male harbor seal (Phoca vitulina), and a female California sea lion (Zalophus californianus) were measured in the horizontal plane. The stimulus was broadband white noise that was band pass filtered between 1.2 and 15 kHz. Testing was conducted in a hemi-anechoic chamber using a left/right forced choice procedure to measure the minimum audible angle (MAA) for each subject. MAAs were defined as half the angular separation of two sound sources bisected by a subject's midline that corresponded to 75% correct discrimination. MAAs were 4.7°, 3.6°, and 4.2° for the northern elephant seal, harbor seal, and California sea lion, respectively. These results demonstrate that individuals of these pinniped species have sound localization abilities comparable to the domestic cat and rhesus macaque. The acuity differences between our subjects were small and not predicted by head size. These results likely reflect the relatively acute general abilities of pinnipeds to localize aerial broadband signals.

  18. Remotely deployable aerial inspection using tactile sensors

    SciTech Connect

    MacLeod, C. N.; Cao, J.; Pierce, S. G.; Dobie, G.; Summan, R.; Sullivan, J. C.; Pipe, A. G.

    2014-02-18

    For structural monitoring applications, the use of remotely deployable Non-Destructive Evaluation (NDE) inspection platforms offer many advantages, including improved accessibility, greater safety and reduced cost, when compared to traditional manual inspection techniques. The use of such platforms, previously reported by researchers at the University Strathclyde facilitates the potential for rapid scanning of large areas and volumes in hazardous locations. A common problem for both manual and remote deployment approaches lies in the intrinsic stand-off and surface coupling issues of typical NDE probes. The associated complications of these requirements are obviously significantly exacerbated when considering aerial based remote inspection and deployment, resulting in simple visual techniques being the preferred sensor payload. Researchers at Bristol Robotics Laboratory have developed biomimetic tactile sensors modelled on the facial whiskers (vibrissae) of animals such as rats and mice, with the latest sensors actively sweeping their tips across the surface in a back and forth motion. The current work reports on the design and performance of an aerial inspection platform and the suitability of tactile whisking sensors to aerial based surface monitoring applications.

  19. Effects of Clitoria ternatea leaf extract on growth and morphogenesis of Aspergillus niger.

    PubMed

    Kamilla, L; Mansor, S M; Ramanathan, S; Sasidharan, S

    2009-08-01

    Clitoria ternatea is known for its antimicrobial activity but the antifungal effects of leaf extract on growth and morphogenesis of Aspergillus niger have not been observed. The extract showed a favorable antifungal activity against A. niger with a minimum inhibition concentration 0.8 mg/mL and minimum fungicidal concentration 1.6 mg/mL, respectively. The leaf extract exhibited considerable antifungal activity against filamentous fungi in a dose-dependent manner with 0.4 mg/mL IC50 value on hyphal growth of A. niger. The main changes observed under scanning electron microscopy after C. ternatea extract treatment were loss of cytoplasm in fungal hyphae and the hyphal wall and its diameter became markedly thinner, distorted, and resulted in cell wall disruption. In addition, conidiophore alterations were also observed when A. niger was treated with C. ternatea leaf extract. PMID:19575837

  20. Unmanned aerial optical systems for spatial monitoring of Antarctic mosses

    NASA Astrophysics Data System (ADS)

    Lucieer, Arko; Turner, Darren; Veness, Tony; Malenovsky, Zbynek; Harwin, Stephen; Wallace, Luke; Kelcey, Josh; Robinson, Sharon

    2013-04-01

    The Antarctic continent has experienced major changes in temperature, wind speed and stratospheric ozone levels during the last 50 years. In a manner similar to tree rings, old growth shoots of Antarctic mosses, the only plants on the continent, also preserve a climate record of their surrounding environment. This makes them an ideal bio-indicator of the Antarctic climate change. Spatially extensive ground sampling of mosses is laborious and time limited due to the short Antarctic growing season. Obviously, there is a need for an efficient method to monitor spatially climate change induced stress of the Antarctic moss flora. Cloudy weather and high spatial fragmentation of the moss turfs makes satellite imagery unsuitable for this task. Unmanned aerial systems (UAS), flying at low altitudes and collecting image data even under a full overcast, can, however, overcome the insufficiency of satellite remote sensing. We, therefore, developed scientific UAS, consisting of a remote-controlled micro-copter carrying on-board different remote sensing optical sensors, tailored to perform fast and cost-effective mapping of Antarctic flora at ultra-high spatial resolution (1-10 cm depending on flight altitude). A single lens reflex (SLR) camera carried by UAS acquires multi-view aerial photography, which processed by the Structure from Motion computer vision algorithm provides an accurate three-dimensional digital surface model (DSM) at ultra-high spatial resolution. DSM is the key input parameter for modelling a local seasonal snowmelt run-off, which provides mosses with the vital water supply. A lightweight multispectral camera on-board of UVS is collecting images of six selected spectral wavebands with the full-width-half-maximum (FWHM) of 10 nm. The spectral bands can be used to compute various vegetation optical indices, e.g. Difference Vegetation Index (NDVI) or Photochemical Reflectance Index (PRI), assessing the actual physiological state of polar vegetation. Recently

  1. Biomechanical responses of aquatic plants to aerial conditions

    PubMed Central

    Hamann, Elena; Puijalon, Sara

    2013-01-01

    Background and Aims Wetlands are impacted by changes in hydrological regimes that can lead to periods of low water levels. During these periods, aquatic plants experience a drastic change in the mechanical conditions that they encounter, from low gravitational and tensile hydrodynamic forces when exposed to flow under aquatic conditions, to high gravitational and bending forces under terrestrial conditions. The objective of this study was to test the capacity of aquatic plants to produce self-supporting growth forms when growing under aerial conditions by assessing their resistance to terrestrial mechanical conditions and the associated morpho-anatomical changes. Methods Plastic responses to aerial conditions were assessed by sampling Berula erecta, Hippuris vulgaris, Juncus articulatus, Lythrum salicaria, Mentha aquatica, Myosotis scorpioides, Nuphar lutea and Sparganium emersum under submerged and emergent conditions. The cross-sectional area and dry matter content (DMC) were measured in the plant organs that bear the mechanical forces, and their biomechanical properties in tension and bending were assessed. Key Results All of the species except for two had significantly higher stiffness in bending and thus an increased resistance to terrestrial mechanical conditions when growing under emergent conditions. This response was determined either by an increased allocation to strengthening tissues and thus a higher DMC, or by an increased cross-sectional area. These morpho-anatomical changes also resulted in increased strength and stiffness in tension. Conclusions The capacity of the studied species to colonize this fluctuating environment can be accounted for by a high degree of phenotypic plasticity in response to emersion. Further investigation is however needed to disentangle the finer mechanisms behind these responses (e.g. allometric relations, tissue make-up), their costs and adaptive value. PMID:24187030

  2. Identification of some diterpenoids and hydroxy fatty acids from carrot root cell walls that stimulate the presymbiotic hyphal growth of AM fungi

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Arbuscular mycorrhizal (AM) fungi infect about 80% of all land plants (Smith and Read 1997). They are soil borne and establish a mutually beneficial symbiotic association with a host root after colonization. The fungal hyphae from a colonized root can more thoroughly explore the soil than the root...

  3. Arctic Oil Spill Mapping and Response Using Unmanned Aerial Systems

    NASA Astrophysics Data System (ADS)

    Cunningham, K. W.

    2011-12-01

    The University of Alaska Fairbanks works extensively with unmanned aerial systems and various sensor payloads used in mapping. Recent projects with Royal Dutch Shell and British Petroleum have demonstrated that unmanned aerial systems, including fixed and rotary winged platforms, can provide quick response to oil spill mapping in a variety of flight conditions, including those not well suited for manned aerial systems. We describe this collaborative research between the University and oil companies exploring and developing oil resources in Alaska and the Arctic.

  4. AERIAL VIEW FACING NORTH. DOWNSTREAM VIEW OF FABRIC BUILDING, STRUCTURAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AERIAL VIEW FACING NORTH. DOWNSTREAM VIEW OF FABRIC BUILDING, STRUCTURAL WAREHOUSE, RAIL MILL, & OPEN HEARTH COMPLEX. - Pittsburgh Steel Company, Monessen Works, Donner Avenue, Monessen, Westmoreland County, PA

  5. 21. AERIAL VIEW LOOKING EAST TOWARDS LINCOLN MEMORIAL AND WASHINGTON ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. AERIAL VIEW LOOKING EAST TOWARDS LINCOLN MEMORIAL AND WASHINGTON MONUMENT - Arlington Memorial Bridge, Spanning Potomac River between Lincoln Memorial & Arlington National Cemetery, Washington, District of Columbia, DC

  6. 20. AERIAL VIEW LOOKING NORTH FROM ARLINGTON TOWARDS LINCOLN MEMORIAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. AERIAL VIEW LOOKING NORTH FROM ARLINGTON TOWARDS LINCOLN MEMORIAL - Arlington Memorial Bridge, Spanning Potomac River between Lincoln Memorial & Arlington National Cemetery, Washington, District of Columbia, DC

  7. Discovering discriminative graphlets for aerial image categories recognition.

    PubMed

    Zhang, Luming; Han, Yahong; Yang, Yi; Song, Mingli; Yan, Shuicheng; Tian, Qi

    2013-12-01

    Recognizing aerial image categories is useful for scene annotation and surveillance. Local features have been demonstrated to be robust to image transformations, including occlusions and clutters. However, the geometric property of an aerial image (i.e., the topology and relative displacement of local features), which is key to discriminating aerial image categories, cannot be effectively represented by state-of-the-art generic visual descriptors. To solve this problem, we propose a recognition model that mines graphlets from aerial images, where graphlets are small connected subgraphs reflecting both the geometric property and color/texture distribution of an aerial image. More specifically, each aerial image is decomposed into a set of basic components (e.g., road and playground) and a region adjacency graph (RAG) is accordingly constructed to model their spatial interactions. Aerial image categories recognition can subsequently be casted as RAG-to-RAG matching. Based on graph theory, RAG-to-RAG matching is conducted by comparing all their respective graphlets. Because the number of graphlets is huge, we derive a manifold embedding algorithm to measure different-sized graphlets, after which we select graphlets that have highly discriminative and low redundancy topologies. Through quantizing the selected graphlets from each aerial image into a feature vector, we use support vector machine to discriminate aerial image categories. Experimental results indicate that our method outperforms several state-of-the-art object/scene recognition models, and the visualized graphlets indicate that the discriminative patterns are discovered by our proposed approach. PMID:23955764

  8. 1. Aerial view, looking northeast up Newark Bay, showing entire ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Aerial view, looking northeast up Newark Bay, showing entire island Charles Wisniewski, photographer, January 1985 - Shooters Island, Ships Graveyard, Newark Bay, Staten Island (subdivision), Richmond County, NY

  9. Aerial and soil seed banks enable populations of an annual species to cope with an unpredictable dune ecosystem

    PubMed Central

    Gao, Ruiru; Yang, Xuejun; Yang, Fan; Wei, Lingling; Huang, Zhenying; Walck, Jeffrey L.

    2014-01-01

    Background and Aims Simultaneous formation of aerial and soil seed banks by a species provides a mechanism for population maintenance in unpredictable environments. Eolian activity greatly affects growth and regeneration of plants in a sand dune system, but we know little about the difference in the contributions of these two seed banks to population dynamics in sand dunes. Methods Seed release, germination, seedling emergence and survival of a desert annual, Agriophyllum squarrosum (Chenopodiaceae), inhabiting the Ordos Sandland in China, were determined in order to explore the different functions of the aerial and soil seed banks. Key Results The size of the aerial seed bank was higher than that of the soil seed bank throughout the growing season. Seed release was positively related to wind velocity. Compared with the soil seed bank, seed germination from the aerial seed bank was lower at low temperature (5/15 °C night/day) but higher in the light. Seedling emergence from the soil seed bank was earlier than that from the aerial seed bank. Early-emerged (15 April–15 May) seedlings died due to frost, but seedlings that emerged during the following months survived to reproduce successfully. Conclusions The timing of seed release and different germination behaviour resulted in a temporal heterogeneity of seedling emergence and establishment between the two seed banks. The study suggests that a bet-hedging strategy for the two seed banks enables A. squarrosum populations to cope successfully with the unpredictable desert environment. PMID:24918206

  10. D Surface Generation from Aerial Thermal Imagery

    NASA Astrophysics Data System (ADS)

    Khodaei, B.; Samadzadegan, F.; Dadras Javan, F.; Hasani, H.

    2015-12-01

    Aerial thermal imagery has been recently applied to quantitative analysis of several scenes. For the mapping purpose based on aerial thermal imagery, high accuracy photogrammetric process is necessary. However, due to low geometric resolution and low contrast of thermal imaging sensors, there are some challenges in precise 3D measurement of objects. In this paper the potential of thermal video in 3D surface generation is evaluated. In the pre-processing step, thermal camera is geometrically calibrated using a calibration grid based on emissivity differences between the background and the targets. Then, Digital Surface Model (DSM) generation from thermal video imagery is performed in four steps. Initially, frames are extracted from video, then tie points are generated by Scale-Invariant Feature Transform (SIFT) algorithm. Bundle adjustment is then applied and the camera position and orientation parameters are determined. Finally, multi-resolution dense image matching algorithm is used to create 3D point cloud of the scene. Potential of the proposed method is evaluated based on thermal imaging cover an industrial area. The thermal camera has 640×480 Uncooled Focal Plane Array (UFPA) sensor, equipped with a 25 mm lens which mounted in the Unmanned Aerial Vehicle (UAV). The obtained results show the comparable accuracy of 3D model generated based on thermal images with respect to DSM generated from visible images, however thermal based DSM is somehow smoother with lower level of texture. Comparing the generated DSM with the 9 measured GCPs in the area shows the Root Mean Square Error (RMSE) value is smaller than 5 decimetres in both X and Y directions and 1.6 meters for the Z direction.

  11. BOREAS Level-0 C-130 Aerial Photography

    NASA Technical Reports Server (NTRS)

    Newcomer, Jeffrey A.; Dominguez, Roseanne; Hall, Forrest G. (Editor)

    2000-01-01

    For BOReal Ecosystem-Atmosphere Study (BOREAS), C-130 and other aerial photography was collected to provide finely detailed and spatially extensive documentation of the condition of the primary study sites. The NASA C-130 Earth Resources aircraft can accommodate two mapping cameras during flight, each of which can be fitted with 6- or 12-inch focal-length lenses and black-and-white, natural-color, or color-IR film, depending upon requirements. Both cameras were often in operation simultaneously, although sometimes only the lower resolution camera was deployed. When both cameras were in operation, the higher resolution camera was often used in a more limited fashion. The acquired photography covers the period of April to September 1994. The aerial photography was delivered as rolls of large format (9 x 9 inch) color transparency prints, with imagery from multiple missions (hundreds of prints) often contained within a single roll. A total of 1533 frames were collected from the C-130 platform for BOREAS in 1994. Note that the level-0 C-130 transparencies are not contained on the BOREAS CD-ROM set. An inventory file is supplied on the CD-ROM to inform users of all the data that were collected. Some photographic prints were made from the transparencies. In addition, BORIS staff digitized a subset of the tranparencies and stored the images in JPEG format. The CD-ROM set contains a small subset of the collected aerial photography that were the digitally scanned and stored as JPEG files for most tower and auxiliary sites in the NSA and SSA. See Section 15 for information about how to acquire additional imagery.

  12. Photogrammetric mapping using unmanned aerial vehicle

    NASA Astrophysics Data System (ADS)

    Graça, N.; Mitishita, E.; Gonçalves, J.

    2014-11-01

    Nowadays Unmanned Aerial Vehicle (UAV) technology has attracted attention for aerial photogrammetric mapping. The low cost and the feasibility to automatic flight along commanded waypoints can be considered as the main advantages of this technology in photogrammetric applications. Using GNSS/INS technologies the images are taken at the planned position of the exposure station and the exterior orientation parameters (position Xo, Yo, Zo and attitude ω, φ, χ) of images can be direct determined. However, common UAVs (off-the-shelf) do not replace the traditional aircraft platform. Overall, the main shortcomings are related to: difficulties to obtain the authorization to perform the flight in urban and rural areas, platform stability, safety flight, stability of the image block configuration, high number of the images and inaccuracies of the direct determination of the exterior orientation parameters of the images. In this paper are shown the obtained results from the project photogrammetric mapping using aerial images from the SIMEPAR UAV system. The PIPER J3 UAV Hydro aircraft was used. It has a micro pilot MP2128g. The system is fully integrated with 3-axis gyros/accelerometers, GPS, pressure altimeter, pressure airspeed sensors. A Sony Cyber-shot DSC-W300 was calibrated and used to get the image block. The flight height was close to 400 m, resulting GSD near to 0.10 m. The state of the art of the used technology, methodologies and the obtained results are shown and discussed. Finally advantages/shortcomings found in the study and main conclusions are presented

  13. An aerial 3D printing test mission

    NASA Astrophysics Data System (ADS)

    Hirsch, Michael; McGuire, Thomas; Parsons, Michael; Leake, Skye; Straub, Jeremy

    2016-05-01

    This paper provides an overview of an aerial 3D printing technology, its development and its testing. This technology is potentially useful in its own right. In addition, this work advances the development of a related in-space 3D printing technology. A series of aerial 3D printing test missions, used to test the aerial printing technology, are discussed. Through completing these test missions, the design for an in-space 3D printer may be advanced. The current design for the in-space 3D printer involves focusing thermal energy to heat an extrusion head and allow for the extrusion of molten print material. Plastics can be used as well as composites including metal, allowing for the extrusion of conductive material. A variety of experiments will be used to test this initial 3D printer design. High altitude balloons will be used to test the effects of microgravity on 3D printing, as well as parabolic flight tests. Zero pressure balloons can be used to test the effect of long 3D printing missions subjected to low temperatures. Vacuum chambers will be used to test 3D printing in a vacuum environment. The results will be used to adapt a current prototype of an in-space 3D printer. Then, a small scale prototype can be sent into low-Earth orbit as a 3-U cube satellite. With the ability to 3D print in space demonstrated, future missions can launch production hardware through which the sustainability and durability of structures in space will be greatly improved.

  14. Aerial views of the San Andreas Fault

    USGS Publications Warehouse

    Moore, M.

    1988-01-01

    These aerial photographs of the San Andreas fault were taken in 1965 by Robert E. Wallace of the U.S Geological Survey. The pictures were taken with a Rolliflex camera on 20 format black and white flim; Wallace was aboard a light, fixed-wing aircraft, flying mostly at low altitudes. He photographed the fault from San Francisco near its north end where it enters by the Salton Sea. These images represent only a sampling of the more than 300 images prodcued during this project. All the photographs reside in the U.S Geological Survey Library in Menlo Park, California. 

  15. Aerial view of Runway 33 at SLF

    NASA Technical Reports Server (NTRS)

    2000-01-01

    This aerial view shows the approach on Runway 33 at the KSC Shuttle Landing Facility. The runway is 15,000 feet long, with 1,000-foot paved overruns at each end; 300 feet wide (about length of football field), with 50-foot asphalt shoulders each side; 16 inches thick in the center, and 15 inches thick on sides. It has a slope of 24 inches from the center line to the edge for drainage. The single landing strip is considered two runways, depending on approach -- Runway 15 from northwest, Runway 33 from southeast.

  16. Aerial view of the Press Site

    NASA Technical Reports Server (NTRS)

    1998-01-01

    In this aerial view, The News Center sits beyond a large parking lot, on a hill at the northeastern end of the Launch Complex 39 Area , next to the turn basin (at left). From left, the grandstand faces the launch pads several miles away on the Atlantic seashore; behind it, the television studio is the site of media conferences; next, the large white-roofed building is the hub of information and activity for press representatives. Lined up on the right of the Press Site are various buildings and trailers, home to major news networks. The parking lot can accommodate the hundreds of media personnel who attend Space Shuttle launches.

  17. Delivery of Unmanned Aerial Vehicle Data

    NASA Technical Reports Server (NTRS)

    Ivancic, William D.; Sullivan, Donald V.

    2011-01-01

    To support much of NASA's Upper Atmosphere Research Program science, NASA has acquired two Global Hawk Unmanned Aerial Vehicles (UAVs). Two major missions are currently planned using the Global Hawk: the Global Hawk Pacific (GloPac) and the Genesis and Rapid Intensification Processes (GRIP) missions. This paper briefly describes GloPac and GRIP, the concept of operations and the resulting requirements and communication architectures. Also discussed are requirements for future missions that may use satellite systems and networks owned and operated by third parties.

  18. Chemical structure of the cell-wall mannan of Candida albicans serotype A and its difference in yeast and hyphal forms

    PubMed Central

    Shibata, Nobuyuki; Suzuki, Akifumi; Kobayashi, Hidemitsu; Okawa, Yoshio

    2007-01-01

    The structure of the cell-wall mannan from the J-1012 (serotype A) strain of the polymorphic yeast Candida albicans was determined by acetolysis under mild conditions followed by HPLC and sequential NMR experiments. The serotype A mannan contained β-1,2-linked mannose residues attached to α-1,3-linked mannose residues and α-1,6-linked branching mannose residues. Using a β-1,2-mannosyltransferase, we synthesized a three-β-1,2-linkage-containing mannoheptaose and used it as a reference oligosaccharide for 1H-NMR assignment. On the basis of the results obtained, we derived an additivity rule for the 1H-NMR chemical shifts of the β-1,2-linked mannose residues. The morphological transformation of Candida cells from the yeast form to the hyphal form induced a significant decrease in the phosphodiesterified acid-labile β-1,2-linked manno-oligosaccharides, whereas the amount of acid-stable β-1,2 linkage-containing side chains did not change. These results suggest that the Candida mannan in candidiasis patients contains β-1,2-linked mannose residues and that they behave as a target of the immune system. PMID:17331070

  19. Isolation of Inositol Hexaphosphate (IHP)-Degrading Bacteria from Arbuscular Mycorrhizal Fungal Hyphal Compartments Using a Modified Baiting Method Involving Alginate Beads Containing IHP

    PubMed Central

    Hara, Shintaro; Saito, Masanori

    2016-01-01

    Phytate (inositol hexaphosphate; IHP)-degrading microbes have been suggested to contribute to arbuscular mycorrhizal fungi (AMF)-mediated P transfer from IHP to plants; however, no IHP degrader involved in AMF-mediated P transfer has been isolated to date. We herein report the isolation of IHP-degrading bacteria using a modified baiting method. We applied alginate beads as carriers of IHP powder, and used them as recoverable IHP in the AM fungal compartment of plant cultivation experiments. P transfer from IHP in alginate beads via AMF was confirmed, and extracted DNA from alginate beads was analyzed by denaturing gradient gel electrophoresis targeting the 16S rRNA gene and a clone library method for the beta-propeller phytase (BPP) gene. The diversities of the 16S rRNA and BPP genes of microbes growing on IHP beads were simple and those of Sphingomonas spp. and Caulobacter spp. dominated. A total of 187 IHP-utilizing bacteria were isolated and identified, and they were consistent with the results of DNA analysis. Furthermore, some isolated Sphingomonas spp. and Caulobacter sp. showed IHP-degrading activity. Therefore, we successfully isolated dominant IHP-degrading bacteria from IHP in an AMF hyphal compartment. These strains may contribute to P transfer from IHP via AMF. PMID:27383681

  20. Unmanned Aerial Vehicles unique cost estimating requirements

    NASA Astrophysics Data System (ADS)

    Malone, P.; Apgar, H.; Stukes, S.; Sterk, S.

    Unmanned Aerial Vehicles (UAVs), also referred to as drones, are aerial platforms that fly without a human pilot onboard. UAVs are controlled autonomously by a computer in the vehicle or under the remote control of a pilot stationed at a fixed ground location. There are a wide variety of drone shapes, sizes, configurations, complexities, and characteristics. Use of these devices by the Department of Defense (DoD), NASA, civil and commercial organizations continues to grow. UAVs are commonly used for intelligence, surveillance, reconnaissance (ISR). They are also use for combat operations, and civil applications, such as firefighting, non-military security work, surveillance of infrastructure (e.g. pipelines, power lines and country borders). UAVs are often preferred for missions that require sustained persistence (over 4 hours in duration), or are “ too dangerous, dull or dirty” for manned aircraft. Moreover, they can offer significant acquisition and operations cost savings over traditional manned aircraft. Because of these unique characteristics and missions, UAV estimates require some unique estimating methods. This paper describes a framework for estimating UAV systems total ownership cost including hardware components, software design, and operations. The challenge of collecting data, testing the sensitivities of cost drivers, and creating cost estimating relationships (CERs) for each key work breakdown structure (WBS) element is discussed. The autonomous operation of UAVs is especially challenging from a software perspective.

  1. Design of an integrated aerial image sensor

    NASA Astrophysics Data System (ADS)

    Xue, Jing; Spanos, Costas J.

    2005-05-01

    The subject of this paper is a novel integrated aerial image sensor (IAIS) system suitable for integration within the surface of an autonomous test wafer. The IAIS could be used as a lithography processing monitor, affording a "wafer's eye view" of the process, and therefore facilitating advanced process control and diagnostics without integrating (and dedicating) the sensor to the processing equipment. The IAIS is composed of an aperture mask and an array of photo-detectors. In order to retrieve nanometer scale resolution of the aerial image with a practical photo-detector pixel size, we propose a design of an aperture mask involving a series of spatial phase "moving" aperture groups. We demonstrate a design example aimed at the 65nm technology node through TEMPEST simulation. The optimized, key design parameters include an aperture width in the range of 30nm, aperture thickness in the range of 70nm, and offer a spatial resolution of about 5nm, all with comfortable fabrication tolerances. Our preliminary simulation work indicates the possibility of the IAIS being applied to the immersion lithography. A bench-top far-field experiment verifies that our approach of the spatial frequency down-shift through forming large Moire patterns is feasible.

  2. Community aerial mosquito control and naled exposure.

    PubMed

    Duprey, Zandra; Rivers, Samantha; Luber, George; Becker, Alan; Blackmore, Carina; Barr, Dana; Weerasekera, Gayanga; Kieszak, Stephanie; Flanders, W Dana; Rubin, Carol

    2008-03-01

    In October 2004, the Florida Department of Health (FLDOH) and the Centers for Disease Control and Prevention (CDC) assessed human exposure to ultra-low volume (ULV) aerial application of naled. Teams administered activity questionnaires regarding pesticide exposure and obtained baseline urine samples to quantify prespray naled metabolite levels. Following the spray event, participants were asked to collect postspray urine specimens within 12 h of the spray event and at 8-h intervals for up to 40 h. Upon completion, a postspray activity questionnaire was administered to study participants. Two hundred five (87%) participants completed the study. The urine analysis showed that although 67% of prespray urine samples had detectable levels of a naled metabolite, the majority of postspray samples were below the limit of detection (< LOD). Only at the "postspray 6" time period, which corresponds to a time greater than 5 half-lives (> 40 h) following exposure, the number of samples with detectable levels exceeded 50%. There was a significant decrease in naled metabolites from prespray to postspray (= .02), perhaps associated with a significant reduction (< or = 0.05) in some participants that may have resulted in pesticide exposure by means other than the mosquito control operations. These data suggest that aerial spraying of naled does not result in increased levels of naled in humans, provided the naled is used according to label instructions. PMID:18437813

  3. Moving Obstacle Avoidance for Unmanned Aerial Vehicles

    NASA Astrophysics Data System (ADS)

    Lin, Yucong

    There has been a vast increase in applications of Unmanned Aerial Vehicles (UAVs) in civilian domains. To operate in the civilian airspace, a UAV must be able to sense and avoid both static and moving obstacles for flight safety. While indoor and low-altitude environments are mainly occupied by static obstacles, risks in space of higher altitude primarily come from moving obstacles such as other aircraft or flying vehicles in the airspace. Therefore, the ability to avoid moving obstacles becomes a necessity for Unmanned Aerial Vehicles. Towards enabling a UAV to autonomously sense and avoid moving obstacles, this thesis makes the following contributions. Initially, an image-based reactive motion planner is developed for a quadrotor to avoid a fast approaching obstacle. Furthermore, A Dubin's curve based geometry method is developed as a global path planner for a fixed-wing UAV to avoid collisions with aircraft. The image-based method is unable to produce an optimal path and the geometry method uses a simplified UAV model. To compensate these two disadvantages, a series of algorithms built upon the Closed-Loop Rapid Exploratory Random Tree are developed as global path planners to generate collision avoidance paths in real time. The algorithms are validated in Software-In-the-Loop (SITL) and Hardware-In-the-Loop (HIL) simulations using a fixed-wing UAV model and in real flight experiments using quadrotors. It is observed that the algorithm enables a UAV to avoid moving obstacles approaching to it with different directions and speeds.

  4. Aerial survey estimates of fallow deer abundance

    USGS Publications Warehouse

    Gogan, Peter J.; Gates, Natalie B.; Lubow, Bruce C.; Pettit, Suzanne

    2012-01-01

    Reliable estimates of the distribution and abundance of an ungulate species is essential prior to establishing and implementing a management program. We used ground surveys to determine distribution and ground and aerial surveys and individually marked deer to estimate the abundance of fallow deer (Dama dama) in north-coastal California. Fallow deer had limited distribution and heterogeneous densities. Estimated post-rut densities across 4 annual surveys ranged from a low of 1.4 (SE=0.2) deer/km2 to a high of 3.3 (se=0.5) deer/km2 in a low density stratum and from 49.0 (SE=8.3) deer/km2 to 111.6 deer/km2 in a high density stratum. Sightability was positively influenced by the presence of white color-phase deer in a group and group size, and varied between airial and ground-based observers and by density strata. Our findings underscore the utility of double-observer surveys and aerial surveys with individually marked deer, both incorporating covariates to model sightability, to estimate deer abundance.

  5. Blending zone determination for aerial orthimage mosaicking

    NASA Astrophysics Data System (ADS)

    Lin, Chao-Hung; Chen, Bo-Heng; Lin, Bo-Yi; Chou, Han-Szu

    2016-09-01

    Creating a composed image from a set of aerial images is a fundamental step in orthomosaic generation. One of the processes involved in this technique is determining an optimal seamline in an overlapping region to stitch image patches seamlessly. Most previous studies have solved this optimization problem by searching for a one-pixel-wide seamline with an objective function. This strategy significantly reduced pixel mismatches on the seamline caused by geometric distortions of images but did not fully consider color discontinuity and mismatch problems that occur around the seamline, which sometimes cause mosaicking artifacts. This study proposes a blending zone determination scheme with a novel path finding algorithm to reduce the occurrence of unwanted artifacts. Instead of searching for a one-pixel-wide seamline, a blending zone, which is a k-pixel-wide seamline that passes through high-similarity pixels in the overlapping region, is determined using a hierarchical structure. This strategy allows for not only seamless stitching but also smooth color blending of neighboring image patches. Moreover, the proposed method searches for a blending zone without the pre-process of highly mismatched pixel removal and additional geographic data of road vectors and digital surface/elevation models, which increases the usability of the approach. Qualitative and quantitative analyses of aerial images demonstrate the superiority of the proposed method to related methods in terms of avoidance of passing highly mismatched pixels.

  6. An algorithm for approximate rectification of digital aerial images

    Technology Transfer Automated Retrieval System (TEKTRAN)

    High-resolution aerial photography is one of the most valuable tools available for managing extensive landscapes. With recent advances in digital camera technology, computer hardware, and software, aerial photography is easier to collect, store, and transfer than ever before. Images can be automa...

  7. Monitoring and Assuring the Quality of Digital Aerial Data

    NASA Technical Reports Server (NTRS)

    Christopherson, Jon

    2007-01-01

    This viewgraph presentation explains the USGS plan for monitoring and assuring the quality of digital aerial data. The contents include: 1) History of USGS Aerial Imaging Involvement; 2) USGS Research and Results; 3) Outline of USGS Quality Assurance Plan; 4) Other areas of Interest; and 5) Summary

  8. Aerial radiological survey of Area 11, Nevada Test Site

    SciTech Connect

    1983-06-01

    An aerial radiological survey of Area 11's Plutonium Valley was conducted at the Nevada Test Site from 18 to 30 January 1982. Contour maps representing terrestrial exposure rates and soil concentrations of transuranics, /sup 235/U and /sup 137/Cs are presented on an aerial photograph. Inventories of the locale's transuranic and uranium activities are also included.

  9. Kite Aerial Photography (KAP) as a Tool for Field Teaching

    ERIC Educational Resources Information Center

    Sander, Lasse

    2014-01-01

    Kite aerial photography (KAP) is proposed as a creative tool for geography field teaching and as a medium to approach the complexity of readily available geodata. The method can be integrated as field experiment, surveying technique or group activity. The acquired aerial images can instantaneously be integrated in geographic information systems…

  10. AERIAL OF VISITORS INFORMATION CENTER [VIC] & ROCKET GARDEN EXHIBIT

    NASA Technical Reports Server (NTRS)

    1975-01-01

    AERIAL OF VISITORS INFORMATION CENTER [VIC] & ROCKET GARDEN EXHIBIT KSC-375C-0604.12 116-KSC-375C-604.12, P-20220, ARCHIVE-04465 Aerial view of Kennedy Space Center Visitors Information Center looking east-northeastward. New food services building under construction is visible at upper left.

  11. The remote characterization of vegetation using Unmanned Aerial Vehicle photography

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Unmanned Aerial Vehicles (UAVs) can fly in place of piloted aircraft to gather remote sensing information on vegetation characteristics. The type of sensors flown depends on the instrument payload capacity available, so that, depending on the specific UAV, it is possible to obtain video, aerial phot...

  12. 12. Photocopy of photograph (original negative located at Aerial Mapping ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Photocopy of photograph (original negative located at Aerial Mapping Company, Phoenix, Arizona, Negative No. 90046) Photographer unknown, March 28, 1990. DIMENSION-CONTROLLED AERIAL PHOTOGRAPHIC MAP. - Yuma Main Street Water Treatment Plant, Jones Street at foot of Main Street, Yuma, Yuma County, AZ

  13. 11. Photographic copy of aerial photograph dated ca. 1954; Photographer ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Photographic copy of aerial photograph dated ca. 1954; Photographer unknown; Original owned by Waterloo Courier, Waterloo, Iowa; AERIAL VIEW OF RATH COMPLEX, LOOKING WEST; BEEF KILLING BUILDING (149 AND LIVESTOCK HOLDING AREAS ARE AT LEFT CENTER; FERTILIZER PLANT/STORAGE BUILDINGS ARE AT BOTTOM OF PHOTO - Rath Packing Company, Sycamore Street between Elm & Eighteenth Streets, Waterloo, Black Hawk County, IA

  14. 12. Photographic copy of aerial photograph dated October 1988; Photographed ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Photographic copy of aerial photograph dated October 1988; Photographed by Aerial Services, Incorporated, Waterloo, Iowa; THE RATH COMPLEX FROM DIRECTLY OVERHEAD; THE PACKING PLANT BUILDINGS OCCUPY UPPER RIGHT QUADRANT OF PHOTO; 18TH STREET BRIDGE AT CENTER - Rath Packing Company, Sycamore Street between Elm & Eighteenth Streets, Waterloo, Black Hawk County, IA

  15. DETAIL VIEW OF AERIAL TRAM CABLE COUNTERWEIGHT SYSTEM, LOOKING DOWN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    DETAIL VIEW OF AERIAL TRAM CABLE COUNTERWEIGHT SYSTEM, LOOKING DOWN THROUGH THE LOWER TERMINAL FLOOR. TWO SUSPENDED ROCK FILLED WOODEN BOXES CAN BE SEEN AT BOTTOM. THE METAL FRAMEWORK WAS INSTALLED BY THE PARK SERVICE DURING THE AERIAL TRAM'S STABILIZATION IN THE 1983. - Keane Wonder Mine, Park Route 4 (Daylight Pass Cutoff), Death Valley Junction, Inyo County, CA

  16. Calculation and uses of the lithographic aerial image

    NASA Astrophysics Data System (ADS)

    Flagello, Donis G.; Smith, Daniel G.

    2012-09-01

    Beginning with the seminal Dill papers of 1975, the aerial image has been essential for understanding the process of microlithography. From the aerial image, we can predict the performance of a given lithographic process in terms of depth of focus, exposure latitude, etc. As lithographic technologies improved, reaching smaller and smaller printed features, the sophistication of aerial image calculations has had to increase from simple incoherent imaging theory, to partial coherence, polarization effects, thin film effects at the resist, thick mask effects, and so on. This tutorial provides an overview and semihistorical development of the aerial image calculation and then provides a review of some of the various ways in which the aerial image is typically used to estimate the performance of the lithographic process.

  17. Observing snow cover using unmanned aerial vehicle

    NASA Astrophysics Data System (ADS)

    Spallek, Waldemar; Witek, Matylda; Niedzielski, Tomasz

    2016-04-01

    Snow cover is a key environmental variable that influences high flow events driven by snow-melt episodes. Estimates of snow extent (SE), snow depth (SD) and snow water equivalent (SWE) allow to approximate runoff caused by snow-melt episodes. These variables are purely spatial characteristics, and hence their pointwise measurements using terrestrial monitoring systems do not offer the comprehensive and fully-spatial information on water storage in snow. Existing satellite observations of snow reveal moderate spatial resolution which, not uncommonly, is not fine enough to estimate the above-mentioned snow-related variables for small catchments. High-resolution aerial photographs and the resulting orthophotomaps and digital surface models (DSMs), obtained using unmanned aerial vehicles (UAVs), may offer spatial resolution of 3 cm/px. The UAV-based observation of snow cover may be done using the near-infrared (NIR) cameras and visible-light cameras. Since the beginning of 2015, in frame of the research project no. LIDER/012/223/L-5/13/NCBR/2014 financed by the National Centre for Research and Development of Poland, we have performed a series of the UAV flights targeted at four sites in the Kwisa catchment in the Izerskie Mts. (part of the Sudetes, SW Poland). Observations are carried out with the ultralight UAV swinglet CAM (produced by senseFly, lightweight 0.5 kg, wingspan 80 cm) which enables on-demand sampling at low costs. The aim of the field work is to acquire aerial photographs taken using the visible-light and NIR cameras for a purpose of producing time series of DSMs and orthophotomaps with snow cover for all sites. The DSMs are used to calculate SD as difference between observational (with snow) and reference (without snow) models. In order to verify such an approach to compute SD we apply several procedures, one of which is the estimation of SE using the corresponding orthophotomaps generated on a basis of visual-light and NIR images. The objective of this

  18. Aerial monitoring and environmental protection: aerial photography as an instrument for checking landscape damage

    NASA Astrophysics Data System (ADS)

    Tartara, Patrizia

    2009-09-01

    C.N.R. and University of Salento have realized a Geographical Information System for heritage management of the national territory (landscape) and historical urban settlements. Informations come from bibliography, archives, direct and systematic field survey, different kind of aerial photographs analysis, with the primary aim of knowledge for the establishment of an in existence Cultural Heritage Cadastre, focused to legal protection and exploitation of the sites, not last the correct territory planning.

  19. Precision wildlife monitoring using unmanned aerial vehicles

    PubMed Central

    Hodgson, Jarrod C.; Baylis, Shane M.; Mott, Rowan; Herrod, Ashley; Clarke, Rohan H.

    2016-01-01

    Unmanned aerial vehicles (UAVs) represent a new frontier in environmental research. Their use has the potential to revolutionise the field if they prove capable of improving data quality or the ease with which data are collected beyond traditional methods. We apply UAV technology to wildlife monitoring in tropical and polar environments and demonstrate that UAV-derived counts of colony nesting birds are an order of magnitude more precise than traditional ground counts. The increased count precision afforded by UAVs, along with their ability to survey hard-to-reach populations and places, will likely drive many wildlife monitoring projects that rely on population counts to transition from traditional methods to UAV technology. Careful consideration will be required to ensure the coherence of historic data sets with new UAV-derived data and we propose a method for determining the number of duplicated (concurrent UAV and ground counts) sampling points needed to achieve data compatibility. PMID:26986721

  20. Precision wildlife monitoring using unmanned aerial vehicles.

    PubMed

    Hodgson, Jarrod C; Baylis, Shane M; Mott, Rowan; Herrod, Ashley; Clarke, Rohan H

    2016-03-17

    Unmanned aerial vehicles (UAVs) represent a new frontier in environmental research. Their use has the potential to revolutionise the field if they prove capable of improving data quality or the ease with which data are collected beyond traditional methods. We apply UAV technology to wildlife monitoring in tropical and polar environments and demonstrate that UAV-derived counts of colony nesting birds are an order of magnitude more precise than traditional ground counts. The increased count precision afforded by UAVs, along with their ability to survey hard-to-reach populations and places, will likely drive many wildlife monitoring projects that rely on population counts to transition from traditional methods to UAV technology. Careful consideration will be required to ensure the coherence of historic data sets with new UAV-derived data and we propose a method for determining the number of duplicated (concurrent UAV and ground counts) sampling points needed to achieve data compatibility.

  1. Bioinspired optical sensors for unmanned aerial systems

    NASA Astrophysics Data System (ADS)

    Chahl, Javaan; Rosser, Kent; Mizutani, Akiko

    2011-04-01

    Insects are dependant on the spatial, spectral and temporal distributions of light in the environment for flight control and navigation. This paper reports on flight trials of implementations of insect inspired behaviors on unmanned aerial vehicles. Optical flow methods for maintaining a constant height above ground and a constant course have been demonstrated to provide navigation capabilities that are impossible using conventional avionics sensors. Precision control of height above ground and ground course were achieved over long distances. Other vision based techniques demonstrated include a biomimetic stabilization sensor that uses the ultraviolet and green bands of the spectrum, and a sky polarization compass. Both of these sensors were tested over long trajectories in different directions, in each case showing performance similar to low cost inertial heading and attitude systems. The behaviors demonstrate some of the core functionality found in the lower levels of the sensorimotor system of flying insects and shows promise for more integrated solutions in the future.

  2. Global aerial flyways allow efficient travelling.

    PubMed

    Kranstauber, B; Weinzierl, R; Wikelski, M; Safi, K

    2015-12-01

    Birds migrate over vast distances at substantial costs. The highly dynamic nature of the air makes the selection of the best travel route difficult. We investigated to what extent migratory birds may optimise migratory route choice with respect to wind, and if route choice can be subject to natural selection. Following the optimal route, calculated using 21 years of empirical global wind data, reduced median travel time by 26.5% compared to the spatially shortest route. When we used a time-dependent survival model to quantify the adaptive benefit of choosing a fixed wind-optimised route, 84.8% of pairs of locations yielded a route with a higher survival than the shortest route. This suggests that birds, even if incapable of predicting wind individually, could adjust their migratory routes at a population level. As a consequence, this may result in the emergence of low-cost flyways representing a global network of aerial migratory pathways.

  3. Precision wildlife monitoring using unmanned aerial vehicles.

    PubMed

    Hodgson, Jarrod C; Baylis, Shane M; Mott, Rowan; Herrod, Ashley; Clarke, Rohan H

    2016-01-01

    Unmanned aerial vehicles (UAVs) represent a new frontier in environmental research. Their use has the potential to revolutionise the field if they prove capable of improving data quality or the ease with which data are collected beyond traditional methods. We apply UAV technology to wildlife monitoring in tropical and polar environments and demonstrate that UAV-derived counts of colony nesting birds are an order of magnitude more precise than traditional ground counts. The increased count precision afforded by UAVs, along with their ability to survey hard-to-reach populations and places, will likely drive many wildlife monitoring projects that rely on population counts to transition from traditional methods to UAV technology. Careful consideration will be required to ensure the coherence of historic data sets with new UAV-derived data and we propose a method for determining the number of duplicated (concurrent UAV and ground counts) sampling points needed to achieve data compatibility. PMID:26986721

  4. A Primer on Autonomous Aerial Vehicle Design.

    PubMed

    Coppejans, Hugo H G; Myburgh, Herman C

    2015-12-02

    There is a large amount of research currently being done on autonomous micro-aerial vehicles (MAV), such as quadrotor helicopters or quadcopters. The ability to create a working autonomous MAV depends mainly on integrating a simultaneous localization and mapping (SLAM) solution with the rest of the system. This paper provides an introduction for creating an autonomous MAV for enclosed environments, aimed at students and professionals alike. The standard autonomous system and MAV automation are discussed, while we focus on the core concepts of SLAM systems and trajectory planning algorithms. The advantages and disadvantages of using remote processing are evaluated, and recommendations are made regarding the viability of on-board processing. Recommendations are made regarding best practices to serve as a guideline for aspirant MAV designers.

  5. A Primer on Autonomous Aerial Vehicle Design

    PubMed Central

    Coppejans, Hugo H. G.; Myburgh, Herman C.

    2015-01-01

    There is a large amount of research currently being done on autonomous micro-aerial vehicles (MAV), such as quadrotor helicopters or quadcopters. The ability to create a working autonomous MAV depends mainly on integrating a simultaneous localization and mapping (SLAM) solution with the rest of the system. This paper provides an introduction for creating an autonomous MAV for enclosed environments, aimed at students and professionals alike. The standard autonomous system and MAV automation are discussed, while we focus on the core concepts of SLAM systems and trajectory planning algorithms. The advantages and disadvantages of using remote processing are evaluated, and recommendations are made regarding the viability of on-board processing. Recommendations are made regarding best practices to serve as a guideline for aspirant MAV designers. PMID:26633410

  6. A Primer on Autonomous Aerial Vehicle Design.

    PubMed

    Coppejans, Hugo H G; Myburgh, Herman C

    2015-01-01

    There is a large amount of research currently being done on autonomous micro-aerial vehicles (MAV), such as quadrotor helicopters or quadcopters. The ability to create a working autonomous MAV depends mainly on integrating a simultaneous localization and mapping (SLAM) solution with the rest of the system. This paper provides an introduction for creating an autonomous MAV for enclosed environments, aimed at students and professionals alike. The standard autonomous system and MAV automation are discussed, while we focus on the core concepts of SLAM systems and trajectory planning algorithms. The advantages and disadvantages of using remote processing are evaluated, and recommendations are made regarding the viability of on-board processing. Recommendations are made regarding best practices to serve as a guideline for aspirant MAV designers. PMID:26633410

  7. Aerial view of Launch Complex 39

    NASA Technical Reports Server (NTRS)

    1998-01-01

    In this aerial view looking south can be seen Launch Complex (LC) 39 area, where assembly, checkout and launch of the Space Shuttle Orbiter and its External Tank and twin Solid Rocket Boosters take place. Central to the complex is the tallest building at the center, the Vehicle Assembly Building (VAB). To the immediate left, from top to bottom, are the Orbiter Processing Facility (OPF) High Bay 3 and new engine shop (north side), OPF Modular Office Building, Thermal Protection System Facility, and a crawler-transporter (to its left). In front of the VAB are OPF 1 and OPF 2. At right is the Processing Control Center. West of OPF 3 is the Mobile Launch Platform. In the upper left corner is Launch Pad B; at the far right is the turn basin, with the Press Site located just below it to the right.

  8. GlxA is a new structural member of the radical copper oxidase family and is required for glycan deposition at hyphal tips and morphogenesis of Streptomyces lividans.

    PubMed

    Chaplin, Amanda K; Petrus, Marloes L C; Mangiameli, Giulia; Hough, Michael A; Svistunenko, Dimitri A; Nicholls, Peter; Claessen, Dennis; Vijgenboom, Erik; Worrall, Jonathan A R

    2015-08-01

    Streptomyces lividans displays a distinct dependence on copper to fully initiate morphological development. Evidence has accumulated to implicate the participation of an extracytoplasmic cuproenzyme in morphogenesis. In the present study, we show that GlxA fulfils all criteria to be that cuproenzyme. GlxA is membrane associated and has an active site consisting of a mononuclear copper and a cross-linked Y-C cofactor. The domain organization of the tertiary structure defines GlxA as a new structural member of the mono-copper oxidase family, with copper co-ordination geometry similar to, but spectroscopically distinct from fungal galactose oxidase (Gox). EPR spectroscopy reveals that the oxidation of cupric GlxA generates a protein radical residing on the Y-C cross-link. A variety of canonical Gox substrates (including D-galactose) were tested but none were readily turned over by GlxA. A glxA null-mutant leads to loss of glycan accumulation at hyphal tips and consequently a drastically changed morphology both on solid substrates and in liquid-grown environments, a scenario similarly observed in the absence of the neighbouring glycan synthase CslA (cellulase synthase-like protein). In addition the glxA mutant has lost the stimulation of development by copper, supporting a model whereby the enzymatic action of GlxA on the glycan is required for development and morphology. From a biotechnology perspective, the open mycelium morphology observed with the glxA mutant in submerged culture has implications for use as an enzyme production host.

  9. Diagnosis of invasive candidiasis by enzyme-linked immunosorbent assay using the N-terminal fragment of Candida albicans hyphal wall protein 1

    PubMed Central

    Laín, Ana; Elguezabal, Natalia; Brena, Sonia; García-Ruiz, Juan Carlos; del Palacio, Amalia; Moragues, María D; Pontón, José

    2007-01-01

    Background The diagnosis of invasive candidiasis is difficult because there are no specific clinical manifestations of the disease and colonization and infection are difficult to distinguish. In the last decade, much effort has been made to develop reliable tests for rapid diagnosis of invasive candidiasis, but none of them have found widespread clinical use. Results Antibodies against a recombinant N-terminal fragment of the Candida albicans germ tube-specific antigen hyphal wall protein 1 (Hwp1) generated in Escherichia coli were detected by both immunoblotting and ELISA tests in a group of 36 hematological or Intensive Care Unit patients with invasive candidiasis and in a group of 45 control patients at high risk for the mycosis who did not have clinical or microbiological data to document invasive candidiasis. Results were compared with an immunofluorescence test to detect antibodies to C. albicans germ tubes (CAGT). The sensitivity, specificity, positive and negative predictive values of a diagnostic test based on the detection of antibodies against the N-terminal fragment of Hwp1 by immunoblotting were 27.8 %, 95.6 %, 83.3 % and 62.3 %, respectively. Detection of antibodies to the N-terminal fragment of Hwp1 by ELISA increased the sensitivity (88.9 %) and the negative predictive value (90.2 %) but slightly decreased the specificity (82.6 %) and positive predictive values (80 %). The kinetics of antibody response to the N-terminal fragment of Hwp1 by ELISA was very similar to that observed by detecting antibodies to CAGT. Conclusion An ELISA test to detect antibodies against a recombinant N-terminal fragment of the C. albicans germ tube cell wall antigen Hwp1 allows the diagnosis of invasive candidiasis with similar results to those obtained by detecting antibodies to CAGT but without the need of treating the sera to adsorb the antibodies against the cell wall surface of the blastospore. PMID:17448251

  10. Coastline Extraction from Aerial Images Based on Edge Detection

    NASA Astrophysics Data System (ADS)

    Paravolidakis, V.; Moirogiorgou, K.; Ragia, L.; Zervakis, M.; Synolakis, C.

    2016-06-01

    Nowadays coastline extraction and tracking of its changes become of high importance because of the climate change, global warming and rapid growth of human population. Coastal areas play a significant role for the economy of the entire region. In this paper we propose a new methodology for automatic extraction of the coastline using aerial images. A combination of a four step algorithm is used to extract the coastline in a robust and generalizable way. First, noise distortion is reduced in order to ameliorate the input data for the next processing steps. Then, the image is segmented into two regions, land and sea, through the application of a local threshold to create the binary image. The result is further processed by morphological operators with the aim that small objects are being eliminated and only the objects of interest are preserved. Finally, we perform edge detection and active contours fitting in order to extract and model the coastline. These algorithmic steps are illustrated through examples, which demonstrate the efficacy of the proposed methodology.

  11. Large aerial bursts: an important class of terrestrial accretionary events.

    PubMed

    Wasson, John T

    2003-01-01

    Large aerial bursts similar to the 1908 Tunguska bolide but much larger in magnitude have surely been responsible for many catastrophic events in the history of the Earth. Because aerial bursts produce shallow (or even negligible) craters, their existence is difficult to document in the geological record. Even aerial bursts as small as Tunguska deposit enough energy to melt approximately 1mm of dry soil. Silica-rich glass formed in such melts has the potential to survive in the soil for many Ma, thus a potential indicator of large aerial bursts is glass that was formed as thick regions within silicate melt sheets. The layered tektites from Southeast Asia and the Libyan desert glass may have formed by a combination of sedimentation and downslope flow of silicate melt heated by radiation from large aerial bursts. The alternative, formation of layered tektites as crater ejecta, cannot account for observations such as uniformly high 10Be contents, the orientation of the magnetic remanence field, and the absence of splash-form (e.g., teardrop or dumbbell) tektites in regions where layered tektites are common. The largest asteroids or comets make craters no matter what their strength. Recent reviews suggest that, for events in the energy range up to 10(19)-10(20) J (about two orders of magnitude larger than the Meteor Crater impact), aerial bursts are more likely than cratering events, and the layered tektites of Southeast Asia imply the existence of aerial bursts one to two orders of magnitude larger still. PMID:12809134

  12. Large Aerial Bursts: An Important Class of Terrestrial Accretionary Events

    NASA Astrophysics Data System (ADS)

    Wasson, John T.

    2003-01-01

    Large aerial bursts similar to the 1908 Tunguska bolide but much larger in magnitude have surely been responsible for many catastrophic events in the history of the Earth. Because aerial bursts produce shallow (or even negligible) craters, their existence is difficult to document in the geological record. Even aerial bursts as small as Tunguska deposit enough energy to melt ~1mm of dry soil. Silica-rich glass formed in such melts has the potential to survive in the soil for many Ma, thus a potential indicator of large aerial bursts is glass that was formed as thick regions within silicate melt sheets. The layered tektites from Southeast Asia and the Libyan desert glass may have formed by a combination of sedimentation and downslope flow of silicate melt heated by radiation from large aerial bursts. The alternative, formation of layered tektites as crater ejecta, cannot account for observations such as uniformly high 10Be contents, the orientation of the magnetic remanence field, and the absence of splash-form (e.g., teardrop or dumbbell) tektites in regions where layered tektites are common. The largest asteroids or comets make craters no matter what their strength. Recent reviews suggest that, for events in the energy range up to 1019-1020 J (about two orders of magnitude larger than the Meteor Crater impact), aerial bursts are more likely than cratering events, and the layered tektites of Southeast Asia imply the existence of aerial bursts one to two orders of magnitude larger still.

  13. Building and road detection from large aerial imagery

    NASA Astrophysics Data System (ADS)

    Saito, Shunta; Aoki, Yoshimitsu

    2015-02-01

    Building and road detection from aerial imagery has many applications in a wide range of areas including urban design, real-estate management, and disaster relief. The extracting buildings and roads from aerial imagery has been performed by human experts manually, so that it has been very costly and time-consuming process. Our goal is to develop a system for automatically detecting buildings and roads directly from aerial imagery. Many attempts at automatic aerial imagery interpretation have been proposed in remote sensing literature, but much of early works use local features to classify each pixel or segment to an object label, so that these kind of approach needs some prior knowledge on object appearance or class-conditional distribution of pixel values. Furthermore, some works also need a segmentation step as pre-processing. Therefore, we use Convolutional Neural Networks(CNN) to learn mapping from raw pixel values in aerial imagery to three object labels (buildings, roads, and others), in other words, we generate three-channel maps from raw aerial imagery input. We take a patch-based semantic segmentation approach, so we firstly divide large aerial imagery into small patches and then train the CNN with those patches and corresponding three-channel map patches. Finally, we evaluate our system on a large-scale road and building detection datasets that is publicly available.

  14. Concept options for the aerial survey of Titan

    NASA Astrophysics Data System (ADS)

    Dorrington, G. E.

    2011-01-01

    Various aerial platforms intended for long endurance survey of the Titan surface are presented. A few novel concepts are introduced, including a heated methane balloon and a balloon with a tethered wind turbine. All the concept options are predicted to have lower scientific payload fractions than the Huygens probe. It is concluded that the selection of the best aerial platform option depends on more accurate mass estimates and a clear decision on whether, or not, in situ surface composition measurements are required in conjunction with aerial remote sensing.

  15. Aerial image retargeting (AIR): achieving litho-friendly designs

    NASA Astrophysics Data System (ADS)

    Yehia Hamouda, Ayman; Word, James; Anis, Mohab; Karim, Karim S.

    2011-04-01

    In this work, we present a new technique to detect non-Litho-Friendly design areas based on their Aerial Image signature. The aerial image is calculated for the litho target (pre-OPC). This is followed by the fixing (retargeting) the design to achieve a litho friendly OPC target. This technique is applied and tested on 28 nm metal layer and shows a big improvement in the process window performance. For an optimized Aerial-Image-Retargeting (AIR) recipe is very computationally efficient and its runtime doesn't consume more than 1% of the OPC flow runtime.

  16. Assessment of forest plantations from low altitude aerial photography. [North Carolina coastal plains

    NASA Technical Reports Server (NTRS)

    Nelson, H. A.

    1977-01-01

    Vertical color, and color-infrared, aerial photography obtained from altitudes between 183 m and 915 m provide a cost effective method of determining tree survival and height growth in pine plantations on the North Carolina Coastal Plain. All interpretations were performed by professional forestry personnel from the original 70 mm color transparencies. Prompt assessment of tree survival is necessary if failed spots are to be successfully replanted. Counts of living trees made after the third growing season, and sometimes only two growing seasons after planting, are accurate enough to permit planning of replanting operations without extensive ground surveys.

  17. Effects of aluminium in acid streams on growth and sporulation of aquatic hyphomycetes.

    PubMed

    Chamier, A C; Tipping, E

    1997-01-01

    We investigated, by field and laboratory experiments, the effects of aluminium in an acid stream (pH 5.0) on the growth and sporulation of aquatic hyphomycete fungi which degrade organic litter. The stream water had monomeric aluminium (Al(m)) concentrations of 9.1-13.4 microm - fifty times higher than a nearby circumneutral stream. Alder leaves submersed in the stream accumulated Al, most of which was tightly bound. Growth rates of four species of aquatic hyphomycetes were altered by inclusion of Al(m) in the culture medium. On a polypectate substrate, and on low-phosphate medium with glucose, growth rates increased significantly. On a low-nutrient substrate of homogenized alder leaves, growth rates were inhibited by aluminium. The pattern of mycelial growth was found to be different on a polypectate medium including Al(m), compared with a control without aluminium. There was a significant increase in hyphal radial growth and a decrease in the hyphal growth unit. The effect resembled the growth of a starved fungal colony. Treatment with Al(m) decreased pectinase production by the four fungal species tested. The capacity of these species to sporulate was reduced by flooding culture plates with Al(m) solution. These deleterious metabolic effects were most severe in isolates taken from circumneutral streams and less marked, though significant, in species originating from acid streams. PMID:15093395

  18. A new flavonoid from the aerial part of Thalictrum atriplex.

    PubMed

    Guangyao, G; Sibao, C; Junshan, Y; Peigen, X

    2000-12-01

    A new flavone glycoside, identified as kaempferol 3-O-[3'"-acetyl-alpha-L-arabinopyranosyl-(1'"-6")]-beta-D-gluco pyranoside (1), has been isolated from the aerial part of Thalictrum atriplex. PMID:11077167

  19. 33. GENERAL HIGH ALTITUDE AERIAL VIEW OF COMPLEX AND GENERAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    33. GENERAL HIGH ALTITUDE AERIAL VIEW OF COMPLEX AND GENERAL SETTING. October 1982 - Mississippi River 9-Foot Channel Project, Lock & Dam No. 15, Upper Mississipi River (Arsenal Island), Rock Island, Rock Island County, IL

  20. 13. GENERAL HIGH ALTITUDE AERIAL VIEW OF COMPLEX AND GENERAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. GENERAL HIGH ALTITUDE AERIAL VIEW OF COMPLEX AND GENERAL SETTING. October 1982 - Mississippi River 9-Foot Channel Project, Lock & Dam No. 17, Upper Mississippi River, New Boston, Mercer County, IL

  1. Aerial photography summary record system - five years later.

    USGS Publications Warehouse

    Lauterborn, T.J.

    1980-01-01

    Describes the APSRS, an automated information system for conventional aerial photography projects, established after the formation of the National Cartographic Information Center in the US Geological Survey in 1974. -after Author

  2. Bureau of Aeronautics, October 16, 1943, Photograph #4875. AERIAL OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Bureau of Aeronautics, October 16, 1943, Photograph #4875. AERIAL OF ROOSEVELT BASE LOOKING EAST - Roosevelt Base, Bounded by Ocean Boulevard, Pennsylvania Avenue, Richardson Avenue, & Idaho Street, Long Beach, Los Angeles County, CA

  3. 1. AERIAL VIEW LOOKING SOUTHWEST (OLD HARVARD STREET BRIDGE AT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. AERIAL VIEW LOOKING SOUTHWEST (OLD HARVARD STREET BRIDGE AT LEFT, NEW BRIDGE AT RIGHT) - Old Harvard Street Bridge, Spanning Rock Creek at National Zoological Park, Washington, District of Columbia, DC

  4. 1. AERIAL VIEW OF THE PATH TRANSIT SYSTEM BRIDGE, LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. AERIAL VIEW OF THE PATH TRANSIT SYSTEM BRIDGE, LOOKING NORTHEAST. THE CONRAIL BRIDGE (HAER No. NJ-43) AND THE NEWARK TURNPIKE ARE VISIBLE IN THE BACKGROUND - Path Transit System Bridge, Spanning Hackensack River, Kearny, Hudson County, NJ

  5. 45. Aerial view of station in 1944, four years after ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    45. Aerial view of station in 1944, four years after automation and before construction of the parking lot.U.S. Coast Guard (USCG) Photo - Bodie Island Light Station, Off Highway 12, Nags Head, Dare County, NC

  6. AERIAL VIEW FACING EAST. VIEW OF FABRIC BUILDING, STRUCTURAL WAREHOUSE, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AERIAL VIEW FACING EAST. VIEW OF FABRIC BUILDING, STRUCTURAL WAREHOUSE, RAIL MILLS & OPEN HEARTH COMPLEX (RIGHT TO LEFT). CITY OF MONESSEN IN BACKGROUND. - Pittsburgh Steel Company, Monessen Works, Donner Avenue, Monessen, Westmoreland County, PA

  7. 2. Photocopy of aerial view of the museum, taken October ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Photocopy of aerial view of the museum, taken October 26, 1966. Original photo in possession of the Philadelphia Museum of Art. - Philadelphia Museum of Art, Benjamin Franklin Parkway, Philadelphia, Philadelphia County, PA

  8. 21. AERIAL VIEW, LOOKING NORTH UP MEETING STREET FROM THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. AERIAL VIEW, LOOKING NORTH UP MEETING STREET FROM THE INTERSECTION OF MEETING AND BROAD STREETS (FOUR CORNERS--ST. MICHAEL'S EPISCOPAL CHURCH, FIREPROOF BUILDING, COURTHOUSE, U.S. POST OFFICE). - City Plan of Charleston, Charleston, Charleston County, SC

  9. 80. PHOTOCOPY OF 1976 AERIAL PHOTO OF BULLFROG MINE. From ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    80. PHOTOCOPY OF 1976 AERIAL PHOTO OF BULLFROG MINE. From National Park Service Environmental Review and Analysis, Bullfrog Mine Plan of Operations, Death Valley Nat'l Monument (24 March 1976) - Bullfrog Mine, Rhyolite, Nye County, NV

  10. 81. PHOTOCOPY OF 1978 AERIAL PHOTO OF BULLFROG MINE. From ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    81. PHOTOCOPY OF 1978 AERIAL PHOTO OF BULLFROG MINE. From National Park Service Environmental Review and Analysis, BullfroG Mine Plan of Operations, Death Valley Nat'l Monument (24 August 1978) - Bullfrog Mine, Rhyolite, Nye County, NV

  11. Photocopy of photograph. AERIAL VIEW. Original photograph taken April 1957 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Photocopy of photograph. AERIAL VIEW. Original photograph taken April 1957 by Mr. Lewis, and on file at the Edison National Historic Site, negative number 0-998 - Thomas A. Edison Laboratories, Main Street & Lakeside Avenue, West Orange, Essex County, NJ

  12. 69. AERIAL VIEW OF EAST END OF PARKWAY, LOOKING WEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    69. AERIAL VIEW OF EAST END OF PARKWAY, LOOKING WEST TOWARD BRIDGE AND SIKORSKY HELICOPTER PLANT. - Merritt Parkway, Beginning in Greenwich & running 38 miles to Stratford, Greenwich, Fairfield County, CT

  13. 64. AERIAL VIEW OF EAST END OF PARKWAY, LOOKING WEST, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    64. AERIAL VIEW OF EAST END OF PARKWAY, LOOKING WEST, SIKORSKY HELICOPTER PLANT TO THE RIGHT. - Merritt Parkway, Beginning in Greenwich & running 38 miles to Stratford, Greenwich, Fairfield County, CT

  14. 7. Historic aerial photo of rocket engine test facility complex, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Historic aerial photo of rocket engine test facility complex, June 1962. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA GRC photo number C-60674. - Rocket Engine Testing Facility, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH

  15. 14. Photocopy of Photograph AERIAL VIEW TO SOUTHEAST, GENERAL VIEW ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Photocopy of Photograph AERIAL VIEW TO SOUTHEAST, GENERAL VIEW OF COMPLEX, 16 April 1973. - Cape Canaveral Air Station, Launch Complex 17, East end of Lighthouse Road, Cape Canaveral, Brevard County, FL

  16. 13. Photocopy of Photograph AERIAL VIEW TO NORTHWEST, GENERAL VIEW ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Photocopy of Photograph AERIAL VIEW TO NORTHWEST, GENERAL VIEW OF COMPLEX, 16 April 1973. - Cape Canaveral Air Station, Launch Complex 17, East end of Lighthouse Road, Cape Canaveral, Brevard County, FL

  17. AERIAL OVERVIEW, LOOKING NORTH, WITH FORMER TCIUS STEEL ORE MINE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AERIAL OVERVIEW, LOOKING NORTH, WITH FORMER TCI-US STEEL ORE MINE HEADQUARTERS (BOTTOM) AND SUPERINTENDENT'S AND FOREMAN HOUSING ALONG MINNESOTA AVENUE AT CREST OF RED MOUNTAIN (TOP LEFT). - Muscoda Red Ore Mining Community, Bessemer, Jefferson County, AL

  18. AERIAL VIEW, LOOKING EAST TOWARDS TCIUS STEEL, ENSLEY WORKS OPEN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AERIAL VIEW, LOOKING EAST TOWARDS TCI-US STEEL, ENSLEY WORKS OPEN HEARTH IN BACKGROUND. - Tennessee Coal & Iron Company, Ensley Works, West of residential & commercial districts, Birmingham, Jefferson County, AL

  19. AERIAL VIEW, LOOKING SOUTH WEST, BIRMINGPORT ROAD AND DON DRENNEN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AERIAL VIEW, LOOKING SOUTH WEST, BIRMINGPORT ROAD AND DON DRENNEN OVERPASS IN FOREGROUND, TCI-US STEEL ENSLEY WORKS OPEN HEARTH (RUINS) IN THE BACKGROUND. - Tennessee Coal & Iron Company, Ensley Works, West of residential & commercial districts, Birmingham, Jefferson County, AL

  20. 52. CLOSEUP AERIAL VIEW OF THE MERCURY CAPSULE SITTING ON ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    52. CLOSE-UP AERIAL VIEW OF THE MERCURY CAPSULE SITTING ON TOP OF THE REDSTONE ROCKET IN THE TEST STAND. - Marshall Space Flight Center, Redstone Rocket (Missile) Test Stand, Dodd Road, Huntsville, Madison County, AL

  1. DETAIL VIEW OF AERIAL TRAM SUPPORT TOWER TWO, WITH TOWERS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    DETAIL VIEW OF AERIAL TRAM SUPPORT TOWER TWO, WITH TOWERS THREE,FOUR, FIVE AND SIX IN DISTANCE, LOOKING NORTHEAST. - Keane Wonder Mine, Park Route 4 (Daylight Pass Cutoff), Death Valley Junction, Inyo County, CA

  2. 45. HISTORIC AERIAL VIEW LOOKING SOUTHWEST AT THE TEST STAND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    45. HISTORIC AERIAL VIEW LOOKING SOUTHWEST AT THE TEST STAND AND THE SURROUNDING ELECTRONICS AND EQUIPMENT TRAILERS. - Marshall Space Flight Center, Redstone Rocket (Missile) Test Stand, Dodd Road, Huntsville, Madison County, AL

  3. 1. NORTHWEST OBLIQUE AERIAL VIEW OF FORT DELAWARE AND PEA ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. NORTHWEST OBLIQUE AERIAL VIEW OF FORT DELAWARE AND PEA PATCH ISLAND. REMAINS OF SEA WALL VISIBLE IN FOREGROUND AND RIGHT OF IMAGE. - Fort Delaware, Sea Wall, Pea Patch Island, Delaware City, New Castle County, DE

  4. NORTHWEST OBLIQUE AERIAL VIEW OF FORT DELAWARE AND PEA PATCH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    NORTHWEST OBLIQUE AERIAL VIEW OF FORT DELAWARE AND PEA PATCH ISLAND. REMAINS OF SEA WALL VISIBLE IN FOREGROUND AND RIGHT OF IMAGE - Fort Delaware, Pea Patch Island, Delaware City, New Castle County, DE

  5. 30. Aerial view looking SE at Brooklyn Tower and the ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    30. Aerial view looking SE at Brooklyn Tower and the intersection of Water Street and Camden Plaza. Jet Lowe, photographer, 1982. - Brooklyn Bridge, Spanning East River between Park Row, Manhattan and Sands Street, Brooklyn, New York County, NY

  6. 14. AERIAL VIEW OF ENGINE DISPLAY INSIDE PASSENGER CAR SHOP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. AERIAL VIEW OF ENGINE DISPLAY INSIDE PASSENGER CAR SHOP (NOW A TRANSPORTATION MUSEUM) - Baltimore & Ohio Railroad, Mount Clare Passenger Car Shop, Southwest corner of Pratt & Poppleton Streets, Baltimore, Independent City, MD

  7. 50. AERIAL VIEW OF THE SOUTH END OF ALEXANDRIA LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    50. AERIAL VIEW OF THE SOUTH END OF ALEXANDRIA LOOKING NORTH. (WASHINGTON ST.) - George Washington Memorial Parkway, Along Potomac River from McLean to Mount Vernon, VA, Mount Vernon, Fairfax County, VA

  8. 67. AERIAL VIEW OF WATERFOWL SANCTUARY WITH INTERSTATE 395 LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    67. AERIAL VIEW OF WATERFOWL SANCTUARY WITH INTERSTATE 395 LOOKING NORTHEAST INTO WASHINGTON D.C. - George Washington Memorial Parkway, Along Potomac River from McLean to Mount Vernon, VA, Mount Vernon, Fairfax County, VA

  9. 4. AERIAL VIEW OF MT. VERNON TERMINUS, SOUTHERN TERMINUS OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. AERIAL VIEW OF MT. VERNON TERMINUS, SOUTHERN TERMINUS OF GEORGE WASHINGTON MEMORIAL PARKWAY (GWMP), LOOKING SOUTHEAST. - George Washington Memorial Parkway, Along Potomac River from McLean to Mount Vernon, VA, Mount Vernon, Fairfax County, VA

  10. 2. AERIAL VIEW OF THE WEST GROUNDS OF THE CAPITOL, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. AERIAL VIEW OF THE WEST GROUNDS OF THE CAPITOL, UNION PLAZA AND REFLECTING POOL AND THE BOTANIC GARDENS, LOOKING NORTH FROM OVER FIRST STREET, SW. - National Mall & Monument Grounds, Washington, District of Columbia, DC

  11. 262. Frank Deras Jr., Photographer June 1998 AERIAL VIEW OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    262. Frank Deras Jr., Photographer June 1998 AERIAL VIEW OF CANTILEVER TRUSS CANTILEVER ARM AND SUSPENDED SPAN, NORTH SIDE, FACING SOUTH. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

  12. HISTORIC IMAGE: AERIAL VIEW OF CEMETERY AND ITS ENVIRONS. PHOTOGRAPH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    HISTORIC IMAGE: AERIAL VIEW OF CEMETERY AND ITS ENVIRONS. PHOTOGRAPH 15 SEPTEMBER 1950. NCA HISTORY COLLECTION. - San Francisco National Cemetery, 1 Lincoln Boulevard, San Francisco, San Francisco County, CA

  13. 13. AERIAL OF FORT SHERIDAN LOOKING NORTH TOWARD THE WATER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. AERIAL OF FORT SHERIDAN LOOKING NORTH TOWARD THE WATER TOWER (Copy negative made from negative by TASO, U.S. Army, Fort Sheridan, Illinois). - Fort Sheridan, 25 miles Northeast of Chicago, on Lake Michigan, Lake Forest, Lake County, IL

  14. 38. DETAIL AERIAL VIEW LOOKING SOUTHEAST AT 45 DEGREE ANGLE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    38. DETAIL AERIAL VIEW LOOKING SOUTHEAST AT 45 DEGREE ANGLE SHOWING TOP VIEW OF 210' 9' LIFT SPAN - Central Railroad of New Jersey, Newark Bay Lift Bridge, Spanning Newark Bay, Newark, Essex County, NJ

  15. 39. DETAIL AERIAL VIEW LOOKING AT 210' 9' LIFT SPAN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    39. DETAIL AERIAL VIEW LOOKING AT 210' 9' LIFT SPAN TOWER SHEAVES SHOWING 1 SET WITH AND 1 SET WITHOUT SHEAVE HOODS - Central Railroad of New Jersey, Newark Bay Lift Bridge, Spanning Newark Bay, Newark, Essex County, NJ

  16. 2. AERIAL VIEW OF ROLLING LIFT BRIDGE. DORCHESTER AVENUE IN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. AERIAL VIEW OF ROLLING LIFT BRIDGE. DORCHESTER AVENUE IN BACKGROUND. SOUTH STATION VISIBLE AT TOP LEFT. - New York, New Haven & Hartford Railroad, Fort Point Channel Rolling Lift Bridge, Spanning Fort Point Channel, Boston, Suffolk County, MA

  17. Unmanned Aerial Vehicle Systems for Disaster Relief: Tornado Alley

    NASA Technical Reports Server (NTRS)

    DeBusk, Wesley M.

    2009-01-01

    Unmanned aerial vehicle systems are currently in limited use for public service missions worldwide. Development of civil unmanned technology in the United States currently lags behind military unmanned technology development in part because of unresolved regulatory and technological issues. Civil unmanned aerial vehicle systems have potential to augment disaster relief and emergency response efforts. Optimal design of aerial systems for such applications will lead to unmanned vehicles which provide maximum potentiality for relief and emergency response while accounting for public safety concerns and regulatory requirements. A case study is presented that demonstrates application of a civil unmanned system to a disaster relief mission with the intent on saving lives. The concept utilizes unmanned aircraft to obtain advanced warning and damage assessments for tornados and severe thunderstorms. Overview of a tornado watch mission architecture as well as commentary on risk, cost, need for, and design tradeoffs for unmanned aerial systems are provided.

  18. 74. AERIAL VIEW OF MEMORIAL BRIDGE AND MEMORIAL AVENUE LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    74. AERIAL VIEW OF MEMORIAL BRIDGE AND MEMORIAL AVENUE LOOKING EAST AT LINCOLN MEMORIAL. - George Washington Memorial Parkway, Along Potomac River from McLean to Mount Vernon, VA, Mount Vernon, Fairfax County, VA

  19. Meteorological influences on mass accountability of aerially applied sprays

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The deposition and drift of aerially applied crop protection materials is influenced by a number of factors including equpment setup and operational parameters, spray material characteristics, and meteorological effects. This work examines the meteorological influences that effect the ultimate fate...

  20. 56. AERIAL VIEW OF WIDE MEDIAN NEXT TO WASHINGTON SAILING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    56. AERIAL VIEW OF WIDE MEDIAN NEXT TO WASHINGTON SAILING MARINA LOOKING NORTH. - George Washington Memorial Parkway, Along Potomac River from McLean to Mount Vernon, VA, Mount Vernon, Fairfax County, VA

  1. 54. AERIAL VIEW OF WIDE MEDIAN JUST SOUTH OF WASHINGTON ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    54. AERIAL VIEW OF WIDE MEDIAN JUST SOUTH OF WASHINGTON SAILING MARINA LOOKING NORTH. - George Washington Memorial Parkway, Along Potomac River from McLean to Mount Vernon, VA, Mount Vernon, Fairfax County, VA

  2. 11. COPY OF 1970 AERIAL PHOTOGRAPH OF LORING AIR FORCE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. COPY OF 1970 AERIAL PHOTOGRAPH OF LORING AIR FORCE BASE. PHOTOGRAPH LOCATED AT AIR FORCE BASE CONVERSION AGENCY, LORING AIR FORCE BASE, MAINE. - Loring Air Force Base, Airfield, Central portion of base, Limestone, Aroostook County, ME

  3. AERIAL VIEW, LOOKING NORTH NORTHWEST, OF ARRASTRA GULCH, WITH SILVER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AERIAL VIEW, LOOKING NORTH NORTHWEST, OF ARRASTRA GULCH, WITH SILVER LAKE IN FOREGROUND. NOTE SILVER LAKE MINE AND MILL RUINS ON FAR SHORE. - Shenandoah-Dives Mill, 135 County Road 2, Silverton, San Juan County, CO

  4. AERIAL VIEW, LOOKING NORTH, OF SILVER LAKE. NOTE IOWA MINE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AERIAL VIEW, LOOKING NORTH, OF SILVER LAKE. NOTE IOWA MINE RUINS AT LEFT CENTER AND SILVER LAKE MINE RUINS BEYOND NORTHWEST SHORE. - Shenandoah-Dives Mill, 135 County Road 2, Silverton, San Juan County, CO

  5. 1. AERIAL VIEW FROM SE LOOKING NW, SHOWING BRIDGE AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. AERIAL VIEW FROM SE LOOKING NW, SHOWING BRIDGE AND SITE CONTEXT - Slates' Mill Bridge, Township Road 439 spanning South Branch of Tunkhannock Creek in Benton Township, Dalton, Lackawanna County, PA

  6. 5. AERIAL VIEW, LOOKING NORTH, OF BUILDING 371 AFTER CONSTRUCTION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. AERIAL VIEW, LOOKING NORTH, OF BUILDING 371 AFTER CONSTRUCTION WAS COMPLETED. (11/7/78) - Rocky Flats Plant, Plutonium Recovery Facility, Northwest portion of Rocky Flats Plant, Golden, Jefferson County, CO

  7. 2. AERIAL VIEW OF BRIDGE IN CONTEXT FROM SOUTHWEST. LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. AERIAL VIEW OF BRIDGE IN CONTEXT FROM SOUTHWEST. LOOKING NORTHEAST. - Rue Road Bridge, Rue Road, spanning Matchaponix Brook, .35 mile east of intersection with Route 613, Jamesburg, Middlesex County, NJ

  8. 1. AERIAL VIEW OF BRIDGE IN CONTEXT INCLUDING VICTORY CIRCLE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. AERIAL VIEW OF BRIDGE IN CONTEXT INCLUDING VICTORY CIRCLE FROM SOUTH. LOOKING NORTH. - Rue Road Bridge, Rue Road, spanning Matchaponix Brook, .35 mile east of intersection with Route 613, Jamesburg, Middlesex County, NJ

  9. AERIAL VIEW, LOOKING SOUTH, OF SHENANDOAHDIVES (MAYFLOWER) MINE PORTAL, IN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AERIAL VIEW, LOOKING SOUTH, OF SHENANDOAH-DIVES (MAYFLOWER) MINE PORTAL, IN SHADOW AT WIDENED END OF ROAD, THREE-EIGHTHS FROM THE BOTTOM. - Shenandoah-Dives Mill, 135 County Road 2, Silverton, San Juan County, CO

  10. 83. Frank Deras Jr., Photographer June 1998 CONTEXTUAL AERIAL VIEW ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    83. Frank Deras Jr., Photographer June 1998 CONTEXTUAL AERIAL VIEW OF EAST BAY CROSSING FROM YERBA BUENA ISLAND TO OAKLAND, NORTH SIDE, FACING EAST. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

  11. 89. Frank Deras Jr., Photographer June 1998 CONTEXTUAL AERIAL VIEW ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    89. Frank Deras Jr., Photographer June 1998 CONTEXTUAL AERIAL VIEW OF EAST BAY CROSSING FROM YERBA BUENA ISLAND TO OAKLAND, FACING NORTHEAST. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

  12. Data annotation of aerial reconnaissance imagery and exploitation

    NASA Astrophysics Data System (ADS)

    Wareberg, P. Gunnar; Prunes, V.; Scholes, Richard W.

    1995-09-01

    This paper reviews the use of LED recording head assemblies (RHAs) for film annotation in aerial reconnaissance cameras and discusses code matrix block readers (CMBRs). Annotation of video imagery is also covered.

  13. 3. AERIAL VIEW OF PIER, LOOKING SOUTHWEST, WITH INTERSECTION OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. AERIAL VIEW OF PIER, LOOKING SOUTHWEST, WITH INTERSECTION OF PACIFIC COAST HIGHWAY AND MAIN STREET IN FOREGROUND - Huntington Beach Municipal Pier, Pacific Coast Highway at Main Street, Huntington Beach, Orange County, CA

  14. 1. AERIAL VIEW OF BOTH ACTIVE AND INACTIVE FLUMES, TAKEN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. AERIAL VIEW OF BOTH ACTIVE AND INACTIVE FLUMES, TAKEN FROM EAST, NOTE STOCK SHELTERS IN BACKGROUND AND HAYSTACKS AND STORAGE IN FOREGROUND - Grant-Kohrs Ranch, Flumes, Highway 10, Deer Lodge, Powell County, MT

  15. East wall, showing rails of a halfton aerial gantry attached ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    East wall, showing rails of a half-ton aerial gantry attached to roof frame - Bureau of Mines Metallurgical Research Laboratory, Original Building, Date Street north of U.S. Highway 93, Boulder City, Clark County, NV

  16. 1. AERIAL VIEW, LOOKING EAST SHOWING PACKAGE FREIGHTER (VESSEL 54), ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. AERIAL VIEW, LOOKING EAST SHOWING PACKAGE FREIGHTER (VESSEL 54), BROKEN BOW OF VESSEL IN FOREGROUND Charles Wisniewski, photographer, January 1985 - Shooters Island, Ships Graveyard, Vessel No. 54, Newark Bay, Staten Island (subdivision), Richmond County, NY

  17. 1. AERIAL VIEW, LOOKING NORTH, SHOWING COVERED BARGE (VESSEL 37) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. AERIAL VIEW, LOOKING NORTH, SHOWING COVERED BARGE (VESSEL 37) IN CENTER OF PICTURE WITH FOUR HATCHES SHOWING IN SUPERSTRUCTURE Charles Wisniewski, photographer, January 1985 - Shooters Island, Ships Graveyard, Vessel No. 37, Newark Bay, Staten Island (subdivision), Richmond County, NY

  18. 2. AERIAL VIEW, LOOKING NORTHWEST, SHOWING TOP, SIDE, AND REAR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. AERIAL VIEW, LOOKING NORTHWEST, SHOWING TOP, SIDE, AND REAR VIEW OF VESSEL 37 SUPERSTRUCTURE Charles Wisniewski, photographer, January 1985 - Shooters Island, Ships Graveyard, Vessel No. 37, Newark Bay, Staten Island (subdivision), Richmond County, NY

  19. 2. AERIAL VIEW, LOOKING EAST, SHOWING STERN OF HULL IN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. AERIAL VIEW, LOOKING EAST, SHOWING STERN OF HULL IN FOREGROUND. TWO MASTS VISIBLE Charles Wisniewski, photographer, JanuAry 1985 - Shooters Island, Ships Graveyard, Vessel No. 54, Newark Bay, Staten Island (subdivision), Richmond County, NY

  20. 2. NORTH SIDE. MASTER AERIAL SWITCH ON LOWER RIGHT PORTION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. NORTH SIDE. MASTER AERIAL SWITCH ON LOWER RIGHT PORTION OF WALL. TRIPOD AND TENSION WEIGHTS AT LEFT. - Chollas Heights Naval Radio Transmitting Facility, Helix House, 6410 Zero Road, San Diego, San Diego County, CA

  1. DETAIL TOP VIEW OF AERIAL TRAMWAY DRIVE MECHANISM, LOOKING NORTHEAST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    DETAIL TOP VIEW OF AERIAL TRAMWAY DRIVE MECHANISM, LOOKING NORTHEAST. THE FRICTION BRAKING SYSTEM CAN BE SEEN IN SHADOW ABOVE THE LARGE CABLE WHEEL BELOW. - Keane Wonder Mine, Park Route 4 (Daylight Pass Cutoff), Death Valley Junction, Inyo County, CA

  2. 326. Clyde Sunderland, Photographer November 11, 1936 AERIAL VIEW OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    326. Clyde Sunderland, Photographer November 11, 1936 AERIAL VIEW OF OAKLAND APPROACH TO YERBA BUENA ISLAND UNDER CONSTRUCTION. - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

  3. AERIAL OVERVIEW, LOOKING WEST, WITH FORMER TCIUS STEEL WORKER HOUSES ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AERIAL OVERVIEW, LOOKING WEST, WITH FORMER TCI-US STEEL WORKER HOUSES ALONG AVENUES G, H, I AND J AND MORGAN ROAD (BOTTOM, RUNNING LEFT TO RIGHT). - Muscoda Red Ore Mining Community, Bessemer, Jefferson County, AL

  4. AERIAL VIEW LOOKING FURTHER SOUTH EAST, VILLAGE CREEK WATER TREATMENT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AERIAL VIEW LOOKING FURTHER SOUTH EAST, VILLAGE CREEK WATER TREATMENT PLANT ON RIGHT SIDE, ENSLEY IN BACKGROUND. - Birmingham Southern Railroad Yard, Thirty-fourth Street, Ensley, Jefferson County, AL

  5. Aerial overview of the Denver International Airport site, looking southwest ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Aerial overview of the Denver International Airport site, looking southwest - Denver International Airport Site, Between Fifty-sixth & 128th Avenues, Buckley Road & Box Elder Creek, Denver, Denver County, CO

  6. 33. VIEW TO SOUTHEAST. AERIAL VIEW OF AREA DURING FLOOD ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    33. VIEW TO SOUTHEAST. AERIAL VIEW OF AREA DURING FLOOD STAGE. GIANELLA BRIDGE AT UPPER RIGHT Photographer unknown, January 24, 1970 - Gianella Bridge, Spanning Sacramento River at State Highway 32, Hamilton City, Glenn County, CA

  7. 68. Aerial view of SAC command post, building 500, looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    68. Aerial view of SAC command post, building 500, looking northeast, spring, 1957 - Offutt Air Force Base, Strategic Air Command Headquarters & Command Center, Headquarters Building, 901 SAC Boulevard, Bellevue, Sarpy County, NE

  8. 14. AERIAL VIEW OF POOL AND STRUCTURES Photocopy of photocopy ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. AERIAL VIEW OF POOL AND STRUCTURES Photocopy of photocopy of 1931 rendering by Alexander, Becker and Schoeppe, architects and engineers - Glen Echo Park, Crystal Swimming Pool, 7300 McArthur Boulevard, Glen Echo, Montgomery County, MD

  9. 3. AERIAL VIEW OF THE MALL BETWEEN TWELFTH STREET AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. AERIAL VIEW OF THE MALL BETWEEN TWELFTH STREET AND THE WASHINGTON MONUMENT, LOOKING NORTH UP THE 14TH STREET AXIS FROM OVER THE WASHINGTON CHANNEL. - National Mall & Monument Grounds, Washington, District of Columbia, DC

  10. 63. Aerial view of SAC command post construction, looking west ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    63. Aerial view of SAC command post construction, looking west - Offutt Air Force Base, Strategic Air Command Headquarters & Command Center, Headquarters Building, 901 SAC Boulevard, Bellevue, Sarpy County, NE

  11. 67. Aerial view of SAC command post, building 500, looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    67. Aerial view of SAC command post, building 500, looking northeast, undated - Offutt Air Force Base, Strategic Air Command Headquarters & Command Center, Headquarters Building, 901 SAC Boulevard, Bellevue, Sarpy County, NE

  12. 62. Aerial view of SAC command post, building 500, looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    62. Aerial view of SAC command post, building 500, looking east - Offutt Air Force Base, Strategic Air Command Headquarters & Command Center, Headquarters Building, 901 SAC Boulevard, Bellevue, Sarpy County, NE

  13. 25. AERIAL VIEW, LOOKING WEST OVER THE INTERSECTION OF MEETING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    25. AERIAL VIEW, LOOKING WEST OVER THE INTERSECTION OF MEETING AND TRADD STREETS WITH THE NATHANIEL RUSSELL HOUSE AND THE FIRST SCOTT PRESBYTERIAN CHURCH AS LANDMARKS ALONG MEETING STREET. - City Plan of Charleston, Charleston, Charleston County, SC

  14. Ontogeny of aerial righting and wing flapping in juvenile birds.

    PubMed

    Evangelista, Dennis; Cam, Sharlene; Huynh, Tony; Krivitskiy, Igor; Dudley, Robert

    2014-08-01

    Mechanisms of aerial righting in juvenile chukar partridge (Alectoris chukar) were studied from hatching to 14 days-post-hatching (dph). Asymmetric movements of the wings were used from 1 to 8 dph to effect progressively more successful righting behaviour via body roll. Following 8 dph, wing motions transitioned to bilaterally symmetric flapping that yielded aerial righting via nose-down pitch, along with substantial increases in vertical force production during descent. Ontogenetically, the use of such wing motions to effect aerial righting precedes both symmetric flapping and a previously documented behaviour in chukar (i.e. wing-assisted incline running) hypothesized to be relevant to incipient flight evolution in birds. These findings highlight the importance of asymmetric wing activation and controlled aerial manoeuvres during bird development and are potentially relevant to understanding the origins of avian flight. PMID:25165451

  15. Aerial view looking northwest. Buildings 1 and 2 fronting seaplane ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Aerial view looking northwest. Buildings 1 and 2 fronting seaplane ramps 2,3, and 4, are located bayside in center of view. - Naval Air Station North Island, North Island, San Diego, San Diego County, CA

  16. Historic Image: Aerial view of Mount of Victory Plot. Photograph ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Historic Image: Aerial view of Mount of Victory Plot. Photograph 1961. NCA History Collection - Cypress Hills National Cemetery, Mount of Victory Plot Unit, 625 Jamaica Avenue, Brooklyn, Kings County, NY

  17. 3. AERIAL VIEW SHOWING THE ENTIRE BRIDGE FROM EAST CABLE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. AERIAL VIEW SHOWING THE ENTIRE BRIDGE FROM EAST CABLE ANCHORAGE (EXTREME LEFT) TO WEST CABLE ANCHORAGE (UPPER RIGHT CORNER). March 1987. - Verde River Sheep Bridge, Spanning Verde River (Tonto National Forest), Cave Creek, Maricopa County, AZ

  18. AERIAL VIEW, LOOKING NORTH NORTHWEST, OF ARRASTRA BASIN, SILVER LAKE, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AERIAL VIEW, LOOKING NORTH NORTHWEST, OF ARRASTRA BASIN, SILVER LAKE, AND THE SHENANDOAH-DIVES (MAYFLOWER) MILL IN DISTANT ANIMAS VALLEY. - Shenandoah-Dives Mill, 135 County Road 2, Silverton, San Juan County, CO

  19. 1. AERIAL VIEW, SHOWING GLENDALE ROAD BRIDGE WITHIN ITS SETTING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. AERIAL VIEW, SHOWING GLENDALE ROAD BRIDGE WITHIN ITS SETTING AT GLENDALE ROAD CROSSING OF DEEP CREEK LAKE (PHOTOGRAPH BY RUTHVAN MORROW) - Glendale Road Bridge, Spanning Deep Creek Lake on Glendale Road, McHenry, Garrett County, MD

  20. 2. AERIAL VIEW, SHOWING GLENDALE ROAD BRIDGE WITHIN ITS SETTING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. AERIAL VIEW, SHOWING GLENDALE ROAD BRIDGE WITHIN ITS SETTING AT GLENDALE ROAD CROSSING OF DEEP CREEK LAKE (PHOTOGRAPH BY RUTHVAN MORROW) - Glendale Road Bridge, Spanning Deep Creek Lake on Glendale Road, McHenry, Garrett County, MD

  1. 1. AERIAL VIEW, SHOWING MOBILE LAUNCHER. BASE IS CALLED LAUNCH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. AERIAL VIEW, SHOWING MOBILE LAUNCHER. BASE IS CALLED LAUNCH PLATFORM AND TOWER ON RIGHT IS CALLED LAUNCH UMBILICAL TOWER, (LUT). - Mobile Launcher One, Kennedy Space Center, Titusville, Brevard County, FL

  2. 70. AERIAL VIEW OF ROUTE 110 WITH PENTAGON AND PARKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    70. AERIAL VIEW OF ROUTE 110 WITH PENTAGON AND PARKING AREA LOOKING SOUTHEAST.(EXPRESSWAY V.S. PARKWAY) - George Washington Memorial Parkway, Along Potomac River from McLean to Mount Vernon, VA, Mount Vernon, Fairfax County, VA

  3. Precision aerial application for site-specific rice crop management

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Precision agriculture includes different technologies that allow agricultural professional to use information management tools to optimize agriculture production. The new technologies allow aerial application applicators to improve application accuracy and efficiency, which saves time and money for...

  4. 3. AERIAL VIEW OF THREE BEARS LAKE, SHOWING OUTLET STREAM, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. AERIAL VIEW OF THREE BEARS LAKE, SHOWING OUTLET STREAM, BURLINGTON NORTHERN TRACKS, AND U.S. HIGHWAY 2, LOOKING NORTHEAST - Three Bears Lake & Dams, North of Marias Pass, East Glacier Park, Glacier County, MT

  5. Photocopy of aerial photograph taken in 1959, showing Newark Airport. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Photocopy of aerial photograph taken in 1959, showing Newark Airport. Photographer unknown. Original photograph property of Continental Airlines, Houston, Texas - Newark International Airport, Between New Jersey Turnpike, U.S. Routes 1 & 9, & Interstate 78, Newark, Essex County, NJ

  6. 1. AERIAL VIEW, LOOKING NORTH, FROM RED MOUNTAIN TO USX ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. AERIAL VIEW, LOOKING NORTH, FROM RED MOUNTAIN TO USX FAIRFIELD WORKS (TOP LEFT) WITH WENONAH SINTERING PLANT (BOTTOM CENTER) AND WENONAH COMMUNITY (CENTER RIGHT). - High Line Railroad, From Red Mountain to Fairfield Works, Birmingham, Jefferson County, AL

  7. 7 CFR 1755.507 - Aerial cable services.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., Design of Aerial Plant. The same tension as would be used in normal line construction so as not to exceed... masonry or on studs of wood frame buildings. Cable attachment devices may be installed on sheet...

  8. 7 CFR 1755.507 - Aerial cable services.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., Design of Aerial Plant. The same tension as would be used in normal line construction so as not to exceed... masonry or on studs of wood frame buildings. Cable attachment devices may be installed on sheet...

  9. 7 CFR 1755.507 - Aerial cable services.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., Design of Aerial Plant. The same tension as would be used in normal line construction so as not to exceed... masonry or on studs of wood frame buildings. Cable attachment devices may be installed on sheet...

  10. 7 CFR 1755.507 - Aerial cable services.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., Design of Aerial Plant. The same tension as would be used in normal line construction so as not to exceed... masonry or on studs of wood frame buildings. Cable attachment devices may be installed on sheet...

  11. 7 CFR 1755.507 - Aerial cable services.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., Design of Aerial Plant. The same tension as would be used in normal line construction so as not to exceed... masonry or on studs of wood frame buildings. Cable attachment devices may be installed on sheet...

  12. 1. AERIAL VIEW OF WHITSETT (INTAKE) PUMP PLANT ON LAKE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. AERIAL VIEW OF WHITSETT (INTAKE) PUMP PLANT ON LAKE SHORE IN FOREGROUND; GENE IN BACKGROUND, LOOKING SOUTHWEST. - Whitsett Pump Plant, West side of Colorado River, north of Parker Dam, Parker Dam, San Bernardino County, CA

  13. 4. AERIAL VIEW OF GENE WASH RESERVOIR AND GENE CAMP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. AERIAL VIEW OF GENE WASH RESERVOIR AND GENE CAMP LOOKING SOUTHWEST. DAM AND SPILLWAY VISIBLE IN BOTTOM OF PHOTO. - Gene Wash Reservoir & Dam, 2 miles west of Parker Dam, Parker Dam, San Bernardino County, CA

  14. 4. Aerial view of Whitsett intake (lower right), Parker Dam ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Aerial view of Whitsett intake (lower right), Parker Dam and village (left), Gene Wash Reservoir, Gene Pump Plant and village (right). - Parker Dam, Spanning Colorado River between AZ & CA, Parker, La Paz County, AZ

  15. 5. AERIAL VIEW LOOKING NORTH SHOWING PHILADELPHIA MUSEUM OF ART, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. AERIAL VIEW LOOKING NORTH SHOWING PHILADELPHIA MUSEUM OF ART, SITE OF FORMER MAIN STORAGE RESERVOIR - Fairmount Waterworks, East bank of Schuylkill River, Aquarium Drive, Philadelphia, Philadelphia County, PA

  16. 1. AERIAL VIEW LOOKING SOUTHEAST, SHOWING WATER WORKS SITE AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. AERIAL VIEW LOOKING SOUTHEAST, SHOWING WATER WORKS SITE AND PHILADELPHIA MUSEUM OF ART ON HILL ABOVE - Fairmount Waterworks, East bank of Schuylkill River, Aquarium Drive, Philadelphia, Philadelphia County, PA

  17. 2. AERIAL VIEW, LOOKING SW. TIP OF GOOSE ISLAND AT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. AERIAL VIEW, LOOKING SW. TIP OF GOOSE ISLAND AT TOP LEFT OF FRAME. - Chicago, Milwaukee & St. Paul Railway, Bridge No. Z-2, Spanning North Branch Canal at North Cherry Avenue, Chicago, Cook County, IL

  18. 3. AERIAL VIEW, LOOKING ESE. TIP OF GOOSE ISLAND AT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. AERIAL VIEW, LOOKING ESE. TIP OF GOOSE ISLAND AT TOP RIGHT OF FRAME. - Chicago, Milwaukee & St. Paul Railway, Bridge No. Z-2, Spanning North Branch Canal at North Cherry Avenue, Chicago, Cook County, IL

  19. 16. AERIAL VIEW OF GOOSE ISLAND, LOOKING SOUTH, CIRCA 1960. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. AERIAL VIEW OF GOOSE ISLAND, LOOKING SOUTH, CIRCA 1960. BRIDGE No. Z-2 AT LOWER LEFT OF FRAME. - Chicago, Milwaukee & St. Paul Railway, Bridge No. Z-2, Spanning North Branch Canal at North Cherry Avenue, Chicago, Cook County, IL

  20. 4. AERIAL VIEW OF DAM SITE SHOWING OUTLET WORKS AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. AERIAL VIEW OF DAM SITE SHOWING OUTLET WORKS AND DIVERSION CHANNEL IN FOREGROUND.... Volume XVIII, No. 9, March 5, 1940. - Prado Dam, Santa Ana River near junction of State Highways 71 & 91, Corona, Riverside County, CA

  1. 13. AERIAL VIEW SHOWING IN THE FOREGROUND, EXCAVATION FOR THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. AERIAL VIEW SHOWING IN THE FOREGROUND, EXCAVATION FOR THE SPILLWAY APRON.... Volume XVII, No. 12, December 26, 1939. - Prado Dam, Spillway, Santa Ana River near junction of State Highways 71 & 91, Corona, Riverside County, CA

  2. 6. EASTERLY AERIAL VIEW SHOWING THE RIGHT ABUTMENT AND OUTLET ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. EASTERLY AERIAL VIEW SHOWING THE RIGHT ABUTMENT AND OUTLET CONTROL WORKS IN THE FOREGROUND.... Volume XX, No. 8, September 9, 1940. - Prado Dam, Santa Ana River near junction of State Highways 71 & 91, Corona, Riverside County, CA

  3. 21. AERIAL VIEW OF THE OUTLET STRUCTURE AND OUTLET CHANNEL, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. AERIAL VIEW OF THE OUTLET STRUCTURE AND OUTLET CHANNEL, LOOKING UPSTREAM.... Volume XVII, No. 11, December 26, 1939. - Prado Dam, Outlet Works, Santa Ana River near junction of State Highways 71 & 91, Corona, Riverside County, CA

  4. 15. AERIAL PHOTOGRAPH OF DAM SITE SHOWING SPILLWAY OGEE SECTION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. AERIAL PHOTOGRAPH OF DAM SITE SHOWING SPILLWAY OGEE SECTION AND SPILLWAY APRON EXCAVATION IN FOREGROUND.... Volume XVIII, No. 10, January 18, 1940. - Prado Dam, Spillway, Santa Ana River near junction of State Highways 71 & 91, Corona, Riverside County, CA

  5. F/A-18 Automated Aerial Refueling (AAR) Phase 1

    NASA Video Gallery

    Engineers at NASA's Dryden Flight Research Center are evaluating the capability of an F/A-18A aircraft as an in-flight refueling tanker to develop analytical models for an automated aerial refuelin...

  6. 1. AERIAL SHOT HANGARS 14 (IN CENTER OF VIEW), WEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. AERIAL SHOT HANGARS 1-4 (IN CENTER OF VIEW), WEST FACING, SIDE LOOKING EAST. BUILDING 100 IS IN FOREGROUND. - Hill Field, Airplane Repair Hangars No. 1-No. 4, 5875 Southgate Avenue, Layton, Davis County, UT

  7. 2. AERIAL SHOT HANGARS 14 (IN CENTER OF VIEW), SOUTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. AERIAL SHOT HANGARS 1-4 (IN CENTER OF VIEW), SOUTH AND WEST FACING SIDES. BUILDING 238 IS IN LOWER RIGHT FOREGROUND. - Hill Field, Airplane Repair Hangars No. 1-No. 4, 5875 Southgate Avenue, Layton, Davis County, UT

  8. Aerial view of construction of both LTA ship hangars (looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Aerial view of construction of both LTA ship hangars (looking north) circa 1942. - Marine Corps Air Station Tustin, Northern Lighter Than Air Ship Hangar, Meffett Avenue & Maxfield Street, Tustin, Orange County, CA

  9. Aerial view of entire LTA base after completion of both ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Aerial view of entire LTA base after completion of both LTA ship hangars. Date unknown but probably circa 1945. - Marine Corps Air Station Tustin, Northern Lighter Than Air Ship Hangar, Meffett Avenue & Maxfield Street, Tustin, Orange County, CA

  10. Aerial view of reroofing of northern LTA ship hangar, circa ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Aerial view of re-roofing of northern LTA ship hangar, circa 1957. - Marine Corps Air Station Tustin, Northern Lighter Than Air Ship Hangar, Meffett Avenue & Maxfield Street, Tustin, Orange County, CA

  11. 129. FULL AERIAL VIEW SHOWING FORWARD PORT QUARTER, ENTERING PEARL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    129. FULL AERIAL VIEW SHOWING FORWARD PORT QUARTER, ENTERING PEARL HARBOR AFTER APOLLO 11 RECOVERY. 26 JULY 1969. (NATIONAL ARCHIVES NO. 428-KN-18090) - U.S.S. HORNET, Puget Sound Naval Shipyard, Sinclair Inlet, Bremerton, Kitsap County, WA

  12. 26. AERIAL VIEW OF WASTE CALCINING FACILITY WITH SOLIDS STORAGE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    26. AERIAL VIEW OF WASTE CALCINING FACILITY WITH SOLIDS STORAGE FACILITY BEHIND. CAMERA FACING EAST. INEEL PHOTO NUMBER PHOTO 72-4571. - Idaho National Engineering Laboratory, Old Waste Calcining Facility, Scoville, Butte County, ID

  13. 1. AERIAL VIEW SHOWING AQUEDUCT RIGHTOFWAY, WITH WASTE WEIR VISIBLE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. AERIAL VIEW SHOWING AQUEDUCT RIGHT-OF-WAY, WITH WASTE WEIR VISIBLE IN BACKGROUND. - Old Croton Aqueduct, Mill River Waste Weir, U.S. Route 9 at Sleepy Hollow Cemetery, Tarrytown, Westchester County, NY

  14. 25. CONSTRUCTION PROGRESS AERIAL VIEW OF WASTE CALCINING FACILITY TAKEN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    25. CONSTRUCTION PROGRESS AERIAL VIEW OF WASTE CALCINING FACILITY TAKEN WHEN STRUCTURE WAS 99 PERCENT COMPLETE. INEEL PHOTO NUMBER NRTS-60-5409. - Idaho National Engineering Laboratory, Old Waste Calcining Facility, Scoville, Butte County, ID

  15. 2. AERIAL VIEW LOOKING WEST SOUTHWEST SHOWING DOLPHIN MANUFACTURING CO., ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. AERIAL VIEW LOOKING WEST SOUTHWEST SHOWING DOLPHIN MANUFACTURING CO., BARBOUR FLAX SPINNING CO. -- SPRUCE ST. MILL, ROGERS LOCOMOTIVE AND MACHINE WORKS -- MILLWRIGHT SHOP AND FITTING SHOP. - Great Falls S. U. M. Historic District, Oliver Street, Paterson, Passaic County, NJ

  16. 10. AERIAL VIEW LOOKING SOUTH SOUTHEAST SHOWING (from left) INDUSTRY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. AERIAL VIEW LOOKING SOUTH SOUTHEAST SHOWING (from left) INDUSTRY MILL, HARMONY MILL, PHOENIX MILL, CONGDON MILL, TODD RAFFERTY MACHINE CO. - Great Falls S. U. M. Historic District, Oliver Street, Paterson, Passaic County, NJ

  17. 13. AERIAL VIEW LOOKING WEST SHOWING UNION WORKS (ROSEN MILL), ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. AERIAL VIEW LOOKING WEST SHOWING UNION WORKS (ROSEN MILL), GRANT LOCOMOTIVE WORKS -- MACHINE SHOP, DANFORTH (COOKE) LOCOMOTIVE AND MACHINE CO. - Great Falls S. U. M. Historic District, Oliver Street, Paterson, Passaic County, NJ

  18. 22. AERIAL VIEW LOOKING EAST SHOWING GRANT LOCOMOTIVE WORKS, UNION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. AERIAL VIEW LOOKING EAST SHOWING GRANT LOCOMOTIVE WORKS, UNION WORKS (ROSEN MILL), ROGERS LOCOMOTIVE AND MACHINE COMPANY AND IVANHOE MILL WHEELHOUSE UNDER RESTORATION. - Great Falls S. U. M. Historic District, Oliver Street, Paterson, Passaic County, NJ

  19. The Aeronautics Education, Research, and Industry Alliance (AERIAL) 2002 Report

    NASA Technical Reports Server (NTRS)

    Bowen, Brent D.; Fink, Mary M.; Nickerson, Jocelyn S.

    2002-01-01

    This report presents and overview of the Aeronautics Education, Research, and Industry Alliance (AERIAL). It covers the University of Nebraska's areas of research, and its outreach to students at Native American schools as part of AERIAL. The report contains three papers: "Airborne Remote Sensing (ARS) for Agricultural Research and Commercialization Application" (White Paper), "Validated Numerical Models for the Convective Extinction of Fuel Droplets (CEFD)", and "The Small Aircraft Transportation System (SATS): Research Collaborations with the NASA Langley Research Center".

  20. Optimization and application of Retinex algorithm in aerial image processing

    NASA Astrophysics Data System (ADS)

    Sun, Bo; He, Jun; Li, Hongyu

    2008-04-01

    In this paper, we provide a segmentation based Retinex for improving the visual quality of aerial images obtained under complex weather conditions. With the method, an aerial image will be segmented into different regions, and then an adaptive Gaussian based on the segmentations will be used to process it. The method addresses the problems existing in previously developed Retinex algorithms, such as halo artifacts and graying-out artifacts. The experimental result also shows evidence of its better effect.

  1. Ecological Energetics of an Abundant Aerial Insectivore, the Purple Martin

    PubMed Central

    Kelly, Jeffrey F.; Bridge, Eli S.; Frick, Winifred F.; Chilson, Phillip B.

    2013-01-01

    The atmospheric boundary layer and lower free atmosphere, or aerosphere, is increasingly important for human transportation, communication, environmental monitoring, and energy production. The impacts of anthropogenic encroachment into aerial habitats are not well understood. Insectivorous birds and bats are inherently valuable components of biodiversity and play an integral role in aerial trophic dynamics. Many of these insectivores are experiencing range-wide population declines. As a first step toward gaging the potential impacts of these declines on the aerosphere’s trophic system, estimates of the biomass and energy consumed by aerial insectivores are needed. We developed a suite of energetics models for one of the largest and most common avian aerial insectivores in North America, the Purple Martin (Prognesubis). The base model estimated that Purple Martins consumed 412 (± 104) billion insects*y-1 with a biomass of 115,860 (± 29,192) metric tonnes*y-1. During the breeding season Purple Martins consume 10.3 (+ 3.0) kg of prey biomass per km3 of aerial habitat, equal to about 36,000 individual insects*km-3. Based on these calculations, the cumulative seasonal consumption of insects*km-3 is greater in North America during the breeding season than during other phases of the annual cycle, however the maximum daily insect consumption*km-3 occurs during fall migration. This analysis provides the first range-wide quantitative estimate of the magnitude of the trophic impact of this large and common aerial insectivore. Future studies could use a similar modeling approach to estimate impacts of the entire guild of aerial insectivores at a variety of temporal and spatial scales. These analyses would inform our understanding of the impact of population declines among aerial insectivores on the aerosphere’s trophic dynamics. PMID:24086755

  2. User guide for the USGS aerial camera Report of Calibration.

    USGS Publications Warehouse

    Tayman, W.P.

    1984-01-01

    Calibration and testing of aerial mapping cameras includes the measurement of optical constants and the check for proper functioning of a number of complicated mechanical and electrical parts. For this purpose the US Geological Survey performs an operational type photographic calibration. This paper is not strictly a scientific paper but rather a 'user guide' to the USGS Report of Calibration of an aerial mapping camera for compliance with both Federal and State mapping specifications. -Author

  3. Systemic Administration of Human Bone Marrow-Derived Mesenchymal Stromal Cell Extracellular Vesicles Ameliorates Aspergillus Hyphal Extract-Induced Allergic Airway Inflammation in Immunocompetent Mice

    PubMed Central

    Cruz, Fernanda F.; Borg, Zachary D.; Goodwin, Meagan; Sokocevic, Dino; Wagner, Darcy E.; Coffey, Amy; Antunes, Mariana; Robinson, Kristen L.; Mitsialis, S. Alex; Kourembanas, Stella; Thane, Kristen; Hoffman, Andrew M.; McKenna, David H.; Rocco, Patricia R.M.

    2015-01-01

    An increasing number of studies demonstrate that administration of either conditioned media (CM) or extracellular vesicles (EVs) released by mesenchymal stromal cells (MSCs) derived from bone marrow and other sources are as effective as the MSCs themselves in mitigating inflammation and injury. The goal of the current study was to determine whether xenogeneic administration of CM or EVs from human bone marrow-derived MSCs would be effective in a model of mixed Th2/Th17, neutrophilic-mediated allergic airway inflammation, reflective of severe refractory asthma, induced by repeated mucosal exposure to Aspergillus hyphal extract (AHE) in immunocompetent C57Bl/6 mice. Systemic administration of both CM and EVs isolated from human and murine MSCs, but not human lung fibroblasts, at the onset of antigen challenge in previously sensitized mice significantly ameliorated the AHE-provoked increases in airway hyperreactivity (AHR), lung inflammation, and the antigen-specific CD4 T-cell Th2 and Th17 phenotype. Notably, both CM and EVs from human MSCs (hMSCs) were generally more potent than those from mouse MSCs (mMSCs) in most of the outcome measures. The weak cross-linking agent 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride was found to inhibit release of both soluble mediators and EVs, fully negating effects of systemically administered hMSCs but only partly inhibited the ameliorating effects of mMSCs. These results demonstrate potent xenogeneic effects of CM and EVs from hMSCs in an immunocompetent mouse model of allergic airway inflammation and they also show differences in mechanisms of action of hMSCs versus mMSCs to mitigate AHR and lung inflammation in this model. Significance There is a growing experience demonstrating benefit of mesenchymal stromal cell (MSC)-based cell therapies in preclinical models of asthma. In the current study, conditioned media (CM) and, in particular, the extracellular vesicle fraction obtained from the CM were as potent as the

  4. Diffusible component from the spore surface of the fungus Aspergillus fumigatus which inhibits the macrophage oxidative burst is distinct from gliotoxin and other hyphal toxins

    PubMed Central

    Mitchell, C. G.; Slight, J.; Donaldson, K.

    1997-01-01

    BACKGROUND: The fungus Aspergillus fumigatus, whose spores are present ubiquitously in the air, causes a range of diseases in the human lung. A small molecular weight (< 10 kD) heat stable toxin released from the spores of clinical and environmental isolates of A fumigatus within minutes of deposition in aqueous solution has previously been described. A key effect of the toxin was to inhibit the oxidative burst of macrophages as measured by superoxide anion release. It was hypothesised that the toxin was one of the commonly found A fumigatus hyphal toxins such as gliotoxin. This inhibitor may be an important factor which allows the fungus to colonise the lung. METHODS: The spore derived inhibitor was shown to inhibit the respiratory burst of rat alveolar macrophages, as measured by the generation of superoxide anion. Samples of the spore diffusate were subject to reversed phase high performance liquid chromatography (HPLC), thin layer chromatography (TLC), high performance thin layer chromatography (HPTLC), or organic extraction followed by TLC or HPLC to identify the presence of gliotoxin, fumagillin, helvolic acid, fumigaclavine-C, and aurasperone-C. Commercially obtained preparations of the toxins gliotoxin, fumagillin and helvolic acid and extracts enriched for fumigaclavine-C and aurasperone-C were used as internal and external standards and in the respiratory burst measurements. RESULTS: Gliotoxin, fumagillin, helvolic acid, fumigaclavine-C, and aurasperone- C were not detected in spore derived diffusate using PHLC or TLC. Using extraction procedures with solvents known to extract gliotoxin from A fumigatus culture supernatants, no gliotoxin was detected in the spore derived diffusate. Commercial gliotoxin, fumagillin, and helvolic acid or extracts enriched for fumigaclavine-C and aurasperone-C did not inhibit the oxidative burst of macrophages. CONCLUSIONS: The hypothesis that the spore derived toxin is one of the toxins derived from hyphae such as gliotoxin

  5. Measured Noise from Small Unmanned Aerial Vehicles

    NASA Technical Reports Server (NTRS)

    Cabell, Randolph; McSwain, Robert; Grosveld, Ferdinand

    2016-01-01

    Proposed uses of small unmanned aerial vehicles (UAVs), including home package delivery, have the potential to expose large portions of communities to a new noise source. This paper discusses results of flyover noise measurements of four small UAVs, including an internal combustion-powered model airplane and three battery-powered multicopters. Basic noise characteristics of these vehicles are discussed, including spectral properties and sound level metrics such as sound pressure level, effective perceived noise level, and sound exposure level. The size and aerodynamic characteristics of the multicopters in particular make their flight path susceptible to atmospheric disturbances such as wind gusts. These gusts, coupled with a flight control system that varies rotor speed to maintain vehicle stability, create an unsteady acoustic signature. The spectral variations resulting from this unsteadiness are explored, in both hover and flyover conditions for the multicopters. The time varying noise, which differs from the relatively steady noise generated by large transport aircraft, may complicate the prediction of human annoyance using conventional sound level metrics.

  6. Solar-powered unmanned aerial vehicles

    SciTech Connect

    Reinhardt, K.C.; Lamp, T.R.; Geis, J.W.; Colozza, A.J.

    1996-12-31

    An analysis was performed to determine the impact of various power system components and mission requirements on the size of solar-powered high altitude long endurance (HALE)-type aircraft. The HALE unmanned aerial vehicle (UAV) has good potential for use in many military and civil applications. The primary power system components considered in this study were photovoltaic (PV) modules for power generation and regenerative fuel cells for energy storage. The impact of relevant component performance on UAV size and capability were considered; including PV module efficiency and mass, power electronics efficiency, and fuel cell specific energy. Mission parameters such as time of year, flight altitude, flight latitude, and payload mass and power were also varied to determine impact on UAV size. The aircraft analysis method used determines the required aircraft wing aspect ratio, wing area, and total mass based on maximum endurance or minimum required power calculations. The results indicate that the capacity of the energy storage system employed, fuel cells in this analysis, greatly impacts aircraft size, whereas the impact of PV module efficiency and mass is much less important. It was concluded that an energy storage specific energy (total system) of 250--500 Whr/kg is required to enable most useful missions, and that PV cells with efficiencies greater than {approximately} 12% are suitable for use.

  7. The design of aerial camera focusing mechanism

    NASA Astrophysics Data System (ADS)

    Hu, Changchang; Yang, Hongtao; Niu, Haijun

    2015-10-01

    In order to ensure the imaging resolution of aerial camera and compensating defocusing caused by the changing of atmospheric temperature, pressure, oblique photographing distance and other environmental factor [1,2], and to meeting the overall design requirements of the camera for the lower mass and smaller size , the linear focusing mechanism is designed. Through the target surface support, the target surface component is connected with focusing driving mechanism. Make use of precision ball screws, focusing mechanism transforms the input rotary motion of motor into linear motion of the focal plane assembly. Then combined with the form of linear guide restraint movement, the magnetic encoder is adopted to detect the response of displacement. And the closed loop control is adopted to realize accurate focusing. This paper illustrated the design scheme for a focusing mechanism and analyzed its error sources. It has the advantages of light friction and simple transmission chain and reducing the transmission error effectively. And this paper also analyses the target surface by finite element analysis and lightweight design. Proving that the precision of focusing mechanism can achieve higher than 3um, and the focusing range is +/-2mm.

  8. [Death by explosion of an aerial mine].

    PubMed

    Stockhausen, Sarah; Wöllner, Kirsten; Madea, Burkhard; Doberentz, Elke

    2014-01-01

    Civilians are rarely killed by military weapons except in times of war. In early 2014, a 50-year-old man died in an explosion of an aerial mine from the Second World War when he was crushing concrete chunks with an excavator at a recycling plant. In the burned operator's cab, the remains of a body were found on the driver's seat. The thorax and the head were missing. Still sticking in the shoe, the right foot severed at the ankle was found about 7 m from the excavator together with numerous small to tiny body parts. At autopsy, the completely disrupted, strongly charred lower torso of a male connected to the left extremities as well as a large number of small tissue fragments and calcined bones were found. According to calculations performed by the seismographical station on the basis of seismic data, only about 45-60 percent of the charge had detonated. The autopsy results illustrate all the more the massive impact of such an explosion. PMID:26548019

  9. Directed aerial robot explorers for planetary exploration

    NASA Astrophysics Data System (ADS)

    Pankine, A. A.; Aaron, K. M.; Heun, M. K.; Nock, K. T.; Schlaifer, R. S.; Wyszkowski, C. J.; Ingersoll, A. P.; Lorenz, R. D.

    2004-01-01

    Global Aerospace Corporation (GAC) is developing a revolutionary system architecture for exploration of planetary atmospheres and surfaces from atmospheric altitudes. The work is supported by the NASA Institute for Advanced Concepts (NIAC). The innovative system architecture relies upon the use of Directed Aerial Robot Explorers (DAREs), which essentially are long-duration-flight autonomous balloons with trajectory control capabilities that can deploy swarms of miniature probes over multiple target areas. The balloons will serve a dual purpose as independent explorers and as microprobe delivery systems for targeted observations. Trajectory control capabilities will offer unprecedented opportunities in high-resolution, targeted observations of both atmospheric and surface phenomena. Multifunctional microprobes will be deployed from the balloons once over the target areas, and perform a multitude of functions, such as atmospheric profiling or surface exploration, relaying data back to the balloons or an orbiter. This architecture will enable low-cost, low-energy, long-term global exploration of planetary atmospheres and surfaces. We report here results of the preliminary analysis of the trajectory control capabilities and potential applications for DARE platforms at Venus, Mars, Titan and Jupiter.

  10. Mars Exploration with Directed Aerial Robot Explorers

    NASA Astrophysics Data System (ADS)

    Pankine, Alexey A.; Aaron, Kim M.; Heun, Matthew K.; Nock, Kerry T.; Schlaifer, R. Stephen; Ingersoll, Andrew P.; Lorenz, Ralph D.

    2004-02-01

    Global Aerospace Corporation (GAC) is developing a revolutionary system architecture for exploration of planetary atmospheres and surfaces from atmospheric altitudes. The work is supported by the NASA Institute for Advanced Concepts (NIAC). The innovative system architecture relies upon the use of Directed Aerial Robot Explorers (DAREs), which essentially are long-duration-flight autonomous balloons with trajectory control capabilities that can deploy swarms of miniature probes over multiple target areas. Balloon guidance capabilities will offer unprecedented opportunities in high-resolution, targeted observations of both atmospheric and surface phenomena. Multifunctional microprobes will be deployed from the balloons when over the target areas, and perform a multitude of functions, such as atmospheric profiling or surface exploration, relaying data back to the balloons or an orbiter. This architecture will enable low-cost, low-energy, long-term global exploration of planetary atmospheres and surfaces. A conceptual analysis of DARE capabilities and science applications for Mars is presented. Initial results of simulations indicate that a relatively small trajectory control wing can significantly change planetary balloon flight paths, especially during summer seasons in Polar Regions. This opens new possibilities for high-resolution observations of crustal magnetic anomalies, polar layered terrain, polar clouds, dust storms at the edges of the Polar caps and of seasonal variability of volatiles in the atmosphere.

  11. Adapting unmanned aerial vehicles for turbulence measurement

    NASA Astrophysics Data System (ADS)

    Witte, Brandon; Helvey, Jacob; Mullen, Jon; Thamann, Michael; Bailey, Sean

    2015-11-01

    We describe the approach of using highly instrumented and autonomous unmanned aerial vehicles (UAVs) to spatially interrogate the atmospheric boundary layer's turbulent flow structure. This approach introduces new capabilities not available in contemporary micro-meteorological measurement techniques such as instrumented towers, balloons, and manned aircraft. A key advantage in utilizing UAVs as an atmospheric turbulence research tool is that it reduces the reliance on assumptions regarding temporal evolution of the turbulence inherent within Taylor's frozen flow hypothesis by facilitating the ability to spatially sample the flow field over a wide range of spatial scales. In addition, UAVs offer the ability to measure in a wide range of boundary conditions and distance from the earth's surface, the ability to gather many boundary layer thicknesses of data during brief periods of statistical quasi-stationarity, and the ability to acquire data where and when it is needed. We describe recent progress made in manufacturing purpose-built airframes and adapting pre-fabricated airframes for these measurements by integrating sensors into those airframes and developing data analysis techniques to isolate the atmospheric turbulence from the measured velocity signal. This research is supported by NSF Award CBET-1351411.

  12. Aerial view of the KSC crawler transporters

    NASA Technical Reports Server (NTRS)

    1998-01-01

    In this aerial view the Crawler Transporter Maintenance Building (center) sits between two crawler transporters. The KSC crawlers are the largest tracked vehicles known. Once used to move assembled Apollo/Saturn from the VAB to the launch pad, they are now used for transporting Shuttle vehicles. They move the Mobile Launcher Platform into the Vehicle Assembly Building and then to the Launch Pad with an assembled space vehicle. Maximum speed is 1.6 km (one mile) per hour loaded, about 3.2 km (2 miles) per hour unloaded. Launch Pad to VAB trip time with the Mobile Launch Platform is about 5 hours. The crawler burns 568 liters (150 gallons) of diesel oil per mile. KSC's two crawlers have accumulated 1,243 miles since 1977. Including the Apollo years, the transporters have racked up 2,526 miles, about the same distance as a one-way trip from KSC to Los Angeles by interstate highway or a round trip between KSC and New York City.

  13. Unmanned Aerial Vehicle in Cadastral Applications

    NASA Astrophysics Data System (ADS)

    Manyoky, M.; Theiler, P.; Steudler, D.; Eisenbeiss, H.

    2011-09-01

    This paper presents the investigation of UAVs (Unmanned Aerial Vehicles) for use in cadastral surveying. Within the scope of a pilot study UAVs were tested for capturing geodata and compared with conventional data acquisition methods for cadastral surveying. Two study sites were therefore surveyed with a tachymeter-GNSS combination as well as a UAV system. The workflows of both methods were investigated and the resulting data were compared with the requirements of Swiss cadastral surveying. Concerning data acquisition and evaluation, the two systems are found to be comparable in terms of time expenditure, accuracy, and completeness. In conclusion, the UAV image orientation proved to be the limiting factor for the obtained accuracy due to the low- cost camera including camera calibration, image quality, and definition of the ground control points (natural or artificial). However, the required level of accuracy for cadastral surveying was reached. The advantage of UAV systems lies in their high flexibility and efficiency in capturing the surface of an area from a low flight altitude. In addition, further information such as orthoimages, elevation models and 3D objects can easily be gained from UAV images. Altogether, this project endorses the benefit of using UAVs in cadastral applications and the new opportunities they provide for cadastral surveying.

  14. Localization of aerial pure tones by pinnipeds

    NASA Astrophysics Data System (ADS)

    Holt, Marla M.; Schusterman, Ronald J.; Kastak, David; Southall, Brandon L.

    2005-12-01

    In this study, minimum audible angles (MAAs) of aerial pure tones were measured in and compared between a northern elephant seal (Mirounga angustirostris), a harbor seal (Phoca vitulina), and a California sea lion (Zalophus californianus). Testing was conducted between 0.8 and 16 kHz in the elephant seal and 0.8 and 20 kHz in the harbor seal and sea lion in a hemi-anechoic chamber using a left/right psychophysical procedure. Performance for the same frequencies was also quantified for discrete speaker separation of 5° from the mid-line. For all subjects, MAAs ranged from approximately 3° to 15° and were generally equal to or larger than those previously measured in the same subjects with a broadband signal. Performance at 5° ranged from chance to 97% correct, depending on frequency and subject. Poorest performance in the sea lion and harbor seal occurred at intermediate frequencies, which is consistent with the duplex theory of sound localization. In contrast, the elephant seal's poorest performance occurred at higher frequencies. The elephant seal's result suggests an inferior ability to utilize interaural level differences and is perhaps related to best hearing sensitivity shifted toward lower frequencies in this species relative to other pinnipeds.

  15. Pasadena, California Anaglyph with Aerial Photo Overlay

    NASA Technical Reports Server (NTRS)

    2000-01-01

    This anaglyph shows NASA's Jet Propulsion Laboratory (JPL) in Pasadena, California. Red-blue glasses are required to see the 3-D effect. The surrounding residential areas of La Canada-Flintridge (to the left) and Altadena/Pasadena (to the right) are also shown. JPL is located at the base of the San Gabriel Mountains, an actively growing mountain range, seen towards the top of the image. The large canyon coming out of the mountains (top to bottom of image) is the Arroyo Seco, which is a major drainage channel for the mountains. Sand and gravel removal operations in the lower part of the arroyo (bottom of image) are removing debris brought down by flood and mudflow events. Old landslide scars (lobe-shaped features) are seen in the arroyo, evidence that living near steep canyon slopes in tectonically active areas can be hazardous. The data can also be utilized by recreational users such as hikers enjoying the natural beauty of these rugged mountains.

    This anaglyph was generated using topographic data from the Shuttle Radar Topography Mission to create two differing perspectives of a single image, one perspective for each eye. The detailed aerial image was provided by U. S. Geological Survey digital orthophotography. Each point in the image is shifted slightly, depending on its elevation. When viewed through special glasses, the result is a vertically exaggerated view of the Earth's surface in its full three dimensions. Anaglyph glasses cover the left eye with a red filter and cover the right eye with a blue filter.

    The Shuttle Radar Topography Mission (SRTM), launched on February 11,2000, uses the same radar instrument that comprised the Spaceborne Imaging Radar-C/X-Band Synthetic Aperture Radar (SIR-C/X-SAR) that flew twice on the Space Shuttle Endeavour in 1994. The mission is designed to collect three-dimensional measurements of the Earth's surface. To collect the 3-D data, engineers added a 60-meter-long (200-foot) mast, an additional C-band imaging antenna

  16. Observing river stages using unmanned aerial vehicles

    NASA Astrophysics Data System (ADS)

    Niedzielski, Tomasz; Witek, Matylda; Spallek, Waldemar

    2016-08-01

    We elaborated a new method for observing water surface areas and river stages using unmanned aerial vehicles (UAVs). It is based on processing multitemporal five orthophotomaps produced from the UAV-taken visible light images of nine sites of the river, acquired with a sufficient overlap in each part. Water surface areas are calculated in the first place, and subsequently expressed as fractions of total areas of water-covered terrain at a given site of the river recorded on five dates. The logarithms of the fractions are later calculated, producing five samples, each consisted of nine elements. In order to detect statistically significant increments of water surface areas between two orthophotomaps, we apply the asymptotic and bootstrapped versions of the Student's t test, preceded by other tests that aim to check model assumptions. The procedure is applied to five orthophotomaps covering nine sites of the Ścinawka river (south-western (SW) Poland). The data have been acquired during the experimental campaign, at which flight settings were kept unchanged over nearly 3 years (2012-2014). We have found that it is possible to detect transitions between water surface areas associated with all characteristic water levels (low, mean, intermediate and high stages). In addition, we infer that the identified transitions hold for characteristic river stages as well. In the experiment we detected all increments of water level: (1) from low stages to mean, intermediate and high stages; (2) from mean stages to intermediate and high stages; and (3) from intermediate stages to high stages. Potential applications of the elaborated method include verification of hydrodynamic models and the associated predictions of high flows as well as monitoring water levels of rivers in ungauged basins.

  17. Mapping Forest Edge Using Aerial Lidar

    NASA Astrophysics Data System (ADS)

    MacLean, M. G.

    2014-12-01

    Slightly more than 60% of Massachusetts is covered with forest and this land cover type is invaluable for the protection and maintenance of our natural resources and is a carbon sink for the state. However, Massachusetts is currently experiencing a decline in forested lands, primarily due to the expansion of human development (Thompson et al., 2011). Of particular concern is the loss of "core areas" or the areas within forests that are not influenced by other land cover types. These areas are of significant importance to native flora and fauna, since they generally are not subject to invasion by exotic species and are more resilient to the effects of climate change (Campbell et al., 2009). However, the expansion of development has reduced the amount of this core area, but the exact amount is still unknown. Current methods of estimating core area are not particularly precise, since edge, or the area of the forest that is most influenced by other land cover types, is quite variable and situation dependent. Therefore, the purpose of this study is to devise a new method for identifying areas that could qualify as "edge" within the Harvard Forest, in Petersham MA, using new remote sensing techniques. We sampled along eight transects perpendicular to the edge of an abandoned golf course within the Harvard Forest property. Vegetation inventories as well as Photosynthetically Active Radiation (PAR) at different heights within the canopy were used to determine edge depth. These measurements were then compared with small-footprint waveform aerial LiDAR datasets and imagery to model edge depths within Harvard Forest.

  18. Exploring Planets with Directed Aerial Robot Explorers

    NASA Astrophysics Data System (ADS)

    Pankine, Alexey A.; Aaron, Kim M.; Heun, Matthew K.; Nock, Kerry T.; Schlaifer, R. Stephen; Ingersoll, Andrew P.; Lorenz, Ralph D.

    2004-02-01

    Global Aerospace Corporation (GAC) is developing a revolutionary system architecture for exploration of planetary atmospheres and surfaces from atmospheric altitudes. The work is supported by the NASA Institute for Advanced Concepts (NIAC). The innovative system architecture relies upon the use of Directed Aerial Robot Explorers (DAREs), which essentially are long-duration-flight autonomous balloons with trajectory control capabilities that can deploy swarms of miniature probes over multiple target areas. Balloon guidance capabilities will offer unprecedented opportunities in high-resolution, targeted observations of both atmospheric and surface phenomena. Multifunctional microprobes will be deployed from the balloons once over the target areas, and perform a multitude of functions, such as atmospheric profiling or surface exploration, relaying data back to the balloons or an orbiter. This architecture will enable low-cost, low-energy, long-term global exploration of planetary atmospheres and surfaces. This paper focuses on a conceptual analysis of the DARE architecture capabilities and science applications for Venus, Titan and Jupiter. Preliminary simulations with simplified atmospheric models show that a relatively small trajectory control wing can enable global coverage of the atmospheres of Venus and Titan by a single balloon over a 100-day mission. This presents unique opportunities for global in situ sampling of the atmospheric composition and dynamics, atmospheric profiling over multiple sites with small dropsondes and targeted deployment of surface microprobes. At Jupiter, path guidance capabilities of the DARE platforms permits targeting localized regions of interest, such as ``hot spots'' or the Great Red Spot. A single DARE platform at Jupiter can sample major types of the atmospheric flows (zones and belts) over a 100-day mission. Observations by deployable probes would reveal if the differences exist in radiative, dynamic and compositional environments

  19. 36 CFR 1237.24 - What are special considerations for storage and maintenance of aerial photographic records?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... maintenance of aerial photographic records? (a) Mark each aerial film container with a unique identification code to facilitate identification and filing. (b) Mark aerial film indexes with the unique aerial film identification codes or container codes for the aerial film that they index. Also, file and mark the...

  20. 36 CFR 1237.24 - What are special considerations for storage and maintenance of aerial photographic records?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... maintenance of aerial photographic records? (a) Mark each aerial film container with a unique identification code to facilitate identification and filing. (b) Mark aerial film indexes with the unique aerial film identification codes or container codes for the aerial film that they index. Also, file and mark the...

  1. 36 CFR 1237.24 - What are special considerations for storage and maintenance of aerial photographic records?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... maintenance of aerial photographic records? (a) Mark each aerial film container with a unique identification code to facilitate identification and filing. (b) Mark aerial film indexes with the unique aerial film identification codes or container codes for the aerial film that they index. Also, file and mark the...

  2. 36 CFR 1237.24 - What are special considerations for storage and maintenance of aerial photographic records?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... maintenance of aerial photographic records? (a) Mark each aerial film container with a unique identification code to facilitate identification and filing. (b) Mark aerial film indexes with the unique aerial film identification codes or container codes for the aerial film that they index. Also, file and mark the...

  3. Ceramide, cerebroside and triterpenoid saponin from the bark of aerial roots of Ficus elastica (Moraceae).

    PubMed

    Mbosso, Emmanuel Jean Teinkela; Nguedia, Jules Clément Assob; Meyer, Franck; Lenta, Bruno Ndjakou; Ngouela, Silvère; Lallemand, Benjamin; Mathieu, Véronique; Antwerpen, Pierre Van; Njunda, Anna Longdoh; Adiogo, Dieudonné; Tsamo, Etienne; Looze, Yvan; Kiss, Robert; Wintjens, René

    2012-11-01

    Three compounds, ficusamide (1), ficusoside (2) and elasticoside (3), were isolated from the bark of aerial roots of Ficus elastica (Moraceae), together with nine known compounds, including four triterpenes, three steroids and two aliphatic linear alcohols. The chemical structures of the three compounds were established by extensive 1D and 2D NMR spectroscopy, mass spectrometry and by comparison with published data. The growth inhibitory effect of the crude extract and isolated compounds was evaluated against several microorganisms and fungi. The cytotoxicity against human cancer cell lines was also assessed. Ficusamide (1) displayed a moderate in vitro growth inhibitory activity against the human A549 lung cancer cell line and a strong activity against Staphylococcus saprophyticus, while elasticoside (3) showed a potent activity on Enterococcus faecalis.

  4. Colorimetric Determination of Color of Aerial Mycelium of Streptomycetes1

    PubMed Central

    Lyons, Allister J.; Pridham, Thomas G.

    1965-01-01

    Lyons, Allister J., Jr. (Northern Regional Research Laboratory, Peoria, Ill.) and Thomas G. Pridham. Colorimetric determination of color of aerial mycelium of streptomycetes. J. Bacteriol. 89:159–169. 1965.—For some time, streptomycete taxonomists have been seeking to describe more accurately the colors of aerial mycelium. Some of the descriptive systems involve many different color names and groups. Others combine many colors into a few groups. All the systems and methods leave much to be desired. To obtain an accurate description, a colorimeter with a reflectance attachment was used to examine streptomycete aerial mycelium of 37 strains, representing all of the major aerial mycelium color groups. Each color was characterized by three values: dominant wavelength in millimicrons, and purity and brightness in percentages. All colors of aerial mycelium were of low purity (< 25%). Most of the dominant wavelengths were in the yellow to yellow-green bands of the spectrum. Most of the color tabs matched visually with the streptomycete strains had purities of a higher value than those of the cultures. The reflectance instrument seems to allow an objective description, and its use may help to clarify the color problem with streptomycetes. It is concluded that present color descriptions are inadequate and that the significance of color in speciation requires critical examination. PMID:14255657

  5. International-Aerial Measuring System (I-AMS) Training Program

    SciTech Connect

    Wasiolek, Piotre T.; Malchor, Russell L.; Maurer, Richard J.; Adams, Henry L.

    2015-10-01

    Since the Fukushima reactor accident in 2011, there has been an increased interest worldwide in developing national capabilities to rapidly map and assess ground contamination resulting from nuclear reactor accidents. The capability to rapidly measure the size of the contaminated area, determine the activity level, and identify the radionuclides can aid emergency managers and decision makers in providing timely protective action recommendations to the public and first responders. The development of an aerial detection capability requires interagency coordination to assemble the radiation experts, detection system operators, and aviation aircrews to conduct the aerial measurements, analyze and interpret the data, and provide technical assessments. The Office of International Emergency Management and Cooperation (IEMC) at the U.S. Department of Energy, National Nuclear Security Administration (DOE/NNSA) sponsors an International - Aerial Measuring System (I-AMS) training program for partner nations to develop and enhance their response to radiological emergencies. An initial series of courses can be conducted in the host country to assist in developing an aerial detection capability. As the capability develops and expands, additional experience can be gained through advanced courses with the opportunity to conduct aerial missions over a broad range of radiation environments.

  6. Arachnid aloft: directed aerial descent in neotropical canopy spiders.

    PubMed

    Yanoviak, Stephen P; Munk, Yonatan; Dudley, Robert

    2015-09-01

    The behaviour of directed aerial descent has been described for numerous taxa of wingless hexapods as they fall from the tropical rainforest canopy, but is not known in other terrestrial arthropods. Here, we describe similar controlled aerial behaviours for large arboreal spiders in the genus Selenops (Selenopidae). We dropped 59 such spiders from either canopy platforms or tree crowns in Panama and Peru; the majority (93%) directed their aerial trajectories towards and then landed upon nearby tree trunks. Following initial dorsoventral righting when necessary, falling spiders oriented themselves and then translated head-first towards targets; directional changes were correlated with bilaterally asymmetric motions of the anterolaterally extended forelegs. Aerial performance (i.e. the glide index) decreased with increasing body mass and wing loading, but not with projected surface area of the spider. Along with the occurrence of directed aerial descent in ants, jumping bristletails, and other wingless hexapods, this discovery of targeted gliding in selenopid spiders further indicates strong selective pressures against uncontrolled falls into the understory for arboreal taxa.

  7. Vehicle detection in aerial surveillance using dynamic Bayesian networks.

    PubMed

    Cheng, Hsu-Yung; Weng, Chih-Chia; Chen, Yi-Ying

    2012-04-01

    We present an automatic vehicle detection system for aerial surveillance in this paper. In this system, we escape from the stereotype and existing frameworks of vehicle detection in aerial surveillance, which are either region based or sliding window based. We design a pixelwise classification method for vehicle detection. The novelty lies in the fact that, in spite of performing pixelwise classification, relations among neighboring pixels in a region are preserved in the feature extraction process. We consider features including vehicle colors and local features. For vehicle color extraction, we utilize a color transform to separate vehicle colors and nonvehicle colors effectively. For edge detection, we apply moment preserving to adjust the thresholds of the Canny edge detector automatically, which increases the adaptability and the accuracy for detection in various aerial images. Afterward, a dynamic Bayesian network (DBN) is constructed for the classification purpose. We convert regional local features into quantitative observations that can be referenced when applying pixelwise classification via DBN. Experiments were conducted on a wide variety of aerial videos. The results demonstrate flexibility and good generalization abilities of the proposed method on a challenging data set with aerial surveillance images taken at different heights and under different camera angles.

  8. Arachnid aloft: directed aerial descent in neotropical canopy spiders.

    PubMed

    Yanoviak, Stephen P; Munk, Yonatan; Dudley, Robert

    2015-09-01

    The behaviour of directed aerial descent has been described for numerous taxa of wingless hexapods as they fall from the tropical rainforest canopy, but is not known in other terrestrial arthropods. Here, we describe similar controlled aerial behaviours for large arboreal spiders in the genus Selenops (Selenopidae). We dropped 59 such spiders from either canopy platforms or tree crowns in Panama and Peru; the majority (93%) directed their aerial trajectories towards and then landed upon nearby tree trunks. Following initial dorsoventral righting when necessary, falling spiders oriented themselves and then translated head-first towards targets; directional changes were correlated with bilaterally asymmetric motions of the anterolaterally extended forelegs. Aerial performance (i.e. the glide index) decreased with increasing body mass and wing loading, but not with projected surface area of the spider. Along with the occurrence of directed aerial descent in ants, jumping bristletails, and other wingless hexapods, this discovery of targeted gliding in selenopid spiders further indicates strong selective pressures against uncontrolled falls into the understory for arboreal taxa. PMID:26289654

  9. Arachnid aloft: directed aerial descent in neotropical canopy spiders

    PubMed Central

    Yanoviak, Stephen P.; Munk, Yonatan; Dudley, Robert

    2015-01-01

    The behaviour of directed aerial descent has been described for numerous taxa of wingless hexapods as they fall from the tropical rainforest canopy, but is not known in other terrestrial arthropods. Here, we describe similar controlled aerial behaviours for large arboreal spiders in the genus Selenops (Selenopidae). We dropped 59 such spiders from either canopy platforms or tree crowns in Panama and Peru; the majority (93%) directed their aerial trajectories towards and then landed upon nearby tree trunks. Following initial dorsoventral righting when necessary, falling spiders oriented themselves and then translated head-first towards targets; directional changes were correlated with bilaterally asymmetric motions of the anterolaterally extended forelegs. Aerial performance (i.e. the glide index) decreased with increasing body mass and wing loading, but not with projected surface area of the spider. Along with the occurrence of directed aerial descent in ants, jumping bristletails, and other wingless hexapods, this discovery of targeted gliding in selenopid spiders further indicates strong selective pressures against uncontrolled falls into the understory for arboreal taxa. PMID:26289654

  10. Aerial surveys and tagging of free-drifting icebergs using an unmanned aerial vehicle (UAV)

    NASA Astrophysics Data System (ADS)

    McGill, P. R.; Reisenbichler, K. R.; Etchemendy, S. A.; Dawe, T. C.; Hobson, B. W.

    2011-06-01

    Ship-based observations of free-drifting icebergs are hindered by the dangers of calving ice. To improve the efficacy and safety of these studies, new unmanned aerial vehicles (UAVs) were developed and then deployed in the Southern Ocean. These inexpensive UAVs were launched and recovered from a ship by scientific personal with a few weeks of flight training. The UAVs sent real-time video back to the ship, allowing researchers to observe conditions in regions of the icebergs not visible from the ship. In addition, the UAVs dropped newly developed global positioning system (GPS) tracking tags, permitting researchers to record the precise position of the icebergs over time. The position reports received from the tags show that the motion of free-drifting icebergs changes rapidly and is a complex combination of both translation and rotation.

  11. Robust adaptive control for Unmanned Aerial Vehicles

    NASA Astrophysics Data System (ADS)

    Kahveci, Nazli E.

    The objective of meeting higher endurance requirements remains a challenging task for any type and size of Unmanned Aerial Vehicles (UAVs). According to recent research studies significant energy savings can be realized through utilization of thermal currents. The navigation strategies followed across thermal regions, however, are based on rather intuitive assessments of remote pilots and lack any systematic path planning approaches. Various methods to enhance the autonomy of UAVs in soaring applications are investigated while seeking guarantees for flight performance improvements. The dynamics of the aircraft, small UAVs in particular, are affected by the environmental conditions, whereas unmodeled dynamics possibly become significant during aggressive flight maneuvers. Besides, the demanded control inputs might have a magnitude range beyond the limits dictated by the control surface actuators. The consequences of ignoring these issues can be catastrophic. Supporting this claim NASA Dryden Flight Research Center reports considerable performance degradation and even loss of stability in autonomous soaring flight tests with the subsequent risk of an aircraft crash. The existing control schemes are concluded to suffer from limited performance. Considering the aircraft dynamics and the thermal characteristics we define a vehicle-specific trajectory optimization problem to achieve increased cross-country speed and extended range of flight. In an environment with geographically dispersed set of thermals of possibly limited lifespan, we identify the similarities to the Vehicle Routing Problem (VRP) and provide both exact and approximate guidance algorithms for the navigation of automated UAVs. An additional stochastic approach is used to quantify the performance losses due to incorrect thermal data while dealing with random gust disturbances and onboard sensor measurement inaccuracies. One of the main contributions of this research is a novel adaptive control design with

  12. Historical integration of remote sensing data: Can GIS extract information from grayscale aerial photographs?

    NASA Astrophysics Data System (ADS)

    Robertson, Kristina Liane

    There have been many changes in land management policies of the National Forest system over the past 100 years. Changes in policy related to law, population growth and economics directly cause changes in land cover. Global land cover changes are occurring at such a pace and magnitude that they are affecting Earth system functioning (Lambin et al., 2001). The analysis of land cover changes plays a key role in understanding several environmental phenomena, resulting in a need for objective and comparable land cover maps (Gennaretti et al., 2011). Advances in remote sensing and Geographic Information Systems (GIS) have modernized land-use analysis but this technology has traditionally ignored historical black and white aerial photography (Kadmon & Harari-Kremer, 1998). The objectives of this thesis are to show that remote sensing and GIS can provide clear evidence of the consequences of major changes in land use polices using historical aerial images. The goal is to develop new techniques that will allow the use of these images and will widen the usefulness of GIS for environmental scientists interested in land cover change over the last 50-100 years. The research applies object base image analysis (OBIA) to four study sites that display physical evidence of different management strategies using the images collected over a 70 year time span. This project presents a semi-automatic object oriented method that will allow the analysis of landscape change by comparing historical aerial photographs with 2010 orthoimagery. The OBIA method provides many advantages over traditional classification methods by creating a standardized rule set that provides efficient segmentation, classification and creation of land cover maps for a large dataset of 29 diverse images.

  13. The Aeronautics Education, Research, and Industry Alliance (AERIAL) 2002 Report

    NASA Technical Reports Server (NTRS)

    Bowen, Brent D.; Box, Richard C.; Fink, Mary; Gogos, George; Lehrer, Henry R.; Narayanan, Ram M.; Nickerson, Jocelyn S.; Tarry, Scott E.; Vlasek, Karisa D.; O'Neil, Patrick D.

    2002-01-01

    The NASA Nebraska Space Grant Consortium (NSGC) & EPSCoR programs at the University of Nebraska at Omaha are involved in a variety of innovative research activities. Such research is supported through the Aeronautics Education, Research, and Industry Alliance (AERIAL) and collaborative seed funds. AERIAL is a comprehensive, multi-faceted, five year NASA EPSCoR initiative that contributes substantially to the strategic research and technology priorities of NASA while intensifying Nebraska s rapidly growing aeronautics research and development endeavors. AERIAL includes three major collaborative research teams (CRTs) whose nexus is a common focus in aeronautics research. Each CRT - Small Aircraft Transportation System (SATS), Airborne Remote Sensing for Agricultural Research and Commercialization Applications (ARS), and Numerical Simulation of the Combustion of Fuel Droplets: Finite Rate Kinetics and Flame Zone Grid Adaptation (CEFD) -has a distinct research agenda. This program provides the template for funding of new and innovative research that emphasizes aerospace technology.

  14. Overview of meteorological measurements for aerial spray modeling.

    PubMed

    Rafferty, J E; Biltoft, C A; Bowers, J F

    1996-06-01

    The routine meteorological observations made by the National Weather Service have a spatial resolution on the order of 1,000 km, whereas the resolution needed to conduct or model aerial spray applications is on the order of 1-10 km. Routinely available observations also do not include the detailed information on the turbulence and thermal structure of the boundary layer that is needed to predict the transport, dispersion, and deposition of aerial spray releases. This paper provides an overview of the information needed to develop the meteorological inputs for an aerial spray model such as the FSCBG and discusses the different types of instruments that are available to make the necessary measurements.

  15. Antioxidant activity of aerial parts of Tribulus alatus in rats.

    PubMed

    Kadry, H; Abou Basha, L; El Gindi, O; Temraz, A

    2010-01-01

    The antioxidant activity of alcoholic extract of Tribulus alatus was investigated by determination of blood glutathione, serum ascorbic acid and serum superoxide dismutase in rats. All groups treated with aerial parts without fruit, fruits and total herb showed a significant increase in all measured parameters (P<0.05). Upon fractionation of the alcoholic extracts using solvents with different polarities, all fractions revealed a significant increase in serum superoxide dismutase (P<0.05). On the other hand chloroformic fraction of aerial parts without fruit extract and ethylacetate fraction of fruits extract exhibited a significant increase in blood glutathione level. All fractions of fruits extract, chloroformic and ethylacetate fractions of aerial parts without fruit extract significantly increase the serum ascorbic acid concentration (P<0.05).

  16. PSM and thin OMOG reticles aerial imaging metrology comparison study

    NASA Astrophysics Data System (ADS)

    Cohen, Yaron; Finders, Jo; Mangan, Shmoolik; Englard, Ilan; Mouraille, Orion; Janssen, Maurice; Miyazaki, Junji; Connolly, Brid; Kojima, Yosuke; Higuchi, Masaru

    2012-02-01

    For sub 20nm features, IC (integrated circuits) designs include an increasing number of features approaching the resolution limits of the scanner compared to the previous generation of IC designs. This trend includes stringent design rules and complex, ever smaller optical proximity correction (OPC) structures. In this regime, a new type of mask, known as opaque MoSi on glass (OMOG), has been introduced to overcome the shortcomings of the well-established phase shift masks (PSM). This paper reviews the fundamental aerial imaging differences between identically designed PSM and thin OMOG masks. The masks were designed for scanner qualification tests and therefore contain large selections of 1D and 2D features, including various biases and OPCs. Aerial critical dimension uniformity (CDU) performance for various features on both masks are reported. Furthermore, special efforts have been made to emphasize the advantages of aerial imaging metrology versus wafer metrology in terms of shortening scanner qualification cycle time.

  17. Exploration of Titan Using Vertical Lift Aerial Vehicles

    NASA Technical Reports Server (NTRS)

    Young, L. A.

    2001-01-01

    Autonomous vertical lift aerial vehicles (such as rotorcraft or powered-lift vehicles) hold considerable potential for supporting planetary science and exploration missions. Vertical lift aerial vehicles would have the following advantages/attributes for planetary exploration: (1) low-speed and low-altitude detailed aerial surveys; (2) remote-site sample return to lander platforms; (3) precision placement of scientific probes; (4) soft landing capability for vehicle reuse (multiple flights) and remote-site monitoring; (5) greater range, speed, and access to hazardous terrain than a surface rover; and (6) greater resolution of surface details than an orbiter or balloons. Exploration of Titan presents an excellent opportunity for the development and usage of such vehicles. Additional information is contained in the original extended abstract.

  18. Radiological Disaster Simulators for Field and Aerial Measurements

    SciTech Connect

    H. W. Clark, Jr

    2002-11-01

    Simulators have been developed to dramatically improve the fidelity of play for field monitors and aircraft participating in radiological disaster drills and exercises. Simulated radiological measurements for the current Global Positioning System (GPS) location are derived from realistic models of radiological consequences for accidents and malicious acts. The aerial version outputs analog pulses corresponding to the signal that would be produced by various NaI (Tl) detectors at that location. The field monitor version reports the reading for any make/model of survey instrument selected. Position simulation modes are included in the aerial and field versions. The aerial version can generate a flight path based on input parameters or import an externally generated sequence of latitude and longitude coordinates. The field version utilizes a map-based point and click/drag interface to generate individual or a sequence of evenly spaced instrument measurements.

  19. Aerial Observation Needs Workshop, May 13-14, 2015

    SciTech Connect

    Nasiri, Shaima; Serbin, Shawn; Lesmes, David; Petty, Rick; Schmid, Beat; Vogelmann, Andrew; de Boer, Gijs; Dafflon, Baptiste; Guenther, Alex; Moore, David

    2015-10-01

    The mission of the Climate and Environmental Sciences Division (CESD) of the Office of Biological and Environmental Research (BER) within the U.S. Department of Energy's (DOE) Office of Science is "to advance a robust, predictive understanding of Earth's climate and environmental systems and to inform the development of sustainable solutions to the nation's energy and environmental challenges." Accomplishing this mission requires aerial observations of the atmospheric and terrestrial components of the climate system. CESD is assessing its current and future aerial observation needs to develop a strategy and roadmap of capability requirements for the next decade. To facilitate this process, a workshop was convened that consisted of invited experts in the atmospheric and terrestrial sciences, airborne observations, and modeling. This workshop report summarizes the community input prior to and during the workshop on research challenges and opportunities, as well as specific science questions and observational needs that require aerial observations to address.

  20. Growth inhibition of Candida by human oral epithelial cells.

    PubMed

    Steele, C; Leigh, J; Swoboda, R; Fidel, P L

    2000-11-01

    Oropharyngeal candidiasis (OPC) caused by Candida albicans is a significant problem in human immunodeficiency virus (HIV)-infected persons. Recognizing the paucity of information on innate and/or adaptive mucosal host defenses against C. albicans, we recently reported that human and nonhuman primate and mouse vaginal epithelial cells inhibit the growth of C. albicans in vitro. In the present study, oral epithelial cells collected from saliva of healthy volunteers and a purified oral epithelial cell line were found to inhibit blastoconidia and/or hyphal growth of several Candida species. Cell contact was a strict requirement for the epithelial cell anti-Candida activity; neither saliva nor culture supernatants alone inhibited Candida growth, and addition of saliva to the coculture did not modulate the epithelial cell activity. Finally, epithelial cell anti-Candida activity was significantly lower in HIV-infected persons with OPC. Together, these results suggest that oral epithelial cells may play a role in innate resistance against OPC.

  1. A pseudouridine residue in the spliceosome core is part of the filamentous growth program in yeast

    PubMed Central

    Basak, Anindita; Query, Charles C.

    2014-01-01

    SUMMARY Pseudouridine nucleobases, while abundant in tRNAs, rRNAs, and snRNAs, are not known to have physiologic roles in cell differentiation. We have identified a novel pseudouridine residue (Ψ28) on spliceosomal U6 snRNA that is induced during filamentous growth of Saccharomyces cerevisiae. Pus1p catalyzes this modification and is up-regulated during filamentation. Several U6 snRNA mutants are strongly pseudouridylated at Ψ28; remarkably, these U6 mutants activate pseudo-hyphal growth, dependent upon Pus1p, arguing that U6-Ψ28 per se can initiate at least part of the filamentous growth program, a conclusion confirmed using a designer snoRNA targeting U6-U28 pseudouridylation. Conversely, mutants that block U6-U28 pseudouridylation inhibit pseudo-hyphal growth. U6-U28 pseudouridylation changes the efficiency of splicing of suboptimal introns; thus, Pus1p-dependent pseudouridylation of U6 snRNA contributes to the filamentation growth program. PMID:25127136

  2. Wafer weak point detection based on aerial images or WLCD

    NASA Astrophysics Data System (ADS)

    Ning, Guoxiang; Philipp, Peter; Litt, Lloyd C.; Ackmann, Paul; Crell, Christian; Chen, Norman

    2015-10-01

    Aerial image measurement is a key technique for model based optical proximity correction (OPC) verification. Actual aerial images obtained by AIMS (aerial image measurement system) or WLCD (wafer level critical dimension) can detect printed wafer weak point structures in advance of wafer exposure and defect inspection. Normally, the potential wafer weak points are determined based on optical rule check (ORC) simulation in advance. However, the correlation to real wafer weak points is often not perfect due to the contribution of mask three dimension (M3D) effects, actual mask errors, and scanner lens effects. If the design weak points can accurately be detected in advance, it will reduce the wafer fab cost and improve cycle time. WLCD or AIMS tools are able to measure the aerial images CD and bossung curve through focus window. However, it is difficult to detect the wafer weak point in advance without defining selection criteria. In this study, wafer weak points sensitive to mask mean-to-nominal values are characterized for a process with very high MEEF (normally more than 4). Aerial image CD uses fixed threshold to detect the wafer weak points. By using WLCD through threshold and focus window, the efficiency of wafer weak point detection is also demonstrated. A novel method using contrast range evaluation is shown in the paper. Use of the slope of aerial images for more accurate detection of the wafer weak points using WLCD is also discussed. The contrast range can also be used to detect the wafer weak points in advance. Further, since the mean to nominal of the reticle contributes to the effective contrast range in a high MEEF area this work shows that control of the mask error is critical for high MEEF layers such as poly, active and metal layers. Wafer process based weak points that cannot be detected by wafer lithography CD or WLCD will be discussed.

  3. Aerospace toxicology overview: aerial application and cabin air quality.

    PubMed

    Chaturvedi, Arvind K

    2011-01-01

    Aerospace toxicology is a rather recent development and is closely related to aerospace medicine. Aerospace toxicology can be defined as a field of study designed to address the adverse effects of medications, chemicals, and contaminants on humans who fly within or outside the atmosphere in aviation or on space flights. The environment extending above and beyond the surface of the Earth is referred to as aerospace. The term aviation is frequently used interchangeably with aerospace. The focus of the literature review performed to prepare this paper was on aerospace toxicology-related subject matters, aerial application and aircraft cabin air quality. Among the important topics addressed are the following: · Aerial applications of agricultural chemicals, pesticidal toxicity, and exposures to aerially applied mixtures of chemicals and their associated formulating solvents/surfactants The safety of aerially encountered chemicals and the bioanalytical methods used to monitor exposures to some of them · The presence of fumes and smoke, as well as other contaminants that may generally be present in aircraft/space vehicle cabin air · And importantly, the toxic effects of aerially encountered contaminants, with emphasis on the degradation products of oils, fluids, and lubricants used in aircraft, and finally · Analytical methods used for monitoring human exposure to CO and HCN are addressed in the review, as are the signs and symptoms associated with exposures to these combustion gases. Although many agricultural chemical monitoring studies have been published, few have dealt with the occurrence of such chemicals in aircraft cabin air. However, agricultural chemicals do appear in cabin air; indeed, attempts have been made to establish maximum allowable concentrations for several of the more potentially toxic ones that are found in aircraft cabin air. In this article, I emphasize the need for precautionary measures to be taken to minimize exposures to aerially

  4. Spectral anomaly methods for aerial detection using KUT nuisance rejection

    NASA Astrophysics Data System (ADS)

    Detwiler, R. S.; Pfund, D. M.; Myjak, M. J.; Kulisek, J. A.; Seifert, C. E.

    2015-06-01

    This work discusses the application and optimization of a spectral anomaly method for the real-time detection of gamma radiation sources from an aerial helicopter platform. Aerial detection presents several key challenges over ground-based detection. For one, larger and more rapid background fluctuations are typical due to higher speeds, larger field of view, and geographically induced background changes. As well, the possible large altitude or stand-off distance variations cause significant steps in background count rate as well as spectral changes due to increased gamma-ray scatter with detection at higher altitudes. The work here details the adaptation and optimization of the PNNL-developed algorithm Nuisance-Rejecting Spectral Comparison Ratios for Anomaly Detection (NSCRAD), a spectral anomaly method previously developed for ground-based applications, for an aerial platform. The algorithm has been optimized for two multi-detector systems; a NaI(Tl)-detector-based system and a CsI detector array. The optimization here details the adaptation of the spectral windows for a particular set of target sources to aerial detection and the tailoring for the specific detectors. As well, the methodology and results for background rejection methods optimized for the aerial gamma-ray detection using Potassium, Uranium and Thorium (KUT) nuisance rejection are shown. Results indicate that use of a realistic KUT nuisance rejection may eliminate metric rises due to background magnitude and spectral steps encountered in aerial detection due to altitude changes and geographically induced steps such as at land-water interfaces.

  5. Aerospace toxicology overview: aerial application and cabin air quality.

    PubMed

    Chaturvedi, Arvind K

    2011-01-01

    Aerospace toxicology is a rather recent development and is closely related to aerospace medicine. Aerospace toxicology can be defined as a field of study designed to address the adverse effects of medications, chemicals, and contaminants on humans who fly within or outside the atmosphere in aviation or on space flights. The environment extending above and beyond the surface of the Earth is referred to as aerospace. The term aviation is frequently used interchangeably with aerospace. The focus of the literature review performed to prepare this paper was on aerospace toxicology-related subject matters, aerial application and aircraft cabin air quality. Among the important topics addressed are the following: · Aerial applications of agricultural chemicals, pesticidal toxicity, and exposures to aerially applied mixtures of chemicals and their associated formulating solvents/surfactants The safety of aerially encountered chemicals and the bioanalytical methods used to monitor exposures to some of them · The presence of fumes and smoke, as well as other contaminants that may generally be present in aircraft/space vehicle cabin air · And importantly, the toxic effects of aerially encountered contaminants, with emphasis on the degradation products of oils, fluids, and lubricants used in aircraft, and finally · Analytical methods used for monitoring human exposure to CO and HCN are addressed in the review, as are the signs and symptoms associated with exposures to these combustion gases. Although many agricultural chemical monitoring studies have been published, few have dealt with the occurrence of such chemicals in aircraft cabin air. However, agricultural chemicals do appear in cabin air; indeed, attempts have been made to establish maximum allowable concentrations for several of the more potentially toxic ones that are found in aircraft cabin air. In this article, I emphasize the need for precautionary measures to be taken to minimize exposures to aerially

  6. Preliminary assessment of aerial photography techniques for canvasback population analysis

    USGS Publications Warehouse

    Munro, R.E.; Trauger, D.L.

    1976-01-01

    Recent intensive research on the canvasback has focused attention on the need for more precise estimates of population parameters. During the 1972-75 period, various types of aerial photographing equipment were evaluated to determine the problems and potentials for employing these techniques in appraisals of canvasback populations. The equipment and procedures available for automated analysis of aerial photographic imagery were also investigated. Serious technical problems remain to be resolved, but some promising results were obtained. Final conclusions about the feasibility of operational implementation await a more rigorous analysis of the data collected.

  7. Review of the SAFARI 2000 RC-10 Aerial Photography

    NASA Technical Reports Server (NTRS)

    Myers, Jeff; Shelton, Gary; Annegarn, Harrold; Peterson, David L. (Technical Monitor)

    2001-01-01

    This presentation will review the aerial photography collected by the NASA ER-2 aircraft during the SAFARI (Southern African Regional Science Initiative) year 2000 campaign. It will include specifications on the camera and film, and will show examples of the imagery. It will also detail the extent of coverage, and the procedures to obtain film products from the South African government. Also included will be some sample applications of aerial photography for various environmental applications, and its use in augmenting other SAFARI data sets.

  8. A review of the meteorological parameters which affect aerial application

    NASA Technical Reports Server (NTRS)

    Christensen, L. S.; Frost, W.

    1979-01-01

    The ambient wind field and temperature gradient were found to be the most important parameters. Investigation results indicated that the majority of meteorological parameters affecting dispersion were interdependent and the exact mechanism by which these factors influence the particle dispersion was largely unknown. The types and approximately ranges of instrumented capabilities for a systematic study of the significant meteorological parameters influencing aerial applications were defined. Current mathematical dispersion models were also briefly reviewed. Unfortunately, a rigorous dispersion model which could be applied to aerial application was not available.

  9. Application of Adaptive Autopilot Designs for an Unmanned Aerial Vehicle

    NASA Technical Reports Server (NTRS)

    Shin, Yoonghyun; Calise, Anthony J.; Motter, Mark A.

    2005-01-01

    This paper summarizes the application of two adaptive approaches to autopilot design, and presents an evaluation and comparison of the two approaches in simulation for an unmanned aerial vehicle. One approach employs two-stage dynamic inversion and the other employs feedback dynamic inversions based on a command augmentation system. Both are augmented with neural network based adaptive elements. The approaches permit adaptation to both parametric uncertainty and unmodeled dynamics, and incorporate a method that permits adaptation during periods of control saturation. Simulation results for an FQM-117B radio controlled miniature aerial vehicle are presented to illustrate the performance of the neural network based adaptation.

  10. Supporting Remote Sensing Research with Small Unmanned Aerial Systems

    NASA Astrophysics Data System (ADS)

    Anderson, R. C.; Shanks, P. C.; Kritis, L. A.; Trani, M. G.

    2014-11-01

    We describe several remote sensing research projects supported with small Unmanned Aerial Systems (sUAS) operated by the NGA Basic and Applied Research Office. These sUAS collections provide data supporting Small Business Innovative Research (SBIR), NGA University Research Initiative (NURI), and Cooperative Research And Development Agreements (CRADA) efforts in addition to inhouse research. Some preliminary results related to 3D electro-optical point clouds are presented, and some research goals discussed. Additional details related to the autonomous operational mode of both our multi-rotor and fixed wing small Unmanned Aerial System (sUAS) platforms are presented.

  11. A study of methods for lowering aerial environmental survey cost

    NASA Technical Reports Server (NTRS)

    Stansberry, J. R.

    1973-01-01

    The results are presented of a study of methods for lowering the cost of environmental aerial surveys. A wide range of low cost techniques were investigated for possible application to current pressing urban and rural problems. The objective of the study is to establish a definition of the technical problems associated with conducting aerial surveys using various low cost techniques, to conduct a survey of equipment which may be used in low cost systems, and to establish preliminary estimates of cost. A set of candidate systems were selected and described for the environmental survey tasks.

  12. Aeronautic Instruments. Section VI : Aerial Navigation and Navigating Instruments

    NASA Technical Reports Server (NTRS)

    Eaton, H N

    1923-01-01

    This report outlines briefly the methods of aerial navigation which have been developed during the past few years, with a description of the different instruments used. Dead reckoning, the most universal method of aerial navigation, is first discussed. Then follows an outline of the principles of navigation by astronomical observation; a discussion of the practical use of natural horizons, such as sea, land, and cloud, in making extant observations; the use of artificial horizons, including the bubble, pendulum, and gyroscopic types. A description is given of the recent development of the radio direction finder and its application to navigation.

  13. Towards aerial natural gas leak detection system based on TDLAS

    NASA Astrophysics Data System (ADS)

    Liu, Shuyang; Zhou, Tao; Jia, Xiaodong

    2014-11-01

    Pipeline leakage is a complex scenario for sensing system due to the traditional high cost, low efficient and labor intensive detection scheme. TDLAS has been widely accepted as industrial trace gas detection method and, thanks to its high accuracy and reasonable size, it has the potential to meet pipeline gas leakage detection requirements if it combines with the aerial platform. Based on literature study, this paper discussed the possibility of applying aerial TDLAS principle in pipeline gas leak detection and the key technical foundation of implementing it. Such system is able to result in a high efficiency and accuracy measurement which will provide sufficient data in time for the pipeline leakage detection.

  14. The Role of Unmanned Aerial Systems-Sensors in Air Quality Research

    EPA Science Inventory

    The use of unmanned aerial systems (UASs) and miniaturized sensors for a variety of scientific and security purposes has rapidly increased. UASs include aerostats (tethered balloons) and remotely controlled, unmanned aerial vehicles (UAVs) including lighter-than-air vessels, fix...

  15. An automatic high precision registration method between large area aerial images and aerial light detection and ranging data

    NASA Astrophysics Data System (ADS)

    Du, Q.; Xie, D.; Sun, Y.

    2015-06-01

    The integration of digital aerial photogrammetry and Light Detetion And Ranging (LiDAR) is an inevitable trend in Surveying and Mapping field. We calculate the external orientation elements of images which identical with LiDAR coordinate to realize automatic high precision registration between aerial images and LiDAR data. There are two ways to calculate orientation elements. One is single image spatial resection using image matching 3D points that registered to LiDAR. The other one is Position and Orientation System (POS) data supported aerotriangulation. The high precision registration points are selected as Ground Control Points (GCPs) instead of measuring GCPs manually during aerotriangulation. The registration experiments indicate that the method which registering aerial images and LiDAR points has a great advantage in higher automation and precision compare with manual registration.

  16. Autonomous Aerial Sensors for Wind Power Meteorology

    NASA Astrophysics Data System (ADS)

    Giebel, Gregor; Schmidt Paulsen, Uwe; Reuder, Joachim; La Cour-Harbo, Anders; Thomsen, Carsten; Bange, Jens; Buschmann, Marco

    2010-05-01

    This poster describes a new approach for measurements in wind power meteorology using small unmanned flying platforms. During a week of flying a lighter-than-air vehicle, two small electrically powered aeroplanes and a larger helicopter at the Risø test station at Høvsøre, we will compare wind speed measurements with fixed mast and LIDAR measurements, investigate optimal flight patterns for each measurement task, and measure other interesting meteorological features like the air-sea boundary in the vicinity of the wind farm. In order to prepare the measurement campaign, a workshop is held, soliciting input from various communities. Large-scale wind farms, especially offshore, need an optimisation between installed wind power density and the losses in the wind farm due to wake effects between the turbines. While the wake structure behind single wind turbines onshore is fairly well understood, there are different problems offshore, thought to be due mainly to the low turbulence. Good measurements of the wake and wake structure are not easy to come by, as the use of a met mast is static and expensive, while the use of remote sensing instruments either needs significant access to the turbine to mount an instrument, or is complicated to use on a ship due to the ship's own movement. In any case, a good LIDAR or SODAR will cost many tens of thousands of euros. Another current problem in wind energy is the coming generation of wind turbines in the 10-12 MW class, with tip heights of over 200 m. Very few measurement masts exist to verify our knowledge of atmospheric physics - all that is known is that the boundary layer description we used so far is not valid any more. Here, automated Unmanned Aerial Vehicles (UAVs) could be used as either an extension of current high masts or to build a network of very high ‘masts' in a region of complex terrain or coastal flow conditions. In comparison to a multitude of high masts, UAVs could be quite cost-effective. In order to test

  17. Aerial robot navigation in cluttered urban environments

    NASA Astrophysics Data System (ADS)

    Shi, Dongqing

    Autonomous navigation systems for mobile robots have been successfully deployed for a wide range of planar ground-based tasks. However, very few counterparts of the previous planar navigation systems were developed for three-dimensional (3-D) motion, which is needed for unmanned aerial vehicles (UAVs). Safe maneuvering in complex environments is a major challenge for UAVs. Future urban reconnaissance and search missions will require UAVs to autonomously navigate through cluttered urban spaces. This research proposes two approaches for unmanned helicopter navigation in cluttered urban environments: a 3-D fuzzy behavioral approach and a 3-D vector field histogram (VFH) approach. Behavior-based control has been very successful for planar mobile robots navigation in unknown environments. A novel fuzzy behavioral scheme for navigating an unmanned helicopter in cluttered 3-D spaces is developed. The 3-D navigation problem is decomposed into several identical two-dimensional (2-D) navigation sub-problems, each of which is solved by using preference-based fuzzy behaviors. Due to the shortcomings of vector summation during the fusion of the 2-D sub-problems, instead of directly outputting steering subdirections by their own defuzzification processes, the undefuzzified intermediate results of the sub-problems are fused to a 3-D solution region, representing degrees of preference for the robot movement. A new defuzzification algorithm that steers the robot by finding the centroid of a 3-D convex region of maximum volume in the 3-D solution region is developed. A fuzzy speed control system is also developed to ensure the efficiency and safety of the navigation. The VFH approach is very popular for planar mobile robots. A 3-D VFH approach to UAV navigation in cluttered urban environments is developed. A 3-D laser measurement system is used to obtain the obstacle distribution in this method. Instead of a 2-D Cartesian histogram grid as a world model, a 3-D spherical histogram

  18. Aerial Flyover of New Research Facilities

    ScienceCinema

    None

    2016-07-12

    The Idaho National Laboratory is focused on continued development of its primary campus areas, including our Idaho Falls campus, to enable the INL to meet DOE expectations as the nations lead nuclear energy laboratory. This video identifies some of the existing Idaho Falls campus facilities and highlights planned and potential future development to support campus growth. You can learn more about INL's energy research projects at http://www.facebook.com/idahonationallaboratory.

  19. 77 FR 35962 - Utilizing Rapidly Deployable Aerial Communications Architecture in Response to an Emergency

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-15

    ... deployment of any special user devices include unmanned aerial vehicles, weather balloons, and suitcase based...- effectiveness of unmanned aerial vehicles, weather balloons, and high altitude platforms. How does the cost... COMMISSION Utilizing Rapidly Deployable Aerial Communications Architecture in Response to an Emergency...

  20. Unmanned aerial vehicles for rangeland mapping and monitoring: a comparison of two systems

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Aerial photography from unmanned aerial vehicles (UAVs) bridges the gap between ground-based observations and remotely sensed imagery from aerial and satellite platforms. UAVs can be deployed quickly and repeatedly, are less costly and safer than piloted aircraft, and can obtain very high-resolution...

  1. Off-the-Wall Project Brings Aerial Mapping down to Earth

    ERIC Educational Resources Information Center

    Davidhazy, Andrew

    2008-01-01

    The technology of aerial photography, photogrametry, has widespread applications in mapping and aerial surveying. A multi-billion-dollar industry, aerial surveying and mapping is "big business" in both civilian and military sectors. While the industry has grown increasingly automated, employment opportunities still exist for people with a basic…

  2. Current status and future directions of precision agriculture for aerial application in the USA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Precision aerial application in the USA is less than a decade old since the development of the first variable-rate aerial application system. Many areas of the United States rely on readily available agricultural airplanes or helicopters for pest management. Variable-rate aerial application provides...

  3. The Ground Control Room as an Enabling Technology in the Unmanned Aerial System

    NASA Technical Reports Server (NTRS)

    Gear, Gary; Mace, Thomas

    2007-01-01

    This viewgraph presentation reviews the development of the ground control room as an required technology for the use of an Unmanned Aerial system. The Unmanned Aerial system is a strategic component of the Global Observing System, which will serve global science needs. The unmanned aerial system will use the same airspace as manned aircraft, therefore there will be unique telemetry needs.

  4. Isolation and Identification of Potential Allelochemicals from Aerial Parts of Avena fatua L. and Their Allelopathic Effect on Wheat.

    PubMed

    Liu, Xingang; Tian, Fajun; Tian, Yingying; Wu, Yanbing; Dong, Fengshou; Xu, Jun; Zheng, Yongquan

    2016-05-11

    Five compounds (syringic acid, tricin, acacetin, syringoside, and diosmetin) were isolated from the aerial parts of wild oats (Avena fatua L.) using chromatography columns of silica gel and Sephadex LH-20. Their chemical structures were identified by means of electrospray ionization and high-resolution mass spectrometry as well as (1)H and (13)C nuclear magnetic resonance spectroscopic analyses. Bioassays showed that the five compounds had significant allelopathic effects on the germination and seedling growth of wheat (Triticum aestivum L.). The five compounds inhibited fresh wheat as well as the shoot and root growth of wheat by approximately 50% at a concentration of 100 mg/kg, except for tricin and syringoside for shoot growth. The results of activity testing indicated that the aerial parts of wild oats had strong allelopathic potential and could cause different degrees of influence on surrounding plants. Moreover, these compounds could be key allelochemicals in wild-oat-infested wheat fields and interfere with wheat growth via allelopathy.

  5. Isolation and Identification of Potential Allelochemicals from Aerial Parts of Avena fatua L. and Their Allelopathic Effect on Wheat.

    PubMed

    Liu, Xingang; Tian, Fajun; Tian, Yingying; Wu, Yanbing; Dong, Fengshou; Xu, Jun; Zheng, Yongquan

    2016-05-11

    Five compounds (syringic acid, tricin, acacetin, syringoside, and diosmetin) were isolated from the aerial parts of wild oats (Avena fatua L.) using chromatography columns of silica gel and Sephadex LH-20. Their chemical structures were identified by means of electrospray ionization and high-resolution mass spectrometry as well as (1)H and (13)C nuclear magnetic resonance spectroscopic analyses. Bioassays showed that the five compounds had significant allelopathic effects on the germination and seedling growth of wheat (Triticum aestivum L.). The five compounds inhibited fresh wheat as well as the shoot and root growth of wheat by approximately 50% at a concentration of 100 mg/kg, except for tricin and syringoside for shoot growth. The results of activity testing indicated that the aerial parts of wild oats had strong allelopathic potential and could cause different degrees of influence on surrounding plants. Moreover, these compounds could be key allelochemicals in wild-oat-infested wheat fields and interfere with wheat growth via allelopathy. PMID:27079356

  6. Structural design and fabrication techniques of composite unmanned aerial vehicles

    NASA Astrophysics Data System (ADS)

    Hunt, Daniel Stephen

    Popularity of unmanned aerial vehicles has grown substantially in recent years both in the private sector, as well as for government functions. This growth can be attributed largely to the increased performance of the technology that controls these vehicles, as well as decreasing cost and size of this technology. What is sometimes forgotten though, is that the research and advancement of the airframes themselves are equally as important as what is done with them. With current computer-aided design programs, the limits of design optimization can be pushed further than ever before, resulting in lighter and faster airframes that can achieve longer endurances, higher altitudes, and more complex missions. However, realization of a paper design is still limited by the physical restrictions of the real world and the structural constraints associated with it. The purpose of this paper is to not only step through current design and manufacturing processes of composite UAVs at Oklahoma State University, but to also focus on composite spars, utilizing and relating both calculated and empirical data. Most of the experience gained for this thesis was from the Cessna Longitude project. The Longitude is a 1/8 scale, flying demonstrator Oklahoma State University constructed for Cessna. For the project, Cessna required dynamic flight data for their design process in order to make their 2017 release date. Oklahoma State University was privileged enough to assist Cessna with the mission of supporting the validation of design of their largest business jet to date. This paper will detail the steps of the fabrication process used in construction of the Longitude, as well as several other projects, beginning with structural design, machining, molding, skin layup, and ending with final assembly. Also, attention will be paid specifically towards spar design and testing in effort to ease the design phase. This document is intended to act not only as a further development of current

  7. 60. Aerial view looking southeast; Dundee Dam and Passaic River ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    60. Aerial view looking southeast; Dundee Dam and Passaic River at center, Dundee Canal and headgates, guardlock, and former hydroelectric facility at right, Dundee Textile Mill between river and canal - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  8. 59. Aerial view looking west; Dundee Textile Mill at front ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    59. Aerial view looking west; Dundee Textile Mill at front center, Dundee Canal at center - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  9. Dead Slow: Unmanned Aerial Vehicles Loitering in Battlespace

    ERIC Educational Resources Information Center

    Blackmore, Tim

    2005-01-01

    Unmanned (or Uninhabited) Aerial Vehicles are a key part of the American military's so-called revolution in military affairs (RMA) as practiced over Iraq. They are also part of the drive to shift agency away from humans and toward machines. This article considers the ways in which humans have, in calling on high technologies to distance them from…

  10. Unmanned Aerial Vehicle Use for Wood Chips Pile Volume Estimation

    NASA Astrophysics Data System (ADS)

    Mokroš, M.; Tabačák, M.; Lieskovský, M.; Fabrika, M.

    2016-06-01

    The rapid development of unmanned aerial vehicles is a challenge for applied research. Many technologies are developed and then researcher are looking up for their application in different sectors. Therefore, we decided to verify the use of the unmanned aerial vehicle for wood chips pile monitoring. We compared the use of GNSS device and unmanned aerial vehicle for volume estimation of four wood chips piles. We used DJI Phantom 3 Professional with the built-in camera and GNSS device (geoexplorer 6000). We used Agisoft photoscan for processing photos and ArcGIS for processing points. Volumes calculated from pictures were not statistically significantly different from amounts calculated from GNSS data and high correlation between them was found (p = 0.9993). We conclude that the use of unmanned aerial vehicle instead of the GNSS device does not lead to significantly different results. Tthe data collection consumed from almost 12 to 20 times less time with the use of UAV. Additionally, UAV provides documentation trough orthomosaic.

  11. Aerial view of the entire bridge crossing the Tennessee River ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Aerial view of the entire bridge crossing the Tennessee River looking up river. The swing bridge, when open, permits river navigational traffic to ply the river. Construction of a replacement bridge, to be located 93.27 feet down river, has now started. - Bridgeport Swing Span Bridge, Spanning Tennessee River, Bridgeport, Jackson County, AL

  12. 9. AERIAL VIEW LOOKING NORTH AT THE GEORGE C. MARSHALL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. AERIAL VIEW LOOKING NORTH AT THE GEORGE C. MARSHALL SPACE FLIGHT CENTER. DODD ROAD RUNS DOWN THE CENTER OF THE PHOTO. THE EAST TEST AREA IS TOWARDS THE BOTTOM OF THE PHOTO, FABRICATION, ENGINEERING AND ADMINISTRATION NEAR THE TOP OF THE PHOTO. 1961, MSFC PHOTO LAB. - Marshall Space Flight Center, East Test Area, Dodd Road, Huntsville, Madison County, AL

  13. A HIGHER ALTITUDE AERIAL PHOTOGRAPH TAKEN IN 1970, LOOKING SOUTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    A HIGHER ALTITUDE AERIAL PHOTOGRAPH TAKEN IN 1970, LOOKING SOUTH TOWARD THE HUACHUCA MOUNTAINS. THE 1916 STABLES ARE LOCATED IN THE RIGHT MIDDLE PORTION OF THE PICTURE (FORT HUACHUCA HISTORICAL MUSEUM, PHOTOGRAPH 1970.15.00.59, PHOTOGRAPHER UNIDENTIFIED, CREATED BY AND PROPERTY OF THE UNITED STATES ARMY) - Fort Huachuca, Cavalry Stables, Clarkson Road, Sierra Vista, Cochise County, AZ

  14. Maps--Map Reading and Aerial Photography. [2 Units].

    ERIC Educational Resources Information Center

    Haakonsen, Harry O., Ed.

    Included in this set of materials are two units: (1) Maps and Map Reading and (2) Aerial Photography. Each unit includes student guide sheets, reference material, and tape script. A set of 35mm slides and audiotapes are usually used with the materials. The unit on Maps and Map Reading is designed to develop map reading skills and the use of these…

  15. A scheduling model for the aerial relay system

    NASA Technical Reports Server (NTRS)

    Ausrotas, R. A.; Liu, E. W.

    1980-01-01

    The ability of the Aerial Relay System to handle the U.S. transcontinental large hub passenger flow was analyzed with a flexible, interactive computer model. The model incorporated city pair time of day demand and a demand allocation function which assigned passengers to their preferred flights.

  16. Aerial view, view north with Walkers Mill left of the ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Aerial view, view north with Walkers Mill left of the creek, Henry Clay village right of creek, Tyler-Mcconnell Bridge in middleground, and Hagley area beyond the bridge - Charles I. Du Pont House, 162 Main Street, Wilmington, New Castle County, DE

  17. AERIAL VIEW, LAUREL HILL CEMETERY (LEFT) AND MOUNT PEACE CEMETERY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AERIAL VIEW, LAUREL HILL CEMETERY (LEFT) AND MOUNT PEACE CEMETERY (RIGHT). LOCATED ACROSS RIDGE AVENUE FROM LAUREL HILL CEMETERY, MOUNT PEACE CEMETERY WAS FOUNDED BY THE ODD FELLOWS CEMETERY COMPANY IN 1865. - Laurel Hill Cemetery, 3822 Ridge Avenue, Philadelphia, Philadelphia County, PA

  18. 6. AERIAL VIEW OF THE PATH TRANSIT SYSTEM BRIDGE LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. AERIAL VIEW OF THE PATH TRANSIT SYSTEM BRIDGE LOOKING SOUTHEAST. TO THE RIGHT ARE THE NEWARK TURNPIKE AND THE CONRAIL BRIDGE (HAER No. NJ-43). THE PULASKI SKYWAY (HAER No. NJ-34) IS IN THE BACKGROUND - Path Transit System Bridge, Spanning Hackensack River, Kearny, Hudson County, NJ

  19. 2. AERIAL VIEW OF THE VERTICAL LIFT BRIDGES SPANNING THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. AERIAL VIEW OF THE VERTICAL LIFT BRIDGES SPANNING THE HACKENSACK RIVER, LOOKING NORTHEAST. THE PATH TRANSIT BRIDGE IS IN THE FOREGROUND, WITH THE CONRAIL (HAER No. NJ-43), NEWARK TURNPIKE, AND ERIE & LACKAWANNA RAILROAD (HAER No. NJ-42) BRIDGES BEHIND IT - Path Transit System Bridge, Spanning Hackensack River, Kearny, Hudson County, NJ

  20. 5. AERIAL VIEW OF THE PATH TRANSIT SYSTEM BRIDGE LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. AERIAL VIEW OF THE PATH TRANSIT SYSTEM BRIDGE LOOKING SOUTHEAST. TO THE RIGHT ARE THE NEWARK TURNPIKE AND THE CONRAIL BRIDGE (HAER No. NJ-43). THE PULASKI SKYWAY (HAER No. NJ-34) IS IN THE BACKGROUND - Path Transit System Bridge, Spanning Hackensack River, Kearny, Hudson County, NJ