Science.gov

Sample records for afa catcher vessels

  1. 50 CFR Table 41 to Part 679 - BSAI Crab PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 11 2011-10-01 2011-10-01 false BSAI Crab PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels 41 Table 41 to Part 679 Wildlife and Fisheries FISHERY... Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels For the following crab species in...

  2. 50 CFR Table 41 to Part 679 - BSAI Crab PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 13 2012-10-01 2012-10-01 false BSAI Crab PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels 41 Table 41 to Part 679 Wildlife and Fisheries FISHERY... Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels For the following crab species in...

  3. 50 CFR Table 41 to Part 679 - BSAI Crab PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 13 2014-10-01 2014-10-01 false BSAI Crab PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels 41 Table 41 to Part 679 Wildlife and Fisheries FISHERY... Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels For the following crab species in...

  4. 50 CFR Table 40 to Part 679 - BSAI Halibut PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 9 2010-10-01 2010-10-01 false BSAI Halibut PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels 40 Table 40 to Part 679 Wildlife and Fisheries FISHERY... Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels In the following target...

  5. 50 CFR Table 40 to Part 679 - BSAI Halibut PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 13 2014-10-01 2014-10-01 false BSAI Halibut PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels 40 Table 40 to Part 679 Wildlife and Fisheries FISHERY... Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels In the following target...

  6. 50 CFR Table 41 to Part 679 - BSAI Crab PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 13 2013-10-01 2013-10-01 false BSAI Crab PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels 41 Table 41 to Part 679 Wildlife and Fisheries FISHERY... Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels For the following crab species in...

  7. 50 CFR Table 41 to Part 679 - BSAI Crab PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 9 2010-10-01 2010-10-01 false BSAI Crab PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels 41 Table 41 to Part 679 Wildlife and Fisheries FISHERY... Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels For the following crab species in...

  8. 50 CFR Table 40 to Part 679 - BSAI Halibut PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 11 2011-10-01 2011-10-01 false BSAI Halibut PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels 40 Table 40 to Part 679 Wildlife and Fisheries FISHERY... Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels In the following target...

  9. 50 CFR Table 40 to Part 679 - BSAI Halibut PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 13 2012-10-01 2012-10-01 false BSAI Halibut PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels 40 Table 40 to Part 679 Wildlife and Fisheries FISHERY... Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels In the following target...

  10. 50 CFR Table 40 to Part 679 - BSAI Halibut PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 13 2013-10-01 2013-10-01 false BSAI Halibut PSC Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels 40 Table 40 to Part 679 Wildlife and Fisheries FISHERY... Sideboard Limits for AFA Catcher/Processors and AFA Catcher Vessels In the following target...

  11. 50 CFR 679.63 - Catch weighing requirements for vessels and processors.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... requirements for listed AFA catcher/processors and AFA motherships?—(1) Catch weighing. All groundfish landed by listed AFA catcher/processors or received by AFA motherships must be weighed on a NMFS-certified... AFA catcher/processor or an AFA mothership must ensure that the vessel is in compliance with the...

  12. 50 CFR 679.63 - Catch weighing requirements for vessels and processors.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... requirements for listed AFA catcher/processors and AFA motherships?—(1) Catch weighing. All groundfish landed by listed AFA catcher/processors or received by AFA motherships must be weighed on a NMFS-certified... AFA catcher/processor or an AFA mothership must ensure that the vessel is in compliance with the...

  13. 50 CFR 679.63 - Catch weighing requirements for vessels and processors.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... requirements for listed AFA catcher/processors and AFA motherships?—(1) Catch weighing. All groundfish landed by listed AFA catcher/processors or received by AFA motherships must be weighed on a NMFS-certified... AFA catcher/processor or an AFA mothership must ensure that the vessel is in compliance with the...

  14. 50 CFR 679.63 - Catch weighing requirements for vessels and processors.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... requirements for listed AFA catcher/processors and AFA motherships?—(1) Catch weighing. All groundfish landed by listed AFA catcher/processors or received by AFA motherships must be weighed on a NMFS-certified... AFA catcher/processor or an AFA mothership must ensure that the vessel is in compliance with the...

  15. 76 FR 79764 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel DREAM CATCHER; Invitation for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF TRANSPORTATION Maritime Administration Requested Administrative Waiver of the Coastwise Trade Laws: Vessel DREAM CATCHER... the vessel DREAM CATCHER is: INTENDED COMMERCIAL USE OF VESSEL: ``Passenger charter.''...

  16. New sacrificial material for ex-vessel core catcher

    NASA Astrophysics Data System (ADS)

    Komlev, Andrei A.; Almjashev, Vyacheslav I.; Bechta, Sevostian V.; Khabensky, Vladimir B.; Granovsky, Vladimir S.; Gusarov, Victor V.

    2015-12-01

    A new functional (sacrificial) material has been developed in the Fe2O3-SrO-Al2O3-CaO system based on strontium hexaferrite ceramic in concrete matrix. The method of producing SM has been advanced technologically; this technological effectiveness allows the SM to be used in ex-vessel core catchers with corium spreading as well as in crucible-type core catchers. Critical properties regarding the efficiency of SM in ex-vessel core catchers, such as porosity, pycnometric density, apparent density, solidus and liquidus temperatures, and water content have been measured. Suitable fractions of SrFe12O19 and high alumina cement (HAC) were found in the SM based on thermodynamic analysis of the SM/corium interaction. The use of sacrificial steel as an additional heat adsorption component in the core catcher allowed us to increase the mass fraction range of SrFe12O19 in the SM from 0.3-0.5 to 0.3-0.85. The activation temperature of the SM/corium interaction has been shown to correspond to the liquidus temperature of the local composition at the SM/corium interface. The calculated value of this temperature was 1716 °C. Analysis of phase transformations in the SrO-Fe2O3 system revealed advantages of the SrFe12O19-based sacrificial material compared with the Fe2O3-contained material owing to the time proximity of SrFe12O19 decomposition and corium interaction activation.

  17. 75 FR 44927 - Fisheries of the Exclusive Economic Zone Off Alaska; Pollock for American Fisheries Act Catcher...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-30

    ...: NMFS is prohibiting directed fishing for pollock by American Fisheries Act (AFA) trawl catcher vessels... CFR part 679. The 2010 Bering Sea pollock TAC allocated to the AFA inshore open access fishery in the....gov/sustainablefisheries/afa/10bsaicoopallocations.pdf . In accordance with Sec. 679.20(d)(1)(i),...

  18. 78 FR 18896 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Less Than 60...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-28

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Less Than 60 feet (18.3 meters) Length Overall... directed fishing for Pacific cod by catcher vessels less than 60 feet (18.3 meters (m)) length overall...

  19. 76 FR 12606 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Less Than 60...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-08

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Less Than 60 Feet (18.3 m) Length Overall Using... for Pacific cod by catcher vessels less than 60 feet (18.3 m) length overall (LOA) using jig or...

  20. 78 FR 15643 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-12

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl Gear in the Bering Sea and Aleutian... for Pacific cod by catcher vessels using trawl gear in the Bering Sea and Aleutian Islands management... Pacific cod total allowable catch allocated to trawl catcher vessels in the BSAI. DATES: Effective...

  1. 77 FR 19147 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-30

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl Gear in the Bering Sea and Aleutian... directed fishing for Pacific cod by catcher vessels using trawl gear in the Bering Sea and Aleutian Islands... cod total allowable catch (TAC) allocated to trawl catcher vessels in the BSAI. DATES: Effective...

  2. 77 FR 23159 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-18

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl Gear in the Bering Sea and Aleutian... for Pacific cod by catcher vessels using trawl gear in the Bering Sea and Aleutian Islands management... directed fishing for Pacific cod by catcher vessels using trawl gear in the BSAI. After the effective...

  3. 76 FR 20891 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-14

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl Gear in the Bering Sea and Aleutian... directed fishing for Pacific cod by catcher vessels using trawl gear in the Bering Sea and Aleutian Islands... allowable catch (TAC) of Pacific cod allocated to catcher vessels using trawl gear in the BSAI....

  4. 75 FR 16359 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-01

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl Gear in the Bering Sea and Aleutian... for Pacific cod by catcher vessels using trawl gear in the Bering Sea and Aleutian Islands management... cod allowable catch (TAC) specified for catcher vessels using trawl gear in the BSAI. DATES:...

  5. 76 FR 22057 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-20

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl Gear in the Bering Sea and Aleutian... directed fishing for Pacific cod by catcher vessels using trawl gear in the Bering Sea and Aleutian Islands... allowable catch (TAC) of Pacific cod allocated to catcher vessels using trawl gear in the BSAI....

  6. 75 FR 12463 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-16

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl Gear in the Bering Sea and Aleutian... for Pacific cod by catcher vessels using trawl gear in the Bering Sea and Aleutian Islands management... cod allowable catch (TAC) specified for catcher vessels using trawl gear in the BSAI. DATES:...

  7. 76 FR 18663 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-05

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl Gear in the Bering Sea and Aleutian... for Pacific cod by catcher vessels using trawl gear in the Bering Sea and Aleutian Islands management... cod allowable catch (TAC) specified for catcher vessels using trawl gear in the BSAI. DATES:...

  8. 75 FR 41999 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Ocean Perch for Catcher Vessels...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-20

    ... Economic Zone Off Alaska; Pacific Ocean Perch for Catcher Vessels Participating in the Rockfish Entry Level... vessels participating in the rockfish entry level fishery in the Central Regulatory Area of the Gulf of... perch for trawl catcher vessels participating in the rockfish entry level fishery in the...

  9. 76 FR 40838 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Ocean Perch for Catcher Vessels...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-12

    ... Economic Zone Off Alaska; Pacific Ocean Perch for Catcher Vessels Participating in the Rockfish Entry Level... vessels participating in the rockfish entry level fishery in the Central Regulatory Area of the Gulf of... Pacific ocean perch for trawl catcher vessels participating in the rockfish entry level fishery in...

  10. 76 FR 46207 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Ocean Perch for Catcher Vessels...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-02

    ... Economic Zone Off Alaska; Pacific Ocean Perch for Catcher Vessels Participating in the Rockfish Entry Level... vessels participating in the rockfish entry level fishery in the Central Regulatory Area of the Gulf of... Pacific ocean perch for trawl catcher vessels participating in the rockfish entry level fishery in...

  11. 77 FR 11412 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-27

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl Gear in the Western Regulatory Area of... for Pacific cod by catcher vessels (CVs) using trawl gear in the Western Regulatory Area of the Gulf... Pacific cod total allowable catch apportioned to CVs using trawl gear in the Western Regulatory Area...

  12. 78 FR 18528 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-27

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl Gear in the Central Regulatory Area of... for Pacific cod by catcher vessels (CVs) using trawl gear in the Central Regulatory Area of the Gulf... Pacific cod total allowable catch apportioned to CVs using trawl gear in the Central Regulatory Area...

  13. 77 FR 20571 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Hook...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-05

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Hook-and-Line Gear in the Western Regulatory... directed fishing for Pacific cod by catcher vessels (CVs) using hook-and-line gear in the Western... allowance of the 2012 Pacific cod total allowable catch apportioned to CVs using hook-and-line gear in...

  14. 78 FR 25004 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Hook...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-29

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Hook-and-Line Gear in the Western Regulatory... directed fishing for Pacific cod by catcher vessels (CVs) using hook-and-line gear in the Western... allowance of the 2013 Pacific cod total allowable catch apportioned to CVs using hook-and-line gear in...

  15. 78 FR 11790 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-20

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl Gear in the Western Regulatory Area of... for Pacific cod by catcher vessels (CVs) using trawl gear in the Western Regulatory Area of the Gulf... Pacific cod total allowable catch apportioned to CVs using trawl gear in the Western Regulatory Area...

  16. 75 FR 8841 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Less Than 60...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-26

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Less Than 60 feet (18.3 m) Length Overall Using... Islands Management Area AGENCY: National Marine Fisheries Service (NMFS), National Oceanic and Atmospheric... for Pacific cod by catcher vessels less than 60 feet (18.3 m) length overall (LOA) using jig or...

  17. 77 FR 19564 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-02

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl Gear in the Central Regulatory Area of... for Pacific cod by catcher vessels (CVs) using trawl gear in the Central Regulatory Area of the Gulf... Pacific cod total allowable catch apportioned to CVs using trawl gear in the Central Regulatory Area...

  18. 75 FR 38936 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Ocean Perch for Catcher Vessels...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-07

    ... Economic Zone Off Alaska; Pacific Ocean Perch for Catcher Vessels Participating in the Rockfish Entry Level... participating in the rockfish entry level fishery in the Central Regulatory Area of the Gulf of Alaska (GOA... perch for trawl catcher vessels participating in the rockfish entry level fishery in the...

  19. 75 FR 5251 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Greater Than...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-02

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Greater Than or Equal to 60 Feet (18.3 Meters... vessels greater than or equal to 60 feet (18.3 meters (m)) length overall (LOA) in the Bering Sea...

  20. 78 FR 5144 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Greater Than...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-24

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Greater Than or Equal To 60 Feet (18.3 Meters... vessels greater than or equal to 60 feet (18.3 meters (m)) length overall (LOA) in the Bering Sea...

  1. 76 FR 4081 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Greater Than...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-24

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Greater Than or Equal to 60 Feet (18.3 Meters... vessels greater than or equal to 60 feet (18.3 meters (m)) length overall (LOA) in the Bering Sea...

  2. 77 FR 3157 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Greater Than...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-23

    ... Zone Off Alaska; Pacific Cod by Catcher Vessels Greater Than or Equal To 60 Feet (18.3 Meters) Length... vessels greater than or equal to 60 feet (18.3 meters (m)) length overall (LOA) in the Bering Sea...

  3. Design and Evaluation of an Enhanced In-Vessel Core Catcher

    SciTech Connect

    Joy L. Rempe

    2004-06-01

    An enhanced in-vessel core catcher is being designed and evaluated as part of a joint United States (U.S.) - Korean International Nuclear Engineering Research Initiative (INERI) investigating methods to insure In-Vessel Retention (IVR) of core materials that may relocate under severe accident conditions in advanced reactors. To reduce cost and simplify manufacture and installation, this new core catcher design consists of several interlocking sections that are machined to fit together when inserted into the lower head. If needed, the core catcher can be manufactured with holes to accommodate lower head penetrations. Each section of the core catcher consists of two material layers with an option to add a third layer (if deemed necessary): a base material, which has the capability to support and contain the mass of core materials that may relocate during a severe accident; an oxide coating material on top of the base material, which resists interactions with high-temperature core materials; and an optional coating on the bottom side of the base material to prevent any potential oxidation of the base material during the lifetime of the reactor. This paper summarizes the status of core catcher design and evaluation efforts, including analyses, materials interaction tests, and prototypic testing efforts.

  4. 78 FR 17885 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Less Than 50...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-25

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Less Than 50 Feet (15.2 Meters) Length Overall Using Hook-and-Line Gear in the Central Regulatory Area of the Gulf of Alaska AGENCY: National Marine...: Temporary rule; closure. SUMMARY: NMFS is prohibiting directed fishing for Pacific cod by catcher...

  5. 78 FR 17884 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Greater Than...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-25

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Greater Than or Equal to 50 Feet (15.2 Meters...: National Marine Fisheries Service (NMFS), National Oceanic and Atmospheric Administration (NOAA), Commerce... vessels (CVs) greater than or equal to 50 feet (15.2 meters (m)) in length overall (LOA) using...

  6. 77 FR 13510 - Pacific Cod by Catcher Vessels Less Than 50 Feet (15.2 Meters) Length Overall Using Hook-and-Line...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-07

    ... Vessels Less Than 50 Feet (15.2 Meters) Length Overall Using Hook-and-Line Gear in the Central Regulatory... directed fishing for Pacific cod by catcher vessels (CVs) less than 50 feet (15.2 meters (m)) in...

  7. 77 FR 10400 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Less Than 60...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-22

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Less Than 60 Feet (18.3 Meters) Length Overall... less than 60 feet (18.3 meters (m)) length overall (LOA) using hook-and-line or pot gear in the...

  8. 78 FR 9328 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Less Than 60...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-08

    ... Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Less Than 60 Feet (18.3 Meters) Length Overall... less than 60 feet (18.3 meters (m)) length overall (LOA) using hook-and-line or pot gear in the...

  9. 75 FR 15626 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Less Than 60...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-30

    ... Hook-and-Line or Pot Gear in the Bering Sea and Aleutian Islands Management Area AGENCY: National... Aleutian Islands management area (BSAI). This action is necessary to prevent exceeding the 2010 Pacific cod total allowable catch allocated to catcher vessels less than 60 feet LOA using hook-and-line or pot...

  10. 75 FR 72735 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Less Than 60...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-26

    ... Hook-and-Line or Pot Gear in the Bering Sea and Aleutian Islands Management Area AGENCY: National... and Aleutian Islands management area (BSAI). This action is necessary to prevent exceeding the 2010 Pacific cod total allowable catch allocated to catcher vessels less than 60 feet LOA using...

  11. 75 FR 28502 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Less Than 60...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-21

    ... Hook-and-Line or Pot Gear in the Bering Sea and Aleutian Islands Management Area AGENCY: National... Aleutian Islands management area (BSAI). This action is necessary to prevent exceeding the 2010 Pacific cod total allowable catch allocated to catcher vessels less than 60 feet LOA using hook-and-line or pot...

  12. 75 FR 70614 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Greater Than...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-18

    ...) Length Overall Using Pot Gear in the Bering Sea and Aleutian Islands Management Area AGENCY: National... Aleutian Islands management area (BSAI). This action is necessary to prevent exceeding the 2010 Pacific cod total allowable catch (TAC) specified for pot catcher vessels greater than or equal to 60 feet (18.3...

  13. 76 FR 13098 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Less Than 60...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-10

    ... Hook-and-Line or Pot Gear in the Bering Sea and Aleutian Islands Management Area AGENCY: National... Aleutian Islands management area (BSAI). This action is necessary to prevent exceeding the 2011 Pacific cod total allowable catch allocated to catcher vessels less than 60 feet LOA using hook-and-line or pot...

  14. 50 CFR 679.63 - Catch weighing requirements for vessels and processors.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... published at 79 FR 54602, Sept. 12, 2014. (a) What are the requirements for listed AFA catcher/processors and AFA motherships?—(1) Catch weighing. All groundfish landed by listed AFA catcher/processors or received by AFA motherships must be weighed on a NMFS-certified scale and made available for sampling by...

  15. 75 FR 54792 - Fisheries of the Exclusive Economic Zone Off Alaska; Reallocation of Pollock in the Bering Sea...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-09

    ... season AFA catcher vessels harvesting pollock for processing by AFA inshore processors, 170,384 mt to B season AFA catcher/processors and AFA catcher vessels delivering pollock to catcher/processors, and 42,596 mt to B season AFA catcher vessels harvesting pollock for processing by AFA motherships. This...

  16. A scaling study of the natural circulation flow of the ex-vessel core catcher cooling system of EU-APR1400 for designing a scale-down test facility for design verification

    SciTech Connect

    Rhee, B. W.; Ha, K. S.; Park, R. J.; Song, J. H.; Revankar, S. T.

    2012-07-01

    In this paper a scaling study on the steady state natural circulation flow along the flow path of the ex vessel core catcher cooling system of EU-APR1400 is described, and the scaling criteria for reproducing the same steady state thermalhydraulic characteristics of the natural circulation flow as a prototype core catcher cooling system in the scale-down test facility are derived in terms of the down-comer pipe diameter and orifice resistance. (authors)

  17. A study on the effect of various design parameters on the natural circulation flow rate of the ex-vessel core catcher cooling system of EU-APR1400

    SciTech Connect

    Rhee, B. W.; Ha, K. S.; Park, R. J.; Song, J. H.

    2012-07-01

    In this paper, a study on the effect of various design parameters such as the channel gap width, heat flux distribution, down-comer pipe size and two-phase flow slip ratio on the natural circulation flow rate is performed based on a physical model for a natural circulation flow along the flow path of the ex-vessel core catcher cooling system of an EU-APR1400, and these effects on the natural circulation flow rate are analyzed and compared with the minimum flow rate required for the safe operation of the system. (authors)

  18. The CARIBU gas catcher

    SciTech Connect

    Savard, G.; Levand, A. F.; Zabransky, B. J.

    2016-06-01

    The CARIBU upgrade of the ATLAS facility provides radioactive beams of neutron-rich isotopes for experiments at low and Coulomb barrier energies. It creates these beam using a large RF gas catcher that collects and cools fission fragments from an intense Cf-252 fission source and transforms them into a low-emittance monoenergetic beam. This beam can then be purified, reaccelerated and delivered to experiments. This technique is fast and universal, providing access to all fission fragment species independently of their chemical properties. The CARIBU gas catcher has been built to operate at high ionization density and in the presence of the contamination from the source. A brief overview of the CARIBU concept is given below, together with a more detailed description of the CARIBU gas catcher and the performance it has now achieved.

  19. The CARIBU gas catcher

    NASA Astrophysics Data System (ADS)

    Savard, G.; Levand, A. F.; Zabransky, B. J.

    2016-06-01

    The CARIBU upgrade of the ATLAS facility provides radioactive beams of neutron-rich isotopes for experiments at low and Coulomb barrier energies. It creates these beam using a large RF gas catcher that collects and cools fission fragments from an intense 252 Cf fission source and transforms them into a low-emittance monoenergetic beam. This beam can then be purified, reaccelerated and delivered to experiments. This technique is fast and universal, providing access to all fission fragment species independently of their chemical properties. The CARIBU gas catcher has been built to operate at high ionization density and in the presence of the contamination from the source. A brief overview of the CARIBU concept is given below, together with a more detailed description of the CARIBU gas catcher and the performance it has now achieved.

  20. 75 FR 38937 - Fisheries of the Economic Exclusive Zone Off Alaska; Deep-Water Species Fishery by Catcher...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-07

    ... Exclusive Zone Off Alaska; Deep-Water Species Fishery by Catcher Vessels in the Gulf of Alaska AGENCY... the deep-water species fishery for catcher vessels subject to sideboard limits established under the... Pacific halibut prohibited species catch (PSC) sideboard limit specified for the deep-water...

  1. 76 FR 39790 - Fisheries of the Economic Exclusive Zone Off Alaska; Deep-Water Species Fishery by Catcher...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-07

    ... Exclusive Zone Off Alaska; Deep-Water Species Fishery by Catcher Vessels in the Gulf of Alaska AGENCY... the deep-water species fishery for catcher vessels subject to sideboard limits established under the... Pacific halibut prohibited species catch (PSC) sideboard limit specified for the deep-water...

  2. 77 FR 16949 - Pacific Cod by Catcher Vessels Greater Than or Equal to 50 Feet (15.2 Meters) Length Overall...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-23

    ... Vessels Greater Than or Equal to 50 Feet (15.2 Meters) Length Overall Using Hook-and-Line Gear in the... meters (m)) in length overall (LOA) using hook-and-line gear in the Central Regulatory Area of the...

  3. Paint-Overspray Catcher

    NASA Technical Reports Server (NTRS)

    Weinstein, Leonard M.

    2003-01-01

    An apparatus to catch paint overspray has been proposed. Overspray is an unavoidable parasitic component of spray that occurs because the flow of air or other gas in the spray must turn at the sprayed surface. Very small droplets are carried away in this turning flow, and some land on adjacent surfaces not meant to be painted. The basic principle of the paint-spray catcher is to divert the overspray into a suction system at the boundary of the area to be painted. The paint-spray catcher (see figure) would include a toroidal plenum connected through narrow throat to a nozzle that would face toward the center of the torus, which would be positioned over the center of the area to be spray-painted. The plenum would be supported by four tubes that would also serve as suction exhaust ducts. The downstream ends of the tubes (not shown in the figure) would be connected to a filter on a suction pump. The pump would be rated to provide a suction mass flow somewhat greater than that of the directed spray gas stream, so that the nozzle would take in a small excess of surrounding gas and catch nearly all of the overspray. A small raised lip at the bottom edge of the nozzle would catch paint that landed inside the nozzle. Even if the paint is directly piston pumped, the droplets entrain an air flow by time they approach the wall, so there is always a gas stream to carry the excess droplets to the side. For long-duration spraying operations, it could be desirable to include a suction-drain apparatus to prevent overflowing and dripping of paint from inside the lip. A version without an external contraction and with the throat angled downward would be a more compact version of catcher, although it might be slightly less efficient.

  4. Dra/AfaE adhesin of uropathogenic Dr/Afa+ Escherichia coli mediates mortality in pregnant rats.

    PubMed

    Wroblewska-Seniuk, K; Selvarangan, R; Hart, A; Pladzyk, R; Goluszko, P; Jafari, A; du Merle, L; Nowicki, S; Yallampalli, C; Le Bouguénec, C; Nowicki, B

    2005-11-01

    Escherichia coli bearing adhesins of the Dr/Afa family frequently causes urogenital infections during pregnancy in humans and has been associated with mortality in pregnant rats. Two components of the adhesin, Dra/AfaE and Dra/AfaD, considered virulence factors, are responsible for bacterial binding and internalization. We hypothesize that gestational mortality caused by Dr/Afa+ E. coli is mediated by one of these two proteins, Dra/AfaE or Dra/AfaD. In this study, using afaE and/or afaD mutants, we investigated the role of the afaE and afaD genes in the mortality of pregnant rats from intrauterine infection. Sprague-Dawley rats, on the 17th day of pregnancy, were infected with the E. coli afaE+ afaD and afaE afaD+ mutants. The clinical E. coli strain (afaE+ afaD+) and the afaE afaD double mutant were used as positive and negative controls, respectively. The mortality rate was evaluated 24 h after infection. The highest maternal mortality was observed in the group infected with the afaE+ afaD+ strain, followed by the group infected with the afaE+ afaD strain. The mortality was dose dependent. The afaE afaD double mutant did not cause maternal mortality, even with the highest infection dose. The in vivo studies corresponded with the invasion assay, where the afaE+ strains were the most invasive (afaE+ afaD strain > afaE+ afaD+ strain), while the afaE mutant strains (afaE afaD+ and afaE afaD strains) seemed to be noninvasive. This study shows for the first time that the afaE gene coding for the AfaE subunit of Dr/Afa adhesin is involved in the lethal outcome of gestational infection in rats. This lethal effect associated with AfaE correlates with the invasiveness of afaE+ E. coli strains in vitro.

  5. Dra/AfaE Adhesin of Uropathogenic Dr/Afa+ Escherichia coli Mediates Mortality in Pregnant Rats

    PubMed Central

    Wroblewska-Seniuk, K.; Selvarangan, R.; Hart, A.; Pladzyk, R.; Goluszko, P.; Jafari, A.; du Merle, L.; Nowicki, S.; Yallampalli, C.; Le Bouguénec, C.; Nowicki, B.

    2005-01-01

    Escherichia coli bearing adhesins of the Dr/Afa family frequently causes urogenital infections during pregnancy in humans and has been associated with mortality in pregnant rats. Two components of the adhesin, Dra/AfaE and Dra/AfaD, considered virulence factors, are responsible for bacterial binding and internalization. We hypothesize that gestational mortality caused by Dr/Afa+ E. coli is mediated by one of these two proteins, Dra/AfaE or Dra/AfaD. In this study, using afaE and/or afaD mutants, we investigated the role of the afaE and afaD genes in the mortality of pregnant rats from intrauterine infection. Sprague-Dawley rats, on the 17th day of pregnancy, were infected with the E. coli afaE+ afaD and afaE afaD+ mutants. The clinical E. coli strain (afaE+ afaD+) and the afaE afaD double mutant were used as positive and negative controls, respectively. The mortality rate was evaluated 24 h after infection. The highest maternal mortality was observed in the group infected with the afaE+ afaD+ strain, followed by the group infected with the afaE+ afaD strain. The mortality was dose dependent. The afaE afaD double mutant did not cause maternal mortality, even with the highest infection dose. The in vivo studies corresponded with the invasion assay, where the afaE+ strains were the most invasive (afaE+ afaD strain > afaE+ afaD+ strain), while the afaE mutant strains (afaE afaD+ and afaE afaD strains) seemed to be noninvasive. This study shows for the first time that the afaE gene coding for the AfaE subunit of Dr/Afa adhesin is involved in the lethal outcome of gestational infection in rats. This lethal effect associated with AfaE correlates with the invasiveness of afaE+ E. coli strains in vitro. PMID:16239563

  6. 50 CFR 679.62 - Inshore sector cooperative allocation program.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... vessel cooperative that applies for and receives an AFA inshore cooperative fishing permit under § 679.4... an official AFA inshore cooperative catch history for every inshore-sector endorsed AFA catcher... the vessel's official AFA inshore cooperative catch history for each subarea. A vessel's best 2...

  7. 77 FR 72791 - Fisheries of the Exclusive Economic Zone Off Alaska; Bering Sea and Aleutian Islands; 2013 and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-06

    ... Under the American Fisheries Act (AFA) Section 679.20(a)(5)(i)(A) requires that the pollock TAC... harvest by AFA catcher vessels with catcher/ processor sector endorsements, unless the Regional Administrator receives a cooperative contract that provides for the distribution of harvest among AFA...

  8. 76 FR 12884 - Groundfish Fisheries of the Exclusive Economic Zone Off Alaska; American Fisheries Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-09

    ... pollock fishery by identifying the vessels and processors eligible to participate in the fishery and...'') among the AFA inshore sector (50 percent), the AFA catcher/processor sector (40 percent), and the AFA... cooperatives, two offshore catcher/ processor cooperatives, and one mothership cooperative. After...

  9. AFA Support for Research: Journals and Conferences.

    ERIC Educational Resources Information Center

    Sillars, Malcolm O.; Zarefsky, David

    2000-01-01

    Claims the American Forensic Association's (AFA) publications and conferences represent its principal commitment to research. Presents a review of the journals and conferences under AFA sponsorship. Reviews the strong commitment to research on the part of the AFA, particularly as it is manifested in the association's journal and in conferences.…

  10. Numerical and Experimental Model Studies on Thermal Hydraulic Behavior of FBR Internal Core Catcher Assembly

    SciTech Connect

    Sanjay Kumar Das; Anil Kumar Sharma; Jasmin Sudha, A.; Punitha, G.; Lydia, G.; Somayajulu, P.A.; Murthy, S.S.; Malarvizhi, B.; Gopalakrishnan, V.; Harvey, J.; Kasinathan, N.; Rajan, M.

    2006-07-01

    Core Catcher is provided as an in-vessel core debris retention device to collect, support, cool and maintain in sub-critical configuration, the generated core debris from fuel melting due to certain postulated Beyond Design Basis Events (BDBE) for Fast Breeder Reactor (FBR). This also acts as a barrier to prevent settling of debris on main vessel and keeps its maximum temperature within acceptable creep range. Heat transfer by natural convection in the core catcher assembly has been assessed numerically and through water experiments using geometrically similar configuration. Resistive heating elements are used in experiment as heat source to simulate debris decay heat on core catcher. Series of experiments were carried out for two configurations referred as geometry A and geometry B. The later configuration showed enhanced natural convective heat transfer from the lower plenum of the vessel. Temperatures were monitored at critical positions and compared with numerical evaluation. Numerically evaluated flow fields and isotherms are compared with experimental data for specific steady state temperatures on heat source plate. Numerical results are found to be in good agreement with that obtained from experiments. The combined efforts of numerical and experimental work conclude core catcher assembly with geometry B to be more suitable. (authors)

  11. 25 CFR 1000.180 - What is a successor AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false What is a successor AFA? 1000.180 Section 1000.180... § 1000.180 What is a successor AFA? A successor AFA is a funding agreement negotiated after a Tribe's... the AFA should generally use the terms of the existing AFA to expedite and simplify the exchange...

  12. 25 CFR 1000.180 - What is a successor AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false What is a successor AFA? 1000.180 Section 1000.180... § 1000.180 What is a successor AFA? A successor AFA is a funding agreement negotiated after a Tribe's... the AFA should generally use the terms of the existing AFA to expedite and simplify the exchange...

  13. 25 CFR 1000.180 - What is a successor AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false What is a successor AFA? 1000.180 Section 1000.180... § 1000.180 What is a successor AFA? A successor AFA is a funding agreement negotiated after a Tribe's... the AFA should generally use the terms of the existing AFA to expedite and simplify the exchange...

  14. 25 CFR 1000.180 - What is a successor AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false What is a successor AFA? 1000.180 Section 1000.180... § 1000.180 What is a successor AFA? A successor AFA is a funding agreement negotiated after a Tribe's... the AFA should generally use the terms of the existing AFA to expedite and simplify the exchange...

  15. 25 CFR 1000.180 - What is a successor AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false What is a successor AFA? 1000.180 Section 1000.180... § 1000.180 What is a successor AFA? A successor AFA is a funding agreement negotiated after a Tribe's... the AFA should generally use the terms of the existing AFA to expedite and simplify the exchange...

  16. On a cryogenic noble gas ion catcher

    NASA Astrophysics Data System (ADS)

    Dendooven, P.; Purushothaman, S.; Gloos, K.

    2006-03-01

    In situ purification of the gas used as stopping medium in a noble gas ion catcher by operating the device at low temperatures of 60-150 K was investigated. Alpha-decay recoil ions from a 223Ra source served as energetic probes. The combined ion survival and transport efficiencies for 219Rn ions saturated below about 90 K, reaching 28.7(17)% in helium, 22.1(13)% in neon, and 17.0(10)% in argon. These values may well reflect the charge exchange and stripping cross-sections during the slowing down of the ions, and thus represent a fundamental upper limit for the efficiency of noble gas ion catcher devices. We suggest the cryogenic noble gas ion catcher as a technically simpler alternative to the ultra-high purity noble gas ion catcher operating at room temperature.

  17. The AfaR small RNA controls expression of the AfaD-VIII invasin in pathogenic Escherichia coli strains

    PubMed Central

    Pichon, Christophe; du Merle, Laurence; Lequeutre, Isabelle; Le Bouguénec, Chantal

    2013-01-01

    Pathogenic Escherichia coli strains carrying the afa-8 gene cluster are frequently associated with extra-intestinal infections in humans and animals. The afa-8 A to E genes determine the formation of an afimbrial adhesive sheath consisting of the AfaD-VIII invasin and the AfaE-VIII adhesin at the bacterial cell surface. This structure is thought to be required for host colonization. We characterized a new gene encoding the small RNA AfaR, which is transcribed in cis from the complementary strand of the 3′ untranslated region of the afaD messenger RNA, within the afaD–afaE intercistronic region. AfaR is a trans-acting Hfq-dependent antisense small RNA that binds the 5′ untranslated region of the afaD messenger RNA, initiating several ribonuclease E-dependent cleavages, thereby downregulating production of the AfaD-VIII invasin. AfaR transcription is dependent on σE, a member of the stress response family of extracytoplasmic alternative sigma factors. We found that the AfaR-dependent regulatory pathway was controlled by temperature, allowing the production of the AfaD-VIII invasin at temperatures above 37°C. Our findings suggest that the entry of afa-8-positive pathogenic E. coli strains into epithelial cells is tightly regulated by the AfaR small RNA. PMID:23563153

  18. The afa-related gene cluster in necrotoxigenic and other Escherichia coli from animals belongs to the afa-8 variant.

    PubMed

    Gérardin, J; Lalioui, L; Jacquemin, E; Le Bouguénec, C; Mainil, J G

    2000-09-25

    Six hundred and nine necrotoxigenic Escherichia coli type 1 and 2 (NTEC1 and NTEC2) and non-NTEC isolated in Western and Southern Europe, North Africa and Canada from diseased calves, pigs, humans, poultry, and 55 isolated from asymptomatic calves were studied for the identification of afa-related sequences to the recently described afa-7 and afa-8 gene cluster variants from two bovine Escherichia coli (Lalioui et al., 1999). Colony hybridization and PCR assays for the afaD-7, afaE-7, afaD-8 and afaE-8 identified the afa-related sequences to the afa-8 gene cluster in most (67/79; 85%) of the E. coli positive with the Afa-f family probe and in 14 additional strains negative with the Afa-f probe. No E. coli was positive for the afa-7 gene cluster. The existence of afa-8 positive strains was thus confirmed among bovine E. coli and for the first time among porcine, poultry and human E. coli. Sequencing of the afaE-8 amplicon of nine strains from the different host species showed a high degree of conservation (>95% at the DNA level; >92% at the amino-acid level). The afa-8 gene cluster was more frequent in E. coli from diseased calves (18%) than from piglets (12%), humans (6%) and poultry (5%). Bovine NTEC2 (26%) were more frequently positive than NTEC 1 (20%) and non-NTEC (11%). E. coli isolated from asymptomatic calves were rarely positive: one NTEC2 (3%) and no non-NTEC. The afa-8 gene cluster was located on the Vir plasmid in 11/23 NTEC2, but no plasmid localization was detected in NTEC1 or non-NTEC.

  19. Adhesion to and invasion of HeLa cells by pathogenic Escherichia coli carrying the afa-3 gene cluster are mediated by the AfaE and AfaD proteins, respectively.

    PubMed

    Jouve, M; Garcia, M I; Courcoux, P; Labigne, A; Gounon, P; Le Bouguénec, C

    1997-10-01

    The afa-3 gene cluster, expressed by uropathogenic and diarrhea-associated Escherichia coli strains, determines the formation of an afimbrial adhesive sheath composed of the AfaD and AfaE-III adhesins. The adherence to HeLa cells by recombinant HB101 strains producing both or only one of these two adhesins was investigated. Ultrastructural analyses of the interaction and gentamicin protection assays showed adherence to HeLa cells by HB101 producing both the AfaD and AfaE-III proteins and internalization of a subpopulation of the bacteria into the cells. The interactions of HeLa cells either with HB101 mutants producing AfaD or AfaE-III or with polystyrene beads coated with purified His6-tagged AfaD or His6-tagged AfaE-III proteins were studied. These experiments demonstrated that AfaE-III allows binding to HeLa cells and that AfaD mediates the internalization of the adherent bacteria. Ultrastructural analyses of the interaction of His6-AfaD-gold complexes with HeLa cells confirmed that AfaD is able to bind to the HeLa cell surface and indicated that it penetrates the cells via clathrin vesicles. These data demonstrate that the afa gene cluster is unique among bacteria, as alone it encodes both adhesion to and invasion of epithelial cells.

  20. Molecular cloning and characterization of the afa-7 and afa-8 gene clusters encoding afimbrial adhesins in Escherichia coli strains associated with diarrhea or septicemia in calves.

    PubMed

    Lalioui, L; Jouve, M; Gounon, P; Le Bouguenec, C

    1999-10-01

    The afa gene clusters, which encode proteins involved in adhesion to epithelial cells, from Escherichia coli strains associated with urinary and intestinal infections in humans have been characterized. Pathogenic isolates of bovine and porcine origin that possess afa-related sequences have recently been described. We report in this work the cloning and characterization of the afa-7 and afa-8 gene clusters from bovine isolates. Hybridization and sequencing experiments revealed that despite similarity in genetic organization, the afa-7 and afa-8 genes, and the well-characterized afa-3 operon expressed by human-pathogenic isolates, correspond to three different members of the afa family of gene clusters. However, like the afa-3 gene cluster, both the afa-7 and afa-8 gene clusters were found to encode an afimbrial adhesin (AfaE) and an invasin (AfaD). The AfaD peptides encoded by the three gene clusters were only 45% identical, but functional complementation experiments indicated that they belong to the same family of invasins. Hemagglutination and adhesion assays demonstrated that the AfaE-VII and AfaE-VIII adhesins bind to different receptors and that these receptors are not the human decay-accelerating factor recognized to be the receptor of all previously described AfaE adhesins. The AfaE-VIII adhesin is very similar to the M agglutinin of human-uropathogenic strains. We used PCR assays to screen 25 bovine strains for afaD and afaE genes of either the afa-7 or afa-8 gene cluster. The afa-8 gene cluster was highly prevalent in bovine isolates previously reported to carry afa-related sequences (23 of 24 strains), particularly in strains producing cytotoxic necrotizing factors (16 of 16 strains). The location of the afa-8 gene cluster on the plasmids or chromosome of these isolates suggests that it could be carried by a mobile element, facilitating its dissemination among bovine-pathogenic E. coli strains.

  1. Catcher’s Mitt Final Report

    DTIC Science & Technology

    2011-08-30

    The Catcher’s Mitt study was conducted to evaluate the need for, and the technical feasibility of, reducing the amount of orbital debris via active...removal. The Defense Advanced Research Projects Agency (DARPA) with support from the Orbital Debris Office at NASA reached out to the aerospace...for a new DARPA program. Although there are many policy issues which need to be addressed related to orbital debris removal, the Cather’s Mitt study

  2. Combination pipe rupture mitigator and in-vessel core catcher

    DOEpatents

    Tilbrook, Roger W.; Markowski, Franz J.

    1983-01-01

    A device which mitigates against the effects of a failed coolant loop in a nuclear reactor by restricting the outflow of coolant from the reactor through the failed loop and by retaining any particulated debris from a molten core which may result from coolant loss or other cause. The device reduces the reverse pressure drop through the failed loop by limiting the access of coolant in the reactor to the inlet of the failed loop. The device also spreads any particulated core debris over a large area to promote cooling.

  3. 25 CFR 1000.148 - What happens if an AFA expires before the effective date of the successor AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false What happens if an AFA expires before the effective date of the successor AFA? 1000.148 Section 1000.148 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... Funding Agreements Reallocation, Duration, and Amendments § 1000.148 What happens if an AFA expires...

  4. 25 CFR 1000.148 - What happens if an AFA expires before the effective date of the successor AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false What happens if an AFA expires before the effective date of the successor AFA? 1000.148 Section 1000.148 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... Funding Agreements Reallocation, Duration, and Amendments § 1000.148 What happens if an AFA expires...

  5. 25 CFR 1000.148 - What happens if an AFA expires before the effective date of the successor AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false What happens if an AFA expires before the effective date of the successor AFA? 1000.148 Section 1000.148 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... Funding Agreements Reallocation, Duration, and Amendments § 1000.148 What happens if an AFA expires...

  6. 25 CFR 1000.90 - What happens if an AFA expires before the effective date of the successor AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false What happens if an AFA expires before the effective date of the successor AFA? 1000.90 Section 1000.90 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... Affairs Programs Determining What Programs May Be Included in An Afa § 1000.90 What happens if an...

  7. 25 CFR 1000.148 - What happens if an AFA expires before the effective date of the successor AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false What happens if an AFA expires before the effective date of the successor AFA? 1000.148 Section 1000.148 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... Funding Agreements Reallocation, Duration, and Amendments § 1000.148 What happens if an AFA expires...

  8. 25 CFR 1000.90 - What happens if an AFA expires before the effective date of the successor AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false What happens if an AFA expires before the effective date of the successor AFA? 1000.90 Section 1000.90 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... Affairs Programs Determining What Programs May Be Included in An Afa § 1000.90 What happens if an...

  9. 25 CFR 1000.90 - What happens if an AFA expires before the effective date of the successor AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false What happens if an AFA expires before the effective date of the successor AFA? 1000.90 Section 1000.90 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... Affairs Programs Determining What Programs May Be Included in An Afa § 1000.90 What happens if an...

  10. 25 CFR 1000.90 - What happens if an AFA expires before the effective date of the successor AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false What happens if an AFA expires before the effective date of the successor AFA? 1000.90 Section 1000.90 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... Affairs Programs Determining What Programs May Be Included in An Afa § 1000.90 What happens if an...

  11. 25 CFR 1000.90 - What happens if an AFA expires before the effective date of the successor AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false What happens if an AFA expires before the effective date of the successor AFA? 1000.90 Section 1000.90 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... Affairs Programs Determining What Programs May Be Included in An Afa § 1000.90 What happens if an...

  12. 25 CFR 1000.148 - What happens if an AFA expires before the effective date of the successor AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false What happens if an AFA expires before the effective date of the successor AFA? 1000.148 Section 1000.148 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... Funding Agreements Reallocation, Duration, and Amendments § 1000.148 What happens if an AFA expires...

  13. 25 CFR 1000.428 - How may a Tribe/Consortium appeal a decision made after the AFA or compact or amendment to an AFA...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... the AFA or compact or amendment to an AFA or compact has been signed? 1000.428 Section 1000.428... Appeals § 1000.428 How may a Tribe/Consortium appeal a decision made after the AFA or compact or amendment to an AFA or compact has been signed? With the exception of certain decisions concerning...

  14. 25 CFR 1000.428 - How may a Tribe/Consortium appeal a decision made after the AFA or compact or amendment to an AFA...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... the AFA or compact or amendment to an AFA or compact has been signed? 1000.428 Section 1000.428... Appeals § 1000.428 How may a Tribe/Consortium appeal a decision made after the AFA or compact or amendment to an AFA or compact has been signed? With the exception of certain decisions concerning...

  15. 25 CFR 1000.428 - How may a Tribe/Consortium appeal a decision made after the AFA or compact or amendment to an AFA...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... the AFA or compact or amendment to an AFA or compact has been signed? 1000.428 Section 1000.428... Appeals § 1000.428 How may a Tribe/Consortium appeal a decision made after the AFA or compact or amendment to an AFA or compact has been signed? With the exception of certain decisions concerning...

  16. 25 CFR 1000.428 - How may a Tribe/Consortium appeal a decision made after the AFA or compact or amendment to an AFA...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... the AFA or compact or amendment to an AFA or compact has been signed? 1000.428 Section 1000.428... Appeals § 1000.428 How may a Tribe/Consortium appeal a decision made after the AFA or compact or amendment to an AFA or compact has been signed? With the exception of certain decisions concerning...

  17. 25 CFR 1000.428 - How may a Tribe/Consortium appeal a decision made after the AFA or compact or amendment to an AFA...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... the AFA or compact or amendment to an AFA or compact has been signed? 1000.428 Section 1000.428... Appeals § 1000.428 How may a Tribe/Consortium appeal a decision made after the AFA or compact or amendment to an AFA or compact has been signed? With the exception of certain decisions concerning...

  18. 50 CFR 679.51 - Observer requirements for vessels and plants.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... begin until all previously harvested fish has been offloaded and an observer is aboard the vessel. (ii) An observer may not be transferred off a catcher vessel until the observer confirms that all fish... observers aboard for each day that the vessel is used to catch or process fish in the Central GOA from May...

  19. Characterization of AfaE adhesins produced by extraintestinal and intestinal human Escherichia coli isolates: PCR assays for detection of Afa adhesins that do or do not recognize Dr blood group antigens.

    PubMed

    Le Bouguénec, C; Lalioui, L; du Merle, L; Jouve, M; Courcoux, P; Bouzari, S; Selvarangan, R; Nowicki, B J; Germani, Y; Andremont, A; Gounon, P; Garcia, M I

    2001-05-01

    Operons of the afa family are expressed by pathogenic Escherichia coli strains associated with intestinal and extraintestinal infections in humans and animals. The recently demonstrated heterogeneity of these operons (L. Lalioui, M. Jouve, P. Gounon, and C. Le Bouguénec, Infect. Immun. 67:5048-5059, 1999) was used to develop a new PCR assay for detecting all the operons of the afa family with a single genetic tool. This PCR approach was validated by investigating three collections of human E. coli isolates originating from the stools of infants with diarrhea (88 strains), the urine of patients with pyelonephritis (97 strains), and the blood of cancer patients (115 strains). The results obtained with this single test and those previously obtained with several PCR assays were closely correlated. The AfaE adhesins encoded by the afa operons are variable, particularly with respect to the primary sequence encoded by the afaE gene. The receptor binding specificities have not been determined for all of these adhesins; some recognize the Dr blood group antigen (Afa/Dr(+) adhesins) on the human decay-accelerating factor (DAF) as a receptor, and others (Afa/Dr(-) adhesins) do not. Thus, the afa operons detected in this study were characterized by subtyping the afaE gene using specific PCRs. In addition, the DAF-binding capacities of as-yet-uncharacterized AfaE adhesins were tested by various cellular approaches. The afaE8 subtype (Afa/Dr(-) adhesin) was found to predominate in afa-positive isolates from sepsis patients (75%); it was frequent in afa-positive pyelonephritis E. coli (55.5%) and absent from diarrhea-associated strains. In contrast, Afa/Dr(+) strains (regardless of the afaE subtype) were associated with both diarrhea (100%) and extraintestinal infections (44 and 25% in afa-positive pyelonephritis and sepsis strains, respectively). These data suggest that there is an association between the subtype of AfaE adhesin and the physiological site of the infection

  20. 25 CFR 1000.276 - Does FTCA apply to a self-governance AFA if FTCA is not referenced in the AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Does FTCA apply to a self-governance AFA if FTCA is not referenced in the AFA? 1000.276 Section 1000.276 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS...-governance AFA if FTCA is not referenced in the AFA? Yes, FTCA applies even if the AFA does not mention it....

  1. 25 CFR 1000.276 - Does FTCA apply to a self-governance AFA if FTCA is not referenced in the AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Does FTCA apply to a self-governance AFA if FTCA is not referenced in the AFA? 1000.276 Section 1000.276 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS...-governance AFA if FTCA is not referenced in the AFA? Yes, FTCA applies even if the AFA does not mention it....

  2. 25 CFR 1000.276 - Does FTCA apply to a self-governance AFA if FTCA is not referenced in the AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Does FTCA apply to a self-governance AFA if FTCA is not referenced in the AFA? 1000.276 Section 1000.276 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS...-governance AFA if FTCA is not referenced in the AFA? Yes, FTCA applies even if the AFA does not mention it....

  3. 25 CFR 1000.276 - Does FTCA apply to a self-governance AFA if FTCA is not referenced in the AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Does FTCA apply to a self-governance AFA if FTCA is not referenced in the AFA? 1000.276 Section 1000.276 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS...-governance AFA if FTCA is not referenced in the AFA? Yes, FTCA applies even if the AFA does not mention it....

  4. 25 CFR 1000.276 - Does FTCA apply to a self-governance AFA if FTCA is not referenced in the AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Does FTCA apply to a self-governance AFA if FTCA is not referenced in the AFA? 1000.276 Section 1000.276 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS...-governance AFA if FTCA is not referenced in the AFA? Yes, FTCA applies even if the AFA does not mention it....

  5. Pathogenesis of Afa/Dr diffusely adhering Escherichia coli.

    PubMed

    Servin, Alain L

    2005-04-01

    Over the last few years, dramatic increases in our knowledge about diffusely adhering Escherichia coli (DAEC) pathogenesis have taken place. The typical class of DAEC includes E. coli strains harboring AfaE-I, AfaE-II, AfaE-III, AfaE-V, Dr, Dr-II, F1845, and NFA-I adhesins (Afa/Dr DAEC); these strains (i) have an identical genetic organization and (ii) allow binding to human decay-accelerating factor (DAF) (Afa/Dr(DAF) subclass) or carcinoembryonic antigen (CEA) (Afa/Dr(CEA) subclass). The atypical class of DAEC includes two subclasses of strains; the atypical subclass 1 includes E. coli strains that express AfaE-VII, AfaE-VIII, AAF-I, AAF-II, and AAF-III adhesins, which (i) have an identical genetic organization and (ii) do not bind to human DAF, and the atypical subclass 2 includes E. coli strains that harbor Afa/Dr adhesins or others adhesins promoting diffuse adhesion, together with pathogenicity islands such as the LEE pathogenicity island (DA-EPEC). In this review, the focus is on Afa/Dr DAEC strains that have been found to be associated with urinary tract infections and with enteric infection. The review aims to provide a broad overview and update of the virulence aspects of these intriguing pathogens. Epidemiological studies, diagnostic techniques, characteristic molecular features of Afa/Dr operons, and the respective role of Afa/Dr adhesins and invasins in pathogenesis are described. Following the recognition of membrane-bound receptors, including type IV collagen, DAF, CEACAM1, CEA, and CEACAM6, by Afa/Dr adhesins, activation of signal transduction pathways leads to structural and functional injuries at brush border and junctional domains and to proinflammatory responses in polarized intestinal cells. In addition, uropathogenic Afa/Dr DAEC strains, following recognition of beta(1) integrin as a receptor, enter epithelial cells by a zipper-like, raft- and microtubule-dependent mechanism. Finally, the presence of other, unknown virulence factors and the

  6. Ankyloblepharon filiforme adnatum (AFA) associated with trisomy 18.

    PubMed

    Tüysüz, Beyhan; Ilikkan, Barbaros; Vural, Mehmet; Perk, Yildiz

    2002-01-01

    Ankyloblepharon filiforme adnatum (AFA) is a rare congenital anomaly which is characterized by single or multiple strands joining the upper and lower lids. Its etiology is unknown. A girl with a gestational age of 38 weeks is presented. It was interesting to find an association of AFA with hypoplasia of hair, eyelashes, eyebrows and nails and a karyotype of trisomy 18 in this case.

  7. The National Debate Tournament: A Test of the AFA's Professionalism.

    ERIC Educational Resources Information Center

    Ziegelmueller, George; Baron, Chris

    2000-01-01

    Notes that for two decades issues related to the National Debate Tournament (NDT) consumed major portions of the American Forensic Association's (AFA) annual business meetings. Reviews the circumstances leading to the AFA's vote to sponsor the NDT; examines the association's role in overseeing the NDT; and judges the significance of the…

  8. Palladium complexes of 6-aminofulvene-2-aldiminate (AFA) ligands.

    PubMed

    Bailey, Philip J; Collins, Anna; Haack, Peter; Parsons, Simon; Rahman, Mahmudur; Smith, Damian; White, Fraser J

    2010-02-14

    Bis(N,N'-2,6-diisopropylphenyl)-6-aminofulvene-2-aldimine (4) has been synthesised and characterised. The synthesis and characterisation of two zwitterionic Pd(ii) complexes [(Ph(2)AFA)Pd(Me)DMAP] (1) and [(Ph(2)AFA)Pd(N,N-dimethylbenzylamine-2-C,N)] (2) are reported. Activation of 1 and 2 for ethene polymerisation with Lewis acids such as BF(3) and B(C(6)F(5))(3) were not successful. Attempted synthesis of halide-bridged dimers of the form [(Ph(2)AFA)Pd(mu-X)](2) resulted in formation of bis-chelated complexes [(Cy(2)AFA)(2)Pd] (3) and [((t)Bu(2)AFA)(2)Pd] (5).

  9. 25 CFR 1000.137 - What funds are included in an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false What funds are included in an AFA? 1000.137 Section 1000... are included in an AFA? Bureaus determine the amount of funding to be included in the AFA using the following principles: (a) 403(b)(2) programs. In general, funds are provided in an AFA to the...

  10. 25 CFR 1000.137 - What funds are included in an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false What funds are included in an AFA? 1000.137 Section 1000... are included in an AFA? Bureaus determine the amount of funding to be included in the AFA using the following principles: (a) 403(b)(2) programs. In general, funds are provided in an AFA to the...

  11. 25 CFR 1000.137 - What funds are included in an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false What funds are included in an AFA? 1000.137 Section 1000... are included in an AFA? Bureaus determine the amount of funding to be included in the AFA using the following principles: (a) 403(b)(2) programs. In general, funds are provided in an AFA to the...

  12. 25 CFR 1000.177 - What happens when the AFA is signed?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false What happens when the AFA is signed? 1000.177 Section... Agreements § 1000.177 What happens when the AFA is signed? (a) After all parties have signed the AFA, a copy is sent to the Tribe/Consortium. (b) The Secretary forwards copies of the AFA to: (1) The...

  13. 25 CFR 1000.177 - What happens when the AFA is signed?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false What happens when the AFA is signed? 1000.177 Section... Agreements § 1000.177 What happens when the AFA is signed? (a) After all parties have signed the AFA, a copy is sent to the Tribe/Consortium. (b) The Secretary forwards copies of the AFA to: (1) The...

  14. 25 CFR 1000.137 - What funds are included in an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false What funds are included in an AFA? 1000.137 Section 1000... are included in an AFA? Bureaus determine the amount of funding to be included in the AFA using the following principles: (a) 403(b)(2) programs. In general, funds are provided in an AFA to the...

  15. 25 CFR 1000.177 - What happens when the AFA is signed?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false What happens when the AFA is signed? 1000.177 Section... Agreements § 1000.177 What happens when the AFA is signed? (a) After all parties have signed the AFA, a copy is sent to the Tribe/Consortium. (b) The Secretary forwards copies of the AFA to: (1) The...

  16. 25 CFR 1000.177 - What happens when the AFA is signed?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false What happens when the AFA is signed? 1000.177 Section... Agreements § 1000.177 What happens when the AFA is signed? (a) After all parties have signed the AFA, a copy is sent to the Tribe/Consortium. (b) The Secretary forwards copies of the AFA to: (1) The...

  17. 25 CFR 1000.137 - What funds are included in an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false What funds are included in an AFA? 1000.137 Section 1000... are included in an AFA? Bureaus determine the amount of funding to be included in the AFA using the following principles: (a) 403(b)(2) programs. In general, funds are provided in an AFA to the...

  18. 25 CFR 1000.177 - What happens when the AFA is signed?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false What happens when the AFA is signed? 1000.177 Section... Agreements § 1000.177 What happens when the AFA is signed? (a) After all parties have signed the AFA, a copy is sent to the Tribe/Consortium. (b) The Secretary forwards copies of the AFA to: (1) The...

  19. Development of fast-release solid catchers for rare isotopes

    NASA Astrophysics Data System (ADS)

    Nolen, Jerry; Greene, John; Elam, Jeffrey; Mane, Anil; Sampathkumaran, Uma; Winter, Raymond; Hess, David; Mushfiq, Mohammad; Stracener, Daniel; Wiendenhoever, Ingo

    2015-04-01

    Porous solid catchers of rare isotopes are being developed for use at high power heavy ion accelerator facilities such as RIKEN, FRIB, and RISP. Compact solid catchers are complementary to helium gas catchers for parasitic harvesting of rare isotopes in the in-flight separators. They are useful for short lived isotopes for basic nuclear physics research and longer-lived isotopes for off-line applications. Solid catchers can operate effectively with high intensity secondary beams, e.g. >> 1E10 atoms/s with release times as short as 10-100 milliseconds. A new method using a very sensitive and efficient RGA has been commissioned off-line at Argonne and is currently being shipped to Florida State University for in-beam measurements of the release curves using stable beams. The same porous solid catcher technology is also being evaluated for use in targets for the production of medical isotopes such as 211-At. Research supported by the U.S. DOE Office of Nuclear Physics under the SBIR Program and Contract # DE-AC02-06CH11357 and a University of Chicago Comprehensive Cancer Center/ANL Pilot Project.

  20. Effects of Game Performance on Softball Pitchers and Catchers.

    PubMed

    Oliver, Gretchen D; Plummer, Hillary A; Washington, Jessie; Weimar, Wendi H; Brambeck, Allison

    2017-02-21

    The purpose of this study was to examine the effects of game exposure on pitchers and catchers' hip and glenohumeral range of motion (ROM), isometric strength, and vertical jump performance and power. Five college female softball pitchers (174.24 ± 7.53 cm; 82.10 ± 11.27 kg; 20 ± 1.82 years) and four catchers (165.10 ± 9.04 kg; 68.61 ± 3.87 cm; 20 ± 1.41 years) participated. Hip and glenohumeral rotational ROM; hip and glenohumeral rotational isometric strength; hip abduction and adduction strength; and vertical jump height were assessed pre and post-game exposure. The catchers displayed significantly greater throwing side hip IR ROM (Mean Difference = +6.88°, p=0.03) post-game exposure, while the pitchers exhibited less IR (Mean Difference = -2.02%, p = .013) and ER (Mean Difference = -1.95%, p = .026) strength in the non-throwing side hip pre versus post-game exposure. The performance demands of pitchers and catchers vary greatly, causing position specific decreases/increases in various tests following game performance. Pitchers exhibited significant decreases in non-throwing side hip IR and ER strength. In catchers, throwing side hip IR range of motion significantly increased following game exposure. Further evaluation of ROM, strength, and power is necessary as it is unknown how these changes following a game affect sports performance or risk of injury.

  1. 25 CFR 1000.432 - To whom may a Tribe appeal a decision made before the AFA or an amendment to the AFA or compact...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false To whom may a Tribe appeal a decision made before the AFA or an amendment to the AFA or compact is signed? 1000.432 Section 1000.432 Indians OFFICE OF THE... whom may a Tribe appeal a decision made before the AFA or an amendment to the AFA or compact is...

  2. 25 CFR 1000.432 - To whom may a Tribe appeal a decision made before the AFA or an amendment to the AFA or compact...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false To whom may a Tribe appeal a decision made before the AFA or an amendment to the AFA or compact is signed? 1000.432 Section 1000.432 Indians OFFICE OF THE... whom may a Tribe appeal a decision made before the AFA or an amendment to the AFA or compact is...

  3. 25 CFR 1000.432 - To whom may a Tribe appeal a decision made before the AFA or an amendment to the AFA or compact...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false To whom may a Tribe appeal a decision made before the AFA or an amendment to the AFA or compact is signed? 1000.432 Section 1000.432 Indians OFFICE OF THE... whom may a Tribe appeal a decision made before the AFA or an amendment to the AFA or compact is...

  4. 25 CFR 1000.432 - To whom may a Tribe appeal a decision made before the AFA or an amendment to the AFA or compact...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false To whom may a Tribe appeal a decision made before the AFA or an amendment to the AFA or compact is signed? 1000.432 Section 1000.432 Indians OFFICE OF THE... whom may a Tribe appeal a decision made before the AFA or an amendment to the AFA or compact is...

  5. 25 CFR 1000.432 - To whom may a Tribe appeal a decision made before the AFA or an amendment to the AFA or compact...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false To whom may a Tribe appeal a decision made before the AFA or an amendment to the AFA or compact is signed? 1000.432 Section 1000.432 Indians OFFICE OF THE... whom may a Tribe appeal a decision made before the AFA or an amendment to the AFA or compact is...

  6. The Independent School Admissions Director as Tim Wakefield's Catcher

    ERIC Educational Resources Information Center

    Vachow, Mike

    2012-01-01

    Bob Uecker, the Milwaukee Brewers' quipping radio broadcaster, makes his brief and lackluster career as a major league catcher the subject of much of his humor. About catching the knuckleball, Uecker famously said, "I always thought the knuckleball was the easiest pitch to catch." Of course, there is an even better way to catch a knuckleball, and…

  7. Engineering Protein Hydrogels Using SpyCatcher-SpyTag Chemistry.

    PubMed

    Gao, Xiaoye; Fang, Jie; Xue, Bin; Fu, Linglan; Li, Hongbin

    2016-09-12

    Constructing hydrogels from engineered proteins has attracted significant attention within the material sciences, owing to their myriad potential applications in biomedical engineering. Developing efficient methods to cross-link tailored protein building blocks into hydrogels with desirable mechanical, physical, and functional properties is of paramount importance. By making use of the recently developed SpyCatcher-SpyTag chemistry, we successfully engineered protein hydrogels on the basis of engineered tandem modular elastomeric proteins. Our resultant protein hydrogels are soft but stable, and show excellent biocompatibility. As the first step, we tested the use of these hydrogels as a drug carrier, as well as in encapsulating human lung fibroblast cells. Our results demonstrate the robustness of the SpyCatcher-SpyTag chemistry, even when the SpyTag (or SpyCatcher) is flanked by folded globular domains. These results demonstrate that SpyCatcher-SpyTag chemistry can be used to engineer protein hydrogels from tandem modular elastomeric proteins that can find applications in tissue engineering, in fundamental mechano-biological studies, and as a controlled drug release vehicle.

  8. SCIENTIST DEMONSTRATES PLACING A "RABBIT CATCHER" INTO ONE OF THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SCIENTIST DEMONSTRATES PLACING A "RABBIT CATCHER" INTO ONE OF THE VERTICAL TEST HOLES AT THE TOP OF THE MTR. CONTROL ROD DRIVES ARE BEHIND HIM TOWARDS LEFT OF VIEW. INL NEGATIVE NO. 56-513. Jack L. Anderson, Photographer, 2/13/1956 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  9. 75 FR 6588 - Fisheries of the Exclusive Economic Zone Off Alaska; Pollock for American Fisheries Act Catcher...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-10

    ...; closure. SUMMARY: NMFS is prohibiting directed fishing for pollock by American Fisheries Act (AFA) trawl... TAC allocated to the AFA inshore open access fishery in the BSAI is 2,762 metric tons (mt) as...://alaskafisheries.noaa.gov/sustainablefisheries/afa/afa_sf.htm . In accordance with Sec. 679.20(d)(1)(i),...

  10. 77 FR 3956 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Non-American Fisheries Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-26

    .../ Processors Using Pot Gear in the Western Regulatory Area of the Gulf of Alaska AGENCY: National Marine...- American Fisheries Act (AFA) crab vessels operating as catcher/ processors using pot gear in the Western... as catcher/processors using pot gear in the Western Regulatory Area of the GOA. DATES: Effective...

  11. Recognition of the cellular beta1-chain integrin by the bacterial AfaD invasin is implicated in the internalization of afa-expressing pathogenic Escherichia coli strains.

    PubMed

    Plançon, Laure; Du Merle, Laurence; Le Friec, Sandrine; Gounon, Pierre; Jouve, Mabel; Guignot, Julie; Servin, Alain; Le Bouguénec, Chantal

    2003-10-01

    The afa operons from Escherichia coli associated with extra-intestinal and intestinal infections have been characterized and the AfaD protein has been shown to be involved in the low internalization of laboratory strains expressing the afa-3 operon. The aim of this study was to determine the role of the AfaD invasin during the interaction of pathogenic E. coli with epithelial cells. We show that AfaD is implicated in the entry of a clinical isolate into both HeLa and undifferentiated Caco-2 cells. Once in the cytoplasm of these cells, the bacteria formed inclusions in which they were able to survive for at least 72 h. Internalization assays using polystyrene beads coated with His6-tagged purified AfaD (rAfaD) demonstrated that this invasin mediates entry into cells derived from various tissues (intestine and urothelium) that are targets for afa-positive strains. Consistent with the previous observation that an antibody blockade involving anti-alpha5beta1 integrin abolishes bacterial internalization, we show here that the entry of rAfaD-coated beads was dependent on the production and accessibility of beta1 integrins on the cells. The AfaD proteins belong to a family of invasins that are at least 45% identical. Despite their differences, the recombinant rAfaD-III and rAfaD-VIII proteins both bound to beta1 integrins. Our results suggest that beta1 integrin is a common receptor for AfaD invasins and that additional AfaD-type-specific receptors exist.

  12. Kinetic Controlled Tag-Catcher Interactions for Directed Covalent Protein Assembly

    PubMed Central

    Tan, Lee Ling; Hoon, Shawn S.

    2016-01-01

    Over the last few years, a number of different protein assembly strategies have been developed, greatly expanding the toolbox for controlling macromolecular assembly. One of the most promising developments is a rapid protein ligation approach using a short polypeptide SpyTag and its partner, SpyCatcher derived from Streptococcus pyogenes fibronectin-binding protein, FbaB. To extend this technology, we have engineered and characterized a new Tag-Catcher pair from a related fibronectin-binding protein in Streptococcus dysgalactiae. The polypeptide Tag, named SdyTag, was constructed based on the native Cna protein B-type (CnaB) domain and was found to be highly unreactive to SpyCatcher. SpyCatcher has 320-fold specificity for its native SpyTag compared to SdyTag. Similarly, SdyTag has a 75-fold specificity for its optimized Catcher, named SdyCatcherDANG short, compared to SpyCatcher. These Tag-Catcher pairs were used in combination to demonstrate specific sequential assembly of tagged proteins in vitro. We also demonstrated that the in vivo generation of circularized proteins in a Tag-Catcher specific manner where specific Tags can be left unreacted for use in subsequent ligation reactions. From the success of these experiments, we foresee the application of SdyTags and SpyTags, not only, for multiplexed control of protein assembly but also for the construction of novel protein architectures. PMID:27783674

  13. 25 CFR 1000.86 - What types of programs may be included in an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false What types of programs may be included in an AFA? 1000.86... Programs May Be Included in An Afa § 1000.86 What types of programs may be included in an AFA? A Tribe/Consortium may include in its AFA programs administered by BIA, without regard to the BIA agency or...

  14. 25 CFR 1000.86 - What types of programs may be included in an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false What types of programs may be included in an AFA? 1000.86... Programs May Be Included in An Afa § 1000.86 What types of programs may be included in an AFA? A Tribe/Consortium may include in its AFA programs administered by BIA, without regard to the BIA agency or...

  15. 25 CFR 1000.86 - What types of programs may be included in an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false What types of programs may be included in an AFA? 1000.86... Programs May Be Included in An Afa § 1000.86 What types of programs may be included in an AFA? A Tribe/Consortium may include in its AFA programs administered by BIA, without regard to the BIA agency or...

  16. 25 CFR 1000.86 - What types of programs may be included in an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false What types of programs may be included in an AFA? 1000.86... Programs May Be Included in An Afa § 1000.86 What types of programs may be included in an AFA? A Tribe/Consortium may include in its AFA programs administered by BIA, without regard to the BIA agency or...

  17. 25 CFR 1000.86 - What types of programs may be included in an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false What types of programs may be included in an AFA? 1000.86... Programs May Be Included in An Afa § 1000.86 What types of programs may be included in an AFA? A Tribe/Consortium may include in its AFA programs administered by BIA, without regard to the BIA agency or...

  18. 25 CFR 1000.83 - Can additional provisions be included in an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Can additional provisions be included in an AFA? 1000.83... Scope of Annual Funding Agreements § 1000.83 Can additional provisions be included in an AFA? Yes, any provision that the parties mutually agreed upon may be included in an AFA....

  19. 25 CFR 1000.83 - Can additional provisions be included in an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Can additional provisions be included in an AFA? 1000.83... Scope of Annual Funding Agreements § 1000.83 Can additional provisions be included in an AFA? Yes, any provision that the parties mutually agreed upon may be included in an AFA....

  20. 25 CFR 1000.92 - What funds may not be included in an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false What funds may not be included in an AFA? 1000.92 Section... EDUCATION ACT Annual Funding Agreements for Bureau of Indian Affairs Programs Determining Afa Amounts § 1000.92 What funds may not be included in an AFA? Funds associated with programs prohibited from...

  1. 25 CFR 1000.244 - May the Secretary suspend construction activities under an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... an AFA? 1000.244 Section 1000.244 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... construction activities under an AFA? (a) The Secretary may require a Tribe/Consortium to suspend certain work under a construction portion of an AFA for up to 30 days only if: (1) Site conditions adversely...

  2. 25 CFR 1000.244 - May the Secretary suspend construction activities under an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... an AFA? 1000.244 Section 1000.244 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... construction activities under an AFA? (a) The Secretary may require a Tribe/Consortium to suspend certain work under a construction portion of an AFA for up to 30 days only if: (1) Site conditions adversely...

  3. 25 CFR 1000.92 - What funds may not be included in an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false What funds may not be included in an AFA? 1000.92 Section... EDUCATION ACT Annual Funding Agreements for Bureau of Indian Affairs Programs Determining Afa Amounts § 1000.92 What funds may not be included in an AFA? Funds associated with programs prohibited from...

  4. 25 CFR 1000.82 - What types of provisions must be included in a BIA AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false What types of provisions must be included in a BIA AFA... Scope of Annual Funding Agreements § 1000.82 What types of provisions must be included in a BIA AFA? Each AFA must specify the programs and it must also specify the applicable funding: (a) Retained by...

  5. 25 CFR 1000.83 - Can additional provisions be included in an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Can additional provisions be included in an AFA? 1000.83... Scope of Annual Funding Agreements § 1000.83 Can additional provisions be included in an AFA? Yes, any provision that the parties mutually agreed upon may be included in an AFA....

  6. 25 CFR 1000.82 - What types of provisions must be included in a BIA AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false What types of provisions must be included in a BIA AFA... Scope of Annual Funding Agreements § 1000.82 What types of provisions must be included in a BIA AFA? Each AFA must specify the programs and it must also specify the applicable funding: (a) Retained by...

  7. 25 CFR 1000.178 - When does the AFA become effective?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false When does the AFA become effective? 1000.178 Section 1000... Agreements § 1000.178 When does the AFA become effective? The effective date is not earlier than 90 days after the AFA is submitted to the Congressional committees under § 1000.177(b)....

  8. 25 CFR 1000.178 - When does the AFA become effective?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false When does the AFA become effective? 1000.178 Section 1000... Agreements § 1000.178 When does the AFA become effective? The effective date is not earlier than 90 days after the AFA is submitted to the Congressional committees under § 1000.177(b)....

  9. 25 CFR 1000.178 - When does the AFA become effective?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false When does the AFA become effective? 1000.178 Section 1000... Agreements § 1000.178 When does the AFA become effective? The effective date is not earlier than 90 days after the AFA is submitted to the Congressional committees under § 1000.177(b)....

  10. 25 CFR 1000.244 - May the Secretary suspend construction activities under an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... an AFA? 1000.244 Section 1000.244 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... construction activities under an AFA? (a) The Secretary may require a Tribe/Consortium to suspend certain work under a construction portion of an AFA for up to 30 days only if: (1) Site conditions adversely...

  11. 25 CFR 1000.178 - When does the AFA become effective?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false When does the AFA become effective? 1000.178 Section 1000... Agreements § 1000.178 When does the AFA become effective? The effective date is not earlier than 90 days after the AFA is submitted to the Congressional committees under § 1000.177(b)....

  12. 25 CFR 1000.244 - May the Secretary suspend construction activities under an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... an AFA? 1000.244 Section 1000.244 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... construction activities under an AFA? (a) The Secretary may require a Tribe/Consortium to suspend certain work under a construction portion of an AFA for up to 30 days only if: (1) Site conditions adversely...

  13. 25 CFR 1000.92 - What funds may not be included in an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false What funds may not be included in an AFA? 1000.92 Section... EDUCATION ACT Annual Funding Agreements for Bureau of Indian Affairs Programs Determining Afa Amounts § 1000.92 What funds may not be included in an AFA? Funds associated with programs prohibited from...

  14. 25 CFR 1000.83 - Can additional provisions be included in an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Can additional provisions be included in an AFA? 1000.83... Scope of Annual Funding Agreements § 1000.83 Can additional provisions be included in an AFA? Yes, any provision that the parties mutually agreed upon may be included in an AFA....

  15. 25 CFR 1000.82 - What types of provisions must be included in a BIA AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false What types of provisions must be included in a BIA AFA... Scope of Annual Funding Agreements § 1000.82 What types of provisions must be included in a BIA AFA? Each AFA must specify the programs and it must also specify the applicable funding: (a) Retained by...

  16. 25 CFR 1000.178 - When does the AFA become effective?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false When does the AFA become effective? 1000.178 Section 1000... Agreements § 1000.178 When does the AFA become effective? The effective date is not earlier than 90 days after the AFA is submitted to the Congressional committees under § 1000.177(b)....

  17. 25 CFR 1000.244 - May the Secretary suspend construction activities under an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... an AFA? 1000.244 Section 1000.244 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... construction activities under an AFA? (a) The Secretary may require a Tribe/Consortium to suspend certain work under a construction portion of an AFA for up to 30 days only if: (1) Site conditions adversely...

  18. 25 CFR 1000.92 - What funds may not be included in an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false What funds may not be included in an AFA? 1000.92 Section... EDUCATION ACT Annual Funding Agreements for Bureau of Indian Affairs Programs Determining Afa Amounts § 1000.92 What funds may not be included in an AFA? Funds associated with programs prohibited from...

  19. 25 CFR 1000.92 - What funds may not be included in an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false What funds may not be included in an AFA? 1000.92 Section... EDUCATION ACT Annual Funding Agreements for Bureau of Indian Affairs Programs Determining Afa Amounts § 1000.92 What funds may not be included in an AFA? Funds associated with programs prohibited from...

  20. 25 CFR 1000.82 - What types of provisions must be included in a BIA AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false What types of provisions must be included in a BIA AFA... Scope of Annual Funding Agreements § 1000.82 What types of provisions must be included in a BIA AFA? Each AFA must specify the programs and it must also specify the applicable funding: (a) Retained by...

  1. 25 CFR 1000.83 - Can additional provisions be included in an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Can additional provisions be included in an AFA? 1000.83... Scope of Annual Funding Agreements § 1000.83 Can additional provisions be included in an AFA? Yes, any provision that the parties mutually agreed upon may be included in an AFA....

  2. 25 CFR 1000.82 - What types of provisions must be included in a BIA AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false What types of provisions must be included in a BIA AFA... Scope of Annual Funding Agreements § 1000.82 What types of provisions must be included in a BIA AFA? Each AFA must specify the programs and it must also specify the applicable funding: (a) Retained by...

  3. An inductively heated hot cavity catcher laser ion source

    NASA Astrophysics Data System (ADS)

    Reponen, M.; Moore, I. D.; Pohjalainen, I.; Rothe, S.; Savonen, M.; Sonnenschein, V.; Voss, A.

    2015-12-01

    An inductively heated hot cavity catcher has been constructed for the production of low-energy ion beams of exotic, neutron-deficient Ag isotopes. A proof-of-principle experiment has been realized by implanting primary 107Ag21+ ions from a heavy-ion cyclotron into a graphite catcher. A variable-thickness nickel foil was used to degrade the energy of the primary beam in order to mimic the implantation depth expected from the heavy-ion fusion-evaporation recoils of N = Z 94Ag. Following implantation, the silver atoms diffused out of the graphite and effused into the catcher cavity and transfer tube, where they were resonantly laser ionized using a three-step excitation and ionization scheme. Following mass separation, the ions were identified by scanning the frequency of the first resonant excitation step while recording the ion count rate. Ion release time profiles were measured for different implantation depths and cavity temperatures with the mean delay time varying from 10 to 600 ms. In addition, the diffusion coefficients for silver in graphite were measured for temperatures of 1470 K, 1630 K, and 1720 K, from which an activation energy of 3.2 ± 0.3 eV could be determined.

  4. An inductively heated hot cavity catcher laser ion source

    SciTech Connect

    Reponen, M.; Moore, I. D. Pohjalainen, I.; Savonen, M.; Voss, A.; Rothe, S.; Sonnenschein, V.

    2015-12-15

    An inductively heated hot cavity catcher has been constructed for the production of low-energy ion beams of exotic, neutron-deficient Ag isotopes. A proof-of-principle experiment has been realized by implanting primary {sup 107}Ag{sup 21+} ions from a heavy-ion cyclotron into a graphite catcher. A variable-thickness nickel foil was used to degrade the energy of the primary beam in order to mimic the implantation depth expected from the heavy-ion fusion-evaporation recoils of N = Z {sup 94}Ag. Following implantation, the silver atoms diffused out of the graphite and effused into the catcher cavity and transfer tube, where they were resonantly laser ionized using a three-step excitation and ionization scheme. Following mass separation, the ions were identified by scanning the frequency of the first resonant excitation step while recording the ion count rate. Ion release time profiles were measured for different implantation depths and cavity temperatures with the mean delay time varying from 10 to 600 ms. In addition, the diffusion coefficients for silver in graphite were measured for temperatures of 1470 K, 1630 K, and 1720 K, from which an activation energy of 3.2 ± 0.3 eV could be determined.

  5. The afimbrial adhesive sheath encoded by the afa-3 gene cluster of pathogenic Escherichia coli is composed of two adhesins.

    PubMed

    Garcia, M I; Gounon, P; Courcoux, P; Labigne, A; Le Bouguenec, C

    1996-02-01

    The afa-3 gene cluster determines the formation of an afimbrial adhesive sheath that is expressed by uropathogenic as well as diarrhoea-associated Escherichia coli strains. It contains six genes (afaA-afaF), among which the afaE3 gene is known to code for the structural AfaE-III adhesin (previously designated AFA-III), whereas no role has yet been identified for the afaD gene product. The afa-3 gene cluster is closely related to the daa operon that codes for an adhesin, the F1845 adhesin, which is highly related to the AfaE-III adhesin; however, unlike the AfaE-III adhesin, F1845 is a fimbrial adhesin. Reported in this work is the construction of chimeras between the afa-3 and daa operons. Analyses of the phenotypes conferred by these afa-3/daa chimeric clusters allowed us to conclude that the biogenesis of a fimbrial or an afimbrial adhesin is fully determined by the amino acid sequence of the AfaE-III and F1845 adhesins. Moreover, the role of the AfaD product in the biosynthesis of the afimbrial sheath was assessed by immunogold and immunofluorescence experiments. The AfaD and the AfaE-III products were purified and used to raise rabbit and mouse antisera. Similar to AfaE-III, AfaD was found to be a surface-exposed protein as well as an adhesin; both AfaD and AfaE-III are concomittantly expressed by the bacterial cell. These results demonstrate, for the first time, that the afimbrial adhesive sheath expressed by pathogenic E. coli is composed of two adhesins.

  6. 25 CFR 1000.87 - How does the AFA specify the services provided, functions performed, and responsibilities assumed...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false How does the AFA specify the services provided, functions... Programs May Be Included in An Afa § 1000.87 How does the AFA specify the services provided, functions... AFA must specify in writing the services, functions, and responsibilities to be assumed by the...

  7. 25 CFR 1000.243 - What special provisions must be included in an AFA that contains a construction program?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false What special provisions must be included in an AFA that... must be included in an AFA that contains a construction program? An AFA that contains a construction program must address the requirements listed in this section. (a) The AFA must specify how the...

  8. 25 CFR 1000.243 - What special provisions must be included in an AFA that contains a construction program?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false What special provisions must be included in an AFA that... must be included in an AFA that contains a construction program? An AFA that contains a construction program must address the requirements listed in this section. (a) The AFA must specify how the...

  9. 25 CFR 1000.243 - What special provisions must be included in an AFA that contains a construction program?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false What special provisions must be included in an AFA that... must be included in an AFA that contains a construction program? An AFA that contains a construction program must address the requirements listed in this section. (a) The AFA must specify how the...

  10. 25 CFR 1000.87 - How does the AFA specify the services provided, functions performed, and responsibilities assumed...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false How does the AFA specify the services provided, functions... Programs May Be Included in An Afa § 1000.87 How does the AFA specify the services provided, functions... AFA must specify in writing the services, functions, and responsibilities to be assumed by the...

  11. 25 CFR 1000.243 - What special provisions must be included in an AFA that contains a construction program?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false What special provisions must be included in an AFA that... must be included in an AFA that contains a construction program? An AFA that contains a construction program must address the requirements listed in this section. (a) The AFA must specify how the...

  12. 25 CFR 1000.243 - What special provisions must be included in an AFA that contains a construction program?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false What special provisions must be included in an AFA that... must be included in an AFA that contains a construction program? An AFA that contains a construction program must address the requirements listed in this section. (a) The AFA must specify how the...

  13. 25 CFR 1000.87 - How does the AFA specify the services provided, functions performed, and responsibilities assumed...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false How does the AFA specify the services provided, functions... Programs May Be Included in An Afa § 1000.87 How does the AFA specify the services provided, functions... AFA must specify in writing the services, functions, and responsibilities to be assumed by the...

  14. 25 CFR 1000.87 - How does the AFA specify the services provided, functions performed, and responsibilities assumed...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false How does the AFA specify the services provided, functions... Programs May Be Included in An Afa § 1000.87 How does the AFA specify the services provided, functions... AFA must specify in writing the services, functions, and responsibilities to be assumed by the...

  15. 25 CFR 1000.87 - How does the AFA specify the services provided, functions performed, and responsibilities assumed...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false How does the AFA specify the services provided, functions... Programs May Be Included in An Afa § 1000.87 How does the AFA specify the services provided, functions... AFA must specify in writing the services, functions, and responsibilities to be assumed by the...

  16. Diffusely adherent Escherichia coli strains expressing Afa/Dr adhesins (Afa/Dr DAEC): hitherto unrecognized pathogens.

    PubMed

    Le Bouguénec, Chantal; Servin, Alain L

    2006-03-01

    Diffusely adherent Escherichia coli (DAEC) strains are currently considered to constitute a putative sixth group of diarrheagenic E. coli. However, on the basis of their diffuse adherence to HEp-2 and HeLa cells, the detection of afa/dra/daa-related operons encoding this adherence phenotype, and the mobilization of decay-accelerating factor, both commensal and pathogenic strains can be classified as Afa/Dr DAEC isolates. Furthermore, strains associated with diarrheal diseases and strains causing extra-intestinal infections can also be identified as Afa/Dr DAEC strains. Although several cell signaling events that occur after epithelial cells have been infected by Afa/Dr DAEC have been reported, the pathophysiological processes that allow intestinal and extra-intestinal infections to develop are not fully understood. This review focuses on the genetic organization of the afa/dra/daa-related operons and on the virulence factors that trigger cellular responses, some of which are deleterious for the host cells. Finally, this review suggests future lines of research that could help to elucidate these questions.

  17. 75 FR 7403 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Hook...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-19

    ... Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Hook-and-Line Gear in the Bering Sea and... directed fishing for Pacific cod by catcher/processors using hook-and-line gear in the Bering Sea and... allowance of the 2010 Pacific cod total allowable catch (TAC) allocated to catcher/processors using...

  18. 75 FR 38939 - Fisheries of the Economic Exclusive Zone Off Alaska; Deep-Water Species Fishery by Catcher...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-07

    ... Exclusive Zone Off Alaska; Deep-Water Species Fishery by Catcher/Processor Rockfish Cooperatives in the Gulf... for species that comprise the deep-water species fishery by catcher/processor rockfish cooperatives... limit specified for the deep-water species fishery by catcher/processor rockfish cooperatives subject...

  19. 50 CFR 600.1105 - Longline catcher processor subsector of the Bering Sea and Aleutian Islands (BSAI) non-pollock...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Longline catcher processor subsector of... Capacity Reduction Regulations § 600.1105 Longline catcher processor subsector of the Bering Sea and... catcher processor subsector of the Bering Sea and Aleutian Islands (BSAI) non-pollock groundfish...

  20. Afa, a diffuse adherence fibrillar adhesin associated with enteropathogenic Escherichia coli.

    PubMed

    Keller, Rogéria; Ordoñez, Juana G; de Oliveira, Rosana R; Trabulsi, Luiz R; Baldwin, Thomas J; Knutton, Stuart

    2002-05-01

    O55 is one of the most frequent enteropathogenic Escherichia coli (EPEC) O serogroups implicated in infantile diarrhea in developing countries. Multilocus enzyme electrophoresis analysis showed that this serogroup includes two major electrophoretic types (ET), designated ET1 and ET5. ET1 corresponds to typical EPEC, whilst ET5 comprises strains with different combinations of virulence genes, including those for localized adherence (LA) and diffuse adherence (DA). Here we report that ET5 DA strains possess a DA adhesin, designated EPEC Afa. An 11.6-kb chromosomal region including the DA adhesin operon from one O55:H(-) ET5 EPEC strain was sequenced and found to encode a protein with 98% identity to AfaE-1, an adhesin associated with uropathogenic E. coli. Although described as an afimbrial adhesin, we show that both AfaE-1 and EPEC Afa possess fine fibrillar structures. This is the first characterization and demonstration of an Afa adhesin associated with EPEC.

  1. 76 FR 58414 - Pacific Cod by Non-American Fisheries Act Crab Vessels Harvesting Pacific Cod for Processing by...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-21

    ... National Oceanic and Atmospheric Administration 50 CFR Part 679 RIN 0648-XA715 Pacific Cod by Non-American Fisheries Act Crab Vessels Harvesting Pacific Cod for Processing by the Inshore Component in the Central... directed fishing for Pacific cod by non- American Fisheries Act (AFA) crab vessels that are subject...

  2. 76 FR 59922 - Pacific Cod by Non-American Fisheries Act Crab Vessels Harvesting Pacific Cod for Processing by...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-28

    ... National Oceanic and Atmospheric Administration 50 CFR Part 679 RIN 0648-XA729 Pacific Cod by Non-American Fisheries Act Crab Vessels Harvesting Pacific Cod for Processing by the Inshore Component in the Western... directed fishing for Pacific cod by non- American Fisheries Act (AFA) crab vessels that are subject...

  3. Quantitative analysis of kinematics and kinetics of catchers throwing to second base.

    PubMed

    Plummer, Hillary; Oliver, Gretchen Dawn

    2013-01-01

    The catcher has the most demanding position in the games of baseball and softball with no regulations on how many throws they make during game. It was the purpose of this study to describe the kinematics and kinetics of the throwing motion in catchers when throwing down to second base. It was hypothesised that younger and older catchers would display significantly different throwing kinematics and kinetics. Thirty-eight baseball and softball catchers volunteered to participate. Twenty participants were considered younger (aged 9-14, 10.95 ± 1.76 years, 151.11 ± 15.64 cm, 47.94 ± 18.84 kg) and 18 were deemed the older group (aged 15-23, 18.11 ± 2.61 years, 170.91 ± 8.67 cm, 74.88 ± 10.74 kg). Participants received a pitch and completed five accurate throws to second base in full catching gear. The average ball speed of the older catchers was 21 ± 3.58 meters per second (47 ± 8.02 mph) while the younger catchers averaged 17.2 ± 4.0 meters per second (38.6 ± 8.96 mph). Older catchers had greater shoulder elevation at ball release and significantly greater shoulder external rotation at foot contact and shoulder maximum external rotation than younger catchers. It is clear that chronological age plays a role in the throwing mechanics observed in catchers throwing down to second base, however the effects of these differences are not fully understood (i.e., skeletal maturity, experience, strength).

  4. Pathogenesis of human diffusely adhering Escherichia coli expressing Afa/Dr adhesins (Afa/Dr DAEC): current insights and future challenges.

    PubMed

    Servin, Alain L

    2014-10-01

    The pathogenicity and clinical pertinence of diffusely adhering Escherichia coli expressing the Afa/Dr adhesins (Afa/Dr DAEC) in urinary tract infections (UTIs) and pregnancy complications are well established. In contrast, the implication of intestinal Afa/Dr DAEC in diarrhea is still under debate. These strains are age dependently involved in diarrhea in children, are apparently not involved in diarrhea in adults, and can also be asymptomatic intestinal microbiota strains in children and adult. This comprehensive review analyzes the epidemiology and diagnosis and highlights recent progress which has improved the understanding of Afa/Dr DAEC pathogenesis. Here, I summarize the roles of Afa/Dr DAEC virulence factors, including Afa/Dr adhesins, flagella, Sat toxin, and pks island products, in the development of specific mechanisms of pathogenicity. In intestinal epithelial polarized cells, the Afa/Dr adhesins trigger cell membrane receptor clustering and activation of the linked cell signaling pathways, promote structural and functional cell lesions and injuries in intestinal barrier, induce proinflammatory responses, create angiogenesis, instigate epithelial-mesenchymal transition-like events, and lead to pks-dependent DNA damage. UTI-associated Afa/Dr DAEC strains, following adhesin-membrane receptor cell interactions and activation of associated lipid raft-dependent cell signaling pathways, internalize in a microtubule-dependent manner within urinary tract epithelial cells, develop a particular intracellular lifestyle, and trigger a toxin-dependent cell detachment. In response to Afa/Dr DAEC infection, the host epithelial cells generate antibacterial defense responses. Finally, I discuss a hypothetical role of intestinal Afa/Dr DAEC strains that can act as "silent pathogens" with the capacity to emerge as "pathobionts" for the development of inflammatory bowel disease and intestinal carcinogenesis.

  5. Pathogenesis of Human Diffusely Adhering Escherichia coli Expressing Afa/Dr Adhesins (Afa/Dr DAEC): Current Insights and Future Challenges

    PubMed Central

    2014-01-01

    SUMMARY The pathogenicity and clinical pertinence of diffusely adhering Escherichia coli expressing the Afa/Dr adhesins (Afa/Dr DAEC) in urinary tract infections (UTIs) and pregnancy complications are well established. In contrast, the implication of intestinal Afa/Dr DAEC in diarrhea is still under debate. These strains are age dependently involved in diarrhea in children, are apparently not involved in diarrhea in adults, and can also be asymptomatic intestinal microbiota strains in children and adult. This comprehensive review analyzes the epidemiology and diagnosis and highlights recent progress which has improved the understanding of Afa/Dr DAEC pathogenesis. Here, I summarize the roles of Afa/Dr DAEC virulence factors, including Afa/Dr adhesins, flagella, Sat toxin, and pks island products, in the development of specific mechanisms of pathogenicity. In intestinal epithelial polarized cells, the Afa/Dr adhesins trigger cell membrane receptor clustering and activation of the linked cell signaling pathways, promote structural and functional cell lesions and injuries in intestinal barrier, induce proinflammatory responses, create angiogenesis, instigate epithelial-mesenchymal transition-like events, and lead to pks-dependent DNA damage. UTI-associated Afa/Dr DAEC strains, following adhesin-membrane receptor cell interactions and activation of associated lipid raft-dependent cell signaling pathways, internalize in a microtubule-dependent manner within urinary tract epithelial cells, develop a particular intracellular lifestyle, and trigger a toxin-dependent cell detachment. In response to Afa/Dr DAEC infection, the host epithelial cells generate antibacterial defense responses. Finally, I discuss a hypothetical role of intestinal Afa/Dr DAEC strains that can act as “silent pathogens” with the capacity to emerge as “pathobionts” for the development of inflammatory bowel disease and intestinal carcinogenesis. PMID:25278576

  6. Immunocytochemistry of the AfaE adhesin and AfaD invasin produced by pathogenic Escherichia coli strains during interaction of the bacteria with HeLa cells by high-resolution scanning electron microscopy.

    PubMed

    Gounon, P; Jouve, M; Le Bouguénec, C

    2000-04-01

    We used a recent scanning electron microscope equipped with field emission gun and highly sensitive detectors to develop a fast and simple protocol for double immunogold staining using 10- and 15-nm gold particles. We used this approach to analyse the afimbrial adhesive sheath produced by pathogenic Escherichia coli interacting with the surface of epithelial cells. We demonstrated that AfaE adhesin and AfaD invasin were exposed at the bacterial surface during the interaction. This method could be easily and widely extended to the study of the early invasion process of many bacterial and viral pathogens, by immunocytochemical probing.

  7. SpyTag/SpyCatcher Cyclization Enhances the Thermostability of Firefly Luciferase

    PubMed Central

    Si, Meng; Xu, Qing

    2016-01-01

    SpyTag can spontaneously form a covalent isopeptide bond with its protein partner SpyCatcher. Firefly luciferase from Photinus pyralis was cyclized in vivo by fusing SpyCatcher at the N terminus and SpyTag at the C terminus. Circular LUC was more thermostable and alkali-tolerant than the wild type, without compromising the specific activity. Structural analysis indicated that the cyclized LUC increased the thermodynamic stability of the structure and remained more properly folded at high temperatures when compared with the wild type. We also prepared an N-terminally and C-terminally shortened form of the SpyCatcher protein and cyclization using this truncated form led to even more thermostability than the original form. Our findings suggest that cyclization with SpyTag and SpyCatcher is a promising and effective strategy to enhance thermostability of enzymes. PMID:27658030

  8. Eastern Band of Cherokee Indians Awarded 2015 EPA Region 4 Rain Catcher Award

    EPA Pesticide Factsheets

    ATLANTA - The Environmental Protection Agency (EPA) recognized the Eastern Band of Cherokee Indians with the regional 2015 EPA Rain Catcher Award in the Tribal Category for the Native Plant Facility project in western North Carolina. The award was g

  9. Mississippi State University Awarded 2015 EPA Region 4 Rain Catcher Award

    EPA Pesticide Factsheets

    ATLANTA - The Environmental Protection Agency (EPA) recognized Mississippi State University with the regional 2015 EPA Rain Catcher Award in the Neighborhood/Community Category for the Oktibbeha County Heritage Museum's rain garden program in Starkv

  10. Nominations are Open for EPA Region 4 Rain Catcher Award-Deadline is March 18, 2016

    EPA Pesticide Factsheets

    ATLANTA - For the third year in a row, the U.S. Environmental Protection Agency (EPA) is accepting nominations for the Rain Catcher Awards for excellence in implementation of stormwater green infrastructure projects during the past year. Award categ

  11. Nominations Open for EPA Region 4 Rain Catcher Award - Deadline is April 17, 2015

    EPA Pesticide Factsheets

    ATLANTA - For the second year in a row, the U.S. Environmental Protection Agency (EPA) is now accepting nominations for the Rain Catcher Awards for excellence in the implementation of stormwater green infrastructure projects during the last ten year

  12. Numerical and Experimental Simulation of a Vertical High Speed Motorcompressor Rotor Drop onto Catcher Bearings

    NASA Astrophysics Data System (ADS)

    Ransom, David; Masala, Andrea; Moore, Jeffrey; Vannini, Giuseppe; Camatti, Massimo

    A new research program was jointly set up between GE Oil&Gas and Southwest Research Institute (SwRI), to predict and test the dynamics of a vertical rotor drop on catcher bearings. A numerical tool able to account for flexible rotor and stator dynamics, catcher bearing stiffness and damping mechanism was developed. An experimental activity on a new vertical rotor test rig was carried out. A first analysis of numerical simulations and experimental analysis is presented in this paper.

  13. Resistance of Concrete Masonry Walls With Membrane Catcher Systems Subjected to Blast Loading

    DTIC Science & Technology

    2010-12-01

    are made. sandwich panel, prestressed concrete , composite, test, explosive load U U U UU 22 Paul Sheppard Reset 1 Resistance of...AFRL-RX-TY-TP-2010-0090 RESISTANCE OF CONCRETE MASONRY WALLS WITH MEMBRANE CATCHER SYSTEMS SUBJECTED TO BLAST LOADING...Include area code) 29-DEC-2010 Refereed Journal Article 01-OCT-2008 -- 31-DEC-2009 Resistance of Concrete Masonry Walls With Membrane Catcher Systems

  14. Presence of pap-, sfa-, and afa-related sequences in necrotoxigenic Escherichia coli isolates from cattle: evidence for new variants of the AFA family.

    PubMed

    Mainil, J G; Jacquemin, E; Hérault, F; Oswald, E

    1997-07-01

    Necrotoxigenic Escherichia coli (NTEC) are associated with intestinal and extraintestinal diseases in animals and human beings and produce Cytotoxic Necrotizing Factor 1 (CNF1) or 2 (CNF2). Fourty-three NTEC1, 42 NTEC2, and 32 CNF-negative isolates from cattle were tested by colony DNA hybridization, by plasmid DNA hybridization and by PCR assays for the presence of DNA sequences homologous to the operons coding for fimbrial (PAP/PRS, SFA/FIC, and F17) and afimbrial (AFA/Dr) adhesins of extraintestinal E. coli. Most NTEC1 isolates hybridized with the PAP probes and either the SFA probe (37%) or the AFA probes (49%). Most NTEC2 isolates, in contrast, hybridized with the F17 probe (45%), the AFA probes (19%), or the F17 and AFA probes (22%). A probe-positive plasmid was identified in each of the 19 NTEC2 isolates studied. They all hybridized with the CNF2 toxin probe (Vir plasmids) and most of them with the F17 (6 plasmids) or AFA (7 plasmids) probes. PCR amplification was obtained with 6 of the 11 NTEC isolates tested for the papGII/prsG genes; with all 5 NTEC isolates tested for the sfa and related operons; but with none of the 18 NTEC isolates tested for the afa and related operons. pap-, sfa-, and afa-related sequences are thus present in NTEC isolates from cattle in addition to f17-related operons and may code for adhesins corresponding to specific colonization factors. f17- and afa-related sequences can be located on the Vir plasmids along with the cnf2 gene. Existence of new variants of the AFA/Dr family is evident from the negative results of this family-specific PCR assay.

  15. 25 CFR 1000.248 - What is the Tribe's/Consortium's role in a construction program included in an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... program included in an AFA? 1000.248 Section 1000.248 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN.../Consortium's role in a construction program included in an AFA? The Tribe/Consortium has the following role regarding a construction portion of an AFA: (a) Under the Act, the Indian Tribe/Consortium must...

  16. 25 CFR 1000.249 - What is the Secretary's role in a construction program in an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... an AFA? 1000.249 Section 1000.249 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... construction program in an AFA? The Secretary has the following role regarding a construction program contained in an AFA: (a) Except as provided in § 1000.256, the Secretary may review and approve planning...

  17. 25 CFR 1000.181 - How does the Tribe/Consortium initiate the negotiation of a successor AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... a successor AFA? 1000.181 Section 1000.181 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... negotiation of a successor AFA? Although a written request is desirable to document the precise request and... change in an existing program in the AFA, it should notify the other party of the change at the...

  18. 25 CFR 1000.405 - Are AFA funds non-Federal funds for the purpose of meeting matching requirements?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Are AFA funds non-Federal funds for the purpose of... AMENDMENTS TO THE INDIAN SELF-DETERMINATION AND EDUCATION ACT Miscellaneous Provisions § 1000.405 Are AFA funds non-Federal funds for the purpose of meeting matching requirements? Yes, self-governance AFA...

  19. 25 CFR 1000.405 - Are AFA funds non-Federal funds for the purpose of meeting matching requirements?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Are AFA funds non-Federal funds for the purpose of... AMENDMENTS TO THE INDIAN SELF-DETERMINATION AND EDUCATION ACT Miscellaneous Provisions § 1000.405 Are AFA funds non-Federal funds for the purpose of meeting matching requirements? Yes, self-governance AFA...

  20. 25 CFR 1000.405 - Are AFA funds non-Federal funds for the purpose of meeting matching requirements?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Are AFA funds non-Federal funds for the purpose of... AMENDMENTS TO THE INDIAN SELF-DETERMINATION AND EDUCATION ACT Miscellaneous Provisions § 1000.405 Are AFA funds non-Federal funds for the purpose of meeting matching requirements? Yes, self-governance AFA...

  1. 25 CFR 1000.248 - What is the Tribe's/Consortium's role in a construction program included in an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... program included in an AFA? 1000.248 Section 1000.248 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN.../Consortium's role in a construction program included in an AFA? The Tribe/Consortium has the following role regarding a construction portion of an AFA: (a) Under the Act, the Indian Tribe/Consortium must...

  2. 25 CFR 1000.242 - What provisions relating to a construction program may be included in an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... included in an AFA? 1000.242 Section 1000.242 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... construction program may be included in an AFA? The Secretary and the Tribe/Consortium may negotiate to apply... to a construction part of an AFA. Absent a negotiated agreement, such provisions and...

  3. 25 CFR 1000.242 - What provisions relating to a construction program may be included in an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... included in an AFA? 1000.242 Section 1000.242 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... construction program may be included in an AFA? The Secretary and the Tribe/Consortium may negotiate to apply... to a construction part of an AFA. Absent a negotiated agreement, such provisions and...

  4. 25 CFR 1000.85 - Can a Tribe/Consortium negotiate an AFA with a term that exceeds one year?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Can a Tribe/Consortium negotiate an AFA with a term that... Programs Contents and Scope of Annual Funding Agreements § 1000.85 Can a Tribe/Consortium negotiate an AFA... availability of Congressional appropriations, a Tribe/Consortium may negotiate an AFA with a term that...

  5. 25 CFR 1000.390 - How can a Tribe/Consortium hire a Federal employee to help implement an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... help implement an AFA? 1000.390 Section 1000.390 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... Tribe/Consortium hire a Federal employee to help implement an AFA? If a Tribe/Consortium chooses to hire... negotiated Federal benefit package that is paid for by the Tribe/Consortium out of AFA program funds; or...

  6. 25 CFR 1000.85 - Can a Tribe/Consortium negotiate an AFA with a term that exceeds one year?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Can a Tribe/Consortium negotiate an AFA with a term that... Programs Contents and Scope of Annual Funding Agreements § 1000.85 Can a Tribe/Consortium negotiate an AFA... availability of Congressional appropriations, a Tribe/Consortium may negotiate an AFA with a term that...

  7. 25 CFR 1000.249 - What is the Secretary's role in a construction program in an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... an AFA? 1000.249 Section 1000.249 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... construction program in an AFA? The Secretary has the following role regarding a construction program contained in an AFA: (a) Except as provided in § 1000.256, the Secretary may review and approve planning...

  8. 25 CFR 1000.91 - What funds must be transferred to a Tribe/Consortium under an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... under an AFA? 1000.91 Section 1000.91 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... Programs Determining Afa Amounts § 1000.91 What funds must be transferred to a Tribe/Consortium under an AFA? (a) At the option of the Tribe/Consortium, the Secretary must provide the following program...

  9. 25 CFR 1000.249 - What is the Secretary's role in a construction program in an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... an AFA? 1000.249 Section 1000.249 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... construction program in an AFA? The Secretary has the following role regarding a construction program contained in an AFA: (a) Except as provided in § 1000.256, the Secretary may review and approve planning...

  10. 25 CFR 1000.405 - Are AFA funds non-Federal funds for the purpose of meeting matching requirements?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Are AFA funds non-Federal funds for the purpose of... AMENDMENTS TO THE INDIAN SELF-DETERMINATION AND EDUCATION ACT Miscellaneous Provisions § 1000.405 Are AFA funds non-Federal funds for the purpose of meeting matching requirements? Yes, self-governance AFA...

  11. 25 CFR 1000.248 - What is the Tribe's/Consortium's role in a construction program included in an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... program included in an AFA? 1000.248 Section 1000.248 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN.../Consortium's role in a construction program included in an AFA? The Tribe/Consortium has the following role regarding a construction portion of an AFA: (a) Under the Act, the Indian Tribe/Consortium must...

  12. 25 CFR 1000.248 - What is the Tribe's/Consortium's role in a construction program included in an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... program included in an AFA? 1000.248 Section 1000.248 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN.../Consortium's role in a construction program included in an AFA? The Tribe/Consortium has the following role regarding a construction portion of an AFA: (a) Under the Act, the Indian Tribe/Consortium must...

  13. 25 CFR 1000.181 - How does the Tribe/Consortium initiate the negotiation of a successor AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... a successor AFA? 1000.181 Section 1000.181 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... negotiation of a successor AFA? Although a written request is desirable to document the precise request and... change in an existing program in the AFA, it should notify the other party of the change at the...

  14. 25 CFR 1000.248 - What is the Tribe's/Consortium's role in a construction program included in an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... program included in an AFA? 1000.248 Section 1000.248 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN.../Consortium's role in a construction program included in an AFA? The Tribe/Consortium has the following role regarding a construction portion of an AFA: (a) Under the Act, the Indian Tribe/Consortium must...

  15. 25 CFR 1000.85 - Can a Tribe/Consortium negotiate an AFA with a term that exceeds one year?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Can a Tribe/Consortium negotiate an AFA with a term that... Programs Contents and Scope of Annual Funding Agreements § 1000.85 Can a Tribe/Consortium negotiate an AFA... availability of Congressional appropriations, a Tribe/Consortium may negotiate an AFA with a term that...

  16. 25 CFR 1000.240 - What construction programs included in an AFA are subject to this subpart?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false What construction programs included in an AFA are subject... in an AFA are subject to this subpart? (a) All BIA and non-BIA construction programs included in an AFA are subject to this subpart. This includes design, construction, repair, improvement,...

  17. 25 CFR 1000.181 - How does the Tribe/Consortium initiate the negotiation of a successor AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... a successor AFA? 1000.181 Section 1000.181 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... negotiation of a successor AFA? Although a written request is desirable to document the precise request and... change in an existing program in the AFA, it should notify the other party of the change at the...

  18. 25 CFR 1000.91 - What funds must be transferred to a Tribe/Consortium under an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... under an AFA? 1000.91 Section 1000.91 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... Programs Determining Afa Amounts § 1000.91 What funds must be transferred to a Tribe/Consortium under an AFA? (a) At the option of the Tribe/Consortium, the Secretary must provide the following program...

  19. 25 CFR 1000.85 - Can a Tribe/Consortium negotiate an AFA with a term that exceeds one year?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Can a Tribe/Consortium negotiate an AFA with a term that... Programs Contents and Scope of Annual Funding Agreements § 1000.85 Can a Tribe/Consortium negotiate an AFA... availability of Congressional appropriations, a Tribe/Consortium may negotiate an AFA with a term that...

  20. 25 CFR 1000.390 - How can a Tribe/Consortium hire a Federal employee to help implement an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... help implement an AFA? 1000.390 Section 1000.390 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... Tribe/Consortium hire a Federal employee to help implement an AFA? If a Tribe/Consortium chooses to hire... negotiated Federal benefit package that is paid for by the Tribe/Consortium out of AFA program funds; or...

  1. 25 CFR 1000.242 - What provisions relating to a construction program may be included in an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... included in an AFA? 1000.242 Section 1000.242 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... construction program may be included in an AFA? The Secretary and the Tribe/Consortium may negotiate to apply... to a construction part of an AFA. Absent a negotiated agreement, such provisions and...

  2. 25 CFR 1000.181 - How does the Tribe/Consortium initiate the negotiation of a successor AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... a successor AFA? 1000.181 Section 1000.181 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... negotiation of a successor AFA? Although a written request is desirable to document the precise request and... change in an existing program in the AFA, it should notify the other party of the change at the...

  3. 25 CFR 1000.242 - What provisions relating to a construction program may be included in an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... included in an AFA? 1000.242 Section 1000.242 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... construction program may be included in an AFA? The Secretary and the Tribe/Consortium may negotiate to apply... to a construction part of an AFA. Absent a negotiated agreement, such provisions and...

  4. 25 CFR 1000.240 - What construction programs included in an AFA are subject to this subpart?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false What construction programs included in an AFA are subject... in an AFA are subject to this subpart? (a) All BIA and non-BIA construction programs included in an AFA are subject to this subpart. This includes design, construction, repair, improvement,...

  5. 25 CFR 1000.390 - How can a Tribe/Consortium hire a Federal employee to help implement an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... help implement an AFA? 1000.390 Section 1000.390 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... Tribe/Consortium hire a Federal employee to help implement an AFA? If a Tribe/Consortium chooses to hire... negotiated Federal benefit package that is paid for by the Tribe/Consortium out of AFA program funds; or...

  6. 25 CFR 1000.91 - What funds must be transferred to a Tribe/Consortium under an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... under an AFA? 1000.91 Section 1000.91 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... Programs Determining Afa Amounts § 1000.91 What funds must be transferred to a Tribe/Consortium under an AFA? (a) At the option of the Tribe/Consortium, the Secretary must provide the following program...

  7. 25 CFR 1000.249 - What is the Secretary's role in a construction program in an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... an AFA? 1000.249 Section 1000.249 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... construction program in an AFA? The Secretary has the following role regarding a construction program contained in an AFA: (a) Except as provided in § 1000.256, the Secretary may review and approve planning...

  8. 25 CFR 1000.240 - What construction programs included in an AFA are subject to this subpart?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false What construction programs included in an AFA are subject... in an AFA are subject to this subpart? (a) All BIA and non-BIA construction programs included in an AFA are subject to this subpart. This includes design, construction, repair, improvement,...

  9. 25 CFR 1000.181 - How does the Tribe/Consortium initiate the negotiation of a successor AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... a successor AFA? 1000.181 Section 1000.181 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... negotiation of a successor AFA? Although a written request is desirable to document the precise request and... change in an existing program in the AFA, it should notify the other party of the change at the...

  10. 25 CFR 1000.240 - What construction programs included in an AFA are subject to this subpart?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false What construction programs included in an AFA are subject... in an AFA are subject to this subpart? (a) All BIA and non-BIA construction programs included in an AFA are subject to this subpart. This includes design, construction, repair, improvement,...

  11. 25 CFR 1000.390 - How can a Tribe/Consortium hire a Federal employee to help implement an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... help implement an AFA? 1000.390 Section 1000.390 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... Tribe/Consortium hire a Federal employee to help implement an AFA? If a Tribe/Consortium chooses to hire... negotiated Federal benefit package that is paid for by the Tribe/Consortium out of AFA program funds; or...

  12. 25 CFR 1000.91 - What funds must be transferred to a Tribe/Consortium under an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... under an AFA? 1000.91 Section 1000.91 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... Programs Determining Afa Amounts § 1000.91 What funds must be transferred to a Tribe/Consortium under an AFA? (a) At the option of the Tribe/Consortium, the Secretary must provide the following program...

  13. 25 CFR 1000.390 - How can a Tribe/Consortium hire a Federal employee to help implement an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... help implement an AFA? 1000.390 Section 1000.390 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... Tribe/Consortium hire a Federal employee to help implement an AFA? If a Tribe/Consortium chooses to hire... negotiated Federal benefit package that is paid for by the Tribe/Consortium out of AFA program funds; or...

  14. 25 CFR 1000.405 - Are AFA funds non-Federal funds for the purpose of meeting matching requirements?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Are AFA funds non-Federal funds for the purpose of... AMENDMENTS TO THE INDIAN SELF-DETERMINATION AND EDUCATION ACT Miscellaneous Provisions § 1000.405 Are AFA funds non-Federal funds for the purpose of meeting matching requirements? Yes, self-governance AFA...

  15. 25 CFR 1000.242 - What provisions relating to a construction program may be included in an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... included in an AFA? 1000.242 Section 1000.242 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... construction program may be included in an AFA? The Secretary and the Tribe/Consortium may negotiate to apply... to a construction part of an AFA. Absent a negotiated agreement, such provisions and...

  16. 25 CFR 1000.85 - Can a Tribe/Consortium negotiate an AFA with a term that exceeds one year?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Can a Tribe/Consortium negotiate an AFA with a term that... Programs Contents and Scope of Annual Funding Agreements § 1000.85 Can a Tribe/Consortium negotiate an AFA... availability of Congressional appropriations, a Tribe/Consortium may negotiate an AFA with a term that...

  17. 25 CFR 1000.249 - What is the Secretary's role in a construction program in an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... an AFA? 1000.249 Section 1000.249 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... construction program in an AFA? The Secretary has the following role regarding a construction program contained in an AFA: (a) Except as provided in § 1000.256, the Secretary may review and approve planning...

  18. 25 CFR 1000.91 - What funds must be transferred to a Tribe/Consortium under an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... under an AFA? 1000.91 Section 1000.91 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... Programs Determining Afa Amounts § 1000.91 What funds must be transferred to a Tribe/Consortium under an AFA? (a) At the option of the Tribe/Consortium, the Secretary must provide the following program...

  19. 25 CFR 1000.240 - What construction programs included in an AFA are subject to this subpart?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false What construction programs included in an AFA are subject... in an AFA are subject to this subpart? (a) All BIA and non-BIA construction programs included in an AFA are subject to this subpart. This includes design, construction, repair, improvement,...

  20. 25 CFR 1000.278 - Does this coverage extend to subcontractors of self-governance AFAs?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Does this coverage extend to subcontractors of self-governance AFAs? 1000.278 Section 1000.278 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... subcontractors of self-governance AFAs? No, subcontractors or subgrantees providing services to a Pub. L....

  1. 25 CFR 1000.397 - Are there any restrictions on how AFA funds may be spent?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Are there any restrictions on how AFA funds may be spent? 1000.397 Section 1000.397 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE...-DETERMINATION AND EDUCATION ACT Miscellaneous Provisions § 1000.397 Are there any restrictions on how AFA...

  2. 25 CFR 1000.182 - What is the process for negotiating a successor AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false What is the process for negotiating a successor AFA? 1000.182 Section 1000.182 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE... Successor Annual Funding Agreements § 1000.182 What is the process for negotiating a successor AFA?...

  3. 25 CFR 1000.397 - Are there any restrictions on how AFA funds may be spent?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Are there any restrictions on how AFA funds may be spent? 1000.397 Section 1000.397 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE...-DETERMINATION AND EDUCATION ACT Miscellaneous Provisions § 1000.397 Are there any restrictions on how AFA...

  4. 78 FR 15705 - Proposed Information Collection; Comment Request; American Fisheries Act (AFA): Permits

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-12

    ... Fisheries Act (AFA): Permits AGENCY: National Oceanic and Atmospheric Administration (NOAA), Commerce... currently approved information collection. In response to the American Fisheries Act (AFA), NMFS developed a management program for Bering Sea and Aleutian Islands Management Area (BSAI) pollock to include a set of...

  5. 25 CFR 1000.397 - Are there any restrictions on how AFA funds may be spent?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Are there any restrictions on how AFA funds may be spent? 1000.397 Section 1000.397 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE...-DETERMINATION AND EDUCATION ACT Miscellaneous Provisions § 1000.397 Are there any restrictions on how AFA...

  6. 25 CFR 1000.332 - Who may retrocede a program in an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Who may retrocede a program in an AFA? 1000.332 Section 1000.332 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ANNUAL... EDUCATION ACT Retrocession § 1000.332 Who may retrocede a program in an AFA? A Tribe/Consortium...

  7. 25 CFR 1000.182 - What is the process for negotiating a successor AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false What is the process for negotiating a successor AFA? 1000.182 Section 1000.182 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE... Successor Annual Funding Agreements § 1000.182 What is the process for negotiating a successor AFA?...

  8. 25 CFR 1000.182 - What is the process for negotiating a successor AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false What is the process for negotiating a successor AFA? 1000.182 Section 1000.182 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE... Successor Annual Funding Agreements § 1000.182 What is the process for negotiating a successor AFA?...

  9. 25 CFR 1000.81 - What is an annual funding agreement (AFA)?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false What is an annual funding agreement (AFA)? 1000.81 Section 1000.81 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR... is an annual funding agreement (AFA)? Annual funding agreements are legally binding and...

  10. 25 CFR 1000.81 - What is an annual funding agreement (AFA)?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false What is an annual funding agreement (AFA)? 1000.81 Section 1000.81 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR... is an annual funding agreement (AFA)? Annual funding agreements are legally binding and...

  11. 25 CFR 1000.332 - Who may retrocede a program in an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Who may retrocede a program in an AFA? 1000.332 Section 1000.332 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ANNUAL... EDUCATION ACT Retrocession § 1000.332 Who may retrocede a program in an AFA? A Tribe/Consortium...

  12. 25 CFR 1000.182 - What is the process for negotiating a successor AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false What is the process for negotiating a successor AFA? 1000.182 Section 1000.182 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE... Successor Annual Funding Agreements § 1000.182 What is the process for negotiating a successor AFA?...

  13. 25 CFR 1000.182 - What is the process for negotiating a successor AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false What is the process for negotiating a successor AFA? 1000.182 Section 1000.182 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE... Successor Annual Funding Agreements § 1000.182 What is the process for negotiating a successor AFA?...

  14. 25 CFR 1000.332 - Who may retrocede a program in an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Who may retrocede a program in an AFA? 1000.332 Section 1000.332 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ANNUAL... EDUCATION ACT Retrocession § 1000.332 Who may retrocede a program in an AFA? A Tribe/Consortium...

  15. 25 CFR 1000.397 - Are there any restrictions on how AFA funds may be spent?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Are there any restrictions on how AFA funds may be spent? 1000.397 Section 1000.397 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE...-DETERMINATION AND EDUCATION ACT Miscellaneous Provisions § 1000.397 Are there any restrictions on how AFA...

  16. Evidence and Ethics in Individual Events: An Examination of an AFA-NIET Final Round.

    ERIC Educational Resources Information Center

    Cronn-Mills, Daniel; Schnoor, Larry G.

    A study investigated the use of source citations and evidence in the final round of Informative Speaking at the 1998 American Forensic Association-National Individual Events Tournament (AFA-NIET). The "AFA Code of Forensics Program and Forensics Tournament Standards for College and Universities" was the framework for the analysis.…

  17. A new case of acromegaloid facial appearance (AFA) syndrome with an expanded phenotype.

    PubMed

    Zelante, L; Gasparini, P; Savoia, A; Lomuto, M; Pellicano, R

    2000-07-01

    A patient presenting with the findings of Acromegaloid Facial Appearance (AFA) syndrome is reported. This case also shows pericardial effusion and skin lesions that both enlarge the spectrum of the phenotype and lump AFA syndrome with another proposed distinct condition [Irvine et al., (1996) J med Genet 33:972-974].

  18. 25 CFR 1000.81 - What is an annual funding agreement (AFA)?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false What is an annual funding agreement (AFA)? 1000.81 Section 1000.81 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR... is an annual funding agreement (AFA)? Annual funding agreements are legally binding and...

  19. 25 CFR 1000.81 - What is an annual funding agreement (AFA)?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false What is an annual funding agreement (AFA)? 1000.81 Section 1000.81 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR... is an annual funding agreement (AFA)? Annual funding agreements are legally binding and...

  20. 25 CFR 1000.332 - Who may retrocede a program in an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Who may retrocede a program in an AFA? 1000.332 Section 1000.332 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ANNUAL... EDUCATION ACT Retrocession § 1000.332 Who may retrocede a program in an AFA? A Tribe/Consortium...

  1. 25 CFR 1000.332 - Who may retrocede a program in an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Who may retrocede a program in an AFA? 1000.332 Section 1000.332 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ANNUAL... EDUCATION ACT Retrocession § 1000.332 Who may retrocede a program in an AFA? A Tribe/Consortium...

  2. 25 CFR 1000.397 - Are there any restrictions on how AFA funds may be spent?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Are there any restrictions on how AFA funds may be spent? 1000.397 Section 1000.397 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE...-DETERMINATION AND EDUCATION ACT Miscellaneous Provisions § 1000.397 Are there any restrictions on how AFA...

  3. 25 CFR 1000.81 - What is an annual funding agreement (AFA)?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false What is an annual funding agreement (AFA)? 1000.81 Section 1000.81 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR... is an annual funding agreement (AFA)? Annual funding agreements are legally binding and...

  4. Adaptive Focused Acoustics (AFA) Improves the Performance of Microtiter Plate ELISAs.

    PubMed

    Green, David J; Rudd, Edwin A; Laugharn, James A

    2014-08-01

    We investigated the use of Adaptive Focused Acoustics (AFA) technology to improve the performance of microtiter plate enzyme-linked immunosorbent assays (ELISAs). Experiments were performed with commercially available AFA instrumentation and off-the-shelf 96-well microtiter plate sandwich ELISAs. AFA was applied over a range of acoustic energies, temperatures, and durations to the antigen/antibody binding step of an ELISA for measuring HIV-1 p24 in tissue culture samples. AFA-mediated antigen/antibody binding was enhanced up to 2-fold over passive binding at comparable temperatures and was superior or comparable at low temperature (8-10 °C) to passive binding at 37 °C. Lower nonspecific binding (NSB), lower inter- and intra-assay coefficients of variation (CVs), higher Z' factors, and lower limits of detection (LODs) were measured in AFA-mediated assays compared with conventional passive binding. In a more limited study, AFA enhancement of antigen/antibody binding and lower NSB was measured in an ELISA for measuring IGFBP-3 in human plasma. We conclude from this study that application of AFA to antigen/antibody binding steps in microtiter plate ELISAs can enhance key assay performance parameters, particularly Z' factors and LODs. These features render AFA-mediated binding assays potentially more useful in applications such as high-throughput screening and in vitro diagnostics than assays processed with conventional passive antigen/antibody binding steps.

  5. 25 CFR 1000.89 - Can the terms and conditions in an AFA be amended during the year it is in effect?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Can the terms and conditions in an AFA be amended during... Affairs Programs Determining What Programs May Be Included in An Afa § 1000.89 Can the terms and conditions in an AFA be amended during the year it is in effect? Yes, terms and conditions in an AFA may...

  6. 25 CFR 1000.104 - Can funding amounts negotiated in an AFA be adjusted during the year it is in effect?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Can funding amounts negotiated in an AFA be adjusted... Affairs Programs Determining Afa Amounts § 1000.104 Can funding amounts negotiated in an AFA be adjusted during the year it is in effect? Yes, funding amounts negotiated in an AFA may be adjusted under...

  7. 25 CFR 1000.89 - Can the terms and conditions in an AFA be amended during the year it is in effect?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Can the terms and conditions in an AFA be amended during... Affairs Programs Determining What Programs May Be Included in An Afa § 1000.89 Can the terms and conditions in an AFA be amended during the year it is in effect? Yes, terms and conditions in an AFA may...

  8. 25 CFR 1000.104 - Can funding amounts negotiated in an AFA be adjusted during the year it is in effect?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Can funding amounts negotiated in an AFA be adjusted... Affairs Programs Determining Afa Amounts § 1000.104 Can funding amounts negotiated in an AFA be adjusted during the year it is in effect? Yes, funding amounts negotiated in an AFA may be adjusted under...

  9. 25 CFR 1000.89 - Can the terms and conditions in an AFA be amended during the year it is in effect?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Can the terms and conditions in an AFA be amended during... Affairs Programs Determining What Programs May Be Included in An Afa § 1000.89 Can the terms and conditions in an AFA be amended during the year it is in effect? Yes, terms and conditions in an AFA may...

  10. 25 CFR 1000.104 - Can funding amounts negotiated in an AFA be adjusted during the year it is in effect?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Can funding amounts negotiated in an AFA be adjusted... Affairs Programs Determining Afa Amounts § 1000.104 Can funding amounts negotiated in an AFA be adjusted during the year it is in effect? Yes, funding amounts negotiated in an AFA may be adjusted under...

  11. 25 CFR 1000.89 - Can the terms and conditions in an AFA be amended during the year it is in effect?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Can the terms and conditions in an AFA be amended during... Affairs Programs Determining What Programs May Be Included in An Afa § 1000.89 Can the terms and conditions in an AFA be amended during the year it is in effect? Yes, terms and conditions in an AFA may...

  12. 25 CFR 1000.104 - Can funding amounts negotiated in an AFA be adjusted during the year it is in effect?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Can funding amounts negotiated in an AFA be adjusted... Affairs Programs Determining Afa Amounts § 1000.104 Can funding amounts negotiated in an AFA be adjusted during the year it is in effect? Yes, funding amounts negotiated in an AFA may be adjusted under...

  13. 25 CFR 1000.104 - Can funding amounts negotiated in an AFA be adjusted during the year it is in effect?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Can funding amounts negotiated in an AFA be adjusted... Affairs Programs Determining Afa Amounts § 1000.104 Can funding amounts negotiated in an AFA be adjusted during the year it is in effect? Yes, funding amounts negotiated in an AFA may be adjusted under...

  14. 25 CFR 1000.89 - Can the terms and conditions in an AFA be amended during the year it is in effect?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Can the terms and conditions in an AFA be amended during... Affairs Programs Determining What Programs May Be Included in An Afa § 1000.89 Can the terms and conditions in an AFA be amended during the year it is in effect? Yes, terms and conditions in an AFA may...

  15. Combination pipe-rupture mitigator and in-vessel core catcher. [LMFBR

    DOEpatents

    Tilbrook, R.W.; Markowski, F.J.

    1982-03-09

    A device is described which mitigates against the effects of a failed coolant loop in a nuclear reactor by restricting the outflow of coolant from the reactor through the failed loop and by retaining any particulated debris from a molten core which may result from coolant loss or other cause. The device reduces the reverse pressure drop through the failed loop by limiting the access of coolant in the reactor to the inlet of the failed loop. The device also spreads any particulated core debris over a large area to promote cooling.

  16. Differential recognition of members of the carcinoembryonic antigen family by Afa/Dr adhesins of diffusely adhering Escherichia coli (Afa/Dr DAEC).

    PubMed

    Berger, Cedric N; Billker, Oliver; Meyer, Thomas F; Servin, Alain L; Kansau, Imad

    2004-05-01

    Little is known about the molecular bases underlying the virulence of diffusely adhering Escherichia coli (DAEC) harbouring the Afa/Dr family of adhesins. These adhesins recognize as receptors the GPI-anchored proteins CD55 (decay-accelerating factor, DAF) and CD66e (carcinoembryonic antigen, CEA). CD66e is a member of the CEA-related cell adhesion molecules (CEACAM) family, comprising seven members. We analysed the interactions of Afa/Dr DAEC with the CEACAMs using CEACAM-expressing CHO and HeLa cells. The results demonstrate that only E. coli expressing a subfamily of Afa/Dr adhesins, named here Afa/Dr-I, including Dr, F1845 and AfaE-III adhesins, bound onto CHO cells expressing CEACAM1, CEA or CEACAM6. Whereas all the Afa/Dr adhesins elicit recruitment of CD55 around adhering bacteria, only the Afa/Dr-I subfamily elicits the recruitment of CEACAM1, CEA and CEACAM6. In addition, although CEACAM3 is not recognized as a receptor by the subfamily of Afa/Dr adhesins, it is recruited around bacteria in HeLa cells. The recruited CEACAM1, CEA and CEACAM6 around adhering bacteria resist totally or in part a detergent extraction, whereas the recruited CEACAM3 does not. Finally, the results show that recognition of CEA and CEACAM6, but not CEACAM1, is accompanied by tight attachment to bacteria of cell surface microvilli-like extensions, which are elongated. Moreover, recognition of CEA is accompanied by an activation of the Rho GTPase Cdc42 and by a phosphorylation of ERM, which in turn elicit the observed cell surface microvilli-like extensions.

  17. AFA and F17 adhesins produced by pathogenic Escherichia coli strains in domestic animals.

    PubMed

    Le Bouguénec, C; Bertin, Y

    1999-01-01

    AFA and F17 are afimbrial and fimbrial adhesins, respectively, produced by pathogenic Escherichia coli strains in domestic animals. F17-related fimbriae are mainly detected on bovine and ovine E. coli associated with diarrhoea or septicaemia. The F17-G adhesin subunits recognize N-acetyl-D-glucosamine (GlcNAc) receptors present on bovine intestinal cells. Some F17 subtypes also bind to GlcNAc receptors present on human uroepithelial and intestinal Caco-2 cells or to the laminin contained in the basement of mammalian membranes. F17 is often associated with other virulence factors (aerobactin, serum resistance, CNF2 toxin, K99, CS31A or AFA adhesins) on pathogenic E. coli. A cluster of only four genes is required to synthesize functional F17-related fimbrial structures. The hypothesis of multifunctional F17 fimbrial subunits is supported by the fact that: i) the N-terminal part of the adhesin subunit participates in receptor recognition, whereas the C-terminal part is required for biogenesis of the fimbrial filament; and ii) the interaction between structural and adhesin subunits seems to be crucial for the initiation of monomer polymerization. Recently, determinants related to the afa gene clusters from human pathogenic E. coli associated with intestinal and extra-intestinal infections were identified in strains isolated from calves and piglets with diarrhoea and septicaemia. Two afa-related gene clusters, designated afa-7 and afa-8, that encode afimbrial adhesins were cloned and characterized from bovine pathogenic E. coli. These animal afa gene clusters were plasmid and chromosome borne and were expressed by strains that produced other virulence factors such as CNF toxins, F17, PAP and CS31A adhesins. A high frequency of afa-8 and a low prevalence of afa-7 among bovine E. coli isolates were suggested by preliminary epidemiological studies. As with the human afa gene clusters, the animal ones encode an adhesive structure composed of two proteins: AfaE which

  18. Non-Seismology Seismology: Using QuakeCatchers to Analyze the Frequency of Bridge Vibrations

    NASA Astrophysics Data System (ADS)

    Courtier, A. M.; Constantin, C.; Wilson, C. F.

    2013-12-01

    We conducted an experiment to test the feasibility of measuring seismic waves generated by traffic near James Madison University. We used QuakeCatcher seismometers (originally designed for passive seismic measurement) to measure vibrations associated with traffic on a wooden bridge as well as a nearby concrete bridge. This experiment was a signal processing exercise for a student research project and did not draw any conclusions regarding bridge safety or security. The experiment consisted of two temporary measurement stations comprised of a laptop computer and a QuakeCatcher - a small seismometer that plugs directly into the laptop via a USB cable. The QuakeCatcher was taped to the ground at the edge of the bridge to achieve good coupling, and vibrational events were triggered repeatedly with a control vehicle to accumulate a consistent dataset of the bridge response. For the wooden bridge, the resulting 'seismograms' were converted to Seismic Analysis Code (SAC) format and analyzed in MATLAB. The concrete bridge did not generate vibrations significant enough to trigger the recording mechanism on the QuakeCatchers. We will present an overview of the experimental design and frequency content of the traffic patterns, as well as a discussion of the instructional benefits of using the QuakeCatcher sensors in this non-traditional setting.

  19. Large-Scale Testing of Effects of Anti-Foam Agent on Gas Holdup in Process Vessels in the Hanford Waste Treatment Plant

    SciTech Connect

    Mahoney, L.A.; Alzheimer, J.M.; Arm, S.T.; Guzman-Leong, C.E.; Jagoda, L.K.; Stewart, C.W.; Wells, B.E.; Yokuda, S.T.

    2008-07-01

    The Hanford Waste Treatment and Immobilization Plant (WTP) will vitrify the radioactive wastes stored in underground tanks. These wastes generate and retain hydrogen and other flammable gases that create safety concerns for the vitrification process tanks in the WTP. An anti-foam agent (AFA) will be added to the WTP process streams. Previous testing in a bubble column and a small-scale impeller-mixed vessel indicated that gas holdup in a high-level waste chemical simulant with AFA was as much as 10 times higher than in clay simulant without AFA. This raised a concern that major modifications to the WTP design or qualification of an alternative AFA might be required to satisfy plant safety criteria. However, because the mixing and gas generation mechanisms in the small-scale tests differed from those expected in WTP process vessels, additional tests were performed in a large-scale prototypic mixing system with in situ gas generation. This paper presents the results of this test program. The tests were conducted at Pacific Northwest National Laboratory in a 1/4-scale model of the lag storage process vessel using pulse jet mixers and air spargers. Holdup and release of gas bubbles generated by hydrogen peroxide decomposition were evaluated in waste simulants containing an AFA over a range of Bingham yield stresses and gas generation rates. Results from the 1/4-scale test stand showed that, contrary to the small-scale impeller-mixed tests, holdup in the chemical waste simulant with AFA was not so greatly increased compared to gas holdup in clay without AFA. The test stand, simulants, scaling and data-analysis methods, and results are described in relation to previous tests and anticipated WTP operating conditions. (authors)

  20. Large-Scale Testing of Effects of Anti-Foam Agent on Gas Holdup in Process Vessels in the Hanford Waste Treatment Plant - 8280

    SciTech Connect

    Mahoney, Lenna A.; Alzheimer, James M.; Arm, Stuart T.; Guzman-Leong, Consuelo E.; Jagoda, Lynette K.; Stewart, Charles W.; Wells, Beric E.; Yokuda, Satoru T.

    2008-06-03

    The Hanford Waste Treatment Plant (WTP) will vitrify the radioactive wastes stored in underground tanks. These wastes generate and retain hydrogen and other flammable gases that create safety concerns for the vitrification process tanks in the WTP. An anti-foam agent (AFA) will be added to the WTP process streams. Prior testing in a bubble column and a small-scale impeller-mixed vessel indicated that gas holdup in a high-level waste chemical simulant with AFA was up to 10 times that in clay simulant without AFA. This raised a concern that major modifications to the WTP design or qualification of an alternative AFA might be required to satisfy plant safety criteria. However, because the mixing and gas generation mechanisms in the small-scale tests differed from those expected in WTP process vessels, additional tests were performed in a large-scale prototypic mixing system with in situ gas generation. This paper presents the results of this test program. The tests were conducted at Pacific Northwest National Laboratory in a ¼-scale model of the lag storage process vessel using pulse jet mixers and air spargers. Holdup and release of gas bubbles generated by hydrogen peroxide decomposition were evaluated in waste simulants containing an AFA over a range of Bingham yield stresses and gas gen geration rates. Results from the ¼-scale test stand showed that, contrary to the small-scale impeller-mixed tests, gas holdup in clay without AFA is comparable to that in the chemical waste simulant with AFA. The test stand, simulants, scaling and data-analysis methods, and results are described in relation to previous tests and anticipated WTP operating conditions.

  1. 78 FR 23683 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-22

    ... Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Trawl Gear in the Central Regulatory Area... directed fishing for Pacific cod by catcher/processors (C/Ps) using trawl gear in the Central Regulatory... of the 2013 Pacific cod total allowable catch apportioned to C/Ps using trawl gear in the...

  2. 75 FR 8840 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Pot...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-26

    ... Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Pot Gear in the Bering Sea and Aleutian... for Pacific cod by pot catcher/processors in the Bering Sea and Aleutian Islands management area (BSAI). This action is necessary to prevent exceeding the A season apportionment of the 2010 Pacific cod...

  3. 77 FR 54837 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-06

    ... Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Trawl Gear in the Western Regulatory Area... directed fishing for Pacific cod by catcher/processors (C/Ps) using trawl gear in the Western Regulatory Area of the Gulf of Alaska (GOA). This action is necessary to prevent exceeding the 2012 Pacific...

  4. 77 FR 21683 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/processors Using...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-11

    ... Economic Zone Off Alaska; Pacific Cod by Catcher/processors Using Trawl Gear in the Central Regulatory Area... directed fishing for Pacific cod by catcher/processors (C/Ps) using trawl gear in the Central Regulatory... of the 2012 Pacific cod total allowable catch apportioned to C/Ps using trawl gear in the...

  5. 78 FR 7280 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Pot...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-01

    ... Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Pot Gear in the Bering Sea and Aleutian... for Pacific cod by pot catcher/processors in the Bering Sea and Aleutian Islands management area (BSAI). This action is necessary to prevent exceeding the A season apportionment of the 2013 Pacific cod...

  6. 78 FR 4346 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-22

    ... Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Trawl Gear in the Western Regulatory Area... directed fishing for Pacific cod by catcher/processors (C/Ps) using trawl gear in the Western Regulatory... fisheries data in a timely fashion and would delay the directed fishing closure of Pacific cod for...

  7. City of Durham, N.C. Department of Public Works Awarded 2015 EPA Region 4 Rain Catcher Award

    EPA Pesticide Factsheets

    ATLANTA - The Environmental Protection Agency (EPA) recognized the City of Durham Department of Public Works with the regional 2015 EPA Rain Catcher Award in the Municipal Category for the Rain Catchers project in Durham, N.C. The award was given at

  8. 50 CFR 600.1105 - Longline catcher processor subsector of the Bering Sea and Aleutian Islands (BSAI) non-pollock...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... the Bering Sea and Aleutian Islands (BSAI) non-pollock groundfish fishery program. 600.1105 Section... Capacity Reduction Regulations § 600.1105 Longline catcher processor subsector of the Bering Sea and... catcher processor subsector of the Bering Sea and Aleutian Islands (BSAI) non-pollock groundfish...

  9. 50 CFR 600.1105 - Longline catcher processor subsector of the Bering Sea and Aleutian Islands (BSAI) non-pollock...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... the Bering Sea and Aleutian Islands (BSAI) non-pollock groundfish fishery program. 600.1105 Section... Capacity Reduction Regulations § 600.1105 Longline catcher processor subsector of the Bering Sea and... catcher processor subsector of the Bering Sea and Aleutian Islands (BSAI) non-pollock groundfish...

  10. 50 CFR 600.1105 - Longline catcher processor subsector of the Bering Sea and Aleutian Islands (BSAI) non-pollock...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... the Bering Sea and Aleutian Islands (BSAI) non-pollock groundfish fishery program. 600.1105 Section... Capacity Reduction Regulations § 600.1105 Longline catcher processor subsector of the Bering Sea and... catcher processor subsector of the Bering Sea and Aleutian Islands (BSAI) non-pollock groundfish...

  11. 50 CFR 600.1105 - Longline catcher processor subsector of the Bering Sea and Aleutian Islands (BSAI) non-pollock...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... the Bering Sea and Aleutian Islands (BSAI) non-pollock groundfish fishery program. 600.1105 Section... Capacity Reduction Regulations § 600.1105 Longline catcher processor subsector of the Bering Sea and... catcher processor subsector of the Bering Sea and Aleutian Islands (BSAI) non-pollock groundfish...

  12. 76 FR 39794 - Fisheries of the Economic Exclusive Zone Off Alaska; Shallow-Water Species Fishery by Catcher...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-07

    ... Exclusive Zone Off Alaska; Shallow- Water Species Fishery by Catcher/Processors in the Gulf of Alaska AGENCY... the shallow-water species fishery for catcher/processors subject to sideboard limits established under... 2011 Pacific halibut prohibited species catch (PSC) sideboard limit specified for the...

  13. 76 FR 43934 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Ocean Perch for Catcher/Processors...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-22

    ... Economic Zone Off Alaska; Pacific Ocean Perch for Catcher/Processors Participating in the Rockfish Limited...; closure. SUMMARY: NMFS is prohibiting directed fishing for Pacific ocean perch by catcher/processors...). This action is necessary to prevent exceeding the 2011 total allowable catch (TAC) of Pacific...

  14. 75 FR 42337 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Ocean Perch for Catcher/Processors...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-21

    ... Economic Zone Off Alaska; Pacific Ocean Perch for Catcher/Processors Participating in the Rockfish Limited...; closure. SUMMARY: NMFS is prohibiting directed fishing for Pacific ocean perch by catcher/processors...). This action is necessary to prevent exceeding the 2010 total allowable catch (TAC) of Pacific...

  15. 77 FR 9589 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-17

    ... Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Trawl Gear in the Western Regulatory Area... directed fishing for Pacific cod by catcher/processors (C/Ps) using trawl gear in the Western Regulatory... of the 2012 Pacific cod total allowable catch apportioned to C/Ps using trawl gear in the...

  16. 78 FR 54592 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-05

    ... Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Trawl Gear in the Central Regulatory Area... directed fishing for Pacific cod by catcher/processors (C/Ps) using trawl gear in the Central Regulatory... total allowable catch apportioned to C/ Ps using trawl gear in the Central Regulatory Area of the...

  17. 78 FR 23864 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Hook...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-23

    ... Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Hook-and-line Gear in the Western... directed fishing for Pacific cod by catcher/processors (C/Ps) using hook-and-line gear in the Western... allowance of the 2013 Pacific cod total allowable catch apportioned to C/Ps using hook-and-line gear in...

  18. 77 FR 11776 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Hook...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-28

    ... Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Hook-and-Line Gear in the Central... directed fishing for Pacific cod by catcher/processors (C/Ps) using hook-and-line gear in the Central... allowance of the 2012 Pacific cod total allowable catch apportioned to C/Ps using hook-and-line gear in...

  19. 50 CFR Table 47a to Part 679 - Percent of the AFA Catcher/Processor Sector's Pollock Allocation, Numbers of Chinook Salmon Used...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 371 1.65 Arctic Fjord 940866 3396 4.46 293 68 361 1.60 Northern Glacier 663457 661 3.12 205 48 253 1.12 Pacific Glacier 933627 3357 5.06 332 77 409 1.82 Highland Light 577044 3348 5.14 337 79 416...

  20. 50 CFR Table 47a to Part 679 - Percent of the AFA Catcher/Processor Sector's Pollock Allocation, Numbers of Chinook Salmon Used...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 371 1.65 Arctic Fjord 940866 3396 4.46 293 68 361 1.60 Northern Glacier 663457 661 3.12 205 48 253 1.12 Pacific Glacier 933627 3357 5.06 332 77 409 1.82 Highland Light 577044 3348 5.14 337 79 416...

  1. 50 CFR Table 47a to Part 679 - Percent of the AFA Catcher/Processor Sector's Pollock Allocation, Numbers of Chinook Salmon Used...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 371 1.65 Arctic Fjord 940866 3396 4.46 293 68 361 1.60 Northern Glacier 663457 661 3.12 205 48 253 1.12 Pacific Glacier 933627 3357 5.06 332 77 409 1.82 Highland Light 577044 3348 5.14 337 79 416...

  2. 50 CFR Table 47a to Part 679 - Percent of the AFA Catcher/Processor Sector's Pollock Allocation, Numbers of Chinook Salmon Used...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 371 1.65 Arctic Fjord 940866 3396 4.46 293 68 361 1.60 Northern Glacier 663457 661 3.12 205 48 253 1.12 Pacific Glacier 933627 3357 5.06 332 77 409 1.82 Highland Light 577044 3348 5.14 337 79 416...

  3. 50 CFR Table 47a to Part 679 - Percent of the AFA Catcher/Processor Sector's Pollock Allocation, Numbers of Chinook Salmon Used...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 371 1.65 Arctic Fjord 940866 3396 4.46 293 68 361 1.60 Northern Glacier 663457 661 3.12 205 48 253 1.12 Pacific Glacier 933627 3357 5.06 332 77 409 1.82 Highland Light 577044 3348 5.14 337 79 416...

  4. The solution structure of the invasive tip complex from Afa/Dr fibrils.

    PubMed

    Cota, Ernesto; Jones, Celine; Simpson, Peter; Altroff, Harri; Anderson, Kirstine L; du Merle, Laurence; Guignot, Julie; Servin, Alain; Le Bouguénec, Chantal; Mardon, Helen; Matthews, Stephen

    2006-10-01

    Afa/Dr family of adhesins are produced by pathogenic Escherichia coli strains that are especially prevalent in chronic diarrhoeal and recurrent urinary tract infections. Most notably, they are found in up to 50% of cystitis cases in children and 30% of pyelonephritis in pregnant women. Afa/Dr adhesins are capped surface fibrils that mediate recognition of the host and subsequent bacterial internalization. Using the newly solved three-dimensional structure of the minimal invasive complex (AfaDE) combined with biochemical and cellular assays, we reveal the architecture of the fibrillar cap and identify a novel mode of synergistic integrin recognition.

  5. Melt spreading code assessment, modifications, and application to the EPR core catcher design.

    SciTech Connect

    Farmer, M. T .; Nuclear Engineering Division

    2009-03-30

    The Evolutionary Power Reactor (EPR) is under consideration by various utilities in the United States to provide base load electrical production, and as a result the design is undergoing a certification review by the U.S. Nuclear Regulatory Commission (NRC). The severe accident design philosophy for this reactor is based upon the fact that the projected power rating results in a narrow margin for in-vessel melt retention by external cooling of the reactor vessel. As a result, the design addresses ex-vessel core melt stabilization using a mitigation strategy that includes: (1) an external core melt retention system to temporarily hold core melt released from the vessel; (2) a layer of 'sacrificial' material that is admixed with the melt while in the core melt retention system; (3) a melt plug in the lower part of the retention system that, when failed, provides a pathway for the mixture to spread to a large core spreading chamber; and finally, (4) cooling and stabilization of the spread melt by controlled top and bottom flooding. The overall concept is illustrated in Figure 1.1. The melt spreading process relies heavily on inertial flow of a low-viscosity admixed melt to a segmented spreading chamber, and assumes that the melt mass will be distributed to a uniform height in the chamber. The spreading phenomenon thus needs to be modeled properly in order to adequately assess the EPR design. The MELTSPREAD code, developed at Argonne National Laboratory, can model segmented, and both uniform and nonuniform spreading. The NRC is thus utilizing MELTSPREAD to evaluate melt spreading in the EPR design. MELTSPREAD was originally developed to support resolution of the Mark I containment shell vulnerability issue. Following closure of this issue, development of MELTSPREAD ceased in the early 1990's, at which time the melt spreading database upon which the code had been validated was rather limited. In particular, the database that was utilized for initial validation consisted

  6. Detection of pap-, sfa- and afa-specific DNA sequences in Escherichia coli strains isolated from extraintestinal material.

    PubMed

    Bogyiová, E; Kmetová, M; Biros, E; Siegfried, L

    2002-01-01

    P-fimbriae, S-fimbriae and AFA-adhesins are virulence factors responsible for adherence of Escherichia coli strains to extraintestinal host-cell surface. Detection of pap-, sfa- and afa-specific sequences performed by PCR revealed 74% pap+, 65% sfa+, and 8.3% afa+ strains in a group of 84 extraintestinal E. coli isolates. Detection in a group of fecal strains showed 29% pap+, 21% sfa+ and 4% afa+ strains. pap together with sfa were found as the most frequent combination (56%) among extraintestinal isolates probably due to localization of pap- and sfa-operons on a common pathogenicity island. The occurrence of afa-specific sequence among 56 urine strains was 11%, although no afa+ strain was detected among 28 gynecological isolates. No strains with detected adhesin operons were found among twenty (24%) extraintestinal E. coli strains.

  7. Understanding "The Catcher in the Rye": A Student Casebook to Issues, Sources, and Historical Documents.

    ERIC Educational Resources Information Center

    Pinsker, Sanford; Pinsker, Ann

    The social, cultural, and historical documents and commentary in this casebook illuminate the reading of "The Catcher in the Rye," a novel that has become an important rite of passage for many young adults. In addition to a literary analysis, the casebook acquaints students with the larger world in which Holden Caulfield, the…

  8. The Dream Catcher Meditation: a therapeutic technique used with American Indian adolescents.

    PubMed

    Robbins, R

    2001-01-01

    This article describes a short-term treatment insight-oriented model for American Indian adolescents, called Dream Catcher Meditation. It is aimed at helping clients' express unconscious conflicts and to facilitate differentiation and healthy mutuality. Though its duration can vary, twelve sessions are outlined here. Session descriptions include goals and sample questions. Also included are anecdotal material and reflections about cultural relevancy.

  9. Regency Centers in Raleigh, NC Awarded 2015 EPA Region 4 Rain Catcher Award

    EPA Pesticide Factsheets

    ATLANTA - The Environmental Protection Agency (EPA) recognized Regency Centers with the regional 2015 EPA Rain Catcher Award in the Commercial Category for the Market at Colonnade project in Raleigh, N.C. The award was given at a ceremony during the

  10. The Quake-Catcher Network: An Innovative Community-Based Seismic Network

    NASA Astrophysics Data System (ADS)

    Saltzman, J.; Cochran, E. S.; Lawrence, J. F.; Christensen, C. M.

    2009-12-01

    The Quake-Catcher Network (QCN) is a volunteer computing seismic network that engages citizen scientists, teachers, and museums to participate in the detection of earthquakes. In less than two years, the network has grown to over 1000 participants globally and continues to expand. QCN utilizes Micro-Electro-Mechanical System (MEMS) accelerometers, in laptops and external to desktop computers, to detect moderate to large earthquakes. One goal of the network is to involve K-12 classrooms and museums by providing sensors and software to introduce participants to seismology and community-based scientific data collection. The Quake-Catcher Network provides a unique opportunity to engage participants directly in the scientific process, through hands-on activities that link activities and outcomes to their daily lives. Partnerships with teachers and museum staff are critical to growth of the Quake Catcher Network. Each participating institution receives a MEMS accelerometer to connect, via USB, to a computer that can be used for hands-on activities and to record earthquakes through a distributed computing system. We developed interactive software (QCNLive) that allows participants to view sensor readings in real time. Participants can also record earthquakes and download earthquake data that was collected by their sensor or other QCN sensors. The Quake-Catcher Network combines research and outreach to improve seismic networks and increase awareness and participation in science-based research in K-12 schools.

  11. Adolescent Journeys: Finding Female Authority in "The Rain Catchers" and "The House on Mango Street"

    ERIC Educational Resources Information Center

    Dubb, Christina Rose

    2007-01-01

    This article compares the first-person narratives of two adolescent girls in the novels "The Rain Catchers" and "The House on Mango Street". I propose that adolescent girls can use literacy to read the world around them as a text and therefore help them to form their own identities enough to ultimately find authority in telling their own stories.…

  12. SpyTag/SpyCatcher cyclization confers resilience to boiling on a mesophilic enzyme.

    PubMed

    Schoene, Christopher; Fierer, Jacob O; Bennett, S Paul; Howarth, Mark

    2014-06-10

    SpyTag is a peptide that spontaneously forms an amide bond with its protein partner SpyCatcher. SpyTag was fused at the N terminus of β-lactamase and SpyCatcher at the C terminus so that the partners could react to lock together the termini of the enzyme. The wild-type enzyme aggregates above 37 °C, with irreversible loss of activity. Cyclized β-lactamase was soluble even after heating at 100 °C; after cooling, the catalytic activity was restored. SpyTag/SpyCatcher cyclization led to a much larger increase in stability than that achieved through point mutation or alternative approaches to cyclization. Cyclized dihydrofolate reductase was similarly resilient. Analyzing unfolding through calorimetry indicated that cyclization did not increase the unfolding temperature but rather facilitated refolding after thermal stress. SpyTag/SpyCatcher sandwiching represents a simple and efficient route to enzyme cyclization, with potential to greatly enhance the robustness of biocatalysts.

  13. 50 CFR 660.160 - Catcher/processor (C/P) Coop Program.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 9 2010-10-01 2010-10-01 false Catcher/processor (C/P) Coop Program. 660.160 Section 660.160 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND... owner other than the person listed as the limited entry permit owner in the NMFS permit database....

  14. 78 FR 19214 - Fisheries of the Exclusive Economic Zone Off Alaska; Monitoring Requirements for American...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-29

    ... input from owners and operators of American Fisheries Act (AFA) catcher vessels and shoreside processors... inside the codend; (2) the installation of software and communication equipment to enhance observer data... saltwater tanks prior to delivery to a shoreside processor. The intent of this requirement is to reduce...

  15. Wind tunnel data of the analysis of heat pipe and wind catcher technology for the built environment

    PubMed Central

    Calautit, John Kaiser; Chaudhry, Hassam Nasarullah; Hughes, Ben Richard

    2015-01-01

    The data presented in this article were the basis for the study reported in the research articles entitled ‘Climate responsive behaviour heat pipe technology for enhanced passive airside cooling’ by Chaudhry and Hughes [10] which presents the passive airside cooling capability of heat pipes in response to gradually varying external temperatures and related to the research article “CFD and wind tunnel study of the performance of a uni-directional wind catcher with heat transfer devices” by Calautit and Hughes [1] which compares the ventilation performance of a standard roof mounted wind catcher and wind catcher incorporating the heat pipe technology. Here, we detail the wind tunnel test set-up and inflow conditions and the methodologies for the transient heat pipe experiment and analysis of the integration of heat pipes within the control domain of a wind catcher design. PMID:26958604

  16. Wind tunnel data of the analysis of heat pipe and wind catcher technology for the built environment.

    PubMed

    Calautit, John Kaiser; Chaudhry, Hassam Nasarullah; Hughes, Ben Richard

    2015-12-01

    The data presented in this article were the basis for the study reported in the research articles entitled 'Climate responsive behaviour heat pipe technology for enhanced passive airside cooling' by Chaudhry and Hughes [10] which presents the passive airside cooling capability of heat pipes in response to gradually varying external temperatures and related to the research article "CFD and wind tunnel study of the performance of a uni-directional wind catcher with heat transfer devices" by Calautit and Hughes [1] which compares the ventilation performance of a standard roof mounted wind catcher and wind catcher incorporating the heat pipe technology. Here, we detail the wind tunnel test set-up and inflow conditions and the methodologies for the transient heat pipe experiment and analysis of the integration of heat pipes within the control domain of a wind catcher design.

  17. 76 FR 21705 - Fisheries of the Exclusive Economic Zone Off Alaska; Catch Accounting in the Longline Catcher...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-18

    ... Alaska; Catch Accounting in the Longline Catcher/Processor Pacific Cod Fishery AGENCY: National Marine... Islands to support different catch accounting methods for Pacific cod catch. NMFS is considering...

  18. Nucleotide sequence of the afimbrial-adhesin-encoding afa-3 gene cluster and its translocation via flanking IS1 insertion sequences.

    PubMed Central

    Garcia, M I; Labigne, A; Le Bouguenec, C

    1994-01-01

    The afa gene clusters encode afimbrial adhesins (AFAs) that are expressed by uropathogenic and diarrhea-associated Escherichia coli strains. The plasmid-borne afa-3 gene cluster is responsible for the biosynthesis of the AFA-III adhesin that belongs to the Dr family of hemagglutinins. Reported in this work is the nucleotide sequence of the 9.2-kb insert of the recombinant plasmid pILL61, which contains the afa-3 gene cluster cloned from a cystitis-associated E. coli strain (A30). The afa-3 gene cluster was shown to contain six open reading frames, designated afaA to afaF. It was organized in two divergent transcriptional units. Five of the six Afa products showed marked homologies with proteins encoded by previously described adhesion systems that allowed us to attribute to each of them a putative function in the biogenesis of the AFA-III adhesin. AfaE was identified as the structural adhesin product, whereas AfaB and AfaC were recognized as periplasmic chaperone and outer membrane anchor proteins, respectively. The AfaA and AfaF products were shown to be homologous to the PapI-PapB transcriptional regulatory proteins. No function could be attributed to the AfaD product, the gene of which was previously shown to be dispensable for the synthesis of a functional adhesin. Upstream of the afa-3 gene cluster, a 1.2-kb region was found to be 96% identical to the RepFIB sequence of one of the enterotoxigenic E. coli plasmids (P307), suggesting a common ancestor plasmid. This region contains an integrase-like gene (int). Sequence analysis revealed the presence of an IS1 element between the int gene and the afa-3 gene cluster. Two other IS1 elements were detected and located in the vicinity of the afa-3 gene cluster by hybridization experiments. The afa-3 gene cluster was therefore found to be flanked by two IS1 elements in direct orientation and two in opposite orientations. The afa-3 gene cluster, flanked by two directly oriented IS1 elements, was shown to translocate

  19. Nucleotide sequence of the afimbrial-adhesin-encoding afa-3 gene cluster and its translocation via flanking IS1 insertion sequences.

    PubMed

    Garcia, M I; Labigne, A; Le Bouguenec, C

    1994-12-01

    The afa gene clusters encode afimbrial adhesins (AFAs) that are expressed by uropathogenic and diarrhea-associated Escherichia coli strains. The plasmid-borne afa-3 gene cluster is responsible for the biosynthesis of the AFA-III adhesin that belongs to the Dr family of hemagglutinins. Reported in this work is the nucleotide sequence of the 9.2-kb insert of the recombinant plasmid pILL61, which contains the afa-3 gene cluster cloned from a cystitis-associated E. coli strain (A30). The afa-3 gene cluster was shown to contain six open reading frames, designated afaA to afaF. It was organized in two divergent transcriptional units. Five of the six Afa products showed marked homologies with proteins encoded by previously described adhesion systems that allowed us to attribute to each of them a putative function in the biogenesis of the AFA-III adhesin. AfaE was identified as the structural adhesin product, whereas AfaB and AfaC were recognized as periplasmic chaperone and outer membrane anchor proteins, respectively. The AfaA and AfaF products were shown to be homologous to the PapI-PapB transcriptional regulatory proteins. No function could be attributed to the AfaD product, the gene of which was previously shown to be dispensable for the synthesis of a functional adhesin. Upstream of the afa-3 gene cluster, a 1.2-kb region was found to be 96% identical to the RepFIB sequence of one of the enterotoxigenic E. coli plasmids (P307), suggesting a common ancestor plasmid. This region contains an integrase-like gene (int). Sequence analysis revealed the presence of an IS1 element between the int gene and the afa-3 gene cluster. Two other IS1 elements were detected and located in the vicinity of the afa-3 gene cluster by hybridization experiments. The afa-3 gene cluster was therefore found to be flanked by two IS1 elements in direct orientation and two in opposite orientations. The afa-3 gene cluster, flanked by two directly oriented IS1 elements, was shown to translocate

  20. Characterization of plasmid-borne afa-3 gene clusters encoding afimbrial adhesins expressed by Escherichia coli strains associated with intestinal or urinary tract infections.

    PubMed

    Le Bouguenec, C; Garcia, M I; Ouin, V; Desperrier, J M; Gounon, P; Labigne, A

    1993-12-01

    The afa gene clusters encode afimbrial adhesins (AFA) that are expressed by uropathogenic and diarrhea-associated Escherichia coli strains and belong to a family of hemagglutinins recognizing the Dr blood group antigen as a receptor. This family so far includes AFA-I and AFA-III as well as the Dr and F1845 adhesins (B. Nowicki, A. Labigne, S. Moseley, R. Hull, S. Hull, and J. Moulds, Infect. Immun. 58:279-281, 1990). Reported in this work is the genetic organization of the afa-3 gene cluster cloned from a uropathogenic E. coli strain (A30) which expressed a subtype of AFA designated AFA-III. The amino acid sequence of AFA-III was deduced from the nucleotide sequence of the afaE3 gene and was found to be highly homologous to that of the Dr adhesin (98.1% identity). A polymerase chain reaction assay was developed to detect the presence of afa-3 gene clusters in E. coli strains. Study of the genetic support of the afa-3 gene clusters in the strains which showed positive amplification revealed that they were always located on large, 100-kb plasmids whether the strains originated from patients with cystitis or with diarrhea. Moreover, the cloned afa-3 gene clusters from A30 and from the diarrhea-associated strain AL845 appeared to be carried by 9-kb plasmid regions which displayed a similar genetic organization. Chloramphenicol was reported to be a potent inhibitor of receptor binding by the Dr adhesin (Nowicki et al., Infect. Immun. 58:279-281, 1990). AFA-III expressed by strains AL845 and AL847 appeared to mediate, like the Dr adhesin, chloramphenicol-sensitive hemagglutination, whereas AFA-III produced by A30 conferred chloramphenicol-resistant adherence. A comparison of the sequences of these four proteins indicated that the amino acid at position 52 of the processed AFA could be part of the receptor-binding domain.

  1. 75 FR 52308 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-25

    ... Data Report (EDR) for the Catcher/ Processor non-AFA Trawl Sector. OMB Control Number: 0648-0564. Form... Fisheries Act (non-AFA) Trawl Catcher/processor Cooperative Program (Program). The Program established a limited access privilege program for the non-AFA trawl catcher/processor sector. The Amendment 80...

  2. 75 FR 31717 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod for American Fisheries Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-04

    ...: NMFS is prohibiting directed fishing for Pacific cod by American Fisheries Act (AFA) trawl catcher... 2010 Pacific cod directed fishing allowance for AFA trawl catcher processors in the BSAI has been...) allocated to AFA trawl catcher processors in the BSAI is 3,467 metric tons (mt) as established by the...

  3. Structural basis for a hand-like site in the calcium sensor CatchER with fast kinetics

    SciTech Connect

    Zhang, Ying; Reddish, Florence; Tang, Shen; Zhuo, You; Wang, Yuan-Fang; Yang, Jenny J.; Weber, Irene T.

    2013-12-01

    High-resolution crystal structures of the designed calcium sensor CatchER revealed snapshots of calcium and gadolinium ions binding within the designed site in agreement with its fast kinetics. Calcium ions, which are important signaling molecules, can be detected in the endoplasmic reticulum by an engineered mutant of green fluorescent protein (GFP) designated CatchER with a fast off-rate. High resolution (1.78–1.20 Å) crystal structures were analyzed for CatchER in the apo form and in complexes with calcium or gadolinium to probe the binding site for metal ions. While CatchER exhibits a 1:1 binding stoichiometry in solution, two positions were observed for each of the metal ions bound within the hand-like site formed by the carboxylate side chains of the mutated residues S147E, S202D, Q204E, F223E and T225E that may be responsible for its fast kinetic properties. Comparison of the structures of CatchER, wild-type GFP and enhanced GFP confirmed that different conformations of Thr203 and Glu222 are associated with the two forms of Tyr66 of the chromophore which are responsible for the absorbance wavelengths of the different proteins. Calcium binding to CatchER may shift the equilibrium for conformational population of the Glu222 side chain and lead to further changes in its optical properties.

  4. 25 CFR 1000.245 - May a Tribe/Consortium continue work with construction funds remaining in an AFA at the end of...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... funds remaining in an AFA at the end of the funding year? 1000.245 Section 1000.245 Indians OFFICE OF... Construction § 1000.245 May a Tribe/Consortium continue work with construction funds remaining in an AFA at the end of the funding year? Yes, any funds remaining in an AFA at the end of the funding year may...

  5. 25 CFR 1000.98 - How does BIA determine a Tribe's/Consortium's share of funds to be included in an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... funds to be included in an AFA? 1000.98 Section 1000.98 Indians OFFICE OF THE ASSISTANT SECRETARY... Affairs Programs Determining Afa Amounts § 1000.98 How does BIA determine a Tribe's/Consortium's share of funds to be included in an AFA? There are typically two methods for determining the amount of funds...

  6. 25 CFR 1000.465 - May a Tribe/Consortium negotiate AFA provisions on conflicts of interest to take the place of...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false May a Tribe/Consortium negotiate AFA provisions on... § 1000.465 May a Tribe/Consortium negotiate AFA provisions on conflicts of interest to take the place of this subpart? (a) A Tribe/Consortium and the Secretary may agree to AFA provisions, concerning...

  7. 25 CFR 1000.275 - Is it necessary for a self-governance AFA to include any clauses about FTCA coverage?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Is it necessary for a self-governance AFA to include any... for a self-governance AFA to include any clauses about FTCA coverage? No, clauses about FTCA coverage are optional. At the request of Tribes/Consortia, self-governance AFA's shall include the...

  8. 25 CFR 1000.33 - What amount of funding is to be removed from the Consortium's AFA for the withdrawing Tribe?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Consortium's AFA for the withdrawing Tribe? 1000.33 Section 1000.33 Indians OFFICE OF THE ASSISTANT SECRETARY... amount of funding is to be removed from the Consortium's AFA for the withdrawing Tribe? When a Tribe withdraws from a Consortium, the Consortium's AFA must be reduced by the portion of funds attributable...

  9. 25 CFR 1000.33 - What amount of funding is to be removed from the Consortium's AFA for the withdrawing Tribe?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Consortium's AFA for the withdrawing Tribe? 1000.33 Section 1000.33 Indians OFFICE OF THE ASSISTANT SECRETARY... amount of funding is to be removed from the Consortium's AFA for the withdrawing Tribe? When a Tribe withdraws from a Consortium, the Consortium's AFA must be reduced by the portion of funds attributable...

  10. 25 CFR 1000.245 - May a Tribe/Consortium continue work with construction funds remaining in an AFA at the end of...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... funds remaining in an AFA at the end of the funding year? 1000.245 Section 1000.245 Indians OFFICE OF... Construction § 1000.245 May a Tribe/Consortium continue work with construction funds remaining in an AFA at the end of the funding year? Yes, any funds remaining in an AFA at the end of the funding year may...

  11. 25 CFR 1000.409 - Does the Prompt Payment Act (31 U.S.C. 3901) apply to a non-BIA, non-Indian program AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... non-BIA, non-Indian program AFA? 1000.409 Section 1000.409 Indians OFFICE OF THE ASSISTANT SECRETARY... § 1000.409 Does the Prompt Payment Act (31 U.S.C. 3901) apply to a non-BIA, non-Indian program AFA? Yes, upon mutual agreement of the parties, an AFA may incorporate the Prompt Payment Act....

  12. 25 CFR 1000.210 - When does a non-BIA bureau use a public consultation process related to the negotiation of an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... process related to the negotiation of an AFA? 1000.210 Section 1000.210 Indians OFFICE OF THE ASSISTANT... AFA? When required by law or when appropriate under bureau discretion, a bureau may use a public consultation process in negotiating an AFA....

  13. 50 CFR Table 47b to Part 679 - Percent of the AFA Mothership Sector's Pollock Allocation, Numbers of Chinook Salmon Used To...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 11 2011-10-01 2011-10-01 false Percent of the AFA Mothership Sector's... EXCLUSIVE ECONOMIC ZONE OFF ALASKA Pt. 679, Table 47b Table 47b to Part 679—Percent of the AFA Mothership... Documentation No. AFA Permit No. Percent A season B season Annual Percent American Beauty 613847 1688 6.000...

  14. 50 CFR Table 47b to Part 679 - Percent of the AFA Mothership Sector's Pollock Allocation, Numbers of Chinook Salmon Used To...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 13 2013-10-01 2013-10-01 false Percent of the AFA Mothership Sector's... EXCLUSIVE ECONOMIC ZONE OFF ALASKA Pt. 679, Table 47b Table 47b to Part 679—Percent of the AFA Mothership... Documentation No. AFA Permit No. Percent A season B season Annual Percent American Beauty 613847 1688 6.000...

  15. 25 CFR 1000.96 - May a Tribe/Consortium continue to negotiate an AFA pending an appeal of residual functions or...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false May a Tribe/Consortium continue to negotiate an AFA... Agreements for Bureau of Indian Affairs Programs Determining Afa Amounts § 1000.96 May a Tribe/Consortium continue to negotiate an AFA pending an appeal of residual functions or amounts? Yes, pending appeal of...

  16. 25 CFR 1000.98 - How does BIA determine a Tribe's/Consortium's share of funds to be included in an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... funds to be included in an AFA? 1000.98 Section 1000.98 Indians OFFICE OF THE ASSISTANT SECRETARY... Affairs Programs Determining Afa Amounts § 1000.98 How does BIA determine a Tribe's/Consortium's share of funds to be included in an AFA? There are typically two methods for determining the amount of funds...

  17. 25 CFR 1000.461 - What must a Tribe/Consortium do if an organizational conflict of interest arises under an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... conflict of interest arises under an AFA? 1000.461 Section 1000.461 Indians OFFICE OF THE ASSISTANT... What must a Tribe/Consortium do if an organizational conflict of interest arises under an AFA? This section only applies if the conflict was not addressed when the AFA was first negotiated. When a...

  18. 25 CFR 1000.275 - Is it necessary for a self-governance AFA to include any clauses about FTCA coverage?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Is it necessary for a self-governance AFA to include any... for a self-governance AFA to include any clauses about FTCA coverage? No, clauses about FTCA coverage are optional. At the request of Tribes/Consortia, self-governance AFA's shall include the...

  19. 50 CFR Table 47b to Part 679 - Percent of the AFA Mothership Sector's Pollock Allocation, Numbers of Chinook Salmon Used To...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 13 2012-10-01 2012-10-01 false Percent of the AFA Mothership Sector's... EXCLUSIVE ECONOMIC ZONE OFF ALASKA Pt. 679, Table 47b Table 47b to Part 679—Percent of the AFA Mothership... Documentation No. AFA Permit No. Percent A season B season Annual Percent American Beauty 613847 1688 6.000...

  20. 25 CFR 1000.401 - Can a Tribe/Consortium carry over funds not spent during the term of the AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... the term of the AFA? 1000.401 Section 1000.401 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN.../Consortium carry over funds not spent during the term of the AFA? This section applies to BIA programs... under an AFA, if the funds are to be expended in the succeeding fiscal year for the purpose for...

  1. 25 CFR 1000.33 - What amount of funding is to be removed from the Consortium's AFA for the withdrawing Tribe?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Consortium's AFA for the withdrawing Tribe? 1000.33 Section 1000.33 Indians OFFICE OF THE ASSISTANT SECRETARY... amount of funding is to be removed from the Consortium's AFA for the withdrawing Tribe? When a Tribe withdraws from a Consortium, the Consortium's AFA must be reduced by the portion of funds attributable...

  2. 25 CFR 1000.317 - Is a Tribe's/Consortium's general right to negotiate an AFA adversely affected by a reassumption...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... AFA adversely affected by a reassumption action? 1000.317 Section 1000.317 Indians OFFICE OF THE....317 Is a Tribe's/Consortium's general right to negotiate an AFA adversely affected by a reassumption... negotiate an AFA for programs not affected by the reassumption....

  3. 25 CFR 1000.96 - May a Tribe/Consortium continue to negotiate an AFA pending an appeal of residual functions or...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false May a Tribe/Consortium continue to negotiate an AFA... Agreements for Bureau of Indian Affairs Programs Determining Afa Amounts § 1000.96 May a Tribe/Consortium continue to negotiate an AFA pending an appeal of residual functions or amounts? Yes, pending appeal of...

  4. 25 CFR 1000.245 - May a Tribe/Consortium continue work with construction funds remaining in an AFA at the end of...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... funds remaining in an AFA at the end of the funding year? 1000.245 Section 1000.245 Indians OFFICE OF... Construction § 1000.245 May a Tribe/Consortium continue work with construction funds remaining in an AFA at the end of the funding year? Yes, any funds remaining in an AFA at the end of the funding year may...

  5. 25 CFR 1000.402 - After a non-BIA AFA has been executed and the funds transferred to a Tribe/Consortium, can a...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false After a non-BIA AFA has been executed and the funds... Miscellaneous Provisions § 1000.402 After a non-BIA AFA has been executed and the funds transferred to a Tribe... AFA....

  6. 25 CFR 1000.465 - May a Tribe/Consortium negotiate AFA provisions on conflicts of interest to take the place of...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false May a Tribe/Consortium negotiate AFA provisions on... § 1000.465 May a Tribe/Consortium negotiate AFA provisions on conflicts of interest to take the place of this subpart? (a) A Tribe/Consortium and the Secretary may agree to AFA provisions, concerning...

  7. 50 CFR Table 47b to Part 679 - Percent of the AFA Mothership Sector's Pollock Allocation, Numbers of Chinook Salmon Used To...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 9 2010-10-01 2010-10-01 false Percent of the AFA Mothership Sector's... EXCLUSIVE ECONOMIC ZONE OFF ALASKA Pt. 679, Table 47b Table 47b to Part 679—Percent of the AFA Mothership... Documentation No. AFA Permit No. Percent A season B season Annual Percent American Beauty 613847 1688 6.000...

  8. 25 CFR 1000.101 - Are all funds identified as Tribal shares always paid to the Tribe/Consortium under an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... the Tribe/Consortium under an AFA? 1000.101 Section 1000.101 Indians OFFICE OF THE ASSISTANT SECRETARY... Affairs Programs Determining Afa Amounts § 1000.101 Are all funds identified as Tribal shares always paid to the Tribe/Consortium under an AFA? No, at the discretion of the Tribe/Consortium, Tribal...

  9. 25 CFR 1000.84 - Does a Tribe/Consortium have the right to include provisions of Title I of Pub. L. 93-638 in an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... provisions of Title I of Pub. L. 93-638 in an AFA? 1000.84 Section 1000.84 Indians OFFICE OF THE ASSISTANT.../Consortium have the right to include provisions of Title I of Pub. L. 93-638 in an AFA? Yes, under Pub. L... AFA....

  10. 25 CFR 1000.30 - May a Tribe/Consortium be selected to negotiate an AFA under section 403(b)(2) without having or...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false May a Tribe/Consortium be selected to negotiate an AFA under section 403(b)(2) without having or negotiating an AFA under section 403(b)(1)? 1000.30 Section... Applicant Pool § 1000.30 May a Tribe/Consortium be selected to negotiate an AFA under section...

  11. 25 CFR 1000.401 - Can a Tribe/Consortium carry over funds not spent during the term of the AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... the term of the AFA? 1000.401 Section 1000.401 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN.../Consortium carry over funds not spent during the term of the AFA? This section applies to BIA programs... under an AFA, if the funds are to be expended in the succeeding fiscal year for the purpose for...

  12. 25 CFR 1000.461 - What must a Tribe/Consortium do if an organizational conflict of interest arises under an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... conflict of interest arises under an AFA? 1000.461 Section 1000.461 Indians OFFICE OF THE ASSISTANT... What must a Tribe/Consortium do if an organizational conflict of interest arises under an AFA? This section only applies if the conflict was not addressed when the AFA was first negotiated. When a...

  13. 25 CFR 1000.31 - May a Tribe/Consortium be selected to negotiate an AFA under section 403(c) without negotiating...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false May a Tribe/Consortium be selected to negotiate an AFA under section 403(c) without negotiating an AFA under section 403(b)(1) and/or section 403(b)(2)? 1000... Admission into the Applicant Pool § 1000.31 May a Tribe/Consortium be selected to negotiate an AFA...

  14. 25 CFR 1000.317 - Is a Tribe's/Consortium's general right to negotiate an AFA adversely affected by a reassumption...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... AFA adversely affected by a reassumption action? 1000.317 Section 1000.317 Indians OFFICE OF THE....317 Is a Tribe's/Consortium's general right to negotiate an AFA adversely affected by a reassumption... negotiate an AFA for programs not affected by the reassumption....

  15. 25 CFR 1000.402 - After a non-BIA AFA has been executed and the funds transferred to a Tribe/Consortium, can a...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false After a non-BIA AFA has been executed and the funds... Miscellaneous Provisions § 1000.402 After a non-BIA AFA has been executed and the funds transferred to a Tribe... AFA....

  16. 25 CFR 1000.101 - Are all funds identified as Tribal shares always paid to the Tribe/Consortium under an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... the Tribe/Consortium under an AFA? 1000.101 Section 1000.101 Indians OFFICE OF THE ASSISTANT SECRETARY... Affairs Programs Determining Afa Amounts § 1000.101 Are all funds identified as Tribal shares always paid to the Tribe/Consortium under an AFA? No, at the discretion of the Tribe/Consortium, Tribal...

  17. 25 CFR 1000.409 - Does the Prompt Payment Act (31 U.S.C. 3901) apply to a non-BIA, non-Indian program AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... non-BIA, non-Indian program AFA? 1000.409 Section 1000.409 Indians OFFICE OF THE ASSISTANT SECRETARY... § 1000.409 Does the Prompt Payment Act (31 U.S.C. 3901) apply to a non-BIA, non-Indian program AFA? Yes, upon mutual agreement of the parties, an AFA may incorporate the Prompt Payment Act....

  18. 25 CFR 1000.210 - When does a non-BIA bureau use a public consultation process related to the negotiation of an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... process related to the negotiation of an AFA? 1000.210 Section 1000.210 Indians OFFICE OF THE ASSISTANT... AFA? When required by law or when appropriate under bureau discretion, a bureau may use a public consultation process in negotiating an AFA....

  19. 25 CFR 1000.31 - May a Tribe/Consortium be selected to negotiate an AFA under section 403(c) without negotiating...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false May a Tribe/Consortium be selected to negotiate an AFA under section 403(c) without negotiating an AFA under section 403(b)(1) and/or section 403(b)(2)? 1000... Admission into the Applicant Pool § 1000.31 May a Tribe/Consortium be selected to negotiate an AFA...

  20. 25 CFR 1000.33 - What amount of funding is to be removed from the Consortium's AFA for the withdrawing Tribe?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Consortium's AFA for the withdrawing Tribe? 1000.33 Section 1000.33 Indians OFFICE OF THE ASSISTANT SECRETARY... amount of funding is to be removed from the Consortium's AFA for the withdrawing Tribe? When a Tribe withdraws from a Consortium, the Consortium's AFA must be reduced by the portion of funds attributable...

  1. 25 CFR 1000.210 - When does a non-BIA bureau use a public consultation process related to the negotiation of an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... process related to the negotiation of an AFA? 1000.210 Section 1000.210 Indians OFFICE OF THE ASSISTANT... AFA? When required by law or when appropriate under bureau discretion, a bureau may use a public consultation process in negotiating an AFA....

  2. 25 CFR 1000.96 - May a Tribe/Consortium continue to negotiate an AFA pending an appeal of residual functions or...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false May a Tribe/Consortium continue to negotiate an AFA... Agreements for Bureau of Indian Affairs Programs Determining Afa Amounts § 1000.96 May a Tribe/Consortium continue to negotiate an AFA pending an appeal of residual functions or amounts? Yes, pending appeal of...

  3. 25 CFR 1000.461 - What must a Tribe/Consortium do if an organizational conflict of interest arises under an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... conflict of interest arises under an AFA? 1000.461 Section 1000.461 Indians OFFICE OF THE ASSISTANT... What must a Tribe/Consortium do if an organizational conflict of interest arises under an AFA? This section only applies if the conflict was not addressed when the AFA was first negotiated. When a...

  4. 25 CFR 1000.98 - How does BIA determine a Tribe's/Consortium's share of funds to be included in an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... funds to be included in an AFA? 1000.98 Section 1000.98 Indians OFFICE OF THE ASSISTANT SECRETARY... Affairs Programs Determining Afa Amounts § 1000.98 How does BIA determine a Tribe's/Consortium's share of funds to be included in an AFA? There are typically two methods for determining the amount of funds...

  5. 25 CFR 1000.465 - May a Tribe/Consortium negotiate AFA provisions on conflicts of interest to take the place of...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false May a Tribe/Consortium negotiate AFA provisions on... § 1000.465 May a Tribe/Consortium negotiate AFA provisions on conflicts of interest to take the place of this subpart? (a) A Tribe/Consortium and the Secretary may agree to AFA provisions, concerning...

  6. 50 CFR Table 47b to Part 679 - Percent of the AFA Mothership Sector's Pollock Allocation, Numbers of Chinook Salmon Used To...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 13 2014-10-01 2014-10-01 false Percent of the AFA Mothership Sector's... EXCLUSIVE ECONOMIC ZONE OFF ALASKA Pt. 679, Table 47b Table 47b to Part 679—Percent of the AFA Mothership... Documentation No. AFA Permit No. Percent A season B season Annual Percent American Beauty 613847 1688 6.000...

  7. 25 CFR 1000.30 - May a Tribe/Consortium be selected to negotiate an AFA under section 403(b)(2) without having or...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false May a Tribe/Consortium be selected to negotiate an AFA under section 403(b)(2) without having or negotiating an AFA under section 403(b)(1)? 1000.30 Section... Applicant Pool § 1000.30 May a Tribe/Consortium be selected to negotiate an AFA under section...

  8. 25 CFR 1000.33 - What amount of funding is to be removed from the Consortium's AFA for the withdrawing Tribe?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Consortium's AFA for the withdrawing Tribe? 1000.33 Section 1000.33 Indians OFFICE OF THE ASSISTANT SECRETARY... amount of funding is to be removed from the Consortium's AFA for the withdrawing Tribe? When a Tribe withdraws from a Consortium, the Consortium's AFA must be reduced by the portion of funds attributable...

  9. 25 CFR 1000.245 - May a Tribe/Consortium continue work with construction funds remaining in an AFA at the end of...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... funds remaining in an AFA at the end of the funding year? 1000.245 Section 1000.245 Indians OFFICE OF... Construction § 1000.245 May a Tribe/Consortium continue work with construction funds remaining in an AFA at the end of the funding year? Yes, any funds remaining in an AFA at the end of the funding year may...

  10. 25 CFR 1000.402 - After a non-BIA AFA has been executed and the funds transferred to a Tribe/Consortium, can a...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false After a non-BIA AFA has been executed and the funds... Miscellaneous Provisions § 1000.402 After a non-BIA AFA has been executed and the funds transferred to a Tribe... AFA....

  11. 25 CFR 1000.30 - May a Tribe/Consortium be selected to negotiate an AFA under section 403(b)(2) without having or...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false May a Tribe/Consortium be selected to negotiate an AFA under section 403(b)(2) without having or negotiating an AFA under section 403(b)(1)? 1000.30 Section... Applicant Pool § 1000.30 May a Tribe/Consortium be selected to negotiate an AFA under section...

  12. 25 CFR 1000.84 - Does a Tribe/Consortium have the right to include provisions of Title I of Pub. L. 93-638 in an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... provisions of Title I of Pub. L. 93-638 in an AFA? 1000.84 Section 1000.84 Indians OFFICE OF THE ASSISTANT.../Consortium have the right to include provisions of Title I of Pub. L. 93-638 in an AFA? Yes, under Pub. L... AFA....

  13. 25 CFR 1000.275 - Is it necessary for a self-governance AFA to include any clauses about FTCA coverage?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Is it necessary for a self-governance AFA to include any... for a self-governance AFA to include any clauses about FTCA coverage? No, clauses about FTCA coverage are optional. At the request of Tribes/Consortia, self-governance AFA's shall include the...

  14. 25 CFR 1000.98 - How does BIA determine a Tribe's/Consortium's share of funds to be included in an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... funds to be included in an AFA? 1000.98 Section 1000.98 Indians OFFICE OF THE ASSISTANT SECRETARY... Affairs Programs Determining Afa Amounts § 1000.98 How does BIA determine a Tribe's/Consortium's share of funds to be included in an AFA? There are typically two methods for determining the amount of funds...

  15. 25 CFR 1000.30 - May a Tribe/Consortium be selected to negotiate an AFA under section 403(b)(2) without having or...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false May a Tribe/Consortium be selected to negotiate an AFA under section 403(b)(2) without having or negotiating an AFA under section 403(b)(1)? 1000.30 Section... Applicant Pool § 1000.30 May a Tribe/Consortium be selected to negotiate an AFA under section...

  16. 25 CFR 1000.409 - Does the Prompt Payment Act (31 U.S.C. 3901) apply to a non-BIA, non-Indian program AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... non-BIA, non-Indian program AFA? 1000.409 Section 1000.409 Indians OFFICE OF THE ASSISTANT SECRETARY... § 1000.409 Does the Prompt Payment Act (31 U.S.C. 3901) apply to a non-BIA, non-Indian program AFA? Yes, upon mutual agreement of the parties, an AFA may incorporate the Prompt Payment Act....

  17. 25 CFR 1000.317 - Is a Tribe's/Consortium's general right to negotiate an AFA adversely affected by a reassumption...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... AFA adversely affected by a reassumption action? 1000.317 Section 1000.317 Indians OFFICE OF THE....317 Is a Tribe's/Consortium's general right to negotiate an AFA adversely affected by a reassumption... negotiate an AFA for programs not affected by the reassumption....

  18. 25 CFR 1000.317 - Is a Tribe's/Consortium's general right to negotiate an AFA adversely affected by a reassumption...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... AFA adversely affected by a reassumption action? 1000.317 Section 1000.317 Indians OFFICE OF THE....317 Is a Tribe's/Consortium's general right to negotiate an AFA adversely affected by a reassumption... negotiate an AFA for programs not affected by the reassumption....

  19. 25 CFR 1000.96 - May a Tribe/Consortium continue to negotiate an AFA pending an appeal of residual functions or...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false May a Tribe/Consortium continue to negotiate an AFA... Agreements for Bureau of Indian Affairs Programs Determining Afa Amounts § 1000.96 May a Tribe/Consortium continue to negotiate an AFA pending an appeal of residual functions or amounts? Yes, pending appeal of...

  20. 25 CFR 1000.31 - May a Tribe/Consortium be selected to negotiate an AFA under section 403(c) without negotiating...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false May a Tribe/Consortium be selected to negotiate an AFA under section 403(c) without negotiating an AFA under section 403(b)(1) and/or section 403(b)(2)? 1000... Admission into the Applicant Pool § 1000.31 May a Tribe/Consortium be selected to negotiate an AFA...

  1. 25 CFR 1000.409 - Does the Prompt Payment Act (31 U.S.C. 3901) apply to a non-BIA, non-Indian program AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... non-BIA, non-Indian program AFA? 1000.409 Section 1000.409 Indians OFFICE OF THE ASSISTANT SECRETARY... § 1000.409 Does the Prompt Payment Act (31 U.S.C. 3901) apply to a non-BIA, non-Indian program AFA? Yes, upon mutual agreement of the parties, an AFA may incorporate the Prompt Payment Act....

  2. 25 CFR 1000.275 - Is it necessary for a self-governance AFA to include any clauses about FTCA coverage?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Is it necessary for a self-governance AFA to include any... for a self-governance AFA to include any clauses about FTCA coverage? No, clauses about FTCA coverage are optional. At the request of Tribes/Consortia, self-governance AFA's shall include the...

  3. 25 CFR 1000.401 - Can a Tribe/Consortium carry over funds not spent during the term of the AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... the term of the AFA? 1000.401 Section 1000.401 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN.../Consortium carry over funds not spent during the term of the AFA? This section applies to BIA programs... under an AFA, if the funds are to be expended in the succeeding fiscal year for the purpose for...

  4. 25 CFR 1000.401 - Can a Tribe/Consortium carry over funds not spent during the term of the AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... the term of the AFA? 1000.401 Section 1000.401 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN.../Consortium carry over funds not spent during the term of the AFA? This section applies to BIA programs... under an AFA, if the funds are to be expended in the succeeding fiscal year for the purpose for...

  5. 25 CFR 1000.84 - Does a Tribe/Consortium have the right to include provisions of Title I of Pub. L. 93-638 in an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... provisions of Title I of Pub. L. 93-638 in an AFA? 1000.84 Section 1000.84 Indians OFFICE OF THE ASSISTANT.../Consortium have the right to include provisions of Title I of Pub. L. 93-638 in an AFA? Yes, under Pub. L... AFA....

  6. 25 CFR 1000.30 - May a Tribe/Consortium be selected to negotiate an AFA under section 403(b)(2) without having or...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false May a Tribe/Consortium be selected to negotiate an AFA under section 403(b)(2) without having or negotiating an AFA under section 403(b)(1)? 1000.30 Section... Applicant Pool § 1000.30 May a Tribe/Consortium be selected to negotiate an AFA under section...

  7. 25 CFR 1000.401 - Can a Tribe/Consortium carry over funds not spent during the term of the AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... the term of the AFA? 1000.401 Section 1000.401 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN.../Consortium carry over funds not spent during the term of the AFA? This section applies to BIA programs... under an AFA, if the funds are to be expended in the succeeding fiscal year for the purpose for...

  8. 25 CFR 1000.84 - Does a Tribe/Consortium have the right to include provisions of Title I of Pub. L. 93-638 in an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... provisions of Title I of Pub. L. 93-638 in an AFA? 1000.84 Section 1000.84 Indians OFFICE OF THE ASSISTANT.../Consortium have the right to include provisions of Title I of Pub. L. 93-638 in an AFA? Yes, under Pub. L... AFA....

  9. 25 CFR 1000.101 - Are all funds identified as Tribal shares always paid to the Tribe/Consortium under an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... the Tribe/Consortium under an AFA? 1000.101 Section 1000.101 Indians OFFICE OF THE ASSISTANT SECRETARY... Affairs Programs Determining Afa Amounts § 1000.101 Are all funds identified as Tribal shares always paid to the Tribe/Consortium under an AFA? No, at the discretion of the Tribe/Consortium, Tribal...

  10. 25 CFR 1000.210 - When does a non-BIA bureau use a public consultation process related to the negotiation of an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... process related to the negotiation of an AFA? 1000.210 Section 1000.210 Indians OFFICE OF THE ASSISTANT... AFA? When required by law or when appropriate under bureau discretion, a bureau may use a public consultation process in negotiating an AFA....

  11. 25 CFR 1000.409 - Does the Prompt Payment Act (31 U.S.C. 3901) apply to a non-BIA, non-Indian program AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... non-BIA, non-Indian program AFA? 1000.409 Section 1000.409 Indians OFFICE OF THE ASSISTANT SECRETARY... § 1000.409 Does the Prompt Payment Act (31 U.S.C. 3901) apply to a non-BIA, non-Indian program AFA? Yes, upon mutual agreement of the parties, an AFA may incorporate the Prompt Payment Act....

  12. 25 CFR 1000.465 - May a Tribe/Consortium negotiate AFA provisions on conflicts of interest to take the place of...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false May a Tribe/Consortium negotiate AFA provisions on... § 1000.465 May a Tribe/Consortium negotiate AFA provisions on conflicts of interest to take the place of this subpart? (a) A Tribe/Consortium and the Secretary may agree to AFA provisions, concerning...

  13. 25 CFR 1000.465 - May a Tribe/Consortium negotiate AFA provisions on conflicts of interest to take the place of...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false May a Tribe/Consortium negotiate AFA provisions on... § 1000.465 May a Tribe/Consortium negotiate AFA provisions on conflicts of interest to take the place of this subpart? (a) A Tribe/Consortium and the Secretary may agree to AFA provisions, concerning...

  14. 25 CFR 1000.461 - What must a Tribe/Consortium do if an organizational conflict of interest arises under an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... conflict of interest arises under an AFA? 1000.461 Section 1000.461 Indians OFFICE OF THE ASSISTANT... What must a Tribe/Consortium do if an organizational conflict of interest arises under an AFA? This section only applies if the conflict was not addressed when the AFA was first negotiated. When a...

  15. 25 CFR 1000.101 - Are all funds identified as Tribal shares always paid to the Tribe/Consortium under an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... the Tribe/Consortium under an AFA? 1000.101 Section 1000.101 Indians OFFICE OF THE ASSISTANT SECRETARY... Affairs Programs Determining Afa Amounts § 1000.101 Are all funds identified as Tribal shares always paid to the Tribe/Consortium under an AFA? No, at the discretion of the Tribe/Consortium, Tribal...

  16. 25 CFR 1000.31 - May a Tribe/Consortium be selected to negotiate an AFA under section 403(c) without negotiating...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false May a Tribe/Consortium be selected to negotiate an AFA under section 403(c) without negotiating an AFA under section 403(b)(1) and/or section 403(b)(2)? 1000... Admission into the Applicant Pool § 1000.31 May a Tribe/Consortium be selected to negotiate an AFA...

  17. 25 CFR 1000.317 - Is a Tribe's/Consortium's general right to negotiate an AFA adversely affected by a reassumption...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... AFA adversely affected by a reassumption action? 1000.317 Section 1000.317 Indians OFFICE OF THE....317 Is a Tribe's/Consortium's general right to negotiate an AFA adversely affected by a reassumption... negotiate an AFA for programs not affected by the reassumption....

  18. 25 CFR 1000.31 - May a Tribe/Consortium be selected to negotiate an AFA under section 403(c) without negotiating...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false May a Tribe/Consortium be selected to negotiate an AFA under section 403(c) without negotiating an AFA under section 403(b)(1) and/or section 403(b)(2)? 1000... Admission into the Applicant Pool § 1000.31 May a Tribe/Consortium be selected to negotiate an AFA...

  19. 25 CFR 1000.98 - How does BIA determine a Tribe's/Consortium's share of funds to be included in an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... funds to be included in an AFA? 1000.98 Section 1000.98 Indians OFFICE OF THE ASSISTANT SECRETARY... Affairs Programs Determining Afa Amounts § 1000.98 How does BIA determine a Tribe's/Consortium's share of funds to be included in an AFA? There are typically two methods for determining the amount of funds...

  20. 25 CFR 1000.402 - After a non-BIA AFA has been executed and the funds transferred to a Tribe/Consortium, can a...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false After a non-BIA AFA has been executed and the funds... Miscellaneous Provisions § 1000.402 After a non-BIA AFA has been executed and the funds transferred to a Tribe... AFA....

  1. 25 CFR 1000.210 - When does a non-BIA bureau use a public consultation process related to the negotiation of an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... process related to the negotiation of an AFA? 1000.210 Section 1000.210 Indians OFFICE OF THE ASSISTANT... AFA? When required by law or when appropriate under bureau discretion, a bureau may use a public consultation process in negotiating an AFA....

  2. 25 CFR 1000.84 - Does a Tribe/Consortium have the right to include provisions of Title I of Pub. L. 93-638 in an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... provisions of Title I of Pub. L. 93-638 in an AFA? 1000.84 Section 1000.84 Indians OFFICE OF THE ASSISTANT.../Consortium have the right to include provisions of Title I of Pub. L. 93-638 in an AFA? Yes, under Pub. L... AFA....

  3. 25 CFR 1000.461 - What must a Tribe/Consortium do if an organizational conflict of interest arises under an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... conflict of interest arises under an AFA? 1000.461 Section 1000.461 Indians OFFICE OF THE ASSISTANT... What must a Tribe/Consortium do if an organizational conflict of interest arises under an AFA? This section only applies if the conflict was not addressed when the AFA was first negotiated. When a...

  4. 25 CFR 1000.101 - Are all funds identified as Tribal shares always paid to the Tribe/Consortium under an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... the Tribe/Consortium under an AFA? 1000.101 Section 1000.101 Indians OFFICE OF THE ASSISTANT SECRETARY... Affairs Programs Determining Afa Amounts § 1000.101 Are all funds identified as Tribal shares always paid to the Tribe/Consortium under an AFA? No, at the discretion of the Tribe/Consortium, Tribal...

  5. 25 CFR 1000.275 - Is it necessary for a self-governance AFA to include any clauses about FTCA coverage?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Is it necessary for a self-governance AFA to include any... for a self-governance AFA to include any clauses about FTCA coverage? No, clauses about FTCA coverage are optional. At the request of Tribes/Consortia, self-governance AFA's shall include the...

  6. 25 CFR 1000.245 - May a Tribe/Consortium continue work with construction funds remaining in an AFA at the end of...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... funds remaining in an AFA at the end of the funding year? 1000.245 Section 1000.245 Indians OFFICE OF... Construction § 1000.245 May a Tribe/Consortium continue work with construction funds remaining in an AFA at the end of the funding year? Yes, any funds remaining in an AFA at the end of the funding year may...

  7. 25 CFR 1000.402 - After a non-BIA AFA has been executed and the funds transferred to a Tribe/Consortium, can a...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false After a non-BIA AFA has been executed and the funds... Miscellaneous Provisions § 1000.402 After a non-BIA AFA has been executed and the funds transferred to a Tribe... AFA....

  8. 25 CFR 1000.96 - May a Tribe/Consortium continue to negotiate an AFA pending an appeal of residual functions or...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false May a Tribe/Consortium continue to negotiate an AFA... Agreements for Bureau of Indian Affairs Programs Determining Afa Amounts § 1000.96 May a Tribe/Consortium continue to negotiate an AFA pending an appeal of residual functions or amounts? Yes, pending appeal of...

  9. Metalloligands containing aminofulvene-aldiminate (AFA) ligands and their bimetallic complexes.

    PubMed

    Bailey, Philip J; Rahman, Mahmudur; Parsons, Simon; Azhar, Muhammad R; White, Fraser J

    2013-02-28

    A simple and convenient route to η(5)-coordinated Ru and Rh aminofulvene-aldiminate (AFA) complexes is described. The metalloligands [Cp*Ru{η(5)-(Ph(2)AFAH)}][BF(4)] (3), [Cp*Ru{η(5)-(benzyl(2)AFAH)}][OTf] (7), [Cp*Rh{η(5)-(Cy(2)AFA)H}][BF(4)](2) (8) and [Cp*Rh{η(5)-(Cy(2)AFA)}][BF(4)] (9) have been synthesised and characterised. The basicity of 9 has been found to be significantly less than its neutral analogue and thus eliminates the need for a deprotonation step to ligate to a second metal in the κ(2)-N,N'-coordination mode. The reaction of 9 with a palladium precursor provides a mixed-metal complex [Cp*Rh(η(5)/κ(2)-Cy(2)AFA)PdCl(2)][BF(4)] (12). Cyclic voltammetry studies of the Ph(2)AFAH ligand shows an irreversible one electron oxidation peak at +1.0 V (vs. Fc/Fc(+)). Complex 3 shows an irreversible oxidation at +1.5 V and a reduction peak at -1.0 V. The oxidation of 3 occurs on the AFA ligand backbone whereas the structurally analogous neutral 1,2-bis(imidoyl)pentamethyl-ruthenocene shows reversible oxidation at the Ru center.

  10. 25 CFR 1000.246 - Must an AFA that contains a construction project or activity incorporate provisions of Federal...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Must an AFA that contains a construction project or activity incorporate provisions of Federal construction standards? 1000.246 Section 1000.246 Indians OFFICE... Construction § 1000.246 Must an AFA that contains a construction project or activity incorporate provisions...

  11. 25 CFR 1000.164 - Can a Tribe/Consortium have an AFA without entering into a compact?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Can a Tribe/Consortium have an AFA without entering into a compact? 1000.164 Section 1000.164 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... A Self-Governance Compact § 1000.164 Can a Tribe/Consortium have an AFA without entering into...

  12. 25 CFR 1000.129 - Are there any programs that may not be included in an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Are there any programs that may not be included in an AFA? 1000.129 Section 1000.129 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE... § 1000.129 Are there any programs that may not be included in an AFA? Yes, section 403(k) of the...

  13. 25 CFR 1000.164 - Can a Tribe/Consortium have an AFA without entering into a compact?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Can a Tribe/Consortium have an AFA without entering into a compact? 1000.164 Section 1000.164 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... A Self-Governance Compact § 1000.164 Can a Tribe/Consortium have an AFA without entering into...

  14. Representational difference analysis between Afa/Dr diffusely adhering Escherichia coli and nonpathogenic E. coli K-12.

    PubMed

    Blanc-Potard, Anne-Beatrice; Tinsley, Colin; Scaletsky, Isabel; Le Bouguenec, Chantal; Guignot, Julie; Servin, Alain L; Nassif, Xavier; Bernet-Camard, Marie-Francoise

    2002-10-01

    Diffusely adhering Escherichia coli strains harboring Afa/Dr adhesins (Afa/Dr DAEC) have been associated with diarrhea and urinary tract infections (UTIs). The present work is the first extensive molecular study of a Afa/Dr DAEC strain using the representational difference analysis technique. We have searched for DNA sequences present in strain C1845, recovered from a diarrheagenic child, but absent from a nonpathogenic K-12 strain. Strain C1845 harbors part of a pathogenicity island (PAI(CFT073)) and several iron transport systems found in other E. coli pathovars. We did not find genes encoding factors known to subvert host cell proteins, such as type III secretion system or effector proteins. Several C1845-specific sequences are homologous to putative virulence genes or show no homology with known sequences, and we have analyzed their distribution among Afa/Dr and non-Afa/Dr clinical isolates and among strains from the E. coli Reference Collection. Three C1845-specific sequences (MO30, S109, and S111) have a high prevalence (77 to 80%) among Afa/Dr strains and a low prevalence (12 to 23%) among non-Afa/Dr strains. In addition, our results indicate that strain IH11128, an Afa/Dr DAEC strain recovered from a patient with a UTI, is genetically closely related to strain C1845.

  15. 25 CFR 1000.246 - Must an AFA that contains a construction project or activity incorporate provisions of Federal...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Must an AFA that contains a construction project or activity incorporate provisions of Federal construction standards? 1000.246 Section 1000.246 Indians OFFICE... Construction § 1000.246 Must an AFA that contains a construction project or activity incorporate provisions...

  16. 25 CFR 1000.164 - Can a Tribe/Consortium have an AFA without entering into a compact?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Can a Tribe/Consortium have an AFA without entering into a compact? 1000.164 Section 1000.164 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... A Self-Governance Compact § 1000.164 Can a Tribe/Consortium have an AFA without entering into...

  17. 25 CFR 1000.164 - Can a Tribe/Consortium have an AFA without entering into a compact?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Can a Tribe/Consortium have an AFA without entering into a compact? 1000.164 Section 1000.164 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... A Self-Governance Compact § 1000.164 Can a Tribe/Consortium have an AFA without entering into...

  18. 25 CFR 1000.246 - Must an AFA that contains a construction project or activity incorporate provisions of Federal...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Must an AFA that contains a construction project or activity incorporate provisions of Federal construction standards? 1000.246 Section 1000.246 Indians OFFICE... Construction § 1000.246 Must an AFA that contains a construction project or activity incorporate provisions...

  19. 25 CFR 1000.129 - Are there any programs that may not be included in an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Are there any programs that may not be included in an AFA? 1000.129 Section 1000.129 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE... § 1000.129 Are there any programs that may not be included in an AFA? Yes, section 403(k) of the...

  20. 25 CFR 1000.408 - Can a Tribe/Consortium use Federal supply sources in the performance of an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Can a Tribe/Consortium use Federal supply sources in the performance of an AFA? 1000.408 Section 1000.408 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... Tribe/Consortium use Federal supply sources in the performance of an AFA? A Tribe/Consortium and...

  1. 25 CFR 1000.129 - Are there any programs that may not be included in an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Are there any programs that may not be included in an AFA? 1000.129 Section 1000.129 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE... § 1000.129 Are there any programs that may not be included in an AFA? Yes, section 403(k) of the...

  2. 25 CFR 1000.408 - Can a Tribe/Consortium use Federal supply sources in the performance of an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Can a Tribe/Consortium use Federal supply sources in the performance of an AFA? 1000.408 Section 1000.408 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... Tribe/Consortium use Federal supply sources in the performance of an AFA? A Tribe/Consortium and...

  3. 25 CFR 1000.408 - Can a Tribe/Consortium use Federal supply sources in the performance of an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Can a Tribe/Consortium use Federal supply sources in the performance of an AFA? 1000.408 Section 1000.408 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... Tribe/Consortium use Federal supply sources in the performance of an AFA? A Tribe/Consortium and...

  4. 25 CFR 1000.246 - Must an AFA that contains a construction project or activity incorporate provisions of Federal...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Must an AFA that contains a construction project or activity incorporate provisions of Federal construction standards? 1000.246 Section 1000.246 Indians OFFICE... Construction § 1000.246 Must an AFA that contains a construction project or activity incorporate provisions...

  5. 25 CFR 1000.408 - Can a Tribe/Consortium use Federal supply sources in the performance of an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Can a Tribe/Consortium use Federal supply sources in the performance of an AFA? 1000.408 Section 1000.408 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... Tribe/Consortium use Federal supply sources in the performance of an AFA? A Tribe/Consortium and...

  6. 25 CFR 1000.246 - Must an AFA that contains a construction project or activity incorporate provisions of Federal...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Must an AFA that contains a construction project or activity incorporate provisions of Federal construction standards? 1000.246 Section 1000.246 Indians OFFICE... Construction § 1000.246 Must an AFA that contains a construction project or activity incorporate provisions...

  7. 25 CFR 1000.129 - Are there any programs that may not be included in an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Are there any programs that may not be included in an AFA? 1000.129 Section 1000.129 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE... § 1000.129 Are there any programs that may not be included in an AFA? Yes, section 403(k) of the...

  8. 25 CFR 1000.164 - Can a Tribe/Consortium have an AFA without entering into a compact?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Can a Tribe/Consortium have an AFA without entering into a compact? 1000.164 Section 1000.164 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... A Self-Governance Compact § 1000.164 Can a Tribe/Consortium have an AFA without entering into...

  9. 25 CFR 1000.129 - Are there any programs that may not be included in an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Are there any programs that may not be included in an AFA? 1000.129 Section 1000.129 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS, DEPARTMENT OF THE... § 1000.129 Are there any programs that may not be included in an AFA? Yes, section 403(k) of the...

  10. 25 CFR 1000.408 - Can a Tribe/Consortium use Federal supply sources in the performance of an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Can a Tribe/Consortium use Federal supply sources in the performance of an AFA? 1000.408 Section 1000.408 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN AFFAIRS... Tribe/Consortium use Federal supply sources in the performance of an AFA? A Tribe/Consortium and...

  11. 25 CFR 1000.335 - How will retrocession affect the Tribe's/Consortium's existing and future AFAs?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false How will retrocession affect the Tribe's/Consortium's... affect the Tribe's/Consortium's existing and future AFAs? Retrocession does not affect other parts of the AFA or funding agreements with other bureaus. A Tribe/Consortium may request to negotiate for...

  12. 25 CFR 1000.335 - How will retrocession affect the Tribe's/Consortium's existing and future AFAs?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false How will retrocession affect the Tribe's/Consortium's... affect the Tribe's/Consortium's existing and future AFAs? Retrocession does not affect other parts of the AFA or funding agreements with other bureaus. A Tribe/Consortium may request to negotiate for...

  13. 25 CFR 1000.335 - How will retrocession affect the Tribe's/Consortium's existing and future AFAs?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false How will retrocession affect the Tribe's/Consortium's... affect the Tribe's/Consortium's existing and future AFAs? Retrocession does not affect other parts of the AFA or funding agreements with other bureaus. A Tribe/Consortium may request to negotiate for...

  14. 25 CFR 1000.335 - How will retrocession affect the Tribe's/Consortium's existing and future AFAs?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false How will retrocession affect the Tribe's/Consortium's... affect the Tribe's/Consortium's existing and future AFAs? Retrocession does not affect other parts of the AFA or funding agreements with other bureaus. A Tribe/Consortium may request to negotiate for...

  15. 25 CFR 1000.335 - How will retrocession affect the Tribe's/Consortium's existing and future AFAs?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false How will retrocession affect the Tribe's/Consortium's... affect the Tribe's/Consortium's existing and future AFAs? Retrocession does not affect other parts of the AFA or funding agreements with other bureaus. A Tribe/Consortium may request to negotiate for...

  16. 76 FR 76903 - Fisheries of the Exclusive Economic Zone Off Alaska; Reallocation of Pacific Cod in the Bering...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-09

    ... gear sectors to American Fisheries Act (AFA) trawl catcher/ processors and catcher/processors using... apportionment for AFA trawl catcher/processors apportionment and 750 mt to the B season apportionment for catcher/ processors using hook-and-line gear. The C season apportionment of the 2011 Pacific cod...

  17. Concussions experienced by Major League Baseball catchers and umpires: field data and experimental baseball impacts.

    PubMed

    Beyer, Jeffrey A; Rowson, Steven; Duma, Stefan M

    2012-01-01

    Some reports have shown that head injuries in baseball may comprise up to 18.5% of all competitive sports-related head injuries. The objective of this study was to evaluate the response of catcher and umpire masks to impacts at these different regions to discover the impact conditions that represent the greatest risk of injury. A series of 10 events in which a catcher or umpire in Major League Baseball, who experienced a foul ball to the mask that resulted in a concussion, were analyzed through video and data on pitch characteristics. It was found that the impacts were distributed across the face, and the median plate speed was approximately 38 m/s (84 mph). To determine the relative severity of each identified impact location, an instrumented Hybrid III head outfitted with a catcher or umpire mask was impacted with baseballs. Testing at 27 and 38 m/s (60 and 84 mph) suggested that impacts to the center-eyebrow and chin locations were the most severe. Peak linear and rotational accelerations were found to be lower than the suggested injury thresholds. While impacts to a mask result in head accelerations which are near or below levels commonly associated with the lower limits for head injury, the exact injury mechanism is unclear, as concussions are still experienced by the mask wearers.

  18. Sizing of "Mother Ship and Catcher" Concepts for LEO Small Debris Capture

    NASA Technical Reports Server (NTRS)

    Bacon, John B.

    2009-01-01

    Most Low Earth Orbit (LEO) debris lies in a limited number of inclination "bands" associated with launch latitudes, or with specific useful orbit inclinations (such as polar orbits). Such narrow inclination bands generally have a uniform spread over all possible Right Ascensions of Ascending Node (RAANs), creating a different orbit plane for nearly every piece of debris. This complicates concept of rendezvous and capture for debris removal. However, a low-orbiting satellite will always phase in RAAN faster than debris objects in higher orbits at the same inclination, potentially solving the problem. Such a base can serve as a single space-based launch facility (a "mother ship") that can tend and then send tiny individual catcher devices for each debris object, as the facility drifts into the same RAAN as the higher object. This presentation will highlight characteristic system requirements of such an architecture, including structural and navigation requirements, power, mass and dV budgets for both the mother ship and the mass-produced common catcher devices that would clean out selected inclination bands. The altitude and inclination regime over which a band is to be cleared, the size distribution of the debris, and the inclusion of additional mission priorities all affect the sizing of the system. It is demonstrated that major LEO hazardous debris reductions can be realized in each band with a single LEO launch of a single mother ship, with simple attached catchers of total mass less than typical commercial LEO launch capability.

  19. Performance of ion surfing rf-carpets for RI beam gas catcher

    NASA Astrophysics Data System (ADS)

    Arai, Fumiya; Ito, Yuta; Katayama, Ichirou; Schury, Peter; Sonoda, Tetsu; Wada, Michiharu; Wollnik, Hermann

    2014-09-01

    High-energy RI beams produced in-flight by fragmentation or fission are used in ion trap-based precision experiments after being stopped in a gas catcher. The stopped ions can be extracted from the gas catcher as a low energy ion beam. In order to transport and extract ions quickly and efficiently, an rf-carpet (RFC) method utilizing a dc potential gradient has been the standard technique. However, such a method is restricted by the transport time to longer half-life isotopes due to the maximum dc gradient that can be supported before electric discharges occur in the gas catcher. To avoid that limitation, a hybrid technique wherein the dc gradient is replaced by a traveling potential wave was proposed, called ``ion surfing''. Recently, we have demonstrated ion extraction using a circular RFC under low and intermediate pressures. For the first time we demonstrated the ion extraction using an rf-carpet in high-pressure He gas. An efficiency of nearly 100% was obtained at 200 mbar He gas pressure for K+ ions.

  20. Tandem repetitive Afa-family sequences from Leymus racemosus and Psathyrostachys juncea (Poaceae)

    PubMed

    Nagaki; Kishii; Tsujimoto; Sasakuma

    1999-12-01

    Tandem repetitive Afa-family sequences of 340 bp are known to occur in wheat and related species of tribe Triticeae. We isolated six and three Afa-family sequences from Leymus racemosus and Psathyrostachys juncea, respectively, both of which are perennial species. The sequences account for 0.5% and 0.2% of L. racemosus and P. juncea genomes, respectively, and using in situ hybridization were located in subtelomeric and interstitial regions of L. racemosus chromosomes. These sequences are clustered with those of Elymus trachycaulus in the phylogenetic tree. Our findings indicate that the Afa-family sequences have been amplified at least twice in the lineage of L. racemosus, P. juncea, and E. trachycaulus.

  1. [Revised prevalence of afa+ Escherichia coli strains in acute pyelonephritis of children].

    PubMed

    Licznar, P; Eychenne, I; Azéma, C; Decramer, S; Bouissou, F; Fayet, O; Prère, M-F

    2003-10-01

    Two hundred E. coli strains isolated from children with pyelonephritis were investigated for the presence of six virulence factors. The used primers amplified adhesin pap and sfa, toxin haemolysin (hly) and cytotoxic necrotizing factor 1 (cnf1) and aerobactin (aer). For afimbrial adhesin, the previously used set of primers could not allow to detect the newly reported afa operons (Le Bouguenec et al., 2001). With a new set of primers specific for the afa operon family the prevalence of afa+ strains increased from 3.5% to 13.5%. Combinations of three or more factors in a same strain were found in 48.5%. Thirty two different urovirulent genotypes were observed; two strains contained the six studied factors.

  2. 75 FR 9157 - Proposed Information Collection; Comment Request; Alaska Region Scale and Catch Weighing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-01

    ... requirements address performance standards designed to ensure that all catch delivered to the processor is... Community Development Quota Program (CDQ) catcher/processors, American Fisheries Act (AFA) catcher/ processors, AFA motherships, AFA shoreside processors and stationary floating processors, non-AFA...

  3. 50 CFR Table 9 to Part 679 - Groundfish LLP Licenses Eligible for Use in the BSAI Longline Catcher/Processor Subsector, Column...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... in the BSAI Longline Catcher/Processor Subsector, Column A. X Indicates Whether Column B or Column C... the BSAI Longline Catcher/Processor Subsector, Column A. X Indicates Whether Column B or Column C...)(D)(2) LLG 4508 X LLG 1785 X LLG 3681 X LLG 3676 X LLG 3609 X LLG 1400 X LLG 1401 X LLG 3617 X...

  4. The Effect Of Intermittent Vest Cooling On Thermoregulation And Cardiovascular Strain In Baseball Catchers.

    PubMed

    Bishop, Stacy; Szymanski, David J; Ryan, Greg A; Herron, Robert L; Bishop, Phil A

    2017-03-20

    Baseball catchers are exposed to multiple physiological challenges while playing outside during the spring and summer months, many of which deal with recovery and thermoregulation. The purpose of this study was to investigate the effect of intermittent cooling on core temperature, cardiovascular strain, exertion, and recovery during a simulated catching performance in the heat. Six trained college-aged baseball catchers performed in a controlled, hot (35 °C) and humid (25% relative humidity) environment in a counter-balanced, cross-over design. Ice vest cooling (VC) was used as a cooling modality and was compared to a control of no cooling (NC). Rectal temperature (Tre), heart rate (HR), rating of perceived exertion (RPE), and perceived recovery scale (PRS) were recorded before and after each simulated inning. All activity took place in a heat chamber, and each inning consisted of catchers receiving 12 pitches in their position followed by 6 minutes of recovery. Nine total innings were performed, and 27 total innings were performed with each of the two treatments. A significantly smaller mean Tre change was seen in VC when compared to NC (0.58 ± 0.2 °C, 0.98 ± 0.2 °C, p = < 0.01, respectively). RPE was significantly lower and PRS was significantly improved for VC compared to NC (both p ≤ 0.05). Mean recovery HR during VC was significantly lower than NC in the 5th (VC = 84 ± 8 bpm, NC = 90 ± 9 bpm, p = 0.04), 7th (VC = 84 ± 3 bpm, NC = 92 ± 7 bpm, p = 0.02), and 9th (VC = 85 ± 7 bpm, NC = 93 ± 5 bpm, p = 0.01) innings. HR during catching was significantly lower at the end of the VC trials when compared to NC (108 ± 16 bpm versus 120 ± 19 bpm, p = 0.02, respectively). VC decreased heat strain, cardiovascular strain, and RPE while it improved perceived recovery in catchers over a simulated three game series performed in hot conditions.

  5. 25 CFR 1000.146 - Can a Tribe/Consortium negotiate an AFA with a non-BIA bureau for which the performance period...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Can a Tribe/Consortium negotiate an AFA with a non-BIA.../Consortium negotiate an AFA with a non-BIA bureau for which the performance period exceeds one year? Yes, subject to the terms of the AFA, a Tribe/Consortium and a non-BIA bureau may agree to provide for...

  6. 25 CFR 1000.146 - Can a Tribe/Consortium negotiate an AFA with a non-BIA bureau for which the performance period...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Can a Tribe/Consortium negotiate an AFA with a non-BIA.../Consortium negotiate an AFA with a non-BIA bureau for which the performance period exceeds one year? Yes, subject to the terms of the AFA, a Tribe/Consortium and a non-BIA bureau may agree to provide for...

  7. 25 CFR 1000.146 - Can a Tribe/Consortium negotiate an AFA with a non-BIA bureau for which the performance period...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Can a Tribe/Consortium negotiate an AFA with a non-BIA.../Consortium negotiate an AFA with a non-BIA bureau for which the performance period exceeds one year? Yes, subject to the terms of the AFA, a Tribe/Consortium and a non-BIA bureau may agree to provide for...

  8. 25 CFR 1000.147 - Can the terms and conditions in a non-BIA AFA be amended during the year it is in effect?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Can the terms and conditions in a non-BIA AFA be amended during the year it is in effect? 1000.147 Section 1000.147 Indians OFFICE OF THE ASSISTANT SECRETARY...-BIA AFA be amended during the year it is in effect? Yes, terms and conditions in a non-BIA AFA may...

  9. 25 CFR 1000.146 - Can a Tribe/Consortium negotiate an AFA with a non-BIA bureau for which the performance period...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Can a Tribe/Consortium negotiate an AFA with a non-BIA.../Consortium negotiate an AFA with a non-BIA bureau for which the performance period exceeds one year? Yes, subject to the terms of the AFA, a Tribe/Consortium and a non-BIA bureau may agree to provide for...

  10. 25 CFR 1000.146 - Can a Tribe/Consortium negotiate an AFA with a non-BIA bureau for which the performance period...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Can a Tribe/Consortium negotiate an AFA with a non-BIA.../Consortium negotiate an AFA with a non-BIA bureau for which the performance period exceeds one year? Yes, subject to the terms of the AFA, a Tribe/Consortium and a non-BIA bureau may agree to provide for...

  11. 50 CFR Table 47c to Part 679 - Percent of the AFA Inshore Sector's Pollock Allocation, Numbers of Chinook Salmon Used To...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 11 2011-10-01 2011-10-01 false Percent of the AFA Inshore Sector's... EXCLUSIVE ECONOMIC ZONE OFF ALASKA Pt. 679, Table 47c Table 47c to Part 679—Percent of the AFA Inshore.... AFA Permit No. Percent A Season B Season Annual Percent AJ 599164 3405 0.6958 69 41 110 0.31...

  12. 50 CFR Table 47c to Part 679 - Percent of the AFA Inshore Sector's Pollock Allocation, Numbers of Chinook Salmon Used To...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 13 2014-10-01 2014-10-01 false Percent of the AFA Inshore Sector's... EXCLUSIVE ECONOMIC ZONE OFF ALASKA Pt. 679, Table 47c Table 47c to Part 679—Percent of the AFA Inshore.... AFA Permit No. Percent A Season B Season Annual Percent AJ 599164 3405 0.6958 69 41 110 0.31...

  13. 25 CFR 1000.147 - Can the terms and conditions in a non-BIA AFA be amended during the year it is in effect?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Can the terms and conditions in a non-BIA AFA be amended during the year it is in effect? 1000.147 Section 1000.147 Indians OFFICE OF THE ASSISTANT SECRETARY...-BIA AFA be amended during the year it is in effect? Yes, terms and conditions in a non-BIA AFA may...

  14. 50 CFR Table 47c to Part 679 - Percent of the AFA Inshore Sector's Pollock Allocation, Numbers of Chinook Salmon Used To...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 13 2012-10-01 2012-10-01 false Percent of the AFA Inshore Sector's... EXCLUSIVE ECONOMIC ZONE OFF ALASKA Pt. 679, Table 47c Table 47c to Part 679—Percent of the AFA Inshore.... AFA Permit No. Percent A Season B Season Annual Percent AJ 599164 3405 0.6958 69 41 110 0.31...

  15. 25 CFR 1000.147 - Can the terms and conditions in a non-BIA AFA be amended during the year it is in effect?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Can the terms and conditions in a non-BIA AFA be amended during the year it is in effect? 1000.147 Section 1000.147 Indians OFFICE OF THE ASSISTANT SECRETARY...-BIA AFA be amended during the year it is in effect? Yes, terms and conditions in a non-BIA AFA may...

  16. 50 CFR Table 47c to Part 679 - Percent of the AFA Inshore Sector's Pollock Allocation, Numbers of Chinook Salmon Used To...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 13 2013-10-01 2013-10-01 false Percent of the AFA Inshore Sector's... EXCLUSIVE ECONOMIC ZONE OFF ALASKA Pt. 679, Table 47c Table 47c to Part 679—Percent of the AFA Inshore.... AFA Permit No. Percent A Season B Season Annual Percent AJ 599164 3405 0.6958 69 41 110 0.31...

  17. 25 CFR 1000.147 - Can the terms and conditions in a non-BIA AFA be amended during the year it is in effect?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Can the terms and conditions in a non-BIA AFA be amended during the year it is in effect? 1000.147 Section 1000.147 Indians OFFICE OF THE ASSISTANT SECRETARY...-BIA AFA be amended during the year it is in effect? Yes, terms and conditions in a non-BIA AFA may...

  18. 25 CFR 1000.147 - Can the terms and conditions in a non-BIA AFA be amended during the year it is in effect?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Can the terms and conditions in a non-BIA AFA be amended during the year it is in effect? 1000.147 Section 1000.147 Indians OFFICE OF THE ASSISTANT SECRETARY...-BIA AFA be amended during the year it is in effect? Yes, terms and conditions in a non-BIA AFA may...

  19. 76 FR 39790 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Ocean Perch for Catcher Vessels...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-07

    ... to the public interest as it would prevent NMFS from responding to the most recent fisheries data in... National Oceanic and Atmospheric Administration 50 CFR Part 679 RIN 0648-XA543 Fisheries of the Exclusive... Trawl Fishery in the Central Regulatory Area of the Gulf of Alaska AGENCY: National Marine...

  20. 77 FR 13013 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-05

    ... Administrator for Fisheries, NOAA (AA), finds good cause to waive the requirement to provide prior notice and... AA also finds good cause to waive the 30-day delay in the effective date of this action under 5...

  1. 76 FR 17569 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher Vessels Using Trawl...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-30

    ... for Fisheries, NOAA, (AA), finds good cause to waive the requirement to provide prior notice and... recent, relevant data only became available as of March 24, 2011. The AA also finds good cause to...

  2. Plug-and-playable fluorescent cell imaging modular toolkits using the bacterial superglue, SpyTag/SpyCatcher.

    PubMed

    Moon, Hyojin; Bae, Yoonji; Kim, Hansol; Kang, Sebyung

    2016-11-29

    Simple plug-and-playable fluorescent cell imaging modular toolkits are established using the bacterial superglue SpyTag/SpyCatcher protein ligation system. A variety of affibody-fluorescent protein conjugates (AFPCs) are post-translationally generated via the isopeptide bond formation, and each AFPC effectively recognizes and binds to its targeting cells, visualizing them with selective colors on demand.

  3. 50 CFR 600.1108 - Longline catcher processor subsector of the Bering Sea and Aleutian Islands (BSAI) non-pollock...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 12 2014-10-01 2014-10-01 false Longline catcher processor subsector of... 600.1108 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC... each other and the Database, in compliance with the requirements set forth in the Reduction...

  4. 50 CFR 600.1108 - Longline catcher processor subsector of the Bering Sea and Aleutian Islands (BSAI) non-pollock...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 12 2013-10-01 2013-10-01 false Longline catcher processor subsector of... 600.1108 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC... each other and the Database, in compliance with the requirements set forth in the Reduction...

  5. 76 FR 66195 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Pot...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-26

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration 50 CFR Part 679 RIN 0648-XA791 Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Pot Gear in the Bering Sea and...

  6. 75 FR 59157 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Pot...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-27

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration 50 CFR Part 679 RIN 0648-XZ27 Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Pot Gear in the Bering Sea and...

  7. 76 FR 4552 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Pot...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-26

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration 50 CFR Part 679 RIN 0648-XA176 Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Pot Gear in the Bering Sea and...

  8. 77 FR 3638 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Pot...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-25

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration 50 CFR Part 679 RIN 0648-XA955 Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod by Catcher/Processors Using Pot Gear in the Bering Sea and...

  9. catcher: A Software Program to Detect Answer Copying in Multiple-Choice Tests Based on Nominal Response Model

    ERIC Educational Resources Information Center

    Kalender, Ilker

    2012-01-01

    catcher is a software program designed to compute the [omega] index, a common statistical index for the identification of collusions (cheating) among examinees taking an educational or psychological test. It requires (a) responses and (b) ability estimations of individuals, and (c) item parameters to make computations and outputs the results of…

  10. Formation of a nuclear reactor's molten core bath in a crucible-type corium catcher for a nuclear power station equipped with VVER reactors

    NASA Astrophysics Data System (ADS)

    Beshta, S. V.; Vitol', S. A.; Granovskii, V. S.; Kalyago, E. K.; Kovtunova, S. V.; Krushinov, E. V.; Sulatskaya, M. B.; Sulatskii, A. A.; Khabenskii, V. B.; Al'Myashev, V. I.; Gusarov, V. V.

    2011-05-01

    Results from a calculation study on analyzing the formation of a melt bath in a crucible-type catcher for the conditions of a severe accident at a nuclear power station equipped with VVER-1000 reactors are presented. It is shown that the heat loads exerted on the water-cooled walls of the corium catcher shell are limited to a permissible level at which the necessary margins to nucleate boiling crisis and to destruction are ensured under the conditions of thermal and mechanical loading of the shell. An important role of sacrificial material in the efficient operation of the corium catcher is pointed out.

  11. Orientation relationships of Laves phase and NiAl particles in an AFA stainless steel

    NASA Astrophysics Data System (ADS)

    Trotter, Geneva; Baker, Ian

    2015-12-01

    The alumina-forming austenitic (AFA) stainless steel, Fe-20Cr-30Ni-2Nb-5Al (in at. %) was solutionized at 1250 °C in order to obtain a fully austenitic microstructure and then aged for up to 1325 h at 800 °C to precipitate the Laves phase and B2-NiAl particles typically found in AFAs. This paper describes detailed analyses of the orientation relationships between these particles and the matrix which were determined by transmission electron microscopy. Four variants of the (1 1 1)m//(0 0 0 1)p, ?m//? orientation relationship proposed by Denham and Silcock (J. Iron Steel Inst. 207 (1969) p.582) were observed for the Laves phase, and six variants of the (1 1 1)m//(0 1 1)p, ?m//? Kurdjumov-Sachs relationship were observed for the B2-NiAl phase.

  12. Dynamics of tandem repetitive Afa-family sequences in Triticeae, wheat-related species.

    PubMed

    Nagaki, K; Tsujimoto, H; Sasakuma, T

    1998-08-01

    The Afa-family sequences in wheat-related species, Triticeae, are tandem repetitive sequences of 340 bp. All the analyzed Triticeae species carried the sequences in their genomes, though the copy numbers varied about 100-fold among the species. The nucleotide fragments amplified by PCR were cloned and sequenced, and their behavior in the evolution of Triticeae was analyzed by the neighbor-joining (NJ) method. The sequences in genomes with many copies of this family clustered at independent branches of the phylogenic tree, whereas the sequences in genomes with a few copies did not. This may suggest that Afa-family sequences had amplified several times in the evolution of Triticeae, each using a limited number of different master copies. In addition, the sequences of the A and B genomes of hexaploid common wheat indicated that the Afa-family sequences had not evolved in a concerted manner between the genomes. Furthermore, the sequences of each chromosome of the D genome of this species indicated that the sequences had amplified on all over the D-genome chromosomes in a short period.

  13. The Quake-Catcher Network: A Seismic Network for the World

    NASA Astrophysics Data System (ADS)

    Lawrence, J. F.; Cochran, E. S.; Christensen, C. M.; Chung, A. I.

    2012-12-01

    The Quake-Catcher Network (QCN) is a collaborative initiative for developing the world's largest, low-cost strong-motion seismic network by utilizing sensors in and attached to volunteer internet-connected computers. QCN is not only a research tool, but provides an educational tool for teaching earthquake science in formal and informal environments. A central mission of the QCN is to provide scientific educational software and hardware so that K-12 teachers, students, and the general public can better understand and participate in the science of earthquakes and earthquake hazards. The QCN now has over 2000 volunteers worldwide, with concentrations in various earthquake-prone metropolitan areas. The sensors have recorded earthquakes with magnitudes between Ml2.5 (Christchurch, New Zealand - 2010) and Mw8.8 (Maule, Chile - 2010). The peak ground accelerations (PGAs) exhibit the same distribution of scatter as traditional seismic sensors (e.g. the Kinemetrics Epicensor) but with poor resolution at the bottom end. Real-time distributed computing allows for rapid earthquake location and characterization, including magnitude estimation and AlertMap generation. The network is installed and maintained by volunteer seismologists around the world. Because the volunteer provides a free computational platform (a personal computer), internet access, and shelter, the costs of the Quake-Catcher Network are minimal. QCN provides free sensors for individuals and organizations in key regions of interest. QCN provides subsidized sensors (5) for K-12 teachers in the US. QCN provides subsidized sensor at 49 for the general public and $5 for K-12 teachers in the United States. International collaborators are expanding the regions of coverage around the world. With your help, the Quake-Catcher Network can provide better understanding of earthquakes for scientists and the general public. To learn more, visit http://qcn.stanford.edu.arthquake detected near Los Angeles, California on the

  14. Increased rate of apoptosis and diminished phagocytic ability of human neutrophils infected with Afa/Dr diffusely adhering Escherichia coli strains.

    PubMed

    Brest, Patrick; Bétis, Frédéric; Cuburu, Nicolas; Selva, Eric; Herrant, Magali; Servin, Alain; Auberger, Patrick; Hofman, Paul

    2004-10-01

    The proinflammatory effect of Afa/Dr diffusely adhering Escherichia coli (Afa/Dr DAEC) strains have been recently demonstrated in vitro by showing that polymorphonuclear leukocyte (PMN) transepithelial migration is induced after bacterial colonization of apical intestinal monolayers. The effect of Afa/Dr DAEC-PMN interaction on PMN behavior has been not investigated. Because of the putative virulence mechanism of PMN apoptosis during infectious diseases and taking into account the high level of expression of the decay-accelerating factor (DAF, or CD55), the receptor of Afa/Dr DAEC on PMNs, we sought to determine whether infection of PMNs by Afa/Dr DAEC strains could promote cell apoptosis. We looked at the behavior of PMNs incubated with Afa/Dr DAEC strains once they had transmigrated across polarized monolayers of intestinal (T84) cells. Infection of PMNs by Afa/Dr DAEC strains induced PMN apoptosis characterized by morphological nuclear changes, DNA fragmentation, caspase activation, and a high level of annexin V expression. However, transmigrated and nontransmigrated PMNs incubated with Afa/Dr DAEC strains showed similar elevated global caspase activities. PMN apoptosis depended on their agglutination, induced by Afa/Dr DAEC, and was still observed after preincubation of PMNs with anti-CD55 and/or anti-CD66 antibodies. Low levels of phagocytosis of Afa/Dr DAEC strains were observed both in nontransmigrated and in transmigrated PMNs compared to that observed with the control E. coli DH5alpha strain. Taken together, these data strongly suggest that interaction of Afa/Dr DAEC with PMNs may increase the bacterial virulence both by inducing PMN apoptosis through an agglutination process and by diminishing their phagocytic capacity.

  15. 77 FR 34853 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod for American Fisheries Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-12

    ... Economic Zone Off Alaska; Pacific Cod for American Fisheries Act Catcher/Processors Using Trawl Gear in the...: NMFS is prohibiting directed fishing for Pacific cod by American Fisheries Act (AFA) trawl catcher... exceeding the 2012 Pacific cod total allowable catch specified for AFA trawl catcher/processors in the...

  16. Occupational risks of zoonotic infections in Dutch forestry workers and muskrat catchers.

    PubMed

    Moll van Charante, A W; Groen, J; Mulder, P G; Rijpkema, S G; Osterhaus, A D

    1998-02-01

    Lyme borreliosis (LymeB), Hemorrhagic fever with renal syndrome (HemoFRS), Lymphocytic choriomeningitis (LymphoCM), Tick-borne encephalitis (TickBE), Q Fever (QFever), and Weil's leptospirosis (Lepto) are known to be occupational diseases for rural outdoor workers. We investigated the occupational infection risks for these diseases in greater detail. Antibodies to these agents were assessed in 312 forestry workers and muskrat catchers, and in 356 matched office workers. Three levels of occupational exposure were distinguished: high for active forestry workers, low for supervisory forestry staff and muskrat catchers and zero for office workers. At high exposure the prevalence odds ratios (with 95% CI between brackets) were: LymeB 15 (5.5-42), HemoFRS 11 (1.3-501), LymphoCM 5.4 (1.0-50), TickBE 1.0 (0.3-3.0), QFever 1.0 (0.4-2.1), and Lepto 0 (0.0-33). No significant risk of infection was found at low exposure. Part of the present group had also been studied in 1989 and 1990. Within this cohort the conversion rates to Borrelia burgdorferi have been estimated for the periods 1989-1990 and 1990-1993 as 0.23 and 0.066 year(-1), respectively, and the reversion rate as 0.44 year(-1). The corresponding values for HemoFRS were 0.031, 0.018 and 1.42 year(-1). Active forestry workers in the Netherlands are at high risk of infection by LymeB, HemoFRS and LymphoCM. Prevalence of antibodies to LymeB and HemoFRS appeared to reflect the levels of these infection hazards in recent history.

  17. High resolution studies of the Afa/Dr adhesin DraE and its interaction with chloramphenicol.

    PubMed

    Pettigrew, David; Anderson, Kirstine L; Billington, Jason; Cota, Ernesto; Simpson, Peter; Urvil, Petri; Rabuzin, Filip; Roversi, Pietro; Nowicki, Bogdan; du Merle, Laurence; Le Bouguénec, Chantal; Matthews, Stephen; Lea, Susan M

    2004-11-05

    Pathogenic Escherichia coli expressing Afa/Dr adhesins are able to cause both urinary tract and diarrheal infections. The Afa/Dr adhesins confer adherence to epithelial cells via interactions with the human complement regulating protein, decay accelerating factor (DAF or CD55). Two of the Afa/Dr adhesions, AfaE-III and DraE, differ from each other by only three residues but are reported to have several different properties. One such difference is disruption of the interaction between DraE and CD55 by chloramphenicol, whereas binding of AfaE-III to CD55 is unaffected. Here we present a crystal structure of a strand-swapped trimer of wild type DraE. We also present a crystal structure of this trimer in complex with chloramphenicol, as well as NMR data supporting the binding position of chloramphenicol within the crystal. The crystal structure reveals the precise atomic basis for the sensitivity of DraE-CD55 binding to chloramphenicol and demonstrates that in contrast to other chloramphenicol-protein complexes, drug binding is mediated via recognition of the chlorine "tail" rather than via intercalation of the benzene rings into a hydrophobic pocket.

  18. 25 CFR 1000.143 - May the bureaus negotiate terms to be included in an AFA for non-Indian programs?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false May the bureaus negotiate terms to be included in an AFA for non-Indian programs? 1000.143 Section 1000.143 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... an AFA for non-Indian programs? Yes, as provided for by section 403(b)(2) and 403(c) and as...

  19. 25 CFR 1000.123 - Are there non-BIA programs for which the Secretary must negotiate for inclusion in an AFA subject...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Are there non-BIA programs for which the Secretary must negotiate for inclusion in an AFA subject to such terms as the parties may negotiate? 1000.123 Section 1000... there non-BIA programs for which the Secretary must negotiate for inclusion in an AFA subject to...

  20. 25 CFR 1000.123 - Are there non-BIA programs for which the Secretary must negotiate for inclusion in an AFA subject...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Are there non-BIA programs for which the Secretary must negotiate for inclusion in an AFA subject to such terms as the parties may negotiate? 1000.123 Section 1000... there non-BIA programs for which the Secretary must negotiate for inclusion in an AFA subject to...

  1. 25 CFR 1000.301 - When may the Secretary reassume a Federal program operated by a Tribe/Consortium under an AFA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false When may the Secretary reassume a Federal program operated by a Tribe/Consortium under an AFA? 1000.301 Section 1000.301 Indians OFFICE OF THE ASSISTANT... the Secretary reassume a Federal program operated by a Tribe/Consortium under an AFA? The...

  2. 25 CFR 1000.301 - When may the Secretary reassume a Federal program operated by a Tribe/Consortium under an AFA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false When may the Secretary reassume a Federal program operated by a Tribe/Consortium under an AFA? 1000.301 Section 1000.301 Indians OFFICE OF THE ASSISTANT... the Secretary reassume a Federal program operated by a Tribe/Consortium under an AFA? The...

  3. 25 CFR 1000.123 - Are there non-BIA programs for which the Secretary must negotiate for inclusion in an AFA subject...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Are there non-BIA programs for which the Secretary must negotiate for inclusion in an AFA subject to such terms as the parties may negotiate? 1000.123 Section 1000... there non-BIA programs for which the Secretary must negotiate for inclusion in an AFA subject to...

  4. 25 CFR 1000.301 - When may the Secretary reassume a Federal program operated by a Tribe/Consortium under an AFA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false When may the Secretary reassume a Federal program operated by a Tribe/Consortium under an AFA? 1000.301 Section 1000.301 Indians OFFICE OF THE ASSISTANT... the Secretary reassume a Federal program operated by a Tribe/Consortium under an AFA? The...

  5. 25 CFR 1000.143 - May the bureaus negotiate terms to be included in an AFA for non-Indian programs?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false May the bureaus negotiate terms to be included in an AFA for non-Indian programs? 1000.143 Section 1000.143 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... an AFA for non-Indian programs? Yes, as provided for by section 403(b)(2) and 403(c) and as...

  6. 25 CFR 1000.123 - Are there non-BIA programs for which the Secretary must negotiate for inclusion in an AFA subject...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Are there non-BIA programs for which the Secretary must negotiate for inclusion in an AFA subject to such terms as the parties may negotiate? 1000.123 Section 1000... there non-BIA programs for which the Secretary must negotiate for inclusion in an AFA subject to...

  7. 25 CFR 1000.301 - When may the Secretary reassume a Federal program operated by a Tribe/Consortium under an AFA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false When may the Secretary reassume a Federal program operated by a Tribe/Consortium under an AFA? 1000.301 Section 1000.301 Indians OFFICE OF THE ASSISTANT... the Secretary reassume a Federal program operated by a Tribe/Consortium under an AFA? The...

  8. 25 CFR 1000.301 - When may the Secretary reassume a Federal program operated by a Tribe/Consortium under an AFA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false When may the Secretary reassume a Federal program operated by a Tribe/Consortium under an AFA? 1000.301 Section 1000.301 Indians OFFICE OF THE ASSISTANT... the Secretary reassume a Federal program operated by a Tribe/Consortium under an AFA? The...

  9. 25 CFR 1000.143 - May the bureaus negotiate terms to be included in an AFA for non-Indian programs?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false May the bureaus negotiate terms to be included in an AFA for non-Indian programs? 1000.143 Section 1000.143 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... an AFA for non-Indian programs? Yes, as provided for by section 403(b)(2) and 403(c) and as...

  10. 25 CFR 1000.143 - May the bureaus negotiate terms to be included in an AFA for non-Indian programs?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false May the bureaus negotiate terms to be included in an AFA for non-Indian programs? 1000.143 Section 1000.143 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... an AFA for non-Indian programs? Yes, as provided for by section 403(b)(2) and 403(c) and as...

  11. 25 CFR 1000.143 - May the bureaus negotiate terms to be included in an AFA for non-Indian programs?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false May the bureaus negotiate terms to be included in an AFA for non-Indian programs? 1000.143 Section 1000.143 Indians OFFICE OF THE ASSISTANT SECRETARY, INDIAN... an AFA for non-Indian programs? Yes, as provided for by section 403(b)(2) and 403(c) and as...

  12. 25 CFR 1000.123 - Are there non-BIA programs for which the Secretary must negotiate for inclusion in an AFA subject...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Are there non-BIA programs for which the Secretary must negotiate for inclusion in an AFA subject to such terms as the parties may negotiate? 1000.123 Section 1000... there non-BIA programs for which the Secretary must negotiate for inclusion in an AFA subject to...

  13. 50 CFR 679.64 - Harvesting sideboard limits in other fisheries.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Measures § 679.64 Harvesting sideboard limits in other fisheries. (a) Harvesting sideboards for listed AFA catcher/processors. The Regional Administrator will restrict the ability of listed AFA catcher/processors... groundfish fisheries from adverse effects resulting from the AFA and from fishery cooperatives in the...

  14. 50 CFR 679.64 - Harvesting sideboard limits in other fisheries.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 54602, Sept. 12, 2014. (a) Harvesting sideboards for listed AFA catcher/processors. The Regional Administrator will restrict the ability of listed AFA catcher/processors to engage in directed fishing for non... resulting from the AFA and from fishery cooperatives in the BS subarea directed pollock fishery. (1)...

  15. 50 CFR 679.64 - Harvesting sideboard limits in other fisheries.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Measures § 679.64 Harvesting sideboard limits in other fisheries. (a) Harvesting sideboards for listed AFA catcher/processors. The Regional Administrator will restrict the ability of listed AFA catcher/processors... groundfish fisheries from adverse effects resulting from the AFA and from fishery cooperatives in the...

  16. 75 FR 7976 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod for American Fisheries Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-23

    ...: NMFS is prohibiting directed fishing for Pacific cod by American Fisheries Act (AFA) trawl catcher... exceeding the A season allowance of the 2010 Pacific cod total allowable catch (TAC) specified for AFA trawl... cod TAC allocated to AFA trawl catcher-processors in the BSAI is 2,600 metric tons (mt) as...

  17. 77 FR 10668 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Cod for American Fisheries Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-23

    ...: NMFS is prohibiting directed fishing for Pacific cod by American Fisheries Act (AFA) trawl catcher... exceeding the A season allowance of the 2012 Pacific cod total allowable catch (TAC) specified for AFA trawl... cod TAC specified for AFA trawl catcher-processors in the BSAI is 4,021 metric tons as established...

  18. 50 CFR 679.64 - Harvesting sideboard limits in other fisheries.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Measures § 679.64 Harvesting sideboard limits in other fisheries. (a) Harvesting sideboards for listed AFA catcher/processors. The Regional Administrator will restrict the ability of listed AFA catcher/processors... groundfish fisheries from adverse effects resulting from the AFA and from fishery cooperatives in the...

  19. afa-8 Gene cluster is carried by a pathogenicity island inserted into the tRNA(Phe) of human and bovine pathogenic Escherichia coli isolates.

    PubMed

    Lalioui, L; Le Bouguénec, C

    2001-02-01

    We recently described a new afimbrial adhesin, AfaE-VIII, produced by animal strains associated with diarrhea and septicemia and by human isolates associated with extraintestinal infections. Here, we report that the afa-8 operon, encoding AfaE-VIII adhesin, from the human blood isolate Escherichia coli AL862 is carried by a 61-kb genomic region with characteristics typical of a pathogenicity island (PAI), including a size larger than 10 kb, the presence of an integrase-encoding gene, the insertion into a tRNA locus (pheR), and the presence of a small direct repeat at each extremity. Moreover, the G+C content of the afa-8 operon (46.4%) is lower than that of the E. coli K-12/MG1655 chromosome (50.8%). Within this PAI, designated PAI I(AL862), we identified open reading frames able to code for products similar to proteins involved in sugar utilization. Four probes spanning these sequences hybridized with 74.3% of pathogenic afa-8-positive E. coli strains isolated from humans and animals, 25% of human pathogenic afa-8-negative E. coli strains, and only 8% of fecal strains (P = 0.05), indicating that these sequences are strongly associated with the afa-8 operon and that this genetic association may define a PAI widely distributed among human and animal afa-8-positive strains. One of the distinctive features of this study is that E. coli AL862 also carries another afa-8-containing PAI (PAI II(AL862)), which appeared to be similar in size and genetic organization to PAI I(AL862) and was inserted into the pheV gene. We investigated the insertion sites of afa-8-containing PAI in human and bovine pathogenic E. coli strains and found that this PAI preferentially inserted into the pheV gene.

  20. Research on heat transfer characteristics and cold trap capacity of a water catcher during vacuum pre-cooling

    NASA Astrophysics Data System (ADS)

    Liu, Enhai; Liu, Yin; Wang, Changzhong; Liu, Shiqi

    2017-01-01

    Effect of vacuum pre-cooling process on apples was a complex process of heat and mass transfers. The research is based on the physical properties of apples and their heat and mass transfer mechanisms during vacuum pre-cooling. As for the heat transfer characteristics of a water catcher in vacuum pre-cooling, the research studied the heat transfer mechanism and calculated the cold trap capacity by experimental means, and it cold trap capacity were evaluated to supply references for future research into the practical applications of such vacuum pre-cooling techniques. The results provide a theoretical basis for exploring better pre-cooling process conditions and the design of water catchers. The experimental results show that, when the wall temperature of the water catcher is -5°C, the optimal cold trap capacity is about 90.72g and the required cooling capacity is 210.13W in the vacuum pre-cooling of 201.9g of apples.