Sample records for affect nutrient delivery

  1. Factors affecting plant growth in membrane nutrient delivery

    NASA Technical Reports Server (NTRS)

    Dreschel, T. W.; Wheeler, R. M.; Sager, J. C.; Knott, W. M.

    1990-01-01

    The development of the tubular membrane plant growth unit for the delivery of water and nutrients to roots in microgravity has recently focused on measuring the effects of changes in physical variables controlling solution availability to the plants. Significant effects of membrane pore size and the negative pressure used to contain the solution were demonstrated. Generally, wheat grew better in units with a larger pore size but equal negative pressure and in units with the same pore size but less negative pressure. Lettuce also exhibited better plant growth at less negative pressure.

  2. Nutrient and carbon retention in riparian and hillslope forests after mountain pine beetle infestation and timber harvesting in Colorado (Abstract)

    Treesearch

    Chuck Rhoades; Carl Chambers; Kelly Elder; Derek Pierson; Banning Starr

    2012-01-01

    Mountain pine beetle outbreaks have caused an unprecedented amount of tree mortality in northern Colorado and southern Wyoming forests over the past decade. The extensive overstory mortality and associated salvage logging activities may threaten the sustained delivery of clean water from beetle-affected forests. In this study, we evaluate nutrient retention by riparian...

  3. Climate Variability Impacts on Watershed Nutrient Delivery and Reservoir Production

    NASA Astrophysics Data System (ADS)

    White, J. D.; Prochnow, S. J.; Zygo, L. M.; Byars, B. W.

    2005-05-01

    Reservoirs in agricultural dominated watersheds tend to exhibit pulse-system behavior especially if located in climates dominated by summer convective precipitation inputs. Concentration and bulk mass of nutrient and sediment inputs into reservoir systems vary in terms of timing and magnitude of delivery from watershed sources to reservoirs under these climate conditions. Reservoir management often focuses on long-term average inputs without considering short and long-term impacts of variation in loading. In this study we modeled a watershed-reservoir system to assess how climate variability affects reservoir primary production through shifts in external loading and internal recycling of limiting nutrients. The Bosque watershed encompasses 423,824 ha in central Texas which delivers water to Lake Waco, a 2900 ha reservoir that is the primary water source for the city of Waco and surrounding areas. Utilizing the Soil Water Assessment Tool for the watershed and river simulations and the CE-Qual-2e model for the reservoir, hydrologic and nutrient dynamics were simulated for a 10 year period encompassing two ENSO cycles. The models were calibrated based on point measurement of water quality attributes for a two year time period. Results indicated that watershed delivery of nutrients was affected by the presence and density of small flood-control structure in the watershed. However, considerable nitrogen and phosphorus loadings were derived from soils in the upper watershed which have had long-term waste-application from concentrated animal feeding operations. During El Niño years, nutrient and sediment loads increased by 3 times above non-El Niño years. The simulated response within the reservoir to these nutrient and sediment loads had both direct and indirect. Productivity evaluated from chlorophyll a and algal biomass increased under El Niño conditions, however species composition shifts were found with an increase in cyanobacteria dominance. In non-El Niño years, species composition was more evenly distributed. At the longer time scale, El Niño events with accompanying increase in nutrient loads were followed by years in which productivity declined below levels predicted solely by nutrient ratios. This was due to subtle shifts in organic matter decomposition where productive years are followed by increases in refractory material which sequesters nutrients and reduces internal loading.

  4. Microbial biofilm formation and its consequences for the CELSS program

    NASA Technical Reports Server (NTRS)

    Mitchell, R.

    1994-01-01

    A major goal of the Controlled Ecology Life Support System (CELSS) program is to provide reliable and efficient life support systems for long-duration space flights. A principal focus of the program is on the growth of higher plants in growth chambers. These crops should be grown without the risk of damage from microbial contamination. While it is unlikely that plant pathogens will pose a risk, there are serious hazards associated with microorganisms carried in the nutrient delivery systems and in the atmosphere of the growth chamber. Our experience in surface microbiology showed that colonization of surfaces with microorganisms is extremely rapid even when the inoculum is small. After initial colonization extensive biofilms accumulate on moist surfaces. These microbial films metabolize actively and slough off continuously to the air and water. During plant growth in the CELSS program, microbial biofilms have the potential to foul sensors and to plug nutrient delivery systems. In addition both metabolic products of microbial growth and degradation products of materials being considered for use as nutrient reservoirs and for delivery are likely sources of chemicals known to adversly affect plant growth.

  5. Variation in nutrients formulated and nutrients supplied on 5 California dairies.

    PubMed

    Rossow, H A; Aly, S S

    2013-01-01

    Computer models used in ration formulation assume that nutrients supplied by a ration formulation are the same as the nutrients presented in front of the cow in the final ration. Deviations in nutrients due to feed management effects such as dry matter changes (i.e., rain), loading, mixing, and delivery errors are assumed to not affect delivery of nutrients to the cow and her resulting milk production. To estimate how feed management affects nutrients supplied to the cow and milk production, and determine if nutrients can serve as indexes of feed management practices, weekly total mixed ration samples were collected and analyzed for 4 pens (close-up cows, fresh cows, high-milk-producing, and low-milk-producing cows, if available) for 7 to 12 wk on 5 commercial California dairies. Differences among nutrient analyses from these samples and nutrients from the formulated rations were analyzed by PROC MIXED of SAS (SAS Institute Inc., Cary, NC). Milk fat and milk protein percentages did not vary as much [coefficient of variation (CV) = 18 to 33%] as milk yield (kg; CV = 16 to 47 %) across all dairies and pens. Variability in nutrients delivered were highest for macronutrient fat (CV = 22%), lignin (CV = 15%), and ash (CV = 11%) percentages and micronutrients Fe (mg/kg; CV = 48%), Na (%; CV = 42%), and Zn (mg/kg; CV = 38%) for the milking pens across all dairies. Partitioning of the variability in random effects of nutrients delivered and intraclass correlation coefficients showed that variability in lignin percentage of TMR had the highest correlation with variability in milk yield and milk fat percentage, followed by fat and crude protein percentages. But, variability in ash, fat, and lignin percentages of total mixed ration had the highest correlation with variability in milk protein percentage. Therefore, lignin, fat, and ash may be the best indices of feed management to include effects of variability in nutrients on variability in milk yield, milk fat, and milk protein percentages in ration formulation models. Copyright © 2013 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  6. Cross-system nutrient transport: effects of locally-derived aeolian dust on oligotrophic lakes in West Greenland

    NASA Astrophysics Data System (ADS)

    Bullard, J. E.; Anderson, N. J.; McGowan, S.; Prater, C.; Watts, M.; Whitford, E.

    2017-12-01

    Terrestrially-derived nutrients can strongly affect production in aquatic environments. However, while some research has focused on nutrient delivery via hydrological inputs, the effects of atmospheric dry deposition are comparatively understudied. This paper examines the influence of aeolian-derived elements on water chemistry and microbial nutrient-limitation in oligotrophic lakes in West Greenland. Estimates of seasonal dust deposition and elemental leaching rates are combined with lake nutrient concentration measurements to establish the role of glacio-fluvial dust deposition in shaping nutrient stoichiometry of downwind lakes. The bioavailability of dust-associated elements is also explored using enzyme assays designed to indicate nutrient-limitation in microbial communities sampled across a dust deposition gradient. Together, these analyses demonstrate the importance of atmospheric dust inputs on hydrologically-isolated lakes found in arid high-latitude environments and demonstrate the need to better understand the role of aeolian deposition in cross-system nutrient transport.

  7. Biogeochemical processes underpin ecosystem services

    USDA-ARS?s Scientific Manuscript database

    Elemental cycling is critical to the function of ecosystems and delivery of key ecosystem services because many of these elements are essential nutrients or detrimental toxicants that directly affect the health of organisms and ecosystems. A team of authors from North Carolina State University and ...

  8. Efficacy of pectin solution for preventing gastro-esophageal reflux events in patients with percutaneous endoscopic gastrostomy.

    PubMed

    Adachi, Kyoichi; Furuta, Kenji; Aimi, Masahito; Fukazawa, Kousuke; Shimura, Shino; Ohara, Shunji; Nakata, Shuji; Inoue, Yukiko; Ryuko, Kanji; Ishine, Junichi; Katoh, Kyoko; Hirata, Toshiaki; Ohhata, Shuzo; Katoh, Setsushi; Moriyama, Mika; Sumikawa, Masuko; Sanpei, Mari; Kinoshita, Yoshikazu

    2012-05-01

    The aim of this study was to determine the efficacy of pectin solution, which increases the viscosity of liquid nutrient, for prevention of gastro-esophageal reflux in comparison with half-solid nutrient. The subjects were 10 elderly patients undergoing percutaneous endoscopic gastrostomy feeding. Twenty-four-hour esophageal multichannel intraluminal impedance and pH testing was performed during intake of half-solid nutrient and a combination of pectin solution and liquid nutrient. During 4 h after delivery, there was no significant difference in the total number of gastro-esophageal reflux events between the feeding of the half-solid nutrient and the combination of pectin solution and liquid nutrient (5.7 ± 1.2 vs 5.3 ± 1.0/4 h). Acidic reflux after delivery of the half-solid nutrient was significantly more frequent than that after delivery of the combination of pectin solution and liquid nutrient (80.7% vs 60.4%, p = 0.018). The incidence of gastro-esophageal reflux reaching the upper portion of the esophagus tended to be higher during delivery of the half-solid nutrient than during delivery of the combination of pectin solution and liquid nutrient (47.4% vs 34.0%, p = 0.153). In conclusion, the usage of pectin solution combined with liquid nutrient is effective for preventing acidic gastro-esophageal reflux and gastro-esophageal reflux reaching the upper portion of the esophagus.

  9. HYDRAULIC FRACTURING TO IMPROVE NUTRIENT AND OXYGEN DELIVERY FOR IN SITU BIORECLAMATION

    EPA Science Inventory

    The in situ delivery of nutrients and oxygen in soil is a serious problem in implementing in situ biodegradation. Current technology requires ideal site conditions to provide the remediating organisms with the nutrients and oxygen required for their metabolism, but...

  10. Surface-water nutrient conditions and sources in the United States Pacific Northwest

    USGS Publications Warehouse

    Wise, D.R.; Johnson, H.M.

    2011-01-01

    The SPAtially Referenced Regressions On Watershed attributes (SPARROW) model was used to perform an assessment of surface-water nutrient conditions and to identify important nutrient sources in watersheds of the Pacific Northwest region of the United States (U.S.) for the year 2002. Our models included variables representing nutrient sources as well as landscape characteristics that affect nutrient delivery to streams. Annual nutrient yields were higher in watersheds on the wetter, west side of the Cascade Range compared to watersheds on the drier, east side. High nutrient enrichment (relative to the U.S. Environmental Protection Agency's recommended nutrient criteria) was estimated in watersheds throughout the region. Forest land was generally the largest source of total nitrogen stream load and geologic material was generally the largest source of total phosphorus stream load generated within the 12,039 modeled watersheds. These results reflected the prevalence of these two natural sources and the low input from other nutrient sources across the region. However, the combined input from agriculture, point sources, and developed land, rather than natural nutrient sources, was responsible for most of the nutrient load discharged from many of the largest watersheds. Our results provided an understanding of the regional patterns in surface-water nutrient conditions and should be useful to environmental managers in future water-quality planning efforts.

  11. Maternal Dietary Nutrient Intake and Its Association  with Preterm Birth: A Case-control Study in Beijing,  China.

    PubMed

    Zhang, Yan; Zhou, Hong; Perkins, Anthony; Wang, Yan; Sun, Jing

    2017-03-01

    This study aimed to evaluate dietary nutrient intake among Chinese pregnant women by comparison with Chinese Dietary Reference Intakes (DRIs) and to explore the association between dietary nutrients and preterm birth. A case-control design was conducted in Beijing with 130 preterm delivery mothers in case group and 381 term delivery mothers in control group. Information on mothers' diet was collected using a food frequency questionnaire, and nutrients and energy intakes were subsequently calculated based on DRIs. Multivariate analysis of variance was used to compare the differences between term and preterm groups in relation to dietary nutrients. Dietary nutrient intakes were imbalanced in both groups compared with Chinese DRIs. Preterm delivery mothers had a lower level of fat and vitamin E intake than term delivery mothers (p < 0.05). Multivariate analysis showed lower vitamin E intake in preterm delivery mothers with a prepregnancy BMI < 18.5 kg/m2 (p < 0.05) and higher carbohydrate intake in preterm delivery mothers with prepregnancy BMI ≥ 24 kg/m2 (p < 0.05). An imbalanced diet in both groups and low level of dietary intakes of fat and vitamin E in preterm group suggest health education measures should be taken to improve the dietary quality of pregnant women, especially for those with an abnormal prepregnancy BMI.

  12. Surface-Water Nutrient Conditions and Sources in the United States Pacific Northwest1

    PubMed Central

    Wise, Daniel R; Johnson, Henry M

    2011-01-01

    Abstract The SPAtially Referenced Regressions On Watershed attributes (SPARROW) model was used to perform an assessment of surface-water nutrient conditions and to identify important nutrient sources in watersheds of the Pacific Northwest region of the United States (U.S.) for the year 2002. Our models included variables representing nutrient sources as well as landscape characteristics that affect nutrient delivery to streams. Annual nutrient yields were higher in watersheds on the wetter, west side of the Cascade Range compared to watersheds on the drier, east side. High nutrient enrichment (relative to the U.S. Environmental Protection Agency's recommended nutrient criteria) was estimated in watersheds throughout the region. Forest land was generally the largest source of total nitrogen stream load and geologic material was generally the largest source of total phosphorus stream load generated within the 12,039 modeled watersheds. These results reflected the prevalence of these two natural sources and the low input from other nutrient sources across the region. However, the combined input from agriculture, point sources, and developed land, rather than natural nutrient sources, was responsible for most of the nutrient load discharged from many of the largest watersheds. Our results provided an understanding of the regional patterns in surface-water nutrient conditions and should be useful to environmental managers in future water-quality planning efforts. PMID:22457584

  13. Analytic Models of Oxygen and Nutrient Diffusion, Metabolism Dynamics, and Architecture Optimization in Three-Dimensional Tissue Constructs with Applications and Insights in Cerebral Organoids

    PubMed Central

    2016-01-01

    Diffusion models are important in tissue engineering as they enable an understanding of gas, nutrient, and signaling molecule delivery to cells in cell cultures and tissue constructs. As three-dimensional (3D) tissue constructs become larger, more intricate, and more clinically applicable, it will be essential to understand internal dynamics and signaling molecule concentrations throughout the tissue and whether cells are receiving appropriate nutrient delivery. Diffusion characteristics present a significant limitation in many engineered tissues, particularly for avascular tissues and for cells whose viability, differentiation, or function are affected by concentrations of oxygen and nutrients. This article seeks to provide novel analytic solutions for certain cases of steady-state and nonsteady-state diffusion and metabolism in basic 3D construct designs (planar, cylindrical, and spherical forms), solutions that would otherwise require mathematical approximations achieved through numerical methods. This model is applied to cerebral organoids, where it is shown that limitations in diffusion and organoid size can be partially overcome by localizing metabolically active cells to an outer layer in a sphere, a regionalization process that is known to occur through neuroglial precursor migration both in organoids and in early brain development. The given prototypical solutions include a review of metabolic information for many cell types and can be broadly applied to many forms of tissue constructs. This work enables researchers to model oxygen and nutrient delivery to cells, predict cell viability, study dynamics of mass transport in 3D tissue constructs, design constructs with improved diffusion capabilities, and accurately control molecular concentrations in tissue constructs that may be used in studying models of development and disease or for conditioning cells to enhance survival after insults like ischemia or implantation into the body, thereby providing a framework for better understanding and exploring the characteristics and behaviors of engineered tissue constructs. PMID:26650970

  14. Forms and subannual variability of nitrogen and phosphorus loading to global river networks over the 20th century

    NASA Astrophysics Data System (ADS)

    Vilmin, Lauriane; Mogollón, José M.; Beusen, Arthur H. W.; Bouwman, Alexander F.

    2018-04-01

    Nitrogen (N) and phosphorus (P) play a major role in the biogeochemical functioning of aquatic systems. N and P transfer to surface freshwaters has amplified during the 20th century, which has led to widespread eutrophication problems. The contribution of different sources, natural and anthropogenic, to total N and P loading to river networks has recently been estimated yearly using the Integrated Model to Assess the Global Environment - Global Nutrient Model (IMAGE-GNM). However, eutrophic events generally result from a combination of physicochemical conditions governed by hydrological dynamics and the availability of specific nutrient forms that vary at subyearly timescales. In the present study, we define for each simulated nutrient source: i) its speciation, and ii) its subannual temporal pattern. Thereby, we simulate the monthly loads of different N (ammonium, nitrate + nitrite, and organic N) and P forms (dissolved and particulate inorganic P, and organic P) to global river networks over the whole 20th century at a half-degree spatial resolution. Results indicate that, together with an increase in the delivery of all nutrient forms to global rivers, the proportion of inorganic forms in total N and P inputs has risen from 30 to 43% and from 56 to 65%, respectively. The high loads originating from fertilized agricultural lands and the increasing proportion of sewage inputs have led to a greater proportion of DIN forms (ammonium and nitrate), that are usually more bioavailable. Soil loss from agricultural lands, which delivers large amounts of particle-bound inorganic P to surface freshwaters, has become the dominant P source, which is likely to lead to an increased accumulation of legacy P in slow flowing areas (e.g., lakes and reservoirs). While the TN:TP ratio of the loads has remained quite stable, the DIN:DIP molar ratio, which is likely to affect algal development the most, has increased from 18 to 27 globally. Human activities have also affected the timing of nutrient delivery to surface freshwaters. Increasing wastewater emissions in growing urban areas induces constant local pressure on the quality of aquatic systems by delivering generally highly bioavailable nutrient forms, even in periods of low runoff.

  15. Role of the Small Intestine in Developmental Programming: Impact of Maternal Nutrition on the Dam and Offspring123

    PubMed Central

    Meyer, Allison M; Caton, Joel S

    2016-01-01

    Small-intestinal growth and function are critical for optimal animal growth and health and play a major role in nutrient digestion and absorption, energy and nutrient expenditure, and immunological competence. During fetal and perinatal development, the small intestine is affected by the maternal environment and nutrient intake. In ruminants, altered small-intestinal mass, villi morphology, hypertrophy, hyperplasia, vascularity, and gene expression have been observed as a result of poor gestational nutrition or intrauterine growth restriction. Although many of these data come from fetal stages, data have also demonstrated that nutrition during mid- and late gestation affects lamb small-intestinal growth, vascularity, digestive enzyme activity, and gene expression at 20 and 180 d of age as well. The small intestine is known to be a highly plastic tissue, changing with nutrient intake and physiological state even in adulthood, and the maternal small intestine adapts to pregnancy and advancing gestation. In ruminants, the growth, vascularity, and gene expression of the maternal small intestine also adapt to the nutritional plane and specific nutrient intake such as high selenium during pregnancy. These changes likely alter both pre- and postnatal nutrient delivery to offspring. More research is necessary to better understand the role of the offspring and maternal small intestines in whole-animal responses to developmental programming, but programming of this plastic tissue seems to play a dynamic role in gestational nutrition impacts on the whole animal. PMID:27180380

  16. Effects of Fe and Mn deficiencies on the protein profiles of tomato (Solanum lycopersicum) xylem sap as revealed by shotgun analyses.

    PubMed

    Ceballos-Laita, Laura; Gutierrez-Carbonell, Elain; Takahashi, Daisuke; Abadía, Anunciación; Uemura, Matsuo; Abadía, Javier; López-Millán, Ana Flor

    2018-01-06

    The aim of this work was to study the effects of Fe and Mn deficiencies on the xylem sap proteome of tomato using a shotgun proteomic approach, with the final goal of elucidating plant response mechanisms to these stresses. This approach yielded 643 proteins reliably identified and quantified with 70% of them predicted as secretory. Iron and Mn deficiencies caused statistically significant and biologically relevant abundance changes in 119 and 118 xylem sap proteins, respectively. In both deficiencies, metabolic pathways most affected were protein metabolism, stress/oxidoreductases and cell wall modifications. First, results suggest that Fe deficiency elicited more stress responses than Mn deficiency, based on the changes in oxidative and proteolytic enzymes. Second, both nutrient deficiencies affect the secondary cell wall metabolism, with changes in Fe deficiency occurring via peroxidase activity, and in Mn deficiency involving peroxidase, Cu-oxidase and fasciclin-like arabinogalactan proteins. Third, the primary cell wall metabolism was affected by both nutrient deficiencies, with changes following opposite directions as judged from the abundances of several glycoside-hydrolases with endo-glycolytic activities and pectin esterases. Fourth, signaling pathways via xylem involving CLE and/or lipids as well as changes in phosphorylation and N-glycosylation also play a role in the responses to these stresses. Biological significance In spite of being essential for the delivery of nutrients to the shoots, our knowledge of xylem responses to nutrient deficiencies is very limited. The present work applies a shotgun proteomic approach to unravel the effects of Fe and Mn deficiencies on the xylem sap proteome. Overall, Fe deficiency seems to elicit more stress in the xylem sap proteome than Mn deficiency, based on the changes measured in proteolytic and oxido-reductase proteins, whereas both nutrients exert modifications in the composition of the primary and secondary cell wall. Cell wall modifications could affect the mechanical and permeability properties of the xylem sap vessels, and therefore ultimately affect solute transport and distribution to the leaves. Results also suggest that signaling cascades involving lipid and peptides might play a role in nutrient stress signaling and pinpoint interesting candidates for future studies. Finally, both nutrient deficiencies seem to affect phosphorylation and glycosylation processes, again following an opposite pattern. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. Multiscale modelling and nonlinear simulation of vascular tumour growth

    PubMed Central

    Macklin, Paul; Anderson, Alexander R. A.; Chaplain, Mark A. J.; Cristini, Vittorio

    2011-01-01

    In this article, we present a new multiscale mathematical model for solid tumour growth which couples an improved model of tumour invasion with a model of tumour-induced angiogenesis. We perform nonlinear simulations of the multi-scale model that demonstrate the importance of the coupling between the development and remodeling of the vascular network, the blood flow through the network and the tumour progression. Consistent with clinical observations, the hydrostatic stress generated by tumour cell proliferation shuts down large portions of the vascular network dramatically affecting the flow, the subsequent network remodeling, the delivery of nutrients to the tumour and the subsequent tumour progression. In addition, extracellular matrix degradation by tumour cells is seen to have a dramatic affect on both the development of the vascular network and the growth response of the tumour. In particular, the newly developing vessels tend to encapsulate, rather than penetrate, the tumour and are thus less effective in delivering nutrients. PMID:18781303

  18. Toward understanding mechanisms controlling urea delivery in a coastal plain watershed

    USDA-ARS?s Scientific Manuscript database

    Improved understanding of nutrient mobilization and delivery to surface waters is critical to protecting water quality in agricultural watersheds. Urea, a form of organic nitrogen, is a common nutrient found in fertilizers, manures, and human waste, and is gaining recognition as an important driver ...

  19. Stream nitrate responses to hydrological forcing and climate change in northern forests of the USA (Invited)

    NASA Astrophysics Data System (ADS)

    Sebestyen, S. D.; Campbell, J. L.; Shanley, J. B.; Pourmokhtarian, A.; Driscoll, C. T.; Boyer, E. W.

    2009-12-01

    There is a need to understand how climate variability and change affect nutrient delivery to surface waters. We analyzed long-term records of hydrochemical data to explore how the forms, concentrations, and loadings of nitrogen in forest streams throughout the northern USA vary with catchment wetness. We considered projected changes in growing season length and precipitation patterns to simulate future climate scenarios and to assess how stream nitrate loading responds to hydrological forcing under different climate change scenarios. At the Sleepers River Research Watershed in northeastern Vermont, model results suggest that stream nutrient loadings over the next century will respond to hydrological forcing during climate change that affects the amount of water that flows through the landscape. For example, growing season stream water yield (+20%) and nitrate loadings (+57%) increase in response to greater amounts of precipitation (+28%) during a warmer climate with a longer growing season (+43 days). We further explore these findings by presenting model results from a biogeochemical process model (PnET-BGC) to separate changes that are due to biogeochemical cycling and the effects of hydrological forcing. Our findings suggest that nitrogen cycling and transport will intensify during anthropogenic climate forcing, thereby affecting the timing and magnitude of annual stream nutrient loadings in northern forests of the USA.

  20. Data-driven nutrient analysis and reality check: Human inputs, catchment delivery and management effects

    NASA Astrophysics Data System (ADS)

    Destouni, G.

    2017-12-01

    Measures for mitigating nutrient loads to aquatic ecosystems should have observable effects, e.g, in the Baltic region after joint first periods of nutrient management actions under the Baltic Sea Action Plan (BASP; since 2007) and the EU Water Framework Directive (WFD; since 2009). Looking for such observable effects, all openly available water and nutrient monitoring data since 2003 are compiled and analyzed for Sweden as a case study. Results show that hydro-climatically driven water discharge dominates the determination of waterborne loads of both phosphorus and nitrogen. Furthermore, the nutrient loads and water discharge are all similarly well correlated with the ecosystem status classification of Swedish water bodies according to the WFD. Nutrient concentrations, which are hydro-climatically correlated and should thus reflect human effects better than loads, have changed only slightly over the study period (2003-2013) and even increased in moderate-to-bad status waters, where the WFD and BSAP jointly target nutrient decreases. These results indicate insufficient distinction and mitigation of human-driven nutrient components by the internationally harmonized applications of both the WFD and the BSAP. Aiming for better general identification of such components, nutrient data for the large transboundary catchments of the Baltic Sea and the Sava River are compared. The comparison shows cross-regional consistency in nutrient relationships to driving hydro-climatic conditions (water discharge) for nutrient loads, and socio-economic conditions (population density and farmland share) for nutrient concentrations. A data-driven screening methodology is further developed for estimating nutrient input and retention-delivery in catchments. Its first application to nested Sava River catchments identifies characteristic regional values of nutrient input per area and relative delivery, and hotspots of much larger inputs, related to urban high-population areas.

  1. Controls of event-based nutrient transport within nested headwater agricultural watersheds of the western Lake Erie basin

    NASA Astrophysics Data System (ADS)

    Williams, Mark R.; Livingston, Stanley J.; Penn, Chad J.; Smith, Douglas R.; King, Kevin W.; Huang, Chi-hua

    2018-04-01

    Understanding the processes controlling nutrient delivery in headwater agricultural watersheds is essential for predicting and mitigating eutrophication and harmful algal blooms in receiving surface waters. The objective of this study was to elucidate nutrient transport pathways and examine key components driving nutrient delivery processes during storm events in four nested agricultural watersheds (298-19,341 ha) in the western Lake Erie basin with poorly drained soils and an extensive artificial drainage network typical of the Midwestern U.S. Concentration-discharge hysteresis patterns of nitrate-nitrogen (NO3-N), dissolved reactive phosphorus (DRP), and particulate phosphorus (PP) occurring during 47 storm events over a 6 year period (2004-2009) were evaluated. An assessment of the factors producing nutrient hysteresis was completed following a factor analysis on a suite of measured environmental variables representing the fluvial and wider watershed conditions prior to, and during the monitored storm events. Results showed the artificial drainage network (i.e., surface tile inlets and subsurface tile drains) in these watersheds was the primary flow pathway for nutrient delivery to streams, but nutrient behavior and export during storm events was regulated by the flow paths to and the intensity of the drainage network, the availability of nutrients, and the relative contributions of upland and in-stream nutrient sources. Potential sources and flow pathways for transport varied among NO3-N, PP, and DRP with results underscoring the challenge of mitigating nutrient loss in these watersheds. Conservation practices addressing both nutrient management and hydrologic connectivity will likely be required to decrease nutrient loss in artificially drained landscapes.

  2. Nutrient export from watersheds on Mt. Desert Island, maine, as a function of land use and fire history

    USGS Publications Warehouse

    Nielsen, M.G.; Kahl, J.S.

    2007-01-01

    A study of 13 small (less than 7.5 km2) watersheds on Mt. Desert Island, Maine, was conducted from January 1999 to September 2000 to determine nutrient export delivery to coastal waters around the island, and to determine whether a series of wildfires in 1947 have affected nutrient export in burned watersheds. Nutrient export (nitrate-nitrogen, total nitrogen, total phosphorus) was determined for each watershed during the study period, and was normalized by watershed area. The yield of nitrate-nitrogen (N) ranged from 10 to 140 kg/km2/year. Total N yield ranged from 42 to 250 kg/ km2/year. Total phosphorus (P) yield ranged from 1.4 to 7.9 kg/km2/year. Watersheds entirely within Acadia National Park (lacking human land-based nutrient sources) exported significantly less total N and total P than watersheds that were partly or entirely outside the park boundary. Nitrate-N export was not significantly different in these two groups of watersheds, perhaps because atmospheric deposition is a dominant source of nitrate in the study area. No relation was observed between burn history and nutrient export. Any effect of burn history may be masked by other landscape-level factors related to nutrient export. ?? Springer Science + Business Media B.V. 2007.

  3. A proposal to demonstrate production of salad crops in the Space Station Mockup Facility with particular attention to space, energy, and labor constraints

    NASA Technical Reports Server (NTRS)

    Brooks, Carolyn A.

    1992-01-01

    The Salad Machine Research has continued to be a two path effort with the research at Marshall Space Flight Center (MSFC) focusing on the design, construction, and operation of a semiautomated system (Salad Machine) for the production of salad vegetables within a standard rack. Boeing Corporation in cooperation with NASA MSFC constructed a four drawer Salad Machine which was occasionally placed within the Space Station Freedom Mockup facility for view by selected visitors. Final outfitting of the Salad Machine is awaiting the arrival of parts for the nutrient delivery system. Research at the Alabama A&M facilities focused on compatibility of radish and lettuce plants when grown on the same nutrient solution. Lettuce fresh weight shoot yield was significantly enhanced when lettuce plants were grown on nutrient solution which was shared with radish. Radish tuber production was not significantly affected although there was a trend for radish from shared solutions to be heavier than those grown on separate nutrient solutions. The effect of sharing nutrient solutions on carbohydrate partitioning reflected the effect of sharing solution on fresh weight yield. Lettuce shoot dry weight was significantly greater for plants from shared solutions than from separate. There was no significant effect on sharing nutrient solution on radish tuber dry weight. Partitioning of nitrogen, calcium, magnesium, and potassium was not affected by sharing, there was, however, a disproportionate amount of potassium in the tissues, suggesting luxury consumption of potassium in all plants and tissues. It is concluded that lettuce plants benefit from sharing nutrient solution with radish and that radish is not harmed.

  4. Porous tube plant nutrient delivery system development: A device for nutrient delivery in microgravity

    NASA Technical Reports Server (NTRS)

    Dreschel, T. W.; Brown, C. S.; Piastuch, W. C.; Hinkle, C. R.; Knott, W. M.

    1994-01-01

    The Porous Tube Plant Nutrient Delivery Systems or PTPNDS (U.S. Patent #4,926,585) has been under development for the past six years with the goal of providing a means for culturing plants in microgravity, specifically providing water and nutrients to the roots. Direct applications of the PTPNDS include plant space biology investigations on the Space Shuttle and plant research for life support in the Space Station Freedom. In the past, we investigated various configurations, the suitability of different porous materials, and the effects of pressure and pore size on plant growth. Current work is focused on characterizing the physical operation of the system, examining the effects of solution aeration, and developing prototype configurations for the Plant Growth Unit (PGU), the flight system for the Shuttle mid-deck. Future developments will involve testing on KC-135 parabolic flights, the design of flight hardware and testing aboard the Space Shuttle.

  5. Real-time monitoring of nutrients in the Changjiang Estuary reveals short-term nutrient-algal bloom dynamics

    NASA Astrophysics Data System (ADS)

    Wang, Kui; Chen, Jianfang; Ni, Xiaobo; Zeng, Dingyong; Li, Dewang; Jin, Haiyan; Glibert, Patricia M.; Qiu, Wenxian; Huang, Daji

    2017-07-01

    The Changjiang Estuary is a large-river estuary ecosystem in the East China Sea, and its plume, the Changjiang Diluted Water (CDW), transports a large mass of nutrients (NO3- + NO2-, PO43-, SiO32-) to the shelf sea, leading to substantial eutrophication; the CDW also supports high primary production. However, relationships between nutrient delivery and phytoplankton responses have been difficult to establish, as many nutrient delivery events and algal blooms are episodic, and the CDW may expand or become detached with changing winds. To study the relationship between nutrient delivery events, algal blooms and estuarine metabolism dynamics, a buoy system was deployed in the CDW from 9 September to 10 October 2013, with measurements of chlorophyll a and dissolved nutrients. Day-to-day nutrient increases covaried with salinity decreases, regulated by both the spring-neap tidal cycle and wind events. Several specific nutrient injection periods were detected, each followed by nutrient drawdown and chlorophyll a accumulation (algal blooms). Each algal bloom had its own unique pattern of nutrient uptake based on change in nutrient ratios (ΔN:ΔP; ΔN:ΔSi) and appeared to be dominated by different algal groups. These events occurred under weak wind and stable hydrodynamic conditions. Ecosystem metabolism based on net community production (NCP) showed that the upper estuarine ecosystem was autotrophic when chlorophyll a accumulated, but heterotrophic when wind-induced mixing strengthened, and upwelling brought organic-rich water to the near surface. In spite of several short-lived algal blooms, the average NCPdaily was negative during the observation period, indicating a net source of CO2 to the atmosphere.

  6. Atmosphere-Forest Exchange: Important Questions Regarding the Atmosphere's Role in the Delivery of Nutrient Nitrogen and Impacts on Nitrogen and Carbon Cycling

    NASA Astrophysics Data System (ADS)

    Carroll, M.; Shepson, P. B.; Bertman, S. B.; Sparks, J. P.; Holland, E. A.

    2002-12-01

    Atmosphere-Forest Exchange: Important Questions Regarding the Atmosphere's Role in the Delivery of Nutrient Nitrogen and Impacts on Nitrogen and Carbon Cycling Atmospheric composition and chemistry directly affect ecosystem nitrogen cycling and indirectly affect ecosystem carbon cycling and storage. Current understanding of atmosphere-forest nitrogen exchange and subsequent impacts is based almost exclusively on nitrogen deposition data obtained from networks using buckets placed in open areas, studies involving inorganic nitrogen, frequently with enhanced N deposition inputs applied only to soils, and that ignore multiple stresses (e.g., the combined effects of aerosols, ozone exposure, elevated CO2, and drought). Current models of nitrogen cycling treat deposited nitrogen (e.g., HNO3 and NO3-) as a permanent sink whereas data appear to indicate that photolytic and heterogeneous chemical processes occurring on surfaces and in dew can result in the re-evolution of gaseous species such as NO and HONO. Similarly, the direct uptake of gaseous nitrogen compounds by foliage has been neglected, compromising conclusions drawn from deposition experiments and ignoring a mechanism that may significantly affect nitrogen cycling and carbon storage, one that may become more significant with future atmospheric and climate change. We hypothesize that the atmosphere plays a significant role in the delivery of nutrient nitrogen to the N-limited mixed hardwood forest at the PROPHET research site at the University of Michigan Biological Station. We assert that a complete understanding of atmosphere- biosphere interactions and feedbacks is required to develop a predictive capability regarding forest response to increasing atmospheric CO2, reactive nitrogen, oxidants, and aerosols, increasing nitrogen and acidic deposition, and anticipated climate change. We further assert that conclusions drawn from studies that are limited to inorganic nitrogen, fertilization of soils, and/or that neglect the role of the canopy (in N uptake and/or remobilization) may not produce a complete understanding of N and C cycling in terrestrial ecosystems, including atmosphere-biosphere interactions and feedbacks. Here, as part of a new PROPHET focus on Biosphere Exchange of Atmospheric Carbon and Odd Nitrogen (BEACON), we identify a number of issues associated with nitrogen limited forest ecosystems and nitrogen saturation and important science questions that require collaborative studies involving the atmospheric and biospheric science communities.

  7. The mechanism of diabetes control after gastrointestinal bypass surgery reveals a role of the proximal small intestine in the pathophysiology of type 2 diabetes.

    PubMed

    Rubino, Francesco; Forgione, Antonello; Cummings, David E; Vix, Michel; Gnuli, Donatella; Mingrone, Geltrude; Castagneto, Marco; Marescaux, Jacques

    2006-11-01

    Most patients who undergo Roux-en-Y gastric bypass (RYGB) experience rapid resolution of type 2 diabetes. Prior studies indicate that this results from more than gastric restriction and weight loss, implicating the rearranged intestine as a primary mediator. It is unclear, however, if diabetes improves because of enhanced delivery of nutrients to the distal intestine and increased secretion of hindgut signals that improve glucose homeostasis, or because of altered signals from the excluded segment of proximal intestine. We sought to distinguish between these two mechanisms. Goto-Kakizaki (GK) type 2 diabetic rats underwent duodenal-jejunal bypass (DJB), a stomach-preserving RYGB that excludes the proximal intestine, or a gastrojejunostomy (GJ), which creates a shortcut for ingested nutrients without bypassing any intestine. Controls were pair-fed (PF) sham-operated and untreated GK rats. Rats that had undergone GJ were then reoperated to exclude the proximal intestine; and conversely, duodenal passage was restored in rats that had undergone DJB. Oral glucose tolerance (OGTT), food intake, body weight, and intestinal nutrient absorption were measured. There were no differences in food intake, body weight, or nutrient absorption among surgical groups. DJB-treated rats had markedly better oral glucose tolerance compared with all control groups as shown by lower peak and area-under-the-curve glucose values (P < 0.001 for both). GJ did not affect glucose homeostasis, but exclusion of duodenal nutrient passage in reoperated GJ rats significantly improved glucose tolerance. Conversely, restoration of duodenal passage in DJB rats reestablished impaired glucose tolerance. This study shows that bypassing a short segment of proximal intestine directly ameliorates type 2 diabetes, independently of effects on food intake, body weight, malabsorption, or nutrient delivery to the hindgut. These findings suggest that a proximal intestinal bypass could be considered for diabetes treatment and that potentially undiscovered factors from the proximal bowel might contribute to the pathophysiology of type 2 diabetes.

  8. Numerical modeling of the fetal blood flow in the placental circulatory system

    NASA Astrophysics Data System (ADS)

    Shannon, Alexander; Gallucci, Sergio; Mirbod, Parisa

    2015-11-01

    The placenta is a unique organ of exchange between the growing fetus and the mother. It incorporates almost all functions of the adult body, acting as the fetal lung, digestive and immune systems, to mention a few. The exchange of oxygen and nutrients takes place at the surface of the villous tree. Using an idealized geometry of the fetal villous trees in the mouse placenta, in this study we performed 3D computational analysis of the unsteady fetal blood flow, gas, and nutrient transport over the chorionic plate. The fetal blood was treated as an incompressible Newtonian fluid, and the oxygen and nutrient were treated as a passive scalar dissolved in blood plasma. The flow was laminar, and a commercial CFD code (COMSOL Multiphysics) has been used for the simulation. COMSOL has been selected because it is multi-physics FEM software that allows for the seamless coupling of different physics represented by partial differential equations. The results clearly illustrate that the specific branching pattern and the in-plane curvature of the fetal villous trees affect the delivery of blood, gas and nutrient transport to the whole placenta.

  9. A summary of porous tube plant nutrient delivery system investigations from 1985 to 1991

    NASA Technical Reports Server (NTRS)

    Dreschel, T. W.; Brown, C. S.; Piastuch, W. C.; Hinkle, C. R.; Sager, J. C.; Wheeler, R. M.; Knott, W. M.

    1992-01-01

    The Controlled Ecological Life Support System (CELSS) Program is a research effort to evaluate biological processes at a one person scale to provide air, water, and food for humans in closed environments for space habitation. This program focuses currently on the use of conventional crop plants and the use of hydroponic systems to grow them. Because conventional hydroponic systems are dependent on gravity to conduct solution flow, they cannot be used in the microgravity of space. Thus, there is a need for a system that will deliver water and nutrients to plant roots under microgravity conditions. The Plant Space Biology Program is interested in investigating the effect that the space environment has on the growth and development of plants. Thus, there is also a need to have a standard nutrient delivery method for growing plants in space for research into plant responses to microgravity. The Porous Tube Plant Nutrient Delivery System (PTPNDS) utilizes a hydrophilic, microporous material to control water and nutrient delivery to plant roots. It has been designed and analyzed to support plant growth independent of gravity and plans are progressing to test it in microgravity. It has been used successfully to grow food crops to maturity in an earth-bound laboratory. This document includes a bibliography and summary reports from the growth trials performed utilizing the PTPNDS.

  10. Nutrient processes at the stream-lake interface for a channelized versus unmodified stream mouth

    USGS Publications Warehouse

    Niswonger, Richard G.; Naranjo, Ramon C.; Smith, David; Constantz, James E.; Allander, Kip K.; Rosenberry, Donald O.; Neilson, Bethany; Rosen, Michael R.; Stonestrom, David A.

    2017-01-01

    Inorganic forms of nitrogen and phosphorous impact freshwater lakes by stimulating primary production and affecting water quality and ecosystem health. Communities around the world are motivated to sustain and restore freshwater resources and are interested in processes controlling nutrient inputs. We studied the environment where streams flow into lakes, referred to as the stream-lake interface (SLI), for a channelized and unmodified stream outlet. Channelization is done to protect infrastructure or recreational beach areas. We collected hydraulic and nutrient data for surface water and shallow groundwater in two SLIs to develop conceptual models that describe characteristics that are representative of these hydrologic features. Water, heat, and solute transport models were used to evaluate hydrologic conceptualizations and estimate mean residence times of water in the sediment. A nutrient mass balance model is developed to estimate net rates of adsorption and desorption, mineralization, and nitrification along subsurface flow paths. Results indicate that SLIs are dynamic sources of nutrients to lakes and that the common practice of channelizing the stream at the SLI decreases nutrient concentrations in pore water discharging along the lakeshore. This is in contrast to the unmodified SLI that forms a barrier beach that disconnects the stream from the lake and results in higher nutrient concentrations in pore water discharging to the lake. These results are significant because nutrient delivery through pore water seepage at the lakebed from the natural SLI contributes to nearshore algal communities and produces elevated concentrations of inorganic nutrients in the benthic zone where attached algae grow.

  11. Changes in Streamflow and the Flux of Nutrients in the Mississippi-Atchafalaya River Basin, USA, 1980-2007

    USGS Publications Warehouse

    Battaglin, William A.; Aulenbach, Brent T.; Vecchia, Aldo; Buxton, Herbert T.

    2010-01-01

    Nutrients and freshwater delivered by the Mississippi and Atchafalaya Rivers drive algal production in the northern Gulf of Mexico, which eventually results in the widespread occurrence of hypoxic bottom waters along the Louisiana and Texas coast. Researchers have demonstrated a relation between the extent of the hypoxic zone and the magnitude of streamflow, nutrient fluxes, and nutrient concentrations in the Mississippi River, with springtime streamflows and fluxes being the most predictive. In 1999 the U.S. Geological Survey (USGS) estimated the flux of nitrogen, phosphorus, and silica at selected sites in the Mississippi Basin and to the Gulf of Mexico for 1980-1996. These flux estimates provided the baseline information used by the Mississippi River/Gulf of Mexico Watershed Nutrient Task Force to develop an Action Plan for reducing hypoxia in the northern Gulf of Mexico. The primary goal of the Action Plan was to achieve a reduction in the size (areal extent) of the hypoxic zone from an average of approximately 14,000 square kilometers in 1996-2000 to a 5-year moving average of less than 5,000 square kilometers by 2015. Improved statistical models and adjusted maximum likelihood estimation using USGS Load Estimator (LOADEST) software were used to estimate annual and seasonal nutrient fluxes for 1980-2007 at selected sites on the Mississippi River and its tributaries. These data provide a means to evaluate the influence of natural and anthropogenic effects on delivery of water and nutrients to the Gulf of Mexico; to define subbasins that are the most important contributors of nutrients to the gulf; and to investigate the relations among streamflow, nutrient fluxes, and the size and duration of the Gulf of Mexico hypoxic zone. A comparative analysis between the baseline period of 1980-1996 and 5-year moving averages thereafter indicate that the average annual streamflow and fluxes of total nitrogen, nitrate, orthophosphate, and silica to the Gulf of Mexico have decreased. However, the flux of total phosphorus between the baseline period and subsequent 5-year periods has increased. The average spring (April, May, and June) streamflow and fluxes of silica, total nitrogen, nitrate, and orthophosphate to the Gulf of Mexico also decreased, whereas the spring flux of total phosphorus has increased. Similar changes in streamflow and nutrient flux were observed at many sites Buxtonwithin the basin. The inputs of water, total nitrogen, and total phosphorus from the major subbasins of the Mississippi-Atchafalaya River Basin as a percentage of the to-the-gulf totals have increased from the Ohio River Basin, decreased from the Missouri River Basin, and remained relatively unchanged from the Upper Mississippi, Red, and Arkansas River Basins. Changes in streamflow and nutrient fluxes are related, but short-term variations in sources of streamflow and nutrients complicate the interpretation of factors that affect nutrient delivery to the Gulf of Mexico. Parametric time-series models are used to try and separate natural variability in nutrient flux from changes due to other causes. Results indicate that the decrease in annual nutrient fluxes that has occurred between the 1980-1996 baseline period and more recent years can be largely attributed to natural causes (climate and streamflow) and not management actions or other human controlled activities in the Mississippi-Atchafalaya River Basin. The downward trends in total nitrogen, nitrate, ammonium, and orthophosphate that were detected at either the Mississippi River near St. Francisville, La., or the Atchafalaya River at Melville, La., occurred prior to 1995. In spite of the general decrease in nutrient flux, the average size of the Gulf of Mexico hypoxic zone has increased between 1997 and 2007. The reasons for this are not clear but could be due to the type or nature of nutrient delivery. Whereas the annual flux of total nitrogen to the Gulf of Mexico has decreased, the proporti

  12. Assessment of changes in nutrient and sediment delivery to and carbon accumulation in coastal oceans of the Eastern United States

    NASA Astrophysics Data System (ADS)

    Bergamaschi, B. A.; Smith, R. A.; Shih, J. S.; Sohl, T. L.; Sleeter, B. M.; Zhu, Z.

    2014-12-01

    Land-use and land-cover distributions are primary determinants of terrestrial fluxes of sediments and nutrients to coastal oceans. Sediment and nutrient delivery to coastal waters have already been significantly altered by changes in population and land use, resulting in modified patterns of coastal production and carbon storage. Continued population growth and increasing agricultural areal extent and intensity are expected to accelerate these changes. The USGS LandCarbon project developed prospective future land use and land cover projections based on IPCC scenarios A1b, A2 and B1 to 2050 as the basis for a multitude of biogeochemical assessments. We assessed the impacts on delivery of nutrients and sediments to the coastal ocean, and concomitant carbon storage. Fluxes were estimated using the SPARROW model, calibrated on historical water quality measurements. Significantly greater fluxes of nutrients and sediments to coastal waters by 2050 are projected by the model. For example, for the Eastern United States, nitrate fluxes for 2050 are projected to be16 to 52 percent higher than the baseline year, depending on scenario. As a consequence, an associated increase in the frequency and duration of coastal and estuarine hypoxia events and harmful algal blooms could be expected. Model estimates indicate that these prospective future nutrient and sediment fluxes will increase carbon storage rates in coastal waters by 18 to 56 percent in some regions.

  13. Ecosystem effects in the Lower Mississippi River Basin: Chapter L in 2011 Floods of the Central United States

    USGS Publications Warehouse

    Turnipseed, D. Phil; Allen, Yvonne C.; Couvillion, Brady R.; McKee, Karen L.; Vervaeke, William C.

    2014-01-01

    The 2011 Mississippi River flood in the Lower Mississippi River Basin was one of the largest flood events in recorded history, producing the largest or next to largest peak streamflow for the period of record at a number of streamgages on the lower Mississippi River. Ecosystem effects include changes to wetlands, nutrient transport, and land accretion and sediment deposition changes. Direct effects to the wetland ecosystems in the Lower Mississippi River Basin were minimized because of the expansive levee system built to pass floodwaters. Nutrients carried by the Mississippi River affect water quality in the Lower Mississippi River Basin. During 2011, nutrient fluxes in the lower Mississippi River were about average. Generally, nutrient delivery of the Mississippi and Atchafalaya Rivers contributes to the size of the hypoxic zone in the Gulf of Mexico. Based on available limited post-flood satellite imagery, some land expansion in both the Wax Lake and Atchafalaya River Deltas was observed. A wetland sediment survey completed in June 2011 indicated that recent sediment deposits were relatively thicker in the Atchafalaya and Mississippi River (Birdsfoot) Delta marshes compared to marshes farther from these rivers.

  14. Slow-Release Fertilizer

    NASA Technical Reports Server (NTRS)

    1998-01-01

    Under an SBIR (Small Business Innovative Research), ZeoponiX, Inc., introduced ZeoPro. This product is used as a fertilizer/soil amendment for golf courses, ball fields, greenhouse and horticultural uses. A combination of superior growth medium and soil conditioner allow for nutrient supplementation and high efficiency delivery of nutrients throughout the plant. ZeoPro provides a balanced nutrient system for major, minor, and trace nutrients.

  15. Benthic Food Webs of the Chukchi and Beaufort Seas: Relative Importance of Ultimate Carbon Sources in a Changing Climate

    NASA Astrophysics Data System (ADS)

    Dunton, K. H.; Schonberg, S. V.; Mctigue, N.; Bucolo, P. A.; Connelly, T. L.; McClelland, J. W.

    2014-12-01

    Changes in sea-ice cover, coastal erosion, and freshwater run-off have the potential to greatly influence carbon assimilation pathways and affect trophic structure in benthic communities across the western Arctic. In the Chukchi Sea, variations in the duration and timing of ice cover affect the delivery of ice algae to a relatively shallow (40-50 m) shelf benthos. Although ice algae are known as an important spring carbon subsidy for marine benthic fauna, ice algal contributions may also help initiate productivity of an active microphytobenthos. Recent studies provide clear evidence that the microphytobenthos are photosynthetically active, and have sufficient light and nutrients for in situ growth. The assimilation of benthic diatoms from both sources may explain the 13C enrichment observed in benthic primary consumers throughout the northern Chukchi. On the eastern Beaufort Sea coast, shallow (2-4 m) estuarine lagoon systems receive massive subsidies of terrestrial carbon that is assimilated by a benthic fauna of significant importance to upper trophic level species, but again, distinct 13C enrichment in benthic primary consumers suggests the existence of an uncharacterized food source. Since ice algae are absent, we believe the 13C enrichment in benthic fauna is caused by the assimilation of benthic microalgae, as reflected in seasonally high benthic chlorophyll in spring under replete light and nutrient conditions. Our observations suggest that changes in ice cover, on both temporal and spatial scales, are likely to have significant effects on the magnitude and timing of organic matter delivery to both shelf and nearshore systems, and that locally produced organic matter may become an increasingly important carbon subsidy that affects trophic assimilation and secondary ecosystem productivity.

  16. Feed-Back Moisture Sensor Control for the Delivery of Water to Plants Cultivated in Space

    NASA Technical Reports Server (NTRS)

    Levine, Howard G.; Prenger, Jessica J.; Rouzan, Donna T.; Spinale, April C.; Murdoch, Trevor; Burtness, Kevin A.

    2005-01-01

    The development of a spaceflight-rated Porous Tube Insert Module (PTIM) nutrient delivery tray has facilitated a series of studies evaluating various aspects of water and nutrient delivery to plants as they would be cultivated in space. We report here on our first experiment using the PTIM with a software-driven feedback moisture sensor control strategy for maintaining root zone wetness level set-points. One-day-old wheat seedlings (Tritium aestivum cv Apogee; N=15) were inserted into each of three Substrate Compartments (SCs) pre-packed with 0.25-1 . mm Profile(TradeMark) substrate and maintained at root zone relative water content levels of 70, 80 and 90%. The SCs contained a bottom-situated porous tube around which a capillary mat was wrapped. Three Porous Tubes. were planted using similar protocols (but without the substrate) and also maintained at these three moisture level set-points. Half-strength modified Hoagland's nutrient solution was used to supply water and nutrients. Results on hardware performance, water usage rates and wheat developmental differences between the different experimental treatments are presented.

  17. Controls of event-based nutrient transport within nested headwater agricultural watersheds of the western Lake Erie basin

    USDA-ARS?s Scientific Manuscript database

    Understanding the processes controlling nutrient delivery in headwater agricultural watersheds is essential for predicting and mitigating eutrophication and harmful algal blooms in receiving surface waters. The objective of this study was to elucidate nutrient transport pathways and examine key comp...

  18. Fish-derived nutrient hotspots shape coral reef benthic communities.

    PubMed

    Shantz, Andrew A; Ladd, Mark C; Schrack, Elizabeth; Burkepile, Deron E

    2015-12-01

    Animal-derived nutrients play an important role in structuring nutrient regimes within and between ecosystems. When animals undergo repetitive, aggregating behavior through time, they can create nutrient hotspots where rates of biogeochemical activity are higher than those found in the surrounding environment. In turn, these hotspots can influence ecosystem processes and community structure. We examined the potential for reef fishes from the family Haemulidae (grunts) to create nutrient hotspots and the potential impact of these hotspots on reef communities. To do so, we tracked the schooling locations of diurnally migrating grunts, which shelter at reef sites during the day but forage off reef each night, and measured the impact of these fish schools on benthic communities. We found that grunt schools showed a high degree of site fidelity, repeatedly returning to the same coral heads. These aggregations created nutrient hotspots around coral heads where nitrogen and phosphorus delivery was roughly 10 and 7 times the respective rates of delivery to structurally similar sites that lacked schools of these fishes. In turn, grazing rates of herbivorous fishes at grunt-derived hotspots were approximately 3 times those of sites where grunts were rare. These differences in nutrient delivery and grazing led to distinct benthic communities with higher cover of crustose coralline algae and less total algal abundance at grunt aggregation sites. Importantly, coral growth was roughly 1.5 times greater at grunt hotspots, likely due to the important nutrient subsidy. Our results suggest that schooling reef fish and their nutrient subsidies play an important role in mediating community structure on coral reefs and that overfishing may have important negative consequences on ecosystem functions. As such, management strategies must consider mesopredatory fishes in addition to current protection often offered to herbivores and top-tier predators. Furthermore, our results suggest that restoration strategies may benefit from focusing on providing structure for aggregating fishes on reefs with low topographic complexity or focusing the restoration of nursery raised corals around existing nutrient hotspots.

  19. Delivery and Establishing Slow Release Carbon Source to the Hanford Vadose Zone Using Colloidal Silica Suspension Injection and Subsequent Gelation - Laboratory Study

    NASA Astrophysics Data System (ADS)

    Zhong, L.; Lee, M. H.; Lee, B.; Yang, S.

    2016-12-01

    Delivery of nutrient to and establish a slow release carbon source in the vadose zone and capillary fringe zone is essential for setting up of a long-lasting bioremediation of contaminations in those zones. Conventional solution-based injection and infiltration approaches are facing challenges to achieve the delivery and remedial goals. Aqueous silica suspensions undergo a delayed gelation process under favorite geochemical conditions. The delay in gelation provides a time window for the injection of the suspension into the subsurface; and the gelation of the amendment-silica suspension enables the amendment-laden gel to stay in the target zone and slowly release the constituents for contaminant remediation. This approach can potentially be applied to deliver bio-nutrients to the vadose zone and capillary fringe zone for enhanced bioremediation and achieve remedial goals. This research was conducted to demonstrate delayed gelation of colloidal silica suspensions when carbon sources were added and to prove the gelation occurs in sediments under vadose conditions. Sodium lactate, vegetable oil, ethanol, and molasses were tested as the examples of carbon source (or nutrient) amendments. The rheological properties of the silica suspensions during the gelation were characterized. The influence of silica, salinity, nutrient concentrations, and the type of nutrients was studied. The kinetics of nutrient release from silica-nutrient gel was quantified using molasses as the example, and the influence of suspension gelation time was evaluated. The injection behavior of the suspensions was investigated by monitoring their viscosity changes and the injection pressures when the suspensions were delivered into sediment columns.

  20. Malnutrition in the Critically Ill Child: The Importance of Enteral Nutrition

    PubMed Central

    Prieto, Marta Botrán; Cid, Jesús López-Herce

    2011-01-01

    Malnutrition affects 50% of hospitalized children and 25–70% of the critically ill children. It increases the incidence of complications and mortality. Malnutrition is associated with an altered metabolism of certain substrates, increased metabolism and catabolism depending on the severity of the lesion, and reduced nutrient delivery. The objective should be to administer individualized nutrition to the critically ill child and to be able to adjust the nutrition continuously according to the metabolic changes and evolving nutritional status. It would appear reasonable to start enteral nutrition within the first 24 to 48 hours after admission, when oral feeding is not possible. Parenteral nutrition should only be used when enteral nutrition is contraindicated or is not tolerated. Energy delivery must be individually adjusted to energy expenditure (40–65 kcal/100 calories metabolized/day) with a protein delivery of 2.5–3 g/kg/day. Frequent monitoring of nutritional and metabolic parameters should be performed. PMID:22163211

  1. Plant Growth Module (PGM) conceptual design

    NASA Technical Reports Server (NTRS)

    Schwartzkopf, Steven H.; Rasmussen, Daryl

    1987-01-01

    The Plant Growth Module for the Controlled Ecological Life Support System (CELSS), designed to answer basic science questions related to growing plants in closed systems, is described functionally with artist's conception drawings. Subsystems are also described, including enclosure and access; data acquisition and control; gas monitor and control; heating, ventilation, and air conditioning; air delivery; nutrient monitor and control; microbial monitoring and control; plant support and nutrient delivery; illumination; and internal operations. The hardware development plan is outlined.

  2. Conceptual design of a closed loop nutrient solution delivery system for CELSS implementation in a micro-gravity environment

    NASA Technical Reports Server (NTRS)

    Schwartzkopf, Steven H.; Oleson, Mel W.; Cullingford, Hatice S.

    1990-01-01

    Described here are the results of a study to develop a conceptual design for an experimental closed loop fluid handling system capable of monitoring, controlling, and supplying nutrient solution to higher plants. The Plant Feeder Experiment (PFE) is designed to be flight tested in a microgravity environment. When flown, the PFX will provide information on both the generic problems of microgravity fluid handling and the specific problems associated with the delivery of the nutrient solution in a microgravity environment. The experimental hardware is designed to fit into two middeck lockers on the Space Shuttle, and incorporates several components that have previously been flight tested.

  3. Removal of nutrient limitations by long-term fertilization decreases nocturnal water loss in savanna trees.

    Treesearch

    F.G. Scholz; S.J. Bucci; G. Goldstein; F.C. Meinzer; A.C. Franco; F. Miralles-Wilhelm

    2007-01-01

    Under certain environmental conditions, nocturnal transpiration can be relatively high in temperate and tropical woody species. In nutrient-poor systems such as the Brazilian Cerrado, nocturnal transpiration may enhance delivery of nutrients to roots. We compared nocturnal transpiration of three dominant Cerrado tree species growing in unfertilized plots and plots to...

  4. Feed handling of lactating crossbred cows maintained in a semi-arid region during the hot season: physiological parameters, ingestive behavior and performance.

    PubMed

    de Paula Xavier de Andrade, Rafael; de Andrade Ferreira, Marcelo; de Azevedo, Marcílio; da Silva, Emmanuelle Cordeiro; Urbano, Stela Antas; da Conceição, Maria Gabriela; de Lima Silva, Janaina

    2017-01-01

    The effects of time of feed delivery (14.00 hours; 14.00 and 20.00 hours; 16.00 and 18.00 hours) on the physiological parameters, ingestive behavior, nutrient intake and production of lactating cows maintained in a semi-arid region during the hot season were evaluated. Regardless of treatment, all animals received the first feeding supply at 06.00 hours. Eight cows with an average body weight of 600 kg, average milk yield of 20 kg/day and 80 days in milk were utilized. The rectal temperature, respiratory rate and sweating rate were not affected (P > 0.05), with average values of 38.5°C, 53.8 movements/min and 104 g/m 2 /h respectively. There was no effect (P > 0.05) on the eating time (314 min/day), ruminating time (564 min/day), drinking time (61 min/day) and idle time (502 min/day). Similarly, the intake of nutrients and performance of lactating cows were not affected (P > 0.05), with average dry matter intake of 19.8 kg/day, 4% fat-corrected milk of 20.6 kg/day and milk fat concentration of 4.03 g/100 g. Since the behavior and performance have not altered, any times of feed delivery evaluated could be used to crossbred Holstein × Zebu cows maintained on a feedlot in semi-arid regions during the hot season. © 2016 Japanese Society of Animal Science.

  5. Digital data used to relate nutrient inputs to water quality in the Chesapeake Bay watershed, version 3.0

    USGS Publications Warehouse

    Brakebill, John W.; Preston, Stephen D.

    2004-01-01

    Chesapeake Bay restoration efforts are focused on improving water quality, living resources, and ecological habitats by 2010. One aspect of the water-quality restoration is the refinement of strategies designed to implement nutrient-reduction practices within the Bay watershed. These strategies are being refined and implemented by resource managers of the Chesapeake Bay Program (CBP), a partnership comprised of various Federal, State, and local agencies that includes jurisdictions within Delaware, Maryland, New York, Pennsylvania, Virginia, West Virginia, and the District of Columbia. The U.S. Geological Survey (USGS), an active member of the CBP, provides necessary water-quality information for these Chesapeake Bay nutrient-reduction strategy revisions and evaluations. The formulation and revision of effective nutrient-reduction strategies requires detailed scientific information and an analytical understanding of the sources, transport, and delivery of nutrients to the Chesapeake Bay. The USGS is supporting these strategies by providing scientific information to resource managers that can help them evaluate and understand these processes. One statistical model available to resource managers is a collection of SPAtially Referenced Regressions On Watershed (SPARROW) attributes, which uses a nonlinear regression approach to spatially relate nutrient sources and watershed characteristics to nutrient loads of streams throughout the Chesapeake Bay watershed. Developed by the USGS, information generated by SPARROW can help resource managers determine the geographical distribution and relative contribution of nutrient sources and the factors that affect their transport to the Bay. Nutrient source information representing the late 1990s time period was obtained from several agencies and used to create and compile digital spatial datasets of total nitrogen and total phosphorus contributions that served as input sources to the SPARROW models. These data represent atmospheric deposition, point-source locations, land-use, land-cover, and agricultural sources such as commercial fertilizer and manure applications. Watershed-characteristics datasets representing factors that affect the transport of nutrients also were compiled from previous applications of the SPARROW models in the Chesapeake Bay watershed. Datasets include average-annual precipitation and temperature, slope, soil permeability, and hydrogeomorphic regions. Nutrient-input and watershed-characteristics datasets representing conditions during the late 1990s were merged with a connected network of stream reaches and watersheds to provide the spatial detail required by SPARROW. Stream-nutrient load estimates for 125 sampling sites (87 for total nitrogen and 103 for total phosphorus) served as the dependent variables for the regressions, and were used to calibrate models of total nitrogen and total phosphorus depicting late 1990s conditions in the Chesapeake Bay watershed. Spatial data generated for the models can be used to identify the location of nutrient sources, while the models' nutrient estimates can be used to evaluate stream-nutrient load contributed locally by each source evaluated, the amount of local load generated that is transported to the Bay, and the factors that affect the nutrient transport. Applying the SPARROW methodology to late 1990s information completes three time periods (late 1980s, early 1990s, and late 1990s) of viable data that resource managers can use to evaluate the water-quality conditions within the Bay watershed in order to refine restoration goals and nutrient-reduction strategies.

  6. High-resolution monitoring of catchment nutrient response to the end of the 2011-2012 drought in England, captured by the demonstration test catchments

    NASA Astrophysics Data System (ADS)

    Outram, F. N.; Lloyd, C.; Jonczyk, J.; Benskin, C. McW. H.; Grant, F.; Dorling, S. R.; Steele, C. J.; Collins, A. L.; Freer, J.; Haygarth, P. M.; Hiscock, K. M.; Johnes, P. J.; Lovett, A. L.

    2013-12-01

    The Demonstration Test Catchments (DTC) project is a UK Government funded initiative to test the effectiveness of on-farm mitigation measures designed to reduce agricultural pollution without compromising farm productivity. Three distinct catchments in England have been chosen to test the efficacy of mitigation measures on working farms in small tributary sub-catchments equipped with continuous water quality monitoring stations. The Hampshire Avon in the south is a mixed livestock and arable farming catchment, the River Wensum in the east is a lowland catchment with predominantly arable farming and land use in the River Eden catchment in the north-west is predominantly livestock farming. One of the many strengths of the DTC as a national research platform is that it provides the ability to investigate catchment hydrology and biogeochemical response across different landscapes and geoclimatic characteristics, with a range of differing flow behaviours, geochemistries and nutrient chemistries. Although numerous authors present studies of individual catchment responses to storms, no studies exist of multiple catchment responses to the same rainfall event captured with in situ high-resolution nutrient monitoring at a national scale. This paper brings together findings from all three DTC research groups to compare the response of the catchments to a major storm event in April 2012. This was one of the first weather fronts to track across the country following a prolonged drought period affecting much of the UK through 2011-2012, marking an unusual meteorological transition when a rapid shift from drought to flood risk occurred. The effects of the weather front on discharge and water chemistry parameters, including nitrogen species (NO3-N and NH4-N) and phosphorus fractions (total P (TP) and total reactive P (TRP)), measured at a half-hourly time step are examined. When considered in the context of one hydrological year, flow and concentration duration curves reveal that the weather fronts resulted in extreme flow, nitrate and TP concentrations in all three catchments but with distinct differences in both hydrographs and chemographs. Hysteresis loops constructed from high resolution data are used to highlight an array of potential pollutant sources and delivery pathways. In the Hampshire Avon DTC, transport was dominated by sub-surface processes, where phosphorus, largely in the soluble form, was found to be transport-limited. In the Wensum DTC, transport was largely dominated by rapid sub-surface movement due to the presence of under-drainage, which mobilised large quantities of nitrate during the storm. In the Eden DTC, transport was found to be initially dominated by surface runoff, which switched to subsurface delivery on the falling limb of the hydrograph, with the surface delivery transporting large amounts of particulate phosphorus to the river, with a transport-limited response. The lack of exhaustion of nutrient delivery in response to such extreme flow generation indicates the size of the nutrient pools stored in these catchments, and highlights the scale of the challenges faced by environmental managers when designing mitigation measures to reduce the flux of nutrients to UK river systems from diffuse agricultural sources.

  7. Factors Affecting Nitrate Delivery to Streams from Shallow Ground Water in the North Carolina Coastal Plain

    USGS Publications Warehouse

    Harden, Stephen L.; Spruill, Timothy B.

    2008-01-01

    An analysis of data collected at five flow-path study sites between 1997 and 2006 was performed to identify the factors needed to formulate a comprehensive program, with a focus on nitrogen, for protecting ground water and surface water in the North Carolina Coastal Plain. Water-quality protection in the Coastal Plain requires the identification of factors that affect the transport of nutrients from recharge areas to streams through the shallow ground-water system. Some basins process or retain nitrogen more readily than others, and the factors that affect nitrogen processing and retention were the focus of this investigation to improve nutrient management in Coastal Plain streams and to reduce nutrient loads to coastal waters. Nitrate reduction in ground water was observed at all five flow-path study sites in the North Carolina Coastal Plain, although the extent of reduction at each site was influenced by various environmental, hydrogeologic, and geochemical factors. Denitrification was the most common factor responsible for decreases in nitrate along the ground-water flow paths. Specific factors, some of which affect denitrification rates, that appeared to influence ground-water nitrate concentrations along the flow paths or in the streams include soil drainage, presence or absence of riparian buffers, evapotranspiration, fertilizer use, ground-water recharge rates and residence times, aquifer properties, subsurface tile drainage, sources and amounts of organic matter, and hyporheic processes. The study data indicate that the nitrate-reducing capacity of the buffer zone combined with that of the hyporheic zone can substantially lower the amount of ground-water nitrate discharged to streams in agricultural settings of the North Carolina Coastal Plain. At the watershed scale, the effects of ground-water discharge on surface-water quality appear to be greatly influenced by streamflow conditions and the presence of extensive riparian vegetation. Streamflow statistics that reflect base flow and the general hydrologic dynamics of a stream are important in understanding nutrient transport from a watershed and may be useful indicators of watersheds that are likely to have higher yields of nutrients and water. Combining streamflow statistics with information on such factors as land use, soil drainage, extent of riparian vegetation, geochemical conditions, and subsurface tile drainage in the Coastal Plain can be useful in identifying watersheds that are most likely to export excessive nitrogen due to nonpoint-source loadings and watersheds that are effective in processing nitrogen.

  8. Nutrient balance affects foraging behaviour of a trap-building predator

    PubMed Central

    Mayntz, David; Toft, Søren; Vollrath, Fritz

    2009-01-01

    Predator foraging may be affected by previous prey capture, but it is unknown how nutrient balance affects foraging behaviour. Here, we use a trap-building predator to test whether nutrients from previous prey captures affect foraging behaviour. We fed orb-weaving spiders (Zygiella x-notata) prey flies of different nutrient composition and in different amounts during their first instar and measured the subsequent frequency of web building and aspects of web architecture. We found that both the likelihood of web building and the number of radii in the web were affected by prey nutrient composition while prey availability affected capture area and mesh height. Our results show that both the balance of nutrients in captured prey and the previous capture rate may affect future foraging behaviour of predators. PMID:19640870

  9. Nutrient supplementation may adversely affect maternal oral health--a randomised controlled trial in rural Malawi.

    PubMed

    Harjunmaa, Ulla; Järnstedt, Jorma; Dewey, Kathryn G; Ashorn, Ulla; Maleta, Kenneth; Vosti, Stephen A; Ashorn, Per

    2016-01-01

    Nutritional supplementation during pregnancy is increasingly recommended especially in low-resource settings, but its oral health impacts have not been studied. Our aim was to examine whether supplementation with multiple micronutrients (MMN) or small-quantity lipid-based nutrient supplements affects dental caries development or periodontal health in a rural Malawian population. The study was embedded in a controlled iLiNS-DYAD trial that enrolled 1391 pregnant women <20 gestation weeks. Women were provided with one daily iron-folic acid capsule (IFA), one capsule with 18 micronutrients (MMN) or one sachet of lipid-based nutrient supplements (LNS) containing protein, carbohydrates, essential fatty acids and 21 micronutrients. Oral examination of 1024 participants was conducted and panoramic X-ray taken within 6 weeks after delivery. The supplement groups were similar at baseline in average socio-economic, nutritional and health status. At the end of the intervention, the prevalence of caries was 56.7%, 69.1% and 63.3% (P = 0.004), and periodontitis 34.9%, 29.8% and 31.2% (P = 0.338) in the IFA, MMN and LNS groups, respectively. Compared with the IFA group, women in the MMN group had 0.60 (0.18-1.02) and in the LNS group 0.59 (0.17-1.01) higher mean number of caries lesions. In the absence of baseline oral health data, firm conclusions on causality cannot be drawn. However, although not confirmatory, the findings are consistent with a possibility that provision of MMN or LNS may have increased the caries incidence in this target population. Because of the potential public health impacts, further research on the association between gestational nutrient interventions and oral health in low-income settings is needed. © 2015 John Wiley & Sons Ltd.

  10. High Levels of Dietary Supplement Vitamins A, C and E are Absorbed in the Small Intestine and Protect Nutrient Transport Against Chronic Gamma Irradiation.

    PubMed

    Roche, Marjolaine; Neti, Prasad V S V; Kemp, Francis W; Azzam, Edouard I; Ferraris, Ronaldo P; Howell, Roger W

    2015-11-01

    We examined nutrient transport in the intestines of mice exposed to chronic low-LET 137Cs gamma rays. The mice were whole-body irradiated for 3 days at dose rates of 0, 0.13 and 0.20 Gy/h, for total dose delivery of 0, 9.6 or 14.4 Gy, respectively. The mice were fed either a control diet or a diet supplemented with high levels of vitamins A, C and E. Our results showed that nutrient transport was perturbed by the chronic irradiation conditions. However, no apparent alteration of the macroscopic intestinal structures of the small intestine were observed up to day 10 after initiating irradiation. Jejunal fructose uptake measured in vitro was strongly affected by the chronic irradiation, whereas uptake of proline, carnosine and the bile acid taurocholate in the ileum was less affected. D-glucose transport did not appear to be inhibited significantly by either 9.6 or 14.4 Gy exposure. In the 14.4 Gy irradiated groups, the diet supplemented with high levels of vitamins A, C and E increased intestinal transport of fructose compared to the control diet (day 10; t test, P = 0.032), which correlated with elevated levels of vitamins A, C and E in the plasma and jejunal enterocytes. Our earlier studies with mice exposed acutely to 137Cs gamma rays demonstrated significant protection for transport of fructose, glucose, proline and carnosine. Taken together, these results suggest that high levels of vitamins A, C and E dietary supplements help preserve intestinal nutrient transport when intestines are irradiated chronically or acutely with low-LET gamma rays.

  11. High Levels of Dietary Supplement Vitamins A, C and E are Absorbed in the Small Intestine and Protect Nutrient Transport Against Chronic Gamma Irradiation

    PubMed Central

    Azzam, Edouard I.; Ferraris, Ronaldo P.; Howell, Roger W.

    2015-01-01

    We examined nutrient transport in the intestines of mice exposed to chronic low-LET 137Cs gamma rays. The mice were whole-body irradiated for 3 days at dose rates of 0, 0.13 and 0.20 Gy/h, for total dose delivery of 0, 9.6 or 14.4 Gy, respectively. The mice were fed either a control diet or a diet supplemented with high levels of vitamins A, C and E. Our results showed that nutrient transport was perturbed by the chronic irradiation conditions. However, no apparent alteration of the macroscopic intestinal structures of the small intestine were observed up to day 10 after initiating irradiation. Jejunal fructose uptake measured in vitro was strongly affected by the chronic irradiation, whereas uptake of proline, carnosine and the bile acid taurocholate in the ileum was less affected. D-glucose transport did not appear to be inhibited significantly by either 9.6 or 14.4 Gy exposure. In the 14.4 Gy irradiated groups, the diet supplemented with high levels of vitamins A, C and E increased intestinal transport of fructose compared to the control diet (day 10; t test, P = 0.032), which correlated with elevated levels of vitamins A, C and E in the plasma and jejunal enterocytes. Our earlier studies with mice exposed acutely to 137Cs gamma rays demonstrated significant protection for transport of fructose, glucose, proline and carnosine. Taken together, these results suggest that high levels of vitamins A, C and E dietary supplements help preserve intestinal nutrient transport when intestines are irradiated chronically or acutely with low-LET gamma rays. PMID:26484399

  12. Developmental Strategy For Effective Sampling To Detect Possible Nutrient Fluxes In Oligotrophic Coastal Reef Waters In The Caribbean

    NASA Astrophysics Data System (ADS)

    Mendoza, W. G.; Corredor, J. E.; Ko, D.; Zika, R. G.; Mooers, C. N.

    2008-05-01

    The increasing effort to develop the coastal ocean observing system (COOS) in various institutions has gained momentum due to its high value to climate, environmental, economic, and health issues. The stress contributed by nutrients to the coral reef ecosystem is among many problems that are targeted to be resolved using this system. Traditional nutrient sampling has been inadequate to resolve issues on episodic nutrient fluxes in reef regions due to temporal and spatial variability. This paper illustrates sampling strategy using the COOS information to identify areas that need critical investigation. The area investigated is within the Puerto Rico subdomain (60-70oW, 15-20oN), and Caribbean Time Series (CaTS), World Ocean Circulation Experiment (WOCE), Intra-America Sea (IAS) ocean nowcast/forecast system (IASNFS), and other COOS-related online datasets are utilized. Nutrient profile results indicate nitrate is undetectable in the upper 50 m apparently due to high biological consumption. Nutrients are delivered in Puerto Rico particularly in the CaTS station either via a meridional jet formed from opposing cyclonic and anticyclonic eddies or wind-driven upwelling. The strong vertical fluctuation in the upper 50 m demonstrates a high anomaly in temperature and salinity and a strong cross correlation signal. High chlorophyll a concentration corresponding to seasonal high nutrient influx coincides with higher precipitation accumulation rates and apparent riverine input from the Amazon and Orinoco Rivers during summer (August) than during winter (February) seasons. Non-detectability of nutrients in the upper 50 m is a reflection of poor sampling frequency or the absence of a highly sensitive nutrient analysis method to capture episodic events. Thus, this paper was able to determine the range of depths and concentrations that need to be critically investigated to determine nutrient fluxes, nutrient sources, and climatological factors that can affect nutrient delivery. It also provides some insight into needed sampling rates and temporal and spatial domain choices. Finally, it demonstrates a scientific reconnaissance for a field study that is now possible with online in-situ and remote sensing observations and numerical simulations, as a consequence of IOOS in general and COOS in particular.

  13. WATER LEVEL AND OXYGEN DELIVERY/UTILIZATION IN POROUS SALT MARSH SEDIMENTS

    EPA Science Inventory

    Increasing terrestrial nutrient inputs to coastal waters is a global water quality issue worldwide, and salt marshes may provide a valuable nutrient buffer, either by direct removal or by smoothing out pulse inputs between sources and sensitive estuarine habitats. A major challen...

  14. Pancreatic enzyme replacement therapy.

    PubMed

    Layer, P; Keller, J; Lankisch, P G

    2001-04-01

    Malabsorption due to severe pancreatic exocrine insufficiency is one of the most important late features of chronic pancreatitis. Generally, steatorrhea is more severe and occurs several years prior to malabsorption of other nutrients because synthesis and secretion of lipase are impaired more rapidly, its intraluminal survival is shorter, and the lack of pancreatic lipase activity is not compensated for by nonpancreatic mechanisms. Patients suffer not only from nutritional deficiencies but also from increased nutrient delivery to distal intestinal sites, causing symptoms by profound alteration of upper gastrointestinal secretory and motor functions. Adequate nutrient absorption requires delivery of sufficient enzymatic activity into the duodenal lumen simultaneously with meal nutrients. The following recommendations are based on modern therapeutic concepts: 25,000 to 40,000 units of lipase per meal using pH-sensitive pancreatin microspheres, with dosage increases, compliance checks, and differential diagnosis in case of treatment failure. Still, in most patients, lipid digestion cannot be completely normalized by current standard therapy, and future developments are needed to optimize treatment.

  15. Interfacing carbon nanotubes (CNT) with plants: enhancement of growth, water and ionic nutrient uptake in maize ( Zea mays) and implications for nanoagriculture

    NASA Astrophysics Data System (ADS)

    Tiwari, D. K.; Dasgupta-Schubert, N.; Villaseñor Cendejas, L. M.; Villegas, J.; Carreto Montoya, L.; Borjas García, S. E.

    2014-06-01

    The application of nano-biotechnology to crop-science/agriculture (`nanoagriculture') is a recent development. While carbon nanotubes (CNTs) have been shown to dramatically improve germination of some comestible plants, deficiencies in consistency of behavior and reproducibility arise, partially from the variability of the CNTs used. In this work, factory-synthesized multi-walled-CNTs (MWCNTs) of quality-controlled specifications were seen to enhance the germinative growth of maize seedlings at low concentrations but depress it at higher concentrations. Growth enhancement principally arose through improved water delivery by the MWCNT. Polarized EDXRF spectrometry showed that MWCNTs affect mineral nutrient supply to the seedling through the action of the mutually opposing forces of inflow with water and retention in the medium by the ion-CNT transient-dipole interaction. The effect varied with ion type and MWCNT concentration. The differences of the Fe tissue concentrations when relatively high equimolar Fe2+ or Fe3+ was introduced, implied that the ion-CNT interaction might induce redox changes to the ion. The tissue Ca2+ concentration manifested as the antipode of the Fe2+ concentration indicating a possible cationic exchange in the cell wall matrix. SEM images showed that MWCNTs perforated the black-layer seed-coat that could explain the enhanced water delivery. The absence of perforations with the introduction of FeCl2/FeCl3 reinforces the idea of the modification of MWCNT functionality by the ion-CNT interaction. Overall, in normal media, low dose MWCNTs were seen to be beneficial, improving water absorption, plant biomass and the concentrations of the essential Ca, Fe nutrients, opening a potential for possible future commercial agricultural applications.

  16. Dissolved Nutrient Retention Dynamics in River Networks: A Modeling Investigation of Transient Flow and Scale Effects

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ye, Sheng; Covino, Timothy P.; Sivapalan, Murugesu

    In this paper, we use a dynamic network flow model, coupled with a transient storage zone biogeochemical model, to simulate dissolved nutrient removal processes at the channel network scale. We have explored several scenarios in respect of the combination of rainfall variability, and the biological and geomorphic characteristics of the catchment, to understand the dominant controls on removal and delivery of dissolved nutrients (e.g., nitrate). These model-based theoretical analyses suggested that while nutrient removal efficiency is lower during flood events compared to during baseflow periods, flood events contribute significantly to bulk nutrient removal, whereas bulk removal during baseflow periods ismore » less. This is due to the fact that nutrient supply is larger during flood events; this trend is even stronger in large rivers. However, the efficiency of removal during both periods decreases in larger rivers, however, due to (i) increasing flow velocities and thus decreasing residence time, and (ii) increasing flow depth, and thus decreasing nutrient uptake rates. Besides nutrient removal processes can be divided into two parts: in the main channel and in the hyporheic transient storage zone. When assessing their relative contributions the size of the transient storage zone is a dominant control, followed by uptake rates in the main channel and in the transient storage zone. Increasing size of the transient storage zone with downstream distance affects the relative contributions to nutrient removal of the water column and the transient storage zone, which also impacts the way nutrient removal rates scale with increasing size of rivers. Intra-annual hydrologic variability has a significant impact on removal rates at all scales: the more variable the streamflow is, compared to mean discharge, the less nutrient is removed in the channel network. A scale-independent first order uptake coefficient, ke, estimated from model simulations, is highly dependent on the relative size of the transient storage zone and how it changes in the downstream direction, as well as the nature of hydrologic variability.« less

  17. Nutrient delivery from the Mississippi River to the Gulf of Mexico and effects of cropland conservation

    USDA-ARS?s Scientific Manuscript database

    Excessive nutrients transported from the Mississippi River Basin have created an ecological disaster - Gulf of Mexico hypoxia. Also, in recent years, federal expenditures on agricultural conservation practices have received intense scrutiny. Partly driven by these factors, the USDA Conservation Ef...

  18. Sildenafil citrate treatment enhances amino acid availability in the conceptus and fetal growth in an ovine model of intrauterine growth restriction.

    PubMed

    Satterfield, M Carey; Bazer, Fuller W; Spencer, Thomas E; Wu, Guoyao

    2010-02-01

    Adequate placental blood flow is essential for the optimal delivery of nutrients from mother to fetus for conceptus growth. Restricted fetal development results from pathophysiological and environmental factors that alter utero-placental blood flow, placental function, and, therefore, nutrient availability in the fetus. To test this hypothesis, 0, 75, or 150 mg/d sildenafil citrate (Viagra) was administered subcutaneously from d 28 to 115 of gestation to either nutrient-restricted [50% of NRC requirements) or adequately-fed ewes (100% of NRC requirements). On d 115, maternal, fetal, and placental tissues and fluids were collected. Concentrations of total amino acids and polyamines in uterine venous and arterial sera, amniotic and allantoic fluids, and fetal umbilical venous serum were lower (P < 0.05) in nutrient-restricted ewes than in adequately fed ewes, as were the ratios of total amino acids in fetal umbilical venous serum to uterine arterial serum. Sildenafil citrate dose-dependently increased (P < 0.05) total amino acids and polyamines in amniotic fluid, allantoic fluid, and fetal serum without affecting values in maternal serum. Fetal weight was lower (P < 0.05) in nutrient-restricted ewes on d 115. Sildenafil citrate treatment dose-dependently increased (P < 0.05) fetal weight in both nutrient-restricted and adequately fed ewes. This study supports the hypothesis that long-term sildenafil citrate treatment enhances fetal growth, at least in part, by increasing the availability of amino acids in the conceptus. These findings may lead to the clinical use of sildenafil citrate in human pregnancies suspected to be at risk for intrauterine fetal growth retardation.

  19. Prenatal Lipid-Based Nutrient Supplements Do Not Affect Pregnancy or Childbirth Complications or Cesarean Delivery in Bangladesh: A Cluster-Randomized Controlled Effectiveness Trial.

    PubMed

    Mridha, Malay K; Matias, Susana L; Paul, Rina Rani; Hussain, Sohrab; Sarker, Mostofa; Hossain, Mokbul; Peerson, Janet M; Vosti, Stephen A; Dewey, Kathryn G

    2017-09-01

    Background: Pregnancy and childbirth complications and cesarean delivery are common in Bangladesh. Objective: We evaluated the effect of lipid-based nutrient supplements for pregnant and lactating women (LNS-PL) on pregnancy and childbirth complications and cesarean delivery. Methods: We conducted the Rang-Din Nutrition Study, a cluster-randomized controlled effectiveness trial within a community health program in rural Bangladesh. We enrolled 4011 pregnant women in early pregnancy. Women in 48 clusters received iron and folic acid (IFA; 60 mg Fe + 400 μg folic acid/d) and women in 16 clusters received LNS-PL (20 g/d, 118 kcal) containing essential fatty acids and 22 vitamins and minerals. Pregnancy and childbirth complications and the cesarean delivery rate were secondary outcomes of the study. Results: Women in the LNS-PL group did not differ significantly from the IFA group with respect to mean systolic blood pressure at 36 wk gestation (113 and 112 mm Hg; P = 0.17), diastolic blood pressure at 36 wk gestation (68.9 and 68.7 mmHg; P = 0.88), or mean total number of pregnancy and childbirth complications (0.32 and 0.31; P = 0.86). They also did not differ significantly with respect to the prevalence of high blood pressure at 36 wk (1.74% and 2.03%; P = 0.62), antepartum hemorrhage (0.83% and 1.39%; P = 0.21), prolonged labor (8.34% and 8.79%; P = 0.68), early rupture of membranes (9.30% and 8.45%; P = 0.43), convulsions (1.57% and 1.08%; P = 0.24), high blood pressure in labor (1.54% and 1.19%; P = 0.46), obstructed labor (2.83% and 2.91%; P = 0.90), any complications during pregnancy or childbirth (35.9% and 37.1%; P = 0.64), episiotomy (6.31% and 6.44%; P = 0.90), or cesarean delivery (15.6% and 14.2%; P = 0.48). Conclusion: Compared with IFA, antenatal LNS-PL did not increase or decrease pregnancy and childbirth complications or cesarean delivery among women in rural Bangladesh. This trial was registered at clinicaltrials.gov as NCT01715038. © 2017 American Society for Nutrition.

  20. Global riverine N and P transport to ocean increased during the 20th century despite increased retention along the aquatic continuum

    NASA Astrophysics Data System (ADS)

    Beusen, Arthur H. W.; Bouwman, Alexander F.; Van Beek, Ludovicus P. H.; Mogollón, José M.; Middelburg, Jack J.

    2016-04-01

    Various human activities - including agriculture, water consumption, river damming, and aquaculture - have intensified over the last century. This has had a major impact on nitrogen (N) and phosphorus (P) cycling in global continental waters. In this study, we use a coupled nutrient-input-hydrology-in-stream nutrient retention model to quantitatively track the changes in the global freshwater N and P cycles over the 20th century. Our results suggest that, during this period, the global nutrient delivery to streams increased from 34 to 64 Tg N yr-1 and from 5 to 9 Tg P yr-1. Furthermore, in-stream retention and removal grew from 14 to 27 Tg N yr-1 and 3 to 5 Tg P yr-1. One of the major causes of increased retention is the growing number of reservoirs, which now account for 24 and 22 % of global N and P retention/removal in freshwater systems, respectively. This increase in nutrient retention could not balance the increase in nutrient delivery to rivers with the consequence that river nutrient transport to the ocean increased from 19 to 37 Tg N yr-1 and from 2 to 4 Tg P yr-1. Human activities have also led to a global increase in the molar N : P ratio in freshwater bodies.

  1. Global riverine N and P transport to ocean increased during the twentieth century despite increased retention along the aquatic continuum

    NASA Astrophysics Data System (ADS)

    Beusen, A. H. W.; Bouwman, A. F.; Van Beek, L. P. H.; Mogollón, J. M.; Middelburg, J. J.

    2015-12-01

    Various human activities, including agriculture, water consumption, river damming, and aquaculture, have intensified over the last century. This has had a major impact on nitrogen (N) and phosphorus (P) cycling in global continental waters. In this study, we use a coupled nutrient-input, hydrology, in-stream nutrient retention model to quantitatively track the changes in the global freshwater N and P cycles over the 20th century. Our results suggest that, during this period, the global nutrient delivery to streams increased from 34 to 64 Tg N yr-1 and from 5 to 9 Tg N yr-1. Furthermore, in-stream retention and removal grew from 14 to 27 Tg N yr-1 and 3 to 5 Tg N yr-1. One of the major cause of increased retention is the growing number of reservoirs which now account for 24 and 22 % of global N and P retention/removal in freshwater systems, respectively. This increase in nutrient retention could not balance the increase in nutrient delivery to rivers with the consequence that river nutrient transport to the ocean increased from 19 to 37 Tg N yr-1 and from 2 to 4 Tg N yr-1. Human activities have also led to a global increase in the molar N : P ratio in freshwater bodies.

  2. Novel starch based nano scale enteric coatings from soybean meal for colon-specific delivery

    USDA-ARS?s Scientific Manuscript database

    Soybean meal was used to isolate resistant starch and produce nanoparticles, which could be potential coating materials for colonic nutrient and drug deliveries. The nanoparticles were in 40 +/- 33.2 nm ranges. These nanoparticles were stable under simulated human physiological conditions. The deg...

  3. A System for Managing Replenishment of a Nutrient Solution Using an Electrical Conductivity Controller

    NASA Technical Reports Server (NTRS)

    Davis, D.; Dogan, N.; Aglan, H.; Mortley, D.; Loretan, P.

    1998-01-01

    Control of nutrient solution parameters is very important for the growth and development of plants grown hydroponically. Protocols involving different nutrient solution replenishment times (e.g. one-week, two-week, or two-day replenishment) provide manual periodic control of the nutrient solution's electrical conductivity (EC). Since plants take-up nutrients as they grow, manual control has a drawback in that EC is not held constant between replenishments. In an effort to correct this problem the Center for Food and Environmental Systems for Human Exploration of Space at Tuskegee University has developed a system for managing and controlling levels of EC over a plant's entire growing cycle. A prototype system is being tested on sweetpotato production using the nutrient film technique (NFT), and it is being compared to a system in which sweetpotatoes are grown using NFT with manual control. NASA has played an important role in the development of environmental control systems. They have become a forerunner in growing plants hydroponically with some control systems through the use of networked data acquisition and control using environmental growth chambers. Data acquisition systems which involve the use of real-time, calibration, set points, user panel, and graphical representation programming provide a good method of controlling nutrient solution parameters such as EC and pH [Bledsoe, 19931]. In NASA's Biomass Production Chamber (BPC) at Kennedy Space Center, control is provided by a programmable logic controller (PLC). This is an industrial controller which combines ladder computer logic which has the ability to handle various levels of electrical power. The controller controls temperature, light and other parameters that affect the plant's environment, in the BPC, the Nutrient Delivery System (NIX), a sub-system of the PLC, controls nutrient solution parameters such as EC, pH, and solution levels. When the nutrient EC measurement goes outside a preset range (120-130 mS/m) a set amount of a stock solution of nutrients is automatically added by a metering pump to bring the EC back into operating range [Fortson, 1992]. This paper describes a system developed at Tuskegee University for controlling the EC of a nutrient solution used for growing sweetpotatoes with an EC controller and a computer with LabView data acquisition and instrumentation software. It also describes the preliminary data obtained from the growth of sweetpotatoes using this prototype control system.

  4. Reducing fertilizer-nitrogen losses from rowcrop landscapes: Insights and implications from a spatially explicit watershed model

    USGS Publications Warehouse

    McLellan, Eileen; Schilling, Keith; Robertson, Dale M.

    2015-01-01

    We present conceptual and quantitative models that predict changes in fertilizer-derived nitrogen delivery from rowcrop landscapes caused by agricultural conservation efforts implemented to reduce nutrient inputs and transport and increase nutrient retention in the landscape. To evaluate the relative importance of changes in the sources, transport, and sinks of fertilizer-derived nitrogen across a region, we use the spatially explicit SPAtially Referenced Regression On Watershed attributes watershed model to map the distribution, at the small watershed scale within the Upper Mississippi-Ohio River Basin (UMORB), of: (1) fertilizer inputs; (2) nutrient attenuation during delivery of those inputs to the UMORB outlet; and (3) nitrogen export from the UMORB outlet. Comparing these spatial distributions suggests that the amount of fertilizer input and degree of nutrient attenuation are both important in determining the extent of nitrogen export. From a management perspective, this means that agricultural conservation efforts to reduce nitrogen export would benefit by: (1) expanding their focus to include activities that restore and enhance nutrient processing in these highly altered landscapes; and (2) targeting specific types of best management practices to watersheds where they will be most valuable. Doing so successfully may result in a shift in current approaches to conservation planning, outreach, and funding.

  5. Designing food delivery systems: challenges related to the in vitro methods employed to determine the fate of bioactives in the gut.

    PubMed

    Arranz, Elena; Corredig, Milena; Guri, Anilda

    2016-08-10

    An in depth understanding of the underpinning mechanisms that relate to food disruption and processing in the gastrointestinal tract is necessary to achieve optimal intake of nutrients and their bioefficacy. Although in vivo trials can provide insights on physiological responses of nutrients, in vitro assays are often applied as tools to understand specific mechanisms, or as prescreening methods to determine the factors associated with the uptake of food components in the gastrointestinal tract. In vitro assays are also often utilized to design novel or improved food delivery systems. In this review the available approaches to study delivery and uptake of food bioactives and the associated challenges are discussed. For an in depth understanding of food processing in the gastrointestinal tract, it is necessary to apply multidisciplinary methodologies, at the interface between materials science, chemistry, physics and biology.

  6. Premature Brain Aging in Baboons Resulting from Moderate Fetal Undernutrition.

    PubMed

    Franke, Katja; Clarke, Geoffrey D; Dahnke, Robert; Gaser, Christian; Kuo, Anderson H; Li, Cun; Schwab, Matthias; Nathanielsz, Peter W

    2017-01-01

    Contrary to the known benefits from a moderate dietary reduction during adulthood on life span and health, maternal nutrient reduction during pregnancy is supposed to affect the developing brain, probably resulting in impaired brain structure and function throughout life. Decreased fetal nutrition delivery is widespread in both developing and developed countries, caused by poverty and natural disasters, but also due to maternal dieting, teenage pregnancy, pregnancy in women over 35 years of age, placental insufficiency, or multiples. Compromised development of fetal cerebral structures was already shown in our baboon model of moderate maternal nutrient reduction. The present study was designed to follow-up and evaluate the effects of moderate maternal nutrient reduction on individual brain aging in the baboon during young adulthood (4-7 years; human equivalent 14-24 years), applying a novel, non-invasive neuroimaging aging biomarker. The study reveals premature brain aging of +2.7 years ( p < 0.01) in the female baboon exposed to fetal undernutrition. The effects of moderate maternal nutrient reduction on individual brain aging occurred in the absence of fetal growth restriction or marked maternal weight reduction at birth, which stresses the significance of early nutritional conditions in life-long developmental programming. This non-invasive MRI biomarker allows further longitudinal in vivo tracking of individual brain aging trajectories to assess the life-long effects of developmental and environmental influences in programming paradigms, aiding preventive and curative treatments on cerebral atrophy in experimental animal models and humans.

  7. Premature Brain Aging in Baboons Resulting from Moderate Fetal Undernutrition

    PubMed Central

    Franke, Katja; Clarke, Geoffrey D.; Dahnke, Robert; Gaser, Christian; Kuo, Anderson H.; Li, Cun; Schwab, Matthias; Nathanielsz, Peter W.

    2017-01-01

    Contrary to the known benefits from a moderate dietary reduction during adulthood on life span and health, maternal nutrient reduction during pregnancy is supposed to affect the developing brain, probably resulting in impaired brain structure and function throughout life. Decreased fetal nutrition delivery is widespread in both developing and developed countries, caused by poverty and natural disasters, but also due to maternal dieting, teenage pregnancy, pregnancy in women over 35 years of age, placental insufficiency, or multiples. Compromised development of fetal cerebral structures was already shown in our baboon model of moderate maternal nutrient reduction. The present study was designed to follow-up and evaluate the effects of moderate maternal nutrient reduction on individual brain aging in the baboon during young adulthood (4–7 years; human equivalent 14–24 years), applying a novel, non-invasive neuroimaging aging biomarker. The study reveals premature brain aging of +2.7 years (p < 0.01) in the female baboon exposed to fetal undernutrition. The effects of moderate maternal nutrient reduction on individual brain aging occurred in the absence of fetal growth restriction or marked maternal weight reduction at birth, which stresses the significance of early nutritional conditions in life-long developmental programming. This non-invasive MRI biomarker allows further longitudinal in vivo tracking of individual brain aging trajectories to assess the life-long effects of developmental and environmental influences in programming paradigms, aiding preventive and curative treatments on cerebral atrophy in experimental animal models and humans. PMID:28443017

  8. Synthesis and evaluation of a superabsorbent-fertilizer composite for maximizing the nutrient and water use efficiency in forestry plantations.

    PubMed

    Tubert, E; Vitali, V A; Alvarez, M S; Tubert, F A; Baroli, I; Amodeo, G

    2018-03-15

    Reducing fertilizer use is a priority in the quest for sustainable forestry systems. In short rotation Eucalyptus plantations, NPK pellets are routinely added to the seedling's top soil layer at planting, potentially leading to increased seedling mortality, nutrient loss and environmental degradation. To address this triple challenge, the development of efficient fertilization practices is essential. In the present work, we synthesized a crosslinked acrylic-cellulosic superabsorbent composite (SAPH-BAL) containing small amounts of specific nutrients integrated in the polymer matrix. We analyzed the composite's chemical and rheological properties, and assessed the viability of Eucalyptus plantations supplied with it at planting. Physiological measurements confirmed the suitability of SAPH-BAL in greenhouse-grown potted seedlings subjected to different growth conditions, showing that it efficiently delivers nutrients while protecting seedlings from drought stress. Field experiments carried out at ten South American locations covering an ample range of environmental conditions confirmed the beneficial effect of SAPH-BAL on growth and survival in comparison to the conventional fertilization scheme (superabsorbent + 75 g NPK). Furthermore, it was found that plants treated with SAPH-BAL were less affected by the differences in rainfall regimes during the experiments compared to those fertilized conventionally. To the best of our knowledge this is the first report describing the successful use of superabsorbents for root targeted delivery of fertilizers in forestry operations. Copyright © 2018 Elsevier Ltd. All rights reserved.

  9. Mucus interactions with liposomes encapsulating bioactives: Interfacial tensiometry and cellular uptake on Caco-2 and cocultures of Caco-2/HT29-MTX.

    PubMed

    Li, Yang; Arranz, Elena; Guri, Anilda; Corredig, Milena

    2017-02-01

    Structuring of delivery matrices in foods aquires careful designing for optimal delivery and subsiquent absorption of the beneficial compounds in the gut. There has been quite improvement in mimicking digestion and absorption in vitro but as of yet little is understood on mucus interference in nutrient absorption Therefore in this study interactions of human intestinal mucus with milk and soy phospholipids liposomes carring hydrophilic (epigallocatechin-3-gallate) or hydrophobic (β-carotene) bioactive molecules were investigated. Liposomes of about 100nm were obtained using microfluidization and their behaviour with the human intestinal mucus were evaluated using drop shape tensiometry. The chemistry of the liposomes (milk or soy) and the encapsulated bioactive structure can affect the viscoelastic behaviour of the complex itself. Empty or loaded liposomes were differently interacting with the mucus at the interface. Mucus-liposomes interactions were also studied using cell cultures, Caco-2 (without mucus) and cocultures Caco-2/HT29-MTX (mucus producing). The interaction of mucus layer with liposomes was at some extent aligned with rheological studies. This work demonstrated that delivery systems may interact with the mucosal surface of intestinal cells, and in vitro approaches allow for screening of such interactions. These highlights could help us in carefully designing the delivery systems and moreover choosing the right carrier and/or bioactive that does not jeopardize the optimal delivery of the bioactive structure. Copyright © 2016 Elsevier Ltd. All rights reserved.

  10. Exploring the impact of agriculture on nitrogen and phosphorus biogeochemistry in global rivers during the twentieth century (Invited)

    NASA Astrophysics Data System (ADS)

    Bouwman, L.; Beusen, A.; Van Beek, L. P.

    2013-12-01

    Nutrients are transported from land to sea through the continuum formed by soils, groundwater, riparian zones, floodplains, streams, rivers, lakes, and reservoirs. The hydrology, ecology and biogeochemical processing in each of these components are strongly coupled and result in retention of a significant fraction of the nutrients transported. This paper analyzes the global changes in nutrient biogeochemical processes and retention in rivers during the past century (1900-2000); this period encompasses dramatic increases in human population and economic human activities including agriculture that have resulted in major changes in land use, nutrient use in agriculture, wastewater flows and human interventions in the hydrology (1). We use the hydrological PCR-GLOBWB model (2) for the period 1900-2000, including climate variability and the history of dam construction and land use conversion. Global agricultural and natural N and P soil budgets for the period 1900-2000 are the starting point to simulate nutrient flows from the soil via surface runoff and leaching through the groundwater system and riparian zones. In-stream processes are described with the nutrient spiraling concept. In the period 1900-2000, the global soil N budget surplus (inputs minus withdrawal in harvested crops) for agricultural and natural ecosystems increased from 118 to 202 Tg yr-1, and the global P budget increased from < 0.5 to 11 Tg P yr-1. As a result of this massive increase, nutrient delivery to streams and river nutrient export has increased rapidly in the 20th century. Model results are sensitive to factors determining the N and P delivery, as well as in-stream processes. The most uncertain factors are N delivery to streams by groundwater (denitrification as a function of thickness and reactivity of aquifers), and in-stream N and P retention parameters (net uptake velocity, retention as function of concentration). References 1. Bouwman AF, Beusen AHW, Griffioen J, Van Groenigen JW, Hefting MM, Oenema O, et al. Global trends and uncertainties in terrestrial denitrification and N2O emissions. Philosophical Transactions of the Royal Society B: Biological Sciences. 2013;368(20130112). 2. Van Beek LPH, Wada Y, Bierkens MFP. Global monthly water stress: 1. Water balance and water availability. Water Resour Res. 2011;47(7):W07517.

  11. Providing lipid-based nutrient supplement during pregnancy does not reduce the risk of maternal P falciparum parasitaemia and reproductive tract infections: a randomised controlled trial.

    PubMed

    Nkhoma, Minyanga; Ashorn, Per; Ashorn, Ulla; Dewey, Kathryn G; Gondwe, Austrida; Mbotwa, John; Rogerson, Stephen; Taylor, Steve M; Maleta, Kenneth

    2017-01-17

    Maternal infections are associated with maternal and foetal adverse outcomes. Nutrient supplementation during pregnancy may reduce the occurrence of infections by improving maternal immunity. We aimed to investigate the impact of small-quantity lipid-based nutrient supplement (SQ-LNS) on the occurrence of Plasmodium falciparum parasitaemia during pregnancy and trichomoniasis, vaginal candidiasis and urinary tract infection (UTI) after delivery. Pregnant Malawian women enrolled in the iLiNS-DYAD trial receiving daily supplementation with SQ-LNS, multiple micronutrients (MMN) or iron & folic acid (IFA) from <20 gestation weeks (gw) were assessed for P. falciparum parasitaemia at 32 gw using rapid diagnostic testing (RDT), at 36 gw using polymerase chain reaction (PCR) and at delivery using both RDT and PCR; and at one week after delivery for trichomoniasis and vaginal candidiasis using wet mount microscopy and for UTI using urine dipstick analysis. The prevalence of each infection by intervention group was estimated at the prescribed time points and the global null hypothesis was tested using logistic regression. Adjusted analyses were performed using preselected covariates. The prevalence of P. falciparum parasitaemia was 10.7% at 32 gw, 9% at 36 gw, and 8.3% by RDT and 20.2% by PCR at delivery. After delivery the prevalence of trichomoniasis was 10.5%, vaginal candidiasis was 0.5%, and UTI was 3.1%. There were no differences between intervention groups in the prevalence of any of the infections. In this population, SQ-LNS did not influence the occurrence of maternal P. falciparum parasitaemia, trichomoniasis, vaginal candidiasis or UTI. Identifier: NCT01239693 (10 November 2010).

  12. Gestational intake of methyl donors and global LINE-1 DNA methylation in maternal and cord blood: prospective results from a folate-replete population.

    PubMed

    Boeke, Caroline E; Baccarelli, Andrea; Kleinman, Ken P; Burris, Heather H; Litonjua, Augusto A; Rifas-Shiman, Sheryl L; Tarantini, Letizia; Gillman, Matthew

    2012-03-01

    Maternal diet affects offspring DNA methylation in animal models, but evidence from humans is limited. We investigated the extent to which gestational intake of methyl donor nutrients affects global DNA methylation in maternal and umbilical cord blood. Among mother-infant pairs in Project Viva, a folate-replete US population, we estimated maternal intakes of vitamin B12, betaine, choline, folate, cadmium, zinc and iron periconceptionally and during the second trimester. We examined associations of these nutrients with DNA methylation, measured as %5-methyl cytosines (%5mC) in Long Interspersed Nuclear Element-1 (LINE-1), in first trimester (n = 830) and second trimester (n = 671) maternal blood and in cord blood at delivery (n = 516). Cord blood methylation was higher for male than female infants {mean [standard deviation (SD)] 84.8 [0.6] vs. 84.4 [0.7]%}. In the multivariable-adjusted model, maternal intake of methyl donor nutrients periconceptionally and during the second trimester of pregnancy was not positively associated with first trimester, second trimester or cord blood LINE-1 methylation. Periconceptional betaine intake was inversely associated with cord blood methylation [regression coefficient = -0.08% (95% confidence interval (CI): -0.14,-0.01)] but this association was attenuated after adjustment for dietary cadmium, which itself was directly associated with first trimester methylation and inversely associated with cord blood methylation. We also found an inverse association between periconceptional choline [-0.10%, 95% CI: -0.17,-0.03 for each SD (~63 mg/day)] and cord blood methylation in males only. In this folate-replete population, we did not find positive associations between intake of methyl donor nutrients during pregnancy and DNA methylation overall, but among males, higher early pregnancy intakes of choline were associated with lower cord blood methylation.

  13. Gestational intake of methyl donors and global LINE-1 DNA methylation in maternal and cord blood

    PubMed Central

    Baccarelli, Andrea; Kleinman, Ken P; Burris, Heather H; Litonjua, Augusto A; Rifas-Shiman, Sheryl L; Tarantini, Letizia; Gillman, Matthew W

    2012-01-01

    Maternal diet affects offspring DNA methylation in animal models, but evidence from humans is limited. We investigated the extent to which gestational intake of methyl donor nutrients affects global DNA methylation in maternal and umbilical cord blood. Among mother-infant pairs in Project Viva, a folate-replete US population, we estimated maternal intakes of vitamin B12, betaine, choline, folate, cadmium, zinc and iron periconceptionally and during the second trimester. We examined associations of these nutrients with DNA methylation, measured as %5-methyl cytosines (%5mC) in Long Interspersed Nuclear Element-1 (LINE-1), in first trimester (n = 830) and second trimester (n = 671) maternal blood and in cord blood at delivery (n = 516). Cord blood methylation was higher for male than female infants {mean [standard deviation (SD)] 84.8 [0.6] vs. 84.4 [0.7]%}. In the multivariable-adjusted model, maternal intake of methyl donor nutrients periconceptionally and during the second trimester of pregnancy was not positively associated with first trimester, second trimester or cord blood LINE-1 methylation. Periconceptional betaine intake was inversely associated with cord blood methylation [regression coefficient = −0.08% (95% confidence interval (CI): −0.14, −0.01)] but this association was attenuated after adjustment for dietary cadmium, which itself was directly associated with first trimester methylation and inversely associated with cord blood methylation. We also found an inverse association between periconceptional choline [−0.10%, 95% CI: −0.17, −0.03 for each SD (∼63 mg/day)] and cord blood methylation in males only. In this folate-replete population, we did not find positive associations between intake of methyl donor nutrients during pregnancy and DNA methylation overall, but among males, higher early pregnancy intakes of choline were associated with lower cord blood methylation. PMID:22430801

  14. Adaptive Management of Return Flows: Lessons from a Case Study in Environmental Water Delivery to a Floodplain River

    NASA Astrophysics Data System (ADS)

    Wolfenden, Benjamin J.; Wassens, Skye M.; Jenkins, Kim M.; Baldwin, Darren S.; Kobayashi, Tsuyoshi; Maguire, James

    2018-03-01

    For many floodplain rivers, reinstating wetland connectivity is necessary for ecosystems to recover from decades of regulation. Environmental return flows (the managed delivery of wetland water to an adjacent river) can be used strategically to facilitate natural ecosystem connectivity, enabling the transfer of nutrients, energy, and biota from wetland habitats to the river. Using an informal adaptive management framework, we delivered return flows from a forested wetland complex into a large lowland river in south-eastern Australia. We hypothesized that return flows would (a) increase river nutrient concentrations; (b) reduce wetland nutrient concentrations; (c) increase rates of ecosystem metabolism through the addition of potentially limiting nutrients, causing related increases in the concentration of water column chlorophyll-a; and (d) increase the density and species richness of microinvertebrates in riverine benthic habitats. Our monitoring results demonstrated a small increase in the concentrations of several key nutrients but no evidence for significant ecological responses was found. Although return flows can be delivered from forested floodplain areas without risking hypoxic blackwater events, returning nutrient and carbon-rich water to increase riverine productivity is limited by the achievable scale of return flows. Nevertheless, using return flows to flush carbon from floodplains may be a useful management tool to reduce carbon loads, preparing floodplains for subsequent releases (e.g., mitigating the risk of hypoxic blackwater events). In this example, adaptive management benefited from a semi-formal collaboration between science and management that allowed for prompt decision-making.

  15. Membrane Cholesterol in Skeletal Muscle: A Novel Player in Excitation-Contraction Coupling and Insulin Resistance

    PubMed Central

    Barrientos, G.; Sánchez-Aguilera, P.; Jaimovich, E.; Hidalgo, C.

    2017-01-01

    Membrane cholesterol is critical for signaling processes in a variety of tissues. We will address here current evidence supporting an emerging role of cholesterol on excitation-contraction coupling and glucose transport in skeletal muscle. We have centered our review on the transverse tubule system, a complex network of narrow plasma membrane invaginations that propagate membrane depolarization into the fiber interior and allow nutrient delivery into the fibers. We will discuss current evidence showing that transverse tubule membranes have remarkably high cholesterol levels and we will address how modifications of cholesterol content influence excitation-contraction coupling. In addition, we will discuss how membrane cholesterol levels affect glucose transport by modulating the insertion into the membrane of the main insulin-sensitive glucose transporter GLUT4. Finally, we will address how the increased membrane cholesterol levels displayed by obese animals, which also present insulin resistance, affect these two particular skeletal muscle functions. PMID:28367451

  16. Transient osteoporosis of pregnancy.

    PubMed

    Maliha, George; Morgan, Jordan; Vrahas, Mark

    2012-08-01

    Transient osteoporosis of pregnancy (TOP) is a rare yet perhaps under-reported condition that has affected otherwise healthy pregnancies throughout the world. The condition presents suddenly in the third trimester of a usually uneventful pregnancy and progressively immobilizes the mother. Radiographic studies detect drastic loss of bone mass, elevated rates of turnover in the bone, and oedema in the affected portion. Weakness of the bone can lead to fractures during delivery and other complications for the mother. Then, within weeks of labour, symptoms and radiological findings resolve. Aetiology is currently unknown, although neural, vascular, haematological, endocrine, nutrient-deficiency, and other etiologies have been proposed. Several treatments have also been explored, including simple bed rest, steroids, bisphosphonates, calcitonin, induced termination of pregnancy, and surgical intervention. The orthopedist plays an essential role in monitoring the condition (and potential complications) as well as ensuring satisfactory outcomes for both the mother and newborn. Copyright © 2012 Elsevier Ltd. All rights reserved.

  17. Delivering golden rice to developing countries.

    PubMed

    Mayer, Jorge E

    2007-01-01

    Micronutrient deficiencies create a vicious circle of malnutrition, poverty, and economic dependency that we must strive to break. Golden Rice offers a sustainable solution to reduce the prevalence of vitamin A deficiency-related diseases and mortality, a problem that affects the health of millions of children in all developing countries. The technology is based on the reconstitution of the carotenoid biosynthetic pathway by addition of 2 transgenes. The outcome of this high-tech approach will be provided to end users as nutrient-dense rice varieties that are agronomically identical to their own, locally adapted varieties. This intervention has the potential to reach remote rural populations without access to fortification and supplementation programs. As part of our delivery strategy, we are partnering with government and nongovernment, national and international agricultural institutions to navigate through cumbersome and expensive regulatory regimes that affect the release of genetically modified crops, and to create local demand for the biofortified rice varieties.

  18. Plants adapted to nutrient limitation allocate less biomass into stems in an arid-hot grassland.

    PubMed

    Yan, Bangguo; Ji, Zhonghua; Fan, Bo; Wang, Xuemei; He, Guangxiong; Shi, Liangtao; Liu, Gangcai

    2016-09-01

    Biomass allocation can exert a great influence on plant resource acquisition and nutrient use. However, the role of biomass allocation strategies in shaping plant community composition under nutrient limitations remains poorly addressed. We hypothesized that species-specific allocation strategies can affect plant adaptation to nutrient limitations, resulting in species turnover and changes in community-level biomass allocations across nutrient gradients. In this study, we measured species abundance and the concentrations of nitrogen and phosphorus in leaves and soil nutrients in an arid-hot grassland. We quantified species-specific allocation parameters for stems vs leaves based on allometric scaling relationships. Species-specific stem vs leaf allocation parameters were weighted with species abundances to calculate the community-weighted means driven by species turnover. We found that the community-weighted means of biomass allocation parameters were significantly related to the soil nutrient gradient as well as to leaf stoichiometry, indicating that species-specific allocation strategies can affect plant adaptation to nutrient limitations in the studied grassland. Species that allocate less to stems than leaves tend to dominate nutrient-limited environments. The results support the hypothesis that species-specific allocations affect plant adaptation to nutrient limitations. The allocation trade-off between stems and leaves has the potential to greatly affect plant distribution across nutrient gradients. © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.

  19. Integrated plant nutrient system - with special emphasis on mineral nutriton and biofertilizers for Black pepper and cardamom - A review.

    PubMed

    K P, Sangeeth; R, Suseela Bhai

    2016-05-01

    Integrated Plant Nutrition System (IPNS) as a concept and farm management strategy embraces and transcends from single season crop fertilization efforts to planning and management of plant nutrients in crop rotations and farming systems on a long-term basis for enhanced productivity, profitability and sustainability. It is estimated that about two-thirds of the required increase in crop production in developing countries will have to come from yield increases from lands already under cultivation. IPNS enhances soil productivity through a balanced use of soil nutrients, chemical fertilizers, combined with organic sources of plant nutrients, including bio-inoculants and nutrient transfer through agro-forestry systems and has adaptation to farming systems in both irrigated and rainfed agriculture. Horticultural crops, mainly plantation crops, management practices include application of fertilizers and pesticides which become inevitable due to the depletion of soil organic matter and incidence of pests and diseases. The extensive use of chemical fertilizers in these crops deteriorated soil health that in turn affected the productivity. To revitalize soil health and to enhance productivity, it is inexorable to enrich the soil using microorganisms. The lacunae observed here is the lack of exploitation of indigenous microbes having the potential to fix atmospheric nitrogen (N) and to solubilize Phosphorus (P) and Potassium (K). The concept of biofertilizer application appears to be technically simple and financially feasible, but the task of developing biofertilizers with efficient strains in appropriate combinations in a consortia mode is not easier. More than developing consortia, a suitable delivery system to discharge the microbial inoculants warranted much effort. This review focuses on the integrated plant nutrition system incorporating biofertilizer with special emphasis on developing and formulating biofertilizer consortium.

  20. Important drug-nutrient interactions in the elderly.

    PubMed

    Thomas, J A; Burns, R A

    1998-09-01

    Several drug-nutrient interactions can occur, but their prevalence may be accentuated in the elderly. Geriatric patients may experience age-related changes in the pharmacokinetics of a drug-absorption, distribution, metabolism and excretion. When drug-nutrient interactions occur, they usually affect absorptive processes more frequently. Specific transporter systems facilitate the absorption of many drugs. Little is known about how these transporter systems are affected by aging. Co-existing disease states in the elderly may exaggerate the action of a drug and represent a confounding factor in drug-nutrient interactions. While several different drug-nutrient interactions are important in the elderly, those affecting the cardiovascular system warrant special attention.

  1. Examining Dehydration and Hypoxic Stress in Wheat Plants Using a Porous Tube Plant Nutrient Delivery System Developed for Microgravity

    NASA Technical Reports Server (NTRS)

    Dreschel, T. W.; Hall, C. R.; Foster, T. E.; Salganic, M.; Warren, L.; Corbett, M.

    2005-01-01

    The Porous Tube Plant Nutrient Delivery System (PTPNDS) was designed for NASA to grow plants in microgravity of space. The system utilizes a controlled fluid loop to supply nutrients and water to plant roots growing on a ceramic surface moistened by capiflary action. A PTPNDS test bed was developed and utilizing remote sensing systems, spectral analyses procedures, gas-exchange, and fluorescence measurements, we examined differences in plant water status for wheat plants (Triticum aestivum, cv. Perigee) grown in a modified growth chamber during the summers of 2003 and 2004. Some differences in plant performance were detectable in the gas-exchange and fluorescence measurements. For instance, in both years the plants grown with the most available water had the lowest rates of photosynthesis and exhibited higher proportions of non-photochemical quenching particularly under low light levels. In addition, small differences in mean leaf water content between treatments were detected using spectral reflectance analyses.

  2. Postpyloric regulation of gastric emptying in rhesus monkeys.

    PubMed

    McHugh, P R; Moran, T H; Wirth, J B

    1982-09-01

    Saline (0.9% NaCl) empties rapidly and exponentially from the stomach of the rhesus monkey, but glucose solutions empty at a calorie-constant rate of 0.4 kcal/min. By means of indwelling intragastric and intraduodenal cannulae we can demonstrate an inhibition on the delivery of saline from the stomach provoked by glucose placed beyond the pylorus. The inhibition varies directly with the glucose calories in the intestine and averages 2.5 min/kcal. That these two results (0.4 kcal/min and 2.5 min/kcal) are reciprocals suggests a feedback inhibition on the gastric emptying of nutrients arising from beyond the pylorus and adequate to explain the rate of glucose delivery to the intestine. A control theory description of gastric emptying that includes such feedback regulation can be derived from these data to explain the different gastric emptying patterns of nutrients and nonnutrient solutions. These patterns give this visceral system a precision in its management of nutrients that can provide information crucial to preabsorptive satiety.

  3. Nutrient budgets in the subtropical ocean gyres dominated by lateral transport

    NASA Astrophysics Data System (ADS)

    Letscher, Robert T.; Primeau, François; Moore, J. Keith

    2016-11-01

    Ocean circulation replenishes surface nutrients depleted by biological production and export. Vertical processes are thought to dominate, but estimated vertical nutrient fluxes are insufficient to explain observed net productivity in the subtropical ocean gyres. Lateral inputs help balance the North Atlantic nutrient budget, but their importance for other gyres has not been demonstrated. Here we use an ocean model that couples circulation and ecosystem dynamics to show that lateral transport and biological uptake of inorganic and organic forms of nitrogen and phosphorus from the gyre margins exceeds the vertical delivery of nutrients, supplying 24-36% of the nitrogen and 44-67% of the phosphorus required to close gyre nutrient budgets. At the Bermuda and Hawaii time-series sites, nearly half of the annual lateral supply by lateral transport occurs during the summer-to-fall stratified period, helping explain seasonal patterns of inorganic carbon drawdown and nitrogen fixation. Our study confirms the importance of upper-ocean lateral nutrient transport for understanding the biological cycles of carbon and nutrients in the ocean's largest biome.

  4. Forecasting future phosphorus export to the Laurentian Great Lakes from land-derived nutrient inputs

    NASA Astrophysics Data System (ADS)

    LaBeau, M. B.; Robertson, D. M.; Mayer, A. S.; Pijanowski, B. C.

    2011-12-01

    Anthropogenic use of the land through agricultural and urban activities has significantly increased phosphorus loading to rivers that flow to the Great Lakes. Phosphorus (P) is a critical element in the eutrophication of the freshwater ecosystems, most notably the Great Lakes. To better understand factors influencing phosphorus delivery to aquatic systems and thus their potential harmful effects to lake ecosystems, models that predict P export should incorporate account for changing changes in anthropogenic activities. Land-derived P from high yielding sources, such as agriculture and urban areas, affect eutrophication at various scales (e.g. specific bays to all of Lake Erie). SPARROW (SPAtially Referenced Regression On Watershed attributes) is a spatially explicit watershed model that has been used to understand linkages between land-derived sources and nutrient transport to the Great Lakes. The Great Lakes region is expected to experience a doubling of urbanized areas along with a ten percent increase in agricultural use over the next 40 years, which is likely to increase P loading. To determine how these changes will impact P loading, SPARROW have been developed that relate changes in land use to changes in nutrient sources, including relationships between row crop acreage and fertilizer intensity and urban land use and point source intensity. We used land use projections from the Land Transformation Model, a, spatially explicit, neural-net based land change model. Land use patterns from current to 2040 were used as input into HydroSPARROW, a forecasting tool that enables SPARROW to simulate the effects of various land-use and climate scenarios. Consequently, this work is focusing on understanding the effects of how specific agriculture and urbanization activities affect P loading in the watersheds of the Laurentian Great Lakes to potentially find strategies to reduce the extent and severity of future eutrophication.

  5. Short-term effect of nutrient availability and rainfall distribution on biomass production and leaf nutrient content of savanna tree species.

    PubMed

    Barbosa, Eduardo R M; Tomlinson, Kyle W; Carvalheiro, Luísa G; Kirkman, Kevin; de Bie, Steven; Prins, Herbert H T; van Langevelde, Frank

    2014-01-01

    Changes in land use may lead to increased soil nutrient levels in many ecosystems (e.g. due to intensification of agricultural fertilizer use). Plant species differ widely in their response to differences in soil nutrients, and for savannas it is uncertain how this nutrient enrichment will affect plant community dynamics. We set up a large controlled short-term experiment in a semi-arid savanna to test how water supply (even water supply vs. natural rainfall) and nutrient availability (no fertilisation vs. fertilisation) affects seedlings' above-ground biomass production and leaf-nutrient concentrations (N, P and K) of broad-leafed and fine-leafed tree species. Contrary to expectations, neither changes in water supply nor changes in soil nutrient level affected biomass production of the studied species. By contrast, leaf-nutrient concentration did change significantly. Under regular water supply, soil nutrient addition increased the leaf phosphorus concentration of both fine-leafed and broad-leafed species. However, under uneven water supply, leaf nitrogen and phosphorus concentration declined with soil nutrient supply, this effect being more accentuated in broad-leafed species. Leaf potassium concentration of broad-leafed species was lower when growing under constant water supply, especially when no NPK fertilizer was applied. We found that changes in environmental factors can affect leaf quality, indicating a potential interactive effect between land-use changes and environmental changes on savanna vegetation: under more uneven rainfall patterns within the growing season, leaf quality of tree seedlings for a number of species can change as a response to changes in nutrient levels, even if overall plant biomass does not change. Such changes might affect herbivore pressure on trees and thus savanna plant community dynamics. Although longer term experiments would be essential to test such potential effects of eutrophication via changes in leaf nutrient concentration, our findings provide important insights that can help guide management plans that aim to preserve savanna biodiversity.

  6. From Biological to Program Efficacy: Promoting Dialogue among the Research, Policy, and Program Communities12

    PubMed Central

    Habicht, Jean-Pierre; Pelto, Gretel H.

    2014-01-01

    The biological efficacy of nutritional supplements to complement usual diets in poor populations is well established. This knowledge rests on decades of methodologic research development and, more recently, on codification of methods to compile and interpret results across studies. The challenge now is to develop implementation (delivery) science knowledge and achieve a similar consensus on efficacy criteria for the delivery of these nutrients by public health and other organizations. This requires analysis of the major policy instruments for delivery and well-designed program delivery studies that examine the flow of a nutrient through a program impact pathway. This article discusses the differences between biological and program efficacy, and why elucidating the fidelity of delivery along the program impact pathways is essential for implementing a program efficacy trial and for assessing its internal and external validity. Research on program efficacy is expanding, but there is a lack of adequate frameworks to facilitate the process of harmonizing concepts and vocabulary, which is essential for communication among scientists, policy planners, and program implementers. There is an urgent need to elaborate these frameworks at national and program levels not only for program efficacy studies but also for the broader research agenda to support and improve the science of delivering adequate nutrition to those who need it most. PMID:24425719

  7. Veggie Passive Orbital Nutrient Delivery System (PONDS)

    NASA Image and Video Library

    2018-02-27

    Project scientists, place seeds in Veggie Passive Orbital Nutrient Delivery System (PONDS) units inside a laboratory at the Space Station Processing Facility at NASA’s Kennedy Space Center in Florida. Veggie PONDS is a direct follow-on to the Veg-01 and Veg-03 hardware and plant growth validation tests. The primary goal of this newly developed plant growing system, Veggie PONDS, is to demonstrate uniform plant growth. PONDS units have features that are designed to mitigate microgravity effects on water distribution, increase oxygen exchange and provide sufficient room for root zone growth. PONDS is planned for use during Veg-04 and Veg-05 on the International Space Station after the Veggie PONDS Validation flights on SpaceX-14 and OA-9.

  8. Technology for subsystems of space-based plant growth facilities

    NASA Technical Reports Server (NTRS)

    Bula, R. J.; Morrow, R. C.; Tibbitts, T. W.; Corey, R. B.

    1990-01-01

    Technologies for different subsystems of space-based plant growth facilities are being developed at the Wisconsin Center for Space Automation and Robotics, a NASA Center for the Commercial Development of Space. The technologies include concepts for water and nutrient delivery, for nutrient composition control, and for irradiation. Effort is being concentrated on these subsystems because available technologies cannot be effectively utilized for space applications.

  9. Application of a calibrated/validated Agricultural Policy/Environmental eXtender model to assess sediment and nutrient delivery from the Wildcat Creek Mississippi River Basin Initiative – Cooperative Conservation Partnership

    USDA-ARS?s Scientific Manuscript database

    The Wildcat Creek, a tributary to the Wabash River was identified by the USDA Natural Resources Conservation Service (NRCS) as a priority watershed for its high sediment and nutrient loading contributions to the Mississippi River. As part of the Mississippi River Basin Initiative (MRBI), the incorpo...

  10. Predicting nitrogen loading with land-cover composition: how can watershed size affect model performance?

    PubMed

    Zhang, Tao; Yang, Xiaojun

    2013-01-01

    Watershed-wide land-cover proportions can be used to predict the in-stream non-point source pollutant loadings through regression modeling. However, the model performance can vary greatly across different study sites and among various watersheds. Existing literature has shown that this type of regression modeling tends to perform better for large watersheds than for small ones, and that such a performance variation has been largely linked with different interwatershed landscape heterogeneity levels. The purpose of this study is to further examine the previously mentioned empirical observation based on a set of watersheds in the northern part of Georgia (USA) to explore the underlying causes of the variation in model performance. Through the combined use of the neutral landscape modeling approach and a spatially explicit nutrient loading model, we tested whether the regression model performance variation over the watershed groups ranging in size is due to the different watershed landscape heterogeneity levels. We adopted three neutral landscape modeling criteria that were tied with different similarity levels in watershed landscape properties and used the nutrient loading model to estimate the nitrogen loads for these neutral watersheds. Then we compared the regression model performance for the real and neutral landscape scenarios, respectively. We found that watershed size can affect the regression model performance both directly and indirectly. Along with the indirect effect through interwatershed heterogeneity, watershed size can directly affect the model performance over the watersheds varying in size. We also found that the regression model performance can be more significantly affected by other physiographic properties shaping nitrogen delivery effectiveness than the watershed land-cover heterogeneity. This study contrasts with many existing studies because it goes beyond hypothesis formulation based on empirical observations and into hypothesis testing to explore the fundamental mechanism.

  11. Novel starch based nano scale enteric coatings from soybean meal for colon-specific delivery.

    PubMed

    Sivapragasam, Nilushni; Thavarajah, Pushparajah; Ohm, Jae-Bom; Ohm, Jae-Bom; Margaret, Khaitsa; Thavarajah, Dil

    2014-10-13

    Soybean meal was used to isolate resistant starch and produce nanoparticles, which could be potential coating materials for colonic nutrient and drug deliveries. The nanoparticles were in 40 ± 33.2 nm ranges. These nanoparticles were stable under simulated human physiological conditions. The degrees of dissolution in both stomach and intestinal conditions were less than 30%. Furthermore, the nanoparticles were less susceptible to pancreatic enzymatic digestion (20%), which was also evidenced by the co-existence of B-type crystalline pattern. In addition to the dissolution and digestion studies in the upper gastrointestinal tract, the nanoparticles were subjected to in vitro fermentation by Bifidobacterium brevis and Lactobacillus casei. Both species showed an increase in growth and activity, while producing short chain fatty acids: acetate, propionate, and butyrates in varying amounts. Overall this study clearly demonstrated a novel method that can be used for colon-specific delivery of bioactive compounds such as drugs and nutrients. Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. A Holocene record of climate-driven shifts in coastal carbon sequestration

    USGS Publications Warehouse

    Mitra, Siddhartha; Zimmerman, A.R.; Hunsinger, G.B.; Willard, D.; Dunn, J.C.

    2009-01-01

    A sediment core collected in the mesohaline portion of Chesapeake Bay was found to contain periods of increased delivery of refractory black carbon (BC) and polycyclic aromatic hydrocarbons (PAHs). The BC was most likely produced by biomass combustion during four centennialscale dry periods as indicated by the Palmer Drought Severity Index (PDSI), beginning in the late Medieval Warm Period of 1100 CE. In contrast, wetter periods were associated with increased non-BC organic matter influx into the bay, likely due to greater runoff and associated nutrient delivery. In addition, an overall increase in both BC and non-BC organic matter deposition during the past millennium may reflect a shift in climate regime. The finding that carbon sequestration in the coastal zone responds to climate fluctuations at both centennial and millennial scales through fire occurrence and nutrient delivery has implications for past and future climate predictions. Drought-induced fires may lead, on longer timescales, to greater carbon sequestration and, therefore, represent a negative climate feedback. Copyright 2009 by the American Geophysical Union.

  13. Drug-nutrient interactions.

    PubMed

    Trovato, A; Nuhlicek, D N; Midtling, J E

    1991-11-01

    Drug-nutrient interactions are a commonly overlooked aspect of the prescribing practices of physicians. As more pharmaceutical agents become available, attention should be focused on interactions of drugs with foods and nutrients. Although drug-nutrient interactions are not as common as drug-drug interactions, they can have an impact on therapeutic outcome. Drugs can affect nutritional status by altering nutrient absorption, metabolism, utilization or excretion. Food, beverages and mineral or vitamin supplements can affect the absorption and effectiveness of drugs. Knowledge of drug-nutrient interactions can help reduce the incidence of these effects. Physicians should question patients about their dietary habits so that patients can be informed about possible interactions between a prescribed drug and foods and nutrients.

  14. Short-term effects of a simulated acid rain upon the growth and nutrient relations of Pinus strobus, L.

    Treesearch

    Tim Wood; F. H. Bormann

    1976-01-01

    Acidified precipitation may affect the productivity of forests by altering the availability of plant nutrients of by affecting the ability of trees to absorb and assimilate those nutrients. In this study, the short-term effects of simulated acid rain (pH range 5.6 - 2.3) upon the growth and nutrient relations of Eastern White Pine seedlings (Pinus strobus...

  15. Apparatus and method for phosphate-accelerated bioremediation

    DOEpatents

    Looney, Brian B.; Pfiffner, Susan M.; Phelps, Tommy J.; Lombard, Kenneth H.; Hazen, Terry C.; Borthen, James W.

    1998-01-01

    An apparatus and method for supplying a vapor-phase nutrient to contaminated soil for in situ bioremediation. The apparatus includes a housing adapted for containing a quantity of the liquid nutrient, a conduit in communication with the interior of the housing, means for causing a gas to flow through the conduit, and means for contacting the gas with the liquid so that a portion thereof evaporates and mixes with the gas. The mixture of gas and nutrient vapor is delivered to the contaminated site via a system of injection and extraction wells configured to the site and provides for the use of a passive delivery system. The mixture has a partial pressure of vaporized nutrient that is no greater than the vapor pressure of the liquid. If desired, the nutrient and/or the gas may be heated to increase the vapor pressure and the nutrient concentration of the mixture. Preferably, the nutrient is a volatile, substantially nontoxic and nonflammable organic phosphate that is a liquid at environmental temperatures, such as triethyl phosphate or tributyl phosphate.

  16. Development of a Self-calibrating Dissolved Oxygen Microsensor Array for the Monitoring and Control of Plant Growth in a Space Environment

    NASA Technical Reports Server (NTRS)

    Kim, Chang-Soo; Brown, Christopher S.; Nagle, H. Troy

    2004-01-01

    Plant experiments in space will require active nutrient delivery concepts in which water and nutrients are replenished on a continuous basis for long-term growth. The goal of this study is to develop a novel microsensor array to provide information on the dissolved oxygen environment in the plant root zone for the optimum control of plant cultivation systems in the space environment. Control of water and oxygen is limited by the current state-of-the-art in sensor technology. Two capabilities of the new microsensor array were tested. First, a novel in situ self-diagnosis/self-calibration capability for the microsensor was explored by dynamically controlling the oxygen microenvironment in close proximity to an amperometric dissolved oxygen microsensors. A pair of integrated electrochemical actuator electrodes provided the microenvironments based on water electrolysis. Miniaturized thin film dissolved oxygen microsensors on a flexible polyimide (Kapton(Registered Trademark)? substrate were fabricated and their performances were tested. Secondly, measurements of dissolved oxygen in two representative plant growth systems were made, which had not been performed previously due to lack of proper sensing technology. The responses of the oxygen microsensor array on a flexible polymer substrate properly reflected the oxygen contents on the surface of a porous tube nutrient delivery system and within a particulate substrate system. Additionally, we demonstrated the feasibility of using a 4-point thin film microprobe for water contents measurements for both plant growth systems. mechanical flexibility, and self-diagnosis. The proposed technology is anticipated to provide a reliable sensor feedback plant growth nutrient delivery systems in both terrestrial environment and the microgravity environment during long term space missions. The unique features of the sensor include small size and volume, multiple-point sensing,

  17. Controlling Salmonella infection in weanling pigs through water delivery of direct-fed microbials or organic acids: Part II. Effects on intestinal histology and active nutrient transport

    USDA-ARS?s Scientific Manuscript database

    The objective of this study was to evaluate the effects of water-delivered direct-fed microbials (DFM) or organic acids on intestinal morphology and active nutrient absorption in weanling pigs following deliberate Salmonella infection. Pigs (n = 88) were weaned at 19 ± 2 d of age and assigned to one...

  18. Concept for Sustained Plant Production on ISS Using VEGGIE Capillary Mat Rooting System

    NASA Technical Reports Server (NTRS)

    Stutte, Gary W.; Newsham, Gerard; Morrow, Robert M.; Wheeler, Raymond M.

    2011-01-01

    Plant growth in microgravity presents unique challenges associated with maintaining appropriate conditions for seed germination, seedling establishment, maturation and harvest. They include maintaining appropriate soil moisture content, nutrient balance, atmospheric mixing and containment. Sustained production imposes additional challenges of harvesting, replanting, and safety. The VEGGIE is a deployable (collapsible) plant growth chamber developed as part of a NASA SBIR Phase II by Orbitec, Madison, WI. The intent of VEGGIE is to provide a low-resource system to produce fresh vegetables for the crew on long duration missions. The VEGGIE uses and LED array for lighting, an expandable bellows for containment, and a capillary matting system for nutrient and water delivery. The project evaluated a number of approaches to achieve sustained production, and repeated plantings, using the capillary rooting system. A number of different root media, seed containment, and nutrient delivery systems were evaluated and effects on seed germination and growth were evaluated. A number of issues limiting sustained production, such as accumulation of nutrients, uniform water, elevated vapor pressure deficit, and media containment were identified. A concept using pre-planted rooting packs shown to effectively address a number of those issues and is a promising approach for future development as a planting system for microgravity conditions.

  19. Exploring spatiotemporal changes of the Yangtze River (Changjiang) nitrogen and phosphorus sources, retention and export to the East China Sea and Yellow Sea.

    PubMed

    Liu, Xiaochen; Beusen, Arthur H W; Van Beek, Ludovicus P H; Mogollón, José M; Ran, Xiangbin; Bouwman, Alexander F

    2018-06-04

    Nitrogen (N) and phosphorus (P) flows from land to sea in the Yangtze River basin were simulated for the period 1900-2010, by combining models for hydrology, nutrient input to surface water, and an in-stream retention. This study reveals that the basin-wide nutrient budget, delivery to surface water, and in-stream retention increased during this period. Since 2004, the Three Gorges Reservoir has contributed 5% and 7% of N and P basin-wide retention, respectively. With the dramatic rise in nutrient delivery, even this additional retention was insufficient to prevent an increase of riverine export from 337 Gg N yr -1 and 58 Gg P yr -1 (N:P molar ratio = 13) to 5896 Gg N yr -1 and 381 Gg P yr -1 (N:P molar ratio = 35) to the East China Sea and Yellow Sea (ECSYS). The midstream and upstream subbasins dominate the N and P exports to the ECSYS, respectively, due to various human activities along the river. Our spatially explicit nutrient source allocation can aid in the strategic targeting of nutrient reduction policies. We posit that these should focus on improving the agricultural fertilizer and manure use efficiency in the upstream and midstream and better urban wastewater management in the downstream subbasin. Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.

  20. Nutrient losses from fall- and winter-applied manure: effects of timing and soil temperature

    USDA-ARS?s Scientific Manuscript database

    Soil temperature is a major environmental factor that affects meltwater and precipitation infiltration and nutrient cycling. The objective of this study was to determine nutrient losses in runoff and leachate from fall- and winter-applied dairy manure as affected by soil temperature at the time of a...

  1. Impact of direct drug delivery via gastric access devices.

    PubMed

    Kurien, Matthew; Penny, Hugo; Sanders, David S

    2015-03-01

    Gastric access devices such as nasogastric tubes and gastrostomy tubes are increasingly being used in clinical practice to provide both short- and long-term nutrition support therapy. Increasingly these devices are being utilized to help deliver oral medications, where swallowing is impaired. This concomitant administration of medications and enteral formulas could derive potential benefits in regard to time and cost; however, uncertainty exists regarding potential drug and nutrient interactions and the influence this may have on both safety and efficacy. This article provides an overview of the differing gastric access devices used in clinical practice and evaluates the evidence base for using oral medications via these routes. Alternative methods of drug administration are discussed, alongside common drug nutrient interactions and potential complications. Delivering medications via gastric access devices can be performed safely; however, careful consideration needs to be made regarding tube and patient influences, alongside drug-nutrient interactions. Improving practice in this area in the future necessitates enhancement of an evidence base to substantiate the safety of drug delivery via gastric access devices and improvement in education among healthcare professionals about the potential problems.

  2. Maternal predictors of intrauterine growth restriction.

    PubMed

    Cetin, Irene; Mandò, Chiara; Calabrese, Stefania

    2013-05-01

    Intrauterine growth restriction (IUGR) occurs when fetal growth rate falls below the genetic potential and affects a significant number of pregnancies, but still no therapy has been developed for this pregnancy disease. This article reviews the most recent findings concerning maternal characteristics and behaviours predisposing to IUGR as well as maternal early markers of the disease. A comprehensive understanding of factors associated with IUGR will help in providing important tools for preventing and understanding adverse outcomes. Maternal nutritional status, diet and exposure to environmental factors are increasingly acknowledged as potential factors affecting fetal growth both by altering nutrient availability to the fetus and by modulating placental gene expression, thus modifying placental function. Assessing nutritional and environmental factors associated with IUGR, and the molecular mechanisms by which they may have a role in the disease onset, is necessary to provide comprehensive and common guidelines for maternal care and recommended behaviours. Moreover, maternal genetic predispositions and early serum markers may allow a better and more specific monitoring of high risk pregnancies, optimizing the timing of delivery.

  3. Peer-facilitated cognitive dissonance versus healthy weight eating disorders prevention: A randomized comparison.

    PubMed

    Becker, Carolyn Black; Wilson, Chantale; Williams, Allison; Kelly, Mackenzie; McDaniel, Leda; Elmquist, Joanna

    2010-09-01

    Research supports the efficacy of both cognitive dissonance (CD) and healthy weight (HW) eating disorders prevention, and indicates that CD can be delivered by peer-facilitators, which facilitates dissemination. This study investigated if peer-facilitators can deliver HW when it is modified for their use and extended follow-up of peer-facilitated CD as compared to previous trials. Based on pilot data, we modified HW (MHW) to facilitate peer delivery, elaborate benefits of the healthy-ideal, and place greater emphasis on consuming nutrient dense foods. Female sorority members (N=106) were randomized to either two 2-h sessions of CD or MHW. Participants completed assessment pre- and post-intervention, and at 8-week, 8-month, and 14-month follow-up. Consistent with hypotheses, CD decreased negative affect, thin-ideal internalization, and bulimic pathology to a greater degree post-intervention. Both CD and MHW reduced negative affect, internalization, body dissatisfaction, dietary restraint, and bulimic pathology at 14 months. Copyright © 2010 Elsevier Ltd. All rights reserved.

  4. Spatial and Temporal Patterns of Chlorophyll Concentration in the Southern California Bight

    NASA Astrophysics Data System (ADS)

    Nezlin, Nikolay P.; McLaughlin, Karen; Booth, J. Ashley T.; Cash, Curtis L.; Diehl, Dario W.; Davis, Kristen A.; Feit, Adriano; Goericke, Ralf; Gully, Joseph R.; Howard, Meredith D. A.; Johnson, Scott; Latker, Ami; Mengel, Michael J.; Robertson, George L.; Steele, Alex; Terriquez, Laura; Washburn, Libe; Weisberg, Stephen B.

    2018-01-01

    Distinguishing between local, anthropogenic nutrient inputs and large-scale climatic forcing as drivers of coastal phytoplankton biomass is critical to developing effective nutrient management strategies. Here we assess the relative importance of these two drivers by comparing trends in chlorophyll-a between shallow coastal (0.1-16.5 km) and deep offshore (17-700 km) areas, hypothesizing that coastal regions influenced by anthropogenic nutrient inputs may have different spatial and temporal patterns in chlorophyll-a concentration from offshore regions where coastal inputs are less influential. Quarterly conductivity-temperature-depth (CTD) fluorescence measurements collected from three southern California continental shelf regions since 1998 were compared to chlorophyll-a data from the more offshore California Cooperative Fisheries Investigations (CalCOFI) program. The trends in the coastal zone were similar to those offshore, with a gradual increase of chlorophyll-a biomass and shallowing of its maximum layer since the beginning of observations, followed by chlorophyll-a declining and deepening from 2010 to present. An exception was the northern coastal part of SCB, where chlorophyll-a continued increasing after 2010. The long-term increase in chlorophyll-a prior to 2010 was correlated with increased nitrate concentrations in deep waters, while the recent decline was associated with deepening of the upper mixed layer, both linked to the low-frequency climatic cycles of the Pacific Decadal Oscillation and North Pacific Gyre Oscillation. These large-scale factors affecting the physical structure of the water column may also influence the delivery of nutrients from deep ocean outfalls to the euphotic zone, making it difficult to distinguish the effects of anthropogenic inputs on chlorophyll along the coast.

  5. Prenatal physical activity and diet composition affect the expression of nutrient transporters and mTOR signaling molecules in the human placenta.

    PubMed

    Brett, K E; Ferraro, Z M; Holcik, M; Adamo, K B

    2015-02-01

    Adequate nutrient delivery to the fetus is essential for optimal growth. Differences in prenatal physical activity level and diet quality influence maternal energy balance and these factors may alter placental nutrient transport. We investigated the associations between meeting physical activity guidelines and the quality of maternal diet on the expression of genes involved in fatty acid, amino acid and glucose transport, and mammalian target of rapamycin (mTOR) and insulin signaling in the placenta from 16 term pregnancies. Physical activity was directly measured with accelerometry, diet composition was assessed with 24 h dietary recalls, and gene expression was measured with custom polymerase chain reaction (PCR) arrays. Women who met physical activity guidelines had lower gene expression of fatty acid transport protein 4 (FATP4), insulin-like growth factor 1 (IGF1), and the beta non-catalytic subunit of AMP-activated protein kinase (AMPK), and a higher expression of SNAT2. There was a strong positive correlation observed between total sugar intake and glucose transporter 1 (GLUT1) (r = 0.897, p = 0.000, n = 12), and inverse correlations between total sugar and mTOR and IGF1 expression. Percentage of total calories from protein was inversely related to insulin-like growth factor 1 receptor (IGF1R) (r = -0.605, p = 0.028, n = 13). Variations in maternal physical activity and diet composition altered the expression of genes involved in fatty acid, amino acid and glucose transport and mTOR signaling. Future research on placental nutrient transport should include direct measures of maternal PA and dietary habits to help eliminate confounding factors. Copyright © 2014 Elsevier Ltd. All rights reserved.

  6. Hypothalamic nutrient sensing activates a forebrain-hindbrain neuronal circuit to regulate glucose production in vivo.

    PubMed

    Lam, Carol K L; Chari, Madhu; Rutter, Guy A; Lam, Tony K T

    2011-01-01

    Hypothalamic nutrient sensing regulates glucose production, but the neuronal circuits involved remain largely unknown. Recent studies underscore the importance of N-methyl-d-aspartate (NMDA) receptors in the dorsal vagal complex in glucose regulation. These studies raise the possibility that hypothalamic nutrient sensing activates a forebrain-hindbrain NMDA-dependent circuit to regulate glucose production. We implanted bilateral catheters targeting the mediobasal hypothalamus (MBH) (forebrain) and dorsal vagal complex (DVC) (hindbrain) and performed intravenous catheterizations to the same rat for infusion and sampling purposes. This model enabled concurrent selective activation of MBH nutrient sensing by either MBH delivery of lactate or an adenovirus expressing the dominant negative form of AMPK (Ad-DN AMPK α2 [D¹⁵⁷A]) and inhibition of DVC NMDA receptors by either DVC delivery of NMDA receptor blocker MK-801 or an adenovirus expressing the shRNA of NR1 subunit of NMDA receptors (Ad-shRNA NR1). Tracer-dilution methodology and the pancreatic euglycemic clamp technique were performed to assess changes in glucose kinetics in the same conscious, unrestrained rat in vivo. MBH lactate or Ad-DN AMPK with DVC saline increased glucose infusion required to maintain euglycemia due to an inhibition of glucose production during the clamps. However, DVC MK-801 negated the ability of MBH lactate or Ad-DN AMPK to increase glucose infusion or lower glucose production. Molecular knockdown of DVC NR1 of NMDA receptor via Ad-shRNA NR1 injection also negated MBH Ad-DN AMPK to lower glucose production. Molecular and pharmacological inhibition of DVC NMDA receptors negated hypothalamic nutrient sensing mechanisms activated by lactate metabolism or AMPK inhibition to lower glucose production. Thus, DVC NMDA receptor is required for hypothalamic nutrient sensing to lower glucose production and that hypothalamic nutrient sensing activates a forebrain-hindbrain circuit to lower glucose production.

  7. Porous membrane utilization in plant nutrient delivery

    NASA Technical Reports Server (NTRS)

    Dreschel, T. W.; Hinkle, C. R.; Prince, R. P.; Knott, W. M., III

    1987-01-01

    A spacecraft hydroponic plant growth unit of tubular configuration, employing a microporous membrane as a capilary interface between plant roots and a nutrient solution, is presented. All three of the experimental trials undertaken successfully grew wheat from seed to harvest. Attention is given to the mass/seed, number of seeds/head, ratio of seed dry mass to total plant dry mass, production of tillers, and mass of seed/plant. Dry matter production is found to be reduced with increasing suction pressure; this is true for both average seed and average total dry matter/plant. This may be due to a reduction in water and nutrient availability through the microporous membrane.

  8. Apparatus and method for phosphate-accelerated bioremediation

    DOEpatents

    Looney, B.B.; Pfiffner, S.M.; Phelps, T.J.; Lombard, K.H.; Hazen, T.C.; Borthen, J.W.

    1998-05-19

    An apparatus and method are provided for supplying a vapor-phase nutrient to contaminated soil for in situ bioremediation. The apparatus includes a housing adapted for containing a quantity of the liquid nutrient, a conduit in communication with the interior of the housing, means for causing a gas to flow through the conduit, and means for contacting the gas with the liquid so that a portion evaporates and mixes with the gas. The mixture of gas and nutrient vapor is delivered to the contaminated site via a system of injection and extraction wells configured to the site and provides for the use of a passive delivery system. The mixture has a partial pressure of vaporized nutrient that is no greater than the vapor pressure of the liquid. If desired, the nutrient and/or the gas may be heated to increase the vapor pressure and the nutrient concentration of the mixture. Preferably, the nutrient is a volatile, substantially nontoxic and nonflammable organic phosphate that is a liquid at environmental temperatures, such as triethyl phosphate or tributyl phosphate. 8 figs.

  9. ASTROCULTURE (TM) root metabolism and cytochemical analysis

    NASA Technical Reports Server (NTRS)

    Porterfield, D. M.; Barta, D. J.; Ming, D. W.; Morrow, R. C.; Musgrave, M. E.

    2000-01-01

    Physiology of the root system is dependent upon oxygen availability and tissue respiration. During hypoxia nutrient and water acquisition may be inhibited, thus affecting the overall biochemical and physiological status of the plant. For the Astroculture (TM) plant growth hardware, the availability of oxygen in the root zone was measured by examining the changes in alcohol dehydrogenase (ADH) activity within the root tissue. ADH activity is a sensitive biochemical indicator of hypoxic conditions in plants and was measured in both spaceflight and control roots. In addition to the biochemical enzyme assays, localization of ADH in the root tissue was examined cytochemically. The results of these analyses showed that ADH activity increased significantly as a result of spaceflight exposure. Enzyme activity increased 248% to 304% in dwarf wheat when compared with the ground controls and Brassica showed increases between 334% and 579% when compared with day zero controls. Cytochemical staining revealed no differences in ADH tissue localization in any of the dwarf wheat treatments. These results show the importance of considering root system oxygenation in designing and building nutrient delivery hardware for spaceflight plant cultivation and confirm previous reports of an ADH response associated with spaceflight exposure.

  10. Bioregenerative life support systems for microgravity

    NASA Technical Reports Server (NTRS)

    Nevill, Gail E., Jr.; Hessel, Michael I., Jr.; Rodriguez, Jose; Morgan, Steve (Editor)

    1993-01-01

    NASA's Controlled Ecological Life Support System (CELSS) project centers on growing plants and recycling wastes in space. The current version of the biomass production chamber (BPC) uses a hydroponic system for nutrient delivery. To optimize plant growth and conserve system resources, the content of the nutrient solution which feeds the plants must be constantly monitored. The macro-nutrients (greater than ten ppm) in the solution include nitrogen, phosphorous, potassium, calcium, magnesium, and sulphur; the micro-nutrients (less than ten ppm) include iron, copper, manganese, zinc, and boron. The goal of this project is to construct a computer-controlled system of ion detectors that will accurately measure the concentrations of several necessary ions in solution. The project focuses on the use of a sensor array to eliminate problems of interference and temperature dependence.

  11. Insight into litter decomposition driven by nutrient demands of symbiosis system through the hypha bridge of arbuscular mycorrhizal fungi.

    PubMed

    Kong, Xiangshi; Jia, Yanyan; Song, Fuqiang; Tian, Kai; Lin, Hong; Bei, Zhanlin; Jia, Xiuqin; Yao, Bei; Guo, Peng; Tian, Xingjun

    2018-02-01

    Arbuscular mycorrhizal fungi (AMF) play an important role in litter decomposition. This study investigated how soil nutrient level affected the process. Results showed that AMF colonization had no significant effect on litter decomposition under normal soil nutrient conditions. However, litter decomposition was accelerated significantly under lower nutrient conditions. Soil microbial biomass in decomposition system was significantly increased. Especially, in moderate lower nutrient treatment (condition of half-normal soil nutrient), litters exhibited the highest decomposition rate, AMF hypha revealed the greatest density, and enzymes (especially nitrate reductase) showed the highest activities as well. Meanwhile, the immobilization of nitrogen (N) in the decomposing litter remarkably decreased. Our results suggested that the roles AMF played in ecosystem were largely affected by soil nutrient levels. At normal soil nutrient level, AMF exhibited limited effects in promoting decomposition. When soil nutrient level decreased, the promoting effect of AMF on litter decomposition began to appear, especially on N mobilization. However, under extremely low nutrient conditions, AMF showed less influence on decomposition and may even compete with decomposer microorganisms for nutrients.

  12. Providing intraosseous anesthesia with minimal invasion.

    PubMed

    Giffin, K M

    1994-08-01

    A new variation of intraosseous anesthesia--crestal anesthesia--that is rapid, site-specific and minimally invasive is presented. The technique uses alveolar crest nutrient canals for anesthetic delivery without penetrating either bone or periodontal ligament.

  13. Inflatable Aeroponic System

    NASA Technical Reports Server (NTRS)

    Pelt, Jennifer Van

    2005-01-01

    Aeroponics Internationals (AI) innovation is a self-contained, self-supporting, flexible low mass aeroponic crop production unit with integral environmental systems for the control and delivery of a nutrient mist to the roots. This FLEX Aeroponic System model was developed for commercialization as a result of the NASA SBIR Phase I contract for the research and development of a low-mass, Inflatable Aeroponic System (IAS) for producing pesticide-free lettuces, grains, peppers, tomatoes and other vegetables. The innovation addresses the needs of water and nutrient delivery systems technologies for food production in space. The inflatable nature of the innovation makes it lightweight, allowing it to be deflated so it takes up less volume during transportation and storage. It improves upon AI's current aeroponic system design that uses more rigid structures and takes advantage of vertical inclines to increase bio-mass production by over 600%.

  14. Using Hyperspectral Aircraft Remote Sensing to Support Ecosystems Services Research in New England Lakes and Ponds

    NASA Astrophysics Data System (ADS)

    Keith, D. J.; Milstead, B.; Walker, H.; Worthy, D.; Szykman, J.; Wusk, M.; Kagey, L.; Howell, C.; Snook, H.; Drueke, C.

    2010-12-01

    Northeastern lakes and ponds provide important ecosystem services to New England residents and visitors. These include the provisioning of abundant, clean water for consumption, agriculture, and industry as well as cultural services (recreation, aesthetics, and wilderness experiences) which enhance local economies and quality of life. Less understood, but equally important, are the roles that these lakes play in protecting all life through supportive services such as nutrient cycling. Nitrogen and phosphorus have a direct impact on the condition of fresh water lakes. Excesses of these nutrients can lead to eutrophication, toxic cyanobacteria blooms, decreased biodiversity, and loss of ecosystem function leading to a reduction in the availability and delivery of ecosystem services. In this study, we examined how variations in lake nutrient concentrations and phytoplankton pigment concentrations correlated with changes in the potential to provide cultural ecosystem services. Using a NASA Cessna 206 aircraft, hyperspectral data were collected during late summer 2009 from 55 lakes in New Hampshire, Massachusetts, Connecticut, and Rhode Island over a 2 day period. From the spectral data, algorithms were created which estimated concentrations of chlorophyll a, phycocyanin, and colored dissolved organic matter. The remotely sensed estimates were supplemented by in situ chlorophyll a, total nitrogen, total phosphorus and lake color data from 43 lakes sampled by field crews from the New England states. The purpose of this research is to understand how variations in lake nutrient concentrations and phytoplankton pigment concentrations correlate with changes in availability of cultural ecosystem services in the surveyed lakes. This dataset will be combined with information from the EPA National Lake Survey (2007), the EPA New England Lakes and Ponds Survey (2008) and the USGS SPARROW model to explore the association between lake condition and the provisioning of ecosystem services on a regional scale. Under the EPA Ecological Services Research Program (ESRP), this information will provide managers and researchers with a better understanding of links between management decisions affecting nutrient fluxes and impacts on selected ecosystem services.

  15. Human Milk Microbial Community Structure Is Relatively Stable and Related to Variations in Macronutrient and Micronutrient Intakes in Healthy Lactating Women

    PubMed Central

    Carrothers, Janae M; Lackey, Kimberly A; Beatty, Nicola F; York, Mara A; Brooker, Sarah L; Settles, Matthew L; McGuire, Mark A

    2017-01-01

    Background: The human milk microbiome has been somewhat characterized, but little is known about changes over time and relations with maternal factors such as nutrient intake. Objective: We sought to characterize the human milk microbiome and described associations with maternal nutrient intake, time postpartum, delivery mode, and body mass index (BMI; in kg/m2). Methods: Milk samples (n = 104) and 24-h diet recalls were collected 9 times from 21 healthy lactating women from day 2 to 6 mo postpartum. Women were classified by BMI as healthy weight (<25) or overweight or obese (≥25). Bacterial taxa were characterized with the use of the high-throughput sequencing of the 16S ribosomal RNA gene. Results: The milk microbiome was relatively constant over time, although there were small changes in some of the lesser-abundant genera. Relative abundances of several taxa were associated with BMI, delivery mode, and infant sex. For instance, overweight and obese mothers produced milk with a higher relative abundance of Granulicatella than did healthy-weight women (1.8% ± 0.6% compared with 0.4% ± 0.2%, respectively; P < 0.05). Relative abundances of several bacterial taxa were also associated with variations in maternal dietary intake. For example, intakes of saturated fatty acids (rs = −0.59; P = 0.005) and monounsaturated fatty acids (rs = −0.46; P = 0.036) were inversely associated with the relative abundance of Corynebacterium; total carbohydrates (rs = −0.54; P = 0.011), disaccharides (rs = −0.47; P = 0.031), and lactose (rs = −0.51; P = 0.018) were negatively associated with Firmicutes; and protein consumption was positively correlated with the relative abundance of Gemella (rs = 0.46; P = 0.037). Conclusions: Factors associated with variations in the human milk microbiome are complex and may include maternal nutrient intake, maternal BMI, delivery mode, and infant sex. Future studies designed to investigate the relation between maternal nutrient intake and the milk microbiome should strive to also evaluate dietary supplement usage and analyze the collected milk for its nutrient content. PMID:28724659

  16. Human Milk Microbial Community Structure Is Relatively Stable and Related to Variations in Macronutrient and Micronutrient Intakes in Healthy Lactating Women.

    PubMed

    Williams, Janet E; Carrothers, Janae M; Lackey, Kimberly A; Beatty, Nicola F; York, Mara A; Brooker, Sarah L; Shafii, Bahman; Price, William J; Settles, Matthew L; McGuire, Mark A; McGuire, Michelle K

    2017-09-01

    Background: The human milk microbiome has been somewhat characterized, but little is known about changes over time and relations with maternal factors such as nutrient intake. Objective: We sought to characterize the human milk microbiome and described associations with maternal nutrient intake, time postpartum, delivery mode, and body mass index (BMI; in kg/m 2 ). Methods: Milk samples ( n = 104) and 24-h diet recalls were collected 9 times from 21 healthy lactating women from day 2 to 6 mo postpartum. Women were classified by BMI as healthy weight (<25) or overweight or obese (≥25). Bacterial taxa were characterized with the use of the high-throughput sequencing of the 16S ribosomal RNA gene. Results: The milk microbiome was relatively constant over time, although there were small changes in some of the lesser-abundant genera. Relative abundances of several taxa were associated with BMI, delivery mode, and infant sex. For instance, overweight and obese mothers produced milk with a higher relative abundance of Granulicatella than did healthy-weight women (1.8% ± 0.6% compared with 0.4% ± 0.2%, respectively; P < 0.05). Relative abundances of several bacterial taxa were also associated with variations in maternal dietary intake. For example, intakes of saturated fatty acids ( r s = -0.59; P = 0.005) and monounsaturated fatty acids ( r s = -0.46; P = 0.036) were inversely associated with the relative abundance of Corynebacterium ; total carbohydrates ( r s = -0.54; P = 0.011), disaccharides ( r s = -0.47; P = 0.031), and lactose ( r s = -0.51; P = 0.018) were negatively associated with Firmicutes; and protein consumption was positively correlated with the relative abundance of Gemella ( r s = 0.46; P = 0.037). Conclusions: Factors associated with variations in the human milk microbiome are complex and may include maternal nutrient intake, maternal BMI, delivery mode, and infant sex. Future studies designed to investigate the relation between maternal nutrient intake and the milk microbiome should strive to also evaluate dietary supplement usage and analyze the collected milk for its nutrient content.

  17. Nutrient concentrations and loads in the northeastern United States - Status and trends, 1975-2003

    USGS Publications Warehouse

    Trench, Elaine C. Todd; Moore, Richard B.; Ahearn, Elizabeth A.; Mullaney, John R.; Hickman, R. Edward; Schwarz, Gregory E.

    2012-01-01

    The U.S. Geological Survey (USGS) National Water-Quality Assessment Program (NAWQA) began regional studies in 2003 to synthesize information on nutrient concentrations, trends, stream loads, and sources. In the northeastern United States, a study area that extends from Maine to central Virginia, nutrient data were evaluated for 130 USGS water-quality monitoring stations. Nutrient data were analyzed for trends in flow-adjusted concentrations, modeled instream (non-flow-adjusted) concentrations, and stream loads for 32 stations with 22 to 29 years of water-quality and daily mean streamflow record during 1975-2003 (termed the long-term period), and for 46 stations during 1993-2003 (termed the recent period), by using a coupled statistical model of streamflow and water quality developed by the USGS. Recent trends in flow-adjusted concentrations of one or more nutrients also were analyzed for 90 stations by using Tobit regression. Annual stream nutrient loads were estimated, and annual nutrient yields were calculated, for 47 stations for the long-term and recent periods, and for 37 additional stations that did not have a complete streamflow and water-quality record for 1993-2003. Nutrient yield information was incorporated for 9 drainage basins evaluated in a national NAWQA study, for a total of 93 stations evaluated for nutrient yields. Long-term downward trends in flow-adjusted concentrations of total nitrogen and total phosphorus (18 and 19 of 32 stations, respectively) indicate regional improvements in nutrient-related water-quality conditions. Most of the recent trends detected for total phosphorus were upward (17 of 83 stations), indicating possible reversals to the long-term improvements. Concentrations of nutrients in many streams persist at levels that are likely to affect aquatic habitat adversely and promote freshwater or coastal eutrophication. Recent trends for modeled instream concentrations, and modeled reference concentrations, were evaluated relative to ecoregion-based nutrient criteria proposed by the U.S. Environmental Protection Agency. Instream concentrations of total nitrogen and total phosphorus persist at levels higher than proposed criteria at more than one-third and about one-half, respectively, of the 46 stations analyzed. Long-term trends in nutrient loads were primarily downward, with downward trends in total nitrogen and total phosphorus loads detected at 12 and 17 of 32 stations, respectively. Upward trends were rare, with one upward trend for total nitrogen loads and none for total phosphorus. Trends in loads of nitrite-plus-nitrate nitrogen included 7 upward and 8 downward trends among 32 stations. Downward trends in loads of ammonia nitrogen and total Kjeldahl nitrogen were detected at all six stations evaluated. Long-term downward trends detected in four of the five largest drainage basins evaluated include: total nitrogen loads for the Connecticut, Delaware, and James Rivers; total Kjeldahl nitrogen and ammonia nitrogen loads for the Susquehanna River; ammonia nitrogen and nitrite-plus-nitrate nitrogen loads for the James River; and total phosphorus loads for the Connecticut and Delaware Rivers. No trends in load were detected for the Potomac River. Nutrient yields were evaluated relative to the extent of land development in 93 drainage basins. The undeveloped land-use category included forested drainage basins with undeveloped land ranging from 75 to 100 percent of basin area. Median total nitrogen yields for the 27 undeveloped drainage basins evaluated, including 9 basins evaluated in a national NAWQA study, ranged from 290 to 4,800 pounds per square mile per year (lb/mi2/yr). Total nitrogen yields even in the most pristine drainage basins may be elevated relative to natural conditions, because of high rates of atmospheric deposition of nitrogen in parts of the northeastern United States. Median total phosphorus yields ranged from 12 to 330 lb/mi2/yr for the 26 undeveloped basins evaluated. The undeveloped category includes some large drainage basins with point-source discharges and small percentages of developed land; in these basins, streamflow from undeveloped headwater areas dilutes streamflow in more urbanized reaches, and dampens but does not eliminate the point-source "signal" of higher nutrient loads. Median total nitrogen yields generally do not exceed 1,700 lb/mi2/yr, and median total phosphorus yields generally do not exceed 100 lb/mi2/yr, in the drainage basins that are least affected by human land-use and waste-disposal practices. Agricultural and urban land use has increased nutrient yields substantially relative to undeveloped drainage basins. Median total nitrogen yields for 24 agricultural basins ranged from 1,700 to 26,000 lb/mi2/yr, and median total phosphorus yields ranged from 94 to 1,000 lb/mi2/yr. The maximum estimated total nitrogen and total phosphorus yields, 32,000 and 16,000 lb/mi2/yr, respectively, for all stations in the region were in small (less than 50 square miles (mi2)) agricultural drainage basins. Median total nitrogen yields ranged from 1,400 to 17,000 lb/mi2/yr in 26 urbanized drainage basins, and median total phosphorus yields ranged from 43 to 1,900 lb/mi2/yr. Urbanized drainage basins with the highest nutrient yields are generally small (less than 300 mi2) and are drained by streams that receive major point-source discharges. Instream nutrient loads were evaluated relative to loads from point-source discharges in four drainage basins: the Quinebaug River Basin in Connecticut, Massachusetts, and Rhode Island; the Raritan River Basin in New Jersey; the Patuxent River Basin in Maryland; and the James River Basin in Virginia. Long-term downward trends in nutrient loads, coupled with similar trends in flow-adjusted nutrient concentrations, indicate long-term reductions in the delivery of most nutrients to these streams. However, the absence of recent downward trends in load for most nutrients, coupled with instream concentrations that exceed proposed nutrient criteria in several of these waste-receiving streams, indicates that challenges remain in reducing delivery of nutrients to streams from point sources. During dry years, the total nutrient load from point sources in some of the drainage basins approached or equaled the nutrient load transported by the stream.

  18. Characterization of carbohydrate-protein matrices for nutrient delivery.

    PubMed

    Zhou, Yankun; Roos, Yrjö H

    2011-05-01

    Amorphous carbohydrates may show glass transition and crystallization as a result of thermal or water plasticization. Proteins often affect the state transitions of carbohydrates in carbohydrate-protein systems. Water sorption behavior and effects of water on glass transition and crystallization in freeze-dried lactose, trehalose, lactose-casein (3: 1), lactose-soy protein isolate (3:1), trehalose-casein (3:1), and trehalose-soy protein isolate (3:1) systems were studied. Water sorption was determined gravimetrically as a function of time, and Brunauer-Emmett-Teller (BET) and Guggenheim-Anderson-de Boer (GAB) models were fitted to the experimental data. Glass transition temperature (T(g)) and instant crystallization temperature (T(ic)) in anhydrous and water plasticized systems were measured using differential scanning calorimetry (DSC). The Gordon-Taylor equation was used to model water content dependence of the T(g) values. The critical water content and water activity (a(w)) at 24 °C were calculated and crystallization of lactose and trehalose in the systems was followed at and above 0.54 a(w). Carbohydrate-protein systems showed higher amounts of sorbed water and less rapid sugar crystallization than pure sugars. A greater sugar crystallization delay was found in carbohydrate-casein systems than in carbohydrate-soy protein isolate systems. The T(g) and T(ic) values decreased with increasing water content and a(w). However, higher T(ic) values for lactose-protein systems were found than for lactose at the same a(w). Trehalose showed lower T(ic) value than lactose at 0.44 a(w) but no instant crystallization was measured below 0.44 a(w). State diagrams for each system are useful in selecting processing parameters and storage conditions in nutrient delivery applications. © 2011 Institute of Food Technologists®

  19. Nutrient fluxes and stoichiometry in a large impounded river-bay system

    NASA Astrophysics Data System (ADS)

    Klump, J. V.; Waples, J. T.; Able, L. M.; Anderson, P. D.; Weckerly, K.; Szmania, D. C.

    2003-04-01

    Reservoir-induced aging of continental runoff has been shown to an anthropogenically induced global phenomenon with estimates that the mean age of river water reaching the coastal ocean has likely tripled historically. This aging is hypothesized to have a significant biogeochemical impact on land-margin systems by altering flow regimes, net water balances and residence times, reaeration of surface waters, carbon cycling processes, and sediment storage and transport. The Fox-Wolf watershed system contains more than 20 reservoirs, impoundments and lakes on the main stems of the two principal rivers that feed Green Bay and Lake Michigan. Consequently, this hydrologic system can be conceived as functioning as a series of linked biogeochemical reactors which retard flow, retain particles, significantly attenuate the flux of materials into sequential downstream "pools", and both process and repackage nutrients via tightly coupled benthic-pelagic biotic interactions. This successional transformation process results in a poorly understood delivery of nutrients, soils and contaminants from upstream sources to downstream receptors in Green Bay and ultimately -- Lake Michigan. Nutrient reprocessing (defined as the sum of all processes affecting nutrients, i.e. fixation, remineralization, repackaging, sedimentation, etc.) within each pool is hypothesized to be primarily a function of: (1) particle-solute and hydraulic residence times, (2) the quality and quantity of inputs, and (3) the food web structure. Overlaid on these dynamics are very strong seasonal forcing factors, including annual temperature cycles that induce order of magnitude variations in temperature dependent reaction rates, and winter ice cover on the upper pool lakes, reservoirs and Green Bay, that halts run off from the land and reduces within-basin mixing. These short term and seasonal loading dynamics result in considerable temporal stochasticity in the capacity of the biotic component of the ecosystem to assimilate, transform, and attenuate the flux of materials through the land margin system. We report here preliminary results on the nature of elemental riverine fluxes (e.g. carbon, nitrogen, phosphorus, silica), the shift in their composition and stoichiometry as these materials move downstream, and on the role of impoundments as nutrient traps.

  20. Nutrients affecting gastric barrier.

    PubMed

    Gasbarrini, Antonio; D'Aversa, Francesca; Di Rienzo, Teresa; Franceschi, Francesco

    2014-01-01

    The gastric barrier could be considered an active tissue involved in many synthetic and metabolic functions, as the immunological defense, by activating mucosal immune system. Barrier integrity results from a balance between protective and aggressive endogenous factors and from their interaction with exogenous factors (steroidal or nonsteroidal anti-inflammatory drugs, dietary nitrates, nitrites and/or NaCl, stress, Helicobacter pylori infection, food allergens and contaminants, metals, chemicals, radiation, smoking and alcohol intake). Nutrients represent the most important exogenous factors affecting gastric barrier because of the impact on people's everyday life. We report evidence from the literature about nutrients affecting gastric barrier and we investigate the possible effect that nutrients can play to determining or maintaining a gastric barrier dysfunction. © 2014 S. Karger AG, Basel.

  1. High-fat diet affects gut nutrients transporters in hypo and hyperthyroid mice by PPAR-a independent mechanism.

    PubMed

    Losacco, Mariana Cerqueira; de Almeida, Carolina Fernanda Theodora; Hijo, Andressa Harumi Torelli; Bargi-Souza, Paula; Gama, Patricia; Nunes, Maria Tereza; Goulart-Silva, Francemilson

    2018-06-01

    High fat diet consumes and thyroid hormones (THs) disorders may affect nutrients metabolism, but their impact on the absorptive epithelium, the first place of nutrients access, remains unknown. Our aim was to evaluate the intestinal morphology and nutrients transporters content in mice fed standard (LFD) or high fat (HFD) diets in hypo or hyperthyroidism-induced condition. C57BL/6 male mice fed LFD or HFD diets for 12 weeks, followed by saline, PTU (antithyroid drug) or T3 treatment up to 30 days. The mice were euthanized and proximal intestine was removed to study GLUT2, GLUT5, PEPT1, FAT-CD36, FATP4, NPC1L1 and NHE3 distribution by Western blotting. Since PPAR-a is activated by fatty acids, which is abundant in the HFD, we also evaluated whether PPAR-a affects nutrients transporters. Thus, mice were treated with fenofibrate, a PPAR-a agonist. HFD decreased GLUT2, PEPT1, FAT-CD6 and NPC1L1, but increased NHE3, while GLUT5 and FATP4 remained unaltered. THs did not alter distribution of nutrients transporters neither in LFD nor in HFD groups, but they increased villi length and depth crypt in LFD and HFD, respectively. Fenofibrate did not affect content of nutrients transporters, excluding PPAR-a involvement on the HFD-induced changes. We assume that chronic HFD consumption reduced most of the nutrients transporters content in the small intestine of mice, which might limit the entrance of nutrients and gain weight. Since NHE3 promotes sodium absorption, and it was increased in HFD group, this finding could contribute to explain the hypertension observed in obesity. Copyright © 2018 Elsevier Inc. All rights reserved.

  2. Assessment of freshwater ecosystem services in the Beas River Basin, Himalayas region, India

    NASA Astrophysics Data System (ADS)

    Ncube, Sikhululekile; Beevers, Lindsay; Adeloye, Adebayo J.; Visser, Annie

    2018-06-01

    River systems provide a diverse range of ecosystem services, examples include: flood regulation (regulating), fish (provisioning), nutrient cycling (supporting) and recreation (cultural). Developing water resources through the construction of dams (hydropower or irrigation) can enhance the delivery of provisioning ecosystem services. However, these hydrologic alterations result in reductions in less tangible regulating, cultural and supporting ecosystem services. This study seeks to understand how multiple impoundments, abstractions and transfers within the upper Beas River Basin, Western Himalayas, India, are affecting the delivery of supporting ecosystem services. Whilst approaches for assessing supporting ecosystem services are under development, the immediate aim of this paper is to set out a framework for their quantification, using the macroinvertebrate index Lotic-Invertebrate Index for Flow Evaluation (LIFE). LIFE is a weighted measure of the flow velocity preferences of the macroinvertebrate community. Flow records from multiple gauging stations within the basin were used to investigate flow variability at seasonal, inter-annual and decadal time scales. The findings show that both mean monthly and seasonal cumulative flows have decreased over time in the Beas River Basin. A positive hydroecological relationship between LIFE and flow was also identified, indicative of macroinvertebrate response to seasonal changes in the flow regime. For example, high LIFE scores (7.7-9.3) in the winter and summer seasons indicate an abundance of macroinvertebrates with a preference for high flows; this represents a high potential for instream supporting ecosystem services delivery. However, further analysis is required to understand these hydroecological interactions in the study basin and the impact on instream supporting ecosystem services delivery.

  3. Effects of productivity, consumers, competitors, and El Nino events on food chain patterns in a rocky intertidal community

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wootton, J.T.; Pfister, C.A.; Paine, R.T.

    We experimentally manipulated nutrient input to a rocky intertidal community, using nutrient-diffusing flowerpots, to determine (i) whether nutrients limited intertidal productivity, (ii) how a large-scale oceanographic disturbance (an El Nino event) affected patterns of nutrient limitation, (iii) the relative impacts of molluscan grazers and nutrient limitation, and (iv) if responses to experimental nutrient addition among trophic levels were more consistent with prey-dependent or ratio-dependent food chain models. Nutrients measurably increased the abundance of micrograzers (amphipods and chironomid larvae), but not algal biomass, during the summer of an El Nino years and during the autumn of an El Nino year. Addingmore » nutrients did not affect food chain stability as assessed by temporal variation in algal biomass and micrograzer abundance. Large molluscan grazers caused large reductions in micrograzers and smaller reductions in algae, indicating consistent consumer effects. The results demonstrate that in this intertidal community, nutrient limitation can occur under conditions of nutrient stress, that top-down grazing effects are typically stronger than bottom-up nutrient effects, and that prey-dependent models are more appropriate than ratio-dependent models. 40 refs., 1 fig., 1 tab.« less

  4. Drug-nutrient interactions.

    PubMed

    Thomas, J A

    1995-10-01

    Nutrition status plays a significant role in a drug's pharmacodynamics. Some disease states and other special conditions affect nutrient status and a drug's therapeutic efficacy. Many classes of drugs, including antimicrobials, hypoglycemics, and hypocholesterolemic agents, can be affected by the presence of food, with the geriatric patient particularly at risk. While a drug's pharmacokinetic profile can usually be predicted, it can be modified by nutrients and by certain pathophysiologic conditions, including aging, hepatic dysfunction, and micronutrients.

  5. Reviews and syntheses: Effects of permafrost thaw on Arctic aquatic ecosystems

    NASA Astrophysics Data System (ADS)

    Vonk, J. E.; Tank, S. E.; Bowden, W. B.; Laurion, I.; Vincent, W. F.; Alekseychik, P.; Amyot, M.; Billet, M. F.; Canário, J.; Cory, R. M.; Deshpande, B. N.; Helbig, M.; Jammet, M.; Karlsson, J.; Larouche, J.; MacMillan, G.; Rautio, M.; Anthony, K. M. Walter; Wickland, K. P.

    2015-12-01

    The Arctic is a water-rich region, with freshwater systems covering about 16 % of the northern permafrost landscape. Permafrost thaw creates new freshwater ecosystems, while at the same time modifying the existing lakes, streams, and rivers that are impacted by thaw. Here, we describe the current state of knowledge regarding how permafrost thaw affects lentic (still) and lotic (moving) systems, exploring the effects of both thermokarst (thawing and collapse of ice-rich permafrost) and deepening of the active layer (the surface soil layer that thaws and refreezes each year). Within thermokarst, we further differentiate between the effects of thermokarst in lowland areas vs. that on hillslopes. For almost all of the processes that we explore, the effects of thaw vary regionally, and between lake and stream systems. Much of this regional variation is caused by differences in ground ice content, topography, soil type, and permafrost coverage. Together, these modifying factors determine (i) the degree to which permafrost thaw manifests as thermokarst, (ii) whether thermokarst leads to slumping or the formation of thermokarst lakes, and (iii) the manner in which constituent delivery to freshwater systems is altered by thaw. Differences in thaw-enabled constituent delivery can be considerable, with these modifying factors determining, for example, the balance between delivery of particulate vs. dissolved constituents, and inorganic vs. organic materials. Changes in the composition of thaw-impacted waters, coupled with changes in lake morphology, can strongly affect the physical and optical properties of thermokarst lakes. The ecology of thaw-impacted lakes and streams is also likely to change; these systems have unique microbiological communities, and show differences in respiration, primary production, and food web structure that are largely driven by differences in sediment, dissolved organic matter, and nutrient delivery. The degree to which thaw enables the delivery of dissolved vs. particulate organic matter, coupled with the composition of that organic matter and the morphology and stratification characteristics of recipient systems will play an important role in determining the balance between the release of organic matter as greenhouse gases (CO2 and CH4), its burial in sediments, and its loss downstream. The magnitude of thaw impacts on northern aquatic ecosystems is increasing, as is the prevalence of thaw-impacted lakes and streams. There is therefore an urgent need to quantify how permafrost thaw is affecting aquatic ecosystems across diverse Arctic landscapes, and the implications of this change for further climate warming.

  6. Nitrogen and phosphorus fluxes from watersheds of the northeast U.S. from 1930 to 2000: Role of anthropogenic nutrient inputs, infrastructure, and runoff

    NASA Astrophysics Data System (ADS)

    Hale, Rebecca L.; Grimm, Nancy B.; Vörösmarty, Charles J.; Fekete, Balazs

    2015-03-01

    An ongoing challenge for society is to harness the benefits of nutrients, nitrogen (N) and phosphorus (P), while minimizing their negative effects on ecosystems. While there is a good understanding of the mechanisms of nutrient delivery at small scales, it is unknown how nutrient transport and processing scale up to larger watersheds and whole regions over long time periods. We used a model that incorporates nutrient inputs to watersheds, hydrology, and infrastructure (sewers, wastewater treatment plants, and reservoirs) to reconstruct historic nutrient yields for the northeastern U.S. from 1930 to 2002. Over the study period, yields of nutrients increased significantly from some watersheds and decreased in others. As a result, at the regional scale, the total yield of N and P from the region did not change significantly. Temporal variation in regional N and P yields was correlated with runoff coefficient, but not with nutrient inputs. Spatial patterns of N and P yields were best predicted by nutrient inputs, but the correlation between inputs and yields across watersheds decreased over the study period. The effect of infrastructure on yields was minimal relative to the importance of soils and rivers. However, infrastructure appeared to alter the relationships between inputs and yields. The role of infrastructure changed over time and was important in creating spatial and temporal heterogeneity in nutrient input-yield relationships.

  7. A method to quantify and value floodplain sediment and nutrient retention ecosystem services

    USGS Publications Warehouse

    Hopkins, Kristina G.; Noe, Gregory; Franco, Fabiano; Pindilli, Emily J.; Gordon, Stephanie; Metes, Marina J.; Claggett, Peter; Gellis, Allen; Hupp, Cliff R.; Hogan, Dianna

    2018-01-01

    Floodplains provide critical ecosystem services to local and downstream communities by retaining floodwaters, sediments, and nutrients. The dynamic nature of floodplains is such that these areas can both accumulate sediment and nutrients through deposition, and export material downstream through erosion. Therefore, estimating floodplain sediment and nutrient retention should consider the net flux of both depositional and erosive processes. An ecosystem services framework was used to quantify and value the sediment and nutrient ecosystem service provided by floodplains in the Difficult Run watershed, a small (151 km2) suburban watershed located in the Piedmont of Virginia (USA). A sediment balance was developed for Difficult Run and two nested watersheds. The balance included upland sediment delivery to streams, stream bank flux, floodplain flux, and stream load. Upland sediment delivery was estimated using geospatial datasets and a modified Revised Universal Soil Loss Equation. Predictive models were developed to extrapolate field measurements of the flux of sediment, sediment-bound nitrogen (N), and sediment-bound phosphorus (P) from stream banks and floodplains to 3232 delineated stream segments in the study area. A replacement cost approach was used to estimate the economic value of the sediment and nutrient retention ecosystem service based on estimated net stream bank and floodplain flux of sediment-bound N for all streams in the study area. Results indicated the net fluvial fluxes of sediment, sediment-bound N, and sediment-bound P were −10,439 Mg yr−1 (net export), 57,300 kg-N yr−1(net trapping), and 98 kg-P yr−1(net trapping), respectively. For sediment, floodplain retention was offset by substantial losses from stream bank erosion, particularly in headwater catchments, resulting in a net export of sediment. Nutrient retention in the floodplain exceeded that lost through stream bank erosion resulting in net retention of nutrients (TN and TP). Using a conservative cost estimate of $12.69 (USD) per kilogram of nitrogen, derived from wastewater treatment costs, the estimated annual value for sediment and nutrient retention on Difficult Run floodplains was $727,226 ± 194,220 USD/yr. Values and differences in floodplain nitrogen retention among stream reaches can be used to target areas for floodplain conservation and stream restoration. The methods presented are scalable and transferable to other areas if appropriate datasets are available for validation.

  8. Monitoring total mixed rations and feed delivery systems.

    PubMed

    Oelberg, Thomas J; Stone, William

    2014-11-01

    This article is intended to give practitioners a method to evaluate total mixed ration (TMR) consistency and to give them practical solutions to improve TMR consistency that will improve cattle performance and health. Practitioners will learn how to manage the variation in moisture and nutrients that exists in haylage and corn silage piles and in bales of hay, and methods to reduce variation in the TMR mixing and delivery process. Copyright © 2014 Elsevier Inc. All rights reserved.

  9. Equatorial Pacific peak in biological production regulated by nutrient and upwelling during the late Pliocene/early Pleistocene cooling

    NASA Astrophysics Data System (ADS)

    Etourneau, J.; Robinson, R. S.; Martinez, P.; Schneider, R.

    2013-03-01

    The largest increase in export production in the eastern Pacific of the last 5.3 Myr (million years) occurred between 2.2 and 1.6 Myr, a time of major climatic and oceanographic reorganization in the region. Here, we investigate the causes of this event using reconstructions of export production, nutrient supply and oceanic conditions across the Pliocene-Pleistocene in the eastern equatorial Pacific (EEP) for the last 3.2 Myr. Our results indicate that the export production peak corresponds to a cold interval marked by high nutrient supply relative to consumption, as revealed by the low bulk sedimentary 15N/14N (δ15N) and alkenone-derived sea surface temperature (SST) values. This ~ 0.6 million years long episode of enhanced delivery of nutrients to the surface of the EEP was predominantly initiated through the upwelling of nutrient-enriched water sourced in high latitudes. In addition, this phenomenon was likely promoted by the regional intensification of upwelling in response to the development of intense Walker and Hadley atmospheric circulations. Increased nutrient consumption in the polar oceans and enhanced denitrification in the equatorial regions restrained nutrient supply and availability and terminated the high export production event.

  10. A mathematical model of water and nutrient transport in xylem vessels of a wheat plant.

    PubMed

    Payvandi, S; Daly, K R; Jones, D L; Talboys, P; Zygalakis, K C; Roose, T

    2014-03-01

    At a time of increasing global demand for food, dwindling land and resources, and escalating pressures from climate change, the farming industry is undergoing financial strain, with a need to improve efficiency and crop yields. In order to improve efficiencies in farming, and in fertiliser usage in particular, understanding must be gained of the fertiliser-to-crop-yield pathway. We model one aspect of this pathway; the transport of nutrients within the vascular tissues of a crop plant from roots to leaves. We present a mathematical model of the transport of nutrients within the xylem vessels in response to the evapotranspiration of water. We determine seven different classes of flow, including positive unidirectional flow, which is optimal for nutrient transport from the roots to the leaves; and root multidirectional flow, which is similar to the hydraulic lift process observed in plants. We also investigate the effect of diffusion on nutrient transport and find that diffusion can be significant at the vessel termini especially if there is an axial efflux of nutrient, and at night when transpiration is minimal. Models such as these can then be coupled to whole-plant models to be used for optimisation of nutrient delivery scenarios.

  11. Viviparous placentotrophy in reptiles and the parent-offspring conflict.

    PubMed

    Blackburn, Daniel G

    2015-09-01

    In placentotrophic viviparous reptiles, pregnant females deliver nutrients to their developing fetuses by diverse morphological specializations that reflect independent evolutionary origins. A survey of these specializations reveals a major emphasis on histotrophy (uterine secretion and fetal absorption) rather than hemotrophy (transfer between maternal and fetal blood streams). Of available hypotheses for the prevalence of histotrophic transfer, the most promising derives insights from the theoretical parent-offspring conflict over nutrient investment. I suggest that histotrophy gives pregnant females greater control over nutrient synthesis, storage, and delivery than hemotrophic transfer, reflecting maternal preeminence in any potential parent-offspring competition over nutrient investment. One lizard species shows invasive ovo-implantation and direct contact between fetal tissues and maternal blood vessels, potentially conferring control over nutrient transfer to the embryo. Future research on squamates will benefit from application of parent-offspring conflict theory to the transition from incipient to substantial matrotrophy, as well as by testing theory-derived predictions on both facultatively and highly placentotrophic forms. © 2015 Wiley Periodicals, Inc.

  12. The Plant Research Unit: An International Space Station Habitat

    NASA Technical Reports Server (NTRS)

    Morrow, Robert; Reiss-Bubenheim, Debra; Schaefer, Ronald L.

    2003-01-01

    The Plant Research Unit (PRU) is one of six life science habitats being developed as part of the Space Station Biological Research Program. The PRU is designed for experiments in microgravity and will utilize the ISS Centrifuge Facility to provide gravity levels between microgravity and 29. The PRU will provide and control all aspects of a plant s needs in a nearly closed system. In other words, the shoot and root environments will not be open to the astronaut s environment except for experiment maintenance such as planting, harvesting and plant sampling. This also means that all lighting, temperature and humidity control, "watering," and air filtering and cleaning .must be done within strict limitations of volume, weight, power, and crew time while at the same time providing a very high level of reliability and a service life in excess of 10 years. The PRU will contain two plant chambers 31.5 cm tall, each with independent control of temperature, humidity, light level and photoperiod, CO2 level, nutrient and water delivery, and video and data acquisition. The PRU is currently in the preliminary design phase and a number of subsystem components have been prototyped for testing, including the temperature and humidity control systems, the plant chambers, the LED lighting system, the atmospheric control system and a variety of nutrient delivery systems. The LED prototype provides independent feedback control of 5 separate spectral bands and variable output between 0 and 1000 micro-mol sq m/sec. The water and nutrient delivery system (WNDS) prototypes have been used to test particulate based, thin film, and gel-based WNDS configurations.

  13. Operational Evaluation of VEGGIE Food Production System in the Habitat Demonstration Unit

    NASA Technical Reports Server (NTRS)

    Stutte, Gary W.; Newsham, Gerard; Morrow, Robert M.; Wheeler, Raymond M.

    2011-01-01

    The 2010 Desert Research and Technology Studies (DRATS) of the VEGGIE Food Production System in the Habitat Demonstration Unit (HDU) Pressurized Excursion Module (PEM) was the first operational evaluation of salad crop production technology in a NASA analog test. A systematic evaluation of rooting media and nutrient delivery systems were evaluated for three lettuce cultivars that have shown promise as candidates for a surface based food production system. The VEGGIE nutrient delivery system worked well, was able to be maintained by multiple operators with a minimum of training, and supported excellent lettuce growth for the duration of the test. A Hazard Analysis and Critical Control Point (HACCP) evaluation was performed using ProSan(tm) as sanitation agent prior to consumption was approved, and the crew was allowed to consume the lettuce grown using the VEGGIE light cap and gravity based nutrient delivery system at the completion of the 14-day DRAT field test. The DRAT field test validated the crew operations; Growth of all lettuce cultivars was excellent. The operational DRAT field testing in the HDU identified light quality issues related to morphology and pigment development that will need to be addressed through additional testing. Feedback from the crew, ground support personnel, and human factors leads was uniformly positive on the psychological value of having the crop production system in the excursion module. A number of areas have been identified for future work, to minimize the "footprint" of the Food Production system through creative use of unused wall and floor space in the unit.

  14. Operation Evaluation of the VEGGIE Food Production System in the Habitat Demonstration Unit

    NASA Technical Reports Server (NTRS)

    Stutte, Gary W.; Newsham, Gerard; Morrow, Robert M.; Wheeler, Raymond M.

    2011-01-01

    The 2010 Desert Research and Technology Studies (DRATS) of the VEGGIE Food Production System in the Habitat Demonstration Unit (HDU) Pressurized Excursion Module (PEM) was the first operational evaluation of salad crop production technology in a NASA analog test. A systematic evaluation of rooting media and nutrient delivery systems were evaluated for three lettuce cultivars that have shown promise as candidates for a surface based food production system. The VEGGIE nutrient delivery system worked well, was able to be maintained by multiple operators with a minimum of training, and supported excellent lettuce growth for the duration of the test. A Hazard Analysis and Critical Control Point (HACCP) evaluation was performed using ProSantm as sanitation agent prior to consumption was approved, and the crew was allowed to consume the lettuce grown using the VEGGIE light cap and gravity based nutrient delivery system at the completion of the 14-day DRAT field test. The DRAT field test validated the crew operations; Growth of all lettuce cultivars was excellent. The operational DRAT field testing in the HDU identified light quality issues related to morphology and pigment development that will need to be addressed through additional testing. Feedback from the crew, ground support personnel, and human factors leads was uniformly positive on the psychological value of having the crop production system in the excursion module. A number of areas have been identified for future work, to minimize the "footprint" of the Food Production system through creative use of unused wall and floor space in the unit.

  15. Controlled-release fertilizer composition substantially coated with an impermeable layer

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ankeny, Mark

    2016-03-29

    A controlled-release fertilizer composition is provided that is substantially coated with an impermeable layer. The fertilizer composition may further include one or more hollow sections to allow for root penetration and efficient delivery of nutrients.

  16. vEmbryo In Silico Models: Predicting Vascular Developmental Toxicity

    EPA Science Inventory

    The cardiovascular system is the first to function in the vertebrate embryo, reflecting the critical need for nutrient delivery and waste removal during organogenesis. Blood vessel development occurs by complex interacting signaling networks, including extra-cellular matrix remod...

  17. A prospective survey of nutritional support practices in intensive care unit patients: what is prescribed? What is delivered?

    PubMed

    De Jonghe, B; Appere-De-Vechi, C; Fournier, M; Tran, B; Merrer, J; Melchior, J C; Outin, H

    2001-01-01

    To assess the amount of nutrients delivered, prescribed, and required for critically ill patients and to identify the reasons for discrepancies between prescriptions and requirements and between prescriptions and actual delivery of nutrition. Prospective cohort study. Twelve-bed medical intensive care unit in a university-affiliated general hospital. Fifty-one consecutive patients, receiving nutritional support either enterally or intravenously for > or = 2 days. We followed patients for the first 14 days of nutritional delivery. The amount of calories prescribed and the amount actually delivered were recorded daily and compared with the theoretical energy requirements. A combined regimen of enteral and parenteral nutrition was administered on 58% of the 484 nutrition days analyzed, and 63.5% of total caloric intake was delivered enterally. Seventy-eight percent of the mean caloric amount required was prescribed, and 71% was effectively delivered. The amount of calories actually delivered compared with the amount prescribed was significantly lower in enteral than in parenteral administration (86.8% vs. 112.4%, p < .001). Discrepancies between prescription and delivery of enterally administered nutrients were attributable to interruptions caused by digestive intolerance (27.7%, mean daily wasted volume 641 mL), airway management (30.8%, wasted volume 745 mL), and diagnostic procedures (26.6%, wasted volume 567 mL). Factors significantly associated with a low prescription rate of nutritional support were the administration of vasoactive drugs, central venous catheterization, and the need for extrarenal replacement. An inadequate delivery of enteral nutrition and a low rate of nutrition prescription resulted in low caloric intake in our intensive care unit patients. A large volume of enterally administered nutrients was wasted because of inadequate timing in stopping and restarting enteral feeding. The inverse correlation between the prescription rate of nutrition and the intensity of care required suggests that physicians need to pay more attention to providing appropriate nutritional support for the most severely ill patients.

  18. The Impact of Extreme Flooding on Mussel and Microbial Nutrient Dynamics at the Water-Sediment Interface

    NASA Astrophysics Data System (ADS)

    Bril, J.; Just, C. L.; Newton, T.; Young, N.; Parkin, G.

    2009-12-01

    Labeled by the National Academy of Engineering as one of fourteen grand challenges for engineering, the management of the nitrogen cycle has become an increasingly difficult obstacle for sustainable development. In an effort to improve nitrogen cycle management practices, we are attempting to expand on the limited scientific knowledge of how aquatic environments are affected by increasing human- and climate-induced changes. To accomplish this, we are using freshwater mussels as a sentinel species to indicate how natural processes within large river systems may be altered by human activity. Freshwater mussels have been referred to as ‘ecosystem engineers’ because they exert control over food resources and alter habitats for other organisms. Also, mussels and bacteria play a major role in nutrient cycling in large river systems by cycling nutrients taken up by phytoplankton and zooplankton. Under ‘normal’ environmental conditions, mussels appear to process nitrogen more rapidly than denitrifying bacteria. However, substantial deposition of carbon-rich sediment resulting from extreme flooding may increase bacterial nitrogen cycling rates and subsequently alter overall denitrification rates. We hypothesize that intense depositions of particulate matter from recent extreme floods in the Upper Mississippi River Basin (UMRB) have altered the freshwater mussel and microbial food webs through physical and chemical means. This work will be done in a 1200-m reach of the UMRB near Buffalo, Iowa. The reach contains a healthy and diverse assemblage of freshwater mussels. A historic flood event during May-July 2008 coincided with intense spring cultivation and nutrient application activities in the heavily farmed landscape of the Upper Midwest and resulted in a significant pulse of agricultural contaminants to the UMRB. This led scientists to predict an almost unprecedented delivery of sediment and nutrients to the mussel bed, the broader Mississippi River, and ultimately to the Gulf of Mexico. We will correlate the rate of nitrogen removal by mussels to the concentrations of organic carbon that may have been deposited during the flood. Initial studies suggest that the highest amount of total organic carbon exists in areas of fine sediments within the mussel bed. Additionally, bacterial nitrate reduction studies indicate that significantly higher rates of denitrification occur in areas of high organic content. Increased availability of organic carbon may affect the rate that mussels process nitrogen. In field studies, mussel densities are generally greater in areas of coarser sediments (thus, less carbon and less bacterial nutrient processing). We are currently working to determine the role of organic carbon availability on denitrification in a laboratory system containing mussels and bacteria. We also hope to couple sediment grain size with organic carbon to compare organic carbon content pre- and post-flood.

  19. Is groundwater discharge the dominant source of nutrients to Alabama estuaries and will it keep impacting these waters for the foreseeable future?

    NASA Astrophysics Data System (ADS)

    Mortazavi, B.; Domangue, R.; Kleinhuizen, A.; Tatariw, C.

    2017-12-01

    Land use change and population growth are dominant factors impacting coastal waters. Populations in Alabama coastal counties have increased by several folds since the 1950s and a large fraction of the farmed land are now being used for growing sod requiring large amounts of fertilizers. Concurrent with these changes, marshes bordering Mobile Bay have been disappearing such that they now only cover 50% of their areal extent compared to the 1780s. These changes in land use and coastal geomorphology, as well as the population growth ultimately result in larger delivery of nutrients either through runoff or groundwater discharge to the coastal waters. The Mobile Bay estuary in Alabama is bordered with several subestuaries and a coastal lagoon. Our investigations suggest that the large inputs of nutrients through river discharge in Weeks Bay (140 g N m-2 yr-1) and groundwater discharge in Little Lagoon (300 g N m-2 yr-1) by far dominate inputs of N to the water column and exceed N input, for example, from benthic regeneration, by an order of magnitude. Furthermore, the capacity for N removal through denitrification in these systems is low and instead nitrogen is retained through dissimilatory nitrate reduction to ammonium at a rate that exceed denitrification by an order of magnitude. Our measurements also suggest that once marshes are transformed to subtidal unvegetated sediments rates of nitrogen removal by denitrification decline four folds. Excessive inputs of nitrogen and the high efficiency with which nitrogen is retained in these systems is impacting the foodweb and harmful algal blooms and fish kills are reoccurring events. While changes in agricultural practices and reconstruction of marshes can potentially reduce the delivery of N or enhance N removal by denitrification, nutrient inputs through groundwater discharge are going to impact these estuaries for the foreseeable future. Our capacity to construct nutrient budgets and to predict the trajectory of ecosystem changes will therefore depend greatly on accurate knowledge of groundwater discharge to these systems. Quantifying the magnitudes of groundwater derived nutrients and the fate of these nutrients in nearshore systems requires concerted efforts amongst hydrologists, biogeochemists, and ecologists.

  20. Mechanisms linking employee affective delivery and customer behavioral intentions.

    PubMed

    Tsai, Wei-Chi; Huang, Yin-Mei

    2002-10-01

    Past empirical evidence has indicated that employee affective delivery can influence customer reactions (e.g., customer satisfaction, service quality evaluation). This study extends previous research by empirically examining mediating processes underlying the relationship between employee affective delivery and customer behavioral intentions. Data were collected from 352 employee-customer pairs in 169 retail shoe stores in Taiwan. Results showed that the influence of employee affective delivery on customers' willingness to return to the store and pass positive comments to friends was indirect through the mediating processes of customer in-store positive moods and perceived friendliness. The study also indicated that employee affective delivery influences customers' time spent in store, which, in turn, influences customer behavioral intentions.

  1. Nutrients affecting brain composition and behavior

    NASA Technical Reports Server (NTRS)

    Wurtman, R. J.

    1987-01-01

    This review examines the changes in brain composition and in various brain functions, including behavior, that can follow the ingestion of particular foods or nutrients. It details those that are best understood: the increases in serotonin, catecholamine, or acetylcholine synthesis that can occur subsequent to food-induced increases in brain levels of tryptophan, tyrosine, or choline; it also discusses the various processes that must intervene between the mouth and the synapse, so to speak, in order for a nutrient to affect neurotransmission, and it speculates as to additional brain chemicals that may ultimately be found to be affected by changes in the availability of their nutrient precursors. Because the brain chemicals best known to be nutrient dependent overlap with those thought to underlie the actions of most of the drugs used to treat psychiatric diseases, knowledge of this dependence may help the psychiatrist to understand some of the pathologic processes occurring in his/her patients, particularly those with appetitive symptoms. At the very least, such knowledge should provide the psychiatrist with objective criteria for judging when to take seriously assertions that particular foods or nutrients do indeed affect behavior (e.g., in hyperactive children). If the food can be shown to alter neurotransmitter release, it may be behaviorally-active; however, if it lacks a discernible neurochemical effect, the likelihood that it really alters behavior is small.

  2. Profiling contents of water-soluble metabolites and mineral nutrients to evaluate the effects of pesticides and organic and chemical fertilizers on tomato fruit quality.

    PubMed

    Watanabe, Masami; Ohta, Yuko; Licang, Sun; Motoyama, Naoki; Kikuchi, Jun

    2015-02-15

    In this study, the contents of water-soluble metabolites and mineral nutrients were measured in tomatoes cultured using organic and chemical fertilizers, with or without pesticides. Mineral nutrients and water-soluble metabolites were determined by inductively coupled plasma-atomic emission spectrometry and (1)H nuclear magnetic resonance spectrometry, respectively, and results were analysed by principal components analysis (PCA). The mineral nutrient and water-soluble metabolite profiles differed between organic and chemical fertilizer applications, which accounted for 88.0% and 55.4%, respectively, of the variation. (1)H-(13)C-hetero-nuclear single quantum coherence experiments identified aliphatic protons that contributed to the discrimination of PCA. Pesticide application had little effect on mineral nutrient content (except Fe and P), but affected the correlation between mineral nutrients and metabolites. Differences in the content of mineral nutrients and water-soluble metabolites resulting from different fertilizer and pesticide applications probably affect tomato quality. Copyright © 2014 Elsevier Ltd. All rights reserved.

  3. Partitioning of nutrient net fluxes across the portal-drained viscera in sheep fed twice daily: effect of dietary protein degradability.

    PubMed

    Rémond, Didier; Bernard, Laurence; Savary-Auzeloux, Isabelle; Nozière, Pierre

    2009-08-01

    Extrusion is used to decrease leguminous seed protein degradability in the rumen in order to shift part of the dietary protein digestion towards the small intestine. The effect of such displacement of digestion site on the partitioning of nutrient net fluxes across the gastrointestinal tract was studied using four sheep fitted with catheters and blood-flow probes, allowing measurements across the rumen, the mesenteric-drained viscera (MDV) and the portal-drained viscera (PDV). Two diets containing 34 % of pea seeds were tested in a crossover design. They differed only according to pea treatment: raw pea (RP) or extruded pea (EP) diet. Rumen undegradable protein (RUP) accounted for 23 and 40 % of dietary crude protein for RP and EP diets, respectively. Across the rumen wall, ammonia net flux was lower with EP diet, whereas urea net flux was not different. Across the MDV, free amino acid (FAA) net flux was greater with EP diet, whereas peptide amino acid net flux was not different, accounting for 7 % of the non-protein amino acid net release. From RP to EP diet, PDV net flux of ammonia decreased by 23 %, whereas FAA net release increased by 21 %. The difference in dietary RUP did not affect the PDV net flux of SCFA, 3-hydroxybutyrate, lactate and glucose. In conclusion, the partial shift in pea protein digestion from the rumen to the small intestine did not affect the portal net balance of N, but decreased N loss from the rumen, and increased amino acid intestinal absorption and portal delivery.

  4. KSC-04pd1398

    NASA Image and Video Library

    2004-06-30

    KENNEDY SPACE CENTER, FLA. - Kimberly Beck is a Controlled Biological Systems trainee in the Spaceflight and Life Sciences Training Program. She is helping with growth studies supporting the WONDER (Water Offset Nutrient Delivery Experiment) flight payload, which is investigating hydroponic plant crop production in microgravity.

  5. KSC-04PD-1398

    NASA Technical Reports Server (NTRS)

    2004-01-01

    KENNEDY SPACE CENTER, FLA. Kimberly Beck is a Controlled Biological Systems trainee in the Spaceflight and Life Sciences Training Program. She is helping with growth studies supporting the WONDER (Water Offset Nutrient Delivery Experiment) flight payload, which is investigating hydroponic plant crop production in microgravity.

  6. Monte-Carlo Simulations of Drug Delivery on Biofilms

    NASA Astrophysics Data System (ADS)

    Buldum, Alper; Simpson, Andrew

    2013-03-01

    The focus of this work is on biofilms that grow in the lungs of cystic fibrosis (CF) patients. A discrete model which describes the nutrient and biomass as discrete particles is created. Diffusion of the nutrient, consumption of the nutrient by microbial particles, and growth and decay of microbial particles are simulated using stochastic processes. Our model extends the complexity of the biofilm system by including the conversion and reversion of living bacteria into a hibernated state, known as persister bacteria. Another new contribution is the inclusion of antimicrobial in two forms: an aqueous solution and encapsulated in biodegradable nanoparticles. The bacteria population growth and spatial variation of drugs and their effectiveness are investigated in this work. Supported by NIH

  7. Food prices and poverty negatively affect micronutrient intakes in Guatemala.

    PubMed

    Iannotti, Lora L; Robles, Miguel; Pachón, Helena; Chiarella, Cristina

    2012-08-01

    Limited empirical evidence exists for how economic conditions affect micronutrient nutrition. We hypothesized that increasing poverty and rising food prices would reduce consumption of high-quality "luxury" foods, leading to an increased probability of inadequacy for several nutrients. The 2006 Guatemala National Living Conditions Survey was analyzed. First, energy and nutrient intakes and adequacy levels were calculated. Second, the income-nutrient relationships were investigated by assessing disparities in intakes, determining income-nutrient elasticities, and modeling nutrient intakes by reductions in income. Third, the food price-nutrient relationships were explored through determination of price-nutrient elasticities and modeling 2 price scenarios: an increase in food prices similar in magnitude to the food price crisis of 2007-2008 and a standardized 10% increase across all food groups. Disparities in nutrient intakes were greatest for vitamin B-12 (0.38 concentration index) and vitamin A (0.30 concentration index); these nutrients were highly and positively correlated with income (r = 0.22-0.54; P < 0.05). Although the baseline probability of inadequacy was highest for vitamin B-12 (83%), zinc showed the greatest increase in probability of inadequacy as income was reduced, followed by folate and vitamin A. With rising food prices, zinc intake was most acutely affected under both scenarios (P < 0.05) and folate intake in the poorest quintile (+7 percentage points) under the 10% scenario. Price-nutrient elasticities were highest for vitamin B-12 and the meat, poultry, and fish group (-0.503) and for folate and the legumes group (-0.343). The economic factors of food prices and income differentially influenced micronutrient intakes in Guatemala, notably zinc and folate intakes.

  8. Nutrient Content of Brown Marmorated Stink Bug Eggs and Comparisons Between Experimental Uses

    PubMed Central

    Skillman, Victoria P

    2017-01-01

    Abstract Halyomorpha halys (Stål) (Hemiptera: Pentatomidae), the brown marmorated stink bug (BMSB), has become a major pest. Seven experiments examined the nutrient content of their eggs in the context of female reproductive investment and use for experiments. Among 542 clusters examined, an average egg contained 23.50 ± 0.561 µg lipid, 3.17 ± 0.089 µg glycogen, and 3.08 ± 0.056 µg sugar. Mature eggs within a female’s ovary can make up 61% of her total lipid, 35% of glycogen, and 20% of sugar levels. Eggs obtained from a colony reared on a steady diet are expected to have consistent nutrient content. The age of a parental female only slightly affected the lipid level of oviposited eggs but did not affect glycogen or sugar levels. However, egg nutrient content can differ substantially by the source of the parental females; wild eggs had higher lipid but lower sugar content than colony-produced eggs. Further, the length of time that eggs are frozen influenced egg nutrient content. Freshly laid eggs had higher lipid and lower sugar levels than eggs frozen for 1 or 2 yr. Whether an egg turned grey following removal from cold storage did not affect nutrient content, nor did being frozen within 1 or 3 d of oviposition. The temperature at which eggs were left exposed did not impact egg nutrient content, but glycogen decreased and sugar increased with deployment time. This information combined with how factors affect host selection by natural enemies will help refine future experiments that use BMSB egg clusters.

  9. Factors affecting stream nutrient loads: A synthesis of regional SPARROW model results for the continental United States

    USGS Publications Warehouse

    Preston, Stephen D.; Alexander, Richard B.; Schwarz, Gregory E.; Crawford, Charles G.

    2011-01-01

    We compared the results of 12 recently calibrated regional SPARROW (SPAtially Referenced Regressions On Watershed attributes) models covering most of the continental United States to evaluate the consistency and regional differences in factors affecting stream nutrient loads. The models - 6 for total nitrogen and 6 for total phosphorus - all provide similar levels of prediction accuracy, but those for major river basins in the eastern half of the country were somewhat more accurate. The models simulate long-term mean annual stream nutrient loads as a function of a wide range of known sources and climatic (precipitation, temperature), landscape (e.g., soils, geology), and aquatic factors affecting nutrient fate and transport. The results confirm the dominant effects of urban and agricultural sources on stream nutrient loads nationally and regionally, but reveal considerable spatial variability in the specific types of sources that control water quality. These include regional differences in the relative importance of different types of urban (municipal and industrial point vs. diffuse urban runoff) and agriculture (crop cultivation vs. animal waste) sources, as well as the effects of atmospheric deposition, mining, and background (e.g., soil phosphorus) sources on stream nutrients. Overall, we found that the SPARROW model results provide a consistent set of information for identifying the major sources and environmental factors affecting nutrient fate and transport in United States watersheds at regional and subregional scales. ?? 2011 American Water Resources Association. This article is a U.S. Government work and is in the public domain in the USA.

  10. Applied remediation of petroleum hydrocarbons 3(6)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hinchee, R.E.; Kittel, J.A.; Reisinger, H.J.

    1995-12-31

    This volume provides sound scientific and engineering approaches. Sections of this volume cover bioremediation markets, general technology overviews, and selected case studies of crude oil spills in marine environments, heavy-metal co-contamination, steam injection, nitrate-based bioremediation, land farming, nutrient addition, confined aquifers, anaerobic biodegradation, free-product recovery technologies, bioremediation in low permeability soils and rock, biopile treatment, field-scale studies, oily waste organics as soil amendments, BTEX degradation in a biofilter, surfactant-aided recovery, mass transport in BTEX removal, electron acceptor selection and delivery strategies, and electrokinetic moisture and nutrient control in unsaturated soils.

  11. Engineering solutions of environmental problems in organic waste handling

    NASA Astrophysics Data System (ADS)

    Briukhanov, A. Y.; Vasilev, E. V.; Shalavina, E. V.; Kucheruk, O. N.

    2017-10-01

    This study shows the urgent need to consider modernization of agricultural production in terms of sustainable development, which takes into account environmental implications of intensive technologies in livestock farming. Some science-based approaches are offered to address related environmental challenges. High-end technologies of organic livestock waste processing were substantiated by the feasibility study and nutrient balance calculation. The technologies were assessed on the basis of best available techniques criteria, including measures such as specific capital and operational costs associated with nutrient conservation and their delivery to the plants.

  12. Status of porous tube plant growth unit research - Development of a plant nutrient delivery system for space

    NASA Technical Reports Server (NTRS)

    Dreschel, T. W.; Wheeler, R. M.; Sager, J. C.

    1988-01-01

    A system developed for plant production in space was used to grow wheat, beans, rice, and white potatoes. It was found that the negative gauge pressure used to control the nutrient solution at the root/membrane interface and the pore size influence plant production. The results suggest that wheat, rice, beans, and lettuce can probably be grown with production values resembling those of plants grown in other media. Potato growth seemed to be stunted; this could be a possible response to root restriction.

  13. NUTRIENT ENRICHMENT AND FISHERIES EXPLOITATION: INTERACTIVE EFFECTS ON ESTUARINE LIVING RESOURCES AND THEIR MANAGEMENT

    EPA Science Inventory

    Fisheries exploitation and increased nutrient loadings affect fish and shellfish abudance and production in estuaries. These stressors do not act independently; instead they jointly influence food webs, and each affects the sensitivity of species and ecosystems to the other. Nu...

  14. NUTRIENT DYNAMICS IN RELATION TO GEOMORPHOLOGY OF RIVERINE WETLANDS

    EPA Science Inventory

    Variation in water depth and soil properties associated with geomorphic structures can affect riverine wetland nutrient dynamics by altering biogeochemical processes. We examined the seasonal influence of soils and geomorphology on nutrient forms and concentrations in riverine we...

  15. ER phospholipid composition modulates lipogenesis during feeding and in obesity.

    PubMed

    Rong, Xin; Wang, Bo; Palladino, Elisa Nd; de Aguiar Vallim, Thomas Q; Ford, David A; Tontonoz, Peter

    2017-10-02

    Sterol regulatory element-binding protein 1c (SREBP-1c) is a central regulator of lipogenesis whose activity is controlled by proteolytic cleavage. The metabolic factors that affect its processing are incompletely understood. Here, we show that dynamic changes in the acyl chain composition of ER phospholipids affect SREBP-1c maturation in physiology and disease. The abundance of polyunsaturated phosphatidylcholine in liver ER is selectively increased in response to feeding and in the setting of obesity-linked insulin resistance. Exogenous delivery of polyunsaturated phosphatidylcholine to ER accelerated SREBP-1c processing through a mechanism that required an intact SREBP cleavage-activating protein (SCAP) pathway. Furthermore, induction of the phospholipid-remodeling enzyme LPCAT3 in response to liver X receptor (LXR) activation promoted SREBP-1c processing by driving the incorporation of polyunsaturated fatty acids into ER. Conversely, LPCAT3 deficiency increased membrane saturation, reduced nuclear SREBP-1c abundance, and blunted the lipogenic response to feeding, LXR agonist treatment, or obesity-linked insulin resistance. Desaturation of the ER membrane may serve as an auxiliary signal of the fed state that promotes lipid synthesis in response to nutrient availability.

  16. Drug delivery to solid tumors: the predictive value of the multicellular tumor spheroid model for nanomedicine screening.

    PubMed

    Millard, Marie; Yakavets, Ilya; Zorin, Vladimir; Kulmukhamedova, Aigul; Marchal, Sophie; Bezdetnaya, Lina

    2017-01-01

    The increasing number of publications on the subject shows that nanomedicine is an attractive field for investigations aiming to considerably improve anticancer chemotherapy. Based on selective tumor targeting while sparing healthy tissue, carrier-mediated drug delivery has been expected to provide significant benefits to patients. However, despite reduced systemic toxicity, most nanodrugs approved for clinical use have been less effective than previously anticipated. The gap between experimental results and clinical outcomes demonstrates the necessity to perform comprehensive drug screening by using powerful preclinical models. In this context, in vitro three-dimensional models can provide key information on drug behavior inside the tumor tissue. The multicellular tumor spheroid (MCTS) model closely mimics a small avascular tumor with the presence of proliferative cells surrounding quiescent cells and a necrotic core. Oxygen, pH and nutrient gradients are similar to those of solid tumor. Furthermore, extracellular matrix (ECM) components and stromal cells can be embedded in the most sophisticated spheroid design. All these elements together with the physicochemical properties of nanoparticles (NPs) play a key role in drug transport, and therefore, the MCTS model is appropriate to assess the ability of NP to penetrate the tumor tissue. This review presents recent developments in MCTS models for a better comprehension of the interactions between NPs and tumor components that affect tumor drug delivery. MCTS is particularly suitable for the high-throughput screening of new nanodrugs.

  17. Significant drug-nutrient interactions.

    PubMed

    Kirk, J K

    1995-04-01

    Many nutrients substantially interfere with pharmacotherapeutic goals. The presence of certain nutrients in the gastrointestinal tract affects the bioavailability and disposition of many oral medications. Drug-nutrient interactions can also have positive effects that result in increased drug absorption or reduced gastrointestinal irritation. Knowing the significant drug-nutrient interactions can help the clinician identify the nutrients to avoid with certain medications, as well as the therapeutic agents that should be administered with food. This information can be used to educate patients and optimize pharmacotherapy.

  18. A Loblolly Pine Management Guide: Foresters' Primer in Nutrient Cycling

    Treesearch

    Jacques R. Jorgensen; Carol G. Wells

    1986-01-01

    The nutrient cycle, which includes the input of nutrients to the site, their losses, and their movement from one soil or vegetation component to another, can be modified by site preparation, rotation length, harvest system, fertilization, and fire, and by using soil-improving plants. Included is a report on how alternative procedures affect site nutrients, and provides...

  19. The effect of fire on soil properties

    Treesearch

    Leonard F. DeBano

    1991-01-01

    Fire affects nutrient cycling and the physical, chemical, and biological properties of soils occupied by western montane forests. Combustion of litter and soil organic matter (OM) increases the availability of some nutrients, although others are volatilized (for example, N, P, S). Soil OM loss also affects cation exchange capacity, organic chelation, aggregate...

  20. Providing lipid-based nutrient supplements does not affect developmental milestones among Malawian children

    USDA-ARS?s Scientific Manuscript database

    Our objective was to assess whether using lipid-based nutrient supplements (LNS) to complement the diets of infants and young children affected when they achieved selected developmental milestones. In rural Malawi, 840 6-month-old healthy infants were enrolled to a randomised trial. Control particip...

  1. Equatorial Pacific peak in biological production regulated by nutrient and upwelling during the late Pliocene/early Pleistocene cooling

    NASA Astrophysics Data System (ADS)

    Etourneau, J.; Robinson, R. S.; Martinez, P.; Schneider, R.

    2013-08-01

    The largest increase in export production in the eastern Pacific of the last 5.3 Myr (million years) occurred between 2.2 and 1.6 Myr, a time of major climatic and oceanographic reorganization in the region. Here, we investigate the causes of this event using reconstructions of export production, nutrient supply and oceanic conditions across the Pliocene-Pleistocene in the eastern equatorial Pacific (EEP) for the last 3.2 Myr. Our results indicate that the export production peak corresponds to a cold interval marked by high nutrient supply relative to consumption, as revealed by the low bulk sedimentary 15N/14N (δ15N) and alkenone-derived sea surface temperature (SST) values. This ∼0.6 million year long episode of enhanced delivery of nutrients to the surface of the EEP was predominantly initiated through the upwelling of nutrient-enriched water sourced in high latitudes. In addition, this phenomenon was likely promoted by the regional intensification of upwelling in response to the development of intense Walker and Hadley atmospheric circulations. Increased nutrient consumption in the polar oceans and enhanced denitrification in the equatorial regions restrained nutrient supply and availability and terminated the high export production event.

  2. Physiological Response of Plants Grown on Porous Ceramic Tubes

    NASA Technical Reports Server (NTRS)

    Tsao, David; Okos, Martin

    1997-01-01

    This research involves the manipulation of the root-zone water potential for the purposes of discriminating the rate limiting step in the inorganic nutrient uptake mechanism utilized by higher plants. This reaction sequence includes the pathways controlled by the root-zone conditions such as water tension and gradient concentrations. Furthermore, plant based control mechanisms dictated by various protein productions are differentiated as well. For the nutrients limited by the environmental availability, the kinetics were modeled using convection and diffusion equations. Alternatively, for the nutrients dependent upon enzyme manipulations, the uptakes are modeled using Michaelis-Menten kinetics. In order to differentiate between these various mechanistic steps, an experimental apparatus known as the Porous Ceramic Tube - Nutrient Delivery System (PCT-NDS) was used. Manipulation of the applied suction pressure circulating a nutrient solution through this system imposes a change in the matric component of the water potential. This compensates for the different osmotic components of water potential dictated by nutrient concentration. By maintaining this control over the root-zone conditions, the rate limiting steps in the uptake of the essential nutrients into tomato plants (Lycopersicon esculentum cv. Cherry Elite) were differentiated. Results showed that the uptake of some nutrients were mass transfer limited while others were limited by the enzyme kinetics. Each of these were adequately modeled with calculations and discussions of the parameter estimations provided.

  3. Nutrient sensing signaling integrates nutrient metabolism and intestinal immunity in grass carp, Ctenopharyngodon idellus after prolonged starvation.

    PubMed

    Li, Shan; Li, Jiabo; Zhao, Yongliang; Zhang, Qin; Wang, Qingchao

    2017-12-01

    Starvation has been shown to affect growth and nutrient metabolism in fish; however, little information about the nutrient sensing signaling and mucosal adaptive immunity in fish was known. In the present study, grass carp was starved for 8weeks to simulate the natural aquaculture practice in Hubei during winter. The histology of liver was significantly affected with decreased expression of tight junction proteins including claudin-3, claudin-b and ZO-1. Muscle gene expression was also affected, with decreased expression of muscle growth promoting factors such as Myogenin, MyoD, Myf5, and increased expression of muscle degradation factors, such as CathepsinD. In addition, mucosal adaptive immunity was also significantly affected, with decreased expression of antibodies including IgZ and IgM in gut. Along with these changes was the inhibition of several nutrient sensing signaling including MAPK and TOR signaling, which leads to the inhibition of the synthesis of protein including immunoglobulin. The increased phosphorylation of eIF2α not only inhibited the translation, but also resulted in the decreased expression of IkB and increased expression of NF-B, with the activation of pro-inflammatory genes including IL8 and TNF. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Targeted delivery of growth factors in ischemic stroke animal models.

    PubMed

    Rhim, Taiyoun; Lee, Minhyung

    2016-01-01

    Ischemic stroke is caused by reduced blood supply and leads to loss of brain function. The reduced oxygen and nutrient supply stimulates various physiological responses, including induction of growth factors. Growth factors prevent neuronal cell death, promote neovascularization, and induce cell growth. However, the concentration of growth factors is not sufficient to recover brain function after the ischemic damage, suggesting that delivery of growth factors into the ischemic brain may be a useful treatment for ischemic stroke. In this review, various approaches for the delivery of growth factors to ischemic brain tissue are discussed, including local and targeting delivery systems. To develop growth factor therapy for ischemic stroke, important considerations should be taken into account. First, growth factors may have possible side effects. Thus, concentration of growth factors should be restricted to the ischemic tissues by local administration or targeted delivery. Second, the duration of growth factor therapy should be optimized. Growth factor proteins may be degraded too fast to have a high enough therapeutic effect. Therefore, delivery systems for controlled release or gene delivery may be useful. Third, the delivery systems to the brain should be optimized according to the delivery route.

  5. Landscape influence on soil carbon and nutrient levels

    USDA-ARS?s Scientific Manuscript database

    Past runoff, erosion, and management practices influence nutrient levels on the landscape. These starting levels affect future nutrient transport due to runoff, erosion, and leaching events. The purpose of this study was to examine closed-depression landscape effects on surface soil organic matter, ...

  6. Nutrient load summaries for major lakes and estuaries of the Eastern United States, 2002

    USGS Publications Warehouse

    Moorman, Michelle C.; Hoos, Anne B.; Bricker, Suzanne B.; Moore, Richard B.; García, Ana María; Ator, Scott W.

    2014-01-01

    Nutrient enrichment of lakes and estuaries across the Nation is widespread. Nutrient enrichment can stimulate excessive plant and algal growth and cause a number of undesirable effects that impair aquatic life and recreational activities and can also result in economic effects. Understanding the amount of nutrients entering lakes and estuaries, the physical characteristics affecting the nutrient processing within these receiving waterbodies, and the natural and manmade sources of nutrients is fundamental to the development of effective nutrient reduction strategies. To improve this understanding, sources and stream transport of nutrients to 255 major lakes and 64 estuaries in the Eastern United States were estimated using Spatially Referenced Regression on Watershed attributes (SPARROW) nutrient models.

  7. Do mood and the receipt of work-based support influence nurse perceived quality of care delivery? A behavioural diary study.

    PubMed

    Jones, Martyn C; Johnston, Derek

    2013-03-01

    To examine the effect of nurse mood in the worst event of shift (negative affect, positive affect), receipt of work-based support from managers and colleagues, colleague and patient involvement on perceived quality of care delivery. While the effect of the work environment on nurse mood is well documented, little is known about the effects of the worst event of shift on the quality of care delivered by nurses. This behavioural diary study employed a within-subject and between-subject designs incorporating both cross-sectional and longitudinal elements. One hundred and seventy-one nurses in four large district general hospitals in England completed end-of-shift computerised behavioural diaries over three shifts to explore the effects of the worst clinical incident of shift. Diaries measured negative affect, positive affect, colleague involvement, receipt of work-based support and perceived quality of care delivery. Analysis used multilevel modelling (MLWIN 2.19; Centre for Multi-level Modelling, University of Bristol, Bristol, UK). High levels of negative affect and low levels of positive affect reported in the worst clinical incident of shift were associated with reduced perceived quality of care delivery. Receipt of managerial support and its interaction with negative affect had no relationship with perceived quality of care delivery. Perceived quality of care delivery deteriorated the most when the nurse reported a combination of high negative affect and no receipt of colleague support in the worst clinical incident of shift. Perceived quality of care delivery was also particularly influenced when the nurse reported low positive affect and colleague actions contributed to the problem. Receipt of colleague support is particularly salient in protecting perceived quality of care delivery, especially if the nurse also reports high levels of negative affect in the worst event of shift. The effect of work-based support on care delivery is complex and requires further investigation. © 2012 Blackwell Publishing Ltd.

  8. Effects of myocardial infarction on the distribution and transport of nutrients and oxygen in porcine myocardium.

    PubMed

    Davis, Bryce H; Morimoto, Yoshihisa; Sample, Chris; Olbrich, Kevin; Leddy, Holly A; Guilak, Farshid; Taylor, Doris A

    2012-10-01

    One of the primary limitations of cell therapy for myocardial infarction is the low survival of transplanted cells, with a loss of up to 80% of cells within 3 days of delivery. The aims of this study were to investigate the distribution of nutrients and oxygen in infarcted myocardium and to quantify how macromolecular transport properties might affect cell survival. Transmural myocardial infarction was created by controlled cryoablation in pigs. At 30 days post-infarction, oxygen and metabolite levels were measured in the peripheral skeletal muscle, normal myocardium, the infarct border zone, and the infarct interior. The diffusion coefficients of fluorescein or FITC-labeled dextran (0.3-70 kD) were measured in these tissues using fluorescence recovery after photobleaching. The vascular density was measured via endogenous alkaline phosphatase staining. To examine the influence of these infarct conditions on cells therapeutically used in vivo, skeletal myoblast survival and differentiation were studied in vitro under the oxygen and glucose concentrations measured in the infarct tissue. Glucose and oxygen concentrations, along with vascular density were significantly reduced in infarct when compared to the uninjured myocardium and infarct border zone, although the degree of decrease differed. The diffusivity of molecules smaller than 40 kD was significantly higher in infarct center and border zone as compared to uninjured heart. Skeletal myoblast differentiation and survival were decreased stepwise from control to hypoxia, starvation, and ischemia conditions. Although oxygen, glucose, and vascular density were significantly reduced in infarcted myocardium, the rate of macromolecular diffusion was significantly increased, suggesting that diffusive transport may not be inhibited in infarct tissue, and thus the supply of nutrients to transplanted cells may be possible. in vitro studies mimicking infarct conditions suggest that increasing nutrients available to transplanted cells may significantly increase their ability to survive in infarct.

  9. Factors Controlling Nitrogen Loadings in Major River Basins Across the United States

    NASA Astrophysics Data System (ADS)

    Boyer, E. W.; Alexander, R. B.; Galloway, J. N.; Golden, H. E.; Moore, R. B.; Schwarz, G. E.; Harvey, J. W.; Gomez-Velez, J. D.; Scott, D.; Clune, J.

    2017-12-01

    Inputs of reactive nitrogen (all N species except for N2) have been increasing worldwide, largely due to human activities associated with food production and energy consumption via the combustion of fossil fuels and biofuels. Despite the obvious essential benefits of a plentiful supply of food and energy, the adverse consequences associated with the accumulation of N in the environment are large. Most of the N created by human activities is released to the environment, often with unintended negative consequences. The greater the inputs of N to the landscape, the greater the potential for negative effects - caused by greenhouse gas production, ground level ozone, acid deposition, and N overload; which in turn can contribute to climate change, degradation of soils and vegetation, acidification of surface waters, coastal eutrophication, hypoxia, habitat loss, and loss of stratospheric ozone. Here we present a contemporary inventory of reactive N inputs to major water regions in the United States, and discuss accounting methods for quantifying N sources and transport. Furthermore, we quantify loadings of N from terrestrial headwaters downstream to coastal estuaries and embayments. N delivery to downstream waters is influenced by nutrient sources as well as coupled hydrological and biogeochemical processes occurring along the river corridor (e.g., travel time distributions, denitrification, and storage) that scale with stream size and are affected by impoundments such as lakes and reservoirs. This underscores the need to account for the nonlinear interactions of aquatic transport processes with watershed nutrient sources, as well as cumulative effects, in developing efficient nutrient reduction strategies. Our work is useful as a benchmark of the current N situation against which future progress can be assessed in varying water regions of the country; amidst changing N inputs, policies, and management strategies. Our results stem from the EPA Integrated Nitrogen Advisory Committee, the EPA Center for Integrated Multi-Scale Nutrient Pollution Solutions, and the John Wesley Powell Center River Corridor Working Group.

  10. Uncovering the Nutritional Landscape of Food

    PubMed Central

    Kim, Seunghyeon; Sung, Jaeyun; Foo, Mathias; Jin, Yong-Su; Kim, Pan-Jun

    2015-01-01

    Recent progresses in data-driven analysis methods, including network-based approaches, are revolutionizing many classical disciplines. These techniques can also be applied to food and nutrition, which must be studied to design healthy diets. Using nutritional information from over 1,000 raw foods, we systematically evaluated the nutrient composition of each food in regards to satisfying daily nutritional requirements. The nutrient balance of a food was quantified and termed nutritional fitness; this measure was based on the food’s frequency of occurrence in nutritionally adequate food combinations. Nutritional fitness offers a way to prioritize recommendable foods within a global network of foods, in which foods are connected based on the similarities of their nutrient compositions. We identified a number of key nutrients, such as choline and α-linolenic acid, whose levels in foods can critically affect the nutritional fitness of the foods. Analogously, pairs of nutrients can have the same effect. In fact, two nutrients can synergistically affect the nutritional fitness, although the individual nutrients alone may not have an impact. This result, involving the tendency among nutrients to exhibit correlations in their abundances across foods, implies a hidden layer of complexity when exploring for foods whose balance of nutrients within pairs holistically helps meet nutritional requirements. Interestingly, foods with high nutritional fitness successfully maintain this nutrient balance. This effect expands our scope to a diverse repertoire of nutrient-nutrient correlations, which are integrated under a common network framework that yields unexpected yet coherent associations between nutrients. Our nutrient-profiling approach combined with a network-based analysis provides a more unbiased, global view of the relationships between foods and nutrients, and can be extended towards nutritional policies, food marketing, and personalized nutrition. PMID:25768022

  11. Do breakfast skipping and breakfast type affect energy intake, nutrient intake, nutrient adequacy, and diet quality in young adults? NHANES 1999-2002

    USDA-ARS?s Scientific Manuscript database

    The objective of this study was to assess the impact of breakfast skipping and type of breakfast consumed on energy/nutrient intake, nutrient adequacy, and diet quality using a cross-sectional design. The setting was The National Health and Nutrition Examination Survey (NHANES), 1999-2002. The sub...

  12. 31 CFR 353.31 - Series HH bonds.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... the owner or coowner's account at a financial institution. Checks will be mailed to the delivery...) (c) Delivery of interest. Notices affecting the delivery of interest payments. To ensure appropriate action, notices affecting the delivery of interest payments on Series HH bonds must be received by the...

  13. Quantitative evaluation of boron neutron capture therapy (BNCT) drugs for boron delivery and retention at subcellular scale resolution in human glioblastoma cells with imaging secondary ion mass spectrometry (SIMS)

    PubMed Central

    Chandra, S.; Ahmad, T.; Barth, R. F.; Kabalka, G. W.

    2014-01-01

    Boron neutron capture therapy (BNCT) of cancer depends on the selective delivery of a sufficient number of boron-10 (10B) atoms to individual tumor cells. Cell killing results from the 10B (n, α)7Li neutron capture and fission reactions that occur if a sufficient number of 10B atoms are localized in the tumor cells. Intranuclear 10B localization enhances the efficiency of cell killing via damage to the DNA. The net cellular content of 10B atoms reflects both bound and free pools of boron in individual tumor cells. The assessment of these pools, delivered by a boron delivery agent, currently cannot be made at subcellular scale resolution by clinically applicable techniques such as PET and MRI. In this study, secondary ion mass spectrometry (SIMS) based imaging instrument, a CAMECA IMS 3f ion microscope, capable of 500 nm spatial resolution was employed. Cryogenically prepared cultured human T98G glioblastoma cells were evaluated for boron uptake and retention of two delivery agents. The first, L-p-boronophenylalanine (BPA), has been used clinically for BNCT of high grade gliomas, recurrent tumors of the head and neck region and melanomas. The second, a boron analogue of an unnatural amino acid, 1-amino-3-borono-cyclopentanecarboxylic acid (cis-ABCPC), has been studied in rodent glioma and melanoma models by quantification of boron in the nucleus and cytoplasm of individual tumor cells. The bound and free pools of boron were assessed by exposure of cells to boron-free nutrient medium. Both BPA and cis-ABCPC delivered almost 70% of the pool of boron in the free or loosely bound form to the nucleus and cytoplasm of human glioblastoma cells. This free pool of boron could be easily mobilized out of the cell and was in some sort of equilibrium with extracellular boron. In the case of BPA, the intracellular free pool of boron also was affected by the presence of phenylalanine in the nutrient medium. This suggests that it might be advantageous if patients were placed on a low phenylalanine diet prior to the initiation of BNCT. Since BPA currently is used clinically for BNCT, our observations may have direct relevance to future clinical studies utilizing this agent and provides support for individualized treatment planning regimens rather than the use of fixed BPA infusion protocols. PMID:24684609

  14. Quantitative evaluation of boron neutron capture therapy (BNCT) drugs for boron delivery and retention at subcellular-scale resolution in human glioblastoma cells with imaging secondary ion mass spectrometry (SIMS).

    PubMed

    Chandra, S; Ahmad, T; Barth, R F; Kabalka, G W

    2014-06-01

    Boron neutron capture therapy (BNCT) of cancer depends on the selective delivery of a sufficient number of boron-10 ((10)B) atoms to individual tumour cells. Cell killing results from the (10)B (n, α)(7) Li neutron capture and fission reactions that occur if a sufficient number of (10)B atoms are localized in the tumour cells. Intranuclear (10)B localization enhances the efficiency of cell killing via damage to the DNA. The net cellular content of (10)B atoms reflects both bound and free pools of boron in individual tumour cells. The assessment of these pools, delivered by a boron delivery agent, currently cannot be made at subcellular-scale resolution by clinically applicable techniques such as positron emission tomography and magnetic resonance imaging. In this study, a secondary ion mass spectrometry based imaging instrument, a CAMECA IMS 3f ion microscope, capable of 500 nm spatial resolution was employed. Cryogenically prepared cultured human T98G glioblastoma cells were evaluated for boron uptake and retention of two delivery agents. The first, L-p-boronophenylalanine (BPA), has been used clinically for BNCT of high-grade gliomas, recurrent tumours of the head and neck region and melanomas. The second, a boron analogue of an unnatural amino acid, 1-amino-3-borono-cyclopentanecarboxylic acid (cis-ABCPC), has been studied in rodent glioma and melanoma models by quantification of boron in the nucleus and cytoplasm of individual tumour cells. The bound and free pools of boron were assessed by exposure of cells to boron-free nutrient medium. Both BPA and cis-ABCPC delivered almost 70% of the pool of boron in the free or loosely bound form to the nucleus and cytoplasm of human glioblastoma cells. This free pool of boron could be easily mobilized out of the cell and was in some sort of equilibrium with extracellular boron. In the case of BPA, the intracellular free pool of boron also was affected by the presence of phenylalanine in the nutrient medium. This suggests that it might be advantageous if patients were placed on a low phenylalanine diet prior to the initiation of BNCT. Since BPA currently is used clinically for BNCT, our observations may have direct relevance to future clinical studies utilizing this agent and provides support for individualized treatment planning regimens rather than the use of fixed BPA infusion protocols. © 2014 The Authors Journal of Microscopy © 2014 Royal Microscopical Society.

  15. Soil redistribution and nutrient delivery in a Mediterranean rain-fed agro-ecosystem with different crops and management: environmental and economic aspects

    NASA Astrophysics Data System (ADS)

    López-Vicente, Manuel; Álvarez, Sara

    2017-04-01

    Mediterranean agro-ecosystems are characterised by fragmented fields and patched vegetation. This shape governs the spatial patterns of water, soil and nutrient redistribution. Rainfall parameters, human infrastructures, crop management, support practices, and land use changes (set aside crops, land abandonment) control the magnitude of these processes. Under rain-fed water supply conditions, runoff generation and soil water content are two important factors in determining crop yield. Soil erosion and nutrient delivery are two of the factors which limit crop yield and thus, the gross earning of the landowner. In hilly landscapes, farmers usually supply extra soil to fill in the ephemeral gullies, and nutrient replenishment with fertilizers is a common practice. The aim of this study is to evaluate the environmental (runoff yield, soil erosion and nutrient delivery) and economic (replenishment of soil and nutrient losses with new soil and fertilizers) consequences of different conventional and conservative practices (fallow/crop rotation, cover crops, land abandonment, buffer strips) in a Mediterranean rain-fed agro-ecosystem (27 ha) with vineyards, cereal crops, cultivated and abandoned olive orchards, several trails and patches of natural vegetation. The five winter cereal fields (wheat and barley) follow fallow/crop rotation. The four vineyards are devoted to the Garnacha variety: one planted in 2007 with white wine grapes, and three planted in 2008 with red wine grapes. The inter-crop strips are managed with a mixture of plant species as cover crop (CC), including: i) spontaneous vegetation, and ii) plantation of common sainfoin (Onobrychis viciifolia). The maintenance of the CC includes one mowing pass at the end of spring, between May and June. The appearance and development of ephemeral gullies and the deposition of soil at the bottom of the hillslope are two of the main concerns of the landowners. In some places, the accumulation of soil complicates grape harvest operations with machinery, forcing manual labour. A total of 222 soil samples were collected in 74 points, and some physical (coarse fragments, effective volume, bulk density, texture, infiltration, etc.) and chemical (soil organic carbon - SOC, total nitrogen, phosphorous and potassium) parameters analysed. The highest values of SOC and TN were found in the forestry (4.64% and 0.198%) and abandoned soils (2.96% and 0.132%), whereas the highest values of TP appeared in the cereal, olive and vineyards (458.4, 458.0 and 440.3 mg / kg P). The highest content of TK appeared in the vineyards (1979.1 mg / kg K), especially in the grapevine strips (2188.3 mg / kg K), due to the fertilizer supply. In order to assess the water, soil and nutrient budgets, four buried sediment traps were installed near the bottom and before reaching the depositional-prone area. The upslope contributing areas of the traps are not nested. Monitoring the magnitude of runoff and sediment yield and the chemical composition of the collected samples allowed calculating the economic cost of water, soil and nutrient losses. The results of this study have implications for other rain-fed productive agro-ecosystems as well as where conservative practices may reduce the economic cost of farmland management.

  16. Arctic foxes as ecosystem engineers: increased soil nutrients lead to increased plant productivity on fox dens

    NASA Astrophysics Data System (ADS)

    Gharajehdaghipour, Tazarve; Roth, James D.; Fafard, Paul M.; Markham, John H.

    2016-04-01

    Top predators can provide fundamental ecosystem services such as nutrient cycling, and their impact can be even greater in environments with low nutrients and productivity, such as Arctic tundra. We estimated the effects of Arctic fox (Vulpes lagopus) denning on soil nutrient dynamics and vegetation production near Churchill, Manitoba in June and August 2014. Soils from fox dens contained higher nutrient levels in June (71% more inorganic nitrogen, 1195% more extractable phosphorous) and in August (242% more inorganic nitrogen, 191% more extractable phosphorous) than adjacent control sites. Inorganic nitrogen levels decreased from June to August on both dens and controls, whereas extractable phosphorous increased. Pup production the previous year, which should enhance nutrient deposition (from urine, feces, and decomposing prey), did not affect soil nutrient concentrations, suggesting the impact of Arctic foxes persists >1 year. Dens supported 2.8 times greater vegetation biomass in August, but δ15N values in sea lyme grass (Leymus mollis) were unaffected by denning. By concentrating nutrients on dens Arctic foxes enhance nutrient cycling as an ecosystem service and thus engineer Arctic ecosystems on local scales. The enhanced productivity in patches on the landscape could subsequently affect plant diversity and the dispersion of herbivores on the tundra.

  17. Arctic foxes as ecosystem engineers: increased soil nutrients lead to increased plant productivity on fox dens.

    PubMed

    Gharajehdaghipour, Tazarve; Roth, James D; Fafard, Paul M; Markham, John H

    2016-04-05

    Top predators can provide fundamental ecosystem services such as nutrient cycling, and their impact can be even greater in environments with low nutrients and productivity, such as Arctic tundra. We estimated the effects of Arctic fox (Vulpes lagopus) denning on soil nutrient dynamics and vegetation production near Churchill, Manitoba in June and August 2014. Soils from fox dens contained higher nutrient levels in June (71% more inorganic nitrogen, 1195% more extractable phosphorous) and in August (242% more inorganic nitrogen, 191% more extractable phosphorous) than adjacent control sites. Inorganic nitrogen levels decreased from June to August on both dens and controls, whereas extractable phosphorous increased. Pup production the previous year, which should enhance nutrient deposition (from urine, feces, and decomposing prey), did not affect soil nutrient concentrations, suggesting the impact of Arctic foxes persists >1 year. Dens supported 2.8 times greater vegetation biomass in August, but δ(15)N values in sea lyme grass (Leymus mollis) were unaffected by denning. By concentrating nutrients on dens Arctic foxes enhance nutrient cycling as an ecosystem service and thus engineer Arctic ecosystems on local scales. The enhanced productivity in patches on the landscape could subsequently affect plant diversity and the dispersion of herbivores on the tundra.

  18. Arctic foxes as ecosystem engineers: increased soil nutrients lead to increased plant productivity on fox dens

    PubMed Central

    Gharajehdaghipour, Tazarve; Roth, James D.; Fafard, Paul M.; Markham, John H.

    2016-01-01

    Top predators can provide fundamental ecosystem services such as nutrient cycling, and their impact can be even greater in environments with low nutrients and productivity, such as Arctic tundra. We estimated the effects of Arctic fox (Vulpes lagopus) denning on soil nutrient dynamics and vegetation production near Churchill, Manitoba in June and August 2014. Soils from fox dens contained higher nutrient levels in June (71% more inorganic nitrogen, 1195% more extractable phosphorous) and in August (242% more inorganic nitrogen, 191% more extractable phosphorous) than adjacent control sites. Inorganic nitrogen levels decreased from June to August on both dens and controls, whereas extractable phosphorous increased. Pup production the previous year, which should enhance nutrient deposition (from urine, feces, and decomposing prey), did not affect soil nutrient concentrations, suggesting the impact of Arctic foxes persists >1 year. Dens supported 2.8 times greater vegetation biomass in August, but δ15N values in sea lyme grass (Leymus mollis) were unaffected by denning. By concentrating nutrients on dens Arctic foxes enhance nutrient cycling as an ecosystem service and thus engineer Arctic ecosystems on local scales. The enhanced productivity in patches on the landscape could subsequently affect plant diversity and the dispersion of herbivores on the tundra. PMID:27045973

  19. Parasite and nutrient enrichment effects on Daphnia interspecific competition.

    PubMed

    Decaestecker, Ellen; Verreydt, Dino; De Meester, Luc; Declerck, Steven A J

    2015-05-01

    Increased productivity due to nutrient enrichment is hypothesized to affect density-dependent processes, such as transmission success of horizontally transmitting parasites. Changes in nutrient availability can also modify the stoichiometry and condition of individual hosts, which may affect their susceptibility for parasites as well as the growth conditions for parasites within the host. Consequently, if not balanced by increased host immuno-competence or life history responses, changes in the magnitude of parasite effects with increasing nutrient availability are expected. If these parasite effects are host-species specific, this may lead to shifts in the host community structure. We here used the Daphnia- parasite model system to study the effect of nutrient enrichment on parasite-mediated competition in experimental mesocosms. In the absence of parasites, D. magna was competitively dominant to D. pulex at both low and high nutrient levels. Introduction of parasites resulted in infections of D. magna, but not of D. pulex and, as such, reversed the competitive hierarchy between these two species. Nutrient addition resulted in an increased prevalence and infection intensity of some of the parasites on D. magna. However, there was no evidence that high nutrient levels enhanced negative effects of parasites on the hosts. Costs associated with parasite infections may have been compensated by better growth conditions for D. magna in the presence of high nutrient levels.

  20. A nutrient injection scheme for in situ bio-remediation.

    PubMed

    Lin, C H; Kuo, M C Tom; Su, C Y; Liang, K F; Han, Y L

    2012-01-01

    Geological layers often have different hydraulic conductivities. This paper presents an innovative design for delivering aqueous substrates and nutrients to various stratified layers at desired rates during in-situ bio-stimulation. The new delivery system consists of intermittent porous tubes connected in series with impermeable polyethylene tubes that run horizontally in each stratified layer of a contaminated aquifer. Results of the tracer test indicated that the distribution of tritium through each porous tube was fairly uniform. A mathematical model was also developed to calculate the distribution of water flow through each porous tube. By controlling the permeability and the length of porous tubes placed in stratified layers, the new design provides a means to selectively deliver nutrients to various layers at desired rates according to aquifer heterogeneity.

  1. Dynamics of microorganism populations in recirculating nutrient solutions

    NASA Technical Reports Server (NTRS)

    Strayer, R. F.

    1994-01-01

    This overview covers the basic microbial ecology of recirculating hydroponic solutions. Examples from NASA and Soviet CELSS tests and the commercial hydroponic industry will be used. The sources of microorganisms in nutrient solutions include air, water, seeds, plant containers and plumbing, biological vectors, and personnel. Microbial fates include growth, death, and emigration. Important microbial habitats within nutrient delivery systems are root surfaces, hardware surfaces (biofilms), and solution suspension. Numbers of bacteria on root surfaces usually exceed those from the other habitats by several orders of magnitude. Gram negative bacteria dominate the microflora with fungal counts usually much lower. Trends typically show a decrease in counts with increasing time unless stressed plants increase root exudates. Important microbial activities include carbon mineralization and nitrogen transformations. Important detrimental interactions include competition with plants, and human and plant pathogenesis.

  2. Encapsulation of new active ingredients.

    PubMed

    Onwulata, C I

    2012-01-01

    The organic construct consumed as food comes packaged in units that carry the active components and protect the entrapped active materials until delivered to targeted human organs. The packaging and delivery role is mimicked in the microencapsulation tools used to deliver active ingredients in processed foods. Microencapsulation efficiency is balanced against the need to access the entrapped nutrients in bioavailable forms. Encapsulated ingredients boosted with bioactive nutrients are intended for improved health and well-being and to prevent future health problems. Presently, active ingredients are delivered using new techniques, such as hydrogels, nanoemulsions, and nanoparticles. In the future, nutraceuticals and functional foods may be tailored to individual metabolic needs and tied to each person's genetic makeup. Bioactive ingredients provide health-enhancing nutrients and are protected through encapsulation processes that shield the active ingredients from deleterious environments.

  3. Soil pH, soil type and replant disease affect growth and nutrient absorption in apple rootstocks

    USDA-ARS?s Scientific Manuscript database

    Rootstocks are the foundation of a healthy and productive orchard. They are the interface between the scion and the soil, providing anchorage, water, nutrients, and disease protection that ultimately affect the productivity and sustainability of the orchard. Recent advances in the science of genet...

  4. Nutrition Source Book.

    ERIC Educational Resources Information Center

    National Dairy Council, Rosemont, IL.

    This booklet presents a nutrient approach to teaching nutrition. It contains basic nutrition information along with suggestions for translating this information to fulfill the needs of families and individuals. Topics discussed are: (1) a nutrient approach to teaching nutrition; (2) functions of nutrients; (3) how food handling affects nutrient…

  5. Management practices affect soil nutrients and bacterial populations in backgrounding beef feedlot

    USDA-ARS?s Scientific Manuscript database

    Contaminants associated with manure in animal production sites are of significant concern. Unless properly managed, high soil nutrient concentrations in feedlots can deteriorate soil and water quality. This three year study tested a nutrient management strategy with three sequentially imposed manage...

  6. Responses of nutrient capture and fine root morphology of subalpine coniferous tree Picea asperata to nutrient heterogeneity and competition

    PubMed Central

    Nan, Hongwei; Liang, Jin; Cheng, Xinying; Zhao, ChunZhang; Yin, HuaJun; Yin, ChunYing; Liu, Qing

    2017-01-01

    Investigating the responses of trees to the heterogeneous distribution of nutrients in soil and simultaneous presence of neighboring roots could strengthen the understanding of an influential mechanism on tree growth and provide a scientific basis for forest management. Here, we conducted two split-pot experiments to investigate the effects of nutrient heterogeneity and intraspecific competition on the fine root morphology and nutrient capture of Picea asperata. The results showed that P. asperata efficiently captured nutrients by increasing the specific root length (SRL) and specific root area (SRA) of first-and second-order roots and decreasing the tissue density of first-order roots to avoid competition for resources and space with neighboring roots. The nutrient heterogeneity and addition of fertilization did not affect the fine root morphology, but enhanced the P and K concentrations in the fine roots in the absence of a competitor. On the interaction between nutrient heterogeneity and competition, competition decreased the SRL and SRA but enhanced the capture of K under heterogeneous soil compared with under homogeneous soil. Additionally, the P concentration, but not the K concentration, was linearly correlated to root morphology in heterogeneous soil, even when competition was present. The results suggested that root morphological features were only stimulated when the soil nutrients were insufficient for plant growth and the nutrients accumulations by root were mainly affected by the soil nutrients more than the root morphology. PMID:29095947

  7. EFFECT OF NITROGEN SOURCE ON THE GROWTH AND TOXICITY OF THREE POTENTIALLY HARMFUL DINOFLAGELLATES

    EPA Science Inventory

    Increases in population and agriculture in coastal areas can result in increased nutrient inputs and alterations in the ratios of organic to inorganic nutrients in coastal waters. Such changes in coastal nutrient regimes can affect phytoplankton community structure by creating c...

  8. Catchment-scale evaluation of environmental regulations in the agricultural sector in Ireland (Invited)

    NASA Astrophysics Data System (ADS)

    Melland, A. R.; Jordan, P.; Mellander, P.; Wall, D. J.; Buckley, C.; Mechan, S.; Shortle, G.

    2010-12-01

    The European Union (EU) Nitrates Directive regulations in Ireland limits the use of agricultural fertilisers to agronomic optima and aims to minimise surplus phosphorus (P) and nitrogen (N) losses to the aquatic environment. The legislated measures include limits on nutrient application according to soil P status, crop type and livestock intensity and restricts chemical and organic fertiliser spreading and ploughing to periods of the year with typically lower exposure of nutrients to runoff and leaching. These agricultural policies are being evaluated in an Agricultural Catchments Programme in six representative catchments dominated by moderate to high intensity grassland and arable enterprises across Ireland (Fealy et al., 2010). An experimental programme has been established to provide a baseline of farm nutrient management and water body quality during the early years of the measures and to provide estimates of trajectories towards (or otherwise) water quality targets. A ‘nutrient transfer continuum’ from source, through pathways, to delivery and impact in a water body receptor describes the different phases of diffuse pollution and is being used as a framework for evaluation. Compliance with Irish standards at different levels of the continuum is being evaluated and demonstrative studies are being conducted to provide evidence of linkages between source and delivery to validate conceptual models of P and N transfers in time and space in each catchment. Source compliance is being evaluated through census soil testing and a survey of nutrient management practice and farmyard infrastructure. Mobilisation and pathways of nutrient transfers do not have chemical standards except where a groundwater body acts as both a receptor and a pathway. To demonstrate these linkages, however, representative groundwater pathways are being monitored through piezometer, chemical end-member and tracer studies, and surface water pathways are being evaluated through subcatchment storm sampling and terrain analysis modelling. Delivery and impact compliance are being assessed against EU and Irish chemical and biological standards for water body receptors. Trajectories of change will be considered. For example the time for current policies to have an impact on biological water quality may be dependant on soil P status decline rates, mobilisation rates for P stores in waterways and rates of ecological response to change in the trophic status of water body receptors. The attitudes of farmer stakeholders towards the measures and the economic impacts of investment in infrastructure and changed management are also being evaluated. Some preliminary data are presented including scenarios that suggest a lack of connectivity between farm source and water quality compliance standards. Fealy, R.M., Buckley, C., Mechan, S., Melland, A., Mellander, P.-E., Shortle, G., Wall, D. and Jordan, P. 2010. The Irish Agricultural Catchments Programme: catchment selection using spatial multi-criteria decision analysis. Soil Use and Management.23:225-236

  9. Examination of facilitators and barriers to home-based supplemental feeding with ready-to-use food for underweight children in western Uganda

    PubMed Central

    Jilcott, Stephanie B.; Myhre, Jennifer A.; Adair, Linda S.; Thirumurthy, Harsha; Bentley, Margaret E.; Ammerman, Alice S.

    2013-01-01

    Poor complementary feeding practices and low quality complementary foods are significant causes of growth faltering and child mortality throughout the developing world. Ready-to-use foods (RUF) are energy dense, lipid-based products that do not require cooking or refrigeration, that have been used to prevent and treat malnutrition among vulnerable children. The effectiveness of these products in improving child nutritional status depends on household use by caregivers. To identify the key facilitators and barriers that influence appropriate in-home RUF consumption by supplemental feeding program beneficiaries, we conducted individual interviews among caregivers (n=80), RUF producers (n=8) and program staff (n=10) involved in the Byokulia Bisemeye mu Bantu (BBB) supplemental feeding program in Bundibugyo, Uganda. By documenting caregiver perceptions and feeding practices related to RUF, we developed a conceptual framework of factors that affect appropriate feeding with RUF. Findings suggest that locally produced RUF is well received by caregivers and children, and is perceived by caregivers and the community to be a healthy supplemental food for malnourished children. However, child feeding practices, including sharing of RUF within households, compromise the nutrient delivery to the intended child. Interventions and educational messages informed by this study can help to improve RUF delivery to targeted beneficiaries. PMID:22136223

  10. Cool tadpoles from Arctic environments waste fewer nutrients - high gross growth efficiencies lead to low consumer-mediated nutrient recycling in the North.

    PubMed

    Liess, Antonia; Guo, Junwen; Lind, Martin I; Rowe, Owen

    2015-11-01

    Endothermic organisms can adapt to short growing seasons, low temperatures and nutrient limitation by developing high growth rates and high gross growth efficiencies (GGEs). Animals with high GGEs are better at assimilating limiting nutrients and thus should recycle (or lose) fewer nutrients. Longer guts in relation to body mass may facilitate higher GGE under resource limitation. Within the context of ecological stoichiometry theory, this study combines ecology with evolution by relating latitudinal life-history adaptations in GGE, mediated by gut length, to its ecosystem consequences, such as consumer-mediated nutrient recycling. In common garden experiments, we raised Rana temporaria tadpoles from two regions (Arctic/Boreal) under two temperature regimes (18/23 °C) crossed with two food quality treatments (high/low-nitrogen content). We measured tadpole GGEs, total nutrient loss (excretion + egestion) rates and gut length during ontogeny. In order to maintain their elemental balance, tadpoles fed low-nitrogen (N) food had lower N excretion rates and higher total phosphorous (P) loss rates than tadpoles fed high-quality food. In accordance with expectations, Arctic tadpoles had higher GGEs and lower N loss rates than their low-latitude conspecifics, especially when fed low-N food, but only in ambient temperature treatments. Arctic tadpoles also had relatively longer guts than Boreal tadpoles during early development. That temperature and food quality interacted with tadpole region of origin in affecting tadpole GGEs, nutrient loss rates and relative gut length, suggests evolved adaptation to temperature and resource differences. With future climate change, mean annual temperatures will increase. Additionally, species and genotypes will migrate north. This will change the functioning of Boreal and Arctic ecosystems by affecting consumer-mediated nutrient recycling and thus affect nutrient dynamics in general. Our study shows that evolved latitudinal adaption can change key ecosystem functions. © 2015 The Authors. Journal of Animal Ecology © 2015 British Ecological Society.

  11. Plant nutrition: root transporters on the move.

    PubMed

    Zelazny, Enric; Vert, Grégory

    2014-10-01

    Nutrient and water uptake from the soil is essential for plant growth and development. In the root, absorption and radial transport of nutrients and water toward the vascular tissues is achieved by a battery of specialized transporters and channels. Modulating the amount and the localization of these membrane transport proteins appears as a way to drive their activity and is essential to maintain nutrient homeostasis in plants. This control first involves the delivery of newly synthesized proteins to the plasma membrane by establishing check points along the secretory pathway, especially during the export from the endoplasmic reticulum. Plasma membrane-localized transport proteins are internalized through endocytosis followed by recycling to the cell surface or targeting to the vacuole for degradation, hence constituting another layer of control. These intricate mechanisms are often regulated by nutrient availability, stresses, and endogenous cues, allowing plants to rapidly adjust to their environment and adapt their development. © 2014 American Society of Plant Biologists. All Rights Reserved.

  12. Reduction of soluble nitrogen and mobilization of plant nutrients in soils from U.S. northern Great Plains agroecosystems by phenolic compounds

    USDA-ARS?s Scientific Manuscript database

    Phenolic plant secondary metabolites actively participate in a broad range of important reactions that affect livestock, plants and soil. In soil, phenolic compounds can affect nutrient dynamics and mobility of metals but their role in northern Great Plains agroecosystems is largely unknown. We eval...

  13. Growth, morphometrics and nutrient content of farmed eastern oysters, Crassostrea virginica (Gmelin), in New Hampshire, USA

    EPA Science Inventory

    When harvested, oysters represent a removal from the ecosystem of nutrients such as nitrogen (N)and carbon (C). A number of factors potentially affect nutrient content, but a quantitative understanding across the geographical range of the eastern oysters is lacking. This study wa...

  14. Nutrient transport in runoff as affected by diet, tillage and manure application rate

    USDA-ARS?s Scientific Manuscript database

    Including distillers grains in feedlot finishing diets may increase feedlot profitability. However the nutrient content of by-products are concentrated about three during the distillation process. Manure can be applied to meet single or multiple year crop nutrient requirements. The water quality eff...

  15. Biomass and nutrient mass of Acacia dealbata and Eucalyptus globulus bioenergy plantations

    Treesearch

    Timothy J. Albaugh; Rafael A. Rubilar; Chris A. Maier; Eduardo A. Acuña; Rachel L. Cook

    2017-01-01

    We quantified biomass and nutrient accumulation of Acacia dealbata Link and Eucalyptus globulus Labill. planted at stem densities of 5000 and 15000 ha-1 in a bioenergy plantation in Chile. We tested the hypotheses that species and stocking will not affect biomass or nutrient accumulation. Species and...

  16. [Relationships between soil nutrients and rhizospheric soil microbial communities and enzyme activities in a maize-capsicum intercropping system].

    PubMed

    Xu, Qiang; Cheng, Zhi-Hui; Meng, Huan-Wen; Zhang, Yu

    2007-12-01

    By using plastic sheet and nylon mesh to partition the root systems of maize and capsicum in a maize-capsicum intercropping system, this paper studied the relationships between soil biological factors and nutritive status in the intercropping system, with no partitioning and maize monoculture and capsicum monoculture as the control. The results showed that intercropping maize and capsicum had its high superiority. In the treatments of no partitioning and nylon mesh portioning in the intercropping system, soil enzyme activities, microbial individuals and nutrient contents were significantly higher, compared with those in the treatments of nylon mesh partitioning and monocultures. All kinds of soil available nutrients showed significant or very significant positive correlations with soil biological factors, except that soil available Mg was negatively correlated with soil fungi and catalase activity. Pathway analysis indicated that in the intercropping system, soil urease, catalase, protease, and bacteria were the main factors affecting the accumulation of soil organic matter, saccharase was the most important factor affecting soil alkali-hydrolyzable N, urease was the most important factor affecting soil available P, and bacteria largely determined soil available K. Soil alkaline phosphatase and fungi selectively affected the accumulation of soil organic matter and available N, P and K. There was a slight negative correlation between soil actinomycetes and soil nutrients, suggesting that actinomycetes had little effect on soil nutrient formation.

  17. Bioavailability of Dissolved Organic Carbon and Nitrogen From Tropical Montane Rainforest Streams Across a Geologic age Gradient

    NASA Astrophysics Data System (ADS)

    Wiegner, T. N.

    2005-05-01

    Dissolved organic matter (DOM) is metabolically important in streams. Its bioavailability is influenced by organic matter sources to streams and inorganic nutrient availability. As forest canopies and soils develop over time, organic matter inputs to streams should switch from algal to watershed sources. Across this succession gradient, nutrient limitation should also change. This study examines how chemical composition and bioavailability of DOM from tropical montane rainforest streams on Hawaii change across a geologic age gradient from 4 ky to 150 ky. Dissolved organic C (DOC) and N (DON) concentrations, chemical characteristics, and bioavailability varied with site age. With increasing stream age, DOC and DON concentrations, DOM aromaticity, and the C:N of the stream DOM increased. Changes in stream DOM chemistry and inorganic nutrient availability affected DOM bioavailability. Fifty percent of the DOC from the 4 ky site was bioavailable, where little to none was bioavailable from the older streams. Inorganic nutrient availability did not affect DOC bioavailability. In contrast, DON bioavailability was similar (12%) across sites and was affected by inorganic nutrient availability. This study demonstrates that the chemistry and metabolism of streams draining forests change with ecosystem age and development.

  18. Nitrogen and phosphorus in the Upper Mississippi River: Transport, processing, and effects on the river ecosystem

    USGS Publications Warehouse

    Houser, J.N.; Richardson, W.B.

    2010-01-01

    Existing research on nutrients (nitrogen and phosphorus) in the Upper Mississippi River (UMR) can be organized into the following categories: (1) Long-term changes in nutrient concentrations and export, and their causes; (2) Nutrient cycling within the river; (3) Spatial and temporal patterns of river nutrient concentrations; (4) Effects of elevated nutrient concentrations on the river; and (5) Actions to reduce river nutrient concentrations and flux. Nutrient concentration and flux in the Mississippi River have increased substantially over the last century because of changes in land use, climate, hydrology, and river management and engineering. As in other large floodplain rivers, rates of processes that cycle nitrogen and phosphorus in the UMR exhibit pronounced spatial and temporal heterogeneity because of the complex morphology of the river. This spatial variability in nutrient processing creates clear spatial patterns in nutrient concentrations. For example, nitrate concentrations generally are much lower in off-channel areas than in the main channel. The specifics of in-river nutrient cycling and the effects of high rates of nutrient input on UMR have been less studied than the factors affecting nutrient input to the river and transport to the Gulf of Mexico, and important questions concerning nutrient cycling in the UMR remain. Eutrophication and resulting changes in river productivity have only recently been investigated the UMR. These recent studies indicate that the high nutrient concentrations in the river may affect community composition of aquatic vegetation (e. g., the abundance of filamentous algae and duckweeds), dissolved oxygen concentrations in off-channel areas, and the abundance of cyanobacteria. Actions to reduce nutrient input to the river include changes in land-use practices, wetland restoration, and hydrological modifications to the river. Evidence suggests that most of the above methods can contribute to reducing nutrient concentration in, and transport by, the UMR, but the impacts of mitigation efforts will likely be only slowly realized. ?? USGS, US Government 2010.

  19. Marine nutrient contributions to tidal creeks in Virginia: spawning marine fish as nutrient vectors to freshwater ecosystems

    NASA Astrophysics Data System (ADS)

    Macavoy, S. E.; Garman, G. C.

    2006-12-01

    Coastal freshwater streams are typically viewed as conduits for the transport of sediment and nutrients to the coasts. Some coastal streams however experience seasonal migrations of anadromous fish returning to the freshwater to spawn. The fish may be vectors for the delivery of marine nutrients to nutrient poor freshwater in the form of excreted waste and post-spawning carcasses. Nutrients derived from marine sources are 13C, 15N and 34S enriched relative to nutrients in freshwater. Here we examine sediment, particulate organic matter (POM), invertebrates and fish in two tidal freshwater tributaries of the James River USA. The d15N of POM became elevated (from 3.8 to 6.5%), coincident with the arrival of anadromous river herring (Alosa sp), indicating a pulse of marine nitrogen. However, the elevated 15N was not observed in sediment samples or among invertebrates, which did not experience a seasonal isotopic shift (there were significant differences however among the guilds of invertebrate). Anadromous Alosa aestivalis captured within the tidal freshwater were 13C and 34S enriched (-19.3 and 17.2%, respectively) relative to resident freshwater fishes (-26.4 and 3.6% respectively) captured within 2 weeks of the Alosa. Although it is likely that marine derived nitrogen was detected in the tidal freshwater, it was not in sufficient abundance to change the isotope signature of most ecosystem components.

  20. KSC-04pd1397

    NASA Image and Video Library

    2004-06-30

    KENNEDY SPACE CENTER, FLA. - Kimberly Beck, a college trainee in Controlled Biological Systems in the Spaceflight and Life Sciences Training Program, is helping with growth studies supporting payload development. Behind her is part of the WONDER (Water Offset Nutrient Delivery Experiment) flight payload that is investigating hydroponic plant crop production in microgravity.

  1. Using National Coastal Assessment Data to Model Estuarine Water Quality at Large Spatial Scales.

    EPA Science Inventory

    The water quality of the Nation’s estuaries is attracting scrutiny in light of population growth and enhanced nutrient delivery. The USEPA has evaluated water quality in the National Coastal Assessment (NCA) and National Aquatic Resource Surveys (NARS) programs. Here we rep...

  2. Habitat coupling in a large lake system: delivery of a nearshore energy subsidy by an offshore planktivore

    EPA Science Inventory

    In ecosystems where native fish species have been greatly reduced or extirpated, ecological processes such as transport of energy and nutrients across habitats or ecosystems may be lost to the detriment of remaining native species. We hypothesized that fall spawning migrations ...

  3. KSC-04PD-1397

    NASA Technical Reports Server (NTRS)

    2004-01-01

    KENNEDY SPACE CENTER, FLA. Kimberly Beck, a college trainee in Controlled Biological Systems in the Spaceflight and Life Sciences Training Program, is helping with growth studies supporting payload development. Behind her is part of the WONDER (Water Offset Nutrient Delivery Experiment) flight payload that is investigating hydroponic plant crop production in microgravity.

  4. Using National Coastal Assessment Data to Model Estuarine Water Quality at Large Spatial Scales

    EPA Science Inventory

    Background/Question/MethodThe water quality of the Nation’s estuaries is attracting increasing scrutiny in light of burgeoning coastal population growth and enhanced delivery of nutrients via riverine flux. The USEPA has evaluated water quality in US estuaries in the Nation...

  5. Wastewater influences nitrogen dynamics in a coastal catchment during a prolonged drought

    PubMed Central

    Hoellein, Timothy J.; Mooney, Rae F.; Gardner, Wayne S.; Buskey, Edward J.

    2017-01-01

    Abstract Ecosystem function measurements can enhance our understanding of nitrogen (N) delivery in coastal catchments across river and estuary ecosystems. Here, we contrast patterns of N cycling and export in two rivers, one heavily influenced by wastewater treatment plants (WWTP), in a coastal catchment of south Texas. We measured N export from both rivers to the estuary over 2 yr that encompass a severe drought, along with detailed mechanisms of N cycling in river, tidal river, and two estuary sites during prolonged drought. WWTP nutrient inputs stimulated uptake of N, but denitrification resulting in permanent N removal accounted for only a small proportion of total uptake. During drought periods, WWTP N was the primary source of exported N to the estuary, minimizing the influence of episodic storm‐derived nutrients from the WWTP‐influenced river to the estuary. In the site without WWTP influence, the river exported very little N during drought, so storm‐derived nutrient pulses were important for delivering N loads to the estuary. Overall, N is processed from river to estuary, but sustained WWTP‐N loads and periodic floods alter the timing of N delivery and N processing. Research that incorporates empirical measurements of N fluxes from river to estuary can inform management needs in the face of multiple anthropogenic stressors such as demand for freshwater and eutrophication. PMID:29263559

  6. Contribution of Golden Apple Snail Flour to Enhance Omega- 3 and Omega-6 Fatty Acids Contents in Weaning Food

    NASA Astrophysics Data System (ADS)

    Marsyha, D. D.; Wijayanti, H. S.; Nuryanto; Anjani, G.

    2018-02-01

    The case of undernourished children in Grobogan District (15.3%) is caused by children nutrients intake less than the Recommendation Dietary Allowance (RDA). To enhance children nutrients intake, be required formulation of weaning food using high-nutrient local food such as golden apple snail (Pomacea canaliculata). Golden apple snail flour contains high contents of zinc, iron, omega-3 and omega-6 fatty acids. This study aims to analyze the effect of golden apple snail flour substitution on nutrients content and organoleptic properties of weaning food (baby porridge). This is an experimental research by substitution of golden apple snail flour in the making of weaning food with four treatments of substitution (0%, 5%, 10%, 15%). Substitution of golden apple snails flour could affect the nutrient content levels of fat, zinc, iron (p=0.0001), carbohydrate (p=0.011), water (p=0.003), ash (p=0.001), omega-3 and omega-6 fatty acids. Whereas, it could not affect the content of energy (p=0.678), protein (p=0.129) and fiber (p=0.482). Furthermore, the substitution could affect the organoleptic properties include color, texture and taste (p=0.0001) while not for the aroma (p=0.798). Based on nutrient content analysis, substitution of golden apple snail flour could enhance the zinc, iron, omega-3 and omega-6 fatty acids contents of weaning food.

  7. Changes in nutrient stoichiometry, elemental homeostasis and growth rate of aquatic litter-associated fungi in response to inorganic nutrient supply.

    PubMed

    Gulis, Vladislav; Kuehn, Kevin A; Schoettle, Louie N; Leach, Desiree; Benstead, Jonathan P; Rosemond, Amy D

    2017-12-01

    Aquatic fungi mediate important energy and nutrient transfers in freshwater ecosystems, a role potentially altered by widespread eutrophication. We studied the effects of dissolved nitrogen (N) and phosphorus (P) concentrations and ratios on fungal stoichiometry, elemental homeostasis, nutrient uptake and growth rate in two experiments that used (1) liquid media and a relatively recalcitrant carbon (C) source and (2) fungi grown on leaf litter in microcosms. Two monospecific fungal cultures and a multi-species assemblage were assessed in each experiment. Combining a radioactive tracer to estimate fungal production (C accrual) with N and P uptake measurements provided an ecologically relevant estimate of mean fungal C:N:P of 107:9:1 in litter-associated fungi, similar to the 92:9:1 obtained from liquid cultures. Aquatic fungi were found to be relatively homeostatic with respect to their C:N ratio (~11:1), but non-homeostatic with respect to C:P and N:P. Dissolved N greatly affected fungal growth rate and production, with little effect on C:nutrient stoichiometry. Conversely, dissolved P did not affect fungal growth and production but controlled biomass C:P and N:P, probably via luxury P uptake and storage. The ability of fungi to immobilize and store excess P may alter nutrient flow through aquatic food webs and affect ecosystem functioning.

  8. Dynamics of microorganism populations in recirculating nutrient solutions

    NASA Technical Reports Server (NTRS)

    Strayer, R. F.

    1994-01-01

    This overview covers the basic microbial ecology of recirculating hydroponic solutions. Examples from NASA and Soviet Controlled Ecological Life Support Systems (CELSS) tests and the commercial hydroponic industry will be used. The sources of microorganisms in nutrient solutions include air, water, seeds, plant containers and plumbing, biological vectors, and personnel. Microbial fates include growth, death, and emigration. Important microbial habitats within nutrient delivery systems are root surfaces, hardware surfaces (biofilms), and solution suspension. Numbers of bacteria on root surfaces usually exceed those from the other habitats by several orders of magnitude. Gram negative bacteria dominate the microflora with fungal counts usually much lower. Trends typically show a decrease in counts with increasing time unless stressed plants increase root exudates. Important microbial activities include carbon mineralization and nitrogen transformations. Important detrimental interactions include competition with plants, and human and plant pathogenesis.

  9. Factors affecting nutrient trends in major rivers of the Chesapeake Bay Watershed

    USGS Publications Warehouse

    Sprague, Lori A.; Langland, M.J.; Yochum, S.E.; Edwards, R.E.; Blomquist, J.D.; Phillips, S.W.; Shenk, G.W.; Preston, S.D.

    2000-01-01

    Trends in nutrient loads and flow-adjusted concentrations in the major rivers entering Chesapeake Bay were computed on the basis of water-quality data collected between 1985 and 1998 at 29 monitoring stations in the Susquehanna, Potomac, James, Rappahannock, York, Patuxent, and Choptank River Basins. Two computer models?the Chesapeake Bay Watershed Model (WSM) and the U.S. Geological Survey?s 'Spatially Referenced Regressions on Watershed attributes' (SPARROW) Model?were used to help explain the major factors affecting the trends. Results from WSM simulations provided information on temporal changes in contributions from major nutrient sources, and results from SPARROW model simulations provided spatial detail on the distribution of nutrient yields in these basins. Additional data on nutrient sources, basin characteristics, implementation of management practices, and ground-water inputs to surface water were analyzed to help explain the trends. The major factors affecting the trends were changes in nutrient sources and natural variations in streamflow. The dominant source of nitrogen and phosphorus from 1985 to 1998 in six of the seven tributary basins to Chesapeake Bay was determined to be agriculture. Because of the predominance of agricultural inputs, changes in agricultural nutrient sources such as manure and fertilizer, combined with decreases in agricultural acreage and implementation of best management practices (BMPs), had the greatest impact on the trends in flow-adjusted nutrient concentrations. Urban acreage and population, however, were noted to be increasing throughout the Chesapeake Bay Watershed, and as a result, delivered loads of nutrients from urban areas increased during the study period. Overall, agricultural nutrient management, in combination with load decreases from point sources due to facility upgrades and the phosphate detergent ban, led to downward trends in flow-adjusted nutrient concentrations atmany of the monitoring stations in the watershed. The loads of nutrients, however, were not reduced significantly at most of the monitoring stations. This is due primarily to higher streamflow in the latter years of the monitoring period, which led to higher loading in those years.Results of this study indicate a need for more detailed information on BMP effectiveness under a full range of hydrologic conditions and in different areas of the watershed; an internally consistent fertilizer data set; greater consideration of the effects of watershed processes on nutrient transport; a refinement of current modeling efforts; and an expansion of the non-tidal monitoring network in the Chesapeake Bay Watershed.

  10. A Bayesian changepoint-threshold model to examine the effect of TMDL implementation on the flow-nitrogen concentration relationship in the Neuse River basin.

    PubMed

    Alameddine, Ibrahim; Qian, Song S; Reckhow, Kenneth H

    2011-01-01

    In-stream nutrient concentrations are well known to exhibit a strong relationship with river flow. The use of flow measurements to predict nutrient concentrations and subsequently nutrient loads is common in water quality modeling. Nevertheless, most adopted models assume that the relationship between flow and concentration is fixed across time as well as across different flow regimes. In this study, we developed a Bayesian changepoint-threshold model that relaxes these constraints and allows for the identification and quantification of any changes in the underlying flow-concentration relationship across time. The results from our study support the occurrence of a changepoint in time around the year 1999, which coincided with the period of implementing nitrogen control measures as part of the TMDL program developed for the Neuse Estuary in North Carolina. The occurrence of the changepoint challenges the underlying assumption of temporal invariance in the flow-concentrations relationship. The model results also point towards a transition in the river nitrogen delivery system from a point source dominated loading system towards a more complicated nonlinear system, where non-point source nutrient delivery plays a major role. Moreover, we use the developed model to assess the effectiveness of the nitrogen reduction measures in achieving a 30% drop in loading. The results indicate that while there is a strong evidence of a load reduction, there still remains a high level of uncertainty associated with the mean nitrogen load reduction. We show that the level of uncertainty around the estimated load reduction is not random but is flow related. Copyright © 2010 Elsevier Ltd. All rights reserved.

  11. Nutrient contributions to the Santa Barbara Channel, California, from the ephemeral Santa Clara River

    USGS Publications Warehouse

    Warrick, J.A.; Washburn, L.; Brzezinski, Mark A.; Siegel, D.A.

    2005-01-01

    The Santa Clara River delivers nutrient rich runoff to the eastern Santa Barbara Channel during brief (???1-3 day) episodic events. Using both river and oceanographic measurements, we evaluate river loading and dispersal of dissolved macronutrients (silicate, inorganic N and P) and comment on the biological implications of these nutrient contributions. Both river and ocean observations suggest that river nutrient concentrations are inversely related to river flow rates. Land use is suggested to influence these concentrations, since runoff from a subwatershed with substantial agriculture and urban areas had much higher nitrate than runoff from a wooded subwatershed. During runoff events, river nutrients were observed to conservatively mix into the buoyant, surface plume immediately seaward of the Santa Clara River mouth. Dispersal of these river nutrients extended 10s of km into the channel. Growth of phytoplankton and nutrient uptake was low during our observations (1-3 days following runoff), presumably due to the very low light levels resulting from high turbidity. However, nutrient quality of runoff (Si:N:P = 16:5:1) was found to be significantly different than upwelling inputs (13:10:1), which may influence different algal responses once sediments settle. Evaluation of total river nitrate loads suggests that most of the annual river nutrient fluxes to the ocean occur during the brief winter flooding events. Wet winters (such as El Nin??o) contribute nutrients at rates approximately an order-of-magnitude greater than "average" winters. Although total river nitrate delivery is considerably less than that supplied by upwelling, the timing and location of these types of events are very different, with river discharge (upwelling) occurring predominantly in the winter (summer) and in the eastern (western) channel. ?? 2004 Elsevier Ltd. All rights reserved.

  12. Dietary potassium diformate did not affect growth and survival but did reduce nutrient digestibility of Pacific white shrimp cultured under clean water conditions

    USDA-ARS?s Scientific Manuscript database

    This study investigated the effect of a dietary supplement potassium diformate (PDF) on growth performance, survival and nutrient digestibility of Pacific white shrimp cultured under clean water conditions. We found that weight gain was not significantly (P>0.05) affected by the different levels of ...

  13. Stream nutrient enrichment has a greater effect on coarse than on fine benthic organic matter

    Treesearch

    Cynthia J. Tant; Amy D. Rosemond; Matthew R. First

    2013-01-01

    Nutrient enrichment affects bacteria and fungi associated with detritus, but little is known about how biota associated with different size fractions of organic matter respond to nutrients. Bacteria dominate on fine (1 mm) fractions, which are used by different groups of detritivores. We measured the effect of experimental...

  14. Nutrient Exchange through Hyphae in Intercropping Systems Affects Yields

    ERIC Educational Resources Information Center

    Thun, Tim Von

    2013-01-01

    Arbuscular mycorrhizae fungi (AMF) play a large role in the current understanding of the soil ecosystem. They increase nutrient and water uptake, improve soil structure, and form complex hyphal networks that transfer nutrients between plants within an ecosystem. Factors such as species present, the physiological balance between the plants in the…

  15. Nutrient losses from Fall and Winter-applied manure: Effects of timing and soil temperature

    USDA-ARS?s Scientific Manuscript database

    Soil temperature is a major environmental factor that affects both the infiltration of meltwater and precipitation, and nutrient cycling. The objectives of this study were to determine nutrient losses in runoff and leachate from fall and winter-applied dairy manure based on the soil temperature at t...

  16. PHYSICAL AND BIOLOGICAL CONTROLS ON DISSOLVED OXYGEN DYNAMICS IN A NORTHEASTERN GULF OF MEXICO ESTUARY

    EPA Science Inventory

    Nutrient over-enrichment is one of the most often cited causes of 305b impairment in coastal waters. Excessive nutrients affect designated uses of the nation's aquatic resources, and pose risks to human health and the environment. The process of developing nutrient criteria for e...

  17. Changes in soil nutrients after 10 years of cattle manure and swine effluent application

    USDA-ARS?s Scientific Manuscript database

    Application of cattle manure and swine effluent to cropland builds nutrient pools, affects soil quality, and increases crop productivity. The objective of the present study was to evaluate the rate of change in soil nutrient concentration and soil chemical properties due to cattle manure and swine e...

  18. Streamside Management Zones Affect Movement of Silvicultural Nitrogen and Phosphorus Fertilizers to Piedmont Streams

    Treesearch

    Joseph M. Secoges; Wallace M. Aust; John R. Seiler; C. Andrew Dolloff; William A. Lakel

    2013-01-01

    Forestry best management practices (BMP) recommendations for streamside management zones (SMZs) are based on limited data regarding SMZ width, partial harvests, and nutrient movements after forest fertilization. Agricultural fertilization is commonly linked to increased stream nutrients. However, less is known about effectiveness of SMZ options for controlling nutrient...

  19. A specific multi-nutrient formulation enhances M1 muscarinic acetylcholine receptor responses in vitro.

    PubMed

    Savelkoul, Paul J M; Janickova, Helena; Kuipers, Almar A M; Hageman, Robert J J; Kamphuis, Patrick J; Dolezal, Vladimir; Broersen, Laus M

    2012-02-01

    Recent evidence indicates that supplementation with a specific combination of nutrients may affect cell membrane synthesis and composition. To investigate whether such nutrients may also modify the physical properties of membranes, and affect membrane-bound processes involved in signal transduction pathways, we studied the effects of nutrient supplementation on G protein-coupled receptor activation in vitro. In particular, we investigated muscarinic receptors, which are important for the progression of memory deterioration and pathology of Alzheimer's disease. Nerve growth factor differentiated pheochromocytoma cells that were supplemented with specific combinations of nutrients showed enhanced responses to muscarinic receptor agonists in a membrane potential assay. The largest effects were obtained with a combination of nutrients known as Fortasyn™ Connect, comprising docosahexaenoic acid, eicosapentaenoic acid, uridine monophosphate as a uridine source, choline, vitamin B6, vitamin B12, folic acid, phospholipids, vitamin C, vitamin E, and selenium. In subsequent experiments, it was shown that the effects of supplementation could not be attributed to single nutrients. In addition, it was shown that the agonist-induced response and the supplement-induced enhancement of the response were blocked with the muscarinic receptor antagonists atropine, telenzepine, and AF-DX 384. In order to determine whether the effects of Fortasyn™ Connect supplementation were receptor subtype specific, we investigated binding properties and activation of human muscarinic M1, M2 and M4 receptors in stably transfected Chinese hamster ovary cells after supplementation. Multi-nutrient supplementation did not change M1 receptor density in plasma membranes. However, M1 receptor-mediated G protein activation was significantly enhanced. In contrast, supplementation of M2- or M4-expressing cells did not affect receptor signaling. Taken together, these results indicate that a specific combination of nutrients acts synergistically in enhancing muscarinic M1 receptor responses, probably by facilitating receptor-mediated G protein activation. © 2011 The Authors. Journal of Neurochemistry © 2011 International Society for Neurochemistry.

  20. Nutrient availability affects floral scent much less than other floral and vegetative traits in Lithophragma bolanderi.

    PubMed

    Friberg, Magne; Waters, Mia T; Thompson, John N

    2017-09-01

    Many plant-pollinator interactions are mediated by floral scents that can vary among species, among populations within species and even among individuals within populations. This variation could be innate and unaffected by the environment, but, because many floral volatiles have amino-acid precursors, scent variation also could be affected by differences in nutrient availability among environments. In plants that have coevolved with specific pollinators, natural selection is likely to favour low phenotypic plasticity in floral scent even under different conditions of nutrient availability if particular scents or scent combinations are important for attracting local pollinators. Clonal pairs of multiple seed-families of two Lithophragma bolanderi (Saxifragaceae) populations were subjected to a high and a low nutrient treatment. These plants are pollinated primarily by host-specific Greya moths. It was evaluated how nutrient treatment affected variation in floral scent relative to other vegetative and reproductive traits. Floral scent strength (the per-flower emission rate) and composition were unaffected by nutrient treatment, but low-nutrient plants produced fewer and lighter leaves, fewer scapes and fewer flowers than high-nutrient plants. The results held in both populations, which differed greatly in the number and composition of floral scents produced. The results reveal a strong genetic component both to scent composition and emission level, and partly contrasts with the only previous study that has assessed the susceptibility of floral volatile signals to variation in the abundance of nutrients. These results, and the tight coevolutionary relationship between Lithophragma plants and their specialized Greya moth pollinators, indicate that reproductive traits important to coevolving interactions, such as the floral scent of L. bolanderi, may be locally specialized and more canalized than other traits important for plant fitness. © The Author 2017. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com

  1. Reviews and Syntheses: Effects of permafrost thaw on arctic aquatic ecosystems

    NASA Astrophysics Data System (ADS)

    Vonk, J. E.; Tank, S. E.; Bowden, W. B.; Laurion, I.; Vincent, W. F.; Alekseychik, P.; Amyot, M.; Billet, M. F.; Canário, J.; Cory, R. M.; Deshpande, B. N.; Helbig, M.; Jammet, M.; Karlsson, J.; Larouche, J.; MacMillan, G.; Rautio, M.; Anthony, K. M. Walter; Wickland, K. P.

    2015-07-01

    The Arctic is a water-rich region, with freshwater systems covering 16 % of the northern permafrost landscape. The thawing of this permafrost creates new freshwater ecosystems, while at the same time modifying the existing lakes, streams, and rivers that are impacted by thaw. Here, we describe the current state of knowledge regarding how permafrost thaw affects lentic and lotic systems, exploring the effects of both thermokarst (thawing and collapse of ice-rich permafrost) and deepening of the active layer (the surface soil layer that thaws and refreezes each year). Within thermokarst, we further differentiate between the effects of thermokarst in lowland areas, vs. that on hillslopes. For almost all of the processes that we explore, the effects of thaw vary regionally, and between lake and stream systems. Much of this regional variation is caused by differences in ground ice content, topography, soil type, and permafrost coverage. Together, these modifying variables determine the degree to which permafrost thaw manifests as thermokarst, whether thermokarst leads to slumping or the formation of thermokarst lakes, and the manner in which constituent delivery to freshwater systems is altered by thaw. Differences in thaw-enabled constituent delivery can be considerable, with these modifying variables determining, for example, the balance between delivery of particulate vs. dissolved constituents, and inorganic vs. organic materials. Changes in the composition of thaw-impacted waters, coupled with changes in lake morphology, can strongly affect the physical and optical properties of thermokarst lakes. The ecology of thaw-impacted systems is also likely to change, with thaw-impacted lakes and streams having unique microbiological communities, and showing differences in respiration, primary production, and food web structure that are largely driven by differences in sediment, dissolved organic matter and nutrient delivery. The degree to which thaw enables the delivery of dissolved vs. particulate organic matter, coupled with the composition of that organic matter and the morphology and stratification characteristics of recipient systems will play an important role in determining the balance between the release of organic matter as greenhouse gases (CO2 and CH4), its burial in sediments, and its loss downstream. The magnitude of thaw impacts on northern aquatic ecosystems is increasing, as is the prevalence of thaw-impacted lakes and streams. There is therefore an urgent need to address the key gaps in understanding in order to predict the full effects of permafrost thaw on aquatic ecosystems throughout the Arctic, and their consequential feedbacks to climate.

  2. SpaceX CRS-14 What's On Board Science Briefing

    NASA Image and Video Library

    2018-04-01

    Howard Levine, at left, chief scientist in the Utilization and Life Sciences Office at NASA's Kennedy Space Center, and Dave Reid, a project manager with Techshot, discuss continuing research on growing food in space, as the Veggie Passive Orbital Nutrient Delivery System (PONDS) experiment tests a new way to deliver nutrients to plants. PONDS is one of the experiments that will be aboard a Dragon spacecraft scheduled for liftoff from Cape Canaveral Air Force Station's Space Launch Complex 40 at 4:30 p.m. EST, on April 2, 2018. The SpaceX Falcon 9 rocket will launch the company's 14th Commercial Resupply Services mission to the space station.

  3. Muscle Contraction and Force: the Importance of an Ancillary Network, Nutrient Supply and Waste Removal

    PubMed Central

    Brüggemann, Dagmar A.; Risbo, Jens; Pierzynowski, Stefan G.; Harrison, Adrian P.

    2008-01-01

    Muscle contraction studies often focus solely on myofibres and the proteins known to be involved in the processes of sarcomere shortening and cross-bridge cycling, but skeletal muscle also comprises a very elaborate ancillary network of capillaries, which not only play a vital role in terms of nutrient delivery and waste product removal, but are also tethered to surrounding fibres by collagen ”wires”. This paper therefore addresses aspects of the ancillary network of skeletal muscle at both a microscopic and functional level in order to better understand its role holistically as a considerable contributor to force transfer within muscular tissue. PMID:19325816

  4. The impact of culture medium on the development and physiology of biofilms of Pseudomonas fluorescens formed on polyurethane paint.

    PubMed

    Crookes-Goodson, Wendy J; Bojanowski, Caitlin L; Kay, Michelle L; Lloyd, Pamela F; Blankemeier, Andrew; Hurtubise, Jennifer M; Singh, Kristi M; Barlow, Daniel E; Ladouceur, Harold D; Matt Eby, D; Johnson, Glenn R; Mirau, Peter A; Pehrsson, Pehr E; Fraser, Hamish L; Russell, John N

    2013-01-01

    Microbial biofilms cause the deterioration of polymeric coatings such as polyurethanes (PUs). In many cases, microbes have been shown to use the PU as a nutrient source. The interaction between biofilms and nutritive substrata is complex, since both the medium and the substratum can provide nutrients that affect biofilm formation and biodeterioration. Historically, studies of PU biodeterioration have monitored the planktonic cells in the medium surrounding the material, not the biofilm. This study monitored planktonic and biofilm cell counts, and biofilm morphology, in long-term growth experiments conducted with Pseudomonas fluorescens under different nutrient conditions. Nutrients affected planktonic and biofilm cell numbers differently, and neither was representative of the system as a whole. Microscopic examination of the biofilm revealed the presence of intracellular storage granules in biofilms grown in M9 but not yeast extract salts medium. These granules are indicative of nutrient limitation and/or entry into stationary phase, which may impact the biodegradative capability of the biofilm.

  5. Nitrogen addition affects leaf nutrition and photosynthesis in sugar maple in a nutrient-poor northern Vermont forest

    Treesearch

    David S. Ellsworth

    1999-01-01

    Sugar maple-dominated forest ecosystems in the northeastern U.S. have been receiving precipitation nitrogen (N) inputs of 15 -20 kg N ha1 year1 since at least the mid 1980s sustained chronic N inputs of this magnitude into nutrient-poor forest ecosystems may cause eutrophication and affect ecosystem functioning as well as...

  6. Nutrient enrichment differentially affects body sizes of primary consumers and predators in a detritus-based stream

    Treesearch

    John M. Davis; Amy D. Rosemond; Sue L. Eggert; Wyatt F. Cross; J. Bruce Wallace

    2010-01-01

    We assessed how a 5-yr nutrient enrichment affected the responses of different size classes of primary consumers and predators in a detritus-based headwater stream. We hypothesized that alterations in detritus availability because of enrichment would decrease the abundance and biomass of large-bodied consumers. In contrast, we found that 2 yr of enrichment increased...

  7. Trace element and nutrient accumulation in sunflower plants two years after the Aznalcóllar mine spill.

    PubMed

    Madejón, P; Murillo, J M; Marañón, T; Cabrera, F; Soriano, M A

    2003-05-20

    The failure of a tailing pond dam at the Aznalcóllar pyrite mine (SW Spain) in April 1998 released a toxic spill affecting approximately 4300 ha along the Agrio and Guadiamar valleys. Two years later, we have studied yield and concentration of mineral nutrients and trace elements in sunflower plants grown in the spill-affected soil, and in an adjacent unaffected soil as comparison. The study has been carried out in plants at seedling (V4) and mature (R8) stages. Shoot and root biomass of sunflower seedlings was significantly smaller in the affected soil than in the unaffected soil, but there was no significant difference at the mature stage. Oil production was greater in the spill-affected plants. We have not detected any 'fertilising' effect caused by the acid waters of the spill on the main nutrient (N, P and Ca) acquisition, as documented in 1998 for sunflower plants flooded by the spill. Sunflower plants growing in the spill-affected soil reached adequate levels of nutrients. None of the trace elements measured-As, Cd, Cu, Pb and Tl-reached levels either phytotoxic or toxic for humans or animals in seeds and the above-ground part of the spill-affected plants. We evaluate the potential use of sunflower plants for phytoremediation. The potential for phytoextraction is very low; however, it may be used for soil conservation. The production of oil (usable for industrial purposes) may add some value to this crop.

  8. The role of aquatic fungi in transformations of organic matter mediated by nutrients

    Treesearch

    Cynthia J. Tant; Amy D. Rosemond; Andrew S. Mehring; Kevin A. Kuehn; John M. Davis

    2015-01-01

    1. We assessed the key role of aquatic fungi in modifying coarse particulate organic matter (CPOM) by affecting its breakdown rate, nutrient concentration and conversion to fine particulate organic matter (FPOM). Overall, we hypothesised that fungal-mediated conditioning and breakdown of CPOM would be accelerated when nutrient concentrations are increased and tested...

  9. Decomposition and nutrient release from fresh and dried pine roots under two fertilizer regimes

    Treesearch

    Kim H. Ludovici; Lance W. Kress

    2006-01-01

    Root decomposition and nutrient release are typically estimated from dried root tissues; however, it is unlikely that roots dehydrate prior to decomposing. Soil fertility and root diameter may also affect the rate of decomposition. This study monitored mass loss and nutrient concentrations of dried and fresh roots of two size classes (

  10. Spray drying of Pomegranate Juice using maltodextrin/cyclodextrin blends as the wall material

    USDA-ARS?s Scientific Manuscript database

    Microencapsulation protects sensitive nutrients for preservation, masking flavors, or to enhance delivery. Ratios of maltodextrin and '-cyclodextrin (20:0, 19:1, and 17:3 % w/w) were dissolved in water and mixed with pomegranate juice for spray drying with inlet temperatures of 120, 140 and 160°C. ...

  11. On-The-Move Nutrient Delivery System (NDS): User Acceptability of Rotary Flow Control Version

    DTIC Science & Technology

    2009-04-01

    Following: Salt, Sodium Citrate, Monopotassium Phosphate, Sodium Benzoate , Potassium Sorbate, FD & C Yellow #5. Faslln,,, Flow Mallifold 126.00 g (4.44 oz...Fat Cholesterol Sodium 440mg Potassium Total Carbohydrate Dietary Fiber Sugars Protein Vitamin A Vitamin C Calcium lron Phosphorus 260 o Og Og Omg 18

  12. Correlation of Emulsion Structure with Cellular Uptake Behavior of Encapsulated Bioactive Nutrients: Influence of Droplet Size and Interfacial Structure.

    PubMed

    Lu, Wei; Kelly, Alan L; Maguire, Pierce; Zhang, Hongzhou; Stanton, Catherine; Miao, Song

    2016-11-16

    In this study, an in vitro Caco-2 cell culture assay was employed to evaluate the correlation between emulsion structure and cellular uptake of encapsulated β-carotene. After 4 h of incubation, an emulsion stabilized with whey protein isolate showed the highest intracellular accumulation of β-carotene (1.06 μg), followed by that stabilized with sodium caseinate (0.60 μg) and Tween 80 (0.20 μg), which are 13-, 7.5-, and 2.5-fold higher than that of free β-carotene (0.08 μg), respectively. Emulsions with small droplet size (239 ± 5 nm) showed a higher cellular uptake of β-carotene (1.56 μg) than emulsiond with large droplet size (489 ± 9 nm) (0.93 μg) (p < 0.01). The results suggested that delivery in an emulsion significantly improved the cellular uptake of β-carotene and thus potentially its bioavailability; uptake was closely correlated with the interfacial composition and droplet size of emulsions. The findings support the potential for achieving optimal controlled and targeted delivery of bioactive nutrients by structuring emulsions.

  13. Induced epidermal permeability modulates resistance and susceptibility of wheat seedlings to herbivory by Hessian fly larvae

    PubMed Central

    Williams, Christie E.; Nemacheck, Jill A.; Shukle, John T.; Subramanyam, Subhashree; Saltzmann, Kurt D.; Shukle, Richard H.

    2011-01-01

    Salivary secretions of neonate Hessian fly larvae initiate a two-way exchange of molecules with their wheat host. Changes in properties of the leaf surface allow larval effectors to enter the plant where they trigger plant processes leading to resistance and delivery of defence molecules, or susceptibility and delivery of nutrients. To increase understanding of the host plant's response, the timing and characteristics of the induced epidermal permeability were investigated. Resistant plant permeability was transient and limited in area, persisting just long enough to deliver defence molecules before gene expression and permeability reverted to pre-infestation levels. The abundance of transcripts for GDSL-motif lipase/hydrolase, thought to contribute to cuticle reorganization and increased permeability, followed the same temporal profile as permeability in resistant plants. In contrast, susceptible plants continued to increase in permeability over time until the entire crown of the plant became a nutrient sink. Permeability increased with higher infestation levels in susceptible but not in resistant plants. The ramifications of induced plant permeability on Hessian fly populations are discussed. PMID:21659664

  14. Ferritin cage for encapsulation and delivery of bioactive nutrients: From structure, property to applications.

    PubMed

    Zang, Jiachen; Chen, Hai; Zhao, Guanghua; Wang, Fudi; Ren, Fazheng

    2017-11-22

    Ferritin is a class of naturally occurring iron storage proteins, which is distributed widely in animal, plant, and bacteria. It usually consists of 24 subunits that form a hollow protein shell with high symmetry. One holoferritin molecule can store up to 4500 iron atom within its inner cavity, and it becomes apoferritin upon removal of iron from the cavity. Recently, scientists have subverted these nature functions and used reversibly self-assembled property of apoferritin cage controlled by pH for the encapsulation and delivery of bioactive nutrients or anticancer drug. In all these cases, the ferritin cages shield their cargo from the influence of external conditions and provide a controlled microenvironment. More importantly, upon encapsulation, ferritin shell greatly improved the water solubility, thermal stability, photostability, and cellular uptake activity of these small bioactive compounds. This review aims to highlight recent advances in applications of ferritin cage as a novel vehicle in the field of food science and nutrition. Future outlooks are highlighted with the aim to suggest a research line to follow for further studies.

  15. Animal pee in the sea: consumer-mediated nutrient dynamics in the world's changing oceans.

    PubMed

    Allgeier, Jacob E; Burkepile, Deron E; Layman, Craig A

    2017-06-01

    Humans have drastically altered the abundance of animals in marine ecosystems via exploitation. Reduced abundance can destabilize food webs, leading to cascading indirect effects that dramatically reorganize community structure and shift ecosystem function. However, the additional implications of these top-down changes for biogeochemical cycles via consumer-mediated nutrient dynamics (CND) are often overlooked in marine systems, particularly in coastal areas. Here, we review research that underscores the importance of this bottom-up control at local, regional, and global scales in coastal marine ecosystems, and the potential implications of anthropogenic change to fundamentally alter these processes. We focus attention on the two primary ways consumers affect nutrient dynamics, with emphasis on implications for the nutrient capacity of ecosystems: (1) the storage and retention of nutrients in biomass, and (2) the supply of nutrients via excretion and egestion. Nutrient storage in consumer biomass may be especially important in many marine ecosystems because consumers, as opposed to producers, often dominate organismal biomass. As for nutrient supply, we emphasize how consumers enhance primary production through both press and pulse dynamics. Looking forward, we explore the importance of CDN for improving theory (e.g., ecological stoichiometry, metabolic theory, and biodiversity-ecosystem function relationships), all in the context of global environmental change. Increasing research focus on CND will likely transform our perspectives on how consumers affect the functioning of marine ecosystems. © 2017 John Wiley & Sons Ltd.

  16. Elevated tropospheric ozone affects the concentration and allocation of mineral nutrients of two bamboo species.

    PubMed

    Zhuang, Minghao; Lam, Shu Kee; Li, Yingchun; Chen, Shuanglin

    2017-01-15

    The increase in tropospheric ozone (O 3 ) affects plant physiology and ecosystem processes, and consequently the cycle of nutrients. While mineral nutrients are critical for plant growth, the effect of elevated tropospheric O 3 concentration on the uptake and allocation of mineral nutrients by plants is not well understood. Using open top chambers (OTCs), we investigated the effect of elevated O 3 on calcium (Ca), magnesium (Mg) and iron (Fe) in mature bamboo species Phyllostachys edulis and Oligostachyum lubricum. Our results showed that elevated O 3 decreased the leaf biomass of P. edulis and O. lubricum by 35.1% and 26.7%, respectively, but had no significant effect on the biomass of branches, stem or root. For P. edulis, elevated O 3 increased the nutrient (Ca, Mg and Fe) concentration and allocation in leaf but reduced the concentration in other organs. In contrast, elevated O 3 increased the nutrient concentration and allocation in the branch of O. lubricum but decreased that of other organs. We also found that that P. edulis and O. lubricum responded differently to elevated O 3 in terms of nutrient (Ca, Mg and Fe) uptake and allocation. This information is critical for nutrient management and adaptation strategies for sustainable growth of P. edulis and O. lubricum under global climate change. Copyright © 2016 Elsevier B.V. All rights reserved.

  17. Cumulative effects of suspended sediments, organic nutrients and temperature stress on early life history stages of the coral Acropora tenuis.

    PubMed

    Humanes, Adriana; Ricardo, Gerard F; Willis, Bette L; Fabricius, Katharina E; Negri, Andrew P

    2017-03-10

    Coral reproduction is vulnerable to both declining water quality and warming temperatures, with simultaneous exposures likely compounding the negative impact of each stressor. We investigated how early life processes of the coral Acropora tenuis respond to increasing levels of suspended sediments in combination with temperature or organic nutrients. Fertilization success and embryo development were more sensitive to suspended sediments than to high temperatures or nutrient enrichment, while larval development (after acquisition of cilia) and settlement success were predominantly affected by thermal stress. Fertilization success was reduced 80% by suspended sediments, and up to 24% by temperature, while the addition of nutrients to suspended sediments had no further impact. Larval survivorship was unaffected by any of these treatments. However, settlement success of larvae developing from treatment-exposed embryos was negatively affected by all three stressors (e.g. up to 55% by suspended sediments), while exposure only during later larval stages predominantly responded to temperature stress. Environmentally relevant levels of suspended sediments and temperature had the greatest impacts, affecting more processes than the combined impacts of sediments and nutrients. These results suggest that management strategies to maintain suspended sediments at low concentrations during coral spawning events will benefit coral recruitment, especially with warming climate.

  18. Cumulative effects of suspended sediments, organic nutrients and temperature stress on early life history stages of the coral Acropora tenuis

    NASA Astrophysics Data System (ADS)

    Humanes, Adriana; Ricardo, Gerard F.; Willis, Bette L.; Fabricius, Katharina E.; Negri, Andrew P.

    2017-03-01

    Coral reproduction is vulnerable to both declining water quality and warming temperatures, with simultaneous exposures likely compounding the negative impact of each stressor. We investigated how early life processes of the coral Acropora tenuis respond to increasing levels of suspended sediments in combination with temperature or organic nutrients. Fertilization success and embryo development were more sensitive to suspended sediments than to high temperatures or nutrient enrichment, while larval development (after acquisition of cilia) and settlement success were predominantly affected by thermal stress. Fertilization success was reduced 80% by suspended sediments, and up to 24% by temperature, while the addition of nutrients to suspended sediments had no further impact. Larval survivorship was unaffected by any of these treatments. However, settlement success of larvae developing from treatment-exposed embryos was negatively affected by all three stressors (e.g. up to 55% by suspended sediments), while exposure only during later larval stages predominantly responded to temperature stress. Environmentally relevant levels of suspended sediments and temperature had the greatest impacts, affecting more processes than the combined impacts of sediments and nutrients. These results suggest that management strategies to maintain suspended sediments at low concentrations during coral spawning events will benefit coral recruitment, especially with warming climate.

  19. Methods and apparatuses for reagent delivery, reactive barrier formation, and pest control

    DOEpatents

    Gilmore, Tyler [Pasco, WA; Kaplan, Daniel I [Aiken, SC; Last, George [Richland, WA

    2002-07-09

    A reagent delivery method includes positioning reagent delivery tubes in contact with soil. The tubes can include a wall that is permeable to a soil-modifying reagent. The method further includes supplying the reagent in the tubes, diffusing the reagent through the permeable wall and into the soil, and chemically modifying a selected component of the soil using the reagent. The tubes can be in subsurface contact with soil, including groundwater, and can be placed with directional drilling equipment independent of groundwater well casings. The soil-modifying reagent includes a variety of gases, liquids, colloids, and adsorbents that may be reactive or non-reactive with soil components. The method may be used inter alia to form reactive barriers, control pests, and enhance soil nutrients for microbes and plants.

  20. Searching for a life history approach to salmon escapement management

    USGS Publications Warehouse

    Knudsen, E.E.; Symmes, E.W.; Margraf, F.J.

    2003-01-01

    A number of Pacific salmon populations have already been lost and many others throughout the range are in various states of decline. Recent research has documented that Pacific salmon carcasses serve as a key delivery vector of marine-derived nutrients into the freshwater portions of their ecosystems. This nutrient supply plays a critical biological feedback role in salmon sustainability by supporting juvenile salmon production. We first demonstrate how nutrient feedback potential to juvenile production may be unaccounted for in spawner-recruit models of populations under long-term exploitation. We then present a heuristic, life history-based, spreadsheet survival model that incorporates salmon carcass-driven nutrient feedback to the freshwater components of the salmon ecosystem. The productivity of a hypothetical coho salmon population was simulated using rates from the literature for survival from spawner to egg, egg to fry, fry to smolt, and smolt to adult. The effects of climate variation and nutrient feedback on survival were incorporated, as were density-dependent effects of the numbers of spawners and fry on freshwater survival of eggs and juveniles. The unexploited equilibrium population was subjected to 100 years of 20, 40, 60, and 80% harvest. Each harvest scenario greater than 20% brought the population to a reduced steady state, regardless of generous compensatory survival at low population sizes. Increasing harvest reduced the positive effects of nutrient contributions to population growth. Salmon researchers should further explore this modeling approach for establishing escapement goals. Given the importance of nutrient feedback, managers should strive for generous escapements that support nutrient rebuilding, as well as egg deposition, to ensure strong future salmon production.

  1. Effect of algal blooms on retention of N, Si and P in Europe's largest coastal lagoon

    NASA Astrophysics Data System (ADS)

    Vybernaite-Lubiene, I.; Zilius, M.; Giordani, G.; Petkuviene, J.; Vaiciute, D.; Bukaveckas, P. A.; Bartoli, M.

    2017-07-01

    Nutrient fluxes from land to sea are regulated by climatic factors governing hydrologic loading rates (e.g., storm events, snowmelt) and by internal processes within estuaries that affect nutrient transformation and retention. We compared monthly input and output fluxes of N, Si, and P at the entrance and exit of the hypereutrophic Curonian Lagoon to better understand how seasonal changes in the stoichiometry of nutrient inputs and the occurrence of algal blooms affected nutrient retention within the lagoon. Nutrient ratios were indicative of increasing Si and N limitation during the growing season, and these were associated with a shift from a diatom-based to a cyanobacteria dominated phytoplankton community. The estuary was a net sink for dissolved nutrients, but we observed large interannual difference in the overall retention of N and P. The occurrence of a large cyanobacteria bloom in 2012 was associated with increased export of particulate matter to the Baltic Sea resulting in a net surplus of P export. Bloom conditions mobilized P from sediments and resulted in a shift from net retention to net export for the lagoon. The findings of our study illustrate how changes in nutrient loading ratios influence phytoplankton community composition, which in turn alters the source-sink status of the estuary.

  2. Control of water and nutrients using a porous tube - A method for growing plants in space

    NASA Technical Reports Server (NTRS)

    Dreschel, Thomas W.; Sager, John C.

    1989-01-01

    A plant nutrient delivery system that uses a microporous, hydrophilic tube was developed with potential application for crop production in the microgravity of space. The tube contains a nutrient solution and delivers it to the roots. Pumps attached to the tubing create a very small suction that holds the solution within the tube. This system was used to grow wheat for 107 d in a controlled environment at suctions of 0.40, 1.48, or 2.58 kPa. The water absorbed through the pores of the tube by baby diaper sections decreased as suction increased. Correspondingly, final plant biomass, seed number, and spikelet number also tended to decrease as suction increased. The reduced yield at higher suction suggests that the plants experienced water stress, although all suctions were below those typical of soils at field capacity.

  3. Influences of Geomorphic Complexity and Rehabilitation on Nutrient Uptake in an Urban Stream

    NASA Astrophysics Data System (ADS)

    Mueller, J. S.; Baker, D. W.; Bledsoe, B. P.

    2006-12-01

    Headwater streams, which are highly vulnerable to anthropogenic impacts associated with land use change, have large surface-to-volume ratios that favor retention and removal of nitrogen. We describe a study focused on how geomorphic complexity is related to nutrient retention in impacted and restored headwater streams along a gradient of human land use. A key element of the study is a detailed protocol for characterizing the spatial distribution of physical habitat units composed of relatively distinct combinations of flow hydraulics and textural facies. We are using the detailed physical characterization and nutrient injections in paired segments of a Colorado Front Range urban stream to examine associations among geomorphic complexity, nitrogen uptake, and the degree and style of channel rehabilitation. The results of the study have implications for the viability of stream rehabilitation as a tool for reducing N delivery to downstream aquatic systems that are vulnerable to eutrophication.

  4. Nanoscale semiconducting silicon as a nutritional food additive

    NASA Astrophysics Data System (ADS)

    Canham, L. T.

    2007-05-01

    Very high surface area silicon powders can be realized by high energy milling or electrochemical etching techniques. Such nanoscale silicon structures, whilst biodegradable in the human gastrointestinal tract, are shown to be remarkably stable in most foodstuffs and beverages. The potential for using silicon to improve the shelf life and bioavailability of specific nutrients in functional foods is highlighted. Published drug delivery data implies that the nanoentrapment of hydrophobic nutrients will significantly improve their dissolution kinetics, through a combined effect of nanostructuring and solid state modification. Nutrients loaded to date include vitamins, fish oils, lycopene and coenzyme Q10. In addition, there is growing published evidence that optimized release of orthosilicic acid, the biodegradation product of semiconducting silicon in the gut, offers beneficial effects with regard bone health. The utility of nanoscale silicon in the nutritional field shows early promise and is worthy of much further study.

  5. The role of topographic structure and soil macrofauna presence at spoil heaps during spontaneous succession.

    NASA Astrophysics Data System (ADS)

    Walmsley, Alena; Vachová, Pavla; Vach, Marek

    2016-04-01

    This research was investigating whether topographic features, which determine soil nutrient and moisture distribution, in combination with soil fauna (wireworm and earthworm) presence, affect plant community composition at a spontaneously revegetated post mining area with an undulating surface. Two sites of different age with 3 types of topographic features were selected, soil moisture and nutrient content were measured, plant community composition and soil macrofauna community was sampled at each position. Wireworms were present at all positions and were most abundant at bottoms of waves at the younger site; their presence was correlated with several plant species, but the direction of the interaction isn't clear. Earthworms were only present at the older site and had highest abundance at flat sections. Earthworm presence affected the amount of nitrogen in soil - the most nitrogen content was at the site with highest earthworm density and was followed by higher diversity of plant community. The plant community composition was generally correlated with plant available nutrient content - especially P and N. We infer that topographic features affect nutrient and soil fauna distribution, which consequently influences plant community composition.

  6. Global-scale patterns of nutrient density and partitioning in forests in relation to climate.

    PubMed

    Zhang, Kerong; Song, Conghe; Zhang, Yulong; Dang, Haishan; Cheng, Xiaoli; Zhang, Quanfa

    2018-01-01

    Knowledge of nutrient storage and partitioning in forests is imperative for ecosystem models and ecological theory. Whether the nutrients (N, P, K, Ca, and Mg) stored in forest biomass and their partitioning patterns vary systematically across climatic gradients remains unknown. Here, we explored the global-scale patterns of nutrient density and partitioning using a newly compiled dataset including 372 forest stands. We found that temperature and precipitation were key factors driving the nutrients stored in living biomass of forests at global scale. The N, K, and Mg stored in living biomass tended to be greater in increasingly warm climates. The mean biomass N density was 577.0, 530.4, 513.2, and 336.7 kg/ha for tropical, subtropical, temperate, and boreal forests, respectively. Around 76% of the variation in biomass N density could be accounted by the empirical model combining biomass density, phylogeny (i.e., angiosperm, gymnosperm), and the interaction of mean annual temperature and precipitation. Climate, stand age, and biomass density significantly affected nutrients partitioning at forest community level. The fractional distribution of nutrients to roots decreased significantly with temperature, suggesting that forests in cold climates allocate greater nutrients to roots. Gymnosperm forests tended to allocate more nutrients to leaves as compared with angiosperm forests, whereas the angiosperm forests distributed more nutrients in stems. The nutrient-based Root:Shoot ratios (R:S), averaged 0.30 for R:S N , 0.36 for R:S P , 0.32 for R:S K , 0.27 for R:S Ca , and 0.35 for R:S Mg , respectively. The scaling exponents of the relationships describing root nutrients as a function of shoot nutrients were more than 1.0, suggesting that as nutrient allocated to shoot increases, nutrient allocated to roots increases faster than linearly with nutrient in shoot. Soil type significantly affected the total N, P, K, Ca, and Mg stored in living biomass of forests, and the Acrisols group displayed the lowest P, K, Ca, and Mg. © 2017 John Wiley & Sons Ltd.

  7. Effects of climate change on the delivery of soil-mediated ecosystem services within the primary sector in temperate ecosystems: a review and New Zealand case study.

    PubMed

    Orwin, Kate H; Stevenson, Bryan A; Smaill, Simeon J; Kirschbaum, Miko U F; Dickie, Ian A; Clothier, Brent E; Garrett, Loretta G; van der Weerden, Tony J; Beare, Michael H; Curtin, Denis; de Klein, Cecile A M; Dodd, Michael B; Gentile, Roberta; Hedley, Carolyn; Mullan, Brett; Shepherd, Mark; Wakelin, Steven A; Bell, Nigel; Bowatte, Saman; Davis, Murray R; Dominati, Estelle; O'Callaghan, Maureen; Parfitt, Roger L; Thomas, Steve M

    2015-08-01

    Future human well-being under climate change depends on the ongoing delivery of food, fibre and wood from the land-based primary sector. The ability to deliver these provisioning services depends on soil-based ecosystem services (e.g. carbon, nutrient and water cycling and storage), yet we lack an in-depth understanding of the likely response of soil-based ecosystem services to climate change. We review the current knowledge on this topic for temperate ecosystems, focusing on mechanisms that are likely to underpin differences in climate change responses between four primary sector systems: cropping, intensive grazing, extensive grazing and plantation forestry. We then illustrate how our findings can be applied to assess service delivery under climate change in a specific region, using New Zealand as an example system. Differences in the climate change responses of carbon and nutrient-related services between systems will largely be driven by whether they are reliant on externally added or internally cycled nutrients, the extent to which plant communities could influence responses, and variation in vulnerability to erosion. The ability of soils to regulate water under climate change will mostly be driven by changes in rainfall, but can be influenced by different primary sector systems' vulnerability to soil water repellency and differences in evapotranspiration rates. These changes in regulating services resulted in different potentials for increased biomass production across systems, with intensively managed systems being the most likely to benefit from climate change. Quantitative prediction of net effects of climate change on soil ecosystem services remains a challenge, in part due to knowledge gaps, but also due to the complex interactions between different aspects of climate change. Despite this challenge, it is critical to gain the information required to make such predictions as robust as possible given the fundamental role of soils in supporting human well-being. © 2015 John Wiley & Sons Ltd.

  8. Competition for nutrients and light: testing advances in resource competition with a natural phytoplankton community.

    PubMed

    Burson, Amanda; Stomp, Maayke; Greenwell, Emma; Grosse, Julia; Huisman, Jef

    2018-05-01

    A key challenge in ecology is to understand how nutrients and light affect the biodiversity and community structure of phytoplankton and plant communities. According to resource competition models, ratios of limiting nutrients are major determinants of species composition. At high nutrient levels, however, species interactions may shift to competition for light, which might make nutrient ratios less relevant. The "nutrient-load hypothesis" merges these two perspectives, by extending the classic model of competition for two nutrients to include competition for light. Here, we test five key predictions of the nutrient-load hypothesis using multispecies competition experiments. A marine phytoplankton community sampled from the North Sea was inoculated in laboratory chemostats provided with different nitrogen (N) and phosphorus (P) loads to induce either single resource limitation or co-limitation of N, P, and light. Four of the five predictions were validated by the experiments. In particular, different resource limitations favored the dominance of different species. Increasing nutrient loads caused changes in phytoplankton species composition, even if the N:P ratio of the nutrient loads remained constant, by shifting the species interactions from competition for nutrients to competition for light. In all treatments, small species became dominant whereas larger species were competitively excluded, supporting the common view that small cell size provides a competitive advantage under resource-limited conditions. Contrary to expectation, all treatments led to coexistence of diatoms, cyanobacteria and green algae, resulting in a higher diversity of species than predicted by theory. Because the coexisting species comprised three phyla with different photosynthetic pigments, we speculate that niche differentiation in the light spectrum might play a role. Our results show that mechanistic resource competition models that integrate nutrient-based and light-based approaches provide an important step forward to understand and predict how changing nutrient loads affect community composition. © 2018 The Authors Ecology published by Wiley Periodicals, Inc. on behalf of Ecological Society of America.

  9. Marine microorganisms and global nutrient cycles

    NASA Astrophysics Data System (ADS)

    Arrigo, Kevin R.

    2005-09-01

    The way that nutrients cycle through atmospheric, terrestrial, oceanic and associated biotic reservoirs can constrain rates of biological production and help structure ecosystems on land and in the sea. On a global scale, cycling of nutrients also affects the concentration of atmospheric carbon dioxide. Because of their capacity for rapid growth, marine microorganisms are a major component of global nutrient cycles. Understanding what controls their distributions and their diverse suite of nutrient transformations is a major challenge facing contemporary biological oceanographers. What is emerging is an appreciation of the previously unknown degree of complexity within the marine microbial community.

  10. Mitochondrial Dynamics Mediated by Mitofusin 1 Is Required for POMC Neuron Glucose-Sensing and Insulin Release Control.

    PubMed

    Ramírez, Sara; Gómez-Valadés, Alicia G; Schneeberger, Marc; Varela, Luis; Haddad-Tóvolli, Roberta; Altirriba, Jordi; Noguera, Eduard; Drougard, Anne; Flores-Martínez, Álvaro; Imbernón, Mónica; Chivite, Iñigo; Pozo, Macarena; Vidal-Itriago, Andrés; Garcia, Ainhoa; Cervantes, Sara; Gasa, Rosa; Nogueiras, Ruben; Gama-Pérez, Pau; Garcia-Roves, Pablo M; Cano, David A; Knauf, Claude; Servitja, Joan-Marc; Horvath, Tamas L; Gomis, Ramon; Zorzano, Antonio; Claret, Marc

    2017-06-06

    Proopiomelanocortin (POMC) neurons are critical sensors of nutrient availability implicated in energy balance and glucose metabolism control. However, the precise mechanisms underlying nutrient sensing in POMC neurons remain incompletely understood. We show that mitochondrial dynamics mediated by Mitofusin 1 (MFN1) in POMC neurons couple nutrient sensing with systemic glucose metabolism. Mice lacking MFN1 in POMC neurons exhibited defective mitochondrial architecture remodeling and attenuated hypothalamic gene expression programs during the fast-to-fed transition. This loss of mitochondrial flexibility in POMC neurons bidirectionally altered glucose sensing, causing abnormal glucose homeostasis due to defective insulin secretion by pancreatic β cells. Fed mice lacking MFN1 in POMC neurons displayed enhanced hypothalamic mitochondrial oxygen flux and reactive oxygen species generation. Central delivery of antioxidants was able to normalize the phenotype. Collectively, our data posit MFN1-mediated mitochondrial dynamics in POMC neurons as an intrinsic nutrient-sensing mechanism and unveil an unrecognized link between this subset of neurons and insulin release. Copyright © 2017 Elsevier Inc. All rights reserved.

  11. The effect of resource quantity and resource stoichiometry on microbial carbon-use-efficiency

    USGS Publications Warehouse

    Kleiblinger, K.M.; Hall, E.K.; Wanek, W.; Szukics, U.; Hämmerle, I.; Ellersdorfer, G.; Böck, S.; Strauss, J.; Sterflinger, K.; Richter, A.; Zechmeister-Boltenstern, S.

    2010-01-01

    The carbon-use-efficiency (CUE) of microorganisms is an important parameter in determining ecosystem-level carbon (C) cycling; however, little is known about how variance in resources affects microbial CUE. To elucidate how resource quantity and resource stoichiometry affect microbial CUE, we cultured four microorganisms - two fungi (Aspergillus nidulans and Trichoderma harzianum) and two bacteria (Pectobacterium carotovorum and Verrucomicrobium spinosum) - under 12 unique C, nitrogen (N) and phosphorus (P) ratios. Whereas the CUE of A. nidulans was strongly affected by C, bacterial CUE was more strongly affected by mineral nutrients (N and P). Specifically, CUE in P. carotovorum was positively correlated with P, while CUE of V. spinosum primarily depended on N. This resulted in a positive relationship between fungal CUE and resource C : nutrient stoichiometry and a negative relationship between bacterial CUE and resource C : nutrient stoichiometry. The difference in the direction of the relationship between CUE and C : nutrient for fungi vs. bacteria was consistent with differences in biomass stoichiometry and suggested that fungi have a higher C demand than bacteria. These results suggest that the links between biomass stoichiometry, resource demand and CUE may provide a mechanism for commonly observed temporal and spatial patterns in microbial community structure and function in natural habitats.

  12. A Regional Assessment of the Effects of Conservation Practices on In-stream Water Quality

    NASA Astrophysics Data System (ADS)

    Garcia, A. M.; Alexander, R. B.; Arnold, J.; Norfleet, L.; Robertson, D. M.; White, M.

    2011-12-01

    The Conservation Effects Assessment Program (CEAP), initiated by USDA Natural Resources Conservation Service (NRCS), has the goal of quantifying the environmental benefits of agricultural conservation practices. As part of this effort, detailed farmer surveys were compiled to document the adoption of conservation practices. Survey data showed that up to 38 percent of cropland in the Upper Mississippi River basin is managed to reduce sediment, nutrient and pesticide loads from agricultural activities. The broader effects of these practices on downstream water quality are challenging to quantify. The USDA-NRCS recently reported results of a study that combined farmer surveys with process-based models to deduce the effect of conservation practices on sediment and chemical loads in farm runoff and downstream waters. As a follow-up collaboration, USGS and USDA scientists conducted a semi-empirical assessment of the same suite of practices using the USGS SPARROW (SPAtially Referenced Regression On Watershed attributes) modeling framework. SPARROW is a hybrid statistical and mechanistic stream water quality model of annual conditions that has been used extensively in studies of nutrient sources and delivery. In this assessment, the USDA simulations of the effects of conservation practices on loads in farm runoff were used as an explanatory variable (i.e., change in farm loads per unit area) in a component of an existing a SPARROW model of the Upper Midwest. The model was then re-calibrated and tested to determine whether the USDA estimate of conservation adoption intensity explained a statistically significant proportion of the spatial variability in stream nutrient loads in the Upper Mississippi River basin. The results showed that the suite of conservation practices that NRCS has catalogued as complete nutrient and sediment management are a statistically significant feature in the Midwestern landscape associated with phosphorous runoff and delivery to downstream waters. Effects on the delivery of nitrogen will be also be studied. Estimates of the magnitude of this effect using SPARROW indicated that phosphorus load reductions ranged from about 2 - 38% for various spatial scales. This is less than reported by the USDA CEAP simulations, which ranged from 15 - 49%. Nevertheless, the results indicated that conservation practices play a significant role in reducing phosphorus pollution from agricultural activities to downstream receiving water bodies.

  13. Select nutrients, progesterone, and interferon tau affect conceptus metabolism and development

    PubMed Central

    Bazer, Fuller W; Kim, Jingyoung; Song, Gwonhwa; Ka, Hakhyun; Tekwe, Carmen D; Wu, Guoyao

    2012-01-01

    Interferon tau (IFNT), a novel multifunctional type I interferon secreted by trophectoderm, is the pregnancy recognition signal in ruminants that also has antiviral, antiproliferative, and immunomodulatory bioactivities. IFNT, with progesterone, affects availability of the metabolic substrate in the uterine lumen by inducing expression of genes for transport of select nutrients into the uterine lumen that activate mammalian target of rapamycin (mTOR) cell signaling responsible for proliferation, migration, and protein synthesis by conceptus trophectoderm. As an immunomodulatory protein, IFNT induces an anti-inflammatory state affecting metabolic events that decrease adiposity and glutamine:fructose-6-phosphate amidotransferase 1 activity, while increasing insulin sensitivity, nitric oxide production by endothelial cells, and brown adipose tissue in rats. This short review focuses on effects of IFNT and progesterone affecting transport of select nutrients into the uterine lumen to stimulate mTOR cell signaling required for conceptus development, as well as effects of IFNT on the immune system and adiposity in rats with respect to its potential therapeutic value in reducing obesity. PMID:23050969

  14. Amounts of calcium and magnesium cycled and its release kinetics in a long-term no-till integrated crop-livestock system with varying grazing intensities in subtropical region

    USDA-ARS?s Scientific Manuscript database

    Under an integrated crop-livestock production system, plant and animal residues become an important nutrient stock. Grazing management could affect both plant and animal residue amount and quality, thereby influencing nutrient dynamics through modifications in nutrient release rates. The objective o...

  15. Marine fisheries declines viewed upside down: human impacts on consumer-driven nutrient recycling.

    PubMed

    Layman, Craig A; Allgeier, Jacob E; Rosemond, Amy D; Dahlgren, Craig P; Yeager, Lauren A

    2011-03-01

    We quantified how two human impacts (overfishing and habitat fragmentation) in nearshore marine ecosystems may affect ecosystem function by altering the role of fish as nutrient vectors. We empirically quantified size-specific excretion rates of one of the most abundant fishes (gray snapper, Lutjanus griseus) in The Bahamas and combined these with surveys of fish abundance to estimate population-level excretion rates. The study was conducted across gradients of two human disturbances: overfishing and ecosystem fragmentation (estuaries bisected by roads), to evaluate how each could result in reduced population-level nutrient cycling by consumers. Mean estimated N and P excretion rates for gray snapper populations were on average 456% and 541% higher, respectively, in unfished sites. Ecosystem fragmentation resulted in significant reductions of recycling rates by snapper, with degree of creek fragmentation explaining 86% and 72% of the variance in estimated excretion for dissolved N and P, respectively. Additionally, we used nutrient limitation assays and primary producer nutrient content to provide a simple example of how marine fishery declines may affect primary production. This study provides an initial step toward integrating marine fishery declines and consumer-driven nutrient recycling to more fully understand the implications of human impacts in marine ecosystems.

  16. Effects of maternal obesity on placental function and fetal development

    PubMed Central

    Howell, Kristy R.; Powell, Theresa L.

    2017-01-01

    Obesity has reached epidemic proportions and pregnancies in obese mothers have increased risk for complications including gestational diabetes, hypertensive disorders, preterm birth and caesarian section. Children born to obese mothers are at increased risk of obesity and metabolic disease and are susceptible to develop neuropsychiatric and cognitive disorders. Changes in placental function not only play a critical role in the development of pregnancy complications but may also be involved in linking maternal obesity to long-term health risks in the infant. Maternal adipokines i.e., interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), leptin and adiponectin link maternal nutritional status and adipose tissue metabolism to placental function. Adipokines and metabolic hormones have direct impact on placental function by modulating placental nutrient transport. Nutrient delivery to the fetus is regulated by a complex interaction between insulin signaling, cytokine profile and insulin responsiveness, which is modulated by adiponectin and IL-1β. In addition, obese pregnant women are at risk for hypertension and preeclampsia with reduced placental vascularity and blood flow, which would restrict placental nutrient delivery to the developing fetus. These sometimes opposing signals regulating placental function may contribute to the diversity of short and long-term outcomes observed in pregnant obese women. This review focuses on the changes in adipokines and obesity-related metabolic hormones, how these factors influence placental function and fetal development to contribute to long-term metabolic and behavioral consequences of children born to obese mothers. PMID:27864335

  17. Drug-nutrient interactions: a case and clinical guide.

    PubMed

    Plotnikoff, Gregory A

    2011-10-01

    Advances in pharmacokinetics and pharmacodynamics require new competencies related to pharmaceutical prescribing. First, both physicians and pharmacists need to recognize the potential negative impact of nutrients and dietary supplements on the absorption, metabolism, and utilization of prescription drugs. Second, physicians, even more than pharmacists, need to recognize the potential negative effects of pharmaceuticals on the absorption, metabolism, and utilization of nutrients. This article discusses common drug-nutrient interactions and presents a case that illustrates how unrecognized nutrient disruption may negatively affect a patient's health and potentially result in unnecessary prescribing of medications. In presenting the case, we also provide a conceptual framework for assessing and treating this patient and a summary of current knowledge regarding drug-nutrient interactions.

  18. Plant Nutrition 2: Macronutrients (N, P, K, S, Mg, and Ca)

    PubMed Central

    2014-01-01

    Summary In the second of three lessons spanning the topic of Plant Nutrition, we examine how macronutrients affect plant growth. Specifically, we look at (1) the availability of nutrients in the soil along with the effects of soil microbes and physical properties on their availability; (2) nutrient uptake from the external environment, across plasma membranes and into plant cells; (3) in some cases, the assimilation of the nutrient into organic molecules; (4) the distribution and redistribution of nutrients throughout the plant; and (5) regulation of these processes. In parallel, we examine the genetic basis of a plant's nutrient use efficiency (NUE) and evaluate strategies by which to replenish nutrients that growing plants extract from soil.

  19. Cumulative Effects of Nutrient Enrichment and Elevated Temperature Compromise the Early Life History Stages of the Coral Acropora tenuis

    PubMed Central

    Noonan, Sam H. C.; Willis, Bette L.; Fabricius, Katharina E.; Negri, Andrew P.

    2016-01-01

    Inshore coral reefs are experiencing the combined pressures of excess nutrient availability associated with coastal activities and warming seawater temperatures. Both pressures are known to have detrimental effects on the early life history stages of hard corals, but studies of their combined effects on early demographic stages are lacking. We conducted a series of experiments to test the combined effects of nutrient enrichment (three levels) and elevated seawater temperature (up to five levels) on early life history stages of the inshore coral Acropora tenuis, a common species in the Indo-Pacific and Red Sea. Gamete fertilization, larval survivorship and larval settlement were all significantly reduced as temperature increased, but only fertilization was further affected by simultaneous nutrient enrichment. Combined high temperatures and nutrient enrichment affected fertilization in an additive manner, whereas embryo abnormalities increased synergistically. Higher than normal temperatures (32°C) increased coral juvenile growth rates 1.6-fold, but mortality also increased by 50%. The co-occurrence of nutrient enrichment with high temperatures reduced juvenile mortality to 36%, ameliorating temperature stress (antagonistic interaction). Overall, the types of effect (additive vs synergistic or antagonistic) and their magnitude varied among life stages. Gamete and embryo stages were more affected by temperature stress and, in some cases, also by nutrient enrichment than juveniles. The data suggest that coastal runoff events might exacerbate the impacts of warming temperatures on fertilization if these events co-occur during corals spawning. The cumulative impacts of simultaneous exposure to nutrient enrichment and elevated temperatures over all early life history stages increases the likelihood for failure of larval supply and recruitment for this coral species. Our results suggest that improving the water quality of river discharges into coastal areas might help to enhance the thermal tolerances of early life history stages in this common coral species. PMID:27575699

  20. Hydrological Controls on Nutrient Concentrations and Fluxes in Agricultural Catchments

    NASA Astrophysics Data System (ADS)

    Petry, J.; Soulsby, C.

    2002-12-01

    This investigation into diffuse agricultural pollution and the hydrological controls that exert a strong influence on both nutrient concentrations and fluxes, was conducted in an intensively farmed lowland catchment in north-east Scotland. The study focuses on spatial and seasonal variations in nutrient concentrations and fluxes at the catchment scale, over a 15-month period. The water quality of the 14.5 km2 Newmills Burn catchment has relatively high nutrient levels with mean concentrations of NO3-N and NH3-N at 6.09 mg/l and 0.28 mg/l respectively. Average PO4-P concentrations are 0.06 mg/l. Over short timescales nutrient concentrations and fluxes are greatest during storm events when PO4-P and NH3-N are mobilised by overland flow in riparian areas, where soils have been compacted by livestock or machinery. Delivery of deeper soil water in subsurface storm flow, facilitated by agricultural under-drainage, produces a marked increase in NO3-N (6.9 mg/l) concentrations on the hydrograph recession limb. A more detailed insight into the catchment response to storm events, and in particular the response of the hydrological pathways which provide the main sources of runoff during storm events, was gained by sampling stream water at 2-hourly intervals during 5 events. End Member Mixing Analysis (EMMA) was carried out using event specific end-member chemistries to differentiate three catchment-scale hydrological pathways (overland flow, subsurface storm flow, groundwater flow) on the basis of observed Si and NO3-N concentrations in sampled source waters. Results show that overland flow generally dominates the storm peak and provides the main flow path by which P is transferred to stream channels during storm events, whilst subsurface storm flows usually dominate the storm hydrograph volumetrically and route NO3-rich soil water to the stream. The study shows that altering hydrological pathways in a catchment can have implications for nutrient management. Whilst buffer strips can reduce the delivery of NH3-N and PO4-P by overland flow to stream channels during storm events, the management of N-rich storm runoff as NO3 via sub-surface drains would require significant interference with the drainage network. This could have a negative impact on agricultural production in the catchment.

  1. Nutrients stimulate leaf breakdown rates and detritivore biomass: Bottom-up effects via heterotrophic pathways

    USGS Publications Warehouse

    Greenwood, J.L.; Rosemond, A.D.; Wallace, J.B.; Cross, W.F.; Weyers, H.S.

    2007-01-01

    Most nutrient enrichment studies in aquatic systems have focused on autotrophic food webs in systems where primary producers dominate the resource base. We tested the heterotrophic response to long-term nutrient enrichment in a forested, headwater stream. Our study design consisted of 2 years of pretreatment data in a reference and treatment stream and 2 years of continuous nitrogen (N) + phosphorus addition to the treatment stream. Studies were conducted with two leaf species that differed in initial C:N, Rhododendron maximum (rhododendron) and Acer rubrum (red maple). We determined the effects of nutrient addition on detrital resources (leaf breakdown rates, litter C:N and microbial activity) and tested whether nutrient enrichment affected macroinvertebrate consumers via increased biomass. Leaf breakdown rates were ca. 1.5 and 3?? faster during the first and second years of enrichment, respectively, in the treatment stream for both leaf types. Microbial respiration rates of both leaf types were 3?? higher with enrichment, and macroinvertebrate biomass associated with leaves increased ca. 2-3?? with enrichment. The mass of N in macroinvertebrate biomass relative to leaves tended to increase with enrichment up to 6?? for red maple and up to 44?? for rhododendron leaves. Lower quality (higher C:N) rhododendron leaves exhibited greater changes in leaf nutrient content and macroinvertebrate response to nutrient enrichment than red maple leaves, suggesting a unique response by different leaf species to nutrient enrichment. Nutrient concentrations used in this study were moderate and equivalent to those in streams draining watersheds with altered land use. Thus, our results suggest that similarly moderate levels of enrichment may affect detrital resource quality and subsequently lead to altered energy and nutrient flow in detrital food webs. ?? 2006 Springer-Verlag.

  2. LHRH-Targeted Drug Delivery Systems for Cancer Therapy.

    PubMed

    Li, Xiaoning; Taratula, Oleh; Taratula, Olena; Schumann, Canan; Minko, Tamara

    2017-01-01

    Targeted delivery of therapeutic and diagnostic agents to cancer sites has significant potential to improve the therapeutic outcome of treatment while minimizing severe side effects. It is widely accepted that decoration of the drug delivery systems with targeting ligands that bind specifically to the receptors on the cancer cells is a promising strategy that may substantially enhance accumulation of anticancer agents in the tumors. Due to the transformed cellular nature, cancer cells exhibit a variety of overexpressed cell surface receptors for peptides, hormones, and essential nutrients, providing a significant number of target candidates for selective drug delivery. Among others, luteinizing hormonereleasing hormone (LHRH) receptors are overexpressed in the majority of cancers, while their expression in healthy tissues, apart from pituitary cells, is limited. The recent studies indicate that LHRH peptides can be employed to efficiently guide anticancer and imaging agents directly to cancerous cells, thereby increasing the amount of these substances in tumor tissue and preventing normal cells from unnecessary exposure. This manuscript provides an overview of the targeted drug delivery platforms that take advantage of the LHRH receptors overexpression by cancer cells.

  3. New studies initiated by the U.S. Geological Survey - Effects of nutrient enrichment on stream ecosystems

    USGS Publications Warehouse

    Munn, Mark D.; Hamilton, Pixie A.

    2003-01-01

    In 2001, the U.S. Geological Survey’s National Water-Quality Assessment (NAWQA) Program began an intensive study of nutrient enrichment elevated concentrations of nitrogen and phosphorus in streams in five agricultural basins across the Nation (see map, p. 2). This study is providing nationally consistent and comparable data and analyses of nutrient conditions, including how these conditions vary as a result of natural and human-related factors, and how nutrient conditions affect algae and other biological communities. This information will benefit stakeholders, including the U.S. Environmental Protection Agency (USEPA) and its partners, who are developing nutrient criteria to protect the aquatic health of streams in different geographic regions.Nutrient enrichment is one of five national priority topics addressed by NAWQA in its second decade of studies, which began in 2001. During its first round of assessments in 51 major river basins (referred to as “Study Units”), NAWQA scientists collected data on water chemistry, stream hydrology and habitat, and biological communities; currently, NAWQA is revisiting selected basins and focusing on (1) trends, (2) factors that affect water quality and aquatic health, and (3) national priority water issues related to, for example, the development of nutrient criteria, source-water protection strategies, and stream restoration plans.The nutrient enrichment study has four major objectives that address nutrient conditions, dissolved oxygen, aquatic communities, and geographic and landscape features in agricultural basins (see inset). The focus on agricultural streams is a starting point. As the study progresses, streams draining other land uses, such as those in residential and urban areas, will likely be added.

  4. Plant litter decomposition and nutrient release in peatlands

    NASA Astrophysics Data System (ADS)

    Bragazza, Luca; Buttler, Alexandre; Siegenthaler, Andy; Mitchell, Edward A. D.

    Decomposition of plant litter is a crucial process in controlling the carbon balance of peatlands. Indeed, as long as the rate of litter decomposition remains lower than the rate of above- and belowground litter production, a net accumulation of peat and, thus, carbon will take place. In addition, decomposition controls the release of important nutrients such as nitrogen, phosphorus, and potassium, the availability of which affects the structure and the functioning of plant communities. This chapter describes the role of the main drivers in affecting mass loss and nutrient release from recently deposited plant litter. In particular, the rate of mass loss of Sphagnum litter and vascular plant litter is reviewed in relation to regional climatic conditions, aerobic/anaerobic conditions, and litter chemistry. The rate of nutrient release is discussed in relation to the rate of mass loss and associated litter chemistry by means of a specific case study.

  5. Escherichia coli growth under modeled reduced gravity

    NASA Technical Reports Server (NTRS)

    Baker, Paul W.; Meyer, Michelle L.; Leff, Laura G.

    2004-01-01

    Bacteria exhibit varying responses to modeled reduced gravity that can be simulated by clino-rotation. When Escherichia coli was subjected to different rotation speeds during clino-rotation, significant differences between modeled reduced gravity and normal gravity controls were observed only at higher speeds (30-50 rpm). There was no apparent affect of removing samples on the results obtained. When E. coli was grown in minimal medium (at 40 rpm), cell size was not affected by modeled reduced gravity and there were few differences in cell numbers. However, in higher nutrient conditions (i.e., dilute nutrient broth), total cell numbers were higher and cells were smaller under reduced gravity compared to normal gravity controls. Overall, the responses to modeled reduced gravity varied with nutrient conditions; larger surface to volume ratios may help compensate for the zone of nutrient depletion around the cells under modeled reduced gravity.

  6. Thermal acclimation and nutritional history affect the oxidation of different classes of exogenous nutrients in Siberian hamsters, Phodopus sungorus.

    PubMed

    McCue, Marshall D; Voigt, Christian C; Jefimow, Małgorzata; Wojciechowski, Michał S

    2014-11-01

    During acclimatization to winter, changes in morphology and physiology combined with changes in diet may affect how animals use the nutrients they ingest. To study (a) how thermal acclimation and (b) nutritional history affect the rates at which Siberian hamsters (Phodopus sungorus) oxidize different classes of dietary nutrients, we conducted two trials in which we fed hamsters one of three (13) C-labeled compounds, that is, glucose, leucine, or palmitic acid. We predicted that under acute cold stress (3 hr at 2°C) hamsters previously acclimated to cold temperatures (10°C) for 3 weeks would have higher resting metabolic rate (RMR) and would oxidize a greater proportion of dietary fatty acids than animals acclimated to 21°C. We also investigated how chronic nutritional stress affects how hamsters use dietary nutrients. To examine this, hamsters were fed four different diets (control, low protein, low lipid, and low-glycemic index) for 2 weeks. During cold challenges, hamsters previously acclimated to cold exhibited higher thermal conductance and RMR, and also oxidized more exogenous palmitic acid during the postprandial phase than animals acclimated to 21°C. In the nutritional stress trial, hamsters fed the low protein diet oxidized more exogenous glucose, but not more exogenous palmitic acid than the control group. The use of (13) C-labeled metabolic tracers combined with breath testing demonstrated that both thermal and nutritional history results in significant changes in the extent to which animals oxidize dietary nutrients during the postprandial period. © 2014 Wiley Periodicals, Inc.

  7. Diffusion Performance of Fertilizer Nutrient through Polymer Latex Film.

    PubMed

    An, Di; Yang, Ling; Liu, Boyang; Wang, Ting-Jie; Kan, Chengyou

    2017-12-20

    Matching the nutrient release rate of coated fertilizer with the nutrient uptake rate of the crop is the best way to increase the utilization efficiency of nutrients and reduce environmental pollution from the fertilizer. The diffusion property and mechanism of nutrients through the film are the theoretical basis for the product pattern design of coated fertilizers. For the coated fertilizer with a single-component nutrient, an extended solution-diffusion model was used to describe the difference of nutrient release rate, and the release rate is proportional to the permeation coefficient and the solubility of the nutrient. For the double- and triple-component fertilizer of N-K, N-P, and N-P-K, because of the interaction among nutrient molecules and ions, the release rates of different nutrients were significantly affected by the components in the composite fertilizer. Coating the single-component fertilizer (i.e., nitrogen fertilizer, phosphate fertilizer, and potash fertilizer) first and subsequently bulk blending is expected to be a promising way to adjust flexibly the nutrient release rate to meet the nutrient uptake rate of the crop.

  8. Influence of storm characteristics on soil erosion and storm runoff

    Treesearch

    Johnny M. III Grace

    2008-01-01

    Unpaved forest roads can be major sources of sediment from forested watersheds. Storm runoff from forest roads are a concern due to their potential delivery of sediments and nutrients to stream systems resulting in degraded water quality. The volume and sediment concentrations of stormwater runoff emanating from forest roads can be greatly influenced by storm...

  9. Ak-Chin Indian Community Biomass Feasiiblity Study

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mark A. Moser, RCM Digesters, Inc.; Mark Randall, Daystar Consulting, LLC; Leonard S. Gold, Ak-Chin Energy Services & Utility Strategies Consulting Group

    2005-12-31

    Study of the conversion of chicken litter to biogas for the production of energy. There was an additional requirement that after extracting the energy from the chicken litter the nutrient value of the raw chicken litter had to be returned to the Ak-Chin Farms for use as fertilizer in a form and delivery method acceptable to the Farm.

  10. Characterization of the proteome of cytoplasmic lipid droplets in mouse enterocytes after a dietary fat challenge

    USDA-ARS?s Scientific Manuscript database

    Dietary fat absorption by the small intestine is a multistep process that regulates the uptake and delivery of essential nutrients and energy. One step of this process is the temporary storage of dietary fat in cytoplasmic lipid droplets (CLDs). The storage and mobilization of dietary fat is thought...

  11. Double strand RNA oral delivery methods to induce RNA interference in phloem and plant-sap-feeding insects

    USDA-ARS?s Scientific Manuscript database

    Phloem and plant sap feeding insect pests invade the integrity of crops and fruits to retrieve nutrients in the process damaging food productivity. Hemipteran insects account for a number of economically substantial pests of plants that cause damage to crops by feeding on phloem sap. Halyomorpha hal...

  12. Plant–herbivore–decomposer stoichiometric mismatches and nutrient cycling in ecosystems

    PubMed Central

    Cherif, Mehdi; Loreau, Michel

    2013-01-01

    Plant stoichiometry is thought to have a major influence on how herbivores affect nutrient availability in ecosystems. Most conceptual models predict that plants with high nutrient contents increase nutrient excretion by herbivores, in turn raising nutrient availability. To test this hypothesis, we built a stoichiometrically explicit model that includes a simple but thorough description of the processes of herbivory and decomposition. Our results challenge traditional views of herbivore impacts on nutrient availability in many ways. They show that the relationship between plant nutrient content and the impact of herbivores predicted by conceptual models holds only at high plant nutrient contents. At low plant nutrient contents, the impact of herbivores is mediated by the mineralization/immobilization of nutrients by decomposers and by the type of resource limiting the growth of decomposers. Both parameters are functions of the mismatch between plant and decomposer stoichiometries. Our work provides new predictions about the impacts of herbivores on ecosystem fertility that depend on critical interactions between plant, herbivore and decomposer stoichiometries in ecosystems. PMID:23303537

  13. The effects of CO2 and nutrient fertilisation on the growth and temperature response of the mangrove Avicennia germinans.

    PubMed

    Reef, Ruth; Slot, Martijn; Motro, Uzi; Motro, Michal; Motro, Yoav; Adame, Maria F; Garcia, Milton; Aranda, Jorge; Lovelock, Catherine E; Winter, Klaus

    2016-08-01

    In order to understand plant responses to both the widespread phenomenon of increased nutrient inputs to coastal zones and the concurrent rise in atmospheric CO2 concentrations, CO2-nutrient interactions need to be considered. In addition to its potential stimulating effect on photosynthesis and growth, elevated CO2 affects the temperature response of photosynthesis. The scarcity of experiments testing how elevated CO2 affects the temperature response of tropical trees hinders our ability to model future primary productivity. In a glasshouse study, we examined the effects of elevated CO2 (800 ppm) and nutrient availability on seedlings of the widespread mangrove Avicennia germinans. We assessed photosynthetic performance, the temperature response of photosynthesis, seedling growth and biomass allocation. We found large synergistic gains in both growth (42 %) and photosynthesis (115 %) when seedlings grown under elevated CO2 were supplied with elevated nutrient concentrations relative to their ambient growing conditions. Growth was significantly enhanced under elevated CO2 only under high-nutrient conditions, mainly in above-ground tissues. Under low-nutrient conditions and elevated CO2, root volume was more than double that of seedlings grown under ambient CO2 levels. Elevated CO2 significantly increased the temperature optimum for photosynthesis by ca. 4 °C. Rising CO2 concentrations are likely to have a significant positive effect on the growth rate of A. germinans over the next century, especially in areas where nutrient availability is high.

  14. Interacting Physical and Biological Processes Affecting Nutrient Transport Through Human Dominated Landscapes

    NASA Astrophysics Data System (ADS)

    Finlay, J. C.

    2015-12-01

    Human activities increasingly dominate biogeochemical cycles of limiting nutrients on Earth. Urban and agricultural landscapes represent the largest sources of excess nutrients that drive water quality degradation. The physical structure of both urban and agricultural watersheds has been extensively modified, and these changes have large impacts on water and nutrient transport. Despite strong physical controls over nutrient transport in human dominated landscapes, biological processes play important roles in determining the fates of both nitrogen and phosphorus. This talk uses examples from research in urban and agricultural watersheds in the Midwestern USA to illustrate interactions of physical and biological controls over nutrient cycles that have shifted nitrogen (N) and phosphorus (P) sources and cycling in unexpected ways in response to management changes. In urban watersheds, efforts to improve water quality have been hindered by legacy sources of phosphorus added to storm water through transport to drainage systems by vegetation. Similarly, reductions in field erosion in agricultural watersheds have not led to major reductions in phosphorus transport, because of continued release of biological sources of P. Where management of phosphorus has been most effective in reducing eutrophication of lakes, decreases in N removal processes have led to long term increases in N concentration and transport. Together, these examples show important roles for biological processes affecting nutrient movement in highly modified landscapes. Consideration of the downstream physical and biological responses of management changes are thus critical toward identification of actions that will most effectively reduce excess nutrients watersheds and coastal zones.

  15. Impact of delivery mode on the colostrum microbiota composition.

    PubMed

    Toscano, Marco; De Grandi, Roberta; Peroni, Diego Giampietro; Grossi, Enzo; Facchin, Valentina; Comberiati, Pasquale; Drago, Lorenzo

    2017-09-25

    Breast milk is a rich nutrient with a temporally dynamic nature. In particular, numerous alterations in the nutritional, immunological and microbiological content occur during the transition from colostrum to mature milk. The objective of our study was to evaluate the potential impact of delivery mode on the microbiota of colostrum, at both the quantitative and qualitative levels (bacterial abundance and microbiota network). Twenty-nine Italian mothers (15 vaginal deliveries vs 14 Cesarean sections) were enrolled in the study. The microbiota of colostrum samples was analyzed by next generation sequencing (Ion Torrent Personal Genome Machine). The colostrum microbiota network associated with Cesarean section and vaginal delivery was evaluated by means of the Auto Contractive Map (AutoCM), a mathematical methodology based on Artificial Neural Network (ANN) architecture. Numerous differences between Cesarean section and vaginal delivery colostrum were observed. Vaginal delivery colostrum had a significant lower abundance of Pseudomonas spp., Staphylococcus spp. and Prevotella spp. when compared to Cesarean section colostrum samples. Furthermore, the mode of delivery had a strong influence on the microbiota network, as Cesarean section colostrum showed a higher number of bacterial hubs if compared to vaginal delivery, sharing only 5 hubs. Interestingly, the colostrum of mothers who had a Cesarean section was richer in environmental bacteria than mothers who underwent vaginal delivery. Finally, both Cesarean section and vaginal delivery colostrum contained a greater number of anaerobic bacteria genera. The mode of delivery had a large impact on the microbiota composition of colostrum. Further studies are needed to better define the meaning of the differences we observed between Cesarean section and vaginal delivery colostrum microbiota.

  16. Chitosan-Based Multifunctional Platforms for Local Delivery of Therapeutics

    PubMed Central

    Hong, Seong-Chul; Yoo, Seung-Yup; Kim, Hyeongmin; Lee, Jaehwi

    2017-01-01

    Chitosan has been widely used as a key biomaterial for the development of drug delivery systems intended to be administered via oral and parenteral routes. In particular, chitosan-based microparticles are the most frequently employed delivery system, along with specialized systems such as hydrogels, nanoparticles and thin films. Based on the progress made in chitosan-based drug delivery systems, the usefulness of chitosan has further expanded to anti-cancer chemoembolization, tissue engineering, and stem cell research. For instance, chitosan has been used to develop embolic materials designed to efficiently occlude the blood vessels by which the oxygen and nutrients are supplied. Indeed, it has been reported to be a promising embolic material. For better anti-cancer effect, embolic materials that can locally release anti-cancer drugs were proposed. In addition, a complex of radioactive materials and chitosan to be locally injected into the liver has been investigated as an efficient therapeutic tool for hepatocellular carcinoma. In line with this, a number of attempts have been explored to use chitosan-based carriers for the delivery of various agents, especially to the site of interest. Thus, in this work, studies where chitosan-based drug delivery systems have successfully been used for local delivery will be presented along with future perspectives. PMID:28257059

  17. Continuous hydroponic wheat production using a recirculating system

    NASA Technical Reports Server (NTRS)

    Mackowiak, C. L.; Owens, L. P.; Hinkle, C. R.; Prince, R. P.

    1989-01-01

    Continuous crop production, where plants of various ages are growing simultaneously in a single recirculating nutrient solution, is a possible alternative to batch production in a Controlled Ecological Life Support System. A study was conducted at John F. Kennedy Space Center where 8 trays (0.24 sq m per tray) of Triticum aestivum L. Yecora Rojo were grown simultaneously in a growth chamber at 23 C, 65 percent relative humidity, 1000 ppm CO2, continuous light, with a continuous flow, thin film nutrient delivery system. The same modified Hoagland nutrient solution was recirculated through the plant trays from an 80 L reservoir throughout the study. It was maintained by periodic addition of water and nutrients based on chemical analyses of the solution. The study was conducted for 216 days, during which 24 trays of wheat were consecutively planted (one every 9 days), 16 of which were grown to maturity and harvested. The remaining 8 trays were harvested on day 216. Grain yields averaged 520 g m(exp -2), and had an average edible biomass of 32 percent. Consecutive yields were unaffected by nutrient solution age. It was concluded that continual wheat production will work in this system over an extended period of time. Certain micronutrient deficiencies and toxicities posed problems and must be addressed in future continuous production systems.

  18. Challenges with effective nutrient supplementation for amphibians: A review of cricket studies.

    PubMed

    Livingston, Shannon; Lavin, Shana R; Sullivan, Kathleen; Attard, Lydia; Valdes, Eduardo V

    2014-01-01

    Over the last 25 years, numerous studies have investigated the impact of insect supplementation on insect nutrient content. In light of recent nutrition related challenges with regards to zoo amphibians fed an insect based diet, this review attempts to comprehensively compile both anecdotal and published data in the context of practical application on this topic. Insects, primarily crickets, used for amphibian diets historically demonstrate low concentrations of key nutrients including calcium and vitamin A. Commonly used practices for supplementation involving powder dusting or gut loading have been shown to improve delivery of calcium and vitamin A, though often not reaching desired nutrient concentrations. The large variety of factors influencing insect nutrient content are difficult to control, making study design, and results often inconsistent. Formulation and availability of more effective gut loading diets, combined with a standardized protocol for insect husbandry and dietary management may be the most effective way to supplement insects for use in amphibian feeding programs. Ideally, the nutritional improvement of feeder insects would begin at the breeder level; however, until this becomes a viable choice, we confirm that supplementation of crickets through both gut-loading and dusting appear necessary to support the nutritional health of amphibians and other insectivores in managed collections. © 2014 Wiley Periodicals, Inc.

  19. Assessing and managing drug-nutrient interactions.

    PubMed

    Anderson, Karl E; Greenblatt, David J

    2002-01-01

    Drug-nutrient interactions can occur through many mechanisms. The amount of protein in the diet and the presence of micronutrients, such as polycyclic aromatic hydrocarbons and indoles, can affect drug metabolism. Although furanocoumarins in grapefruit juice can interact with certain oral medications, noninteracting medications generally can be substituted. Pharmacists need to provide patients with accurate information about drug-nutrient interactions and help to clarify common misconceptions about these effects.

  20. Nutrient Distribution and Tree Development Through Age 8 of Four Oaks Planted at Five Spacings in a Minor Stream Bottom

    Treesearch

    Harvey E. Kennedy; Bryce E. Schlaegel; Roger M. Krinard

    1986-01-01

    Eight hardwood species were planted at five spacings in a minor stream bottom in southeast Arkansas. Because of inherent differences in nutrient requirements and tree development, only four oak species are covered in this paper. Spacing generally did not affect nutrient concentrations, but differences did exist among species. Leaves constituted only 10-15 percent of...

  1. Management and the conservation of freshwater ecosystems

    USGS Publications Warehouse

    Wipfli, Mark S.; Richardson, John S.

    2015-01-01

    Riparian and freshwater ecosystems are typically tightly coupled, especially in their natural states, and the linkages that couple them frequently exert strong influence on their associated invertebrate and fish fauna (e.g. Gregory et al., 1991; Naiman et al., 2010). Riparian habitats, and the condition of these habitats, further plays a key role in the ecology of these fresh waters, influencing critical processes such as water, nutrient and sediment delivery and dynamics; prey resources for fish and other consumers, and other organic materials exchanged between aquatic and terrestrial habitats (Nakano et al., 1999; Naiman et al., 2010); light and water temperature dynamics that in turn affect food web processes and fish metabolism and growth; aquatic physical habitat (wood); and terrestrial consumers that prey upon fishes (Bisson & Bilby, 1998; Naiman et al., 2010; Wipfli & Baxter, 2010). These processes in turn directly or indirectly influence fishes in freshwater systems (Wang et al., 2001; Pusey & Arthington, 2003; Allan, 2004; Richardson et al., 2010a).

  2. Red light and carbon dioxide differentially affect growth, lipid production, and quality in the microalga, Ettlia oleoabundans.

    PubMed

    Yang, Ying; Weathers, Pamela

    2015-01-01

    Ettlia oleoabundans, a freshwater unicellular green microalga, was grown under different light qualities ± carbon dioxide-enriched air to determine the combined effects on growth and lipid production of this oleaginous species. Keeping total light intensity constant, when a portion of the cool white was replaced by red, volumetric lipid yield increased 2.8-fold mainly due to the greater yield of oleic acid, a desirable biodiesel precursor. Only 30 min of red light treatment was sufficient to increase lipid yield and quality to the same level as cultures provided red light for >14 days, indicating the potential role of red light in stimulating lipid production of this species. Carbon dioxide enrichment via air sparging enhanced exponential growth, carbon conversion efficiency, and nutrient consumption. Together, these results showed that light quality plays an important role in microalgal lipid production. Adjustment in light quality and gas delivery efficiency with carbon dioxide enrichment improved lipid yield and quality in this and possibly other oleaginous algal species.

  3. Nutrient enrichment is associated with altered nectar and pollen chemical composition in Succisa pratensis Moench and increased larval mortality of its pollinator Bombus terrestris L.

    PubMed

    Ceulemans, Tobias; Hulsmans, Eva; Vanden Ende, Wim; Honnay, Olivier

    2017-01-01

    Pollinators are declining worldwide and possible underlying causes include disease, invasive pest species and large scale land use changes resulting in habitat loss and degradation. One particular cause of habitat degradation is the increased inflow of nutrients due to anthropogenic combustion processes and large scale application of agricultural fertilizers. This nutrient pollution has been shown to affect pollinators through the loss of nectar and pollen-providing plant species. However, it may also affect pollinators through altering the nectar and pollen chemical composition of plant species, hence influencing pollinator food quality. Here, we experimentally investigated the effect of nutrient enrichment on amino acid and sugar composition of nectar and pollen in the grassland plant Sucissa pratensis, and the subsequent colony size and larval mortality of the pollinating bumblebee Bombus terrestris. We found less of the essential amino acids glycine and arginine in the pollen of fertilized plants, and more arginine, ornithine and threonine in the pollen of control plants. Nectar glucose and pollen fructose levels were lower in fertilized plants as compared to control plants. Furthermore, bumblebee colonies visiting fertilized plants showed more dead larvae than colonies visiting control plants. Our results suggest that the fitness of bumblebees can be negatively affected by changes in their food quality following nutrient pollution. If similar patterns hold for other plant and pollinator species, this may have far reaching implications for the maintenance of pollination ecosystem services, as nutrient pollution continues to rise worldwide.

  4. Nutrient enrichment is associated with altered nectar and pollen chemical composition in Succisa pratensis Moench and increased larval mortality of its pollinator Bombus terrestris L.

    PubMed Central

    Vanden Ende, Wim; Honnay, Olivier

    2017-01-01

    Pollinators are declining worldwide and possible underlying causes include disease, invasive pest species and large scale land use changes resulting in habitat loss and degradation. One particular cause of habitat degradation is the increased inflow of nutrients due to anthropogenic combustion processes and large scale application of agricultural fertilizers. This nutrient pollution has been shown to affect pollinators through the loss of nectar and pollen-providing plant species. However, it may also affect pollinators through altering the nectar and pollen chemical composition of plant species, hence influencing pollinator food quality. Here, we experimentally investigated the effect of nutrient enrichment on amino acid and sugar composition of nectar and pollen in the grassland plant Sucissa pratensis, and the subsequent colony size and larval mortality of the pollinating bumblebee Bombus terrestris. We found less of the essential amino acids glycine and arginine in the pollen of fertilized plants, and more arginine, ornithine and threonine in the pollen of control plants. Nectar glucose and pollen fructose levels were lower in fertilized plants as compared to control plants. Furthermore, bumblebee colonies visiting fertilized plants showed more dead larvae than colonies visiting control plants. Our results suggest that the fitness of bumblebees can be negatively affected by changes in their food quality following nutrient pollution. If similar patterns hold for other plant and pollinator species, this may have far reaching implications for the maintenance of pollination ecosystem services, as nutrient pollution continues to rise worldwide. PMID:28406910

  5. Taste and mouthfeel assessment of porous and non-porous silicon microparticles

    NASA Astrophysics Data System (ADS)

    Shabir, Qurrat; Skaria, Cyrus; Brien, Heather O.; Loni, Armando; Barnett, Christian; Canham, Leigh

    2012-07-01

    Unlike the trace minerals iron, copper and zinc, the semiconductor silicon has not had its organoleptic properties assessed. Nanostructured silicon provides the nutrient orthosilicic acid through hydrolysis in the gastrointestinal tract and is a candidate for oral silicon supplements. Mesoporous silicon, a nanostructured material, is being assessed for both oral drug and nutrient delivery. Here we use taste panels to determine the taste threshold and taste descriptors of both solid and mesoporous silicon in water and chewing gum base. Comparisons are made with a metal salt (copper sulphate) and porous silica. We believe such data will provide useful benchmarks for likely consumer acceptability of silicon supplemented foodstuffs and beverages.

  6. Seabirds supply nitrogen to reef-building corals on remote Pacific islets.

    PubMed

    Lorrain, Anne; Houlbrèque, Fanny; Benzoni, Francesca; Barjon, Lucie; Tremblay-Boyer, Laura; Menkes, Christophe; Gillikin, David P; Payri, Claude; Jourdan, Hervé; Boussarie, Germain; Verheyden, Anouk; Vidal, Eric

    2017-06-16

    Seabirds concentrate nutrients from large marine areas on their nesting islands playing an important ecological role in nutrient transfer between marine and terrestrial ecosystems. Here we investigate the role of guano on corals reefs across scales by analyzing the stable nitrogen isotopic (δ 15 N) values of the scleractinian coral Pocillopora damicornis on fringing reefs around two Pacific remote islets with large seabird colonies. Marine stations closest to the seabird colonies had higher nitrate + nitrite concentrations compared to more distant stations. Coral and zooxanthellae δ 15 N values were also higher at these sites, suggesting that guano-derived nitrogen is assimilated into corals and contributes to their nitrogen requirements. The spatial extent of guano influence was however restricted to a local scale. Our results demonstrate that seabird-derived nutrients not only spread across the terrestrial ecosystem, but also affect components of the adjacent marine ecosystem. Further studies are now needed to assess if this nutrient input has a positive or negative effect for corals. Such studies on remote islets also open fresh perspectives to understand how nutrients affect coral reefs isolated from other anthropogenic stressors.

  7. Nanotechnology and its applications in the food sector.

    PubMed

    Sozer, Nesli; Kokini, Jozef L

    2009-02-01

    Nanoscience and nanotechnology are new frontiers of this century. Their applications to the agriculture and food sector are relatively recent compared with their use in drug delivery and pharmaceuticals. Smart delivery of nutrients, bioseparation of proteins, rapid sampling of biological and chemical contaminants and nanoencapsulation of nutraceuticals are some of the emerging topics of nanotechnology for food and agriculture. Advances in technologies, such as DNA microarrays, microelectromechanical systems and microfluidics, will enable the realization of the potential of nanotechnology for food applications. In this review, we intended to summarize the applications of nanotechnology relevant to food and nutraceuticals together with identifying the outstanding challenges.

  8. System analysis of metabolism and the transcriptome in Arabidopsis thaliana roots reveals differential co-regulation upon iron, sulfur and potassium deficiency.

    PubMed

    Forieri, Ilaria; Sticht, Carsten; Reichelt, Michael; Gretz, Norbert; Hawkesford, Malcolm J; Malagoli, Mario; Wirtz, Markus; Hell, Ruediger

    2017-01-01

    Deprivation of mineral nutrients causes significant retardation of plant growth. This retardation is associated with nutrient-specific and general stress-induced transcriptional responses. In this study, we adjusted the external supply of iron, potassium and sulfur to cause the same retardation of shoot growth. Nevertheless, limitation by individual nutrients resulted in specific morphological adaptations and distinct shifts within the root metabolite fingerprint. The metabolic shifts affected key metabolites of primary metabolism and the stress-related phytohormones, jasmonic, salicylic and abscisic acid. These phytohormone signatures contributed to specific nutrient deficiency-induced transcriptional regulation. Limitation by the micronutrient iron caused the strongest regulation and affected 18% of the root transcriptome. Only 130 genes were regulated by all nutrients. Specific co-regulation between the iron and sulfur metabolic routes upon iron or sulfur deficiency was observed. Interestingly, iron deficiency caused regulation of a different set of genes of the sulfur assimilation pathway compared with sulfur deficiency itself, which demonstrates the presence of specific signal-transduction systems for the cross-regulation of the pathways. Combined iron and sulfur starvation experiments demonstrated that a requirement for a specific nutrient can overrule this cross-regulation. The comparative metabolomics and transcriptomics approach used dissected general stress from nutrient-specific regulation in roots of Arabidopsis. © 2016 John Wiley & Sons Ltd.

  9. Streamflow and Nutrient Fluxes of the Mississippi-Atchafalaya River Basin and Subbasins for the Period of Record Through 2005

    USGS Publications Warehouse

    Aulenbach, Brent T.; Buxton, Herbert T.; Battaglin, William A.; Coupe, Richard H.

    2007-01-01

    U.S. Geological Survey has monitored streamflow and water quality systematically in the Mississippi-Atchafalaya River Basin (MARB) for more than five decades. This report provides streamflow and estimates of nutrient delivery (flux) to the Gulf of Mexico from both the Atchafalaya River and the main stem of the Mississippi River. This report provides streamflow and nutrient flux estimates for nine major subbasins of the Mississippi River. This report also provides streamflow and flux estimates for 21 selected subbasins of various sizes, hydrology, land use, and geographic location within the Basin. The information is provided at each station for the period for which sufficient water-quality data are available to make statistically based flux estimates (starting as early as water year1 1960 and going through water year 2005). Nutrient fluxes are estimated using the adjusted maximum likelihood estimate, a type of regression-model method; nutrient fluxes to the Gulf of Mexico also are estimated using the composite method. Regression models were calibrated using a 5-year moving calibration period; the model was used to estimate the last year of the calibration period. Nutrient flux estimates are provided for six water-quality constituents: dissolved nitrite plus nitrate, total organic nitrogen plus ammonia nitrogen (total Kjeldahl nitrogen), dissolved ammonia, total phosphorous, dissolved orthophosphate, and dissolved silica. Additionally, the contribution of streamflow and net nutrient flux for five large subbasins comprising the MARB were determined from streamflow and nutrient fluxes from seven of the aforementioned major subbasins. These five large subbasins are: 1. Lower Mississippi, 2. Upper Mississippi, 3. Ohio/Tennessee, 4. Missouri, and 5. Arkansas/Red.

  10. Scientific Guidance for Rehabilitation of the Chesapeake Bay Ecosystem under the Changing Climate.

    NASA Astrophysics Data System (ADS)

    Boesch, D. F.; Johnson, Z. P.; Li, M.

    2017-12-01

    While the Chesapeake Bay is an estuary and not a marginal sea on the scale of the Baltic Sea or the Gulf of Mexico, it has a complex set of environmental issues and multiple political jurisdictions such that it can serve as a test bed for science-informed management in larger marine systems. In particular, the Chesapeake Bay possesses a relatively advanced effort to ameliorate eutrophication, reduce toxic stresses, rehabilitate critical habitats, and sustainably utilized resources. Furthermore, both scientists and managers are addressing these challenges while now beginning to incorporate the effects of changes in temperature, precipitation and runoff, sea level, ocean boundary conditions, and pH. Increases in temperature and sea level are already apparent and future conditions can be estimated from global model projections, although sea level and ocean exchanges are also affected by variations in Gulf Stream flows and mesoscale climate. Changes in the volume, seasonality and variability in freshwater delivery from the multiple rivers discharging to the bay are harder to project with confidence, but may have pervasive consequences for circulation, reducing nutrient loads to ameliorate eutrophication, biogeochemical processes, and biotic distributions and dynamics. Science is now challenged to inform multiple adaptation strategies, including minimizing the vulnerability of humans and infrastructure, sustaining important tidal wetlands, managing sediment resources, sustaining living resources, redefining achievable ecosystem rehabilitation goals, and achieving shifting goals for nutrient load reductions. At the same time, science will also have to identify effective means to meet these challenges while also reducing greenhouse gas emissions.

  11. MtMOT1.2 is responsible for molybdate supply to Medicago truncatula nodules.

    PubMed

    Gil-Díez, Patricia; Tejada-Jiménez, Manuel; León-Mediavilla, Javier; Wen, Jiangqi; Mysore, Kirankumar S; Imperial, Juan; González-Guerrero, Manuel

    2018-06-25

    Symbiotic nitrogen fixation in legume root nodules requires a steady supply of molybdenum for synthesis of the iron-molybdenum cofactor of nitrogenase. This nutrient has to be provided by the host plant from the soil, crossing several symplastically disconnected compartments through molybdate transporters, including members of the MOT1 family. MtMOT1.2 is a Medicago truncatula MOT1 family member located in the endodermal cells in roots and nodules. Immunolocalization of a tagged MtMOT1.2 indicates that it is associated to the plasma membrane and to intracellular membrane systems, where it would be transporting molybdate towards the cytosol, as indicated in yeast transport assays. Loss-of-function mot1.2-1 mutant showed reduced growth compared to wild-type plants when nitrogen fixation was required, but not when nitrogen was provided as nitrate. While no effect on molybdenum-dependent nitrate reductase activity was observed, nitrogenase activity was severely affected, explaining the observed difference of growth depending on nitrogen source. This phenotype was the result of molybdate not reaching the nitrogen-fixing nodules, since genetic complementation with a wild-type MtMOT1.2 gene or molybdate-fortification of the nutrient solution, both restored wild-type levels of growth and nitrogenase activity. These results support a model in which MtMOT1.2 would mediate molybdate delivery by the vasculature into the nodules. This article is protected by copyright. All rights reserved.

  12. The Mitochondrial 2-Oxoglutarate Carrier Is Part of a Metabolic Pathway That Mediates Glucose- and Glutamine-stimulated Insulin Secretion*

    PubMed Central

    Odegaard, Matthew L.; Joseph, Jamie W.; Jensen, Mette V.; Lu, Danhong; Ilkayeva, Olga; Ronnebaum, Sarah M.; Becker, Thomas C.; Newgard, Christopher B.

    2010-01-01

    Glucose-stimulated insulin secretion from pancreatic islet β-cells is dependent in part on pyruvate cycling through the pyruvate/isocitrate pathway, which generates cytosolic α-ketoglutarate, also known as 2-oxoglutarate (2OG). Here, we have investigated if mitochondrial transport of 2OG through the 2-oxoglutarate carrier (OGC) participates in control of nutrient-stimulated insulin secretion. Suppression of OGC in clonal pancreatic β-cells (832/13 cells) and isolated rat islets by adenovirus-mediated delivery of small interfering RNA significantly decreased glucose-stimulated insulin secretion. OGC suppression also reduced insulin secretion in response to glutamine plus the glutamate dehydrogenase activator 2-amino-2-norbornane carboxylic acid. Nutrient-stimulated increases in glucose usage, glucose oxidation, glutamine oxidation, or ATP:ADP ratio were not affected by OGC knockdown, whereas suppression of OGC resulted in a significant decrease in the NADPH:NADP+ ratio during stimulation with glucose but not glutamine + 2-amino-2-norbornane carboxylic acid. Finally, OGC suppression reduced insulin secretion in response to a membrane-permeant 2OG analog, dimethyl-2OG. These data reveal that the OGC is part of a mechanism of fuel-stimulated insulin secretion that is common to glucose, amino acid, and organic acid secretagogues, involving flux through the pyruvate/isocitrate cycling pathway. Although the components of this pathway must remain intact for appropriate stimulus-secretion coupling, production of NADPH does not appear to be the universal second messenger signal generated by these reactions. PMID:20356834

  13. Boosted Regression Tree Models to Explain Watershed Nutrient Concentrations and Biological Condition

    EPA Science Inventory

    Boosted regression tree (BRT) models were developed to quantify the nonlinear relationships between landscape variables and nutrient concentrations in a mesoscale mixed land cover watershed during base-flow conditions. Factors that affect instream biological components, based on ...

  14. MODELING SEDIMENT-NUTRIENT FLUX AND SEDIMENT OXYGEN DEMAND

    EPA Science Inventory

    Depositional flux of particulate organic matter in bottom sediments affects nutrients cycling at the sediment-water interface and consumes oxygen from the overlying water in streams, lakes, and estuaries. This project deals with analytical modeling of nitrogen and carbon producti...

  15. Stream Restoration to Manage Nutrients in Degraded Watersheds

    EPA Science Inventory

    Historic land-use change can reduce water quality by impairing the ability of stream ecosystems to efficiently process nutrients such as nitrogen. Study results of two streams (Minebank Run and Big Spring Run) affected by urbanization, quarrying, agriculture, and impoundments in...

  16. Spatial Heterogeneity of Soil Nutrients after the Establishment of Caragana intermedia Plantation on Sand Dunes in Alpine Sandy Land of the Tibet Plateau

    PubMed Central

    Li, Qingxue; Jia, Zhiqing; Zhu, Yajuan; Wang, Yongsheng; Li, Hong; Yang, Defu; Zhao, Xuebin

    2015-01-01

    The Gonghe Basin region of the Tibet Plateau is severely affected by desertification. Compared with other desertified land, the main features of this region is windy, cold and short growing season, resulting in relatively difficult for vegetation restoration. In this harsh environment, identification the spatial distribution of soil nutrients and analysis its impact factors after vegetation establishment will be helpful for understanding the ecological relationship between soil and environment. Therefore, in this study, the 12-year-old C. intermedia plantation on sand dunes was selected as the experimental site. Soil samples were collected under and between shrubs on the windward slopes, dune tops and leeward slopes with different soil depth. Then analyzed soil organic matter (SOM), total nitrogen (TN), total phosphorus (TP), total potassium (TK), available nitrogen (AN), available phosphorus (AP) and available potassium (AK). The results showed that the spatial heterogeneity of soil nutrients was existed in C. intermedia plantation on sand dunes. (1) Depth was the most important impact factor, soil nutrients were decreased with greater soil depth. One of the possible reasons is that windblown fine materials and litters were accumulated on surface soil, when they were decomposed, more nutrients were aggregated on surface soil. (2) Topography also affected the distribution of soil nutrients, more soil nutrients distributed on windward slopes. The herbaceous coverage were higher and C. intermedia ground diameter were larger on windward slopes, both of them probably related to the high soil nutrients level for windward slopes. (3) Soil “fertile islands” were formed, and the “fertile islands” were more marked on lower soil nutrients level topography positions, while it decreased towards higher soil nutrients level topography positions. The enrichment ratio (E) for TN and AN were higher than other nutrients, most likely because C. intermedia is a leguminous shrub. PMID:25946170

  17. Spatial Heterogeneity of Soil Nutrients after the Establishment of Caragana intermedia Plantation on Sand Dunes in Alpine Sandy Land of the Tibet Plateau.

    PubMed

    Li, Qingxue; Jia, Zhiqing; Zhu, Yajuan; Wang, Yongsheng; Li, Hong; Yang, Defu; Zhao, Xuebin

    2015-01-01

    The Gonghe Basin region of the Tibet Plateau is severely affected by desertification. Compared with other desertified land, the main features of this region is windy, cold and short growing season, resulting in relatively difficult for vegetation restoration. In this harsh environment, identification the spatial distribution of soil nutrients and analysis its impact factors after vegetation establishment will be helpful for understanding the ecological relationship between soil and environment. Therefore, in this study, the 12-year-old C. intermedia plantation on sand dunes was selected as the experimental site. Soil samples were collected under and between shrubs on the windward slopes, dune tops and leeward slopes with different soil depth. Then analyzed soil organic matter (SOM), total nitrogen (TN), total phosphorus (TP), total potassium (TK), available nitrogen (AN), available phosphorus (AP) and available potassium (AK). The results showed that the spatial heterogeneity of soil nutrients was existed in C. intermedia plantation on sand dunes. (1) Depth was the most important impact factor, soil nutrients were decreased with greater soil depth. One of the possible reasons is that windblown fine materials and litters were accumulated on surface soil, when they were decomposed, more nutrients were aggregated on surface soil. (2) Topography also affected the distribution of soil nutrients, more soil nutrients distributed on windward slopes. The herbaceous coverage were higher and C. intermedia ground diameter were larger on windward slopes, both of them probably related to the high soil nutrients level for windward slopes. (3) Soil "fertile islands" were formed, and the "fertile islands" were more marked on lower soil nutrients level topography positions, while it decreased towards higher soil nutrients level topography positions. The enrichment ratio (E) for TN and AN were higher than other nutrients, most likely because C. intermedia is a leguminous shrub.

  18. The Effect of Nutrient-Based Standards on Competitive Foods in 3 Schools: Potential Savings in Kilocalories and Grams of Fat

    ERIC Educational Resources Information Center

    Snelling, Anastasia M.; Yezek, Jennifer

    2012-01-01

    Background: The study investigated how nutrient standards affected the number of kilocalories and grams of fat and saturated fat in competitive foods offered and sold in 3 high schools. Methods: The study is a quasi-experimental design with 3 schools serving as the units of assignment and analysis. The effect of the nutrient standards was measured…

  19. Growth of mature boreal Norway spruce was not affected by elevated [CO(2)] and/or air temperature unless nutrient availability was improved.

    PubMed

    Sigurdsson, Bjarni D; Medhurst, Jane L; Wallin, Göran; Eggertsson, Olafur; Linder, Sune

    2013-11-01

    The growth responses of mature Norway spruce (Picea abies (L.) Karst.) trees exposed to elevated [CO(2)] (CE; 670-700 ppm) and long-term optimized nutrient availability or elevated air temperature (TE; ±3.9 °C) were studied in situ in northern Sweden in two 3 year field experiments using 12 whole-tree chambers in ca. 40-year-old forest. The first experiment (Exp. I) studied the interactions between CE and nutrient availability and the second (Exp. II) between CE and TE. It should be noted that only air temperature was elevated in Exp. II, while soil temperature was maintained close to ambient. In Exp. I, CE significantly increased the mean annual height increment, stem volume and biomass increment during the treatment period (25, 28, and 22%, respectively) when nutrients were supplied. There was, however, no significant positive CE effect found at the low natural nutrient availability. In Exp. II, which was conducted at the natural site fertility, neither CE nor TE significantly affected height or stem increment. It is concluded that the low nutrient availability (mainly nitrogen) in the boreal forests is likely to restrict their response to the continuous rise in [CO(2)] and/or TE.

  20. Bottom-up nutrient and top-down fish impacts on insect-mediated mercury flux from aquatic ecosystems.

    PubMed

    Jones, Taylor A; Chumchal, Matthew M; Drenner, Ray W; Timmins, Gabrielle N; Nowlin, Weston H

    2013-03-01

    Methyl mercury (MeHg) is one of the most hazardous contaminants in the environment, adversely affecting the health of wildlife and humans. Recent studies have demonstrated that aquatic insects biotransport MeHg and other contaminants to terrestrial consumers, but the factors that regulate the flux of MeHg out of aquatic ecosystems via emergent insects have not been studied. The authors used experimental mesocosms to test the hypothesis that insect emergence and the associated flux of MeHg from aquatic to terrestrial ecosystems is affected by both bottom-up nutrient effects and top-down fish consumer effects. In the present study, nutrient addition led to an increase in MeHg flux primarily by enhancing the biomass of emerging insects whose tissues were contaminated with MeHg, whereas fish decreased MeHg flux primarily by reducing the biomass of emerging insects. Furthermore, the authors found that these factors are interdependent such that the effects of nutrients are more pronounced when fish are absent, and the effects of fish are more pronounced when nutrient concentrations are high. The present study is the first to demonstrate that the flux of MeHg from aquatic to terrestrial ecosystems is strongly enhanced by bottom-up nutrient effects and diminished by top-down consumer effects. Copyright © 2012 SETAC.

  1. Effects of soil nutrient heterogeneity on intraspecific competition in the invasive, clonal plant Alternanthera philoxeroides.

    PubMed

    Zhou, Jian; Dong, Bi-Cheng; Alpert, Peter; Li, Hong-Li; Zhang, Ming-Xiang; Lei, Guang-Chun; Yu, Fei-Hai

    2012-03-01

    Fine-scale, spatial heterogeneity in soil nutrient availability can increase the growth of individual plants, the productivity of plant communities and interspecific competition. If this is due to the ability of plants to concentrate their roots where nutrient levels are high, then nutrient heterogeneity should have little effect on intraspecific competition, especially when there are no genotypic differences between individuals in root plasticity. We tested this hypothesis in a widespread, clonal species in which individual plants are known to respond to nutrient heterogeneity. Plants derived from a single clone of Alternanthera philoxeroides were grown in the greenhouse at low or high density (four or 16 plants per 27·5 × 27·5-cm container) with homogeneous or heterogeneous availability of soil nutrients, keeping total nutrient availability per container constant. After 9 weeks, measurements of size, dry mass and morphology were taken. Plants grew more in the heterogeneous than in the homogeneous treatment, showing that heterogeneity promoted performance; they grew less in the high- than in the low-density treatment, showing that plants competed. There was no interactive effect of nutrient heterogeneity and plant density, supporting the hypothesis that heterogeneity does not affect intraspecific competition in the absence of genotypic differences in plasticity. Treatments did not affect morphological characteristics such as specific leaf area or root/shoot ratio. Results indicate that fine-scale, spatial heterogeneity in the availability of soil nutrients does not increase competition when plants are genetically identical, consistent with the suggestion that effects of heterogeneity on competition depend upon differences in plasticity between individuals. Heterogeneity is only likely to increase the spread of monoclonal, invasive populations such as that of A. philoxeroides in China.

  2. Does meperidine analgesia affect the incidence of obstetric lacerations at vaginal delivery?

    PubMed

    Mizrachi, Yossi; Leytes, Sophia; Levy, Michal; Ginath, Shimon; Bar, Jacob; Ezri, Tiberiu; Kovo, Michal

    2018-03-01

    To study whether meperidine analgesia affects the incidence of obstetric lacerations at normal vaginal deliveries. A retrospective cohort study of all women with term vertex singleton pregnancies, who underwent normal vaginal deliveries, in a single tertiary hospital, between 2011 and 2015, was performed. The incidence of various obstetric lacerations was compared between deliveries with meperidine analgesia and deliveries with no analgesia. Deliveries with epidural analgesia and instrumental deliveries were excluded. An intravenous infusion of 75 mg of meperidine was administered together with 25 mg of promethazine. A multivariate logistic regression analysis was performed to assess the association between meperidine analgesia and obstetric lacerations, after controlling for confounders. Overall, 5227 (91.8%) deliveries with no analgesia and 466 (8.1%) deliveries with meperidine analgesia were included. Meperidine analgesia was associated with a decreased risk of first- and second-degree perineal lacerations (adjusted OR = 0.63, 95% CI = 0.49-0.81), and a decreased risk of any suturing (adjusted OR = 0.73, 95% CI = 0.59-0.91), after controlling for confounders. Meperidine analgesia did not affect the risk of severe perineal lacerations or episiotomies. Meperidine analgesia may have a protective effect against first- and second-degree perineal lacerations.

  3. A Dietary Feedback System for the Delivery of Consistent Personalized Dietary Advice in the Web-Based Multicenter Food4Me Study.

    PubMed

    Forster, Hannah; Walsh, Marianne C; O'Donovan, Clare B; Woolhead, Clara; McGirr, Caroline; Daly, E J; O'Riordan, Richard; Celis-Morales, Carlos; Fallaize, Rosalind; Macready, Anna L; Marsaux, Cyril F M; Navas-Carretero, Santiago; San-Cristobal, Rodrigo; Kolossa, Silvia; Hartwig, Kai; Mavrogianni, Christina; Tsirigoti, Lydia; Lambrinou, Christina P; Godlewska, Magdalena; Surwiłło, Agnieszka; Gjelstad, Ingrid Merethe Fange; Drevon, Christian A; Manios, Yannis; Traczyk, Iwona; Martinez, J Alfredo; Saris, Wim H M; Daniel, Hannelore; Lovegrove, Julie A; Mathers, John C; Gibney, Michael J; Gibney, Eileen R; Brennan, Lorraine

    2016-06-30

    Despite numerous healthy eating campaigns, the prevalence of diets high in saturated fatty acids, sugar, and salt and low in fiber, fruit, and vegetables remains high. With more people than ever accessing the Internet, Web-based dietary assessment instruments have the potential to promote healthier dietary behaviors via personalized dietary advice. The objectives of this study were to develop a dietary feedback system for the delivery of consistent personalized dietary advice in a multicenter study and to examine the impact of automating the advice system. The development of the dietary feedback system included 4 components: (1) designing a system for categorizing nutritional intakes; (2) creating a method for prioritizing 3 nutrient-related goals for subsequent targeted dietary advice; (3) constructing decision tree algorithms linking data on nutritional intake to feedback messages; and (4) developing personal feedback reports. The system was used manually by researchers to provide personalized nutrition advice based on dietary assessment to 369 participants during the Food4Me randomized controlled trial, with an automated version developed on completion of the study. Saturated fatty acid, salt, and dietary fiber were most frequently selected as nutrient-related goals across the 7 centers. Average agreement between the manual and automated systems, in selecting 3 nutrient-related goals for personalized dietary advice across the centers, was highest for nutrient-related goals 1 and 2 and lower for goal 3, averaging at 92%, 87%, and 63%, respectively. Complete agreement between the 2 systems for feedback advice message selection averaged at 87% across the centers. The dietary feedback system was used to deliver personalized dietary advice within a multi-country study. Overall, there was good agreement between the manual and automated feedback systems, giving promise to the use of automated systems for personalizing dietary advice. Clinicaltrials.gov NCT01530139; https://clinicaltrials.gov/ct2/show/NCT01530139 (Archived by WebCite at http://www.webcitation.org/6ht5Dgj8I).

  4. Nutrient sources and transport in the Missouri River Basin, with emphasis on the effects of irrigation and reservoirs

    USGS Publications Warehouse

    Brown, J.B.; Sprague, L.A.; Dupree, J.A.

    2011-01-01

    SPAtially Referenced Regressions On Watershed attributes (SPARROW) models were used to relate instream nutrient loads to sources and factors influencing the transport of nutrients in the Missouri River Basin. Agricultural inputs from fertilizer and manure were the largest nutrient sources throughout a large part of the basin, although atmospheric and urban inputs were important sources in some areas. Sediment mobilized from stream channels was a source of phosphorus in medium and larger streams. Irrigation on agricultural land was estimated to decrease the nitrogen load reaching the Mississippi River by as much as 17%, likely as a result of increased anoxia and denitrification in the soil zone. Approximately 16% of the nitrogen load and 33% of the phosphorus load that would have otherwise reached the Mississippi River was retained in reservoirs and lakes throughout the basin. Nearly half of the total attenuation occurred in the eight largest water bodies. Unlike the other major tributary basins, nearly the entire instream nutrient load leaving the outlet of the Platte and Kansas River subbasins reached the Mississippi River. Most of the larger reservoirs and lakes in the Platte River subbasin are upstream of the major sources, whereas in the Kansas River subbasin, most of the source inputs are in the southeast part of the subbasin where characteristics of the area and proximity to the Missouri River facilitate delivery of nutrients to the Mississippi River.

  5. Quantitative Assessment of Agricultural Runoff and Soil Erosion Using Mathematical Modeling: Applications in the Mediterranean Region

    NASA Astrophysics Data System (ADS)

    Arhonditsis, G.; Giourga, C.; Loumou, A.; Koulouri, M.

    2002-09-01

    Three mathematical models, the runoff curve number equation, the universal soil loss equation, and the mass response functions, were evaluated for predicting nonpoint source nutrient loading from agricultural watersheds of the Mediterranean region. These methodologies were applied to a catchment, the gulf of Gera Basin, that is a typical terrestrial ecosystem of the islands of the Aegean archipelago. The calibration of the model parameters was based on data from experimental plots from which edge-of-field losses of sediment, water runoff, and nutrients were measured. Special emphasis was given to the transport of dissolved and solid-phase nutrients from their sources in the farmers' fields to the outlet of the watershed in order to estimate respective attenuation rates. It was found that nonpoint nutrient loading due to surface losses was high during winter, the contribution being between 50% and 80% of the total annual nutrient losses from the terrestrial ecosystem. The good fit between simulated and experimental data supports the view that these modeling procedures should be considered as reliable and effective methodological tools in Mediterranean areas for evaluating potential control measures, such as management practices for soil and water conservation and changes in land uses, aimed at diminishing soil loss and nutrient delivery to surface waters. Furthermore, the modifications of the general mathematical formulations and the experimental values of the model parameters provided by the study can be used in further application of these methodologies in watersheds with similar characteristics.

  6. Arctic water tracks retain phosphorus and transport ammonium

    NASA Astrophysics Data System (ADS)

    Harms, T.; Cook, C. L.; Wlostowski, A. N.; Godsey, S.; Gooseff, M. N.

    2017-12-01

    Hydrologic flowpaths propagate biogeochemical signals among adjacent ecosystems, but reactions may attenuate signals by retaining, removing, or transforming dissolved and suspended materials. The theory of nutrient spiraling describes these simultaneous reaction and transport processes, but its application has been limited to stream channels. We applied nutrient spiraling theory to water tracks, zero-order channels draining Arctic hillslopes that contain perennially saturated soils and flow at the surface either perennially or in response to precipitation. In the Arctic, experimental warming results in increased availability of nitrogen, the limiting nutrient for hillslope vegetation at the study site, which may be delivered to aquatic ecosystems by water tracks. Increased intensity of rain events, deeper snowpack, earlier snowmelt, and increasing thaw depth resulting from climate change might support increased transport of nutrients, but the reactive capacity of hillslope flowpaths, including sorption and uptake by plants and microbes, could counter transport to regulate solute flux. Characteristics of flowpaths might influence the opportunity for reaction, where slower flowpaths increase the contact time between solutes and soils or roots. We measured nitrogen and phosphorus uptake and transient storage of water tracks through the growing season and found that water tracks retain inorganic phosphorus, but transport ammonium. Nutrient uptake was unrelated to transient storage, suggesting high capacity for nutrient retention by shallow organic soils and vegetation. These observations indicate that increased availability of ammonium, the biogeochemical signal of warming tundra, is propagated by hillslope flowpaths, whereas water tracks attenuate delivery of phosphorus to aquatic ecosystems, where its availability typically limits production.

  7. Nutrient Sources and Transport in the Missouri River Basin, with Emphasis on the Effects of Irrigation and Reservoirs1

    PubMed Central

    Brown, Juliane B; Sprague, Lori A; Dupree, Jean A

    2011-01-01

    Abstract SPAtially Referenced Regressions On Watershed attributes (SPARROW) models were used to relate instream nutrient loads to sources and factors influencing the transport of nutrients in the Missouri River Basin. Agricultural inputs from fertilizer and manure were the largest nutrient sources throughout a large part of the basin, although atmospheric and urban inputs were important sources in some areas. Sediment mobilized from stream channels was a source of phosphorus in medium and larger streams. Irrigation on agricultural land was estimated to decrease the nitrogen load reaching the Mississippi River by as much as 17%, likely as a result of increased anoxia and denitrification in the soil zone. Approximately 16% of the nitrogen load and 33% of the phosphorus load that would have otherwise reached the Mississippi River was retained in reservoirs and lakes throughout the basin. Nearly half of the total attenuation occurred in the eight largest water bodies. Unlike the other major tributary basins, nearly the entire instream nutrient load leaving the outlet of the Platte and Kansas River subbasins reached the Mississippi River. Most of the larger reservoirs and lakes in the Platte River subbasin are upstream of the major sources, whereas in the Kansas River subbasin, most of the source inputs are in the southeast part of the subbasin where characteristics of the area and proximity to the Missouri River facilitate delivery of nutrients to the Mississippi River. PMID:22457581

  8. Evaluating Best Management Practices for ephemeral channel protection following forest harvest in the Cumberland Plateau - preliminary findings

    Treesearch

    Emma L. Witt; Christopher D. Barton; Jeffrey W. Stringer; Daniel W. Bowker; Randall K. Kolka

    2011-01-01

    Most states in the United States have established forestry best management practices to protect water quality and maintain aquatic habitat in streams. However, guidelines are generally focused on minimizing impacts to perennial streams. Ephemeral channels (or streams), which function as important delivery systems for carbon, nutrients, and sediment to perennial streams...

  9. Computer Simulations of the Tumor Vasculature: Applications to Interstitial Fluid Flow, Drug Delivery, and Oxygen Supply.

    PubMed

    Welter, Michael; Rieger, Heiko

    2016-01-01

    Tumor vasculature, the blood vessel network supplying a growing tumor with nutrients such as oxygen or glucose, is in many respects different from the hierarchically organized arterio-venous blood vessel network in normal tissues. Angiogenesis (the formation of new blood vessels), vessel cooption (the integration of existing blood vessels into the tumor vasculature), and vessel regression remodel the healthy vascular network into a tumor-specific vasculature. Integrative models, based on detailed experimental data and physical laws, implement, in silico, the complex interplay of molecular pathways, cell proliferation, migration, and death, tissue microenvironment, mechanical and hydrodynamic forces, and the fine structure of the host tissue vasculature. With the help of computer simulations high-precision information about blood flow patterns, interstitial fluid flow, drug distribution, oxygen and nutrient distribution can be obtained and a plethora of therapeutic protocols can be tested before clinical trials. This chapter provides an overview over the current status of computer simulations of vascular remodeling during tumor growth including interstitial fluid flow, drug delivery, and oxygen supply within the tumor. The model predictions are compared with experimental and clinical data and a number of longstanding physiological paradigms about tumor vasculature and intratumoral solute transport are critically scrutinized.

  10. The micro and macro of nutrients across biological scales.

    PubMed

    Warne, Robin W

    2014-11-01

    During the past decade, we have gained new insights into the profound effects that essential micronutrients and macronutrients have on biological processes ranging from cellular function, to whole-organism performance, to dynamics in ecological communities, as well as to the structure and function of ecosystems. For example, disparities between intake and organismal requirements for specific nutrients are known to strongly affect animal physiological performance and impose trade-offs in the allocations of resources. However, recent findings have demonstrated that life-history allocation trade-offs and even microevolutionary dynamics may often be a result of molecular-level constraints on nutrient and metabolic processing, in which limiting reactants are routed among competing biochemical pathways. In addition, recent work has shown that complex ecological interactions between organismal physiological states such as exposure to environmental stressors and infectious pathogens can alter organismal requirements for, and, processing of, nutrients, and even alter subsequent nutrient cycling in ecosystems. Furthermore, new research is showing that such interactions, coupled with evolutionary and biogeographical constraints on the biosynthesis and availability of essential nutrients and micronutrients play an important, but still under-studied role in the structuring and functioning of ecosystems. The purpose of this introduction to the symposium "The Micro and Macro of Nutrient Effects in Animal Physiology and Ecology" is to briefly review and highlight recent research that has dramatically advanced our understanding of how nutrients in their varied forms profoundly affect and shape ecological and evolutionary processes. © The Author 2014. Published by Oxford University Press on behalf of the Society for Integrative and Comparative Biology. All rights reserved. For permissions please email: journals.permissions@oup.com.

  11. Analyzing B-vitamins in human milk: methodological approaches

    USDA-ARS?s Scientific Manuscript database

    According to the World Health Organization (WHO) infants should be exclusively breastfed for the first six months of life. However, there is insufficient information about the concentration of nutrients in human milk. For some nutrients, including B-vitamins, maternal intake affects their concentrat...

  12. Growth and nutrient status of black spruce seedlings as affected by water table depth

    Treesearch

    Miroslaw M. Czapowskyj; Robert V. Rourke; Walter J. Grant; Walter J. Grant

    1986-01-01

    The objective of this study was to determine the effect of different soil water table levels on growth, biomass production, and nutrient accumulation in black spruce seedlings growing under greenhouse conditions over three growing seasons after transplanting.

  13. Urban Effects on Microbial Processes and Food Webs in Coastal Watershed Streams

    EPA Science Inventory

    We conducted a stream survey in the Narragansett Bay Watershed that targeted a gradient of development intensity and examined how associated changes in nutrients, carbon, and stressors affected periphyton and macroinvertebrates. Concentrations of nutrients, cations, and anions we...

  14. Comparison of Two Spectrophotometric Techniques for Nutrients Analyses in Water Samples

    NASA Astrophysics Data System (ADS)

    Bartošová, Alica; Michalíková, Anna; Sirotiak, Maroš; Soldán, Maroš

    2013-01-01

    The aim of this contribution is to compare two common techniques for determining the concentrations of nitrate, nitrite, ammonium and phosphates in surface water and groundwater. Excess of these nutrients in water can directly affect human health (e.g. methemoglobinaemia) or indirectly through the products of secondary pollution - eutrophication (e.g. cyanotoxins, emanation of hydrogen sulphide, mercaptanes, methane...). Negative impact of nutrients excess in surface water often causes the destruction of water ecosystems, and therefore, common substances of these elements must be monitored and managed. For these experiments two spectrophotometric techniques - ultraviolet spectrophotometry and nutrient photometry were used. These techniques are commonly used for quick and simple analyses of nutrients in waste water. There are calibration curves for each nutrient and for determination of their concentration.

  15. Stoichiometry and climatic stress drive respiration and nutrient dynamics of beech litter decomposition

    NASA Astrophysics Data System (ADS)

    Keiblinger, Katharina Maria; Hämmerle, Ieda; Zechmeister-Boltenstern, Sophie

    2010-05-01

    Little is known about how the variance in resources in terms of carbon (C), nitrogen (N), phosphorus (P) ratios affects respiration and nutrient dynamics. To elucidate how resource quantity and stoichiometry affect the decomposition process of beech (Fagus sylvatica) litter a terrestrial microcosm experiment was conducted. Our aim was to follow changes of beech litter stoichiometry and biogeochemical processes, and to quantify element losses as affected by temperature and moisture extremes. In addition to gaseous element losses (CO2) we examined the release of nutrients prone to leaching and the importance of environmental controls. We addressed mechanisms and pathways of carbon, nitrogen and phosphorus losses. In our experiment sterilised dried leaves were inoculated with a litter-soil suspension from a beech forest in order to ensure similar starting conditions. Beech litter from different Austrian sites covering C:N ratios from 45 to 66 and C:P ratios from 652 to 1467 were incubated at 15°C for six months. The water content was adjusted to 60% at regular intervals to keep the moisture constant. To monitor transient and persistent influences of environmental stress, the microcosms were subject to extreme changes in temperature (+30°C and -20°C) and moisture (draught) after an incubation time of three months. Litter stoichiometries (C:N, C:P) turned out to be strong predictors for respiration, and nitrogen, and phosphorous losses. (i) Litter with narrow litter C:nutrient ratios decomposed faster than litter with wider litter C:nutrient ratios; and therefore showed higher respiration rates. (ii) Increased nutrient losses as leachates were observed for high quality leaf litter i.e. inorganic nitrogen losses for sites with narrow litter C:N ratios and phosphate was released more quickly in sites with narrow C:P ratios. There was a strong functional response of the microbial community to environmental extremes. Respiration increased upon temperature extremes, especially in the litter with highest C:P ratio. A persistent effect of temperature extremes on NH4 and NO3 concentrations was observed for three months after stress application. However, the effect on PO4 concentrations was only transient. Environmental conditions had a strong affect on nutrient losses but only a minor affect on microbial carbon Cmic and microbial nitrogen Nmic. The impact of environmental stress (heat or freezing) on microbes in terms of Cmic, Nmic and C:Nmic was strongest in sites with narrow litter C:N ratios. Our results indicate a similar stoichiometric demand of microbes, with temporal changes which results in differences in nutrient cycling on substrates with different C:N:P ratios.

  16. Seed removal by scatter-hoarding rodents: the effects of tannin and nutrient concentration.

    PubMed

    Wang, Bo; Yang, Xiaolan

    2015-04-01

    The mutualistic interaction between scatter-hoarding rodents and seed plants have a long co-evolutionary history. Plants are believed to have evolved traits that influence the foraging behavior of rodents, thus increasing the probability of seed removal and caching, which benefits the establishment of seedlings. Tannin and nutrient content in seeds are considered among the most essential factors in this plant-animal interaction. However, most previous studies used different species of plant seeds, rendering it difficult to tease apart the relative effect of each single nutrient on rodent foraging behavior due to confounding combinations of nutrient contents across seed species. Hence, to further explore how tannin and different nutritional traits of seed affect scatter-hoarding rodent foraging preferences, we manipulated tannin, fat, protein and starch content levels, and also seed size levels by using an artificial seed system. Our results showed that both tannin and various nutrients significantly affected rodent foraging preferences, but were also strongly affected by seed size. In general, rodents preferred to remove seeds with less tannin. Fat addition could counteract the negative effect of tannin on seed removal by rodents, while the effect of protein addition was weaker. Starch by itself had no effect, but it interacted with tannin in a complex way. Our findings shed light on the effects of tannin and nutrient content on seed removal by scatter-hoarding rodents. We therefore, believe that these and perhaps other seed traits should interactively influence this important plant-rodent interaction. However, how selection operates on seed traits to counterbalance these competing interests/factors merits further study. Copyright © 2015 Elsevier B.V. All rights reserved.

  17. Entisol land characteristics with and without cover crop (Mucuna bracteata) on rubber plantation

    NASA Astrophysics Data System (ADS)

    Sakiah; Sembiring, M.; Hasibuan, J.

    2018-02-01

    Optimal nutrient delivery is one way to improve the quality and quantity of crop production. This is because the crops needs for nutrient is quite high, while the soil capacity in providing nutrients is limited. In addition to fertilization, nutrients can be given in the form of added organic material or planted as cover crop. The research took place from April to August 2016 in Bandar Pinang, Bandar Sumatera Indonesia Ltd. (SIPEF Group) plantation, with survey method. Soil samples were taken based on: Topography (flat and slope 15-30%), cover crop (with or without Mucuna bracteata) and plant age (seedling periods 1, 2 and 3). The soil sample is taken composite by zig zag method. The observed parameters were organic matter, N total, soil texture, bulk density and infiltration rate. Mucuna bracteata planting increased the contain of soil organic matter by 30.43% in flat area and 53.33% in hilly area, amount of N total soil by 27.27% in flat area and 7.69% at hilly area, bulk density 3.73 % In flat area and 0.41% in hilly area, soil infiltration by 48.88% with sandy clay dominant soil texture.

  18. Response of the soil microbial community and soil nutrient bioavailability to biomass harvesting and reserve tree retention in northern Minnesota aspen-dominated forests

    Treesearch

    Tera E. Lewandowski; Jodi A. Forrester; David J. Mladenoff; Anthony W. D' Amato; Brian J. Palik

    2016-01-01

    Intensive forest biomass harvesting, or the removal of harvesting slash (woody debris from tree branches and tops) for use as biofuel, has the potential to negatively affect the soil microbial community (SMC) due to loss of carbon and nutrient inputs from the slash, alteration of the soil microclimate, and increased nutrient leaching. These effects could result in...

  19. The use of acoustic doppler meters to estimate sediment and nutrient concentrations in freshwater inflows to Texas coastal ecosystems

    Treesearch

    Zullmar Lucena; Micheal Lee

    2016-01-01

    Excessive sediment and nutrient loading are among the leading causes of impairment in water bodies of the United States due to their effect on biologic productivity, water quality, and aquatic food webs. Understanding the nutrient and suspended sediment loads affecting estuarine waters is fundamental to the assessment of the physical, chemical, and biological processes...

  20. Effects of hurricane-felled tree trunks on soil carbon, nitrogen, microbial biomass, and root length in a wet tropical forest

    Treesearch

    D. Jean Lodge; Dirk Winter; Grizelle Gonzalez; Naomi Clum

    2016-01-01

    Decaying coarse woody debris can affect the underlying soil either by augmenting nutrients that can be exploited by tree roots, or by diminishing nutrient availability through stimulation of microbial nutrient immobilization. We analyzed C, N, microbial biomass C and root length in closely paired soil samples taken under versus 20–50 cm away from large trunks of two...

  1. Soil moisture and biogeochemical factors influence the distribution of annual Bromus species

    USGS Publications Warehouse

    Belnap, Jayne; Stark, John Thomas; Rau, Benjamin; Allen, Edith B.; Phillips, Sue

    2016-01-01

    Abiotic factors have a strong influence on where annual Bromus species are found. At the large regional scale, temperature and precipitation extremes determine the boundaries of Bromusoccurrence. At the more local scale, soil characteristics and climate influence distribution, cover, and performance. In hot, dry, summer-rainfall-dominated deserts (Sonoran, Chihuahuan), little or noBromus is found, likely due to timing or amount of soil moisture relative to Bromus phenology. In hot, winter-rainfall-dominated deserts (parts of the Mojave Desert), Bromus rubens is widespread and correlated with high phosphorus availability. It also responds positively to additions of nitrogen alone or with phosphorus. On the Colorado Plateau, with higher soil moisture availability, factors limiting Bromus tectorum populations vary with life stage: phosphorus and water limit germination, potassium and the potassium/magnesium ratio affect winter performance, and water and potassium/magnesium affect spring performance. Controlling nutrients also change with elevation. In cooler deserts with winter precipitation (Great Basin, Columbia Plateau) and thus even greater soil moisture availability, B. tectorum populations are controlled by nitrogen, phosphorus, or potassium. Experimental nitrogen additions stimulate Bromus performance. The reason for different nutrients limiting in dissimilar climatic regions is not known, but it is likely that site conditions such as soil texture (as it affects water and nutrient availability), organic matter, and/or chemistry interact in a manner that regulates nutrient availability and limitations. Under future drier, hotter conditions,Bromus distribution is likely to change due to changes in the interaction between moisture and nutrient availability.

  2. Effects of Liming on Forage Availability and Nutrient Content in a Forest Impacted by Acid Rain

    PubMed Central

    Pabian, Sarah E.; Ermer, Nathan M.; Tzilkowski, Walter M.; Brittingham, Margaret C.

    2012-01-01

    Acidic deposition and subsequent forest soil acidification and nutrient depletion can affect negatively the growth, health and nutrient content of vegetation, potentially limiting the availability and nutrient content of forage for white-tailed deer (Odocoileus virginianus) and other forest herbivores. Liming is a mitigation technique that can be used to restore forest health in acidified areas, but little is known about how it affects the growth or nutrient content of deer forage. We examined the effects of dolomitic limestone application on the growth and chemical composition of understory plants in an acidified forest in central Pennsylvania, with a focus on vegetative groups included as white-tailed deer forage. We used a Before-After-Control-Impact study design with observations 1 year before liming and up to 5 years post-liming on 2 treated and 2 untreated 100-ha sites. Before liming, forage availability and several nutrients were below levels considered optimal for white-tailed deer, and many vegetative characteristics were related to soil chemistry. We observed a positive effect of liming on forb biomass, with a 2.7 fold increase on limed sites, but no biomass response in other vegetation groups. We observed positive effects of liming on calcium and magnesium content and negative effects on aluminum and manganese content of several plant groups. Responses to liming by forbs and plant nutrients show promise for improving vegetation health and forage quality and quantity for deer. PMID:22761890

  3. Effects of liming on forage availability and nutrient content in a forest impacted by acid rain.

    PubMed

    Pabian, Sarah E; Ermer, Nathan M; Tzilkowski, Walter M; Brittingham, Margaret C

    2012-01-01

    Acidic deposition and subsequent forest soil acidification and nutrient depletion can affect negatively the growth, health and nutrient content of vegetation, potentially limiting the availability and nutrient content of forage for white-tailed deer (Odocoileus virginianus) and other forest herbivores. Liming is a mitigation technique that can be used to restore forest health in acidified areas, but little is known about how it affects the growth or nutrient content of deer forage. We examined the effects of dolomitic limestone application on the growth and chemical composition of understory plants in an acidified forest in central Pennsylvania, with a focus on vegetative groups included as white-tailed deer forage. We used a Before-After-Control-Impact study design with observations 1 year before liming and up to 5 years post-liming on 2 treated and 2 untreated 100-ha sites. Before liming, forage availability and several nutrients were below levels considered optimal for white-tailed deer, and many vegetative characteristics were related to soil chemistry. We observed a positive effect of liming on forb biomass, with a 2.7 fold increase on limed sites, but no biomass response in other vegetation groups. We observed positive effects of liming on calcium and magnesium content and negative effects on aluminum and manganese content of several plant groups. Responses to liming by forbs and plant nutrients show promise for improving vegetation health and forage quality and quantity for deer.

  4. Digital data used to relate nutrient inputs to water quality in the Chesapeake Bay watershed

    USGS Publications Warehouse

    Brakebill, John W.; Preston, Stephen D.

    1999-01-01

    Digital data sets were compiled by the U. S. Geological Survey (USGS) and used as input for a collection of Spatially Referenced Regressions On Watershed attributes for the Chesapeake Bay region. These regressions relate streamwater loads to nutrient sources and the factors that affect the transport of these nutrients throughout the watershed. A digital segmented network based on watershed boundaries serves as the primary foundation for spatially referencing total nitrogen and total phosphorus source and land-surface characteristic data sets within a Geographic Information System. Digital data sets of atmospheric wet deposition of nitrate, point-source discharge locations, land cover, and agricultural sources such as fertilizer and manure were created and compiled from numerous sources and represent nitrogen and phosphorus inputs. Some land-surface characteristics representing factors that affect the transport of nutrients include land use, land cover, average annual precipitation and temperature, slope, and soil permeability. Nutrient input and land-surface characteristic data sets merged with the segmented watershed network provide the spatial detail by watershed segment required by the models. Nutrient stream loads were estimated for total nitrogen, total phosphorus, nitrate/nitrite, amonium, phosphate, and total suspended soilds at as many as 109 sites within the Chesapeake Bay watershed. The total nitrogen and total phosphorus load estimates are the dependent variables for the regressions and were used for model calibration. Other nutrient-load estimates may be used for calibration in future applications of the models.

  5. Neuronal Control of Metabolism through Nutrient-Dependent Modulation of Tracheal Branching

    PubMed Central

    Linneweber, Gerit A.; Jacobson, Jake; Busch, Karl Emanuel; Hudry, Bruno; Christov, Christo P.; Dormann, Dirk; Yuan, Michaela; Otani, Tomoki; Knust, Elisabeth; de Bono, Mario; Miguel-Aliaga, Irene

    2014-01-01

    Summary During adaptive angiogenesis, a key process in the etiology and treatment of cancer and obesity, the vasculature changes to meet the metabolic needs of its target tissues. Although the cues governing vascular remodeling are not fully understood, target-derived signals are generally believed to underlie this process. Here, we identify an alternative mechanism by characterizing the previously unrecognized nutrient-dependent plasticity of the Drosophila tracheal system: a network of oxygen-delivering tubules developmentally akin to mammalian blood vessels. We find that this plasticity, particularly prominent in the intestine, drives—rather than responds to—metabolic change. Mechanistically, it is regulated by distinct populations of nutrient- and oxygen-responsive neurons that, through delivery of both local and systemic insulin- and VIP-like neuropeptides, sculpt the growth of specific tracheal subsets. Thus, we describe a novel mechanism by which nutritional cues modulate neuronal activity to give rise to organ-specific, long-lasting changes in vascular architecture. PMID:24439370

  6. Dust in the Earth system: the biogeochemical linking of land, air and sea.

    PubMed

    Ridgwell, Andy J

    2002-12-15

    Understanding the response of the Earth's climate system to anthropogenic perturbation has been a pressing priority for society since the late 1980s. However, recent years have seen a major paradigm shift in how such an understanding can be reached. Climate change demands analysis within an integrated 'Earth-system' framework, taken to encompass the suite of interacting physical, chemical, biological and human processes that, in transporting and transforming materials and energy, jointly determine the conditions for life on the whole planet. This is a highly complex system, characterized by multiple nonlinear responses and thresholds, with linkages often between apparently disparate components. The interconnected nature of the Earth system is wonderfully illustrated by the diverse roles played by atmospheric transport of mineral 'dust', particularly in its capacity as a key pathway for the delivery of nutrients essential to plant growth, not only on land, but perhaps more importantly, in the ocean. Dust therefore biogeochemically links land, air and sea. This paper reviews the biogeochemical role of mineral dust in the Earth system and its interaction with climate, and, in particular, the potential importance of both past and possible future changes in aeolian delivery of the micro-nutrient iron to the ocean. For instance, if, in the future, there was to be a widespread stabilization of soils for the purpose of carbon sequestration on land, a reduction in aeolian iron supply to the open ocean would occur. The resultant weakening of the oceanic carbon sink could potentially offset much of the carbon sequestered on land. In contrast, during glacial times, enhanced dust supply to the ocean could have 'fertilized' the biota and driven atmospheric CO(2) lower. Dust might even play an active role in driving climatic change; since changes in dust supply may affect climate, and changes in climate, in turn, influence dust, a 'feedback loop' is formed. Possible feedback mechanisms are identified, recognition of whose operation could be crucial to our understanding of major climatic transitions over the past few million years.

  7. Influence of plant invasion on seed chemistry of winterfat, green rabbitbrush, freckled milkvetch, indian ricegrass and cheatgrass

    USDA-ARS?s Scientific Manuscript database

    Plant invasions have proven detrimental to numerous ecosystem processes; however, limited information exists on how plant invasions affect seed nutrients. We quantified nutrients in seeds of Indian ricegrass (Achnatherum hymenoides), green rabbitbrush (Chrysothamnus viscidiflorus), winterfat (Krasch...

  8. Carrot, Corn, Lettuce and Soybean Nutrient Contents are Affected by Biochar

    EPA Science Inventory

    Biochar, the carbon-rich material remaining after pyrolysis of cellulosic and manure feedstocks, has the potential as a soil amendment to sequester carbon and to improve soil water-holding and nutrient properties- thereby enhancing plant growth. However, biochar produced from so...

  9. Significance of Plankton Community Structure and Nutrient Availability for the Control of Dinoflagellate Blooms by Parasites: A Modeling Approach

    PubMed Central

    Alves-de-Souza, Catharina; Pecqueur, David; Le Floc’h, Emilie; Mas, Sébastien; Roques, Cécile; Mostajir, Behzad; Vidussi, Franscesca; Velo-Suárez, Lourdes; Sourisseau, Marc; Fouilland, Eric; Guillou, Laure

    2015-01-01

    Dinoflagellate blooms are frequently observed under temporary eutrophication of coastal waters after heavy rains. Growth of these opportunistic microalgae is believed to be promoted by sudden input of nutrients and the absence or inefficiency of their natural enemies, such as grazers and parasites. Here, numerical simulations indicate that increasing nutrient availability not only promotes the formation of dinoflagellate blooms but can also stimulate their control by protozoan parasites. Moreover, high abundance of phytoplankton other than dinoflagellate hosts might have a significant dilution effect on the control of dinoflagellate blooms by parasites, either by resource competition with dinoflagellates (thus limiting the number of hosts available for infection) or by affecting numerical-functional responses of grazers that consume free-living parasite stages. These outcomes indicate that although both dinoflagellates and their protozoan parasites are directly affected by nutrient availability, the efficacy of the parasitic control of dinoflagellate blooms under temporary eutrophication depends strongly on the structure of the plankton community as a whole. PMID:26030411

  10. The effects of human milk fortification on nutrients and milk properties.

    PubMed

    Donovan, R; Kelly, S G; Prazad, P; Talaty, P N; Lefaiver, C; Hastings, M L; Everly, D N

    2017-01-01

    To investigate the effects of fortification and storage on nutrients and properties of various human milk (HM) types. Mother's own milk (MOM) and pasteurized donor human milk (DHM; n=118) were analyzed pre- and post fortification with Enfamil and Similac human milk fortifier (EHMF and SHMF) before and after 24 h of refrigerated storage. Milk fortified with SHMF had significantly greater osmolality, pH and lipase activity than EHMF. Changes in protein, pH and osmolality following refrigerated storage differed between fortifiers. When milk type was factored into the analysis, protein and lipase activity changes in fresh MOM differed significantly from DHM and frozen MOM. Analysis of UNF HM found higher protein levels in preterm vs term samples and in MOM vs DHM. Nutrient composition of HM varies significantly by milk type. Although fortifiers enhance select nutrients, each has the potential to affect HM properties in a unique way and these affects may vary by milk type.

  11. Effectiveness of Vegetated Drainage Ditches for Domestic Sewage Effluent Mitigation.

    PubMed

    Kumwimba, Mathieu Nsenga; Zhu, Bo

    2017-05-01

    Plant species have an important role in eco-ditches; however, the Michaelis-Menten kinetic parameters of nutrient uptake, growth rate and purification efficiency of ditch plants and their influences on domestic sewage treatment efficiency are still unclear. Growth rates of all nine species, but especially Lemna gibba, Cladophora and Myriophyllum verticillatum were best in undiluted domestic sewage as opposed to a mixture of domestic sewage. Performance of species to accumulate nutrients was not only species-specific, but was also affected by both sewage treatments. Removal efficiency of nutrients was dependent on both plant species and treatment. Uptake kinetic parameters were significantly affected by both nutrient form and plant species. The maximum uptake rate (Vmax) of NH 4 -N was higher than NO 3 -N. Similarly, Km values for NH 4 -N were greater than NO 3 -N. These results could be used to identify plants for sewage treatment efficiency and enhance water quality in eco-ditch treatment systems.

  12. Does infection tilt the scales? Disease effects on the mass balance of an invertebrate nutrient recycler.

    PubMed

    Narr, Charlotte F; Frost, Paul C

    2015-12-01

    While parasites are increasingly recognized as important components of ecosystems, we currently know little about how they alter ecosystem nutrient availability via host-mediated nutrient cycling. We examined whether infection alters the flow of nutrients through hosts and whether such effects depend upon host diet quality. To do so, we compared the mass specific nutrient (i.e., nitrogen and phosphorus) release rates, ingestion rates, and elemental composition of uninfected Daphnia to those infected with a bacterial parasite, P. ramosa. N and P release rates were increased by infection when Daphnia were fed P-poor diets, but we found no effect of infection on the nutrient release of individuals fed P-rich diets. Calculations based on the first law of thermodynamics indicated that infection should increase the nutrient release rates of Daphnia by decreasing nutrient accumulation rates in host tissues. Although we found reduced nutrient accumulation rates in infected Daphnia fed all diets, this reduction did not increase the nutrient release rates of Daphnia fed the P-rich diet because infected Daphnia fed this diet ingested nutrients more slowly than uninfected hosts. Our results thus indicate that parasites can significantly alter the nutrient use of animal consumers, which could affect the availability of nutrients in heavily parasitized environments.

  13. Protection from wintertime rainfall reduces nutrient losses and greenhouse gas emissions during the decomposition of poultry and horse manure-based amendments.

    PubMed

    Maltais-Landry, Gabriel; Neufeld, Katarina; Poon, David; Grant, Nicholas; Nesic, Zoran; Smukler, Sean

    2018-04-01

    Manure-based soil amendments (herein "amendments") are important fertility sources, but differences among amendment types and management can significantly affect their nutrient value and environmental impacts. A 6-month in situ decomposition experiment was conducted to determine how protection from wintertime rainfall affected nutrient losses and greenhouse gas (GHG) emissions in poultry (broiler chicken and turkey) and horse amendments. Changes in total nutrient concentration were measured every 3 months, changes in ammonium (NH 4 + ) and nitrate (NO 3 - ) concentrations every month, and GHG emissions of carbon dioxide (CO 2 ), methane (CH 4 ), and nitrous oxide (N 2 O) every 7-14 days. Poultry amendments maintained higher nutrient concentrations (except for K), higher emissions of CO 2 and N 2 O, and lower CH 4 emissions than horse amendments. Exposing amendments to rainfall increased total N and NH 4 + losses in poultry amendments, P losses in turkey and horse amendments, and K losses and cumulative N 2 O emissions for all amendments. However, it did not affect CO 2 or CH 4 emissions. Overall, rainfall exposure would decrease total N inputs by 37% (horse), 59% (broiler chicken), or 74% (turkey) for a given application rate (wet weight basis) after 6 months of decomposition, with similar losses for NH 4 + (69-96%), P (41-73%), and K (91-97%). This study confirms the benefits of facilities protected from rainfall to reduce nutrient losses and GHG emissions during amendment decomposition. The impact of rainfall protection on nutrient losses and GHG emissions was monitored during the decomposition of broiler chicken, turkey, and horse manure-based soil amendments. Amendments exposed to rainfall had large ammonium and potassium losses, resulting in a 37-74% decrease in N inputs when compared with amendments protected from rainfall. Nitrous oxide emissions were also higher with rainfall exposure, although it had no effect on carbon dioxide and methane emissions. Overall, this work highlights the benefits of rainfall protection during amendment decomposition to reduce nutrient losses and GHG emissions.

  14. Evaluation of Urban Freight Deliveries using Microsimulation and Surrogate Safety Measures

    DOT National Transportation Integrated Search

    2018-02-01

    Freight deliveries on signalized urban streets are known to cause lane blockages during deliveries. When delivery vehicles block lanes of traffic near signalized intersections, the capacity of the intersection is affected. Current practice is for tra...

  15. Generalized Nutrient Taxes Can Increase Consumer Welfare.

    PubMed

    Bishai, David

    2015-11-01

    Certain nutrients can stimulate appetite making them fattening in a way that is not fully conveyed by the calorie content on the label. For rational eaters, this information gap could be corrected by more labeling. As an alternative, this paper proposes a set of positive and negative taxes on the fattening and slimming nutrients in food rather than on the food itself. There are conditions under which this tax plus subsidy system could increase welfare by stopping unwanted weight gain while leaving the final retail price of food unchanged. A nutrient tax system could improve welfare if fattening nutrients, net of their effect on weight, are inferior goods and the fiscal cost of administering the tax is sufficiently low. More data on the price elasticity of demand for nutrients as well as data on how specific nutrients affect satiety and how total calorie intake would be necessary before one could be sure a nutrient tax would work in practice. Copyright © 2014 John Wiley & Sons, Ltd.

  16. On-line process analysis innovation: DiComp (tm) shunting dielectric sensor technology

    NASA Technical Reports Server (NTRS)

    Davis, Craig R.; Waldman, Frank A.

    1993-01-01

    The DiComp Shunting Dielectric Sensor (SDS) is a new patent-pending technology developed under the Small Business Innovation Research Program (SBIR) for NASA's Kennedy Space Center. The incorporation of a shunt electrode into a conventional fringing field dielectric sensor makes the SDS uniquely sensitive to changes in material dielectric properties in the KHz to MHz range which were previously detectable only at GHz measurement frequencies. The initial NASA application of the SDS for Nutrient Delivery Control has demonstrated SDS capabilities for thickness and concentration measurement of Hoagland nutrient solutions. The commercial introduction of DiComp SDS technology for concentration and percent solids measurements in dispersions, emulsions and solutions represents a new technology for process measurements for liquids in a variety of industries.

  17. Sources and Delivery of Nutrients to the Northwestern Gulf of Mexico from Streams in the South-Central United States1

    PubMed Central

    Rebich, Richard A; Houston, Natalie A; Mize, Scott V; Pearson, Daniel K; Ging, Patricia B; Evan Hornig, C

    2011-01-01

    Abstract SPAtially Referenced Regressions On Watershed attributes (SPARROW) models were developed to estimate nutrient inputs [total nitrogen (TN) and total phosphorus (TP)] to the northwestern part of the Gulf of Mexico from streams in the South-Central United States (U.S.). This area included drainages of the Lower Mississippi, Arkansas-White-Red, and Texas-Gulf hydrologic regions. The models were standardized to reflect nutrient sources and stream conditions during 2002. Model predictions of nutrient loads (mass per time) and yields (mass per area per time) generally were greatest in streams in the eastern part of the region and along reaches near the Texas and Louisiana shoreline. The Mississippi River and Atchafalaya River watersheds, which drain nearly two-thirds of the conterminous U.S., delivered the largest nutrient loads to the Gulf of Mexico, as expected. However, the three largest delivered TN yields were from the Trinity River/Galveston Bay, Calcasieu River, and Aransas River watersheds, while the three largest delivered TP yields were from the Calcasieu River, Mermentau River, and Trinity River/Galveston Bay watersheds. Model output indicated that the three largest sources of nitrogen from the region were atmospheric deposition (42%), commercial fertilizer (20%), and livestock manure (unconfined, 17%). The three largest sources of phosphorus were commercial fertilizer (28%), urban runoff (23%), and livestock manure (confined and unconfined, 23%). PMID:22457582

  18. Productivity and river flux variability in response to the PETM on Atlantic margin at Bass River, NJ.

    NASA Astrophysics Data System (ADS)

    Stoll, H.; Shimizu, N.; Savain, R.; Zachos, J.; Ziveri, P.

    2009-04-01

    While the dramatic climate warming of the Paleocene-Eocene Thermal Maximum has been well characterized, changes in the hydrological cycle and the broader biogeochemical feedbacks (weathering, nutrients, productivity) are less well constrained. Here we describe new geochemical results from a coastal section on the midlatitude Atlantic margin of the U.S. at Bass River, NJ. We measured the elemental geochemistry of coccoliths to probe the productivity of these algae in response to the changing nutrient dynamics on the shelf in the time interval preceding and during the PETM. Coccoliths extracted from the siliclastic coastal section at Bass River NJ exhibit exceptionally good preservation and negligible overgrowth compared to typical ocean carbonate-rich sediments. Analysis of individual coccoliths using secondary ion mass spectrometry (SIMS) facilitates reliable trace element measurements in this low-carbonate section. Published sequence stratigraphy and microfossil analysis have revealed several third order sea level cycles in the late Paleocene including a highstand during the PETM. Consequently we extend our paleoproductivity records far below the PETM to characterize this background variability. We recognize a pattern of generally maximum productivity during lowstands and minimal productivity during highstands. Because nutrient concentrations decrease significantly with distance from the coast, highstands reduce productivity by shifting the highest nutrient levels landward, away from the site. This is likely due to greater distance from river sources as well as reduced wave turbulence which mixes nutrients into the photic zone. This general pattern is broken during the PETM, which features high productivity despite a sea level highstand. This anomalous high productivity may reflect enhanced riverine nutrient delivery, and potentially changes in wind strength and mixing intensity. Riverine nutrient delivery could increase with higher precipitation or precipitation seasonality, and/or higher weathering intensity. In support of enhanced riverine flow, seawater d18O ratios, calculated from coccolith d18O and TEX86 temperatures, indicate a strong decrease characteristic of freshening during the PETM. The excellent preservation of coccoliths also enables us to examine interspecific vital effects on coccolith stable isotopes, with minimal diagenetic homogenization. In Bass River coccolith size fractions separated and measured from the same sediments, the vital effects among different size fractions are <0.5 permil in carbon and oxygen isotope ratios, with little change over the PETM, consistent with our earlier results from ODP 690. This contrasts with the large range of stable isotope vital effects in modern cultures and sediment traps, which correlate with cell size and carbonate ion. These results support our hypothesis that the range of interspecific vital effects may have been reduced in the high-CO2 Paleocene ocean when the modern diversity of carbon concentrating mechanisms was not required.

  19. Placentomal differentiation may compensate for maternal nutrient restriction in ewes adapted to harsh range conditions.

    PubMed

    Vonnahme, K A; Hess, B W; Nijland, M J; Nathanielsz, P W; Ford, S P

    2006-12-01

    Maternal nutrient restriction from early to midgestation can lead to fetal growth retardation, with long-term impacts on offspring growth, physiology, and metabolism. We hypothesized that ewes from flocks managed under markedly different environmental conditions and levels of nutrition might differ in their ability to protect their own fetus from a bout of maternal nutrient restriction. We utilized multiparous ewes of similar breeding, age, and parity from 2 flocks managed as 1) ewes adapted to a nomadic existence and year-long, limited nutrition near Baggs, WY (Baggs ewes), and 2) University of Wyoming ewes with a sedentary lifestyle and continuous provision of more than adequate nutrition (UW ewes). Groups of Baggs ewes and UW ewes were fed 50 (nutrient restricted) or 100% (control fed) of National Research Council recommendations from d 28 to 78 of gestation, then necropsied, and fetal and placental data were obtained. Although there was a marked decrease (P < 0.05) in fetal weight and blood glucose concentrations in nutrient-restricted vs. control fed UW ewes, there was no difference in these fetal measurements between nutrient-restricted and control-fed Baggs ewes. Nutrient-restricted and control-fed UW ewes exhibited predominantly type A placentomes on d 78, but there were fewer (P c0.05) type A and greater (P < 0.05) numbers of type B, C, and D placentomes in nutrient-restricted than control-fed Baggs ewes. Placental efficiency (fetal weight/placentomal weight) was reduced (P = 0.04) in d 78 nutrient-restricted UW ewes when compared with control-fed UW ewes. In contrast, nutrient-restricted and control-fed Baggs ewes exhibited similar placental efficiencies on d 78. This is the first report of different placental responses to a nutritional challenge during pregnancy when ewes were selected under different management systems. These data are consistent with the concept that Baggs ewes or their conceptuses, which were adapted to both harsh environments and limited nutrition, initiated conversion of type A placentomes to other placentomal types when subjected to an early to mid-gestational nutrient restriction, whereas this conversion failed to occur in UW ewes. This early placentomal conversion in the Baggs ewes may function to maintain normal nutrient delivery to their developing fetuses during maternal nutrient restriction.

  20. Targeting Urban Watershed Stressor Gradients: Stream Survey Design, Ecological Responses, and Implications of Land Cover Resolution

    EPA Science Inventory

    We conducted a stream survey in the Narragansett Bay Watershed designed to target a gradient of development intensity, and to examine how associated changes in nutrients, carbon, and stressors affect periphyton and macroinvertebrates. Concentrations of nutrients, cations, and ani...

  1. Integrated watershed management for saturation excess generated runoff, erosion and nutrient control

    USDA-ARS?s Scientific Manuscript database

    Understanding the basic hydrology and erosion is vital for effective management and utilization of water resources and soil conservation planning. An important question for judging effectiveness of soil and water conservation practices is whether runoff erosion and nutrient loss is affected by infil...

  2. NUTRIENTS, CANOPY COVER, AND GRAZERS: THEIR EFFECTS ON SUMMER PERIPHYTON IN SMALL MIDWESTERN STREAMS

    EPA Science Inventory

    Numerous studies in artificial streams suggest the relationship between nurients and periphyton biomass (AFDM) and chlorophyll a in streams is affected by ambient light, which is influenced by canopy cover, and by grazer densities. To assess the relationships between nutrients a...

  3. Climate modifies response of non-native and native species richness to nutrient enrichment

    USDA-ARS?s Scientific Manuscript database

    Ecosystem eutrophication often increases domination by non-natives and causes displacement of native taxa. However, variation in environmental conditions may affect the outcome of interactions between native and non-native taxa in environments where nutrient supply is elevated. We examined the int...

  4. Quantitative Models for Ecosystem Assessment in Narragansett Bay: Response to Nutrient Loading and Other Stressors

    EPA Science Inventory

    Multiple drivers, including nutrient loading and climate change, affect the Narragansett Bay ecosystem. Managers are interested in understanding the timing and magnitude of these effects, as well as ecosystem responses to restoration actions, such as the capacity and potential fo...

  5. Avoidance of dairy products: Implications for nutrient adequacy and health

    USDA-ARS?s Scientific Manuscript database

    Dairy products are an important contributor of many essential nutrients often lacking in the typical North American diet, including calcium, potassium, and vitamin D, and limiting dairy intake may adversely affect health. Dairy exclusion diets may exacerbate the risk of osteoporosis and negatively i...

  6. Increases in soil aggregation following phosphorus additions in a tropical premontane forest are not driven by root and arbuscular mycorrhizal fungal abundances

    NASA Astrophysics Data System (ADS)

    Camenzind, Tessa; Papathanasiou, Helena; Foerster, Antje; Dietrich, Karla; Hertel, Dietrich; Homeier, Juergen; Oelmann, Yvonne; Olsson, Pål Axel; Suárez, Juan; Rillig, Matthias

    2015-12-01

    Tropical ecosystems have an important role in global change scenarios, in part because they serve as a large terrestrial carbon pool. Carbon protection is mediated by soil aggregation processes, whereby biotic and abiotic factors influence the formation and stability of aggregates. Nutrient additions may affect soil structure indirectly by simultaneous shifts in biotic factors, mainly roots and fungal hyphae, but also via impacts on abiotic soil properties. Here, we tested the hypothesis that soil aggregation will be affected by nutrient additions primarily via changes in arbuscular mycorrhizal fungal (AMF) hyphae and root length in a pristine tropical forest system. Therefore, the percentage of water-stable macroaggregates (> 250µm) (WSA) and the soil mean weight diameter (MWD) was analyzed, as well as nutrient contents, pH, root length and AMF abundance. Phosphorus additions significantly increased the amount of WSA, which was consistent across two different sampling times. Despite a positive effect of phosphorus additions on extraradical AMF biomass, no relationship between WSA and extra-radical AMF nor roots was revealed by regression analyses, contrary to the proposed hypothesis. These findings emphasize the importance of analyzing soil structure in understudied tropical systems, since it might be affected by increasing nutrient deposition expected in the future.

  7. Statistical modelling of variability in sediment-water nutrient and oxygen fluxes

    NASA Astrophysics Data System (ADS)

    Serpetti, Natalia; Witte, Ursula; Heath, Michael

    2016-06-01

    Organic detritus entering, or produced, in the marine environment is re-mineralised to inorganic nutrient in the seafloor sediments. The flux of dissolved inorganic nutrient between the sediment and overlying water column is a key process in the marine ecosystem, which binds the biogeochemical sub-system to the living food web. These fluxes are potentially affected by a wide range of physical and biological factors and disentangling these is a significant challenge. Here we develop a set of General Additive Models (GAM) of nitrate, nitrite, ammonia, phosphate, silicate and oxygen fluxes, based on a year-long campaign of field measurements off the north-east coast of Scotland. We show that sediment grain size, turbidity due to sediment re-suspension, temperature, and biogenic matter content were the key factors affecting oxygen consumption, ammonia and silicate fluxes. However, phosphate fluxes were only related to suspended sediment concentrations, whilst nitrate fluxes showed no clear relationship to any of the expected drivers of change, probably due to the effects of denitrification. Our analyses show that the stoichiometry of nutrient regeneration in the ecosystem is not necessarily constant and may be affected by combinations of processes. We anticipate that our statistical modelling results will form the basis for testing the functionality of process-based mathematical models of whole-sediment biogeochemistry.

  8. The afterlife of interspecific indirect genetic effects: genotype interactions alter litter quality with consequences for decomposition and nutrient dynamics.

    PubMed

    Genung, Mark A; Bailey, Joseph K; Schweitzer, Jennifer A

    2013-01-01

    Aboveground-belowground linkages are recognized as divers of community dynamics and ecosystem processes, but the impacts of plant-neighbor interactions on these linkages are virtually unknown. Plant-neighbor interactions are a type of interspecific indirect genetic effect (IIGE) if the focal plant's phenotype is altered by the expression of genes in a neighboring heterospecific plant, and IIGEs could persist after plant senescence to affect ecosystem processes. This perspective can provide insight into how plant-neighbor interactions affect evolution, as IIGEs are capable of altering species interactions and community composition over time. Utilizing genotypes of Solidago altissima and Solidago gigantea, we experimentally tested whether IIGEs that had affected living focal plants would affect litter decomposition rate, as well as nitrogen (N) and phosphorous (P) dynamics after the focal plant senesced. We found that species interactions affected N release and genotype interactions affected P immobilization. From a previous study we knew that neighbor genotype influenced patterns of biomass allocation for focal plants. Here we extend those previous results to show that these changes in biomass allocation altered litter quality, that then altered rates of decomposition and nutrient cycling. Our results provide insights into above- and belowground linkages by showing that, through their effects on plant litter quality (e.g., litter lignin:N), IIGEs can have afterlife effects, tying plant-neighbor interactions to ecosystem processes. This holistic approach advances our understanding of decomposition and nutrient cycling by showing that evolutionary processes (i.e., IIGEs) can influence ecosystem functioning after plant senescence. Because plant traits are determined by the combined effects of genetic and environmental influences, and because these traits are known to affect decomposition and nutrient cycling, we suggest that ecosystem processes can be described as gene-less products of genetic interactions among the species comprising ecological communities.

  9. The Afterlife of Interspecific Indirect Genetic Effects: Genotype Interactions Alter Litter Quality with Consequences for Decomposition and Nutrient Dynamics

    PubMed Central

    Genung, Mark A.; Bailey, Joseph K.; Schweitzer, Jennifer A.

    2013-01-01

    Aboveground-belowground linkages are recognized as divers of community dynamics and ecosystem processes, but the impacts of plant-neighbor interactions on these linkages are virtually unknown. Plant-neighbor interactions are a type of interspecific indirect genetic effect (IIGE) if the focal plant’s phenotype is altered by the expression of genes in a neighboring heterospecific plant, and IIGEs could persist after plant senescence to affect ecosystem processes. This perspective can provide insight into how plant-neighbor interactions affect evolution, as IIGEs are capable of altering species interactions and community composition over time. Utilizing genotypes of Solidago altissima and Solidago gigantea, we experimentally tested whether IIGEs that had affected living focal plants would affect litter decomposition rate, as well as nitrogen (N) and phosphorous (P) dynamics after the focal plant senesced. We found that species interactions affected N release and genotype interactions affected P immobilization. From a previous study we knew that neighbor genotype influenced patterns of biomass allocation for focal plants. Here we extend those previous results to show that these changes in biomass allocation altered litter quality, that then altered rates of decomposition and nutrient cycling. Our results provide insights into above- and belowground linkages by showing that, through their effects on plant litter quality (e.g., litter lignin:N), IIGEs can have afterlife effects, tying plant-neighbor interactions to ecosystem processes. This holistic approach advances our understanding of decomposition and nutrient cycling by showing that evolutionary processes (i.e., IIGEs) can influence ecosystem functioning after plant senescence. Because plant traits are determined by the combined effects of genetic and environmental influences, and because these traits are known to affect decomposition and nutrient cycling, we suggest that ecosystem processes can be described as gene-less products of genetic interactions among the species comprising ecological communities. PMID:23349735

  10. Urbanization effects on leaf litter decomposition, foliar nutrient dynamics and aboveground net primary productivity in the subtropics

    Treesearch

    Heather A. Enloe; B. Graeme Lockaby; Wayne C. Zipperer; Greg L. Somers

    2015-01-01

    Urbanization can alter nutrient cycling. This research evaluated how urbanization affected nutrient dynamics in the subtropics. We established 17–0.04 ha plots in five different land cover types—slash pine (Pinus elliottii) plantations (n=3), rural natural pine forests (n= 3), rural natural oak forests (n=4), urban pine forests (n=3) and urban oak forests (n=4) in the...

  11. The Effectiveness of Distance Education, Using Blended Method of Delivery for Limited-Resource Audiences in the Nutrition Education Program

    ERIC Educational Resources Information Center

    Campbell, Casey; Koszewski, Wanda M.; Behrends, Donnia

    2013-01-01

    The study reported here sought to determine if the use of distance education lessons for teaching limited resource participants in a nutrition education program (NEP) is as effective as face-to-face methodology. One hundred and six participants were in the experimental group. Data was gathered at entry and examined behavior change, nutrient intake…

  12. Teaching children about good health? Halo effects in child-directed advertisements for unhealthy food.

    PubMed

    Harris, J L; Haraghey, K S; Lodolce, M; Semenza, N L

    2018-04-01

    Food companies often use healthy lifestyle messages in child-directed advertising, raising public health concerns about health halo effects for nutrient-poor food/drinks. Examine effects of health messages promoting nutrient-poor foods in child-directed advertising. Randomized controlled experiment (N = 138). Children (7-11 years) viewed three child-friendly commercials in one of three conditions: (1) health halo (unfamiliar nutrient-poor food/drink ads with healthy messages); (2) nutrient-poor food/drink ads with other messages and (3) healthy food/drink ads. They rated the commercials and advertised products, provided attitudes about exercise and nutrition and consumed and rated healthy and unhealthy snack foods. Children in the health halo condition rated the advertised nutrient-poor products as significantly healthier compared with children in other conditions (p = .003), but the other commercials did not affect children's attitudes about other advertised products (p's > .50). Child age, gender or TV viewing habits did not significantly predict their ratings (p's > .18). There was no evidence that healthy lifestyle messages and/or healthy food commercials improved children's attitudes about nutrition, exercise or healthy snack consumption. Promoting healthy lifestyle messages in child-directed commercials for nutrient-poor food/drinks likely benefits brands by increasing products' perceived healthfulness, but these ads are unlikely to positively affect children's attitudes about health and nutrition. © 2017 World Obesity Federation.

  13. Effects of Prey Macronutrient Content on Body Composition and Nutrient Intake in a Web-Building Spider

    PubMed Central

    Hawley, Jesse; Simpson, Stephen J.; Wilder, Shawn M.

    2014-01-01

    The nutritional composition of diets can vary widely in nature and have large effects on the growth, reproduction and survival of animals. Many animals, especially herbivores, will tightly regulate the nutritional composition of their body, which has been referred to as nutritional homeostasis. We tested how experimental manipulation of the lipid and protein content of live prey affected the nutrient reserves and subsequent diet regulation of web-building spiders, Argiope keyserlingi. Live locusts were injected with experimental solutions containing specific amounts of lipid and protein and then fed to spiders. The nutrient composition of the spiders' bodies was directly related to the nutrient composition of the prey on which they fed. We then conducted an experiment where spiders were fed either high lipid or high protein prey and subsequently provided with two large unmanipulated locusts. Prior diet did not affect the amount or ratio of lipid and protein ingested by spiders when feeding on unmanipulated prey. Argiope keyserlingi were flexible in the storage of lipid and protein in their bodies and did not bias their extraction of nutrients from prey to compensate for previously biased diets. Some carnivores, especially those that experience frequent food limitation, may be less likely to strictly regulate their body composition than herbivores because food limitation may encourage opportunistic ingestion and assimilation of nutrients. PMID:24911958

  14. Concurrent Effects of Sediment Accretion and Nutrient Availability on the Clonal Growth Strategy of Carex brevicuspis-A Wetland Sedge That Produces Both Spreading and Clumping Ramets

    PubMed Central

    Chen, Xinsheng; Liao, Yulin; Xie, Yonghong; Li, Feng; Deng, Zhengmiao; Hou, Zhiyong; Wu, Chao

    2017-01-01

    Clonal plants producing both clumping and spreading ramets can adjust their growth forms in response to resource heterogeneity or environmental stress. They might produce clumping ramets to retain favorable patches, or produce spreading ramets to escape from stress-affected patches. This study aimed to investigate the rarely reported concurrent effects of sediment accretion and nutrient enrichment, which often occur simultaneously in lacustrine wetlands, on the vegetative propagation and clonal growth forms of Carex brevicuspis C.B. Clarke by conducting a factorial experiment of sediment burial and nutrient addition. Biomass accumulation, new ramet and rhizome numbers, and ramet length of C. brevicuspis were not affected at moderate burial, but were significantly lower after deep burial. Similarly, nutrient enrichment increased the growth and vegetative propagation of C. brevicuspis up to moderate sediment burial, but not after deep burial. Sediment accretion increased the proportion of spreading ramets produced by C. brevicuspis, whereas nutrient addition had no effect on the clonal growth forms. Our results indicated that the plasticity of clonal growth forms is an effective strategy used by plants to acclimate to moderate sediment accretion. Nutrient enrichment did not influence the clonal growth forms of C. brevicuspis and could not facilitate its acclimation to heavy sedimentation condition. PMID:29021805

  15. Comparison of two methods for estimating discharge and nutrient loads from Tidally affected reaches of the Myakka and Peace Rivers, West-Central Florida

    USGS Publications Warehouse

    Levesque, V.A.; Hammett, K.M.

    1997-01-01

    The Myakka and Peace River Basins constitute more than 60 percent of the total inflow area and contribute more than half the total tributary inflow to the Charlotte Harbor estuarine system. Water discharge and nutrient enrichment have been identified as significant concerns in the estuary, and consequently, it is important to accurately estimate the magnitude of discharges and nutrient loads transported by inflows from both rivers. Two methods for estimating discharge and nutrient loads from tidally affected reaches of the Myakka and Peace Rivers were compared. The first method was a tidal-estimation method, in which discharge and nutrient loads were estimated based on stage, water-velocity, discharge, and water-quality data collected near the mouths of the rivers. The second method was a traditional basin-ratio method in which discharge and nutrient loads at the mouths were estimated from discharge and loads measured at upstream stations. Stage and water-velocity data were collected near the river mouths by submersible instruments, deployed in situ, and discharge measurements were made with an acoustic Doppler current profiler. The data collected near the mouths of the Myakka River and Peace River were filtered, using a low-pass filter, to remove daily mixed-tide effects with periods less than about 2 days. The filtered data from near the river mouths were used to calculate daily mean discharge and nutrient loads. These tidal-estimation-method values were then compared to the basin-ratio-method values. Four separate 30-day periods of differing streamflow conditions were chosen for monitoring and comparison. Discharge and nutrient load estimates computed from the tidal-estimation and basin-ratio methods were most similar during high-flow periods. However, during high flow, the values computed from the tidal-estimation method for the Myakka and Peace Rivers were consistently lower than the values computed from the basin-ratio method. There were substantial differences between discharges and nutrient loads computed from the tidal-estimation and basin-ratio methods during low-flow periods. Furthermore, the differences between the methods were not consistent. Discharges and nutrient loads computed from the tidal-estimation method for the Myakka River were higher than those computed from the basin-ratio method, whereas discharges and nutrients loads computed by the tidal-estimation method for the Peace River were not only lower than those computed from the basin-ratio method, but they actually reflected a negative, or upstream, net movement. Short-term tidal measurement results should be used with caution, because antecedent conditions can influence the discharge and nutrient loads. Continuous tidal data collected over a 1- or 2-year period would be necessary to more accurately estimate the tidally affected discharge and nutrient loads for the Myakka and Peace River Basins.

  16. Effects of predation and nutrient enrichment on the success and microbiome of a foundational coral.

    PubMed

    Shaver, Elizabeth C; Shantz, Andrew A; McMinds, Ryan; Burkepile, Deron E; Vega Thurber, Rebecca L; Silliman, Brian R

    2017-03-01

    By inflicting damage to prey tissues, consumer species may increase stress in prey hosts and reduce overall fitness (i.e., primary effects, such as growth or reproduction) or cause secondary effects by affecting prey interactions with other species such as microbes. However, little is known about how abiotic conditions affect the outcomes of these biotic interactions. In coral reef communities, both nutrient enrichment and predation have been linked to reduced fitness and disease facilitation in corals, yet no study to date has tested their combined effects on corals or their associated microbial communities (i.e., microbiomes). Here, we assess the effects of grazing by a prevalent coral predator (the short coral snail, Coralliophila abbreviata) and nutrient enrichment on staghorn coral, Acropora cervicornis, and its microbiomes using a factorial experiment and high-throughput DNA sequencing. We found that predation, but not nutrients, significantly reduced coral growth and increased mortality, tissue loss, and turf algae colonization. Partial predation and nutrient enrichment both independently altered coral microbiomes such that one bacterial genus came to dominate the microbial community. Nutrient-enriched corals were associated with significant increases in Rickettsia-like organisms, which are currently one of several microbial groups being investigated as a disease agent in this coral species. However, we found no effects of nutrient enrichment on coral health, disease, or their predators. This research suggests that in the several months following coral transplantation (i.e., restoration) or disturbance (i.e., recovery), Caribbean acroporid corals appear to be highly susceptible to negative effects caused by predators, but not or not yet susceptible to nutrient enrichment despite changes to their microbial communities. © 2016 by the Ecological Society of America.

  17. Functioning of a Shallow-Water Sediment System during Experimental Warming and Nutrient Enrichment

    PubMed Central

    Alsterberg, Christian; Sundbäck, Kristina; Hulth, Stefan

    2012-01-01

    Effects of warming and nutrient enrichment on intact unvegetated shallow-water sediment were investigated for 5 weeks in the autumn under simulated natural field conditions, with a main focus on trophic state and benthic nitrogen cycling. In a flow-through system, sediment was exposed to either seawater at ambient temperature or seawater heated 4°C above ambient, with either natural or nutrient enriched water. Sediment–water fluxes of oxygen and inorganic nutrients, nitrogen mineralization, and denitrification were measured. Warming resulted in an earlier shift to net heterotrophy due to increased community respiration; primary production was not affected by temperature but (slightly) by nutrient enrichment. The heterotrophic state was, however, not further strengthened by warming, but was rather weakened, probably because increased mineralization induced a shortage of labile organic matter. Climate-related warming of seawater during autumn could therefore, in contrast to previous predictions, induce shorter but more intensive heterotrophic periods in shallow-water sediments, followed by longer autotrophic periods. Increased nitrogen mineralization and subsequent effluxes of ammonium during warming suggested a preferential response of organisms driving nitrogen mineralization when compared to sinks of ammonium such as nitrification and algal assimilation. Warming and nutrient enrichment resulted in non-additive effects on nitrogen mineralization and denitrification (synergism), as well as on benthic fluxes of phosphate (antagonism). The mode of interaction appears to be related to the trophic level of the organisms that are the main drivers of the affected processes. Despite the weak response of benthic microalgae to both warming and nutrient enrichment, the assimilation of nitrogen by microalgae was similar in magnitude to rates of nitrogen mineralization. This implies a sustained filter function and retention capacity of nutrients by the sediment. PMID:23240032

  18. Proteomic characterization of intermediate and advanced glycation end-products in commercial milk samples.

    PubMed

    Renzone, Giovanni; Arena, Simona; Scaloni, Andrea

    2015-03-18

    The Maillard reaction consists of a number of chemical processes affecting the structure of the proteins present in foods. We previously accomplished the proteomic characterization of the lactosylation targets in commercial milk samples. Although characterizing the early modification derivatives, this analysis did not describe the corresponding advanced glycation end-products (AGEs), which may be formed from the further oxidation of former ones or by reaction of oxidized sugars with proteins, when high temperatures are exploited. To fill this gap, we have used combined proteomic procedures for the systematic characterization of the lactosylated and AGE-containing proteins from the soluble and milk fat globule membrane fraction of various milk products. Besides to confirm all lactulosyl-lysines described previously, 40 novel lactosylation sites were identified. More importantly, 308 additional intermediate and advanced glyco-oxidation derivatives (including cross-linking adducts) were characterized in 31 proteins, providing the widest qualitative inventory of modified species ascertained in commercial milk samples so far. Amadori adducts with glucose/galactose, their dehydration products, carboxymethyllysine and glyoxal-, 3-deoxyglucosone/3-deoxygalactosone- and 3-deoxylactosone-derived dihydroxyimidazolines and/or hemiaminals were the most frequent derivatives observed. Depending on thermal treatment, a variable number of modification sites was identified within each protein; their number increased with harder food processing conditions. Among the modified proteins, species involved in assisting the delivery of nutrients, defense response against pathogens and cellular proliferation/differentiation were highly affected by AGE formation. This may lead to a progressive decrease of the milk nutritional value, as it reduces the protein functional properties, abates the bioavailability of the essential amino acids and eventually affects food digestibility. These aspects are of particular importance in products intended for infant diet, such as milk powders and infant formulas. We used combined shotgun proteomic procedures for the systematic characterization of intermediate and advanced glycoxidation protein products in various raw and commercial milk samples. Several hundreds of modified species were characterized as deriving from 31 milk proteins, providing the widest qualitative inventory of assigned components in this fluid. Amadori adducts with glucose/galactose, their dehydration products, carboxymethyl-lysine, and glyoxal-, 3-deoxyglucosone/3-deoxygalactosone- and 3-deoxylactosone-derived dihydroxyimidazolines and/or hemiaminals were the most frequent derivatives observed. Proteins involved in nutrient delivery, defense response against pathogens and cellular proliferation/differentiation were highly subjected to intermediate and advanced glyco-oxidation modification. This may lead to a progressive decrease of the milk nutritional value, as it reduces the protein functional properties, diminishes the bioavailability of the essential amino acids, eventually affects food digestibility and determines a potential increase of specific allergens. These information are important points of interest to connect the extent of the Maillard reaction present in different commercial samples with the potential nutritional aspects mentioned above. These themes have to be fully evaluated in a next future for a complete estimation of the nutritional and toxicological properties of the dairy products deriving from severe heat processing. Copyright © 2015 Elsevier B.V. All rights reserved.

  19. Fluorescence spectroscopic analysis of organic matter fractions: the current status and a tutorial case study

    USDA-ARS?s Scientific Manuscript database

    Incorporation of animal manures into soils is a key nutrient management strategy for sustainable agricultural systems by supplying plant nutrients and maintaining soil quality. Dissolved organic matter (DOM) released from manures affects many soil chemical processes due to its reactivity with soil ...

  20. Relationships Among Watershed Condition, Nutrients, and Algae in New England Streams Affected by Urbanization

    EPA Science Inventory

    We examined algal metrics as indicators of altered watershed land cover and nutrients to inform their potential use in monitoring programs. Multiple regression models, in which impervious cover explained the most variation, indicated concentrations <0.202 mg/l NO3 and <0.015 mg/l...

  1. ABSORPTION OF NUTRIENTS AND PLANT GROWTH IN RELATION TO HYDROGEN ION CONCENTRATION

    PubMed Central

    Arrhenius, Olof

    1922-01-01

    The absorption of nutrients depends to a large extent on the reaction of the substrate. At maximal growth the intake of salt is at minimum. Different ions are very differently affected. The intake of water is independent of the absorption of salts. PMID:19871980

  2. Elevated enzyme activities in soils under the invasive nitrogen-fixing tree Falcataria moluccana

    Treesearch

    Steven D. Allison; Caroline Nielsen; R. Flint Hughes

    2006-01-01

    Like other N-fixing invasive species in Hawaii, Falcataria moluccana dramatically alters forest structure, litterfall quality and quantity, and nutrient dynamics. We hypothesized that these biogeochemical changes would also affect the soil microbial community and the extracellular enzymes responsible for carbon and nutrient mineralization. Across...

  3. Nutrient fortification of human donor milk affects intestinal function and protein metabolism in preterm pigs

    USDA-ARS?s Scientific Manuscript database

    Nutrient fortification of human milk is often required to secure adequate growth and organ development for very preterm infants. There is concern that formula-based fortifiers (FFs) induce intestinal dysfunction, feeding intolerance, and necrotizing enterocolitis (NEC). Bovine colostrum (BC) may be ...

  4. DEVELOPMENT OF NITROGEN LOADING-RESPONSE RELATIONSHIPS FOR ESTUARINE WATERS USING AN EMPIRICAL COMPARATIVE SYSTEMS APPROACH

    EPA Science Inventory

    There is growing evidence that human activities have dramatically changed the amounts, distribution, and movement of major nutrient elements (nitrogen-N and phosphorus-P) in the landscape and have increased nutrient loading to receiving waters. Some of these changes affect use o...

  5. Influence of catchment land cover on stoichiometry and stable isotope compositions of basal resources and macroinvertebrate consumers in headwater streams

    EPA Science Inventory

    Anthropogenic land use affects aquatic landscapes. For example, landscape-level conversion to urban or agricultural land can heavily influence nutrient cycles in headwater streams via increased nutrient loading and altered hydrologic patterns. Recent studies in headwater streams ...

  6. Quantitative Models Describing Past and Current Nutrient Fluxes and Associated Ecosystem Level Responses in the Narragansett Bay Ecosystem

    EPA Science Inventory

    Multiple drivers, including nutrient loading and climate change, affect the Narragansett Bay ecosystem in Rhode Island/Massachusetts, USA. Managers are interested in understanding the timing and magnitude of these effects, and ecosystem responses to restoration actions. To provid...

  7. Reconnaissance investigation of water quality, bottom sediment, and biota associated with irrigation drainage in and near Humboldt Wildlife Management Area, Churchill and Pershing Counties, Nevada, 1990-91

    USGS Publications Warehouse

    Seiler, R.L.; Ekechukwu, G.A.; Hallock, R.J.

    1993-01-01

    A reconnaissance investigation was begun in 1990 to determine whether the quality of irrigation drainage in and near the Humboldt Wildlife Management Area, Nevada, has caused or has the potential to cause harmful effects on human health, fish, and wildlife or to impair beneficial uses of water. Samples of surface and ground water, bottom sediment, and biota collected from sites upstream and downstream from the Lovelock agricultural area were analyzed for potentially toxic trace elements. Also analyzed were radioactive substances, major dissolved constitu- ents, and nutrients in water, as well as pesticide residues in bottom sediment and biota. In samples from areas affected by irrigation drainage, the following constituents equaled or exceeded baseline concentrations or recommended standards for protection of aquatic life or propagation of wildlife--in water: arsenic, boron, dissolved solids, mercury, molybdenum, selenium, sodium, and un-ionized ammonia; in bottom sediment; arsenic and uranium; and in biota; arsenic, boron, and selenium. Selenium appears to be biomagnified in the Humboldt Sink wetlands. Biological effects observed during the reconnaissance included reduced insect diversity in sites receiving irrigation drainage and acute toxicity of drain water and sediment to test organisms. The current drought and upstream consumption of water for irrigation have reduced water deliveries to the wetlands and caused habitat degradation at Humboldt Wildlife Management Area. During this investigation. Humboldt and Toulon Lakes evaporated to dryness because of the reduced water deliveries.

  8. Insulin regulates its own delivery to skeletal muscle by feed-forward actions on the vasculature

    PubMed Central

    Wang, Hong; Upchurch, Charles T.; Liu, Zhenqi

    2011-01-01

    Insulin, at physiological concentrations, regulates the volume of microvasculature perfused within skeletal and cardiac muscle. It can also, by relaxing the larger resistance vessels, increase total muscle blood flow. Both of these effects require endothelial cell nitric oxide generation and smooth muscle cell relaxation, and each could increase delivery of insulin and nutrients to muscle. The capillary microvasculature possesses the greatest endothelial surface area of the body. Yet, whether insulin acts on the capillary endothelial cell is not known. Here, we review insulin's actions at each of three levels of the arterial vasculature as well as recent data suggesting that insulin can regulate a vesicular transport system within the endothelial cell. This latter action, if it occurs at the capillary level, could enhance insulin delivery to muscle interstitium and thereby complement insulin's actions on arteriolar endothelium to increase insulin delivery. We also review work that suggests that this action of insulin on vesicle transport depends on endothelial cell nitric oxide generation and that insulin's ability to regulate this vesicular transport system is impaired by inflammatory cytokines that provoke insulin resistance. PMID:21610226

  9. Invasion by the Alien Tree Prunus serotina Alters Ecosystem Functions in a Temperate Deciduous Forest

    PubMed Central

    Aerts, Raf; Ewald, Michael; Nicolas, Manuel; Piat, Jérôme; Skowronek, Sandra; Lenoir, Jonathan; Hattab, Tarek; Garzón-López, Carol X.; Feilhauer, Hannes; Schmidtlein, Sebastian; Rocchini, Duccio; Decocq, Guillaume; Somers, Ben; Van De Kerchove, Ruben; Denef, Karolien; Honnay, Olivier

    2017-01-01

    Alien invasive species can affect large areas, often with wide-ranging impacts on ecosystem structure, function, and services. Prunus serotina is a widespread invader of European temperate forests, where it tends to form homogeneous stands and limits recruitment of indigenous trees. We hypotesized that invasion by P. serotina would be reflected in the nutrient contents of the native species' leaves and in the respiration of invaded plots as efficient resource uptake and changes in nutrient cycling by P. serotina probably underly its aggressive invasiveness. We combined data from 48 field plots in the forest of Compiègne, France, and data from an experiment using 96 microcosms derived from those field plots. We used general linear models to separate effects of invasion by P. serotina on heterotrophic soil and litter respiration rates and on canopy foliar nutrient content from effects of soil chemical properties, litter quantity, litter species composition, and tree species composition. In invaded stands, average respiration rates were 5.6% higher for soil (without litter) and 32% higher for soil and litter combined. Compared to indigenous tree species, P. serotina exhibited higher foliar N (+24.0%), foliar P (+50.7%), and lower foliar C:N (−22.4%) and N:P (−10.1%) ratios. P. serotina affected foliar nutrient contents of co-occuring indigenous tree species leading to decreased foliar N (−8.7 %) and increased C:N ratio (+9.5%) in Fagus sylvatica, decreased foliar N:P ratio in Carpinus betulus (−13.5%) and F. sylvatica (−11.8%), and increased foliar P in Pinus sylvestris (+12.3%) in invaded vs. uninvaded stands. Our results suggest that P. serotina is changing nitrogen, phosphorus, and carbon cycles to its own advantage, hereby increasing carbon turnover via labile litter, affecting the relative nutrient contents in the overstory leaves, and potentially altering the photosynthetic capacity of the long-lived indigenous broadleaved species. Uncontrolled invasion of European temperate forests by P. serotina may affect the climate change mitigation potential of these forests in the long term, through additive effects on local nutrient cycles. PMID:28261238

  10. Periconceptional nutrient intakes and risks of neural tube defects in California.

    PubMed

    Carmichael, Suzan L; Yang, Wei; Shaw, Gary M

    2010-08-01

    This study investigated the association of neural tube defects (NTDs) with maternal periconceptional intake of folic acid-containing supplements and dietary nutrients, including folate, among deliveries that occurred after folic acid fortification in selected California counties. The population-based case-control study included fetuses and live born infants with spina bifida (189) or anencephaly (141) and 625 nonmalformed, live born controls delivered from 1999 to 2003. Mothers reported supplement use during telephone interviews, which included a 107-item food frequency questionnaire. For dietary nutrients, intakes <25th, 25th to <75th (reference), and > or =75th percentile were compared, based on control distributions. After adjustment for potential confounders, any versus no supplement intake resulted in ORs of 0.8 (95% CI, 0.5-1.3) for anencephaly and 0.8 (95% CI, 0.6-1.2) for spina bifida. After stratification by maternal intake of vitamin supplements, most factors in the glycemic pathway were not associated with either NTD, with the exception of low levels of fructose and glucose that were significantly associated with anencephaly. Some nutrients that contribute to one-carbon metabolism showed lowered risks (folate, riboflavin, vitamins B(6) and B(12)); others did not (choline, methionine, zinc). Antioxidant nutrients tended to be associated with lowered risks (vitamins C, E, A, beta-carotene, lutein). Mothers' intake of vitamin supplements was modestly if at all associated with a lowered risk of NTDs. Dietary intake of several nutrients contributing to one-carbon metabolism and oxidative stress were associated with reduced NTD risk.

  11. The dietary composition of women who delivered preterm and full-term infants.

    PubMed

    Waksmańska, Wioletta; Bobiński, Rafał; Ulman-Włodarz, Izabela; Pielesz, Anna; Mikulska, Monika

    2017-06-01

    Literature data show that excess and primary deficiency in particular nutrients, vitamins and minerals may lead to pre-eclampsia, gestational diabetes, hypertension and neural tube defects in the foetus. The aim of the study was to determine differences in average daily consumption of selected nutrients during pregnancy in women who did not supplement their diet and to evaluate the influence of dietary habits on the occurrence of pre-term delivery and hypertension in pregnant women. Information on the course of pregnancy and the newborn's health status at birth was derived from the Charter of Pregnancy and documents recorded by the hospital. Women's eating habits and dietary composition were analyzed on the basis of a dietary questionnaire. The sample group was divided into four groups: women who delivered neonates appropriate for gestational age (AGA), women with gestosis who delivered AGA neonates by means of caesarean sections, women who delivered pre-term neonates (PTB) and women with gestosis who delivered PTB by means of caesarean sections. In the case of women with vaginal delivery at term the average intake of iodine was always higher than in other groups. Analysis of average daily intake of folates revealed a higher intake in the group of women who gave birth to full-term neonates with proper neonatal weight in comparison with the groups of women with pre-term delivery. P≤0.05. Statistically significant differences in average daily intake of folates, iodine, retinol, magnesium and iron were observed between the group of women with vaginal delivery at term and the groups of women with diagnosed hypertension who delivered preterm. Correlation was demonstrated between average daily intake of iodine and vitamin D and the occurrence of arterial hypertension. Supplementation of the diet of women in the preconception and prenatal period with minerals and vitamins should be considered. Copyright © 2017 Elsevier Inc. All rights reserved.

  12. Effects of herbivory, nutrients, and reef protection on algal proliferation and coral growth on a tropical reef.

    PubMed

    Rasher, Douglas B; Engel, Sebastian; Bonito, Victor; Fraser, Gareth J; Montoya, Joseph P; Hay, Mark E

    2012-05-01

    Maintaining coral reef resilience against increasing anthropogenic disturbance is critical for effective reef management. Resilience is partially determined by how processes, such as herbivory and nutrient supply, affect coral recovery versus macroalgal proliferation following disturbances. However, the relative effects of herbivory versus nutrient enrichment on algal proliferation remain debated. Here, we manipulated herbivory and nutrients on a coral-dominated reef protected from fishing, and on an adjacent macroalgal-dominated reef subject to fishing and riverine discharge, over 152 days. On both reefs, herbivore exclusion increased total and upright macroalgal cover by 9-46 times, upright macroalgal biomass by 23-84 times, and cyanobacteria cover by 0-27 times, but decreased cover of encrusting coralline algae by 46-100% and short turf algae by 14-39%. In contrast, nutrient enrichment had no effect on algal proliferation, but suppressed cover of total macroalgae (by 33-42%) and cyanobacteria (by 71% on the protected reef) when herbivores were excluded. Herbivore exclusion, but not nutrient enrichment, also increased sediment accumulation, suggesting a strong link between herbivory, macroalgal growth, and sediment retention. Growth rates of the corals Porites cylindrica and Acropora millepora were 30-35% greater on the protected versus fished reef, but nutrient and herbivore manipulations within a site did not affect coral growth. Cumulatively, these data suggest that herbivory rather than eutrophication plays the dominant role in mediating macroalgal proliferation, that macroalgae trap sediments that may further suppress herbivory and enhance macroalgal dominance, and that corals are relatively resistant to damage from some macroalgae but are significantly impacted by ambient reef condition.

  13. Fluid-Structure Interaction in Continuum Models of Bacterial Biofilms

    NASA Astrophysics Data System (ADS)

    Hicks, Jared A.

    Bacterial biofilms are aggregates of cells that adhere to nearly any solid-fluid interface. While many have harmful effects, such as industrial damage and nosocomial infections, certain biofilm species are now generating renewable energy as the fundamental components of Microbial Fuel Cells (MFCs). In an MFC, bacteria consume organic waste and, as they respire, produce free electrons. To do so efficiently, the bacteria must operate at peak metabolic activity, and so require an ample supply of nutrients. But existing MFC systems face several nutrient delivery problems, including clogging and downstream depletion. Ameliorating these problems will require a better understanding of the interplay between structural development and the surrounding fluid flow. In addition to delivering nutrients that affect biofilm growth, the fluid also exerts stresses that cause erosion, detachment, and deformation. These structural changes, in turn, affect the flow and alter the nutrient distribution. To account for this feedback effect, I have developed a continuum model that couples the growth and deformation processes. My model augments an existing growth model with evolution equations derived from Morphoelasticity Theory, by showing that the growth tensor can be directly related to the biofilm velocity potential. This result helps overcome one of the major practical limitations of Morphoelasticity--there is no physical framework for specifying the growth tensor. Through further analysis of the growth tensor, I define the related adjugate and anisotropic growth tensors, which can be more meaningful measures of growth for some models. Under the assumption of small strain, I show that there exists a small correction to the biofilm growth velocity (the accommodation velocity) that represents the effect of the elastic response on the evolution of the biofilm shape. I derive a solvability condition for the accommodation velocity, and show that it leads to a novel evolution equation for stress and strain in the biofilm, which couples the growth and deformation processes. Furthermore, I show that the introduction of a vorticity allows the accommodation velocity to be described by a system of Poisson equations, and that this vorticity arises naturally from Morphoelasticity theory and is related to the velocity solvability condition. I apply the modeling approach to a one-dimensional biofilm, and show that (a) the coupled growth process affects the evolution of the biofilm shape as expected, and (b) a non-coupled approach to biofilm strain introduces an error that grows over time. Numerical analysis of the one-dimensional strain evolution equation leads to several insights that inform the development of numerical methods for the two-dimensional case, including a split-step approach that reduces the fifth-order PDE to an advection equation for strain and a biharmonic equation for stress. Finally, I discuss some useful numerical methods for the simulation of elastic biofilm growth, particularly the discretization of the strain evolution equation(s). My overall approach is to track the evolving biofilm surface using a combination of the level-set method coupled with the eXtended Finite Element Method (XFEM). The major result is a novel mixed-XFEM discretization of the clamped-plate biharmonic equation, which I show to be first-order accurate for the trace of the solution on the interface.

  14. Incorporating uncertainty into the ranking of SPARROW model nutrient yields from Mississippi/Atchafalaya River basin watersheds

    USGS Publications Warehouse

    Robertson, Dale M.; Schwarz, Gregory E.; Saad, David A.; Alexander, Richard B.

    2009-01-01

    Excessive loads of nutrients transported by tributary rivers have been linked to hypoxia in the Gulf of Mexico. Management efforts to reduce the hypoxic zone in the Gulf of Mexico and improve the water quality of rivers and streams could benefit from targeting nutrient reductions toward watersheds with the highest nutrient yields delivered to sensitive downstream waters. One challenge is that most conventional watershed modeling approaches (e.g., mechanistic models) used in these management decisions do not consider uncertainties in the predictions of nutrient yields and their downstream delivery. The increasing use of parameter estimation procedures to statistically estimate model coefficients, however, allows uncertainties in these predictions to be reliably estimated. Here, we use a robust bootstrapping procedure applied to the results of a previous application of the hybrid statistical/mechanistic watershed model SPARROW (Spatially Referenced Regression On Watershed attributes) to develop a statistically reliable method for identifying “high priority” areas for management, based on a probabilistic ranking of delivered nutrient yields from watersheds throughout a basin. The method is designed to be used by managers to prioritize watersheds where additional stream monitoring and evaluations of nutrient-reduction strategies could be undertaken. Our ranking procedure incorporates information on the confidence intervals of model predictions and the corresponding watershed rankings of the delivered nutrient yields. From this quantified uncertainty, we estimate the probability that individual watersheds are among a collection of watersheds that have the highest delivered nutrient yields. We illustrate the application of the procedure to 818 eight-digit Hydrologic Unit Code watersheds in the Mississippi/Atchafalaya River basin by identifying 150 watersheds having the highest delivered nutrient yields to the Gulf of Mexico. Highest delivered yields were from watersheds in the Central Mississippi, Ohio, and Lower Mississippi River basins. With 90% confidence, only a few watersheds can be reliably placed into the highest 150 category; however, many more watersheds can be removed from consideration as not belonging to the highest 150 category. Results from this ranking procedure provide robust information on watershed nutrient yields that can benefit management efforts to reduce nutrient loadings to downstream coastal waters, such as the Gulf of Mexico, or to local receiving streams and reservoirs.

  15. Culturing Selenastrum capricornutum (Chlorophyta) in a synthetic algal nutrient medium with defined mineral particulates

    USGS Publications Warehouse

    Kuwabara, J.S.; Davis, J.A.; Chang, Cecily C.Y.

    1985-01-01

    Algal nutrient studies in chemically-defined media typically employ a synthetic chelator to prevent iron hydroxide precipitation. Micronutrient-particulate interactions may, however, significantly affect chemical speciation and hence biovailability of these nutrients in natural waters. A technique is described by which Selenastrum capricornutum Printz (Chlorophyta) may be cultured in a medium where trace metal speciation (except iron) is controlled, not by organic chelation, but by sorption onto titanium dioxide. Application of this culturing protocol in conjunction with results from sorption studies of nutrient ions on mineral particles provides a means of studying biological impacts of sorptive processes in aquatic environments. ?? 1985 Dr W. Junk Publishers.

  16. Hydrologic processes and nutrient dynamics in a pristine mountain catchment

    USGS Publications Warehouse

    F. Richard Hauer,; Fagre, Daniel B.; Stanford, Jack A.

    2002-01-01

    Nutrient dynamics in watersheds have been used as an ecosystem-level indicator of overall ecosystem function or response to disturbance (e.g. Borman.N et al. 1974, WEBSTER et al. 1992). The examination of nutrients has been evaluated to determine responses to logging practices or other changes in watershed land use. Nutrient dynamics have been related to changing physical and biological characteristics (Mulholl AND 1992, CHESTNUT & McDowell 2000). Herein, the concentrations and dynamics of nitrogen, phosphorus and particulate organic carbon were examined in a large pristine watershed because they are affected by changes in discharge directly from the catchment and after passage through a large oligotrophic lake. 

  17. Aeolian nutrient fluxes following wildfire in sagebrush steppe: Implications for soil carbon storage

    USGS Publications Warehouse

    Hasselquist, N.J.; Germino, M.J.; Sankey, J.B.; Ingram, L.J.; Glenn, N.F.

    2011-01-01

    Pulses of aeolian transport following fire can profoundly affect the biogeochemical cycling of nutrients in semi-arid and arid ecosystems. Our objective was to determine horizontal nutrient fluxes occurring in the saltation zone during an episodic pulse of aeolian transport that occurred following a wildfire in a semi-arid sagebrush steppe ecosystem in southern Idaho, USA. We also examined how temporal trends in nutrient fluxes were affected by changes in particle sizes of eroded mass as well as nutrient concentrations associated with different particle size classes. In the burned area, total carbon (C) and nitrogen (N) fluxes were as high as 235 g C m????'1 d????'1 and 19 g N m????'1 d????'1 during the first few months following fire, whereas C and N fluxes were negligible in an adjacent unburned area throughout the study. Temporal variation in C and N fluxes following fire was largely attributable to the redistribution of saltation-sized particles. Total N and organic C concentrations in the soil surface were significantly lower in the burned relative to the unburned area one year after fire. Our results show how an episodic pulse of aeolian transport following fire can affect the spatial distribution of soil C and N, which, in turn, can have important implications for soil C storage. These findings demonstrate how an ecological disturbance can exacerbate a geomorphic process and highlight the need for further research to better understand the role aeolian transport plays in the biogeochemical cycling of C and N in recently burned landscapes. ?? Author(s) 2011. CC Attribution 3.0 License.

  18. Aeolian nutrient fluxes following wildfire in sagebrush steppe: Implications for soil carbon storage

    USGS Publications Warehouse

    Hasselquist, N.J.; Germino, M.J.; Sankey, J.B.; Ingram, L.J.; Glenn, N.F.

    2011-01-01

    Pulses of aeolian transport following fire can profoundly affect the biogeochemical cycling of nutrients in semi-arid and arid ecosystems. Our objective was to determine horizontal nutrient fluxes during an episodic pulse of aeolian transport that occurred following a wildfire in a semi-arid sagebrush steppe ecosystem in southern Idaho, USA. We also examined how temporal trends in nutrient fluxes were affected by changes in particle sizes of eroded mass as well as nutrient concentrations associated with different particle size classes. In the burned area, total carbon (C) and nitrogen (N) fluxes were as high as 235 g C m????'1 d????'1 and 19 g N m????'1 d????'1 during the first few months following fire, whereas C and N fluxes were negligible in an adjacent unburned area throughout the study. Temporal variation in C and N fluxes following fire was largely attributable to the redistribution of saltation-sized particles. Total N and organic C concentrations in the soil surface were significantly lower in the burned relative to the unburned area one year after fire. Our results show how an episodic pulse of aeolian transport following fire can affect the spatial distribution of soil C and N, which, in turn, can have important implications for soil C storage. These findings demonstrate how an ecological disturbance can exacerbate a geomorphic process and highlight the need for further research to better understand the role aeolian transport plays in the biogeochemical cycling of C and N in recently burned landscapes. ?? 2011 Author(s).

  19. Evolving nutritional strategies in the presence of competition: a geometric agent-based model.

    PubMed

    Senior, Alistair M; Charleston, Michael A; Lihoreau, Mathieu; Buhl, Jerome; Raubenheimer, David; Simpson, Stephen J

    2015-03-01

    Access to nutrients is a key factor governing development, reproduction and ultimately fitness. Within social groups, contest-competition can fundamentally affect nutrient access, potentially leading to reproductive asymmetry among individuals. Previously, agent-based models have been combined with the Geometric Framework of nutrition to provide insight into how nutrition and social interactions affect one another. Here, we expand this modelling approach by incorporating evolutionary algorithms to explore how contest-competition over nutrient acquisition might affect the evolution of animal nutritional strategies. Specifically, we model tolerance of nutrient excesses and deficits when ingesting nutritionally imbalanced foods, which we term 'nutritional latitude'; a higher degree of nutritional latitude constitutes a higher tolerance of nutritional excess and deficit. Our results indicate that a transition between two alternative strategies occurs at moderate to high levels of competition. When competition is low, individuals display a low level of nutritional latitude and regularly switch foods in search of an optimum. When food is scarce and contest-competition is intense, high nutritional latitude appears optimal, and individuals continue to consume an imbalanced food for longer periods before attempting to switch to an alternative. However, the relative balance of nutrients within available foods also strongly influences at what levels of competition, if any, transitions between these two strategies occur. Our models imply that competition combined with reproductive skew in social groups can play a role in the evolution of diet breadth. We discuss how the integration of agent-based, nutritional and evolutionary modelling may be applied in future studies to further understand the evolution of nutritional strategies across social and ecological contexts.

  20. Transformations of Heavy Metals and Plant Nutrients in Dredged Sediments as Affected by Oxidation Reduction Potential and pH. Volume 1. Literature Review

    DTIC Science & Technology

    1977-05-01

    895-896 (1974). 191. Fagerstrom, T., and Jernelov, A. "Formation of Methyl Mercury from Pure Mercuric Sulphide in Aerobic Organic Sediment." Water...was available. The toxic and nutrient elements included are lead, cadmium, mercury , arsenic, selenium, copper, zinc, manganese, iron, nitrogen...on the exchange of these materials between sediment and water. The toxic and nutrient elements included are lead, cadmium, mercury , ar- senic

  1. Impacts of urban wastewater discharge on seagrass meadows ( Zostera noltii)

    NASA Astrophysics Data System (ADS)

    Cabaço, Susana; Machás, Raquel; Vieira, Vasco; Santos, Rui

    2008-06-01

    The abiotic disturbance of urban wastewater discharge and its effects in the population structure, plant morphology, leaf nutrient content, epiphyte load and macroalgae abundance of Zostera noltii meadows were investigated in Ria Formosa coastal lagoon, southern Portugal using both univariate and multivariate analysis. Four sites were assessed, on a seasonal basis, along a gradient from a major Waste Water Treatment Works (WWTW) discharge to a main navigation channel. The wastewater discharge caused an evident environmental disturbance through the nutrient enrichment of the water and sediment, particularly of ammonium. Zostera noltii of the sites closest to the nutrient source showed higher leaf N content, clearly reflecting the nitrogen load. The anthropogenic nutrient enrichment resulted in higher biomass, and higher leaf and internode length, except for the meadow closest to the wastewater discharge (270 m). The high ammonium concentration (158-663 μM) in the water at this site resulted in the decrease of biomass, and both the leaf and internode length, suggesting a toxic effect on Z. noltii. The higher abundance of macroalgae and epiphytes found in the meadow closest to the nutrient source may also affect the species negatively. Shoot density was higher at the nutrient-undisturbed site. Two of the three abiotic processes revealed by Principal Component Analysis were clearly related to the WWTW discharge, a contrast between water column salinity and nutrient concentration and a sediment contrast between both porewater nutrients and temperature and redox potential. A multiple regression analysis showed that these abiotic processes had a significant effect on the biomass-density dynamics of meadows and on the overall size of Z. noltii plants, respectively. Results show that the wastewater discharge is an important source of environmental disturbance and nutrients availability in Ria Formosa lagoon affecting the population structure, morphology and N content of Z. noltii. This impact is spatially restricted to areas up to 600 m distant from the WWTW discharge, probably due to the high water renewal of the lagoon.

  2. Engineering crop nutrient efficiency for sustainable agriculture.

    PubMed

    Chen, Liyu; Liao, Hong

    2017-10-01

    Increasing crop yields can provide food, animal feed, bioenergy feedstocks and biomaterials to meet increasing global demand; however, the methods used to increase yield can negatively affect sustainability. For example, application of excess fertilizer can generate and maintain high yields but also increases input costs and contributes to environmental damage through eutrophication, soil acidification and air pollution. Improving crop nutrient efficiency can improve agricultural sustainability by increasing yield while decreasing input costs and harmful environmental effects. Here, we review the mechanisms of nutrient efficiency (primarily for nitrogen, phosphorus, potassium and iron) and breeding strategies for improving this trait, along with the role of regulation of gene expression in enhancing crop nutrient efficiency to increase yields. We focus on the importance of root system architecture to improve nutrient acquisition efficiency, as well as the contributions of mineral translocation, remobilization and metabolic efficiency to nutrient utilization efficiency. © 2017 Institute of Botany, Chinese Academy of Sciences.

  3. Macro-grazer herbivory regulates seagrass response to pulse and press nutrient loading.

    PubMed

    Ravaglioli, Chiara; Capocchi, Antonella; Fontanini, Debora; Mori, Giovanna; Nuccio, Caterina; Bulleri, Fabio

    2018-05-01

    Coastal ecosystems are exposed to multiple stressors. Predicting their outcomes is complicated by variations in their temporal regimes. Here, by means of a 16-month experiment, we investigated tolerance and resistance traits of Posidonia oceanica to herbivore damage under different regimes of nutrient loading. Chronic and pulse nutrient supply were combined with simulated fish herbivory, treated as a pulse stressor. At ambient nutrient levels, P. oceanica could cope with severe herbivory, likely through an increase in photosynthetic activity. Elevated nutrient levels, regardless of the temporal regime, negatively affected plant growth and increased leaf nutritional quality. This ultimately resulted in a reduction of plant biomass that was particularly severe under chronic fertilization. Our results suggest that both chronic and pulse nutrient loadings increase plant palatability to macro-grazers. Strategies for seagrass management should not be exclusively applied in areas exposed to chronic fertilization since even short-term nutrient pulses could alter seagrass meadows. Copyright © 2018 Elsevier Ltd. All rights reserved.

  4. Effect of vitamin supplementation on breast milk concentrations of retinol, carotenoids, and tocopherols in HIV-infected Tanzanian women

    PubMed Central

    Webb, Aimee L.; Aboud, Said; Furtado, Jeremy; Murrin, Clare; Campos, Hannia; Fawzi, Wafaie W.; Villamor, Eduardo

    2011-01-01

    Background The effect of daily prenatal and postnatal vitamin supplementation on concentrations of breast milk nutrients is not well characterized in HIV-infected women. Objective We examined the impact of vitamin supplementation during pregnancy and lactation on breast milk concentrations of retinol, carotenoids, and tocopherols during the first year post-partum among 626 HIV-infected Tanzanian women. Design We conducted a randomized, double-blind, placebo controlled trial. Women were assigned to one of four daily oral supplements: vitamin A + β-carotene (VA+BC); multivitamins (B, C, E (MV)); MV+VA+BC; or placebo. Concentrations of breast milk nutrients were determined by HPLC at birth and every 3 mo thereafter. Results Supplementation with VA+BC increased concentrations of retinol, β-carotene, and α-carotene at delivery by 4799, 1791, and 84 nmol/L, respectively, compared to no VA+BC (all p<0.0001). MV supplementation did not increase concentrations of α-tocopherol or δ-tocopherol at delivery but significantly decreased concentrations of breast milk γ-tocopherol and retinol. Although concentrations of all nutrients decreased significantly by 3 months postpartum, retinol, α-carotene, and β-carotene concentrations were significantly higher among those receiving VA+BC at 3, 6, and 12 mo compared to no VA+BC. Alpha tocopherol was significantly higher, while γ-tocopherol concentrations were significantly lower, among women receiving MV compared to no MV at 3, 6, and 12 mo post-partum. Conclusions Sustained supplementation of HIV-infected breastfeeding mothers with MV could be a safe and effective intervention to improve vitamin E concentrations in breast milk. VA+BC supplementation increases concentrations of breast milk retinol but it is not recommended in HIV-infected mothers due to the elevated risk of vertical transmission. PMID:17940544

  5. Effect of vitamin supplementation on breast milk concentrations of retinol, carotenoids and tocopherols in HIV-infected Tanzanian women.

    PubMed

    Webb, A L; Aboud, S; Furtado, J; Murrin, C; Campos, H; Fawzi, W W; Villamor, E

    2009-03-01

    The effect of daily prenatal and postnatal vitamin supplementation on concentrations of breast milk nutrients is not well characterized in HIV-infected women. We examined the impact of vitamin supplementation during pregnancy and lactation on breast milk concentrations of retinol, carotenoids and tocopherols during the first year postpartum among 626 HIV-infected Tanzanian women. We conducted a randomized, double-blind, placebo-controlled trial. Women were assigned to one of four daily oral supplements: vitamin A+beta-carotene (VA+BC); multivitamins (MV; B, C and E); MV+VA+BC or placebo. Concentrations of breast milk nutrients were determined by high-performance liquid chromatography at birth and every 3 months thereafter. Supplementation with VA+BC increased concentrations of retinol, beta-carotene and alpha-carotene at delivery by 4799, 1791 and 84 nmol l(-1), respectively, compared to no VA+BC (all P<0.0001). MV supplementation did not increase concentrations of alpha-tocopherol or delta-tocopherol at delivery but significantly decreased concentrations of breast milk gamma-tocopherol and retinol. Although concentrations of all nutrients decreased significantly by 3 months postpartum, retinol, alpha-carotene and beta-carotene concentrations were significantly higher among those receiving VA+BC at 3, 6 and 12 months compared to no VA+BC. alpha-Tocopherol was significantly higher, while gamma-tocopherol concentrations were significantly lower, among women receiving MV compared to no MV at 3, 6 and 12 months postpartum. Sustained supplementation of HIV-infected breastfeeding mothers with MV could be a safe and effective intervention to improve vitamin E concentrations in breast milk. VA+BC supplementation increases concentrations of breast milk retinol but it is not recommended in HIV-infected mothers due to the elevated risk of vertical transmission.

  6. The Influence of Shredder Feeding on Fungal Activity in a Nutrient-Enriched Stream and an Unaltered Stream

    NASA Astrophysics Data System (ADS)

    Chung, N.; Suberkopp, K.

    2005-05-01

    The effect of shredder feeding on aquatic hyphomycete communities associated with submerged leaves was studied in two southern Appalachian headwater streams in North Carolina. Coarse and fine mesh litter bags containing red maple (Acer rubrum) leaves were placed in the nutrient-enriched stream and in the reference stream and were retrieved monthly. Both shredder feeding and nutrient enrichment enhanced breakdown rates. The breakdown rates of leaves in coarse mesh bags in the reference stream (k = 0.0275) and fine mesh bags in the nutrient enriched stream (k = 0.0272) were not significantly different, suggesting that the shredding effect on litter breakdown was offset by higher fungal activity as a result of nutrient enrichment. Fungal sporulation rates and biomass (based on ergosterol concentrations) were higher in the nutrient enriched than in the reference stream, but neither fungal biomass nor sporulation rate was affected by shredder feeding. Species richness was higher in the nutrient-enriched than in the reference stream. The enrichment with nutrients altered fungal community composition more than shredder feeding.

  7. Evidence of chronic anthropogenic nutrient within coastal lagoon reefs adjacent to urban and tourism centers, Kenya: A stable isotope approach.

    PubMed

    Mwaura, Jelvas; Umezawa, Yu; Nakamura, Takashi; Kamau, Joseph

    2017-06-30

    The source of anthropogenic nutrient and its spatial extent in three fringing reefs with differing human population gradients in Kenya were investigated using stable isotope approaches. Nutrient concentrations and nitrate-δ 15 N in seepage water indicated that population density and tourism contributed greatly to the extent of nutrient loading to adjacent reefs. Although water-column nutrient analyses did not show any significant difference among the reefs, higher δ 15 N and N contents in macrophytes showed terrestrial nutrients affected primary producers in onshore areas in Nyali and Bamburi reefs, but were mitigated by offshore water intrusion especially at Nyali. On the offshore reef flat, where the same species of macroalgae were not available, complementary use of δ 15 N in sedimentary organic matter suggested inputs of nutrients originated from the urban city of Mombasa. If population increases in the future, nutrient conditions in the shallower reef, Vipingo, may be dramatically degraded due to lower water exchange ratio. Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. Local geology determines responses of stream producers and fungal decomposers to nutrient enrichment: A field experiment.

    PubMed

    Mykrä, Heikki; Sarremejane, Romain; Laamanen, Tiina; Karjalainen, Satu Maaria; Markkola, Annamari; Lehtinen, Sirkku; Lehosmaa, Kaisa; Muotka, Timo

    2018-04-16

    We examined how short-term (19 days) nutrient enrichment influences stream fungal and diatom communities, and rates of leaf decomposition and algal biomass accrual. We conducted a field experiment using slow-releasing nutrient pellets to increase nitrate (NO 3 -N) and phosphate (PO 4 -P) concentrations in a riffle section of six naturally acidic (naturally low pH due to catchment geology) and six circumneutral streams. Nutrient enrichment increased microbial decomposition rate on average by 14%, but the effect was significant only in naturally acidic streams. Nutrient enrichment also decreased richness and increased compositional variability of fungal communities in naturally acidic streams. Algal biomass increased in both stream types, but algal growth was overall very low. Diatom richness increased in response to nutrient addition by, but only in circumneutral streams. Our results suggest that primary producers and decomposers are differentially affected by nutrient enrichment and that their responses to excess nutrients are context dependent, with a potentially stronger response of detrital processes and fungal communities in naturally acidic streams than in less selective environments.

  9. Drainage and fertilization effects on nutrient availability in an ombrotrophic peatland.

    PubMed

    Wang, Meng; Talbot, Julie; Moore, Tim R

    2018-04-15

    Nutrient availability is an important control on the vegetation distribution, productivity and functioning of peatland ecosystems and we examined spatial and temporal patterns of nutrient availability through ion exchange at Mer Bleue bog, southeast Ontario, Canada. We installed ion exchange probes at 5-15cm for 4weeks and determined nutrient sorption at undisturbed sites as well as those affected by nitrogen (N), phosphorus (P), potassium (K) fertilization and drainage. Under undisturbed conditions, the bog had very small amount of available nutrients, especially N (ammonium>nitrate) and P, and exhibited small variations in nutrient availability during the growing season (May to October). The increase in NPK availability upon fertilization was short-lived over the season and the stoichiometry of available NPK captured by the probes was mismatched with the vegetation. The increase in nutrient availability with drainage was confounded by substantial changes in vegetation. We compare these results with data from other Canadian bogs and fens to provide baseline data on nutrient availability in peatlands. Copyright © 2017 Elsevier B.V. All rights reserved.

  10. Nutrient Presses and Pulses Differentially Impact Plants, Herbivores, Detritivores and Their Natural Enemies

    PubMed Central

    Murphy, Shannon M.; Wimp, Gina M.; Lewis, Danny

    2012-01-01

    Anthropogenic nutrient inputs into native ecosystems cause fluctuations in resources that normally limit plant growth, which has important consequences for associated food webs. Such inputs from agricultural and urban habitats into nearby natural systems are increasing globally and can be highly variable, spanning the range from sporadic to continuous. Despite the global increase in anthropogenically-derived nutrient inputs into native ecosystems, the consequences of variation in subsidy duration on native plants and their associated food webs are poorly known. Specifically, while some studies have examined the effects of nutrient subsidies on native ecosystems for a single year (a nutrient pulse), repeated introductions of nutrients across multiple years (a nutrient press) better reflect the persistent nature of anthropogenic nutrient enrichment. We therefore contrasted the effects of a one-year nutrient pulse with a four-year nutrient press on arthropod consumers in two salt marshes. Salt marshes represent an ideal system to address the differential impacts of nutrient pulses and presses on ecosystem and community dynamics because human development and other anthropogenic activities lead to recurrent introductions of nutrients into these natural systems. We found that plant biomass and %N as well as arthropod density fell after the nutrient pulse ended but remained elevated throughout the nutrient press. Notably, higher trophic levels responded more strongly than lower trophic levels to fertilization, and the predator/prey ratio increased each year of the nutrient press, demonstrating that food web responses to anthropogenic nutrient enrichment can take years to fully manifest themselves. Vegetation at the two marshes also exhibited an apparent tradeoff between increasing %N and biomass in response to fertilization. Our research emphasizes the need for long-term, spatially diverse studies of nutrient enrichment in order to understand how variation in the duration of anthropogenic nutrient subsidies affects native ecosystems. PMID:22952814

  11. Patient Involvement in Safe Delivery: A Qualitative Study.

    PubMed

    Olfati, Forozun; Asefzadeh, Saeid; Changizi, Nasrin; Keramat, Afsaneh; Yunesian, Masud

    2015-09-28

    Patient involvement in safe delivery planning is considered important yet not widely practiced. The present study aimed at identifythe factors that affect patient involvementin safe delivery, as recommended by parturient women. This study was part of a qualitative research conducted by content analysis method and purposive sampling in 2013.The data were collected through 63 semi-structured interviews in4 hospitalsand analyzed using thematic content analysis. The participants in this research were women before discharge and after delivery. Findings were analyzed using Colaizzi's method. Four categories of factors that could affect patient involvement in safe delivery emerged from our analysis: patient-related (true and false beliefs, literacy, privacy, respect for patient), illness-related (pain, type of delivery, patient safety incidents), health care professional-relatedand task-related factors (behavior, monitoring &training), health care setting-related (financial aspects, facilities). More research is needed to explore the factors affecting the participation of mothers. It is therefore, recommended to: 1) take notice of mother education, their husbands, midwives and specialists; 2) provide pregnant women with insurance coverage from the outset of pregnancy, especially during prenatal period; 3) form a labor pain committee consisting of midwives, obstetricians, and anesthesiologists in order to identify the preferred painless labor methods based on the existing facilities and conditions, 4) carry out research on observing patients' privacy and dignity; 5) pay more attention on the factors affecting cesarean.

  12. The effect of nutrient-based standards on competitive foods in 3 schools: potential savings in kilocalories and grams of fat.

    PubMed

    Snelling, Anastasia M; Yezek, Jennifer

    2012-02-01

    The study investigated how nutrient standards affected the number of kilocalories and grams of fat and saturated fat in competitive foods offered and sold in 3 high schools. The study is a quasi-experimental design with 3 schools serving as the units of assignment and analysis. The effect of the nutrient standards was measured by the change in kilocalories and grams of fat and saturated fat in offerings and purchases of competitive foods pre- and postimplementation of the standards. A paired sample t-test was used to compare kilocalories and grams of fat and saturated fat pre- and postimplementation of nutrition standards. After the implementation of the nutrition standards, students in 3 high schools purchased significantly smaller numbers of kilocalories and grams of fat and saturated fat, during the postpolicy school year of 2007-2008 than during the prepolicy school year of 2004-2005. Using nutrient standards to guide the selection of competitive foods offered in school cafeterias may positively affect intake of kilocalories, total grams of fat, and total saturated fat of those foods. The quantitative assessment is novel and demonstrates the reduction in kilocalories and fat in both the competitive food offerings and purchases as a result of nutrient standards. © 2012, American School Health Association.

  13. Variations in Amazon forest productivity correlated with foliar nutrients and modelled rates of photosynthetic carbon supply

    PubMed Central

    Mercado, Lina M.; Patiño, Sandra; Domingues, Tomas F.; Fyllas, Nikolaos M.; Weedon, Graham P.; Sitch, Stephen; Quesada, Carlos Alberto; Phillips, Oliver L.; Aragão, Luiz E. O. C.; Malhi, Yadvinder; Dolman, A. J.; Restrepo-Coupe, Natalia; Saleska, Scott R.; Baker, Timothy R.; Almeida, Samuel; Higuchi, Niro; Lloyd, Jon

    2011-01-01

    The rate of above-ground woody biomass production, WP, in some western Amazon forests exceeds those in the east by a factor of 2 or more. Underlying causes may include climate, soil nutrient limitations and species composition. In this modelling paper, we explore the implications of allowing key nutrients such as N and P to constrain the photosynthesis of Amazon forests, and also we examine the relationship between modelled rates of photosynthesis and the observed gradients in WP. We use a model with current understanding of the underpinning biochemical processes as affected by nutrient availability to assess: (i) the degree to which observed spatial variations in foliar [N] and [P] across Amazonia affect stand-level photosynthesis; and (ii) how these variations in forest photosynthetic carbon acquisition relate to the observed geographical patterns of stem growth across the Amazon Basin. We find nutrient availability to exert a strong effect on photosynthetic carbon gain across the Basin and to be a likely important contributor to the observed gradient in WP. Phosphorus emerges as more important than nitrogen in accounting for the observed variations in productivity. Implications of these findings are discussed in the context of future tropical forests under a changing climate. PMID:22006971

  14. On-the-Move Nutrient Delivery System Performance Characteristics

    DTIC Science & Technology

    2008-09-01

    types - ranging from simple sugar (monosaccharide fructose or disaccharide sucrose) to more complex sugars (short length maltodextrin (Grain...characteristics of the NOS Position Chest Chest Chest Pressure, Flow Rate, Glucose Conc in Sip-to-Sip Estimated CHO mm Hg Setting Time, s Volume, ml...on the drink produced (Table 2). When the top of the concentrate bag was level with the bite valve, the drink had an estimated carbohydrate

  15. Effects of Fe deficiency on the protein profiles and lignin composition of stem tissues of Medicago truncatula in absence and presence of calcium carbonate

    USDA-ARS?s Scientific Manuscript database

    Iron deficiency is a yield-limiting factor with major implications for crop production, especially in soils with high CaCO3. Because stems are essential for the delivery of nutrients to the shoots, the aim of this work was to study the effects of Fe deficiency on the stem proteome of Medicago trunca...

  16. Wildfire Effects on In-stream Nutrient Processing and Hydrologic Transport

    NASA Astrophysics Data System (ADS)

    Rhea, A.; Covino, T. P.; Rhoades, C.; Fegel, T.

    2017-12-01

    In many forests throughout the Western U.S., drought, climate change, and growing fuel loads are contributing to increased fire frequency and severity. Wildfires can influence watershed nutrient retention as they fundamentally alter the biological composition and physical structure in upland landscapes, riparian corridors, and stream channels. While numerous studies have documented substantial short-term increases in stream nutrient concentrations and export (particularly reactive nitrogen, N) following forest fires, the long-term implications for watershed nutrient cycling remain unclear. For example, recent work indicates that nitrate concentrations and export can remain elevated for a decade or more following wildfire, yet the controls on these processes are unknown. In this research, we use empirical observations from nutrient tracer injections, nutrient diffusing substrates, and continuous water quality monitoring to isolate biological and physical controls on nutrient export across a burn-severity gradient. Tracer results demonstrate substantial stream-groundwater exchange, but little biological nutrient uptake in burned streams. This in part explains patterns of elevated nutrient export. Paired nutrient diffusing substrate experiments allow us to further investigate shifts in N, phosphorus, and carbon limitation that may suppress post-fire stream nutrient uptake. By isolating the mechanisms that reduce the capacity of fire-affected streams to retain and transform nutrient inputs, we can better predict dynamics in post-fire water quality and help prioritize upland and riparian restoration.

  17. Differences in carotenoid accumulation among three feeder-cricket species: implications for carotenoid delivery to captive insectivores.

    PubMed

    Ogilvy, Victoria; Fidgett, Andrea L; Preziosi, Richard F

    2012-01-01

    There are a limited number of feeder-invertebrates available to feed captive insectivores, and many are deficient in certain nutrients. Gut-loading is used to increase the diversity of nutrients present in the captive insectivore diet; however, little is known about delivery of carotenoids via gut-loading. Carotenoids may influence health and reproduction due to their roles in immune and antioxidant systems. We assessed interspecific variation in carotenoid accumulation and retention in three feeder-cricket species (Gryllus bimaculatus, Gryllodes sigillatus and Acheta domesticus) fed one of three diets (wheat-bran, fish-food based formulated diet, and fresh fruit and vegetables). Out of the three species of feeder-cricket in the fish-food-based dietary treatment group, G. bimaculatus had the greatest total carotenoid concentration. All cricket species fed the wheat-bran diet had very low carotenoid concentrations. Species on the fish-food-based diet had intermediate carotenoid concentrations, and those on the fruit and vegetable diet had the highest concentrations. Carotenoid retention was poor across all species. Overall, this study shows that, by providing captive insectivores with G. bimaculatus crickets recently fed a carotenoid-rich diet, the quantity of carotenoids in the diet can be increased. © 2011 Wiley Periodicals, Inc.

  18. Closed Ecological Life Support Systems (CELSS) Test Facility

    NASA Technical Reports Server (NTRS)

    Macelroy, Robert D.

    1992-01-01

    The CELSS Test Facility (CTF) is being developed for installation on Space Station Freedom (SSF) in August 1999. It is designed to conduct experiments that will determine the effects of microgravity on the productivity of higher (crop) plants. The CTF will occupy two standard SSF racks and will accommodate approximately one square meter of growing area and a canopy height of 80 cm. The growth volume will be isolated from the external environment, allowing stringent control of environmental conditions. Temperature, humidity, oxygen, carbon dioxide, and light levels will all be closely controlled to prescribed set points and monitored. This level of environmental control is needed to prevent stress and allow accurate assessment of microgravity effect (10-3 to 10-6 x g). Photosynthetic rates and respiration rates, calculated through continuous recording of gas concentrations, transpiration, and total and edible biomass produced will be measured. Toxic byproducts will be monitored and scrubbed. Transpiration water will be collected within the chamber and recycled into the nutrient solution. A wide variety of crop plants, e.g., wheat, soy beans, lettuce, potatoes, can be accommodated and various nutrient delivery systems and light delivery systems will be available. In the course of its development, the CTF will exploit fully, and contribute importantly, to the state-of-art in closed system technology and plant physiology.

  19. Flexible Microsensor Array for the Root Zone Monitoring of Porous Tube Plant Growth System

    NASA Technical Reports Server (NTRS)

    Sathyan, Sandeep; Kim, Chang-Soo; Porterfield, D. Marshall; Nagle, H. Troy; Brown, Christopher S.

    2004-01-01

    Control of oxygen and water in the root zone is vital to support plant growth in the microgravity environment. The ability to control these sometimes opposing parameters in the root zone is dependent upon the availability of sensors to detect these elements and provide feedback for control systems. In the present study we demonstrate the feasibility of using microsensor arrays on a flexible substrate for dissolved oxygen detection, and a 4-point impedance microprobe for surface wetness detection on the surface of a porous tube (PT) nutrient delivery system. The oxygen microsensor reported surface oxygen concentrations that correlated with the oxygen concentrations of the solution inside the PT when operated at positive pressures. At negative pressures the microsensor shows convergence to zero saturation (2.2 micro mol/L) values due to inadequate water film formation on porous tube surface. The 4-point microprobe is useful as a wetness detector as it provides a clear differentiation between dry and wet surfaces. The unique features of the dissolved oxygen microsensor array and 4-point microprobe include small and simple design, flexibility and multipoint sensing. The demonstrated technology is anticipated to provide low cost, and highly reliable sensor feedback monitoring plant growth nutrient delivery system in both terrestrial and microgravity environments.

  20. Engineering strategies for the design of plant nutrient delivery systems for use in space: approaches to countering microbiological contamination

    NASA Technical Reports Server (NTRS)

    Gonzales, A. A.; Schuerger, A. C.; Barford, C.; Mitchell, R.

    1996-01-01

    Microbiological contamination of crops within space-based plant growth research chambers has been postulated as a potentially significant problem. Microbial infestations; fouling of Nutrient Delivery System (NDS) fluid loops; and the formation of biofilms have been suggested as the most obvious and important manifestations of the problem. Strict sanitation and quarantine procedures will reduce, but not eliminate, microbial species introduced into plant growth systems in space habitats. Microorganisms transported into space most likely will occur as surface contaminants on spacecraft components, equipment, the crew, and plant-propagative materials. Illustrations of the potential magnitude of the microbiological contamination issue will be drawn from the literature and from documentation of laboratory and commercial field experience. Engineering strategies for limiting contamination and for the development of countermeasures will be described. Microbiological control technologies and NDS hardware will be discussed. Configurations appropriate for microgravity research facilities, as well as anticipated bio-regenerative life support system implementations, will be explored. An efficiently designed NDS, capable of adequately meeting the environmental needs of crop plants in space, is considered to be critical in both the research and operational domains. Recommended experiments, tests, and technology developments, structured to allow the development of prudent engineering solutions also will be presented.

  1. Engineering Strategies for the Design of Plant Nutrient Delivery Systems for Use in Space: Approaches to Countering Microbiological Contamination

    NASA Technical Reports Server (NTRS)

    Gonzales, A. A.; Schuerger, A. C.; Mitchell, R.; Harper, Lynn D. (Technical Monitor)

    1994-01-01

    Microbiological contamination of crops within space-based crop growth research chambers has been postulated as a potentially significant problem. Microbial infestations; fouling of Nutrient Delivery System (NDS) fluid loops; and the formation of biofilms, have been suggested as the most obvious and important manifestations of the problem. Strict sanitation and quarantine procedures will reduce, but not eliminate, microbial species introduced into plant growing systems in space habitats. Microorganisms transported into space will most likely occur as contaminants on spacecraft components, equipment, the crew, and plant-propagative materials. Illustrations of the potential magnitude of the microbiological contamination issue will be drawn from the literature and from documentation of laboratory and commercial field experience. Engineering strategies for limiting contamination and for the development of countermeasures will be described. Microbiological control technologies and NDS hardware will be discussed. Configurations appropriate for microgravity research facilities, as well as anticipated bio-regenerative life support system implementations, will be explored. An efficiently designed NDS, capable of adequately meeting the environmental needs of crop plants in space, is considered to be critical in both the research and operational domains. Recommended experiments, tests and technology developments, structured to allow the development of prudent engineering solutions, will also be presented.

  2. Soil properties, soil functions and soil security

    NASA Astrophysics Data System (ADS)

    Poggio, Laura; Gimona, Alessandro

    2017-04-01

    Soil plays a crucial role in the ecosystem functioning such as food production, capture and storage of water, carbon and nutrients and in the realisation of a number of UN Sustainable Developments Goals. In this work we present an approach to spatially and jointly assess the multiple contributions of soil to the delivery of ecosystem services within multiple land-use system. We focussed on the modelling of the impact of soil on sediment retention, carbon storage, storing and filtering of nutrients, habitat for soil organisms and water regulation, taking into account examples of land use and climate scenarios. Simplified models were used for the single components. Spatialised Bayesian Belief networks were used for the jointly assessment and mapping of soil contribution to multiple land use and ecosystem services. We integrated continuous 3D soil information derived from digital soil mapping approaches covering the whole of mainland Scotland, excluding the Northern Islands. Uncertainty was accounted for and propagated across the whole process. The Scottish test case highlights the differences in roles between mineral and organic soils and provides an example of integrated study assessing the contributions of soil. The results show the importance of the multi-functional analysis of the contribution of soils to the ecosystem service delivery and UN SDGs.

  3. Drosophila as a model for epithelial tube formation.

    PubMed

    Maruyama, Rika; Andrew, Deborah J

    2012-01-01

    Epithelial tubular organs are essential for life in higher organisms and include the pancreas and other secretory organs that function as biological factories for the synthesis and delivery of secreted enzymes, hormones, and nutrients essential for tissue homeostasis and viability. The lungs, which are necessary for gas exchange, vocalization, and maintaining blood pH, are organized as highly branched tubular epithelia. Tubular organs include arteries, veins, and lymphatics, high-speed passageways for delivery and uptake of nutrients, liquids, gases, and immune cells. The kidneys and components of the reproductive system are also epithelial tubes. Both the heart and central nervous system of many vertebrates begin as epithelial tubes. Thus, it is not surprising that defects in tube formation and maintenance underlie many human diseases. Accordingly, a thorough understanding how tubes form and are maintained is essential to developing better diagnostics and therapeutics. Among the best-characterized tubular organs are the Drosophila salivary gland and trachea, organs whose relative simplicity have allowed for in depth analysis of gene function, yielding key mechanistic insight into tube initiation, remodeling and maintenance. Here, we review our current understanding of salivary gland and trachea formation - highlighting recent discoveries into how these organs attain their final form and function. Copyright © 2011 Wiley Periodicals, Inc.

  4. ECOLOGICAL AND WATER QUALITY CONSEQUENCES OF NUTRIENT ADDITION FOR SALMON RESTORATION IN THE PACIFIC NORTHWEST OF NORTH AMERICA

    EPA Science Inventory

    Salmon runs have declined over the past two centuries in the Pacific Northwest region of North America. Reduced inputs of salmon-derived organic matter and nutrients (SDN) may limit freshwater production and thus establish a negative feedback loop affecting future generations of...

  5. Weed management, training, and irrigation practices for organic production of trailing blackberry: II. Soil and plant nutrient concentrations

    USDA-ARS?s Scientific Manuscript database

    Organic production of blackberries is increasing, but there is relatively little known about how production practices affect plant and soil nutrient status. The impact of cultivar (‘Black Diamond’ and ‘Marion’), weed management (weed mat, hand weeding, and no weeding), primocane training time (Augus...

  6. A mathematical model of the effect of a predator on species diversity

    NASA Technical Reports Server (NTRS)

    Weston, C. R.; Yang, J. N.

    1970-01-01

    Mathematical model determines reaction between new predator and microbe competitor when the competitor is the predator's sole nutrient resource. The model utilizes differential equations to describe the interactions with the specific growth rates, and analyzes these growth rates as they are affected by population density and nutrient concentration.

  7. Runoff nutrient transport as affected by land application method, swine growth stage, and runoff rate

    USDA-ARS?s Scientific Manuscript database

    This study was conducted to measure the effects of slurry application method, swine growth stage, and flow rate on runoff nutrient transport. Swine slurry was obtained from production units containing grower pigs, finisher pigs, or sows and gilts. The swine slurry was applied using broadcast, disk, ...

  8. The Use of Nutritive Composition Data at the Point of Purchase.

    ERIC Educational Resources Information Center

    Muller, Thomas E.

    1984-01-01

    Determined whether shoppers would use nutrition information when making in-store choices among brands of five packaged products. Also determined whether information used would be affected by the number of nutrients appearing on signs displayed in the store, and/or by the perceived importance of the set of nutrients presented. (JN)

  9. Composting of biochars improves their sorption properties, retains nutrients during composting and affects greenhouse gas emissions after soil application

    USDA-ARS?s Scientific Manuscript database

    Biochar application to soils has been suggested to elevate nutrient sorption, improve soil fertility and reduce net greenhouse gas (GHG) emissions. We examined the impact of composting biochar together with a biologically active substrate (i.e., livestock manure-straw mixture). We hypothesized that ...

  10. Effect of multiple stressors on eelgrass Zostera marina L. from the Pacific Northwest, USA: Manipulation of temperature and nutrients

    EPA Science Inventory

    Estuarine eelgrass beds in the Pacific Northwest (PNW) are being exposed to a range of natural and anthropogenic stressors and climate change. These stresses include increased temperatures, sea level rise, and high nutrient inputs, all of which may directly affect the productivi...

  11. Nutrient enrichment reduces constraints on material flows in a detritus-based food web

    Treesearch

    Wyatt F. Cross; Bruce Wallace; Amy D. Rosemond

    2007-01-01

    Most aquatic and terrestrial ecosystems are experiencing increased nutrient availability, which is affecting their structure and function. By altering community composition and productivity of consumers, enrichment can indirectly cause changes in the pathways and magnitude of material flows in food webs. These changes, in turn, have major consequences for material...

  12. Does cattle grazing of dual-purpose wheat accelerate the rate of stubble decomposition and nutrients released

    USDA-ARS?s Scientific Manuscript database

    Decomposition and nutrient release of winter annual forages in integrated crop-livestock systems could be affected by the resultant alterations in structure and quality of residues caused by grazing, but little information is available to test this hypothesis. Information on residue dynamics is need...

  13. Practical and clinical nutritional concerns during spaceflight.

    PubMed

    Seddon, M R; Fettman, M J; Phillips, R W

    1994-11-01

    Experience with space exploration to date has raised more questions regarding nutritional requirements for astronauts than it has answered. As mission lengths continue to increase, nutrient imbalances due to alterations in intake, dietary requirements, bioavailability, or excretion, may become more important. Factors adversely affecting intake include those as straightforward as stress and as complex as space-adaptation syndrome. Metabolic alterations induced by shifts in fluid and electrolyte balance, neuroendocrine function, and changes in hepatic protein synthesis and skeletal muscle type that result in nutrient partitioning to different biochemical pathways may also affect dietary requirements. Food processing effects on nutrient stability and digestibility, which apply to limited quantities of our usual diet on Earth, may become more important for diets that contain little fresh food during extended-length missions. Whereas nutrient and water recycling through ecosystems is taken for granted on Earth, specific effects of trace contaminant accumulation will require greater attention for prolonged space flights. Human factors, esthetics, and user-friendly operations will be necessary to facilitate the psychological as well as physiological health of the astronauts.

  14. Practical and clinical nutritional concerns during spaceflight

    NASA Technical Reports Server (NTRS)

    Seddon, M. R.; Fettman, M. J.; Phillips, R. W.

    1994-01-01

    Experience with space exploration to date has raised more questions regarding nutritional requirements for astronauts than it has answered. As mission lengths continue to increase, nutrient imbalances due to alterations in intake, dietary requirements, bioavailability, or excretion, may become more important. Factors adversely affecting intake include those as straightforward as stress and as complex as space-adaptation syndrome. Metabolic alterations induced by shifts in fluid and electrolyte balance, neuroendocrine function, and changes in hepatic protein synthesis and skeletal muscle type that result in nutrient partitioning to different biochemical pathways may also affect dietary requirements. Food processing effects on nutrient stability and digestibility, which apply to limited quantities of our usual diet on Earth, may become more important for diets that contain little fresh food during extended-length missions. Whereas nutrient and water recycling through ecosystems is taken for granted on Earth, specific effects of trace contaminant accumulation will require greater attention for prolonged space flights. Human factors, esthetics, and user-friendly operations will be necessary to facilitate the psychological as well as physiological health of the astronauts.

  15. Chronic human disturbance affects plant trait distribution in a seasonally dry tropical forest

    NASA Astrophysics Data System (ADS)

    Sfair, Julia C.; de Bello, Francesco; de França, Thaysa Q.; Baldauf, Cristina; Tabarelli, Marcelo

    2018-02-01

    The effects of human disturbance on biodiversity can be mediated by environmental conditions, such as water availability, climate and nutrients. In general, disturbed, dry or nutrient-depleted soils areas tend to have lower taxonomic diversity. However, little is known about how these environmental conditions affect functional composition and intraspecific variability in tropical dry forests. We studied a seasonally dry tropical forest (SDTF) under chronic anthropogenic disturbance (CAD) along rainfall and soil nutrient gradients to understand how these factors influence the taxonomic and functional composition. Specifically we evaluated two aspects of CAD, wood extraction and livestock pressure (goat and cattle grazing), along soil fertility and rainfall gradients on shrub and tree traits, considering species turnover and intraspecific variability. In addition, we also tested how the traits of eight populations of the most frequent species are affected by wood extraction, livestock pressure, rainfall and soil fertility. In general, although CAD and environmental gradients affected each trait of the most widespread species differently, the most abundant species also had a greater variation of traits. Considering species turnover, wood extraction is associated with species with a smaller leaf area and lower investment in leaf mass, probably due to the indirect effects of this disturbance type on the vegetation, i.e. the removal of branches and woody debris clears the vegetation, favouring species that minimize water loss. Livestock pressure, on the other hand, affected intraspecific variation: the herbivory caused by goats and cattle promoted individuals which invest more in wood density and leaf mass. In this case, the change of functional composition observed is a direct effect of the disturbance, such as the decrease of palatable plant abundance by goat and cattle herbivory. In synthesis, CAD, rainfall and soil fertility can affect trait distribution at community and species levels, which can have significant implications for the ecosystem functioning of SDTF under increasing levels of disturbance, climate change and soil nutrient depletion.

  16. Plant growth improvement mediated by nitrate capture in co-composted biochar

    PubMed Central

    Kammann, Claudia I.; Schmidt, Hans-Peter; Messerschmidt, Nicole; Linsel, Sebastian; Steffens, Diedrich; Müller, Christoph; Koyro, Hans-Werner; Conte, Pellegrino; Stephen, Joseph

    2015-01-01

    Soil amendment with pyrogenic carbon (biochar) is discussed as strategy to improve soil fertility to enable economic plus environmental benefits. In temperate soils, however, the use of pure biochar mostly has moderately-negative to -positive yield effects. Here we demonstrate that co-composting considerably promoted biochars’ positive effects, largely by nitrate (nutrient) capture and delivery. In a full-factorial growth study with Chenopodium quinoa, biomass yield increased up to 305% in a sandy-poor soil amended with 2% (w/w) co-composted biochar (BCcomp). Conversely, addition of 2% (w/w) untreated biochar (BCpure) decreased the biomass to 60% of the control. Growth-promoting (BCcomp) as well as growth-reducing (BCpure) effects were more pronounced at lower nutrient-supply levels. Electro-ultra filtration and sequential biochar-particle washing revealed that co-composted biochar was nutrient-enriched, particularly with the anions nitrate and phosphate. The captured nitrate in BCcomp was (1) only partly detectable with standard methods, (2) largely protected against leaching, (3) partly plant-available, and (4) did not stimulate N2O emissions. We hypothesize that surface ageing plus non-conventional ion-water bonding in micro- and nano-pores promoted nitrate capture in biochar particles. Amending (N-rich) bio-waste with biochar may enhance its agronomic value and reduce nutrient losses from bio-wastes and agricultural soils. PMID:26057083

  17. Plant growth improvement mediated by nitrate capture in co-composted biochar

    NASA Astrophysics Data System (ADS)

    Kammann, Claudia I.; Schmidt, Hans-Peter; Messerschmidt, Nicole; Linsel, Sebastian; Steffens, Diedrich; Müller, Christoph; Koyro, Hans-Werner; Conte, Pellegrino; Stephen, Joseph

    2015-06-01

    Soil amendment with pyrogenic carbon (biochar) is discussed as strategy to improve soil fertility to enable economic plus environmental benefits. In temperate soils, however, the use of pure biochar mostly has moderately-negative to -positive yield effects. Here we demonstrate that co-composting considerably promoted biochars’ positive effects, largely by nitrate (nutrient) capture and delivery. In a full-factorial growth study with Chenopodium quinoa, biomass yield increased up to 305% in a sandy-poor soil amended with 2% (w/w) co-composted biochar (BCcomp). Conversely, addition of 2% (w/w) untreated biochar (BCpure) decreased the biomass to 60% of the control. Growth-promoting (BCcomp) as well as growth-reducing (BCpure) effects were more pronounced at lower nutrient-supply levels. Electro-ultra filtration and sequential biochar-particle washing revealed that co-composted biochar was nutrient-enriched, particularly with the anions nitrate and phosphate. The captured nitrate in BCcomp was (1) only partly detectable with standard methods, (2) largely protected against leaching, (3) partly plant-available, and (4) did not stimulate N2O emissions. We hypothesize that surface ageing plus non-conventional ion-water bonding in micro- and nano-pores promoted nitrate capture in biochar particles. Amending (N-rich) bio-waste with biochar may enhance its agronomic value and reduce nutrient losses from bio-wastes and agricultural soils.

  18. Rapid alkalization in Lake Inawashiro, Fukushima, Japan: implications for future changes in the carbonate system of terrestrial waters

    NASA Astrophysics Data System (ADS)

    Manaka, T.; Ushie, H.; Araoka, D.; Inamura, A.; Suzuki, A.; Kawahata, H.

    2013-12-01

    The global carbon cycle, one of the important biogeochemical cycles controlling the surface environment of the Earth, has been greatly affected by human activity. Anthropogenic nutrient loading from urban sewage and agricultural runoff has caused eutrophication of aquatic systems. The impact of this eutrophication and consequent photosynthetic activity on CO2 exchange between freshwater systems and the atmosphere is unclear. In this study, we focused on how nutrient loading to lakes affects their carbonate system. Here, we report results of surveys of lakes in Japan at different stages of eutrophication. Alkalization due to photosynthetic activity and decreases in PCO2 had occurred in eutrophic lakes (e.g., Lake Kasumigaura), whereas in an acidotrophic lake (Lake Inawashiro) that was impacted by volcanic hot springs, nutrient loading was changing the pH and carbon cycling. When the influence of volcanic activity was stronger in the past in Lake Inawashiro, precipitation of volcanic-derived iron and aluminum had removed nutrients by co-precipitation. During the last three decades, volcanic activity has weakened and the lake water has become alkalized. We inferred that this rapid alkalization did not result just from the reduction in acid inputs but was also strongly affected by increased photosynthetic activity during this period. Human activities affect many lakes in the world. These lakes may play an important part in the global carbon cycle through their influence on CO2 exchange between freshwater and the atmosphere. Biogeochemical changes and processes in these systems have important implications for future changes in aquatic carbonate systems on land.

  19. Combined Effects of Dissolved Nutrients and Oxygen on Plant Litter Decomposition and Associated Fungal Communities.

    PubMed

    Gomes, Patrícia Pereira; Ferreira, Verónica; Tonin, Alan M; Medeiros, Adriana Oliveira; Júnior, José Francisco Gonçalves

    2018-05-01

    Aquatic ecosystems worldwide have been substantially altered by human activities, which often induce changes in multiple factors that can interact to produce complex effects. Here, we evaluated the combined effects of dissolved nutrients (nitrogen [N] and phosphorus [P]; three levels: concentration found in oligotrophic streams in the Cerrado biome, 10× and 100× enriched) and oxygen (O 2 ; three levels: hypoxic [4% O 2 ], depleted [55% O 2 ], and saturated [96% O 2 ]) on plant litter decomposition and associated fungal decomposers in laboratory microcosms simulating stream conditions under distinct scenarios of water quality deterioration. Senescent leaves of Maprounea guianensis were incubated for 10 days in an oligotrophic Cerrado stream to allow microbial colonization and subsequently incubated in microcosms for 21 days. Leaves lost 1.1-3.0% of their initial mass after 21 days, and this was not affected either by nutrients or oxygen levels. When considering simultaneous changes in nutrients and oxygen concentrations, simulating increased human pressure, fungal biomass accumulation, and sporulation rates were generally inhibited. Aquatic hyphomycete community structure was also affected by changes in nutrients and oxygen availability, with stronger effects found in hypoxic treatments than in depleted or saturated oxygen treatments. This study showed that the effects of simultaneous changes in the availability of dissolved nutrients and oxygen in aquatic environments can influence the activity and composition of fungal communities, although these effects were not translated into changes in litter decomposition rates.

  20. Nutrient concentrations within and below root zones from applied chicken manure in selected Hawaiian soils.

    PubMed

    Ahmad, Amjad A; Fares, Ali; Abbas, Farhat; Deenik, Jonathan L

    2009-11-01

    The objective of this study was to evaluate the effects of chicken manure (CM) application rates on nutrient concentrations within and below the root zone of sweet corn (Zea mays L. subsp. mays) under Hawaiian conditions. The research was conducted in leeward (Poamoho) and windward (Waimanalo) areas of Oahu, Hawaii, where contrasts exist in both climatic and soil conditions. Suction cup were used to collect soil solutions from 30 and 60 cm depths. Soil solutions were collected six times during the growing season at each location and analyzed for different nutrients (N, P, K, Ca, Mg, Na, Fe, Mn, Zn, and Cu), nitrate-nitrogen (NO(3)-N), ammonium-nitrogen (NH(4)-N), electrical conductivity (EC), and pH. Analysis showed that CM rates significantly affected the concentration of macro-nutrients below the root zone at Poamoho and within the root zone at Waimanalo. In general, nutrient concentration increased with the increasing rates of CM application. There was a significant effect of CM on micro-nutrients except below the root zone at Poamoho. CM significantly affected NO(3)-N concentration within the root zone for 15, 60 days after planting (DAP) at Poamoho, and 16, 28 DAP at Waimanalo. The effect was also significant on total nitrogen (N) concentration in the root zone across the two growing seasons at Waimanalo. There was a highly significant correlation between total N and NO(3)-N, and EC within and below the root zone.

  1. Relating management practices and nutrient export in agricultural watersheds of the United States

    USGS Publications Warehouse

    Sprague, Lori A.; Gronberg, Jo Ann M.

    2012-01-01

    Relations between riverine export (load) of total nitrogen (N) and total phosphorus (P) from 133 large agricultural watersheds in the United States and factors affecting nutrient transport were evaluated using empirical regression models. After controlling for anthropogenic inputs and other landscape factors affecting nutrient transport-such as runoff, precipitation, slope, number of reservoirs, irrigated area, and area with subsurface tile drains-the relations between export and the area in the Conservation Reserve Program (CRP) (N) and conservation tillage (P) were positive. Additional interaction terms indicated that the relations between export and the area in conservation tillage (N) and the CRP (P) progressed from being clearly positive when soil erodibility was low or moderate, to being close to zero when soil erodibility was higher, to possibly being slightly negative only at the 90th to 95th percentile of soil erodibility values. Possible explanations for the increase in nutrient export with increased area in management practices include greater transport of soluble nutrients from areas in conservation tillage; lagged response of stream quality to implementation of management practices because of nitrogen transport in groundwater, time for vegetative cover to mature, and/or prior accumulation of P in soils; or limitations in the management practice and stream monitoring data sets. If lags are occurring, current nutrient export from agricultural watersheds may still be reflecting the influence of agricultural land-use practices that were in place before the implementation of these management practices.

  2. Mathematical modelling of skeletal repair.

    PubMed

    MacArthur, B D; Please, C P; Taylor, M; Oreffo, R O C

    2004-01-23

    Tissue engineering offers significant promise as a viable alternative to current clinical strategies for replacement of damaged tissue as a consequence of disease or trauma. Since mathematical modelling is a valuable tool in the analysis of complex systems, appropriate use of mathematical models has tremendous potential for advancing the understanding of the physical processes involved in such tissue reconstruction. In this review, the potential benefits, and limitations, of theoretical modelling in tissue engineering applications are examined with specific emphasis on tissue engineering of bone. A central tissue engineering approach is the in vivo implantation of a biomimetic scaffold seeded with an appropriate population of stem or progenitor cells. This review will therefore consider the theory behind a number of key factors affecting the success of such a strategy including: stem cell or progenitor population expansion and differentiation ex vivo; cell adhesion and migration, and the effective design of scaffolds; and delivery of nutrient to avascular structures. The focus will be on current work in this area, as well as on highlighting limitations and suggesting possible directions for future work to advance health-care for all.

  3. Chronic binge alcohol consumption during pregnancy alters rat maternal uterine artery pressure response.

    PubMed

    Naik, Vishal D; Lunde-Young, Emilie R; Davis-Anderson, Katie L; Orzabal, Marcus; Ivanov, Ivan; Ramadoss, Jayanth

    2016-11-01

    We aimed to investigate pressure-dependent maternal uterine artery responses and vessel remodeling following gestational binge alcohol exposure. Two groups of pregnant rats were used: the alcohol group (28.5% wt/v, 6.0 g/kg, once-daily orogastric gavage in a binge paradigm between gestational day (GD) 5-19) and pair-fed controls (isocalorically matched). On GD20, excised, pressurized primary uterine arteries were studied following equilibration (60 mm Hg) using dual chamber arteriograph. The uterine artery diameter stabilized at 20 mm Hg, showed passive distension at 40 mm Hg, and redeveloped tone at 60 mm Hg. An alcohol effect (P = 0.0025) was observed on the percent constriction of vessel diameter with greater pressure-dependent myogenic constriction. Similar alcohol effect was noted with lumen diameter response (P = 0.0020). The percent change in media:lumen ratio was higher in the alcohol group (P < 0.0001). Thus, gestational alcohol affects pressure-induced uterine artery reactivity, inward-hypotrophic remodeling, and adaptations critical for nutrient delivery to the fetus. Copyright © 2016 Elsevier Inc. All rights reserved.

  4. System-level analysis of genes and functions affecting survival during nutrient starvation in Saccharomyces cerevisiae.

    PubMed

    Gresham, David; Boer, Viktor M; Caudy, Amy; Ziv, Naomi; Brandt, Nathan J; Storey, John D; Botstein, David

    2011-01-01

    An essential property of all cells is the ability to exit from active cell division and persist in a quiescent state. For single-celled microbes this primarily occurs in response to nutrient deprivation. We studied the genetic requirements for survival of Saccharomyces cerevisiae when starved for either of two nutrients: phosphate or leucine. We measured the survival of nearly all nonessential haploid null yeast mutants in mixed populations using a quantitative sequencing method that estimates the abundance of each mutant on the basis of frequency of unique molecular barcodes. Starvation for phosphate results in a population half-life of 337 hr whereas starvation for leucine results in a half-life of 27.7 hr. To measure survival of individual mutants in each population we developed a statistical framework that accounts for the multiple sources of experimental variation. From the identities of the genes in which mutations strongly affect survival, we identify genetic evidence for several cellular processes affecting survival during nutrient starvation, including autophagy, chromatin remodeling, mRNA processing, and cytoskeleton function. In addition, we found evidence that mitochondrial and peroxisome function is required for survival. Our experimental and analytical methods represent an efficient and quantitative approach to characterizing genetic functions and networks with unprecedented resolution and identified genotype-by-environment interactions that have important implications for interpretation of studies of aging and quiescence in yeast.

  5. Effects of nutrient enrichment on the decomposition of wood and associated microbial activity in streams

    USGS Publications Warehouse

    Gulis, V.; Rosemond, A.D.; Suberkropp, K.; Weyers, H.S.; Benstead, J.P.

    2004-01-01

    1. We determined the effects of nutrient enrichment on wood decomposition rates and microbial activity during a 3-year study in two headwater streams at Coweeta Hydrologic Laboratory, NC, U.S.A. After a 1-year pretreatment period, one of the streams was continuously enriched with inorganic nutrients (nitrogen and phosphorus) for 2 years while the other stream served as a reference. We determined the effects of enrichment on both wood veneers and sticks, which have similar carbon quality but differ in physical characteristics (e.g. surface area to volume ratios, presence of bark) that potentially affect microbial colonisation and activity. 2. Oak wood veneers (0.5 mm thick) were placed in streams monthly and allowed to decompose for approximately 90 days. Nutrient addition stimulated ash-free dry mass loss and increased mean nitrogen content, fungal biomass and microbial respiration on veneers in the treatment stream compared with the reference. The magnitude of the response to enrichment was great, with mass loss 6.1 times, and per cent N, fungal biomass and microbial respiration approximately four times greater in the treatment versus reference stream. 3. Decomposition rate and nitrogen content of maple sticks (ca. 1-2 cm diameter) also increased; however, the effect was less pronounced than for veneers. Wood response overall was greater than that determined for leaves in a comparable study, supporting the hypothesis that response to enrichment may be greater for lower quality organic matter (high C:N) than for higher quality (low C:N) substrates. 4. Our results show that moderate nutrient enrichment can profoundly affect decomposition rate and microbial activity on wood in streams. Thus, the timing and availability of wood that provides retention, structure, attachment sites and food in stream ecosystems may be affected by nutrient concentrations raised by human activities.

  6. Estimating the sources and transport of nutrients in the Waikato River Basin, New Zealand

    USGS Publications Warehouse

    Alexander, Richard B.; Elliott, Alexander H.; Shankar, Ude; McBride, Graham B.

    2002-01-01

    We calibrated SPARROW (Spatially Referenced Regression on Watershed Attributes) surface water‐quality models using measurements of total nitrogen and total phosphorus from 37 sites in the 13,900‐km2 Waikato River Basin, the largest watershed on the North Island of New Zealand. This first application of SPARROW outside of the United States included watersheds representative of a wide range of natural and cultural conditions and water‐resources data that were well suited for calibrating and validating the models. We applied the spatially distributed model to a drainage network of nearly 5000 stream reaches and 75 lakes and reservoirs to empirically estimate the rates of nutrient delivery (and their levels of uncertainty) from point and diffuse sources to streams, lakes, and watershed outlets. The resulting models displayed relatively small errors; predictions of stream yield (kg ha−1 yr−1) were typically within 30% or less of the observed values at the monitoring sites. There was strong evidence of the accuracy of the model estimates of nutrient sources and the natural rates of nutrient attenuation in surface waters. Estimated loss rates for streams, lakes, and reservoirs agreed closely with experimental measurements and empirical models from New Zealand, North America, and Europe as well as with previous U.S. SPARROW models. The results indicate that the SPARROW modeling technique provides a reliable method for relating experimental data and observations from small catchments to the transport of nutrients in the surface waters of large river basins.

  7. Sources and Delivery of Nutrients to the Northwestern Gulf of Mexico from Streams in the South-Central United States

    USGS Publications Warehouse

    Rebich, R.A.; Houston, N.A.; Mize, S.V.; Pearson, D.K.; Ging, P.B.; Evan, Hornig C.

    2011-01-01

    SPAtially Referenced Regressions On Watershed attributes (SPARROW) models were developed to estimate nutrient inputs [total nitrogen (TN) and total phosphorus (TP)] to the northwestern part of the Gulf of Mexico from streams in the South-Central United States (U.S.). This area included drainages of the Lower Mississippi, Arkansas-White-Red, and Texas-Gulf hydrologic regions. The models were standardized to reflect nutrient sources and stream conditions during 2002. Model predictions of nutrient loads (mass per time) and yields (mass per area per time) generally were greatest in streams in the eastern part of the region and along reaches near the Texas and Louisiana shoreline. The Mississippi River and Atchafalaya River watersheds, which drain nearly two-thirds of the conterminous U.S., delivered the largest nutrient loads to the Gulf of Mexico, as expected. However, the three largest delivered TN yields were from the Trinity River/Galveston Bay, Calcasieu River, and Aransas River watersheds, while the three largest delivered TP yields were from the Calcasieu River, Mermentau River, and Trinity River/Galveston Bay watersheds. Model output indicated that the three largest sources of nitrogen from the region were atmospheric deposition (42%), commercial fertilizer (20%), and livestock manure (unconfined, 17%). The three largest sources of phosphorus were commercial fertilizer (28%), urban runoff (23%), and livestock manure (confined and unconfined, 23%). ?? 2011 American Water Resources Association. This article is a U.S. Government work and is in the public domain in the USA.

  8. Influence of key processing parameters and seeding density effects of microencapsulated chondrocytes fabricated using electrohydrodynamic spraying.

    PubMed

    Gansau, Jennifer; Kelly, Lara; Buckley, Conor

    2018-06-11

    Cell delivery and leakage during injection remains a challenge for cell-based intervertebral disc regeneration strategies. Cellular microencapsulation may offer a promising approach to overcome these limitations by providing a protective niche during intradiscal injection. Electrohydrodynamic spraying (EHDS) is a versatile one-step approach for microencapsulation of cells using a high voltage electric field. The primary objective of this work was to characterise key processing parameters such as applied voltage (0, 5, 10 or 15kV), emitter needle gauge (21, 26 or 30G), alginate concentration (1, 2 or 3%) and flow rate (50, 100, 250 or 500 µl/min) to regulate the morphology of alginate microcapsules and subsequent cell viability when altering these parameters. The effect of initial cell seeding density (5, 10 and 20x10<sup>6</sup> cells/ml) on subsequent matrix accumulation of microencapsulated articular chondrocytes was also evaluated. Results showed that increasing alginate concentration and thus viscosity increased overall microcapsule size but also affected the geometry towards ellipsoidal-shaped gels. Altering the electric field strength and needle diameter regulated microcapsule size towards a smaller diameter with increasing voltage and smaller needle diameter. Needle size did not appear to affect cell viability when operating with lower alginate concentrations (1% and 2%), although higher concentrations (3%) and thus higher viscosity hydrogels resulted in diminished viability with decreasing needle diameter. Increasing cell density resulted in decreased cell viability and a concomitant decrease in DNA content, perhaps due to competing nutrient demands as a result of more closely packed cells. However, higher cell densities resulted in increased levels of extracellular matrix accumulated. Overall, this work highlights the potential of EHDS as a controllable and versatile approach to fabricate microcapsules for injectable delivery which can be used in a variety of applications such as drug development or cell therapies. . © 2018 IOP Publishing Ltd.

  9. Impact of hazardous events on the removal of nutrients and trace organic contaminants by an anoxic-aerobic membrane bioreactor receiving real wastewater.

    PubMed

    Phan, Hop V; Hai, Faisal I; McDonald, James A; Khan, Stuart J; van de Merwe, Jason P; Leusch, Frederic D L; Zhang, Ren; Price, William E; Broeckmann, Andreas; Nghiem, Long D

    2015-09-01

    The impacts of four simulated hazardous events, namely, aeration failure, power loss, and chemical shocks (ammonia or bleach) on the performance of an anoxic-aerobic membrane bioreactor (MBR) receiving real wastewater were investigated. Hazardous events could alter pH and/or oxidation reduction potential of the mixed liquor and inhibit biomass growth, thus affecting the removal of bulk organics, nutrients and trace organic contaminants (TrOC). Chemical shocks generally exerted greater impact on MBR performance than aeration/power failure events, with ammonia shock exerting the greatest impact. Compared to total organic carbon, nutrient removal was more severely affected. Removal of the hydrophilic TrOCs that are resistant and/or occur at high concentrations in wastewater was notably affected. The MBR effectively reduced estrogenicity and toxicity from wastewater, but chemical shocks could temporarily increase the endocrine activity of the effluent. Depending on the chemical shock-dose and the membrane flux, hazardous events can exacerbate membrane fouling. Copyright © 2015 Elsevier Ltd. All rights reserved.

  10. Environmental Stress-mediated EPS Production Shape Microbial Activity on Various Hydrated Rough Surfaces

    NASA Astrophysics Data System (ADS)

    Wang, G.; Liu, L.; Chen, G.

    2016-12-01

    The complex environmental physical and chemical processes and interplay with the associating biological responses are keys to understanding the environmental microbiology ensconced in environmental remediation, water quality control, food safety, nutrient cycling, and etc., yet remain poorly understood. Using experimental micromodels, we study how environmental conditions (e.g., hydration fluctuation, nutrient limitation, pH variation, etc.) affect microbial extracellular polymeric substances (EPS) production and their configuration within various hydrated surfaces, and impacts on microbial motility, surface attachment, aggregation, and other bioremediation activities. To elucidate the potential mechanisms underlying the complex bio-physicochemical processes, we developed an individual-based and spatio-temporally resolved modeling platform that explicitly considers microscale aqueous-phase configuration and nutrient transport/diffusion and associated biophysical processes affecting individual microbial cell life history. We quantitatively explore the effects of the above microscale environmental processes on bio-physicochemical interactions affecting microbial growth, motility, surface attachment and aggregation, and shaping population interactions and functions. Simulation scenarios of microbial induced pollutant (e.g., roxarsone) biotransformation on various hydrated rough surfaces will also be present.

  11. Interactive effects of compost and pre-planting soil moisture on plant biomass, nutrition and formation of mycorrhizas: a context dependent response.

    PubMed

    Ngo, H T T; Cavagnaro, T R

    2018-01-24

    We aimed to investigate the combined impacts of compost addition and pre-planting soil moisture conditions, on plant-available nutrients, and subsequent impacts on the biomass, nutrition and formation of AM by two important crop species. A glasshouse study was undertaken in which wheat and tomato plants were grown in compost amended or un-amended soil that was subjected to different moisture regimes prior to planting. The availability of P was strongly influenced by compost addition, but not pre-planting moisture conditions. In contrast, mineral N pools were affected by compost addition and pre-planting soil moisture conditions in complex ways. These changes in nutrient availability affected plant biomass, nutrient uptake and formation of AM. In general, plant performance was better where pre-planting soil moisture conditions were wet or dry, and worse where they involved a wet/dry cycle, and mycorrhizal colonisation was lower where compost was added to the soil. That pre-planting moisture conditions affect the biomass of subsequent crops is an important finding, the potential implications of which are considered here.

  12. Inclusion of phytogenic blends in different nutrient density diets of meat-type ducks.

    PubMed

    Gheisar, Mohsen Mohammadi; Im, Yong Woon; Lee, Hae Hyoung; Choi, Yang Il; Kim, In Ho

    2015-12-01

    A total of 160 1-day-old ducklings (average initial body weight of 53 g), were used in a 42-d feeding trial to evaluate the effects of reducing nutrient density of diets, and supplementing the diets with a phytogenic blend (quillaja, anise, and thyme) on their growth, carcass quality, and nutrient digestibility. After checking body weight on d 1, the birds were sorted into pens with 5 birds/pen and 8 pens/treatment. The treatments were: T1, Basal diet; T2, T1+150 ppm phytogenic blend; T3, T1-(1% CP, 0.04% Lys, 0.05% Met+Cys, 0.02% Ca, and 0.02% P, and 50 kcal ME); T4, T3+150 ppm phytogenic blend. The results indicated that reducing nutrient density of the diets had an adverse effect (P<0.05) on body weight gain (BWG) on d 1 to 21, d 21 to 42, and the overall experimental period. Supplementing the diets with the phytogenic blend improved (P<0.05) BWG and feed conversion ratio (FCR) on d 21 to 42 and the overall experimental period. Feed intake was not affected by treatments. Low nutrient density diets increased (P<0.05) the cooking loss percentage of breast meat. Supplementing the diets with the phytogenic blend decreased (P<0.05) the lightness of breast meat. The percentage of drip loss was influenced (P<0.05) by nutrient density and the phytogenic blend on d 1 and d 7. The relative weights of breast meat, abdominal fat, gizzard, liver, spleen, and bursa of Fabricius, pH, and TBARS values were not affected by the treatments. The digestibility of dry matter, energy, nitrogen, ADF, and NDF was decreased (P<0.05) by reducing nutrients density of the diets, but addition of the phytogenic blend alleviated (P<0.05) the negative effects of lowering the nutrient density. The results indicated that the ducks fed high nutrient density diets supplemented with the phytogenic blend showed higher BWG and nutrient digestibility and lower FCR, cooking loss, drip loss, and TBARS value, without any negative effect on meat quality and relative organs weights. © 2015 Poultry Science Association Inc.

  13. Linking environmental nutrient enrichment and disease emergence in humans and wildlife

    PubMed Central

    Johnson, Pieter T. J.; Townsend, Alan R.; Cleveland, Cory C.; Glibert, Patricia M.; Howarth, Robert W.; McKenzie, Valerie J.; Rejmankova, Eliska; Ward, Mary H.

    2009-01-01

    Worldwide increases in the numbers of human and wildlife diseases present ecologists with the challenge of understanding how large-scale environmental changes affect host-parasite interactions. One of the most profound changes to Earth’s ecosystems is the alteration of global nutrient cycles, including those of phosphorus (P) and especially nitrogen (N). Alongside the obvious direct benefits of nutrient application for food production, growing evidence suggests that anthropogenic inputs of N and P can indirectly affect the abundance of infectious and noninfectious pathogens, sometimes leading to epidemic conditions. However, the mechanisms underpinning observed correlations, and how such patterns vary with disease type, have long remained conjectural. Here, we discuss recent experimental advances in this area to critically evaluate the relationship between environmental nutrient enrichment and disease. Given the inter-related nature of human and wildlife disease emergence, we include a broad range of human and wildlife examples from terrestrial, marine and freshwater ecosystems. We examine the consequences of nutrient pollution on directly transmitted, vector-borne, complex life cycle, and noninfectious pathogens, including West Nile virus, malaria, harmful algal blooms, coral reef diseases and amphibian malformations. Our synthetic examination suggests that the effects of environmental nutrient enrichment on disease are complex and multifaceted, varying with the type of pathogen, host species and condition, attributes of the ecosystem and the degree of enrichment; some pathogens increase in abundance whereas others decline or disappear. Nevertheless, available evidence indicates that ecological changes associated with nutrient enrichment often exacerbate infection and disease caused by generalist parasites with direct or simple life cycles. Observed mechanisms include changes in host/vector density, host distribution, infection resistance, pathogen virulence or toxicity, or the direct supplementation of pathogens. Collectively, these pathogens may be particularly dangerous because they can continue to cause mortality even as their hosts decline, potentially leading to sustained epidemics or chronic pathology. We suggest that interactions between nutrient enrichment and disease will become increasingly important in tropical and subtropical regions, where forecasted increases in nutrient application will occur in an environment rich with infectious pathogens. We emphasize the importance of careful disease management in conjunction with continued intensification of global nutrient cycles. PMID:20349828

  14. Nutrient Intake of Dengue Hemorrhagic Fever Patients in Semarang City

    NASA Astrophysics Data System (ADS)

    Ratri Maharani, Agustina; Restuti, Christina Tri; Sari, Erna; Endah Wahyuningsih, Nur; Murwani, Retno; Hapsari, MMDEAH

    2018-05-01

    Dengue Hemorrhagic Fever (DHF) is an acute infectious disease caused by dengue virus and transmission of the virus is mediated by mosquitoes bites [1]. Host immunity against dengue infection is affected by nutrient adequacy which is depending on nutrient intake [2]. The aim of this study was to determine nutrient intake of DHF patients in Semarang city. The DHF sample cases were obtained from three hospitals in Semarang city (n=48), from the period of March to May 2016 and the control groups were obtained from healthy respondents with matched age, sex, and district location (n=48). Nutrient intake were obtained by food recall and calculated using Nutrisurvey Indonesia. Afterwards, the result of the nutrisurvey will be compared to Indonesian daily value according to Permenkes no. 75 about daily value based on age and gender. The results showed that both in cases and control groups the macro-(energy, carbohydrate, protein, fat) and micro-nutrient (vitamins A., C, B1, B2, B6, calcium, magnesium, phosphorus, zinc, and Iron) intake were below 80% of nutrient adequacy. No correlation was found between nutrient adequacy and DHF cases. We find that macro and micronutrient intake in DHF case and control groups are the same and below 80% of nutrient adequacy. The nutrient intake was not related to DHF cases.

  15. Cation Uptake and Allocation by Red Pine Seedlings under Cation-Nutrient Stress in a Column Growth Experiment

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Shi, Zhenqing; Balogh-Brunstad, Zsuzsanna; Grant, Michael R.

    Background and Aims Plant nutrient uptake is affected by environmental stress, but how plants respond to cation-nutrient stress is poorly understood. We assessed the impact of varying degrees of cation-nutrient limitation on cation uptake in an experimental plant-mineral system. Methods Column experiments, with red pine (Pinus resinosa Ait.) seedlings growing in sand/mineral mixtures, were conducted for up to nine months under a range of Ca- and K-limited conditions. The Ca and K were supplied from both minerals and nutrient solutions with varying Ca and K concentrations. Results Cation nutrient stress had little impact on carbon allocation after nine months ofmore » plant growth and K was the limiting nutrient for biomass production. The Ca/Sr and K/Rb ratio results allowed independent estimation of dissolution incongruency and discrimination against Sr and Rb during cation uptake processes. The fraction of K in biomass from biotite increased with decreasing K supply from nutrient solutions. The mineral anorthite was consistently the major source of Ca, regardless of nutrient treatment. Conclusions Red pine seedlings exploited more mineral K in response to more severe K deficiency. This did not occur for Ca. Plant discrimination factors must be carefully considered to accurately identify nutrient sources using cation tracers.« less

  16. Drug-nutrient interactions.

    PubMed

    Chan, Lingtak-Neander

    2013-07-01

    Drug-nutrient interactions are defined as physical, chemical, physiologic, or pathophysiologic relationships between a drug and a nutrient. The causes of most clinically significant drug-nutrient interactions are usually multifactorial. Failure to identify and properly manage drug-nutrient interactions can lead to very serious consequences and have a negative impact on patient outcomes. Nevertheless, with thorough review and assessment of the patient's history and treatment regimens and a carefully executed management strategy, adverse events associated with drug-nutrient interactions can be prevented. Based on the physiologic sequence of events after a drug or a nutrient has entered the body and the mechanism of interactions, drug-nutrient interactions can be categorized into 4 main types. Each type of interaction can be managed using similar strategies. The existing data that guide the clinical management of most drug-nutrient interactions are mostly anecdotal experience, uncontrolled observations, and opinions, whereas the science in understanding the mechanism of drug-nutrient interactions remains limited. The challenge for researchers and clinicians is to increase both basic and higher level clinical research in this field to bridge the gap between the science and practice. The research should aim to establish a better understanding of the function, regulation, and substrate specificity of the nutrient-related enzymes and transport proteins present in the gastrointestinal tract, as well as assess how the incidence and management of drug-nutrient interactions can be affected by sex, ethnicity, environmental factors, and genetic polymorphisms. This knowledge can help us develop a true personalized medicine approach in the prevention and management of drug-nutrient interactions.

  17. Fragile and conflict affected states: report from the Consultation on Collaboration for Applied Health Research and Delivery

    PubMed Central

    2014-01-01

    Fragile and Conflict Affected States present difficult contexts to achieve health system outcomes and are neglected in health systems research. This report presents key debates from the Consultation of the Collaboration for Applied Health Research and Delivery, Liverpool, June, 2014.

  18. Mixing of water masses caused by a drifting iceberg affects bacterial activity, community composition and substrate utilization capability in the Southern Ocean.

    PubMed

    Dinasquet, Julie; Richert, Inga; Logares, Ramiro; Yager, Patricia; Bertilsson, Stefan; Riemann, Lasse

    2017-06-01

    The number of icebergs produced from ice-shelf disintegration has increased over the past decade in Antarctica. These drifting icebergs mix the water column, influence stratification and nutrient condition, and can affect local productivity and food web composition. Data on whether icebergs affect bacterioplankton function and composition are scarce, however. We assessed the influence of iceberg drift on bacterial community composition and on their ability to exploit carbon substrates during summer in the coastal Southern Ocean. An elevated bacterial production and a different community composition were observed in iceberg-influenced waters relative to the undisturbed water column nearby. These major differences were confirmed in short-term incubations with bromodeoxyuridine followed by CARD-FISH. Furthermore, one-week bottle incubations amended with inorganic nutrients and carbon substrates (a mix of substrates, glutamine, N-acetylglucosamine, or pyruvate) revealed contrasting capacity of bacterioplankton to utilize specific carbon substrates in the iceberg-influenced waters compared with the undisturbed site. Our study demonstrates that the hydrographical perturbations introduced by a drifting iceberg can affect activity, composition, and substrate utilization capability of marine bacterioplankton. Consequently, in a context of global warming, increased frequency of drifting icebergs in polar regions holds the potential to affect carbon and nutrient biogeochemistry at local and possibly regional scales. © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.

  19. The optimal mode of delivery for the haemophilia carrier expecting an affected infant is vaginal delivery.

    PubMed

    Ljung, R

    2010-05-01

    The optimal mode of delivery of a haemophilia carrier expecting a child is still a matter of uncertainty and debate. The aim of this commentary/review is to suggest that normal vaginal delivery should be the recommended mode of delivery for the majority of carriers, based on review of studies on obstetric aspects of haemophilia. About 2.0-4.0% of all haemophilia boys born in countries with a good standard of health care will suffer from ICH during the neonatal period. This is an average figure including all modes of delivery and regardless of whether the carrier status of the mother or the haemophilia status of the foetus was known or not at the time of delivery. On the basis of current literature, one may conclude that the risk of serious bleeding in the neonate affected with haemophilia is small in conjunction with normal vaginal delivery. It should be possible to further reduce the low frequency of complications if appropriate precautions are taken when planning the delivery in pregnant woman with known carrier status, if the sex of the foetus is known and, even more, when the haemophilia status of the foetus is known. Instrumental delivery such as use of vacuum extraction and foetal scalp monitors must be avoided at delivery of carriers.

  20. Role of model structure on the response of soil biogeochemistry to hydro-climatic fluctuations

    NASA Astrophysics Data System (ADS)

    Manzoni, S.; Porporato, A.

    2005-05-01

    Soil carbon and nutrient cycles are strongly affected by hydro-climatic variability, which interacts with the internal ecosystem structure. Here we test the implications of biogeochemical model structure on such dynamics by extending an existing model by the authors and coworkers. When forced by hydro-climatic fluctuations, the different model structures induce specific preferential nutrient paths among the soil pools, which in turn affect nutrient distribution and availability to microbes and plants. In particular, if it is assumed that microbes can directly assimilate organic nitrogen, plants tend to be inferior competitors for nutrients even in well-watered conditions, while if a certain amount of organic nitrogen is assumed to be mineralized without being first incorporated into microbial cells, vegetation can be advantaged over a wide range of soil moisture values. We also investigate the intensification of competition for nutrients (e.g., nitrogen) between plant and soil microbial communities under extreme hydrologic conditions, such as droughts and intense storms. Frequent rainfall events may determine ideal soil moisture conditions for plant uptake, enhancing nitrogen leaching while lowering oxygen concentration and inhibiting microbial activity. During droughts, the soil water potential often drops to the point of hampering the plant nutrient uptake while still remaining high enough for microbial decomposition and nitrogen immobilization. The interplay of microbe and vegetation water stress is investigated in depth as it controls the ability of one community (e.g., plants or soil microbes) to establish competitive advantage on the other. The long-term effects of these dynamics of competition and nutrient allocation are explored under steady-state and stochastic soil moisture conditions to analyze the feedbacks between soil organic matter and vegetation dynamics.

  1. Effects of herbivore exclusion and nutrient enrichment on coral reef macroalgae and cyanobacteria

    NASA Astrophysics Data System (ADS)

    Thacker, R.; Ginsburg, D.; Paul, V.

    2001-05-01

    Although phase shifts on coral reefs from coral-dominated to algal-dominated communities have been attributed to the effects of increased nutrient availability due to eutrophication and reduced herbivore abundance due to overfishing and disease, these factors have rarely been manipulated simultaneously. In addition, few studies have considered the effects of these factors on benthic, filamentous cyanobacteria (blue-green algae) as well as macroalgae. We used a combination of herbivore-exclusion cages and nutrient enrichment to manipulate herbivore abundance and nutrient availability, and measured the impacts of these treatments on macroalgal and cyanobacterial community structure. In the absence of cages, surface cover of the cyanobacterium Tolypothrix sp. decreased, while surface cover of the cyanobacteria Oscillatoria spp. increased. Cyanobacterial cover decreased in partial cages, and Tolypothrix sp. cover decreased further in full cages. Lower cyanobacterial cover and biomass were correlated with higher macroalgal cover and biomass. Dictyota bartayresiana dominated the partial cages, while Padina tenuis and Tolypiocladia glomerulata recruited into the full cages. Palatability assays demonstrated that herbivore-exclusion shifted macroalgal species composition from relatively unpalatable to relatively palatable species. Nutrient enrichment interacted with herbivore exclusion to increase the change in cover of D. bartayresiana in the uncaged and fully caged plots, but did not affect the final biomass of D. bartayresiana among treatments. Nutrient enrichment did not significantly affect the cover or biomass of any other taxa. These results stress the critical role of herbivory in determining coral reef community structure and suggest that the relative palatabilities of dominant algae, as well as algal growth responses to nutrient enrichment, will determine the potential for phase shifts to algal-dominated communities.

  2. Effect of Temperature and Nutrient Concentration on Survival of Foodborne Pathogens in Deciduous Fruit Processing Environments for Effective Hygiene Management.

    PubMed

    Duvenage, Stacey; Korsten, Lise

    2016-11-01

    Temperature and good sanitation practices are important factors for controlling growth of microorganisms. Fresh produce is stored at various temperatures to ensure quality and to prolong shelf life. When foodborne pathogens survive and grow on fresh produce at storage temperatures, then additional control strategies are needed to inactivate these pathogens. The aim of this study was to determine how temperatures associated with deciduous fruit processing and storage facilities (0.5, 4, and 21°C) affect the growth and/or survival of Escherichia coli O157:H7, Listeria monocytogenes , Salmonella enterica subsp. enterica serovar Typhimurium, and Staphylococcus aureus under different nutrient conditions (nutrient rich and nutrient poor) and on simulated contact surfaces (vinyl coupons). Information on the growth and survival of foodborne pathogens at specific deciduous fruit processing and storage temperatures (0.5°C) is not available. All pathogens except E. coli O157:H7 were able to survive on vinyl coupons at all temperatures. L. monocytogenes proliferated under both nutrient conditions independent of temperature. S. aureus was the pathogen least affected by nutrient conditions. The survival of foodborne pathogens on the vinyl coupons, a model system for studying surfaces in fruit preparation and storage environments, indicates the potential for cross-contamination of deciduous fruit products under poor sanitation conditions. Foodborne pathogens that can proliferate and survive at various temperatures under different nutrient conditions could lead to fruit cross-contamination. Temperature mismanagement, which could allow pathogen proliferation in contaminated fruit packing houses and storage environments, is a concern. Therefore, proper hygiene and sanitation practices, removal of possible contaminants, and proper food safety management systems are needed to ensure food safety.

  3. Effects of temperature, algae biomass and ambient nutrient on the absorption of dissolved nitrogen and phosphate by Rhodophyte Gracilaria asiatica

    NASA Astrophysics Data System (ADS)

    Du, Rongbin; Liu, Liming; Wang, Aimin; Wang, Yongqiang

    2013-03-01

    Gracilaria asiatica, being highly efficient in nutrient absorption, is cultivated in sea cucumber ponds to remove nutrients such as nitrogen and phosphate. It was cultured in a laboratory simulating field conditions, and its nutrient absorption was measured to evaluate effects of environmental conditions. Ammonia nitrogen (AN), nitrate nitrogen (NN), total inorganic nitrogen (TIN), and soluble reactive phosphorus (SRP) uptake rate and removal efficiency were determined in a 4×2 factorial design experiment in water temperatures ( T) at 15°C and 25°C, algae biomass (AB) at 0.5 g/L and 1.0 g/L, total inorganic nitrogen (TIN) at 30 μmol/L and 60 μmol/L, and soluble reactive phosphorus (SRP) at 3 and 6 μmol/L. AB and ambient TIN or SRP levels significantly affected uptake rate and removal efficiency of AN, NN, TIN, and SRP ( P< 0.001). G. asiatica in AB of 0.5 g/L showed higher uptake rate and lower removal efficiency relative to that with AB of 1.0 g/L. Nitrogen and phosphorus uptake rate rose with increasing ambient nutrient concentrations; nutrient removal efficiency decreased at higher environmental nutrient concentrations. The algae preferred to absorb AN to NN. Uptake rates of AN, NN, and SRP were significantly affected by temperature ( P < 0.001); uptake rate was higher for the 25°C group than for the 15°C group at the initial experiment stage. Only the removal efficiency of AN and SRP showed a significant difference between the two temperature groups ( P< 0.01). The four factors had significant interactive effects on absorption of N and P, implying that G. asiatica has great bioremedial potential in sea cucumber culture ponds.

  4. Cell-specific CO2 fixation rates of two distinct groups of plastidic protists in the Atlantic Ocean remain unchanged after nutrient addition.

    PubMed

    Grob, Carolina; Jardillier, Ludwig; Hartmann, Manuela; Ostrowski, Martin; Zubkov, Mikhail V; Scanlan, David J

    2015-04-01

    To assess the role of open-ocean ecosystems in global CO2 fixation, we investigated how picophytoplankton, which dominate primary production, responded to episodic increases in nutrient availability. Previous experiments have shown nitrogen alone, or in combination with phosphorus or iron, to be the proximate limiting nutrient(s) for total phytoplankton grown over several days. Much less is known about how nutrient upshift affects picophytoplankton CO2 fixation over the duration of the light period. To address this issue, we performed a series of small volume (8-60 ml) - short term (10-11 h) nutrient addition experiments in different regions of the Atlantic Ocean using NH4 Cl, FeCl3 , K medium, dust and nutrient-rich water from 300 m depth. We found no significant nutrient stimulation of group-specific CO2 fixation rates of two taxonomically and size-distinct groups of plastidic protists. The above was true regardless of the region sampled or nutrient added, suggesting that this is a generic phenomenon. Our findings show that at least in the short term (i.e. daylight period), nutrient availability does not limit CO2 fixation by the smallest plastidic protists, while their taxonomic composition does not determine their response to nutrient addition. © 2014 Society for Applied Microbiology and John Wiley & Sons Ltd.

  5. A Dietary Feedback System for the Delivery of Consistent Personalized Dietary Advice in the Web-Based Multicenter Food4Me Study

    PubMed Central

    Forster, Hannah; Walsh, Marianne C; O'Donovan, Clare B; Woolhead, Clara; McGirr, Caroline; Daly, E.J; O'Riordan, Richard; Celis-Morales, Carlos; Fallaize, Rosalind; Macready, Anna L; Marsaux, Cyril F M; Navas-Carretero, Santiago; San-Cristobal, Rodrigo; Kolossa, Silvia; Hartwig, Kai; Mavrogianni, Christina; Tsirigoti, Lydia; Lambrinou, Christina P; Godlewska, Magdalena; Surwiłło, Agnieszka; Gjelstad, Ingrid Merethe Fange; Drevon, Christian A; Manios, Yannis; Traczyk, Iwona; Martinez, J Alfredo; Saris, Wim H M; Daniel, Hannelore; Lovegrove, Julie A; Mathers, John C; Gibney, Michael J; Gibney, Eileen R

    2016-01-01

    Background Despite numerous healthy eating campaigns, the prevalence of diets high in saturated fatty acids, sugar, and salt and low in fiber, fruit, and vegetables remains high. With more people than ever accessing the Internet, Web-based dietary assessment instruments have the potential to promote healthier dietary behaviors via personalized dietary advice. Objective The objectives of this study were to develop a dietary feedback system for the delivery of consistent personalized dietary advice in a multicenter study and to examine the impact of automating the advice system. Methods The development of the dietary feedback system included 4 components: (1) designing a system for categorizing nutritional intakes; (2) creating a method for prioritizing 3 nutrient-related goals for subsequent targeted dietary advice; (3) constructing decision tree algorithms linking data on nutritional intake to feedback messages; and (4) developing personal feedback reports. The system was used manually by researchers to provide personalized nutrition advice based on dietary assessment to 369 participants during the Food4Me randomized controlled trial, with an automated version developed on completion of the study. Results Saturated fatty acid, salt, and dietary fiber were most frequently selected as nutrient-related goals across the 7 centers. Average agreement between the manual and automated systems, in selecting 3 nutrient-related goals for personalized dietary advice across the centers, was highest for nutrient-related goals 1 and 2 and lower for goal 3, averaging at 92%, 87%, and 63%, respectively. Complete agreement between the 2 systems for feedback advice message selection averaged at 87% across the centers. Conclusions The dietary feedback system was used to deliver personalized dietary advice within a multi-country study. Overall, there was good agreement between the manual and automated feedback systems, giving promise to the use of automated systems for personalizing dietary advice. Trial Registration Clinicaltrials.gov NCT01530139; https://clinicaltrials.gov/ct2/show/NCT01530139 (Archived by WebCite at http://www.webcitation.org/6ht5Dgj8I) PMID:27363307

  6. Sediment and nutrient delivery from thermokarst features in the foothills of the North Slope, Alaska: Potential impacts on headwater stream ecosystems

    USGS Publications Warehouse

    Bowden, W.B.; Gooseff, M.N.; Balser, A.; Green, A.; Peterson, B.J.; Bradford, J.

    2008-01-01

    Permafrost is a defining characteristic of the Arctic environment. However, climate warming is thawing permafrost in many areas leading to failures in soil structure called thermokarst. An extensive survey of a 600 km2 area in and around the Toolik Lake Natural Research Area (TLNRA) revealed at least 34 thermokarst features, two thirds of which were new since ???1980 when a high resolution aerial survey of the area was done. Most of these thermokarst features were associated with headwater streams or lakes. We have measured significantly increased sediment and nutrient loading from thermokarst features to streams in two well-studied locations near the TLNRA. One small thermokarst gully that formed in 2003 on the Toolik River in a 0.9 km2 subcatchment delivered more sediment to the river than is normally delivered in 18 years from 132 km2 in the adjacent upper Kuparuk River basin (a long-term monitoring reference site). Ammonium, nitrate, and phosphate concentrations downstream from a thermokarst feature on Imnavait Creek increased significantly compared to upstream reference concentrations and the increased concentrations persisted over the period of sampling (1999-2005). The downstream concentrations were similar to those we have used in a long-term experimental manipulation of the Kuparuk River and that have significantly altered the structure and function of that river. A subsampling of other thermokarst features from the extensive regional survey showed that concentrations of ammonium, nitrate, and phosphate were always higher downstream of the thermokarst features. Our previous research has shown that even minor increases in nutrient loading stimulate primary and secondary production. However, increased sediment loading could interfere with benthic communities and change the responses to increased nutrient delivery. Although the terrestrial area impacted by thermokarsts is limited, the aquatic habitat altered by these failures can be extensive. If warming in the Arctic foothills accelerates thermokarst formation, there may be substantial and wide-spread impacts on arctic stream ecosystems that are currently poorly understood. Copyright 2008 by the American Geophysical Union.

  7. Factors affecting the growth of Didymosphenia geminata in New Zealand rivers: Flow, bed disturbance, nutrients, light, and seasonal dynamics. (Invited)

    NASA Astrophysics Data System (ADS)

    Cullis, J. D.; Gillis, C.; Drummond, J. D.; Garcia, T.; Kilroy, C.; Larned, S.; Hassan, M. A.

    2010-12-01

    Didymosphenia geminata (didymo) was introduced into a New Zealand river in 2004, and since then has dramatically spread to cover the beds of many rivers with extremely dense and extensive mats. Successful management is hampered by the fact that much is still unknown about the factors affecting the growth of this nuisance species. We synthesized available data on the distribution of D. geminata in New Zealand rivers to determine how physical and chemical system conditions (flow, bed disturbance, nutrients, and light) affect the growth and persistence of this organism. Here we assess results from bi-weekly surveys performed over a full year on two rivers where didymo was first observed in New Zealand; the Oreti and Mararoa. We used the data to test the hypotheses that the development of thick, dense mats requires high light levels but is inversely proportional to nutrient levels, and that mat persistence is controlled by the frequency of flow events that produce bed sediment transport. Observed regrowth between disturbance events was found to be inversely correlated with nutrient availability. The seasonal availability of light did not correlate with variations in growth rate, but this did not account for specific characteristics of the different sites such as aspect, shading, flow depth and turbidity that will all impact on the amount of available light reaching the streambed. The results clearly indicate that the time-history of flow and nutrient levels is critical to evaluating the growth and persistence of D. geminata and that additional site specific information is necessary to determine the role of bed stability and the amount of available light reaching the streambed.

  8. Evolving Nutritional Strategies in the Presence of Competition: A Geometric Agent-Based Model

    PubMed Central

    Senior, Alistair M.; Charleston, Michael A.; Lihoreau, Mathieu; Buhl, Jerome; Raubenheimer, David; Simpson, Stephen J.

    2015-01-01

    Access to nutrients is a key factor governing development, reproduction and ultimately fitness. Within social groups, contest-competition can fundamentally affect nutrient access, potentially leading to reproductive asymmetry among individuals. Previously, agent-based models have been combined with the Geometric Framework of nutrition to provide insight into how nutrition and social interactions affect one another. Here, we expand this modelling approach by incorporating evolutionary algorithms to explore how contest-competition over nutrient acquisition might affect the evolution of animal nutritional strategies. Specifically, we model tolerance of nutrient excesses and deficits when ingesting nutritionally imbalanced foods, which we term ‘nutritional latitude’; a higher degree of nutritional latitude constitutes a higher tolerance of nutritional excess and deficit. Our results indicate that a transition between two alternative strategies occurs at moderate to high levels of competition. When competition is low, individuals display a low level of nutritional latitude and regularly switch foods in search of an optimum. When food is scarce and contest-competition is intense, high nutritional latitude appears optimal, and individuals continue to consume an imbalanced food for longer periods before attempting to switch to an alternative. However, the relative balance of nutrients within available foods also strongly influences at what levels of competition, if any, transitions between these two strategies occur. Our models imply that competition combined with reproductive skew in social groups can play a role in the evolution of diet breadth. We discuss how the integration of agent-based, nutritional and evolutionary modelling may be applied in future studies to further understand the evolution of nutritional strategies across social and ecological contexts. PMID:25815976

  9. Transformations of Heavy Metals and Plant Nutrients in Dredged Sediments as Affected by Oxidation Reduction Potential and pH. Volume 2. Materials and Methods/Results and Discussion

    DTIC Science & Technology

    1977-05-01

    D )EGED IATWRAL WI0, 00 EEACHPRGAFT4- CONTRACT REPORT D -77-4 TRANSFORMATIONS OF HEAVY METALS AND PLANT NUTRIENTS IN DREDGED SEDIMENTS AS AFFECTED BY...Engineers, U. S. Army C=) Washington, D . C. 20314 Q-2 Under Contract No. DACW39-74-C-0076 CM-1 (DMRP Work Unit No. IC05) Monitored by Environmental...Sa70mB COMPLETING FOM T -IMPORT PUNIeRf oTACSINN ESCTLGNME Contract Report D -77-4GOTACSINN C NTSAAOGUMR RAAN SFORATIONS OFPEAV71ETALS AND

  10. Impact of selection on maize root traits and rhizosphere interactions

    NASA Astrophysics Data System (ADS)

    Schmidt, J. E.; Gaudin, A. C. M.

    2017-12-01

    Effects of domestication and breeding on maize have been well-characterized aboveground, but impacts on root traits and rhizosphere processes remain unclear. Breeding in high-inorganic-input environments may have negatively affected the ability of modern maize to acquire nutrients through foraging and microbial interactions in marginal and/or organically managed soils. Twelve maize genotypes representing a selection gradient (teosintes, landraces, open-pollinated parents of modern elite germplasm, and modern hybrids released 1934-2015) were grown in three soils varying in intensity of long-term management (unfertilized, organic, conventional) in the greenhouse. Recruitment of rhizosphere microbial communities, nutrient acquisition, and plant productivity were affected by genotype-by-soil interactions. Maize genotypes exhibit significant variation in their ability to obtain nutrients from soils of different management history, indicating the potential for re-integration of beneficial root and rhizosphere traits to increase adaptation to low-input agroecosystems.

  11. Feeding the vegan infant and child.

    PubMed

    Truesdell, D D; Acosta, P B

    1985-07-01

    Nutrients that may be deficient in diets of vegetarian infants and preschoolers and that affect growth and development are energy, protein, calcium, iron, zinc, riboflavin, and vitamins B-12 and D. Reasons for these nutrient deficiencies include: limited volumetric capacity of the stomach of infants, toddlers, and preschoolers; low-caloric-density foods eaten by vegans; limited food choices; and restriction of number of meals and snacks eaten by vegan children. Suggestions are made for meeting the energy and nutrient needs of infants and children within the food ways of their families.

  12. Effects of upland disturbance and instream restoration on hydrodynamics and ammonium uptake in headwater streams

    USGS Publications Warehouse

    Roberts, B.J.; Mulholland, P.J.; Houser, J.N.

    2007-01-01

    Delivery of water, sediments, nutrients, and organic matter to stream ecosystems is strongly influenced by the catchment of the stream and can be altered greatly by upland soil and vegetation disturbance. At the Fort Benning Military Installation (near Columbus, Georgia), spatial variability in intensity of military training results in a wide range of intensities of upland disturbance in stream catchments. A set of 8 streams in catchments spanning this upland disturbance gradient was selected for investigation of the impact of disturbance intensity on hydrodynamics and nutrient uptake. The size of transient storage zones and rates of NH4+ uptake in all study streams were among the lowest reported in the literature. Upland disturbance did not appear to influence stream hydrodynamics strongly, but it caused significant decreases in instream nutrient uptake. In October 2003, coarse woody debris (CWD) was added to 1/2 of the study streams (spanning the disturbance gradient) in an attempt to increase hydrodynamic and structural complexity, with the goals of enhancing biotic habitat and increasing nutrient uptake rates. CWD additions had positive short-term (within 1 mo) effects on hydrodynamic complexity (water velocity decreased and transient storage zone cross-sectional area, relative size of the transient storage zone, fraction of the median travel time attributable to transient storage over a standardized length of 200 m, and the hydraulic retention factor increased) and nutrient uptake (NH4+ uptake rates increased). Our results suggest that water quality in streams with intense upland disturbances can be improved by enhancing instream biotic nutrient uptake capacity through measures such as restoring stream CWD. ?? 2007 by The North American Benthological Society.

  13. Innovations in Food Chemistry and Processing to Enhance the Nutrient Profile of the White Potato in All Forms12

    PubMed Central

    Decker, Eric A.; Ferruzzi, Mario G.

    2013-01-01

    Potatoes can be an important part of a balanced diet because they are an excellent source of many nutrients, including nutrients that are commonly underconsumed (dietary fiber and potassium). Despite the existence of many positive nutrients in potatoes, the popular press has recently aligned potatoes, and particularly fried potatoes, with an unhealthy diet. This article examines the nutritional content of potatoes and how these nutrients are affected by cooking and other food-processing operations. In addition, it examines how the nutritional content of potatoes is altered by cooking methods and how fried potatoes can have wide variations in fat content depending on the cooking method. Finally, the potential of new food-processing technologies to improve the nutritional content of cooked potatoes is evaluated. PMID:23674803

  14. Forest Floor Decomposition Following Hurricane Litter Inputs in Several Puerto Rican Forests

    Treesearch

    Rebecca Ostertag; Frederick N. Scatena; Whendee L. Silver

    2003-01-01

    Hurricanes affect ecosystem processes by altering resource availability and heterogeneity, but the spatial and temporal signatures of these events on biomass and nutrient cycling processes are not well understood. We examined mass and nutrient inputs of hurricane-derived litter in six tropical forests spanning three life zones in northeastern Puerto Rico after the...

  15. Combating Human Micronutrient Deficiencies through Soil Management Practices that Enhance Bioavailability of Nutrients to Plants

    ERIC Educational Resources Information Center

    O'Meara, Mary

    2009-01-01

    Micronutrient malnutrition affects the health and well being of 3 billion people globally. Identifying means to improve the micronutrient density in the edible portions of crops is an important way to combat nutrient deficiencies. By studying how plants obtain micronutrients from the soil, we can develop methods to enhance uptake. Although more…

  16. Tree harvest in an experimental sand ecosystem: plant effects on nutrient dynamics and solute generation.

    Treesearch

    C. K. Keller; R. O' Brien; J. R. Havig; J. L. Smith; B. T. Bormann; D. Wang

    2006-01-01

    The hydrochemical signatures of forested ecosystems are known to be determined by a time-variant combination of physical-hydrologic, geochemical, and biologic processes. We studied subsurface potassium (K), calcium (Ca), and nitrate (NO3) in an experimental red-pine mesocosm to determine how trees affect the behavior of these nutrients in soil...

  17. Empirical modeling of the impact of Mollisol soils variation on performance of Cuphea: A potential oilseed crop

    USDA-ARS?s Scientific Manuscript database

    Production potential of many soils is affected by low supply of nutrients due to adverse constraints or spatio-temporal variation of soil physical and chemical properties. New oilseed crops differ in their nutrient needs for maximum performance in different soils and may not be able to economically ...

  18. Moderate nutrient restriction influences expression of genes impacting production efficiencies of beef cattle in fetal liver, muscle and cerebrum by day 50 of gestation

    USDA-ARS?s Scientific Manuscript database

    We hypothesized that a moderate maternal nutrient restriction during the first 50 days of gestation in beef heifers would affect expression of genes impacting production efficiency phenotypes in the fetal liver, muscle and cerebrum. Fourteen Angus-cross heifers were estrus synchronized and assigned ...

  19. Impacts of elevated atmospheric CO2 on nutrient content and yield of important food crops

    USDA-ARS?s Scientific Manuscript database

    One of the many ways that climate change may affect human health is by altering the nutrient content of food crops. However, previous attempts to study the effects of increased atmospheric CO2 on crop nutrition have been limited by small sample sizes and/or artificial growing conditions. Here we p...

  20. Advances and considerations in technologies for growing, imaging, and analyzing 3-D root system architecture

    USDA-ARS?s Scientific Manuscript database

    The ability of a plant to mine the soil for nutrients and water is determined by how, where, and when roots are arranged in the soil matrix. The capacity of plant to maintain or improve its yield under limiting conditions, such as nutrient deficiency or drought, is affected by root system architectu...

  1. Assembly and intracellular delivery of quantum dot-fluorescent protein bioconjugates

    NASA Astrophysics Data System (ADS)

    Medintz, Igor L.; Pons, Thomas; Delehanty, James B.; Susumu, Kimihiro; Dawson, Philip E.; Mattoussi, Hedi

    2008-02-01

    We have previously assembled semiconductor quantum dot (QD)-based fluorescence resonance energy transfer (FRET) sensors that can specifically detect nutrients, explosives or enzymatic activity. These sensors utilized the inherent benefits of QDs as FRET donors to optimize signal transduction. In this report we functionalize QDs with the multi-subunit multi-chromophore b-phycoerythrin (b-PE) light harvesting complex using biotin-Streptavidin binding. FRET and gel electrophoretic analyses were used to characterize and confirm the QD-b-PE self-assembly. We found that immobilizing additional cell-penetrating peptides on the nanocrystal surface along with the b-PE was the key factor allowing the mixed surface QD-cargos to undergo endocytosis and intracellular delivery. Our findings on the intracellular uptake promoted by CPP were compared to those collected using microinjection technique, where QD-assemblies were delivered directly into the cytoplasm; this strategy allows bypassing of the endocytic uptake pathway. Intracellular delivery of multifunctional QD-fluorescent protein assemblies has potential applications for use in protein tracking, sensing and diagnostics.

  2. Changes in litter quality induced by nutrient addition alter litter decomposition in an alpine meadow on the Qinghai-Tibet Plateau

    PubMed Central

    Zhu, Wenyan; Wang, Jinzhou; Zhang, Zhenhua; Ren, Fei; Chen, Litong; He, Jin-Sheng

    2016-01-01

    The effects of nitrogen (N) and phosphorus (P) addition on litter decomposition are poorly understood in Tibetan alpine meadows. Leaf litter was collected from plots within a factorial N × P addition experiment and allowed to decompose over 708 days in an unfertilized plot to determine the effects of N and/or P addition on litter decomposition. Results showed that nutrient addition significantly affected initial P and P-related biochemical properties of litter from all four species. However, the responses of litter N and N-related biochemical properties to nutrient addition were quite species-specific. Litter C decomposition and N release were species-specific. However, N and P addition significantly affected litter P release. Ratios of Hemicellulose + Cellulose to N and P were significantly related to litter C decomposition; C:N ratio was a determinant of litter N release; and C:P and (Hemicellulose + Cellulose):P controlled litter P release. Overall, litter C decomposition was controlled by litter quality of different plant species, and strongly affected by P addition. Increasing N availability is likely to affect litter C decomposition more indirectly by shifting plant species composition than directly by improving litter quality, and may accelerate N and P cycles, but shift the ecosystem to P limitation. PMID:27694948

  3. Nutritional questions relevant to space flight

    NASA Technical Reports Server (NTRS)

    Lane, Helen W.; Schulz, Leslie O.

    1992-01-01

    This historical review of nutritionally related research in the U.S. and Soviet space programs discusses the uses of nutrition as a countermeasure to the effects of microgravity, with respect to body composition and to exercise. Available information is reviewed from space and ground research in the nutritional requirements for energy, protein, fluids, electrolytes, vitamins, and minerals. Past, present, and future systems for nutrient delivery in space are described, and finally, future directions and challenges are presented.

  4. Clinical Recommendations for the Use of Islet Cell Autoantibodies to Distinguish Autoimmune and Non-Autoimmune Gestational Diabetes.

    PubMed

    Haller-Kikkatalo, Kadri; Uibo, Raivo

    2016-02-01

    Gestational diabetes mellitus (GDM) is defined as carbohydrate intolerance that begins or is first recognized during pregnancy. The prevalence of GDM is highly variable, depending on the population studied, and reflects the underlying pattern of diabetes in the population. GDM manifests by the second half of pregnancy and disappears following delivery in most cases, but is associated with the risk of subsequent diabetes development. Normal pregnancy induces carbohydrate intolerance to favor the availability of nutrients for the fetus, which is compensated by increased insulin secretion from the maternal pancreas. Pregnancy shares similarities with adiposity in metabolism to save energy, and both conditions favor the development of insulin resistance (IR) and low-grade inflammation. A highly complicated network of modified regulatory mechanisms may primarily affect carbohydrate metabolism by promoting autoimmune reactions to pancreatic β cells and affecting insulin function. As a result, diabetes development during pregnancy is facilitated. Depending on a pregnant woman's genetic susceptibility to diabetes, autoimmune mechanisms or IR are fundamental to the development autoimmune or non-autoimmune GDM, respectively. Pregnancy may facilitate the identification of women at risk of developing diabetes later in life; autoimmune and non-autoimmune GDM may be early markers of the risk of future type 1 and type 2 diabetes, respectively. The most convenient and efficient way to discriminate GDM types is to assess pancreatic β-cell autoantibodies along with diagnosing diabetes in pregnancy.

  5. Basal autophagy prevents autoactivation or enhancement of inflammatory signals by targeting monomeric MyD88.

    PubMed

    Into, Takeshi; Horie, Toshi; Inomata, Megumi; Gohda, Jin; Inoue, Jun-Ichiro; Murakami, Yukitaka; Niida, Shumpei

    2017-04-21

    Autophagy, the processes of delivery of intracellular components to lysosomes, regulates induction of inflammation. Inducible macroautophagy degrades inflammasomes and dysfunctional mitochondria to downregulate inflammatory signals. Nonetheless, the effects of constitutive basal autophagy on inflammatory signals are largely unknown. Here, we report a previously unknown effect of basal autophagy. Lysosomal inhibition induced weak inflammatory signals in the absence of a cellular stimulus and in the presence of a nutrient supply, and their induction was impaired by MyD88 deficiency. During lysosomal inhibition, MyD88 was accumulated, and overabundant MyD88 autoactivated downstream signaling or enhanced TLR/IL-1R-mediated signaling. MyD88 is probably degraded via basal microautophagy because macroautophagy inhibitors, ATG5 deficiency, and an activator of chaperone-mediated autophagy did not affect MyD88. Analysis using a chimeric protein whose monomerization/dimerization can be switched revealed that monomeric MyD88 is susceptible to degradation. Immunoprecipitation of monomeric MyD88 revealed its interaction with TRAF6. In TRAF6-deficient cells, degradation of basal MyD88 was enhanced, suggesting that TRAF6 participates in protection from basal autophagy. Thus, basal autophagy lowers monomeric MyD88 expression, and thereby autoactivation of inflammatory signals is prevented. Given that impairment of lysosomes occurs in various settings, our results provide novel insights into the etiology of inflammatory signals that affect consequences of inflammation.

  6. On the Complexity of Nutrient Transport in a Large Watershed in Ohio

    NASA Astrophysics Data System (ADS)

    Schwartz, F. W.; Allen, G.

    2009-12-01

    This paper examines key features of the hydrobiologic setting in controlling the cycling of nutrients through the major streams and rivers of a large agriculturally dominated watershed in central Ohio. The particular focus is on the roles of extreme rainfall events in generating nutrients, and role of reservoirs in attenuating nutrient concentrations. The study also highlights major gaps in process knowledge even in the face in the face of extensive regulatory and other monitoring. Although it has been recognized that reservoirs can significantly affect surface-water flows in watersheds, there is a growing recognition of the need for expanded and complementary studies to understand their role in nutrient transport. The study area is located in central Ohio and includes the entire Upper Scioto and the northern portion of the Lower Scioto River basins, an area encompassing approximately 9984 km2. Five of the sub-watersheds contain major surface-water storage reservoirs. Two watersheds are without reservoirs. There is intensive agriculture within the study area with corn and soybeans as the dominant crops. Tile drainage of fields provides an efficient and rapid connection of agricultural lands to surface waters, facilitating the loading of fertilizers and agrochemicals to surface streams. Storm flows in spring months that coincide with fertilizer applications often provide nitrate concentrations in excess of 10 mg/L as N. In spite of years of routine sampling for regulatory purposes, little is known about nutrient loading patterns during the few, brief, extreme events each year. Interpretations of a high resolution temporal chemical record of sampling on the Scioto River is frustrated by the complexity of loading and mixing as tributaries from sub-watersheds join the main stem of the Scioto River and nutrient utilization within the large reservoirs. Even with literally thousands of individual chemical measurements, extensive stream and precipitation data, the details of processes affecting nutrient transport remain uncertain.

  7. Grafting: A Technique to Modify Ion Accumulation in Horticultural Crops

    PubMed Central

    Nawaz, Muhammad A.; Imtiaz, Muhammad; Kong, Qiusheng; Cheng, Fei; Ahmed, Waqar; Huang, Yuan; Bie, Zhilong

    2016-01-01

    Grafting is a centuries-old technique used in plants to obtain economic benefits. Grafting increases nutrient uptake and utilization efficiency in a number of plant species, including fruits, vegetables, and ornamentals. Selected rootstocks of the same species or close relatives are utilized in grafting. Rootstocks absorb more water and ions than self-rooted plants and transport these water and ions to the aboveground scion. Ion uptake is regulated by a complex communication mechanism between the scion and rootstock. Sugars, hormones, and miRNAs function as long-distance signaling molecules and regulate ion uptake and ion homeostasis by affecting the activity of ion transporters. This review summarizes available information on the effect of rootstock on nutrient uptake and utilization and the mechanisms involved. Information on specific nutrient-efficient rootstocks for different crops of commercial importance is also provided. Several other important approaches, such as interstocking (during double grafting), inarching, use of plant-growth-promoting rhizobacteria, use of arbuscular mycorrhizal fungi, use of plant growth substances (e.g., auxin and melatonin), and use of genetically engineered rootstocks and scions (transgrafting), are highlighted; these approaches can be combined with grafting to enhance nutrient uptake and utilization in commercially important plant species. Whether the rootstock and scion affect each other's soil microbiota and their effect on the nutrient absorption of rootstocks remain largely unknown. Similarly, the physiological and molecular bases of grafting, crease formation, and incompatibility are not fully identified and require investigation. Grafting in horticultural crops can help reveal the basic biology of grafting, the reasons for incompatibility, sensing, and signaling of nutrients, ion uptake and transport, and the mechanism of heavy metal accumulation and restriction in rootstocks. Ion transporter and miRNA-regulated nutrient studies have focused on model and non-grafted plants, and information on grafted plants is limited. Such information will improve the development of nutrient-efficient rootstocks. PMID:27818663

  8. Nutrient cycling, connectivity, and free-floating plant abundance in backwater lakes of the Upper Mississippi River

    USGS Publications Warehouse

    Houser, Jeff N.; Giblin, Shawn M.; James, William F.; Langrehr, H.A.; Rogala, James T.; Sullivan, John F.; Gray, Brian R.

    2013-01-01

    River eutrophication may cause the formation of dense surface mats of free floating plants (FFP; e.g., duckweeds and filamentous algae) which may adversely affect the ecosystem. We investigated associations among hydraulic connectivity to the channel, nutrient cycling, FFP, submersed aquatic vegetation (SAV), and dissolved oxygen concentration (DO) in ten backwater lakes of the Upper Mississippi River (UMR) that varied in connectivity to the channel. Greater connectivity was associated with higher water column nitrate (NO3-N) concentration, higher rates of sediment phosphorus (P) release, and higher rates of NO3-N flux to the sediments. Rates of sediment P and N (as NH4-N) release were similar to those of eutrophic lakes. Water column nutrient concentrations were high, and FFP tissue was nutrient rich suggesting that the eutrophic condition of the UMR often facilitated abundant FFP. However, tissue nutrient concentrations, and the associations between FFP biomass and water column nutrient concentrations, suggested that nutrients constrained FFP abundance at some sites. FFP abundance was positively associated with SAV abundance and negatively associated with dissolved oxygen concentration. These results illustrate important connections among hydraulic connectivity, nutrient cycling, FFP, SAV, and DO in the backwaters of a large, floodplain river.

  9. Logging residue removal after thinning in boreal forests: long-term impact on the nutrient status of Norway spruce and Scots pine needles.

    PubMed

    Luiro, Jukka; Kukkola, Mikko; Saarsalmi, Anna; Tamminen, Pekka; Helmisaari, Heljä-Sisko

    2010-01-01

    The aim of this study was to compare how conventional stem harvesting (CH) and whole-tree harvesting (WTH) in the first, and in some cases also in the second, thinning affect the needle nutrient status of Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies (L.) Karst.) stands in Finland. A series of 12 long-term field experiments was studied. The experiments were established during 1978-86. The effects of logging residue removal after thinnings on the needle nutrient concentrations were generally minor and without any overall trends, but there were differences between experiments. Trees tend to maintain their current needle nutrient concentrations at the same level by re-utilizing the nutrients stored in the older tissues and by changing C allocation in the whole tree. Thus, needle analysis should be combined with stem growth data in order to achieve a more comprehensive understanding of the effects of WTH on the nutrient status of trees.

  10. Species richness alters spatial nutrient heterogeneity effects on above-ground plant biomass.

    PubMed

    Xi, Nianxun; Zhang, Chunhui; Bloor, Juliette M G

    2017-12-01

    Previous studies have suggested that spatial nutrient heterogeneity promotes plant nutrient capture and growth. However, little is known about how spatial nutrient heterogeneity interacts with key community attributes to affect plant community production. We conducted a meta-analysis to investigate how nitrogen heterogeneity effects vary with species richness and plant density. Effect size was calculated using the natural log of the ratio in plant biomass between heterogeneous and homogeneous conditions. Effect sizes were significantly above zero, reflecting positive effects of spatial nutrient heterogeneity on community production. However, species richness decreased the magnitude of heterogeneity effects on above-ground biomass. The magnitude of heterogeneity effects on below-ground biomass did not vary with species richness. Moreover, we detected no modification in heterogeneity effects with plant density. Our results highlight the importance of species richness for ecosystem function. Asynchrony between above- and below-ground responses to spatial nutrient heterogeneity and species richness could have significant implications for biotic interactions and biogeochemical cycling in the long term. © 2017 The Author(s).

  11. The effect of pH on phosphorus availability and speciation in an aquaponics nutrient solution.

    PubMed

    Cerozi, Brunno da Silva; Fitzsimmons, Kevin

    2016-11-01

    The interaction between the main ions in aquaponics nutrient solutions affects chemical composition and availability of nutrients, and nutrient uptake by plant roots. This study determined the effect of pH on phosphorus (P) speciation and availability in an aquaponics nutrient solution and used Visual MINTEQ to simulate P species and P activity. In both experimental and simulated results, P availability decreased with increase in pH of aquaponics nutrient solutions. According to simulations, P binds to several cations leaving less free phosphate ions available in solution. High pH values resulted in the formation of insoluble calcium phosphate species. The study also demonstrated the importance of organic matter and alkalinity in keeping free phosphate ions in solution at high pH ranges. It is recommended though that pH in aquaponics systems is maintained at a 5.5-7.2 range for optimal availability and uptake by plants. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Maternal–Fetal Nutrient Transport in Pregnancy Pathologies: The Role of the Placenta

    PubMed Central

    Brett, Kendra Elizabeth; Ferraro, Zachary Michael; Yockell-Lelievre, Julien; Gruslin, Andrée; Adamo, Kristi Bree

    2014-01-01

    Appropriate in utero growth is essential for offspring development and is a critical contributor to long-term health. Fetal growth is largely dictated by the availability of nutrients in maternal circulation and the ability of these nutrients to be transported into fetal circulation via the placenta. Substrate flux across placental gradients is dependent on the accessibility and activity of nutrient-specific transporters. Changes in the expression and activity of these transporters is implicated in cases of restricted and excessive fetal growth, and may represent a control mechanism by which fetal growth rate attempts to match availability of nutrients in maternal circulation. This review provides an overview of placenta nutrient transport with an emphasis on macro-nutrient transporters. It highlights the changes in expression and activity of these transporters associated with common pregnancy pathologies, including intrauterine growth restriction, macrosomia, diabetes and obesity, as well as the potential impact of maternal diet. Molecular signaling pathways linking maternal nutrient availability and placenta nutrient transport are discussed. How sexual dimorphism affects fetal growth strategies and the placenta’s response to an altered intrauterine environment is considered. Further knowledge in this area may be the first step in the development of targeted interventions to help optimize fetal growth. PMID:25222554

  13. Influence of light presence and biomass concentration on nutrient kinetic removal from urban wastewater by Scenedesmus obliquus.

    PubMed

    Ruiz, J; Arbib, Z; Alvarez-Díaz, P D; Garrido-Pérez, C; Barragán, J; Perales, J A

    2014-05-20

    This work was aimed at studying the effect of light-darkness and high-low biomass concentrations in the feasibility of removing nitrogen and phosphorus from urban treated wastewater by the microalga Scenedesmus obliquus. Laboratory experiments were conducted in batch, where microalgae were cultured under different initial biomass concentrations (150 and 1500mgSSl(-1)) and light conditions (dark or illuminated). Nutrient uptake was more dependent on internal nutrient content of the biomass than on light presence or biomass concentration. When a maximum nitrogen or phosphorus content in the biomass was reached (around 8% and 2%, respectively), the removal of that nutrient was almost stopped. Biomass concentration affected more than light presence on the nutrient removal rate, increasing significantly with its increase. Light was only required to remove nutrients when the maximum nutrient storage capacity of the cells was reached and further growth was therefore needed. Residence times to maintain a stable biomass concentration, avoiding the washout of the reactor, were much higher than those needed to remove the nutrients from the wastewater. This ability to remove nutrients in the absence of light could lead to new configurations of reactors aimed to wastewater treatment. Copyright © 2014 Elsevier B.V. All rights reserved.

  14. Rainfall-induced nutrient losses from manure-fertilized farmland in an alluvial plain.

    PubMed

    Wang, Yiyao; Li, Huaizheng; Xu, Zuxin

    2016-01-01

    Nutrient transport and loss in farmlands are affected by factors such as land cover, fertilization, soil type, rainfall, and management practices. We investigated the temporal and spatial changes in macronutrient transport and loss after fertilization and precipitation in manure-fertilized eggplant farmland in an alluvial plain. Upon adding topical fertilizer, concentrations of most nutrients in runoff and groundwater increased, and nitrogen runoff increased from 22.11 to 35.81 kg/ha, although eggplant yield did not increase correspondingly. Incorporation of fertilizer by plowing reduced nutrient losses (nitrogen runoff/fertilizer decreased from 18.40 to 12.29 %). Measurements taken along the nutrient transport route (runoff, drainage ditch, groundwater, river water, and finally rainfall) revealed that concentrations of most nutrients declined at each stage. Nutrient characteristics varied by transport, and the forms of nitrogen and phosphorus differed greatly between runoff and groundwater (nitrate/nitrogen in runoff was ~43.49 %, while in groundwater ~5.41 %). Most nutrient concentrations in runoff decreased greatly during the planting season (total nitrogen decreased from 62.25 to 4.17 mg/L), correlated positively with temperature and stage of plant growth, but little temporal change was observed in groundwater. This field investigation during one planting season exemplifies the basic principles of nutrient loss and transport from manure-fertilized farmland in an alluvial plain.

  15. Challenges in researching violence affecting health service delivery in complex security environments.

    PubMed

    Foghammar, Ludvig; Jang, Suyoun; Kyzy, Gulzhan Asylbek; Weiss, Nerina; Sullivan, Katherine A; Gibson-Fall, Fawzia; Irwin, Rachel

    2016-08-01

    Complex security environments are characterized by violence (including, but not limited to "armed conflict" in the legal sense), poverty, environmental disasters and poor governance. Violence directly affecting health service delivery in complex security environments includes attacks on individuals (e.g. doctors, nurses, administrators, security guards, ambulance drivers and translators), obstructions (e.g. ambulances being stopped at checkpoints), discrimination (e.g. staff being pressured to treat one patient instead of another), attacks on and misappropriation of health facilities and property (e.g. vandalism, theft and ambulance theft by armed groups), and the criminalization of health workers. This paper examines the challenges associated with researching the context, scope and nature of violence directly affecting health service delivery in these environments. With a focus on data collection, it considers how these challenges affect researchers' ability to analyze the drivers of violence and impact of violence. This paper presents key findings from two research workshops organized in 2014 and 2015 which convened researchers and practitioners in the fields of health and humanitarian aid delivery and policy, and draws upon an analysis of organizational efforts to address violence affecting healthcare delivery and eleven in-depth interviews with representatives of organizations working in complex security environments. Despite the urgency and impact of violence affecting healthcare delivery, there is an overall lack of research that is of health-specific, publically accessible and comparable, as well as a lack of gender-disaggregated data, data on perpetrator motives and an assessment of the 'knock-on' effects of violence. These gaps limit analysis and, by extension, the ability of organizations operating in complex security environments to effectively manage the security of their staff and facilities and to deliver health services. Increased research collaboration among aid organizations, researchers and multilateral organizations, such as the WHO, is needed to address these challenges. Copyright © 2016 Elsevier Ltd. All rights reserved.

  16. Functionalized and graft copolymers of chitosan and its pharmaceutical applications.

    PubMed

    Bhavsar, Chintan; Momin, Munira; Gharat, Sankalp; Omri, Abdelwahab

    2017-10-01

    Chitosan is the second most abundant natural polysaccharide. It belongs a family of polycationic polymers comprised of repetitive units of glucosamine and N-acetylglucosamine. Its biodegradability, nontoxicity, non-immunogenicity and biocompatibility along with properties like mucoadhesion, fungistatic and bacteriogenic have made chitosan an appreciated polymer with numerous applications in the pharmaceutical, comestics and food industry. However, the limited solubility of chitosan at alkaline and neutral pH limits its widespread commercial use. This can be circumvented by fabrication of chitosan by graft copolymerization with acyl, alkyl, monomeric and polymeric moieties. Areas covered: Modifications like quarterization, thiolation, acylation and grafting result in copolymers with higher mucoadhesion strength, increased hydrophobic interactions (advantageous in hydrophobic drug entrapment), and increased solubility in alkaline pH, the ability for adsorption of metal ions, protein and peptide delivery and nutrient delivery. Insights on methods of polymerization, including atomic transfer radical polymerization and click chemistry are discussed. Applications of such modified chitosan copolymers in medical and surgical, and drug delivery, including nasal, oral and buccal delivery have also been covered. Expert opinion: Despite a number of successful investigations, commercialization of chitosan copolymers still remains a challenge. Further advancements in polymerization techniques may address the unmet needs of the healthcare industry.

  17. The Biogeochemical Role of Baleen Whales and Krill in Southern Ocean Nutrient Cycling

    PubMed Central

    Ratnarajah, Lavenia; Bowie, Andrew R.; Lannuzel, Delphine; Meiners, Klaus M.; Nicol, Stephen

    2014-01-01

    The availability of micronutrients is a key factor that affects primary productivity in High Nutrient Low Chlorophyll (HNLC) regions of the Southern Ocean. Nutrient supply is governed by a range of physical, chemical and biological processes, and there are significant feedbacks within the ecosystem. It has been suggested that baleen whales form a crucial part of biogeochemical cycling processes through the consumption of nutrient-rich krill and subsequent defecation, but data on their contribution are scarce. We analysed the concentration of iron, cadmium, manganese, cobalt, copper, zinc, phosphorus and carbon in baleen whale faeces and muscle, and krill tissue using inductively coupled plasma mass spectrometry. Metal concentrations in krill tissue were between 20 thousand and 4.8 million times higher than typical Southern Ocean HNLC seawater concentrations, while whale faecal matter was between 276 thousand and 10 million times higher. These findings suggest that krill act as a mechanism for concentrating and retaining elements in the surface layer, which are subsequently released back into the ocean, once eaten by whales, through defecation. Trace metal to carbon ratios were also higher in whale faeces compared to whale muscle indicating that whales are concentrating carbon and actively defecating trace elements. Consequently, recovery of the great whales may facilitate the recycling of nutrients via defecation, which may affect productivity in HNLC areas. PMID:25469984

  18. The biogeochemical role of baleen whales and krill in Southern Ocean nutrient cycling.

    PubMed

    Ratnarajah, Lavenia; Bowie, Andrew R; Lannuzel, Delphine; Meiners, Klaus M; Nicol, Stephen

    2014-01-01

    The availability of micronutrients is a key factor that affects primary productivity in High Nutrient Low Chlorophyll (HNLC) regions of the Southern Ocean. Nutrient supply is governed by a range of physical, chemical and biological processes, and there are significant feedbacks within the ecosystem. It has been suggested that baleen whales form a crucial part of biogeochemical cycling processes through the consumption of nutrient-rich krill and subsequent defecation, but data on their contribution are scarce. We analysed the concentration of iron, cadmium, manganese, cobalt, copper, zinc, phosphorus and carbon in baleen whale faeces and muscle, and krill tissue using inductively coupled plasma mass spectrometry. Metal concentrations in krill tissue were between 20 thousand and 4.8 million times higher than typical Southern Ocean HNLC seawater concentrations, while whale faecal matter was between 276 thousand and 10 million times higher. These findings suggest that krill act as a mechanism for concentrating and retaining elements in the surface layer, which are subsequently released back into the ocean, once eaten by whales, through defecation. Trace metal to carbon ratios were also higher in whale faeces compared to whale muscle indicating that whales are concentrating carbon and actively defecating trace elements. Consequently, recovery of the great whales may facilitate the recycling of nutrients via defecation, which may affect productivity in HNLC areas.

  19. Role of nutrients and illuminance in predicting the fate of fungal mediated petroleum hydrocarbon degradation and biomass production.

    PubMed

    Ali Khan, Aqib Hassan; Tanveer, Sundus; Anees, Mariam; Muhammad, Yousaf Shad; Iqbal, Mazhar; Yousaf, Sohail

    2016-07-01

    Biodegradation and biomass production are affected by numerous environmental factors including pH, oxygen availability and presence of pollutants. The present study, for the first time, elucidated the effects of nutrients and light on mycodegradation of petroleum hydrocarbons in diesel oil. Seven fungal strains (Aspergillus terreus FA3, Aspergillus niger FA5, Aspergillus terreus FA6, Penicillium chrysogenum FP4, Aspergillus terreus FP6, Aspergillus flavus FP10, and Candida sp. FG1) were used for hydrocarbon degradation under static conditions, in four combinations of nutrient media and illuminance for 45 days. Highest degradation was achieved by Aspergillus terreus FA6 and Candida sp. FG1 under both conditions of light and dark, with nutrient deprived HAF (Hydrocarbon adopted fungi) broth. Under HAF/Dark diesel oil degradation by FA6 and FG1 was 87.3% and 84.3% respectively, while under HAF/Light both FA6 and FG1 performed 84.3% biodegradation. The highest biomass was produced by Aspergillus flavus FP10 in PDB (Potato dextrose broth)/Dark (109.3 mg). Fungal degradation of petroleum hydrocarbons was negatively affected by the presence of other simpler-to-degrade carbon sources in the medium. The biomass production was enhanced by improved nutrient availability and diminished by illuminance. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Effects of nutrient enrichment on the release of dissolved organic carbon and nitrogen by the scleractinian coral Montipora digitata

    NASA Astrophysics Data System (ADS)

    Tanaka, Y.; Ogawa, H.; Miyajima, T.

    2010-09-01

    The effects of nutrient enrichment on the release of dissolved organic carbon and nitrogen (DOC and DON, respectively) from the coral Montipora digitata were investigated in the laboratory. Nitrate (NO3 -) and phosphate (PO4 3-) were supplied to the aquarium to get the final concentrations of 10 and 0.5 μmol l-1, respectively, and the corals were incubated for 8 days. The release rate of DON per unit coral surface area significantly decreased after the nutrient enrichment, while the release rate of DOC was constant. Because the chlorophyll a (chl a) content of zooxanthellae per unit surface area increased, the release rate of DOC significantly decreased when normalized to unit chl a. These results suggested that the incorporation of NO3 - and PO4 3- stimulated the synthesis of new cellular components in the coral colonies and consequently, reduced extracellular release of DOC and DON. Actually, significant increase in N and P contents relative to C content was observed in the coral’s tissue after the nutrient enrichment. The present study has concluded that inorganic nutrient enrichment not only affects coral-algal metabolism inside the colony but also affects a microbial community around the coral because the organic matter released from corals functions as energy carrier in the coral reef ecosystem.

  1. Bioaccessibility of nutrients and micronutrients from dispersed food systems: impact of the multiscale bulk and interfacial structures.

    PubMed

    Marze, Sébastien

    2013-01-01

    Many food systems are dispersed systems, that is, they possess at least two immiscible phases. This is generally due to the coexistence of domains with different physicochemical properties separated by many interfaces which control the apparent thermodynamic equilibrium. This feature was and is still largely studied to design pharmaceutical delivery systems. In food science, the recent intensification of in vitro digestion tests to complement the in vivo ones holds promises in the identification of the key parameters controlling the bioaccessibility of nutrients and micronutrients. In this review, we present the developments of in vitro digestion tests for dispersed food systems (mainly emulsions, dispersions and gels). We especially highlight the evidences detailing the roles of the constituting multiscale structures. In a perspective section, we show the potential of structured interfaces to allow controlled bioaccessibility.

  2. CELSS Program Meeting

    NASA Technical Reports Server (NTRS)

    Tremor, John W.; Macelroy, Robert D.

    1987-01-01

    A meeting on the potential contributions of plant science to the goals of Controlled Ecological Life Support System (CELSS) research produced discussions that helped to focus on a variety of topics. In the area of volatiles and soluble organics, microbial activity, disease, and productivity, participants emphasized the need to know more about the consequences of closure for the growth of plants. Under nutrient delivery systems, the problems focus on the need to maintain a stable, optimum nutrient system. Lighting systems discussions emphasized unique methods of direct lighting and development of improved irradiation sources. Flight experiment opportunities were outlined by one speaker. Documentation of the Plant Growth Module was discussed. The last day's discussion focused on the organization of the research group to be involved in the development and use of a two to three cubic meter sealed chamber and ancillary equipment.

  3. Growth of non-Saccharomyces yeasts affects nutrient availability for Saccharomyces cerevisiae during wine fermentation.

    PubMed

    Medina, Karina; Boido, Eduardo; Dellacassa, Eduardo; Carrau, Francisco

    2012-07-02

    Yeast produces numerous secondary metabolites during fermentation that impact final wine quality. Although it is widely recognized that growth of diverse non-Saccharomyces (NS) yeast can positively affect flavor complexity during Saccharomyces cerevisiae wine fermentation, the inability to control spontaneous or co-fermentation processes by NS yeast has restricted their use in winemaking. We selected two NS yeasts from our Uruguayan native collection to study NS-S. cerevisiae interactions during wine fermentation. The selected strains of Hanseniaspora vineae and Metschnikowia pulcherrima had different yeast assimilable nitrogen consumption profiles and had different effects on S. cerevisiae fermentation and growth kinetics. Studies in which we varied inoculum size and using either simultaneous or sequential inoculation of NS yeast and S. cerevisiae suggested that competition for nutrients had a significant effect on fermentation kinetics. Sluggish fermentations were more pronounced when S. cerevisiae was inoculated 24h after the initial stage of fermentation with a NS strain compared to co-inoculation. Monitoring strain populations using differential WL nutrient agar medium and fermentation kinetics of mixed cultures allowed for a better understanding of strain interactions and nutrient addition effects. Limitation of nutrient availability for S. cerevisiae was shown to result in stuck fermentations as well as to reduce sensory desirability of the resulting wine. Addition of diammonium phosphate (DAP) and a vitamin mix to a defined medium allowed for a comparison of nutrient competition between strains. Addition of DAP and the vitamin mix was most effective in preventing stuck fermentations. Copyright © 2012 Elsevier B.V. All rights reserved.

  4. Effects of ultraviolet radiation and nutrients on the structure-function of phytoplankton in a high mountain lake.

    PubMed

    Korbee, Nathalie; Carrillo, Presentación; Mata, M Teresa; Rosillo, Silvia; Medina-Sánchez, Juan Manuel; Figueroa, Félix L

    2012-06-01

    The combined effect of high solar ultraviolet radiation (UVR) and nutrient supply in a phytoplankton community of a high mountain lake is analyzed in a in situ experiment for 6 days with 2 × 2 factorial design. Interactive UVR × nutrient effects on structural and functional variables (algal biomass, chlorophyll a (chl a), primary production (PP), maximal electron transport rate (ETR(max)), and alkaline phosphatase activity (APA)), as well as stoichiometric ones (sestonic N per cell and N:P ratio) were found. Under non-nutrient enriched conditions, no deleterious effects of UVR on structural variables, PP, photosynthetic efficiency and ETR(max) were observed, whereas only particulate and total APA were affected by UVR. However, percentage excreted organic carbon (%EOC), dissolved APA and sestonic C and P per cell increased under UVR, leading to a decrease in algal C:P and N:P ratios. After nutrient enrichment, chl a, total algal biomass and PP were negatively affected by UVR whereas %EOC, ETR(max) and internal C, P and N content increased. We suggest that the mechanism of algal acclimation to UVR in this high UVR flux ecosystem seems to be related to the increase of internal algal P-content mediated by physiological mechanisms to save P and by a stimulatory UVR effect on dissolved extracellular APA. The mechanism involved in the unmasking effect of UVR after nutrient-enrichment may be the result of a greater sensitivity to UVR-induced cell damage, making the negative UVR effects more evident.

  5. Impact of glyphosate-resistant corn, glyphosate applications and tillage on soil nutrient ratios, exoenzyme activities and nutrient acquisition ratios.

    PubMed

    Jenkins, Michael B; Locke, Martin A; Reddy, Krishna N; McChesney, Daniel S; Steinriede, R Wade

    2017-01-01

    We report results of the last two years of a 7 year field experiment designed to test the null hypothesis: applications of glyphosate on glyphosate-resistant (GR) and non-resistant (non-GR) corn (Zea mays L.) under conventional tillage and no-till would have no effect on soil exoenzymes and microbial activity. Bulk soil (BS) and rhizosphere soil (RS) macronutrient ratios were not affected by either GR or non-GR corn, or glyphosate applications. Differences observed between exoenzyme activities were associated with tillage rather than glyphosate applications. In 2013, nutrient acquisition ratios for bulk and rhizosphere soils indicated P limitations, but sufficient assimilable N. In 2014, P limitations were observed for bulk and rhizosphere soils, in contrast to balanced C and N acquisition ratios in rhizosphere soils. Stoichiometric relationships indicated few differences between glyphosate and non-glyphosate treatments. Negative correlations between C:P and N:P nutrient ratios and nutrient acquisition ratios underscored the inverse relation between soil nutrient status and microbial community exoenzyme activities. Inconsistent relationships between microbial community metabolic activity and exoenzyme activity indicated an ephemeral effect of glyphosate on BS exoenzyme activity. Except for ephemeral effects, glyphosate applications appeared not to affect the function of the BS and RS exoenzymes under conventional tillage or no-till. Published 2016. This article is a U.S. Government work and is in the public domain in the USA. Published 2016. This article is a U.S. Government work and is in the public domain in the USA.

  6. Automated pH Control of Nutrient Solution in a Hydroponic Plant Growth System

    NASA Technical Reports Server (NTRS)

    Smith, B.; Dogan, N.; Aglan, H.; Mortley, D.; Loretan, P.

    1998-01-01

    Over, the years, NASA has played an important role in providing to and the development of automated nutrient delivery and monitoring, systems for growing crops hydroponically for long term space missions. One example are the systems used in the Biomass Production Chamber (BPC) at Kennedy Space Center (KSC). The current KSC monitoring system is based on an engineering workstation using standard analog/digital input/output hardware and custom written software. The monitoring system uses completely separate sensors to provide a check of control sensor accuracy and has the ability to graphically display and store data form past experiment so that they are available for data analysis [Fortson, 1992]. In many cases, growing systems have not been fitted with the kind of automated control systems as used at KSC. The Center for Food and Environmental Systems for Human Exploration of Space (CFESH) located on the campus of Tuskegee University, has effectively grown sweetpotatoes and peanuts hydroponically for the past five years. However they have adjusted the pH electrical conductivity and volume of the hydroponic nutrient solution only manually at times when the solution was to be replenished or changed out according to its protocol (e.g. one-week, two-week, or two-day cycle). But the pH of the nutrient solution flowing through the channel is neither known nor controlled between the update, change out, or replenishment period. Thus, the pH of the nutrient solution is not held at an optimum level over the span of the plant's growth cycle. To solve this dilemma, an automated system for the control and data logging of pH data relative to sweetpotato production using the nutrient film technique (NFT) has been developed, This paper discusses a microprocessor-based system, which was designed to monitor, control, and record the pH of a nutrient solution used for growing sweetpotatoes using NFT.

  7. Nutrient cycles in agricultural systems at sub-catchment scale within the UK and China

    NASA Astrophysics Data System (ADS)

    Bellarby, Jessica; Surridge, Ben; Haygarth, Philip M.; Lai, Xin; Zhang, Guilong; Song, Xiaolong; Zhou, Jianbin; Meng, Fanqiao; Shen, Jianbo; Rahn, Clive; Smith, Laurence; Burke, Sean

    2015-04-01

    Diffuse water pollution from agriculture (DWPA) represents a significant challenge in both the UK and China. The UK has developed policies and practices which seek to mitigate DWPA, yet the risks and adverse impacts of DWPA remain widespread. In contrast, China's past priorities have largely focussed on food security, with an emphasis on increasing food production through high fertiliser application rates with little attention being paid to enhanced nutrient export from land to water and to air. This has contributed to severe environmental problems which are only now beginning to be recognised and addressed. We have prepared nutrient balances (phosphorus and nitrogen) in contrasting agricultural production systems at sub-catchment scale within China and the UK. These draw from a variety of sources ranging from general yearly statistics collected by the respective government to farm surveys. Our aim is to use the resulting nutrient balances to underpin the sharing of knowledge and innovation to mitigate DWPA in both nations. In the UK, the case studies focus on the three Demonstration Test Catchment locations, covering a range of livestock and arable production systems across England. Here, the high frequency monitoring of phosphorus river loads enables the cross-validation of the simple nutrient budget approaches applied in this study. In China, our case studies span kiwi orchard, fruit and vegetable solar greenhouse systems, double cropped rice-wheat and wheat-maize production systems. Substantial differences in nutrient stocks and flows exist between individual production systems both across and within the two countries. These differences will be expressed along the source-mobilisation-delivery-impact continuum that underpins our budgets for both phosphorus and nitrogen. We will present the phosphorus cycles of some case studies and highlight their challenges and relevance at sub-catchment scale. Based on our nutrient budgets, general recommendations can be formulated to mitigate DWPA from farm to policy levels.

  8. Reoxidation of estuarine sediments during simulated resuspension events: Effects on nutrient and trace metal mobilisation

    NASA Astrophysics Data System (ADS)

    Vidal-Durà, Andrea; Burke, Ian T.; Stewart, Douglas I.; Mortimer, Robert J. G.

    2018-07-01

    Estuarine environments are considered to be nutrient buffer systems as they regulate the delivery of nutrients from rivers to the ocean. In the Humber Estuary (UK) seawater and freshwater mixing during tidal cycles leads to the mobilisation of oxic surface sediments (0-1 cm). However, less frequent seasonal events can also mobilise anoxic subsurface (5-10 cm) sediments, which may have further implications for the estuarine geochemistry. A series of batch experiments were carried out on surface and subsurface sediments taken from along the salinity gradient of the Humber Estuary. The aim was to investigate the geochemical processes driving major element (N, Fe, S, and Mn) redox cycling and trace metal behaviour during simulated resuspension events. The magnitude of major nutrient and metal release was significantly greater during the resuspension of outer estuarine sediments rather than from inner estuarine sediments. When comparing resuspension of surface versus subsurface sediment, only the outer estuary experiments showed significant differences in major nutrient behaviour with sediment depth. In general, any ammonium, manganese and trace metals (Cu and Zn) released during the resuspension experiments were rapidly removed from solution as new sorption sites (i.e. Fe/Mn oxyhydroxides) formed. Therefore Humber estuary sediments showed a scavenging capacity for these dissolved species and hence may act as an ultimate sink for these elements. Due to the larger aerial extent of the outer estuary intertidal mudflats in comparison with the inner estuary area, the mobilisation of the outer estuary sediments (more reducing and richer in sulphides and iron) may have a greater impact on the transport and cycling of nutrients and trace metals. Climate change-associated sea level rise combined with an increasing frequency of major storm events in temperate zones, which are more likely to mobilise deeper sediment regions, will impact the nutrient and metal inputs to the coastal waters, and therefore enhance the likelihood of eutrophication in this environment.

  9. Interactive effects of nutrient additions and predation on infaunal communities

    USGS Publications Warehouse

    Posey, M.H.; Alphin, T.D.; Cahoon, L.; Lindquist, D.; Becker, M.E.

    1999-01-01

    Nutrient additions represent an important anthropogenic stress on coastal ecosystems. At moderate levels, increased nutrients may lead to increased primary production and, possibly, to increased biomass of consumers although complex trophic interactions may modify or mask these effects. We examined the influence of nutrient additions and interactive effects of trophic interactions (predation) on benthic infaunal composition and abundances through small-scale field experiments in 2 estuaries that differed in ambient nutrient conditions. A blocked experimental design was used that allowed an assessment of direct nutrient effects in the presence and absence of predation by epibenthic predators as well as an assessment of the independent effects of predation. Benthic microalgal production increased with experimental nutrient additions and was greater when infaunal abundances were lower, but there were no significant interactions between these factors. Increased abundances of one infaunal taxa, Laeonereis culveri, as well as the grazer feeding guild were observed with nutrient additions and a number of taxa exhibited higher abundances with predator exclusion. In contrast to results from freshwater systems there were no significant interactive effects between nutrient additions and predator exclusion as was predicted. The infaunal responses observed here emphasize the importance of both bottom-up (nutrient addition and primary producer driven) and top-down (predation) controls in structuring benthic communities. These processes may work at different spatial and temporal scales, and affect different taxa, making observation of potential interactive effects difficult.

  10. Proposal for a method to estimate nutrient shock effects in bacteria

    PubMed Central

    2012-01-01

    Background Plating methods are still the golden standard in microbiology; however, some studies have shown that these techniques can underestimate the microbial concentrations and diversity. A nutrient shock is one of the mechanisms proposed to explain this phenomenon. In this study, a tentative method to assess nutrient shock effects was tested. Findings To estimate the extent of nutrient shock effects, two strains isolated from tap water (Sphingomonas capsulata and Methylobacterium sp.) and two culture collection strains (E. coli CECT 434 and Pseudomonas fluorescens ATCC 13525) were exposed both to low and high nutrient conditions for different times and then placed in low nutrient medium (R2A) and rich nutrient medium (TSA). The average improvement (A.I.) of recovery between R2A and TSA for the different times was calculated to more simply assess the difference obtained in culturability between each medium. As expected, A.I. was higher when cells were plated after the exposition to water than when they were recovered from high-nutrient medium showing the existence of a nutrient shock for the diverse bacteria used. S. capsulata was the species most affected by this phenomenon. Conclusions This work provides a method to consistently determine the extent of nutrient shock effects on different microorganisms and hence quantify the ability of each species to deal with sudden increases in substrate concentration. PMID:22873690

  11. Phytoplankton Diversity Effects on Community Biomass and Stability along Nutrient Gradients in a Eutrophic Lake.

    PubMed

    Tian, Wang; Zhang, Huayong; Zhao, Lei; Zhang, Feifan; Huang, Hai

    2017-01-20

    The relationship between biodiversity and ecosystem functioning is a central issue in ecology, but how this relationship is affected by nutrient stress is still unknown. In this study, we analyzed the phytoplankton diversity effects on community biomass and stability along nutrient gradients in an artificial eutrophic lake. Four nutrient gradients, varying from slightly eutrophic to highly eutrophic states, were designed by adjusting the amount of polluted water that flowed into the lake. Mean phytoplankton biomass, species richness, and Shannon diversity index all showed significant differences among the four nutrient gradients. Phytoplankton community biomass was correlated with diversity (both species richness and Shannon diversity index), varying from positive to negative along the nutrient gradients. The influence of phytoplankton species richness on resource use efficiency (RUE) also changed from positive to negative along the nutrient gradients. However, the influence of phytoplankton Shannon diversity on RUE was not significant. Both phytoplankton species richness and Shannon diversity had a negative influence on community turnover (measured as community dissimilarity), i.e., a positive diversity-stability relationship. Furthermore, phytoplankton spatial stability decreased along the nutrient gradients in the lake. With increasing nutrient concentrations, the variability (standard deviation) of phytoplankton community biomass increased more rapidly than the average total biomass. Results in this study will be helpful in understanding the phytoplankton diversity effects on ecosystem functioning and how these effects are influenced by nutrient conditions in aquatic ecosystems.

  12. Microgravity does not alter plant stand gas exchange of wheat at moderate light levels and saturating CO2 concentration.

    PubMed

    Monje, O; Stutte, G; Chapman, D

    2005-10-01

    Plant stand gas exchange was measured nondestructively in microgravity during the Photosynthesis Experiment Subsystem Testing and Operations experiment conducted onboard the International Space Station. Rates of evapotranspiration and photosynthesis measured in space were compared with ground controls to determine if microgravity directly affects whole-stand gas exchange of Triticum aestivum. During six 21-day experiment cycles, evapotranspiration was determined continuously from water addition rates to the nutrient delivery system, and photosynthesis was determined from the amount of CO2 added to maintain the chamber CO2 concentration setpoint. Plant stand evapotranspiration, net photosynthesis, and water use efficiency were not altered by microgravity. Although leaf area was significantly reduced in microgravity-grown plants compared to ground control plants, leaf area distribution was not affected enough to cause significant differences in the amounts of light absorbed by the flight and ground control plant stands. Microgravity also did not affect the response of evapotranspiration to changes in chamber vapor pressure difference of 12-day-old wheat plant stands. These results suggest that gravity naïve plants grown at moderate light levels (300 micromol m(-2) s(-1)) behave the same as ground control plants. This implies that future plant-based regenerative life support systems can be sized using 1 g data because water purification and food production rates operate at nearly the same rates as in 1 g at moderate light levels. However, it remains to be verified whether the present results are reproducible in plants grown under stronger light levels.

  13. How does fire affect longleaf pine roots carbohydrates, foliar nutrients, and sapling growth?

    Treesearch

    Eric A. Kuehler; Marry Anne Sword Sayer; C. Dan Andries

    2006-01-01

    In central Louisiana, we conducted a prescribed-fire study in a 5-year-old longleaf pine (Pinus palustris P. Mill.) stand to evaluate the effects of fire on fine-root (2- to 5-mm diameter) carbohydrates, dormant season foliar nutrients, and sapling growth. Control, burn, and nonburned vegetation control treatments were studied using a randomized...

  14. A nutrient-dense, high fiber, fruit-based supplement bar increases HDL, particularly large HDL, lowers homocysteine, and raises glutathione in a 2-week trial

    USDA-ARS?s Scientific Manuscript database

    Dietary intake modulates disease risk, but little is known as to how components within food mixtures affect pathophysiology. Here, a low-calorie, high-fiber, fruit-based nutrient-dense bar of defined composition (e.g., vitamins/minerals, fruit polyphenolics, B-glucan, docosahexaenoic acid (DHA)) app...

  15. Influence of calcium foliar fertilization on plant growth, nutrient concentrations, and fruit quality of papaya.

    USDA-ARS?s Scientific Manuscript database

    Calcium (Ca) is a major plant nutrient that affects cell wall and plasma membrane formation and plays a key role in plant growth and biomass production. It can be used to decrease fruit decay and increase firmness and shelf life. So far, little attention has been paid to investigate the effects of f...

  16. Regulating nutrient allocation in plants

    DOEpatents

    Udvardi, Michael; Yang, Jiading; Worley, Eric

    2014-12-09

    The invention provides coding and promoter sequences for a VS-1 and AP-2 gene, which affects the developmental process of senescence in plants. Vectors, transgenic plants, seeds, and host cells comprising heterologous VS-1 and AP-2 genes are also provided. Additionally provided are methods of altering nutrient allocation and composition in a plant using the VS-1 and AP-2 genes.

  17. The growth and anatomical features of nutrient-deficient seedlings

    Treesearch

    Fred M. Lamb; Wayne K. Murphey

    1968-01-01

    As the tree improvement and genetic programs supply better planting stock, a more suitable environment must be provided if their full potential is to be realized. This will require much more information than we now have on how nutrient deficiencies affect the growth and anatomy of forest trees. The importance of anatomical studies has been shown by Church (1949) and...

  18. Substrate and nutrient limitation of ammonia-oxidizing bacteria and archaea in temperate forest soil

    Treesearch

    J.S. Norman; J.E. Barrett

    2014-01-01

    Ammonia-oxidizing microbes control the rate-limiting step of nitrification, a critical ecosystem process, which affects retention and mobility of nitrogen in soil ecosystems. This study investigated substrate (NH4þ) and nutrient (K and P) limitation of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) in temperate forest soils at Coweeta Hydrologic...

  19. Influences of N-fixing and non-N-fixing vegetation and invasive fish on water chemistry of Hawaiian anchialine ponds

    Treesearch

    B. D. Dudley; Richard MacKenzie; T. S. Sakihara; H. Dulaiova; C. A. Waters; Flint Hughes; R. Ostertag

    2014-01-01

    In coastal waters, it remains unclear how terrestrial invasive species might alter nutrient availability and thus affect bottom-up control of primary production. Anchialine ponds are tidal- and groundwater-fed coastal water bodies without surface connections that provide convenient model systems in which to examine terrestrial to aquatic nutrient flow. To investigate...

  20. Feeding fat from distillers dried grains with solubles to dairy heifers: I. Effects on growth performance and total tract digestibility of nutrients

    USDA-ARS?s Scientific Manuscript database

    The objective of this study was to determine if increased dietary fat from dried distillers grains with solubles (DDGS) in diets of growing heifers affected dry matter intake (DMI), average daily gain (ADG), growth performance, and nutrient digestibility. Thirty-three Holstein heifers (133 ± 18 d ol...

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