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Sample records for ag-cares lamesa tx

  1. Greening America's Capitals - Austin, TX

    EPA Pesticide Factsheets

    Report on the technical assistance project to help Austin, TX, develop a vision for the South Central Waterfront that incorporates green infrastructure to manage stormwater runoff, makes streets safer, and spurs investment.

  2. 33 CFR 80.840 - Sabine Pass, TX to Galveston, TX.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Sabine Pass, TX to Galveston, TX... INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Eighth District § 80.840 Sabine Pass, TX to Galveston, TX. (a) A line drawn from the Sabine Pass East Jetty Light to the seaward end of the Sabine Pass...

  3. 77 FR 19521 - Prevailing Rate Systems; Redefinition of the Austin, TX and Waco, TX, Appropriated Fund Federal...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-02

    ... 3206-AM50 Prevailing Rate Systems; Redefinition of the Austin, TX and Waco, TX, Appropriated Fund... of the Austin, TX, and Waco, TX, appropriated fund Federal Wage System (FWS) wage areas. The final rule redefines Burleson and Lampasas Counties, TX, from the Austin wage area to the Waco wage...

  4. 76 FR 70365 - Prevailing Rate Systems; Redefinition of the Austin, TX and Waco, TX, Appropriated Fund Federal...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-14

    ... Area Brazos, Burleson, and Robertson Counties, TX, comprise the College Station-Bryan, TX MSA. The College Station-Bryan MSA is split between the Austin, TX, and Waco, TX, wage areas. Burleson County is... Brazos County, the core county in the College Station-Bryan MSA, we recommend that the entire...

  5. TX-100 manufacturing final project report.

    SciTech Connect

    Ashwill, Thomas D.; Berry, Derek S.

    2007-11-01

    This report details the work completed under the TX-100 blade manufacturing portion of the Carbon-Hybrid Blade Developments: Standard and Twist-Coupled Prototype project. The TX-100 blade is a 9 meter prototype blade designed with bend-twist coupling to augment the mitigation of peak loads during normal turbine operation. This structural coupling was achieved by locating off axis carbon fiber in the outboard portion of the blade skins. The report will present the tooling selection, blade production, blade instrumentation, blade shipping and adapter plate design and fabrication. The baseline blade used for this project was the ERS-100 (Revision D) wind turbine blade. The molds used for the production of the TX-100 were originally built for the production of the CX-100 blade. The same high pressure and low pressure skin molds were used to manufacture the TX-100 skins. In order to compensate for the difference in skin thickness between the CX-100 and the TX-100, however, a new TX-100 shear web plug and mold were required. Both the blade assembly fixture and the root stud insertion fixture used for the CX-100 blades could be utilized for the TX-100 blades. A production run of seven TX-100 prototype blades was undertaken at TPI Composites during the month of October, 2004. Of those seven blades, four were instrumented with strain gauges before final assembly. After production at the TPI Composites facility in Rhode Island, the blades were shipped to various test sites: two blades to the National Wind Technology Center at the National Renewable Energy Laboratory in Boulder, Colorado, two blades to Sandia National Laboratory in Albuquerque, New Mexico and three blades to the United States Department of Agriculture turbine field test facility in Bushland, Texas. An adapter plate was designed to allow the TX-100 blades to be installed on existing Micon 65/13M turbines at the USDA site. The conclusion of this program is the kick-off of the TX-100 blade testing at the three

  6. 76 FR 73504 - Amendment of Class E Airspace; Alice, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-29

    ... Airport, Agua Dulce, TX, has made this action necessary to enhance the safety and management of Instrument... Old Hoppe Place Airport, Agua Dulce, TX, due to the cancellation of all standard instrument...

  7. 76 FR 40765 - Texas Disaster #TX-00378

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-11

    ... ADMINISTRATION Texas Disaster TX-00378 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of Texas dated 07/05/2011. Incident: Dyer Mills Fire. Incident Period: 06/19/2011 through 06/26/2011. Effective Date:...

  8. 78 FR 48764 - Texas Disaster # TX-00413

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-09

    ... From the Federal Register Online via the Government Publishing Office ] SMALL BUSINESS ADMINISTRATION Texas Disaster TX-00413 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This... applications to: U.S. Small Business Administration, Processing and Disbursement Center, 14925 Kingsport...

  9. 78 FR 36631 - Texas Disaster #TX-00408

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-18

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Texas Disaster TX-00408 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This... Deadline Date: 03/12/2014. ADDRESSES: Submit completed loan applications to: U.S. Small...

  10. 78 FR 72140 - Texas Disaster # TX-00417

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-02

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Texas Disaster TX-00417 AGENCY: U.S. Small Business Administration ACTION: Notice SUMMARY: This is... adversely affected by the disaster: Primary Counties: Caldwell, Hays, Travis. Contiguous Counties:...

  11. 75 FR 13669 - Amendment of Class E Airspace; Dumas, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-23

    ... Instrument Approach Procedures (SIAPs) at Moore County Airport, Dumas, TX, and updates the airport's... rulemaking to amend Class E airspace for Dumas, TX, reconfiguring controlled airspace at Moore County Airport... surface to accommodate SIAPs at Moore County Airport, Dumas, TX. This action also updates the...

  12. 75 FR 31677 - Amendment of Class E Airspace; Austin, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-04

    ... TRANSPORTATION Federal Aviation Administration 14 CFR Part 71 Amendment of Class E Airspace; Austin, TX AGENCY... airspace for the Austin, TX area. Additional controlled airspace is necessary to accommodate new Standard Instrument Approach Procedures (SIAPs) at Austin Executive Airport, Austin, TX. The FAA is taking this...

  13. 77 FR 29874 - Establishment of Class E Airspace; Freer, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-21

    ... Federal Aviation Administration 14 CFR Part 71 Establishment of Class E Airspace; Freer, TX AGENCY... airspace at Freer, TX. Controlled airspace is necessary to accommodate new Area Navigation (RNAV) Standard... Federal Register a notice of proposed rulemaking (NPRM) to amend Class E airspace for the Freer, TX,...

  14. 76 FR 52563 - Special Local Regulations; Sabine River, Orange, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-23

    ... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulations; Sabine River, Orange, TX...) entitled Special Local Regulations; Sabine River, Orange, TX in the Federal Register (76 FR 103). We... Events; Sabine River, Orange, TX. (a) Definitions. As used in this section ``Participant Vessel''...

  15. Big Bend National Park, TX, USA, Mexico

    NASA Technical Reports Server (NTRS)

    1991-01-01

    The Sierra del Carmen of Mexico, across the Rio Grande River from Big Bend National Park, TX, (28.5N, 104.0W) is centered in this photo. The Rio Grande River bisects the scene; Mexico to the east, USA to the west. The thousand ft. Boquillas limestone cliff on the Mexican side of the river changes colors from white to pink to lavender at sunset. This severely eroded sedimentary landscape was once an ancient seabed later overlaid with volcanic activity.

  16. 33 CFR 80.850 - Brazos River, TX to the Rio Grande, TX.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., San Bernard River, Cedar Lakes, Brown Cedar Cut, Colorado River, Matagorda Bay, Cedar Bayou, Corpus... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Brazos River, TX to the Rio... SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Eighth District § 80.850 Brazos River,...

  17. 33 CFR 80.850 - Brazos River, TX to the Rio Grande, TX.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., San Bernard River, Cedar Lakes, Brown Cedar Cut, Colorado River, Matagorda Bay, Cedar Bayou, Corpus... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Brazos River, TX to the Rio... SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Eighth District § 80.850 Brazos River,...

  18. 33 CFR 80.850 - Brazos River, TX to the Rio Grande, TX.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., San Bernard River, Cedar Lakes, Brown Cedar Cut, Colorado River, Matagorda Bay, Cedar Bayou, Corpus... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Brazos River, TX to the Rio... SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Eighth District § 80.850 Brazos River,...

  19. 33 CFR 80.850 - Brazos River, TX to the Rio Grande, TX.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., San Bernard River, Cedar Lakes, Brown Cedar Cut, Colorado River, Matagorda Bay, Cedar Bayou, Corpus... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Brazos River, TX to the Rio... SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Eighth District § 80.850 Brazos River,...

  20. 33 CFR 80.850 - Brazos River, TX to the Rio Grande, TX.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., San Bernard River, Cedar Lakes, Brown Cedar Cut, Colorado River, Matagorda Bay, Cedar Bayou, Corpus... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Brazos River, TX to the Rio... SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Eighth District § 80.850 Brazos River,...

  1. 78 FR 48298 - Amendment of Class E Airspace; Commerce, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-08

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class E Airspace; Commerce, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action amends Class E airspace at Commerce, TX. Additional controlled airspace is necessary to accommodate new Area Navigation...

  2. 78 FR 48294 - Amendment of Class E Airspace; Mason, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-08

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class E Airspace; Mason, TX AGENCY: Federal... Mason, TX. Additional controlled airspace is necessary to accommodate new Area Navigation (RNAV) Standard Instrument Approach Procedures at Mason County Airport. This action enhances the safety...

  3. 75 FR 11000 - Security Zone; Freeport LNG Basin, Freeport, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-10

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA87 Security Zone; Freeport LNG Basin, Freeport, TX AGENCY... in the Freeport LNG Basin. This security zone is needed to protect vessels, waterfront facilities... notice of proposed rulemaking (NPRM) entitled Security Zone; Freeport LNG Basin, Freeport, TX in...

  4. 76 FR 44254 - Amendment of Class D Airspace; Denton, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-25

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class D Airspace; Denton, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action amends Class D airspace for... proposed rulemaking to amend Class D airspace for Denton, TX, creating additional controlled airspace...

  5. 78 FR 67296 - Establishment of Class D Airspace; Mesquite, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-12

    ... Federal Aviation Administration 14 CFR Part 71 Establishment of Class D Airspace; Mesquite, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action establishes Class D... establish Class D airspace for Mesquite Metro Airport, Mesquite, TX (78 FR 48842) Docket No. FAA-2012-...

  6. 75 FR 55968 - Special Local Regulations, Sabine River; Orange, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-15

    ... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulations, Sabine River; Orange, TX... temporary Special Local Regulation in the Port Arthur Captain of the Port Zone on the Sabine River, Orange... (NPRM) entitled Special Local Regulations; Sabine River, Orange, TX in the Federal Register (75 FR...

  7. 75 FR 41119 - Special Local Regulations; Sabine River, Orange, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-15

    ... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulations; Sabine River, Orange, TX... River, Orange, Texas. This Special Local Regulation is intended to restrict vessels from portions of the..., testing and race in conjunction with the Orange, TX, Thunder on the Sabine boat races. The powerboat...

  8. 76 FR 30890 - Special Local Regulations; Sabine River, Orange, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-27

    ... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulations; Sabine River, Orange, TX... River, Orange, Texas on September 24-25, 2011. This Special Local Regulation is intended to restrict... race in conjunction with the Orange, TX S.P.O.R.T. boat races. The powerboat race and...

  9. 77 FR 46282 - Amendment of Class E Airspace; Sweetwater, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-03

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class E Airspace; Sweetwater, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action amends Class E... Register a notice of proposed rulemaking (NPRM) to amend Class E airspace for the Sweetwater, TX,...

  10. 78 FR 48295 - Amendment of Class E Airspace; Gruver, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-08

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class E Airspace; Gruver, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action amends Class E airspace at... Register a notice of proposed rulemaking (NPRM) to amend Class E airspace for the Gruver, TX,...

  11. 77 FR 56761 - Amendment of Class E Airspace; Kerrville, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-14

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class E Airspace; Kerrville, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action amends Class E... Class E airspace for the Kerrville, TX, area, creating additional controlled airspace at...

  12. 77 FR 66068 - Amendment of Class E Airspace; Breckenridge, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-01

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class E Airspace; Breckenridge, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action amends Class E... Register a notice of proposed rulemaking (NPRM) to amend Class E airspace for the Breckenridge, TX,...

  13. 75 FR 65227 - Revocation of Class E Airspace; Franklin, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-22

    ... Federal Aviation Administration 14 CFR Part 71 Revocation of Class E Airspace; Franklin, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action removes Class E... rulemaking to remove Class E airspace for Franklin, TX (75 FR 36586) Docket No. FAA-2010-0603....

  14. Hanford Single Shell Tank Leak Causes and Locations - 241-TX Farm

    SciTech Connect

    Girardot, C. L.; Harlow, D> G.

    2014-07-22

    This document identifies 241-TX Tank Farm (TX Farm) leak causes and locations for the 100 series leaking tanks (241-TX-107 and 241-TX-114) identified in RPP-RPT-50870, Rev. 0, Hanford 241-TX Farm Leak Inventory Assessment Report. This document satisfies the TX Farm portion of the target (T04) in the Hanford Federal Facility Agreement and Consent Order milestone M-045-91F.

  15. Registration of sorghum germplasm Tx3408 and Tx3409 with tolerance to sugarcane aphid [Melanaphis saccari (Zehntner)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The sorghum (Sorghum bicolor L. Moench) germplasm lines Tx3408 and Tx3409 were developed and released from Texas A&M AgriLife Research and the USDA-ARS in 2015. Both of these lines were developed from intentional crosses using the pedigree method of plant breeding. The breeding crosses for these l...

  16. RadNet Air Data From Fort Worth, TX

    EPA Pesticide Factsheets

    This page presents radiation air monitoring and air filter analysis data for Fort Worth, TX from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

  17. RadNet Air Data From San Angelo, TX

    EPA Pesticide Factsheets

    This page presents radiation air monitoring and air filter analysis data for San Angelo, TX from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

  18. RadNet Air Data From San Antonio, TX

    EPA Pesticide Factsheets

    This page presents radiation air monitoring and air filter analysis data for San Antonio, TX from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

  19. RadNet Air Data From Dallas, TX

    EPA Pesticide Factsheets

    This page presents radiation air monitoring and air filter analysis data for Dallas, TX from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

  20. RadNet Air Data From Houston, TX

    EPA Pesticide Factsheets

    This page presents radiation air monitoring and air filter analysis data for Houston, TX from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

  1. RadNet Air Data From Austin, TX

    EPA Pesticide Factsheets

    This page presents radiation air monitoring and air filter analysis data for Austin, TX from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

  2. RadNet Air Data From Lubbock, TX

    EPA Pesticide Factsheets

    This page presents radiation air monitoring and air filter analysis data for Lubbock, TX from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

  3. RadNet Air Data From El Paso, TX

    EPA Pesticide Factsheets

    This page presents radiation air monitoring and air filter analysis data for El Paso, TX from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

  4. RadNet Air Data From Corpus Christi, TX

    EPA Pesticide Factsheets

    This page presents radiation air monitoring and air filter analysis data for Corpus Christi, TX from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

  5. RadNet Air Data From Harlingen, TX

    EPA Pesticide Factsheets

    This page presents radiation air monitoring and air filter analysis data for Harlingen, TX from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation

  6. RadNet Air Data From Laredo, TX

    EPA Pesticide Factsheets

    This page presents radiation air monitoring and air filter analysis data for Laredo, TX from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

  7. RadNet Air Data From Amarillo, TX

    EPA Pesticide Factsheets

    This page presents radiation air monitoring and air filter analysis data for Amarillo, TX from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

  8. Antibacterial Activity of Ti₃C₂Tx MXene.

    PubMed

    Rasool, Kashif; Helal, Mohamed; Ali, Adnan; Ren, Chang E; Gogotsi, Yury; Mahmoud, Khaled A

    2016-03-22

    MXenes are a family of atomically thin, two-dimensional (2D) transition metal carbides and carbonitrides with many attractive properties. Two-dimensional Ti3C2Tx (MXene) has been recently explored for applications in water desalination/purification membranes. A major success indicator for any water treatment membrane is the resistance to biofouling. To validate this and to understand better the health and environmental impacts of the new 2D carbides, we investigated the antibacterial properties of single- and few-layer Ti3C2Tx MXene flakes in colloidal solution. The antibacterial properties of Ti3C2Tx were tested against Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis) by using bacterial growth curves based on optical densities (OD) and colonies growth on agar nutritive plates. Ti3C2Tx shows a higher antibacterial efficiency toward both Gram-negative E. coli and Gram-positive B. subtilis compared with graphene oxide (GO), which has been widely reported as an antibacterial agent. Concentration dependent antibacterial activity was observed and more than 98% bacterial cell viability loss was found at 200 μg/mL Ti3C2Tx for both bacterial cells within 4 h of exposure, as confirmed by colony forming unit (CFU) and regrowth curve. Antibacterial mechanism investigation by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) coupled with lactate dehydrogenase (LDH) release assay indicated the damage to the cell membrane, which resulted in release of cytoplasmic materials from the bacterial cells. Reactive oxygen species (ROS) dependent and independent stress induction by Ti3C2Tx was investigated in two separate abiotic assays. MXenes are expected to be resistant to biofouling and offer bactericidal properties.

  9. 75 FR 41983 - Establishment of Class D Airspace; San Marcos, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-20

    ... airspace for San Marcos Municipal Airport, San Marcos, TX. Establishment of an air traffic control tower... establishing Class D airspace at San Marcos, TX. Establishment of an air traffic control tower at San...

  10. 76 FR 53354 - Proposed Amendment of Class E Airspace; Winters, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-26

    ... business hours at the office of the Central Service Center, 2601 Meacham Blvd, Fort Worth, TX 76137... airspace areas extending upward from 700 feet or more above the surface of the earth. * * * * * ASW TX...

  11. 76 FR 28060 - Regional Habitat Conservation Plan, Hays County, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-13

    ... Fish and Wildlife Service Regional Habitat Conservation Plan, Hays County, TX AGENCY: Fish and Wildlife... statement, final Hays County regional habitat conservation plan, and draft record of decision. SUMMARY: We... (EIS), the final Hays County regional habitat conservation plan (RHCP) under the National...

  12. 77 FR 58800 - Radio Broadcasting Services; Knox City, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-24

    ... COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Knox City, TX AGENCY: Federal Communications... Allotments by substituting Channel 277A for vacant Channel 291A, at Knox City, Texas. The proposal is part of... at Knox City, Texas, in compliance with the Commission's minimum distance separation requirements...

  13. 76 FR 5120 - Television Broadcasting Services; El Paso, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-28

    ... COMMISSION 47 CFR Part 73 Television Broadcasting Services; El Paso, TX AGENCY: Federal Communications... Comcorp of El Paso License Corp. the licensee of station KTSM-TV, channel 9, El Paso, Texas, requesting the substitution of channel 16 for channel 9 at El Paso. DATES: Comments must be filed on or...

  14. 76 FR 19276 - Television Broadcasting Services; El Paso, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-07

    ... COMMISSION 47 CFR Part 73 Television Broadcasting Services; El Paso, TX AGENCY: Federal Communications... to a petition for rulemaking filed by Comcorp of El Paso License Corp. (``Comcorp''), the licensee of KTSM-TV, channel 9, El Paso, Texas, requesting the substitution of channel 16 for channel 9 at El...

  15. 76 FR 61135 - Environmental Impact Statement: El Paso County, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-03

    ... Federal Highway Administration Environmental Impact Statement: El Paso County, TX AGENCY: Federal Highway... proposed ] improvements to Loop 375 Border Highway West, in El Paso County, Texas, is being rescinded. FOR... Loop 375 C sar Ch vez Highway (Border Highway West Extension) in El Paso, Texas, to include the...

  16. 76 FR 44280 - Television Broadcasting Services; El Paso, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-25

    ... COMMISSION 47 CFR Part 73 Television Broadcasting Services; El Paso, TX AGENCY: Federal Communications...''), licensee of KVIA-TV, channel 7, El Paso, Texas, requesting the substitution of channel 17 for channel 7 at El Paso. KVIA-TV has experienced extensive signal coverage problems on channel 7 following the...

  17. 76 FR 28946 - Television Broadcasting Services; El Paso, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-19

    ... COMMISSION 47 CFR Part 73 Television Broadcasting Services; El Paso, TX AGENCY: Federal Communications... NPG of Texas, LP (``NPG''), licensee of station KVIA-TV, El Paso, Texas, requesting the substitution of channel 17 for channel 7 at El Paso. NPG states that the proposed channel substitution will...

  18. 77 FR 32900 - Radio Broadcasting Services; Llano, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-04

    ... COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Llano, TX AGENCY: Federal Communications Commission... Congressional Review Act, see U.S.C. 801(a)(1)(A). List of Subjects in 47 CFR Part 73 Radio, Radio broadcasting...: PART 73--RADIO BROADCAST SERVICES 0 1. The authority citation for part 73 continues to read as...

  19. 76 FR 13579 - Radio Broadcasting Services; Hebbronville, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-14

    ... COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Hebbronville, TX AGENCY: Federal Communications... filing procedures for comments, see 47 CFR 1.415 and 1.420. List of Subjects in 47 CFR Part 73 Radio, Radio broadcasting. For the reasons discussed in the preamble, the Federal Communications...

  20. 78 FR 4078 - Radio Broadcasting Services; Hebbronville, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-18

    ... COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Hebbronville, TX AGENCY: Federal Communications... Review Act, see 5 U.S.C. 801(a)(1)(A). List of Subjects in 47 CFR Part 73 Radio, Radio broadcasting...: PART 73--RADIO BROADCAST SERVICES 0 1. The authority citation for part 73 continues to read as...

  1. 75 FR 67806 - Environmental Impact Statement; Nueces County, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-03

    ... Federal Highway Administration Environmental Impact Statement; Nueces County, TX AGENCY: Federal Highway... proposed improvements to United States Highway 181/State Highway 286 (Crosstown Expressway), in Nueces... Nueces County, Texas. The project limits were defined as the limits of the schematic design. The...

  2. 75 FR 8489 - Security Zone; Freeport Channel Entrance, Freeport, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-25

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA87 Security Zone; Freeport Channel Entrance, Freeport, TX AGENCY: Coast Guard, DHS. ACTION: Final rule. SUMMARY: The Coast Guard has established moving security zones for certain vessels, for which the Captain of the Port, Houston-Galveston deems enhanced...

  3. 75 FR 8491 - Security Zones; Brazos River, Freeport, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-25

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA87 Security Zones; Brazos River, Freeport, TX AGENCY: Coast Guard, DHS. ACTION: Final rule. SUMMARY: The Coast Guard has established four permanent security zones in the Brazos River in Freeport, Texas. These security zones are being put in place to...

  4. 75 FR 9859 - Television Broadcasting Services; Beaumont, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-04

    ... COMMISSION 47 CFR Part 73 Television Broadcasting Services; Beaumont, TX AGENCY: Federal Communications... Freedom Broadcasting of Texas (``Freedom Broadcasting''), the licensee of KFDM(TV), channel 21, Beaumont, Texas. Freedom Broadcasting requests ] the substitution of channel 25 for channel 21 at Beaumont....

  5. 75 FR 19907 - Television Broadcasting Services; Beaumont, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-16

    ... COMMISSION 47 CFR Part 73 Television Broadcasting Services; Beaumont, TX AGENCY: Federal Communications... Broadcasting of Texas, the licensee of KFDM(TV), channel 21, Beaumont, Texas, requesting the substitution of... broadcasting. 0 For the reasons discussed in the preamble, the Federal Communications Commission amends 47...

  6. 75 FR 4037 - FM TABLE OF ALLOTMENTS, BRACKETTVILLE, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-26

    ... COMMISSION 47 CFR Part 73 FM TABLE OF ALLOTMENTS, BRACKETTVILLE, TX AGENCY: Federal Communications Commission... proposed deletion of this vacant allotment accommodates the new FM station application, which requests the... Rocksprings, to Brackettville, Texas, modification of the new FM station authorization. See File No....

  7. 78 FR 21337 - Radio Broadcasting Services; Matagorda, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-10

    ... COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Matagorda, TX AGENCY: Federal Communications... filed by Tejas Broadcasting Ltd., LLP (``Petitioner''), licensee of FM Station KMJR, Channel 252C2, Odem, Texas. Petitioner proposes to amend the FM Table of Allotments by substituting Channel 291A for...

  8. 76 FR 36384 - Radio Broadcasting Services; Brackettville, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-22

    ... COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Brackettville, TX AGENCY: Federal Communications... request of RF Services, Inc., licensee of a new FM station at Rocksprings, Texas, that requests the... Rocksprings, to Brackettville, Texas, and modification of the new FM station authorization. See File No....

  9. 75 FR 18776 - Regulated Navigation Area; Galveston Channel, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-13

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA11 Regulated Navigation Area; Galveston Channel, TX AGENCY... a regulated navigation area across the entire width of the Galveston Channel in the vicinity of Sector Field Office (SFO) Galveston. This regulated navigation area would require vessels to navigate...

  10. 75 FR 47713 - Regulated Navigation Area; Galveston Channel, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-09

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA11 Regulated Navigation Area; Galveston Channel, TX AGENCY: Coast Guard, DHS. ACTION: Final rule. SUMMARY: The Coast Guard is establishing a regulated navigation... Navigation Area; Galveston Channel'' in the Federal Register (75 FR 18766). We received no comments on...

  11. 75 FR 55618 - Texas Disaster Number TX-00362

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-13

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Texas Disaster Number TX-00362 AGENCY: U.S. Small Business Administration. ACTION: Amendment 4... following areas as adversely affected by the disaster. Primary Counties: Calhoun. All other information...

  12. 76 FR 70528 - Texas Disaster Number TX-00382

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-14

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Texas Disaster Number TX-00382 AGENCY: U.S. Small Business Administration. ACTION: Amendment 5... following areas as adversely affected by the disaster. Primary Counties: Briscoe, Clay, Coryell,...

  13. 76 FR 59480 - Texas Disaster Number TX-00381

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-26

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Texas Disaster Number TX-00381 AGENCY: U.S. Small Business Administration. ACTION: Amendment 2... areas as adversely affected by the disaster: Primary Counties: (Physical Damage and Economic...

  14. 76 FR 59766 - Texas Disaster Number TX-00381

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-27

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Texas Disaster Number TX-00381 AGENCY: U.S. Small Business Administration. ACTION: Amendment 3... areas as adversely affected by the disaster: Primary Counties: (Physical Damage and Economic...

  15. 78 FR 18801 - Establishment of Class E Airspace; Beeville, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-28

    ... Federal Aviation Administration 14 CFR Part 71 Establishment of Class E Airspace; Beeville, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action establishes Class E... Federal Register a notice of proposed rulemaking (NPRM) to establish Class E airspace at Chase...

  16. 78 FR 61179 - Establishment of Class E Airspace; Comanche, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-03

    ... Federal Aviation Administration 14 CFR Part 71 Establishment of Class E Airspace; Comanche, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action establishes Class E... established for the airport in 2006, but the Class E airspace area to contain it was never...

  17. 75 FR 13668 - Amendment of Class E Airspace; Georgetown, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-23

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class E Airspace; Georgetown, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action amends Class E... Federal Register a notice of proposed rulemaking to amend Class E airspace for Georgetown...

  18. 76 FR 53352 - Proposed Amendment of Class E Airspace; Alice, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-26

    ... instrument approach procedures at Old Hoppe Place Airport, Agua Dulce, TX, has made this action necessary for..., area. Controlled airspace extending upward from 700 feet above the surface is being removed at Old... feet or more above the surface of the earth. * * * * * ASW TX E5 Alice, TX Alice International...

  19. 77 FR 71367 - Proposed Establishment of Class E Airspace; Round Mountain, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-30

    ... Mountain, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of proposed rulemaking (NPRM). SUMMARY: This action proposes to establish Class E airspace at Round Mountain, TX. Controlled... instrument approach procedures at West Ranch Airport, Round Mountain, TX. Controlled airspace is needed...

  20. 78 FR 19097 - Establishment of Class E Airspace; Round Mountain, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-29

    ... Federal Aviation Administration 14 CFR Part 71 Establishment of Class E Airspace; Round Mountain, TX... Class E airspace at Round Mountain, TX. Controlled airspace is necessary to accommodate new Area... the West Ranch Airport, Round Mountain, TX (77 FR 71367) Docket No. FAA-2012-0771. Interested...

  1. 75 FR 70945 - Caddo National Wildlife Refuge, Harrison County, TX; Comprehensive Conservation Plan and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-19

    ... Fish and Wildlife Service Caddo National Wildlife Refuge, Harrison County, TX; Comprehensive... Wildlife Refuge, Harrison County, TX. We provide this notice in compliance with our CCP policy to advise... Lake NWR (Refuge), located in Harrison County, TX. This notice complies with our CCP policy to...

  2. 76 FR 5303 - Proposed Amendment of Class E Airspace; Carrizo Springs, Glass Ranch Airport, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-31

    ..., Glass Ranch Airport, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of proposed rulemaking (NPRM). SUMMARY: This action proposes to amend Class E airspace for the Carrizo Springs, Glass... Carrizo Springs, Glass Ranch Airport, TX Carrizo Springs, Glass Ranch Airport, TX (Lat. 28 27'01''...

  3. 75 FR 81442 - Revocation of Class E Airspace; Lone Star, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-28

    ... Federal Aviation Administration 14 CFR Part 71 Revocation of Class E Airspace; Lone Star, TX AGENCY... airspace at Lone Star, TX. Abandonment of the former Lone Star Steel Company ] Airport and cancellation of... the Lone Star, TX, area. The FAA is taking this action to ensure the efficient use of airspace...

  4. 75 FR 64972 - Proposed Revocation of Class E Airspace; Lone Star, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-21

    ... Federal Aviation Administration 14 CFR Part 71 Proposed Revocation of Class E Airspace; Lone Star, TX...: This action proposes to remove Class E airspace at Lone Star, TX. Abandonment of the former Lone Star... eliminated the need for controlled airspace in the Lone Star, TX, area. The FAA is taking this action...

  5. 75 FR 6319 - Proposed Amendment of Low Altitude Area Navigation Route T-254; Houston, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-09

    ... altitude Area Navigation (RNAV) route T-254 in the Houston, TX, terminal area by eliminating the segment... safety and the efficient use of the navigable airspace in the Houston, TX, terminal area. DATES: Comments... amend the low altitude RNAV route T-254 in the Houston, TX, terminal area by eliminating the...

  6. 77 FR 4700 - Proposed Establishment of Class E Airspace; Freer, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-31

    ... Federal Aviation Administration 14 CFR Part 71 Proposed Establishment of Class E Airspace; Freer, TX...: This action proposes to establish Class E airspace at Freer, TX. Controlled airspace is necessary to... approach procedures at Seven C's Ranch Airport, Freer, TX. Controlled airspace is needed for the safety...

  7. 76 FR 31821 - Amendment of Class D Airspace; Corpus Christi, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-02

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class D Airspace; Corpus Christi, TX AGENCY... amends Class D airspace within the Corpus Christi, TX, area by updating the geographic coordinates for... the geographic coordinates, within Class D airspace, of the Cabaniss NOLF, Corpus Christi, TX,...

  8. 75 FR 18047 - Amendment of Low Altitude Area Navigation Route T-254; Houston, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-09

    ... Route T-254; Houston, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action amends low altitude Area Navigation (RNAV) route T-254 in the Houston, TX, terminal area by... the College Station, TX, VORTAC. The FAA is taking this action to eliminate a portion of T-254 that...

  9. 75 FR 39149 - Establishment of Low Altitude Area Navigation Route (T-284); Houston, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-08

    ... Route (T-284); Houston, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule... area navigation (RNAV) route T-284 for the Houston, TX, terminal area, is being withdrawn. As a result... Federal Register a final rule to establish RNAV route T-284 for the Houston, TX, terminal area (75...

  10. RXTE monitoring of the intermediate polar TX Col

    NASA Astrophysics Data System (ADS)

    Wheatley, Peter J.

    I present a preliminary analysis of ongoing monthly X-ray observations of TX Col with RXTE. The lightcurves confirm that the relative strength of spin and beat modulations is highly variable in this system, and show that the timescale of variation is shorter than one month. Changes in these modulations are thought to represent a change in accretion geometry, possibly governed by a varying accretion rate. I find some evidence that the ratio of spin to beat amplitudes is correlated with the mean count rate.

  11. Staubli TX-90XL robot qualification at the LLIHE.

    SciTech Connect

    Covert, Timothy Todd

    2010-10-01

    The Light Initiated High Explosive (LIHE) Facility uses a robotic arm to spray explosive material onto test items for impulse tests. In 2007, the decision was made to replace the existing PUMA 760 robot with the Staubli TX-90XL. A qualification plan was developed and implemented to verify the safe operating conditions and failure modes of the new system. The robot satisfied the safety requirements established in the qualification plan. A performance issue described in this report remains unresolved at the time of this publication. The final readiness review concluded the qualification of this robot at the LIHE facility.

  12. Decision support system for individualized nursing procedures: SAPIEN-Tx.

    PubMed

    Ito, M; Ramos, M P; Chern, M S; Espósito, S R; Carmagnani, M I; Cunha, I C; Piveta, V M; Nespoulos, E; Iwasa, A T; Anção, M S

    1995-01-01

    The present work proposes a Decision Support System for nursing procedures: SAPIEN-Tx. The discussion includes the acquisition, modeling , and implementation of nursing expertise professionals in Renal Transplant. It was developed to obtain better quality healthcare services, as well as an effective contribution to the nursing professional in the global assistance of their clientele. We used the KADS methodology to develop the system knowledge base. This methodology permitted us to perform the knowledge modeling with quality and organization. In opposition to the old method, errors were detected before the implementation, avoiding possible modification on the whole project structure.

  13. 60-day waste compatibility safety issues and final results for TX-244 grab samples

    SciTech Connect

    Nuzum, J.L., Fluor Daniel Hanford

    1997-02-05

    Three grab samples (244-TX-96-1, 244-TX-96-2, and 244-TX-96-3) were taken from Riser 8 of Tank 241-TX-244 on October 18, 1996, and received by 222-S Laboratory on October 18, 1996. These samples were analyzed in accordance with Compatibility Grab Sampling and Analysis Plan (TSAP) and Data Quality Objectives for Tank Farms Waste Compatibility Program (DQO) in support ofthe Waste Compatibility Program. Notifications were made in accordance with TSAP for pH and OH- analyses. Upon further review, the pH notification was deemed unnecessary, as the notification limit did not apply to this tank.

  14. 77 FR 74170 - Foreign-Trade Zone 84-Houston, TX; Notification of Proposed Production Activity; Mitsubishi...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-13

    ...; Mitsubishi Caterpillar Forklift America Inc.; (Forklift Trucks); Houston, TX The Port of Houston Authority... Caterpillar Forklift America Inc. (MCFA), located in Houston, Texas. The notification conforming to...

  15. TX Cnc AS A MEMBER OF THE PRAESEPE OPEN CLUSTER

    SciTech Connect

    Zhang, X. B.; Deng, L.; Lu, P.

    2009-08-15

    We present B-, V-, and I-band CCD photometry of the W UMa-type binary system TX Cnc, which is a member star of the Praesepe open cluster. Based on the observations, new ephemeris and a revised photometric solution of the binary system were derived. Combined with the results of the radial velocity solution contributed by Pribulla et al., the absolute parameters of the system were determined. The mass, radius, and luminosity of the primary component are derived to be 1.35 {+-} 0.02 M {sub sun}, 1.27 {+-} 0.04 R {sub sun}, and 2.13 {+-} 0.11 L {sub sun}. Those for the secondary star are computed as 0.61 {+-} 0.01 M {sub sun}, 0.89 {+-} 0.03 R {sub sun}, and 1.26 {+-} 0.07 L {sub sun}, respectively. Based on these results, a distance modulus of (m - M) {sub V} = 6.34 {+-} 0.05 is determined for the star. It confirms the membership of TX Cnc to the Praesepe open cluster. The evolutionary status and the physical nature of the binary system are discussed compared with the theoretical model.

  16. Modal testing of the TX-100 wind turbine blade.

    SciTech Connect

    Reese, Sarah; Griffith, Daniel Todd; Casias, Miguel; Simmermacher, Todd William; Smith, Gregory A.

    2006-05-01

    This test report covers the SNL modal test results for two nominally identical TX-100 wind turbine blades. The TX-100 blade design is unique in that it features a passive braking, force-shedding mechanism where bending and torsion are coupled to produce desirable aerodynamic characteristics. A specific aim of this test is to characterize the coupling between bending and torsional dynamics. The results of the modal tests and the subsequent analysis characterize the natural frequencies, damping, and mode shapes of the individual blades. The results of this report are expected to be used for model validation--the frequencies and mode shapes from the experimental analysis can be compared with those of a finite-element analysis. Damping values are included in the results of these tests to potentially improve the fidelity of numerical simulations, although numerical finite element models typically have no means of predicting structural damping characteristics. Thereafter, an additional objective of the test is achieved in evaluating the test to test and unit variation in the modal parameters of the two blades.

  17. 78 FR 33019 - Proposed Amendment of Class E Airspace; Commerce, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-03

    ... Federal Aviation Administration 14 CFR Part 71 Proposed Amendment of Class E Airspace; Commerce, TX AGENCY... action proposes to amend Class E airspace at Commerce, TX. Additional controlled airspace is necessary to accommodate new Standard Instrument Approach Procedures (SIAPs) at Commerce Municipal Airport (AAF). The...

  18. 76 FR 61687 - City of College Station, TX; Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-05

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission City of College Station, TX; Notice of Filing Take notice that on September... City of College Station, TX filed an application requesting that the Commission direct (1)...

  19. 77 FR 40897 - Notice of Intent to Repatriate Cultural Items: Gregg County Historical Museum, Longview, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-11

    ... burials at the E. Fields site (no site trinomial has been assigned), in Harrison County, TX. The exact... funerary objects were removed from the HC site (no site trinomial has been assigned), in Harrison County... assigned), in Harrison County, TX. The exact location of this site is not specified in notes or records...

  20. 75 FR 15360 - Proposed Amendment of Class E Airspace; Austin, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-29

    ... Federal Aviation Administration 14 CFR Part 71 Proposed Amendment of Class E Airspace; Austin, TX AGENCY... action proposes to amend Class E airspace in the Austin, TX area. Additional controlled airspace is necessary to accommodate new Standard Instrument Approach Procedures (SIAPs) at Austin Executive...

  1. 78 FR 57545 - Proposed Amendment of Class D Airspace; Dallas, Addison Airport, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-19

    ... Airport, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of proposed rulemaking... Class D airspace ceiling at Addison Airport, Dallas, TX, is being withdrawn. Upon review, the FAA... 6, 2013, a NPRM was published in the Federal Register proposing to amend the Addison Airport Class...

  2. 76 FR 28888 - Revocation of Class E Airspace; Gruver Cluck Ranch Airport, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-19

    ... Federal Aviation Administration 14 CFR Part 71 Revocation of Class E Airspace; Gruver Cluck Ranch Airport... removes Class E airspace at Gruver, Cluck Ranch Airport, TX. The airport has been abandoned, thereby eliminating the need for controlled airspace in the Gruver, Cluck Ranch Airport, TX, area. The FAA is...

  3. 75 FR 43886 - Proposed Amendment of Class E Airspace; Corpus Christi, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-27

    ..., Nueces County Airport, TX (Lat. 27 46'43'' N., long. 97 41'26'' W.) Corpus Christi VORTAC, TX (Lat. 27 54... within a 6.3-mile radius of Nueces County Airport, and within a 7.6-mile radius of Aransas County...

  4. 75 FR 13453 - Proposed Amendment of Class E Airspace; Corpus Christi, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-22

    ..., Nueces County Airport, TX (Lat. 27 46'43'' N., long. 97 41'26'' W.) Corpus Christi VORTAC, TX (Lat. 27 54...Campbell Airport, and within a 6.3-mile radius of Nueces County Airport, and within a 7.6-mile radius...

  5. 76 FR 22011 - Amendment of Class E Airspace; Carizzo Springs, Glass Ranch Airport, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-20

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class E Airspace; Carizzo Springs, Glass Ranch... amends Class E airspace for the Carizzo Springs, Glass Ranch Airport, TX, airspace area, to accommodate... rulemaking to amend Class E airspace for the Carizzo Springs, Glass Ranch Airport, TX, airspace...

  6. 75 FR 16336 - Establishment of Low Altitude Area Navigation Route (T-284); Houston, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-01

    ... Houston, TX, terminal area, to expedite the handling of Instrument Flight Rules (IFR) overflight aircraft transitioning busy terminal airspace. The FAA is taking this action to enhance the safe and efficient use of the navigable airspace in the Houston, TX, terminal area. DATES: Effective date 0901 UTC, July 29, 2010....

  7. 75 FR 8742 - Notice of Inventory Completion: Stephen F. Austin State University, Nacogdoches, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-25

    ... individual were removed from an airport west of Tyler in Smith County, TX, by ``Red'' McFarland, a private... Kickapoo River, Smith County, TX, by ``Red'' McFarland, a private citizen. The human remains are located in... marine shell beads and fragments; 1 fragmented marine shell pendant; 3 deer teeth; 9 pigment samples;...

  8. 76 FR 49285 - Amendment of Class D Airspace; Corpus Christi, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-10

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class D Airspace; Corpus Christi, TX AGENCY... Register June 2, 2011, ] amending Class D airspace at Cabaniss Navy Outlying Field (NOLF), Corpus Christi... Cabaniss NOLF, Corpus Christi, TX (76 FR 31821, Docket No. FAA-2010-1171). Subsequent to publication,...

  9. Registration of perennial Sorghum bicolor x S. propinquum line 'PSH12TX09'

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A novel sorghum x Sorghum propinquum recombinant inbred line, named PSSH12TX09, was identified that successfully overwintered across plant hardiness zones 8a, 8b, and 9a between 2013 and 2015. Overwintering perenniality of PSSH12TX09 exceeded 90% at all locations across both evaluation years, with ...

  10. 78 FR 31429 - Proposed Amendment of Class E Airspace; Mason, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-24

    ...; Mason, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of proposed rulemaking (NPRM). SUMMARY: This action proposes to amend Class E airspace at Mason, TX. Additional controlled airspace is necessary to accommodate new Standard Instrument Approach Procedures (SIAP) at Mason...

  11. 77 FR 47519 - Annual Marine Events in the Eighth Coast Guard District, Sabine River; Orange, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-09

    ... SECURITY Coast Guard 33 CFR Part 100 Annual Marine Events in the Eighth Coast Guard District, Sabine River; Orange, TX AGENCY: Coast Guard, DHS. ACTION: Notice of enforcement of regulation. SUMMARY: The Coast..., call or email Mr. Scott Whalen. U.S. Coast Guard Marine Safety Unit Port Arthur, TX; telephone...

  12. 75 FR 58339 - Prevailing Rate Systems; Redefinition of the Shreveport, LA; Texarkana, TX; Milwaukee, WI; and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-24

    ..., TX, comprise the Longview, TX MSA. The Longview MSA is currently split between the Shreveport, LA... on an analysis of the regulatory criteria for Gregg County, the core county in the Longview MSA, we recommend that the entire Longview MSA be defined to the Shreveport area of application. The...

  13. 75 FR 66303 - Revocation of Restricted Areas R-3807 Glencoe, LA, and R-6320 Matagorda, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-28

    ... Federal Aviation Administration 14 CFR Part 73 RIN 2120-AA66 Revocation of Restricted Areas R-3807 Glencoe, LA, and R-6320 Matagorda, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action removes Restricted Areas R-3807 Glencoe, LA, and R-6320 Matagorda, TX....

  14. 76 FR 1513 - Amendment of Class E Airspace; Horseshoe Bay, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-11

    ... airspace for Horseshoe Bay, TX. Decommissioning of the Horseshoe Bay Resort non-directional beacon (NDB) at Horseshoe Bay Resort Airport, Horseshoe Bay, TX, has made this action necessary to enhance the safety and management of Instrument Flight Rule (IFR) operations at the airport. DATES: Effective date 0901 UTC,...

  15. 78 FR 28801 - Foreign-Trade Zone 117-Orange, TX, Authorization of Production Activity, Signal International...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-16

    ... Foreign-Trade Zones Board Foreign-Trade Zone 117--Orange, TX, Authorization of Production Activity, Signal International Texas GP, LLC (Shipbuilding), Orange, TX On January 10, 2013, the Foreign Trade Zone of Southeast...-Trade Zones (FTZ) Board on behalf of Signal International Texas GP, LLC, in Orange, Texas....

  16. Photometry Of The Semi-regular Variable Tx Tau

    NASA Astrophysics Data System (ADS)

    Wyman, Katherine; Spear, G.; McLin, K.; Cominsky, L.; Mankiewicz, L.; Reichart, D.; Ivarsen, K.

    2009-12-01

    We report V-band and I-band photometry for the SRA type variable TX Tau. Photometry was obtained using the robotic telescope GORT at the Hume Observatory (NASA funded through Sonoma State University) and the PROMPT robotic telescopes at Cerro Tololo Inter-American Observatory (NSF and NASA funded through the University of North Carolina). Photometry was also obtained using the PI of the Sky optical transient search system at Las Campanas Observatory developed by a consortium of institutions in Poland. Modern periods are determined, the V-band and I-band light curves are compared, and V-I colors are derived. It is possible that the classification for this variable should be reconsidered.

  17. Mass flow in the interacting binary TX Ursae Majoris

    NASA Technical Reports Server (NTRS)

    Mccluskey, G. E., Jr.; Mccluskey, C. P. S.; Kondo, Y.

    1988-01-01

    Twenty-two far-ultraviolet and 23 near-ultraviolet high resolution IUE spectra of the interactive Algol-type binary TX Ursae Majoris (B8 V + F-K III-IV) were analyzed in order to determine the nature of the mass flow occurring in this system. Absorption features due to high-temperature ions of Si IV, C IV, and N V are always present. The resonance lines of Al III, Fe II, Mg II and Si IV show strong phase and secular variations indicative of gas streaming and circumstellar/circumbinary material. Radial velocities as high as 500 to 600 km/sec are present. The gas flow is particularly prominent in 1985 between phases 0.7 and 0.0. The system is more active than U Sagittae and as active as U Cephei.

  18. CX-100 and TX-100 blade field tests.

    SciTech Connect

    Holman, Adam (USDA-Agriculture Research Service, Bushland, TX); Jones, Perry L.; Zayas, Jose R.

    2005-12-01

    In support of the DOE Low Wind Speed Turbine (LWST) program two of the three Micon 65/13M wind turbines at the USDA Agricultural Research Service (ARS) center in Bushland, Texas will be used to test two sets of experimental blades, the CX-100 and TX-100. The blade aerodynamic and structural characterization, meteorological inflow and wind turbine structural response will be monitored with an array of 75 instruments: 33 to characterize the blades, 15 to characterize the inflow, and 27 to characterize the time-varying state of the turbine. For both tests, data will be sampled at a rate of 30 Hz using the ATLAS II (Accurate GPS Time-Linked Data Acquisition System) data acquisition system. The system features a time-synchronized continuous data stream and telemetered data from the turbine rotor. This paper documents the instruments and infrastructure that have been developed to monitor these blades, turbines and inflow.

  19. Chemical Contamination of the Lower Rio Grande near Laredo, TX

    NASA Astrophysics Data System (ADS)

    Flores, B.; Ren, J.; Krishnamurthy, S.; Belzer, W.

    2006-12-01

    The Rio Grande River stretches over 2000 miles from the southern Rocky Mountains in Colorado to the tip of Texas where the Rio Grande meets the Gulf of Mexico. It is the natural boundary between U.S. and Mexico from El Paso, TX, to Brownsville, TX. The communities along the border heavily rely upon the Rio Grande as a primary source of water for consumption, agricultural uses, supporting wildlife and recreation. For many years the Rio Grande has been polluted with municipal, industrial, agricultural and farming contaminants from both sides of the border. This pollution has led to the extinction or reduction of certain wildlife species as well as affecting the health of the residences along the border. Even though great strides have been made in monitoring the Rio Grande, there has been a lack of intense monitoring data collection for pollutants such as pesticides. Three sampling sites including Manadas Creek, the Rio Grande River at International Bridge I, and USGS monitoring site 08459200 off of Highway 83 were chosen. The water quality parameters focused include temperature, pH, conductivity, dissolve oxygen (DO), salinity, total dissolved solids, nutrients, metals and pesticides. Preliminary results have shown elevated concentration of total phosphorus and ortho-phosphorus in the Manadas Creek site. Organochlorinated pesticides such as heptachlor and 4, 4 DDE were detected at various concentrations at all sites and endrin aldehyde was found at Manadas Creek site. This research has provided more information on the current chemical contamination level of the Rio Grande in the Laredo area.

  20. Backwater Flooding in San Marcos, TX from the Blanco River

    NASA Technical Reports Server (NTRS)

    Earl, Richard; Gaenzle, Kyle G.; Hollier, Andi B.

    2016-01-01

    Large sections of San Marcos, TX were flooded in Oct. 1998, May 2015, and Oct. 2015. Much of the flooding in Oct. 1998 and Oct. 2015 was produced by overbank flooding of San Marcos River and its tributaries by spills from upstream dams. The May 2015 flooding was almost entirely produced by backwater flooding from the Blanco River whose confluence is approximately 2.2 miles southeast of downtown. We use the stage height of the Blanco River to generate maps of the areas of San Marcos that are lower than the flood peaks and compare those results with data for the observed extent of flooding in San Marcos. Our preliminary results suggest that the flooding occurred at locations more than 20 feet lower than the maximum stage height of the Blanco River at San Marcos gage (08171350). This suggest that the datum for either gage 08171350 or 08170500 (San Marcos River at San Marcos) or both are incorrect. There are plans for the U.S. Army Corps of Engineers to construct a Blanco River bypass that will divert Blanco River floodwaters approximately 2 miles farther downstream, but the $60 million price makes its implementation problematic.

  1. TX-100/water/1-butyl-3-methylimidazolium hexafluorophosphate microemulsions.

    PubMed

    Gao, Yanan; Han, Shuaibing; Han, Buxing; Li, Ganzuo; Shen, Dong; Li, Zhonghao; Du, Jimin; Hou, Wanguo; Zhang, Gaoyong

    2005-06-21

    Both ionic liquids and water are typical green solvents. In this work, the phase behavior of the ternary system consisting of ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate (bmimPF6), TX-100, and water was determined at 25.0 degrees C. The water-in-bmimPF6, bicontinuous, and bmimPF6-in-water microregions of the microemulsions were identified by cyclic voltammetry method using potassium ferrocyanide K4Fe(CN)6 as the electroactive probe. Dynamic light scattering (DLS) and the UV-vis method were used to characterize the microemulsions. It was demonstrated that the hydrodynamic diameter (Dh) of the bmimPF6-in-water microemulsions is nearly independent of the water content but increases with increasing bmimPF6 content due to the swelling of the micelles by the ionic liquid. The UV-vis further confirmed the existence of water domains in the water-in-bmimPF6 microemulsions, and the salt potassium ferricyanide K3Fe(CN)6 could be dissolved in the water domains.

  2. ORNL measurements at Hanford Waste Tank TX-118

    SciTech Connect

    Koehler, P.E.; Mihalczo, J.T.

    1995-02-01

    A program of measurements and calculations to develop a method of measuring the fissionable material content of the large waste storage tanks at the Hanford, Washington, site is described in this report. These tanks contain radioactive waste from the processing of irradiated fuel elements from the plutonium-producing nuclear reactors at the Hanford site. Time correlation and noise analysis techniques, similar to those developed for and used in the Nuclear Weapons Identification System at the Y-12 Plant in Oak Ridge, Tennessee, will be used at the Hanford site. Both ``passive`` techniques to detect the neutrons emitted spontaneously from the waste in the tank and ``active`` techniques using AmBe and {sup 252}Cf neutron sources to induce fissions will be used. This work is divided into three major tasks: (1) development of high-sensitivity neutron detectors that can selectively count only neutrons in the high {gamma} radiation fields in the tanks, (2) Monte Carlo neutron transport calculations using both the KENO and MCNP codes to plan and analyze the measurements, and (3) the measurement of time-correlated neutrons by time and frequency analysis to distinguish spontaneous fission from sources inside the tanks. This report describes the development of the detector and its testing in radiation fields at the Radiation Calibration Facility at Oak Ridge National Laboratory and in tank TX-118 at the 200 W area at Westinghouse Hanford Company.

  3. 75 FR 15992 - Revision of Prohibited Area P-49; Crawford, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-31

    ... amends Prohibited Area 49 (P-49) Crawford, TX. While the United States Secret Service (USSS) recognizes... designation. Continuous. Using agency. United States Secret Service, Washington, DC. Issued in Washington,...

  4. E3 Success Story - Reducing Electrical Demand in San Antonio, TX

    EPA Pesticide Factsheets

    To meet its goal of reducing electrical demand by 9 megawatts CPS Energy in San Antonio, TX partnered with the Texas Manufacturing Assistance Center (TMAC) and the Southwest Research Institute to provide lean, clean and energy efficiency training.

  5. 75 FR 22712 - Proposed Establishment of Class D Airspace; San Marcos, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-30

    ... control tower has made controlled airspace necessary at San Marcos Municipal Airport. The FAA is taking... establishing Class D airspace at San Marcos Municipal Airport, San Marcos, TX. An air traffic control...

  6. 76 FR 30396 - Deloitte Financial Advisory Services LLP, Real Estate Consulting, Houston, TX; Amended...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-25

    ... Financial Advisory Services LLP, Real Estate Consulting, Houston, TX; Amended Certification Regarding... of Deloitte Financial Advisory Services LLP, Real Estate Consulting, Houston, Texas (subject firm... subject worker group supplies real estate valuation services for the subject firm. The Department, on...

  7. 76 FR 12788 - Environmental Impact Statement for a Proposed Urban Rail system in Austin, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-08

    ... 78705, Telephone (512) 404- 1900. Thursday, April 7, 2011; 5 p.m. to 8 p.m.; at the George Washington Carver Museum (Museum Foyer), 1161 Angelina Street, Austin, TX 78702, Telephone (512) 974-4926....

  8. Tank characterization report for single-shell tank 241-TX-107

    SciTech Connect

    Sasaki, L.M., Westinghouse Hanford

    1996-07-10

    This document summarizes the information on the historical uses, present status, and the sampling and analysis results of waste stored in Tank 241-TX-107. This report supports the requirements of Tri-party Agreement Milestone M-44-09.

  9. TOMORROW: EPA Recognizing Gulf Guardian Award Winners in Corpus Christi, TX

    EPA Pesticide Factsheets

    DALLAS - (July 29, 2015) The U.S. Environmental Protection Agency's (EPA) Gulf of Mexico Program will hold the 13 th annual Gulf Guardians Awards ceremony in Corpus Christi, TX, this Thursday, July 30.

  10. 76 FR 26658 - Proposed Establishment of Class E Airspace; Ranger, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-09

    ... also be examined during normal business hours at the office of the Central Service Center, 2601 Meacham... Airspace areas extending upward from 700 feet or more above the surface of the earth. * * * * * ASW TX...

  11. 76 FR 1511 - Amendment of Class D Airspace; Fort Worth NAS JRB (Carswell Field), TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-11

    ... navigation aids, at the request of the U.S. Navy, that are listed in the description. This action does not...) Class D airspace, Fort Worth, TX, and the navigation aids, to coincide with the FAAs...

  12. 33 CFR 165.809 - Security Zones; Port of Port Lavaca-Point Comfort, Point Comfort, TX and Port of Corpus Christi...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Lavaca-Point Comfort, Point Comfort, TX and Port of Corpus Christi Inner Harbor, Corpus Christi, TX. 165... Lavaca-Point Comfort, Point Comfort, TX and Port of Corpus Christi Inner Harbor, Corpus Christi, TX. (a) Location. The following area is designated as a security zone: all waters of the Corpus Christi...

  13. ArgTX-636, a polyamine isolated from spider venom: A novel class of melanogenesis inhibitors.

    PubMed

    Verdoni, Marion; Roudaut, Hermine; De Pomyers, Harold; Gigmes, Didier; Bertin, Denis; Luis, José; Bengeloune, Abd Haq; Mabrouk, Kamel

    2016-11-15

    To discover new molecules with an inhibitory activity of melanogenesis a hundred of scorpions, snakes, spiders and amphibians venoms were screened for their capacity to inhibit mushroom tyrosinase using 3,4-l-dihydroxyphenylalanine (l-DOPA) as substrate. The Argiope lobata spider venom proved to be the most active. HPLC fraction containing Argiotoxine-636 (ArgTX-636), a polyamine known for its numerous biological activities, was found to also show a good regulation activity of melanogenesis by inhibiting DOPA and 5,6-dihydroxyindole-2-carboxylic acid (DHICA) oxidases activities, wore by tyrosinase (TYR) and tyrosinase-related protein 1 (TRP-1), respectively. Our results demonstrate that ArgTX-636 reduced the mushroom tyrosinase activity in a dose-dependent way with a maximal half inhibitory concentration (IC50) value of 8.34μM, when l-DOPA is used as substrate. The Lineweaver-Burk study showed that ArgTX-636 is a mixed type inhibitor of the diphenolase activity. Moreover, ArgTX-636 inhibits DHICA oxydase activity of mushroom tyrosinase activity with IC50 at 41.3μM. ArgTX-636 has no cytotoxicity in B16F10 melanoma cells at concentrations up to 42.1μM. The effect of ArgTX-636 on melanogenesis showed that melanin production in B16F10 melanoma cell decreased by approximatively 70% compared to untreated cells. ArgTX-636 displayed no significant effect on the TYR expression while the protein level of TRP-1 decreased in B16F10 cells. Thus, ArgTX-636 could have particular interest for cosmetic and/or pharmaceutical use in order to reduce important dermatoses in black and mixed skins.

  14. Synthesis and electrochemical performance of Ti3C2Tx with hydrothermal process

    NASA Astrophysics Data System (ADS)

    Wang, Libo; Zhang, Heng; Wang, Bo; Shen, Changjie; Zhang, Chuanxiang; Hu, Qianku; Zhou, Aiguo; Liu, Baozhong

    2016-09-01

    In this study, a simple hydrothermal method has been developed to prepare Ti3C2Tx from Ti3AlC2 as a high-performance electrode material for supercapacitors. This method is environmentally friendly and has a low level of danger. The morphology and structure of the Ti3C2Tx can be controlled by hydrothermal reaction time, temperature and NH4F amounts. The prepared Ti3C2Tx was characterized by X-ray diffraction, field emission scanning electron microscopy, Raman spectroscopy, X-ray photoelectron spectroscopy and Brunauer-Emmet-Teller. The results show that the prepared Ti3C2Tx is terminated by O, OH, and F groups. The electrochemical properties of the Ti3C2Tx sample exhibit specific capacitance up to 141 Fcm-3 in 3 M KOH aqueous electrolyte, and even after 1000 cycles, no significant degradation of the volumetric capacitance was observed. These results indicate that the Ti3C2Tx material prepared by this hydrothermal method can be used in high performance supercapacitors.

  15. Aerospace medicine at Brooks AFB, TX: hail and farewell.

    PubMed

    Nunneley, Sarah A; Webb, James T

    2011-05-01

    With the impending termination of USAF operations at Brooks Air Force Base (AFB) in San Antonio, TX, it is time to consider its historic role in Aerospace Medicine. The base was established in 1917 as a flight training center for the U.S. Army Air Service and in 1926 became home to its School of Aviation Medicine. The school moved to San Antonio's Randolph Field in 1931, but in 1959 it returned to Brooks where it occupied new facilities to support its role as a national center for U.S. Air Force aerospace medicine, including teaching, clinical medicine, and research. The mission was then expanded to encompass support of U.S. military and civilian space programs. With the abrupt termination of the military space program in 1969, research at Brooks focused on clinical aviation medicine and support of advanced military aircraft while continuing close cooperation with NASA in support of orbital spaceflight and the journey to the Moon. Reorganization in the 1990s assigned all research functions at Brooks to the Human Systems Division and its successors, leaving to USAFSAM the missions related to clinical work and teaching. In 2002 the USAF and the city of San Antonio implemented shared operation of Brooks as a "City-Base" in the hope of deflecting threatened closure. Nevertheless, under continuing pressure to consolidate military facilities in the United States, the 2005 Base Closure and Realignment Commission ordered Brooks closed by 2011, with its aerospace medicine functions relocated to new facilities at Wright-Patterson AFB in Dayton, OH.

  16. 77 FR 47816 - Foreign-Trade Zone 12-McAllen, TX Application for Subzone TST NA TRIM, LLC Hidalgo, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 12--McAllen, TX Application for Subzone TST NA TRIM, LLC... TST NA TRIM, LLC, located in Hidalgo, Texas. The application was submitted pursuant to the...

  17. Presynaptic Proteins as Markers of the Neurotoxic Activity of BmjeTX-I and BmjeTX-II Toxins from Bothrops marajoensis (Marajó Lancehead) Snake Venom

    PubMed Central

    Lisboa, Antonio; Melaré, Rodolfo; Franco, Junia R. B.; Bis, Carolina V.; Gracia, Marta; Ponce-Soto, Luis A.; Marangoni, Sérgio; Rodrigues-Simioni, Léa; da Cruz-Höfling, Maria Alice

    2016-01-01

    Neuromuscular preparations exposed to B. marajoensis venom show increases in the frequency of miniature end-plate potentials and twitch tension facilitation followed by presynaptic neuromuscular paralysis, without evidences of muscle damage. Considering that presynaptic toxins interfere into the machinery involved in neurotransmitter release (synaptophysin, synaptobrevin, and SNAP25 proteins), the main objective of this communication is to analyze, by immunofluorescence and western blotting, the expression of the synaptic proteins, synaptophysin, synaptobrevin, and SNAP25 and by myography, light, and transmission electron microscopy the pathology of motor nerve terminals and skeletal muscle fibres of chick biventer cervicis preparations (CBC) exposed in vitro to BmjeTX-I and BmjeTX-II toxins from B. marajoensis venom. CBC incubated with toxins showed irreversible twitch tension blockade and unaffected KCl- and ACh-evoked contractures, and the positive colabelling of acetylcholine receptors confirmed that their action was primarily at the motor nerve terminal. Hypercontraction and loose myofilaments and synaptic vesicle depletion and motor nerve damage indicated that the toxins displayed both myotoxic and neurotoxic effect. The blockade resulted from interference on synaptophysin, synaptobrevin, and SNAP25 proteins leading to the conclusion that BmjeTX-I and BmjeTX-II affected neurotransmitter release machinery by preventing the docking of synaptic vesicles to the axolemma of the nerve terminal. PMID:27635261

  18. Functional analysis of the alpha-neurotoxin, BmalphaTX14, derived from the Chinese scorpion, Buthus martensii Karsch.

    PubMed

    Wang, Kun; Yin, Shi-Jin; Lu, Meng; Yi, Hong; Dai, Chao; Xu, Xiu-Jing; Cao, Zhi-Jian; Wu, Ying-Liang; Li, Wen-Xin

    2006-11-01

    The gene encoding the BmalphaTX14 (alpha-neurotoxin TX14) protein, derived from the cDNA library of the Chinese scorpion Buthus martensii Karsch, was expressed in Pichia pastoris. The recombinant protein was purified by metal chelate affinity chromatography and gel filtration chromatography. Using patch-clamp technique, electrophysiological activity of rBmalphaTX14 was identified. In the neurons isolated from mice trigeminal root ganglion, the Na+ current amplitude was reduced by 80% under whole cell patch-clamp recording. There were no apparent modifications to the gating mechanism in the presence of rBmalphaTX14. Although BmalphaTX14 shared a high amino acid sequence similarity with other typical alpha-toxins, it has different effects on neurons. Further electrophysiological analysis suggested that rBmalphaTX14 selectively blocked Na+ channels and is a member of a new group of scorpion toxins.

  19. RCRA Assessment Plan for Single-Shell Tank Waste Management Area TX-TY

    SciTech Connect

    Horton, Duane G.

    2007-03-26

    WMA TX-TY contains underground, single-shell tanks that were used to store liquid waste that contained chemicals and radionuclides. Most of the liquid has been removed, and the remaining waste is regulated under the RCRA as modi¬fied in 40 CFR Part 265, Subpart F and Washington State’s Hazardous Waste Management Act . WMA TX-TY was placed in assessment monitoring in 1993 because of elevated specific conductance. A groundwater quality assessment plan was written in 1993 describing the monitoring activities to be used in deciding whether WMA TX-TY had affected groundwater. That plan was updated in 2001 for continued RCRA groundwater quality assessment as required by 40 CFR 265.93 (d)(7). This document further updates the assessment plan for WMA TX-TY by including (1) information obtained from ten new wells installed at the WMA after 1999 and (2) information from routine quarterly groundwater monitoring during the last five years. Also, this plan describes activities for continuing the groundwater assessment at WMA TX TY.

  20. Capacitance of Ti3C2Tx MXene in ionic liquid electrolyte

    NASA Astrophysics Data System (ADS)

    Lin, Zifeng; Barbara, Daffos; Taberna, Pierre-Louis; Van Aken, Katherine L.; Anasori, Babak; Gogotsi, Yury; Simon, Patrice

    2016-09-01

    Ti3C2Tx MXene, a two-dimensional (2D) early transition metal carbide, has shown an extremely high volumetric capacitance in aqueous electrolytes, but in a narrow voltage window (less than 1.23 V). The utilization of MXene materials in ionic liquid electrolytes with a large voltage window has never been addressed. Here, we report the preparation of the Ti3C2Tx MXene ionogel film by vacuum filtration for use as supercapacitor electrodes operating in 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (EMI-TFSI) neat ionic liquid electrolyte. Due to the disordered structure of the Ti3C2Tx hydrogel film and a stable spacing after vacuum drying, achieved through ionic liquid electrolyte immersion of the Ti3C2Tx hydrogel film, the Ti3C2Tx surface became accessible to EMI+ and TFSI- ions. A capacitance of 70 F g-1 together with a large voltage window of 3 V was obtained at a scan rate of 20 mV s-1 in neat EMI-TFSI electrolyte. The electrochemical signature indicates a capacitive behavior even at a high scan rate (500 mV s-1) and a high power performance. This work opens up the possibilities of using MXene materials with various ionic liquid electrolytes.

  1. Dosimetric characteristics of novalis Tx system with high definition multileaf collimator.

    PubMed

    Chang, Zheng; Wang, Zhiheng; Wu, Q Jackie; Yan, Hui; Bowsher, Jim; Zhang, Junan; Yin, Fang-Fang

    2008-10-01

    A new Novalis Tx system equipped with a high definition multileaf collimator (HDMLC) recently became available to perform both image-guided radiosurgery and conventional radiotherapy. It is capable of delivering a highly conformal radiation dose with three energy modes: 6 MV photon energy, 15 MV photon energy, and 6 MV photon energy in a stereotactic radiosurgery mode with 1000 MU/min dose rate. Dosimetric characteristics of the new Novalis Tx treatment unit with the HDMLC are systematically measured for commissioning. A high resolution diode detector and miniion-chamber detector are used to measure dosimetric data for a range of field sizes from 4 x 4 mm to 400 x 400 mm. The commissioned Novalis Tx system has passed the RPC stereotactic radiosurgery head phantom irradiation test. The Novalis Tx system not only expands its capabilities with three energy modes, but also achieves better beam conformity and sharer beam penumbra with HDMLC. Since there is little beam data information available for the new Novalis Tx system, we present in this work the dosimetric data of the new modality for reference and comparison.

  2. Capacitance of Ti3C2Tx MXene in Ionic Liquid Electrolyte

    DOE PAGES

    Lin, Zifeng; Barbara, Daffos; Taberna, Pierre-Louis; ...

    2016-04-14

    Ti3C2Tx MXene, a two-dimensional (2D) early transition metal carbide, has shown an extremely high volumetric capacitance in aqueous electrolytes, but in a narrow voltage window (less than 1.23 V). The utilization of MXene materials in ionic liquid electrolytes with a large voltage window has never been addressed. Here, we report the preparation of the Ti3C2Tx MXene ionogel film by vacuum filtration for use as supercapacitor electrodes operating in 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (EMI-TFSI) neat ionic liquid electrolyte. Due to the disordered structure of the Ti3C2Tx hydrogel film and a stable spacing after vacuum drying, achieved through ionic liquid electrolyte immersion of themore » Ti3C2Tx hydrogel film, the Ti3C2Tx surface became accessible to EMI+ and TFSI- ions. A capacitance of 70 F g-1 together with a large voltage window of 3 V was obtained at a scan rate of 20 mV s-1 in neat EMI-TFSI electrolyte. The electrochemical signature indicates a capacitive behavior even at a high scan rate (500 mV s-1) and a high power performance. This work opens up the possibilities of using MXene materials with various ionic liquid electrolytes.« less

  3. Effects of Receive-Only Inserts on SAR, B1+ Field and Tx Coil Performance

    PubMed Central

    Krishnamurthy, Narayanan; Zhao, Tiejun; Ibrahim, Tamer S

    2013-01-01

    Purpose To evaluate the effect of different cylindrical and close conforming receive only array designs on spin excitation and specific absorption rate (SAR) of a 7 Tesla transmit only head coil. Materials and Methods We developed FDTD models of different receive only array geometries. Cylindrical and close fitting helmet arrays with varying copper trace widths; a TEM Tx coil model and two head models were used in numerical simulations. Tx coil coupling was experimentally measured and validated with FDTD modeling. Results Changing copper trace width of loops in array models caused subtle changes in RF absorption (<5%). Changes in SAR distribution were observed in the head models with Rx-only inserts. Peak SAR increased (−1 to +15%) in different tissues for a mean B1+ in the brain of 2 μT. Total absorption in the head models for 1 Watt forward power increased (5 to 21%) in the heads with Rx-only inserts. Changes in RF absorption with different Rx-inserts indicate a change in RF radiation of the Tx coil even when changes in B1+ and coupling between ports of Tx coil were minimal. Conclusion Changes in local/global SAR and subtle changes in B1+ field distributions were observed with the presence of Rx-only inserts. Thus, incorporation of the receive-only array effects are needed when evaluating SAR and designing RF transmit pulse waveform parameters for shimming and/or Tx-SENSE for 7 T MRI. PMID:23913474

  4. LyeTx I, a potent antimicrobial peptide from the venom of the spider Lycosa erythrognatha.

    PubMed

    Santos, D M; Verly, R M; Piló-Veloso, D; de Maria, M; de Carvalho, M A R; Cisalpino, P S; Soares, B M; Diniz, C G; Farias, L M; Moreira, D F F; Frézard, F; Bemquerer, M P; Pimenta, A M C; de Lima, M E

    2010-06-01

    LyeTx I, an antimicrobial peptide isolated from the venom of Lycosa erythrognatha, known as wolf spider, has been synthesised and its structural profile studied by using the CD and NMR techniques. LyeTx I has shown to be active against bacteria (Escherichia coli and Staphylococcus aureus) and fungi (Candida krusei and Cryptococcus neoformans) and able to alter the permeabilisation of L: -alpha-phosphatidylcholine-liposomes (POPC) in a dose-dependent manner. In POPC containing cholesterol or ergosterol, permeabilisation has either decreased about five times or remained unchanged, respectively. These results, along with the observed low haemolytic activity, indicated that antimicrobial membranes, rather than vertebrate membranes seem to be the preferential targets. However, the complexity of biological membranes compared to liposomes must be taken in account. Besides, other membrane components, such as proteins and even specific lipids, cannot be discarded to be important to the preferential action of the LyeTx I to the tested microorganisms. The secondary structure of LyeTx I shows a small random-coil region at the N-terminus followed by an alpha-helix that reached the amidated C-terminus, which might favour the peptide-membrane interaction. The high activity against bacteria together with the moderate activity against fungi and the low haemolytic activity have indicated LyeTx I as a good prototype for developing new antibiotic peptides.

  5. Mobilization of Bacillus thuringiensis plasmid pTX14-3.

    PubMed

    Andrup, L; Bendixen, H H; Jensen, G B

    1995-05-01

    The Bacillus thuringiensis subsp. israelensis (Bti) plasmid pTX14-3 has been reported to contain a gene, mob14-3, with considerable homology to genes encoding mobilization proteins from other gram-positive bacteria. We have used the aggregation-mediated conjugation system recently discovered in Bti to compare the mobilization kinetics of different derivatives of plasmid pTX14-3. Plasmid pTX14-3 has been found to replicate by the rolling-circle mechanism and to contain a locus suppressing the formation of high-molecular-weight DNA. We found that deleting a DNA fragment containing this locus increased the transfer frequency about twofold. The mobilization frequency of the plasmid containing the intact mob14-3 gene did not indicate a mobilization-enhancing activity of the encoded polypeptide. However, the presence of the mob14-3 gene seemed to increase the stability of the plasmid in exponential growth.

  6. Cardiorespiratory Effect of Brevetoxin (PbTx-2) in Conscious, Tethered Rats

    DTIC Science & Technology

    1988-11-07

    calculation of median-effective dose (ED5 0 or LD5 0 ) and instructions in their use. Biometrics 8, 249. FIGURE LEGENDS Figure 1. Dose - response curve showing...8217). Figure 2. Dose - response curve showing effect of 1 hr infusion of PbTx-2 on core temperature in rats. (solid = 100 ug/kg; dashed = 50 ug/kg; dotted... Dose - response curve showing effect of 1 hr infusion of PbTx-2 on peripheral temperature in rats. [solid = 100 ug/kg; dashed = 50 ug/kg; dotted = 25

  7. Tank 241-TX-118 vapor sampling and analysis tank characterization report

    SciTech Connect

    Huckaby, J.L.

    1995-05-31

    Tank 241-TX-118 headspace gas and vapor samples were collected and analyzed to help determine the potential risks to tank farm workers due to fugitive emissions from the tank. The drivers and objectives of waste tank headspace sampling and analysis are discussed in {open_quotes}Program Plan for the Resolution of Tank Vapor Issues.{close_quotes} Tank 241-TX-118 was vapor sampled in accordance with {open_quotes}Data Quality Objectives for Generic In-Tank Health and Safety Issue Resolution.{close_quotes}

  8. Tank 241-TX-105 vapor sampling and analysis tank characterization report

    SciTech Connect

    Huckaby, J.L.

    1995-05-31

    Tank 241-TX-105 headspace gas and vapor samples were collected and analyzed to help determine the potential risks to tank farm workers due to fugitive emissions from the tank. The drivers and objectives of waste tank headspace sampling and analysis are discussed in {open_quotes}Program Plan for the Resolution of Tank Vapor Issues.{close_quotes} Tank 241-TX-105 was vapor sampled in accordance with {open_quotes}Data Quality Objectives for Generic In-Tank Health and Safety Issue Resolution.{close_quotes}

  9. 78 FR 4124 - Foreign-Trade Zone 68-El Paso, TX, Application for Subzone, Expeditors International of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-18

    ... Foreign-Trade Zones Board Foreign-Trade Zone 68--El Paso, TX, Application for Subzone, Expeditors International of Washington, Inc., El Paso, TX An application has been submitted to the Foreign-Trade Zones Board (the Board) by the City of El Paso, grantee of FTZ 68, requesting special-purpose subzone...

  10. 76 FR 6613 - AP Gas & Electric (TX), LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-07

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission AP Gas & Electric (TX), LLC; Supplemental Notice That Initial Market-Based... supplemental notice in the above-referenced proceeding of AP Gas & Electric (TX), LLC's application for...

  11. 78 FR 9907 - TGP Development Company, LLC; TGP Flying Cloud Holdings, LLC; WEC TX Company, LLC v. Arizona...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission TGP Development Company, LLC; TGP Flying Cloud Holdings, LLC; WEC TX Company... Development Company, LLC, TGP Flying Cloud Holdings, LLC, and WEC TX Company, LLC (collectively,...

  12. [Genomic DNA sequences and functional expression, purification of BmalphaTX14 neurotoxin from scorpion Buthus martensii Karsch].

    PubMed

    Lü, Meng; Wang, Kun; Cao, Zhi-Jian; Jiang, Da-He; Mao, Xin; Li, Wen-Xin

    2005-11-01

    Based on the full-length cDNA of BmalphaTX14 from Chinese scorpion Buthus martensii Karsch (BmK), gene of the mature peptide of BmalphaTX14 was cloned into the yeast expression vector pPIC9K. After transforming, screening and inducing, tricine-SDS-PAGE and Western blot proved that rBmalphaTX14 protein was expressed in the medium for up to 84 hours, getting nearly 120 mg/L. Recombinant BmalphaTX14 was purified rapidly and efficiently through Ni-NTA-agarose, polyethylene glycol precipitation and gel filtration chromatography. The purified rBmalphaTX14 proved to have the anti-insect activity by toxicity assay. Meanwhile, genomic gene of BmalphaTX14 was cloned and sequenced by PCR method, sequence analysis of this gene showed that BmalphaTX14 had an intron of 408 base pairs located at the signal peptide encoding region, which was similar with the characteristic of other alpha-type sodium ion-channel toxin. Considering both the genomic organization and the peptide function, BmaTX14 proved to be a membership belonging to alpha-type sodium ion-channel toxin.

  13. 75 FR 65232 - Security Zones; Sabine Bank Channel, Sabine Pass Channel and Sabine-Neches Waterway, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-22

    ... measures necessary. In addition, it establishes security zones encompassing the mooring basins of LNG carriers while they are moored at the Golden Pass LNG facility in Sabine, TX and/or the Sabine Pass LNG... ] mooring basins at the Golden Pass LNG facility in Sabine, TX and the Sabine Pass LNG facility located...

  14. 76 FR 1519 - Security Zones; Sabine Bank Channel, Sabine Pass Channel and Sabine-Neches Waterway, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-11

    ... encompassing the mooring basins of LNG carriers while they are moored at the Golden Pass LNG facility in Sabine, TX and/or the Sabine Pass LNG facility located in Cameron Parish, LA. DATES: This rule is effective... mooring basins at the Golden Pass LNG facility in Sabine, TX and the Sabine Pass LNG facility located...

  15. rBmαTX14 Increases the Life Span and Promotes the Locomotion of Caenorhabditis Elegans

    PubMed Central

    Xu, Jie; Wan, Lu; Teng, Kaixuan; Xiang, Jin; Zhang, Rui; Huang, Zebo; Liu, Yongmei; Li, Wenhua; Liu, Xin

    2016-01-01

    The scorpion has been extensively used in various pharmacological profiles or as food supplies. The exploration of scorpion venom has been reported due to the presence of recombinant peptides. rBmαTX14 is an α-neurotoxin extracted from the venom gland of the East Asian scorpion Buthus martensii Karsch and can affect ion channel conductance. Here, we investigated the functions of rBmαTX14 using the Caenorhabditis elegans model. Using western blot analysis, rBmαTX14 was shown to be expressed both in the cytoplasm and inclusion bodies in the E.coli Rosetta (DE3) strain. Circular dichroism spectroscopy analysis demonstrated that purified rBmαTX14 retained its biological structures. Next, feeding nematodes with E.coli Rosetta (DE3) expressing rBmαTX14 caused extension of the life span and promoted the locomotion of the nematodes. In addition, we identified several genes that play various roles in the life span and locomotion of C. elegans through microarray analysis and quantitative real-time PCR. Furthermore, if the amino acid site H15 of rBmαTX14 was mutated, rBmαTX14 no longer promoted the C. elegans life span. In conclusion, the results not only demonstrated the functions and mechanism of rBmαTX14 in C. elegans, but also provided the new sight in the utility of recombinant peptides from scorpion venom. PMID:27611314

  16. 33 CFR 165.819 - Security Zone; Sabine Bank Channel, Sabine Pass Channel and Sabine-Neches Waterway, TX.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Sabine-Neches Waterway, TX. (a) Location. (1) The following LNG facility mooring basins are designated as fixed security zones whenever LNG carriers are moored within them: (i) Golden Pass LNG, Sabine TX: All...°45′50″ N, 093°55′17″ W. (ii) Sabine Pass LNG, Cameron Parish, LA: All mooring basin waters north of...

  17. 33 CFR 165.819 - Security Zone; Sabine Bank Channel, Sabine Pass Channel and Sabine-Neches Waterway, TX.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Sabine-Neches Waterway, TX. (a) Location. (1) The following LNG facility mooring basins are designated as fixed security zones whenever LNG carriers are moored within them: (i) Golden Pass LNG, Sabine TX: All...°45′50″ N, 093°55′17″ W. (ii) Sabine Pass LNG, Cameron Parish, LA: All mooring basin waters north of...

  18. 33 CFR 165.819 - Security Zone; Sabine Bank Channel, Sabine Pass Channel and Sabine-Neches Waterway, TX.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Sabine-Neches Waterway, TX. (a) Location. (1) The following LNG facility mooring basins are designated as fixed security zones whenever LNG carriers are moored within them: (i) Golden Pass LNG, Sabine TX: All...°45′50″ N, 093°55′17″ W. (ii) Sabine Pass LNG, Cameron Parish, LA: All mooring basin waters north of...

  19. Chemical synthesis and 1H-NMR 3D structure determination of AgTx2-MTX chimera, a new potential blocker for Kv1.2 channel, derived from MTX and AgTx2 scorpion toxins

    PubMed Central

    Pimentel, Cyril; M'Barek, Sarrah; Visan, Violetta; Grissmer, Stephan; Sampieri, François; Sabatier, Jean-Marc; Darbon, Hervé; Fajloun, Ziad

    2008-01-01

    Agitoxin 2 (AgTx2) is a 38-residue scorpion toxin, cross-linked by three disulfide bridges, which acts on voltage-gated K+ (Kv) channels. Maurotoxin (MTX) is a 34-residue scorpion toxin with an uncommon four-disulfide bridge reticulation, acting on both Ca2+-activated and Kv channels. A 39-mer chimeric peptide, named AgTx2-MTX, was designed from the sequence of the two toxins and chemically synthesized. It encompasses residues 1–5 of AgTx2, followed by the complete sequence of MTX. As established by enzyme cleavage, the new AgTx2-MTX molecule displays half-cystine pairings of the type C1–C5, C2–C6, C3–C7, and C4–C8, which is different from that of MTX. The 3D structure of AgTx2-MTX solved by 1H-NMR, revealed both α-helical and β-sheet structures, consistent with a common α/β scaffold of scorpion toxins. Pharmacological assays of AgTx2-MTX revealed that this new molecule is more potent than both original toxins in blocking rat Kv1.2 channel. Docking simulations, performed with the 3D structure of AgTx2-MTX, confirmed this result and demonstrated the participation of the N-terminal domain of AgTx2 in its increased affinity for Kv1.2 through additional molecular contacts. Altogether, the data indicated that replacement of the N-terminal domain of MTX by the one of AgTx2 in the AgTx2-MTX chimera results in a reorganization of the disulfide bridge arrangement and an increase of affinity to the Kv1.2 channel. PMID:18042681

  20. Chemical synthesis and 1H-NMR 3D structure determination of AgTx2-MTX chimera, a new potential blocker for Kv1.2 channel, derived from MTX and AgTx2 scorpion toxins.

    PubMed

    Pimentel, Cyril; M'Barek, Sarrah; Visan, Violetta; Grissmer, Stephan; Sampieri, François; Sabatier, Jean-Marc; Darbon, Hervé; Fajloun, Ziad

    2008-01-01

    Agitoxin 2 (AgTx2) is a 38-residue scorpion toxin, cross-linked by three disulfide bridges, which acts on voltage-gated K(+) (Kv) channels. Maurotoxin (MTX) is a 34-residue scorpion toxin with an uncommon four-disulfide bridge reticulation, acting on both Ca(2+)-activated and Kv channels. A 39-mer chimeric peptide, named AgTx2-MTX, was designed from the sequence of the two toxins and chemically synthesized. It encompasses residues 1-5 of AgTx2, followed by the complete sequence of MTX. As established by enzyme cleavage, the new AgTx2-MTX molecule displays half-cystine pairings of the type C1-C5, C2-C6, C3-C7, and C4-C8, which is different from that of MTX. The 3D structure of AgTx2-MTX solved by (1)H-NMR, revealed both alpha-helical and beta-sheet structures, consistent with a common alpha/beta scaffold of scorpion toxins. Pharmacological assays of AgTx2-MTX revealed that this new molecule is more potent than both original toxins in blocking rat Kv1.2 channel. Docking simulations, performed with the 3D structure of AgTx2-MTX, confirmed this result and demonstrated the participation of the N-terminal domain of AgTx2 in its increased affinity for Kv1.2 through additional molecular contacts. Altogether, the data indicated that replacement of the N-terminal domain of MTX by the one of AgTx2 in the AgTx2-MTX chimera results in a reorganization of the disulfide bridge arrangement and an increase of affinity to the Kv1.2 channel.

  1. 77 FR 66743 - Radio Broadcasting Services; Crowell, Knox City, Quanah, and Rule, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-07

    ... COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Crowell, Knox City, Quanah, and Rule, TX AGENCY... of Allotments by substituting Channel 293A for vacant Channel 291A at Knox City, Texas; Channel 288C2.... Channel 293A can be allotted to Knox City, Texas at reference coordinates 33-25-55 NL and 99-47-43 WL,...

  2. 77 FR 60103 - Approval of Subzone Status; TST NA TRIM, LLC; Hidalgo, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-02

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Approval of Subzone Status; TST NA TRIM, LLC; Hidalgo, TX On August 3, 2012, the... limit of FTZ 12, on behalf of TST NA TRIM, LLC, in Hidalgo, Texas. The application was processed...

  3. Encounters with Wisdom: A Case Study of Community Worker Reflection Circles in San Antonio, TX

    ERIC Educational Resources Information Center

    Arispe, Don D.

    2013-01-01

    This case study focuses upon the transformational experiences of 28 social service and pastoral workers engaged in a Reflection Circle Process (RCP) in San Antonio, TX. The RCP involves the writing of a holistic journal entry, known as a process note, coupled with an in-depth exploration of the note with the help of a group of trusted peers, known…

  4. 75 FR 8994 - Certification of the Attorney General; Williamson County, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-26

    ... Certification of the Attorney General; Williamson County, TX In accordance with Section 8 of the Voting Rights... of the Attorney General and the Director of the Census made under Section 4(b) of the Voting Rights...). Dated: February 23, 2010. Eric H. Holder Jr., Attorney General of the United States. BILLING CODE...

  5. 76 FR 15857 - Radio Broadcasting Services; Early and Lake Brownwood, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ... COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Early and Lake Brownwood, TX AGENCY: Federal... the Congressional Review Act, see U.S.C. 801(a)(1)(A). List Of Subjects in 47 CFR Part 73 Radio, Radio... CFR part 73 as follows: PART 73--RADIO BROADCAST SERVICES 0 1. The authority citation for Part...

  6. 78 FR 13857 - Foreign-Trade Zone 84-Houston, TX; Notification of Proposed Production Activity; Toshiba...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-01

    ... Foreign-Trade Zones Board Foreign-Trade Zone 84--Houston, TX; Notification of Proposed Production Activity; Toshiba International Corporation (Hybrid Electric Vehicle Motors and Generators Production) The Port of Houston Authority, grantee of FTZ 84, submitted a notification of proposed production activity on...

  7. 76 FR 73587 - Foreign-Trade Zone 183-Austin, Tx; Site Renumbering Notice

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-29

    ... Foreign-Trade Zones Board Foreign-Trade Zone 183--Austin, Tx; Site Renumbering Notice Foreign-Trade Zone... currently consists of 8 ``sites'' totaling some 2,818 acres in the Austin area. The current updates does not... follows: Site 1 (33 acres)--Interchange w/n the Austin Enterprise Zone, located at Bolm Road and...

  8. Streptomyces venezuelae TX-TL - a next generation cell-free synthetic biology tool.

    PubMed

    Moore, Simon J; Lai, Hung-En; Needham, Hannah; Polizzi, Karen M; Freemont, Paul S

    2017-01-31

    Streptomyces venezuelae is a promising chassis in synthetic biology for fine chemical and secondary metabolite pathway engineering. The potential of S. venezuelae could be further realized by expanding its capability with the introduction of its own in vitro transcription-translation (TX-TL) system. TX-TL is a fast and expanding technology for bottom-up design of complex gene expression tools, biosensors and protein manufacturing. Herein, we introduce a S. venezuelae TX-TL platform by reporting a streamlined protocol for cell-extract preparation, demonstrating high-yield synthesis of a codon-optimized sfGFP reporter and the prototyping of a synthetic tetracycline-inducible promoter in S. venezuelae TX-TL based on the tetO-TetR repressor system. The aim of this system is to provide a host for the homologous production of exotic enzymes from Actinobacteria secondary metabolism in vitro. As an example, the authors demonstrate the soluble synthesis of a selection of enzymes (12-70 kDa) from the Streptomyces rimosus oxytetracycline pathway.

  9. 78 FR 31839 - Establishment of Class E Airspace; Beeville-Chase Field, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-28

    ... Federal Aviation Administration 14 CFR Part 71 Establishment of Class E Airspace; Beeville-Chase Field, TX... Register of March 28, 2013. The title and airspace designation are corrected to read Beeville-Chase Field... Register document FAA 2012-0821, Airspace Docket No. 12- ASW-8, establishes Class E Airspace at Chase...

  10. Preliminary tank characterization report for single-shell tank 241-TX-115: Best-Basis inventory

    SciTech Connect

    Kupfer, M.J.

    1997-09-02

    An effort is underway to provide waste inventory estimates that will serve as standard characterization source terms for the various waste management activities. As part of this effort, an evaluation of available information for single-shell tank 241-TX-115 was performed, and a best-basis inventory was established. This work follows the methodology that was established by the standard inventory task.

  11. Preliminary tank characterization report for single-shell tank 241-TX-116: best-basis inventory

    SciTech Connect

    Place, D.E.

    1997-06-01

    An effort is underway to provide waste inventory estimates that will serve as standard characterization source terms for the various waste management activities. As part of this effort, an evaluation of available information for single-shell tank 241-TX-116 was performed, and a bost-basis inventory was established. This work follows the methodology that was established by the standard inventory task.

  12. Preliminary tank characterization report for single-shell tank 241-TX-104: best-basis inventory

    SciTech Connect

    Kupfer, M.J.

    1997-09-02

    An effort is underway to provide waste inventory estimates that will serve as standard characterization source terms for the various waste management activities. As part of this effort, an evaluation of available information for single-shell tank 241-TX-104 was performed, and a best-basis inventory was established. This work follows the methodology that was established by the standard inventory task.

  13. Preliminary tank characterization report for single-shell tank 241-TX-105: best-basis inventory

    SciTech Connect

    Kupfer, M.J.

    1997-08-01

    An effort is underway to provide waste inventory estimates that will serve as standard characterization source terms for the various waste management activities. As part of this effort, an evaluation of available information for single-shell tank 241-TX-105 was performed, and a best-basis inventory was established. This work follows the methodology that was established by the standard inventory task.

  14. Preliminary tank characterization report for single-shell tank 241-TX-117: best-basis inventory

    SciTech Connect

    Place, D.E.

    1997-06-01

    An effort is underway to provide waste inventory estimates that will serve as standard characterization source terms for the various waste management activities. As part of this effort, an evaluation of available information for single-shell tank 241-TX-117 was performed, and a best-basis inventory was established. This work follows the methodology that was established by the standard inventory task.

  15. Preliminary tank characterization report for single-shell tank 241-TX-110: Best-basis inventory

    SciTech Connect

    Place, D.E.

    1997-08-26

    An effort is underway to provide waste inventory estimates that will serve as standard characterization source terms for the various waste management activities. As part of this effort, an evaluation of available information for single-shell tank 241-TX-110 was performed, and a best-basis inventory was established. This work follows the methodology that was established by the standard inventory task.

  16. Preliminary tank characterization report for single-shell tank 241-TX-109: best-basis inventory

    SciTech Connect

    Place, D.E.

    1997-08-26

    An effort is underway to provide waste inventory estimates that will serve as standard characterization source terms for the various waste management activities. As part of this effort, an evaluation of available information for single-shell tank 241-TX-109 was performed, and a best-basis inventory was established. This work follows the methodology that was established by the standard inventory task.

  17. Preliminary tank characterization report for single-shell tank 241-TX-102: best-basis inventory

    SciTech Connect

    Kupfer, M.J.

    1997-09-02

    An effort is underway to provide waste inventory estimates that will serve as standard characterization source terms for the various waste management activities. As part of this effort, an evaluation of available information for single-shell tank 241-TX-102 was performed, and a best-basis inventory was established. This work follows the methodology that was established by the standard inventory task.

  18. 75 FR 66301 - Amendment of Class E Airspace; Corpus Christi, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-28

    ....) Ingleside, T.P. McCampbell Airport, TX (Lat. 27 54'47'' N., long. 97 12'41'' W.) Robstown, Nueces County....3-mile radius of Nueces County Airport, and within a 7.6-mile radius of Aransas County Airport,...

  19. 75 FR 31677 - Amendment of Class E Airspace; Corpus Christi, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-04

    ....) Ingleside, T.P. McCampbell Airport, TX (Lat. 27 54'47'' N., long. 97 12'41'' W.) Robstown, Nueces County... Airport, and within a 6.3-mile radius of Nueces County Airport, and within a 7.6-mile radius of...

  20. 78 FR 14909 - Amendment of Class B Airspace Description; Houston, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-08

    ... Flexibility Act. The FAA's authority to issue rules regarding aviation safety is found in Title 49 of the... Federal Aviation Administration 14 CFR Part 71 RIN 2120-AA66 Amendment of Class B Airspace Description; Houston, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule, technical...

  1. 77 FR 65361 - Reorganization/Expansion of Foreign-z Under Alternative Site Framework; Galveston, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-26

    ...; Galveston, TX Pursuant to its authority under the Foreign-Trade Zones Act of June 18, 1934, as amended (19 U... or reorganization of zones; Whereas, the Board of Trustees of the Galveston Wharves, grantee of... authority to reorganize under the ASF with a service area of Galveston County, Texas, within and adjacent...

  2. 78 FR 70900 - Proposed Modification of Area Navigation (RNAV) Route Q-20, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-27

    ... (RNAV) Route Q-20, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of proposed rulemaking (NPRM). SUMMARY: This action proposes to modify RNAV route Q-20 in support of the Houston Optimization of Airspace and Procedures in a Metroplex (OAPM) project. This proposed action re-aligns Q-20...

  3. 75 FR 12726 - Expansion/Reorganization of Foreign-Trade Zone 149; Port Freeport, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-17

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Expansion/Reorganization of Foreign-Trade Zone 149; Port Freeport, TX Pursuant to its authority under the Foreign-Trade Zones Act of June 18, 1934, as amended (19 U.S.C....

  4. 78 FR 26799 - Waterway Suitability Assessment for Expansion of Liquefied Gas Terminals; Beaumont, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-08

    ... SECURITY Coast Guard Waterway Suitability Assessment for Expansion of Liquefied Gas Terminals; Beaumont, TX... construction to expand or modify marine terminal operations in an existing facility handling Liquefied Natural... LHG marine traffic in the associated waterway. Martin Operating Partnership's Stanolind Cut...

  5. EPA Announces 2015 ENERGY STAR Certified Manufacturing Plants, Two Midlothian, TX, facilities among those recognized

    EPA Pesticide Factsheets

    DALLAS - (Feb. 24, 2016) The U.S. Environmental Protection Agency (EPA) announced today that two facilities in Midlothian, TX-the Holcim and Martin Marietta Materials cement facilities-are among the 70 manufacturing plants across the nation that ach

  6. 78 FR 55214 - Annual Marine Events in the Eighth Coast Guard District, Sabine River; Orange, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 100 Annual Marine Events in the Eighth Coast Guard District, Sabine River; Orange, TX AGENCY: Coast Guard, DHS. ACTION: Notice of enforcement of regulation. SUMMARY: The...

  7. 77 FR 2448 - Special Local Regulation; HITS Triathlon; Corpus Christi Bayfront, Corpus Christi, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-18

    ... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulation; HITS Triathlon; Corpus Christi Bayfront, Corpus Christi, TX AGENCY: Coast Guard, DHS. ACTION: Temporary Final rule. SUMMARY: The Coast Guard is establishing a temporary Special Local Regulation in the Corpus Christi Bayfront area...

  8. 75 FR 31463 - Comal County Regional Habitat Conservation Plan, Comal County, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-03

    ... Fish and Wildlife Service Comal County Regional Habitat Conservation Plan, Comal County, TX AGENCY... statement, draft habitat conservation plan, and permit application; announcement of a public hearing... County has completed a draft Habitat Conservation Plan (dHCP) as part of the application package. We...

  9. 75 FR 52065 - SharePlus Federal Bank, Plano, TX; Approval of Conversion Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-24

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE TREASURY Office of Thrift Supervision SharePlus Federal Bank, Plano, TX; Approval of Conversion Application Notice is hereby given that on August 12, 2010, the Office of Thrift Supervision approved the application...

  10. 77 FR 18853 - Trinity River National Wildlife Refuge, Liberty County, TX; Comprehensive Conservation Plan and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-28

    ... Fish and Wildlife Service Trinity River National Wildlife Refuge, Liberty County, TX; Comprehensive Conservation Plan and Environmental Assessment AGENCY: Fish and Wildlife Service, Interior. ACTION: Notice of availability; request for comments. SUMMARY: We, the U.S. Fish and Wildlife Service (Service), announce...

  11. 33 CFR 165.827 - Regulated Navigation Area; Galveston Channel, TX.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Regulated Navigation Area; Galveston Channel, TX. 165.827 Section 165.827 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY REGULATED NAVIGATION AREAS AND LIMITED...

  12. 33 CFR 165.827 - Regulated Navigation Area; Galveston Channel, TX.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Regulated Navigation Area; Galveston Channel, TX. 165.827 Section 165.827 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY REGULATED NAVIGATION AREAS AND LIMITED...

  13. 75 FR 13433 - Safety Zone; Invista Inc Facility Docks, Victoria Barge Canal, Victoria, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-22

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Invista Inc Facility Docks, Victoria Barge Canal, Victoria, TX AGENCY: Coast Guard, DHS. ACTION: Interim final rule with request for comments. SUMMARY: The Coast Guard is establishing a safety zone for a partial blockage of the Victoria Barge...

  14. 76 FR 5196 - Aransas National Wildlife Refuge Complex, Aransas, Calhoun, and Refugio Counties, TX; Final...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-28

    ... Counties, TX; Final Comprehensive Conservation Plan and Finding of No Significant Impact for Environmental... plan (CCP) and finding of no significant impact (FONSI) for the environmental assessment (EA) for the... business hours at 500 Gold Avenue SW., Albuquerque, NM 87102. FOR FURTHER INFORMATION CONTACT: Dan...

  15. 46 CFR 7.105 - Marquesas Keys, FL to Rio Grande, TX.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 1 2012-10-01 2012-10-01 false Marquesas Keys, FL to Rio Grande, TX. 7.105 Section 7.105 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY PROCEDURES APPLICABLE TO THE PUBLIC BOUNDARY... the seaward limits of the territorial sea (as defined in 33 CFR 2.22(a)(1)) to Rio Grande, Texas...

  16. 46 CFR 7.105 - Marquesas Keys, FL to Rio Grande, TX.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 1 2011-10-01 2011-10-01 false Marquesas Keys, FL to Rio Grande, TX. 7.105 Section 7.105 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY PROCEDURES APPLICABLE TO THE PUBLIC BOUNDARY... the seaward limits of the territorial sea (as defined in 33 CFR 2.22(a)(1)) to Rio Grande, Texas...

  17. 46 CFR 7.105 - Marquesas Keys, FL to Rio Grande, TX.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Marquesas Keys, FL to Rio Grande, TX. 7.105 Section 7.105 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY PROCEDURES APPLICABLE TO THE PUBLIC BOUNDARY... the seaward limits of the territorial sea (as defined in 33 CFR 2.22(a)(1)) to Rio Grande, Texas...

  18. 75 FR 66013 - Proposed Amendment of Class E Airspace; Horseshoe Bay, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-27

    ... Resort Airport. Controlled airspace is necessary for the safety and management of IFR operations at the... Bay Resort non-directional beacon (NDB) at Horseshoe Bay Resort Airport, Horseshoe Bay, TX, has made this action necessary for the safety and management of Instrument Flight Rules (IFR) operations at...

  19. 75 FR 45557 - Prevailing Rate Systems; Definition of Tulsa County, OK, and Angelina County, TX, to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-03

    ...; ] OFFICE OF PERSONNEL MANAGEMENT 5 CFR Part 532 RIN 3206-AM22 Prevailing Rate Systems; Definition of Tulsa County, OK, and Angelina County, TX, to Nonappropriated Fund Federal Wage System Wage Areas AGENCY... area of application to the Oklahoma, OK, nonappropriated fund (NAF) Federal Wage System (FWS) wage...

  20. 78 FR 34121 - Notice of Inventory Completion: Dallas Water Utilities, Dallas, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-06

    ... National Park Service Notice of Inventory Completion: Dallas Water Utilities, Dallas, TX AGENCY: National Park Service, Interior. ] ACTION: Notice. SUMMARY: The Dallas Water Utilities has completed an... funerary objects should submit a written request to Dallas Water Utilities. If no additional...

  1. The solution structure of BmTx3B, a member of the scorpion toxin subfamily alpha-KTx 16.

    PubMed

    Wang, Yuefeng; Chen, Xiang; Zhang, Naixia; Wu, Gong; Wu, Houming

    2005-02-01

    This article reports the solution structure of BmTx3B (alpha-KTx16.2), a potassium channel blocker belonging to the subfamily alpha-KTx16, purified from the venom of the Chinese scorpion Buthus martensi Karsch. In solution, BmTx3B assumes a typical CSalphabeta motif, with an alpha-helix connected to a triple-stranded beta-sheet by 3 disulfide bridges, which belongs to the first structural group of short-chain scorpion toxins. On the other hand, BmTx3B is quite different from other toxins (such as ChTx and AgTx2) of this group in terms of the electrostatic and hydrophobic surface distribution. The functional surface (beta-face) of the molecule is characterized by less basic residues (only 2: Lys28 and Arg35) and extra aromatic residues (Phe1, Phe9, Trp15, and Tyr37). The peptide shows a great preference for the Kca1.1 channel over the Kv channel (about a 10(3)-fold difference). The model of BmTx3B/Kca1.1 channel complex generated by docking and dynamic simulation reveals that the stable binding between the BmTx3B and Kca1.1 channel is favored by a number of aromatic pi-pi stacking interactions. The influences of these structural features on the kinetic behavior of the toxin binding to Kca1.1 channel are also discussed.

  2. Binding sites and actions of Tx1, a neurotoxin from the venom of the spider Phoneutria nigriventer, in guinea pig ileum.

    PubMed

    Santos, R G; Diniz, C R; Cordeiro, M N; De Lima, M E

    1999-12-01

    Tx1, a neurotoxin isolated from the venom of the South American spider Phoneutria nigriventer, produces tail elevation, behavioral excitation and spastic paralysis of the hind limbs after intracerebroventricular injection in mice. Since Tx1 contracts isolated guinea pig ileum, we have investigated the effect of this toxin on acetylcholine release, as well as its binding to myenteric plexus-longitudinal muscle membranes from the guinea pig ileum. [125I]-Tx1 binds specifically and with high affinity (Kd = 0.36 +/- 0.02 nM) to a single, non-interacting (nH = 1.1), low capacity (Bmax 1.1 pmol/mg protein) binding site. In competition experiments using several compounds (including ion channel ligands), only PhTx2 and PhTx3 competed with [125I]-Tx1 for specific binding sites (K0.5 apparent = 7.50 x 10(-4) g/l and 1.85 x 10(-5) g/l, respectively). PhTx2 and PhTx3, fractions from P. nigriventer venom, contain toxins acting on sodium and calcium channels, respectively. However, the neurotoxin PhTx2-6, one of the isoforms found in the PhTx2 pool, did not affect [125I]-Tx1 binding. Tx1 reduced the [3H]-ACh release evoked by the PhTx2 pool by 33%, but did not affect basal or KCl-induced [3H]-ACh release. Based on these results, as well as on the homology of Tx1 with toxins acting on calcium channels (omega-Aga IA and IB) and its competition with [125I]-omega-Cono GVIA in the central nervous system, we suggest that the target site for Tx1 may be calcium channels.

  3. Cost-effectiveness analysis of TxDOT CNG fleet conversion, volume 2

    NASA Astrophysics Data System (ADS)

    Euritt, M. A.; Taylor, D. B.; Mahmassani, H.

    1992-08-01

    Increased emphasis on energy efficiency and air quality has resulted in a number of state and federal initiatives examining the use of alternative fuels for motor vehicles. Texas' program for alternate fuels includes compressed natural gas (CNG). Based on an analysis of 30-year life-cycle costs, development of a natural gas vehicle (NGV) program for the Texas Department of Transportation (TxDOT) would cost about $47 million (in 1991 dollars). These costs include savings from lower priced natural gas, infrastructure costs for a fast-fueling station, vehicle costs, and operating costs. The 30-year life-cycle costs translate into an average annual vehicle cost increase of $596, or about 4.9 cents more per vehicle mile of travel. Based on the cost-effectiveness analysis and assumptions, there are currently no TxDOT stations suitable for conversion to compressed natural gas.

  4. The cytotoxic mechanism of karlotoxin 2 (KmTx 2) from Karlodinium veneficum (Dinophyceae)

    PubMed Central

    Deeds, Jonathan R.; Hoesch, Robert E.; Place, Allen R.; Kao, Joseph P.Y.

    2015-01-01

    This study demonstrates that the polyketide toxin karlotoxin 2 (KmTx 2) produced by Karlodinium veneficum, a dinoflagellate associated with fish kills in temperate estuaries worldwide, alters vertebrate cell membrane permeability. Microfluorimetric and electrophysiological measurements were used to determine that vertebrate cellular toxicity occurs through non-selective permeabilization of plasma membranes, leading to osmotic cell lysis. Previous studies showed that KmTx 2 is lethal to fish at naturally-occurring concentrations measured during fish kills, while sub-lethal doses severely damage gill epithelia. This study provides a mechanistic explanation for the association between K. veneficum blooms and fish kills that has long been observed in temperate estuaries worldwide. PMID:25546005

  5. Disposal of hazardous materials from TxDOT activities. Final report, September 1992-August 1994

    SciTech Connect

    Stallard, M.; Corapcioglu, M.Y.; Beavers, T.; Beck, B.; Mehevec, A.

    1994-11-01

    The process of purchasing, storing, handling and disposal of hazardous waste is demanding. The Texas Department of Transportation deals with many such compounds every day in performing its duty of maintaining over 70,000 miles of Texas roadway. With the new demands being placed on all users of hazardous materials by the new EPA guidelines, procedures must be enacted to ensure TxDOT`s compliance with these ever-changing regulations. The placement of full-time safety and hazardous materials coordinators in each district office will help to ensure that employees follow reporting procedures and use disposal guidelines. The report will discuss these actions and others that might help TxDOT in this task.

  6. GOC-TX: A Reliable Ticket Synchronization Application for the Open Science Grid

    NASA Astrophysics Data System (ADS)

    Hayashi, Soichi; Gopu, Arvind; Quick, Robert

    2011-12-01

    One of the major operational issues faced by large multi-institutional collaborations is permitting its users and support staff to use their native ticket tracking environment while also exchanging these tickets with collaborators. After several failed attempts at email-parser based ticket exchanges, the OSG Operations Group has designed a comprehensive ticket synchronizing application. The GOC-TX application uses web-service interfaces offered by various commercial, open source and other homegrown ticketing systems, to synchronize tickets between two or more of these systems. GOC-TX operates independently from any ticketing system. It can be triggered by one ticketing system via email, active messaging, or a web-services call to check for current sync-status, pull applicable recent updates since prior synchronizations to the source ticket, and apply the updates to a destination ticket. The currently deployed production version of GOC-TX is able to synchronize tickets between the Numara Footprints ticketing system used by the OSG and the following systems: European Grid Initiative's system Global Grid User Support (GGUS) and the Request Tracker (RT) system used by Brookhaven. Additional interfaces to the BMC Remedy system used by Fermilab, and to other instances of RT used by other OSG partners, are expected to be completed in summer 2010. A fully configurable open source version is expected to be made available by early autumn 2010. This paper will cover the structure of the GOC-TX application, its evolution, and the problems encountered by OSG Operations group with ticket exchange within the OSG Collaboration.

  7. 77 FR 65360 - Foreign-Trade Zone 168-Dallas/Ft. Worth, TX; Notification of Proposed Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-26

    ... Production Activity, Richemont North America, Inc. dba Cartier (Eyewear Assembly/Kitting), Grand Prairie, TX...), located in Grand Prairie, Texas. The notification conforming to the requirements of the regulations of...

  8. 78 FR 11816 - Foreign-Trade Zone 168-Dallas/Ft. Worth, TX, Authorization of Production Activity, Richemont...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-20

    ... Activity, Richemont North America, Inc. dba Cartier (Eyewear Assembly/Kitting), Grand Prairie, TX On... North America, Inc. dba Cartier, within FTZ 168--Site 4, in Grand Prairie, Texas. ] The notification...

  9. EPA Announces 2015 ENERGY STAR Certified Manufacturing Plants, Allergan Pharmaceuticals facility in Waco, TX, among those recognized

    EPA Pesticide Factsheets

    DALLAS - (Feb. 24, 2016) The U.S. Environmental Protection Agency (EPA) announced today that the Allergan Pharmaceuticals facility in Waco, TX, is among the 70 manufacturing plants across the nation that achieved ENERGY STAR certification for their

  10. 78 FR 4125 - Foreign-Trade Zone 265-Conroe, TX; Authorization of Production Activity; Bauer Manufacturing, Inc...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-18

    ... Manufacturing, Inc. (Pile Drivers and Boring Machinery), Conroe, TX On September 12, 2012, the City of Conroe... Zones (FTZ) Board on behalf of Bauer Manufacturing, Inc., within FTZ 265--Site 1, in Conroe, Texas....

  11. Evidence for reduced lymphatic CSF absorption in the H-Tx rat hydrocephalus model

    PubMed Central

    Rammling, Matthias; Madan, Meenu; Paul, Leena; Behnam, Babak; Pattisapu, Jogi V

    2008-01-01

    Background There is mounting evidence that spinal fluid absorption takes place not only at the arachnoid villi, but also at several extracranial sites, which might serve as a reserve mechanism for, or be primarily involved in the absorption of CSF in hydrocephalus. Methods We compared the nasal lymphatic pathway in congenital Hydrocephalus-Texas (H-Tx) rats in unaffected and affected hydrocephalic (HC) siblings with that of control Sprague Dawley (SD) rat pups. The animals were examined after immediate post mortem injection of Evan's blue dye into the cisterna magna at 6 and 10 days of age. The specimens were evaluated for amount of dye penetration into the nasal passages. Results We found more dye visualization in the olfactory regions of control SD (14/16 at P6, 14/16 at P10) and unaffected H-Tx (13/17 at P6, 13/16 at P10) compared with HC animals (0/14 at P6, 3/15 at P10). This difference was more pronounced at 10 days of age. The dye was not visualized in the cervical lymph nodes or venous channels in these acute experiments. Conclusion The results of this study suggest that nasal lymphatic cerebrospinal fluid absorption is reduced in the H-Tx rat hydrocephalus model. PMID:18925964

  12. PhTx3-4, a Spider Toxin Calcium Channel Blocker, Reduces NMDA-Induced Injury of the Retina

    PubMed Central

    Binda, Nancy Scardua; Porto Petruceli Carayon, Charles; Agostini, Rafael Mourão; do Nascimento Pinheiro, Ana Cristina; Nascimento Cordeiro, Marta; Romano Silva, Marco Aurélio; Figueira Silva, Juliana; Rita Pereira, Elizete Maria; da Silva Junior, Claudio Antonio; de Castro Junior, Célio José; Sena Guimarães, Andre Luiz; Gomez, Marcus Vinicius

    2016-01-01

    The in vivo neuroprotective effect of PhTx3-4, a spider toxin N-P/Q calcium channel blocker, was studied in a rat model of NMDA-induced injury of the retina. NMDA (N-Methyl-d-Aspartate)-induced retinal injury in rats reduced the b-wave amplitude by 62% ± 3.6%, indicating the severity of the insult. PhTx3-4 treatment increased the amplitude of the b-wave, which was almost equivalent to the control retinas that were not submitted to injury. The PhTx3-4 functional protection of the retinas recorded on the ERG also was observed in the neuroprotection of retinal cells. NMDA-induced injury reduced live cells in the retina layers and the highest reduction, 84%, was in the ganglion cell layer. Notably, PhTx3-4 treatment caused a remarkable reduction of dead cells in the retina layers, and the highest neuroprotective effect was in the ganglion cells layer. NMDA-induced cytotoxicity of the retina increased the release of glutamate, reactive oxygen species (ROS) production and oxidative stress. PhTx3-4 treatment reduced glutamate release, ROS production and oxidative stress measured by malondialdehyde. Thus, we presented for the first time evidence of in vivo neuroprotection from NMDA-induced retinal injury by PhTx3-4 (-ctenitoxin-Pn3a), a spider toxin that blocks N-P/Q calcium channels. PMID:26978403

  13. Unmasking Snake Venom of Bothrops leucurus: Purification and Pharmacological and Structural Characterization of New PLA2 Bleu TX-III

    PubMed Central

    Marangoni, Fábio André; Ponce-Soto, Luis Alberto; Marangoni, Sergio; Landucci, Elen Cristina Teizem

    2013-01-01

    Bleu TX-III was isolated from Bothrops leucurus snake venom on one-step analytical chromatography reverse phase HPLC, was homogeneous on SDS-PAGE, and was confirmed by Q-Tof Ultima API ESI/MS (TOF MS mode) mass spectrometry in 14243.8 Da. Multiple alignments of Bleu TX-III show high degree of homology with basic PLA2 myotoxins from other Bothrops venoms. Our studies on local and systemic myotoxicity “in vivo” reveal that Bleu TX-III is myotoxin with local but not systemic action due to the decrease in the plasmatic CK levels when Bleu TX-III is administrated by intravenous route in mice (dose 1 and 5 μg). And at a dose of 20 μg myotoxin behaves like a local and systemic action. Bleu TX-III induced moderate marked paw edema, evidencing the local increase in vascular permeability. The inflammatory events induced in the mice (I.M.) were investigated. The increase in the levels of IL-1, IL-6, and TNF-α was observed in the plasma. It is concluded that Bleu TX-III induces inflammatory events in this model. The enzymatic phospholipid hydrolysis may be relevant to these phenomena. Bothrops leucurus venom is still not extensively explored, and the knowledge of its toxins separately through the study of structure/function will contribute for a better understanding of its action mechanism. PMID:23509815

  14. Receiver deghosting in the t-x domain based on super-Gaussianity

    NASA Astrophysics Data System (ADS)

    Lu, Wenkai; Xu, Ziqiang; Fang, Zhongyu; Wang, Ruiliang; Yan, Chengzhi

    2017-01-01

    Deghosting methods in the time-space (t-x) domain have attracted a lot of attention because of their flexibility for various source/receiver configurations. Based on the well-known knowledge that the seismic signal has a super-Gaussian distribution, we present a Super-Gaussianity based Receiver Deghosting (SRD) method in the t-x domain. In our method, we denote the upgoing wave and its ghost (downgoing wave) as a single seismic signal, and express the relationship between the upgoing wave and its ghost using two ghost parameters: the sea surface reflection coefficient and the time-shift between the upgoing wave and its ghost. For a single seismic signal, we estimate these two parameters by maximizing the super-Gaussianity of the deghosted output, which is achieved by a 2D grid search method using an adaptively predefined discrete solution space. Since usually a large number of seismic signals are mixed together in a seismic trace, in the proposed method we divide the seismic trace into overlapping frames using a sliding time window with a step of one time sample, and consider each frame as a replacement for a single seismic signal. For a 2D seismic gather, we obtain two 2D maps of the ghost parameters. By assuming that these two parameters vary slowly in the t-x domain, we apply a 2D average filter to these maps, to improve their reliability further. Finally, these deghosted outputs are merged to form the final deghosted result. To demonstrate the flexibility of the proposed method for arbitrary variable depths of the receivers, we apply it to several synthetic and field seismic datasets acquired by variable depth streamer.

  15. Catching the fish - Constraining stellar parameters for TX Piscium using spectro-interferometric observations

    NASA Astrophysics Data System (ADS)

    Klotz, D.; Paladini, C.; Hron, J.; Aringer, B.; Sacuto, S.; Marigo, P.; Verhoelst, T.

    2013-02-01

    Context. Stellar parameter determination is a challenging task when dealing with galactic giant stars. The combination of different investigation techniques has proven to be a promising approach. Aims: We analyse archive spectra obtained with the Short Wavelength Spectrometer (SWS) onboard ISO, and new interferometric observations from the Very Large Telescope MID-infrared Interferometric instrument (VLTI/MIDI) of a very well studied carbon-rich giant: TX Psc. The aim of this work is to determine stellar parameters using spectroscopy and interferometry. Methods: The observations are used to constrain the model atmosphere, and eventually the stellar evolutionary model in the region where the tracks map the beginning of the carbon star sequence. Two different approaches are used to determine stellar parameters: (i) the "classic" interferometric approach where the effective temperature is fixed by using the angular diameter in the N-band (from interferometry) and the apparent bolometric magnitude; (ii) parameters are obtained by fitting a grid of state-of-the-art hydrostatic models to spectroscopic and interferometric observations. Results: We find good agreement between the parameters of the two methods. The effective temperature and luminosity clearly place TX Psc in the carbon-rich AGB star domain in the H-R-diagram. Current evolutionary tracks suggest that TX Psc became a C-star just recently, which means that the star is still in a "quiet" phase compared to the subsequent strong-wind regime. This agrees with the C/O ratio being only slightly greater than one. Based on observations made with ESO telescopes at Paranal Observatory under program IDs 74.D-0601, 60.A-9224, 77.C-0440, 60.A-9006, 78.D-0112, 84.D-0805.

  16. Tank 241-TX-104, cores 230 and 231 analytical results for the final report

    SciTech Connect

    Diaz, L.A.

    1998-07-07

    This document is the analytical laboratory report for tank 241-TX-104 push mode core segments collected between February 18, 1998 and February 23, 1998. The segments were subsampled and analyzed in accordance with the Tank 241-TX-104 Push Mode Core Sampling and Analysis Plan (TSAP) (McCain, 1997), the Data Quality Objective to Support Resolution of the Organic Complexant Safety Issue (Organic DQO) (Turner, et al., 1995) and the Safety Screening Data Quality Objective (DQO) (Dukelow, et.al., 1995). The analytical results are included in the data summary table. None of the samples submitted for Differential Scanning Calorimetry (DSC) and Total Alpha Activity (AT) exceeded notification limits as stated in the TSAP. The statistical results of the 95% confidence interval on the mean calculations are provided by the Tank Waste Remediation Systems Technical Basis Group in accordance with the Memorandum of Understanding (Schreiber, 1997) and are not considered in this report. Appearance and Sample Handling Attachment 1 is a cross reference to relate the tank farm identification numbers to the 222-S Laboratory LabCore/LIMS sample numbers. The subsamples generated in the laboratory for analyses are identified in these diagrams with their sources shown. Core 230: Three push mode core segments were removed from tank 241-TX-104 riser 9A on February 18, 1998. Segments were received by the 222-S Laboratory on February 19, 1998. Two segments were expected for this core. However, due to poor sample recovery, an additional segment was taken and identified as 2A. Core 231: Four push mode core segments were removed from tank 241-TX-104 riser 13A between February 19, 1998 and February 23, 1998. Segments were received by the 222-S Laboratory on February 24, 1998. Two segments were expected for this core. However, due to poor sample recovery, additional segments were taken and identified as 2A and 2B. The TSAP states the core samples should be transported to the laboratory within three

  17. Chemical Modifications of PhTX-I Myotoxin from Porthidium hyoprora Snake Venom: Effects on Structural, Enzymatic, and Pharmacological Properties

    PubMed Central

    Huancahuire-Vega, Salomón; Corrêa, Daniel H. A.; Hollanda, Luciana M.; Lancellotti, Marcelo; Ramos, Carlos H. I.; Ponce-Soto, Luis Alberto; Marangoni, Sergio

    2013-01-01

    We recently described the isolation of a basic PLA2 (PhTX-I) from Porthidium hyoprora snake venom. This toxin exhibits high catalytic activity, induces in vivo myotoxicity, moderates footpad edema, and causes in vitro neuromuscular blockade. Here, we describe the chemical modifications of specific amino acid residues (His, Tyr, Lys, and Trp), performed in PhTX-I, to study their effects on the structural, enzymatic, and pharmacological properties of this myotoxin. After chemical treatment, a single His, 4 Tyr, 7 Lys, and one Trp residues were modified. The secondary structure of the protein remained unchanged as measured by circular dichroism; however other results indicated the critical role played by Lys and Tyr residues in myotoxic, neurotoxic activities and mainly in the cytotoxicity displayed by PhTX-I. His residue and therefore catalytic activity of PhTX-I are relevant for edematogenic, neurotoxic, and myotoxic effects, but not for its cytotoxic activity. This dissociation observed between enzymatic activity and some pharmacological effects suggests that other molecular regions distinct from the catalytic site may also play a role in the toxic activities exerted by this myotoxin. Our observations supported the hypothesis that both the catalytic sites as the hypothetical pharmacological sites are relevant to the pharmacological profile of PhTX-I. PMID:23484072

  18. Centipede venom peptide SsmTX-I with two intramolecular disulfide bonds shows analgesic activities in animal models.

    PubMed

    Wang, Ying; Li, Xiaojie; Yang, Meifeng; Wu, Chunyun; Zou, Zhirong; Tang, Jing; Yang, Xinwang

    2017-03-01

    Pain is a major symptom of many diseases and results in enormous pressures on human body or society. Currently, clinically used analgesic drugs, including opioids and nonsteroidal anti-inflammatory drugs, have adverse reactions, and thus, the development of new types of analgesic drug candidates is urgently needed. Animal venom peptides have proven to have potential as new types of analgesic medicine. In this research, we describe the isolation and characterization of an analgesic peptide from the crude venom of centipede, Scolopendra subspinipes mutilans. The amino acid sequence of this peptide was identical with SsmTX-I that was previously reported as a specific Kv2.1 ion channel blocker. Our results revealed that SsmTX-I was produced by posttranslational processing of a 73-residue prepropeptide. The intramolecular disulfide bridge motifs of SsmTX-I was Cys1-Cys3 and Cys2-Cys4. Functional assay revealed that SsmTX-I showed potential analgesic activities in formalin-induced paw licking, thermal pain, and acetic acid-induced abdominal writhing mice models. Our research provides the first report of cDNA sequences, disulfide motif, successful synthesis, and analgesic potential of SsmTX-I for the development of pain-killing drugs. It indicates that centipede peptide toxins could be a treasure trove for the search of novel analgesic drug candidates. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.

  19. Tank 241-TX-118, core 236 analytical results for the final report

    SciTech Connect

    ESCH, R.A.

    1998-11-19

    This document is the analytical laboratory report for tank 241-TX-118 push mode core segments collected between April 1, 1998 and April 13, 1998. The segments were subsampled and analyzed in accordance with the Tank 241-TX-118 Push Mode Core sampling and Analysis Plan (TSAP) (Benar, 1997), the Safety Screening Data Quality Objective (DQO) (Dukelow, et al., 1995), the Data Quality Objective to Support Resolution of the Organic Complexant Safety Issue (Organic DQO) (Turner, et al, 1995) and the Historical Model Evaluation Data Requirements (Historical DQO) (Sipson, et al., 1995). The analytical results are included in the data summary table (Table 1). None of the samples submitted for Differential Scanning Calorimetry (DSC) and Total Organic Carbon (TOC) exceeded notification limits as stated in the TSAP (Benar, 1997). One sample exceeded the Total Alpha Activity (AT) analysis notification limit of 38.4{micro}Ci/g (based on a bulk density of 1.6), core 236 segment 1 lower half solids (S98T001524). Appropriate notifications were made. Plutonium 239/240 analysis was requested as a secondary analysis. The statistical results of the 95% confidence interval on the mean calculations are provided by the Tank Waste Remediation Systems Technical Basis Group in accordance with the Memorandum of Understanding (Schreiber, 1997) and are not considered in this report.

  20. Texture enhanced optimization-based image reconstruction (TxE-OBIR) from sparse projection views

    NASA Astrophysics Data System (ADS)

    Xie, Huiqiao; Niu, Tianye; Yang, Yi; Ren, Yi; Tang, Xiangyang

    2016-03-01

    The optimization-based image reconstruction (OBIR) has been proposed and investigated in recent years to reduce radiation dose in X-ray computed tomography (CT) through acquiring sparse projection views. However, the OBIR usually generates images with a quite different noise texture compared to the clinical widely used reconstruction method (i.e. filtered back-projection - FBP). This may make the radiologists/physicians less confident while they are making clinical decisions. Recognizing the fact that the X-ray photon noise statistics is relatively uniform across the detector cells, which is enabled by beam forming devices (e.g. bowtie filters), we propose and evaluate a novel and practical texture enhancement method in this work. In the texture enhanced optimization-based image reconstruction (TxEOBIR), we first reconstruct a texture image with the FBP algorithm from a full set of synthesized projection views of noise. Then, the TxE-OBIR image is generated by adding the texture image into the OBIR reconstruction. As qualitatively confirmed by visual inspection and quantitatively by noise power spectrum (NPS) evaluation, the proposed method can produce images with textures that are visually identical to those of the gold standard FBP images.

  1. Monte Carlo modeling of a Novalis Tx Varian 6 MV with HD-120 multileaf collimator.

    PubMed

    Vazquez-Quino, Luis Alberto; Massingill, Brian; Shi, Chengyu; Gutierrez, Alonso; Esquivel, Carlos; Eng, Tony; Papanikolaou, Nikos; Stathakis, Sotirios

    2012-09-06

    A Monte Carlo model of the Novalis Tx linear accelerator equipped with high-definition multileaf collimator (HD-120 HD-MLC) was commissioned using ionization chamber measurements in water. All measurements in water were performed using a liquid filled ionization chamber. Film measurements were made using EDR2 film in solid water. Open rectangular fields defined by the jaws or the HD-MLC were used for comparison against measurements. Furthermore, inter- and intraleaf leakage calculated by the Monte Carlo model was compared against film measurements. The statistical uncertainty of the Monte Carlo calculations was less than 1% for all simulations. Results for all regular field sizes show an excellent agreement with commissioning data (percent depth-dose curves and profiles), well within 1% of difference in the relative dose and 1 mm distance to agreement. The computed leakage through HD-MLCs shows good agreement with film measurements. The Monte Carlo model developed in this study accurately represents the new Novalis Tx Varian linac with HD-MLC and can be used for reliable patient dose calculations.

  2. Tank characterization report for single-shell tank 241-TX-104

    SciTech Connect

    FIELD, J.G.

    1999-05-12

    A major function of the Tank Waste Remediation System (TWRS) is to characterize waste in support of waste management and disposal activities at the Hanford Site. Analytical data from sampling and analysis and other available information about a tank are compiled and maintained in a tank characterization report (TCR). This report and its appendices serve as the TCR for single-shell tank 241-TX-104. The objectives of this report are (1) to use characterization data in response to technical issues associated with tank 241-TX-104 waste, and (2) to provide a standard characterization of this waste in terms of a best-basis inventory estimate. Section 2.0 summarizes the response to technical issues, Section 3.0 shows the best-basis inventory estimate, Section 4.0 makes recommendations about the safety status of the tank and additional sampling needs. The appendices contain supporting data and information. This report supports the requirements of the Hanford Federal Facility Agreement and Consent Order (Ecology et al. 1997), Milestone M-44-15c, change request M-44-97-03 to ''issue characterization deliverables consistent with the Waste Information Requirements Document developed for FY 1999'' (Adams et al. 1998).

  3. Atomic defects in monolayer titanium carbide (Ti3C2Tx) MXene

    DOE PAGES

    Sang, Xiahan; Xie, Yu; Lin, Ming -Wei; ...

    2016-09-06

    Here, the 2D transition metal carbides or nitrides, or MXenes, are emerging as a group of materials showing great promise in lithium ion batteries and supercapacitors. Until now, characterization and properties of single-layer MXenes have been scarcely reported. Here, using scanning transmission electron microscopy, we determined the atomic structure of freestanding monolayer Ti3C2Tx flakes prepared via the minimally intensive layer delamination method and characterized different point defects that are prevalent in the monolayer flakes. We determine that the Ti vacancy concentration can be controlled by the etchant concentration during preparation. Density function theory-based calculations confirm the defect structures and predictmore » that the defects can influence the surface morphology and termination groups, but do not strongly influence the metallic conductivity. Using devices fabricated from single- and few-layer Ti3C2Tx MXene flakes, the effect of the number of layers in the flake on conductivity has been demonstrated.« less

  4. Characterization of brevetoxin (PbTx-3) exposure in neurons of the anoxia-tolerant freshwater turtle (Trachemys scripta).

    PubMed

    Cocilova, Courtney C; Milton, Sarah L

    2016-11-01

    Harmful algal blooms are increasing in frequency and extent worldwide and occur nearly annually off the west coast of Florida where they affect both humans and wildlife. The dinoflagellate Karenia brevis is a key organism in Florida red tides that produces a suite of potent neurotoxins collectively referred to as the brevetoxins (PbTx). Brevetoxins bind to and open voltage gated sodium channels (VGSC), increasing cell permeability in excitable cells and depolarizing nerve and muscle tissue. Exposed animals may thus show muscular and neurological symptoms including head bobbing, muscle twitching, paralysis, and coma; large HABs can result in significant morbidity and mortality of marine life, including fish, birds, marine mammals, and sea turtles. Brevetoxicosis however is difficult to treat in endangered sea turtles as the physiological impacts have not been investigated and the magnitude and duration of brevetoxin exposure are generally unknown. In this study we used the freshwater turtle Trachemys scripta as a model organism to investigate the effects of the specific brevetoxin PbTx-3 in the turtle brain. Primary turtle neuronal cell cultures were exposed to a range of PbTx-3 concentrations to determine excitotoxicity. Agonists and antagonists of voltage-gated sodium channels and downstream targets were utilized to confirm the toxin's mode of action. We found that turtle neurons are highly resistant to PbTx-3; while cell viability decreased in a dose dependent manner across PbTx-3 concentrations of 100-2000nM, the EC50 was significantly higher than has been reported in mammalian neurons. PbTx-3 exposure resulted in significant Ca(2+) influx, which could be fully abrogated by the VGSC antagonist tetrodotoxin, NMDA receptor blocker MK-801, and tetanus toxin, indicating that the mode of action in turtle neurons is the same as in mammalian cells. As both turtle and mammalian VGSCs have a high affinity for PbTx-3, we suggest that the high resistance of the turtle

  5. Protein Degradation in a TX-TL Cell-free Expression System Using ClpXP Protease

    DTIC Science & Technology

    2014-07-14

    INTRODUCTION An in vitro S30-based Escherichia coli expression system (“Transcription-Translation”, or “TX-TL”) has been developed as an...REFERENCES: 1.! Sun,!Z.Z.,!et!al.,!Protocols(for(Implementing(an( Escherichia ( Coli (Based(TX9TL( Cell9Free(Expression(System(for(Synthetic...protein( degradation(in(an( Escherichia ( coli (cell9free(expression(system.!J!Biol!Eng,!2010.! 4:!p.!9.! 4.! Shin,!J.!and!V.!Noireaux,!Efficient(cell9free

  6. PhTX-II a Basic Myotoxic Phospholipase A2 from Porthidium hyoprora Snake Venom, Pharmacological Characterization and Amino Acid Sequence by Mass Spectrometry

    PubMed Central

    Huancahuire-Vega, Salomón; Ponce-Soto, Luis Alberto; Marangoni, Sergio

    2014-01-01

    A monomeric basic PLA2 (PhTX-II) of 14149.08 Da molecular weight was purified to homogeneity from Porthidium hyoprora venom. Amino acid sequence by in tandem mass spectrometry revealed that PhTX-II belongs to Asp49 PLA2 enzyme class and displays conserved domains as the catalytic network, Ca2+-binding loop and the hydrophobic channel of access to the catalytic site, reflected in the high catalytic activity displayed by the enzyme. Moreover, PhTX-II PLA2 showed an allosteric behavior and its enzymatic activity was dependent on Ca2+. Examination of PhTX-II PLA2 by CD spectroscopy indicated a high content of alpha-helical structures, similar to the known structure of secreted phospholipase IIA group suggesting a similar folding. PhTX-II PLA2 causes neuromuscular blockade in avian neuromuscular preparations with a significant direct action on skeletal muscle function, as well as, induced local edema and myotoxicity, in mice. The treatment of PhTX-II by BPB resulted in complete loss of their catalytic activity that was accompanied by loss of their edematogenic effect. On the other hand, enzymatic activity of PhTX-II contributes to this neuromuscular blockade and local myotoxicity is dependent not only on enzymatic activity. These results show that PhTX-II is a myotoxic Asp49 PLA2 that contributes with toxic actions caused by P. hyoprora venom. PMID:25365526

  7. A draft whole genome sequence of “Candidatus Liberibacter asiaticus” strain TX2351 from Asian citrus psyllids in Texas, USA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The draft genome sequence of “Candidatus Liberibacter asiaticus” strain TX2351 collected from ACP in South Texas has been determined. The TX2351 genome is 1,252,043 bp in size with a 36.5% G+C content, encoding 1,184 predicted open reading frames and 51 RNA genes....

  8. The toxic milk mutation, tx, which results in a condition resembling Wilson disease in humans, is linked to mouse chromosome 8

    SciTech Connect

    Rauch, H.; Wells, A.J.

    1995-09-20

    Litters produced by females homozygous for the toxic milk mutation, tx/tx, display a syndrome that includes poor growth, hypopigmentation, tremors, and death before 2 weeks of age. These features, consistent with copper deficiency, are attributed to failure in fetal hepatic copper accumulation. Here we report the chromosomal linkage of this mutation. 5 refs., 1 tab.

  9. 78 FR 19985 - Modification of VOR Federal Airways V-68, V-76, V-194, and V-548 in the Vicinity of Houston, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-03

    ... Federal Aviation Administration 14 CFR Part 71 RIN 2120-AA66 Modification of VOR Federal Airways V-68, V-76, V-194, and V-548 in the Vicinity of Houston, TX AGENCY: Federal Aviation Administration (FAA... (VOR) Federal airways V-68, V-76, V-194, and V-548 in the vicinity of Houston, TX. The FAA is...

  10. The effect of PhTx3 on the release of 3H-acetylcholine induced by tityustoxin and potassium in brain cortical slices and myenteric plexus.

    PubMed

    Gomez, R S; Casali, T A; Romano-Silva, M A; Cordeiro, M N; Diniz, C R; Moraes-Santos, T; Prado, M A; Gomez, M V

    1995-08-18

    The venom of the Brazilian spider Phoneutria nigriventer possesses several neurotoxic polypeptidic fractions. Previous work has established that one of the toxic components, PhTx3, inhibited Ca(2+)-dependent glutamate release and the increase in cytosolic free Ca2+ in response to membrane depolarization. In the present work, we investigated the effect of PhTx3 on the release of acetylcholine (ACh) from brain and peripheral neurons. PhTx3 decreased the release of [3H]-ACh induced by tityustoxin and KCl in brain cortical slices and myenteric plexus. The inhibitory effect of myenteric plexus had the same magnitude as that obtained in the absence of extracellular Ca2+. However, in brain PhTx3 was less efficient at decreasing the evoked release of ACh. These experiments suggest that the target of PhTx3 is coupled to the process of release of ACh in brain and autonomic nervous system.

  11. Cost-effectiveness analysis of TxDOT CNG fleet conversion, volume 1

    NASA Astrophysics Data System (ADS)

    Euritt, M. A.; Taylor, D. B.; Mahmassani, H.

    1992-08-01

    Increased emphasis on energy efficiency and air quality has resulted in a number of state and federal initiatives examining the use of alternative fuels for motor vehicles. A Texas program for alternate fuels includes compressed natural gas (CNG). Based on analysis of 30-year life-cycle costs, development of a natural gas vehicle (NGV) program for the Texas Department of Transportation (TxDOT) would cost about $47 million (in 1991 dollars). These costs include savings from lower-priced natural gas, infrastructure costs for a fast-fueling station, vehicle costs, and operating costs. The 30-year life-cycle costs translate into an average annual vehicle cost increase of $596, or about 4.9 cents more per vehicle mile of travel.

  12. Preliminary tank characterization report for single-shell tank 241-TX-111: Best-basis inventory

    SciTech Connect

    Place, D.E.

    1997-08-26

    An effort is underway to provide waste inventory estimates that will serve as standard characterization source terms for the various waste management activities. As part of this effort, an evaluation of available information for single-shell tank 241-TX-111 was performed, and a best-basis inventory was established. This work follows the methodology that was established by the standard inventory task. The best-basis inventory is based on an engineering assessment of waste type, process flowsheet data, early sample data, and/or other available information. The Standard Inventories of Chemicals and Radionuclides in Hanford Site Tank Wastes (Kupfer et al. 1997) describes standard methodology used to derive the tank-by-tank best-basis inventories. This preliminary TCR will be updated using this same methodology when additional data on tank contents become available.

  13. Preliminary tank characterization report for single-shell tank 241-TX-101: best-basis inventory

    SciTech Connect

    Kupfer, M.J.

    1997-09-02

    This document is a preliminary Tank Characterization Report (TCR). It only contains the current best-basis inventory (Appendix D) for single-shell tank 241-TX-101. No TCRs have been previously issued for this tank, and current core sample analyses are not available. The best-basis inventory, therefore, is based on an engineering assessment of waste type, process flowsheet data, early sample data, and/or other available information. The Standard Inventories of Chemicals and Radionuclides in Hanford Site Tank Wastes describes standard methodology used to derive the tank-by-tank best-basis inventories. This preliminary TCR will be updated using this same methodology when additional data on tank contents become available.

  14. Preliminary tank characterization report for single-shell tank 241-TX-106: Best-basis inventory

    SciTech Connect

    Kupfer, M.J.

    1997-08-26

    An effort is underway to provide waste inventory estimates that will serve as standard characterization source terms for the various waste management activities. As part of this effort, an evaluation of available information for single-shell tank 241-TX-106 was performed, and a best-basis inventory was established. This work follows the methodology that was established by the standard inventory task. The best-basis inventory is based on an engineering assessment of waste type, process flowsheet data, early sample data, and/or other available information. The Standard Inventories of Chemicals and Radionuclides in Hanford Site Tank Wastes (Kupfer et al. 1997) describes standard methodology used to derive the tank-by-tank best-basis inventories. This preliminary TCR will be updated using this same methodology when additional data on tank contents become available.

  15. Preliminary tank characterization report for single-shell tank 241-TX-112: Best-basis inventory

    SciTech Connect

    Place, D.E.

    1997-08-26

    An effort is underway to provide waste inventory estimates that will serve as standard characterization source terms for the various waste management activities. As part of this effort, an evaluation of available information for single-shell tank 241-TX-112 was performed, and a best-basis inventory was established. This work follows the methodology that was established by the standard inventory task. The best-basis inventory is based on an engineering assessment of waste type, process flowsheet data, early sample data, and other available information. The Standard Inventories of Chemicals and Radionuclides in Hanford Site Tank Wastes (Kupfer et al. 1997) describes standard methodology used to derive the tank-by-tank best-basis inventories. This preliminary TCR will be updated using this same methodology when additional data on tank contents become available.

  16. Preliminary tank characterization report for single-shell tank 241-TX-113: best-basis inventory

    SciTech Connect

    Place, D.E.

    1997-08-26

    This document is a preliminary Tank Characterization Report (TCR). It only contains the current best-basis inventory (Appendix D) for single-shell tank 241-TX-113. No TCRs have been previously issued for this tank, and current core sample analyses are not available. The best-basis inventory, therefore, is based on an engineering assessment of waste type, process flowsheet data, early sample data, and/or other available information. The Standard Inventories of Chemicals and Radionuclides in Hanford Site Tank Wastes describes standard methodology used to derive the tank-by-tank best-basis inventories. This preliminary TCR will be updated using this same methodology when additional data on tank contents become available.

  17. Preliminary tank characterization report for single-shell tank 241-TX-108: best-basis inventory

    SciTech Connect

    Jo, J.

    1997-08-26

    This document is a preliminary Tank Characterization Report (TCR). It only contains the current best-basis inventory (Appendix D) for single-shell tank 241-TX-108. No TCR has been previously issued for this tank, and current core sample analyses are not available. The best-basis inventory, therefore, is based on an engineering assessment of waste type, process flowsheet data, and/or other available information. The Standard Inventories of Chemicals and Radionuclides in Hanford Site Tank Wastes describes standard methodology used to derive the tank-by-tank best-basis inventories. This preliminary TCR will be updated using this same methodology when additional data on tank contents become available.

  18. 76 FR 33333 - Use of Small Area Fair Market Rents for Project Base Vouchers in the Dallas TX Metropolitan Area

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-08

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT Use of Small Area Fair Market Rents for Project Base Vouchers in the Dallas TX Metropolitan Area AGENCY: Office of the Assistant Secretary for Public and Indian Housing, HUD. ACTION:...

  19. 77 FR 8806 - Foreign-Trade Zone 183-Austin, TX; Application for Reorganization Under the Alternative Site...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-15

    ... Foreign-Trade Zones Board Foreign-Trade Zone 183--Austin, TX; Application for Reorganization Under the... within the Austin Enterprise Zone, located at Bolm Road and Gardner Road, Austin; Site 2 (50 acres)--Balcones Research site located in north central Austin at the intersection of Burnett Road and...

  20. 77 FR 31308 - Foreign-Trade Zone 36-Galveston, TX; Application for Reorganization/Expansion Under Alternative...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-25

    ... Foreign-Trade Zones Board Foreign-Trade Zone 36--Galveston, TX; Application for Reorganization/Expansion...) Board (the Board) by the Board of Trustees of the Galveston Wharves, grantee of FTZ 36, requesting... of Trustees of the Galveston Wharves (Board Order 1080, 65 FR 11548-11549, 3/3/00). The current...

  1. 75 FR 64276 - Reorganization/Expansion of Foreign-Trade Zone 196 Under Alternative Site Framework Fort Worth, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-19

    ... Framework Fort Worth, TX Pursuant to its authority under the Foreign-Trade Zones Act of June 18, 1934, as..., the Board adopted the alternative site framework (ASF) in December 2008 (74 FR 1170, 01/12/09... framework is approved, subject to the FTZ Act and the Board's regulations, including Section 400.28, to...

  2. 78 FR 73109 - Radio Broadcasting Services; Benjamin and Cisco, TX; De Beque, CO; Port Lions, AK; Rule and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-05

    ... COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Benjamin and Cisco, TX; De Beque, CO; Port Lions, AK... Commission amends the Table of FM Allotments by removing Channel 221C0 at Port Lions, Alaska; Channel 247C3... two or more FM auctions. No bids were entered for these allotments in Auction 94. It is...

  3. RCRA Assessment Plan for Single-Shell Tank Waste Management Area TX-TY at the Hanford Site

    SciTech Connect

    Hodges, Floyd N.; Chou, Charissa J.

    2001-02-23

    A groundwater quality assessment plan was prepared to investigate the rate and extent of aquifer contamination beneath Waste Management Area TX-TY on the Hanford Site in Washington State. This plan is an update of a draft plan issued in February 1999, which guided work performed in fiscal year 2000.

  4. 33 CFR 165.T08-0240 - Safety Zone; Kemah Boardwalk Summer Season Fireworks, Galveston Bay, Kemah, TX.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Summer Season Fireworks, Galveston Bay, Kemah, TX. 165.T08-0240 Section 165.T08-0240 Navigation and... Areas Eighth Coast Guard District § 165.T08-0240 Safety Zone; Kemah Boardwalk Summer Season Fireworks...' radius around a fireworks barge located at approximate Latitude 29°32′57″ N, Longitude 095°00′31″ W....

  5. Dielectric spectroscopy study on ionic liquid microemulsion composed of water, TX-100, and BmimPF6

    NASA Astrophysics Data System (ADS)

    Chen, Zhen; Nozaki, Ryusuke

    2012-06-01

    We report here a broadband dielectric spectroscopy study on an ionic liquid microemulsion (ILM) composed of water, Triton X-100 (TX-100), and 1-butyl-3-methylimidazolium hexafluorophosphate (bmimPF6). It is found that the phase behavior of this ILM can be easily identified by its dielectric response. The dielectric behavior of the ILM in the GHz range is consistent with that of TX-100/water mixtures with comparable water-to-TX-100 weight ratio. It consists of the relaxations due to ethylene oxide (EO) unit relaxation, hydration water dynamics, and/or free water dynamics. The water content dependence of the EO unit relaxation suggests that this relaxation involves dynamics of hydration water molecules. In the IL-in-water microemulsion phase, it is found that bmimPF6 molecules are preferentially dissolved in water when their concentration in water is lower than the solubility. An additional dielectric relaxation that is absent in the TX-100/water mixtures is observed in the frequency range of 107-108 Hz for this ILM. This low-frequency relaxation is found closely related to the bmimPF6 molecule and could be attributed to the hopping of its cations/anions between the anionic/cationic sites.

  6. 75 FR 29695 - Security Zones; Sabine Bank Channel, Sabine Pass Channel and Sabine-Neches Waterway, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-27

    ... Guard proposes a 100-foot security zone around LNG carriers while they are moored at the Golden Pass LNG facility in Sabine, TX and/or the Sabine Pass LNG facility located in Cheniere, LA. DATES: Comments and... the Captain of the Port to create fixed security zones around moored LNG carriers and moving...

  7. 75 FR 8741 - Notice of Intent to Repatriate Cultural Items: Stephen F. Austin State University, Nacogdoches, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-25

    ... National Park Service Notice of Intent to Repatriate Cultural Items: Stephen F. Austin State University... to repatriate cultural items in the control of Stephen F. Austin State University, Nacogdoches, TX... under contract with Stephen F. Austin State University. In 1957, 15 cultural items were removed from...

  8. Draft Genome Sequence of Pseudomonas nitroreducens Strain TX1, Which Degrades Nonionic Surfactants and Estrogen-Like Alkylphenols

    PubMed Central

    Chen, Hsin; Hu, Anyi; Tuan, Nguyen Ngoc

    2014-01-01

    Pseudomonas nitroreducens TX1 ATCC PTA-6168 was isolated from rice field drainage in Taiwan. The bacterium is of special interest because of its capability to use nonionic surfactants (alkylphenol polyethoxylates) and estrogen-like compounds (4-t-octylphenol and 4-nonylphenol) as a sole carbon source. This is the first report on the genome sequence of P. nitroreducens. PMID:24482523

  9. Balanced Reciprocal Translocation t(X;1) in a Girl with Tall Stature and Primary Amenorrhea

    PubMed Central

    Razavi, Zahra; Momtaz, Hossein Emad

    2017-01-01

    Chromosomal translocations constitute one of the most important, yet uncommon, causes of primary amenorrhea and gonadal dysgenesis. Although X-autosome translocations are frequently associated with streak gonads and clinical features of the Turner syndrome, the majority of X-autosome carriers may present with a variable phenotype, developmental delay, and recognizable X-linked syndrome due to nonrandom X-inactivation. In this article, we describe a healthy 15.5-year-old girl with primary amenorrhea, gonadal dysgenesis, and tall stature without other manifestations of the Turner syndrome. Relevant clinical, biochemical, endocrinological, and cytogenetical evaluations were performed. Initial investigations revealed hypergonadotropic hypogonadism (FSH=134 mIU/mL [normal=10–15 mIU/mL], LH=47.5 [normal=10–15 mIU/mL], and estradiol=24.3 pmol/L). On ultrasound examination of the pelvis, streak ovaries with a hypoplastic uterus were noted. Chromosome study, performed according to routine procedures, revealed an apparently balanced reciprocal translocation involving the short arm of chromosome 1(p2) and the long arm of the X chromosome (q2) in all the cells with the following karyotype: 46,X,t(1;X)(p13;q22). She was placed on hormone replacement therapy. In our patient, X-autosome translocation was associated with gonadal dysgenesis and tall stature. We conclude that t(X;1) may be associated with gonadal dysgenesis without other congenital abnormalities. To our knowledge, normal phenotype with gonadal dysgenesis and tall stature in association with t(X;1) translocation has not been previously reported. PMID:28360449

  10. High-Capacitance Mechanism for Ti3C2Tx MXene by in Situ Electrochemical Raman Spectroscopy Investigation.

    PubMed

    Hu, Minmin; Li, Zhaojin; Hu, Tao; Zhu, Shihao; Zhang, Chao; Wang, Xiaohui

    2016-12-27

    MXenes represent an emerging family of conductive two-dimensional materials. Their representative, Ti3C2Tx, has been recognized as an outstanding member in the field of electrochemical energy storage. However, an in-depth understanding of fundamental processes responsible for the superior capacitance of Ti3C2Tx MXene in acidic electrolytes is lacking. Here, to understand the mechanism of capacitance in Ti3C2Tx MXene, we studied electrochemically the charge/discharge processes of Ti3C2Tx electrodes in sulfate ion-containing aqueous electrolytes with three different cations, coupled with in situ Raman spectroscopy. It is demonstrated that hydronium in the H2SO4 electrolyte bonds with the terminal O in the negative electrode upon discharging while debonding occurs upon charging. Correspondingly, the reversible bonding/debonding changes the valence state of Ti element in the MXene, giving rise to the pseudocapacitance in the acidic electrolyte. In stark contrast, only electric double layer capacitance is recognized in the other electrolytes of (NH4)2SO4 or MgSO4. The charge storage ways also differ: ion exchange dominates in H2SO4, while counterion adsorption in the rest. Hydronium that is characterized by smaller hydration radius and less charge is the most mobile among the three cations, facilitating it more kinetically accommodated on the deep adsorption sites between the MXene layers. The two key factors, i.e., surface functional group-involved bonding/debonding-induced pseudocapacitance, and ion exchange-featured charge storage, simultaneously contribute to the superior capacitance of Ti3C2Tx MXene in acidic electrolytes.

  11. Nitric Oxide-Induced Vasorelaxation in Response to PnTx2-6 Toxin from Phoneutria nigriventer Spider in Rat Cavernosal Tissue

    PubMed Central

    Nunes, Kenia P.; Cordeiro, Marta N.; Richardson, Michael; Borges, Marcia N.; Diniz, Simone O.F.; Cardoso, Valbert N.; Tostes, Rita; De Lima, Maria Elena; Webb, Robert Clinton; Leite, Romulo

    2011-01-01

    Introduction Priapism is one of several symptoms observed in accidental bites by the spider Phoneutria nigriventer. The venom of this spider is comprised of many toxins, and the majority has been shown to affect excitable ion channels, mainly sodium (Na+) channels. It has been demonstrated that PnTx2-6, a peptide extracted from the venom of P. nigriventer, causes erection in anesthetized rats and mice. Aim We investigated the mechanism by which PnTx2-6 evokes relaxation in rat corpus cavernosum. Main Outcome Measures PnTx2-6 toxin potentiates nitric oxide (NO)-dependent cavernosal relaxation. Methods Rat cavernosal strips were incubated with bretylium (3 × 10−5 M) and contracted with phenylephrine (PE; 10−5 M). Relaxation responses were evoked by electrical field stimulation (EFS) or sodium nitroprusside (SNP) before and after 4 minutes of incubation with PnTx2-6 (10−8 M). The effect of PnTx2-6 on relaxation induced by EFS was also tested in the presence of atropine (10−6 M), a muscarinic receptor antagonist, N-type Ca2+ channel blockers (ω-conotoxin GVIA, 10−6 M) and sildenafil (3 × 10−8 M). Technetium99m radiolabeled PnTx2-6 subcutaneous injection was administrated in the penis. Results Whereas relaxation induced by SNP was not affected by PnTx2-6, EFS-induced relaxation was significantly potentiated by this toxin as well as PnTx2-6 plus SNP. This potentiating effect was further increased by sildenafil, not altered by atropine, however was completely blocked by the N-type Ca2+ channels. High concentrated levels of radiolabeled PnTx2-6 was specifically found in the cavernosum tissue, suggesting PnTx2-6 is an important toxin responsible for P. nigriventer spider accident-induced priapism. Conclusion We show that PnTx2-6 slows Na+ channels inactivation in nitrergic neurons, allowing Ca2+ influx to facilitate NO/cGMP signalling, which promotes increased NO production. In addition, this relaxation effect is independent of phosphodiesterase enzyme type 5

  12. The novel hypoxic cytotoxin, TX-2098 has antitumor effect in pancreatic cancer; possible mechanism through inhibiting VEGF and hypoxia inducible factor-1{alpha} targeted gene expression

    SciTech Connect

    Miyake, Kotaro; Nishioka, Masanori; Imura, Satoru; Batmunkh, Erdenebulgan; Uto, Yoshihiro; Nagasawa, Hideko; Hori, Hitoshi; Shimada, Mitsuo

    2012-08-01

    Tumor hypoxia has been considered to be a potential therapeutic target, because hypoxia is a common feature of solid tumors and is associated with their malignant phenotype. In the present study, we investigated the antitumor effect of a novel hypoxic cytotoxin, 3-[2-hydroxyethyl(methyl)amino]-2-quinoxalinecarbonitrile 1,4-dioxide (TX-2098) in inhibiting the expression of hypoxia inducible factor-1{alpha} (HIF-1{alpha}), and consequently vascular endothelial cell growth factor (VEGF) expression in pancreatic cancer. The antitumor effects of TX-2098 under hypoxia were tested against various human pancreatic cancer cell lines using WST-8 assay. VEGF protein induced pancreatic cancer was determined on cell-free supernatant by ELISA. Moreover, nude mice bearing subcutaneously (s.c.) or orthotopically implanted human SUIT-2 were treated with TX-2098. Tumor volume, survival and expression of HIF-1 and associated molecules were evaluated in treatment versus control groups. In vitro, TX-2098 inhibited the proliferation of various pancreatic cancer cell lines. In s.c model, tumors from nude mice injected with pancreatic cancer cells and treated with TX-2098 showed significant reductions in volume (P < 0.01 versus control). Quantitative real-time reverse transcription-PCR analysis revealed that TX-2098 significantly inhibited mRNA expression of the HIF-1 associated molecules, VEGF, glucose transporter 1 and Aldolase A (P < 0.01 versus control). These treatments also prolong the survival in orthotopic models. These results suggest that the effect of TX-2098 in pancreatic cancer might be correlated with the expression of VEGF and HIF-1 targeted molecules. -- Highlights: Black-Right-Pointing-Pointer We designed and synthesized novel hypoxic cytoxin, TX-2098. Black-Right-Pointing-Pointer TX-2098 inhibited the proliferation of human pancreatic cancer cells than TPZ. Black-Right-Pointing-Pointer TX-2098 reduced VEGF protein level than TPZ. Black-Right-Pointing-Pointer TX-2098

  13. TX model: a quantitative heat-loss analysis of district heating pipes by means of IR surface-temperature measurements

    NASA Astrophysics Data System (ADS)

    Zinko, Heimo; Perers, Bengt

    1995-03-01

    The aim of this study is to investigate the possibility of analyzing the temperature profile at the ground surface above buried district heating pipes in such a way that enables the quantitative determination of heat loss from the pair of pipes. In practical applications, it is supposed that this temperature profile is generated by means of thermography. For this purpose, the principle of the TX-model has been developed, implementing that the heat losses from pipes buried in the ground has a temperature signature on the ground surface. A qualitative analysis of this temperature signature is very well known and in practical use for detecting leaks from pipes. These techniques mostly makes use of relative changes of the temperature pattern along the pipe. In the quantitative heat loss analysis, however, it is presumed that the temperature profile across the pipes is related to the pipe heat loss in Watt/m. The basic idea is that the integral of the temperature variation across the pipe, called TX, is a function of the heat loss, but affected by some other parameters such as depth, heat diffusivity and so on. In order to analyze the parameters influencing the TX-factor, a simulation model for the energy balance at the ground surface has been developed. This model includes the heat flow from the pipe to the surface and the heat exchange at the surface with the environment due to convection, latent heat change, solar and long wave radiation. The simulation gives the surprising result that the TX factor is relatively unaffected during the course of a day even when the sun is shining, as long as other climate conditions are relatively stable (low wind, no rain, no shadows). The results from the simulations were verified at a testfield in Studsvik, Sweden, with electrically controlled pipe heat losses and long term monitoring of the surface temperature profile and TX factor with temperature sensors at the ground surface. The quantitative TX model for heat loss

  14. Effects of Tityus serrulatus scorpion venom and its toxin TsTX-V on neurotransmitter uptake in vitro

    SciTech Connect

    Cecchini, Alessandra L.; Vasconcelos, Flavio; Giglio, Jose Roberto; Arantes, Eliane Candiani . E-mail: ecabraga@fcfrp.usp.br

    2006-12-01

    Scorpion neurotoxins targeting the Na{sub v} channel can be classified into two classes: {alpha}- and {beta}-neurotoxins and are reported as highly active in mammalian brain. In this work, we evaluate the effects of Tityus serrulatus venom (Ts venom) and its {alpha}-neurotoxin TsTX-V on {gamma}-aminobutyric acid (GABA), dopamine (DA) and glutamate (Glu) uptake in isolated rat brain synaptosomes. TsTX-V was isolated from Ts venom by ion exchange chromatography followed by reverse-phase (C18) high-performance liquid chromatography. Neither Ts venom nor TsTX-V was able to affect {sup 3}H-Glu uptake. On the other hand, Ts venom (0.13 {mu}g/mg) significantly inhibited both {sup 3}H-GABA and {sup 3}H-DA uptake ({approx} 50%). TsTX-V showed IC{sub 5} values of 9.37 {mu}M and 22.2 {mu}M for the inhibition of {sup 3}H-GABA and {sup 3}H-DA uptake, respectively. These effects were abolished by pre-treatment with tetrodotoxin (TTX, 1 {mu}M), indicating the involvement of voltage-gated Na{sup +} channels in this process. In the absence of Ca{sup 2+}, and at low Ts venom concentrations, the reduction of {sup 3}H-GABA uptake was not as marked as in the presence of Ca{sup 2+}. TsTX-V did not reduce {sup 3}H-GABA uptake in COS-7 cells expressing the GABA transporters GAT-1 and GAT-3, suggesting that this toxin indirectly reduces the transport. The reduced {sup 3}H-GABA uptake by synaptosomes might be due to rapid cell depolarization as revealed by confocal microscopy of C6 glioma cells. Thus, TsTX-V causes a reduction of {sup 3}H-GABA and {sup 3}H-DA uptake in a Ca{sup 2+}-dependent manner, not directly affecting GABA transporters, but, in consequence of depolarization, involving voltage-gated Na{sup +} channels.

  15. Evaluation of integrated respiratory gating systems on a Novalis Tx system.

    PubMed

    Chang, Zheng; Liu, Tonghai; Cai, Jing; Chen, Qing; Wang, Zhiheng; Yin, Fang-Fang

    2011-04-04

    The purpose of this study was to investigate the accuracy of motion tracking and radiation delivery control of integrated gating systems on a Novalis Tx system. The study was performed on a Novalis Tx system, which is equipped with Varian Real-time Position Management (RPM) system, and BrainLAB ExacTrac gating systems. In this study, the two systems were assessed on accuracy of both motion tracking and radiation delivery control. To evaluate motion tracking, two artificial motion profiles and five patients' respiratory profiles were used. The motion trajectories acquired by the two gating systems were compared against the references. To assess radiation delivery control, time delays were measured using a single-exposure method. More specifically, radiation is delivered with a 4 mm diameter cone within the phase range of 10%-45% for the BrainLAB ExacTrac system, and within the phase range of 0%-25% for the Varian RPM system during expiration, each for three times. Radiochromic films were used to record the radiation exposures and to calculate the time delays. In the work, the discrepancies were quantified using the parameters of mean and standard deviation (SD). Pearson's product-moment correlational analysis was used to test correlation of the data, which is quantified using a parameter of r. The trajectory profiles acquired by the gating systems show good agreement with those reference profiles. A quantitative analysis shows that the average mean discrepancies between BrainLAB ExacTrac system and known references are 1.5 mm and 1.9 mm for artificial and patient profiles, with the maximum motion amplitude of 28.0 mm. As for the Varian RPM system, the corresponding average mean discrepancies are 1.1 mm and 1.7 mm for artificial and patient profiles. With the proposed single-exposure method, the time delays are found to be 0.20 ± 0.03 seconds and 0.09 ± 0.01 seconds for BrainLAB ExacTrac and Varian RPM systems, respectively. The results indicate the systems can

  16. The complex environment of the bright carbon star TX Piscium as probed by spectro-astrometry

    NASA Astrophysics Data System (ADS)

    Hron, J.; Uttenthaler, S.; Aringer, B.; Klotz, D.; Lebzelter, T.; Paladini, C.; Wiedemann, G.

    2015-12-01

    Context. Stars on the asymptotic giant branch (AGB) show broad evidence of inhomogeneous atmospheres and circumstellar envelopes. These have been studied by a variety of methods on various angular scales. In this paper we explore the envelope of the well-studied carbon star TX Psc by the technique of spectro-astrometry. Aims: We explore the potential of this method for detecting asymmetries around AGB stars. Methods: We obtained CRIRES observations of several CO Δv = 1 lines near 4.6 μm and HCN lines near 3 μm in 2010 and 2013. These were then searched for spectro-astrometric signatures. For the interpretation of the results, we used simple simulated observations. Results: Several lines show significant photocentre shifts with a clear dependence on position angle. In all cases, tilde-shaped signatures are found where the positive and negative shifts (at PA 0°) are associated with blue and weaker red components of the lines. The shifts can be modelled with a bright blob 70 mas to 210 mas south of the star with a flux of several percent of the photospheric flux. We estimate a lower limit of the blob temperature of 1000 K. The blob may be related to a mass ejection as found for AGB stars or red supergiants. We also consider the scenario of a companion object. Conclusions: Although there is clear spectro-astrometric evidence of a rather prominent structure near TX Psc, it does not seem to relate to the other evidence of asymmetries, so no definite explanation can be given. Our data thus underline the very complex structure of the environment of this star, but further observations that sample the angular scales out to a few hundred milli-arcseconds are needed to get a clearer picture. Based on observations made with ESO telescopes at La Silla Paranal Observatory under programme IDs 386.D-0091 and 091.D-0094.Appendix A is available in electronic form at http://www.aanda.org

  17. Rapidly characterizing the fast dynamics of RNA genetic circuitry with cell-free transcription-translation (TX-TL) systems.

    PubMed

    Takahashi, Melissa K; Chappell, James; Hayes, Clarmyra A; Sun, Zachary Z; Kim, Jongmin; Singhal, Vipul; Spring, Kevin J; Al-Khabouri, Shaima; Fall, Christopher P; Noireaux, Vincent; Murray, Richard M; Lucks, Julius B

    2015-05-15

    RNA regulators are emerging as powerful tools to engineer synthetic genetic networks or rewire existing ones. A potential strength of RNA networks is that they may be able to propagate signals on time scales that are set by the fast degradation rates of RNAs. However, a current bottleneck to verifying this potential is the slow design-build-test cycle of evaluating these networks in vivo. Here, we adapt an Escherichia coli-based cell-free transcription-translation (TX-TL) system for rapidly prototyping RNA networks. We used this system to measure the response time of an RNA transcription cascade to be approximately five minutes per step of the cascade. We also show that this response time can be adjusted with temperature and regulator threshold tuning. Finally, we use TX-TL to prototype a new RNA network, an RNA single input module, and show that this network temporally stages the expression of two genes in vivo.

  18. Rapidly Characterizing the Fast Dynamics of RNA Genetic Circuitry with Cell-Free Transcription–Translation (TX-TL) Systems

    PubMed Central

    2014-01-01

    RNA regulators are emerging as powerful tools to engineer synthetic genetic networks or rewire existing ones. A potential strength of RNA networks is that they may be able to propagate signals on time scales that are set by the fast degradation rates of RNAs. However, a current bottleneck to verifying this potential is the slow design-build-test cycle of evaluating these networks in vivo. Here, we adapt an Escherichia coli-based cell-free transcription-translation (TX-TL) system for rapidly prototyping RNA networks. We used this system to measure the response time of an RNA transcription cascade to be approximately five minutes per step of the cascade. We also show that this response time can be adjusted with temperature and regulator threshold tuning. Finally, we use TX-TL to prototype a new RNA network, an RNA single input module, and show that this network temporally stages the expression of two genes in vivo. PMID:24621257

  19. Photoinduced electron and energy transfer from coumarin 153 to perylenetetracarboxylic diimide in bmimPF6/TX-100/water microemulsions.

    PubMed

    Wu, Haixia; Wang, Haixia; Xue, Lin; Li, Xiyou

    2011-01-15

    A perylenetetracarboxylic diimide (PDI) compound with an attached hydrophilic polyoxyethylene group at the imide nitrogen position was designed and synthesized. Photoinduced electron and energy transfer between coumarin 153 (C-153) and PDI in a ternary microemulsion with an ionic liquid (bmimPF(6)/TX-100/H(2)O) were investigated by steady state electronic absorption and fluorescence spectroscopy. The results revealed that both PDI and C-153 resided at the interface between the surfactant TX-100 and the ionic liquid bmimPF(6) in the ternary microemulsions. The absorption spectra suggested no interactions between C-153 and PDI in the ground states, but the fluorescence spectra revealed the presence of an efficient electron transfer and a less efficient energy transfer from C-153 to PDI. Moreover, the electron transfer was much more efficient in microemulsions than that in homogeneous conventional organic solvents due to the unique micro-environment of the microemulsion.

  20. Reservoir fracture mapping using microearthquakes: Austin chalk, Giddings field, TX and 76 field, Clinton Co., KY

    SciTech Connect

    Phillips, W.S.; Rutledge, J.T.; Fairbanks, T.D.

    1996-12-31

    Patterns of microearthquakes detected downhole defined fracture orientation and extent in the Austin chalk, Giddings field, TX and the 76 field, Clinton Co., KY. We collected over 480 and 770 microearthquakes during hydraulic stimulation at two sites in the Austin chalk, and over 3200 during primary production in Clinton Co. Data were of high enough quality that 20%, 31% and 53% of the events could be located, respectively. Reflected waves constrained microearthquakes to the stimulated depths at the base of the Austin chalk. In plan view, microearthquakes defined elongate fracture zones extending from the stimulation wells parallel to the regional fracture trend. However, widths of the stimulated zones differed by a factor of live between the two Austin chalk sites, indicating a large difference in the population of ancillary fractures. Post-stimulation production was much higher from the wider zone. At Clinton Co., microearthquakes defined low-angle, reverse-fault fracture zones above and below a producing zone. Associations with depleted production intervals indicated the mapped fractures had been previously drained. Drilling showed that the fractures currently contain brine. The seismic behavior was consistent with poroelastic models that predicted slight increases in compressive stress above and below the drained volume.

  1. Reservoir fracture mapping using microearthquakes: Austin chalk, Giddings field, TX and 76 field, Clinton Co., KY

    SciTech Connect

    Phillips, W.S.; Rutledge, J.T.; Gardner, T.L.; Fairbanks, T.D.; Miller, M.E.; Schuessler, B.K.

    1996-11-01

    Patterns of microearthquakes detected downhole defined fracture orientation and extent in the Austin chalk, Giddings field, TX and the 76 field, Clinton Co., KY. We collected over 480 and 770 microearthquakes during hydraulic stimulation at two sites in the Austin chalk, and over 3200 during primary production in Clinton Co. Data were of high enough quality that 20%, 31% and 53% of the events could be located, respectively. Reflected waves constrained microearthquakes to the stimulated depths at the base of the Austin chalk. In plan view, microearthquakes defined elongate fracture zones extending from the stimulation wells parallel to the regional fracture trend. However, widths of the stimulated zones differed by a factor of five between the two Austin chalk sites, indicating a large difference in the population of ancillary fractures. Post-stimulation production was much higher from the wider zone. At Clinton Co., microearthquakes defined low-angle, reverse-fault fracture zones above and below a producing zone. Associations with depleted production intervals indicated the mapped fractures had been previously drained. Drilling showed that the fractures currently contain brine. The seismic behavior was consistent with poroelastic models that predicted slight increases in compressive stress above and below the drained volume.

  2. Nanoscale elastic changes in 2D Ti3C2Tx (MXene) pseudocapacitive electrodes

    DOE PAGES

    Come, Jeremy; Xie, Yu; Naguib, Michael; ...

    2016-02-01

    Designing sustainable electrodes for next generation energy storage devices relies on the understanding of their fundamental properties at the nanoscale, including the comprehension of ions insertion into the electrode and their interactions with the active material. One consequence of ion storage is the change in the electrode volume resulting in mechanical strain and stress that can strongly affect the cycle life. Therefore, it is important to understand the changes of dimensions and mechanical properties occurring during electrochemical reactions. While the characterization of mechanical properties via macroscopic measurements is well documented, in-situ characterization of their evolution has never been achieved atmore » the nanoscale. Two dimensional (2D) carbides, known as MXenes, are promising materials for supercapacitors and various kinds of batteries, and understating the coupling between their mechanical and electrochemical properties is therefore necessary. Here we report on in-situ imaging, combined with density functional theory of the elastic changes, of a 2D titanium carbide (Ti3C2Tx) electrode in direction normal to the basal plane during cation intercalation. The results show a strong correlation between the Li+ ions content and the elastic modulus, whereas little effects of K+ ions are observed. Moreover, this strategy enables identifying the preferential intercalation pathways within a single particle.« less

  3. Protocols for implementing an Escherichia coli based TX-TL cell-free expression system for synthetic biology.

    PubMed

    Sun, Zachary Z; Hayes, Clarmyra A; Shin, Jonghyeon; Caschera, Filippo; Murray, Richard M; Noireaux, Vincent

    2013-09-16

    Ideal cell-free expression systems can theoretically emulate an in vivo cellular environment in a controlled in vitro platform. This is useful for expressing proteins and genetic circuits in a controlled manner as well as for providing a prototyping environment for synthetic biology. To achieve the latter goal, cell-free expression systems that preserve endogenous Escherichia coli transcription-translation mechanisms are able to more accurately reflect in vivo cellular dynamics than those based on T7 RNA polymerase transcription. We describe the preparation and execution of an efficient endogenous E. coli based transcription-translation (TX-TL) cell-free expression system that can produce equivalent amounts of protein as T7-based systems at a 98% cost reduction to similar commercial systems. The preparation of buffers and crude cell extract are described, as well as the execution of a three tube TX-TL reaction. The entire protocol takes five days to prepare and yields enough material for up to 3000 single reactions in one preparation. Once prepared, each reaction takes under 8 hr from setup to data collection and analysis. Mechanisms of regulation and transcription exogenous to E. coli, such as lac/tet repressors and T7 RNA polymerase, can be supplemented. Endogenous properties, such as mRNA and DNA degradation rates, can also be adjusted. The TX-TL cell-free expression system has been demonstrated for large-scale circuit assembly, exploring biological phenomena, and expression of proteins under both T7- and endogenous promoters. Accompanying mathematical models are available. The resulting system has unique applications in synthetic biology as a prototyping environment, or "TX-TL biomolecular breadboard."

  4. Biochemical, Pharmacological, and Structural Characterization of New Basic PLA2 Bbil-TX from Bothriopsis bilineata Snake Venom

    PubMed Central

    Corasolla Carregari, Victor; Stuani Floriano, Rafael; Rodrigues-Simioni, Lea; Winck, Flavia V.; Baldasso, Paulo Aparecido; Ponce-Soto, Luis Alberto; Marangoni, Sergio

    2013-01-01

    Bbil-TX, a PLA2, was purified from Bothriopsis bilineata snake venom after only one chromatographic step using RP-HPLC on μ-Bondapak C-18 column. A molecular mass of 14243.8 Da was confirmed by Q-Tof Ultima API ESI/MS (TOF MS mode) mass spectrometry. The partial protein sequence obtained was then submitted to BLASTp, with the search restricted to PLA2 from snakes and shows high identity values when compared to other PLA2s. PLA2 activity was presented in the presence of a synthetic substrate and showed a minimum sigmoidal behavior, reaching its maximal activity at pH 8.0 and 25–37°C. Maximum PLA2 activity required Ca2+ and in the presence of Cd2+, Zn2+, Mn2+, and Mg2+ it was reduced in the presence or absence of Ca2+. Crotapotin from Crotalus durissus cascavella rattlesnake venom and antihemorrhagic factor DA2-II from Didelphis albiventris opossum sera under optimal conditions significantly inhibit the enzymatic activity. Bbil-TX induces myonecrosis in mice. The fraction does not show a significant cytotoxic activity in myotubes and myoblasts (C2C12). The inflammatory events induced in the serum of mice by Bbil-TX isolated from Bothriopsis bilineata snake venom were investigated. An increase in vascular permeability and in the levels of TNF-a, IL-6, and IL-1 was was induced. Since Bbil-TX exerts a stronger proinflammatory effect, the phospholipid hydrolysis may be relevant for these phenomena. PMID:23509754

  5. The Close Environment of the C-star TX Psc as Seen with CRIRES Spectro-Astrometry

    NASA Astrophysics Data System (ADS)

    Hron, J.; Lebzelter, T.; Paladini, C.; Uttenthaler, S.; Aringer, B.; Wiedemann, G.

    2015-08-01

    We present CRIRES observations of the "normal" carbon star TX Psc in the K, L and M bands, taken in 2010 and 2013. The spectro-astrometry shows clear signatures for the presence of a bright blob south of the star and several photospheric radii away. The flux ratio between blob and star is between 5% and 10%. This blob also shows up as an additional absorption component in the red wings of the CO lines.

  6. Reduced subcommissural organ glycoprotein immunoreactivity precedes aqueduct closure and ventricular dilatation in H-Tx rat hydrocephalus.

    PubMed

    Somera, K C; Jones, H C

    2004-03-01

    The H-Tx rat has fetal-onset hydrocephalus associated with closure of the cerebral aqueduct and a reduction in the secretory cells of the subcommissural organ (SCO), a circumventricular organ situated in the dorsal wall of the cerebral aqueduct. The objective of this study was to determine the role of the SCO in hydrocephalus pathogenesis. Serial brain sections through aqueduct regions containing the SCO from H-Tx rats, together with non-hydrocephalic Fischer F344 rats, were studied at E16, before hydrocephalus onset, at E17, the beginning of onset, and at P0 when the hydrocephalus was overt. Tissues were immunostained by AFRU, an antibody against the SCO glycoprotein, and for the intermediate filament nestin. The area of SCO cells with AFRU immunostaining and the severity of lateral ventricle dilatation were quantified by image analysis. At E16 all fetuses had distinct SCO ependymal cells, open aqueducts and normal lateral ventricles. The H-Tx fetuses fell into two groups with large areas and small areas of AFRU immunoreactivity, all with a full complement of SCO cells. By E17, fetuses with small areas of immunoreactivity had reduced numbers of tall SCO secretory cells, and most had aqueducts closed posteriorly and dilated ventricles. Three additional fetuses with small areas of immunoreactivity had narrow but patent aqueducts and normal ventricles, and another had an open aqueduct and dilated ventricles. At P0, pups previously identified as hydrocephalic had small areas of AFRU immunoreactivity, an aqueduct that was closed anteriorly but open posteriorly, ventricular dilatation, and an absence of SCO secretory cells. The aqueduct even when closed was lined by typical ependymal cells throughout. Decreased nestin immunostaining accompanied the SCO changes. It is concluded that reduced SCO glycoprotein immunoreactivity precedes both aqueduct closure and expansion of the lateral ventricles in the H-Tx rat.

  7. 75 FR 52924 - Designation for the Amarillo, TX; Cairo, IL; State of Louisiana; State of North Carolina; Belmond...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-30

    ... information regarding the designation criteria in section 7(f)(l) of the USGSA (7 U.S.C. 79(f)) and determined... Headquarters location and telephone start end Amarillo Amarillo, TX (806-372-2152)........ 10/1/2010 9/30/2013 Additional Location: Guymon, OK.... Cairo Cairo, IL (618-734-1316) 10/1/2010 9/30/2013 Louisiana Baton...

  8. 77 FR 48960 - Foreign-Trade Zone 12-McAllen, TX Notification of Proposed Export Production Activity TST NA Trim...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-15

    ... Activity TST NA Trim, LLC (Fabric/Leather Lamination and Cutting) Hidalgo, TX The McAllen Foreign Trade... TST NA Trim, LLC (TST), located in Hidalgo, Texas. The notification conforming to the requirements...

  9. 76 FR 68188 - Valero Refining-Texas, L.P. v. Port of Corpus Christi Authority of Nueces County, TX; Notice of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-03

    ... of Corpus Christi Authority of Nueces County, TX; Notice of Filing of Complaint and Assignment Notice... Refining-Texas, L.P., hereinafter ``Complainant,'' against the Port of Corpus Christi Authority of...

  10. Structural studies of 1-butyl-3-methylimidazolium tetrafluoroborate/TX-100/ p-xylene ionic liquid microemulsions.

    PubMed

    Gao, Yan'an; Zhang, Jin; Xu, Hongyan; Zhao, Xueyan; Zheng, Liqiang; Li, Xinwei; Yu, Li

    2006-07-17

    The ionic liquid (IL) 1-butyl-3-methylimidazolium tetrafluoroborate (bmimBF4) forms nonaqueous microemulsions with p-xylene, with the aid of the nonionic surfactant TX-100. The phase behavior of the ternary system is investigated, and three microregions of the microemulsions-ionic liquid-in-oil (IL/O), bicontinuous, and oil-in-ionic liquid (O/IL)-are identified by conductivity measurements, according to percolation theory. On the basis of a phase diagram, a series of IL/O microemulsions are chosen and characterized by dynamic light scattering (DLS). The size of aggregates increases on increasing the amount of added polar component (bmimBF(4)), which is a similar phenomenon to that observed for typical water-in-oil (W/O) microemulsions, suggesting the formation of IL/O microemulsions. The microstructural characteristics of the microemulsions are investigated by FTIR and 1H NMR spectroscopy. The results indicate that the interaction between the electronegative oxygen atoms of the oxyethylene (OE) units in TX-100 and the electropositive imidazolium ring may be the driving force for the solubilization of bmimBF4 into the core of the TX-100 aggregates. In addition, the micropolarity of the microemulsions is investigated by using methyl orange (MO) as a UV/Vis spectroscopic probe. A relatively constant polarity of the microemulsion droplets is obtained in the IL microemulsion. Finally, a plausible structure for the IL/O microemulsion is presented.

  11. Electrochemical and optical study of carotenoids in TX 100 micelles: Electron transfer and a large blue shift

    SciTech Connect

    He, Z.; Kispert, L.D.

    1999-10-21

    The first oxidation waves of 8{prime}-apo-{beta}-caroten-8{prime}-al (I) and 8{prime}-apo-{beta}-caroten-8{prime}nitrile (II) in TX100 micelles are clearly observed in their cyclic voltammograms (CVs). The CV of {beta}-carotene (III) in TX100 micelles shows that III is not oxidized. It is proposed that the hydrophobic barrier of the micelle is an important reason for the failure to oxidize III, which is totally located in the hydrophobic center of the micelle. The oxidation of I and II demonstrates that electrons can be transferred through the terminal groups over a distance of ca. 22 {angstrom}. An unusually large blue band shift (100 nm, relative to that in CH{sub 2}Cl{sub 2}) is observed in the optical absorption spectrum of 7{prime}-apo-7{prime},7{prime}-dicyano-{beta}-carotene (IV) in TX100 micelles. This phenomenon is not observed in the absorption spectra of other studied carotenoids. A change in the ground-state electronic structure of IV, due to the influence of water near the terminal dicyanomethylidene group, is proposed to be the major reason for this large band shift.

  12. A potent and Kv1.3-selective analogue of the scorpion toxin HsTX1 as a potential therapeutic for autoimmune diseases

    NASA Astrophysics Data System (ADS)

    Rashid, M. Harunur; Huq, Redwan; Tanner, Mark R.; Chhabra, Sandeep; Khoo, Keith K.; Estrada, Rosendo; Dhawan, Vikas; Chauhan, Satendra; Pennington, Michael W.; Beeton, Christine; Kuyucak, Serdar; Norton, Raymond S.

    2014-03-01

    HsTX1 toxin, from the scorpion Heterometrus spinnifer, is a 34-residue, C-terminally amidated peptide cross-linked by four disulfide bridges. Here we describe new HsTX1 analogues with an Ala, Phe, Val or Abu substitution at position 14. Complexes of HsTX1 with the voltage-gated potassium channels Kv1.3 and Kv1.1 were created using docking and molecular dynamics simulations, then umbrella sampling simulations were performed to construct the potential of mean force (PMF) of the ligand and calculate the corresponding binding free energy for the most stable configuration. The PMF method predicted that the R14A mutation in HsTX1 would yield a > 2 kcal/mol gain for the Kv1.3/Kv1.1 selectivity free energy relative to the wild-type peptide. Functional assays confirmed the predicted selectivity gain for HsTX1[R14A] and HsTX1[R14Abu], with an affinity for Kv1.3 in the low picomolar range and a selectivity of more than 2,000-fold for Kv1.3 over Kv1.1. This remarkable potency and selectivity for Kv1.3, which is significantly up-regulated in activated effector memory cells in humans, suggest that these analogues represent valuable leads in the development of therapeutics for autoimmune diseases.

  13. Clinical commissioning and use of the Novalis Tx linear accelerator for SRS and SBRT.

    PubMed

    Kim, Jinkoo; Wen, Ning; Jin, Jian-Yue; Walls, Nicole; Kim, Sangroh; Li, Haisen; Ren, Lei; Huang, Yimei; Doemer, Anthony; Faber, Kathleen; Kunkel, Tina; Balawi, Ahssan; Garbarino, Kimberly; Levin, Kenneth; Patel, Samir; Ajlouni, Munther; Miller, Brett; Nurushev, Teamor; Huntzinger, Calvin; Schulz, Raymond; Chetty, Indrin J; Movsas, Benjamin; Ryu, Samuel

    2012-05-10

    The purpose of this study was to perform comprehensive measurements and testing of a Novalis Tx linear accelerator, and to develop technical guidelines for com-missioning from the time of acceptance testing to the first clinical treatment. The Novalis Tx (NTX) linear accelerator is equipped with, among other features, a high-definition MLC (HD120 MLC) with 2.5 mm central leaves, a 6D robotic couch, an optical guidance positioning system, as well as X-ray-based image guidance tools to provide high accuracy radiation delivery for stereotactic radiosurgery and stereotactic body radiation therapy procedures. We have performed extensive tests for each of the components, and analyzed the clinical data collected in our clinic. We present technical guidelines in this report focusing on methods for: (1) efficient and accurate beam data collection for commissioning treatment planning systems, including small field output measurements conducted using a wide range of detectors; (2) commissioning tests for the HD120 MLC; (3) data collection for the baseline characteristics of the on-board imager (OBI) and ExacTrac X-ray (ETX) image guidance systems in conjunction with the 6D robotic couch; and (4) end-to-end testing of the entire clinical process. Established from our clinical experience thus far, recommendations are provided for accurate and efficient use of the OBI and ETX localization systems for intra- and extracranial treatment sites. Four results are presented. (1) Basic beam data measurements: Our measurements confirmed the necessity of using small detectors for small fields. Total scatter factors varied significantly (30% to approximately 62%) for small field measurements among detectors. Unshielded stereotactic field diode (SFD) overestimated dose by ~ 2% for large field sizes. Ion chambers with active diameters of 6 mm suffered from significant volume averaging. The sharpest profile penumbra was observed for the SFD because of its small active diameter (0.6 mm). (2

  14. The relationship between seismicity and wastewater injection in Johnson County, TX

    NASA Astrophysics Data System (ADS)

    Lee, S. S.; Walter, J. I.; Frohlich, C.; DeShon, H. R.

    2015-12-01

    In light of recent research that suggests some high-rate wastewater injection wells from commercial oil and gas operations are casually linked to recent earthquakes in North Texas and Oklahoma, we apply a waveform cross-correlation technique to continuous waveform data from 38 seismic stations across Texas and Oklahoma using templates from 96 cataloged events from 2010-2015. We focus on an area near Venus TX, 40 km south of the Dallas-Fort Worth metropolitan area in Johnson County where a M4.0 earthquake was reported by the USGS on 7 May 2015. The epicenter was within several km of 4 high rate wells with max injection rates ranging between 684,000-833,000 barrels/month. Templates were chosen from the USGS and locally derived Venus aftershock sequence, events in the ANSS catalog with a minimum magnitude of 2 between 2009-2015, and previously located earthquakes recorded by USArray stations between 2009-2011. In Johnson County, there are 27 wastewater injection wells, each with a reported maximum injection rate of 104,000 barrels/month and greater. We detected 494 events within Johnson and adjacent counties, with approximately 36% of the detections occurring within 10 km of the Venus earthquake, spanning March 2010 to June 2015. Most of the seismicity occurs adjacent to higher rate injection wells, suggesting a link between monthly injection rate and earthquake occurrence. Template matching allows us to detect earthquakes otherwise too small to be picked up by routine earthquake identification methods and informs our research concerning the presence and spatial distribution of possibly induced, small magnitude earthquakes. We plan to apply this technique to detect seismicity that may have occurred prior to the start of wastewater injection or felt earthquakes.

  15. Fate and distribution of brevetoxin (PbTx) following lysis of Karenia brevis by algicidal bacteria, including analysis of open A-ring derivatives.

    PubMed

    Roth, Patricia B; Twiner, Michael J; Wang, Zhihong; Bottein Dechraoui, Marie-Yasmine; Doucette, Gregory J

    2007-12-15

    Flavobacteriaceae (strain S03) and Cytophaga sp. (strain 41-DBG2) are algicidal bacteria active against the brevetoxin (PbTx)-producing, red tide dinoflagellate, Karenia brevis. Little is known about the fate of PbTx associated with K. brevis cells following attack by such bacteria. The fate and distribution of PbTx in K. brevis cultures exposed to these algicidal strains were thus examined by receptor binding assay and liquid chromatography/mass spectrometry (LC/MS) in three size fractions (>5, 0.22-5, <0.22microm) over a 2-week time course. In control cultures, brevetoxin concentrations in the >5microm particulate size fraction correlated with changes in cell density, whereas significant increases in dissolved (i.e., <0.22microm) toxin were observed in the later stages of culture growth. Exposure of K. brevis to either of the two algicidal bacteria tested caused cell lysis, coinciding with a rapid decline in the >5microm PbTX size fraction and a simultaneous release of dissolved toxin into the growth medium. Upon cell lysis, dissolved brevetoxin accounted for ca. 60% of total toxin and consisted of 51-82% open A-ring derivatives. Open A-ring PbTx-2 and PbTx-3 derivatives bound with lower affinity (approximately 22- and 57-fold, respectively) to voltage-gated sodium channels and were considerably less cytotoxic (86- and 142-fold, respectively) to N2A cells than their individual parent toxins (i.e., PbTx-2 and PbTx-3). These novel findings of changes in PbTx size-fractioned distribution and overall reduction in K. brevis toxicity following attack by algicidal bacteria improve our understanding of potential trophic transfer routes and the fate of PbTx during red tide events. Moreover, this information will be important to consider when evaluating the potential role of algicidal bacteria in harmful algal bloom (HAB) management strategies involving control of bloom populations.

  16. Solution structure of GxTX-1E, a high affinity tarantula toxin interacting with voltage sensors in Kv2.1 potassium channels†

    PubMed Central

    Lee, Seungkyu; Milescu, Mirela; Jung, Hyun Ho; Lee, Ju Yeon; Bae, Chan Hyung; Lee, Chul Won; Kim, Ha Hyung; Swartz, Kenton J.; Kim, Jae Il

    2010-01-01

    GxTX-1E is a neurotoxin recently isolated from Plesiophrictus guangxiensis venom that inhibits the Kv2.1 channel in pancreatic β-cells. The sequence of the toxin is related to previously studied tarantula toxins that interact with the voltage sensors in Kv channels, and GxTX-1E interacts with the Kv2.1 channel with unusually high affinity, making it particularly useful for structural and mechanistic studies. Here we determined the three-dimensional solution structure of GxTX-1E using NMR spectroscopy and compared it to that of several related tarantula toxins. The molecular structure of GxTX-1E is similar to tarantula toxins that target voltage sensors in Kv channels in that it contains an ICK motif, composed of β-strands, and contains a prominent cluster of solvent-exposed hydrophobic residues surrounded by polar residues. When compared with the structure of SGTx1, a toxin for which mutagenesis data are available, the residue compositions of the two toxins are distinct in regions that are critical for activity, suggesting that their modes of binding to voltage sensors may be different. Interestingly, the structural architecture of GxTX-1E is also similar to JZTX-III, a tarantula toxin that interacts with Kv2.1 with low affinity. The most striking structural differences between GxTX-1E and JZTX-III are found in the orientation between the first and second cysteine loops and the C-terminal region of the toxins, suggesting that these regions of GxTX-1E are responsible for its high affinity. PMID:20509680

  17. Tx/Rx Head Coil Induces Less RF Transmit-Related Heating than Body Coil in Conductive Metallic Objects Outside the Active Area of the Head Coil

    PubMed Central

    Nagy, Zoltan; Oliver-Taylor, Aaron; Kuehne, Andre; Goluch, Sigrun; Weiskopf, Nikolaus

    2017-01-01

    The transmit–receive (Tx/Rx) birdcage head coil is often used for excitation instead of the body coil because of the presumably lower risk of heating in and around conductive implants. However, this common practice has not been systematically tested. To investigate whether the Tx/Rx birdcage head coil produces less heating than the body coil when scanning individuals with implants, we used a 3T clinical scanner and made temperature measurements around a straight 15 cm conductor using either the Tx/Rx body or the head coil for excitation. Additionally, the transmitted fields of a Tx/Rx head coil were measured both in air and in gel using a resonant and a non-resonant B field probes as well as a non-resonant E field probe. Simulations using a finite-difference time domain solver were compared with the experimental findings. When the body coil was used for excitation, we observed heating around the 15 cm wire at various anatomical locations (both within and outside of the active volume of the head coil). Outside its active area, no such heating was observed while using the Tx/Rx head coil for excitation. The E and B fields of the Tx/Rx birdcage head coil extended well-beyond the physical dimensions of the coil. In air, the fields were monotonically decreasing, while in gel they were more complex with local maxima at the end of the ASTM phantom. These experimental findings were line with the simulations. While caution must always be exercised when scanning individuals with metallic implants, these findings support the use of the Tx/Rx birdcage head coil in place of the body coil at 3T in order to reduce the risk of heating in and around conductive implants that are remote from the head coil. PMID:28184184

  18. Tx/Rx Head Coil Induces Less RF Transmit-Related Heating than Body Coil in Conductive Metallic Objects Outside the Active Area of the Head Coil.

    PubMed

    Nagy, Zoltan; Oliver-Taylor, Aaron; Kuehne, Andre; Goluch, Sigrun; Weiskopf, Nikolaus

    2017-01-01

    The transmit-receive (Tx/Rx) birdcage head coil is often used for excitation instead of the body coil because of the presumably lower risk of heating in and around conductive implants. However, this common practice has not been systematically tested. To investigate whether the Tx/Rx birdcage head coil produces less heating than the body coil when scanning individuals with implants, we used a 3T clinical scanner and made temperature measurements around a straight 15 cm conductor using either the Tx/Rx body or the head coil for excitation. Additionally, the transmitted fields of a Tx/Rx head coil were measured both in air and in gel using a resonant and a non-resonant B field probes as well as a non-resonant E field probe. Simulations using a finite-difference time domain solver were compared with the experimental findings. When the body coil was used for excitation, we observed heating around the 15 cm wire at various anatomical locations (both within and outside of the active volume of the head coil). Outside its active area, no such heating was observed while using the Tx/Rx head coil for excitation. The E and B fields of the Tx/Rx birdcage head coil extended well-beyond the physical dimensions of the coil. In air, the fields were monotonically decreasing, while in gel they were more complex with local maxima at the end of the ASTM phantom. These experimental findings were line with the simulations. While caution must always be exercised when scanning individuals with metallic implants, these findings support the use of the Tx/Rx birdcage head coil in place of the body coil at 3T in order to reduce the risk of heating in and around conductive implants that are remote from the head coil.

  19. Biochemical and functional studies of ColTx-I, a new myotoxic phospholipase A2 isolated from Crotalus oreganus lutosus (Great Basin rattlesnake) snake venom.

    PubMed

    Almeida, J R; Resende, L M; Silva, A G; Ribeiro, R I M A; Stábeli, R G; Soares, A M; Calderon, L A; Marangoni, S; Da Silva, S L

    2016-07-01

    Commonly, phospholipases A2 (PLA2s) play key roles in the pathogenesis of the local tissue damage characteristic of crotaline and viperine snake envenomations. Crotalus oreganus lutosus snake venom has not been extensively studied; therefore, the characterization of its components represents a valuable biotechnological tool for studying pathophysiological processes of envenoming and for gaining a deeper understanding of its biological effects. In this study, for the first time, a basic PLA2 myotoxin, ColTx-I, was purified from C. o. lutosus through two chromatographic steps. ColTx-I is monomeric with calculated molecular mass weight (Mw) of 14,145 Da and a primary structure closely related to basic PLA2s from viperid venoms. The pure enzyme has a specific activity of 15.87 ± 0.65 nmol/min/mg at optimal conditions (pH 8.0 and 37 °C). ColTx-I activity was found to be dependent on Ca(2+), as its substitution by other ionic species as well as the addition of chelating agents significantly reduced its phospholipase activity. In vivo, ColTx-I triggered dose-dependent inflammatory responses, measured using the paw edema model, with an increase in IL-6 levels, systemic and local myotoxicity, characterized by elevated plasma creatine kinase activity. ColTx-I induced a complex series of degenerative events associated with edema, inflammatory infiltrate and skeletal muscle necrosis. These biochemical and functional results suggest that ColTx-I, a myotoxic and inflammatory mediator, plays a relevant role in C. o. lutosus envenomation. Thus, detailed studies on its mechanism of action, such as evaluating the synergism between ColTx-I and other venom components may reveal targets for the development of more specific and effective therapies.

  20. Inflammatory oedema induced by Lachesis muta muta (Surucucu) venom and LmTX-I in the rat paw and dorsal skin.

    PubMed

    Ferreira, Tatiane; Camargo, Enilton A; Ribela, Maria Teresa C P; Damico, Daniela C; Marangoni, Sérgio; Antunes, Edson; De Nucci, Gilberto; Landucci, Elen C T

    2009-01-01

    The ability of crude venom and a basic phospholipase A(2) (LmTX-I) from Lachesis muta muta venom to increase the microvascular permeability in rat paw and skin was investigated. Crude venom or LmTX-I were injected subplantarly or intradermally and rat paw oedema and dorsal skin plasma extravasation were measured. Histamine release from rat peritoneal mast cell was also assessed. Crude venom or LmTX-I induced dose-dependent rat paw oedema and dorsal skin plasma extravasation. Venom-induced plasma extravasation was inhibited by the histamine H(1) antagonist mepyramine (6mg/kg), histamine/5-hydroxytriptamine antagonist cyproheptadine (2mg/kg), cyclooxygenase inhibitor indomethacin (5mg/kg), nitric oxide synthesis inhibitor l-NAME (100nmol/site), tachykinin NK(1) antagonist SR140333 (1nmol/site) and bradykinin B(2) receptor antagonist Icatibant (0.6mg/kg). Platelet-activating factor (PAF) antagonist PCA4248 (5mg/kg) had no effect. LmTX-I-induced skin extravasation was inhibited by cyproheptadine, mepyramine, indomethacin and PCA4248, while l-NAME and SR140333 had no effect. Additionally, both Lachesis muta muta venom and LmTX-I concentration-dependently induced histamine release from rat mast cells. In conclusion, Lachesis muta muta venom and LmTX-I increase microvascular permeability by mechanisms involving in vivo mast cell activation and arachidonic acid metabolites. Additionally, crude venom-induced responses also involve substance P, nitric oxide and bradykinin release, whether LmTX-I-induced responses involve PAF.

  1. Structural and functional consequences of the presence of a fourth disulfide bridge in the scorpion short toxins: solution structure of the potassium channel inhibitor HsTX1.

    PubMed Central

    Savarin, P.; Romi-Lebrun, R.; Zinn-Justin, S.; Lebrun, B.; Nakajima, T.; Gilquin, B.; Menez, A.

    1999-01-01

    We have determined the three-dimensional structure of the potassium channel inhibitor HsTX1, using nuclear magnetic resonance and molecular modeling. This protein belongs to the scorpion short toxin family, which essentially contains potassium channel blockers of 29 to 39 amino acids and three disulfide bridges. It is highly active on voltage-gated Kv1.3 potassium channels. Furthermore, it has the particularity to possess a fourth disulfide bridge. We show that HsTX1 has a fold similar to that of the three-disulfide-bridged toxins and conserves the hydrophobic core found in the scorpion short toxins. Thus, the fourth bridge has no influence on the global conformation of HsTX1. Most residues spatially analogous to those interacting with voltage-gated potassium channels in the three-disulfide-bridged toxins are conserved in HsTX1. Thus, we propose that Tyr21, Lys23, Met25, and Asn26 are involved in the biological activity of HsTX1. As an additional positively charged residue is always spatially close to the aromatic residue in toxins blocking the voltage-gated potassium channels, and as previous mutagenesis experiments have shown the critical role played by the C-terminus in HsTX1, we suggest that Arg33 is also important for the activity of the four disulfide-bridged toxin. Docking calculations confirm that, if Lys23 and Met25 interact with the GYGDMH motif of Kv1.3, Arg33 can contact Asp386 and, thus, play the role of the additional positively charged residue of the toxin functional site. This original configuration of the binding site of HsTX1 for Kv1.3, if confirmed experimentally, offers new structural possibilities for the construction of a molecule blocking the voltage-gated potassium channels. PMID:10631983

  2. Biochemical Characterization and Pharmacological Properties of New Basic PLA2 BrTX-I Isolated from Bothrops roedingeri (Roedinger's Lancehead) Mertens, 1942, Snake Venom

    PubMed Central

    Gomes Heleno, Mauricio Aurelio; Baldasso, Paulo Aparecido; Ponce-Soto, Luis Alberto; Marangoni, Sérgio

    2013-01-01

    BrTX-I, a PLA2, was purified from Bothrops roedingeri venom after only one chromatographic step using reverse-phase HPLC on μ-Bondapak C-18 column. A molecular mass of 14358.69 Da was determined by MALDI-TOF mass spectrometry. Amino acid analysis showed a high content of hydrophobic and basic amino acids as well as 14 half-cysteine residues. The total amino acid sequence was obtained using SwissProt database and showed high amino acid sequence identity with other PLA2 from snake venom. The amino acid composition showed that BrTX-I has a high content of Lys, Tyr, Gly, Pro, and 14 half-Cys residues, typical of a basic PLA2. BrTX-I presented PLA2 activity and showed a minimum sigmoidal behavior, reaching its maximal activity at pH 8.0, 35–45°C, and required Ca2+. In vitro, the whole venom and BrTX-I caused a neuromuscular blockade in biventer cervicis preparations in a similar way to other Bothrops species. BrTX-I induced myonecrosis and oedema-forming activity analyzed through injection of the purified BrTX-I in mice. Since BrTX-I exerts a strong proinflammatory effect, the enzymatic phospholipid hydrolysis might be relevant for these phenomena; incrementing levels of IL-1, IL-6, and TNFα were observed at 15 min, 30 min, one, two, and six hours postinjection, respectively. PMID:23509747

  3. Isolation and characterization of SsmTx-I, a Specific Kv2.1 blocker from the venom of the centipede Scolopendra Subspinipes Mutilans L. Koch.

    PubMed

    Chen, Minzhi; Li, Jing; Zhang, Fan; Liu, Zhonghua

    2014-03-01

    Scolopendra subspinipes mutilans, also known as Chinese red-headed centipede, is a venomous centipede from East Asia and Australasia. Venom from this animal has not been researched as thoroughly as venom from snakes, snails, scorpions, and spiders. In this study, we isolated and characterized SsmTx-I, a novel neurotoxin from the venom of S. subspinipes mutilans. SsmTx-I contains 36 residues with four cysteines forming two disulfide bonds. It had low sequence similarity (<10%) with other identified peptide toxins. By whole-cell recording, SsmTx-I significantly blocked voltage-gated K⁺ channels in dorsal root ganglion neurons with an IC₅₀ value of 200 nM, but it had no effect on voltage-gated Na⁺ channels. Among the nine K⁺ channel subtypes expressed in human embryonic kidney 293 cells, SsmTx-I selectively blocked the Kv2.1 current with an IC₅₀ value of 41.7 nM, but it had little effect on currents mediated by other K⁺ channel subtypes. Blockage of Kv2.1 by SsmTx-I was not associated with significant alteration of steady-state activation, suggesting that SsmTx-I might act as a simple inhibitor or channel blocker rather than a gating modifier. Our study reported a specific Kv2.1-blocker from centipede venom and provided a basis for future investigations of SsmTx-I, for example on structure-function relationships, mechanism of action, and pharmacological potential.

  4. Geophysical Investigation of an Abandoned Cemetery: Teachers Discover Evidence of Unmarked Graves in Prairie View, TX

    NASA Astrophysics Data System (ADS)

    Henning, A. T.; Sawyer, D. S.; Baldwin, R.; Kahera, A.; Thoms, A.

    2007-12-01

    In July 2007, a group of nineteen K-12 teachers investigated an abandoned cemetery in Prairie View, Texas, utilizing ground-penetrating radar (GPR) to image the subsurface. In a period of two weeks, the group acquired and interpreted 59 GPR profiles in Wyatt Chapel Cemetery and surrounding areas in order to determine the local stratigraphy and try to locate unmarked graves. The sandy soil in this area is ideally suited for GPR investigations and numerous geophysical anomalies were identified. Wyatt Chapel Cemetery is located adjacent to the campus of Prairie View A&M University in Prairie View, TX, and is thought to have originated as a slave burial ground in the 1850's. Participants in a summer course at Rice University conducted a geophysical investigation of the site. Participants were in-service K-12 teachers from urban Houston school districts where the majority of students are members of historically underrepresented minority groups. Recruitment efforts targeted educators who are currently teaching science without a science degree. Participants included elementary, middle and high school teachers. This summer experience is followed by a content-intensive academic year course in Physical Geology. GPR is an excellent tool for investigating the sandy soil encountered at Wyatt Chapel Cemetery. The stratigraphy in the area consists of 3-6 feet of reddish-brown, medium-grained sand overlying a light gray, highly compacted clay. The sand-clay boundary appears as a strong reflector on the GPR profiles. Participants identified numerous anomalies in the GPR data and two were excavated. One consisted of a pair of bright hyperbolae, suggesting two edges of a metal object. This excavation resulted in the discovery of a metal plank thought to be a burial cover. The second anomaly consisted of a break in the horizon representing the top of the clay layer, and subsequent excavation revealed a grave shaft. Participants experienced the process of science first-hand and used

  5. A Large-N Mixed Sensor Active + Passive Seismic Array near Sweetwater, TX

    NASA Astrophysics Data System (ADS)

    Barklage, M.; Hollis, D.; Gridley, J. M.; Woodward, R.; Spriggs, N.

    2014-12-01

    A collaborative high-density seismic survey using broadband and short period seismic sensors was conducted March 7 - April 30, 2014 near Sweetwater, TX. The objective of the survey was to use a combination of controlled source shot slices and passive seismic recordings recorded by multiple types of sensors with different bandwidths and sensitivities to image the subsurface. The broadband component of the survey consisted of 25 continuously recording seismic stations comprised of 20 Trillium Compact Posthole sensors from Nanometrics and 5 Polar Trillium 120PHQs from the IRIS/PASSCAL Instrument Center (PIC). The broadband stations also utilized 25 Centaur digitizers from Nanometrics as well as 25 polar quick deploy enclosures from the PIC. The broadband array was designed to maximize horizontal traveling seismic energy for surface wave analysis over the primary target area with sufficient offset for imaging objectives at depth. The short period component of the survey consisted of 2639 receiver locations using Zland nodes from NodalSeismic. The nodes are further divided into 3 sub-arrays: 1) outlier array 2) active source array 3) backbone array. The outlier array consisted of 25 continuously recording nodes distributed around the edge of the survey at a distance of ~5 km from the survey boundary, and provided valuable constraints to passive data analysis techniques at the edge of the survey boundary. The active source patch consisted of densely spaced nodes that were designed to record signals from a Vibroseis source truck for active source reflection processing and imaging. The backbone array consisted of 292 nodes that covered the entirety of the survey area to maximize the value of the passive data analysis. By utilizing continuous recording and smartly designed arrays for measuring local and regional earthquakes we can incorporate velocity information derived from passive data analysis into the active source processing workflow to produce a superior subsurface

  6. Imaging system QA of a medical accelerator, Novalis Tx, for IGRT per TG 142: our 1 year experience.

    PubMed

    Chang, Zheng; Bowsher, James; Cai, Jing; Yoo, Sua; Wang, Zhiheng; Adamson, Justus; Ren, Lei; Yin, Fang-Fang

    2012-07-05

    American Association of Physicists in Medicine (AAPM) task group (TG) 142 has recently published a report to update recommendations of the AAPM TG 40 report and add new recommendations concerning medical accelerators in the era of image-guided radiation therapy (IGRT). The recommendations of AAPM TG 142 on IGRT are timely. In our institute, we established a comprehensive imaging QA program on a medical accelerator based on AAPM TG 142 and implemented it successfully. In this paper, we share our one-year experience and performance evaluation of an OBI capable linear accelerator, Novalis Tx, per TG 142 guidelines.

  7. Occurrence of metallothionein gene smtA in synechococcus Tx-20 and other blue-green algae

    SciTech Connect

    Robinson, N.J.; Gupta, A.; Huckle, J.W.; Jackson, P.; Whitton, B.A. )

    1990-06-01

    Blue-green algae are often abundant at Zn- and Cd-contaminated sites. In order to understand the mechanisms associated with Zn- and Cd-tolerance, we have isolated a metallothionein gene, designated smtA, in Synechococcus Tx-20 (- Pcc 6301 - Anacystis nidulans), a strain apparently obtained from an unpolluted site. The gene was cloned and sequenced, and its expression investigated in a range of heavy-metal-tolerant strains of the same organism obtained by stepwise adaptation. The polymerase chain reaction was used to probe for the possible presence of the homologous gene in a range of other strains (especially Synechococcus) isolated from sites without and with heavy metal contamination.

  8. Sustained inhibition of the NaV1.7 sodium channel by engineered dimers of the domain II binding peptide GpTx-1.

    PubMed

    Murray, Justin K; Biswas, Kaustav; Holder, J Ryan; Zou, Anruo; Ligutti, Joseph; Liu, Dong; Poppe, Leszek; Andrews, Kristin L; Lin, Fen-Fen; Meng, Shi-Yuan; Moyer, Bryan D; McDonough, Stefan I; Miranda, Les P

    2015-11-01

    Many efforts are underway to develop selective inhibitors of the voltage-gated sodium channel NaV1.7 as new analgesics. Thus far, however, in vitro selectivity has proved difficult for small molecules, and peptides generally lack appropriate pharmacokinetic properties. We previously identified the NaV1.7 inhibitory peptide GpTx-1 from tarantula venom and optimized its potency and selectivity via structure-guided analoging. To further understand GpTx-1 binding to NaV1.7, we have mapped the binding site to transmembrane segments 1-4 of the second pseudosubunit internal repeat (commonly referred to as Site 4) using NaV1.5/NaV1.7 chimeric protein constructs. We also report that select GpTx-1 amino acid residues apparently not contacting NaV1.7 can be derivatized with a hydrophilic polymer without adversely affecting peptide potency. Homodimerization of GpTx-1 with a bifunctional polyethylene glycol (PEG) linker resulted in a compound with increased potency and a significantly reduced off-rate, demonstrating the ability to modulate the function and properties of GpTx-1 by linking to additional molecules.

  9. Characterization of a Novel Alpha-Conotoxin TxID from Conus textile that Potently Blocks rat Alpha3beta4 Nicotinic Acetylcholine Receptors

    PubMed Central

    Luo, Sulan; Zhangsun, Dongting; Zhu, Xiaopeng; Wu, Yong; Hu, Yuanyan; Christensen, Sean; Harvey, Peta J.; Akcan, Muharrem; Craik, David J.; McIntosh, J. Michael

    2014-01-01

    The α3β4 nAChRs are implicated in pain sensation in the PNS and addiction to nicotine in the CNS. We identified an α-4/6-conotoxin (CTx) TxID from Conus textile. The new toxin consists of 15 amino acid residues with two disulfide bonds. TxID was synthesized using solid phase methods and the synthetic peptide was functionally tested on nAChRs heterologously expressed in Xenopus laevis oocytes. TxID blocked rat α3β4 nAChRs with a 12.5 nM IC50, which places it amongst the most potent α3β4 nAChR antagonists. TxID also blocked the closely related α6/α3β4 with a 94 nM IC50 but showed little activity on other nAChR subtypes. NMR analysis showed that two major structural isomers exist in solution, one of which adopts a regular α-CTx fold but with different surface charge distribution to other 4/6 family members. α-CTx TxID is a novel tool with which to probe the structure and function of α3β4 nAChRs. PMID:24200193

  10. Growth of Pseudomonas sp. TX1 on a wide range of octylphenol polyethoxylate concentrations and the formation of dicarboxylated metabolites.

    PubMed

    Lin, Yi-Wen; Guo, Gia-Luen; Hsieh, Hsiao-Cheng; Huang, Shir-Ly

    2010-04-01

    Pseudomonas sp. TX1, is able to use octylphenol polyethoxylates (OPEO(n), or Triton X-100; average n = 9.5) as a sole carbon source. It can grow on 0.05-20% of OPEO(n) with a specific growth rate of 0.34-0.44 h(-1). High-performance liquid chromatography-mass spectrometer analysis of OPEO(n) degraded metabolites revealed that strain TX1 was able to shorten the ethoxylate chain and produce octylphenol (OP). Furthermore, formation of the short carboxylate metabolites, such as carboxyoctylphenol polyethoxylates (COPEO(n), n = 2, 3) and carboxyoctylphenol polyethoxycarboxylates (COPEC(n), n = 2, 3) began at the log stage, while octylphenol polyethoxycarboxylates (OPEC(n), n = 1-3) was formed at the stationary phase. All the short-ethoxylated metabolites, OPEO(n), OPEC(n), COPEO(n), and COPEC(n), accumulated when the cells were in the stationary phase. This study is the first to demonstrate the formation of COPEO(n) and COPEC(n) from OPEO(n) by an aerobic bacterium.

  11. Solution structure of BmKalphaTx11, a toxin from the venom of the Chinese scorpion Buthus martensii Karsch.

    PubMed

    Zhu, Jing; Tong, Xiaotian; Cao, Chunyang; Wu, Gong; Zhang, Naixia; Wu, Houming

    2010-01-01

    The solution structure of BmKalphaTx11 presented by this paper is distinctive from any other structures of wide-type scorpion alpha-toxins reported so far, for its trans-9,10 peptide bond conformation is accompanied by 'protruding' topology of the 'NC-domain'. The orientation of the C-tail of BmKalphaTx11 is obviously different from that of classical alpha-toxins (e.g., AaH2, BmK-M8), despite the fact that they share common trans conformation of peptide bond between residues 9 and 10. Accordingly, there must be other structural factors dominating the orientation of the C-tail except the conformation of peptide bond 9-10. Our study reveals that residues at position 58 play an important role in it, and different type of residues at this position (e.g., Lys, Arg, Met, Ile) result in different spatial relationship between the C-terminus and the 'five-residue-turn' and then different topology of the 'NC-domain', therefore residues at position 58 are believed to function as structure and bioactivity switch for specificity of scorpion alpha-toxins. The mechanism for stabilizing the geometry of the 'NC-domain' in wide-type scorpion alpha-toxins is also discussed.

  12. CoaTx-II, a new dimeric Lys49 phospholipase A2 from Crotalus oreganus abyssus snake venom with bactericidal potential: Insights into its structure and biological roles.

    PubMed

    Almeida, J R; Lancellotti, M; Soares, A M; Calderon, L A; Ramírez, D; González, W; Marangoni, S; Da Silva, S L

    2016-09-15

    Snake venoms are rich and intriguing sources of biologically-active molecules that act on target cells, modulating a diversity of physiological functions and presenting promising pharmacological applications. Lys49 phospholipase A2 is one of the multifunctional proteins present in these complex secretions and, although catalytically inactive, has a variety of biological activities, including cytotoxic, antibacterial, inflammatory, antifungal activities. Herein, a Lys49 phospholipase A2, denominated CoaTx-II from Crotalus oreganus abyssus, was purified and structurally and pharmacologically characterized. CoaTx-II was isolated with a high degree of purity by a combination of two chromatographic steps; molecular exclusion and reversed-phase high performance liquid chromatography. This toxin is dimeric with a mass of 13868.2 Da (monomeric form), as determined by mass spectrometry. CoaTx-II is rich in Arg and Lys residues and displays high identity with other Lys49 PLA2 homologues, which have high isoelectric points. The structural model of dimeric CoaTx-II shows that the toxin is non-covalently stabilized. Despite its enzymatic inactivity, in vivo CoaTx-II caused local muscular damage, characterized by increased plasma creatine kinase and confirmed by histological alterations, in addition to an inflammatory activity, as demonstrated by mice paw edema induction and pro-inflammatory cytokine IL-6 elevation. CoaTx-II also presents antibacterial activity against gram negative (Pseudomonas aeruginosa 31NM, Escherichia coli ATCC 25922) and positive (Staphyloccocus aureus BEC9393 and Rib1) bacteria. Therefore, data show that this newly purified toxin plays a central role in mediating the degenerative events associated with envenomation, in addition to demonstrating antibacterial properties, with potential for use in the development of strategies for antivenom therapy and combating antibiotic-resistant bacteria.

  13. SURFACE GEOPHYSICAL EXPLORATION OF TX-TY TANK FARMS AT THE HANFORD SITE RESULTS OF BACKGROUND CHARACTERIZATION WITH GROUND PENETRATING RADAR

    SciTech Connect

    MYERS DA; CUBBAGE R; BRAUCHLA R; O'BRIEN G

    2008-07-24

    Ground penetrating radar surveys of the TX and TY tank farms were performed to identify existing infrastructure in the near surface environment. These surveys were designed to provide background information supporting Surface-to-Surface and Well-to-Well resistivity surveys of Waste Management Area TX-TY. The objective of the preliminary investigation was to collect background characterization information with GPR to understand the spatial distribution of metallic objects that could potentially interfere with the results from high resolution resistivity{trademark} surveys. The results of the background characterization confirm the existence of documented infrastructure, as well as highlight locations of possible additional undocumented subsurface metallic objects.

  14. 2004 Initial Assessments for the T and TX TY Tank Farm Field Investigation Report (FIR): Numerical Simulations

    SciTech Connect

    Zhang, Z. F.; Freedman, Vicky L.; Waichler, Scott R.

    2004-09-24

    In support of CH2M HILL Hanford Group, Inc.’s (CHG) preparation of a Field Investigative Report (FIR) for the Hanford Site Single-Shell Tank Waste Management Area (WMA) T and TX-TY, a suite of numerical simulations of flow and solute transport was executed using the STOMP code to predict the performance of surface barriers for reducing long-term risks from potential groundwater contamination at the T and TX-TY WMA. The scope and parametric data for these simulations were defined by a modeling data package provided by CHG. This report documents the simulation involving 2-D cross sections through the T Tank and the TX-TY Tank Farm. Eight cases were carried out for the cross sections to simulate the effects of interim barrier, water line leak, inventory distribution, and surface recharge on water flow and the transport of long-lived radionuclides (i.e., technecium-99 and uranium) and chemicals (i.e., nitrate and chromium For simulations with barriers, it is assumed that an interim barrier is in place by the year 2010. It was also assumed that, for all simulations, as part of tank farm closure, a closure barrier was in place by the year 2040. The modeling considers the estimated inventories of contaminants within the vadose zone and calculates the associated risk. It assumes that no tanks will leak in the future. Initial conditions for contaminant concentration are provided as part of inventory estimates for uranium, technetium-99, nitrate, and chromium. For moisture flow modeling, Neumann boundary conditions are prescribed at the surface with the flux equal to the recharge rate estimate. For transport modeling, a zero flux boundary is prescribed at the surface for uranium, technetium-99, nitrate, and chromium. The western and eastern boundaries are assigned no-flux boundaries for both flow and transport. The water table boundary is prescribed by water table elevations and the unconfined aquifer hydraulic gradient. No-flux boundaries are used for the lower boundary

  15. Alternating magnetic anisotropy of Li2(Li1–xTx)N (T = Mn, Fe, Co, and Ni)

    DOE PAGES

    Jesche, A.; Ke, L.; Jacobs, J. L.; ...

    2015-05-11

    Substantial amounts of the transition metals Mn, Fe, Co, and Ni can be substituted for Li in single crystalline Li2(Li1–xTx)N. Isothermal and temperature-dependent magnetization measurements reveal local magnetic moments with magnitudes significantly exceeding the spin-only value. The additional contributions stem from unquenched orbital moments that lead to rare-earth-like behavior of the magnetic properties. Accordingly, extremely large magnetic anisotropies have been found. Most notably, the magnetic anisotropy alternates as easy plane→easy axis→easy plane→easy axis when progressing from T = Mn → Fe → Co → Ni. This behavior can be understood based on a perturbation approach in an analytical, single-ion model.more » As a result, the calculated magnetic anisotropies show surprisingly good agreement with the experiment and capture the basic features observed for the different transition metals.« less

  16. Arsenic Removal from Drinking Water by Adsorptive Media U.S. EPA Demonstration Project at Alvin, TX, Six-Month Evaluation Report

    EPA Science Inventory

    This report documents the activities performed and the results obtained from the first six months of the EPA arsenic removal technology demonstration project at the Oak Manor Municipal Utility District (MUD) facility at Alvin, TX. The main objective of the project is to evaluate...

  17. Registration of seed sterile, Perennial Sorghum spp. [Sorghum bicolor (L.) Moench x S. halepense (L.) Pers.] Hybrid 'PSH09TX15'

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A novel, Perennial Sorghum spp. Hybrid (PSH) [Sorghum bicolor (L.) Moench x S. halepense (L.) Pers.] ('PSH09TX15'; PI ______) was identified that possessed complete seed sterility, only rare occurrences (< 0.1%) of flowering, and significantly higher mean leaf number per tiller than S. halepense. I...

  18. Arsenic Removal from Drinking Water by Adsorptive Media U.S. EPA Demonstration Project at Wellman, TX, Final Performance Evaluation Report

    EPA Science Inventory

    This report documents the activities performed and the results obtained from the arsenic removal treatment technology demonstration project in the City of Wellman, TX. The main objective of the project was to evaluate the effectiveness of AdEdge Technologies’ AD-33 media in remo...

  19. Arsenic Removal from Drinking Water by Adsorptive Media. U.S. EPA Demonstration Project at Oak Manor Municipal Utility District at Alvin, TX. Final Performance Evaluation Report

    EPA Science Inventory

    This report documents the activities performed and the results obtained for the EPA arsenic removal technology demonstration project at the Oak Manor Municipal Utility District (MUD) facility in Alvin, TX. The objectives of the project were to evaluate 1) the effectiveness of a ...

  20. Antihyperalgesic effects of ProTx-II, a Nav1.7 antagonist, and A803467, a Nav1.8 antagonist, in diabetic mice.

    PubMed

    Tanaka, Ken-Ichiro; Sekino, Shota; Ikegami, Megumi; Ikeda, Hiroko; Kamei, Junzo

    2015-01-01

    The present study investigated the effects of intrathecal administration of ProTx-II (tarantula venom peptide) and A803467 (5-[4-chloro-phenyl]-furan-2-carboxylic acid [3,5-dimethoxy-phenyl]-amide), selective Nav1.7 and Nav1.8 antagonists, respectively, on thermal hyperalgesia in a painful diabetic neuropathy model of mice. Intrathecal administration of ProTx-II at doses from 0.04 to 4 ng to diabetic mice dose-dependently and significantly increased the tail-flick latency. Intrathecal administration of A803467 at doses from 10 to 100 ng to diabetic mice also dose-dependently and significantly increased the tail-flick latency. However, intrathecal administration of either ProTx-II (4 ng) or A803467 (100 ng) had no effect on the tail-flick latency in nondiabetic mice. The expression of either the Nav1.7 or Nav1.8 sodium channel protein in the dorsal root ganglion in diabetic mice was not different from that in nondiabetic mice. The present results suggest that ProTx-II and A803467, highly selective blockers of Nav1.7 and Nav1.8 sodium channels, respectively, in the spinal cord, can have antihyperalgesic effects in diabetic mice.

  1. Discovery and characterisation of a novel toxin from Dendroaspis angusticeps, named Tx7335, that activates the potassium channel KcsA

    PubMed Central

    Rivera-Torres, Iván O.; Jin, Tony B.; Cadene, Martine; Chait, Brian T.; Poget, Sébastien F.

    2016-01-01

    Due to their central role in essential physiological processes, potassium channels are common targets for animal toxins. These toxins in turn are of great value as tools for studying channel function and as lead compounds for drug development. Here, we used a direct toxin pull-down assay with immobilised KcsA potassium channel to isolate a novel KcsA-binding toxin (called Tx7335) from eastern green mamba snake (Dendroaspis angusticeps) venom. Sequencing of the toxin by Edman degradation and mass spectrometry revealed a 63 amino acid residue peptide with 4 disulphide bonds that belongs to the three-finger toxin family, but with a unique modification of its disulphide-bridge scaffold. The toxin induces a dose-dependent increase in both open probabilities and mean open times on KcsA in artificial bilayers. Thus, it unexpectedly behaves as a channel activator rather than an inhibitor. A charybdotoxin-sensitive mutant of KcsA exhibits similar susceptibility to Tx7335 as wild-type, indicating that the binding site for Tx7335 is distinct from that of canonical pore-blocker toxins. Based on the extracellular location of the toxin binding site (far away from the intracellular pH gate), we propose that Tx7335 increases potassium flow through KcsA by allosterically reducing inactivation of the channel. PMID:27044983

  2. Registration of high-oleic peanut germplasm line ARSOK-S1 (TX996784) with enhanced resistance to Sclerotinia blight and pod rot

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The high oleic Spanish peanut (Arachis hypogaea L. subsp. fastigiata var. vulgaris) germplasm line ARSOK-S1 was developed cooperatively between the USDA Agricultural Research Service, Texas AgriLife Research, and Oklahoma State University, and was released in 2013. ARSOK-S1 (tested early as TX99678...

  3. EPA Proposes to Add Burnet Co., TX, Groundwater Plume to National Priorities List of Superfund Sites, Two hazardous waste sites added, six proposed nationally

    EPA Pesticide Factsheets

    (DALLAS - March 24, 2015) The Environmental Protection Agency (EPA) proposed to add the Main Street Groundwater Plume site in Burnet Co., TX, to the National Priorities List (NPL) of Superfund sites, a list of sites that pose risks to people's healt

  4. EPA Adds Burnet Co., TX, Groundwater Plume to National Priorities List of Superfund Sites, Five hazardous waste sites added, seven proposed nationally

    EPA Pesticide Factsheets

    (DALLAS - Sept. 28, 2015) The Environmental Protection Agency (EPA) added the Main Street Groundwater Plume site in Burnet Co., TX, to the National Priorities List (NPL) of Superfund sites, a list of sites that pose risks to people's health and the

  5. Arsenic Removal from Drinking Water by Adsorptive Media U.S. EPA Demonstration Project at Wellman, TX, Six-Month Evaluation Report

    EPA Science Inventory

    This report documents the activities performed and the results obtained from the first six months of the arsenic removal treatment technology demonstration project in the City of Wellman, TX. The main objective of the project was to evaluate the effectiveness of AdEdge Technolog...

  6. ARSENIC REMOVAL FROM DRINKING WATER BY ADSORPTIVE MEDIA U.S. EPA DEMONSTRATION PROJECT AT BRUNI, TX, SIX-MONTH EVALUATION REPORT

    EPA Science Inventory

    This report documents the activities performed and the results obtained from the first six months of the arsenic removal treatment technology demonstration project at the Webb Consolidated Independent School District (Webb CISD) site at Bruni, TX. The main objective of the projec...

  7. Electrochemical and in-situ X-ray diffraction studies of Ti3C2Tx MXene in ionic liquid electrolyte

    DOE PAGES

    Lin, Zifeng; Rozier, Patrick; Duployer, Benjamin; ...

    2016-08-26

    2D titanium carbide (Ti3C2Tx MXene) showed good capacitance in both organic and neat ionic liquid electrolytes, but its charge storage mechanism is still not fully understood. Here, electrochemical characteristics of Ti3C2Tx electrode were studied in neat EMI-TFSI electrolyte. A capacitive behavior was observed within a large electrochemical potential range (from – 1.5 to 1.5 V vs. Ag). Intercalation and de-intercalation of EMI+ cations and/or TFSI– anions were investigated by in-situ X-ray diffraction. Interlayer spacing of Ti3C2Tx flakes decreases during positive polarization, which can be ascribed to either electrostatic attraction effect between intercalated TFSI– anions and positively charged Ti3C2Tx nanosheets ormore » steric effect caused by de-intercalation of EMI+ cations. In conclusion, the expansion of interlayer spacing when polarized to negative potentials is explained by steric effect of cation intercalation.« less

  8. 78 FR 63867 - Change of Using Agency for Restricted Areas R-5115, NM, and R-6316, R-6317, and R-6318, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-25

    ... Federal Aviation Administration 14 CFR Part 73 RIN 2120-AA66 Change of Using Agency for Restricted Areas R-5115, NM, and R- 6316, R-6317, and R-6318, TX AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action updates the name of the using agencies for Restricted Areas...

  9. Arsenic Removal from Drinking Water by Adsorptive Media U.S. EPA Demonstration Project at Webb Consolidated Independent School District in Bruni, TX - Final Performance Evaluation Report

    EPA Science Inventory

    This report documents the activities performed and the results obtained from the arsenic removal treatment technology demonstration project at the Webb Consolidated Independent School District (Webb CISD) in Bruni, TX. The main objective of the project was to evaluate the effect...

  10. The effect of water on the microstructure of 1-butyl-3-methylimidazolium tetrafluoroborate/TX-100/benzene ionic liquid microemulsions.

    PubMed

    Gao, Yan'an; Li, Na; Zheng, Liqiang; Zhao, Xueyan; Zhang, Jin; Cao, Quan; Zhao, Mingwei; Li, Zhen; Zhang, Gaoyong

    2007-01-01

    The ionic liquid (IL) 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][BF4]) forms nonaqueous microemulsions with benzene with the aid of nonionic surfactant TX-100. The phase diagram of the ternary system was prepared, and the microstructures of the microemulsion were recognized. On the basis of the phase diagram, a series of ionic liquid-in-oil (IL/O) microemulsions were chosen and characterized by dynamic light scattering (DLS), which shows a similar swelling behavior to typical water-in-oil (W/O) microemulsions. The existence of IL pools in the IL/O microemulsion was confirmed by UV/Vis spectroscopic analysis with CoCl2 and methylene blue (MB) as the absorption probes. A constant polarity of the IL pool is observed, even if small amounts of water are added to the microemulsion, thus suggesting that the water molecules are solubilized in the polar outer shell of the microemulsion, as confirmed by FTIR spectra. 1H NMR spectroscopic analysis shows that these water molecules interact with the electronegative oxygen atoms of the oxyethylene (OE) units of TX-100 through hydrogen-bonding interactions, and the electronegative oxygen atoms of the water molecules attract the electropositive imidazolium rings of [bmim][BF4]. Hence, the water molecules are like a glue that stick the IL and OE units more tightly together and thus make the microemulsion system more stable. Considering the unique solubilization behavior of added water molecules, the IL/O microemulsion system may be used as a medium to prepare porous or hollow nanomaterials by hydrolysis reactions.

  11. Role of solubilized water in the reverse ionic liquid microemulsion of 1-butyl-3-methylimidazolium tetrafluoroborate/TX-100/benzene.

    PubMed

    Gao, Yan'an; Li, Na; Zheng, Liqiang; Bai, Xiangtao; Yu, Li; Zhao, Xueyan; Zhang, Jin; Zhao, Mingwei; Li, Zhen

    2007-03-15

    The ionic liquid (IL) 1-butyl-3-methylimidazolium tetrafluoroborate (bmimBF4) can form nonaqueous microemulsions with benzene by the aid of nonionic surfactant TX-100. The effect of water on ionic liquid-in-oil (IL/O) microemulsions was studied, and it was shown that the addition of small amount of water to the IL microemulsion contributed to the stability of microemulsion and thus increased the amount of solubilized bmimBF4 in the microemulsion. The conductivity measurements also showed that the attractive interactions between IL microdroplets were weakened, that is, the IL/O microemulsion becomes more stable in the present of some water. Fourier transform IR was carried out to analyze the states of the added water, and the result showed that these water molecules mainly behaved as bound water and trapped water, indicating that the water molecules are located in the palisade layers of the IL/O microemulsion. Furthermore, 1H NMR and 19F NMR spectra suggested that the added water molecules built the hydrogen binding network of imidazolium cations and H2O, BF4- anion and H2O, and at the same time the electronegative oxygen atoms of the oxyethylene units of TX-100 and water in the palisade layers, which made the palisade layers more firm and thus increased the stability of the microemulsion. The study can help in further understanding the formation mechanism of microemulsions. In addition, the characteristic solubilization behavior of the added water can provide an aqueous interface film for hydrolysis reactions and therefore may be used as an ideal medium to prepare porous or hollow nanomaterials.

  12. The neuromuscular activity of Bothriopsis bilineata smaragdina (forest viper) venom and its toxin Bbil-TX (Asp49 phospholipase A2) on isolated mouse nerve-muscle preparations.

    PubMed

    Floriano, Rafael Stuani; Rocha, Thalita; Carregari, Victor Corasolla; Marangoni, Sergio; da Cruz-Höfling, Maria Alice; Hyslop, Stephen; Rodrigues-Simioni, Léa; Rowan, Edward G

    2015-03-01

    The presynaptic action of Bothriopsis bilineata smaragdina (forest viper) venom and Bbil-TX, an Asp49 PLA2 from this venom, was examined in detail in mouse phrenic nerve-muscle (PND) preparations in vitro and in a neuroblastoma cell line (SK-N-SH) in order to gain a better insight into the mechanism of action of the venom and associated Asp49 PLA2. In low Ca(2+) solution, venom (3μg/ml) caused a quadriphasic response in PND twitch height whilst at 10μg/ml the venom additionally induced an abrupt and marked initial contracture followed by neuromuscular facilitation, rhythmic oscillations of nerve-evoked twitches, alterations in baseline and progressive blockade. The venom slowed the relaxation phase of muscle twitches. In low Ca(2+), Bbil-TX [210nM (3μg/ml)] caused a progressive increase in PND twitch amplitude but no change in the decay time constant. Venom (10μg/ml) and Bbil-TX (210nM) caused minor changes in the compound action potential (CAP) amplitude recorded from sciatic nerve preparations, with no significant effect on rise time and latency; tetrodotoxin (3.1nM) blocked the CAP at the end of the experiments. In mouse triangularis sterni nerve-muscle (TSn-m) preparations, venom (10μg/ml) and Bbil-TX (210nM) significantly reduced the perineural waveform associated with the outward K(+) current while the amplitude of the inward Na(+) current was not significantly affected. Bbil-TX (210nM) caused a progressive increase in the quantal content of TSn-m preparations maintained in low Ca(2+) solution. Venom (3μg/ml) and toxin (210nM) increased the calcium fluorescence in SK-N-SH neuroblastoma cells loaded with Fluo3 AM and maintained in low or normal Ca(2+) solution. In normal Ca(2+), the increase in fluorescence amplitude was accompanied by irregular and frequent calcium transients. In TSn-m preparations loaded with Fluo4 AM, venom (10μg/ml) caused an immediate increase in intracellular Ca(2+) followed by oscillations in fluorescence and muscle contracture

  13. BmTx3, a scorpion toxin with two putative functional faces separately active on A-type K+ and HERG currents.

    PubMed Central

    Huys, Isabelle; Xu, Chen-Qi; Wang, Cheng-Zhong; Vacher, Hélène; Martin-Eauclaire, Marie-France; Chi, Cheng-Wu; Tytgat, Jan

    2004-01-01

    A novel HERG channel blocker was isolated from the venom of the scorpion Buthus martensi Karsch, sequenced and characterized at the pharmacological level after chemical synthesis. According to the determined amino acid sequence, the cDNA and genomic genes were then cloned. The genomic gene consists of two exons interrupted by an intron of 65 bp at position -6 upstream from the mature toxin. The protein sequence of this toxin was completely identical with that of a known A-type K+ current blocker BmTx3, belonging to scorpion alpha-KTx subfamily 15. Thus BmTx3 is the first reported alpha-KTx peptide also showing HERG-blocking activity, like gamma-KTx peptides. Moreover, different from classical alpha-KTx peptides, such as charybdotoxin, BmTx3 cannot block Shaker -type K+ channels. Phylogenetic tree analysis reveals that this toxin takes an intermediate position between classical alpha-KTx and gamma-KTx toxins. From a structural point of view, we propose that two separate functional faces might exist on the BmTx3 molecule, responsible for the two different K+-current-blocking functions. Face A, composed of Arg18 and Lys19 in the alpha-helix side, might correspond to HERG blocking activity, whereas Face B, containing a putative functional dyad (Lys27 and Tyr36) in the beta-sheet side, might correspond to A-type blocking activity. A specific deletion mutant with the disrupted Face B, BmTx3-Y36P37del, loses the A-type current-blocking activity, but keeps a similar HERG-blocking activity, as seen with the wild-type toxin. PMID:14599291

  14. Comparison of hypoxic cell radiosensitizers, KIN-804, KIN-844, KIN-806 and TX-1877, on brain and liver metabolizing capacities in mice bearing Ehrlich ascites carcinoma.

    PubMed

    Abou-Bedair, Farid Ahmed; Hori, Hitoshi; Nagasawa, Hideko; Uto, Yoshihiro; Abu-Zeid, Medhat; Inayama, Seiichi

    2002-05-01

    The biochemical effects of the 2-nitroimidazole hypoxic cell radiosensitizers KIN-804, KIN-806, and their analogues KIN-844 and TX-1877 on brain acetylcholinesterase (AChE) and hepatic free radical scavenging systems, such as reduced glutathione (GSH) and glucose-6-phosphate dehydrogenase (G-6-PDH) levels, and hepatic antioxidants, such as superoxide dismutase (SOD) and catalase, were evaluated in Ehrlich ascites carcinoma (EAC)-bearing Swiss albino mice. The assay of brain AChE revealed nonsignificant changes with all drugs examined. To evaluate the hepatic metabolic capacity, groups of mice were divided into control, EAC-inoculated, 10-Gy local gamma-irradiated, and KIN-804, KIN-844, KIN-806, or TX-1877 (50 mg/kg body weight, i.p.) groups, and gamma-irradiation was combined with each drug. EAC inoculation markedly suppressed GSH, G-6-PDH, SOD, and catalase levels. On the other hand, treatment with gamma-irradiation significantly enhanced them. The treatment of EAC-bearing mice with each drug alone in the absence of gamma-irradiation revealed that KIN-806 and its derivative TX-1877 showed antitumor activity through their significant recovery of GSH and SOD levels, respectively, in the EAC-bearing mice group. Similarly, the combined treatment of EAC-bearing mice with gamma-irradiation with each of the drugs tested showed that KIN-806 and TX-1877 significantly increased GSH and SOD, and to a lesser extent G-6-PDH and catalase levels. On the other hand, KIN-804 and KIN-844 had only a nonsignificant effect on all parameters examined. In conclusion, these data reveal that the administration of KIN-806 and TX-1877 with or without subsequent gamma-irradiation, resulted in significant recovery of GSH and SOD activities that were inhibited by EAC inoculation.

  15. Results of Phase I groundwater quality assessment for single-shell tank waste management areas T and TX-TY at the Hanford Site

    SciTech Connect

    Hodges, F.N.

    1998-01-01

    Pacific Northwest National Laboratory (PNNL) conducted a Phase I, Resource Conservation and Recovery Act of 1976 (RCRA) groundwater quality assessment for the Richland Field Office of the U.S. Department of Energy (DOE-RL) under the requirements of the Federal Facility Compliance Agreement. The purpose of the investigation was to determine if the Single-Shell Tank Waste Management Areas (WMAs) T and TX-TY have impacted groundwater quality. Waste Management Areas T and TX-TY, located in the northern part of the 200 West Area of the Hanford Site, contain the 241-T, 241-TX, and 241-TY tank farms and ancillary waste systems. These two units are regulated under RCRA interim-status regulations (under 40 CFR 265.93) and were placed in assessment groundwater monitoring because of elevated specific conductance in downgradient wells. Anomalous concentrations of technetium-99, chromium, nitrate, iodine-129, and cobalt-60 also were observed in some downgradient wells. Phase I assessment, allowed under 40 CFR 265, provides the owner-operator of a facility with the opportunity to show that the observed contamination has a source other than the regulated unit. For this Phase I assessment, PNNL evaluated available information on groundwater chemistry and past waste management practices in the vicinity of WMAs T and TX-TY. Background contaminant concentrations in the vicinity of WMAs T and TX-TY are the result of several overlapping contaminant plumes resulting from past-practice waste disposal operations. This background has been used as baseline for determining potential WMA impacts on groundwater.

  16. Vitamin D analogue TX 527 down-regulates the NF-κB pathway and controls the proliferation of endothelial cells transformed by Kaposi sarcoma herpesvirus

    PubMed Central

    González-Pardo, V; Verstuyf, A; Boland, R; Russo de Boland, A

    2013-01-01

    Background and Purpose The Kaposi sarcoma (KS)-associated herpesvirus GPCR (vGPCR) is a key molecule in the pathogenesis of KS, where it increases NF-κB gene expression and activates the NF-κB pathway. We investigated whether the less calcemic vitamin D analogue TX 527 inhibited the proliferation of endothelial cells transformed by vGPCR by modulation of the NF-κB pathway. Experimental Approach Endothelial cells transformed by vGPCR (SVEC-vGPCR) were treated with TX 527. Proliferation was measured by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) and cell cycle by flow cytometry. mRNA and protein levels were measured by real-time quantitative reverse transcriptase-PCR (qRT-PCR) and immunoblot analysis respectively. Key Results TX 527, similar to bortezomib (0.5 nM), a proteasome inhibitor that inhibits the activation of NF-κB, reduced proliferation and induced G0/G1 cell cycle arrest in SVEC-vGPCR. TX 527 like 1α,25(OH)2D3, biological active form of vitamin D, decreased the activity of NF-κB comparable with the effect of bortezomib. Time-response studies showed that TX 527 significantly decreased NF-κB and increased IκBα mRNA and protein levels. The increase of IκBα was accompanied by a reduction in p65/NF-κB translocation to the nucleus. These responses were abolished when vitamin D receptor (VDR) expression was suppressed by stable transfection of shRNA against VDR. In parallel with NF-κB inhibition, there was a down-regulation of inflammatory genes such as IL-6, CCL2/MCP and CCL20/MIP3α. Conclusions and Implications These results suggest that the anti-proliferative effects of the vitamin D analogue TX 527 in SVEC-vGPCR occur by modulation of the NF-κB pathway and are VDR dependent. PMID:23647513

  17. Differential effects of the recombinant toxin PnTx4(5-5) from the spider Phoneutria nigriventer on mammalian and insect sodium channels.

    PubMed

    Paiva, Ana L B; Matavel, Alessandra; Peigneur, Steve; Cordeiro, Marta N; Tytgat, Jan; Diniz, Marcelo R V; de Lima, Maria Elena

    2016-02-01

    The toxin PnTx4(5-5) from the spider Phoneutria nigriventer is extremely toxic/lethal to insects but has no macroscopic behavioral effects observed in mice after intracerebral injection. Nevertheless, it was demonstrated that it inhibits the N-methyl-d-aspartate (NMDA) - subtype of glutamate receptors of cultured rat hippocampal neurons. PnTx4(5-5) has 63% identity to PnTx4(6-1), another insecticidal toxin from P. nigriventer, which can slow down the sodium current inactivation in insect central nervous system, but has no effect on Nav1.2 and Nav1.4 rat sodium channels. Here, we have cloned and heterologous expressed the toxin PnTx4(5-5) in Escherichia coli. The recombinant toxin rPnTx4(5-5) was tested on the sodium channel NavBg from the cockroach Blatella germanica and on mammalian sodium channels Nav1.2-1.6, all expressed in Xenopus leavis oocytes. We showed that the toxin has different affinity and mode of action on insect and mammalian sodium channels. The most remarkable effect was on NavBg, where rPnTx4(5-5) strongly slowed down channel inactivation (EC50 = 212.5 nM), and at 1 μM caused an increase on current peak amplitude of 105.2 ± 3.1%. Interestingly, the toxin also inhibited sodium current on all the mammalian channels tested, with the higher current inhibition on Nav1.3 (38.43 ± 8.04%, IC50 = 1.5 μM). Analysis of activation curves on Nav1.3 and Nav1.5 showed that the toxin shifts channel activation to more depolarized potentials, which can explain the sodium current inhibition. Furthermore, the toxin also slightly slowed down sodium inactivation on Nav1.3 and Nav1.6 channels. As far as we know, this is the first araneomorph toxin described which can shift the sodium channel activation to more depolarized potentials and also slows down channel inactivation.

  18. Headspace vapor characterization of Hanford Waste Tank 241-TX-111: Results from samples collected on October 12, 1995. Tank Vapor Characterization Project

    SciTech Connect

    Pool, K.H.; Clauss, T.W.; Evans, J.C.

    1996-06-01

    This report describes the results of vapor samples taken from the headspace of waste storage tank 241-TX-111 (Tank TX-111) at the Hanford Site in Washington State. Pacific Northwest National Laboratory (PNNL) contracted with Westinghouse Hanford Company (WHC) to provide sampling devices and analyze samples for inorganic and organic analytes collected from the tank headspace and ambient air near the tank. The analytical work was performed by the PNNL Vapor Analytical Laboratory (VAL) by the Tank Vapor Characterization Project. Work performed was based on a sample and analysis plan (SAP) prepared by WHC. The SAP provided job-specific instructions for samples, analyses, and reporting. The SAP for this sample job was {open_quotes}Vapor Sampling and Analysis Plan{close_quotes}, and the sample job was designated S5069. Samples were collected by WHC on October 12, 1995, using the Vapor Sampling System (VSS), a truck-based sampling method using a heated probe inserted into the tank headspace.

  19. Comparative palynology of clastics and lignites from the Manning Formation, Jackson Group, Upper Eocene, Grimes County, TX

    SciTech Connect

    Gennett, J.A.

    1996-09-01

    The 3500 lignite seam at the Gibbons Creek Mine in Grimes County, TX was sampled for pollen and spores at 10 cm intervals. The majority of samples are dominated (to 60%) by Momipites from the Juglandaceae (walnut family), as is typical of Jackson Group sediments. Other palynomorph taxa vary systematically, with a peak of pollen of the freshwater tree Nyssa (blackgum) and associated Rboipites angustus (to 17%) occurring at the base. Higher in the seam, increase (to 55%) of Cupuliferoipollenites (a chestnut-like grain) and Cyrillaceae-pollenites? ventosus (to 7%) percentages may indicate a higher salinity environment. A Chrysophyllum (satin leaf) peak (to 25%) near the top of the seam suggests relatively shallow fresh-water conditions. Core samples from an interval above the lignites represent a transgressive-regressive cycle in inner shelf clastics. These samples were taken at 40 cm or greater intervals and reveal the regional pollen flora. Although minor changes occur, palynomorph spectra are for the most part homogenous. The dominant grain is again Momipites coryloides, but in general percentages are lower (to 35%). Cupuliferoipollenites (to 17%), Chrysophyllum (to 5%), and Rhoipites angustus (to 3%) are not less important, but do not peak as they do in the lignite spectra. Palm leaf megafossils; in one sample suggest a clastic wetland; in this sample palm pollen (mostly Arecipites, representing the modern saw palmetto) reaches 73%. Another sample contains high (26%) percentages of the fern spore Lygodiumsporites adriennis. High percentages of these two taxa do not occur in the lignite samples.

  20. Impacts of Near-term Climate Change on Surface Water - Groundwater Availability in the Nueces River basin, TX

    NASA Astrophysics Data System (ADS)

    Sinha, T.; Kumar, M.

    2014-12-01

    In arid and semi-arid regions, sustainability of surface water and groundwater resources is highly uncertain in the face of climate change as well as under competing demands due to urbanization, population growth and water needs to support ecosystem services. Most studies on climate change impact assessment focus on either surface water or groundwater resources alone. In this study, we utilize a fully coupled surface water and groundwater model, Penn-State Integrated Hydrologic Model (PIHM), and recent climate change projections from Climate Models Inter-comparison Project-5 (CMIP5) to evaluate impacts of near-term climate change on water availability in the Nueces River basin, TX. After performing calibration and validation of PIHM over multiple sites, hindcast simulations will be performed over the 1981-2010 period using data from multiple General Circulation Models (GCMs) obtained from the CMIP5 Project. The results will be compared to the observed data to understand added utility of hindcasts in improving the estimation of surface water and groundwater resources. Finally, we will assess the impacts of climate change on both surface water and groundwater resources over the next 20-30 years, which is a relevant time period for water management decisions.

  1. Spread of vaccinia virus through shaving during military training, Joint Base San Antonio-Lackland, TX, June 2014.

    PubMed

    Webber, Bryant J; Montgomery, Jay R; Markelz, Ana E; Allen, Kahtonna C; Hunninghake, John C; Ritchie, Simon A; Pawlak, Mary T; Johnston, Lindsay A; Oliver, Tiffany A; Winterton, Brad S

    2014-08-01

    Although naturally occurring smallpox virus was officially declared eradicated in 1980, concern for biological warfare prompted the U.S. Government in 2002 to recommend smallpox vaccination for select individuals. Vaccinia, the smallpox vaccine virus, is administered into the skin, typically on the upper arm, where the virus remains viable and infectious until the scab falls off and the epidermis is fully intact - typically 2-4 weeks. Adverse events following smallpox vaccination may occur in the vaccinee, in individuals who have contact with the vaccinee (i.e., secondary transmission), or in individuals who have contact with the vaccinee's contact (i.e., tertiary transmission). In June 2014 at Joint Base San Antonio-Lackland, TX, two cases of inadvertent inoculation of vaccinia and one case of a non-viral reaction following vaccination occurred in the security forces training squadron. This includes the first reported case of shaving as the likely source of autoinoculation after contact transmission. This paper describes the diagnosis and treatment of these cases, the outbreak investigation, and steps taken to prevent future transmission.

  2. Structural and Phylogenetic Studies with MjTX-I Reveal a Multi-Oligomeric Toxin – a Novel Feature in Lys49-PLA2s Protein Class

    PubMed Central

    Cavalcante, Walter L. G.; Fernandez, Roberto M.; Gallacci, Márcia; Soares, Andreimar M.; Oliveira, Cristiano L. P.; Fontes, Marcos R. M.

    2013-01-01

    The mortality caused by snakebites is more damaging than many tropical diseases, such as dengue haemorrhagic fever, cholera, leishmaniasis, schistosomiasis and Chagas disease. For this reason, snakebite envenoming adversely affects health services of tropical and subtropical countries and is recognized as a neglected disease by the World Health Organization. One of the main components of snake venoms is the Lys49-phospholipases A2, which is catalytically inactive but possesses other toxic and pharmacological activities. Preliminary studies with MjTX-I from Bothrops moojeni snake venom revealed intriguing new structural and functional characteristics compared to other bothropic Lys49-PLA2s. We present in this article a comprehensive study with MjTX-I using several techniques, including crystallography, small angle X-ray scattering, analytical size-exclusion chromatography, dynamic light scattering, myographic studies, bioinformatics and molecular phylogenetic analyses.Based in all these experiments we demonstrated that MjTX-I is probably a unique Lys49-PLA2, which may adopt different oligomeric forms depending on the physical-chemical environment. Furthermore, we showed that its myotoxic activity is dramatically low compared to other Lys49-PLA2s, probably due to the novel oligomeric conformations and important mutations in the C-terminal region of the protein. The phylogenetic analysis also showed that this toxin is clearly distinct from other bothropic Lys49-PLA2s, in conformity with the peculiar oligomeric characteristics of MjTX-I and possible emergence of new functionalities inresponse to environmental changes and adaptation to new preys. PMID:23573271

  3. Microemulsions with surfactant TX100, cyclohexane, and an ionic liquid investigated by conductance, DLS, FTIR measurements, and study of solvent and rotational relaxation within this microemulsion.

    PubMed

    Pramanik, Rajib; Sarkar, Souravi; Ghatak, Chiranjib; Rao, Vishal Govind; Setua, Palash; Sarkar, Nilmoni

    2010-06-10

    Room-temperature ionic liquids (RTILs), N,N,N-trimethyl-N-propyl ammonium bis(trifluoromethanesulfonyl) imide ([N(3111)][Tf(2)N]), were substituted for polar water and formed nonaqueous microemulsions with cyclohexane by the aid of nonionic surfactant TX-100. The phase behavior of the ternary system was investigated, and microregions of [N(3111)][Tf(2)N]-in-cyclohexane (IL/O), bicontinuous, and cyclohexane-in-[N(3111)][Tf(2)N] (O/IL) were identified by traditional electrical conductivity measurements. Dynamic light scattering (DLS) revealed the formation of the IL microemulsions. The FTIR study of O-H stretching band of TX100 also supports this finding. The dynamics of solvent and rotational relaxation have been investigated in [N(3111)][Tf(2)N]/TX100/cyclohexane microemulsions using steady-state and time-resolved fluorescence spectroscopy as a tool and coumarin 480 (C-480) as a fluorescence probe. The size of the microemulsions increases with gradual addition of [N(3111)][Tf(2)N], which revealed from DLS measurement. This leads to the faster collective motions of cation and anions of [N(3111)][Tf(2)N], which contributes to faster solvent relaxation in microemulsions.

  4. Intermediate valence to heavy fermion through a quantum phase transition in Yb3(Rh1-xTx)4Ge13 (T = Co, Ir) single crystals

    NASA Astrophysics Data System (ADS)

    Rai, Binod; Morosan, Emilia

    Single crystals of Yb3(Rh1-x Tx)4Ge13 (T = Co, Ir) have been grown using the self-flux method. Powder X-ray diffraction data on these compounds are consistent with the cubic structure with space group Pm 3 n . Intermediate valence behavior is observed in Yb3(Rh1-x Tx)4Ge13 upon T = Co doping, while T = Ir doping drives the system into a heavy fermion state. Antiferromagnetic order is observed in the Ir-doped samples Yb3(Rh1-x Tx)4Ge13 for 0.5 < x <= 1 with TN = 0.96 K for Yb3Ir4Ge13. With decreasing x, the magnetic order is suppressed towards a quantum critical point around xc = 0.5, accompanied by non-Fermi liquid behavior evidenced by logarithmic divergence of the specific heat and linear temperature dependence of the resistivity. The Fermi liquid behavior is recovered with the application of large magnetic fields. Gordon and Betty Moore Foundation EPiQS initiative through Grant GBMF4417 and Welch Foundation.

  5. Quantification of 1-(propan-2-ylamino)-4-propoxy-9H-thioxanthen-9-one (TX5), a newly synthetized P-glycoprotein inducer/activator, in biological samples: method development and validation.

    PubMed

    Ferreira, Ana Filipa; Ponte, Filipa; Silva, Renata; Rocha-Pereira, Carolina; Sousa, Emília; Pinto, Madalena; Bastos, Maria de Lourdes; Remião, Fernando

    2017-02-01

    A simple, rapid and economical method was developed and validated for the analysis and quantification of 1-(propan-2-ylamino)-4-propoxy-9H-thioxanthen-9-one (TX5), a P-glycoprotein inducer/activator, in biological samples, using reverse-phase high-performance liquid chromatography (HPLC). A C18 column and a mobile phase composed of methanol-water (90/10, v/v) with 1% (v/v) triethylamine, at a flow rate of 1 mL/min, were used for chromatographic separation. TX5 standards (0.5-150 μm) were prepared in human serum. Methanol was used for TX5 extraction and serum protein precipitation. After filtration, samples were injected into the HPLC apparatus and TX5 was quantified by a conventional UV detector at 255 nm. The TX5 retention time was 13 min in this isocratic system. The method was validated according to ICH guidelines for specificity/selectivity, linearity, accuracy, precision, limits of detection and quantification (LOD and LOQ) and recovery. The method was proved to be selective, as there were no interferences of endogenous compounds with the same retention time of TX5. Also, the developed method was linear (r(2)  ≥ 0.99) for TX5 concentrations between 0.5 and 150 μm and the LOD and LOQ were 0.08 and 0.23 μm, respectively. The results indicated that the reported method could meet the requirements for TX5 analysis in the trace amounts expected to be present in biological samples.

  6. Misexpression of AtTX12 encoding a Toll/interleukin-1 receptor domain induces growth defects and expression of defense-related genes partially independently of EDS1 in Arabidopsis

    PubMed Central

    Song, Sang-Kee

    2016-01-01

    In this study, a tissue-specific GAL4/UAS activation tagging system was used for the characterization of genes which could induce lethality when ubiquitously expressed. A dominant mutant exhibiting stunted growth was isolated and named defective root development 1-D (drd1-D). The T-DNA tag was located within the promoter region of AtTX12, which is predicted to encode a truncated nucleotide-binding leucine-rich repeat (NLR) protein, containing a Toll/interleukin-1 receptor (TIR) domain. The transcript levels of AtTX12 and defense-related genes were elevated in drd1-D, and the misexpression of AtTX12 recapitulated the drd1-D phenotypes. In the presence of ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1), a key transducer of signals triggered by TIR-type NLRs, a low-level of AtTX12 misexpression induced strong defective phenotypes including seedling lethality whereas, in the absence of EDS1, a high-level of AtTX12 misexpression induced weak growth defects like dwarfism, suggesting that AtTX12 might function mainly in an EDS1-dependent and partially in an EDS1-independent manner. [BMB Reports 2016; 49(12): 693–698] PMID:27802841

  7. Cell cycle arrest and apoptosis induced by 1α,25(OH)2D3 and TX 527 in Kaposi sarcoma is VDR dependent.

    PubMed

    González-Pardo, Verónica; Suares, Alejandra; Verstuyf, Annemieke; De Clercq, Pierre; Boland, Ricardo; de Boland, Ana Russo

    2014-10-01

    We have previously shown that 1α,25(OH)2-Vitamin D3 [1α,25(OH)2D3] and its less calcemic analog TX 527 inhibit the proliferation of endothelial cells transformed by the viral G protein-coupled receptor associated to Kaposi sarcoma (vGPCR) and this could be partially explained by the inhibition of the NF-κB pathway. In this work, we further explored the mechanism of action of both vitamin D compounds in Kaposi sarcoma. We investigated whether the cell cycle arrest and subsequent apoptosis of endothelial cells (SVEC) and SVEC transformed by vGPCR (SVEC-vGPCR) elicited by 1α,25(OH)2D3 and TX 527 were mediated by the vitamin D receptor (VDR). Cell cycle analysis of SVEC and SVEC-vGPCR treated with 1α,25(OH)2D3 (10nM, 48h) revealed that 1α,25(OH)2D3 increased the percentage of cells in the G0/G1 phase and diminished the percentage of cells in the S phase of the cell cycle. Moreover, the number of cells in the S phase was higher in SVEC-vGPCR than in SVEC due to vGPCR expression. TX 527 exerted similar effects on growth arrest in SVEC-vGPCR cells. The cell cycle changes were suppressed when the expression of the VDR was blocked by a stable transfection of shRNA against VDR. Annexin V-PI staining demonstrated apoptosis in both SVEC and SVEC-vGPCR after 1α,25(OH)2D3 and TX 527 treatment (10nM, 24h). Cleavage of caspase-3 detected by Western blot analysis was increased to a greater extent in SVEC than in SVEC-vGPCR cells, and this effect was also blocked in VDR knockdown cells. Altogether, these results suggest that 1α,25(OH)2D3 and TX 527 inhibit the proliferation of SVEC and SVEC-vGPCR and induce apoptosis by a mechanism that involves the VDR.

  8. On the structure of hot gas in haloes: implications for the LX-TX relation and missing baryons

    NASA Astrophysics Data System (ADS)

    Sharma, Prateek; McCourt, Michael; Parrish, Ian J.; Quataert, Eliot

    2012-12-01

    We present one-dimensional models of the hot gas in dark matter haloes, which both predict the existence of cool cores and explain their structure. Our models are directly applicable to semi-analytic models of galaxy formation. We have previously argued that filaments of cold (˜104 K) gas condense out of the intracluster medium (ICM) in hydrostatic and thermal equilibrium when the ratio of the thermal instability time-scale to the free-fall time tTI/tff falls below 5-10. This criterion corresponds to an upper limit on the density of the ICM and motivates a model in which a density core forms wherever tTI/tff ≲ 10. Consistent with observations and numerical simulations, this model predicts larger and more tenuous cores for lower mass haloes - while the core density in a cluster may be as large as ˜0.1 cm-3, the core density in the Galactic halo should not exceed ˜10-4 cm-3. We can also explain the large densities in smaller mass haloes (galactic 'coronae') if we include the contribution of the central galaxy to the gravitational potential. Our models produce a favourable match to the observational X-ray luminosity-temperature (LX-TX) relation. For halo masses ≲1013 M⊙ the core size approaches the virial radius. Thus, most of the baryons in such haloes cannot be in the hot ICM, but either in the form of stars or in the form of hot gas beyond the virial radius. Because of the smaller mass in the ICM and much larger mass available for star formation, the majority of the baryons in low-mass haloes (≲1013 M⊙) can be expelled beyond the virial radius due to supernova feedback. This can account for the baryons 'missing' from low-mass haloes, such as the Galactic halo.

  9. Integrating Infrastructure-Relevant Climate Projections into City Planning: Learning from Boulder CO, Austin TX and Washington DC

    NASA Astrophysics Data System (ADS)

    Stoner, A. M. K.; Hayhoe, K.

    2015-12-01

    Over the coming century, climate change has the potential to impact infrastructure in many different ways, particularly in population-dense areas that depend on transportation and built environments. Many of these impacts may occur via changes in the frequency and magnitude of extremes: high and low temperature, heat waves, precipitation, coastal flooding, and storm events. Having a better idea of how the climate might change locally, both within the near future as well as toward the end of the century, can give city planners and engineers guidance when designing new structures and when repairing and fortifying existing components of buildings, bridges, highways, sewers, ports, etc. However, the type of event and the amount of damages that may be incurred are often highly specific to a given location. Over the last 10 years, we have worked with a broad range of cities, states, non-profit organizations, and federal agencies to integrate climate projections into ongoing resiliency, sustainability, and management processes. Drawing on that experience, we describe the broad steps in assimilating climate information into existing decision-making frameworks relevant to most applications, as well as highlighting many of the unique aspects of these analyses using examples from our most recent work with three very different cities - Austin TX, Boulder CO and Washington DC. From initial conversations with local experts to identify relevant thresholds to final integration of projected changes into the planning processes of these cities, these case studies highlight the utility of including future climate projections into infrastructure planning, the challenges to doing so, and the over-arching importance of communication and interaction between infrastructure experts, engineers, and scientists.

  10. The Impacts of Exposure to Environmental Risk on Physical and Mental Health in a Small Geographic Community in Houston, TX.

    PubMed

    Sansom, Garett; Parras, Juan; Parras, Ana; Nieto, Yudith; Arellano, Yvette; Berke, Philip; McDonald, Thomas; Shipp, Eva; Horney, Jennifer A

    2017-03-13

    Previous research has shown that communities with low average socioeconomic status (SES) and majority minority populations are more likely to be exposed to industrial buildings, waste facilities, and poor infrastructure compared to white communities with higher average SES. While some studies have demonstrated linkages between exposures to specific environmental contaminates within these communities and negative health outcomes, little research has analyzed the effects of environmental contaminants on the mental and physical health of these populations. A cross-sectional survey collected data from residents of Manchester, a small neighborhood in Houston, TX, that is characterized by industrial sites, unimproved infrastructure, nuisance flooding, and poor air quality. Our study (N = 109) utilized the 12 item Short Form Health Survey version 2 (SF12v2) to assess the general mental and physical health of the community. The community as a whole had reduced physical health scores compared to U.S. national averages. The time residents had lived in the neighborhood was also correlated with a reported reduction in physical health scores (r2 = 0.136; p-value <0.001). The association between time lived in the neighborhood and poorer health scores remained after adjusting for age, race, and gender (coef = -0.27, p-value <0.001). Mental health scores were within national averages and time spent living in the neighborhood did not appear to negatively impact respondent's mental health scores. These findings point to the need for more research to determine the potential for additive physical and mental health impacts in long-term residents in neighborhoods characterized by environmental justice issues.

  11. Focalyx Dx, Bx, Tx et Apps: A novel contemporary fusion paradigm for the management of prostate cancer.

    PubMed

    Bianco, F J; Martínez-Salamanca, J I

    2016-07-01

    FocalyxTM conceived as a response to emerging evidence data across numerous cancer lesions that questions current standard treatment approaches that too often lead to detrimental quality of life yet delivering limited survival benefit, especially in-lieu of advances in imaging technology applicable to cancer patients. The Focalyx paradigm aims to control cancer with improvement in quality of life. We initially devised 5 milestones: 1- Consistently optimize Prostate MRI imaging using the novel published protocols adopted as guidelines by societies such as the European Society of Urology and Radiology; 2- Evaluate fusion platform software solutions that existed; 3- Determine best fusión platform for Focalyx on practicality, precision, and workflow premises; 4- Evaluate commercially available FDA approved ablative technologies to implement our treatment vision; 5- Design a treatment option that can be performed in the office setting under local anesthesia, which would not impact negatively QOL outcomes of Prostate Cancer patients and seamless constant nonintrusive practical patient-physician interaction by the Focalyx app that facilitates follow up and provides early warning signals shall any change in the disease dynamics emerge. Prostate cancer was identified as the pilot disease for Focalyx to deliver a "GPS" like solution for the prostate gland that destroys identifiable disease without adverse effects such as: cancer anxiety, urinary incontinence, loss of erections and ejaculation. Since September of 2013, over 300 men have been accrued in NCT02381990- clintrials.gov evaluating the feasibility of our solutions for imaging (FocalyxDx), Biopsy (FocalyxBx) and Treatment (FocalyxTx). In this review we detail the tools available to achieve the Focalyx paradigm for men with Prostate Cancer.

  12. Impacts of cool cities on air quality: A preliminary modeling assessment for Nashville TN, Dallas TX and Atlanta GA

    SciTech Connect

    Taha, Haider

    1998-06-15

    Previous atmospheric modeling efforts that concentrated on the Los Angeles Basin suggested beneficial and significant air quality impacts from cool cities strategies. This paper discusses an extension of similar modeling efforts to three regions, Atlanta GA, Dallas - Ft. Worth TX, and Nashville TN, that experience smog and air quality problems. According to the older ozone air quality standard (120 ppb), these regions were classified as serious, moderate, and marginal, respectively, but may be out of compliance with respect to the newer, 80-ppb/8-hours standard. Results from this exploratory modeling work suggest a range of possible impacts on meteorological and air quality conditions. For example, peak ozone concentrations during each region's respective episode could be decreased by 1-6 ppb (conservative and optimistic scenarios, respectively) in Nashville, 5-15 ppb in Dallas - Fort Worth, and 5-12 ppb in Atlanta following implementation of cool cities. The reductions are generally smaller than those obtained from simulating the Los Angeles Basin but are still significant. In all regions, the simulations suggest, the net, domain-wide effects of cool cities are reductions in ozone mass and improvements in air quality. In Atlanta, Nashville, and Dallas, urban areas benefiting from reduced smog reach up to 8460, 7350, and 12870 km{sup 2} in area, respectively. Results presented in this paper should be taken as exploratory and preliminary. These will most likely change during a more comprehensive modeling study to be started soon with the support of the US Environmental Protection Agency. The main purpose of the present project was to obtain the initial data (emission inventories) for these regions, simulate meteorological conditions, and perform preliminary sensitivity analysis. In the future, additional regions will be simulated to assess the potential of cool cities in improving urban air quality.

  13. Resolving the structure of Ti3C2Tx MXenes through multilevel structural modeling of the atomic pair distribution function

    DOE PAGES

    Wesolowski, David J.; Wang, Hsiu -Wen; Page, Katharine L.; ...

    2015-12-08

    MXenes are a recently discovered family of two-dimensional (2D) early transition metal carbides and carbonitrides, which have already shown many attractive properties and a great promise in energy storage and many other applications. However, a complex surface chemistry and small coherence length has been an obstacle in some applications of MXenes, also limiting accuracy of predictions of their properties. In this study, we describe and benchmark a novel way of modeling layered materials with real interfaces (diverse surface functional groups and stacking order between the adjacent monolayers) against experimental data. The structures of three kinds of Ti3C2Tx MXenes (T standsmore » for surface terminating species, including O, OH, and F) produced under different synthesis conditions were resolved for the first time using atomic pair distribution function obtained by high-quality neutron total scattering. The true nature of the material can be easily captured with the sensitivity of neutron scattering to the surface species of interest and the detailed third-generation structure model we present. The modeling approach leads to new understanding of MXene structural properties and can replace the currently used idealized models in predictions of a variety of physical, chemical and functional properties of Ti3C2-based MXenes. Furthermore, the developed models can be employed to guide the design of new MXene materials with selected surface termination and controlled contact angle, catalytic, optical, electrochemical and other properties. We suggest that the multi-level structural modeling should form the basis for a generalized methodology on modeling diffraction and pair distribution function data for 2D and layered materials.« less

  14. Mineralogical analysis of two different kind of icy surfaces in the trans-neptunian belt, TNOs (50000) Quaoar and 2002 TX300

    NASA Astrophysics Data System (ADS)

    Pinilla-Alonso, N.; Licandro, J.; Campins, H.

    2004-11-01

    TNOs (50000) Quaoar and 2002 TX300 were observed in the framework of a long-term program to study the surface properties of TNOs, Centaurs and comet nuclei using the Italian 3.58 m Telescopio Nazionale Galileo (TNG) at "El Roque de los Muchachos" Observatory (La Palma, Spain). Near-infrared spectra of both TNOs and visible spectrum of 2002 TX300 were obtained. Combining our spectra and the visible spectrum of (50000) Quaoar done by Marchi et al. (2004, A&A, 408, 17), and using the simple one-dimensional geometrical-optics formulation by Shkuratov et al. (1999, Icarus 137, 235), the visible and near-infrared spectra covering the 0.5-2.4 μ m range of both TNOs are analyzed in order to obtain mineralogical information of their surfaces. A large fraction of big water ice particles is needed to reproduce the large absorption bands observed in both objects, while other component, may be methanol, is needed to reproduce the absorption around 2.2 μ m observed in Quaoar (also reported by Brown & Trujillo 2004, AJ in press). Quaoar surface is very red, and a large fraction of complex organics is needed to reproduce the slope and general shape of the spectrum. On the other hand, the neutral color of the surface of TX300 and the huge water ice absorption bands suggest a very low abundance of complex organics. The very different fraction of tholins and water ice in the surface of both objects is indicative either of a very different original composition or a different resurfacing history. The results are discussed in the framework of the different resurfacing models already proposed. This work was supported by the grants from the National Science Foundation and the NASA Planetary Astronomy program.

  15. SU-E-J-48: Imaging Origin-Radiation Isocenter Coincidence for Linac-Based SRS with Novalis Tx

    SciTech Connect

    Geraghty, C; Workie, D; Hasson, B

    2015-06-15

    Purpose To implement and evaluate an image-based Winston-Lutz (WL) test to measure the displacement between ExacTrac imaging origin and radiation isocenter on a Novalis Tx system using RIT V6.2 software analysis tools. Displacement between imaging and radiation isocenters was tracked over time. The method was applied for cone-based and MLC-based WL tests. Methods The Brainlab Winston-Lutz phantom was aligned to room lasers. The ExacTrac imaging system was then used to detect the Winston- Lutz phantom and obtain the displacement between the center of the phantom and the imaging origin. EPID images of the phantom were obtained at various gantry and couch angles and analyzed with RIT calculating the phantom center to radiation isocenter displacement. The RIT and Exactrac displacements were combined to calculate the displacement between imaging origin and radiation isocenter. Results were tracked over time. Results Mean displacements between ExacTrac origin and radiation isocenter were: VRT: −0.1mm ± 0.3mm, LNG: 0.5mm ± 0.2mm, LAT: 0.2mm ± 0.2mm (vector magnitude of 0.7 ± 0.2mm). Radiation isocenter was characterized by the mean of the standard deviations of the WL phantom displacements: σVRT: 0.2mm, σLNG: 0.4mm, σLAT: 0.6mm. The linac couch base was serviced to reduce couch walkout. This reduced σLAT to 0.2mm. These measurements established a new baseline of radiation isocenter-imaging origin coincidence. Conclusion The image-based WL test has ensured submillimeter localization accuracy using the ExacTrac imaging system. Standard deviations of ExacTrac-radiation isocenter displacements indicate that average agreement within 0.3mm is possible in each axis. This WL test is a departure from the tradiational WL in that imaging origin/radiation isocenter agreement is the end goal not lasers/radiation isocenter.

  16. Exploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake venom.

    PubMed

    Resende, L M; Almeida, J R; Schezaro-Ramos, R; Collaço, R C O; Simioni, L R; Ramírez, D; González, W; Soares, A M; Calderon, L A; Marangoni, S; da Silva, S L

    2017-03-01

    Phospholipases A2 (PLA2s) constitute a class of extensively studied toxins, isolated from snake venoms. Basic PLA2 isoforms mediate various toxicological effects, while the acidic isoforms generally have higher enzymatic activities, but do not promote evident toxic effects. The functions of these acidic isoforms in snake venoms are still not completely understood and more studies are needed to characterize the biological functions and diversification of acidic toxins in order to justify their abundant presence in these secretions. Recently, Lomonte and collaborators demonstrated, in a proteomic and toxicological study, high concentrations of PLA2s in the venom of Agkistrodon piscivorus leucostoma. We have, herein, purified and characterized an acidic PLA2 from this snake venom, denominated AplTx-I, in order to better understand its biochemical and structural characteristics, as well as its biological effects. AplTx-I was purified using two chromatographic steps, in association with enzymatic and biological assays. The acidic toxin was found to be one of the most abundant proteins in the venom of A. p. leucostoma; the protein was monomeric with a molecular mass of 13,885.8 Da, as identified by mass spectrometry ESI-TOF and electrophoresis. The toxin has similar primary and tridimensional structures to those of other acidic PLA2s, a theoretical and experimental isoelectric point of ≈5.12, and a calcium-dependent enzyme activity of 25.8985 nM/min/mg, with maximum values at 37 °C and pH 8.0. Despite its high enzymatic activity on synthetic substrate, AplTx-I did not induce high or significant myotoxic, coagulant, anticoagulant, edema, neuromuscular toxicity in mouse phrenic nerve-diaphragm preparations or antibacterial activities. Interestingly, AplTx-I triggered a high and selective neuromuscular toxicity in chick biventer cervicis preparations. These findings are relevant to provide a deeper understanding of the pharmacology, role and diversification of acidic

  17. Feasibility of using Geant4 Monte Carlo simulation for IMRT dose calculations for the Novalis Tx with a HD-120 multi-leaf collimator

    NASA Astrophysics Data System (ADS)

    Jung, Hyunuk; Shin, Jungsuk; Chung, Kwangzoo; Han, Youngyih; Kim, Jinsung; Choi, Doo Ho

    2015-05-01

    The aim of this study was to develop an independent dose verification system by using a Monte Carlo (MC) calculation method for intensity modulated radiation therapy (IMRT) conducted by using a Varian Novalis Tx (Varian Medical Systems, Palo Alto, CA, USA) equipped with a highdefinition multi-leaf collimator (HD-120 MLC). The Geant4 framework was used to implement a dose calculation system that accurately predicted the delivered dose. For this purpose, the Novalis Tx Linac head was modeled according to the specifications acquired from the manufacturer. Subsequently, MC simulations were performed by varying the mean energy, energy spread, and electron spot radius to determine optimum values of irradiation with 6-MV X-ray beams by using the Novalis Tx system. Computed percentage depth dose curves (PDDs) and lateral profiles were compared to the measurements obtained by using an ionization chamber (CC13). To validate the IMRT simulation by using the MC model we developed, we calculated a simple IMRT field and compared the result with the EBT3 film measurements in a water-equivalent solid phantom. Clinical cases, such as prostate cancer treatment plans, were then selected, and MC simulations were performed. The accuracy of the simulation was assessed against the EBT3 film measurements by using a gamma-index criterion. The optimal MC model parameters to specify the beam characteristics were a 6.8-MeV mean energy, a 0.5-MeV energy spread, and a 3-mm electron radius. The accuracy of these parameters was determined by comparison of MC simulations with measurements. The PDDs and the lateral profiles of the MC simulation deviated from the measurements by 1% and 2%, respectively, on average. The computed simple MLC fields agreed with the EBT3 measurements with a 95% passing rate with 3%/3-mm gamma-index criterion. Additionally, in applying our model to clinical IMRT plans, we found that the MC calculations and the EBT3 measurements agreed well with a passing rate of greater

  18. Characterization of Vadose Zone Sediments Below the TX Tank Farm: Boreholes C3830, C3831, C3832 and RCRA Borehole 299-W10-27

    SciTech Connect

    Serne, R. Jeffrey; Bjornstad, Bruce N.; Horton, Duane G.; Lanigan, David C.; Lindenmeier, Clark W.; Lindberg, Michael J.; Clayton, Ray E.; Legore, Virginia L.; Orr, Robert D.; Kutnyakov, Igor V.; Baum, Steven R.; Geiszler, Keith N.; Valenta, Michelle M.; Vickerman, Tanya S.

    2008-09-11

    This report was revised in September 2008 to remove acid-extractable sodium data from Tables 4.8, 4.28,4.43, and 4.59. The sodium data was removed due to potential contamination introduced during the acid extraction process. The rest of the text remains unchanged from the original report issued in April 2004. The overall goal of the Tank Farm Vadose Zone Project, led by CH2M HILL Hanford Group, Inc., is to define risks from past and future single-shell tank farm activities at Hanford. To meet this goal, CH2M HILL Hanford Group, Inc. tasked scientists from Pacific Northwest National Laboratory to perform detailed analyses on vadose zone sediments from within Waste Management Area (WMA) T-TX-TY. This report is the first of two reports written to present the results of these analyses. Specifically, this report contains all the geologic, geochemical, and selected physical characterization data collected on vadose zone sediment recovered from boreholes C3830, C3831, and C3832 in the TX Tank Farm, and from borehole 299-W-10-27 installed northeast of the TY Tank Farm.

  19. Single Residue Substitutions That Confer Voltage-Gated Sodium Ion Channel Subtype Selectivity in the NaV1.7 Inhibitory Peptide GpTx-1.

    PubMed

    Murray, Justin K; Long, Jason; Zou, Anruo; Ligutti, Joseph; Andrews, Kristin L; Poppe, Leszek; Biswas, Kaustav; Moyer, Bryan D; McDonough, Stefan I; Miranda, Les P

    2016-03-24

    There is interest in the identification and optimization of new molecular entities selectively targeting ion channels of therapeutic relevance. Peptide toxins represent a rich source of pharmacology for ion channels, and we recently reported GpTx-1 analogs that inhibit NaV1.7, a voltage-gated sodium ion channel that is a compelling target for improved treatment of pain. Here we utilize multi-attribute positional scan (MAPS) analoging, combining high-throughput synthesis and electrophysiology, to interrogate the interaction of GpTx-1 with NaV1.7 and related NaV subtypes. After one round of MAPS analoging, we found novel substitutions at multiple residue positions not previously identified, specifically glutamic acid at positions 10 or 11 or lysine at position 18, that produce peptides with single digit nanomolar potency on NaV1.7 and 500-fold selectivity against off-target sodium channels. Docking studies with a NaV1.7 homology model and peptide NMR structure generated a model consistent with the key potency and selectivity modifications mapped in this work.

  20. Functional and pharmacological characterization of two different ASIC1a/2a heteromers reveals their sensitivity to the spider toxin PcTx1

    PubMed Central

    Joeres, Niko; Augustinowski, Katrin; Neuhof, Andreas; Assmann, Marc; Gründer, Stefan

    2016-01-01

    Acid Sensing Ion Channels (ASICs) detect extracellular proton signals and are involved in synaptic transmission and pain sensation. ASIC subunits assemble into homo- and heteromeric channels composed of three subunits. Single molecule imaging revealed that heteromers composed of ASIC1a and ASIC2a, which are widely expressed in the central nervous system, have a flexible 2:1/1:2 stoichiometry. It was hitherto not possible, however, to functionally differentiate these two heteromers. To have a homogenous population of ASIC1a/2a heteromers with either 2:1 or 1:2 stoichiometry, we covalently linked subunits in the desired configuration and characterized their functional properties in Xenopus oocytes. We show that the two heteromers have slightly different proton affinity, with an additional ASIC1a subunit increasing apparent affinity. Moreover, we found that zinc, which potentiates ASIC2a-containing ASICs but not homomeric ASIC1a, potentiates both heteromers. Finally, we show that PcTx1, which binds at subunit-subunit interfaces of homomeric ASIC1a, inhibits both heteromers suggesting that ASIC2a can also contribute to a PcTx1 binding site. Using this functional fingerprint, we show that rat cortical neurons predominantly express the ASIC1a/2a heteromer with a 2:1 stoichiometry. Collectively, our results reveal the contribution of individual subunits to the functional properties of ASIC1a/2a heteromers. PMID:27277303

  1. Molecular interactions of the gating modifier toxin ProTx-II with NaV 1.5: implied existence of a novel toxin binding site coupled to activation.

    PubMed

    Smith, Jaime J; Cummins, Theodore R; Alphy, Sujith; Blumenthal, Kenneth M

    2007-04-27

    Voltage-gated Na(+) channels are critical components in the generation of action potentials in excitable cells, but despite numerous structure-function studies on these proteins, their gating mechanism remains unclear. Peptide toxins often modify channel gating, thereby providing a great deal of information about these channels. ProTx-II is a 30-amino acid peptide toxin from the venom of the tarantula, Thrixopelma pruriens, that conforms to the inhibitory cystine knot motif and which modifies activation kinetics of Na(v) and Ca(v), but not K(v), channels. ProTx-II inhibits current by shifting the voltage dependence of activation to more depolarized potentials and, therefore, differs from the classic site 4 toxins that shift voltage dependence of activation in the opposite direction. Despite this difference in functional effects, ProTx-II has been proposed to bind to neurotoxin site 4 because it modifies activation. Here, we investigate the bioactive surface of ProTx-II by alanine-scanning the toxin and analyzing the interactions of each mutant with the cardiac isoform, Na(v)1.5. The active face of the toxin is largely composed of hydrophobic and cationic residues, joining a growing group of predominantly K(v) channel gating modifier toxins that are thought to interact with the lipid environment. In addition, we performed extensive mutagenesis of Na(v)1.5 to locate the receptor site with which ProTx-II interacts. Our data establish that, contrary to prior assumptions, ProTx-II does not bind to the previously characterized neurotoxin site 4, thus making it a novel probe of activation gating in Na(v) channels with potential to shed new light on this process.

  2. Effect of alkyl chain of room temperature ionic liquid (RTILs) on the phase behavior of [C2mim][C(n)SO4]/TX-100/cyclohexane microemulsions: solvent and rotational relaxation study.

    PubMed

    Ghosh, Surajit; Banerjee, Chiranjib; Mandal, Sarthak; Rao, Vishal Govind; Sarkar, Nilmoni

    2013-05-16

    In this investigation, we present microemulsions comprising a nonionic surfactant, Triton X-100 (TX-100), cyclohexane as nonpolar phase, and room temperature ionic liquids (RTILs) as a polar medium. To investigate the effect of alkyl chain length of ionic liquid on the physicochemical properties of microemulsions, we have used 1-ethyl-3-methylimidazolium n-butyl sulfate [C2mim][C4SO4], 1-ethyl-3-methylimidazolium n-hexyl sulfate [C2mim][C6SO4], and 1-ethyl-3-methylimidazolium n-octyl sulfate [C2mim][C8SO4] as polar media. The phase behavior of these ternary systems is investigated by direct observation of transition from clear transparent solution to turbid solution by using UV-vis spectrophotometer at 298 K. The single-phase region is found to increase with increase in chain length of RTIL anion. Dynamic light scattering (DLS) measurements revealed the formation of highly stable nano-sized RTIL-containing microemulsions. The size of the microemulsions increases with the addition of ionic liquid. The maximum increase in size is observed with the addition of [C2mim][C4SO4]. It is proposed that the long octyl chain of octyl sulfate allows the anion to align itself along the TX-100 molecules which increases the rigidity of microemulsions, whereas in case of [C2mim][C4SO4], the short butyl chain is apparently unable to do the same. The dynamics of solvent and rotational relaxation of coumarin 480 (C-480) has also been investigated in these ionic liquid containing microemulsions ([C2mim][C4SO4]/TX-100/cyclohexane, [C2mim][C6SO4]/TX-100/cyclohexane, and [C2mim][C8SO4]/TX-100/cyclohexane) using picosecond time-resolved fluorescence spectroscopy. In RTIL microemulsions, solvent relaxation becomes retarded compared to neat RTIL. We have also shown that with increasing R value, the solvation dynamics becomes faster and the decrease in average solvation time is more pronounced in [C2mim][C4SO4]/TX-100/cyclohexane compared to [C2mim][C6SO4]/TX-100/cyclohexane and [C2mim][C8SO4

  3. Characterization of Vadose Zone Sediments Below the TX Tank Farm: Probe Holes C3830, C3831, C3832 and 299-W10-27

    SciTech Connect

    Serne, R JEFFREY.; Bjornstad, Bruce N.; Horton, Duane G.; Lanigan, David C.; Lindenmeier, Clark W.; Lindberg, Michael J.; Clayton, Ray E.; LeGore, Virginia L.; Orr, Robert D.; Kutnyakov, Igor V.; Baum, Steven R.; Geiszler, Keith N.; Valenta, Michelle M.; Vickerman, Tanya S.

    2004-04-01

    Pacific Northwest National Laboratory performed detailed analyses on vadose zone sediments from within Waste Management Area T-TX-TY. This report contains all the geologic, geochemical, and selected physical characterization data collected on vadose zone sediment recovered from three probe holes (C3830, C3831, and C3832) in the TX Tank Farm, and from borehole 299-W-10-27. Sediments from borehole 299-W-10-27 are considered to be uncontaminated sediments that can be compared with contaminated sediments. This report also presents our interpretation of the sediment lithologies, the vertical extent of contamination, the migration potential of the contaminants, and the likely source of the contamination in the vadose zone and groundwater below the TX Tank Farm. Sediment from the probe holes was analyzed for: moisture, radionuclide and carbon contents;, one-to-one water extracts (soil pH, electrical conductivity, cation, trace metal, and anion data), and 8 M nitric acid extracts. Overall, our analyses showed that common ion exchange is a key mechanism that influences the distribution of contaminants within that portion of the vadose zone affected by tank liquor. We did not observe significant indications of caustic alteration of the sediment mineralogy or porosity, or significant zones of slightly elevated pH values in the probe holes. The sediments do show that sodium-, nitrate-, and sulfate-dominated fluids are present. The fluids are more dilute than tank fluids observed below tanks at the SX and BX Tank Farms. Three primary stratigraphic units were encountered in each probe hole: (1) backfill material, (2) the Hanford formation, and (3) the Cold Creek unit. Each of the probe holes contain thin fine-grained layers in the Hanford H2 stratigraphic unit that may impact the flow of leaked fluids and effect irregular and horizontal flow. The probe holes could not penetrate below the enriched calcium carbonate strata of the Cold Creek lower subunit; therefore, we did not

  4. Guiding Surge Reduction Strategies via Characterization of Coastal Surge Propagation and Internal Surge Generation within a Complex Bay/Estuary System, Galveston Bay, TX

    NASA Astrophysics Data System (ADS)

    Bass, B.; Torres, J.; Irza, N.; Bedient, P. B.; Dawson, C.; Proft, J.

    2015-12-01

    In this study, Hurricane Ike (2008) and a suite of synthetic storms are simulated in order to evaluate how different hurricane landfalls, wind intensities, and radius to maximum winds influence the surge response in complex semi-enclosed bays such as Galveston Bay, located along the Texas Gulf Coast. The Advanced CIRCulation and Simulating Waves Nearshore (ADCIRC+SWAN) models are employed to quantify surge in terms of its relative coastal contributions that propagate across barrier islands and tidal inlets and subsequently into Galveston Bay, the surge generated locally within the Bay itself, and the interaction between these coastal and local components of surge. Results from this research will further the current understanding of surge interactions in bay systems and guide coastal engineering surge reduction projects that need to consider multiple lines of defense to protect complex bay/estuary systems such as Galveston Bay, TX.

  5. The utility of Geographical Information Systems (GIS) in systems-oriented obesity intervention projects: The selection of comparable study sites for a quasi-experimental intervention design--TX CORD

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Texas Childhood Obesity Research Demonstration project (TX CORD) uses a systems-oriented approach to address obesity that includes individual and family interventions, community-level action, as well as environmental and policy initiatives. Given that randomization is seldom possible in communit...

  6. The effect of spider toxin PhTx3-4, ω-conotoxins MVIIA and MVIIC on glutamate uptake and on capsaicin-induced glutamate release and [Ca2+]i in spinal cord synaptosomes.

    PubMed

    Gonçaves, Jomara M; Ferreira, Juliano; Prado, Marco Antonio M; Cordeiro, Marta N; Richardson, Michael; Pinheiro, Ana Cristina do Nascimento; Silva, Marco A Romano; Junior, Celio José de Castro; Souza, Alessandra H; Gomez, Marcus Vinicius

    2011-03-01

    In spinal cord synaptosomes, the spider toxin PhTx3-4 inhibited capsaicin-stimulated release of glutamate in both calcium-dependent and -independent manners. In contrast, the conus toxins, ω-conotoxin MVIIA and xconotoxin MVIIC, only inhibited calcium-dependent glutamate release. PhTx3-4, but not ω-conotoxin MVIIA or xconotoxin MVIIC, is able to inhibit the uptake of glutamate by synaptosomes, and this inhibition in turn leads to a decrease in the Ca(2+)-independent release of glutamate. No other polypeptide toxin so far described has this effect. PhTx3-4 and ω-conotoxins MVIIC and MVIIA are blockers of voltage-dependent calcium channels, and they significantly inhibited the capsaicin-induced rise of intracellular calcium [Ca(2+)](i) in spinal cord synaptosomes, which likely reflects calcium entry through voltage-gated calcium channels. The inhibition of the calcium-independent glutamate release by PhTx3-4 suggests a potential use of the toxin to block abnormal glutamate release in pathological conditions such as pain.

  7. Analgesic Effects of GpTx-1, PF-04856264 and CNV1014802 in a Mouse Model of NaV1.7-Mediated Pain.

    PubMed

    Deuis, Jennifer R; Wingerd, Joshua S; Winter, Zoltan; Durek, Thomas; Dekan, Zoltan; Sousa, Silmara R; Zimmermann, Katharina; Hoffmann, Tali; Weidner, Christian; Nassar, Mohammed A; Alewood, Paul F; Lewis, Richard J; Vetter, Irina

    2016-03-17

    Loss-of-function mutations of Na(V)1.7 lead to congenital insensitivity to pain, a rare condition resulting in individuals who are otherwise normal except for the inability to sense pain, making pharmacological inhibition of Na(V)1.7 a promising therapeutic strategy for the treatment of pain. We characterized a novel mouse model of Na(V)1.7-mediated pain based on intraplantar injection of the scorpion toxin OD1, which is suitable for rapid in vivo profiling of Na(V)1.7 inhibitors. Intraplantar injection of OD1 caused spontaneous pain behaviors, which were reversed by co-injection with Na(V)1.7 inhibitors and significantly reduced in Na(V)1.7(-/-) mice. To validate the use of the model for profiling Na(V)1.7 inhibitors, we determined the Na(V) selectivity and tested the efficacy of the reported Na(V)1.7 inhibitors GpTx-1, PF-04856264 and CNV1014802 (raxatrigine). GpTx-1 selectively inhibited Na(V)1.7 and was effective when co-administered with OD1, but lacked efficacy when delivered systemically. PF-04856264 state-dependently and selectively inhibited Na(V)1.7 and significantly reduced OD1-induced spontaneous pain when delivered locally and systemically. CNV1014802 state-dependently, but non-selectively, inhibited Na(V) channels and was only effective in the OD1 model when delivered systemically. Our novel model of Na(V)1.7-mediated pain based on intraplantar injection of OD1 is thus suitable for the rapid in vivo characterization of the analgesic efficacy of Na(V)1.7 inhibitors.

  8. Analgesic Effects of GpTx-1, PF-04856264 and CNV1014802 in a Mouse Model of NaV1.7-Mediated Pain

    PubMed Central

    Deuis, Jennifer R.; Wingerd, Joshua S.; Winter, Zoltan; Durek, Thomas; Dekan, Zoltan; Sousa, Silmara R.; Zimmermann, Katharina; Hoffmann, Tali; Weidner, Christian; Nassar, Mohammed A.; Alewood, Paul F.; Lewis, Richard J.; Vetter, Irina

    2016-01-01

    Loss-of-function mutations of NaV1.7 lead to congenital insensitivity to pain, a rare condition resulting in individuals who are otherwise normal except for the inability to sense pain, making pharmacological inhibition of NaV1.7 a promising therapeutic strategy for the treatment of pain. We characterized a novel mouse model of NaV1.7-mediated pain based on intraplantar injection of the scorpion toxin OD1, which is suitable for rapid in vivo profiling of NaV1.7 inhibitors. Intraplantar injection of OD1 caused spontaneous pain behaviors, which were reversed by co-injection with NaV1.7 inhibitors and significantly reduced in NaV1.7−/− mice. To validate the use of the model for profiling NaV1.7 inhibitors, we determined the NaV selectivity and tested the efficacy of the reported NaV1.7 inhibitors GpTx-1, PF-04856264 and CNV1014802 (raxatrigine). GpTx-1 selectively inhibited NaV1.7 and was effective when co-administered with OD1, but lacked efficacy when delivered systemically. PF-04856264 state-dependently and selectively inhibited NaV1.7 and significantly reduced OD1-induced spontaneous pain when delivered locally and systemically. CNV1014802 state-dependently, but non-selectively, inhibited NaV channels and was only effective in the OD1 model when delivered systemically. Our novel model of NaV1.7-mediated pain based on intraplantar injection of OD1 is thus suitable for the rapid in vivo characterization of the analgesic efficacy of NaV1.7 inhibitors. PMID:26999206

  9. In-situ Measurements of Ozone Production Rates and Comparisons to Model-derived Production Rates During the Houston, TX and Denver, CO DISCOVER-AQ Campaigns

    NASA Astrophysics Data System (ADS)

    Baier, B. C.; Brune, W. H.; Miller, D. O.; Lefer, B. L.

    2015-12-01

    Tropospheric ozone (O3) is a secondary pollutant that has harmful effects on human and plant life. The climate and urban emissions in Houston, TX and Denver, CO can be conducive for significant ozone production and thus, high ozone events. Tighter government strategies for ozone mitigation have been proposed, which involve reducing the current EPA eight-hour ozone standard from 75 ppb to 65-70 ppb. These strategies rely on the reduction of ozone precursors in order to decrease the ozone production rate, P(O3). The changes in the ozone concentration at a certain location are dependent upon P(O3), so decreasing P(O3) can decrease ozone levels provided that it has not been transported from other areas. Air quality models test reduction strategies before they are implemented, locate ozone sources, and predict ozone episodes. Traditionally, P(O3) has been calculated by models. However, large uncertainties in model emissions inventories, chemical mechanisms, and meteorology can reduce confidence in this approach. A new instrument, the Measurement of Ozone Production Sensor (MOPS) directly measures P(O3) and can provide an alternate approach to determining P(O3). An updated version of the Penn State MOPS (MOPSv2.0) was deployed to Houston, TX and Denver, CO as a part of NASA's DISCOVER-AQ field campaign in the summers of 2013 and 2014, respectively. We present MOPS directly-measured P(O3) rates from these areas, as well as comparisons to zero-dimensional and three-dimensional modeled P(O3) using the RACM2 and MCMv2.2 mechanisms. These comparisons demonstrate the potential of the MOPS to test and evaluate model-derived P(O3), to advance the understanding of model chemical mechanisms, and to improve predictions of high ozone events.

  10. The Effects of Long-Range Transport of Agricultural Smoke on AOD in Houston, TX: Insights from NASA SEAC4RS and DISCOVER-AQ

    NASA Astrophysics Data System (ADS)

    Beyersdorf, A. J.; Ziemba, L. D.; Anderson, B. E.; Chen, G.; Corr, C.; Crumeyrolle, S.; Moore, R.; Thornhill, K. L., II; Winstead, E.

    2014-12-01

    In September 2013, the NASA P-3B performed systematic in-situ profiles throughout the Houston, TX area as part of the DISCOVER-AQ project. During this campaign, smoke originating from agricultural fires in the Mississippi River Valley contributed up to 80% of the aerosol optical depths (AODs) and thus complicated estimation of ground-level PM2.5 from AOD. Comparison with measurements of fresh agricultural fires during both DISCOVER-AQ and SEAC4RS showed that these lofted layers were considerably aged with higher single scattering albedos and water-uptake potential (f(RH)). This more hygroscopic aged smoke (with f(RH)'s of 1.5) created higher AODs than would be obtained if fresh smoke (f(RH) on the order of 1.1) had been present. In addition, profiling done as part of SEAC4RS throughout the Southeast will be compared to ground-based PM2.5 allowing for determination of background aerosol vertical distributions in the region and the extent of the smoke transport. Historic long-term measurements of AOD (from AERONET) and PM2.5 (from ground-based monitoring sites) will also be addressed to determine the frequency of these long-range transport events.

  11. High resolution seismic imaging at the Odessa (TX) meteorite impact site: the ground-impact geohazard and integration with magnetic gradiometry and time-domain electromagnetics

    NASA Astrophysics Data System (ADS)

    Soule, D.; Everett, M.

    2007-05-01

    Comet and asteroid bombardment along with impact crater formation has occurred continuously throughout Earth's history. The greatest impact-related geohazard is due to moderate sized impactors which represent the worst-case trade-off between frequency of occurrence, difficulty of mitigation, and severity of environmental destruction. Impacts in this size range pose a significant regional threat to densely populated areas. The primary objective of this research is to evaluate the ground-impact geohazard via high resolution seismic imaging of the remnants of a moderate-sized impact event, namely that associated with the 50 ka Odessa (TX) Group IAB iron meteorite. We provide high-resolution subsurface images of the 150-m diameter crater, the outcropping crater rim, the ejecta blanket, and the surrounding plain. The subsurface images are analyzed in terms of the deformation and thrusting of the underlying Cretaceous limestone and shale strata and the likely environmental effects caused by the impact. The analysis will build upon and extend our previous geophysical investigations based on magnetic gradiometry and time-domain electromagnetic data.

  12. Combined trisomy 9 and Ullrich-Turner syndrome in a girl with a 46,X,der(9)t(X;9)(q12;q32) karyotype.

    PubMed

    Canún, S; Mutchinick, O; Shaffer, L G; Fernández, C

    1998-11-16

    Total trisomy 9 is a rare disorder with most patients dying before age 4 months. Herein, we report a 9-year-old girl with mental retardation, short stature, a peculiar face and other minor defects, who was diagnosed as having an unbalanced de novo X-autosome translocation with a 46,X,der(9)t(X;9)(q12;q32) karyotype resulting in almost a full trisomy 9(pter-->q32) and a partial monosomy X(q12-->pter). The clinical findings of our patient, almost exclusively resemble those of trisomy 9p and the Ullrich-Turner syndromes and has few manifestations of 9q trisomy. BrdU replication studies by Giemsa staining showed an earlier replication of 9p in the translocated chromosome, but a marked late-replication pattern for almost the complete 9q arm involved in the translocation. FISH studies confirmed the presence of three 9 centromeres, excluded the presence of the X centromere signal in the rearranged chromosome, and showed that both Xq telomeric sequences were present. BrdU replication studies by FISH showed an usual pattern of striking late-replication around the XIC of the derivative chromosome, but early replication of the chromosome 9p segment and distal Xq.

  13. Studies on the micropolarities of bmimBF4/TX-100/toluene ionic liquid microemulsions and their behaviors characterized by UV-visible spectroscopy.

    PubMed

    Li, Na; Gao, Yan'an; Zheng, Liqiang; Zhang, Jin; Yu, Li; Li, Xinwei

    2007-01-30

    Ionic liquids (ILs), 1-butyl-3-methylimidazolium tetrafluoroborate (bmimBF4), were substituted for polar water and formed nonaqueous microemulsions with toluene by the aid of nonionic surfactant TX-100. The phase behavior of the ternary system was investigated, and microregions of bmimBF4-in-toluene (IL/O), bicontinuous, and toluene-in-bmimBF4 (O/IL) were identified by traditional electrical conductivity measurements. Dynamic light scattering (DLS) revealed the formation of the IL microemulsions. The micropolarities of the IL/O microemulsions were investigated by the UV-vis spectroscopy using the methyl orange (MO) and methylene blue (MB) as absorption probes. The results indicated that the polarity of the IL/O microemulsion increased only before the IL pools were formed, whereas a relatively fixed polar microenvironment was obtained in the IL pools of the microemulsions. Moreover, UV-vis spectroscopy has also shown that ionic salt compounds such as Ni(NO3)2, CoCl2, CuCl2, and biochemical reagent riboflavin could be solubilized into the IL/O microemulsion droplets, indicating that the IL/O microemulsions have potential application in the production of metallic or semiconductor nanomaterials, and in biological extractions or as solvents for enzymatic reactions. The IL/O microemulsions may have some expected effects due to the unique features of ILs and microemulsions.

  14. Amino acid sequence of TsTX-V, an alpha-toxin from Tityus serrulatus scorpion venom, and its effect on K+ permeability of beta-cells from isolated rat islets of Langerhans.

    PubMed

    Marangoni, S; Toyama, M H; Arantes, E C; Giglio, J R; da Silva, C A; Carneiro, E M; Gonçalves, A A; Oliveira, B

    1995-04-13

    Highly purified Tityustoxin V (TsTX-V), an alpha-toxin isolated from the venom of the Brazilian scorpion Tityus serrulatus, was obtained by ion exchange chromatography on carboxymethylcellulose-52. It was shown to be homogeneous by reverse phase high performance liquid chromatography, N-terminal sequencing (first 39 residues) of the reduced and alkylated protein and by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate and tricine. Following enzymatic digestion, the complete amino acid sequence (64 residues) was determined. The sequence showed higher homology with the toxins from the venoms of the North African than with those of the North and South American scorpions. Using the rate of 86Rb+ release from depolarized rat pancreatic beta-cells as a measure of K+ permeability changes, TsTX-V (5.6 micrograms/ml) was found to increase by 2.0-2.4-fold the rate of marker outflow in the presence of 8.3 mM glucose. This effect was persistent and slowly reversible, showing similarity to that induced by 100 microM veratridine, an agent that increases the open period of Na+ channels, delaying their inactivation. It is suggested that, by extending the depolarized period, TsTX-V indirectly affects beta-cell voltage-dependent K+ channels, thus increasing K+ permeability.

  15. Impact of Uncertainty in Runoff and Routing Processes on the Estimation of Non-parametric Unit Hydrographs for the Cypress Creek Watershed, TX

    NASA Astrophysics Data System (ADS)

    Soberaski, J.; Moysey, S.; Bedient, P.

    2007-05-01

    Remote sensing data and GIS tools have opened the door to simplify the parameterization of distributed watershed models. However, decisions about the spatial homogeneity of model parameters should also be based on the actual response of a basin to rainfall. For the last 75 years, hydrologists have relied on the unit hydrograph (UH) as a key tool for analyzing watersheds because its shape is directly related to important attributes of the drainage basin controlling runoff (e.g., topography, land use, soil properties, stream network, etc.). Deconvolution of excess rainfall from direct runoff can provide non-parametric estimates of the UH that capture the effects of sub-basin heterogeneity, thereby making these hydrographs particularly useful tools for comparing and classifying watersheds. Due to the mathematical instability of deconvolution, it is unclear whether meaningful UH estimates can be obtained for the purpose of inter-basin comparisons, particularly when processes controlling excess precipitation and direct runoff within the watershed are uncertain. This study evaluates the sensitivity of non-parametric UH's to uncertainty in watershed properties for six gauged sub-basins of the Cypress Creek Watershed, TX. We have used MATLAB to conduct a rainfall-runoff analysis of the Cypress Creek Watershed, TX over a 17 day period during Tropical Storm Allison in 2001. For the six basins analyzed, discharges for Cypress Creek are available at the outflow of each sub-basin and NEXRAD rainfall data are available throughout the watershed. To determine the direct runoff contributed by each sub-basin, incoming upstream flows were routed by simple advection and then subtracted from the downstream discharge record. Excess precipitation was calculated by applying the Green & Ampt infiltration model to the rainfall record for each basin after accounting for initial abstractions and direct losses due to impervious surfaces. In each step of this procedure, the parameters

  16. Clinical and pathological characteristics of Hispanic BRCA-associated breast cancers in the American-Mexican border city of El Paso, TX

    PubMed Central

    Nahleh, Zeina; Otoukesh, Salman; Dwivedi, Alok Kumar; Mallawaarachchi, Indika; Sanchez, Luis; Saldivar, J Salvador; Cataneda, Kayla; Heydarian, Rosalinda

    2015-01-01

    Hispanics in El Paso, TX, a large American-Mexican border city constitute 85% of the population. Limited cancer research has been conducted in this population. We sought to study the prevalence of BRCA mutations among Hispanic patients of Mexican origin, identify reported Mexican founder or recurrent mutations, and study the breast cancer characteristics in mutation carriers. Methods: Hispanic women of Mexican descent with a personal history of breast cancer, who presented consecutively for genetic cancer risk assessment, were enrolled in an Institutional Review Board-approved registry and underwent BRCA testing based on national guidelines. The characteristics of tumors and patients with positive BRCA mutation were analyzed. Results: 88 patients were screened; 18 patients (20%) were BRCA carriers. Among BRCA carriers, 72% were diagnosed with breast cancer at younger than 50 years, 61% had “Triple negative disease”. BRCA carriers had a significantly higher Body Mass Index (BMI) than non-carriers. Thirteen patients had BRCA1 mutations and five had BRCA2 mutations. A total of 17 deleterious BRCA Mutations were observed. Seven have been previously reported as specific genes from Mexico as country of origin. Five new mutations in BRCA carriers of Mexican descent were identified. Conclusion: Hispanic breast cancer patients of Mexican origin present at a younger age, and have predominantly triple negative tumors and high BMI. We identified 5 new mutations not reported previously in Hispanic BRCA carriers of Mexican descent. Interestingly, 41% of BRCA mutations identified have been reported as recurrent mutations in Hispanic individuals from Mexico as the country of origin. A more cost-effective approach to initial screening of Hispanic individuals based on country of origin is desirable and would potentially decrease the number of cases requiring complete sequencing. PMID:25628955

  17. Minimal phenotype in a girl with trisomy 15q due to t(X;15)(q22.3;q11.2) translocation.

    PubMed

    Stankiewicz, Paweł; Kuechler, Alma; Eller, C Daniel; Sahoo, Trilochan; Baldermann, Christiane; Lieser, Ulla; Hesse, Martin; Gläser, Christiane; Hagemann, Monika; Yatsenko, Svetlana A; Liehr, Thomas; Horsthemke, Bernhard; Claussen, Uwe; Marahrens, York; Lupski, James R; Hansmann, Ingo

    2006-03-01

    Few cases of de novo unbalanced X;autosome translocations associated with a normal or mild dysmorphic phenotype have been described. We report a 3-year-old dizygotic female twin with prenatally ascertained increased nuchal translucency. Prenatal chromosome studies revealed nearly complete trisomy 15 due to a de novo unbalanced translocation t(X;15)(q22;q11.2) confirmed postnatally. A mild phenotype was observed with normal birth measurements, minor facial dysmorphic features (hypertelorism, short broad nose, and a relatively long philtrum), and moderate developmental delay at the age of 3 years in comparison to her male fraternal twin. Replication timing utilizing BrdU and acridine-orange staining showed that the der(X) chromosome was late-replicating with variable spreading of inactivation into the translocated 15q segment. The der(X) was determined to be of paternal origin by analyses of polymorphic markers and CGG-repeat at FMR1. Methylation analysis at the SNRPN locus and analysis of microsatellites on 15q revealed paternal isodisomy with double dosage for all markers and the unmethylated SNRPN gene. The Xq breakpoint was mapped within two overlapping BAC clones RP11-575K24 and RP13-483F6 at Xq22.3 and the 15q breakpoint to 15q11.2, within overlapping clones RP11-509A17 and RP11-382A4 that are all significantly enriched for LINE-1 elements (36.6%, 43.0%, 26.6%, 22.0%, respectively). We speculate that the attenuated phenotype may be due to inactivation spreading into 15q, potentially facilitated by the enrichment of LINE-1 elements at the breakpoints. In silico analysis of breakpoint regions revealed the presence of highly identical low-copy repeats (LCRs) at both breakpoints, potentially involved in generating the translocation.

  18. Distribution and persistence of Escherichia coli and Enterococci in stream bed and bank sediments from two urban streams in Houston, TX.

    PubMed

    Brinkmeyer, Robin; Amon, Rainer M W; Schwarz, John R; Saxton, Tara; Roberts, Dustin; Harrison, Sarah; Ellis, Nicholas; Fox, Jessica; DiGuardi, Katherine; Hochman, Mona; Duan, Shuiwang; Stein, Ron; Elliott, Catherine

    2015-01-01

    The purpose of this research was to determine if Escherichia coli and enterococci in streambed and bank sediments from two urban bayous, Buffalo Bayou and White Oak Bayou, in Houston, TX, USA are a significant source of the chronically high levels of these bacteria in the overlying water. The watersheds of the bayous lie within highly urbanized areas of Greater Houston and there is primary recreational contact with the public. Extensive sampling of the watersheds was conducted from 2008 to 2010. Both fecal indicator bacteria were found at ≥ 10(4)MPNgdry wt.(-1) concentrations in the upper 1cm of sediment cores with declines by orders of magnitude at 15 and 30 cm sediment horizons and in some cases 60 cm, but, nonetheless, indicating that they can remain viable even at depth. No interannual variation was observed. And, there was no correlation with percent organic matter, however there was moderate correlation (R(2)=0.12; p=0.001) of E. coli with sediment moisture. In sediments, most E. coli and enterococci in Buffalo Bayou (76%) and White Oak Bayou (87.5%) were associated with fine sand grains (60 to 250 μm). In the water column, E. coli was associated, in roughly equal percentages, with particle sizes <10, 10-25, 25-63, and ≥ 63 μm (21.9, 25.6, 30.4, and 32.9%, respectively). Enterococci were mostly attached to particle sizes in the ranges of 10-25μm (36.0%) and 25-63 μm (31.1%) as well as ≥ 63 μm (37.7%) (p=0.0001). Fingerprinting of E. coli isolates from both bayous with Rep-PCR and the BOX A1R primer was used to demonstrate translocation of sediments from the upper to lower watersheds.

  19. 33 CFR 80.845 - Galveston, TX to Freeport, TX.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    .... (a) A line drawn from Galveston North Jetty Light 6A to Galveston South Jetty Light 5A. (b) A line formed by the centerline of the highway bridge over San Luis Pass. (c) Lines formed by the centerlines of... the seaward extremity of the Freeport North Jetty to Freeport Entrance Light 6; thence...

  20. 33 CFR 80.845 - Galveston, TX to Freeport, TX.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    .... (a) A line drawn from Galveston North Jetty Light 6A to Galveston South Jetty Light 5A. (b) A line formed by the centerline of the highway bridge over San Luis Pass. (c) Lines formed by the centerlines of... the seaward extremity of the Freeport North Jetty to Freeport Entrance Light 6; thence...

  1. The Utility of Geographical Information Systems (GIS) in Systems-Oriented Obesity Intervention Projects: The Selection of Comparable Study Sites for a Quasi-Experimental Intervention Design—TX CORD

    PubMed Central

    Byars, Allison; Byrd-Williams, Courtney; Sharma, Shreela V.; Durand, Casey; Hoelscher, Deanna M.; Butte, Nancy F.; Kelder, Steven H.

    2015-01-01

    Abstract Background: The Texas Childhood Obesity Research Demonstration project (TX CORD) uses a systems-oriented approach to address obesity that includes individual and family interventions, community-level action, as well as environmental and policy initiatives. Given that randomization is seldom possible in community-level intervention studies, TX CORD uses a quasi-experimental design. Comparable intervention and comparison study sites are needed to address internal validity bias. Methods: TX CORD was designed to be implemented in low-income, ethnically diverse communities in Austin and Houston, Texas. A three-stage Geographical Information System (GIS) methodology was used to establish and ascertain the comparability of the intervention and comparison study sites. Census tract (stage 1) and school (stage 2) data were used to identify spatially exclusive geographic areas that were comparable. In stage 3, study sites were compared on demographic characteristics, socioeconomic status (SES), food assets, and physical activity (PA) assets. Student's t-test was used to examine significant differences between the selected sites. Results: The methodology that was used resulted in the selection of catchment areas with demographic and socioeconomic characteristics that fit the target population: ethnically diverse population; lower-median household income; and lower home ownership rates. Additionally, the intervention and comparison sites were statistically comparable on demographic and SES variables, as well as food assets and PA assets. Conclusions: This GIS approach can provide researchers, program evaluators, and policy makers with useful tools for both research and practice. Area-level information that allows for robust understanding of communities can enhance analytical procedures in community health research and offer significant contributions in terms of community assessment and engagement. PMID:25587670

  2. Influence of Changing Hydrology on Pedogenic Calcite Precipitation in Vertisols, Dance Bayou, Brazoria County, Tx: Implications for Estimating Paleoatmospheric PCO2

    NASA Astrophysics Data System (ADS)

    Mintz, J. S.; Driese, S. G.; Ludvigson, G. A.; Breecker, D. O.

    2010-12-01

    ., TX.

  3. 40Gbit/s-class-λ-tunable WDM/TDM-PON using λ-selectable B-Tx and 4 x M cyclic AWG router for flexible photonic aggregation networks.

    PubMed

    Nakamura, Hirotaka; Taguchi, Katsuhisa; Tamaki, Shinya; Mizuno, Takayuki; Hashizume, Yasuaki; Yamada, Takashi; Ito, Mikitaka; Takahashi, Hiroshi; Kimura, Shunji; Yoshimoto, Naoto

    2013-01-14

    This paper proposes a 40Gbit/s-class-λ-tunable WDM/TDM-PON for flexible photonic aggregation networks that achieves the aggregation of a large number of users using the DWBA algorithm without an L2-SW. It also clarifies the scalability of the proposed system in terms of the transmission distance and the number of users. A λ-switching transmission experiment was conducted using a newly developed 10Gbit/s x 4λ selectable B-Tx and 4 x 4 cyclic AWG router.

  4. Characterization of 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([Emim][Tf2N])/TX-100/cyclohexane ternary microemulsion: Investigation of photoinduced electron transfer in this RTIL containing microemulsion

    NASA Astrophysics Data System (ADS)

    Sarkar, Souravi; Pramanik, Rajib; Ghatak, Chiranjib; Rao, Vishal Govind; Sarkar, Nilmoni

    2011-02-01

    In this study we have characterized a ionic liquid 1-ethyl-3-methylimidazolium bis(trifluoromethyl- sulfonyl)imide containing ternary nonaqueous microemulsion ([Emim][Tf2N]//TX-100/cyclo- hexane). The phase behavior and dynamic light scattering study show that the [Emim][Tf2N]/TX-100/cyclohexane three component system can form microemulsion with [Emim][Tf2N] as polar core at suitable condition. We have investigated photoinduced electron transfer (PET) using dimethyl aniline as electron donor and several Coumarin dyes as electron acceptor molecules at two different R values (R = [ionic liquid]/[surfactant]) to observe how the dynamics of the PET rate is affected in this type of confined microenvironment compared to that of the PET dynamics in neat ionic liquid and other pure solvent media. The plot of observed kq values with the free energy change (ΔG0) for electron transfer reaction shows an apparent inversion in the observed rate as predicted by the Marcus theory.

  5. Shallow groundwater monitoring at the SACROC oilfield, Scurry County, TX: good news for geologic storage of CO2 despite a complex hydrogeologic and geochemical setting (Invited)

    NASA Astrophysics Data System (ADS)

    Smyth, R. C.; Romanak, K.; Yang, C.; Hovorka, S.

    2009-12-01

    The SACROC water study is the first comprehensive research project with application to geologic storage (GS) of CO2 that focuses on collection and interpretation of field measurements of groundwater (water level and water chemistry data). CO2 has been injected for enhanced oil recovery at the SACROC oilfield in Scurry County, TX since 1972. Hence, we have a perfect natural laboratory and an analog for monitoring future commercial CO2 sequestration sites. Kinder Morgan currently operates the SACROC oilfield where over 150 million metric tons (MMT) of CO2 has been injected for EOR at ~2 km depth; over 75 MMT of the CO2 has been produced and re-injected. CO2 is assumed to be trapped in the deep subsurface at SACROC. The goals of monitoring shallow groundwater over CO2 injection sites are to (1) confirm that CO2 has remained in the deep subsurface and (2) assess impacts to water quality if CO2 were to migrate upward along conduit flow paths (e.g. leaking well bores). We collected groundwater and stratigraphic data within an ~3,000 km2 area centered on SACROC to establish regional variability prior to assessing potential impacts to groundwater from CO2 injection. Groundwater data include results from five sampling trips between June 2006 and November 2008, and a compilation of historical data from the Texas Water Development Board database, dating back to 1936. Sources of complexity that contribute to data interpretation challenges include: (1) regional historic oilfield activity, (2) multiple freshwater-bearing strata in the regional Dockum aquifer, (3) sampled wells screened in shallowest (30 m), deepest (150 m), or across both water-bearing zones, (4) variable discharge rate of sampled wells (<5 to >250 gpm), (5) groundwater flow divide that bisects SACROC, (6) variable aquifer recharge mechanisms, (7) temporal variability in groundwater levels and chemistry, (8) cation exchange, (9) presence of biogenically-produced CO2 in aquifer, and (10) incongruent dissolution

  6. Interaction of ionic liquid with water with variation of water content in 1-butyl-3-methyl-imidazolium hexafluorophosphate ([bmim][PF6])/TX-100/water ternary microemulsions monitored by solvent and rotational relaxation of coumarin 153 and coumarin 490.

    PubMed

    Seth, Debabrata; Chakraborty, Anjan; Setua, Palash; Sarkar, Nilmoni

    2007-06-14

    The interaction of water with room temperature ionic liquid (RTIL) [bmim][PF6] has been studied in [bmim][PF6]/TX-100/water ternary microemulsions by solvent and rotational relaxation of coumarin 153 (C-153) and coumarin 490 (C-490). The rotational relaxation and average solvation time of C-153 and C-490 gradually decrease with increase in water content of the microemulsions. The gradual increase in the size of the microemulsion with increase in w0 (w0=[water]/[surfactant]) is evident from dynamic light scattering measurements. Consequently the mobility of the water molecules also increases. In comparison to pure water the retardation of solvation time in the RTIL containing ternary microemulsions is very less. The authors have also reported the solvation time of C-490 in neat [bmim][PF6]. The solvation time of C-490 in neat [bmim][PF6] is bimodal with time constants of 400 ps and 1.10 ns.

  7. Interaction of ionic liquid with water with variation of water content in 1-butyl-3-methyl-imidazolium hexafluorophosphate ([bmim][PF6])/TX-100/water ternary microemulsions monitored by solvent and rotational relaxation of coumarin 153 and coumarin 490

    NASA Astrophysics Data System (ADS)

    Seth, Debabrata; Chakraborty, Anjan; Setua, Palash; Sarkar, Nilmoni

    2007-06-01

    The interaction of water with room temperature ionic liquid (RTIL) [bmim][PF6] has been studied in [bmim][PF6]/TX-100/water ternary microemulsions by solvent and rotational relaxation of coumarin 153 (C-153) and coumarin 490 (C-490). The rotational relaxation and average solvation time of C-153 and C-490 gradually decrease with increase in water content of the microemulsions. The gradual increase in the size of the microemulsion with increase in w0 (w0=[water]/[surfactant]) is evident from dynamic light scattering measurements. Consequently the mobility of the water molecules also increases. In comparison to pure water the retardation of solvation time in the RTIL containing ternary microemulsions is very less. The authors have also reported the solvation time of C-490 in neat [bmim][PF6]. The solvation time of C-490 in neat [bmim][PF6] is bimodal with time constants of 400ps and 1.10ns.

  8. Detection of the synovial sarcoma translocation t(X;18) (SYT;SSX) in paraffin-embedded tissues using reverse transcriptase-polymerase chain reaction: a reliable and powerful diagnostic tool for pathologists. A molecular analysis of 221 mesenchymal tumors fixed in different fixatives.

    PubMed

    Guillou, L; Coindre, J; Gallagher, G; Terrier, P; Gebhard, S; de Saint Aubain Somerhausen, N; Michels, J; Jundt, G; Vince, D R; Collin, F; Trassard, M; Le Doussal, V; Benhattar, J

    2001-01-01

    Synovial sarcoma (SS) is a relatively rare sarcoma, which may be confused with several other mesenchymal and nonmesenchymal lesions. It bears the t(X;18) (SYT;SSX) translocation, which seems to be specific for this tumor type and can be detected in paraffin-embedded tissue, using reverse transcriptase-polymerase chain reaction (RT-PCR). However, the specificity and sensitivity of this detection method have rarely been examined in a large series. Using RT-PCR, we examined 250 mesenchymal and nonmesenchymal, benign and malignant, paraffin-embedded lesions for the SS t(X;18) (SYT-SSX) translocation. PCR products were obtained from 221 tumors (88.5%). There were 135 non-SS tumors, 22 biphasic, and 64 monophasic spindle/round cell SS, of which 10 were cytogenetically confirmed as t(X;18)-positive. SYT-SSX gene fusion transcripts were detected in the SS tumor category only (100% specificity), including 100% of the biphasic SS and 86% of monophasic spindle/round cell SS. Nine tumors originally diagnosed as SS were t(X;18) (SYT-SSX)-negative. Following reassessment, only 3 of these tumors showed clinicopathologic, immunohistochemical, and/or ultrastructural features consistent with that diagnosis, thus raising the overall detection sensitivity to 96%. With regard to the potential adverse effect of the fixatives used, PCR products were obtained in 100%, 91.5%, 90.5%, and 0% of tumors fixed with AFA, buffered formalin, Holland Bouin, and conventional Bouin's fluid, respectively. This study shows that the detection of the SS t(X;18) (SYT-SSX) in paraffin-embedded tissue is feasible with a 100% specificity and an overall 96% sensitivity, provided non-Bouin's fluid fixation is used.

  9. Incorporating Primary and Secondary Prevention Approaches To Address Childhood Obesity Prevention and Treatment in a Low-Income, Ethnically Diverse Population: Study Design and Demographic Data from the Texas Childhood Obesity Research Demonstration (TX CORD) Study

    PubMed Central

    Butte, Nancy F.; Barlow, Sarah; Vandewater, Elizabeth A.; Sharma, Shreela V.; Huang, Terry; Finkelstein, Eric; Pont, Stephen; Sacher, Paul; Byrd-Williams, Courtney; Oluyomi, Abiodun O.; Durand, Casey; Li, Linlin; Kelder, Steven H.

    2015-01-01

    Abstract Background: There is consensus that development and evaluation of a systems-oriented approach for child obesity prevention and treatment that includes both primary and secondary prevention efforts is needed. This article describes the study design and baseline data from the Texas Childhood Obesity Research Demonstration (TX CORD) project, which addresses child obesity among low-income, ethnically diverse overweight and obese children, ages 2–12 years; a two-tiered systems-oriented approach is hypothesized to reduce BMI z-scores, compared to primary prevention alone. Methods: Our study aims are to: (1) implement and evaluate a primary obesity prevention program; (2) implement and evaluate efficacy of a 12-month family-centered secondary obesity prevention program embedded within primary prevention; and (3) quantify the incremental cost-effectiveness of the secondary prevention program. Baseline demographic and behavioral data for the primary prevention community areas are presented. Results: Baseline data from preschool centers, elementary schools, and clinics indicate that most demographic variables are similar between intervention and comparison communities. Most families are low income (≤$25,000) and Hispanic/Latino (73.3–83.8%). The majority of parents were born outside of the United States. Child obesity rates exceed national values, ranging from 19.0% in preschool to 35.2% in fifth-grade children. Most parents report that their children consume sugary beverages, have a television in the bedroom, and do not consume adequate amounts of fruits and vegetables. Conclusions: Interventions to address childhood obesity are warranted in low-income, ethnically diverse communities. Integrating primary and secondary approaches is anticipated to provide sufficient exposure that will lead to significant decreases in childhood obesity. PMID:25555188

  10. Severe Weather Forecasting for Laughlin AFB, TX

    DTIC Science & Technology

    2007-03-01

    showers is assumed to be of weaker intensity, while DMC that culminates in thunderstorms is assumed to be of stronger intensity.” Deep moist convection...FL, Amer. Meteor . Soc., 547-550. ______, R. E. Jewell, and H. E. Brooks, 2002: Comparison between observed convective cloud base heights and...11th Conf. on Severe Local Storms, Kansas City, MO, Amer. Meteor . Soc., 397-402. 80 _____, F. Caracena and M. Magnano, 1985: Temporal evolution of

  11. 49 CFR 372.203 - Beaumont, TX.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... commerce, not under common control, management, or arrangement for a continuous carriage or shipment to or... combined areas defined in paragraphs (b) and (c) of this section, and (e) All of any municipality...

  12. Teacher Professional Development in Laredo, TX

    NASA Astrophysics Data System (ADS)

    Finkelstein, Keely D.; Macri, L. M.; Hemenway, M.; Wetzel, M.; Preston, S.; Rood, M.

    2014-01-01

    In the fall of 2012, McDonald Observatory, Texas A&M University, and Texas A&M International University conducted a series of workshops on astronomy content for 5th - 8th grade teachers in Laredo, Texas. Three one-day workshops were held at the Lamar Bruni Vergara Planetarium of Texas A&M International University, using a mix of in-person and distance learning technology. Texas A&M professor Lucas Macri gave public talks in English and Spanish, and a lunch-time presentation to the teachers. A series of evaluation tools were used to assess the success of the workshops. A Nominal Group Technique (NGT) discussion was used, through which groups developed consensus answers about their learning, expectations for classroom use, and satisfaction with the workshop. The Astronomy/Space Science Test (MOSART Grades 5-8) was also issued as a pre- and post-test to assess gains in knowledge. Teacher consensus was that the materials and activities of the workshop had been helpful for learning and that they expected to use many of them in their classrooms. However, the evaluation also showed that teachers would have preferred the Observatory educator be physically present for all workshops. Past video-conferencing workshops, where local facilitators first participated in workshops at the Observatory, showed better feedback and results concerning this point. Comparing those results to the present case, we conclude that more clearly defined roles and better training for the science specialists and local facilitators would improve the video conference experience for the teachers. Comparison of pre- and post-test results showed improved teacher knowledge. An additional benefit of this project was the further development of partnerships between McDonald Observatory and Texas A&M International University, which has resulted in further education projects, including a video-conference presentation series to eight-grade students and their families. This secondary project focused on motivating and increasing underserved students from the Laredo area in STEM fields, and featured lectures from University of Texas / McDonald Observatory astronomers.

  13. TX18: Cognitive Workload and Fatigue Study

    DTIC Science & Technology

    2012-09-01

    both orbital fossa (HEOG). BioSemi Respiration Belt: The belt measures changes in thoracic or abdominal circumference during respiration through...of computer operation (e.g., eye strain, neck strain, headache, etc.) and symptoms commonly associated with motion sickness. All participants will

  14. 75 FR 70763 - Texas Disaster #TX-00363

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-18

    ... is a notice of an Administrative declaration of a disaster for the State of Texas dated 11/09/2010. Incident: Tropical Storm Hermine. Incident Period: 09/06/2010 through 09/10/2010. Effective Date: 11/09/2010. Physical Loan Application Deadline Date: 01/10/2011. Economic Injury (EIDL) Loan...

  15. History of model development at Temple, TX

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Model development at Temple, Texas, USA has a long history. Prior to the actual model develop-ment research, a hydrological data collection programme was established at Riesel, Texas (about 60 km northeast of Temple) in 1937. Data collected from the Riesel watersheds during 1937–2006 have been valua...

  16. Micro-Grids for Colonias (TX)

    SciTech Connect

    Dean Schneider; Michael Martin; Renee Berry; Charles Moyer

    2012-07-31

    This report describes the results of the final implementation and testing of a hybrid micro-grid system designed for off-grid applications in underserved Colonias along the Texas/Mexico border. The project is a federally funded follow-on to a project funded by the Texas State Energy Conservation Office in 2007 that developed and demonstrated initial prototype hybrid generation systems consisting of a proprietary energy storage technology, high efficiency charging and inverting systems, photovoltaic cells, a wind turbine, and bio-diesel generators. This combination of technologies provided continuous power to dwellings that are not grid connected, with a significant savings in fuel by allowing power generation at highly efficient operating conditions. The objective of this project was to complete development of the prototype systems and to finalize and engineering design; to install and operate the systems in the intended environment, and to evaluate the technical and economic effectiveness of the systems. The objectives of this project were met. This report documents the final design that was achieved and includes the engineering design documents for the system. The system operated as designed, with the system availability limited by maintenance requirements of the diesel gensets. Overall, the system achieved a 96% availability over the operation of the three deployed systems. Capital costs of the systems were dependent upon both the size of the generation system and the scope of the distribution grid, but, in this instance, the systems averaged $0.72/kWh delivered. This cost would decrease significantly as utilization of the system increased. The system with the highest utilization achieved a capitol cost amortized value of $0.34/kWh produced. The average amortized fuel and maintenance cost was $0.48/kWh which was dependent upon the amount of maintenance required by the diesel generator. Economically, the system is difficult to justify as an alternative to grid power. However, the operational costs are reasonable if grid power is unavailable, e.g. in a remote area or in a disaster recovery situation. In fact, avoided fuel costs for the smaller of the systems in use during this project would have a payback of the capital costs of that system in 2.3 years, far short of the effective system life.

  17. Fluid Substitution Modeling to Determine Sensitivity of 3D Vertical Seismic Profile Data to Injected CO­2­ at an active Carbon Capture, Utilization and Storage Project, Farnsworth field, TX.

    NASA Astrophysics Data System (ADS)

    Haar, K. K.; Balch, R. S.

    2015-12-01

    The Southwest Regional Partnership on Carbon Sequestration monitors a CO2 capture, utilization and storage project at Farnsworth field, TX. The reservoir interval is a Morrowan age fluvial sand deposited in an incised valley. The sands are between 10 to 25m thick and located about 2800m below the surface. Primary oil recovery began in 1958 and by the late 1960's secondary recovery through waterflooding was underway. In 2009, Chaparral Energy began tertiary recovery using 100% anthropogenic CO2 sourced from an ethanol and a fertilizer plant. This constitutes carbon sequestration and fulfills the DOE's initiative to determine the best approach to permanent carbon storage. One purpose of the study is to understand CO­2 migration from injection wells. CO2­ plume spatial distribution for this project is analyzed with the use of time-lapse 3D vertical seismic profiles centered on CO2 injection wells. They monitor raypaths traveling in a single direction compared to surface seismic surveys with raypaths traveling in both directions. 3D VSP surveys can image up to 1.5km away from the well of interest, exceeding regulatory requirements for maximum plume extent by a factor of two. To optimize the timing of repeat VSP acquisition, the sensitivity of the 3D VSP surveys to CO2 injection was analyzed to determine at what injection volumes a seismic response to the injected CO­2 will be observable. Static geologic models were generated for pre-CO2 and post-CO2 reservoir states through construction of fine scale seismic based geologic models, which were then history matched via flow simulations. These generated static states of the model, where CO2­ replaces oil and brine in pore spaces, allow for generation of impedance volumes which when convolved with a representative wavelet generate synthetic seismic volumes used in the sensitivity analysis. Funding for the project is provided by DOE's National Energy Technology Laboratory (NETL) under Award No. DE-FC26-05NT42591.

  18. 76 FR 43933 - Radio Broadcasting Services; Markham, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-22

    ..., adopted June 27, 2011, and released June 28, 2011. The full text of this Commission decision is available..., Portals II, 445 12th Street, SW., Room CY-A257, Washington, DC 20554. The complete text of this...

  19. 75 FR 80013 - Radio Broadcasting Services; Jewett, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-21

    .... 10-65, RM-10595, adopted December 1, 2010, and released December 3, 2010. The full text of this... Information Center, Portals II, 445 12th Street, SW., Room CY-A257, Washington, DC 20554. The complete text...

  20. Evolving Postmortems as Teams Evolve Through TxP

    DTIC Science & Technology

    2014-12-01

    NAVAIR),Code 414300D,STOP 6308, 1900 N. Knox Road,China Lake ,CA,93555-6106 8. PERFORMING ORGANIZATION REPORT NUMBER 9. SPONSORING/MONITORING AGENCY...and instructor for NAVAIR at China Lake , California, where he coaches engineering teams in the development of high qual- ity aviation products for...NAVAIR Code 414300D STOP 6308, 1900 N. Knox Road China Lake , CA 93555-6106 Phone: 760-939-0666 E-mail: bradley.hodgins@navy.mil 1. NAVAIR is the

  1. 75 FR 63533 - Environmental Impact Statement: Cameron County, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-15

    ... proposed improvements to United States Highway 181/State Highway 286 (Crosstown Expressway), in Nueces... Nueces County, Texas. The project limits were defined as the limits of the schematic design. The project... the Corpus Christi Ship Channel but south of the Nueces Bay Causeway; the southern limit was the...

  2. 77 FR 44142 - Drawbridge Operation Regulation; Neches River, Beaumont, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-27

    ... lift span bridge across the Neches River, mile 19.5, at Beaumont, Texas. The deviation is necessary to... span bridge across the Neches River at mile 19.5 in Beaumont, Texas. The vertical clearance of the... span of the bridge is automated and normally not manned but will open on signal for the passage...

  3. 76 FR 26606 - Drawbridge Operation Regulation; Neches River, Beaumont, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-09

    ... the KCS vertical lift span bridge across the Neches River, mile 19.5, at Beaumont, Texas. The... operating schedule of the vertical lift span bridge across the Neches River at mile 19.5 in Beaumont, Texas... 117.971, the vertical lift span of the bridge is automated and normally not manned but will open...

  4. 77 FR 25112 - Radio Broadcasting Services; Centerville and Midway, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-27

    ... distance separation requirements of the Rules with a site restriction 7.6 kilometers (4.7 miles) northwest... separation requirement of the Rules with a site restriction 10.6 kilometers (6.6 miles) northwest...

  5. 77 FR 58799 - Radio Broadcasting Services; Roaring Springs, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-24

    ... Springs, Texas, in compliance with the Commission's minimum distance separation requirements with a site... minimum distance separation requirements with a site restriction of 9.4 km (5.8 miles) northeast...

  6. Valero Hydrocarbons BACT Analysis, PSD-TX-746

    EPA Pesticide Factsheets

    This document may be of assistance in applying the New Source Review (NSR) air permitting regulations including the Prevention of Significant Deterioration (PSD) requirements. This document is part of the NSR Policy and Guidance Database. Some documents in the database are a scanned or retyped version of a paper photocopy of the original. Although we have taken considerable effort to quality assure the documents, some may contain typographical errors. Contact the office that issued the document if you need a copy of the original.

  7. High Performance Builder Spotlight: GreenCraft, Lewisville, TX

    SciTech Connect

    2011-01-01

    In October and November 2009, the TimberCreek Zero Energy House in Lewisville, Texas, opened as a Building America Demonstration House. The 2,538-foot,three-bedroom, 2½-bath custom-built home showed a home energy rating score (HERS) of 56 without the solar photovoltaics and a HERS score of 1 with PV.

  8. 76 FR 73503 - Amendment of Class E Airspace; Winters, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-29

    ... (817) 321- 7716. SUPPLEMENTARY INFORMATION: History On August 26, 2011, the FAA published in the... Worth, Texas, on November 9, 2011. Gail L. Kasson, Acting Manager, Operations Support Group, ATO...

  9. 75 FR 18941 - Environmental Impact Statement: Bexar County, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-13

    ... proposed improvements to Loop 1604 in San Antonio, Texas, within Bexar County limits to enhance mobility... changes to the aforementioned EIS for proposed Loop 1604 improvements. Specifically, the southwestern..., advising the public that they would be preparing an EIS for transportation improvements to Loop 1604...

  10. 77 FR 7228 - Texas Disaster Number TX-00382

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-10

    ... Only for the State of Texas (FEMA- 4029-DR), dated 09/21/2011. Incident: Wildfires. ] Incident Period: 08/30/2011 through 12/31/2011. Effective Date: 02/01/2012. Physical Loan Application Deadline Date: 11/21/2011. Economic Injury (EIDL) Loan Application Deadline Date: 06/21/2012. ADDRESSES:...

  11. 77 FR 25075 - Special Local Regulation; Galveston Bay, Kemah, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-27

    ... local regulation is necessary to provide for the safety of swimmers participating in the Memorial... is needed to protect swimmers participating in the Memorial Hermann Kemah Triathlon. Basis and Purpose The swimming portion of the Memorial Hermann Triathlon will feature swimmers entering the...

  12. 76 FR 36613 - Environmental Impact Statement; Nueces County, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-22

    ... facility that meets current highway design standards. A Feasibility Study completed in 2003 evaluated four..., as well as the No-build alternative. The Feasibility Study resulted in the identification of a... Feasibility Study, will be identified and evaluated in the EIS, in addition to the No-build Alternative,...

  13. PCB-Caulk Replacement Project Johnson Space Center Houston, TX

    NASA Technical Reports Server (NTRS)

    Young, William M.; Stanch, Penney M.; Molenda, William

    2011-01-01

    Wet method reduced exposure by minimizing overall respirable particulate release. Dry method didn't introduce delays for primer/caulk application. Removed caulks came in many forms, from dry powdery to tarry sticky. Varying textures were not sampled or packaged differently. During the course of the project, EPA modified recommended practices to include full containment for exterior caulk removal. Changes are ongoing. Initial recommendations were directed to school buildings. EPA is researching risks due to caulk. Exposure guidance lacking except for 2 of 209 PCB congeners. Work was safely completed on schedule and under budget.

  14. 76 FR 61775 - Texas Disaster Number TX-00381

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-05

    ...: (Physical Damage and Economic Injury Loans): Harrison, Smith, Upshur. Contiguous Counties: (Economic Injury... declaration remains unchanged. (Catalog of Federal Domestic Assistance Numbers 59002 and 59008) Joseph...

  15. Instrument Corrosion During Sterilization at USAF Hospital, Dyess AFB, TX

    DTIC Science & Technology

    1975-05-01

    0.1 0.2 ɘ.1 0.2 Chloride 76 20 320 12 8 Fluorides 1.6 0.5 2.2 0.3 ɘ.1 Nitrates ə ə Not Run ə ə Phosphates (total) 0.2 ɘ.2 .50 ɘ.2 ɘ.2 Sulfates ...Ammonia 0 Nitrite 0.09 Nitrate 2.32 Sulfate 25 Chloride 64 75 Calcium 14 20 Iron ɘ.02 Sodium 38 Magnessium 15 Potassium 6 Phosphate ɘ.2 Total Solids...IRON SULFIDES, AND HARDNESS MAGIESIUM AND CALCUIM ). 1. CARRYOVER OF WATER WITH THE STEAM MAY BE CONTRIBUTING TO THE PROBLEM. ;ECAUSE OF THE COMPLEXITY OF

  16. Evolving Postmortems as Teams Evolve Through TxP

    DTIC Science & Technology

    2014-11-01

    Process Resource Team – a PI History at NAVAIR PSP classes TSP Launches TPI Research TPI Launches Process Modeling 2011 2010 2009 2008...plan all work from first launch forward – Work is based on all products and services defined in process modeling – PSP for Engineers training planned...Modeling (one to four half-day sessions) Plan the work (four days) Work the plan (cycle 1) (three to nine months) PSP Fundamentals (one week

  17. Information Engineering of the curricular Officers’ Segment of a Unified Student Academic Database System for NPS

    DTIC Science & Technology

    1991-09-01

    HOUSING OCCUPANT SECTION-NUMBER SPLIT SECTION PROPOSED NPS DEGREE ACCREDITATION STATUS NPS MAJOR COMMISSIONING SOURCE STUDY SPACE LOCKER NUMBER DATE OF...ACCREDITATION STATUS NPS MAJOR - COMMISSIONING- SOURCE STUDY SPACE LOCKER NUMBER DATE OF BIRTH PLACE OF BIRTH CITY PLACE OF BIRTH STATE DATE REPORTED ABOARD...LAMESA HOUSING OCCUPANT SECTION -NUMBER SPLIT SECTION - PROPOSED NPS DEGREE ACCREDITATION STATUS NPS MAJOR - COMMISSIONING- SOURCE STUDY SPACE LOCKER

  18. The Mystery of Historical Channel Shoaling at Houston-Galveston Navigation Channel, TX

    DTIC Science & Technology

    2011-05-01

    Trinity Bay GALVESTON BAY GULF OF MEXICO Bolivar Roads Big Reef Red Fish Reef Atkinson Island Houston Fig. 1. HGNC location map and definition...Other activities in the bay that may have altered channel shoaling include oyster dredging and shrimp trawling, which artificially suspend sediment in

  19. 76 FR 16294 - Drawbridge Operation Regulation; Buffalo Bayou, Mile 4.3, Houston, Harris County, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-23

    ... under section 4(a) of the Administrative Procedure Act (APA) (5 U.S.C. 553(b)). This provision... Property Rights. Civil Justice Reform This rule meets applicable standards in sections 3(a) and 3(b)(2) of... under Executive Order 13211. Technical Standards The National Technology Transfer and Advancement...

  20. 78 FR 12419 - Blacklands Railroad, Inc.-Discontinuance Exemption-In Rusk County, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-22

    ... Rusk Cnty., Tex., Docket No. AB 1103X. \\2\\ Blacklands was granted authority to lease and operate the... refer to Docket No. AB 1108X and must be sent to: (1) Surface Transportation Board, 395 E Street...

  1. Finding No Significant Impact: Aircraft Weather Shelter at Laughlin AFB, TX

    DTIC Science & Technology

    2004-06-01

    Fiscal year Gallons per minute Hazardous materials Hazardous Materials Management Plan Headquarters Hazardous and Solid Waste Amendments Heating...defined by the Solid Waste Disposal Act as amended by the Hazardous and Solid waste Amendments (HSW A). In general, both hazardous materials and wastes

  2. Precipitation-centered Conceptual Model for Sub-humid Uplands in Lampasas Cut Plains, TX

    NASA Astrophysics Data System (ADS)

    Potter, S. R.; Tu, M.; Wilcox, B. P.

    2011-12-01

    Conceptual understandings of dominant hydrological processes, system interactions and feedbacks, and external forcings operating within catchments often defy simple definition and explanation, especially catchments encompassing transition zones, degraded landscapes, rapid development, and where climate forcings exhibit large variations across time and space. However, it is precisely those areas for which understanding and knowledge are most needed to innovate sustainable management strategies and counter past management blunders and failed restoration efforts. The cut plain of central Texas is one such area. Complex geographic and climatic factors lead to spatially and temporally variable precipitation having frequent dry periods interrupted by intense high-volume precipitation. Fort Hood, an army post located in the southeast cut plain contains landscapes ranging from highly degraded to nearly pristine with a topography mainly comprised of flat-topped mesas separated by broad u-shaped valleys. To understand the hydrology of the area and responses to wet-dry cycles we analyzed 4-years of streamflow and rainfall from 8 catchments, sized between 1819 and 16,000 ha. Since aquifer recharge/discharge and surface stream-groundwater interactions are unimportant, we hypothesized a simple conceptual model driven by precipitation and radiative forcings and having stormflow, baseflow, ET, and two hypothetical storage components. The key storage component was conceptualized as a buffer that was highly integrated with the ET component and exerted controls on baseflow. Radiative energy controlled flux from the buffer to ET. We used the conceptual model in making a bimonthly hydrologic budget, which included buffer volumes and a deficit-surplus indicator. Through the analysis, we were led to speculate that buffer capacity plays key roles in these landscapes and even relatively minor changes in capacity, due to soil compaction for example, might lead to ecological shifts. The model led us to other hypotheses concerning stormflow mechanisms and controls on baseflow, which we then tested against observations. It was instructive that such a simple model could lead to interesting new theories.

  3. 75 FR 16514 - Maxim Integrated Products, Formerly Known as Dallas Semiconductor, Dallas, TX; Amended...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-01

    ... certification for workers of the subject firm. The workers produced integrated circuits (analog and mixed signal... shift in production of integrated circuits (analog and mixed ] signal) to Japan, Thailand and the... Employment and Training Administration Maxim Integrated Products, Formerly Known as Dallas...

  4. Fracture mechanics; Proceedings of the Nineteenth National Symposium, San Antonio, TX, June 30-July 2, 1986

    SciTech Connect

    Cruse, T.A.

    1988-01-01

    The papers contained in this volume provide an overview of current theoretical and experimental research in the field of fracture mechanics. Topics discussed include three-dimensional issues, computational and analytical issues, damage tolerance and fatigue, elastoplastic fracture, dynamic inelastic fracture, and crack arrest theory and applications. Papers are presented on approximate methods for analysis of dynamic crack growth and arrest, constraint-loss model for the growth of surface fatigue cracks, fatigue crack growth in aircraft main landing gear wheels, and near-threshold crack growth in nickel-base superalloys.

  5. Cloud-to-Ground Lightning Characteristics Over Houston, TX: 1989-2000

    NASA Astrophysics Data System (ADS)

    Steiger, S. M.; Orville, R. E.

    2001-12-01

    Cloud-to-Ground (CG) lightning detected by the National Lightning Detection Network (NLDN) indicates a relatively high flash density over Houston, Texas for the twelve-year period 1989-2000. A significant enhancement of 45% in the flash density is observed compared to the nearby surrounding areas. The strength of the enhancement varies based on season and time-of-day, with the greatest increases occurring during the summer (58%), and during the 0900-1800 LST time periods in each season. Observations indicate that large lightning events (defined as days with > 1,000 flashes in a geographic region that includes Houston and nearby rural areas) were responsible for the climatological lightning anomaly, and that increased thunderstorm initiation was not the most significant cause of the enhancement. A decrease (-12%) in the percentage of positive flashes is observed over the city. Higher negative median peak currents along the coast and well into the Gulf of Mexico were also discovered. Several explanations for our observations are suggested. The urban heat island and increased cloud condensation nuclei (CCN) concentrations, especially from industrial pollution, are shown to be significant factors in creating lightning enhancement. Pollution effects are speculated to cause a change in a thunderstorm's charge distribution, which can affect the polarity of CG flashes. The potential effect of the nearby coastal Gulf salt water on the measured peak current is examined.

  6. Forensic Hydrological Investigation of the Blanco River Flood May 2015, Wimberley, TX

    NASA Astrophysics Data System (ADS)

    Furl, C.

    2015-12-01

    A forensic hydrological investigation of a major flash flood was conducted for the Blanco River in south-central Texas. The unprecedented flood occurred during the early morning hours of May 24th leaving 12 dead in the towns of Wimberley and San Marcos. Hundreds of homes were damaged or destroyed, two reinforced concrete bridges were washed off their piers, and nearly 100 high water rescues were made the following day. The present work characterizes the meteorological setup leading to the event, describes the flood hydrology using the Gridded Surface Subsurface Hydrologic Analysis (GSSHA) model, and reports on an extensive field campaign seeking to document high water marks throughout the 1200 km2 basin. Results indicate high precipitable water values, large CAPE, and strong mid and upper level winds aided in impressive divergence over the region. This allowed for storms to continually produce heavy rainfall over the same areas. Large regions of the catchment received greater than 200 mm across the upper portion of the basin with 24 hr maximums around 330 mm. GSSHA simulations indicate good performance when compared to a stage hydrograph recorded mid-catchment. The remaining USGS gauges failed early on during the rising limb of the hydrograph. Model estimates indicate peak streamflow was approximately 5500 cms with stage values nearing 13 m as the flood wave moved through the town of Wimberley. Approximately 125 locations were examined for high water marks along the mainstem of the river using RTK GPS. Stage values ranged from 12 - 18 m.

  7. 77 FR 65011 - Buffalo Lake National Wildlife Refuge, Randall County, TX; Comprehensive Conservation Plan and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-24

    ... human encroachment on the refuge. ] Wind Energy Research Conduct a diurnal avian Same as Alternative A, Same as Alternative B. activity survey and plus actively track acoustic monitoring in wind energy order... habitat for migratory waterfowl and grassland bird species. Background The CCP Process The...

  8. Geochemical Indicators of Urban Development in Tributaries and Springs along the Bull Creek Watershed, Austin, TX

    NASA Astrophysics Data System (ADS)

    Senison, J. J.; Banner, J. L.; Reyes, D.; Sharp, J. M.

    2012-12-01

    Urbanization can cause significant changes to both flow and water quality in streams and tributaries. In the Austin, Texas, area, previous studies have demonstrated that streamwater strontium isotope compositions (87Sr/86Sr) correlate with measures of urbanization when comparing non-urbanized streams to their urban counterparts. The inclusion of municipal water into natural surface water is inferred from the mean 87Sr/86Sr value found in urbanized streams, which falls between the high value in treated municipal water and the lower values found in local surface streams sourcing from non-urbanized catchments. Fluoride is added to municipal tap water in the treatment process, and a correlation between 87Sr/86Sr and fluoride is observed in streamwater sampled from the watersheds around Austin. These relationships represent some of the principal findings reported in Christian et al. (2011). Current research is testing the hypothesis that municipal water influx in urban areas is a primary modifier of stream- and spring-water chemistry in a single watershed that contains a strong gradient in land use. We compare 87Sr/86Sr and other chemical constituents with potential contributing endmembers, such as municipal tap water and wastewater, local soil and rock leachates, and land use within the Bull Creek watershed. As a consequence of the history of land development, some Bull Creek tributaries are sourced and flow almost entirely in fully-developed areas, whereas others are located in protected natural areas. Thirteen tributaries were monitored and classified as either urbanized or non-urbanized based upon land use within the tributary catchment. Springs in the Bull Creek watershed were also sampled and are similarly classified. The Bull Creek watershed is composed of Lower Cretaceous limestone with significantly lower 87Sr/86Sr than that of municipal water taken from the Lower Colorado River, which is underlain in part by Precambrian rocks upstream of Austin. There are notable differences in urbanized vs. non-urbanized watersheds in mean concentrations of fluoride (urbanized: 0.27 ± 0.08 vs. non-urbanized: 0.19 ± 0.01 ppm), sodium (34.7 ± 17.3 vs. 8.4 ± 1.0 ppm), and potassium (2.9 ± 0.8 vs. 1.2 ± 0.2 ppm), consistent with higher concentrations in municipal water contributing to the urbanized tributaries. Springwater demonstrates similar divergences for these ions. 87Sr/86Sr for the springs falls within a narrow range for non-urbanized springs (0.7079-0.7081), similar to Cretaceous limestone values, whereas urbanized springs contain a larger range (0.7077-0.7087). These results are consistent with urbanization effects in the Bull Creek watershed.

  9. AEROSOL COMPOSITION AND TRENDS AT LA PORTE, TX DURING TEXAQS 2000. (R826240)

    EPA Science Inventory

    The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

  10. 75 FR 52016 - Notice of Inventory Completion: Texas Parks and Wildlife Department, Austin, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-24

    ... Kegley, archeologist, Texas Parks and Wildlife Department. The human remains were inventoried as Burials.... Based on the burial context and their association with House 2 at Hueco Tanks Village, archeological..., archeologist, Texas Parks and Wildlife Department. The human remains were inventoried as ``Burial 5.'' No...

  11. Upper Strawn (Desmoinesian) carbonate and clastic depositional environments, SE King County, TX

    SciTech Connect

    Boring, T.H. )

    1990-05-01

    The Pennsylvanian upper Strawn Group of southeast King County, Texas, provides a unique setting to study interactions between coeval carbonate and clastic deposition during the Desmoinesian. One of the most perplexing problems is the relationship of massive Pennsylvanian platform carbonates to shallow-water marine and deltaic sediments. Within the study area carbonate facies were deposited along the northern edge of the Knox-Baylor trough on the Spur platform, and terrigenous clastics were carried toward the Midland basin through the Knox-Baylor trough. Based on the analysis of subsurface cores, five carbonate lithofacies and four clastic lithofacies were recognized in southeast King County, Texas. The distribution and geometry of these lithofacies are related to variations in the rate of subsidence in the Knox-Baylor trough, Pennsylvanian tectonics, deltaic progradation, avulsion and compaction. The platform carbonates within the northern region record environments within the carbonate platform complex, including middle platform, outer platform, algal mound, and platform margin. The quartzarenitic sandstones within the southern region occur in a variety of complex depositional geometries, including distributary bar fingers, lobate deltas, and offshore bars. The upper Strawn Group provides an attractive area for exploration geology. Both carbonates and clastics provide excellent reservoirs from a depth of approximately 5,000 to 6,000 ft. Total production since the early 1940s, within the area is over 100,000,000 bbl of oil. Multiple pay zones within a 600-ft interval also provide an added incentive for exploration. Areas within and around the Knox-Baylor trough deserve additional study due to these relatively shallow, unexplored, multiple pay zones.

  12. Lunar and Planetary Science Conference, 20th, Houston, TX, Mar. 13-17, 1989, Proceedings

    SciTech Connect

    Sharpton, V.L.; Ryder, G.

    1990-01-01

    Topics discussed include the petrology and geochemistry of the moon, the geology of the moon, lunar regolith processes and resources, the petrology and geochemistry of achondrites, comets and interplanetary dust, shock and terrestrial cratering, the geology of Mars, and the geology of Venus. Papers are presented on silicate liquid immiscibility in isothermal crystallization experiments; highly evolved and ultramafic lithologies from Apollo 14 soils; the relationship between orbital, earth-based, and sample data for lunar landing sites; and the volcanotectonic evolution of Mare Frigoris. Attention is also given to glass variants and multiple HASP trends in Apollo 14 regolith breccias, the characterization of lunar ilmenite resources, the U-Th-Pb systematics of the Estherville mesosiderite, and the extraterrestrial halogen and sulfur contents of the stratosphere. Other papers are on argon-40/argon-39 dating of impact craters; the outliers of dust along the southern margin of the Tharsis region, Mars; and the geology of southern Guinevere Planitia, Venus, based on analyses of Goldstone radar data.

  13. 78 FR 15683 - Approval of Subzone Status, Expeditors International of Washington, Inc.; El Paso, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-12

    ... Foreign-Trade Zones Board Approval of Subzone Status, Expeditors International of Washington, Inc.; El... application submitted by the City of El Paso, grantee of FTZ 68, requesting subzone status subject to the existing activation limit of FTZ 68, on behalf of Expeditors International of Washington, Inc., in El...

  14. 3D Characterization of the Magnetic Signature of a Medium Sized Impact Crater at Odessa, TX

    NASA Astrophysics Data System (ADS)

    Robinson, A.; Soule, D.; Everett, M.; Rodman, T.; Mangue Ndong, M.; Pereira, A.; Platt, P.; Trahan, A.

    2008-12-01

    Meteorite impacts are a common occurrence throughout Earth's geologic history. Many of the surface expressions of large ancient impacts have been subsequently erased by weathering and erosion processes. The study of preserved meteorite impacts is necessary to better understand this natural hazard which has been increasingly linked to rapid climate change and mass extinctions. The 60 ka Odessa meteorite crater located in Ector Co. Texas, is unique because it is not only well-preserved, but also has been the subject of extensive geologic examination. Geologic mapping and numeric models indicate that the crater was caused by a relatively small oblique impactor. The crater rim is remarkably well exposed. Much of the ejecta blanket is present, although deeply eroded. There has been considerable site disturbance due to drilling, shaft excavation, trenching, construction of a museum, trails, and the oil/gas activity in surrounding fields. Two previous geophysical investigations have shown that our data clearly corresponds to large-scale thrust deformation. With this in mind we have performed 3D high resolution magnetic gradiometer surveys that will allow us to quantify and characterize the magnetic signature of small to medium impacts. We will tie this data set to a 3D photorealistic outcrop image provided by laser scanning with coarser-scale, below-ground geophysical information. Our geophysical imagery provides a useful constraint on numerical simulations of the impact and its immediate regional-scale environmental effects. This information can be used to identify impact sites whose surface expression has been erased by natural erosional processes, allowing for improved frequency estimates and improved geo-hazard assessment.

  15. Fugitive greenhouse gas emissions from shale gas activities - a case study of Dish, TX

    NASA Astrophysics Data System (ADS)

    Khan, A.; Roscoe, B.; Lary, D.; Schaefer, D.; Tao, L.; Sun, K.; Brian, A.; DiGangi, J.; Miller, D. J.; Zondlo, M. A.

    2012-12-01

    We evaluate new findings on aerial (horizontal and vertical) mapping of methane emissions in the atmospheric boundary layer region to help study fugitive methane emissions from extraction, transmission, and storage of natural gas and oil in Dish, Texas. Dish is located in the Barnett Shale which has seen explosive development of hydraulic fracking activities in recent years. The aerial measurements were performed with a new laser-based methane sensor developed specifically for an unmanned aerial vehicle (UAV). The vertical cavity surface emitting laser (VCSEL) methane sensor, with a mass of 2.5 kg and a precision of < 20 ppbv methane at 1 Hz, was flown on the UT-Dallas ARC Payload Master electronic aircraft at two sites in Texas: one representative of urban emissions of the Dallas-Fort Worth area in Richardson, Texas and another in Dish, Texas, closer to gas and oil activities. Methane mixing ratios at Dish were ubiquitously in the 3.5 - 4 ppmv range which was 1.5 - 2 ppmv higher than methane levels immediately downwind of Dallas. During the flight measurements at Dish, narrow methane plumes exceeding 20 ppmv were frequently observed at altitudes from the surface to 130 m above the ground. Based on the wind speed at the sampling location, the horizontal widths of large methane plumes were of the order of 100 m. The locations of the large methane plumes were variable in space and time over a ~ 1 km2 area sampled from the UAV. Spatial mapping over larger scales (10 km) by ground-based measurements showed similar methane levels as the UAV measurements. To corroborate our measurements, alkane and other hydrocarbon mixing ratios from an on-site TCEQ environmental monitoring station were analyzed and correlated with methane measurements to fingerprint the methane source. We show that fugitive methane emissions at Dish are a significant cause of the large and ubiquitous methane levels on the 1-10 km scale.

  16. 75 FR 6872 - Aransas National Wildlife Refuge Complex, Aransas, Calhoun, and Refugio Counties, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-12

    ... Fish and Wildlife Service Aransas National Wildlife Refuge Complex, Aransas, Calhoun, and Refugio... (CCP) and an environmental assessment (EA) for the Aransas National Wildlife Refuge Complex (NWRC... preferred alternative, to manage this Refuge complex for the 15 years following approval of the final...

  17. 75 FR 54420 - Cancellation of Supplemental Environmental Impact Statement (SEIS); Travis County, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-07

    ... Statement (SEIS) would be prepared for the proposed SH 71 roadway between Riverside Drive and SH 130 in... for proposed improvements to State Highway (SH) 71 from Riverside Drive to SH 130, in Travis County... to 40 CFR 1508.22 and 43 TAC Sec. 2.5(e)(2) for proposed improvement of SH 71 from Riverside Drive...

  18. 2013 INORGANIC REACTION MECHANISMS GORDON RESEARCH CONFERENCE (MARCH 3-8, 2013 - HOTEL GALVEZ, GALVESTON TX)

    SciTech Connect

    Abu-Omar, Mahdi M.

    2012-12-08

    The 2013 Gordon Conference on Inorganic Reaction Mechanisms will present cutting-edge research on the molecular aspects of inorganic reactions involving elements from throughout the periodic table and state-of-the art techniques that are used in the elucidation of reaction mechanisms. The Conference will feature a wide range of topics, such as homogeneous and heterogeneous catalysis, metallobiochemistry, electron-transfer in energy reactions, polymerization, nitrogen fixation, green chemistry, oxidation, solar conversion, alkane functionalization, organotransition metal chemistry, and computational chemistry. The talks will cover themes of current interest including energy, materials, and bioinorganic chemistry. Sections cover: Electron-Transfer in Energy Reactions; Catalytic Polymerization and Oxidation Chemistry; Kinetics and Spectroscopy of Heterogeneous Catalysts; Metal-Organic Chemistry and its Application in Synthesis; Green Energy Conversion;Organometallic Chemistry and Activation of Small Molecules; Advances in Kinetics Modeling and Green Chemistry; Metals in Biology and Disease; Frontiers in Catalytic Bond Activation and Cleavage.

  19. Environmental Assessment for North Central Texas Relay Node Site NO. RN8C915TX

    DTIC Science & Technology

    1993-02-11

    River drainage basins (Davenport, 1990). The central and southern portions of the SSA drain southward into the Brazos River (Jones, 1990; Sims, 1989...guy anchors and to prevent inadvertent exposure to electric shock resulting from the buildup of static electric charge. In addition to the main tower...area in Knox and Baylor counties centered 3.6 miles south of the town of Vera, in the Southern Great Plains portion of the Central Lowlands

  20. Campaign supports new name for TX hospital. Effort expands children's hospital's image and increases awareness.

    PubMed

    2007-01-01

    For years, the North Texas Hospital for Children at Medical City in Dallas struggled with its brand awareness. It's long-winded name was largely unknown among the city's 1.2 million residents. The hospital needed a new name and it needed one fast. The year 2005 proved to be both a burden and an opportunity for the 311-bed pediatric hospital. It survived a legal battle with a local competitor for the right to use the word "children's" in its name, created a new identity, and launched a three-year branding initiative to introduce its new name: Medical City Children's Hospital.

  1. Environmental Assessment for Multiple Projects at Laughlin Air Forc Base, TX

    DTIC Science & Technology

    2013-02-06

    Environmental Quality (CEQ) regulations for implementing the procedural provisions of the National Environmental Policy Act (NEP A) of 1969, as amended, 40...accordance with the National Environmental Policy Act, the Council on Environmental Quality regulations and implementing regulations set forth in 32 CFR 989...1969, as amended (42 United 16 States Code [U.S.C.] 4321 6 et seq.); the Council on Environmental Quality (CEQ) Regulations for 17 Implementing

  2. 75 FR 48411 - Noise Compatibility Program Notice; Fort Worth Alliance Airport, Fort Worth, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-10

    ... Federal Aviation Administration Noise Compatibility Program Notice; Fort Worth Alliance Airport, Fort... Fort Worth Alliance Airport under the provisions of 49 U.S.C. 47501 et seq. (the Aviation Safety and... exposure maps submitted under 14 CFR Part 150 for Fort Worth Alliance Airport were in compliance...

  3. 75 FR 78799 - Noise Compatibility Program Notice, Fort Worth Alliance Airport, Fort Worth, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-16

    ... Federal Aviation Administration Noise Compatibility Program Notice, Fort Worth Alliance Airport, Fort... 5, 2009. On December 1, 2010, the FAA approved the Fort Worth Alliance Airport noise compatibility... Date: The effective date of the FAA's approval of the Fort Worth Alliance Airport noise...

  4. 33 CFR 165.813 - Security Zones; Ports of Houston and Galveston, TX.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., Texas, moving security zones are established encompassing all waters within 500 yards of a cruise ship... transit of the cruise ship and continues while the cruise ship is moored or anchored. (b) Regulations. (1... enter within 500 yards but not closer than 100 yards of a cruise ship provided they operate at...

  5. 33 CFR 165.813 - Security Zones; Ports of Houston and Galveston, TX.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., Texas, moving security zones are established encompassing all waters within 500 yards of a cruise ship... transit of the cruise ship and continues while the cruise ship is moored or anchored. (b) Regulations. (1... enter within 500 yards but not closer than 100 yards of a cruise ship provided they operate at...

  6. 33 CFR 165.813 - Security Zones; Ports of Houston and Galveston, TX.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., Texas, moving security zones are established encompassing all waters within 500 yards of a cruise ship... transit of the cruise ship and continues while the cruise ship is moored or anchored. (b) Regulations. (1... enter within 500 yards but not closer than 100 yards of a cruise ship provided they operate at...

  7. 33 CFR 165.813 - Security Zones; Ports of Houston and Galveston, TX.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., Texas, moving security zones are established encompassing all waters within 500 yards of a cruise ship... transit of the cruise ship and continues while the cruise ship is moored or anchored. (b) Regulations. (1... enter within 500 yards but not closer than 100 yards of a cruise ship provided they operate at...

  8. 33 CFR 165.813 - Security Zones; Ports of Houston and Galveston, TX.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., Texas, moving security zones are established encompassing all waters within 500 yards of a cruise ship... transit of the cruise ship and continues while the cruise ship is moored or anchored. (b) Regulations. (1... enter within 500 yards but not closer than 100 yards of a cruise ship provided they operate at...

  9. 76 FR 43339 - Hunt Fee at Caddo Lake National Wildlife Refuge, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-20

    ... to curtail this population growth. The hunting of feral hogs has become a popular sport in the State... population dynamics throughout the state, TPWD has identified 33 unique Resource Management Units (RMUs... population data from identified RMUs and associated ecoregions throughout the state and adjusts deer...

  10. Energy Optimization Assessment at U.S. Army Installations: Fort Bliss, TX

    DTIC Science & Technology

    2008-09-01

    Research Laboratory CFL compact fluorescent lamp CFR Code of the Federal Regulations COP coefficient of performance DB dry bulb DDC direct digital...8,399 1,218 0.1 LI_3E CF7: CFL 23 integral unit ELC 116 33,996 7 $3,199 104 $2,879 $6,111 $12,189 $1,978 0.2 LI_3D CF5: CFL 18 integral unit ELC...LI_3F CF9: CFL 26 integral unit ELC 830 243,249 65 $23,704 –132 –$1,109 $3,729 $26,324 $16,937 0.6 LI_4U EX11: Exit - electroluminescent panel 275

  11. 78 FR 48293 - Amendment of Class D and Class E Airspace; San Marcos, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-08

    ... from the 4.2-mile radius of the airport, and small segments of Class E airspace extend 13.1 miles west, 11.1 miles northwest, 10.4 miles both east and south, and 9.6 miles southeast of the 6.7-mile radius... upward from the surface to and including 3,100 feet MSL within a 4.2-mile radius of San Marcos...

  12. 33 CFR 165.808 - Corpus Christi Ship Channel, Corpus Christi, TX, safety zone.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Petroleum Gas, the waters within a 500 yard radius of the LPG carrier while the vessel transits the Corpus... within a 500 yard radius of the LPG carrier while the vessel departs the LPG facility and transits...

  13. The influence of coastal wetlands on hurricane surge in Corpus Christi, TX

    NASA Astrophysics Data System (ADS)

    Ferreira, C.; Irish, J. L.; Olivera, F.

    2010-12-01

    The State of Texas has historically faced hurricane-related damage episodes, with Ike being the most recent example. It is expected that, in the future, hurricanes will intensify due to climate change causing greater surges, while the attenuating effect of wetlands on storm surges will also be modified due to sea level rise changes in wetland vegetation type and spatial location. Numerical analysis of storm surges is an important instrument to predict and simulate flooding extent and magnitude in coastal areas. Most operational surge models account for the influence of wetlands and other vegetation by momentum loss due to friction at the bottom and by reduction of imposed wind stress. A coupled hydrodynamic model (ADCIRC) and wave model (SWAN) was employed, and wetlands were characterized using Manning’s n, surface canopy, and surface roughness. The wetlands parameters were developed from: 1) the National Land Cover Dataset (NLCD) 1992 and 2001; 2) the National Wetlands Inventory (NWI) 2001. The calibrated coupled model for two historical hurricanes, Bret and Beulah, was used to simulate the storm surge for each scenario. Preliminary results for the sensitivity analyses, for hurricane Bret, comparing the scenarios with parameters developed from NLCD and NWI datasets with four hypothetical scenarios considering very high and low Manning’s n and wind stress (surface canopy) values showed that, for areas inside Nueces Bay, the storm surge high could vary up to four times depending on the parameter selection, for areas inside Corpus Christi Bay, the storm surge high varied around three times and behind the barrier island the storm surge high variation was less than three times. This study is a first step for an evaluation of the impact that sea level rise, climate changed wetlands, wetlands restoration, land use change, and wetlands degradation have on hurricane related surge elevation and extent in the city of Corpus Christi.

  14. Lunar and Planetary Science Conference, 18th, Houston, TX, Mar. 16-20, 1987, Proceedings

    NASA Technical Reports Server (NTRS)

    Ryder, Graham (Editor)

    1988-01-01

    Papers on lunar and planetary science are presented, including petrogenesis and chemistry of lunar samples, geology and petrogenesis of the Apollo 15 landing site, lunar geology and applications, cratering records and cratering effects, differentiated meteorites, chondritic meteorites and asteroids, extraterrestrial grains, Venus, Mars, and icy satellites. The importance of lunar granite and KREEP in very high potassium basalt petrogenesis, indentifying parent plutonic rocks from lunar breccia and soil fragments, glasses in ancient and young Apollo 16 regolith breccias, the formation of the Imbrium basin, the chemistry and petrology of the Apennine Front, lunar mare ridges, studies of Rima Mozart, electromagnetic energy applications in lunar resource mining and construction, detecting a periodic signal in the terrestrial cratering record, and a search for water on the moon, are among the topics discussed. Other topics include the bidirectional reflectance properties of Fe-Ni meteorites, the nature and origin of C-rich ordinary chondrites and chondritic clasts, the dehydration kinetics of shocked serpentine, characteristics of Greenland Fe/Ni cosmic grains, electron microscopy of a hydrated interplanetary dust particle, trapping Ne, Ar, Kr, and Xe in Si2O3 smokes, gossans on Mars, and a model of the porous structure of icy satellites.

  15. 76 FR 76298 - Drawbridge Operation Regulation; Old Brazos River, Freeport, Brazoria County, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-07

    ... operation of the Union Pacific Railroad Swing Span Bridge across the Old Brazos River, mile 4.4, at Freeport... feet above mean sea level (MSL). This deviation will allow the lift span of the bridge to remain in the... (7a-09-8) dated September 14, 2010. The project involves the replacement of the old swing span...

  16. 76 FR 14052 - Notice of Inventory Completion: Sabine River Authority of Texas, Quitman, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-15

    ... fragments. Dr. Harrell Gill-King, Anthropologist, University of North Texas, performed an examination of the human and non-human remains at the request of the Hunt County Sheriff's Department. Dr....

  17. Elliott, C. D. (2007). "Differential Ability Scales" (2nd Ed.). San Antonio, TX: Harcourt Assessment

    ERIC Educational Resources Information Center

    Beran, Tanya N.

    2007-01-01

    "Differential Ability Scales," Second Edition (DAS-II; Elliott, 2007), was developed to measure the cognitive abilities of children and adolescents from age 2 years, 6 months to 17 years, 11 months. The test is designed to be individually administered, measuring general conceptual and reasoning ability ("g") as well as specific…

  18. 78 FR 8492 - Foreign-Trade Zone 84-Houston, TX Application for Expansion

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-06

    ..., Houston Ship Channel,16398 Jacintoport Blvd.; Site 8 (162 acres)-- Central Green Business Park, 16638 Air..., and Battleground Business Park (10 acres), located at the corner of Porter Road and Old Underwood Road, La Porte; Site 18 (106 acres, sunset 5/31/2014)--Bay Area Business Park, located at Red Bluff...

  19. Women's wellness club a trusted communications vehicle. Methodist Healthcare system, San Antonio, TX.

    PubMed

    Moore, P

    1997-01-01

    Methodist Healthcare System's long-standing WomanPlus program came in handy when the system announced its new partnership with Columbia/HCA. Already faithful consumers got the news through already established mediums.

  20. 78 FR 20451 - Drawbridge Operation Regulations; Pelican Island Causeway, Galveston, Channel, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-05

    ... the island through the Galveston Channel, an additional distance of up to three miles. B. Basis and Purpose The Pelican Island Causeway bridge, Galveston Channel mile 4.5 (GIWW mile 356.1), at Galveston... with 33 CFR 117.966, the draw of the Pelican Island Causeway bridge across Galveston Channel, mile...

  1. 33 CFR 165.827 - Regulated Navigation Area; Galveston Channel, TX.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Regulated Navigation Area... HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY REGULATED NAVIGATION AREAS AND LIMITED ACCESS AREAS Specific Regulated Navigation Areas and Limited Access Areas Eighth Coast Guard District §...

  2. 33 CFR 165.827 - Regulated Navigation Area; Galveston Channel, TX.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Regulated Navigation Area... HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY REGULATED NAVIGATION AREAS AND LIMITED ACCESS AREAS Specific Regulated Navigation Areas and Limited Access Areas Eighth Coast Guard District §...

  3. 77 FR 64541 - Trinity River National Wildlife Refuge, Liberty County, TX; Final Comprehensive Conservation Plan...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-22

    ... to Refuge office. 5. Climate Change Plant trees to Same as Alternative A, Same as Alternative B... butterfly garden, and construct fishing unit; construct full- parking, and portable pier at McGuire...

  4. Best Practices Case Study: David Weekley Homes - Eagle Springs and Waterhaven, Houston, TX

    SciTech Connect

    none,

    2011-04-01

    Case study describing David Weekley Homes, Houston Division, has qualified more than 1,240 homes for the DOE Builders Challenge. Advanced framed 2x6 walls with open headers and two-stud corners allow more room for R-20 damp sprayed cellulose wall cavity insulation that is covered with R-5 rigid XPS foam. A radiant barrier cuts heat gain in the R-38 insulated vented attics. Draft stopping at fireplace and duct chases and behind tubs, gluing sheetrock to framing, and extensive caulking make for air-tight homes at 3.0 ACH50.

  5. Best Practices Case Study: Imagine Homes - Stillwater Ranch, San Antonio, TX

    SciTech Connect

    none,

    2011-04-01

    This case study describes Imagine Homes, who met Builders Challenge criteria on more than 200 homes in San Antonio with rigid foam exterior sheathing, ducts and air handler in conditioned space in a spray-foam insulated attic, and high-efficiency HVAC, windows, and appliances.

  6. 78 FR 25226 - Proposed Amendment of Class E Airspace; Harlingen, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-30

    ... regulatory, aeronautical, economic, environmental, and energy-related aspects of the proposal. Communications... database. Class E airspace areas are published in Paragraph 6005 of FAA Order 7400.9W, dated August 8,...

  7. 77 FR 58354 - Foreign-Trade Zone 265-Conroe, TX; Notification of Proposed Production Activity, Bauer...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-20

    ... components, pneumatic cylinders, flexible tubing, diesel engines and related parts, hydraulic engines/motors... extinguishers, hydraulic jacks/hoists, parts of boring/sinking machines, controllers,...

  8. A W-Band Heterodyne FMCW Radar Based on TX IQ-Modulation

    NASA Astrophysics Data System (ADS)

    Feger, Reinhard; Wagner, Christoph; Stelzer, Andreas

    2011-08-01

    In this paper a method to realize a heterodyne frequency-modulated continuous-wave (FMCW) radar is presented. The proposed principle relies on the use of an inphase/quadrature (IQ)-modulator as single-sideband-mixer to shift the frequency of the FMCW-output away from dc. Since in any IQ-modulator phase and amplitude imbalances occur, the effect of these imbalances will be investigated by deriving the corresponding signal model. Based on this analysis, a method to compensate the imbalances by a predistortion of the modulation signal will be derived. The presented method does not require special test signals or additional hardware, but relies on a standard FMCW measurement onto a calibration target. A prototype radar system was built and used in test measurements to verify the proposed principle. The measurement results show that a suppression of unwanted signal components caused by IQ-imbalances better than 45 dB is achievable.

  9. 76 FR 9639 - Prevailing Rate Systems; Redefinition of the Shreveport, LA; Texarkana, TX; Milwaukee, WI; and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-22

    ... employees, Reporting and recordkeeping requirements, Wages. U.S. Office of Personnel Management. John Berry...: Cherokee Gregg Harrison Panola Rusk Upshur * * * * * TEXAS * * * * * Texarkana Survey Area Texas:...

  10. EPAs Energy Star Names Odessa, TX, as Nations Top Small City for Energy Efficient Buildings

    EPA Pesticide Factsheets

    DALLAS - (March 26, 2015) The U.S. Environmental Protection Agency (EPA) has announced Odessa, Texas, as the nation's leader among smaller cities for Energy Star certified buildings. Odessa boasts 31 buildings with the certification-all schools and

  11. Sugarcane aphid resistant sorghums found within USDA-ARS Lubbock, TX genotypes

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The sugarcane aphid, Melanaphis sacchari, was discovered infesting grain sorghum in the southern United States in 2013 and has been a perennial pest through 2016. We evaluated 25 sorghum genotypes in a search for host plant resistance to this pest. Plants were started from seed (N = 16 replication...

  12. 78 FR 26056 - Waterway Suitability Assessment for Expansion of Liquefied Gas Terminals; Nederland, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-03

    ... SECURITY Coast Guard Waterway Suitability Assessment for Expansion of Liquefied Gas Terminals; Nederland... Coast Guard regulations, Sunoco Partners Marketing and Terminals has submitted a Letter of Intent and a... new construction to expand or modify marine terminal operations in an existing facility...

  13. 78 FR 4356 - Proposed Modification of the Dallas/Fort Worth Class B Airspace Area; TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-22

    ... from the Dallas/Fort Worth International Airport (DFW) and Dallas Love Field Airport (DAL) within Class... ] and from the Dallas/Fort Worth International Airport and Dallas Love Field Airport within Class...

  14. Abstracts of the Annual Meeting of Planetary Geologic Mappers, San Antonio, TX, 2009

    NASA Technical Reports Server (NTRS)

    Bleamaster, Leslie F., III (Editor); Tanaka, Kenneth L.; Kelley, Michael S.

    2009-01-01

    Topics covered include: Geologic Mapping of the Beta-Atla-Themis (BAT) Region of Venus: A Progress Report; Geologic Map of the Snegurochka Planitia Quadrangle (V-1): Implications for Tectonic and Volcanic History of the North Polar Region of Venus; Preliminary Geological Map of the Fortuna Tessera (V-2) Quadrangle, Venus; Geological Map of the Fredegonde (V-57) Quadrangle, Venus; Geological Mapping of the Lada Terra (V-56) Quadrangle, Venus; Geologic Mapping of V-19; Lunar Geologic Mapping: A Preliminary Map of a Portion of the LQ-10 ("Marius") Quadrangle; Geologic Mapping of the Lunar South Pole, Quadrangle LQ-30: Volcanic History and Stratigraphy of Schr dinger Basin; Geologic Mapping along the Arabia Terra Dichotomy Boundary: Mawrth Vallis and Nili Fossae, Mars; Geologic Mapping Investigations of the Northwest Rim of Hellas Basin, Mars; Geologic Mapping of the Meridiani Region of Mars; Geology of a Portion of the Martian Highlands: MTMs -20002, -20007, -25002 and -25007; Geologic Mapping of Holden Crater and the Uzboi-Ladon-Morava Outflow System; Mapping Tyrrhena Patera and Hesperia Planum, Mars; Geologic Mapping of Athabaca Valles; Geologic Mapping of MTM -30247, -35247 and -40247 Quadrangles, Reull Vallis Region, Mars Topography of the Martian Impact Crater Tooting; Mars Structural and Stratigraphic Mapping along the Coprates Rise; Geology of Libya Montes and the Interbasin Plains of Northern Tyrrhena Terra, Mars: Project Introduction and First Year Work Plan; Geology of the Southern Utopia Planitia Highland-Lowland Boundary Plain: Second Year Results and Third Year Plan; Mars Global Geologic Mapping: About Half Way Done; New Geologic Map of the Scandia Region of Mars; Geologic Mapping of the Medusae Fossae Formation on Mars and the Northern Lowland Plains of Venus; Volcanism on Io: Insights from Global Geologic Mapping; and Planetary Geologic Mapping Handbook - 2009.

  15. 75 FR 54695 - Noise Exposure Map Notice; Brownsville South Padre Island International Airport, Brownsville, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-08

    ... noise exposure contours depicted on a noise exposure map submitted under section 47503 of the Act, it... properties with regard to the depicted noise contours, or in interpreting the noise exposure maps to resolve... noise exposure contours onto the map depicting properties on the surface rests exclusively with...

  16. 75 FR 74073 - Laguna Atascosa National Wildlife Refuge, Cameron and Willacy Counties, TX; Final Comprehensive...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-30

    ... wetlands, estuarine wetlands, lomas (clay ridges), wind tidal flats, and barrier island beaches and ] dunes... strategies to address future establishment of artificial water sources. Selected Alternative After... species, and provide/enhance brush land, wetland and grassland habitat for ocelots, migratory...

  17. 77 FR 54891 - Reorganization of Foreign-Trade Zone 149 Under Alternative Site Framework Freeport, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-06

    ... Border Protection port of entry, FTZ 149's existing Sites 1, 3 and 10 would be categorized as magnet... provision for magnet sites that would terminate authority for Sites 3 and 10 if not activated by August...

  18. 77 FR 54890 - Reorganization of Foreign-Trade Zone 94 Under Alternative Site Framework; Laredo, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-06

    ... existing Sites 1 through 7 would be categorized as magnet sites, and FTZ 94's existing Sites 8 through 11... limit for the zone, to a five-year ASF sunset provision for magnet sites that would terminate...

  19. 77 FR 44582 - Reorganization of Foreign-Trade Zone 183 Under Alternative Site Framework; Austin, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-30

    ..., and FTZ 183's existing Sites 1 through 24 would be categorized as magnet sites; Whereas, notice... project, and to a five-year ASF sunset provision for magnet sites that would terminate authority for...

  20. 77 FR 31651 - Texas Gamma Ray, LLC, Pasadena, TX; Confirmatory Order (Effective Immediately)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-29

    ... culture, willfulness, security of licensed material, and ethics. Within 60 days of the date of the... material, and ethics. b. Within 60 days of the date of the Confirmatory Order, TGR will contract with an... Internet, or in some cases to mail copies on electronic storage media. Participants may not submit...

  1. Proceedings of Lunar and Planetary Science, Volume 22; Conference, Houston, TX, Mar. 18-22, 1991

    NASA Technical Reports Server (NTRS)

    Ryder, Graham (Editor); Sharpton, Virgil L. (Editor)

    1992-01-01

    Various papers on lunar and planetary science are presented. Individual topics addressed include: analysis of Phobos Mission Gamma ray spectra from Mars, comparison of volcanic and modified landforms from Tharsis Montes on Mars, polygenetic origin of Hrad Vallis region of Mars, new evidence of lacustrine basins on Mars, flood surge through the Lunae Planum Outflow Complex on Mars, interpretation of canyon materials and flood sources on Kasei Valles on Mars, geochemistry of Manson Impact structure rocks, micrometer-sized glass spheres in Apollo 16 soil 61181, isotopic abundances in Pesyanoe of solar-type xenon, mineralogy of 12 large 'chondritic' interplanetary dust particles. Also discussed are: trace elements in chondritic stratospheric particles, evolution of isotopic signatures in lunar regolith nitrogen, pyroclastic deposits on the western limb of the moon, origin of picritic green glass magmas by polybaric fractional fusion, origin of yellow glasses associated with Apollo 15 KREEP basalt fragments, trace elements in 59 mostly highland moon rocks, mineralization on the moon, relation between diogenite cumulates and eucrite magmas.

  2. 76 FR 9640 - Prevailing Rate Systems: Santa Clara, CA, Tulsa County, OK, and Angelina County, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-22

    ... MANAGEMENT 5 CFR Part 532 RIN 3206-AM22 Prevailing Rate Systems: Santa Clara, CA, Tulsa County, OK, and... Schedule of Nonappropriated Fund Regular Wage Schedules by removing, under the State of California, ``Santa..., ``Santa Clara,'' which was abolished as a NAF FWS wage area by a final rule (74 FR 9951) published...

  3. 78 FR 48842 - Proposed Establishment of Class D Airspace; Mesquite, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-12

    ... control tower at Mesquite Metro Airport ] has made this action necessary for the safety and management of Instrument Flight Rules (IFR) operations at Mesquite Metro Airport. DATES: Comments must be received on or... airspace from the surface up to but not including 2,000 feet MSL within a 3.5-mile radius of Mesquite...

  4. Documentation for release of a high oleic runner peanut cultivar (tested as TX 994313)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Although the peanut acreage diminished in 2008 to 18,000 acres, the peanut crop remains a viable agricultural commodity in western and southwestern Oklahoma with an annual estimated value of $14 million. Production of peanut is adversely affected by soilborne pathogens, especially Sclerotinia minor...

  5. The Effect of Prescribed Burns and Wildfire on Vegetation in Bastrop State Park, TX

    NASA Astrophysics Data System (ADS)

    Justice, C. J.

    2014-12-01

    In 2011, central Texas had its worst drought since the 1950's. This, in conjunction with the strong winds produced by Tropical Storm Lee created conditions that made possible the Bastrop County Complex Fire in September 2011. These record-breaking wildfires burned over 95% of the 6,565-acre Bastrop State Park (BSP). Since 2003, BSP had been using prescribed burns as a management practice to reduce fuel load and prevent high severity wildfires. Although these prescribed fires did not prevent the 2011 wildfires they may have mitigated their effects. This study considered the effect of prescribed burn history and wildfire burn severity on vegetation recovery in BSP since the 2011 wildfire. The hypotheses of this study are that prescribed burn history and wildfire burn severity separately and jointly have affected post wildfire vegetation. To test these hypotheses, data were collected in 2013 from 46 plots across BSP using the Fire Effects Monitoring and Inventory (FIREMON) protocol to determine herbaceous plant density, shrub density, overstory density, and midstory tree density. Data were analyzed using analyses of variance (ANOVA) to determine the effects of prescribed fire and wildfire severity on these vegetation measurements. It was found that more severely burned plots had more herbaceous plants, fewer midstory trees, and lower shrub densities than less severely burned plots. Contrary to an initial hypotheses, there were few relationships between prescribed burn history and wildfire effects. The only significant effect detected for prescribed burning was the positive effect of prescribed fire on midstory tree density, but only for plots that were not severely burned in the wildfire. In this system, burn severity had a greater effect on post-wildfire vegetation than prescribed burns.

  6. The predicted impact of increased formaldehyde emissions from industrial flares on ozone concentrations in Houston, TX.

    NASA Astrophysics Data System (ADS)

    Wang, C. T.; Vizuete, W.

    2015-12-01

    Houston features one of the largest concentrations of the petrochemical industry in all of North America and flares are widely used there as the final treatment process for unwanted volatile organic compounds. These flares have the potential to produce formaldehyde as the result of incomplete combustion. Formaldehyde emissions are an important precursor to producing hydroxyl radicals and thus can impact atmospheric chemistry and the formation of ozone. Formaldehyde emissions from flares, however, are difficult to measure in situ. Recently, alternative measurement techniques have been developed, like open path optical methods, that allow the direct measurement of flare emissions from the facility's fence line (Johansson et al., 2014; Pikelnaya, Flynn, Tsai, & Stutz, 2013). This observational data indicates that the emission rate of formaldehyde from flares is about 10-20 times greater than those found in the regulatory models developed by the Texas Commission on Environmental Quality's (TCEQ). This research will use air quality models to quantify the impact that increased formaldehyde emission from flares will have on Houston ozone concentrations. This study relies on the CAMx model (version 6.1) and emission data developed by Alpine Geophysics LLC (AG) and Climate & Atmospheric Research Associates (CARA) based on the combined databases from TCEQ, U.S. Environmental Protection Agency (EPA), and National Emission Inventory (NEI2008). This model also used meteorology data from the results of WRF-ARW dynamics. The CAMx generated process analysis data will also be used to quantify changes in radical budgets and NOx budgets critical to ozone production.

  7. 76 FR 43348 - Dana Structural Manufacturing, LLC, Structures Division, Longview, TX; Leased Workers From...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-20

    ...; Leased Workers From Manpower Working On-Site at Dana Structural Manufacturing, LLC Structures Division... Solutions Group, including on-site workers from Career Personnel, Longview, Texas, separated from employment... date being one full year before the petition date. There were no leased workers from Career...

  8. 78 FR 52716 - Proposed Amendment of Class E Airspace; Del Rio, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-26

    .... You may also submit comments through the Internet at http://www.regulations.gov . You may review the... comments a self-addressed, stamped postcard on which the following statement is made: ``Comments to Docket... through the Internet at http://www.regulations.gov . Recently published rulemaking documents can also...

  9. 78 FR 60237 - Proposed Amendment of Class E Airspace; Burnet, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-01

    ..., to request a copy of Advisory Circular No. 11-2A, Notice of Proposed Rulemaking Distribution System... airspace is necessary for the safety and management of IFR operations at the airport. Class E airspace... 15, 2013, which is incorporated by reference in 14 CFR 71.1. The Class E airspace designation...

  10. 78 FR 60235 - Proposed Amendment of Class E Airspace; Georgetown, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-01

    ...; #0; #0;#0;Federal Register / Vol. 78, No. 190 / Tuesday, October 1, 2013 / Proposed Rules#0;#0..., to request a copy of Advisory Circular No. 11-2A, Notice of Proposed Rulemaking Distribution System... airspace is necessary for the safety and management of IFR operations at the airport. Class E...

  11. Diurnal and seasonal variation of various carbon fluxes from an urban tower platform in Houston, TX

    NASA Astrophysics Data System (ADS)

    Schade, G. W.; Werner, N.; Hale, M. C.

    2013-12-01

    We measured carbon fluxes (CO2, CO, VOCs) from a tall lattice tower in Houston between 2007 and 2009, and 2011-2013. We present results from various analyses of (i) anthropogenic and biogenic CO2 fluxes using a quadrant segregation technique, (ii) seasonal and multi-year changes of CO fluxes as related to car traffic and industrial sources, and (iii) the accuracy of, and usefulness of a bulk flux footprint model to quantify pentane emissions form a distant source in comparison to permitted emission levels. Segregated and net anthropogenic CO2 fluxes were dominated by car traffic but industrial sources were identified as well. Emissions sank to minimal levels after hurricane Ike had passed over Houston, causing a traffic shutdown and lower population density. Segregated biogenic fluxes showed a clear seasonal variation with photosynthetic activity between April and November, and large effects of the 2011 Texas drought due to negligible irrigation in the study area. Carbon monoxide fluxes, measured via a flux gradient technique, are even stronger dominated by car traffic than CO2 fluxes and serve as a traffic tracer. Our data show a continued drop in emissions over time, seasonal changes with higher emissions during winter, and local influences due to industrial emissions. Lastly, we present the results of a tracer release study and a single point source quantification to test a bulk footprint model in this complex urban area. Known releases of volatile acetone and MEK were compered to measured fluxes using a REA-GC-FID system, and permit emissions of pentane from a foam plastics manufacturing facility were compared to measured pentane fluxes. Both comparisons reveal a surprisingly accurate performance of the footprint model within a factor of 2.

  12. 77 FR 32018 - Safety Zone; Kemah Boardwalk Summer Season Fireworks, Galveston Bay, Kemah, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-31

    ... significant effect on the human environment. This rule involves the establishment of a safety zone for the protection of human life. This rule is categorically excluded from further review under paragraph 34(g)...

  13. Space, our next frontier; Proceedings of the conference, Dallas, TX, June 7, 8, 1984

    SciTech Connect

    Musgrave, G.

    1985-01-01

    The present conference on space development encompasses space commercialization, legislative, legal, and insurance-related factors in current space programs, political aspects of space militarization and governmental control, the military future uses of space and their consequences, command and control issues arising in space, economic influences on space policy, and recent developments in space solar power generation concepts. Attention is given to public opinion surveys concerning the scientific, military, and economic uses of space, the Leasecraft orbital industrial infrastructure concept, capitalism and democracy in space development, the current status of space law on commercialization topics, the nature of Ballistic Missile Defense, the Soviet Space threat, the High Frontier concept for space defense, lunar solar power systems, solar power satellites, and the utilization of lunar resources for the reduction of lunar base construction costs. Such specific technical issues as microgravity crystal growth and directional solidification, electrophoresis operations for pharmaceuticals, and technical barriers to commercial access to space, are also noted.

  14. 77 FR 40406 - Cancellation of Supplemental Environmental Impact Statement (SEIS); Travis County, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-09

    ... Highway (SH) 71 West through Oak Hill, in Travis County, Texas, is being rescinded. FOR FURTHER... of US 290/SH 71 West through Oak Hill, in Travis County, Texas. The improvements proposed between... the NOI to prepare a SEIS for US 290/SH 71 West through Oak Hill from RM 1826 to Joe Tanner Lane....

  15. Texas Symposium on Relativistic Astrophysics, 11th, Austin, TX, December 12-17, 1982, Proceedings

    NASA Technical Reports Server (NTRS)

    Evans, D. S. (Editor)

    1984-01-01

    Various papers on relativistic astrophysics are presented. The general subjects addressed include: particle physics and astrophysics, general relativity, large-scale structure, big bang cosmology, new-generation telescopes, pulsars, supernovae, high-energy astrophysics, and active galaxies.

  16. Source apportionment of organic aerosol across Houston, TX during DISCOVER-AQ

    NASA Astrophysics Data System (ADS)

    Yoon, S.; Clark, A. E.; Ortiz, S. M.; Usenko, S.; Sheesley, R. J.

    2015-12-01

    As part of the ground-based sampling efforts during DISCOVER-AQ's Houston month-long campaign in September 2013, atmospheric particulate matter (PM) samples were collected at four sites: Moody Tower (urban), Manvel Croix (southern suburb), Conroe (northern suburb), and La Porte (urban industrial). The Houston metropolitan area, especially the Houston Ship Channel, is a densely industrialized urban city with large concentrations of petroleum refining, petrochemical manufacturing, and heavy traffic during peak hours. Due to these and other emission sources, the area is heavily impacted by ambient PM. This study will be looking at fine PM (diameter less than 2.5µm, PM2.5) from all four sites. PM2.5fraction is relevant for understanding fate and transport of organic contaminants and is widely known to negatively impact human health. Chemical analysis including radiocarbon (14C) and organic tracer measurements (polycyclic aromatic hydrocarbons, alkanes, hopanes, steranes, and levoglucosan) were used for source apportionment. The 14C measurements constrained CMB results to estimate both primary and secondary contributions to total organic carbon (TOC). Results indicate that Moody Tower had consistent primary motor vehicle exhaust contribution (18-27%) and a fossil secondary organic aerosol (SOA) contribution from 5-33% depending on atmospheric conditions. Conroe had a lower contribution of motor vehicle exhaust (5-10%) and similarly variable fraction of fossil SOA (4-25%). Manvel Croix had an interim motor vehicle contribution (9-15%) with a variable fossil SOA (5-30%). For contemporary OC, there was minimal contribution of wood smoke during examined weeks (0-9%) but larger contributor of biogenic SOA ranging from 40-75% at Moody Tower, 56-81% at Manvel Croix and 60-79% at Conroe. Overall, the motor vehicle contribution was consistent at each site during the analysis week, biogenic SOA was consistently high, while fossil SOA showed the most variability.

  17. 76 FR 13083 - Amendment to VOR Federal Airway V-358; TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-10

    ... coincides with the FAA's aeronautical database. DATES: Effective date 0901 UTC, May 5, 2011. The Director of... with the FAA's aeronautical database. Since this is an administrative change, and does not affect...

  18. Application of the TerreSIM Model to a Training Area Landscape at Fort Bliss, TX

    DTIC Science & Technology

    2005-09-01

    0.750 ERDC/CERL TR-05-26 53 Table B11. Physiological response months. Species Green-out Seed - Sprout Seed - Set Dormancy Broom snakeweed...Physiological control constraints. Species Growing season max root:shoot Growing season green-out shoot:root Max 1-mo seed germination Max...maximum root:shoot ratio. Maximum 1-month seed germination is the proportion of the seed bank for a par- ticular species that can germinate in any

  19. 77 FR 47660 - Attwater Prairie Chicken National Wildlife Refuge, Austin and Colorado Counties, TX; Final...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-09

    ... refuge. Wildlife Management Issue 3: Manage three food plots Same as Alternative A; Eliminate wildlife food Wildlife Food Plots (Farming totaling up to 150 acres. plus explore additional plots. Program... addition of food plots when the species' populations expand. Visitor Services Issue 1: Provide...

  20. 76 FR 77245 - Attwater Prairie Chicken National Wildlife Refuge, Austin and Colorado Counties, TX...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-12

    ... 3: Manage three food Same as Alternative A; plus Eliminate wildlife Wildlife Food Plots (Farming plots totaling up to explore additional ways to food plots. Program). 150 acres. provide supplemental food to Attwater's prairie- chicken, including capability to irrigate and addition of food plots...

  1. 77 FR 40901 - Notice of Inventory Completion: Gregg County Historical Museum, Longview, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-11

    ... one possibly male adult, one adult of unknown sex, and one juvenile of unknown sex. The human remains from Burial 6 include an occipital cranial bone fragment of one adult of unknown sex. The human remains from Burial 7 include one adult of unknown sex. No known individuals were identified. The 11...

  2. 33 CFR 165.808 - Corpus Christi Ship Channel, Corpus Christi, TX, safety zone.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... departures of loaded LPG vessels via a Marine Safety Information Broadcast Notice to Mariners. ... established as safety zones during specified conditions: (1) For incoming tank vessels loaded with Liquefied... Christi Ship Channel to the LPG receiving facility. The safety zone remains in effect until the LPG...

  3. 33 CFR 165.808 - Corpus Christi Ship Channel, Corpus Christi, TX, safety zone.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... departures of loaded LPG vessels via a Marine Safety Information Broadcast Notice to Mariners. ... established as safety zones during specified conditions: (1) For incoming tank vessels loaded with Liquefied... Christi Ship Channel to the LPG receiving facility. The safety zone remains in effect until the LPG...

  4. 33 CFR 165.808 - Corpus Christi Ship Channel, Corpus Christi, TX, safety zone.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... departures of loaded LPG vessels via a Marine Safety Information Broadcast Notice to Mariners. ... established as safety zones during specified conditions: (1) For incoming tank vessels loaded with Liquefied... Christi Ship Channel to the LPG receiving facility. The safety zone remains in effect until the LPG...

  5. 75 FR 27493 - Proposed Amendment of Class E Airspace; Center, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-17

    ... with the FAA's National Aerospace Charting Office. Controlled airspace is needed for the safety and... 6.5-mile radius of Center Municipal Airport and within 2.5 miles each side of the 341 bearing from....3 miles each side of the 171 bearing from the airport extending from the 6.5-mile radius to...

  6. Freshwater Sustainability under Climate Change in the Nueces River Basin, TX

    NASA Astrophysics Data System (ADS)

    Gonzalez, D. A.; Sinha, T.; Ji, J. H.

    2015-12-01

    Freshwater sustainability in arid and semi-arid regions is threatened due to climate change as well as competing water demands for agriculture, urban development, industrial use and ecosystem needs. Such changes have forced the local water supply systems to update their water supply plans once in five years. Developments of such water supply plans not only require reliable assessment of water availability and demands but also incorporate uncertainties due to regional climate change projections. In this study, we focus our analysis on one of the basins in the South Texas - Nueces River Basin (NRB) which provide inflows to the Choke Canyon - Lake Corpus Christi reservoir system. This reservoir system is the major freshwater source for the coastal communities in the basin and the Nueces Bay, which serve as a habitat for several key fish species such as blue crab, brown shrimp, and southern flounder. Freshwater inflows in the NRB have decreased in the past decades, resulting in increased salinity of the Nueces Bay, thus impacting the natural habitat for several fish species. Therefore, estimating the impacts of climate change in the NRB is critical to develop sustainable water resources management in the region. We will implement a physically based hydrologic model under historical climate change scenarios from multiple General Circulation Models (GCMs) over the past 30 years to understand how well we could have used large scale climate change projections in improving water resources management over the overlapping observations. A water management model will be developed for the Choke Canyon - Lake Corpus Christi Reservoir System, which will be ingested with inflow projections under multiple GCM scenarios over the past 30 years to incorporate uncertainty in water resources management. Finally, water management scenarios will be developed to minimize deficits between water availability and demands in the region.

  7. 75 FR 9635 - Cancellation of Environmental Impact Statement; Harris and Brazoria Counties, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-03

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF TRANSPORTATION Federal Highway Administration Cancellation of Environmental Impact Statement; Harris and Brazoria... between Bellfort Road and FM 1462 in Harris and Brazoria Counties, Texas. The use of Federal funding...

  8. Headaches in older patients: Ddx and Tx of common nonvascular causes.

    PubMed

    Warner, J J

    1985-11-01

    From this and the previous article, the following points may be offered in summary: When comparing the elderly age group with the general population, the incidence of migraine headaches decreases with age, whereas other etiologies such as glaucoma, temporal arteritis, and cerebrovascular disease may assume a more prominent role in the differential diagnosis. Patients in the geriatric population are frequently taking a multitude of medications, and it is extremely important to carefully evaluate these for possible precipitants of headache. Furthermore, in elderly patients with other potential medical problems, particular attention should be paid to the possibility of various systemic causes of headache. Therapy for specific headache disorders should be tailored to the individual patient. Consider the patient's overall general, psychological, medical, and neurologic background. The physician must be aware of possible interactions of medications with the therapeutic intervention, as well as possible poor tolerance to specific medications due to preexisting medical or neurologic disorders. A complete history, obtaining information on the temporal pattern of headache, the distribution of pain, and precipitating and alleviating factors, is extremely important in evaluating the elderly patient. A careful physical examination, paying particular attention to possible disorders of extracranial structures, is indicated. A neurologic exam, including basic tests of higher cortical function, should be obtained. Important additional laboratory investigations include a complete blood count, erythrocyte sedimentation rate, and basic blood chemistries. Arterial blood gases should be obtained in patients who have pulmonary disease, a history suggestive of sleep apnea, or other disorders that may produce hypoxia and hypercarbia, resulting in vascular headache.(ABSTRACT TRUNCATED AT 250 WORDS)

  9. MIMO Radar System for Respiratory Monitoring Using Tx and Rx Modulation with M-Sequence Codes

    NASA Astrophysics Data System (ADS)

    Miwa, Takashi; Ogiwara, Shun; Yamakoshi, Yoshiki

    The importance of respiratory monitoring systems during sleep have increased due to early diagnosis of sleep apnea syndrome (SAS) in the home. This paper presents a simple respiratory monitoring system suitable for home use having 3D ranging of targets. The range resolution and azimuth resolution are obtained by a stepped frequency transmitting signal and MIMO arrays with preferred pair M-sequence codes doubly modulating in transmission and reception, respectively. Due to the use of these codes, Gold sequence codes corresponding to all the antenna combinations are equivalently modulated in receiver. The signal to interchannel interference ratio of the reconstructed image is evaluated by numerical simulations. The results of experiments on a developed prototype 3D-MIMO radar system show that this system can extract only the motion of respiration of a human subject 2m apart from a metallic rotatable reflector. Moreover, it is found that this system can successfully measure the respiration information of sleeping human subjects for 96.6 percent of the whole measurement time except for instances of large posture change.

  10. DOE Zero Energy Ready Home Case Study: M Street Homes — Smartlux on Greenpark, Houston, TX

    SciTech Connect

    none,

    2014-09-01

    This builder certified its first DOE Zero Energy Ready Home and won a Production Builder honor in the 2014 Housing Innovation Awards. It is the first home in the world to use a tri-generation system to supply electricity, heating, and cooling on site.

  11. Completion of One Year Bioventing Test, Jet Fuel Storage Tank #20, Randolph AFB, TX

    DTIC Science & Technology

    2007-11-02

    the contractor from collecting the soil samples after the bioventing system had been operating one year. Soil gas sampling results showed a three order of magnitude reduction in TVH at MPA-6 and MPC-6.

  12. Lunar and Planetary Science Conference, 21st, Houston, TX, Mar. 12-16, 1990, Proceedings

    SciTech Connect

    Ryder, G.; Sharpton, V.L.

    1991-01-01

    The present conference on lunar and planetary science discusses the geology and geophysics of Venus; the lunar highlands and regolith; magmatic processes of the moon and meteorites; remote sensing of the moon and Mars; chondrites, cosmic dust, and comets; ammonia-water mixtures; and the evolution of volcanism, tectonics, and volatiles on Mars. Attention is given to volcanism on Venus, pristine moon rocks, the search for Crisium Basin ejecta, Apollo 14 glasses, lunar anorthosites, the sources of mineral fragments in impact melts 15445 and 15455, and argon adsorption in the lunar atmosphere. Also discussed are high-pressure experiments on magnesian eucrite compositions, the early results of thermal diffusion in metal-sulfide liquids, preliminary results of imaging spectroscopy of the Humorum Basin region of the moon, high-resolution UV-visible spectroscopy of lunar red spots, and a radar-echo model for Mars. Other topics addressed include nitrogen isotopic signatures in the Acapulco Meteorite, tridymite and maghemite formation in an Fe-SiO smoke, and the enigma of mottled terrain on Mars.

  13. 76 FR 4818 - Drawbridge Operation Regulation; Old Brazos River, Freeport, Brazoria County, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-27

    ... operation of the Union Pacific Railroad Swing Span Bridge across the Old Brazos River, mile 4.4, at Freeport... February 10, 2011 until 7 a.m. on February 16, 2011. This deviation will allow the swing span of the bridge to remain in the closed-to-navigation position in order for the north end of the swing span to be...

  14. 78 FR 45909 - Designation for the Amarillo, TX; Cairo, IL; Baton Rouge, LA; Raleigh, NC; and Belmond, IA Areas

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-30

    ..., Inc. (Schaal) to provide official services under the United States Grain Standards Act (USGSA), as... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE..., Inc. (Amarillo), Cairo Grain Inspection Agency, Inc. (Cairo), Louisiana Department of Agriculture...

  15. 77 FR 6780 - Designation for the State of Alabama; Saginaw, TX; Essex, IL; Springfield, IL; Savage, MN; and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-09

    ... Agriculture (Washington) to provide official services under the United States Grain Standards Act (USGSA), as... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Grain Inspection, Packers and Stockyards Administration Designation for the State of Alabama;...

  16. Test Review: D. Wechsler "Wechsler Adult Intelligence Scale" (4th ed.). San Antonio, TX--Psychological Corporation, 2008

    ERIC Educational Resources Information Center

    Climie, Emma A.; Rostad, Kristin

    2011-01-01

    This article presents a review of the "Wechsler Adult Intelligence Scale-Fourth Edition" (WAIS-IV), an individually administered measure of cognitive ability for individuals aged 16 years, 0 months to 90 years, 11 months. The WAIS-IV was designed with a number of specific goals including updated norms, increased user friendliness,…

  17. 2013 plant lipids Gordon Research conference and Gordon Research Seminar (January 27 - February 1, 2013 - Hotel Galvez, Galveston, TX)

    SciTech Connect

    Welti, Ruth

    2012-11-01

    Presenters will discuss the latest advances in plant and algal lipid metabolism, oil synthesis, lipid signaling, lipid visualization, lipid biotechnology and its applications, the physiological and developmental roles of lipids, and plant lipids in health. Sessions include: Producing Nutritional Lipids; Metabolic biochemistry in the next decade; Triacylglycerols: Metabolism, function, and as a target for engineering; Lipids in Protection, Reproduction, and Development; Genetic and Lipidomic Approaches to Understanding Lipid Metabolism and Signaling; Lipid Signaling in Stress Responses; New Insights on the Path to Triacylglycerols; Membrane Lipid Signaling; Lipid Visualization; Development of Biofuels and Industrial Lipids.

  18. Test Review: Wechsler, D. (2005). "Wechsler Intelligence Scale for Children-Fourth Edition Spanish." San Antonio, TX: Harcourt Assessment

    ERIC Educational Resources Information Center

    Braden, Jeffery P.; Iribarren, Jacqueline A.

    2007-01-01

    In this article, the authors review the Wechsler Intelligence Scale for Children-Fourth Edition Spanish (WISC-IV Spanish), a Spanish translation and adaptation of the WISC-IV. The test was developed to measure the intellectual ability of Spanish-speaking children in the United States ages 6 years, 0 months, through 16 years, 11 months. These…

  19. Lunar and Planetary Science Conference, 15th, Houston, TX, March 12-16, 1984, Proceedings. Part 2

    NASA Technical Reports Server (NTRS)

    Ryder, G. (Editor); Schubert, G. (Editor)

    1985-01-01

    Subjects of lunar petrology are discussed, taking into account Apollo 14 aluminous mare basalts and their possible relationship to KREEP, the petrology and geochemistry of clasts from consortium breccia, the depths of the mare basalt source region, the origin of olivine at Copernicus, a transient heating event in the history of a highlands troctolite from Apollo 12 soil, and the composition and evolution of the lunar crust in the Descartes highlands. Other topics explored are related to early earth and magmatic processes, differentiated meteorites, chondritic meteorites, other planets and remote sensing, and cratering. Attention is given to the gravity field of Venus at constant altitude and comparison with earth, a spectral analog of Martian soil, dark halo craters and the thickness of grooved terrain on Ganymede, the geomorphology of Rhea, a Monte Carlo model of lunar megaregolith development, the scaling of complex craters, crustal radiogenic heat production and the selective survival of ancient continental crust, and the formation of an impact-generated H2O atmosphere and its implications for the early thermal history of the earth.

  20. Test Review: Wechsler, D. (2005). "Wechsler Intelligence Scale for Children--Four Edition Spanish." San Antonio, TX: Psychological Corporation

    ERIC Educational Resources Information Center

    Clinton, Amanda

    2007-01-01

    In this article, the author reviews the Wechsler Intelligence Scale for Children--Four Edition Spanish (WISC-IV Spanish), an individually administered measure of intelligence for Spanish-speaking children who are English language learners and relatively new to American culture. The WISC-IV Spanish, like its English counterpart, the WISC-IV, is…