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Sample records for age-0 channel catfish

  1. Channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This book chapter provides a comprehensive overview of channel catfish aquaculture. Sections include fish biology; commercial culture; culture facilities; production practices; water quality management; nutrition, feeding and feed formulation; infectious diseases; harvesting and processing; and the...

  2. Channel catfish pond fertilization

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The most successful aquaculture enterprise in the U.S. is channel catfish Ictalurus punctatus production. In the U.S., 257 million kg of channel catfish were sold in 2007 at a value of $455 million. Large-scale commercial channel catfish culture began in the late 1950s and expanded rapidly from 1978...

  3. Production and Processing Traits of Blue Catfish, Blue Catfish X Channel Catfish Hybrids, and Two Strains of Channel Catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish is the primary species used in commercial catfish farming. However, increased incidence of disease and associated economic losses have led to speculation that other catfish species or hybrids also have potential. In this study production and processing traits of blue catfish, blue x ...

  4. Flavobacterium columnare chemotaxis to channel catfish mucus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Flavobacterium columnare is a Gram-negative pathogen of many species of wild and cultured fish, especially channel catfish (Ictalurus punctatus). This motile microbe is responsible for severe economic losses to the catfish industry. Flavobacterium columnare isolates from diseased channel catfish b...

  5. Evaluation of immune responses against the parasite Ichthyophthirius multifiliis between channel catfish and hybrid catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish is a dominant aquaculture species in the USA and has been cultured for several decades. Recently, an increasing number of producers are showing an interest in the culture of hybrid catfish (channel catfish × blue catfish) instead of channel catfish due to its performance traits. The ...

  6. Pyridoxine requirements of channel catfish.

    PubMed

    Andrews, J W; Murai, T

    1979-04-01

    In 20 and 12 week feeding trials, channel catfish fingerlings were fed purified diets containing five levels (0, 5, 10, 20, and 30 mg/kg) and six levels (0, 1, 2, 5, 10, and 20 mg/kg) of supplemental pyridoxine hydrochloride. Fish fed unsupplemented diets (pyridoxine content of 1.2 mg/kg) were characterized by anoxeria, nervous disorders, tetany, greenish-blue body coloration, and eventual mortality. Anemia, which has been reported in pyridoxine deficient salmonids, was not observed in pyridoxine deficient catfish. However, a microcytic, normochromic anemia was observed in groups fed high dietary levels of pyridoxine (20 mg/kg or greater). The dietary pyridoxine level required for maximal growth was approximately 3 mg/kg of diet. All other deficiency signs were prevented by 2.2 mg/kg of diet. PMID:430257

  7. Differential Susceptibility of Blue Catfish, Channel Catfish, and Blue X Channel Catfish Hybrids to Channel Catfish Virus (CCV)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    CCV is the major viral pathogen of channel catfish. Although it accounts for only approximately 5% of reported cases of the mortality in fry and fingerlings, viral outbreaks can spread rapidly and have disastrous results. Previous reports from the literature suggest that when natural routes of infec...

  8. The LOX calculator for fasted channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish (Ictalurus punctatus) are typically harvested by seining, and are then transferred to a grading net, where the catch is held overnight. Respiratory demands of a large, confined channel catfish biomass can exceed available dissolved oxygen, so supplemental aeration is necessary. In 20...

  9. Genetic Response To Selection In Channel Catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish selected solely for fast growth (fast growing) were compared to USDA103, USDA403, and industry pool groups of channel catfish for growth, body composition, and stress response. All fish were fed daily for 8 wks followed by an acute 10-minute dewatering stress. By wk 4, feed intake ...

  10. Pathogenicity of Streptococcus ictaluri to Channel Catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The infectivity of a Streptococcus ictaluri isolate for fry (0.5 g), fingerling (15 g), and juvenile (55 g) channel catfish (Ictalurus punctatus) was determined by bath immersion and injection infectivity experiments. Channel catfish exposed by immersion were exposed to baths containing 1012, 1011,...

  11. The effect of calcium hardness on hatching success of channel catfish x blue catfish hybrid catfish eggs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The present study was designed to determine the optimal level of calcium hardness in hatching waters to incubate channel catfish Ictalurus punctatus ' x blue catfish I. furcatus ' hybrid catfish eggs. Hatching success of hybrid catfish eggs was higher (p<0.05) at 75 mg L-1 of calcium hardness (C...

  12. Production characteristics of channel catfish and channel x blue catfish in earthen ponds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The channel (female) x blue (male) hybrid catfish increasingly is being selected by catfish farmers in the United States as their culture fish. This completely randomized design study in 2 x 2 factorial arrangement was conducted to compare production traits in earthen ponds of the channel x blue hyb...

  13. Hormone induced spawning of channel catfish Ictalurus punctatus in channel catfish x blue catfish f1 hybrid catfish embryo production cpe vs lhrha

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Superior performance of channel x blue hybrid catfish in production ponds coupled with consistent availability of fingerlings to food fish farmers have made hybrid catfish a most desired aquaculture species for production in the catfish industry. Projected hybrid fingerling production in 2011 is...

  14. C DNA SEQUENCE OF CHANNEL CATFISH PEROXIREDOXIN 6 GENE

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Peroxiredoxin 6 gene (Prdx6) of channel catfish, Ictalurus punctatus, was cloned and sequenced. Total RNA from channel catfish tissues was isolated, reverse transcribed and amplified. The sequence of the channel catfish Prdx6 gene consists of 1003 nucleotides. Analysis of the nucleotide sequence ...

  15. Effects of Low Oxygen on Production of Blue Catfish Ictalurus furcatus and Channel Catfish I. punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Blue catfish (Ictalurus furcatus) have several advantages over channel catfish (I. punctatus) as commercial culture fish. They are more resistant to ESC, PGD, and CCVD, major diseases of channel catfish. They are very easy to seine, with a near-total harvest possible with one seine haul, and may ex...

  16. Comparative Oxygen Tolerance of Blue Catfish Ictalurus furcatus and Channel Catfish I. punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Blue catfish (Ictalurus furcatus) have several advantages over channel catfish (I. punctatus) as commercial culture fish. They are more resistant to ESC, PGD, and CCVD, major diseases of channel catfish. They are very easy to seine, with a near-total harvest possible with one seine haul, and may ex...

  17. Comparative growth and yield of channel catfish and channel x blue hybrid catfish fed a full or restricted ration

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Growth and yield (kg ha-1) of the channel catfish (Ictalurus punctatus, Rafinesque, 1818) and the channel x blue hybrid catfish (I. punctatus female x I. furcatus [Lesueur, 1840] male), which shared the Jubilee strain of channel catfish as the maternal parent, were compared in 16 0.1-ha earthen pond...

  18. Comparative production of channel catfish and channel x blue hybrid catfish subjected to two minimum dissolved oxygen concentrations

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The effect of daily minimum dissolved oxygen concentration on growth and yield (kg/ha) of the channel catfish (Ictalurus punctatus) and the channel x blue hybrid catfish (I. punctatus female x I. furcatus male), which shared the Jubilee strain of channel catfish as the maternal parent, was evaluated...

  19. Hybrid, channel catfish show similar immune responses to Ich parasite

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The hybrid catfish (female channel catfish × male blue catfish) has been reported to have some commercially desirable characteristics, such as faster growth, better feed conversion, low oxygen tolerance, higher fillet yields, and resistance to some diseases. Previous studies showed that channel catf...

  20. Cart Regulates Food Intake in Channel Catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cocaine-and Amphetamine-Regulated Transcript (CART) is a potent hypothalamic anorectic peptide in mammals and fish. We hypothesized that increased food intake is associated with changes in expression of CART mRNA within the brain of channel catfish. Objectives were to clone the CART gene, examine ...

  1. Recent improvements in channel catfish reproduction

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Control of reproduction in fish is a primary requisite for reliable, predictable and quality seed stock for aquaculture production or a stock enhancement program. Channel catfish is the leading aquaculture species in USA, accounting for 335 million pounds with a farm gate value of over $ 400 millio...

  2. DEET toxicity to channel catfish sac fry

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The combination of open facilities, moisture, and warm weather during channel catfish Ictalurus punctatus spawning season causes mosquito-infestation problems. A common solution to mosquito problems in hatcheries is to use mosquito repellents applied to exposed skin and clothing. DEET (N,N-diethyl-...

  3. Plankton Management for Channel Catfish Nursery Ponds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We conducted a series of studies examining the fertilization practices used for channel catfish nursery ponds. The best fertilization protocol would be one that uses low-cost fertilizers, quickly establishes a desirable phytoplankton bloom, and produces the greatest number of large zooplankton. In...

  4. Bacterial Bioaugmentation of Channel Catfish Ponds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Twelve, 0.1-ha earthen ponds at Stoneville, Mississippi were used in a 2-year, double-blind study of the effects of a Bacillus-based bacterial bioaugmentation product on water quality and production of channel catfish Ictalurus punctatus. Each year, six ponds were treated weekly with the microbial p...

  5. The influence of ovarian fluid ph of stripped unfertilized channel catfish Ictalurus punctatus eggs on the hatching success of channel catfish x blue catfish I. furcatus hybrid catfish eggs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study was designed to determine the effect of ovarian fluid pH of stripped unfertilized channel catfish (Ictalurus punctatus) eggs on fertilization and hatch rate of channel catfish ' x blue catfish (I. furcatus) ' hybrid catfish eggs. A significant correlation was established between ovarian...

  6. Calcium deprivation during channel catfish, Ictalurus punctatus X blue catfish, I. furcatus F1 hybrid catfish embryo development affects hatching success

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel x blue hybrid catfish are produced by fertilizing eggs from hormone-induced, strippable channel catfish (Ictalurus punctatus) females with sperm from blue catfish (I. furcatus). Many catfish hatchery supplies have low calcium concentrations and are supplemented with an external source of ca...

  7. Growth of channel catfish and channel x blue hybrid catfish in ponds at restricted or satiate feeding rate

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Growth and yield of channel catfish Ictalurus punctatus and channel x blue hybrid catfish I. punctatus x I. furcatus were evaluated in a completely randomized design study in 2 x 2 factorial arrangement that was conducted in 16 0.25-ac earthen ponds. Factor 1 was strain (channel or channel x blue hy...

  8. Nutritional Regulation of IGFs in Channel Catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We examined changes in hepatic IGF-I and IGF-II mRNA, insulin like growth factor binding proteins (IGFBP-1 and IGFBP-2) mRNA, muscle IGF-I and IGF-II mRNA in fed (fed daily for 45 days) and restricted (not fed for 30 days followed by feeding for 15 days) channel catfish. By day 30, liver IGF-I mRNA...

  9. Effects of nutrient enrichment on channel catfish growth and consumption in Mount Storm Lake, West Virginia

    USGS Publications Warehouse

    Blanc, T.J.; Margraf, F.J.

    2002-01-01

    With the objective of augmenting fish production in Mount Storm Lake, Virginia Electric and Power Company initiated a programme of phosphorus addition to increase primary production, and ultimately, channel catfish (Ictaturus punctatus) growth in the 486 ha cooling reservoir. We simulated channel catfish growth dynamics using two bioenergetics modelling scenarios: (i) effects of average reservoir temperature on growth, conversion efficiency and consumption; and (ii) effects of reservoir enrichment on growth, which is simulated by increasing feeding rates. During 1991-1993, fish were sampled monthly, but sampling was increased to every 2 weeks during the peak growing season (June-September). Most of the channel catfish collected were aged 0 year and aged 1 year with rapid annual growth rates ranging from 9.0 to 13.7 J/g. We found many age 1 250-300 mm catfish, but few beyond this size. Conversion efficiency (joules gained/joules consumed) was low at approximately 18-19%. High algae consumption (40%) was evident, but consumption of zooplankton and Asiatic clam (Corbicula sp.) increased over the study. Simulated increased feeding rates showed that channel catfish were food limited in summer and fall (July-December). Weight gains with 5 and 10% feeding increases were 6-13% and 18-38%, respectively, from the baseline. Catfish of all sizes should benefit from phosphorus additions.

  10. Potency of blue catfish, Ictalurus furcatus (individual vs pooled) sperm to fertilize stripped channel catfish, I. punctatus eggs on the production and performance of progeny

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel x blue hybrid catfish is the desired genotype for US farm-raised catfish industry. Induced spawning of gravid channel catfish, followed by fertilization of stripped eggs with blue catfish sperm is the only reliable means to produce hybrid catfish embryos in hatcheries. Hybrid catfish fry p...

  11. CONCURRENT EXPERIMENTAL Streptococcus SPP. INFECTIONS AND NATURAL PARASITISM IN CHANNEL CATFISH Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Streptococcus iniae and S. agalactiae are usually not considered pathogens of channel catfish, Ictalurus punctatus, though concurrent infections may decrease catfish survival when infected with streptococcal organisms. Non-parasitized or naturally-parasitized channel catfish fry were challenged wit...

  12. Low-Intensity channel catfish culture re-visited

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish (Ictalurus punctatus) farmers can use a variety of management strategies to produce the larger-sized fish that processing plants increasingly seek. A low-intensity production strategy based on low stocking and aeration rates can be used to produce larger channel catfish. Stocker catf...

  13. Low-intensity, stocker-based channel catfish culture

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Low-intensity Channel Catfish production is characterized by low stocking rates, low installed aeration capacity, and no automated dissolved oxygen monitoring. Two studies conducted in nine 0.25-acre ponds quantified production characteristics of stocker Channel Catfish stocked for low-intensity foo...

  14. Pretreating channel catfish with copper sulfate affects susceptibility to columnaris

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Columnaris disease is a costly bacterial disease of commercially grown channel catfish. Three experiments were conducted to evaluate the effects of pre-exposing channel catfish fingerlings to 0, 1/2, 1, or 2 times the recommended copper sulfate rate for 24 hours on their susceptibility to columnaris...

  15. The safety of copper sulfate to channel catfish eggs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper sulfate (CuSO4) is an economical treatment to control fungus (Saprolegnia spp.) on channel catfish eggs and is widely used by the industry. The purpose of this study was to determine the safety of copper sulfate to channel catfish eggs when treated at the therapeutic rate (10 mg/L), and also...

  16. A liquid oxygen calculator for fasted channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An interactive liquid oxygen (LOX) calculator for fasted channel catfish confined in grading nets or in live haul tanks has been developed, using Microsoft Visual Studio 2005©. The calculator is based on results of scientific experiments on channel catfish metabolism, and estimates oxygen consumptio...

  17. Effects of DO cencentration on growth of juvenile channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Dissolved oxygen (DO) concentration has a major impact on feed consumption of channel catfish when raised in ponds; as DO concentration falls below 3.0 mg/L at night, feed consumption is negatively impacted. Channel catfish fry may experience a wide range of oxygen conditions in the hatchery depend...

  18. A liquid oxygen calculator for fasted channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A review of scientific literature concerning channel catfish Ictalurus punctatus respiration resulted in development of a Microsoft Excel© spreadsheet for estimating the volume of oxygen consumed by a given fasted channel catfish biomass. Entry of ten variables into the spreadsheet provides estimate...

  19. Production of channel catfish and channel x blue hybrid catfish subjected to two minimum dissolved oxygen concentrations

    Technology Transfer Automated Retrieval System (TEKTRAN)

    As the channel x blue hybrid catfish is stocked by an increasing number of catfish farmers, it is important to quantify the production response of this fish to dissolved oxygen management strategies. The purpose of this study was to compare the production and water quality responses of the channel x...

  20. Effects of DO concentration in growout performance of blue catfish with comparison to channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Feed intake, feed conversion ratio (FCR), and production of blue catfish Ictalurus furcatus and channel catfish I. punctatus were examined in one-acre ponds maintained at either a high or low (means of 4.29 and 2.54 ppm, [54.8 and 32.4 % air saturation] respectively) minimum dissolved oxygen (DO) co...

  1. Method for inducing saprolegniasis in channel catfish

    USGS Publications Warehouse

    Howe, G.E.; Rach, J.J.; Olson, J.J.

    1998-01-01

    A method was developed to uniformly and systematically induce saprolegniasis in channel catfish Ictalurus punctatus. Three different methods for inducing saprolegniasis were evaluated in waters containing known zoospore concentrations of Saprolegnia parasitica (1) low-temperature shock to induce immunosuppression: (2) physical abrasion stress; and (3) a combination of both low temperature shock and abrasion stress. Low-temperature shock or abrasion stress alone were not effective for inducing saprolegniasis. Only 10% of fish stressed by low-temperature shock alone became infected. No fish receiving abrasion stress treatments alone became infected even though these fish were subject to significant abrasion and dewatering stress. A combination of low-temperature and abrasion stress, however, was sufficient to induce saprolegniasis in 100% of fish tested and resulted in 90% mortality. No fish became infected in the positive control group (exposed to zoospores of S. Parasitica without stress) or in the negative control group. The combined-stress method should enable researchers to induce saprolegniasis in channel catfish at will to study its pathogenesis or to test the efficacy of candidate antifungal treatments. In conducting efficacy studies, therapeutic treatments must begin immediately when the first signs of saprolegniasis are observed because the disease progresses quickly and is deadly.

  2. PROTECTIVE EFFECT OF CUTANEOUS ANTIBODY AGAINST ICHTHYOPHTHIRIUS ON CHANNEL CATFISH

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fish recovered from sublethal ichthyophthiriasis acquire protective immunity against Ichthyophthirius (Ich). The objective of this study was to evaluate the protective effect of cutaneous antibody produced by channel catfish immune to Ich on cohabited non-immune catfish after exposure to 15,000 ther...

  3. Routine Metabolic Rate of Channel Catfish Ictalurus punctatus Fry

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish eggs are typically incubated at high density and are often subjected to sub-optimum dissolved oxygen (DO) concentrations while in the hatchery. Since DO plays an important role in the development, hatch rate, and growth of catfish eggs and fry, we measured routine metabolic rate of ...

  4. Essential oils increase weight gain in channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We examined the effects of matrix encapsulated essential oils (Biomin® P.E.P. MGE) on weight gain, specific growth rate (SGR), feed conversion ratio (FCR), and survival of channel catfish. Five hundred catfish (32.4 ± 1.7 g/fish) were randomly assigned to two treatments with five replicate tanks/tre...

  5. Essential oils enhance fillet composition of channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We investigated the effects of a phytogenic feed additive (Digestarom® P.E.P. MGE) on growth performance, processing yield, fillet composition, and survival of pond raised channel catfish. Fifteen 0.4 ha ponds were stocked with 14,820 catfish (126 g/fish) per ha. Fish in control ponds were fed a 3...

  6. Safety of copper sulfate to channel catfish eggs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper sulfate (CuSO4) is commonly used in the catfish industry to control saprolegniasis (caused by watermolds) on eggs. This study was designed to establish the safety of CuSO4 when applied to hatching troughs containing channel catfish eggs in 26 degrees C flow-through well water at 10, 30, and ...

  7. Spermatogonial stem cells specific marker identification in channel catfish, Ictalurus punctatus and blue catfish, I. furcatus.

    PubMed

    Shang, Mei; Su, Baofeng; Lipke, Elizabeth A; Perera, Dayan A; Li, Chao; Qin, Zhenkui; Li, Yun; Dunn, David A; Cek, Sehriban; Peatman, Eric; Dunham, Rex A

    2015-12-01

    Testicular germ cells of channel catfish, Ictalurus punctatus, and blue catfish, I. furcatus were separated into four layers with Percoll density gradient centrifugation, containing different cell types (40% in the first layer were spermatogonial stem cells, SSCs). Expression of seventeen genes was analyzed for cells from different layers by real-time quantitative PCR. Pfkfb4, Urod, Plzf, Integrin6, IntegrinV, Thy1 and Cdh1 genes showed the same expression change pattern in both channel and blue catfish as these genes were down-regulated in the spermatocytes and even more so in spermatids. Plzf and Integrin6 had especially high expression in SSCs and can be used as SSCs specific markers. Sox2 gene was up-regulated in spermatocytes and even more highly up-regulated in spermatids, which indicated it could be a spermatid marker. In contrast to channel catfish, Id4, Smad5 and Prdm14 gene expressions were strongly down-regulated in spermatocyte cells, but up-regulated in spermatid cells in blue catfish. Smad5 gene was down-regulated in spermatocytes, but up-regulated in both spermatogonia and spermatids, allowing identification as a marker for spermatocytes in blue catfish. Oct4, Id4, Gfrα2, Pum2 and Prdm14 genes showed different expression patterns in the testicular germ cells of channel and blue catfish. This may be a partial explanation to the differing responses of channel catfish and blue catfish to induced spawning technologies. The SSCs specific markers can be used for further SSCs labeling, which can increase the SSCs sorting efficiency and be applied in various studies involving SSCs and other germ cells. PMID:26251285

  8. Water hardness influences Flavobacterium columnare pathogenesis in channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Studies were conducted to determine aspects of water chemistry responsible for large differences in pathogenesis and mortality rates in challenges of channel catfish Ictalurus punctatus with Flavobacterium columnare; challenges were conducted in water supplying the Stuttgart National Aquaculture Res...

  9. Probiotic Feeding Experiments with Juvenile Channel Catfish and Nile Tilapia

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Probiotic microbial feed supplements are gaining wide acceptance in livestock production, and may be applicable to aquaculture production systems. We have conducted a series of experiments using commercial probiotics added to diets of juvenile channel catfish, Ictalurus punctatus (Rafinesque), and...

  10. Influence of dihydrotestosterone on sex determination in channel catfish and blue catfish: period of developmental sensitivity.

    PubMed

    Davis, K B; Goudie, C A; Simco, B A; Tiersch, T R; Carmichael, G J

    1992-04-01

    Treatment of channel catfish with 0.2, 20, or 200 mg/liter of dihydrotestosterone (DHT) in the water during the egg stage or during egg and sac-fry stages did not alter the expected 1:1 sex ratio of the progeny. Feeding DHT at 200 mg/kg of feed for the first 21 days after yolk sac absorption resulted in 80% females; this proportion was increased by combining feeding with treatment of 200 mg DHT/liter in the sac-fry stage (90%) or in the egg and sac-fry stage (97%). In contrast, treatment of blue catfish sac-fry with 200 mg DHT/liter, with or without the combination of feeding DHT at 200 mg/kg food, resulted in 100% female populations. Neither clomiphene citrate, an estrogen-receptor blocking agent, nor clofibrate, an inhibitor of hepatic synthesis of cholesterol, affected the sex ratio of channel catfish, and neither of these compounds altered the feminizing effect of 200 mg DHT/kg when fed in combination with DHT. The nonaromatizable androgen DHT is not as effective as many other androgens in producing paradoxical female populations of channel catfish. However, feminization of blue catfish by treatment of sac-fry indicates that this species is more susceptible to hormonal manipulation and that the period of sex determination may occur earlier in development than in channel catfish. PMID:1505724

  11. Efficacy of salmon GnRHa to stimulate ovulation in channel catfish Ictalurus punctatus in hybrid catfish fry production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Hormone manipulations for the induction of maturation and ovulation have made possible the control of reproductive processes in channel catfish and have contributed significantly to the production of hybrid catfish in the catfish industry. Hybrid embryo production requires more labor, resources, an...

  12. Nitrite-induced anemia in channel catfish, Ictalurus punctatus Rafinesque

    SciTech Connect

    Tucker, C.S. ); Francis-Floyd, R.; Beleau, M.H. )

    1989-08-01

    Since 1983 numerous cases of anemia have been reported in populations of channel catfish Ictalurus punctatus Rafinesque cultured in the southeastern United States. Environmental nitrite-nitrogen concentrations of 4 mg/L or more occur sporadically in channel catfish culture ponds, and the frequency of occurrence is greatest in the fall and spring. The authors have observed that some cases of anemia in populations of pond-raised channel catfish follow prolonged exposure to high concentrations of environmental nitrite. However, there was no evidence that exposure of channel catfish to environmental nitrite was the cause of the observed anemia. Hemolytic anemia following nitrite exposure has been described for sea bass Dicentrarchus labrax (L.) and rainbow trout Salmo gairdneri, but not for channel catfish. In the present study the authors show that a variable, but generally mild, anemia develops in channel catfish exposed to nitrite. They also offer a management procedure for preventing the development of anemia during periods of elevated environmental nitrite concentrations.

  13. Verification of otolith identity used by fisheries scientists for aging channel catfish

    USGS Publications Warehouse

    Long, James M.; Stewart, David R.

    2010-01-01

    Previously published studies of the age estimation of channel catfish Ictalurus punctatus based on otoliths have reported using the sagittae, whereas it is likely they were actually using the lapilli. This confusion may have resulted because in catfishes (ostariophyseans) the lapilli are the largest of the three otoliths, whereas in nonostariophysean fish the sagittae are the largest. Based on (1) scanning electron microscope microphotographs of channel catfish otoliths, (2) X-ray computed tomography scans of a channel catfish head, (3) descriptions of techniques used to removed otoliths from channel catfish reported in the literature, and (4) a sample of channel catfish otoliths received from fisheries biologists from around the country, it is clear that lapilli are most often used for channel catfish aging studies, not sagittae, as has been previously reported. Fisheries scientists who obtain otoliths from channel catfish can use the information in this paper to correctly identify otolith age.

  14. Effect of Dissolved Oxygen Concentration on Development and Hatching of Channel Catfish Ictalurus punctatus Eggs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Recommendations on required dissolved oxygen (DO) concentrations in channel catfish hatcheries vary widely. This study was conducted to determine effects of DO concentration on development and hatching success of channel catfish eggs. Five channel catfish spawns were collected from a pond at the T...

  15. Pre-spawning carotenoid fortified diets improve reproductive traits of channel catfish, Ictalurus punctatus and subsequent progeny performance

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Availability of consistent number of ovulatory competent channel catfish females is a pre-requisite for efficient production of channel catfish ' x blue catfish, I.furcatus ') hybrid in hatcheries. Raising hybrid catfish in production ponds enables the catfish farmer to harness improved growth rate...

  16. Comparative susceptibility of Channel Catfish, Blue Catfish, and their hybrid cross to experimental challenge with Bolbophorus damnificus (Digenea: Bolbophoridae) cercariae.

    PubMed

    Griffin, Matt J; Reichley, Stephen R; Khoo, Lester H; Ware, Cynthia; Greenway, Terrence E; Mischke, Charles C; Wise, David J

    2014-06-01

    The digenetic trematode Bolbophorus damnificus has been implicated in significant losses in catfish aquaculture since the late 1990s. The complex life cycle sequentially involves the American white pelican Pelecanus erythrorhynchos, the marsh rams horn snail Planorbella trivolvis, and Channel Catfish Ictalurus punctatus. Research supports anecdotal reports from the industry, suggesting that the hybrid of Channel Catfish×Blue Catfish I. furcatus is less susceptible to disease agents that have been historically prohibitive to Channel Catfish production, namely the gram-negative bacteria Edwardsiella ictaluri and Flavobacterium columnare, as well as the myxozoan parasite Henneguya ictaluri. This current research compared the susceptibility of Channel Catfish, Blue Catfish, and their hybrid cross to an experimental challenge by B. damnificus. Fish were exposed to 0, 100, 200, and 400 B. damnificus cercariae per fish, and the numbers of metacercariae per fish were determined 14 d postchallenge. Metacercariae were recovered from all challenged fish. There were no significant differences among fish groups challenged with the same dose, suggesting Channel and Blue Catfish and their hybrid are comparably susceptible to B. damnificus infection. As such, it is recommended that producers raising hybrid catfish remain diligent in controlling populations of the snail intermediate host to prevent production losses attributed to B. damnificus, especially when loafing pelicans have been observed at the aquaculture operation. PMID:24895863

  17. Co-culture of channel catfish with hybrid catfish facilitates 'herd effect' to improve production performance

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Herd effect is an epidemiological phenomenon, where the presence or proximity of a certain proportion of improved (superior) individuals improve the performance of less improved (normal) individuals. Channel catfish, Ictalurus puntatus is the single largest aquaculture species cultured in US. Ho...

  18. Comparison of Channel and Blue Catfish Fed Diets Containing Various Levels of Protein

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A comparative study was conducted on growth and protein requirements of channel catfish, Ictalurus punctatus, and blue catfish, Ictalurus furcatus. Four diets containing 24, 28, 32, or 36% protein were fed to both channel (initial weight 6.9 g/fish) and blue (6.6 g/fish) catfish for two growing seas...

  19. Hydrogen peroxide treatments for channel catfish eggs infected with water molds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fungi, or water molds Saprolegnia spp., on channel catfish Ictalurus punctatus eggs can lower fry production. This requires the producer to spawn more catfish or face fingerling shortages. Few treatments have been tested against channel catfish eggs infested with an identified fungus. Hydrogen pe...

  20. Plankton Community Responses in Earthen Channel Catfish Nursery Ponds Under Various Fertilization Regimes

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fertilization recommendations for channel catfish nursery ponds are available, but are not based on systematic research. Current management recommendations for catfish fry production include pond fertilization with cottonseed meal and inorganic fertilizer. We assessed the effects of recommended fe...

  1. Compounds with Inhibitory Activity Against the Channel Catfish Pathogens Edwardsiella ictaluri and Flavobacterium columnare

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Enteric septicemia of catfish (ESC) and columnaris, the most common channel catfish Ictalurus punctatus diseases in the southeastern United States, are caused by the bacteria Edwardsiella ictaluri and Flavobacterium columnare, respectively. Although producers may rely on antibiotics and certain the...

  2. Age, growth, sexual maturity, and food of channel catfish in central Lake Oahe, 1968-69

    USGS Publications Warehouse

    Starostka, Victor J.; Nelson, William R.

    1974-01-01

    Channel catfish, Ictalurus punctatus, were collected with gill nets, trawl, and trap nets at three localities in Lake Oahe for the study of year-class strength, growth in length and weight, age composition, sexual maturity, and food. Fish were recruited to all the collection gears at age II. Relatively strong year classes were produced in 1962, 1965, and 1966. Youngest fish were captured in the upper end of the Moreau River embayment, which apparently serves as a nursery area. Growth of age 0 fish was poor probably because the growing season was short; optimum spawning temperature were not reached until mid-summer. Growth of age II and older fish reached a peak in 1963 and declined rather steadily in 1964-68. Males began to mature at age VIII, and all fish of both sexes were mature at age XI. The diet changed from zooplankton to fish as channel catfish increased in length; aquatic insects (primarily chironomid larvae and pupae) were important foods for fish of all sizes. Fish less than 300 mm long selected large zooplankters -- Leptodora kindtii and Daphnia spp -- over smaller copepods. Larger channel catfish ate principally yellow perch, Perca flavescens.

  3. Virulent Aeromonas hydrophila in channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In this study, we investigated factors that predisposed catfish to motile aeromonas septicemia (MAS) caused by virulent Aeromonas hydrophila (vAh). Our results revealed that wounding on fish body surface was a prerequisite for vAh infection and disease development. A reproducible waterborne challeng...

  4. Rearing channel catfish in a bioflac production system

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Production of channel catfish in an intensively managed mixed suspended-growth, or biofloc, production system was investigated in two studies. Management of unassimilated feed nitrogen is important as feeding rate is increased in intensified production systems. In a mixed suspended-growth production...

  5. Temperature Cycles Induce Early Maturation in Channel Catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A major impediment in improvement of channel catfish by selective breeding is that a high percent of fish do not spawn until the third year. The conditions that lead to sexual maturation in fish have not been established. Size, nutritional state and number of seasonal cycles have all been suggeste...

  6. Delayed feeding of channel catfish fry stocked in ponds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We compared production variables between channel catfish, Ictalurus punctatus, nursery ponds fed according to industry standards, that is feeding immediately at stocking, to an alternative practice of delaying feeding for 6 wk after stocking in an effort to utilize natural pond productivity and redu...

  7. TOXAPHENE: CHRONIC TOXICITY TO FATHEAD MINNOWS AND CHANNEL CATFISH

    EPA Science Inventory

    Fathead minnows (Pimephales promelas) and channel catfish (Ictalurus punctatus) were continuously exposed to several toxaphene concentrations (13-630 ng) in flow-through diluter systems for 8 to 10 months. Growth and backbone quality of adult fathead minnows were decreased at 97 ...

  8. Channel catfish, Ictalurus punctatus, cyclophilin B cDNA sequence

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cyclophilin B is a member of highly conserved immunophilins and ubiquitously found intracellularly. The complete sequence of the channel catfish cyclophilin B cDNA gene consisted of 996 nucleotides. Analysis of the nucleotide sequence reveals one open reading frame and 5’- and 3’-end untranslated...

  9. Characterization of mannose binding lectin from channel catfish Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Mannose-binding lectin (MBL) is an important component of innate immunity capable of activating the lectin pathway of the complement system. A MBL gene was isolated from channel catfish (Ictalurus punctatus). The deduced protein contains a canonical collagen-like domain, a carbohydrate recognition d...

  10. Controlling fungus on channel catfish eggs with peracetic acid

    Technology Transfer Automated Retrieval System (TEKTRAN)

    There is much interest in the use of peracetic acid (PAA) to treat pathogens in aquaculture. It is a relatively new compound and is approved for use in Europe, but not in the United States. This study determined the effectiveness of PAA for fungus control on channel catfish Ictalurus punctatus egg...

  11. Toxicity of Selected Mosquito Sprays to Channel Catfish Sac Fry

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In the spring when channel catfish, Ictalurus punctatus, hatcheries are in full operation, the associated moisture and warm temperatures provide a haven for mosquitoes. Large swarms of biting mosquitoes in a hatchery can make the tedious work of egg-picking (i.e., removing dead and fungus-infested e...

  12. Chemotactic response of Flavobacterium columnare to channel catfish mucus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Research has demonstrated that genomovar II Flavobacterium columnare isolates are more pathogenic for channel catfish (Ictalurus punctatus) and have a higher capacity for adhesion than genomovar I isolates. To begin to define the basis for this, the objectives of the present study were to determine...

  13. Longevity of Bolbophorus damnificus infections in channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The digene, Bolbophorus damnificus infects commercial channel catfish (Ictalurus punctatus) causing mortality, lower feed consumption, and slower or reduced growth in surviving fish. The purpose of this study was to determine the length of time that B. damnificus prodiplostomula metacercariae (juve...

  14. Some Stress Can Improve the Growth of Channel Catfish Fry

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Results from previous studies suggested that channel catfish (Ictalurus punctatus) fry that were housed in very high traffic areas from hatch until reaching 9 g in size were able to survive experimental challenge with Edwardsiella ictaluri better than siblings who were hatched and raised in very low...

  15. Channel Catfish, Ictalurus punctatus, CD63 cDNA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    CD63, also known as lysosome associated membrane protein 3 (LAMP-3), is a member of tetraspanin integral membrane protein family. This protein plays many important roles in immuno-physiological functions. In this communication, we cloned, sequenced and characterized the channel catfish, CD63 transcr...

  16. Temperature Cycles Induce Early Maturation in Channel Catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A major impediment in improvement of channel catfish by selective breeding is that a high percent of fish do not spawn until the third year. If the generation time could be shortened, genetic improvement could be achieved at a faster rate. The conditions that lead to sexual maturation in fish have...

  17. Evaluation of Zooplankton in Hatchery Diets for Channel Catfish Fry

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The efficacy of zooplankton as a supplemental hatchery diet for fry of channel catfish Ictalurus punctatus was evaluated. When a commercial diet is used as a reference, fry fed exclusively on zooplankton–either live or dried–performed poorly in their growth rate. However, when live or dried zooplan...

  18. PROTECTIVE EFFECT OF CUTANEOUS ANTIBODY PRODUCED BY CHANNEL CATFISH IMMUNE TO ICHTHYOPHTHIRIUS ON COHABITED NON-IMMUNE CATFISH

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fish recovered from sublethal ichthyophthiriasis acquire protective immunity against Ichthyophthirius (Ich). This study evaluated the protective effect of cutaneous antibody excreted by channel catfish immune to Ich on cohabited non-immune catfish. Non-immune and immune fish controls were separatel...

  19. First Season Catfish Farming. A Workbook for Beginning Pond and Cage Culture of Channel Catfish. Teacher Edition and Student Edition.

    ERIC Educational Resources Information Center

    Oklahoma State Board of Vocational and Technical Education, Stillwater. Curriculum and Instructional Materials Center.

    This workbook, comprised of both the teacher and student editions, presents guidelines useful for first-year catfish farmers in Oklahoma using pond or cage cultures to raise channel catfish. The teacher edition is a set of unit guidelines only. Contents include a list of suggested readings, important addresses with types of information available…

  20. Making A Better Hybrid Catfish: Can Hybridization of Channel Catfish Selected for ESC Resistance Improve Disease Resistance?

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Improved production traits such as disease resistance and growth have long been realized from the hybridization of channel catfish (Ictalurus punctatus) female X and blue catfish (Ictalurus furcatus) male. Recent advances in hybrid embryo production technology to consistently produce higher number...

  1. Evaluation of supplemental dietary lipids to improve reproductive performance of female channel catfish in hybrid catfish production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Presently, hormone-induced spawning of channel catfish is a viable method for commercial hybrid catfish production. Nutritional status of the broodstock determines the quality of the egg. Lipids can be incorporated into egg yolk from dietary sources, or from reserves that are stored before vitello...

  2. Iron status of channel catfish Ictalurus punctatus affected by channel catfish anemia and response to parenteral iron

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Originally reported in 1983, channel catfish anemia (CCA), also ‘white lip’ or ‘no blood,’ is a major idiopathic disease affecting commercial production in the Mississippi Delta region of the USA. Affected individuals are characterized by lethargy, anorexia, extreme pallor, and packed cell volumes o...

  3. Construction, characterization, expression and immune responses of flagellar proteins of channel catfish, important pathogen Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Background: Edwardsiella ictaluri causes enteric septicemia of catfish, which is the leading disease in channel catfish (Ictalurus punctatus)and is responsible for $50 - 60 million economic losses to catfish producers annually in the southeastern U.S. Bacterial flagella are complex polymeric structu...

  4. Breeding strategy of US farm-raised channel catfish, Ictalurus punctatus affects progeny production and performance

    Technology Transfer Automated Retrieval System (TEKTRAN)

    catfish propagation for decades has been dependent on random mating of male and female channel catfish in ponds. It is simple and has been fairly successful in fulfilling the needs of the US farm-raised catfish industry. However, natural pond spawning is unreliable, unpredictable, and incurs 30 t...

  5. Proximate Composition and Collagen Concentration of Processing Residue of Channel Catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Residues (including heads, skin, viscera, frames, and trimmings) from the channel catfish Ictalurus punctatus processing industry have generally been made into catfish meal and catfish oil that are used in animal feeds. There may be more efficient uses for these materials, such as producing collagen...

  6. Detection and quantification of virulent Aeromonas hydrophila in channel catfish tissues following waterborne challenge

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The aim of this study was to understand the pathogenesis of motile aeromonas septicemia caused by virulent A. hydrophila (vAh) in channel catfish, Ictalurus punctatus. Adipose fin clipped catfish were challenged with vAh using waterborne challenge method and the distribution of vAh in catfish tissue...

  7. Channel catfish Ictalurus punctatus size and feed conversion ratio

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish, Ictalurus punctatus, of five size-classes were stocked into 20, 0.04-ha earthen ponds at a rate of 14,826 fish/ha. Mean initial weights for each size-class were 0.232, 0.458, 0.678, 0.911, and 1.10 kg/fish. Four ponds were randomly allotted to each treatment. A commercial 28% protei...

  8. Disposition of 1-naphthol in the channel catfish (Ictalurus punctatus)

    USGS Publications Warehouse

    Stehly, G.R.; Plakas, S.M.

    1992-01-01

    The pharmacokinetics, tissue distribution, and metabolism of 1-naphthol were examined in the channel catfish (Ictalurus punctatus ). After oral dosing, peak plasma concentrations of 1-naphthol occurred at 1 hr; parent compound made up less than 15% of the total radioactivity, and the bioavailability was 32%. Plasma protein binding was 92% and was independent of concentration. 1-Naphthol and metabolites were rapidly eliminated from the tissues after oral dosing.

  9. Catfishes

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Catfishes are esteemed and popular foodfish throughout much of the world. Important commercial and subsistence fisheries exist in Europe, Asia, Africa, and North and South America. The popularity of catfishes as food stimulated rapid development of catfish aquaculture in the past quarter century. Ap...

  10. Palatability of diets for channel catfish that contain amprolium or salinomycin using feed conversion ratio as criterion

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Two feeding studies were conducted to evaluate the palatability of diets for juvenile Channel Catfish Ictalurus punctatus that contained the poultry anticoccidial drugs Amprolium or Salinomycin, which are candidates to control the Channel Catfish myxozoan parasite, Henneguya ictaluri. These chemothe...

  11. Development and evaluation of an acoustic device to estimate size distribution of channel catfish in commercial ponds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    As one step in the continued effort to utilize acoustic methods and techniques to the betterment of catfish aquaculture, an acoustic “catfish sizer” was designed to determine the size distribution of Channel Catfish Ictalurus punctatus in commercial ponds. The catfish sizer employed a custom-built 4...

  12. Impact of minimum daily dissolved oxygen concentration on performance of hybrid female channel catfish Ictalurus punctatus x male blue catfish I. furcatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Hybrid Catfish (female Channel Catfish Ictalurus punctatus X male Blue Catfish I. furcatus) were reared during two years as single-batch crops under two different dissolved oxygen (DO) regimes each year; a high-DO (control) treatment in which the minimum daily DO was maintained above 3.8 ppm during ...

  13. INFLUENCE OF NATURAL TRICHODINA SP.PARASITISM ON EXPERIMENTAL STREPTOCOCCUS INIAE OR Streptococcus AGALACTIAE INFECTION AND SURVIVAL OF YOUNG CHANNEL CATFISH ICTALURUS PUNCTATUS (RAFINESQUE)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Streptococcus iniae and S. agalactiae are usually not considered pathogens of channel catfish, Ictalurus punctatus, though concurrent infections may decrease catfish survival when infected with streptococcal organisms. Non-parasitized or naturally-parasitized channel catfish fry were challenged wit...

  14. Golden catfish microsatellite analysis reveals a distinct Iinbred stock of channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Golden-colored fish have been reported for rainbow trout, tilapia, several species of carps and Clarias catfish. The current golden catfish stock was created through mixing fish with predominant gold/yellow pigment and spots to normal sized and colored catfish. The golden catfish possess a distinct...

  15. Seasonal Differences in Steroids and Maturation-related Genes in Channel Catfish Under Normal and Accelerated Thermoperiods

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Selective breeding of channel catfish, Ictalurus punctatus, is hampered by a long generation time. Female channel catfish typically spawn when they are 3-years-old; however, a low percentage of spawning may be observed at two years of age. Mature female channel catfish can spawn once annually. Their...

  16. Comprehensive survey and genomic characterization of toll-like receptors in channel catfish, Ictalurus punctatus: identification of novel fish TLRs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A comprehensive survey of channel catfish Toll-Like Receptors (TLRs) was undertaken following a genomic PCR approach based on degenerate primers. Twenty different TLRs were identified in channel catfish. Channel catfish TLR sequences were characterized by phylogenetic analysis based on their conserv...

  17. Chicken-type lysozyme in channel catfish: Expression analysis, lysozyme activity and efficacy as immunostimulant against Aeromonas hydrophila infection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    To understand whether chicken-type lysozyme (Lys-c) in channel catfish was induced by infection of Aeromonas hydrophila, the transcriptional levels of Lys-c in skin, gut, liver, spleen, posterior kidney, and blood cells in healthy channel catfish was compared to that in channel catfish infected with...

  18. Chicken-type lysozyme in channel catfish: expression analysis, lysozyme activity, and efficacy as immunostimulant against Aeromonas hydrophila infection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    To understand whether chicken-type lysozyme (Lys-c) in channel catfish was induced by infection of Aeromonas hydrophila, the transcriptional levels of Lys-c in skin, gut, liver, spleen, posterior kidney, and blood cells in healthy channel catfish was compared to that in channel catfish infected with...

  19. C DNA CLONING, CHARACTERIZATION AND EXPRESSION ANALYSIS OF CHANNEL CATFISH (ICTALURUS PUNCTATUS RAFINESQUE, 1818) PEROXIREDOXIN 6 GENE

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Peroxiredoxin 6 gene (Prdx6) of channel catfish, Ictalurus punctatus, was cloned and sequenced. Total RNA from channel catfish tissues was isolated, reverse transcribed and amplified. The sequence of the channel catfish Prdx6 gene consists of 1003 nucleotides. Analysis of the nucleotide sequence ...

  20. Effect of type and concentration of water hardness on the fertilization and hatching success of channel X blue F1 hybrid catfish eggs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Consistent and improved performance of channel x blue F1 hybrid catfish fingerlings in production ponds in the US farm raised catfish industry has prompted an increase in demand of hybrid catfish fingerlings even at higher prices compared to commonly raised channel catfish. Hybrid catfish fry are e...

  1. Suspending mammalian LHRHa-injected channel catfish, Ictalurus punctatus in individual nylon-mesh bag reduces stress and improves reproductive performance

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Hormone induced spawning of channel catfish held communally in tanks is a reliable method to produce channel catfish, Ictalurus punctatus ' x blue catfish, I. furcatus ' F1 hybrid catfish fry. However, mature catfish are crowded, and repeatedly handled during the process of induced ovulation. Repe...

  2. Effects of winter feeding on growth, body composition, and processing traits of co-cultured blue catfish, channel catfish, and channel catfish x blue catfish hybrids

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Many catfish farmers do not feed or feed fish very infrequently during the winter months because feeding activity is reduced at cold water temperatures. However, studies have demonstrated that winter feeding can improve catfish weight gain and processing yield relative to not feeding. We compared ...

  3. Molecular Cloning and Sequencing of Hemoglobin-Beta Gene of Channel Catfish, Ictalurus Punctatus Rafinesque

    Technology Transfer Automated Retrieval System (TEKTRAN)

    : Hemoglobin-y gene of channel catfish , lctalurus punctatus, was cloned and sequenced . Total RNA from head kidneys was isolated, reverse transcribed and amplified . The sequence of the channel catfish hemoglobin-y gene consists of 600 nucleotides . Analysis of the nucleotide sequence reveals one o...

  4. The use of copper sulfate in channel catfish hatcheries: Safety and effectiveness

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper sulfate (CuSO4) is an economical treatment to control fungus (Saprolegnia spp.) on channel catfish eggs and is widely used by the industry. Several studies were designed to determine the effectiveness and safety of CuSO4 to channel catfish eggs in pursuit of an FDA-approval. Effectiveness:...

  5. Destiny-yield relationship for channel catfish reared in a biofloc technology production system

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The effect of stocking density on yield of stocker channel catfish and water quality in a biofloc technology production system was studied in this completely randomized design experiment. Fingerling channel catfish (Ictalurus punctatus; 48.0 g/fish, 17.8 cm/fish) were stocked into nine continuously ...

  6. Effects of metomindate hydrochloride and tricaine methanesulfonate on the short term cortisol response in channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The effects of metomidate hydrochloride and tricaine methanesulfonate (MS-222) on cortisol stress response of channel catfish, Ictalurus punctatus, were examined during 10 minutes of sedation. Channel catfish were assigned to three treatments: 1. Metomidate hydrochloride (12.5 mg/L), 2. MS-222 (100...

  7. Performance of a temperate-zone channel catfish biofloc technology production system during winter

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish (Ictalurus punctatus) have been grown successfully in an outdoor biofloc technology production system. Outdoor biofloc production systems in the tropics are operated year-round, whereas the channel catfish studies were conducted only during the growing season and biofloc production t...

  8. Biofloc production technology promising in temperate zones – Channel catfish perform during winter months

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A study was conducted to assess the feasibility to grow Channel catfish (Ictalurus punctatus) in an outdoor biofloc system during winter in a temperate zone. High biomasses of market-size channel catfish were successfully maintained through the winter with high survival and in good condition in both...

  9. Evaluation of hydrolyzed poultry feathers as a dietary ingredient for pond-raised channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The present study examined the use of hydrolyzed poultry feathers (HPF) as a replacement for soybean meal in diets for pond raised channel catfish Ictalurus punctatus. Four isonitrogenous (28% crude protein) diets were evaluated that contained 0, 5, 10, or 15% HPF. Fingerling Channel catfish (mean ±...

  10. Passive immunization of channel catfish Ictalurus punctatus with anti-Flavobacterium columnare sera

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Passive immunization of channel catfish, Ictalurus punctatus (Rafinesque), was conducted to determine if anti-Flavobacterium columnare serum was protective when injected intraperitoneally (i.p.) into channel catfish. The anti-F. columnare serum was produced by actively immunizing (i.p. injection) ch...

  11. Comparison of Growth, Body Composition, and Stress Response of Three Select Lines and Industry Channel Catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Improvement in growth and resistance to stress has been identified as traits of interest for genetic improvement. Channel catfish selected solely for fast growth (fast growing) were compared to USDA103, USDA403, and industry pool groups of channel catfish for growth, body composition, and stress re...

  12. The see-saw a vertical-lift incubator designed for channel catfish egg masses

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish egg masses are typically incubated in baskets that are suspended in water that is agitated with rotating or oscillating paddles. We designed and tested a new vertical-lift incubator (the “See-Saw”) to incubate channel catfish egg masses. Preliminary research in commercial hatcheries...

  13. "No evidence for intercohort cannibalism in mixed-size cultures of food-size and fingerling hybrid catfish (channel catfish x blue catfish) grown in ponds in winter or summer."

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Hybrid catfish (' Channel Catfish Ictalurus punctatus X ' Blue Catfish I. furcatus) are normally harvested by seining single-batch pond production systems in fall or winter. Ponds are typically restocked without draining. There is concern that without completely draining the pond after harvest, food...

  14. Sublethal effects of malathion on channel catfish, Ictalurus punctatus

    SciTech Connect

    Areechon, N.; Plumb, J.A. )

    1990-03-01

    Malathion (O,O-dimethyl-S-1, 2-di-(ethoxycarbamyl)ethyl phosphorodithioate) is an organophosphate which is a widely used pesticide in the United States. This pesticide seems to be more toxic to insects and fish than to mammals due to the lack of hydrolytic enzymes in insects and fish. Malathion clearly affects the hematology of fish. The study reported here was designed to determine the effects of a commercially available malathion solution on channel catfish Ictalurus punctatus. Objectives were to measure hematological changes and histopathological effects of a sublethal concentration of malathion on the fish.

  15. BOB.1 of the channel catfish, Ictalurus punctatus: Not a transcriptional coactivator?

    PubMed Central

    Richard, Mara L. Lennard; Hikima, Jun-ichi; Wilson, Melanie R.; Miller, Norman W.; Cunningham, Charles; Warr, Gregory W.

    2010-01-01

    Expression of the immunoglobulin heavy chain (IGH) locus of the channel catfish (Ictalurus punctatus)is driven by the Eμ3′ enhancer, whose core region contains two octamer motifs and a μE5 site. Orthologues of the Oct1 and Oct2 transcription factors have been cloned in the channel catfish and shown to bind to the octamer motifs within the core enhancer. While catfish Oct2 is an activator of transcription, catfish Oct1 failed to drive transcription and may act as a negative regulator of IGH transcription. In mammals, the Oct co-activator BOB.1 (B cell Oct-binding protein1, also known as OCA-B and OBF-1) greatly enhances the transcriptional activity of Oct factors and plays an important role in the development of the immune system. An orthologue of BOB.1 has been cloned in the catfish, and its function characterized. The POU binding domain of the catfish BOB.1 was found to be 95% identical at the amino acid level with the binding domain of human BOB.1, and all the residues directly involved in binding to the Oct-DNA complex were conserved. Despite this conservation, catfish BOB.1 failed to enhance transcriptional activation mediated by endogenous or co-transfected catfish Oct2, and failed to rescue the activity of the inactive catfish Oct1. Electrophoretic mobility shift assays showed that catfish BOB.1 was capable of binding both catfish Oct1 and Oct2 when they formed a complex with the Oct motif. Analysis of recombinant chimeric catfish and human BOB.1 proteins demonstrated that the failure to drive transcription was due to the lack of a functional activation domain within the catfish BOB.1. PMID:19041136

  16. Effects of egg quality and method of incubation on the hatching success of channel X blue hybrid catfish eggs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The objective of this study was to evaluate the effects of egg quality of stripped eggs from channel catfish (Ictalurus punctatus) and method of incubation of fertilized hybrid catfish eggs on hatching success. Stripped eggs from 17 channel catfish females were evaluated in a 2 x 2 factorial...

  17. Bile salts are effective taste stimuli in channel catfish.

    PubMed

    Rolen, S H; Caprio, J

    2008-09-01

    Bile salts are known olfactory stimuli for teleosts, but only a single report has indicated that the taste system of a fish was sensitive to this class of stimuli. Here, gustatory responses of the channel catfish, Ictalurus punctatus, to four bile salts that included taurine-, glycine- and non-conjugated compounds along with three stimulatory amino acids as a comparison were investigated using extracellular electrophysiological techniques. Integrated multiunit responses were obtained from the branch of the facial nerve innervating taste buds on the maxillary barbel. Bile salts were shown to be highly effective facial taste stimuli, with estimated electrophysiological thresholds for three of the four tested bile salts of approximately 10(-11) mol l(-1) to 10(-10) mol l(-1), slightly lower by 1-2 log units than those to amino acids in the same species. Although the sensitivity of the facial taste system of the channel catfish to bile salts is high, the relative magnitude of the response to suprathreshold concentrations of bile salts was significantly less than that to amino acids. Multiunit cross-adaptation experiments indicate that bile salts and amino acids bind to relatively independent receptor sites; however, nerve-twig data and single-fiber recordings suggest that both independent and shared neural pathways exist for the transmission of bile salt and amino acid information to the primary gustatory nucleus of the medulla. PMID:18723536

  18. Learned olfactory discrimination versus innate taste responses to amino acids in channel catfish (Ictalurus punctatus).

    PubMed

    Valentincic, T; Wegert, S; Caprio, J

    1994-05-01

    Intact channel catfish conditioned to the L-amino acids, proline, arginine, alanine, and lysine, discriminated these stimuli from all other amino acids tested. Behavioral structure-activity tests indicated that L-pipecolate was the only effective agonist of the L-proline conditioned response. For channel catfish in which one of the paired olfactory organs was surgically removed, the number of turns to the conditioned stimulus was 40% fewer than those of intact catfish; however, these semiosmic channel catfish discriminated the conditioned from nonconditioned stimuli, as evidenced by their responding to the conditioned amino acid, with a two- to threefold greater number of turns than to the nonconditioned amino acids. Irrespective of the number of conditioning trials attempted, catfish with both olfactory organs removed were unable to discriminate the conditioned from the nonconditioned stimuli. PMID:8022906

  19. Response of channel x blue hybrid catfish to chronic diurnal hypoxia

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Performance traits and metabolic responses of the channel x blue hybrid catfish (Ictalurus punctatus female x I. furcatus male) in response to chronic diurnal hypoxia were evaluated in this 197-d study. Sixteen 0.1-ha earthen ponds were stocked with 15,169 hybrid catfish/ha (47 g/fish) and managed t...

  20. T-cell receptor accessory and co-receptor molecules in channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    T cell receptor (TCR) associated invariant chains CD3gamma/delta,epsilon, and zeta as well as TCR co-receptors CD8alpha and CD8beta were isolated from the channel catfish, Ictalurus punctatus, at both the gene and cDNA levels. All of catfish CD3 sequences encode for proteins that resemble their resp...

  1. Susceptibility of channel catfish, Ictalurus punctatus, to Edwardsiella ictaluri challange following copper sulfate exposure

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish, Ictalurus punctatus (Rafinesque) with or without a preliminary 24h exposure to 2 mg copper sulfate L-1, were challenged with 7.5 X 106 colony forming units L-1 of Edwardsiella ictaluri to determine the effect of copper sulfate on disease resistance. Catfish previously exposed to co...

  2. Efficacy of Ichthyophthirius vaccines in channel catfish against white spot disease

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Ichthyophthirius (Ich) is a protozoan that causes white spot disease in many cultured fish and lead to severe losses in aquaculture. Two trials were conducted to determine the efficacy and serum antibody response of different formulation of Ich vaccines in channel catfish. In trial I, catfish were i...

  3. Identification and Expression Analyses of Poly[I:C]-stimulated Genes in Channel Catfish (Ictalurus punctatus)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish (Ictalurus punctatus) have proven to be an excellent model with which to study immune responses in lower vertebrates. Identification of antiviral antibodies and cytotoxic cells, as well as both type I and II interferon (IFN), demonstrate that catfish likely mount a vigorous anti-vir...

  4. Effect of daily minimum dissolved oxygen concentration on production of channel x blue hybrid catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    As the channel x blue hybrid catfish is stocked by an increasing number of catfish farmers, it is important to quantify the production response of this fish to pond dissolved oxygen management strategies. The purpose of this study was to quantify the production and water quality responses of the cha...

  5. Susceptibility of channel catfish (Ictalurus punctatus) fed dietary sodium chloride to nitrite toxicity

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Juvenile channel catfish (Ictalurus punctatus) were fed nutritionally complete, practical basal diets supplemented with NaCl at 0, 1, 2, or 4 % of diet to apparent satiation twice daily for 10 weeks. Catfish were exposed to nitrite after six (7.70 mg/l nitrite-N) and ten (7.18 mg/l nitrite-N) weeks ...

  6. Effect of threadfin shad as forage for channel catfish fed daily or every third day

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We evaluated whether stocking threadfin shad (Dorosoma petenense) as a forage fish in multiple-batch channel catfish (Ictalurus punctatus) production ponds could substitute for formulated feed when catfish were fed daily or every three days. A completely randomized design in 2 x 2 factorial arrangem...

  7. Genomic Organization of channel catfish, Ictalurus punctatus, matrix metalloproteinase-9-gene

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In the course of studying pathogenesis of enteric septicemia of catfish, we noted that channel catfish matrix metalloproteinase-9 (MMP-9) gene was up-regulated after Edwardsiella ictaluri infection. In this study, we cloned and sequenced MMP-9 genomic DNA by using a Unversal GenomeWalker kit. The co...

  8. Characterization of anti-channel catfish MHC class II monoclonal antibodies

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study characterizes four monoclonal antibodies (mAb) developed against the major histocompatibility complex (MHC) class II beta chain of the channel catfish, Ictalurus punctatus. Immunoprecipitations using catfish clonal B cells revealed that each of these mAbs immunoselected proteins of appro...

  9. ENDOCRINE RESPONSES OF FAST AND SLOW GROWING FAMILIES OF CHANNEL CATFISH ICTALURUS PUNCTATUS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sixty-eight families of USDA303 channel catfish were evaluated for growth performance for 30 days. The four fastest and four slowest growing catfish were further evaluated to examine the hypothesis that genes or gene products associated with the growth regulatory and stress axes could be used to de...

  10. Common off-flavors in pond-raised channel catfish Ictalurus punctatus following partial crop harvest

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish (Ictalurus punctatus) ponds often contain larger standing crops of food-sized catfish than processors can accept at one time. Large fish populations with acceptable flavor quality are partially harvested and remaining fish are returned to the pond and harvested again as soon as poss...

  11. CLONING AND SEQUENCING OF CHANNEL CATFISH (ICTALURUS PUNCTATUS) MATRIX METALLOPROTEINASE-9

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In the course of studying pathogenesis of enteric septicemia of catfish, we noted that channel catfish matrix metalloproteinase-9 (MMP-9) gene was up-regulated after Edwardsiella ictaluri infection. In this study, we cloned and sequenced using the RACE. The complete sequence of the CC MMP-9 cDNA g...

  12. Molecular Cloning, Expression and Genome Organization of Channel Catfish (Ictalurus punctatus) Matrix Metalloproteinase-9

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In the course of studying pathogenesis of enteric septicemia of catfish, we noted that channel catfish matrix metalloproteinase-9 (MMP-9) gene was up-regulated after Edwardsiella ictaluri infection. In this study, we cloned, sequenced using the RACE (rapid amplification of cDNA ends) method and cha...

  13. Molecular Identification of XY Sex-Reversed Female and YY Male Channel Catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Production of channel catfish leads U.S. aquaculture, and monosex culture may provide higher production efficiencies. Determination of phenotypic sex is labor intensive and not practical for large scale culture. Catfish have an X-Y sex determination system with monomorphic sex chromosomes. Hormonal...

  14. Structure, Genomic Organization, and Phylogenetic Implications of Six New VH Families in the Channel Catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    To define members of previously unknown VH gene families, a channel catfish immunoglobulin heavy chain cDNA library was constructed and screened with probes specific for the seven known catfish VH families. Reiterative screening and sequence studies defined six new VH families, designated VH8–VH13, ...

  15. Infection and disease progress of motile Aeromonas septicemia caused by virulent Aeromonas hydrophila in channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Motile Aeromonas septicemia (MAS), caused by virulent clonal isolates of Aeromonas hydrophila (vAh), is emerging as a major disease in channel catfish (Ictalurus punctatus) aquaculture in the Southeastern United States. Predisposing conditions leading to vAh infection in catfish were however largely...

  16. Comparative genomics of Aeromonas hydrophila isolates from an epidemic in channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Background Aeromonas hydrophila was identified as the etiologic agent infecting farmed channel catfish in 2009/2010, resulting in higher mortality rates than typical for motile Aeromonas septicemia with over 5 million pounds of catfish lost to this outbreak. The biochemistry, molecular phylogeny, an...

  17. Expression Profiles of Cloned Channel Catfish (Ictalurus punctatus) Lymphoid Cell Lines and Mixed Lymphocyte Cultures

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Clonal channel catfish lymphoid cell lines and mixed lymphocyte cultures (MLC) have proven extremely useful in examining immune responses at the cellular and molecular levels. To date clonal catfish cell lines and MLC have been biologically and phenotypically characterized using a variety of techniq...

  18. A First Generation Bac-Based Physical Map of the Channel Catfish Genome

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Background: Channel catfish, Ictalurus punctatus, is the leading species in North American aquaculture. Genetic improvement of catfish is performed through selective breeding, and genomic tools will help improve selection efficiency. A physical map is needed to integrate the genetic map with the kar...

  19. Effects of Bio-Mos on growth and survival of channel catfish challenged with Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Research examined the effects of Bio-Mos on growth and disease resistance in channel catfish. Catfish were fed either a Con-Sink (36% crude protein (CP) diet) or Bio-Mos-Sink (36% CP diet with Bio-Mos supplemented at 2 g/kg) sinking pellet for 6 wks. followed by an E. ictaluri challenge. Growth pe...

  20. Improved survival in channel catfish fed mannanoligosaccharides in an extruded diet

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Research examined the effects of extrusion temperature on the efficacy of mannanoligosaccharide (Bio-Mos) in channel catfish grown for 9 wks and then challenged with virulent Edwardsiella ictaluri. Seven hundred and fifty catfish (10.3 ± 0.3 g) were randomly assigned to six treatments with five rep...

  1. Effects of Variable Periods of Food Deprivation on the Development of Enteric septicemia in Channel Catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Enteric septicemia of catfish (ESC), caused by the bacterium Edwardsiella ictaluri, is the most significant bacterial disease affecting channel catfish I. punctatus. Withholding feed during outbreaks of ESC is a widely accepted industry practice used to control losses from the disease. Scientific ev...

  2. Comparative susceptibility of channel catfish, blue catfish, and their hybrid cross to experimental challenge with Bolbophorus damnificus (Digenea: Bolbophoridae) cercariae

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The digenetic trematode Bolbophorus damnificus has been implicated in significant losses in catfish aquaculture since the late 1990s. The complex life cycle sequentially involves the American white pelican Pelecanus erythrorhynchos, the marsh rams horn snail Planorbella trivolvis, and Channel Catfis...

  3. Comparative susceptibility of channel catfish, blue catfish and their hybrid cross to experimental challenge with Bolbophorus damnificus (Digenea: Bolbophoridae) cercariae

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The digenetic trematode Bolbophorus damnificus has been implicated in significant losses in catfish aquaculture since the late 1990s. The complex life cycle sequentially involves the American white pelican Pelecanus erythrorhynchos, the marsh rams horn snail Planorbella trivolvis, and Channel Catfis...

  4. High-density interspecific genetic linkage mapping provides insights into genomic incompatibility between channel catfish and blue catfish.

    PubMed

    Liu, S; Li, Y; Qin, Z; Geng, X; Bao, L; Kaltenboeck, L; Kucuktas, H; Dunham, R; Liu, Z

    2016-02-01

    Catfish is the leading aquaculture species in the United States. The interspecific hybrid catfish produced by mating female channel catfish with male blue catfish outperform both of their parent species in a number of traits. However, mass production of the hybrids has been difficult because of reproductive isolation. Investigations of genome structure and organization of the hybrids provide insights into the genetic basis for maintenance of species divergence in the face of gene flow, thereby helping develop strategies for introgression and efficient production of the hybrids for aquaculture. In this study, we constructed a high-density genetic linkage map using the hybrid catfish system with the catfish 250K SNP array. A total of 26,238 SNPs were mapped to 29 linkage groups, with 12,776 unique marker positions. The linkage map spans approximately 3240 cM with an average intermarker distance of 0.25 cM. A fraction of markers (986 of 12,776) exhibited significant deviation from the expected Mendelian ratio of segregation, and they were clustered in major genomic blocks across 15 LGs, most notably LG9 and LG15. The distorted markers exhibited significant bias for maternal alleles among the backcross progenies, suggesting strong selection against the blue catfish alleles. The clustering of distorted markers within genomic blocks should lend insights into speciation as marked by incompatibilities between the two species. Such findings should also have profound implications for understanding the genomic evolution of closely related species as well as the introgression of hybrid production programs in aquaculture. PMID:26537786

  5. In vivo and in vitro CYP1B mRNA expression in channel catfish.

    PubMed

    Willett, Kristine L; Ganesan, Shobana; Patel, Monali; Metzger, Christine; Quiniou, Sylvie; Waldbieser, Geoff; Scheffler, Brian

    2006-07-01

    Our goal was to study the induction of CYP1B mRNA expression in channel catfish (Ictalurus punctatus). CYP1B belongs to the cytochrome P450 superfamily of genes, is involved in the oxidation of endogenous and exogenous compounds, and could potentially be a useful biomarker in fish for exposure to AhR ligands. The full-length catfish CYP1B cDNA is 2417 nt to the polyA tail and encodes a putative protein of 536 amino acids. It has 67% amino acid similarity to carp and zebrafish CYP1B and 68% similarity to carp CYP1B2. Male channel catfish were collected from three Mississippi Delta sites: Lake Roebuck, Itta Bena; Bee Lake, Thornton; and Sunflower River, Indianola. Total RNA was isolated from wild-caught catfish gill, blood, gonad and liver tissues. Quantitative real-time reverse transcriptase PCR was used to determine relative induction of CYP1B in wild catfish compared to laboratory control and BaP-exposed catfish (20mg/kg i.p. after 4 days). BaP exposure significantly induced CYP1B message in blood, gonad, and liver of laboratory catfish. In these same tissues of wild catfish from sites with relatively low sediment contaminants, CYP1B message was not statistically increased relative to laboratory control catfish. CYP1B transcript abundance was higher in gills compared to other tissues in both laboratory and wild catfish. When primary cultured gill cells were treated with increasing concentrations of BaP, TCDD, and PCBs 77, 126 and 169, CYP1B mRNA was induced more than 10-fold while PCB153 and 4,4'DDT did not cause significant CYP1B induction. Our results suggest that catfish CYP1B is induced by the classic AhR ligands. PMID:16697458

  6. Apparent phosphorus availabilities of selected traditional and alternative feedstuffs for channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A digestibility trial with channel catfish Ictalurus punctatus was conducted to determine apparent availability coefficients (AACs) of phosphorus for selected common feedstuffs: soybean meal, cottonseed meal, wheat middlings, corn gluten feed (CGF), and corn distillers dried grains with solubles (DD...

  7. Short-term feed deprivation alters immune status of surface mucosa in channel catfish (Ictalurus punctatus)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Short-term feed deprivation (or fasting) is a common occurrence in aquacultured fish species whether due to season, production strategies, or disease. In channel catfish (Ictalurus punctatus) fasting impacts susceptibility to several bacterial pathogens including Flavobacterium columnare, the causat...

  8. Chronic pathology and longevity of Drepanocephalus spathans infections in juvenile channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Drepanocephalus spathans (Digenea: Echinostomatidae) is a common parasite of the double-crested cormorant (Phalacrocorax auritus). The cercariae of D. spathans have been shown infective to juvenile channel catfish (Ictalurus punctatus). The developing metacercariae concentrate in the cranial regio...

  9. Comparative genomic analysis of bacteriophages specific to the channel catfish pathogen Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Background: The bacterial pathogen Edwardsiella ictaluri is a primary cause of mortality in channel catfish raised commercially in aquaculture farms. Additional treatment and diagnostic regimes are needed for this enteric pathogen, motivating the discovery and characterization of bacteriophages spe...

  10. Effects of Feeding Diets With or Without Fish Meal on Production of Channel Catfish, Ictalurus Punctatus, Stocked at Varying Densities

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Animal protein, generally fish meal, has traditionally been used in the diet of channel catfish. However, our previous research indicates that animal protein is not needed for growing stocker size catfish to food fish when the fish are stocked at densities typical of those used in commercial catfish...

  11. Intracranial and lavage mode of LHRHa administration improves ovulation and spawning performance of channel catfish Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Increased production of channel x blue F1 hybrid catfish fry production and their subsequent performance has advanced their adoption of hybrid catfish among the US catfish farmers. The use of hormone induction as a reproductive strategy to produce hybrid fry has been successfully applied. The aim o...

  12. Changes of serum myeloperoxidase and nitric oxide in the early stage of Edwardsiella ictaluri infection in channel catfish, Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Enteric septicemia of catfish (ESC), caused by Edwardsiella ictaluri, is an important farm-raised channel catfish, Ictalurus punctatus (Rafinesque), disease. The development of a monitoring system for assessing the catfish health status in hatcheries and ponds is in great demanding. Because of the...

  13. Efficacy of hydrogen peroxide for treating saprolegniasis in channel catfish

    USGS Publications Warehouse

    Howe, G.E.; Gingerich, W.H.; Dawson, V.K.; Olson, J.J.

    1999-01-01

    Hatchery-reared fish and their eggs are commonly afflicted with saprolegniasis, a fungal disease that can cause significant losses in production. Fish culturists need safe and effective fungicides to minimize losses and meet production demands. The efficacy of hydrogen peroxide was evaluated for preventing or controlling mortality associated with saprolegniasis in channel catfish Ictalurus punctatus. Saprolegniasis was systematically induced in channel catfish so various therapies could be evaluated in a controlled laboratory environment. Both prophylactic and therapeutic hydrogen peroxide bath treatments of 50, 100, and 150 ??L/L for 1 h were administered every other day for seven total treatments. All untreated positive control fish died of saprolegniasis during the prophylactic and therapeutic tests. Hydrogen peroxide treatments of 150 ??L/L were harmful (relative to lower concentrations) to test fish and resulted in 73-95% mortality. Mortality was attributed to a combination of abrasion, temperature, chemical treatment, and disease stressors. Treatments of 100 ??L/L were less harmful (relatively) but also appeared to contribute to mortality (60-79%). These treatments, however, significantly reduced the incidence of mortality and infection compared with those observed for fish of the positive control or 150-??L/L treatment groups. Overall, treatments of 50 ??L/L were found to be the most safe and effective of those tested. Mortality with this concentration ranged from 16% in therapeutic tests to 41% in prophylactic tests. The statistical model employed estimated that the optimum treatment concentration for preventing or controlling mortality, reducing the incidence of infections, and enhancing the recovery of infected fish was 75 ??L H2O2/L.

  14. Vaccination of full-sib channel catfish families against enteric septicemia of catfish with an oral live attenuated Edwardsiella ictaluri vaccine

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The study evaluated the efficacy of an oral live-attenuated Edwardsiella ictaluri vaccine against enteric septicemia of catfish in 20 full-sib fingerling channel catfish families. Each family was split into vaccinated and non-vaccinated groups. The vaccine was delivered orally by feeding fish diet...

  15. Utilization of a rapid DNA-based assay for molecular verification of channel catfish, blue catfish, F1 hybrid, and backcross offspring at serveral life stages

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The F1 hybrid offspring of channel catfish, Ictalurus punctatus, females mated with blue catfish, I. furcatus, males contain many desirable traits for commercial production such as enhanced growth and increased survivability. Although at a low efficiency, hybrids can be produced by pond spawning, b...

  16. Channel catfish polyculture with fathead minnows or threadfin shad effects on pond plankton communities and catfish fillet flavor, color, and fatty acid composition

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Threadfin shad, Dorosoma petenense, or fathead minnows, Pimephales promelas, were co-cultured with channel catfish, Ictalurus punctatus, in earthen ponds to determine the effects of planktivory on plankton community dynamics and catfish fillet quality. Fathead minnows had no effect on the plankton c...

  17. Hormonal control of osmoregulation in the channel catfish Ictalurus punctatus.

    PubMed

    Eckert, S M; Yada, T; Shepherd, B S; Stetson, M H; Hirano, T; Grau, E G

    2001-06-01

    Prolactin (PRL) is an important hormone for freshwater adaptation in many teleost species. In some euryhaline fishes, growth hormone (GH) and cortisol are involved in seawater adaptation by stimulating ion extrusion. When channel catfish (Ictalurus punctatus) were transferred from fresh water to dilute seawater (300-400 mOsm), their plasma osmolality was always higher than the environmental salinity. In correlation with the increase in plasma osmolality, significant increases in plasma cortisol were observed. However, no effect of ovine GH or cortisol was seen in plasma osmolality or gill Na, K-ATPase activity when the hormones were given during the course of acclimation to dilute seawater. When catfish in fresh water were hypophysectomized, plasma osmolality was significantly decreased by 24 h, reaching a minimum level after 2 days. When they were transferred to dilute seawater, the plasma osmolality of the sham-operated fish was consistently higher than that of environmental water, whereas the osmolality of the hypophysectomized fish was equivalent to the environmental salinity. Ovine PRL restored the plasma osmolality of the hypophysectomized fish in fresh water to the level of sham-operated fish. Cortisol was also effective, but the effect was less pronounced than the effect of PRL. Injection of PRL in combination with cortisol resulted in a marked additive increase in plasma osmolality to a level even above that of the sham-operated fish. Ovine GH was without effect. These treatments in hypophysectomized fish transferred to dilute seawater produced essentially the same results as those in fish in fresh water. Plasma osmolality was also increased after PRL treatment of the intact fish in fresh water. There was a synergistic effect between PRL and cortisol in hypophysectomized fish in dilute seawater as well as in intact fish in fresh water. PRL did not stimulate cortisol secretion either in hypophysectomized fish or in intact fish. In the stenohaline catfish

  18. SEASONAL FORAGING BY CHANNEL CATFISH ON TERRESTRIALLY BURROWING CRAYFISH IN A FLOODPLAIN-RIVER ECOSYSTEM

    EPA Science Inventory

    The seasonal use of terrestrially burrowing crayfish as a food item by channel catfish Ictalurus punctatus was studied in channelized and non-channelized sections of the Yockanookany River (Mississippi, USA). During seasonal inundation of the floodplains, the crayfish occupied o...

  19. Passive immunization of channel catfish Ictalurus punctatus with anti-Flavobacterium columnare sera.

    PubMed

    Shelby, R A; Shoemaker, C A; Klesius, P H

    2007-09-14

    Passive immunization of channel catfish Ictalurus punctatus (Rafinesque) was conducted to determine if anti-Flavobacterium columnare serum was protective when injected intraperitoneally (i.p.) into channel catfish. The anti-F. columnare serum was produced by actively immunizing (i.p. injection) channel catfish with sonicated whole cells or purified lipopolysaccharide (LPS) of F. columnare in Freund's adjuvant. Serum anti-F. columnare activity was verified by Western blotting and ELISA of serum. Normal serum and sterile culture broth were used as controls. Complement was inactivated in all sera by heating. After 48 h, passively immunized fish were challenged with virulent F. columnare by i.p. injection. A group of unchallenged fish served as controls. The immune response of catfish to the antigenic fractions was different when examined by Western blotting. Antibody produced with whole-cell antigen responded to a broad range of molecular weight components, while LPS antigens were restricted to a pair of bands near 20 kDa. Control fish injected with culture medium experienced 100% mortality 14 d post-challenge. Relative percent survival was 77 and 73 for catfish passively immunized with anti-LPS and anti-whole-cell serum, respectively. Results suggest that antibodies in the serum are involved in the protective immune response against columnaris disease in channel catfish. PMID:17972756

  20. Influence of fertilizing water pH on the hatching success of stripped channel catfish eggs on channel x blue hybrid catfish embryo production in hatcheries

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Variable egg quality is one of the most important constrains to the development of aquaculture. The quality of eggs that are manually stripped from channel catfish are affected by variation in parental genetics, maturity, type and dose of hormone, age and pre-spawning stress of female fish. Furthe...

  1. Growth and Feed Efficiency of Channel × Blue Catfish Hybrids Stocked at Various Densities and Fed Once or Twice Daily in Ponds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Two experiments were conducted to evaluate the effects of stocking density and feeding frequency on the growth, net yield, and feed conversion of hybrid catfish (female channel catfish Ictalurus punctatus x male blue catfish I. furcatus). In experiment 1, hybrid catfish fingerlings with a mean initi...

  2. Effect of paternal blue catfish strain effects on hatchery fry production and performance of channel catfish X blue catfish F1 hybrid fry production and fingerling performance under commercial conditions

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Four hundred four-year old mature Gold Kist strain channel catfish, Ictalurus punctatus were induced to spawn with 10 mg common carp pituitary extract (CPE) /kg BW in 8 spawning trials. The stripped eggs were fertilized with sperm of either D&B or Rio Grande blue catfish, I.furcatus to produce D&B ...

  3. Elicitation of macrophages from the peritoneal cavity of channel catfish

    USGS Publications Warehouse

    Jenkins, J.A.; Klesius, P.H.

    1998-01-01

    Four chemicals were evaluated for elicitation of macrophages in peritoneal cavities of 250-300g healthy channel catfish Ictalurus punctatus. Cellular exudates were collected at 3, 5, 7, 10, 14, and 20 d following intraperitoneal injections with squalene, Freund's incomplete adjuvant (FIA), goat serum, thioglycollate, or as a control, phosphate-buffered saline. Injection with either squalene or FIA induced significantly greater (P ??? 0.0001) macrophage recruitment than the other chemicals. The effectiveness of squalene and FIA was compared further by macrophage collection daily for 7 d. Squalene and FIA elicited similarly high macrophage responses (P ??? 0.0450), the highest being 3.43 x 106 macrophages/mL (SE, 2.4 x l06) at 99% purity at day 2 and 2.1 X 106 macrophages/mL (SE, 0.7 x 106) at day 14 at 80% purity, respectively. In both experiments, the time after injection was not statistically significant, nor was there an interaction between time and chemicals. The occurrence of cells other than macrophages decreased with time to yield macrophage recoveries of 47-99% for squalene and 30-80% for FIA. Two subsets of macrophages were observed by means of flow cytometry. As demonstrated by chemiluminescence, the squalene-elicited cells produced high-energy oxygen compounds important to the phagocytic process.

  4. A standardized sampling protocol for channel catfish in prairie streams

    USGS Publications Warehouse

    Vokoun, Jason C.; Rabeni, Charles F.

    2001-01-01

    Three alternative gears—an AC electrofishing raft, bankpoles, and a 15-hoop-net set—were used in a standardized manner to sample channel catfish Ictalurus punctatus in three prairie streams of varying size in three seasons. We compared these gears as to time required per sample, size selectivity, mean catch per unit effort (CPUE) among months, mean CPUE within months, effect of fluctuating stream stage, and sensitivity to population size. According to these comparisons, the 15-hoop-net set used during stable water levels in October had the most desirable characteristics. Using our catch data, we estimated the precision of CPUE and size structure by varying sample sizes for the 15-hoop-net set. We recommend that 11–15 repetitions of the 15-hoop-net set be used for most management activities. This standardized basic unit of effort will increase the precision of estimates and allow better comparisons among samples as well as increased confidence in management decisions.

  5. Thidiazuron uptake, distribution and metabolism in bluegills and channel catfish.

    PubMed

    Knowles, C O; Benezet, H J; Mayer, F L

    1980-01-01

    Bluegills (Lepomis macrochirus) exposed to 0.1 ppm of thidiazuron-14C cotton defoliant for 28 days under continuous flow conditions accumulated relatively low levels of radiocarbon. The maximum detected was 5.4 ppm in fillet tissue after 1 day. During a 14 day depuration period, radioactivity declined to 1.0 ppm or less. Fractionation of offal and fillet tissues from bluegills collected at 28 days indicated that most of the radioactive material was water soluble, although appreciable amounts of organosoluble radioactive material also were present. When bluegills were injected intraperitoneally with thidiazuron-14C, metabolism and elimination were relatively rapid. Organosoluble radioactive material isolated from fish tissue included thidiazuron, its 2-hydroxyphenyl derivative, phenylurea, and several unknowns. Channel catfish (Ictalurus punctatus) exposed under static conditions to a system containing 0.15 ppm of thidiazuron-14C incorporated into soil also accumulated only low concentrations of radiocarbon. The maximum detected was 2.5 ppb in offal tissue at 7 days. In fillet tissue, radioactivity did not exceed 0.5 ppb. There was no evidence from these studies to indicate that thidiazuron would pose a hazard to the aquatic ecosystem. PMID:7400538

  6. The efficacy of mass-marking channel catfish fingerlings by immersion in oxytetracycline

    USGS Publications Warehouse

    Stewart, David R.

    2011-01-01

    Oxytetracycline (OTC) has been extensively used for marking a variety of fish species, but has never been successfully used to mark channel catfish Ictalurus punctatus. Channel catfish fingerlings (~ 25 mm TL) obtained from the Oklahoma Department of Wildlife Conservation at Byron Fish Hatchery were kept in Living Streams (791 to 1,018 L) equipped with recirculation units. Marking trials consisted of immersing channel catfish in one of three concentrations (250, 450, and 700 mg/L) OTC hydrochloride [HCl] for 6 hours. Samples of channel catfish were obtained from each group at 1-week and 4-week postimmersion. Lapilli otoliths and pectoral spines were removed to assess mark presence with an epi-fluorescent compound microscope. After one week, no marks were detected on pectoral spines for all treatments, mark detection on otoliths depended on concentration, but never exceeded 43% (700 mg/L). After four weeks, all otoliths and pectoral spines were determined marked for 700 mg/L OTC, 20% for fish immersed in 450 mg/L OTC, and 0% were marked after four weeks at the 250 mg/L OTC. Results show, channel catfish fingerlings can be successfully marked with immersion in OTC at 700 mg/L for at least 6 hours.

  7. Oral Vaccination of Channel Catfish against Enteric Septicemia of Catfish Using a Live Attenuated Edwardsiella ictaluri Isolate.

    PubMed

    Wise, David J; Greenway, Terrence E; Byars, Todd S; Griffin, Matt J; Khoo, Lester H

    2015-06-01

    Enteric septicemia of catfish (ESC), caused by Edwardsiella ictaluri, is the most problematic bacterial disease affecting catfish aquaculture in the southeastern United States. Efforts to develop an effective ESC vaccine have had limited industrial success. In commercial settings, ESC vaccines are typically administered by immersion when fry are transferred from the hatchery to rearing ponds. While this approach is a practical method of mass delivery, this strategy administers vaccines to very young fish, which lack a fully developed immune system. To circumvent this limitation, an oral vaccination strategy was evaluated as a means of immunizing catfish at the fingerling stage of production, when fish possess a more complete immune arsenal. A virulent E. ictaluri isolate (S97-773) was attenuated by successive passage on media containing increasing concentrations of rifamycin. In laboratory trials, cultured vaccine was diluted and mixed with feed (100 mL diluted vaccine/454 g feed). This mixture was then fed to Channel Catfish Ictalurus punctatus fingerlings. Two separate dilutions of cultured vaccine (1:10 and 1:100) were used to create the vaccine-feed mixture, equating to estimated doses of 5 × 10⁷ and 5 × 10⁶ CFU/g of feed, respectively. After 30 d, catfish were exposed by immersion (1 × 10⁶ CFU/mL) to the virulent parental strain of E. ictaluri. The target dose (1:100 dilution, ∼5 × 10⁶ CFU/g of feed) offered exceptional protection (relative percent survival = 82.6-100%). In addition, negligible deaths occurred in fish vaccinated at 10 times the target dose (1:10 dilution, ∼5 × 10⁷ CFU/g of feed). In pond trials, antibody production increased 18-fold in orally vaccinated fish. When compared with nonvaccinated controls, vaccination significantly improved survival, feed fed, feed conversion, biomass produced, and total harvest. This research demonstrates Channel Catfish can be successfully immunized in a commercial setting against E. ictaluri

  8. Effect of channel catfish stocking rate on yield and water quality in an intensive, mixed suspended-growth production system

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study was conducted to determine the effect of channel catfish (Ictalurus punctatus) stocking rate on yield and water quality in a mixed suspended-growth production system (bio-floc) with zero water exchange. Channel catfish (National Warmwater Aquaculture Center 103 strain; average weight = 13...

  9. The “See-Saw”: A Vertical-Lift Incubator Designed for Channel Catfish Ictalurus punctatus Egg Masses

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish (Ictalurus punctatus) egg masses are typically incubated in baskets that are suspended within metal troughs with water that is agitated and pushed around the eggs with paddles. A limiting factor in the successful incubation of channel catfish eggs is the absorption of oxygen to the ...

  10. Evaluation of corn distillers dried grains with solubles and brewers yeast in diets for channel catfish Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A study was conducted to examine the use of distillers grains with solubles (DDGS), ethanol extracted DDGS (EDDGS), and brewers yeast in channel catfish, Ictalurus punctatus, diets. Diets containing these ingredients were compared with all-plant and fish meal control diets. Juvenile channel catfish ...

  11. Apolipoprotein A1 in channel catfish: Transcriptional analysis, antimicrobial activity, and efficacy as plasmid DNA immunostimulant against Aeromonas hydrophila infection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The objectives of this study were to: 1) determine transcriptional profiles of apolipoprotein A1 (ApoA1) in collected channel catfish tissues after infection with A. hydrophila by bath immersion; 2) investigate whether recombinant channel catfish apolipoprotein A1 produced in E. coli expression syst...

  12. Efficacy of metomidate and tricaine methanesulfonate to modulate the short-term cortisol stress response in channel catfish Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The ability of the anesthetics metomidate and tricaine methanesulfonate to mitigate the cortisol stress response of channel catfish Ictalurus punctatus was evaluated during a 10 min confinement stress. Channel catfish (11.9 ± 0.5 g; mean ± SE) were transferred from holding tanks to confinement buck...

  13. Recombinant goose-type lysozyme in channel catfish: lysozyme activity and efficacy as plasmid DNA immunostimulant against Aeromonas hydrophila infection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The objectives of this study were: 1) to investigate whether recombinant channel catfish lysozyme g (CC-Lys-g) produced in E. coli expression system possesses any lysozyme activity; and 2) to evaluate whether channel catfish lysozyme g plasmid DNA could be used as an immunostimulant to protect chann...

  14. Recombinant goose-type lysozyme in channel catfish: Lysozyme activity and efficacy as plasmid DNA immunostimulant against Aeromonas hydrophila infection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The objectives of this study were: 1) to investigate whether recombinant channel catfish lysozyme g (CC-Lys-g) produced in E. coli expression system possesses any lysozyme activity; and 2) to evaluate whether channel catfish lysozyme g plasmid DNA could be used as an immunostimulant to protect chann...

  15. Channel catfish hatchery production efficiency using a vertical-lift incubator the see-saw at various egg loading densities

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish spawns are typically incubated in ¼-in mesh baskets suspended in water that is agitated with paddles positioned between baskets. We tested a new vertical-lift incubator (the “See-Saw”) to incubate channel catfish spawns. Previous research demonstrated that when loaded with spawns at...

  16. Effects of Various Corn Distillers By-products on Growth and Feed Efficiency of Channel Catfish, Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A study was conducted to examine the use of corn distillers by-products in diets and the effects of additional dietary fat on channel catfish, Ictalurus punctatus, performance. Juvenile channel catfish (initial weight: 12.6 g per fish) were stocked in flow-through aquaria and fed one of six practica...

  17. DNAk is a dominant epitope in the humoral immune response of channel catfish (Ictalurus punctatus) to Flavobacterium columnare

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Vaccination remains a viable alternative for bacterial disease protection in fish; however additional work is required to understand the mechanisms of adaptive immunity in the channel catfish. To assess the humoral immune response to Flavobacterium columnare; a group of channel catfish were first im...

  18. The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.

    PubMed

    Liu, Zhanjiang; Liu, Shikai; Yao, Jun; Bao, Lisui; Zhang, Jiaren; Li, Yun; Jiang, Chen; Sun, Luyang; Wang, Ruijia; Zhang, Yu; Zhou, Tao; Zeng, Qifan; Fu, Qiang; Gao, Sen; Li, Ning; Koren, Sergey; Jiang, Yanliang; Zimin, Aleksey; Xu, Peng; Phillippy, Adam M; Geng, Xin; Song, Lin; Sun, Fanyue; Li, Chao; Wang, Xiaozhu; Chen, Ailu; Jin, Yulin; Yuan, Zihao; Yang, Yujia; Tan, Suxu; Peatman, Eric; Lu, Jianguo; Qin, Zhenkui; Dunham, Rex; Li, Zhaoxia; Sonstegard, Tad; Feng, Jianbin; Danzmann, Roy G; Schroeder, Steven; Scheffler, Brian; Duke, Mary V; Ballard, Linda; Kucuktas, Huseyin; Kaltenboeck, Ludmilla; Liu, Haixia; Armbruster, Jonathan; Xie, Yangjie; Kirby, Mona L; Tian, Yi; Flanagan, Mary Elizabeth; Mu, Weijie; Waldbieser, Geoffrey C

    2016-01-01

    Catfish represent 12% of teleost or 6.3% of all vertebrate species, and are of enormous economic value. Here we report a high-quality reference genome sequence of channel catfish (Ictalurus punctatus), the major aquaculture species in the US. The reference genome sequence was validated by genetic mapping of 54,000 SNPs, and annotated with 26,661 predicted protein-coding genes. Through comparative analysis of genomes and transcriptomes of scaled and scaleless fish and scale regeneration experiments, we address the genomic basis for the most striking physical characteristic of catfish, the evolutionary loss of scales and provide evidence that lack of secretory calcium-binding phosphoproteins accounts for the evolutionary loss of scales in catfish. The channel catfish reference genome sequence, along with two additional genome sequences and transcriptomes of scaled catfishes, provide crucial resources for evolutionary and biological studies. This work also demonstrates the power of comparative subtraction of candidate genes for traits of structural significance. PMID:27249958

  19. The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts

    PubMed Central

    Liu, Zhanjiang; Liu, Shikai; Yao, Jun; Bao, Lisui; Zhang, Jiaren; Li, Yun; Jiang, Chen; Sun, Luyang; Wang, Ruijia; Zhang, Yu; Zhou, Tao; Zeng, Qifan; Fu, Qiang; Gao, Sen; Li, Ning; Koren, Sergey; Jiang, Yanliang; Zimin, Aleksey; Xu, Peng; Phillippy, Adam M.; Geng, Xin; Song, Lin; Sun, Fanyue; Li, Chao; Wang, Xiaozhu; Chen, Ailu; Jin, Yulin; Yuan, Zihao; Yang, Yujia; Tan, Suxu; Peatman, Eric; Lu, Jianguo; Qin, Zhenkui; Dunham, Rex; Li, Zhaoxia; Sonstegard, Tad; Feng, Jianbin; Danzmann, Roy G.; Schroeder, Steven; Scheffler, Brian; Duke, Mary V.; Ballard, Linda; Kucuktas, Huseyin; Kaltenboeck, Ludmilla; Liu, Haixia; Armbruster, Jonathan; Xie, Yangjie; Kirby, Mona L.; Tian, Yi; Flanagan, Mary Elizabeth; Mu, Weijie; Waldbieser, Geoffrey C.

    2016-01-01

    Catfish represent 12% of teleost or 6.3% of all vertebrate species, and are of enormous economic value. Here we report a high-quality reference genome sequence of channel catfish (Ictalurus punctatus), the major aquaculture species in the US. The reference genome sequence was validated by genetic mapping of 54,000 SNPs, and annotated with 26,661 predicted protein-coding genes. Through comparative analysis of genomes and transcriptomes of scaled and scaleless fish and scale regeneration experiments, we address the genomic basis for the most striking physical characteristic of catfish, the evolutionary loss of scales and provide evidence that lack of secretory calcium-binding phosphoproteins accounts for the evolutionary loss of scales in catfish. The channel catfish reference genome sequence, along with two additional genome sequences and transcriptomes of scaled catfishes, provide crucial resources for evolutionary and biological studies. This work also demonstrates the power of comparative subtraction of candidate genes for traits of structural significance. PMID:27249958

  20. Early innate immune response of immune proteins in juvenile channel catfish Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish (Ictalurus punctatus) are raised for aquaculture in the Southeast U.S. and are susceptible to bacterial and viral infections acquired from their pond environment. Innate immune proteins mannose-binding lectin (MBL) and lysozyme were studied during two consecutive years in channel cat...

  1. A first generation BAC-based physical map of the channel catfish genome

    PubMed Central

    Quiniou, Sylvie M-A; Waldbieser, Geoffrey C; Duke, Mary V

    2007-01-01

    Background Channel catfish, Ictalurus punctatus, is the leading species in North American aquaculture. Genetic improvement of catfish is performed through selective breeding, and genomic tools will help improve selection efficiency. A physical map is needed to integrate the genetic map with the karyotype and to support fine mapping of phenotypic trait alleles such as Quantitative Trait Loci (QTL) and the effective positional cloning of genes. Results A genome-wide physical map of the channel catfish was constructed by High-Information-Content Fingerprinting (HICF) of 46,548 Bacterial Artificial Chromosomes (BAC) clones using the SNaPshot technique. The clones were assembled into contigs with FPC software. The resulting assembly contained 1,782 contigs and covered an estimated physical length of 0.93 Gb. The validity of the assembly was demonstrated by 1) anchoring 19 of the largest contigs to the microsatellite linkage map 2) comparing the assembly of a multi-gene family to Restriction Fragment Length Polymorphism (RFLP) patterns seen in Southern blots, and 3) contig sequencing. Conclusion This is the first physical map for channel catfish. The HICF technique allowed the project to be finished with a limited amount of human resource in a high throughput manner. This physical map will greatly facilitate the detailed study of many different genomic regions in channel catfish, and the positional cloning of genes controlling economically important production traits. PMID:17284319

  2. Seasonal migration and homing of channel catfish in the lower Wisconsin River, Wisconsin

    USGS Publications Warehouse

    Pellett, Thomas D.; Van Dyck, Gene J.; Adams, Jean V.

    1998-01-01

    A multiyear tag and recapture study was conducted to determine whether channel catfishIctalurus punctatus were migratory and if they had strong homing tendencies. Over 10,000 channel catfish were tagged from the lower Wisconsin River and adjacent waters of the upper Mississippi River during the 3-year sampling period. Data on movements were obtained from study recaptures and through tag returns and harvest information provided by sport anglers and commercial fishers. Channel catfish occupied relatively small home ranges during summer, migrated downstream to the upper Mississippi River in autumn, then migrated back up the Wisconsin River in spring to spawn and to occupy the same summer home sites they had used in previous summers. Fish size was a factor in the degree of fidelity to summer home sites, with larger fish showing greater fidelity.

  3. Efficacy of formalin and hydrogen peroxide to increase survival of channel catfish infected with saprolegniasis

    USGS Publications Warehouse

    Rach, J.J.; Schreier, T.; Gaikowski, M.P.; Schleis, S.M.

    2005-01-01

    The efficacy of formalin and hydrogen peroxide to increase survival associated with external saprolegniasis on fingerlings of channel catfish Ictalurus punctatus was evaluated in four laboratory trials. Fungal epizootics were initiated according to procedures developed in a fungal-disease model. Fish were abraded with a dremel tool and then placed in an aerated static infection tank containing fungal-infected hemp seeds held in teaballs that were suspended in 100 L of water for 24 h (trials 1-3) or 6 h (trial 4). In the formalin trials, treatment regimens of 0, 82, and 166 mg/L (trial 1) or 0 and 83 mg/L (trial 2) were administered to channel catfish for 60 min once every other day for a total of three treatments (trial 1) or once daily for a total of seven treatments (trial 2). In the hydrogen peroxide trials, treatment regimens of 0, 75, and 102 mg/L (trial 3) or 0 and 75 mg/L (trial 4) were administered to channel catfish for 60 min once every other day for a total of three treatments. Each treatment regimen was tested in triplicate, with 10 fish per replicate. Formalin exposures of 83 (seven treatments; 33% survival) and 166 mg/L (three treatments; 63% survival) for 60 min significantly (P <= 0.01) increased channel catfish survival in comparison with controls (of which 3% and 13%, respectively, survived). In trial 4, hydrogen peroxide exposure of 75 mg/L (63% survival) for 60 min significantly (P <= 0.01) increased channel catfish survival in comparison with the untreated controls (13% survival). Formalin and hydrogen peroxide treatments were effective in increasing survival in channel catfish infected with fungus.

  4. Suspended spawning cans for channel catfish in a surface-mine lake. [Ictalurus punctatus

    SciTech Connect

    Moy, P.B.; Stickney, R.R.

    1987-01-01

    A technique was developed to enable channel catfish (Ictalurus punctatus) to spawn in lakes where sufficient spawning habitat is not available. Forty-five-liter spawning cans were suspended from styrofoam floats at a depth of 1.25 m below the water surface, in water that was 2-5 m deep. Brood fish successfully utilized the containers for spawning, and the presence of eggs in sunken containers suggests that channel catfish will spawn successfully at depths of up to 5 m if satisfactory nesting sites are available.

  5. Some enzymatic properties of brain Acetylcholinesterase from bluegill and channel catfish

    USGS Publications Warehouse

    Hogan, James W.; Knowles, Charles O.

    1968-01-01

    Using a manometric technique an acetylcholinesterase (EC 3.1.1.7, acetylcholine acetyl-hydrolase) was demonstrated in brain tissue from the bluegill, Lepomis macrochirus Rafinesque, and the channel catfish, Ictalurus punctatus (Walbaum). The activities were 19 and 37 μmoles acetylcholine hydrolyzed/milligram protein per hour for the bluegill and channel catfish enzymes, respectively. The optimum substrate concentration for the hydrolysis of acetylcholine was 10 mMfor the enzymes from both species. Generally, the catfish acetylcholinesterase was somewhat more susceptible than the bluegill to the inhibitors tested; however, the bluegill enzyme was more susceptible to inhibition by malathion and malaoxon.

  6. Mucosal expression signatures of two Cathepsin L in channel catfish (Ictalurus punctatus) following bacterial challenge.

    PubMed

    Wang, Renjie; Song, Lin; Su, Baofeng; Zhao, Honggang; Zhang, Dongdong; Peatman, Eric; Li, Chao

    2015-11-01

    The mucosal surfaces of fish are the first line of host defense against various pathogens. The mucosal immune responses are the most critical events to prevent pathogen attachment and invasion. Cathepsins are a group of peptidases that involved in different levels of immune responses, but the knowledge of the roles of Cathepsin in mucosal immune responses against bacterial infection are still lacking. Therefore, in the present study we characterized the Cathepsin L gene family in channel catfish, and profiled their expression levels after challenging with two different Gram-negative bacterial pathogens. Here, two Cathepsin L genes were identified from channel catfish and were designated CTSL1a and CTSL.1. Comparing to other fish species, the catfish CTSL genes are highly conserved in their structural features. Phylogenetic analysis was conducted to confirm the identification of CTSL genes. Expression analysis revealed that the CTSL genes were ubiquitously expressed in all tested tissues. Following infection, the CTSL genes were significantly induced at most timepoints in mucosal tissues. But the expression patterns varied depending on both pathogen and tissue types, suggesting that CTSL genes may exert disparate functions or exhibit distinct tissue-selective roles in mucosal immune responses. Our findings here, clearly revealed the key roles of CTSL in catfish mucosal immunity; however, further studies are needed to expand functional characterization and examine whether CTSL may also play additional physiological roles in catfish mucosal tissues. PMID:26434716

  7. The safety and effectiveness of CuSO4 to control fungus on intact egg masses in channel catfish hatcheries

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper sulfate (CuSO4) is widely used by the catfish industry as an economical treatment to control fungus (Saprolegnia spp.) on channel catfish eggs. This is an overview of our effectiveness and safety studies for the proposed indication ‘to control egg mortality associated with Saprolegniasis inf...

  8. Feeding Lactobacillus spp. and Bacillus spp. Does Not Improve Growth or Survival of Channel Catfish Experimentally Challenged with Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A major problem in the channel catfish industry has been high disease loss to enteric septicemia of catfish, caused by the bacterium Edwardsiella ictaluri. Feeding probiotics may prove beneficial in improving disease resistance. The first study examined the effects of a Lactobacillus probiotic (Flor...

  9. Identification and characterization of a FcR homolog in an ectothermic vertebrate, the channel catfish (Ictalurus punctatus)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A Fc receptor homolog (IpFcRI)3, representing the first such receptor from an ectothermic vertebrate, has been identified in the channel catfish (Ictalurus punctatus). Mining of the catfish expressed sequence tag (EST) databases using mammalian FcR sequences for CD16, CD32, and CD64 resulted in the ...

  10. Complete structure, genomic organization, and expression of channel catfish (Ictalurus punctatus, Rafinesque 1818) matrix metalloproteinase-9 gene

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In the course of studying pathogenesis of enteric septicemia of catfish, we noted that the channel catfish (CC) matrix metalloproteinase-9 (MMP-9) expressed sequence tag (EST) was up-regulated after early Edwardsiella ictaluri infection. In this study, the CC MMP-9 gene was cloned, sequenced and ch...

  11. Effects of Various Dietary Carotenoid Pigments on Fillet Appearance and Pigment Absorption in Channel Catfish Ictalarus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A study was conducted to evaluate effects of various carotenoids on skin and fillet coloration and fillet carotenoid concentration in channel catfish Ictalurus punctatus. For 12 weeks, juvenile catfish were fed one of six experimental diets containing no supplemental carotenoid or 100 mg/kg of one ...

  12. The effect of hydrogen peroxide on the hatch rate and Saprolegnia spp. infestation of channel catfish eggs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Infestations of fungi or water molds on channel catfish Ictalurus punctatus eggs can lower egg survival requiring the producer to spawn more catfish or risk fingerling shortages. Hydrogen peroxide treatments were evaluated to determine their effect on egg survival and efficacy against naturally occ...

  13. Effect of immunization of channel catfish with inactivated trophonts on serum and cutaneous antibody titers and survival against Ichthyophthirius multifiliis

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Two trials were conducted to determine the effect of immunization of channel catfish with inactivated trophonts on serum and cutaneous antibody titers and survival against Ichthyophthirius multifiliis Fouquet (Ich). In trial I, catfish were intraperitoneally (IP) immunized with: 1) 1% formalin-inac...

  14. Optimizing copper sulfate treatments for fungus control on channel catfish eggs in high alkalinity/moderate hardness water

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Interest in the use of copper sulfate (CuSO4) for fungus (Saprolegnia sp.) control in catfish hatcheries has developed over the past few years. A range-finding study was designed to determine the optimum concentration needed for fungus control on channel catfish eggs in 23.5°C well water at the Stu...

  15. Effects of chronic upper temperature regimes on growth of two geographic strains of channel and hybrid catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Climate change is a growing concern for pond culture of catfish, due to possible exacerbation of temperature fluctuations and increased maximum daily temperatures. Because channel catfish (Ictalurus punctatus) have a broad natural distribution from Canada to Mexico, it was hypothesized that natural ...

  16. Evaluation of potassium permanganate against an experimental subacute infection of Flavobacterium columnare in channel catfish, Icatlurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The efficacy of potassium permanganate (KMnO4) as a prophylactic and therapeutic treatment for subacute infection of Flavobacterium columnare was demonstrated in experimentally infected channel catfish, Ictalurus punctatus. Catfish experimentally infected with F. columnare to mimic a subacute infec...

  17. Increasing Fish Oil Levels in Commercial Diets Influence Hematology and Immune Responses of Channel Catfish, Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cultured freshwater fish including channel catfish are commonly fed grain-soybean meal based feeds high in linoleic series (n-6) fatty acids. Published studies have shown that supplementation of catfish diets with marine fish oil rich in n-3 highly unsaturated fatty acids (n-3 HUFA) significantly in...

  18. Histologic changes in channel catfish, Ictalurus punctatus Rafinesque, fed diets containing graded levels of gossypol-acetic acid

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A study was performed to evaluate the histologic changes among fingerling channel catfish (Ictalurus punctatus) fed purified diets containing graded levels of gossypol from gossypol–acetic acid. The catfish were maintained on diets with 0, 300, 600, 900, 1200 or 1500 mg gossypol kg-1 diet for 12 we...

  19. The effects of a sublethal dose of botulinum serotype E on the swimming performance of channel catfish fingerlings

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Visceral toxicosis of catfish (VTC) is a disease of cultured Channel Catfish Ictalurus punctatus in the Mississippi Delta region and surrounding states. The etiology of VTC is associated with botulinum serotype E (BoNT/E), which causes blockage of acetylcholine release at the neuromuscular junction,...

  20. Channel Catfish, Ictalurus punctatus, CD81 cDNA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    CD81, also known as the target of an antiproliferative antibody (TAPA) is a member of tetraspanin integral membrane protein family. This protein plays many important roles in immune functions. In this communication, we cloned, sequenced and characterized the channen catfish CD81 transcript. The comp...

  1. Antigens provide immunity against Ich in channel catfish trials

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Studies were conducted to determine effects of 1) types of Ich antigens and routes of immunization, 2) methods of inactivated trophonts, and 3) antigen doses on fish immune protection against Ichthyophthirius multifiliis Fouquet (Ich). All catfish immunized with live theronts by immersion, live the...

  2. A microsatellite-based genetic linkage map for channel catfish, Ictalurus punctatus.

    PubMed Central

    Waldbieser, G C; Bosworth, B G; Nonneman, D J; Wolters, W R

    2001-01-01

    Microsatellite loci were identified in channel catfish gene sequences or random clones from a small insert genomic DNA library. Outbred populations of channel catfish contained an average of eight alleles per locus and an average heterozygosity of 0.70. A genetic linkage map of the channel catfish genome (N = 29) was constructed from two reference families. A total of 293 microsatellite loci were polymorphic in one or both families, with an average of 171 informative meioses per locus. Nineteen type I loci, 243 type II loci, and one EST were placed in 32 multipoint linkage groups covering 1958 cM. Nine more type II loci were contained in three two-point linkage groups covering 24.5 cM. Twenty-two type II loci remained unlinked. Multipoint linkage groups ranged in size from 11.9 to 110.5 cM with an average intermarker distance of 8.7 cM. Seven microsatellite loci were closely linked with the sex-determining locus. The microsatellite loci and genetic linkage map will increase the efficiency of selective breeding programs for channel catfish. PMID:11404336

  3. Channel catfish CD8a and CD8ß co-receptors characterization expression and polymorphism

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In this study we report the identification and characterization of channel catfish, Ictalurus punctatus CD8a and CD8ß genes. Both genes encode predicted proteins containing a leader, a immunoglobulin superfamily V domain, a stalk/hinge region, a transmembrane region and a positively charged cytoplas...

  4. Threadfin shad impacts phytoplankton and zooplankton community structures in channel catfish ponds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Plankton community structure and chlorophyll a concentration were compared in 12 0.1-ha earthen ponds co-stocked with channel catfish (Ictalurus punctatus Rafinesque, 1818) in multiple-batch culture (initial biomass = 5,458 kg ha-1) and a planktivore, threadfin shad (Dorosoma petenense Güther, 1867;...

  5. Sex Genotype and Sex Phenotype Contribute to Growth Differences Between Male and Female Channel Catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish have an XX:XY genotypic system of sex determination, and until the present study, the influence of sex genotype on growth could not be distinguished from sex phenotype. Genotypic male fish (XY) were produced by mating normal (XX) female fish with YY male fish. A subsample from eac...

  6. Molecular cloning and expression of channel catfish, Ictalurus punctatus, complement membrane attack complex inhibitor CD59

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The channel catfish, Ictalurus punctatus, complement membrane attack complex inhibitor CD59 gene was cloned and analyzed. Total RNA from tissues was isolated and cDNA libraries were constructed by the rapid amplification cDNA end (RACE) method. The gene-specific primers in conjunction with the RAC...

  7. EVALUATION OF PROTEIN REDUCTION AND LYSINE SUPPLEMENTATION OF PRODUCTION DIETS FOR CHANNEL CATFISH ICTALURUS PUNCTATUS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A 2-year continuous production trial was conducted in earthen ponds to evaluate lysine supplementation of practical diets as a means to reduce the need for total dietary protein and limit nitrogenous waste production of channel catfish. Experimental diets consisted of three practical diets containi...

  8. Global transcription analysis of vaccinated channel catfish following challenge with virulent Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    To determine the identities of genes involved in either innate or adaptive immunity, microarray analysis of 65,182 UniGene transcripts were performed to compare gene expression in vaccinated channel catfish after challenge with a virulent Edwardsiella ictaluri compared to that in sham-vaccinated fis...

  9. Flavobacterium columnare genomovar type influenced mortality in channel catfish Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Genetic diversity has been documented and confirmed in Flavobacterium columnare, causal agent of columnaris disease. This aquatic bacterium is pathogenic to many species of freshwater fish throughout the world, and the United States channel catfish (Ictalurus punctatus) aquaculture industry is seve...

  10. Tetraspanin 3 and tetraspanin 7 transcripts of channel catfish, Ictalurus punctatus Rafinesque, 1818

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Tetraspanin 3 and tetraspanin 7 proteins are members of the tetraspanin integral membrane protein family. These proteins play many important roles in immuno-physiological functions. In this communication, we sequenced and characterized the channel catfish tetraspanin 3 and tetraspanin 7 transcript...

  11. Peracetic acid is effective for controlling fungus on channel catfish eggs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Peracetic acid (PAA) is a relatively new compound suggested for use to treat pathogens in aquaculture. It is approved for use in Europe, but not in the United States. This study determined the effectiveness of PAA for fungus control on channel catfish Ictalurus punctatus eggs. The study consisted...

  12. A Novel Henneguya Species from Channel Catfish Described by Morphological, Histological, and Molecular Characterization

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish Ictalurus punctatus from a commercial farming operation in the Mississippi Delta were submitted for examination for the presence of infection by the trematode Bolbophorus damnificus. The fish were instead found to possess skin nodules suggestive of Henneguya pellis, a species previou...

  13. Optimizing CuSO4 treatments for fungus control on channel catfish eggs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This range-finding study determined the optimum concentration of copper sulfate (CuSO4) for fungus (Saprolegnia sp.) control on channel catfish Ictalurus punctatus eggs. The study consisted of five CuSO4 concentrations (2.5, 5, 10, 20 and 40 mg/L) and an untreated control. A single spawn was used ...

  14. Production Responses of Channel Catfish to Minimum Daily Dissolved Oxygen Concentrations in Earthen Ponds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The purpose of this study was to determine the effects of the minimum daily dissolved oxygen (DO) concentration on production parameters of channel catfish Ictalurus punctatus in earthen ponds. Fifteen one-acre ponds (5 ponds per treatment) were managed as High Oxygen (minimum DO concentrations aver...

  15. Use of corn germ meal in diets for pond-raised channel catfish, Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Corn germ meal (CGM) is a by-product of corn milling. On the basis of its nutrient composition and digestibility values, it appears to be a suitable ingredient for use in channel catfish, Ictalurus punctatus, diets. A study was conducted to examine the use of various levels of CGM in diets for pond-...

  16. Channel catfish, Ictalurus punctatus, chemokine receptor CXCR4 cDNA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Chemokine receptor CXCR4, a member of the G protein-coupled receptor superfamily, binds selectively CXCL12. This protein plays many important roles in immunological as well as pathophysiological functions. In this communication, we identified and characterized the channel catfish CXCR4 transcript....

  17. Pre and postprandial changes in orexigenic and anorexigenic factors in channel catfish Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We examined pre- and postprandial changes in the expression of plasma ghrelin (GHRL) and mRNAs encoding GRLN, cocaine and amphetamine regulated transcript (CART), neuropeptide Y (NPY), and cholecystokinin (CCK) in channel catfish. Fish were either offered feed (Fed) or fasted (Unfed). Feeding incr...

  18. Evaluation of Various Feeding Regimens in A Multiple-Batch Cropping System of Channel Catfish Production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A four-year pond study was conducted to compare gross production, feed conversion, processing yield, and body composition of channel catfish Ictalurus punctatus fed once daily or every other day to satiation, or # 110 kg/ha per day in a multiple-batch cropping system. The greatest amount of feed fed...

  19. Effects of Dietary Protein Concentration and Feeding Regimen on Channel Catfish Ictalurus punctatus Production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A factorial experiment was conducted to examine effects of dietary protein concentration (24, 28, 32, or 36%) and feeding regimen (feeding once daily or every other d) on channel catfish Ictalurus punctatus production in earthen ponds. Compared with fish fed daily, fish fed every other d had lower ...

  20. Maternal Transfer of Vitamin C in Channel Catfish (Ictalurus Punctatus) Effects Reproduction and Progeny Performance

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Two routes of maternal transfer of vitamin C in channel catfish female broodfish prior to spawning were explored as a strategy to incorporate the vitamin to determine its effect on reproduction and subsequent performance of the progeny. Accumulation of vitamin C was higher (p<0.05) in ovarian tissu...

  1. Mitochondrial fuction and growth in channel catfish strain and diet effects

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A 2 x 6 factorial experiment was conducted to evaluate the effects of commercial diets (32/4 or 36/6 percent protein/fat) and channel catfish strains (A, B, C, D, E, or F) on the growth performance, mitochondrial respiratory chain enzyme activities and gene expression levels in the liver, muscle, an...

  2. The Case Against Using Organic Fertilizers in Channel Catfish Ictalurus punctatus Nursery Ponds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This paper reviews the assumed advantages and disadvantages of organic fertilizers and presents the case that the risks outweigh the benefits for channel catfish, Ictalurus punctatus, nursery pond fertilization. Under certain conditions, organic fertilizers may be beneficial to provide forage for z...

  3. Response of the Somatotropic Axis to Alterations in Feed Intake of Channel Catfish (Ictalurus punctatus)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    To better understand the effects of reduced feeding frequency on the GH-IGF-I axis, channel catfish, (Ictalurus punctatus), were either fed (Fed control, commercial diet fed daily), fed every other day (FEOD, commercial diet fed every other day), or not fed (Unfed, no feed). Pituitary GH mRNA incre...

  4. MOLECULAR CLONING AND EXPRESSION OF CHANNEL CATFISH, ICTALURUS PUNCTATUS, COMPLEMENT MEMBRANE ATTACK COMPLEX INHIBITOR CD59

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The channel catfish, Ictalurus punctatus, complement membrane attack complex inhibitor CD59 gene was cloned and analyzed. Total RNA from tissues was isolated and cDNA libraries were constructed by the rapid amplification cDNA end (RACE) method. The gene-specific primers in conjunction with the RAC...

  5. Winter performance of an outdoor biofloc production system for channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In the tropics, outdoor biofloc technology production systems are operated year-round. While channel catfish (Ictalurus punctatus) have been grown successfully in an outdoor biofloc production system, these studies were conducted only during the growing season and production tanks were idled for the...

  6. Identification of SHIP-1 and SHIP-2 homologs in channel catfish, Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Src homology domain 2 (SH2) domain-containing inositol 5’-phosphatases (SHIP) proteins have diverse roles in signal transduction. SHIP-1 and SHIP-2 homologs were identified in channel catfish, Ictalurus punctatus, based on sequence homology to murine and human SHIP sequences. Full-length cDNAs for ...

  7. Age at Puberty of Channel Catfish, Ictalurus punctatus, Controlled by Thermoperiod

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish held under a shortened annual temperature cycle of four months of warm water followed by two months of cold temperature spawned at a higher rate (73%) after three temperature cycles than fish held in outdoor ponds on two normal seasonal temperature cycles (10%) and fish raised in tan...

  8. Expression profiles of seven channel catfish antimicrobial peptides in response to Edwardsiella ictaluri infection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Using quantitative PCR technique, the relative transcriptional levels of seven channel catfish antimicrobial peptide (AMP) genes [NK-lysin type 1, NK-lysin type 2, NK-lysin type 3, bactericidal permeability-increasing protein (BPI), cathepsin D, hepcidin, and liver-expressed antimicrobial peptide 2 ...

  9. Pre-treating channel catfish with copper sulfate affects susceptibility to columnaris disease

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Columnaris disease is one of the most important bacterial diseases of channel catfish, Ictalurus punctatus, commercially grown in the US. This disease can greatly diminish the profitability of aquaculture operations by large-scale mortality events, particularly in the fingerling production phase. ...

  10. Channel catfish (Ictalurus punctatus) CD156a mRNA sequence

    Technology Transfer Automated Retrieval System (TEKTRAN)

    CD156a, also known as A Disintegrin And Metalloproteinase domain 8 (ADAM8), is a member of ADAM family. We cloned, and sequenced the channel catfish CD156a transcript. The full-length of the CD156a cDNA consisted of 3035 nucleotides, including a 5’-untranslated region (UTR), an open reading frame ...

  11. Characterization of additional novel immune type receptors in channel catfish, Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Mining of channel catfish (Ictalurus punctatus) expressed sequence tag databases identified seven new novel immune type receptors (IpNITRs). These differed in sequence, but not structure, from previously described IpNITR1-11. IpNITR12a, 12b, 13 and 14, encode proteins containing a single variable (V...

  12. Apparent digestibility of alternative plant-protein feedstuffs for channel catfish, Ictalurus punctatus (Rafinesque)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A study was conducted with channel catfish, Ictalurus puntatus to determine apparent digestibility/availability coefficients of protein, amino acids, lipid and energy for alternative plant-protein feedstuffs: corn gluten feed, corn germ meal, distillers dried grains with solubles, and canola meal, c...

  13. Production of homozygous, doubled haploid channel catfish via hydrostatic pressure and thermal treatments

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Production of doubled haploids via mitotic gynogenesis is a useful tool for the creation of completely inbred fish. In order to produce viable doubled haploid channel catfish, we utilized hydrostatic pressure or thermal treatments on eggs fertilized with sperm that had been exposed to ultraviolet l...

  14. Septin genes in channel catfish (Ictalurus punctatus) and their involvement in disease defense responses.

    PubMed

    Fu, Qiang; Li, Yun; Yang, Yujia; Li, Chao; Yao, Jun; Zeng, Qifan; Qin, Zhenkui; Liu, Shikai; Li, Daoji; Liu, Zhanjiang

    2016-02-01

    Septins are an evolutionarily conserved family of GTP-binding proteins. They are involved in diverse processes including cytokinesis, apoptosis, infection, neurodegeneration and neoplasia. In this study, through thorough data mining of existed channel catfish genomic resources, we identified a complete set of 15 septin genes. Septins were classified into four subgroups according to phylogenetic analysis. Extensive comparative genomic analysis, including domain and syntenic analysis, supported their annotation and orthologies. The expression patterns of septins in channel catfish were examined in healthy tissues and after infection with two major bacterial pathogens, Edwardsiella ictaluri and Flavobacterium columnare. In healthy channel catfish, most septin genes were ubiquitously expressed and presented diversity patterns in various tissues, especially mucosal tissues, proposing the significant roles septin genes may play in maintaining homeostasis and host immune response activities. After bacterial infections, most septin genes were regulated, but opposite direction in expression profiles were found with the two bacterial pathogens: the differentially expressed septin genes were down-regulated in the intestine after E. ictaluri infection while generally up-regulated in the gill after F. columnare infection, suggesting a pathogen-specific and tissue-specific pattern of regulation. Taken together, these results suggested that septin genes may play complex and important roles in the host immune responses to bacterial pathogens in channel catfish. PMID:26700173

  15. Stress Effects in Channel Catfish (Ictalurus punctatus) Fry on Pond Survival

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Results from previous studies suggested that channel catfish (Ictalurus punctatus) fry that were housed in very high traffic areas from hatch until reaching 9 g in size were able to survive experimental challenge with Edwardsiella ictaluri better than siblings who were hatched and raised in very low...

  16. Influences of cyclic, high temperatures on juvenile channel catfish growth and feeding

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Effects of high summer temperatures on channel catfish (Ictalurus punctatus) are poorly understood, particularly for thermal regimes that mimic pond aquaculture conditions. Therefore, this study examined the effects of three cycling upper-range temperature regimes (23-27ºC, 27-31ºC, and 31-35ºC) cha...

  17. Effects of fry age-at-stocking on growth and survival of channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Large numbers of fry are required to supplystocking needs in the channel catfish, Ictaluruspunctatus, industry. In January 2009, fingerlings held on farms totaled 728 million (NASS 2009). Survival of fry after stocking into ponds in not well documented, and reported values range from about 55% (Hatc...

  18. Acute toxicity and histopathology of channel catfish fry exposed to peracetic acid

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish Ictalurus punctatus yolk-sac fry and swim-up fry were exposed to peracetic acid (PAA) for 48h in static toxicity bioassays at 23C. The test water was 217 and 126 mg/L (as CaCO3) total alkalinity and total hardness, respectively. Probit LC50 values were estimated with the trimmed Sp...

  19. Treating a natural outbreak of columnaris in channel catfish with copper sulfate and potassium permanganate

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An F. Columnare-exclusive epizootic occurred in fingerling channel catfish (Ictalurus punctatus) during normal tank culture practices at SNARC. Fish were transferred to the ultra low-flow system and 2.1 mg/L copper sulfate or 3 mg/L potassium permanganate was administered; an untreated control was ...

  20. Effect of chronic, nightly hypoxia on growth and yield of channel x blue hybrid catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Performance traits and metabolic responses of the channel x blue hybrid catfish (Ictalurus punctatus female, Jubilee strain x I. furcatus male, D&B strain) in response to chronic nightly hypoxia were evaluated in this 197-d study. Sixteen 0.25-acre earthen ponds were stocked with 6,141 hybrid catfis...

  1. The channel catfish genome sequenc provides insights into the evolution of scale formation in teleosts

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish (Ictalurus punctatus), characteristic of its scaleless skin and prominent barbels, is an ideal species to study osteogenesis, development of appendages, olfactory sensing, and immunological adaptation. It is the leading aquaculture species in the United States. A high quality refere...

  2. Effects of mannanoligosaccharides on growth and immune function in channel catfish challenged with Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The high temperature and pressure achieved during cooking extrusion has been shown to affect nutrient availability. To determine the effects of extrusion temperature on the efficacy of mannanoligosaccharide (Bio-Mos) in channel catfish, 2 commercial and 4 experimental diets were fed for 9 wks and t...

  3. INDUCTION OF NITRIC OXIDE IN CHANNEL CATFISH ICALUTUS PUNCTATUS BY EDWARDSIELLA ICTALURI

    EPA Science Inventory

    Channel catfish, Ictalurus punctatus, were injected intraperiloneally with live cells of Edwardsiella ictaluri and held in flow-through tanks for five days. he head kidneys from injected and control fish were removed and the nitric oxide synthase activity measured. ctivities foun...

  4. Channel catfish (Ictalurus punctatus Rafinesque, 1818) annexin A1 and A6 transcripts

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Annexins, a protein superfamily, are critical in many important immuno-physiological processes. In this communication, we sequenced and characterized channel catfish orthologs to human annexin A1 and A6. Total RNA from tissues was extracted and cDNA libraries were constructed by the rapid amplifica...

  5. DIETARY LIPID AS A FACTOR MODULATING XENOBIOTIC METABOLISM IN CHANNEL CATFISH (ICTALURUS PUNCTATUS)

    EPA Science Inventory

    Adult channel catfish (Ictaiurus punctatus) were fed diets containing menhaden oil (MHO), soybean oil (SBO), or beef tallow (BFT) as lipid sources for 116 d. The effects of these diets on two important hepatic xenobiotic-metabolizing enzyme systems, cytochrome p-45O-dependent mon...

  6. Effects of Fasting on IGF-Binding Proteins, Glucose, and Cortisol in Channel Catfish (Ictalurus punctatus)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The effects of fasting on IGF-binding proteins, glucose, and cortisol in channel catfish were examined. Fed fish (controls) were compared to 14-, 30-, and 45-day fasted fish and 45-day fasted fish refed for 15 additional days. Body length and body weight changes, condition factor(CF), hepatosomati...

  7. Experimental evaluation of the effect of winter feeding on channel catfish growout pond plankton

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Ten, 0.25 acre ponds at the UAPB Aquaculture Station were sampled weekly from Dec. 7-Feb. 22 (n=90) for phytoplankton and zooplankton. Five of the ponds were randomly assigned to each of two treatments: no feeding and feeding based on recommended rates. Channel catfish sizes and numbers approximated...

  8. Single intravenous and oral dose pharmacokinetics of florfenicol in the channel catfish Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Plasma distribution and elimination of florfenicol in channel catfish were investigated after a single dose (10mg/kg) of intravenous i.v.) or oral administration in freshwater at a mean water temperature of 25.4°C. Florfenicol concentrations in plasma were analyzed by means of liquid chromatography...

  9. Determination of the median lethal dose of botulinum serotype E in channel catfish fingerlings

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The median lethal dose of botulinum serotype E in 5.3-g channel catfish Ictalurus punctatus fingerlings was determined. Five tanks (five fish/tank) were assigned to each of the following treatment groups: 70, 50, 35, 25, or 15 pg of purified botulinum serotype E. Fish were injected intracoelomically...

  10. PHYSIOLOGICALLY-BASED TOXICOKINETIC MODELING OF THREE WATERBORNE CHLOROETHANES IN CHANNEL CATFISH, ICTALURUS PUNCTATUS

    EPA Science Inventory

    A physiologically-based toxicokinetic model for fish was used to describe the uptake and disposition of three chlorinated ethanes in channel catfish (Ictalurus punctatus). atfish were simultaneously exposed to 1,1,2,2-tetrachloroethane (TCE), pentachloroethane (PCE), and hexachlo...

  11. RESPIRATORY-CARDIOVASCULAR PHYSIOLOGY AND CHLOROETHANE GILL FLUX IN THE CHANNEL CATFISH ICTALRUS PUNCTATUS

    EPA Science Inventory

    A fish respirometer-metabolism chamber was used to obtain in ivo respiratory-cardiovascular and chloroethane gill flux data on transected channel catfish (Ictalrus punctatus). ethods used for spinal transection, attachment of an oral membrane (respiratory mask), placement and att...

  12. Parasitism by protozoan Ichthyophthirius multifiliis enhanced invasion of Aeromonas hydrophila in tissues of channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Ichthyophthirius multifiliis Fouquet (Ich) and Aeromonas hydrophila are two common pathogens of cultured fish. Currently there is no information available for the effect of coinfection by Ich and A. hydrophila on bacterial load and survival in channel catfish. Two trials were conducted in this stud...

  13. Complete genome sequence of Edwardsiella tarda (isolate FL95-01)recovered from channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Edwardsiella tarda is a Gram-negative facultative anaerobe isolated from fish, reptiles, amphibians, and mammals, including humans. This is a report of the complete and annotated genome of E. tarda isolate FL95-01, recovered from channel catfish (Ictalurus punctatus)....

  14. Dietary Bovine Lactoferrin Increases Resistance of Juvenile Channel Catfish (Ictalurus punctatus) to Enteric Septicemia

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Juvenile channel catfish (Ictalurus punctatus) were fed nutritionally complete, practical basal diets supplemented with bovine lactoferrin (Lf) at 0, 200, 400, 800, or 1600 mg/kg diet to apparent satiation twice daily for 5 weeks. Feed intake was significantly higher in fish fed diets supplemented w...

  15. Molecular Cloning and Sequencing of Channel Catfish, Ictalurus punctatus, Cathepsin H and L cDNA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cathepsin H and L, a lysosomal cysteine endopeptidase of the papain family, are ubiquitously expressed and involve in antigen processing. In this communication, the channel catfish cathepsin H and L transcripts were sequenced and analyzed. Total RNA from tissues was extracted and cDNA libraries we...

  16. Effects of dietary copper supplements on the resistance of channel catfish to columnaris disease

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper is an essential dietary component in animal feeds. Copper sulfate has also been shown to be therapeutic and prophylactic as a water treatment for several indications including columnaris disease. Columnaris disease is one of the most important bacterial diseases of channel catfish commercial...

  17. Dietary copper effects survival of channel catfish challenged with Flavobacterium columnare

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Columnaris disease is one of the most important bacterial diseases of channel catfish, Ictalurus punctatus, commercially grown in the US. Copper sulfate (CuSO4) has also been shown to be both therapeutic and prophylactic as a water treatment for columnaris disease. As copper is an essential dietar...

  18. Rhamnose binding lectins influence Flavobacterium columnare pathogenesis in channel catfish Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Columnaris disease, caused by the bacterial pathogen Flavobacterium columnare, continues to be a major problem worldwide and commonly leads to tremendous losses of both wild and cultured freshwater fish, particularly in intensively farmed aquaculture species such as channel catfish. Despite its ecol...

  19. HYDROGEN PEROXIDE TREATMENT DURING EGG INCUBATION IMPROVES CHANNEL CATFISH HATCHING SUCCESS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Three trials were conducted to evaluate the effect of hydrogen peroxide (H2O2) treatment on channel catfish Ictalurus punctatus hatching success when administered during egg incubation as a 15 min. bath or as a flow-through treatment. In the first trial, initial treatment with 100 ppm povidone iodi...

  20. Multidose pharmacokineetecs of orally administered florfenicol in channel catfish Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Plasma disposition of florfenicol in channel catfish was investigated after an oral dose (10mg/kg for 10 days) administered in freshwater at water temperatures ranging from 24.7 to 25.9°C. Florfenicol concentrations in plasma were analyzed by means of liquid chromatography with MS/MS detection. Af...

  1. DEET (N,N-diethyl-m-toluamide) toxicity to channel catfish Ictalurus punctatus sac fry

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The combination of open facilities, moisture, and warm weather during channel catfish Ictalurus punctatus spawning season causes mosquito-infestation problems. A common solution to mosquito problems in hatcheries is to use mosquito repellents applied to exposed skin and clothing. DEET (N,N-diethyl-...

  2. Sequencing of channel catfish, Ictalurus punctatus, cyclophilin A cDNA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cyclophilin A is a member of highly conserved immunophilins and ubiquitously found intracellularly. The complete sequence of the channel catfish cyclophilin A cDNA gene consisted of 1,170 nucleotides. Analysis of the nucleotide sequence reveals one open reading frame and 5’- and 3’-end untranslate...

  3. Plasma cortisol stress response in channel catfish, Ictalurus punctatus influences susceptibility to Edwardeseilla ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cortisol is a primary stress hormone in fish as its plasma variations correlate with the occurrence of various stressful situations. Past studies have demonstrated that fish subjected to handling stress or poor water quality had a reduced ability to resist pathogens. Channel catfish fingerlings th...

  4. NUTRITIONAL VALUE OF HEAT-TREATED SOYBEAN MEAL FOR CHANNEL CATFISH (ICTALURUS PUNCTATUS)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A study was undertaken to evaluate the effect of heat treatment of defatted raw soybean meal (RSBM) on the growth, hematology, immune response and resistance of channel catfish to Edwardsiella ictaluri. Six isocaloric and isonitrogenous practical diets were formulated. A diet containing 45% of comm...

  5. Clearance of yellow pigments lutein and zeathanxin in channel catfish reared at different water temperatures

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A study was conducted to determine clearance time of yellow pigments lutein and zeaxanthin in channel catfish at various temperatures. Fish of initial weight of 13.4 g were stocked into flow-through aquaria and fed once daily with a yellow pigment enhanced diet for 11 weeks when the yellow color be...

  6. Channel Catfish, Ictalurus punctatus, ubiquitin carboxy-terminal hydrolase L5 cDNA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The ubiquitin-proteasome cycle is a complex, non-lysosomal biochemical process for intracellular protein degradation. This process involves many enzymes. One of them is ubiquitin carboxy-terminal hydrolase (UCT). In this report, we cloned, sequenced and characterized the channel catfish UCT L5 cDNA....

  7. Effectiveness of copper sulfate and potassium permanganate on channel catfish infected with Flavobacterium columnare

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper sulfate (CuSO4) and potassium permanganate (KMnO4) were evaluated for their effectiveness to curtail mortality and decrease bacterial load in fish tissues and water in channel catfish Ictalurus punctatus naturally infected with Flavobacterium columnare, the causative agent of columnaris. Fis...

  8. Evaluation of Glycerol from Biodiesel Production as a Feed Ingredient for Channel Catfish, Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Glycerol is the main by-product of biodiesel production from vegetable oils and animal fats. It has been evaluated as an energy source for several farm animals. A study was conducted to examine the effects of various levels of glycerol in channel catfish, Ictalurus punctatus, diets. Fish with mean i...

  9. Effects of hatching conditions on triple tail deformity in channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish (Ictalurus punctatus) occasionally will be found with abnormal caudal fin structures. These deformities vary in size and appearance but generally occur as an extra fin protruding from the lateral surface of the caudal peduncle. The phrase “triple-tail” is often used to describe fis...

  10. Streptococcal Arthritis, Osteolysis, Myositis, and Spinal Meningitis in Channel Catfish Ictalurus punctatus Broodstock

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This report details findings of an investigation into complaints by commercial fingerling producers of low-grade mortalities, poor reproductive success, emaciation, skin lesions, and severely arched backs among broodstock of channel catfish Ictalurus punctatus. Gross lesions involved the jaw, fin ba...

  11. Recent Improvements in the Channel Catfish, Ictalurus punctatus Female X Blue Catfish, I. furcatus Male Hybrid Catfish Production: Hatchery Spawning Replace Natural Spawning in the Catfish Industry?

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The catfish industry has stagnated during the last 7 years and production has actually decreased by a third in an industry that was once doubling in size every 10 years. The industry is currently struggling to keep pace with the increasing cost of feed, high cost of fuel, production inefficiencies ...

  12. Sublethal copper stress and susceptibility of channel catfish to experimental infections with Ichthyophthirius multifiliis

    SciTech Connect

    Ewing, M.S.; Ewing, S.A.; Zimmer, M.A.

    1982-06-01

    The study describes a technique for evaluating the change in susceptibility of channel catfish (Ictalurus punctatus) to infection by the protozoan parasite Ichthyophthirius multifiliis following exposure to sublethal concentrations of dissolved copper. This ubiquitous parasite infects a wide variety of freshwater fishes and causes severe disease problems in catfish. Exposure to sublethal concentrations of contaminants may amplify stress significantly for fish exposed to such disease agents. Therefore, techniques are needed to judge the effect of contaminant stress upon the course of disease, rather than simply death from disease, in fishes. Results show that pathologic changes attendent upon exposure to the parasite and those upon exposure to copper are complementary in effect. (JMT)

  13. Identification and characterization of TCRγ and TCRδ chains in channel catfish, Ictalurus punctatus.

    PubMed

    Moulana, Mohadetheh; Taylor, Erin B; Edholm, Eva-Stina; Quiniou, Sylvie M A; Wilson, Melanie; Bengtén, Eva

    2014-10-01

    Channel catfish, Ictalurus punctatus, T cell receptors (TCR) γ and δ were identified by mining of expressed sequence tag databases, and full-length sequences were obtained by 5'-RACE and RT-PCR protocols. cDNAs for each of these TCR chains encode typical variable (V), diversity (D), joining (J), and constant (C) regions. Three TCRγ V families, seven TCRγ J sequences, and three TCRγ C sequences were identified from sequencing of cDNA. Primer walking on bacterial artificial chromosomes (BACs) confirmed that the TRG locus contained seven TRGJ segments and indicated that the locus consists of (Vγ3-Jγ6-Cγ2)-(Vγ1n-Jγ7-Cγ3)-(Vγ2-Jγ5-Jγ4-Jγ3-Jγ2-Jγ1-Cγ1). In comparison for TCRδ, two V families, four TCRδ D sequences, one TCRδ J sequence, and one TCRδ C sequence were identified by cDNA sequencing. Importantly, the finding that some catfish TCRδ cDNAs contain TCR Vα-D-Jδ rearrangements and some TCRα cDNAs contain Vδ-Jα rearrangements strongly implies that the catfish TRA and TRD loci are linked. Finally, primer walking on BACs and Southern blotting suggest that catfish have four TRDD gene segments and a single TRDJ and TRDC gene. As in most vertebrates, all three reading frames of each of the catfish TRDD segments can be used in functional rearrangements, and more than one TRDD segment can be used in a single rearrangement. As expected, catfish TCRδ CDR3 regions are longer and more diverse than TCRγ CDR3 regions, and as a group they utilize more nucleotide additions and contain more nucleotide deletions than catfish TCRγ rearrangements. PMID:25129471

  14. DNA damage and radiocesium in channel catfish from Chernobyl

    SciTech Connect

    Sugg, D.W.; Brooks, J.A.; Jagoe, C.H.; Smith, M.H.; Chesser, R.K.; Bickham, J.W.; Lomakin, M.D.; Dallas, C.E.; Baker, R.J.

    1996-07-01

    The explosion of the Chernobyl Nuclear Power Plant resulted in some of the most radioactively contaminated habitats on earth. Despite evacuation of all human inhabitants from the most contaminated areas, animals and plants continue to thrive in these areas. This study examines the levels of contamination and genetic damage associated with cesium-137 in catfish (Ictalurus punctatus) from the cooling pond and a control site. In general, catfish from the cooling pond exhibit greater genetic damage, and the amount of damage is related to the concentration of radiocesium in individual fish. Genetic damage is primarily in the form of DNA strand breaks, with few micronuclei being observed in contaminated fish. The possible roles that acclimation and adaption play in the response to high levels of radiation exposure are discussed.

  15. Plant Natural Compounds with Antibacterial Activity Towards Common Pathogens of Pond-Cultured Channel Catfish (Ictalurus punctatus)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The bacteria Edwardsiella ictaluri and Flavobacterium columnare cause enteric septicemia and columnaris disease, respectively, in channel catfish (Ictalurus punctatus). Natural therapeutants may provide an alternative to current management approaches used by producers. In this study, a rapid bioas...

  16. Evaluation of a cohabitation challenge model in immunization trials for channel catfish, Ictalurus punctatus (Rafinesque), against Ichthyophthirius multifiliis

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Calcein marking and cohabitation challenge have not been investigated in fish parasite research. This study evaluated a cohabitation challenge method in immunization trials against Ichthyophthirius multifiliis (Ich) using calcein, a fluorescent dye, to mark channel catfish Ictalurus punctatus (Rafi...

  17. Effect of stocking biomass on solids, phytoplankton communities, common off-flavors, and production parameters in a channel catfish biofloc technology production system

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The effect of initial channel catfish (Ictalurus punctatus, Rafinesque, 1818) fingerling biomass (1.4, 1.8, or 2.3 kg m-3) on phytoplankton communities, common off-flavors, and stocker catfish production parameters was evaluated in biofloc technology production tanks. Stocker catfish size (145.5 – 1...

  18. G-protein coupled receptor 18 (GPR18) in channel catfish: Expression analysis and efficacy as immunostimulant against Aeromonas hydrophila infection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The objectives of this study were: 1) to determine the transcriptional profiles of G-protein coupled receptor 18 (GPR18) in channel catfish after infection with A. hydrophila compared to that in healthy catfish; 2) to determine whether over-expression of GPR18 in catfish gill cells will offer protec...

  19. Phylogenetic sequence analysis, recombinant expression, and tissue distribution of a channel catfish estrogen receptor beta

    USGS Publications Warehouse

    Xia, Zhenfang; Gale, William L.; Chang, Xiaotian; Langenau, David; Patino, Reynaldo; Maule, Alec G.; Densmore, Llewellyn D.

    2000-01-01

    An estrogen receptor β (ERβ) cDNA fragment was amplified by RT-PCR of total RNAextracted from liver and ovary of immature channel catfish. This cDNA fragment was used to screen an ovarian cDNA library made from an immature female fish. A clone was obtained that contained an open reading frame encoding a 575-amino-acid protein with a deduced molecular weight of 63.9 kDa. Maximum parsimony and Neighbor Joining analyses were used to generate a phylogenetic classification of channel catfish ERβ on the basis of 25 full-length teleost and tetrapod ER sequences. The consensus tree obtained indicated the existence of two major vertebrate ER subtypes, α and β. Within each subtype, and in accordance with established phylogenetic relationships, teleost and tetrapod ER were monophyletic confirming the results of a previous analysis (Z. Xiaet al., 1999, Gen. Comp. Endocrinol. 113, 360–368). Extracts of COS-7 cells transfectedwith channel catfish ERβ cDNA bound estrogen with high affinity (Kd = 0.21 nM) and specificity. The affinity of channel catfish ERβ for estrogen was higher than previously reported for channel catfish ERα. As determined by qualitative RT-PCR, the tissue distributions of ERα and ERβ were similar but not identical. Both ER subtypes were present in ovary and testis. ERα was found in all other tissues examined from juvenile and mature fish of both sexes. ERβ was also found in most tissues except, in most cases, whole blood and head kidney. Interestingly, the pattern of expression of ER subtypes in head kidney always corresponded to the pattern in whole blood. In conclusion, we isolated a channel catfish ERβ with ligand-binding affinity and tissue expression patterns different from ERα. Also, we confirmed the validity of our previously proposed general classification scheme for vertebrate ER into α and β subtypes and within each subtype, into teleost and tetrapod clades.

  20. Efficacy of oxytetracycline hydrochloride bath immersion to control external columnaris disease on walleye and channel catfish fingerlings

    USGS Publications Warehouse

    Rach, J.J.; Johnson, Aaron H.; Rudacille, J.B.; Schleis, S.M.

    2008-01-01

    The efficacy of oxytetracycline hydrochloride (OTC-HCl) in controlling external columnaris disease caused by Flavobacterium columnare on fingerling walleyes Sander vitreus and channel catfish Ictalurus punctatus was evaluated in two on-site hatchery trials. Microscopic examination of skin scrapings before treatment confirmed the presence of bacteria with characteristics indicative of F. columnare.in separate trials, walleyes (4.4 g) and channel catfish (1.5 g) were exposed to 60-min static bath treatments of OTC-HCl at 0, 10, and 20 mg/L (walleyes) or 0, 10, 20, and 40 mg/L (channel catfish) on three consecutive days. Each treatment regimen was tested in triplicate, and each replicate contained either 30 walleyes or 55 channel catfish. Posttreatment presumptive disease diagnosis indicated that F. columnare was the disease agent causing the mortality in both species of fish. Walleye survival at 10 d posttreatment was greater in the 10- and 20-mg/L treatment groups than in the control group; however, only the 10-mg/L treatment significantly (P < 0.05) increased walleye survival in comparison with controls. In the channel catfish trial, survival at 10 d posttreatment was significantly (P < 0.05) greater for all OTC-HCl treatment groups relative to controls. Results from these trials indicated that OTC-HCl treatments effectively reduced mortality in walleyes (10 mg/L only) and channel catfish infected with F. columnare. ?? Copyright by the American Fisheries Society 2008.

  1. Catch of channel catfish with tandem-set hoop nets and gill nets in lentic systems of Nebraska

    USGS Publications Warehouse

    Richters, Lindsey K.; Pope, Kevin L.

    2011-01-01

    Twenty-six Nebraska water bodies representing two ecosystem types (small standing waters and large standing waters) were surveyed during 2008 and 2009 with tandem-set hoop nets and experimental gill nets to determine if similar trends existed in catch rates and size structures of channel catfish Ictalurus punctatus captured with these gears. Gear efficiency was assessed as the number of sets (nets) that would be required to capture 100 channel catfish given observed catch per unit effort (CPUE). Efficiency of gill nets was not correlated with efficiency of hoop nets for capturing channel catfish. Small sample sizes prohibited estimation of proportional size distributions in most surveys; in the four surveys for which sample size was sufficient to quantify length-frequency distributions of captured channel catfish, distributions differed between gears. The CPUE of channel catfish did not differ between small and large water bodies for either gear. While catch rates of hoop nets were lower than rates recorded in previous studies, this gear was more efficient than gill nets at capturing channel catfish. However, comparisons of size structure between gears may be problematic.

  2. Dermal absorption of three waterborne chloroethanes in rainbow trout (Oncorhynchus mykiss) and channel catfish (Ictalurus punctatus).

    PubMed

    McKim, J M; Nichols, J W; Lien, G J; Hoffman, A D; Gallinat, C A; Stokes, G N

    1996-06-01

    In vivo estimates of xenobiotic chemical flux across the dermal surface of intact fish were obtained by measuring chemical loss from venous blood to expired water. An experimental system was developed to separate the dermal route of exposure from all other routes. The system was then used to measure dermal absorption of tetrachloroethane (TCE), pentachloroethane (PCE), and hexachloroethane (HCE) in channel catfish (Ictalurus punctatus) and rainbow trout (Oncorhynchus mykiss), two fish with very different skin anatomies. The kinetics of accumulation varied among chemicals, but for each compound were similar among species. TCE accumulated rapidly, reaching steady state in blood within 48 hr. Steady state was not reached in 48 hr with PCE or HCE, although blood levels of PCE were probably close to steady-state values. Dermal flux estimates (based on branchial efflux) for TCE, PCE, and HCE were two to four times greater in catfish than in trout. Arterial blood concentrations of each compound were three to six times greater in catfish. These observations are indicative of greater flux across catfish skin, augmented by higher blood:water chemical partitioning. Trout skin is covered with scales and has no taste buds, while catfish skin does not possess scales and has numerous taste bud papillae. Both scales and taste bud papillae originate in the dermis and extend to the skin surface through the epidermis. In catfish these taste buds may offer channels through which chemicals diffuse across the epidermis to the more vascularized dermis. A comparison of dermal and branchial uptake was made by estimating zero-time dermal and branchial fluxes for all three chloroethanes. The mean dermal fluxes for TCE, PCE, and HCE ranged from 1.4 to 2.8, 1.8 to 3.6, and 1.4 to 3.2% of the total flux (branchial plus dermal) in rainbow trout and channel catfish, respectively. This research demonstrates that dermal absorption of waterborne chemicals occurs in large adult fish and results in

  3. Hormonal sex manipulation and evidence for female homogamety in channel catfish.

    PubMed

    Davis, K B; Simco, B A; Goudie, C A; Parker, N C; Cauldwell, W; Snellgrove, R

    1990-05-01

    The mechanism of sex determination in channel catfish Ictalurus punctatus was evaluated by hormonal and genetic methods. Aromatizable and nonaromatizable androgens, as well as an estrogen, caused feminization in fish fed steroids for 21 days after yolk-sac absorption. The effectiveness of 60 micrograms of ethynyltestosterone/g food decreased markedly when the experimental feeding period was shortened and was ineffective when the treatment lasted less than 12 days. Females from all-female populations produced by treatment with sex hormones were mated with normal males resulting in nine spawns with a sex ratio different from 1:1. The sex ratios were statistically similar to 3 male: 1 female in five spawns, both 2:1 and 3:1 in two spawns, and 2:1 in two spawns. These data are consistent with a model for female homogametic sex determination in channel catfish and suggest that the YY equivalent genotype is viable. PMID:2354764

  4. Prevalence and characteristics of Aeromonas species isolated from processed channel catfish.

    PubMed

    Wang, C; Silva, J L

    1999-01-01

    From August 1994 to May 1995, 238 channel catfish fillets collected from three processing plants in the Mississippi Delta at four time periods were tested for the presence of Aeromonas species. Identification of Aeromonas spp. was accomplished using an automated Vitek bioassay system with gram-negative and nonfermenter cards. Approximately 36.1% were positive for A. hydrophila, 35.7% for A. sobria, and 10.9% for A. caviae. All three Aeromonas spp. were found in all three processing plants, and the incidence of A. hydrophila contamination appeared to be higher in summer than other seasons. Eighty-six percent of the Aeromonas isolates were hemolytic on 5% sheep blood agar plates. Most isolates were susceptible to chloramphenicol, neomycin, streptomycin, and trimethoprim-sulfamethoxazole and resistant to ampicillin and bacitracin. Results suggest that Aeromonas spp. are prevalent in processed channel catfish, and most isolates are hemolytic and resistant to ampicillin and bacitracin. PMID:9921825

  5. Identification and mucosal expression analysis of cathepsin B in channel catfish (Ictalurus punctatus) following bacterial challenge.

    PubMed

    Li, Chao; Song, Lin; Tan, Fenghua; Su, Baofeng; Zhang, Dongdong; Zhao, Honggang; Peatman, Eric

    2015-12-01

    The mucosal surfaces of fish (skin, gill and intestine) constitute the primary line of defense against pathogen invasion. Although the importance of fish mucosal surfaces as the first barriers against pathogens cannot be overstated, the knowledge of teleost mucosal immunity are still limited. Cathepsin B, a lysosomal cysteine protease, is involved in multiple levels of physiological and biological processes, and playing crucial roles for host immune defense against pathogen infection. In this regard, we identified the cathepsin B (ctsba) of channel catfish and investigated the expression patterns of the ctsba in mucosal tissues following Edwardsiella ictaluri and Flavobacterium columnare challenge. Here, catfish ctsba gene was widely expressed in all examined tissues with the lowest expression level in muscle, and the highest expression level in trunk kidney, followed by spleen, gill, head kidney, intestine, liver and skin. In addition, the phylogenetic analysis showed the catfish ctsba had the strongest relationship to zebrafish. Moreover, the ctsba showed a general trend of up-regulated in mucosal tissues following both Gram-negative bacterial challenge. Taken together, the increased expression of ctsba in mucosal surfaces indicated the protective function of ctsba against bacterial infection, and the requirement for effective clearance of invading bacteria. Further studies are needed, indeed, to expand functional characterization and examine whether ctsba may play additional physiological and biological roles in catfish mucosal tissues. PMID:26497091

  6. Effect of bait and gear type on channel catfish catch and turtle bycatch in a reservoir

    USGS Publications Warehouse

    Cartabiano, Evan C.; Stewart, David R.; Long, James M.

    2014-01-01

    Hoop nets have become the preferred gear choice to sample channel catfish Ictalurus punctatus but the degree of bycatch can be high, especially due to the incidental capture of aquatic turtles. While exclusion and escapement devices have been developed and evaluated, few have examined bait choice as a method to reduce turtle bycatch. The use of Zote™ soap has shown considerable promise to reduce bycatch of aquatic turtles when used with trotlines but its effectiveness in hoop nets has not been evaluated. We sought to determine the effectiveness of hoop nets baited with cheese bait or Zote™ soap and trotlines baited with shad or Zote™ soap as a way to sample channel catfish and prevent capture of aquatic turtles. We used a repeated-measures experimental design and treatment combinations were randomly assigned using a Latin-square arrangement. Eight sampling locations were systematically selected and then sampled with either hoop nets or trotlines using Zote™ soap (both gears), waste cheese (hoop nets), or cut shad (trotlines). Catch rates did not statistically differ among the gear–bait-type combinations. Size bias was evident with trotlines consistently capturing larger sized channel catfish compared to hoop nets. Results from a Monte Carlo bootstrapping procedure estimated the number of samples needed to reach predetermined levels of sampling precision to be lowest for trotlines baited with soap. Moreover, trotlines baited with soap caught no aquatic turtles, while hoop nets captured many turtles and had high mortality rates. We suggest that Zote™ soap used in combination with multiple hook sizes on trotlines may be a viable alternative to sample channel catfish and reduce bycatch of aquatic turtles.

  7. Efficacy of hydrogen peroxide to control saprolegniasis on channel catfish (Ictalurus punctatus) eggs

    USGS Publications Warehouse

    Rach, J.J.; Valentine, J.J.; Schreier, T.M.; Gaikowski, M.P.; Crawford, T.G.

    2004-01-01

    The efficacy of hydrogen peroxide to control mortality associated with saprolegniasis in channel catfish (Ictalurus punctatus) eggs was evaluated at the Lost Valley State Fish Hatchery (Warsaw, MO). Two efficacy trials were conducted. In Trial 1, channel catfish eggs in their natural gelatinous matrix were treated with hydrogen peroxide at 0, 500, and 750 mg l(-1). Channel catfish eggs in Trial 2 had the gelatinous matrix removed before treatment with hydrogen peroxide at 0 and 500 mg l(-1). Each treatment regimen was tested in triplicate and each egg jar contained similar to 17,400 eggs. Hydrogen peroxide was administered as a 15-min flow-through treatment applied once daily for a total of six applications. Control jars were similarly treated with culture water. Samples of exposure water were collected during each treatment and analyzed to verify actual treatment concentrations. Hydrogen peroxide treatment efficacy was assessed by comparing the percent egg hatch in the treatment group to the untreated control group in each trial. Mean percent hatch in Trial I was 44% (control), 54% (500 mg l(-1)), and 69% (750 mg l(-1)). Hydrogen peroxide treatment at either 500 or 750 mg l(-1) significantly (P<0.01) increased the percent hatch compared to the untreated control group. In Trial 2, hydrogen peroxide treatment at 500 mg l(-1) significantly (P<0.01) increased the percent egg hatch (67%) relative to the untreated controls (57%). Hydrogen peroxide treatment reduced egg mortality and increased the percent hatch of channel catfish eggs regardless of whether eggs were incubated in the gelatinous matrix or without the matrix in comparison to the untreated control. (C) 2004 Elsevier B.V. All rights reserved.

  8. Acute toxicity of nitrofurazone to channel catfish Ictalurus punctatus, and goldfish, Carassius auratus

    SciTech Connect

    Wise, M.L.; Stiebel, C.L.; Grizzle, J.M.

    1987-01-01

    Nitrofurazone (5-nitro-2-furaldehyde semicarbazone) is a nitrofuran, a group of organic compounds which have inhibitory activity against many Gram-negative and Gram-positive bacteria and against some protozoan parasites. Although not approved by the United States Food and Drug Administration for use with food fish, nitrofurazone has been found effective in fish against external and internal infections by various species of Aeromonas, Pseudomonas and myxobacteria and can be administered either as a food additive or as a bath treatment. Attempts to control the microsporidian parasite Pleistophora ovariae in golden shiners, Notemigonus crysoleucas, with nitrofurazone met with equivocal results. The following experiment was performed to determine acute toxicity, including lesions, of nitrofurazone to channel catfish, Ictalurus punctatus, and goldfish, carassius auratus, fingerlings. Toxicity of nitrofurazone to channel catfish was determined with low dissolved oxygen concentrations (2 mg/L) to simulate conditions frequently encountered in channel catfish culture. Information abut toxic levels of drugs and the lesions occurring in exposed fish is important to determine the safety of treatment levels and the effects of toxic concentrations.

  9. Novel transcripts of the estrogen receptor α gene in channel catfish

    USGS Publications Warehouse

    Patino, Reynaldo; Xia, Zhenfang; Gale, William L.; Wu, Chunfa; Maule, Alec G.; Chang, Xiaotian

    2000-01-01

    Complementary DNA libraries from liver and ovary of an immature female channel catfish were screened with a homologous ERα cDNA probe. The hepatic library yielded two new channel catfish ER cDNAs that encode N-terminal ERα variants of different sizes. Relative to the catfish ERα (medium size; 581 residues) previously reported, these new cDNAs encode Long-ERα (36 residues longer) and Short-ERα (389 residues shorter). The 5′-end of Long-ERα cDNA is identical to that of Medium-ERα but has an additional 503-bp segment with an upstream, in-frame translation-start codon. Recombinant Long-ERα binds estrogen with high affinity (Kd = 3.4 nM), similar to that previously reported for Medium-ERα but lower than reported for catfish ERβ. Short-ERα cDNA encodes a protein that lacks most of the receptor protein and does not bind estrogen. Northern hybridization confirmed the existence of multiple hepatic ERα RNAs that include the size range of the ERα cDNAs obtained from the libraries as well as additional sizes. Using primers for RT-PCR that target locations internal to the protein-coding sequence, we also established the presence of several ERα cDNA variants with in-frame insertions in the ligand-binding and DNA-binding domains and in-frame or out-of-frame deletions in the ligand-binding domain. These internal variants showed patterns of expression that differed between the ovary and liver. Further, the ovarian library yielded a full-length, ERα antisense cDNA containing a poly(A) signal and tail. A limited survey of histological preparations from juvenile catfish by in situ hybridization using directionally synthesized cRNA probes also suggested the expression of ERα antisense RNA in a tissue-specific manner. In conclusion, channel catfish seemingly have three broad classes of ERα mRNA variants: those encoding N-terminal truncated variants, those encoding internal variants (including C-terminal truncated variants), and antisense mRNA. The sense variants may

  10. Induction and characterization of hepatic metallothionein expression from cadmium-induced channel catfish (Ictalurus punctatus)

    SciTech Connect

    Zhang, Y.S.; Schlenk, D.

    1995-08-01

    Two low-molecular-weight proteins of approximately 10,000 daltons (DA) were isolated from the hepatic tissue of catfish intraperitoneally injected with CdCl{sub 2} (5 mg/kg). Each protein was significantly induced following Cd treatment. Bound Cd, co-eluted from DEAE anion-exchange HPLC with rabbit hepatic metallothioneins I and II, did not possess UV absorption indicative of aromatic amino acids and corresponded to the expression of two mRNA transcripts of approximately 400 base pairs, which hybridized to a full-length cDNA probe to winter flounder metallothionein (MT). Because these data indicated that these two low molecular weight proteins were hepatic MT isoforms, induction of MT mRNA expression was studied in livers of channel catfish exposed to aqueous CdCl{sub 2} utilizing the ribonuclease protection assay. Following a 7-d exposure to 10, 50, and 100 {micro}g/L CdCl{sub 2}, expression of two MT mRNAs increased in a dose-dependent manner (MT-a, r{sup 2} = 0.95; MT-b, r{sup 2} = 0.98). Each transcript was maximally induced 48 h after exposure to 100 {micro}g/L CdCl{sub 2} and then gradually declined to approximately 20% above controls after 96 h and maintained this level of expression for 7 d. Although MT expression is rapidly induced in channel catfish by Cd, and MT mRNA measurement is a sensitive indicator of MT induction, the use of hepatic MT of channel catfish may not be a sensitive bioindicator of short-term, low-level aqueous exposures of Cd.

  11. Reductions in Susceptibility of Channel Catfish (Ictalutus punctatus) to Enteric Septicemia of Catfish (ESC) Through Two Generations of Selection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Improvement of disease resistance in aquaculture species through selective breeding has had some success. At the USDA-ARS Catfish Genetics Research Unit, Stoneville, MS, a multitrait selection index was utilized to select for growth, carcass yield, and resistance to enteric septicemia of catfish (...

  12. Oral vaccination of channel catfish against enteric septicemia of catfish (ESC) using a live attenuated Edwardsiella ictaluri isolate

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Enteric septicemia of catfish (ESC), caused by Edwardsiella ictaluri, is the most problematic bacterial disease affecting catfish aquaculture in the southeastern United States. Efforts to develop an effective ESC vaccine have had limited industrial success. In commercial settings, ESC vaccines are t...

  13. Oral vaccination of channel catfish against enteric septicemia of catfish using a live attenuated Edwardsiella ictaluri isolate

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Enteric septicemia of catfish (ESC), caused by Edwardsiella ictaluri, is the most problematic bacterial disease affecting catfish aquaculture in the southeastern United States. Efforts to develop an effective ESC vaccine have had limited industrial success. In commercial settings, ESC vaccines are...

  14. Efficacy of Hydrogen Peroxide in Controlling Mortality Associated with External Columnaris on Walleye and Channel Catfish Fingerlings

    USGS Publications Warehouse

    Rach, J.J.; Schleis, S.M.; Gaikowski, M.; Johnson, Aaron H.

    2003-01-01

    The efficacy of hydrogen peroxide in controlling mortality associated with external columnaris infections on walleye Sander vitreus (formerly Stizostedion vitreum) and channel catfish Ictalurus punctatus fingerlings was evaluated in two on-site trials. Microscopic examination of skin scrapes before treatment confirmed the presence of bacteria indicative of columnaris disease. In separate trials, walleyes (12 g) and channel catfish (2.3 g) were exposed to a total of three every-other-day hydrogen peroxide treatments of 0, 50, 75, or 100 mg/L for 60 min. Each treatment regimen was tested in triplicate, and each replicate contained 13 walleyes or 26 channel catfish. Hydrogen peroxide exposures of 50 mg/L for 60 min significantly increased walleye survival relative to that at other treatment concentrations, including the controls. Exposures of 50, 75, or 100 mg/L of hydrogen peroxide for 60 min significantly increased channel catfish survival in comparison with that of controls. These trials indicated that hydrogen peroxide treatments effectively reduce mortality in walleyes and channel catfish infected with external columnaris.

  15. Missing the target: DNAk is a dominant epitope in the humoral immune response of channel catfish (Ictalurus punctatus) to Flavobacterium columnare

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Vaccination remains a viable alternative for bacterial disease protection in fish; however additional work is required to understand the mechanisms of adaptive immunity in the channel catfish. To assess the humoral immune response to Flavobacterium columnare; a group of channel catfish were first im...

  16. Comparison of growth body composition and stress response of USDA 103 USDA 403 industry and fast growing lines of channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish selected solely for fast growth (fast growing) were compared to USDA103, USDA403, and industry pool groups of channel catfish for growth, body composition, and stress response. All fish were fed daily for 8 wks followed by an acute 10-minute dewatering stress. By wk 4, feed intake ...

  17. EFFECTS OF SHORT-TERM FEED RESTRICTION ON PRODUCTION, PROCESSING, AND BODY SHAPE TRAITS IN MARKET-WEIGHT CHANNEL CATFISH, ICTALURUS PUNCTATUS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Effects of feed restriction on channel catfish production, processing, and body shape traits were determined. Channel catfish (mean weight = 0.77 kg) were stocked in 0.04 ha ponds and assigned to three feeding regimes: fed daily to satiation, fed once weekly to satiation, and not fed for a 4-week t...

  18. The effect of luteinizing hormone releasing hormone analog regime and stage of oocyte maturity for induced ovulation of channel catfish Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The effective LHRHa (luteinizing hormone releasing hormone analog) dose based on the gonadal maturity of channel catfish, Ictalurus punctatus to optimize channel x blue hybrid catfish production was evaluated in 4 trials (twice in early part of the season and twice in the peak spawning season) in a ...

  19. Effect of carp pituitary extract and luteinizing hormone releasing analog hormone on reproductive indices and spawning of 3-year-old channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The efficacy of carp pituitary extract (CPE) and luteinizing hormone releasing hormone (LHRHa) treatments to induce spawning in young-adult channel catfish undergoing first oogenesis just prior to the spawning season was evaluated in four commercial strains of channel catfish. Prior to injection of ...

  20. An AFLP-based genetic linkage map of channel catfish (Ictalurus punctatus) constructed by using an interspecific hybrid resource family.

    PubMed Central

    Liu, Zhanjiang; Karsi, Attila; Li, Ping; Cao, Dongfeng; Dunham, R

    2003-01-01

    Catfish is the major aquaculture species in the United States. The hybrid catfish produced by crossing channel catfish females with blue catfish males exhibit a number of desirable production traits, but their mass production has been difficult. To introduce desirable genes from blue catfish into channel catfish through introgression, a genetic linkage map is helpful. In this project, a genetic linkage map was constructed using amplified fragment length polymorphism (AFLP). A total of 607 AFLP markers were analyzed using 65 primer combinations and an interspecific backcross resource family. A total of 418 AFLP markers were assigned to 44 linkage groups. Among the remaining 189 markers, 101 were not used because of significant segregation distortion, 29 were unlinked, and 59 were eliminated because they span very large distances. The 418 AFLP markers covered 1593 cM Kosambi. The AFLP markers showed a high level of clustering that appears to be related to certain primer combinations. This linkage map will serve as the basis for mapping a greater number of markers to provide a map with high enough resolution for it to be useful for selective breeding programs using introgression. PMID:14573480

  1. Phylogeny of immune recognition: antigen processing/presentation in channel catfish immune responses to hemocyanins.

    PubMed

    Vallejo, A N; Miller, N W; Jørgensen, T; Clem, L W

    1990-10-15

    Studies were conducted to address the role(s) of antigen (Ag) processing/presentation in channel catfish immune responses. Vigorous and specific secondary in vitro proliferative and antibody (Ab) responses were obtained to keyhole limpet and Limulus polyphemus hemocyanins with peripheral blood leukocytes (PBL) from catfish previously primed in vivo with Ag. In addition, such antigen-specific in vitro proliferative and Ab responses were efficiently elicited by antigen-pulsed and subsequently paraformaldehyde-fixed autologous PBL used as putative antigen-presenting cells (APC) but not by APC fixed prior to Ag pulsing. Treatment of these putative APC with lysosomotropic agents, protease inhibitors, or the ionophore monensin prior to or during pulsing with Ag significantly inhibited both in vitro responses. Furthermore, the use of radiolabeled protein indicated that both untreated and inhibitor-treated PBL but not erythrocytes take up Ag; however, only untreated PBL were able to degrade Ag. Immune restriction was indicated by the use of allogeneic PBL as APC in that only strong MLRs were generated with no detectable antibodies produced in vitro. Finally, the employment of isolated leukocyte subpopulations demonstrated that both catfish B (sIg+) lymphocytes and monocytes were efficient Ag presentors. PMID:2208303

  2. Differential localization of putative amino acid receptors in taste buds of the channel catfish, Ictalurus punctatus.

    PubMed

    Finger, T E; Bryant, B P; Kalinoski, D L; Teeter, J H; Böttger, B; Grosvenor, W; Cagan, R H; Brand, J G

    1996-09-01

    The taste system of catfish, having distinct taste receptor sites for L-alanine and L-arginine, is highly sensitive to amino acids. A previously described monoclonal antibody (G-10), which inhibits L-alanine binding to a partial membrane fraction (P2) derived from catfish (Ictalurus punctatus) taste epithelium, was found in Western blots to recognize a single band, at apparent MW of 113,000 D. This MW differs from the apparent MW for the presumed arginine receptor identified previously by PHA-E lectin affinity. In order to test whether PHA-E lectin actually reacts with the arginine-receptor, reconstituted membrane proteins partially purified by PHA-E affinity were used in artificial lipid bilayers. These reconstituted channels exhibited L-arginine-activated activity similar to that found in taste cell membranes. Accordingly, we utilized the PHA-E lectin and G-10 antibody as probes to differentially localize the L-alanine and L-arginine binding sites on the apical surface of catfish taste buds. Each probe labels numerous, small (0.5-1.0 micron) patches within the taste pore of each taste bud. This observation suggests that each bud is not tuned to a single taste substance, but contains putative receptor sites for both L-arginine and L-alanine. Further, analysis of double-labeled tissue reveals that the PHA-E and G-10 sites tend to be separate within each taste pore. These findings imply that in catfish, individual taste cells preferentially express receptors to either L-arginine or L-alanine. In addition, PHA-E binds to the apices of solitary chemoreceptor cells in the epithelium, indicating that this independent chemoreceptor system may utilize some receptor sites similar to those in taste buds. PMID:8876468

  3. Odorant receptors activated by amino acids in sensory neurons of the channel catfish Ictalurus punctatus.

    PubMed

    Ivanova, T T; Caprio, J

    1993-12-01

    Odorant receptors activated by amino acids were investigated with patch-clamp techniques in olfactory receptor neurons of the channel catfish, Ictalurus punctatus. The L-isomers of alanine, norvaline, arginine, and glutamate, known to act predominantly on different olfactory receptor sites, activated nondesensitizing inward currents with amplitudes of -2.5 to -280 pA in olfactory neurons voltage-clamped at membrane potentials of -72 or -82 mV. Different amino acids were shown to induce responses in the same sensory neurons; however, the amplitude and the kinetics of the observed whole cell currents differed among the stimuli and may therefore reflect activation of different amino acid receptor types or combinations of receptor types in these cells. Amino acid-induced currents appeared to have diverse voltage dependence and could also be classified according to the amplitude of the spontaneous channel fluctuations underlying the macroscopic currents. A mean single-channel conductance (gamma) of 360 fS was estimated from small noise whole-cell currents evoked by arginine within the same olfactory neuron in which a mean gamma value of 23.6 pS was estimated from 'large noise' response to norvaline. Quiescent olfactory neurons fired bursts of action potentials in response to either amino acid stimulation or application of 8-Br-cyclic GMP (100 microM), and voltage-gated channels underlying generation of action potentials were similar in these neurons. However, in whole-cell voltage-clamp, 8-Br-cyclic GMP evoked large rectangular current pulses, and single-channel conductances of 275, 220, and 110 pS were obtained from the discrete current levels. These results suggest that in addition to the cyclic nucleotide-gated transduction channels, olfactory neurons of the channel catfish possess a variety of odor receptors coupled to different types of transduction channels. PMID:8133240

  4. Odor stimuli trigger influx of calcium into olfactory neurons of the channel catfish.

    PubMed

    Restrepo, D; Miyamoto, T; Bryant, B P; Teeter, J H

    1990-09-01

    Olfactory transduction is thought to be mediated by a G protein-coupled increase in intracellular adenosine 3',5'-monophosphate (cAMP) that triggers the opening of cAMP-gated cation channels and results in depolarization of the plasma membrane of olfactory neurons. In olfactory neurons isolated from the channel catfish, Ictalurus punctatus, stimulation with olfactory stimuli (amino acids) elicits an influx of calcium that leads to a rapid increase in intracellular calcium. In addition, in a reconstitution assay a plasma membrane calcium channel has been identified that is gated by inositol-1,4,5-trisphosphate (IP3), which could mediate this calcium influx. Together with previous studies indicating that stimulation with olfactory stimuli leads to stimulation of phosphoinositide turnover in olfactory cilia, these data suggest that an influx of calcium triggered by odor stimulation of phosphoinositide turnover may be an alternate or additional mechanism of olfactory transduction. PMID:2168580

  5. Glossopharyngeal taste responses of the channel catfish to binary mixtures of amino acids.

    PubMed

    Ogawa, K; Caprio, J

    2000-10-01

    This study examines the neural processing of binary mixtures in the glossopharyngeal (IX) taste system of the channel catfish, Ictalurus punctatus, and finds that the nature of the components of a mixture determines the intensity of the neural response to it. Taste buds in fish innervated by IX are located along the gill rakers of the first gill arch and rostral floor of the oral cavity, and function primarily in the consummatory phase of feeding behavior; however, few studies of IX taste responses have been reported in any species of teleost. Here, we report IX taste responses to eight different binary mixtures of amino acids whose components were adjusted to be approximately equipotent in electrophysiological recordings. Four binary (group I) mixtures whose components were indicated from prior electrophysiological cross-adaptation experiments to bind to independent receptor sites resulted in significantly larger (22% average increase) integrated IX taste activity than four other (group II) binary mixtures whose components were indicated to bind to the same or highly cross-reactive receptor sites. These results are similar to those observed previously from facial nerve recordings in channel catfish, and to olfactory and taste responses in other vertebrate and invertebrate species. The group I results help to explain behavioral observations that chemical mixtures of chemosensory stimuli are often more stimulatory than their individual components. PMID:11015321

  6. Inhibition of nitrite-induced toxicity in channel catfish by calcium chloride and sodium chloride

    USGS Publications Warehouse

    Tommasso J.R., Wright, M. I.; Simco, B.A.; Davis, K.B.

    1980-01-01

    Environmental chloride has been shown to inhibit methemoglobin formation in fish, thereby offering a protective effect against nitrite toxicity. Channel catfish (Ictalurus punctatus) were simultaneously exposed to various environmental nitrite and chloride levels (as either CaCl2 or NaCl) in dechlorinated tap water (40 mg/L total hardness, 47 mg/L alkalinity, 4 mg/L chloride, pH = 6.9-7.1, and temperature 21-24°C). Methemoglobin levels in fish simultaneously exposed to 2.5 mg/L nitrite and up to 30 mg/L chloride as either CaCl2 or NaCl were similar but significantly lower than in unprotected fish. Exposure to 10 mg/L nitrite and 60 mg/L chloride resulted in methemoglobin levels similar to those of the controls; most unprotected fish died. Fish exposed to 10 mg/L nitrite had significantly lower methemoglobin levels when protected with 15.0 mg/L chloride as CaCl2 than with NaCl. Fish exposed to nitrite in the presence of 60 mg/L chloride (as either CaCl2 or NaCl) had similar 24-h LC50 values that were significantly elevated above those obtained in the absence of chloride. Calcium had little effect on tolerance to nitrite toxicity in channel catfish in contrast to its large effect reported in steelhead trout (Salmo gairdneri).

  7. Uptake, tissue distribution, and metabolism of malachite green in the channel catfish (Ictalurus punctatus)

    USGS Publications Warehouse

    Plakas, S.M.; El Said, K. R.; Stehly, G.R.; Gingerich, W.H.; Allen, J.L.

    1996-01-01

    The disposition of malachite green was determined in channel catfish (Ictalurus punctatus) after intravascular dosing (0.8 mg . Kg-1) or waterborne exposure (0.8 mg . L-1 for 1 h). After intravascular dosing, mean plasma concentrations of the parent compound exhibited a triphasic decline with a terminal elimination half-life of 6.2 h. Malachite green was rapidly absorbed and concentrated in the tissues during waterborne exposure. The rate of accumulation was directly related to pH of the exposure water. After waterborne exposure, elimination of the parent compound from plasma also was triphasic with a terminal half-life of 4.7 h. In muscle, the half-life of the parent compound was approximately 67 h. Malachite green and its metabolites were widely distributed in all tissues. In fish exposed to C-14-labeled malachite green, total drug equivalent concentrations were highest in abdominal fat and lowest in plasma. Malachite green was rapidly and extensively metabolized to its reduced form, leucomalachite green, which was slowly eliminated from the tissues. Leucomalachite green is an appropriate target analyte for monitoring exposure of channel catfish to this drug.

  8. Intestinal synthesis and absorption of vitamin B-12 in channel catfish

    SciTech Connect

    Limsuwan, T.; Lovell, R.T.

    1981-12-01

    A feeding experiment conducted in a controlled environment and using a vitamin B12-deficient, but otherwise nutritionally complete, purified diet revealed that intestinal microorganisms in channel catfish synthesized approximately 1.4 ng of vitamin B12 per gram of bodyweight per day. Removal of cobalt from the diet or supplementation with an antibiotic (succinylsulfathiazole) significantly reduced the rate of intestinal synthesis and liver stores of vitamin B12. Radiolabeled vitamin B12 in the blood, liver, kidneys, and spleen of fish fed 60Co in the diet indicated that the intestinally synthesized vitamin was absorbed by the fish. The primary route of absorption was directly from the digestive tract into the blood because coprophagy was prevented in the rearing aquariums and the amount of vitamin B12 dissolved in the aquarium water was too low for gill absorption. Dietary supplementation of vitamin B12 was not necessary for normal growth and erythrocyte formation in channel catfish in a 24-week feeding period. A longer period, however, may have caused a vitamin deficiency since liver-stored vitamin B 12 decreased between the 2nd and 24th weeks.

  9. Evaluation of a loop-mediated isothermal amplification method for rapid detection of channel catfish Ictalurus punctatus important bacterial pathogen Edwardsiella ictaluri.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish Ictalurus punctatus infected with Edwardsiella ictaluri results in $40 - 50 million annual losses in profits to catfish producers. Early detection of this pathogen is necessary for disease control and reduction of economic loss. In this communication, the loop-mediated isothermal a...

  10. Cocaine- and Amphetamine-Regulated Transcript (CART) Messenger RNA Expression is Regulated by Food Intake in the Brain of the Channel Catfish (Ictalurus punctatus)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Increased growth is included as a selection criterion at the USDA Catfish Genetics Research Unit, Stoneville, MS, USA. Faster growth of selected channel catfish is typically attributed to their ability to consume more feed. While endocrine and neural factors and mechanisms that regulate feed intak...

  11. The effects of essential oils BIOMIN PEP MGE on weight gain feed conversion ratio and survival of channel catfish Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We examined the effects of matrix encapsulated essential oils (Biomin® P.E.P. MGE) on weight gain, specific growth rate (SGR), feed conversion ratio (FCR), and survival of channel catfish. Five hundred catfish (32.4 ± 1.7 g/fish) were randomly assigned to two treatments with five replicate tanks/tre...

  12. Influence of strain of sire on channel x blue F1 hybrid catfish embryo production and fingerling performance under commercial farm conditions.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel x blue hybrid catfish is presently the desired aquaculture species in US farm-raised catfish industry. Even though production and performance has improved over the last two decades, several production and performance parameters needs to be improved to maximize the genetic potential of this ...

  13. Spleen size and plasma levels of mannose binding lectin in channel catfish Ictalurus punctatus families exhibiting different susceptibilities to Flavobacterium columnare and Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Two major problems in the channel catfish (Ictalurus punctatus) aquaculture industry have been high disease losses to enteric septicemia of catfish (ESC), caused by the bacterium Edwardsiella ictaluri and columnaris disease, caused by the bacterium Flavobacterium columnare. Methods to control these...

  14. Comparing the efficacy of four immersion flush and static hydorgen peroxide and copper sulfate treatments on channel catfish eggs infected with water molds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Water mold infestations on channel catfish eggs lower the hatch rate (egg survival) and ultimately the number of catfish fry available for stocking in production ponds. This study compared the potential of two hydrogen peroxide (HP) and two copper sulfate pentahydrate (CSP) treatments to increase c...

  15. In vitro evaluation of the antimicrobial agent AquaFrin(TM) as a bactericide and selective algicide for use in channel catfish (Ictalurus punctatus) aquaculture

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Producers of pond-raised channel catfish in the southeastern United States can experience huge economic losses due to the bacterial diseases enteric septicemia of catfish (ESC) and columnaris and to the presence of the certain odor-producing cyanobacteria in production ponds that result in “off-flav...

  16. Hatch rate of channel catfish Ictalurus punctatus (Rafinesque 1818) eggs treated with 100 mg L-1 copper sulphate pentahydrate. Aquaculture Research

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Catfish hatcheries use copper sulfate (CuSO4) as an economical control for saprolegniasis on eggs. This study determines hatch rate of channel catfish eggs in hatching troughs containing 23.8°C flow-through well water when treated with 100 mg/L CuSO4 (ten times the proposed therapeutic dose). Eggs...

  17. Growth and Feed Efficiency of Juvenile Channel Catfish Ictalurus punctatus Fed Diets Containing Various Levels of Fish Meal at Different Water Temperatures

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Unlike warm-blooded animals, channel catfish do not feed consistently when water temperatures are below 20°C and generally stop eating at temperatures of 10°C and below. Many catfish producers choose not to feed during winter months for various reasons, one of which is that it is difficult to see a...

  18. Effects of Dietary Protein Concentration and L-carnitine on Growth, Processing Yield, and Body Composition of Channel X Blue Catfish Hybrids

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A study was conducted in earthen ponds to evaluate effects of dietary protein concentration and L-carnitine supplementation on production and processing traits of channel catfish × blue catfish hybrids. Hybrid fingerlings, mean initial weight = 66 g, were stocked into 20, 0.04-ha earthen ponds at a...

  19. Spleen index and mannose-binding lectin levels in four channel catfish Ictalurus punctatus families exhibiting different susceptibilities to Flavobacterium columnare and Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Edwardsiella ictaluri and Flavobacterium columnare are two bacterial pathogens that impact the channel catfish Ictalurus punctatus aquaculture. Some progress has been made at the USDA-ARS Catfish Genetics Research Unit in selectively breeding for resistance to E. ictaluri; however, the susceptibili...

  20. Effect of supplemental taurine on juvenile channel catfish Ictalurus punctatus growth and survival after challenge with Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Juvenile channel catfish, Ictalurus punctatus, were fed a basal diet that contained major protein (soybean meal, cottonseed meal) and energy (ground corn grain) ingredients that were derived from plant sources. Plant-source ingredients are considered to be low (< 5 ppm) for taurine content. In add...

  1. Cross-protection elicited in channel catfish (Ictalurus punctatus Rafinesque) immunized with a low dose of virulent Edwardsiella ictaluri strains

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cross-protection of fingerling channel catfish (Ictalurus punctatus Rafinesque) by immersion immunization and challenge exposure to six E. ictaluri strains (AL-93-58, AL-93-75, S-94-1051, NJ #3, EILO and ATCC-33202) originating from out breaks of disease in disparate geographic locations was examine...

  2. Replacement of Soybean Meal in Channel Catfish, Ictalurus punctatus, Diets with Cottonseed Meal and Distillers Dried Grains with Solubles

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Two pond experiments were conducted to evaluate cottonseed meal (CSM), distiller’s dried grains with solubles (DDGS), and supplemental lysine as replacements for soybean meal (SBM) in channel catfish diets. In Experiment 1, fish fed diets in which SBM was totally replaced with CSM gained similar wei...

  3. Identification and expression profile of multiple genes in channel catfish fry ten minutes after modified live Flavobacterium columnare vaccination

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Using PCR-select subtractive cDNA hybridization technique, 32 expressed sequence tags (ESTs) were isolated from 96 clones of a channel catfish (Ictalurus punctatus) fry subtractive library ten minutes post vaccination with a modified live Flavobacterium columnare vaccine. The transcription levels of...

  4. Molecular characterization, phylogenetic analysis and expression patterns of five protein arginine methyltransferase genes of channel catfish, Ictalurus punctatus (Rafinesque)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Protein arginine methylation, catalyzed by protein arginine methyltransferases (PRMT), has recently emerged as an important modification in the regulation of gene expression. In this communication, we identified and characterized the channel catfish orthologs to human PRMT 1, 3, 4 and 5, and PRMT4 ...

  5. Impacts of Bolbophorus damnificus digenea: Bolbophoridae on production characteristics of channel catfish, Ictalurus punctatus, raised in experimental ponds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bolbophorus damnificus was recognized as a threat to channel catfish, Ictalurus punctatus, production in Louisiana and Mississippi in 1997 and 1999, respectively. Severe to moderate outbreaks (=34% prevalence) are correlated to heavy losses, but impact of mild outbreaks with low prevalence (<33%) wa...

  6. GROWTH AND SURVIVAL OF CHANNEL CATFISH AND YELLOW PERCH EXPOSED TO LOWERED CONSTANT AND DIURNALLY FLUCTUATING DISSOLVED OXYGEN CONCENTRATIONS

    EPA Science Inventory

    Growth and survival were determined for duplicate lots of juvenile channel catfish (Ictalurus punctatus) and yellow perch (Perca flavescens) exposed for 69 and 67 days, respectively, to nearly constant dissolved oxygen (DO) concentrations at near air saturation (control), 6.5, 5....

  7. Replacement of fish meal in juvenile channel catfish, Ictalurus punctatus, diets using a yeast-derived protein source

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We examined the effects of a yeast-derived protein source (NuPro) as a replacement for menhaden fish meal on weight gain, specific growth rate (SGR), food conversion ratio (FCR), whole-body composition, and disease resistance in juvenile channel catfish. NuPro replaced 0, 20, 40, 60, 80, and 100% o...

  8. Edwardsiella tarda and Aeromonas hydrophila isolated from diseased Southern flounder (Paralichthys lethostigma) are virulent to channel catfish and Nile tilapia

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The aim of this study is to identify bacterial pathogens isolated from diseased Southern flounder and determine their virulence to channel catfish and Nile tilapia. Twenty five Gram-negative bacteria isolates were recovered from five tissues (skin lesions, brain, liver, intestine, and posterior kidn...

  9. Identification and characterization of matrix metalloproteinase-13 sequence structure and expression during embryogenesis and infection in channel catfish (Ictalurus punctatus)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Matrix metalloproteinase-13 (MMP-13), referred to as collagenase-3, is a proteolytic enzyme that plays a key role in degradation and remodelling of host extracellularmatrix proteins. The objective of this study was to characterize the MMP-13 gene in channel catfish, and to determine its pattern of e...

  10. Association of Cocaine- and Amphetamine-Regulated Transcript (CART) Messenger RNA Level, Food Intake, and Growth in Channel Catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cocaine-and Amphetamine-Regulated Transcript (CART) is a potent hypothalamic anorectic peptide in mammals and fish. We hypothesized that increased food intake is associated with changes in expression of CART mRNA within the brain of channel catfish. Objectives were to clone the CART gene, examine ...

  11. Effects of essential oil on performance characteristics, survival, processing characteristics, and fillet proximate composition of channel catfish, Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We examined the effects of matrix encapsulated essential oils (Digestarom® P.E.P. MGE) on performance, survival, processing characteristics, and fillet proximate composition of channel catfish. Fifteen one-acre ponds at the Delta-Western Research Center, Indianola, MS were stocked with approximatel...

  12. Effects of a phytogenic feed additive on susceptibility of channel catfish to Edwardsiella ictaluri and levels of mannose binding lectin

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A study was conducted to investigate the effect of a phytogenic feed additive (Digestarom® P.E.P. MGE) on growth performance and disease susceptibility to Edwardsiella ictaluri. Two hundred and fifty juvenile channel catfish (7.2 ± 0.1 g) were allotted into the following treatments: Control (float...

  13. Channel Catfish, Ictalurus punctatus Rafinesque 1818, Tetraspanin Membrane Protein Family: Characterization and Expression Analysis of CD81 cDNA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    CD81, also known as the target of an antiproliferative antibody 1 (TAPA-1), is a member of tetraspanin integral membrane protein family. This protein plays many important roles in immune functions. In this report, we characterized and analyzed expression of the channel catfish CD81 transcript. T...

  14. Identification and expression profile of multiple genes in channel catfish fry after modified live Flavobacterium columnare vaccination

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Using PCR-select subtractive cDNA hybridization technique, 32 expressed sequence tags (ESTs) were isolated from 96 clones of a channel catfish (Ictalurus punctatus) fry subtractive library ten minutes post vaccination with a modified live Flavobacterium columnare vaccine. The transcription levels of...

  15. NRAMP, TNF, TLR5, and Hepcidin Expression in Resistant and Susceptible Families of Channel Catfish Following Challenge With E. ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Real-time PCR was used to measure gene expression of Nramp, TNF, TLR5, and Hepcidin, in spleen and liver tissue from two families of channel catfish, one resistant and one susceptible to ESC, following challenge with Edwardsiella ictaluri. There were no significant differences in relative copy numbe...

  16. Increasing Fish Oil Levels in Commercial Diets Influences Hematological and Immunological Responses of Channel Catfish, Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Growth performance, immune responses and disease resistance were studied in juvenile channel catfish, Ictalurus punctatus fed a commercial diet (35.3% crude protein and 5.6% lipid) supplemented with menhaden fish oil at levels of 0, 3, 6 and 9% for 15 weeks. Dietary fish oil levels did not significa...

  17. Use of corn gluten feed and cottonseed meal to replace soybean meal in diets for pond raised channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The prices of soybean meal and corn he two most commonly used, traditional feed ingredients in channel catfish diets ave increased dramatically in recent years. Using less-expensive alternative feed ingredients to partially replace soybean meal and corn would reduce feed cost. The present study eval...

  18. Effect of Channel Catfish Ictalurus Punctatus Egg Mass Loading Rate on Performance of the “See-Saw” Incubator

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The “See-Saw” is a vertical-lift incubator that increases water circulation around and through channel catfish egg masses. Baskets in the incubator move up and down on racks in conventional hatchery troughs, raising the rack in one trough while lowering the rack in the adjacent trough. Egg masses ...

  19. Efficacy of Florfenicol for Control of Mortality Caused by Flavobacterium columnare Infection in Channel Catfish, Ictalurus punctatus (Rafinesque)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The efficacy of florfenicol against Flavobacterium columnare infection was studied in channel catfish (Ictalurus puntatus) fingerlings in 80L aquaria. Non-abraded fish were challenged by immersion on Day 0. Thirty 80 liter tanks were randomly assigned in equal number to two treatment groups, eithe...

  20. Characterization of a BAC Library from Channel Catfish Ictalurus punctatus: Indications of High Rates of Evolution Among Teleost Genomes

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The CHORI-212 bacterial artificial chromosome (BAC) library was constructed by cloning EcoRI/EcoRI partially digested DNA into the pTARBAC2.1 vector. The library has an average insert size of 161 kb, and provides 10.6-fold coverage of the channel catfish haploid genome. Screening of 32 genes using o...

  1. Use of Alaskan pollack viceral meal in diets of channel catfish to enhance the N-3 content of the fillet

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The objective of this study was to determine the potential for using APVM in practical diets of channel catfish to sustain or improve general performance while increasing the n-3 fatty acid concentration of fillets. Replacement of marine products such as menhaden fish meal and oil in diets of cultur...

  2. Virulence of Aeromonas hydrophila to channel catfish Ictalurus punctatus fingerlings in the presence and absence of bacterial extracellular products

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Virulence of three 2009 West Alabama isolates (AL09-71, AL09-72, and AL09-73) of Aeromonas hydrophila in the presence or absence of extracellular products (ECP) from overnight bacterial culture to channel catfish fingerlings (4.6 +/- 1.3g) was investigated by both bath immersion and intraperitoneal ...

  3. L-rhamnose-binding lectins (RBLs) in channel catfish, Ictalurus punctatus: Characterization and expression profiling in mucosal tissues

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Rhamnose binding-lectins (RBLs) have recently emerged as important molecules in the context of innate immunity in teleost fishes. Previously, using RNA-seq technology, we observed marked up-regulation of a RBL in channel catfish (Ictalurus punctatus) gill following a challenge with the bacterial pat...

  4. Transcriptional profiles of multiple genes in the anterior kidney of channel catfish vaccinated with an attenuated Aeromonas hydrophila

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A total of 22 uniquely expressed sequence tags (ESTs) were identified from channel catfish anterior kidney subtractive cDNA library at 12h post vaccination with an attenuated A. hydrophila (AL09-71 N+R). Of the 22 ESTs, six were confirmed to be significantly (P<0.05) induced by the vaccination. Of 8...

  5. Effects of dietary Chinese tea on growth performance, disease resistance, and fresh fatty acid profile of Channel catfish (Ictalurus punctatus).

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Six types of Chinese tea were incorporated into diets at 4 different levels ((0, 1, 2, and 4% w/w) in order to determine their effects on the growth performance, fatty acid composition of muscle lipids and resistance to Aeromonas hydrophila of channel catfish (Ictalurus punctatus) fingerlings. Each...

  6. Evaluation of potassium permanganate against an experimental subacute infection of Flavobacterium columnare in channel catfish, Icatlurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An experiment was performed to evaluate the efficacy of potassium permanganate (KMnO4) as a prophylactic and therapeutic treatment of an experimental subacute infection of Flavobacterium columnare in channel catfish, Ictalurus punctatus. Fish were cutaneously abraded and divided into five treatment...

  7. Evaluation of a 4-h static copper sulphate treatment against experimental infection of Flavobacterium columnare in channel catfish (Ictalurus punctatus)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The efficacy of copper sulfate (CuSO4) against early stages of an experimental acute infection of Flavobacterium columnare in channel catfish (Ictalurus punctatus) was evaluated. Fish were challenged, by waterborne exposure to F. columnare in an ultra-low flow-through water delivery system, and tre...

  8. The Effects of Harvesting and Hauling on the Blood Physiology and Fillet Quality of Food-Size Channel Catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Commercially produced food-size channel catfish Ictalurus punctatus that were harvested using routine procedures had significant elevations in plasma cortisol, glucose, and lactate compared to preharvest fish captured from open ponds. Blood samples were taken from 10 fish in each of the two treatmen...

  9. The effects of harvesting and hauling on the blood physiology and fillet quality of market-size channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Commercially produced adult channel catfish harvested using routine procedures had significant elevations in plasma cortisol, glucose, and lactate compared to pre-harvest fish captured from open ponds. Blood samples were taken from ten fish in each of the two treatments (liquid oxygen and paddlewhee...

  10. The effects of harvesting and hauling on the blood physiology and fillet quality of adult channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Technical Abstract: Commercially produced adult channel catfish harvested using routine procedures had significant elevations in plasma cortisol, glucose, and lactate compared to pre-harvest fish captured from open ponds. Blood samples were taken from ten fish in each of the two treatments (liquid ...

  11. The Effects of Harvesting and Hauling on the Blood Physiology and Fillet Quality of Adult Channel Catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Commercially produced adult channel catfish harvested using routine procedures had significant elevations in plasma cortisol, glucose, and lactate compared to pre-harvest fish captured from open ponds. Blood samples were taken from ten fish in each of the two treatments (liquid oxygen and paddlewhee...

  12. Dose-confirmation of copper sulfate for treating fungus on channel catfish eggs at a commercial hatchery

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study at a commercial hatchery was required by the U.S. Food and Drug Administration to provide independent substantiation of the results of a previous laboratory dose confirmation study to control fungus (Saprolegnia spp.) on channel catfish Ictalurus punctatus eggs with copper sulfate (CuSO4)...

  13. Effects of Dietary Bovine Lactoferrin on Disease Resistance, Immune Function, and Stress Resistance of Channel Catfish and Nile Tilapia

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Juvenile channel catfish (Ictalurus punctatus) and Nile tilapia (Oreochromis niloticus) were fed nutritionally complete, practical basal diets supplemented with bovine lactoferrin (Lf) at 0, 200, 400, 800, or 1600 mg/kg diet to apparent satiation twice daily for 5 and 8 weeks, respectively, to deter...

  14. The effect of feeding diets containing deoxynivalenol contaminated corn on channel catfish (Ictalurus punctatus) challenged with Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish were fed practical corn-soybean meal diets for 10 weeks that contained various weighed amounts of ground, dried field corn contaminated with 20 mg deoxynivalenol (DON)/kg. Weighed amounts of DON corn were blended with weighed amounts of ground clean corn that contained no DON (0 mg/k...

  15. Development and efficacy of novobiocin and rifampicin-resistant Aeromonas hydrophila as novel vaccines in channel catfish and Nile tilapia

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Three attenuated Aeromonas hydrophila vaccines were developed from the virulent 2009 West Alabama isolates through selection for resistance to both novobiocin and rifampicin. When channel catfish (Ictalurus punctatus) were IP injected with 4×105 colony-forming units (CFU) of the mutants, no fish die...

  16. Rhamnose-binding lectins and their ligands: Putative roles in Flavobacterium columnare pathogenesis in channel catfish Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Columnaris disease, caused by the bacterial pathogen Flavobacterium columnare, continues to be a major problem worldwide and commonly leads to tremendous losses of both wild and cultured freshwater fish, particularly in intensively farmed aquaculture species such as channel catfish. Despite its eco...

  17. Interaction between dietary levels of victamin C and E on growth and immune responses in channel catfish, Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study was conducted to evaluate the effect of dietary levels of vitamins C and E on growth performance, liver contents of vitamins C and E, hematology and immune response of channel catfish, Ictalurus punctatus. A basal practical diet containing 32% protein and 2,900 kcal DE kg-1 was supplemen...

  18. Effects of Ichthyophthirius multifiliis parasitism on the survival, hematology and bacterial load in channel catfish previously exposed to Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The effect of Ichthyophthirius multifiliis (Ich) parasitism on survival, hematology and bacterial load in channel catfish, Ictalurus punctatus, previously exposed to Edwardsiella ictaluri was studied. Fish were exposed to E. ictaluri one day prior to Ich in the following treatments: 1)- infected by...

  19. Identification of overexpressed immune-related genes in the anterior kidney of channel catfish induced by Edwardsiella ictaluri vaccination.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Using PCR-select subtractive cDNA hybridization technique, 57 expressed sequence tags (ESTs) were isolated from 240 clones of a modified live Edwardsiella ictaluri-vaccinated vs sham-vaccinated channel catfish anterior kidney subtractive library. The transcription levels of the 57 ESTs in response t...

  20. Identification and expresion profile of multiple genes in channel catfish fry ten minutes after modified live Flavobacterium columnare vaccination

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Using PCR-select subtractive cDNA hybridization technique, 32 expressed sequence tags (ESTs) were isolated from 96 clones of a channel catfish (Ictalurus punctatus) fry subtractive library ten minutes post vaccination with a modified live Flavobacterum columnare vaccine. The transcription levels of ...

  1. An Attenuated Strain of Edwardsiella ictaluri is Killed by Channel Catfish (Ictalurus punctatus) Macrophages and Confers Protection in Few Days

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Background: Edwardsiella ictaluri is able to survive inside macrophages. Several studies have demonstrated that in channel catfish protective immunity against E. ictaluri is mediated by a cellular immune response. Methods: A virulent strain and an attenuated strain (used as a modified live vaccine) ...

  2. Degradation of chitin and chitosan by a recombinant chitinase derived from a virulent Aeromonas hydrophila isolated from diseased channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A chitinase was identified in extracellular products of a virulent Aeromonas hydrophila isolated from diseased channel catfish (Ictalurus punctatus). Bioactive recombinant chitinase (rChi-Ah) was produced in Escherichia coli. Purified rChi-Ah had optimal activity at temperature of 42°C and pH 6.5. T...

  3. Identification, phylogenetic relationships, characterization and gene expression patterns of six different annexins of channel catfish (Ictalurus punctatus Rafinesque, 1818)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Annexins are Ca++-dependent phospholipid-binding proteins. They are ubiquitous in living organisms and are involved in many cellular processes. In the course of studying Edwardsiella ictaluri pathogenesis in channel catfish, we identified that six annexin expressed sequence tags (A1, A2, A4, A5, A...

  4. Comparative effects of copper sulfate or potassium permanganate on channel catfish concurrently infected with Flavobacterium columnare and Ichthyobodo necator

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An opportunistic study was conducted to determine the effects of two chemical therapeutants on channel catfish (CCF) Ictalurus punctatus concurrently infected Flavobacterium columnare and Ichthyobodo necator. Copper sulfate (CuSO4) and potassium permanganate (KMnO4) were investigated for their abil...

  5. Proteomic analysis of head kidney tissue from high and low susceptibility families of channel catfish following challenge with Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A study was performed to compare proteomic profiles of channel catfish from families with high and low susceptibility to Edwardsiella ictaluri following an immersion challenge. Total protein was isolated from head kidney samples, collected at 2 and 6 hours post exposure, and analyzed by 2-D-gel elec...

  6. Binding and Phagocytosis by Opsonized and Nonopsonized Channel Catfish Macrophages of Viable DsRed-fluorescent-labeled Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Phagocyte-mediated killing of bacterial pathogens is one of the major defensive mechanisms in fish. The binding, uptake and destruction of recombinant fluorescent protein DsRed transformed Edwardsiella ictaluri by opsonized and nonopsonized channel catfish (Ictalurus punctatus) macrophages was chara...

  7. Basal polarization of the mucosal compartment in Flavobacterium columnare susceptible and resistant channel catfish (Ictalurus punctatus).

    PubMed

    Peatman, Eric; Li, Chao; Peterson, Brian C; Straus, David L; Farmer, Bradley D; Beck, Benjamin H

    2013-12-01

    The freshwater bacterial pathogen, Flavobacterium columnare, infects a variety of ornamental and farmed fish species worldwide through mucosal attachment points on the gill and skin. While previous studies have demonstrated a chemotactic response of F. columnare to fish mucus, little is known about how host gill mucosal molecular and cellular constituents may impact rates of adhesion, tissue invasion, and ultimately, mortality. Here, we describe the use of RNA-seq to profile gill expression differences between channel catfish (Ictalurus punctatus) differing in their susceptibility to F. columnare both basally (before infection) and at three early timepoints post-infection (1 h, 2 h, and 8 h). After sequencing and de novo assembly of over 350 million 100 base-pair transcript reads, between group comparisons revealed 1714 unique genes differentially expressed greater than 1.5-fold at one or more timepoints. In the large dataset, we focused our analysis on basal differential expression between resistant and susceptible catfish as these genes could potentially reveal genetic and/or environmental factors linked with differential rates of infection. A number of critical innate immune components including iNOS2b, lysozyme C, IL-8, and TNF-alpha were constitutively higher in resistant catfish gill, while susceptible fish showed high expression levels of secreted mucin forms, a rhamnose-binding lectin previously linked to susceptibility, and mucosal immune factors such as CD103 and IL-17. Taken together, the immune and mucin profiles obtained by RNA-seq suggest a basal polarization in the gill mucosa, with susceptible fish possessing a putative mucosecretory, toleragenic phenotype which may predispose them to F. columnare infection. PMID:23895942

  8. The effects of a sublethal dose of botulinum serotype e on the swimming performance of channel catfish fingerlings.

    PubMed

    Beecham, Rachel; Thomas, Torri; Gao, Dana X; Gaunt, Patricia S

    2014-09-01

    Abstract Visceral toxicosis of catfish (VTC) is a disease of cultured Channel Catfish Ictalurus punctatus in the Mississippi Delta region and surrounding states. The etiology of VTC is associated with botulinum serotype E (BoNT/E), which causes blockage of acetylcholine release at the neuromuscular junction, leading to weakness and paralysis of skeletal muscles (including those involved in swimming). This study attempted to determine if sublethal exposure to purified BoNT/E caused reductions in swimming performance and metabolism of Channel Catfish. Catfish swimming performance was assessed on stocker-sized Channel Catfish (mean weight ± SD, 62.35 ± 2.5 g) with 10 sham-injected fish and 10 fish injected with a sublethal dose of BoNT/E. A modified Blazka-type swim chamber was used to assess swimming performance. We injected Channel Catfish with either 0.015% trypsin or 400 pg purified BoNT/E digested with 0.015% trypsin intracoelomically, then acclimated an individual catfish in the swim chamber for 17 h prior to the swimming trial. Water temperature was maintained at ∼28°C, and dissolved oxygen (DO) was between 4 and 7 mg/L. A critical swimming speed (Ucrit) protocol was followed, and DO and temperature were monitored every 2 min throughout the swim trial. Cost of transport was calculated from the oxygen consumption at each test speed (10-70 cm/s). There was a statistical difference between the Ucrits (P = 0.0034), but no differences were found between the cost of transports (P = 0.67) of the sham-injected and BoNT/E groups. There was a difference in the cost of transport as it relates to the speeds tested (P < 0.0001), cost of transports being highest at low speeds and decreasing as speed increased. These results indicate that botulinum E interferes with the swimming speed of the catfish, which could contribute to the mortality from the disease of VTC and potentially make the fish more susceptible to predation. Received September 20, 2013; accepted

  9. Relationships Among Feed Efficiency, Body Composition, and Growth in Channel Catfish (Ictalurus punctatus) Families

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Feed is a major cost in catfish production and improving feed efficiency could improve production efficiency and profitability. Improving catfish feed efficiency through genetic selection is challenging because measuring feed intake on individuals is difficult and expensive. Examining relationship...

  10. Water quality and plankton communities in hybrid catfish (female channel catfish, Ictalurus punctatus x male blue catfish, I. furcatus) ponds after partial fish harvest

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Twelve, 0.4-ha ponds were stocked with 10,000 hybrid catfish fingerlings in March 2015. Six ponds were partially harvested in August to remove fish larger than ~ 0.57 kg. All remaining fish were removed in October and November. Partial harvest of faster-growing fish removed ~26% of fish initially st...

  11. Dieldrin in the diet of channel catfish (Ictalurus punctatus): uptake and effect on growth

    USGS Publications Warehouse

    Argyle, Ray L.; Williams, George C.; Daniel, Clara B.

    1975-01-01

    Fingerling channel catfish (Ictalurus punctatus) were given diets containing 0, 0.4, 0.8, or 4.0 I?g dieldrin/g of food (dry weight) for 210 days, followed by a dieldrin-free diet for 56 days. Catfish receiving 4.0 I?g dieldrin/g food gained about 17% less weight than the control fish in 210 days and about 35% less weight during the last 126 days of the 210-day period. No growth differences were attributable to the ingestion of 0.4 or 0.8 I?g dieldrin/g of feed. Mortality during the experiment was random and not appreciable. Dieldrin accumulations were characterized by an immediate rapid rise of residue levels in the tissues, followed by a period during which concentrations were relatively stable. Residues in tissues decreased rapidly after dieldrin was withdrawn from the diet. Dieldrin half-life was 9.6 days (mean for the three groups). Theoretical residue concentrations calculated from the growth, feeding, and half-life data were similar to the empirical concentrations, although the theoretical values were consistently the higher. The recoverable concentration accounted for 25.7% (mean of the three treatments) of the theoretical concentration.

  12. Detection and quantification of virulent Aeromonas hydrophila in channel catfish tissues following waterborne challenge.

    PubMed

    Zhang, Dunhua; Moreira, Gabriel S A; Shoemaker, Craig; Newton, Joseph C; Xu, De-Hai

    2016-05-01

    The aim of this study was to understand the pathogenesis of motile aeromonas septicemia caused by an emergent, high virulent Aeromonas hydrophila (vAh) in channel catfish, Ictalurus punctatus Adipose fin clipped catfish were challenged with vAh using a waterborne challenge method, and the distribution of vAh over a time course was detected and quantified using real-time polymerase chain reaction. The results showed that 77.8% of fish died within 48 h post challenge with mean day to death of 1.5 days. At 2 h post challenge, vAh (inferred from genomic DNA copies or genome equivalents) was detected in all external and internal tissues sampled. Gill had the highest vAh cells at 1 h post challenge. Spleen harbored the most vAh cells among internal organs at 4 h post challenge. The tissues/organs with most vAh cells detected at 8 h post challenge were adipose fin, blood, intestine, kidney and skin, while liver showed the highest vAh cells at 24 h post challenge. These results suggest that vAh was able to rapidly proliferate and spread, following wound infection, through the fish blood circulation system and cause mortality within 8-24 h. PMID:27044300

  13. Chemical and visual control of feeding and escape behaviors in the channel catfish Ictalurus punctatus.

    PubMed

    Valentincic, T B; Caprio, J

    1994-05-01

    Channel catfish, maintained individually in aquaria with dark substrate, responded to visual stimuli from above with escape behavior and to amino acid stimuli with feeding behavior. Feeding behavior was composed of a) appetitive patterns, such as barbel movements, orienting posture, and search swimming and b) consummatory patterns that included a halt in swimming, turning, snapping-biting, ingestion, mastication, and swallowing. The conditioning procedure, which consisted of 90 s presentations of a single amino acid followed by a food reward, influenced the duration and speed of the search swim. Swimming behavior was quantified by counting the number of turns greater than 90 degrees. Catfish turned 40-75 times to the conditioned stimuli, L-proline and L-arginine, but only 20-40 times to the nonconditioned stimuli. Olfaction rather than taste was involved in the conditioned response to L-proline because the highest possible contact concentration (3 x 10(-6) M) of L-proline within the stimulus eddies was at least 30 times lower than the estimated L-proline electrophysiological taste threshold (> 10(-4) M). PMID:8022904

  14. In vivo responses of single olfactory receptor neurons in the channel catfish, Ictalurus punctatus.

    PubMed

    Kang, J; Caprio, J

    1995-01-01

    1. We report for the first time in any teleost, a quantitative in vivo study of recordings from single olfactory receptor neurons (ORNs) in the channel catfish, Ictalurus punctatus, with odorant stimuli. 2. Responses of 69 spontaneously active single ORNs were recorded simultaneously with the electroolfactogram (EOG). Recording times ranged from 10 to 72 min per receptor cell with an average of 24 +/- 15 (SD) min/cell. The averaged spontaneous frequency ranged from < 1 to 12 action potentials/s with a mean frequency of 4.7 +/- 2.5 action potentials/s. 3. Catfish ORNs responded to the odorant stimuli (amino acids, bile salts, and ATP) with either an excitation or suppression of the background neural activity. Suppressive responses were encountered more frequently than excitatory responses, suggesting that suppressive responses also play an important role in olfactory coding. 4. Excitatory and suppressive responses to the different odorants were elicited from the same ORN, suggesting that different olfactory receptor molecules and different transduction pathways exist in the same ORN. PMID:7714562

  15. Hepatic monooxygenase induction and promutagen activation in channel catfish from a contaminated river basin

    SciTech Connect

    Winston, G.W.; Shane, B.S.; Henry, C.B.

    1988-12-01

    To better understand the etiology of cancer in fish from polluted waters, the impact of environmental contaminants on xenobiotic metabolism of channel catfish (Ictalurus punctatus) from a highly polluted water body, Devil's Swamp in southeastern Louisiana, has been investigated. Fish from Devil's Swamp bioaccumulated polynuclear aromatic hydrocarbons (PAH), chlorinated hydrocarbon insecticides (CHI), and polychlorinated biphenyls (PCB) in fat tissue, the latter exceeding 7000 ppb. Reference catfish from the University farm, Ben Hur, were virtually devoid of PAH, CHI, and PCB. Liver microsomal enzymes (MFO) from Devil's Swamp fish were markedly induced. The specific content of cytochromes P450 and b5 and the specific activities of NAD(P)H-cytochrome c reductase were two to three times higher than those of Ben Hur fish. Consistent with this induction, a 9000g supernatant from Devil's Swamp but not Ben Hur fish activated 2-aminofluorene and benzo(a)pyrene (BP) to mutagens in the Ames test. BP metabolism by Devil's Swamp fish liver microsomes was inhibited to a greater extent by alpha-naphthoflavone than was BP metabolism by Ben Hur fish microsomes. This finding indicates that the induced activity in the Devil's Swamp fish liver was the result of P450 isozymes characteristic of PAH/PCB induction. Thus, exposure of fish to environmental pollutants can alter MFO leading to enhanced metabolic activation of promutagens to mutagens.

  16. A Leukocyte Immune-Type Receptor Subset Is a Marker of Antiviral Cytotoxic Cells in Channel Catfish, Ictalurus punctatus.

    PubMed

    Taylor, Erin B; Moulana, Mohadetheh; Stuge, Tor B; Quiniou, Sylvie M A; Bengten, Eva; Wilson, Melanie

    2016-03-15

    Channel catfish, Ictalurus punctatus, leukocyte immune type receptors (LITRs) represent a multigene family that encodes Ig superfamily proteins that mediate activating or inhibitory signaling. In this study, we demonstrate the use of mAb CC41 to monitor viral cytotoxic responses in catfish and determine that CC41 binds to a subset of LITRs on the surface of catfish clonal CTLs. Homozygous gynogenetic catfish were immunized with channel catfish virus (CCV)-infected MHC-matched clonal T cells (G14D-CCV), and PBL were collected at various times after immunization for flow cytometric analyses. The percentage of CC41(+) cells was significantly increased 5 d after primary immunization with G14D-CCV and at 3 d after a booster immunization as compared with control fish only injected with G14D. Moreover, CC41(+) cells magnetically isolated from the PBL specifically killed CCV-infected targets as measured by (51)Cr release assays and expressed messages for CD3γδ, perforin, and at least one of the CD4-like receptors as analyzed by RNA flow cytometry. When MLC effector cells derived from a G14D-CCV-immunized fish were preincubated with CC41 mAb, killing of G14D-CCV targets was reduced by ∼40%, suggesting that at least some LITRs have a role in target cell recognition and/or cytotoxicity. The availability of a LITR-specific mAb has allowed, to our knowledge for the first time, functional characterization of LITRs in an autologous system. In addition, the identification of an LITR subset as a cytotoxic cell marker will allow for more effective monitoring of catfish immune responses to pathogens. PMID:26856701

  17. Hybrid catfish offers performance advantages for US catfish farmers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel x blue hybrid catfish is the only hybrid among 28 interspecific hybrid crosses and backcrosses evaluated within the family Ictaluridae to exhibit dominant traits desirable for intensive aquaculture. In a 2004 pond trial, hybrid catfish outperformed channel catfish in all the production trai...

  18. Using 1-D 1H and 2-D 1H J-resolved NMR metabolomics to understand the effects of anemia in channel catfish (Ictalurus punctatus)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Anemia is a widespread hematological disorder in vertebrates. In channel catfish (Ictalurus punctatus), anemia is a persistent problem in culture environments, however, its causes and physiological impacts are not well understood. To better understand the symptoms and characterize associated biomark...

  19. Using 1-D and 2-D J-resolved 1H-NMR metabolomics to understand the effects of anemia in channel catfish Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Anemia is a widespread hematological disorder in vertebrates. In channel catfish (Ictalurus punctatus), anemia is a persistent problem in culture environments, however, its causes and physiological impacts are not well understood. To better understand the symptoms and characterize associated biomark...

  20. In vitro and in vivo evaluation of potassium permanganate treatment efficacy for the control of acute experimental infection of flavobacterium columnare in channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An experimental trial was performed to evaluate the efficacy of potassium permanganate against an acute and systemic experimental infection of Flavobacterium columnare in channel catfish, Ictalurus punctatus. The infection was produced by waterborne exposure to the bacteria after mechanical cutaneo...

  1. Plasma corticosteroid dynamics in channel catfish, Ictalurus punctatus (Rafinesque), during and after oxygen depletion

    USGS Publications Warehouse

    Tomasso J.R., Davis, K. B.; Parker, N.C.

    1981-01-01

    Plasma corticosteroid concentrations in channel catfish, Ictalurus punctatus, (normally 1.0 ± 0.3 μg/100 ml) increased significantly (to 5.9 ± 1.2μg/100 ml) in response to acute oxygen depletion and then returned to control levels within 30 min after the dissolved oxygen concentration was increased; however, a secondary increase in plasma corticosteroid levels was observed 6 h after exposure. Corticosteroid levels also increased in fish exposed to dissolved oxygen concentration of <0.2 mg/1 for three days. Methylene blue was not effective in preventing interrenal response to low dissolved oxygen. No diurnal plasma corticosteroid rhythm was observed in fish exposed to diurnal chemical rhythms of culture ponds.

  2. Liquid-chromatographic determination of sarafloxacin residues in channel catfish muscle-tissue

    USGS Publications Warehouse

    Meinertz, J.R.; Dawson, V.K.; Gingerich, W.H.; Cheng, B.; Tubergen, M.M.

    1994-01-01

    A liquid chromatographic method is described for the determination of sarafloxacin hydrochloride residues i n channel catfish (ictalurus punctatus) fillets. Sarafloxacin was extracted from fillet tissue with acetonitrile=water (1 + 1). The extract was centrifuged and the supernatant was partitioned with hexane. The aqueous fraction was filtered through a 0.45 Mum filter and evaporated to dryness. The sample was redissolved with 20% acetonitrile-methanol (3 + 2) and 80% trifluoroacetic acid (0.1%), Centrifuged, and filtered to remove proteins. Samples were analyzed by chromatography with gradient elution on a c18 column and with fluorescence detection (excitation at 280 nm and emission above 389 nm). Mean recoveries ranged from 85.4 To 104%, and relative standard deviations ranged from 1.06 To 5.58% In samples spiked at concentrations of 10.0-863.8 Ng/g. The method detection limit for sarafloxacin was 1.4 Ng/g.

  3. Production of channel catfish with sperm cryopreserved by rapid non-equilibrium cooling.

    PubMed

    Cuevas-Uribe, Rafael; Leibo, S P; Daly, Jonathan; Tiersch, Terrence R

    2011-12-01

    This report describes the feasibility of using vitrification for fish sperm. Vitrification can be used to preserve samples in the field and offers an alternative to conventional cryopreservation, although it has not been systematically studied for sperm of aquatic species. The overall goal of the project was to develop streamlined protocols that could be integrated into a standardized approach for vitrification of aquatic species germplasm. The objectives of the present study in channel catfish (Ictalurus punctatus) were to: (1) evaluate the acute toxicity of 5%, 10%, 20% and 30% methanol, N,N-dimethyl acetamide, dimethyl sulfoxide, 1,2-propanediol, and methyl glycol; (2) evaluate a range of devices commonly used for cryopreservation and vitrification of mammalian sperm; (3) compare vitrification with and without cryoprotectants; (4) evaluate the post-thaw membrane integrity of sperm vitrified in different cryoprotectant solutions, and (5) evaluate the ability of vitrified sperm to fertilize eggs. Cryoprotectant concentrations of higher than 20% were found to be toxic to sperm. Methanol and methyl glycol were the least toxic at a concentration of 20% with an exposure time of less than 5 min. We evaluated a method reported for human sperm, using small volumes in loops (15 μl) or cut standard straws (20 μl) with and without cryoprotectants plunged into liquid nitrogen. Cryoprotectant-free vitrification using loops did not yield fertilization (assessed by neurulation), and the fertilization rates observed in two trials using the cut standard straws were low (~2%). In general, fertilization values for vitrification experiments were low and the use of low concentrations of cryoprotectants yielded lower fertilization (<10%) than the use of vitrification solutions containing high cryoprotectant concentrations (as high as 25%). The highest neurulation obtained was from a mixture of three cryoprotectants (20% methanol+10% methyl glycol+10% propanediol) with a single

  4. Genome-wide identification of Hsp70 genes in channel catfish and their regulated expression after bacterial infection.

    PubMed

    Song, Lin; Li, Chao; Xie, Yangjie; Liu, Shikai; Zhang, Jiaren; Yao, Jun; Jiang, Chen; Li, Yun; Liu, Zhanjiang

    2016-02-01

    Heat shock proteins 70/110 (Hsp70/110) are a family of conserved ubiquitously expressed heat shock proteins which are produced by cells in response to exposure to stressful conditions. Besides the chaperone and housekeeping functions, they are also known to be involved in immune response during infection. In this study, we identified 16 Hsp70/110 geness in channel catfish (Ictalurus punctatus) through in silico analysis using RNA-Seq and genome databases. Among them 12 members of Hsp70 (Hspa) family and 4 members of Hsp110 (Hsph) family were identified. Phylogenetic and syntenic analyses provided strong evidence in supporting the orthologies of these HSPs. In addition, we also determined the expression patterns of Hsp70/110 genes after Flavobacterium columnare and Edwardsiella ictaluri infections by meta-analyses, for the first time in channel catfish. Ten out of sixteen genes were significantly up/down-regulated after bacterial challenges. Specifically, nine genes were found significantly expressed in gill after F. columnare infection. Two genes were found significantly expressed in intestine after E. ictaluri infection. Pathogen-specific pattern and tissue-specific pattern were found in the two infections. The significantly regulated expressions of catfish Hsp70 genes after bacterial infections suggested their involvement in immune response in catfish. PMID:26693666

  5. Molecular immune response of channel catfish immunized with live theronts of Ichthyophthirius multifiliis.

    PubMed

    Xu, De-Hai; Zhang, Qi-Zhong; Shoemaker, Craig A; Zhang, Dunhua; Moreira, Gabriel S A

    2016-07-01

    The parasite Ichthyophthirius multifiliis (Ich) has been reported in various freshwater fishes worldwide and results in severe losses to both food and aquarium fish production. The fish surviving natural infections or immunized with live theronts develop strong specific and non-specific immune responses. Little is known about how these immune genes are induced or how they interact and lead to specific immunity against Ichthyophthirius multifiliis in channel catfish Ictalurus punctatus. This study evaluated the differential expression of immune-related genes, including immunoglobulin, immune cell receptor, cytokine, complement factor and toll-like receptors in head kidney from channel catfish at different time points after immunization with live theronts of I. multifiliis. The immunized fish showed significantly higher anti-Ich antibody expressed as immobilization titer and ELISA titer than those of control fish. The vast majority of immunized fish (95%) survived theront challenge. Expression of IgM and IgD heavy chain genes exhibited a rapid increase from 4 hour (h4) to 2 days (d2) post immunization. Expression of immune cell receptor genes (CD4, CD8-α, MHC I, MHC II β, TcR-α, and TcR-β) showed up-regulation from h4 to d6 post immunization, indicating that different immune cells were actively involved in cellular immune response. Cytokine gene expression (IL-1βa, IL-1βb, IFN-γ and TNF-α) increased rapidly at h4 post immunization and were at an up-regulated level until d2 compared to the bovine serum albumin control. Expression of complement factor and toll-like receptor genes exhibited a rapid increase from h4 to d2 post immunization. Results of this study demonstrated differential expression of genes involved in the specific or non-specific immune response post immunization and that the vaccination against Ich resulted in protection against infection by I. multifiliis. PMID:27044331

  6. Bioconcentration and endocrine disruption effects of diazepam in channel catfish, Ictalurus punctatus.

    PubMed

    Overturf, C L; Overturf, M D; Huggett, D B

    2016-01-01

    Recently, the detection of pharmaceuticals in surface waters has increased worldwide. Pharmaceuticals are typically found in the environment at concentrations well below therapeutic levels in humans; however, their mechanisms of action may be largely unknown in non-target organisms, such as teleost species. Thus, chronic exposure to these types of compounds warrants further investigation. The goal of this study was to examine the potential for diazepam, a model benzodiazepine drug, to bioconcentrate in tissues of channel catfish and to examine its ability to interact with the endocrine system through modulation of steroid hormones and/or steroidogenic genes. To investigate the bioconcentration potential of diazepam, channel catfish (Ictalurus punctatus) were exposed to 1ng/mL diazepam for seven days, followed by clean water for another seven days, using an abbreviated OECD 305 Fish Bioconcentration Test study design. This concentration of diazepam is well below environmentally relevant concentrations of diazepam (ng/L). To evaluate steroidogenic effects, fish were exposed to 1ng/mL diazepam for seven days only. Steroid hormone concentrations were analyzed for various tissues, as well as expression of selected steroidogenic genes. Calculated bioconcentration factors for diazepam were well below regulatory threshold values in all tissues analyzed. No changes in steroid hormone concentration were detected in any tissue analyzed; however, the steroidogenic gene cytochrome P450 side chain cleavage (P450scc) was significantly down-regulated at day 5 and 3β-hydroxy steroid dehydrogenase (3β-HSD) was significantly down-regulated at day 7 in the gonad. These results indicate that although diazepam does not significantly bioconcentrate, low-level chronic exposure to diazepam may have the potential to interact with endocrine function by altering gene expression. PMID:26875913

  7. Pharmacokinetics of Intravascularly Administered (65)Zinc in Channel Catfish (Ictalurus punctatus)

    SciTech Connect

    Barron, Mace G.; Schultz, Irvin R. ); Newman, Michael E.

    1999-12-01

    We compared the pharmacokinetics of the radioisotope 65Zinc (65Zn) in blood, plasma, and whole body of adult channel catfish (Ictalurus punctatus) following intravascular (iv) administration. A two compartment model described the pharmacokinetics of 65Zn in plasma and blood during the first 40 days following iv administration, but was unable to describe the long-term disposition of 65Zn. Whole body counting revealed that approximately half of the 65Zn dose was sequestered in a slowly exchangeable pool with a half-life of 1.5 years. Greater than 99% of the circulating 65Zn was bound to plasma proteins, whereas there was less than 1% binding to red blood cells. Synthesis of the results for channel catfish and existing data in other species indicates three phases in the pharmacokinetics of zinc. The first phase consists of initial distribution outside the vascular system to kidney, liver, and other organs (alpha phase in blood and plasma; t1/2 of 4 to 5 hours). The second phase involves distribution from organs to a slowly exchangeable zinc pool, likely consisting of bone (beta phase in blood and plasma; alpha phase in whole body; t1/2 of 4 to 20 days). The third phase appears to involve a slow turnover of sequestered zinc (t1/2 greater than 1 year). Blood sampling or short-term whole body measurements will underestimate the persistence of zinc in fish, thus prolonged sampling and measurement of whole body concentrations are necessary to characterize the pharmacokinetics of zinc.

  8. The cytochrome P450 genes of channel catfish: their involvement in disease defense responses as revealed by meta-analysis of RNA-Seq datasets

    PubMed Central

    Zhang, Jiaren; Yao, Jun; Wang, Ruijia; Zhang, Yu; Liu, Shikai; Sun, Luyang; Jiang, Yanliang; Feng, Jianbin; Liu, Nannan; Nelson, David; Waldbieser, Geoff; Liu, Zhanjiang

    2015-01-01

    Background Cytochrome P450s (CYPs) encode one of the most diverse enzyme superfamily in nature. They catalyze oxidative reactions of endogenous molecules and exogenous chemicals. Methods We identified CYPs genes through in silico analysis using EST, RNA-Seq and genome databases of channel catfish. Phylogenetic analyses and conserved syntenic analyses were conducted to determine their identities and orthologies. Meta-analysis of RNA-Seq databases was conducted to analyze expression profile of CYP genes following bacterial infection. Results A full set of 61 CYP genes were identified and characterized in channel catfish. Phylogenetic tree and conserved synteny provided strong evidence of their identities and orthorlogy. Lineage-specific gene duplication was evident in a number of clans in channel catfish. CYP46A1 is missing in the catfish genome as observed with syntenic analysis and RT-PCR analysis. Thirty CYPs were found up- or down-regulated in liver, while seven and eight CYPs were observed regulated in intestine and gill following bacterial infection. Conclusion We systematically identified and characterized a full set of 61 CYP genes in channel catfish and studied their expression profiles after bacterial infection. Strikingly large numbers of CYP genes appear to be involved in the bacterial defense processes. General significance This work provides an example to systematically study CYP genes in non-model species. Moreover, it provides a basis for further toxicological and physiological studies in channel catfish. PMID:24780645

  9. Electrophysiological evidence for the broad distribution of specific odorant receptor molecules across the olfactory organ of the channel catfish.

    PubMed

    Chang, Q; Caprio, J

    1996-10-01

    To determine if there is a spatial segregation of responsiveness to odorants within the olfactory epithelium, microelectrode recordings were obtained from small populations of olfactory receptor neurons located across different lamellar sensory regions of the olfactory organ of the channel catfish, lctalurus punctatus. Stimuli included L-alanine, L-methionine, L-arginine hydrochloride, L-glutamic acid, ATP and a mixture of bile salts-odorants previously reported to stimulate independent receptor sites in aquatic species. The peak integrated olfactory receptor responses at each recording site were standardized to the response to L-alanine. The relative stimulatory effectiveness of the stimuli was preserved across the 10 olfactory lamellae recording sites. These data support previous molecular biological results of a broad distribution of receptor neurons that express specific receptor genes across the olfactory organ of the channel catfish. PMID:8902281

  10. Channel catfish (Ictalurus punctatus) leukocytes express estrogen receptor isoforms ERα and ERβ2 and are functionally modulated by estrogens

    USGS Publications Warehouse

    Iwanowicz, Luke R.; Stafford, James L.; Patiño, Reynaldo; Bengten, Eva; Miller, Norman W.; Blazer, Vicki

    2014-01-01

    Estrogens are recognized as modulators of immune responses in mammals and teleosts. While it is known that the effects of estrogens are mediated via leukocyte-specific estrogen receptors (ERs) in humans and mice, leucocyte-specific estrogen receptor expression and the effects of estrogens on this cell population is less explored and poorly understood in teleosts. Here in, we verify that channel catfish (Ictalurus punctaus) leukocytes express ERα and ERβ2. Transcripts of these isoforms were detected in tissue-associated leukocyte populations by PCR, but ERβ2 was rarely detected in PBLs. Expression of these receptors was temporally regulated in PBLs following polyclonal activation by concanavalin A, lipopolysaccharide or alloantigen based on evaluation by quantitative and end-point PCR. Examination of long-term leukocyte cell lines demonstrated that these receptors are differentially expressed depending on leukocyte lineage and phenotype. Expression of ERs was also temporally dynamic in some leukocyte lineages and may reflect stage of cell maturity. Estrogens affect the responsiveness of channel catfish peripheral blood leukocytes (PBLs) to mitogens in vitro. Similarly, bactericidal activity and phorbol 12-myristate 13-acetate induced respiratory burst was modulated by 17β-estradiol. These actions were blocked by the pure ER antagonist ICI 182780 indicating that response is, in part, mediated via ERα. In summary, estrogen receptors are expressed in channel catfish leukocytes and participate in the regulation of the immune response. This is the first time leukocyte lineage expression has been reported in teleost cell lines.

  11. Safety of aquaflor (florfenicol, 50% type a medicated article), administered in feed to channel catfish, Ictalurus punctatus

    USGS Publications Warehouse

    Gaikowski, Mark P.; Wolf, Jeffery C.; Endris, Richard G.; Gingerich, William H.

    2003-01-01

    Aquaflor, a feed premix containing the broad spectrum antibacterial agent florfenicol (50% w/w), is being developed for use to control enteric septicemia (ESC) in channel catfish Ictalurus punctatus caused by the gram-negative enterobacterium Edwardsiella ictaluri. The recommended dose of Aquaflor to control ESC is 10 mg/kg body weight (BW)/day for 10 days. The study objective was to determine the safety of Aquaflor administered in feed to channel catfish at doses of 0 (control), 10, 30, and 50 mg/kg BW/day for 20 consecutive days. Parameters evaluated included daily mortality, behavioral (appetite, distribution, flight/fright response), and water chemistry observations, initial and terminal weight measurements, and gross and microscopic pathology. Medicated feed consumption was 67-86% of target with group mean doses of 8.5 mg/kg BW/day, 24.6 mg/kg BW/day, and 34.9 mg/kg BW/day. There were no mortalities or clinically observable changes noted at any of the dose levels tested. Aquaflor-related changes were limited to the food consumption and histopathology data. Although Aquaflor-related decreased feed consumption was noted in the 30 and 50 mg/kg BW/day groups, there were no differences in fish growth among the treatment groups. Aquaflor-related histopathology findings were limited to a histomorphologically evident dose-dependent decrease in hematopoietic/lymphopoietic tissue in the anterior kidneys, posterior kidneys, and spleens of channel catfish.

  12. Safety of Aquaflor (Florfenicol, 50% Type A Medicated Article), Administered in Feed to Channel Catfish, Ictalurus punctatus

    USGS Publications Warehouse

    Gaikowski, M.P.; Wolf, J.C.; Endris, R.G.; Gingerich, W.H.

    2003-01-01

    Aquaflor, a feed premix containing the broad spectrum antibacterial agent florfenicol (50% w/w), is being developed for use to control enteric septicemia (ESC) in channel catfish Ictalurus punctatus caused by the gram-negative enterobacterium Edwardsiella ictaluri. The recommended dose of Aquaflor to control ESC is 10 mg/kg body weight (BW)/day for 10 days. The study objective was to determine the safety of Aquaflor administered in feed to channel catfish at doses of 0 (control), 10, 30, and 50 mg/kg BW/day for 20 consecutive days. Parameters evaluated included daily mortality, behavioral (appetite, distribution, flight/fright response), and water chemistry observations, initial and terminal weight measurements, and gross and microscopic pathology. Medicated feed consumption was 67-86% of target with group mean doses of 8.5 mg/kg BW/day, 24.6 mg/kg BW/day, and 34.9 mg/kg BW/day. There were no mortalities or clinically observable changes noted at any of the dose levels tested. Aquaflor-related changes were limited to the food consumption and histopathology data. Although Aquaflor-related decreased feed consumption was noted in the 30 and 50 mg/kg BW/day groups, there were no differences in fish growth among the treatment groups. Aquaflor-related histopathology findings were limited to a histomorphologically evident dose-dependent decrease in hematopoietic/lymphopoietic tissue in the anterior kidneys, posterior kidneys, and spleens of channel catfish.

  13. Reducing size variation in hybrid catfish culture through graded partial harvest

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Hybrid Catfish (' Channel Catfish Ictalurus punctatus x ' Blue Catfish I. furcatus) have quickly become a major contributor to ictalurid catfish aquaculture in the United States. Hybrid Catfish have unique production characteristics compared with Channel Catfish, and rapid farmer adoption of the hyb...

  14. Textural and Compositional Comparison of Channel and Hybrid Catfish from Pond and Raceway Environments

    Technology Transfer Automated Retrieval System (TEKTRAN)

    ince the peak of the US catfish industry in 2003, the amount of catfish sold to processors has decreased by more than half, from 662 million pounds to 300 million pounds in 2012. This has increased the importance of producing a product with consistently high quality in an efficient manner, which ha...

  15. Statistical approaches to optimize detection of MIB off-flavor in aquaculture raised channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The catfish industry prides itself on preventing inadvertent sale of off-flavor fish. Typically, several fish are taste tested over several weeks before pond harvest to confirm good fish flavor quality. We collected several data sets of analytically measured off-flavor concentrations in catfish to...

  16. Economic analysis and treatment of trematode infections in channel catfish production ponds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bolbophorus spp. have been associated with high mortalities and productions loss in commercial catfish. To assess the economic impact, a study was conducted on a commercial catfish operation with trematode infections. Fish were sampled (n=40 ponds) and examined for metacercariae. Each pond was pla...

  17. Effects of Bio-Mos on Growth and Survival of Channel Catfish Challenged with Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A major problem in the catfish farming industry has been high disease loss to enteric septicemia of catfish (ESC), caused by the bacterium Edwardsiella ictaluri (E. ictaluri). Methods to control this disease include antibiotic therapy, vaccinations, and management strategies such as taking the fish...

  18. Experimental induction of motile Aeromonas septicemia in channel catfish by waterborne challenge with virulent Aeromonas hydrophila

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Motile Aeromonas septicemia (MAS), caused by virulent clonal isolates of Aeromonas hydrophila (vAh), is emerging as a major disease in catfish aquaculture in the Southeastern United States. Predisposing conditions leading to vAh infection in catfish were however largely unknown. The objective of thi...

  19. Reduction of Listeria monocytogenes on the surface of fresh channel catfish fillets by bacteriophage Listex P100.

    PubMed

    Soni, Kamlesh A; Nannapaneni, Ramakrishna; Hagens, Steven

    2010-04-01

    Bacteriophage Listex P100 (phage P100) was approved by the U.S. Food and Drug Administration and U.S. Department of Agriculture's Food Safety and Inspection Service for Listeria monocytogenes control on both raw and ready-to-eat food products. In this article, we present the proof of concept on the influence of phage dose, phage contact time, and storage temperature on the listericidal activity of phage P100 in reducing the L. monocytogenes loads on the surface of fresh channel catfish fillet. The fresh catfish fillet samples were surface inoculated with approximately 4.3 log(10) colony forming units (CFU)/g of a two serotype mix (1/2a and 4b) of L. monocytogenes cells and then surface treated with phage P100. L. monocytogenes reduction was influenced by phage contact time and phage dose regardless of higher or lower temperature regimes tested on catfish fillet. The reduction in L. monocytogenes loads (p < 0.05) with the phage P100 dose of 2 x 10(7) plaque forming units (PFU)/g (7.3 log(10) PFU/g) was 1.4-2.0 log(10) CFU/g at 4 degrees C, 1.7-2.1 log(10) CFU/g at 10 degrees C, and 1.6-2.3 log(10) CFU/g at room temperature (22 degrees C) on raw catfish fillet. The phage contact time of 30 min was adequate to yield greater than 1 log(10) CFU/g reduction in L. monocytogenes, whereas 15 min contact time with phage yielded less than 1 log(10) CFU/g reduction in L. monocytogenes loads on catfish fillet. Phage P100 titer was stable on catfish fillet samples, and overall reductions in L. monocytogenes counts were still maintained over a 10-day shelf life at 4 degrees C or 10 degrees C by phage P100 treatment. These findings illustrate the effectiveness of an alternative generally recognized as safe antimicrobial such as bacteriophage Listex P100 in quantitatively reducing L. monocytogenes from fresh catfish fillet surfaces. PMID:19958102

  20. Chloride inhibition of nitrite-induced methemoglobinemia in channel catfish (Ictalurus punctatus)

    USGS Publications Warehouse

    Tomasso, J.R.; Simco, B.A.; Davis, K.B.

    1979-01-01

    Exposure of channel catfish (Ictalurus punctatus) fingerlings for 24?h to 1.0, 2.5, and 5.0?mg/L nitrite (pH?=?7; hardness?=?40?mg/L; temperature?=?22–25 °C) produced methemoglobin levels of 20.7?±?1.9%, 59.8?±?1.9%, and 77.4?±?1.4% (SE), respectively. However, methemoglobin levels were not elevated when fish were simultaneously exposed to 1.0, 2.5, and 5.0?mg/L nitrite and 25, 50, and 100?mg/L sodium chloride, respectively. Acclimation to sodium chloride for 24?h before exposure to nitrite did not enhance the inhibitory action of sodium chloride. Fish exposed to 5?mg/L nitrite for 5?h developed 42.5?±?3.8% methemoglobin. When transferred to water containing 5?mg/L nitrite and 250?mg/L sodium chloride, methemoglobin levels returned to normal within 24?h. Environmental chloride probably inhibits methemoglobin formation by competing with nitrite for entrance into the gills of the fish. An ionic ratio of 16 Cl- to 1 NO2- is capable of complete suppression of nitrite-induced methemoglobin formation. Bicarbonate ion present in the test water (1?meq/L) may also have contributed to the inhibitive action of chloride.

  1. Consummatory feeding behavior to amino acids in intact and anosmic channel catfish Ictalurus punctatus.

    PubMed

    Valentincic, T; Caprio, J

    1994-05-01

    The entire sequence of feeding behavior patterns exhibited by intact and anosmic channel catfish to food extracts was also released by single amino acids. L-arginine (> 10(-6) M), L-alanine (> 10(-6) M), and L-proline (> 10(-4) M) were each highly effective at releasing consummatory behavior patterns, such as turning, increasing pumping of water across the gill arches, and biting-snapping. Swallowing required solid objects, whereas rhythmic movement of the hyoid was released by > 10(-2) M L-arginine alone. For the biting-snapping behavior, the number of bites depended upon both the number of eddies containing the amino acid above the behavioral threshold concentration and the amino acid applied. Multiple eddies of > 10(-3) M L-proline and L-alanine provoked up to 25 bites per test; however, the most effective stimulus for releasing biting-snapping behavior at low concentrations was L-arginine (behavioral threshold 3 x 10(-7) M). In comparison to 10(-4) M L-alanine and L-arginine, other amino acids were less effective stimuli. PMID:8022905

  2. Pharmacokinetics, tissue distribution, and metabolism of nitrofurantoin in the channel catfish (Ictalurus punctatus)

    USGS Publications Warehouse

    Stehly, G.R.; Plakas, S.M.

    1993-01-01

    The pharmacokinetics, tissue distribution, and metabolism of the drug nitrofurantoin were examined in the channel catfish (Ictalurus punctatus) after intravascular or oral dosing. Mean plasma concentrations of nitrofurantoin after intravascular administration at 1 and 10 mg/kg of body weight were best fit to two- and three-compartment pharmacokinetic models, respectively. Nitrofurantoin was rapidly eliminated from the plasma after intravascular dosing; at 1 and 10 mg/kg, the terminal half-lives were 23 and 46 min, respectively. After oral dosing at 1 mg/kg, peak plasma concentrations (0.06 mu g/ml) occurred at 2 h; the bioavailability was 17%. Residues of nitrofurantoin and its metabolites in the tissues were initially eliminated rapidly but persisted at the later sampling times. Residue concentrations were highest in the plasma and excretory tissues. Approximately 21% and 4% of the oral dose were eliminated in the urine and bile, respectively. Parent nitrofurantoin was the major radiolabelled compound found in the urine; however, the percentage of total residues composed of metabolites increased with time. Biliary residues consisted mostly of nitrofurantoin metabolites. High-performance liquid chromatography revealed the presence of at least five metabolites in the urine and bile.

  3. Metabolic responses of channel catfish (Ictalurus punctatus) exposed to phenol and post-exposure recovery.

    PubMed

    Moraes, Fernanda D; Rossi, Priscila A; Figueiredo, Juliana S L; Venturini, Francine P; Cortella, Lucas R X; Moraes, Gilberto

    2016-05-31

    Metabolic adjustments were studied in channel catfish Ictalurus punctatus exposed to 1.5 mg L-1 of phe nol (10% LC50) for four days and recovered for seven days. Lower triacylglycerol (TGA) stores and increased muscle fat free acids (FFA) suggest fat catabolism in muscle. Remarkable liver FFA decrease (-31%) suggests liver fat catabolism as well. Increased muscular ammonia levels and ASAT (aspartate aminotransferase) and decreased plasma aminoacids suggest higher muscular amino acid uptake. Constant levels of glucose and increased liver glycogen stores, associated with lower amino acids in plasma, indicate gluconeogenesis from amino acids. This is supported by higher hepatic ALAT and ASAT. Higher hepatic LDH followed by lower plasma lactate may indicate that plasma lactate was also used as gluconeogenic substrate. Biochemical alterations were exacerbated during the post-exposure recovery period. Reduction in muscle and plasma protein content indicate proteolysis. A higher rate of liver fat catabolism was resulted from a remarkable decrease in hepatic TGA (-58%). Catabolic preference for lipids was observed in order to supply such elevated energy demand. This study is the first insight about the metabolic profile of I. punctatus to cope with phenol plus its ability to recover, bringing attention to the biological consequences of environmental contamination. PMID:27254449

  4. The effects of diets containing standard soybean oil, soybean oil enhanced with conjugated linoleic acids, menhaden fish oil, or an algal docosahexaenoic acid supplement on juvenile channel catfish performance, hematology

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Current commercial diets for Channel Catfish contain little or no marine fish oil to reduce diet cost and address environmental concerns. However, there is conflicting data on the effects of fish oil and other lipid sources in juvenile Channel Catfish, and some novel lipids have not been tested agai...

  5. Elucidating the roles of gut neuropeptides on channel catfish feed intake, glycemia, and hypothalamic NPY and POMC expression.

    PubMed

    Schroeter, Julie C; Fenn, Carlin M; Small, Brian C

    2015-10-01

    Both intrinsic and extrinsic factors modulate food intake and glycemia in vertebrates, in part through interactions with hypothalamic neuropeptide Y (NPY) and proopiomelanocortin (POMC) neurons. The objective of this project was to elucidate the effects of ghrelin (GHRL), gastrin-releasing peptide (GRP), cholecystokinin (CCK), glucagon-like peptide (GLP), pancreatic polypeptide (PP), and peptide YY (PYY) on appetite, glycemia, and hypothalamic expression of NPY and POMC in channel catfish. Catfish were injected intraperitoneally with a single peptide at concentrations of either 0 (control), 50, 100, or 200 ng/g body weight (BW), respectively. Fish were allowed to recover for 30 min, and then fed to satiation over 1 h. Feed intake was determined 1h post-feeding. Catfish injected with GHRL at 50 and 100 ng/g BW and GRP at 200 ng/g BW consumed significantly (P<0.05) less feed compared to controls. A tendency (P<0.1) to suppress feed intake was also observed in the 200 ng/g BW GHRL and PP treatments. PYY, CCK, and GLP had no effects on feed intake. Glycemia was not affected by GHRL, GRP, PP, and PYY treatments, but was suppressed by CCK. A tendency toward lower plasma glucose concentrations was observed in fish administered GLP at 50 ng/g BW. Hypothalamic NPY expression was highly variable and not significantly affected by treatment. POMC expression was also variable, but tended to be reduced by the highest concentration of CCK. These results provide new insight into the roles and regulation of gut neuropeptides in catfish appetite and glycemia. PMID:26151373

  6. Histopathology of repeated, intermittent exposure of chloramine-T to walleye (Sander vitreum) and (Ictalurus punctalus) channel catfish

    USGS Publications Warehouse

    Gaikowski, M.P.; Densmore, Christine L.; Blazer, V.S.

    2009-01-01

    Chloramine-T (Cl-T) has been used safely and effectively to control bacterial gill disease in salmonids at a maximum exposure regimen of up to four consecutive, once-daily exposures administered for 60??min at 20??mg/L. However, data to document safe treatment concentrations of Cl-T are lacking for freshwater-reared fish other than salmonids. We report the histopathology resultant from the administration of 12 consecutive, once-daily, 180-min static immersion baths of 0, 20, 50, or 80??mg Cl-T/L to walleye (20????C) and channel catfish (27????C). Twelve fish of each species were euthanized immediately before the first exposure (initial controls) and then after the twelfth exposure and 7 and 14??days after the twelfth exposure. Only initial controls and fish euthanized immediately after the twelfth exposure were processed for histological review because of the general lack of exposure-related lesions in exposed fish. The only exposure-related histological changes were in the spleen where significantly greater erythrocyte swelling and necrosis was observed in channel catfish exposed at 80??mg/L relative to exposure at 0??mg/L; similar histological changes were insignificant for walleye, though there appeared to be a shift in the general category of histological change with degenerative changes (necrosis, etc.) observed following exposure at 50 or 80??mg/L compared to the inflammatory and hemodynamic changes (congestion, leukocyte infiltrate, etc.) observed in walleye exposed at 0 or 20??mg/L. The only significant change in peripheral blood cytology was that walleye fingerlings exposed at 80??mg/L had significantly fewer mature red blood cells and significantly more immature red blood cells per oil-immersion field than controls. The histopathological changes observed following exposure to Cl-T under an exaggerated exposure regimen suggest that walleye or channel catfish therapeutically exposed to Cl-T will not have treatment-related histological changes.

  7. Effects of environmental pH and calcium on ammonia toxicity in channel catfish

    USGS Publications Warehouse

    Tomasso, J.R.; Goudie, C.A.; Simco, B.A.; Davis, K.B.

    1980-01-01

    The twenty-four-hour median lethal concentrations (24-hour LC50) of total ammonia nitrogen (TA-N) to channel catfish (Ictalurus punctatus) at pH 7, 8, and 9 (total hardness, 40 mg/liter; temperature, 21–25 C) were 263.6 ± 11.3 (SE), 38.8 ± 1.8, and 4.5 ± 0.2 mg/liter, respectively. The 24-hour LC50 of un-ionized ammonia nitrogen (UIA-N) concentration at pH 8 was significantly higher (1.82 ± 0.06 mg/liter) than at pH 7 or 9 (1.39 ± 0.06 and 1.49 ± 0.12 mg/liter). Enrichment of the water to 440 mg/liter total hardness at pH 7 significantly increased the 24-hour LC50 of TA-N and UIA-N (356.3 ± 16.4 and 1.79 ± 0.07). Fish exposed to 25 mg/liter TA-N for 12 hours at pH 7 and 8 showed no differences from control fish in hematocrit, percent total plasma protein, or plasma and muscle chloride. Plasma sodium showed no difference between control and experimental groups at pH 7; however, a significant decrease occurred in fish exposed to 25 mg/liter TA-N at pH 8. No differences in blood pH were found between the control groups and fish exposed to 100 and 200 mg/liter TA-N at pH 7, and to 10 and 25 mg/liter TA-N at pH 8. Plasma sodium depletion is suggested as a contributing mechanism of ammonia toxicity.

  8. Cloning, in Vitro expression, and novel phylogenetic classification of a channel catfish estrogen receptor

    USGS Publications Warehouse

    Xia, Z.; Patino, R.; Gale, W.L.; Maule, A.G.; Densmore, L.D.

    1999-01-01

    We obtained two channel catfish estrogen receptor (ccER) cDNA from liver of female fish using RT–PCR. The two fragments were identical in sequence except that the smaller one had an out-of-frame deletion in the E domain, suggesting the existence of ccER splice variants. The larger fragment was used to screen a cDNA library from liver of a prepubescent female. A cDNA was obtained that encoded a 581-amino-acid ER with a deduced molecular weight of 63.8 kDa. Extracts of COS-7 cells transfected with ccER cDNA bound estrogen with high affinity (Kd = 4.7 nM) and specificity. Maximum parsimony and Neighbor Joining analyses were used to generate a phylogenetic classification of ccER on the basis of 18 full-length ER sequences. The tree suggested the existence of two major ER branches. One branch contained two clearly divergent clades which included all piscine ER (except Japanese eel ER) and all tetrapod ERα, respectively. The second major branch contained the eel ER and the mammalian ERβ. The high degree of divergence between the eel ER and mammalian ERβ suggested that they also represent distinct piscine and tetrapod ER. These data suggest that ERα and ERβ are present throughout vertebrates and that these two major ER types evolved by duplication of an ancestral ER gene. Sequence alignments with other members of the nuclear hormone receptor superfamily indicated the presence of 8 amino acids in the E domain that align exclusively among ER. Four of these amino acids have not received prior research attention and their function is unknown. The novel finding of putative ER splice variants in a nonmammalian vertebrate and the novel phylogenetic classification of ER offer new perspectives in understanding the diversification and function of ER.

  9. Specific serum antibody responses in channel catfish (Ictalurus punctatus) provide limited protection against Streptococcus ictaluri challenge.

    PubMed

    Pasnik, David J; Evans, Joyce J; Klesius, Phillip H

    2011-11-15

    Passive immunization studies were conducted to determine the role of specific antibodies in immunity to Streptococcus ictaluri. Adult channel catfish (Ictalurus punctatus) were injected i.p. with tryptic soy broth as control or with 1.5 × 10(7)colony-forming units (cfu) S. ictaluri/fish at 0, 30, and 60 d, and serum was collected 90 d after the original challenge. Fish were passively immunized by i.p. injection with serum from the tryptic soy broth (TSB) control group, anti-S. ictaluri serum from fish immunized three times and sampled at 90 d (SSI), or heat-inactivated anti-S. ictaluri serum from fish immunized three times and sampled at 90 d (HISSI). These passively immunized fish were then challenged 72 h later with 1.5 × 10(8)cfu S. ictaluri/fish. Over 21 d, the mean cumulative percent survival was 43.3 (TSB), 63.3 (SSI), and 50.0 (HISSI). A significant difference in cumulative percent survival was noted between the TSB and the HISSI groups, and significant differences were noted between these groups and the SSI group. Serum obtained from immunized fish 72 h after passive immunization exhibited increased anti-S. ictaluri antibody levels. Twenty-one days after the challenge, the HISSI and SSI group antibody levels significantly increased above their corresponding pre-challenge levels. No significant (r(2)=0.0806; P<0.5985) correlation between increased pre-challenge specific serum antibody levels and survival after challenge was demonstrated when analyzing the control and passive immunization groups. The results indicate that both specific anti-S. ictaluri antibodies and non-specific immune responses are important for protection against S. ictaluri. PMID:21962634

  10. Editing of the Luteinizing Hormone Gene to Sterilize Channel Catfish, Ictalurus punctatus, Using a Modified Zinc Finger Nuclease Technology with Electroporation.

    PubMed

    Qin, Zhenkui; Li, Yun; Su, Baofeng; Cheng, Qi; Ye, Zhi; Perera, Dayan A; Fobes, Michael; Shang, Mei; Dunham, Rex A

    2016-04-01

    Channel catfish (Ictalurus punctatus) is the most important freshwater aquaculture species in the USA. Genetically enhanced fish that are sterile could both profit the catfish industry and reduce potential environmental and ecological risks. As the first step to generate sterile channel catfish, three sets of zinc finger nuclease (ZFN) plasmids targeting the luteinizing hormone (LH) gene were designed and electroporated into one-cell embryos, different concentrations were introduced, and the Cel-I assay was conducted to detect mutations. Channel catfish carrying the mutated LH gene were sterile, as confirmed by DNA sequencing and mating experiments. The overall mutation rate was 19.7 % for 66 channel catfish, and the best treatment was ZFN set 1 at the concentration 25 μg/ml. To our knowledge, this is the first instance of gene editing of fish via plasmid introduction instead of mRNA microinjection. The introduction of the ZFN plasmids may have reduced mosaicism, as mutated individuals were gene edited in every tissue evaluated. Apparently, the plasmids were eventually degraded without integration, as they were not detectable in mutated individuals using PCR. Carp pituitary extract failed to induce spawning and restoration of fertility, indicating the need for developing other hormone therapies to achieve reversal of sterility upon demand. This is the first sterilization achieved using ZFN technology in an aquaculture species and the first successful gene editing of channel catfish. Our results will help understand the roles of the LH gene, purposeful sterilization of teleost fishes, and is a step towards control of domestic, hybrid, exotic, invasive, and transgenic fishes. PMID:26846523

  11. Repeated handling compromises the immune suppression and improves the disease resistance in overwintering channel catfish (Ictalurus punctatus).

    PubMed

    Yang, Bingyuan; Wang, Cuicui; Hu, Huihua; Tu, Yongqin; Han, Dong; Zhu, Xiaoming; Jin, Junyan; Yang, Yunxia; Xie, Shouqi

    2015-11-01

    In winter, fish have suppressed immune functions and are susceptible to bacteria or virus which may lead to a high mortality. It is necessary to improve the immune response and disease resistance for overwintering fish. A recent study suggested that repeated handling increased the innate immune mechanisms and disease resistance in Senegalese sole. Therefore, the present study tested the hypothesis that appropriate repeated handling could compromise the immune depression and increase the disease resistance in channel catfish over winter. The experiment was carried out in field cages from Nov. 2012 to April 2013. Before the experiment, 35 fish with an average weight of 188.4 g were randomly assigned to one of six cages (2 m × 2 m × 2 m). Three cages were designed as the control group and did not receive any interfere. Fish in the other three cages received a weekly repeated handling of an air exposure for 5 min. Fish were not fed over winter. At the end of the trial, fish were challenged with Aeromonas hydrophila at a dose of 5.6 × 10(8) CFU ml(-1). The results showed that final body weight was not changed between groups. The spleen somatic index increased while the head kidney somatic index decreased in the unhandled fish after bacterial challenge, but these indices were not significantly changed in the repeated handled fish. Plasma cortisol levels in the control fish were induced at 6 h post challenge and then declined to the normal levels. However, plasma cortisol levels in the repeated handled fish did not show any significant change after bacteria challenge. The reduced inducement of heat shock protein 70 (HSP70) expression by repeated handling was observed in fish post bacterial challenge. After overwintering, repeated handled fish exhibited increased catalase (CAT) activities and reduced malondialdehyde (MDA) contents. Plasma total antioxidant capacity (TAOC), CAT and superoxide dismutase (SOD) activities of channel catfish were enhanced by repeated

  12. Growth and contribution of stocked channel catfish, Ictalurus punctatus (Rafinesque, 1818): the importance of measuring post-stocking performance

    USGS Publications Warehouse

    Stewart, David R.; Long, James M.

    2015-01-01

    In this study it was sought to quantify post-stocking growth, survival, and contribution of advanced size (178 mm total length [TL]) channel catfish Ictalurus punctatus fingerlings, something rarely done. Channel catfish populations were evaluated before (May 2010) and after (May to August 2011 and 2012) stocking. Relative abundance, stocking contribution, and growth were different (P < 0.05) in the two study impoundments (lakes Lone Chimney and Greenleaf, Oklahoma). For fish stocked in Lake Lone Chimney, stocking contribution was lower (3–35%), and average length and weight of stocked fish by age-2 reached 230 mm TL and 85 g, whereas the stocking contribution (84–98%) and growth in length (340 mm TL) and weight (280 g) were higher by age-2 in Lake Greenleaf. Given these unambiguous differences of post-stocking performance, benchmark metrics that represent population-level information such as relative abundance and average length and weight of the sample masked these significant differences, highlighting the importance of marking hatchery-fish and then following them through time to determine the effectiveness of stocking. These results suggest that stock enhancement programmes would benefit from studies that quantify post-stocking performance of hatchery fish.

  13. Molecular cloning, expression and the adjuvant effects of interleukin-8 of channel catfish (Ictalurus Punctatus) against Streptococcus iniae.

    PubMed

    Wang, Erlong; Wang, Jun; Long, Bo; Wang, Kaiyu; He, Yang; Yang, Qian; Chen, Defang; Geng, Yi; Huang, Xiaoli; Ouyang, Ping; Lai, Weimin

    2016-01-01

    Interleukin-8 (IL-8) as an important cytokine involving in inflammatory and immune response, has been studied as effective adjuvants for vaccines in mammals. However, there are fewer reports about the characterization and adjuvant effects of IL-8 in fish. In this study, cloning and sequence analysis of IL-8 coding region of channel catfish (Ictalurus punctatus) were conducted, mature IL-8(rtIL-8) was expressed and evaluated for its adjuvant effects on the immunoprotection of subunit vaccine encoding α-enolase (rENO) of Streptococcus iniae from several aspects in channel catfish. The results showed co-vaccination of rENO with rtIL-8 enhanced immune responses including humoral and cellular immunity, with higher relative percent survival(RPS,71.4%) compared with the moderate RPS of rENO alone(50%) against S. iniae infection at 4 week post vaccination. While rtIL-8 failed to maintain long-lasting immune protection, only with RPS of 26.67% in rENO + rtIL-8-vaccinated fish compared with that of rENO alone(20%) at 8 week, signifying that IL-8 hold promise for use as potential immunopotentiator in vaccines against bacterial infections in fish, whereas it is insufficient to extend the immunoprotection for long time, and further studies are required to understand the mechanisms of IL-8 used as an adjuvant and seek for more effective way to strengthen the adjuvanticity of IL-8. PMID:27373470

  14. Effects of free and combined chlorine and exposure duration on rainbow trout, channel catfish, and emerald shiners

    SciTech Connect

    Brooks, A.S.; Bartos, J.M.

    1984-11-01

    Laboratory bioassays to determine the acute toxicity of monochloramine, dichloramine, hypochlorous acid, and hypochlorite ion to emerald shiners Notropis atherinoides, channel catfish Ictalurus punctatus, and rainbow trout Salmo gairdneri were conducted. Four exposure regimes typical of chlorination schedules at operating steam electric power plants were used. Fish were exposed for single 15-minute, 30-minute, 120-minute, and 4 x 30-minute periods. Based on median lethal concentrations (LC50s), the nominal solution of hypochlorous acid was the most toxic, followed closely by the solution dominated by dichloramine; nominal monochloramine and hypochlorite ion solutions had a third to a quarter the toxicity of the other two. On the average, emerald shiners were 1.8 times more sensitive than channel catfish and 3.3 times more sensitive than rainbow trout to the four forms of chlorine. The fish were most tolerant of chlorine during short-duration exposures and least tolerant during the continuous 120-minute exposures. The differences in toxicity noted among the chlorine forms suggest that, when chlorination regimes and regulations are established, careful attention should be paid not only to total residual chlorine but to both the chlorine forms and fish species present and the duration of exposure. 24 references, 1 figure, 1 table.

  15. Molecular cloning, expression and the adjuvant effects of interleukin-8 of channel catfish (Ictalurus Punctatus) against Streptococcus iniae

    PubMed Central

    Wang, Erlong; Wang, Jun; Long, Bo; Wang, Kaiyu; He, Yang; Yang, Qian; Chen, Defang; Geng, Yi; Huang, Xiaoli; Ouyang, Ping; Lai, Weimin

    2016-01-01

    Interleukin-8 (IL-8) as an important cytokine involving in inflammatory and immune response, has been studied as effective adjuvants for vaccines in mammals. However, there are fewer reports about the characterization and adjuvant effects of IL-8 in fish. In this study, cloning and sequence analysis of IL-8 coding region of channel catfish (Ictalurus punctatus) were conducted, mature IL-8(rtIL-8) was expressed and evaluated for its adjuvant effects on the immunoprotection of subunit vaccine encoding α-enolase (rENO) of Streptococcus iniae from several aspects in channel catfish. The results showed co-vaccination of rENO with rtIL-8 enhanced immune responses including humoral and cellular immunity, with higher relative percent survival(RPS,71.4%) compared with the moderate RPS of rENO alone(50%) against S. iniae infection at 4 week post vaccination. While rtIL-8 failed to maintain long-lasting immune protection, only with RPS of 26.67% in rENO + rtIL-8-vaccinated fish compared with that of rENO alone(20%) at 8 week, signifying that IL-8 hold promise for use as potential immunopotentiator in vaccines against bacterial infections in fish, whereas it is insufficient to extend the immunoprotection for long time, and further studies are required to understand the mechanisms of IL-8 used as an adjuvant and seek for more effective way to strengthen the adjuvanticity of IL-8. PMID:27373470

  16. Evaluation of a gonadotropin releasing hormone analog of cGnRH II as a spawning aid for channel catfish versus analogs of mGnRH I and sGnRH III

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The effectiveness of three different analogs of gonadotropin-releasing hormone (GnRH) to induce spawning in female channel catfish, Ictalurus punctatus, was evaluated head to head in a blind study. Two of those analogs are currently used under Investigative New Animal Drug by the catfish industry, m...

  17. Overcoming inconsistencies in mortality rates during winter experimental challenges of channel catfish with Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    One of the goals of the ARS Catfish Genetics Research Unit is to incorporate disease resistance to ESC, caused by the bacterium, Edwardsiella ictaluri, into our selective breeding program. Through repeated experiments we have determined an optimal challenge dose of E. ictaluri that produces 50-70% ...

  18. Molecular responses of ceruloplasmin to Edwardsiella ictaluri infection and iron overload in channel catfish (Ictalurus punctatus)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Ceruloplasmin is a serum ferroxidase that carries more than 90% of the copper in plasma and has documented roles in iron homeostasis as well as antioxidative functions. In our previous studies, it has been shown that the ceruloplasmin gene is strongly up-regulated in catfish during challenge with Ed...

  19. Immunization with recombinant aerolysin and hemolysin protected channel catfish against virulent Aeromonas hydrophila

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Aeromonas hydrophila is emerging as one of the major concerns in catfish aquaculture in the Southeastern United States due to recent outbreaks of motile aeromonad septicemia (MAS) caused by virulent clonal isolates. There is no effective vaccine currently available for the prevention of MAS. In this...

  20. A Vertical-Lift Incubator (The "Seesaw") Designed for Channel Catfish Egg Masses

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Catfish spawns have been incubated the same way for nearly a century. Spawns are placed in baskets and agitated with rotating paddles. While this system is widely used, it has been recently determined that dissolved oxygen in the center of a spawn may be as much as 5 ppm less than air-saturated wate...

  1. Validation of a whole-body cortisol extraction procedure for channel catfish (Ictalurus punctatus) fry

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We developed and validated a whole-body cortisol extraction technique for catfish fry. Their small size (< 1 g) makes it difficult to measure cortisol, a common indicator of a stress response, using conventional assay methods. Three volume enhancement methods were tested: CAL method (zero calibrator...

  2. Effects of condtioning channel catfish Ictalurus punctatus to acute low water stress

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Physiological stress and injury are the primary reasons for increased outbreak of diseases in catfish. Since stress cannot be eliminated from culture practices entirely, researchers are investigating ways to decrease the stress response in order to increase survival to diseases. Booth and Peterson (...

  3. Effects of co-stocking smallmouth buffalo, Ictiobus bubalus, with channel catfish, Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Proliferative gill disease (PGD) in catfish is caused by the myxozoan Henneguya ictaluri. The complex life cycle requires Dero digitata as the oligochaete host. Efforts to control PGD by eradicating D. digitata have been unsuccessful. Smallmouth buffalo, Ictiobus bubalus, (SMB) are opportunistic bot...

  4. Gender nor sex hormones alter the disease susceptibility of channel catfish to Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The use of monosex populations for aquaculture is becoming widely used for several species. Monosex populations are not in wide use in the catfish industry but techniques to develop all male populations have been developed. These studies were conducted to determine if there were any differences be...

  5. Critical thermal maxima of two geographic strains of channel and hybrid catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Critical thermal maxima have been used extensively to provide physiologically and ecologically valuable reference points that identify early signs of thermal stress. In catfish pond culture, daily temperature maxima up to 36'C and daily fluctuations of as much as 6'Care observed. These extreme condi...

  6. Tolerance of Channel Catfish Fry to Abrupt pH Changes

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fry survival in the catfish industry is variable and failure to survive cannot be completely attributable to diseases, fertilization practices, or lack of zooplankton abundance. The variability in fry survival may be related to current handling and stocking methods. When stocked, fry are transferr...

  7. TLR5, NRAMP, TNF, AND Hepcidin Response to Challenge with Edwardsiella ictaluri in Channel Catfish Families with High and Low Susceptibility to Infection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Responses of toll-like receptor 5 (TLR5), tumor necrosis factor (TNF), natural resistance-associated macrophage protein (Nramp), and hepcidin to experimental challenge with Edwardsiella ictaluri in two families of channel catfish were measured in order to understand the mechanisms through which E. i...

  8. Expression profiles of toll-like receptors in anterior kidney of channel catfish Ictalurus punctatus (Rafinesque), acutely infected by Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Using quantitative PCR (QPCR), the relative transcriptional levels of five toll-like receptors (TLR2, TLR3, TLR5, TLR20a, and TLR21) were studied in the channel catfish, Ictalurus punctatus (Rafinesque), under normal and acutely infected conditions (1, 2, 4, 6, 12, 24, 36, and 48 h post injection (h...

  9. Effects of dietary supplementation of a purified nucleotide mixture on growth, immune function, and disease and stress resistance in channel catfish, Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Juvenile channel catfish (14.4 g average initial weight) were fed diets supplemented with a purified nucleotide mixture for 8 weeks. The mixture consisted of five nucleotides supplied on an equal basis as disodium salts at combined concentrations of 0 (control), 0.1, 0.3, 0.9, or 2.7% of diet. At th...

  10. Enhancement effects of dietary wheat distiller's dried grains with solubles on growth, immunity, and resistance to Edwardsiella ictaluri challenge of channel catfish, Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Juvenile channel catfish were fed for 12 weeks with diets containing wheat distiller’s dried grains with solubles (WDDGS) at levels of 0 (diet 1), 10, 20, 30 and 40% without (diets 2-5) or with (diets 6-9) lysine supplementation as substitutes of soybean and corn meal mixture. Fish fed diet 5 had th...

  11. Molecular identification and virulence of three Aeromonas hydrophila isolates cultured from infected channel catfish during a disease outbreak in West Alabama in 2009

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Three isolates (AL09-71, AL09-72, and AL09-73) of Aeromonas hydrophila were cultured from infected channel catfish during a disease outbreak in West Alabama in August 2009. Sequence analysis of 16S-23S rDNA intergenic spacer region (ISR), cpn60, gyrB, and rpoD genes of the three strains revealed tha...

  12. Development of phytoplankton communities and common off-flavors in a biofloc technology system used to culture channel catfish Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The use of biofloc technology production systems continues to increase in the aquaculture industry worldwide. Recent research demonstrated that outdoor biofloc systems can be used to produce high yields of channel catfish (Ictalurus punctatus). However, studies have not yet been performed to deter...

  13. Effects of fasting on IGF-I, IGF-II, and IGF-binding protein mRNA concentrations in channel catfish (Ictalurus punctatus)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The effects of fasting on IGF-I, IGF-II, and IGF-binding proteins (IGFBPs) in channel catfish were examined. Fed control fish (Fed) were compared to fish that had been fasted for 30 days followed by 15 days of additional feeding (Restricted). Sequence alignment and similarity to orthologous protei...

  14. Comparison of Estradiol, Testosterone, Vitellogenin and Cathepsin Profiles Among Two-year-old Channel Catfish (Ictalurus punctatus) Females from Four Selectively Bred Strains

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Potential variation in reproductive performance as young broodfish among four selectively bred strains of channel catfish was evaluated by comparing profiles of plasma estradiol, testosterone, and vitellogenin, and activities of cathepsin D, L, and B in follicle-enclosed oocytes during vitellogenesi...

  15. Molecular identfication and virulence of three Aeromonas hydrophila isolates cultured from infected channel catfish during a disease outbreak in West Alabama (USA) in 2009

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Three isolates (AL09-71, AL09-72, and AL09-73) of Aeromonas hydrophila were cultured from infected channel catfish during a disease outbreak in West Alabama in August 2009. Sequence analysis of 16S-23S rDNA intergenic spacer region (ISR), cpn60, gyrB, and rpoD genes of the three strains revealed tha...

  16. Channel catfish, Ictalurus punctatus, fed diets containing aflatoxin from moldy corn do not experience increased mortality after challenge with Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish, Ictalurus punctatus, fingerlings with an average body weight of 8.19±0.32 g were used to conduct this experiment to determine the effect of feeding diets containing aflatoxin on disease resistance. Twenty fish were randomly sorted into each of 48 80-L capacity flowing water aquaria....

  17. Channel Catfish (Ictalurus punctatus Rafinesque, 1818) CD156a (ADAM Metallopeptidase Domain 8): cDNA Clone, Characterization and Expression in Tissues

    Technology Transfer Automated Retrieval System (TEKTRAN)

    CD156a, also known as a disintegrin and metalloprotease domain 8 (ADAM-8), is a type 1 transmembrane glycoprotein of the ADAM family. This protein plays important roles in immune and other physiological functions. In this communication, the channel catfish CD156a cDNA was characterized and its exp...

  18. Genetic variation in feed consumption, growth, nutrient utilization efficiency and mitochondrial function within a farmed population of channel catfish Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We evaluated the effects of diets (32/4 or 36/6 percent protein/fat) and six channel catfish families for growth performance characteristics. Two families with fast- (C) and slow- (D) growth rate and with low and high feed efficiency (FE) were selected for analyses of mitochondrial complex enzymati...

  19. Sequence analysis, characterization and mRNA distribution of channel catfish (Ictalurus punctatus Rafinesque, 1818) chemokine (C-X-C Motif) receptor 4 (CXCR4) cDNA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Chemokine receptor CXCR4, a member of the G protein-coupled receptor superfamily, binds selectively CXCL12. This protein plays many important roles in immunological as well as pathophysiological functions. In this study, we identified and characterized the channel catfish CXCR4 transcript. The fu...

  20. Expression of leptin-like mRNA in channel catfish (Ictalurus punctatus) is induced by exposure to Edwardsiella ictaluri but is independent of energy status

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Leptin is a key pleiotropic cytokine involved in regulation of food intake and energy homeostasis in mammals. In channel catfish, the presence of a messenger RNA that encodes a leptin-like peptide (LLP) has been described. The objectives of our studies were to characterize tissue expression of LLP...

  1. Pond demonstration of production diets using high levels of distiller's dried grains with solubles with or without lysine supplementation for channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Due to the increased availability and potential price advantage of distiller's dried grains with solubles (DDGS), there is considerable interest in utilizing this product in aquaculture diets. The response of channel catfish Ictalurus punctatus to practical diets containing 20% and 30% DDGS with and...

  2. Growth performance, vitamin E status, and proximate and fatty acid composition of channel catfish, Ictalurus punctatus, fed diets containing various levels of fish oil and vitamin E

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A study was conducted to determine the effect of increasing dietary levels of fish oil on vitamin E requirement and their effect on growth performance, liver vitamin E status, and tissue proximate and fatty acid compositions of channel catfish. Basal purified diets (42% protein and 3,800 kcal DE/kg)...

  3. IMMUNIZATION OF EYED CHANNEL CATFISH, ICTALURUS PUNCTATUS, EGGS WITH MONOVALENT FLAVOBACTERIUM COLUMNARE VACCINE AND BIVALENT F. COLUMNARE AND EDWARDSIELLA ICTALURI VACCINE

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The efficacy of a modified live monovalent Flavobacterium columnare vaccine and bivalent F. columnare and Edwardsiella ictaluri vaccines were evaluated following immersion vaccination of eyed channel catfish (Ictalurus punctatus) eggs. The modified live F. columnare vaccine was grown in modified Sh...

  4. Production and associated economics of fingerling to stocker to growout modular phases for farming channel catfish, Ictalurus punctatus, in commercial size ponds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A 2-year study was conducted to evaluate fingerling to stocker (phase 2) and stocker to growout (phase 3) of three phases of a modular production system for channel catfish in commercial-scale ponds. Fingerlings (mean = 14.3 kg/1000, 11.9 cm) were stocked into each of six earthen ponds (1.62 ha) at ...

  5. Complete genome sequence of the highly virulent Aeromonas hydrophila AL09-71 isolated from disease channel catfish from West Alabama

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The highly virulent Aeromonas hydrophila isolate AL09-71 was cultured from infected channel catfish during the 2009 disease outbreak in West Alabama. The complete genome of this highly virulent Aeromonas hydrophila isolate AL09-71 is 5,023,861 bp in size. The genome has 4489 coding sequences, 11 cop...

  6. Development of phytoplankton communities and common off-flavors in a biofloc technology system used for the culture of channel catfish (Ictalurus punctatus)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The use of biofloc technology production systems continues to increase in the aquaculture industry worldwide. Recent research demonstrated that outdoor biofloc systems can be used to produce high yields of channel catfish (Ictalurus punctatus). However, studies have not yet been performed to deter...

  7. Genome sequence of Edwardsiella ictaluri 93-146 a strain associated with a natural channel catfish outbreak of enteric septicemia of catifsh

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Edwardsiella ictaluri is the cause of extensive mortalities and economic losses to the channel catfish industry of the southeast United States. Here we report the complete genome of Edwardsiella ictaluri 93-146. Whole-genome sequence analysis of E. ictaluri provides a tool for understanding the geno...

  8. Identification and expression profile of multiple genes in the anterior kidney of channel catfish induced by modified live Edwardsiella ictaluri vaccination

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Using PCR-select subtractive cDNA hybridization technique, 57 expressed sequence tags (ESTs) were isolated from 240 clones of a modified live Edwardsiella ictaluri-vaccinated vs sham-vaccinated channel catfish anterior kidney subtractive library. The transcription levels of the 57 ESTs in response t...

  9. Influence of dietary levels of magnesium on growth, tissue mineral content, and resistance of channel catfish Ictalurus punctatus challenged with Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Juvenile channel catfish were fed purified diets supplemented with magnesium (Mg) from Mg sulfate at levels of 0, 200, 400, 600, 800 and 1,000 mg/kg and 0, 200, 400, 600 and 800 mg/kg in two separate feeding studies. In study I, the effect of dietary levels of Mg on growth response, vertebral miner...

  10. Identification of Two Cholecystokinin mRNA in Channel Catfish: Their Tissue Distribution and Changes in Their Expression After Prolonged Fasting and Refeeding

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Food intake in fish is regulated by complex interactions of neural and endocrine factors. Our previous studies showed that channel catfish selected for increased growth consumed more food than those with lower growth. However, mechanisms that resulted in increased food intake of fish selected for ...

  11. Effects of Dried Algae Schizochytrium Sp., A Rich Source of Docosahexaenoic Acid, on Growth, Fatty Acid Composition, and Sensory Quality of Channel Catfish Ictalurus Punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A laboratory study was conducted to investigate the effect of supplementation of dried algae Schizochytrium sp., a rich source of 22:6 n-3, on growth, fatty acid composition, and sensory quality of channel catfish Ictalurus punctatus. Five isonitrogenous (28% crude protein) and isocaloric (2.78 kcal...

  12. Effects of dietary fiber concentrations supplied by corn bran on feed intake growth and feed efficiency of channel catfish Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The present study examined the effects of dietary fiber and digestible energy on the feed intake, growth, and feed efficiency of juvenile channel catfish Ictalurus punctatus. Fish with an initial weight of 9.8 ± 0.1 g/fish (mean ± SD) were stocked in 110-L flow-through aquariums and fed for 9 weeks ...

  13. Effect of stocking large channel catfish in a biofloc technology production system on production and incidence of common microbial off-flavor compounds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Density-dependent production and incidence of common microbial off-flavors caused by geosmin and 2-methylisoborneol were investigated in an outdoor biofloc technology production system stocked with stocker-size (217 g/fish) channel catfish at 1.4, 2.1, or 2.8 kg/m3. Individual weight at harvest rang...

  14. cDNA sequences of channel catfish (Ictalurus punctatus Rafinesque, 1818) annexin A2, A4, A5 and A11

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Annexins, a protein superfamily, are ubiquitous, and play many important roles in immuno-physiological processes. In this report, we cloned and sequenced channel catfish orthologs to human annexin A2, A4, A5 and A11. Total RNA from tissues was extracted and cDNA libraries were constructed by the r...

  15. Growth and Feed Efficiency of Juvenile Channel Catfish Reared at Different Water Temperatures and Fed Diets Containing Various Levels of Fish Meal

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish Ictalurus punctatus do not feed well at low temperatures. It is thought that a diet containing fish meal may enhance feed palatability at low temperatures since fish meal is highly palatable to the fish. There is a lack of information on effects of fish meal levels on growth perfor...

  16. The cytochrome P450 genes of channel catfish: their involvement in disease defense responses as revealed by meta-analysis of RNA-Seq datasets

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cytochrome P450s (CYPs) encode one of the most diverse enzyme superfamily in nature. They catalyze oxidative reactions of endogenous molecules and exogenous chemicals. Methods: We identifiedCYPs genes through in silico analysis using EST, RNA-Seq and genome databases of channel catfish.Phylogenetic ...

  17. Growth Performance and Resistance to Edwardsiella ictaluri of Channel Catfish (Ictalurus punctatus)Fed Diets Containing Distiller's Dried Grains with Solubles

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A study was conducted to examine the effect of dietary levels of distiller’s dried grains with solubles (DDGS) on growth, body composition, hematology, immune response and resistance of channel catfish, Ictalurus punctatus, to Edwardsiella ictaluri challenge. Five diets containing 0, 10, 20, 30 and ...

  18. Comparative transcriptional analysis reveals distinct expression patterns of channel catfish genes after the first infection and re-infection with Aeromonas hydrophila

    Technology Transfer Automated Retrieval System (TEKTRAN)

    To determine whether transcriptional levels of channel catfish (Ictalurus punctatus) genes are differentially regulated between a first infection with Aeromonas hydrophila and a re-infection, suppression subtractive hybridization (SSH) was performed in this study using anterior kidney cDNA after the...

  19. Effects of dietary supplementation of a purified nucleotide mixture on immune function and disease and stress resistance in channel catfish, Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Juvenile channel catfish (14.4 g average initial weight) were fed diets supplemented with a purified nucleotide mixture for 8 weeks. The mixture consisted of five nucleotides supplied on an equal basis as disodium salts at combined concentrations of 0 (control), 0.1, 0.3, 0.9, or 2.7% of diet. At th...

  20. Evaluation of the therapeutic effect of potassium permanganate at early stages of an experimental acute infection of Flavobacterium columnare in channel catfish (Ictalurus punctatus)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The efficacy of potassium permanganate (KMnO4) against early stages of an experimental acute infection of Flavobacterium columnare in channel catfish (Ictalurus punctatus) was evaluated. Fish were experimentally challenged, by waterborne exposure for 2 h to F. columnare after cutaneous abrasion, an...

  1. Identification and expression profile of multiple genes in the anterior kidney of channel catfish induced by modified live Edwardsiella ictaluri vaccination.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Using PCR-select subtractive cDNA hybridization technique, 57 expressed sequence tags (ESTs) were isolated from 240 clones of a modified live Edwardsiella ictaluri-vaccinated vs sham-vaccinated channel catfish anterior kidney subtractive library. The transcription levels of the 57 ESTs in response t...

  2. Determination of niclosamide residues in rainbow trout (Oncorhynchus mykiss) and channel catfish (Ictalurus punctatus) fillet tissue by high-performance liquid chromatography

    USGS Publications Warehouse

    Schreier, T.M.; Dawson, V.K.; Cho, Yirang; Spanjers, N.J.; Boogaard, M.A.

    2000-01-01

    Bayluscide [the ethanolamine salt of niclosamide (NIC)] is a registered piscicide used in combination with 3-(trifluoromethyl)-4-nitrophenol (TFM) to control sea lamprey populations in streams tributary to the Great Lakes. A high-performance liquid chromatography (HPLC) method was developed for the determination of NIC residues in muscle fillet tissues of fish exposed to NIC and TFM during sea lamprey control treatments. NIC was extracted from fortified channel catfish and rainbow trout fillet tissue with a series of acetone extractions and cleaned up on C-18 solid-phase extraction cartridges. NIC concentrations were determined by HPLC with detection at 360 and 335 nm for rainbow trout and catfish, respectively. Recovery of NIC from rainbow trout (n = 7) fortified at 0.04 mu g/g was 77 +/- 6.5% and from channel catfish (n = 7) fortified at 0.02 mu g/g was 113 +/- 11%. NIC detection limit was 0.0107 mu g/g for rainbow trout and 0.0063 mu g/g for catfish. Percent recovery of incurred radioactive residues by this method from catfish exposed to [C-14]NIC was 89.3 +/- 4.1%. Percent recoveries of NIC from fortified storage stability tissue samples for rainbow trout (n = 3) analyzed at 5 and 7.5 month periods were 78 +/- 5.1 and 68 +/- 2.4%, respectively. Percent recoveries of NIC from fortified storage stability tissue samples for channel catfish (n = 3) analyzed at 5 and 7.5 month periods were 88 +/- 13 and 76 +/- 21%, respectively.

  3. Variations in abundance of young-of-the-year channel catfish in a navigation pool of the upper Mississippi River

    USGS Publications Warehouse

    Holland Bartels, L. E.; Duval, M.C.

    1988-01-01

    Spatial and temporal variations in the abundance of young-of-the-year channel catfish Ictalurus punctatus were determined over 3 years based on 154 trawls taken from Navigation Pool 7 of the upper Mississippi River. No significant spatial patterns in abundance or length of fish in the catch were found in seven poolwide surveys of the main channel. However, high variation among replicates existed. Temporal patterns in abundance and length-frequency distributions varied among the study years. Seasonal catch curves were similar in 1984 and 1986, although offset due to earlier spawning in 1986. Spawning appeared to have been protracted in both years, based on analyses of back-calculated dates of spawn and length-frequency patterns of young. Sixty to seventy percent more young were produced in 1985, when spawning occurred over a shorter period of time and a more uniformly sized year class was produced.

  4. Distribution and elimination of [14C] sarafloxacin hydrochloride from tissues of juvenile channel catfish (Ictalurus punctatus)

    USGS Publications Warehouse

    Gingerich, W.H.; Meinertz, J.R.; Dawson, V.K.; Gofus, J.E.; Delaney, L.J.; Bunnell, P.R.

    1995-01-01

    The distribution and loss of radioactivity from tissues were determined in 60 juvenile channel catfish (Ictalurus punctatus) following oral dosing with the candidate fish therapeutant Sarafin® ([14C] sarafloxacin hydrochloride) at 10 mg/kg for 5 consecutive days. Twelve groups of 5 fish each were sampled at selected times ranging from 3 to 240 h after the last dose was administered, The concentration and content of sarafloxacin-equivalent activity was determined in liver, gallbladder, kidney, skin, and skinless fillet by sample oxidation and liquid scintillation counting; content of sarafloxacin-equivalent activity was determined in stomach and anterior and posterior intestines, Skinless fillet tissues were also analyzed for sarafloxacin and for potential metabolites by gradient-elution high-performance liquid chromatography (HPLC) with in-line radiometric and fluorescence detection, Loss of radioactivity from the whole body conformed to a bimodal elimination pattern with a rapid initial phase (t1/2=11 h) and a slower secondary phase (t1/2=222 h). Tissue and contents of the gastrointestinal tract (i.e. stomach and anterior and posterior intestines) were a principal depot of activity during the first four sample times (3, 6, 12, and 24 h); the combined head, skeleton, and fins (i.e. residual carcass) were the principal depot of activity in samples taken after 24 h. Of those tissues sampled 3 h after the last dose, relative sarafloxacin concentration was greatest in the liver (4.06 μg equivalents/g) and least in the residual carcass (1.13 μg equivalents/g), Intermediate concentrations were found in the kidney (2.04 μg equivalents/g), skinless fillet (1.71 μg equivalents/ g), and the skin (1.51 μg equivalents/g). Concentrations of sarafloxacin-equivalent residues in edible skinless fillet were consistently among the lowest of all tissues examined. The highest mean concentration of parent-equivalent material in the fillet tissue was found 12 h after

  5. POTENTIAL FOR GREAT EGRETS (ARDEA ALBA) TO TRANSMIT A VIRULENT STRAIN OF AEROMONAS HYDROPHILA AMONG CHANNEL CATFISH (ICTALURUS PUNCTATUS) CULTURE PONDS.

    PubMed

    Jubirt, Madison M; Hanson, Larry A; Hanson-Dorr, Katie C; Ford, Lorelei; Lemmons, Scott; Fioranelli, Paul; Cunningham, Fred L

    2015-07-01

    Aeromonas hydrophila is a gram-negative, rod-shaped, facultative, anaerobic bacterium that is ubiquitous in freshwater and slightly brackish aquatic environments and infects fish, humans, reptiles, and birds. Recent severe outbreaks of disease in commercial channel catfish (Ictalurus punctatus) aquaculture ponds have been associated with a highly virulent A. hydrophila strain (VAH), which is genetically distinct from less-virulent strains. The epidemiology of this disease has not been determined. Given that A. hydrophila infects birds, we hypothesized that fish-eating birds may serve as a reservoir for VAH and spread the pathogen by flying to uninfected ponds. Great Egrets (Ardea alba) were used in this transmission model because these wading birds frequently prey on farmed catfish. Great Egrets that were fed VAH-infected catfish shed VAH in feces demonstrating their potential to spread VAH. PMID:25984772

  6. In vivo responses of single olfactory receptor neurons of channel catfish to binary mixtures of amino acids.

    PubMed

    Kang, J; Caprio, J

    1997-01-01

    For the first time in any vertebrate, in vivo responses of single olfactory receptor neurons to odorant mixtures were studied quantitatively. Extracellular electrophysiological response of 54 single olfactory receptor neurons from 23 channel catfish, Ictalurus punctatus, to binary mixtures of amino acids and to their components were recorded simultaneously with the electroolfactogram (EOG). For 57% (73 of 128) of the tests, no significant change (N) from spontaneous activity occurred. Responses to the remaining 55 tests of binary mixtures were excitatory (E; 13%) or suppressive (S; 30%). No response type was associated with any specific mixture across the neurons sampled. Eighty-six percent of the responses of catfish olfactory receptor neurons to binary mixtures were classified similar to at least one of the component responses, a percentage comparable (i.e., 89%) with that observed for single olfactory bulb neurons in the same species to equivalent binary mixtures. The responses of single olfactory receptor neurons to component-similar binary mixtures (i.e., component responses were both E, both S, and both N, respectively) were generally (80% of 59 tests) classified similar to the responses to the components. For E+N and S+N binary mixtures, the N component often (66% of 58 tests) reduced or concealed (i.e., "masked") the excitatory and suppressive responses, respectively. For the majority (6 of 11 tests) of E + S binary mixtures, null activity resulted. Responses to the remaining five tests were either excitatory (n = 3) or suppressive (n = 2). PMID:9120550

  7. Influence of Dietary Coexposure to Benzo(a)pyrene on the Biotransformation and Distribution of 14C-Methoxychlor in the Channel Catfish (Ictalurus punctatus)

    PubMed Central

    Nyagode, Beatrice A.; James, Margaret O.; Kleinow, Kevin M.

    2009-01-01

    Methoxychlor (MXC) is an organochlorine pesticide whose mono- and bis-demethylated metabolites, 2-(4-hydroxyphenyl)-2-(4-methoxyphenyl)-1,1,1-trichloroethane (OH-MXC) and 2,2-bis(4-hydroxyphenyl)-1,1,1-trichloroethane (HPTE), respectively, are estrogenic and antiandrogenic. Studies in vitro showed that treatment of channel catfish with a polycyclic aromatic hydrocarbon increased phase I and phase II metabolism of MXC. To determine the in vivo significance, groups of four channel catfish were treated by gavage for 6 days with 2 mg/kg 14C-MXC alone or 2 mg/kg 14C-MXC and 2 mg/kg benzo(a)pyrene (BaP). On day 7, blood and tissue samples were taken for analysis. Hepatic ethoxyresorufin O-deethylase activity was 10-fold higher in the BaP-treated catfish, indicating CYP1A induction. More MXC-derived radioactivity remained in control (42.8 ± 4.1%) than BaP-induced catfish (28.5 ± 3.2%), mean percent total dose ± SE. Bile, muscle and fat contained approximately 90% of the radioactivity remaining in control and induced catfish. Extraction and chromatographic analysis showed that liver contained MXC, OH-MXC, HPTE, and glucuronide but not sulfate conjugates of OH-MXC and HPTE. Liver mitochondria contained more MXC, OH-MXC, and HPTE than other subcellular fractions. Bile contained glucuronides of OH-MXC and HPTE, and hydrolysis of bile gave HPTE and both enantiomers of OH-MXC. The muscle, visceral fat, brain and gonads contained MXC, OH-MXC, and HPTE in varying proportions, but no conjugates. This study showed that catfish coexposed to BaP and MXC retained less MXC and metabolites in tissues than those exposed to MXC alone, suggesting that induction enhanced the elimination of MXC, and further showed that potentially toxic metabolites of MXC were present in the edible tissues. PMID:19181613

  8. Antioxidative activity of protein hydrolysates prepared from alkaline-aided channel catfish protein isolates.

    PubMed

    Theodore, Ann E; Raghavan, Sivakumar; Kristinsson, Hordur G

    2008-08-27

    Antioxidative activity of hydrolyzed protein prepared from alkali-solubilized catfish protein isolates was studied. The isolates were hydrolyzed to 5, 15, and 30% degree of hydrolysis using the protease enzyme, Protamex. Hydrolyzed protein was separated into hydrolysates and soluble supernatants, and both of these fractions were studied for their metal chelating ability, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability, ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), and their ability to inhibit the formation of thiobarbituric acid reactive substances (TBARS) in washed tilapia muscle containing tilapia hemolysate. Both hydrolysates and supernatants were characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Results showed that DPPH radical scavenging ability and reducing power of catfish protein hydrolysates decreased, whereas the ORAC value, metal chelating ability, and ability to inhibit TBARS increased, with an increase in the degree of hydrolysis. Hydrolysate samples showed higher DPPH radical scavenging ability and Fe(3+) reducing ability, and supernatant samples had higher metal chelating ability. In general, low molecular weight (MW) peptides had high ORAC values and high metal chelating ability, and high MW peptides had a higher reducing power (FRAP) and were more effective in scavenging DPPH radicals. In a washed muscle model system, the ability of catfish protein hydrolysates and their corresponding supernatants to inhibit the formation of TBARS increased with an increase in the degree of hydrolysis. PMID:18662014

  9. Producing Monosex Male Populations for Catfish Culture

    Technology Transfer Automated Retrieval System (TEKTRAN)

    There are a number of uses for the preferential production of male catfish in aquaculture operations. Several studies have demonstrated that male channel catfish grow faster than female fish and would be expected to reach market size faster. Also, in the production of hybrid catfish male blue catf...

  10. Vaccination against enteric septicaemia of catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Edwardsiella ictaluri, the causative agent of enteric septicemia (ESC) of catfish, is one of the most economically important diseases of cultured channel catfish. In 2002, Wagner and coworkers reported that enteric septicemia of catfish (ESC) and columnaris (Flavobacterium columnaris) were the two m...

  11. Water quality in hybrid catfish ponds after partial fish harvest

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Intensification of United States catfish aquaculture involves hybrid catfish ('channel catfish Ictalurus punctatus x ' blue catfish I. furcatus) grown in ponds with abundant aeration and high feeding rates. High feeding rates cause water quality deterioration because most of the nitrogen, phosphorus...

  12. Adjuvant Immune Enhancement of Subunit Vaccine Encoding pSCPI of Streptococcus iniae in Channel Catfish (Ictalurus punctatus).

    PubMed

    Jiang, Jie; Zheng, Zonglin; Wang, Kaiyu; Wang, Jun; He, Yang; Wang, Erlong; Chen, Defang; Ouyang, Ping; Geng, Yi; Huang, Xiaoli

    2015-01-01

    Channel catfish (Ictalurus punctatus) is an important agricultural fish that has been plagued by Streptococcus iniae (S. iniae) infections in recent years, some of them severe. C5a peptidase is an important virulent factor of S. iniae. In this study, the subunit vaccine containing the truncated part of C5a peptidase (pSCPI) was mixed with aluminum hydroxide gel (AH), propolis adjuvant (PA), and Freund's Incomplete Adjuvant (FIA). The immunogenicity of the pSCPI was detected by Western-blot in vitro. The relative percent survival (RPS), lysozyme activity, antibody titers, and the expression of the related immune genes were monitored in vivo to evaluate the immune effects of the three different adjuvants. The results showed that pSCPI exerted moderate immune protection (RPS = 46.43%), whereas each of the three adjuvants improved the immune protection of pSCPI. The immunoprotection of pSCPI + AH, pSCPI + PA, and pSCPI + FIA was characterized by RPS values of 67.86%, 75.00% and, 85.71%, respectively. Further, each of the three different adjuvanted pSCPIs stimulated higher levels of lysozyme activity and antibody titers than the unadjuvanted pSCPI and/or PBS buffer. In addition, pSCPI + FIA and pSCPI + PA induced expression of the related immune genes under investigation, which was substantially higher than the levels stimulated by PBS. pSCPI + AH significantly stimulated the induction of MHC II β, CD4-L2, and IFN-γ, while it induced slightly higher production of TNF-α and even led to a decrease in the levels of IL-1β, MHC I α, and CD8 α. Therefore, we conclude that compared with the other two adjuvants, FIA combined with pSCPI is a more promising candidate adjuvant against S. iniae in channel catfish. PMID:26602918

  13. Adjuvant Immune Enhancement of Subunit Vaccine Encoding pSCPI of Streptococcus iniae in Channel Catfish (Ictalurus punctatus)

    PubMed Central

    Jiang, Jie; Zheng, Zonglin; Wang, Kaiyu; Wang, Jun; He, Yang; Wang, Erlong; Chen, Defang; Ouyang, Ping; Geng, Yi; Huang, Xiaoli

    2015-01-01

    Channel catfish (Ictalurus punctatus) is an important agricultural fish that has been plagued by Streptococcus iniae (S. iniae) infections in recent years, some of them severe. C5a peptidase is an important virulent factor of S. iniae. In this study, the subunit vaccine containing the truncated part of C5a peptidase (pSCPI) was mixed with aluminum hydroxide gel (AH), propolis adjuvant (PA), and Freund’s Incomplete Adjuvant (FIA). The immunogenicity of the pSCPI was detected by Western-blot in vitro. The relative percent survival (RPS), lysozyme activity, antibody titers, and the expression of the related immune genes were monitored in vivo to evaluate the immune effects of the three different adjuvants. The results showed that pSCPI exerted moderate immune protection (RPS = 46.43%), whereas each of the three adjuvants improved the immune protection of pSCPI. The immunoprotection of pSCPI + AH, pSCPI + PA, and pSCPI + FIA was characterized by RPS values of 67.86%, 75.00% and, 85.71%, respectively. Further, each of the three different adjuvanted pSCPIs stimulated higher levels of lysozyme activity and antibody titers than the unadjuvanted pSCPI and/or PBS buffer. In addition, pSCPI + FIA and pSCPI + PA induced expression of the related immune genes under investigation, which was substantially higher than the levels stimulated by PBS. pSCPI + AH significantly stimulated the induction of MHC II β, CD4-L2, and IFN-γ, while it induced slightly higher production of TNF-α and even led to a decrease in the levels of IL-1β, MHC I α, and CD8 α. Therefore, we conclude that compared with the other two adjuvants, FIA combined with pSCPI is a more promising candidate adjuvant against S. iniae in channel catfish. PMID:26602918

  14. Impact of feed additives on surface mucosal health and columnaris susceptibility in channel catfish fingerlings, Ictalurus punctatus.

    PubMed

    Zhao, Honggang; Li, Chao; Beck, Benjamin H; Zhang, Ran; Thongda, Wilawan; Davis, D Allen; Peatman, Eric

    2015-10-01

    One of the highest priority areas for improvement in aquaculture is the development of dietary additives and formulations which provide for complete mucosal health and protection of fish raised in intensive systems. Far greater attention has been paid to dietary impact on gut health than to protective effects at other mucosal surfaces such as skin and gill. These exterior surfaces, however, are important primary targets for pathogen attachment and invasion. Flavobacterium columnare, the causative agent of columnaris disease, is among the most prevalent of all freshwater disease-causing bacteria, impacting global aquaculture of catfish, salmonids, baitfish and aquaria-trade species among others. This study evaluated whether the feeding of a standard catfish diet supplemented with Alltech dietary additives Actigen(®), a concentrated source of yeast cell wall-derived material and/or Allzyme(®) SSF, a fermented strain of Aspergillus niger, could offer protection against F. columnare mortality. A nine-week feeding trial of channel catfish fingerlings with basal diet (B), B + Allzyme(®) SSF, B + Actigen(®) and B + Actigen(®)+Allzyme(®) SSF revealed good growth in all conditions (FCR < 1.0), but no statistical differences in growth between the treatments were found. At nine weeks, based on pre-challenge trial results, basal, B + Actigen(®), and B + Allzyme(®) SSF groups of fish were selected for further challenges with F. columnare. Replicated challenge with a virulent F. columnare strain, revealed significantly longer median days to death in B + Allzyme(®) SSF and B + Actigen(®) when compared with the basal diet (P < 0.05) and significantly higher survival following the eight day challenge period in B + Actigen(®) when compared with the other two diets (P < 0.05). Given the superior protection provided by the B + Actigen(®) diet, we carried out transcriptomic comparison of gene expression of fish fed that diet and the basal diet before and after columnaris

  15. Construction of a high-density, high-resolution genetic map and its integration with BAC-based physical map in channel catfish

    PubMed Central

    Li, Yun; Liu, Shikai; Qin, Zhenkui; Waldbieser, Geoff; Wang, Ruijia; Sun, Luyang; Bao, Lisui; Danzmann, Roy G.; Dunham, Rex; Liu, Zhanjiang

    2015-01-01

    Construction of genetic linkage map is essential for genetic and genomic studies. Recent advances in sequencing and genotyping technologies made it possible to generate high-density and high-resolution genetic linkage maps, especially for the organisms lacking extensive genomic resources. In the present work, we constructed a high-density and high-resolution genetic map for channel catfish with three large resource families genotyped using the catfish 250K single-nucleotide polymorphism (SNP) array. A total of 54,342 SNPs were placed on the linkage map, which to our knowledge had the highest marker density among aquaculture species. The estimated genetic size was 3,505.4 cM with a resolution of 0.22 cM for sex-averaged genetic map. The sex-specific linkage maps spanned a total of 4,495.1 cM in females and 2,593.7 cM in males, presenting a ratio of 1.7 : 1 between female and male in recombination fraction. After integration with the previously established physical map, over 87% of physical map contigs were anchored to the linkage groups that covered a physical length of 867 Mb, accounting for ∼90% of the catfish genome. The integrated map provides a valuable tool for validating and improving the catfish whole-genome assembly and facilitates fine-scale QTL mapping and positional cloning of genes responsible for economically important traits. PMID:25428894

  16. Construction of a high-density, high-resolution genetic map and its integration with BAC-based physical map in channel catfish.

    PubMed

    Li, Yun; Liu, Shikai; Qin, Zhenkui; Waldbieser, Geoff; Wang, Ruijia; Sun, Luyang; Bao, Lisui; Danzmann, Roy G; Dunham, Rex; Liu, Zhanjiang

    2015-02-01

    Construction of genetic linkage map is essential for genetic and genomic studies. Recent advances in sequencing and genotyping technologies made it possible to generate high-density and high-resolution genetic linkage maps, especially for the organisms lacking extensive genomic resources. In the present work, we constructed a high-density and high-resolution genetic map for channel catfish with three large resource families genotyped using the catfish 250K single-nucleotide polymorphism (SNP) array. A total of 54,342 SNPs were placed on the linkage map, which to our knowledge had the highest marker density among aquaculture species. The estimated genetic size was 3,505.4 cM with a resolution of 0.22 cM for sex-averaged genetic map. The sex-specific linkage maps spanned a total of 4,495.1 cM in females and 2,593.7 cM in males, presenting a ratio of 1.7 : 1 between female and male in recombination fraction. After integration with the previously established physical map, over 87% of physical map contigs were anchored to the linkage groups that covered a physical length of 867 Mb, accounting for ∼90% of the catfish genome. The integrated map provides a valuable tool for validating and improving the catfish whole-genome assembly and facilitates fine-scale QTL mapping and positional cloning of genes responsible for economically important traits. PMID:25428894

  17. Distribution, Abundance, and Population Dynamics of Northern Squawfish, Walleye, Smallmouth Bass, and Channel Catfish in John Day Reservoir, 1986 Annual Report.

    SciTech Connect

    Beamesderfer, Raymond C.

    1987-04-01

    John Day Reservoir was sampled from 25 March to 1 September 1986 using gill nets, trap nets, boat electrofishers, hook and line, and an angler survey to collect 4945 northern squawfish Ptychocheilus oregonesis, 602 walleye Stizostedion vitreum 2894 smallmouth bass Micropterus dolomieui, and 563 channel catfish Icatalurus punctatus. Distribution, abundance and population parameters of each species were examined. One year growth, mortality, and relative year class strength was described.

  18. Roles for mannose binding lectin and rhamnose binding lectin in channel catfish fed essential oils and challenged with Edwardsiella ictaluri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A major problem in the catfish farming industry has been high disease loss to enteric septicemia of catfish (ESC), caused by the bacterium Edwardsiella ictaluri. Methods to control this disease include vaccination, antibiotic therapy, and restricted feeding. Another method that has been examined i...

  19. Implication of lateral genetic transfer in the emergence of Aeromonas hydrophila isolates of epidemic outbreaks in channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Background: A recent epidemic outbreak of motile Aeromonas septicemia of catfish caused by highly virulent Aeromonas hydrophila is a major threat to the catfish industry in the southeastern United States. The lack of a complete genome sequence for this newly emerged A. hydrophila genotype hampers ef...

  20. Highly specific olfactory receptor neurons for types of amino acids in the channel catfish.

    PubMed

    Nikonov, Alexander A; Caprio, John

    2007-10-01

    Odorant specificity to l-alpha-amino acids was determined electrophysiologically for 93 single catfish olfactory receptor neurons (ORNs) selected for their narrow excitatory molecular response range (EMRR) to only one type of amino acid (i.e., Group I units). These units were excited by either a basic amino acid, a neutral amino acid with a long side chain, or a neutral amino acid with a short side chain when tested at 10(-7) to 10(-5) M. Stimulus-induced inhibition, likely for contrast enhancement, was primarily observed in response to the types of amino acid stimuli different from that which activated a specific ORN. The high specificity of single Group I ORNs to type of amino acid was also previously observed for single Group I neurons in both the olfactory bulb and forebrain of the same species. These results indicate that for Group I neurons olfactory information concerning specific types of amino acids is processed from receptor neurons through mitral cells of the olfactory bulb to higher forebrain neurons without significant alteration in unit odorant specificity. PMID:17686913

  1. HPLC determination and MS confirmation of malachite green, gentian violet, and their leuco metabolite residues in channel catfish muscle.

    PubMed

    Chen, Guoying; Miao, Shui

    2010-06-23

    Residues of malachite green (MG), gentian violet (GV), and their leuco metabolites in channel catfish muscle were individually determined by HPLC using diode array and fluorescence detectors and confirmed by tandem mass spectrometry. This detection scheme obviates a PbO(2) reactor that converts leuco forms to chromatic forms for absorbance detection, therefore eliminating uncertainties in oxidant depletion and data integrity. Extraction was performed once in pH 3 McIlvaine buffer and acetonitrile, followed by cleanup using a polymeric strong cation-exchange column. Liquid-liquid extraction was excluded to provide an environmentally responsible and relatively rapid protocol. Spectrometric limits of detection (LOD; S/N = 3) for MG (lambda = 620 nm) and GV (lambda = 588 nm) were 0.38 and 0.26 ng/g with 44.5-49.2% and 92.2-101.4% recoveries (1-10 ng/g, n = 6), respectively. Fluorometric LOD (S/N = 3) for LMG and LGV (lambda(ex) = 266 nm, lambda(em) = 360 nm) were 0.10 and 0.09 ng/g with 74.3-84.5% and 80.6-86.5% recoveries (1-10 ng/g, n = 6), respectively. This simplified protocol saves costs and meets the sensitivity requirements set by the Food and Drug Administration and the European Union. PMID:20481609

  2. Development of a Selection Index on Blue Catfish for Optimized Sperm Production in the Catfish X Blue Hybrid Catfish Production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel x blue hybrid catfish is a phenomenal fish for pond culture. This fish represents a major improvement in catfish production efficiency due to higher growth rates, lower feed conversion efficiency and improved disease resistance and processing yield compared to the commonly raised channel ca...

  3. Channel catfish granzyme-like I is a highly specific serine protease with metase activity that is expressed by fish NK-like cells.

    PubMed

    Thorpe, Michael; Akula, Srinivas; Hellman, Lars

    2016-10-01

    Here we present the extended cleavage specificity of catfish granzyme-like I, previously identified in fish NK-like cells. This protease has been characterised using substrate phage display and further validated by using a panel of recombinant substrates. A strict preference for Met in the P1 (cleavage) position, indicating metase specificity was observed. A screening of potential in vivo substrates was performed based on the derived P5-P3' consensus: Arg-Val-Thr-Gly-Met(↓)Ser-Leu-Val. Channel catfish caspase 6 was one very interesting potential target identified. This site was present in an adjacent position to the classic caspase activation site (Asp179 in human caspase 6). Cleavage of this site (hence potential activation) by the catfish granzyme-like I could reveal a novel mechanism of caspase 6 activation. This poses an interesting idea that the role of granzyme-like proteases in the activation of caspase dependent apoptosis mechanisms has been conserved for over 400 million years. PMID:27216028

  4. A dietary dairy/yeast prebiotic and flaxseed oil enhance growth, hematological and immunological parameters in channel catfish at a suboptimal temperature (15°C).

    PubMed

    Thompson, M; Lochmann, R; Phillips, H; Sink, T D

    2015-07-01

    Channel catfish raised in the southern United States require two growing seasons to reach market size. Growing seasons are separated by a cool period of about 3 months when feed intake and growth are greatly reduced. A cool-weather feeding strategy to improve feed intake, growth or health of catfish might improve survival and reduce the time needed to achieve market size. We conducted a feeding trial with channel catfish at a suboptimal temperature (15°C) to determine the effects of supplementing diets with either a dairy/yeast prebiotic or flaxseed oil (high in 18:3n-3) compared with a control with soybean oil (high in 18:2n-6). The trial was conducted in recirculating systems with 1140-l tanks containing 100 fish each (mean initial weight 61.4 g±0.43 s.e.m.). A 28%-protein basal diet was supplemented with 20 g/kg cellulose and 20 g/kg soybean oil (SBO, control), 20 g/kg cellulose and 20 g/kg flaxseed oil (FLAX) or 20 g/kg of a dairy/yeast prebiotic and 20 g/kg soybean oil (PREB). Fish were fed once daily to satiation and weighed every 3 weeks to track growth. Hematology, non-specific immune responses, proximate and fatty acid composition of muscle were determined to assess diet effects. Catfish-fed FLAX or PREB had higher weight gain, feed consumption and lysozyme activity than fish fed SBO. Total n-3 fatty acids in muscle were higher in fish fed SBO or FLAX than those fed PREB. Total n-6 long-chain polyunsaturated acids were higher in muscle of fish fed PREB than those fed SBO. Fatty acids in the PREB and SBO diets were similar, so the PREB appeared to increase elongation and desaturation of n-6 fatty acids in muscle. Flaxseed oil and the dairy/yeast prebiotic both have potential to increase catfish performance at a low temperature. PMID:25743337

  5. Electro-olfactogram and multiunit olfactory receptor responses to complex mixtures of amino acids in the channel catfish, Ictalurus punctatus.

    PubMed

    Kang, J S; Caprio, J

    1991-10-01

    In vivo electrophysiological recordings from populations of olfactory receptor neurons in the channel catfish, Ictalurus punctatus, clearly showed that both electro-olfactogram and integrated neural responses of olfactory receptor cells to complex mixtures consisting of up to 10 different amino acids were predictable with knowledge of (a) the responses to the individual components in the mixture and (b) the relative independence of the respective receptor sites for the component stimuli. All amino acid stimuli used to form the various mixtures were initially adjusted in concentration to provide approximately equal response magnitudes. Olfactory receptor responses to both multimixtures and binary mixtures were recorded. Multimixtures were formed by mixing equal aliquots of 3-10 different amino acids. Binary mixtures were formed by mixing equal aliquots of two equally stimulatory solutions. Solution 1 contained either one to nine different neutral amino acids with long side-chains (LCNs) or one to five different neutral amino acids with short side-chains (SCNs). Solution 2, comprising the binary mixture, consisted of only a single stimulus, either a LCN, SCN, basic, or acidic amino acid. The increasing magnitude of the olfactory receptor responses to mixtures consisting of an increasing number of neutral amino acids indicated that multiple receptor site types with highly overlapping specificities exist to these compounds. For both binary mixtures and multimixtures composed of neutral and basic or neutral and acidic amino acids, the receptor responses were significantly enhanced compared with those mixtures consisting of an equal number of only neutral amino acids. These results demonstrate that receptor sites for the basic and acidic amino acids, respectively, are highly independent of those for the neutral amino acids, and suggest that a mechanism for synergism is the simultaneous activation of relatively independent receptor sites by the components in the mixture

  6. The effect of copper sulfate, potassium permanganate, and peracetic acid on Ichthyobodo necator in channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Ichthyobodo necator is a single celled biflagellate that can cause significant mortalities in fish, particularly young, tank-reared fish. Copper sulfate (CuSO4), potassium permanganate (KMnO4) and peracetic acid (PAA) were evaluated for effectiveness against Ichthybodosis in juvenile channel catfis...

  7. Growth and feed conversion of pond-raised hybrid catfish harvested at different sizes

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A study was conducted to examine growth and feed conversion ratio (FCR) of pond-raised hybrid catfish (channel catfish Ictalurus punctatus × blue catfish I. furcatus) harvested at different sizes. Fingerling hybrid catfish (mean initial weight ± SD = 62 ± 2.0 g/fish) were stocked into 25 earthen pon...

  8. Sulfonation of 17β-estradiol and inhibition of sulfotransferase activity by polychlorobiphenylols and celecoxib in Channel catfish, Ictalurus punctatus

    PubMed Central

    Wang, Li-Quan; James, Margaret O.

    2007-01-01

    The sulfonation of 17β-estradiol (E2) by human liver and recombinant sulfotransferases is influenced by environmental contaminants such as hydroxylated metabolites of polychlorinated biphenyls (OH-PCBs), which are potent inhibitors, and the therapeutic drug, celecoxib, which affects positional sulfonation of E2. In some locations, the aquatic environment is contaminated by PCBs, OH-PCBs and widely used therapeutic drugs. The objectives of this study were to investigate the sulfonation kinetics of E2 in liver cytosol from channel catfish (Ictalurus punctatus); to examine the effect of OH-PCBs on E2 sulfonation; and to determine if celecoxib altered the position of E2 sulfonation, as it does with human liver cytosol. E2 was converted to both 3- and 17-sulfates by catfish liver cytosol. At E2 concentrations below 1 μM, formation of E2-3-sulfate (E2-3-S) predominated, but substrate inhibition was observed at higher concentrations. Rates of E2-3-S formation at different E2 concentrations were fit to a substrate inhibition model, with K′m and V′max values of 0.40 ± 0.10 μM and 91.0 ± 4.7 pmol/min/mg protein, respectively and Ki of 1.08 ± 0.09 μM. The formation of E2-17-S fit Michaelis-Menten kinetics over the concentration range 25 nM to 2.5 μM, with Km and Vmax values of 1.07 ± 0.23 μM and 25.7 ± 4.43 pmol/min/mg protein, respectively. The efficiency (Vmax/Km) of formation of E2-3-S was 9.8-fold higher than that of E2-17-S. Several OH-PCBs inhibited E2 3-sulfonation, measured at an E2 concentration of 1 nM. Of those tested, the most potent inhibitor was 4′-OH-CB79, with two chlorine atoms flanking the OH group (IC50: 94 nM). The inhibition of estrogen sulfonation by OH-PCBs may disrupt the endocrine system and thus contribute to the known toxic effects of these compounds. Celecoxib did not stimulate E2-17-S formation, as is the case with human liver cytosol, but did inhibit the formation of E2-3-S (IC50: 44 μM) and to a lesser extent, E2-17-S (IC50

  9. Genetic variation in feed consumption, growth, nutrient utilization efficiency and mitochondrial function within a farmed population of channel catfish (Ictalurus punctatus).

    PubMed

    Eya, Jonathan C; Ashame, Martha F; Pomeroy, Charles F; Manning, Bruce B; Peterson, Brian C

    2012-10-01

    We evaluated the effects of diets (32/4 or 36/6 percent protein/fat) and six channel catfish families for growth performance characteristics. Two families with fast- (C) and slow- (D) growth rate and with low and high feed efficiency (FE) were selected for analyses of mitochondrial complex enzymatic activities (I, II, III, and IV) and gene expression (ND1, CYTB, COX1, COX2, ATP6) levels in liver, muscle, and intestine. There were significant differences in growth rate and nutrient retention among the families. Mitochondrial enzymatic complex activities (I-V) in the tissues were all lower in family C. Four of the five genes were down-regulated in the liver and up-regulated in the muscle for the fast growing family C. There were significant differences between diets for some mitochondrial respiratory chain enzyme activities and gene expression levels. Significant diet×family interactions were observed for some enzyme activities and gene expression levels. Changes in mitochondrial respiratory chain enzyme activities and gene expression levels provide insight into the cellular mechanisms of fish with differences in growth rate and feed efficiency. Results also suggest that genotype×diet interactions should be accounted for when considering strategies for using mitochondrial function as a criteria in channel catfish selection programs for improved growth performance characteristics. PMID:22691874

  10. Effects of calcium and magnesium hardness on the fertilization and hatching success of channel X blue hybrid catfish eggs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The aquifer used for hybrid catfish hatcheries is less than 10 mg/L of calcium hardness and 1- 25 mg/L of magnesium hardness. Embryonic development is deemed to be the most sensitive stage in the life cycle of a teleost. As egg development takes outside the fish’s body, water hardness is one abioti...

  11. An in vitro screening method to evaluate chemicals as potential chemotherapeutants to control Aeromonas hydrophila infection in channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Using catfish gill cells G1B and four chemicals (hydrogen peroxide, sodium chloride, potassium permanganate, and D-mannose), the feasibility of using an in vitro screening method to identify potential effective chemotherapeutants was evaluated in this study. In vitro screening results revealed that,...

  12. Effect of microbial transglutaminase and non-meat proteins on functional properties of low NaCl, phosphate-free patties made from channel catfish (Ictalurus punctatus) belly flap meat

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study was focused on developing value-added low sodium chloride (NaCl), phosphate-free restructured patties using minced channel catfish (Ictalurus punctatus) belly flap meat. The effect of microbial transglutaminase (MTGase, 0.7%) and non-meat proteins (isolated soy protein, ISP and whey prote...

  13. Growth performance, and proximate and fatty acid composition of channel catfish, Ictalurus punctatus, fed for different duration with a commercial diet supplemented with various levels of menhaden fish oil

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study was conducted to evaluate the effect of supplemental menhaden fish oil at levels of 0, 3, 6 and 9% to a commercial diet and feeding duration on growth performance, proximate body and tissue fatty acid (FA) composition of juvenile channel catfish, Ictalurus punctatus. Each diet was fed to ...

  14. Effect of microbial transglutaminase and non-meat proteins on functional properties of low NaCl, phosphate-free patties made from channel catfish (Ictalurus punctatus) belly flap meat

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study was aimed at developing value-added low sodium chloride (NaCl), phosphate-free restructured patties using minced channel catfish (Ictalurus punctatus) belly flap meat. The effect of microbial transglutaminase (MTGase, 0.7%) and non-meat proteins (isolated soy protein, ISP and whey protein...

  15. Effect of short-term feeding duration of diets containing commercial whole-cell yeast or yeast subcomponents on immune function and disease resistance in channel catfish, Ictalurus punctatus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Juvenile channel catfish (14.4 g average initial weight) were fed diets supplemented with a purified nucleotide mixture for 8 weeks. The mixture consisted of five nucleotides supplied on an equal basis as disodium salts at combined concentrations of 0 (control), 0.1, 0.3, 0.9, or 2.7% of diet. At th...

  16. Use of microbial transglutaminase and non-meat proteins to improve functional properties of low NaCl, phosphate-free patties made from channel catfish (Ictalurus punctatus) belly flap meat

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study was aimed at developing a value-added low sodium chloride (NaCl), phosphate-free patty using minced channel catfish (Ictalurus punctatus) belly flap meat. The effect of microbial transglutaminase (MTGase) and non-meat proteins (isolated soy protein, ISP and whey protein concentrate, WPC) ...

  17. The management of undesirable cyanobacteria blooms in channel catfish ponds using a constructed wetland: Contribution to the control of off-flavor occurrences.

    PubMed

    Zhong, Fei; Gao, Yunni; Yu, Tao; Zhang, Yongyuan; Xu, Dong; Xiao, Enrong; He, Feng; Zhou, Qiaohong; Wu, Zhenbin

    2011-12-01

    An exploratory study on the management of undesirable cyanobacteria blooms with respect to off-flavor problems using an integrated vertical-flow constructed wetland (CW) was performed at a small commercial-scale channel catfish farm from 2004 to 2007. The results of the three-year experiment indicated that water treatment by the CW could reduce the possibility of dominance by undesirable cyanobacteria species that often cause off-flavor problems. A detailed investigation in 2007, showed that the concentrations of geosmin, MIB (2-methylisoborneol), and β-cyclocitral in the water of the recirculating pond (4.3ngL(-1), U.D. (undetected) and 0.2ngL(-1), respectively) treated by the CW were significantly lower than those in the control pond (152.6ngL(-1), 63.3ngL(-1) and 254.8ngL(-1), respectively). In addition, the relationships among the cyanobacteria species, the off-flavor compounds and ten environmental variables were explored by canonical correspondence analysis (CCA). The results showed that Oscillatoria sp., Oscillatoria kawamurae and Microcystis aeruginosa were the main sources of off-flavor compounds in the catfish ponds. The successful manipulation of undesirable cyanobacteria species potentially resulted in lower concentrations of odorous compounds in the water of the recirculating pond. An investigation of the concentrations of geosmin and MIB in catfish fillets showed that the levels of odorous compounds were below the OTC (odor threshold concentration) values in the recirculating pond but were above the OTC values from July to October in the control pond. Water recycling by the CW could potentially be one of the best management practices to control off-flavor occurrences in aquaculture. PMID:22000715

  18. Zero-inflated modeling of fish catch per unit area resulting from multiple gears: Application to channel catfish and shovelnose sturgeon in the Missouri River

    USGS Publications Warehouse

    Arab, A.; Wildhaber, M.L.; Wikle, C.K.; Gentry, C.N.

    2008-01-01

    Fisheries studies often employ multiple gears that result in large percentages of zero values. We considered a zero-inflated Poisson (ZIP) model with random effects to address these excessive zeros. By employing a Bayesian ZIP model that simultaneously incorporates data from multiple gears to analyze data from the Missouri River, we were able to compare gears and make more year, segment, and macrohabitat comparisons than did the original data analysis. For channel catfish Ictalurus punctatus, our results rank (highest to lowest) the mean catch per unit area (CPUA) for gears (beach seine, benthic trawl, electrofishing, and drifting trammel net); years (1998 and 1997); macrohabitats (tributary mouth, connected secondary channel, nonconnected secondary channel, and bend); and river segment zones (channelized, inter-reservoir, and least-altered). For shovelnose sturgeon Scaphirhynchus platorynchus, the mean CPUA was significantly higher for benthic trawls and drifting trammel nets; 1998 and 1997; tributary mouths, bends, and connected secondary channels; and some channelized or least-altered inter-reservoir segments. One important advantage of our approach is the ability to reliably infer patterns of relative abundance by means of multiple gears without using gear efficiencies. ?? Copyright by the American Fisheries Society 2008.

  19. Immune responses and host protection of channel catfish, Ictalurus punctatus (Rafinesque), against Ichthyophthirius multifiliis after immunization with live theronts and sonicated trophonts.

    PubMed

    Xu, D-H; Klesius, P H; Shelby, R A

    2004-03-01

    The humoral immune responses and host protection of channel catfish, Ictalurus punctatus (Rafinesque), against Ichthyophthirius multifiliis (Ich) were determined after immunization with live theronts and sonicated trophonts. Immunizations with live theronts or sonicated trophonts were carried out by both bath immersion and intraperitoneal (i.p.) injection. Cutaneous and serum immunoglobulin (Ig) levels and anti-Ich antibodies were measured 12 and 21 days post-immunization. The level of Ich infection and survival of catfish were determined after theront challenge. Cutaneous and serum anti-Ich antibodies were significantly higher (P < 0.05) in fish immunized with live theronts by immersion or i.p. injection, or with sonicated trophonts administered by i.p. injection, than in fish immunized with sonicated trophonts by immersion, with bovine serum albumin by i.p. injection, or non-immunized controls. Host protection was noted only in fish immunized with live theronts by immersion or i.p. injection or with sonicated trophonts by i.p. injection. There was a positive correlation between higher levels of anti-Ich antibodies and host survival in the immunized fish. PMID:15009239

  20. Interaction of xenobiotics with estrogen receptors α and β and a putative plasma sex hormone-binding globulin from channel catfish (Ictalurus punctatus)

    USGS Publications Warehouse

    Gale, William L.; Patino, Reynaldo; Maule, Alec G.

    2004-01-01

    Estrogens are important regulators of physiological functions. Although environmental contaminants (xenoestrogens) which interfere with estrogen signaling are of increasing concern, there is only limited information about their ability to interact with estrogen-binding proteins (SHBG) or receptors (ER). Recombinant ER?? and ?? were obtained after transient transfection of COS-7 cells with channel catfish ER cDNA. Plasma from adult female channel catfish was the source of SHBG. Tritiated estradiol ( 3H-E2) was used in standard radioligand-binding assays to characterize the binding properties of channel catfish SHBG (ccfSHBG) and to estimate the inhibition constants for various estrogenic compounds. Binding of 3H-E2 to ccfSHBG was saturable and of high affinity with a Kd (??SE) of 1.9??0.14nM and a Bmax of 14.3??2.4pmol/mg protein (n=3 assays). Additionally, ccfSHBG displayed binding specificity for androgens and estrogens. Endosulfan, 4-nonylphenol, and 4-octylphenol displaced 3H-E2 binding to ccfSHBG albeit only at very high concentrations, whereas dieldrin and atrazine showed little displacement activity even at the highest concentrations used. The synthetic estrogen ethynylestradiol had higher affinity than E2 for ccfSHBG. This finding differs from results with human and rainbow trout SHBG. The alkylphenolic compounds (4-octylphenol and 4-nonylphenol) displayed some ability to displace 3H-E2 binding from ER?? and ?? at high concentrations, but dieldrin and atrazine had little binding activity for both ER subtypes and endosulfan for ER??. The xenobiotics tested generally showed equivalent or greater affinity for ER?? than ER??, whereas natural estrogens had much greater affinity for ER?? than ER??. These observations suggest that results of studies using fish tissue ER extracts must be interpreted with caution, since both ER subtypes may be present, and that the binding of xenoestrogens to SHBG must be taken into account for proper assessment of endocrine