Science.gov

Sample records for agency designates specific

  1. 29 CFR 2703.2 - Designated agency ethics official and alternate designated agency ethics official.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 9 2014-07-01 2014-07-01 false Designated agency ethics official and alternate designated agency ethics official. 2703.2 Section 2703.2 Labor Regulations Relating to Labor (Continued) FEDERAL... agency ethics official and alternate designated agency ethics official. The Chairman shall appoint...

  2. 29 CFR 2703.2 - Designated agency ethics official and alternate designated agency ethics official.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 9 2011-07-01 2011-07-01 false Designated agency ethics official and alternate designated agency ethics official. 2703.2 Section 2703.2 Labor Regulations Relating to Labor (Continued) FEDERAL... agency ethics official and alternate designated agency ethics official. The Chairman shall appoint...

  3. 29 CFR 2703.2 - Designated agency ethics official and alternate designated agency ethics official.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Designated agency ethics official and alternate designated agency ethics official. 2703.2 Section 2703.2 Labor Regulations Relating to Labor (Continued) FEDERAL... agency ethics official and alternate designated agency ethics official. The Chairman shall appoint...

  4. 29 CFR 2703.2 - Designated agency ethics official and alternate designated agency ethics official.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 9 2013-07-01 2013-07-01 false Designated agency ethics official and alternate designated agency ethics official. 2703.2 Section 2703.2 Labor Regulations Relating to Labor (Continued) FEDERAL... agency ethics official and alternate designated agency ethics official. The Chairman shall appoint...

  5. 29 CFR 2703.2 - Designated agency ethics official and alternate designated agency ethics official.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 9 2012-07-01 2012-07-01 false Designated agency ethics official and alternate designated agency ethics official. 2703.2 Section 2703.2 Labor Regulations Relating to Labor (Continued) FEDERAL... agency ethics official and alternate designated agency ethics official. The Chairman shall appoint...

  6. 31 CFR 0.104 - Designated Agency Ethics Official and Alternate Designated Agency Ethics Official.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Standards and Treasury Supplemental Standards and Rules. See 5 CFR 2638.203. The Senior Counsel for Ethics... 31 Money and Finance: Treasury 1 2014-07-01 2014-07-01 false Designated Agency Ethics Official and Alternate Designated Agency Ethics Official. 0.104 Section 0.104 Money and Finance: Treasury Office of...

  7. 31 CFR 0.104 - Designated Agency Ethics Official and Alternate Designated Agency Ethics Official.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Standards and Treasury Supplemental Standards and Rules. See 5 CFR 2638.203. The Senior Counsel for Ethics... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Designated Agency Ethics Official and Alternate Designated Agency Ethics Official. 0.104 Section 0.104 Money and Finance: Treasury Office of...

  8. 31 CFR 0.104 - Designated Agency Ethics Official and Alternate Designated Agency Ethics Official.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Standards and Treasury Supplemental Standards and Rules. See 5 CFR 2638.203. The Senior Counsel for Ethics... 31 Money and Finance: Treasury 1 2011-07-01 2011-07-01 false Designated Agency Ethics Official and Alternate Designated Agency Ethics Official. 0.104 Section 0.104 Money and Finance: Treasury Office of...

  9. 31 CFR 0.104 - Designated Agency Ethics Official and Alternate Designated Agency Ethics Official.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Standards and Treasury Supplemental Standards and Rules. See 5 CFR 2638.203. The Senior Counsel for Ethics... 31 Money and Finance: Treasury 1 2013-07-01 2013-07-01 false Designated Agency Ethics Official and Alternate Designated Agency Ethics Official. 0.104 Section 0.104 Money and Finance: Treasury Office of...

  10. 31 CFR 0.104 - Designated Agency Ethics Official and Alternate Designated Agency Ethics Official.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Standards and Treasury Supplemental Standards and Rules. See 5 CFR 2638.203. The Senior Counsel for Ethics... 31 Money and Finance: Treasury 1 2012-07-01 2012-07-01 false Designated Agency Ethics Official and Alternate Designated Agency Ethics Official. 0.104 Section 0.104 Money and Finance: Treasury Office of...

  11. 34 CFR 303.142 - Designation of lead agency.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Designation of lead agency. 303.142 Section 303.142 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION....142 Designation of lead agency. Each application must include a designation of the lead agency in...

  12. 34 CFR 303.142 - Designation of lead agency.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Designation of lead agency. 303.142 Section 303.142 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION....142 Designation of lead agency. Each application must include a designation of the lead agency in...

  13. 49 CFR 659.9 - Designation of oversight agency.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... state, the state must designate an agency of the state, other than the rail transit agency, as the... project between FTA and a rail transit agency within the state's jurisdiction; or (2) Occur before the application by a rail transit agency for funding under FTA's formula program for urbanized areas (49...

  14. 49 CFR 659.9 - Designation of oversight agency.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... state, the state must designate an agency of the state, other than the rail transit agency, as the... project between FTA and a rail transit agency within the state's jurisdiction; or (2) Occur before the application by a rail transit agency for funding under FTA's formula program for urbanized areas (49...

  15. 49 CFR 659.9 - Designation of oversight agency.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... state, the state must designate an agency of the state, other than the rail transit agency, as the... project between FTA and a rail transit agency within the state's jurisdiction; or (2) Occur before the application by a rail transit agency for funding under FTA's formula program for urbanized areas (49...

  16. 49 CFR 659.9 - Designation of oversight agency.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... state, the state must designate an agency of the state, other than the rail transit agency, as the... project between FTA and a rail transit agency within the state's jurisdiction; or (2) Occur before the application by a rail transit agency for funding under FTA's formula program for urbanized areas (49...

  17. 49 CFR 659.9 - Designation of oversight agency.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... state, the state must designate an agency of the state, other than the rail transit agency, as the... project between FTA and a rail transit agency within the state's jurisdiction; or (2) Occur before the application by a rail transit agency for funding under FTA's formula program for urbanized areas (49...

  18. 13 CFR 105.403 - Designated Agency Ethics Officials.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Designated Agency Ethics Officials. 105.403 Section 105.403 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION STANDARDS OF... Agency Ethics Officials. The Designated Agency Ethics Official and Alternates administer the program...

  19. 13 CFR 105.403 - Designated Agency Ethics Officials.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Designated Agency Ethics Officials. 105.403 Section 105.403 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION STANDARDS OF... Agency Ethics Officials. The Designated Agency Ethics Official and Alternates administer the program...

  20. 13 CFR 105.403 - Designated Agency Ethics Officials.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Designated Agency Ethics Officials. 105.403 Section 105.403 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION STANDARDS OF... Agency Ethics Officials. The Designated Agency Ethics Official and Alternates administer the program...

  1. 13 CFR 105.403 - Designated Agency Ethics Officials.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Designated Agency Ethics Officials. 105.403 Section 105.403 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION STANDARDS OF... Agency Ethics Officials. The Designated Agency Ethics Official and Alternates administer the program...

  2. 13 CFR 105.403 - Designated Agency Ethics Officials.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Designated Agency Ethics Officials. 105.403 Section 105.403 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION STANDARDS OF... Agency Ethics Officials. The Designated Agency Ethics Official and Alternates administer the program...

  3. 34 CFR 303.201 - Designation of lead agency.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false Designation of lead agency. 303.201 Section 303.201 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION... of lead agency. Each application must include the name of the State lead agency, as designated...

  4. 50 CFR 402.07 - Designation of lead agency.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Designation of lead agency. 402.07 Section... COOPERATION-ENDANGERED SPECIES ACT OF 1973, AS AMENDED General § 402.07 Designation of lead agency. When a... be fulfilled through a lead agency. Factors relevant in determining an appropriate lead...

  5. 50 CFR 402.07 - Designation of lead agency.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Designation of lead agency. 402.07 Section... COOPERATION-ENDANGERED SPECIES ACT OF 1973, AS AMENDED General § 402.07 Designation of lead agency. When a... be fulfilled through a lead agency. Factors relevant in determining an appropriate lead...

  6. 43 CFR 46.220 - How to designate lead agencies.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... the non-Federal agency as a joint lead agency. (See 40 CFR 1501.5 and 1506.2 for a description of the... 43 Public Lands: Interior 1 2012-10-01 2011-10-01 true How to designate lead agencies. 46.220... NATIONAL ENVIRONMENTAL POLICY ACT OF 1969 Initiating the NEPA Process § 46.220 How to designate...

  7. 50 CFR 402.07 - Designation of lead agency.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 11 2012-10-01 2012-10-01 false Designation of lead agency. 402.07... COOPERATION-ENDANGERED SPECIES ACT OF 1973, AS AMENDED General § 402.07 Designation of lead agency. When a... be fulfilled through a lead agency. Factors relevant in determining an appropriate lead...

  8. 34 CFR 303.500 - Lead agency establishment or designation.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Lead agency establishment or designation. 303.500... INFANTS AND TODDLERS WITH DISABILITIES State Administration General § 303.500 Lead agency establishment or designation. Each system must include a single line of responsibility in a lead agency that— (a)...

  9. 50 CFR 402.07 - Designation of lead agency.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 11 2014-10-01 2014-10-01 false Designation of lead agency. 402.07... COOPERATION-ENDANGERED SPECIES ACT OF 1973, AS AMENDED General § 402.07 Designation of lead agency. When a... be fulfilled through a lead agency. Factors relevant in determining an appropriate lead...

  10. 34 CFR 303.201 - Designation of lead agency.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Designation of lead agency. 303.201 Section 303.201 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION... of lead agency. Each application must include the name of the State lead agency, as designated...

  11. 43 CFR 46.220 - How to designate lead agencies.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... the non-Federal agency as a joint lead agency. (See 40 CFR 1501.5 and 1506.2 for a description of the... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false How to designate lead agencies. 46.220... NATIONAL ENVIRONMENTAL POLICY ACT OF 1969 Initiating the NEPA Process § 46.220 How to designate...

  12. 50 CFR 402.07 - Designation of lead agency.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Designation of lead agency. 402.07... COOPERATION-ENDANGERED SPECIES ACT OF 1973, AS AMENDED General § 402.07 Designation of lead agency. When a... be fulfilled through a lead agency. Factors relevant in determining an appropriate lead...

  13. 43 CFR 46.220 - How to designate lead agencies.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... the non-Federal agency as a joint lead agency. (See 40 CFR 1501.5 and 1506.2 for a description of the... 43 Public Lands: Interior 1 2013-10-01 2013-10-01 false How to designate lead agencies. 46.220... NATIONAL ENVIRONMENTAL POLICY ACT OF 1969 Initiating the NEPA Process § 46.220 How to designate...

  14. 34 CFR 303.500 - Lead agency establishment or designation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Lead agency establishment or designation. 303.500... INFANTS AND TODDLERS WITH DISABILITIES State Administration General § 303.500 Lead agency establishment or designation. Each system must include a single line of responsibility in a lead agency that— (a)...

  15. 43 CFR 46.220 - How to designate lead agencies.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... the non-Federal agency as a joint lead agency. (See 40 CFR 1501.5 and 1506.2 for a description of the... 43 Public Lands: Interior 1 2011-10-01 2011-10-01 false How to designate lead agencies. 46.220... NATIONAL ENVIRONMENTAL POLICY ACT OF 1969 Initiating the NEPA Process § 46.220 How to designate...

  16. 43 CFR 46.220 - How to designate lead agencies.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... the non-Federal agency as a joint lead agency. (See 40 CFR 1501.5 and 1506.2 for a description of the... 43 Public Lands: Interior 1 2014-10-01 2014-10-01 false How to designate lead agencies. 46.220... NATIONAL ENVIRONMENTAL POLICY ACT OF 1969 Initiating the NEPA Process § 46.220 How to designate...

  17. 34 CFR 303.201 - Designation of lead agency.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true Designation of lead agency. 303.201 Section 303.201 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION... of lead agency. Each application must include the name of the State lead agency, as designated...

  18. Reflight certification software design specifications

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The PDSS/IMC Software Design Specification for the Payload Development Support System (PDSS)/Image Motion Compensator (IMC) is contained. The PDSS/IMC is to be used for checkout and verification of the IMC flight hardware and software by NASA/MSFC.

  19. Addressing dual agency: getting specific about the expectations of professionalism.

    PubMed

    Tilburt, Jon C

    2014-01-01

    Professionalism requires that physicians uphold the best interests of patients while simultaneously insuring just use of health care resources. Current articulations of these obligations like the American Board of Internal Medicine (ABIM) Foundation's Physician Charter do not reconcile how these obligations fit together when they conflict. This is the problem of dual agency. The most common ways of dealing with dual agency: "bunkering"--physicians act as though societal cost issues are not their problem; "bailing"--physicians assume that they are merely agents of society and deliver care typically based on a strongly consequentialist public health ethic; or "balancing"--a vaguely specified attempt to uphold both patient welfare and societal need for judicious resource use simultaneously--all fail. Here I propose how the problem of dual agency might begin to be addressed with rigor and consistency. Without dealing with the dual agency problem and getting more specific about how to reconcile its norms when they conflict, the expectations of professionalism risk being written off as cute, nonbinding aphorisms from the medical profession. PMID:25127273

  20. Addressing dual agency: getting specific about the expectations of professionalism.

    PubMed

    Tilburt, Jon C

    2014-01-01

    Professionalism requires that physicians uphold the best interests of patients while simultaneously insuring just use of health care resources. Current articulations of these obligations like the American Board of Internal Medicine (ABIM) Foundation's Physician Charter do not reconcile how these obligations fit together when they conflict. This is the problem of dual agency. The most common ways of dealing with dual agency: "bunkering"--physicians act as though societal cost issues are not their problem; "bailing"--physicians assume that they are merely agents of society and deliver care typically based on a strongly consequentialist public health ethic; or "balancing"--a vaguely specified attempt to uphold both patient welfare and societal need for judicious resource use simultaneously--all fail. Here I propose how the problem of dual agency might begin to be addressed with rigor and consistency. Without dealing with the dual agency problem and getting more specific about how to reconcile its norms when they conflict, the expectations of professionalism risk being written off as cute, nonbinding aphorisms from the medical profession.

  1. 45 CFR 1321.33 - Designation of area agencies.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.33 Designation of... area agency on aging is designated, the State shall give right of first refusal to a unit of...

  2. 45 CFR 1321.33 - Designation of area agencies.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.33 Designation of... area agency on aging is designated, the State shall give right of first refusal to a unit of...

  3. 45 CFR 1321.33 - Designation of area agencies.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.33 Designation of... area agency on aging is designated, the State shall give right of first refusal to a unit of...

  4. 45 CFR 1321.33 - Designation of area agencies.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.33 Designation of... area agency on aging is designated, the State shall give right of first refusal to a unit of...

  5. 45 CFR 1321.33 - Designation of area agencies.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.33 Designation of... area agency on aging is designated, the State shall give right of first refusal to a unit of...

  6. 28 CFR 81.4 - Referral of reports where the designated agency is not a law enforcement agency.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.4 Referral of reports where the designated agency is not a law enforcement agency. Where a report of child abuse received by a designated agency that is not a law enforcement agency involves allegations of sexual...

  7. 28 CFR 35.190 - Designated agencies.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... local government components, and shall provide policy guidance and interpretations to designated... institutions, institutions of higher education and vocational education (other than schools of...

  8. 28 CFR 35.190 - Designated agencies.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... local government components, and shall provide policy guidance and interpretations to designated... institutions, institutions of higher education and vocational education (other than schools of...

  9. 5 CFR 6001.103 - Designation of separate agency components.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... separate agency components. (a) Pursuant to 5 CFR 2635.203(a), each of the following components of the... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Designation of separate agency components... of 5 CFR part 2635 governing gifts from outside sources and § 2635.807 of this title...

  10. Smile design--specific considerations.

    PubMed

    Morley, J

    1997-09-01

    Smile design is a new discipline that has come to the forefront with the recent popularity of cosmetic dentistry techniques. This article explores some of the principles used in smile design that can enhance the esthetics of any anterior restorative procedure.

  11. 78 FR 33863 - Relationship Between General Design Criteria and Technical Specification Operability

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-05

    ... this RIS in the Federal Register (77 FR 45282) on July 31, 2012. The agency received comments from two... COMMISSION Relationship Between General Design Criteria and Technical Specification Operability AGENCY... Relationship Between General Design Criteria and Technical Specification Operability.'' This RIS clarifies...

  12. 28 CFR 81.4 - Referral of reports where the designated agency is not a law enforcement agency.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.4 Referral of reports where the designated agency is not a law enforcement agency. Where a report of child abuse..., serious physical injury, or life-threatening neglect of a child, that agency shall immediately report...

  13. 28 CFR 81.4 - Referral of reports where the designated agency is not a law enforcement agency.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.4 Referral of reports where the designated agency is not a law enforcement agency. Where a report of child abuse..., serious physical injury, or life-threatening neglect of a child, that agency shall immediately report...

  14. 28 CFR 81.4 - Referral of reports where the designated agency is not a law enforcement agency.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.4 Referral of reports where the designated agency is not a law enforcement agency. Where a report of child abuse..., serious physical injury, or life-threatening neglect of a child, that agency shall immediately report...

  15. 28 CFR 81.4 - Referral of reports where the designated agency is not a law enforcement agency.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.4 Referral of reports where the designated agency is not a law enforcement agency. Where a report of child abuse..., serious physical injury, or life-threatening neglect of a child, that agency shall immediately report...

  16. 5 CFR 3601.102 - Designation of separate agency components.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... outside sources and 5 CFR 2635.807 governing teaching, speaking and writing: (1) Armed Services Board of... identifying the DoD employee's agency under 5 CFR 2635.807 governing teaching, speaking and writing. ... components. (a) Pursuant to 5 CFR 2635.203(a), each of the following components of DoD is designated as...

  17. 5 CFR 3601.102 - Designation of separate agency components.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... outside sources and 5 CFR 2635.807 governing teaching, speaking and writing: (1) Armed Services Board of... identifying the DoD employee's agency under 5 CFR 2635.807 governing teaching, speaking and writing. ... components. (a) Pursuant to 5 CFR 2635.203(a), each of the following components of DoD is designated as...

  18. 5 CFR 3101.102 - Designation of separate agency components.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... components. Pursuant to 5 CFR 2635.203(a), each of the following components of the Department of the Treasury is designated as a separate agency for purposes of the regulations contained in subpart B of 5 CFR part 2635 governing gifts from outside sources and 5 CFR 2635.807 governing teaching, speaking...

  19. 5 CFR 3101.102 - Designation of separate agency components.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... components. Pursuant to 5 CFR 2635.203(a), each of the following components of the Department of the Treasury is designated as a separate agency for purposes of the regulations contained in subpart B of 5 CFR part 2635 governing gifts from outside sources and 5 CFR 2635.807 governing teaching, speaking...

  20. 5 CFR 3101.102 - Designation of separate agency components.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... components. Pursuant to 5 CFR 2635.203(a), each of the following components of the Department of the Treasury is designated as a separate agency for purposes of the regulations contained in subpart B of 5 CFR part 2635 governing gifts from outside sources and 5 CFR 2635.807 governing teaching, speaking...

  1. 5 CFR 3101.102 - Designation of separate agency components.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... components. Pursuant to 5 CFR 2635.203(a), each of the following components of the Department of the Treasury is designated as a separate agency for purposes of the regulations contained in subpart B of 5 CFR part 2635 governing gifts from outside sources and 5 CFR 2635.807 governing teaching, speaking...

  2. 5 CFR 3101.102 - Designation of separate agency components.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... components. Pursuant to 5 CFR 2635.203(a), each of the following components of the Department of the Treasury is designated as a separate agency for purposes of the regulations contained in subpart B of 5 CFR part 2635 governing gifts from outside sources and 5 CFR 2635.807 governing teaching, speaking...

  3. 21 CFR 3.4 - Designated agency component.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 1 2012-04-01 2012-04-01 false Designated agency component. 3.4 Section 3.4 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PRODUCT... safety and effectiveness with regard to the combination product as a whole. When there are no...

  4. 21 CFR 3.4 - Designated agency component.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 1 2014-04-01 2014-04-01 false Designated agency component. 3.4 Section 3.4 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PRODUCT... safety and effectiveness with regard to the combination product as a whole. When there are no...

  5. 24 CFR 58.57 - Lead agency designation.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... responsible entity must make its decision based on the criteria in 40 CFR 1501.5(c). If the responsible entity... procedure set forth in 40 CFR 1501.5(e). ... 24 Housing and Urban Development 1 2013-04-01 2013-04-01 false Lead agency designation....

  6. 24 CFR 58.57 - Lead agency designation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... responsible entity must make its decision based on the criteria in 40 CFR 1501.5(c). If the responsible entity... procedure set forth in 40 CFR 1501.5(e). ... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Lead agency designation....

  7. 24 CFR 58.57 - Lead agency designation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... responsible entity must make its decision based on the criteria in 40 CFR 1501.5(c). If the responsible entity... procedure set forth in 40 CFR 1501.5(e). ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Lead agency designation....

  8. 24 CFR 58.57 - Lead agency designation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... responsible entity must make its decision based on the criteria in 40 CFR 1501.5(c). If the responsible entity... procedure set forth in 40 CFR 1501.5(e). ... 24 Housing and Urban Development 1 2012-04-01 2012-04-01 false Lead agency designation....

  9. 24 CFR 58.57 - Lead agency designation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... responsible entity must make its decision based on the criteria in 40 CFR 1501.5(c). If the responsible entity... procedure set forth in 40 CFR 1501.5(e). ... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false Lead agency designation....

  10. 78 FR 28258 - mPower\\TM\\ Design-Specific Review Standard

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-14

    ... COMMISSION mPower\\TM\\ Design-Specific Review Standard AGENCY: Nuclear Regulatory Commission. ACTION: Design-Specific Review Standard (DSRS) for the mPower\\TM\\ Design; request for comment. SUMMARY: The U.S. Nuclear... the mPower\\TM\\ design (mPower\\TM\\ DSRS). The purpose of the mPower\\TM\\ DSRS is to more fully...

  11. Transitioning from conceptual design to construction performance specification

    NASA Astrophysics Data System (ADS)

    Jeffers, Paul; Warner, Mark; Craig, Simon; Hubbard, Robert; Marshall, Heather

    2012-09-01

    On successful completion of a conceptual design review by a funding agency or customer, there is a transition phase before construction contracts can be placed. The nature of this transition phase depends on the Project's approach to construction and the particular subsystem being considered. There are generically two approaches; project retention of design authority and issuance of build to print contracts, or issuance of subsystem performance specifications with controlled interfaces. This paper relates to the latter where a proof of concept (conceptual or reference design) is translated into performance based sub-system specifications for competitive tender. This translation is not a straightforward process and there are a number of different issues to consider in the process. This paper deals with primarily the Telescope mount and Enclosure subsystems. The main subjects considered in this paper are: • Typical status of design at Conceptual Design Review compared with the desired status of Specifications and Interface Control Documents at Request for Quotation. • Options for capture and tracking of system requirements flow down from science / operating requirements and sub-system requirements, and functional requirements derived from reference design. • Requirements that may come specifically from the contracting approach. • Methods for effective use of reference design work without compromising a performance based specification. • Management of project team's expectation relating to design. • Effects on cost estimates from reference design to actual. This paper is based on experience and lessons learned through this process on both the VISTA and the ATST projects.

  12. 34 CFR 370.41 - What conflict of interest provision applies to employees of a designated agency?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... other rehabilitation project, program, or community rehabilitation program receiving assistance under... policymaking and administrative personnel in State and local rehabilitation programs, projects, and community rehabilitation programs, if consultation with the designated agency is specifically permitted or mandated by...

  13. Engineered waste-package-system design specification

    SciTech Connect

    Not Available

    1983-05-01

    This report documents the waste package performance requirements and geologic and waste form data bases used in developing the conceptual designs for waste packages for salt, tuff, and basalt geologies. The data base reflects the latest geotechnical information on the geologic media of interest. The parameters or characteristics specified primarily cover spent fuel, defense high-level waste, and commercial high-level waste forms. The specification documents the direction taken during the conceptual design activity. A separate design specification will be developed prior to the start of the preliminary design activity.

  14. 78 FR 52804 - mPower\\TM\\ Design-Specific Review Standard

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-26

    ... revision of the Standard Review Plan. C. Re-Opening of Comment Period On May 14, 2013 (78 FR 28258), the... COMMISSION mPower\\TM\\ Design-Specific Review Standard AGENCY: Nuclear Regulatory Commission. ACTION: Design-Specific Review Standard (DSRS) for the mPower\\TM\\ Design; re-opening of comment period. SUMMARY: On May...

  15. Formalisms for user interface specification and design

    NASA Technical Reports Server (NTRS)

    Auernheimer, Brent J.

    1989-01-01

    The application of formal methods to the specification and design of human-computer interfaces is described. A broad outline of human-computer interface problems, a description of the field of cognitive engineering and two relevant research results, the appropriateness of formal specification techniques, and potential NASA application areas are described.

  16. URPD: a specific product primer design tool

    PubMed Central

    2012-01-01

    Background Polymerase chain reaction (PCR) plays an important role in molecular biology. Primer design fundamentally determines its results. Here, we present a currently available software that is not located in analyzing large sequence but used for a rather straight-forward way of visualizing the primer design process for infrequent users. Findings URPD (yoUR Primer Design), a web-based specific product primer design tool, combines the NCBI Reference Sequences (RefSeq), UCSC In-Silico PCR, memetic algorithm (MA) and genetic algorithm (GA) primer design methods to obtain specific primer sets. A friendly user interface is accomplished by built-in parameter settings. The incorporated smooth pipeline operations effectively guide both occasional and advanced users. URPD contains an automated process, which produces feasible primer pairs that satisfy the specific needs of the experimental design with practical PCR amplifications. Visual virtual gel electrophoresis and in silico PCR provide a simulated PCR environment. The comparison of Practical gel electrophoresis comparison to virtual gel electrophoresis facilitates and verifies the PCR experiment. Wet-laboratory validation proved that the system provides feasible primers. Conclusions URPD is a user-friendly tool that provides specific primer design results. The pipeline design path makes it easy to operate for beginners. URPD also provides a high throughput primer design function. Moreover, the advanced parameter settings assist sophisticated researchers in performing experiential PCR. Several novel functions, such as a nucleotide accession number template sequence input, local and global specificity estimation, primer pair redesign, user-interactive sequence scale selection, and virtual and practical PCR gel electrophoresis discrepancies have been developed and integrated into URPD. The URPD program is implemented in JAVA and freely available at http://bio.kuas.edu.tw/urpd/. PMID:22713312

  17. Domain specific software design for decision aiding

    NASA Technical Reports Server (NTRS)

    Keller, Kirby; Stanley, Kevin

    1992-01-01

    McDonnell Aircraft Company (MCAIR) is involved in many large multi-discipline design and development efforts of tactical aircraft. These involve a number of design disciplines that must be coordinated to produce an integrated design and a successful product. Our interpretation of a domain specific software design (DSSD) is that of a representation or framework that is specialized to support a limited problem domain. A DSSD is an abstract software design that is shaped by the problem characteristics. This parallels the theme of object-oriented analysis and design of letting the problem model directly drive the design. The DSSD concept extends the notion of software reusability to include representations or frameworks. It supports the entire software life cycle and specifically leads to improved prototyping capability, supports system integration, and promotes reuse of software designs and supporting frameworks. The example presented in this paper is the task network architecture or design which was developed for the MCAIR Pilot's Associate program. The task network concept supported both module development and system integration within the domain of operator decision aiding. It is presented as an instance where a software design exhibited many of the attributes associated with DSSD concept.

  18. 25 CFR 700.509 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... opinions shall be submitted as specified in 5 CFR 738.304. The Designated Agency Ethics Official shall... 25 Indians 2 2014-04-01 2014-04-01 false Duties of the designated agency ethics official. 700.509... RELOCATION PROCEDURES Employee Responsibility and Conduct § 700.509 Duties of the designated agency...

  19. 25 CFR 700.509 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... opinions shall be submitted as specified in 5 CFR 738.304. The Designated Agency Ethics Official shall... 25 Indians 2 2010-04-01 2010-04-01 false Duties of the designated agency ethics official. 700.509... RELOCATION PROCEDURES Employee Responsibility and Conduct § 700.509 Duties of the designated agency...

  20. 25 CFR 700.509 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... opinions shall be submitted as specified in 5 CFR 738.304. The Designated Agency Ethics Official shall... 25 Indians 2 2011-04-01 2011-04-01 false Duties of the designated agency ethics official. 700.509... RELOCATION PROCEDURES Employee Responsibility and Conduct § 700.509 Duties of the designated agency...

  1. 25 CFR 700.509 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... opinions shall be submitted as specified in 5 CFR 738.304. The Designated Agency Ethics Official shall... 25 Indians 2 2012-04-01 2012-04-01 false Duties of the designated agency ethics official. 700.509... RELOCATION PROCEDURES Employee Responsibility and Conduct § 700.509 Duties of the designated agency...

  2. 25 CFR 700.509 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... opinions shall be submitted as specified in 5 CFR 738.304. The Designated Agency Ethics Official shall... 25 Indians 2 2013-04-01 2013-04-01 false Duties of the designated agency ethics official. 700.509... RELOCATION PROCEDURES Employee Responsibility and Conduct § 700.509 Duties of the designated agency...

  3. 5 CFR 3601.102 - Designation of separate agency components.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Service; (8) Defense Information Systems Agency; (9) Defense Intelligence Agency; (10) Defense Logistics... separate agency for purposes of the regulations in subpart B of 5 CFR part 2635 governing gifts from... identifying the DoD employee's agency under 5 CFR 2635.807 governing teaching, speaking and writing....

  4. Learner Agency within the Design of an EAP Course

    ERIC Educational Resources Information Center

    Seppälä, Riina

    2015-01-01

    To meet the demands of today's society and working life, higher education should support the development of learner agency. How the agency of individual learners emerges in university courses and what kind of agency empowers the learners to face new challenges should be considered. In this article, the focus is on learner agency enabled and…

  5. 40 CFR 55.15 - Specific designation of corresponding onshore areas.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 5 2011-07-01 2011-07-01 false Specific designation of corresponding onshore areas. 55.15 Section 55.15 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) OUTER CONTINENTAL SHELF AIR REGULATIONS § 55.15 Specific designation...

  6. 45 CFR 1321.35 - Withdrawal of area agency designation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... DEVELOPMENT SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.35 Withdrawal... established and published by the State agency on aging; or (4) Activities of the area agency are...

  7. 45 CFR 1321.35 - Withdrawal of area agency designation.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... DEVELOPMENT SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.35 Withdrawal... established and published by the State agency on aging; or (4) Activities of the area agency are...

  8. 45 CFR 1321.35 - Withdrawal of area agency designation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... DEVELOPMENT SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.35 Withdrawal... established and published by the State agency on aging; or (4) Activities of the area agency are...

  9. 45 CFR 1321.35 - Withdrawal of area agency designation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... DEVELOPMENT SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.35 Withdrawal... established and published by the State agency on aging; or (4) Activities of the area agency are...

  10. 45 CFR 1321.35 - Withdrawal of area agency designation.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... DEVELOPMENT SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.35 Withdrawal... established and published by the State agency on aging; or (4) Activities of the area agency are...

  11. 5 CFR 2638.203 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Duties of the designated agency ethics official. 2638.203 Section 2638.203 Administrative Personnel OFFICE OF GOVERNMENT ETHICS GOVERNMENT ETHICS OFFICE OF GOVERNMENT ETHICS AND EXECUTIVE AGENCY ETHICS PROGRAM RESPONSIBILITIES Designated Agency...

  12. 5 CFR 2638.203 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 3 2011-01-01 2011-01-01 false Duties of the designated agency ethics official. 2638.203 Section 2638.203 Administrative Personnel OFFICE OF GOVERNMENT ETHICS GOVERNMENT ETHICS OFFICE OF GOVERNMENT ETHICS AND EXECUTIVE AGENCY ETHICS PROGRAM RESPONSIBILITIES Designated Agency...

  13. 5 CFR 2638.203 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Duties of the designated agency ethics official. 2638.203 Section 2638.203 Administrative Personnel OFFICE OF GOVERNMENT ETHICS GOVERNMENT ETHICS OFFICE OF GOVERNMENT ETHICS AND EXECUTIVE AGENCY ETHICS PROGRAM RESPONSIBILITIES Designated Agency...

  14. 5 CFR 2638.203 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 3 2012-01-01 2012-01-01 false Duties of the designated agency ethics official. 2638.203 Section 2638.203 Administrative Personnel OFFICE OF GOVERNMENT ETHICS GOVERNMENT ETHICS OFFICE OF GOVERNMENT ETHICS AND EXECUTIVE AGENCY ETHICS PROGRAM RESPONSIBILITIES Designated Agency...

  15. 40 CFR 68.215 - Permit content and air permitting authority or designated agency requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... authority or designated agency requirements. 68.215 Section 68.215 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) CHEMICAL ACCIDENT PREVENTION PROVISIONS Other... requested by the air permitting authority or designated agency. (c) For 40 CFR part 70 or part 71...

  16. 40 CFR 68.215 - Permit content and air permitting authority or designated agency requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... authority or designated agency requirements. 68.215 Section 68.215 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) CHEMICAL ACCIDENT PREVENTION PROVISIONS Other... requested by the air permitting authority or designated agency. (c) For 40 CFR part 70 or part 71...

  17. 40 CFR 68.215 - Permit content and air permitting authority or designated agency requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... authority or designated agency requirements. 68.215 Section 68.215 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) CHEMICAL ACCIDENT PREVENTION PROVISIONS Other... requested by the air permitting authority or designated agency. (c) For 40 CFR part 70 or part 71...

  18. 40 CFR 68.215 - Permit content and air permitting authority or designated agency requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... authority or designated agency requirements. 68.215 Section 68.215 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) CHEMICAL ACCIDENT PREVENTION PROVISIONS Other... requested by the air permitting authority or designated agency. (c) For 40 CFR part 70 or part 71...

  19. 40 CFR 68.215 - Permit content and air permitting authority or designated agency requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... authority or designated agency requirements. 68.215 Section 68.215 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) CHEMICAL ACCIDENT PREVENTION PROVISIONS Other... requested by the air permitting authority or designated agency. (c) For 40 CFR part 70 or part 71...

  20. 36 CFR 907.16 - Actions where lead Agency designation is necessary.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false Actions where lead Agency... DEVELOPMENT CORPORATION ENVIRONMENTAL QUALITY § 907.16 Actions where lead Agency designation is necessary. (a... proximity, the Corporation will seek designation as lead agency for those actions that directly relate...

  1. 36 CFR 1010.15 - Actions where lead agency designation is necessary.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... ENVIRONMENTAL QUALITY § 1010.15 Actions where lead agency designation is necessary. (a) Consistent with 40 CFR... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false Actions where lead agency... functional interdependence or geographical proximity, the Trust will seek designation as lead agency...

  2. 36 CFR 1010.15 - Actions where lead agency designation is necessary.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... ENVIRONMENTAL QUALITY § 1010.15 Actions where lead agency designation is necessary. (a) Consistent with 40 CFR... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false Actions where lead agency... functional interdependence or geographical proximity, the Trust will seek designation as lead agency...

  3. 36 CFR 1010.15 - Actions where lead agency designation is necessary.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... ENVIRONMENTAL QUALITY § 1010.15 Actions where lead agency designation is necessary. (a) Consistent with 40 CFR... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Actions where lead agency... functional interdependence or geographical proximity, the Trust will seek designation as lead agency...

  4. 36 CFR 1010.15 - Actions where lead agency designation is necessary.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... ENVIRONMENTAL QUALITY § 1010.15 Actions where lead agency designation is necessary. (a) Consistent with 40 CFR... 36 Parks, Forests, and Public Property 3 2013-07-01 2012-07-01 true Actions where lead agency... functional interdependence or geographical proximity, the Trust will seek designation as lead agency...

  5. 36 CFR 907.16 - Actions where lead Agency designation is necessary.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 3 2013-07-01 2012-07-01 true Actions where lead Agency... DEVELOPMENT CORPORATION ENVIRONMENTAL QUALITY § 907.16 Actions where lead Agency designation is necessary. (a... proximity, the Corporation will seek designation as lead agency for those actions that directly relate...

  6. 36 CFR 907.16 - Actions where lead Agency designation is necessary.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Actions where lead Agency... DEVELOPMENT CORPORATION ENVIRONMENTAL QUALITY § 907.16 Actions where lead Agency designation is necessary. (a... proximity, the Corporation will seek designation as lead agency for those actions that directly relate...

  7. 36 CFR 1010.15 - Actions where lead agency designation is necessary.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... ENVIRONMENTAL QUALITY § 1010.15 Actions where lead agency designation is necessary. (a) Consistent with 40 CFR... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Actions where lead agency... functional interdependence or geographical proximity, the Trust will seek designation as lead agency...

  8. 36 CFR 907.16 - Actions where lead Agency designation is necessary.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Actions where lead Agency... DEVELOPMENT CORPORATION ENVIRONMENTAL QUALITY § 907.16 Actions where lead Agency designation is necessary. (a... proximity, the Corporation will seek designation as lead agency for those actions that directly relate...

  9. 36 CFR 907.16 - Actions where lead Agency designation is necessary.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false Actions where lead Agency... DEVELOPMENT CORPORATION ENVIRONMENTAL QUALITY § 907.16 Actions where lead Agency designation is necessary. (a... proximity, the Corporation will seek designation as lead agency for those actions that directly relate...

  10. 45 CFR 1386.20 - Designated State Protection and Advocacy agency.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 4 2012-10-01 2012-10-01 false Designated State Protection and Advocacy agency... Advocacy of the Rights of Individuals with Developmental Disabilities § 1386.20 Designated State Protection and Advocacy agency. (a) The designating official must designate the State official or public...

  11. 77 FR 33227 - Assessment Questionnaire-IP Sector Specific Agency Risk Self Assessment Tool (IP-SSARSAT)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-05

    ... SECURITY Assessment Questionnaire--IP Sector Specific Agency Risk Self Assessment Tool (IP-SSARSAT) AGENCY... Protection and Programs Directorate (NPPD), Office of Infrastructure Protection (IP), Sector Outreach and...--Assessment Questionnaire--IP Sector Specific Agency Risk Self Assessment Tool (IP-SSARSAT). DHS...

  12. 76 FR 81955 - Assessment Questionnaire-IP Sector Specific Agency Risk Self Assessment Tool (IP-SSARSAT)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-29

    ... SECURITY Assessment Questionnaire--IP Sector Specific Agency Risk Self Assessment Tool (IP-SSARSAT) AGENCY... Protection and Programs Directorate (NPPD), Office of Infrastructure Protection (IP), Sector Specific Agency.../IP/SSA EMO, 245 Murray Lane SW., Mail Stop 0640, Arlington, VA 20598-0630. Emailed requests should...

  13. Space shuttle configuration accounting functional design specification

    NASA Technical Reports Server (NTRS)

    1974-01-01

    An analysis is presented of the requirements for an on-line automated system which must be capable of tracking the status of requirements and engineering changes and of providing accurate and timely records. The functional design specification provides the definition, description, and character length of the required data elements and the interrelationship of data elements to adequately track, display, and report the status of active configuration changes. As changes to the space shuttle program levels II and III configuration are proposed, evaluated, and dispositioned, it is the function of the configuration management office to maintain records regarding changes to the baseline and to track and report the status of those changes. The configuration accounting system will consist of a combination of computers, computer terminals, software, and procedures, all of which are designed to store, retrieve, display, and process information required to track proposed and proved engineering changes to maintain baseline documentation of the space shuttle program levels II and III.

  14. "Roll of Thunder, Hear My Cry": A Culturally Specific, Subversive Concept of Child Agency.

    ERIC Educational Resources Information Center

    McDowell, Kelly

    2002-01-01

    Presents a critique of Mildred D. Taylor's "Roll of Thunder, Hear My Cry" with regard to its positioning of the child subject. Proposes that the novel contrasts classic works of children's fiction by following a trajectory of child agency, which is enabled through the novel's racial specificity. Discusses the role of historical understanding and…

  15. 5 CFR 5201.102 - Designation of separate agency components.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... components. (a) Separate agency components of the Department of Labor. Pursuant to 5 CFR 2635.203(a), each of... purposes of applying any provision of 5 CFR part 2635 or this part to employees of the remainder of the...) Employment Standards Administration (ESA). (b) Separate agency subcomponents of ESA. Pursuant to 5 CFR...

  16. 5 CFR 5201.102 - Designation of separate agency components.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... components. (a) Separate agency components of the Department of Labor. Pursuant to 5 CFR 2635.203(a), each of... purposes of applying any provision of 5 CFR part 2635 or this part to employees of the remainder of the...) Employment Standards Administration (ESA). (b) Separate agency subcomponents of ESA. Pursuant to 5 CFR...

  17. 5 CFR 5201.102 - Designation of separate agency components.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... components. (a) Separate agency components of the Department of Labor. Pursuant to 5 CFR 2635.203(a), each of... purposes of applying any provision of 5 CFR part 2635 or this part to employees of the remainder of the...) Employment Standards Administration (ESA). (b) Separate agency subcomponents of ESA. Pursuant to 5 CFR...

  18. Design for a Performance Based Adult Education Community Coordinating Agency.

    ERIC Educational Resources Information Center

    Stambler, Moses

    A plan is described for a project to establish an adult education coordinating and change agency in the New Haven, Connecticut area to coordinate activities of existing organizations; provide a research staff to establish a computerized data base; disseminate information and provide technological services to local agencies; set up a communications…

  19. 5 CFR 5201.102 - Designation of separate agency components.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... components. (a) Separate agency components of the Department of Labor. Pursuant to 5 CFR 2635.203(a), each of... purposes of applying any provision of 5 CFR part 2635 or this part to employees of the remainder of the...) Employment Standards Administration (ESA). (b) Separate agency subcomponents of ESA. Pursuant to 5 CFR...

  20. Salt Bridges: Geometrically Specific, Designable Interactions

    PubMed Central

    Donald, Jason E.; Kulp, Daniel W.; DeGrado, William F.

    2010-01-01

    Salt bridges occur frequently in proteins, providing conformational specificity and contributing to molecular recognition and catalysis. We present a comprehensive analysis of these interactions in protein structures by surveying a large database of protein structures. Salt bridges between Asp or Glu and His, Arg, or Lys display extremely well-defined geometric preferences. Several previously observed preferences are confirmed and others that were previously unrecognized are discovered. Salt bridges are explored for their preferences for different separations in sequence and in space, geometric preferences within proteins and at protein-protein interfaces, cooperativity in networked salt bridges, inclusion within metal-binding sites, preference for acidic electrons, apparent conformational side chain entropy reduction upon formation, and degree of burial. Salt bridges occur far more frequently between residues at close than distant sequence separations, but at close distances there remain strong preferences for salt bridges at specific separations. Specific types of complex salt bridges, involving three or more members, are also discovered. As we observe a strong relationship between the propensity to form a salt bridge and the placement of salt-bridging residues in protein sequences, we discuss the role that salt bridges might play in kinetically influencing protein folding and thermodynamically stabilizing the native conformation. We also develop a quantitative method to select appropriate crystal structure resolution and B-factor cutoffs. Detailed knowledge of these geometric and sequence dependences should aid de novo design and prediction algorithms. PMID:21287621

  1. Salt bridges: geometrically specific, designable interactions.

    PubMed

    Donald, Jason E; Kulp, Daniel W; DeGrado, William F

    2011-03-01

    Salt bridges occur frequently in proteins, providing conformational specificity and contributing to molecular recognition and catalysis. We present a comprehensive analysis of these interactions in protein structures by surveying a large database of protein structures. Salt bridges between Asp or Glu and His, Arg, or Lys display extremely well-defined geometric preferences. Several previously observed preferences are confirmed, and others that were previously unrecognized are discovered. Salt bridges are explored for their preferences for different separations in sequence and in space, geometric preferences within proteins and at protein-protein interfaces, co-operativity in networked salt bridges, inclusion within metal-binding sites, preference for acidic electrons, apparent conformational side chain entropy reduction on formation, and degree of burial. Salt bridges occur far more frequently between residues at close than distant sequence separations, but, at close distances, there remain strong preferences for salt bridges at specific separations. Specific types of complex salt bridges, involving three or more members, are also discovered. As we observe a strong relationship between the propensity to form a salt bridge and the placement of salt-bridging residues in protein sequences, we discuss the role that salt bridges might play in kinetically influencing protein folding and thermodynamically stabilizing the native conformation. We also develop a quantitative method to select appropriate crystal structure resolution and B-factor cutoffs. Detailed knowledge of these geometric and sequence dependences should aid de novo design and prediction algorithms.

  2. Food Design Thinking: A Branch of Design Thinking Specific to Food Design

    ERIC Educational Resources Information Center

    Zampollo, Francesca; Peacock, Matthew

    2016-01-01

    Is there a need for a set of methods within Design Thinking tailored specifically for the Food Design process? Is there a need for a branch of Design Thinking dedicated to Food Design alone? Chefs are not generally trained in Design or Design Thinking, and we are only just beginning to understand how they ideate and what recourses are available to…

  3. 5 CFR 2638.203 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...) Information developed by internal audit and review staff, the Office of the Inspector General, if any, or... OFFICE OF GOVERNMENT ETHICS AND EXECUTIVE AGENCY ETHICS PROGRAM RESPONSIBILITIES Designated Agency Ethics... ethics official shall coordinate and manage the agency's ethics program. The program consists...

  4. 29 CFR 1601.80 - Certified designated FEP agencies.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... agencies receiving certification by the Commission are as follows: Alaska Commission for Human Rights Alexandria (VA) Human Rights Office Anchorage (AK) Equal Rights Commission Arizona Civil Rights Division Arlington County (VA) Human Rights Commission Austin Human Relations Commission Baltimore (MD)...

  5. 34 CFR 370.44 - What reporting requirement applies to each designated agency?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION CLIENT... designated agency from clients or client applicants; (d) The number of the requests for advocacy services... the designated agency was unable to serve all of the requests for advocacy services from clients...

  6. 34 CFR 370.44 - What reporting requirement applies to each designated agency?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION CLIENT... designated agency from clients or client applicants; (d) The number of the requests for advocacy services... the designated agency was unable to serve all of the requests for advocacy services from clients...

  7. Compiler writing system detail design specification. Volume 1: Language specification

    NASA Technical Reports Server (NTRS)

    Arthur, W. J.

    1974-01-01

    Construction within the Meta language for both language and target machine specification is reported. The elements of the function language as a meaning and syntax are presented, and the structure of the target language is described which represents the target dependent object text representation of applications programs.

  8. 5 CFR 5201.102 - Designation of separate agency components.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... components. (a) Separate agency components of the Department of Labor. Pursuant to 5 CFR 2635.203(a), each of... purposes of applying any provision of 5 CFR part 2635 or this part to employees of the remainder of the Department: (1) Benefits Review Board; (2) Employees Compensation Appeals Board; (3) Mine Safety and...

  9. 21 CFR 3.4 - Designated agency component.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... component charged with premarket review of devices shall have primary jurisdiction; (3) A biological product, the agency component charged with premarket review of biological products shall have primary... review and regulation of a combination product does not preclude consultations by that component...

  10. 15 CFR 923.47 - Designated State agency.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... (iv) Whether the agency is equipped to handle monitoring, evaluation and enforcement responsibilities. ... prudent use of administrative funds in the furtherance of the management and for monitoring of management... implementation vehicle. It is, however, the focal point for proper administration and evaluation of the...

  11. 29 CFR 1601.74 - Designated and notice agencies.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... to 29 CFR 1601.71(b). Austin (TX) Human Relations Commission 4 4 The Austin (TX) Human Relations... types of charges it shall be deemed a “Notice Agency,” pursuant to 29 CFR 1601.71(b). Baltimore (MD...” pursuant to 29 CFR 1601.71(b). Commonwealth of Puerto Rico Department of Labor 6 6The Commonwealth...

  12. 29 CFR 1601.74 - Designated and notice agencies.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... agencies are: Alaska Commission for Human Rights Alexandria (VA) Human Rights Office Allentown (PA) Human Relations Commission Anchorage (AK) Equal Rights Commission Anderson (IN) Human Relations Commission Arizona Civil Rights Division Arlington County (VA) Human Rights Commission 2 2 The Arlington Human......

  13. 46 CFR 162.050-21 - Separator: Design specification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 6 2011-10-01 2011-10-01 false Separator: Design specification. 162.050-21 Section 162... Separator: Design specification. (a) A separator must be designed to operate in each plane that forms an.... (c) Each separator component that is a moving part must be designed so that its movement...

  14. Bias and design in software specifications

    NASA Technical Reports Server (NTRS)

    Straub, Pablo A.; Zelkowitz, Marvin V.

    1990-01-01

    Implementation bias in a specification is an arbitrary constraint in the solution space. Presented here is a model of bias in software specifications. Bias is defined in terms of the specification process and a classification of the attributes of the software product. Our definition of bias provides insight into both the origin and the consequences of bias. It also shows that bias is relative and essentially unavoidable. Finally, we describe current work on defining a measure of bias, formalizing our model, and relating bias to software defects.

  15. 5 CFR 5501.102 - Designation of HHS components as separate agencies.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    .... Pursuant to 5 CFR 2635.203(a), each of the twelve components of HHS listed below is designated as an agency... agencies for purposes of applying any provision of 5 CFR part 2635 or this part to employees of the... Administration; (8) Health Resources and Services Administration; (9) Indian Health Service; (10)...

  16. As-built design specification for MISMAP

    NASA Technical Reports Server (NTRS)

    Brown, P. M.; Cheng, D. E.; Tompkins, M. A. (Principal Investigator)

    1981-01-01

    The MISMAP program, which is part of the CLASFYT package, is described. The program is designed to compare classification values with ground truth values for a segment and produce a comparison map and summary table.

  17. Radiological containment selection, design, and specification guide

    SciTech Connect

    Brown, R.L.

    1994-11-01

    This document provides guidance to Tank Waste Remediation Systems personnel in determining what containment is appropriate for work activities, what containments are available, general applications of each, design criteria, and other information needed to make informed decisions concerning containment application.

  18. 21 CFR 3.5 - Procedures for identifying the designated agency component.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... SERVICES GENERAL PRODUCT JURISDICTION Assignment of Agency Component for Review of Premarket Applications... and Research have entered into agreements clarifying product jurisdictional issues. These guidance... responsibility for categories of products or specific products. These intercenter agreements, and any...

  19. 7 CFR 281.5 - Responsibilities of an Indian tribal organization designated as State agency.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... ADMINISTRATION OF SNAP ON INDIAN RESERVATIONS § 281.5 Responsibilities of an Indian tribal organization designated as State agency. An ITO administering SNAP on a reservation shall adhere to the Food and...

  20. 40 CFR Appendix II to Part 280 - List of Agencies Designated To Receive Notifications

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 04333 Maryland (EPA Form), Science and Health Advisory Group, Office of Environmental Programs, 201 West... Notifications II Appendix II to Part 280 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... Designated To Receive Notifications Alabama (EPA Form), Alabama Department of Environmental...

  1. 40 CFR Appendix II to Part 280 - List of Agencies Designated To Receive Notifications

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 04333 Maryland (EPA Form), Science and Health Advisory Group, Office of Environmental Programs, 201 West... Notifications II Appendix II to Part 280 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... Designated To Receive Notifications Alabama (EPA Form), Alabama Department of Environmental...

  2. 40 CFR Appendix II to Part 280 - List of Agencies Designated To Receive Notifications

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 04333 Maryland (EPA Form), Science and Health Advisory Group, Office of Environmental Programs, 201 West... Notifications II Appendix II to Part 280 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... Designated To Receive Notifications Alabama (EPA Form), Alabama Department of Environmental...

  3. 40 CFR Appendix II to Part 280 - List of Agencies Designated To Receive Notifications

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 04333 Maryland (EPA Form), Science and Health Advisory Group, Office of Environmental Programs, 201 West... Notifications II Appendix II to Part 280 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... Designated To Receive Notifications Alabama (EPA Form), Alabama Department of Environmental...

  4. 40 CFR Appendix II to Part 280 - List of Agencies Designated To Receive Notifications

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 04333 Maryland (EPA Form), Science and Health Advisory Group, Office of Environmental Programs, 201 West... Notifications II Appendix II to Part 280 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... Designated To Receive Notifications Alabama (EPA Form), Alabama Department of Environmental...

  5. IMCS reflight certification requirements and design specifications

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The requirements for reflight certification are established. Software requirements encompass the software programs that are resident in the PCC, DEP, PDSS, EC, or any related GSE. A design approach for the reflight software packages is recommended. These designs will be of sufficient detail to permit the implementation of reflight software. The PDSS/IMC Reflight Certification system provides the tools and mechanisms for the user to perform the reflight certification test procedures, test data capture, test data display, and test data analysis. The system as defined will be structured to permit maximum automation of reflight certification procedures and test data analysis.

  6. 40 CFR 152.95 - Citation of all studies in the Agency's files pertinent to a specific data requirement.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 25 2012-07-01 2012-07-01 false Citation of all studies in the Agency's files pertinent to a specific data requirement. 152.95 Section 152.95 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PESTICIDE REGISTRATION AND...

  7. 49 CFR 107.402 - Application for designation as a certification agency.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ....402 Application for designation as a certification agency. (a) Any organization or person seeking to... organization, by an authorized officer or official representative of the organization. Any false statement or... proof that the applicant has: (i) The ability to review and evaluate design drawings, design and...

  8. 41 CFR 102-76.20 - What issues must Federal agencies consider in providing site planning and landscape design services?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... agencies consider in providing site planning and landscape design services? 102-76.20 Section 102-76.20... What issues must Federal agencies consider in providing site planning and landscape design services? In providing site planning and design services, Federal agencies must— (a) Make the site planning and...

  9. 41 CFR 102-76.20 - What issues must Federal agencies consider in providing site planning and landscape design services?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... agencies consider in providing site planning and landscape design services? 102-76.20 Section 102-76.20... What issues must Federal agencies consider in providing site planning and landscape design services? In providing site planning and design services, Federal agencies must— (a) Make the site planning and...

  10. 15 CFR 923.47 - Designated State agency.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...) NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE OCEAN AND COASTAL RESOURCE MANAGEMENT COASTAL ZONE MANAGEMENT PROGRAM REGULATIONS Authorities and Organization § 923.47 Designated State... make periodic reports to the Office of Ocean and Coastal Resource Management (OCRM), the Governor,...

  11. 15 CFR 923.47 - Designated State agency.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...) NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE OCEAN AND COASTAL RESOURCE MANAGEMENT COASTAL ZONE MANAGEMENT PROGRAM REGULATIONS Authorities and Organization § 923.47 Designated State... make periodic reports to the Office of Ocean and Coastal Resource Management (OCRM), the Governor,...

  12. 15 CFR 923.47 - Designated State agency.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...) NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE OCEAN AND COASTAL RESOURCE MANAGEMENT COASTAL ZONE MANAGEMENT PROGRAM REGULATIONS Authorities and Organization § 923.47 Designated State... make periodic reports to the Office of Ocean and Coastal Resource Management (OCRM), the Governor,...

  13. 15 CFR 923.47 - Designated State agency.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...) NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE OCEAN AND COASTAL RESOURCE MANAGEMENT COASTAL ZONE MANAGEMENT PROGRAM REGULATIONS Authorities and Organization § 923.47 Designated State... make periodic reports to the Office of Ocean and Coastal Resource Management (OCRM), the Governor,...

  14. 46 CFR 162.050-33 - Bilge alarm: Design specification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 6 2011-10-01 2011-10-01 false Bilge alarm: Design specification. 162.050-33 Section....050-33 Bilge alarm: Design specification. (a) This section contains requirements that apply to bilge alarms. (b) Each bilge alarm must be designed to meet the requirements for an oil content meter in §...

  15. AFEII Analog Front End Board Design Specifications

    SciTech Connect

    Rubinov, Paul; /Fermilab

    2005-04-01

    This document describes the design of the 2nd iteration of the Analog Front End Board (AFEII), which has the function of receiving charge signals from the Central Fiber Tracker (CFT) and providing digital hit pattern and charge amplitude information from those charge signals. This second iteration is intended to address limitations of the current AFE (referred to as AFEI in this document). These limitations become increasingly deleterious to the performance of the Central Fiber Tracker as instantaneous luminosity increases. The limitations are inherent in the design of the key front end chips on the AFEI board (the SVXIIe and the SIFT) and the architecture of the board itself. The key limitations of the AFEI are: (1) SVX saturation; (2) Discriminator to analog readout cross talk; (3) Tick to tick pedestal variation; and (4) Channel to channel pedestal variation. The new version of the AFE board, AFEII, addresses these limitations by use of a new chip, the TriP-t and by architectural changes, while retaining the well understood and desirable features of the AFEI board.

  16. 46 CFR 162.050-21 - Separator: Design specification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Separator: Design specification. (a) A separator must be designed to operate in each plane that forms an angle of 22.5° with the plane of its normal operating position. (b) The electrical components of...

  17. 46 CFR 162.050-21 - Separator: Design specification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Separator: Design specification. (a) A separator must be designed to operate in each plane that forms an angle of 22.5° with the plane of its normal operating position. (b) The electrical components of...

  18. 46 CFR 162.050-21 - Separator: Design specification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... MATERIALS: SPECIFICATIONS AND APPROVAL ENGINEERING EQUIPMENT Pollution Prevention Equipment § 162.050-21 Separator: Design specification. (a) A separator must be designed to operate in each plane that forms an angle of 22.5° with the plane of its normal operating position. (b) The electrical components of...

  19. Gateway design specification for fiber optic local area networks

    NASA Technical Reports Server (NTRS)

    1985-01-01

    This is a Design Specification for a gateway to interconnect fiber optic local area networks (LAN's). The internetworking protocols for a gateway device that will interconnect multiple local area networks are defined. This specification serves as input for preparation of detailed design specifications for the hardware and software of a gateway device. General characteristics to be incorporated in the gateway such as node address mapping, packet fragmentation, and gateway routing features are described.

  20. 41 CFR 102-76.20 - What issues must Federal agencies consider in providing site planning and landscape design services?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... agencies consider in providing site planning and landscape design services? 102-76.20 Section 102-76.20...) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design and Construction § 102-76.20 What issues must Federal agencies consider in providing site planning and landscape design services?...

  1. 41 CFR 102-76.20 - What issues must Federal agencies consider in providing site planning and landscape design services?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... agencies consider in providing site planning and landscape design services? 102-76.20 Section 102-76.20...) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design and Construction § 102-76.20 What issues must Federal agencies consider in providing site planning and landscape design services?...

  2. 41 CFR 102-76.20 - What issues must Federal agencies consider in providing site planning and landscape design services?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... agencies consider in providing site planning and landscape design services? 102-76.20 Section 102-76.20...) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design and Construction § 102-76.20 What issues must Federal agencies consider in providing site planning and landscape design services?...

  3. 41 CFR 102-76.25 - What standards must Federal agencies meet in providing architectural and interior design services?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Federal agencies meet in providing architectural and interior design services? 102-76.25 Section 102-76.25...) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design and Construction § 102-76.25 What standards must Federal agencies meet in providing architectural and interior design...

  4. Functional design specification for the problem data system. [space shuttle

    NASA Technical Reports Server (NTRS)

    Boatman, T. W.

    1975-01-01

    The purpose of the Functional Design Specification is to outline the design for the Problem Data System. The Problem Data System is a computer-based data management system designed to track the status of problems and corrective actions pertinent to space shuttle hardware.

  5. UXO Engineering Design. Technical Specification and ConceptualDesign

    SciTech Connect

    Beche, J-F.; Doolittle, L.; Greer, J.; Lafever, R.; Radding, Z.; Ratti, A.; Yaver, H.; Zimmermann, S.

    2005-04-23

    The design and fabrication of the UXO detector has numerous challenges and is an important component to the success of this study. This section describes the overall engineering approach, as well as some of the technical details that brought us to the present design. In general, an array of sensor coils is measuring the signal generated by the UXO object in response to a stimulation provided by the driver coil. The information related to the location, shape and properties of the object is derived from the analysis of the measured data. Each sensor coil is instrumented with a waveform digitizer operating at a nominal digitization rate of 100 kSamples per second. The sensor coils record both the large transient pulse of the driver coil and the UXO object response pulse. The latter is smaller in amplitude and must be extracted from the large transient signal. The resolution required is 16 bits over a dynamic range of at least 140 dB. The useful signal bandwidth of the application extends from DC to 40 kHz. The low distortion of each component is crucial in order to maintain an excellent linearity over the full dynamic range and to minimize the calibration procedure. The electronics must be made as compact as possible so that the response of its metallic parts has a minimum signature response. Also because of a field system portability requirement, the power consumption of the instrument must be kept as low as possible. The theory and results of numerical and experimental studies that led to the proof-of-principle multitransmitter-multireceiver Active ElectroMagnetic (AEM) system, that can not only accurately detect but also characterize and discriminate UXO targets, are summarized in LBNL report-53962: ''Detection and Classification of Buried Metallic Objects, UX-1225''.

  6. Working Notes from the 1992 AAAI Workshop on Automating Software Design. Theme: Domain Specific Software Design

    NASA Technical Reports Server (NTRS)

    Keller, Richard M. (Editor); Barstow, David; Lowry, Michael R.; Tong, Christopher H.

    1992-01-01

    The goal of this workshop is to identify different architectural approaches to building domain-specific software design systems and to explore issues unique to domain-specific (vs. general-purpose) software design. Some general issues that cut across the particular software design domain include: (1) knowledge representation, acquisition, and maintenance; (2) specialized software design techniques; and (3) user interaction and user interface.

  7. SEPAC flight software detailed design specifications, volume 1

    NASA Technical Reports Server (NTRS)

    1982-01-01

    The detailed design specifications (as built) for the SEPAC Flight Software are defined. The design includes a description of the total software system and of each individual module within the system. The design specifications describe the decomposition of the software system into its major components. The system structure is expressed in the following forms: the control-flow hierarchy of the system, the data-flow structure of the system, the task hierarchy, the memory structure, and the software to hardware configuration mapping. The component design description includes details on the following elements: register conventions, module (subroutines) invocaton, module functions, interrupt servicing, data definitions, and database structure.

  8. 34 CFR 370.7 - What shall the designated agency do to make its services accessible?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION CLIENT... accessible to clients or client applicants who seek or receive CAP services. (Authority: 29 U.S.C. 711(c)) ... 34 Education 2 2011-07-01 2010-07-01 true What shall the designated agency do to make its...

  9. 34 CFR 370.14 - How does a designated agency appeal a written decision to redesignate?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Education (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION CLIENT ASSISTANCE PROGRAM What Requirements Apply to Redesignation? § 370.14 How does a designated agency... with the Secretary will be determined in a manner consistent with the requirements of 34 CFR 81.12....

  10. 34 CFR 370.7 - What shall the designated agency do to make its services accessible?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... CLIENT ASSISTANCE PROGRAM General § 370.7 What shall the designated agency do to make its services... formats that are accessible to clients or client applicants who seek or receive CAP services. (Authority... services accessible? 370.7 Section 370.7 Education Regulations of the Offices of the Department...

  11. 34 CFR 370.14 - How does a designated agency appeal a written decision to redesignate?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Education (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION CLIENT ASSISTANCE PROGRAM What Requirements Apply to Redesignation? § 370.14 How does a designated agency... with the Secretary will be determined in a manner consistent with the requirements of 34 CFR 81.12....

  12. 7 CFR 281.5 - Responsibilities of an Indian tribal organization designated as State agency.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM ADMINISTRATION OF THE FOOD STAMP PROGRAM ON INDIAN RESERVATIONS § 281.5 Responsibilities of an Indian tribal organization designated as State agency. An ITO administering the Food Stamp Program on a reservation...

  13. A Conceptual Approach to the Design of Management Information Systems for State Vocational Rehabilitation Agencies.

    ERIC Educational Resources Information Center

    Portny, Stanley E.

    State vocational rehabilitation agencies need a system for effectively and efficiently collecting, processing, and reporting program information. Design of such a system begins with a thorough understanding of the nature of the information needs and the particular applications for which the information will be used. Information needs may be…

  14. 76 FR 45297 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; Design of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-28

    ... toll-free number) or by e-mail at DOL_PRA_PUBLIC@dol.gov . SUPPLEMENTARY INFORMATION: The design of... information, see the related notice published in the Federal Register on April 6, 2011 (76 FR 19129... of the Secretary Agency Information Collection Activities; Submission for OMB Review; Comment...

  15. 77 FR 55488 - Agency Information Collection Activities: Designation of Attorney in Fact

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-10

    ... SECURITY United States Immigration and Customs Enforcement Agency Information Collection Activities: Designation of Attorney in Fact The Department of Homeland Security, U.S. Immigration and Customs Enforcement... Budget. Comments should be addressed to OMB Desk Officer, for United States Immigration and...

  16. 49 CFR 107.402 - Application for designation as an approval or certification agency.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... organization or person seeking designation as an approval or certification agency shall apply in writing to the... and certified to be correct by the applicant or, if the applicant is an organization, by an authorized officer or official representative of the organization. Any false statement or representation, or...

  17. 49 CFR 107.402 - Application for designation as an approval or certification agency.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... organization or person seeking designation as an approval or certification agency shall apply in writing to the... and certified to be correct by the applicant or, if the applicant is an organization, by an authorized officer or official representative of the organization. Any false statement or representation, or...

  18. 34 CFR 367.10 - How does a designated State agency (DSA) apply for an award?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true How does a designated State agency (DSA) apply for an award? 367.10 Section 367.10 Education Regulations of the Offices of the Department of Education... LIVING SERVICES FOR OLDER INDIVIDUALS WHO ARE BLIND What Are the Application Requirements? § 367.10...

  19. Epitope-Specific Binder Design by Yeast Surface Display.

    PubMed

    Mann, Jasdeep K; Park, Sheldon

    2015-01-01

    Yeast surface display is commonly used to engineer affinity and design novel molecular interaction. By alternating positive and negative selections, yeast display can be used to engineer binders that specifically interact with the target protein at a defined site. Epitope-specific binders can be useful as inhibitors if they bind the target molecule at functionally important sites. Therefore, an efficient method of engineering epitope specificity should help with the engineering of inhibitors. We describe the use of yeast surface display to design single domain monobodies that bind and inhibit the activity of the kinase Erk-2 by targeting a conserved surface patch involved in protein-protein interaction. The designed binders can be used to disrupt signaling in the cell and investigate Erk-2 function in vivo. The described protocol is general and can be used to design epitope-specific binders of an arbitrary protein. PMID:26060073

  20. A Learning Design Ontology Based on the IMS Specification

    ERIC Educational Resources Information Center

    Amorim, Ricardo R.; Lama, Manuel; Sanchez, Eduardo; Riera, Adolfo; Vila, Xose A.

    2006-01-01

    In this paper, we present an ontology to represent the semantics of the IMS Learning Design (IMS LD) specification, a meta-language used to describe the main elements of the learning design process. The motivation of this work relies on the expressiveness limitations found on the current XML-Schema implementation of the IMS LD conceptual model. To…

  1. Electrostatic precipitator guidelines: Volume 1, Design specifications: Final report

    SciTech Connect

    Altin, C.A.; Grieco, G.J.

    1987-06-01

    The report includes three companion manuals for design specifications, operations and maintenance, and troubleshooting. Although the manuals primarily address users having some knowledge of precipitator design and operation, they provide enough background material and precipitator theory to make them useful as training aids. The loose-leaf format will allow updating.

  2. 40 CFR 152.95 - Citation of all studies in the Agency's files pertinent to a specific data requirement.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 25 2013-07-01 2013-07-01 false Citation of all studies in the Agency's files pertinent to a specific data requirement. 152.95 Section 152.95 Protection of Environment... PROCEDURES Procedures To Ensure Protection of Data Submitters' Rights § 152.95 Citation of all studies in...

  3. 40 CFR 152.95 - Citation of all studies in the Agency's files pertinent to a specific data requirement.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 24 2014-07-01 2014-07-01 false Citation of all studies in the Agency's files pertinent to a specific data requirement. 152.95 Section 152.95 Protection of Environment... PROCEDURES Satisfaction of Data Requirements and Protection of Data Submitters' Rights § 152.95 Citation...

  4. Homolog-specific PCR primer design for profiling splice variants

    PubMed Central

    Srivastava, Gyan Prakash; Hanumappa, Mamatha; Kushwaha, Garima; Nguyen, Henry T.; Xu, Dong

    2011-01-01

    To study functional diversity of proteins encoded from a single gene, it is important to distinguish the expression levels among the alternatively spliced variants. A variant-specific primer pair is required to amplify each alternatively spliced variant individually. For this purpose, we developed a new feature, homolog-specific primer design (HSPD), in our high-throughput primer and probe design software tool, PRIMEGENS-v2. The algorithm uses a de novo approach to design primers without any prior information of splice variants or close homologs for an input query sequence. It not only designs primer pairs but also finds potential isoforms and homologs of the input sequence. Efficiency of this algorithm was tested for several gene families in soybean. A total of 187 primer pairs were tested under five different abiotic stress conditions with three replications at three time points. Results indicate a high success rate of primer design. Some primer pairs designed were able to amplify all splice variants of a gene. Furthermore, by utilizing combinations within the same multiplex pool, we were able to uniquely amplify a specific variant or duplicate gene. Our method can also be used to design PCR primers to specifically amplify homologs in the same gene family. PRIMEGENS-v2 is available at: http://primegens.org. PMID:21415011

  5. Designing Domain-Specific HUMS Architectures: An Automated Approach

    NASA Technical Reports Server (NTRS)

    Mukkamala, Ravi; Agarwal, Neha; Kumar, Pramod; Sundaram, Parthiban

    2004-01-01

    The HUMS automation system automates the design of HUMS architectures. The automated design process involves selection of solutions from a large space of designs as well as pure synthesis of designs. Hence the whole objective is to efficiently search for or synthesize designs or parts of designs in the database and to integrate them to form the entire system design. The automation system adopts two approaches in order to produce the designs: (a) Bottom-up approach and (b) Top down approach. Both the approaches are endowed with a Suite of quantitative and quantitative techniques that enable a) the selection of matching component instances, b) the determination of design parameters, c) the evaluation of candidate designs at component-level and at system-level, d) the performance of cost-benefit analyses, e) the performance of trade-off analyses, etc. In short, the automation system attempts to capitalize on the knowledge developed from years of experience in engineering, system design and operation of the HUMS systems in order to economically produce the most optimal and domain-specific designs.

  6. 3 CFR - Designation of Officials of the Court Services and Offender Supervision Agency to Act as Director

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 3 The President 1 2011-01-01 2011-01-01 false Designation of Officials of the Court Services and Offender Supervision Agency to Act as Director Presidential Documents Other Presidential Documents Memorandum of August 17, 2010 Designation of Officials of the Court Services and Offender Supervision Agency to Act as Director Memorandum for...

  7. 28 CFR 81.2 - Submission of reports; designation of agencies to receive reports of child abuse.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... agencies to receive reports of child abuse. 81.2 Section 81.2 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.2 Submission of reports; designation of agencies to receive reports of child abuse. Reports of child abuse required by...

  8. 28 CFR 81.2 - Submission of reports; designation of agencies to receive reports of child abuse.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... agencies to receive reports of child abuse. 81.2 Section 81.2 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.2 Submission of reports; designation of agencies to receive reports of child abuse. Reports of child abuse required by...

  9. 28 CFR 81.2 - Submission of reports; designation of agencies to receive reports of child abuse.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... agencies to receive reports of child abuse. 81.2 Section 81.2 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.2 Submission of reports; designation of agencies to receive reports of child abuse. Reports of child abuse required by...

  10. 28 CFR 81.2 - Submission of reports; designation of agencies to receive reports of child abuse.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... agencies to receive reports of child abuse. 81.2 Section 81.2 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.2 Submission of reports; designation of agencies to receive reports of child abuse. Reports of child abuse required by...

  11. 28 CFR 81.2 - Submission of reports; designation of agencies to receive reports of child abuse.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... agencies to receive reports of child abuse. 81.2 Section 81.2 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.2 Submission of reports; designation of agencies to receive reports of child abuse. Reports of child abuse required by...

  12. 41 CFR 102-193.20 - What are the specific agency responsibilities for records management?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Property Management Federal Property Management Regulations System (Continued) FEDERAL MANAGEMENT... and NARA regulations in 36 CFR chapter XII to carry out your records management responsibilities. To... agencywide records management programs to an office of the agency and to a qualified records manager....

  13. Design specifications for manufacturability of MCM-C multichip modules

    SciTech Connect

    Allen, C.; Blazek, R.; Desch, J.; Elarton, J.; Kautz, D.; Markley, D.; Morgenstern, H.; Stewart, R.; Warner, L.

    1995-06-01

    The scope of this document is to establish design guidelines for electronic circuitry packaged as multichip modules of the ceramic substrate variety, although many of these guidelines are applicable to other types of multichip modules. The guidelines begin with prerequisite information which must be developed between customer and designer of the multichip module. The core of the guidelines focuses on the many considerations that must be addressed during the multichip module design. The guidelines conclude with the resulting deliverables from the design which satisfy customer requirements and/or support the multichip module fabrication and testing processes. Considerable supporting information, checklists, and design constraints are captured in specific appendices and used as reference information in the main body text. Finally some real examples of multichip module design are presented.

  14. 41 CFR 102-83.120 - What happens if an agency has a need to be in a specific urban area that is not a central city in...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... agency has a need to be in a specific urban area that is not a central city in a metropolitan area? 102... Space Urban Areas § 102-83.120 What happens if an agency has a need to be in a specific urban area that is not a central city in a metropolitan area? If an agency has a need to be in a specific urban...

  15. 41 CFR 102-83.120 - What happens if an agency has a need to be in a specific urban area that is not a central city in...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... agency has a need to be in a specific urban area that is not a central city in a metropolitan area? 102... Space Urban Areas § 102-83.120 What happens if an agency has a need to be in a specific urban area that is not a central city in a metropolitan area? If an agency has a need to be in a specific urban...

  16. Specification, Design, and Analysis of Advanced HUMS Architectures

    NASA Technical Reports Server (NTRS)

    Mukkamala, Ravi

    2004-01-01

    During the two-year project period, we have worked on several aspects of domain-specific architectures for HUMS. In particular, we looked at using scenario-based approach for the design and designed a language for describing such architectures. The language is now being used in all aspects of our HUMS design. In particular, we have made contributions in the following areas. 1) We have employed scenarios in the development of HUMS in three main areas. They are: (a) To improve reusability by using scenarios as a library indexing tool and as a domain analysis tool; (b) To improve maintainability by recording design rationales from two perspectives - problem domain and solution domain; (c) To evaluate the software architecture. 2) We have defined a new architectural language called HADL or HUMS Architectural Definition Language. It is a customized version of xArch/xADL. It is based on XML and, hence, is easily portable from domain to domain, application to application, and machine to machine. Specifications written in HADL can be easily read and parsed using the currently available XML parsers. Thus, there is no need to develop a plethora of software to support HADL. 3) We have developed an automated design process that involves two main techniques: (a) Selection of solutions from a large space of designs; (b) Synthesis of designs. However, the automation process is not an absolute Artificial Intelligence (AI) approach though it uses a knowledge-based system that epitomizes a specific HUMS domain. The process uses a database of solutions as an aid to solve the problems rather than creating a new design in the literal sense. Since searching is adopted as the main technique, the challenges involved are: (a) To minimize the effort in searching the database where a very large number of possibilities exist; (b) To develop representations that could conveniently allow us to depict design knowledge evolved over many years; (c) To capture the required information that aid the

  17. Integration of site-specific health information: Agency for Toxic Substances and Disease Registry health assessments

    SciTech Connect

    Lesperance, A.M.; Siegel, M.R.

    1990-12-01

    The Agency for Toxic Substances and Disease Registry is required to conduct a health assessment of any site that is listed on or proposed for the US Environmental Protection Agency's National Priorities List. Sixteen US Department of Energy (DOE) sites currently fall into this category. Health assessments contain a qualitative description of impacts to public health and the environment from hazardous waste sites, as well as recommendations for actions to mitigate or eliminate risk. Because these recommendations may have major impacts on compliance activities at DOE facilities, the health assessments are an important source of information for the monitoring activities of DOE's Office of Environmental Compliance (OEC). This report provides an overview of the activities involved in preparing the health assessment, its role in environmental management, and its key elements.

  18. NASA software specification and evaluation system design, part 2

    NASA Technical Reports Server (NTRS)

    1976-01-01

    A survey and analysis of the existing methods, tools and techniques employed in the development of software are presented along with recommendations for the construction of reliable software. Functional designs for software specification language, and the data base verifier are presented.

  19. A design guide and specification for small explosive containment structures

    SciTech Connect

    Marchand, K.A.; Cox, P.A.; Polcyn, M.A.

    1994-12-01

    The design of structural containments for testing small explosive devices requires the designer to consider the various aspects of the explosive loading, i.e., shock and gas or quasistatic pressure. Additionally, if the explosive charge has the potential of producing damaging fragments, provisions must be made to arrest the fragments. This may require that the explosive be packed in a fragment attenuating material, which also will affect the loads predicted for containment response. Material also may be added just to attenuate shock, in the absence of fragments. Three charge weights are used in the design. The actual charge is used to determine a design fragment. Blast loads are determined for a {open_quotes}design charge{close_quotes}, defined as 125% of the operational charge in the explosive device. No yielding is permitted at the design charge weight. Blast loads are also determined for an over-charge, defined as 200% of the operational charge in the explosive device. Yielding, but no failure, is permitted at this over-charge. This guide emphasizes the calculation of loads and fragments for which the containment must be designed. The designer has the option of using simplified or complex design-analysis methods. Examples in the guide use readily available single degree-of-freedom (sdof) methods, plus static methods for equivalent dynamic loads. These are the common methods for blast resistant design. Some discussion of more complex methods is included. Generally, the designer who chooses more complex methods must be fully knowledgeable in their use and limitations. Finally, newly fabricated containments initially must be proof tested to 125% of the operational load and then inspected at regular intervals. This specification provides guidance for design, proof testing, and inspection of small explosive containment structures.

  20. Highly specific protein-protein interactions, evolution and negative design.

    PubMed

    Sear, Richard P

    2004-12-01

    We consider highly specific protein-protein interactions in proteomes of simple model proteins. We are inspired by the work of Zarrinpar et al (2003 Nature 426 676). They took a binding domain in a signalling pathway in yeast and replaced it with domains of the same class but from different organisms. They found that the probability of a protein binding to a protein from the proteome of a different organism is rather high, around one half. We calculate the probability of a model protein from one proteome binding to the protein of a different proteome. These proteomes are obtained by sampling the space of functional proteomes uniformly. In agreement with Zarrinpar et al we find that the probability of a protein binding a protein from another proteome is rather high, of order one tenth. Our results, together with those of Zarrinpar et al, suggest that designing, say, a peptide to block or reconstitute a single signalling pathway, without affecting any other pathways, requires knowledge of all the partners of the class of binding domains the peptide is designed to mimic. This knowledge is required to use negative design to explicitly design out interactions of the peptide with proteins other than its target. We also found that patches that are required to bind with high specificity evolve more slowly than those that are required only to not bind to any other patch. This is consistent with some analysis of sequence data for proteins engaged in highly specific interactions.

  1. High specific energy, high capacity nickel-hydrogen cell design

    NASA Technical Reports Server (NTRS)

    Wheeler, James R.

    1993-01-01

    A 3.5 inch rabbit-ear-terminal nickel-hydrogen cell has been designed and tested to deliver high capacity at a C/1.5 discharge rate. Its specific energy yield of 60.6 wh/kg is believed to be the highest yet achieved in a slurry-process nickel-hydrogen cell, and its 10 C capacity of 113.9 AH the highest capacity yet made at a discharge rate this high in the 3.5 inch diameter size. The cell also demonstrated a pulse capability of 180 amps for 20 seconds. Specific cell parameters, performance, and future test plans are described.

  2. Space tug thermal control. [design criteria and specifications

    NASA Technical Reports Server (NTRS)

    1974-01-01

    It was determined that space tug will require the capability to perform its mission within a broad range of thermal environments with currently planned mission durations of up to seven days, so an investigation was conducted to define a thermal design for the forward and intertank compartments and fuel cell heat rejection system that satisfies tug requirements for low inclination geosynchronous deploy and retrieve missions. Passive concepts were demonstrated analytically for both the forward and intertank compartments, and a worst case external heating environment was determined for use during the study. The thermal control system specifications and designs which resulted from the research are shown.

  3. Specific issues of the design for the elderly

    NASA Astrophysics Data System (ADS)

    Sebesi, S. B.; Groza, H. L.; Ianoşi, A.; Dimitrova, A.; Mândru, D.

    2016-08-01

    The actual demographic studies show that number of the elderly people is increasing constantly. Considering their motor, sensorial and cognitive constrains and restrictions, a new field of Assistive Technology is developing, focussed on the design and development of a wide range of devices, apparatus, equipment and systems dedicated to their independent and safe life. In this paper, a systematisation of existing gero-technical systems is proposed, emphasising the today trends in this field. The specific issues of designing this kind of products are identified and analysed. Two approaches of the authors are finally presented: wearable suits for aging and disabilities simulation and tele-monitoring of the elderly.

  4. 40 CFR 33.406 - May a recipient designate a lead agency for fair share objective negotiation purposes?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE PARTICIPATION BY DISADVANTAGED BUSINESS ENTERPRISES IN UNITED STATES ENVIRONMENTAL PROTECTION AGENCY PROGRAMS Fair Share Objectives § 33.406 May a... 40 Protection of Environment 1 2010-07-01 2010-07-01 false May a recipient designate a lead...

  5. 40 CFR 33.406 - May a recipient designate a lead agency for fair share objective negotiation purposes?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE PARTICIPATION BY DISADVANTAGED BUSINESS ENTERPRISES IN UNITED STATES ENVIRONMENTAL PROTECTION AGENCY PROGRAMS Fair Share Objectives § 33.406 May a... 40 Protection of Environment 1 2011-07-01 2011-07-01 false May a recipient designate a lead...

  6. 40 CFR 33.406 - May a recipient designate a lead agency for fair share objective negotiation purposes?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE PARTICIPATION BY DISADVANTAGED BUSINESS ENTERPRISES IN UNITED STATES ENVIRONMENTAL PROTECTION AGENCY PROGRAMS Fair Share Objectives § 33.406 May a... 40 Protection of Environment 1 2014-07-01 2014-07-01 false May a recipient designate a lead...

  7. 40 CFR 33.406 - May a recipient designate a lead agency for fair share objective negotiation purposes?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE PARTICIPATION BY DISADVANTAGED BUSINESS ENTERPRISES IN UNITED STATES ENVIRONMENTAL PROTECTION AGENCY PROGRAMS Fair Share Objectives § 33.406 May a... 40 Protection of Environment 1 2012-07-01 2012-07-01 false May a recipient designate a lead...

  8. 29 CFR 1640.9 - Processing of complaints or charges of employment discrimination filed with a designated agency...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 4 2010-07-01 2010-07-01 false Processing of complaints or charges of employment discrimination filed with a designated agency and either a section 504 agency, the EEOC, or both. 1640.9 Section 1640.9 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY...

  9. 29 CFR 1640.9 - Processing of complaints or charges of employment discrimination filed with a designated agency...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 4 2011-07-01 2011-07-01 false Processing of complaints or charges of employment discrimination filed with a designated agency and either a section 504 agency, the EEOC, or both. 1640.9 Section 1640.9 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY...

  10. 41 CFR 102-76.30 - What seismic safety standards must Federal agencies follow in the design and construction of...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 41 Public Contracts and Property Management 3 2013-07-01 2013-07-01 false What seismic safety... § 102-76.30 What seismic safety standards must Federal agencies follow in the design and construction of Federal facilities? Federal agencies must follow the seismic safety standards identified in § 102-80.45...

  11. 41 CFR 102-76.30 - What seismic safety standards must Federal agencies follow in the design and construction of...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 41 Public Contracts and Property Management 3 2011-01-01 2011-01-01 false What seismic safety... § 102-76.30 What seismic safety standards must Federal agencies follow in the design and construction of Federal facilities? Federal agencies must follow the seismic safety standards identified in § 102-80.45...

  12. 41 CFR 102-76.30 - What seismic safety standards must Federal agencies follow in the design and construction of...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 41 Public Contracts and Property Management 3 2012-01-01 2012-01-01 false What seismic safety... § 102-76.30 What seismic safety standards must Federal agencies follow in the design and construction of Federal facilities? Federal agencies must follow the seismic safety standards identified in § 102-80.45...

  13. 41 CFR 102-76.30 - What seismic safety standards must Federal agencies follow in the design and construction of...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 41 Public Contracts and Property Management 3 2014-01-01 2014-01-01 false What seismic safety... § 102-76.30 What seismic safety standards must Federal agencies follow in the design and construction of Federal facilities? Federal agencies must follow the seismic safety standards identified in § 102-80.45...

  14. 41 CFR 102-76.30 - What seismic safety standards must Federal agencies follow in the design and construction of...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false What seismic safety... § 102-76.30 What seismic safety standards must Federal agencies follow in the design and construction of Federal facilities? Federal agencies must follow the seismic safety standards identified in § 102-80.45...

  15. Precise Specification of Design Pattern Structure and Behaviour

    NASA Astrophysics Data System (ADS)

    Sterritt, Ashley; Clarke, Siobhán; Cahill, Vinny

    Applying design patterns while developing a software system can improve its non-functional properties, such as extensibility and loose coupling. Precise specification of structure and behaviour communicates the invariants imposed by a pattern on a conforming implementation and enables formal software verification. Many existing design-pattern specification languages (DPSLs) focus on class structure alone, while those that do address behaviour suffer from a lack of expressiveness and/or imprecise semantics. In particular, in a review of existing work, three invariant categories were found to be inexpressible in state-of-the-art DPSLs: dependency, object state and data-structure. This paper presents Alas: a precise specification language that supports design-pattern descriptions including these invariant categories. The language is based on UML Class and Sequence diagrams with modified syntax and semantics. In this paper, the meaning of the presented invariants is formalized and relevant ambiguities in the UML Standard are clarified. We have evaluated Alas by specifying the widely-used Gang of Four pattern catalog and identified patterns that benefitted from the added expressiveness and semantics of Alas.

  16. 41 CFR 102-74.235 - Are occupant agencies required to cooperate with the Designated Official in the implementation of...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... required to cooperate with the Designated Official in the implementation of the emergency plans and the... agencies required to cooperate with the Designated Official in the implementation of the emergency plans... cooperate with the Designated Official in the implementation of the emergency plans and the staffing of...

  17. 41 CFR 102-74.235 - Are occupant agencies required to cooperate with the Designated Official in the implementation of...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... required to cooperate with the Designated Official in the implementation of the emergency plans and the... agencies required to cooperate with the Designated Official in the implementation of the emergency plans... cooperate with the Designated Official in the implementation of the emergency plans and the staffing of...

  18. Flexible Mx specification of various extended twin kinship designs.

    PubMed

    Maes, Hermine H; Neale, Michael C; Medland, Sarah E; Keller, Matthew C; Martin, Nicholas G; Heath, Andrew C; Eaves, Lindon J

    2009-02-01

    The extended twin kinship design allows the simultaneous testing of additive and nonadditive genetic, shared and individual-specific environmental factors, as well as sex differences in the expression of genes and environment in the presence of assortative mating and combined genetic and cultural transmission (Eaves et al., 1999). It also handles the contribution of these sources of variance to the (co)variation of multiple phenotypes. Keller et al. (2008) extended this comprehensive model for family resemblance to allow or a flexible specification of assortment and vertical transmission. As such, it provides a general framework which can easily be reduced to fit subsets of data such as twin-parent data, children-of-twins data, etc. A flexible Mx specification of this model that allows handling of these various designs is presented in detail and applied to data from the Virginia 30,000. Data on height, body mass index, smoking status, church attendance, and political affiliation were obtained from twins and their families. Results indicate that biases in the estimation of variance components depend both on the types of relative available for analysis, and on the underlying genetic and environmental architecture of the phenotype of interest.

  19. Rational Design of Antirheumatic Prodrugs Specific for Sites of Inflammation

    PubMed Central

    Onuoha, Shimobi C.; Ferrari, Mathieu; Sblattero, Daniele

    2015-01-01

    Objective Biologic drugs, such as the anti–tumor necrosis factor (anti‐TNF) antibody adalimumab, have represented a breakthrough in the treatment of rheumatoid arthritis. Yet, concerns remain over their lack of efficacy in a sizable proportion of patients and their potential for systemic side effects such as infection. Improved biologic prodrugs specifically targeted to the site of inflammation have the potential to alleviate current concerns surrounding biologic anticytokine therapies. The purpose of this study was to design, construct, and evaluate in vitro and ex vivo the targeting and antiinflammatory capacity of activatable bispecific antibodies. Methods Activatable dual variable domain (aDVD) antibodies were designed and constructed to target intercellular adhesion molecule 1 (ICAM‐1), which is up‐regulated at sites of inflammation, and anti‐TNF antibodies (adalimumab and infliximab). These bispecific molecules included an external arm that targets ICAM‐1 and an internal arm that comprises the therapeutic domain of an anti‐TNF antibody. Both arms were linked to matrix metalloproteinase (MMP)–cleavable linkers. The constructs were tested for their ability to bind and neutralize both in vitro and ex vivo targets. Results Intact aDVD constructs demonstrated significantly reduced binding and anti‐TNF activity in the prodrug formulation as compared to the parent antibodies. Human synovial fluid and physiologic concentrations of MMP enzyme were capable of cleaving the external domain of the antibody, revealing a fully active molecule. Activated antibodies retained the same binding and anti‐TNF inhibitory capacities as the parent molecules. Conclusion The design of a biologic prodrug with enhanced specificity for sites of inflammation (synovium) and reduced specificity for off‐target TNF is described. This construct has the potential to form a platform technology that is capable of enhancing the therapeutic index of drugs for the treatment of

  20. High specific energy, high capacity nickel-hydrogen cell design

    NASA Technical Reports Server (NTRS)

    Wheeler, James R.

    1993-01-01

    A 3.5 inch rabbit-ear-terminal nickel-hydrogen cell was designed and tested to deliver high capacity at steady discharge rates up to and including a C rate. Its specific energy yield of 60.6 wh/kg is believed to be the highest yet achieved in a slurry-process nickel-hydrogen cell, and its 10 C capacity of 113.9 AH the highest capacity yet of any type in a 3.5 inch diameter size. The cell also demonstrated a pulse capability of 180 amps for 20 seconds. Specific cell parameters and performance are described. Also covered is an episode of capacity fading due to electrode swelling and its successful recovery by means of additional activation procedures.

  1. Advanced Wet Tantalum Capacitors: Design, Specifications and Performance

    NASA Technical Reports Server (NTRS)

    Teverovsky, Alexander

    2016-01-01

    Insertion of new types of commercial, high volumetric efficiency wet tantalum capacitors in space systems requires reassessment of the existing quality assurance approaches that have been developed for capacitors manufactured to MIL-PRF-39006 requirements. The specifics of wet electrolytic capacitors is that leakage currents flowing through electrolyte can cause gas generation resulting in building up of internal gas pressure and rupture of the case. The risk associated with excessive leakage currents and increased pressure is greater for high value advanced wet tantalum capacitors, but it has not been properly evaluated yet. This presentation gives a review of specifics of the design, performance, and potential reliability risks associated with advanced wet tantalum capacitors. Problems related to setting adequate requirements for DPA, leakage currents, hermeticity, stability at low and high temperatures, ripple currents for parts operating in vacuum, and random vibration testing are discussed. Recommendations for screening and qualification to reduce risks of failures have been suggested.

  2. Design and Evaluation of Tumor-Specific Dendrimer Epigenetic Therapeutics

    PubMed Central

    Zong, Hong; Shah, Dhavan; Selwa, Katherine; Tsuchida, Ryan E; Rattan, Rahul; Mohan, Jay; Stein, Adam B; Otis, James B; Goonewardena, Sascha N

    2015-01-01

    Histone deacetylase inhibitors (HDACi) are promising therapeutics for cancer. HDACi alter the epigenetic state of tumors and provide a unique approach to treat cancer. Although studies with HDACi have shown promise in some cancers, variable efficacy and off-target effects have limited their use. To overcome some of the challenges of traditional HDACi, we sought to use a tumor-specific dendrimer scaffold to deliver HDACi directly to cancer cells. Here we report the design and evaluation of tumor-specific dendrimer–HDACi conjugates. The HDACi was conjugated to the dendrimer using an ester linkage through its hydroxamic acid group, inactivating the HDACi until it is released from the dendrimer. Using a cancer cell model, we demonstrate the functionality of the tumor-specific dendrimer–HDACi conjugates. Furthermore, we demonstrate that unlike traditional HDACi, dendrimer–HDACi conjugates do not affect tumor-associated macrophages, a recently recognized mechanism through which drug resistance emerges. We anticipate that this new class of cell-specific epigenetic therapeutics will have tremendous potential in the treatment of cancer. PMID:26246996

  3. Integrating Cloud-Computing-Specific Model into Aircraft Design

    NASA Astrophysics Data System (ADS)

    Zhimin, Tian; Qi, Lin; Guangwen, Yang

    Cloud Computing is becoming increasingly relevant, as it will enable companies involved in spreading this technology to open the door to Web 3.0. In the paper, the new categories of services introduced will slowly replace many types of computational resources currently used. In this perspective, grid computing, the basic element for the large scale supply of cloud services, will play a fundamental role in defining how those services will be provided. The paper tries to integrate cloud computing specific model into aircraft design. This work has acquired good results in sharing licenses of large scale and expensive software, such as CFD (Computational Fluid Dynamics), UG, CATIA, and so on.

  4. Tissue-specific designs of stem cell hierarchies.

    PubMed

    Visvader, Jane E; Clevers, Hans

    2016-04-01

    Recent work in the field of stem cell biology suggests that there is no single design for an adult tissue stem cell hierarchy, and that different tissues employ distinct strategies to meet their self-renewal and repair requirements. Stem cells may be multipotent or unipotent, and can exist in quiescent or actively dividing states. 'Professional' stem cells may also co-exist with facultative stem cells, which are more specialized daughter cells that revert to a stem cell state under specific tissue damage conditions. Here, we discuss stem cell strategies as seen in three solid mammalian tissues: the intestine, mammary gland and skeletal muscle. PMID:26999737

  5. Modular Integrated Stackable Layers (MISL) 1.1 Design Specification. Design Guideline Document

    NASA Technical Reports Server (NTRS)

    Yim, Hester J.

    2012-01-01

    This document establishes the design guideline of the Modular Instrumentation Data Acquisition (MI-DAQ) system in utilization of several designs available in EV. The MI- DAQ provides the options to the customers depending on their system requirements i.e. a 28V interface power supply, a low power battery operated system, a low power microcontroller, a higher performance microcontroller, a USB interface, a Ethernet interface, a wireless communication, various sensor interfaces, etc. Depending on customer's requirements, the each functional board can be stacked up from a bottom level of power supply to a higher level of stack to provide user interfaces. The stack up of boards are accomplished by a predefined and standardized power bus and data bus connections which are included in this document along with other physical and electrical guidelines. This guideline also provides information for a new design options. This specification is the product of a collaboration between NASA/JSC/EV and Texas A&M University. The goal of the collaboration is to open source the specification and allow outside entities to design, build, and market modules that are compatible with the specification. NASA has designed and is using numerous modules that are compatible to this specification. A limited number of these modules will also be released as open source designs to support the collaboration. The released designs are listed in the Applicable Documents.

  6. 41 CFR 302-9.170 - Under what specific conditions may my agency authorize transportation of a POV to my post of duty...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... conditions may my agency authorize transportation of a POV to my post of duty subsequent to the time of my assignment to that post? 302-9.170 Section 302-9.170 Public Contracts and Property Management Federal Travel... the Time of Assignment § 302-9.170 Under what specific conditions may my agency...

  7. 41 CFR 302-9.170 - Under what specific conditions may my agency authorize transportation of a POV to my post of duty...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... conditions may my agency authorize transportation of a POV to my post of duty subsequent to the time of my assignment to that post? 302-9.170 Section 302-9.170 Public Contracts and Property Management Federal Travel... the Time of Assignment § 302-9.170 Under what specific conditions may my agency...

  8. 41 CFR 302-9.170 - Under what specific conditions may my agency authorize transportation of a POV to my post of duty...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... conditions may my agency authorize transportation of a POV to my post of duty subsequent to the time of my assignment to that post? 302-9.170 Section 302-9.170 Public Contracts and Property Management Federal Travel... Subsequent to the Time of Assignment § 302-9.170 Under what specific conditions may my agency...

  9. 41 CFR 302-9.170 - Under what specific conditions may my agency authorize transportation of a POV to my post of duty...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... conditions may my agency authorize transportation of a POV to my post of duty subsequent to the time of my assignment to that post? 302-9.170 Section 302-9.170 Public Contracts and Property Management Federal Travel... Subsequent to the Time of Assignment § 302-9.170 Under what specific conditions may my agency...

  10. 41 CFR 302-9.170 - Under what specific conditions may my agency authorize transportation of a POV to my post of duty...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... conditions may my agency authorize transportation of a POV to my post of duty subsequent to the time of my assignment to that post? 302-9.170 Section 302-9.170 Public Contracts and Property Management Federal Travel... Subsequent to the Time of Assignment § 302-9.170 Under what specific conditions may my agency...

  11. A drug-specific nanocarrier design for efficient anticancer therapy

    NASA Astrophysics Data System (ADS)

    Shi, Changying; Guo, Dandan; Xiao, Kai; Wang, Xu; Wang, Lili; Luo, Juntao

    2015-07-01

    The drug-loading properties of nanocarriers depend on the chemical structures and properties of their building blocks. Here we customize telodendrimers (linear dendritic copolymer) to design a nanocarrier with improved in vivo drug delivery characteristics. We do a virtual screen of a library of small molecules to identify the optimal building blocks for precise telodendrimer synthesis using peptide chemistry. With rationally designed telodendrimer architectures, we then optimize the drug-binding affinity of a nanocarrier by introducing an optimal drug-binding molecule (DBM) without sacrificing the stability of the nanocarrier. To validate the computational predictions, we synthesize a series of nanocarriers and evaluate systematically for doxorubicin delivery. Rhein-containing nanocarriers have sustained drug release, prolonged circulation, increased tolerated dose, reduced toxicity, effective tumour targeting and superior anticancer effects owing to favourable doxorubicin-binding affinity and improved nanoparticle stability. This study demonstrates the feasibility and versatility of the de novo design of telodendrimer nanocarriers for specific drug molecules, which is a promising approach to transform nanocarrier development for drug delivery.

  12. A drug-specific nanocarrier design for efficient anticancer therapy

    PubMed Central

    Shi, Changying; Guo, Dandan; Xiao, Kai; Wang, Xu; Wang, Lili; Luo, Juntao

    2015-01-01

    The drug-loading properties of nanocarriers depend on the chemical structures and properties of their building blocks. Here, we customize telodendrimers (linear-dendritic copolymer) to design a nanocarrier with improved in vivo drug delivery characteristics. We do a virtual screen of a library of small molecules to identify the optimal building blocks for precise telodendrimer synthesis using peptide chemistry. With rationally designed telodendrimer architectures, we then optimize the drug binding affinity of a nanocarrier by introducing an optimal drug-binding molecule (DBM) without sacrificing the stability of the nanocarrier. To validate the computational predictions, we synthesize a series of nanocarriers and evaluate systematically for doxorubicin delivery. Rhein-containing nanocarriers have sustained drug release, prolonged circulation, increased tolerated dose, reduced toxicity, effective tumor targeting and superior anticancer effects owing to favourable doxorubicin-binding affinity and improved nanoparticle stability. This study demonstrates the feasibility and versatility of the de novo design of telodendrimer nanocarriers for specific drug molecules, which is a promising approach to transform nanocarrier development for drug delivery. PMID:26158623

  13. Stochastic sensors designed for assessment of biomarkers specific to obesity.

    PubMed

    Cioates Negut, Catalina; Stefan-van Staden, Raluca-Ioana; Ungureanu, Eleonora-Mihaela; Udeanu, Denisa Ioana

    2016-09-01

    Two stochastic sensors based on the following oleamides: 1-adamantyloleamide and N,N-dimethyl-N-(2-oleylamidoethyl)amine physically immobilized on graphite paste were designed. The sensors were able to determine simultaneously from the whole blood of Wistar rats three biomarkers specific to obesity: leptin, interleukin-6 (IL-6) and plasminogen activator inhibitor 1 (PAI-1). The whole blood samples were obtained from Wistar rats treated with oleoylethanolamide (OEA), (Z)-N-[(1S)-2-hidroxy-1-(phenylmethyl) ethyl]-9octadecenamide (OLA), and with the aqueous solution of 1% Tween 80 used as solvent for oleamides formulations (control samples). The proposed sensors were very sensitive and reliable for the assay of obesity biomarkers in whole blood of rats. PMID:27288757

  14. Stochastic sensors designed for assessment of biomarkers specific to obesity.

    PubMed

    Cioates Negut, Catalina; Stefan-van Staden, Raluca-Ioana; Ungureanu, Eleonora-Mihaela; Udeanu, Denisa Ioana

    2016-09-01

    Two stochastic sensors based on the following oleamides: 1-adamantyloleamide and N,N-dimethyl-N-(2-oleylamidoethyl)amine physically immobilized on graphite paste were designed. The sensors were able to determine simultaneously from the whole blood of Wistar rats three biomarkers specific to obesity: leptin, interleukin-6 (IL-6) and plasminogen activator inhibitor 1 (PAI-1). The whole blood samples were obtained from Wistar rats treated with oleoylethanolamide (OEA), (Z)-N-[(1S)-2-hidroxy-1-(phenylmethyl) ethyl]-9octadecenamide (OLA), and with the aqueous solution of 1% Tween 80 used as solvent for oleamides formulations (control samples). The proposed sensors were very sensitive and reliable for the assay of obesity biomarkers in whole blood of rats.

  15. Novel Designs for Application Specific MEMS Pressure Sensors

    PubMed Central

    Fragiacomo, Giulio; Reck, Kasper; Lorenzen, Lasse; Thomsen, Erik V.

    2010-01-01

    In the framework of developing innovative microfabricated pressure sensors, we present here three designs based on different readout principles, each one tailored for a specific application. A touch mode capacitive pressure sensor with high sensitivity (14 pF/bar), low temperature dependence and high capacitive output signal (more than 100 pF) is depicted. An optical pressure sensor intrinsically immune to electromagnetic interference, with large pressure range (0–350 bar) and a sensitivity of 1 pm/bar is presented. Finally, a resonating wireless pressure sensor power source free with a sensitivity of 650 KHz/mmHg is described. These sensors will be related with their applications in harsh environment, distributed systems and medical environment, respectively. For many aspects, commercially available sensors, which in vast majority are piezoresistive, are not suited for the applications proposed. PMID:22163425

  16. An overview of field-specific designs of microbial EOR

    SciTech Connect

    Robertson, E.P.; Bala, G.A.; Fox, S.L.; Jackson, J.D.; Thomas, C.P.

    1995-12-31

    The selection and design of an MEOR process for application in a specific field involves geological, reservoir, and biological characterization. Microbially mediated oil recovery mechanisms (bigenic gas, biopolymers, and biosurfactants) are defined by the types of microorganisms used. The engineering and biological character of a given reservoir must be understood to correctly select a microbial system to enhance oil recovery. This paper discusses the methods used to evaluate three fields with distinct characteristics and production problems for the applicability of MEOR would not be applicable in two of the three fields considered. The development of a microbial oil recovery process for the third field appeared promising. Development of a bacterial consortium capable of producing the desired metabolites was initiated, and field isolates were characterized.

  17. An overview of field specific designs of microbial EOR

    SciTech Connect

    Robertson, E.P.; Bala, G.A.; Fox, S.L.; Jackson, J.D.; Thomas, C.P.

    1995-12-01

    The selection and design of a microbial enhanced oil recovery (MEOR) process for application in a specific field involves geological, reservoir, and biological characterization. Microbially mediated oil recovery mechanisms (biogenic gas, biopolymers, and biosurfactants) are defined by the types of microorganisms used. The engineering and biological character of a given reservoir must be understood to correctly select a microbial system to enhance oil recovery. The objective of this paper is to discuss the methods used to evaluate three fields with distinct characteristics and production problems for the applicability of MEOR technology. Reservoir characteristics and laboratory results indicated that MEOR would not be applicable in two of the three fields considered. The development of a microbial oil recovery process for the third field appeared promising. Development of a bacterial consortium capable of producing the desired metabolites was initiated and field isolates were characterized.

  18. Geometric design of mechanical linkages for contact specifications

    NASA Astrophysics Data System (ADS)

    Robson, Nina Patarinsky

    2008-10-01

    This dissertation focuses on the kinematic synthesis of mechanical linkages in order to guide an end-effortor so that it maintains contact with specified objects in its workspace. Assuming the serial chain does not have full mobility in its workspace, the contact geometry is used to determine the dimensions of the serial chain. The approach to this problem, is to use the relative curvature of the contact of the end-effector with one or more objects to define velocity and acceleration specifications for its movement. This provides kinematic constraints that are used to synthesize the dimensions of the serial chain. The mathematical formulation of the geometric design problem, leads to systems of multivariable polynomial equations, which are solved exactly using sparse matrix resultants and polynomial homotopy methods. The results from this research yield planar RR and 4R linkages that match a specified contact geometry, spatial TS, parallel RRS and perpendicular RRS linkages that have a required acceleration specification. A new strategy for a robot recovery from actuator failures is demonstrated for the Mars Exploratory Rover Arm. In extending this work to spatial serial chains, a new method based on sparse matrix resultants was developed, which solves exact synthesis problems with acceleration constraints. Further the research builds on the theoretical concepts of contact relationships for spatial movement. The connection between kinematic synthesis and contact problems and its extension to spatial synthesis are developed in this dissertation for the first time and are new contributions. The results, which rely upon the use of surface curvature effects to reduce the number of fixtures needed to immobilize an object, find applications in robot grasping and part-fixturing. The recovery strategy, presented in this research is also a new concept. The recognition that it is possible to reconfigure a crippled robotic system to achieve mission critical tasks can guide

  19. DITDOS: A set of design specifications for distributed data inventories

    NASA Technical Reports Server (NTRS)

    King, T. A.; Walker, R. J.; Joy, S. P.

    1995-01-01

    The analysis of space science data often requires researchers to work with many different types of data. For instance, correlative analysis can require data from multiple instruments on a single spacecraft, multiple spacecraft, and ground-based data. Typically, data from each source are available in a different format and have been written on a different type of computer, and so much effort must be spent to read the data and convert it to the computer and format that the researchers use in their analysis. The large and ever-growing amount of data and the large investment by the scientific community in software that require a specific data format make using standard data formats impractical. A format-independent approach to accessing and analyzing disparate data is key to being able to deliver data to a diverse community in a timely fashion. The system in use at the Planetary Plasma Interactions (PPI) node of the NASA Planetary Data System (PDS) is based on the object-oriented Distributed Inventory Tracking and Data Ordering Specification (DITDOS), which describes data inventories in a storage independent way. The specifications have been designed to make it possible to build DITDOS compliant inventories that can exist on portable media such as CD-ROM's. The portable media can be moved within a system, or from system to system, and still be used without modification. Several applications have been developed to work with DITDOS compliant data holdings. One is a windows-based client/server application, which helps guide the user in the selection of data. A user can select a data base, then a data set, then a specific data file, and then either order the data and receive it immediately if it is online or request that it be brought online if it is not. A user can also view data by any of the supported methods. DITDOS makes it possible to use already existing applications for data-specific actions, and this is done whenever possible. Another application is a stand

  20. 41 CFR 102-74.340 - Who is responsible for monitoring and controlling areas designated for smoking by an agency head...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... monitoring and controlling areas designated for smoking by an agency head and for identifying those areas... MANAGEMENT Facility Management Smoking § 102-74.340 Who is responsible for monitoring and controlling areas designated for smoking by an agency head and for identifying those areas with proper signage? Agency...

  1. 41 CFR 102-74.340 - Who is responsible for monitoring and controlling areas designated for smoking by an agency head...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... monitoring and controlling areas designated for smoking by an agency head and for identifying those areas... MANAGEMENT Facility Management Smoking § 102-74.340 Who is responsible for monitoring and controlling areas designated for smoking by an agency head and for identifying those areas with proper signage? Agency...

  2. 41 CFR 102-74.340 - Who is responsible for monitoring and controlling areas designated for smoking by an agency head...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... monitoring and controlling areas designated for smoking by an agency head and for identifying those areas... MANAGEMENT Facility Management Smoking § 102-74.340 Who is responsible for monitoring and controlling areas designated for smoking by an agency head and for identifying those areas with proper signage? Agency...

  3. 41 CFR 102-74.340 - Who is responsible for monitoring and controlling areas designated for smoking by an agency head...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... monitoring and controlling areas designated for smoking by an agency head and for identifying those areas... MANAGEMENT Facility Management Smoking § 102-74.340 Who is responsible for monitoring and controlling areas designated for smoking by an agency head and for identifying those areas with proper signage? Agency...

  4. Should dialysis modalities be designed to remove specific uremic toxins?

    PubMed

    Baurmeister, Ulrich; Vienken, Joerg; Ward, Richard A

    2009-01-01

    The definition of optimal dialysis therapy remains elusive. Randomized clinical trials have neither supported using urea as a surrogate marker for uremic toxicity nor provided clear cut evidence in favor of larger solutes. Thus, where to focus resources in the development of new membranes, and therapies remains unclear. Three basic questions remain unanswered: (i) what solute(s) should be used as a marker for optimal dialysis; (ii) should dialytic therapies be designed to remove a specific solute; and (iii) how can current therapies be modified to provide better control of uremic toxicity? Identification of a single, well-defined uremic toxin appears to be unlikely as new analytical tools reveal an increasingly complex uremic milieu. As a result, it is probable that membranes and therapies should be designed for the nonspecific removal of a wide variety of solutes retained in uremia. Removal of the widest range of solutes can best be achieved using existing therapies that incorporate convection in conjunction with longer treatment times and more frequent treatments. Membranes capable of removing solutes over an expanded effective molecular size range can already be fabricated; however, their use will require novel approaches to conserve proteins, such as albumin.

  5. Measurement of Spray Drift with a Specifically Designed Lidar System.

    PubMed

    Gregorio, Eduard; Torrent, Xavier; Planas de Martí, Santiago; Solanelles, Francesc; Sanz, Ricardo; Rocadenbosch, Francesc; Masip, Joan; Ribes-Dasi, Manel; Rosell-Polo, Joan R

    2016-01-01

    Field measurements of spray drift are usually carried out by passive collectors and tracers. However, these methods are labour- and time-intensive and only provide point- and time-integrated measurements. Unlike these methods, the light detection and ranging (lidar) technique allows real-time measurements, obtaining information with temporal and spatial resolution. Recently, the authors have developed the first eye-safe lidar system specifically designed for spray drift monitoring. This prototype is based on a 1534 nm erbium-doped glass laser and an 80 mm diameter telescope, has scanning capability, and is easily transportable. This paper presents the results of the first experimental campaign carried out with this instrument. High coefficients of determination (R² > 0.85) were observed by comparing lidar measurements of the spray drift with those obtained by horizontal collectors. Furthermore, the lidar system allowed an assessment of the drift reduction potential (DRP) when comparing low-drift nozzles with standard ones, resulting in a DRP of 57% (preliminary result) for the tested nozzles. The lidar system was also used for monitoring the evolution of the spray flux over the canopy and to generate 2-D images of these plumes. The developed instrument is an advantageous alternative to passive collectors and opens the possibility of new methods for field measurement of spray drift. PMID:27070613

  6. Measurement of Spray Drift with a Specifically Designed Lidar System

    PubMed Central

    Gregorio, Eduard; Torrent, Xavier; Planas de Martí, Santiago; Solanelles, Francesc; Sanz, Ricardo; Rocadenbosch, Francesc; Masip, Joan; Ribes-Dasi, Manel; Rosell-Polo, Joan R.

    2016-01-01

    Field measurements of spray drift are usually carried out by passive collectors and tracers. However, these methods are labour- and time-intensive and only provide point- and time-integrated measurements. Unlike these methods, the light detection and ranging (lidar) technique allows real-time measurements, obtaining information with temporal and spatial resolution. Recently, the authors have developed the first eye-safe lidar system specifically designed for spray drift monitoring. This prototype is based on a 1534 nm erbium-doped glass laser and an 80 mm diameter telescope, has scanning capability, and is easily transportable. This paper presents the results of the first experimental campaign carried out with this instrument. High coefficients of determination (R2 > 0.85) were observed by comparing lidar measurements of the spray drift with those obtained by horizontal collectors. Furthermore, the lidar system allowed an assessment of the drift reduction potential (DRP) when comparing low-drift nozzles with standard ones, resulting in a DRP of 57% (preliminary result) for the tested nozzles. The lidar system was also used for monitoring the evolution of the spray flux over the canopy and to generate 2-D images of these plumes. The developed instrument is an advantageous alternative to passive collectors and opens the possibility of new methods for field measurement of spray drift. PMID:27070613

  7. Measurement of Spray Drift with a Specifically Designed Lidar System.

    PubMed

    Gregorio, Eduard; Torrent, Xavier; Planas de Martí, Santiago; Solanelles, Francesc; Sanz, Ricardo; Rocadenbosch, Francesc; Masip, Joan; Ribes-Dasi, Manel; Rosell-Polo, Joan R

    2016-04-08

    Field measurements of spray drift are usually carried out by passive collectors and tracers. However, these methods are labour- and time-intensive and only provide point- and time-integrated measurements. Unlike these methods, the light detection and ranging (lidar) technique allows real-time measurements, obtaining information with temporal and spatial resolution. Recently, the authors have developed the first eye-safe lidar system specifically designed for spray drift monitoring. This prototype is based on a 1534 nm erbium-doped glass laser and an 80 mm diameter telescope, has scanning capability, and is easily transportable. This paper presents the results of the first experimental campaign carried out with this instrument. High coefficients of determination (R² > 0.85) were observed by comparing lidar measurements of the spray drift with those obtained by horizontal collectors. Furthermore, the lidar system allowed an assessment of the drift reduction potential (DRP) when comparing low-drift nozzles with standard ones, resulting in a DRP of 57% (preliminary result) for the tested nozzles. The lidar system was also used for monitoring the evolution of the spray flux over the canopy and to generate 2-D images of these plumes. The developed instrument is an advantageous alternative to passive collectors and opens the possibility of new methods for field measurement of spray drift.

  8. Space Station Cathode Design, Performance, and Operating Specifications

    NASA Technical Reports Server (NTRS)

    Patterson, Michael J.; Verhey, Timothy R.; Soulas, George; Zakany, James

    1998-01-01

    A plasma contactor system was baselined for the International Space Station (ISS) to eliminate/mitigate damaging interactions with the space environment. The system represents a dual-use technology which is a direct outgrowth of the NASA electric propulsion program and, in particular, the technology development efforts on ion thruster systems. The plasma contactor includes a hollow cathode assembly (HCA), a power electronics unit, and a xenon gas feed system. Under a pre-flight development program, these subsystems were taken to the level of maturity appropriate for transfer to U.S. industry for final development. NASA's Lewis Research Center was subsequently requested by ISS to manufacture and deliver the engineering model, qualification model, and flight HCA units. To date, multiple units have been built. One cathode has demonstrated approximately 28,000 hours lifetime, two development unit HCAs have demonstrated over 10,000 hours lifetime, and one development unit HCA has demonstrated more than 32,000 ignitions. All 8 flight HCAs have been manufactured, acceptance tested, and are ready for delivery to the flight contractor. This paper discusses the requirements, mechanical design, performance, operating specifications, and schedule for the plasma contactor flight HCAs.

  9. 41 CFR 302-9.140 - Under what specific conditions may my agency authorize transportation of a POV to my post of duty...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 41 Public Contracts and Property Management 4 2013-07-01 2012-07-01 true Under what specific... of duty? 302-9.140 Section 302-9.140 Public Contracts and Property Management Federal Travel... at Time of Assignment § 302-9.140 Under what specific conditions may my agency...

  10. 41 CFR 302-9.140 - Under what specific conditions may my agency authorize transportation of a POV to my post of duty...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 41 Public Contracts and Property Management 4 2012-07-01 2012-07-01 false Under what specific... of duty? 302-9.140 Section 302-9.140 Public Contracts and Property Management Federal Travel... at Time of Assignment § 302-9.140 Under what specific conditions may my agency...

  11. 41 CFR 302-9.140 - Under what specific conditions may my agency authorize transportation of a POV to my post of duty...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 41 Public Contracts and Property Management 4 2014-07-01 2014-07-01 false Under what specific... of duty? 302-9.140 Section 302-9.140 Public Contracts and Property Management Federal Travel... at Time of Assignment § 302-9.140 Under what specific conditions may my agency...

  12. 49 CFR 107.402 - Application for designation as a certification agency.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... be approved as a certification agency must apply in writing to the Associate Administrator for... fireworks or explosives and a degree in the physical sciences or engineering from an accredited university... been met; (iii) Written operating procedures to be used by the Fireworks Certification Agency to...

  13. 78 FR 14586 - Agency Information Collection Activities: Application for Civil Surgeon Designation Registration...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-06

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Application.... Citizenship and Immigration Services (USCIS) invites the general public and other Federal agencies to comment... Policy and Strategy, U.S. Citizenship and Immigration Services, Department of Homeland Security....

  14. 34 CFR 370.41 - What conflict of interest provision applies to employees of a designated agency?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... of Education (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION CLIENT ASSISTANCE PROGRAM What Post-Award Conditions Must Be Met by a Designated Agency? § 370.41... the Act in the State; or (2) Provide any services under the Act, other than CAP and PAIR services....

  15. 78 FR 51732 - The Food and Drug Administration/European Medicines Agency Orphan Product Designation and Grant...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-21

    ... valuable information about the FDA and European Medicines Agency (EMA) Orphan Drug Designation programs.... For parking and security information, please refer to http://www.fda.gov/AboutFDA/WorkingatFDA/BuildingsandFacilities/WhiteOakCampusInformation/ucm241740.htm . Contact Person: Eleanor Dixon-Terry, Food...

  16. 41 CFR 102-76.55 - What sustainable development principles must Federal agencies apply to the siting, design, and...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... and Construction Sustainable Development § 102-76.55 What sustainable development principles must... Acquisition,” Federal agencies must apply sustainable development principles to the siting, design, and... 41 Public Contracts and Property Management 3 2012-01-01 2012-01-01 false What...

  17. 41 CFR 102-76.55 - What sustainable development principles must Federal agencies apply to the siting, design, and...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... and Construction Sustainable Development § 102-76.55 What sustainable development principles must... Acquisition,” Federal agencies must apply sustainable development principles to the siting, design, and... 41 Public Contracts and Property Management 3 2013-07-01 2013-07-01 false What...

  18. 41 CFR 102-76.55 - What sustainable development principles must Federal agencies apply to the siting, design, and...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... and Construction Sustainable Development § 102-76.55 What sustainable development principles must... Acquisition,” Federal agencies must apply sustainable development principles to the siting, design, and... 41 Public Contracts and Property Management 3 2014-01-01 2014-01-01 false What...

  19. 41 CFR 102-76.55 - What sustainable development principles must Federal agencies apply to the siting, design, and...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... and Construction Sustainable Development § 102-76.55 What sustainable development principles must... Acquisition,” Federal agencies must apply sustainable development principles to the siting, design, and... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false What...

  20. Design specifications for manufacturability of MCM-C multichip modules

    SciTech Connect

    Blazek, R.; Desch, J.; Kautz, D.; Morgenstern, H.

    1996-10-01

    A comprehensive guide for ceramic-based multichip modules (MCMS) has been developed by AlliedSignal Federal Manufacturing & Technologies (FM&T) to provide manufacturability information for its customers about how MCM designs can be affected by existing process and equipment capabilities. This guide extends beyond a listing of design rules by providing information about design layout, low- temperature cofired ceramic (LTCC) substrate fabrication, MCM assembly and electrical testing Electrical mechanical packaging, environmental, and producibility issues are reviewed. Examples of three MCM designs are shown in the form of packaging cross-sectional views, LTCC substrate layer allocations, and overall MCM photographs. The guide has proven to be an effective tool for enhancing communications between MCM designers and manufacturers and producing a microcircuit that meets design requirements within the limitations of process capabilities.

  1. 45 CFR 90.43 - What specific responsibilities do agencies and recipients have to ensure compliance with the Act?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...? (a) Written notice, technical assistance, and educational materials. Each agency shall: (1) Provide..., where necessary, to recipients to aid them in complying with the Act. (3) Make available educational...-evaluation. (1) Each agency shall require each recipient employing the equivalent of 15 or more full...

  2. A requirements specification for a software design support system

    NASA Technical Reports Server (NTRS)

    Noonan, Robert E.

    1988-01-01

    Most existing software design systems (SDSS) support the use of only a single design methodology. A good SDSS should support a wide variety of design methods and languages including structured design, object-oriented design, and finite state machines. It might seem that a multiparadigm SDSS would be expensive in both time and money to construct. However, it is proposed that instead an extensible SDSS that directly implements only minimal database and graphical facilities be constructed. In particular, it should not directly implement tools to faciliate language definition and analysis. It is believed that such a system could be rapidly developed and put into limited production use, with the experience gained used to refine and evolve the systems over time.

  3. Medicare, Medicaid, and Children's Health Insurance Programs: announcement of the extended temporary moratoria on enrollment of ambulance suppliers and home health agencies in designated geographic locations. Extension of temporary moratoria.

    PubMed

    2014-08-01

    This document announces the extension of temporary moratoria on the enrollment of new ambulance suppliers and home health agencies (HHAs) in specific locations within designated metropolitan areas in Florida, Illinois, Michigan, Texas, Pennsylvania, and New Jersey to prevent and combat fraud, waste, and abuse.

  4. NASA software specification and evaluation system design, part 1

    NASA Technical Reports Server (NTRS)

    1976-01-01

    The research to develop methods for reducing the effort expended in software and verification is reported. The development of a formal software requirements methodology, a formal specifications language, a programming language, a language preprocessor, and code analysis tools are discussed.

  5. Reprogramming homing endonuclease specificity through computational design and directed evolution.

    PubMed

    Thyme, Summer B; Boissel, Sandrine J S; Arshiya Quadri, S; Nolan, Tony; Baker, Dean A; Park, Rachel U; Kusak, Lara; Ashworth, Justin; Baker, David

    2014-02-01

    Homing endonucleases (HEs) can be used to induce targeted genome modification to reduce the fitness of pathogen vectors such as the malaria-transmitting Anopheles gambiae and to correct deleterious mutations in genetic diseases. We describe the creation of an extensive set of HE variants with novel DNA cleavage specificities using an integrated experimental and computational approach. Using computational modeling and an improved selection strategy, which optimizes specificity in addition to activity, we engineered an endonuclease to cleave in a gene associated with Anopheles sterility and another to cleave near a mutation that causes pyruvate kinase deficiency. In the course of this work we observed unanticipated context-dependence between bases which will need to be mechanistically understood for reprogramming of specificity to succeed more generally. PMID:24270794

  6. Formal design specification of a Processor Interface Unit

    NASA Technical Reports Server (NTRS)

    Fura, David A.; Windley, Phillip J.; Cohen, Gerald C.

    1992-01-01

    This report describes work to formally specify the requirements and design of a processor interface unit (PIU), a single-chip subsystem providing memory-interface bus-interface, and additional support services for a commercial microprocessor within a fault-tolerant computer system. This system, the Fault-Tolerant Embedded Processor (FTEP), is targeted towards applications in avionics and space requiring extremely high levels of mission reliability, extended maintenance-free operation, or both. The need for high-quality design assurance in such applications is an undisputed fact, given the disastrous consequences that even a single design flaw can produce. Thus, the further development and application of formal methods to fault-tolerant systems is of critical importance as these systems see increasing use in modern society.

  7. Specifications for a COM Catalog Designed for Government Documents.

    ERIC Educational Resources Information Center

    Copeland, Nora S.; And Others

    Prepared in MARC format in accordance with the Ohio College Library Center (OCLC) standards, these specifications were developed at Colorado State University to catalog a group of government publications not listed in the Monthly Catalog of United States Publications. The resulting microfiche catalog produced through the OCLC Cataloging Subsystem…

  8. Languages for Specific Purposes. Program Design and Evaluation.

    ERIC Educational Resources Information Center

    Mackay, Ronald, Ed.; Palmer, Joe Darwin, Ed.

    This collection of research on curriculum and program development in languages for special purposes (LSP) contains the following papers: (1) "LSP Curriculum Development--From Policy to Practice," by Ronald Mackay and Maryse Bosquet; (2) "The Problem of Needs Assessment in English for Specific Purposes: Some Theoretical and Practical…

  9. As-built design specification for segment map (Sgmap) program

    NASA Technical Reports Server (NTRS)

    Tompkins, M. A. (Principal Investigator)

    1981-01-01

    The segment map program (SGMAP), which is part of the CLASFYT package, is described in detail. This program is designed to output symbolic maps or numerical dumps from LANDSAT cluster/classification files or aircraft ground truth/processed ground truth files which are in 'universal' format.

  10. 46 CFR 162.050-25 - Cargo monitor: Design specification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... in an explosive atmosphere must be approved by an independent laboratory as components that... cause formation of static electricity. (e) A monitor must be designed to operate in each plane that... warning signal and a signal that can be used to actuate valves in a vessel's fixed piping system, when—...

  11. 46 CFR 162.050-25 - Cargo monitor: Design specification.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... in an explosive atmosphere must be approved by an independent laboratory as components that... cause formation of static electricity. (e) A monitor must be designed to operate in each plane that... warning signal and a signal that can be used to actuate valves in a vessel's fixed piping system, when—...

  12. 46 CFR 162.050-25 - Cargo monitor: Design specification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... in an explosive atmosphere must be approved by an independent laboratory as components that... cause formation of static electricity. (e) A monitor must be designed to operate in each plane that... warning signal and a signal that can be used to actuate valves in a vessel's fixed piping system, when—...

  13. 46 CFR 162.050-25 - Cargo monitor: Design specification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... in an explosive atmosphere must be approved by an independent laboratory as components that... cause formation of static electricity. (e) A monitor must be designed to operate in each plane that... warning signal and a signal that can be used to actuate valves in a vessel's fixed piping system, when—...

  14. 46 CFR 162.050-25 - Cargo monitor: Design specification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... in an explosive atmosphere must be approved by an independent laboratory as components that... cause formation of static electricity. (e) A monitor must be designed to operate in each plane that... warning signal and a signal that can be used to actuate valves in a vessel's fixed piping system, when—...

  15. 41 CFR 102-76.25 - What standards must Federal agencies meet in providing architectural and interior design services?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design and Construction § 102-76.25... accordance with the standards in § 102-76.65. (c) Use metric specifications in construction where the metric... practical. (d) Provide for the design of security systems to protect Federal workers and visitors and...

  16. 41 CFR 102-76.25 - What standards must Federal agencies meet in providing architectural and interior design services?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design and Construction § 102-76.25... accordance with the standards in § 102-76.65. (c) Use metric specifications in construction where the metric... practical. (d) Provide for the design of security systems to protect Federal workers and visitors and...

  17. 41 CFR 102-76.25 - What standards must Federal agencies meet in providing architectural and interior design services?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design and Construction § 102-76.25... accordance with the standards in § 102-76.65. (c) Use metric specifications in construction where the metric... practical. (d) Provide for the design of security systems to protect Federal workers and visitors and...

  18. 5 CFR 5501.102 - Designation of HHS components as separate agencies.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Institutes of Health; (11) Program Support Center; and (12) Substance Abuse and Mental Health Services... agencies. 5501.102 Section 5501.102 Administrative Personnel DEPARTMENT OF HEALTH AND HUMAN SERVICES SUPPLEMENTAL STANDARDS OF ETHICAL CONDUCT FOR EMPLOYEES OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES §...

  19. 78 FR 39734 - Agency Information Collection Activities; Proposed Collection; Comment Request; Designated New...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-02

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration Agency Information Collection Activities; Proposed... Species (MUMS) Animal Health Act of 2004 amended the Federal Food, Drug, and Cosmetic Act (the FD&C...

  20. 44 CFR 60.25 - Designation, duties, and responsibilities of State Coordinating Agencies.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY INSURANCE AND HAZARD MITIGATION National Flood... this part and in conformance with other Federal and State environmental and water pollution standards for the prevention of pollution during periods of flooding; (10) Assure coordination and...

  1. 44 CFR 60.25 - Designation, duties, and responsibilities of State Coordinating Agencies.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY INSURANCE AND HAZARD MITIGATION National Flood... this part and in conformance with other Federal and State environmental and water pollution standards for the prevention of pollution during periods of flooding; (10) Assure coordination and...

  2. 44 CFR 60.25 - Designation, duties, and responsibilities of State Coordinating Agencies.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY INSURANCE AND HAZARD MITIGATION National Flood... this part and in conformance with other Federal and State environmental and water pollution standards for the prevention of pollution during periods of flooding; (10) Assure coordination and...

  3. 44 CFR 60.25 - Designation, duties, and responsibilities of State Coordinating Agencies.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY INSURANCE AND HAZARD MITIGATION National Flood... this part and in conformance with other Federal and State environmental and water pollution standards for the prevention of pollution during periods of flooding; (10) Assure coordination and...

  4. 44 CFR 60.25 - Designation, duties, and responsibilities of State Coordinating Agencies.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY INSURANCE AND HAZARD MITIGATION National Flood... this part and in conformance with other Federal and State environmental and water pollution standards for the prevention of pollution during periods of flooding; (10) Assure coordination and...

  5. 5 CFR 5501.102 - Designation of HHS components as separate agencies.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Institutes of Health; (11) Program Support Center; and (12) Substance Abuse and Mental Health Services... agencies. 5501.102 Section 5501.102 Administrative Personnel DEPARTMENT OF HEALTH AND HUMAN SERVICES SUPPLEMENTAL STANDARDS OF ETHICAL CONDUCT FOR EMPLOYEES OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES §...

  6. 5 CFR 5501.102 - Designation of HHS components as separate agencies.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Institutes of Health; (11) Program Support Center; and (12) Substance Abuse and Mental Health Services... agencies. 5501.102 Section 5501.102 Administrative Personnel DEPARTMENT OF HEALTH AND HUMAN SERVICES SUPPLEMENTAL STANDARDS OF ETHICAL CONDUCT FOR EMPLOYEES OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES §...

  7. 5 CFR 5501.102 - Designation of HHS components as separate agencies.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Institutes of Health; (11) Program Support Center; and (12) Substance Abuse and Mental Health Services... agencies. 5501.102 Section 5501.102 Administrative Personnel DEPARTMENT OF HEALTH AND HUMAN SERVICES SUPPLEMENTAL STANDARDS OF ETHICAL CONDUCT FOR EMPLOYEES OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES §...

  8. NASIS data base management system: IBM 360 TSS implementation. Volume 4: Program design specifications

    NASA Technical Reports Server (NTRS)

    1973-01-01

    The design specifications for the programs and modules within the NASA Aerospace Safety Information System (NASIS) are presented. The purpose of the design specifications is to standardize the preparation of the specifications and to guide the program design. Each major functional module within the system is a separate entity for documentation purposes. The design specifications contain a description of, and specifications for, all detail processing which occurs in the module. Sub-models, reference tables, and data sets which are common to several modules are documented separately.

  9. NASIS data base management system - IBM 360/370 OS MVT implementation. 4: Program design specifications

    NASA Technical Reports Server (NTRS)

    1973-01-01

    The design specifications for the programs and modules within the NASA Aerospace Safety Information System (NASIS) are presented. The purpose of the design specifications is to standardize the preparation of the specifications and to guide the program design. Each major functional module within the system is a separate entity for documentation purposes. The design specifications contain a description of, and specifications for, all detail processing which occurs in the module. Sub-modules, reference tables, and data sets which are common to several modules are documented separately.

  10. Septa design for a prostate specific PET camera

    SciTech Connect

    Qi, Jinyi; Huber, Jennifer S.; Huesman, Ronald H.; Moses, William W.; Derenzo, Stephen E.; Budinger, Thomas F.

    2003-11-15

    The recent development of new prostate tracers has motivated us to build a low cost PET camera optimized to image the prostate. Coincidence imaging of positron emitters is achieved using a pair of external curved detector banks. The bottom bank is fixed below the patient bed, and the top bank moves upward for patient access and downward for maximum sensitivity. In this paper, we study the design of septa for the prostate camera using Monte Carlo simulations. The system performance is measured by the detectability of a prostate lesion. We have studied 17 septa configurations. The results show that the design of septa has a large impact on the lesion detection at a given activity concentration. Significant differences are also observed between the lesion detectability and the conventional noise equivalent count (NEC) performance, indicating that the NEC is not appropriate for the detection task.

  11. A review of design issues specific to hypersonic flight vehicles

    NASA Astrophysics Data System (ADS)

    Sziroczak, D.; Smith, H.

    2016-07-01

    This paper provides an overview of the current technical issues and challenges associated with the design of hypersonic vehicles. Two distinct classes of vehicles are reviewed; Hypersonic Transports and Space Launchers, their common features and differences are examined. After a brief historical overview, the paper takes a multi-disciplinary approach to these vehicles, discusses various design aspects, and technical challenges. Operational issues are explored, including mission profiles, current and predicted markets, in addition to environmental effects and human factors. Technological issues are also reviewed, focusing on the three major challenge areas associated with these vehicles: aerothermodynamics, propulsion, and structures. In addition, matters of reliability and maintainability are also presented. The paper also reviews the certification and flight testing of these vehicles from a global perspective. Finally the current stakeholders in the field of hypersonic flight are presented, summarizing the active programs and promising concepts.

  12. 14 CFR 91.705 - Operations within airspace designated as Minimum Navigation Performance Specification Airspace.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Minimum Navigation Performance Specification Airspace. 91.705 Section 91.705 Aeronautics and Space FEDERAL... Operations within airspace designated as Minimum Navigation Performance Specification Airspace. (a) Except as... airspace designated as Minimum Navigation Performance Specifications airspace unless— (1) The aircraft...

  13. 48 CFR 2052.227-70 - Drawings, designs, specifications, and other data.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Drawings, designs... Clauses 2052.227-70 Drawings, designs, specifications, and other data. As prescribed at 2027.305-70, the..., designs, specifications, and other data will be developed and the NRC must retain full rights to...

  14. Restraint system design and evaluation for military specific applications

    NASA Astrophysics Data System (ADS)

    Karwaczynski, Sebastian

    This research focuses on designing an optimal restraint system for usage in a military vehicle applications. The designed restraint system must accommodate a wide range of DHM's and ATD's with and without PPE such as: helmet, boots, and body armor. The evaluation of the restraint systems were conducted in a simulated vehicle environment, which was utilized to downselect the ideal restraint system for this program. In December of 2011 the OCP TECD program was formulated to increase occupant protection. To do this, 3D computer models were created to accommodate the entire Soldier population in the Army. These models included the entire PPE, which were later utilized for space claim activities and for designing new seats and restraints, which would accommodate them. Additionally, guidelines to increase protection levels while providing optimal comfort to the Soldier were created. The current and emerging threats were evaluated and focused on at the time of the program inception. Throughout this program various activities were conducted for restraint downselection including Soldier evaluations of various restraint system configurations. The Soldiers were given an opportunity to evaluate each system in a representative seat, which allowed them to position themselves in a manner consistent with the mission requirements. Systems ranged from fully automated to manual adjustment type systems. An evaluation of each particular system was conducted and analyzed against the other systems. It was discovered that the restraint systems, which utilize retractors allowed for automatic webbing stowage and allowed for easier access and repeatability when donning and doffing the restraint. It was also found that when an aid was introduced to help the Soldier don the restraint, it was more likely that such system would be utilized. Restraints were evaluated in drop tower experiments in addition to actual blast tests. An evaluation with this amount of detail had not been attempted

  15. Advanced Free Flight Planner and Dispatcher's Workstation: Preliminary Design Specification

    NASA Technical Reports Server (NTRS)

    Wilson, J.; Wright, C.; Couluris, G. J.

    1997-01-01

    The National Aeronautics and Space Administration (NASA) has implemented the Advanced Air Transportation Technology (AATT) program to investigate future improvements to the national and international air traffic management systems. This research, as part of the AATT program, developed preliminary design requirements for an advanced Airline Operations Control (AOC) dispatcher's workstation, with emphasis on flight planning. This design will support the implementation of an experimental workstation in NASA laboratories that would emulate AOC dispatch operations. The work developed an airline flight plan data base and specified requirements for: a computer tool for generation and evaluation of free flight, user preferred trajectories (UPT); the kernel of an advanced flight planning system to be incorporated into the UPT-generation tool; and an AOC workstation to house the UPT-generation tool and to provide a real-time testing environment. A prototype for the advanced flight plan optimization kernel was developed and demonstrated. The flight planner uses dynamic programming to search a four-dimensional wind and temperature grid to identify the optimal route, altitude and speed for successive segments of a flight. An iterative process is employed in which a series of trajectories are successively refined until the LTPT is identified. The flight planner is designed to function in the current operational environment as well as in free flight. The free flight environment would enable greater flexibility in UPT selection based on alleviation of current procedural constraints. The prototype also takes advantage of advanced computer processing capabilities to implement more powerful optimization routines than would be possible with older computer systems.

  16. Home Care Nursing via Computer Networks: Justification and Design Specifications

    PubMed Central

    Brennan, Patricia Flatley

    1988-01-01

    High-tech home care includes the use of information technologies, such as computer networks, to provide direct care to patients in the home. This paper presents the justification and design of a project using a free, public access computer network to deliver home care nursing. The intervention attempts to reduce isolation and improve problem solving among home care patients and their informal caregivers. Three modules comprise the intervention: a decision module, a communications module, and an information data base. This paper describes the experimental evaluation of the project, and discusses issues in the delivery of nursing care via computers.

  17. Rational Design of CXCR4 Specific Antibodies with Elongated CDRs

    PubMed Central

    2015-01-01

    The bovine antibody (BLV1H12) which has an ultralong heavy chain complementarity determining region 3 (CDRH3) provides a novel scaffold for antibody engineering. By substituting the extended CDRH3 of BLV1H12 with modified CXCR4 binding peptides that adopt a β-hairpin conformation, we generated antibodies specifically targeting the ligand binding pocket of CXCR4 receptor. These engineered antibodies selectively bind to CXCR4 expressing cells with binding affinities in the low nanomolar range. In addition, they inhibit SDF-1-dependent signal transduction and cell migration in a transwell assay. Finally, we also demonstrate that a similar strategy can be applied to other CDRs and show that a CDRH2-peptide fusion binds CXCR4 with a Kd of 0.9 nM. This work illustrates the versatility of scaffold-based antibody engineering and could greatly expand the antibody functional repertoire in the future. PMID:25041362

  18. Heat pipe design handbook, part 2. [digital computer code specifications

    NASA Technical Reports Server (NTRS)

    Skrabek, E. A.

    1972-01-01

    The utilization of a digital computer code for heat pipe analysis and design (HPAD) is described which calculates the steady state hydrodynamic heat transport capability of a heat pipe with a particular wick configuration, the working fluid being a function of wick cross-sectional area. Heat load, orientation, operating temperature, and heat pipe geometry are specified. Both one 'g' and zero 'g' environments are considered, and, at the user's option, the code will also perform a weight analysis and will calculate heat pipe temperature drops. The central porous slab, circumferential porous wick, arterial wick, annular wick, and axial rectangular grooves are the wick configurations which HPAD has the capability of analyzing. For Vol. 1, see N74-22569.

  19. The Table of Specifications: A Tool for Instructional Design and Development.

    ERIC Educational Resources Information Center

    Dills, Charles R.

    1998-01-01

    Tables of specifications provide graphic representations of objectives and segments of instruction or test questions. Examines tables of specifications (use, significance of empty cells, traceability) and their application in instructional design, highlighting constructivism and microworlds, structural communication, computer mediated…

  20. Liquid Engine Design: Effect of Chamber Dimensions on Specific Impulse

    NASA Technical Reports Server (NTRS)

    Hoggard, Lindsay; Leahy, Joe

    2009-01-01

    Which assumption of combustion chemistry - frozen or equilibrium - should be used in the prediction of liquid rocket engine performance calculations? Can a correlation be developed for this? A literature search using the LaSSe tool, an online repository of old rocket data and reports, was completed. Test results of NTO/Aerozine-50 and Lox/LH2 subscale and full-scale injector and combustion chamber test results were found and studied for this task. NASA code, Chemical Equilibrium with Applications (CEA) was used to predict engine performance using both chemistry assumptions, defined here. Frozen- composition remains frozen during expansion through the nozzle. Equilibrium- instantaneous chemical equilibrium during nozzle expansion. Chamber parameters were varied to understand what dimensions drive chamber C* and Isp. Contraction Ratio is the ratio of the nozzle throat area to the area of the chamber. L is the length of the chamber. Characteristic chamber length, L*, is the length that the chamber would be if it were a straight tube and had no converging nozzle. Goal: Develop a qualitative and quantitative correlation for performance parameters - Specific Impulse (Isp) and Characteristic Velocity (C*) - as a function of one or more chamber dimensions - Contraction Ratio (CR), Chamber Length (L ) and/or Characteristic Chamber Length (L*). Determine if chamber dimensions can be correlated to frozen or equilibrium chemistry.

  1. Psychosocial Risks Generated By Assets Specific Design Software

    NASA Astrophysics Data System (ADS)

    Remus, Furtună; Angela, Domnariu; Petru, Lazăr

    2015-07-01

    The human activity concerning an occupation is resultant from the interaction between the psycho-biological, socio-cultural and organizational-occupational factors. Tehnological development, automation and computerization that are to be found in all the branches of activity, the level of speed in which things develop, as well as reaching their complexity, require less and less physical aptitudes and more cognitive qualifications. The person included in the work process is bound in most of the cases to come in line with the organizational-occupational situations that are specific to the demands of the job. The role of the programmer is essencial in the process of execution of ordered softwares, thus the truly brilliant ideas can only come from well-rested minds, concentrated on their tasks. The actual requirements of the jobs, besides the high number of benefits and opportunities, also create a series of psycho-social risks, which can increase the level of stress during work activity, especially for those who work under pressure.

  2. 24 CFR 3282.361 - Design Approval Primary Inspection Agency (DAPIA).

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... innovations which are not yet accepted practices. Acceptances by the Secretary shall be published in the form....) The DAPIA shall maintain a complete up-to-date set of approved designs and design changes approved... interested parties as needed when disputes arise. (5) Design change approval. The DAPIA shall also...

  3. 41 CFR 102-76.10 - What basic design and construction policy governs Federal agencies?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... basic design and construction policies: (a) Provide the highest quality services for designing and... Federal facilities that reflects the dignity, enterprise, vigor and stability of the Federal Government... requirements. (See 40 U.S.C. 3310 and 3312.) (d) Design Federal buildings to have a long life expectancy...

  4. Specification and preliminary design of an array processor

    NASA Technical Reports Server (NTRS)

    Slotnick, D. L.; Graham, M. L.

    1975-01-01

    The design of a computer suited to the class of problems typified by the general circulation of the atmosphere was investigated. A fundamental goal was that the resulting machine should have roughly 100 times the computing capability of an IBM 360/95 computer. A second requirement was that the machine should be programmable in a higher level language similar to FORTRAN. Moreover, the new machine would have to be compatible with the IBM 360/95 since the IBM machine would continue to be used for pre- and post-processing. A third constraint was that the cost of the new machine was to be significantly less than that of other extant machines of similar computing capability, such as the ILLIAC IV and CDC STAR. A final constraint was that it should be feasible to fabricate a complete system and put it in operation by early 1978. Although these objectives were generally met, considerable work remains to be done on the routing system.

  5. Overall plant design specification Modular High Temperature Gas-cooled Reactor. Revision 9

    SciTech Connect

    1990-05-01

    Revision 9 of the ``Overall Plant Design Specification Modular High Temperature Gas-Cooled Reactor,`` DOE-HTGR-86004 (OPDS) has been completed and is hereby distributed for use by the HTGR Program team members. This document, Revision 9 of the ``Overall Plant Design Specification`` (OPDS) reflects those changes in the MHTGR design requirements and configuration resulting form approved Design Change Proposals DCP BNI-003 and DCP BNI-004, involving the Nuclear Island Cooling and Spent Fuel Cooling Systems respectively.

  6. Earth Observatory Satellite system definition study. Report no. 5: System design and specifications. Part 1: Observatory system element specifications

    NASA Technical Reports Server (NTRS)

    1974-01-01

    The performance, design, and quality assurance requirements for the Earth Observatory Satellite (EOS) Observatory and Ground System program elements required to perform the Land Resources Management (LRM) A-type mission are presented. The requirements for the Observatory element with the exception of the instruments specifications are contained in the first part.

  7. Software design specification. Part 2: Orbital Flight Test (OFT) detailed design specification. Volume 3: Applications. Book 2: System management

    NASA Technical Reports Server (NTRS)

    1979-01-01

    The functions performed by the systems management (SM) application software are described along with the design employed to accomplish these functions. The operational sequences (OPS) control segments and the cyclic processes they control are defined. The SM specialist function control (SPEC) segments and the display controlled 'on-demand' processes that are invoked by either an OPS or SPEC control segment as a direct result of an item entry to a display are included. Each processing element in the SM application is described including an input/output table and a structured control flow diagram. The flow through the module and other information pertinent to that process and its interfaces to other processes are included.

  8. 41 CFR 102-74.340 - Who is responsible for monitoring and controlling areas designated for smoking by an agency head...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... with proper signage? 102-74.340 Section 102-74.340 Public Contracts and Property Management Federal... designated for smoking by an agency head and for identifying those areas with proper signage? Agency heads... smoking and identifying these areas with proper signage. Suitable, uniform signs reading...

  9. SP-Designer: a user-friendly program for designing species-specific primer pairs from DNA sequence alignments.

    PubMed

    Villard, Pierre; Malausa, Thibaut

    2013-07-01

    SP-Designer is an open-source program providing a user-friendly tool for the design of specific PCR primer pairs from a DNA sequence alignment containing sequences from various taxa. SP-Designer selects PCR primer pairs for the amplification of DNA from a target species on the basis of several criteria: (i) primer specificity, as assessed by interspecific sequence polymorphism in the annealing regions, (ii) the biochemical characteristics of the primers and (iii) the intended PCR conditions. SP-Designer generates tables, detailing the primer pair and PCR characteristics, and a FASTA file locating the primer sequences in the original sequence alignment. SP-Designer is Windows-compatible and freely available from http://www2.sophia.inra.fr/urih/sophia_mart/sp_designer/info_sp_designer.php.

  10. 24 CFR 3282.361 - Design Approval Primary Inspection Agency (DAPIA).

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ....362 who deal with the design, quality assurance manual, or manufactured homes built to them, and by... COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT MANUFACTURED HOME PROCEDURAL AND ENFORCEMENT... manufactured home designs submitted to it by the manufacturer and for assuring that they conform to...

  11. 24 CFR 3282.361 - Design Approval Primary Inspection Agency (DAPIA).

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ....362 who deal with the design, quality assurance manual, or manufactured homes built to them, and by... COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT MANUFACTURED HOME PROCEDURAL AND ENFORCEMENT... manufactured home designs submitted to it by the manufacturer and for assuring that they conform to...

  12. Design Thinking and the Deanly Conversation: Reflections on Conversation, Community, and Agency

    ERIC Educational Resources Information Center

    Campbell, Katy

    2015-01-01

    The article "Conversation as Inquiry: A Conversation with Instructional Designers" (Campbell, Schwier & Kenny 2006) appeared in the "Journal of Learning Design" Volume 1, Issue 3 in 2006. Nine years on, Professor Katy Campbell, Dean of the Faculty of Extension, University of Alberta, reflects upon the arguments articulated…

  13. GSP: A web-based platform for designing genome-specific primers in polyploids

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The sequences among subgenomes in a polyploid species have high similarity. This makes difficult to design genome-specific primers for sequence analysis. We present a web-based platform named GSP for designing genome-specific primers to distinguish subgenome sequences in the polyploid genome backgr...

  14. Preliminary design of the Carrisa Plains solar central receiver power plant. Volume II. Plant specifications

    SciTech Connect

    Price, R. E.

    1983-12-31

    The specifications and design criteria for all plant systems and subsystems used in developing the preliminary design of Carrisa Plains 30-MWe Solar Plant are contained in this volume. The specifications have been organized according to plant systems and levels. The levels are arranged in tiers. Starting at the top tier and proceeding down, the specification levels are the plant, system, subsystem, components, and fabrication. A tab number, listed in the index, has been assigned each document to facilitate document location.

  15. 41 CFR 102-76.10 - What basic design and construction policy governs Federal agencies?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... requirements. (See 40 U.S.C. 3310 and 3312.) (d) Design Federal buildings to have a long life expectancy and... accessible for maintenance, repair, or replacement without significantly disturbing occupied space....

  16. 41 CFR 102-76.10 - What basic design and construction policy governs Federal agencies?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... requirements. (See 40 U.S.C. 3310 and 3312.) (d) Design Federal buildings to have a long life expectancy and... accessible for maintenance, repair, or replacement without significantly disturbing occupied space....

  17. 49 CFR 107.402 - Application for designation as an approval or certification agency.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... knowing and willful concealment of a material fact, may subject the applicant to prosecution under the... designation must be in English and include the following information: (1) Name and address of the...

  18. Design and synthesis of a potent peptide containing both specific and non-specific cell-adhesion motifs.

    PubMed

    Lai, Yuxiao; Xie, Cao; Zhang, Zheng; Lu, Weiyue; Ding, Jiandong

    2010-06-01

    This article reports a potent chemical to promote cell adhesion on a substrate by combination of both moieties for specific and non-specific adhesion. The cyclic (-RGDfK-) (R: arginine, G: glycine, D: aspartic acid, f: D-phenylalanine, K: lysine) is employed to trigger specific cell adhesion, and a linear tripeptide KKK is introduced to enhance early non-specific cell adhesion. A series of cyclic and linear peptides with different charges were synthesized and then functionalized with thiol end-group. All the peptides were immobilized on gold layers, which were later passivated by bovine serum albumin. The coverage of NIH/3T3 fibroblast cells on the substrate modified by the linker containing both cyclic (-RGDfK-) and linear KKK is, surprisingly, significantly better than the summation using one of them, which reveals the strong cooperativity of specific and non-specific cell adhesions. The resultant cell adhesion on the substrates modified by appropriate linkers was much better than on tissue-culture plates. The cooperativity principle and the design strategy of the combined linker might be helpful for fundamental research of cell-material or cell-extracellular matrix interactions, and for modification of new biomaterials in regenerative medicine and targeted drug delivery.

  19. HAL/SM system functional design specification. [systems analysis and design analysis of central processing units

    NASA Technical Reports Server (NTRS)

    Ross, C.; Williams, G. P. W., Jr.

    1975-01-01

    The functional design of a preprocessor, and subsystems is described. A structure chart and a data flow diagram are included for each subsystem. Also a group of intermodule interface definitions (one definition per module) is included immediately following the structure chart and data flow for a particular subsystem. Each of these intermodule interface definitions consists of the identification of the module, the function the module is to perform, the identification and definition of parameter interfaces to the module, and any design notes associated with the module. Also described are compilers and computer libraries.

  20. Automatic generation of conceptual database design tools from data model specifications

    SciTech Connect

    Hong, Shuguang.

    1989-01-01

    The problems faced in the design and implementation of database software systems based on object-oriented data models are similar to that of other software design, i.e., difficult, complex, yet redundant effort. Automatic generation of database software system has been proposed as a solution to the problems. In order to generate database software system for a variety of object-oriented data models, two critical issues: data model specification and software generation, must be addressed. SeaWeed is a software system that automatically generates conceptual database design tools from data model specifications. A meta model has been defined for the specification of a class of object-oriented data models. This meta model provides a set of primitive modeling constructs that can be used to express the semantics, or unique characteristics, of specific data models. Software reusability has been adopted for the software generation. The technique of design reuse is utilized to derive the requirement specification of the software to be generated from data model specifications. The mechanism of code reuse is used to produce the necessary reusable software components. This dissertation presents the research results of SeaWeed including the meta model, data model specification, a formal representation of design reuse and code reuse, and the software generation paradigm.

  1. Tunnel support design by comparison of empirical and finite element analysis of the Nahakki tunnel in mohmand agency, pakistan

    NASA Astrophysics Data System (ADS)

    Riaz, Asif; Jamil, Syed Muhammad; Asif, Muhammad; Akhtar, Kamran

    2016-03-01

    The paper analyses the geological conditions of study area, rock mass strength parameters with suitable support structure propositions for the under construction Nahakki tunnel in Mohmand Agency. Geology of study area varies from mica schist to graphitic marble/phyllite to schist. The tunnel ground is classified and divided by the empisical classification systems like Rock mass rating (RMR), Q system (Q), and Geological strength index (GSI). Tunnel support measures are selected based on RMR and Q classification systems. Computer based finite element analysis (FEM) has given yet another dimension to design approach. FEM software Phase2 version 7.017 is used to calculate and compare deformations and stress concentrations around the tunnel, analyze interaction of support systems with excavated rock masses and verify and check the validity of empirically determined excavation and support systems.

  2. Collaborative design and use of an agency feedback form for student clinical practicum experience in community/public health nursing.

    PubMed

    Reilly, Janet Resop; Collier, Jill; Edelstein, Janice; Vandenhouten, Chris; Hovarter, Rebecca; Hansen, Judith M; Stewart, Stephanie; Turner, Mary Jo

    2012-01-01

    Evaluation of students in community and public health (C/PH) nursing clinical practica is a challenge, especially when preceptors are expected to evaluate students from different academic nursing programs. The need for a standardized student evaluation tool was identified during federally funded collaborative meetings held between C/PH academic and practice partners in Northeastern Wisconsin. This article focuses on the development and appraisal of the standardized Agency Feedback Form (AFF) for Student Practicum Experience in Community/Public Health Nursing, which was designed to meet the identified need. Four baccalaureate nursing programs implemented the AFF for 3 purposes: (1) to provide a consistent and easy evaluation form for preceptors to complete; (2) to communicate useful information about students' individual professional behaviors observed during practicum; and (3) to increase students' and preceptors' understanding of the population-based nursing interventions, using the Public Health Intervention Wheel. Future uses and implications of the AFF are also discussed.

  3. Earth Observatory Satellite system definition study. Report 5: System design and specifications. Volume 5: Specification for EROS operations control center

    NASA Technical Reports Server (NTRS)

    1974-01-01

    The functional, performance, and design requirements for the Operations Control Center (OCC) of the Earth Observatory Satellite (EOS) system are presented. The OCC controls the operations of the EOS satellite to acquire mission data consisting of: (1) thematic mapper data, (2) multispectral scanner data on EOS-A, or High Resolution Pointable Imager data on EOS-B, and (3) data collection system (DCS) data. The various inputs to the OCC are identified. The functional requirements of the OCC are defined. The specific systems and subsystems of the OCC are described and block diagrams are provided.

  4. Preliminary design specification for Department of Energy standardized spent nuclear fuel canisters. Volume 2: Rationale document

    SciTech Connect

    1998-08-19

    This document (Volume 2) is a companion document to a preliminary design specification for the design of canisters to be used during the handling, storage, transportation, and repository disposal of Department of Energy (DOE) spent nuclear fuel (SNF). This document contains no procurement information, such as the number of canisters to be fabricated, explicit timeframes for deliverables, etc. However, this rationale document does provide background information and design philosophy in order to help engineers better understand the established design criteria (contained in Volume 1 respectively) necessary to correctly design and fabricate these DOE SNF canisters.

  5. 12 CFR 1815.104 - Specific responsibilities of the designated Fund official.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Specific responsibilities of the designated... FUND, DEPARTMENT OF THE TREASURY ENVIRONMENTAL QUALITY § 1815.104 Specific responsibilities of the... decisionmaking processes to ensure that environmental factors are properly considered in all proposals...

  6. 36 CFR 907.5 - Specific responsibilities of designated Corporation official.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Specific responsibilities of... DEVELOPMENT CORPORATION ENVIRONMENTAL QUALITY § 907.5 Specific responsibilities of designated Corporation... Corporation's planning and decision-making processes to ensure that environmental factors are...

  7. Single Event Testing on Complex Devices: Test Like You Fly versus Test-Specific Design Structures

    NASA Technical Reports Server (NTRS)

    Berg, Melanie D.; Label, Kenneth A.

    2014-01-01

    We present a framework for evaluating complex digital systems targeted for harsh radiation environments such as space. Focus is limited to analyzing the single event upset (SEU) susceptibility of designs implemented inside Field Programmable Gate Array (FPGA) devices. Tradeoffs are provided between application-specific versus test-specific test structures.

  8. Single Event Testing on Complex Devices: Test Like You Fly Versus Test-Specific Design Structures

    NASA Technical Reports Server (NTRS)

    Berg, Melanie D.; Label, Kenneth

    2016-01-01

    We present a mechanism for evaluating complex digital systems targeted for harsh radiation environments such as space. Focus is limited to analyzing the single event upset (SEU) susceptibility of designs implemented inside Field Programmable Gate Array (FPGA) devices. Tradeoffs are provided between application-specific versus test-specific test structures.

  9. Better Educational Website Interface Design: The Implications from Gender-Specific Preferences in Graduate Students

    ERIC Educational Resources Information Center

    Hsu, Yu-chang

    2006-01-01

    This study investigated graduate students gender-specific preferences for certain website interface design features, intending to generate useful information for instructors in choosing and for website designers in creating educational websites. The features investigated in this study included colour value, major navigation buttons placement, and…

  10. Improving Students' Conceptual Understanding of a Specific Content Learning: A Designed Teaching Sequence

    ERIC Educational Resources Information Center

    Ahmad, N. J.; Lah, Y. Che

    2012-01-01

    The efficacy of a teaching sequence designed for a specific content of learning of electrochemistry is described in this paper. The design of the teaching draws upon theoretical insights into perspectives on learning and empirical studies to improve the teaching of this topic. A case study involving two classes, the experimental and baseline…

  11. A domain-specific design architecture for composite material design and aircraft part redesign

    NASA Technical Reports Server (NTRS)

    Punch, W. F., III; Keller, K. J.; Bond, W.; Sticklen, J.

    1992-01-01

    Advanced composites have been targeted as a 'leapfrog' technology that would provide a unique global competitive position for U.S. industry. Composites are unique in the requirements for an integrated approach to designing, manufacturing, and marketing of products developed utilizing the new materials of construction. Numerous studies extending across the entire economic spectrum of the United States from aerospace to military to durable goods have identified composites as a 'key' technology. In general there have been two approaches to composite construction: build models of a given composite materials, then determine characteristics of the material via numerical simulation and empirical testing; and experience-directed construction of fabrication plans for building composites with given properties. The first route sets a goal to capture basic understanding of a device (the composite) by use of a rigorous mathematical model; the second attempts to capture the expertise about the process of fabricating a composite (to date) at a surface level typically expressed in a rule based system. From an AI perspective, these two research lines are attacking distinctly different problems, and both tracks have current limitations. The mathematical modeling approach has yielded a wealth of data but a large number of simplifying assumptions are needed to make numerical simulation tractable. Likewise, although surface level expertise about how to build a particular composite may yield important results, recent trends in the KBS area are towards augmenting surface level problem solving with deeper level knowledge. Many of the relative advantages of composites, e.g., the strength:weight ratio, is most prominent when the entire component is designed as a unitary piece. The bottleneck in undertaking such unitary design lies in the difficulty of the re-design task. Designing the fabrication protocols for a complex-shaped, thick section composite are currently very difficult. It is in

  12. 23 CFR 636.107 - May contracting agencies use geographic preference in Federal-aid design-build or public-private...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Federal-aid design-build or public-private partnership projects? 636.107 Section 636.107 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS DESIGN-BUILD CONTRACTING General § 636.107 May contracting agencies use geographic preference in Federal-aid...

  13. 23 CFR 636.107 - May contracting agencies use geographic preference in Federal-aid design-build or public-private...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Federal-aid design-build or public-private partnership projects? 636.107 Section 636.107 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS DESIGN-BUILD CONTRACTING General § 636.107 May contracting agencies use geographic preference in Federal-aid...

  14. 23 CFR 636.107 - May contracting agencies use geographic preference in Federal-aid design-build or public-private...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Federal-aid design-build or public-private partnership projects? 636.107 Section 636.107 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS DESIGN-BUILD CONTRACTING General § 636.107 May contracting agencies use geographic preference in Federal-aid...

  15. 23 CFR 636.107 - May contracting agencies use geographic preference in Federal-aid design-build or public-private...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Federal-aid design-build or public-private partnership projects? 636.107 Section 636.107 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS DESIGN-BUILD CONTRACTING General § 636.107 May contracting agencies use geographic preference in Federal-aid...

  16. 23 CFR 636.107 - May contracting agencies use geographic preference in Federal-aid design-build or public-private...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Federal-aid design-build or public-private partnership projects? 636.107 Section 636.107 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS DESIGN-BUILD CONTRACTING General § 636.107 May contracting agencies use geographic preference in Federal-aid...

  17. [Ambivalence, old age and agency: Meaning of age-specific ambivalence for the construction of narrative identity].

    PubMed

    Richter, Anna Sarah

    2016-01-01

    Different models of the formation of age identity explain the empirically determined difference between the chronological age and the age which is subjectively perceived by elderly people themselves. From a biographical point of view and on the basis of two maximally contrasting empirical cases, this article investigates the ways ambivalence appears in the narrations of elderly people about personal aging and the narrative strategies of coping with this experience of ambivalence. The results of the analysis indicate that the conceptualization of age identity in the different models should be seen more as descriptions of potential but not necessary forms of coping with age-specific ambivalences.

  18. Laser communication experiment. Volume 1: Design study report: Spacecraft transceiver. Part 3: LCE design specifications

    NASA Technical Reports Server (NTRS)

    1970-01-01

    The requirements for the design, fabrication, performance, and testing of a 10.6 micron optical heterodyne receiver subsystem for use in a laser communication system are presented. The receiver subsystem, as a part of the laser communication experiment operates in the ATS 6 satellite and in a transportable ground station establishing two-way laser communications between the spacecraft and the transportable ground station. The conditions under which environmental tests are conducted are reported.

  19. Design of protein-interaction specificity affords selective bZIP-binding peptides

    PubMed Central

    Grigoryan, Gevorg; Reinke, Aaron W.; Keating, Amy E.

    2009-01-01

    Interaction specificity is a required feature of biological networks and a necessary characteristic of protein or small-molecule reagents and therapeutics. The ability to alter or inhibit protein interactions selectively would advance basic and applied molecular science. Assessing or modelling interaction specificity requires treating multiple competing complexes, which presents computational and experimental challenges. Here we present a computational framework for designing protein interaction specificity and use it to identify specific peptide partners for human bZIP transcription factors. Protein microarrays were used to characterize designed, synthetic ligands for all but one of 20 bZIP families. The bZIP proteins share strong sequence and structural similarities and thus are challenging targets to bind specifically. Yet many of the designs, including examples that bind the oncoproteins cJun, cFos and cMaf, were selective for their targets over all 19 other families. Collectively, the designs exhibit a wide range of novel interaction profiles, demonstrating that human bZIPs have only sparsely sampled the possible interaction space accessible to them. Our computational method provides a way to systematically analyze tradeoffs between stability and specificity and is suitable for use with many types of structure-scoring functions; thus it may prove broadly useful as a tool for protein design. PMID:19370028

  20. Designing specific protein–protein interactions using computation, experimental library screening, or integrated methods

    PubMed Central

    Chen, T Scott; Keating, Amy E

    2012-01-01

    Given the importance of protein–protein interactions for nearly all biological processes, the design of protein affinity reagents for use in research, diagnosis or therapy is an important endeavor. Engineered proteins would ideally have high specificities for their intended targets, but achieving interaction specificity by design can be challenging. There are two major approaches to protein design or redesign. Most commonly, proteins and peptides are engineered using experimental library screening and/or in vitro evolution. An alternative approach involves using protein structure and computational modeling to rationally choose sequences predicted to have desirable properties. Computational design has successfully produced novel proteins with enhanced stability, desired interactions and enzymatic function. Here we review the strengths and limitations of experimental library screening and computational structure-based design, giving examples where these methods have been applied to designing protein interaction specificity. We highlight recent studies that demonstrate strategies for combining computational modeling with library screening. The computational methods provide focused libraries predicted to be enriched in sequences with the properties of interest. Such integrated approaches represent a promising way to increase the efficiency of protein design and to engineer complex functionality such as interaction specificity. PMID:22593041

  1. 32 CFR 319.13 - Specific exemptions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... PROGRAM DEFENSE INTELLIGENCE AGENCY PRIVACY PROGRAM § 319.13 Specific exemptions. (a) All systems of records maintained by the Director Intelligence Agency shall be exempt from the requirements of 5 U.S.C... has been properly classified. (b) The Director, Defense Intelligence Agency, designated the systems...

  2. 32 CFR 319.13 - Specific exemptions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... PROGRAM DEFENSE INTELLIGENCE AGENCY PRIVACY PROGRAM § 319.13 Specific exemptions. (a) All systems of records maintained by the Director Intelligence Agency shall be exempt from the requirements of 5 U.S.C... has been properly classified. (b) The Director, Defense Intelligence Agency, designated the systems...

  3. Robust Design of Advanced Thermoelectric Conversion Systems: Probabilistic Design Impacts on Specific Power and Power Flux Optimization

    SciTech Connect

    Hendricks, Terry J.; Karri, Naveen K.

    2008-04-30

    Advanced, direct thermal energy conversion technologies are receiving increased research attention in order to recover waste thermal energy in advanced vehicles and industrial processes. Advanced thermoelectric (TE) systems necessarily require integrated system-level analyses to establish accurate optimum system designs. Past system-level design and analysis has relied on well-defined deterministic input parameters even though many critically important environmental and system design parameters in the above mentioned applications are often randomly variable, sometimes according to complex relationships, rather than discrete, well-known deterministic variables. This work describes new research and development creating techniques and capabilities for probabilistic design and analysis of advanced TE power generation systems to quantify the effects of randomly uncertain design inputs in determining more robust optimum TE system designs and expected outputs. Selected case studies involving stochastic TE .material properties demonstrate key stochastic material impacts on power, optimum TE area, specific power, and power flux in the TE design optimization process. Magnitudes and directions of these design modifications are quantified for selected TE system design analysis cases

  4. Design of LED-based reflector-array module for specific illuminance distribution

    NASA Astrophysics Data System (ADS)

    Chen, Enguo; Yu, Feihong

    2013-02-01

    This paper presents an efficient and practical design method for a LED based reflector-array lighting module. Improving on previous designs, this method could offer higher design freedom to achieve specific illuminance distribution for actual lighting application and deal with the LED light intensity distribution while shortening the design time. The detailed design description of the lighting system is thoroughly investigated. To demonstrate the effectiveness of this method, an ultra-compact reflector-array module, which produces a rectangular illumination area with a large aspect ratio, is specially designed to meet the high-demanding requirements of industrial lighting application. Design results show that most LED emitting energy could be collected into the required lighting region while higher-brightness and better-uniformity are simultaneously available within the focus region. It is expected that this method will have great potential for other lighting applications.

  5. From ergonomics to design specifications: contributions to the design of a processing machine in a tire company.

    PubMed

    Moraes, A S P; Arezes, P M; Vasconcelos, R

    2012-01-01

    The development of ergonomics' recommendations, guidelines and standards are attempts to promote the integration of ergonomics into industrial contexts. Such developments result from several sources and professionals and represent the effort that has been done to develop healthier and safer work environments. However, the availability of large amount of data and documents regarding ergonomics does not guarantee their applicability. The main goal of this paper is to use a specific case to demonstrate how ergonomics criteria were developed in order to contribute to the design of workplaces. Based on the obtained results from research undertaken in a tire company, it was observed that the ergonomics criteria should be presented as design specifications in order to be used by engineers and designers. In conclusion, it is observed that the multiple constraint environment impeded the appliance of the ergonomics criteria. It was also observed that the knowledge on technical design and the acquaintance with ergonomic standards, the level of integration in the design team, and the ability to communicate with workers and other technical staff have paramount importance in integrating ergonomics criteria into the design process.

  6. Attributes of effective and efficient kindergarten reading intervention: an examination of instructional time and design specificity.

    PubMed

    Simmons, Deborah C; Kame'enui, Edward J; Harn, Beth; Coyne, Michael D; Stoolmiller, Mike; Santoro, Lana Edwards; Smith, Sylvia B; Beck, Carrie Thomas; Kaufman, Noah K

    2007-01-01

    A randomized experimental design with three levels of intervention was used to compare the effects of beginning reading interventions on early phonemic, decoding, and spelling outcomes of 96 kindergartners identified as at risk for reading difficulty. The three instructional interventions varied systematically along two dimensions--time and design of instruction specificity--and consisted of (a) 30 min with high design specificity (30/H), (b) 15 min with high design specificity plus 15 min of non-code-based instruction (15/H+15), and (c) a commercial comparison condition that reflected 30 min of moderate design specificity instruction (30/M). With the exception of the second 15 min of the 15/H+15 condition, all instruction focused on phonemic, alphabetic, and orthographic skills and strategies. Students were randomly assigned to one of the three interventions and received 108 thirty-minute sessions of small-group instruction as a supplement to their typical half-day kindergarten experience. Planned comparisons indicated findings of statistical and practical significance that varied according to measure and students' entry-level performance. The results are discussed in terms of the pedagogical precision needed to design and provide effective and efficient instruction for students who are most at risk.

  7. Specification and Design of Electrical Flight System Architectures with SysML

    NASA Technical Reports Server (NTRS)

    McKelvin, Mark L., Jr.; Jimenez, Alejandro

    2012-01-01

    Modern space flight systems are required to perform more complex functions than previous generations to support space missions. This demand is driving the trend to deploy more electronics to realize system functionality. The traditional approach for the specification, design, and deployment of electrical system architectures in space flight systems includes the use of informal definitions and descriptions that are often embedded within loosely coupled but highly interdependent design documents. Traditional methods become inefficient to cope with increasing system complexity, evolving requirements, and the ability to meet project budget and time constraints. Thus, there is a need for more rigorous methods to capture the relevant information about the electrical system architecture as the design evolves. In this work, we propose a model-centric approach to support the specification and design of electrical flight system architectures using the System Modeling Language (SysML). In our approach, we develop a domain specific language for specifying electrical system architectures, and we propose a design flow for the specification and design of electrical interfaces. Our approach is applied to a practical flight system.

  8. Custom-Designed Molecular Scissors for Site-Specific Manipulation of the Plant and Mammalian Genomes

    NASA Astrophysics Data System (ADS)

    Kandavelou, Karthikeyan; Chandrasegaran, Srinivasan

    Zinc finger nucleases (ZFNs) are custom-designed molecular scissors, engineered to cut at specific DNA sequences. ZFNs combine the zinc finger proteins (ZFPs) with the nonspecific cleavage domain of the FokI restriction enzyme. The DNA-binding specificity of ZFNs can be easily altered experimentally. This easy manipulation of the ZFN recognition specificity enables one to deliver a targeted double-strand break (DSB) to a genome. The targeted DSB stimulates local gene targeting by several orders of magnitude at that specific cut site via homologous recombination (HR). Thus, ZFNs have become an important experimental tool to make site-specific and permanent alterations to genomes of not only plants and mammals but also of many other organisms. Engineering of custom ZFNs involves many steps. The first step is to identify a ZFN site at or near the chosen chromosomal target within the genome to which ZFNs will bind and cut. The second step is to design and/or select various ZFP combinations that will bind to the chosen target site with high specificity and affinity. The DNA coding sequence for the designed ZFPs are then assembled by polymerase chain reaction (PCR) using oligonucleotides. The third step is to fuse the ZFP constructs to the FokI cleavage domain. The ZFNs are then expressed as proteins by using the rabbit reticulocyte in vitro transcription/translation system and the protein products assayed for their DNA cleavage specificity.

  9. Custom-Designed Molecular Scissors for Site-Specific Manipulation of the Plant and Mammalian Genomes

    PubMed Central

    Kandavelou, Karthikeyan; Chandrasegaran, Srinivasan

    2010-01-01

    Summary Zinc finger nucleases (ZFNs) are custom-designed molecular scissors, engineered to cut at specific DNA sequences. ZFNs combine the zinc finger proteins (ZFPs) with the nonspecific cleavage domain of the FokI restriction enzyme. The DNA-binding specificity of ZFNs can be easily altered experimentally. This easy manipulation of the ZFN recognition specificity enables one to deliver a targeted double-strand break (DSB) to a genome. The targeted DSB stimulates local gene targeting by several orders of magnitude at that specific cut site via homologous recombination (HR). Thus, ZFNs have become an important experimental tool to make site-specific and permanent alterations to genomes of not only plants and mammals but also of many other organisms. Engineering of custom ZFNs involves many steps. The first step is to identify a ZFN site at or near the chosen chromosomal target within the genome to which ZFNs will bind and cut. The second step is to design and/or select various ZFP combinations that will bind to the chosen target site with high specificity and affinity. The DNA coding sequence for the designed ZFPs are then assembled by polymerase chain reaction (PCR) using oligonucleotides. The third step is to fuse the ZFP constructs to the FokI cleavage domain. The ZFNs are then expressed as proteins by using the rabbit reticulocyte in vitro transcription/translation system and the protein products assayed for their DNA cleavage specificity. PMID:19488728

  10. Structural, kinetic, and thermodynamic studies of specificity designed HIV-1 protease

    SciTech Connect

    Alvizo, Oscar; Mittal, Seema; Mayo, Stephen L.; Schiffer, Celia A.

    2012-10-23

    HIV-1 protease recognizes and cleaves more than 12 different substrates leading to viral maturation. While these substrates share no conserved motif, they are specifically selected for and cleaved by protease during viral life cycle. Drug resistant mutations evolve within the protease that compromise inhibitor binding but allow the continued recognition of all these substrates. While the substrate envelope defines a general shape for substrate recognition, successfully predicting the determinants of substrate binding specificity would provide additional insights into the mechanism of altered molecular recognition in resistant proteases. We designed a variant of HIV protease with altered specificity using positive computational design methods and validated the design using X-ray crystallography and enzyme biochemistry. The engineered variant, Pr3 (A28S/D30F/G48R), was designed to preferentially bind to one out of three of HIV protease's natural substrates; RT-RH over p2-NC and CA-p2. In kinetic assays, RT-RH binding specificity for Pr3 increased threefold compared to the wild-type (WT), which was further confirmed by isothermal titration calorimetry. Crystal structures of WT protease and the designed variant in complex with RT-RH, CA-p2, and p2-NC were determined. Structural analysis of the designed complexes revealed that one of the engineered substitutions (G48R) potentially stabilized heterogeneous flap conformations, thereby facilitating alternate modes of substrate binding. Our results demonstrate that while substrate specificity could be engineered in HIV protease, the structural pliability of protease restricted the propagation of interactions as predicted. These results offer new insights into the plasticity and structural determinants of substrate binding specificity of the HIV-1 protease.

  11. Structural, kinetic, and thermodynamic studies of specificity designed HIV-1 protease.

    PubMed

    Alvizo, Oscar; Mittal, Seema; Mayo, Stephen L; Schiffer, Celia A

    2012-07-01

    HIV-1 protease recognizes and cleaves more than 12 different substrates leading to viral maturation. While these substrates share no conserved motif, they are specifically selected for and cleaved by protease during viral life cycle. Drug resistant mutations evolve within the protease that compromise inhibitor binding but allow the continued recognition of all these substrates. While the substrate envelope defines a general shape for substrate recognition, successfully predicting the determinants of substrate binding specificity would provide additional insights into the mechanism of altered molecular recognition in resistant proteases. We designed a variant of HIV protease with altered specificity using positive computational design methods and validated the design using X-ray crystallography and enzyme biochemistry. The engineered variant, Pr3 (A28S/D30F/G48R), was designed to preferentially bind to one out of three of HIV protease's natural substrates; RT-RH over p2-NC and CA-p2. In kinetic assays, RT-RH binding specificity for Pr3 increased threefold compared to the wild-type (WT), which was further confirmed by isothermal titration calorimetry. Crystal structures of WT protease and the designed variant in complex with RT-RH, CA-p2, and p2-NC were determined. Structural analysis of the designed complexes revealed that one of the engineered substitutions (G48R) potentially stabilized heterogeneous flap conformations, thereby facilitating alternate modes of substrate binding. Our results demonstrate that while substrate specificity could be engineered in HIV protease, the structural pliability of protease restricted the propagation of interactions as predicted. These results offer new insights into the plasticity and structural determinants of substrate binding specificity of the HIV-1 protease.

  12. Computational design of a red fluorophore ligase for site-specific protein labeling in living cells

    DOE PAGES

    Liu, Daniel S.; Nivon, Lucas G.; Richter, Florian; Goldman, Peter J.; Deerinck, Thomas J.; Yao, Jennifer Z.; Richardson, Douglas; Phipps, William S.; Ye, Anne Z.; Ellisman, Mark H.; et al

    2014-10-13

    In this study, chemical fluorophores offer tremendous size and photophysical advantages over fluorescent proteins but are much more challenging to target to specific cellular proteins. Here, we used Rosetta-based computation to design a fluorophore ligase that accepts the red dye resorufin, starting from Escherichia coli lipoic acid ligase. X-ray crystallography showed that the design closely matched the experimental structure. Resorufin ligase catalyzed the site-specific and covalent attachment of resorufin to various cellular proteins genetically fused to a 13-aa recognition peptide in multiple mammalian cell lines and in primary cultured neurons. We used resorufin ligase to perform superresolution imaging of themore » intermediate filament protein vimentin by stimulated emission depletion and electron microscopies. This work illustrates the power of Rosetta for major redesign of enzyme specificity and introduces a tool for minimally invasive, highly specific imaging of cellular proteins by both conventional and superresolution microscopies.« less

  13. Computational design of a red fluorophore ligase for site-specific protein labeling in living cells

    SciTech Connect

    Liu, Daniel S.; Nivon, Lucas G.; Richter, Florian; Goldman, Peter J.; Deerinck, Thomas J.; Yao, Jennifer Z.; Richardson, Douglas; Phipps, William S.; Ye, Anne Z.; Ellisman, Mark H.; Drennan, Catherine L.; Baker, David; Ting, Alice Y.

    2014-10-13

    In this study, chemical fluorophores offer tremendous size and photophysical advantages over fluorescent proteins but are much more challenging to target to specific cellular proteins. Here, we used Rosetta-based computation to design a fluorophore ligase that accepts the red dye resorufin, starting from Escherichia coli lipoic acid ligase. X-ray crystallography showed that the design closely matched the experimental structure. Resorufin ligase catalyzed the site-specific and covalent attachment of resorufin to various cellular proteins genetically fused to a 13-aa recognition peptide in multiple mammalian cell lines and in primary cultured neurons. We used resorufin ligase to perform superresolution imaging of the intermediate filament protein vimentin by stimulated emission depletion and electron microscopies. This work illustrates the power of Rosetta for major redesign of enzyme specificity and introduces a tool for minimally invasive, highly specific imaging of cellular proteins by both conventional and superresolution microscopies.

  14. Computational design of a red fluorophore ligase for site-specific protein labeling in living cells

    PubMed Central

    Liu, Daniel S.; Nivón, Lucas G.; Richter, Florian; Goldman, Peter J.; Deerinck, Thomas J.; Yao, Jennifer Z.; Richardson, Douglas; Phipps, William S.; Ye, Anne Z.; Ellisman, Mark H.; Drennan, Catherine L.; Baker, David; Ting, Alice Y.

    2014-01-01

    Chemical fluorophores offer tremendous size and photophysical advantages over fluorescent proteins but are much more challenging to target to specific cellular proteins. Here, we used Rosetta-based computation to design a fluorophore ligase that accepts the red dye resorufin, starting from Escherichia coli lipoic acid ligase. X-ray crystallography showed that the design closely matched the experimental structure. Resorufin ligase catalyzed the site-specific and covalent attachment of resorufin to various cellular proteins genetically fused to a 13-aa recognition peptide in multiple mammalian cell lines and in primary cultured neurons. We used resorufin ligase to perform superresolution imaging of the intermediate filament protein vimentin by stimulated emission depletion and electron microscopies. This work illustrates the power of Rosetta for major redesign of enzyme specificity and introduces a tool for minimally invasive, highly specific imaging of cellular proteins by both conventional and superresolution microscopies. PMID:25313043

  15. Using Space Weather Variability in Evaluating the Radiation Environment Design Specifications for NASA's Constellation Program

    NASA Technical Reports Server (NTRS)

    Coffey, Victoria N.; Blackwell, William C.; Minow, Joseph I.; Bruce, Margaret B.; Howard, James W.

    2007-01-01

    NASA's Constellation program, initiated to fulfill the Vision for Space Exploration, will create a new generation of vehicles for servicing low Earth orbit, the Moon, and beyond. Space radiation specifications for space system hardware are necessarily conservative to assure system robustness for a wide range of space environments. Spectral models of solar particle events and trapped radiation belt environments are used to develop the design requirements for estimating total ionizing radiation dose, displacement damage, and single event effects for Constellation hardware. We first describe the rationale using the spectra chosen to establish the total dose and single event design environmental specifications for Constellation systems. We then compare variability of the space environment to the spectral design models to evaluate their applicability as conservative design environments and potential vulnerabilities to extreme space weather events

  16. Method for Predicting the Energy Characteristics of Li-Ion Cells Designed for High Specific Energy

    NASA Technical Reports Server (NTRS)

    Bennett, William, R.

    2012-01-01

    Novel electrode materials with increased specific capacity and voltage performance are critical to the NASA goals for developing Li-ion batteries with increased specific energy and energy density. Although performance metrics of the individual electrodes are critically important, a fundamental understanding of the interactions of electrodes in a full cell is essential to achieving the desired performance, and for establishing meaningful goals for electrode performance in the first place. This paper presents design considerations for matching positive and negative electrodes in a viable design. Methods for predicting cell-level performance, based on laboratory data for individual electrodes, are presented and discussed.

  17. Designing patient-specific 3D printed craniofacial implants using a novel topology optimization method.

    PubMed

    Sutradhar, Alok; Park, Jaejong; Carrau, Diana; Nguyen, Tam H; Miller, Michael J; Paulino, Glaucio H

    2016-07-01

    Large craniofacial defects require efficient bone replacements which should not only provide good aesthetics but also possess stable structural function. The proposed work uses a novel multiresolution topology optimization method to achieve the task. Using a compliance minimization objective, patient-specific bone replacement shapes can be designed for different clinical cases that ensure revival of efficient load transfer mechanisms in the mid-face. In this work, four clinical cases are introduced and their respective patient-specific designs are obtained using the proposed method. The optimized designs are then virtually inserted into the defect to visually inspect the viability of the design . Further, once the design is verified by the reconstructive surgeon, prototypes are fabricated using a 3D printer for validation. The robustness of the designs are mechanically tested by subjecting them to a physiological loading condition which mimics the masticatory activity. The full-field strain result through 3D image correlation and the finite element analysis implies that the solution can survive the maximum mastication of 120 lb. Also, the designs have the potential to restore the buttress system and provide the structural integrity. Using the topology optimization framework in designing the bone replacement shapes would deliver surgeons new alternatives for rather complicated mid-face reconstruction. PMID:26660897

  18. Designing patient-specific 3D printed craniofacial implants using a novel topology optimization method.

    PubMed

    Sutradhar, Alok; Park, Jaejong; Carrau, Diana; Nguyen, Tam H; Miller, Michael J; Paulino, Glaucio H

    2016-07-01

    Large craniofacial defects require efficient bone replacements which should not only provide good aesthetics but also possess stable structural function. The proposed work uses a novel multiresolution topology optimization method to achieve the task. Using a compliance minimization objective, patient-specific bone replacement shapes can be designed for different clinical cases that ensure revival of efficient load transfer mechanisms in the mid-face. In this work, four clinical cases are introduced and their respective patient-specific designs are obtained using the proposed method. The optimized designs are then virtually inserted into the defect to visually inspect the viability of the design . Further, once the design is verified by the reconstructive surgeon, prototypes are fabricated using a 3D printer for validation. The robustness of the designs are mechanically tested by subjecting them to a physiological loading condition which mimics the masticatory activity. The full-field strain result through 3D image correlation and the finite element analysis implies that the solution can survive the maximum mastication of 120 lb. Also, the designs have the potential to restore the buttress system and provide the structural integrity. Using the topology optimization framework in designing the bone replacement shapes would deliver surgeons new alternatives for rather complicated mid-face reconstruction.

  19. Mod-5A wind turbine generator program design report. Volume 4: Drawings and specifications, book 3

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The design, development and analysis of the 7.3 MW MOD-5A wind turbine generator is documented. This volume contains the drawings and specifications developed for the final design. This volume is divided into 5 books of which this is the third, containing drawings 47A380074 through 47A380126. A full breakdown parts listing is provided as well as a where used list.

  20. Design of Optimal Treatments for Neuromusculoskeletal Disorders using Patient-Specific Multibody Dynamic Models

    PubMed Central

    Fregly, Benjamin J.

    2011-01-01

    Disorders of the human neuromusculoskeletal system such as osteoarthritis, stroke, cerebral palsy, and paraplegia significantly affect mobility and result in a decreased quality of life. Surgical and rehabilitation treatment planning for these disorders is based primarily on static anatomic measurements and dynamic functional measurements filtered through clinical experience. While this subjective treatment planning approach works well in many cases, it does not predict accurate functional outcome in many others. This paper presents a vision for how patient-specific multibody dynamic models can serve as the foundation for an objective treatment planning approach that identifies optimal treatments and treatment parameters on an individual patient basis. First, a computational paradigm is presented for constructing patient-specific multibody dynamic models. This paradigm involves a combination of patient-specific skeletal models, muscle-tendon models, neural control models, and articular contact models, with the complexity of the complete model being dictated by the requirements of the clinical problem being addressed. Next, three clinical applications are presented to illustrate how such models could be used in the treatment design process. One application involves the design of patient-specific gait modification strategies for knee osteoarthritis rehabilitation, a second involves the selection of optimal patient-specific surgical parameters for a particular knee osteoarthritis surgery, and the third involves the design of patient-specific muscle stimulation patterns for stroke rehabilitation. The paper concludes by discussing important challenges that need to be overcome to turn this vision into reality. PMID:21785529

  1. Mod-5A Wind Turbine Generator Program Design Report. Volume 4: Drawings and Specifications, Book 1

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The design, development and analysis of the 7.3 MW MOD-5A wind turbine generator is documented. Volume 4 contains the drawings and specifications that were developed in preparation for building the MOD-5A wind turbine generator. This is the first of five books of volume four. It contains structural design criteria, generator step-up transformer specs, specs for design, fabrication and testing of the system, specs for the ground control enclosure, systems specs, slip ring specs, and control system specs.

  2. Towards the formal specification of the requirements and design of a processor interface unit: HOL listings

    NASA Technical Reports Server (NTRS)

    Fura, David A.; Windley, Phillip J.; Cohen, Gerald C.

    1993-01-01

    This technical report contains the HOL listings of the specification of the design and major portions of the requirements for a commercially developed processor interface unit (or PIU). The PIU is an interface chip performing memory interface, bus interface, and additional support services for a commercial microprocessor within a fault-tolerant computer system. This system, the Fault-Tolerant Embedded Processor (FTEP), is targeted towards applications in avionics and space requiring extremely high levels of mission reliability, extended maintenance-free operation, or both. This report contains the actual HOL listings of the PIU specification as it currently exists. Section two of this report contains general-purpose HOL theories that support the PIU specification. These theories include definitions for the hardware components used in the PIU, our implementation of bit words, and our implementation of temporal logic. Section three contains the HOL listings for the PIU design specification. Aside from the PIU internal bus (I-Bus), this specification is complete. Section four contains the HOL listings for a major portion of the PIU requirements specification. Specifically, it contains most of the definition for the PIU behavior associated with memory accesses initiated by the local processor.

  3. Just-in-time Design and Additive Manufacture of Patient-specific Medical Implants

    NASA Astrophysics Data System (ADS)

    Shidid, Darpan; Leary, Martin; Choong, Peter; Brandt, Milan

    Recent advances in medical imaging and manufacturing science have enabled the design and production of complex, patient-specific orthopaedic implants. Additive Manufacture (AM) generates three-dimensional structures layer by layer, and is not subject to the constraints associated with traditional manufacturing methods. AM provides significant opportunities for the design of novel geometries and complex lattice structures with enhanced functional performance. However, the design and manufacture of patient-specific AM implant structures requires unique expertise in handling various optimization platforms. Furthermore, the design process for complex structures is computationally intensive. The primary aim of this research is to enable the just-in-time customisation of AM prosthesis; whereby AM implant design and manufacture be completed within the time constraints of a single surgical procedure, while minimising prosthesis mass and optimising the lattice structure to match the stiffness of the surrounding bone tissue. In this research, a design approach using raw CT scan data is applied to the AM manufacture of femoral prosthesis. Using the proposed just-in-time concept, the mass of the prosthesis was rapidly designed and manufactured while satisfying the associated structural requirements. Compressive testing of lattice structures manufactured using proposed method shows that the load carrying capacity of the resected composite bone can be recovered by up to 85% and the compressive stiffness of the AM prosthesis is statistically indistinguishable from the stiffness of the initial bone.

  4. Spaceborne computer executive routine functional design specification. Volume 2: Computer executive design for space station/base

    NASA Technical Reports Server (NTRS)

    Kennedy, J. R.; Fitzpatrick, W. S.

    1971-01-01

    The computer executive functional system design concepts derived from study of the Space Station/Base are presented. Information Management System hardware configuration as directly influencing the executive design is reviewed. The hardware configuration and generic executive design requirements are considered in detail in a previous report (System Configuration and Executive Requirements Specifications for Reusable Shuttle and Space Station/Base, 9/25/70). This report defines basic system primitives and delineates processes and process control. Supervisor states are considered for describing basic multiprogramming and multiprocessing systems. A high-level computer executive including control of scheduling, allocation of resources, system interactions, and real-time supervisory functions is defined. The description is oriented to provide a baseline for a functional simulation of the computer executive system.

  5. Assessing cost-effectiveness of specific LID practice designs in response to large storm events

    NASA Astrophysics Data System (ADS)

    Chui, Ting Fong May; Liu, Xin; Zhan, Wenting

    2016-02-01

    Low impact development (LID) practices have become more important in urban stormwater management worldwide. However, most research on design optimization focuses on relatively large scale, and there is very limited information or guideline regarding individual LID practice designs (i.e., optimal depth, width and length). The objective of this study is to identify the optimal design by assessing the hydrological performance and the cost-effectiveness of different designs of LID practices at a household or business scale, and to analyze the sensitivity of the hydrological performance and the cost of the optimal design to different model and design parameters. First, EPA SWMM, automatically controlled by MATLAB, is used to obtain the peak runoff of different designs of three specific LID practices (i.e., green roof, bioretention and porous pavement) under different design storms (i.e., 2 yr and 50 yr design storms of Hong Kong, China and Seattle, U.S.). Then, life cycle cost is estimated for the different designs, and the optimal design, defined as the design with the lowest cost and at least 20% peak runoff reduction, is identified. Finally, sensitivity of the optimal design to the different design parameters is examined. The optimal design of green roof tends to be larger in area but thinner, while the optimal designs of bioretention and porous pavement tend to be smaller in area. To handle larger storms, however, it is more effective to increase the green roof depth, and to increase the area of the bioretention and porous pavement. Porous pavement is the most cost-effective for peak flow reduction, followed by bioretention and then green roof. The cost-effectiveness, measured as the peak runoff reduction/thousand Dollars of LID practices in Hong Kong (e.g., 0.02 L/103 US s, 0.15 L/103 US s and 0.93 L/103 US s for green roof, bioretention and porous pavement for 2 yr storm) is lower than that in Seattle (e.g., 0.03 L/103 US s, 0.29 L/103 US s and 1.58 L/103 US s for

  6. 40 CFR 53.51 - Demonstration of compliance with design specifications and manufacturing and test requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... finish of surfaces specified to be anodized—meet the specifications of 40 CFR part 50, appendix L or... in 40 CFR part 50, appendix L. (c) Sampler manufacturing quality control. The manufacturer must... a FRM or FEM and that are specified by design in 40 CFR part 50, appendix L or O (as...

  7. 40 CFR 53.51 - Demonstration of compliance with design specifications and manufacturing and test requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... finish of surfaces specified to be anodized—meet the specifications of 40 CFR part 50, appendix L or... in 40 CFR part 50, appendix L. (c) Sampler manufacturing quality control. The manufacturer must... FRM or FEM and that are specified by design in 40 CFR part 50, appendix L or O (as applicable),...

  8. System design and specifications. Earth Observatory Satellite system definition study (EOS)

    NASA Technical Reports Server (NTRS)

    1974-01-01

    A design summary of the Earth Observatory Satellite (EOS) is presented. The systems considered in the summary are: (1) the spacecraft structure, (2) electrical power modules, (3) communications and data handling module, (4) attitude determination module, (5) actuation module, and (6) solar array and drive module. The documents which provide the specifications for the systems and the equipment are identified.

  9. GSP: a web-based platform for designing genome-specific primers in polyploids

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The primary goal of this research was to develop a web-based platform named GSP for designing genome-specific primers to distinguish subgenome sequences in the polyploid genome background. GSP uses BLAST to extract homeologous sequences of the subgenomes in the existing databases, performed a multip...

  10. 40 CFR 53.51 - Demonstration of compliance with design specifications and manufacturing and test requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... finish of surfaces specified to be anodized—meet the specifications of 40 CFR part 50, appendix L or... in 40 CFR part 50, appendix L. (c) Sampler manufacturing quality control. The manufacturer must... a FRM or FEM and that are specified by design in 40 CFR part 50, appendix L or O (as...

  11. 40 CFR 53.51 - Demonstration of compliance with design specifications and manufacturing and test requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... finish of surfaces specified to be anodized—meet the specifications of 40 CFR part 50, appendix L or... in 40 CFR part 50, appendix L. (c) Sampler manufacturing quality control. The manufacturer must... FRM or FEM and that are specified by design in 40 CFR part 50, appendix L or O (as applicable),...

  12. 40 CFR 53.51 - Demonstration of compliance with design specifications and manufacturing and test requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... finish of surfaces specified to be anodized—meet the specifications of 40 CFR part 50, appendix L or... in 40 CFR part 50, appendix L. (c) Sampler manufacturing quality control. The manufacturer must... FRM or FEM and that are specified by design in 40 CFR part 50, appendix L or O (as applicable),...

  13. Project Prevention - A Curriculum Specifically Designed To Prevent Illegal Drug Use among Students with Disabilties: Secondary.

    ERIC Educational Resources Information Center

    Devlin, Sandy; And Others

    The Project Prevention curriculum is specifically designed for the prevention of substance abuse among students with disabilities and was piloted in 10 classrooms serving students with mild, moderate, and severe disabilities. This component of the curriculum is intended for secondary students and includes four sections with a total of 67 lesson…

  14. Design, synthesis, and characterization of nucleosomes containing site-specific DNA damage.

    PubMed

    Taylor, John-Stephen

    2015-12-01

    How DNA damaged is formed, recognized, and repaired in chromatin is an area of intense study. To better understand the structure activity relationships of damaged chromatin, mono and dinucleosomes containing site-specific damage have been prepared and studied. This review will focus on the design, synthesis, and characterization of model systems of damaged chromatin for structural, physical, and enzymatic studies.

  15. Functional design specification for Stowage List And Hardware Tracking System (SLAHTS). [space shuttles

    NASA Technical Reports Server (NTRS)

    Keltner, D. J.

    1975-01-01

    This functional design specification defines the total systems approach to meeting the requirements stated in the Detailed Requirements Document for Stowage List and Hardware Tracking System for the space shuttle program. The stowage list and hardware tracking system is identified at the system and subsystem level with each subsystem defined as a function of the total system.

  16. Systems design study of the Pioneer Venus spacecraft. Volume 3. Specifications

    NASA Technical Reports Server (NTRS)

    1973-01-01

    Pioneer Venus spacecraft performance requirements are presented. The specifications include: (1) Design criteria and performance requirements for the Pioneer Venus spacecraft systems and subsystems for a 1978 multiprobe mission and a 1978 orbiter mission, spacecraft system interface, and scientific instrument integration.

  17. Investigating an Open Methodology for Designing Domain-Specific Language Collections

    ERIC Educational Resources Information Center

    Fitzgerald, Alannah; Wu, Shaoqun; Barge, Martin

    2014-01-01

    With this research and design paper, we are proposing that Open Educational Resources (OERs) and Open Access (OA) publications give increasing access to high quality online educational and research content for the development of powerful domain-specific language collections that can be further enhanced linguistically with the Flexible Language…

  18. Designing Peace and Conflict Exercises: Level of Analysis, Scenario, and Role Specification

    ERIC Educational Resources Information Center

    Bartels, Elizabeth; McCown, Margaret; Wilkie, Timothy

    2013-01-01

    Attentiveness to and transparency about the methodological implications of the level of analysis selected for peace and conflict exercises constitute essential elements of good game design. The article explores the impact of level of analysis choices in the context of two key portions of exercises, scenario construction and role specification. It…

  19. Claims, Evidence, and Achievement-Level Descriptors as a Foundation for Item Design and Test Specifications

    ERIC Educational Resources Information Center

    Hendrickson, Amy; Huff, Kristen; Luecht, Richard

    2010-01-01

    Evidence-centered assessment design (ECD) explicates a transparent evidentiary argument to warrant the inferences we make from student test performance. This article describes how the vehicles for gathering student evidence--task models and test specifications--are developed. Task models, which are the basis for item development, flow directly…

  20. A bibliography on formal methods for system specification, design and validation

    NASA Technical Reports Server (NTRS)

    Meyer, J. F.; Furchtgott, D. G.; Movaghar, A.

    1982-01-01

    Literature on the specification, design, verification, testing, and evaluation of avionics systems was surveyed, providing 655 citations. Journal papers, conference papers, and technical reports are included. Manual and computer-based methods were employed. Keywords used in the online search are listed.

  1. Mod-5A wind turbine generator program design report. Volume 4: Drawings and specifications, book 5

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The design, development and analysis of the 7.3 MW MOD-5A wind turbine generator is documented. There are four volumes. This volume contains the drawings and specifications that were developed in preparation for building the MOD-5A wind turbine generator. Detail drawings of several assemblies and subassemblies are given. This is the fifth book of volume 4.

  2. 7 CFR 1717.605 - Design standards, plans and specifications, construction standards, and RUS accepted materials.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., construction standards, and RUS accepted materials. 1717.605 Section 1717.605 Agriculture Regulations of the... standards, plans and specifications, construction standards, and RUS accepted materials. All borrowers... system design, construction standards, and the use of RUS accepted materials. Borrowers must comply...

  3. Visual design and verification tool for collision-free dexterous patient specific neurosurgical instruments

    NASA Astrophysics Data System (ADS)

    Hess, Maggie; Eastwood, Kyle; Linder, Bence; Bodani, Vivek; Lasso, Andras; Looi, Thomas; Fichtinger, Gabor; Drake, James

    2016-03-01

    PURPOSE: In many minimally invasive neurosurgical procedures, the surgical workspace is a small tortuous cavity that is accessed using straight, rigid instruments with limited dexterity. Specifically considering neuroendoscopy, it is often challenging for surgeons, using standard instruments, to reach multiple surgical targets from a single incision. To address this problem, continuum tools are under development to create highly dexterous minimally invasive instruments. However, this design process is not trivial, and therefore, a user-friendly design platform capable of easily incorporating surgeon input is needed. METHODS: We propose a method that uses simulation and visual verification to design continuum tools that are patient and procedure specific. Our software module utilizes pre-operative scans and virtual threedimensional (3D) patient models to intuitively aid instrument design. The user specifies basic tool parameters and the parameterized tools and trocar are modeled within the virtual patient. By selecting and dragging the instrument models, the tools are instantly reshaped and repositioned. The tool geometry and surgical entry points are then returned as outputs to undergo optimization. We have completed an initial validation of the software by comparing a simulation of a physical instrument's reachability to the corresponding virtual design. RESULTS AND CONCLUSION: The software was assessed qualitatively by two neurosurgeons, who design tools for an intraventricular endoscopic procedure. Further, validation experiments comparing the design of a virtual instrument to a physical tool demonstrate that the software module functions correctly. Thus, our platform permits user-friendly, application specific design of continuum instruments. These instruments will give surgeons much more flexibility in developing future minimally invasive procedures.

  4. Rational design of antibodies targeting specific epitopes within intrinsically disordered proteins

    PubMed Central

    Sormanni, Pietro; Aprile, Francesco A.; Vendruscolo, Michele

    2015-01-01

    Antibodies are powerful tools in life sciences research, as well as in diagnostic and therapeutic applications, because of their ability to bind given molecules with high affinity and specificity. Using current methods, however, it is laborious and sometimes difficult to generate antibodies to target specific epitopes within a protein, in particular if these epitopes are not effective antigens. Here we present a method to rationally design antibodies to enable them to bind virtually any chosen disordered epitope in a protein. The procedure consists in the sequence-based design of one or more complementary peptides targeting a selected disordered epitope and the subsequent grafting of such peptides on an antibody scaffold. We illustrate the method by designing six single-domain antibodies to bind different epitopes within three disease-related intrinsically disordered proteins and peptides (α-synuclein, Aβ42, and IAPP). Our results show that all these designed antibodies bind their targets with good affinity and specificity. As an example of an application, we show that one of these antibodies inhibits the aggregation of α-synuclein at substoichiometric concentrations and that binding occurs at the selected epitope. Taken together, these results indicate that the design strategy that we propose makes it possible to obtain antibodies targeting given epitopes in disordered proteins or protein regions. PMID:26216991

  5. Rational design of antibodies targeting specific epitopes within intrinsically disordered proteins.

    PubMed

    Sormanni, Pietro; Aprile, Francesco A; Vendruscolo, Michele

    2015-08-11

    Antibodies are powerful tools in life sciences research, as well as in diagnostic and therapeutic applications, because of their ability to bind given molecules with high affinity and specificity. Using current methods, however, it is laborious and sometimes difficult to generate antibodies to target specific epitopes within a protein, in particular if these epitopes are not effective antigens. Here we present a method to rationally design antibodies to enable them to bind virtually any chosen disordered epitope in a protein. The procedure consists in the sequence-based design of one or more complementary peptides targeting a selected disordered epitope and the subsequent grafting of such peptides on an antibody scaffold. We illustrate the method by designing six single-domain antibodies to bind different epitopes within three disease-related intrinsically disordered proteins and peptides (α-synuclein, Aβ42, and IAPP). Our results show that all these designed antibodies bind their targets with good affinity and specificity. As an example of an application, we show that one of these antibodies inhibits the aggregation of α-synuclein at substoichiometric concentrations and that binding occurs at the selected epitope. Taken together, these results indicate that the design strategy that we propose makes it possible to obtain antibodies targeting given epitopes in disordered proteins or protein regions.

  6. Specificity of Methylation Assays in Cancer Research: A Guideline for Designing Primers and Probes

    PubMed Central

    Barekati, Zeinab; Radpour, Ramin; Kohler, Corina; Zhong, Xiao Yan

    2010-01-01

    DNA methylation is an epigenetic regulation mechanism of genomic function, and aberrant methylation pattern has been found to be a common event in many diseases and human cancers. A large number of cancer studies have been focused on identification of methylation changes as biomarkers (i.e., breast cancer). However, still clinical use of them is very limited because of lack of specificity and sensitivity for diagnostic test. This highlights the critical need for specific primer and probe design to avoid false-positive detection of methylation profiling. The guideline and online web tools that are introduced in this paper might help to perform a successful experiment and to develop specific diagnosis biomarkers by designing right primer pair and probe prior to experimental step. PMID:20798774

  7. Testing Requirements to Manage Data Exchange Specifications in Enterprise Integration - A Schema Design Quality Focus.

    SciTech Connect

    Kulvatunyou, Boonserm; Ivezic, Nenad; Buhwan, Jeong

    2004-07-01

    In this paper, we describe the requirements to test W3C XML Schema usage when defining message schemas for data exchange in any large and evolving enterprise integration project. We then decompose the XML Schema testing into four (4) aspects including the message schema conformance to the XML Schema specification grammar, the message schema conformance to the XML Schema specification semantics, the message schema conformance to design quality testing, and canonical semantics testing of the message schema. We describe these four testing aspects in some detail and point to other related efforts. We further focus to provide some technical details for the message schema design quality testing. As a future work, we describe the requirements for canonical semantics testing and potential solution approaches. Finally, we describe an implementation architecture for the message schema design quality testing.

  8. Design, Specification, and Synthesis of Aircraft Electric Power Systems Control Logic

    NASA Astrophysics Data System (ADS)

    Xu, Huan

    Cyber-physical systems integrate computation, networking, and physical processes. Substantial research challenges exist in the design and verification of such large-scale, distributed sensing, actuation, and control systems. Rapidly improving technology and recent advances in control theory, networked systems, and computer science give us the opportunity to drastically improve our approach to integrated flow of information and cooperative behavior. Current systems rely on text-based specifications and manual design. Using new technology advances, we can create easier, more efficient, and cheaper ways of developing these control systems. This thesis will focus on design considerations for system topologies, ways to formally and automatically specify requirements, and methods to synthesize reactive control protocols, all within the context of an aircraft electric power system as a representative application area. This thesis consists of three complementary parts: synthesis, specification, and design. The first section focuses on the synthesis of central and distributed reactive controllers for an aircraft elec- tric power system. This approach incorporates methodologies from computer science and control. The resulting controllers are correct by construction with respect to system requirements, which are formulated using the specification language of linear temporal logic (LTL). The second section addresses how to formally specify requirements and introduces a domain-specific language for electric power systems. A software tool automatically converts high-level requirements into LTL and synthesizes a controller. The final sections focus on design space exploration. A design methodology is proposed that uses mixed-integer linear programming to obtain candidate topologies, which are then used to synthesize controllers. The discrete-time control logic is then verified in real-time by two methods: hardware and simulation. Finally, the problem of partial observability and

  9. Specific increase in potency via structure-based design of a T cell receptor

    PubMed Central

    Malecek, Karolina; Grigoryan, Arsen; Zhong, Shi; Gu, Wei Jun; Johnson, Laura A.; Rosenberg, Steven A.; Cardozo, Timothy; Krogsgaard, Michelle

    2014-01-01

    Adoptive immunotherapy with antigen-specific T lymphocytes is a powerful strategy for cancer treatment. However, most tumor antigens are non-reactive “self” proteins, which presents an immunotherapy design challenge. Recent studies have shown that tumor-specific T cell receptors (TCRs) can be transduced into normal peripheral blood lymphocytes, which persist after transfer in about 30% of patients and effectively destroy tumor cells in vivo. Although encouraging, the limited clinical responses underscore the need for enrichment of T cells with desirable anti-tumor capabilities prior to patient transfer. In this study, we used structure-based design to predict point mutations of a TCR (DMF5) that enhance its binding affinity for an agonist tumor antigen-major histocompatibility complex (pMHC), Mart-1(27L)-HLA-A2, which elicits full T cell activation to trigger immune responses. We analyzed the effects of selected TCR point mutations on T cell activation potency and analyzed cross-reactivity with related antigens. Our results showed that the mutated TCRs had improved T cell activation potency, while retaining a high degree of specificity. Such affinity-optimized TCRs have demonstrated to be very specific for Mart-1 (27L), the epitope for which they were structurally designed. And even though of limited clinical relevance, these studies open the possibility for future structural-based studies that could potentially be used in adoptive immunotherapy to treat melanoma while avoiding adverse autoimmunity-derived effects. PMID:25070852

  10. Design and component specifications for high average power laser optical systems

    SciTech Connect

    O'Neil, R.W.; Sawicki, R.H.; Johnson, S.A.; Sweatt, W.C.

    1987-01-01

    Laser imaging and transport systems are considered in the regime where laser-induced damage and/or thermal distortion have significant design implications. System design and component specifications are discussed and quantified in terms of the net system transport efficiency and phase budget. Optical substrate materials, figure, surface roughness, coatings, and sizing are considered in the context of visible and near-ir optical systems that have been developed at Lawrence Livermore National Laboratory for laser isotope separation applications. In specific examples of general applicability, details of the bulk and/or surface absorption, peak and/or average power damage threshold, coating characteristics and function, substrate properties, or environmental factors will be shown to drive the component size, placement, and shape in high-power systems. To avoid overstressing commercial fabrication capabilities or component design specifications, procedures will be discussed for compensating for aberration buildup, using a few carefully placed adjustable mirrors. By coupling an aggressive measurements program on substrates and coatings to the design effort, an effective technique has been established to project high-power system performance realistically and, in the process, drive technology developments to improve performance or lower cost in large-scale laser optical systems. 13 refs.

  11. Design and Development of a Model to Simulate 0-G Treadmill Running Using the European Space Agency's Subject Loading System

    NASA Technical Reports Server (NTRS)

    Caldwell, E. C.; Cowley, M. S.; Scott-Pandorf, M. M.

    2010-01-01

    Develop a model that simulates a human running in 0 G using the European Space Agency s (ESA) Subject Loading System (SLS). The model provides ground reaction forces (GRF) based on speed and pull-down forces (PDF). DESIGN The theoretical basis for the Running Model was based on a simple spring-mass model. The dynamic properties of the spring-mass model express theoretical vertical GRF (GRFv) and shear GRF in the posterior-anterior direction (GRFsh) during running gait. ADAMs VIEW software was used to build the model, which has a pelvis, thigh segment, shank segment, and a spring foot (see Figure 1).the model s movement simulates the joint kinematics of a human running at Earth gravity with the aim of generating GRF data. DEVELOPMENT & VERIFICATION ESA provided parabolic flight data of subjects running while using the SLS, for further characterization of the model s GRF. Peak GRF data were fit to a linear regression line dependent on PDF and speed. Interpolation and extrapolation of the regression equation provided a theoretical data matrix, which is used to drive the model s motion equations. Verification of the model was conducted by running the model at 4 different speeds, with each speed accounting for 3 different PDF. The model s GRF data fell within a 1-standard-deviation boundary derived from the empirical ESA data. CONCLUSION The Running Model aids in conducting various simulations (potential scenarios include a fatigued runner or a powerful runner generating high loads at a fast cadence) to determine limitations for the T2 vibration isolation system (VIS) aboard the International Space Station. This model can predict how running with the ESA SLS affects the T2 VIS and may be used for other exercise analyses in the future.

  12. The Environmental Protection Agency: Legislation, Programs and Organization.

    ERIC Educational Resources Information Center

    Environmental Protection Agency, Washington, DC. Library Systems Branch.

    This document is designed to introduce the Environmental Protection Agency (EPA) and its programs. It is organized into four categories which discuss the legislation authorizing EPA activities, the history and present organization, the pollution control programs operated by the Agency, and budgetary appropriations. Specific program…

  13. International Education: A Compendium of Federal Agency Programs.

    ERIC Educational Resources Information Center

    Owens, Becky, Comp.

    Federal agency programs in support of international education are summarized in this report. The publication is designed to help readers discover unknown programs, more fully understand more familiar programs, and learn more about specific requirements for agencies where proposals have been unsuccessfully submitted in the past. Focus is directed…

  14. Quantification of the transferability of a designed protein specificity switch reveals extensive epistasis in molecular recognition

    DOE PAGES

    Melero, Cristina; Ollikainen, Noah; Harwood, Ian; Karpiak, Joel; Kortemme, Tanja

    2014-10-13

    Re-engineering protein–protein recognition is an important route to dissecting and controlling complex interaction networks. Experimental approaches have used the strategy of “second-site suppressors,” where a functional interaction is inferred between two proteins if a mutation in one protein can be compensated by a mutation in the second. Mimicking this strategy, computational design has been applied successfully to change protein recognition specificity by predicting such sets of compensatory mutations in protein–protein interfaces. To extend this approach, it would be advantageous to be able to “transplant” existing engineered and experimentally validated specificity changes to other homologous protein–protein complexes. Here, we test thismore » strategy by designing a pair of mutations that modulates peptide recognition specificity in the Syntrophin PDZ domain, confirming the designed interaction biochemically and structurally, and then transplanting the mutations into the context of five related PDZ domain–peptide complexes. We find a wide range of energetic effects of identical mutations in structurally similar positions, revealing a dramatic context dependence (epistasis) of designed mutations in homologous protein–protein interactions. To better understand the structural basis of this context dependence, we apply a structure-based computational model that recapitulates these energetic effects and we use this model to make and validate forward predictions. The context dependence of these mutations is captured by computational predictions, our results both highlight the considerable difficulties in designing protein–protein interactions and provide challenging benchmark cases for the development of improved protein modeling and design methods that accurately account for the context.« less

  15. Quantification of the transferability of a designed protein specificity switch reveals extensive epistasis in molecular recognition

    SciTech Connect

    Melero, Cristina; Ollikainen, Noah; Harwood, Ian; Karpiak, Joel; Kortemme, Tanja

    2014-10-13

    Re-engineering protein–protein recognition is an important route to dissecting and controlling complex interaction networks. Experimental approaches have used the strategy of “second-site suppressors,” where a functional interaction is inferred between two proteins if a mutation in one protein can be compensated by a mutation in the second. Mimicking this strategy, computational design has been applied successfully to change protein recognition specificity by predicting such sets of compensatory mutations in protein–protein interfaces. To extend this approach, it would be advantageous to be able to “transplant” existing engineered and experimentally validated specificity changes to other homologous protein–protein complexes. Here, we test this strategy by designing a pair of mutations that modulates peptide recognition specificity in the Syntrophin PDZ domain, confirming the designed interaction biochemically and structurally, and then transplanting the mutations into the context of five related PDZ domain–peptide complexes. We find a wide range of energetic effects of identical mutations in structurally similar positions, revealing a dramatic context dependence (epistasis) of designed mutations in homologous protein–protein interactions. To better understand the structural basis of this context dependence, we apply a structure-based computational model that recapitulates these energetic effects and we use this model to make and validate forward predictions. The context dependence of these mutations is captured by computational predictions, our results both highlight the considerable difficulties in designing protein–protein interactions and provide challenging benchmark cases for the development of improved protein modeling and design methods that accurately account for the context.

  16. Quantification of the transferability of a designed protein specificity switch reveals extensive epistasis in molecular recognition.

    PubMed

    Melero, Cristina; Ollikainen, Noah; Harwood, Ian; Karpiak, Joel; Kortemme, Tanja

    2014-10-28

    Reengineering protein-protein recognition is an important route to dissecting and controlling complex interaction networks. Experimental approaches have used the strategy of "second-site suppressors," where a functional interaction is inferred between two proteins if a mutation in one protein can be compensated by a mutation in the second. Mimicking this strategy, computational design has been applied successfully to change protein recognition specificity by predicting such sets of compensatory mutations in protein-protein interfaces. To extend this approach, it would be advantageous to be able to "transplant" existing engineered and experimentally validated specificity changes to other homologous protein-protein complexes. Here, we test this strategy by designing a pair of mutations that modulates peptide recognition specificity in the Syntrophin PDZ domain, confirming the designed interaction biochemically and structurally, and then transplanting the mutations into the context of five related PDZ domain-peptide complexes. We find a wide range of energetic effects of identical mutations in structurally similar positions, revealing a dramatic context dependence (epistasis) of designed mutations in homologous protein-protein interactions. To better understand the structural basis of this context dependence, we apply a structure-based computational model that recapitulates these energetic effects and we use this model to make and validate forward predictions. Although the context dependence of these mutations is captured by computational predictions, our results both highlight the considerable difficulties in designing protein-protein interactions and provide challenging benchmark cases for the development of improved protein modeling and design methods that accurately account for the context.

  17. Region and site specific design guidelines for the northeastern Venezuela development

    SciTech Connect

    Gajardo, E.; Paga, M.; Sully, J.P.

    1996-12-31

    Several site specific studies were performed for the northeastern Venezuelan offshore development in order to determine the environmental parameters for the engineering design. The geotechnical parameters were obtained from the analysis of the soil borings, in situ tests and the high resolution seismic surveys. An evaluation of the seismic hazard was done, based on a detailed neotectonic study, the compilation of historical and instrumental seismological information, and an attenuation relationship developed for the studied area. Seismic response analyses were performed using the seismic parameters and dynamic characteristics obtained form the soil borings and in situ tests of shear waves. The met-ocean design loads of the local conditions were obtained from mathematical modeling based on in situ and regional measurements. This study defines the platforms design conditions based on a probabilistic reliability approach and it has been used, among others, as a basis for the earthquake design guidelines proposed by the International Standards Organization. A sensitivity analysis was performed starting from the uncertainties of all the input parameters, in order to obtain the best estimate average design spectrum and associated coefficient of variability. The specific approach was to maintain the same safety index adopted by API RP2A code, with a bias modification factor that relates the site nominal spectra with the spectral acceleration that would be used in order to satisfy the API requirements. The results of this approach make possible the use of site specific detailed environmental data and the proven standard procedures established on the API RP2 codes, with significant cost reduction in terms of both platform and foundation design. The considerable amount of tectonic, seismological, and geotechnical information was the principal factor allowing the above methodology.

  18. Quantification of the transferability of a designed protein specificity switch reveals extensive epistasis in molecular recognition.

    PubMed

    Melero, Cristina; Ollikainen, Noah; Harwood, Ian; Karpiak, Joel; Kortemme, Tanja

    2014-10-28

    Reengineering protein-protein recognition is an important route to dissecting and controlling complex interaction networks. Experimental approaches have used the strategy of "second-site suppressors," where a functional interaction is inferred between two proteins if a mutation in one protein can be compensated by a mutation in the second. Mimicking this strategy, computational design has been applied successfully to change protein recognition specificity by predicting such sets of compensatory mutations in protein-protein interfaces. To extend this approach, it would be advantageous to be able to "transplant" existing engineered and experimentally validated specificity changes to other homologous protein-protein complexes. Here, we test this strategy by designing a pair of mutations that modulates peptide recognition specificity in the Syntrophin PDZ domain, confirming the designed interaction biochemically and structurally, and then transplanting the mutations into the context of five related PDZ domain-peptide complexes. We find a wide range of energetic effects of identical mutations in structurally similar positions, revealing a dramatic context dependence (epistasis) of designed mutations in homologous protein-protein interactions. To better understand the structural basis of this context dependence, we apply a structure-based computational model that recapitulates these energetic effects and we use this model to make and validate forward predictions. Although the context dependence of these mutations is captured by computational predictions, our results both highlight the considerable difficulties in designing protein-protein interactions and provide challenging benchmark cases for the development of improved protein modeling and design methods that accurately account for the context. PMID:25313039

  19. Design of specific hardware to obtain embryos and maintain adult urodele amphibians aboard a space station

    NASA Astrophysics Data System (ADS)

    Husson, D.; Chaput, D.; Bautz, A.; Davet, J.; Durand, D.; Dournon, C.; Duprat, A. M.; Gualandris-Parisot, L.

    The study of the influence of weightlessness on fertilization and embryonic development of a vertebrate is of importance in the understanding of basic embryogenesis and in the preparation of the future exploration of space. Accordingly, specific hardware was designed to perform experiments on board the MIR space station with an amphibian vertebrate model, taking into account the biological requirements and the multiple constraints of a longterm space mission. This paper describes the biological uses and presents the technological specifications of the device developed under CNES management. The hardware was adapted to and is compatible with biological requirements as confirmed by three experiments performed in space on board the orbital MIR station.

  20. Design of specific hardware to obtain embryos and maintain adult urodele amphibians aboard a space station.

    PubMed

    Husson, D; Chaput, D; Bautz, A; Davet, J; Durand, D; Dournon, C; Duprat, A M; Gualandris-Parisot, L

    2001-01-01

    The study of the influence of weightlessness on fertilization and embryonic development of a vertebrate is of importance in the understanding of basic embryogenesis and in the preparation of the future exploration of space. Accordingly, specific hardware was designed to perform experiments on board the MIR space station with an amphibian vertebrate model, taking into account the biological requirements and the multiple constraints of a long-term mission. This paper describes the biological uses and presents the technological specifications of the device developed under CNES management. The hardware was adapted to and is compatible with biological requirements as confirmed by three experiments performed in space on board the orbital MIR station.

  1. MethMarker: user-friendly design and optimization of gene-specific DNA methylation assays

    PubMed Central

    2009-01-01

    DNA methylation is a key mechanism of epigenetic regulation that is frequently altered in diseases such as cancer. To confirm the biological or clinical relevance of such changes, gene-specific DNA methylation changes need to be validated in multiple samples. We have developed the MethMarker http://methmarker.mpi-inf.mpg.de/ software to help design robust and cost-efficient DNA methylation assays for six widely used methods. Furthermore, MethMarker implements a bioinformatic workflow for transforming disease-specific differentially methylated genomic regions into robust clinical biomarkers. PMID:19804638

  2. Block 4 solar cell module design and test specification for residential applications

    NASA Technical Reports Server (NTRS)

    1978-01-01

    Near-term design, qualification and acceptance requirements are provided for terrestrial solar cell modules suitable for incorporation in photovoltaic power sources (2 kW to 10 kW) applied to single family residential installations. Requirement levels and recommended design limits for selected performance criteria are specified for modules intended principally for rooftop installations. Modules satisfying the requirements of this specification fall into one of two categories, residential panel or residential shingle, both meeting general performance requirements plus additional category peculiar constraints.

  3. A preliminary report of designing removable partial denture frameworks using a specifically developed software package.

    PubMed

    Han, Jing; Wang, Yong; Lü, Peijun

    2010-01-01

    This article reports on a method to digitally survey and build virtual patterns for removable partial denture (RPD) frameworks using a new three-dimensional (3D) computer-aided design/computer-assisted manufacturing (CAD/CAM) software package developed specifically for RPD design. The procedure included obtaining 3D data from partially dentate casts, deciding on the path of insertion, and modeling the shape of the components of the frameworks digitally. The completed model data were stored as stereolithography (STL) files, which are commonly used in transferring CAD/CAM models to rapid prototyping technologies. Finally, metal RPD frameworks were fabricated using a selective laser melting technique.

  4. Mod-5A wind turbine generator program design report. Volume 4: Drawings and specifications, book 4

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The design, development and analysis of the 7.3 MW MOD-5A wind turbine generator are documented. There are four volumes. This volume contains the drawings and specifications that were developed in preparation for building the MOD-5A wind turbine generator. This volume contains 5 books of which this is the fourth, providing drawings 47A380128 through 47A387125. In addition to the parts listing and where-used list, the logic design of the controller software and the code listing of the controller software are provided. Also given are the aerodynamic profile coordinates.

  5. SLS-SPEC-159 Cross-Program Design Specification for Natural Environments (DSNE) Revision D

    NASA Technical Reports Server (NTRS)

    Roberts, Barry C.

    2015-01-01

    This document is derived from the former National Aeronautics and Space Administration (NASA) Constellation Program (CxP) document CxP 70023, titled "The Design Specification for Natural Environments (DSNE), Revision C." The original document has been modified to represent updated Design Reference Missions (DRMs) for the NASA Exploration Systems Development (ESD) Programs. The DSNE completes environment-related specifications for architecture, system-level, and lower-tier documents by specifying the ranges of environmental conditions that must be accounted for by NASA ESD Programs. To assure clarity and consistency, and to prevent requirements documents from becoming cluttered with extensive amounts of technical material, natural environment specifications have been compiled into this document. The intent is to keep a unified specification for natural environments that each Program calls out for appropriate application. This document defines the natural environments parameter limits (maximum and minimum values, energy spectra, or precise model inputs, assumptions, model options, etc.), for all ESD Programs. These environments are developed by the NASA Marshall Space Flight Center (MSFC) Natural Environments Branch (MSFC organization code: EV44). Many of the parameter limits are based on experience with previous programs, such as the Space Shuttle Program. The parameter limits contain no margin and are meant to be evaluated individually to ensure they are reasonable (i.e., do not apply unrealistic extreme-on-extreme conditions). The natural environments specifications in this document should be accounted for by robust design of the flight vehicle and support systems. However, it is understood that in some cases the Programs will find it more effective to account for portions of the environment ranges by operational mitigation or acceptance of risk in accordance with an appropriate program risk management plan and/or hazard analysis process. The DSNE is not intended

  6. Computational model design specification for Phase 1 of the Hanford Environmental Dose Reconstruction Project

    SciTech Connect

    Napier, B.A.

    1991-07-01

    The objective of the Hanford Environmental Dose Reconstruction (HEDR) Project is to estimate the radiation dose that individuals could have received as a result of emission from nuclear operations at Hanford since their inception in 1944. The purpose of this report is to outline the basic algorithm and necessary computer calculations to be used to calculate radiation doses specific and hypothetical individuals in the vicinity of Hanford. The system design requirements, those things that must be accomplished, are defined. The system design specifications, the techniques by which those requirements are met, are outlined. Included are the basic equations, logic diagrams, and preliminary definition of the nature of each input distribution. 4 refs., 10 figs., 9 tabs.

  7. Biomechanical Assessment of a Patient-Specific Knee Implant Design Using Finite Element Method

    PubMed Central

    Bai, Jianfeng; Huang, Yongling; Lin, Jianhao

    2014-01-01

    Rheumatoid arthritis is the leading cause of disability in young adults. Total knee arthroplasty has been successfully used to restore the joint function. Due to small bone size, osteoporosis, and severe soft tissue disease, standard knee implant sometimes cannot be directly applied clinically and patient-specific designs may be a more rational choice. The purpose of this study was to evaluate the biomechanical behavior of a patient-specific knee implant. A three-dimensional finite element of total knee arthroplasty was developed. The mechanical strength and the wear damage of the articular surfaces were analyzed. The results show that there exist high risks of component fracture and wear damage; the proposed implant design should be abandoned. The presurgery analysis is helpful in avoiding the potential failure. PMID:25101275

  8. Tools to aid the specification and design of flight software, appendix B

    NASA Technical Reports Server (NTRS)

    Bristow, G.

    1980-01-01

    The tasks that are normally performed during the specification and architecture design stages of software development are identified. Ways that tools could perform, or aid the performance, of such tasks are also identified. Much of the verification and analysis that is suggested is currently rarely performed during these early stages, but it is believed that this analysis should be done as early as possible so as to detect errors as early as possible.

  9. Design and implementation of a domain specific language for phylogenetic inference.

    PubMed

    Pontelli, Enrico; Ranjan, Desh; Gupta, Gopal; Milligan, Brook

    2003-07-01

    Domain experts think and reason at a high level of abstraction when they solve problems in their domain of expertise. We present the design and motivation behind a domain specific language, called phi LOG, to enable biologists to program solutions to phylogenetic inference problems at a very high level of abstraction. The implementation infrastructure (interpreter, compiler, debugger) for the DSL is automatically obtained through a software engineering framework based on Denotational Semantics and Logic Programming.

  10. Computational design of the affinity and specificity of a therapeutic T cell receptor.

    PubMed

    Pierce, Brian G; Hellman, Lance M; Hossain, Moushumi; Singh, Nishant K; Vander Kooi, Craig W; Weng, Zhiping; Baker, Brian M

    2014-02-01

    T cell receptors (TCRs) are key to antigen-specific immunity and are increasingly being explored as therapeutics, most visibly in cancer immunotherapy. As TCRs typically possess only low-to-moderate affinity for their peptide/MHC (pMHC) ligands, there is a recognized need to develop affinity-enhanced TCR variants. Previous in vitro engineering efforts have yielded remarkable improvements in TCR affinity, yet concerns exist about the maintenance of peptide specificity and the biological impacts of ultra-high affinity. As opposed to in vitro engineering, computational design can directly address these issues, in theory permitting the rational control of peptide specificity together with relatively controlled increments in affinity. Here we explored the efficacy of computational design with the clinically relevant TCR DMF5, which recognizes nonameric and decameric epitopes from the melanoma-associated Melan-A/MART-1 protein presented by the class I MHC HLA-A2. We tested multiple mutations selected by flexible and rigid modeling protocols, assessed impacts on affinity and specificity, and utilized the data to examine and improve algorithmic performance. We identified multiple mutations that improved binding affinity, and characterized the structure, affinity, and binding kinetics of a previously reported double mutant that exhibits an impressive 400-fold affinity improvement for the decameric pMHC ligand without detectable binding to non-cognate ligands. The structure of this high affinity mutant indicated very little conformational consequences and emphasized the high fidelity of our modeling procedure. Overall, our work showcases the capability of computational design to generate TCRs with improved pMHC affinities while explicitly accounting for peptide specificity, as well as its potential for generating TCRs with customized antigen targeting capabilities.

  11. Experiences with the hydraulic design of the high specific speed Francis turbine

    NASA Astrophysics Data System (ADS)

    Obrovsky, J.; Zouhar, J.

    2014-03-01

    The high specific speed Francis turbine is still suitable alternative for refurbishment of older hydro power plants with lower heads and worse cavitation conditions. In the paper the design process of such kind of turbine together with the results comparison of homological model tests performed in hydraulic laboratory of ČKD Blansko Engineering is introduced. The turbine runner was designed using the optimization algorithm and considering the high specific speed hydraulic profile. It means that hydraulic profiles of the spiral case, the distributor and the draft tube were used from a Kaplan turbine. The optimization was done as the automatic cycle and was based on a simplex optimization method as well as on a genetic algorithm. The number of blades is shown as the parameter which changes the resulting specific speed of the turbine between ns=425 to 455 together with the cavitation characteristics. Minimizing of cavitation on the blade surface as well as on the inlet edge of the runner blade was taken into account during the design process. The results of CFD analyses as well as the model tests are mentioned in the paper.

  12. A situational approach to the design of a patient-oriented disease-specific knowledge base.

    PubMed Central

    Kim, Matthew I.; Ladenson, Paul; Johnson, Kevin B.

    2002-01-01

    We have developed a situational approach to the organization of disease-specific information that seeks to provide patients with targeted access to content in a knowledge base. Our approach focuses on dividing a defined knowledge base into sections corresponding to discrete clinical events associated with the evaluation and treatment of a specific disorder. Common reasons for subspecialty referral are used to generate situational statements that serve as entry points into the knowledge base. Each section includes defining questions generated using keywords associated with specific topics. Defining questions are linked to patient-focused answers. Evaluation of a thyroid cancer web site designed using this approach has identified high ratings for usability, relevance, and comprehension of retrieved information. This approach may be particularly useful in the development of resources for newly diagnosed patients. PMID:12463852

  13. Structure-based Design of Peptides with High Affinity and Specificity to HER2 Positive Tumors

    PubMed Central

    Geng, Lingling; Wang, Zihua; Yang, Xiaoliang; Li, Dan; Lian, Wenxi; Xiang, Zhichu; Wang, Weizhi; Bu, Xiangli; Lai, Wenjia; Hu, Zhiyuan; Fang, Qiaojun

    2015-01-01

    To identify peptides with high affinity and specificity against human epidermal growth factor receptor 2 (HER2), a series of peptides were designed based on the structure of HER2 and its Z(HER2:342) affibody. By using a combination protocol of molecular dynamics modeling, MM/GBSA binding free energy calculations, and binding free energy decomposition analysis, two novel peptides with 27 residues, pep27 and pep27-24M, were successfully obtained. Immunocytochemistry and flow cytometry analysis verified that both peptides can specifically bind to the extracellular domain of HER2 protein at cellular level. The Surface Plasmon Resonance imaging (SPRi) analysis showed that dissociation constants (KD) of these two peptides were around 300 nmol/L. Furthermore, fluorescence imaging of peptides against nude mice xenografted with SKBR3 cells indicated that both peptides have strong affinity and high specificity to HER2 positive tumors. PMID:26284145

  14. Engineering Bacteria to Search for Specific Concentrations of Molecules by a Systematic Synthetic Biology Design Method.

    PubMed

    Tien, Shin-Ming; Hsu, Chih-Yuan; Chen, Bor-Sen

    2016-01-01

    Bacteria navigate environments full of various chemicals to seek favorable places for survival by controlling the flagella's rotation using a complicated signal transduction pathway. By influencing the pathway, bacteria can be engineered to search for specific molecules, which has great potential for application to biomedicine and bioremediation. In this study, genetic circuits were constructed to make bacteria search for a specific molecule at particular concentrations in their environment through a synthetic biology method. In addition, by replacing the "brake component" in the synthetic circuit with some specific sensitivities, the bacteria can be engineered to locate areas containing specific concentrations of the molecule. Measured by the swarm assay qualitatively and microfluidic techniques quantitatively, the characteristics of each "brake component" were identified and represented by a mathematical model. Furthermore, we established another mathematical model to anticipate the characteristics of the "brake component". Based on this model, an abundant component library can be established to provide adequate component selection for different searching conditions without identifying all components individually. Finally, a systematic design procedure was proposed. Following this systematic procedure, one can design a genetic circuit for bacteria to rapidly search for and locate different concentrations of particular molecules by selecting the most adequate "brake component" in the library. Moreover, following simple procedures, one can also establish an exclusive component library suitable for other cultivated environments, promoter systems, or bacterial strains.

  15. Engineering Bacteria to Search for Specific Concentrations of Molecules by a Systematic Synthetic Biology Design Method

    PubMed Central

    Chen, Bor-Sen

    2016-01-01

    Bacteria navigate environments full of various chemicals to seek favorable places for survival by controlling the flagella’s rotation using a complicated signal transduction pathway. By influencing the pathway, bacteria can be engineered to search for specific molecules, which has great potential for application to biomedicine and bioremediation. In this study, genetic circuits were constructed to make bacteria search for a specific molecule at particular concentrations in their environment through a synthetic biology method. In addition, by replacing the “brake component” in the synthetic circuit with some specific sensitivities, the bacteria can be engineered to locate areas containing specific concentrations of the molecule. Measured by the swarm assay qualitatively and microfluidic techniques quantitatively, the characteristics of each “brake component” were identified and represented by a mathematical model. Furthermore, we established another mathematical model to anticipate the characteristics of the “brake component”. Based on this model, an abundant component library can be established to provide adequate component selection for different searching conditions without identifying all components individually. Finally, a systematic design procedure was proposed. Following this systematic procedure, one can design a genetic circuit for bacteria to rapidly search for and locate different concentrations of particular molecules by selecting the most adequate “brake component” in the library. Moreover, following simple procedures, one can also establish an exclusive component library suitable for other cultivated environments, promoter systems, or bacterial strains. PMID:27096615

  16. MODIS Information, Data, and Control System (MIDACS) system specifications and conceptual design

    NASA Technical Reports Server (NTRS)

    Han, D.; Salomonson, V.; Ormsby, J.; Ardanuy, P.; Mckay, A.; Hoyt, D.; Jaffin, S.; Vallette, B.; Sharts, B.; Folta, D.

    1988-01-01

    The MODIS Information, Data, and Control System (MIDACS) Specifications and Conceptual Design Document discusses system level requirements, the overall operating environment in which requirements must be met, and a breakdown of MIDACS into component subsystems, which include the Instrument Support Terminal, the Instrument Control Center, the Team Member Computing Facility, the Central Data Handling Facility, and the Data Archive and Distribution System. The specifications include sizing estimates for the processing and storage capacities of each data system element, as well as traffic analyses of data flows between the elements internally, and also externally across the data system interfaces. The specifications for the data system, as well as for the individual planning and scheduling, control and monitoring, data acquisition and processing, calibration and validation, and data archive and distribution components, do not yet fully specify the data system in the complete manner needed to achieve the scientific objectives of the MODIS instruments and science teams. The teams have not yet been formed; however, it was possible to develop the specifications and conceptual design based on the present concept of EosDIS, the Level-1 and Level-2 Functional Requirements Documents, the Operations Concept, and through interviews and meetings with key members of the scientific community.

  17. Designing Specification Languages for Process Control Systems: Lessons Learned and Steps to the Future

    NASA Technical Reports Server (NTRS)

    Leveson, Nancy G.; Heimdahl, Mats P. E.; Reese, Jon Damon

    1999-01-01

    Previously, we defined a blackbox formal system modeling language called RSML (Requirements State Machine Language). The language was developed over several years while specifying the system requirements for a collision avoidance system for commercial passenger aircraft. During the language development, we received continual feedback and evaluation by FAA employees and industry representatives, which helped us to produce a specification language that is easily learned and used by application experts. Since the completion of the PSML project, we have continued our research on specification languages. This research is part of a larger effort to investigate the more general problem of providing tools to assist in developing embedded systems. Our latest experimental toolset is called SpecTRM (Specification Tools and Requirements Methodology), and the formal specification language is SpecTRM-RL (SpecTRM Requirements Language). This paper describes what we have learned from our use of RSML and how those lessons were applied to the design of SpecTRM-RL. We discuss our goals for SpecTRM-RL and the design features that support each of these goals.

  18. Towards the formal specification of the requirements and design of a processor interface unit

    NASA Technical Reports Server (NTRS)

    Fura, David A.; Windley, Phillip J.; Cohen, Gerald C.

    1993-01-01

    Work to formally specify the requirements and design of a Processor Interface Unit (PIU), a single-chip subsystem providing memory interface, bus interface, and additional support services for a commercial microprocessor within a fault-tolerant computer system, is described. This system, the Fault-Tolerant Embedded Processor (FTEP), is targeted towards applications in avionics and space requiring extremely high levels of mission reliability, extended maintenance free operation, or both. The approaches that were developed for modeling the PIU requirements and for composition of the PIU subcomponents at high levels of abstraction are described. These approaches were used to specify and verify a nontrivial subset of the PIU behavior. The PIU specification in Higher Order Logic (HOL) is documented in a companion NASA contractor report entitled 'Towards the Formal Specification of the Requirements and Design of a Processor Interfacs Unit - HOL Listings.' The subsequent verification approach and HOL listings are documented in NASA contractor report entitled 'Towards the Formal Verification of the Requirements and Design of a Processor Interface Unit' and NASA contractor report entitled 'Towards the Formal Verification of the Requirements and Design of a Processor Interface Unit - HOL Listings.'

  19. Topological optimization for designing patient-specific large craniofacial segmental bone replacements

    PubMed Central

    Sutradhar, Alok; Paulino, Glaucio H.; Miller, Michael J.; Nguyen, Tam H.

    2010-01-01

    Restoring normal function and appearance after massive facial injuries with bone loss is an important unsolved problem in surgery. An important limitation of the current methods is heuristic ad hoc design of bone replacements by the operating surgeon at the time of surgery. This problem might be addressed by incorporating a computational method known as topological optimization into routine surgical planning. We tested the feasibility of using a multiresolution three-dimensional topological optimization to design replacements for massive midface injuries with bone loss. The final solution to meet functional requirements may be shaped differently than the natural human bone but be optimized for functional needs sufficient to support full restoration using a combination of soft tissue repair and synthetic prosthetics. Topological optimization for designing facial bone tissue replacements might improve current clinical methods and provide essential enabling technology to translate generic bone tissue engineering methods into specific solutions for individual patients. PMID:20628014

  20. System specification/system design document comment review: Plutonium Stabilization and Packaging System. Notes of conference

    SciTech Connect

    1996-07-01

    A meeting was held between DOE personnel and the BNFL team to review the proposed resolutions to DOE comments on the initial issue of the system specification and system design document for the Plutonium Stabilization and Packaging System. The objectives of this project are to design, fabricate, install, and start up a glovebox system for the safe repackaging of plutonium oxide and metal, with a requirement of a 50-year storage period. The areas discussed at the meeting were: nitrogen in can; moisture instrumentation; glovebox atmosphere; can marking-bar coding; weld quality; NFPA-101 references; inner can swabbing; ultimate storage environment; throughput; convenience can screw-top design; furnace/trays; authorization basis; compactor safety; schedule for DOE review actions; fire protection; criticality safety; applicable standards; approach to MC and A; homogeneous oxide; resistance welder power; and tray overfill. Revised resolutions were drafted and are presented.

  1. Earth Observatory Satellite system definition study. Report no. 5: System design and specifications. Part 2: Ground system element specifications

    NASA Technical Reports Server (NTRS)

    1974-01-01

    The Ground System requirements for the Land Resources Management (LRM) type-A and type-B missions of the Earth Observatory Satellite (EOS) program are presented. Specifications for the Thematic Mapper data processing are provided (LRM A mission). The specifications also cover the R and D instruments (Thematic Mapper and High Resolution Pointable Imager) data processing for the LRM type-B mission.

  2. Application-specific coarse-grained reconfigurable array: architecture and design methodology

    NASA Astrophysics Data System (ADS)

    Zhou, Li; Liu, Dongpei; Zhang, Jianfeng; Liu, Hengzhu

    2015-06-01

    Coarse-grained reconfigurable arrays (CGRAs) have shown potential for application in embedded systems in recent years. Numerous reconfigurable processing elements (PEs) in CGRAs provide flexibility while maintaining high performance by exploring different levels of parallelism. However, a difference remains between the CGRA and the application-specific integrated circuit (ASIC). Some application domains, such as software-defined radios (SDRs), require flexibility with performance demand increases. More effective CGRA architectures are expected to be developed. Customisation of a CGRA according to its application can improve performance and efficiency. This study proposes an application-specific CGRA architecture template composed of generic PEs (GPEs) and special PEs (SPEs). The hardware of the SPE can be customised to accelerate specific computational patterns. An automatic design methodology that includes pattern identification and application-specific function unit generation is also presented. A mapping algorithm based on ant colony optimisation is provided. Experimental results on the SDR target domain show that compared with other ordinary and application-specific reconfigurable architectures, the CGRA generated by the proposed method performs more efficiently for given applications.

  3. CD4-Specific Designed Ankyrin Repeat Proteins Are Novel Potent HIV Entry Inhibitors with Unique Characteristics

    PubMed Central

    Schweizer, Andreas; Rusert, Peter; Berlinger, Livia; Ruprecht, Claudia R.; Mann, Axel; Corthésy, Stéphanie; Turville, Stuart G.; Aravantinou, Meropi; Fischer, Marek; Robbiani, Melissa; Amstutz, Patrick; Trkola, Alexandra

    2008-01-01

    Here, we describe the generation of a novel type of HIV entry inhibitor using the recently developed Designed Ankyrin Repeat Protein (DARPin) technology. DARPin proteins specific for human CD4 were selected from a DARPin DNA library using ribosome display. Selected pool members interacted specifically with CD4 and competed with gp120 for binding to CD4. DARPin proteins derived in the initial selection series inhibited HIV in a dose-dependent manner, but showed a relatively high variability in their capacity to block replication of patient isolates on primary CD4 T cells. In consequence, a second series of CD4-specific DARPins with improved affinity for CD4 was generated. These 2nd series DARPins potently inhibit infection of genetically divergent (subtype B and C) HIV isolates in the low nanomolar range, independent of coreceptor usage. Importantly, the actions of the CD4 binding DARPins were highly specific: no effect on cell viability or activation, CD4 memory cell function, or interference with CD4-independent virus entry was observed. These novel CD4 targeting molecules described here combine the unique characteristics of DARPins—high physical stability, specificity and low production costs—with the capacity to potently block HIV entry, rendering them promising candidates for microbicide development. PMID:18654624

  4. Solid Waste Operations Complex W-113, Detail Design Report (Title II). Volume 3: Specifications

    SciTech Connect

    1995-09-01

    The Solid Waste Retrieval Facility--Phase 1 (Project W113) will provide the infrastructure and the facility required to retrieve from Trench 04, Burial ground 4C, contact handled (CH) drums and boxes at a rate that supports all retrieved TRU waste batching, treatment, storage, and disposal plans. This includes (1) operations related equipment and facilities, viz., a weather enclosure for the trench, retrieval equipment, weighing, venting, obtaining gas samples, overpacking, NDE, NDA, shipment of waste and (2) operations support related facilities, viz., a general office building, a retrieval staff change facility, and infrastructure upgrades such as supply and routing of water, sewer, electrical power, fire protection, roads, and telecommunication. Title I design for the operations related equipment and facilities was performed by Raytheon/BNFL, and that for the operations support related facilities including infrastructure upgrade was performed by KEH. These two scopes were combined into an integrated W113 Title II scope that was performed by Raytheon/BNFL. Volume 3 is a compilation of the construction specifications that will constitute the Title II materials and performance specifications. This volume contains CSI specifications for non-equipment related construction material type items, performance type items, and facility mechanical equipment items. Data sheets are provided, as necessary, which specify the equipment overall design parameters.

  5. Investigation into Deep Brain Stimulation Lead Designs: A Patient-Specific Simulation Study.

    PubMed

    Alonso, Fabiola; Latorre, Malcolm A; Göransson, Nathanael; Zsigmond, Peter; Wårdell, Karin

    2016-01-01

    New deep brain stimulation (DBS) electrode designs offer operation in voltage and current mode and capability to steer the electric field (EF). The aim of the study was to compare the EF distributions of four DBS leads at equivalent amplitudes (3 V and 3.4 mA). Finite element method (FEM) simulations (n = 38) around cylindrical contacts (leads 3389, 6148) or equivalent contact configurations (leads 6180, SureStim1) were performed using homogeneous and patient-specific (heterogeneous) brain tissue models. Steering effects of 6180 and SureStim1 were compared with symmetric stimulation fields. To make relative comparisons between simulations, an EF isolevel of 0.2 V/mm was chosen based on neuron model simulations (n = 832) applied before EF visualization and comparisons. The simulations show that the EF distribution is largely influenced by the heterogeneity of the tissue, and the operating mode. Equivalent contact configurations result in similar EF distributions. In steering configurations, larger EF volumes were achieved in current mode using equivalent amplitudes. The methodology was demonstrated in a patient-specific simulation around the zona incerta and a "virtual" ventral intermediate nucleus target. In conclusion, lead design differences are enhanced when using patient-specific tissue models and current stimulation mode. PMID:27618109

  6. Rational design of an estrogen receptor mutant with altered DNA-binding specificity

    PubMed Central

    Nguyen, Denis; Bail, Martine; Pesant, Genevieve; Dupont, Virginie N.; Rouault, Étienne; Deschênes, Julie; Rocha, Walter; Melançon, Geneviève; Steinberg, Sergey V.; Mader, Sylvie

    2007-01-01

    Although artificial C2-H2 zinc fingers can be designed to recognize specific DNA sequences, it remains unclear to which extent nuclear receptor C4 zinc fingers can be tailored to bind novel DNA elements. Steroid receptors bind as dimers to palindromic response elements differing in the two central base pairs of repeated motifs. Predictions based on one amino acid—one base-pair relationships may not apply to estrogen receptors (ERs), which recognize the two central base pairs of estrogen response elements (EREs) via two charged amino acids, each contacting two bases on opposite DNA strands. Mutagenesis of these residues, E203 and K210 in ERα, indicated that both contribute to ERE binding. Removal of the electric charge and steric constraints associated with K210 was required for full loss of parental DNA-binding specificity and recognition of novel sequences by E203 mutants. Although some of the new binding profiles did not match predictions, the double mutation E203R-K210A generated as predicted a mutant ER that was transcriptionally active on palindromes of PuGCTCA motifs, but not on consensus EREs. This study demonstrates the feasibility of designing C4 zinc finger mutants with novel DNA-binding specificity, but also uncovers limitations of this approach. PMID:17478511

  7. Investigation into Deep Brain Stimulation Lead Designs: A Patient-Specific Simulation Study

    PubMed Central

    Alonso, Fabiola; Latorre, Malcolm A.; Göransson, Nathanael; Zsigmond, Peter; Wårdell, Karin

    2016-01-01

    New deep brain stimulation (DBS) electrode designs offer operation in voltage and current mode and capability to steer the electric field (EF). The aim of the study was to compare the EF distributions of four DBS leads at equivalent amplitudes (3 V and 3.4 mA). Finite element method (FEM) simulations (n = 38) around cylindrical contacts (leads 3389, 6148) or equivalent contact configurations (leads 6180, SureStim1) were performed using homogeneous and patient-specific (heterogeneous) brain tissue models. Steering effects of 6180 and SureStim1 were compared with symmetric stimulation fields. To make relative comparisons between simulations, an EF isolevel of 0.2 V/mm was chosen based on neuron model simulations (n = 832) applied before EF visualization and comparisons. The simulations show that the EF distribution is largely influenced by the heterogeneity of the tissue, and the operating mode. Equivalent contact configurations result in similar EF distributions. In steering configurations, larger EF volumes were achieved in current mode using equivalent amplitudes. The methodology was demonstrated in a patient-specific simulation around the zona incerta and a “virtual” ventral intermediate nucleus target. In conclusion, lead design differences are enhanced when using patient-specific tissue models and current stimulation mode. PMID:27618109

  8. Specific selection criteria and testing protocol optimize reservoir drill-in fluid design

    SciTech Connect

    Donovan, J.P.; Jones, T.A.

    1995-12-31

    Proper test and evaluation methodology for reservoir drill-in fluids is an important step in the construction design of a producing wellbore. Drill-in fluids are specifically designed to meet drilling and completion objectives, simplify reservoir cleanup and maximize production rates in open hole completions. New protocols developed by drilling fluid and completion laboratories have proven to be effective tools for evaluating fluid designs that optimize production. Drill-in fluid selection begins with an initial screening process taking into account qualifying issues such as environmental acceptability, reservoir temperature, physical limitations, and chemical compatibility between the fluid and the reservoir. The final fluid formulation should minimize formation damage, fluid leak-off rates, breakout pressures, and wellbore cleanup of drill-in and completion operations, thus maximizing the productivity of the well. Formation damage risks can be minimized if laboratory testing procedures parallel specific reservoir conditions. Case studies confirm that a methodology based on laboratory data that incorporates drilling fluid, completion, and workover operation protocols is effective in lowering completion costs and increasing production.

  9. 31 CFR 800.218 - Lead agency.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 31 Money and Finance:Treasury 3 2013-07-01 2013-07-01 false Lead agency. 800.218 Section 800.218... FOREIGN PERSONS Definitions § 800.218 Lead agency. The term lead agency means an agency designated by the... activity for which the Chairperson designates it as a lead agency, including all or a portion of a...

  10. 31 CFR 800.218 - Lead agency.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Lead agency. 800.218 Section 800.218... FOREIGN PERSONS Definitions § 800.218 Lead agency. The term lead agency means an agency designated by the... activity for which the Chairperson designates it as a lead agency, including all or a portion of a...

  11. 31 CFR 800.218 - Lead agency.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 31 Money and Finance:Treasury 3 2014-07-01 2014-07-01 false Lead agency. 800.218 Section 800.218... FOREIGN PERSONS Definitions § 800.218 Lead agency. The term lead agency means an agency designated by the... activity for which the Chairperson designates it as a lead agency, including all or a portion of a...

  12. 31 CFR 800.218 - Lead agency.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 31 Money and Finance:Treasury 3 2012-07-01 2012-07-01 false Lead agency. 800.218 Section 800.218... FOREIGN PERSONS Definitions § 800.218 Lead agency. The term lead agency means an agency designated by the... activity for which the Chairperson designates it as a lead agency, including all or a portion of a...

  13. 31 CFR 800.218 - Lead agency.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 31 Money and Finance:Treasury 3 2011-07-01 2011-07-01 false Lead agency. 800.218 Section 800.218... FOREIGN PERSONS Definitions § 800.218 Lead agency. The term lead agency means an agency designated by the... activity for which the Chairperson designates it as a lead agency, including all or a portion of a...

  14. Extending Iterative Protein Redesign and Optimization (IPRO) in Protein Library Design for Ligand Specificity

    PubMed Central

    Fazelinia, Hossein; Cirino, Patrick C.; Maranas, Costas D.

    2007-01-01

    In this article we extend the Iterative Protein Redesign and Optimization (IPRO) framework for the design of protein libraries with targeted ligand specificity. Mutations that minimize the binding energy with the desired ligand are identified. At the same time explicit constraints are introduced that maintain the binding energy for all decoy ligands above a threshold necessary for successful binding. The proposed framework is demonstrated by computationally altering the effector binding specificity of the bacterial transcriptional regulatory protein AraC, belonging to the AraC/XylS family of transcriptional regulators for different unnatural ligands. The obtained results demonstrate the importance of systematically suppressing the binding energy for competing ligands. Pinpointing a small set of mutations within the binding pocket greatly improves the difference in binding energies between targeted and decoy ligands, even when they are very similar. PMID:17208966

  15. Design and specification of a centralized manufacturing data management and scheduling system

    NASA Technical Reports Server (NTRS)

    Farrington, Phillip A.

    1993-01-01

    As was revealed in a previous study, the Materials and Processes Laboratory's Productivity Enhancement Complex (PEC) has a number of automated production areas/cells that are not effectively integrated, limiting the ability of users to readily share data. The recent decision to utilize the PEC for the fabrication of flight hardware has focused new attention on the problem and brought to light the need for an integrated data management and scheduling system. This report addresses this need by developing preliminary designs specifications for a centralized manufacturing data management and scheduling system for managing flight hardware fabrication in the PEC. This prototype system will be developed under the auspices of the Integrated Engineering Environment (IEE) Oversight team and the IEE Committee. At their recommendation the system specifications were based on the fabrication requirements of the AXAF-S Optical Bench.

  16. Optimization of affinity, specificity and function of designed influenza inhibitors using deep sequencing

    SciTech Connect

    Whitehead, Timothy A.; Chevalier, Aaron; Song, Yifan; Dreyfus, Cyrille; Fleishman, Sarel J.; De Mattos, Cecilia; Myers, Chris A.; Kamisetty, Hetunandan; Blair, Patrick; Wilson, Ian A.; Baker, David

    2012-06-19

    We show that comprehensive sequence-function maps obtained by deep sequencing can be used to reprogram interaction specificity and to leapfrog over bottlenecks in affinity maturation by combining many individually small contributions not detectable in conventional approaches. We use this approach to optimize two computationally designed inhibitors against H1N1 influenza hemagglutinin and, in both cases, obtain variants with subnanomolar binding affinity. The most potent of these, a 51-residue protein, is broadly cross-reactive against all influenza group 1 hemagglutinins, including human H2, and neutralizes H1N1 viruses with a potency that rivals that of several human monoclonal antibodies, demonstrating that computational design followed by comprehensive energy landscape mapping can generate proteins with potential therapeutic utility.

  17. The design of a Phase I non site-specific Centralized Interim Storage Facility

    SciTech Connect

    Stringer, J.; Kane, D.

    1997-10-28

    The Department of Energy (DOE), Office of Civilian Radioactive Waste Management (OCRWM) recently completed a Topical Safety Analysis Report (TSAR) for a Phase 1 non site specific Centralized Interim Storage Facility (CISF). The TSAR will be used in licensing the CISF when and if a site is designated. The combined Phase 1 and Phase 2 CISF will provide federal storage capability for 40,000 metric tons of uranium (MTU) Spent Nuclear Fuel (SNF) under the oversight of the DOE. The Phase 1 TSAR was submitted to the NRC on May 1, 1997 and is currently under review having been docketed on June 10, 1997. This paper generally describes the Phase 1 CISF design and its operations as presented in the CISF TSAR.

  18. Design of PI controllers for achieving time and frequency domain specifications simultaneously.

    PubMed

    Hamamci, Serdar Ethem; Tan, Nusret

    2006-10-01

    This paper deals with the design of PI controllers which achieve the desired frequency and time domain specifications simultaneously. A systematic method, which is effective and simple to apply, is proposed. The required values of the frequency domain performance measures namely the gain and phase margins and the time domain performance measures such as settling time and overshoot are defined prior to the design. Then, to meet these desired performance values, a method which presents a graphical relation between the required performance values and the parameters of the PI controller is given. Thus, a set of PI controllers which attain desired performances can be found using the graphical relations. Illustrative examples are given to demonstrate the benefits of the method presented.

  19. Design and synthesis of hairpin probe for specific mis-match discrimination.

    PubMed

    Misra, Arvind; Kumar, Pradeep; Gupta, K C

    2007-01-01

    A single stranded hairpin probe labeled with fluorescein at its 5'-end and terminates with deoxyguanosine nucleotide at 3'- end, as quencher, has been designed and synthesized in an automated DNA synthesizer. The system has been used as an alternative to molecular beacon. The deoxyguanosine residues have been kept at the 3'-end of the complementary arm strand to quench the fluorescence intensity of the fluorophore, making the hairpin probe behave like a conventional molecular beacon. The proposed probe has been used to find a correlation between fluorescence and thermal behaviour on hybridizing it with several mismatched target oligonucleotides. The designed probe has shown greater degree of specificity with perfectly matched target oligonucleotide, while it shows a variable degree of destabilization with mismatched (A/C) target complementary oligonucleotides.

  20. Acoustic specifications for the design of jet engine test facilities on an airbase

    SciTech Connect

    Strumpf, F.M.

    1982-01-01

    The use of engine run up test arrangements was common in Israeli air-bases since the forties, when engines for the Mustang, Mosquito, Harward and other propellor powered planes were used. The era of jet engine propulsion boosted the noise levels, and the use of fighters with afterburners in the new engines of the 80's brought it up to unbearable levels. Thus, the growth of the Israeli Air Force demanded the use of efficient noise suppression devices. These were divided into engine run-up noise suppressors, and aircraft noise suppessors (Hush Houses). For both of the bove ground arrangements, acoustic specifications had to be given. They were, as well as design goals for the manufacturers, also needed to restrict noise levels on the air-base as well as its surroundings. The acoustic specifications discussed are based on measured data, and permitted noise levels in the homes on the base being as far as 2500 meters from the engine exhaust silencer. For the special air-base discussed, various criteria were tested, including US Military Specifications, none of which were acceptable, and a special specification was therefore prepared.

  1. Application of Fraunhofer diffraction theory to feature-specific detector design.

    PubMed

    Hardy, J A; Wheeless, L L

    1977-07-01

    Light scatter from epithelial cells in a slit-scan flow system is modeled using the Fraunhofer condition of scalar diffraction theory. Power spectra are calculated for successive positions of model cells in the line focus of a laser beam with a Fourier transform computer program. Using the calculated power spectra, detector configurations are designed to detect specific cell structures of interest. Detector configurations are tested in a static slit-scan scatter apparatus. Data indicating the ability to detect boundaries and cell orientation are discussed.

  2. Mod-5A wind turbine generator program design report. Volume 4: Drawings and specifications, book 2

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The design, development and analysis of the 7.3 MW MOD-5A wind turbine generator is documented. There are four volumes. This volume contains the drawings and specifications that were developed in preparation for building the MOD-5A wind turbine generator. This is the second book of volume four. Some of the items it contains are specs for the emergency shutdown panel, specs for the simulator software, simulator hardware specs, site operator terminal requirements, control data system requirements, software project management plan, elastomeric teeter bearing requirement specs, specs for the controls electronic cabinet, and specs for bolt pretensioning.

  3. Target design considerations for high specific activity [{sup 11}C]O{sub 2}

    SciTech Connect

    Ferrieri, R.A.; Alexoff, D.L.; Schlyer, D.J.; McDonald, K.; Wolf, A.P.

    1993-12-31

    In the routine preparation of {sup 11}C-labeled compounds through N-[{sup 11}C]-methylation using [{sup 11}C]H{sub 3}I, total masses are always higher than synthesis mass contribution, suggesting that the target system contributes carrier carbon to the final product mass. This conclusion prompted this evaluation of target materials and target design for [{sup 11}C]O{sub 2} production. Ultimately, one is faced with the sprospect of compromising between [{sup 11}C]O{sub 2} specific activity and the amount that can be extracted from the target after a reasonable irradiation time.

  4. Design Analysis of a High Temperature Radiator for the Variable Specific Impulse Magnetoplasma Rocket (VASIMR)

    NASA Technical Reports Server (NTRS)

    Sheth, Rubik B.; Ungar, Eugene K.; Chambliss, Joe P.; Cassady, Leonard D.

    2011-01-01

    The Variable Specific Impulse Magnetoplasma Rocket (VASIMR), currently under development by Ad Astra Rocket Company, is a unique propulsion system that can potentially change the way space propulsion is performed. VASIMR's efficiency, when compared to that of a conventional chemical rocket, reduce propellant needed for exploration missions by a factor of 10. Currently plans include flight tests of a 200 kW VASIMR system, titled VF-200, on the International Space Station. The VF-200 will consist of two 100 kW thruster units packaged together in one engine bus. Each thruster unit has a unique heat rejection requirement of about 27 kW over a firing time of 15 minutes. In order to control rocket core temperatures, peak operating temperatures of about 300 C are expected within the thermal control loop. Design of a high temperature radiator is a unique challenge for the vehicle design. This paper will discuss the path taken to develop a steady state and transient based radiator design. The paper will describe radiator design options for the VASIMR thermal control system for use on ISS as well as future exploration vehicles.

  5. Design of a High Temperature Radiator for the Variable Specific Impulse Magnetoplasma Rocket

    NASA Technical Reports Server (NTRS)

    Sheth, Rubik B.; Ungar, Eugene K.; Chambliss, Joe P.

    2012-01-01

    The Variable Specific Impulse Magnetoplasma Rocket (VASIMR), currently under development by Ad Astra Rocket Company (Webster, TX), is a unique propulsion system that could change the way space propulsion is performed. VASIMR's efficiency, when compared to that of a conventional chemical rocket, reduces the propellant needed for exploration missions by a factor of 10. Currently plans include flight tests of a 200 kW VASIMR system, titled VF-200, on the International Space Station (ISS). The VF-200 will consist of two 100 kW thruster units packaged together in one engine bus. Each thruster core generates 27 kW of waste heat during its 15 minute firing time. The rocket core will be maintained between 283 and 573 K by a pumped thermal control loop. The design of a high temperature radiator is a unique challenge for the vehicle design. This paper will discuss the path taken to develop a steady state and transient-based radiator design. The paper will describe the radiator design option selected for the VASIMR thermal control system for use on ISS, and how the system relates to future exploration vehicles.

  6. 48 CFR 52.246-19 - Warranty of Systems and Equipment under Performance Specifications or Design Criteria.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Equipment under Performance Specifications or Design Criteria. 52.246-19 Section 52.246-19 Federal... under Performance Specifications or Design Criteria. As prescribed in 46.710(c)(1), the contracting officer may insert a clause substantially as follows: Warranty of Systems and Equipment Under...

  7. Multivalent drug design and inhibition of cholera toxin by specific and transient protein-ligand interactions.

    PubMed

    Liu, Jiyun; Begley, Darren; Mitchell, Daniel D; Verlinde, Christophe L M J; Varani, Gabriele; Fan, Erkang

    2008-05-01

    Multivalent inhibitors of the cholera toxin B pentamer are potential therapeutic drugs for treating cholera and serve as models for demonstrating multivalent ligand effects through a structure-based approach. A crucial yet often overlooked aspect of multivalent drug design is the length, rigidity and chemical composition of the linker used to connect multiple binding moieties. To specifically study the role of chemical linkers in multivalent ligand design, we have synthesized a series of compounds with one and two binding motifs connected by several different linkers. These compounds have affinity for and potency against the cholera toxin B pentamer despite the fact that none can simultaneously bind two toxin receptor sites. Results from saturation transfer difference NMR reveal transient, non-specific interactions between the cholera toxin and linker groups contribute significantly to overall binding affinity of monovalent compounds. However, the same random protein-ligand interactions do not appear to affect binding of bivalent molecules. Moreover, the binding affinities and potencies of these 'non-spanning' bivalent ligands appear to be wholly independent of linker length. Our detailed analysis identifies multiple effects that account for the improved inhibitory potencies of bivalent ligands and suggest approaches to further improve the activity of this class of compounds.

  8. A Symmetrical, Planar SOFC Design for NASA's High Specific Power Density Requirements

    NASA Technical Reports Server (NTRS)

    Cable, Thomas L.; Sofie, Stephen W.

    2007-01-01

    Solid oxide fuel cell (SOFC) systems for aircraft applications require an order of magnitude increase in specific power density (1.0 kW/kg) and long life. While significant research is underway to develop anode supported cells which operate at temperatures in the range of 650-800 C, concerns about Cr-contamination from the metal interconnect may drive the operating temperature down further, to 750 C and lower. Higher temperatures, 900-1000 C, are more favorable for SOFC stacks to achieve specific power densities of 1.0 kW/kg. Since metal interconnects are not practical at these high temperatures and can account for up to 75% of the weight of the stack, NASA is pursuing a design that uses a thin, LaCrO3-based ceramic interconnect that incorporates gas channels into the electrodes. The bi-electrode supported cell (BSC) uses porous YSZ scaffolds, on either side of a 10-20 microns electrolyte. The porous support regions are fabricated with graded porosity using the freeze-tape casting process which can be tailored for fuel and air flow. Removing gas channels from the interconnect simplifies the stack design and allows the ceramic interconnect to be kept thin, on the order of 50 -100 microns. The YSZ electrode scaffolds are infiltrated with active electrode materials following the high temperature sintering step. The NASA-BSC is symmetrical and CTE matched, providing balanced stresses and favorable mechanical properties for vibration and thermal cycling.

  9. Power Supply System for the Atlas Experiment: Design Specifications, Implementation, Test and First Results

    NASA Astrophysics Data System (ADS)

    Lazzaroni, M.; Citterio, M.; Latorre, S.; Lanza, A.; Cova, P.; Delmonte, N.; Giuliani, F.

    2014-06-01

    The planned upgrade of instrumentation sensitivity in the ATLAS experiment of the Large Hadron Collider (LHC), at CERN, calls for a new type of power distribution architecture. Moreover, power supplies require DC-DC power converters able to work in very hostile environment and maintaining high level of Reliability, Availability, Maintainability and Safety (denoted as RAMS requirements) during the experimental activity. Two main issues need to be discussed: first, electronic devices and equipment must operate in very high background of both charged and neutral particles and high static magnetic field and, second, the increase of the radiation background and the requirements of new front-end electronics are indeed incompatible with the current capability of the actual distribution system. The APOLLO R&D collaboration, funded by the Italian Istituto Nazionale di Fisica Nucleare (INFN), aims to study dedicated topologies of both distribution system and DC-DC power converters and to design, build and test demonstrators, developing the needed technology for the industrialization phase. The collaboration has designed a 3kW, 280V-12V converter (MC) based on the Switch in Line architecture (SIL), a DC to DC phase-shifted converter characterized by a disposition in line of the MOSFETs with good soft switching performances, and in the last year many steps have been taken to enhance the power dissipation and the reliability and to improve the general features of the designed converter. In particular a new water heat sink was designed on the basis of TFD simulation accounting for the layout of the specific converter. Experimental activities in order to characterize both thermal and electrical features of the MC confirm the correctness of the adopted design criteria.

  10. Exploring alternate specifications to explain agency-level effects in placement decisions regarding Aboriginal children: Further analysis of the Canadian Incidence Study of Reported Child Abuse and Neglect Part C.

    PubMed

    Fallon, Barbara; Chabot, Martin; Fluke, John; Blackstock, Cindy; Sinha, Vandna; Allan, Kate; MacLaurin, Bruce

    2015-11-01

    A series of papers using data from the Canadian Incidence Study of Reported Child Abuse and Neglect (CIS) explored the influence of clinical and organizational characteristics on the decision to place Aboriginal children in out-of-home placements at the conclusion of child maltreatment investigations. The purpose of this paper is to further explore a consistent finding of the previous analyses: the proportion of investigations involving Aboriginal children at a child welfare agency is associated with placement for all children in that agency. CIS-2008 data were used in the analysis, which allowed for inclusion of previously unavailable organizational and contextual variables. Multi-level statistical models were developed to analyze the influence of clinical and organizational variables on the placement decision. Final models revealed that the proportion of investigations conducted by the child welfare agency involving Aboriginal children was again a key agency-level predictor of the placement decision for any child served by the agency. Specifically, the higher the proportion of investigations of Aboriginal children, the more likely placement was to occur for any child. Further, this analysis demonstrated that structure of governance, an organizational-level variable not available in previous cycles of the CIS, is an important agency-level predictor of out-of-home placement. Further analysis is needed to fully understand individual and organizational level variables that may influence decisions regarding placement of Aboriginal children.

  11. Exploring alternate specifications to explain agency-level effects in placement decisions regarding Aboriginal children: Further analysis of the Canadian Incidence Study of Reported Child Abuse and Neglect Part C.

    PubMed

    Fallon, Barbara; Chabot, Martin; Fluke, John; Blackstock, Cindy; Sinha, Vandna; Allan, Kate; MacLaurin, Bruce

    2015-11-01

    A series of papers using data from the Canadian Incidence Study of Reported Child Abuse and Neglect (CIS) explored the influence of clinical and organizational characteristics on the decision to place Aboriginal children in out-of-home placements at the conclusion of child maltreatment investigations. The purpose of this paper is to further explore a consistent finding of the previous analyses: the proportion of investigations involving Aboriginal children at a child welfare agency is associated with placement for all children in that agency. CIS-2008 data were used in the analysis, which allowed for inclusion of previously unavailable organizational and contextual variables. Multi-level statistical models were developed to analyze the influence of clinical and organizational variables on the placement decision. Final models revealed that the proportion of investigations conducted by the child welfare agency involving Aboriginal children was again a key agency-level predictor of the placement decision for any child served by the agency. Specifically, the higher the proportion of investigations of Aboriginal children, the more likely placement was to occur for any child. Further, this analysis demonstrated that structure of governance, an organizational-level variable not available in previous cycles of the CIS, is an important agency-level predictor of out-of-home placement. Further analysis is needed to fully understand individual and organizational level variables that may influence decisions regarding placement of Aboriginal children. PMID:25943285

  12. Design and evaluation of matrices of Eudragit with polycarbophil and carbopol for colon-specific delivery.

    PubMed

    Asghar, Laila Fatima Ali; Chandran, Sajeev

    2008-12-01

    The purpose of the present study was to investigate the effect of incorporating pH-responsive polymers Eudragit (L100 or S100) in matrix bases composed of hydrophilic polymers polycarbophil and carbopol to design oral controlled release formulations with sigmoidal release profile for colon-specific delivery. Matrix tablets were prepared by wet granulation technique using indomethacin as model drug and were characterized for physical parameters, in vitro drug release, release kinetics, and stability on storage. The gastrointestinal (GI) transit of selected formulations was also investigated in human subjects using gamma scintigraphy. In vitro release studies indicated that the presence of pH-sensitive polymers in hydrophilic polymer base retarded the initial release significantly (10-15% release in 6 h) followed with controlled release for the next 8-10 h in simulated GI fluid pH (without enzymes). The presence of Eudragit in hydrophilic matrix base retarded the swelling of the matrix base in acidic to weakly acidic pH, but in alkaline pH, enhancement in drug release rate was observed due to the dissolution of Eudragit from the base resulting in a porous matrix structure, resulting in around 80-90% release in 14 h of study. In vivo gamma scintigraphy studies in healthy human subjects proved that the formulations had acceptable matrix strength to withstand gastric and colonic transit. The mean colonic residence time of selected designed formulations varied between 15 and 19 h. Such a matrix design could have potential application as colon-specific drug delivery systems with pH- and time-dependent drug release profile.

  13. The Effects of Goal Specificity and Scaffolding on Programming Performance and Self-Regulation in Game Design

    ERIC Educational Resources Information Center

    Feng, Chia-Yen; Chen, Ming-Puu

    2014-01-01

    The purpose of this study was to investigate the influence of goal specificity and scaffolding on the programming performance and self-regulation of elementary students engaged in learning game design. This study recruited 232 students for the experimental activities. Two levels of goal specificity were employed: specific and nonspecific.…

  14. NDA PDP Program PuO{sub 2} increased particle size specification and design

    SciTech Connect

    Marshall, R.S.; Taggart, D.P.; Becker, G.K.; Woon, W.Y.

    1996-12-31

    Provisions in the National TRU Program Quality Assurance Program Plan require an assessment of performance for nondestructive waste assay (NDA) systems employed in the program. This requirement is in part fulfilled through the use of Performance Demonstration programs. In order to optimize the quality and quantity of information acquired during a given Performance Demonstration Program cycle, the assessment employed is to be carefully specified and designed. The assessment must yield measurement system performance data meaningful with respect to NDA system capability to accommodate attributes of interest known to occur in actual waste forms. The design and specification of the increased particle size PuO{sub 2} PDP working reference materials (WRMs) is directed at providing a straightforward mechanism to assess waste NDA system capability to account for biases introduced by large PuO{sub 2} particles. The increased particle size PuO{sub 2} PDP WRM design addresses actual waste form attributes associated with PuO{sub 2} particle size and distributions thereof, the issue of a known and stable WRM configuration and equally important appropriate certification and tractability considerations.

  15. The Effect of Femoral Cutting Guide Design Improvements for Patient-Specific Instruments

    PubMed Central

    Kwon, Oh-Ryong; Kang, Kyoung-Tak; Son, Juhyun; Choi, Yun-Jin; Suh, Dong-Suk; Koh, Yong-Gon

    2015-01-01

    Although the application of patient-specific instruments (PSI) for total knee arthroplasty (TKA) increases the cost of the surgical procedure, PSI may reduce operative time and improve implant alignment, which could reduce the number of revision surgeries. We report our experience with TKA using PSI techniques in 120 patients from March to December 2014. PSI for TKA were created from data provided by computed tomography (CT) scans or magnetic resonance imaging (MRI); which imaging technology is more reliable for the PSI technique remains unclear. In the first 20 patients, the accuracy of bone resection and PSI stability were compared between CT and MRI scans with presurgical results as a reference; MRI produced better results. In the second and third groups, each with 50 patients, the results of bone resection and stability were compared in MRI scans with respect to the quality of scanning due to motion artifacts and experienced know-how in PSI design, respectively. The optimized femoral cutting guide design for PSI showed the closest outcomes in bone resection and PSI stability with presurgical data. It is expected that this design could be a reasonable guideline in PSI. PMID:26881210

  16. Earth Observatory Satellite system definition study. Report 5: System design and specifications. Volume 3: General purpose spacecraft segment and module specifications

    NASA Technical Reports Server (NTRS)

    1974-01-01

    The specifications for the Earth Observatory Satellite (EOS) general purpose aircraft segment are presented. The satellite is designed to provide attitude stabilization, electrical power, and a communications data handling subsystem which can support various mission peculiar subsystems. The various specifications considered include the following: (1) structures subsystem, (2) thermal control subsystem, (3) communications and data handling subsystem module, (4) attitude control subsystem module, (5) power subsystem module, and (6) electrical integration subsystem.

  17. Earth Observatory Satellite system definition study. Report 5: System design and specifications. Volume 2: EOS-A system specification

    NASA Technical Reports Server (NTRS)

    1974-01-01

    The objectives of the Earth Observatory Satellite (EOS) program are defined. The system specifications for the satellite payload are examined. The broad objectives of the EOS-A program are as follows: (1) to develop space-borne sensors for the measurement of land resources, (2) to evolve spacecraft systems and subsystems which will permit earth observation with greater accuracy, coverage, spatial resolution, and continuity than existing systems, (3) to develop improved information processing, extraction, display, and distribution systems, and (4) to use space transportation systems for resupply and retrieval of the EOS.

  18. The Effects on Children of the Organization and the Design of the Day Care Physical Environment: Appropriateness of the Federal Inter-Agency Day Care Requirements. Final Report.

    ERIC Educational Resources Information Center

    Kruvant, Charito; And Others

    This paper presents a discussion of the impact of the physical design of day care settings on children and adults. Specifically addressed is the question of how the location and design of the facility, the arrangement of indoor and outdoor space, and the organization of the furniture and materials affect the behavior of the adults and children who…

  19. Signal processing and display interface studies. [performance tests - design analysis/equipment specifications

    NASA Technical Reports Server (NTRS)

    1975-01-01

    Signal processing equipment specifications, operating and test procedures, and systems design and engineering are described. Five subdivisions of the overall circuitry are treated: (1) the spectrum analyzer; (2) the spectrum integrator; (3) the velocity discriminator; (4) the display interface; and (5) the formatter. They function in series: (1) first in analog form to provide frequency resolution, (2) then in digital form to achieve signal to noise improvement (video integration) and frequency discrimination, and (3) finally in analog form again for the purpose of real-time display of the significant velocity data. The formatter collects binary data from various points in the processor and provides a serial output for bi-phase recording. Block diagrams are used to illustrate the system.

  20. Detailed design specification for a prototype Assessment System for Aircraft Noise (ASAN)

    NASA Astrophysics Data System (ADS)

    Sanford, Fidell; Harris, Michael; Reddinguis, Nicholaas

    1988-07-01

    The U.S. Air Force Noise and Sonic Boom Impact Technology (NSBIT) Program is sponsoring a multi-stage effort to create a computer system containing tools needed by the environmental planning community to perform a variety of tasks related to assessing the environmental impacts of aircraft noise on people, animals, and structures. This interim report provides a detailed design specification for a prototype version of the NSBIT Assessment System for Aircraft Noise (ASAN) that is the major project of the first stage of this effort. The purposes and expected uses of ASAN are presented in Fidell and Harris (1987). The general functional capabilities of this system are described by Harris and Fidell (1987). The current report describes the organization of ASAN, its functional capabilities, and its major software modules.

  1. Subcontract Report: Modular Combined Heat & Power System for Utica College: Design Specification

    SciTech Connect

    Rouse, Greg

    2007-09-01

    Utica College, located in Utica New York, intends to install an on-site power/cogeneration facility. The energy facility is to be factory pre-assembled, or pre- assembled in modules, to the fullest extent possible, and ready to install and interconnect at the College with minimal time and engineering needs. External connections will be limited to fuel supply, electrical output, potable makeup water as required and cooling and heat recovery systems. The proposed facility will consist of 4 self-contained, modular Cummins 330kW engine generators with heat recovery systems and the only external connections will be fuel supply, electrical outputs and cooling and heat recovery systems. This project was eventually cancelled due to changing DOE budget priorities, but the project engineers produced this system design specification in hopes that it may be useful in future endeavors.

  2. Vortex design of a diagonal flow impeller with high specific speed

    NASA Astrophysics Data System (ADS)

    Kamada, Yoshihisa; Yamaguchi, Sumio; Sasaki, Kazuto; Inoue, Masahiro

    1986-09-01

    The 'vortex design' method applied to an axial flow compressor stage has been extended to a diagonal flow impeller with high specific speed. For a given type of vortex flow, the through flow problem is solved by the streamline curvature method, and a blade element is determined on the basis of this solution. However, for any vortex type except free vortex, the exit flow condition changes due to a secondary flow induced by vortices shed from the trailing edges. The given vortex type can be obtained by correcting this effect with a so-called secondary flow theory.The validity of this method has been examined in experiments on three kinds of vortex flows: free vortex type, and constant tangential velocity types with or without correction of the secondary flow effects.

  3. Effectiveness of an Applied Microbiology Course Specifically Designed for Chemical Engineering Majors

    PubMed Central

    HECHT, GREGORY B.; MOSTO, PATRICIA; SLATER, C. STEWART

    2003-01-01

    In recent years, the disciplines of microbiology and chemical engineering have developed an increasing convergence. To meet the needs of their future employers, today’s chemical engineering students must receive some background in microbiology. This report describes the development and content of “Biological Systems and Applications,” a novel course specifically designed to provide basic biology and applied microbiology knowledge, skills, and experience to sophomore chemical engineering majors. Data collected from entrance and exit surveys of the students demonstrated that the course is successful. The importance of the “project-base” learning technique and of interdisciplinary faculty-student and faculty-faculty collaborations are proposed as elements essential to the success of this particular course. PMID:23653549

  4. Detailed design specification for the ALT Shuttle Information Extraction Subsystem (SIES)

    NASA Technical Reports Server (NTRS)

    Clouette, G. L.; Fitzpatrick, W. N.

    1976-01-01

    The approach and landing test (ALT) shuttle information extraction system (SIES) is described in terms of general requirements and system characteristics output products and processing options, output products and data sources, and system data flow. The ALT SIES is a data reduction system designed to satisfy certain data processing requirements for the ALT phase of the space shuttle program. The specific ALT SIES data processing requirements are stated in the data reduction complex approach and landing test data processing requirements. In general, ALT SIES must produce time correlated data products as a result of standardized data reduction or special purpose analytical processes. The main characteristics of ALT SIES are: (1) the system operates in a batch (non-interactive) mode; (2) the processing is table driven; (3) it is data base oriented; (4) it has simple operating procedures; and (5) it requires a minimum of run time information.

  5. Probing the Surface of Human Carbonic Anhydrase for Clues towards the Design of Isoform Specific Inhibitors

    PubMed Central

    Pinard, Melissa A.

    2015-01-01

    The alpha carbonic anhydrases (α-CAs) are a group of structurally related zinc metalloenzymes that catalyze the reversible hydration of CO2 to HCO3−. Humans have 15 different α-CAs with numerous physiological roles and expression patterns. Of these, 12 are catalytically active, and abnormal expression and activities are linked with various diseases, including glaucoma and cancer. Hence there is a need for CA isoform specific inhibitors to avoid off-target CA inhibition, but due to the high amino acid conservation of the active site and surrounding regions between each enzyme, this has proven difficult. However, residues towards the exit of the active site are variable and can be exploited to design isoform selective inhibitors. Here we discuss and characterize this region of “selective drug targetability” and how these observations can be utilized to develop isoform selective CA inhibitors. PMID:25811028

  6. A multi-agency sutrient dataset used to estimate loads, improve monitoring design, and calibrate regional sutrient SPARROW models

    USGS Publications Warehouse

    Saad, David A.; Schwarz, Gregory; Robertson, Dale M.; Booth, Nathaniel

    2011-01-01

    Stream-loading information was compiled from federal, state, and local agencies, and selected universities as part of an effort to develop regional SPAtially Referenced Regressions On Watershed attributes (SPARROW) models to help describe the distribution, sources, and transport of nutrients in streams throughout much of the United States. After screening, 2,739 sites, sampled by 73 agencies, were identified as having suitable data for calculating long-term mean annual nutrient loads required for SPARROW model calibration. These sites had a wide range in nutrient concentrations, loads, and yields, and environmental characteristics in their basins. An analysis of the accuracy in load estimates relative to site attributes indicated that accuracy in loads improve with increases in the number of observations, the proportion of uncensored data, and the variability in flow on observation days, whereas accuracy declines with increases in the root mean square error of the water-quality model, the flow-bias ratio, the number of days between samples, the variability in daily streamflow for the prediction period, and if the load estimate has been detrended. Based on compiled data, all areas of the country had recent declines in the number of sites with sufficient water-quality data to compute accurate annual loads and support regional modeling analyses. These declines were caused by decreases in the number of sites being sampled and data not being entered in readily accessible databases.

  7. 34 CFR 370.12 - How does a designated agency preserve its right to appeal a redesignation?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Education (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION CLIENT ASSISTANCE PROGRAM What Requirements Apply to Redesignation? § 370.12 How does a designated...

  8. 34 CFR 370.12 - How does a designated agency preserve its right to appeal a redesignation?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Education (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION CLIENT ASSISTANCE PROGRAM What Requirements Apply to Redesignation? § 370.12 How does a designated...

  9. Design of Internalizing PSMA-specific Glu-ureido-based Radiotherapeuticals.

    PubMed

    Wüstemann, Till; Bauder-Wüst, Ulrike; Schäfer, Martin; Eder, Matthias; Benesova, Martina; Leotta, Karin; Kratochwil, Clemens; Haberkorn, Uwe; Kopka, Klaus; Mier, Walter

    2016-01-01

    Despite the progress in diagnosis and treatment, prostate cancer (PCa) is one of the main causes for cancer-associated deaths among men. Recently, prostate-specific membrane antigen (PSMA) binding tracers have revolutionized the molecular imaging of this disease. The translation of these tracers into therapeutic applications is challenging because of high PSMA-associated kidney uptake. While both the tumor uptake and the uptake in the kidneys are PSMA-specific, the kidneys show a more rapid clearance than tumor lesions. Consequently, the potential of endoradiotherapeutic drugs targeting PSMA is highly dependent on a sustained retention in the tumor - ideally achieved by predominant internalization of the respective tracer. Previously, we were able to show that the pharmacokinetics of the tracers containing the Glu-urea-based binding motif can be further enhanced with a specifically designed linker. Here, we evaluate an eventual influence of the chelator moiety on the pharmacokinetics, including the tumor internalization. A series of tracers modified by different chelators were synthesized using solid phase chemistry. The conjugates were radiolabeled to evaluate the influence on the receptor binding affinity, the ligand-induced internalization and the biodistribution behavior. Competitive binding and internalization assays were performed on PSMA positive LNCaP cells and the biodistribution of the most promising compound was evaluated by positron emission tomography (PET) in LNCaP-tumor-bearing mice. Interestingly, conjugation of the different chelators did not cause significant differences: all compounds showed nanomolar binding affinities with only minor differences. PET imaging of the (68)Ga-labeled CHX-A''-DTPA conjugate revealed that the chelator moiety does not impair the specificity of tumor uptake when compared to the gold standard PSMA-617. However, strong differences of the internalization ratios caused by the chelator moiety were observed: differences in

  10. Design of Internalizing PSMA-specific Glu-ureido-based Radiotherapeuticals

    PubMed Central

    Wüstemann, Till; Bauder-Wüst, Ulrike; Schäfer, Martin; Eder, Matthias; Benesova, Martina; Leotta, Karin; Kratochwil, Clemens; Haberkorn, Uwe; Kopka, Klaus; Mier, Walter

    2016-01-01

    Despite the progress in diagnosis and treatment, prostate cancer (PCa) is one of the main causes for cancer-associated deaths among men. Recently, prostate-specific membrane antigen (PSMA) binding tracers have revolutionized the molecular imaging of this disease. The translation of these tracers into therapeutic applications is challenging because of high PSMA-associated kidney uptake. While both the tumor uptake and the uptake in the kidneys are PSMA-specific, the kidneys show a more rapid clearance than tumor lesions. Consequently, the potential of endoradiotherapeutic drugs targeting PSMA is highly dependent on a sustained retention in the tumor - ideally achieved by predominant internalization of the respective tracer. Previously, we were able to show that the pharmacokinetics of the tracers containing the Glu-urea-based binding motif can be further enhanced with a specifically designed linker. Here, we evaluate an eventual influence of the chelator moiety on the pharmacokinetics, including the tumor internalization. A series of tracers modified by different chelators were synthesized using solid phase chemistry. The conjugates were radiolabeled to evaluate the influence on the receptor binding affinity, the ligand-induced internalization and the biodistribution behavior. Competitive binding and internalization assays were performed on PSMA positive LNCaP cells and the biodistribution of the most promising compound was evaluated by positron emission tomography (PET) in LNCaP-tumor-bearing mice. Interestingly, conjugation of the different chelators did not cause significant differences: all compounds showed nanomolar binding affinities with only minor differences. PET imaging of the 68Ga-labeled CHX-A''-DTPA conjugate revealed that the chelator moiety does not impair the specificity of tumor uptake when compared to the gold standard PSMA-617. However, strong differences of the internalization ratios caused by the chelator moiety were observed: differences in

  11. Relaxing the coenzyme specificity of 1,3-propanediol oxidoreductase from Klebsiella pneumoniae by rational design.

    PubMed

    Ma, Chengwei; Zhang, Le; Dai, Jianying; Xiu, Zhilong

    2010-04-15

    1,3-Propanediol has wide applications for large volume markets, particularly in the polymer business. Microbial production of 1,3-propanediol has been considered as a competitor to the traditional petrochemical routes. However, the formation of 1,3-propanediol is limited by the amount of NADH supplied by the oxidative pathway of glycerol dismutation. Previous metabolic flux analysis revealed that relaxation of the coenzyme specificity of 1,3-propanediol oxidoreductase for both NADH and NADPH would increase the production of 1,3-propanediol as well as maintaining the NADH-NAD(+) circle. This work tried to accomplish such a relaxation by rational protein design. Overall binding free energy indicated that the electrostatic energy was the major force discriminating NADH from NADPH. Computational alanine-scanning mutagenesis of the active site residues illustrated that Asp41 was the key residue responsible for the coenzyme specificity. Compared with Asp41Ala, Asp41Gly could further weaken the repulsion between Asp41 and the phosphate group esterified to the 2'-hydroxyl group of the ribose at the adenine end of NADPH. Site-directed mutagenesis was conducted and the relaxation was successfully realized.

  12. Design and screening of a chimeric survivin-specific nanobody and its anticancer activities in vitro.

    PubMed

    Zhang, Na; Guo, Hua; Zheng, Wenyun; Wang, Tianwen; Ma, Xingyuan

    2016-10-01

    Survivin is a strong inhibitor of apoptosis protein and a promising target for cancer prevention and treatment. Here, we report the design and preparation of novel chimeric nanobodies (Nbs) that could specifically bind to survivin. We screened the peptides from phage-displayed libraries (7-mer, 12-mer) for nonconserved sequences of complementarity-determining regions (CDRs) in the scaffold of the Nb. By a combination of the nonconserved sequences for CDRs, the corresponding chimeric Nbs (10 Nbs) were prepared with genetic operations. The antisurvivin Nb TAT-Nb4A (a fusion with cellular transduction peptide TAT) was found to be the most efficient antibody on the basis of the results from enzyme-linked immunosorbent assay, MTT, and flow cytometry when these nanobodies were tested with hepatoma carcinoma cell HepG2. TAT-Nb4A could inhibit the growth of HepG2 and promote cancer cell apoptosis significantly in a dose-dependent and time-dependent manner: the apoptosis rate reached 52.5% when the concentration of TAT-Nb4A was 120 μg/ml. Western blotting with cells expressing survivin showed that the prepared nanobody could efficiently bind to expressed survivin and blocked the signaling pathway in which survivin played a role. This study provided a convenient and feasible method of obtaining a novel specific Nb with the case of survivin as a good example. PMID:27362789

  13. Design of compact freeform lens for application specific Light-Emitting Diode packaging.

    PubMed

    Wang, Kai; Chen, Fei; Liu, Zongyuan; Luo, Xiaobing; Liu, Sheng

    2010-01-18

    Application specific LED packaging (ASLP) is an emerging technology for high performance LED lighting. We introduced a practical design method of compact freeform lens for extended sources used in ASLP. A new ASLP for road lighting was successfully obtained by integrating a polycarbonate compact freeform lens of small form factor with traditional LED packaging. Optical performance of the ASLP was investigated by both numerical simulation based on Monte Carlo ray tracing method and experiments. Results demonstrated that, comparing with traditional LED module integrated with secondary optics, the ASLP had advantages of much smaller size in volume (approximately 1/8), higher system lumen efficiency (approximately 8.1%), lower cost and more convenience for customers to design and assembly, enabling possible much wider applications of LED for general road lighting. Tolerance analyses were also conducted. Installation errors of horizontal and vertical deviations had more effects on the shape and uniformity of radiation pattern compared with rotational deviation. The tolerances of horizontal, vertical and rotational deviations of this lens were 0.11 mm, 0.14 mm and 2.4 degrees respectively, which were acceptable in engineering. PMID:20173861

  14. Design of universal cancer vaccines using natural tumor vessel-specific antigens (SANTAVAC).

    PubMed

    Lokhov, Petr G; Balashova, Elena E

    2015-01-01

    Vaccination against endothelial cells (ECs) lining the tumor vasculature represents one of the most attractive potential cancer immunotherapy options due to its ability to prevent solid tumor growth. Using this approach, target antigens can be derived from ECs and used to develop a universal cancer vaccine. Unfortunately, direct immunization with EC preparations can elicit autoimmune vasculitis in normal tissues. Recently, tumor-induced changes to the human EC surface were described that provided a basis for designing efficient EC-based vaccines capable of eliciting immune responses that targeted the tumor endothelium directly. This review examines these data from the perspective of designing EC-based cancer vaccines for the treatment of all solid tumors, including the antigen composition of vaccine formulations, the selection ECs for antigen derivation, the production and control of antigens, and the method for estimating vaccine efficacy and safety. As the vaccine preparation requires a specifically derived set of natural cell surface antigens, a new vaccine preparation concept was formulated. Antigen compositions prepared according to this concept were named SANTAVAC (Set of All Natural Target Antigens for Vaccination Against Cancer).

  15. Acoustic Characteristics of Various Treatment Panel Designs Specific to HSCT Mixer-Ejector Application

    NASA Technical Reports Server (NTRS)

    Salikuddin, M.; Kinzie, K.; Vu, D. D.; Langenbrunner, L. E.; Szczepkowski, G. T.

    2006-01-01

    The development process of liner design methodology is described in several reports. The results of the initial effort of concept development, screening, laboratory testing of various liner concepts, and preliminary correlation (generic data) are presented in a report Acoustic Characteristics of Various Treatment Panel Designs for HSCT Ejector Liner Acoustic Technology Development Program. The second phase of laboratory test results of more practical concepts and their data correlations are presented in this report (product specific). In particular, this report contains normal incidence impedance measurements of several liner types in both a static rig and in a high temperature flow duct rig. The flow duct rig allows for temperatures up to 400 F with a grazing flow up to Mach 0.8. Measurements of impedance, DC flow resistance, and in the flow rig cases, impact of the liner on boundary layer profiles are documented. In addition to liner rig tests, a limited number of tests were made on liners installed in a mixer-Ejector nozzle to confirm the performance of the liner prediction in an installed configuration.

  16. Specific effects in microwave chemistry explored through reactor vessel design, theory, and spectroscopy.

    PubMed

    Ashley, Bridgett; Lovingood, Derek D; Chiu, Yu-Che; Gao, Hanwei; Owens, Jeffery; Strouse, Geoffrey F

    2015-11-01

    Microwave chemistry has revolutionized synthetic methodology for the preparation of organics, pharmaceuticals, materials, and peptides. The enhanced reaction rates commonly observed in a microwave have led to wide speculation about the function of molecular microwave absorption and whether the absorption leads to microwave specific effects and enhanced molecular heating. The comparison of theoretical modeling, reactor vessel design, and dielectric spectroscopy allows the nuance of the interaction to be directly understood. The study clearly shows an unaltered silicon carbide vessel allows measurable microwave penetration and therefore, molecular absorption of the microwave photons by the reactants within the reaction vessel cannot be ignored when discussing the role of molecular heating in enhanced molecular reactivity for microwave synthesis. The results of the study yield an improved microwave reactor vessel design that eliminates microwave leakage into the reaction volume by incorporating a noble metal surface layer onto a silicon carbide reaction vessel. The systematic study provides the necessary theory and measurements to better inform the arguments in the field.

  17. Design of compact freeform lens for application specific Light-Emitting Diode packaging.

    PubMed

    Wang, Kai; Chen, Fei; Liu, Zongyuan; Luo, Xiaobing; Liu, Sheng

    2010-01-18

    Application specific LED packaging (ASLP) is an emerging technology for high performance LED lighting. We introduced a practical design method of compact freeform lens for extended sources used in ASLP. A new ASLP for road lighting was successfully obtained by integrating a polycarbonate compact freeform lens of small form factor with traditional LED packaging. Optical performance of the ASLP was investigated by both numerical simulation based on Monte Carlo ray tracing method and experiments. Results demonstrated that, comparing with traditional LED module integrated with secondary optics, the ASLP had advantages of much smaller size in volume (approximately 1/8), higher system lumen efficiency (approximately 8.1%), lower cost and more convenience for customers to design and assembly, enabling possible much wider applications of LED for general road lighting. Tolerance analyses were also conducted. Installation errors of horizontal and vertical deviations had more effects on the shape and uniformity of radiation pattern compared with rotational deviation. The tolerances of horizontal, vertical and rotational deviations of this lens were 0.11 mm, 0.14 mm and 2.4 degrees respectively, which were acceptable in engineering.

  18. The Johnson Space Center Management Information Systems (JSCMIS). 1: Requirements Definition and Design Specifications for Versions 2.1 and 2.1.1. 2: Documented Test Scenario Environments. 3: Security Design and Specifications

    NASA Technical Reports Server (NTRS)

    1986-01-01

    The Johnson Space Center Management Information System (JSCMIS) is an interface to computer data bases at NASA Johnson which allows an authorized user to browse and retrieve information from a variety of sources with minimum effort. This issue gives requirements definition and design specifications for versions 2.1 and 2.1.1, along with documented test scenario environments, and security object design and specifications.

  19. Rational design and applications of a Rac GTPase-specific small molecule inhibitor.

    PubMed

    Akbar, Huzoor; Cancelas, Jose; Williams, David A; Zheng, Jie; Zheng, Yi

    2006-01-01

    Rac GTPases are involved in the regulation of multiple cell functions and have been implicated in the pathology of certain human diseases. Dominant negative mutants of Rac have been the tool of choice in studying Rac function in cells. Given the difficulty of introducing high concentrations of the Rac mutants into primary cells and nonspecific effects of the mutants on Rho guanine nucleotide exchange factor (GEF) activities, it is desirable to develop small molecule inhibitors that could specifically inhibit Rac activities. Here we describe the rational design, characterization, and applications of a first-generation Rac-specific small molecule inhibitor. On the basis of the structure-function information of Rac interaction with GEFs, in a computer-based virtual screening we have identified NSC23766, a highly soluble and membrane permeable compound, as a specific inhibitor of a subset of GEF binding to Rac and, therefore, Rac activation by these GEFs. In fibroblast cells, NSC23766 inhibited Rac1 GTP-loading without affecting Cdc42 or RhoA activity and suppressed cell proliferation induced by a Rac GEF Tiam1. It has little effect on cell growth induced by a constitutively active Rac1 mutant. In addition, NSC23766 inhibited: (1) the anchorage-independent growth and invasion phenotypes of human prostate cancer PC-3 cells; (2) Rac activation and Rac-dependent aggregation of platelets stimulated by thrombin; and (3) Rac1 and Rac2 activities of hematopoietic stem/progenitor cells and induced their mobilization from mouse bone marrow to peripheral blood. Thus, NSC23766 is a lead small molecule inhibitor of Rac activity and could be useful for studying Rac-mediated cellular functions and for modulating pathological conditions in which Rac-deregulation may play a role.

  20. Biomaterial design for specific cellular interactions: Role of surface functionalization and geometric features

    NASA Astrophysics Data System (ADS)

    Kolhar, Poornima

    The areas of drug delivery and tissue engineering have experienced extraordinary growth in recent years with the application of engineering principles and their potential to support and improve the field of medicine. The tremendous progress in nanotechnology and biotechnology has lead to this explosion of research and development in biomedical applications. Biomaterials can now be engineered at a nanoscale and their specific interactions with the biological tissues can be modulated. Various design parameters are being established and researched for design of drug-delivery carriers and scaffolds to be implanted into humans. Nanoparticles made from versatile biomaterial can deliver both small-molecule drugs and various classes of bio-macromolecules, such as proteins and oligonucleotides. Similarly in the field of tissue engineering, current approaches emphasize nanoscale control of cell behavior by mimicking the natural extracellular matrix (ECM) unlike, traditional scaffolds. Drug delivery and tissue engineering are closely connected fields and both of these applications require materials with exceptional physical, chemical, biological, and biomechanical properties to provide superior therapy. In the current study the surface functionalization and the geometric features of the biomaterials has been explored. In particular, a synthetic surface for culture of human embryonic stem cells has been developed, demonstrating the importance of surface functionalization in maintaining the pluripotency of hESCs. In the second study, the geometric features of the drug delivery carriers are investigated and the polymeric nanoneedles mediated cellular permeabilization and direct cytoplasmic delivery is reported. In the third study, the combined effect of surface functionalization and geometric modification of carriers for vascular targeting is enunciated. These studies illustrate how the biomaterials can be designed to achieve various cellular behaviors and control the

  1. Specification and design of a Therapy Imaging and Model Management System (TIMMS)

    NASA Astrophysics Data System (ADS)

    Lemke, Heinz U.; Berliner, Leonard

    2007-03-01

    Appropriate use of Information and Communication Technology (ICT) and Mechatronic (MT) systems is considered by many experts as a significant contribution to improve workflow and quality of care in the Operating Room (OR). This will require a suitable IT infrastructure as well as communication and interface standards, such as DICOM and suitable extensions, to allow data interchange between surgical system components in the OR. A conceptual design of such an infrastructure, i.e. a Therapy Imaging and Model Management System (TIMMS) will be introduced in this paper. A TIMMS should support the essential functions that enable and advance image, and in particular, patient model guided therapy. Within this concept, the image centric world view of the classical PACS technology is complemented by an IT model-centric world view. Such a view is founded in the special modelling needs of an increasing number of modern surgical interventions as compared to the imaging intensive working mode of diagnostic radiology, for which PACS was originally conceptualised and developed. A proper design of a TIMMS, taking into account modern software engineering principles, such as service oriented architecture, will clarify the right position of interfaces and relevant standards for a Surgical Assist System (SAS) in general and their components specifically. Such a system needs to be designed to provide a highly modular structure. Modules may be defined on different granulation levels. A first list of components (e.g. high and low level modules) comprising engines and repositories of an SAS, which should be integrated by a TIMMS, will be introduced in this paper.

  2. A Multi-Agency Nutrient Dataset Used to Estimate Loads, Improve Monitoring Design, and Calibrate Regional Nutrient SPARROW Models

    USGS Publications Warehouse

    Saad, D.A.; Schwarz, G.E.; Robertson, D.M.; Booth, N.L.

    2011-01-01

    Stream-loading information was compiled from federal, state, and local agencies, and selected universities as part of an effort to develop regional SPAtially Referenced Regressions On Watershed attributes (SPARROW) models to help describe the distribution, sources, and transport of nutrients in streams throughout much of the United States. After screening, 2,739 sites, sampled by 73 agencies, were identified as having suitable data for calculating long-term mean annual nutrient loads required for SPARROW model calibration. These sites had a wide range in nutrient concentrations, loads, and yields, and environmental characteristics in their basins. An analysis of the accuracy in load estimates relative to site attributes indicated that accuracy in loads improve with increases in the number of observations, the proportion of uncensored data, and the variability in flow on observation days, whereas accuracy declines with increases in the root mean square error of the water-quality model, the flow-bias ratio, the number of days between samples, the variability in daily streamflow for the prediction period, and if the load estimate has been detrended. Based on compiled data, all areas of the country had recent declines in the number of sites with sufficient water-quality data to compute accurate annual loads and support regional modeling analyses. These declines were caused by decreases in the number of sites being sampled and data not being entered in readily accessible databases. ?? 2011 American Water Resources Association. This article is a U.S. Government work and is in the public domain in the USA.

  3. A rationally designed tyrosine hydroxylase DNA vaccine induces specific antineuroblastoma immunity.

    PubMed

    Huebener, Nicole; Fest, Stefan; Strandsby, Anne; Michalsky, Elke; Preissner, Robert; Zeng, Yan; Gaedicke, Gerhard; Lode, Holger N

    2008-07-01

    Therapeutic vaccination against tumor antigens without induction of autoimmunity remains a major challenge in cancer immunotherapy. Here, we show for the first time effective therapeutic vaccination followed by suppression of established spontaneous neuroblastoma metastases using a tyrosine hydroxylase (TH) DNA minigene vaccine. We identified three novel mouse TH (mTH3) derived peptides with high predicted binding affinity to MHC class I antigen H2-K(k) according to the prediction program SYFPEITHI and computer modeling of epitopes into the MHC class I antigen binding groove. Subsequently, a DNA minigene vaccine was generated based on the expression vector pCMV-F3Ub encoding mutated ubiquitin (Gly(76) to Ala(76)) and mTH3. Prophylactic and therapeutic efficacies of this vaccine were established following oral delivery with attenuated Salmonella typhimurium SL7207. Only mice immunized with mTH3 were free of spontaneous liver metastases. This effect was clearly dependent on ubiquitin and high affinity of the mTH epitopes to MHC class I antigens. Specifically, we showed a crucial role for minigene expression as a stable ubiquitin-Ala(76) fusion peptide for vaccine efficacy. The immune response following the mTH3 DNA minigene vaccination was mediated by CD8(+) T cells as indicated by infiltration of primary tumors and TH-specific cytolytic activity in vitro. Importantly, no cell infiltration was detectable in TH-expressing adrenal medulla, indicating the absence of autoimmunity. In summary, we show effective therapeutic vaccination against neuroblastoma with a novel rationally designed TH minigene vaccine without induction of autoimmunity providing an important baseline for future clinical application of this strategy.

  4. Specifically Designed Constructed Wetlands: A Novel Treatment Approach for Scrubber Wastewater

    SciTech Connect

    John H. Rodgers Jr; James W. Castle; Chris Arrington: Derek Eggert; Meg Iannacone

    2005-09-01

    A pilot-scale wetland treatment system was specifically designed and constructed at Clemson University to evaluate removal of mercury, selenium, and other constituents from flue gas desulfurization (FGD) wastewater. Specific objectives of this research were: (1) to measure performance of a pilot-scale constructed wetland treatment system in terms of decreases in targeted constituents (Hg, Se and As) in the FGD wastewater from inflow to outflow; (2) to determine how the observed performance is achieved (both reactions and rates); and (3) to measure performance in terms of decreased bioavailability of these elements (i.e. toxicity of sediments in constructed wetlands and toxicity of outflow waters from the treatment system). Performance of the pilot-scale constructed wetland treatment systems was assessed using two criteria: anticipated NPDES permit levels and toxicity evaluations using two sentinel toxicity-testing organisms (Ceriodaphnia dubia and Pimephales promelas). These systems performed efficiently with varied inflow simulations of FGD wastewaters removing As, Hg, and Se concentrations below NPDES permit levels and reducing the toxicity of simulated FGD wastewater after treatment with the constructed wetland treatment systems. Sequential extraction procedures indicated that these elements (As, Hg, and Se) were bound to residual phases within sediments of these systems, which should limit their bioavailability to aquatic biota. Sediments collected from constructed wetland treatment systems were tested to observe toxicity to Hyalella azteca or Chironomus tetans. Complete survival (100%) was observed for H. azteca in all cells of the constructed wetland treatment system and C. tentans had an average of 91% survival over the three treatment cells containing sediments. Survival and growth of H. azteca and C. tentans did not differ significantly between sediments from the constructed wetland treatment system and controls. Since the sediments of the constructed

  5. Designing a C84 fullerene as a specific voltage-gated sodium channel blocker

    NASA Astrophysics Data System (ADS)

    Hilder, Tamsyn A.; Chung, Shin-Ho

    2013-07-01

    Fullerene derivatives demonstrate considerable potential for numerous biological applications, such as the effective inhibition of HIV protease. Recently, they were identified for their ability to indiscriminately block biological ion channels. A fullerene derivative which specifically blocks a particular ion channel could lead to a new set of drug leads for the treatment of various ion channel-related diseases. Here, we demonstrate their extraordinary potential by designing a fullerene which mimics some of the functions of μ-conotoxin, a peptide derived from cone snail venom which potently binds to the bacterial voltage-gated sodium channel (NavAb). We show, using molecular dynamics simulations, that the C84 fullerene with six lysine derivatives uniformly attached to its surface is selective to NavAb over a voltage-gated potassium channel (Kv1.3). The side chain of one of the lysine residues protrudes into the selectivity filter of the channel, while the methionine residues located just outside of the channel form hydrophobic contacts with the carbon atoms of the fullerene. The modified C84 fullerene strongly binds to the NavAb channel with an affinity of 46 nM but binds weakly to Kv1.3 with an affinity of 3 mM. This potent blocker of NavAb may serve as a structural template from which potent compounds can be designed for the targeting of mammalian Nav channels. There is a genuine need to target mammalian Nav channels as a form of treatment of various diseases which have been linked to their malfunction, such as epilepsy and chronic pain.

  6. 49 CFR 178.345 - General design and construction requirements applicable to Specification DOT 406 (§ 178.346), DOT...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... applicable to Specification DOT 406 (§ 178.346), DOT 407 (§ 178.347), and DOT 412 (§ 178.348) cargo tank....345 General design and construction requirements applicable to Specification DOT 406 (§ 178.346), DOT 407 (§ 178.347), and DOT 412 (§ 178.348) cargo tank motor vehicles....

  7. Computational Biology Tools for Identifying Specific Ligand Binding Residues for Novel Agrochemical and Drug Design.

    PubMed

    Neshich, Izabella Agostinho Pena; Nishimura, Leticia; de Moraes, Fabio Rogerio; Salim, Jose Augusto; Villalta-Romero, Fabian; Borro, Luiz; Yano, Inacio Henrique; Mazoni, Ivan; Tasic, Ljubica; Jardine, Jose Gilberto; Neshich, Goran

    2015-01-01

    The term "agrochemicals" is used in its generic form to represent a spectrum of pesticides, such as insecticides, fungicides or bactericides. They contain active components designed for optimized pest management and control, therefore allowing for economically sound and labor efficient agricultural production. A "drug" on the other side is a term that is used for compounds designed for controlling human diseases. Although drugs are subjected to much more severe testing and regulation procedures before reaching the market, they might contain exactly the same active ingredient as certain agrochemicals, what is the case described in present work, showing how a small chemical compound might be used to control pathogenicity of Gram negative bacteria Xylella fastidiosa which devastates citrus plantations, as well as for control of, for example, meningitis in humans. It is also clear that so far the production of new agrochemicals is not benefiting as much from the in silico new chemical compound identification/discovery as pharmaceutical production. Rational drug design crucially depends on detailed knowledge of structural information about the receptor (target protein) and the ligand (drug/agrochemical). The interaction between the two molecules is the subject of analysis that aims to understand relationship between structure and function, mainly deciphering some fundamental elements of the nanoenvironment where the interaction occurs. In this work we will emphasize the role of understanding nanoenvironmental factors that guide recognition and interaction of target protein and its function modifier, an agrochemical or a drug. The repertoire of nanoenvironment descriptors is used for two selected and specific cases we have approached in order to offer a technological solution for some very important problems that needs special attention in agriculture: elimination of pathogenicity of a bacterium which is attacking citrus plants and formulation of a new fungicide. Finally

  8. Computational Biology Tools for Identifying Specific Ligand Binding Residues for Novel Agrochemical and Drug Design.

    PubMed

    Neshich, Izabella Agostinho Pena; Nishimura, Leticia; de Moraes, Fabio Rogerio; Salim, Jose Augusto; Villalta-Romero, Fabian; Borro, Luiz; Yano, Inacio Henrique; Mazoni, Ivan; Tasic, Ljubica; Jardine, Jose Gilberto; Neshich, Goran

    2015-01-01

    The term "agrochemicals" is used in its generic form to represent a spectrum of pesticides, such as insecticides, fungicides or bactericides. They contain active components designed for optimized pest management and control, therefore allowing for economically sound and labor efficient agricultural production. A "drug" on the other side is a term that is used for compounds designed for controlling human diseases. Although drugs are subjected to much more severe testing and regulation procedures before reaching the market, they might contain exactly the same active ingredient as certain agrochemicals, what is the case described in present work, showing how a small chemical compound might be used to control pathogenicity of Gram negative bacteria Xylella fastidiosa which devastates citrus plantations, as well as for control of, for example, meningitis in humans. It is also clear that so far the production of new agrochemicals is not benefiting as much from the in silico new chemical compound identification/discovery as pharmaceutical production. Rational drug design crucially depends on detailed knowledge of structural information about the receptor (target protein) and the ligand (drug/agrochemical). The interaction between the two molecules is the subject of analysis that aims to understand relationship between structure and function, mainly deciphering some fundamental elements of the nanoenvironment where the interaction occurs. In this work we will emphasize the role of understanding nanoenvironmental factors that guide recognition and interaction of target protein and its function modifier, an agrochemical or a drug. The repertoire of nanoenvironment descriptors is used for two selected and specific cases we have approached in order to offer a technological solution for some very important problems that needs special attention in agriculture: elimination of pathogenicity of a bacterium which is attacking citrus plants and formulation of a new fungicide. Finally

  9. 77 FR 59626 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-28

    ... HIV services and medical treatment within 11 of the 12 Metropolitan Statistical Areas (MSAs) and... instruments are designed to collect information about: Specific program components; HIV testing integration... HEALTH SERVICES ADMINISTRATION Agency Information Collection Activities: Proposed Collection;...

  10. Site-specific integration and tailoring of cassette design for sustainable gene transfer.

    PubMed

    Lombardo, Angelo; Cesana, Daniela; Genovese, Pietro; Di Stefano, Bruno; Provasi, Elena; Colombo, Daniele F; Neri, Margherita; Magnani, Zulma; Cantore, Alessio; Lo Riso, Pietro; Damo, Martina; Pello, Oscar M; Holmes, Michael C; Gregory, Philip D; Gritti, Angela; Broccoli, Vania; Bonini, Chiara; Naldini, Luigi

    2011-08-21

    Integrative gene transfer methods are limited by variable transgene expression and by the consequences of random insertional mutagenesis that confound interpretation in gene-function studies and may cause adverse events in gene therapy. Site-specific integration may overcome these hurdles. Toward this goal, we studied the transcriptional and epigenetic impact of different transgene expression cassettes, targeted by engineered zinc-finger nucleases to the CCR5 and AAVS1 genomic loci of human cells. Analyses performed before and after integration defined features of the locus and cassette design that together allow robust transgene expression without detectable transcriptional perturbation of the targeted locus and its flanking genes in many cell types, including primary human lymphocytes. We thus provide a framework for sustainable gene transfer in AAVS1 that can be used for dependable genetic manipulation, neutral marking of the cell and improved safety of therapeutic applications, and demonstrate its feasibility by rapidly generating human lymphocytes and stem cells carrying targeted and benign transgene insertions.

  11. 34 CFR 403.100 - What are the requirements for designating a State corrections educational agency to administer...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF VOCATIONAL AND ADULT EDUCATION, DEPARTMENT OF EDUCATION STATE VOCATIONAL AND APPLIED TECHNOLOGY EDUCATION PROGRAM What... 34 Education 3 2011-07-01 2011-07-01 false What are the requirements for designating a...

  12. 77 FR 52744 - Food and Drug Administration/European Medicines Agency Orphan Product Designation and Grant Workshop

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-30

    ... Designation and Grant Workshop. This 1-day workshop is intended to provide valuable information about the FDA... check procedures will be performed. For parking and security information, please refer to http://www.fda.gov/AboutFDA/WorkingatFDA/BuildingsandFacilities/WhiteOakCampusInformation/ucm241740.htm ....

  13. Corporate Sector Practice Informs Online Workforce Training for Australian Government Agencies: Towards Effective Educational-Learning Systems Design

    ERIC Educational Resources Information Center

    McKay, Elspeth; Vilela, Cenie

    2011-01-01

    The purpose of this paper is to outline government online training practice. We searched individual research domains of the human-dimensions of Human Computer Interaction (HCI), information and communications technologies (ICT) and instructional design for evidence of either corporate sector or government training practices. We overlapped these…

  14. 34 CFR 403.100 - What are the requirements for designating a State corrections educational agency to administer...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF VOCATIONAL AND ADULT EDUCATION, DEPARTMENT OF EDUCATION STATE VOCATIONAL AND APPLIED TECHNOLOGY EDUCATION PROGRAM What... 34 Education 3 2010-07-01 2010-07-01 false What are the requirements for designating a...

  15. Synthesis and biochemical characterization of obligatory dimers of the sugar non-specific nuclease from Serratia marcescens using specifically designed bismaleimidoalkanes as SH-specific crosslinking reagents.

    PubMed

    Franke, I; Pingoud, A

    1999-01-01

    The genetically engineered S140C variant of the homodimeric nuclease from Serratia marcescens was crosslinked across the dimer interface at the Cys 140 residues using bifunctional SH-specific 1,1'-alkanediyl-bis-pyrrole-2,5-diones of different lengths. These bismaleimidoalkanes were synthesized by the condensation of n-alkyldiamines with maleic anhydride and subsequent cyclization with acetic anhydride and sodium acetate. Bismaleimidohexane (BMH) which gave the best crosslinking yield was used to produce in preparative amounts crosslinked Serratia nuclease. The crosslinked protein has the same secondary structure and exhibits the same guanidinium chloride unfolding behavior as the wild type enzyme or the non-covalently linked S 140C variant. In contrast, in thermal unfolding experiments the crosslinked dimer behaves differently from the wild type enzyme or the non-covalently linked S140C variant. CD-spectra recorded during temperature rise showed only minor changes of the secondary structure composition for the wild type enzyme and the non-covalently linked S140C variant, whereas in the case of the crosslinked S140C dimer a distinct increase of the CD effect was observed corresponding to an increase in alpha-helix. Our results demonstrate that bismaleimidoalkanes are very well suited to covalently link subunits of proteins, provided suitably located cysteine residues are present.

  16. Design of Protein Multi-specificity Using an Independent Sequence Search Reduces the Barrier to Low Energy Sequences.

    PubMed

    Sevy, Alexander M; Jacobs, Tim M; Crowe, James E; Meiler, Jens

    2015-07-01

    Computational protein design has found great success in engineering proteins for thermodynamic stability, binding specificity, or enzymatic activity in a 'single state' design (SSD) paradigm. Multi-specificity design (MSD), on the other hand, involves considering the stability of multiple protein states simultaneously. We have developed a novel MSD algorithm, which we refer to as REstrained CONvergence in multi-specificity design (RECON). The algorithm allows each state to adopt its own sequence throughout the design process rather than enforcing a single sequence on all states. Convergence to a single sequence is encouraged through an incrementally increasing convergence restraint for corresponding positions. Compared to MSD algorithms that enforce (constrain) an identical sequence on all states the energy landscape is simplified, which accelerates the search drastically. As a result, RECON can readily be used in simulations with a flexible protein backbone. We have benchmarked RECON on two design tasks. First, we designed antibodies derived from a common germline gene against their diverse targets to assess recovery of the germline, polyspecific sequence. Second, we design "promiscuous", polyspecific proteins against all binding partners and measure recovery of the native sequence. We show that RECON is able to efficiently recover native-like, biologically relevant sequences in this diverse set of protein complexes. PMID:26147100

  17. Design and control of energy efficient drying processes with specific reference to foods

    SciTech Connect

    Franzen, K.; Kim, M.; Liang, H.; Murakami, E.; Narsimhan, G.; Okos, M.; Singh, R.; Waananen, K.; Xiong, X.

    1988-07-15

    This report contains a detailed summary of all work performed to date. Task 10 involves a comprehensive review of drying theory. Proposed mass transfer mechanisms include liquid and vapor diffusion, capillary flow, surface diffusion, hydrodynamic flow, and evaporation/condensation processes. Pasta was chosen as a model system in this project since it is macroscopically homogenous and can be made under controlled conditions. Task 11 involves experimental drying studies. A high pressure drying apparatus is available for studies related to the revision of the fundamental drying model. The dryer will require two major modifications for the planned tests: installation of a pressure control valve and recirculation of exhaust gas. A tray dryer was used to measure the shrinkage coefficient of nonfat milk, and will be used for further tests on nonfat milk, as well as whey and tomato puree. A method of economic analysis regarding use of mechanical vapor recompression is presented. Task 12 involves food quality studies. A model of nonenzymatic browning (NEB) was developed based on NEB in skim milk samples containing 3.5--50% moisture, exposed to temperatures of 35--130{degrees}C. The browning rate was zero order after a lag period, and the temperature dependence fit an Arrhenius relation. The critical moisture occurs between 4% and 11% moisture. Task 13 addresses recommendations and strategies for dryer design and control. Moisture sensors were reviewed with specific reference to their on-line applicability. The IR sensor was found to be the most promising. Task 14 examined moisture mobility and interaction in foods. The BET adsorption method using nitrogen gas was applied to pasta, skim milk and egg albumin systems. The data obtained do not show good reproducibility, possibly due to an inadequate sample size. The possibility of using water vapor adsorption will be studied in future experiments. 210 refs., 30 figs., 22 tabs. (MHB)

  18. A Content Analysis of the Web Sites of the Fifty State Library Agencies of the United States of America.

    ERIC Educational Resources Information Center

    Berendsen, Nicole M.

    To determine what content and design features may be included in the new design of the State Library of Ohio's World Wide Web site, the researcher conducted a content analysis of the World Wide Web sites of the fifty state library agencies, using the COSLA (Chief Officers of State Library Agencies) index as the access point. Specifically, content…

  19. 24 CFR 3280.904 - Specific requirements for designing the transportation system.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... is usually of the socket type) shall be securely fastened to the drawbar in such a manner as to... loads as outlined in § 3280.904(b)(3) without exceeding maximum allowable stresses for design spring... design loads outlined in § 3280.904(b)(3) without exceeding maximum allowable stresses for design...

  20. Course Design and Delivery Specifications as a Tool for Ensuring Quality in an Online Training Program

    ERIC Educational Resources Information Center

    Docq, Françoise

    2015-01-01

    This case discusses the design, implementation, and regulation of a hybrid training program (60 credits over two years) organised by three business schools in Europe, and stretching over a five-year period. Following an incremental design process, the design team faced multiple challenges, from finding the added value of hybridization to choosing…