Science.gov

Sample records for agency designates specific

  1. 31 CFR 0.104 - Designated Agency Ethics Official and Alternate Designated Agency Ethics Official.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 31 Money and Finance: Treasury 1 2014-07-01 2014-07-01 false Designated Agency Ethics Official and Alternate Designated Agency Ethics Official. 0.104 Section 0.104 Money and Finance: Treasury Office of the... Responsibilities § 0.104 Designated Agency Ethics Official and Alternate Designated Agency Ethics Official....

  2. 31 CFR 0.104 - Designated Agency Ethics Official and Alternate Designated Agency Ethics Official.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 31 Money and Finance: Treasury 1 2012-07-01 2012-07-01 false Designated Agency Ethics Official and Alternate Designated Agency Ethics Official. 0.104 Section 0.104 Money and Finance: Treasury Office of the... Responsibilities § 0.104 Designated Agency Ethics Official and Alternate Designated Agency Ethics Official....

  3. 31 CFR 0.104 - Designated Agency Ethics Official and Alternate Designated Agency Ethics Official.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 31 Money and Finance: Treasury 1 2013-07-01 2013-07-01 false Designated Agency Ethics Official and Alternate Designated Agency Ethics Official. 0.104 Section 0.104 Money and Finance: Treasury Office of the... Responsibilities § 0.104 Designated Agency Ethics Official and Alternate Designated Agency Ethics Official....

  4. 31 CFR 0.104 - Designated Agency Ethics Official and Alternate Designated Agency Ethics Official.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Designated Agency Ethics Official and Alternate Designated Agency Ethics Official. 0.104 Section 0.104 Money and Finance: Treasury Office of the... Responsibilities § 0.104 Designated Agency Ethics Official and Alternate Designated Agency Ethics Official....

  5. 31 CFR 0.104 - Designated Agency Ethics Official and Alternate Designated Agency Ethics Official.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 31 Money and Finance: Treasury 1 2011-07-01 2011-07-01 false Designated Agency Ethics Official and Alternate Designated Agency Ethics Official. 0.104 Section 0.104 Money and Finance: Treasury Office of the... Responsibilities § 0.104 Designated Agency Ethics Official and Alternate Designated Agency Ethics Official....

  6. 29 CFR 2703.2 - Designated agency ethics official and alternate designated agency ethics official.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 9 2014-07-01 2014-07-01 false Designated agency ethics official and alternate designated agency ethics official. 2703.2 Section 2703.2 Labor Regulations Relating to Labor (Continued) FEDERAL... agency ethics official and alternate designated agency ethics official. The Chairman shall appoint...

  7. 29 CFR 2703.2 - Designated agency ethics official and alternate designated agency ethics official.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 9 2012-07-01 2012-07-01 false Designated agency ethics official and alternate designated agency ethics official. 2703.2 Section 2703.2 Labor Regulations Relating to Labor (Continued) FEDERAL... agency ethics official and alternate designated agency ethics official. The Chairman shall appoint...

  8. 29 CFR 2703.2 - Designated agency ethics official and alternate designated agency ethics official.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 9 2013-07-01 2013-07-01 false Designated agency ethics official and alternate designated agency ethics official. 2703.2 Section 2703.2 Labor Regulations Relating to Labor (Continued) FEDERAL... agency ethics official and alternate designated agency ethics official. The Chairman shall appoint...

  9. 29 CFR 2703.2 - Designated agency ethics official and alternate designated agency ethics official.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Designated agency ethics official and alternate designated agency ethics official. 2703.2 Section 2703.2 Labor Regulations Relating to Labor (Continued) FEDERAL... agency ethics official and alternate designated agency ethics official. The Chairman shall appoint...

  10. 29 CFR 2703.2 - Designated agency ethics official and alternate designated agency ethics official.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 9 2011-07-01 2011-07-01 false Designated agency ethics official and alternate designated agency ethics official. 2703.2 Section 2703.2 Labor Regulations Relating to Labor (Continued) FEDERAL... agency ethics official and alternate designated agency ethics official. The Chairman shall appoint...

  11. 29 CFR 1601.74 - Designated and notice agencies.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Commission has been designated as a FEP agency for all charges except charges alleging a violation of title... Relations Commission 3 3 The Austin (TX) Human Relations Commission has been designated as a FEP agency for... Personnel Board 4 4 The Colorado State Personnel Board has been designated as a FEP agency for only...

  12. 29 CFR 1601.74 - Designated and notice agencies.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Commission 3 3 The Arlington Human Rights Commission has been designated as a FEP agency for all charges... Commission has been designated as a FEP agency for all charges except charges alleging a violation of title... Board has been designated as a FEP agency for only those charges which relate to...

  13. 29 CFR 1601.74 - Designated and notice agencies.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Commission has been designated as a FEP agency for all charges except charges alleging a violation of title... Relations Commission 3 3 The Austin (TX) Human Relations Commission has been designated as a FEP agency for... Personnel Board 4 4 The Colorado State Personnel Board has been designated as a FEP agency for only...

  14. 43 CFR 46.220 - How to designate lead agencies.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 43 Public Lands: Interior 1 2012-10-01 2011-10-01 true How to designate lead agencies. 46.220... NATIONAL ENVIRONMENTAL POLICY ACT OF 1969 Initiating the NEPA Process § 46.220 How to designate lead agencies. (a) In most cases, the Responsible Official should designate one Federal agency as the lead...

  15. 43 CFR 46.220 - How to designate lead agencies.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 Public Lands: Interior 1 2011-10-01 2011-10-01 false How to designate lead agencies. 46.220... NATIONAL ENVIRONMENTAL POLICY ACT OF 1969 Initiating the NEPA Process § 46.220 How to designate lead agencies. (a) In most cases, the Responsible Official should designate one Federal agency as the lead...

  16. 34 CFR 303.142 - Designation of lead agency.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Designation of lead agency. 303.142 Section 303.142 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION....142 Designation of lead agency. Each application must include a designation of the lead agency in...

  17. 43 CFR 46.220 - How to designate lead agencies.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 43 Public Lands: Interior 1 2013-10-01 2013-10-01 false How to designate lead agencies. 46.220... NATIONAL ENVIRONMENTAL POLICY ACT OF 1969 Initiating the NEPA Process § 46.220 How to designate lead agencies. (a) In most cases, the Responsible Official should designate one Federal agency as the lead...

  18. 43 CFR 46.220 - How to designate lead agencies.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false How to designate lead agencies. 46.220... NATIONAL ENVIRONMENTAL POLICY ACT OF 1969 Initiating the NEPA Process § 46.220 How to designate lead agencies. (a) In most cases, the Responsible Official should designate one Federal agency as the lead...

  19. 43 CFR 46.220 - How to designate lead agencies.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 43 Public Lands: Interior 1 2014-10-01 2014-10-01 false How to designate lead agencies. 46.220... NATIONAL ENVIRONMENTAL POLICY ACT OF 1969 Initiating the NEPA Process § 46.220 How to designate lead agencies. (a) In most cases, the Responsible Official should designate one Federal agency as the lead...

  20. 34 CFR 303.142 - Designation of lead agency.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Designation of lead agency. 303.142 Section 303.142 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION....142 Designation of lead agency. Each application must include a designation of the lead agency in...

  1. 13 CFR 105.403 - Designated Agency Ethics Officials.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Designated Agency Ethics Officials... Agency Ethics Officials. The Designated Agency Ethics Official and Alternates administer the program for... advice and counsel regarding matters relating to the Ethics in Government Act of 1978 and...

  2. 13 CFR 105.403 - Designated Agency Ethics Officials.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Designated Agency Ethics Officials... Agency Ethics Officials. The Designated Agency Ethics Official and Alternates administer the program for... advice and counsel regarding matters relating to the Ethics in Government Act of 1978 and...

  3. 13 CFR 105.403 - Designated Agency Ethics Officials.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Designated Agency Ethics Officials... Agency Ethics Officials. The Designated Agency Ethics Official and Alternates administer the program for... advice and counsel regarding matters relating to the Ethics in Government Act of 1978 and...

  4. 13 CFR 105.403 - Designated Agency Ethics Officials.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Designated Agency Ethics Officials... Agency Ethics Officials. The Designated Agency Ethics Official and Alternates administer the program for... advice and counsel regarding matters relating to the Ethics in Government Act of 1978 and...

  5. 34 CFR 303.500 - Lead agency establishment or designation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Lead agency establishment or designation. 303.500... INFANTS AND TODDLERS WITH DISABILITIES State Administration General § 303.500 Lead agency establishment or designation. Each system must include a single line of responsibility in a lead agency that— (a)...

  6. 34 CFR 303.201 - Designation of lead agency.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true Designation of lead agency. 303.201 Section 303.201 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION... of lead agency. Each application must include the name of the State lead agency, as designated...

  7. 34 CFR 303.201 - Designation of lead agency.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false Designation of lead agency. 303.201 Section 303.201 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION... of lead agency. Each application must include the name of the State lead agency, as designated...

  8. 24 CFR 58.57 - Lead agency designation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Lead agency designation. 58.57 Section 58.57 Housing and Urban Development Office of the Secretary, Department of Housing and Urban....57 Lead agency designation. If there are several agencies ready to assume the lead role,...

  9. 24 CFR 58.57 - Lead agency designation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Lead agency designation. 58.57 Section 58.57 Housing and Urban Development Office of the Secretary, Department of Housing and Urban....57 Lead agency designation. If there are several agencies ready to assume the lead role,...

  10. 34 CFR 303.500 - Lead agency establishment or designation.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Lead agency establishment or designation. 303.500... INFANTS AND TODDLERS WITH DISABILITIES State Administration General § 303.500 Lead agency establishment or designation. Each system must include a single line of responsibility in a lead agency that— (a)...

  11. 34 CFR 303.201 - Designation of lead agency.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Designation of lead agency. 303.201 Section 303.201 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION... of lead agency. Each application must include the name of the State lead agency, as designated...

  12. 24 CFR 58.57 - Lead agency designation.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 1 2013-04-01 2013-04-01 false Lead agency designation. 58.57 Section 58.57 Housing and Urban Development Office of the Secretary, Department of Housing and Urban....57 Lead agency designation. If there are several agencies ready to assume the lead role,...

  13. 24 CFR 58.57 - Lead agency designation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 1 2012-04-01 2012-04-01 false Lead agency designation. 58.57 Section 58.57 Housing and Urban Development Office of the Secretary, Department of Housing and Urban....57 Lead agency designation. If there are several agencies ready to assume the lead role,...

  14. 24 CFR 58.57 - Lead agency designation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false Lead agency designation. 58.57 Section 58.57 Housing and Urban Development Office of the Secretary, Department of Housing and Urban....57 Lead agency designation. If there are several agencies ready to assume the lead role,...

  15. Reflight certification software design specifications

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The PDSS/IMC Software Design Specification for the Payload Development Support System (PDSS)/Image Motion Compensator (IMC) is contained. The PDSS/IMC is to be used for checkout and verification of the IMC flight hardware and software by NASA/MSFC.

  16. Organizing for Teacher Agency in Curricular Co-Design

    ERIC Educational Resources Information Center

    Severance, Samuel; Penuel, William R.; Sumner, Tamara; Leary, Heather

    2016-01-01

    Cultural-historical activity theory (CHAT) approaches to intervention aim for transformative agency, that is, collective actions that expand and bring about new possibilities for activity. In this article, we draw on CHAT as a resource for organizing design research that promotes teachers' agency in designing new science curriculum materials. We…

  17. 45 CFR 1321.33 - Designation of area agencies.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.33 Designation of... area agency on aging is designated, the State shall give right of first refusal to a unit of...

  18. 45 CFR 1321.33 - Designation of area agencies.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.33 Designation of... area agency on aging is designated, the State shall give right of first refusal to a unit of...

  19. 45 CFR 1321.33 - Designation of area agencies.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.33 Designation of... area agency on aging is designated, the State shall give right of first refusal to a unit of...

  20. 45 CFR 1321.33 - Designation of area agencies.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.33 Designation of... area agency on aging is designated, the State shall give right of first refusal to a unit of...

  1. 45 CFR 1321.33 - Designation of area agencies.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.33 Designation of... area agency on aging is designated, the State shall give right of first refusal to a unit of...

  2. 15 CFR 923.47 - Designated State agency.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... MANAGEMENT COASTAL ZONE MANAGEMENT PROGRAM REGULATIONS Authorities and Organization § 923.47 Designated State agency. (a) For program approval, the Governor of the state must designate a single state agency to receive and administer the grants for implementing the management program. (1) This entity must have...

  3. 15 CFR 923.47 - Designated State agency.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... MANAGEMENT COASTAL ZONE MANAGEMENT PROGRAM REGULATIONS Authorities and Organization § 923.47 Designated State agency. (a) For program approval, the Governor of the state must designate a single state agency to receive and administer the grants for implementing the management program. (1) This entity must have...

  4. 15 CFR 923.47 - Designated State agency.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... MANAGEMENT COASTAL ZONE MANAGEMENT PROGRAM REGULATIONS Authorities and Organization § 923.47 Designated State agency. (a) For program approval, the Governor of the state must designate a single state agency to receive and administer the grants for implementing the management program. (1) This entity must have...

  5. 15 CFR 923.47 - Designated State agency.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... MANAGEMENT COASTAL ZONE MANAGEMENT PROGRAM REGULATIONS Authorities and Organization § 923.47 Designated State agency. (a) For program approval, the Governor of the state must designate a single state agency to receive and administer the grants for implementing the management program. (1) This entity must have...

  6. 15 CFR 923.47 - Designated State agency.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... MANAGEMENT COASTAL ZONE MANAGEMENT PROGRAM REGULATIONS Authorities and Organization § 923.47 Designated State agency. (a) For program approval, the Governor of the state must designate a single state agency to receive and administer the grants for implementing the management program. (1) This entity must have...

  7. Federal Agencies and Design/Build Contracting.

    DTIC Science & Technology

    1991-07-01

    very large market for design/build contracting in the private 3 construction induitry. I I i £ I I I I i I I Arditi , David. Construction Productivity...continue its pursuit of design/build contracting and seek other alternative contracting methods to further increase its efficiency. I I BIBLIOGAAPHY Arditi

  8. 29 CFR 1601.74 - Designated and notice agencies.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... types of charges it shall be deemed a “Notice agency” pursuant to 29 CFR 1601.71(b). Austin (TX) Human Relations Commission 3 3 The Austin (TX) Human Relations Commission has been designated as a FEP agency...

  9. 50 CFR 402.07 - Designation of lead agency.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... ADMINISTRATION, DEPARTMENT OF COMMERCE); ENDANGERED SPECIES COMMITTEE REGULATIONS SUBCHAPTER A INTERAGENCY COOPERATION-ENDANGERED SPECIES ACT OF 1973, AS AMENDED General § 402.07 Designation of lead agency. When...

  10. 50 CFR 402.07 - Designation of lead agency.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... ADMINISTRATION, DEPARTMENT OF COMMERCE); ENDANGERED SPECIES COMMITTEE REGULATIONS SUBCHAPTER A INTERAGENCY COOPERATION-ENDANGERED SPECIES ACT OF 1973, AS AMENDED General § 402.07 Designation of lead agency. When...

  11. 50 CFR 402.07 - Designation of lead agency.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... ADMINISTRATION, DEPARTMENT OF COMMERCE); ENDANGERED SPECIES COMMITTEE REGULATIONS SUBCHAPTER A INTERAGENCY COOPERATION-ENDANGERED SPECIES ACT OF 1973, AS AMENDED General § 402.07 Designation of lead agency. When...

  12. 50 CFR 402.07 - Designation of lead agency.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... ADMINISTRATION, DEPARTMENT OF COMMERCE); ENDANGERED SPECIES COMMITTEE REGULATIONS SUBCHAPTER A INTERAGENCY COOPERATION-ENDANGERED SPECIES ACT OF 1973, AS AMENDED General § 402.07 Designation of lead agency. When...

  13. 50 CFR 402.07 - Designation of lead agency.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... ADMINISTRATION, DEPARTMENT OF COMMERCE); ENDANGERED SPECIES COMMITTEE REGULATIONS SUBCHAPTER A INTERAGENCY COOPERATION-ENDANGERED SPECIES ACT OF 1973, AS AMENDED General § 402.07 Designation of lead agency. When...

  14. 45 CFR 1386.34 - Designated State Agency.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Secretary for a review of the designation of the designated State agency if the Council's independence as an... State Developmental Disabilities Council, must give a 30 day written notice, by certified mail, to the... independence as an advocate is not assured because of the actions or inactions of the designated State...

  15. 45 CFR 1386.34 - Designated State Agency.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Secretary for a review of the designation of the designated State agency if the Council's independence as an... State Developmental Disabilities Council, must give a 30 day written notice, by certified mail, to the... independence as an advocate is not assured because of the actions or inactions of the designated State...

  16. 45 CFR 1386.34 - Designated State Agency.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Secretary for a review of the designation of the designated State agency if the Council's independence as an... State Developmental Disabilities Council, must give a 30 day written notice, by certified mail, to the... independence as an advocate is not assured because of the actions or inactions of the designated State...

  17. Evaluation of Regional Educational Agencies: General Design, Instruments, and Bibliography.

    ERIC Educational Resources Information Center

    Stepp, Ermel, Jr.

    One form of Regional Educational Agency (REA) is the Educational Cooperative, which was under field development for several years by the Appalachia Educational Laboratory, Inc. (AEL) and the United States Office of Education. The program included an evaluation effort, and the general design, instrumentation, and comprehensive bibliography for that…

  18. 21 CFR 3.4 - Designated agency component.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 1 2013-04-01 2013-04-01 false Designated agency component. 3.4 Section 3.4 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PRODUCT.... Where the primary mode of action is that of: (1) A drug (other than a biological product), the...

  19. 21 CFR 3.4 - Designated agency component.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 1 2012-04-01 2012-04-01 false Designated agency component. 3.4 Section 3.4 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PRODUCT.... Where the primary mode of action is that of: (1) A drug (other than a biological product), the...

  20. 41 CFR 101-5.104-5 - Designating agency representatives.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... each agency receiving a GSA notice regarding a scheduled feasibility study will be requested to designate one or more officials at the location where the study will be made who may consult with authorized... review of the feasibility of the proposed centralized service shall be made available to GSA through...

  1. 13 CFR 105.403 - Designated Agency Ethics Officials.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... advice and counsel regarding matters relating to the Ethics in Government Act of 1978 and its... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Designated Agency Ethics Officials. 105.403 Section 105.403 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION STANDARDS...

  2. 25 CFR 700.509 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Duties of the designated agency ethics official. 700.509... RELOCATION PROCEDURES Employee Responsibility and Conduct § 700.509 Duties of the designated agency ethics official. The Designated Agency Ethics Official shall coordinate and manage the agency's ethics...

  3. 25 CFR 700.509 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Duties of the designated agency ethics official. 700.509... RELOCATION PROCEDURES Employee Responsibility and Conduct § 700.509 Duties of the designated agency ethics official. The Designated Agency Ethics Official shall coordinate and manage the agency's ethics...

  4. 25 CFR 700.509 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Duties of the designated agency ethics official. 700.509... RELOCATION PROCEDURES Employee Responsibility and Conduct § 700.509 Duties of the designated agency ethics official. The Designated Agency Ethics Official shall coordinate and manage the agency's ethics...

  5. 25 CFR 700.509 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Duties of the designated agency ethics official. 700.509... RELOCATION PROCEDURES Employee Responsibility and Conduct § 700.509 Duties of the designated agency ethics official. The Designated Agency Ethics Official shall coordinate and manage the agency's ethics...

  6. Transitioning from conceptual design to construction performance specification

    NASA Astrophysics Data System (ADS)

    Jeffers, Paul; Warner, Mark; Craig, Simon; Hubbard, Robert; Marshall, Heather

    2012-09-01

    On successful completion of a conceptual design review by a funding agency or customer, there is a transition phase before construction contracts can be placed. The nature of this transition phase depends on the Project's approach to construction and the particular subsystem being considered. There are generically two approaches; project retention of design authority and issuance of build to print contracts, or issuance of subsystem performance specifications with controlled interfaces. This paper relates to the latter where a proof of concept (conceptual or reference design) is translated into performance based sub-system specifications for competitive tender. This translation is not a straightforward process and there are a number of different issues to consider in the process. This paper deals with primarily the Telescope mount and Enclosure subsystems. The main subjects considered in this paper are: • Typical status of design at Conceptual Design Review compared with the desired status of Specifications and Interface Control Documents at Request for Quotation. • Options for capture and tracking of system requirements flow down from science / operating requirements and sub-system requirements, and functional requirements derived from reference design. • Requirements that may come specifically from the contracting approach. • Methods for effective use of reference design work without compromising a performance based specification. • Management of project team's expectation relating to design. • Effects on cost estimates from reference design to actual. This paper is based on experience and lessons learned through this process on both the VISTA and the ATST projects.

  7. 28 CFR 81.4 - Referral of reports where the designated agency is not a law enforcement agency.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Referral of reports where the designated agency is not a law enforcement agency. 81.4 Section 81.4 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.4 Referral...

  8. 28 CFR 81.4 - Referral of reports where the designated agency is not a law enforcement agency.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Referral of reports where the designated agency is not a law enforcement agency. 81.4 Section 81.4 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.4 Referral...

  9. 28 CFR 81.4 - Referral of reports where the designated agency is not a law enforcement agency.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Referral of reports where the designated agency is not a law enforcement agency. 81.4 Section 81.4 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.4 Referral...

  10. 28 CFR 81.4 - Referral of reports where the designated agency is not a law enforcement agency.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Referral of reports where the designated agency is not a law enforcement agency. 81.4 Section 81.4 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.4 Referral...

  11. 28 CFR 81.4 - Referral of reports where the designated agency is not a law enforcement agency.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Referral of reports where the designated agency is not a law enforcement agency. 81.4 Section 81.4 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.4 Referral...

  12. Engineered waste-package-system design specification

    SciTech Connect

    Not Available

    1983-05-01

    This report documents the waste package performance requirements and geologic and waste form data bases used in developing the conceptual designs for waste packages for salt, tuff, and basalt geologies. The data base reflects the latest geotechnical information on the geologic media of interest. The parameters or characteristics specified primarily cover spent fuel, defense high-level waste, and commercial high-level waste forms. The specification documents the direction taken during the conceptual design activity. A separate design specification will be developed prior to the start of the preliminary design activity.

  13. 36 CFR 907.16 - Actions where lead Agency designation is necessary.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... proximity, the Corporation will seek designation as lead agency for those actions that directly relate to... qualifies as one for which the Corporation will seek designation as lead agency, the Corporation...

  14. 36 CFR 1010.15 - Actions where lead agency designation is necessary.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... functional interdependence or geographical proximity, the Trust will seek designation as lead agency for... which the Trust will seek designation as lead agency, the Trust will promptly consult with...

  15. Formalisms for user interface specification and design

    NASA Technical Reports Server (NTRS)

    Auernheimer, Brent J.

    1989-01-01

    The application of formal methods to the specification and design of human-computer interfaces is described. A broad outline of human-computer interface problems, a description of the field of cognitive engineering and two relevant research results, the appropriateness of formal specification techniques, and potential NASA application areas are described.

  16. Domain specific software design for decision aiding

    NASA Technical Reports Server (NTRS)

    Keller, Kirby; Stanley, Kevin

    1992-01-01

    McDonnell Aircraft Company (MCAIR) is involved in many large multi-discipline design and development efforts of tactical aircraft. These involve a number of design disciplines that must be coordinated to produce an integrated design and a successful product. Our interpretation of a domain specific software design (DSSD) is that of a representation or framework that is specialized to support a limited problem domain. A DSSD is an abstract software design that is shaped by the problem characteristics. This parallels the theme of object-oriented analysis and design of letting the problem model directly drive the design. The DSSD concept extends the notion of software reusability to include representations or frameworks. It supports the entire software life cycle and specifically leads to improved prototyping capability, supports system integration, and promotes reuse of software designs and supporting frameworks. The example presented in this paper is the task network architecture or design which was developed for the MCAIR Pilot's Associate program. The task network concept supported both module development and system integration within the domain of operator decision aiding. It is presented as an instance where a software design exhibited many of the attributes associated with DSSD concept.

  17. 40 CFR 55.15 - Specific designation of corresponding onshore areas.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 5 2011-07-01 2011-07-01 false Specific designation of corresponding onshore areas. 55.15 Section 55.15 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) OUTER CONTINENTAL SHELF AIR REGULATIONS § 55.15 Specific designation...

  18. 5 CFR 2638.203 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Duties of the designated agency ethics official. 2638.203 Section 2638.203 Administrative Personnel OFFICE OF GOVERNMENT ETHICS GOVERNMENT ETHICS OFFICE OF GOVERNMENT ETHICS AND EXECUTIVE AGENCY ETHICS PROGRAM RESPONSIBILITIES Designated Agency...

  19. 5 CFR 2638.203 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 3 2012-01-01 2012-01-01 false Duties of the designated agency ethics official. 2638.203 Section 2638.203 Administrative Personnel OFFICE OF GOVERNMENT ETHICS GOVERNMENT ETHICS OFFICE OF GOVERNMENT ETHICS AND EXECUTIVE AGENCY ETHICS PROGRAM RESPONSIBILITIES Designated Agency...

  20. 5 CFR 2638.203 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Duties of the designated agency ethics official. 2638.203 Section 2638.203 Administrative Personnel OFFICE OF GOVERNMENT ETHICS GOVERNMENT ETHICS OFFICE OF GOVERNMENT ETHICS AND EXECUTIVE AGENCY ETHICS PROGRAM RESPONSIBILITIES Designated Agency...

  1. 5 CFR 2638.203 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 3 2011-01-01 2011-01-01 false Duties of the designated agency ethics official. 2638.203 Section 2638.203 Administrative Personnel OFFICE OF GOVERNMENT ETHICS GOVERNMENT ETHICS OFFICE OF GOVERNMENT ETHICS AND EXECUTIVE AGENCY ETHICS PROGRAM RESPONSIBILITIES Designated Agency...

  2. 36 CFR 1010.15 - Actions where lead agency designation is necessary.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Actions where lead agency... ENVIRONMENTAL QUALITY § 1010.15 Actions where lead agency designation is necessary. (a) Consistent with 40 CFR... functional interdependence or geographical proximity, the Trust will seek designation as lead agency...

  3. 36 CFR 1010.15 - Actions where lead agency designation is necessary.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false Actions where lead agency... ENVIRONMENTAL QUALITY § 1010.15 Actions where lead agency designation is necessary. (a) Consistent with 40 CFR... functional interdependence or geographical proximity, the Trust will seek designation as lead agency...

  4. 36 CFR 907.16 - Actions where lead Agency designation is necessary.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false Actions where lead Agency... DEVELOPMENT CORPORATION ENVIRONMENTAL QUALITY § 907.16 Actions where lead Agency designation is necessary. (a... proximity, the Corporation will seek designation as lead agency for those actions that directly relate...

  5. 36 CFR 907.16 - Actions where lead Agency designation is necessary.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false Actions where lead Agency... DEVELOPMENT CORPORATION ENVIRONMENTAL QUALITY § 907.16 Actions where lead Agency designation is necessary. (a... proximity, the Corporation will seek designation as lead agency for those actions that directly relate...

  6. 36 CFR 1010.15 - Actions where lead agency designation is necessary.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 3 2013-07-01 2012-07-01 true Actions where lead agency... ENVIRONMENTAL QUALITY § 1010.15 Actions where lead agency designation is necessary. (a) Consistent with 40 CFR... functional interdependence or geographical proximity, the Trust will seek designation as lead agency...

  7. 36 CFR 907.16 - Actions where lead Agency designation is necessary.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Actions where lead Agency... DEVELOPMENT CORPORATION ENVIRONMENTAL QUALITY § 907.16 Actions where lead Agency designation is necessary. (a... proximity, the Corporation will seek designation as lead agency for those actions that directly relate...

  8. 36 CFR 1010.15 - Actions where lead agency designation is necessary.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false Actions where lead agency... ENVIRONMENTAL QUALITY § 1010.15 Actions where lead agency designation is necessary. (a) Consistent with 40 CFR... functional interdependence or geographical proximity, the Trust will seek designation as lead agency...

  9. 36 CFR 907.16 - Actions where lead Agency designation is necessary.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 3 2013-07-01 2012-07-01 true Actions where lead Agency... DEVELOPMENT CORPORATION ENVIRONMENTAL QUALITY § 907.16 Actions where lead Agency designation is necessary. (a... proximity, the Corporation will seek designation as lead agency for those actions that directly relate...

  10. 45 CFR 1321.35 - Withdrawal of area agency designation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... DEVELOPMENT SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.35 Withdrawal... established and published by the State agency on aging; or (4) Activities of the area agency are...

  11. 45 CFR 1321.35 - Withdrawal of area agency designation.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... DEVELOPMENT SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.35 Withdrawal... established and published by the State agency on aging; or (4) Activities of the area agency are...

  12. 45 CFR 1321.35 - Withdrawal of area agency designation.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... DEVELOPMENT SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.35 Withdrawal... established and published by the State agency on aging; or (4) Activities of the area agency are...

  13. 45 CFR 1321.35 - Withdrawal of area agency designation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... DEVELOPMENT SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.35 Withdrawal... established and published by the State agency on aging; or (4) Activities of the area agency are...

  14. 45 CFR 1321.35 - Withdrawal of area agency designation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... DEVELOPMENT SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION ON AGING, OLDER AMERICANS PROGRAMS GRANTS TO STATE AND COMMUNITY PROGRAMS ON AGING State Agency Responsibilities § 1321.35 Withdrawal... established and published by the State agency on aging; or (4) Activities of the area agency are...

  15. Space shuttle configuration accounting functional design specification

    NASA Technical Reports Server (NTRS)

    1974-01-01

    An analysis is presented of the requirements for an on-line automated system which must be capable of tracking the status of requirements and engineering changes and of providing accurate and timely records. The functional design specification provides the definition, description, and character length of the required data elements and the interrelationship of data elements to adequately track, display, and report the status of active configuration changes. As changes to the space shuttle program levels II and III configuration are proposed, evaluated, and dispositioned, it is the function of the configuration management office to maintain records regarding changes to the baseline and to track and report the status of those changes. The configuration accounting system will consist of a combination of computers, computer terminals, software, and procedures, all of which are designed to store, retrieve, display, and process information required to track proposed and proved engineering changes to maintain baseline documentation of the space shuttle program levels II and III.

  16. 5 CFR 6001.103 - Designation of separate agency components.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... teaching, speaking, or writing: (1) Federal Aviation Administration; (2) Federal Highway Administration; (3... identifying the DOT employee's agency under 5 CFR 2635.807 governing teaching, speaking, and writing....

  17. 5 CFR 3601.102 - Designation of separate agency components.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... outside sources and 5 CFR 2635.807 governing teaching, speaking and writing: (1) Armed Services Board of... identifying the DoD employee's agency under 5 CFR 2635.807 governing teaching, speaking and writing....

  18. 5 CFR 6001.103 - Designation of separate agency components.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... teaching, speaking, or writing: (1) Federal Aviation Administration; (2) Federal Highway Administration; (3... identifying the DOT employee's agency under 5 CFR 2635.807 governing teaching, speaking, and writing....

  19. 5 CFR 6001.103 - Designation of separate agency components.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... teaching, speaking, or writing: (1) Federal Aviation Administration; (2) Federal Highway Administration; (3... identifying the DOT employee's agency under 5 CFR 2635.807 governing teaching, speaking, and writing....

  20. 5 CFR 3601.102 - Designation of separate agency components.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... outside sources and 5 CFR 2635.807 governing teaching, speaking and writing: (1) Armed Services Board of... identifying the DoD employee's agency under 5 CFR 2635.807 governing teaching, speaking and writing....

  1. 5 CFR 3601.102 - Designation of separate agency components.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... outside sources and 5 CFR 2635.807 governing teaching, speaking and writing: (1) Armed Services Board of... identifying the DoD employee's agency under 5 CFR 2635.807 governing teaching, speaking and writing....

  2. 5 CFR 3601.102 - Designation of separate agency components.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... outside sources and 5 CFR 2635.807 governing teaching, speaking and writing: (1) Armed Services Board of... identifying the DoD employee's agency under 5 CFR 2635.807 governing teaching, speaking and writing....

  3. 5 CFR 6001.103 - Designation of separate agency components.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... teaching, speaking, or writing: (1) Federal Aviation Administration; (2) Federal Highway Administration; (3... identifying the DOT employee's agency under 5 CFR 2635.807 governing teaching, speaking, and writing....

  4. 5 CFR 6001.103 - Designation of separate agency components.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... teaching, speaking, or writing: (1) Federal Aviation Administration; (2) Federal Highway Administration; (3... identifying the DOT employee's agency under 5 CFR 2635.807 governing teaching, speaking, and writing....

  5. 5 CFR 3601.102 - Designation of separate agency components.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... outside sources and 5 CFR 2635.807 governing teaching, speaking and writing: (1) Armed Services Board of... identifying the DoD employee's agency under 5 CFR 2635.807 governing teaching, speaking and writing....

  6. 49 CFR 659.9 - Designation of oversight agency.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... agency representative of all states, to implement the requirements in this part; and (2) In the event... that the rail fixed guideway system is subject to a single program standard, adopted by all...

  7. 29 CFR 1601.80 - Certified designated FEP agencies.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... agencies receiving certification by the Commission are as follows: Alaska Commission for Human Rights Alexandria (VA) Human Rights Office Anchorage (AK) Equal Rights Commission Arizona Civil Rights Division Arlington County (VA) Human Rights Commission Austin Human Relations Commission Baltimore (MD)...

  8. 29 CFR 1601.80 - Certified designated FEP agencies.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... agencies receiving certification by the Commission are as follows: Alaska Commission for Human Rights Alexandria (VA) Human Rights Office Anchorage (AK) Equal Rights Commission Arizona Civil Rights Division Arlington County (VA) Human Rights Commission Austin Human Relations Commission Baltimore (MD)...

  9. 29 CFR 1601.80 - Certified designated FEP agencies.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... agencies receiving certification by the Commission are as follows: Alaska Commission for Human Rights Alexandria (VA) Human Rights Office Anchorage (AK) Equal Rights Commission Arizona Civil Rights Division Arlington County (VA) Human Rights Commission Austin Human Relations Commission Baltimore (MD)...

  10. 29 CFR 1601.80 - Certified designated FEP agencies.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... agencies receiving certification by the Commission are as follows: Alaska Commission for Human Rights Alexandria (VA) Human Rights Office Anchorage (AK) Equal Rights Commission Arizona Civil Rights Division Arlington County (VA) Human Rights Commission Austin Human Relations Commission Baltimore (MD)...

  11. Food Design Thinking: A Branch of Design Thinking Specific to Food Design

    ERIC Educational Resources Information Center

    Zampollo, Francesca; Peacock, Matthew

    2016-01-01

    Is there a need for a set of methods within Design Thinking tailored specifically for the Food Design process? Is there a need for a branch of Design Thinking dedicated to Food Design alone? Chefs are not generally trained in Design or Design Thinking, and we are only just beginning to understand how they ideate and what recourses are available to…

  12. Salt bridges: geometrically specific, designable interactions.

    PubMed

    Donald, Jason E; Kulp, Daniel W; DeGrado, William F

    2011-03-01

    Salt bridges occur frequently in proteins, providing conformational specificity and contributing to molecular recognition and catalysis. We present a comprehensive analysis of these interactions in protein structures by surveying a large database of protein structures. Salt bridges between Asp or Glu and His, Arg, or Lys display extremely well-defined geometric preferences. Several previously observed preferences are confirmed, and others that were previously unrecognized are discovered. Salt bridges are explored for their preferences for different separations in sequence and in space, geometric preferences within proteins and at protein-protein interfaces, co-operativity in networked salt bridges, inclusion within metal-binding sites, preference for acidic electrons, apparent conformational side chain entropy reduction on formation, and degree of burial. Salt bridges occur far more frequently between residues at close than distant sequence separations, but, at close distances, there remain strong preferences for salt bridges at specific separations. Specific types of complex salt bridges, involving three or more members, are also discovered. As we observe a strong relationship between the propensity to form a salt bridge and the placement of salt-bridging residues in protein sequences, we discuss the role that salt bridges might play in kinetically influencing protein folding and thermodynamically stabilizing the native conformation. We also develop a quantitative method to select appropriate crystal structure resolution and B-factor cutoffs. Detailed knowledge of these geometric and sequence dependences should aid de novo design and prediction algorithms.

  13. 29 CFR 1960.6 - Designation of agency safety and health officials.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 9 2011-07-01 2011-07-01 false Designation of agency safety and health officials. 1960.6 Section 1960.6 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH... AND HEALTH PROGRAMS AND RELATED MATTERS Administration § 1960.6 Designation of agency safety...

  14. Specifications and use of controlled low-strength material by state transportation agencies

    SciTech Connect

    Riggs, E.H.; Keck, R.H.

    1998-10-01

    Controlled low strength material, CLSM, was not used by state transportation agencies until recently, although its use in general construction dates back to the early 1970`s. The documentation of its present inclusion by many states is important in the progress of the technology of this relatively new material. States surveyed include Alabama, Florida, Georgia, North Carolina, South Carolina, and Virginia. The individual specifications are reviewed and compared. Testing methods are also discussed, along with representative data from laboratory and field test reports. Finally, a composite specification is suggested based on the consensus of the ones reviewed.

  15. Engineering stable cytoplasmic intrabodies with designed specificity.

    PubMed

    Donini, Marcello; Morea, Veronica; Desiderio, Angiola; Pashkoulov, Dimitre; Villani, Maria Elena; Tramontano, Anna; Benvenuto, Eugenio

    2003-07-04

    Many attempts have been made to develop antibody fragments that can be expressed in the cytoplasm ("intrabodies") in a stable and functional form. The recombinant antibody fragment scFv(F8) is characterised by peculiarly high in vitro stability and functional folding in both prokaryotic and eukaryotic cytoplasm. To dissect the relative contribution of different scFv(F8) regions to cytoplasmic stability and specificity we designed and constructed five chimeric molecules (scFv-P1 to P5) in which several groups of residues important for antigen binding in the poorly stable anti-hen egg lysozyme (HEL) scFv(D1.3) were progressively grafted onto the scFv(F8) scaffold. All five chimeric scFvs were expressed in a soluble form in the periplasm and cytoplasm of Escherichia coli. All the periplasmic oxidised forms and the scFv(P3) extracted from the cytoplasm in reducing conditions had HEL binding affinities essentially identical (K(d)=15nM) to that of the cognate scFv(D1.3) fragment (K(d)=16nM). The successful grafting of the antigen binding properties of D1.3 onto the scFv(F8) opens the road to the exploitation of this molecule as a scaffold for the reshaping of intrabodies with desired specificities to be targeted to the cytoplasm.

  16. Designing Micro- and Nanoswimmers for Specific Applications.

    PubMed

    Katuri, Jaideep; Ma, Xing; Stanton, Morgan M; Sánchez, Samuel

    2017-01-17

    Self-propelled colloids have emerged as a new class of active matter over the past decade. These are micrometer sized colloidal objects that transduce free energy from their surroundings and convert it to directed motion. The self-propelled colloids are in many ways, the synthetic analogues of biological self-propelled units such as algae or bacteria. Although they are propelled by very different mechanisms, biological swimmers are typically powered by flagellar motion and synthetic swimmers are driven by local chemical reactions, they share a number of common features with respect to swimming behavior. They exhibit run-and-tumble like behavior, are responsive to environmental stimuli, and can even chemically interact with nearby swimmers. An understanding of self-propelled colloids could help us in understanding the complex behaviors that emerge in populations of natural microswimmers. Self-propelled colloids also offer some advantages over natural microswimmers, since the surface properties, propulsion mechanisms, and particle geometry can all be easily modified to meet specific needs. From a more practical perspective, a number of applications, ranging from environmental remediation to targeted drug delivery, have been envisioned for these systems. These applications rely on the basic functionalities of self-propelled colloids: directional motion, sensing of the local environment, and the ability to respond to external signals. Owing to the vastly different nature of each of these applications, it becomes necessary to optimize the design choices in these colloids. There has been a significant effort to develop a range of synthetic self-propelled colloids to meet the specific conditions required for different processes. Tubular self-propelled colloids, for example, are ideal for decontamination processes, owing to their bubble propulsion mechanism, which enhances mixing in systems, but are incompatible with biological systems due to the toxic propulsion fuel and

  17. Designing Micro- and Nanoswimmers for Specific Applications

    PubMed Central

    2016-01-01

    Conspectus Self-propelled colloids have emerged as a new class of active matter over the past decade. These are micrometer sized colloidal objects that transduce free energy from their surroundings and convert it to directed motion. The self-propelled colloids are in many ways, the synthetic analogues of biological self-propelled units such as algae or bacteria. Although they are propelled by very different mechanisms, biological swimmers are typically powered by flagellar motion and synthetic swimmers are driven by local chemical reactions, they share a number of common features with respect to swimming behavior. They exhibit run-and-tumble like behavior, are responsive to environmental stimuli, and can even chemically interact with nearby swimmers. An understanding of self-propelled colloids could help us in understanding the complex behaviors that emerge in populations of natural microswimmers. Self-propelled colloids also offer some advantages over natural microswimmers, since the surface properties, propulsion mechanisms, and particle geometry can all be easily modified to meet specific needs. From a more practical perspective, a number of applications, ranging from environmental remediation to targeted drug delivery, have been envisioned for these systems. These applications rely on the basic functionalities of self-propelled colloids: directional motion, sensing of the local environment, and the ability to respond to external signals. Owing to the vastly different nature of each of these applications, it becomes necessary to optimize the design choices in these colloids. There has been a significant effort to develop a range of synthetic self-propelled colloids to meet the specific conditions required for different processes. Tubular self-propelled colloids, for example, are ideal for decontamination processes, owing to their bubble propulsion mechanism, which enhances mixing in systems, but are incompatible with biological systems due to the toxic

  18. Compiler writing system detail design specification. Volume 1: Language specification

    NASA Technical Reports Server (NTRS)

    Arthur, W. J.

    1974-01-01

    Construction within the Meta language for both language and target machine specification is reported. The elements of the function language as a meaning and syntax are presented, and the structure of the target language is described which represents the target dependent object text representation of applications programs.

  19. Southern routes for high-level radioactive waste: Agencies, contacts, and designations

    SciTech Connect

    Not Available

    1991-05-01

    The Southern Routes for High-Level Radioactive Waste: Agencies, Contacts and Designations is a compendium of sixteen southern states' routing programs for the transportation of high-level radioactive materials. The report identifies the state-designated routing agencies as defined under 49 Code of Federal Regulations (CFR) Part 171 and provides a reference to the source and scope of the agencies' rulemaking authority. Additionally, the state agency and contact designated by the state's governor to receive advance notification and shipment routing information under 10 CFR Parts 71 and 73 are also listed. This report also examines alternative route designations made by southern states and the lessons that were learned from the designation process.

  20. Southern routes for high-level radioactive waste: Agencies, contacts, and designations

    SciTech Connect

    Not Available

    1991-05-01

    The Southern Routes for High-Level Radioactive Waste: Agencies, Contacts and Designations is a compendium of sixteen southern states` routing programs for the transportation of high-level radioactive materials. The report identifies the state-designated routing agencies as defined under 49 Code of Federal Regulations (CFR) Part 171 and provides a reference to the source and scope of the agencies` rulemaking authority. Additionally, the state agency and contact designated by the state`s governor to receive advance notification and shipment routing information under 10 CFR Parts 71 and 73 are also listed. This report also examines alternative route designations made by southern states and the lessons that were learned from the designation process.

  1. 44 CFR 60.25 - Designation, duties, and responsibilities of State Coordinating Agencies.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 44 Emergency Management and Assistance 1 2011-10-01 2011-10-01 false Designation, duties, and responsibilities of State Coordinating Agencies. 60.25 Section 60.25 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY INSURANCE AND HAZARD MITIGATION National...

  2. 44 CFR 60.25 - Designation, duties, and responsibilities of State Coordinating Agencies.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Designation, duties, and responsibilities of State Coordinating Agencies. 60.25 Section 60.25 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY INSURANCE AND HAZARD MITIGATION National...

  3. Simplified spacecraft vulnerability assessments at component level in early design phase at the European Space Agency's Concurrent Design Facility

    NASA Astrophysics Data System (ADS)

    Kempf, Scott; Schäfer, Frank K.; Cardone, Tiziana; Ferreira, Ivo; Gerené, Sam; Destefanis, Roberto; Grassi, Lilith

    2016-12-01

    During recent years, the state-of-the-art risk assessment of the threat posed to spacecraft by micrometeoroids and space debris has been expanded to the analysis of failure modes of internal spacecraft components. This method can now be used to perform risk analyses for satellites to assess various failure levels - from failure of specific sub-systems to catastrophic break-up. This new assessment methodology is based on triple-wall ballistic limit equations (BLEs), specifically the Schäfer-Ryan-Lambert (SRL) BLE, which is applicable for describing failure threshold levels for satellite components following a hypervelocity impact. The methodology is implemented in the form of the software tool Particle Impact Risk and vulnerability Analysis Tool (PIRAT). During a recent European Space Agency (ESA) funded study, the PIRAT functionality was expanded in order to provide an interface to ESA's Concurrent Design Facility (CDF). The additions include a geometry importer and an OCDT (Open Concurrent Design Tool) interface. The new interface provides both the expanded geometrical flexibility, which is provided by external computer aided design (CAD) modelling, and an ease of import of existing data without the need for extensive preparation of the model. The reduced effort required to perform vulnerability analyses makes it feasible for application during early design phase, at which point modifications to satellite design can be undertaken with relatively little extra effort. The integration of PIRAT in the CDF represents the first time that vulnerability analyses can be performed in-session in ESA's CDF and the first time that comprehensive vulnerability studies can be applied cost-effectively in early design phase in general.

  4. 25 CFR 700.509 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... provide the OGE with records, reports and any other information which may be required under the Ethics in... Administration. If the Designated Agency Ethics Official receives a request which he or she believes should...

  5. 45 CFR 205.58 - Income and eligibility information; specific agreements required between the State agency and the...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Income and eligibility information; specific agreements required between the State agency and the agency supplying the information. 205.58 Section 205.58 Public Welfare Regulations Relating to Public Welfare OFFICE OF FAMILY ASSISTANCE (ASSISTANCE PROGRAMS), ADMINISTRATION FOR CHILDREN AND...

  6. 76 FR 81955 - Assessment Questionnaire-IP Sector Specific Agency Risk Self Assessment Tool (IP-SSARSAT)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-29

    ... SECURITY Assessment Questionnaire--IP Sector Specific Agency Risk Self Assessment Tool (IP-SSARSAT) AGENCY... Information Collection Request: 1670-NEW. SUMMARY: The Department of Homeland Security (DHS), National... received must include the words ``Department of Homeland Security'' and the docket ] number for this...

  7. 77 FR 33227 - Assessment Questionnaire-IP Sector Specific Agency Risk Self Assessment Tool (IP-SSARSAT)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-05

    ... SECURITY Assessment Questionnaire--IP Sector Specific Agency Risk Self Assessment Tool (IP-SSARSAT) AGENCY... Information Collection Request, 1670-NEW. SUMMARY: The Department of Homeland Security (DHS), National... words ``Department of Homeland Security'' and the docket number for this action. Comments received...

  8. 46 CFR 162.050-21 - Separator: Design specification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Separator: Design specification. 162.050-21 Section 162... Separator: Design specification. (a) A separator must be designed to operate in each plane that forms an.... (c) Each separator component that is a moving part must be designed so that its movement...

  9. 46 CFR 162.050-21 - Separator: Design specification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 6 2011-10-01 2011-10-01 false Separator: Design specification. 162.050-21 Section 162... Separator: Design specification. (a) A separator must be designed to operate in each plane that forms an.... (c) Each separator component that is a moving part must be designed so that its movement...

  10. 46 CFR 162.050-21 - Separator: Design specification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 6 2014-10-01 2014-10-01 false Separator: Design specification. 162.050-21 Section 162... Separator: Design specification. (a) A separator must be designed to operate in each plane that forms an.... (c) Each separator component that is a moving part must be designed so that its movement...

  11. Radiological containment selection, design, and specification guide

    SciTech Connect

    Brown, R.L.

    1994-11-01

    This document provides guidance to Tank Waste Remediation Systems personnel in determining what containment is appropriate for work activities, what containments are available, general applications of each, design criteria, and other information needed to make informed decisions concerning containment application.

  12. As-built design specification for MISMAP

    NASA Technical Reports Server (NTRS)

    Brown, P. M.; Cheng, D. E.; Tompkins, M. A. (Principal Investigator)

    1981-01-01

    The MISMAP program, which is part of the CLASFYT package, is described. The program is designed to compare classification values with ground truth values for a segment and produce a comparison map and summary table.

  13. IMCS reflight certification requirements and design specifications

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The requirements for reflight certification are established. Software requirements encompass the software programs that are resident in the PCC, DEP, PDSS, EC, or any related GSE. A design approach for the reflight software packages is recommended. These designs will be of sufficient detail to permit the implementation of reflight software. The PDSS/IMC Reflight Certification system provides the tools and mechanisms for the user to perform the reflight certification test procedures, test data capture, test data display, and test data analysis. The system as defined will be structured to permit maximum automation of reflight certification procedures and test data analysis.

  14. 46 CFR 162.050-33 - Bilge alarm: Design specification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Bilge alarm: Design specification. 162.050-33 Section....050-33 Bilge alarm: Design specification. (a) This section contains requirements that apply to bilge alarms. (b) Each bilge alarm must be designed to meet the requirements for an oil content meter in §...

  15. 46 CFR 162.050-33 - Bilge alarm: Design specification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 6 2011-10-01 2011-10-01 false Bilge alarm: Design specification. 162.050-33 Section....050-33 Bilge alarm: Design specification. (a) This section contains requirements that apply to bilge alarms. (b) Each bilge alarm must be designed to meet the requirements for an oil content meter in §...

  16. 46 CFR 162.050-25 - Cargo monitor: Design specification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 6 2011-10-01 2011-10-01 false Cargo monitor: Design specification. 162.050-25 Section....050-25 Cargo monitor: Design specification. (a) This section contains requirements that apply to cargo monitors. (b) Each monitor must be designed so that it is calibrated by a means that does not...

  17. 49 CFR 107.402 - Application for designation as a certification agency.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... authority to U.S. Citizens or organizations having designations under this subpart from PHMSA. (d) Fireworks... Agency applicant must— (i) Be a U.S. citizen, or for non-U.S. citizens, have a designated U.S. agent... fireworks or explosives and a degree in the physical sciences or engineering from an accredited...

  18. 49 CFR 107.402 - Application for designation as a certification agency.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... authority to U.S. Citizens or organizations having designations under this subpart from PHMSA. (d) Fireworks... Agency applicant must— (i) Be a U.S. citizen, or for non-U.S. citizens, have a designated U.S. agent... fireworks or explosives and a degree in the physical sciences or engineering from an accredited...

  19. 75 FR 42094 - Agency Information Collection Activities; Proposed Collection; Comment Request; Designated New...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-20

    ... Collection; Comment Request; Designated New Animal Drugs for Minor Use and Minor Species AGENCY: Food and..., ] when appropriate, and other forms of information technology. Designated New Animal Drugs for Minor Use... authorize FDA to establish new regulatory procedures intended to make more medications legally available...

  20. AFEII Analog Front End Board Design Specifications

    SciTech Connect

    Rubinov, Paul; /Fermilab

    2005-04-01

    This document describes the design of the 2nd iteration of the Analog Front End Board (AFEII), which has the function of receiving charge signals from the Central Fiber Tracker (CFT) and providing digital hit pattern and charge amplitude information from those charge signals. This second iteration is intended to address limitations of the current AFE (referred to as AFEI in this document). These limitations become increasingly deleterious to the performance of the Central Fiber Tracker as instantaneous luminosity increases. The limitations are inherent in the design of the key front end chips on the AFEI board (the SVXIIe and the SIFT) and the architecture of the board itself. The key limitations of the AFEI are: (1) SVX saturation; (2) Discriminator to analog readout cross talk; (3) Tick to tick pedestal variation; and (4) Channel to channel pedestal variation. The new version of the AFE board, AFEII, addresses these limitations by use of a new chip, the TriP-t and by architectural changes, while retaining the well understood and desirable features of the AFEI board.

  1. A compressor designed for the energy research and development agency automotive gas turbine program

    NASA Technical Reports Server (NTRS)

    Galvas, M. R.

    1975-01-01

    A centrifugal compressor was designed for a gas turbine powered automobile as part of the Energy Research and Development Agency program to demonstrate emissions characteristics that meet 1978 standards with fuel economy and acceleration which are competitive with conventionally powered vehicles. A backswept impeller was designed for the compressor in order to attain the efficiency goal range required for the objectives of this program. Details of the design and method of flow analysis of the compressor are presented.

  2. Gateway design specification for fiber optic local area networks

    NASA Technical Reports Server (NTRS)

    1985-01-01

    This is a Design Specification for a gateway to interconnect fiber optic local area networks (LAN's). The internetworking protocols for a gateway device that will interconnect multiple local area networks are defined. This specification serves as input for preparation of detailed design specifications for the hardware and software of a gateway device. General characteristics to be incorporated in the gateway such as node address mapping, packet fragmentation, and gateway routing features are described.

  3. 45 CFR 1386.20 - Designated State Protection and Advocacy agency.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... proposed redesignation using the State register, State-wide newspapers, public service announcements on... 45 Public Welfare 4 2011-10-01 2011-10-01 false Designated State Protection and Advocacy agency. 1386.20 Section 1386.20 Public Welfare Regulations Relating to Public Welfare (Continued) OFFICE...

  4. 77 FR 55488 - Agency Information Collection Activities: Designation of Attorney in Fact

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-10

    ... SECURITY United States Immigration and Customs Enforcement Agency Information Collection Activities: Designation of Attorney in Fact The Department of Homeland Security, U.S. Immigration and Customs Enforcement... Budget. Comments should be addressed to OMB Desk Officer, for United States Immigration and...

  5. Analytics for Knowledge Creation: Towards Epistemic Agency and Design-Mode Thinking

    ERIC Educational Resources Information Center

    Chen, Bodong; Zhang, Jianwei

    2016-01-01

    Innovation and knowledge creation call for high-level epistemic agency and design-mode thinking, two competencies beyond the traditional scopes of schooling. In this paper, we discuss the need for learning analytics to support these two competencies, and more broadly, the demand for education for innovation. We ground these arguments on a…

  6. 34 CFR 370.7 - What shall the designated agency do to make its services accessible?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false What shall the designated agency do to make its services accessible? 370.7 Section 370.7 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF...

  7. 34 CFR 370.7 - What shall the designated agency do to make its services accessible?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false What shall the designated agency do to make its services accessible? 370.7 Section 370.7 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF...

  8. 34 CFR 370.7 - What shall the designated agency do to make its services accessible?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false What shall the designated agency do to make its services accessible? 370.7 Section 370.7 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF...

  9. 34 CFR 370.7 - What shall the designated agency do to make its services accessible?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true What shall the designated agency do to make its services accessible? 370.7 Section 370.7 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION...

  10. 34 CFR 370.7 - What shall the designated agency do to make its services accessible?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true What shall the designated agency do to make its services accessible? 370.7 Section 370.7 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION...

  11. 76 FR 31683 - Agency Information Collection (Designation of Certifying Official(s)) Activity Under OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-01

    ...: Extension of a currently approved collection. Abstracts: a. Educational institutions and job training... designated certifying official. b. Educational institution complete VA Form 22-8794a when there is a change to their financial institution. An agency may not conduct or sponsor, and a person is not required...

  12. 41 CFR 102-83.120 - What happens if an agency has a need to be in a specific urban area that is not a central city in...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... agency has a need to be in a specific urban area that is not a central city in a metropolitan area? 102... is not a central city in a metropolitan area? If an agency has a need to be in a specific urban area that is not a central city in a metropolitan area, then the agency must give first consideration...

  13. UXO Engineering Design. Technical Specification and ConceptualDesign

    SciTech Connect

    Beche, J-F.; Doolittle, L.; Greer, J.; Lafever, R.; Radding, Z.; Ratti, A.; Yaver, H.; Zimmermann, S.

    2005-04-23

    The design and fabrication of the UXO detector has numerous challenges and is an important component to the success of this study. This section describes the overall engineering approach, as well as some of the technical details that brought us to the present design. In general, an array of sensor coils is measuring the signal generated by the UXO object in response to a stimulation provided by the driver coil. The information related to the location, shape and properties of the object is derived from the analysis of the measured data. Each sensor coil is instrumented with a waveform digitizer operating at a nominal digitization rate of 100 kSamples per second. The sensor coils record both the large transient pulse of the driver coil and the UXO object response pulse. The latter is smaller in amplitude and must be extracted from the large transient signal. The resolution required is 16 bits over a dynamic range of at least 140 dB. The useful signal bandwidth of the application extends from DC to 40 kHz. The low distortion of each component is crucial in order to maintain an excellent linearity over the full dynamic range and to minimize the calibration procedure. The electronics must be made as compact as possible so that the response of its metallic parts has a minimum signature response. Also because of a field system portability requirement, the power consumption of the instrument must be kept as low as possible. The theory and results of numerical and experimental studies that led to the proof-of-principle multitransmitter-multireceiver Active ElectroMagnetic (AEM) system, that can not only accurately detect but also characterize and discriminate UXO targets, are summarized in LBNL report-53962: ''Detection and Classification of Buried Metallic Objects, UX-1225''.

  14. Working Notes from the 1992 AAAI Workshop on Automating Software Design. Theme: Domain Specific Software Design

    NASA Technical Reports Server (NTRS)

    Keller, Richard M. (Editor); Barstow, David; Lowry, Michael R.; Tong, Christopher H.

    1992-01-01

    The goal of this workshop is to identify different architectural approaches to building domain-specific software design systems and to explore issues unique to domain-specific (vs. general-purpose) software design. Some general issues that cut across the particular software design domain include: (1) knowledge representation, acquisition, and maintenance; (2) specialized software design techniques; and (3) user interaction and user interface.

  15. SEPAC flight software detailed design specifications, volume 1

    NASA Technical Reports Server (NTRS)

    1982-01-01

    The detailed design specifications (as built) for the SEPAC Flight Software are defined. The design includes a description of the total software system and of each individual module within the system. The design specifications describe the decomposition of the software system into its major components. The system structure is expressed in the following forms: the control-flow hierarchy of the system, the data-flow structure of the system, the task hierarchy, the memory structure, and the software to hardware configuration mapping. The component design description includes details on the following elements: register conventions, module (subroutines) invocaton, module functions, interrupt servicing, data definitions, and database structure.

  16. 45 CFR 90.43 - What specific responsibilities do agencies and recipients have to ensure compliance with the Act?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... recipients have to ensure compliance with the Act? 90.43 Section 90.43 Public Welfare DEPARTMENT OF HEALTH....43 What specific responsibilities do agencies and recipients have to ensure compliance with the Act... written notice to each recipient of its obligations under the Act. The notice shall include a...

  17. 41 CFR 102-74.360 - What are the specific accident and fire prevention responsibilities of occupant agencies?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... accident and fire prevention responsibilities of occupant agencies? 102-74.360 Section 102-74.360 Public... MANAGEMENT REGULATION REAL PROPERTY 74-FACILITY MANAGEMENT Facility Management Accident and Fire Prevention § 102-74.360 What are the specific accident and fire prevention responsibilities of occupant...

  18. 41 CFR 102-74.360 - What are the specific accident and fire prevention responsibilities of occupant agencies?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... accident and fire prevention responsibilities of occupant agencies? 102-74.360 Section 102-74.360 Public... MANAGEMENT REGULATION REAL PROPERTY 74-FACILITY MANAGEMENT Facility Management Accident and Fire Prevention § 102-74.360 What are the specific accident and fire prevention responsibilities of occupant...

  19. ERSYS-SPP access method subsystem design specification

    NASA Technical Reports Server (NTRS)

    Weise, R. C. (Principal Investigator)

    1980-01-01

    The STARAN special purpose processor (SPP) is a machine allowing the same operation to be performed on up to 512 different data elements simultaneously. In the ERSYS system, it is to be attached to a 4341 plug compatible machine (PCM) to do certain existing algorithms and, at a later date, to perform other to be specified algorithms. That part of the interface between the 4341 PCM and the SPP located in the 4341 PCM is known as the SPP access method (SPPAM). Access to the SPPAM will be obtained by use of the NQUEUE and DQUEUE commands. The subsystem design specification is to incorporate all applicable design considerations from the ERSYS system design specification and the Level B requirements documents relating to the SPPAM. It is intended as a basis for the preliminary design review and will expand into the subsystem detailed design specification.

  20. A Learning Design Ontology Based on the IMS Specification

    ERIC Educational Resources Information Center

    Amorim, Ricardo R.; Lama, Manuel; Sanchez, Eduardo; Riera, Adolfo; Vila, Xose A.

    2006-01-01

    In this paper, we present an ontology to represent the semantics of the IMS Learning Design (IMS LD) specification, a meta-language used to describe the main elements of the learning design process. The motivation of this work relies on the expressiveness limitations found on the current XML-Schema implementation of the IMS LD conceptual model. To…

  1. 40 CFR 33.406 - May a recipient designate a lead agency for fair share objective negotiation purposes?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false May a recipient designate a lead agency for fair share objective negotiation purposes? 33.406 Section 33.406 Protection of Environment... recipient designate a lead agency for fair share objective negotiation purposes? If an Indian Tribal,...

  2. 75 FR 36067 - Agency Information Collection Activities; Proposed Collection; Comment Request; Chemical-Specific...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-24

    ... Rules, TSCA Section 8(a); EPA ICR No. 1198.09, OMB Control No. 2070-0067 AGENCY: Environmental....S.C. 3501 et seq.), this document announces that EPA is planning to submit a request to renew an... and OMB Control No. 2070-0067, is scheduled to expire on January 31, 2011. Before submitting the...

  3. Designing Games, Designing Roles: A Study of Youth Agency in an Urban Informal Education Program

    ERIC Educational Resources Information Center

    Sheridan, Kimberly M.; Clark, Kevin; Williams, Asia

    2013-01-01

    Collaboration (GDMC), an informal education program in 3D computer modeling and 2D interactive game design serving primarily African American youth aged 7 to 19 years in the Washington, D.C. metro area, transformed from a program designed and taught by adults to one designed and taught by youth. In Year 1, 8% of youth participants held a…

  4. Specific PCR product primer design using memetic algorithm.

    PubMed

    Yang, Cheng-Hong; Cheng, Yu-Huei; Chuang, Li-Yeh; Chang, Hsueh-Wei

    2009-01-01

    To provide feasible primer sets for performing a polymerase chain reaction (PCR) experiment, many primer design methods have been proposed. However, the majority of these methods require a relatively long time to obtain an optimal solution since large quantities of template DNA need to be analyzed. Furthermore, the designed primer sets usually do not provide a specific PCR product size. In recent years, evolutionary computation has been applied to PCR primer design and yielded promising results. In this article, a memetic algorithm (MA) is proposed to solve primer design problems associated with providing a specific product size for PCR experiments. The MA is compared with a genetic algorithm (GA) using an accuracy formula to estimate the quality of the primer design and test the running time. Overall, 50 accession nucleotide sequences were sampled for the comparison of the accuracy of the GA and MA for primer design. Five hundred runs of the GA and MA primer design were performed with PCR product lengths of 150-300 bps and 500-800 bps, and two different methods of calculating T(m) for each accession nucleotide sequence were tested. A comparison of the accuracy results for the GA and MA primer design showed that the MA primer design yielded better results than the GA primer design. The results further indicate that the proposed method finds optimal or near-optimal primer sets and effective PCR products in a dry dock experiment. Related materials are available online at http://bio.kuas.edu.tw/ma-pd/.

  5. Introduction to Psychology and Leadership. Design Specifications Document Including Specifications for Product and Course Design System Management and Evaluation Procedures.

    ERIC Educational Resources Information Center

    Westinghouse Learning Corp., Annapolis, MD.

    The design specifications for the United States Naval Academy leadership course developed by Westinghouse Learning Corporation are presented in this report, covering course system design, management, and evaluation. EM 010 418 through EM 010 447 and EM 010 451 through EM 010 512 are related documents, with the final report appearing under EM 010…

  6. Designing Domain-Specific HUMS Architectures: An Automated Approach

    NASA Technical Reports Server (NTRS)

    Mukkamala, Ravi; Agarwal, Neha; Kumar, Pramod; Sundaram, Parthiban

    2004-01-01

    The HUMS automation system automates the design of HUMS architectures. The automated design process involves selection of solutions from a large space of designs as well as pure synthesis of designs. Hence the whole objective is to efficiently search for or synthesize designs or parts of designs in the database and to integrate them to form the entire system design. The automation system adopts two approaches in order to produce the designs: (a) Bottom-up approach and (b) Top down approach. Both the approaches are endowed with a Suite of quantitative and quantitative techniques that enable a) the selection of matching component instances, b) the determination of design parameters, c) the evaluation of candidate designs at component-level and at system-level, d) the performance of cost-benefit analyses, e) the performance of trade-off analyses, etc. In short, the automation system attempts to capitalize on the knowledge developed from years of experience in engineering, system design and operation of the HUMS systems in order to economically produce the most optimal and domain-specific designs.

  7. Integration of site-specific health information: Agency for Toxic Substances and Disease Registry health assessments

    SciTech Connect

    Lesperance, A.M.; Siegel, M.R.

    1990-12-01

    The Agency for Toxic Substances and Disease Registry is required to conduct a health assessment of any site that is listed on or proposed for the US Environmental Protection Agency's National Priorities List. Sixteen US Department of Energy (DOE) sites currently fall into this category. Health assessments contain a qualitative description of impacts to public health and the environment from hazardous waste sites, as well as recommendations for actions to mitigate or eliminate risk. Because these recommendations may have major impacts on compliance activities at DOE facilities, the health assessments are an important source of information for the monitoring activities of DOE's Office of Environmental Compliance (OEC). This report provides an overview of the activities involved in preparing the health assessment, its role in environmental management, and its key elements.

  8. Design specifications for manufacturability of MCM-C multichip modules

    SciTech Connect

    Allen, C.; Blazek, R.; Desch, J.; Elarton, J.; Kautz, D.; Markley, D.; Morgenstern, H.; Stewart, R.; Warner, L.

    1995-06-01

    The scope of this document is to establish design guidelines for electronic circuitry packaged as multichip modules of the ceramic substrate variety, although many of these guidelines are applicable to other types of multichip modules. The guidelines begin with prerequisite information which must be developed between customer and designer of the multichip module. The core of the guidelines focuses on the many considerations that must be addressed during the multichip module design. The guidelines conclude with the resulting deliverables from the design which satisfy customer requirements and/or support the multichip module fabrication and testing processes. Considerable supporting information, checklists, and design constraints are captured in specific appendices and used as reference information in the main body text. Finally some real examples of multichip module design are presented.

  9. Force limit specifications vs. design limit loads in vibration testing

    NASA Technical Reports Server (NTRS)

    Chang, K. Y.

    2000-01-01

    The purpose of the work presented herein is to discuss the results of force limit notching during vibration testing with respect to the traditional limit load design criteria. By using a single-degree-of-freedom (SDOF) system approach, this work shows that with an appropriate force specification the notched response due to force limiting will result in loads comparable with the structural design limit criteria.

  10. Specification, Design, and Analysis of Advanced HUMS Architectures

    NASA Technical Reports Server (NTRS)

    Mukkamala, Ravi

    2004-01-01

    During the two-year project period, we have worked on several aspects of domain-specific architectures for HUMS. In particular, we looked at using scenario-based approach for the design and designed a language for describing such architectures. The language is now being used in all aspects of our HUMS design. In particular, we have made contributions in the following areas. 1) We have employed scenarios in the development of HUMS in three main areas. They are: (a) To improve reusability by using scenarios as a library indexing tool and as a domain analysis tool; (b) To improve maintainability by recording design rationales from two perspectives - problem domain and solution domain; (c) To evaluate the software architecture. 2) We have defined a new architectural language called HADL or HUMS Architectural Definition Language. It is a customized version of xArch/xADL. It is based on XML and, hence, is easily portable from domain to domain, application to application, and machine to machine. Specifications written in HADL can be easily read and parsed using the currently available XML parsers. Thus, there is no need to develop a plethora of software to support HADL. 3) We have developed an automated design process that involves two main techniques: (a) Selection of solutions from a large space of designs; (b) Synthesis of designs. However, the automation process is not an absolute Artificial Intelligence (AI) approach though it uses a knowledge-based system that epitomizes a specific HUMS domain. The process uses a database of solutions as an aid to solve the problems rather than creating a new design in the literal sense. Since searching is adopted as the main technique, the challenges involved are: (a) To minimize the effort in searching the database where a very large number of possibilities exist; (b) To develop representations that could conveniently allow us to depict design knowledge evolved over many years; (c) To capture the required information that aid the

  11. NASA software specification and evaluation system design, part 2

    NASA Technical Reports Server (NTRS)

    1976-01-01

    A survey and analysis of the existing methods, tools and techniques employed in the development of software are presented along with recommendations for the construction of reliable software. Functional designs for software specification language, and the data base verifier are presented.

  12. Specifications for the Design of the School Site.

    ERIC Educational Resources Information Center

    Brubaker, C. William

    1986-01-01

    The school site design is now seen as part of community planning. Recreational, cultural, social, and educational facilities for adults are included in educational specifications. Three illustrations and two photographs demonstrated attractive school sites. Site area requirements for a new high school in Santa Fe, New Mexico are summarized. (MLF)

  13. 34 CFR 403.100 - What are the requirements for designating a State corrections educational agency to administer...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... corrections educational agency to administer the Programs for Criminal Offenders? 403.100 Section 403.100... ADULT EDUCATION, DEPARTMENT OF EDUCATION STATE VOCATIONAL AND APPLIED TECHNOLOGY EDUCATION PROGRAM What... § 403.100 What are the requirements for designating a State corrections educational agency to...

  14. 77 FR 52744 - Food and Drug Administration/European Medicines Agency Orphan Product Designation and Grant Workshop

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-30

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration Food and Drug Administration/European Medicines Agency Orphan Product Designation and Grant Workshop AGENCY: Food and Drug Administration, HHS. ACTION: Notice of meeting. The Food and Drug Administration's...

  15. 78 FR 51732 - The Food and Drug Administration/European Medicines Agency Orphan Product Designation and Grant...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-21

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration The Food and Drug Administration/European Medicines Agency Orphan Product Designation and Grant Public Workshop AGENCY: Food and Drug Administration, HHS. ACTION: Notice of public workshop. The Food and...

  16. A design guide and specification for small explosive containment structures

    SciTech Connect

    Marchand, K.A.; Cox, P.A.; Polcyn, M.A.

    1994-12-01

    The design of structural containments for testing small explosive devices requires the designer to consider the various aspects of the explosive loading, i.e., shock and gas or quasistatic pressure. Additionally, if the explosive charge has the potential of producing damaging fragments, provisions must be made to arrest the fragments. This may require that the explosive be packed in a fragment attenuating material, which also will affect the loads predicted for containment response. Material also may be added just to attenuate shock, in the absence of fragments. Three charge weights are used in the design. The actual charge is used to determine a design fragment. Blast loads are determined for a {open_quotes}design charge{close_quotes}, defined as 125% of the operational charge in the explosive device. No yielding is permitted at the design charge weight. Blast loads are also determined for an over-charge, defined as 200% of the operational charge in the explosive device. Yielding, but no failure, is permitted at this over-charge. This guide emphasizes the calculation of loads and fragments for which the containment must be designed. The designer has the option of using simplified or complex design-analysis methods. Examples in the guide use readily available single degree-of-freedom (sdof) methods, plus static methods for equivalent dynamic loads. These are the common methods for blast resistant design. Some discussion of more complex methods is included. Generally, the designer who chooses more complex methods must be fully knowledgeable in their use and limitations. Finally, newly fabricated containments initially must be proof tested to 125% of the operational load and then inspected at regular intervals. This specification provides guidance for design, proof testing, and inspection of small explosive containment structures.

  17. A Generic Method for Design of Oligomer-Specific Antibodies

    PubMed Central

    Brännström, Kristoffer; Lindhagen-Persson, Malin; Gharibyan, Anna L.; Iakovleva, Irina; Vestling, Monika; Sellin, Mikael E.; Brännström, Thomas; Morozova-Roche, Ludmilla; Forsgren, Lars; Olofsson, Anders

    2014-01-01

    Antibodies that preferentially and specifically target pathological oligomeric protein and peptide assemblies, as opposed to their monomeric and amyloid counterparts, provide therapeutic and diagnostic opportunities for protein misfolding diseases. Unfortunately, the molecular properties associated with oligomer-specific antibodies are not well understood, and this limits targeted design and development. We present here a generic method that enables the design and optimisation of oligomer-specific antibodies. The method takes a two-step approach where discrimination between oligomers and fibrils is first accomplished through identification of cryptic epitopes exclusively buried within the structure of the fibrillar form. The second step discriminates between monomers and oligomers based on differences in avidity. We show here that a simple divalent mode of interaction, as within e.g. the IgG isotype, can increase the binding strength of the antibody up to 1500 times compared to its monovalent counterpart. We expose how the ability to bind oligomers is affected by the monovalent affinity and the turnover rate of the binding and, importantly, also how oligomer specificity is only valid within a specific concentration range. We provide an example of the method by creating and characterising a spectrum of different monoclonal antibodies against both the Aβ peptide and α-synuclein that are associated with Alzheimer's and Parkinson's diseases, respectively. The approach is however generic, does not require identification of oligomer-specific architectures, and is, in essence, applicable to all polypeptides that form oligomeric and fibrillar assemblies. PMID:24618582

  18. Specific issues of the design for the elderly

    NASA Astrophysics Data System (ADS)

    Sebesi, S. B.; Groza, H. L.; Ianoşi, A.; Dimitrova, A.; Mândru, D.

    2016-08-01

    The actual demographic studies show that number of the elderly people is increasing constantly. Considering their motor, sensorial and cognitive constrains and restrictions, a new field of Assistive Technology is developing, focussed on the design and development of a wide range of devices, apparatus, equipment and systems dedicated to their independent and safe life. In this paper, a systematisation of existing gero-technical systems is proposed, emphasising the today trends in this field. The specific issues of designing this kind of products are identified and analysed. Two approaches of the authors are finally presented: wearable suits for aging and disabilities simulation and tele-monitoring of the elderly.

  19. Space tug thermal control. [design criteria and specifications

    NASA Technical Reports Server (NTRS)

    1974-01-01

    It was determined that space tug will require the capability to perform its mission within a broad range of thermal environments with currently planned mission durations of up to seven days, so an investigation was conducted to define a thermal design for the forward and intertank compartments and fuel cell heat rejection system that satisfies tug requirements for low inclination geosynchronous deploy and retrieve missions. Passive concepts were demonstrated analytically for both the forward and intertank compartments, and a worst case external heating environment was determined for use during the study. The thermal control system specifications and designs which resulted from the research are shown.

  20. High specific energy, high capacity nickel-hydrogen cell design

    NASA Technical Reports Server (NTRS)

    Wheeler, James R.

    1993-01-01

    A 3.5 inch rabbit-ear-terminal nickel-hydrogen cell has been designed and tested to deliver high capacity at a C/1.5 discharge rate. Its specific energy yield of 60.6 wh/kg is believed to be the highest yet achieved in a slurry-process nickel-hydrogen cell, and its 10 C capacity of 113.9 AH the highest capacity yet made at a discharge rate this high in the 3.5 inch diameter size. The cell also demonstrated a pulse capability of 180 amps for 20 seconds. Specific cell parameters, performance, and future test plans are described.

  1. 41 CFR 102-76.20 - What issues must Federal agencies consider in providing site planning and landscape design services?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design and Construction § 102-76.20... 41 Public Contracts and Property Management 3 2014-01-01 2014-01-01 false What issues must Federal agencies consider in providing site planning and landscape design services? 102-76.20 Section...

  2. 41 CFR 102-76.30 - What seismic safety standards must Federal agencies follow in the design and construction of...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... (Continued) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design and Construction... 41 Public Contracts and Property Management 3 2012-01-01 2012-01-01 false What seismic safety standards must Federal agencies follow in the design and construction of Federal facilities?...

  3. 41 CFR 102-76.20 - What issues must Federal agencies consider in providing site planning and landscape design services?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design and Construction § 102-76.20... 41 Public Contracts and Property Management 3 2011-01-01 2011-01-01 false What issues must Federal agencies consider in providing site planning and landscape design services? 102-76.20 Section...

  4. 41 CFR 102-76.30 - What seismic safety standards must Federal agencies follow in the design and construction of...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (Continued) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design and Construction... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false What seismic safety standards must Federal agencies follow in the design and construction of Federal facilities?...

  5. 41 CFR 102-76.30 - What seismic safety standards must Federal agencies follow in the design and construction of...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... (Continued) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design and Construction... 41 Public Contracts and Property Management 3 2011-01-01 2011-01-01 false What seismic safety standards must Federal agencies follow in the design and construction of Federal facilities?...

  6. 41 CFR 102-76.55 - What sustainable development principles must Federal agencies apply to the siting, design, and...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Regulations System (Continued) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design... 41 Public Contracts and Property Management 3 2014-01-01 2014-01-01 false What sustainable development principles must Federal agencies apply to the siting, design, and construction of new...

  7. 41 CFR 102-76.30 - What seismic safety standards must Federal agencies follow in the design and construction of...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (Continued) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design and Construction... 41 Public Contracts and Property Management 3 2013-07-01 2013-07-01 false What seismic safety standards must Federal agencies follow in the design and construction of Federal facilities?...

  8. 41 CFR 102-76.20 - What issues must Federal agencies consider in providing site planning and landscape design services?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design and Construction § 102-76.20... 41 Public Contracts and Property Management 3 2012-01-01 2012-01-01 false What issues must Federal agencies consider in providing site planning and landscape design services? 102-76.20 Section...

  9. 41 CFR 102-76.55 - What sustainable development principles must Federal agencies apply to the siting, design, and...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Regulations System (Continued) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design... 41 Public Contracts and Property Management 3 2013-07-01 2013-07-01 false What sustainable development principles must Federal agencies apply to the siting, design, and construction of new...

  10. 41 CFR 102-76.20 - What issues must Federal agencies consider in providing site planning and landscape design services?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design and Construction § 102-76.20... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false What issues must Federal agencies consider in providing site planning and landscape design services? 102-76.20 Section...

  11. 41 CFR 102-76.30 - What seismic safety standards must Federal agencies follow in the design and construction of...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... (Continued) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design and Construction... 41 Public Contracts and Property Management 3 2014-01-01 2014-01-01 false What seismic safety standards must Federal agencies follow in the design and construction of Federal facilities?...

  12. 41 CFR 102-76.55 - What sustainable development principles must Federal agencies apply to the siting, design, and...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Regulations System (Continued) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design... 41 Public Contracts and Property Management 3 2012-01-01 2012-01-01 false What sustainable development principles must Federal agencies apply to the siting, design, and construction of new...

  13. 41 CFR 102-76.55 - What sustainable development principles must Federal agencies apply to the siting, design, and...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Regulations System (Continued) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION Design... 41 Public Contracts and Property Management 3 2011-01-01 2011-01-01 false What sustainable development principles must Federal agencies apply to the siting, design, and construction of new...

  14. Precise Specification of Design Pattern Structure and Behaviour

    NASA Astrophysics Data System (ADS)

    Sterritt, Ashley; Clarke, Siobhán; Cahill, Vinny

    Applying design patterns while developing a software system can improve its non-functional properties, such as extensibility and loose coupling. Precise specification of structure and behaviour communicates the invariants imposed by a pattern on a conforming implementation and enables formal software verification. Many existing design-pattern specification languages (DPSLs) focus on class structure alone, while those that do address behaviour suffer from a lack of expressiveness and/or imprecise semantics. In particular, in a review of existing work, three invariant categories were found to be inexpressible in state-of-the-art DPSLs: dependency, object state and data-structure. This paper presents Alas: a precise specification language that supports design-pattern descriptions including these invariant categories. The language is based on UML Class and Sequence diagrams with modified syntax and semantics. In this paper, the meaning of the presented invariants is formalized and relevant ambiguities in the UML Standard are clarified. We have evaluated Alas by specifying the widely-used Gang of Four pattern catalog and identified patterns that benefitted from the added expressiveness and semantics of Alas.

  15. Dye laser amplifier including a specifically designed diffuser assembly

    DOEpatents

    Davin, James; Johnston, James P.

    1992-01-01

    A large (high flow rate) dye laser amplifier in which a continuous replened supply of dye is excited by a first light beam, specifically a copper vapor laser beam, in order to amplify the intensity of a second different light beam, specifically a dye beam, passing through the dye is disclosed herein. This amplifier includes a dye cell defining a dye chamber through which a continuous stream of dye is caused to pass at a relatively high flow rate and a specifically designed diffuser assembly for slowing down the flow of dye while, at the same time, assuring that as the dye stream flows through the diffuser assembly it does so in a stable manner.

  16. 41 CFR 302-9.170 - Under what specific conditions may my agency authorize transportation of a POV to my post of duty...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... conditions may my agency authorize transportation of a POV to my post of duty subsequent to the time of my assignment to that post? 302-9.170 Section 302-9.170 Public Contracts and Property Management Federal Travel... Subsequent to the Time of Assignment § 302-9.170 Under what specific conditions may my agency...

  17. 41 CFR 302-9.170 - Under what specific conditions may my agency authorize transportation of a POV to my post of duty...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... conditions may my agency authorize transportation of a POV to my post of duty subsequent to the time of my assignment to that post? 302-9.170 Section 302-9.170 Public Contracts and Property Management Federal Travel... Subsequent to the Time of Assignment § 302-9.170 Under what specific conditions may my agency...

  18. 41 CFR 302-9.170 - Under what specific conditions may my agency authorize transportation of a POV to my post of duty...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... conditions may my agency authorize transportation of a POV to my post of duty subsequent to the time of my assignment to that post? 302-9.170 Section 302-9.170 Public Contracts and Property Management Federal Travel... the Time of Assignment § 302-9.170 Under what specific conditions may my agency...

  19. 41 CFR 302-9.170 - Under what specific conditions may my agency authorize transportation of a POV to my post of duty...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... conditions may my agency authorize transportation of a POV to my post of duty subsequent to the time of my assignment to that post? 302-9.170 Section 302-9.170 Public Contracts and Property Management Federal Travel... Subsequent to the Time of Assignment § 302-9.170 Under what specific conditions may my agency...

  20. 41 CFR 302-9.170 - Under what specific conditions may my agency authorize transportation of a POV to my post of duty...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... conditions may my agency authorize transportation of a POV to my post of duty subsequent to the time of my assignment to that post? 302-9.170 Section 302-9.170 Public Contracts and Property Management Federal Travel... the Time of Assignment § 302-9.170 Under what specific conditions may my agency...

  1. Modular Integrated Stackable Layers (MISL) 1.1 Design Specification. Design Guideline Document

    NASA Technical Reports Server (NTRS)

    Yim, Hester J.

    2012-01-01

    This document establishes the design guideline of the Modular Instrumentation Data Acquisition (MI-DAQ) system in utilization of several designs available in EV. The MI- DAQ provides the options to the customers depending on their system requirements i.e. a 28V interface power supply, a low power battery operated system, a low power microcontroller, a higher performance microcontroller, a USB interface, a Ethernet interface, a wireless communication, various sensor interfaces, etc. Depending on customer's requirements, the each functional board can be stacked up from a bottom level of power supply to a higher level of stack to provide user interfaces. The stack up of boards are accomplished by a predefined and standardized power bus and data bus connections which are included in this document along with other physical and electrical guidelines. This guideline also provides information for a new design options. This specification is the product of a collaboration between NASA/JSC/EV and Texas A&M University. The goal of the collaboration is to open source the specification and allow outside entities to design, build, and market modules that are compatible with the specification. NASA has designed and is using numerous modules that are compatible to this specification. A limited number of these modules will also be released as open source designs to support the collaboration. The released designs are listed in the Applicable Documents.

  2. High specific energy, high capacity nickel-hydrogen cell design

    NASA Technical Reports Server (NTRS)

    Wheeler, James R.

    1993-01-01

    A 3.5 inch rabbit-ear-terminal nickel-hydrogen cell was designed and tested to deliver high capacity at steady discharge rates up to and including a C rate. Its specific energy yield of 60.6 wh/kg is believed to be the highest yet achieved in a slurry-process nickel-hydrogen cell, and its 10 C capacity of 113.9 AH the highest capacity yet of any type in a 3.5 inch diameter size. The cell also demonstrated a pulse capability of 180 amps for 20 seconds. Specific cell parameters and performance are described. Also covered is an episode of capacity fading due to electrode swelling and its successful recovery by means of additional activation procedures.

  3. Advanced Wet Tantalum Capacitors: Design, Specifications and Performance

    NASA Technical Reports Server (NTRS)

    Teverovsky, Alexander

    2017-01-01

    Insertion of new types of commercial, high volumetric efficiency wet tantalum capacitors in space systems requires reassessment of the existing quality assurance approaches that have been developed for capacitors manufactured to MIL-PRF-39006 requirements. The specifics of wet electrolytic capacitors is that leakage currents flowing through electrolyte can cause gas generation resulting in building up of internal gas pressure and rupture of the case. The risk associated with excessive leakage currents and increased pressure is greater for high value advanced wet tantalum capacitors, but it has not been properly evaluated yet. This presentation gives a review of specifics of the design, performance, and potential reliability risks associated with advanced wet tantalum capacitors. Problems related to setting adequate requirements for DPA, leakage currents, hermeticity, stability at low and high temperatures, ripple currents for parts operating in vacuum, and random vibration testing are discussed. Recommendations for screening and qualification to reduce risks of failures have been suggested.

  4. Advanced Wet Tantalum Capacitors: Design, Specifications and Performance

    NASA Technical Reports Server (NTRS)

    Teverovsky, Alexander

    2016-01-01

    Insertion of new types of commercial, high volumetric efficiency wet tantalum capacitors in space systems requires reassessment of the existing quality assurance approaches that have been developed for capacitors manufactured to MIL-PRF-39006 requirements. The specifics of wet electrolytic capacitors is that leakage currents flowing through electrolyte can cause gas generation resulting in building up of internal gas pressure and rupture of the case. The risk associated with excessive leakage currents and increased pressure is greater for high value advanced wet tantalum capacitors, but it has not been properly evaluated yet. This presentation gives a review of specifics of the design, performance, and potential reliability risks associated with advanced wet tantalum capacitors. Problems related to setting adequate requirements for DPA, leakage currents, hermeticity, stability at low and high temperatures, ripple currents for parts operating in vacuum, and random vibration testing are discussed. Recommendations for screening and qualification to reduce risks of failures have been suggested.

  5. Towards the Design of a Patient-Specific Virtual Tumour

    PubMed Central

    Caraguel, Flavien; Lesart, Anne-Cécile; Estève, François; van der Sanden, Boudewijn

    2016-01-01

    The design of a patient-specific virtual tumour is an important step towards Personalized Medicine. However this requires to capture the description of many key events of tumour development, including angiogenesis, matrix remodelling, hypoxia, and cell state heterogeneity that will all influence the tumour growth kinetics and degree of tumour invasiveness. To that end, an integrated hybrid and multiscale approach has been developed based on data acquired on a preclinical mouse model as a proof of concept. Fluorescence imaging is exploited to build case-specific virtual tumours. Numerical simulations show that the virtual tumour matches the characteristics and spatiotemporal evolution of its real counterpart. We achieved this by combining image analysis and physiological modelling to accurately described the evolution of different tumour cases over a month. The development of such models is essential since a dedicated virtual tumour would be the perfect tool to identify the optimum therapeutic strategies that would make Personalized Medicine truly reachable and achievable. PMID:28096895

  6. Designing pressure garments capable of exerting specific pressures on limbs.

    PubMed

    Macintyre, Lisa

    2007-08-01

    Pressure garments have been used prophylactically and to treat hypertrophic scars, resulting from serious burns, since the early 1970s. They are custom-made from elastic fabrics by commercial producers and hospital staff. However, no clear scientifically established method has ever been published for their design and manufacture. Previous work [2] identified the most commonly used fabrics and construction methods for the production of pressure garments by hospital staff in UK burn units. These methods were evaluated by measuring pressures delivered to both cylinder models and to human limbs using I-scan pressure sensors. A new calibration method was developed for the I-scan system to enable measurement of low interface pressures to an accuracy of +/-2.5 mmHg. The effects of cylinder/limb circumference and pressure garment design on the pressures exerted were established. These measurements confirm the limitations of current pressure garment construction methods used in UK hospitals. A new method for designing pressure garments that will exert specific known pressures is proposed and evaluated for human thighs. Evaluation of the proposed design method is ongoing for other body parts.

  7. Compressive imaging system design using task-specific information.

    PubMed

    Ashok, Amit; Baheti, Pawan K; Neifeld, Mark A

    2008-09-01

    We present a task-specific information (TSI) based framework for designing compressive imaging (CI) systems. The task of target detection is chosen to demonstrate the performance of the optimized CI system designs relative to a conventional imager. In our optimization framework, we first select a projection basis and then find the associated optimal photon-allocation vector in the presence of a total photon-count constraint. Several projection bases, including principal components (PC), independent components, generalized matched-filter, and generalized Fisher discriminant (GFD) are considered for candidate CI systems, and their respective performance is analyzed for the target-detection task. We find that the TSI-optimized CI system design based on a GFD projection basis outperforms all other candidate CI system designs as well as the conventional imager. The GFD-based compressive imager yields a TSI of 0.9841 bits (out of a maximum possible 1 bit for the detection task), which is nearly ten times the 0.0979 bits achieved by the conventional imager at a signal-to-noise ratio of 5.0. We also discuss the relation between the information-theoretic TSI metric and a conventional statistical metric like probability of error in the context of the target-detection problem. It is shown that the TSI can be used to derive an upper bound on the probability of error that can be attained by any detection algorithm.

  8. Integrating Cloud-Computing-Specific Model into Aircraft Design

    NASA Astrophysics Data System (ADS)

    Zhimin, Tian; Qi, Lin; Guangwen, Yang

    Cloud Computing is becoming increasingly relevant, as it will enable companies involved in spreading this technology to open the door to Web 3.0. In the paper, the new categories of services introduced will slowly replace many types of computational resources currently used. In this perspective, grid computing, the basic element for the large scale supply of cloud services, will play a fundamental role in defining how those services will be provided. The paper tries to integrate cloud computing specific model into aircraft design. This work has acquired good results in sharing licenses of large scale and expensive software, such as CFD (Computational Fluid Dynamics), UG, CATIA, and so on.

  9. Design and Evaluation of Tumor-Specific Dendrimer Epigenetic Therapeutics.

    PubMed

    Zong, Hong; Shah, Dhavan; Selwa, Katherine; Tsuchida, Ryan E; Rattan, Rahul; Mohan, Jay; Stein, Adam B; Otis, James B; Goonewardena, Sascha N

    2015-06-01

    Histone deacetylase inhibitors (HDACi) are promising therapeutics for cancer. HDACi alter the epigenetic state of tumors and provide a unique approach to treat cancer. Although studies with HDACi have shown promise in some cancers, variable efficacy and off-target effects have limited their use. To overcome some of the challenges of traditional HDACi, we sought to use a tumor-specific dendrimer scaffold to deliver HDACi directly to cancer cells. Here we report the design and evaluation of tumor-specific dendrimer-HDACi conjugates. The HDACi was conjugated to the dendrimer using an ester linkage through its hydroxamic acid group, inactivating the HDACi until it is released from the dendrimer. Using a cancer cell model, we demonstrate the functionality of the tumor-specific dendrimer-HDACi conjugates. Furthermore, we demonstrate that unlike traditional HDACi, dendrimer-HDACi conjugates do not affect tumor-associated macrophages, a recently recognized mechanism through which drug resistance emerges. We anticipate that this new class of cell-specific epigenetic therapeutics will have tremendous potential in the treatment of cancer.

  10. 28 CFR 81.2 - Submission of reports; designation of agencies to receive reports of child abuse.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Submission of reports; designation of agencies to receive reports of child abuse. 81.2 Section 81.2 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.2 Submission...

  11. 28 CFR 81.2 - Submission of reports; designation of agencies to receive reports of child abuse.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Submission of reports; designation of agencies to receive reports of child abuse. 81.2 Section 81.2 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.2 Submission...

  12. 28 CFR 81.2 - Submission of reports; designation of agencies to receive reports of child abuse.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Submission of reports; designation of agencies to receive reports of child abuse. 81.2 Section 81.2 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.2 Submission...

  13. 28 CFR 81.2 - Submission of reports; designation of agencies to receive reports of child abuse.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Submission of reports; designation of agencies to receive reports of child abuse. 81.2 Section 81.2 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.2 Submission...

  14. 28 CFR 81.2 - Submission of reports; designation of agencies to receive reports of child abuse.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Submission of reports; designation of agencies to receive reports of child abuse. 81.2 Section 81.2 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) CHILD ABUSE AND CHILD PORNOGRAPHY REPORTING DESIGNATIONS AND PROCEDURES § 81.2 Submission...

  15. 5 CFR 5501.102 - Designation of HHS components as separate agencies.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... remainder of HHS: (1) Administration on Aging; (2) Administration for Children and Families: (3) Agency for Healthcare Research and Quality; (4) Agency for Toxic Substances and Disease Registry; (5) Centers...

  16. 5 CFR 5501.102 - Designation of HHS components as separate agencies.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... remainder of HHS: (1) Administration on Aging; (2) Administration for Children and Families: (3) Agency for Healthcare Research and Quality; (4) Agency for Toxic Substances and Disease Registry; (5) Centers...

  17. 41 CFR 102-74.340 - Who is responsible for monitoring and controlling areas designated for smoking by an agency head...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... monitoring and controlling areas designated for smoking by an agency head and for identifying those areas... MANAGEMENT Facility Management Smoking § 102-74.340 Who is responsible for monitoring and controlling areas designated for smoking by an agency head and for identifying those areas with proper signage? Agency...

  18. 41 CFR 102-74.340 - Who is responsible for monitoring and controlling areas designated for smoking by an agency head...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... monitoring and controlling areas designated for smoking by an agency head and for identifying those areas... MANAGEMENT Facility Management Smoking § 102-74.340 Who is responsible for monitoring and controlling areas designated for smoking by an agency head and for identifying those areas with proper signage? Agency...

  19. 41 CFR 102-74.340 - Who is responsible for monitoring and controlling areas designated for smoking by an agency head...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... monitoring and controlling areas designated for smoking by an agency head and for identifying those areas... MANAGEMENT Facility Management Smoking § 102-74.340 Who is responsible for monitoring and controlling areas designated for smoking by an agency head and for identifying those areas with proper signage? Agency...

  20. 41 CFR 102-74.340 - Who is responsible for monitoring and controlling areas designated for smoking by an agency head...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... monitoring and controlling areas designated for smoking by an agency head and for identifying those areas... MANAGEMENT Facility Management Smoking § 102-74.340 Who is responsible for monitoring and controlling areas designated for smoking by an agency head and for identifying those areas with proper signage? Agency...

  1. 41 CFR 102-74.340 - Who is responsible for monitoring and controlling areas designated for smoking by an agency head...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... monitoring and controlling areas designated for smoking by an agency head and for identifying those areas... MANAGEMENT Facility Management Smoking § 102-74.340 Who is responsible for monitoring and controlling areas designated for smoking by an agency head and for identifying those areas with proper signage? Agency...

  2. An integrated in silico approach to design specific inhibitors targeting human poly(a)-specific ribonuclease.

    PubMed

    Vlachakis, Dimitrios; Pavlopoulou, Athanasia; Tsiliki, Georgia; Komiotis, Dimitri; Stathopoulos, Constantinos; Balatsos, Nikolaos A A; Kossida, Sophia

    2012-01-01

    Poly(A)-specific ribonuclease (PARN) is an exoribonuclease/deadenylase that degrades 3'-end poly(A) tails in almost all eukaryotic organisms. Much of the biochemical and structural information on PARN comes from the human enzyme. However, the existence of PARN all along the eukaryotic evolutionary ladder requires further and thorough investigation. Although the complete structure of the full-length human PARN, as well as several aspects of the catalytic mechanism still remain elusive, many previous studies indicate that PARN can be used as potent and promising anti-cancer target. In the present study, we attempt to complement the existing structural information on PARN with in-depth bioinformatics analyses, in order to get a hologram of the molecular evolution of PARNs active site. In an effort to draw an outline, which allows specific drug design targeting PARN, an unequivocally specific platform was designed for the development of selective modulators focusing on the unique structural and catalytic features of the enzyme. Extensive phylogenetic analysis based on all the publicly available genomes indicated a broad distribution for PARN across eukaryotic species and revealed structurally important amino acids which could be assigned as potentially strong contributors to the regulation of the catalytic mechanism of PARN. Based on the above, we propose a comprehensive in silico model for the PARN's catalytic mechanism and moreover, we developed a 3D pharmacophore model, which was subsequently used for the introduction of DNP-poly(A) amphipathic substrate analog as a potential inhibitor of PARN. Indeed, biochemical analysis revealed that DNP-poly(A) inhibits PARN competitively. Our approach provides an efficient integrated platform for the rational design of pharmacophore models as well as novel modulators of PARN with therapeutic potential.

  3. Cloning a neutral protease of Clostridium histolyticum, determining its substrate specificity, and designing a specific substrate.

    PubMed

    Maeda, Hiroshi; Nakagawa, Kanako; Murayama, Kazutaka; Goto, Masafumi; Watanabe, Kimiko; Takeuchi, Michio; Yamagata, Youhei

    2015-12-01

    Islet transplantation is a prospective treatment for restoring normoglycemia in patients with type 1 diabetes. Islet isolation from pancreases by decomposition with proteolytic enzymes is necessary for transplantation. Two collagenases, collagenase class I (ColG) and collagenase class II (ColH), from Clostridium histolyticum have been used for islet isolation. Neutral proteases have been added to the collagenases for human islet isolation. A neutral protease from C. histolyticum (NP) and thermolysin from Bacillus thermoproteolyicus has been used for the purpose. Thermolysin is an extensively studied enzyme, but NP is not well known. We therefore cloned the gene encoding NP and constructed a Bacillus subtilis overexpression strain. The expressed enzyme was purified, and its substrate specificity was examined. We observed that the substrate specificity of NP was higher than that of thermolysin, and that the protein digestion activities of NP, as determined by colorimetric methods, were lower than those of thermolysin. It seems that decomposition using NP does not negatively affect islets during islet preparation from pancreases. Furthermore, we designed a novel substrate that allows the measurement of NP activity specifically in the enzyme mixture for islet preparation and the culture broth of C. histolyticum. The activity of NP can also be monitored during islet isolation. We hope the purified enzyme and this specific substrate contribute to the optimization of islet isolation from pancreases and that it leads to the success of islet transplantation and the improvement of the quality of life (QOL) for diabetic patients.

  4. A drug-specific nanocarrier design for efficient anticancer therapy

    PubMed Central

    Shi, Changying; Guo, Dandan; Xiao, Kai; Wang, Xu; Wang, Lili; Luo, Juntao

    2015-01-01

    The drug-loading properties of nanocarriers depend on the chemical structures and properties of their building blocks. Here, we customize telodendrimers (linear-dendritic copolymer) to design a nanocarrier with improved in vivo drug delivery characteristics. We do a virtual screen of a library of small molecules to identify the optimal building blocks for precise telodendrimer synthesis using peptide chemistry. With rationally designed telodendrimer architectures, we then optimize the drug binding affinity of a nanocarrier by introducing an optimal drug-binding molecule (DBM) without sacrificing the stability of the nanocarrier. To validate the computational predictions, we synthesize a series of nanocarriers and evaluate systematically for doxorubicin delivery. Rhein-containing nanocarriers have sustained drug release, prolonged circulation, increased tolerated dose, reduced toxicity, effective tumor targeting and superior anticancer effects owing to favourable doxorubicin-binding affinity and improved nanoparticle stability. This study demonstrates the feasibility and versatility of the de novo design of telodendrimer nanocarriers for specific drug molecules, which is a promising approach to transform nanocarrier development for drug delivery. PMID:26158623

  5. A drug-specific nanocarrier design for efficient anticancer therapy

    NASA Astrophysics Data System (ADS)

    Shi, Changying; Guo, Dandan; Xiao, Kai; Wang, Xu; Wang, Lili; Luo, Juntao

    2015-07-01

    The drug-loading properties of nanocarriers depend on the chemical structures and properties of their building blocks. Here we customize telodendrimers (linear dendritic copolymer) to design a nanocarrier with improved in vivo drug delivery characteristics. We do a virtual screen of a library of small molecules to identify the optimal building blocks for precise telodendrimer synthesis using peptide chemistry. With rationally designed telodendrimer architectures, we then optimize the drug-binding affinity of a nanocarrier by introducing an optimal drug-binding molecule (DBM) without sacrificing the stability of the nanocarrier. To validate the computational predictions, we synthesize a series of nanocarriers and evaluate systematically for doxorubicin delivery. Rhein-containing nanocarriers have sustained drug release, prolonged circulation, increased tolerated dose, reduced toxicity, effective tumour targeting and superior anticancer effects owing to favourable doxorubicin-binding affinity and improved nanoparticle stability. This study demonstrates the feasibility and versatility of the de novo design of telodendrimer nanocarriers for specific drug molecules, which is a promising approach to transform nanocarrier development for drug delivery.

  6. Environmental Protection Agency's municipal-solid-waste landfill-liner design criteria

    SciTech Connect

    Landreth, R.E.

    1992-01-01

    The soon to be published non-hazardous land disposal regulation, RCRA sub title D (40 CFR Parts 257 and 258) must achieve three objectives. The first is to be protective of human health and the environment, second to be flexible so as not to stifle innovative designs, and third to allow individual states the latitude to develop state specific regulations. The containment systems (liners) currently under consideration will achieve these three goals. The paper will briefly discuss the approach to the liner design. Additional information will be forth coming as final decisions are made.

  7. An overview of field specific designs of microbial EOR

    SciTech Connect

    Robertson, E.P.; Bala, G.A.; Fox, S.L.; Jackson, J.D.; Thomas, C.P.

    1995-12-01

    The selection and design of a microbial enhanced oil recovery (MEOR) process for application in a specific field involves geological, reservoir, and biological characterization. Microbially mediated oil recovery mechanisms (biogenic gas, biopolymers, and biosurfactants) are defined by the types of microorganisms used. The engineering and biological character of a given reservoir must be understood to correctly select a microbial system to enhance oil recovery. The objective of this paper is to discuss the methods used to evaluate three fields with distinct characteristics and production problems for the applicability of MEOR technology. Reservoir characteristics and laboratory results indicated that MEOR would not be applicable in two of the three fields considered. The development of a microbial oil recovery process for the third field appeared promising. Development of a bacterial consortium capable of producing the desired metabolites was initiated and field isolates were characterized.

  8. Novel Designs for Application Specific MEMS Pressure Sensors

    PubMed Central

    Fragiacomo, Giulio; Reck, Kasper; Lorenzen, Lasse; Thomsen, Erik V.

    2010-01-01

    In the framework of developing innovative microfabricated pressure sensors, we present here three designs based on different readout principles, each one tailored for a specific application. A touch mode capacitive pressure sensor with high sensitivity (14 pF/bar), low temperature dependence and high capacitive output signal (more than 100 pF) is depicted. An optical pressure sensor intrinsically immune to electromagnetic interference, with large pressure range (0–350 bar) and a sensitivity of 1 pm/bar is presented. Finally, a resonating wireless pressure sensor power source free with a sensitivity of 650 KHz/mmHg is described. These sensors will be related with their applications in harsh environment, distributed systems and medical environment, respectively. For many aspects, commercially available sensors, which in vast majority are piezoresistive, are not suited for the applications proposed. PMID:22163425

  9. An overview of field-specific designs of microbial EOR

    SciTech Connect

    Robertson, E.P.; Bala, G.A.; Fox, S.L.; Jackson, J.D.; Thomas, C.P.

    1995-12-31

    The selection and design of an MEOR process for application in a specific field involves geological, reservoir, and biological characterization. Microbially mediated oil recovery mechanisms (bigenic gas, biopolymers, and biosurfactants) are defined by the types of microorganisms used. The engineering and biological character of a given reservoir must be understood to correctly select a microbial system to enhance oil recovery. This paper discusses the methods used to evaluate three fields with distinct characteristics and production problems for the applicability of MEOR would not be applicable in two of the three fields considered. The development of a microbial oil recovery process for the third field appeared promising. Development of a bacterial consortium capable of producing the desired metabolites was initiated, and field isolates were characterized.

  10. Geometric design of mechanical linkages for contact specifications

    NASA Astrophysics Data System (ADS)

    Robson, Nina Patarinsky

    2008-10-01

    This dissertation focuses on the kinematic synthesis of mechanical linkages in order to guide an end-effortor so that it maintains contact with specified objects in its workspace. Assuming the serial chain does not have full mobility in its workspace, the contact geometry is used to determine the dimensions of the serial chain. The approach to this problem, is to use the relative curvature of the contact of the end-effector with one or more objects to define velocity and acceleration specifications for its movement. This provides kinematic constraints that are used to synthesize the dimensions of the serial chain. The mathematical formulation of the geometric design problem, leads to systems of multivariable polynomial equations, which are solved exactly using sparse matrix resultants and polynomial homotopy methods. The results from this research yield planar RR and 4R linkages that match a specified contact geometry, spatial TS, parallel RRS and perpendicular RRS linkages that have a required acceleration specification. A new strategy for a robot recovery from actuator failures is demonstrated for the Mars Exploratory Rover Arm. In extending this work to spatial serial chains, a new method based on sparse matrix resultants was developed, which solves exact synthesis problems with acceleration constraints. Further the research builds on the theoretical concepts of contact relationships for spatial movement. The connection between kinematic synthesis and contact problems and its extension to spatial synthesis are developed in this dissertation for the first time and are new contributions. The results, which rely upon the use of surface curvature effects to reduce the number of fixtures needed to immobilize an object, find applications in robot grasping and part-fixturing. The recovery strategy, presented in this research is also a new concept. The recognition that it is possible to reconfigure a crippled robotic system to achieve mission critical tasks can guide

  11. DITDOS: A set of design specifications for distributed data inventories

    NASA Technical Reports Server (NTRS)

    King, T. A.; Walker, R. J.; Joy, S. P.

    1995-01-01

    The analysis of space science data often requires researchers to work with many different types of data. For instance, correlative analysis can require data from multiple instruments on a single spacecraft, multiple spacecraft, and ground-based data. Typically, data from each source are available in a different format and have been written on a different type of computer, and so much effort must be spent to read the data and convert it to the computer and format that the researchers use in their analysis. The large and ever-growing amount of data and the large investment by the scientific community in software that require a specific data format make using standard data formats impractical. A format-independent approach to accessing and analyzing disparate data is key to being able to deliver data to a diverse community in a timely fashion. The system in use at the Planetary Plasma Interactions (PPI) node of the NASA Planetary Data System (PDS) is based on the object-oriented Distributed Inventory Tracking and Data Ordering Specification (DITDOS), which describes data inventories in a storage independent way. The specifications have been designed to make it possible to build DITDOS compliant inventories that can exist on portable media such as CD-ROM's. The portable media can be moved within a system, or from system to system, and still be used without modification. Several applications have been developed to work with DITDOS compliant data holdings. One is a windows-based client/server application, which helps guide the user in the selection of data. A user can select a data base, then a data set, then a specific data file, and then either order the data and receive it immediately if it is online or request that it be brought online if it is not. A user can also view data by any of the supported methods. DITDOS makes it possible to use already existing applications for data-specific actions, and this is done whenever possible. Another application is a stand

  12. Should dialysis modalities be designed to remove specific uremic toxins?

    PubMed

    Baurmeister, Ulrich; Vienken, Joerg; Ward, Richard A

    2009-01-01

    The definition of optimal dialysis therapy remains elusive. Randomized clinical trials have neither supported using urea as a surrogate marker for uremic toxicity nor provided clear cut evidence in favor of larger solutes. Thus, where to focus resources in the development of new membranes, and therapies remains unclear. Three basic questions remain unanswered: (i) what solute(s) should be used as a marker for optimal dialysis; (ii) should dialytic therapies be designed to remove a specific solute; and (iii) how can current therapies be modified to provide better control of uremic toxicity? Identification of a single, well-defined uremic toxin appears to be unlikely as new analytical tools reveal an increasingly complex uremic milieu. As a result, it is probable that membranes and therapies should be designed for the nonspecific removal of a wide variety of solutes retained in uremia. Removal of the widest range of solutes can best be achieved using existing therapies that incorporate convection in conjunction with longer treatment times and more frequent treatments. Membranes capable of removing solutes over an expanded effective molecular size range can already be fabricated; however, their use will require novel approaches to conserve proteins, such as albumin.

  13. Origins Space Telescope: Telescope Design and Instrument Specifications

    NASA Astrophysics Data System (ADS)

    Meixner, Margaret; Carter, Ruth; Leisawitz, David; Dipirro, Mike; Flores, Anel; Staguhn, Johannes; Kellog, James; Roellig, Thomas L.; Melnick, Gary J.; Bradford, Charles; Wright, Edward L.; Zmuidzinas, Jonas; Origins Space Telescope Study Team

    2017-01-01

    The Origins Space Telescope (OST) is the mission concept for the Far-Infrared Surveyor, one of the four science and technology definition studies of NASA Headquarters for the 2020 Astronomy and Astrophysics Decadal survey. The renaming of the mission reflects Origins science goals that will discover and characterize the most distant galaxies, nearby galaxies and the Milky Way, exoplanets, and the outer reaches of our Solar system. This poster will show the preliminary telescope design that will be a large aperture (>8 m in diameter), cryogenically cooled telescope. We will also present the specifications for the spectrographs and imagers over a potential wavelength range of ~10 microns to 1 millimeter. We look forward to community input into this mission definition over the coming year as we work on the concept design for the mission. Origins will enable flagship-quality general observing programs led by the astronomical community in the 2030s. We welcome you to contact the Science and Technology Definition Team (STDT) with your science needs and ideas by emailing us at firsurveyor_info@lists.ipac.caltech.edu.

  14. Program Requirements and Design Specifications for the Quincy School Complex.

    ERIC Educational Resources Information Center

    Quincy School Community Council, MA.

    The Quincy School Complex is a unique community facility. A high level of cooperation has led to the planning of this facility whose operation and ownership is divided among several agencies and groups. The introduction to the report on the school complex provides background material for the program itself, and attempts to define the "why" of the…

  15. 5 CFR 2638.203 - Duties of the designated agency ethics official.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... agency employees concerning all ethics and standards of conduct matters is developed and conducted in...) Initiation and maintenance of ethics education and training programs; and (4) Monitoring administrative... Government Ethics concerning the agency's ethics program is developed and maintained; (2) An effective...

  16. 41 CFR 102-76.55 - What sustainable development principles must Federal agencies apply to the siting, design, and...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... and Construction Sustainable Development § 102-76.55 What sustainable development principles must... Acquisition,” Federal agencies must apply sustainable development principles to the siting, design, and... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false What...

  17. Revisiting software specification and design for large astronomy projects

    NASA Astrophysics Data System (ADS)

    Wiant, Scott; Berukoff, Steven

    2016-07-01

    The separation of science and engineering in the delivery of software systems overlooks the true nature of the problem being solved and the organization that will solve it. Use of a systems engineering approach to managing the requirements flow between these two groups as between a customer and contractor has been used with varying degrees of success by well-known entities such as the U.S. Department of Defense. However, treating science as the customer and engineering as the contractor fosters unfavorable consequences that can be avoided and opportunities that are missed. For example, the "problem" being solved is only partially specified through the requirements generation process since it focuses on detailed specification guiding the parties to a technical solution. Equally important is the portion of the problem that will be solved through the definition of processes and staff interacting through them. This interchange between people and processes is often underrepresented and under appreciated. By concentrating on the full problem and collaborating on a strategy for its solution a science-implementing organization can realize the benefits of driving towards common goals (not just requirements) and a cohesive solution to the entire problem. The initial phase of any project when well executed is often the most difficult yet most critical and thus it is essential to employ a methodology that reinforces collaboration and leverages the full suite of capabilities within the team. This paper describes an integrated approach to specifying the needs induced by a problem and the design of its solution.

  18. Measurement of Spray Drift with a Specifically Designed Lidar System.

    PubMed

    Gregorio, Eduard; Torrent, Xavier; Planas de Martí, Santiago; Solanelles, Francesc; Sanz, Ricardo; Rocadenbosch, Francesc; Masip, Joan; Ribes-Dasi, Manel; Rosell-Polo, Joan R

    2016-04-08

    Field measurements of spray drift are usually carried out by passive collectors and tracers. However, these methods are labour- and time-intensive and only provide point- and time-integrated measurements. Unlike these methods, the light detection and ranging (lidar) technique allows real-time measurements, obtaining information with temporal and spatial resolution. Recently, the authors have developed the first eye-safe lidar system specifically designed for spray drift monitoring. This prototype is based on a 1534 nm erbium-doped glass laser and an 80 mm diameter telescope, has scanning capability, and is easily transportable. This paper presents the results of the first experimental campaign carried out with this instrument. High coefficients of determination (R² > 0.85) were observed by comparing lidar measurements of the spray drift with those obtained by horizontal collectors. Furthermore, the lidar system allowed an assessment of the drift reduction potential (DRP) when comparing low-drift nozzles with standard ones, resulting in a DRP of 57% (preliminary result) for the tested nozzles. The lidar system was also used for monitoring the evolution of the spray flux over the canopy and to generate 2-D images of these plumes. The developed instrument is an advantageous alternative to passive collectors and opens the possibility of new methods for field measurement of spray drift.

  19. Measurement of Spray Drift with a Specifically Designed Lidar System

    PubMed Central

    Gregorio, Eduard; Torrent, Xavier; Planas de Martí, Santiago; Solanelles, Francesc; Sanz, Ricardo; Rocadenbosch, Francesc; Masip, Joan; Ribes-Dasi, Manel; Rosell-Polo, Joan R.

    2016-01-01

    Field measurements of spray drift are usually carried out by passive collectors and tracers. However, these methods are labour- and time-intensive and only provide point- and time-integrated measurements. Unlike these methods, the light detection and ranging (lidar) technique allows real-time measurements, obtaining information with temporal and spatial resolution. Recently, the authors have developed the first eye-safe lidar system specifically designed for spray drift monitoring. This prototype is based on a 1534 nm erbium-doped glass laser and an 80 mm diameter telescope, has scanning capability, and is easily transportable. This paper presents the results of the first experimental campaign carried out with this instrument. High coefficients of determination (R2 > 0.85) were observed by comparing lidar measurements of the spray drift with those obtained by horizontal collectors. Furthermore, the lidar system allowed an assessment of the drift reduction potential (DRP) when comparing low-drift nozzles with standard ones, resulting in a DRP of 57% (preliminary result) for the tested nozzles. The lidar system was also used for monitoring the evolution of the spray flux over the canopy and to generate 2-D images of these plumes. The developed instrument is an advantageous alternative to passive collectors and opens the possibility of new methods for field measurement of spray drift. PMID:27070613

  20. 44 CFR 60.25 - Designation, duties, and responsibilities of State Coordinating Agencies.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... of flood plain management activities with other State, areawide, and local planning and enforcement... EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY INSURANCE AND HAZARD MITIGATION National Flood Insurance Program CRITERIA FOR LAND MANAGEMENT AND USE Additional Considerations in Managing...

  1. 44 CFR 60.25 - Designation, duties, and responsibilities of State Coordinating Agencies.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... of flood plain management activities with other State, areawide, and local planning and enforcement... EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY INSURANCE AND HAZARD MITIGATION National Flood Insurance Program CRITERIA FOR LAND MANAGEMENT AND USE Additional Considerations in Managing...

  2. 5 CFR 5501.102 - Designation of HHS components as separate agencies.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... SUPPLEMENTAL STANDARDS OF ETHICAL CONDUCT FOR EMPLOYEES OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES § 5501... Healthcare Research and Quality; (4) Agency for Toxic Substances and Disease Registry; (5) Centers...

  3. 5 CFR 5501.102 - Designation of HHS components as separate agencies.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... SUPPLEMENTAL STANDARDS OF ETHICAL CONDUCT FOR EMPLOYEES OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES § 5501... Healthcare Research and Quality; (4) Agency for Toxic Substances and Disease Registry; (5) Centers...

  4. Medicare, Medicaid, and Children's Health Insurance Programs: announcement of the extended temporary moratoria on enrollment of ambulance suppliers and home health agencies in designated geographic locations. Extension of temporary moratoria.

    PubMed

    2014-08-01

    This document announces the extension of temporary moratoria on the enrollment of new ambulance suppliers and home health agencies (HHAs) in specific locations within designated metropolitan areas in Florida, Illinois, Michigan, Texas, Pennsylvania, and New Jersey to prevent and combat fraud, waste, and abuse.

  5. Design of a System That Understands Informal Specifications.

    DTIC Science & Technology

    1983-04-01

    Identify. by block numib., formal specifications, English specifications, modules, natural language processing, abstract data types, logic-programming...Horn clauses 20. ABSTRACT (Continue an reverse aide if necessary and Identify by block numiber) This paper describes a system for understanding English ...University of Delaware Newark, DE 19711 28 April 1983 ABSTRACT This paper describes a system for understanding English definitions of software modules

  6. A requirements specification for a software design support system

    NASA Technical Reports Server (NTRS)

    Noonan, Robert E.

    1988-01-01

    Most existing software design systems (SDSS) support the use of only a single design methodology. A good SDSS should support a wide variety of design methods and languages including structured design, object-oriented design, and finite state machines. It might seem that a multiparadigm SDSS would be expensive in both time and money to construct. However, it is proposed that instead an extensible SDSS that directly implements only minimal database and graphical facilities be constructed. In particular, it should not directly implement tools to faciliate language definition and analysis. It is believed that such a system could be rapidly developed and put into limited production use, with the experience gained used to refine and evolve the systems over time.

  7. Government Agencies

    DTIC Science & Technology

    1988-05-01

    manufacturers. The Navy has a major in- house design capability for ships; the government does not possess such a capability for aircraft or other weapon systems...the Coast Guard, government agencies acquire a wide variety of ships, ranging from sophisticated submarines and nuclear aircraft carriers to much...the initial phase a review was made of written material relating to government procedures in U.S. Government agencies for acquiring vessels, aircraft

  8. 34 CFR 395.3 - Application for designation as State licensing agency; content.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... agency to the vendors, including their selection, duties, supervision, transfer, promotion, financial... vocational potential of each blind vendor is achieved; (3) The policies and standards to be employed in the selection of suitable locations for vending facilities; (4) The methods to be used to ensure the...

  9. 5 CFR 5501.102 - Designation of HHS components as separate agencies.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... remainder of HHS: (1) Administration on Aging; (2) Administration for Children and Families: (3) Agency for... Disease Control and Prevention; (6) Centers for Medicare and Medicaid Services; (7) Food and Drug... identify an employee's “agency” for purposes of: (1) Determining when a person is a prohibited...

  10. 46 CFR 162.050-25 - Cargo monitor: Design specification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... manually mixing a known quantity of oil and a known quantity of water to form a mixture and manually... cause formation of static electricity. (e) A monitor must be designed to operate in each plane...

  11. 46 CFR 162.050-25 - Cargo monitor: Design specification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... manually mixing a known quantity of oil and a known quantity of water to form a mixture and manually... cause formation of static electricity. (e) A monitor must be designed to operate in each plane...

  12. NASA software specification and evaluation system design, part 1

    NASA Technical Reports Server (NTRS)

    1976-01-01

    The research to develop methods for reducing the effort expended in software and verification is reported. The development of a formal software requirements methodology, a formal specifications language, a programming language, a language preprocessor, and code analysis tools are discussed.

  13. Electromagnetic sunscreen model: design of experiments on particle specifications.

    PubMed

    Lécureux, Marie; Deumié, Carole; Enoch, Stefan; Sergent, Michelle

    2015-10-01

    We report a numerical study on sunscreen design and optimization. Thanks to the combined use of electromagnetic modeling and design of experiments, we are able to screen the most relevant parameters of mineral filters and to optimize sunscreens. Several electromagnetic modeling methods are used depending on the type of particles, density of particles, etc. Both the sun protection factor (SPF) and the UVB/UVA ratio are considered. We show that the design of experiments' model should include interactions between materials and other parameters. We conclude that the material of the particles is a key parameter for the SPF and the UVB/UVA ratio. Among the materials considered, none is optimal for both. The SPF is also highly dependent on the size of the particles.

  14. Formal design specification of a Processor Interface Unit

    NASA Technical Reports Server (NTRS)

    Fura, David A.; Windley, Phillip J.; Cohen, Gerald C.

    1992-01-01

    This report describes work to formally specify the requirements and design of a processor interface unit (PIU), a single-chip subsystem providing memory-interface bus-interface, and additional support services for a commercial microprocessor within a fault-tolerant computer system. This system, the Fault-Tolerant Embedded Processor (FTEP), is targeted towards applications in avionics and space requiring extremely high levels of mission reliability, extended maintenance-free operation, or both. The need for high-quality design assurance in such applications is an undisputed fact, given the disastrous consequences that even a single design flaw can produce. Thus, the further development and application of formal methods to fault-tolerant systems is of critical importance as these systems see increasing use in modern society.

  15. Automated Tactical Symbology System (TACSYM): System Design Specifications

    DTIC Science & Technology

    1984-03-01

    138. Personal Demand 39. Microphones 89. CBR 139. Repair Parts 40. Target Designator 90. Chemical 140. Wire 41. Visual Station 91. Combined Arms...allmi for modification of the database. Insertion and removal of data from the database is controlled by DATABsE . C. 2.1.4 Software Suppot t. The

  16. Requirement Specifications for a Design and Verification Unit.

    ERIC Educational Resources Information Center

    Pelton, Warren G.; And Others

    A research and development activity to introduce new and improved education and training technology into Bureau of Medicine and Surgery training is recommended. The activity, called a design and verification unit, would be administered by the Education and Training Sciences Department. Initial research and development are centered on the…

  17. As-built design specification for segment map (Sgmap) program

    NASA Technical Reports Server (NTRS)

    Tompkins, M. A. (Principal Investigator)

    1981-01-01

    The segment map program (SGMAP), which is part of the CLASFYT package, is described in detail. This program is designed to output symbolic maps or numerical dumps from LANDSAT cluster/classification files or aircraft ground truth/processed ground truth files which are in 'universal' format.

  18. Specifications for a COM Catalog Designed for Government Documents.

    ERIC Educational Resources Information Center

    Copeland, Nora S.; And Others

    Prepared in MARC format in accordance with the Ohio College Library Center (OCLC) standards, these specifications were developed at Colorado State University to catalog a group of government publications not listed in the Monthly Catalog of United States Publications. The resulting microfiche catalog produced through the OCLC Cataloging Subsystem…

  19. NASIS data base management system: IBM 360 TSS implementation. Volume 4: Program design specifications

    NASA Technical Reports Server (NTRS)

    1973-01-01

    The design specifications for the programs and modules within the NASA Aerospace Safety Information System (NASIS) are presented. The purpose of the design specifications is to standardize the preparation of the specifications and to guide the program design. Each major functional module within the system is a separate entity for documentation purposes. The design specifications contain a description of, and specifications for, all detail processing which occurs in the module. Sub-models, reference tables, and data sets which are common to several modules are documented separately.

  20. A review of design issues specific to hypersonic flight vehicles

    NASA Astrophysics Data System (ADS)

    Sziroczak, D.; Smith, H.

    2016-07-01

    This paper provides an overview of the current technical issues and challenges associated with the design of hypersonic vehicles. Two distinct classes of vehicles are reviewed; Hypersonic Transports and Space Launchers, their common features and differences are examined. After a brief historical overview, the paper takes a multi-disciplinary approach to these vehicles, discusses various design aspects, and technical challenges. Operational issues are explored, including mission profiles, current and predicted markets, in addition to environmental effects and human factors. Technological issues are also reviewed, focusing on the three major challenge areas associated with these vehicles: aerothermodynamics, propulsion, and structures. In addition, matters of reliability and maintainability are also presented. The paper also reviews the certification and flight testing of these vehicles from a global perspective. Finally the current stakeholders in the field of hypersonic flight are presented, summarizing the active programs and promising concepts.

  1. Septa design for a prostate specific PET camera

    SciTech Connect

    Qi, Jinyi; Huber, Jennifer S.; Huesman, Ronald H.; Moses, William W.; Derenzo, Stephen E.; Budinger, Thomas F.

    2003-11-15

    The recent development of new prostate tracers has motivated us to build a low cost PET camera optimized to image the prostate. Coincidence imaging of positron emitters is achieved using a pair of external curved detector banks. The bottom bank is fixed below the patient bed, and the top bank moves upward for patient access and downward for maximum sensitivity. In this paper, we study the design of septa for the prostate camera using Monte Carlo simulations. The system performance is measured by the detectability of a prostate lesion. We have studied 17 septa configurations. The results show that the design of septa has a large impact on the lesion detection at a given activity concentration. Significant differences are also observed between the lesion detectability and the conventional noise equivalent count (NEC) performance, indicating that the NEC is not appropriate for the detection task.

  2. Rational design of a triple helix-specific intercalating ligand.

    PubMed

    Escudé, C; Nguyen, C H; Kukreti, S; Janin, Y; Sun, J S; Bisagni, E; Garestier, T; Hélène, C

    1998-03-31

    DNA triple helices offer new perspectives toward oligonucleotide-directed gene regulation. However, the poor stability of some of these structures might limit their use under physiological conditions. Specific ligands can intercalate into DNA triple helices and stabilize them. Molecular modeling and thermal denaturation experiments suggest that benzo[f]pyrido[3, 4-b]quinoxaline derivatives intercalate into triple helices by stacking preferentially with the Hoogsteen-paired bases. Based on this model, it was predicted that a benzo[f]quino[3,4-b]quinoxaline derivative, which possesses an additional aromatic ring, could engage additional stacking interactions with the pyrimidine strand of the Watson-Crick double helix upon binding of this pentacyclic ligand to a triplex structure. This compound was synthesized. Thermal denaturation experiments and inhibition of restriction enzyme cleavage show that this new compound can indeed stabilize triple helices with great efficiency and specificity and/or induce triple helix formation under physiological conditions.

  3. The extended PP1 toolkit: designed to create specificity

    PubMed Central

    Bollen, Mathieu; Peti, Wolfgang; Ragusa, Michael J.; Beullens, Monique

    2011-01-01

    Protein Ser/Thr phosphatase-1 (PP1) catalyzes the majority of eukaryotic protein dephosphorylation reactions in a highly regulated and selective manner. Recent studies have identified an unusually diversified PP1 interactome with the properties of a regulatory toolkit. PP1-interacting proteins (PIPs) function as targeting subunits, substrates and/or inhibitors. As targeting subunits, PIPs contribute to substrate selection by bringing PP1 into the vicinity of specific substrates and by modulating substrate specificity via additional substrate docking sites or blocking substrate-binding channels. Many of the nearly 200 established mammalian PIPs are predicted to be intrinsically disordered, a property that facilitates their binding to a large surface area of PP1 via multiple docking motifs. These novel insights offer perspectives for the therapeutic targeting of PP1 by interfering with the binding of PIPs or substrates. PMID:20399103

  4. 14 CFR 91.705 - Operations within airspace designated as Minimum Navigation Performance Specification Airspace.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Minimum Navigation Performance Specification Airspace. 91.705 Section 91.705 Aeronautics and Space FEDERAL... Operations within airspace designated as Minimum Navigation Performance Specification Airspace. (a) Except as... airspace designated as Minimum Navigation Performance Specifications airspace unless— (1) The aircraft...

  5. Novel designed enediynes: molecular design, chemical synthesis, mode of cycloaromatization and guanine-specific DNA cleavage.

    PubMed

    Toshima, K; Ohta, K; Kano, T; Nakamura, T; Nakata, M; Kinoshita, M; Matsumura, S

    1996-01-01

    The molecular design and chemical synthesis of novel enediyne molecules related to the neocarzinostatin chromophore (1), and their chemical and DNA cleaving properties are described. The 10-membered enediyne triols 16-18 were effectively synthesized from xylitol (10) in a short step, and found to be quite stable when handled at room temperature. The representative and acylated enediyne 16 was cycloaromatized by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) in cyclohexa-1,4-diene-benzene to give the benzenoid product 21 through a radical pathway. On the other hand, the enediyne 16 was cycloaromatized by diethylamine in dimethyl sulfoxide-Tris-HCl, pH 8.5 buffer to afford another benzenoid product 22 as a diethylamine adduct through a polar pathway. Furthermore, the enediynes 16-18 were found to exhibit guanine-specific DNA cleavage under weakly basic conditions with no additive.

  6. 41 CFR 102-76.10 - What basic design and construction policy governs Federal agencies?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 41 Public Contracts and Property Management 3 2014-01-01 2014-01-01 false What basic design and... Management Federal Property Management Regulations System (Continued) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION General Provisions § 102-76.10 What basic design and...

  7. 41 CFR 102-76.10 - What basic design and construction policy governs Federal agencies?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 41 Public Contracts and Property Management 3 2013-07-01 2013-07-01 false What basic design and... Management Federal Property Management Regulations System (Continued) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION General Provisions § 102-76.10 What basic design and...

  8. 41 CFR 102-76.10 - What basic design and construction policy governs Federal agencies?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false What basic design and... Management Federal Property Management Regulations System (Continued) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION General Provisions § 102-76.10 What basic design and...

  9. 41 CFR 102-76.10 - What basic design and construction policy governs Federal agencies?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 41 Public Contracts and Property Management 3 2011-01-01 2011-01-01 false What basic design and... Management Federal Property Management Regulations System (Continued) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION General Provisions § 102-76.10 What basic design and...

  10. 41 CFR 102-76.10 - What basic design and construction policy governs Federal agencies?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 41 Public Contracts and Property Management 3 2012-01-01 2012-01-01 false What basic design and... Management Federal Property Management Regulations System (Continued) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 76-DESIGN AND CONSTRUCTION General Provisions § 102-76.10 What basic design and...

  11. Restraint system design and evaluation for military specific applications

    NASA Astrophysics Data System (ADS)

    Karwaczynski, Sebastian

    This research focuses on designing an optimal restraint system for usage in a military vehicle applications. The designed restraint system must accommodate a wide range of DHM's and ATD's with and without PPE such as: helmet, boots, and body armor. The evaluation of the restraint systems were conducted in a simulated vehicle environment, which was utilized to downselect the ideal restraint system for this program. In December of 2011 the OCP TECD program was formulated to increase occupant protection. To do this, 3D computer models were created to accommodate the entire Soldier population in the Army. These models included the entire PPE, which were later utilized for space claim activities and for designing new seats and restraints, which would accommodate them. Additionally, guidelines to increase protection levels while providing optimal comfort to the Soldier were created. The current and emerging threats were evaluated and focused on at the time of the program inception. Throughout this program various activities were conducted for restraint downselection including Soldier evaluations of various restraint system configurations. The Soldiers were given an opportunity to evaluate each system in a representative seat, which allowed them to position themselves in a manner consistent with the mission requirements. Systems ranged from fully automated to manual adjustment type systems. An evaluation of each particular system was conducted and analyzed against the other systems. It was discovered that the restraint systems, which utilize retractors allowed for automatic webbing stowage and allowed for easier access and repeatability when donning and doffing the restraint. It was also found that when an aid was introduced to help the Soldier don the restraint, it was more likely that such system would be utilized. Restraints were evaluated in drop tower experiments in addition to actual blast tests. An evaluation with this amount of detail had not been attempted

  12. Advanced Free Flight Planner and Dispatcher's Workstation: Preliminary Design Specification

    NASA Technical Reports Server (NTRS)

    Wilson, J.; Wright, C.; Couluris, G. J.

    1997-01-01

    The National Aeronautics and Space Administration (NASA) has implemented the Advanced Air Transportation Technology (AATT) program to investigate future improvements to the national and international air traffic management systems. This research, as part of the AATT program, developed preliminary design requirements for an advanced Airline Operations Control (AOC) dispatcher's workstation, with emphasis on flight planning. This design will support the implementation of an experimental workstation in NASA laboratories that would emulate AOC dispatch operations. The work developed an airline flight plan data base and specified requirements for: a computer tool for generation and evaluation of free flight, user preferred trajectories (UPT); the kernel of an advanced flight planning system to be incorporated into the UPT-generation tool; and an AOC workstation to house the UPT-generation tool and to provide a real-time testing environment. A prototype for the advanced flight plan optimization kernel was developed and demonstrated. The flight planner uses dynamic programming to search a four-dimensional wind and temperature grid to identify the optimal route, altitude and speed for successive segments of a flight. An iterative process is employed in which a series of trajectories are successively refined until the LTPT is identified. The flight planner is designed to function in the current operational environment as well as in free flight. The free flight environment would enable greater flexibility in UPT selection based on alleviation of current procedural constraints. The prototype also takes advantage of advanced computer processing capabilities to implement more powerful optimization routines than would be possible with older computer systems.

  13. Home Care Nursing via Computer Networks: Justification and Design Specifications

    PubMed Central

    Brennan, Patricia Flatley

    1988-01-01

    High-tech home care includes the use of information technologies, such as computer networks, to provide direct care to patients in the home. This paper presents the justification and design of a project using a free, public access computer network to deliver home care nursing. The intervention attempts to reduce isolation and improve problem solving among home care patients and their informal caregivers. Three modules comprise the intervention: a decision module, a communications module, and an information data base. This paper describes the experimental evaluation of the project, and discusses issues in the delivery of nursing care via computers.

  14. Cooperativity and specificity of association of a designed transmembrane peptide.

    PubMed Central

    Gratkowski, Holly; Dai, Qing-Hong; Wand, A Joshua; DeGrado, William F; Lear, James D

    2002-01-01

    Thermodynamics studies aimed at quantitatively characterizing free energy effects of amino acid substitutions are not restricted to two state systems, but do require knowing the number of states involved in the equilibrium under consideration. Using analytical ultracentrifugation and NMR methods, we show here that a membrane-soluble peptide, MS1, designed by modifying the sequence of the water-soluble coiled-coil GCN4-P1, exhibits a reversible monomer-dimer-trimer association in detergent micelles with a greater degree of cooperativity in C14-betaine than in dodecyl phosphocholine detergents. PMID:12202385

  15. 23 CFR 636.107 - May contracting agencies use geographic preference in Federal-aid design-build or public-private...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 23 Highways 1 2011-04-01 2011-04-01 false May contracting agencies use geographic preference in Federal-aid design-build or public-private partnership projects? 636.107 Section 636.107 Highways FEDERAL... or public-private partnership projects? No. Contracting agencies must not use geographic...

  16. 23 CFR 636.107 - May contracting agencies use geographic preference in Federal-aid design-build or public-private...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false May contracting agencies use geographic preference in Federal-aid design-build or public-private partnership projects? 636.107 Section 636.107 Highways FEDERAL... or public-private partnership projects? No. Contracting agencies must not use geographic...

  17. Heat pipe design handbook, part 2. [digital computer code specifications

    NASA Technical Reports Server (NTRS)

    Skrabek, E. A.

    1972-01-01

    The utilization of a digital computer code for heat pipe analysis and design (HPAD) is described which calculates the steady state hydrodynamic heat transport capability of a heat pipe with a particular wick configuration, the working fluid being a function of wick cross-sectional area. Heat load, orientation, operating temperature, and heat pipe geometry are specified. Both one 'g' and zero 'g' environments are considered, and, at the user's option, the code will also perform a weight analysis and will calculate heat pipe temperature drops. The central porous slab, circumferential porous wick, arterial wick, annular wick, and axial rectangular grooves are the wick configurations which HPAD has the capability of analyzing. For Vol. 1, see N74-22569.

  18. An ultrasound-guided fluorescence tomography system: design and specification

    NASA Astrophysics Data System (ADS)

    D'Souza, Alisha V.; Flynn, Brendan P.; Kanick, Stephen C.; Torosean, Sason; Davis, Scott C.; Maytin, Edward V.; Hasan, Tayyaba; Pogue, Brian W.

    2013-03-01

    An ultrasound-guided fluorescence molecular tomography system is under development for in vivo quantification of Protoporphyrin IX (PpIX) during Aminolevulinic Acid - Photodynamic Therapy (ALA-PDT) of Basal Cell Carcinoma. The system is designed to combine fiber-based spectral sampling of PPIX fluorescence emission with co-registered ultrasound images to quantify local fluorophore concentration. A single white light source is used to provide an estimate of the bulk optical properties of tissue. Optical data is obtained by sequential illumination of a 633nm laser source at 4 linear locations with parallel detection at 5 locations interspersed between the sources. Tissue regions from segmented ultrasound images, optical boundary data, white light-informed optical properties and diffusion theory are used to estimate the fluorophore concentration in these regions. Our system and methods allow interrogation of both superficial and deep tissue locations up to PpIX concentrations of 0.025ug/ml.

  19. 24 CFR 3282.361 - Design Approval Primary Inspection Agency (DAPIA).

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... deviations from accepted engineering practice standards for design calculations or any deviations from..., and test records of the structural, electrical and mechanical systems of each such manufactured...

  20. Overall plant design specification Modular High Temperature Gas-cooled Reactor. Revision 9

    SciTech Connect

    1990-05-01

    Revision 9 of the ``Overall Plant Design Specification Modular High Temperature Gas-Cooled Reactor,`` DOE-HTGR-86004 (OPDS) has been completed and is hereby distributed for use by the HTGR Program team members. This document, Revision 9 of the ``Overall Plant Design Specification`` (OPDS) reflects those changes in the MHTGR design requirements and configuration resulting form approved Design Change Proposals DCP BNI-003 and DCP BNI-004, involving the Nuclear Island Cooling and Spent Fuel Cooling Systems respectively.

  1. Design Thinking and the Deanly Conversation: Reflections on Conversation, Community, and Agency

    ERIC Educational Resources Information Center

    Campbell, Katy

    2015-01-01

    The article "Conversation as Inquiry: A Conversation with Instructional Designers" (Campbell, Schwier & Kenny 2006) appeared in the "Journal of Learning Design" Volume 1, Issue 3 in 2006. Nine years on, Professor Katy Campbell, Dean of the Faculty of Extension, University of Alberta, reflects upon the arguments articulated…

  2. Design and implementation of an inter-agency, multi-mission space flight operations network interface

    NASA Technical Reports Server (NTRS)

    Byrne, R.; Scharf, M.; Doan, D.; Liu, J.; Willems, A.

    2004-01-01

    An advanced network interface was designed and implemented by a team from the Jet Propulsion Lab with support from the European Space Operations Center. This poster shows the requirements for the interface, the design, the topology, the testing and lessons learned from the whole implementation.

  3. Specification and preliminary design of an array processor

    NASA Technical Reports Server (NTRS)

    Slotnick, D. L.; Graham, M. L.

    1975-01-01

    The design of a computer suited to the class of problems typified by the general circulation of the atmosphere was investigated. A fundamental goal was that the resulting machine should have roughly 100 times the computing capability of an IBM 360/95 computer. A second requirement was that the machine should be programmable in a higher level language similar to FORTRAN. Moreover, the new machine would have to be compatible with the IBM 360/95 since the IBM machine would continue to be used for pre- and post-processing. A third constraint was that the cost of the new machine was to be significantly less than that of other extant machines of similar computing capability, such as the ILLIAC IV and CDC STAR. A final constraint was that it should be feasible to fabricate a complete system and put it in operation by early 1978. Although these objectives were generally met, considerable work remains to be done on the routing system.

  4. Psychosocial Risks Generated By Assets Specific Design Software

    NASA Astrophysics Data System (ADS)

    Remus, Furtună; Angela, Domnariu; Petru, Lazăr

    2015-07-01

    The human activity concerning an occupation is resultant from the interaction between the psycho-biological, socio-cultural and organizational-occupational factors. Tehnological development, automation and computerization that are to be found in all the branches of activity, the level of speed in which things develop, as well as reaching their complexity, require less and less physical aptitudes and more cognitive qualifications. The person included in the work process is bound in most of the cases to come in line with the organizational-occupational situations that are specific to the demands of the job. The role of the programmer is essencial in the process of execution of ordered softwares, thus the truly brilliant ideas can only come from well-rested minds, concentrated on their tasks. The actual requirements of the jobs, besides the high number of benefits and opportunities, also create a series of psycho-social risks, which can increase the level of stress during work activity, especially for those who work under pressure.

  5. Liquid Engine Design: Effect of Chamber Dimensions on Specific Impulse

    NASA Technical Reports Server (NTRS)

    Hoggard, Lindsay; Leahy, Joe

    2009-01-01

    Which assumption of combustion chemistry - frozen or equilibrium - should be used in the prediction of liquid rocket engine performance calculations? Can a correlation be developed for this? A literature search using the LaSSe tool, an online repository of old rocket data and reports, was completed. Test results of NTO/Aerozine-50 and Lox/LH2 subscale and full-scale injector and combustion chamber test results were found and studied for this task. NASA code, Chemical Equilibrium with Applications (CEA) was used to predict engine performance using both chemistry assumptions, defined here. Frozen- composition remains frozen during expansion through the nozzle. Equilibrium- instantaneous chemical equilibrium during nozzle expansion. Chamber parameters were varied to understand what dimensions drive chamber C* and Isp. Contraction Ratio is the ratio of the nozzle throat area to the area of the chamber. L is the length of the chamber. Characteristic chamber length, L*, is the length that the chamber would be if it were a straight tube and had no converging nozzle. Goal: Develop a qualitative and quantitative correlation for performance parameters - Specific Impulse (Isp) and Characteristic Velocity (C*) - as a function of one or more chamber dimensions - Contraction Ratio (CR), Chamber Length (L ) and/or Characteristic Chamber Length (L*). Determine if chamber dimensions can be correlated to frozen or equilibrium chemistry.

  6. Earth Observatory Satellite system definition study. Report no. 5: System design and specifications. Part 1: Observatory system element specifications

    NASA Technical Reports Server (NTRS)

    1974-01-01

    The performance, design, and quality assurance requirements for the Earth Observatory Satellite (EOS) Observatory and Ground System program elements required to perform the Land Resources Management (LRM) A-type mission are presented. The requirements for the Observatory element with the exception of the instruments specifications are contained in the first part.

  7. Software design specification. Part 2: Orbital Flight Test (OFT) detailed design specification. Volume 3: Applications. Book 2: System management

    NASA Technical Reports Server (NTRS)

    1979-01-01

    The functions performed by the systems management (SM) application software are described along with the design employed to accomplish these functions. The operational sequences (OPS) control segments and the cyclic processes they control are defined. The SM specialist function control (SPEC) segments and the display controlled 'on-demand' processes that are invoked by either an OPS or SPEC control segment as a direct result of an item entry to a display are included. Each processing element in the SM application is described including an input/output table and a structured control flow diagram. The flow through the module and other information pertinent to that process and its interfaces to other processes are included.

  8. 24 CFR 3282.361 - Design Approval Primary Inspection Agency (DAPIA).

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... innovations which are not yet accepted practices. Acceptances by the Secretary shall be published in the form... the manufacturing process. Within 5 days after approving a design change, the DAPIA shall forward...

  9. 49 CFR 107.402 - Application for designation as an approval or certification agency.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS AND OIL TRANSPORTATION HAZARDOUS MATERIALS PROGRAM PROCEDURES Designation of Approval and... Associate Administrator for Hazardous Materials Safety (PHH-32), Department of Transportation, East...

  10. 49 CFR 107.402 - Application for designation as an approval or certification agency.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS AND OIL TRANSPORTATION HAZARDOUS MATERIALS PROGRAM PROCEDURES Designation of Approval and... Associate Administrator for Hazardous Materials Safety (PHH-32), Department of Transportation, East...

  11. 44 CFR 60.25 - Designation, duties, and responsibilities of State Coordinating Agencies.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., Mudslide (i.e., Mudflow)-Prone and Flood-Related Erosion-Prone Areas § 60.25 Designation, duties, and... this part and in conformance with other Federal and State environmental and water pollution...

  12. SP-Designer: a user-friendly program for designing species-specific primer pairs from DNA sequence alignments.

    PubMed

    Villard, Pierre; Malausa, Thibaut

    2013-07-01

    SP-Designer is an open-source program providing a user-friendly tool for the design of specific PCR primer pairs from a DNA sequence alignment containing sequences from various taxa. SP-Designer selects PCR primer pairs for the amplification of DNA from a target species on the basis of several criteria: (i) primer specificity, as assessed by interspecific sequence polymorphism in the annealing regions, (ii) the biochemical characteristics of the primers and (iii) the intended PCR conditions. SP-Designer generates tables, detailing the primer pair and PCR characteristics, and a FASTA file locating the primer sequences in the original sequence alignment. SP-Designer is Windows-compatible and freely available from http://www2.sophia.inra.fr/urih/sophia_mart/sp_designer/info_sp_designer.php.

  13. GSP: A web-based platform for designing genome-specific primers in polyploids

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The sequences among subgenomes in a polyploid species have high similarity. This makes difficult to design genome-specific primers for sequence analysis. We present a web-based platform named GSP for designing genome-specific primers to distinguish subgenome sequences in the polyploid genome backgr...

  14. 29 CFR 1960.6 - Designation of agency safety and health officials.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Order 12196, and this part; (2) An organization, including provision for the designation of safety and health officials at appropriate levels, with adequate budgets and staffs to implement the occupational safety and health program at all operational levels; (3) A set of procedures that ensures...

  15. 29 CFR 1960.6 - Designation of agency safety and health officials.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Order 12196, and this part; (2) An organization, including provision for the designation of safety and health officials at appropriate levels, with adequate budgets and staffs to implement the occupational safety and health program at all operational levels; (3) A set of procedures that ensures...

  16. 29 CFR 1960.6 - Designation of agency safety and health officials.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Order 12196, and this part; (2) An organization, including provision for the designation of safety and health officials at appropriate levels, with adequate budgets and staffs to implement the occupational safety and health program at all operational levels; (3) A set of procedures that ensures...

  17. Preliminary design of the Carrisa Plains solar central receiver power plant. Volume II. Plant specifications

    SciTech Connect

    Price, R. E.

    1983-12-31

    The specifications and design criteria for all plant systems and subsystems used in developing the preliminary design of Carrisa Plains 30-MWe Solar Plant are contained in this volume. The specifications have been organized according to plant systems and levels. The levels are arranged in tiers. Starting at the top tier and proceeding down, the specification levels are the plant, system, subsystem, components, and fabrication. A tab number, listed in the index, has been assigned each document to facilitate document location.

  18. Designing high specificity anti-cancer nanocarriers by exploiting non-equilibrium effects

    NASA Astrophysics Data System (ADS)

    Tsekouras, Konstantinos; Goncharenko, Igor; Colvin, Michael; Huang, Kerwyn; Gopinathan, Ajay

    2012-11-01

    Although targeting of cancer cells using drug-delivering nanocarriers holds promise for improving therapeutic agent specificity, the strategy of maximizing ligand affinity for receptors overexpressed on cancer cells is suboptimal. To determine design principles that maximize nanocarrier specificity for cancer cells, we studied a generalized kinetics-based theoretical model of nanocarriers with one or more ligands that specifically bind these overexpressed receptors. We show that kinetics inherent to the system play an important role in determining specificity and can in fact be exploited to attain orders of magnitude improvement in specificity. In contrast to the current trend of therapeutic design, we show that these specificity increases can generally be achieved by a combination of low rates of endocytosis and nanocarriers with multiple low-affinity ligands. These results are broadly robust across endocytosis mechanisms and drug-delivery protocols, suggesting the need for a paradigm shift in receptor- targeted drug-delivery design.

  19. Collaborative design and use of an agency feedback form for student clinical practicum experience in community/public health nursing.

    PubMed

    Reilly, Janet Resop; Collier, Jill; Edelstein, Janice; Vandenhouten, Chris; Hovarter, Rebecca; Hansen, Judith M; Stewart, Stephanie; Turner, Mary Jo

    2012-01-01

    Evaluation of students in community and public health (C/PH) nursing clinical practica is a challenge, especially when preceptors are expected to evaluate students from different academic nursing programs. The need for a standardized student evaluation tool was identified during federally funded collaborative meetings held between C/PH academic and practice partners in Northeastern Wisconsin. This article focuses on the development and appraisal of the standardized Agency Feedback Form (AFF) for Student Practicum Experience in Community/Public Health Nursing, which was designed to meet the identified need. Four baccalaureate nursing programs implemented the AFF for 3 purposes: (1) to provide a consistent and easy evaluation form for preceptors to complete; (2) to communicate useful information about students' individual professional behaviors observed during practicum; and (3) to increase students' and preceptors' understanding of the population-based nursing interventions, using the Public Health Intervention Wheel. Future uses and implications of the AFF are also discussed.

  20. Design Specifications for the Advanced Instructional Design Advisor (AIDA). Volume 1

    DTIC Science & Technology

    1992-01-01

    task order contract ( Task 6). 1.2.2 Instructional Design Guidelines: Functional Requirements The second thrust attempts to identify optimal methods...Training technicians to interpret written instructions is (or should be) more a matter of designing the instructions than training the students. The task of...troubleshooting action against an optimal trouble- shooting model so that it can suggest more fruitful approaches at appropriate times. The optimal model used by

  1. 36 CFR 907.5 - Specific responsibilities of designated Corporation official.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Corporation's planning and decision-making processes to ensure that environmental factors are properly... DEVELOPMENT CORPORATION ENVIRONMENTAL QUALITY § 907.5 Specific responsibilities of designated Corporation... pertaining to environmental protection and enhancement. (b) Establish and maintain working relationships...

  2. 32 CFR 319.13 - Specific exemptions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... PROGRAM DEFENSE INTELLIGENCE AGENCY PRIVACY PROGRAM § 319.13 Specific exemptions. (a) All systems of records maintained by the Director Intelligence Agency shall be exempt from the requirements of 5 U.S.C... has been properly classified. (b) The Director, Defense Intelligence Agency, designated the systems...

  3. 32 CFR 319.13 - Specific exemptions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... PROGRAM DEFENSE INTELLIGENCE AGENCY PRIVACY PROGRAM § 319.13 Specific exemptions. (a) All systems of records maintained by the Director Intelligence Agency shall be exempt from the requirements of 5 U.S.C... has been properly classified. (b) The Director, Defense Intelligence Agency, designated the systems...

  4. Practical Findings from Applying the PSD Model for Evaluating Software Design Specifications

    NASA Astrophysics Data System (ADS)

    Räisänen, Teppo; Lehto, Tuomas; Oinas-Kukkonen, Harri

    This paper presents practical findings from applying the PSD model to evaluating the support for persuasive features in software design specifications for a mobile Internet device. On the one hand, our experiences suggest that the PSD model fits relatively well for evaluating design specifications. On the other hand, the model would benefit from more specific heuristics for evaluating each technique to avoid unnecessary subjectivity. Better distinction between the design principles in the social support category would also make the model easier to use. Practitioners who have no theoretical background can apply the PSD model to increase the persuasiveness of the systems they design. The greatest benefit of the PSD model for researchers designing new systems may be achieved when it is applied together with a sound theory, such as the Elaboration Likelihood Model. Using the ELM together with the PSD model, one may increase the chances for attitude change.

  5. Automatic generation of conceptual database design tools from data model specifications

    SciTech Connect

    Hong, Shuguang.

    1989-01-01

    The problems faced in the design and implementation of database software systems based on object-oriented data models are similar to that of other software design, i.e., difficult, complex, yet redundant effort. Automatic generation of database software system has been proposed as a solution to the problems. In order to generate database software system for a variety of object-oriented data models, two critical issues: data model specification and software generation, must be addressed. SeaWeed is a software system that automatically generates conceptual database design tools from data model specifications. A meta model has been defined for the specification of a class of object-oriented data models. This meta model provides a set of primitive modeling constructs that can be used to express the semantics, or unique characteristics, of specific data models. Software reusability has been adopted for the software generation. The technique of design reuse is utilized to derive the requirement specification of the software to be generated from data model specifications. The mechanism of code reuse is used to produce the necessary reusable software components. This dissertation presents the research results of SeaWeed including the meta model, data model specification, a formal representation of design reuse and code reuse, and the software generation paradigm.

  6. HAL/SM system functional design specification. [systems analysis and design analysis of central processing units

    NASA Technical Reports Server (NTRS)

    Ross, C.; Williams, G. P. W., Jr.

    1975-01-01

    The functional design of a preprocessor, and subsystems is described. A structure chart and a data flow diagram are included for each subsystem. Also a group of intermodule interface definitions (one definition per module) is included immediately following the structure chart and data flow for a particular subsystem. Each of these intermodule interface definitions consists of the identification of the module, the function the module is to perform, the identification and definition of parameter interfaces to the module, and any design notes associated with the module. Also described are compilers and computer libraries.

  7. Earth Observatory Satellite system definition study. Report 5: System design and specifications. Volume 5: Specification for EROS operations control center

    NASA Technical Reports Server (NTRS)

    1974-01-01

    The functional, performance, and design requirements for the Operations Control Center (OCC) of the Earth Observatory Satellite (EOS) system are presented. The OCC controls the operations of the EOS satellite to acquire mission data consisting of: (1) thematic mapper data, (2) multispectral scanner data on EOS-A, or High Resolution Pointable Imager data on EOS-B, and (3) data collection system (DCS) data. The various inputs to the OCC are identified. The functional requirements of the OCC are defined. The specific systems and subsystems of the OCC are described and block diagrams are provided.

  8. Better Educational Website Interface Design: The Implications from Gender-Specific Preferences in Graduate Students

    ERIC Educational Resources Information Center

    Hsu, Yu-chang

    2006-01-01

    This study investigated graduate students gender-specific preferences for certain website interface design features, intending to generate useful information for instructors in choosing and for website designers in creating educational websites. The features investigated in this study included colour value, major navigation buttons placement, and…

  9. Single Event Testing on Complex Devices: Test Like You Fly versus Test-Specific Design Structures

    NASA Technical Reports Server (NTRS)

    Berg, Melanie; LaBel, Kenneth A.

    2014-01-01

    We present a framework for evaluating complex digital systems targeted for harsh radiation environments such as space. Focus is limited to analyzing the single event upset (SEU) susceptibility of designs implemented inside Field Programmable Gate Array (FPGA) devices. Tradeoffs are provided between application-specific versus test-specific test structures.

  10. 36 CFR 907.5 - Specific responsibilities of designated Corporation official.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false Specific responsibilities of... DEVELOPMENT CORPORATION ENVIRONMENTAL QUALITY § 907.5 Specific responsibilities of designated Corporation... parties information and advice on the Corporation's policies for protecting and enhancing the quality...

  11. A domain-specific design architecture for composite material design and aircraft part redesign

    NASA Technical Reports Server (NTRS)

    Punch, W. F., III; Keller, K. J.; Bond, W.; Sticklen, J.

    1992-01-01

    Advanced composites have been targeted as a 'leapfrog' technology that would provide a unique global competitive position for U.S. industry. Composites are unique in the requirements for an integrated approach to designing, manufacturing, and marketing of products developed utilizing the new materials of construction. Numerous studies extending across the entire economic spectrum of the United States from aerospace to military to durable goods have identified composites as a 'key' technology. In general there have been two approaches to composite construction: build models of a given composite materials, then determine characteristics of the material via numerical simulation and empirical testing; and experience-directed construction of fabrication plans for building composites with given properties. The first route sets a goal to capture basic understanding of a device (the composite) by use of a rigorous mathematical model; the second attempts to capture the expertise about the process of fabricating a composite (to date) at a surface level typically expressed in a rule based system. From an AI perspective, these two research lines are attacking distinctly different problems, and both tracks have current limitations. The mathematical modeling approach has yielded a wealth of data but a large number of simplifying assumptions are needed to make numerical simulation tractable. Likewise, although surface level expertise about how to build a particular composite may yield important results, recent trends in the KBS area are towards augmenting surface level problem solving with deeper level knowledge. Many of the relative advantages of composites, e.g., the strength:weight ratio, is most prominent when the entire component is designed as a unitary piece. The bottleneck in undertaking such unitary design lies in the difficulty of the re-design task. Designing the fabrication protocols for a complex-shaped, thick section composite are currently very difficult. It is in

  12. Laser communication experiment. Volume 1: Design study report: Spacecraft transceiver. Part 3: LCE design specifications

    NASA Technical Reports Server (NTRS)

    1970-01-01

    The requirements for the design, fabrication, performance, and testing of a 10.6 micron optical heterodyne receiver subsystem for use in a laser communication system are presented. The receiver subsystem, as a part of the laser communication experiment operates in the ATS 6 satellite and in a transportable ground station establishing two-way laser communications between the spacecraft and the transportable ground station. The conditions under which environmental tests are conducted are reported.

  13. Designing specific protein-protein interactions using computation, experimental library screening, or integrated methods.

    PubMed

    Chen, T Scott; Keating, Amy E

    2012-07-01

    Given the importance of protein-protein interactions for nearly all biological processes, the design of protein affinity reagents for use in research, diagnosis or therapy is an important endeavor. Engineered proteins would ideally have high specificities for their intended targets, but achieving interaction specificity by design can be challenging. There are two major approaches to protein design or redesign. Most commonly, proteins and peptides are engineered using experimental library screening and/or in vitro evolution. An alternative approach involves using protein structure and computational modeling to rationally choose sequences predicted to have desirable properties. Computational design has successfully produced novel proteins with enhanced stability, desired interactions and enzymatic function. Here we review the strengths and limitations of experimental library screening and computational structure-based design, giving examples where these methods have been applied to designing protein interaction specificity. We highlight recent studies that demonstrate strategies for combining computational modeling with library screening. The computational methods provide focused libraries predicted to be enriched in sequences with the properties of interest. Such integrated approaches represent a promising way to increase the efficiency of protein design and to engineer complex functionality such as interaction specificity.

  14. Development of an integrated CAD-FEA system for patient-specific design of spinal cages.

    PubMed

    Zhang, Mingzheng; Pu, Fang; Xu, Liqiang; Zhang, Linlin; Liang, Hang; Li, Deyu; Wang, Yu; Fan, Yubo

    2017-03-01

    Spinal cages are used to create a suitable mechanical environment for interbody fusion in cases of degenerative spinal instability. Due to individual variations in bone structures and pathological conditions, patient-specific cages can provide optimal biomechanical conditions for fusion, strengthening patient recovery. Finite element analysis (FEA) is a valuable tool in the biomechanical evaluation of patient-specific cage designs, but the time- and labor-intensive process of modeling limits its clinical application. In an effort to facilitate the design and analysis of patient-specific spinal cages, an integrated CAD-FEA system (CASCaDeS, comprehensive analytical spinal cage design system) was developed. This system produces a biomechanical-based patient-specific design of spinal cages and is capable of rapid implementation of finite element modeling. By comparison with commercial software, this system was validated and proven to be both accurate and efficient. CASCaDeS can be used to design patient-specific cages with a superior biomechanical performance to commercial spinal cages.

  15. From ergonomics to design specifications: contributions to the design of a processing machine in a tire company.

    PubMed

    Moraes, A S P; Arezes, P M; Vasconcelos, R

    2012-01-01

    The development of ergonomics' recommendations, guidelines and standards are attempts to promote the integration of ergonomics into industrial contexts. Such developments result from several sources and professionals and represent the effort that has been done to develop healthier and safer work environments. However, the availability of large amount of data and documents regarding ergonomics does not guarantee their applicability. The main goal of this paper is to use a specific case to demonstrate how ergonomics criteria were developed in order to contribute to the design of workplaces. Based on the obtained results from research undertaken in a tire company, it was observed that the ergonomics criteria should be presented as design specifications in order to be used by engineers and designers. In conclusion, it is observed that the multiple constraint environment impeded the appliance of the ergonomics criteria. It was also observed that the knowledge on technical design and the acquaintance with ergonomic standards, the level of integration in the design team, and the ability to communicate with workers and other technical staff have paramount importance in integrating ergonomics criteria into the design process.

  16. Specification and Design of Electrical Flight System Architectures with SysML

    NASA Technical Reports Server (NTRS)

    McKelvin, Mark L., Jr.; Jimenez, Alejandro

    2012-01-01

    Modern space flight systems are required to perform more complex functions than previous generations to support space missions. This demand is driving the trend to deploy more electronics to realize system functionality. The traditional approach for the specification, design, and deployment of electrical system architectures in space flight systems includes the use of informal definitions and descriptions that are often embedded within loosely coupled but highly interdependent design documents. Traditional methods become inefficient to cope with increasing system complexity, evolving requirements, and the ability to meet project budget and time constraints. Thus, there is a need for more rigorous methods to capture the relevant information about the electrical system architecture as the design evolves. In this work, we propose a model-centric approach to support the specification and design of electrical flight system architectures using the System Modeling Language (SysML). In our approach, we develop a domain specific language for specifying electrical system architectures, and we propose a design flow for the specification and design of electrical interfaces. Our approach is applied to a practical flight system.

  17. Custom-Designed Molecular Scissors for Site-Specific Manipulation of the Plant and Mammalian Genomes

    NASA Astrophysics Data System (ADS)

    Kandavelou, Karthikeyan; Chandrasegaran, Srinivasan

    Zinc finger nucleases (ZFNs) are custom-designed molecular scissors, engineered to cut at specific DNA sequences. ZFNs combine the zinc finger proteins (ZFPs) with the nonspecific cleavage domain of the FokI restriction enzyme. The DNA-binding specificity of ZFNs can be easily altered experimentally. This easy manipulation of the ZFN recognition specificity enables one to deliver a targeted double-strand break (DSB) to a genome. The targeted DSB stimulates local gene targeting by several orders of magnitude at that specific cut site via homologous recombination (HR). Thus, ZFNs have become an important experimental tool to make site-specific and permanent alterations to genomes of not only plants and mammals but also of many other organisms. Engineering of custom ZFNs involves many steps. The first step is to identify a ZFN site at or near the chosen chromosomal target within the genome to which ZFNs will bind and cut. The second step is to design and/or select various ZFP combinations that will bind to the chosen target site with high specificity and affinity. The DNA coding sequence for the designed ZFPs are then assembled by polymerase chain reaction (PCR) using oligonucleotides. The third step is to fuse the ZFP constructs to the FokI cleavage domain. The ZFNs are then expressed as proteins by using the rabbit reticulocyte in vitro transcription/translation system and the protein products assayed for their DNA cleavage specificity.

  18. Computationally designed high specificity inhibitors delineate the roles of BCL2 family proteins in cancer

    PubMed Central

    Berger, Stephanie; Procko, Erik; Margineantu, Daciana; Lee, Erinna F; Shen, Betty W; Zelter, Alex; Silva, Daniel-Adriano; Chawla, Kusum; Herold, Marco J; Garnier, Jean-Marc; Johnson, Richard; MacCoss, Michael J; Lessene, Guillaume; Davis, Trisha N; Stayton, Patrick S; Stoddard, Barry L; Fairlie, W Douglas; Hockenbery, David M; Baker, David

    2016-01-01

    Many cancers overexpress one or more of the six human pro-survival BCL2 family proteins to evade apoptosis. To determine which BCL2 protein or proteins block apoptosis in different cancers, we computationally designed three-helix bundle protein inhibitors specific for each BCL2 pro-survival protein. Following in vitro optimization, each inhibitor binds its target with high picomolar to low nanomolar affinity and at least 300-fold specificity. Expression of the designed inhibitors in human cancer cell lines revealed unique dependencies on BCL2 proteins for survival which could not be inferred from other BCL2 profiling methods. Our results show that designed inhibitors can be generated for each member of a closely-knit protein family to probe the importance of specific protein-protein interactions in complex biological processes. DOI: http://dx.doi.org/10.7554/eLife.20352.001 PMID:27805565

  19. Computationally designed high specificity inhibitors delineate the roles of BCL2 family proteins in cancer.

    PubMed

    Berger, Stephanie; Procko, Erik; Margineantu, Daciana; Lee, Erinna F; Shen, Betty W; Zelter, Alex; Silva, Daniel-Adriano; Chawla, Kusum; Herold, Marco J; Garnier, Jean-Marc; Johnson, Richard; MacCoss, Michael J; Lessene, Guillaume; Davis, Trisha N; Stayton, Patrick S; Stoddard, Barry L; Fairlie, W Douglas; Hockenbery, David M; Baker, David

    2016-11-02

    Many cancers overexpress one or more of the six human pro-survival BCL2 family proteins to evade apoptosis. To determine which BCL2 protein or proteins block apoptosis in different cancers, we computationally designed three-helix bundle protein inhibitors specific for each BCL2 pro-survival protein. Following in vitro optimization, each inhibitor binds its target with high picomolar to low nanomolar affinity and at least 300-fold specificity. Expression of the designed inhibitors in human cancer cell lines revealed unique dependencies on BCL2 proteins for survival which could not be inferred from other BCL2 profiling methods. Our results show that designed inhibitors can be generated for each member of a closely-knit protein family to probe the importance of specific protein-protein interactions in complex biological processes.

  20. Structural, kinetic, and thermodynamic studies of specificity designed HIV-1 protease

    SciTech Connect

    Alvizo, Oscar; Mittal, Seema; Mayo, Stephen L.; Schiffer, Celia A.

    2012-10-23

    HIV-1 protease recognizes and cleaves more than 12 different substrates leading to viral maturation. While these substrates share no conserved motif, they are specifically selected for and cleaved by protease during viral life cycle. Drug resistant mutations evolve within the protease that compromise inhibitor binding but allow the continued recognition of all these substrates. While the substrate envelope defines a general shape for substrate recognition, successfully predicting the determinants of substrate binding specificity would provide additional insights into the mechanism of altered molecular recognition in resistant proteases. We designed a variant of HIV protease with altered specificity using positive computational design methods and validated the design using X-ray crystallography and enzyme biochemistry. The engineered variant, Pr3 (A28S/D30F/G48R), was designed to preferentially bind to one out of three of HIV protease's natural substrates; RT-RH over p2-NC and CA-p2. In kinetic assays, RT-RH binding specificity for Pr3 increased threefold compared to the wild-type (WT), which was further confirmed by isothermal titration calorimetry. Crystal structures of WT protease and the designed variant in complex with RT-RH, CA-p2, and p2-NC were determined. Structural analysis of the designed complexes revealed that one of the engineered substitutions (G48R) potentially stabilized heterogeneous flap conformations, thereby facilitating alternate modes of substrate binding. Our results demonstrate that while substrate specificity could be engineered in HIV protease, the structural pliability of protease restricted the propagation of interactions as predicted. These results offer new insights into the plasticity and structural determinants of substrate binding specificity of the HIV-1 protease.

  1. Using Space Weather Variability in Evaluating the Radiation Environment Design Specifications for NASA's Constellation Program

    NASA Technical Reports Server (NTRS)

    Coffey, Victoria N.; Blackwell, William C.; Minow, Joseph I.; Bruce, Margaret B.; Howard, James W.

    2007-01-01

    NASA's Constellation program, initiated to fulfill the Vision for Space Exploration, will create a new generation of vehicles for servicing low Earth orbit, the Moon, and beyond. Space radiation specifications for space system hardware are necessarily conservative to assure system robustness for a wide range of space environments. Spectral models of solar particle events and trapped radiation belt environments are used to develop the design requirements for estimating total ionizing radiation dose, displacement damage, and single event effects for Constellation hardware. We first describe the rationale using the spectra chosen to establish the total dose and single event design environmental specifications for Constellation systems. We then compare variability of the space environment to the spectral design models to evaluate their applicability as conservative design environments and potential vulnerabilities to extreme space weather events

  2. Computational design of a red fluorophore ligase for site-specific protein labeling in living cells

    SciTech Connect

    Liu, Daniel S.; Nivon, Lucas G.; Richter, Florian; Goldman, Peter J.; Deerinck, Thomas J.; Yao, Jennifer Z.; Richardson, Douglas; Phipps, William S.; Ye, Anne Z.; Ellisman, Mark H.; Drennan, Catherine L.; Baker, David; Ting, Alice Y.

    2014-10-13

    In this study, chemical fluorophores offer tremendous size and photophysical advantages over fluorescent proteins but are much more challenging to target to specific cellular proteins. Here, we used Rosetta-based computation to design a fluorophore ligase that accepts the red dye resorufin, starting from Escherichia coli lipoic acid ligase. X-ray crystallography showed that the design closely matched the experimental structure. Resorufin ligase catalyzed the site-specific and covalent attachment of resorufin to various cellular proteins genetically fused to a 13-aa recognition peptide in multiple mammalian cell lines and in primary cultured neurons. We used resorufin ligase to perform superresolution imaging of the intermediate filament protein vimentin by stimulated emission depletion and electron microscopies. This work illustrates the power of Rosetta for major redesign of enzyme specificity and introduces a tool for minimally invasive, highly specific imaging of cellular proteins by both conventional and superresolution microscopies.

  3. Computational design of a red fluorophore ligase for site-specific protein labeling in living cells

    DOE PAGES

    Liu, Daniel S.; Nivon, Lucas G.; Richter, Florian; ...

    2014-10-13

    In this study, chemical fluorophores offer tremendous size and photophysical advantages over fluorescent proteins but are much more challenging to target to specific cellular proteins. Here, we used Rosetta-based computation to design a fluorophore ligase that accepts the red dye resorufin, starting from Escherichia coli lipoic acid ligase. X-ray crystallography showed that the design closely matched the experimental structure. Resorufin ligase catalyzed the site-specific and covalent attachment of resorufin to various cellular proteins genetically fused to a 13-aa recognition peptide in multiple mammalian cell lines and in primary cultured neurons. We used resorufin ligase to perform superresolution imaging of themore » intermediate filament protein vimentin by stimulated emission depletion and electron microscopies. This work illustrates the power of Rosetta for major redesign of enzyme specificity and introduces a tool for minimally invasive, highly specific imaging of cellular proteins by both conventional and superresolution microscopies.« less

  4. Designing patient-specific 3D printed craniofacial implants using a novel topology optimization method.

    PubMed

    Sutradhar, Alok; Park, Jaejong; Carrau, Diana; Nguyen, Tam H; Miller, Michael J; Paulino, Glaucio H

    2016-07-01

    Large craniofacial defects require efficient bone replacements which should not only provide good aesthetics but also possess stable structural function. The proposed work uses a novel multiresolution topology optimization method to achieve the task. Using a compliance minimization objective, patient-specific bone replacement shapes can be designed for different clinical cases that ensure revival of efficient load transfer mechanisms in the mid-face. In this work, four clinical cases are introduced and their respective patient-specific designs are obtained using the proposed method. The optimized designs are then virtually inserted into the defect to visually inspect the viability of the design . Further, once the design is verified by the reconstructive surgeon, prototypes are fabricated using a 3D printer for validation. The robustness of the designs are mechanically tested by subjecting them to a physiological loading condition which mimics the masticatory activity. The full-field strain result through 3D image correlation and the finite element analysis implies that the solution can survive the maximum mastication of 120 lb. Also, the designs have the potential to restore the buttress system and provide the structural integrity. Using the topology optimization framework in designing the bone replacement shapes would deliver surgeons new alternatives for rather complicated mid-face reconstruction.

  5. Method for Predicting the Energy Characteristics of Li-Ion Cells Designed for High Specific Energy

    NASA Technical Reports Server (NTRS)

    Bennett, William, R.

    2012-01-01

    Novel electrode materials with increased specific capacity and voltage performance are critical to the NASA goals for developing Li-ion batteries with increased specific energy and energy density. Although performance metrics of the individual electrodes are critically important, a fundamental understanding of the interactions of electrodes in a full cell is essential to achieving the desired performance, and for establishing meaningful goals for electrode performance in the first place. This paper presents design considerations for matching positive and negative electrodes in a viable design. Methods for predicting cell-level performance, based on laboratory data for individual electrodes, are presented and discussed.

  6. Mod-5A wind turbine generator program design report. Volume 4: Drawings and specifications, book 3

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The design, development and analysis of the 7.3 MW MOD-5A wind turbine generator is documented. This volume contains the drawings and specifications developed for the final design. This volume is divided into 5 books of which this is the third, containing drawings 47A380074 through 47A380126. A full breakdown parts listing is provided as well as a where used list.

  7. 48 CFR 52.246-19 - Warranty of Systems and Equipment under Performance Specifications or Design Criteria.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Equipment under Performance Specifications or Design Criteria. 52.246-19 Section 52.246-19 Federal... under Performance Specifications or Design Criteria. As prescribed in 46.710(c)(1), the contracting... Specifications or Design Criteria (MAY 2001) (a) Definitions. As used in this clause— Acceptance means the act...

  8. Design of Optimal Treatments for Neuromusculoskeletal Disorders using Patient-Specific Multibody Dynamic Models

    PubMed Central

    Fregly, Benjamin J.

    2011-01-01

    Disorders of the human neuromusculoskeletal system such as osteoarthritis, stroke, cerebral palsy, and paraplegia significantly affect mobility and result in a decreased quality of life. Surgical and rehabilitation treatment planning for these disorders is based primarily on static anatomic measurements and dynamic functional measurements filtered through clinical experience. While this subjective treatment planning approach works well in many cases, it does not predict accurate functional outcome in many others. This paper presents a vision for how patient-specific multibody dynamic models can serve as the foundation for an objective treatment planning approach that identifies optimal treatments and treatment parameters on an individual patient basis. First, a computational paradigm is presented for constructing patient-specific multibody dynamic models. This paradigm involves a combination of patient-specific skeletal models, muscle-tendon models, neural control models, and articular contact models, with the complexity of the complete model being dictated by the requirements of the clinical problem being addressed. Next, three clinical applications are presented to illustrate how such models could be used in the treatment design process. One application involves the design of patient-specific gait modification strategies for knee osteoarthritis rehabilitation, a second involves the selection of optimal patient-specific surgical parameters for a particular knee osteoarthritis surgery, and the third involves the design of patient-specific muscle stimulation patterns for stroke rehabilitation. The paper concludes by discussing important challenges that need to be overcome to turn this vision into reality. PMID:21785529

  9. The Environmental Protection Agency: Legislation, Programs and Organization.

    ERIC Educational Resources Information Center

    Environmental Protection Agency, Washington, DC. Library Systems Branch.

    This document is designed to introduce the Environmental Protection Agency (EPA) and its programs. It is organized into four categories which discuss the legislation authorizing EPA activities, the history and present organization, the pollution control programs operated by the Agency, and budgetary appropriations. Specific program…

  10. Mod-5A Wind Turbine Generator Program Design Report. Volume 4: Drawings and Specifications, Book 1

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The design, development and analysis of the 7.3 MW MOD-5A wind turbine generator is documented. Volume 4 contains the drawings and specifications that were developed in preparation for building the MOD-5A wind turbine generator. This is the first of five books of volume four. It contains structural design criteria, generator step-up transformer specs, specs for design, fabrication and testing of the system, specs for the ground control enclosure, systems specs, slip ring specs, and control system specs.

  11. Just-in-time Design and Additive Manufacture of Patient-specific Medical Implants

    NASA Astrophysics Data System (ADS)

    Shidid, Darpan; Leary, Martin; Choong, Peter; Brandt, Milan

    Recent advances in medical imaging and manufacturing science have enabled the design and production of complex, patient-specific orthopaedic implants. Additive Manufacture (AM) generates three-dimensional structures layer by layer, and is not subject to the constraints associated with traditional manufacturing methods. AM provides significant opportunities for the design of novel geometries and complex lattice structures with enhanced functional performance. However, the design and manufacture of patient-specific AM implant structures requires unique expertise in handling various optimization platforms. Furthermore, the design process for complex structures is computationally intensive. The primary aim of this research is to enable the just-in-time customisation of AM prosthesis; whereby AM implant design and manufacture be completed within the time constraints of a single surgical procedure, while minimising prosthesis mass and optimising the lattice structure to match the stiffness of the surrounding bone tissue. In this research, a design approach using raw CT scan data is applied to the AM manufacture of femoral prosthesis. Using the proposed just-in-time concept, the mass of the prosthesis was rapidly designed and manufactured while satisfying the associated structural requirements. Compressive testing of lattice structures manufactured using proposed method shows that the load carrying capacity of the resected composite bone can be recovered by up to 85% and the compressive stiffness of the AM prosthesis is statistically indistinguishable from the stiffness of the initial bone.

  12. Towards the formal specification of the requirements and design of a processor interface unit: HOL listings

    NASA Technical Reports Server (NTRS)

    Fura, David A.; Windley, Phillip J.; Cohen, Gerald C.

    1993-01-01

    This technical report contains the HOL listings of the specification of the design and major portions of the requirements for a commercially developed processor interface unit (or PIU). The PIU is an interface chip performing memory interface, bus interface, and additional support services for a commercial microprocessor within a fault-tolerant computer system. This system, the Fault-Tolerant Embedded Processor (FTEP), is targeted towards applications in avionics and space requiring extremely high levels of mission reliability, extended maintenance-free operation, or both. This report contains the actual HOL listings of the PIU specification as it currently exists. Section two of this report contains general-purpose HOL theories that support the PIU specification. These theories include definitions for the hardware components used in the PIU, our implementation of bit words, and our implementation of temporal logic. Section three contains the HOL listings for the PIU design specification. Aside from the PIU internal bus (I-Bus), this specification is complete. Section four contains the HOL listings for a major portion of the PIU requirements specification. Specifically, it contains most of the definition for the PIU behavior associated with memory accesses initiated by the local processor.

  13. Assessing cost-effectiveness of specific LID practice designs in response to large storm events

    NASA Astrophysics Data System (ADS)

    Chui, Ting Fong May; Liu, Xin; Zhan, Wenting

    2016-02-01

    Low impact development (LID) practices have become more important in urban stormwater management worldwide. However, most research on design optimization focuses on relatively large scale, and there is very limited information or guideline regarding individual LID practice designs (i.e., optimal depth, width and length). The objective of this study is to identify the optimal design by assessing the hydrological performance and the cost-effectiveness of different designs of LID practices at a household or business scale, and to analyze the sensitivity of the hydrological performance and the cost of the optimal design to different model and design parameters. First, EPA SWMM, automatically controlled by MATLAB, is used to obtain the peak runoff of different designs of three specific LID practices (i.e., green roof, bioretention and porous pavement) under different design storms (i.e., 2 yr and 50 yr design storms of Hong Kong, China and Seattle, U.S.). Then, life cycle cost is estimated for the different designs, and the optimal design, defined as the design with the lowest cost and at least 20% peak runoff reduction, is identified. Finally, sensitivity of the optimal design to the different design parameters is examined. The optimal design of green roof tends to be larger in area but thinner, while the optimal designs of bioretention and porous pavement tend to be smaller in area. To handle larger storms, however, it is more effective to increase the green roof depth, and to increase the area of the bioretention and porous pavement. Porous pavement is the most cost-effective for peak flow reduction, followed by bioretention and then green roof. The cost-effectiveness, measured as the peak runoff reduction/thousand Dollars of LID practices in Hong Kong (e.g., 0.02 L/103 US s, 0.15 L/103 US s and 0.93 L/103 US s for green roof, bioretention and porous pavement for 2 yr storm) is lower than that in Seattle (e.g., 0.03 L/103 US s, 0.29 L/103 US s and 1.58 L/103 US s for

  14. Spaceborne computer executive routine functional design specification. Volume 2: Computer executive design for space station/base

    NASA Technical Reports Server (NTRS)

    Kennedy, J. R.; Fitzpatrick, W. S.

    1971-01-01

    The computer executive functional system design concepts derived from study of the Space Station/Base are presented. Information Management System hardware configuration as directly influencing the executive design is reviewed. The hardware configuration and generic executive design requirements are considered in detail in a previous report (System Configuration and Executive Requirements Specifications for Reusable Shuttle and Space Station/Base, 9/25/70). This report defines basic system primitives and delineates processes and process control. Supervisor states are considered for describing basic multiprogramming and multiprocessing systems. A high-level computer executive including control of scheduling, allocation of resources, system interactions, and real-time supervisory functions is defined. The description is oriented to provide a baseline for a functional simulation of the computer executive system.

  15. Design and Development of a Model to Simulate 0-G Treadmill Running Using the European Space Agency's Subject Loading System

    NASA Technical Reports Server (NTRS)

    Caldwell, E. C.; Cowley, M. S.; Scott-Pandorf, M. M.

    2010-01-01

    Develop a model that simulates a human running in 0 G using the European Space Agency s (ESA) Subject Loading System (SLS). The model provides ground reaction forces (GRF) based on speed and pull-down forces (PDF). DESIGN The theoretical basis for the Running Model was based on a simple spring-mass model. The dynamic properties of the spring-mass model express theoretical vertical GRF (GRFv) and shear GRF in the posterior-anterior direction (GRFsh) during running gait. ADAMs VIEW software was used to build the model, which has a pelvis, thigh segment, shank segment, and a spring foot (see Figure 1).the model s movement simulates the joint kinematics of a human running at Earth gravity with the aim of generating GRF data. DEVELOPMENT & VERIFICATION ESA provided parabolic flight data of subjects running while using the SLS, for further characterization of the model s GRF. Peak GRF data were fit to a linear regression line dependent on PDF and speed. Interpolation and extrapolation of the regression equation provided a theoretical data matrix, which is used to drive the model s motion equations. Verification of the model was conducted by running the model at 4 different speeds, with each speed accounting for 3 different PDF. The model s GRF data fell within a 1-standard-deviation boundary derived from the empirical ESA data. CONCLUSION The Running Model aids in conducting various simulations (potential scenarios include a fatigued runner or a powerful runner generating high loads at a fast cadence) to determine limitations for the T2 vibration isolation system (VIS) aboard the International Space Station. This model can predict how running with the ESA SLS affects the T2 VIS and may be used for other exercise analyses in the future.

  16. Mod-5A wind turbine generator program design report. Volume 4: Drawings and specifications, book 5

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The design, development and analysis of the 7.3 MW MOD-5A wind turbine generator is documented. There are four volumes. This volume contains the drawings and specifications that were developed in preparation for building the MOD-5A wind turbine generator. Detail drawings of several assemblies and subassemblies are given. This is the fifth book of volume 4.

  17. GSP: a web-based platform for designing genome-specific primers in polyploids

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The primary goal of this research was to develop a web-based platform named GSP for designing genome-specific primers to distinguish subgenome sequences in the polyploid genome background. GSP uses BLAST to extract homeologous sequences of the subgenomes in the existing databases, performed a multip...

  18. Investigating an Open Methodology for Designing Domain-Specific Language Collections

    ERIC Educational Resources Information Center

    Fitzgerald, Alannah; Wu, Shaoqun; Barge, Martin

    2014-01-01

    With this research and design paper, we are proposing that Open Educational Resources (OERs) and Open Access (OA) publications give increasing access to high quality online educational and research content for the development of powerful domain-specific language collections that can be further enhanced linguistically with the Flexible Language…

  19. Designing Peace and Conflict Exercises: Level of Analysis, Scenario, and Role Specification

    ERIC Educational Resources Information Center

    Bartels, Elizabeth; McCown, Margaret; Wilkie, Timothy

    2013-01-01

    Attentiveness to and transparency about the methodological implications of the level of analysis selected for peace and conflict exercises constitute essential elements of good game design. The article explores the impact of level of analysis choices in the context of two key portions of exercises, scenario construction and role specification. It…

  20. Systems design study of the Pioneer Venus spacecraft. Volume 3. Specifications

    NASA Technical Reports Server (NTRS)

    1973-01-01

    Pioneer Venus spacecraft performance requirements are presented. The specifications include: (1) Design criteria and performance requirements for the Pioneer Venus spacecraft systems and subsystems for a 1978 multiprobe mission and a 1978 orbiter mission, spacecraft system interface, and scientific instrument integration.

  1. System design and specifications. Earth Observatory Satellite system definition study (EOS)

    NASA Technical Reports Server (NTRS)

    1974-01-01

    A design summary of the Earth Observatory Satellite (EOS) is presented. The systems considered in the summary are: (1) the spacecraft structure, (2) electrical power modules, (3) communications and data handling module, (4) attitude determination module, (5) actuation module, and (6) solar array and drive module. The documents which provide the specifications for the systems and the equipment are identified.

  2. Design, synthesis, and characterization of nucleosomes containing site-specific DNA damage.

    PubMed

    Taylor, John-Stephen

    2015-12-01

    How DNA damaged is formed, recognized, and repaired in chromatin is an area of intense study. To better understand the structure activity relationships of damaged chromatin, mono and dinucleosomes containing site-specific damage have been prepared and studied. This review will focus on the design, synthesis, and characterization of model systems of damaged chromatin for structural, physical, and enzymatic studies.

  3. Functional design specification for Stowage List And Hardware Tracking System (SLAHTS). [space shuttles

    NASA Technical Reports Server (NTRS)

    Keltner, D. J.

    1975-01-01

    This functional design specification defines the total systems approach to meeting the requirements stated in the Detailed Requirements Document for Stowage List and Hardware Tracking System for the space shuttle program. The stowage list and hardware tracking system is identified at the system and subsystem level with each subsystem defined as a function of the total system.

  4. 24 CFR 3280.904 - Specific requirements for designing the transportation system.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... the transportation system. 3280.904 Section 3280.904 Housing and Urban Development Regulations... SAFETY STANDARDS Transportation § 3280.904 Specific requirements for designing the transportation system. (a) General. The entire system (frame, drawbar and coupling mechanism, running gear assembly,...

  5. A bibliography on formal methods for system specification, design and validation

    NASA Technical Reports Server (NTRS)

    Meyer, J. F.; Furchtgott, D. G.; Movaghar, A.

    1982-01-01

    Literature on the specification, design, verification, testing, and evaluation of avionics systems was surveyed, providing 655 citations. Journal papers, conference papers, and technical reports are included. Manual and computer-based methods were employed. Keywords used in the online search are listed.

  6. Rational design of antibodies targeting specific epitopes within intrinsically disordered proteins.

    PubMed

    Sormanni, Pietro; Aprile, Francesco A; Vendruscolo, Michele

    2015-08-11

    Antibodies are powerful tools in life sciences research, as well as in diagnostic and therapeutic applications, because of their ability to bind given molecules with high affinity and specificity. Using current methods, however, it is laborious and sometimes difficult to generate antibodies to target specific epitopes within a protein, in particular if these epitopes are not effective antigens. Here we present a method to rationally design antibodies to enable them to bind virtually any chosen disordered epitope in a protein. The procedure consists in the sequence-based design of one or more complementary peptides targeting a selected disordered epitope and the subsequent grafting of such peptides on an antibody scaffold. We illustrate the method by designing six single-domain antibodies to bind different epitopes within three disease-related intrinsically disordered proteins and peptides (α-synuclein, Aβ42, and IAPP). Our results show that all these designed antibodies bind their targets with good affinity and specificity. As an example of an application, we show that one of these antibodies inhibits the aggregation of α-synuclein at substoichiometric concentrations and that binding occurs at the selected epitope. Taken together, these results indicate that the design strategy that we propose makes it possible to obtain antibodies targeting given epitopes in disordered proteins or protein regions.

  7. 41 CFR 102-118.275 - What must my agency consider when designing and implementing a prepayment audit program?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... services, your agency should ensure that each TSP bill or employee travel voucher contains enough... under $100.00 shall be retained on-site by the contractor and made available for on-site audits....

  8. 41 CFR 102-118.275 - What must my agency consider when designing and implementing a prepayment audit program?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... services, your agency should ensure that each TSP bill or employee travel voucher contains enough... under $100.00 shall be retained on-site by the contractor and made available for on-site audits....

  9. 41 CFR 102-118.275 - What must my agency consider when designing and implementing a prepayment audit program?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... services, your agency should ensure that each TSP bill or employee travel voucher contains enough... under $100.00 shall be retained on-site by the contractor and made available for on-site audits....

  10. Testing Requirements to Manage Data Exchange Specifications in Enterprise Integration - A Schema Design Quality Focus.

    SciTech Connect

    Kulvatunyou, Boonserm; Ivezic, Nenad; Buhwan, Jeong

    2004-07-01

    In this paper, we describe the requirements to test W3C XML Schema usage when defining message schemas for data exchange in any large and evolving enterprise integration project. We then decompose the XML Schema testing into four (4) aspects including the message schema conformance to the XML Schema specification grammar, the message schema conformance to the XML Schema specification semantics, the message schema conformance to design quality testing, and canonical semantics testing of the message schema. We describe these four testing aspects in some detail and point to other related efforts. We further focus to provide some technical details for the message schema design quality testing. As a future work, we describe the requirements for canonical semantics testing and potential solution approaches. Finally, we describe an implementation architecture for the message schema design quality testing.

  11. Computational design of intermolecular stability and specificity in protein self-assembly.

    PubMed

    Nanda, Vikas; Zahid, Sohail; Xu, Fei; Levine, Daniel

    2011-01-01

    The ability to engineer novel proteins using the principles of molecular structure and energetics is a stringent test of our basic understanding of how proteins fold and maintain structure. The design of protein self-assembly has the potential to impact many fields of biology from molecular recognition to cell signaling to biomaterials. Most progress in computational design of protein self-assembly has focused on α-helical systems, exploring ways to concurrently optimize the stability and specificity of a target state. Applying these methods to collagen self-assembly is very challenging, due to fundamental differences in folding and structure of α- versus triple-helices. Here, we explore various computational methods for designing stable and specific oligomeric systems, with a focus on α-helix and collagen self-assembly.

  12. Enriching Peptide Libraries for Binding Affinity and Specificity Through Computationally Directed Library Design.

    PubMed

    Foight, Glenna Wink; Chen, T Scott; Richman, Daniel; Keating, Amy E

    2017-01-01

    Peptide reagents with high affinity or specificity for their target protein interaction partner are of utility for many important applications. Optimization of peptide binding by screening large libraries is a proven and powerful approach. Libraries designed to be enriched in peptide sequences that are predicted to have desired affinity or specificity characteristics are more likely to yield success than random mutagenesis. We present a library optimization method in which the choice of amino acids to encode at each peptide position can be guided by available experimental data or structure-based predictions. We discuss how to use analysis of predicted library performance to inform rounds of library design. Finally, we include protocols for more complex library design procedures that consider the chemical diversity of the amino acids at each peptide position and optimize a library score based on a user-specified input model.

  13. Design, Specification, and Synthesis of Aircraft Electric Power Systems Control Logic

    NASA Astrophysics Data System (ADS)

    Xu, Huan

    Cyber-physical systems integrate computation, networking, and physical processes. Substantial research challenges exist in the design and verification of such large-scale, distributed sensing, actuation, and control systems. Rapidly improving technology and recent advances in control theory, networked systems, and computer science give us the opportunity to drastically improve our approach to integrated flow of information and cooperative behavior. Current systems rely on text-based specifications and manual design. Using new technology advances, we can create easier, more efficient, and cheaper ways of developing these control systems. This thesis will focus on design considerations for system topologies, ways to formally and automatically specify requirements, and methods to synthesize reactive control protocols, all within the context of an aircraft electric power system as a representative application area. This thesis consists of three complementary parts: synthesis, specification, and design. The first section focuses on the synthesis of central and distributed reactive controllers for an aircraft elec- tric power system. This approach incorporates methodologies from computer science and control. The resulting controllers are correct by construction with respect to system requirements, which are formulated using the specification language of linear temporal logic (LTL). The second section addresses how to formally specify requirements and introduces a domain-specific language for electric power systems. A software tool automatically converts high-level requirements into LTL and synthesizes a controller. The final sections focus on design space exploration. A design methodology is proposed that uses mixed-integer linear programming to obtain candidate topologies, which are then used to synthesize controllers. The discrete-time control logic is then verified in real-time by two methods: hardware and simulation. Finally, the problem of partial observability and

  14. Design and component specifications for high average power laser optical systems

    SciTech Connect

    O'Neil, R.W.; Sawicki, R.H.; Johnson, S.A.; Sweatt, W.C.

    1987-01-01

    Laser imaging and transport systems are considered in the regime where laser-induced damage and/or thermal distortion have significant design implications. System design and component specifications are discussed and quantified in terms of the net system transport efficiency and phase budget. Optical substrate materials, figure, surface roughness, coatings, and sizing are considered in the context of visible and near-ir optical systems that have been developed at Lawrence Livermore National Laboratory for laser isotope separation applications. In specific examples of general applicability, details of the bulk and/or surface absorption, peak and/or average power damage threshold, coating characteristics and function, substrate properties, or environmental factors will be shown to drive the component size, placement, and shape in high-power systems. To avoid overstressing commercial fabrication capabilities or component design specifications, procedures will be discussed for compensating for aberration buildup, using a few carefully placed adjustable mirrors. By coupling an aggressive measurements program on substrates and coatings to the design effort, an effective technique has been established to project high-power system performance realistically and, in the process, drive technology developments to improve performance or lower cost in large-scale laser optical systems. 13 refs.

  15. Structural basis for specific single-stranded RNA recognition by designer pentatricopeptide repeat proteins

    PubMed Central

    Shen, Cuicui; Zhang, Delin; Guan, Zeyuan; Liu, Yexing; Yang, Zhao; Yang, Yan; Wang, Xiang; Wang, Qiang; Zhang, QunXia; Fan, Shilong; Zou, Tingting; Yin, Ping

    2016-01-01

    As a large family of RNA-binding proteins, pentatricopeptide repeat (PPR) proteins mediate multiple aspects of RNA metabolism in eukaryotes. Binding to their target single-stranded RNAs (ssRNAs) in a modular and base-specific fashion, PPR proteins can serve as designable modules for gene manipulation. However, the structural basis for nucleotide-specific recognition by designer PPR (dPPR) proteins remains to be elucidated. Here, we report four crystal structures of dPPR proteins in complex with their respective ssRNA targets. The dPPR repeats are assembled into a right-handed superhelical spiral shell that embraces the ssRNA. Interactions between different PPR codes and RNA bases are observed at the atomic level, revealing the molecular basis for the modular and specific recognition patterns of the RNA bases U, C, A and G. These structures not only provide insights into the functional study of PPR proteins but also open a path towards the potential design of synthetic sequence-specific RNA-binding proteins. PMID:27088764

  16. Evolution of design considerations in complex craniofacial reconstruction using patient-specific implants.

    PubMed

    Peel, Sean; Bhatia, Satyajeet; Eggbeer, Dominic; Morris, Daniel S; Hayhurst, Caroline

    2016-12-01

    Previously published evidence has established major clinical benefits from using computer-aided design, computer-aided manufacturing, and additive manufacturing to produce patient-specific devices. These include cutting guides, drilling guides, positioning guides, and implants. However, custom devices produced using these methods are still not in routine use, particularly by the UK National Health Service. Oft-cited reasons for this slow uptake include the following: a higher up-front cost than conventionally fabricated devices, material-choice uncertainty, and a lack of long-term follow-up due to their relatively recent introduction. This article identifies a further gap in current knowledge - that of design rules, or key specification considerations for complex computer-aided design/computer-aided manufacturing/additive manufacturing devices. This research begins to address the gap by combining a detailed review of the literature with first-hand experience of interdisciplinary collaboration on five craniofacial patient case studies. In each patient case, bony lesions in the orbito-temporal region were segmented, excised, and reconstructed in the virtual environment. Three cases translated these digital plans into theatre via polymer surgical guides. Four cases utilised additive manufacturing to fabricate titanium implants. One implant was machined from polyether ether ketone. From the literature, articles with relevant abstracts were analysed to extract design considerations. In all, 19 frequently recurring design considerations were extracted from previous publications. Nine new design considerations were extracted from the case studies - on the basis of subjective clinical evaluation. These were synthesised to produce a design considerations framework to assist clinicians with prescribing and design engineers with modelling. Promising avenues for further research are proposed.

  17. Region and site specific design guidelines for the northeastern Venezuela development

    SciTech Connect

    Gajardo, E.; Paga, M.; Sully, J.P.

    1996-12-31

    Several site specific studies were performed for the northeastern Venezuelan offshore development in order to determine the environmental parameters for the engineering design. The geotechnical parameters were obtained from the analysis of the soil borings, in situ tests and the high resolution seismic surveys. An evaluation of the seismic hazard was done, based on a detailed neotectonic study, the compilation of historical and instrumental seismological information, and an attenuation relationship developed for the studied area. Seismic response analyses were performed using the seismic parameters and dynamic characteristics obtained form the soil borings and in situ tests of shear waves. The met-ocean design loads of the local conditions were obtained from mathematical modeling based on in situ and regional measurements. This study defines the platforms design conditions based on a probabilistic reliability approach and it has been used, among others, as a basis for the earthquake design guidelines proposed by the International Standards Organization. A sensitivity analysis was performed starting from the uncertainties of all the input parameters, in order to obtain the best estimate average design spectrum and associated coefficient of variability. The specific approach was to maintain the same safety index adopted by API RP2A code, with a bias modification factor that relates the site nominal spectra with the spectral acceleration that would be used in order to satisfy the API requirements. The results of this approach make possible the use of site specific detailed environmental data and the proven standard procedures established on the API RP2 codes, with significant cost reduction in terms of both platform and foundation design. The considerable amount of tectonic, seismological, and geotechnical information was the principal factor allowing the above methodology.

  18. Quantification of the transferability of a designed protein specificity switch reveals extensive epistasis in molecular recognition

    DOE PAGES

    Melero, Cristina; Ollikainen, Noah; Harwood, Ian; ...

    2014-10-13

    Re-engineering protein–protein recognition is an important route to dissecting and controlling complex interaction networks. Experimental approaches have used the strategy of “second-site suppressors,” where a functional interaction is inferred between two proteins if a mutation in one protein can be compensated by a mutation in the second. Mimicking this strategy, computational design has been applied successfully to change protein recognition specificity by predicting such sets of compensatory mutations in protein–protein interfaces. To extend this approach, it would be advantageous to be able to “transplant” existing engineered and experimentally validated specificity changes to other homologous protein–protein complexes. Here, we test thismore » strategy by designing a pair of mutations that modulates peptide recognition specificity in the Syntrophin PDZ domain, confirming the designed interaction biochemically and structurally, and then transplanting the mutations into the context of five related PDZ domain–peptide complexes. We find a wide range of energetic effects of identical mutations in structurally similar positions, revealing a dramatic context dependence (epistasis) of designed mutations in homologous protein–protein interactions. To better understand the structural basis of this context dependence, we apply a structure-based computational model that recapitulates these energetic effects and we use this model to make and validate forward predictions. The context dependence of these mutations is captured by computational predictions, our results both highlight the considerable difficulties in designing protein–protein interactions and provide challenging benchmark cases for the development of improved protein modeling and design methods that accurately account for the context.« less

  19. Quantification of the transferability of a designed protein specificity switch reveals extensive epistasis in molecular recognition

    SciTech Connect

    Melero, Cristina; Ollikainen, Noah; Harwood, Ian; Karpiak, Joel; Kortemme, Tanja

    2014-10-13

    Re-engineering protein–protein recognition is an important route to dissecting and controlling complex interaction networks. Experimental approaches have used the strategy of “second-site suppressors,” where a functional interaction is inferred between two proteins if a mutation in one protein can be compensated by a mutation in the second. Mimicking this strategy, computational design has been applied successfully to change protein recognition specificity by predicting such sets of compensatory mutations in protein–protein interfaces. To extend this approach, it would be advantageous to be able to “transplant” existing engineered and experimentally validated specificity changes to other homologous protein–protein complexes. Here, we test this strategy by designing a pair of mutations that modulates peptide recognition specificity in the Syntrophin PDZ domain, confirming the designed interaction biochemically and structurally, and then transplanting the mutations into the context of five related PDZ domain–peptide complexes. We find a wide range of energetic effects of identical mutations in structurally similar positions, revealing a dramatic context dependence (epistasis) of designed mutations in homologous protein–protein interactions. To better understand the structural basis of this context dependence, we apply a structure-based computational model that recapitulates these energetic effects and we use this model to make and validate forward predictions. The context dependence of these mutations is captured by computational predictions, our results both highlight the considerable difficulties in designing protein–protein interactions and provide challenging benchmark cases for the development of improved protein modeling and design methods that accurately account for the context.

  20. Crude oil specific gravity sensor design using polymer optical fiber with structural imperfection

    NASA Astrophysics Data System (ADS)

    Sulistianto, Junivan; Hatta, Agus M.; Sekartedjo, Ir.

    2016-11-01

    Crude oil is a products of oil and gas industries. One of the parameters which has in crude oil is specific gravity. In the oil and gas industries, specific gravity is used to classify crude oil which to be produced. specific gravity can be measured using ASTM D-1298 method. Disadvantage of this method is there are need of special environments and still using manual transcription. This research has been conducted a crude oil specific gravity sensor design by implementing structural imperfect to improve the performance of specific gravity sensors. In this research, polymer optical fiber is used as a sensor to measure specific gravity of crude oil. There are three variation of the amount of defect that were used in this research. Sample which used in this research has span of specific gravity value from 0,842218 to 0,9097. Based on the result, sensor with 3 imperfect structure have the best performance with linearity coefficient value 0,9438 and the span of measurement is 1,75792 dBm.

  1. Mod-5A wind turbine generator program design report. Volume 4: Drawings and specifications, book 4

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The design, development and analysis of the 7.3 MW MOD-5A wind turbine generator are documented. There are four volumes. This volume contains the drawings and specifications that were developed in preparation for building the MOD-5A wind turbine generator. This volume contains 5 books of which this is the fourth, providing drawings 47A380128 through 47A387125. In addition to the parts listing and where-used list, the logic design of the controller software and the code listing of the controller software are provided. Also given are the aerodynamic profile coordinates.

  2. Block 4 solar cell module design and test specification for residential applications

    NASA Technical Reports Server (NTRS)

    1978-01-01

    Near-term design, qualification and acceptance requirements are provided for terrestrial solar cell modules suitable for incorporation in photovoltaic power sources (2 kW to 10 kW) applied to single family residential installations. Requirement levels and recommended design limits for selected performance criteria are specified for modules intended principally for rooftop installations. Modules satisfying the requirements of this specification fall into one of two categories, residential panel or residential shingle, both meeting general performance requirements plus additional category peculiar constraints.

  3. A preliminary report of designing removable partial denture frameworks using a specifically developed software package.

    PubMed

    Han, Jing; Wang, Yong; Lü, Peijun

    2010-01-01

    This article reports on a method to digitally survey and build virtual patterns for removable partial denture (RPD) frameworks using a new three-dimensional (3D) computer-aided design/computer-assisted manufacturing (CAD/CAM) software package developed specifically for RPD design. The procedure included obtaining 3D data from partially dentate casts, deciding on the path of insertion, and modeling the shape of the components of the frameworks digitally. The completed model data were stored as stereolithography (STL) files, which are commonly used in transferring CAD/CAM models to rapid prototyping technologies. Finally, metal RPD frameworks were fabricated using a selective laser melting technique.

  4. Representing object oriented specifications and designs with extended data flow notations

    NASA Technical Reports Server (NTRS)

    Buser, Jon Franklin; Ward, Paul T.

    1988-01-01

    The issue of using extended data flow notations to document object oriented designs and specifications is discussed. Extended data flow notations, for the purposes here, refer to notations that are based on the rules of Yourdon/DeMarco data flow analysis. The extensions include additional notation for representing real-time systems as well as some proposed extensions specific to object oriented development. Some advantages of data flow notations are stated. How data flow diagrams are used to represent software objects are investigated. Some problem areas with regard to using data flow notations for object oriented development are noted. Some initial solutions to these problems are proposed.

  5. 31 CFR 800.218 - Lead agency.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 31 Money and Finance:Treasury 3 2012-07-01 2012-07-01 false Lead agency. 800.218 Section 800.218... FOREIGN PERSONS Definitions § 800.218 Lead agency. The term lead agency means an agency designated by the... activity for which the Chairperson designates it as a lead agency, including all or a portion of a...

  6. 31 CFR 800.218 - Lead agency.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 31 Money and Finance:Treasury 3 2013-07-01 2013-07-01 false Lead agency. 800.218 Section 800.218... FOREIGN PERSONS Definitions § 800.218 Lead agency. The term lead agency means an agency designated by the... activity for which the Chairperson designates it as a lead agency, including all or a portion of a...

  7. 31 CFR 800.218 - Lead agency.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 31 Money and Finance:Treasury 3 2011-07-01 2011-07-01 false Lead agency. 800.218 Section 800.218... FOREIGN PERSONS Definitions § 800.218 Lead agency. The term lead agency means an agency designated by the... activity for which the Chairperson designates it as a lead agency, including all or a portion of a...

  8. 31 CFR 800.218 - Lead agency.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 31 Money and Finance:Treasury 3 2014-07-01 2014-07-01 false Lead agency. 800.218 Section 800.218... FOREIGN PERSONS Definitions § 800.218 Lead agency. The term lead agency means an agency designated by the... activity for which the Chairperson designates it as a lead agency, including all or a portion of a...

  9. 49 CFR 178.345 - General design and construction requirements applicable to Specification DOT 406 (§ 178.346), DOT...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false General design and construction requirements... REGULATIONS SPECIFICATIONS FOR PACKAGINGS Specifications for Containers for Motor Vehicle Transportation § 178.345 General design and construction requirements applicable to Specification DOT 406 (§ 178.346),...

  10. SLS-SPEC-159 Cross-Program Design Specification for Natural Environments (DSNE) Revision D

    NASA Technical Reports Server (NTRS)

    Roberts, Barry C.

    2015-01-01

    This document is derived from the former National Aeronautics and Space Administration (NASA) Constellation Program (CxP) document CxP 70023, titled "The Design Specification for Natural Environments (DSNE), Revision C." The original document has been modified to represent updated Design Reference Missions (DRMs) for the NASA Exploration Systems Development (ESD) Programs. The DSNE completes environment-related specifications for architecture, system-level, and lower-tier documents by specifying the ranges of environmental conditions that must be accounted for by NASA ESD Programs. To assure clarity and consistency, and to prevent requirements documents from becoming cluttered with extensive amounts of technical material, natural environment specifications have been compiled into this document. The intent is to keep a unified specification for natural environments that each Program calls out for appropriate application. This document defines the natural environments parameter limits (maximum and minimum values, energy spectra, or precise model inputs, assumptions, model options, etc.), for all ESD Programs. These environments are developed by the NASA Marshall Space Flight Center (MSFC) Natural Environments Branch (MSFC organization code: EV44). Many of the parameter limits are based on experience with previous programs, such as the Space Shuttle Program. The parameter limits contain no margin and are meant to be evaluated individually to ensure they are reasonable (i.e., do not apply unrealistic extreme-on-extreme conditions). The natural environments specifications in this document should be accounted for by robust design of the flight vehicle and support systems. However, it is understood that in some cases the Programs will find it more effective to account for portions of the environment ranges by operational mitigation or acceptance of risk in accordance with an appropriate program risk management plan and/or hazard analysis process. The DSNE is not intended

  11. Facilitating interdisciplinary design specification of "smart" homes for aging in place.

    PubMed

    Demiris, George; Skubic, Marjorie; Rantz, Marilyn J; Courtney, Karen L; Aud, Myra A; Tyrer, Harry W; He, Zhihai; Lee, Jia

    2006-01-01

    "Smart homes" are defined as residences equipped with sensors and other advanced technology applications that enhance residents' independence and can be used for aging in place. The objective of this study is to determine design specifications for smart residences as defined by professional groups involved both in care delivery to senior citizens and development of devices and technologies to support aging. We assessed the importance of specific devices and sensors and their advantages and disadvantages as perceived by the interdisciplinary expert team. This work lays the ground for the implementation of smart home residencies and confirms that only an interdisciplinary design approach can address all the technical, clinical and human factors related challenges associated with home-based technologies that support aging. Our findings indicate that the use of adaptive technology that can be installed in the home environment has the potential to not only support but also empower individual senior users.

  12. Computational model design specification for Phase 1 of the Hanford Environmental Dose Reconstruction Project

    SciTech Connect

    Napier, B.A.

    1991-07-01

    The objective of the Hanford Environmental Dose Reconstruction (HEDR) Project is to estimate the radiation dose that individuals could have received as a result of emission from nuclear operations at Hanford since their inception in 1944. The purpose of this report is to outline the basic algorithm and necessary computer calculations to be used to calculate radiation doses specific and hypothetical individuals in the vicinity of Hanford. The system design requirements, those things that must be accomplished, are defined. The system design specifications, the techniques by which those requirements are met, are outlined. Included are the basic equations, logic diagrams, and preliminary definition of the nature of each input distribution. 4 refs., 10 figs., 9 tabs.

  13. Tools to aid the specification and design of flight software, appendix B

    NASA Technical Reports Server (NTRS)

    Bristow, G.

    1980-01-01

    The tasks that are normally performed during the specification and architecture design stages of software development are identified. Ways that tools could perform, or aid the performance, of such tasks are also identified. Much of the verification and analysis that is suggested is currently rarely performed during these early stages, but it is believed that this analysis should be done as early as possible so as to detect errors as early as possible.

  14. Topological optimization for designing patient-specific large craniofacial segmental bone replacements

    PubMed Central

    Sutradhar, Alok; Paulino, Glaucio H.; Miller, Michael J.; Nguyen, Tam H.

    2010-01-01

    Restoring normal function and appearance after massive facial injuries with bone loss is an important unsolved problem in surgery. An important limitation of the current methods is heuristic ad hoc design of bone replacements by the operating surgeon at the time of surgery. This problem might be addressed by incorporating a computational method known as topological optimization into routine surgical planning. We tested the feasibility of using a multiresolution three-dimensional topological optimization to design replacements for massive midface injuries with bone loss. The final solution to meet functional requirements may be shaped differently than the natural human bone but be optimized for functional needs sufficient to support full restoration using a combination of soft tissue repair and synthetic prosthetics. Topological optimization for designing facial bone tissue replacements might improve current clinical methods and provide essential enabling technology to translate generic bone tissue engineering methods into specific solutions for individual patients. PMID:20628014

  15. System specification/system design document comment review: Plutonium Stabilization and Packaging System. Notes of conference

    SciTech Connect

    1996-07-01

    A meeting was held between DOE personnel and the BNFL team to review the proposed resolutions to DOE comments on the initial issue of the system specification and system design document for the Plutonium Stabilization and Packaging System. The objectives of this project are to design, fabricate, install, and start up a glovebox system for the safe repackaging of plutonium oxide and metal, with a requirement of a 50-year storage period. The areas discussed at the meeting were: nitrogen in can; moisture instrumentation; glovebox atmosphere; can marking-bar coding; weld quality; NFPA-101 references; inner can swabbing; ultimate storage environment; throughput; convenience can screw-top design; furnace/trays; authorization basis; compactor safety; schedule for DOE review actions; fire protection; criticality safety; applicable standards; approach to MC and A; homogeneous oxide; resistance welder power; and tray overfill. Revised resolutions were drafted and are presented.

  16. Towards the formal specification of the requirements and design of a processor interface unit

    NASA Technical Reports Server (NTRS)

    Fura, David A.; Windley, Phillip J.; Cohen, Gerald C.

    1993-01-01

    Work to formally specify the requirements and design of a Processor Interface Unit (PIU), a single-chip subsystem providing memory interface, bus interface, and additional support services for a commercial microprocessor within a fault-tolerant computer system, is described. This system, the Fault-Tolerant Embedded Processor (FTEP), is targeted towards applications in avionics and space requiring extremely high levels of mission reliability, extended maintenance free operation, or both. The approaches that were developed for modeling the PIU requirements and for composition of the PIU subcomponents at high levels of abstraction are described. These approaches were used to specify and verify a nontrivial subset of the PIU behavior. The PIU specification in Higher Order Logic (HOL) is documented in a companion NASA contractor report entitled 'Towards the Formal Specification of the Requirements and Design of a Processor Interfacs Unit - HOL Listings.' The subsequent verification approach and HOL listings are documented in NASA contractor report entitled 'Towards the Formal Verification of the Requirements and Design of a Processor Interface Unit' and NASA contractor report entitled 'Towards the Formal Verification of the Requirements and Design of a Processor Interface Unit - HOL Listings.'

  17. Development of a patient specific artificial tracheal prosthesis: design, mechanical behavior analysis and manufacturing.

    PubMed

    Chua C H, Matthew; Chui, Chee Kong; Rai, Bina; Lau D P, David

    2013-01-01

    There is a need to create patient specific organ replacements as there are differences in the anatomical dimensions among individuals. High failure rates in tracheal prosthesis are attributed to the lack of mechanical strength and flexibility, slow rate of growth of ciliated epithelium and leakage of interstitial fluid into the lumen. This paper proposes a methodology of design, simulations and fabrication of a patient specific artificial tracheal prosthesis for implantation to closely mimic the biomechanical properties of the natural trachea, and describes the prototype device and its materials. Results show that the patient-specific trachea prosthesis has mechanical properties approximate that of normal tracheal rings. The user centric tracheal prosthesis is demonstrated to be a promising candidate for tracheal replacement.

  18. Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR.

    PubMed

    da Silveira Neto, Osvaldo José; Duarte, Sabrina Castilho; da Costa, Hérika Xavier; Linhares, Guido Fontgalland Coelho

    2012-01-01

    The objective of this study was to design and evaluate new primers for species-specific detection of L. infantum chagasi using PCR. Two combinations of primer pairs were established with the aim of obtaining specific amplification products from the L. infantum chagasi 18S rRNA gene. The combinations of the primer pairs and the respective sizes of the PCR products, based on the U422465 GenBank reference sequence of L. infantum chagasi, were: LCS1/LCS3 (259 bp) and LCS2/LCS3 (820 bp). It was concluded that the new PCR assays optimized using the primer pairs LCS1/LCS3 and LCS2/LCS3 were effective for specific detection of L. infantum chagasi, with analytical sensitivity to detect 1 pg/µL of DNA.

  19. Designing Specification Languages for Process Control Systems: Lessons Learned and Steps to the Future

    NASA Technical Reports Server (NTRS)

    Leveson, Nancy G.; Heimdahl, Mats P. E.; Reese, Jon Damon

    1999-01-01

    Previously, we defined a blackbox formal system modeling language called RSML (Requirements State Machine Language). The language was developed over several years while specifying the system requirements for a collision avoidance system for commercial passenger aircraft. During the language development, we received continual feedback and evaluation by FAA employees and industry representatives, which helped us to produce a specification language that is easily learned and used by application experts. Since the completion of the PSML project, we have continued our research on specification languages. This research is part of a larger effort to investigate the more general problem of providing tools to assist in developing embedded systems. Our latest experimental toolset is called SpecTRM (Specification Tools and Requirements Methodology), and the formal specification language is SpecTRM-RL (SpecTRM Requirements Language). This paper describes what we have learned from our use of RSML and how those lessons were applied to the design of SpecTRM-RL. We discuss our goals for SpecTRM-RL and the design features that support each of these goals.

  20. Engineering Bacteria to Search for Specific Concentrations of Molecules by a Systematic Synthetic Biology Design Method

    PubMed Central

    Chen, Bor-Sen

    2016-01-01

    Bacteria navigate environments full of various chemicals to seek favorable places for survival by controlling the flagella’s rotation using a complicated signal transduction pathway. By influencing the pathway, bacteria can be engineered to search for specific molecules, which has great potential for application to biomedicine and bioremediation. In this study, genetic circuits were constructed to make bacteria search for a specific molecule at particular concentrations in their environment through a synthetic biology method. In addition, by replacing the “brake component” in the synthetic circuit with some specific sensitivities, the bacteria can be engineered to locate areas containing specific concentrations of the molecule. Measured by the swarm assay qualitatively and microfluidic techniques quantitatively, the characteristics of each “brake component” were identified and represented by a mathematical model. Furthermore, we established another mathematical model to anticipate the characteristics of the “brake component”. Based on this model, an abundant component library can be established to provide adequate component selection for different searching conditions without identifying all components individually. Finally, a systematic design procedure was proposed. Following this systematic procedure, one can design a genetic circuit for bacteria to rapidly search for and locate different concentrations of particular molecules by selecting the most adequate “brake component” in the library. Moreover, following simple procedures, one can also establish an exclusive component library suitable for other cultivated environments, promoter systems, or bacterial strains. PMID:27096615

  1. MODIS Information, Data, and Control System (MIDACS) system specifications and conceptual design

    NASA Technical Reports Server (NTRS)

    Han, D.; Salomonson, V.; Ormsby, J.; Ardanuy, P.; Mckay, A.; Hoyt, D.; Jaffin, S.; Vallette, B.; Sharts, B.; Folta, D.

    1988-01-01

    The MODIS Information, Data, and Control System (MIDACS) Specifications and Conceptual Design Document discusses system level requirements, the overall operating environment in which requirements must be met, and a breakdown of MIDACS into component subsystems, which include the Instrument Support Terminal, the Instrument Control Center, the Team Member Computing Facility, the Central Data Handling Facility, and the Data Archive and Distribution System. The specifications include sizing estimates for the processing and storage capacities of each data system element, as well as traffic analyses of data flows between the elements internally, and also externally across the data system interfaces. The specifications for the data system, as well as for the individual planning and scheduling, control and monitoring, data acquisition and processing, calibration and validation, and data archive and distribution components, do not yet fully specify the data system in the complete manner needed to achieve the scientific objectives of the MODIS instruments and science teams. The teams have not yet been formed; however, it was possible to develop the specifications and conceptual design based on the present concept of EosDIS, the Level-1 and Level-2 Functional Requirements Documents, the Operations Concept, and through interviews and meetings with key members of the scientific community.

  2. 3 CFR - Designation of Officials of the Court Services and Offender Supervision Agency to Act as Director

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... improve the internal management of the executive branch and is not intended to, and does not, create any... Agency for the District of Columbia, in the order listed, shall act as and perform the functions and... become unable to perform the functions and duties of the office of the Director, until such time as...

  3. Recent developments in the design of specific Matrix Metalloproteinase inhibitors aided by structural and computational studies.

    PubMed

    Rao, B Govinda

    2005-01-01

    It has been 10 years since a 3-dimensional structure of the catalytic domain of a Matrix Metalloprotease (MMP) was revealed for the first time in 1994. More than 80 structures of different MMPs in apo and inhibited forms, determined by X-ray crystallography and NMR methods, have been published by the end of year 2003. A large number of very potent inhibitors have been disclosed in published and patent literature. Several MMP inhibitors entered clinical trials for the treatment of cancer and arthritis. Most of the first generation inhibitors have hydroxamic acid as the Zinc-binding group and have limited specificity. With the failure of these inhibitors in clinical trials, more efforts have been directed to the design of specific inhibitors with different Zn-binding groups in recent years. This review will summarize all the published structural information and focus on the inhibitors that were designed to take advantage of the nonprime side of the MMP active site using structural information and computational analysis. Representative structures from all MMPs are aligned to a target structure to provide a better understanding of the similarities and differences of the active site pockets. This analysis supports the view that the differences in the nonprime side pockets provide better opportunities for designing inhibitors with higher specificity. Published information on all the Zinc-binding groups of MMP inhibitors is reviewed for the first time. Pros and cons of inhibitors with non-hydroxamate Zinc-binding groups in terms of specificity, toxicity and pharmacokinetic properties are discussed.

  4. Earth Observatory Satellite system definition study. Report no. 5: System design and specifications. Part 2: Ground system element specifications

    NASA Technical Reports Server (NTRS)

    1974-01-01

    The Ground System requirements for the Land Resources Management (LRM) type-A and type-B missions of the Earth Observatory Satellite (EOS) program are presented. Specifications for the Thematic Mapper data processing are provided (LRM A mission). The specifications also cover the R and D instruments (Thematic Mapper and High Resolution Pointable Imager) data processing for the LRM type-B mission.

  5. Application-specific coarse-grained reconfigurable array: architecture and design methodology

    NASA Astrophysics Data System (ADS)

    Zhou, Li; Liu, Dongpei; Zhang, Jianfeng; Liu, Hengzhu

    2015-06-01

    Coarse-grained reconfigurable arrays (CGRAs) have shown potential for application in embedded systems in recent years. Numerous reconfigurable processing elements (PEs) in CGRAs provide flexibility while maintaining high performance by exploring different levels of parallelism. However, a difference remains between the CGRA and the application-specific integrated circuit (ASIC). Some application domains, such as software-defined radios (SDRs), require flexibility with performance demand increases. More effective CGRA architectures are expected to be developed. Customisation of a CGRA according to its application can improve performance and efficiency. This study proposes an application-specific CGRA architecture template composed of generic PEs (GPEs) and special PEs (SPEs). The hardware of the SPE can be customised to accelerate specific computational patterns. An automatic design methodology that includes pattern identification and application-specific function unit generation is also presented. A mapping algorithm based on ant colony optimisation is provided. Experimental results on the SDR target domain show that compared with other ordinary and application-specific reconfigurable architectures, the CGRA generated by the proposed method performs more efficiently for given applications.

  6. Structure-based design of conformation- and sequence-specific antibodies against amyloid β

    PubMed Central

    Perchiacca, Joseph M.; Ladiwala, Ali Reza A.; Bhattacharya, Moumita; Tessier, Peter M.

    2012-01-01

    Conformation-specific antibodies that recognize aggregated proteins associated with several conformational disorders (e.g., Parkinson and prion diseases) are invaluable for diagnostic and therapeutic applications. However, no systematic strategy exists for generating conformation-specific antibodies that target linear sequence epitopes within misfolded proteins. Here we report a strategy for designing conformation- and sequence-specific antibodies against misfolded proteins that is inspired by the molecular interactions governing protein aggregation. We find that grafting small amyloidogenic peptides (6–10 residues) from the Aβ42 peptide associated with Alzheimer’s disease into the complementarity determining regions of a domain (VH) antibody generates antibody variants that recognize Aβ soluble oligomers and amyloid fibrils with nanomolar affinity. We refer to these antibodies as gammabodies for grafted amyloid-motif antibodies. Gammabodies displaying the central amyloidogenic Aβ motif () are reactive with Aβ fibrils, whereas those displaying the amyloidogenic C terminus () are reactive with Aβ fibrils and oligomers (and weakly reactive with Aβ monomers). Importantly, we find that the grafted motifs target the corresponding peptide segments within misfolded Aβ conformers. Aβ gammabodies fail to cross-react with other amyloidogenic proteins and scrambling their grafted sequences eliminates antibody reactivity. Finally, gammabodies that recognize Aβ soluble oligomers and fibrils also neutralize the toxicity of each Aβ conformer. We expect that our antibody design strategy is not limited to Aβ and can be used to readily generate gammabodies against other toxic misfolded proteins. PMID:22171009

  7. Solid Waste Operations Complex W-113, Detail Design Report (Title II). Volume 3: Specifications

    SciTech Connect

    1995-09-01

    The Solid Waste Retrieval Facility--Phase 1 (Project W113) will provide the infrastructure and the facility required to retrieve from Trench 04, Burial ground 4C, contact handled (CH) drums and boxes at a rate that supports all retrieved TRU waste batching, treatment, storage, and disposal plans. This includes (1) operations related equipment and facilities, viz., a weather enclosure for the trench, retrieval equipment, weighing, venting, obtaining gas samples, overpacking, NDE, NDA, shipment of waste and (2) operations support related facilities, viz., a general office building, a retrieval staff change facility, and infrastructure upgrades such as supply and routing of water, sewer, electrical power, fire protection, roads, and telecommunication. Title I design for the operations related equipment and facilities was performed by Raytheon/BNFL, and that for the operations support related facilities including infrastructure upgrade was performed by KEH. These two scopes were combined into an integrated W113 Title II scope that was performed by Raytheon/BNFL. Volume 3 is a compilation of the construction specifications that will constitute the Title II materials and performance specifications. This volume contains CSI specifications for non-equipment related construction material type items, performance type items, and facility mechanical equipment items. Data sheets are provided, as necessary, which specify the equipment overall design parameters.

  8. Investigation into Deep Brain Stimulation Lead Designs: A Patient-Specific Simulation Study

    PubMed Central

    Alonso, Fabiola; Latorre, Malcolm A.; Göransson, Nathanael; Zsigmond, Peter; Wårdell, Karin

    2016-01-01

    New deep brain stimulation (DBS) electrode designs offer operation in voltage and current mode and capability to steer the electric field (EF). The aim of the study was to compare the EF distributions of four DBS leads at equivalent amplitudes (3 V and 3.4 mA). Finite element method (FEM) simulations (n = 38) around cylindrical contacts (leads 3389, 6148) or equivalent contact configurations (leads 6180, SureStim1) were performed using homogeneous and patient-specific (heterogeneous) brain tissue models. Steering effects of 6180 and SureStim1 were compared with symmetric stimulation fields. To make relative comparisons between simulations, an EF isolevel of 0.2 V/mm was chosen based on neuron model simulations (n = 832) applied before EF visualization and comparisons. The simulations show that the EF distribution is largely influenced by the heterogeneity of the tissue, and the operating mode. Equivalent contact configurations result in similar EF distributions. In steering configurations, larger EF volumes were achieved in current mode using equivalent amplitudes. The methodology was demonstrated in a patient-specific simulation around the zona incerta and a “virtual” ventral intermediate nucleus target. In conclusion, lead design differences are enhanced when using patient-specific tissue models and current stimulation mode. PMID:27618109

  9. Structure-based design of conformation- and sequence-specific antibodies against amyloid β.

    PubMed

    Perchiacca, Joseph M; Ladiwala, Ali Reza A; Bhattacharya, Moumita; Tessier, Peter M

    2012-01-03

    Conformation-specific antibodies that recognize aggregated proteins associated with several conformational disorders (e.g., Parkinson and prion diseases) are invaluable for diagnostic and therapeutic applications. However, no systematic strategy exists for generating conformation-specific antibodies that target linear sequence epitopes within misfolded proteins. Here we report a strategy for designing conformation- and sequence-specific antibodies against misfolded proteins that is inspired by the molecular interactions governing protein aggregation. We find that grafting small amyloidogenic peptides (6-10 residues) from the Aβ42 peptide associated with Alzheimer's disease into the complementarity determining regions of a domain (V(H)) antibody generates antibody variants that recognize Aβ soluble oligomers and amyloid fibrils with nanomolar affinity. We refer to these antibodies as gammabodies for grafted amyloid-motif antibodies. Gammabodies displaying the central amyloidogenic Aβ motif (18VFFA21) are reactive with Aβ fibrils, whereas those displaying the amyloidogenic C terminus (34LMVGGVVIA42) are reactive with Aβ fibrils and oligomers (and weakly reactive with Aβ monomers). Importantly, we find that the grafted motifs target the corresponding peptide segments within misfolded Aβ conformers. Aβ gammabodies fail to cross-react with other amyloidogenic proteins and scrambling their grafted sequences eliminates antibody reactivity. Finally, gammabodies that recognize Aβ soluble oligomers and fibrils also neutralize the toxicity of each Aβ conformer. We expect that our antibody design strategy is not limited to Aβ and can be used to readily generate gammabodies against other toxic misfolded proteins.

  10. Exploring alternate specifications to explain agency-level effects in placement decisions regarding Aboriginal children: Further analysis of the Canadian Incidence Study of Reported Child Abuse and Neglect Part C.

    PubMed

    Fallon, Barbara; Chabot, Martin; Fluke, John; Blackstock, Cindy; Sinha, Vandna; Allan, Kate; MacLaurin, Bruce

    2015-11-01

    A series of papers using data from the Canadian Incidence Study of Reported Child Abuse and Neglect (CIS) explored the influence of clinical and organizational characteristics on the decision to place Aboriginal children in out-of-home placements at the conclusion of child maltreatment investigations. The purpose of this paper is to further explore a consistent finding of the previous analyses: the proportion of investigations involving Aboriginal children at a child welfare agency is associated with placement for all children in that agency. CIS-2008 data were used in the analysis, which allowed for inclusion of previously unavailable organizational and contextual variables. Multi-level statistical models were developed to analyze the influence of clinical and organizational variables on the placement decision. Final models revealed that the proportion of investigations conducted by the child welfare agency involving Aboriginal children was again a key agency-level predictor of the placement decision for any child served by the agency. Specifically, the higher the proportion of investigations of Aboriginal children, the more likely placement was to occur for any child. Further, this analysis demonstrated that structure of governance, an organizational-level variable not available in previous cycles of the CIS, is an important agency-level predictor of out-of-home placement. Further analysis is needed to fully understand individual and organizational level variables that may influence decisions regarding placement of Aboriginal children.

  11. The design of a Phase I non site-specific Centralized Interim Storage Facility

    SciTech Connect

    Stringer, J.; Kane, D.

    1997-10-28

    The Department of Energy (DOE), Office of Civilian Radioactive Waste Management (OCRWM) recently completed a Topical Safety Analysis Report (TSAR) for a Phase 1 non site specific Centralized Interim Storage Facility (CISF). The TSAR will be used in licensing the CISF when and if a site is designated. The combined Phase 1 and Phase 2 CISF will provide federal storage capability for 40,000 metric tons of uranium (MTU) Spent Nuclear Fuel (SNF) under the oversight of the DOE. The Phase 1 TSAR was submitted to the NRC on May 1, 1997 and is currently under review having been docketed on June 10, 1997. This paper generally describes the Phase 1 CISF design and its operations as presented in the CISF TSAR.

  12. Designing a biased specification-based subjective test of image quality

    NASA Astrophysics Data System (ADS)

    Reibman, Amy R.

    2015-03-01

    Specification-based subjective tests (SBSTs) form the basis for almost all performance analysis of image and video quality estimators (QEs). Our ability to compare the efficacy of different QEs across a wide range of applications depends on careful design of these SBST, so the conclusions that are drawn about how well a QE performs are reliable and accurate. In this paper, we explore methods to design biased SBSTs for image and video QEs. A biased SBST will produce an estimate of the performance of a given QE that is systematically different than its actual performance. We demonstrate by proof of concept that it's possible to create SBSTs that generate misleading or biased Pearson or Spearman correlation coefficients between subjective and objective scores, and we present some diagnostics that begin to evaluate when influential observations have been included in the SBST. Understanding how to create biased tests is a first step toward the overall goal of creating more effective unbiased SBSTs.

  13. Optimization of affinity, specificity and function of designed influenza inhibitors using deep sequencing

    SciTech Connect

    Whitehead, Timothy A.; Chevalier, Aaron; Song, Yifan; Dreyfus, Cyrille; Fleishman, Sarel J.; De Mattos, Cecilia; Myers, Chris A.; Kamisetty, Hetunandan; Blair, Patrick; Wilson, Ian A.; Baker, David

    2012-06-19

    We show that comprehensive sequence-function maps obtained by deep sequencing can be used to reprogram interaction specificity and to leapfrog over bottlenecks in affinity maturation by combining many individually small contributions not detectable in conventional approaches. We use this approach to optimize two computationally designed inhibitors against H1N1 influenza hemagglutinin and, in both cases, obtain variants with subnanomolar binding affinity. The most potent of these, a 51-residue protein, is broadly cross-reactive against all influenza group 1 hemagglutinins, including human H2, and neutralizes H1N1 viruses with a potency that rivals that of several human monoclonal antibodies, demonstrating that computational design followed by comprehensive energy landscape mapping can generate proteins with potential therapeutic utility.

  14. An across-the-bay flight support structure designed specifically for the Spartan program

    NASA Technical Reports Server (NTRS)

    Rast, J. J.

    1986-01-01

    The preliminary design of an across-the-bay Flight Support Structure optimized to the specific requirements of the Spartan program has been completed. Although approximately 15 percent heavier than the initial goal, preliminary analysis indicate very low stress levels and significant reduction in weight should be possible in the final design. This structure is capable of supporting two Spartan 200-class spacecraft or attached payloads of equivalent total weight. The inherent structural efficiency of monocoque type construction is utilized. The minimization of fabrication and assembly costs and provision for simple and versatile payload interfacing where given significant considerations. Modularity of this structure allows it to be readily adapted for use as the primary structure of an Aries-class Spartan free-flyer utilizing the Orbit trunning latch mechanisms, thus eliminating the need for a flight support structure.

  15. Design and generation of DVD-Ig™ molecules for dual-specific targeting.

    PubMed

    DiGiammarino, Enrico; Ghayur, Tariq; Liu, Junjian

    2012-01-01

    The dual variable domain immunoglobulin (DVD-Ig™) protein is a new type of dual-specific IgG. As a novel therapeutic class, the great potential of the DVD-Ig protein is to simultaneously target two mediators of disease by a single pharmaceutical entity. The molecule contains an Fc region and constant regions in a configuration similar to a conventional IgG; however, the DVD-Ig protein is unique in that each arm of the molecule contains two variable domains (VDs). The VDs within an arm are linked in tandem and can possess different binding specificities. Here, we discuss critical design features of the DVD-Ig protein and describe a methodology for cloning, expressing, and purifying the molecules.

  16. Design and specification of a centralized manufacturing data management and scheduling system

    NASA Technical Reports Server (NTRS)

    Farrington, Phillip A.

    1993-01-01

    As was revealed in a previous study, the Materials and Processes Laboratory's Productivity Enhancement Complex (PEC) has a number of automated production areas/cells that are not effectively integrated, limiting the ability of users to readily share data. The recent decision to utilize the PEC for the fabrication of flight hardware has focused new attention on the problem and brought to light the need for an integrated data management and scheduling system. This report addresses this need by developing preliminary designs specifications for a centralized manufacturing data management and scheduling system for managing flight hardware fabrication in the PEC. This prototype system will be developed under the auspices of the Integrated Engineering Environment (IEE) Oversight team and the IEE Committee. At their recommendation the system specifications were based on the fabrication requirements of the AXAF-S Optical Bench.

  17. Degenerate PCR primer design for the specific identification of rhinovirus C.

    PubMed

    Nam, Young Ran; Lee, Uk; Choi, Han Seok; Lee, Kyoung Jin; Kim, Nari; Jang, Yong Ju; Joo, Chul Hyun

    2015-03-01

    Human rhinovirus (HRV)-A and -B is a common cause of upper respiratory tract infections. Recently, a third species, HRV-C, was categorized based on molecular typing studies. The results showed that the HRV-C genome had diverged from that of HRV-A and -B. Despite its late identification, increasing evidence suggests that HRV-C causes more severe pathogenic infections than HRV-A or -B; however, a large amount of epidemiological data is required to confirm this association in different clinical settings. Consequently, a simple and rapid method for identifying HRV-C is required to expedite such epidemiological studies. Here, two degenerate primer sets (HEV and HRVC) were designed based on bioinformatic analyses. The HEV set targeting the fifth IRES domain sequence within the 5'-UTR, which is highly conserved among enteroviruses, was designed to detect all enteroviruses, whereas the HRVC set, which targeted the VP2 coding region, was designed to detect HRV-C alone. Both primer sets were tested against a panel of standard enteroviruses and clinical lavage samples. HEV detected all enteroviruses tested whereas HRVC was specific for HRV-C. Although the primer design strategy was confirmed with a limited number of samples, extensive tests are required to be applied in clinical settings.

  18. Design of a High Temperature Radiator for the Variable Specific Impulse Magnetoplasma Rocket

    NASA Technical Reports Server (NTRS)

    Sheth, Rubik B.; Ungar, Eugene K.; Chambliss, Joe P.

    2012-01-01

    The Variable Specific Impulse Magnetoplasma Rocket (VASIMR), currently under development by Ad Astra Rocket Company (Webster, TX), is a unique propulsion system that could change the way space propulsion is performed. VASIMR's efficiency, when compared to that of a conventional chemical rocket, reduces the propellant needed for exploration missions by a factor of 10. Currently plans include flight tests of a 200 kW VASIMR system, titled VF-200, on the International Space Station (ISS). The VF-200 will consist of two 100 kW thruster units packaged together in one engine bus. Each thruster core generates 27 kW of waste heat during its 15 minute firing time. The rocket core will be maintained between 283 and 573 K by a pumped thermal control loop. The design of a high temperature radiator is a unique challenge for the vehicle design. This paper will discuss the path taken to develop a steady state and transient-based radiator design. The paper will describe the radiator design option selected for the VASIMR thermal control system for use on ISS, and how the system relates to future exploration vehicles.

  19. Design Analysis of a High Temperature Radiator for the Variable Specific Impulse Magnetoplasma Rocket (VASIMR)

    NASA Technical Reports Server (NTRS)

    Sheth, Rubik B.; Ungar, Eugene K.; Chambliss, Joe P.; Cassady, Leonard D.

    2011-01-01

    The Variable Specific Impulse Magnetoplasma Rocket (VASIMR), currently under development by Ad Astra Rocket Company, is a unique propulsion system that can potentially change the way space propulsion is performed. VASIMR's efficiency, when compared to that of a conventional chemical rocket, reduce propellant needed for exploration missions by a factor of 10. Currently plans include flight tests of a 200 kW VASIMR system, titled VF-200, on the International Space Station. The VF-200 will consist of two 100 kW thruster units packaged together in one engine bus. Each thruster unit has a unique heat rejection requirement of about 27 kW over a firing time of 15 minutes. In order to control rocket core temperatures, peak operating temperatures of about 300 C are expected within the thermal control loop. Design of a high temperature radiator is a unique challenge for the vehicle design. This paper will discuss the path taken to develop a steady state and transient based radiator design. The paper will describe radiator design options for the VASIMR thermal control system for use on ISS as well as future exploration vehicles.

  20. Mod-5A wind turbine generator program design report. Volume 4: Drawings and specifications, book 2

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The design, development and analysis of the 7.3 MW MOD-5A wind turbine generator is documented. There are four volumes. This volume contains the drawings and specifications that were developed in preparation for building the MOD-5A wind turbine generator. This is the second book of volume four. Some of the items it contains are specs for the emergency shutdown panel, specs for the simulator software, simulator hardware specs, site operator terminal requirements, control data system requirements, software project management plan, elastomeric teeter bearing requirement specs, specs for the controls electronic cabinet, and specs for bolt pretensioning.

  1. Application of Fraunhofer diffraction theory to feature-specific detector design.

    PubMed

    Hardy, J A; Wheeless, L L

    1977-07-01

    Light scatter from epithelial cells in a slit-scan flow system is modeled using the Fraunhofer condition of scalar diffraction theory. Power spectra are calculated for successive positions of model cells in the line focus of a laser beam with a Fourier transform computer program. Using the calculated power spectra, detector configurations are designed to detect specific cell structures of interest. Detector configurations are tested in a static slit-scan scatter apparatus. Data indicating the ability to detect boundaries and cell orientation are discussed.

  2. Target design considerations for high specific activity [{sup 11}C]O{sub 2}

    SciTech Connect

    Ferrieri, R.A.; Alexoff, D.L.; Schlyer, D.J.; McDonald, K.; Wolf, A.P.

    1993-12-31

    In the routine preparation of {sup 11}C-labeled compounds through N-[{sup 11}C]-methylation using [{sup 11}C]H{sub 3}I, total masses are always higher than synthesis mass contribution, suggesting that the target system contributes carrier carbon to the final product mass. This conclusion prompted this evaluation of target materials and target design for [{sup 11}C]O{sub 2} production. Ultimately, one is faced with the sprospect of compromising between [{sup 11}C]O{sub 2} specific activity and the amount that can be extracted from the target after a reasonable irradiation time.

  3. Mod-5A wind turbine generator program design report. Volume 4: Drawings and specifications, book 2

    NASA Astrophysics Data System (ADS)

    1984-08-01

    The design, development and analysis of the 7.3 MW MOD-5A wind turbine generator is documented. There are four volumes. This volume contains the drawings and specifications that were developed in preparation for building the MOD-5A wind turbine generator. This is the second book of volume four. Some of the items it contains are specs for the emergency shutdown panel, specs for the simulator software, simulator hardware specs, site operator terminal requirements, control data system requirements, software project management plan, elastomeric teeter bearing requirement specs, specs for the controls electronic cabinet, and specs for bolt pretensioning.

  4. Acoustic specifications for the design of jet engine test facilities on an airbase

    SciTech Connect

    Strumpf, F.M.

    1982-01-01

    The use of engine run up test arrangements was common in Israeli air-bases since the forties, when engines for the Mustang, Mosquito, Harward and other propellor powered planes were used. The era of jet engine propulsion boosted the noise levels, and the use of fighters with afterburners in the new engines of the 80's brought it up to unbearable levels. Thus, the growth of the Israeli Air Force demanded the use of efficient noise suppression devices. These were divided into engine run-up noise suppressors, and aircraft noise suppessors (Hush Houses). For both of the bove ground arrangements, acoustic specifications had to be given. They were, as well as design goals for the manufacturers, also needed to restrict noise levels on the air-base as well as its surroundings. The acoustic specifications discussed are based on measured data, and permitted noise levels in the homes on the base being as far as 2500 meters from the engine exhaust silencer. For the special air-base discussed, various criteria were tested, including US Military Specifications, none of which were acceptable, and a special specification was therefore prepared.

  5. Design of Patient-Specific Gait Modifications for Knee Osteoarthritis Rehabilitation

    PubMed Central

    Fregly, Benjamin J.; Reinbolt, Jeffrey A.; Rooney, Kelly L.; Mitchell, Kim H.; Chmielewski, Terese L.

    2007-01-01

    Gait modification is a nonsurgical approach for reducing the external knee adduction torque in patients with knee osteoarthritis (OA). The magnitude of the first adduction torque peak in particular is strongly associated with knee OA progression. While toeing out has been shown to reduce the second peak, no clinically realistic gait modifications have been identified that effectively reduce both peaks simultaneously. This study predicts novel patient-specific gait modifications that achieve this goal without changing the foot path. The modified gait motion was designed for a single patient with knee OA using dynamic optimization of a patient-specific, full-body gait model. The cost function minimized the knee adduction torque subject to constraints limiting how much the new gait motion could deviate from the patient's normal gait motion. The optimizations predicted a “medial-thrust” gait pattern that reduced the first adduction torque peak between 32% and 54% and the second peak between 34% and 56%. The new motion involved three synergistic kinematic changes: slightly decreased pelvis obliquity, slightly increased leg flexion, and slightly increased pelvis axial rotation. After gait retraining, the patient achieved adduction torque reductions of 39% to 50% in the first peak and 37% to 55% in the second one. These reductions are comparable to those reported after high tibial osteotomy surgery. The associated kinematic changes were consistent with the predictions except for pelvis obliquity, which showed little change. This study demonstrates that it is feasible to design novel patient-specific gait modifications with potential clinical benefit using dynamic optimization of patient-specific, full-body gait models. Further investigation is needed to assess the extent to which similar gait modifications may be effective for other patients with knee OA. PMID:17867361

  6. Power Supply System for the Atlas Experiment: Design Specifications, Implementation, Test and First Results

    NASA Astrophysics Data System (ADS)

    Lazzaroni, M.; Citterio, M.; Latorre, S.; Lanza, A.; Cova, P.; Delmonte, N.; Giuliani, F.

    2014-06-01

    The planned upgrade of instrumentation sensitivity in the ATLAS experiment of the Large Hadron Collider (LHC), at CERN, calls for a new type of power distribution architecture. Moreover, power supplies require DC-DC power converters able to work in very hostile environment and maintaining high level of Reliability, Availability, Maintainability and Safety (denoted as RAMS requirements) during the experimental activity. Two main issues need to be discussed: first, electronic devices and equipment must operate in very high background of both charged and neutral particles and high static magnetic field and, second, the increase of the radiation background and the requirements of new front-end electronics are indeed incompatible with the current capability of the actual distribution system. The APOLLO R&D collaboration, funded by the Italian Istituto Nazionale di Fisica Nucleare (INFN), aims to study dedicated topologies of both distribution system and DC-DC power converters and to design, build and test demonstrators, developing the needed technology for the industrialization phase. The collaboration has designed a 3kW, 280V-12V converter (MC) based on the Switch in Line architecture (SIL), a DC to DC phase-shifted converter characterized by a disposition in line of the MOSFETs with good soft switching performances, and in the last year many steps have been taken to enhance the power dissipation and the reliability and to improve the general features of the designed converter. In particular a new water heat sink was designed on the basis of TFD simulation accounting for the layout of the specific converter. Experimental activities in order to characterize both thermal and electrical features of the MC confirm the correctness of the adopted design criteria.

  7. Molecular Design of Antifouling Polymer Brushes Using Sequence-Specific Peptoids

    DOE PAGES

    Lau, King Hang Aaron; Sileika, Tadas S.; Park, Sung Hyun; ...

    2014-11-26

    Material systems that can be used to flexibly and precisely define the chemical nature and molecular arrangement of a surface would be invaluable for the control of complex biointerfacial interactions. For example, progress in antifouling polymer biointerfaces that prevents nonspecific protein adsorption and cell attachment, which can significantly improve the performance of an array of biomedical and industrial applications, is hampered by a lack of chemical models to identify the molecular features conferring their properties. Poly(N-substituted glycine) “peptoids” are peptidomimetic polymers that can be conveniently synthesized with specific monomer sequences and chain lengths, and are presented as a versatile platformmore » for investigating the molecular design of antifouling polymer brushes. Zwitterionic antifouling polymer brushes have captured significant recent attention, and a targeted library of zwitterionic peptoid brushes with different charge densities, hydration, separations between charged groups, chain lengths, and grafted chain densities, is quantitatively evaluated for their antifouling properties through a range of protein adsorption and cell attachment assays. Specific zwitterionic brush designs are found to give rise to distinct but subtle differences in properties. In conclusion, the results also point to the dominant roles of the grafted chain density and chain length in determining the performance of antifouling polymer brushes.« less

  8. Molecular Design of Antifouling Polymer Brushes Using Sequence-Specific Peptoids

    SciTech Connect

    Lau, King Hang Aaron; Sileika, Tadas S.; Park, Sung Hyun; Sousa, Ana M. L.; Burch, Patrick; Szleifer, Igal; Messersmith, Phillip B.

    2014-11-26

    Material systems that can be used to flexibly and precisely define the chemical nature and molecular arrangement of a surface would be invaluable for the control of complex biointerfacial interactions. For example, progress in antifouling polymer biointerfaces that prevents nonspecific protein adsorption and cell attachment, which can significantly improve the performance of an array of biomedical and industrial applications, is hampered by a lack of chemical models to identify the molecular features conferring their properties. Poly(N-substituted glycine) “peptoids” are peptidomimetic polymers that can be conveniently synthesized with specific monomer sequences and chain lengths, and are presented as a versatile platform for investigating the molecular design of antifouling polymer brushes. Zwitterionic antifouling polymer brushes have captured significant recent attention, and a targeted library of zwitterionic peptoid brushes with different charge densities, hydration, separations between charged groups, chain lengths, and grafted chain densities, is quantitatively evaluated for their antifouling properties through a range of protein adsorption and cell attachment assays. Specific zwitterionic brush designs are found to give rise to distinct but subtle differences in properties. In conclusion, the results also point to the dominant roles of the grafted chain density and chain length in determining the performance of antifouling polymer brushes.

  9. Multivalent drug design and inhibition of cholera toxin by specific and transient protein-ligand interactions.

    PubMed

    Liu, Jiyun; Begley, Darren; Mitchell, Daniel D; Verlinde, Christophe L M J; Varani, Gabriele; Fan, Erkang

    2008-05-01

    Multivalent inhibitors of the cholera toxin B pentamer are potential therapeutic drugs for treating cholera and serve as models for demonstrating multivalent ligand effects through a structure-based approach. A crucial yet often overlooked aspect of multivalent drug design is the length, rigidity and chemical composition of the linker used to connect multiple binding moieties. To specifically study the role of chemical linkers in multivalent ligand design, we have synthesized a series of compounds with one and two binding motifs connected by several different linkers. These compounds have affinity for and potency against the cholera toxin B pentamer despite the fact that none can simultaneously bind two toxin receptor sites. Results from saturation transfer difference NMR reveal transient, non-specific interactions between the cholera toxin and linker groups contribute significantly to overall binding affinity of monovalent compounds. However, the same random protein-ligand interactions do not appear to affect binding of bivalent molecules. Moreover, the binding affinities and potencies of these 'non-spanning' bivalent ligands appear to be wholly independent of linker length. Our detailed analysis identifies multiple effects that account for the improved inhibitory potencies of bivalent ligands and suggest approaches to further improve the activity of this class of compounds.

  10. A Symmetrical, Planar SOFC Design for NASA's High Specific Power Density Requirements

    NASA Technical Reports Server (NTRS)

    Cable, Thomas L.; Sofie, Stephen W.

    2007-01-01

    Solid oxide fuel cell (SOFC) systems for aircraft applications require an order of magnitude increase in specific power density (1.0 kW/kg) and long life. While significant research is underway to develop anode supported cells which operate at temperatures in the range of 650-800 C, concerns about Cr-contamination from the metal interconnect may drive the operating temperature down further, to 750 C and lower. Higher temperatures, 900-1000 C, are more favorable for SOFC stacks to achieve specific power densities of 1.0 kW/kg. Since metal interconnects are not practical at these high temperatures and can account for up to 75% of the weight of the stack, NASA is pursuing a design that uses a thin, LaCrO3-based ceramic interconnect that incorporates gas channels into the electrodes. The bi-electrode supported cell (BSC) uses porous YSZ scaffolds, on either side of a 10-20 microns electrolyte. The porous support regions are fabricated with graded porosity using the freeze-tape casting process which can be tailored for fuel and air flow. Removing gas channels from the interconnect simplifies the stack design and allows the ceramic interconnect to be kept thin, on the order of 50 -100 microns. The YSZ electrode scaffolds are infiltrated with active electrode materials following the high temperature sintering step. The NASA-BSC is symmetrical and CTE matched, providing balanced stresses and favorable mechanical properties for vibration and thermal cycling.

  11. A multi-agency sutrient dataset used to estimate loads, improve monitoring design, and calibrate regional sutrient SPARROW models

    USGS Publications Warehouse

    Saad, David A.; Schwarz, Gregory; Robertson, Dale M.; Booth, Nathaniel

    2011-01-01

    Stream-loading information was compiled from federal, state, and local agencies, and selected universities as part of an effort to develop regional SPAtially Referenced Regressions On Watershed attributes (SPARROW) models to help describe the distribution, sources, and transport of nutrients in streams throughout much of the United States. After screening, 2,739 sites, sampled by 73 agencies, were identified as having suitable data for calculating long-term mean annual nutrient loads required for SPARROW model calibration. These sites had a wide range in nutrient concentrations, loads, and yields, and environmental characteristics in their basins. An analysis of the accuracy in load estimates relative to site attributes indicated that accuracy in loads improve with increases in the number of observations, the proportion of uncensored data, and the variability in flow on observation days, whereas accuracy declines with increases in the root mean square error of the water-quality model, the flow-bias ratio, the number of days between samples, the variability in daily streamflow for the prediction period, and if the load estimate has been detrended. Based on compiled data, all areas of the country had recent declines in the number of sites with sufficient water-quality data to compute accurate annual loads and support regional modeling analyses. These declines were caused by decreases in the number of sites being sampled and data not being entered in readily accessible databases.

  12. The Effects of Goal Specificity and Scaffolding on Programming Performance and Self-Regulation in Game Design

    ERIC Educational Resources Information Center

    Feng, Chia-Yen; Chen, Ming-Puu

    2014-01-01

    The purpose of this study was to investigate the influence of goal specificity and scaffolding on the programming performance and self-regulation of elementary students engaged in learning game design. This study recruited 232 students for the experimental activities. Two levels of goal specificity were employed: specific and nonspecific.…

  13. Design and evaluation of matrices of Eudragit with polycarbophil and carbopol for colon-specific delivery.

    PubMed

    Asghar, Laila Fatima Ali; Chandran, Sajeev

    2008-12-01

    The purpose of the present study was to investigate the effect of incorporating pH-responsive polymers Eudragit (L100 or S100) in matrix bases composed of hydrophilic polymers polycarbophil and carbopol to design oral controlled release formulations with sigmoidal release profile for colon-specific delivery. Matrix tablets were prepared by wet granulation technique using indomethacin as model drug and were characterized for physical parameters, in vitro drug release, release kinetics, and stability on storage. The gastrointestinal (GI) transit of selected formulations was also investigated in human subjects using gamma scintigraphy. In vitro release studies indicated that the presence of pH-sensitive polymers in hydrophilic polymer base retarded the initial release significantly (10-15% release in 6 h) followed with controlled release for the next 8-10 h in simulated GI fluid pH (without enzymes). The presence of Eudragit in hydrophilic matrix base retarded the swelling of the matrix base in acidic to weakly acidic pH, but in alkaline pH, enhancement in drug release rate was observed due to the dissolution of Eudragit from the base resulting in a porous matrix structure, resulting in around 80-90% release in 14 h of study. In vivo gamma scintigraphy studies in healthy human subjects proved that the formulations had acceptable matrix strength to withstand gastric and colonic transit. The mean colonic residence time of selected designed formulations varied between 15 and 19 h. Such a matrix design could have potential application as colon-specific drug delivery systems with pH- and time-dependent drug release profile.

  14. Specific noncovalent interactions at protein-ligand interface: implications for rational drug design.

    PubMed

    Zhou, P; Huang, J; Tian, F

    2012-01-01

    Specific noncovalent interactions that are indicative of attractive, directional intermolecular forces have always been of key interest to medicinal chemists in their search for the "glue" that holds drugs and their targets together. With the rapid increase in the number of solved biomolecular structures as well as the performance enhancement of computer hardware and software in recent years, it is now possible to give more comprehensive insight into the geometrical characteristics and energetic landscape of certain sophisticated noncovalent interactions present at the binding interface of protein receptors and small ligands based on accumulated knowledge gaining from the combination of two quite disparate but complementary approaches: crystallographic data analysis and quantum-mechanical ab initio calculation. In this perspective, we survey massive body of published works relating to structural characterization and theoretical investigation of three kinds of strong, specific, direct, enthalpy-driven intermolecular forces, including hydrogen bond, halogen bond and salt bridge, involved in the formation of protein-ligand complex architecture in order to characterize their biological functions in conferring affinity and specificity for ligand recognition by host protein. In particular, the biomedical implications of raised knowledge are discussed with respect to potential applications in rational drug design.

  15. Engineering the Pseudomonas aeruginosa II lectin: designing mutants with changed affinity and specificity.

    PubMed

    Kříž, Zdeněk; Adam, Jan; Mrázková, Jana; Zotos, Petros; Chatzipavlou, Thomais; Wimmerová, Michaela; Koča, Jaroslav

    2014-09-01

    This article focuses on designing mutations of the PA-IIL lectin from Pseudomonas aeruginosa that lead to change in specificity. Following the previous results revealing the importance of the amino acid triad 22-23-24 (so-called specificity-binding loop), saturation in silico mutagenesis was performed, with the intent of finding mutations that increase the lectin's affinity and modify its specificity. For that purpose, a combination of docking, molecular dynamics and binding free energy calculation was used. The combination of methods revealed mutations that changed the performance of the wild-type lectin and its mutants to their preferred partners. The mutation at position 22 resulted in 85% in inactivation of the binding site, and the mutation at 23 did not have strong effects thanks to the side chain being pointed away from the binding site. Molecular dynamics simulations followed by binding free energy calculation were performed on mutants with promising results from docking, and also at those where the amino acid at position 24 was replaced for bulkier or longer polar chain. The key mutants were also prepared in vitro and their binding properties determined by isothermal titration calorimetry. Combination of the used methods proved to be able to predict changes in the lectin performance and helped in explaining the data observed experimentally.

  16. Design of Deinococcus radiodurans thioredoxin reductase with altered thioredoxin specificity using computational alanine mutagenesis.

    PubMed

    Obiero, Josiah; Sanders, David A R

    2011-06-01

    In this study, the X-ray crystal structure of the complex between Escherichia coli thioredoxin reductase (EC TrxR) and its substrate thioredoxin (Trx) was used as a guide to design a Deinococcus radiodurans TrxR (DR TrxR) mutant with altered Trx specificity. Previous studies have shown that TrxRs have higher affinity for cognate Trxs (same species) than that for Trxs from different species. Computational alanine scanning mutagenesis and visual inspection of the EC TrxR-Trx interface suggested that only four residues (F81, R130, F141, and F142) account for the majority of the EC TrxR-Trx interface stability. Individual replacement of equivalent residues in DR TrxR (M84, K137, F148, and F149) with alanine resulted in drastic changes in binding affinity, confirming that the four residues account for most of TrxR-Trx interface stability. When M84 and K137 were changed to match equivalent EC TrxR residues (K137R and M84F), the DR TrxR substrate specificity was altered from its own Trx to that of EC Trx. The results suggest that a small subset of the TrxR-Trx interface residues is responsible for the majority of Trx binding affinity and species-specific recognition.

  17. Earth Observatory Satellite system definition study. Report 5: System design and specifications. Volume 3: General purpose spacecraft segment and module specifications

    NASA Technical Reports Server (NTRS)

    1974-01-01

    The specifications for the Earth Observatory Satellite (EOS) general purpose aircraft segment are presented. The satellite is designed to provide attitude stabilization, electrical power, and a communications data handling subsystem which can support various mission peculiar subsystems. The various specifications considered include the following: (1) structures subsystem, (2) thermal control subsystem, (3) communications and data handling subsystem module, (4) attitude control subsystem module, (5) power subsystem module, and (6) electrical integration subsystem.

  18. 77 FR 69507 - Proposed Model Safety Evaluation for Plant-Specific Adoption of Technical Specifications Task...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-19

    ... COMMISSION Proposed Model Safety Evaluation for Plant-Specific Adoption of Technical Specifications Task... Designs'' AGENCY: Nuclear Regulatory Commission. ACTION: Notice of opportunity for public comment. SUMMARY... evaluation (SE) for plant- specific adoption of Technical Specifications (TS) Task Force (TSTF) Traveler...

  19. Outcome in design-specific comparisons between highly crosslinked and conventional polyethylene in total hip arthroplasty.

    PubMed

    Johanson, Per-Erik; Furnes, Ove; Ivar Havelin, Leif; Fenstad, Anne Marie; Pedersen, Alma B; Overgaard, Søren; Garellick, Göran; Mäkelä, Keijo; Kärrholm, Johan

    2017-04-04

    Background and purpose - Most registry studies regarding highly crosslinked polyethylene (XLPE) have focused on the overall revision risk. We compared the risk of cup and/or liner revision for specific cup and liner designs made of either XLPE or conventional polyethylene (CPE), regarding revision for any reason and revision due to aseptic loosening and/or osteolysis. Patients and methods - Using the Nordic Arthroplasty Register Association (NARA) database, we identified cup and liner designs where either XLPE or CPE had been used in more than 500 THAs performed for primary hip osteoarthritis. We assessed risk of revision for any reason and for aseptic loosening using Cox regression adjusted for age, sex, femoral head material and size, surgical approach, stem fixation, and presence of hydroxyapatite coating (uncemented cups). Results - The CPE version of the ZCA cup had a risk of revision for any reason similar to that of the XLPE version (p = 0.09), but showed a 6-fold higher risk of revision for aseptic loosening (p < 0.001). The CPE version of the Reflection All Poly cup had an 8-fold elevated risk of revision for any reason (p < 0.001) and a 5-fold increased risk of revision for aseptic loosening (p < 0.001). The Charnley Elite Ogee/Marathon cup and the Trilogy cup did not show such differences. Interpretation - Whether XLPE has any advantage over CPE regarding revision risk may depend on the properties of the polyethylene materials being compared, as well as the respective cup designs, fixation type, and follow-up times. Further research is needed to elucidate how cup design factors interact with polyethylene type to affect the risk of revision.

  20. Design and evaluation of Bacteroides DNA probes for the specific detection of human fecal pollution

    SciTech Connect

    Kreader, C.A.

    1995-04-01

    Because Bacteroides spp. are obligate anaerobes that dominate the human fecal flora, and because some species may live only in the human intestine, these bacteria might be useful to distinguish human from nonhuman sources of fecal pollution. To test this hypothesis, PCR primers specific for 16S rRNA gene sequences of Bacteroides distasonis, B. thetaiotaomicron, and B. vulgatus were designed. Hybridization with species-specific internal probes was used to detect the intended PCR products. Extracts from 66 known Bacteroides strains, representing 10 related species, were used to confirm the specificity of these PCR-hybridization assays. To test for specificity in feces, procedures were developed to prepare DNA of sufficient purity for PCR. Extracts of feces from 9 humans and 70 nonhumans (cats, dogs, cattle, hogs, horses, sheep, goats, and chickens) were each analyzed with and without an internal positive control to verify that PCR amplification was not inhibited by substances in the extract. In addition, serial dilutions from each extract that tested positive were assayed to estimate the relative abundance of target Bacteroides spp. in the sample. Depending on the primer-probe set used, either 78 or 67% of the human fecal extracts tested had high levels of target DNA. On the other hand, only 7 to 11% of the nonhuman extracts tested had similarly high levels of target DNA. An additional 12 to 20% of the nonhuman extracts had levels of target DNA that were 100- to 1,000-fold lower than those found in humans. Although the B. vulgatus probes detected high levels of their target DNA in most of the house pets, similarly high levels of target DNA were found only in a few individuals from other groups of nonhumans. Therefore, the results indicate that these probes can distinguish human from non human feces in many cases. 50 refs., 5 figs., 2 tabs.

  1. Earth Observatory Satellite system definition study. Report 5: System design and specifications. Volume 2: EOS-A system specification

    NASA Technical Reports Server (NTRS)

    1974-01-01

    The objectives of the Earth Observatory Satellite (EOS) program are defined. The system specifications for the satellite payload are examined. The broad objectives of the EOS-A program are as follows: (1) to develop space-borne sensors for the measurement of land resources, (2) to evolve spacecraft systems and subsystems which will permit earth observation with greater accuracy, coverage, spatial resolution, and continuity than existing systems, (3) to develop improved information processing, extraction, display, and distribution systems, and (4) to use space transportation systems for resupply and retrieval of the EOS.

  2. A Multi-Agency Nutrient Dataset Used to Estimate Loads, Improve Monitoring Design, and Calibrate Regional Nutrient SPARROW Models

    USGS Publications Warehouse

    Saad, D.A.; Schwarz, G.E.; Robertson, D.M.; Booth, N.L.

    2011-01-01

    Stream-loading information was compiled from federal, state, and local agencies, and selected universities as part of an effort to develop regional SPAtially Referenced Regressions On Watershed attributes (SPARROW) models to help describe the distribution, sources, and transport of nutrients in streams throughout much of the United States. After screening, 2,739 sites, sampled by 73 agencies, were identified as having suitable data for calculating long-term mean annual nutrient loads required for SPARROW model calibration. These sites had a wide range in nutrient concentrations, loads, and yields, and environmental characteristics in their basins. An analysis of the accuracy in load estimates relative to site attributes indicated that accuracy in loads improve with increases in the number of observations, the proportion of uncensored data, and the variability in flow on observation days, whereas accuracy declines with increases in the root mean square error of the water-quality model, the flow-bias ratio, the number of days between samples, the variability in daily streamflow for the prediction period, and if the load estimate has been detrended. Based on compiled data, all areas of the country had recent declines in the number of sites with sufficient water-quality data to compute accurate annual loads and support regional modeling analyses. These declines were caused by decreases in the number of sites being sampled and data not being entered in readily accessible databases. ?? 2011 American Water Resources Association. This article is a U.S. Government work and is in the public domain in the USA.

  3. Detailed design specification for the ALT Shuttle Information Extraction Subsystem (SIES)

    NASA Technical Reports Server (NTRS)

    Clouette, G. L.; Fitzpatrick, W. N.

    1976-01-01

    The approach and landing test (ALT) shuttle information extraction system (SIES) is described in terms of general requirements and system characteristics output products and processing options, output products and data sources, and system data flow. The ALT SIES is a data reduction system designed to satisfy certain data processing requirements for the ALT phase of the space shuttle program. The specific ALT SIES data processing requirements are stated in the data reduction complex approach and landing test data processing requirements. In general, ALT SIES must produce time correlated data products as a result of standardized data reduction or special purpose analytical processes. The main characteristics of ALT SIES are: (1) the system operates in a batch (non-interactive) mode; (2) the processing is table driven; (3) it is data base oriented; (4) it has simple operating procedures; and (5) it requires a minimum of run time information.

  4. Probing the surface of human carbonic anhydrase for clues towards the design of isoform specific inhibitors.

    PubMed

    Pinard, Melissa A; Mahon, Brian; McKenna, Robert

    2015-01-01

    The alpha carbonic anhydrases (α-CAs) are a group of structurally related zinc metalloenzymes that catalyze the reversible hydration of CO2 to HCO3(-). Humans have 15 different α-CAs with numerous physiological roles and expression patterns. Of these, 12 are catalytically active, and abnormal expression and activities are linked with various diseases, including glaucoma and cancer. Hence there is a need for CA isoform specific inhibitors to avoid off-target CA inhibition, but due to the high amino acid conservation of the active site and surrounding regions between each enzyme, this has proven difficult. However, residues towards the exit of the active site are variable and can be exploited to design isoform selective inhibitors. Here we discuss and characterize this region of "selective drug targetability" and how these observations can be utilized to develop isoform selective CA inhibitors.

  5. Signal processing and display interface studies. [performance tests - design analysis/equipment specifications

    NASA Technical Reports Server (NTRS)

    1975-01-01

    Signal processing equipment specifications, operating and test procedures, and systems design and engineering are described. Five subdivisions of the overall circuitry are treated: (1) the spectrum analyzer; (2) the spectrum integrator; (3) the velocity discriminator; (4) the display interface; and (5) the formatter. They function in series: (1) first in analog form to provide frequency resolution, (2) then in digital form to achieve signal to noise improvement (video integration) and frequency discrimination, and (3) finally in analog form again for the purpose of real-time display of the significant velocity data. The formatter collects binary data from various points in the processor and provides a serial output for bi-phase recording. Block diagrams are used to illustrate the system.

  6. Subcontract Report: Modular Combined Heat & Power System for Utica College: Design Specification

    SciTech Connect

    Rouse, Greg

    2007-09-01

    Utica College, located in Utica New York, intends to install an on-site power/cogeneration facility. The energy facility is to be factory pre-assembled, or pre- assembled in modules, to the fullest extent possible, and ready to install and interconnect at the College with minimal time and engineering needs. External connections will be limited to fuel supply, electrical output, potable makeup water as required and cooling and heat recovery systems. The proposed facility will consist of 4 self-contained, modular Cummins 330kW engine generators with heat recovery systems and the only external connections will be fuel supply, electrical outputs and cooling and heat recovery systems. This project was eventually cancelled due to changing DOE budget priorities, but the project engineers produced this system design specification in hopes that it may be useful in future endeavors.

  7. Designed auto-assembly of nanostreptabodies for rapid tissue-specific targeting in vivo.

    PubMed

    Valadon, Philippe; Darsow, Bryan; Buss, Tim N; Czarny, Malgorzata; Griffin, Noelle M; Nguyen, Han N; Oh, Phil; Borgstrom, Per; Chrastina, Adrian; Schnitzer, Jan E

    2010-01-01

    Molecular medicine can benefit greatly from antibodies that deliver therapeutic and imaging agents to select organs and diseased tissues. Yet the development of complex and defined composite nanostructures remains a challenge that requires both designed stoichiometric assembly and superior in vivo testing ability. Here, we generate nanostructures called nanostreptabodies by controlled sequential assembly of biotin-engineered antibody fragments on a streptavidin scaffold with a defined capacity for additional biotinylated payloads such as other antibodies to create bispecific antibodies as well as organic and non-organic moieties. When injected intravenously, these novel and stable nanostructures exhibit exquisite targeting with tissue-specific imaging and delivery, including rapid transendothelial transport that enhances tissue penetration. This "tinkertoy construction" strategy provides a very flexible and efficient way to link targeting vectors with reporter and/or effector agents, thereby providing virtually endless combinations potentially useful for multipurpose molecular and functional imaging in vivo as well as therapies.

  8. Probing the Surface of Human Carbonic Anhydrase for Clues towards the Design of Isoform Specific Inhibitors

    PubMed Central

    Pinard, Melissa A.

    2015-01-01

    The alpha carbonic anhydrases (α-CAs) are a group of structurally related zinc metalloenzymes that catalyze the reversible hydration of CO2 to HCO3−. Humans have 15 different α-CAs with numerous physiological roles and expression patterns. Of these, 12 are catalytically active, and abnormal expression and activities are linked with various diseases, including glaucoma and cancer. Hence there is a need for CA isoform specific inhibitors to avoid off-target CA inhibition, but due to the high amino acid conservation of the active site and surrounding regions between each enzyme, this has proven difficult. However, residues towards the exit of the active site are variable and can be exploited to design isoform selective inhibitors. Here we discuss and characterize this region of “selective drug targetability” and how these observations can be utilized to develop isoform selective CA inhibitors. PMID:25811028

  9. Design of Miniaturized Double-Negative Material for Specific Absorption Rate Reduction in Human Head

    PubMed Central

    Faruque, Mohammad Rashed Iqbal; Islam, Mohammad Tariqul

    2014-01-01

    In this study, a double-negative triangular metamaterial (TMM) structure, which exhibits a resounding electric response at microwave frequency, was developed by etching two concentric triangular rings of conducting materials. A finite-difference time-domain method in conjunction with the lossy-Drude model was used in this study. Simulations were performed using the CST Microwave Studio. The specific absorption rate (SAR) reduction technique is discussed, and the effects of the position of attachment, the distance, and the size of the metamaterials on the SAR reduction are explored. The performance of the double-negative TMMs in cellular phones was also measured in the cheek and the tilted positions using the COMOSAR system. The TMMs achieved a 52.28% reduction for the 10 g SAR. These results provide a guideline to determine the triangular design of metamaterials with the maximum SAR reducing effect for a mobile phone. PMID:25350398

  10. A symmetrical, planar SOFC design for NASA's high specific power density requirements

    NASA Astrophysics Data System (ADS)

    Cable, Thomas L.; Sofie, Stephen W.

    Solid oxide fuel cell (SOFC) systems for aircraft applications require an order of magnitude increase in specific power density (1.0 kW kg -1) and long life. While significant research is underway to develop anode supported cells which operate at temperatures in the range of 650-800 °C, concerns about Cr-contamination from the metal interconnect may drive the operating temperature down further, to 750 °C and lower. Higher temperatures, 850-1000 °C, are more favorable in order to achieve specific power densities of 1.0 kW kg -1. Since metal interconnects are not practical at these high temperatures and can account for up to 75% of the weight of the stack, NASA is pursuing a design that uses a thin, LaCrO 3-based ceramic interconnect that incorporates gas channels into the electrodes. The bi-electrode supported cell (BSC) uses porous YSZ scaffolds, on either side of a 10-20 μm electrolyte. The porous support regions are fabricated with graded porosity using the freeze-tape casting process which can be tailored for fuel and air flow. Removing gas channels from the interconnect simplifies the stack design and allows the ceramic interconnect to be kept thin, on the order of 50-100 μm. The YSZ electrode scaffolds are infiltrated with active electrode materials following the high-temperature sintering step. The NASA-BSC is symmetrical and CTE matched, providing balanced stresses and favorable mechanical properties for vibration and thermal cycling.

  11. The Johnson Space Center Management Information Systems (JSCMIS). 1: Requirements Definition and Design Specifications for Versions 2.1 and 2.1.1. 2: Documented Test Scenario Environments. 3: Security Design and Specifications

    NASA Technical Reports Server (NTRS)

    1986-01-01

    The Johnson Space Center Management Information System (JSCMIS) is an interface to computer data bases at NASA Johnson which allows an authorized user to browse and retrieve information from a variety of sources with minimum effort. This issue gives requirements definition and design specifications for versions 2.1 and 2.1.1, along with documented test scenario environments, and security object design and specifications.

  12. Design of Internalizing PSMA-specific Glu-ureido-based Radiotherapeuticals.

    PubMed

    Wüstemann, Till; Bauder-Wüst, Ulrike; Schäfer, Martin; Eder, Matthias; Benesova, Martina; Leotta, Karin; Kratochwil, Clemens; Haberkorn, Uwe; Kopka, Klaus; Mier, Walter

    2016-01-01

    Despite the progress in diagnosis and treatment, prostate cancer (PCa) is one of the main causes for cancer-associated deaths among men. Recently, prostate-specific membrane antigen (PSMA) binding tracers have revolutionized the molecular imaging of this disease. The translation of these tracers into therapeutic applications is challenging because of high PSMA-associated kidney uptake. While both the tumor uptake and the uptake in the kidneys are PSMA-specific, the kidneys show a more rapid clearance than tumor lesions. Consequently, the potential of endoradiotherapeutic drugs targeting PSMA is highly dependent on a sustained retention in the tumor - ideally achieved by predominant internalization of the respective tracer. Previously, we were able to show that the pharmacokinetics of the tracers containing the Glu-urea-based binding motif can be further enhanced with a specifically designed linker. Here, we evaluate an eventual influence of the chelator moiety on the pharmacokinetics, including the tumor internalization. A series of tracers modified by different chelators were synthesized using solid phase chemistry. The conjugates were radiolabeled to evaluate the influence on the receptor binding affinity, the ligand-induced internalization and the biodistribution behavior. Competitive binding and internalization assays were performed on PSMA positive LNCaP cells and the biodistribution of the most promising compound was evaluated by positron emission tomography (PET) in LNCaP-tumor-bearing mice. Interestingly, conjugation of the different chelators did not cause significant differences: all compounds showed nanomolar binding affinities with only minor differences. PET imaging of the (68)Ga-labeled CHX-A''-DTPA conjugate revealed that the chelator moiety does not impair the specificity of tumor uptake when compared to the gold standard PSMA-617. However, strong differences of the internalization ratios caused by the chelator moiety were observed: differences in

  13. Design of Internalizing PSMA-specific Glu-ureido-based Radiotherapeuticals

    PubMed Central

    Wüstemann, Till; Bauder-Wüst, Ulrike; Schäfer, Martin; Eder, Matthias; Benesova, Martina; Leotta, Karin; Kratochwil, Clemens; Haberkorn, Uwe; Kopka, Klaus; Mier, Walter

    2016-01-01

    Despite the progress in diagnosis and treatment, prostate cancer (PCa) is one of the main causes for cancer-associated deaths among men. Recently, prostate-specific membrane antigen (PSMA) binding tracers have revolutionized the molecular imaging of this disease. The translation of these tracers into therapeutic applications is challenging because of high PSMA-associated kidney uptake. While both the tumor uptake and the uptake in the kidneys are PSMA-specific, the kidneys show a more rapid clearance than tumor lesions. Consequently, the potential of endoradiotherapeutic drugs targeting PSMA is highly dependent on a sustained retention in the tumor - ideally achieved by predominant internalization of the respective tracer. Previously, we were able to show that the pharmacokinetics of the tracers containing the Glu-urea-based binding motif can be further enhanced with a specifically designed linker. Here, we evaluate an eventual influence of the chelator moiety on the pharmacokinetics, including the tumor internalization. A series of tracers modified by different chelators were synthesized using solid phase chemistry. The conjugates were radiolabeled to evaluate the influence on the receptor binding affinity, the ligand-induced internalization and the biodistribution behavior. Competitive binding and internalization assays were performed on PSMA positive LNCaP cells and the biodistribution of the most promising compound was evaluated by positron emission tomography (PET) in LNCaP-tumor-bearing mice. Interestingly, conjugation of the different chelators did not cause significant differences: all compounds showed nanomolar binding affinities with only minor differences. PET imaging of the 68Ga-labeled CHX-A''-DTPA conjugate revealed that the chelator moiety does not impair the specificity of tumor uptake when compared to the gold standard PSMA-617. However, strong differences of the internalization ratios caused by the chelator moiety were observed: differences in

  14. Design of compact freeform lens for application specific Light-Emitting Diode packaging.

    PubMed

    Wang, Kai; Chen, Fei; Liu, Zongyuan; Luo, Xiaobing; Liu, Sheng

    2010-01-18

    Application specific LED packaging (ASLP) is an emerging technology for high performance LED lighting. We introduced a practical design method of compact freeform lens for extended sources used in ASLP. A new ASLP for road lighting was successfully obtained by integrating a polycarbonate compact freeform lens of small form factor with traditional LED packaging. Optical performance of the ASLP was investigated by both numerical simulation based on Monte Carlo ray tracing method and experiments. Results demonstrated that, comparing with traditional LED module integrated with secondary optics, the ASLP had advantages of much smaller size in volume (approximately 1/8), higher system lumen efficiency (approximately 8.1%), lower cost and more convenience for customers to design and assembly, enabling possible much wider applications of LED for general road lighting. Tolerance analyses were also conducted. Installation errors of horizontal and vertical deviations had more effects on the shape and uniformity of radiation pattern compared with rotational deviation. The tolerances of horizontal, vertical and rotational deviations of this lens were 0.11 mm, 0.14 mm and 2.4 degrees respectively, which were acceptable in engineering.

  15. Designing chitosan-tripolyphosphate microparticles with desired size for specific pharmaceutical or forensic applications.

    PubMed

    Hejjaji, Ezzeddin M A; Smith, Alan M; Morris, Gordon A

    2017-02-01

    Chitosan (CS) is a natural cationic polymer obtained by the partial N-deacetylation of chitin. Chitosan microparticles can be prepared by cross-linking with tripolyphosphate (TPP) via the ionic interaction between positively charged amino groups (CS) and negatively charged counter ions (TPP). This can be controlled by the charge density of CS and TPP, which depend on the pH and ionic strength of the solution. The purpose of this study is to investigate the combined effects of three independent variables (pH, ionic strength and CS:TPP ratio) on three important physico-chemical properties (viscosity, zeta potential and particle size) during the preparation of microparticles. CS:TPP microparticles were prepared using experimental design and equations were generated and used to predict relative viscosity, zeta potential and particle size under different conditions. This gives us the ability to design tuneable CS-TPP microparticles with desired size for specific pharmaceutical or forensic applications e.g. latent fingerprint visualisation.

  16. Design of a pseudo-physiological test bench specific to the development of biodegradable metallic biomaterials.

    PubMed

    Lévesque, Julie; Hermawan, Hendra; Dubé, Dominique; Mantovani, Diego

    2008-03-01

    Endovascular stents have proven effective in treating coronary and peripheral arterial occlusions. Since the first attempts, metals used to make these devices have been generally selected, and designed to be highly resistant to corrosion. Therefore, as almost the totality of metallic biomaterials, they are implanted on a long-term basis. However, complications associated with permanent stents, such as in-stent restenosis and thrombosis, have often been reported. In order to reduce those complications, it would be clinically useful to develop a new family of degradable stents. An interesting material for fabrication of degradable stents is based on magnesium, an essential element involved in human metabolism. Success in using magnesium alloys for the fabrication of endovascular devices is closely related to the properties of the selected alloy. In this context, a test bench was specifically designed to reproduce the physiological conditions to which stents are submitted when implanted in the coronary arteries. Then the test bench was validated using a magnesium-based alloy. Results showed that the corrosion rate and the corrosion mechanisms vary with the applied shear stress and that corrosion products strongly depend on the composition of the corrosive solution. This test bench will thus be useful in further investigations for the development of metallic alloys as degradable biomaterials.

  17. Acoustic Characteristics of Various Treatment Panel Designs Specific to HSCT Mixer-Ejector Application

    NASA Technical Reports Server (NTRS)

    Salikuddin, M.; Kinzie, K.; Vu, D. D.; Langenbrunner, L. E.; Szczepkowski, G. T.

    2006-01-01

    The development process of liner design methodology is described in several reports. The results of the initial effort of concept development, screening, laboratory testing of various liner concepts, and preliminary correlation (generic data) are presented in a report Acoustic Characteristics of Various Treatment Panel Designs for HSCT Ejector Liner Acoustic Technology Development Program. The second phase of laboratory test results of more practical concepts and their data correlations are presented in this report (product specific). In particular, this report contains normal incidence impedance measurements of several liner types in both a static rig and in a high temperature flow duct rig. The flow duct rig allows for temperatures up to 400 F with a grazing flow up to Mach 0.8. Measurements of impedance, DC flow resistance, and in the flow rig cases, impact of the liner on boundary layer profiles are documented. In addition to liner rig tests, a limited number of tests were made on liners installed in a mixer-Ejector nozzle to confirm the performance of the liner prediction in an installed configuration.

  18. Improving specific activity and thermostability of Escherichia coli phytase by structure-based rational design.

    PubMed

    Wu, Tzu-Hui; Chen, Chun-Chi; Cheng, Ya-Shan; Ko, Tzu-Ping; Lin, Cheng-Yen; Lai, Hui-Lin; Huang, Ting-Yung; Liu, Je-Ruei; Guo, Rey-Ting

    2014-04-10

    Escherichia coli phytase (EcAppA) which hydrolyzes phytate has been widely applied in the feed industry, but the need to improve the enzyme activity and thermostability remains. Here, we conduct rational design with two strategies to enhance the EcAppA performance. First, residues near the substrate binding pocket of EcAppA were modified according to the consensus sequence of two highly active Citrobacter phytases. One out of the eleven mutants, V89T, exhibited 17.5% increase in catalytic activity, which might be a result of stabilized protein folding. Second, the EcAppA glycosylation pattern was modified in accordance with the Citrobacter phytases. An N-glycosylation motif near the substrate binding site was disrupted to remove spatial hindrance for phytate entry and product departure. The de-glycosylated mutants showed 9.6% increase in specific activity. On the other hand, the EcAppA mutants that adopt N-glycosylation motifs from CbAppA showed improved thermostability that three mutants carrying single N-glycosylation motif exhibited 5.6-9.5% residual activity after treatment at 80°C (1.8% for wild type). Furthermore, the mutant carrying all three glycosylation motifs exhibited 27% residual activity. In conclusion, a successful rational design was performed to obtain several useful EcAppA mutants with better properties for further applications.

  19. Specific effects in microwave chemistry explored through reactor vessel design, theory, and spectroscopy.

    PubMed

    Ashley, Bridgett; Lovingood, Derek D; Chiu, Yu-Che; Gao, Hanwei; Owens, Jeffery; Strouse, Geoffrey F

    2015-11-07

    Microwave chemistry has revolutionized synthetic methodology for the preparation of organics, pharmaceuticals, materials, and peptides. The enhanced reaction rates commonly observed in a microwave have led to wide speculation about the function of molecular microwave absorption and whether the absorption leads to microwave specific effects and enhanced molecular heating. The comparison of theoretical modeling, reactor vessel design, and dielectric spectroscopy allows the nuance of the interaction to be directly understood. The study clearly shows an unaltered silicon carbide vessel allows measurable microwave penetration and therefore, molecular absorption of the microwave photons by the reactants within the reaction vessel cannot be ignored when discussing the role of molecular heating in enhanced molecular reactivity for microwave synthesis. The results of the study yield an improved microwave reactor vessel design that eliminates microwave leakage into the reaction volume by incorporating a noble metal surface layer onto a silicon carbide reaction vessel. The systematic study provides the necessary theory and measurements to better inform the arguments in the field.

  20. A proteomics search algorithm specifically designed for high-resolution tandem mass spectra.

    PubMed

    Wenger, Craig D; Coon, Joshua J

    2013-03-01

    The acquisition of high-resolution tandem mass spectra (MS/MS) is becoming more prevalent in proteomics, but most researchers employ peptide identification algorithms that were designed prior to this development. Here, we demonstrate new software, Morpheus, designed specifically for high-mass accuracy data, based on a simple score that is little more than the number of matching products. For a diverse collection of data sets from a variety of organisms (E. coli, yeast, human) acquired on a variety of instruments (quadrupole-time-of-flight, ion trap-orbitrap, and quadrupole-orbitrap) in different laboratories, Morpheus gives more spectrum, peptide, and protein identifications at a 1% false discovery rate (FDR) than Mascot, Open Mass Spectrometry Search Algorithm (OMSSA), and Sequest. Additionally, Morpheus is 1.5 to 4.6 times faster, depending on the data set, than the next fastest algorithm, OMSSA. Morpheus was developed in C# .NET and is available free and open source under a permissive license.

  1. Hydraulic design of a low-specific speed Francis runner for a hydraulic cooling tower

    NASA Astrophysics Data System (ADS)

    Ruan, H.; Luo, X. Q.; Liao, W. L.; Zhao, Y. P.

    2012-11-01

    The air blower in a cooling tower is normally driven by an electromotor, and the electric energy consumed by the electromotor is tremendous. The remaining energy at the outlet of the cooling cycle is considerable. This energy can be utilized to drive a hydraulic turbine and consequently to rotate the air blower. The purpose of this project is to recycle energy, lower energy consumption and reduce pollutant discharge. Firstly, a two-order polynomial is proposed to describe the blade setting angle distribution law along the meridional streamline in the streamline equation. The runner is designed by the point-to-point integration method with a specific blade setting angle distribution. Three different ultra-low-specificspeed Francis runners with different wrap angles are obtained in this method. Secondly, based on CFD numerical simulations, the effects of blade setting angle distribution on pressure coefficient distribution and relative efficiency have been analyzed. Finally, blade angles of inlet and outlet and control coefficients of blade setting angle distribution law are optimal variables, efficiency and minimum pressure are objective functions, adopting NSGA-II algorithm, a multi-objective optimization for ultra-low-specific speed Francis runner is carried out. The obtained results show that the optimal runner has higher efficiency and better cavitation performance.

  2. Design of Sequence-Specific DNA Binding Molecules for DNA Methyltransferase Inhibition

    PubMed Central

    2015-01-01

    The CpG dyad, an important genomic feature in DNA methylation and transcriptional regulation, is an attractive target for small molecules. To assess the utility of minor groove binding oligomers for CpG recognition, we screened a small library of hairpin pyrrole-imidazole polyamides targeting the sequence 5′-CGCG-3′ and assessed their sequence specificity using an unbiased next-generation sequencing assay. Our findings indicate that hairpin polyamide of sequence PyImβIm-γ-PyImβIm (1), previously identified as a high affinity 5′-CGCG-3′ binder, favors 5′-GCGC-3′ in an unanticipated reverse binding orientation. Replacement of one β alanine with Py to afford PyImPyIm-γ-PyImβIm (3) restores the preference for 5′-CGCG-3′ binding in a forward orientation. The minor groove binding hairpin 3 inhibits DNA methyltransferase activity in the major groove at its target site more effectively than 1, providing a molecular basis for design of sequence-specific antagonists of CpG methylation. PMID:24502234

  3. Modular concept for the design of application-specific integrated telemetric systems

    NASA Astrophysics Data System (ADS)

    Marschner, Claudia; Rehfuss, Sven; Peters, Dagmar; Bolte, Hilmar; Laur, Rainer

    2001-04-01

    Modern microsystem technology generates a great variety of very small sensors and actuators. With the use of capacitive measurement or moving principles these components have a very low power consumption and with some data preprocessing they can be used in passive telemetry systems, which need no integrated battery. Instead, they use two loosely coupled coils to realize the energy transfer. To create very small systems, e.g. for the implantation inside the human body, the use of an application specific integrated circuit for the telemetry will be necessary. In this paper a design strategy for the realization of implantable telemetric microsystems and a building set with different blocks for the creation of an application specific telemetry chip is presented. In addition to the necessary main building blocks like rectifiers, voltage regualtors and blocks for the uni- or bi-directional data transmission the set also includes some supplementary blocks like an auaotmatic resonance adjust. All blocks are realized in a standard CMOS process (a 0.7 um CMOS process with some analogue add-ons) and therfore very small and cheap systems can be created.

  4. Immunization with outer membrane vesicles displaying designer glycotopes yields class-switched, glycan-specific antibodies

    PubMed Central

    Valentine, Jenny L.; Chen, Linxiao; Perregaux, Emily C.; Weyant, Kevin B.; Rosenthal, Joseph A.; Heiss, Christian; Azadi, Parastoo; Fisher, Adam C.; Putnam, David; Moe, Gregory R.; Merritt, Judith H.; DeLisa, Matthew P.

    2016-01-01

    Summary The development of antibodies against specific glycan epitopes poses a significant challenge due to difficulties obtaining desired glycans at sufficient quantity and purity, and the fact that glycans are usually weakly immunogenic. To address this challenge, we leveraged the potent immunostimulatory activity of bacterial outer membrane vesicles (OMVs) to deliver designer glycan epitopes to the immune system. This approach involved heterologous expression of two clinically important glycans, namely polysialic acid (PSA) and Thomsen-Friedenreich antigen (T antigen) in hypervesiculating strains of non-pathogenic Escherichia coli. The resulting glycOMVs displayed structural mimics of PSA or T antigen on their surfaces, and induced high titers of glycan-specific IgG antibodies following immunization in mice. In the case of PSA glycOMVs, serum antibodies potently killed Neisseria meningitidis serogroup B (MenB), whose outer capsule is PSA, in a serum bactericidal assay. These findings demonstrate the potential of glycOMVs for inducing class-switched, humoral immune responses against glycan antigens. PMID:27341433

  5. Relaxing the coenzyme specificity of 1,3-propanediol oxidoreductase from Klebsiella pneumoniae by rational design.

    PubMed

    Ma, Chengwei; Zhang, Le; Dai, Jianying; Xiu, Zhilong

    2010-04-15

    1,3-Propanediol has wide applications for large volume markets, particularly in the polymer business. Microbial production of 1,3-propanediol has been considered as a competitor to the traditional petrochemical routes. However, the formation of 1,3-propanediol is limited by the amount of NADH supplied by the oxidative pathway of glycerol dismutation. Previous metabolic flux analysis revealed that relaxation of the coenzyme specificity of 1,3-propanediol oxidoreductase for both NADH and NADPH would increase the production of 1,3-propanediol as well as maintaining the NADH-NAD(+) circle. This work tried to accomplish such a relaxation by rational protein design. Overall binding free energy indicated that the electrostatic energy was the major force discriminating NADH from NADPH. Computational alanine-scanning mutagenesis of the active site residues illustrated that Asp41 was the key residue responsible for the coenzyme specificity. Compared with Asp41Ala, Asp41Gly could further weaken the repulsion between Asp41 and the phosphate group esterified to the 2'-hydroxyl group of the ribose at the adenine end of NADPH. Site-directed mutagenesis was conducted and the relaxation was successfully realized.

  6. Biomaterial design for specific cellular interactions: Role of surface functionalization and geometric features

    NASA Astrophysics Data System (ADS)

    Kolhar, Poornima

    The areas of drug delivery and tissue engineering have experienced extraordinary growth in recent years with the application of engineering principles and their potential to support and improve the field of medicine. The tremendous progress in nanotechnology and biotechnology has lead to this explosion of research and development in biomedical applications. Biomaterials can now be engineered at a nanoscale and their specific interactions with the biological tissues can be modulated. Various design parameters are being established and researched for design of drug-delivery carriers and scaffolds to be implanted into humans. Nanoparticles made from versatile biomaterial can deliver both small-molecule drugs and various classes of bio-macromolecules, such as proteins and oligonucleotides. Similarly in the field of tissue engineering, current approaches emphasize nanoscale control of cell behavior by mimicking the natural extracellular matrix (ECM) unlike, traditional scaffolds. Drug delivery and tissue engineering are closely connected fields and both of these applications require materials with exceptional physical, chemical, biological, and biomechanical properties to provide superior therapy. In the current study the surface functionalization and the geometric features of the biomaterials has been explored. In particular, a synthetic surface for culture of human embryonic stem cells has been developed, demonstrating the importance of surface functionalization in maintaining the pluripotency of hESCs. In the second study, the geometric features of the drug delivery carriers are investigated and the polymeric nanoneedles mediated cellular permeabilization and direct cytoplasmic delivery is reported. In the third study, the combined effect of surface functionalization and geometric modification of carriers for vascular targeting is enunciated. These studies illustrate how the biomaterials can be designed to achieve various cellular behaviors and control the

  7. Ligand tunnels in T. brucei and human CYP51: Insights for parasite-specific drug design

    PubMed Central

    Yu, Xiaofeng; Nandekar, Prajwal; Mustafa, Ghulam; Cojocaru, Vlad; Lepesheva, Galina I.; Wade, Rebecca C.

    2015-01-01

    Background Cytochrome P450 sterol 14α-demethylase (CYP51) is an essential enzyme for sterol biosynthesis and a target for anti-parasitic drug design. However, the design of parasite-specific drugs that inhibit parasitic CYP51 without severe side effects remains challenging. The active site of CYP51 is situated in the interior of the protein. Here, we characterize the potential ligand egress routes and mechanisms in Trypanosoma brucei and human CYP51 enzymes. Methods We performed Random Acceleration Molecular Dynamics simulations of the egress of four different ligands from the active site of models of soluble and membrane-bound T. brucei CYP51 and of soluble human CYP51. Results In the simulations, tunnel 2 f, which leads to the membrane, was found to be the predominant ligand egress tunnel for all the ligands studied. Tunnels S, 1 and W, which lead to the cytosol, were also used in T. brucei CYP51, whereas tunnel 1 was the only other tunnel used significantly in human CYP51. The common tunnels found previously in other CYPs were barely used. The ligand egress times were shorter for human than T. brucei CYP51, suggesting lower barriers to ligand passage. Two gating residues, F105 and M460, in T. brucei CYP51 that modulate the opening of tunnels 2 f and S were identified. Conclusions Although the main egress tunnel was the same, differences in the tunnel-lining residues, ligand passage and tunnel usage were found between T. brucei and human CYP51s. General Significance The results provide a basis for the design of selective anti-parasitic agents targeting the ligand tunnels. PMID:26493722

  8. 40 CFR 255.11 - Criteria for identifying agencies.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... quality management planning agencies designated under section 208 of the Federal Water Pollution Control... State water quality management agencies. (d) Planning objectives will influence agency selection... IDENTIFICATION OF REGIONS AND AGENCIES FOR SOLID WASTE MANAGEMENT Criteria for Identifying Regions and...

  9. 40 CFR 255.11 - Criteria for identifying agencies.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... quality management planning agencies designated under section 208 of the Federal Water Pollution Control... State water quality management agencies. (d) Planning objectives will influence agency selection... IDENTIFICATION OF REGIONS AND AGENCIES FOR SOLID WASTE MANAGEMENT Criteria for Identifying Regions and...

  10. 40 CFR 255.11 - Criteria for identifying agencies.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... quality management planning agencies designated under section 208 of the Federal Water Pollution Control... State water quality management agencies. (d) Planning objectives will influence agency selection... IDENTIFICATION OF REGIONS AND AGENCIES FOR SOLID WASTE MANAGEMENT Criteria for Identifying Regions and...

  11. 40 CFR 255.11 - Criteria for identifying agencies.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... quality management planning agencies designated under section 208 of the Federal Water Pollution Control... State water quality management agencies. (d) Planning objectives will influence agency selection... IDENTIFICATION OF REGIONS AND AGENCIES FOR SOLID WASTE MANAGEMENT Criteria for Identifying Regions and...

  12. 40 CFR 255.11 - Criteria for identifying agencies.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... quality management planning agencies designated under section 208 of the Federal Water Pollution Control... State water quality management agencies. (d) Planning objectives will influence agency selection... IDENTIFICATION OF REGIONS AND AGENCIES FOR SOLID WASTE MANAGEMENT Criteria for Identifying Regions and...

  13. Corporate Sector Practice Informs Online Workforce Training for Australian Government Agencies: Towards Effective Educational-Learning Systems Design

    ERIC Educational Resources Information Center

    McKay, Elspeth; Vilela, Cenie

    2011-01-01

    The purpose of this paper is to outline government online training practice. We searched individual research domains of the human-dimensions of Human Computer Interaction (HCI), information and communications technologies (ICT) and instructional design for evidence of either corporate sector or government training practices. We overlapped these…

  14. 12 CFR 1815.104 - Specific responsibilities of the designated Fund official.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... information and advice on the Fund's policies for protecting and enhancing the quality of the environment. ... Fund official. 1815.104 Section 1815.104 Banks and Banking COMMUNITY DEVELOPMENT FINANCIAL INSTITUTIONS... prepared by other agencies; (k) Acquire information and prepare appropriate reports on...

  15. Designing a C84 fullerene as a specific voltage-gated sodium channel blocker

    NASA Astrophysics Data System (ADS)

    Hilder, Tamsyn A.; Chung, Shin-Ho

    2013-07-01

    Fullerene derivatives demonstrate considerable potential for numerous biological applications, such as the effective inhibition of HIV protease. Recently, they were identified for their ability to indiscriminately block biological ion channels. A fullerene derivative which specifically blocks a particular ion channel could lead to a new set of drug leads for the treatment of various ion channel-related diseases. Here, we demonstrate their extraordinary potential by designing a fullerene which mimics some of the functions of μ-conotoxin, a peptide derived from cone snail venom which potently binds to the bacterial voltage-gated sodium channel (NavAb). We show, using molecular dynamics simulations, that the C84 fullerene with six lysine derivatives uniformly attached to its surface is selective to NavAb over a voltage-gated potassium channel (Kv1.3). The side chain of one of the lysine residues protrudes into the selectivity filter of the channel, while the methionine residues located just outside of the channel form hydrophobic contacts with the carbon atoms of the fullerene. The modified C84 fullerene strongly binds to the NavAb channel with an affinity of 46 nM but binds weakly to Kv1.3 with an affinity of 3 mM. This potent blocker of NavAb may serve as a structural template from which potent compounds can be designed for the targeting of mammalian Nav channels. There is a genuine need to target mammalian Nav channels as a form of treatment of various diseases which have been linked to their malfunction, such as epilepsy and chronic pain.

  16. Specifically Designed Constructed Wetlands: A Novel Treatment Approach for Scrubber Wastewater

    SciTech Connect

    John H. Rodgers Jr; James W. Castle; Chris Arrington: Derek Eggert; Meg Iannacone

    2005-09-01

    A pilot-scale wetland treatment system was specifically designed and constructed at Clemson University to evaluate removal of mercury, selenium, and other constituents from flue gas desulfurization (FGD) wastewater. Specific objectives of this research were: (1) to measure performance of a pilot-scale constructed wetland treatment system in terms of decreases in targeted constituents (Hg, Se and As) in the FGD wastewater from inflow to outflow; (2) to determine how the observed performance is achieved (both reactions and rates); and (3) to measure performance in terms of decreased bioavailability of these elements (i.e. toxicity of sediments in constructed wetlands and toxicity of outflow waters from the treatment system). Performance of the pilot-scale constructed wetland treatment systems was assessed using two criteria: anticipated NPDES permit levels and toxicity evaluations using two sentinel toxicity-testing organisms (Ceriodaphnia dubia and Pimephales promelas). These systems performed efficiently with varied inflow simulations of FGD wastewaters removing As, Hg, and Se concentrations below NPDES permit levels and reducing the toxicity of simulated FGD wastewater after treatment with the constructed wetland treatment systems. Sequential extraction procedures indicated that these elements (As, Hg, and Se) were bound to residual phases within sediments of these systems, which should limit their bioavailability to aquatic biota. Sediments collected from constructed wetland treatment systems were tested to observe toxicity to Hyalella azteca or Chironomus tetans. Complete survival (100%) was observed for H. azteca in all cells of the constructed wetland treatment system and C. tentans had an average of 91% survival over the three treatment cells containing sediments. Survival and growth of H. azteca and C. tentans did not differ significantly between sediments from the constructed wetland treatment system and controls. Since the sediments of the constructed

  17. Computational Biology Tools for Identifying Specific Ligand Binding Residues for Novel Agrochemical and Drug Design.

    PubMed

    Neshich, Izabella Agostinho Pena; Nishimura, Leticia; de Moraes, Fabio Rogerio; Salim, Jose Augusto; Villalta-Romero, Fabian; Borro, Luiz; Yano, Inacio Henrique; Mazoni, Ivan; Tasic, Ljubica; Jardine, Jose Gilberto; Neshich, Goran

    2015-01-01

    The term "agrochemicals" is used in its generic form to represent a spectrum of pesticides, such as insecticides, fungicides or bactericides. They contain active components designed for optimized pest management and control, therefore allowing for economically sound and labor efficient agricultural production. A "drug" on the other side is a term that is used for compounds designed for controlling human diseases. Although drugs are subjected to much more severe testing and regulation procedures before reaching the market, they might contain exactly the same active ingredient as certain agrochemicals, what is the case described in present work, showing how a small chemical compound might be used to control pathogenicity of Gram negative bacteria Xylella fastidiosa which devastates citrus plantations, as well as for control of, for example, meningitis in humans. It is also clear that so far the production of new agrochemicals is not benefiting as much from the in silico new chemical compound identification/discovery as pharmaceutical production. Rational drug design crucially depends on detailed knowledge of structural information about the receptor (target protein) and the ligand (drug/agrochemical). The interaction between the two molecules is the subject of analysis that aims to understand relationship between structure and function, mainly deciphering some fundamental elements of the nanoenvironment where the interaction occurs. In this work we will emphasize the role of understanding nanoenvironmental factors that guide recognition and interaction of target protein and its function modifier, an agrochemical or a drug. The repertoire of nanoenvironment descriptors is used for two selected and specific cases we have approached in order to offer a technological solution for some very important problems that needs special attention in agriculture: elimination of pathogenicity of a bacterium which is attacking citrus plants and formulation of a new fungicide. Finally

  18. Evaluation of a constructed wetland treatment system specifically designed to decrease bioavailable copper in a wastestream.

    PubMed

    Murray-Gulde, Cynthia L; Bearr, Jonathan; Rodgers, John H

    2005-05-01

    A specifically designed constructed wetland decreased copper concentrations in a wastestream to 22 microg Cu/L and eliminated associated toxicity. Metal toxicity is a function of both concentration and form. This research measured copper partitioning to ligands within the wetland and observed changes in copper form with regard to bioavailability. Average monthly copper concentrations in the constructed wetland treatment system ranged from 10 to 47 microg/L in the upstream (i.e., inflow to the constructed wetland prior to the retention basin) and from non-detection to 11 microg/L in the downstream (i.e., outflow to the receiving stream). On average, 78% total-recoverable, 85% acid-soluble, and 83% soluble copper were removed from inflow to outflow of this constructed wetland; however, total recoverable and acid-soluble copper measurements were not useful indicators of bioavailable copper. Survival of Ceriodaphnia dubia increased from an average of 2% survival in the inflow to 96% in the outflow and reproduction increased from an average of 8 to 24 neonates/female, respectively. Soluble copper is a more accurate predictor of the concentration at which effects were observed. Average ratio of acid volatile sulfides to simultaneously extractable metals was 1.4: 0.06 micromol/g indicating sufficient sulfides to sequester available metals in the system.

  19. Risk based approach for design and optimization of stomach specific delivery of rifampicin.

    PubMed

    Vora, Chintan; Patadia, Riddhish; Mittal, Karan; Mashru, Rajashree

    2013-10-15

    The research envisaged focuses on risk management approach for better recognizing the risks, ways to mitigate them and propose a control strategy for the development of rifampicin gastroretentive tablets. Risk assessment using failure mode and effects analysis (FMEA) was done to depict the effects of specific failure modes related to respective formulation/process variable. A Box-Behnken design was used to investigate the effect of amount of sodium bicarbonate (X1), pore former HPMC (X2) and glyceryl behenate (X3) on percent drug release at 1st hour (Q1), 4th hour (Q4), 8th hour (Q8) and floating lag time (min). Main effects and interaction plots were generated to study effects of variables. Selection of the optimized formulation was done using desirability function and overlay contour plots. The optimized formulation exhibited Q1 of 20.9%, Q4 of 59.1%, Q8 of 94.8% and floating lag time of 4.0 min. Akaike information criteria and Model selection criteria revealed that the model was best described by Korsmeyer-Peppas power law. The residual plots demonstrated no existence of non-normality, skewness or outliers. The composite desirability for optimized formulation computed using equations and software were 0.84 and 0.86 respectively. FTIR, DSC and PXRD studies ruled out drug polymer interaction due to thermal treatment.

  20. Mechanism of action and specificity of antimicrobial peptides designed based on buforin IIb.

    PubMed

    Jang, Su A; Kim, Hyun; Lee, Ju Young; Shin, Ju Ri; Kim, Da Jung; Cho, Ju Hyun; Kim, Sun Chang

    2012-04-01

    Buforin IIb-a synthetic analog of buforin II that contains a proline hinge between the two α-helices and a model α-helical sequence at the C-terminus (3× RLLR)-is a potent cell-penetrating antimicrobial peptide. To develop novel antimicrobial peptides with enhanced activities and specificity/therapeutic index, we designed several analogs (Buf III analogs) by substitutions of amino acids in the proline hinge region and two α-helices of buforin IIb, and examined their antimicrobial activity and mechanism of action. The substitution of hydrophobic residues ([F(6)] and [V(8)]) in the proline hinge region with other hydrophobic residues ([W(6)] and [I(8)]) did not affect antimicrobial activity, while the substitution of the first four amino acids RAGL with a model α-helical sequence increased the antimicrobial activity up to 2-fold. Like buforin IIb, Buf III analogs penetrated the bacterial cell membranes without significantly permeabilizing them and were accumulated inside Escherichia coli. Buf III analogs were shown to bind DNA in vitro and the DNA binding affinity of the peptides correlated linearly with their antimicrobial potency. Among the Buf III analogs, the therapeutic index of Buf IIIb and IIIc (RVVRQWPIG[RVVR](3) and KLLKQWPIG[KLLK](3), respectively) were improved 7-fold compared to that of buforin IIb. These results indicate that Buf III analogs appear to be promising candidates for future development as novel antimicrobial agents.

  1. Site-specific integration and tailoring of cassette design for sustainable gene transfer.

    PubMed

    Lombardo, Angelo; Cesana, Daniela; Genovese, Pietro; Di Stefano, Bruno; Provasi, Elena; Colombo, Daniele F; Neri, Margherita; Magnani, Zulma; Cantore, Alessio; Lo Riso, Pietro; Damo, Martina; Pello, Oscar M; Holmes, Michael C; Gregory, Philip D; Gritti, Angela; Broccoli, Vania; Bonini, Chiara; Naldini, Luigi

    2011-08-21

    Integrative gene transfer methods are limited by variable transgene expression and by the consequences of random insertional mutagenesis that confound interpretation in gene-function studies and may cause adverse events in gene therapy. Site-specific integration may overcome these hurdles. Toward this goal, we studied the transcriptional and epigenetic impact of different transgene expression cassettes, targeted by engineered zinc-finger nucleases to the CCR5 and AAVS1 genomic loci of human cells. Analyses performed before and after integration defined features of the locus and cassette design that together allow robust transgene expression without detectable transcriptional perturbation of the targeted locus and its flanking genes in many cell types, including primary human lymphocytes. We thus provide a framework for sustainable gene transfer in AAVS1 that can be used for dependable genetic manipulation, neutral marking of the cell and improved safety of therapeutic applications, and demonstrate its feasibility by rapidly generating human lymphocytes and stem cells carrying targeted and benign transgene insertions.

  2. Design of Protein Multi-specificity Using an Independent Sequence Search Reduces the Barrier to Low Energy Sequences

    PubMed Central

    Sevy, Alexander M.; Jacobs, Tim M.; Crowe, James E.; Meiler, Jens

    2015-01-01

    Computational protein design has found great success in engineering proteins for thermodynamic stability, binding specificity, or enzymatic activity in a ‘single state’ design (SSD) paradigm. Multi-specificity design (MSD), on the other hand, involves considering the stability of multiple protein states simultaneously. We have developed a novel MSD algorithm, which we refer to as REstrained CONvergence in multi-specificity design (RECON). The algorithm allows each state to adopt its own sequence throughout the design process rather than enforcing a single sequence on all states. Convergence to a single sequence is encouraged through an incrementally increasing convergence restraint for corresponding positions. Compared to MSD algorithms that enforce (constrain) an identical sequence on all states the energy landscape is simplified, which accelerates the search drastically. As a result, RECON can readily be used in simulations with a flexible protein backbone. We have benchmarked RECON on two design tasks. First, we designed antibodies derived from a common germline gene against their diverse targets to assess recovery of the germline, polyspecific sequence. Second, we design “promiscuous”, polyspecific proteins against all binding partners and measure recovery of the native sequence. We show that RECON is able to efficiently recover native-like, biologically relevant sequences in this diverse set of protein complexes. PMID:26147100

  3. Design of Protein Multi-specificity Using an Independent Sequence Search Reduces the Barrier to Low Energy Sequences.

    PubMed

    Sevy, Alexander M; Jacobs, Tim M; Crowe, James E; Meiler, Jens

    2015-07-01

    Computational protein design has found great success in engineering proteins for thermodynamic stability, binding specificity, or enzymatic activity in a 'single state' design (SSD) paradigm. Multi-specificity design (MSD), on the other hand, involves considering the stability of multiple protein states simultaneously. We have developed a novel MSD algorithm, which we refer to as REstrained CONvergence in multi-specificity design (RECON). The algorithm allows each state to adopt its own sequence throughout the design process rather than enforcing a single sequence on all states. Convergence to a single sequence is encouraged through an incrementally increasing convergence restraint for corresponding positions. Compared to MSD algorithms that enforce (constrain) an identical sequence on all states the energy landscape is simplified, which accelerates the search drastically. As a result, RECON can readily be used in simulations with a flexible protein backbone. We have benchmarked RECON on two design tasks. First, we designed antibodies derived from a common germline gene against their diverse targets to assess recovery of the germline, polyspecific sequence. Second, we design "promiscuous", polyspecific proteins against all binding partners and measure recovery of the native sequence. We show that RECON is able to efficiently recover native-like, biologically relevant sequences in this diverse set of protein complexes.

  4. Course Design and Delivery Specifications as a Tool for Ensuring Quality in an Online Training Program

    ERIC Educational Resources Information Center

    Docq, Françoise

    2015-01-01

    This case discusses the design, implementation, and regulation of a hybrid training program (60 credits over two years) organised by three business schools in Europe, and stretching over a five-year period. Following an incremental design process, the design team faced multiple challenges, from finding the added value of hybridization to choosing…

  5. SSME Alternate Turbopump Development Program: Design verification specification for high-pressure fuel turbopump

    NASA Technical Reports Server (NTRS)

    1989-01-01

    The design and verification requirements are defined which are appropriate to hardware at the detail, subassembly, component, and engine levels and to correlate these requirements to the development demonstrations which provides verification that design objectives are achieved. The high pressure fuel turbopump requirements verification matrix provides correlation between design requirements and the tests required to verify that the requirement have been met.

  6. Structure based re-design of the binding specificity of anti-apoptotic Bcl-xL

    PubMed Central

    Chen, T. Scott; Palacios, Hector; Keating, Amy E.

    2012-01-01

    Many native proteins are multi-specific and interact with numerous partners, which can confound analysis of their functions. Protein design provides a potential route to generating synthetic variants of native proteins with more selective binding profiles. Re-designed proteins could be used as research tools, diagnostics or therapeutics. In this work, we used a library screening approach to re-engineer the multi-specific anti-apoptotic protein Bcl-xL to remove its interactions with many of its binding partners, making it a high affinity and selective binder of the BH3 region of pro-apoptotic protein Bad. To overcome the enormity of the potential Bcl-xL sequence space, we developed and applied a computational/experimental framework that used protein structure information to generate focused combinatorial libraries. Sequence features were identified using structure-based modeling, and an optimization algorithm based on integer programming was used to select degenerate codons that maximally covered these features. A constraint on library size was used to ensure thorough sampling. Using yeast surface display to screen a designed library of Bcl-xL variants, we successfully identified a protein with ~1,000-fold improvement in binding specificity for the BH3 region of Bad over the BH3 region of Bim. Although negative design was targeted only against the BH3 region of Bim, the best re-designed protein was globally specific against binding to 10 other peptides corresponding to native BH3 motifs. Our design framework demonstrates an efficient route to highly specific protein binders and may readily be adapted for application to other design problems. PMID:23154169

  7. UMTRA Project remedial action planning and disposal cell design to comply with the proposed EPA (Environmental Protection Agency) standards (40 CFR Part 192)

    SciTech Connect

    Not Available

    1989-01-01

    The Uranium Mill Tailings Remedial Action (UMTRA) Project involves stabilizing 24 inactive uranium mill tailings piles in 10 states. Remedial work must meet standards established by the US Environmental Protection Agency (EPA). Remedial action must be designed and constructed to prevent dispersion of the tailings and other contaminated materials, and must prevent the inadvertent use of the tailings by man. This report is prepared primarily for distribution to parties involved in the UMTRA Project, including the US Nuclear Regulatory Commission (NRC), and states and tribes. It is intended to record the work done by the DOE since publication of the proposed EPA groundwater protection standards, and to show how the DOE has attempted to respond and react in a positive way to the new requirements that result from the proposed standards. This report discusses the groundwater compliance strategies now being defined and implemented by the DOE, and details the changes in disposal cell designs that result from studies to evaluate ways to facilitate compliance with the proposed EPA groundwater protection standards. This report also serves to record the technical advances, planning, and progress made on the UMTRA Project since the appearance of the proposed EPA groundwater protection standards. The report serves to establish, document, and disseminate technical approaches and engineering and groundwater information to people who may be interested or involved in similar or related projects. 24 refs., 27 figs., 8 tabs.

  8. Optical design of the single-detector planetary stereo camera for the BepiColombo European Space Agency mission to Mercury.

    PubMed

    Da Deppo, Vania; Naletto, Giampiero; Cremonese, Gabriele; Calamai, Luciano

    2010-05-20

    We present the catadioptric optical design solution for the stereo channel of the imaging system SIMBIOSYS for the BepiColombo European Space Agency mission to Mercury. The main scientific objectives of the instrument are the three-dimensional global mapping of the entire surface of Mercury in the panchromatic band and imaging of selected areas in four broad colored bands; both tasks have to be accomplished with a scale factor of 50?m per pixel at periherm. The system consists of an original compact layout in which the two stereo subchannels share a common detector; also, the optical components are common to the two subchannels, with the exception of the first element, which is a rhomboid prism. The field of view of each subchannel is about 5 degrees x5 degrees with a scale factor of 23 arcsec/pixel. The ray-tracing simulation of the system shows that the design guarantees optimal aberration balancing over the entire field of view and the entire wavelength range covered by the instrument, with ensquared energy of the order of 80% in one pixel.

  9. A minority research and education information service: Design, develop, pilot test, and implement on-line access for historically black colleges and universities and government agencies

    SciTech Connect

    Rodman, J.A.

    1992-01-01

    This Annual Status Report describes the design, development and implementation of the Minority On-Line Information Service (MOLIS) project by Federal Information Exchange, Inc. for the period of April 1, 1991 to March 31, 1992. Summary information detailing developments prior to this reporting period will also be included to establish a comprehensive perspective of the project. The goal of the MOLIS project, was to develop, design, pilot test on-line access to current information on minority colleges and universities and federal minority opportunities. Federal Information Exchange, Inc. (FIE), a diversified information services company recognized by researchers and educators as a leader in the field of information delivery services, was awarded a 5 year small business research grant to develop and implement MOLIS. Since April 29, 1991, the inauguration of its on-line service, MOLIS has provided current information on 138 Black and Hispanic colleges and universities -- including faculty and student profiles, financial data, research centers and equipment information, pre-college and education programs, emerging capabilities, enrollment data, administrative personnel data, and current events -- as well as minority opportunities from 8 participating federal agencies.

  10. Feasibility of developing a portable driver performance data acquisition system for human factors research: Design specifications. Volume 3

    SciTech Connect

    Carter, R.J.; Barickman, F.S.

    1998-01-01

    A two-phase, multi-year research program entitled ``development of a portable driver performance data acquisition system for human factors research was recently completed. The primary objective of the project was to develop a portable data acquisition system for crash avoidance research (DASCAR) that will allow driver performance data to be collected using a large variety of vehicle types and that would be capable of being installed on a given vehicle type within a relatively short-time frame. During Phase 1 a feasibility study for designing and fabricating DASCAR was conducted. In phase 2 of the research DASCAR was actually developed and validated. This technical memorandum documents the results from the feasibility study. It is subdivided into three volumes. Volume one addresses the last five items in the phase 1 research and the first issue in the second phase of the project. Volume 2 presents the related appendices. Volume three (this report) displays the design specifications developed for DASCAR during the ``develop design requirements and specifications for a portable driver performance data acquisition system`` task. Design specifications were assembled for each DASCAR element. The specifications were prepared in sufficient detail to allow a third party to use them to design, develop, procure, and subsequently construct the data acquisition system. This report also covers the background to the program.

  11. 40 CFR 55.15 - Specific designation of corresponding onshore areas.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... corresponding onshore areas. (a) California. (1) The South Coast Air Quality Management District is designated... the following OCS facilities: Habitat, Hacienda, Harmony, Harvest, Heather, Henry, Heritage,...

  12. Bioinspired Design and Oriented Synthesis of Immunogenic Site-Specifically Penicilloylated Peptides.

    PubMed

    Scornet, Noémie; Delarue-Cochin, Sandrine; Azoury, Marie Eliane; Le Mignon, Maxime; Chemelle, Julie-Anne; Nony, Emmanuel; Maillère, Bernard; Terreux, Raphaël; Pallardy, Marc; Joseph, Delphine

    2016-11-16

    β-Lactam antibiotics allergy is recognized as a public health concern. By covalently binding to serum proteins, penicillins are known to form immunogenic complexes. The latter are recognized and digested by antigen-presenting cells into drug-hapten peptides leading to the immunization of treated persons and IgE-mediated hypersensitivity reactions encompassing anaphylaxis. If type I allergic reactions to drugs are often unpredictable, they are known to be dependent on CD4(+) T-cells. This fundamental study revisits the chemical basis of the benzylpenicillin (BP) allergy with the aim of identifying immunologically relevant biomimetic benzylpenicilloylated peptides through the analysis of BP-conjugated human serum albumin (BP-HSA) profile and the evaluation of the naı̈ve CD4(+) T-cell responses to candidate BP-HSA-derived peptides. The chemical structures of BP-HSA bioconjugates synthesized in vitro at both physiological and basic pH were investigated by mass spectrometry. From the ten most representative lysine residues grafted by BP-hapten, HSA-bioinspired 15-mer peptide sequences were designed and the potential T-cell epitope profile of each peptide was predicted using two complementary in silico approaches, i.e., HLA class II binding prediction tools from the Immune Epitope Database and Analysis Resource (IEDB) and computational alanine scanning mutagenesis. Twelve structurally diversified benzylpenicilloylated peptides (BP-Ps) were selected and synthesized with the aid of a flexible synthesis pathway using an original benzylpenicilloylated lysine monomer as common precursor. In order to corroborate their predicted "epitope" profile, the naı̈ve CD4(+) T-cell response specific to BP was evaluated through a coculture approach. To our knowledge, this study showed for the first time the ability of bioinspired peptides structurally stemming from BP-HSA to be recognized by naı̈ve CD4(+) T-cells thus identifying a pre-existing T-cell repertoire for penicillin

  13. Design of a specific activator for skeletal muscle sodium channels uncovers channel architecture.

    PubMed

    Cohen, Lior; Ilan, Nitza; Gur, Maya; Stühmer, Walter; Gordon, Dalia; Gurevitz, Michael

    2007-10-05

    Gating modifiers of voltage-gated sodium channels (Na(v)s) are important tools in neuroscience research and may have therapeutic potential in medicinal disorders. Analysis of the bioactive surface of the scorpion beta-toxin Css4 (from Centruroides suffusus suffusus) toward rat brain (rNa(v)1.2a) and skeletal muscle (rNa(v)1.4) channels using binding studies revealed commonality but also substantial differences, which were used to design a specific activator, Css4(F14A/E15A/E28R), of rNa(v)1.4 expressed in Xenopus oocytes. The therapeutic potential of Css4(F14A/E15A/E28R) was tested using an rNa(v)1.4 mutant carrying the same mutation present in the genetic disorder hypokalemic periodic paralysis. The activator restored the impaired gating properties of the mutant channel expressed in oocytes, thus offering a tentative new means for treatment of neuromuscular disorders with reduced muscle excitability. Mutant double cycle analysis employing toxin residues involved in the construction of Css4(F14A/E15A/E28R) and residues whose equivalents in the rat brain channel rNa(v)1.2a were shown to affect Css4 binding revealed significant coupling energy (>1.3 kcal/mol) between F14A and E592A at Domain-2/voltage sensor segments 1-2 (D2/S1-S2), R27Q and E1251N at D3/SS2-S6, and E28R with both E650A at D2/S3-S4 and E1251N at D3/SS2-S6. These results show that despite the differences in interactions with the rat brain and skeletal muscle Na(v)s, Css4 recognizes a similar region on both channel subtypes. Moreover, our data indicate that the S3-S4 loop of the voltage sensor module in Domain-2 is in very close proximity to the SS2-S6 segment of the pore module of Domain-3 in rNa(v)1.4. This is the first experimental evidence that the inter-domain spatial organization of mammalian Na(v)s resembles that of voltage-gated potassium channels.

  14. A Fiber-Optic Probe Design for Combustion Chamber Flame Detection Applications-Design Criteria, Performance Specifications, and Fabrication Technique

    NASA Technical Reports Server (NTRS)

    Borg, Stephen E.; Harper, Samuel E.

    2001-01-01

    This paper documents the design and development of the fiber-optic probes utilized in the flame detection systems used in NASA Langley Research Center's 8-Foot High Temperature Tunnel (8-ft HTT). Two independent flame detection systems are utilized to monitor the presence and stability of the main-burner and pilot-level flames during facility operation. Due to the harsh environment within the combustor, the successful development of a rugged and efficient fiber-optic probe was a critical milestone in the development of these flame detection systems. The final optical probe design for the two flame detection systems resulted from research that was conducted in Langley's 7-in High Temperature Pilot Tunnel (7-in HTT). A detailed description of the manufacturing process behind the optical probes used in the 8-ft HTT is provided in Appendix A of this report.

  15. Design-Build-Write: Increasing the Impact of English for Specific Purposes Learning and Teaching in Aeronautical Engineering Education through Multiple Intelligences Task Design

    ERIC Educational Resources Information Center

    Tatzl, Dietmar

    2011-01-01

    This article presents an English for Specific Purposes (ESP) task developed for teaching aeronautical engineering students. The task Design-Build-Write rests on the assumption that engineering students are skilled at mathematical reasoning, problem solving, drawing and constructing. In Gardner's 1983 Multiple Intelligences (MI) theory, these…

  16. Design and efficient production of bovine enterokinase light chain with higher specificity in E. coli.

    PubMed

    Chun, Haarin; Joo, Keehyoung; Lee, Jooyoung; Shin, Hang-Cheol

    2011-06-01

    Enterokinase light chain (EKL) is a serine protease that recognizes Asp-Asp-Asp-Asp-Lys (D(4)K) sequence and cleaves the C-terminal peptide bond of the lysine residue. The utility of EKL as a site-specific cleavage enzyme is hampered by sporadic cleavage at other sites than the canonical D(4)K recognition sequence. In order to produce more site-specific EKL, we have generated several EKL mutants in E. coli with substitutions at Tyr174 and Lys99 using PDI (protein disulfide isomerase) fusion system. Substitution of Tyr174 by basic residues confers higher specificity on EKL. The production of EKL with higher specificity could widen the utility of EKL as a site-specific cleavage enzyme to produce various recombinant proteins with therapeutic or industrial values.

  17. A RFID specific participatory design approach to support design and implementation of real-time location systems in the operating room.

    PubMed

    Guédon, A C P; Wauben, L S G L; de Korne, D F; Overvelde, M; Dankelman, J; van den Dobbelsteen, J J

    2015-01-01

    Information technology, such as real-time location (RTL) systems using Radio Frequency IDentification (RFID) may contribute to overcome patient safety issues and high costs in healthcare. The aim of this work is to study if a RFID specific Participatory Design (PD) approach supports the design and the implementation of RTL systems in the Operating Room (OR). A RFID specific PD approach was used to design and implement two RFID based modules. The Device Module monitors the safety status of OR devices and the Patient Module tracks the patients' locations during their hospital stay. The PD principles 'multidisciplinary team', 'participation users (active involvement)' and 'early adopters' were used to include users from the RFID company, the university and the hospital. The design and implementation process consisted of two 'structured cycles' ('iterations'). The effectiveness of this approach was assessed by the acceptance in terms of level of use, continuity of the project and purchase. The Device Module included eight strategic and twelve tactical actions and the Patient Module included six strategic and twelve tactical actions. Both modules are now used on a daily basis and are purchased by the hospitals for continued use. The RFID specific PD approach was effective in guiding and supporting the design and implementation process of RFID technology in the OR. The multidisciplinary teams and their active participation provided insights in the social and the organizational context of the hospitals making it possible to better fit the technology to the hospitals' (future) needs.

  18. Estimating the designated use attainment decision error rates of US Environmental Protection Agency's proposed numeric total phosphorus criteria for Florida, USA, colored lakes.

    PubMed

    McLaughlin, Douglas B

    2012-01-01

    The utility of numeric nutrient criteria established for certain surface waters is likely to be affected by the uncertainty that exists in the presence of a causal link between nutrient stressor variables and designated use-related biological responses in those waters. This uncertainty can be difficult to characterize, interpret, and communicate to a broad audience of environmental stakeholders. The US Environmental Protection Agency (USEPA) has developed a systematic planning process to support a variety of environmental decisions, but this process is not generally applied to the development of national or state-level numeric nutrient criteria. This article describes a method for implementing such an approach and uses it to evaluate the numeric total P criteria recently proposed by USEPA for colored lakes in Florida, USA. An empirical, log-linear relationship between geometric mean concentrations of total P (a potential stressor variable) and chlorophyll a (a nutrient-related response variable) in these lakes-that is assumed to be causal in nature-forms the basis for the analysis. The use of the geometric mean total P concentration of a lake to correctly indicate designated use status, defined in terms of a 20 µg/L geometric mean chlorophyll a threshold, is evaluated. Rates of decision errors analogous to the Type I and Type II error rates familiar in hypothesis testing, and a 3rd error rate, E(ni) , referred to as the nutrient criterion-based impairment error rate, are estimated. The results show that USEPA's proposed "baseline" and "modified" nutrient criteria approach, in which data on both total P and chlorophyll a may be considered in establishing numeric nutrient criteria for a given lake within a specified range, provides a means for balancing and minimizing designated use attainment decision errors.

  19. Optimal design of a 3-leg 6-DOF parallel manipulator for a specific workspace

    NASA Astrophysics Data System (ADS)

    Fu, Jianxun; Gao, Feng

    2016-07-01

    Researchers seldom study optimum design of a six-degree-of-freedom(DOF) parallel manipulator with three legs based upon the given workspace. An optimal design method of a novel three-leg six-DOF parallel manipulator(TLPM) is presented. The mechanical structure of this robot is introduced, with this structure the kinematic constrain equations is decoupled. Analytical solutions of the forward kinematics are worked out, one configuration of this robot, including position and orientation of the end-effector are graphically displayed. Then, on the basis of several extreme positions of the kinematic performances, the task workspace is given. An algorithm of optimal designing is introduced to find the smallest dimensional parameters of the proposed robot. Examples illustrate the design results, and a design stability index is introduced, which ensures that the robot remains a safe distance from the boundary of sits actual workspace. Finally, one prototype of the robot is developed based on this method. This method can easily find appropriate kinematic parameters that can size a robot having the smallest workspace enclosing a predefined task workspace. It improves the design efficiency, ensures that the robot has a small mechanical size possesses a large given workspace volume, and meets the lightweight design requirements.

  20. Specifications for and preliminary design of a plant growth chamber for orbital experimental experiments

    NASA Technical Reports Server (NTRS)

    Sweet, H. C.; Simmonds, R. C.

    1976-01-01

    It was proposed that plant experiments be performed on board the space shuttle. To permit the proper execution of most tests, the craft must contain a plant growth chamber which is adequately designed to control those environmental factors which can induce changes in a plant's physiology and morphology. The various needs of, and environmental factors affecting, plants are identified. The permissilbe design, construction and performance limits for a plant-growth chamber are set, and tentative designs were prepared for units which are compatible with both the botanical requirements and the constraints imposed by the space shuttle.

  1. Sentence Comprehension in Specific Language Impairment: A Task Designed to Distinguish between Cognitive Capacity and Syntactic Complexity

    ERIC Educational Resources Information Center

    Leonard, Laurence B.; Deevy, Patricia; Fey, Marc E.; Bredin-Oja, Shelley L.

    2013-01-01

    Purpose: This study examined sentence comprehension in children with specific language impairment (SLI) in a manner designed to separate the contribution of cognitive capacity from the effects of syntactic structure. Method: Nineteen children with SLI, 19 typically developing children matched for age (TD-A), and 19 younger typically developing…

  2. Promoting Health-Related Fitness for Elementary Students with Intellectual Disabilities through a Specifically Designed Activity Program

    ERIC Educational Resources Information Center

    Davis, Kathryn; Zhang, Guili; Hodson, Patricia

    2011-01-01

    The Motivate, Adapt, and Play Program was specifically designed as an adapted physical activity (PA) program for students with intellectual disabilities (ID) to meet required school PA policies to combat childhood obesity. The policies commonly require a minimum of 30 min of PA per school day. A study was undertaken to test the efficacy of the…

  3. Putative and unique gene sequence utilization for the design of species specific probes as modeled by Lactobacillus plantarum

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The concept of utilizing putative and unique gene sequences for the design of species specific probes was tested. The abundance profile of assigned functions within the Lactobacillus plantarum genome was used for the identification of the putative and unique gene sequence, csh. The targeted gene (cs...

  4. Computational design of an epitope-specific Keap1 binding antibody using hotspot residues grafting and CDR loop swapping

    PubMed Central

    Liu, Xiaofeng; Taylor, Richard D.; Griffin, Laura; Coker, Shu-Fen; Adams, Ralph; Ceska, Tom; Shi, Jiye; Lawson, Alastair D. G.; Baker, Terry

    2017-01-01

    Therapeutic and diagnostic applications of monoclonal antibodies often require careful selection of binders that recognize specific epitopes on the target molecule to exert a desired modulation of biological function. Here we present a proof-of-concept application for the rational design of an epitope-specific antibody binding with the target protein Keap1, by grafting pre-defined structural interaction patterns from the native binding partner protein, Nrf2, onto geometrically matched positions of a set of antibody scaffolds. The designed antibodies bind to Keap1 and block the Keap1-Nrf2 interaction in an epitope-specific way. One resulting antibody is further optimised to achieve low-nanomolar binding affinity by in silico redesign of the CDRH3 sequences. An X-ray co-crystal structure of one resulting design reveals that the actual binding orientation and interface with Keap1 is very close to the design model, despite an unexpected CDRH3 tilt and VH/VL interface deviation, which indicates that the modelling precision may be improved by taking into account simultaneous CDR loops conformation and VH/VL orientation optimisation upon antibody sequence change. Our study confirms that, given a pre-existing crystal structure of the target protein-protein interaction, hotspots grafting with CDR loop swapping is an attractive route to the rational design of an antibody targeting a pre-selected epitope. PMID:28128368

  5. Design and Implementation of an On-Chip Patient-Specific Closed-Loop Seizure Onset and Termination Detection System.

    PubMed

    Zhang, Chen; Bin Altaf, Muhammad Awais; Yoo, Jerald

    2016-07-01

    This paper presents the design of an area- and energy-efficient closed-loop machine learning-based patient-specific seizure onset and termination detection algorithm, and its on-chip hardware implementation. Application- and scenario-based tradeoffs are compared and reviewed for seizure detection and suppression algorithm and system which comprises electroencephalography (EEG) data acquisition, feature extraction, classification, and stimulation. Support vector machine achieves a good tradeoff among power, area, patient specificity, latency, and classification accuracy for long-term monitoring of patients with limited training seizure patterns. Design challenges of EEG data acquisition on a multichannel wearable environment for a patch-type sensor are also discussed in detail. Dual-detector architecture incorporates two area-efficient linear support vector machine classifiers along with a weight-and-average algorithm to target high sensitivity and good specificity at once. On-chip implementation issues for a patient-specific transcranial electrical stimulation are also discussed. The system design is verified using CHB-MIT EEG database [1] with a comprehensive measurement criteria which achieves high sensitivity and specificity of 95.1% and 96.2%, respectively, with a small latency of 1 s. It also achieves seizure onset and termination detection delay of 2.98 and 3.82 s, respectively, with seizure length estimation error of 4.07 s.

  6. Mechanical design and performance specifications of anthropomorphic prosthetic hands: a review.

    PubMed

    Belter, Joseph T; Segil, Jacob L; Dollar, Aaron M; Weir, Richard F

    2013-01-01

    In this article, we set forth a detailed analysis of the mechanical characteristics of anthropomorphic prosthetic hands. We report on an empirical study concerning the performance of several commercially available myoelectric prosthetic hands, including the Vincent, iLimb, iLimb Pulse, Bebionic, Bebionic v2, and Michelangelo hands. We investigated the finger design and kinematics, mechanical joint coupling, and actuation methods of these commercial prosthetic hands. The empirical findings are supplemented with a compilation of published data on both commercial and prototype research prosthetic hands. We discuss numerous mechanical design parameters by referencing examples in the literature. Crucial design trade-offs are highlighted, including number of actuators and hand complexity, hand weight, and grasp force. Finally, we offer a set of rules of thumb regarding the mechanical design of anthropomorphic prosthetic hands.

  7. Engineering specification and system design for CAD/CAM of custom shoes: UMC project effort

    NASA Technical Reports Server (NTRS)

    Bao, Han P.

    1991-01-01

    The goal of this project is to supplement the footwear design system of North Carolina State University (NCSU) with a software module to design and manufacture a combination sole. The four areas of concentration were: customization of NASCAD (NASA Computer Aided Design) to the footwear project; use of CENCIT data; computer aided manufacturing activities; and beginning work for the bottom elements of shoes. The task of generating a software module for producing a sole was completed with a demonstrated product realization. The software written in C was delivered to NCSU for inclusion in their design system for custom footwear known as LASTMOD. The machining process of the shoe last was improved using a spiral tool path approach.

  8. Web-based software tool for constraint-based design specification of synthetic biological systems.

    PubMed

    Oberortner, Ernst; Densmore, Douglas

    2015-06-19

    miniEugene provides computational support for solving combinatorial design problems, enabling users to specify and enumerate designs for novel biological systems based on sets of biological constraints. This technical note presents a brief tutorial for biologists and software engineers in the field of synthetic biology on how to use miniEugene. After reading this technical note, users should know which biological constraints are available in miniEugene, understand the syntax and semantics of these constraints, and be able to follow a step-by-step guide to specify the design of a classical synthetic biological system-the genetic toggle switch.1 We also provide links and references to more information on the miniEugene web application and the integration of the miniEugene software library into sophisticated Computer-Aided Design (CAD) tools for synthetic biology ( www.eugenecad.org ).

  9. Formal Specification and Design Techniques for Wireless Sensor and Actuator Networks

    PubMed Central

    Martínez, Diego; González, Apolinar; Blanes, Francisco; Aquino, Raúl; Simo, José; Crespo, Alfons

    2011-01-01

    A current trend in the development and implementation of industrial applications is to use wireless networks to communicate the system nodes, mainly to increase application flexibility, reliability and portability, as well as to reduce the implementation cost. However, the nondeterministic and concurrent behavior of distributed systems makes their analysis and design complex, often resulting in less than satisfactory performance in simulation and test bed scenarios, which is caused by using imprecise models to analyze, validate and design these systems. Moreover, there are some simulation platforms that do not support these models. This paper presents a design and validation method for Wireless Sensor and Actuator Networks (WSAN) which is supported on a minimal set of wireless components represented in Colored Petri Nets (CPN). In summary, the model presented allows users to verify the design properties and structural behavior of the system. PMID:22344203

  10. Claims, Evidence and Achievement Level Descriptors as a Foundation for Item Design and Test Specifications

    ERIC Educational Resources Information Center

    Hendrickson, Amy; Huff, Kristen; Luecht, Ric

    2009-01-01

    [Slides] presented at the Annual Meeting of National Council on Measurement in Education (NCME) in San Diego, CA in April 2009. This presentation describes how the vehicles for gathering student evidence--task models and test specifications--are developed.

  11. Metal-Mediated Affinity and Orientation Specificity in a Computationally Designed Protein Homodimer

    SciTech Connect

    Der, Bryan S.; Machius, Mischa; Miley, Michael J.; Mills, Jeffrey L.; Szyperski, Thomas; Kuhlman, Brian

    2015-10-15

    Computationally designing protein-protein interactions with high affinity and desired orientation is a challenging task. Incorporating metal-binding sites at the target interface may be one approach for increasing affinity and specifying the binding mode, thereby improving robustness of designed interactions for use as tools in basic research as well as in applications from biotechnology to medicine. Here we describe a Rosetta-based approach for the rational design of a protein monomer to form a zinc-mediated, symmetric homodimer. Our metal interface design, named MID1 (NESG target ID OR37), forms a tight dimer in the presence of zinc (MID1-zinc) with a dissociation constant <30 nM. Without zinc the dissociation constant is 4 {micro}M. The crystal structure of MID1-zinc shows good overall agreement with the computational model, but only three out of four designed histidines coordinate zinc. However, a histidine-to-glutamate point mutation resulted in four-coordination of zinc, and the resulting metal binding site and dimer orientation closely matches the computational model (C{alpha} rmsd = 1.4 {angstrom}).

  12. Derivation of Design Loads and Random Vibration specifications for Spacecraft Instruments and Sub-Units

    NASA Astrophysics Data System (ADS)

    Fransen, S.; Yamawaki, T.; Akagi, H.; Eggens, M.; van Baren, C.

    2014-06-01

    After a first estimation based on statistics, the design loads for instruments are generally estimated by coupled spacecraft/instrument sine analysis once an FE-model of the spacecraft is available. When the design loads for the instrument have been derived, the next step in the process is to estimate the random vibration environment at the instrument base and to compute the RMS load at the centre of gravity of the instrument by means of vibro-acoustic analysis. Finally the design loads of the light-weight sub-units of the instrument can be estimated through random vibration analysis at instrument level, taking into account the notches required to protect the instrument interfaces in the hard- mounted random vibration test. This paper presents the aforementioned steps of instrument and sub-units loads derivation in the preliminary design phase of the spacecraft and identifies the problems that may be encountered in terms of design load consistency between low-frequency and high-frequency environments. The SpicA FAR-infrared Instrument (SAFARI) which is currently developed for the Space Infrared Telescope for Cosmology and Astrophysics (SPICA) will be used as a guiding example.

  13. A target-specific electrode and lead design for internal globus pallidus deep brain stimulation.

    PubMed

    Vasques, Xavier; Cif, Laura; Mennessier, Gérard; Coubes, Philippe

    2010-01-01

    In nearly all deep brain stimulation (DBS) applications, the same quadripolar electrode design is used for different anatomical targets even if shape and volume differences exist between nuclei. Taking into account the electrode location within the internal globus pallidus (GPi) and the size of the GPi, 2 electrodes were designed in order to improve the therapeutic benefit, to minimize side effects from DBS and to obtain a more homogeneous electric field distribution. The electrodes were evaluated numerically by using a stereotactic model measuring the correlation between the electric field and the GPi. The model was applied to 26 dystonodyskinetic patients who underwent surgery for a bilateral lead implantation into the posteroventral part of the GPi. The designed electrodes produced a more homogeneous distribution of the electric field than the quadripolar electrode.

  14. Bifunctional chelating agent for the design and development of site specific radiopharmaceuticals and biomolecule conjugation strategy

    DOEpatents

    Katti, Kattesh V.; Prabhu, Kandikere R.; Gali, Hariprasad; Pillarsetty, Nagavara Kishore; Volkert, Wynn A.

    2003-10-21

    There is provided a method of labeling a biomolecule with a transition metal or radiometal in a site specific manner to produce a diagnostic or therapeutic pharmaceutical compound by synthesizing a P.sub.2 N.sub.2 -bifunctional chelating agent intermediate, complexing the intermediate with a radio metal or a transition metal, and covalently linking the resulting metal-complexed bifunctional chelating agent with a biomolecule in a site specific manner. Also provided is a method of synthesizing the --PR.sub.2 containing biomolecules by synthesizing a P.sub.2 N.sub.2 -bifunctional chelating agent intermediate, complexing the intermediate with a radiometal or a transition metal, and covalently linking the resulting radio metal-complexed bifunctional chelating agent with a biomolecule in a site specific manner. There is provided a therapeutic or diagnostic agent comprising a --PR.sub.2 containing biomolecule.

  15. PI/PID controller design based on IMC and percentage overshoot specification to controller setpoint change.

    PubMed

    Ali, Ahmad; Majhi, Somanath

    2009-01-01

    In this work, the normalized Internal Model Control (IMC) filter time constant is designed to achieve a specified value of the maximum sensitivity for stable first and second order plus time delay process models, respectively. Since a particular value of the maximum sensitivity results in an almost constant percentage overshoot to controller setpoint change, an empirical relationship between the normalized IMC filter time constant and percentage overshoot is presented. The main advantage of the proposed method is that only a user-defined overshoot is required to design a PI/PID controller. Simulation examples are given to demonstrate the value of the proposed method.

  16. cry1 genes from Bacillus thuringiensis: specificity determination and implications for primer design.

    PubMed

    Davolos, Camila C; Guidelli-Thuler, Ana M; de Abreu, Irlan L; Sena, Janete A D; Lemos, Manoel V F

    2009-12-01

    Some pest management programs employ PCR to identify cry1 genes from Bacillus thuringiensis to predict bacterial toxicity towards different insect pests. However, due to changes on the mode of action of the Cry proteins, new primers had to be designed to detect the new genes. Therefore, an 'in-silico' study of genetic sequences from five cry1 subclasses was carried out and characterized by molecular tools. The design of new primers allows for more precise selection of B. thuringiensis isolates, helping to better direct the programs employing biological control.

  17. Data-Division-Specific Robustness and Power of Randomization Tests for ABAB Designs

    ERIC Educational Resources Information Center

    Manolov, Rumen; Solanas, Antonio; Bulte, Isis; Onghena, Patrick

    2010-01-01

    This study deals with the statistical properties of a randomization test applied to an ABAB design in cases where the desirable random assignment of the points of change in phase is not possible. To obtain information about each possible data division, the authors carried out a conditional Monte Carlo simulation with 100,000 samples for each…

  18. 24 CFR 3280.904 - Specific requirements for designing the transportation system.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... utilize a fabricated steel frame assembly, upon which the manufactured home structure is constructed, it... integrating the frame function into the manufactured home structure, are acceptable provided that such design... the tire and manufactured home frame or structure to permit unimpeded wheel movement and for...

  19. 24 CFR 3280.904 - Specific requirements for designing the transportation system.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... utilize a fabricated steel frame assembly, upon which the manufactured home structure is constructed, it... integrating the frame function into the manufactured home structure, are acceptable provided that such design... the tire and manufactured home frame or structure to permit unimpeded wheel movement and for...

  20. 24 CFR 3280.904 - Specific requirements for designing the transportation system.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... utilize a fabricated steel frame assembly, upon which the manufactured home structure is constructed, it... integrating the frame function into the manufactured home structure, are acceptable provided that such design... the tire and manufactured home frame or structure to permit unimpeded wheel movement and for...